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INTA?Sat-1 First Earth Observation Mission  

Microsoft Academic Search

\\u000a This chapter is a description of the INTA?Sat-1 first Earth observation mission objectives and its development status. With\\u000a a mass around 100 kg and a 60 × 60 × 90 cm size compatible as an auxiliary payload for VEGA, Soyuz-ST and Dnepr, the project\\u000a is running now the Phase-C and will be ready for launch by the middle of 2011. This new

Manuel Angulo; Laura Seoane; Elisa Molina; Manuel Prieto; Oscar Rodriguez; Segundo Esteban; Jordi Palau; Stefania Cornara


CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains  

PubMed Central

CopraRNA (Comparative prediction algorithm for small RNA targets) is the most recent asset to the Freiburg RNA Tools webserver. It incorporates and extends the functionality of the existing tool IntaRNA (Interacting RNAs) in order to predict targets, interaction domains and consequently the regulatory networks of bacterial small RNA molecules. The CopraRNA prediction results are accompanied by extensive postprocessing methods such as functional enrichment analysis and visualization of interacting regions. Here, we introduce the functionality of the CopraRNA and IntaRNA webservers and give detailed explanations on their postprocessing functionalities. Both tools are freely accessible at PMID:24838564

Wright, Patrick R.; Georg, Jens; Mann, Martin; Sorescu, Dragos A.; Richter, Andreas S.; Lott, Steffen; Kleinkauf, Robert; Hess, Wolfgang R.; Backofen, Rolf



Characterization of IntA, a Bidirectional Site-Specific Recombinase Required for Conjugative Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42  

PubMed Central

Site-specific recombination occurs at short specific sequences, mediated by the cognate recombinases. IntA is a recombinase from Rhizobium etli CFN42 and belongs to the tyrosine recombinase family. It allows cointegration of plasmid p42a and the symbiotic plasmid via site-specific recombination between attachment regions (attA and attD) located in each replicon. Cointegration is needed for conjugative transfer of the symbiotic plasmid. To characterize this system, two plasmids harboring the corresponding attachment sites and intA were constructed. Introduction of these plasmids into R. etli revealed IntA-dependent recombination events occurring at high frequency. Interestingly, IntA promotes not only integration, but also excision events, albeit at a lower frequency. Thus, R. etli IntA appears to be a bidirectional recombinase. IntA was purified and used to set up electrophoretic mobility shift assays with linear fragments containing attA and attD. IntA-dependent retarded complexes were observed only with fragments containing either attA or attD. Specific retarded complexes, as well as normal in vivo recombination abilities, were seen even in derivatives harboring only a minimal attachment region (comprising the 5-bp central region flanked by 9- to 11-bp inverted repeats). DNase I-footprinting assays with IntA revealed specific protection of these zones. Mutations that disrupt the integrity of the 9- to 11-bp inverted repeats abolish both specific binding and recombination ability, while mutations in the 5-bp central region severely reduce both binding and recombination. These results show that IntA is a bidirectional recombinase that binds to att regions without requiring neighboring sequences as enhancers of recombination. PMID:23935046

Hernandez-Tamayo, Rogelio; Sohlenkamp, Christian; Puente, Jose Luis; Brom, Susana



The CV Period Minimum  

E-print Network

Using improved, up-to-date stellar input physics tested against observations of low-mass stars and brown dwarfs we calculate the secular evolution of low-mass donor CVs, including those which form with a brown dwarf donor star. Our models confirm the mismatch between the calculated minimum period (P_min ~ 70min) and the observed short-period cut-off (~80min) in the CV period histogram. Theoretical period distributions synthesized from our model sequences always show an accumulation of systems at the minimum period, a feature absent in the observed distribution. We suggest that non-magnetic CVs become unobservable as they are effectively trapped in permanent quiescence before they reach P_min, and that small-number statistics may hide the period spike for magnetic CVs. We calculate the minimum period for high mass transfer rate sequences and discuss the relevance of these for explaining the location of CV secondaries in the orbital period - spectral type diagram. We also show that a recently suggested revised mass-radius relation for low-mass main-sequence stars cannot explain the CV period gap.

Ulrich Kolb; Isabelle Baraffe



Bracken CV -1 Curriculum Vitae  

E-print Network

in a rocky intertidal system depend on invertebrate body size and algal cover. Ecology. #12;Bracken CV - 2 25. PLoS ONE 8: e62261. 22. Williams, S.L., M.E.S. Bracken, and E. Jones . 2013. Additive effects of physical stress and herbivores on intertidal seaweed diversity. Ecology 94: 1089-1101. 21. Bracken, M

Brody, James P.



E-print Network

Aug 14, 2014 ... B.S. computer science ... Mathematical Sciences Research Institute ... and analysis of early events in T-lymphocyte antigen-activated intracellular-signaling pathways, J. Comp. ... the effect of ion current modifiers, IEEE Trans.



Poston CV, Page 1 January 12, 2006  

E-print Network

Science Association President, North American Chinese Sociologists Association, 1995-1997 #12;Poston CV, Page 3 Past-President and Member of Council, North American Chinese Sociologists Association, 1997

Behmer, Spencer T.


The Leoville (CV3) accretionary breccia  

NASA Astrophysics Data System (ADS)

Leoville is a CV3 chondrite that containsn a large variety of inclusion, besides refractory incusions resembling those in Allende, fine-grained, dark inclusions are especially prominent. Some of these are xenoliths of material very similar to CM chondrites in texture, bulk composition, and oxygen isotopes. However, they show an unusually large range in the degree of hydrous alteration. Other dark inclusions are similar to host matrix. The CV3 host of this breccia is similar to other CV chondrites, although less metamorphosed than Allende. It is suggested that Leoville is a typical accretionary breccia whose parent body accreted after the Cm-like material represented by the xenoliths had formed and undergone alteration. After accretion Leoville suffered severe deformation, leading to foliation stronger than in any other chondrite, but the nature of the event that caused this remains unclear.

Kracher, A.; Keil, K.; Kallemeyn, G. W.; Wasson, J. T.; Clayton, R. N.; Huss, G. I.



CV (Mercado). February 2005 NORMAN MERCADO SILVA  

E-print Network

CV (Mercado). February 2005 NORMAN MERCADO SILVA Center for Limnology University of Wisconsin - Madison; 2001. PhD: Zoology and Limnology, Department of Zoology, University of Wisconsin ­ Madison, 2005 - Postdoctoral Associate. Center for Limnology (University of Wisconsin - Madison) 2004 - 05 Research Assistant

Mercado-Silva, Norman


Sabharwal -CV Page 1 Meghna Sabharwal  

E-print Network

Comparative Human Resources Practices High-Skilled Migration #12;May 2013 Sabharwal - CV Page 2 Teaching Scientists and Engineers from the United States to India Principal Investigator: Meghna Sabharwal Co of Wellness Programs in Local Governments Across The United States Investigators: Meghna Sabharwal and L

O'Toole, Alice J.


Earth, Moon, Sun, and CV Accretion Disks  

Microsoft Academic Search

Net tidal torque by the secondary on a misaligned accretion disk, like the\\u000anet tidal torque by the Moon and the Sun on the equatorial bulge of the\\u000aspinning and tilted Earth, is suggested by others to be a source to retrograde\\u000aprecession in non-magnetic, accreting Cataclysmic Variable (CV) Dwarf Novae\\u000asystems that show negative superhumps in their light curves.

M. M. Montgomery



MIZEX, 1984, NASA CV-990 flight report  

NASA Technical Reports Server (NTRS)

During June/July 1984, the NASA CV-990 Airborne Laboratory was utilized in a mission to overly the Fram Strait/East Greenland Sea marginal ice zone (MIZ) during the main summer marginal ice zone experiment (MIZEX '84). The eight data flights were coordinated where possible with overpasses of the Nimbus-7 satellite, and with measurement of sea ice, open ocean, and atmospheric properties at the surface. The surface research teams were based on seven research vessels, some with helicopters: (1) M/V Kvitbjorn, (2) M/V Polarqueen; (3) M/S Haakon Mosby; (4) a M/S H.U. Sverdrup, all from Norway; (5) F/S Polarstern from the Federal Republic of Germany; and (6) the USNS Lynch from the USA. There were also coordinated flights with the NRL P3, NOAA P3, Canadian CV580, and the French B-17 during the overlap portions of their respective missions. Analysis of the real-time data acquired during the mission and uncalibrated data stored on tape has served to indicate the mission was over 90% successful.



Stephen A. Mascaro S. MASCARO CV, PAGE 3  

E-print Network

Stephen A. Mascaro S. MASCARO ­ CV, PAGE 3 Journal Publications Pierce, M. and Mascaro, S. 2013-Star Search Algorithm," Proc. of the ASME Dynamic Systems and Control Conference (DSCC 2012), 8 pp. King, R: Expanding Wavefront and Simultaneous #12;Stephen A. Mascaro S. MASCARO ­ CV, PAGE 4 Operations," Proc

Mascaro, Stephen A.


C.V. Levine page 1 CURRICULUM VITAE  

E-print Network

C.V. Levine page 1 CURRICULUM VITAE Dr. Jonathan M. Levine Institute of Integrative Biology ETH Plant Ecology Graduate Seminar #12;C.V. Levine page 2 Current Topics in Ecology Model Formulation and Flora Hewlett Foundation Graduate Fellowship Research Grants- All but GeneMig awarded to J. M. Levine

Fischlin, Andreas


HPLC Analysis of Vitis vinifera cv. Cabernet Sauvignon and Vitis rotundifolia cv. Noble Wine Pigment Liquid Chromatographic Fractions  

Microsoft Academic Search

Vitis vinifera cv. Cabernet Sauvignon and Vitis rotundifolia cv. Noble red wines were made in three different skin contact time styles to investigate the anthocyanin content of polymers isolated from the wines by a low pressure column chromatographic technique. Acetaldehyde was added to one style wine after fermentation to further investigate its effect on the anthocyanin content of the polymers.

Tony V Johnston; Justin R. Morris


Razlike med sortama 'Merlot' (Vitis vinifera L. cv. 'Merlot') in 'Cabernet sauvignon' (Vitis vinifera L. cv. 'Cabernet sauvignon') ter primerjava kakovosti grozdja v Goriških brdih  

Microsoft Academic Search

DIFFERENCES BETWEEN CV.'MERLOT' (Vitis vinifera L. cv. 'Merlot') AND CV.'CABERNET SAUVIGNON' (Vitis vinifera L. cv. 'Cabernet sauvignon') AND COMPARISON OF GRAPE QUALITY IN GORIŠKA BRDA WINEGROWING DISTRICT Cv. 'Melot' and 'Cabarnet Sauvignon' are most widely spread red vine cultivars, grown on all continents. Both cultivars spread over the world simultaneously despite of their differences in amphelographic characteristics and agro-technical demands.



Micropropagation of sour cherry ( Prunus cerasus L.) cv. Šumadinka  

Microsoft Academic Search

Sour cherry cv. Šumadinka (Prunus cerasus L.) is the leading Yugoslav cultivar for production orchards. A method of micropropagation has been developed for the purpose of growing ‘Šumadinka’ on its own roots and for rapid multiplication.

Radosav Cerovi?; Djurdina Ruži?



van de Gevel -CV1 SASKIA L. VAN DE GEVEL  

E-print Network

of Costa Rica, Senior Seminar Capstone Course, Environmental Issues in Appalachia Course - Biogeography of Costa Rica, Environmental Issues in Appalachia, Introduction to the Natural 2005 Environment: Weather, Climate, and Biomes. #12;van de Gevel - CV2 Head

Thaxton, Christopher S.


Properties of NAC and CV[NAC] for Energy Models  

E-print Network

ESL-TR-93/05-03 Properties of CV [NAC] for Linear Energy Models David K. Ruch Sam Houston State University k Energy Systems Laboratory May 1993 1. Introduction and Notation The stability of the NAC estimate for the PRISM model has been shown em...- pirically to be greater than the stability of the other parameter estimates for the PRISM model [1]. The stability of the NAC parameter estimate, as measured by CV [NAC], is shown in this report to be superior to the slope and intercept estimates' stability...

Ruch, D. K.



REE Abundances in Matrix of Allende (CV) Chondrite  

NASA Astrophysics Data System (ADS)

In order to examine trace element distributions in matrix material of primitive chondrites, four interchondrule matrix specimens (sample weight ~100 micrograms) were carefully excavated using a microdrill from the petrographically characterized areas of the published sections of Allende (CV) chondrite and were precisely analyzed for REE, Ba, Sr, Rb, K, Ca and Mg by direct loading isotope dilution method (DL-IDMS).

Inoue, M.; Nakamura, N.; Kimura, M.



Computational simulation of CV combination preferences in babbling  

PubMed Central

There is a tendency for spoken consonant-vowel (CV) syllables, in babbling in particular, to show preferred combinations: labial consonants with central vowels, alveolars with front, and velars with back. This pattern was first described by MacNeilage and Davis, who found the evidence compatible with their “frame-then-content” (F/C) model. F/C postulates that CV syllables in babbling are produced with no control of the tongue (and therefore effectively random tongue positions) but systematic oscillation of the jaw. Articulatory Phonology (AP; Browman & Goldstein) predicts that CV preferences will depend on the degree of synergy of tongue movements for the C and V. We present computational modeling of both accounts using articulatory synthesis. Simulations found better correlations between patterns in babbling and the AP account than with the F/C model. These results indicate that the underlying assumptions of the F/C model are not supported and that the AP account provides a better and account with broader coverage by showing that articulatory synergies influence all CV syllables, not just the most common ones. PMID:24496111

Nam, Hosung; Goldstein, Louis M.; Giulivi, Sara; Levitt, Andrea G.; Whalen, D. H.



Boost Your CV! Training, Volunteering, Careers and Further Study  

E-print Network

: Building Your CV, Managing Projects, Application Forms, Effective Communication, Perfect Presentations to access an ILM Level 3 Award in Leadership, or the Community Volunteer Award. #12;Volunteering in Sport A wide range of opportunities to help you get involved in supporting Sport through Volunteering including

Evans, Paul


CV Marianne Espeland Marianne Espeland, PhD  

E-print Network

CV Marianne Espeland Marianne Espeland, PhD Museum of Comparative Zoology, Room 401c, Current position Harvard University. Museum of Comparative Zoology and Department of Organismic and Evolutionary biology. Postdoctoral fellow Education Oct. 2010 Doctor of Philosophy (PhD) in systematic zoology

Espeland, Marianne


Avaliação da cv. Cabernet Sauvignon para elaboração de vinho tinto  

Microsoft Academic Search

SUMMARY EVALUATION OF THE CV. CABERNET SAUVIGNON IN THE MANUFACTURE OF RED WINE. The Cabernet Sauvignon grape was developed in Bordeaux, France. It was introduced in Brazil in 1921, but its cultivation in the viticultural region of Serra Gaúcha only grew since 1980. It is used in the manufacture of aged or young red wines. Due to its importance, this

Luiz Antenor Rizzon; Alberto Miele



Hallas CV Page 1 of 8 Roger Hallas  

E-print Network

Bodies: AIDS, Bearing Witness, and the Queer Moving Image (Duke University Press, 2009). Reviews: Velvet by Flavia Monceri) #12;Hallas CV Page 2 of 8 Media International Australia, no. 143 (May 2012): 175 (review) M/C: Media & Culture (February 2011) (review by Danielle Mulholland) Gay & Lesbian Review (November

Segraves, Kari A.


CV Stockli 9/2012 CURRICULUM VITAE -Daniel F. Stockli  

E-print Network

Languages: English, German, French (fluent); Italian, Spanish, Arabic, Greek (elementary) #12;CV Stockli 9 and Institute for Geophysics Jackson School of Geosciences The University of Texas as Austin 1 University (International fission-track newsletter) 1995 - 1999 Teaching and research assistant at Stanford University 1994

Yang, Zong-Liang


Agrobacterium -mediated transformation of Solanum tuberosum L. cv. ‘Russet Burbank’  

Microsoft Academic Search

Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. ‘Russet Burbank’. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency of 60% in the absence of selection and 10% on medium containing 100

C. A. Newell; R. Rozman; M. A. Hinchee; E. C. Lawson; L. Haley; P. Sanders; W. Kaniewski; N. E. Tumer; R. B. Horsch; R. T. Fraley



HARVARDgazette04-17 FEBRUARY 2010 VOL. CV NO. 9  

E-print Network

arrives Feb. 5 with online coverage to come. ?p=36060 QUANTUM (NOT DIGITALHARVARDgazette04-17 FEBRUARY 2010 VOL. CV NO. 9 NEWS.HARVARD.EDU/GAZETTE Athletics for all Director of News and Media Relations Terry L. Murphy Managing Editor, Harvard Gazette Jim Concannon News


CV, T.S. Hopkins, 2009 Thomas Sawyer HOPKINS  

E-print Network

CV, T.S. Hopkins, 2009 1 Thomas Sawyer HOPKINS 288 Jefferson Rd, Princeton, NJ, USA Date of Birth: 12 February 1937 Place of Birth: Los Angeles, California Children: Faith 1967, Seth 1969 Married & Water Mass Analysis Eutrophication in Coastal Marine Systems Wind-Driven Circulation & Air

Parker, Matthew D. Brown


Indirect Moxibustion (CV4 and CV8) Ameliorates Chronic Fatigue: A Randomized, Double-Blind, Controlled Study  

PubMed Central

Abstract Objectives The antifatigue effect of indirect moxibustion and its antioxidant properties were investigated. Subjects and design A randomized, double-blind, controlled clinical trial was performed with 44 patients who had idiopathic chronic fatigue. The subjects were treated with a placebo or moxibustion (indirect moxibustion on CV4 and CV8 3 times per week for 4 weeks), and their fatigue severity was monitored using a self-rating numeric scale (NRS) and a visual analog scale (VAS). Serum level of reactive oxygen species and malondialdehyde (MDA), total antioxidant capacity, the activity of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase and total glutathione content, were determined before initial moxibustion therapy and after the 12th moxibustion treatment. Results The moxibustion group had a significantly lower fatigue severity score compared to the control for both the NRS (p<0.05) and VAS scores (p<0.01). The level of serum MDA was significantly lower in the moxibustion group than in the placebo group (p<0.05), whereas glutathione reductase activity and total glutathione content increased significantly following moxibustion (p<0.05). Conclusions The results provide clinical evidence for an antifatigue effect of indirect moxibustion at CV4 and CV8 and suggest that the effect is due to the antioxidant properties of moxibustion. PMID:22757691

Kim, Hyeong Geug; Yoo, Sa Ra; Park, Hye Jung



Memory immune response and safety of a booster dose of Japanese encephalitis chimeric virus vaccine (JE-CV) in JE-CV-primed children  

PubMed Central

Japanese encephalitis chimeric virus vaccine (JE-CV) is a licensed vaccine indicated in a single dose administration for primary immunization. This controlled phase III comparative trial enrolled children aged 36–42 mo in the Philippines. 345 children who had received one dose of JE-CV in a study two years earlier, received a JE-CV booster dose. 105 JE-vaccine-naïve children in general good health were randomized to receive JE-CV (JE-vaccine naïve group; 46 children) or varicella vaccine (safety control group; 59 children). JE neutralizing antibody titers were assessed using PRNT50. Immunological memory was observed in children who had received the primary dose of JE-CV before. Seven days after the JE-CV booster dose administration, 96.2% and 66.8% of children were seroprotected and had seroconverted, respectively, and the geometric mean titer (GMT) was 231 1/dil. Twenty-eight days after the JE-CV booster dose seroprotection and seroconversion were achieved in 100% and 95.3% of children, respectively, and the GMT was 2,242 1/dil. In contrast, only 15.4% of JE-CV-vaccine naïve children who had not received any prior JE vaccine were seroprotected seven days after they received JE-CV. One year after receiving the JE-CV booster dose, 99.4% of children remained seroprotected. We conclude that JE-CV is effective and safe, both as a single dose and when administrated as a booster dose. A booster dose increases the peak GMT above the peak level reached after primary immunization and the antibody persistence is maintained at least one year after the JE-CV booster dose administration. Five year follow up is ongoing. PMID:23442823

Feroldi, Emmanuel; Capeding, Maria Rosario; Boaz, Mark; Gailhardou, Sophia; Meric, Claude; Bouckenooghe, Alain



[Total Parenteral Nutrition and the Usefulness of CV Ports].  


Management of nutrition in cancer patients plays an important role in supporting anti-cancer treatment. Parenteral nutrition is considered to assist with nutrition in cancer patients. Central venous catheters(CVC)are useful for intravenous infusion of not only nutrients with high osmotic pressure but also chemotherapeutic drugs and other substances. Central venous access through CV ports reduces patient's burden and complications, and it contributes to maintaining a patient's quality of life(QOL). PMID:25335701

Washizawa, Naohiro; Yajima, Satoshi; Otsuka, Yuichiro; Koike, Junichi; Watanabe, Masashi; Kaneko, Hironori



Properties of a Cationic Peroxidase from Citrus jambhiri cv. Adalia  

Microsoft Academic Search

The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the\\u000a juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes\\u000a from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatography on diethylaminoethanol-cellulose,

Saleh A. Mohamed; Mohamed O. El-Badry; Ehab A. Drees; Afaf S. Fahmy



Circular Dichroism and Spectroscopic Studies of Vitis vinifera cv. Cabernet Sauvignon and Vitis rotundifolia cv. Noble Red Wine Liquid Chromatographic Fractions  

Microsoft Academic Search

2 * Abstract. Red wines were made with Vitis vinifera cv. Cabernet Sauvignon and Vitis rotundifolia cv. Noble grapes with three different skin contact times and with added acetaldehyde to investigate monoglucoside and diglucoside anthocyanin polymerization. The anthocyanins were extracted from the wines by a low-pressure liquid chromatographic (LC) procedure using a silica\\/PVPP column, producing three to four chromatographic fractions



Odour-active compounds in papaya fruit cv. Red Maradol.  


Application of solid-phase microextraction and simultaneous distillation-extraction combined with GC-FID, GC-MS, aroma extract dilution analysis, and odour activity value were used to analyse volatile compounds from papaya fruit cv. Red Maradol and to estimate the most odour-active compounds. The analyses led to the identification of 137 compounds; 118 of them were positively identified. Twenty-five odorants were considered as odour-active compounds and contribute to the typical papaya aroma, from which ethyl butanoate, benzyl isothiocyanate, 1-hexen-3-one, (E)-?-ionone, and methyl benzoate were the most odour-active compounds. PMID:24176322

Pino, Jorge A



No nebular magnetization in the Allende CV carbonaceous chondrite  

NASA Astrophysics Data System (ADS)

Magnetic fields in the solar nebula may have played a central role in mass and angular momentum transport in the protosolar disk and facilitated the accretion of the first planetesimals. Thought to be key evidence for this hypothesis is the high unblocking-temperature, randomly oriented magnetization in chondrules in the Allende CV carbonaceous chondrite. However, it has recently been realized that most of the ferromagnetic minerals in Allende are products of secondary processes on the parent planetesimal. Here we reevaluate the pre-accretional magnetism hypothesis for Allende using new paleomagnetic analyses of chondrules including the first measurements of mutually oriented subsamples from within individual chondrules. We confirm that Allende chondrules carry a high-temperature component of magnetization that is randomly oriented among chondrules. However, we find that subsamples of individual chondrules are also non-unidirectionally magnetized. Therefore, the high-temperature magnetization in Allende chondrules is not a record of nebular magnetic fields and is instead best explained by remagnetization during metasomatism in a <8 ?T magnetic field. This low field intensity suggests that any core dynamo on the CV parent body decayed before the end of metasomatism, likely <40 My after the formation of calcium aluminum-rich inclusions (CAIs). Despite widespread practice, the magnetization in Allende should not be used to constrain magnetic fields in the protosolar nebula.

Fu, R. R.; Lima, E. A.; Weiss, B. P.



Aqueous Alteration of the Grosnaja CV3 Carbonaceous Chondrite  

NASA Astrophysics Data System (ADS)

Previous petrographic studies have shown that aqueous alteration products are locally well developed in some of the CV3 falls [e.g., 1-3]. In this abstract, we describe our transmission electron microscope (TEM) study of the extent of aqueous alteration in matrix and in chondrules in the Grosnaja CV3 carbonaceous chondrite. Grosnaja is an observed fall and belongs to the oxidized subgroup of the CV chondrites [4]. We obtained fragments of Grosnaja from the Naturhistorisches Museum in Vienna. Regions of interest were extracted from polished thin sections and prepared for TEM observation by ion milling. Quantitative energy-dispersive X-ray (EDX) analyses were obtained using a JEOL 2000FX TEM equipped with a LINK thin- window EDX detector. Grosnaja has undergone aqueous alteration, which has resulted in the formation of phyllosilicates in matrix and in chondrules. The suprising result from Grosnaja is that three different types of phyllosilicates are intimately intergrown. Serpentine is the most abundant phyllosilicate in matrix and occurs as fine-grained packets along grain boundaries and as fracture-fillings and veinlets that cross cut olivine and pyroxene grains. Mixed with the serpentine are packets of fine-grained phyllosilicates with a distinct 1.4-nm basal spacing that is probably a chlorite group mineral. Rare packets of smectite occur as epitaxial intergrowths with olivine, but are not interstratified with serpentine as observed in the CI chondrites. Phyllosilicates in Grosnaja matrix occur with Mg-rich carbonates, fine-grained magnetite, chromite and pentlandite, and poorly-crystalline FeNi- oxide/hydroxides, which stain the matrix a brownish-red color. Some of the rust may be of terrestrial origin (Grosnaja fell in 1861). Although the matrix phyllosilicates are too small to obtain single-phase chemical analyses in the TEM, quantitative EDX analyses suggest that the serpentine contains significant Fe (Mg/Mg + Fe ~0.5). The serpentine/chlorite forms as an alteration product of matrix olivine. Olivine in matrix is equilibrated (Fa(sub)50). The matrix olivines contain numerous planar defects along (100) planes, which results in strong streaking along a* in electron diffraction patterns. These planar defects in matrix olivines are common in other CV chondrites, including Bali [3] and Mokoia [1]. Chondrule mesostasis is extensively altered to coarse-grained Na-saponite that is coherently interstratified with a 1.4-nm phyllosilicate (as shown by selected-area electron diffraction patterns). The 1.4-nm layers occur individually and in groups up to five layers wide. Serpentine has not been observed in chondrules. The Mg/Mg + Fe (atomic) ratio for the saponite is ~0.9, the same as for the host chondrule olivines. The formation of phyllosilicates in Grosnaja was controlled by local bulk compositions. The abundance of Na and Al in chondrule mesostasis stabilized Na-saponite, while in matrix, the high olivine content resulted in formation of serpentine. Grosnaja is unusual for a CV chondrite in that the dominant phyllosilicates in matrix are serpentine and chlorite, whereas smectite is the dominant phyllosilicate for the other altered CV chondrites [3]. This result suggests that alteration conditions were different for Grosnaja relative to the other CV falls. We believe that the occurrence of chlorite in both matrix and chondrules indicates alteration at temperatures higher than those experienced by the other altered CV chondrites. The heat source for the alteration reactions may be related to the thermal event that equilibrated matrix olivines. Acknowledgements: We thank G. Kurat of the Naturhistorisches Museum for samples of Grosnaja. This work was supported by NASA RTOPs 152-17-40-23 and 199-52-11-02. References: [1] Tomeoka K. and Buseck P. R. (1990) GCA, 54, 1745. [2] Keller L. P. and Buseck P. R. (1990) GCA, 54, 2113. [3] Keller L. P. and Thomas K. L. (1991) LPS XXII, 705. [4] McSween H. Y. (1977) GCA, 41, 1777.

Keller, L. P.; McKay, D. S.



INTA's Developments for UAS and Small Platforms: QUASAR  

Microsoft Academic Search

This paper will outline the objectives and mission, general features and roadmap and will give a brief description of the two main prototypes of QUASAR (Quicklook Unmanned Aerial SAR), the main project in Synthetic Aperture RADAR for small platforms carried out by the Spanish National Institute for Aerospace Technology. QUASAR is planned be released as a functional demonstrator in 2011

Juan Manuel Cuerda Muñoz; María José González Bonilla; Beatriz Gómez Miguel; Juan Ramón Larrañaga Sudupe; Marcos García Rodríguez



Cloning and sequencing of columbid circovirus (CoCV), a new circovirus from pigeons  

Microsoft Academic Search

Summary.  ?The complete nucleotide sequence of columbid circovirus (CoCV) isolated from pigeons is described. CoCV was amplified using\\u000a a consensus primer PCR approach directed against conserved sequences within the rep genes of vertebrate circoviruses. The genome of CoCV is circular and 2037 nt in size. It displays 55% homology to the genome\\u000a of psittacine beak and feather disease virus and is

A. Mankertz; K. Hattermann; B. Ehlers; D. Soike



CV-6209, a highly potent antagonist of platelet activating factor in vitro and in vivo.  


2-[N-acetyl-N-(2-methoxy-3-octadecylcarbamoyloxypropoxycarbonyl) aminomethyl]-1-ethylpyridinium chloride (CV-6209) inhibited aggregation of rabbit and human platelets induced by platelet activating factor (PAF) with the IC50 values of 7.5 X 10(-8) and 1.7 X 10(-7) M, respectively, and had little effects on the aggregation induced by arachidonic acid, ADP and collagen. The inhibitory effect of CV-6209 on the PAF-induced rabbit platelet aggregation was 104, 9, 8 and 3 times more potent than the PAF antagonists CV-3988, ONO-6240, Ginkgolide B and etizolam, respectively. CV-6209 inhibited [3H]serotonin release from rabbit platelets stimulated with PAF (3 X 10(-8) M) with a similar potency as the inhibition on the platelet aggregation. CV-6209 inhibited PAF (0.3 microgram/kg i.v.)-induced hypotension in rats (ED50, 0.009 mg/kg i.v.) with no effect on the hypotension induced by arachidonic acid, histamine, bradykinin and isoproterenol. CV-6209 (1 mg/kg) inhibited slightly the acetylcholine-induced hypotension. In rats, post-treatment with CV-6209 reversed the PAF (1 microgram/kg i.v.)-induced hypotension rapidly (ED50, 0.0046 mg/kg i.v.); CV-6209 was 74, 20, 185 and over 2100 times more potent than CV-3988, ONO-6240, Ginkgolide B and etizolam, respectively. Thus, the relative potency of the anti-PAF action of PAF analog (CV-6209, CV-3988 and ONO-6240) differed little between the inhibition of PAF-induced platelet aggregation and the reversal of PAF-induced hypotension, but that of nonPAF analogs (Ginkgolide B and etizolam) differed greatly with these assay systems, when standardized with CV-6209.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:3612533

Terashita, Z; Imura, Y; Takatani, M; Tsushima, S; Nishikawa, K



Collisional facilitation of aqueous alteration of CM and CV carbonaceous chondrites  

NASA Astrophysics Data System (ADS)

CM chondrites exhibit a strong correlation between the degree of alteration and the extent of particle alignment (i.e., the strength of the petrofabric). It seems likely that the S1 shock stage of essentially every CM and the high matrix abundance (˜70 vol.%) of these samples ensured that the shock waves that produced CM petrofabrics (by collapsing matrix pores and squeezing chondrules into pore spaces) were significantly attenuated and were too weak to damage olivine crystal lattices. Random collisions on the CM body produced petrofabrics and created fractures in the target rocks. Subsequent impact-mobilization of water caused hydrated phases to form preferentially in the more-fractured regions (those with the strongest petrofabrics); the less-deformed, less-fractured CM regions experienced lower degrees of aqueous alteration. Many CV3 chondrites also have petrofabrics: roughly half are from the oxidized Bali-like subgroup (CV3OxB), roughly half are from the reduced subgroup (CV3R) and none is from the oxidized Allende-like subgroup (CV3OxA) (which is less altered than CV3OxB). Nearly all CVs with petrofabrics are S3-S4 and nearly all CVs that lack petrofabrics are S1. Oxidized CVs have much higher porosities (typically 20-28%) than reduced CVs (0.6-8%), facilitating more-extensive aqueous alteration. The CV3R chondrites formed from low-porosity material that inhibited oxidation during alteration. The oxidized CV subgroups formed from higher-porosity materials. The CV3OxB samples were shocked, became extensively fractured and developed petrofabrics; the CV3OxA samples were not shocked and never developed petrofabrics. When water was mobilized, both sets of porous CV chondrites became oxidized; the more-fractured CV3OxB subgroup was more severely altered.

Rubin, Alan E.



Methyleugenol in Ocimum basilicum L. Cv. genovese gigante.  


Ocimum basilicum cv. Genovese Gigante is the basil cultivar used the most in the production of a typical Italian sauce called pesto. The aromatic composition of plants at different growth stages was determined. Plants from different areas of northwestern Italy were analyzed at 4 and 6 weeks after sowing and showed methyleugenol and eugenol as the main components. The content of these compounds was correlated with plant height rather than plant age. Particularly, methyleugenol was predominant in plants up to 10 cm in height, whereas eugenol was prevalent in taller plants. These results are important in the evaluation of risk to human health posed by dietary ingestion of methyleugenol contained in pesto. PMID:11170620

Miele, M; Dondero, R; Ciarallo, G; Mazzei, M



Refining the evaluation of bipolar II: beyond the strict SCID-CV guidelines for hypomania  

Microsoft Academic Search

Background: The prevalence of bipolar II disorder in depressed outpatients is much higher than previously reported, a finding probably related to systematic probing for past hypomania by trained clinicians. Our objective was to further refine the strict SCID-CV guidelines for hypomania in depressed outpatients. Methods: 168 consecutive outpatients presenting with major depression were systematically interviewed with the SCID-CV about all

Franco Benazzi; Hagop S. Akiskal



Martin O. Saar CV, Page 1 of 17 10/1/2009 CURRICULUM VITAE  

E-print Network

Martin O. Saar CV, Page 1 of 17 10/1/2009 CURRICULUM VITAE Martin O. Saar E-mail: saar George and Orpha Gibson Chair of Hydrogeology and Geofluids, University of Minnesota ­ Twin Cities Fellowship for Outstanding Scholastic Achievements, University of Oregon #12;Martin O. Saar CV, Page 2 of 17

Saar, Martin O.


Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) Somaclones  

NASA Technical Reports Server (NTRS)

The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.

Campbell, W. F.; Carman, J. G.; Hashim, Z. N.



Linear and nonlinear interpretation of CV-580 lightning data  

NASA Technical Reports Server (NTRS)

Numerical models developed for the study of lightning strike data acquired by in-flight aircraft are applied to the data measured on the CV-580. The basic technique used is the three dimensional time domain finite difference solution of Maxwell's equations. Both linear and nonlinear models are used in the analysis. In the linear model, the lightning channel and the aircraft are assumed to form a linear time invariant system. A transfer function technique can then be used to study the response of the aircraft to a given lightning strike current. Conversely, the lightning current can be inferred from the measured response. In the nonlinear model, the conductivity of air in the vicinity of the aircraft is calculated and incorporated into the solution of the Maxwell's equations. The nonlinear model thus simulates corona formation and air breakdown. Results obtained from the models are in reasonable agreement with the measured data. This study provides another validation of the models and increases confidence that the models may be used to predict aircraft response to any general lightning strike.

Ng, Poh H.; Rudolph, Terence H.; Perala, Rodney A.



Flipping the Switch from Monomeric to Dimeric CV-N Has Little Effect on Antiviral Activity  

Microsoft Academic Search

Cyanovirin-N can exist in solution in monomeric and domain-swapped dimeric forms, with HIV-antiviral activity being reported for both. Here we present results for CV-N variants that form stable solution dimers: the obligate dimer [?Q50]CV-N and the preferential dimer [S52P]CV-N. These variants exhibit comparable ?G values (10.6 ± 0.5 and 9.4 ± 0.5 kcal.mol?1, respectively), similar to that of stabilized, monomeric

Laura G. Barrientos; Fátima Lasala; Rafael Delgado; Anthony Sanchez; Angela M. Gronenborn



Candesartan (CV11974) dissociates slowly from the angiotensin AT 1 receptor  

Microsoft Academic Search

The mechanisms of the insurmountable antagonism of 2-ethoxy-1-[[2?-(1 H-tetrazol-5-yl)biphenyl-4-yl]methyl]-1 H-benzimidazole-7-carboxylic acid, candesartan (CV-11974), an angiotensin AT1 receptor antagonist, on angiotensin II-induced rabbit aortic contraction were examined in contraction and binding studies. Preincubation of the rabbit aorta with CV-11974 (0.1 nM) for 30 min reduced the maximal contractile response to angiotensin II by approximately 50%. This insurmountable antagonism of CV-11974 was

Mami Ojima; Yoshiyuki Inada; Yumiko Shibouta; Takeo Wada; Tsukasa Sanada; Keiji Kubo; Kohei Nishikawa



Evaluation of antitranspirants on creeping bentgrass (Agrostis palustris Huds., cv. 'Penncros') and bermudagrass (Cynodon dactylon (L.) Pers. X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway')  

E-print Network

on red fescue (Festuca rubra L. cv. S-59) (3). PMA decreased transpiration rates 20/ by reducing stomatal aperture, with- out reducing the shoot growth rate, but with an increase of 1. 6 C in leaf temperature. Applications of PMA at this concentration... on red fescue (Festuca rubra L. cv. S-59) (3). PMA decreased transpiration rates 20/ by reducing stomatal aperture, with- out reducing the shoot growth rate, but with an increase of 1. 6 C in leaf temperature. Applications of PMA at this concentration...

Stahnke, Gwen Kristine



Dr. Matas Alvarado (CV breve) Investigador Titular del Departamento de Computacin del CINVESTAV, Miembro Regular de  

E-print Network

Dr. Mat�as Alvarado (CV breve) Investigador Titular del Departamento de Computaci�n del CINVESTAV� Mat�as Alvarado Mentado Curriculum Vitae CURRENT POSITION Research Scientist (Investigador CINVESTAV 3

Alvarado, Matías


Limited geographic distribution of the novel cyclovirus CyCV-VN.  


A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600?CSF specimens from patients with suspected CNS infections in northern Vietnam, Cambodia, Nepal and The Netherlands were screened for the presence of CyCV-VN but all were negative. Sequence comparison and phylogenetic analysis between CyCV-VN and another novel cyclovirus recently identified in CSF from Malawian patients indicated that these represent distinct cycloviral species, albeit phylogenetically closely related. The data suggest that CyCV-VN has a limited geographic distribution within southern and central Vietnam. Further research is needed to determine the global distribution and diversity of cycloviruses and importantly their possible association with human disease. PMID:24495921

Le, Van Tan; de Jong, Menno D; Nguyen, Van Kinh; Nguyen, Vu Trung; Taylor, Walter; Wertheim, Heiman F L; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Nguyen, Hanh Uyen; Giri, Abhishek; Nguyen, Thi Thuy Chinh Bkrong; Ho, Dang Trung Nghia; Farrar, Jeremy; Bryant, Juliet E; Tran, Tinh Hien; Nguyen, Van Vinh Chau; van Doorn, H Rogier



Arbuscular mycorrhizal fungi enhance tolerance to bicarbonate in Rosa multiflora cv. burr  

E-print Network

from root free sand of two compartmental pot cultures of Trifolium subterraneum L. cv. Mount Baker (subterranean clover) (Joner and Johansen, 2000). Glomus intraradices has been shown to take up P from adenosine monophosphate ( 32 P-labelled AMP... from root free sand of two compartmental pot cultures of Trifolium subterraneum L. cv. Mount Baker (subterranean clover) (Joner and Johansen, 2000). Glomus intraradices has been shown to take up P from adenosine monophosphate ( 32 P-labelled AMP...

