Science.gov

Sample records for cv cobriza inta

  1. The network of INTA telescopes

    NASA Astrophysics Data System (ADS)

    Cuesta, L.

    2008-06-01

    The Spanish Instituto Nacional de Técnica Aeroespacial has a network of three telescopes located at some of the best places for astronomy in mainland Spain. The first is at the Observatorio de Calar Alto in Almeria, at an altitude of more than 2100 m. The second is near Calatayud in Zaragoza, at the summit of a 1400-m high mountain. The last is on the campus of the Instituto Nacional de Técnica Aerospatial (INTA), in Madrid. The three telescopes are either 40 or 50 cm in diameter and will be available for communications and educational projects.

  2. The four INTA-300 rocket prototypes

    NASA Astrophysics Data System (ADS)

    Calero, J. S.

    1985-03-01

    A development history and performance capability assessment is presented for the INTA-300 'Flamenco' sounding rocket prototype specimens. The Flamenco is a two-stage solid fuel rocket, based on British sounding rocket technology, that can lift 50 km payloads to altitudes of about 300 km. The flight of the first two prototypes, in 1974 and 1975, pointed to vibration problems which reduced the achievable apogee, and the third prototype's flight was marred by a premature detonation that destroyed the rocket. The fourth Flamenco flight, however, yielded much reliable data.

  3. CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains.

    PubMed

    Wright, Patrick R; Georg, Jens; Mann, Martin; Sorescu, Dragos A; Richter, Andreas S; Lott, Steffen; Kleinkauf, Robert; Hess, Wolfgang R; Backofen, Rolf

    2014-07-01

    CopraRNA (Comparative prediction algorithm for small RNA targets) is the most recent asset to the Freiburg RNA Tools webserver. It incorporates and extends the functionality of the existing tool IntaRNA (Interacting RNAs) in order to predict targets, interaction domains and consequently the regulatory networks of bacterial small RNA molecules. The CopraRNA prediction results are accompanied by extensive postprocessing methods such as functional enrichment analysis and visualization of interacting regions. Here, we introduce the functionality of the CopraRNA and IntaRNA webservers and give detailed explanations on their postprocessing functionalities. Both tools are freely accessible at http://rna.informatik.uni-freiburg.de. PMID:24838564

  4. CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains

    PubMed Central

    Wright, Patrick R.; Georg, Jens; Mann, Martin; Sorescu, Dragos A.; Richter, Andreas S.; Lott, Steffen; Kleinkauf, Robert; Hess, Wolfgang R.; Backofen, Rolf

    2014-01-01

    CopraRNA (Comparative prediction algorithm for small RNA targets) is the most recent asset to the Freiburg RNA Tools webserver. It incorporates and extends the functionality of the existing tool IntaRNA (Interacting RNAs) in order to predict targets, interaction domains and consequently the regulatory networks of bacterial small RNA molecules. The CopraRNA prediction results are accompanied by extensive postprocessing methods such as functional enrichment analysis and visualization of interacting regions. Here, we introduce the functionality of the CopraRNA and IntaRNA webservers and give detailed explanations on their postprocessing functionalities. Both tools are freely accessible at http://rna.informatik.uni-freiburg.de. PMID:24838564

  5. Report on upgrade of NIS magnetic observatories by the INTAS infrastructure action CRENEGON

    NASA Astrophysics Data System (ADS)

    Rasson, J. L.; Potapov, A. S.; Korepanov, V. E.; Khomutov, S. Y.; Krasnov, V. M.; Bitterly, J.

    2006-06-01

    We present the overall strategy used to upgrade 9 former USSR observatories to INTERMAGNET standards. The following aspects are discussed: application for funding to the INTAS European ad-hoc agency, choice of observatories to upgrade, choice of the instrumentation to purchase and to install, calibration of the instrumentation and used standards, back-ups, installation of the instrumentation and observatory real estate upgrade, acquisition and communication, training of the observers and documentation, evaluation of the upgrades and management of the project. Several observatories were successfully upgraded and we expect their INTERMAGNET certification soon.

  6. Why Include Bibliometric Analysis in the Activities of a Library Specialized in Astronomy? — Notes From the Libraries of INTA

    NASA Astrophysics Data System (ADS)

    Alonso-Valdivielso, M. Á.; Antonio, E. G.

    2010-10-01

    We describe the different possibilities that several bibliometric studies offer the National Institute of Aerospace Technology (INTA) and the Center for Astrobiology (CAB) libraries. The main object in this paper is to show how the results of these investigations are useful for various matters, such as preservation options, exploitation and diffusion of these results, and facilitating internal information management.

  7. Characterization of IntA, a Bidirectional Site-Specific Recombinase Required for Conjugative Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42

    PubMed Central

    Hernández-Tamayo, Rogelio; Sohlenkamp, Christian; Puente, José Luis; Brom, Susana

    2013-01-01

    Site-specific recombination occurs at short specific sequences, mediated by the cognate recombinases. IntA is a recombinase from Rhizobium etli CFN42 and belongs to the tyrosine recombinase family. It allows cointegration of plasmid p42a and the symbiotic plasmid via site-specific recombination between attachment regions (attA and attD) located in each replicon. Cointegration is needed for conjugative transfer of the symbiotic plasmid. To characterize this system, two plasmids harboring the corresponding attachment sites and intA were constructed. Introduction of these plasmids into R. etli revealed IntA-dependent recombination events occurring at high frequency. Interestingly, IntA promotes not only integration, but also excision events, albeit at a lower frequency. Thus, R. etli IntA appears to be a bidirectional recombinase. IntA was purified and used to set up electrophoretic mobility shift assays with linear fragments containing attA and attD. IntA-dependent retarded complexes were observed only with fragments containing either attA or attD. Specific retarded complexes, as well as normal in vivo recombination abilities, were seen even in derivatives harboring only a minimal attachment region (comprising the 5-bp central region flanked by 9- to 11-bp inverted repeats). DNase I-footprinting assays with IntA revealed specific protection of these zones. Mutations that disrupt the integrity of the 9- to 11-bp inverted repeats abolish both specific binding and recombination ability, while mutations in the 5-bp central region severely reduce both binding and recombination. These results show that IntA is a bidirectional recombinase that binds to att regions without requiring neighboring sequences as enhancers of recombination. PMID:23935046

  8. End-to-end validation process for the INTA-Nanosat-1B Attitude Control System

    NASA Astrophysics Data System (ADS)

    Polo, Óscar R.; Esteban, Segundo; Cercos, Lorenzo; Parra, Pablo; Angulo, Manuel

    2014-01-01

    This paper describes the end-to-end validation process for the Attitude Control Subsystem (ACS) of the satellite INTA-NanoSat-1B (NS-1B). This satellite was launched on July 2009 and it has been fully operative since then. The development of its ACS modules required an exhaustive integration and a system-level validation program. Some of the tests were centred on the validation of the drivers of sensors and actuators and were carried out over the flying model of the satellite. Others, more complex, constituted end-to-end tests where the concurrency of modules, the real-time control requirements and even the well-formedness of the telemetry data were verified. This work presents an incremental and highly automatised way for performing the ACS validation program based on two development suites and an end-to-end validation environment. The validation environment combines a Flat Satellite (FlatSat) configuration and a real-time emulator working in closed-loop. The FlatSat is built using the NS-1B Qualification Model (QM) hardware and it can run a complete version of the on-board software with the ACS modules fully integrated. The real-time emulator, running on an industrial PC, samples the actuation signals and emulates the sensors signals to close the control loop with the FlatSat. This validation environment constitutes a low-cost alternative to the classical three axes tilt table, with the advantage of being easily configured for working under specific orbit conditions, in accordance with any of the selected tests. The approach has been successfully applied to the NS-1B in order to verify different ACS modes under multiple orbit scenarios, providing an exhaustive coverage and reducing the risk of eventual errors during the satellite's lifetime. The strategy was applied also during the validation of the maintenance and reconfiguration procedures required once the satellite was launched. This paper describes in detail the complete ACS validation process that was

  9. CV 100--Still Going Strong

    ERIC Educational Resources Information Center

    Abrahams, A. L.

    1976-01-01

    Describes results of a study that used CV 100, a fuel additive for use in oil-fired heating systems, on a trial basis in 12 Ontario schools. The test showed an average 12 percent reduction in fuel costs in the schools using CV 100. (JG)

  10. Digitization, Integration and Preservation of Technical and Historical Information: The Case of INTA, the National Institute for Aerospace Technique of Spain

    NASA Astrophysics Data System (ADS)

    Merida Martín, F.; Paz Otero, S.

    2007-10-01

    During the last two years the INTA -- National Institute for Aerospace Technique -- library has been improving different areas related to the information management processes, such as those related to cataloguing, dissemination of technical information, centralization at the Library of all relevant documents and information applicable to scientific research within our organization, implementation of library web services, etc. As part of these processes of modernization of services that the INTA Library is carrying out, a project of digitization of both technical documentation and historical records of the Institute has been defined. The goal is to achieve the total digitization of technical documents and historical papers through the year 2006, and provide access for the resulting electronic collection to the Spanish aerospace community. For the development of the project a deep study of the state of the art in digitization and preservation matters has been conducted. That study covers the different aspects of such a project that could be experienced, such as the risk of data loss, the bandwidth needed to guarantee access to this huge quantity of electronic documentation, the fragility of the digital media, the rapid obsolescence of hardware and software, etc. Also the project is going to assume the new reality of documents that are not originating in paper format, but are digital-born, and how to integrate all the electronic documents in one system, fulfilling the same standards and using the same available technology.

  11. The New INTA High-Range Standard Humidity Generator and Its Comparison with the Austrian National Humidity Standard Maintained at BEV/E+E

    NASA Astrophysics Data System (ADS)

    Benyon, Robert; Mitter, Helmat

    2008-10-01

    A EUROMET collaborative project has been set up between Instituto Nacional de Técnica Aeroespacial (INTA) and E+E ELEKTRONIK Ges.m.b.H, the two designated laboratories of the Spanish and Austrian National Metrology Institutes, Centro Español de Metrología (CEM) and Bundesamt für Eich- und Vermessungswesen (BEV), respectively. The objective of the project is to provide INTA with a new standard that covers the dew-point temperature range from - 27°C to +90°C with a gas flow up to 5 L· min-1 in the “two-pressure” mode, extended to 95°C when operated as a continuous flow “single-pressure” generator, and investigate the importance of the enhancement factors in the uncertainty estimations used in support of the participants’ calibration and measurement capabilities (CMC) (The CIPM Mutual Recognition Arrangement, http://www.bipm.fr/en/cipm-mra/ ). The equivalence of the Spanish and Austrian national standards is also to be evaluated, further supporting the outcomes of the Key Comparisons, in which both have already participated. The preliminary results obtained to date are reported and discussed in the context of the project and the consistency of the declared CMC’s.

  12. Compensatory Lengthening in Karok and CV Phonology.

    ERIC Educational Resources Information Center

    Chung, Young Hee

    A study of Karok, an American Indian language spoken in northern California, provides an argument for CV theory over moraic theory from compensatory lengthening. In a previous study, moraic theory is argued to be superior to CV phonology in accounting for compensatory lengthening; it is shown here that compensatory lengthening in Karok cannot be…

  13. Complete sequence of three plasmids from Bacillus thuringiensis INTA-FR7-4 environmental isolate and comparison with related plasmids from the Bacillus cereus group.

    PubMed

    Amadio, Ariel F; Benintende, Graciela B; Zandomeni, Rubén O

    2009-11-01

    Bacillus thuringiensis is an insect pathogen used worldwide as a bioinsecticide. It belongs to the Bacillus cereus sensu lato group as well as Bacillus anthracis and B. cereus. Plasmids from this group of organisms have been implicated in pathogenicity as they carry the genes responsible for different types of diseases that affect mammals and insects. Some plasmids, like pAW63 and pBT9727, encode a functional conjugation machinery allowing them to be transferred to a recipient cell. They also share extensive homology with the non-functional conjugation apparatus of pXO2 from B. anthracis. In this study we report the complete sequence of three plasmids from an environmental B. thuringiensis isolate from Argentina, obtained by a shotgun sequencing method. We obtained the complete nucleotide sequence of plasmids pFR12 (12,095bp), pFR12.5 (12,459bp) and pFR55 (55,712bp) from B. thuringiensis INTA-FR7-4. pFR12 and pFR12.5 were classified as cryptic as they do not code for any obvious functions besides replication and mobilization. Both small plasmids were classified as RCR plasmids due to similarities with the replicases they encode. Plasmid pFR55 showed a structural organization similar to that observed for plasmids pAW63, pBT9727 and pXO2. pFR55 also shares a tra region with these plasmids, containing genes related to T4SS and conjugation. A comparison between pFR55 and conjugative plasmids led to the postulation that pFR55 is a conjugative plasmid. Genes related to replication functions in pFR55 are different to those described for plasmids with known complete sequences. pFR55 is the first completely sequenced plasmid with a replication machinery related to that of ori44. The analysis of the complete sequence of plasmids from an environmental isolate of B. thuringiensis permitted the identification of a near complete conjugation apparatus in pFR55, resembling those of plasmids pAW63, pBT9727 and pXO2. The availability of this sequence is a step forward in the study

  14. CV and CM chondrite impact melts

    NASA Astrophysics Data System (ADS)

    Lunning, Nicole G.; Corrigan, Catherine M.; McSween, Harry Y.; Tenner, Travis J.; Kita, Noriko T.; Bodnar, Robert J.

    2016-09-01

    Volatile-rich and typically oxidized carbonaceous chondrites, such as CV and CM chondrites, potentially respond to impacts differently than do other chondritic materials. Understanding impact melting of carbonaceous chondrites has been hampered by the dearth of recognized impact melt samples. In this study we identify five carbonaceous chondrite impact melt clasts in three host meteorites: a CV3red chondrite, a CV3oxA chondrite, and a regolithic howardite. The impact melt clasts in these meteorites respectively formed from CV3red chondrite, CV3oxA chondrite, and CM chondrite protoliths. We identified these impact melt clasts and interpreted their precursors based on their texture, mineral chemistry, silicate bulk elemental composition, and in the case of the CM chondrite impact melt clast, in situ measurement of oxygen three-isotope signatures in olivine. These impact melts typically contain euhedral-subhedral olivine microphenocrysts, sometimes with relict cores, in glassy groundmasses. Based on petrography and Raman spectroscopy, four of the impact melt clasts exhibit evidence for volatile loss: these melt clasts either contain vesicles or are depleted in H2O relative to their precursors. Volatile loss (i.e., H2O) may have reduced the redox state of the CM chondrite impact melt clast. The clasts that formed from the more oxidized precursors (CV3oxA and CM chondrites) exhibit phase and bulk silicate elemental compositions consistent with higher intrinsic oxygen fugacities relative to the clast that formed from a more reduced precursor (CV3red chondrite). The mineral chemistries and assemblages of the CV and CM chondrite impact melt clasts identified here provide a template for recognizing carbonaceous chondrite impact melts on the surfaces of asteroids.

  15. Iterative contextual CV model for liver segmentation

    NASA Astrophysics Data System (ADS)

    Ji, Hongwei; He, Jiangping; Yang, Xin

    2014-01-01

    In this paper, we propose a novel iterative active contour algorithm, i.e. Iterative Contextual CV Model (ICCV), and apply it to automatic liver segmentation from 3D CT images. ICCV is a learning-based method and can be divided into two stages. At the first stage, i.e. the training stage, given a set of abdominal CT training images and the corresponding manual liver labels, our task is to construct a series of self-correcting classifiers by learning a mapping between automatic segmentations (in each round) and manual reference segmentations via context features. At the second stage, i.e. the segmentation stage, first the basic CV model is used to segment the image and subsequently Contextual CV Model (CCV), which combines the image information and the current shape model, is iteratively performed to improve the segmentation result. The current shape model is obtained by inputting the previous automatic segmentation result into the corresponding self-correcting classifier. The proposed method is evaluated on the datasets of MICCAI 2007 liver segmentation challenge. The experimental results show that we obtain more and more accurate segmentation results by the iterative steps and satisfying results are obtained after about six iterations. Also, our method is comparable to the state-of-the-art work on liver segmentation.

  16. EARTH, MOON, SUN, AND CV ACCRETION DISKS

    SciTech Connect

    Montgomery, M. M.

    2009-11-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if

  17. Earth, Moon, Sun, and CV Accretion Disks

    NASA Astrophysics Data System (ADS)

    Montgomery, M. M.

    2009-11-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if

  18. MIZEX, 1984, NASA CV-990 flight report

    NASA Technical Reports Server (NTRS)

    1985-01-01

    During June/July 1984, the NASA CV-990 Airborne Laboratory was utilized in a mission to overly the Fram Strait/East Greenland Sea marginal ice zone (MIZ) during the main summer marginal ice zone experiment (MIZEX '84). The eight data flights were coordinated where possible with overpasses of the Nimbus-7 satellite, and with measurement of sea ice, open ocean, and atmospheric properties at the surface. The surface research teams were based on seven research vessels, some with helicopters: (1) M/V Kvitbjorn, (2) M/V Polarqueen; (3) M/S Haakon Mosby; (4) a M/S H.U. Sverdrup, all from Norway; (5) F/S Polarstern from the Federal Republic of Germany; and (6) the USNS Lynch from the USA. There were also coordinated flights with the NRL P3, NOAA P3, Canadian CV580, and the French B-17 during the overlap portions of their respective missions. Analysis of the real-time data acquired during the mission and uncalibrated data stored on tape has served to indicate the mission was over 90% successful.

  19. Cloning and sequencing of Duck circovirus (DuCV).

    PubMed

    Hattermann, K; Schmitt, C; Soike, D; Mankertz, A

    2003-12-01

    The genome of Duck circovirus (DuCV) is circular and 1996 nts in size. Two major open reading frames were identified, encoding the replicase (V1) and the capsid protein (C1). A stem-loop structure comprising the nonamer 5'-TATTATTAC, conserved in all circo-, nano- and geminiviruses, was found. Unique to DuCV, the region between the 3'-ends of the rep and cap gene contains four repeats of a 44-bp sequence. Phylogenetic analysis shows close relation of DuCV with Goose circovirus and suggests classification of DuCV as a new member of the genus Circovirus of the virus family Circoviridae. PMID:14648300

  20. NASA/ESA CV-990 spacelab simulation

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Due to interest in the application of simplified techniques used to conduct airborne science missions at NASA's Ames Research Center, a joint NASA/ESA endeavor was established to conduct an extensive Spacelab simulation using the NASA CV-990 airborne laboratory. The scientific payload was selected to perform studies in upper atmospheric physics and infrared astronomy with principal investigators from France, the Netherlands, England, and several groups from the United States. Communication links between the 'Spacelab' and a ground based mission operations center were limited consistent with Spacelab plans. The mission was successful and provided extensive data relevant to Spacelab objectives on overall management of a complex international payload; experiment preparation, testing, and integration; training for proxy operation in space; data handling; multiexperimenter use of common experimenter facilities (telescopes); multiexperiment operation by experiment operators; selection criteria for Spacelab experiment operators; and schedule requirements to prepare for such a Spacelab mission.

  1. Protective effect of CV247 against cisplatin nephrotoxicity in rats.

    PubMed

    Máthé, C; Szénási, G; Sebestény, A; Blázovics, A; Szentmihályi, K; Hamar, P; Albert, M

    2014-08-01

    CV247 (CV), an aqueous mixture of copper (Cu) and manganese (Mn) gluconates, vitamin C and sodium salicylate increased the antitumour effects of cisplatin (CDPP; cis-diamminedichloroplatinum) in vitro. We hypothesized that the antioxidant and cyclooxygenase-2 (COX-2; prostaglandin-endoperoxide synthase 2) inhibitory components of CV can protect the kidneys from CDPP nephrotoxicity in rats. CDPP (6.5 mg/kg, intraperitoneally) slightly elevated serum creatinine (Crea) and blood urea nitrogen (BUN) 12 days after treatment. Kidney histology demonstrated extensive tubular epithelial damage and COX-2 immunoreactivity increased 14 days after treatment. A large amount of platinum (Pt) accumulated in the kidney of CDPP-treated rats. Furthermore, CDPP decreased renal iron (Fe), molybdenum (Mo), zinc (Zn), Cu and Mn concentrations and increased plasma Fe and Cu concentrations. CDPP elevated plasma free radical concentration. Treatment with CV alone for 14 days (twice 3 ml/kg/day orally) did not influence these parameters. Chronic CV administration after CDPP reduced renal histological damage and slightly decreased COX-2 immunoreactivity, while failed to prevent the increase in Crea and BUN levels. Blood free radical concentration was reduced, that is, CV improved redox homeostasis. CV restored plasma Fe and renal Fe, Mo and Zn, while decreased Pt and elevated Cu and Mn concentrations in the kidney. Besides the known synergistic antitumour effects with CDPP, CV partially protected the kidneys from CDPP nephrotoxicity probably through its antioxidant effect. PMID:23653282

  2. Graphite whiskers in CV3 meteorites.

    PubMed

    Fries, Marc; Steele, Andrew

    2008-04-01

    Graphite whiskers (GWs), an allotrope of carbon that has been proposed to occur in space, have been discovered in three CV-type carbonaceous chondrites via Raman imaging and electron microscopy. The GWs are associated with high-temperature calcium-aluminum inclusion (CAI) rims and interiors, with the rim of a dark inclusion, and within an inclusion inside an unusual chondrule that bears mineralogy and texture indicative of high-temperature processing. Current understanding of CAI formation places their condensation, and that of associated GWs, relatively close to the Sun and early in the condensation sequence of protoplanetary disk materials. If this is the case, then it is a possibility that GWs are expelled from any young solar system early in its history, thus populating interstellar space with diffuse GWs. Graphite whiskers have been postulated to play a role in the near-infrared (near-IR) dimming of type Ia supernovae, as well as in the thermalization of both the cosmic IR and microwave background and in galactic center dimming between 3 and 9 micrometers. Our observations, along with the further possibility that GWs could be manufactured during supernovae, suggest that GWs may have substantial effects in observational astronomy. PMID:18309047

  3. INTA-SAR real-time processor

    SciTech Connect

    Gomez, B.; Leon, J.

    1996-10-01

    This paper presents the INTASAR real time processor development based on a DSP open architecture for processing Synthetic Aperture Radar (SAR) signal. The final designed architecture must consider three different constraints sources: (a) SAR signal characteristics : high dynamic range, and complex SAR imaging algorithms with high computational load (multiprocessing is convenient). (b) Flexible: in connectivity and algorithms to be programmed. (c) Suitable: for on-board and ground working. The real time constraints will be defined by the image acquisition time, within it the INTASAR system will process the rawdata image and finally presents the results in the system monitor. At ground, however, the real time processing is not a constraint, but the high quality image is. The first algorithm implemented in the system was a Range - Doppler one. With the multiprocessor architecture selected, a pipeline processing method is used. 17 refs., 4 figs., 2 tabs.

  4. Rare Earth Element Complementarity in CO and CV Chondrites

    NASA Astrophysics Data System (ADS)

    Crapster-Pregont, E. J.; Friedrich, J. M.; Ebel, D. S.

    2014-09-01

    LREE/HREE ratios in each component of CO and CV chondrites combine to produce complementary, flat bulk REE pattern, while individually yielding insight into mineral controls and precursor characteristics.

  5. Insights from High Precision Oxygen Isotopic Analyses of Reduced CV Meteorite Separates: CV Matrix Homogeneity and Preservation of Nebular Heterogeneity

    NASA Astrophysics Data System (ADS)

    Dyl, K. A.; Young, E. D.

    2011-03-01

    The oxygen-isotopic composition of matrix from reduced CVs Efremovka and Vigarano has been measured and is indistinguishable from Allende. This indicates a similar nebular history and a preservation of different nebular conditions within reduced CV meteorites.

  6. Opaque Assemblages in CK and CV Carbonaceous Chondrites

    NASA Technical Reports Server (NTRS)

    Neff, K. E.; Righter, K.

    2006-01-01

    CK carbonaceous chondrites are the only group of carbonaceous chondrites that exhibit thermal metamorphism. As a result, CKs display features of metamorphism such as silicate darkening, recrystallization and shock veins. Calcium Aluminum Inclusions and Fe-Ni metal are rare. CV carbonaceous chondrites are unequilibrated and have two subgroups; oxidized and reduced. The CV and CK carbonaceous chondrite groups have been compared to each other often because of petrographic similarities, such as overlapping oxygen isotopic ratios. Scientists have suggested the two groups of carbonaceous chondrites formed from the same parent body and CKs are equilibrated CV chondrites [1, 2]. The oxidized CV group has been most closely related to CKs. This study examines the petrology and mineralogy of CKs and CVs focusing on opaque minerals found in the meteorites. Using the oxide, metal and sulfide assemblages, constraints can be placed on the temperature and oxygen fugacity at which the meteorites equilibrated. The temperature and oxygen fugacity of the CK and CV chondrites can be compared in order to help define their formation history.

  7. Anonymous voting for multi-dimensional CV quantum system

    NASA Astrophysics Data System (ADS)

    Rong-Hua, Shi; Yi, Xiao; Jin-Jing, Shi; Ying, Guo; Moon-Ho, Lee

    2016-06-01

    We investigate the design of anonymous voting protocols, CV-based binary-valued ballot and CV-based multi-valued ballot with continuous variables (CV) in a multi-dimensional quantum cryptosystem to ensure the security of voting procedure and data privacy. The quantum entangled states are employed in the continuous variable quantum system to carry the voting information and assist information transmission, which takes the advantage of the GHZ-like states in terms of improving the utilization of quantum states by decreasing the number of required quantum states. It provides a potential approach to achieve the efficient quantum anonymous voting with high transmission security, especially in large-scale votes. Project supported by the National Natural Science Foundation of China (Grant Nos. 61272495, 61379153, and 61401519), the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20130162110012), and the MEST-NRF of Korea (Grant No. 2012-002521).

  8. Aqueous alteration in the Kaba CV3 carbonaceous chondrite

    NASA Technical Reports Server (NTRS)

    Keller, Lindsay P.; Buseck, Peter R.

    1990-01-01

    Results from TEM and SEM examinations of the Kaba CV3 carbonaceous chondrite are presented, showing that the chondrules and the matrix of Kaba have undergone pervasive low-temperature aqueous alteration, resulting in the formation of Fe-bearing saponite from glass and enstatite in chondrules, and from anhydrous silicates in matrix. The alteration products in Kaba were found to resemble those in other aqueously altered carbonaceous chondrites such as the Mokoia CV3 and in Orgueil CI chondrites and Y-82162 chondrites. However, Kaba lacks the abundant high-Al phyllosilicates, reported for CAIs from Mokoia, and the serpentine and ferrihydrite, found in Orgueil.

  9. Whirling Dervish Dynamos: Magnetic Activity in CV Secondaries

    NASA Astrophysics Data System (ADS)

    Saar, Steven

    2003-07-01

    The mass-losing secondary stars of cataclysmic variables {CVs} are the most rapidly rotating cool dwarfs observable. Other rapid rotators show a maximal, "saturated" level of magnetic activity {e.g., X-ray emission}, but there are hints from contact binaries and young clusters that activity may be suppressed at the highest rotation rates. CV secondaries are thus important probes of magnetic dynamos at rotational extremes. Implications for CV evolution {e.g., the ``period gap", accretion variability} may also be profound. Unfortunately, study of CV secondaries is hampered by pesky accretion-related phenomena and reflection effects. As a result, little systematic work has been done. To explore activity in these stars, we therefore propose to study far-UV spectra of AM Her-type systems {which have no accretion disks} in deep photometric minima in which accretion is shut off. Magnetic-related emission from the secondary will be separated {in velocity} from residual accretion emission by observations near quadratures. Lower chromospheric irradiation due to the white dwarf primary will be removed by modeling, yielding the true level of magnetic activity on the secondary. We will compare the results to other dMe stars and draw implications for magnetic dynamos and activity at rotational extremes, and for CV evolution and behavior.

  10. Draft Genome Sequence of Chromobacterium violaceum Strain CV017

    PubMed Central

    Wang, Xiaofei; Hinshaw, Kara C.; Macdonald, Stuart J.

    2016-01-01

    We announce the draft genome sequence for Chromobacterium violaceum strain CV017, used as a model and tool to understand acyl-homoserine lactone-dependent quorum sensing. The assembly consists of 4,774,638-bp contained in 211 scaffolds. PMID:26941151

  11. Computational simulation of CV combination preferences in babbling

    PubMed Central

    Nam, Hosung; Goldstein, Louis M.; Giulivi, Sara; Levitt, Andrea G.; Whalen, D. H.

    2013-01-01

    There is a tendency for spoken consonant-vowel (CV) syllables, in babbling in particular, to show preferred combinations: labial consonants with central vowels, alveolars with front, and velars with back. This pattern was first described by MacNeilage and Davis, who found the evidence compatible with their “frame-then-content” (F/C) model. F/C postulates that CV syllables in babbling are produced with no control of the tongue (and therefore effectively random tongue positions) but systematic oscillation of the jaw. Articulatory Phonology (AP; Browman & Goldstein) predicts that CV preferences will depend on the degree of synergy of tongue movements for the C and V. We present computational modeling of both accounts using articulatory synthesis. Simulations found better correlations between patterns in babbling and the AP account than with the F/C model. These results indicate that the underlying assumptions of the F/C model are not supported and that the AP account provides a better and account with broader coverage by showing that articulatory synergies influence all CV syllables, not just the most common ones. PMID:24496111

  12. Evidence from Chondrule Shapes and Modes for Shock Deformation in Reduced CV3 Chondrites Leoville and Efreomovka

    NASA Astrophysics Data System (ADS)

    Fagan, T. J.; Aoki, R.

    2015-07-01

    The reduced CV3 chondrites Efremovka and Leoville are characterized by (1) more elongate chondrules, and (2) lower matrix abundances compared to the oxidized CV3 Allende. Both observations can be explained by shock deformation of reduced CV3s.

  13. Axtrell, a new CV3 chondrite find from Texas

    NASA Technical Reports Server (NTRS)

    Simon, S. B.; Grossman, L.; Casanova, I.; Symes, S.; Benoit, P.; Sears, D. W. G.; Wacker, J. F.

    1995-01-01

    We describe a previously unreported meteorite found in Axtell, Texas, in 1943. Based on the mineralogical composition and texture of its matrix and the sizes and abundance of chondrules, we classify it as a CV3 carbonaceous chondrite. The dominant opaque phase in the chondrules is magnetite, and that in refractory inclusions is Ni-rich metal (awaruite). Axtell, therefore, belongs to the oxidized subgroup of CV3 chondrites, although unlike Allende it escaped strong sulfidation. The meteorite bears a strong textural resemblance to Allende, and its chondrule population and matrix appear to be quite similar to those of Allende, but its refractory inclusions, thermoluminescence properties, and cosmogenic Co-60 abundances are not. Our data are consistent with a terrestrial age for Axtell of approximately 100 years and a metamorphic grade slightly lower than that of Allende.

  14. Spectroscopic Classification of ASASSN-16do as a CV

    NASA Astrophysics Data System (ADS)

    Strader, Jay; Chomiuk, Laura; Shishkovsky, Laura

    2016-04-01

    We obtained an optical spectrum of ASASSN-16do (ATel #8888) on UT April 17.07 with the Goodman Spectrograph on the SOAR telescope. The source has a blue continuum and broad double-peaked Balmer and He 5875 emission at z~0, with an H-alpha FWHM of about 2400 km/s. This value is high for a CV and suggests the source is observed close to edge-on.

  15. Magnetic CVs in the UCT CCD CV Survey

    NASA Astrophysics Data System (ADS)

    Woudt, P. A.; Warner, B.

    2004-12-01

    An overview is given of all the magnetic CVs found in the UCT CCD CV Survey (Woudt & Warner 2001, 2002, 2003a). We have identified eight new candidate Intermediate Polars (IP), of which six are classical novae (RR Cha, DD Cir, AP Cru, V697 Sco, V373 Sct, and RX J1039.7-0507). The two other candidate IPs are Aqr1 (2236+0052) and RX J0944.5+0357. In addition, there are two probable Polars, namely V351 Pup (= Nova Puppis 1991) and FIRST J102347.6+003841.

  16. The All-Sky Automated Survey for Supernovae CV Patrol

    NASA Astrophysics Data System (ADS)

    Davis, Alexandra Bianca; Shappee, Benjamin John; Archer Shappee, Bartlett; ASAS-SN

    2015-01-01

    Even in the modern era, only human eyes scan the entire optical sky for the violent, variable, and transient events that shape our universe. The "All Sky Automated Survey for Supernovae" (ASAS-SN or "Assassin") is changing this by monitoring the extra-galactic sky down to V~17 mag every 2-3 days using multiple telescopes, hosted by Las Cumbres Observatory Global Telescope Network, in the northern and southern hemispheres. By far the most common events observed by ASAS-SN are the Galactic transients. Since April 2013 ASAS-SN has identified over 180 new cataclysmic variable stars and announced over 260 new outbursts of known CVs. To make our data available to the CV community in 'real time', we have launched an automated 'CV Patrol' to monitor known CVs for outbursts as a useful tool for both professional and amateurs astronomers. It is a long term goal of ASAS-SN to make all our data public in real-time, and this patrol will serve as a framework for future ASAS-SN data releases.

  17. CV-6209, a highly potent antagonist of platelet activating factor in vitro and in vivo.

    PubMed

    Terashita, Z; Imura, Y; Takatani, M; Tsushima, S; Nishikawa, K

    1987-07-01

    2-[N-acetyl-N-(2-methoxy-3-octadecylcarbamoyloxypropoxycarbonyl) aminomethyl]-1-ethylpyridinium chloride (CV-6209) inhibited aggregation of rabbit and human platelets induced by platelet activating factor (PAF) with the IC50 values of 7.5 X 10(-8) and 1.7 X 10(-7) M, respectively, and had little effects on the aggregation induced by arachidonic acid, ADP and collagen. The inhibitory effect of CV-6209 on the PAF-induced rabbit platelet aggregation was 104, 9, 8 and 3 times more potent than the PAF antagonists CV-3988, ONO-6240, Ginkgolide B and etizolam, respectively. CV-6209 inhibited [3H]serotonin release from rabbit platelets stimulated with PAF (3 X 10(-8) M) with a similar potency as the inhibition on the platelet aggregation. CV-6209 inhibited PAF (0.3 microgram/kg i.v.)-induced hypotension in rats (ED50, 0.009 mg/kg i.v.) with no effect on the hypotension induced by arachidonic acid, histamine, bradykinin and isoproterenol. CV-6209 (1 mg/kg) inhibited slightly the acetylcholine-induced hypotension. In rats, post-treatment with CV-6209 reversed the PAF (1 microgram/kg i.v.)-induced hypotension rapidly (ED50, 0.0046 mg/kg i.v.); CV-6209 was 74, 20, 185 and over 2100 times more potent than CV-3988, ONO-6240, Ginkgolide B and etizolam, respectively. Thus, the relative potency of the anti-PAF action of PAF analog (CV-6209, CV-3988 and ONO-6240) differed little between the inhibition of PAF-induced platelet aggregation and the reversal of PAF-induced hypotension, but that of nonPAF analogs (Ginkgolide B and etizolam) differed greatly with these assay systems, when standardized with CV-6209.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:3612533

  18. So you want to be a specialist registrar?--What to put in your CV.

    PubMed

    Ellis, P E; Ellis, S G S; O'Brien, K D; Joshi, R I

    2002-02-01

    Dentists applying to a specialist training programme often receive conflicting advice over what to put in their curriculum vitae (CV). We conducted a survey of the Training Programme Directors of the dental specialties to determine what aspects of CV content and presentation styles are considered important. This has allowed us to construct guidelines for what to put in a CV. Recently, structured application forms have become increasingly popular and may be a more objective way to carry out the shortlisting process. The guidelines presented could also be used as a framework for medical personnel departments if structured application forms eventually replace the CV. PMID:11865819

  19. Cytotoxic diterpenoids from Jatropha curcas cv. nigroviensrugosus CY Yang Roots.

    PubMed

    Liu, JieQing; Yang, YuanFeng; Xia, JianJun; Li, XuYang; Li, ZhongRong; Zhou, Lin; Qiu, MingHua

    2015-09-01

    An investigation of phytochemicals from the roots of Jatropha curcas cv. nigroviensrugosus resulted in the isolation of twenty diterpenoids, including lathyranlactone, an unusual diterpenoid lactone possessing a 5/13/3 tricyclic skeleton, jatrocurcasenones A-E and jatrophodiones B-E, as well as 10 known analogues. All isolates were evaluated for cytotoxicity against the HL-60, SMMC-772, A-549, MCF-7 and SW480 human tumor cell lines using the MTS viability assay. Four of the known analogues showed cytotoxic activity in these cell lines, with IC50 values ranging from 2.0 to 23.0 μM. Moreover, the assessment of their cytotoxic structure-activity relationships showed the epoxy ring between C-5 and C-6 and the hydroxyl group at C-2 were the key functionalities for cytotoxicity. PMID:26209936

  20. Death of pastures syndrome: tissue changes in Urochloa hybrida cv. Mulato II and Urochloa brizantha cv. Marandu.

    PubMed

    Ribeiro-Júnior, N G; Ariano, A P R; Silva, I V

    2016-07-11

    The quality of forage production is a prerequisite to raising livestock. Therefore, income losses in this activity, primarily cattle raising, can result in the impossibility of economic activity. Through the qualitative and quantitative anatomical study of Urochloa hybrida cv. Mulato II and U. brizantha cv. Marandu, we searched for descriptions and compared changes in the individual vegetative body from populations with death syndrome pastures (DPS). Specimens were collected at different physiological stages from farms in northern Mato Grosso. After collection, the individuals were fixed in FAA50 and stored in 70% alcohol. Histological slides were prepared from the middle third of the sections of roots, rhizomes, and leaves, and the proportions and characteristics of tissues were evaluated in healthy, intermediate, and advanced stages of DPS. Changes were compared between cultivars. With the advancement of the syndrome, the following changes were observed: a more marked decrease in the length of roots in U. hybrida; disorganization of the cortical region of the roots and rhizome cultivars; fungal hyphae in roots and aerenchyma formation in U. hybrida; a decrease in sclerenchyma fiber proportions in roots and leaves; sclerification of the epidermis of U. brizantha rhizomes; and an increase in pericyclic fibers in U. hybrida. Furthermore, there was a decrease in the volume of epidermal cells of the abaxial face of the leaves of both cultivars, with a greater reduction in U. hybrida; a gradual decrease in thickness in the midrib of leaves similar to leaf mesophyll; conduction system obstructions; partial or total cell lysis in roots and rhizomes affected by the syndrome. Obstructions in sieve tube element and companion cells, and sometimes obstruction in xylem vessel elements. The evolution of DPS in cultivars was similar, but there were variations, arising probably from the physiological response to stress, such as aerenchyma formation in the root and increased

  1. Ryegrass cv. Lema and guava cv. Paluma biomonitoring suitability for estimating nutritional contamination risks under seasonal climate in Southeastern Brazil.