Cartmill, Andrew David



Full split C-V method for parameter extraction in ultra thin BOX FDSOI MOS devices  

NASA Astrophysics Data System (ADS)

The feasibility of full split C-V method in ultra-thin body and BOX (UTBB) FDSOI devices is demonstrated, emphasizing the usefulness of gate-to-bulk capacitance. The split C-V measurements carried out on both gate-to-channel and gate-to-bulk mode are shown to be consistent with TCAD simulation. This enabled us to propose an improved parameter extraction methodology for the whole vertical FDSOI stack from gate to substrate using back biasing effect.

Shin, Minju; Shi, Ming; Mouis, Mireille; Cros, Antoine; Josse, Emmanuel; Kim, Gyu-Tae; Ghibaudo, Gérard



Visualization of HDF/HDF-EOS Format Earth Observing System Data Using the ISIS "cv" Program  

NASA Astrophysics Data System (ADS)

The "cv" (Cube Visualization) program has been used for a number of years as part of the ISIS image processing system (Integrated Software for Imagers and Spectrometers) [1],[2]. In addition to reading the native ISIS image cube format, "cv" has recently been enhanced to directly read the HDF and HDF-EOS file formats used for distributing data from various Earth Observing System (EOS) Missions (e.g. the Terra and Landsat-7 satellites). Files based on HDF Version 4.x are supported; files based on the new HDF 5.x are not yet supported. "cv" is now packaged so that it can be used without installing the rest of the ISIS software system. The capabilities of the program include: Displays (as images) any Swath/Grid data fields in HDF-EOS files; Displays (as images) any Scientific Data Set (SDS) data fields in HDF files; Combines multiple HDF/HDF-EOS fields to form one display object; Subarea selection and/or subsampling (allows handling large files); Simultaneous display of multiple images/files; Plots intensity profiles along any of the three axes in a 3D data set; Writes displayed data fields to binary files (allows doing further processing using ISIS programs or using other software packages); Reports cursor location and pixel value (includes reporting Latitude/Longitude with optional conversion between geocentric and geographic coordinates); Includes many additional flexible display options. The "cv" program is implemented in the IDL language and makes use of the IDL CALL_EXTERNAL capability to call I/O and utility routines written in C and Fortran. Pre-compiled versions of "cv" are available for Sun Solaris, Compaq Alpha and PC Linux platforms. To obtain "cv", go to the USGS anonymous ftp site ( Do a "cd dist/isis" and get (in binary mode) the README_CV.TXT file (installation instructions) and the tar file for the desired platform (cv_sun.tar, cv_alpha.tar, cv_pc.tar). More information on ISIS is available at the ISIS website ( References: [1] Torson, J.M., and Becker, K.J., (1997), ISIS - A Software Architecture for Processing Planetary Images, Lunar and Plan. Sci. XXVIII, 387-388, [2] Gaddis, L., (1996), Use of ISIS for Processing and Analysis of Planetary Remote Sensing data, GSA Abstracts with Programs, v. 28, No. 7, p. A-286.

Torson, J. M.



Fusion PCR-targeted tylCV gene deletion of Streptomyces fradiae for producing desmycosin, the direct precursor of tilmicosin  

Microsoft Academic Search

Tilmicosin is a semisynthetic macrolide antibiotic derived from tylosin. The disruption of tylCV gene from tylosin producer—Streptomyces fradiae would result in the accumulation of desmycosin, the direct precursor of tilmicosin. TylCV gene disruption cassette was constructed by fusion PCR. The tylCV replacement strain of S. fradiae was obtained through intergeneric conjugation between S. fradiae and E. coli. The antibiotic resistance

Yong Min; Heping Lv; Yinghua Zheng



Mineralogy and Petrography of MIL 090001, a Highly Altered CV Chondrite from the Reduced Sub-Group  

NASA Technical Reports Server (NTRS)

MIL 090001 is a large (greater than 6 kg) CV chondrite from the reduced subgroup (CV(sub red)) that was recovered during the 2009-2010 ANSMET field season [1]. The CV(sub red) subgroup meteorites retain primitive characteristics and have escaped the Na and Fe meta-somatism that affected the oxidized (CV(sub ox)) subgroups. MIL 090001 is, however, reported to be altered [1], and thus a major objective of this study is to characterize its mineralogy and petrography and the extent of the alteration.

Keller, Lindsay P.



Segmentation of kidney using C-V model and anatomy priors  

NASA Astrophysics Data System (ADS)

This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia



CV-990 Landing Systems Research Aircraft (LSRA) during final Space Shuttle tire test  

NASA Technical Reports Server (NTRS)

A Convair 990 (CV-990) was used as a Landing Systems Research Aircraft (LSRA) at NASA's Dryden Flight Research Center, Edwards, California, to test space shuttle landing gear and braking systems as part of NASA's effort to upgrade and improve space shuttle capabilities. The first flight at Dryden of the CV-990 with shuttle test components occurred in April 1993, and tests continued into August 1995, when this photo shows a test of the shuttle tires. The purpose of this series of tests was to determine the performance parameters and failure limits of the tires. This particular landing was on the dry lakebed at Edwards, but other tests occurred on the main runway there. The CV-990, built in 1962 by the Convair Division of General Dynamics Corp., Ft. Worth, Texas, served as a research aircraft at Ames Research Center, Moffett Field, California, before it came to Dryden.



An amoeboid olivine inclusion (AOI) in CK3 NWA 1559, comparison to AOIs in CV3 Allende, and the origin of AOIs in CK and CV chondrites  

NASA Astrophysics Data System (ADS)

An amoeboid olivine inclusion in CK3 NWA 1559 (0.54 × 1.3 mm) consists of a diopside-rich interior (approximately 35 vol%) and an olivine-rich rim (approximately 65 vol%). It is the first AOI to be described in CK chondrites; the apparent paucity of these inclusions is due to extensive parent-body recrystallization. The AOI interior contains irregular 3-15 ?m-sized Al-bearing diopside grains (approximately 70 vol%), 2-20 ?m-sized pores (approximately 30 vol%), and traces of approximately 2 ?m plagioclase grains. The 75-160 ?m-thick rim contains 20-130 ?m-sized ferroan olivine grains, some with 120º triple junctions. A few coarse (25-50 ?m-sized) patches of plagioclase with 2-18 ?m-thick diopside rinds occur in several places just beneath the rim. The occurrence of olivine rims around AOI-1 and around many AOIs in CV3 Allende suggests that CK and CV AOIs formed by the acquisition of porous forsteritic rims around fine-grained, rimless CAIs that consisted of diopside, anorthite, melilite, and spinel. Individual AOIs in carbonaceous chondrites may have formed after transient heating events melted their olivine rims as well as portions of the underlying interiors. In AOI-1, coarse plagioclase grains with diopside rinds crystallized immediately below the olivine rim. Secondary parent-body alteration transformed forsterite in the rims of CV and CK AOIs into more-ferroan olivine. Some of the abundant pores in the interior of AOI-1 may have formed during aqueous alteration after fine-grained melilite and anorthite were leached out. Chondrite groups with large chondrules tend to have large AOIs. AOIs that formed in dust-rich nebular regions (where CV and CK chondrites later accreted) tend to be larger than AOIs from less-dusty regions.

Rubin, Alan E.



Acute overdose of a new dopamine agonist, CV 205-502.  


CV 205-502 is a new dopamine agonist used for hyperprolactinaemia. We report a case of acute overdose (one month treatment, i.e. 2.25 mg) in a 25-year-old male patient. Clinical symptoms were restricted to nausea and mild hypotension. Treatment consisting of ipeca cuanha, charcoal and intravenous fluids allowed a rapid, successful outcome. This case illustrates the particular features of CV 205-502 intoxication when compared to other dopamine agonists, pointing out the tolerance of the drug which can be considered for wider indications and safer prescriptions. PMID:7910531

Tauveron, I; Gesta, J M; Jalenques, I; Thieblot, P



Shelf life of minimally processed apple (cv. Jonagored) determined by colour changes  

Microsoft Academic Search

Physical, chemical and sensory changes of cut apple (cv. Jonagored) stored in the dark at 4 °C were evaluated. Colour was found to be the critical parameter for this product. Apple cubes underwent severe surface browning primarily during the initial days of storage. The shelf-life of cut apple was therefore very limited, to three days maximum.Sensory analyses and objective quality

A. M. C. N Rocha; A. M. M. B Morais



Dr. Matas Alvarado (CV breve) Investigador Titular del Departamento de Computacin del CINVESTAV; Miembro Regular de  

E-print Network

Dr. Matías Alvarado (CV breve) Investigador Titular del Departamento de Computación del CINVESTAV. JOS� MATÍAS ALVARADO MENTADO Curriculum Vitae DATOS PROFESIONALES Investigador CINVESTAV 3B (Febrero JCR ISI 1. Alvarado Matias, Sheremetov, L., Bañares, R. & Cantú, F. (Guest Eds.) Special Issue

Alvarado, Matías


Kenmerk: CvB 2008/879 Afspraken rond invoering UFO  

E-print Network

Kenmerk: CvB 2008/879 Afspraken rond invoering UFO hulpinstrument voor secretaressefuncties Rond de invoering van een UFO hulpinstrument voor de indeling van secretaressefuncties hebben het College van vergemakkelijken. Op de beleids- en uitvoeringsafspraken van UFO heeft dit geen effect; het is een additioneel

Franssen, Michael


Intersimple sequence repeat markers for molecular characterization of Crocus cartwrightianus cv. albus  

Microsoft Academic Search

The Crocus genus, which comprehends approximately 80 species, is known mainly for the triploid sterile plant C. sativus, whose dried stigmas provide us with the spice called saffron. Among the species belonging to the C. sativus L. aggregate, C. cartwrightianus cv. albus shares morphological and cytological characters with both C. sativus and C. cartwrightianus, although its positions seem to be

Angela Rubio Moraga; Almudena Trapero-Mozos; Lourdes Gómez-Gómez; Oussama Ahrazem



Transpiration, crop coefficient and water use of Olive tree (cv. Cordovil) in Southern Portugal  

Microsoft Academic Search

Orchard olive transpiration, soil water status and stomatal response in relation to water deficit were investigated to clarify mechanisms of tree water uptake and stomatal control to improve the irrigation scheduling of low-density olive trees of cv. Cordovil grown in typical Mediterranean environment of Southern Portugal. Trees were subject to three irrigation treatments. Treatment A received 100% of crop evapotranspiration

F. L. Santos



Plant regeneration from cultured protoplasts of the cooking banana cv. Bluggoe ( Musa spp., ABB group)  

Microsoft Academic Search

Summary  Suspensions of embryogenic cells of a triploid banana (Musa spp., cv. Bluggoe) were initiated from the uppermost part of meristematic buds, and used as protoplast source. After 20 weeks in culture, the suspension contained a mixture of globular structures or globules and embryogenic cell clusters, as well as single cells. Two types of protoplasts were obtained from embryogenic suspension culture:

R. Megia; R. Haïcour; S. Tizroutine; V. Bui Trang; L. Rossignol; D. Sihachakr; J. Schwendiman



Alice Alipour CV -Page 1 of 5 Alice Azadeh Alipour, Ph.D., P.E.  

E-print Network

of Civil and Environmental Engineering 234A Marston Hall, 130 Natural Resources Road University networks Damage identification and condition assessment #12;Alice Alipour CV - Page 2 of 5 * indicates Engineering 2007 to 2010 University of California, Irvine Dissertation: "Life-cycle performance assessment

Massachusetts at Amherst, University of


OpenCV WebCam Applications in an Arduino-based Rover  

E-print Network

OpenCV WebCam Applications in an Arduino-based Rover Valeria Loscr�i1 , Nathalie Mitton1 , Emilio Arduino-based Rovers with characteristics of re-programmability, modularity in terms of type and number them. In this paper, we build an Arduino-based platform, that presents some im- portant characteristics

Boyer, Edmond


Comparison of Grape Chitinase Activities in Chardonnay and Cabernet Sauvignon with Vitis rotundifolia cv. Fry  

Microsoft Academic Search

Fungal resistance in Euvitis is generally correlated with levels of pathogenesis-related proteins such as chitinase. Vitis rotundifolia is resistant to many pathogens that affect Vitis vinifera; therefore, grape chitinase ac- tivities were compared in V rotundifolia cv. Fry and V vinifera cvs. Cabernet Sauvignon and Chardonnay. Cabernet Sauvignon and Chardonnay chitinase activities were approximately 130-fold and 80-fold higher than Fry

Steven Van Sluyter; Michael J. D; Christopher J. Halkides


Metabolites in cell suspension cultures, calli, and in vitro regenerated organs of Hypericum perforatum cv. Topas  

Microsoft Academic Search

Methanolic extracts from cell suspension cultures, calli, and in vitro regenerated shoots and roots of Hypericum perforatum cv. Topas have been evaluated for their ability to produce active metabolites (hypericins, hyperforins and flavonoids). Biosynthesis of hypericins is connected with the formation of secretory structures (black globules) in regenerated vegetative buds. A further degree of leaf development is necessary to stimulate

Gabriella Pasqua; Pinarosa Avato; Barbara Monacelli; Anna Rita Santamaria; Maria Pia Argentieri



Economics of the Environment R. Quentin Grafton and John C.V. Pezzey  

E-print Network

Chapter 4 Economics of the Environment R. Quentin Grafton and John C.V. Pezzey 1. Nature and Origins of Economics Nature Since our earliest ancestors people have been making economic decisions accumulated wealth, etc. Economics, in its most basic form, is the study of how given these constraints

Pezzey, Jack


Metal accumulation and damage in rice ( cv. Vialone nano) seedlings exposed to cadmium  

Microsoft Academic Search

The effect of exposure to increasing cadmium concentrations was analyzed in rice seedlings (cv. Vialone nano). The highest Cd accumulation was found in roots, mostly in the apoplastic environment. Cd taken up in cells led to an increase in sulfhydryl groups, the appearance of phytochelatins, and formation of electron-dense vacuolar inclusions. The metal-exposure inhibited root growth and also interfered with

Nicoletta Rascio; Francesca Dalla Vecchia; Nicoletta La Rocca; Roberto Barbato; Cristina Pagliano; Marco Raviolo; Cristina Gonnelli; Roberto Gabbrielli



Spectroscopic classification of ASASSN-14cv as a cataclysmic variable in outburst  

NASA Astrophysics Data System (ADS)

We obtained two low-resolution optical spectra of ASASSN-14cv (vsnet-alert 17395, vsnet-alert 17402, vsnet-alert 17404) on June 22.9 UT using a 0.23-m Schmidt-Cassegrain telescope, Lhires III spectrograph configured for low-resolution

Berardi, Paolo; Lester, Tim; Teyssier, Francois



Simple Cp/Cv Resonance Apparatus Suitable for the Physics Teaching Laboratory.  

ERIC Educational Resources Information Center

Describes a resonance apparatus for the measurement of Cp/Cv for different gases. In the apparatus a magnetically supported piston in a vertical cylindrical glass tube containing the gas is forced into oscillation by means of a standard audio signal generator. (Author/GA)

Smith, D. G.




E-print Network

. Since more evaporation occurred and the temperature fell as time passed, calculations suggest that + anorthite) inclusions in CV3 chondrites match those of the highest temperature condensates (e.g., 1500-1450K at 10-3 atm) predicted from thermodynamic calculations to form during cooling of a gas of solar

Grossman, Lawrence


NOTE Communicated by Thomas Dietterich Combined 5 2 cv F Test for Comparing Supervised  

E-print Network

are not drawn independently of each other), then s2 i /2 has a chi-square distribution with one degree from the same set of available data), then M = 5 i=1 s2 i 2 has a chi-square distribution with 5NOTE Communicated by Thomas Dietterich Combined 5 Ã? 2 cv F Test for Comparing Supervised

Alpaydýn, Ethem


Galaxy and Mass Assembly (GAMA): the GAMA galaxy group catalogue (G3Cv1)  

NASA Astrophysics Data System (ADS)

Using the complete Galaxy and Mass Assembly I (GAMA-I) survey covering ˜142 deg2 to rAB= 19.4, of which ˜47 deg2 is to rAB= 19.8, we create the GAMA-I galaxy group catalogue (G3Cv1), generated using a friends-of-friends (FoF) based grouping algorithm. Our algorithm has been tested extensively on one family of mock GAMA lightcones, constructed from ? cold dark matter N-body simulations populated with semi-analytic galaxies. Recovered group properties are robust to the effects of interlopers and are median unbiased in the most important respects. G3Cv1 contains 14 388 galaxy groups (with multiplicity ?2), including 44 186 galaxies out of a possible 110 192 galaxies, implying ˜40 per cent of all galaxies are assigned to a group. The similarities of the mock group catalogues and G3Cv1 are multiple: global characteristics are in general well recovered. However, we do find a noticeable deficit in the number of high multiplicity groups in GAMA compared to the mocks. Additionally, despite exceptionally good local spatial completeness, G3Cv1 contains significantly fewer compact groups with five or more members, this effect becoming most evident for high multiplicity systems. These two differences are most likely due to limitations in the physics included of the current GAMA lightcone mock. Further studies using a variety of galaxy formation models are required to confirm their exact origin. The G3Cv1 catalogue will be made publicly available as and when the relevant GAMA redshifts are made available at .

Robotham, A. S. G.; Norberg, P.; Driver, S. P.; Baldry, I. K.; Bamford, S. P.; Hopkins, A. M.; Liske, J.; Loveday, J.; Merson, A.; Peacock, J. A.; Brough, S.; Cameron, E.; Conselice, C. J.; Croom, S. M.; Frenk, C. S.; Gunawardhana, M.; Hill, D. T.; Jones, D. H.; Kelvin, L. S.; Kuijken, K.; Nichol, R. C.; Parkinson, H. R.; Pimbblet, K. A.; Phillipps, S.; Popescu, C. C.; Prescott, M.; Sharp, R. G.; Sutherland, W. J.; Taylor, E. N.; Thomas, D.; Tuffs, R. J.; van Kampen, E.; Wijesinghe, D.



Slurry sampling in serum blood for mercury determination by CV-AFS.  


The heavy metal mercury (Hg) is a neurotoxin known to have a serious health impact even at relatively low concentrations. A slurry method was developed for the sensitive and precise determination of mercury in human serum blood samples by cold vapor generation coupled to atomic fluorescence spectrometry (CV-AFS). All variables related to the slurry formation were studied. The optimal hydrochloric concentration and tin(II) chloride concentration for CV generation were evaluated. Calibration within the range 0.1-10 microg L(-1) Hg was performed with the standard addition method, and compared with an external calibration. Additionally, the reliability of the results obtained was evaluated by analyzing mercury in the same samples, but submitted to microwave-assisted digestion method. The limit of detection was calculated as 25 ng L(-1) and the relative standard deviation was 3.9% at levels around of 0.4 microg L(-1)Hg. PMID:18572311

Aranda, Pedro R; Gil, Raúl A; Moyano, Susana; De Vito, Irma; Martinez, Luis D



Cloning, expression and immunolocalization pattern of a cinnamyl alcohol dehydrogenase gene from strawberry (Fragariaxananassa cv. Chandler)  

Microsoft Academic Search

Cinnamyl alcohol dehydrogenase (CAD; EC catalyses the conversion of p-hydroxy-cinnamalde- hydes to the corresponding alcohols and is con- sidered a key enzyme in lignin biosynthesis. By a differential screening of a strawberry (Fragaria3 ananassa cv. Chandler) fruit specific subtractive cDNA library, a full-length clone corresponding to a cad gene was isolated (Fxacad1). Northern blot and quantitative real time PCR

R. Blanco-Portales; N. Medina-Escobar; J. A. Lopez-Raez; J. A. Gonzalez-Reyes; J. M. Villalba; E. Moyano; J. L. Caballero; J. Munoz-Blanco



Influence of edible coating on quality of plum ( Prunus salicina Lindl. cv. ‘Sapphire’)  

Microsoft Academic Search

Edible coating may enhance the boundary layer resistance resulting in enhanced shelf life of fruits. Plums (Prunus salicina Lindl. cv. ‘Sapphire’) were treated with coating material based on carbohydrate (Versasheen) with sorbitol as plasticizer\\u000a and stored at 20 °C and 85% RH. The influence of coating on the gas transmission rates was estimated using a carrier of 100%\\u000a cellulose paper. Coating

Hyang Lan Eum; Dae Keun Hwang; Manfred Linke; Seung Koo Lee; Manuela Zude



Effect of potassium starvation on the uptake of radiocaesium by spring wheat (Triticum aestivum cv. Tonic)  

Microsoft Academic Search

Short term experiments were carried out to investigate the effect of internal tissue potassium concentration on the uptake\\u000a of radiocaesium by spring wheat (Triticum aestivum cv. Tonic). The results showed that potassium starvation increased Cs influx rates by a factor of 10 compared with non-starved\\u000a plants. Solution to plant tissue transfer factor (TF) values also increased by around an order

Y.-G. Zhu; G. Shaw; A. F. Nisbet; B. T. Wilkins



Evaluation of abiotic stress tolerance of genetically modified potatoes ( Solanum tuberosum cv. Desiree)  

Microsoft Academic Search

Abiotic stresses such as drought and extremes of temperature commonly reduce both yield and quality of potato. This study\\u000a investigated the potential to use gene transfer technology to enhance the tolerance of potato to commonly encountered abiotic\\u000a stresses. Agrobacterium mediated transformation was used to create lines of potato (cv. Desiree) that over-expressed either a wheat mitochondrial\\u000a Mn superoxide dismutase (SOD3:1),

D. Waterer; Nicole T. Benning; Guohai Wu; Ximing Luo; Xunjia Liu; Michael Gusta; Alan McHughen; Lawrence V. Gusta



Gibberellins and bud break, vegetative shoot growth and flowering in Metrosideros collina cv. Tahiti  

Microsoft Academic Search

Applications of the growth promotive gibberellins (GAs) GA4 and 2,2-dimethyl GA4, and of C-16,17 endo-dihydro GA5, which is known to promote flowering while inhibiting stem growth in the long-day grass Lolium temulentum, were made to micropropagated plants of Metrosideros collina cv. Tahiti, a highly ornamental cultivar with an intermittent flowering pattern. Gibberellin A4 and 2,2-dimethyl GA4 stimulated vegetative growth both

J. Clemens; P. E. Jameson; P. Bannister; R. P. Pharis



Acylated anthocyanins and flavonols from purple flowers of Dendrobium cv. ‘Pompadour’  

Microsoft Academic Search

Two rare anthocyanins, cyanidin 3-(6-malonylglucoside)-7,3?-di(6-sinapylglucoside) and the demalonyl derivative, were characterised as the purple floral pigments of Dendrobium cv. ‘Pompadour’. Nine known flavonol glycosides were also identified, including the 3-rutinoside-7-glucosides of kaempferol and quercetin. One new glycoside was detected: the ferulyl ester of quercetin 7-rutinoside-7-glucoside. These flavonoid patterns are typical for plants in the family Orchidaceae.

C. A Williams; J Greenham; J. B Harborne; J.-M Kong; L.-S Chia; N.-K Goh; N Saito; K Toki; F Tatsuzawa



Antioxidant activity of polyphenols from solid olive residues of c.v. Coratina  

Microsoft Academic Search

The antioxidant profile of extracts from solid olive residue (SOR) of c.v. Coratina, a cultivar widely diffused in the south of Italy, using both cell-free and cell-based experimental models, was investigated. A total hydroalcoholic extract (polyphenols content 19.7%) and a purified extract (Oleaselect™) (polyphenols content 35.1%) were tested for their ability to quench the stable free radical DPPH, the peroxyl

G. Aldini; A. Piccoli; G. Beretta; P. Morazzoni; A. Riva; C. Marinello; R. Maffei Facino



Establishment of callus and cell suspension cultures of raspberry (Rubus idaeus cv. Royalty)  

Microsoft Academic Search

Callus cultures were initiated from leaf sections of raspberry (Rubus idaeus L.) cv. Royalty. Explants from younger leaves produced significantly more calli than those from older leaves. Anderson's salt mixture was more efficient for callus induction than the Murashige-Skoog medium. The best propagation and growth of calli was observed on Anderson's medium supplemented with 9 µM 2,4-dichlorophenoxyacetic acid, 4.9 µM

Wledzimierz Berejsza-Wysecki; Geza Hrazdin



Cellulase, Fruit Softening and Abscission in Red Raspberry Rubus idaeus L. cv Glen Clova  

Microsoft Academic Search

The ripening of raspberry fruit (Rubus ideausL. cv Glen Clova) is associated with a climacteric rise in ethylene production. As the fruit pigments change from green to red there is a progressive softening, loss of skin strength and a breakdown of cell walls in the mesocarp. An increase in cellulase (endo-1,4-?-d-glucanase) in both drupelets and receptacles accompanies these changes. The




Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis  

Microsoft Academic Search

A protocol for plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis was developed. Viable protoplasts were isolated from embryogenic cell suspensions\\u000a at a yield of 1.2 × 107 protoplasts\\/ml packed cell volume (PCV). Liquid and feeder layer culture systems with medium-A and medium-B were used for\\u000a protoplast culture. In liquid culture system, medium-B was more efficient for

Wang Xiao; Xue-Lin Huang; Xia Huang; Ya-Ping Chen; Xue-Mei Dai; Jie-Tang Zhao



Effect of fermentation conditions on the antioxidant compounds and antioxidant capacity of Lupinus angustifolius cv. zapaton  

Microsoft Academic Search

To study the effect of fermentation on the antioxidant compounds and antioxidant capacity of Lupinus angustifolious cv. zapaton, two different types of fermentation processes were performed. Solid-state fermentations in cracked seeds carried out by\\u000a Aspergillus oryzae, Rhizopus oryzae and Bacillus subtilis and liquid state fermentations in flour and cracked seeds carried out by the microbial population present in the seed

Rebeca Fernandez-Orozco; Juana Frias; Rosario Muñoz; Henryk Zielinski; Mariusz K. Piskula; Halina Kozlowska; Concepción Vidal-Valverde



Thidiazuron-induced plant regeneration from hypocotyl cultures of St. John's wort (Hypericum perforatum. cv 'Anthos')  

Microsoft Academic Search

St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development\\u000a of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N?-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hypocotyl segments of St. John's wort seedlings. The optimum level of thidiazuron supplementation to\\u000a the culture medium

S. J. Murch; K. L. Choffe; J. M. R. Victor; T. Y. Slimmon; S. KrishnaRaj; P. K. Saxena



The relationship questionnaire-clinical version (RQ-CV): Introducing a profoundly-distrustful attachment style  

Microsoft Academic Search

Cost-efficient prenatal assessments are needed that have the potential to identify those at risk for parent\\/infant relational problems. With this goal in mind, an additional attachment style descrip- tion was added to the Relationship Questionnaire Bartholomew & Horowitz, 1991, an established self-report attachment measure, to create the Relationship Questionnaire: Clinical Version RQ-CV. The additional description represents a profoundly-distrustful attachment style:

Bjarne M. Holmes; Karlen Lyons-Ruth



Stereoselective oxidation of racemic 1-arylethanols by basil cultured cells of Ocimum basilicum cv. Purpurascens.  


The biotransformation of racemic 1-phenylethanol (30 mg) with plant cultured cells of basil (Ocimum basilicum cv. Purpurascens, 5 g wet wt) by shaking 120 rpm at 25 degrees C for 7 days in the dark gave (R)-(+)-1-phenylethanol and acetophenone in 34 and 24% yields, respectively. The biotransformation can be applied to other 1-arylethanols and basil cells oxidized the (S)-alcohols to the corresponding ketones remaining the (R)-alcohols in excellent ee. PMID:18060603

Itoh, Ken-ichi; Nakamura, Kaoru; Utsukihara, Takamitsu; Sakamaki, Hiroshi; Horiuchi, C Akira



Effects of cycocel on growth and seed yield of Lotus uliginosus Schk. cv. Grasslands Maku  

Microsoft Academic Search

Field experiments were conducted in two successive seasons to investigate the effects of the plant growth retardant cycocel applied at 1.25 and 2.50 kg a.i.\\/ha at different plant growth stages on the vegetative and reproductive growth, yield components, and seed yield of Lotus uliginosus Schk. cv. Grasslands Maku. Cycocel did not retard internode length or affect total dry matter, flowering

R. S. Tabora; J. G. Hampton



The CV, GV and IV techniques as a tool for the study of real semiconductor surfaces  

Microsoft Academic Search

Capacitance (C-V), energy-loss (G-V) and current (I-V) field-effect modifications have been successfully adapted for studying real semiconductor surfaces. This became possible by the preparation of field-effect capacitors with a mica spacer as a dielectric. The (111) planes of silicon with hole concentration 1012 mm-3 were probed. In general, the capacitors behave just like the classical MOS (MIS) systems with a

L. Szaro



Genetic transformation of Cavendish banana ( Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment  

Microsoft Academic Search

An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature\\u000a male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or

D. K. Becker; B. Dugdale; M. K. Smith; R. M. Harding; J. L. Dale



Headspace-SPME applied to varietal volatile components evolution during Vitis vinifera L. cv. ‘Baga’ ripening  

Microsoft Academic Search

Grape berries of Vitis vinifera L. cv. Baga were collected during 7 weeks since half-véraison to a post-maturation stage. Two vineyards in different locations in Bairrada Appellation, Pedralvites (Ped) and Colégio (Col) were used. The free varietal and pre-fermentative related volatile compounds that arise in the first 2h after crushing the grapes were followed by Headspace-Solid Phase Microextraction (HS-SPME). Twenty-three

Elisabete Coelho; Sílvia M. Rocha; Ivonne Delgadillo; Manuel A. Coimbra



Extremely Na and Cl-rich chondrule from the CV3 carbonaceous chondrite Allende  

Microsoft Academic Search

We report on a study of Al3509, a large Na- and Cl-rich, radially-zoned object from the oxidized CV carbonaceous chondrite Allende. Al3509 consists of fine-grained ferroan olivine, ferroan Al-diopside, nepheline, sodalite, and andradite, and is crosscut by numerous veins of nepheline, sodalite, and ferroan Al-diopside. Some poorly-characterized phases of fine-grained material are also present; these phases contain no significant H2O.

G. J. Wasserburg; I. D. Hutcheon; J. Aléon; E. C. Ramon; A. N. Krot; K. Nagashima; A. J. Brearley



Antibacterial and Antioxidant Activities of Banana (Musa, AAA cv. Cavendish) Fruits Peel  

Microsoft Academic Search

The fresh green and yellow banana peel of, (Musa, cv. Cavendish) fruits were treated with 70% acetone, which were partitioned with chloroform (CHCl3) and ethyl acetate (EtOAc), sequentially. The antioxidant activities of the extracts were evaluated by using the thiocyanate method, ß-carotene bleaching method and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical elimination. While, antimicrobial activities of the extracts and isolated components were

Matook Saif Mokbel; Fumio Hashinaga


Copper uptake and translocation in chicory ( Cichorium intybus L. cv. Grasslands Puna) and tomato (Lycopersicon esculentum Mill. cv. Rondy) plants grown in NFT system. I. Copper uptake and distribution in plants  

Microsoft Academic Search

The uptake and distribution of copper was examined in chicory (Cichorium intybus L. cv. Grasslands Puna) and tomato (Lycopersicon esculentumMill. cv. Rondy) plants grown in a Nutrient Film Technique System (NFT) with addition of 0.05, 5, 10 and 20 mg Cu L-1. Biomass production of shoots and roots of both chicory and tomato was strongly depressed by Cu concentrations higher

M. T. Liao; M. J. Hedley; D. J. Woolley; R. R. Brooks; M. A. Nichols



Fayalite in the Vigarano CV3 carbonaceous chondrite: Occurrences, formation age and conditions  

NASA Astrophysics Data System (ADS)

We have performed petrographic characterization, 53Mn- 53Cr age determination and thermodynamic stability evaluations of fayalite in Vigarano meteorite that belongs to the reduced subgroup of CV3 chondrites. Vigarano is a breccia consisting of clasts which are separate chondrules surrounded by olivine-rich fine-grained materials. Four out of twenty three explored clasts contain fayalites that represent materials of the Bali-like oxidized subgroup of CV3 chondrites. The fayalites (Fa > 80 ) with grain sizes typically < 20 µm occurs in veins that extend from chondrules into the fine-grained materials. The fayalite commonly coexists with troilite and/or magnetite. The fayalite-bearing veins terminate at the boundaries of clasts. No evidence of strong impact enough to make melt veins is found in materials adjacent to the veins. These observations suggest that the fayalite-bearing veins in the Bali-like clasts formed through aqueous alteration in an asteroid prior to fragmentation and re-accretion to the Vigarano parent body. In saponite-rich fine-grained materials, we also found troilite-magnetite veins, which are similar to the fayalite-bearing veins in morphology. Morphological evidences and thermochemical equilibrium calculations suggest that fayalite replaced magnetite, and that replacement occurred at temperatures < 200 °C and low water/rock mass ratios from 0.07 to 0.18, which represent aqueous to metamorphic transition. Fayalite grains typically show iron-magnesium zoning (fayalite content decreases towards the grain edges). Based on equilibrium models, this zoning may have occurred at increasing temperature. The observed initial ratio of ( 53Mn/ 55Mn) 0 = (2.3 ± 0.5) × 10 - 6 suggests that fayalite formed ~ 5 Ma before the timing when the Mn-Cr system was closed in angrite NWA 4801 and has an absolute age of ~ 4563 ± 1 Ma. The age of fayalite is identical within errors to that in Mokoia and Kaba CV3 chondrites, which belong to the Bali-like oxidized subgroup. The identical age implies that aqueous alteration occurred at the same time in parent asteroids of Bali-like subgroup materials. These fayalite-bearing materials may have been derived from a single CV3 asteroid or from separate CV3 asteroids where aqueous alteration simultaneously occurred.

Jogo, Kaori; Nakamura, Tomoki; Noguchi, Takaaki; Zolotov, Mikhail Yu.



Secondary Mineralization of Components in CV3 Chondrites: Nebular and Asteroidal Models  

NASA Astrophysics Data System (ADS)

Our review of mineralogical variations among CV3 chondrites suggests that all components, chondrules, matrices, and CAIs, were affected by various degrees of secondary mineralization. Chondrules and CAIs are rimmed with fayalitic olivine [1, 2]; metal in all components is oxidized and sulfidized to magnetite, Ni-rich metal and sulfides [3]; silicates in all components are aqueously altered to phyllosilicates [4]; and nepheline, sodalite, wollastonite, and hedenbergite replace primary minerals in CAIs [5]. In those CV3s with altered CAIs, nepheline etc. are also present in chondrule mesostases [6] and in matrices [7]. Correlated occurrences of secondary minerals indicate that they have related origins. CV3 chondrites can be divided into three kinds according to their secondary features. Reduced CV3s (e.g., Efremovka) lack magnetite [8] and show minimal secondary features. Oxidized CV3s [8] generally show all features: those like Mokoia contain minor fayalitic rims, nepheline, etc, whereas those like Allende lack phyllosilicates but contain well developed fayalite rims and abundant nepheline, etc. Allende-like CV3 chondrites also contain abundant plate-like matrix olivine (Fa(sub)45-55). Similarities in chemistry and O isotopic composition and petrographic observations suggest that fayalitic rims and plate-like matrix olivine have related origins [1, 9]. The presence of secondary minerals in all components implies that alteration postdated component formation. The absence of secondary minerals in reduced CV3s indicates that CV3 oxidized formed from CV3 reduced-like material. Oxidized and reduced materials coexist in some breccias indicating a common parent asteroid. Nebular origins are widely accepted for most secondary features. To form fayalitic rims and matrix , Palme and colleagues [10, 11] suggest that chondritic components were briefly exposed to a hot (>1500 K), highly oxidizing nebula with H2O/H2 to about 1. Such an environment could have resulted from vaporization after >1000-fold dust/gas enrichment [11]. Fe-rich olivine will not condense until most Mg has condensed into forsterite [11]. The steep compositional gradients between adjacent fayalite and forsterite limit the duration of fayalite condensation to a period of several hours [2]. There are several inconsistencies in this late-stage evaporation-condensation model. Fayalitic rims occur inside chondrules and formed by alteration, not by condensation. Forsterite and enstatite grains that supposedly condensed from the nebula are absent on chondrule rims and in chondrites. Magnetite, Ni-rich metal and sulfides are present inside matrix olivine, inconsistent with equilibrium calculations. I-Xe data suggest that sodalite formation in Allende lasted for about 10 Myr, which is inconsistent with a nebular origin [12]. Asteroidal alteration is favored for magnetite [3] and required for most phyllosilicates [4]. Asteroidal formation of fayalite [13] was rejected [2], partly because hydrous minerals are absent in Allende. We suggest that Allende-like CV3 chondrites may have formed in an asteroid by aqueous alteration and dehydration; see Krot et al. [this volume] for details. Higher Na and K concentrations in oxidized CV3 chondrites are not inconsistent with asteroidal alteration, as CM2 chondrites show similar heterogeneities. Acknowledgments: This work was supported by NASA grants NAGW-3281 (K. Keil) and 152-11-40-23 (M.E.Z.). References: [1] Peck J. A. and Wood J. A. (1987) GCA, 51, 1503-1510. [2] Hua X. et al. (1988) GCA, 52, 1389-1408. [3] Blum J. D. et al. (1989) GCA, 53, 543-556. [4] Keller L. P. et al. (1994) GCA, 58, 5589-5598. [5] Hashimoto A. and Grosman L. (1987) GCA, 51, 1685-1704. [6] Kimura M. and Ikeda Y. (1992) Proc. Symp. Antarc. Meteorites, 17, 31-33. [7] Peck J. A. (1983) LPS XIV, 373-374. [8] McSween H. Y. (1977) GCA, 41, 1777-1790. [9] Weinbruch S. et al. (1993) GCA, 57, 2649-2661. [10] Palme et al. (1991) Meteoritics, 25, 383. [11] Palme H. and Fegley B. (1991) EPSL, 101, 180-195. [12] Swindle T. D. et al. (1988) GCA, 52, 2215-2227. [13]

Scott, E. R. D.; Krot, A. N.; Zolensky, M. E.



Automatic Segmentation of the Papilla in a Fundus Image Based on the CV Model and a Shape Restraint  

Microsoft Academic Search

For computer aided Glaucoma diagnostics it is essential to robustly and automatically detect and segment the main regions, e.g. the papilla (optic nerve head), in a fundus image. In this paper an effective method for automatic papilla segmentation based on the C-V model and a shape restraint is proposed. The method is a combination between the C-V model using level

Yandong Tang; Xiaomao Li; Axel Von Freyberg; Gert Goch



ProtCV: A Tool for Extracting, Visualizing and Validating Protein Clusters Using Mass Spectra Peak-Lists  

Microsoft Academic Search

We present ProtCV (protein clustering and visualization) a new software tool for grouping samples (mass spectra peak-lists) emanating from a high throughput proteomics analysis based on their spectral similarities, and summarizing effectively for the user the clustering results using advanced visualization methods. A unique feature of ProtCV is that it can compare clustering methods applied to the same data set

Stavroula Ventoura; Eugenia G. Giannopoulou; Elias S. Manolakos



Complete CV  

E-print Network

the German national merit scholarship program, for doctoral studies. 5. Fellowship of the ... Ad hoc committee for strategic planning (interdiscipliary, equipment). Spr 06 ..... Frobenius Manifolds: Basic constructions and operations. 45. Meeting ...



mdx5cv Mice Manifest More Severe Muscle Dysfunction and Diaphragm Force Deficits than Do mdx Mice  

PubMed Central

Duchenne muscular dystrophy (DMD) is characterized by progressive skeletal muscle dysfunction leading to premature death by the third decade of life. The mdx mouse, the most widely used animal model of DMD, has been extremely useful to study disease mechanisms and to screen new therapeutics. However, unlike patients with DMD, mdx mice have a very mild motor function deficit, posing significant limitations for its use as a platform to assess the impact of treatments on motor function. It has been suggested that an mdx variant, the mdx5cv mouse, might be more severely affected. Here, we compared the motor activity, histopathology, and individual muscle force measurements of mdx and mdx5cv mice. Our study revealed that mdx5cv mice showed more severe exercise-induced fatigue, Rotarod performance deficits, and gait anomalies than mdx mice and that these deficits began at a younger age. Muscle force studies showed more severe strength deficits in the diaphragm of mdx5cv mice compared to mdx mice, but similar force generation in the extensor digitorum longus. Muscle histology was similar between the two strains. Differences in genetic background (genetic modifiers) probably account for these functional differences between mdx strains. Overall, our findings indicate that the mdx and mdx5cv mouse models of DMD are not interchangeable and identify the mdx5cv mouse as a valuable platform for preclinical studies that require assessment of muscle function in live animals. PMID:21893021

Beastrom, Nicholas; Lu, Haiyan; Macke, Allison; Canan, Benjamin D.; Johnson, Eric K.; Penton, Christopher M.; Kaspar, Brian K.; Rodino-Klapac, Louise R.; Zhou, Lan; Janssen, Paul M.L.; Montanaro, Federica



The first mass and angular momentum loss measurements for a CV-like binary  

NASA Astrophysics Data System (ADS)

The period distribution of close binaries, cataclysmic variables, novae and single-degenerate SN1a progenitor candidates is largely controlled by magnetically-driven mass and angular momentum loss (AML) from the M dwarf secondary. The mass loss rates for these spun-up stars remain essentially unknown and impossible to observe directly, with likely values in the range 1e-12 to 1e-15 Msun/yr. AML presciptions for CVs differ by orders of magnitude. One way to measure the mass loss rate is to observe the dM wind accrete onto its WD companion in a pre-CV very close to Roche Lobe overflow but lacking the obscuring complications and emission from an accretion disk. The measurement can be combined with realistic MHD models to understand the accretion fraction, the mass that escapes, and the AML. The best-studied nearby pre-CV is QS Vir (48pc, P=3.6hr). However, its wind accretion rates measured from 1999 HST UV spectra of the WD metal absorption lines and 2006 XMM-Newton CCD spectroscopy differ by a factor of a thousand, pointing to either a dominant CME stochastic component, or a "magnetic switch" found in MHD simulations and driven by cyclic activity on the M dwarf. HST COS spectra combined with XMM-Newton monitoring on timescales from weeks to years will tease out CME vs cyclic accretion variations. UV and X-ray measurements will provide the first consistency check of both accretion rate measurement methods. MHD models tailored to the system will enable the first quasi-direct measurements of the mass loss and AML from a CV-like binary. Our project requires 6 HST/COS orbits in Cycles 22-24, and 60ksec on XMM in Cycle 22

Drake, Jeremy



Dark inclusions in Allende, Leoville, and Vigarano - Evidence for nebular oxidation of CV3 constituents  

NASA Astrophysics Data System (ADS)

The origin and the history of dark inclusions (DIs) are investigated using petrologic, chemical, and oxygen isotopic data on ten DI samples from Allende, Leoville, and Vigarano. These data indicate that the DIs of the Leoville and Vigarano are closely similar to those of Allende. The inclusions appear to be fragments of CV3 parent bodies which were processed to different degrees prior to their incorporation as clasts into the Allende, Leoville, and Vigarano chondrites. The processing homogenized the olivine compositions, presumably through heating, and also involved oxygen exchange with O-16-poorer surroundings.

Johnson, C. A.; Prinz, M.; Weisberg, M. K.; Clayton, R. N.; Mayeda, T. K.



Efficient, direct plant regeneration from stem segments of chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple).  


Direct plant regeneration was obtained from fresh chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple) stem segments cultured on Murashige and Skoog's (1962) basal media supplemented with 6-benzylaminopurine (BAP, 0.5-2.0 mg/l) and ?-naphthaleneacetic acid (NAA, 0.2-2.0 mg/l). The morphogenetic potential varied with the developmental stage of the stem explant. The highest percentage of shoot formation (100%) and greatest average number of shoots per explant (14.6) were observed on stem segments taken from the top of the cutting. This organogenetic capacity decreases in the more mature stem. Normal, flowering plants were obtained three to four months after culture. PMID:24232929

Lu, C Y; Nugent, G; Wardley, T



Partial purification and characterization of peroxidase from olives ( Olea europaea cv. Koroneiki)  

Microsoft Academic Search

The enzyme peroxidase (POD) activity was extracted from olives (Olea europaea cv. Koroneiki) and was partially purified by ammonium sulfate fractionation and gel permeation chromatography (Sephacryl\\u000a S 300). Further characterization of the POD was performed using the ammonium sulfate purified fraction. POD showed a molecular\\u000a mass of 44 ± 2 kDa and it expressed catalytic activity with 2,2?-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS),\\u000a N,N-dimethyl-p-phenylenediamine (DMPD) and

Evaggelia D. Tzika; Theodore G. Sotiroudis; Vassiliki Papadimitriou; Aristotelis Xenakis



Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO.  


Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity of the tissues involved. Factors causing these variations were investigated by climate chamber (CC) experiments focusing on temperature and ultraviolet (UV)-B radiation. Plants of A. montana L. cv. ARBO were grown in CCs under realistic climatic and radiation regimes. Key factors temperature and UV-B radiation were altered between different groups of plants. Subsequently, flowering heads were analyzed by HPLC for their contents of flavonoids and caffeic acid derivatives. Surprisingly, increased UV-B radiation did not trigger any change in phenolic metabolites in Arnica. In contrast, a pronounced increase in the ratio of B-ring ortho-diphenolic (quercetin) compared to B-ring monophenolic (kaempferol) flavonols resulted from a decrease in temperature by 5 degrees C in the applied climate regime. In conclusion, enhanced UV-B radiation is probably not the key factor triggering shifts in the phenolic composition in Arnica grown at higher altitudes but rather temperature, which decreases with altitude. PMID:19194724

Albert, Andreas; Sareedenchai, Vipaporn; Heller, Werner; Seidlitz, Harald K; Zidorn, Christian



Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN  

PubMed Central

We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured under escalating concentrations of these lectins became increasingly resistant tolerating 2 to 12 times their 50% inhibitory concentrations. Sequence analysis of gp120 showed that most had deletions of 1 to 5 mannose-rich glycans. Glycosylation sites at positions 230, 234, 241, 289 located in the C2 region and 339, 392 and 448 in the C3-C4 region were affected. Furthermore, deletions and insertions of up to 5 amino acids in the V4 region were observed in 3 of the 4 isolates. These data suggest that loss of glycosylation sites on gp120 as well as rearrangement of glycans in V4 are mechanisms involved in HIV-1 subtype C escape from GRFT, CV-N and SVN. PMID:24074568

Alexandre, Kabamba B.; Moore, Penny L.; Nonyane, Molati; Gray, Elin S.; Ranchobe, Nthabeleng; Chakauya, Ereck; McMahon, James B.; O’Keefe, Barry R.; Chikwamba, Rachel; Morris, Lynn



Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds.  


Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative identification of the compounds present in the lowest and highest yield extracts. Analysis of the leaf extract with phytochemical tests showed the presence of alkaloids, flavonoids and terpenes. Antifungal effectiveness was determined by challenging the extracts (LE, SRE, SUE) from the best extraction treatment against three phytopathogenic fungi: Rhizopus stolonifer, Fusarium spp. and Colletotrichum gloeosporioides. The leaf extract exhibited the broadest action spectrum. The MIC(50) for the leaf extract was 0.625 mg ml(-1) for Fusarium spp. and >10 mg ml(-1) for C. gloeosporioides, both equal to approximately 20% mycelial growth inhibition. Ethanolic extracts from Carica papaya L. cv. Maradol leaves are a potential source of secondary metabolites with antifungal properties. PMID:22282629

Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago



Antioxidant and Anticancer Aporphine Alkaloids from the Leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena.  


Fifteen compounds were extracted and purified from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena. These compounds include liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) cepharadione B (11), ?-sitostenone (12), stigmasta-4,22-dien-3-one (13) and two chlorophylls: pheophytin-a (14) and aristophyll-C (15). The anti-oxidation activity of the compounds was examined by antiradical scavenging, metal chelating and ferric reducing power assays. The results have shown that these compounds have antioxidative activity. The study has also examined the antiproliferation activity of the isolated compounds against human melanoma, prostate and gastric cancer cells. The results shown that 7-hydroxydehydronuciferine (10) significantly inhibited the proliferation of melanoma, prostate and gastric cancer cells. Together, these findings suggest that leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena are a good resource for obtaining the biologically active substances with antioxidant properties. PMID:25372397

Liu, Chi-Ming; Kao, Chiu-Li; Wu, Hui-Ming; Li, Wei-Jen; Huang, Cheng-Tsung; Li, Hsing-Tan; Chen, Chung-Yi



Electroacupuncture Stimulation at CV12 Inhibits Gastric Motility via TRPV1 Receptor  

PubMed Central

Gastric dysmotility is one of the major pathophysiological factors in functional gastrointestinal disorders. Acupuncture, as one of the alternative approaches, is efficacious in the treatment of gastrointestinal motility disorders; however, the mechanism underlying its action is unclear. In the present study, we used both capsazepine, a TRPV1 antagonist, and TRPV1 knockout mice. Animals were divided into wild-type group (WT), capsazepine injection group (CZP, 0.5?mg/kg, i.p.), and TRPV1 knockout mice group (TRPV1?/?). Each of these three groups was divided into three subgroups, which were subjected to EA stimulation at acupoint Zhongwan (CV12) at a different intensity (1, 2, or 4?mA). We demonstrated that electroacupuncture at Zhongwan (CV12) markedly inhibited gastric motility at 2 and 4?mA in an intensity-dependent manner in wild-type mice. The inhibitory effect was also observed in capsazepine-injected and TRPV1?/? mice but was no longer intensity dependent, indicating that TRPV1 is partially involved in the electroacupuncture-mediated modulation of gastric motility. PMID:24106521

Yu, Zhi; Cao, Xin; Xia, Youbing; Ren, Binbin; Feng, Hong; Wang, Yali; Jiang, Jingfeng; Xu, Bin



Transient Extremely Soft X-ray Emission from the Unusually Bright CV in the Globular Cluster M3: a New CV X-ray Luminosity Record?  

E-print Network

We observed the accreting white dwarf 1E1339.8+2837 (1E1339) in the globular cluster M3 in Nov. 2003, May 2004 and Jan. 2005, using the Chandra ACIS-S detector. The source was observed in 1992 to possess traits of a supersoft X-ray source (SSS), with a 0.1-2.4 keV luminosity as large as 2x10^{35} erg/s, after which time the source's luminosity fell by roughly two orders of magnitude, adopting a hard X-ray spectrum more typical of CVs. Our observations confirm 1E1339's hard CV-like spectrum, with photon index Gamma=1.3+-0.2. We found 1E1339 to be highly variable, with a 0.5-10 keV luminosity ranging from 1.4+-0.3x10^{34} erg/s to 8.5+4.9-4.6x10^{32} erg/s, with 1E1339's maximum luminosity being perhaps the highest yet recorded for hard X-ray emission onto a white dwarf. In Jan. 2005, 1E1339 displayed substantial low-energy emission below 0.3 keV. Although current Chandra responses cannot properly model this emission, its bolometric luminosity appears comparable to or greater than that of the hard spectral comp...

Stacey, W S; Elsner, R F; Edmonds, P D; Weisskopf, M C; Grindlay, J E



Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves.  


The pathogenesis of GIII.2 bovine norovirus (BoNoV) is not well understood. Our study demonstrated persisting diarrhea and prolonged fecal shedding, but with a lack of significant intestinal lesions in gnotobiotic (Gn) calves infected with GIII.2 BoNoV, CV186-OH/00/US strain. Nine 4 to 7-day-old Angus/Jersey crossbred Gn calves were orally inoculated with 10.0-11.9 log10 genomic equivalents (GE)/calf of CV186-OH (n=7) or mock (n=2). Calves were euthanized at post-inoculation day (PID) 1 (n=1) when moderate to severe lethargy was observed and at PIDs 2-6 (n=4) after lethargy had subsided. Two calves were kept longer term (until PID 30) for monitoring fecal shedding patterns by TaqMan real-time RT-PCR (qRT-PCR). Most infected calves exhibited two clinical signs: (i) acute but persisting diarrhea and (ii) acute moderate to severe lethargy. The two infected calves, followed longer-term, had prolonged fecal viral RNA shedding [peak average titer of 11.8 (± 0.2) log10GE/ml] at least until PID 20. By qRT-PCR, 5 infected calves had low viral RNA titers in serum, ranging from 4.0 to 5.8 log10GE/ml, at PIDs 1-5, but not (<2.7 log10GE/ml) at PIDs 6-30. The latter observation coincided with the presence of serum IgG antibody to BoNoV at PIDs 8-30. Collectively, the GIII.2 BoNoV strain CV186-OH induced only mild enteropathogenicity, evident by the lack of significant intestinal lesions, but it led to persisting mild diarrhea and prolonged fecal virus shedding in Gn calves. The prolonged fecal shedding of GIII.2 BoNoV might partially explain how this virus is maintained as endemic infections in cattle. PMID:24309403

Jung, Kwonil; Scheuer, Kelly A; Zhang, Zhenwen; Wang, Qiuhong; Saif, Linda J



The digestion of fresh perennial ryegrass (Lolium perenne L. cv. Melle) and white clover (Trifolium repens L. cv. Blanca) by growing cattle fed indoors.  


1. Pure swards of perennial ryegrass (Lolium perenne L. cv. Melle) or white clover (Trifolium repens L. cv. Blanca) were harvested daily at three and two stages of growth respectively, and offered to housed cattle. The grass diets comprised primary growth (May) and two later regrowths of contrasting morphology (i.e. leaf: stem values of 1.54 and 2.84 respectively), and were characterized by high contents of water-soluble carbohydrate and neutral-detergent fibre and comparable in vitro dry matter (DM) digestibilities (mean 0.80). Total nitrogen content was higher on primary growth grass (34 g/kg DM) than on regrowths (23 g/kg DM) but lower than values obtained for the two clover diets (38 and 43 g/kg DM, respectively). The clover diets had lower water-soluble carbohydrate contents than the grasses, comparable cellulose, but lower neutral-detergent fibre contents and in vitro DM digestibilities of 0.70 and 0.77 respectively. 2. The experiment lasted from May until August, during which time a total of twenty-one young Friesian steers (initial average live weight 130 kg) were used to determine both nutrient supply to the small intestine (twelve animals) and apparent digestibility (nine animals). Each diet was offered at three levels of DM intake (i.e. 18, 22 and 26 g/kg live weight). A further six steers, all fed at the rate of 22 g DM/kg live weight, were used to determine the metabolizable energy contents of the five diets by means of open-circuit calorimetry. 3. The three grass diets and the later-cut clover had, as intended, quite similar in vivo organic matter digestibilities, but that of the earlier-cut clover was lower, and this was associated with a large number of flower heads in this crop at the time of feeding. 4. On the clover diets, proportionately less of the ingested organic matter appeared to be digested in the rumen (0.40) compared with the grass diets (0.58) (P less than 0.001). On the high-N primary grass and the clover diets, substantial rumen losses of N were detected (P less than 0.01) compared with regrowth grasses. 5. The metabolizable energy content of the primary growth of grass was 12.2 MJ/kg DM, whilst the values for the other two grass diets were lower (11.6 MJ/kg DM), despite no marked decline in overall energy digestibility. Values for the two clover diets (mean 10.5 MJ/kg DM) were considerably lower than all values noted for the grasses.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:3870696

Beever, D E; Thomson, D J; Ulyatt, M J; Cammell, S B; Spooner, M C



Altitudinal variation of secondary metabolite profiles in flowering heads of Matricaria chamomilla cv. BONA.  


The altitudinal variation of the contents of secondary metabolites in flowering heads of Matricaria chamomilla L. (Asteraceae) was assessed. Plants of M. chamomilla cultivar BONA were grown in nine experimental plots at altitudes between 590 and 2,230 m at Mount Patscherkofel near Innsbruck/Austria. The amounts of flavonoids and phenolic acids were quantified by HPLC/DAD. For both flavonoids and phenolic acids positive (r = 0.559 and 0.587) and statistically significant (both p < 0.001) correlations with the altitude of the growing site were observed. The results are compared to previous results on Arnica montana L. cv. ARBO. Moreover, various ecological factors, which change with the altitude of the growing site, are discussed as potential causes for the observed variation. PMID:18484542

Ganzera, Markus; Guggenberger, Manuela; Stuppner, Hermann; Zidorn, Christian



Partial melting of the Allende (CV3) meteorite - Implications for origins of basaltic meteorites  

NASA Astrophysics Data System (ADS)

Eucrites and angrites are distinct types of basaltic meteorites whose origins are poorly known. Experiments in which samples of the Allende (CV3) carbonaceous chondrite were partially melted indicate that partial melts can resemble either eucrites or angrites, depending only on the oxygen fugacity. Melts are eucritic if this variable is below that of the iron-wuestite buffer or angritic if above it. With changing pressure, the graphite-oxygen redox reaction can produce oxygen fugacities that are above or below those of the iron-wuestite buffer. Therefore, a single, homogeneous, carbonaceous planetoid greater than 110 kilometers in radius could produce melts of drastically different composition, depending on the depth of melting.

Jurewicz, A. J. G.; Mittlefehldt, D. W.; Jones, J. H.



Proliferation potential of 18-month-old callus of Ananas comosus L. cv. Moris.  


Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of Ananas comosus L. cv. Moris were assessed in vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS) media, 32.22 microM alpha-naphthaleneacetic acid (NAA) gave the highest mean fresh weight of callus (46.817 g). Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) was inferior to NAA, while b-naphthoxy acetic acid (BNOA) and p-chlorophenoxy acetic acid (4-CPA) were not effective in proliferating 18-months old callus. Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus. However, in terms of callus production, NAA at 32.22 microM was economically better. PMID:16493521

De Silva, A E; Kadir, M A; Aziz, M A; Kadzimin, S



Postharvest treatments with ethylene on Vitis vinifera (cv Sangiovese) grapes affect berry metabolism and wine composition.  


Grapes (Vitis vinifera, cv Sangiovese), harvested at standard commercial maturity, were treated for 36 h with ethylene (ET, 1000 ppm) or air (control, CT) before vinification. The composition of the grapes, must and wine was different in the CT and ET samples. In the ET wine, higher concentrations of specific phenol compounds, belonging to the classes of flavonols, anthocyanins, flavan-3-ols, and stilbenes, were detected. ET induced a significant change in the wine aroma profile by increasing free volatile categories such as phenols and fatty acids, and reducing the content of carbonyl compounds and, in particular, of esters. Less pronounced differences between CT and ET wines were observed in terms of glycosidically-bound volatile compounds. The activity of pectin methyl esterase and ?-glucosidase was enhanced in ET-treated berry skins, suggesting that cell wall properties and changes in the hydrolytic activity are effective in modulating the composition of CT and ET wines. PMID:24767053

Becatti, Elisa; Genova, Giuseppe; Ranieri, Annamaria; Tonutti, Pietro



Methyleugenol and eugenol variation in Ocimum basilicum cv. Genovese gigante grown in greenhouse and in vitro.  


The variation of the two main aromatic compounds in Ocimum basilicum cv Genovese Gigante grown in greenhouse and "in vitro" was analyzed. The content of methyleugenol and eugenol was correlated to the plants' height rather than to the plants' age and the growth site. Particularly, methyleugenol was prevalent in plants up to 6.5 cm, as plants grew it was replaced by eugenol that was dominant in taller plants. Analysis of basil 20 cm in height showed that methyleugenol is prevalently localized in the low part while eugenol is prevalent in the upper part of the plant. Moreover, a chronic and acute toxicity of methyleugenol was evidentiated in an assay using chicken embryos. PMID:11822201

Miele, M; Ledda, B; Falugi, C; Mazzei, M



Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv "White Cloud Bean"  

PubMed Central

A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White Cloud Bean”. Both trypsin inhibitors exhibited a molecular mass of 16?kDa and reduced the activity of trypsin with an IC50 value of about 0.6??M. Dithiothreitol attenuated the trypsin inhibitory activity, signifying that an intact disulfide bond is indispensable to the activity. [Methyl-3H] thymidine incorporation by leukemia L1210 cells was inhibited with an IC50 value of 28.8??M and 21.5??M, respectively. They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100??M. PMID:20617140

Sun, Jian; Wang, Hexiang; Ng, Tzi Bun



High floral bud abscission and lack of open flower abscission in Dendrobium cv. Miss Teen: rapid reduction of ethylene sensitivity in the abscission zone  

Microsoft Academic Search

We studied the abscission of floral buds and open flowers in cut Dendrobium inflorescences. Abscission of floral buds was high and sensitive to ethylene in all cultivars studied. Many open flowers abscised in most cultivars, but cv. Willie exhibited only small amount of floral fall and cv. Miss Teen none. Applied ethylene (0.4 ¿L L¿1 for 24 h at 27°C)

Kanokpon Bunya-atichart; Saichol Ketsa; Doorn van W. G



Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan  

PubMed Central

Background We performed an environmental study of viruses infecting the symbiotic single-celled algae of Paramecium bursaria (Paramecium bursaria Chlorella virus, PBCV) in Lake Biwa, the largest lake in Japan. The viruses detected were all Chlorella variabilis virus (CvV = NC64A virus). One of them, designated CvV-BW1, was subjected to further characterization. Results CvV-BW1 formed small plaques and had a linear DNA genome of 370 kb, as judged by pulsed-field gel electrophoresis. Restriction analysis indicated that CvV-BW1 DNA belongs to group H, one of the most resistant groups among CvV DNAs. Based on a phylogenetic tree constructed using the dnapol gene, CvV was classified into two clades, A and B. CvV-BW1 belonged to clade B, in contrast to all previously identified virus strains of group H that belonged to clade A. Conclusions We conclude that CvV-BW1 composes a distinct species within C. variabilis virus. PMID:20831832



CV CHECKLIST FOR PH.D. CANDIDATES Brandeis University Graduate School of Arts and Sciences Career Services  

E-print Network

read the job description in detail and identified all of my key skillsCV CHECKLIST FOR PH.D. CANDIDATES Brandeis University Graduate School of Arts faculty positions), or 1-2 page resumé (some Administration positions) CONTENT

Fraden, Seth


Effect of Three Species of Arbuscular Mycorrhiza on Bud Sprout and Ripening in Grapevine (Vitis vinifera L.) cv. Perlette  

Microsoft Academic Search

Investigations were carried out with grape cv. Perlette to assess the influence of arbuscular mycorrhiza (AM) on breaking bud dormancy, and hastening flowering, berry set and berry ripening. Eighteen year old vines of Perlette, having uniform vigour and growth, planted at 2.5 × 2.5 m and trained on a telephone system, were inoculated with three strains of AM namely Glomus

K. Usha; Rebecca Mathew; B. Singh



Marc Conrad's publications, as of May 2009. Please ask me if you need a full CV or an update.  

E-print Network

Page 1 Marc Conrad's publications, as of May 2009. Please ask me if you need a full CV or an update. Disclaimer: There might be errors and omissions. Papers: 1. Conrad, M., French, T., Maple, C., Huchard, M of Object Technology, 5(6):107­126, July-August 2006. 2. Conrad, M., French, T., Maple, C., Zhang, S

Conrad, Marc


Internship Checklist for DEB students 1. Before you submit a CV and cover letter to any company, please have Dr.  

E-print Network

Internship Checklist for DEB students Feb 2008 1. Before you submit a CV and cover letter to any an internship (in Objective section) and also list the DEB as part of your PhD education. Example: PhD in Chemistry with a Designated Emphasis in Biotechnology. 3. If you are offered an internship, please notify Dr

Ferrara, Katherine W.


Using Multidimensional Rasch Analysis to Validate the Chinese Version of the Motivated Strategies for Learning Questionnaire (MSLQ-CV)  

ERIC Educational Resources Information Center

This article used the multidimensional random coefficients multinomial logit model to examine the construct validity and detect the substantial differential item functioning (DIF) of the Chinese version of motivated strategies for learning questionnaire (MSLQ-CV). A total of 1,354 Hong Kong junior high school students were administered the…

Lee, John Chi-Kin; Zhang, Zhonghua; Yin, Hongbiao



The influence of nitrogen and defoliation on production and water?use efficiency of Lolium multiflorum cv. Midmar  

Microsoft Academic Search

The objective of this study was to investigate the influence of different nitrogen fertiliser application levels in combination with different defoliation intervals on the dry matter (DM) production and water?use efficiency of ryegrass (Lolium multiflorum,) cv. Midmar. In a field trial, four nitrogen levels (0, 150, 300 and 450 kg N ha) were combined with five defoliation intervals (every 2,

J. F. Theron; W. L. J. van Rensburg



Ontogenetic histological changes in the wood of mango ( Mangifera indica L. cv Deshi) exposed to coal-smoke pollution  

Microsoft Academic Search

Trees of Mangifera indica L. cv Deshi growing at two sites, one heavily polluted due to coal-smoke emanating from a thermal power plant and the other free from such pollution, were examined across their boles (from pith to cambium) to trace ontogenetic changes induced by coal-smoke pollutants in the wood structure with special reference to vessels and fibres. Wood formation

M. C. Gupta; M. Iqbal



ELSEVIER Earth and Planetary Science Letters 146 (1997) 337-349 Origin of magnetite in oxidized CV chondrites: in situ  

E-print Network

is somewhat more negative than estimates for the ambient solar nebula gas. We infer that the magnetite formed- netite (and other oxidized phases in the oxidized CV chondrites) occurred in the solar nebula melts [2,3]; (2) oxidation of chondrule metallic nodules in the nebula [4-61; and (3) oxidation


Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg  

E-print Network

Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg GRECO Cvo UNI OL/ Nds.PV-Symp. 06-2009 semiconductor physics / quantum solar energy conversion;Semiconductor Physics and Quantum Solar Energy Conversion Semiconductor Physics and Quantum Solar Energy


Feifei Pan CV--Last updated on September 26, 2012 Page 1 of 7 Department of Geography  

E-print Network

and Climate Change, 2012-present Memberships · American Geophysical Union, Hydrology Section, 1996-presentFeifei Pan CV--Last updated on September 26, 2012 Page 1 of 7 Feifei Pan Department of Geography University of North Texas Denton, TX 76202 (940)369-5109(Phone), (940)369-7550(Fax) Email: feifei.pan

Pan, Feifei


Influence of partial rootzone drying on the composition and accumulation of anthocyanins in grape berries ( Vitis vinifera cv. Cabernet Sauvignon)  

Microsoft Academic Search

Background and Aims: The aim of the study was to investigate anthocyanin composition and accu- mulation in grape berries in response to the partial rootzone drying (PRD) irrigation technique. Method and Results: The experiment was on Vitis vinifera cv. Cabernet Sauvignon, wherein PRD had a 40% water deficit relative to the control treatment. PRD decreased berry weight compared with the




Effect of abscisic acid and cytokinins on cultured zygotic embryos of Coffea arabica cv.Cauvery (Catimor)  

Microsoft Academic Search

Immature zygotic embryos of Coffea arabica (Catimor) were sequentially cultured on different modifications of Murashige and Skoog's (MS) medium to test the effect of abscisic acid and cytokinins. The type of response depended on the medium strength, the growth regulator combinations as well as the period of initial culture in both abscisic acid or cytokinin supplemented media. Increasing concentration

M. Madhava Naidu; C. S. Sreenivasan



Martin O. Saar CV, Page 1 of 20 10/11/2010 Note: Publications are listed toward the end.  

E-print Network

Martin O. Saar CV, Page 1 of 20 10/11/2010 Note: Publications are listed toward the end. CURRICULUM VITAE Martin O. Saar E-mail: Department of Geology and Geophysics Office: +1 612/2005-date George and Orpha Gibson Chair of Hydrogeology and Geofluids, University of Minnesota ­ Twin Cities

Saar, Martin O.


The most abundant protease inhibitor in potato tuber (Cv. Elkana) is a serine protease inhibitor from the Kunitz Family  

Microsoft Academic Search

The gene of the most abundant protease inhibitor in potato cv. Elkana was isolated and sequenced. The deduced amino acid sequence of this gene showed 98% identity with potato serine protease inhibitor (PSPI), a member of the Kunitz family. Therefore, the most abundant protease inhibitor was considered to be one of the isoforms of PSPI. The PSPI group represents ~22%

Laurice Pouvreau; Harry Gruppen; Koningsveld van G. A; Broek van den L. A. M; Alphons G. J. Voragen



Ultrastructural localization of Kunitz inhibitor on thin sections of Glycine max (soybean) cv. Maple Arrow by the gold method  

Microsoft Academic Search

The soybean trypsin inhibitor (SBTI, Kunitz type) was localized by immunofluorescence and, at the ultrastructural level, by the protein A gold method on thin sections of Glycine max (soybean) cv. Maple Arrow. SBTI was localized in cell walls, protein bodies, the cytoplasm between the lipid-containing spherosomes, and the nucleus of the cotyledon and embryonic axis. In the nucleus, SBTI was

M. Horisberger; M. Tacchini-Vonlanthen



Analysis of SSR and AFLP Markers to Detect Genetic Diversity Among Selected Clones of Grapevine (Vitis vinifera L.) cv. Keshmeshi  

Microsoft Academic Search

To assess the genetic differences between clones of grapevine (Vitis vinifera L.) cv. Keshmeshi, ten selected clones from a clonal selection programme were analysed by 23 Simple Sequence Repeats (SSR) and seven AFLP primer combinations. No intra-varietal differences between the clones could be detected by SSRs, whereas eight out of the 499 AFLP fragments generated by the seven primer combinations

H. Doulati Baneh; S. A. Mohammadi; H. Mahmoudzadeh; F. de Mattia; M. Labra


The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface.  


The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is "hijacked" by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 "self"-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A; Bianchet, Mario A; Wang, Lai-Xi; Wilson, Iain B H; Vasta, Gerardo R



The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface*  

PubMed Central

The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is “hijacked” by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288,) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 “self”-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N.; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernandez-Robledo, Jose A.; Bianchet, Mario A.; Wang, Lai-Xi; Wilson, Iain B. H.; Vasta, Gerardo R.



Occurrence of metaxenia and false hybrids in Brassica juncea L. cv. Kikarashina x B. napus  

PubMed Central

Imported genetically modified (GM) canola (Brassica napus) is approved by Japanese law. Some GM canola varieties have been found around importation sites, and there is public concern that these may have any harmful effects on related species such as reduction of wild relatives. Because B. juncea is distributed throughout Japan and is known to be high crossability with B. napus, it is assumed to be a recipient of B. napus. However, there are few reports for introgression of cross-combination in B. juncea × B. napus. To assess crossability, we artificially pollinated B. juncea with B. napus. After harvesting a large number of progeny seeds, we observed false hybrids and metaxenia of seed coats. Seed coat color was classified into four categories and false hybrids were confirmed by morphological characteristics and random amplified polymorphic DNA (RAPD) markers. Furthermore, the occurrence of false hybrids was affected by varietal differences in B. napus, whereas that of metaxenia was related to hybridity. Therefore, we suggest that metaxenia can be used as a marker for hybrid identification in B. juncea L. cv. Kikarashina × B. napus. Our results suggest that hybrid productivity in B. juncea × B. napus should not be evaluated by only seed productivity, crossability ought to be assessed the detection of true hybrids. PMID:23136472

Tsuda, Mai; Konagaya, Ken-ichi; Okuzaki, Ayako; Kaneko, Yukio; Tabei, Yutaka



Optimization of Freeze Drying Conditions for Purified Pectinase from Mango (Mangifera indica cv. Chokanan) Peel  

PubMed Central

Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (?2.66, 62.66 mg/mL), Arabic gum (?1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. PMID:22489134

Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd



7-Polyacylated delphinidin 3,7-diglucosides from the blue flowers of Leschenaultia cv. Violet Lena.  


The triacyl anthocyanins, Leschenaultia blue anthocyanins 1 and 2 (LBAs 1 and 2) were isolated from the blue flowers of Leschenaultia R. Br. cv. Violet Lena (Goodeniaceae), in which LBA 1 was present as a dominant pigment. The structure of LBA 1 was elucidated to be delphinidin 3-O-[6-O-(malonyl)-beta-D-glucopyranoside]-7-O-[6-O-(4-O-(6-O-(4-O-(beta-D-glucopyranosyl)-trans-caffeoyl)-beta-D-glucopyranosyl)-trans-caffeoyl)-beta-D-glucopyranoside] by application of chemical and spectroscopic methods. Since LAB 2 was isolated in small amount, its structure was tentatively assigned as either delphinidin 3-(malonylglucoside)-7-[(glucosyl-p-coumaroyl)-(glucosylcaffeoyl)-glucoside] or delphinidin 3-(malonyl-glucoside)-7-[(glucosyl-caffeoyl)(glucosyl-p-coumaroyl)-glucoside]. This is the first report of the occurrence of 7-polyacylated anthocyanins in the family of Goodeniaceae, although others have been found in the families of the Ranunculaceae, Campanulaceae, and Compositae. Moreover, delphinidin 3-glycoside-7-di-(glucosylcaffeoyl)-glucoside has been reported only in the flowers of Platycodon grandiflorum (Campanulaceae). From a chemotaxonomical viewpoint, the Goodeniaceae may be closely related to the Campanulaceae. PMID:17174991

Saito, Norio; Tatsuzawa, Fumi; Yazaki, Yoshikazu; Shigihara, Atsushi; Honda, Toshio



Measurement of heme efflux and heme content in isolated developing chloroplasts. [Cucumis sativus, cv. Sumter  

SciTech Connect

Hemes destined for cytosolic hemoproteins must originate in one of the cellular compartments which have the capacity for heme synthesis, namely the chloroplast or the mitochondria. Since developing chloroplasts from greening cucumber (Cucumis sativus, cv. Sumter) cotyledons are known to contain complete heme and chlorophyll biosynthetic pathways, they were tested for their capacity export hemes. Picomole quantities of heme were measured by reconstitution of the heme with apo-peroxidase and subsequent determination of peroxidase activity. The assay method was sensitive (as little as 0.7 picomole of heme could be detected in a volume of 100 microliters) and was linear with heme concentration. When intact plastids were incubated with apo-peroxidase, a steady-state rate of efflux between 0.12 and 0.45 picomole heme/minute/milligram plastid protein was measured. The efflux rate was not due to plastid breakage and could be enhanced by incubating with the heme precursor, {delta}-aminolevulinic acid. Cold acetone extraction removed 47 {plus minus} 17 picomoles heme/milligram plastid protein from the total b-type heme pool in the chloroplasts (166 {plus minus} 9 picomoles heme/milligram protein, by acid-acetone extraction). The reconstitution technique provided a similar estimate of readily exchangeable heme in the plastid, 37 {plus minus} 8 picomoles heme/milligram protein (or 6 micromolar in the plastids). These values may be indicative of a free heme pool which exists in the chloroplast.

Thomas, J.; Weinstein, J.D. (Clemson Univ., SC (USA))



Promotion of Flowering in Brassica campestris L. cv Ceres by Sucrose  

PubMed Central

Flower initiation of the quantitative long-day plant Brassica campestris cv Ceres was earlier and at a lower final leaf number when sucrose was added to the medium in which plants were grown in sterile culture. The optimal concentration of sucrose was 40 to 80 millimolar. This flower-promoting effect of sucrose was not osmotic, as mannitol, sodium chloride, and polyethylene glycol were not effective at equal osmotic potentials. Seedlings grown heterotrophically after treatment with 4-chloro-5-(dimethylamino)-2-phenyl-3-(2H)-pyridazinone to prevent chlorophyll accumulation were also induced to form flower primordia earlier as the sucrose concentration in the medium was increased up to 80 millimolar. Inclusion of 4 millimolar sodium nitrate in the culture medium of green plants did not reduce the flower-promoting effects of sucrose but delayed initiation in plants grown without added sucrose. Removal of CO2 during a single main or supplementary light period, or both, greatly reduced flower initiation. It is concluded that sucrose may be an important controlling factor determining floral initiation in Brassica. PMID:16663739

Friend, Douglas J. C.; Bodson, Monique; Bernier, Georges



Transpiration, crop coefficient and water use of Olive tree (cv. Cordovil) in Southern Portugal  

NASA Astrophysics Data System (ADS)

Orchard olive transpiration, soil water status and stomatal response in relation to water deficit were investigated to clarify mechanisms of tree water uptake and stomatal control to improve the irrigation scheduling of low-density olive trees of cv. Cordovil grown in typical Mediterranean environment of Southern Portugal. Trees were subject to three irrigation treatments. Treatment A received 100% of crop evapotranspiration by a drip irrigation system, a sustained deficit (SDI)treatment B received 60% of crop evapotranspiration, a regulated deficit(RDI) irrigation treatment C received irrigation water before-flowering and just before pit-hardening, and a Dry-farming treatment. Tree and orchard transpiration and the dynamics of water uptake by roots were estimated from sap flow measurements and water balance technique. Stomatal conductance was modeled from local meteorological variables, measured sap flow and tree canopy variables. Higher than treatment A and B stomatal conductance and the high tree fruit production recommend treatment C as most suitable for scheduling irrigation of olive orchards in wet years of well distributed late summer rainfall. For drier years of no summer and early autumn rains that minimizes available water to extract by roots outside the wet bulb of drip irrigation and for the scarce readily available irrigation water years, as so often occurs in the region, the sustained deficit irrigation (SDI) regime seems a better option. Nonetheless, for years of limited available water resources that preclude sustained deficit irrigation, careful management of the proposed RDI could also allow for efficient use of irrigation water.