    PubMed

    Bulbovas, Patricia; Camargo, Carla Z S; Domingos, Marisa

    2015-08-01

    The risks posed by nutrient deposition due to air pollution on ecosystems and their respective services to human beings can be appropriately estimated by bioindicator plants when they are well acclimated to the study region environmental conditions. This assumption encouraged us to comparatively evaluate the accumulation potential of ryegrass cv. Lema and guava cv. Paluma macro and micronutrients. We also indicated the most appropriate species for biomonitoring nutrient contamination risks in tropical areas of Southeastern Brazil, which are characterized by marked dry and wet seasons and complex mixtures of air pollutants from different sources (industries, vehicle traffic and agriculture). The study was conducted in 14 sites with different neighboring land uses, within the Metropolitan Region of Campinas, central-eastern region of São Paulo State. The exposure experiments with ryegrass and guava were consecutively repeated 40 (28 days each) and 12 (84 days each) times, respectively, from Oct/2010 to Sept/2013. Macro and micronutrients were analyzed and background concentrations and enrichment ratios (ER) were estimated to classify the contamination risk within the study region. Significantly higher ER suggested that ryegrass were the most appropriate accumulator species for N, S, Mg, Fe, Mn, Cu and Zn deposition and guava for K, Ca, P and B deposition. Based on these biomonitoring adjustments, we concluded that the nutrient deposition was spatially homogeneous in the study area, but clear seasonality in the contamination risk by nutritional inputs was evidenced. Significantly higher contamination risk by S, Fe, K and B occurred during the dry season and enhanced contamination risk by Mn, Cu and Zn were highlighted during the wet season. Distinctly high contamination risk was estimated for S, Fe and Mn in several exposure experiments. PMID:25938695

  2. Rat and poultry feeding studies with soybean meal produced from imidazolinone-tolerant (CV127) soybeans.

    PubMed

    He, Xiaoyun; de Brum, Paulo A R; Chukwudebe, Amechi; Privalle, Laura; Reed, Andrew; Wang, Yanqing; Zhou, Cui; Wang, Cuiyan; Lu, Jing; Huang, Kunlun; Contri, Daniela; Nakatani, Andreia; de Avila, Valdir S; Klein, Claudete H; de Lima, Gustavo J M M; Lipscomb, Elizabeth A

    2016-02-01

    The safety and nutritional properties of CV127 soybeans were evaluated in rat and broiler feeding studies. Some episodic differences were observed between rats fed CV127, Conquista, and the standard diet for the endpoints examined. None of these differences were considered treatment related, adverse, or biologically meaningful. In general, birds fed diets containing CV127, Conquista, or Monsoy 8001 showed no significant differences in growth and performance response variables. Chickens fed diets containing Coodetec 217 had lower body weight and weight gain for all developmental periods compared to CV127, but no significant differences were found in feed conversion for the two diets during any development period. The results of both feeding studies demonstrate that CV127 soybeans are as safe, wholesome, and nutritionally valuable as the other soybean meals tested, including those varieties for which histories of safe use have been established and well documented. PMID:26699944

  3. Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons.

    PubMed

    Mankertz, A; Hattermann, K; Ehlers, B; Soike, D

    2000-01-01

    The complete nucleotide sequence of columbid circovirus (CoCV) isolated from pigeons is described. CoCV was amplified using a consensus primer PCR approach directed against conserved sequences within the rep genes of vertebrate circoviruses. The genome of CoCV is circular and 2037 nt in size. It displays 55% homology to the genome of psittacine beak and feather disease virus and is more distantly related (< 40% homology) to porcine circovirus type 1 and 2. Two major open reading frames were identified, encoding the replicase and the putative capsid protein of CoCV. A region similar to the origin of replication of other circoviruses was found: it encompasses a stem-loop structure with the nonamer 5'-TAGTATTAC, conserved in circo-, nano- and geminiviruses. Phylogenetic analyses suggest classification of CoCV as member of the genus Circovirus of the virus family Circoviridae. PMID:11205099

  4. Seven health physics calculator programs for the HP-41CV

    SciTech Connect

    Rittmann, P.D.

    1984-08-01

    Several user-oriented programs for the Hewlett-Packard HP-41CV are explained. The first program builds, stores, alters, and ages a list of radionuclides. This program only handles single- and double-decay chains. The second program performs convenient conversions for the six nuclides of concern in plutonium handling. The conversions are between mass, activity, and weight percents of the isotopes. The source can be aged and/or neutron generation rates can be computed. The third program is a timekeeping program that improves the process of manually estimating and tracking personnel exposure during high dose rate tasks by replacing the pencil, paper, and stopwatch method. This program requires a time module. The remaining four programs deal with computations of time-integrated air concentrations at various distances from an airborne release. Building wake effects, source depletion by ground deposition, and sector averaging can all be included in the final printout of the X/Q - Hanford and X/Q - Pasquill programs. The shorter versions of these, H/Q and P/Q, compute centerline or sector-averaged values and include a subroutine to facilitate dose estimation by entering dose factors and quantities released. The horizontal and vertical dispersion parameters in the Pasquill-Gifford programs were modeled with simple, two-parameter functions that agreed very well with the usual textbook graphs. 8 references, 7 appendices.

  5. Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) Somaclones

    NASA Technical Reports Server (NTRS)

    Campbell, W. F.; Carman, J. G.; Hashim, Z. N.

    1990-01-01

    The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.

  6. Linear and nonlinear interpretation of CV-580 lightning data

    NASA Technical Reports Server (NTRS)

    Ng, Poh H.; Rudolph, Terence H.; Perala, Rodney A.

    1988-01-01

    Numerical models developed for the study of lightning strike data acquired by in-flight aircraft are applied to the data measured on the CV-580. The basic technique used is the three dimensional time domain finite difference solution of Maxwell's equations. Both linear and nonlinear models are used in the analysis. In the linear model, the lightning channel and the aircraft are assumed to form a linear time invariant system. A transfer function technique can then be used to study the response of the aircraft to a given lightning strike current. Conversely, the lightning current can be inferred from the measured response. In the nonlinear model, the conductivity of air in the vicinity of the aircraft is calculated and incorporated into the solution of the Maxwell's equations. The nonlinear model thus simulates corona formation and air breakdown. Results obtained from the models are in reasonable agreement with the measured data. This study provides another validation of the models and increases confidence that the models may be used to predict aircraft response to any general lightning strike.

  7. MIZEX-WEST NASA CV-990 flight report

    NASA Technical Reports Server (NTRS)

    Cavalieri, D. J.; Gloersen, P.

    1983-01-01

    As part of the Bering Sea marginal ice zone winter experiment (MIZEX-WEST), the CV-990 airborne laboratory was flown to assess the potential of using an extended range of wavelengths for improving passive microwave sea ice observations from spacecraft and second to provide an overview of the MIZ for large-scale processes studies. The aircraft was equipped with both imaging and fixed-beam, dual-polarized passive microwave radiometers ranging from 1.5 millimeter to 3 centimeter wavelengths. Visual, photographic, and thermal (10.7 micron) infrared surface observations were also made from the aircraft to complement the microwave measurements. The flight operations and in-flight observations are discussed and each flight is summarized including flight objective and instrument status. Preliminary mosaic images obtained with the ESMR imager, Nimbus-7 orbits over the Bering Sea, ice observations obtained by an ice observer on board, and composite maps of the general ice conditions for the month of February are also presented.

  8. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  9. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-05-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  10. Solution NMR Structure of Hypothetical Protein CV_2116 Encoded by a Viral Prophage Element in Chromobacterium violaceum

    SciTech Connect

    Yang, Yunhuang; Ramelot, Theresa A.; Cort, John R.; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl H.; Kennedy, Michael A.

    2012-06-14

    CV{_}2116 from Chromobacterium violaceum is a small hypothetical protein of 82 amino acids. A PSI-BLAST search using the CV{_}2116 protein sequence as a query identified only two hits, both with amino acid sequence identities of less than 40%. After the CV{_}2116 gene was cloned into the p15TvLic expression plasmid and transformed into E. coli, the desired CV{_}2116 protein was expressed and purified. A high quality solution structure of CV{_}2116 was determined by NMR spectroscopy. The tertiary structure of CV{_}2116 adopts a novel alpha + beta fold containing two anti-parallel beta sheets and one alpha helix in the C-terminal end. CV{_}2116 does not belong to any known protein sequence families and no similar structures exist in the protein data bank. To date, no function of CV{_}2116 can be derived from either sequence or structural similarity searches.

  11. Influence of Lachancea thermotolerans on cv. Emir wine fermentation.

    PubMed

    Balikci, Eren Kemal; Tanguler, Hasan; Jolly, Neil P; Erten, Huseyin

    2016-07-01

    The present paper describes the behaviour of Lachancea thermotolerans and Saccharomyces cerevisiae in pure, co-cultured and sequential fermentations in cv. Emir grape must. Faster fermentation rates were observed in wine made with a pure culture of S. cerevisiae and wine produced with simultaneously inoculated cultures of L. thermotolerans and S. cerevisiae. Both L. thermotolerans and S. cerevisiae gave high population numbers. The use of L. thermotolerans in mixed and sequential cultures led to an increase in final total acidity content in the wines, varying in the range 5.40-6.28 g/l (as tartaric acid), compared to pure culture S. cerevisiae, which gave the lowest level of total acidity (5 g/l). The increase was in the order of 1.18-2.06 g/l total acidity. Increase in final acidity by the use of L. thermotolerans might be useful to improve wines with low acidity due to global climate change. Volatile acidity levels (as acetic acid) were in the range 0.53-0.73 g/l, while the concentration of ethyl alcohol varied in the range 10.76-11.62% v/v. Sequential fermentations of wines and pure culture fermentation of L. thermotolerans resulted in reduction in the concentrations of acetaldehyde and higher alcohols, with exception of N-propanol and esters. According to the sensory analysis, wine obtained with sequential inoculation of L. thermotolerans followed by inoculation of S. cerevisiae after 24 h, and simultaneous inoculation of these yeasts, was the most preferred. Copyright © 2016 John Wiley & Sons, Ltd. PMID:27113383

  12. Self-assembling of impurity clusters in AlN:(Ga, BV, CV), (BV, CV = P, As; P, Sb; As, Sb)

    NASA Astrophysics Data System (ADS)

    Elyukhin, V. A.

    2015-11-01

    The self-assembling conditions of arrays of tetrahedral impurity clusters of two types in zinc blende AlN:(Ga, BV, CV), (BV, CV = P, As; P, Sb; As, Sb) are represented. Doping with one cation and two anion isoelectronic impurities transforms AlN into AlN-rich GaxAl1-xBVyCVzN1-y-z alloy of GaBV, GaCV, GaN, AlBV, AlCV and AlN. The cause of self-assembling is the preference of GaBV, GaCV and AlN bonding over that of GaN, AlBV, AlCV. The conditions are considered from 0 °C to 1000 °C in the dilute and ultra dilute limits for the cation and anion impurities, correspondingly. The temperature ranges between the cluster occurrence and self-assembling completion when the same anion impurities are in clusters are very small. 1P4Ga and 1As4Ga cluster occurrence temperatures are equal, correspondingly, to 797 °C and 736 °C at Ga content 2% and P and As contents 0.01%. 1P4Ga and 1Sb4Ga cluster occurrence temperatures are equal, correspondingly, to 976 °C and 736 °C at the same impurity contents. The cluster densities in AlN:(Ga, As, Sb) are close to those in AlN:(Ga, P, Sb). The results demonstrate that studied semiconductors are promising materials to produce arrays of identical ∼1 nm low band gap objects of two types embedded in the wide band gap matrix.

  13. Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV)

    SciTech Connect

    Shi Min; Chen Yafeng; Huang Fang; Liu Pengcheng; Zhou Xueping; Chen Xuexin

    2008-06-05

    Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with I{kappa}B-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy.

  14. Visualization of HDF/HDF-EOS Format Earth Observing System Data Using the ISIS "cv" Program

    NASA Astrophysics Data System (ADS)

    Torson, J. M.

    2001-05-01

    The "cv" (Cube Visualization) program has been used for a number of years as part of the ISIS image processing system (Integrated Software for Imagers and Spectrometers) [1],[2]. In addition to reading the native ISIS image cube format, "cv" has recently been enhanced to directly read the HDF and HDF-EOS file formats used for distributing data from various Earth Observing System (EOS) Missions (e.g. the Terra and Landsat-7 satellites). Files based on HDF Version 4.x are supported; files based on the new HDF 5.x are not yet supported. "cv" is now packaged so that it can be used without installing the rest of the ISIS software system. The capabilities of the program include: Displays (as images) any Swath/Grid data fields in HDF-EOS files; Displays (as images) any Scientific Data Set (SDS) data fields in HDF files; Combines multiple HDF/HDF-EOS fields to form one display object; Subarea selection and/or subsampling (allows handling large files); Simultaneous display of multiple images/files; Plots intensity profiles along any of the three axes in a 3D data set; Writes displayed data fields to binary files (allows doing further processing using ISIS programs or using other software packages); Reports cursor location and pixel value (includes reporting Latitude/Longitude with optional conversion between geocentric and geographic coordinates); Includes many additional flexible display options. The "cv" program is implemented in the IDL language and makes use of the IDL CALL_EXTERNAL capability to call I/O and utility routines written in C and Fortran. Pre-compiled versions of "cv" are available for Sun Solaris, Compaq Alpha and PC Linux platforms. To obtain "cv", go to the USGS anonymous ftp site (ftpflag.wr.usgs.gov). Do a "cd dist/isis" and get (in binary mode) the README_CV.TXT file (installation instructions) and the tar file for the desired platform (cv_sun.tar, cv_alpha.tar, cv_pc.tar). More information on ISIS is available at the ISIS website (http

  15. Circulating HFMD-Associated Coxsackievirus A16 Is Genetically and Phenotypically Distinct from the Prototype CV-A16

    PubMed Central

    Li, Jingliang; Ren, Sangsang; Wei, Zhenhong; Bao, Wanguo; Hu, Xiaoming; Zhao, Ke; Zhang, Wenyan; Zhou, Yulai; Sun, Fei; Markham, Richard; Yu, Xiao-Fang

    2014-01-01

    Human enteroviruses (HEV) have been linked to hand, foot, and mouth disease (HFMD) in the Pacific and Southeast Asia for decades. Many cases of HFMD have been attributed to coxsackievirus A16 (CV-A16, CA16), based on only partial viral genome determination. Viral phenotypes are also poorly defined. Herein, we have genetically and phenotypically characterized multiple circulating CV-A16 viruses from HFMD patients and determined multiple full-length sequences of these circulating viruses. We discovered that the circulating CV-A16 viruses from HFMD patients are genetically distinct from the proto-type CV-A16 G10. We have also isolated circulating CV-A16 viruses from hospitalized HFMD patients and compared their virological differences. Interestingly, circulating CV-A16 viruses are more pathogenic in a neonatal mouse model than is CV-A16 G10. Thus, we have found circulating recombinant forms of CV-A16 (CRF CV-A16) that are related to, but different from, the prototype CV-A16 G10 that have distinct biological phenotypes. PMID:24736564

  16. Non-contact C-V measurements of ultra thin dielectrics

    NASA Astrophysics Data System (ADS)

    Edelman, P.; Savtchouk, A.; Wilson, M.; D'Amico, J.; Kochey, J. N.; Marinskiy, D.; Lagowski, J.

    2004-07-01

    In this paper, we present a non-contact C-V technique for ultra-thin dielectrics on silicon. The technique uses incremental corona charging of dielectric and a measurement of the surface potential with a vibrating capacitive electrode. A differential quasistatic C-V curve is generated using time-resolved measurements. The technique incorporates transconductance corrections that enable corresponding ultra-low electrical oxide thickness (EOT) determination down to the sub-nanometer range. It also provides a means for monitoring the flat band voltage, V{FB}, the interface trap spectrum, D{IT}, and the total dielectric charge, Q{TOT}. This technique is seen as a replacement for not only MOS C-V measurements but also for mercury-probe C-V. In addition, EOT measurement by the corona C-V has a major advantage over optical thickness methods because it is not affected by water adsorption and molecular airborne contamination, MAC. These effects have been a problem for optical metrology of ultra-thin dielectrics.

  17. The Ningqiang meteorite - Classification and petrology of an anomalous CV chondrite

    NASA Astrophysics Data System (ADS)

    Rubin, A. E.; Wang, D.; Kallemeyn, G. W.; Wasson, J. T.

    1988-03-01

    Ningqiang is an anomalous CV chondrite (oxidized subgroup) containing a high abundance of aggregational inclusions (13.7 vol pct) and low abundances of refractory inclusions (1.0 + 1.0 or - 0.5 vol pct) and bulk refractory lithophiles (about 0.82 x CV). Ningqiang may have agglomerated after most refractory inclusions at the nebular midplane had already been incorporated into other objects. Coarse-grained rims surround only about 5 percent of Ningqiang chondrules, compared to about 50 percent in normal CV chondrites. Aggregational inclusions appear to have formed by incipient melting of fine-grained aggregates at relatively low temperatures in the solar nebula, possibly by the mechanism responsible for chondrule formation.

  18. Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.

    PubMed

    Matsushima, Kazuki; Kaneda, Hirokazu; Harada, Kazuo; Matsuura, Hideyuki; Hirata, Kazumasa

    2015-05-01

    Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater. PMID:25700813

  19. CV-990 Landing Systems Research Aircraft (LSRA) during final Space Shuttle tire test

    NASA Technical Reports Server (NTRS)

    1995-01-01

    A Convair 990 (CV-990) was used as a Landing Systems Research Aircraft (LSRA) at NASA's Dryden Flight Research Center, Edwards, California, to test space shuttle landing gear and braking systems as part of NASA's effort to upgrade and improve space shuttle capabilities. The first flight at Dryden of the CV-990 with shuttle test components occurred in April 1993, and tests continued into August 1995, when this photo shows a test of the shuttle tires. The purpose of this series of tests was to determine the performance parameters and failure limits of the tires. This particular landing was on the dry lakebed at Edwards, but other tests occurred on the main runway there. The CV-990, built in 1962 by the Convair Division of General Dynamics Corp., Ft. Worth, Texas, served as a research aircraft at Ames Research Center, Moffett Field, California, before it came to Dryden.

  20. Segmentation of kidney using C-V model and anatomy priors

    NASA Astrophysics Data System (ADS)

    Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

    2007-12-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

  1. The dichotic lead effect of CV syllables in sensorineural hearing loss.

    PubMed

    Cattey, T J

    1981-01-01

    This study investigated central processing of dichotic CV syllables on 10 patients with sensorineural hearing losses. Porter's Dichotic Lag Index (DLI) was established for each S at asynchronies of alignment of 30, 60, 90, and 150 msec by presenting the lagging syllable first to the R and then to the L ear. Ss were required to record a response to both CV syllables. Patients exhibited a dichotic lead advantage while 10 normal-hearing control Ss exhibited a dichotic lag advantage. A R ear advantage was not abolished when the lagging syllable was presented to the L ear and was not enhanced when the lagging syllable was presented to the R ear. The lead advantage of the patients may be attributed to the distortion of acoustic encoding for speech, causing a reduction in the special processing requirements of CV syllables. The reduction in special processing may then be responsible for the shift in temporal advantage. PMID:7349869

  2. Mineralogy and Petrography of MIL 090001, a Highly Altered CV Chondrite from the Reduced Sub-Group

    NASA Technical Reports Server (NTRS)

    Keller, Lindsay P.

    2011-01-01

    MIL 090001 is a large (greater than 6 kg) CV chondrite from the reduced subgroup (CV(sub red)) that was recovered during the 2009-2010 ANSMET field season [1]. The CV(sub red) subgroup meteorites retain primitive characteristics and have escaped the Na and Fe meta-somatism that affected the oxidized (CV(sub ox)) subgroups. MIL 090001 is, however, reported to be altered [1], and thus a major objective of this study is to characterize its mineralogy and petrography and the extent of the alteration.

  3. Study of Antimicrobial and Cytotoxic Potential of the Oyster Mushroom Pleurotus ostreatus cv. Florida (Agaricomycetes).

    PubMed

    Ganeshpurkar, Aditya; Bhadoriya, Santosh Singh; Pardhi, Priya; Jain, Alok Pal; Rai, Gopal

    2016-01-01

    This work was undertaken to evaluate in vitro antimicrobial and cytotoxic potential of Pleurotus ostreatus cv. Florida. Mushroom basidiocarps were extracted in water:ethanol (1:1, v/v), and the resulting extract was subjected to antimicrobial studies against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella oxytoca, Bacillus subtilis, and Candida albicans. Cytotoxic potential on viable human leukocytes was studied. In vitro results showed excellent antimicrobial and cytotoxic potentials of the mushroom extract. Thus, functional properties of P. ostreatus cv. Florida could be used in the search for novel therapeutics. PMID:27481298

  4. Presolar Grain Inventories of the Ungrouped C3 Adelaide and the CV3 RBT 04133

    NASA Astrophysics Data System (ADS)

    Davidson, J.; Busemann, H.; Franchi, I. A.; Grady, M. M.

    2010-03-01

    Here we report the presolar grain inventories (silicates, oxides, SiC, and other C-anomalous phases) determined for Adelaide (an ungrouped C3 chondrite) and RBT 04133 (a mildly thermally altered CV3) by NanoSIMS raster ion imaging.

  5. Outburst of CV ROTSE3 J031031.4+431115.0

    NASA Astrophysics Data System (ADS)

    Dhungana, G.; Ferrante, F. V.; Staten, R.; Kehoe, R.

    2015-02-01

    Further to ATel#1272, we report observations of an outburst of the U Geminorum-type CV ROTSE3 J031031.4+431115.0 in unfiltered CCD images taken by the 0.45 m ROTSE-IIIb telescope at McDonald Observatory, Texas.

  6. Some Improvements in the Design of a CA/CV Moessbauer Velocity Drive

    SciTech Connect

    Seberini, Milan

    2008-10-28

    A constant velocity Moessbauer drive was built with velocity range {+-}15 mm/s and velocity resolution 0.005 mm/s. Based on good experience with its performance, a new universal CA/CV drive was designed. The new drive is supposed to have velocity range of {+-}80 mm/s with a velocity resolution below 0.002 mm/s.

  7. Petrological Investigations of CAIs from Efremovka and NWA 3118 CV3 Chondrites

    NASA Astrophysics Data System (ADS)

    Ivanova, M. A.; Lorenz, C. A.; Korochantseva, E. V.; MacPherson, G. J.

    2010-03-01

    Several new big CAIs were extracted from the Efremovka and NWA 3118 CV3 chondrites to analyze petrology, chemistry and isotopic compositions. Here we report preliminary results on mineralogy, petrology and bulk chemistry of two CAIs, of Type B1 and of Type A.

  8. Indirect measurements of Brachiaria brizantha cv. Marandu fermentable cell wall sugars for second generation biofuels production.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Results of a study conducted to evaluate the possibility of using IVDMD values of B. brizantha cv. Marandu to predict cell wall sugars that would be available in a biorefinery for ethanol production are reported. The study was conducted based on the similarity between rumen enzymes and those used i...

  9. Identification of HoCV-1 virus in Texas Glassy-Winged Sharpshooter Populations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A new strain of leafhopper virus was discovered in leafhoppers which are vectors of Pierce’s disease of grapes. The original leafhopper infecting virus, HoCV-1, was isolated from the glassy-winged sharpshooter, Homalodisca vitripennis, from samples collected in California and Florida. Using the orig...

  10. Abstract Morphemes and Lexical Representation: The CV-Skeleton in Arabic

    ERIC Educational Resources Information Center

    Boudelaa, Sami; Marslen-Wilson, Willian D.

    2004-01-01

    Overlaps in form and meaning between morphologically related words have led to ambiguities in interpreting priming effects in studies of lexical organization. In Semitic languages like Arabic, however, linguistic analysis proposes that one of the three component morphemes of a surface word is the CV-Skeleton, an abstract prosodic unit coding the…

  11. 75 FR 54887 - Determination of Regulatory Review Period for Purposes of Patent Extension; REPEL-CV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-09

    ..., for the extension of a patent which claims that medical device. ADDRESSES: Submit electronic comments... 17, 2010, FDA advised the Patent and Trademark Office that this medical device had undergone a... Patent Extension; REPEL-CV AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The...

  12. Pre-Accretionary Distribution of Ca and Al Between Matrix and Chondrules in CV Chondrites

    NASA Astrophysics Data System (ADS)

    Hezel, D. C.; Palme, H.

    2007-03-01

    Ca/Al-ratios in Y-86751 (CV) chondrules are super- and in matrix sub-chondritic. The opposite is true for Allende and Efremovka. Incorporation of spinel in Allende and Efremovka chondrule precursors in a nebular setting can explain this observation.

  13. Thermal degradation of anthocyanins from purple potato (Cv. Purple Majesty) and their impact on antioxidant capacity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Degradation parameters of purified anthocyanins from purple-fleshed potato (Purple Majesty cv.) heated at high temperatures (100 - 150 °C) was determined. Purified anthocyanins, prepared by removing salts, sugars and colorless non-anthocyanin phenolics from the crude extract, were quantified using H...

  14. VizieR Online Data Catalog: 72 faint CV candidates in CRTS (Breedt+, 2014)

    NASA Astrophysics Data System (ADS)

    Breedt, E.; Gansicke, B. T.; Drake, A. J.; Rodriguez-Gil, P.; Parsons, S. G.; Marsh, T. R.; Szkody, P.; Schreiber, M. R.; Djorgovski, S. G.

    2016-04-01

    We obtained identification spectra of a total of 72 faint CV candidates identified by the CRTS, using the Gran Telescopio Canarias (GTC; La Palma, Spain) and the Gemini telescopes (North: Mauna Kea, Hawaii and South: Cerro Pachon, Chile). The observations were carried out in service mode during 2010, 2011 and 2013. (5 data files).

  15. Optimized growth and plant regeneration for callus of Lilium longiflorum cv. Nellie White

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The rates of growth and regeneration were compared for compact callus, friable callus, and suspension cells of Lilium longiflorum cv. Nellie White to determine the optimal culture conditions. The fresh weight was higher for compact callus induced from bulb scales cultured on Murashige and Skoog’s m...

  16. Automatic tracking of red blood cells in micro channels using OpenCV

    NASA Astrophysics Data System (ADS)

    Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

    2013-10-01

    The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

  17. Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The distribution of a potential biological agent, the newly discovered leafhopper-infecting virus, HoCV-1 was examined across the USA. Few biological agents exist for use against leafhopper pests. We examined and compared leafhopper salivary gland and midgut tissues using transmission electron micro...

  18. Safe, Effective and Easily Reproducible Fusion Technique for CV Junction Instability

    PubMed Central

    Sannegowda, Raghavendra Bakki

    2015-01-01

    Introduction: The Craniovertebral junction (CVJ) refers to a bony enclosure where the occipital bone surrounds the foramen magnum, the atlas and the axis vertebrae. Because of the complexity of structures, CVJ instability is associated with diagnostic and therapeutic problems. Posterior CV fusion procedures have evolved a lot over the last couple of decades. There has been a lookout for one such surgical procedure which is inherently safe, simple, easily reproducible and biomechanically sound. In our study, we present the initial experience the cases of CV junction instrumentation using O-C1-C2 screw & rod construct operated by the author. Aims and Objectives: The current study is a descriptive analysis of the cases of CVJ instability treated by us with instrumentation using O-C1-C2 screw and rod construct fusion technique. Materials and Methods: It is a retrospective, analytical study in which cases of CV junction instability operated by the author between January 2010 to March 2014 were analysed using various clinical, radiological and outcome parameters. Conclusion: CV junction instrumentation using O-C1-C2 screw and rod construct fusion technique proved to be safe, effective, easily reproducible and biomechanically sound technique which can be adopted by all surgeons who may be at any stage of their learning curve. PMID:25954660

  19. Isolation and Characterization of CvIV4: A Pain Inducing α- Scorpion Toxin

    PubMed Central

    Rowe, Ashlee H.; Xiao, Yucheng; Scales, Joseph; Linse, Klaus D.; Rowe, Matthew P.; Cummins, Theodore R.; Zakon, Harold H.

    2011-01-01

    Background Among scorpion species, the Buthidae produce the most deadly and painful venoms. However, little is known regarding the venom components that cause pain and their mechanism of action. Using a paw-licking assay (Mus musculus), this study compared the pain-inducing capabilities of venoms from two species of New World scorpion (Centruroides vittatus, C. exilicauda) belonging to the neurotoxin-producing family Buthidae with one species of non-neurotoxin producing scorpion (Vaejovis spinigerus) in the family Vaejovidae. A pain-inducing α-toxin (CvIV4) was isolated from the venom of C. vittatus and tested on five Na+ channel isoforms. Principal Findings C. vittatus and C. exilicauda venoms produced significantly more paw licking in Mus than V. spinigerus venom. CvIV4 produced paw licking in Mus equivalent to the effects of whole venom. CvIV4 slowed the fast inactivation of Nav1.7, a Na+ channel expressed in peripheral pain-pathway neurons (nociceptors), but did not affect the Nav1.8-based sodium currents of these neurons. CvIV4 also slowed the fast inactivation of Nav1.2, Nav1.3 and Nav1.4. The effects of CvIV4 are similar to Old World α-toxins that target Nav1.7 (AahII, BmK MI, LqhIII, OD1), however the primary structure of CvIV4 is not similar to these toxins. Mutant Nav1.7 channels (D1586A and E1589Q, DIV S3–S4 linker) reduced but did not abolish the effects of CvIV4. Conclusions This study: 1) agrees with anecdotal evidence suggesting that buthid venom is significantly more painful than non-neurotoxic venom; 2) demonstrates that New World buthids inflict painful stings via toxins that modulate Na+ channels expressed in nociceptors; 3) reveals that Old and New World buthids employ similar mechanisms to produce pain. Old and New World α-toxins that target Nav1.7 have diverged in sequence, but the activity of these toxins is similar. Pain-inducing toxins may have evolved in a common ancestor. Alternatively, these toxins may be the product of convergent

  20. Formation timescales of CV chondrites from component specific Hf-W systematics

    NASA Astrophysics Data System (ADS)

    Becker, Maike; Hezel, Dominik C.; Schulz, Toni; Elfers, Bo-Magnus; Münker, Carsten

    2015-12-01

    Carbonaceous chondrites are an important meteorite group that closely resembles the bulk composition of the solar system. We report the first elemental and isotope dataset for Hf-W in carbonaceous chondrites that includes chondrules, matrix, magnetic fractions as well as bulk compositions. Our study focuses on the three CV3 chondrites, Allende, Vigarano and Bali. Compared to bulk chondrites, matrix splits have low Hf/W ratios and ε182W compositions, whereas chondrule splits are characterized by high, but more variable, Hf/W ratios and ε182W compositions. Thus, Hf/W ratios behave complementary between chondrules and matrix in the analysed CV chondrites, supporting the view that both components formed from the same parental reservoir. Strong nucleosynthetic effects were observed in most of the analysed CV3 components, especially in matrices and chondrule splits that were found to have large ε183W anomalies of several ε-units. All separates define a rough correlation between initial 182W/184W and 183W/184W ratios, in agreement with theoretical model trends based on calculations for stellar nucleosynthesis. Our results, therefore, indicate a heterogeneous distribution of s- and r-process W isotopes among the different CV3 chondrite components, arguing for selective thermal processing of early solar system matter during chondrule formation. After correcting for nucleosynthetic anomalies, chondrules and matrix splits of reduced (Vigarano) as well as oxidised (Allende) CV3 chondrites define a linear correlation in ε182W vs. 180Hf/184W space, which is interpreted as an isochron, covering an age interval within the first ∼2.6 Ma after solar system formation. As peak metamorphic temperatures for CV3 chondrites were well below the 182Hf-182W closure temperature, the resulting isochron within its error most likely defines a common formation interval for all components. The calculated age interval is for the first time based on a combined chondrule-matrix isochron, a

  1. Modal mineralogy of CV3 chondrites by X-ray diffraction (PSD-XRD)

    NASA Astrophysics Data System (ADS)

    Howard, K. T.; Benedix, G. K.; Bland, P. A.; Cressey, G.

    2010-09-01

    Using position sensitive detector X-ray diffraction (PSD-XRD) we determine a complete modal mineralogy for all phases present in abundances greater than 1 wt% in Vigarano, Efremovka, Mokoia, Grosnaja, Kaba and Allende. Reduced CV3 samples are comprised of (vol%): olivine (83-85%); enstatite (6.5-8.1%); anorthite (1.1-1.2%); magnetite (1.4-1.8%); sulphide (2.4-5.1%); Fe, Ni metal (2-2.2%). The oxidized samples are comprised of: olivine (76.3-83.9%); enstatite (4.8-7.8%); anorthite (1.1-1.7%); magnetite (0.3-6.1%); sulphide (2.9-8.1%); Fe, Ni metal (0.2-1.1%); Fe-oxide (0-2.7%) and phyllosilicate (1.9-4.2%). When our modal data is used to calculate a bulk chemistry that is compared to literature data a near 1:1 correlation is observed. PSD-XRD data indicates that olivine compositions may span almost the entire Fe-Mg solid solution series in all CV samples and that these contain a component (4-13%) of fine-grained olivine that is more Fe-rich (>Fa 60) than is typically reported. Modal mineralogy shows that there are mineralogic differences between CV3 samples classified as oxidized and reduced but that these sub-classes are most clearly distinguished by the relative abundance of metal and Ni content of sulphide, rather than abundance of magnetite. The most significant difference in modal mineralogy observed is the relative absence of phyllosilicate in reduced CV that essentially escaped aqueous alteration. Fayalite, ferrous olivine and magnetite are typically considered secondary alteration products. The abundances of these minerals overlap in oxidized and reduced samples and correlate positively supporting common conditions of formation in a relatively oxidizing environment. The abundances of fayalite, ferrous olivine and magnetite show no relationship to petrographic type and if these abundances were used as a proxy for alteration, Allende would be the least altered CV - contrary to all previous data. The implication is that thermal metamorphism on the parent body

  2. Formation of biphenyl and dibenzofuran phytoalexins in the transition zones of fire blight-infected stems of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference'.