Santos, F. L.



Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)  

PubMed Central

A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 ?M, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 ?M. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172

Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng



Using MOST to reveal the secrets of the mischievous Wolf-Rayet binary CV Ser  

E-print Network

The WR binary CV Serpentis (= WR113, WC8d + O8-9IV) has been a source of mystery since it was shown that its atmospheric eclipses change with time over decades, in addition to its sporadic dust production. The first high-precision time-dependent photometric observations obtained with the MOST space telescope in 2009 show two consecutive eclipses over the 29d orbit, with varying depths. A subsequent MOST run in 2010 showed a seemingly asymmetric eclipse profile. In order to help make sense of these observations, parallel optical spectroscopy was obtained from the Mont Megantic Observatory (2009, 2010) and from the Dominion Astrophysical Observatory (2009). Assuming these depth variations are entirely due to electron scattering in a beta-law wind, an unprecedented 62% increase in mass-loss rate is observed over one orbital period. Alternatively, no change in mass-loss rate would be required if a relatively small fraction of the carbon ions in the wind globally recombined and coaggulated to form carbon dust grai...

David-Uraz, Alexandre; Chené, André-Nicolas; Rowe, Jason F; Lange, Nicholas; Guenther, David B; Kuschnig, Rainer; Matthews, Jaymie M; Rucinski, Slavek M; Sasselov, Dimitar; Weiss, Werner W



The BANANA Project. V. Misaligned and Precessing Stellar Rotation Axes in CV Velorum  

NASA Astrophysics Data System (ADS)

As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of ?p = -52° ± 6° and ?s = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i sstarf) over the last 30 yr, leading to a model in which the primary star's obliquity is ?65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i sstarf. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary. Based on observations made with ESOs 2.2 m Telescopes at the La Silla Paranal Observatory under programme ID 084.C-1008 and under MPIA guaranteed time.

Albrecht, Simon; Winn, Joshua N.; Torres, Guillermo; Fabrycky, Daniel C.; Setiawan, Johny; Gillon, Michaël; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter



Visualisation and quantification of CV chondrite petrography using micro-tomography  

NASA Astrophysics Data System (ADS)

Micro-computed tomography is a non-destructive technique that allows the study of 3D meteorite petrography. The technique produces a unique and instructive visualisation of the meteorite for quantifying its components. We studied the overall petrography of the two CV chondrites Allende and Mokoia to constrain their formation histories. A set of movies and stereographic images detail the 3D petrography. Component modal abundances agree with previous reports and modal abundance differences between Allende and Mokoia support the chondrule-matrix complementarity and that chondrules and matrix formed from the same chemical reservoir. We identified two types of chondrules, a normal type and one where a normal type I or II chondrule is almost completely encapsulated by an opaque-rich layer. This layer was probably acquired during a late stage condensation process. The appearance of opaques in chondrules and matrix is different, not supporting a genetic relationships between these. Low abundances of compound chondrules (1.75 vol% in Allende and 2.50 vol% in Mokoia) indicate low chondrule densities and/or low relative component velocities in chondrule formation regions. Porosities on a scale <10-20 ?m allowed for only local aqueous alteration processes on the meteorite parent bodies.

Hezel, Dominik C.; Elangovan, Premkumar; Viehmann, Sebastian; Howard, Lauren; Abel, Richard L.; Armstrong, Robin



Using MOST to reveal the secrets of the mischievous Wolf-Rayet binary CV Ser  

NASA Astrophysics Data System (ADS)

The Wolf-Rayet (WR) binary CV Serpentis (= WR113, WC8d + O8-9IV) has been a source of mystery since it was shown that its atmospheric eclipses change with time over decades, in addition to its sporadic dust production. The first high-precision time-dependent photometric observations obtained with the Microvariability and Oscillations of STars (MOST) space telescope in 2009 show two consecutive eclipses over the 29-d orbit, with varying depths. A subsequent MOST run in 2010 showed a seemingly asymmetric eclipse profile. In order to help make sense of these observations, parallel optical spectroscopy was obtained from the Mont Megantic Observatory (2009, 2010) and from the Dominion Astrophysical Observatory (2009). Assuming these depth variations are entirely due to electron scattering in a ?-law wind, an unprecedented 62 per cent increase in M? is observed over one orbital period. Alternatively, no change in mass-loss rate would be required if a relatively small fraction of the carbon ions in the wind globally recombined and coaggulated to form carbon dust grains. However, it remains a mystery as to how this could occur. There also seems to be evidence for the presence of corotating interaction regions (CIR) in the WR wind: a CIR-like signature is found in the light curves, implying a potential rotation period for the WR star of 1.6 d. Finally, a new circular orbit is derived, along with constraints for the wind collision.

David-Uraz, Alexandre; Moffat, Anthony F. J.; Chené, André-Nicolas; Rowe, Jason F.; Lange, Nicholas; Guenther, David B.; Kuschnig, Rainer; Matthews, Jaymie M.; Rucinski, Slavek M.; Sasselov, Dimitar; Weiss, Werner W.



Brassinosteroid stimulation of hypocotyl elongation and wall relaxation in pakchoi (Brassica chinensis cv Lei-Choi)  

SciTech Connect

Hypocotyl elongation of pakchoi (Brassica chinensis cv Lei-Choi) was stimulated by applying 300 ng of brassinosteroid (2[alpha],3[alpha],22[beta],23[beta]-tetrahydroxy-24[beta]-methyl-B-homo-7-oxa-5[alpha]-cholestan-6-one, BR) in 1 [mu]L of 50% ethanol to the apex of hypocotyls. BR had its greatest effect on elongation of the apical 3-mm region below the cotyledonary node (75% stimulation) between 6 and 18 h after treatment. Stress/strain (Instron) analysis of this 3-mm region revealed that plastic and elastic components of extension were not significantly different between BR-treated and control seedlings. In pressure-block experiments, the initial rate of relaxation was 2-fold faster in BR-treated plants as compared with controls, whereas after 125 min the total amount of relaxation and the relaxation rate were the same for the two treatments. Osmotic pressure of cell sap expressed from this 3-mm region showed a large decrease (28%) in BR-treated seedlings compared to the controls. The authors conclude that BR stimulates growth in pakchoi by accelerating the biochemical processes that cause wall relaxation, without inducing a large change in wall mechanical properties. 19 refs., 3 figs., 1 tab.

Tzannwei Wang; Cosgrove, D.J.; Arteca, R.N. (Pennsylvania State Univ., University Park (United States))



Diurnal cycles of embolism formation and repair in petioles of grapevine (Vitis vinifera cv. Chasselas)  

PubMed Central

The impact of water deficit on stomatal conductance (gs), petiole hydraulic conductance (Kpetiole), and vulnerability to cavitation (PLC, percentage loss of hydraulic conductivity) in leaf petioles has been observed on field-grown vines (Vitis vinifera L. cv. Chasselas). Petioles were highly vulnerable to cavitation, with a 50% loss of hydraulic conductivity at a stem xylem water potential (?x) of –0.95?MPa, and up to 90% loss of conductivity at a ?x of –1.5?MPa. Kpetiole described a daily cycle, decreasing during the day as water stress and evapotranspiration increased, then rising again in the early evening up to the previous morning's Kpetiole levels. In water-stressed vines, PLC increased sharply during the daytime and reached maximum values (70–90%) in the middle of the afternoon. Embolism repair occurred in petioles from the end of the day through the night. Indeed, PLC decreased in darkness in water-stressed vines. PLC variation in irrigated plants showed the same tendency, but with a smaller amplitude. The Chasselas cultivar appears to develop hydraulic segmentation, in which petiole cavitation plays an important role as a ‘hydraulic fuse’, thereby limiting leaf transpiration and the propagation of embolism and preserving the integrity of other organs (shoots and roots) during water stress. In the present study, progressive stomatal closure responded to a decrease in Kpetiole and an increase in cavitation events. Almost total closure of stomata (90%) was measured when PLC in petioles reached >90%. PMID:21447755

Zufferey, V.; Cochard, H.; Ameglio, T.; Spring, J.-L.; Viret, O.



Air-sampling inlet contamination by aircraft emissions on the NASA CV-990 aircraft  

NASA Technical Reports Server (NTRS)

Results of an experimental investigation of the contamination of air sampling inlets by aircraft emissions from the NASA CV-990 research aircraft are presented. This four-engine jet aircraft is a NASA facility used for many different atmospheric and meteorological experiments, as well as for developing spacecraft instrumentation for remote measurements. Our investigations were performed to provide information on which to base the selection of sampling locations for a series of multi-instrument missions for measuring tropospheric trace gases. The major source of contamination is the exhaust from the jet engines, which generate many of the same gases that are of interest in atmospheric chemistry, as well as other gases that may interfere with sampling measurements. The engine exhaust contains these gases in mixing ratios many orders of magnitude greater than those that occur in the clean atmosphere which the missions seek to quantify. Pressurized samples of air were collected simultaneously from a scoop located forward of the engines to represent clean air and from other multiport scoops at various aft positions on the aircraft. The air samples were analyzed in the laboratory by gas chromatography for carbon monoxide, an abundant combustion by-product. Data are presented for various scoop locations under various flight conditions.

Condon, E. P.; Vedder, J. F.



CV-990 Landing Systems Research Aircraft (LSRA) flight #145 drilling of shuttle tire using Tire Assa  

NASA Technical Reports Server (NTRS)

Created from a 1/16th model of a German World War II tank, the TAV (Tire Assault Vehicle) was an important safety feature for the Convair 990 Landing System Research Aircraft, which tested space shuttle tires. It was imperative to know the extreme conditions the shuttle tires could tolerate at landing without putting the shuttle and its crew at risk. In addition, the CV990 was able to land repeatedly to test the tires. The TAV was built from a kit and modified into a radio controlled, video-equipped machine to drill holes in aircraft test tires that were in imminent danger of exploding because of one or more conditions: high air pressure, high temperatures, and cord wear. An exploding test tire releases energy equivalent to two and one-half sticks of dynamite and can cause severe injuries to anyone within 50 ft. of the explosion, as well as ear injury - possibly permanent hearing loss - to anyone within 100 ft. The degree of danger is also determined by the temperature pressure and cord wear of a test tire. The TAV was developed by David Carrott, a PRC employee under contract to NASA.



[Changes in polyamine levels in Citrus sinensis Osb. cv. Valencia callus during somatic embryogenesis].  


Somatic embryogenetic capability and changes in polyamine level and their relationship were analyzed using the long-term (8 years) subcultured calli of Citrus sinensis Osb. cv. Valencia as materials. The results showed that endogenous polyamine contents in embryogenic calli were higher than those in non-embryogenic calli, and the embryogenetic capability was positively correlated to the levels of endogenous polyamines. When the calli were transferred to a differentiation medium, the putrescine content rapidly increased and reached a peak, then fell gradually. Applying exogenous putrescine raised the embryogenesis frequency and endogenous putrescine level. It indicated that increase in putrescine content at early stage of differentiation promoted embryogenesis. With the development of somatic embryo, spermidine content reached its the highest level at globular embryo stage, spermine content rose and reached a peak at a later stage of globular embryo development. Furthermore, changes of the putrescine, spermidine and spermine contents during somatic embryogenesis were similar in Valencia calli which had different ploidy levels, but their contents decreased following the increasing of ploidy level. Changes in arginine decarboxylase activity were positively correlated to the polyamine levels, which suggest that the later is a key factor in regulating the polyamine levels during somatic embryogenesis in citrus plants. PMID:15961902

Liu, Hua-Ying; Xiao, Lang-Tao; Lu, Xu-Dong; Hu, Jia-Jin; Wu, Shun; He, Chang-Zheng; Deng, Xiu-Xin



Effect of Gibberellin and Auxin on Parthenocarpic Fruit Growth Induction in the cv Micro-Tom of Tomato  

Microsoft Academic Search

The effect of applied gibberellin (GA) and auxin on fruit-set and growth has been investigated in tomato (Solanum lycopersicum L.) cv Micro-Tom. It was found that to prevent competition between developing fruits only one fruit per truss should be left\\u000a on the plant. Unpollinated ovaries responded to GA3 and to different auxins [indol-3-acetic acid, naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid (2,4-D)],

Juan C. Serrani; Mariano Fos; Alejandro Atarés; José L. García-Martínez



Improved Antioxidant and Anti-inflammatory Potential in Mice Consuming Sour Cherry Juice ( Prunus Cerasus cv. Maraska)  

Microsoft Academic Search

The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and\\u000a anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and\\u000a brain), LPO (liver, brain)

Ana Šari?; Sandra Sobo?anec; Tihomir Balog; Borka Kuši?; Višnja Šverko; Verica Dragovi?-Uzelac; Branka Levaj; Zrinka ?osi?; Željka Ma?ak Šafranko; Tatjana Marotti



The influence of storage on aroma, soluble solids, acid and colour of sour cherries (Prunus cerasus L.) cv. Stevnsbær  

Microsoft Academic Search

The present study involved a laboratory scale experiment where the impact of post-harvest storage on the quality of sour\\u000a cherries (Prunus cerasus L.) cv Stevnsb?r was investigated. Cherries were stored for 7 days at temperatures of 2???C, 10???C, 20???C, and 30???C,\\u000a and at 20???C in combination with a 20% CO2 atmosphere. Cherry quality was assessed by analysis of soluble solids,

M. B. Petersen; L. Poll



Effect of different brining treatments on the fermentation of cv. Conservolea green olives processed by the Spanish-method  

Microsoft Academic Search

The effect of different initial brining conditions on the spontaneous fermentation of cv. Conservolea green olives was studied. The different treatments included: (a) brine acidification with 2‰ (v\\/v) lactic acid (control), (b) addition of 25‰ (v\\/v) 1n HCl (c) substitution of the initial brine by 20% (v\\/v) with a brine from a previous fermentation (brine re-use). Microbial growth, pH, titratable

Efstathios Z. Panagou; Constantinos Z. Katsaboxakis



The role of sucrose and different cytokinins in the in vitro floral morphogenesis of rose (hybrid tea) cv. “First Prize”  

Microsoft Academic Search

Shoots of rose (hybrid tea) cv. “First Prize” were induced to flower in vitro on Murashige and Skoog (MS) medium containing various sucrose concentrations (15, 30 or 45 g l?1) and different phytohormone combinations of different cytokinins [N6-benzyladenine (BA); thidiazuron (TDZ) and zeatin] with ?-naphthaleneacetic acid (NAA). Results indicate that sucrose is the key factor in floral morphogenesis while cytokinin increases the

Nguyen Hong Vu; Phan Hoang Anh; Duong Tan Nhut



Plant Regeneration from Immature Inflorescence Culture and Genetic Transformation of Wheatgrass ( Agropyron cristatum × A. desertorum cv. Hycrest-Mengnong)  

Microsoft Academic Search

The plants of hybrid wheatgrass (A. cristatum × A. desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on improved MS medium containing 2.0-3.0 mg L?1 2,4-D to regenerate callus. The calli were then transferred to hormone-free MS medium for differentiation and 1\\/2 MS medium for rooting. Results showed that callus initiation

Xiu-wen HUO; Jian-hua WEI; Chun-bo XU; Fu-gui MI; Jin-feng YUN



Transformation of triploid bermudagrass ( Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment  

Microsoft Academic Search

A transformation system for triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 wM dicamba, 20 wM benzylaminopurine, and 100 mg\\/l myo-inositol. Using the hygromycin phosphotransferase (hpt) gene as the selectable marker gene, we obtained 75

G. Zhang; S. Lu; T. A. Chen; C. R. Funk; W. A. Meyer



Specific disruption of vimentin filament organization in monkey kidney CV-1 cells by diphtheria toxin, exotoxin A, and cycloheximide.  

PubMed Central

We have examined the effect of diphtheria toxin, Pseudomonas aeruginosa exotoxin A, and cycloheximide on the CV-1 cell cytoskeleton. Within a few hours after producing an inhibition of cellular protein synthesis, all these agents specifically disrupted the organization of the vimentin filament system with no discernable effect on microtubules or microfilaments during the period of observation. Furthermore, just as the inhibition of protein synthesis by cycloheximide is reversible, so was the disruption of vimentin filaments by cycloheximide. Images PMID:6784120

Sharpe, A H; Chen, L B; Murphy, J R; Fields, B N



Pathogenicity and reproductive fitness of Pratylenchus coffeae and Radopholus arabocoffeae on Arabica coffee seedlings ( Coffea arabica cv. Catimor) in Vietnam  

Microsoft Academic Search

The pathogenicity and reproductive fitness of Pratylenchus coffeae and Radopholus arabocoffeae from Vietnam on coffee (Coffea arabica) seedlings cv. Catimor were evaluated in greenhouse experiments. The effect of initial population densities (Pi?=?0, 1, 2,\\u000a 4, 8, 16, 32, 64, 128, and 256 nematodes per cm3 soil) was studied for both species at different days after inoculation (dai). The data were

Phap Q. Trinh; Wim M. L. Wesemael; Sy T. T. Nguyen; Chau N. Nguyen; Maurice Moens



OpenCV C# wrapper based video enhancement using different optical flow methods in the super-resolution  

Microsoft Academic Search

This article presents a simple method on how to implement a super-resolution based video enhancement technique in .NET using the functions of the OpenCV library. First, we outline the goal of this project and after that, a short review of the steps of super-resolution technique is given. As a part of the discussion about the concrete implementation, firstly the general

A. Nagy; Z. Vamossy



In vitro production and chemical characterization of St. John’s wort ( Hypericum perforatum L. cv ‘New Stem’)  

Microsoft Academic Search

Hypericum perforatum L. (St. John’s wort) is a traditional medicinal plant that has been used for the treatment of neurological disorders and depression. To investigate a large-scale in vitro growth system for St. John’s wort cv ‘New Stem’, six different culture systems, balloon type bubble bioreactor, temporary immersion bioreactor, temporary root zone immersion bioreactor, Erlenmeyer flask, large vessel with gelled

S. M. A. Zobayed; S. J. Murch; H. P. V. Rupasinghe; P. K. Saxena



????? gibberellic acid ???????????????? ???????????????????????????? Effect of Gibberellic Acid on Fruit Quality of Dragon Fruit (Hylocereus undatus) cv. While aril  

Microsoft Academic Search

Five-year old dragon fruit trees cv. Vietnam were treated with GA3 by spraying at 0,10,20 ppm 1 day before blooming and 7 days after blooming. The experiment was conducted at a private orchard in Warin Chamrap district, Ubon Ratchathani province during the period from January to October 2005. The results showed a significant difference in length of fruits, weight of

Ratchadaporn Janthasri; Umaporn Rajun; Satin Pasuvitayakun


[Leaf anatomy of the mosaic ficus benjamina cv. Starlight and interaction of source and sink chimera components].  


Leaf anatomy was studied in the mosaic Ficus benjamina cv. Starlight and non-chimeric Ficus benjamina cv. Daniel. The number of chloroplasts in a white, chlorophyll-deficient tissue declines as compared to the green tissue. However, their functional activity is retained. The leaf of the mosaic F. benjamina contains two or, sometimes, three subepidermal layers. Mesophyll forms one layer in the green and white parts of leaf palisade and one white and one green layer in the transitional zone (edge). In the transitional zone, green spongy mesophyll is located between two white spongy layers and the proportion of photosynthesizing cells varies. In cv. Daniel, there are two subepidermal layers and one layer of columnar mesophyll cells. According to the morphometry data, the proportion of white zone in the leaf correlates with the leaf position in the whole shoot: the higher the branch order, the larger the proportion of white zone. The total leaf area depends also on its position in the shoot. No such correlation was found in non-chimeric F. benjamina cv. Daniel. In the mosaic chimera, the source-sink status appears to depend on the leaf position in the shoot. Experiments with individual shoots of the same order and elimination of all lateral shoots have shown that the proportion of white zone in new leaves on the shoot increases with the total area of green zone. Thus, the area of assimilating shoot surface affects the formation of leaves in the meristem. A hypothesis was put forward that the source-sink state affects the ratio of green and white parts in the leaf primordium. Products of photosynthesis (carbohydrates) are a possible metabolic signal affecting the meristem. It cannot be excluded as well that the hormonal state undergoes changes in the chimeric plant. PMID:18179027

Labunskaia, E A; Zhigalova, T V; Chub, V V



S.stapfianus and E. curvula cv. Consol in vivo photosynthesis, PSII activity and ABA content during dehydration  

Microsoft Academic Search

Changes in photosynthetic activity, CO2 assimilation rate, FPSII by fluorescence andABA content, were monitored in the grasses Eragrostis curvula cv. Consol and Sporobolusstapfianus Gandoger in response to dehydration. Thefirst being a warm season grass well adapted todrought and the second a desiccation-tolerant orresurrection plant. The trial was performed on intactleaves during a whole plant drying course. After acycle of dehydration

S. Di Blasi; S. Puliga; L. Losi; C. Vazzana



Combined in situ EC-AFM and CV measurement study on lead electrode for lead–acid batteries  

Microsoft Academic Search

An electrochemical atomic force microscope (EC-AFM) was used to study the reaction of a lead electrode in sulfuric acid electrolyte, when the reaction corresponding to what occurs at the negative electrode of a lead–acid battery took place. At first, the AFM was applied to observation of the lead electrode during cyclic-voltammetry (CV) measurement, and was found to be useful to

Yoshiaki Yamaguchi; Masashi Shiota; Yasuhide Nakayama; Nobumitsu Hirai; Shigeta Hara



Anaerobic n-Alkane Metabolism by a Sulfate-Reducing Bacterium, Desulfatibacillum aliphaticivorans Strain CV2803T  

Microsoft Academic Search

The alkane-degrading, sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T, recently isolated from marine sediments, was investigated for n-alkane metabolism. The total cellular fatty acids of this strain had predominantly odd numbers of carbon atoms (C odd) when the strain was grown on a C-odd alkane (pentadecane) and even numbers of carbon atoms (C even) when it was grown on a C-even

Cristiana Cravo-Laureau; Vincent Grossi; Danielle Raphel; Robert Matheron; Agnes Hirschler-Rea



Improved antioxidant and anti-inflammatory potential in mice consuming sour cherry juice (Prunus Cerasus cv. Maraska).  


The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties. PMID:19763832

Sari?, Ana; Sobocanec, Sandra; Balog, Tihomir; Kusi?, Borka; Sverko, Visnja; Dragovi?-Uzelac, Verica; Levaj, Branka; Cosi?, Zrinka; Macak Safranko, Zeljka; Marotti, Tatjana



Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation  

PubMed Central

Background Ultraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV-B treatment. Some reports have analyzed the changes in gene expression caused by UV-B light on several plant species using microarray technology. However, there is no information on the transcriptome response triggered by UV-B in grapevine. In this paper we investigate the gene expression responses of leaves from in vitro cultured Vitis vinifera cv. Malbec plants subjected to the same dose of biologically effective UV-B radiation (4.75 kJ m-2 d-1) administered at two different fluence rates (16 h at ? 8.25 ?W cm-2, 4 h at ? 33 ?W cm-2) using a new custom made GrapeGen Affymetrix GeneChip®. Results The number of genes modulated by high fluence rate UV-B doubled the number of genes modulated by low fluence UV-B. Their functional analyses revealed several functional categories commonly regulated by both UV-B treatments as well as categories more specifically modulated depending on UV-B fluence rate. General protective responses, namely the induction of pathways regulating synthesis of UV-B absorbing compounds such as the Phenylpropanoid pathway, the induction of different antioxidant defense systems and the activation of pathways commonly associated with pathogen defense and abiotic stress responses seem to play critical roles in grapevine responses against UV-B radiation. Furthermore, high fluence rate UV-B seemed to specifically modulate additional pathways and processes in order to protect grapevine plantlets against UV-B-induced oxidative stress, stop the cell cycle progression, and control protein degradation. On the other hand, low fluence rate UV-B regulated the expression of specific responses in the metabolism of auxin and abscisic acid as well as in the modification of cell walls that could be involved in UV-B acclimation-like processes. Conclusion Our results show the UV-B radiation effects on the leaf transcriptome of grapevine (Vitis vinifera cv. Malbec) plantlets. Functional categories commonly modulated under both UV-B treatments as well as transcripts specifically regulated in an UV-B-intensity dependent way were identified. While high fluence rate UV-B had regulatory effects mainly on defense or general multiple-stress responses pathways, low fluence rate UV-B promoted the expression of genes that could be involved in UV-B protection or the amelioration of the UV-B-induced damage. This study also provides an extensive list of genes regulating multiple metabolic pathways involved in the response of grapevine to UV-B that can be used for future researches. PMID:20959019



Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston 1  

PubMed Central

Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived (MD) embryo culture system. TAGs accumulating during embryo development exhibited changes in acyl composition similar to those observed in developing zygotic embryos of the same cv, particularly with respect to erucic and eicosenoic acids. However, MD embryos showed a much higher rate of incorporation of 14C-erucoyl moieties into TAGs in vitro than zygotic embryos. Homogenates of early-late cotyledonary stage MD embryos (14-29 days in culture) were assessed for the ability to incorporate 22:1 and 18:1 (oleoyl) moieties into glycerolipids. In the presence of [1-14C]22:1-coenzyme A (CoA) and various acyl acceptors, including glycerol-3-phosphate (G-3-P), radiolabeled erucoyl moieties were rapidly incorporated into the TAG fraction, but virtually excluded from other Kennedy Pathway intermediates as well as complex polar lipids. This pattern of erucoyl incorporation was unchanged during time course experiments or upon incubation of homogenates with chemicals known to inhibit Kennedy Pathway enzymes. In marked contrast, parallel experiments conducted using [1-14C]18:1-CoA and G-3-P indicated that 14C oleoyl moieties were incorporated into lyso-phosphatidic acids, phosphatidic acids, diacylglycerols, and TAGs of the Kennedy Pathway, as well as other complex polar lipids, such as phosphatidylcholines and phosphatidylethanolamines. When supplied with l-[2-3H(N)]G-3-P and [1-14C]22:1-CoA, the radiolabeled TAG pool contained both isotopes, indicating G-3-P to be a true acceptor of erucoyl moieties. Radio-high-performance liquid chromatography, argentation thin-layer chromatography/gas chromatography-mass spectrometry, and stereospecific analyses of radiolabeled TAGs indicated that 22:1 was selectively incorporated into the sn-3 position by a highly active diacylglycerol acyltransferase (DGAT; EC, while oleoyl moieties were inserted into the sn-1 and sn-2 positions. In the presence of sn-1,2-dierucin and [1-14C]22:1-CoA, homogenates and microsomal preparations were able to produce radiolabeled trierucin, a TAG not found endogenously in this species. A 105,000g pellet fraction contained 22:1-CoA:DGAT exhibiting the highest specific activity. The rate of 22:1-CoA:DGAT activity in vitro could more than account for the maximal rate of TAG biosynthesis observed in vivo during embryo development. In double label experiments, G-3-P was shown to stimulate the conversion of [3H]phosphatidylcholines to [3H]diacylglycerols, which subsequently acted as acceptors for 14C erucoyl moieties. In vitro, 22:1 moieties did not enter the sn-1 position of TAGs by a postsynthetic modification or transacylation of preformed TAGs. PMID:16668417

Taylor, David C.; Weber, Nikolaus; Barton, Dennis L.; Underhill, Edward W.; Hogge, Lawrence R.; Weselake, Randall J.; Pomeroy, M. Keith



Structural and functional characterization of proteinase inhibitors from seeds of Cajanus cajan (cv. ICP 7118).  


Proteinase inhibitors (C11PI) from mature dry seeds of Cajanus cajan (cv. ICP 7118) were purified by chromatography which resulted in 87-fold purification and 7.9% yield. SDS-PAGE, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF/TOF) mass spectrum and two-dimensional (2-D) gel electrophoresis together resolved that C11PI possessed molecular mass of 8385.682 Da and existed as isoinhibitors. However, several of these isoinhibitors exhibited self association tendency to form small oligomers. All the isoinhibitors resolved in Native-PAGE and 2-D gel electrophoresis showed inhibitory activity against bovine pancreatic trypsin and chymotrypsin as well as Achaea janata midgut trypsin-like proteases (AjPs), a devastating pest of castor plant. Partial sequences of isoinhibitor (pI 6.0) obtained from MALDI-TOF/TOF analysis and N-terminal sequencing showed 100% homology to Bowman-Birk Inhibitors (BBIs) of leguminous plants. C11PI showed non-competitive inhibition against trypsin and chymotrypsin. A marginal loss (<15%) in C11PI activity against trypsin at 80 (°)C and basic pH (12.0) was associated with concurrent changes in its far-UV CD spectra. Further, in vitro assays demonstrated that C11PI possessed significant inhibitory potential (IC50 of 78 ng) against AjPs. On the other hand, in vivo leaf coating assays demonstrated that C11PI caused significant mortality rate with concomitant reduction in body weight of both larvae and pupae, prolonged the duration of transition from larva to pupa along with formation of abnormal larval-pupal and pupal-adult intermediates. Being smaller peptides, it is possible to express C11PI in castor to protect them against its devastating pest A. janata. PMID:25093261

Swathi, Marri; Lokya, Vadthya; Swaroop, Vanka; Mallikarjuna, Nalini; Kannan, Monica; Dutta-Gupta, Aparna; Padmasree, Kollipara



Establishment of regeneration and transformation system in Egyptian sesame (Sesamum indicum L.) cv Sohag 1.  


Sesame (Sesamum indicum L.) is an important oil crop in many tropical and sub-tropical regions of the world, yet has received little attention in applying modern biotechnology in its improvement due to regeneration and transformation difficulties. Here within, we report the successful production of transgenic fertile plants of sesame (cv Sohag 1), after screening several cultivars. Agrobacterium tumefaciens- carrying the pBI121 plasmid {neomycin phosphotransferase gene (NPTII) and a ?-glucuronidase gene (GUS)} was used in all experiments. Recovery of transgenic sesame shoots was achieved using shoot induction medium (Murashige and Skoog MS basal salt mixture + Gamborg's B5 vitamins + 2.0 mg/l BA + 1.0 mg/l IAA + 5.0 mg/l AgNO3 + 30.0 g/l sucrose + 7.0 g/l agar + 200 mg/l cefotaxime and 25 mg/l kanamycin) and shoots were rooted on MS medium + B5 vitamins + 1.0 mg/l IAA + 10.0 g/l sucrose and 7.0 g/l agar. Rooted shoots were transplanted into soil and grown to maturity in greenhouse. Incorporation and expression of the GUS gene into T0 sesame plants was confirmed using polymerase chain reaction (PCR), reverse transcriptase-PCR (RT-PCR) and GUS histochemical assay. Several factors were found to be important for regeneration and transformation in sesame. The most effective were plant genotype and the addition of AgNO3 for successful recovery of sesame shoots. Co-cultivation time and optical density of the Agrobacterium were also critical for sesame transformation. This work is an attempt to open the door for further genetic improvement of sesame using important agronomic traits. PMID:22179191

Al-Shafeay, Amal F; Ibrahim, Ahmed S; Nesiem, Mohamed R; Tawfik, Mohamed S



Time-resolved observations of the short period CV SDSS J123813.73-033933.0  

E-print Network

We observed a new and poorly studied cataclysmic variable (CV) SDSS J123813.73-033933.0 to determine its classification and binary parameters. Simultaneous time-resolved photometric and spectroscopic observations were carried out to conduct period analysis and Doppler tomography mapping. From radial velocity measurements of the Ha line we determined its orbital period to be 0.05592+/-0.00035$ days (80.53min). This period is longer than the first estimate of 76 min by Szkody et al. (2003), but still at the very edge of the period limit for hydrogen-rich CVs. The spectrum shows double-peaked Balmer emission lines flanked by strong broad Balmer absorption, indicating a dominant contribution by the white dwarf primary star, and is similar to the spectra of short-period low-mass transfer WZ Sge-like systems. The photometric light curve shows complex variability. The system undergoes cyclic brightening up to 0.4 mags, which are of semi-periodic nature with periods of the order of 8-12 hours. We also detect a 40.25 min variability of ~0.15 mag corresponding to half of the orbital period. Amplitude of the latter increases with the cyclic brightening of the system. We discuss the variable accretion rate and its impact on the hot spot as the most probable reason for both observed processes. SDSS J123813.73-033933.0 is preliminary classified as a WZ Sge-like short period system with low and unstable accretion rate.

S. V. Zharikov; G. H. Tovmassian; R. Napiwotzki; R. Michel; V. Neustroev



Effect of quarantine treatments on the carbohydrate and organic acid content of mangoes (cv. Tommy Atkins)  

NASA Astrophysics Data System (ADS)

Brazil is one of the largest mango producers and the third largest mango exporter worldwide. Irradiation treatment and its commercial feasibility have been studied in our country to make it possible to develop new markets and, consequently, to compete with the major exporters of mangoes, Mexico and India. This work was designed to compare irradiation treatment with the hot water dip treatment in mangoes cv. Tommy Atkins for export and to verify that the main attributes for acceptance, color and texture, as well as carbohydrate and organic acid contents, were maintained. In this study, the fruit was divided into groups: control, hot water dip-treated (46 °C for 90 min), and irradiation-treated at doses of 0.4 kGy and 1.0 kGy. The fruit was stored at low temperature (11 °C±2) for 14 days and then at room temperature (23 °C±2) until the end of the study. The results indicated that the fruit given a dose of 1.0 kGy remained in a less advanced stage of ripening (stage 3) throughout the storage period, but experienced a greater loss of texture in the beginning of the experiment. It was noted that only the control group had higher levels of citric acid and succinic acid on the last day of the experiment. There were no significant differences in the total sugar content between any treatment groups. Gamma radiation can be used as a quarantine treatment and does not interfere negatively with the quality attributes of mangoes.

Cruz, J. N.; Soares, C. A.; Fabbri, A. D. T.; Cordenunsi, B. R.; Sabato, S. F.



Biochemical properties of alpha-amylase from peel of Citrus sinensis cv. Abosora.  


alpha-Amylase activity was screened in the peel, as waste fruit, of 13 species and cultivars of Egyptian citrus. The species Citrus sinensis cv. Abosora had the highest activity. alpha-Amylase AI from Abosora peel was purified to homogeneity using anion and cation-exchange, and gel filtration chromatographies. Molecular weight of alpha-amylase AI was found to be 42 kDa. The hydrolysis properties of alpha-amylase AI toward different substrates indicated that corn starch is the best substrate. The alpha-amylase had the highest activity toward glycogen compared with amylopectin and dextrin. Potato starch had low affinity toward alpha-amylase AI but it did not hydrolyze beta-cyclodextrin and dextran. Apparent Km for alpha-amylase AI was 5 mg (0.5%) starch/ml. alpha-Amylase AI showed optimum activity at pH 5.6 and 40 degrees C. The enzyme was thermally stable up to 40 degrees C and inactivated at 70 degrees C. The effect of mono and divalent metal ions were tested for the alpha-amylase AI. Ba2+ was found to have activating effect, where as Li+ had negligible effect on activity. The other metals caused inhibition effect. Activity of the alpha-amylase AI was increased one and half in the presence of 4 mM Ca2+ and was found to be partially inactivated at 10 mM Ca2+. The reduction of starch viscosity indicated that the enzyme is endoamylase. The results suggested that, in addition to citrus peel is a rich source of pectins and flavanoids, alpha-amylase AI from orange peel could be involved in the development and ripening of citrus fruit and may be used for juice processing. PMID:19941088

Mohamed, Saleh Ahmed; Drees, Ehab A; El-Badry, Mohamed O; Fahmy, Afaf S



Nitrogen supply affects anthocyanin biosynthetic and regulatory genes in grapevine cv. Cabernet-Sauvignon berries.  


Accumulation of anthocyanins in grape berries is influenced by environmental factors (such as temperature and light) and supply of nutrients, i.e., fluxes of carbon and nitrogen feeding the berry cells. It is established that low nitrogen supply stimulates anthocyanin production in berry skin cells of red varieties. The present works aims to gain a better understanding of the molecular mechanisms involved in the response of anthocyanin accumulation to nitrogen supply in berries from field grown-plants. To this end, we developed an integrated approach combining monitoring of plant nitrogen status, metabolite measurements and transcript analysis. Grapevines (cv. Cabernet-Sauvignon) were cultivated in a vineyard with three nitrogen fertilization levels (0, 60 and 120 kg ha(-1) of nitrogen applied on the soil). Anthocyanin profiles were analyzed and compared with gene expression levels. Low nitrogen supply caused a significant increase in anthocyanin levels at two ripening stages (26 days post-véraison and maturity). Delphinidin and petunidin derivatives were the most affected compounds. Transcript levels of both structural and regulatory genes involved in anthocyanin synthesis confirmed the stimulation of the phenylpropanoid pathway. Genes encoding phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavonoid-3',5'-hydroxylase (F3'5'H), dihydroflavonol-4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) exhibited higher transcript levels in berries from plant cultivated without nitrogen compared to the ones cultivated with 120 kg ha(-1) nitrogen fertilization. The results indicate that nitrogen controls a coordinated regulation of both positive (MYB transcription factors) and negative (LBD proteins) regulators of the flavonoid pathway in grapevine. PMID:24735825

Soubeyrand, Eric; Basteau, Cyril; Hilbert, Ghislaine; van Leeuwen, Cornelis; Delrot, Serge; Gomès, Eric



Nature and origin of interstellar diamond from the Allende CV3 meteorite  

NASA Astrophysics Data System (ADS)

Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende CV3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on 3 mm EM grids. The AEM was performed on transmission-thin fragments of the material which overlay holes in the film, to eliminate interference from the substrate. Electron Spectroscopy for Chemical Analysis (ESCA) was performed on a large aliquot of C. Diamond was identified by selected area electron diffraction. Scanning Transmission Electron Microscope / Energy Dispersive X-ray (STEM-EDS) microanalyses of the C delta diamond, using a light-element detector, show that oxygen and possibly nitrogen are the only impurities consistently present. ESCA spectra from bulk C delta material confirm the presence of N at a level of 0.35 percent or less. Under UV irradiation a yellow-red fluorescence is observed, consistent with that of natural diamonds containing substitutional N. Electron Energy Loss Spectra (EELS) were recorded at 2 eV resolution from the C delta diamond, high pressure synthetic diamond, a diamond film produced in a low pressure plasma by chemical vapor deposition (CVD) on a heated silicon substrate (Roy, 1987), graphite, and amorphous arc sputtered carbon. Comparison of the carbon K edge shape and fine structure shows the Allende C delta phase to be largely diamond, but with a significant pre-edge absorption feature indicative of transitions of C 1s electrons into pi asterisk orbitals which are absent in the purely sp(3)-bonded diamond but present in graphite and amorphous carbon.