    PubMed

    Chizzali, Cornelia; Khalil, Mohammed N A; Beuerle, Till; Schuehly, Wolfgang; Richter, Klaus; Flachowsky, Henryk; Peil, Andreas; Hanke, Magda-Viola; Liu, Benye; Beerhues, Ludger

    2012-05-01

    In the rosaceous subtribe Pyrinae (formerly subfamily Maloideae), pathogen attack leads to formation of biphenyls and dibenzofurans. Accumulation of these phytoalexins was studied in greenhouse-grown grafted shoots of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference' after inoculation with the fire blight bacterium, Erwinia amylovora. No phytoalexins were found in leaves. However, both classes of defence compounds were detected in the transition zone of stems. The flanking stem segments above and below this zone, which were necrotic and healthy, respectively, were devoid of detectable phytoalexins. The transition zone of apple stems contained the biphenyls 3-hydroxy-5-methoxyaucuparin, aucuparin, noraucuparin and 2'-hydroxyaucuparin and the dibenzofurans eriobofuran and noreriobofuran. In pear, aucuparin, 2'-hydroxyaucuparin, noreriobofuran and in addition 3,4,5-trimethoxybiphenyl were detected. The total phytoalexin content in the transition zone of pear was 25 times lower than that in apple. Leaves and stems of mock-inoculated apple and pear shoots lacked phytoalexins. A number of biphenyls and dibenzofurans were tested for their in vitro antibacterial activity against some Erwinia amylovora strains. The most efficient compound was 3,5-dihydroxybiphenyl (MIC=115 μg/ml), the immediate product of biphenyl synthase which initiates phytoalexin biosynthesis. PMID:22377689

  3. GC-FID/MS Profiling of Supercritical CO2 Extracts of Peels from Citrus aurantium, C. sinensis cv. Washington navel, C. sinensis cv. Tarocco and C. sinensis cv. Doppio Sanguigno from Dubrovnik Area (Croatia).

    PubMed

    Jerković, Igor; Drulžić, Jasmina; Marijanović, Zvonimir; Gugić, Mirko; Jokić, Stela; Roje, Marin

    2015-07-01

    The peels of Citrus aurantium L. and Citrus sinensis Osbeck cultivars from the Dubrovnik region (south Croatia) were extracted by supercritical CO2 at 40 degrees C and 10 MPa at 1.76 kg/h to obtain enriched extracts in comparison with simple pressing of the peels. The extracts were analyzed in detail by gas chromatography and mass spectrometry (GC-FID/MS). Relevant similarities among the peel oil compositions of C. aurantium and C. sinensis cultivars were found with limonene predominance (up to 54.3%). The principal oxygenated monoterpenes were linalool (3.0%-5.9%), α-terpineol (0.7%-2.4%), linalyl acetate (0.0%-5.0%), geranyl acetate (0.0%-0.4%), (Z)-citral (0.0%-1.8%) and (E)-citral (0.0%-1.9%). Several sesquiterpenes were found with minor percentages. Coumarin derivatives were identified in all the samples among the relevant compounds. Isogeijerin dominated in the peels of C. sinensis cv. Tarocco (15.3%) and C. aurantium (11.2%). Scoparone ranged from 0.1% to 0.5% in all the samples. Bergapten (up to 1.4%), osthole (up to 1.1%) and 7-methoxy-8-(2-formylpropyl)coumarin (up to 1.1%) were found mostly in C. sinensis cv. Doppio Sanguigno. It was possible to indicate a few other differences among the extracts such as higher percentage of linalool, linalyl and geranyl acetates, as well as the abundance of sabinene and isogeijerin in C. aurantium or the occurrence of β-sinensal in C. sinensis cultivars. PMID:26411039

  4. The control volume radial basis function method CV-RBF with Richardson extrapolation in geochemical problems

    NASA Astrophysics Data System (ADS)

    Florez, W. F.; Portapila, M.; Hill, A. F.; Power, H.; Orsini, P.; Bustamante, C. A.

    2015-03-01

    The aim of this paper is to present how to implement a control volume approach improved by Hermite radial basis functions (CV-RBF) for geochemical problems. A multi-step strategy based on Richardson extrapolation is proposed as an alternative to the conventional dual step sequential non-iterative approach (SNIA) for coupling the transport equations with the chemical model. Additionally, this paper illustrates how to use PHREEQC to add geochemical reaction capabilities to CV-RBF transport methods. Several problems with different degrees of complexity were solved including cases of cation exchange, dissolution, dissociation, equilibrium and kinetics at different rates for mineral species. The results show that the solution and strategies presented here are effective and in good agreement with other methods presented in the literature for the same cases.

  5. Xoconostle fruit (Opuntia matudae Scheinvar cv. Rosa) by-products as potential functional ingredients.

    PubMed

    Morales, Patricia; Barros, Lillian; Ramírez-Moreno, Esther; Santos-Buelga, Celestino; Ferreira, Isabel C F R

    2015-10-15

    There is a lack of information on the potential use of xoconostle cultivars as sources of antioxidants for food, pharmaceutical and colorant industries. The aim of this study was to provide a phytochemical characterisation and antioxidant activity evaluation of Opuntia matudae Scheinvar cv. Rosa by-products (epicarp and endocarp mucilage's), in order to evaluate their interest as sources of functional ingredients for human or animal foods. These by-products showed a high content in glucose, citric and linoleic acids, tocopherols, and isorhamnetin-O-(di-deoxyhexosyl-hexoside) (mainly in epicarp), and presented relevant antioxidant properties. The obtained results support the use of O. matudae Scheinvar cv. Rosa agro-industrial by-products as functional food ingredients, namely for antioxidant-enriched formulations, instead of being discarded. PMID:25952871

  6. A model for the C-V characteristics of the metal-ferroelectric-insulator-semiconductor structure

    NASA Astrophysics Data System (ADS)

    Zhang, Jun Jie; Sun, Jing; Zheng, Xue Jun

    2009-02-01

    A model is developed to describe the characteristics of the metal-ferroelectric-insulator-semiconductor (MFIS) structure based on the dipole switching theory (DST) and the silicon physics of metal-oxide-semiconductor (MOS) structure. The ferroelectric dipole distribution function is used to simulate the history-dependent electric field effect of the ferroelectric layer. Using the model, the thickness effects of the ferroelectric and insulator layers on the capacitance-voltage ( C-V) characteristic and the memory window were investigated for Pt/SBT/ZrO 2/Si and Pt/BLT/MgO/Si structures. All the simulation results show good agreement with the experimental results, indicating that the model is suitable for simulating the C-V characteristic and the memory window of MFIS structure. In addition, the mathematical description is simple and can be easily integrated into the electronic design automation (EDA) software for circuit simulation.

  7. Graphical method for determining the coefficient of consolidation cv from a flow-pump permeability test

    USGS Publications Warehouse

    Morin, Roger H.; Olsen, Harold W.; Nelson, Karl R.; Gill, James D.

    1989-01-01

    A graphical method has been developed for determining the coefficient of consolidation from the transient phases of a flow-pump permeability test. The flow pump can be used to infuse fluid into or withdraw fluid from a laboratory sediment specimen at a constant volumetric rate in order to obtain data that can be used to calculate permeability using Darcy's law. Representative type-curve solutions to the associated forced-flow and pressure-decay models are derived. These curves provide the basis for graphically evaluating the permeability k, the coefficient of consolidation cv, and the coefficient of volume change mv. The curve-matching technique is easy and rapid. Values of k, cv and mv for a laterally confined kaolinite specimen were determined by this graphical method and appear to be in reasonably good agreement with numerically derived estimates (within 20%). Discrepancies between the two sets of results seem to be largely a function of data quality.

  8. Camera calibration method of binocular stereo vision based on OpenCV

    NASA Astrophysics Data System (ADS)

    Zhong, Wanzhen; Dong, Xiaona

    2015-10-01

    Camera calibration, an important part of the binocular stereo vision research, is the essential foundation of 3D reconstruction of the spatial object. In this paper, the camera calibration method based on OpenCV (open source computer vision library) is submitted to make the process better as a result of obtaining higher precision and efficiency. First, the camera model in OpenCV and an algorithm of camera calibration are presented, especially considering the influence of camera lens radial distortion and decentering distortion. Then, camera calibration procedure is designed to compute those parameters of camera and calculate calibration errors. High-accurate profile extraction algorithm and a checkboard with 48 corners have also been used in this part. Finally, results of calibration program are presented, demonstrating the high efficiency and accuracy of the proposed approach. The results can reach the requirement of robot binocular stereo vision.

  9. Automatic segmentation of Leishmania parasite in microscopic images using a modified CV level set method

    NASA Astrophysics Data System (ADS)

    Farahi, Maria; Rabbani, Hossein; Talebi, Ardeshir; Sarrafzadeh, Omid; Ensafi, Shahab

    2015-12-01

    Visceral Leishmaniasis is a parasitic disease that affects liver, spleen and bone marrow. According to World Health Organization report, definitive diagnosis is possible just by direct observation of the Leishman body in the microscopic image taken from bone marrow samples. We utilize morphological and CV level set method to segment Leishman bodies in digital color microscopic images captured from bone marrow samples. Linear contrast stretching method is used for image enhancement and morphological method is applied to determine the parasite regions and wipe up unwanted objects. Modified global and local CV level set methods are proposed for segmentation and a shape based stopping factor is used to hasten the algorithm. Manual segmentation is considered as ground truth to evaluate the proposed method. This method is tested on 28 samples and achieved 10.90% mean of segmentation error for global model and 9.76% for local model.

  10. TST from geofinder traverse data on HP41CV programmable calculator

    SciTech Connect

    Carman, G.J. )

    1989-02-01

    Using program subroutines designed for the HP41CV programmable calculator and the geometric data recorded from Geofinder traversing or other pace-and-compass-type methods, the true stratigraphic thickness (TST) of dipping or gently folded strata is calculated at a rate of about 30 seconds per station. TST information is therefore readily available for reviewing stratigraphic and structure data and for post survey graphical plots.

  11. Comparison of direct mercury analyzer and FIA-CV-AAS in determination of methylmercury in fish

    NASA Astrophysics Data System (ADS)

    Ulrich, J. C.; Hortellani, M. A.; Sarkis, J. E. S.; Nakatsubo, M. A.

    2016-07-01

    Methylmercury (MeHg) has been determined in fish reference materials by direct mercury analyzer (DMA 80) and FIA-CV-AAS. In order to evaluate accuracy, certified reference materials (Fish protein, NRCC - Dorm 4 and fish material, Ipen - Dourada 1) were analyzed after extraction and separation of mercury species. Good agreement of the results have been obtained (relative error of the determination between the methods varied from 1.5% to 39%). The repeatability of the results varied from 4% to 26%.

  12. A real-time camera calibration system based on OpenCV

    NASA Astrophysics Data System (ADS)

    Zhang, Hui; Wang, Hua; Guo, Huinan; Ren, Long; Zhou, Zuofeng

    2015-07-01

    Camera calibration is one of the essential steps in the computer vision research. This paper describes a real-time OpenCV based camera calibration system, and developed and implemented in the VS2008 environment. Experimental results prove that the system to achieve a simple and fast camera calibration, compared with MATLAB, higher precision and does not need manual intervention, and can be widely used in various computer vision system.

  13. IV and CV curves for irradiated prototype BTeV silicon pixel sensors

    SciTech Connect

    Maria R. Coluccia et al.

    2002-07-16

    The authors present IV and CV curves for irradiated prototype n{sup +}/n/p{sup +} silicon pixel sensors, intended for use in the BTeV experiment at Fermilab. They tested pixel sensors from various vendors and with two pixel isolation layouts: p-stop and p-spray. Results are based on exposure with 200 MeV protons up to 6 x 10{sup 14} protons/cm{sup 2}.

  14. Isolation of an immunosuppressive lectin from Phaseolus vulgaris L. cv Cacahuate using stroma.

    PubMed

    Vargas-Albores, F; Hernández, J; Córdoba, F; Zenteno, E

    1993-11-01

    An immunosuppressive lectin was isolated from seed of Phaseolus vulgaris cv Cacahuate using physically entrapped stroma. The lectin was found to be a 94 kDa tetrameric protein. When 50 micrograms, of this lectin were administered intraperitoneally 2 days before the immunization with sheep red blood cells, humoral response against the immunogen was completely inhibited. Other properties of the protein are discussed. PMID:8248029

  15. CV population densities: Using CFHT as the worlds largest time-series photometer

    NASA Astrophysics Data System (ADS)

    Lott, D. A.; Haswell, C. A.; Abbott, T. M. C.; Ringwald, F.

    2002-01-01

    Known samples of cataclysmic variables (CVs) are sparse and/or rife with selection effects and do not provide adequate tests of theories of their evolution. We have used the CFHT12k mosaic camera as the worlds largest time-series photometer, observing a 0.3 sq.deg. Galactic field with 3 minute time-resolution. The resulting unbiased, magnitude-limited sample of CVs, and their progenitors will be used to test the predictions of CV population models.

  16. Anthelmintic activities of aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana.

    PubMed

    Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

    2014-01-01

    Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana. PMID:24583851

  17. Modelling the Galactic CV distribution for the ChaMPlane Project

    NASA Astrophysics Data System (ADS)

    Rogel, A. B.; Lugger, P. M.; Cohn, H. N.

    2004-12-01

    We model the cataclysmic variable (CV) distribution in the Galaxy to better understand CV detection rates for the ChaMPlane survey of accreting X-ray sources in the Galactic plane. The CV distribution is modeled as a warped, flared exponential disk with a Gaussian vertical structure. Extinction is based on the recently published Galactic dust and gas model by Drimmel, et al. A luminosity function for CVs is also incorporated, based on a smoothed version of published data by Patterson. We use Monte-Carlo techniques to distribute of order 1-2 × 106 CVs throughout the Galaxy. We then determine the expected number per NOAO 4m telescope Mosaic field as a function of limiting magnitude and Galactic coordinates, within the plane but outside of the bulge (20o

  18. Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

    PubMed Central

    Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

    2014-01-01

    Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (−)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana. PMID:24583851

  19. Utrophins compensate for Dp71 absence in mdx3cv in adhered platelets.

    PubMed

    Cerecedo, Doris; Mondragón, Ricardo; Candelario, Aurora; García-Sierra, Francisco; Mornet, Dominique; Rendón, Alvaro; Martínez-Rojas, Dalila

    2008-01-01

    Platelet adhesion is a critical step due to its hemostatic role in stopping bleeding after vascular damage. Short dystrophins are the most abundant dmd gene products in nonmuscle tissues, and in association with cytoskeleton proteins contribute to their intrinsic function; while utrophins are dystrophin-homologous related family proteins with structural and functional similarities. We previously demonstrated the presence of Dp71 isoforms, utrophins, and various dystrophin-associated proteins and their participation in cytoskeleton re-organization, filopodia and lamellipodia extension, and in centralizing cytoplasmic granules during the adhesion process of human platelets. To evaluate the morphologic changes and actin-based structures of mdx(3cv) platelets during the adhesion process, we compared the topographic distribution of Dp71d/Dp71Delta110(m) and dystrophin-associated protein in adhered platelets from dystrophic mdx(3cv) mouse. By confocal microscopy, we showed that absence of Dp71 isoforms in platelets from this animal model disrupted dystrophin-associated protein expression and distribution without modifying the platelet morphology displayed during the glass-adhesion process. By immunoprecipitation assays, we proved that up-regulated utrophins were associated with dystrophin-associated proteins to conform the dystrophin-associated protein complex corresponding to utrophins, which might compensate for Dp71 absence in mdx(3cv) platelets. PMID:18180614

  20. Teaching image processing and pattern recognition with the Intel OpenCV library

    NASA Astrophysics Data System (ADS)

    Kozłowski, Adam; Królak, Aleksandra

    2009-06-01

    In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image processing and pattern recognition with the most up-to-date solutions available. In the Institute of Electronics at the Technical University of Lodz we facilitate the teaching process in this subject with the OpenCV library, which is an open-source set of classes, functions and procedures that can be used in programming efficient and innovative algorithms for various purposes. The topics of student projects completed with the help of the OpenCV library range from automatic correction of image quality parameters or creation of panoramic images from video to pedestrian tracking in surveillance camera video sequences or head-movement-based mouse cursor control for the motorically impaired.

  1. Secondary Mineralization of Components in CV3 Chondrites: Nebular and Asteroidal Models

    NASA Astrophysics Data System (ADS)

    Scott, E. R. D.; Krot, A. N.; Zolensky, M. E.

    1995-09-01

    Our review of mineralogical variations among CV3 chondrites suggests that all components, chondrules, matrices, and CAIs, were affected by various degrees of secondary mineralization. Chondrules and CAIs are rimmed with fayalitic olivine [1, 2]; metal in all components is oxidized and sulfidized to magnetite, Ni-rich metal and sulfides [3]; silicates in all components are aqueously altered to phyllosilicates [4]; and nepheline, sodalite, wollastonite, and hedenbergite replace primary minerals in CAIs [5]. In those CV3s with altered CAIs, nepheline etc. are also present in chondrule mesostases [6] and in matrices [7]. Correlated occurrences of secondary minerals indicate that they have related origins. CV3 chondrites can be divided into three kinds according to their secondary features. Reduced CV3s (e.g., Efremovka) lack magnetite [8] and show minimal secondary features. Oxidized CV3s [8] generally show all features: those like Mokoia contain minor fayalitic rims, nepheline, etc, whereas those like Allende lack phyllosilicates but contain well developed fayalite rims and abundant nepheline, etc. Allende-like CV3 chondrites also contain abundant plate-like matrix olivine (Fa(sub)45-55). Similarities in chemistry and O isotopic composition and petrographic observations suggest that fayalitic rims and plate-like matrix olivine have related origins [1, 9]. The presence of secondary minerals in all components implies that alteration postdated component formation. The absence of secondary minerals in reduced CV3s indicates that CV3 oxidized formed from CV3 reduced-like material. Oxidized and reduced materials coexist in some breccias indicating a common parent asteroid. Nebular origins are widely accepted for most secondary features. To form fayalitic rims and matrix , Palme and colleagues [10, 11] suggest that chondritic components were briefly exposed to a hot (>1500 K), highly oxidizing nebula with H2O/H2 to about 1. Such an environment could have resulted from

  2. Modal Mineralogy of CV3 Chondrites by PSD-XRD: Mineralogic Insights into a Complex Evolutionary History

    NASA Astrophysics Data System (ADS)

    Howard, K. T.; Benedix, G. K.; Bland, P. A.; Cressey, G.

    2009-03-01

    CV3 chondrites Vigarano, Efremovka, Allende, Mokoia, Grosnaja and Kaba are amongst the most studied rocks in existence. By XRD we define the first quantitative modal mineralogy of these samples and explore implications of our data to petrogenesis.

  3. Thermal performance of a top heat mode closed-loop oscillating heat pipe with a check valve (THMCLOHP/CV)

    NASA Astrophysics Data System (ADS)

    Bhuwakietkumjohn, N.; Parametthanuwat, T.

    2015-05-01

    The aim of this research is to investigate the heat transfer characteristics of a top heat mode closed-looped oscillating heat pipe with check valves (THMCLOHP/CV). Ethanol is used as a working fluid with filling ratios of 30, 50, and 80% of the total volume of the tube. The THMCLOHP/CV is made of a copper tube with an inside diameter of 2.03 mm. The angle of inclination is 90° from the horizontal axis with 40 turns, two check valves, and an evaporator length of 50, 100, and 150 mm. The operating temperatures are 44 and 55°C. It is found that the thermal resistance decreases significantly as the working temperature is increased. Thus, the evaporator length affects the thermal resistance of the THMCLOHP/CV. The presence of the THMCLOHP/CV is clearly demonstrated to contribute to thermal performance improvement.

  4. Defective secretion of mucilage is the cellular basis for agravitropism in primary roots of Zea mays cv. Ageotropic

    NASA Technical Reports Server (NTRS)

    Miller, I.; Moore, R.

    1990-01-01

    Root caps of primary, secondary, and seminal roots of Z. mays cv. Kys secrete large amounts of mucilage and are in close contact with the root all along the root apex. These roots are strongly graviresponsive. Secondary and seminal roots of Z. mays cv. Ageotropic are also strongly graviresponsive. Similarly, their caps secrete mucilage and closely appress the root all along the root apex. However, primary roots of Z. mays cv. Ageotropic are non-responsive to gravity. Their caps secrete negligible amounts of mucilage and contact the root only at the extreme apex of the root along the calyptrogen. These roots become graviresponsive when their tips are coated with mucilage or mucilage-like materials. Peripheral cells of root caps of roots of Z. mays cv. Kys contain many dictyosomes associated with vesicles that migrate to and fuse with the plasmalemma. Root-cap cells of secondary and seminal (i.e. graviresponsive) roots of Z. mays cv. Ageotropic are similar to those of primary roots of Z. mays cv. Kys. However, root-cap cells of primary (i.e. non-graviresponsive) roots of Z. mays cv. Ageotropic have distended dictyosomal cisternae filled with an electron-dense, granular material. Large vesicles full of this material populate the cells and apparently do not fuse with the plasmalemma. Taken together, these results suggest that non-graviresponsiveness of primary roots of Z. mays cv. Ageotropic results from the lack of apoplastic continuity between the root and the periphery of the root cap. This is a result of negligible secretion of mucilage by cells along the edge of the root cap which, in turn, appears to be due to the malfunctioning of dictyosomes in these cells.

  5. Pulmonary nodule detection in CT images based on shape constraint CV model

    SciTech Connect

    Wang, Bing; Tian, Xuedong; Wang, Qian; Yang, Ying; Xie, Hongzhi E-mail: xiehongzhi@medmail.com.cn; Zhang, Shuyang; Gu, Lixu E-mail: xiehongzhi@medmail.com.cn

    2015-03-15

    Purpose: Accurate detection of pulmonary nodules remains a technical challenge in computer-aided diagnosis systems because some nodules may adhere to the blood vessels or the lung wall, which have low contrast compared to the surrounding tissues. In this paper, the analysis of typical shape features of candidate nodules based on a shape constraint Chan–Vese (CV) model combined with calculation of the number of blood branches adhered to nodule candidates is proposed to reduce false positive (FP) nodules from candidate nodules. Methods: The proposed scheme consists of three major stages: (1) Segmentation of lung parenchyma from computed tomography images. (2) Extraction of candidate nodules. (3) Reduction of FP nodules. A gray level enhancement combined with a spherical shape enhancement filter is introduced to extract the candidate nodules and their sphere-like contour regions. FPs are removed by analysis of the typical shape features of nodule candidates based on the CV model using spherical constraint and by investigating the number of blood branches adhered to the candidate nodules. The constrained shapes of CV model are automatically achieved from the extracted candidate nodules. Results: The detection performance was evaluated on 127 nodules of 103 cases including three types of challenging nodules, which are juxta-pleural nodules, juxta-vascular nodules, and ground glass opacity nodules. The free-receiver operating characteristic (FROC) curve shows that the proposed method is able to detect 88% of all the nodules in the data set with 4 FPs per case. Conclusions: Evaluation shows that the authors’ method is feasible and effective for detection of three types of nodules in this study.

  6. The first mass and angular momentum loss measurements for a CV-like binary

    NASA Astrophysics Data System (ADS)

    Drake, Jeremy

    2014-10-01

    The period distribution of close binaries, cataclysmic variables, novae and single-degenerate SN1a progenitor candidates is largely controlled by magnetically-driven mass and angular momentum loss (AML) from the M dwarf secondary. The mass loss rates for these spun-up stars remain essentially unknown and impossible to observe directly, with likely values in the range 1e-12 to 1e-15 Msun/yr. AML presciptions for CVs differ by orders of magnitude. One way to measure the mass loss rate is to observe the dM wind accrete onto its WD companion in a pre-CV very close to Roche Lobe overflow but lacking the obscuring complications and emission from an accretion disk. The measurement can be combined with realistic MHD models to understand the accretion fraction, the mass that escapes, and the AML. The best-studied nearby pre-CV is QS Vir (48pc, P=3.6hr). However, its wind accretion rates measured from 1999 HST UV spectra of the WD metal absorption lines and 2006 XMM-Newton CCD spectroscopy differ by a factor of a thousand, pointing to either a dominant CME stochastic component, or a "magnetic switch" found in MHD simulations and driven by cyclic activity on the M dwarf. HST COS spectra combined with XMM-Newton monitoring on timescales from weeks to years will tease out CME vs cyclic accretion variations. UV and X-ray measurements will provide the first consistency check of both accretion rate measurement methods. MHD models tailored to the system will enable the first quasi-direct measurements of the mass loss and AML from a CV-like binary. Our project requires 6 HST/COS orbits in Cycles 22-24, and 60ksec on XMM in Cycle 22

  7. Regeneration by somatic embryogenesis of triploid plants from endosperm of walnut, Juglans regia L. cv Manregian.

    PubMed

    Tulecke, W; McGranahan, G; Ahmadi, H

    1988-08-01

    Plants were regenerated by somatic embryogenesis from endosperm tissue of open-pollinated seeds of Juglans regia L. cv Manregian. These plants were obtained by growing endosperm tissue on media similar to those used for plant regeneration from walnut cotyledons (Tulecke and McGranahan 1985). The plants appear morphologically uniform and have a triploid chromosome number of 3n=48. Nine plants have been grown to a young sapling stage in soil. This embryogenic line from endosperm has been maintained in culture for two years by the process of repetitive somatic embryogenesis. PMID:24241869

  8. Dark inclusions in Allende, Leoville, and Vigarano - Evidence for nebular oxidation of CV3 constituents

    NASA Astrophysics Data System (ADS)

    Johnson, C. A.; Prinz, M.; Weisberg, M. K.; Clayton, R. N.; Mayeda, T. K.

    1990-03-01

    The origin and the history of dark inclusions (DIs) are investigated using petrologic, chemical, and oxygen isotopic data on ten DI samples from Allende, Leoville, and Vigarano. These data indicate that the DIs of the Leoville and Vigarano are closely similar to those of Allende. The inclusions appear to be fragments of CV3 parent bodies which were processed to different degrees prior to their incorporation as clasts into the Allende, Leoville, and Vigarano chondrites. The processing homogenized the olivine compositions, presumably through heating, and also involved oxygen exchange with O-16-poorer surroundings.

  9. Three in one go: consequential angular momentum loss can solve major problems of CV evolution

    NASA Astrophysics Data System (ADS)

    Schreiber, M. R.; Zorotovic, M.; Wijnen, T. P. G.

    2016-01-01

    The average white dwarf (WD) masses in cataclysmic variables (CVs) have been measured to significantly exceed those of single WDs, which is the opposite of what is theoretically expected. We present the results of binary population synthesis models taking into account consequential angular momentum loss (CAML) that is assumed to increase with decreasing WD mass. This approach cannot only solve the WD mass problem but also brings in agreement theoretical predictions and observations of the orbital period distribution and the space density of CVs. We speculate that frictional angular momentum loss following nova eruptions might cause such CAML and could be the missing ingredient of CV evolution.

  10. Organelle genome stability in anther-derived doubled haploids of wheat (Triticum aestivum L., cv. 'Moisson').

    PubMed

    Rode, A; Hartmann, C; Dron, M; Picard, E; Ouetier, F

    1985-12-01

    Chloroplast and mitochondrial compartments of a parental line of wheat (Triticum aestivum L., cv. 'Moisson') and its anther-derived doubled haploid lines have been analyzed and compared on the basis of their DNA restriction patterns. The results obtained show that no noticeable difference can be detected between doubled haploid lines and parental line at the level of ctDNA and mtDNA organization. It may be concluded that in vitro culture by itself does not systematically generate a cytoplasmic variation in germ cells. PMID:24247401

  11. Carrier Density Profiling of Ultra-Shallow Junction Layers Through Corrected C-V Plotting

    SciTech Connect

    Chen, James; Dimitrov, Dimitar; Dimitrova, Tatiana; Timans, Paul; Gelpey, Jeff; McCoy, Steve; Lerch, Wilfried; Paul, Silke; Bolze, Detlef

    2008-11-03

    The aim of this report is to present and justify a new approach for carrier density profiling in ultra-shallow junction (USJ) layer. This new approach is based on a capacitance measurement model, which takes series impedance, shunt resistance and the presence of a boron skin on the USJ layer into account. It allows us to extract the depletion layer capacitances in the USJ layer from C-V plotting more accurately and hence to obtain better carrier density profiles. Based on this new approach the carrier density profiles of different USJ layers with and without halo-style implants are obtained and discussed.

  12. Dark inclusions in Allende, Leoville, and Vigarano - Evidence for nebular oxidation of CV3 constituents

    NASA Technical Reports Server (NTRS)

    Johnson, Craig A.; Prinz, Martin; Weisberg, Michael K.; Clayton, Robert N.; Mayeda, Toshiko K.

    1990-01-01

    The origin and the history of dark inclusions (DIs) are investigated using petrologic, chemical, and oxygen isotopic data on ten DI samples from Allende, Leoville, and Vigarano. These data indicate that the DIs of the Leoville and Vigarano are closely similar to those of Allende. The inclusions appear to be fragments of CV3 parent bodies which were processed to different degrees prior to their incorporation as clasts into the Allende, Leoville, and Vigarano chondrites. The processing homogenized the olivine compositions, presumably through heating, and also involved oxygen exchange with O-16-poorer surroundings.

  13. Compositional and petrographic similarities of CV and CK chondrites: A single group with variations in textures and volatile concentrations attributable to impact heating, crushing and oxidation

    NASA Astrophysics Data System (ADS)

    Wasson, John T.; Isa, Junko; Rubin, Alan E.

    2013-05-01

    Greenwood et al. (2010) gathered data on O-isotopic and elemental compositions and reevaluated literature data for CV and CK chondrites. They concluded that these two chondrite groups originated on the same parent asteroid, with CK chondrites being metamorphosed CV chondrites (which are otherwise missing types 4 through 6). To test this interpretation we have gathered new instrumental neutron-activation-analysis (INAA) data for CV and CK chondrites and reexamined their petrographic features. The new INAA data like the older data show scatter attributable to weathering effects, but we conclude that the refractory lithophile abundances are the same in CV and CK, in agreement with the Greenwood et al. interpretation. Several volatile elements are significantly lower in CK than in CV chondrites. Among the elements we determine, the greatest difference between CV and CK is found for Br, for which the CV/CK ratio is ∼4; As and Sb are about 20% lower in CK than CV and smaller differences are observed for Zn, Ga and Se. It seems likely that volatiles were lost during impact-heating events that also provided the heat responsible for metamorphic recrystallization. Within statistical uncertainty, chondrules in CV and CK chondrites are the same size and have similar textural distributions. A significant petrographic difference between CK and CV chondrites cited by Kallemeyn et al. (1991) was the much higher percentage of igneous rims around CV chondrules. However, we now recognize that many chondrules in CK3.8 NWA 1559 have igneous rims and in CK4 chondrites, igneous rims are recognizable by their associated sulfide-rich rings; there are no quantifiable CV-CK differences in igneous-rim abundances. We used Ca and Al maps to show that CK chondrites have CAI abundances similar to those of CV chondrites. It thus appears that there are no resolvable pre-metamorphic petrographic differences between CV and CK chondrites. We recommend that the “CK” designation be abandoned and

  14. Antioxidant and anticancer aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena.

    PubMed

    Liu, Chi-Ming; Kao, Chiu-Li; Wu, Hui-Ming; Li, Wei-Jen; Huang, Cheng-Tsung; Li, Hsing-Tan; Chen, Chung-Yi

    2014-01-01

    Fifteen compounds were extracted and purified from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena. These compounds include liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) cepharadione B (11), β-sitostenone (12), stigmasta-4,22-dien-3-one (13) and two chlorophylls: pheophytin-a (14) and aristophyll-C (15). The anti-oxidation activity of the compounds was examined by antiradical scavenging, metal chelating and ferric reducing power assays. The results have shown that these compounds have antioxidative activity. The study has also examined the antiproliferation activity of the isolated compounds against human melanoma, prostate and gastric cancer cells. The results shown that 7-hydroxydehydronuciferine (10) significantly inhibited the proliferation of melanoma, prostate and gastric cancer cells. Together, these findings suggest that leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena are a good resource for obtaining the biologically active substances with antioxidant properties. PMID:25372397

  15. Biochemical markers assisted screening of Fusarium wilt resistant Musa paradisiaca (L.) cv. puttabale micropropagated clones.

    PubMed

    Venkatesh; Krishna, V; Kumar, K Girish; Pradeepa, K; Kumar, S R Santosh; Kumar, R Shashi

    2013-07-01

    An efficient protocol was standardized for screening of panama wilt resistant Musa paradisiaca cv. Puttabale clones, an endemic cultivar of Karnataka, India. The synergistic effect of 6-benzyleaminopurine (2 to 6 mg/L) and thidiazuron (0.1 to 0.5 mg/L) on MS medium provoked multiple shoot induction from the excised meristem. An average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/L 6-benzyleaminopurine and 0.3 mg/L thidiazuron concentrations. Elongation of shoots observed on 5 mg/L BAP augmented medium with a mean length of 8.38 +/- 0.30 shoots per propagule. For screening of disease resistant clones, multiple shoot buds were mutated with 0.4% ethyl-methane-sulfonate and cultured on MS medium supplemented with Fusarium oxysporum f. sp. cubense (FOC) culture filtrate (5-15%). Two month old co-cultivated secondary hardened plants were used for screening of disease resistance against FOC by the determination of biochemical markers such as total phenol, phenylalanine ammonia lyase, oxidative enzymes like peroxidase, polyphenol oxidase, catalase and PR-proteins like chitinase, beta-1-3 glucanase activities. The mutated clones of M. paradisiaca cv. Puttabale cultured on FOC culture filtrate showed significant increase in the levels of biochemical markers as an indicative of acquiring disease resistant characteristics to FOC wilt. PMID:23898552

  16. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds.

    PubMed

    Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative identification of the compounds present in the lowest and highest yield extracts. Analysis of the leaf extract with phytochemical tests showed the presence of alkaloids, flavonoids and terpenes. Antifungal effectiveness was determined by challenging the extracts (LE, SRE, SUE) from the best extraction treatment against three phytopathogenic fungi: Rhizopus stolonifer, Fusarium spp. and Colletotrichum gloeosporioides. The leaf extract exhibited the broadest action spectrum. The MIC(50) for the leaf extract was 0.625 mg ml(-1) for Fusarium spp. and >10 mg ml(-1) for C. gloeosporioides, both equal to approximately 20% mycelial growth inhibition. Ethanolic extracts from Carica papaya L. cv. Maradol leaves are a potential source of secondary metabolites with antifungal properties. PMID:22282629

  17. Determination of polyphenols and antioxidant activity of Vitis labrusca cv. baile berries.

    PubMed

    Nile, Shivraj Hariram; Park, Se Won

    2015-10-01

    Grape juice and grape skin extracts are important commercial source of polyphenolic compounds which exert different functional properties such as color potential, antimicrobial, antioxidant activity, and health benefits. In this paper we describe a sensitive and specific assay for determination of bioactive polyphenolic compounds in Campbell Early (Vitis labrusca cv. baile). Five polyphenolic components were separated on an Agilent Zorbax Extend C18 Column (250 mm x 4.6 mm x 5 μm) and detected by a diode array detector. The mobile phase was composed of (a) aqueous phosphoric acid (0.2%, v/v); and (b) acetonitrile using a gradient elution. Analytes were performed at 25 degrees C with a flow rate of 0.8 ml/min and UV detection at 280, 360, and 520 nm. All calibration curves showed good linear regression (r2 ≥ 0.9999) within tested ranges. Overall intra- and inter-day variations were less than 1.90%, and the average recoveries were 95.5-105% for analytes. The antioxidant activity determined by DPPH radical assay, ranged from 86-105 for extracts, and 165-252 for studied standards (μM trolox/100 g dry wt.). The proposed method would be sensitive enough and reliable for quality control in functional food and modernization of Campbell Early (Vitis labrusca cv. baile) as potent antioxidant agents. PMID:26665298

  18. Biophenols from Table Olive cv Bella di Cerignola: Chemical Characterization, Bioaccessibility, and Intestinal Absorption.