Blake, David; Freund, Friedemann; Bunch, Ted; Krishnan, Kannan; Stampfer, Mitch; Chang, Sherwood; Tielens, Alexander G. G. M.



The toxic effects of mature flatpea (Lathyrus sylvestris L cv Lathco) on sheep.  


The toxic effects of mature, seed-bearing flatpea (Lathyrus sylvestris L cv Lathco) hay on sheep was studied in a feeding trial with 25 adult ewes. Five ewes were barren; 20 were in the last 10 w of pregnancy. The ewes were blocked by weight and reproductive status for assignment to treatment groups. Four groups of 5 ewes each were allotted to 1 of the following diets: 0% (controls), 35.0%, 52.5% or 70.0% flatpea (FP); and 1 group of 5 ewes was allotted to a diet of increasing levels of 17.5, 35.0, 52.5 and 70.0% FP. Flatpea hay was harvested in the mature, seed-bearing stage, pelleted, reground and incorporated into a basal (control) diet of 30% corn + 70% alfalfa by substitution of an appropriate amount of FP for alfalfa. The resulting diets were fed for up to 42 days. The toxic nature of FP was expressed by the following dose-related responses: Fluctuating reductions in feed intake upon initiation of diets containing 35% FP and above; occurrence of a potentially fatal, acute central nervous system syndrome at FP levels as low as 35%; and reduced weight gain at FP levels of 52.5% and above. A wasting condition was observed in 1 ewe fed 52.5% FP. Four of 5 ewes and 1 lamb fed increasing levels of FP developed acute poisoning. One of these ewes developed acute neurotoxicosis when consuming 52.5% FP; 3 other ewes and 1 40-d-old orphaned lamb developed neurotoxicosis upon raising the FP level to 70.0%.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:8470354

Rowe, L D; Ivie, G W; DeLoach, J R; Foster, J G



Onco-neural antibodies and tumour type determine survival and neurological symptoms in paraneoplastic neurological syndromes with Hu or CV2/CRMP5 antibodies  

PubMed Central

Objective Anti-Hu antibodies (Hu-Ab) and anti-CV2/CRMP5 antibodies (CV2/CRMP5-Ab) have been identified in association with paraneoplastic neurological disorders. However, it is not clear whether these antibodies are associated with specific neurological symptoms or are only markers of anti-cancer immune reaction. Methods To address this question, we compared 37 patients with CV2/CRMP5-Ab and 324 patients with Hu-Ab. Results Whereas the age and sex ratio were the same between the two groups, the distribution of neurological symptoms was not. Patients with CV2/CRMP5-Ab presented more frequently cerebellar ataxia, chorea, uveo/retinal symptoms and myasthenic syndrome (Lambert-Eaton myasthenic syndrome LEMS or myasthenia gravis). They also had better Rankin score. On the opposite, dysautonomia, brainstem encephalitis and peripheral neuropathy were more frequent in patients with Hu-Ab. Limbic encephalitis occurred similarly in both groups. Small cell lung cancer (SCLC) was the most frequently associated tumor in both groups of patients while malignant thymoma was observed only in patients with CV2/CRMP5-Ab. In particular, patients with CV2/CRMP5-Ab and thymoma developed more frequently myasthenic syndrome while patients with SCLC developed more frequently neuropathies. Chorea and myasthenic syndrome were only seen in patients with CV2/CRMP5-Ab. The median survival time was significantly longer in patients with CV2/CRMP5-Ab and this effect was not dependent on the type of tumor. Interpretation Our data demonstrate that in patients with paraneoplastic neurological syndromes, the neurological symptoms and survival vary with both the type of associated onco-neural antibody and the type of tumor. PMID:18931014

Honnorat, Jerome; Cartalat-Carel, Stephanie; Ricard, Damien; Camdessanche, Jean-Philippe; Carpentier, Antoine F.; Rogemond, Veronique; Chapuis, Francois; Aguera, Michele; Decullier, Evelyne; Duchemin, Anne-Marie; Graus, Francesc; Antoine, Jean-Christophe



CDMRP (eReceipt) PI Guide to Funding G31cv1/ML/CDMRP PI Guide November 2009 Page 1 of 14  

E-print Network

CDMRP (eReceipt) PI Guide to Funding G31cv1/ML/CDMRP PI Guide November 2009 Page 1 of 14 INTERNAL System (eReceipt) A Quick Guide for Applicants applying to the CDMRP for funding CDMRP web page: #12;CDMRP (eReceipt) PI Guide to Funding G31cv1/ML/CDMRP PI Guide November 2009 Page 2 of 14 Contents


Growth and cellular fatty-acid composition of a sulphate-reducing bacterium, Desulfatibacillum aliphaticivorans strain CV2803 T, grown on n-alkenes  

Microsoft Academic Search

The anaerobic degradation of n-alkenes by a sulphate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T was investigated. Results suggest that enzymes required for alkene degradation are inducible. Moreover, total cellular fatty acids of strain CV2803T were predominantly C-odd when the strain was grown on C-odd substrates and C-even when grown on C-even substrates. In addition to classical bacterial fatty acids, unusual 4-Me-17:1?11

Cristiana Cravo-Laureau; Agnès Hirschler-Réa; Robert Matheron; Vincent Grossi



Mercury Abundances and Isotopic Compositions in the Murchison (CM) and Allende (CV)Carbonaceous Chondrites  

NASA Technical Reports Server (NTRS)

The abundance and isotopic composition of Hg was determined in bulk samples of both the Murchison (CM) and Allende (CV) carbonaceous chondrites using single- and multi-collector inductively coupled plasma mass spectrometry (ICP-MS). The bulk abundances of Hg are 294 6 15 ng/g in Murchison and 30.0 6 1.5 ng/g in Allende. These values are within the range of previous measurements of bulk Hg abundances by neutron activation analysis (NAA). Prior studies suggested that both meteorites contain isotopically anomalous Hg, with d l 96/202Hg values for the anomalous, thermal-release components from bulk samples ranging from 2260 %o to 1440 9/00 in Murchison and from 2620 9/00 to 1540 9/00 in Allende (Jovanovic and Reed, 1976a; 1976b; Kumar and Goel, 1992). Our multi-collector ICP-MS measurements suggest that the relative abundances of all seven stable Hg isotopes in both meteorites are identical to terrestrial values within 0.2 to 0.5 9/00m. On-line thermal-release experiments were performed by coupling a programmable oven with the singlecollector ICP-MS. Powdered aliquots of each meteorite were linearly heated from room temperature to 900 C over twenty-five minutes under an Ar atmosphere to measure the isotopic composition of Hg released fiom the meteorites as a h c t i o n of temperature. In separate experiments, the release profiles of S and Se were determined simultaneously with Hg to constrain the Hg distribution within the meteorites and to evaluate the possibility of Se interferences in previous NAA studies. The Hg-release patterns differ between Allende and Murchison. The Hg-release profile for Allende contains two distinct peaks, at 225" and 343"C, whereas the profile for Murchison has only one peak, at 344 C. No isotopically anomalous Hg was detected in the thermal-release experiments at a precision level of 5 to 30 9/00, depending on the isotope ratio. In both meteorites the Hg peak at ;340"C correlates with a peak in the S-release profile. This correlation suggests that Hg is associated with S-bearing phases and, thus, that HgS is a major Hg-bearing phase in both meteorites. The Hg peak at 225 C for Allende is similar to release patterns of physically adsorbed Hg on silicate and metal grains.

Lauretta, D. S.; Klaue, B.; Blum, J. D.; Buseck, P. R.



A secondary origin of chondrule magnetization in the Allende CV carbonaceous chondrite  

NASA Astrophysics Data System (ADS)

Magnetic fields in the solar nebula may have played a key role in the radial transport of angular momentum and mass during the early accretional phase of the solar system. Chondrules and many calcium aluminum inclusions (CAIs), millimeter sized silicate objects found in most chondritic meteorites, were heated to high temperatures and cooled in the nebula and therefore may have recorded a thermoremanent magnetization (TRM) from the nebula field. Additionally, primitive magnetization in chondrules and CAIs may yield constraints about their mode of formation. However, any such primary magnetization may have been significantly altered during subsequent metamorphism and aqueous alteration on the parent asteroid. We performed two tests to determine the nebular origins of remanent magnetization in chondrules and refractory inclusions in the Allende CV3 carbonaceous chondrite: 1) a classic paleomagnetic conglomerate test to identify post-accretional remagnetization events and 2) a unidirectionality test of subsamples taken from individual chondrules and CAIs. We conducted individual measurements of mutually oriented chondrules, CAIs, and matrix as well as SQUID microscope maps of the magnetic fields of 30 ?m thin sections. All samples and thin sections were mutually oriented to within 5°. Our results confirm previous findings that all subsamples of the meteorite carry a unidirectional overprint blocked up to 260°-290°C (MT component). Chondrules and CAIs also carry a higher temperature (HT) remanence oriented in scattered directions unrelated to the direction of the MT overprint. We have confirmed that this HT magnetization is not an artifact of the demagnetization procedure but is a preterrestrial component. Measurements of subsamples of single chondrules and CAIs show that the HT magnetization is not unidirectional within each inclusion. Petrographic data suggests that most magnetic minerals in Allende were the product of parent body alteration. These facts suggest that the magnetization in Allende chondrules and CAIs is not a preaccretional TRM and is unlikely to record the nebular field. We suggest that preaccretional remanence in Allende material was destroyed by aqueous alteration and metasomatism, which was followed or coincident with metamorphism that introduced the MT overprint. The HT component we detect may represent a chemical remanent magnetization (CRM) acquired during aqueous alteration.

Carporzen, L.; Fu, R.; Andrade Lima, E.; Weiss, B. P.



Opaque Mineral Assemblages at Chondrule Boundaries in the Vigarano CV Chondrite: Evidence for Gas-Solid Reactions Following Chondrule Formation  

NASA Technical Reports Server (NTRS)

Recent studies of opaque minerals in primitive ordinary chondrites suggest that metal grains exposed at chondrule boundaries were corroded when volatile elements recondensed after the transient heating event responsible for chondrule formation. Metal grains at chondrule boundaries in the Bishunpur (LL3.1) chondrite are rimmed by troilite and fayalite. If these layers formed by gas solid reaction, then the composition of the corrosion products can provide information on the chondrule formation environment. Given the broad similarities among chondrules from different chondrite groups, similar scale layers should occur on chondrules in other primitive meteorite groups. Here I report on metal grains at chondrule boundaries in Vigarano (CV3).

Lauretta, Dante S.



High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L. f. cv. Golden Leaves  

Microsoft Academic Search

Background  The tropical plant Ficus microcarpa L. f. cv. Golden Leaves (GL) is a high-light sensitive tropical fig tree in which sun-leaves are yellow and shade-leaves\\u000a are green. We compared the response of photosynthetic activities to strong light between GL and its wild-type (WT, Ficus microcarpa L. f.).\\u000a \\u000a \\u000a \\u000a \\u000a Results  Field measurements of maximum photosystem II (PSII) efficiency (F\\u000a v\\/F\\u000a m) of intact

Shunichi Takahashi; Ayumu Tamashiro; Yasuko Sakihama; Yasusi Yamamoto; Yoshinobu Kawamitsu; Hideo Yamasaki



Some regulatory aspects of [ 14 C]methylamine influx into Pisum sativum L. cv. Feltham First seedlings  

Microsoft Academic Search

[14C]Methylamine influx intoPisum sativum L. cv. Feltham First seedlings showed Michaelis-Menten-type kinetics with apparentVmax=49.2 µmol·g-1 FW·h-1 and apparentKm=0.51 mM. The competitive interactions between ammonium and methylamine were most obvious when biphasic kinetics were assumed with saturation of the first phase at ~0.05 mM. The inhibitor constant for ammonium (Ki)=0.027 mM. When [14C]methylamine was used in trace amounts with ammonium added

C. E. Deane-Drummond



Interpretation of F106B and CV580 in-flight lightning data and form factor determination  

NASA Technical Reports Server (NTRS)

Two topics of in-flight aircraft/lightning interaction are addressed. The first is the analysis of measured data from the NASA F106B Thunderstorm Research Aircraft and the CV580 research program run by the FAA and Wright-Patterson Air Force Base. The CV580 data was investigated in a mostly qualitative sense, while the F106B data was subjected to both statistical and quantitative analysis using linear triggered lightning finite difference models. The second main topic is the analysis of field mill data and the calibration of the field mill systems. The calibration of the F106B field mill system was investigated using an improved finite difference model of the aircraft having a spatial resolution of one-quarter meter. The calibration was applied to measured field mill data acquired during the 1985 thunderstorm season. The experimental determination of form factors useful for field mill calibration was also investigated both experimentally and analytically. The experimental effort involved the use of conducting scale models and an electrolytic tank. An analytic technique was developed to aid in the understanding of the experimental results.

Rudolph, T.; Horembala, J.; Eriksen, F. J.; Weigel, H. S.; Elliott, J. R.; Parker, S. L.; Perala, R. A.



Processor core for real time background identification of HD video based on OpenCV Gaussian mixture model algorithm  

NASA Astrophysics Data System (ADS)

The identification of moving objects is a fundamental step in computer vision processing chains. The development of low cost and lightweight smart cameras steadily increases the request of efficient and high performance circuits able to process high definition video in real time. The paper proposes two processor cores aimed to perform the real time background identification on High Definition (HD, 1920 1080 pixel) video streams. The implemented algorithm is the OpenCV version of the Gaussian Mixture Model (GMM), an high performance probabilistic algorithm for the segmentation of the background that is however computationally intensive and impossible to implement on general purpose CPU with the constraint of real time processing. In the proposed paper, the equations of the OpenCV GMM algorithm are optimized in such a way that a lightweight and low power implementation of the algorithm is obtained. The reported performances are also the result of the use of state of the art truncated binary multipliers and ROM compression techniques for the implementation of the non-linear functions. The first circuit has commercial FPGA devices as a target and provides speed and logic resource occupation that overcome previously proposed implementations. The second circuit is oriented to an ASIC (UMC-90nm) standard cell implementation. Both implementations are able to process more than 60 frames per second in 1080p format, a frame rate compatible with HD television.

Genovese, Mariangela; Napoli, Ettore



The change of fruit quality parameters in day-neutral strawberries cv. Diamante grown out of season.  


This research was conducted on the strawberry cultivar Diamante in continental parts of Croatia. The experiment was designed as four replicates with 100 plants for each harvest time. The quality of strawberries cv. Diamante grown out of season (fruit were picked in the early morning hours at three harvest times: 27 June, 7 September, 28 October) was determined. Total soluble solids ( degrees Brix) ranged from 5.00% to 7.03%, total acidity ranged from 1.05% to 1.12%, total soluble solids/acidity ranged from 4.74 to 6.34, vitamin C ranged from 40.73 mg/100 g fresh weight to 46.68 mg/100 g fresh weight, pH ranged from 3.53 to 3.72, total phenols ranged from 276.48 to 326.76 mg/l, non-flavonoids ranged from 169.50 to 260.53 mg/l, and flavonoids ranged from 44.71 to 106.53 mg/l, respectively. In addition, total anthocyanins ranged from 15.95 to 21.70 mg/l. The lightness L values of fruits ranged from 30.24 to 41.05, chroma C values from 25.92 to 34.13, and Hue angle H values from 44.51 to 56.02. The data showed that cv. Diamante grown out of season is of satisfactory quality. PMID:18982509

Voca, Sandra; Dobricevic, Nadica; Druzic, Jasmina; Duralija, Boris; Babojelic, Martina Skendrovic; Dermisek, Darijo; Cmelik, Zlatko



Discovery of a New FUN CAI from a CV Carbonaceous Chondrite: Evidence for Multistage Thermal Processing in the Protoplanetary Disk  

NASA Astrophysics Data System (ADS)

We report the mineralogy, petrography, as well as oxygen and magnesium isotope data of a newly identified FUN inclusion from the CV carbonaceous chondrite NWA 779. Variability in the texture of the mineral phases coupled with oxygen isotope data provides evidence for multistage evolution of this inclusion under distinct thermal regimes: slow crystallization of 16O-rich melt accompanied by evaporation, and subsequent remelting in an 16O-poor reservoir during transient heating events, possibly associated with the formation of CV chondrules. The inferred oxygen isotope composition of the precursor material of this inclusion (?17,18O=-48.4+2.5-3.0‰) is consistent with that observed for CAIs and amoeboid olivine aggregates from least metamorphosed carbonaceous chondrites, suggesting that both FUN and normal CAIs formed in an 16O-rich reservoir with oxygen isotope composition similar to that inferred for the Sun. However, in contrast to normal CAIs, most FUN inclusions show no evidence for live 26Al at the time of their formation. Based on these observations, we propose that the protosolar molecular cloud was polluted with stellar-derived 26Al prior to its collapse. Thus, FUN CAIs formed at a time when dust inherited from the molecular cloud-including the carrier of 26Al-was still poorly homogenized in the protoplanetary disk.

Thrane, Kristine; Nagashima, Kazuhide; Krot, Alexander N.; Bizzarro, Martin



Anti-CV2 associated cerebellar degeneration after complete response to chemoradiation of head and neck carcinoma.  


Paraneoplastic cerebellar degeneration is a rare neurological disorder that frequently precedes the detection of malignancy. Here, we report the case of a 60 year-old woman with locally advanced squamous cell carcinoma of the tongue who developed a subacute cerebellar syndrome associated with the presence of anti-CV2/CRMP5 antibodies in the cerebrospinal fluid, after achieving complete remission of the primary tumor and the involved cervical lymph nodes by chemoradiation. The patient's symptoms on presentation were dizziness and gait unsteadiness. On examination she showed dysarthria, nystagmus and limb and gait ataxia. The diagnosis of paraneoplastic cerebellar syndrome was made on the basis of the clinical findings and immunological testing that revealed the presence of anti-CV2/CRMP5 antibodies in the patient's cerebrospinal fluid. This syndrome, which is very rare in association with head and neck cancer, commonly precedes the detection of malignancy by a year or more and has been documented in only a few cases after completion of anticancer treatment. PMID:19798470

Saloustros, Emmanouel; Zaganas, Ioannis; Mavridis, Michail; Vamvakas, Lambros; Plaitakis, Andreas; Georgoulias, Vassilis; Mavroudis, Dimitris



Description of Gas-Phase Ion/Neutral Interactions in Differential Ion Mobility Spectrometry: CV Prediction Using Calibration Runs  

NASA Astrophysics Data System (ADS)

Differential ion mobility spectrometry (DMS) coupled to mass spectrometry is increasingly used in both quantitative analyses of biological samples and as a means of removing background interferences for enhanced selectivity and improved quality of mass spectra. However, DMS separation efficiency using dry inert gases often lacks the required selectivity to achieve baseline separation. Polar gas-phase modifiers such as alcohols are therefore frequently employed to improve selectivity via clustering/declustering processes. The choice of an optimal modifier currently relies on trial and error experiments, making method development a tedious activity. It was the goal of this study to establish a means of CV prediction for compounds using a homologous series of alcohols as gas-phase modifiers. This prediction was based on linear regression of compensation voltages of two calibration runs for the alcohols with the lowest and the highest molecular weights and readily available descriptors such as proton affinity and gas phase acidity of the modifier molecules. All experiments were performed on a commercial quadrupole linear ion trap mass spectrometer equipped with a DMS device between electrospray ionization source and entrance quadrupole lens. We evaluated our approach using a homologous series of 4-alkylbenzoic acids and a selection of 23 small molecules of high chemical diversity. Predicted CV values typically deviated from the experimentally determined values by less than 0.5 V. Several test compounds changed their ion mobility behavior for the investigated gas phase modifiers (e.g., from type B to type A) and thus could thus not be evaluated.

Auerbach, David; Aspenleiter, Julia; Volmer, Dietrich A.



Growth and cellular fatty-acid composition of a sulphate-reducing bacterium, Desulfatibacillum aliphaticivorans strain CV2803T, grown on n-alkenes.  


The anaerobic degradation of n-alkenes by a sulphate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T was investigated. Results suggest that enzymes required for alkene degradation are inducible. Moreover, total cellular fatty acids of strain CV2803T were predominantly C-odd when the strain was grown on C-odd substrates and C-even when grown on C-even substrates. In addition to classical bacterial fatty acids, unusual 4-Me-17:1delta11 and 4-Me-18:1delta11 fatty acids and their saturated homologues were detected when strain CV2803T was grown on 1-pentadecene and 1-hexadecene, respectively. These methyl-branched monounsaturated fatty acids could constitute specific metabolites of n-alkene degradation by sulphate-reducing bacteria. PMID:15344818

Cravo-Laureau, Cristiana; Hirschler-Réa, Agnès; Matheron, Robert; Grossi, Vincent



Al-26, Pu-244, Ti-50, REE, and trace element abundances in hibonite grains from CM and CV meteorites  

NASA Technical Reports Server (NTRS)

Hibonites from the CM meteorites Murchison, Murray, and Cold Bokkeveld, and hibonites and Ti-rich pyroxene from the CV chondrite Allende are studied. Electron microprobe measurements of major element concentrations and track and ion probe measurements of Mg and Ti isotopic ratios, rare earth elements (REEs), and trace element abundances are analyzed. Correlations between isotopic anomalies in Ti, Al-26, Pu-244, and Mg-26(asterisk) are examined. Ti isotopic anomalies are compared with REE and trace element abundance patterns. Reasons for the lack of Al-26 in the hibonites are investigated and discussed. It is observed that there is no correlation between the Ti isotopic compositions, and the presence of Mg-26(asterisk), Pu-244, and REE and trace element patterns in individual hibonite samples. The data reveal that hibonites are not interstellar dust grains but formed on a short time scale and in localized regions of the early solar system.

Fahey, A. J.; Mckeegan, K. D.; Zinner, E.; Goswami, J. N.



Al-26, Pu-244, Ti-50, REE, and trace element abundances in hibonite grains from CM and CV meteorites  

NASA Astrophysics Data System (ADS)

Hibonites from the CM meteorites Murchison, Murray, and Cold Bokkeveld, and hibonites and Ti-rich pyroxene from the CV chondrite Allende are studied. Electron microprobe measurements of major element concentrations and track and ion probe measurements of Mg and Ti isotopic ratios, rare earth elements (REEs), and trace element abundances are analyzed. Correlations between isotopic anomalies in Ti, Al-26, Pu-244, and Mg-26(asterisk) are examined. Ti isotopic anomalies are compared with REE and trace element abundance patterns. Reasons for the lack of Al-26 in the hibonites are investigated and discussed. It is observed that there is no correlation between the Ti isotopic compositions, and the presence of Mg-26(asterisk), Pu-244, and REE and trace element patterns in individual hibonite samples. The data reveal that hibonites are not interstellar dust grains but formed on a short time scale and in localized regions of the early solar system.

Fahey, A. J.; Goswami, J. N.; McKeegan, K. D.; Zinner, E.



Ion temperature and radial profile of CII-CV located in the edge and divertor plasmas of large helical device  

NASA Astrophysics Data System (ADS)

Space-resolved vacuum ultraviolet (VUV) spectroscopy using a 3-m normal incidence spectrometer is utilized to measure the impurity emission profile in the edge and divertor plasmas of the Large Helical Device (LHD). It measures the vertical profile of VUV lines emitted in the wavelength range of 300-3200 °A. CII, CIII, CIV, and CV lines emitted from carbon ions are successfully measured, and their ion temperatures are derived from the Doppler broadening. Vertical profiles of the emission intensity and the ion temperature are measured simultaneously for the CIV line. The emission intensity profile, which has several peak structures, is reasonably explained by considering the relation between the C3+ ion distribution and the geometry used for the observations.

Oishi, Tetsutarou; Morita, Shigeru; Dong, Chunfeng; Goto, Motoshi; Wang, Erhui; Huang, Xianli



Relationships between harvest time and wine composition in Vitis vinifera L. cv. Cabernet Sauvignon 1. Grape and wine chemistry.  


The study aimed to quantify the effects of grape maturity on wine alcohol, phenolics, flavour compounds and polysaccharides in Vitis vinifera L. cv Cabernet Sauvignon. Grapes were harvested at juice soluble solids from 20 to 26 °Brix which corresponded to a range of wine ethanol concentrations between 12% and 15.5%. Grape anthocyanin and skin tannin concentration increased as ripening progressed, while seed tannin declined. In the corresponding wines, monomeric anthocyanin and wine tannin concentration increased with harvest date, consistent with an enhanced extraction of skin-derived phenolics. In wines, there was an observed increase in yeast-derived metabolites, including volatile esters, dimethyl sulfide, glycerol and mannoproteins with harvest date. Wine volatiles which were significantly influenced by harvest date were isobutyl methoxypyrazine, C(6) alcohols and hexyl acetate, all of which decreased as ripening progressed. The implications of harvest date for wine composition is discussed in terms of both grape composition and yeast metabolism. PMID:23411300

Bindon, Keren; Varela, Cristian; Kennedy, James; Holt, Helen; Herderich, Markus



Monitoring melatonin and its isomer in Vitis vinifera cv. Malbec by UHPLC-MS/MS from grape to bottle.  


Several studies have shown the presence of melatonin and related compounds in grapes and wines. The latter provides evidence of the possibility to enhance the nutraceutical properties of premium wines. However, there are many external factors that can influence the levels of this indolamine in grape and wines. In this study, the monitoring of melatonin and its tentatively identified isomer was carried out during the entire winemaking process in Vitis vinifera cv. Malbec by ultra high-performance liquid chromatography-tandem mass spectrometry. Laboratory and pilot studies were carried out to elucidate the role of grape, yeasts, and tryptophan in the evolution of the indolamines during the fermentation process. Melatonin was detected in grape extract within the range 120-160 ng/g while its isomer was found in musts and finished wines. Our results demonstrate that Saccaromyces cervisiae plays a decisive role in contributing to the content of melatonin and its isomer in wine. PMID:22225625

Gomez, Federico José Vicente; Raba, Julio; Cerutti, Soledad; Silva, María Fernanda



In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.  


An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) ?-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana. PMID:24222501

Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert



Radiation-associated degenerative cardiovascular risks during normal aging and after adverse CV event 10 months post-initial exposure  

PubMed Central

Background: During the future exploration-type space missions, astronauts will be exposed to ionizing radiation (IR) for more than 1–2 years. The effect of cosmic IR during and after space flights on the cardiovascular (CV) system is unknown. Therefore, it is important to evaluate space IR effects on the CV system and determine potential post-mission degenerative excess relative risks (ERR) to the heart as a function of normal aging (IR + AGING model) as well as determine whether space IR may affect the processes of recovery after an adverse CV event (i.e. acute myocardial infarct, AMI) during normal aging (IR + AGING + AMI model). Methods: Nine-month-old C57BL6N male mice were IR once with proton (50 cGy, 1 GeV/n) or (56Fe 15 cGy, 1 GeV/n). IR-induced alterations in cardiac function were assessed by echocardiography (ECHO) and hemodynamic measurements (HEMO). AMI was induced by ligation of left anterior descending (LAD) coronary artery 10 months post-IR. Mice were monitored over 28 days post-AMI. Results: Compared with control, in the IR + AGING study group, left ventricular end-systolic pressure (LVESP) was significantly decreased in both 1H- and 56Fe-IR (P < 0.03, both), suggesting IR-associated decrease in contractile function 10 month post-IRs. However, compared with age-matched control mice (18 months), the LV end-diastolic pressure (LVEDP) was significantly increased (P < 0.05) and minimum LV pressure change (dP/dt min, mmHg/sec) was significantly decreased (P < 0.02) in 1H-IR but not 56Fe-IR mice, suggesting that a single 50 cGy full body 1H-IR decreases considerably the relaxation function of the heart 10 months post-AMI. Of note, an increase in LVEDP and a decrease in dP/dt min are indicators that heart is not pumping blood well and is an early independent prognostic CV risk factor for development of cardiac de-compensation. In all three IR + AGING + AMI study groups, in average, there was 10–15% mortality up to 3 days post-AMI surgery with ?90% survival rate in all groups 28 days post-AMI. This is rather very good survival rate for 18- to 20-month-old mice after permanent LAD ligation. In the IR + AGING + AMI study group, the most harmful effects on myocardial recovery 10 months post-IR and 28 days post-AMI were observed in the 56Fe-IR group. LVESP was significantly decreased in 56Fe-IR vs control and 1H-IR mice (P < 0.04 and <0.02, respectively). LVEDP was 3-fold higher in 56Fe-IR vs 1H-IR mice (P < 0.004) but was only slightly higher (P = n.s.) compared with control mice. However, dP/dt max and dP/dt min were significantly decreased in 56Fe-IR vs control (P < 0.007 and <0.05, respectively) and 1H-IR mice (P < 0.0004 and <0.0015, respectively), suggesting that 56Fe-AMI hearts developed cardiac de-compensation. Summary: Our data in the IR + AGING study group strongly suggest that 10 months post-IR low-dose high-energy 1H-IR but not low-dose HZE (56Fe) particle IR affects considerably contractile and relaxation functions during normal aging. Conversely, our data in the IR + AGING + AMI study group at 10 months post-IR taken together with our previously reported data for AMI recovery 3 month after a single 50 cGy 1H-IR and 15 cGy 56Fe-IR indicate that 3 months and as long as 10 months after a single full-body IR, the 56Fe-IR is detrimental, whereas 1H-IR does not have negative effects on post-AMI recovery. In fact, single 1H-IR, at this dose, was considerably beneficial for post-AMI recovery at 3 months, as well as at 10 months post-IR. Major conclusions: Our longitudinal 1, 3 and 10 months studies in the IR + AGING and IR + AGING + AMI groups reveal that a single full-body low-dose 1H and HZE particle radiation (56Fe) have long-lasting negative effect on heart homeostasis during normal aging (predominantly 56Fe and at 10 months 1H-IR, as well), and present a significant CV risk for recovery after adverse CV event (exclusively 56Fe-IR, whereas 1H-IR at this dose could beneficial). Further, the divergent effects of low dose 1H-IR vs 56Fe-IR on heart function duri

Sasi, Sharath P.; Yan, Xinhua; Lee, Juyong; Sisakyan, Hamayak; Carrozza, Joseph; Goukassian, David A.



CV -Teodoro Stadler, 2013 Teodoro Stadler -CV  

E-print Network

) Neuquen province, Argentina. 1995-2000 Project Manager "Integrated Pest Management of the Cotton Boll by the Common Fund for Commodities (Amsterdam) and supervised by the International Cotton Advisory Committee Anthonomus grandis (Coleoptera : Curculionidae) control and eradication. Design a insecticide resistance

Lawrence, Rick L.


L'aptitude au croisement avec le seigle (Secale cereale L.) d'une ligne hexaplode de Triticum (cv. Roazon rsul-  

E-print Network

-clés additionnels : Blé, Triticum aestivum, Trsticale, Gènes d'incompatibilité, lntrogression. SUMMARY Crossability. Additional key-words : Wheat, Triticum aestivum, Triticale, Cross-compatibility, Crossability genesL'aptitude au croisement avec le seigle (Secale cereale L.) d'une lignée hexaploïde de Triticum (cv

Paris-Sud XI, Université de


Environmental effect on ABA concentration and water potential in olive leaves ( Olea europaea L. cv “Koroneiki”) under non-irrigated field conditions  

Microsoft Academic Search

How does the olive tree respond to environmental stress in the Mediterranean climate under non-irrigated field conditions with respect to leaf abscisic acid (ABA) concentrations and water status? To answer this question we determined simultaneously ABA concentration and water potential (?l) in olive leaves (Olea europaea L. cv “Koroneiki”) during three successive years and related them to environmental parameters. The

C. K. Kitsaki; J. B. Drossopoulos



Nickolas Themelis: Director, Earth Engineering Center[8/19/2009 4:40:26 PM  

E-print Network

Nickolas Themelis: Director, Earth Engineering Center[8/19/2009 4:40:26 PM] The Earth Engineering Center of Columbia University KARL SZEKIELDA (212) 923. Szekielda was PI in the Earth Resources Technology Satellite under Skylab (EREP). Appointed National


Omega gliadin genes expressed in Triticum aestivum cv. Butte 86: Effects of post-anthesis fertilizer on transcript accumulation during grain development  

Microsoft Academic Search

The partial coding sequences of omega gliadin genes expressed in developing wheat kernels Triticum aestivum cv. Butte 86 were identified in databases of expressed sequence tags (ESTs). Three gene assemblies encode proteins with PQQPFP as the predominant repetitive motif. Of these, two encode proteins with at least one cysteine and thus may be incorporated into the glutenin polymer. Another two

Susan B. Altenbach; Kerry M. Kothari



A comparison of nine species of arbuscular mycorrhizal fungi on the development and nutrition of micropropagated Rubus idaeus L. cv. Glen Prosen (Red Raspberry)  

Microsoft Academic Search

Growth, development and nutrient status of micropropagated Rubus idaeus cv. Glen Prosen in response to inoculation with nine species of arbuscular mycorrhizal (AM) fungi from three different genera\\u000a was investigated. The nine species of AM fungi included, Glomus clarum, G. etunicatum, G. intraradices, Gigaspora rosea, Gi. gigantea, Gi. margarita, Scutellospora calospora, S. heterogama and S. persica. Plant responses to AM

Jeanette Taylor; Lucy Harrier



Free radical scavenging activity and phenolic content in achenes and thalamus from Fragaria chiloensis ssp. chiloensis, F. vesca and F. x ananassa cv. Chandler  

Microsoft Academic Search

The total phenolic, flavonoid and anthocyanin content of achenes (true fruit) and thalamus (receptacle) from the native South American Fragaria chiloensis ssp. chiloensis (f. patagonica and f. chiloensis), Fragaria vesca and Fragaria x ananassa cv. Chandler was determined by spectrophotometric means. Highest phenolic content was found in F. vesca while lowest content was measured for white strawberry (F. chiloensis ssp.

José Cheel; Cristina Theoduloz; Jaime A. Rodríguez; Peter D. S. Caligari; Guillermo Schmeda-Hirschmann



Influence of Plant Water Status on the Production of C 13 -Norisoprenoid Precursors in Vitis vinifera L. Cv. Cabernet Sauvignon Grape Berries  

Microsoft Academic Search

The influence of irrigation strategy on grape berry carotenoids and C13-norisoprenoid precursors was investigated for Vitis vinifera L. cv. Cabernet Sauvignon. Two irrigation treatments were compared, one in which vines received reduced irrigation applied alternately to either side of the vine (partial rootzone drying, PRD) and a second control treatment in which water was applied to both sides of the

Keren A. Bindon; Peter R. Dry; Brian R. Loveys



Leaf pruning intensities at flowering of banana ( Musa AAA, cv. Grande Naine) did not influence fruit green and yellow life and quality  

Microsoft Academic Search

This study examined the effects of leaf pruning intensities at flowering on the green and yellow life and fruit quality of bananas (Musa AAA, cv. Grande Naine). The fruit from banana plants that retained 7, 9, 11 and 13 leaves after pruning were packed in carton boxes of 13.7kg and stored in a cold room at 14°C for 21 days

Maricruz Ramírez; Marco Vinicio Sáenz; Alfonso Vargas; Mario Araya



Draft Genome Sequence of Flavobacterium sp. Strain F52, Isolated from the Rhizosphere of Bell Pepper (Capsicum annuum L. cv. Maccabi)  

PubMed Central

Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

Kolton, Max; Green, Stefan J.; Harel, Yael Meller; Sela, Noa; Elad, Yigal



Callus initiation and plant regeneration from inflorescence primordia of the intergeneric hybrid Agropyron repens (L.) Beauv.x Bromus inermis Leyss. cv. nanus on a modified nutritive medium  

Microsoft Academic Search

Plant regeneration from callus of intergeneric hybrid Agropyron repens (L.) Beauv. x Bromus inermis Leyss cv. nanus (AGROMUS) was carried out on a new culture medium designated medium-F. Within 21 days of the plating of inflorescence primordia the initiated callus showed globular structures. From the 21st day of culture, one step plant regeneration occurred on the callus without subculture. The

G. Gyulai; J. Janovszky; E. Kiss; L. Lelik; A. Csillag; L. E. Heszky




Microsoft Academic Search

A b s t r a c t . The results of leaf chlorophyll fluoresc ence measurements of crop oat cv. Akt and wild oat subjected to Dicuran 80 WP (a.s. chlortoluron) herbicide with two adjuvants (Adpros 85 SL, Atpolan 80 EC) are presented. Chlortoluron, as a phenyl-ure a herbicide, is an inhibitor of photosynthetic elec tron transport in photosystem

Antoni Murkowski


Case 1:13-cv-00372-KD-M Document 10 Filed 10/02/13 Page 1 of 33 IN THE UNITED STATES DISTRICT COURT  

E-print Network

Case 1:13-cv-00372-KD-M Document 10 Filed 10/02/13 Page 1 of 33 IN THE UNITED STATES DISTRICT COURT FOR THE SOUTHERN DISTRICT OF ALABAMA SOUTHERN DIVISION UNITED STATES OF AMERICA; ALABAMA DEPARTMENT, the United States of America ("United States"), on behalf of the Secretary of the United States Department


Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): tools for structural and functional genomics  

Microsoft Academic Search

BACKGROUND: The creation of minimally redundant tile paths (hereafter MTP) from contiguous sets of overlapping clones (hereafter contigs) in physical maps is a critical step for structural and functional genomics. Build 4 of the physical map of soybean (Glycine max L. Merr. cv. 'Forrest') showed the 1 Gbp haploid genome was composed of 0.7 Gbp diploid, 0.1 Gbp tetraploid and

JL Shultz; C Yesudas; S Yaegashi; AJ Afzal; S Kazi; DA Lightfoot




EPA Science Inventory

Animal models of coronary heart disease (e.g., hyperlipidemic rabbits) are being used to investigate epidemiologic associations between higher levels of air pollution and adverse CV consequences. Mechanisms by which pollutant-induced lung or systemic inflammation leads to acute C...


Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability  

PubMed Central

Background Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation. Reviewer This article was reviewed by Sándor Pongor, L. Aravind and Anthony M. Poole. PMID:23324115



Long-term fungal inhibitory activity of water-soluble extracts of Phaseolus vulgaris cv. Pinto and sourdough lactic acid bacteria during bread storage.  