    PubMed

    D'Antuono, Isabella; Garbetta, Antonella; Ciasca, Biancamaria; Linsalata, Vito; Minervini, Fiorenza; Lattanzio, Veronica M T; Logrieco, Antonio F; Cardinali, Angela

    2016-07-20

    In this study, the naturally debittered table olives cv Bella di Cerignola were studied in order to (i) characterize their phenolic composition; (ii) evaluate the polyphenols bioaccessibility; (iii) assess their absorption and transport, across Caco2/TC7. LC-MS/MS analysis has confirmed the presence of hydroxytyrosol acetate, caffeoyl-6'-secologanoside, and comselogoside. In vitro bioaccessibility ranged from 7% of luteolin to 100% of tyrosol, highlighting the flavonoids sensitivity to the digestive conditions. The Caco2/TC7 polyphenols accumulation was rapid (60 min) with an efficiency of 0.89%; the overall bioavailability was 1.86% (120 min), with hydroxytyrosol and tyrosol the highest bioavailables, followed by verbascoside and luteolin. In the cells and basolateral side, caffeic and coumaric acids metabolites, probably derived from esterase activities, were detected. In conclusion, the naturally debittered table olives cv Bella di Cerignola can be considered as a source of bioaccessible, absorbable, and bioavailable polyphenols that, for their potential health promoting effect, permit inclusion of table olives as a functional food suitable for a balanced diet. PMID:27355793

  19. In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua.

    PubMed

    Gu, X F; Yang, A F; Meng, H; Zhang, J R

    2005-12-01

    Tetraploid plants of Zizyphus jujuba Mill. cv. Zhanhua were obtained with in vitro colchicine treatment. Shoot tips from in vitro-grown plants were treated with five different concentrations of colchicine (0.01, 0.03, 0.05, 0.1, 0.3%) in liquid MS medium (Murashige and Skoog 1962), and shaken (100 rpm) at 25 degrees C in darkness for 24, 48, 72 or 96 h, respectively. Tetraploids were obtained at a frequency of over 3% by using 0.05% colchicine (48 h, 72 h) and 0.1% colchicine (24 h, 48 h) treatment as determined by flow cytometry. Cytological and morphological evidence confirmed the results of flow cytometric analysis. The chromosome number of diploid plants was 24 and that of tetraploid plants was 48. The stomata sizes of tetraploid plants were significantly larger than those of diploid plants, while the frequency of stomata were reduced significantly. Similarly, the chloroplast number of guard cells of tetraploid plants increased significantly. The selected tetraploid plants were grafted onto mature trees of Z. jujuba Mill. cv. Zhanhua in the field, resulted in thicker stems, rounder and succulent leaves, larger flowers and a delay in florescence time (3-4 days later) than diploid plants. PMID:16094528

  20. Multi-camera calibration based on openCV and multi-view registration

    NASA Astrophysics Data System (ADS)

    Deng, Xiao-ming; Wan, Xiong; Zhang, Zhi-min; Leng, Bi-yan; Lou, Ning-ning; He, Shuai

    2010-10-01

    For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the corresponding relationship between each camera view, the computation of the rotation matrix and translation matrix is formulated as a constrained optimization problem. According to the Kuhn-Tucker theorem and the properties on the derivative of the matrix-valued function, the formulae of rotation matrix and translation matrix are deduced by using singular value decomposition algorithm. Afterwards an iterative method is utilized to get the entire coordinate transformation of pair-wise views, thus the precise multi-view registration can be conveniently achieved and then can get the relative positions in them(the camera outside the parameters).Experimental results show that the method is practical in multi-camera calibration .

  1. Heterogeneous compute in computer vision: OpenCL in OpenCV

    NASA Astrophysics Data System (ADS)

    Gasparakis, Harris

    2014-02-01

    We explore the relevance of Heterogeneous System Architecture (HSA) in Computer Vision, both as a long term vision, and as a near term emerging reality via the recently ratified OpenCL 2.0 Khronos standard. After a brief review of OpenCL 1.2 and 2.0, including HSA features such as Shared Virtual Memory (SVM) and platform atomics, we identify what genres of Computer Vision workloads stand to benefit by leveraging those features, and we suggest a new mental framework that replaces GPU compute with hybrid HSA APU compute. As a case in point, we discuss, in some detail, popular object recognition algorithms (part-based models), emphasizing the interplay and concurrent collaboration between the GPU and CPU. We conclude by describing how OpenCL has been incorporated in OpenCV, a popular open source computer vision library, emphasizing recent work on the Transparent API, to appear in OpenCV 3.0, which unifies the native CPU and OpenCL execution paths under a single API, allowing the same code to execute either on CPU or on a OpenCL enabled device, without even recompiling.

  2. The Glycine max cv. Enrei Genome for Improvement of Japanese Soybean Cultivars

    PubMed Central

    Shimomura, Michihiko; Kanamori, Hiroyuki; Komatsu, Setsuko; Namiki, Nobukazu; Mukai, Yoshiyuki; Kurita, Kanako; Kamatsuki, Kaori; Ikawa, Hiroshi; Yano, Ryoichi; Ishimoto, Masao; Kaga, Akito; Katayose, Yuichi

    2015-01-01

    We elucidated the genome sequence of Glycine max cv. Enrei to provide a reference for characterization of Japanese domestic soybean cultivars. The whole genome sequence obtained using a next-generation sequencer was used for reference mapping into the current genome assembly of G. max cv. Williams 82 obtained by the Soybean Genome Sequencing Consortium in the USA. After sequencing and assembling the whole genome shotgun reads, we obtained a data set with about 928 Mbs total bases and 60,838 gene models. Phylogenetic analysis provided glimpses into the ancestral relationships of both cultivars and their divergence from the complex that include the wild relatives of soybean. The gene models were analyzed in relation to traits associated with anthocyanin and flavonoid biosynthesis and an overall profile of the proteome. The sequence data are made available in DAIZUbase in order to provide a comprehensive informatics resource for comparative genomics of a wide range of soybean cultivars in Japan and a reference tool for improvement of soybean cultivars worldwide. PMID:26199933

  3. CV Protection in the EMPA-REG OUTCOME Trial: A "Thrifty Substrate" Hypothesis.

    PubMed

    Ferrannini, Ele; Mark, Michael; Mayoux, Eric

    2016-07-01

    The striking and unexpected relative risk reductions in cardiovascular (CV) mortality (38%), hospitalization for heart failure (35%), and death from any cause (32%) observed in the EMPA-REG OUTCOME trial using an inhibitor of sodium-glucose cotransporter 2 (SGLT2) in patients with type 2 diabetes and high CV risk have raised the possibility that mechanisms other than those observed in the trial-modest improvement in glycemic control, small decrease in body weight, and persistent reductions in blood pressure and uric acid level-may be at play. We hypothesize that under conditions of mild, persistent hyperketonemia, such as those that prevail during treatment with SGLT2 inhibitors, β-hydroxybutyrate is freely taken up by the heart (among other organs) and oxidized in preference to fatty acids. This fuel selection improves the transduction of oxygen consumption into work efficiency at the mitochondrial level. In addition, the hemoconcentration that typically follows SGLT2 inhibition enhances oxygen release to the tissues, thereby establishing a powerful synergy with the metabolic substrate shift. These mechanisms would cooperate with other SGLT2 inhibition-induced changes (chiefly, enhanced diuresis and reduced blood pressure) to achieve the degree of cardioprotection revealed in the EMPA-REG OUTCOME trial. This hypothesis opens up new lines of investigation into the pathogenesis and treatment of diabetic and nondiabetic heart disease. PMID:27289126

  4. Gene ontology based characterization of expressed sequence tags (ESTs) of Brassica rapa cv. Osome.

    PubMed

    Arasan, Senthil Kumar Thamil; Park, Jong-In; Ahmed, Nasar Uddin; Jung, Hee-Jeong; Lee, In-Ho; Cho, Yong-Gu; Lim, Yong-Pyo; Kang, Kwon-Kyoo; Nou, Ill-Sup

    2013-07-01

    Chinese cabbage (Brassica rapa) is widely recognized for its economic importance and contribution to human nutrition but abiotic and biotic stresses are main obstacle for its quality, nutritional status and production. In this study, 3,429 Express Sequence Tag (EST) sequences were generated from B. rapa cv. Osome cDNA library and the unique transcripts were classified functionally using a gene ontology (GO) hierarchy, Kyoto encyclopedia of genes and genomes (KEGG). KEGG orthology and the structural domain data were obtained from the biological database for stress related genes (SRG). EST datasets provided a wide outlook of functional characterization of B. rapa cv. Osome. In silico analysis revealed % 83 of ESTs to be well annotated towards reeds one dimensional concept. Clustering of ESTs returned 333 contigs and 2,446 singlets, giving a total of 3,284 putative unigene sequences. This dataset contained 1,017 EST sequences functionally annotated to stress responses and from which expression of randomly selected SRGs were analyzed against cold, salt, drought, ABA, water and PEG stresses. Most of the SRGs showed differentially expression against these stresses. Thus, the EST dataset is very important for discovering the potential genes related to stress resistance in Chinese cabbage, and can be of useful resources for genetic engineering of Brassica sp. PMID:23898551

  5. Galectin CvGal2 from the Eastern Oyster (Crassostrea virginica) Displays Unique Specificity for ABH Blood Group Oligosaccharides and Differentially Recognizes Sympatric Perkinsus Species.

    PubMed

    Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N; Bachvaroff, Tsvetan R; Tasumi, Satoshi; Pasek, Marta; Banerjee, Aditi; Shridhar, Surekha; Wang, Lai-Xi; Bianchet, Mario A; Vasta, Gerardo R

    2015-08-01

    Galectins are highly conserved lectins that are key to multiple biological functions, including pathogen recognition and regulation of immune responses. We previously reported that CvGal1, a galectin expressed in phagocytic cells (hemocytes) of the eastern oyster (Crassostrea virginica), is hijacked by the parasite Perkinsus marinus to enter the host, where it causes systemic infection and death. Screening of an oyster hemocyte cDNA library revealed a novel galectin, which we designated CvGal2, with four tandemly arrayed carbohydrate recognition domains (CRDs). Phylogentic analysis of the CvGal2 CRDs suggests close relationships with homologous CRDs from CvGal1. Glycan array analysis, however, revealed that, unlike CvGal1 which preferentially binds to the blood group A tetrasaccharide, CvGal2 recognizes both blood group A and B tetrasaccharides and related structures, suggesting that CvGal2 has broader binding specificity. Furthermore, SPR analysis demonstrated significant differences in the binding kinetics of CvGal1 and CvGal2, and structural modeling revealed substantial differences in their interactions with the oligosaccharide ligands. CvGal2 is homogeneously distributed in the hemocyte cytoplasm, is released to the extracellular space, and binds to the hemocyte surface. CvGal2 binds to P. marinus trophozoites in a dose-dependent and β-galactoside-specific manner. Strikingly, negligible binding of CvGal2 was observed for Perkinsus chesapeaki, a sympatric parasite species mostly prevalent in the clams Mya arenaria and Macoma balthica. The differential recognition of Perkinsus species by the oyster galectins is consistent with their relative prevalence in oyster and clam species and supports their role in facilitating parasite entry and infectivity in a host-preferential manner. PMID:26158802

  6. Determination of the petrologic type of CV3 chondrites by Raman spectroscopy of included organic matter

    NASA Astrophysics Data System (ADS)

    Bonal, Lydie; Quirico, Eric; Bourot-Denise, Michèle; Montagnac, Gilles

    2006-04-01

    This paper reports the first reliable quantitative determination of the thermal metamorphism grade of a series of nine CV3 chondrites: Allende, Axtell, Bali, Mokoia, Grosnaja, Efremovka, Vigarano, Leoville, and Kaba. The maturity of the organic matter in matrix, determined by Raman spectroscopy, has been used as a powerful metamorphic tracer, independent of the mineralogical context and extent of aqueous alteration. This tracer has been used along with other metamorphic tracers such as Fe zoning in type-I chondrules of olivine phenocrysts, presolar grain abundance and noble gas abundance (bulk and P3 component). The study shows that the petrologic types determined earlier by Induced ThermoLuminescence were underestimated and suggests the following values: PT (Allende-Axtell) >3.6; PT (Bali-Mokoia-Grosnaja) ˜3.6; PT (Efremovka-Leoville-Vigarano) = 3.1-3.4; PT (Kaba) ˜3.1. The most commonly studied CV3, Allende, is also the most metamorphosed. Bali is a breccia containing clasts of different petrologic types. The attribution suggested by this study is that of clasts of the highest petrologic types, as pointed out by IOM maturity and noble gas bulk abundance. CV3 chondrites have complex asteroidal backgrounds, with various degrees of aqueous alteration and/or thermal metamorphism leading to complex mineralogical and petrologic patterns. (Fe,Mg) chemical zoning in olivine phenocrysts, on the borders of type I chondrules of porphyritic olivine- and pyroxene-rich textural types, has been found to correlate with the metamorphism grade. This suggests that chemical zoning in some chondrules, often interpreted as exchanges between chondrules and nebular gas, may well have an asteroidal origin. Furthermore, the compositional range of olivine matrix is controlled both by thermal metamorphism and aqueous alteration. This does not support evidence of a nebular origin and does not necessarily mirror the metamorphism grade through (Fe,Mg) equilibration. On the other hand, it may

  7. Electroacupuncture stimulation at CV4 prevents ovariectomy-induced osteoporosis in rats via Wnt-β-catenin signaling

    PubMed Central

    FAN, HUAILING; JI, FENG; LIN, YING; ZHANG, MULAN; QIN, WEI; ZHOU, QI; WU, QIANG

    2016-01-01

    The present study aimed to investigate the effect of electroacupuncture stimulation at CV4 (also termed Guanyuan) on femoral osteocalcin also termed bone gla protein (BGP), alkaline phosphatase (ALP), bone mineral density (BMD) and biomechanics, as well as the Wnt-β-catenin signaling pathway in rats with postmenopausal osteoporosis. Female Sprague-Dawley rats (4.5-months old) were randomly divided into sham, Ovx, CV4 and mock groups (n=10/group). With the exception of those in the sham group, the rats were ovariectomized to induce postmenopausal osteoporosis. The rats in the CV4 and mock groups were given electroacupuncture at CV4 and non-acupoint, respectively. The rats in the Ovx model and sham groups underwent identical fixing procedures, but did not undergo electroacupuncture. Following treatment, hematoxylin and eosin staining was used to observe morphological changes in the left femoral trabecular bone, and a three-point-bending test was used to analyze femur biomechanics and determine the BMD. In addition, an enzyme-linked immunosorbent assay was used to measure the serum levels of ALP/BGP and reverse transcription-quantitative polymerase chain reaction was used detect the expression levels of Wnt3a, β-catenin and Runx2. In the present study, it was demonstrated that electroacupuncture at CV4 significantly improved the osteoporotic morphological changes that occurred in the ovariectomized rats, increased serum ALP and BGP levels, enhanced the maximum and fracture loads, increased BMD (P<0.01), and activated the Wnt-β-catenin signaling pathway. These findings demonstrated that electroacupuncture stimulation at CV4 affected bone formation and promoted bone metabolism in rats with postmenopausal osteoporosis, possibly by activating the Wnt-β-catenin signaling pathway. PMID:26846191

  8. More evidence for a partially differentiated CV chondrite parent body from paleomagnetic studies of ALH 84028 and ALH 85006

    NASA Astrophysics Data System (ADS)

    Klein, B. Z.; Weiss, B. P.; Carporzen, L.

    2014-12-01

    Recent paleomagnetic studies of the CV carbonaceous chondrites Allende and Kaba and numerical modeling studies have suggested that the CV chondrite parent body may have been partially differentiated, with a molten metallic core, dynamo magnetic field, and an unmelted chondritic lid. To further evaluate this hypothesis, here we present new paleomagnetic analyses of two previously unstudied CV3 chondrites: the unshocked, Allende-type oxidized chondrite ALH 84028 and the weakly shocked, Bali-type oxidized chondrite ALH 85006. We preformed alternating field (AF) and thermal demagnetization experiments, AF-based paleointensity experiments, and rock magnetic experiments on mutually oriented subsamples of each meteorite. Both meteorites pass fusion crust baked contact tests, indicating that their interiors retain a magnetization predating atmospheric entry. In the interior of ALH 84028, we identified a unidirectional medium temperature (blocked to 300°C), high coercivity (blocked to >420 mT) component. In the interior of ALH 85006, we identified MT components blocked up to 400-475°C. The unblocking temperatures and unidirectional nature of the MT components in both meteorites indicates their origin as a partial thermoremanence or thermochemical remanence acquired during metamorphism following accretion of the CV chondrite parent body. Our paleointensity experiments indicate paleofield intensities of 32-73 μT for ALH 84028 and 14-45 μT for ALH 85006 . When combined with similar recent results for Allende and Kaba, there is now consistent evidence for dynamo fields from four CV chondrites with collectively diverse lithologies and shock states. Therefore, the magnetic field on the CV parent body was not a localized event like that expected for a field generated by meteoroid impact plasmas and instead likely had a wide spatial extent. Further, given the younger I-Xe ages for Kaba compared to Allende (9-10 Ma and 2-3 Ma after Stillwater respectively), CV parent body

  9. Chemical and physical studies of type 3 chondrites 12: The metamorphic history of CV chondrites and their components

    NASA Technical Reports Server (NTRS)

    Guimon, R. Kyle; Symes, Steven J. K.; Sears, Derek W. G.

    1995-01-01

    The induced thermoluminescence (TL) properties of 16 CV and CV-related chondrites, four CK chondrites and Renazzo (CR2) have been measured in order to investigate their metamorphic history. The petrographic, mineralogical and bulk compositional differences among the CV chondrites indicate that the TL sensitivity of the approximately 130 C TL peak is reflecting the abundance of ordered feldspar, especially in chondrule mesostasis, which in turn reflects parent-body metamorphism. The TL properties of 18 samples of homogenized Allende powder heated at a variety of times and temperatures, and cathodoluminescence mosaics of Axtell and Coolidge, showed results consistent with this conclusion. Five refractory inclusions from Allende, and separates from those inclusions, were also examined and yielded trends reflecting variations in mineralogy indicative of high peak temperatures (either metamorphic or igneous) and fairly rapid cooling. The CK chondrites are unique among metamorphosed chondrites in showing no detectable induced TL, which is consistent with literature data that suggests very unusual feldspar in these meteorites. Using TL sensitivity and several mineral systems and allowing for the differences in the oxidized and reduced subgroups, the CV and CV-related meteorites can be divided into petrologic types analogous to those of the ordinary and CO type 3 chondrites. Axtell, Kaba, Leoville, Bali, Arch and ALHA81003 are type 3.0-3.1, while ALH84018, Efremovka, Grosnaja, Allende and Vigarano are type 3.2-3.3 and Coolidge and Loongana 001 are type 3.8. Mokoia is probably a breccia with regions ranging in petrologic type from 3.0 to 3.2. Renazzo often plots at the end of the reduced and oxidized CV chondrite trends, even when those trends diverge, suggesting that in many respects it resembles the unmetamorphosed precursors of the CV chondrites. The low-petrographic types and low-TL peak temperatures of all samples, including the CV3.8 chondrites, indicates metamorphism

  10. Sonication inhibited browning but decreased polyphenols contents and antioxidant activity of fresh apple (malus pumila mill, cv. Red Fuji) juice.

    PubMed

    Sun, Yujing; Zhong, Liezhou; Cao, Lianfei; Lin, Wenwen; Ye, Xingqian

    2015-12-01

    Enzyme browning is the main challenge in the preparation of fresh apple juice. The influence of sonication on browning, as well as polyphenols and antioxidant activity of fresh apple juice was investigated. It was found that ultrasound can inhibit the browning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice, but decreased the contents of total phenolic content (TPC), total flavonoid content (TFC) and chlorogenic acid and reduced the antioxidant activity. On the whole, ultrasound technology cannot be used to the antibrowning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice. PMID:26604412

  11. C-V measurements of micron diameter metal-oxide-semiconductor capacitors using a scanning-electron-microscope-based nanoprobe.

    PubMed

    Zheng, T; Jia, H; Wallace, R M; Gnade, B E

    2007-10-01

    The C-V electrical characterization of microstructures on a standard probe station is limited by the magnification of the imaging system and the precision of the probe manipulators. To overcome these limitations, we examine the combination of in situ electrical probing and a dual column scanning electron microscope/focused ion beam system. The imaging parameters and probing procedures are carefully chosen to reduce e-beam damage to the metal oxide semiconductor capacitor device under test. Estimation of shunt capacitance is critical when making femtofarad level measurements. C-V measurements of micron size metal-oxide-silicon capacitors are demonstrated. PMID:17979444

  12. Humudifucol and Bioactive Prenylated Polyphenols from Hops (Humulus lupulus cv. "Cascade").

    PubMed

    Forino, Martino; Pace, Simona; Chianese, Giuseppina; Santagostini, Laura; Werner, Markus; Weinigel, Christina; Rummler, Silke; Fico, Gelsomina; Werz, Oliver; Taglialatela-Scafati, Orazio

    2016-03-25

    Humulus lupulus (hop plant) has long been used in traditional medicine as a sedative and antimicrobial agent. More recently, attention has been devoted to the phytoestrogenic activity of the plant extracts as well as to the anti-inflammatory and chemopreventive properties of the prenylated chalcones present. In this study, an Italian sample of H. lupulus cv. "Cascade" has been investigated and three new compounds [4-hydroxycolupulone (6), humudifucol (7) and cascadone (8)] have been purified and identified by means of NMR spectroscopy along with four known metabolites. Notably, humudifucol (7) is the first prenylated dimeric phlorotannin discovered in nature. Because structurally related phloroglucinols from natural sources were found previously to inhibit microsomal prostaglandin E2 synthase (mPGES)-1 and 5-lipoxygenase (5-LO), the isolated compounds were evaluated for their bioactivity against these pro-inflammatory target proteins. The prenylated chalcone xanthohumol inhibited both enzymes at low μM concentrations. PMID:26918635

  13. Influence of different drying methods on carotenoids and capsaicinoids of paprika (Cv., Jalapeno).

    PubMed

    Topuz, Ayhan; Dincer, Cuneyt; Ozdemir, Kubra Sultan; Feng, Hao; Kushad, Mosbah

    2011-12-01

    Influence of Refractance Window™ Drying (RWD), a novel contact drying method, on carotenoids, capsaicinoids, Retinol Activity Equivalent (RAE) and Scoville Heat Unit (SHU) of paprika (Cv., Jalapeno) was investigated in comparison with freeze drying (FD), oven drying (OD), and natural convective drying (NCD) methods. Eight carotenoids (capsanthin, capsorubin, capsolutein, β-carotene, β-cryptoxanthin, mutatoxanthin, violaxanthin and zeaxanthin) and five capsaicinoid analogues (capsaicin, dihydrocapsaicin, homodihydrocapsaicin, isodihydrocapsaicin, nordihydrocapsaicin) were identified in paprika. All these components were significantly (P<0.05) decreased by the RWD, FD and OD methods. However, due to ongoing synthesis, the NCD method resulted in higher carotenoids, except violaxanthin and mutatoxanthin, and capsaicinoids content than those of the others, even puree. Mutatoxanthin, naturally occurring pigment in red pepper, could only be detected in FD paprika. The highest RAE and SHU values, which were derived from the data of carotenoids and capsaicinoids, respectively, were also determined in NCD paprika. PMID:25212310

  14. Overseas trip report, CV 990 underflight mission. [Norwegian Sea, Greenland ice sheet, and Alaska

    NASA Technical Reports Server (NTRS)

    Gloersen, P.; Crawford, J.; Hardis, L.

    1980-01-01

    The scanning microwave radiometer-7 simulator, the ocean temperature scanner, and an imaging scatterometer/altimeter operating at 14 GHz were carried onboard the NASA CV-990 over open oceans, sea ice, and continental ice sheets to gather surface truth information. Data flights were conducted over the Norwegian Sea to map the ocean polar front south and west of Bear Island and to transect several Nimbus-7 footprints in a rectangular pattern parallel to the northern shoreline of Norway. Additional flights were conducted to obtain correlative data on the cryosphere parameters and characteristics of the Greenland ice sheet, and study the frozen lakes near Barrow. The weather conditions and flight path way points for each of the nineteen flights are presented in tables and maps.

  15. Partial melting of the Allende (CV3) meteorite - Implications for origins of basaltic meteorites

    NASA Technical Reports Server (NTRS)

    Jurewicz, A. J. G.; Mittlefehldt, D. W.; Jones, J. H.

    1991-01-01

    Eucrites and angrites are distinct types of basaltic meteorites whose origins are poorly known. Experiments in which samples of the Allende (CV3) carbonaceous chondrite were partially melted indicate that partial melts can resemble either eucrites or angrites, depending only on the oxygen fugacity. Melts are eucritic if this variable is below that of the iron-wuestite buffer or angritic if above it. With changing pressure, the graphite-oxygen redox reaction can produce oxygen fugacities that are above or below those of the iron-wuestite buffer. Therefore, a single, homogeneous, carbonaceous planetoid greater than 110 kilometers in radius could produce melts of drastically different composition, depending on the depth of melting.

  16. Metal phase in a B1-type CAI fragment of the CV Efremovka chondrite

    NASA Astrophysics Data System (ADS)

    Fisenko, A. V.; Ignatenko, K. I.; Lavrukhina, A. K.

    1991-09-01

    Results are presented from petrographical, mineralogical, and chemical analyses of metal particles in two B1-type CAI fragments obtained from the Efremovka CV chondrite. The fragments were found to have a broken outer border consisting mainly of grains of Ca phosphates and a Fe/Ni phase. Both fragments are associated with V2O3-rich pyroxene. All individual particles and veins of the fragments are made up from high-Ni tenite, sometimes enriched in V. It is suggested that all features of the metal phase of this chondrite are a consequence of oxidation, or of partial evaporation of the metal followed by its oxidation, and that the characteristics of the metal phase of the CAI phase of the Efremovka chondrite may correspond to those of the protomatter of some fremdlings, such as the Allende chondrite.

  17. Petrography and classification of refractory inclusions in the Allende and Mokoia CV3 chondrites

    NASA Technical Reports Server (NTRS)

    Kornacki, A. S.; Cohen, R. E.; Wood, J. A.

    1983-01-01

    Results are reported for a comprehensive petrographic survey of the refractory inclusions in the Allende and Mokoia CV3 chondrites. More than 600 refractory inclusions in 22 thin sections of the meteorites were studied by optical and scanning-electron microscopy. Olivine-rich inclusions and Ca, Al-rich inclusions (CAIs) are aggregates of various combinations of three fundamental petrographic constituents: rimmed concentric objects, Ca, Si-rich chaotic material, and mafic inclusion matrix. A new classification system for refractory inclusions is developed that is based on the size and abundance of these three fundamental constituents. The new classification system avoids several problems that are inherent in other classification systems, which use the term 'coarse-grained' too restrictively for many simple CAIs and inaccurately for most mililite-rich complex CAIs.

  18. The fractionation of noble gases in diamonds of CV3 Efremovka chondrite

    NASA Technical Reports Server (NTRS)

    Fisenko, A. V.; Verchovsky, A. B.; Semjonova, L. F.; Shukolyukov, Yu. A.

    1993-01-01

    It was shown that in diamonds of Efremovka CV3 the noble gases with normal isotopic compositions are fractionated in different degree while the correlation of isotopic anomalous components is nearly constant. Some data for noble gases in DE-4 sample of Efremovka chondrite are considered. In contrast to DE-2 sample the DE-4 was treated except conc. HClO4, 220 C in addition with mixture of conc. H2SO4+H3PO4 (1:1), 220 C, twice. Noble gases analysis were performed in Germany at Max Plank Institute fur Chemie. Noble gases were released by oxidation of samples at stepped heating from 420 C to 810 C and by pyrolysis at 580, 590, and 680 C.

  19. Inducing gravitropic curvature of primary roots of Zea mays cv Ageotropic

    NASA Technical Reports Server (NTRS)

    Moore, R.; Evans, M. L.; Fondren, W. M.

    1990-01-01

    Primary roots of the mutant 'Ageotropic' cultivar of Zea mays are nonresponsive to gravity. Their root caps secrete little or no mucilage and touch the root only at the extreme apex. A gap separates the cap and root at the periphery of the cap. Applying mucilage from normal roots or substances with a consistency similar to that of mucilage to tips of mutant roots causes these roots to become strongly graviresponsive. Gravicurvature stops when these substances are removed. Caps of some mutants secrete small amounts of mucilage and are graviresponsive. These results indicate that (a) the lack of graviresponsiveness in the mutant results from disrupting the transport pathway between the cap and root, (b) movement of the growth-modifying signal from the cap to the root occurs via an apoplastic pathway, and (c) mucilage is necessary for normal communication between the root cap and root in Zea mays cv Ageotropic.

  20. Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering.

    PubMed

    Sahni, Sumit; Ganie, Showkat Hussain; Narula, Alka; Srivastava, Prem Shankar; Singh, Hari Bansh

    2013-07-01

    High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B. juncea cv. Geeta. Hypocotyl from 5-day-old seedlings were used as explants. Of the various combinations of auxins and cytokinins tried along with Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium, MS + IAA (0.2 mg/l) + BA (3 mg/l) proved best for shoot regeneration with 89.9 % regeneration efficiency. To induce early flowering Leafy gene from Arabidopsis thaliana was transformed using Agrobacterium mediated transformation method. After 12 weeks transgenic plants showed flowering in vitro whereas their untransformed counterpart did not flower even after 16 weeks. The maximum transformation frequency was 4 %. PMID:24431514

  1. Analysis of ultraviolet atmospheric eclipses in the Wolf-Rayet binary CV Serpentis

    NASA Technical Reports Server (NTRS)

    Eaton, J. A.; Cherepashchuk, A. M.; Khaliullin, Kh. F.

    1985-01-01

    While no eclipses deeper than 0.04 mag are noted in the present UV spectra, covering one-half of an orbital cycle of CV Ser, in the electron scattering continuum at 2400-3200 A or in fine error sensor observations, marked atmospheric eclipses of up to 0.5 mag depth are observed in individual strong lines and over large ranges of the continuum at shorter wavelengths. The flux above the continuum in the C II 1247 A, Si IV 1400 A, and Si IV 1723 lines showed similar phase dependence with emission weakening, as well as with the emission's going into absorption as phase progresses from superior to inferior conjunction of the WC star (primary eclipse). These observations show effects very similar to the behavior of WN stars in the UV.

  2. Flight-determined derivatives and dynamic characteristics of the CV-990 airplane

    NASA Technical Reports Server (NTRS)

    Gilyard, G. B.

    1972-01-01

    Flight-determined longitudinal and lateral-directional stability and control derivatives are presented for the CV-990 airplane for various combinations of Mach number, altitude, and flap setting throughout the flight envelope up to a Mach number of 0.87. Also presented are the dynamic characteristics of the aircraft calculated from the flight-obtained derivatives and the measured phugoid characteristics. The derivative characteristics were obtained from flight records of longitudinal and lateral-directional transient oscillation maneuvers by using a modified Newton-Raphson digital derivative determination technique. Generally the derivatives exhibited consistent variation with lift coefficient in the low-speed data and with Mach number and altitude in the high-speed data. Many also varied with flap deflection, notably spoiler effectiveness and directional stability.

  3. Defect Detection in Superconducting Radiofrequency Cavity Surface Using C + + and OpenCV

    NASA Astrophysics Data System (ADS)

    Oswald, Samantha; Thomas Jefferson National Accelerator Facility Collaboration

    2014-03-01

    Thomas Jefferson National Accelerator Facility (TJNAF) uses superconducting radiofrequency (SRF) cavities to accelerate an electron beam. If theses cavities have a small particle or defect, it can degrade the performance of the cavity. The problem at hand is inspecting the cavity for defects, little bubbles of niobium on the surface of the cavity. Thousands of pictures have to be taken of a single cavity and then looked through to see how many defects were found. A C + + program with Open Source Computer Vision (OpenCV) was constructed to reduce the number of hours searching through the images and finds all the defects. Using this code, the SRF group is now able to use the code to identify defects in on-going tests of SRF cavities. Real time detection is the next step so that instead of taking pictures when looking at the cavity, the camera will detect all the defects.

  4. Contrasting Size Distributions of Chondrules and Inclusions in Allende CV3

    NASA Technical Reports Server (NTRS)

    Fisher, Kent R.; Tait, Alastair W.; Simon, Jusin I.; Cuzzi, Jeff N.

    2014-01-01

    There are several leading theories on the processes that led to the formation of chondrites, e.g., sorting by mass, by X-winds, turbulent concentration, and by photophoresis. The juxtaposition of refractory inclusions (CAIs) and less refractory chondrules is central to these theories and there is much to be learned from their relative size distributions. There have been a number of studies into size distributions of particles in chondrites but only on relatively small scales primarily for chondrules, and rarely for both Calcium Aluminum-rich Inclusions (CAIs) and chondrules in the same sample. We have implemented macro-scale (25 cm diameter sample) and high-resolution microscale sampling of the Allende CV3 chondrite to create a complete data set of size frequencies for CAIs and chondrules.

  5. Comparing Spreading Resistance Profiling and C-V characterisation to identify defects in silicon sensors

    NASA Astrophysics Data System (ADS)

    Dragicevic, M.; Bergauer, T.; Hrubec, J.; Krammer, M.; Treberspurg, W.; Valentan, M.

    2013-02-01

    The quality and functionality of a silicon sensor strongly depends on the effective doping concentration of the active silicon bulk. The creation of additional defects, by certain steps in the production or through irradiation in a particle beam, can heavily influence its performance. Several methods exist to characterise the bulk material for a silicon sensor. C-V characterisation is a widely implemented, non-destructive method to extract the depth profile of Neff. A technique which is rarely used at laboratories developing silicon sensors is Spreading Resistance Profiling (SRP) which directly measures the resistivity of the silicon. We will show, that a comparison of measurements from these two methods can yield important information on the defect concentration in the bulk of the silicon. To demonstrate the technique, we investigated a sensor material where the active region was reduced using a deep diffusion process which is assumed to create additional defects in the bulk.

  6. Use of nootkatone as a senescence indicator for Rouge La Toma Cv. grapefruit (Citrus paradisi Macf.).

    PubMed

    Biolatto, Andrea; Sancho, Ana M; Cantet, Rodolfo J C; Güemes, Daniel R; Pensel, Norma A

    2002-08-14

    The objective of this research was to study the usefulness of nootkatone as a senescence indicator for Rouge La Toma cv. grapefruit (Citrus paradisi Macf.), simulating different treatments that included the normal postharvest handling of citrus fruits: temperature conditioning, cold storage, shipment periods to overseas markets such as Japan and the U.S., marketing conditions, and storage at nonchilling temperature (control treatments). The highest nootkatone levels, determined by GLC-MS analyses, were detected in fruits subjected to control treatments. No significant differences were observed in nootkatone levels between treatments either with or without temperature conditioning prior to the start of the cold storage. Levels of nootkatone increased throughout time for all assayed treatments. The linear regressions of nootkatone levels showed correlation coefficients of 0.80 and 0.83 with storage time (29 and 42 days, respectively). Therefore, nootkatone appears to be a good indicator of senescence for Rouge La Toma grapefruit. PMID:12166965

  7. Analytic expressions for rate and CV of a type I neuron driven by white gaussian noise.

    PubMed

    Lindner, Benjamin; Longtin, André; Bulsara, Adi

    2003-08-01

    We study the one-dimensional normal form of a saddle-node system under the influence of additive gaussian white noise and a static "bias current" input parameter, a model that can be looked upon as the simplest version of a type I neuron with stochastic input. This is in contrast with the numerous studies devoted to the noise-driven leaky integrate-and-fire neuron. We focus on the firing rate and coefficient of variation (CV) of the interspike interval density, for which scaling relations with respect to the input parameter and noise intensity are derived. Quadrature formulas for rate and CV are numerically evaluated and compared to numerical simulations of the system and to various approximation formulas obtained in different limiting cases of the model. We also show that caution must be used to extend these results to the Theta neuron model with multiplicative gaussian white noise. The correspondence between the first passage time statistics for the saddle-node model and the Theta neuron model is obtained only in the Stratonovich interpretation of the stochastic Theta neuron model, while previous results have focused only on the Ito interpretation. The correct Stratonovich interpretation yields CVs that are still relatively high, although smaller than in the Ito interpretation; it also produces certain qualitative differences, especially at larger noise intensities. Our analysis provides useful relations for assessing the distance to threshold and the level of synaptic noise in real type I neurons from their firing statistics. We also briefly discuss the effect of finite boundaries (finite values of threshold and reset) on the firing statistics. PMID:14511512

  8. The Rosa chinensis cv. Viridiflora Phyllody Phenotype Is Associated with Misexpression of Flower Organ Identity Genes.

    PubMed

    Yan, Huijun; Zhang, Hao; Wang, Qigang; Jian, Hongying; Qiu, Xianqin; Baudino, Sylvie; Just, Jeremy; Raymond, Olivier; Gu, Lianfeng; Wang, Jihua; Bendahmane, Mohammed; Tang, Kaixue

    2016-01-01

    Phyllody is a flower abnormality in which leaf-like structures replace flower organs in all whorls. Here, we investigated the origin and the molecular mechanism of phyllody phenotype in Rosa chinensis cv. Viridiflora, an ancient naturally occurring Chinese mutant cultivar. Reciprocal grafting experiments and microscopy analyses, demonstrated that the phyllody phenotype in Viridiflora is not associated with phytoplasmas infection. Transcriptome comparisons by the mean of RNA-Seq identified 672 up-regulated and 666 down-regulated genes in Viridiflora compared to its closely related genotype R. chinensis cv. Old Blush. A fraction of these genes are putative homologs of genes known to be involved in flower initiation and development. We show that in flower whorl 2 of Viridiflora, a down-regulation of the floral organ identity genes RcPISTILLATA (RcPI), RcAPETALA3 (RcAP3) and RcSEPALLATA3 (RcSEP3), together with an up-regulation of the putative homolog of the gene SUPPRESSOR of OVEREXPRESSION of CONSTANS1 (RcSOC1) are likely at the origin of the loss of petal identity and leaf-like structures formation. In whorl 3 of Viridiflora, ectopic expression of RcAPETALA2 (RcAP2) along with the down regulation of RcPI, RcAP3, and RcSEP3 is associated with loss of stamens identity and leaf-like structures formation. In whorl 4, the ectopic expression of RcAP2 associated with a down-regulation of RcSEP3 and of the C-class gene RcAGAMOUS correlate with loss of pistil identity. The latter also suggested the antagonist effect between the A and C class genes in the rose. Together, these data suggest that modified expression of the ABCE flower organ identity genes is associated with the phyllody phenotype in the rose Viridiflora and that these genes are important for normal flower organs development. PMID:27462328

  9. Chemical evolution of metal in refractory inclusions in CV3 chondrites

    NASA Astrophysics Data System (ADS)

    Campbell, Andrew J.; Simon, Steven B.; Humayun, Munir; Grossman, Lawrence

    2003-09-01

    Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to measure distributions of the siderophile elements V, Fe, Co, Ni, Mo, Ru, Rh, Pd, W, Re, Os, Ir, Pt, and Au in Fremdlinge with a spatial resolution of 15 to 25 μm. A sulfide vein in a refractory inclusion in Allende (CV3-oxidized) is enriched in Rh, Ru, and Os with no detectable Pd, Re, Ir, or Pt, indicating that Rh, Ru, and Os were redistributed by sulfidation of the inclusion, causing fractionation of Re/Os and other siderophile element ratios in Allende CAIs. Fremdlinge in compact Type-A inclusions from Efremovka (CV3-reduced) exhibit subsolidus exsolution into kamacite and taenite and minimal secondary formation of V-magnetite and schreibersite. Siderophile element partitioning between taenite and kamacite is similar to that observed previously in iron meteorites, while preferential incorporation of the light PGEs (Ru, Rh, Pd) relative to Re, Os, Ir, and Pt by schreibersite was observed. Fremdling EM2 (CAI Ef2) has an outer rim of P-free metal that preserves the PGE signature of schreibersite, indicating that EM2 originally had a phosphide rim and lost P to the surrounding inclusion during secondary processing. Most Fremdlinge have chondrite-normalized refractory PGE patterns that are unfractionated, with PGE abundances derived from a small range of condensation temperatures, ˜1480 to 1468 K at P tot = 10 -3 bar. Some Fremdlinge from the same CAI exhibit sloping PGE abundance patterns and Re/Os ratios up to 2 × CI that likely represent mixing of grains that condensed at various temperatures.

  10. The Rosa chinensis cv. Viridiflora Phyllody Phenotype Is Associated with Misexpression of Flower Organ Identity Genes

    PubMed Central

    Yan, Huijun; Zhang, Hao; Wang, Qigang; Jian, Hongying; Qiu, Xianqin; Baudino, Sylvie; Just, Jeremy; Raymond, Olivier; Gu, Lianfeng; Wang, Jihua; Bendahmane, Mohammed; Tang, Kaixue

    2016-01-01

    Phyllody is a flower abnormality in which leaf-like structures replace flower organs in all whorls. Here, we investigated the origin and the molecular mechanism of phyllody phenotype in Rosa chinensis cv. Viridiflora, an ancient naturally occurring Chinese mutant cultivar. Reciprocal grafting experiments and microscopy analyses, demonstrated that the phyllody phenotype in Viridiflora is not associated with phytoplasmas infection. Transcriptome comparisons by the mean of RNA-Seq identified 672 up-regulated and 666 down-regulated genes in Viridiflora compared to its closely related genotype R. chinensis cv. Old Blush. A fraction of these genes are putative homologs of genes known to be involved in flower initiation and development. We show that in flower whorl 2 of Viridiflora, a down-regulation of the floral organ identity genes RcPISTILLATA (RcPI), RcAPETALA3 (RcAP3) and RcSEPALLATA3 (RcSEP3), together with an up-regulation of the putative homolog of the gene SUPPRESSOR of OVEREXPRESSION of CONSTANS1 (RcSOC1) are likely at the origin of the loss of petal identity and leaf-like structures formation. In whorl 3 of Viridiflora, ectopic expression of RcAPETALA2 (RcAP2) along with the down regulation of RcPI, RcAP3, and RcSEP3 is associated with loss of stamens identity and leaf-like structures formation. In whorl 4, the ectopic expression of RcAP2 associated with a down-regulation of RcSEP3 and of the C-class gene RcAGAMOUS correlate with loss of pistil identity. The latter also suggested the antagonist effect between the A and C class genes in the rose. Together, these data suggest that modified expression of the ABCE flower organ identity genes is associated with the phyllody phenotype in the rose Viridiflora and that these genes are important for normal flower organs development. PMID:27462328

  11. Using Multidimensional Rasch Analysis to Validate the Chinese Version of the Motivated Strategies for Learning Questionnaire (MSLQ-CV)

    ERIC Educational Resources Information Center

    Lee, John Chi-Kin; Zhang, Zhonghua; Yin, Hongbiao

    2010-01-01

    This article used the multidimensional random coefficients multinomial logit model to examine the construct validity and detect the substantial differential item functioning (DIF) of the Chinese version of motivated strategies for learning questionnaire (MSLQ-CV). A total of 1,354 Hong Kong junior high school students were administered the…

  12. Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "K...

  13. Confirmation By QTL mapping Of The Malus Robusta (Cv. Robusta 5) derived powdery mildew resistance gene Pl1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Geneva® apple rootstock breeding program has made extensive use of Malus robusta cv. Robusta 5 as a source of resistance to fire blight. Robusta 5 has also been used as the source of powdery mildew resistance by other breeding programs and a single locus Pl1 has been associated with this resist...

  14. Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv. 'Tropic Sun')

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydr...

  15. Water Deficit Effect on Ratio of Seed to Berry Fresh Weight and Berry Weight Uniformity in Winegrape cv. Merlot

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Field-grown grapevines cv. Merlot (Vitis vinifera L.) were differentially irrigated in a randomized block design during two growing seasons to maintain a high or low level of vine water stress between fruit set and harvest. Detached berries from clusters harvested at maturity were individually weig...

  16. Inherited transgene expression of the uidA and bar genes in Lilium longiflorum cv. Nellie White

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The expression of two transgenes, bar and uidA, was studied in Lilium longiflorum cv. Nellie White plants. ‘Nellie White’ had been transformed using the gene gun to bombard with pDM327 that contains the bar-uidA fusion gene under control of the CaMV 35S promoter. PCR analysis confirmed that eight ...

  17. The Parent Interview for Autism-Clinical Version (PIA-CV): A Measure of Behavioral Change for Young Children with Autism.

    ERIC Educational Resources Information Center

    Stone, Wendy L.; Coonrod, Elaine E.; Pozdol, Stacie L.; Turner, Lauren M.

    2003-01-01

    Two studies were conducted to examine the psychometric properties of the Parent Interview for Autism-Clinical Version (PIA-CV) for 58 children (ages 2-5). Results support the utility of the PIA-CV for obtaining ecologically valid information from parents and for measuring behavioral change in young children with autism. (Contains references.)…

  18. Synchronizing the Absolute and Relative Clocks: Pb-Pb and Al-Mg Systematics in CAIs from the Allende and NWA 2364 CV3 Chondrites

    NASA Astrophysics Data System (ADS)

    Bouvier, A.; Wadhwa, M.

    2009-03-01

    A Pb-Pb internal isochron of a type-B CAI from the NWA 2364 CV3 chondrite gives an absolute age of 4568.6 ± 0.2 Ma which contrasts with previous internal Pb-Pb ages of CAIs from Allende and Efremovka. Al-Mg systematics are also reported for CV3 CAIs.

  19. Draft Genome Sequence of Aliiroseovarius crassostreae CV919-312, the Causative Agent of Roseovarius Oyster Disease (Formerly Juvenile Oyster Disease)

    PubMed Central

    Kessner, Linda; Spinard, Edward; Gomez-Chiarri, Marta; Rowley, David C.

    2016-01-01

    Aliiroseovarius crassostreae CV919-312 is a marine alphaproteobacterium and the causative agent of Roseovarius oyster disease. We announce here the draft genome sequence of A. crassostreae CV919-312 and identify potential virulence genes involved in pathogenicity. PMID:26988054

  20. Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan

    PubMed Central

    2010-01-01

    Background We performed an environmental study of viruses infecting the symbiotic single-celled algae of Paramecium bursaria (Paramecium bursaria Chlorella virus, PBCV) in Lake Biwa, the largest lake in Japan. The viruses detected were all Chlorella variabilis virus (CvV = NC64A virus). One of them, designated CvV-BW1, was subjected to further characterization. Results CvV-BW1 formed small plaques and had a linear DNA genome of 370 kb, as judged by pulsed-field gel electrophoresis. Restriction analysis indicated that CvV-BW1 DNA belongs to group H, one of the most resistant groups among CvV DNAs. Based on a phylogenetic tree constructed using the dnapol gene, CvV was classified into two clades, A and B. CvV-BW1 belonged to clade B, in contrast to all previously identified virus strains of group H that belonged to clade A. Conclusions We conclude that CvV-BW1 composes a distinct species within C. variabilis virus. PMID:20831832

  1. Draft Genome Sequence of Aliiroseovarius crassostreae CV919-312, the Causative Agent of Roseovarius Oyster Disease (Formerly Juvenile Oyster Disease).

    PubMed

    Kessner, Linda; Spinard, Edward; Gomez-Chiarri, Marta; Rowley, David C; Nelson, David R

    2016-01-01

    Aliiroseovarius crassostreae CV919-312 is a marine alphaproteobacterium and the causative agent of Roseovarius oyster disease. We announce here the draft genome sequence of A. crassostreae CV919-312 and identify potential virulence genes involved in pathogenicity. PMID:26988054

  2. Abundance, major element composition and size of components and matrix in CV, CO and Acfer 094 chondrites

    NASA Astrophysics Data System (ADS)

    Ebel, Denton S.; Brunner, Chelsea; Konrad, Kevin; Leftwich, Kristin; Erb, Isabelle; Lu, Muzhou; Rodriguez, Hugo; Crapster-Pregont, Ellen J.; Friedrich, Jon M.; Weisberg, Michael K.

    2016-01-01

    The relative abundances and chemical compositions of the macroscopic components or "inclusions" (chondrules and refractory inclusions) and fine-grained mineral matrix in chondritic meteorites provide constraints on astrophysical theories of inclusion formation and chondrite accretion. We present new techniques for analysis of low count/pixel Si, Mg, Ca, Al, Ti and Fe X-ray intensity maps of rock sections, and apply them to large areas of CO and CV chondrites, and the ungrouped Acfer 094 chondrite. For many thousands of manually segmented and type-identified inclusions, we are able to assess, pixel-by-pixel, the major element content of each inclusion. We quantify the total fraction of refractory elements accounted for by various types of inclusion and matrix. Among CO chondrites, both matrix and inclusion Mg/Si ratios approach the solar (and bulk CO) ratio with increasing petrologic grade, but Si remains enriched in inclusions relative to matrix. The oxidized CV chondrites with higher matrix/inclusion ratios exhibit more severe aqueous alteration (oxidation), and their excess matrix accounts for their higher porosity relative to reduced CV chondrites. Porosity could accommodate an original ice component of matrix as the direct cause of local alteration of oxidized CV chondrites. We confirm that major element abundances among inclusions differ greatly, across a wide range of CO and CV chondrites. These abundances in all cases add up to near-chondritic (solar) bulk abundance ratios in these chondrites, despite wide variations in matrix/inclusion ratios and inclusion sizes: chondrite components are complementary. This complementarity provides a robust meteoritic constraint for astrophysical disk models.

  3. The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface.

    PubMed

    Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A; Bianchet, Mario A; Wang, Lai-Xi; Wilson, Iain B H; Vasta, Gerardo R

    2013-08-23

    The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is "hijacked" by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified β-integrin and dominin as CvGal1 "self"-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to β-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

  4. The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface*

    PubMed Central

    Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N.; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A.; Bianchet, Mario A.; Wang, Lai-Xi; Wilson, Iain B. H.; Vasta, Gerardo R.

    2013-01-01

    The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is “hijacked” by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288,) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified β-integrin and dominin as CvGal1 “self”-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to β-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

  5. Chondrules in CK carbonaceous chondrites and thermal history of the CV-CK parent body

    NASA Astrophysics Data System (ADS)

    Chaumard, NoëL.; Devouard, Bertrand

    2016-03-01

    CK chondrites are the only group of carbonaceous chondrites with petrologic types ranging from 3 to 6. It is commonly reported than ~15 vol% of CK4-6 samples are composed of chondrules. The modal abundance of chondrules estimated here for 18 CK3-6 (including five CK3s) ranges from zero (totally recrystallized) to 50.5%. Although almost all chemically re-equilibrated with the host matrix, we recognized in CK3s and Tanezrouft (Tnz) 057 (CK4) up to 85% of chondrules as former type I chondrules. Mean diameters of chondrules range from 0.22 to 1.05 mm for Karoonda (CK4) and Tnz 057 (CK4), respectively. Up to ~60% of chondrules in CK3-4 are surrounded by igneous rims (from ~20 μm to 2 mm width). Zoned olivines were found in unequilibrated chondrules from DaG 431 (CK3-an), NWA 4724 (CK3.8), NWA 4423 (CK3.9), and Tnz 057 (CK4). We modeled Fe/Mg interdiffusion profiles measured in zoned olivines to evaluate the peak metamorphic temperatures and time scales of the CK parent body metamorphism, and proposed a two-stage diffusion process in order to account for the position of inflection points situated within chondrules. Time scales inferred from Fe/Mg interdiffusion in olivine from unequilibrated chondrules are on the order of tens to a hundred thousand years (from 50 to 70,000 years for peak metamorphic temperatures of 1140 and 920 K, respectively). These durations are longer than what is commonly accepted for shock metamorphism and shorter than what is required for nuclide decay. Using the concept of a continuous CV-CK metamorphic series, which is reinforced by this study, we estimated peak metamorphic temperatures <850 K for CV, 850-920 K for CK3, and 920-1140 K for CK4-6 chondrites considering a duration of 70,000 years.

  6. Compound ultrarefractory CAI-bearing inclusions from CV3 carbonaceous chondrites

    NASA Astrophysics Data System (ADS)

    Ivanova, Marina A.; Krot, Alexander N.; Nagashima, Kazuhide; MacPherson, Glenn J.