The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts of L. brevis AM7 sourdough and P. vulgaris cv. Pinto had a MIC of 40 mg of peptide/ml and 30.9 mg of protein/ml, respectively. MICs were markedly lower when chemically synthesized peptides or partially purified protein fractions were used. The water-soluble extract of P. vulgaris cv. Pinto showed inhibition toward a large number of fungal species isolated from bakeries. Phaseolin alpha-type precursor, phaseolin, and erythroagglutinating phytohemagglutinin precursor were identified in the water-soluble extract of P. vulgaris cv. Pinto by nano liquid chromatography-electrospray ionization-tandem mass spectrometry. When the antifungal activity was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all three proteins were inhibitory. A mixture of eight peptides was identified from the water-soluble extract of sourdough L. brevis AM7, and five of these exhibited inhibitory activity. Bread was made at the pilot plant scale by sourdough fermentation with L. brevis AM7 and addition of the water-soluble extract (27%, vol/wt; 5 mg of protein/ml) of P. vulgaris cv. Pinto. Slices of bread packed in polyethylene bags did not show contamination by fungi until at least 21 days of storage at room temperature, a level of protection comparable to that afforded by 0.3% (wt/wt) calcium propionate. PMID:18849463

Coda, Rossana; Rizzello, Carlo G; Nigro, Franco; De Angelis, Maria; Arnault, Philip; Gobbetti, Marco



CV-Dust: Atmospheric aerosol in the Cape Verde region: carbon and soluble fractions of PM10  

NASA Astrophysics Data System (ADS)

Every year, billions of tons of eroded mineral soils from the Saharan Desert and the Sahel region, the largest dust source in the world, cross Mediterranean towards Europe, western Asia and the tropical North Atlantic Ocean as far as the Caribbean and South America. Many aspects of the direct and indirect effects of dust on climate are not well understood and the bulk and surface chemistry of the mineral dust particles determines interactions with gaseous and other particle species. The quantification of the magnitude of warming or cooling remains open because of the strong variability of the atmospheric dust burden and the lack of representative data for the spatial and temporal distribution of the dust composition. CV-Dust is a project that aims at provide a detailed data on the size distribution and the size-resolved chemical and mineralogical composition of dust emitted from North Africa using a natural laboratory like Cape Verde. This archipelago is located in an area of massive dust transport from land to ocean, and is thus ideal to set up sampling devices that are able to characterize and quantify dust transported from Africa. Moreover, Cape Verde's future economic prospects depend heavily on the encouragement of tourism, therefore it is essential to elucidate the role of Saharan dust may play in the degradation of Cape Verde air quality. The main objectives of CV-Dust project are: 1) to characterize the chemical and mineralogical composition of dust transported from Africa by setting up an orchestra of aerosol sampling devices in the strategic archipelago of Cape Verde; 2) to identify the sources of particles in Cape Verde by using receptor models; 3) to elucidate the role Saharan dust may play in the degradation of Cape Verde air quality; 4) to model processes governing dust production, transport, interaction with the radiation field and removal from the atmosphere. Here we present part of the data obtained throughout the last year, involving a set of more than 100 PM10 samples, addressing mainly their mass concentrations and the chemical composition of water soluble ions and carbon species (carbonates and organic and elemental carbon). Different PM10 samplers worked simultaneously in order to collect enough mass to make the aerosol characterization through the different methodologies and to collect aerosols in different filter matrixes, which have to be appropriated to the chemical and mineralogical analysis. The sampling site was located at Santiago Island, in the surroundings of Praia City (14° 55' N e 23° 29' W, 98 m at sea level). High concentrations, up to more than 400 ?g m-3, are connected to north-east and north-northeast winds, and it was identified several dust events characteristic of "bruma seca", whose duration is on average of two to four days. Backward trajectories analysis confirms that the high concentrations in Cape Verde are associated with air masses passing over the Sahara. During dust events the percentage of inorganic water soluble ions for the total PM10 mass concentration decreased significantly to values lower than 10% in comparison with remainder data that range around 45±10%. Acknowledgement: This work was funded by the Portuguese Science Foundation (FCT) through the project PTDD/AAC-CLI/100331/2008 and FCOMP-01-0124-FEDER-008646 (CV-Dust). J. Cardoso acknowledges the PhD grant SFRH-BD-6105-2009 from FCT.

Pio, C.; Nunes, T.; Cardoso, J.; Caseiro, A.; Custódio, D.; Cerqueira, M.; Patoilo, D.; Almeida, S. M.; Freitas, M. C.



Ultrastructural changes and the distribution of arabinogalactan proteins during somatic embryogenesis of banana (Musa spp. AAA cv. 'Yueyoukang 1').  


A better understanding of somatic embryogenesis in banana (Musa spp.) may provide a practical way to improve regeneration of banana plants. In this study, we applied scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to visualize the ultrastructural changes during somatic embryogenesis of banana (Musa AAA cv. 'Yueyoukang 1'). We also used histological and immunohistochemical techniques with 16 monoclonal antibodies to study the spatial distribution and cellular/subcellular localization of different arabinogalactan protein (AGP) components of the cell wall during somatic embryogenesis. Histological study with periodic acid-Schiff staining documented diverse embryogenic stages from embryogenic cells (ECs) to the late embryos. SEM revealed a mesh-like structure on the surface of proembryos which represented an early structural marker of somatic embryogenesis. TEM showed that ECs were rich in juvenile mitochondria, endoplasmic reticulum and Golgi stacks. Cells in proembryos and early globular embryos resembled ECs, but they were more vacuolated, showed more regular nuclei and slightly more developed organelles. Immunocytochemical study revealed that the signal of most AGP epitopes was stronger in starch-rich cells when compared with typical ECs. The main AGP component in the extracellular matrix surface network of banana proembryos was the MAC204 epitope. Later, AGP immunolabelling patterns varied with the developmental stages of the embryos. These results about developmental regulation of AGP epitopes along with developmental changes in the ultrastructure of cells are providing new insights into the somatic embryogenesis of banana. PMID:21496030

Pan, Xiao; Yang, Xiao; Lin, Guimei; Zou, Ru; Chen, Houbin; Samaj, Jozef; Xu, Chunxiang



Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3-year comparison.  


In continuation of our studies of altitudinal effects on secondary metabolite profile of flowering heads from taxa of the Asteraceae, we investigated phenolic contents and radical scavenging potential from cultivated plants of Arnica montana cv. ARBO during the growing seasons 2003, 2004, and 2005. By conducting experiments on potted plants, we excluded that differences in phenolic contents from plants grown at different altitudes were related primarily to differences in soil composition at these sites. To assess altitudinal and interseasonal variation, plants of A. montana cultivar ARBO were grown in nine experimental plots at altitudes between 590 and 2,230 m at Mount Patscherkofel near Innsbruck, Austria. In all growing seasons and regardless of the soil the plants were grown in, the proportion of flavonoids with vicinal-free hydroxy groups in ring B to flavonoids lacking this feature, and the total amount of caffeic acid derivatives, significantly increased with elevation. These increases of antioxidant phenolics corresponded to an increase of the radical scavenging potential of extracts from plants grown at different altitudes. The results are discussed in regard to previous studies that suggest that enhanced UV-B radiation and decreased temperatures trigger augmented biosynthesis of UV-absorbing and antioxidant phenolics in higher plants. PMID:18259818

Spitaler, Renate; Winkler, Andrea; Lins, Isabella; Yanar, Sema; Stuppner, Hermann; Zidorn, Christian



Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening  

PubMed Central

Background Cluster thinning is an agronomic practice in which a proportion of berry clusters are removed from the vine to increase the source/sink ratio and improve the quality of the remaining berries. Until now no transcriptomic data have been reported describing the mechanisms that underlie the agronomic and biochemical effects of thinning. Results We profiled the transcriptome of Vitis vinifera cv. Sangiovese berries before and after thinning at veraison using a genome-wide microarray representing all grapevine genes listed in the latest V1 gene prediction. Thinning increased the source/sink ratio from 0.6 to 1.2 m2 leaf area per kg of berries and boosted the sugar and anthocyanin content at harvest. Extensive transcriptome remodeling was observed in thinned vines 2 weeks after thinning and at ripening. This included the enhanced modulation of genes that are normally regulated during berry development and the induction of a large set of genes that are not usually expressed. Conclusion Cluster thinning has a profound effect on several important cellular processes and metabolic pathways including carbohydrate metabolism and the synthesis and transport of secondary products. The integrated agronomic, biochemical and transcriptomic data revealed that the positive impact of cluster thinning on final berry composition reflects a much more complex outcome than simply enhancing the normal ripening process. PMID:22192855



Response of different maturity stages of sapota (Manilkara achras Mill.) cv. Kallipatti to in-package ethylene absorbent.  


Sapota fruits are highly perishable due to their climacteric nature. The rapid softening of fruits is primarily due to high activity of many oxidative enzymes and liberation of ethylene. Harvest maturity plays a crucial role in deciding the marketability of climacteric fruits in general. Attempt has been made to evaluate the response of ethylene absorbent on variable maturity groups of harvested Sapota cv. Kallipatti with the objective to delay the ripening during transit and extend its marketability during storage at ambient condition (27-32 °C & 65-75% R.H.). Harvested fruits having three different degree of ripeness (as maturity indices viz. mature, half-ripe and ripe) were packed with or without ethylene absorbent sachets (Bioconservación, France) in 10 kg CFB boxes and transported from Dahnu to Delhi covering a distant of approximately 2500 KM by truck on road along with conventional packaging as control. The fruits were evaluated immediately on arrival at Delhi and subsequently during storage for various physical, physiological, biochemical and decay parameters. Mature fruits with ethylene absorbent exhibited maximum delay in ripening, low ethylene liberation, weight loss and high fruit firmness. The response of ethylene absorbent to extend the marketability of ripe fruit was not significant. PMID:23572819

Bhutia, Wangdup; Pal, R K; Sen, Sangita; Jha, S K



Intake and Performance of Yearling Steers Grazing Guineagrass (Panicum maximum cv. Tanzânia) Pasture Supplemented with Different Energy Sources  

PubMed Central

The aim of this study was to evaluate the efficiency of supplements containing different energy sources in relation to mineral supplementation of steers grazing guineagrass (Panicum maximum cv Tanzânia) pasture, during the dry season. The experimental design was a randomized block with three treatments and four replications. The treatments consisted of a mineral supplementation and two other supplements, one based on corn seed and the other based on soybean hulls, and provided at 0.8% of body weight. Forty-eight, 12 month-old crossbred steers with an average initial body weight of 267 kg, were assigned to twelve paddocks (1,125 ha) of guineagrass. The animals that were fed with soybean hulls and corn seed presented a greater average daily gain (0.982 and 0.937) when compared with the mineral supplementation. Soybean hulls can be used as a satisfactory food source, replacing corn as an energy source in the supplementation of beef cattle without compromising animal performance. PMID:25049797

Santana, M. C. A.; Euclides, V. B. P.; Mancio, A. B.; Medeiros, S. R.; Costa, J. A. R.; Oliveira, R. L.



Analysis of crude protein and allergen abundance in peanuts (Arachis hypogaea cv. Walter) from three growing regions in Australia.  


The effects of plant growth conditions on concentrations of proteins, including allergens, in peanut ( Arachis hypogaea L.) kernels are largely unknown. Peanuts (cv. Walter) were grown at five sites (Taabinga, Redvale, Childers, Bundaberg, and Kairi) covering three commercial growing regions in Queensland, Australia. Differences in temperature, rainfall, and solar radiation during the growing season were evaluated. Kernel yield varied from 2.3 t/ha (Kairi) to 3.9 t/ha (Childers), probably due to differences in solar radiation. Crude protein appeared to vary only between Kairi and Childers, whereas Ara h 1 and 2 concentrations were similar in all locations. 2D-DIGE revealed significant differences in spot volumes for only two minor protein spots from peanuts grown in the five locations. Western blotting using peanut-allergic serum revealed no qualitative differences in recognition of antigens. It was concluded that peanuts grown in different growing regions in Queensland, Australia, had similar protein compositions and therefore were unlikely to show differences in allergenicity. PMID:23495786

Walczyk, Nicole E; Smith, Penelope M C; Tovey, Euan; Wright, Graeme C; Fleischfresser, Dayle B; Roberts, Thomas H



Effect of organic matter additions on uptake of weathered DDT by Cucurbita pepo ssp. pepo cv. Howden.  


Greenhouse studies were conducted to assess the impact of organic matter additions on plant uptake of DDT [2,2-bis(chlorophenyl)-1,1,1-trichloroethane] from weathered soil. Cucurbita pepo ssp. pepo cv. Howden pumpkins were grown in 100 g of DDT contaminated soil ([DDT] - 1100 ng/g) mixed with equal volumes of either clean soil, perlite, vermiculite, peat, potting soil, or granular activated carbon (GAC) to give total organic carbon contents of 2.4%, 2.5%, 2.6%, 11.5%, 12.2%, and 27.3%, respectively. As in other studies, root DDT concentrations were significantly lower in soils with high organic matter. Root bioaccumulation factors (BAF = [DDT]root/[DDT]soil) approximated this trend. Root concentrations correlated with organic matter concentrations and not with soil DDT concentrations. Conversely, shoot DDT concentrations, shoot BAFs and translocation factors (TLF = BAF(shoot)/BAF(root)) were not significantly different between treatment groups, except for plants grown in GAC/DDT soil. This suggests that amendments with a range of organic matter contents may be added to improve soil conditions at industrial sites without significant adverse effects on phytoextraction potential of C. pepo ssp. pepo. PMID:20734916

Lunney, Alissa I; Rutter, Allison; Zeeb, Barbara A



Overexpression, purification and enzymatic characterization of a recombinant plastidial glucose-6-phosphate dehydrogenase from barley (Hordeum vulgare cv. Nure) roots.  


In plant cells, the plastidial glucose 6-phosphate dehydrogenase (P2-G6PDH, EC represents one of the most important sources of NADPH. However, previous studies revealed that both native and recombinant purified P2-G6PDHs show a great instability and a rapid loss of catalytic activity. Therefore it has been difficult to describe accurately the catalytic and physico-chemical properties of these isoforms. The plastidial G6PDH encoding sequence from barley roots (Hordeum vulgare cv. Nure), devoid of a long plastidial transit peptide, was expressed as recombinant protein in Escherichia coli, either untagged or with an N-terminal his-tag. After purification from both the soluble fraction and inclusion bodies, we have explored its kinetic parameters, as well as its sensitivity to reduction. The obtained results are consistent with values determined for other P2-G6PDHs previously purified from barley roots and from other land plants. Overall, these data shed light on the catalytic mechanism of plant P2-G6PDH, summarized in a proposed model in which the sequential mechanism is very similar to the mammalian cytosolic G6PDH. This study provides a rational basis to consider the recombinant barley root P2-G6PDH as a good model for further kinetic and structural studies. PMID:24161756

Cardi, Manuela; Chibani, Kamel; Castiglia, Daniela; Cafasso, Donata; Pizzo, Elio; Rouhier, Nicolas; Jacquot, Jean-Pierre; Esposito, Sergio



Early inhibition of photosynthesis during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14  

SciTech Connect

Early physiological effects of developing Mn toxicity in young leaves of burley tobacco (Nicotiana tabacum L. cv KY 14) were examined in glass-house/water cultured plants grown at high (summer) and low (winter) photon flux. Following transfer of plants to solutions containing 1 millimolar Mn{sup 2+}, sequential samplings were made at various times for the following 9 days, during which Mn accumulation by leaves increased rapidly from {approx} 70 on day 0 to {approx} 1700 and {approx} 5000 microgram per gram dry matter after 1 and 9 days, respectively. In plants grown at high photon flux, net photosynthesis declined by {approx} 20 and {approx} 60% after 1 and 9 days, respectively, and the onset of this decline preceded appearance (after 3 to 4 days) of visible foliar symptoms of Mn toxicity. Intercellular CO{sub 2} concentrations and rates of transpiration were not significantly affected. Though the activity of latent or activated polyphenol oxidase increased in parallel with Mn accumulation, neither leaf respiration nor the activity of catalase (EC and peroxidase (EC were greatly affected. These effects from Mn toxicity could not be explained by any changes in protein or chlorophyll abundance. Additionally, they were not a consequence of Mn induced Fe deficiency. Therefore, inhibition of net photosynthesis and enhancement of polyphenol oxidase activity are early indicators of excess Mn accumulation in tobacco leaves.

Nable, R.O.; Houtz, R.L.; Cheniae, G.M. (Univ. of Kentucky, Lexington (USA))



Effect of Calcium Nitrate and Boric Acid Sprays on Fruit Set, Yield and Fruit Quality of cv. Amhat Date Palm  

E-print Network

Abstract: The objective of the present study is to investigate the impact of spraying boric acid and/or calcium nitrate on fruit set, yield and fruit of cv. Amhat date palm. The present study was carried out during 2011/2012 growing seasons. Palms were sprayed with boric acid at 250 and 500 ppm and calcium nitrate at 1 and 2 % as individual application or in a combination between boric acid and calcium nitrate concentrations treatments. In general, results indicated that spraying date palm inflorescence with both boric acid and/or calcium nitrate had a significant effect on fruit set, yield and fruit physical and chemical characteristics of Amhat date palm. The superior treatment concerning yield and fruit quality was spraying boric acid at 500 ppm combined with calcium nitrate at 2 % in the two experimental seasons. Key words: Date palm Fruit set Yield Fruit quality Boric acid Calcium nitrate INTRODUCTION synthesis, transport of sugars and carbohydrate metabolism [12]. Calcium is conceder as one of the most Date palm (Phoenix dactylifera L.) is concerned as important minerals determining the quality of fruit since it one of the important crops in arid and semi-arid regions of is required for cell elongation and cell division [13]. Till the world. Date palm is one of the ancient domestic fruit now, a little attention have been paid towards nutrient

S. M. A. Sarrwy; E. G. Gadalla; E. A. M. Mostafa


Identification of Proteins Correlated with Increased Freezing Tolerance in Bromegrass (Bromus inermis Leyss. cv Manchar) Cell Cultures.  


Cellular and extracellular protein profiles from Bromus inermis Leyss. cv Manchar cell suspension cultures cold hardened by low temperature and abscisic acid (ABA) treatment were analyzed by one- and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Cellular proteins (25, 165, 190, and 200 kilodaltons) increased by low temperature growth and cellular proteins (20, 25, 28, 30, 32, 37, 40, 45, 200 kilodaltons) increased by exogenous ABA treatment were identified. Low temperature treatment inhibited the synthesis of a 22 kilodalton protein and ABA treatment resulted in the synthesis of two extracellular proteins (17 and 21 kilodaltons). Low temperature and ABA-induced hardening conditions increased or induced a 25 and a 200 kilodalton protein. The 25 and a 30 kilodalton protein previously shown to be enriched by ABA-induced hardening conditions at both 3 and 23 degrees C temperatures co-fractionated with the crude membrane fraction (30,000g sediment). The 200 kilodalton protein was detected in the 30,000g supernatant. Two-dimensional analysis of the crude membrane fraction resolved the 30 kilodalton protein band into a major polypeptide with an apparent isoelectric point of 6.85. PMID:16665909

Robertson, A J; Gusta, L V; Reaney, M J; Ishikawa, M



Effect of silver nanoparticles on rice (Oryza sativa L. cv. KDML 105) seed germination and seedling growth.  


With the advances in nanotechnology, silver nanoparticles (AgNPs) have been applied in many industries, increasing their potential exposure level in the environment, yet their environmental safety remains poorly evaluated. The possible effects of different sized AgNPs (20, 30-60, 70-120 and 150nm diameter) on jasmine rice, Oryza sativa L. cv. KDML 105, were investigated at different concentrations (0.1, 1, 10, 100 and 1000mg/L) upon seed germination and seedling growth. The results revealed that the level of seed germination and subsequent growth of those seedlings that germinated were both decreased with increasing sizes and concentrations of AgNPs. Based on the analysis of AgNPs accumulation in plant tissues, it implied that the higher uptake was found when the seeds were treated with the smaller AgNPs, 20nm diameter AgNPs, but it was trapped in the roots rather than transported to the leaves. These resulted in the less negative effects on seedling growth, when compared to the seed soaking with the larger AgNPs with 150nm diameter. The negative effects of AgNPs were supported by leaf cell deformation when rice seeds were treated with 150-nm-diameter AgNP at the concentration of 10 or 100mg/L during seed germination. These results further strengthen our understanding of environmental safety information with respect to nanomaterials. PMID:24726943

Thuesombat, Pakvirun; Hannongbua, Supot; Akasit, Sanong; Chadchawan, Supachitra



Cadmium tolerance and accumulation characteristics of mature flax, cv. Hermes: contribution of the basal stem compared to the root.  


The potential of mature flax plants (cv. Hermes) to tolerate and accumulate cadmium (Cd) was studied to determine which part of the plant would be the key organ for phytoremediation purposes. After 4 month-growth on sand substrate containing 0.1mM Cd in a greenhouse, the roots and stems were separated and the stems were divided into three parts. The effects of Cd were studied on growth parameters, histology and mineral nutrition. No visible toxic symptoms were observed. Tolerance-index values calculated from growth parameters and nutrients remained relatively high, allowing the development of the plant until maturity and formation of seeds. The roots and bottom stem accumulated the highest quantity of Cd (750 and 360 mg/kg dry matter), values which largely exceeded the threshold defined for hyperaccumulators. On the other hand, basal stem had a high bioconcentration factor (BCF=32) and translocation factor TF' (2.5) but a low TF (0.5), indicating that this basal part would play a major role in phytoremediation (phytostabilization rather than phytorextraction). Therefore, the high tolerance to Cd and accumulation capacity make possible to grow Hermes flax on Cd-polluted soils. PMID:22858130

Douchiche, Olfa; Chaïbi, Wided; Morvan, Claudine



Dehydropyrrolizidine Alkaloids, Including Monoesters with an Unusual Esterifying Acid, from Cultivated Crotalaria juncea (Sunn Hemp cv.'Tropic Sun')  

PubMed Central

Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves and seeds of ‘Tropic Sun’ were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid content of the roots, stems, leaves of immature plants was estimated to be 0.05, 0.12 and 0.01% w/w respectively while seeds were estimated to contain 0.15% w/w. PMID:22429238

Colegate, Steven M.; Gardner, Dale R.; Joy, Robert J.; Betz, Joseph M.; Panter, Kip E.



Isolation and molecular characterization of an ethylene response factor NtERF1-1 in Nicotiana tabacum cv. Xanthi.  


Apetala2/Ethylene Response Factors (AP2/ERF) play important roles in regulating gene expression under abiotic and biotic stress in the plant kingdom. Here, we isolated a member of the AP2/ERF transcription factors, NtERF1-1, from Nicotiana tabcum cv. Xanthi NN carrying the N gene, which is resistant to Tobacco mosaic virus (TMV). NtERF1-1 encoded a putative protein of 229 amino acids with a predicted molecular mass of 24.58 kDa. Nucleotide sequence analysis showed that NtERF1-1 contained a conserved DNA-binding domain at the N-terminal. Comparison of amino acid sequences revealed that NtERF1-1 possessed high similarities to ERFs from diverse plants. Semi-quantitative and real-time quantitative RT-PCR analyses indicated that NtERF1-1 was up-regulated following TMV infection. In addition, we speculated that NtERF1-1 might participate in the signal transduction pathway of defence response inducted by the interaction between the N gene and TMV. PMID:25431417

Gao, Jun-Shan; Hu, Li; Xie, Peng; Meng, Yan; Cai, Yong-Ping; Lin, Yi



Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv.'Tropic Sun').  


Cultivation of Crotalaria juncea L. (Sunn Hemp cv. 'Tropic Sun') is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves, and seeds of 'Tropic Sun' were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems, and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid contents of the roots, stems, and leaves of immature plants were estimated to be 0.05, 0.12, and 0.01% w/w, respectively, whereas seeds were estimated to contain 0.15% w/w. PMID:22429238

Colegate, Steven M; Gardner, Dale R; Joy, Robert J; Betz, Joseph M; Panter, Kip E



Re-examination of the extraction of MOS interface-state density by C-V stretchout and conductance methods  

NASA Astrophysics Data System (ADS)

The extraction of interface-state density by the stretchout of MOS C-V (Terman method) is re-examined. It is shown that the typical 1 MHz frequency is not nearly high enough to get rid of the interface-state contribution to the MOS capacitance. When coupled with a bias-dependent trap time constant (?), this could result in a severe underestimate of the interface-state density. The ‘conductance method’, on the other hand, can extract the interface-state density accurately if the MOS is biased in depletion and if ? ˜ 1/? is within the measured frequency range. Also, the robustness of the conductance method subject to errors in the estimated oxide capacitance, as well as its extendibility into regions of weak and strong inversion is investigated. Furthermore, two cases of false peaks under the conductance method are mentioned: the first due to a small tunneling leakage in thin oxides and the second due to a high density of bulk-oxide traps.

Chen, Han-Ping; Yuan, Yu; Yu, Bo; Chang, Chih-Sheng; Wann, Clement; Taur, Yuan



Some observations of the effects of applied magnetic fields on growth of Avena coleoptiles. [Avena sativa cv. Garry  

SciTech Connect

Applied magnetic fields (5000-18,000 Gauss) disrupt the pattern of cellulose microfibril deposition in cells of Avena sativa cv. Garry coleoptiles. Freeze fracture reveals the microfibrils of the innermost wall layers to be randomly oriented, rather than parallel and transverse to the elongation axis as seen in control plants. Whole plants exposed to the magnetic field at any field intensity tested (5000-18,000 Gauss) displayed inhibited elongation growth. After two hours in a 5000 G field, plants show some recovery of elongation growth while maintained in the field, with elongation rates of 0.2 + 0.06 mm/hr as compared to control plants with 0.9 + 0.12 mm/hr elongation. Avena seeds which were germinated directly between pole pieces of a 5000 Gauss magnet produced slow-growing seedlings whose roots and coleoptiles grew toward opposite magnetic poles. Audus (1960) coined the phrase magnetotropism to describe a similar phenomenon in pollen tubes growing in magnetic fields.

Brown, D.S.; Gretz, M.; Brown, R.M. Jr.



Graduate Council Meeting Minutes 310 Jack K. Williams Administration Building  

E-print Network

. INTA 648 Contemporary Civil Wars i. INTA 672 East Asian Security j. INTA 673 Chinese Domestic Politics. INTA 676 International Politics of the Middle East n. INTA 687 Domestic Intelligence Operations


Levels of job satisfaction and role perceptions held by extension agents of INTA'S Regional Parana in Argentina  

E-print Network

organizations can change the work situation to enable employees to satisfy their needs through improved job performance. Two content models will be discussed: Herzberg's motivational-hygiene model and the needs hierarchy model developed by Maslow (22...) and adapted by McGregor (23) for use in personnel management. The content model is based on five need levels, which are physiological, safety, social, ego, and self-actualization. The model suggests that: l. Adult motives are highly complex. No single...

Blaha, Francisco Jose



[Mechanical myocardial support systems 1997: an overview of inta-aortic balloon counterpulsation to implantable left ventricular support systems].  


The development of cardiopulmonary bypass (CPB) to support the systemic circulation during cardiac surgical procedures became a clinical reality in 1953. Although the use of CPB for the treatment of post-infarction cardiogenic shock met with little success, intra-aortic balloon counterpulsation was used successfully in 1968 to reduce ischaemic injury in a patient with cardiogenic shock. Today, a broad spectrum of circulatory assist devices for short- and long-term application is available. Three major indication groups for the use of support devices are established. In low-cardiac-output syndrome after cardiac surgical procedures, short-term devices are utilised to enable myocardial recovery. In transplantation candidates suffering from drug-resistant pump failure, the implantation of long-term devices as a bridge to heart transplantation is indicated, and in NYHA class IV patients with contraindications to heart transplantation, the implantation of long-term devices as an alternative to transplantation is under discussion. In the literature, post-cardiotomy support survival is less than 30% on average. About 70% of mechanically bridged patients have survived to undergo heart transplantation and were transplanted with over 90% survival. Major problems during mechanical support are infection, bleeding, and thromboembolism. In view of patients' natural course without support, these clinical results are favourable. PMID:9245211

Hammel, D; Möllhoff, T; Soepawata, R; van Aken, H; Scheld, H H



IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions  

Microsoft Academic Search

Motivation: During the last few years, several new small regulatory RNAs (sRNAs) have been discovered in bacteria. Most of them act as post-transcriptional regulators by base pairing to a target mRNA, causing translational repression or activation, or mRNA degradation. Numerous sRNAs have already been identified, but the number of experimentally verified targets is considerably lower. Consequently, computational target prediction is

Anke Busch; Andreas S. Richter; Rolf Backofen



Water relations in Azospirillum -inoculated wheat seedlings under osmotic stress  

Microsoft Academic Search

Azospirillum has been shown to improve coleoptile growth in seedlings growing in darkness under osmotic stress. However, the changes in water relations that may occur in this experimental system have not yet been studied. Two-centimetre long Triticum aestivum cv. Buck PucarÆ and Triticum durum cv. Balcarceæo-INTA seedlings were inoculated with viable or autoclaved (control) Azospirillum brasilense Sp. 245 bacteria, at

Cecilia M. Creus; Rolando J. Sueldo; Carlos A. Barassi



Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice  

PubMed Central

The cardiac voltage-gated sodium channel, Nav1.5, plays a central role in cardiac excitability and impulse propagation and associates with the dystrophin multiprotein complex at the lateral membrane of cardiomyocytes. It was previously shown that Nav1.5 protein content and the sodium current (lNa) were both decreased in cardiomyocytes of dystrophin-deficient mdx5cv mice. In this study, wild-type and mdx5cv mice were treated for 7 days with the proteasome inhibitor MG132 (10 ?g/Kg/24 h) using implanted osmotic mini pumps. MG132 rescued both the total amount of Nav1.5 protein and lNa but, unlike in previous studies, de novo expression of dystrophin was not observed in skeletal or cardiac muscle. This study suggests that the reduced expression of Nav1.5 in dystrophin-deficient cells is dependent on proteasomal degradation. PMID:23532763

Rougier, Jean-Sebastien; Gavillet, Bruno; Abriel, Hugues



Regulation of the expression of GA-insensitive gene homolog by light in Oryza sativa c.v. DongJin.  


To understand the mechanism by which light regulates a gibberellin (GA)-insensitive gene in DongJinByeo (Oryza sativa cv DongJin), both green and etiolated DongJinByeo seedlings were submerged in water and treated with GA. Total RNA from the seedlings was isolated and hybridized with cDNA of a GA-insensitive gene homolog. The amount of transcript for the GA-insensitive gene homolog was higher in green seedlings than in etiolated seedlings in the absence of GA. However upon the addition of GA, greater accumulations of the gene transcript occurred in etiolated seedlings than in green seedlings. This result indicates the possibility that the expression of the GA-insensitive gene homolog transcript may be inhibited by light in the presence of GA. Light seems to regulate multilaterally the accumulation of the transcript of the GA-insensitive gene homolog in DongJinByeo (Oryza sativa cv DongJin). PMID:18831334

Kim, Jae Hong; Lee, Mi Young



Agrobacterium -Mediated Transformation of cry 1Ac Gene into Shoot-Tip Meristem of Diploid Cotton Gossypium arboreum cv. RG8 and Regeneration of Transgenic Plants  

Microsoft Academic Search

An Agrobacterium-mediated gene transfer protocol was developed for the diploid cotton Gossypium arboreum using meristematic cells of shoot tips, followed by direct shoot organogenesis or multiple shoot induction of putative transformants.\\u000a Seven-day- old shoot tips of in vitro-germinated seedlings of G. arboreum cv. RG8 were excised by removing cotyledonary leaves and providing “V”-shaped oblique cuts on either side of explants.

S. B. Nandeshwar; Sandhya Moghe; P. K. Chakrabarty; M. K. Deshattiwar; Keshav Kranthi; P. Anandkumar; C. D. Mayee; B. M. Khadi



Effect of exo-16,17-dihydro-gibberellin A5 on gibberellin A20 metabolism in seedlings of dwarf rice (Oryza sativa L. cv. Tan-ginbozu)  

Microsoft Academic Search

Several of the 16,17-dihydro gibberellins (GAs) inhibit elongation in a variety of species. In a study of their mechanism of action we have investigated the effect of exo-16,17-dihydro-Ga5 (diHGA5) on the metabolism of GA20 in dwarf rice (Oryza sativa cv. Tan-ginbozu). A mixture of [3H]- and [3H]-GA20 (100 ng per plant) was applied in microdrops to 4 d old seedlings

Makiko Takagi; David W. Pearce; Loeke M. Janzen; Richard P. Pharis



A chilling-insensitive stage in germination of a low-temperature-adapted radish, rat’s tail radish ( Raphanus sativus L.) cv. “Pakki-hood”  

Microsoft Academic Search

The rat’s tail radish (Raphanus sativus cv. “Pakki-hood”), cultivated in the northern part of Thailand, does not require low temperature for flower-bud initiation and shows high germinability even at low temperature. To analyze its characteristic temperature response, seedlings were subjected to a 3 day-chilling treatment at 0°C after a series of incubations for germination at 26°C which ranged from 3h

Kazunari Nomura; Ichitaro Endo; Akira Tateishi; Hiroaki Inoue; Kazuo Yoneda



Petrography, stable isotope compositions, microRaman spectroscopy, and presolar components of Roberts Massif 04133: A reduced CV3 carbonaceous chondrite  

NASA Astrophysics Data System (ADS)

Here, we report the mineralogy, petrography, C-N-O-stable isotope compositions, degree of disorder of organic matter, and abundances of presolar components of the chondrite Roberts Massif (RBT) 04133 using a coordinated, multitechnique approach. The results of this study are inconsistent with its initial classification as a Renazzo-like carbonaceous chondrite, and strongly support RBT 04133 being a brecciated, reduced petrologic type >3.3 Vigarano-like carbonaceous (CV) chondrite. RBT 04133 shows no evidence for aqueous alteration. However, it is mildly thermally altered (up to approximately 440 °C); which is apparent in its whole-rock C and N isotopic compositions, the degree of disorder of C in insoluble organic matter, low presolar grain abundances, minor element compositions of Fe,Ni metal, chromite compositions and morphologies, and the presence of unequilibrated silicates. Sulfides within type I chondrules from RBT 04133 appear to be pre-accretionary (i.e., did not form via aqueous alteration), providing further evidence that some sulfide minerals formed prior to accretion of the CV chondrite parent body. The thin section studied contains two reduced CV3 lithologies, one of which appears to be more thermally metamorphosed, indicating that RBT 04133, like several other CV chondrites, is a breccia and thus experienced impact processing. Linear foliation of chondrules was not observed implying that RBT 04133 did not experience high velocity impacts that could lead to extensive thermal metamorphism. Presolar silicates are still present in RBT 04133, although presolar SiC grain abundances are very low, indicating that the progressive destruction or modification of presolar SiC grains begins before presolar silicate grains are completely unidentifiable.

Davidson, Jemma; Schrader, Devin L.; Alexander, Conel M. O'd.; Lauretta, Dante S.; Busemann, Henner; Franchi, Ian A.; Greenwood, Richard C.; Connolly, Harold C.; Domanik, Kenneth J.; Verchovsky, Alexander



Zirconium immobilized on gold–mercaptopropionic acid self-assembled monolayer for trace determination of phosphate in blood serum by using CV, EIS, and OSWV  

Microsoft Academic Search

Preparation, characterization, and application of a new sensor for the determination of phosphate in blood serum is described by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and osteryoung square wave voltammetry (OSWV) in the presence of an appropriate redox reaction probe. The sensor was based on ionic adsorption of phosphate on Zr(IV) immobilized on gold–mercaptopropionic acid self-assembled monolayer (Au–MPA–Zr(IV) SAM)

Reza Karimi Shervedani; Sima Pourbeyram




E-print Network

arXiv:1001.5211v1[math.CV]28Jan2010 THE SEMIGROUP OF RIGGED ANNULI AND THE TEICHM¨ULLER SPACE to an element of this semigroup as a rigged annulus, where the term "rigging" refers to the boundary. Primary 30F60, 30C62, 58B12 ; Secondary 81T40. Key words and phrases. Semigroup of rigged annuli, Teichm

Radnell, David


Effects of water deficit on the vegetative response, yield and oil quality of olive trees ( Olea europaea L., cv Coratina) grown under intensive cultivation  

Microsoft Academic Search

An experiment was carried out in a young high-density olive grove (556plantsha?1—Olea europaea L., cv Coratina) located in Southern Italy to evaluate the effect of different soil water availability on the vegetative and productive performances of olive trees also looking into the quality of the resulting oils. Trials were carried out over a 3-year period on trees subjected to irrigation

Assunta Maria Palese; Vitale Nuzzo; Fabio Favati; Angiolina Pietrafesa; Giuseppe Celano; Cristos Xiloyannis



Distribution of resveratrol and stilbene synthase in young grape plants ( Vitis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C on its accumulation  

Microsoft Academic Search

Current research indicated that the resveratrol was mainly accumulated in the skin of grape berry, however, little is yet known about the distribution of resveratrol, as well as the regulation mechanism at protein level and the localization of stilbene synthase (malonyl-CoA:4-coumaroyl-CoA malonyltransferase; EC; STS), a key enzyme of resveratrol biosynthesis, in young grape plants (Vitis vinifera L. cv. Cabernet

Wei Wang; Ke Tang; Hao-Ru Yang; Peng-Fei Wen; Ping Zhang; Hui-Ling Wang; Wei-Dong Huang



Stress response proteins’ differential expression in embryogenic and non-embryogenic callus of Vitis vinifera L. cv. Cabernet Sauvignon—A proteomic approach  

Microsoft Academic Search

Co-cultivation of grape embryogenic callus (EC) and somatic embryos with Agrobacterium often leads to necrogenesis, which manifests in tissue browning and subsequent cell death. Interestingly, however, co-cultivation of grape non-embryogenic callus (NEC) does not display any visible symptoms. This paper compares the proteome of EC and NEC of Vitis vinifera L. cv. Cabernet Sauvignon to investigate the underlying mechanism. Analysis

Jiangwei Zhang; Huiqin Ma; Si Chen; Min Ji; Avihai Perl; Laszlo Kovacs; Shangwu Chen



Water–Gas Shift Reaction Over Aluminum Promoted Cu\\/CeO 2 Nanocatalysts Characterized by XRD, BET, TPR and Cyclic Voltammetry (CV)  

Microsoft Academic Search

A series of aluminum promoted Cu\\/CeO2 nanocatalysts with aluminum content in the range of 0–5wt.% were prepared by co-precipitation method and examined with respect\\u000a to their catalytic performance for the water–gas shift (WGS) reaction. The catalysts were characterized by XRD, BET, H2-TPR and cyclic voltammetry (CV) techniques. The results indicate that catalytic activity increases with the aluminum content\\u000a at first,

Lei Li; Yingying Zhan; Qi Zheng; Yuanhui Zheng; Xingyi Lin; Dalin Li; Junjiang Zhu



Production of plants resistant to Alternaria carthami via organogenesis and somatic embryogenesis of safflower cv. NARI-6 treated with fungal culture filtrates  

Microsoft Academic Search

The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower\\u000a (Carthamus\\u000a tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium\\u000a with different levels of FCF (10–50%) produced embryogenic callus.