    2012-12-01

    Abstract-Two compound calcium-aluminum-rich inclusions (CAIs), 3N from the oxidized <span class="hlt">CV</span> chondrite Northwest Africa (NWA) 3118 and 33E from the reduced <span class="hlt">CV</span> chondrite Efremovka, contain ultrarefractory (UR) inclusions. 3N is a forsterite-bearing type B (FoB) CAI that encloses UR inclusion 3N-24 composed of Zr,Sc,Y-rich oxides, Y-rich perovskite, and Zr,Sc-rich Al,Ti-diopside. 33E contains a fluffy type A (FTA) CAI and UR CAI 33E-1, surrounded by Wark-Lovering rim layers of spinel, Al-diopside, and forsterite, and a common forsterite-rich accretionary rim. 33E-1 is composed of Zr,Sc,Y-rich oxides, Y-rich perovskite, Zr,Sc,Y-rich pyroxenes (Al,Ti-diopside, Sc-rich pyroxene), and gehlenite. 3N-24's UR oxides and Zr,Sc-rich Al,Ti-diopsides are 16O-poor (Δ17O approximately -2‰ to -5‰). Spinel in 3N-24 and spinel and Al-diopside in the FoB CAI are 16O-rich (Δ17O approximately -23 ± 2‰). 33E-1's UR oxides and Zr,Sc-rich Al,Ti-diopsides are 16O-depleted (Δ17O approximately -2‰ to -5‰) vs. Al,Ti-diopside of the FTA CAI and spinel (Δ17O approximately -23 ± 2‰), and Wark-Lovering rim Al,Ti-diopside (Δ17O approximately -7‰ to -19‰). We infer that the inclusions experienced multistage formation in nebular regions with different oxygen-isotope compositions. 3N-24 and 33E-1's precursors formed by evaporation/condensation above 1600 °C. 3N and 33E's precursors formed by condensation and melting (3N only) at significantly lower temperatures. 3N-24 and 3N's precursors aggregated into a compound object and experienced partial melting and thermal annealing. 33E-1 and 33E avoided melting prior to and after aggregation. They acquired Wark-Lovering and common forsterite-rich accretionary rims, probably by condensation, followed by thermal annealing. We suggest 3N-24 and 33E-1 originated in a 16O-rich gaseous reservoir and subsequently experienced isotope exchange in a 16O-poor gaseous reservoir. Mechanism and timing of oxygen-isotope exchange remain</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/26358178','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/26358178"><span id="translatedtitle">Structural Insight into Cell Wall Architecture of Micanthus sinensis <span class="hlt">cv</span>. using Correlative Microscopy Approaches.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Ma, Jianfeng; Lv, Xunli; Yang, Shumin; Tian, Genlin; Liu, Xing'e</p> <p>2015-10-01</p> <p>Structural organization of the plant cell wall is a key parameter for understanding anisotropic plant growth and mechanical behavior. Four imaging platforms were used to investigate the cell wall architecture of Miscanthus sinensis <span class="hlt">cv</span>. internode tissue. Using transmission electron microscopy with potassium permanganate, we found a great degree of inhomogeneity in the layering structure (4-9 layers) of the sclerenchymatic fiber (Sf). However, the xylem vessel showed a single layer. Atomic force microscopy images revealed that the cellulose microfibrils (Mfs) deposited in the primary wall of the protoxylem vessel (Pxv) were disordered, while the secondary wall was composed of Mfs oriented in parallel in the cross and longitudinal section. Furthermore, Raman spectroscopy images indicated no variation in the Mf orientation of Pxv and the Mfs in Pxv were oriented more perpendicular to the cell axis than that of Sfs. Based on the integrated results, we have proposed an architectural model of Pxv composed of two layers: an outermost primary wall composed of a meshwork of Mfs and inner secondary wall containing parallel Mfs. This proposed model will support future ultrastructural analysis of plant cell walls in heterogeneous tissues, an area of increasing scientific interest particularly for liquid biofuel processing. PMID:26358178</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/21212957','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/21212957"><span id="translatedtitle">Highly efficient Agrobacterium-mediated transformation of banana <span class="hlt">cv</span>. Rasthali (AAB) via sonication and vacuum infiltration.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Subramanyam, Kondeti; Subramanyam, Koona; Sailaja, K V; Srinivasulu, M; Lakshmidevi, K</p> <p>2011-03-01</p> <p>A reproducible and efficient transformation method was developed for the banana <span class="hlt">cv</span>. Rasthali (AAB) via Agrobacterium-mediated genetic transformation of suckers. Three-month-old banana suckers were used as explant and three Agrobacterium tumefaciens strains (EHA105, EHA101, and LBA4404) harboring the binary vector pCAMBIA1301 were used in the co-cultivation. The banana suckers were sonicated and vacuum infiltered with each of the three A. tumefaciens strains and co-cultivated in the medium containing different concentrations of acetosyringone for 3 days. The transformed shoots were selected in 30 mg/l hygromycin-containing selection medium and rooted in rooting medium containing 1 mg/l IBA and 30 mg/l hygromycin. The presence and integration of the hpt II and gus genes into the banana genome were confirmed by GUS histochemical assay, polymerase chain reaction, and southern hybridization. Among the different combinations tested, high transformation efficiency (39.4 ± 0.5% GUS positive shoots) was obtained when suckers were sonicated and vacuum infiltered for 6 min with A. tumefaciens EHA105 in presence of 50 μM acetosyringone followed by co-cultivation in 50 μM acetosyringone-containing medium for 3 days. These results suggest that an efficient Agrobacterium-mediated transformation protocol for stable integration of foreign genes into banana has been developed and that this transformation system could be useful for future studies on transferring economically important genes into banana. PMID:21212957</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1067057','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1067057"><span id="translatedtitle">Promotion of Flowering in Brassica campestris L. <span class="hlt">cv</span> Ceres by Sucrose</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Friend, Douglas J. C.; Bodson, Monique; Bernier, Georges</p> <p>1984-01-01</p> <p>Flower initiation of the quantitative long-day plant Brassica campestris <span class="hlt">cv</span> Ceres was earlier and at a lower final leaf number when sucrose was added to the medium in which plants were grown in sterile culture. The optimal concentration of sucrose was 40 to 80 millimolar. This flower-promoting effect of sucrose was not osmotic, as mannitol, sodium chloride, and polyethylene glycol were not effective at equal osmotic potentials. Seedlings grown heterotrophically after treatment with 4-chloro-5-(dimethylamino)-2-phenyl-3-(2H)-pyridazinone to prevent chlorophyll accumulation were also induced to form flower primordia earlier as the sucrose concentration in the medium was increased up to 80 millimolar. Inclusion of 4 millimolar sodium nitrate in the culture medium of green plants did not reduce the flower-promoting effects of sucrose but delayed initiation in plants grown without added sucrose. Removal of CO2 during a single main or supplementary light period, or both, greatly reduced flower initiation. It is concluded that sucrose may be an important controlling factor determining floral initiation in Brassica. PMID:16663739</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/25945643','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/25945643"><span id="translatedtitle">Priming and temperature limits for germination of dispersal units of Urochloa brizantha (Stapf) Webster <span class="hlt">cv</span>. basilisk.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Nakao, E A; Cardoso, V J M</p> <p>2015-01-01</p> <p>The objective of this study was to evaluate the effect of priming treatments on the upper and lower thermal limits for germination of Urochloa brizantha <span class="hlt">cv</span>. basilisk, and testing the hypothesis that pré-imbibition affect thermal parameters of the germination. Pre-imbibed seeds both in distilled water (0 MPa) and PEG 6000 solution (-0.5 MPa) were put to germinate in different temperatures. It is suggested that U. brizantha seeds have low response to priming when they were placed to germinate in medium where water is not limiting. The response of U. brizantha seeds to priming is dependent on the temperature and water potential conditions at which the seeds are pre-imbibed, as well as on the germination temperature. The optimum temperature for germination of U. brizantha shift toward warmer temperatures in primed seeds. Priming effect was more pronounced at temperatures closer to the upper and lower limit for germination, but probably that response cannot be accounted for changes in the thermal time constant (θT(g)) and ceiling temperature (Tc(g)). Otherwise, a decrease in the base temperature (Tb) was observed in primed seeds, suggesting that the Tb distribution in U. brizantha seeds is influenced by priming. PMID:25945643</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.osti.gov/scitech/biblio/5640533','SCIGOV-STC'); return false;" href="http://www.osti.gov/scitech/biblio/5640533"><span id="translatedtitle">Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota <span class="hlt">cv</span>. Danvers</span></a></p> <p><a target="_blank" href="http://www.osti.gov/scitech">SciTech Connect</a></p> <p>Wenling Hsieh; Sze, Heven )</p> <p>1991-05-01</p> <p>The transport properties of Ca-pumping ATPases from carrot (Daucus carota <span class="hlt">cv</span>. Danvers) tissue culture cells were studied. ATP dependent Ca transport in vesicles that comigrated with an ER marker, was stimulated 3-4 fold by calmodulin. Cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum Ca-ATPase) partially inhibited oxalate-stimulated Ca transport activity; however, it had little or not effect on calmodulin-stimulated Ca uptake. The results suggested the presence of two types of Ca ATPases, and ER- and a plasma membrane-type. Incubation of membranes with (gamma{sup 32}P)ATP resulted in the formation of a single acyl ({sup 32}P) phosphoprotein of 120 kDa. Formation of this phosphoprotein was dependent on Ca, and enhanced by La {sup 3+}, characteristic of the plasma membrane CaATPase. Acidic phospholipids, like phosphatidylserine, stimulated Ca transport, similar to their effect on the erythrocyte plasma membrane CaATPase. These results would indicate that the calmodulin-stimulated Ca transport originated in large part from a plasma membrane-type Ca pump of 120 kDa.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/27595066','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/27595066"><span id="translatedtitle">Resequencing of Curcuma longa L. <span class="hlt">cv</span>. Kedaram through transcriptome profiling reveals various novel transcripts.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Sahoo, Ambika; Jena, Sudipta; Sahoo, Suprava; Nayak, Sanghamitra; Kar, Basudeba</p> <p>2016-09-01</p> <p>Curcuma longa L. (Turmeric), of the family Zingiberaceae, is one of the economically as well as medicinally important plant species. It is a sterile, polyploid and vegetatively propagated spice crop cultivated usually in Southeast Asia. In the current study, we carried out re-sequencing through transcriptome profiling of Curcuma longa <span class="hlt">cv</span>. Kedaram (Cl_Ked_6). We acquired a total of 1 GB raw data by resequencing through paired-end sequencing using Nextseq 500 platform. The raw data obtained in this study can be accessible in NCBI database with accession number of SRR3928562 with bioproject accession number PRJNA324755. Cufflinks-2.2.1 tool was used for transcriptome assembly which resulted in 39,554 numbers of transcripts. The transcript length ranged from 76 to 15,568, having N50 value of 1221 and median transcript length of 860. We annotated the transcripts using multiple databases. This data will be beneficial for studying sequence variations particularly SNPs between cultivars of turmeric towards authentic identification and discovery of novel functional transcripts in Kedaram. PMID:27595066</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.osti.gov/scitech/biblio/22357129','SCIGOV-STC'); return false;" href="http://www.osti.gov/scitech/biblio/22357129"><span id="translatedtitle">The BANANA project. V. Misaligned and precessing stellar rotation axes in <span class="hlt">CV</span> Velorum</span></a></p> <p><a target="_blank" href="http://www.osti.gov/scitech">SciTech Connect</a></p> <p>Albrecht, Simon; Winn, Joshua N.; Triaud, Amaury; Torres, Guillermo; Fabrycky, Daniel C.; Setiawan, Johny; Gillon, Michaël; Jehin, Emmanuel; Queloz, Didier; Snellen, Ignas; Eggleton, Peter</p> <p>2014-04-20</p> <p>As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary <span class="hlt">CV</span> Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of β{sub p} = –52° ± 6° and β{sub s} = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i {sub *}) over the last 30 yr, leading to a model in which the primary star's obliquity is ≈65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i {sub *}. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/26202971','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/26202971"><span id="translatedtitle">G-quadruplex formation in double strand DNA probed by NMM and <span class="hlt">CV</span> fluorescence.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Kreig, Alex; Calvert, Jacob; Sanoica, Janet; Cullum, Emily; Tipanna, Ramreddy; Myong, Sua</p> <p>2015-09-18</p> <p>G-quadruplexes (GQs) are alternative DNA secondary structures that can form throughout the human genome and control the replication and transcription of important regulatory genes. Here, we established an ensemble fluorescence assay by employing two GQ-interacting compounds, N-methyl mesoporphyrin IX (NMM) and Crystal Violet (<span class="hlt">CV</span>). This enables quantitative measurement of the GQ folding propensity and conformation specificity in both single strand (ss) and double strand (ds) DNA. Our GQ mapping indicates that the likelihood of GQ formation is substantially diminished in dsDNA, likely due to the competition from the Watson-Crick base pairing. Unlike GQ folding sequence in ssDNA which forms both parallel and antiparallel GQs, dsDNA displays only parallel folding. Additionally, we employed single molecule FRET to obtain a direct quantitation of stably formed-, weakly folded and unfolded GQ conformations. The findings of this study and the method developed here will enable identifying and classifying potential GQ-forming sequences in human genome. PMID:26202971</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/25796713','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/25796713"><span id="translatedtitle">[In vitro and in vivo effects of mango pulp (Mangifera indica <span class="hlt">cv</span>. Azucar) in colon carcinogenesis].</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Corrales-Bernal, Andrea; Amparo Urango, Luz; Rojano, Benjamín; Maldonado, Maria Elena</p> <p>2014-03-01</p> <p>Mango pulp contains ascorbic acid, carotenoids, polyphenols, terpenoids and fiber which are healthy and could protect against colon cancer. The aim of this study was to evaluate the antiproliferative and preventive capacity of an aqueous extract of Mangifera indica <span class="hlt">cv</span>. Azúcar on a human colon adenocarcinoma cell line (SW480) and in a rodent model of colorectal cancer, respectively. The content of total phenolics, flavonoids and carotenoids were also analyzed in the extract. SW480 cell growth was inhibited in a dose and time dependent manner by 22.3% after a 72h exposure to the extract (200 µg/ mL). Colon carcinogenesis was initiated in Balb/c mice by two intra-peritoneal injections of azoxymethane (AOM) at the third and fourth week of giving mango in drinking water (0.3%, 0.6%, 1.25%). After 10 weeks of treatment, in the colon of mice receiving 0.3% mango, aberrant crypt foci formation was inhibited more than 60% (p=0,05) and the inhibition was dose-dependent when compared with controls receiving water. These results show that mango pulp, a natural food, non toxic, part of human being diet, contains bioactive compounds able to reduce growth of tumor cells and to prevent the appearance of precancerous lesions in colon during carcinogenesis initiation. PMID:25796713</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/26471628','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/26471628"><span id="translatedtitle">Phenolics from strawberry <span class="hlt">cv</span>. Falandi and their antioxidant and α-glucosidase inhibitory activities.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Yang, Dan; Xie, Haihui; Jiang, Yueming; Wei, Xiaoyi</p> <p>2016-03-01</p> <p>Three new phenolic glucosides, falandiosides A (1), B (2), and C (6) were isolated from strawberry (Fragaria×ananassa Duch.) <span class="hlt">cv</span>. Falandi fruit, together with three flavone glucuronides (3-5), eleven lignan glycosides (12-22), and five others. Their structures were determined by spectroscopic methods. All the known phenolics were reported from strawberry for the first time. They were evaluated for antioxidant and α-glucosidase inhibitory activities. Three new and fifteen known phenolics showed potent 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity with IC50 values of 22.50-4.28μM in comparison to l-ascorbic acid (14.21μM). Quercetin 3-(6-methylglucuronide) (4), (+)-isolariciresinol 9'-glucoside (12), and (-)-isolariciresinol 9'-glucoside (13) were active in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Moreover, compounds 12 and 13 had moderate ferric reducing antioxidant power (FRAP) values. Further, two new and seven known phenolics exhibited more potent α-glucosidase inhibitory activity with IC50 values of 537.43-25.39μM than acarbose (619.94μM). PMID:26471628</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/23561174','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/23561174"><span id="translatedtitle">Characterisation of chlorophyll oxidation mediated by peroxidative activity in olives (Olea europaea L.) <span class="hlt">cv</span>. Hojiblanca.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Vergara-Domínguez, Honorio; Roca, María; Gandul-Rojas, Beatriz</p> <p>2013-08-15</p> <p>The oxidation of chlorophyll a (chl a) catalysed by peroxidase (POD) from mesocarp of the olive fruit (Olea europaea L., <span class="hlt">cv</span> Hojiblanca) in the presence of H2O2 and 2,4-dichlorophenol (2,4-DCP), is characterised via the individualised quantification of the products of the enzymatic reaction using a new methodology of HPLC-UV spectrometry. This innovation has allowed the discovery that, in addition to 13(2) OH chl a and 15(1) OH lactone chl a, which are the first products of POD on chl a, the reaction process sequentially creates another series of oxidised chlorophyll derivatives which have not been previously described. Their origins have been linked to POD activity in the presence of 2,4-DCP. Likewise, a study of the effect of the concentration of the various cosubstrates on the POD reaction rate demonstrated that the correct establishment of the relative concentrations of the same ([H2O2]/[2,4-DCP]/[Chl]=1:3:0.02) is crucial to explaining inhibition effects by substrates and carrying out optimum measurements. Therefore, new essential parameters for the determination of POD activity on a chlorophyll substrate are established. PMID:23561174</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3419424','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3419424"><span id="translatedtitle">Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. <span class="hlt">Cv</span>. MRQ 74)</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Taha, Rosna Mat; Saleh, Azani; Mahmad, Noraini; Hasbullah, Nor Azlina; Mohajer, Sadegh</p> <p>2012-01-01</p> <p>Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5 mm microshoots of Oryza sativa L. <span class="hlt">Cv</span>. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1 mg/L BAP, and 0.1 mg/L α-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1 mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds. PMID:22919338</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.osti.gov/scitech/biblio/5626595','SCIGOV-STC'); return false;" href="http://www.osti.gov/scitech/biblio/5626595"><span id="translatedtitle">Measurement of heme efflux and heme content in isolated developing chloroplasts. [Cucumis sativus, <span class="hlt">cv</span>. Sumter</span></a></p> <p><a target="_blank" href="http://www.osti.gov/scitech">SciTech Connect</a></p> <p>Thomas, J.; Weinstein, J.D. )</p> <p>1990-11-01</p> <p>Hemes destined for cytosolic hemoproteins must originate in one of the cellular compartments which have the capacity for heme synthesis, namely the chloroplast or the mitochondria. Since developing chloroplasts from greening cucumber (Cucumis sativus, <span class="hlt">cv</span>. Sumter) cotyledons are known to contain complete heme and chlorophyll biosynthetic pathways, they were tested for their capacity export hemes. Picomole quantities of heme were measured by reconstitution of the heme with apo-peroxidase and subsequent determination of peroxidase activity. The assay method was sensitive (as little as 0.7 picomole of heme could be detected in a volume of 100 microliters) and was linear with heme concentration. When intact plastids were incubated with apo-peroxidase, a steady-state rate of efflux between 0.12 and 0.45 picomole heme/minute/milligram plastid protein was measured. The efflux rate was not due to plastid breakage and could be enhanced by incubating with the heme precursor, {delta}-aminolevulinic acid. Cold acetone extraction removed 47 {plus minus} 17 picomoles heme/milligram plastid protein from the total b-type heme pool in the chloroplasts (166 {plus minus} 9 picomoles heme/milligram protein, by acid-acetone extraction). The reconstitution technique provided a similar estimate of readily exchangeable heme in the plastid, 37 {plus minus} 8 picomoles heme/milligram protein (or 6 micromolar in the plastids). These values may be indicative of a free heme pool which exists in the chloroplast.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/25160909','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/25160909"><span id="translatedtitle">Characterization of Fusarium wilt resistant somaclonal variants of banana <span class="hlt">cv</span>. Rasthali by cDNA-RAPD.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Ghag, Siddhesh B; Shekhawat, Upendra K S; Ganapathi, Thumballi R</p> <p>2014-12-01</p> <p>Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is counted among the most destructive diseases of crop plants in India. In the absence of any credible control measure to manage this disease, development of resistant cultivars is the best option. Somaclonal variations arising out of long term in vitro culture of plant tissues is an important source of genetic variability and the selection of somaclones having desired characteristics is a promising strategy to develop plants with improved characters. In the present study, we isolated a group of somaclonal variants of banana <span class="hlt">cv</span>. Rasthali which showed efficient resistance towards Foc race 1 infection in repeated bioassays. cDNA-RAPD methodology using 96 decamer primers was used to characterize these somaclonal variants. Among the four differentially amplified bands obtained, one mapping to the coding region of a lipoxygenase gene was confirmed to be down regulated in the somaclones as compared to controls by real-time quantitative RT-PCR. Our results correlated well with earlier studies with lipoxygenase mutants in maize wherein reduced expression of lipoxygenase led to enhanced resistance towards Fusarium infection. PMID:25160909</p> </li> </ol> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_7");'>7</a></li> <li><a href="#" onclick='return showDiv("page_8");'>8</a></li> <li class="active"><span>9</span></li> <li><a href="#" onclick='return showDiv("page_10");'>10</a></li> <li><a href="#" onclick='return showDiv("page_11");'>11</a></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div><!-- col-sm-12 --> </div><!-- row --> </div><!-- page_9 --> <div id="page_10" class="hiddenDiv"> <div class="row"> <div class="col-sm-12"> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_8");'>8</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li class="active"><span>10</span></li> <li><a href="#" onclick='return showDiv("page_11");'>11</a></li> <li><a href="#" onclick='return showDiv("page_12");'>12</a></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div> </div> <div class="row"> <div class="col-sm-12"> <ol class="result-class" start="181"> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/25053073','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/25053073"><span id="translatedtitle">Acylated anthocyanins from sprouts of Raphanus sativus <span class="hlt">cv</span>. Sango: isolation, structure elucidation and antioxidant activity.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Matera, Riccardo; Gabbanini, Simone; Berretti, Serena; Amorati, Riccardo; De Nicola, Gina Rosalinda; Iori, Renato; Valgimigli, Luca</p> <p>2015-01-01</p> <p>Little is known on structure-activity relationships of antioxidant anthocyanins. Raphanus sativus <span class="hlt">cv</span> Sango sprouts are among the richest sources (270 mg/100 g fresh weight). We isolated from sprouts' juice 9 acylated anthocyanins, including 4 new compounds. All comprise a cyanidin core bearing 3-4 glucose units, multiply acylated with malonic and phenolic acids (ferulic and sinapic). All compounds were equally effective in inhibiting the autoxidation of linoleic acid in aqueous micelles, with rate constant for trapping peroxyl radicals kinh=(3.8 ± 0.7) × 10(4)M(-1)s(-1) at 37 °C. In acetonitrile solution kinh varied with acylation: (0.9-2.1) × 10(5)M(-1)s(-1) at 30 °C. Each molecule trapped a number n of peroxyl radicals ranging from 4 to 7. Anthocyanins bearing sinapic acid were more effective than those bearing the ferulic moiety. Under identical settings, deacylated cyanin, ferulic and sinapic acids had kinh of 0.4 × 10(5), 0.3 × 10(5) and 1.6 × 10(5)M(-1)s(-1) respectively, with n ranging 2-3. Results show the major role of acylation on antioxidant performance. PMID:25053073</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1075142','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1075142"><span id="translatedtitle">Regulation of Cytokinin Oxidase Activity in Callus Tissues of Phaseolus vulgaris L. <span class="hlt">cv</span> Great Northern 1</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Chatfield, J. Mark; Armstrong, Donald J.</p> <p>1986-01-01</p> <p>The regulation of cytokinin oxidase activity in callus tissues of Phaseolus vulgaris L. <span class="hlt">cv</span> Great Northern has been examined using an assay based on the oxidation of N6-(Δ2-isopentenyl)adenine-8-14C (i6 Ade-8-14C) to adenine. Solutions of exogenous cytokinins applied directly to the surface of the callus tissues induced relatively rapid increases in cytokinin oxidase activity. The increase in activity was detectable after 1 hour and continued for about 8 hours, reaching values two- to three-fold higher than the controls. The cytokinin-induced increase in cytokinin oxidase activity was inhibited in tissues pretreated with cordycepin or cycloheximide, suggesting that RNA and protein synthesis may be required for the response. Rifampicin and chloramphenicol, at concentrations that inhibited the growth of Great Northern callus tissues, were ineffective in inhibiting the increase in activity. All cytokinin-active compounds tested, including both substrates and nonsubstrates of cytokinin oxidase, were effective in inducing elevated levels of the enzyme in Great Northern callus tissue. The cytokinin-active urea derivative, Thidiazuron, was as effective as any adenine derivative in inducing this response. The addition of Thidiazuron to the reaction volumes used to assay cytokinin oxidase activity resulted in a marked inhibition of the degradation of the labeled i6 Ade-8-14C substrate. On the basis of this result, it is possible that Thidiazuron may serve as a substrate for cytokinin oxidase, but other mechanisms of inhibition have not yet been excluded. PMID:16664650</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=19840030934&hterms=aluminum+inclusions&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D90%26Ntt%3Daluminum%2Binclusions','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=19840030934&hterms=aluminum+inclusions&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D90%26Ntt%3Daluminum%2Binclusions"><span id="translatedtitle">Mineralogy and petrology of chondrules and inclusions in the Mokoia <span class="hlt">CV</span>3 chondrite</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Cohen, R. E.; Kornacki, A. S.; Wood, J. A.</p> <p>1983-01-01</p> <p>The inclusions and chondrules of the Mokoia <span class="hlt">CV</span>3 chondrite are studied systematically and compared with those in the Allende meteorite. Five polished thin sections of Mokoia were examined by optical microscopy, backscattered scanning electron microscopy, and electronprobe microanalysis, and objects greater than about 100 microns in apparent diameter were measured and classified petrographically. Three major types of olivine chondrules are distinguished: igneous chondrules, which evidently crystallized from droplets of silicate melt; recrystallized chondrules, apparently metamorphosed at relatively high temperatures; and accretional aggregates, which are probably fragments of igneous chondrules. Refractory inclusions in Mokoia are generally similar to those found in Allende, although Mokoia inclusions include phyllosilicates rather than feldspathoids and melilite-rich Ca, Al-rich inclusions (CAIs) are more abundant in Allende. The small, fine-grained CAIs, which are more abundant than coarse-grained CAIs in both meteorites, are observed to represent aggregates of three distinct constituents: concentric objects, chaotic material and inclusion matrix. It is concluded that most of the CAIs probably formed during metamorphism, partial melting, and incomplete distillation of primitive dust aggregates heated in the solar nebula.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/2012MNRAS.426.1720D','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/2012MNRAS.426.1720D"><span id="translatedtitle">Using MOST to reveal the secrets of the mischievous Wolf-Rayet binary <span class="hlt">CV</span> Ser</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>David-Uraz, Alexandre; Moffat, Anthony F. J.; Chené, André-Nicolas; Rowe, Jason F.; Lange, Nicholas; Guenther, David B.; Kuschnig, Rainer; Matthews, Jaymie M.; Rucinski, Slavek M.; Sasselov, Dimitar; Weiss, Werner W.</p> <p>2012-11-01</p> <p>The Wolf-Rayet (WR) binary <span class="hlt">CV</span> Serpentis (= WR113, WC8d + O8-9IV) has been a source of mystery since it was shown that its atmospheric eclipses change with time over decades, in addition to its sporadic dust production. The first high-precision time-dependent photometric observations obtained with the Microvariability and Oscillations of STars (MOST) space telescope in 2009 show two consecutive eclipses over the 29-d orbit, with varying depths. A subsequent MOST run in 2010 showed a seemingly asymmetric eclipse profile. In order to help make sense of these observations, parallel optical spectroscopy was obtained from the Mont Megantic Observatory (2009, 2010) and from the Dominion Astrophysical Observatory (2009). Assuming these depth variations are entirely due to electron scattering in a β-law wind, an unprecedented 62 per cent increase in M⊙ is observed over one orbital period. Alternatively, no change in mass-loss rate would be required if a relatively small fraction of the carbon ions in the wind globally recombined and coaggulated to form carbon dust grains. However, it remains a mystery as to how this could occur. There also seems to be evidence for the presence of corotating interaction regions (CIR) in the WR wind: a CIR-like signature is found in the light curves, implying a potential rotation period for the WR star of 1.6 d. Finally, a new circular orbit is derived, along with constraints for the wind collision.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/15054660','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/15054660"><span id="translatedtitle">Rhythmic emission of floral volatiles from Rosa damascena semperflorens <span class="hlt">cv</span>. 'Quatre Saisons'.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Picone, Joanne M; Clery, Robin A; Watanabe, Naoharu; MacTavish, Hazel S; Turnbull, Colin G N</p> <p>2004-07-01</p> <p>The control of rhythmic emission of floral volatiles emitted from Rosa damascena semperflorens <span class="hlt">cv</span>. 'Quatre Saisons' throughout floral development under various light regimes was studied. 2-Phenylethanol was the major volatile emitted in addition to monoterpenols, oxidised monoterpenols, monoterpenes and aromatic compounds. All detected volatiles were emitted rhythmically, with maximum peaks coinciding 8-10 h into a 12-h photoperiod. For some compounds a secondary, nocturnal peak was apparent. The primary and secondary maxima both occurred at approximately 24-h intervals. Rhythms appeared to be regulated endogenously: rhythmic emission continued upon exposure to continuous light or continuous darkness, and a phase shift in emission was induced upon inversion of the photoperiod. Additionally, emission continued after flower excision. A similar profile of free volatiles was stored within the floral tissue, together with glycosidic forms of 2-phenylethanol (>99% beta-D-glucoside), benzyl alcohol, citronellol and geraniol. Regression analysis indicated a significant decrease in glycosylated 2-phenylethanol through the photoperiod. These results suggest that glycosylated volatiles stored within petals may be a source of rhythmically emitted volatiles. PMID:15054660</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/25674504','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/25674504"><span id="translatedtitle">Jasmonates elicit different sets of stilbenes in Vitis vinifera <span class="hlt">cv</span>. Negramaro cell cultures.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Taurino, Marco; Ingrosso, Ilaria; D'amico, Leone; De Domenico, Stefania; Nicoletti, Isabella; Corradini, Danilo; Santino, Angelo; Giovinazzo, Giovanna</p> <p>2015-01-01</p> <p>The plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. A cell suspension culture was developed from calli of grape leaves of Vitis vinifera <span class="hlt">cv</span>. Negramaro in order to study the bioproduction of resveratrol. The effects of a number of secondary plant metabolism elicitors, namely chitosan, methyl jasmonate, jasmonic acid, coronatine, and 12-oxo-phytodienoic acid, were tested on this cell suspension culture. The identification and quantification of stilbenes was achieved with high performance liquid chromatography, with both spectrophotometric and mass spectrometric detection. Of the tested elicitors, methyl jasmonate was the most effective in inducing the biosynthesis of approximately 4 mg g(-1) dry weight (about 60 mg L(-1)) of resveratrol. Conversely, 12-oxo-phytodienoic acid, jasmonic acid, and coronatine were able to trigger the synthesis of approximately 20 mg g(-1) dry weight (200-210 mg L(-1)) of viniferins. Taken together, our results show for the first time different modulatory effects of closely-related jasmonates on stilbene biosynthesis. PMID:25674504</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/26442011','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/26442011"><span id="translatedtitle">Impacts of strigolactone on shoot branching under phosphate starvation in chrysanthemum (Dendranthema grandiflorum <span class="hlt">cv</span>. Jinba).</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Xi, Lin; Wen, Chao; Fang, Shuang; Chen, Xiaoli; Nie, Jing; Chu, JinFang; Yuan, Cunquan; Yan, Cunyu; Ma, Nan; Zhao, Liangjun</p> <p>2015-01-01</p> <p>Chrysanthemum (Dendranthema grandiflorum <span class="hlt">cv</span>. Jinba) shoot branching is determined by bud outgrowth during the vegetative growth stage. The degree of axillary bud outgrowth is highly influenced by environmental conditions, such as nutrient availability. Here, we demonstrated that phosphorus (Pi) starvation significantly reduces axillary bud outgrowth in chrysanthemum. A strigolactone (SL) biosynthesis gene, DgCCD7, was isolated and characterized as an ortholog of MAX3/DAD3/RMS5/D17. By using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS), three putative SLs were identified and levels of all three SLs showed strong increase under Pi starvation conditions. Determinations of the distribution of SLs and regulation of DgCCD7/8 in response to Pi changes in root indicate that SL acts systemically. However, temporal expression patterns of biosynthesis and signaling genes in nodes revealed that Pi starvation causes a local response of SL pathway. Treatment of node segments with or without auxin and Pi revealed that in the absence of exogenous auxin, Pi delayed axillary buds outgrowth and up-regulated local SL pathway genes. These data indicated that an auxin-SL regulatory loop responded to Pi starvation for delaying bud outgrowth locally, root biosynthesized SLs were transported acropetally and functioned in shoot branching inhibition under Pi starvation. We proposed that SLs contributed to chrysanthemum shoot branching control in response to Pi-limiting conditions in a systemic way. PMID:26442011</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/23723045','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/23723045"><span id="translatedtitle">Stable MSAP markers for the distinction of Vitis vinifera <span class="hlt">cv</span> Pinot noir clones.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Ocaña, Juan; Walter, Bernard; Schellenbaum, Paul</p> <p>2013-11-01</p> <p>Grapevine is one of the most economically important fruit crops. Molecular markers have been used to study grapevine diversity. For instance, simple sequence repeats are a powerful tool for identification of grapevine cultivars, while amplified fragment length polymorphisms have shown their usefulness in intra-varietal diversity studies. Other techniques such as sequence-specific amplified polymorphism are based on the presence of mobile elements in the genome, but their detection lies upon their activity. Relevant attention has been drawn toward epigenetic sources of variation. In this study, a set of Vitis vinifera <span class="hlt">cv</span> Pinot noir clones were analyzed using the methylation-sensitive amplified polymorphism technique with isoschizomers MspI and HpaII. Nine out of fourteen selective primer combinations were informative and generated two types of polymorphic fragments which were categorized as "stable" and "unstable." In total, 23 stable fragments were detected and they discriminated 92.5 % of the studied clones. Detected stable polymorphisms were either common to several clones, restricted to a few clones or unique to a single clone. The identification of these stable epigenetic markers will be useful in clonal diversity studies. We highlight the relevance of stable epigenetic variation in V. vinifera clones and analyze at which level these markers could be applicable for the development of forthright techniques for clonal distinction. PMID:23723045</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3317695','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3317695"><span id="translatedtitle">Optimization of Freeze Drying Conditions for Purified Pectinase from Mango (Mangifera indica <span class="hlt">cv</span>. Chokanan) Peel</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd</p> <p>2012-01-01</p> <p>Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica <span class="hlt">cv</span>. Chokanan) peel. The effect of pectinase content (−2.66, 62.66 mg/mL), Arabic gum (−1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. PMID:22489134</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/23692745','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/23692745"><span id="translatedtitle">Nutraceutical properties and polyphenolic profile of berry skin and wine of Vitis vinifera L. (<span class="hlt">cv</span>. Aglianico).</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>De Nisco, Mauro; Manfra, Michele; Bolognese, Adele; Sofo, Adriano; Scopa, Antonio; Tenore, Gian Carlo; Pagano, Francesco; Milite, Ciro; Russo, Maria Teresa</p> <p>2013-10-15</p> <p>Red grapes are rich in phenolics, flavonoids, anthocyanins and resveratrol, all substances which have been suggested as having nutraceutical and health benefits. The berry skin and wine of grape cultivar Vitis vinifera L. (<span class="hlt">cv</span>. Aglianico), grown in Basilicata (Southern Italy) were examined to determinate the presence of the above mentioned compounds as well as to establish the inorganic cation profile. HPLC analysis coupled with LC-ESI/MS/MS detected high contents of total flavonols and anthocyanins in berry skin and wine. The wine made with the same grape used for berry skin assays showed a notable presence of quercetin-3-O-glucoside (39.4% of total flavonols), and malvidin and petunidin derivatives (63.9% and 10.8% of total anthocyanins, respectively). The strong antioxidant ROS-scavenging activity, determined by both DPPH and FRAP assays, and the high resveratrol content confer high sensory characteristics resulted to be associated with positive nutraceutical properties of these grapes and wine. The level of cis-resveratrol was lower than trans-resveratrol in both berry skin and wine reaching 44.1mg/kg and 0.3mg/l, respectively. The cation profile presents low levels of Ca, Cu, K, Fe, Zn and Cd compared to numerous, important red wines, such as Monastrell and Tempranillo. PMID:23692745</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.osti.gov/scitech/biblio/6448700','SCIGOV-STC'); return false;" href="http://www.osti.gov/scitech/biblio/6448700"><span id="translatedtitle">Brassinosteroid stimulation of hypocotyl elongation and wall relaxation in pakchoi (Brassica chinensis <span class="hlt">cv</span> Lei-Choi)</span></a></p> <p><a target="_blank" href="http://www.osti.gov/scitech">SciTech Connect</a></p> <p>Tzannwei Wang; Cosgrove, D.J.; Arteca, R.N. )</p> <p>1993-03-01</p> <p>Hypocotyl elongation of pakchoi (Brassica chinensis <span class="hlt">cv</span> Lei-Choi) was stimulated by applying 300 ng of brassinosteroid (2[alpha],3[alpha],22[beta],23[beta]-tetrahydroxy-24[beta]-methyl-B-homo-7-oxa-5[alpha]-cholestan-6-one, BR) in 1 [mu]L of 50% ethanol to the apex of hypocotyls. BR had its greatest effect on elongation of the apical 3-mm region below the cotyledonary node (75% stimulation) between 6 and 18 h after treatment. Stress/strain (Instron) analysis of this 3-mm region revealed that plastic and elastic components of extension were not significantly different between BR-treated and control seedlings. In pressure-block experiments, the initial rate of relaxation was 2-fold faster in BR-treated plants as compared with controls, whereas after 125 min the total amount of relaxation and the relaxation rate were the same for the two treatments. Osmotic pressure of cell sap expressed from this 3-mm region showed a large decrease (28%) in BR-treated seedlings compared to the controls. The authors conclude that BR stimulates growth in pakchoi by accelerating the biochemical processes that cause wall relaxation, without inducing a large change in wall mechanical properties. 19 refs., 3 figs., 1 tab.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2661133','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2661133"><span id="translatedtitle">Purification and Characterization of a Lectin from Phaseolus vulgaris <span class="hlt">cv</span>. (Anasazi Beans)</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng</p> <p>2009-01-01</p> <p>A lectin has been isolated from seeds of the Phaseolus vulgaris <span class="hlt">cv</span>. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 μM, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 μM. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3755362','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3755362"><span id="translatedtitle">Purification and autolysis of the ficin isoforms from fig (Ficus carica <span class="hlt">cv</span>. Sabz) latex</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader</p> <p>2013-01-01</p> <p>Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees’ latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins’ autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica <span class="hlt">cv</span>. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2008AGUFM.B43C0447S&link_type=ABSTRACT','NASAADS'); return false;" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2008AGUFM.B43C0447S&link_type=ABSTRACT"><span id="translatedtitle">Transpiration, crop coefficient and water use of Olive tree (<span class="hlt">cv</span>. Cordovil) in Southern Portugal</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Santos, F. L.</p> <p>2008-12-01</p> <p>Orchard olive transpiration, soil water status and stomatal response in relation to water deficit were investigated to clarify mechanisms of tree water uptake and stomatal control to improve the irrigation scheduling of low-density olive trees of <span class="hlt">cv</span>. Cordovil grown in typical Mediterranean environment of Southern Portugal. Trees were subject to three irrigation treatments. Treatment A received 100% of crop evapotranspiration by a drip irrigation system, a sustained deficit (SDI)treatment B received 60% of crop evapotranspiration, a regulated deficit(RDI) irrigation treatment C received irrigation water before-flowering and just before pit-hardening, and a Dry-farming treatment. Tree and orchard transpiration and the dynamics of water uptake by roots were estimated from sap flow measurements and water balance technique. Stomatal conductance was modeled from local meteorological variables, measured sap flow and tree canopy variables. Higher than treatment A and B stomatal conductance and the high tree fruit production recommend treatment C as most suitable for scheduling irrigation of olive orchards in wet years of well distributed late summer rainfall. For drier years of no summer and early autumn rains that minimizes available water to extract by roots outside the wet bulb of drip irrigation and for the scarce readily available irrigation water years, as so often occurs in the region, the sustained deficit irrigation (SDI) regime seems a better option. Nonetheless, for years of limited available water resources that preclude sustained deficit irrigation, careful management of the proposed RDI could also allow for efficient use of irrigation water.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=EC95-43199-7&hterms=tires&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D30%26Ntt%3Dtires','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=EC95-43199-7&hterms=tires&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D30%26Ntt%3Dtires"><span id="translatedtitle"><span class="hlt">CV</span>-990 Landing Systems Research Aircraft (LSRA) flight #145 drilling of shuttle tire using Tire Assa</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p></p> <p>1995-01-01</p> <p>Created from a 1/16th model of a German World War II tank, the TAV (Tire Assault Vehicle) was an important safety feature for the Convair 990 Landing System Research Aircraft, which tested space shuttle tires. It was imperative to know the extreme conditions the shuttle tires could tolerate at landing without putting the shuttle and its crew at risk. In addition, the <span class="hlt">CV</span>990 was able to land repeatedly to test the tires. The TAV was built from a kit and modified into a radio controlled, video-equipped machine to drill holes in aircraft test tires that were in imminent danger of exploding because of one or more conditions: high air pressure, high temperatures, and cord wear. An exploding test tire releases energy equivalent to two and one-half sticks of dynamite and can cause severe injuries to anyone within 50 ft. of the explosion, as well as ear injury - possibly permanent hearing loss - to anyone within 100 ft. The degree of danger is also determined by the temperature pressure and cord wear of a test tire. The TAV was developed by David Carrott, a PRC employee under contract to NASA.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/27264640','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/27264640"><span id="translatedtitle">Composition of cuticular waxes coating flag leaf blades and peduncles of Triticum aestivum <span class="hlt">cv</span>. Bethlehem.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Racovita, Radu C; Hen-Avivi, Shelly; Fernandez-Moreno, Josefina-Patricia; Granell, Antonio; Aharoni, Asaph; Jetter, Reinhard</p> <p>2016-10-01</p> <p>The work herein presents comprehensive analyses of the cuticular wax mixtures covering the flag leaf blade and peduncle of bread wheat (Triticum aestivum) <span class="hlt">cv</span>. Bethlehem. Overall, Gas Chromatography-Mass Spectrometry and Flame Ionization Detection revealed a wax coverage of flag leaf blades (16 μg/cm(2)) a third that of peduncles (49 μg/cm(2)). Flag leaf blade wax was dominated by 1-alkanols, while peduncle wax contained primarily β-diketone and hydroxy-β-diketones, thus suggesting differential regulation of the acyl reduction and β-diketone biosynthetic pathways in the two analyzed organs. The characteristic chain length distributions of the various wax compound classes are discussed in light of their individual biosynthetic pathways and biosynthetic relationships between classes. Along with previously reported wheat wax compound classes (fatty acids, 1-alkanols, 1-alkanol esters, aldehydes, alkanes, β-diketone, hydroxy-β-diketones, alkylresorcinols and methyl alkylresorcinols), esters of 2-alkanols and three types of aromatic esters (benzyl, phenethyl and p-hydroxyphenethyl) are also reported. In particular, 2-heptanol esters were identified. Detailed analyses of the isomer distributions within 1-alkanol and 2-alkanol ester homologs revealed distinct patterns of esterified acids and alcohols, suggesting several wax ester synthases with very different substrate preferences in both wheat organs. Terpenoids, including two terpenoid esters, were present only in peduncle wax. PMID:27264640</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4652765','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4652765"><span id="translatedtitle">G-quadruplex formation in double strand DNA probed by NMM and <span class="hlt">CV</span> fluorescence</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Kreig, Alex; Calvert, Jacob; Sanoica, Janet; Cullum, Emily; Tipanna, Ramreddy; Myong, Sua</p> <p>2015-01-01</p> <p>G-quadruplexes (GQs) are alternative DNA secondary structures that can form throughout the human genome and control the replication and transcription of important regulatory genes. Here, we established an ensemble fluorescence assay by employing two GQ-interacting compounds, N-methyl mesoporphyrin IX (NMM) and Crystal Violet (<span class="hlt">CV</span>). This enables quantitative measurement of the GQ folding propensity and conformation specificity in both single strand (ss) and double strand (ds) DNA. Our GQ mapping indicates that the likelihood of GQ formation is substantially diminished in dsDNA, likely due to the competition from the Watson–Crick base pairing. Unlike GQ folding sequence in ssDNA which forms both parallel and antiparallel GQs, dsDNA displays only parallel folding. Additionally, we employed single molecule FRET to obtain a direct quantitation of stably formed-, weakly folded and unfolded GQ conformations. The findings of this study and the method developed here will enable identifying and classifying potential GQ-forming sequences in human genome. PMID:26202971</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2015AIPC.1677i0013H&link_type=ABSTRACT','NASAADS'); return false;" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2015AIPC.1677i0013H&link_type=ABSTRACT"><span id="translatedtitle">Sequence analysis of ORF IV RTBV isolated from tungro infected Oryza sativa L. <span class="hlt">cv</span> Ciherang</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Hastilestari, Bernadetta Rina; Astuti, Dwi; Estiati, Amy; Nugroho, Satya</p> <p>2015-09-01</p> <p>The Effort to increase rice production is often constrained by pest and disease such as Tungro. The Tungro disease is caused by the joint infection with two dissimilar viruses; a bacil-form-DNA virus, the Rice tungro bacilliform virus(RTBV) and the spherical RNA virus, Rice tungro spherical virus (RTSV) and transmitted by Green leafhopper (Nephotettix virescens). The symptom of disease is caused by the presence of RTBV. The genome of RTBV consists of four Open reading frames (ORFs) which encode functional proteins. Of the four, ORF IV is unique because it exists only in RTBV. The most efficient method of generating disease resistance plants is to look for natural sources of resistance genes in wild or germplasm and then transfer the gene and the accompanying resistance in cultivated crop varieties. The aim of this study is, therefore, to isolate and analyze of 1170 bp gene of ORF 4 of Tungro virus isolated from an Indonesian rice cultivar, Ciherang (Oryza sativa L. <span class="hlt">cv</span> Indica). DNA sequencing analysis using BLAST showed 94% similarity with the reference sequence gen bank Acc.M65026.1. The comparisons and mutation analysis of DNA sequences were discussed in this research.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/22489134','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/22489134"><span id="translatedtitle">Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica <span class="hlt">cv</span>. Chokanan) peel.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd</p> <p>2012-01-01</p> <p>Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica <span class="hlt">cv</span>. Chokanan) peel. The effect of pectinase content (-2.66, 62.66 mg/mL), Arabic gum (-1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. PMID:22489134</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/12749079','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/12749079"><span id="translatedtitle">Coumarin inhibits the growth of carrot (Daucus carota L. <span class="hlt">cv</span>. Saint Valery) cells in suspension culture.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Abenavoli, Maria Rosa; Sorgonà, Agostino; Sidari, Maria; Badiani, Maurizio; Fuggi, Amodio</p> <p>2003-03-01</p> <p>We used a carrot (Daucus carota L. <span class="hlt">cv</span>. Saint Valery) cell suspension culture as a simplified model system to study the effects of the allelochemical compound coumarin (1,2 benzopyrone) on cell growth and utilisation of exogenous nitrate, ammonium and carbohydrates. Exposure to micromolar levels of coumarin caused severe inhibition of cell growth starting from the second day of culture onwards. At the same time, the presence of 50 mumol/L coumarin caused accumulation of free amino acids and of ammonium in the cultured cells, and stimulated their glutamine synthetase, glutamate dehydrogenase, glucose-6-phosphate dehydrogenase and phosphoenolpyruvate carboxylase activities. Malate dehydrogenase, on the other hand, was inhibited under the same conditions. These effects were interpreted in terms of the stimulation of protein catabolism and/or interference with protein biosynthesis induced by coumarin. This could have led to a series of compensatory changes in the activities of enzymes linking nitrogen and carbon metabolism. Because coumarin seemed to abolish the exponential phase and to accelerate the onset of the stationary phase of cell growth, we hypothesise that such allelochemical compounds may act in nature as an inhibitor of the cell cycle and/or as a senescence-promoting substance. PMID:12749079</p> </li> </ol> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_8");'>8</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li class="active"><span>10</span></li> <li><a href="#" onclick='return showDiv("page_11");'>11</a></li> <li><a href="#" onclick='return showDiv("page_12");'>12</a></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div><!-- col-sm-12 --> </div><!-- row --> </div><!-- page_10 --> <div id="page_11" class="hiddenDiv"> <div class="row"> <div class="col-sm-12"> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li><a href="#" onclick='return showDiv("page_10");'>10</a></li> <li class="active"><span>11</span></li> <li><a href="#" onclick='return showDiv("page_12");'>12</a></li> <li><a href="#" onclick='return showDiv("page_13");'>13</a></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div> </div> <div class="row"> <div class="col-sm-12"> <ol class="result-class" start="201"> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/26871543','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/26871543"><span id="translatedtitle">Agrobacterium-mediated transformation of Vitis <span class="hlt">Cv</span>. Monastrell suspension-cultured cells: Determination of critical parameters.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Chu, Mingyu; Quiñonero, Carmen; Akdemir, Hülya; Alburquerque, Nuria; Pedreño, María Ángeles; Burgos, Lorenzo</p> <p>2016-05-01</p> <p>Although some works have explored the transformation of differentiated, embryogenic suspension-cultured cells (SCC) to produce transgenic grapevine plants, to our knowledge this is one of the first reports on the efficient transformation of dedifferentiated Vitis vinifera <span class="hlt">cv</span> Monastrell SCC. This protocol has been developed using the sonication-assisted Agrobacterium-mediated transformation (SAAT) method. A construct harboring the selectable nptII and the eyfp/IV2 marker genes was used in the study and transformation efficiencies reached over 50 independent transformed SCC per gram of infected cells. Best results were obtained when cells were infected at the exponential phase. A high density plating (500 mg/dish) gave significantly better results. As selective agent, kanamycin was inefficient for the selection of Monastrell transformed SCC since wild type cells were almost insensitive to this antibiotic whereas application of paromomycin resulted in very effective selection. Selected eyfp-expressing microcalli were grown until enough tissue was available to scale up a new transgenic SCC. These transgenic SCC lines were evaluated molecularly and phenotypically demonstrating the presence and integration of both transgenes, the absence of Agrobacterium contamination and the ability of the transformed SCC to grow in highly selective liquid medium. The methodology described here opens the possibility of improving the production of valuable metabolites. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:725-734, 2016. PMID:26871543</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4566059','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4566059"><span id="translatedtitle">Impacts of strigolactone on shoot branching under phosphate starvation in chrysanthemum (Dendranthema grandiflorum <span class="hlt">cv</span>. Jinba)</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Xi, Lin; Wen, Chao; Fang, Shuang; Chen, Xiaoli; Nie, Jing; Chu, JinFang; Yuan, Cunquan; Yan, Cunyu; Ma, Nan; Zhao, Liangjun</p> <p>2015-01-01</p> <p>Chrysanthemum (Dendranthema grandiflorum <span class="hlt">cv</span>. Jinba) shoot branching is determined by bud outgrowth during the vegetative growth stage. The degree of axillary bud outgrowth is highly influenced by environmental conditions, such as nutrient availability. Here, we demonstrated that phosphorus (Pi) starvation significantly reduces axillary bud outgrowth in chrysanthemum. A strigolactone (SL) biosynthesis gene, DgCCD7, was isolated and characterized as an ortholog of MAX3/DAD3/RMS5/D17. By using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS), three putative SLs were identified and levels of all three SLs showed strong increase under Pi starvation conditions. Determinations of the distribution of SLs and regulation of DgCCD7/8 in response to Pi changes in root indicate that SL acts systemically. However, temporal expression patterns of biosynthesis and signaling genes in nodes revealed that Pi starvation causes a local response of SL pathway. Treatment of node segments with or without auxin and Pi revealed that in the absence of exogenous auxin, Pi delayed axillary buds outgrowth and up-regulated local SL pathway genes. These data indicated that an auxin-SL regulatory loop responded to Pi starvation for delaying bud outgrowth locally, root biosynthesized SLs were transported acropetally and functioned in shoot branching inhibition under Pi starvation. We proposed that SLs contributed to chrysanthemum shoot branching control in response to Pi-limiting conditions in a systemic way. PMID:26442011</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/12552151','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/12552151"><span id="translatedtitle"><span class="hlt">Cv</span>ADH1, a member of short-chain alcohol dehydrogenase family, is inducible by gibberellin and sucrose in developing watermelon seeds.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Kim, Joonyul; Kang, Hong-Gyu; Jun, Sung-Hoon; Lee, Jinwon; Yim, Jieun; An, Gynheung</p> <p>2003-01-01</p> <p>To understand the molecular mechanisms that control seed formation, we selected a seed-preferential gene (<span class="hlt">Cv</span>ADH1) from the ESTs of developing watermelon seeds. RNA blot analysis and in situ localization showed that <span class="hlt">Cv</span>ADH1 was preferentially expressed in the nucellar tissue. The <span class="hlt">Cv</span>ADH1 protein shared about 50% homology with short-chain alcohol dehydrogenase including ABA2 in Arabidopsis thaliana, stem secoisolariciresinol dehydrogenase in Forsythia intermedia, and 3beta-hydroxysterol dehydrogenase in Digitalis lanata. We investigated gene-expression levels in seeds from both normally pollinated fruits and those made parthenocarpic via N-(2-chloro-4-pyridyl)-N'-phenylurea treatment, the latter of which lack zygotic tissues. Whereas the transcripts of <span class="hlt">Cv</span>ADH1 rapidly started to accumulate from about the pre-heart stage in normal seeds, they were not detectable in the parthenocarpic seeds. Treating the parthenogenic fruit with GA(3) strongly induced gene expression, up to the level accumulated in pollinated seeds. These results suggest that the <span class="hlt">Cv</span>ADH1 gene is induced in maternal tissues by signals made in the zygotic tissues, and that gibberellin might be one of those signals. We also observed that <span class="hlt">Cv</span>ADH1 expression was induced by sucrose in the parthenocarpic seeds. Therefore, we propose that the <span class="hlt">Cv</span>ADH1 gene is inducible by gibberellin, and that sucrose plays an important role in the maternal tissues of watermelon during early seed development. PMID:12552151</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=19840064486&hterms=physical+chemistry&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D70%26Ntt%3Dphysical%2Bchemistry','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=19840064486&hterms=physical+chemistry&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D70%26Ntt%3Dphysical%2Bchemistry"><span id="translatedtitle">The mineral chemistry and origin of inclusion matrix and meteorite matrix in the Allende <span class="hlt">CV</span>3 chondrite</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Kornacki, A. S.; Wood, J. A.</p> <p>1984-01-01</p> <p>The mineralogy and mineral chemistry of the inclusion and meteorite matrices in the Allende <span class="hlt">CV</span>3 chondrite are described, and the physical and chemical parameters of the conventional equilibrium condensation model of the origin of chondrite meteorites are evaluated. An alternative model of the origin of the mafic constituent of Allende inclusions is presented, on the basis of a new model of chondrule petrogenesis and the physical evolution of the primitive solar nebula. The model shows that the mineral chemistry of the olivine matrix in Allende <span class="hlt">CV</span>3 seems to preserve a good record of nebular and planetary processes, including: (1) vapor-to-solid condensation under relatively oxidizing nonequilibrium conditions; (2) Fe/Mg equilibration in the meteorite parent body; and (3) recrystallization and incipient melting in the solar nebula.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=20080012119&hterms=BDS&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D10%26Ntt%3DBDS','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=20080012119&hterms=BDS&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D10%26Ntt%3DBDS"><span id="translatedtitle">CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Terra-FM2_Edition1-<span class="hlt">CV</span>)</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Wielicki, Bruce A. (Principal Investigator)</p> <p></p> <p>Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-<span class="hlt">CV</span> CER_BDS_Aqua-FM4_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM1_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM2_Edition1-<span class="hlt">CV</span>. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=20080012118&hterms=BDS&qs=N%3D0%26Ntk%3DAll%26Ntx%3Dmode%2Bmatchall%26Ntt%3DBDS','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=20080012118&hterms=BDS&qs=N%3D0%26Ntk%3DAll%26Ntx%3Dmode%2Bmatchall%26Ntt%3DBDS"><span id="translatedtitle">CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Terra-FM1_Edition1-<span class="hlt">CV</span>)</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Wielicki, Bruce A. (Principal Investigator)</p> <p></p> <p>Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-<span class="hlt">CV</span> CER_BDS_Aqua-FM4_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM1_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM2_Edition1-<span class="hlt">CV</span>. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=20080012126&hterms=BDS&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D10%26Ntt%3DBDS','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=20080012126&hterms=BDS&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D10%26Ntt%3DBDS"><span id="translatedtitle">CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Aqua-FM3_Edition1-<span class="hlt">CV</span>)</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Wielicki, Bruce A. (Principal Investigator)</p> <p></p> <p>Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-<span class="hlt">CV</span> CER_BDS_Aqua-FM4_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM1_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM2_Edition1-<span class="hlt">CV</span>. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=20080012134&hterms=BDS&qs=N%3D0%26Ntk%3DAll%26Ntx%3Dmode%2Bmatchall%26Ntt%3DBDS','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=20080012134&hterms=BDS&qs=N%3D0%26Ntk%3DAll%26Ntx%3Dmode%2Bmatchall%26Ntt%3DBDS"><span id="translatedtitle">CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Aqua-FM4_Edition1-<span class="hlt">CV</span>)</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Wielicki, Bruce A. (Principal Investigator)</p> <p></p> <p>Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-<span class="hlt">CV</span> CER_BDS_Aqua-FM4_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM1_Edition1-<span class="hlt">CV</span> CER_BDS_Terra-FM2_Edition1-<span class="hlt">CV</span>. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2005-03-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/21441782','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/21441782"><span id="translatedtitle">Safety and immunogenicity of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-<span class="hlt">CV</span>) in Chilean children.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Lagos, Rosanna E; Muñoz, Alma E; Levine, Myron M; Lepetic, Alejandro; François, Nancy; Yarzabal, Juan Pablo; Schuerman, Lode</p> <p>2011-05-01</p> <p>The safety and immunogenicity of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-<span class="hlt">CV</span>, Synflorix™) were assessed in 240 healthy Chilean children randomized to receive 3 doses of PHiD-<span class="hlt">CV</span> (PHiD-<span class="hlt">CV</span> group) or hepatitis A vaccine (HAV control group) at 2-4-6 months of age. All were offered 1 HAV dose at 12 months (outside study). The PHiD-<span class="hlt">CV</span> group received a second HAV dose at 18-21 months and PHiD-<span class="hlt">CV</span> booster at 20-23 months. The HAV control group received 2 PHiD-<span class="hlt">CV</span> catch-up doses at 18-21 and 20-23 months. Adverse events were recorded and pneumococcal antibody responses and opsonophagocytic activity (OPA) were measured. Both PHiD-<span class="hlt">CV</span> vaccination schedules were well tolerated and immunogenic against the pneumococcal vaccine serotypes and protein D. The reactogenicity of PHiD-<span class="hlt">CV</span> primary, booster and catch-up doses was in line with previous PHiD-<span class="hlt">CV</span> studies, although generally higher than with HAV. For each vaccine serotype, the percentage of subjects with antibody concentrations ≥0.2 µg/ml (GSK's 22F-inhibition ELISA) was at least 93.2% following 3 PHiD-<span class="hlt">CV</span> primary doses and at least 97.4% post-booster; percentages with OPA titers ≥8 were at least 91.7% post-booster. After 2-dose catch-up, at least 94.3% of children had antibody concentrations ≥0.2 µg/ml against each serotype except 6B (84.3%); at least 95.2% had OPA titers ≥8 except against serotypes 1, 5 and 6B. In conclusion, the safety profiles of 2 PHiD-<span class="hlt">CV</span> vaccination schedules (3-dose primary plus booster and 2-dose catch-up) were in line with previous studies and PHiD-<span class="hlt">CV</span> was immunogenic for all 10 vaccine serotypes and protein D. PMID:21441782</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1080965','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1080965"><span id="translatedtitle">Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. <span class="hlt">cv</span> Reston 1</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Taylor, David C.; Weber, Nikolaus; Barton, Dennis L.; Underhill, Edward W.; Hogge, Lawrence R.; Weselake, Randall J.; Pomeroy, M. Keith</p> <p>1991-01-01</p> <p>Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. <span class="hlt">cv</span> Reston microspore-derived (MD) embryo culture system. TAGs accumulating during embryo development exhibited changes in acyl composition similar to those observed in developing zygotic embryos of the same <span class="hlt">cv</span>, particularly with respect to erucic and eicosenoic acids. However, MD embryos showed a much higher rate of incorporation of 14C-erucoyl moieties into TAGs in vitro than zygotic embryos. Homogenates of early-late cotyledonary stage MD embryos (14-29 days in culture) were assessed for the ability to incorporate 22:1 and 18:1 (oleoyl) moieties into glycerolipids. In the presence of [1-14C]22:1-coenzyme A (CoA) and various acyl acceptors, including glycerol-3-phosphate (G-3-P), radiolabeled erucoyl moieties were rapidly incorporated into the TAG fraction, but virtually excluded from other Kennedy Pathway intermediates as well as complex polar lipids. This pattern of erucoyl incorporation was unchanged during time course experiments or upon incubation of homogenates with chemicals known to inhibit Kennedy Pathway enzymes. In marked contrast, parallel experiments conducted using [1-14C]18:1-CoA and G-3-P indicated that 14C oleoyl moieties were incorporated into lyso-phosphatidic acids, phosphatidic acids, diacylglycerols, and TAGs of the Kennedy Pathway, as well as other complex polar lipids, such as phosphatidylcholines and phosphatidylethanolamines. When supplied with l-[2-3H(N)]G-3-P and [1-14C]22:1-CoA, the radiolabeled TAG pool contained both isotopes, indicating G-3-P to be a true acceptor of erucoyl moieties. Radio-high-performance liquid chromatography, argentation thin-layer chromatography/gas chromatography-mass spectrometry, and stereospecific analyses of radiolabeled TAGs indicated that 22:1 was selectively incorporated into the sn-3 position by a highly active diacylglycerol acyltransferase (DGAT; EC 2.3.1.20), while oleoyl moieties were</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4748781','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4748781"><span id="translatedtitle">Paraneoplastic Choreoathetosis in a Patient with Small Cell Lung Carcinoma and Anti-CRMP5/<span class="hlt">CV</span>2: A Case Report</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Aydin, Dogu; Somnier, Finn; Lassen, Lisbeth H.</p> <p>2016-01-01</p> <p>Introduction The occurrence of more or less monosymptomatic paraneoplastic choreoathetosis associated with anti-CRMP5/<span class="hlt">CV</span>2 antibodies is rare. Typically, such autoantibodies are associated with a more classical syndrome – paraneoplastic encephalomyelitis. Frequently, small cell lung carcinoma (SCLC) is the related neoplastic finding. Case Report We present a 71-year-old woman who developed visual symptoms with papilledema and chorea. Anti-CRMP5/<span class="hlt">CV</span>2 antibodies were a feature of both the serum and cerebrospinal fluid. Although SCLC was suspected already at the time of the initial examinations, no signs of primary or metastatic tumors were revealed on chest X-ray, MRI or whole-body PET scan. EEG and bronchoscopy were also unremarkable. However, 6 months after the onset, a repeated PET scan and subsequent bronchoscopic biopsy revealed SCLC. In spite of chemotherapy, the SCLC progressed, and the patient died 14 months after the onset of the symptoms. Conclusion We report paraneoplastic choreoathetosis associated with anti-CRMP5/<span class="hlt">CV</span>2 antibodies. Such published case histories are rare. Although expected, we did not find any reduced signal intensity at the basal ganglia on the T1-weighted or increased intensity on the T2-weighted MRI scans. PMID:26889151</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4475882','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4475882"><span id="translatedtitle">Effects of Colonization of the Roots of Domestic Rice (Oryza sativa L. <span class="hlt">cv</span>. Amaroo) by Burkholderia pseudomallei</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Constantinoiu, Constantin; Gardiner, Christopher; Warner, Jeffrey</p> <p>2015-01-01</p> <p>Burkholderia pseudomallei is a saprophytic bacterium that causes melioidosis and is often isolated from rice fields in Southeast Asia, where the infection incidence is high among rice field workers. The aim of this study was to investigate the relationship between this bacterium and rice through growth experiments where the effect of colonization of domestic rice (Oryza sativa L. <span class="hlt">cv</span> Amaroo) roots by B. pseudomallei could be observed. When B. pseudomallei was exposed to surface-sterilized seeds, the growth of both the root and the aerosphere was retarded compared to that in controls. The organism was found to localize in the root hairs and endodermis of the plant. A biofilm formed around the root and root structures that were colonized. Growth experiments with a wild rice species (Oryza meridionalis) produced similar retardation of growth, while another domestic cultivar (O. sativa L. <span class="hlt">cv</span> Koshihikari) did not show retarded growth. Here we report B. pseudomallei infection and inhibition of O. sativa L. <span class="hlt">cv</span> Amaroo, which might provide insights into plant interactions with this important human pathogen. PMID:25911477</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4552748','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4552748"><span id="translatedtitle">Psychometric Properties of the Obsessive-Compulsive Inventory-Child Version (OCI-<span class="hlt">CV</span>) in Chilean Children and Adolescents</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Martínez-González, Agustín E.; Rodríguez-Jiménez, Tíscar; Piqueras, José A.; Vera-Villarroel, Pablo; Godoy, Antonio</p> <p>2015-01-01</p> <p>In recent years, there has been a considerable increase in the development of assessment tools for obsessive-compulsive symptomatology in children and adolescents. The Obsessive Compulsive Inventory-Child Version (OCI-<span class="hlt">CV</span>) is a well-established assessment self-report, with special interest for the assessment of dimensions of Obsessive Compulsive Disorder (OCD). This instrument has shown to be useful for clinical and non-clinical populations in two languages (English and European Spanish). Thus, the aim of this study was to analyze the psychometric properties of the OCI-<span class="hlt">CV</span> in a Chilean community sample. The sample consisted of 816 children and adolescents with a mean age of 14.54 years (SD = 2.21; range = 10–18 years). Factor structure, internal consistency, test-retest reliability, convergent/divergent validity, and gender/age differences were examined. Confirmatory factor analysis showed a 6-factor structure (Doubting/Checking, Obsessing, Hoarding, Washing, Ordering, and Neutralizing) with one second-order factor. Good estimates of reliability (including internal consistency and test-retest), evidence supporting the validity, and small age and gender differences (higher levels of OCD symptomatology among older participants and women, respectively) are found. The OCI-<span class="hlt">CV</span> is also an adequate scale for the assessment of obsessions and compulsions in a general population of Chilean children and adolescents. PMID:26317404</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/19941088','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/19941088"><span id="translatedtitle">Biochemical properties of alpha-amylase from peel of Citrus sinensis <span class="hlt">cv</span>. Abosora.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Mohamed, Saleh Ahmed; Drees, Ehab A; El-Badry, Mohamed O; Fahmy, Afaf S</p> <p>2010-04-01</p> <p>alpha-Amylase activity was screened in the peel, as waste fruit, of 13 species and cultivars of Egyptian citrus. The species Citrus sinensis <span class="hlt">cv</span>. Abosora had the highest activity. alpha-Amylase AI from Abosora peel was purified to homogeneity using anion and cation-exchange, and gel filtration chromatographies. Molecular weight of alpha-amylase AI was found to be 42 kDa. The hydrolysis properties of alpha-amylase AI toward different substrates indicated that corn starch is the best substrate. The alpha-amylase had the highest activity toward glycogen compared with amylopectin and dextrin. Potato starch had low affinity toward alpha-amylase AI but it did not hydrolyze beta-cyclodextrin and dextran. Apparent Km for alpha-amylase AI was 5 mg (0.5%) starch/ml. alpha-Amylase AI showed optimum activity at pH 5.6 and 40 degrees C. The enzyme was thermally stable up to 40 degrees C and inactivated at 70 degrees C. The effect of mono and divalent metal ions were tested for the alpha-amylase AI. Ba2+ was found to have activating effect, where as Li+ had negligible effect on activity. The other metals caused inhibition effect. Activity of the alpha-amylase AI was increased one and half in the presence of 4 mM Ca2+ and was found to be partially inactivated at 10 mM Ca2+. The reduction of starch viscosity indicated that the enzyme is endoamylase. The results suggested that, in addition to citrus peel is a rich source of pectins and flavanoids, alpha-amylase AI from orange peel could be involved in the development and ripening of citrus fruit and may be used for juice processing. PMID:19941088</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2015AGUFMNH11A1889C&link_type=ABSTRACT','NASAADS'); return false;" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2015AGUFMNH11A1889C&link_type=ABSTRACT"><span id="translatedtitle">Scale-Dependent Measurements of Meteorite Strength and Fragmentation: Tamdakht (H5) and Allende (<span class="hlt">CV</span>3).</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Cotto-Figueroa, D.; Asphaug, E. I.; Garvie, L. A. J.; Morris, M. A.; Rai, A.; Chattopadhyay, A.; Johnston, J.; Borkowski, L.</p> <p>2015-12-01</p> <p>Meteorites are pieces of natural space debris, which have survived ejection from their parent bodies and passage through the Earth's atmosphere. As such, they provide a unique opportunity to study the fundamental physical and mechanical properties of early Solar System materials. But to date, few direct studies of physical properties have been conducted on meteoritic materials, in contrast to extensive chemical and isotopic analyses. It is important to determine these properties as they are related to disruption and fragmentation of bolides and asteroids, and activities related to sample return and hazardous asteroid mitigation. Here we present results from an ongoing suite of scale-dependent studies of meteorite strength and fragmentation. The meteorites studied are Tamdakht (H5), an ordinary chondrite that exhibits a heterogeneous structure criss-crossed with shock veins and centimeter-sized regions of white and light grey, and the carbonaceous chondrite Allende (<span class="hlt">CV</span>3), which suitable pieces are light grey with abundant chondrules and CAIs. Uniaxial compression tests are performed on meteorite cubes ranging from 0.5 to 4 centimeters using an Instron 5985 frame with a 250 kN load cell and compression fixtures with 145mm diameter radial platens. All tests are conducted at room temperature and in displacement control with a displacement rate of 0.25 mm per minute to ensure quasi-static conditions. A three-dimensional digital image correlation (DIC) system that enables noncontact measurement of displacement and strain fields is also used. Analysis of the strength and failure process of the two meteorite types is conducted and compared to terrestrial materials.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/25786733','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/25786733"><span id="translatedtitle">How will climate change influence grapevine <span class="hlt">cv</span>. Tempranillo photosynthesis under different soil textures?</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Leibar, Urtzi; Aizpurua, Ana; Unamunzaga, Olatz; Pascual, Inmaculada; Morales, Fermín</p> <p>2015-05-01</p> <p>While photosynthetic responses to elevated CO2, elevated temperature, or water availability have previously been reported for grapevine as responses to single stress factors, reports on the combined effect of multiple stress factors are scarce. In the present work, we evaluated effects of simulated climate change [CC; 700 ppm CO2, 28/18 °C, and 33/53% relative humidity (RH), day/night] versus current conditions (375 ppm CO2, 24/14 °C, and 45/65% RH), water availability (well-irrigated vs. water deficit), and different types of soil textures (41, 19, and 8% of soil clay contents) on grapevine (Vitis vinifera L. <span class="hlt">cv</span>. Tempranillo) photosynthesis. Plants were grown using the fruit-bearing cutting model. CC increased the photosynthetic activity of grapevine plants grown under well-watered conditions, but such beneficial effects of elevated CO2, elevated temperature, and low RH were abolished by water deficit. Under water-deficit conditions, plants subjected to CC conditions had similar photosynthetic rates as those grown under current conditions, despite their higher sub-stomatal CO2 concentrations. As expected, water deficit reduced photosynthetic activity in association with inducing stomatal closure that prevents water loss. Evidence for photosynthetic downregulation under elevated CO2 was observed, with decreases in photosynthetic capacity and leaf N content and increases in the C/N ratio in plants subjected to CC conditions. Soil texture had no marked effects on photosynthesis and did not modify the photosynthetic response to CC and water-deficit conditions. However, in mature well-irrigated plants grown in the soils with the highest sand content, an important decrease in stomatal conductance was observed as well as a slight decrease in the utilization of absorbed light in photosynthetic electron transport (measured as photochemical quenching), possibly related to a low water-retention capacity of these soils even under well-watered conditions. PMID:25786733</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/17357447','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/17357447"><span id="translatedtitle">Study on powdery mildew resistance transfer from S. cereale L.<span class="hlt">cv</span>. Weiling rye into wheat.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Zhang, Huai Yu; Ren, Zheng Long</p> <p>2007-02-01</p> <p>Weiling rye (S. cereale L.<span class="hlt">cv</span>.), a Chinese dwarf rye, confers high powdery mildew (Erysiphe gramininis f.sp.tritici) in China. My8443, a wheat cultivars infecting seriously powdery mildew disease, was used as the female parent and Weiling rye was used as the donor of powdery mildew resistance in the study. A new wheat-rye translocation line,named No.147,was developed from BC2F6 progenies of wheat cultivars My8443 and Weiling rye to transfer the resistance from Weiling rye to common wheat. The powdery mildew resistance of No.147 and its parents were investigated in seedling and adult stages by artificially inoculating the mixture of advanced pathogenic races in room and field and the single pathogenic race in room. Improved Giemsa C-banding technique and genomic in situ hybridization (GISH,Genomic in situ hybridization) were used to identify wheat and rye chromosomes. Acid polyacylamide gel electrophoresis(APAGE) separation of endosperm gliadin and simple sequence repeat(SSR) PCR amplification of 11 SCM-Secale cereale markers also were employed for 1RS confirmation in the study. The results showed that No.147 was a new 1BL/1RS wheat-rye chromosome translocation with high powdery mildew resistance derived from Weiling rye. The reason on the formation of the new wheat-rye chromosome translocation was analyzed. The utilizations of resistance gene resource derived from Chinese Weiling rye and the new 1BL/1RS translocation line in wheat genetics and breeding improvement were discussed in the paper. PMID:17357447</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=19900018265&hterms=natural+diamond&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D10%26Ntt%3Dnatural%2Bdiamond','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=19900018265&hterms=natural+diamond&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D10%26Ntt%3Dnatural%2Bdiamond"><span id="translatedtitle">Nature and origin of interstellar diamond from the Allende <span class="hlt">CV</span>3 meteorite</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Blake, David; Freund, Friedemann; Bunch, Ted; Krishnan, Kannan; Stampfer, Mitch; Chang, Sherwood; Tielens, Alexander G. G. M.</p> <p>1990-01-01</p> <p>Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende <span class="hlt">CV</span>3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on 3 mm EM grids. The AEM was performed on transmission-thin fragments of the material which overlay holes in the film, to eliminate interference from the substrate. Electron Spectroscopy for Chemical Analysis (ESCA) was performed on a large aliquot of C. Diamond was identified by selected area electron diffraction. Scanning Transmission Electron Microscope / Energy Dispersive X-ray (STEM-EDS) microanalyses of the C delta diamond, using a light-element detector, show that oxygen and possibly nitrogen are the only impurities consistently present. ESCA spectra from bulk C delta material confirm the presence of N at a level of 0.35 percent or less. Under UV irradiation a yellow-red fluorescence is observed, consistent with that of natural diamonds containing substitutional N. Electron Energy Loss Spectra (EELS) were recorded at 2 eV resolution from the C delta diamond, high pressure synthetic diamond, a diamond film produced in a low pressure plasma by chemical vapor deposition (CVD) on a heated silicon substrate (Roy, 1987), graphite, and amorphous arc sputtered carbon. Comparison of the carbon K edge shape and fine structure shows the Allende C delta phase to be largely diamond, but with a significant pre-edge absorption feature indicative of transitions of C 1s electrons into pi asterisk orbitals which are absent in the purely sp(3)-bonded diamond but present in graphite and amorphous carbon.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/22451246','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/22451246"><span id="translatedtitle">Effects and action mechanisms of Korean pear (Pyrus pyrifolia <span class="hlt">cv</span>. Shingo) on alcohol detoxification.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Lee, Ho-Sun; Isse, Toyoshi; Kawamoto, Toshihiro; Woo, Hyun-Su; Kim, An Keun; Park, Jong Y; Yang, Mihi</p> <p>2012-11-01</p> <p>Korean pear (Pyrus pyrifolia <span class="hlt">cv</span>. Shingo) has been used as a traditional medicine for alleviating alcohol hangover. However, scientific evidence for its effectiveness or mechanism is not clearly established. To investigate its mechanism of alcohol detoxification, both in vitro and in vivo studies were performed with an aldehyde dehydrogenase 2 (ALDH2) alternated animal model. The pear extract (10 mL/kg bw) was administered to Aldh2 normal (C57BL/6) and deficient (Aldh2 -/-) male mice. After 30 min, ethanol (1 g or 2 g/kg bw) was administered to the mice via gavage. Levels of alcohol and acetaldehyde in blood were quantified by GC/MS. First, it was observed that the pears stimulated both alcohol dehydrogenase (ADH) and ALDH activities by 2∼3-  and 1.3-fold in in vitro studies, respectively. Second, mouse PK data (AUC(∞) and C(max) ) showed that the pear extract decreased the alcohol level in blood regardless of ALDH2 genotype. Third, the pear increased the acetaldehyde level in blood in Aldh2 deficient mice but not in Aldh2 normal mice. Therefore, the consistent in vitro and in vivo data suggest that Korean pears stimulate the two key alcohol-metabolizing enzymes. These stimulations could be the main mechanism of the Korean pear for alcohol detoxification. Finally, the results suggest that polymorphisms of human ALDH2 could bring out individual variations in the effects of Korean pear on alcohol detoxification. PMID:22451246</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/2015DPS....4721320C','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/2015DPS....4721320C"><span id="translatedtitle">Scale-Dependent Measurements of Meteorite Strength and Fragmentation: Tamdakht (H5) and Allende (<span class="hlt">CV</span>3)</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Cotto-Figueroa, Desireé; Asphaug, Erik; Garvie, Laurence; Morris, Melissa; Rai, Ashwin; Chattopadhyay, Aditi; Chawla, Nikhilesh</p> <p>2015-11-01</p> <p>Meteorites are pieces of natural space debris, which have survived ejection from their parent bodies and passage through the Earth’s atmosphere. As such, they provide a unique opportunity to study the fundamental physical and mechanical properties of early Solar System materials. But to date, few direct studies of physical properties have been conducted on meteoritic materials, in contrast to extensive chemical and isotopic analyses. It is important to determine these properties as they are related to disruption and fragmentation of bolides and asteroids, and activities related to sample return and hazardous asteroid mitigation. Here we present results from an ongoing suite of scale-dependent studies of meteorite strength and fragmentation. The meteorites studied are Tamdakht (H5), an ordinary chondrite that exhibits a heterogeneous structure criss-crossed with shock veins and centimeter-sized regions of white and light grey, and the carbonaceous chondrite Allende (<span class="hlt">CV</span>3), which suitable pieces are light grey with abundant chondrules and CAIs. Uniaxial compression tests are performed on meteorite cubes ranging from 0.5 to 4 centimeters using an Instron 5985 frame with a 250 kN load cell and compression fixtures with 145mm diameter radial platens. All tests are conducted at room temperature and in displacement control with a displacement rate of 0.25 mm per minute to ensure quasi-static conditions. A three-dimensional digital image correlation (DIC) system that enables noncontact measurement of displacement and strain fields is also used. Analysis of the strength and failure process of the two meteorite types is conducted and compared to terrestrial materials.</p> </li> </ol> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li><a href="#" onclick='return showDiv("page_10");'>10</a></li> <li class="active"><span>11</span></li> <li><a href="#" onclick='return showDiv("page_12");'>12</a></li> <li><a href="#" onclick='return showDiv("page_13");'>13</a></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div><!-- col-sm-12 --> </div><!-- row --> </div><!-- page_11 --> <div id="page_12" class="hiddenDiv"> <div class="row"> <div class="col-sm-12"> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li><a href="#" onclick='return showDiv("page_10");'>10</a></li> <li><a href="#" onclick='return showDiv("page_11");'>11</a></li> <li class="active"><span>12</span></li> <li><a href="#" onclick='return showDiv("page_13");'>13</a></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div> </div> <div class="row"> <div class="col-sm-12"> <ol class="result-class" start="221"> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2012RaPC...81.1059C&link_type=ABSTRACT','NASAADS'); return false;" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2012RaPC...81.1059C&link_type=ABSTRACT"><span id="translatedtitle">Effect of quarantine treatments on the carbohydrate and organic acid content of mangoes (<span class="hlt">cv</span>. Tommy Atkins)</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Cruz, J. N.; Soares, C. A.; Fabbri, A. D. T.; Cordenunsi, B. R.; Sabato, S. F.</p> <p>2012-08-01</p> <p>Brazil is one of the largest mango producers and the third largest mango exporter worldwide. Irradiation treatment and its commercial feasibility have been studied in our country to make it possible to develop new markets and, consequently, to compete with the major exporters of mangoes, Mexico and India. This work was designed to compare irradiation treatment with the hot water dip treatment in mangoes <span class="hlt">cv</span>. Tommy Atkins for export and to verify that the main attributes for acceptance, color and texture, as well as carbohydrate and organic acid contents, were maintained. In this study, the fruit was divided into groups: control, hot water dip-treated (46 °C for 90 min), and irradiation-treated at doses of 0.4 kGy and 1.0 kGy. The fruit was stored at low temperature (11 °C±2) for 14 days and then at room temperature (23 °C±2) until the end of the study. The results indicated that the fruit given a dose of 1.0 kGy remained in a less advanced stage of ripening (stage 3) throughout the storage period, but experienced a greater loss of texture in the beginning of the experiment. It was noted that only the control group had higher levels of citric acid and succinic acid on the last day of the experiment. There were no significant differences in the total sugar content between any treatment groups. Gamma radiation can be used as a quarantine treatment and does not interfere negatively with the quality attributes of mangoes.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4223431','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4223431"><span id="translatedtitle">Prospective Study of Surgical Treatment of Acromegaly: Effects on Ghrelin, Weight, Adiposity, and Markers of <span class="hlt">CV</span> Risk</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Reyes-Vidal, Carlos; Fernandez, Jean Carlos; Bruce, Jeffrey N.; Crisman, Celina; Conwell, Irene M.; Kostadinov, Jane; Geer, Eliza B.; Post, Kalmon D.</p> <p>2014-01-01</p> <p>Context: Although epidemiological studies have found that GH and IGF-1 normalization reduce the excess mortality of active acromegaly to expected rates, cross-sectional data report some cardiovascular (<span class="hlt">CV</span>) risk markers to be less favorable in remission than active acromegaly. Objective: The objective of the study was to test the hypothesis that remission of acromegaly after surgical therapy increases weight and adiposity and some <span class="hlt">CV</span> risk markers and these changes are paralleled by a rise in ghrelin. Design: Forty-two adults with untreated, active acromegaly were studied prospectively. Changes in outcome measures from before to after surgery were assessed in 26 subjects achieving remission (normal IGF-1) and 16 with persistent active acromegaly (elevated IGF-1) after surgery. Setting: The study was conducted at tertiary referral centers for pituitary tumors. Main Outcome Measures: Endocrine, metabolic, and <span class="hlt">CV</span> risk parameters, anthropometrics, and body composition by dual-energy X-ray absorptiometry were measured. Results: Remission increased total ghrelin, body weight, waist circumference, C-reactive protein, homocysteine, high-density lipoprotein, and leptin and reduced systolic blood pressure, homeostasis model assessment score, triglycerides, and lipoprotein (a) by 6 months and for 32 ± 4 months after surgery. The ghrelin rise correlated with the fall in the levels of GH, IGF-1, and insulin and insulin resistance. Weight, waist circumference, and ghrelin did not increase significantly in the persistent active acromegaly group. Total body fat, trunk fat, and perentage total body fat increased by 1 year after surgery in 15 remission subjects: the increase in body fat correlated with the rise in total ghrelin. Conclusions: Although most markers of <span class="hlt">CV</span> risk improve with acromegaly remission after surgery, some markers and adiposity increase and are paralleled by a rise in total ghrelin, suggesting that these changes may be related. Understanding the mechanisms and</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/2013MPBu...40...12H','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/2013MPBu...40...12H"><span id="translatedtitle">Asteroid Lightcurve Analysis at Riverland Dingo Observatory (RDO): 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 <span class="hlt">CV</span> 26.</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Hills, Kevin</p> <p>2013-01-01</p> <p>Lightcurves for five asteroids selected from the Collaborative Asteroid Lightcurve Link (CALL) were obtained at Riverland Dingo Observatory (RDO) from 2012 July-September: 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 <span class="hlt">CV</span>26.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/26800776','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/26800776"><span id="translatedtitle">Construction of a subgenomic <span class="hlt">CV</span>-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Wehbe, Michel; Huguenin, Antoine; Leveque, Nicolas; Semler, Bert L; Hamze, Monzer; Andreoletti, Laurent; Bouin, Alexis</p> <p>2016-04-01</p> <p>Coxsackieviruses B (<span class="hlt">CV</span>-B) (Picornaviridae) are a common infectious cause of acute myocarditis in children and young adults, a disease, which is a precursor to 10-20% of chronic myocarditis and dilated cardiomyopathy (DCM) cases. The mechanisms involved in the disease progression from acute to chronic myocarditis phase and toward the DCM clinical stage are not fully understood but are influenced by both viral and host factors. Subgenomic replicons of <span class="hlt">CV</span>-B can be used to assess viral replication mechanisms in human cardiac cells and evaluate the effects of potential antiviral drugs on viral replication activities. Our objectives were to generate a reporter replicon from a cardiotropic prototype <span class="hlt">CV</span>-B3/28 strain and to characterize its replication properties into human cardiac primary cells. To obtain this replicon, a cDNA plasmid containing the full <span class="hlt">CV</span>-B3/28 genome flanked by a hammerhead ribozyme sequence and an MluI restriction site was generated and used as a platform for the insertion of sequences encoding emerald green fluorescent protein (EmGFP) in place of those encoding VP3. In vitro transcribed RNA from this plasmid was transfected into HeLa cells and human primary cardiac cells and was able to produce EmGFP and VP1-containing polypeptides. Moreover, non-structural protein biological activity was assessed by the specific cleavage of eIF4G1 by viral 2A(pro). Viral RNA replication was indirectly demonstrated by inhibition assays, fluoxetine was added to cell culture and prevented the EmGFP synthesis. Our results indicated that the EmGFP <span class="hlt">CV</span>-B3 replicon was able to replicate and translate as well as the <span class="hlt">CV</span>-B3/28 prototype strain. Our EmGFP <span class="hlt">CV</span>-B3 replicon will be a valuable tool to readily investigate <span class="hlt">CV</span>-B3 replication activities in human target cell models. PMID:26800776</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2329842','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2329842"><span id="translatedtitle">Preservation of Muscle Force in Mdx3<span class="hlt">cv</span> Mice Correlates with Low-Level Expression of a Near Full-Length Dystrophin Protein</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Li, Dejia; Yue, Yongping; Duan, Dongsheng</p> <p>2008-01-01</p> <p>The complete absence of dystrophin causes Duchenne muscular dystrophy. Its restoration by greater than 20% is needed to reduce muscle pathology and improve muscle force. Dystrophin levels lower than 20% are considered therapeutically irrelevant but are associated with a less severe phenotype in certain Becker muscular dystrophy patients. To understand the role of low-level dystrophin expression, we compared muscle force and pathology in mdx3<span class="hlt">cv</span> and mdx4<span class="hlt">cv</span> mice. Dystrophin was eliminated in mdx4<span class="hlt">cv</span> mouse muscle but was expressed in mdx3<span class="hlt">cv</span> mice as a near full-length protein at ∼5% of normal levels. Consistent with previous reports, we found dystrophic muscle pathology in both mouse strains. Surprisingly, mdx3<span class="hlt">cv</span> extensor digitorium longus muscle showed significantly higher tetanic force and was also more resistant to eccentric contraction-induced injury than mdx4<span class="hlt">cv</span> extensor digitorium longus muscle. Furthermore, mdx3<span class="hlt">cv</span> mice had stronger forelimb grip strength than mdx4<span class="hlt">cv</span> mice. Immunostaining revealed utrophin up-regulation in both mouse strains. The dystrophin-associated glycoprotein complex was also restored in the sarcolemma in both strains although at levels lower than those in normal mice. Our results suggest that subtherapeutic expression levels of near full-length, membrane-bound dystrophin, possibly in conjunction with up-regulated utrophin levels, may help maintain minimal muscle force but not arrest muscle degeneration or necrosis. Our findings provide valuable insight toward understanding delayed clinical onset and/or slow disease progression in certain Becker muscular dystrophy patients. PMID:18385524</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=20040140824&hterms=Inductively+Coupled+Plasma+Mass+Spectrometry&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D30%26Ntt%3DInductively%2BCoupled%2BPlasma%2BMass%2BSpectrometry','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=20040140824&hterms=Inductively+Coupled+Plasma+Mass+Spectrometry&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D30%26Ntt%3DInductively%2BCoupled%2BPlasma%2BMass%2BSpectrometry"><span id="translatedtitle">Mercury Abundances and Isotopic Compositions in the Murchison (CM) and Allende (<span class="hlt">CV</span>)Carbonaceous Chondrites</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Lauretta, D. S.; Klaue, B.; Blum, J. D.; Buseck, P. R.