J. Vijaya Kumar; B. D. Ranjitha Kumari; G. Sujatha; Enrique Castaño



The Study of Stem Recutting and Aluminum-sulphate Application on the Some Morphophysiologic Traits in Rosa (Rosa hybrida cv.illona)  

Microsoft Academic Search

This reasearch was conducted in agricultural faculty of Tehran University in 2005 for evaluation the effect of application aluminum-sulphate levels in preservation solution with stem recutting on longevity of rosa hybrida cv.Illona cut flower.Cut stems of rosa that produced in greenhouse from biennial mother stocks was placed under treatment of aluminum-sulphate solution with concentrations (100- 150- 200 ppm) and stem

S. N. Mortazavi; R. Naderi; M. Moradi Gharakhloo


Case3:11-cv-05310-EMC Document74-4 Filed05/09/12 Page2 of 22 (12) United States Patent  

E-print Network

Case3:11-cv-05310-EMC Document74-4 Filed05/09/12 Page2 of 22 (12) United States Patent Mikula (54, TX (US) Assignee: Symantec Operating Corporation, Mountain View, CA (US) ( *) Notice: Subject to any.: 101774,039 (22) Filed: Feb. 6, 2004 (51) Int. Cl. G06F 15/177 (2006.01) (52) U.S. Cl

Shamos, Michael I.


Cryopreservation of in vitro-cultured multiple bud clusters of asparagus ( Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification  

Microsoft Academic Search

A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated “bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to -196°C.

H. Kohmura; A. Sakai; S. Chokyu; T. Yakuwa



Formation and development of embryo and endosperm in intra- and inter-specific crosses of Asparagus officinalis and A. densiflorus cv. Sprengeri  

Microsoft Academic Search

Garden asparagus, Asparagus officinalis L. (2n=2x=20) is reproductively isolated from the related ornamental species Asparagus densiflorus (Kunth) Jessop cv. Sprengeri (2n=6x=60) by incompatibility barriers at the pollen–stigma and pollen–style levels, plus stronger post-stylar barriers, which have not been described, in pollen–pistil-compatible genotypes. To study the latter, intra- and inter-specific crosses were made using 25 plants of A. officinalis and 29

O. N Marcellán; E. L Camadro



Identification of novel low M r glutenin subunits in the high quality bread wheat cv Salmone and their effects on gluten quality  

Microsoft Academic Search

Southern-blot hybridization with a probe specific for genes encoding for low Mr glutenin subunits showed that the high quality bread wheat cv Salmone contains two DNA fragments designated as SF720 and SF750. These fragments were found to occur on the chromosome-1B satellite, and to be associated with the presence of two strongly staining low Mr glutenin subunits in the two-dimensional

P. Vaccino; R. Redaelli; E. Metakovsky; B. Borghi; M. Corbellini; N. Pogna



The C—V and G/?—V Electrical Characteristics of 60Co ?-Ray Irradiated Al/Si3N4/p-Si (MIS) Structures  

NASA Astrophysics Data System (ADS)

The influence of 60Co (?-ray) irradiation on the electrical characteristics of Al/Si3N4/p-Si (MIS) structures is investigated using capacitance-voltage (C—V) and conductance-voltage (G/?—V) measurements. The MIS structures are exposed to a 60Co ? radiation source at a dose of 0.7 kGy/h, with a total dose range of 0-100 kGy. The C—V and G/?—V properties are measured before and after 60Co ?-ray irradiation at 500 kHz and room temperature. It is found that the capacitance and conductance values decrease with the increase in the total dose due to the irradiation-induced defects at the interface. The results also indicate that ? radiation causes an increase in the barrier height ?B, Fermi energy EF and depletion layer width WD. The interface state density (Nss), using the Hill-Coleman method and dependent on radiation dose, is determined from the C—V and G/?—V measurements and decreases with an increase in the radiation dose. The decrease in the interface states can be attributed to the decrease in the recombination centers and the passivation of the Si surface due to the deposition insulator layer (Si3N4). In addition, it is clear that the acceptor concentration NA decreases with increasing radiation dose. The profile of series resistance Rs for various radiation doses is obtained from forward and reverse-biased C—V and G/?—V measurements, and its values decrease with increasing radiation dose, while it increases with increasing voltage in the accumulation region

Zeyrek, S.; Turan, A.; M. Bülbül, M.



Physical Characterization of the Potentially Hazardous Asteroid and Planetary Radar Target 68216 (2001 CV26): An Excellent Shape/Pole Modeling Candidate.  

NASA Astrophysics Data System (ADS)

The Near-Earth Asteroid (NEA) 68216 (2001 CV26) was discovered February 1 2001 by the LINEAR NEA Discovery Survey (MPEC 2001-F15). With an Absolute Magnitude H=16.2 and a Minimum Orbit Intersection Distance MOID=0.023 AU, it has been flagged as a Potentially Hazardous Asteroid (PHA) by the Minor Planet Center. The asteroid's 2009-2010 apparition allowed for radar observation at both the Arecibo and Goldstone facilities in October 2009.

Hicks, M.; Lawrence, K.; Somers, J.; Foster, J.



ICP OES and CV AAS in determination of mercury in an unusual fatal case of long-term exposure to elemental mercury in a teenager.  


In this work, a case of deliberate self-poisoning is presented. A 14-year-old girl suddenly died during one of the several hospitalizations. Abdominal computer tomography showed a large number of metallic particles in the large intestine. Analysis of blood and internal organs for mercury and other toxic metals carried out by inductively coupled plasma optical emission spectrometry (ICP OES) revealed high concentrations of mercury in kidneys and liver (64,200 and 2470ng/g, respectively), less in stomach (90ng/g), and none in blood. Using cold vapor-atomic absorption spectrometry (CV AAS), high levels of mercury were confirmed in all examined materials, including blood (87ng/g), and additionally in hair. The results of analysis obtained by two techniques revealed that the exposure to mercury was considerable (some time later, it was stated that the mercury originated from thermometers that had been broken over the course of about 1 year, because of Münchausen syndrome). CV AAS is a more sensitive technique, particularly for blood samples (negative results using ICP OES), and tissue samples - with LOQ: 0.63ng/g of Hg (CV AAS) vis-à-vis 70ng/g of Hg (ICP OES). However, ICP OES may be used as a screening technique for autopsy material in acute poisoning by a heavy metal, even one as volatile as mercury. PMID:24630410

Lech, Teresa



Hydrothermal origin of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A CAI from the Northwest Africa 2086 CV3 chondrite  

NASA Astrophysics Data System (ADS)

We report an occurrence of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A calcium-aluminum-rich inclusion (CAI) from the CV3 (Vigarano-like) carbonaceous chondrite Northwest Africa 2086. Dmisteinbergite occurs as approximately 10 ?m long and few micrometer-thick lath-shaped crystal aggregates in altered parts of the CAI, and is associated with secondary nepheline, sodalite, Ti-poor Al-diopside, grossular, and Fe-rich spinel. Spinel is the only primary CAI mineral that retained its original O-isotope composition (?17O ~ -24‰); ?17O values of melilite, perovskite, and Al,Ti-diopside range from -3 to -11‰, suggesting postcrystallization isotope exchange. Dmisteinbergite, anorthite, Ti-poor Al-diopside, and ferroan olivine have 16O-poor compositions (?17O ~ -3‰). We infer that dmisteinbergite, together with the other secondary minerals, formed by replacement of melilite as a result of fluid-assisted thermal metamorphism experienced by the CV chondrite parent asteroid. Based on the textural appearance of dmisteinbergite in NWA 2086 and petrographic observations of altered CAIs from the Allende meteorite, we suggest that dmisteinbergite is a common secondary mineral in CAIs from the oxidized Allende-like CV3 chondrites that has been previously misidentified as a secondary anorthite.

Fintor, Krisztian; Park, Changkun; Nagy, Szabolcs; Pál-Molnár, Elemér; Krot, Alexander N.



Comparative analysis of the energy levels of planar and core-twisted perylene bisimides in solution and solid state by UV/VIS, CV, and UPS/IPES  

NASA Astrophysics Data System (ADS)

The frontier orbital energies of four different functionalized perylene bisimide derivatives, PBI-Ph(iPr)2, PBI-H4, PBI-F2 and PBI-Cl4, were directly determined by UV-photo electron spectroscopy (UPS) and inverse photo electron spectroscopy (IPES) and are compared to the results from cyclic voltammetry (CV) and optical absorption spectroscopy (UV/VIS). The optical spectra reveal significant differences between monomeric species in solution and assembled molecules in the condensed state for the nearly planar PBI-H4 and PBI-F2, which are attributed to significant ?- ? stacking interactions in the condensed phase. In contrast, for PBIs with bulky substituents or twisted core, i.e. 2,6-isopropylphenyl substituents at the imide positions (PBI-Ph(iPr)2) or four chlorine substituents at perylene bay positions (PBI-Cl4), similar spectra are observed in solution and in the condensed state, which suggests the absence of strong intermolecular ?- ? stacking interactions. An entirely different result is obtained for the HOMO/LUMO energy values obtained from UPS/IPES and CV measurements which do not reveal a significant impact of intermolecular ?- ? stacking interactions. When comparing CV and UPS/IPES results, an accentuated deviation was observed for the perylene bisimide derivatives as compared to correlations found in the literature.

Arantes, C.; Scholz, M.; Schmidt, R.; Dehm, V.; Rocco, M. L. M.; Schöll, A.; Reinert, F.; Würthner, F.



A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata  

PubMed Central

Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays comprising 14?500 probes were used to compare, under three experimental conditions (in vitro, in the greenhouse, and in the vineyard), foliar material of infected symptomatic plants (S+R+), infected asymptomatic plants (S–R+), and healthy plants (S–R–). These plants were characterized by symptom notation after natural (vineyard) or experimental (in vitro and greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative real-time PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (Erysiphe necator, Plasmopara viticola, and Botrytis cinerea). (i) Five functional categories of genes, that is those involved in metabolism, defence reactions, interaction with the environment, transport, and transcription, were up-regulated in S+R+ plants compared with S–R– plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also up-regulated after infection by powdery mildew, downy mildew, and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and the polypeptide fraction secreted by Eutypa. PMID:20190040

Camps, Celine; Kappel, Christian; Lecomte, Pascal; Leon, Celine; Gomes, Eric; Coutos-Thevenot, Pierre; Delrot, Serge



Olivine-rich rims surrounding chondrules in the Mokoia CV3 carbonaceous chondrite: Further evidence for parent-body processes  

NASA Astrophysics Data System (ADS)

Fine-grained rims surrounding chondrules and inclusions in the Mokoia CV3 carbonaceous chondrite can be divided into phyllosilicate-rich and olivine-rich types. We present a petrographic and electron microscopic study of the olivine-rich rims and their host objects (referred to as chondrules/olivine-rich rims). The olivine-rich rims consist mainly of Fe-rich olivine and very minor phyllosilicate (saponite). Their host chondrules contain minor saponite and phlogopite, which resulted from aqueous alteration of anhydrous silicates. Mineralogical and compositional characteristics of the chondrules/olivine-rich rims suggest that they experienced mild thermal metamorphic effects. The rims commonly contain veins of coarse-grained Fe-rich olivine, magnetite, and Fe-(Ni) sulfides. The chondrules show abundant evidence of alteration along their peripheries, and the alteration textures suggest a mechanism for rim formation by replacement of the chondrules. Initially, enstatite and opaque nodules preferentially reacted to form coarse, platy, Fe-rich olivine crystals, which were subsequently divided into finer grains. Forsterite was also replaced by Fe-rich olivine. As the alteration advanced, these Fe-rich olivines were disaggregated, mixed with simultaneously produced saponite, and formed rims. In contrast, the surrounding matrix shows no evidence of such alteration and metamorphism. These observations indicate that the chondrules/olivine-rich rims did not experience these secondary processes in their present setting. The results suggest that the chondrules/olivine-rich rims experienced extensive replacement reactions in an environment in which aqueous fluids existed but only in minor amounts. They have probably also undergone simultaneous and/or subsequent mild thermal metamorphism. We suggest that the chondrules/olivine-rich rims are actually clasts transported from a relatively dry region in the parent body that was different from the region where Mokoia was finally lithified.

Tomeoka, Kazushige; Ohnishi, Ichiro



Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor  

PubMed Central

Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in relation to short and long term periods of cold followed by a brief frost treatment were studied in order to disclose proteins responsible for the cold hardening process in distinct plant tissues. The mentioned changes have been monitored using two dimensional difference gel electrophoresis (2D-DIGE) with subsequent peptide-mapping protein identification. Regarding approximately 600–700 distinct protein spots detected on 2D gels, there has been found at least a two-fold change after exposure to low temperatures in about 10% of proteins in leaves and 13% of proteins in crowns. Protein and nitrogen metabolic processes have been influenced by low temperature to a similar extent in both tissues while catabolism, carbohydrate metabolism and proteins involved in stress response have been more affected in crowns than in leaves. The range of changes in protein abundance was generally higher in leaves and chloroplast proteins were frequently affected which suggests a priority to protect photosynthetic apparatus. Overall, our data proved existence of slightly different response strategies to low temperature stress in crowns and leaves, i.e., tissues with different biological role. Moreover, there have been found several proteins with large increase in accumulation, e.g., 33 kDa oxygen evolving protein of photosystem II in leaves and “enhanced disease susceptibility 1” in crowns; these proteins might have potential to indicate an enhanced level of frost tolerance in barley. PMID:23584021

Hlavackova, Iva; Vitamvas, Pavel; Santrucek, Jiri; Kosova, Klara; Zelenkova, Sylva; Prasil, Ilja Tom; Ovesna, Jaroslava; Hynek, Radovan; Kodicek, Milan



Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor.  


Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in relation to short and long term periods of cold followed by a brief frost treatment were studied in order to disclose proteins responsible for the cold hardening process in distinct plant tissues. The mentioned changes have been monitored using two dimensional difference gel electrophoresis (2D-DIGE) with subsequent peptide-mapping protein identification. Regarding approximately 600-700 distinct protein spots detected on 2D gels, there has been found at least a two-fold change after exposure to low temperatures in about 10% of proteins in leaves and 13% of proteins in crowns. Protein and nitrogen metabolic processes have been influenced by low temperature to a similar extent in both tissues while catabolism, carbohydrate metabolism and proteins involved in stress response have been more affected in crowns than in leaves. The range of changes in protein abundance was generally higher in leaves and chloroplast proteins were frequently affected which suggests a priority to protect photosynthetic apparatus. Overall, our data proved existence of slightly different response strategies to low temperature stress in crowns and leaves, i.e., tissues with different biological role. Moreover, there have been found several proteins with large increase in accumulation, e.g., 33 kDa oxygen evolving protein of photosystem II in leaves and "enhanced disease susceptibility 1" in crowns; these proteins might have potential to indicate an enhanced level of frost tolerance in barley. PMID:23584021

Hlavá?ková, Iva; Vítámvás, Pavel; Santr??ek, Ji?í; Kosová, Klára; Zelenková, Sylva; Prášil, Ilja Tom; Ovesná, Jaroslava; Hynek, Radovan; Kodí?ek, Milan



A Lectin with Highly Potent Inhibitory Activity toward Breast Cancer Cells from Edible Tubers of Dioscorea opposita cv. Nagaimo  

PubMed Central

A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC50 values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

Chan, Yau Sang; Ng, Tzi Bun



Root exudation and root development of lettuce (Lactuca sativa L. cv. Tizian) as affected by different soils  

PubMed Central

Development and activity of plant roots exhibit high adaptive variability. Although it is well-documented, that physicochemical soil properties can strongly influence root morphology and root exudation, particularly under field conditions, a comparative assessment is complicated by the impact of additional factors, such as climate and cropping history. To overcome these limitations, in this study, field soils originating from an unique experimental plot system with three different soil types, which were stored at the same field site for 10 years and exposed to the same agricultural management practice, were used for an investigation on effects of soil type on root development and root exudation. Lettuce (Lactuca sativa L. cv. Tizian) was grown as a model plant under controlled environmental conditions in a minirhizotrone system equipped with root observation windows (rhizoboxes). Root exudates were collected by placing sorption filters onto the root surface followed by subsequent extraction and GC-MS profiling of the trapped compounds. Surprisingly, even in absence of external stress factors with known impact on root exudation, such as pH extremes, water and nutrient limitations/toxicities or soil structure effects (use of sieved soils), root growth characteristics (root length, fine root development) as well as profiles of root exudates were strongly influenced by the soil type used for plant cultivation. The results coincided well with differences in rhizosphere bacterial communities, detected in field-grown lettuce plants cultivated on the same soils (Schreiter et al., this issue). The findings suggest that the observed differences may be the result of plant interactions with the soil-specific microbiomes. PMID:24478764

Neumann, G.; Bott, S.; Ohler, M. A.; Mock, H.-P.; Lippmann, R.; Grosch, R.; Smalla, K.



Anaerobic 1-Alkene Metabolism by the Alkane- and Alkene-Degrading Sulfate Reducer Desulfatibacillum aliphaticivorans Strain CV2803T?  

PubMed Central

The alkane- and alkene-degrading, marine sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T, known to oxidize n-alkanes anaerobically by fumarate addition at C-2, was investigated for its 1-alkene metabolism. The total cellular fatty acids of this strain were predominantly C-(even number) (C-even) when it was grown on C-even 1-alkenes and predominantly C-(odd number) (C-odd) when it was grown on C-odd 1-alkenes. Detailed analyses of those fatty acids by gas chromatography-mass spectrometry after 6- to 10-week incubations allowed the identification of saturated 2- and 4-ethyl-, 2- and 4-methyl-, and monounsaturated 4-methyl-branched fatty acids with chain lengths that correlated with those of the 1-alkene. The growth of D. aliphaticivorans on (per)deuterated 1-alkenes provided direct evidence of the anaerobic transformation of these alkenes into the corresponding 1-alcohols and into linear as well as 10- and 4-methyl-branched fatty acids. Experiments performed with [13C]bicarbonate indicated that the initial activation of 1-alkene by the addition of inorganic carbon does not occur. These results demonstrate that D. aliphaticivorans metabolizes 1-alkene by the oxidation of the double bond at C-1 and by the subterminal addition of organic carbon at both ends of the molecule [C-2 and C-(?-1)]. The detection of ethyl-branched fatty acids from unlabeled 1-alkenes further suggests that carbon addition also occurs at C-3. Alkylsuccinates were not observed as potential initial intermediates in alkene metabolism. Based on our observations, the first pathways for anaerobic 1-alkene metabolism in an anaerobic bacterium are proposed. Those pathways indicate that diverse initial reactions of 1-alkene activation can occur simultaneously in the same strain of sulfate-reducing bacterium. PMID:17965214

Grossi, Vincent; Cravo-Laureau, Cristiana; Meou, Alain; Raphel, Danielle; Garzino, Frederic; Hirschler-Rea, Agnes



Anaerobic 1-alkene metabolism by the alkane- and alkene-degrading sulfate reducer Desulfatibacillum aliphaticivorans strain CV2803T.  


The alkane- and alkene-degrading, marine sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803(T), known to oxidize n-alkanes anaerobically by fumarate addition at C-2, was investigated for its 1-alkene metabolism. The total cellular fatty acids of this strain were predominantly C-(even number) (C-even) when it was grown on C-even 1-alkenes and predominantly C-(odd number) (C-odd) when it was grown on C-odd 1-alkenes. Detailed analyses of those fatty acids by gas chromatography-mass spectrometry after 6- to 10-week incubations allowed the identification of saturated 2- and 4-ethyl-, 2- and 4-methyl-, and monounsaturated 4-methyl-branched fatty acids with chain lengths that correlated with those of the 1-alkene. The growth of D. aliphaticivorans on (per)deuterated 1-alkenes provided direct evidence of the anaerobic transformation of these alkenes into the corresponding 1-alcohols and into linear as well as 10- and 4-methyl-branched fatty acids. Experiments performed with [(13)C]bicarbonate indicated that the initial activation of 1-alkene by the addition of inorganic carbon does not occur. These results demonstrate that D. aliphaticivorans metabolizes 1-alkene by the oxidation of the double bond at C-1 and by the subterminal addition of organic carbon at both ends of the molecule [C-2 and C-(omega-1)]. The detection of ethyl-branched fatty acids from unlabeled 1-alkenes further suggests that carbon addition also occurs at C-3. Alkylsuccinates were not observed as potential initial intermediates in alkene metabolism. Based on our observations, the first pathways for anaerobic 1-alkene metabolism in an anaerobic bacterium are proposed. Those pathways indicate that diverse initial reactions of 1-alkene activation can occur simultaneously in the same strain of sulfate-reducing bacterium. PMID:17965214

Grossi, Vincent; Cravo-Laureau, Cristiana; Méou, Alain; Raphel, Danielle; Garzino, Frédéric; Hirschler-Réa, Agnès



Root exudation and root development of lettuce (Lactuca sativa L. cv. Tizian) as affected by different soils.  


Development and activity of plant roots exhibit high adaptive variability. Although it is well-documented, that physicochemical soil properties can strongly influence root morphology and root exudation, particularly under field conditions, a comparative assessment is complicated by the impact of additional factors, such as climate and cropping history. To overcome these limitations, in this study, field soils originating from an unique experimental plot system with three different soil types, which were stored at the same field site for 10 years and exposed to the same agricultural management practice, were used for an investigation on effects of soil type on root development and root exudation. Lettuce (Lactuca sativa L. cv. Tizian) was grown as a model plant under controlled environmental conditions in a minirhizotrone system equipped with root observation windows (rhizoboxes). Root exudates were collected by placing sorption filters onto the root surface followed by subsequent extraction and GC-MS profiling of the trapped compounds. Surprisingly, even in absence of external stress factors with known impact on root exudation, such as pH extremes, water and nutrient limitations/toxicities or soil structure effects (use of sieved soils), root growth characteristics (root length, fine root development) as well as profiles of root exudates were strongly influenced by the soil type used for plant cultivation. The results coincided well with differences in rhizosphere bacterial communities, detected in field-grown lettuce plants cultivated on the same soils (Schreiter et al., this issue). The findings suggest that the observed differences may be the result of plant interactions with the soil-specific microbiomes. PMID:24478764

Neumann, G; Bott, S; Ohler, M A; Mock, H-P; Lippmann, R; Grosch, R; Smalla, K



Gelation in protein extracts from cold acclimated and non-acclimated winter rye (Secale cereale L. cv Musketeer).  


A protein gel is a three-dimensional network consisting of molecular interactions between biopolymers that entrap a significant volume of a continuous liquid phase (water). Molecular interactions in gels occur at junction zones within and between protein molecules through electrostatic forces, hydrogen bonding, hydrophobic associations (van der Waals attractions) and covalent bonding. Gels have the physicochemical properties of both solids and liquids, and are extremely important in the production and stability of a variety of foods, bioproducts and pharmaceuticals. In this study, gelation was induced in phenol extracted protein fractions from non-acclimated (NA) and cold-acclimated (CA) winter rye (Secale cereale L. cv Musketeer) leaf tissue after repeated freeze-thaw treatments. Gel formation only occurred at high pH (pH 12.0) and a minimum of 3-4 freeze-thaw cycles were required. The gel was thermally stable and only a specific combination of chemical treatments could disrupt the gel network. SDS-PAGE analysis identified ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) as the major protein component in the gel, although Rubisco itself did not appear to be a factor in gelation. Raman spectroscopy suggested changes in protein secondary structure during freeze-thaw cycles. Overall, the NA and CA gels were similar in composition and structure, with the exception that the CA gel appeared to be amyloidic in nature based on thioflavin T (ThT) fluorescence. Protein gelation, particularly in the apoplast, may confer protection against freeze-induced dehydration and potentially have a commercial application to improve frozen food quality. PMID:23348601

Lim, Ze Long; Low, Nicholas H; Moffatt, Barbara A; Gray, Gordon R



Quantified, whole section trace element mapping of carbonaceous chondrites by Synchrotron X-ray Fluorescence Microscopy: 1. CV meteorites  

NASA Astrophysics Data System (ADS)

We present the application of a new synchrotron-based technique for rapid mapping of trace element distributions across large areas of the CV3 meteorites Allende and Vigarano. This technique utilizes the Australian Synchrotron X-ray Fluorescence Microscopy (XFM) beam line with its custom designed and built X-ray detector array called Maia. XFM with Maia allows data to be collected using a 2 ?m spot size at very low dwell times (?0.1-0.5 ms), resulting in maps of entire thin sections in ?5 h. Maia is an energy dispersive detector system with a large collection solid-angle, which allows full spectral acquisition and high sensitivity. Hence, there is no need to constrain the elements of interest a priori. We collected whole section maps (?2 cm × 1 cm) from 3 thick sections of Allende and a single map (2 cm × 1.5 cm) from a thick section of Vigarano. Our experimental conditions provide data for elements with 20 ? Z ? 40 (K-shell, Ca through Zr) and the L-emissions of Os, Ir, Pt, Au, and Pb. We illustrate the unique capabilities of this technique by presenting observations across myriad length scales, from the centimeter-scale down to the detection of sub-micrometer particles within these objects. Our initial results show the potential of this technique to help decipher spatial and textural variations in trace element chemistry between CAIs, chondrules, matrix, and other chondritic components. We also illustrate how these datasets can be applied to understanding both nebular and parent-body processes within meteorites.

Dyl, Kathryn A.; Cleverley, James S.; Bland, Phil A.; Ryan, Chris G.; Fisher, Louise A.; Hough, Robert M.



FANTINA: Fathom Asteroids Now: Tomography and Imagery of a NEA- Payload For Marco Polo R CV3 / ESA mission  

NASA Astrophysics Data System (ADS)

The internal structure of small bodies is still poorly known and has never been measured directly. There is no way to determine from ground based observation whether the body is a monolithic piece of rock or a rubble-pile, an aggregate of boulders held together by gravity and how much porosity it contains, both in the form of micro-scale or macro-scale porosity. Knowing this structure is a key point for a better understanding of the asteroid accretion and dynamical evolution. It is the main objective of the FANTINA experience proposed Payload for Marco Polo R CV3 / ESA mission: FANTINA (Marco Polo's Daughter) for Fathom Asteroids Now: Tomography and Imagery of a NEA is to provide measurement capabilities and science data which are not accessible by remote sensing of the asteroid from the Marco-Polo R spacecraft alone and which complement the analysis of the returned samples. The FANTINA payload is a Lander carrying the ASSERT experiment and a complementary payload to be defined: ASSERT is a bistatic CONSERT/Rosetta-like radar to achieve the tomography both in transmission and in reflexion of the asteroid in order to characterise its composition and its heterogeneity from decimetric to global scale. The lander is a long-lived bus in the 10-kg class derived from MASCOT/Hayabusa 2. This paper reviews the science rationale of FANTINA in the context of the Marco Polo R mission to 1996FG3. The surface package concept will be reviewed including the radar tomography principles. So a preliminary design and budget will be done.

Herique, A.; Biele, J.; Bousquet, P.; Ciarletti, V.; Ho, T. M.; Issler, J. L.; Kofman, W.; Michel, P.; Plettemeier, D.; Puget, P.; Souyris, J. C.; Ulamec, S.; van Zoest, T.; Zine, S.



PhDAHP1 is required for floral volatile benzenoid/phenylpropanoid biosynthesis in Petunia × hybrida cv 'Mitchell Diploid'.  


Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis consists of numerous enzymatic and regulatory processes. The initial enzymatic step bridging primary metabolism to secondary metabolism is the condensation of phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) carried out via 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE (DAHP) synthase. Here, identified, cloned, localized, and functionally characterized were two DAHP synthases from the model plant species Petunia × hybrida cv 'Mitchell Diploid' (MD). Full-length transcript sequences for PhDAHP1 and PhDAHP2 were identified and cloned using cDNA SMART libraries constructed from pooled MD corolla and leaf total RNA. Predicted amino acid sequence of PhDAHP1 and PhDAHP2 proteins were 76% and 80% identical to AtDAHP1 and AtDAHP2 from Arabidopsis, respectively. PhDAHP1 transcript accumulated to relatively highest levels in petal limb and tube tissues, while PhDAHP2 accumulated to highest levels in leaf and stem tissues. Through floral development, PhDAHP1 transcript accumulated to highest levels during open flower stages, and PhDAHP2 transcript remained constitutive throughout. Radiolabeled PhDAHP1 and PhDAHP2 proteins localized to plastids, however, PhDAHP2 localization appeared less efficient. PhDAHP1 RNAi knockdown petunia lines were reduced in total FVBP emission compared to MD, while PhDAHP2 RNAi lines emitted 'wildtype' FVBP levels. These results demonstrate that PhDAHP1 is the principal DAHP synthase protein responsible for the coupling of metabolites from primary metabolism to secondary metabolism, and the ultimate biosynthesis of FVBPs in the MD flower. PMID:24815009

Langer, Kelly M; Jones, Correy R; Jaworski, Elizabeth A; Rushing, Gabrielle V; Kim, Joo Young; Clark, David G; Colquhoun, Thomas A



Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pera.  


Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant. PMID:17609534

Purcino, Rúbia P; Medina, Camilo Lázaro; Martins de Souza, Daniel; Winck, Flávia Vischi; Machado, Eduardo Caruso; Novello, José Camilo; Machado, Marcos Antonio; Mazzafera, Paulo



A lectin with highly potent inhibitory activity toward breast cancer cells from edible tubers of Dioscorea opposita cv. nagaimo.  


A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC(50) values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

Chan, Yau Sang; Ng, Tzi Bun



John Cushman's CV  

E-print Network

SIAM Multiscale Modeling and Simulation, 4(4): 1233-1244. Park* ... Mixture theory for three-scale biocompatible polymers and a computational example. .... theory of reactive transport with physical, chemical and biological heterogeneity. ..... ARO Symposium on Science and Complex Adaptive Systems , Durham NC (

F. Alejandro Bonilla


Kenney cv short  

E-print Network

*Kenney, R.H. & *de Oliveira, L. (In Press) A framework for connecting natural .... Evidence of involvement in the graduate research program of the department. a. .... Mathematics Education Masters Program - Helped define a new program that

Rachael Kenney



Growth, Pigment Synthesis, and Ultrastructural Responses of Phaseolus vulgaris L. cv. Blue Lake to Intermittent and Flashing Light 12  

PubMed Central

Growing bean plants (Phaseolus vulgaris L. cv. Blue Lake) on cycles of 1 minute light-1 minute dark or 5 minutes light-5 minutes dark, providing an integrated 12 hours light-12 hours dark per day for each set of plants, led to production after 21 days of new leaves low or lacking in chloroplast pigments. Subsequently, dry weight increase was sharply cut. Leaf area was affected by the light regimes after the second week of growth. By the fourth week, plants on the 1 minute light-1 minute dark cycle showed about one-half the leaf area of the controls. Shoot growth was favored over root growth to the greatest degree on the 1 minute light-1 minute dark regimes. Chlorophyll a/b ratios were close to 3.0 in all of the intermittent light regimes, but the total amounts of chlorophyll in milligrams per primary leaf were higher from day 9 to day 23 for the 12 hour light-12 hours dark controls than for other plants. Although they produced chlorophyll, the plants receiving 1 or 2 milliseconds per second of light continued to lose weight at the same rate as the dark controls; thus, it is assumed there was no net photosynthesis. Plants receiving flashing light allocated significantly more food reserves from the seed to roots than did dark controls. Total chlorophyll formation was significantly accelerated by 2 milliseconds per second light. With 1 millisecond per second light, it took 5 days longer to achieve the same level of chlorophyll. After the 18th day, there was a steady decline in chlorophyll, b degrading more rapidly than a. It is thought that several light-driven reactions are involved in the observed pigment synthesis, photosynthesis, food allocation, and growth of bean. Some of these reactions may be cyclic and others linear. Collectively, they must reach a harmonic point for normal metabolism and development to occur. Because time courses for each of these reactions are different, the intermittent and flashing light technique offers the possibility of individually studying some of the key light-driven reactions. Images Fig. 8 Fig. 9 PMID:16662457

Naylor, Aubrey W.; Giles, Lawrence J.



Efficacy of Pneumococcal Nontypable Haemophilus influenzae Protein D Conjugate Vaccine (PHiD-CV) in Young Latin American Children: A Double-Blind Randomized Controlled Trial  

PubMed Central

Background The relationship between pneumococcal conjugate vaccine–induced antibody responses and protection against community-acquired pneumonia (CAP) and acute otitis media (AOM) is unclear. This study assessed the impact of the ten-valent pneumococcal nontypable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) on these end points. The primary objective was to demonstrate vaccine efficacy (VE) in a per-protocol analysis against likely bacterial CAP (B-CAP: radiologically confirmed CAP with alveolar consolidation/pleural effusion on chest X-ray, or non-alveolar infiltrates and C-reactive protein ? 40 µg/ml); other protocol-specified outcomes were also assessed. Methods and Findings This phase III double-blind randomized controlled study was conducted between 28 June 2007 and 28 July 2011 in Argentine, Panamanian, and Colombian populations with good access to health care. Approximately 24,000 infants received PHiD-CV or hepatitis control vaccine (hepatitis B for primary vaccination, hepatitis A at booster) at 2, 4, 6, and 15–18 mo of age. Interim analysis of the primary end point was planned when 535 first B-CAP episodes, occurring ?2 wk after dose 3, were identified in the per-protocol cohort. After a mean follow-up of 23 mo (PHiD-CV, n?=?10,295; control, n?=?10,201), per-protocol VE was 22.0% (95% CI: 7.7, 34.2; one-sided p?=?0.002) against B-CAP (conclusive for primary objective) and 25.7% (95% CI: 8.4%, 39.6%) against World Health Organization–defined consolidated CAP. Intent-to-treat VE was 18.2% (95% CI: 5.5%, 29.1%) against B-CAP and 23.4% (95% CI: 8.8%, 35.7%) against consolidated CAP. End-of-study per-protocol analyses were performed after a mean follow-up of 28–30 mo for CAP and invasive pneumococcal disease (IPD) (PHiD-CV, n?=?10,211; control, n?=?10,140) and AOM (n?=?3,010 and 2,979, respectively). Per-protocol VE was 16.1% (95% CI: ?1.1%, 30.4%; one-sided p?=?0.032) against clinically confirmed AOM, 67.1% (95% CI: 17.0%, 86.9%) against vaccine serotype clinically confirmed AOM, 100% (95% CI: 74.3%, 100%) against vaccine serotype IPD, and 65.0% (95% CI: 11.1%, 86.2%) against any IPD. Results were consistent between intent-to-treat and per-protocol analyses. Serious adverse events were reported for 21.5% (95% CI: 20.7%, 22.2%) and 22.6% (95% CI: 21.9%, 23.4%) of PHiD-CV and control recipients, respectively. There were 19 deaths (n?=?11,798; 0.16%) in the PHiD-CV group and 26 deaths (n?=?11,799; 0.22%) in the control group. A significant study limitation was the lower than expected number of captured AOM cases. Conclusions Efficacy was demonstrated against a broad range of pneumococcal diseases commonly encountered in young children in clinical practice. Trial registration NCT00466947 Please see later in the article for the Editors' Summary PMID:24892763

Tregnaghi, Miguel W.; Saez-Llorens, Xavier; Lopez, Pio; Abate, Hector; Smith, Enrique; Posleman, Adriana; Calvo, Arlene; Wong, Digna; Cortes-Barbosa, Carlos; Ceballos, Ana; Tregnaghi, Marcelo; Sierra, Alexandra; Rodriguez, Mirna; Troitino, Marisol; Carabajal, Carlos; Falaschi, Andrea; Leandro, Ana; Castrejon, Maria Mercedes; Lepetic, Alejandro; Lommel, Patricia; Hausdorff, William P.; Borys, Dorota; Guinazu, Javier Ruiz; Ortega-Barria, Eduardo; Yarzabal, Juan P.; Schuerman, Lode



MOST: A Powerful Tool to Reveal the True Nature of the Mysterious Dust-Forming Wolf-Rayet Binary CV Ser  

NASA Astrophysics Data System (ADS)

The WR + O binary CV Ser has been a source of mystery since it was shown that its atmospheric eclipses change with time over decades, in addition to its sporadic dust production. However, the first high-precision time-dependent photometric observations obtained with the MOST space telescope in 2009 show two consecutive eclipses over the 29 day orbit, with varying depths. A subsequent MOST run in 2010 showed a somewhat asymmetric eclipse profile. Parallel optical spectroscopy was obtained from the Observatoire du Mont-Mégantic (2009 and 2010) and from the Dominion Astrophysical Observatory (2009).

David-Uraz, A.; Moffat, A. F. J.; Chené, A.-N.; MOST Collaboration



Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John's wort (Hypericum perforatum L. cv. Anthos) plants  

Microsoft Academic Search

Hypericum perforatum   cv. Anthos) is presented. Isotope tracer experiments were performed on plantlets regenerated from thidiazuron-induced stem\\u000a explants and grown on MS basal medium for 2 months. Radiolabel from 14C-tryptophan was recovered as 14C-indoleacetic acid, 14C-tryptamine, 14C-5-hydroxytryptophan, 14C-serotonin and 14C-melatonin in the treated St. John's wort plantlets. Chromatographic peak identity was confirmed by high performance liquid\\u000a chromatography-mass spectrometry-mass spectrometry and

S. J. Murch; S. KrishnaRaj; P. K. Saxena



Understanding the atmospheric structure of T Tauri stars - II. UV spectroscopy of RY Tau, BP Tau, RU Lupi, GW Ori and CV Cha  

NASA Astrophysics Data System (ADS)

We report results from our study of International Ultraviolet Explorer (IUE) data of a group of T Tauri stars (TTS). Comparisons between UV-line fluxes in these stars and in the Sun indicate very high levels of activity in their atmospheres and comparatively higher electron densities. Spectroscopic diagnostic line ratios indicate densities over an order of magnitude higher than in the `quiet' Sun at `transition region' temperatures. At these densities, metastable levels can attain comparable populations to the ground level and ionization fractions can be altered as a result of the sensitivity of dielectronic recombination. In Brooks et al. we improved the treatment of these effects using the adas software package, the atomic models and data of which are based on collisional-radiative theory. Here we extend the analysis to a sample of five TTS: RY Tau, BP Tau, RU Lupi, GW Ori and CV Cha. Using these models and data we derive the emission measure (EM) distribution for each star in the sample. We find that the decrease in EM with increasing temperature appears to be sharper than that found in previous work. In comparison with the Sun, the results suggest that the UV emission is formed in a region with a steeper density or volume gradient. We find mismatches between the theoretical and observed fluxes which cannot be explained by density effects and thus must be a result of uncertainties in the atomic data, unreliabilities in the fluxes or the failure of physical assumptions in the method. We have made a series of tests and comparisons, including examination of opacity effects, and these clearly favour the latter explanation. They also lead us to suggest the presence of two separate components in the UV emission for each of the TTS, although the case of CV Cha is more ambiguous. This supports and extends the earlier work of Jordan & Kuin on RU Lupi. Interestingly, we find that the EM distribution for GW Ori has values at least 10 times larger than those of RY Tau, which appears to have approximately the same electron density. A similar difference is found between CV Cha and RU Lupi. Following geometrical arguments, we suggest that the UV emission in GW Ori and CV Cha is formed in a more extended region than in the other three stars.