</p> <p>2001-01-01</p> <p>The abundance and isotopic composition of Hg was determined in bulk samples of both the Murchison (CM) and Allende (<span class="hlt">CV</span>) carbonaceous chondrites using single- and multi-collector inductively coupled plasma mass spectrometry (ICP-MS). The bulk abundances of Hg are 294 6 15 ng/g in Murchison and 30.0 6 1.5 ng/g in Allende. These values are within the range of previous measurements of bulk Hg abundances by neutron activation analysis (NAA). Prior studies suggested that both meteorites contain isotopically anomalous Hg, with d l 96/202Hg values for the anomalous, thermal-release components from bulk samples ranging from 2260 %o to 1440 9/00 in Murchison and from 2620 9/00 to 1540 9/00 in Allende (Jovanovic and Reed, 1976a; 1976b; Kumar and Goel, 1992). Our multi-collector ICP-MS measurements suggest that the relative abundances of all seven stable Hg isotopes in both meteorites are identical to terrestrial values within 0.2 to 0.5 9/00m. On-line thermal-release experiments were performed by coupling a programmable oven with the singlecollector ICP-MS. Powdered aliquots of each meteorite were linearly heated from room temperature to 900 C over twenty-five minutes under an Ar atmosphere to measure the isotopic composition of Hg released fiom the meteorites as a h c t i o n of temperature. In separate experiments, the release profiles of S and Se were determined simultaneously with Hg to constrain the Hg distribution within the meteorites and to evaluate the possibility of Se interferences in previous NAA studies. The Hg-release patterns differ between Allende and Murchison. The Hg-release profile for Allende contains two distinct peaks, at 225" and 343"C, whereas the profile for Murchison has only one peak, at 344 C. No isotopically anomalous Hg was detected in the thermal-release experiments at a precision level of 5 to 30 9/00, depending on the isotope ratio. In both meteorites the Hg peak at ;340"C correlates with a peak in the S-release profile. This correlation</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/2011AGUFMGP21B1000C','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/2011AGUFMGP21B1000C"><span id="translatedtitle">A secondary origin of chondrule magnetization in the Allende <span class="hlt">CV</span> carbonaceous chondrite</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Carporzen, L.; Fu, R.; Andrade Lima, E.; Weiss, B. P.</p> <p>2011-12-01</p> <p>Magnetic fields in the solar nebula may have played a key role in the radial transport of angular momentum and mass during the early accretional phase of the solar system. Chondrules and many calcium aluminum inclusions (CAIs), millimeter sized silicate objects found in most chondritic meteorites, were heated to high temperatures and cooled in the nebula and therefore may have recorded a thermoremanent magnetization (TRM) from the nebula field. Additionally, primitive magnetization in chondrules and CAIs may yield constraints about their mode of formation. However, any such primary magnetization may have been significantly altered during subsequent metamorphism and aqueous alteration on the parent asteroid. We performed two tests to determine the nebular origins of remanent magnetization in chondrules and refractory inclusions in the Allende <span class="hlt">CV</span>3 carbonaceous chondrite: 1) a classic paleomagnetic conglomerate test to identify post-accretional remagnetization events and 2) a unidirectionality test of subsamples taken from individual chondrules and CAIs. We conducted individual measurements of mutually oriented chondrules, CAIs, and matrix as well as SQUID microscope maps of the magnetic fields of 30 μm thin sections. All samples and thin sections were mutually oriented to within 5°. Our results confirm previous findings that all subsamples of the meteorite carry a unidirectional overprint blocked up to 260°-290°C (MT component). Chondrules and CAIs also carry a higher temperature (HT) remanence oriented in scattered directions unrelated to the direction of the MT overprint. We have confirmed that this HT magnetization is not an artifact of the demagnetization procedure but is a preterrestrial component. Measurements of subsamples of single chondrules and CAIs show that the HT magnetization is not unidirectional within each inclusion. Petrographic data suggests that most magnetic minerals in Allende were the product of parent body alteration. These facts suggest</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4732810','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4732810"><span id="translatedtitle">Transcriptomic Analysis of Grapevine (<span class="hlt">cv</span>. Summer Black) Leaf, Using the Illumina Platform</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Pervaiz, Tariq; Haifeng, Jia; Salman Haider, Muhammad; Cheng, Zhang; Cui, Mengjie; Wang, Mengqi; Cui, Liwen; Wang, Xicheng; Fang, Jinggui</p> <p>2016-01-01</p> <p>Proceeding to illumina sequencing, determining RNA integrity numbers for poly RNA were separated from each of the four developmental stages of <span class="hlt">cv</span>. Summer Black leaves by using Illumina HiSeq™ 2000. The sums of 272,941,656 reads were generated from vitis vinifera leaf at four different developmental stages, with more than 27 billion nucleotides of the sequence data. At each growth stage, RNA samples were indexed through unique nucleic acid identifiers and sequenced. KEGG annotation results depicted that the highest number of transcripts in 2,963 (2Avs4A) followed by 1Avs4A (2,920), and 3Avs4A (2,294) out of 15,614 (71%) transcripts were recorded. In comparison, a total of 1,532 transcripts were annotated in GOs, including Cellular component, with the highest number in “Cell part” 251 out of 353 transcripts (71.1%), followed by intracellular organelle 163 out of 353 transcripts (46.2%), while in molecular function and metabolic process 375 out of 525 (71.4%) transcripts, multicellular organism process 40 out of 525 (7.6%) transcripts in biological process were most common in 1Avs2A. While in case of 1Avs3A, cell part 476 out of 662 transcripts (71.9%), and membrane-bounded organelle 263 out of 662 transcripts (39.7%) were recorded in Cellular component. In the grapevine transcriptome, during the initial stages of leaf development 1Avs2A showed single transcript was down-regulated and none of them were up-regulated. While in comparison of 1A to 3A showed one up-regulated (photosystem II reaction center protein C) and one down regulated (conserved gene of unknown function) transcripts, during the hormone regulating pathway namely SAUR-like auxin-responsive protein family having 2 up-regulated and 7 down-regulated transcripts, phytochrome-associated protein showed 1 up-regulated and 9 down-regulated transcripts, whereas genes associated with the Leucine-rich repeat protein kinase family protein showed 7 up-regulated and 1 down-regulated transcript, meanwhile Auxin</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/18565697','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/18565697"><span id="translatedtitle">Protection levels in vaccinated heifers with experimental vaccines Brucella abortus M1-luc and <span class="hlt">INTA</span> 2.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Fiorentino, M A; Campos, E; Cravero, S; Arese, A; Paolicchi, F; Campero, C; Rossetti, O</p> <p>2008-12-10</p> <p>Brucella abortus M1-luc is a mutant strain derived from S19 vaccine strain in which most of bp26 sequence has been replaced by the luciferase coding gene. Strain I2 is a double mutant derived from M1-luc in which most of omp19 has been deleted without introduction of any genetic markers. In BALB/c mice, M1-luc presented equivalent performance to S19 regarding persistence, splenomegaly and protection against challenge. Interestingly, I2 was more attenuated than S19, with no reduction of protection against challenge. In order to evaluate the potential for vaccine use of these strains in the natural host, four groups of 15 heifers, 6-month old, were either non-vaccinated or vaccinated with S19, M1-luc or I2. To at reached 17-month old, heifers were synchronized with two doses of PGF2alpha and received natural service during 60 days with two bulls. Pregnant heifers were challenged at approximately six gestation months with virulent B. abortus S2308. Blood samples post-challenge of heifers were collected for serologic test as well as specimens of aborted fetuses and premature calves for bacterial isolation and histopathological analyses. Protection levels against abortion were 78.6% for S19, 81.8% for M1-luc and 45.5% for I2, compared to the 25% that did not abort from the non-vaccinated group. These results indicate that in bovines BP26 had no influence in protective capacity of S19, correlating with the results obtained in mice. However, contrarily to what was previously observed in mice, lack of expression of Omp19 rendered in less protection capacity of S19 in the natural host. PMID:18565697</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3462104','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3462104"><span id="translatedtitle">Growth promotion and colonization of switchgrass (Panicum virgatum) <span class="hlt">cv</span>. Alamo by bacterial endophyte Burkholderia phytofirmans strain PsJN</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p></p> <p>2012-01-01</p> <p>Background Switchgrass is one of the most promising bioenergy crop candidates for the US. It gives relatively high biomass yield and can grow on marginal lands. However, its yields vary from year to year and from location to location. Thus it is imperative to develop a low input and sustainable switchgrass feedstock production system. One of the most feasible ways to increase biomass yields is to harness benefits of microbial endophytes. Results We demonstrate that one of the most studied plant growth promoting bacterial endophytes, Burkholderia phytofirmans strain PsJN, is able to colonize and significantly promote growth of switchgrass <span class="hlt">cv</span>. Alamo under in vitro, growth chamber, and greenhouse conditions. In several in vitro experiments, the average fresh weight of PsJN-inoculated plants was approximately 50% higher than non-inoculated plants. When one-month-old seedlings were grown in a growth chamber for 30 days, the PsJN-inoculated Alamo plants had significantly higher shoot and root biomass compared to controls. Biomass yield (dry weight) averaged from five experiments was 54.1% higher in the inoculated treatment compared to non-inoculated control. Similar results were obtained in greenhouse experiments with transplants grown in 4-gallon pots for two months. The inoculated plants exhibited more early tillers and persistent growth vigor with 48.6% higher biomass than controls. We also found that PsJN could significantly promote growth of switchgrass <span class="hlt">cv</span>. Alamo under sub-optimal conditions. However, PsJN-mediated growth promotion in switchgrass is genotype specific. Conclusions Our results show B. phytofirmans strain PsJN significantly promotes growth of switchgrass <span class="hlt">cv</span>. Alamo under different conditions, especially in the early growth stages leading to enhanced production of tillers. This phenomenon may benefit switchgrass establishment in the first year. Moreover, PsJN significantly stimulated growth of switchgrass <span class="hlt">cv</span>. Alamo under sub-optimal conditions</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/26648117','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/26648117"><span id="translatedtitle">Maniwamycins: new quorum-sensing inhibitors against Chromobacterium violaceum <span class="hlt">CV</span>026 were isolated from Streptomyces sp. TOHO-M025.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Fukumoto, Atsushi; Murakami, Chikana; Anzai, Yojiro; Kato, Fumio</p> <p>2016-05-01</p> <p>Quorum sensing is an important microbial signaling system that controls the expression of many virulence genes. Maniwamycins C-F, new compounds and quorum-sensing inhibitors, were isolated from the culture broth of Streptomyces sp. TOHO-M025 using a silica gel column and preparative HPLC. The structures of maniwamycins were elucidated by spectroscopic analyses, including NMR. The compounds each have an azoxy moiety. All maniwamycins inhibited violacein synthesis, which is controlled by quorum sensing, in Chromobacterium violaceum <span class="hlt">CV</span>026. PMID:26648117</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=20040059293&hterms=dante&qs=N%3D0%26Ntk%3DAll%26Ntx%3Dmode%2Bmatchall%26Ntt%3Ddante','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=20040059293&hterms=dante&qs=N%3D0%26Ntk%3DAll%26Ntx%3Dmode%2Bmatchall%26Ntt%3Ddante"><span id="translatedtitle">Opaque Mineral Assemblages at Chondrule Boundaries in the Vigarano <span class="hlt">CV</span> Chondrite: Evidence for Gas-Solid Reactions Following Chondrule Formation</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Lauretta, Dante S.</p> <p>2004-01-01</p> <p>Recent studies of opaque minerals in primitive ordinary chondrites suggest that metal grains exposed at chondrule boundaries were corroded when volatile elements recondensed after the transient heating event responsible for chondrule formation. Metal grains at chondrule boundaries in the Bishunpur (LL3.1) chondrite are rimmed by troilite and fayalite. If these layers formed by gas solid reaction, then the composition of the corrosion products can provide information on the chondrule formation environment. Given the broad similarities among chondrules from different chondrite groups, similar scale layers should occur on chondrules in other primitive meteorite groups. Here I report on metal grains at chondrule boundaries in Vigarano (<span class="hlt">CV</span>3).</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/2011GeCoA..75.4752W','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/2011GeCoA..75.4752W"><span id="translatedtitle">Extremely Na- and Cl-rich chondrule from the <span class="hlt">CV</span>3 carbonaceous chondrite Allende</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Wasserburg, G. J.; Hutcheon, I. D.; Aléon, J.; Ramon, E. C.; Krot, A. N.; Nagashima, K.; Brearley, A. J.</p> <p>2011-09-01</p> <p>We report on a study of Al3509, a large Na- and Cl-rich, radially-zoned object from the oxidized <span class="hlt">CV</span> carbonaceous chondrite Allende. Al3509 consists of fine-grained ferroan olivine, ferroan Al-diopside, nepheline, sodalite, and andradite, and is crosscut by numerous veins of nepheline, sodalite, and ferroan Al-diopside. Some poorly-characterized phases of fine-grained material are also present; these phases contain no significant H 2O. The minerals listed above are commonly found in Allende CAIs and chondrules and are attributed to late-stage iron-alkali-halogen metasomatic alteration of primary high-temperature minerals. Textural observations indicate that Al3509 is an igneous object. However, no residual crystals that might be relicts of pre-existing CAI or chondrule minerals were identified. To establish the levels of 26Al and 36Cl originally present, 26Al- 26Mg and 36Cl- 36S isotopic systematics in sodalite were investigated. Al3509 shows no evidence of radiogenic 26Mg ∗, establishing an upper limit of the initial 26Al/ 27Al ratio of 3 × 10 -6. All sodalite grains measured show large but variable excesses of 36S, which, however, do not correlate with 35Cl/ 34S ratio. If these excesses are due to decay of 36Cl, local redistribution of radiogenic 36S ∗ after 36Cl had decayed is required. The oxygen-isotope pattern in Al3509 is the same as found in secondary minerals resulting from iron-alkali-halogen metasomatic alteration of Allende CAIs and chondrules and in melilite and anorthite of most CAIs in Allende. The oxygen-isotope data suggest that the secondary minerals precipitated from or equilibrated with a fluid of similar oxygen-isotope composition. These observations suggest that the formation of Al3509 and alteration products in CAIs and chondrules in Allende requires a very similar fluid phase, greatly enriched in volatiles (e.g., Na and Cl) and with Δ 17O ˜ -3‰. We infer that internal heating of planetesimals by 26Al would efficiently transfer</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/24234763','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/24234763"><span id="translatedtitle">Potential for phytoextraction of copper by Sinapis alba and Festuca rubra <span class="hlt">cv</span>. Merlin grown hydroponically and in vineyard soils.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Malagoli, Mario; Rossignolo, Virginia; Salvalaggio, Nico; Schiavon, Michela</p> <p>2014-03-01</p> <p>The extensive use of copper-bearing fungicides in vineyards is responsible for the accumulation of copper (Cu) in soils. Grass species able to accumulate Cu could be cultivated in the vineyard inter-rows for copper phytoextraction. In this study, the capacity of Festuca rubra <span class="hlt">cv</span> Merlin and Sinapis alba to tolerate and accumulate copper (Cu) was first investigated in a hydroponic system without the interference of soil chemical-physical properties. After the amendment of Cu (5 or 10 mg Cu l-(1)) to nutrient solution, shoot Cu concentration in F. rubra increased up to 108.63 mg Cu kg(-1) DW, more than three times higher than in S. alba (31.56 mg Cu kg(-1) DW). The relationship between Cu concentration in plants and external Cu was dose-dependent and species specific. Results obtained from the hydroponic experiment were confirmed by growing plants in pots containing soil collected from six Italian vineyards. The content of soil organic matter was crucial to enhance Cu tolerance and accumulation in the shoot tissues of both plant species. Although S. alba produced more biomass than F. rubra in most soils, F. rubra accumulated significantly more Cu (up to threefold to fourfold) in the shoots. Given these results, we recommended that F. rubra <span class="hlt">cv</span> Merlin could be cultivated in the vineyard rows to reduce excess Cu in vineyard soils. PMID:24234763</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1169040','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1169040"><span id="translatedtitle">Anaerobic n-Alkane Metabolism by a Sulfate-Reducing Bacterium, Desulfatibacillum aliphaticivorans Strain <span class="hlt">CV</span>2803T</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Cravo-Laureau, Cristiana; Grossi, Vincent; Raphel, Danielle; Matheron, Robert; Hirschler-Réa, Agnès</p> <p>2005-01-01</p> <p>The alkane-degrading, sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain <span class="hlt">CV</span>2803T, recently isolated from marine sediments, was investigated for n-alkane metabolism. The total cellular fatty acids of this strain had predominantly odd numbers of carbon atoms (C odd) when the strain was grown on a C-odd alkane (pentadecane) and even numbers of carbon atoms (C even) when it was grown on a C-even alkane (hexadecane). Detailed analyses of those fatty acids by gas chromatography/mass spectrometry allowed us to identify saturated 2-, 4-, 6-, and 8-methyl- and monounsaturated 6-methyl-branched fatty acids, with chain lengths that specifically correlated with those of the alkane. Growth of D. aliphaticivorans on perdeuterated hexadecane demonstrated that those methyl-branched fatty acids were directly derived from the substrate. In addition, cultures on pentadecane and hexadecane produced (1-methyltetradecyl)succinate and (1-methylpentadecyl)succinate, respectively. These results indicate that D. aliphaticivorans strain <span class="hlt">CV</span>2803T oxidizes n-alkanes into fatty acids anaerobically, via the addition of fumarate at C-2. Based on our observations and on literature data, a pathway for anaerobic n-alkane metabolism by D. aliphaticivorans is proposed. This involves the transformation of the initial alkylsuccinate into a 4-methyl-branched fatty acid which, in addition to catabolic reactions, can alternatively undergo chain elongation and desaturation to form storage fatty acids. PMID:16000749</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/23573031','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/23573031"><span id="translatedtitle">In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [ Cajanus cajan (L.) Millsp.] <span class="hlt">cv</span>. JKR105.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Krishna, Gaurav; Reddy, P Sairam; Ramteke, Pramod W; Rambabu, Pogiri; Sohrab, Sayed S; Rana, Debashis; Bhattacharya, Parthasarathi</p> <p>2011-10-01</p> <p>In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea <span class="hlt">cv</span>. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L(-1) 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L(-1) 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea <span class="hlt">cv</span>. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds. PMID:23573031</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/2014JASMS..25.1610A','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/2014JASMS..25.1610A"><span id="translatedtitle">Description of Gas-Phase Ion/Neutral Interactions in Differential Ion Mobility Spectrometry: <span class="hlt">CV</span> Prediction Using Calibration Runs</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Auerbach, David; Aspenleiter, Julia; Volmer, Dietrich A.</p> <p>2014-09-01</p> <p>Differential ion mobility spectrometry (DMS) coupled to mass spectrometry is increasingly used in both quantitative analyses of biological samples and as a means of removing background interferences for enhanced selectivity and improved quality of mass spectra. However, DMS separation efficiency using dry inert gases often lacks the required selectivity to achieve baseline separation. Polar gas-phase modifiers such as alcohols are therefore frequently employed to improve selectivity via clustering/declustering processes. The choice of an optimal modifier currently relies on trial and error experiments, making method development a tedious activity. It was the goal of this study to establish a means of <span class="hlt">CV</span> prediction for compounds using a homologous series of alcohols as gas-phase modifiers. This prediction was based on linear regression of compensation voltages of two calibration runs for the alcohols with the lowest and the highest molecular weights and readily available descriptors such as proton affinity and gas phase acidity of the modifier molecules. All experiments were performed on a commercial quadrupole linear ion trap mass spectrometer equipped with a DMS device between electrospray ionization source and entrance quadrupole lens. We evaluated our approach using a homologous series of 4-alkylbenzoic acids and a selection of 23 small molecules of high chemical diversity. Predicted <span class="hlt">CV</span> values typically deviated from the experimentally determined values by less than 0.5 V. Several test compounds changed their ion mobility behavior for the investigated gas phase modifiers (e.g., from type B to type A) and thus could thus not be evaluated.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/20927371','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/20927371"><span id="translatedtitle">Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4<span class="hlt">Cv</span>.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Pink, Ryan C; Eskiw, Christopher H; Caley, Daniel P; Carter, David R F</p> <p>2010-01-01</p> <p>Higher order chromatin folding is critical to a number of developmental processes, including the regulation of gene expression. Recently developed biochemical techniques such as RNA TRAP and chromosome conformation capture (3C) have provided us with the tools to probe chromosomal structures. These techniques have been applied to the β-globin locus, revealing a complex pattern of interactions with regions along the chromosome that the gene resides on. However, biochemical and microscopy data on the nature of β-globin interactions with other chromosomes is contradictory. Therefore we developed a novel 4C variant, Complete-genome 3C by vectorette amplification (4<span class="hlt">Cv</span>), which allows an unbiased and quantitative method to examine chromosomal structure. We have used 4<span class="hlt">Cv</span> to study the microenvironment of the β-globin locus in mice and show that a significant proportion of the interactions of β-globin are inter-chromosomal. Furthermore, our data show that in the liver, where the gene is active, β-globin is more likely to interact with other chromosomes, compared to the brain where the gene is silent and is more likely to interact with other regions along the same chromosome. Our data suggest that transcriptional activation of the β-globin locus leads to a change in nuclear position relative to the chromosome territory. PMID:20927371</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2013SPIE.8764E..0YG&link_type=ABSTRACT','NASAADS'); return false;" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2013SPIE.8764E..0YG&link_type=ABSTRACT"><span id="translatedtitle">Processor core for real time background identification of HD video based on Open<span class="hlt">CV</span> Gaussian mixture model algorithm</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Genovese, Mariangela; Napoli, Ettore</p> <p>2013-05-01</p> <p>The identification of moving objects is a fundamental step in computer vision processing chains. The development of low cost and lightweight smart cameras steadily increases the request of efficient and high performance circuits able to process high definition video in real time. The paper proposes two processor cores aimed to perform the real time background identification on High Definition (HD, 1920 1080 pixel) video streams. The implemented algorithm is the Open<span class="hlt">CV</span> version of the Gaussian Mixture Model (GMM), an high performance probabilistic algorithm for the segmentation of the background that is however computationally intensive and impossible to implement on general purpose CPU with the constraint of real time processing. In the proposed paper, the equations of the Open<span class="hlt">CV</span> GMM algorithm are optimized in such a way that a lightweight and low power implementation of the algorithm is obtained. The reported performances are also the result of the use of state of the art truncated binary multipliers and ROM compression techniques for the implementation of the non-linear functions. The first circuit has commercial FPGA devices as a target and provides speed and logic resource occupation that overcome previously proposed implementations. The second circuit is oriented to an ASIC (UMC-90nm) standard cell implementation. Both implementations are able to process more than 60 frames per second in 1080p format, a frame rate compatible with HD television.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/11539152','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/11539152"><span id="translatedtitle">Aqueous alteration of the Bali <span class="hlt">CV</span>3 chondrite: evidence from mineralogy, mineral chemistry, and oxygen isotopic compositions.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Keller, L P; Thomas, K L; Clayton, R N; Mayeda, T K; DeHart, J M; McKay, D S</p> <p>1994-12-01</p> <p>A petrographic, geochemical, and oxygen isotopic study of the Bali <span class="hlt">CV</span>3 carbonaceous chondrite revealed that the meteorite has undergone extensive deformation and aqueous alteration on its parent body. Deformation textures are common and include flattened chondrules, a well-developed foliation, and the presence of distinctive (100) planar defects in olivine. The occurrence of alteration products associated with the planar defects indicates that the deformation features formed prior to the episode of aqueous alteration. The secondary minerals produced during the alteration event include well-crystallized Mg-rich saponite, framboidal magnetite, and Ca-phosphates. The alteration products are not homogeneously distributed throughout the meteorite, but occur in regions adjacent to relatively unaltered material, such as veins of altered material following the foliation. The alteration assemblage formed under oxidizing conditions at relatively low temperatures (<100 degrees C). Altered regions in Bali have higher Na, Ca, and P contents than unaltered regions which suggests that the fluid phase carried significant dissolved solids. Oxygen isotopic compositions for unaltered regions in Bali fall within the field for other <span class="hlt">CV</span>3 whole-rocks, however, the oxygen isotopic compositions of the heavily altered material lie in the region for the CM and CR chondrites. The heavy-isotope enrichment of the altered regions in Bali suggest alteration conditions similar to those for the petrographic type-2 carbonaceous chondrites. PMID:11539152</p> </li> </ol> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li><a href="#" onclick='return showDiv("page_10");'>10</a></li> <li><a href="#" onclick='return showDiv("page_11");'>11</a></li> <li class="active"><span>12</span></li> <li><a href="#" onclick='return showDiv("page_13");'>13</a></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div><!-- col-sm-12 --> </div><!-- row --> </div><!-- page_12 --> <div id="page_13" class="hiddenDiv"> <div class="row"> <div class="col-sm-12"> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li><a href="#" onclick='return showDiv("page_10");'>10</a></li> <li><a href="#" onclick='return showDiv("page_11");'>11</a></li> <li><a href="#" onclick='return showDiv("page_12");'>12</a></li> <li class="active"><span>13</span></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div> </div> <div class="row"> <div class="col-sm-12"> <ol class="result-class" start="241"> <li> <p><a target="_blank" onclick="trackOutboundLink('http://hdl.handle.net/2060/19890018744','NASA-TRS'); return false;" href="http://hdl.handle.net/2060/19890018744"><span id="translatedtitle">Interpretation of F106B and <span class="hlt">CV</span>580 in-flight lightning data and form factor determination</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Rudolph, T.; Horembala, J.; Eriksen, F. J.; Weigel, H. S.; Elliott, J. R.; Parker, S. L.; Perala, R. A.</p> <p>1989-01-01</p> <p>Two topics of in-flight aircraft/lightning interaction are addressed. The first is the analysis of measured data from the NASA F106B Thunderstorm Research Aircraft and the <span class="hlt">CV</span>580 research program run by the FAA and Wright-Patterson Air Force Base. The <span class="hlt">CV</span>580 data was investigated in a mostly qualitative sense, while the F106B data was subjected to both statistical and quantitative analysis using linear triggered lightning finite difference models. The second main topic is the analysis of field mill data and the calibration of the field mill systems. The calibration of the F106B field mill system was investigated using an improved finite difference model of the aircraft having a spatial resolution of one-quarter meter. The calibration was applied to measured field mill data acquired during the 1985 thunderstorm season. The experimental determination of form factors useful for field mill calibration was also investigated both experimentally and analytically. The experimental effort involved the use of conducting scale models and an electrolytic tank. An analytic technique was developed to aid in the understanding of the experimental results.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2016M%26PS...51.1701D&link_type=ABSTRACT','NASAADS'); return false;" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2016M%26PS...51.1701D&link_type=ABSTRACT"><span id="translatedtitle">Magnetite in the unequilibrated CK chondrites: Implications for metamorphism and new insights into the relationship between the <span class="hlt">CV</span> and CK chondrites</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Dunn, Tasha L.; Gross, Juliane; Ivanova, Marina A.; Runyon, Simone E.; Bruck, Andrea M.</p> <p>2016-09-01</p> <p>Bulk isotopic and elemental compositions of <span class="hlt">CV</span> and CK chondrites have led to the suggestion that both originate from the same asteroid. It has been argued that magnetite compositions also support this model; however, magnetite has been studied almost exclusively in the equilibrated (type 4-6) CKs. Magnetite in seven unequilibrated CKs analyzed here is enriched in MgO, TiO2, and Al2O3 relative to the equilibrated CKs, suggesting that magnetite compositions are affected by metamorphism. Magnetite in CKs is compositionally distinct from CVs, particularly in abundances of Cr2O3, NiO, and TiO2. Although there are minor similarities between <span class="hlt">CV</span> and equilibrated CK chondrite magnetite, this is contrary to what we would expect if the CVs and CKs represent a single metamorphic sequence. <span class="hlt">CV</span> magnetite should resemble CK3 magnetite, as both were metamorphosed to type 3 conditions. Oxygen fugacities and temperatures of CVox and CK chondrites are also difficult to reconcile using existing <span class="hlt">CV</span>-CK parent body models. Mineral chemistries, which eliminate issues of bulk sample heterogeneity, provide a reliable alternative to techniques that involve a small amount of sample material. <span class="hlt">CV</span> and CK chondrite magnetite has distinct compositional differences that cannot be explained by metamorphism.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/2016M%26PS..tmp..354D','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/2016M%26PS..tmp..354D"><span id="translatedtitle">Magnetite in the unequilibrated CK chondrites: Implications for metamorphism and new insights into the relationship between the <span class="hlt">CV</span> and CK chondrites</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Dunn, Tasha L.; Gross, Juliane; Ivanova, Marina A.; Runyon, Simone E.; Bruck, Andrea M.</p> <p>2016-07-01</p> <p>Bulk isotopic and elemental compositions of <span class="hlt">CV</span> and CK chondrites have led to the suggestion that both originate from the same asteroid. It has been argued that magnetite compositions also support this model; however, magnetite has been studied almost exclusively in the equilibrated (type 4-6) CKs. Magnetite in seven unequilibrated CKs analyzed here is enriched in MgO, TiO2, and Al2O3 relative to the equilibrated CKs, suggesting that magnetite compositions are affected by metamorphism. Magnetite in CKs is compositionally distinct from CVs, particularly in abundances of Cr2O3, NiO, and TiO2. Although there are minor similarities between <span class="hlt">CV</span> and equilibrated CK chondrite magnetite, this is contrary to what we would expect if the CVs and CKs represent a single metamorphic sequence. <span class="hlt">CV</span> magnetite should resemble CK3 magnetite, as both were metamorphosed to type 3 conditions. Oxygen fugacities and temperatures of CVox and CK chondrites are also difficult to reconcile using existing <span class="hlt">CV</span>-CK parent body models. Mineral chemistries, which eliminate issues of bulk sample heterogeneity, provide a reliable alternative to techniques that involve a small amount of sample material. <span class="hlt">CV</span> and CK chondrite magnetite has distinct compositional differences that cannot be explained by metamorphism.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2015JAP...117o4101K&link_type=ABSTRACT','NASAADS'); return false;" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2015JAP...117o4101K&link_type=ABSTRACT"><span id="translatedtitle">Anomalous <span class="hlt">C-V</span> response correlated to relaxation processes in TiO2 thin film based-metal-insulator-metal capacitor: Effect of titanium and oxygen defects</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Kahouli, A.; Marichy, C.; Sylvestre, A.; Pinna, N.</p> <p>2015-04-01</p> <p>Capacitance-voltage (<span class="hlt">C-V</span>) and capacitance-frequency (C-f) measurements are performed on atomic layer deposited TiO2 thin films with top and bottom Au and Pt electrodes, respectively, over a large temperature and frequency range. A sharp capacitance peak/discontinuity (<span class="hlt">C-V</span> anomalous) is observed in the <span class="hlt">C-V</span> characteristics at various temperatures and voltages. It is demonstrated that this phenomenon is directly associated with oxygen vacancies. The <span class="hlt">C-V</span> peak irreversibility and dissymmetry at the reversal dc voltage are attributed to difference between the Schottky contacts at the metal/TiO2 interfaces. Dielectric analyses reveal two relaxation processes with degeneration of the activation energy. The low trap level of 0.60-0.65 eV is associated with the first ionized oxygen vacancy at low temperature, while the deep trap level of 1.05 eV is associated to the second ionized oxygen vacancy at high temperature. The DC conductivity of the films exhibits a transition temperature at 200 °C, suggesting a transition from a conduction regime governed by ionized oxygen vacancies to one governed by interstitial Ti3+ ions. Both the <span class="hlt">C-V</span> anomalous and relaxation processes in TiO2 arise from oxygen vacancies, while the conduction mechanism at high temperature is governed by interstitial titanium ions.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/1993Metic..28R.426R','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/1993Metic..28R.426R"><span id="translatedtitle">The Anatomy and Bulk Composition of CAI Rims in the Vigarano (<span class="hlt">CV</span>3) Chondrite</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Ruzicka, A.; Boynton, W. V.</p> <p>1993-07-01</p> <p>A striking feature of Ca,Al-rich inclusions (CAIs) in chondrites is the presence of mineralogical layers that typically form rim sequences up to 50 micrometers thick [1]. Many ideas regarding the origin of CAI rims have been proposed, but none are entirely satisfactory. The detailed mineralogy and bulk compositions of relatively unaltered CAI rims in the Vigarano (<span class="hlt">CV</span>3) chondrite described here provide constraints on hypotheses of rim formation. Rim Mineralogy: CAIs in Vigarano consist of melilite (mel)- and spinel (sp)- rich varieties, both of which are rimmed [2]. Around mel-rich objects, the layer sequence is CAI interior --> sp-rich layer (sometimes absent) --> mel/anorthite (anor) layer --> Ti-Al-rich clinopyroxene (Tpx) layer --> Al- diopside (Al-diop) layer --> olivine (ol) +/- Al-diop layer --> host matrix. The sequence around sp-rich objects differs from this in that the mel/anor layer is absent. Both the sp-rich layer around mel-cored CAIs and the cores of sp-rich CAIs in Vigarano are largely comprised of a fine-grained (<=1 micrometer) intergrowth of sp, Tpx, and minor mel and perovskite. These intergrowths are typically so fine grained that little internal texture is discernible. Mixing calculations suggest the presence of ~10 vol% Tpx in the sp-rich layer of two mel-cored CAIs, and the presence of ~35 vol% Tpx within one sp-cored CAI. The mel/anor layer is sometimes monomineralic, consisting of mel alone, or bimineralic, consisting of both mel and anor. Where bimineralic, anor typically occurs in the outer part of the layer. In places, anor (An(sub)99-100) has partially altered to nepheline and voids. Rim mel is systematically less gehlenitic than mel in the CAI interiors, especially compared to mel in the interior adjacent to the rims. The Tpx layer (>2 and up to 15 wt% TiO2) and Al-diop layer (<2 wt% TiO2) are monomineralic and show chemical zoning trends radial to the CAIs. Moving outward, TiO2 and Al2O3 generally decrease, while SiO2 and Mg</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/24152340','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/24152340"><span id="translatedtitle">Inhibition of hepatitis C virus by the cyanobacterial protein Microcystis viridis lectin: mechanistic differences between the high-mannose specific lectins MVL, <span class="hlt">CV</span>-N, and GNA.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Kachko, Alla; Loesgen, Sandra; Shahzad-Ul-Hussan, Syed; Tan, Wendy; Zubkova, Iryna; Takeda, Kazuyo; Wells, Frances; Rubin, Steven; Bewley, Carole A; Major, Marian E</p> <p>2013-12-01</p> <p>Plant or microbial lectins are known to exhibit potent antiviral activities against viruses with glycosylated surface proteins, yet the mechanism(s) by which these carbohydrate-binding proteins exert their antiviral activities is not fully understood. Hepatitis C virus (HCV) is known to possess glycosylated envelope proteins (gpE1E2) and to be potently inhibited by lectins. Here, we tested in detail the antiviral properties of the newly discovered Microcystis viridis lectin (MVL) along with cyanovirin-N (<span class="hlt">CV</span>-N) and Galanthus nivalis agglutinin (GNA) against cell culture HCV, as well as their binding properties toward viral particles, target cells, and recombinant HCV glycoproteins. Using infectivity assays, <span class="hlt">CV</span>-N, MVL, and GNA inhibited HCV with IC50 values of 0.6 nM, 30.4 nM, and 11.1 nM, respectively. Biolayer interferometry analysis demonstrated a higher affinity of GNA to immobilized recombinant HCV glycoproteins compared to <span class="hlt">CV</span>-N and MVL. Complementary studies, including fluorescence-activated cell sorting (FACS) analysis, confocal microscopy, and pre- and post-virus binding assays, showed a complex mechanism of inhibition for <span class="hlt">CV</span>-N and MVL that includes both viral and cell association, while GNA functions by binding directly to the viral particle. Combinations of GNA with <span class="hlt">CV</span>-N or MVL in HCV infection studies revealed synergistic inhibitory effects, which can be explained by different glycan recognition profiles of the mainly high-mannoside specific lectins, and supports the hypothesis that these lectins inhibit through different and complex modes of action. Our findings provide important insights into the mechanisms by which lectins inhibit HCV infection. Overall, the data suggest MVL and <span class="hlt">CV</span>-N have the potential for toxicity due to interactions with cellular proteins while GNA may be a better therapeutic agent due to specificity for the HCV gpE1E2. PMID:24152340</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4535146','PMC'); return false;" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4535146"><span id="translatedtitle">Identification and Expression of Two Novel Cytochrome P450 Genes, CYP6<span class="hlt">CV</span>1 and CYP9A38, in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pmc">PubMed Central</a></p> <p>Chen, Jun; Li, Chuan; Yang, Zhifan</p> <p>2015-01-01</p> <p>Cnaphalocrocis medinalis Güenée can cause severe losses in rice. Cytochrome P450s play crucial roles in the metabolism of allelochemicals in herbivorous insects. Two novel P450 cDNAs, CYP6<span class="hlt">CV</span>1 and CYP9A38, were cloned from the midgut of C. medinalis. CYP6<span class="hlt">CV</span>1 encodes a protein of 500 amino acid residues, while CYP9A38-predicted protein has 531 amino acid residues. Both cDNA-predicted proteins contain the conserved functional domains for all P450s. Phylogenetic analyses showed that CYP6<span class="hlt">CV</span>1 is grouped in the cluster containing CYP6B members, while CYP9A38 is in the cluster including CYP9 members. However, both clusters are contained in the same higher lineage. Homologous analysis revealed that CYP6<span class="hlt">CV</span>1 is most similar to CYP6B8, CYP6B7, CYP6B6, CYP6B2, and CYP6B4 with the highest amino acid identity of 41%. CYP9A38 is closest to CYP9A17, CYP9A21, CYP9A20, and CYP9A19 with the highest amino acid identity of 66%. Studies of temporal expression profiles revealed that CYP9A38 showed a steady increase in mRNA level during the five instar stages, but a low-expression level in pupae, and then presented at a high-expression level again in adults. Similar expression patterns were obtained with CYP6<span class="hlt">CV</span>1. In the fifth instar larvae, CYP6<span class="hlt">CV</span>1 was mainly expressed in midgut and fat bodies, whereas CYP9A38 was mainly expressed in midgut. Expression studies also revealed a 3.20-fold over-expression of CYP6<span class="hlt">CV</span>1 and 3.54-fold over-expression of CYP9A38 after larval exposure to host rice resistance. Our results suggest that both CYP6<span class="hlt">CV</span>1 and CYP9A38 may be involved in detoxification of rice phytochemicals. PMID:25896119</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://adsabs.harvard.edu/abs/1987GeCoA..51..329F','NASAADS'); return false;" href="http://adsabs.harvard.edu/abs/1987GeCoA..51..329F"><span id="translatedtitle">Al-26, Pu-244, Ti-50, REE, and trace element abundances in hibonite grains from CM and <span class="hlt">CV</span> meteorites</span></a></p> <p><a target="_blank" href="http://adsabs.harvard.edu/abstract_service.html">NASA Astrophysics Data System (ADS)</a></p> <p>Fahey, A. J.; Goswami, J. N.; McKeegan, K. D.; Zinner, E.</p> <p>1987-02-01</p> <p>Hibonites from the CM meteorites Murchison, Murray, and Cold Bokkeveld, and hibonites and Ti-rich pyroxene from the <span class="hlt">CV</span> chondrite Allende are studied. Electron microprobe measurements of major element concentrations and track and ion probe measurements of Mg and Ti isotopic ratios, rare earth elements (REEs), and trace element abundances are analyzed. Correlations between isotopic anomalies in Ti, Al-26, Pu-244, and Mg-26(asterisk) are examined. Ti isotopic anomalies are compared with REE and trace element abundance patterns. Reasons for the lack of Al-26 in the hibonites are investigated and discussed. It is observed that there is no correlation between the Ti isotopic compositions, and the presence of Mg-26(asterisk), Pu-244, and REE and trace element patterns in individual hibonite samples. The data reveal that hibonites are not interstellar dust grains but formed on a short time scale and in localized regions of the early solar system.</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://www.ncbi.nlm.nih.gov/pubmed/23398279','PUBMED'); return false;" href="http://www.ncbi.nlm.nih.gov/pubmed/23398279"><span id="translatedtitle">Effect of different production systems on chemical profiles of dwarf French bean (Phaseolus vulgaris L. <span class="hlt">cv</span>. Top Crop) pods.</span></a></p> <p><a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed">PubMed</a></p> <p>Jakopic, Jerneja; Slatnar, Ana; Mikulic-Petkovsek, Maja; Veberic, Robert; Stampar, Franci; Bavec, Franci; Bavec, Martina</p> <p>2013-03-13</p> <p>The chemical composition of dwarf French bean (Phaseolus vulgaris L.) <span class="hlt">cv</span>. Top Crop was compared among five production systems: conventional, integrated, organic, and biodynamic production systems and the control. Determination of sugars and organic acids was performed with a HPLC system, and identification of individual phenolic compounds using HPLC-MS. The chemical composition of the beans was unaffected by the production systems; however, the content levels of individual compounds were changed. The pods from integrated production contained the lowest levels of glucose and sucrose and the highest levels of catechin, procyanidin dimers, and a vanillic acid derivative. The control treatment, as well as organic and biodynamic productions, positively affected the levels of sugar content and caused a lower content of catechin and trans-p-coumaroylaldaric acids. Beans from the conventional production system contained the lowest levels of fructose, glucose, ascorbic acid, and many phenolics from various groups. PMID:23398279</p> </li> <li> <p><a target="_blank" onclick="trackOutboundLink('http://ntrs.nasa.gov/search.jsp?R=19870049284&hterms=trace+elements&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D90%26Ntt%3Dtrace%2Belements','NASA-TRS'); return false;" href="http://ntrs.nasa.gov/search.jsp?R=19870049284&hterms=trace+elements&qs=Ntx%3Dmode%2Bmatchall%26Ntk%3DAll%26N%3D0%26No%3D90%26Ntt%3Dtrace%2Belements"><span id="translatedtitle">Al-26, Pu-244, Ti-50, REE, and trace element abundances in hibonite grains from CM and <span class="hlt">CV</span> meteorites</span></a></p> <p><a target="_blank" href="http://ntrs.nasa.gov/search.jsp">NASA Technical Reports Server (NTRS)</a></p> <p>Fahey, A. J.; Mckeegan, K. D.; Zinner, E.; Goswami, J. N.</p> <p>1987-01-01</p> <p>Hibonites from the CM meteorites Murchison, Murray, and Cold Bokkeveld, and hibonites and Ti-rich pyroxene from the <span class="hlt">CV</span> chondrite Allende are studied. Electron microprobe measurements of major element concentrations and track and ion probe measurements of Mg and Ti isotopic ratios, rare earth elements (REEs), and trace element abundances are analyzed. Correlations between isotopic anomalies in Ti, Al-26, Pu-244, and Mg-26(asterisk) are examined. Ti isotopic anomalies are compared with REE and trace element abundance patterns. Reasons for the lack of Al-26 in the hibonites are investigated and discussed. It is observed that there is no correlation between the Ti isotopic compositions, and the presence of Mg-26(asterisk), Pu-244, and REE and trace element patterns in individual hibonite samples. The data reveal that hibonites are not interstellar dust grains but formed on a short time scale and in localized regions of the early solar system.</p> </li> </ol> <div class="pull-right"> <ul class="pagination"> <li><a href="#" onclick='return showDiv("page_1");'>«</a></li> <li><a href="#" onclick='return showDiv("page_9");'>9</a></li> <li><a href="#" onclick='return showDiv("page_10");'>10</a></li> <li><a href="#" onclick='return showDiv("page_11");'>11</a></li> <li><a href="#" onclick='return showDiv("page_12");'>12</a></li> <li class="active"><span>13</span></li> <li><a href="#" onclick='return showDiv("page_13");'>»</a></li> </ul> </div> </div><!-- col-sm-12 --> </div><!-- row --> </div><!-- page_13 --> <center> <div class="footer-extlink text-muted"><small>Some links on this page may take you to non-federal websites. 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