Brooks, D. H.; Costa, V. M.; Lago, M. T. V. T.; Lanzafame, A. C.



[Establishment of embryogenic cell suspension culture and plant regeneration of edible banana Musa acuminata cv. Mas (AA)].  


Conventional breeding for dual resistance of disease and pest of Musa cultivars remains a difficult endeavor, as the plant is polyploidic and high in sterility. Biotechnological techniques, eg., genetic engineering, in vitro mutation breeding, or protoplast fusion, may overcome the difficulties and improve the germplasm. Establishment of a stable embryogenic cell suspension (ECS) is a prerequisite for any of the biotechnological breeding methods. In this study an embryogenic cell suspension was established from immature male flower of Musa acuminata cv. Mas (AA), a popular commercial variety of banana in the South-East Asian region. After culture for 5-6 months on callus induction media, which consisted of MS salts, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 4.1 micromol/L biotin, 5.7 micromol/L indoleacetic acid (IAA), 5.4 micromol/L naphthaleneacetic acid (NAA), other vitamins, 87 mmol/L sucrose, and solidified with 7 g/L agarose, meristematic globules and yellow, friable embryogenic cultures were induced from the explants of 1-15th row young floral hands of immature male flowers. Of the four treatments of 2,4-D, 9 micromol/L was the most effective on the callus induction, it transformed 40.96% and 7.45% of the cultivated male floral hands into callus and embryogenic callus respectively. The explants to produce highest frequency of the embryogenic calli were floral hands of 6 to 12th rows, which generated 5.79% of the embryogenic calli. Suspension cultures were initiated from these embryogenic calli in liquid medium supplemented with 4.5 micromol/L 2, 4-D. After sieving selection of the cultures using a stainless steel metallic strainer with pore sizes of 154 microm at 15 day intervals for 3 months, homogeneous and yellow embryogenic cell suspensions, composed of single cells and small cell aggregates, were established. Based upon the growth quantity and growth rate of ECS, it was determined that the appropriate inoculum was 2.0 mL PCV ECS/30 mL medium in 100 mL flask, and the appropriate subculture cycle was 15 days. Planting of 6 months old ECS on semi-solid medium of somatic embryo induction and development (MSD) resulted in approximately 280 x 10(3) somatic embryos/mL PCV ECS. MSD contained SH macronutrients, micro-nutrients, Fe-EDTA and MS vitamins supplemented with 4.5 micromol/L biotin, 680 micromol/L glutamine, 2 mmol/L proline, 100 mg/L malt extract, 1.1 micromol/L NAA, 0.2 micromol/L zeatin, 0.5 micromol/L kinetin, 0.7 micromol/L N6-(2-isopentenyl) adenine, 29 mmol/L lactose, 130 mmol/L sucrose and solidified with 2g/L gelrite. After 3 months of maturity on MSD, 17.28% of the somatic embryos were germinated on germination media (MG), consisted of MS salt, Morel and Wetmore vitamins, 0.2 micromol/L 6-BA, 1.1 micromol/L IAA, 87 micromol/L sucrose and solidified with 2 g/L gelrite; and 14.16% of the somatic embryos could develop into normal plantlets on rooting media contained the same composition as that of MG but without auxin and cytokinin. PMID:15859330

Wei, Yue-Rong; Huang, Xue-Lin; Li, Jia; Huang, Xia; Li, Zhe; Li, Xiao-Ju



Multilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv 'Fortune'  

PubMed Central

The genetic structure of 2n gametes and, particularly, the parental heterozygosity restitution at each locus depends on the meiotic process by which they originated, with first-division restitution and second-division restitution (SDR) being the two major mechanisms. The origin of 2n gametes in citrus is still controversial, although sexual polyploidisation is widely used for triploid seedless cultivar development. In this study, we report the analysis of 2n gametes of mandarin cv ‘Fortune' by genotyping 171 triploid hybrids with 35 simple sequence repeat markers. The microsatellite DNA allele counting-peak ratios method for allele-dosage evaluation proved highly efficient in segregating triploid progenies and allowed half-tetrad analysis (HTA) by inferring the 2n gamete allelic configuration. All 2n gametes arose from the female genitor. The observed maternal heterozygosity restitution varied between 10 and 82%, depending on the locus, thus SDR appears to be the mechanism underlying 2n gamete production in mandarin cv ‘Fortune'. A new method to locate the centromere, based on the best fit between observed heterozygosity restitution within a linkage group and theoretical functions under either partial or no chiasmata interference hypotheses was successfully applied to linkage group II. The maximum value of heterozygosity restitution and the pattern of restitution along this linkage group would suggest there is partial chiasma interference. The implications of such a restitution mechanism for citrus breeding are discussed. PMID:21587302

Cuenca, J; Froelicher, Y; Aleza, P; Juarez, J; Navarro, L; Ollitrault, P



S-asteroids 387 Aquitania and 980 Anacostia - Possible fragments of the breakup of a spinel-bearing parent body with CO3/CV3 affinities  

NASA Technical Reports Server (NTRS)

Asteroids 387 Aquitania and 980 Anacostia are anomalous members of the S-class. Their reflectance spectra exhibit a strong broad absorption feature longwards of 1.5 micron and no significant feature near 1 micron. Their spectra indicate the presence of spinel, an aluminum-magnesium oxide mineral commonly present in inclusions in CV3 and CO3 meteorites. Spinel probably makes up only a small percentage of the surface assemblages of these asteroids, but its spectral effect may be enhanced by its presence in fine-grained white inclusions in immature asteroid regoliths. It is speculated that Aquitania and Anacostia represent material formed in the same nebular zone as the CV3 and CO3 chondrites but either: (A) at an earlier time in the nebula when such inclusions might have been a relatively larger fraction of the nebular grain population; or (B) in local regions where nebular processes (e.g., settling to the midplane) had concentrated such inclusions. The close similarity of two orbital elements (a, i) suggests that Aquitania and Anacostia may be members of a partially dispersed asteroid family produced by the early disruption of a spinel-bearing parent body.

Burbine, Thomas H.; Gaffey, Michael J.; Bell, Jeffrey F.



3D BrainCV: simultaneous visualization and analysis of cells and capillaries in a whole mouse brain with one-micron voxel resolution.  


Systematic cellular and vascular configurations are essential for understanding fundamental brain anatomy and metabolism. We demonstrated a 3D brainwide cellular and vascular (called 3D BrainCV) visualization and quantitative protocol for a whole mouse brain. We developed a modified Nissl staining method that quickly labeled the cells and blood vessels simultaneously in an entire mouse brain. Terabytes 3D datasets of the whole mouse brains, with unprecedented details of both individual cells and blood vessels, including capillaries, were simultaneously imaged at 1-?m voxel resolution using micro-optical sectioning tomography (MOST). For quantitative analysis, we proposed an automatic image-processing pipeline to perform brainwide vectorization and analysis of cells and blood vessels. Six representative brain regions from the cortex to the deep, including FrA, M1, PMBSF, V1, striatum, and amygdala, and six parameters, including cell number density, vascular length density, fractional vascular volume, distance from the cells to the nearest microvessel, microvascular length density, and fractional microvascular volume, had been quantitatively analyzed. The results showed that the proximity of cells to blood vessels was linearly correlated with vascular length density, rather than the cell number density. The 3D BrainCV made overall snapshots of the detailed picture of the whole brain architecture, which could be beneficial for the state comparison of the developing and diseased brain. PMID:24185025

Wu, Jingpeng; He, Yong; Yang, Zhongqin; Guo, Congdi; Luo, Qingming; Zhou, Wei; Chen, Shangbin; Li, Anan; Xiong, Benyi; Jiang, Tao; Gong, Hui



Safety and immunogenicity of 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Nigerian children: Booster dose and 2-dose catch-up regimens in the second year of life.  


In a previous study, 3-dose primary vaccination of Nigerian infants with the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) was immunogenic for vaccine pneumococcal serotypes, with comparable tolerability between PHiD-CV and control groups. In an open-label study (, NCT01153893), 68 primed children received a PHiD-CV booster dose co-administered with a diphtheria-tetanus-acellular pertussis (DTPa) booster dose at 15-21 months and 36 children unprimed for pneumococcal vaccination received two PHiD-CV catch-up doses (first dose co-administered with DTPa booster dose) at 15-21 and 17-23 months. Adverse events were recorded and immune responses were measured before and one month after vaccination. In both groups, pain was the most frequent solicited local symptom and fever was the most frequent solicited general symptom after the booster dose and each catch-up dose. Few grade 3 solicited symptoms and no vaccine-related serious adverse events were reported. After booster vaccination, for each vaccine serotype, at least 98.5% of children had an antibody concentration ? 0.2 µg/ml and at least 94.0% had an opsonophagocytic activity (OPA) titer ? 8. After 2-dose catch-up, for each vaccine serotype, at least 97.1% had an antibody concentration ? 0.2 µg/ml, except for serotypes 6B (82.9%) and 23F (88.6%), and at least 91.4% had an OPA titer ?8, except for serotypes 6B (77.4%) and 19F (85.3%). PHiD-CV induced antibody responses against protein D in both groups. In conclusion, PHiD-CV administered to Nigerian toddlers as a booster dose or 2-dose catch-up was well tolerated and immunogenic for vaccine pneumococcal serotypes and protein D. PMID:24356787

Odusanya, Olumuyiwa O; Kuyinu, Yetunde A; Kehinde, Omolara A; Shafi, Fakrudeen; François, Nancy; Yarzabal, Juan Pablo; Dobbelaere, Kurt; Rüggeberg, Jens U; Borys, Dorota; Schuerman, Lode



Distribution of GA20-13-O-glucoside and reconversion of [17-D2]GA20-13-O-glucoside in caryopses of Hordeum vulgare L. cv. Himalaya  

Microsoft Academic Search

The distribution of GA20 and GA20-13-O-glucoside was examined in mature caryopses of Hordeum vulgare L. cv. Himalaya. It was shown by GC-MS that GA20-13-O-glucoside is localized mainly in the embryo-containing part of the grain. In order to study the reconversion of GA20-13-O-glucoside during early germination, the glucoside pool of cv. Himalaya was [17-D2]-labelled by feeding [17-D2]GA20. The metabolites of [17-D2]GA20-13-O-glucoside

B. Senns; P. Fuchs; G. Schneider



Primary vaccination with the 10-valent pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in infants in Mali and Nigeria: a randomized controlled trial  

PubMed Central

Background Pneumonia is still the leading cause of death among children in Africa, and pneumococcal serotypes 1 and 5 are frequently isolated from African children with invasive pneumococcal disease below the age of 5 years. The immunogenicity, safety and reactogenicity of 3-dose primary vaccination with the 10-valent pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) were evaluated in infants in Mali and Nigeria. Methods In an open, randomized, controlled study, 357 infants received DTPw-HBV/Hib and OPV primary vaccination with (PHiD-CV group) or without (control group) PHiD-CV co-administration at 6, 10 and 14 weeks of age. Pneumococcal antibody responses and opsonophagocytic activity (OPA) were measured and adverse events (AEs) recorded. Results One month post-dose 3, ? 97.2% of PHiD-CV-vaccinated infants had an antibody concentration ? 0.2 ?g/mL for each vaccine pneumococcal serotype except for 6B (82.0%) and 23F (87.6%) versus < 10% in the control group except for serotypes 14 (35.7%) and 19F (22.5%). For each vaccine serotype, ? 93.3% of PHiD-CV recipients had an OPA titre ? 8, except for serotypes 1 (87.6%) and 6B (85.4%), compared to < 10% in the control group, except for serotypes 7F (42.9%), 9V (24.1%) and 14 (24.5%). Anti-protein D geometric mean antibody concentrations were 3791.8 and 85.4 EL.U/mL in the PHiD-CV and control groups, respectively. Overall incidences of solicited and unsolicited AEs were similar between groups. Conclusions In sub-Saharan African infants, PHiD-CV was immunogenic for all vaccine pneumococcal serotypes and protein D. Vaccine tolerability was generally comparable between the PHiD-CV and control groups. Trial Registration identifier: NCT00678301. PMID:22112189



On the use of Ethephon as abscising agent in cv. Crimson Seedless table grape production: Combination of Fruit Detachment Force, Fruit Drop and metabolomics.  


The effect of 2-chloroethylphosphonic acid (Ethephon, in the following ETH) as abscising agent on cv. Crimson Seedless table grape was investigated by means of Fruit Detachment Force (FDF) and Fruit Drop (FD) analyses combined with a metabolomic study carried out by High Resolution Mass Spectrometry (HRMS) and Nuclear Magnetic Resonance (NMR) spectroscopy. The effectiveness of ETH as abscising agent was ascertained with ETH concentration ranging from 1.4 to 4.0g/L in a two-year study. The ETH treatments caused berry drops higher than 40% and induced an increase of tartaric acid, procyanidin P2, terpenoid derivatives and peonidin-3-glucoside as well as a decrease of catechin and epicatechin. HRMS-NMR covariance analysis was carried out to correlate the fluctuations of tartaric acid NMR signals to those of MS peaks of the secondary metabolites affected by ETH treatments. PMID:25308678

Rizzuti, Antonino; Aguilera-Sáez, Luis Manuel; Gallo, Vito; Cafagna, Isabella; Mastrorilli, Piero; Latronico, Mario; Pacifico, Andrea; Matarrese, Angela Maria Stella; Ferrara, Giuseppe



Crystalline xylitol production by a novel yeast, Pichia caribbica (HQ222812), and its application for quorum sensing inhibition in gram-negative marker strain Chromobacterium violaceum CV026.  


Xylitol, a sugar alcohol, is fast gaining ground over other artificial sugar substitutes owing to its advantageous properties. Xylitol is a safer alternative for diabetics because of insulin-independent metabolism. It has beneficial properties suitable to form an important part of odontological formulations. Conventional commercial production of xylitol involves harsh chemical method operating at high temperature and pressure. Thus, microbial production of xylitol is preferred over chemical method, and yeasts have been extensively exploited for this purpose. In the present manuscript, quantitative production of xylitol from D-xylose with the yield of 0.852 gm/gm and volumetric productivity of 1.83 gm/l/h in crystalline form, using novel yeast Pichia caribbica is reported. Also, a mild, safe procedure for product extraction is described. The ability of xylitol to act as a quorum sensing antagonist in gram-negative marker strain Chromobacterium violaceum CV026 has been demonstrated for the first time. PMID:23338824

Mukherji, Ruchira; Joshi-Navare, Kasturi; Prabhune, Asmita



Light Microscopy Study of Nodule Initiation in Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5) 1  

PubMed Central

We compared nodule initiation in lateral roots of Pisum sativum (L.) cv Sparkle and in a low-nodulating mutant E2 (sym 5). In Sparkle, about 25% of the infections terminated in the epidermis, a similar number stopped in the cortex, and 50% resulted in the formation of a nodule meristem or an emerged nodule. The mutant E2 (sym 5) was infected as often as was the parent, and it formed a normal infection thread. In the mutant, cell divisions rarely occurred in advance of the infection thread, and few nodule primordia were produced. Growing the mutant at a low root temperature or adding Ag+ to the substrate increased the number of cell divisions and nodule primordia. We conclude that, in the E2 line, the infection process is arrested in the cortex, at the stage of initial cell divisions before the establishment of a nodule primordium. ImagesFigure 1 PMID:16668510

Guinel, Frederique C.; LaRue, Thomas A.



A refractory inclusion in the Kaba CV3 chondrite - Some implications for the origin of spinel-rich objects in chondrites  

NASA Technical Reports Server (NTRS)

The first detailed petrographic and mineralogical study of a Ca, Al-rich inclusion (CAI) from the Kaba CV3 chondrite is reported. This 'fine-grained' CAI contains abundant small, rounded, rimmed, spinel-rich objects which have important features in common with the spinel-rich objects in other carbonaceous and ordinary chondrites. These nodules are interpreted as fractionated distillation residues of primitive dust. However, the available data do not unambiguously rule out a condensation origin for at least some of these objects. Finally, the preservation of distinct diopside-hedenbergite rims on the spinel-rich bodies and the small grain size of many minerals in the CAI matrix material both suggest that the CAI accreted cool and had a relatively cool thermal history in the Kaba parent body.

Fegley, B., Jr.; Post, J. E.



Studies on the management of root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex of green gram, Vigna radiata cv ML-1108.  


Studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared to untreated inoculated plants. Analysis of data showed that carbofuran and A. indica seed powder increased plant growth and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens. PMID:16052706

Haseeb, Akhtar; Sharma, Anita; Shukla, Prabhat Kumar



Studies on the management of root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex of green gram, Vigna radiata cv ML-1108  

PubMed Central

Studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita–wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared to untreated inoculated plants. Analysis of data showed that carbofuran and A. indica seed powder increased plant growth and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens. PMID:16052706

Haseeb, Akhtar; Sharma, Anita; Shukla, Prabhat Kuma



Determination of replacement of some inorganic elements in pulvinus of bean (Phaseolus vulgaris cv. Gina 2004) at chilling temperature by the WDXRF spectroscopic technique  

NASA Astrophysics Data System (ADS)

In this study, bean seedlings (Phaseolus vulgaris cv. Gina 2004) were exposed to chilling temperatures until leaves are wrinkled (9 day), that is, showed nyctinastic movement. Pulvinus were subsequently were cut from the leaves. Concentrations of inorganic elements (P, S, Cl, K, Ca, Cu) in the pulvinus were measured by wavelength-dispersive X-ray fluorescence (WDXRF) spectrometry. Results indicated that concentration change (%) was not significant for Ca (0.82) but it was significant for K, P, Cl, S, and especially Cu concentrations (5.4%, 12.8%, 40.2%, 43.7%, 365%, respectively) in pulvinus of plants exposed to chilling temperature compared with control group. We hypothesize here the presence of association between nyctinasti movement brought about by pulvinus at chilling temperature in bean and changes of K, P, Cl, S and especially Cu concentrations measured by WDXRF analysis method.

Dumlupinar, Rahmi; Demir, Faruk; Budak, Gokhan; Karabulut, Abdulhalik; Kadi, Nuray; Karakurt, Halil; Erdal, Serkan



Identification of flavonol and xanthone glycosides from mango (Mangifera indica L. Cv. "Tommy Atkins") peels by high-performance liquid chromatography-electrospray ionization mass spectrometry.  


Flavonol O- and xanthone C-glycosides were extracted from mango (Mangifera indica L. cv. "Tommy Atkins") peels and characterized by high-performance liquid chromatography-electrospray ionization mass spectrometry. Among the fourteen compounds analyzed, seven quercetin O-glycosides, one kaempferol O-glycoside, and four xanthone C-glycosides were found. On the basis of their fragmentation pattern, the latter were identified as mangiferin and isomangiferin and their respective galloyl derivatives. A flavonol hexoside with m/z 477 was tentatively identified as a rhamnetin glycoside, which to the best of our knowledge, has not yet been reported in mango peels. The results obtained in the present study confirm that peels originating from mango fruit processing are a promising source of phenolic compounds that might be recovered and used as natural antioxidants or functional food ingredients. PMID:12903961

Schieber, Andreas; Berardini, Nicolai; Carle, Reinhold



2012 Virginia Polytechnic Institute and State University CV-22NP Virginia Cooperative Extension programs and employment are open to all, regardless of race, color, national origin, sex, religion, age, disability, political beliefs, sexual orientation, or  

E-print Network

2012 Virginia Polytechnic Institute and State University CV-22NP Virginia Cooperative Extension and State University, Virginia State University, and the U.S. Department of Agriculture cooperating. Edwin J, Administrator, 1890 Extension Program, Virginia State, Petersburg. Community Engagement: Successes in Virginia

Liskiewicz, Maciej


Introducing constricted variational density functional theory in its relaxed self-consistent formulation (RSCF-CV-DFT) as an alternative to adiabatic time dependent density functional theory for studies of charge transfer transitions  

NASA Astrophysics Data System (ADS)

We have applied the relaxed and self-consistent extension of constricted variational density functional theory (RSCF-CV-DFT) for the calculation of the lowest charge transfer transitions in the molecular complex X-TCNE between X = benzene and TCNE = tetracyanoethylene. Use was made of functionals with a fixed fraction (?) of Hartree-Fock exchange ranging from ? = 0 to ? = 0.5 as well as functionals with a long range correction (LC) that introduces Hartree-Fock exchange for longer inter-electronic distances. A detailed comparison and analysis is given for each functional between the performance of RSCF-CV-DFT and adiabatic time-dependent density functional theory (TDDFT) within the Tamm-Dancoff approximation. It is shown that in this particular case, all functionals afford the same reasonable agreement with experiment for RSCF-CV-DFT whereas only the LC-functionals afford a fair agreement with experiment using TDDFT. We have in addition calculated the CT transition energy for X-TCNE with X = toluene, o-xylene, and naphthalene employing the same functionals as for X = benzene. It is shown that the calculated charge transfer excitation energies are in as good agreement with experiment as those obtained from highly optimized LC-functionals using adiabatic TDDFT. We finally discuss the relation between the optimization of length separation parameters and orbital relaxation in the RSCF-CV-DFT scheme.

Krykunov, Mykhaylo; Seth, Mike; Ziegler, Tom



Smoking cessation and the risk of cardiovascular disease outcomes predicted from established risk scores: Results of the Cardiovascular Risk Assessment among Smokers in Primary Care in Europe (CV-ASPIRE) Study  

PubMed Central

Background Smoking is a major risk factor for cardiovascular disease (CVD). This multicenter, cross-sectional survey was designed to estimate the cardiovascular (CV) risk attributable to smoking using risk assessment tools, to better understand patient behaviors and characteristics related to smoking, and characterize physician practice patterns. Methods 1,439 smokers were recruited from Europe during 2011. Smokers were ?40 years old, smoked > 10 cigarettes/day and had recent measurements on blood pressure and lipids. CV risk was calculated using the SCORE system, Framingham risk equations, and Progetto CUORE model. The CV risk attributable to smoking was evaluated using a simulated control (hypothetical non-smoker) with identical characteristics as the enrolled smoker. Risks assessed included CV mortality, coronary heart disease (CHD), CVD and hard CHD. Demographics, comorbidities, primary reasons for consultation, behavior towards previous attempts to quit, and interest in smoking cessation was assessed. Dependence on nicotine was evaluated using the Fagerström Test for Nicotine Dependence. GP practice patterns were assessed through a questionnaire. Results The prediction models consistently demonstrated a high CV risk attributable to smoking. For instance, the SCORE model demonstrated that this study population of smokers have a 100% increased probability of death due to cardiovascular disease in the next 10-years compared to non-smokers. A considerable amount of patients would like to hear from their GP about the different alternatives available to support their quitting attempt. Conclusions The findings of this study reinforce the importance of smoking as a significant predictor of long-term cardiovascular events. One of the best gains in health could be obtained by tackling the most important modifiable risk factors; these results suggest smoking is among the most important. PMID:23597191



Dietary actinidin from kiwifruit (Actinidia deliciosa cv. Hayward) increases gastric digestion and the gastric emptying rate of several dietary proteins in growing rats.  


Dietary actinidin influences the extent to which some dietary proteins are digested in the stomach, and it is hypothesized that the latter modulation will in turn affect their gastric emptying rate (GE). In this study, the effect of dietary actinidin on GE and gastric digestion of 6 dietary protein sources was determined in growing rats. Each dietary protein source [beef muscle, gelatin, gluten, soy protein isolate (SPI), whey protein isolate, and zein] was included in 2 semisynthetic diets as the sole nitrogen source. For each protein source, 1 of the 2 diets contained actinidin [76.5 U/g dry matter (DM)] in the form of ground freeze-dried green kiwifruit (Actinidia deliciosa cv. Hayward), whereas the other diet contained freeze-dried gold kiwifruit (Actinidia chinensis cv. Hort16A), which is devoid of actinidin (3.4 U/g DM). For both diets, dietary kiwifruit represented 20% of the diet on a DM basis. The real-time GE was determined in rats gavaged with a single dose of the diets using magnetic resonance spectroscopy over 150 min (n = 8 per diet). Gastric protein digestion was determined based on the free amino groups in the stomach chyme collected from rats fed the diets (n = 8 per diet) that were later killed. GE differed across the protein sources [e.g., the half gastric emptying time (T(½)) ranged from 157 min for gluten to 266 min for zein] (P < 0.05). Dietary actinidin increased the gastric digestion of beef muscle (0.6-fold), gluten (3.2-fold), and SPI (0.6-fold) and increased the GE of the diets containing beef muscle (43% T(½)) and zein (23% T(½); P < 0.05). There was an inverse correlation between gastric protein digestion and DM retained in the stomach (r = -0.67; P < 0.05). In conclusion, dietary actinidin increased gastric protein digestion and accelerated the GE for several dietary protein sources. GE may be influenced by gastric protein digestion, and dietary actinidin can be used to modulate GE and protein digestion in the stomach of some dietary protein sources but not others. PMID:24431326

Montoya, Carlos A; Hindmarsh, Jason P; Gonzalez, Lucrecia; Boland, Mike J; Moughan, Paul J; Rutherfurd, Shane M



Characterization of the most odor-active volatiles of orange wine made from a Turkish cv. Kozan (Citrus sinensis L. Osbeck).  


The aroma-active compounds of cv. Turkish Kozan orange wine were analyzed by sensory and instrumental analyses. Liquid-liquid extraction with dichloromethane was used for extraction of volatile components. According to sensory analysis, the aromatic extract obtained by liquid-liquid extraction was representative of orange wine odor. A total of 63 compounds were identified and quantified in orange wine. The results of the gas chromatography-olfactometry analysis showed that 35 odorous compounds were detected by the panelists. Of these, 28 aroma-active compounds were identified. Alcohols followed by terpenes and esters were the most abundant aroma-active compounds of the orange wine. Among these compounds, ethyl butanoate (fruity sweet), 3-methyl-1-pentanol (roasty), linalool (floral citrusy), gamma-butyrolactone (cheesy burnt sugar), 3-(methylthio)-propanol (boiled potato, rubber), geraniol (floral citrusy), and 2-phenylethanol (floral rose) were the most important contributors to the aroma of the orange wine because they were perceived by all eight panelists. PMID:18078317

Selli, Serkan; Canbas, Ahmet; Varlet, Vincent; Kelebek, Hasim; Prost, Carole; Serot, Thierry



Senescence-specific alteration of hydrogen peroxide levels in Arabidopsis thaliana and oilseed rape spring variety Brassica napus L. cv. Mozart.  


In order to analyze the signaling function of hydrogen peroxide (H(2)O(2)) production in senescence in more detail, we manipulated intracellular H(2)O(2) levels in Arabidopsis thaliala (L.) Heynh by using the hydrogen-peroxide-sensitive part of the Escherichia coli transcription regulator OxyR, which was directed to the cytoplasm as well as into the peroxisomes. H(2)O(2) levels were lowered and senescence was delayed in both transgenic lines, but OxyR was found to be more effective in the cytoplasm. To transfer this knowledge to crop plants, we analyzed oilseed rape plants Brassica napus L. cv. Mozart for H(2)O(2) and its scavenging enzymes catalase (CAT) and ascorbate peroxidase (APX) during leaf and plant development. H(2)O(2) levels were found to increase during bolting and flowering time, but no increase could be observed in the very late stages of senescence. With increasing H(2)O(2) levels, CAT and APX activities declined, so it is likely that similar mechanisms are used in oilseed rape and Arabidopsis to control H(2)O(2) levels. Under elevated CO(2) conditions, oilseed rape senescence was accelerated and coincided with an earlier increase in H(2)O(2) levels, indicating that H(2)O(2) may be one of the signals to inducing senescence in a broader range of Brassicaceae. PMID:22805117

Bieker, Stefan; Riester, Lena; Stahl, Mark; Franzaring, Jürgen; Zentgraf, Ulrike



Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema x grandiflorum cv. "Mottenohoka" Is Enhanced by Suppressing Phosphorylation of p38MAPK  

PubMed Central

Components from Dendranthema × grandiflorum cv. “Mottenohoka” that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth. PMID:23554829

Kimura, Hirokazu; Tsukagoshi, Hiroyuki; Kozawa, Kunihisa; Koketsu, Mamoru; Ninomiya, Masayuki; Furukawa, Shoei



Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway.  


The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7 ?g/g dry weight (DW) and a maximum of 31.3 ?g/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3 ?g/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag. PMID:24530945

Ritala, Anneli; Dong, Lemeng; Imseng, Nicole; Seppänen-Laakso, Tuulikki; Vasilev, Nikolay; van der Krol, Sander; Rischer, Heiko; Maaheimo, Hannu; Virkki, Arho; Brändli, Johanna; Schillberg, Stefan; Eibl, Regine; Bouwmeester, Harro; Oksman-Caldentey, Kirsi-Marja



Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901.  


Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit. PMID:23615464

Zhu, Xiaoyan; Luo, Fenglei; Zheng, Yixiong; Zhang, Jiukai; Huang, Jianzhen; Sun, Chongde; Li, Xian; Chen, Kunsong



Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901  

PubMed Central

Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit. PMID:23615464

Zhu, Xiaoyan; Luo, Fenglei; Zheng, Yixiong; Zhang, Jiukai; Huang, Jianzhen; Sun, Chongde; Li, Xian; Chen, Kunsong



Seasonal changes in starch and sugar content of poplar (Populus deltoides x nigra cv. Dorskamp) and the impact of stem girdling on carbohydrate allocation to roots.  


Trees need to store reserves to allow their survival during winter and for bud flush and leaf growth in the following spring. In many tree species, these reserve functions are mainly covered by starch, which is degraded to soluble carbohydrates during the dormant season for maintenance respiration and in spring during bud flush. We conducted girdling experiments on poplar (Populus deltoides x nigra cv. Dorskamp) in order to elucidate how interrupted transport of carbohydrates to the roots during autumn affects plant survival during winter and bud flush in spring. We measured the content of starch, sucrose, glucose, fructose, raffinose and stachyose in stems (above and below the girdle), coarse roots and fine roots over 1 year. We found that, in response to girdling, carbohydrates accumulated in stems above the girdle. As a result of interrupted reserve allocation, girdled plants depleted their root starch reserves nearly to zero, whereas in stems below the girdle, reserves were maintained close to control values, presumably in order to facilitate dormancy release and re-sprouting from buds below the girdle. Furthermore, we showed that stachyose accumulated during winter also in the roots, even in girdled plants, consistent with its importance as freezing protectant. The lower stachyose content of roots compared with shoots was likely due to protection of the roots from cold by the soil. PMID:20538808

Regier, Nicole; Streb, Sebastian; Zeeman, Samuel C; Frey, Beat



Plant growth enhancing effects by a siderophore-producing endophytic streptomycete isolated from a Thai jasmine rice plant (Oryza sativa L. cv. KDML105).  


An endophytic Streptomyces sp. GMKU 3100 isolated from roots of a Thai jasmine rice plant (Oryza sativa L. cv. KDML105) showed the highest siderophore production on CAS agar while phosphate solubilization and IAA production were not detected. A mutant of Streptomyces sp. GMKU 3100 deficient in just one of the plant growth promoting traits, siderophore production, was generated by inactivation of a desD-like gene encoding a key enzyme controlling the final step of siderophore biosynthesis. Pot culture experiments revealed that rice and mungbean plants inoculated with the wild type gave the best enhancement of plant growth and significantly increased root and shoot biomass and lengths compared with untreated controls and siderophore-deficient mutant treatments. Application of the wild type in the presence or absence of ferric citrate significantly promoted plant growth of both plants. The siderophore-deficient mutant clearly showed the effect of this important trait involved in plant-microbe interaction in enhancement of growth in rice and mungbean plants supplied with sequestered iron. Our results highlight the value of a substantial understanding of the relationship of the plant growth promoting properties of endophytic actinomycetes to the plants. Endophytic actinomycetes, therefore, can be applied as potentially safe and environmentally friendly biofertilizers in agriculture. PMID:22836676

Rungin, Siriwan; Indananda, Chantra; Suttiviriya, Pavinee; Kruasuwan, Worarat; Jaemsaeng, Ratchaniwan; Thamchaipenet, Arinthip



The sequence of change within the photosynthetic apparatus of wheat following short-term exposure to ozone. [Triticum aestivum L. cv Avalon  

SciTech Connect

The basis of inhibition of photosynthesis by single acute O{sub 3} exposures was investigated in vivo using analyses based on leaf gas exchange measurements. The fully expanded second leaves of wheat plants (Triticum aestivum L. cv Avalon) were fumigated with either 200 or 400 nanomoles per mole O{sub 3} for between 4 and 16 hours. This reduced significantly the light-saturated rate of Co{sub 2} uptake and was accompanied by a parallel decrease in stomatal conductance. However, the stomatal limitation only increased significantly during the first 8 hours of exposure to 400 nanomoles per mole O{sub 3}; no significant increase occurred for any of the other treatments. Analysis of the response of CO{sub 2} uptake to the internal Co{sub 2} concentration implied that the predominant factor responsible for the reduction in light-saturated CO{sub 2} uptake was a decrease in the efficiency of carboxylation. At saturating concentrations of Co{sub 2}, photosynthesis was inhibited by no more than 22% after 16 hours, indicating that the capacity for regeneration of ribulose bisphosphate was less susceptible to O{sub 3}. Ozone fumigations also had a less pronounced effect on light-limited photosynthesis. The photochemical efficiency of photosystem II estimated from the ratio of variable to maximum chlorophyll fluorescence and the atrazine-binding capacity of isolated thylakoids demonstrated that photochemical reactions were not responsible for the initial inhibition of CO{sub 2} uptake.

Farage, P.K.; Long, S.P.; Baker, N.R. (Univ. of Essex (England)); Lechner, E.G. (Univ. of Vienna (Austria))



Callus initiation and plant regeneration from inflorescence primordia of the intergeneric hybrid Agropyron repens (L.) Beauv.xBromus inermis Leyss. cv. nanus on a modified nutritive medium.  


Plant regeneration from callus of intergeneric hybrid Agropyron repens (L.) Beauv. x Bromus inermis Leyss cv. nanus (AGROMUS) was carried out on a new culture medium designated medium-F. Within 21 days of the plating of inflorescence primordia the initiated callus showed globular structures. From the 21st day of culture, one step plant regeneration occurred on the callus without subculture. The new basal medium reported in this work was effective in callus initiation and plant regeneration of the hybrid AGROMUS by (i) the reduction of the total ion strength (2.6 g/l, 22.5 mM) of macroelements compared to MS (4.5 g/l,45.2 mM), (ii) the use of NH4NO3 as the sole N-source, and (iii) the application of KH2PO4 at an 8 times higher concentration (1160 mg/l,8.5 mM) when compared to the Murashige and Skoog medium composition. This medium provided a 2 to 10 fold reduction in the 2,4-dichlorophenoxyacetic acid supplement needed for the callus initiation and one step plant regeneration after a gibberellic acid (2 mg/l, for 5 days) pretreatment of tillers. The regenerated plantlets were subcultured in multi-shoot culture and potted in soil to grow for further analysis. PMID:24203137

Gyulai, G; Janovszky, J; Kiss, E; Lelik, L; Csillag, A; Heszky, L E



Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and desiccation in Spinacia oleracea L. cv. Bloomsdale: possible role in stress tolerance.  


Osmopriming improves seed germination performance as well as stress tolerance. To understand the biochemistry of osmopriming-induced seed stress tolerance, we investigated dehydrin (DHN) accumulation patterns at protein and transcript level (determined by immunoblotting and qPCR) during priming, and subsequent germination under optimal and stress conditions (i.e. chilling and desiccation) in spinach (Spinacia oleracea L. cv. Bloomsdale) seeds. Our data indicate enhanced germination performance of primed seeds is accompanied by increased accumulation of three dehydrin-like proteins (DLPs): 30, 26, and 19-kD. Moreover, 30, 26 and 19-kD DLPs that first only transiently accumulated during priming re-accumulated in response to stresses, suggesting an evidence for 'cross-tolerance', which is initially induced by priming and later recruited during post-priming germination under stresses. Study with CAP85, a spinach DHN, corroborates above observations at the gene-expression and protein accumulation level. Additionally, our results suggest that during seed germination and seedling establishment, CAP85 expression may be regulated by the interplay of two factors: seedling development and stress responses. In conclusion, our data suggest that 30, 26, and 19-kD dehydrin-like proteins and CAP85 may be used as potential biochemical/molecular markers for priming-induced stress tolerance in 'Bloomsdale' spinach. PMID:22195574

Chen, Keting; Fessehaie, Anania; Arora, Rajeev



The F-box protein COI1 functions upstream of MYB305 to regulate primary carbohydrate metabolism in tobacco (Nicotiana tabacum L. cv. TN90)  

PubMed Central

Jasmonate (JA) plays an important role in regulating plant male fertility and secondary metabolism, but its role in regulating primary metabolism remains unclear. The F-box protein CORONATINE INSENSITIVE 1 (COI1) is a critical component of the JA receptor, and mediates JA-signalling by targeting JASMONATE ZIM-domain (JAZ) proteins for proteasomal degradation in response to JA perception. Here, we found that RNA interference-mediated knockdown of NtCOI1 in tobacco (Nicotiana tabacum L. cv. TN90) recapitulated many previously observed phenotypes in coi1 mutants, including male sterility, JA insensitivity, and loss of floral anthocyanin production. It also affected starch metabolism in the pollen, anther wall, and floral nectary, leading to pollen abortion and loss of floral nectar. Transcript levels of genes encoding starch metabolism enzymes were significantly altered in the pollen, anther wall, and floral nectary of NtCOI1-silenced tobacco. Changes in leaf primary metabolism were also observed in the NtCOI1-silenced tobacco. The expression of NtMYB305, an orthologue of MYB305 previously identified as a flavonoid metabolic regulator in Antirrhinum majus flowers and as a floral-nectar regulator mediating starch synthesis in ornamental tobacco, was extremely downregulated in NtCOI1-silenced tobacco. These findings suggest that NtCOI1 functions upstream of NtMYB305 and plays a fundamental role in coordinating plant primary carbohydrate metabolism and correlative physiological processes. PMID:24604735

Zhang, Hongbo



The photosynthetic and stomatal response of Medicago sativa cv. saranac to free-air CO{sub 2} enrichment (F.A.C.E.) and nitrogen  

SciTech Connect

Plots of Medicago sativa cv. saranac were grown in the field at ambient (355 {mu}mol CO{sub 2} mol{sup -1} air) or elevated (600{mu}mol CO{sub 2} mol{sup -1} air) CO{sub 2} concentrations. High (200kg yr{sup -1}) or low (20kg yr{sup -1}) nitrogen levels were applied to two isogeneic lines, one able and one unable to use nitrogen fixing bacteria. Plants were in the second year of field growth. Exposure to elevated CO{sub 2} was via a Free-Air CO{sub 2} Enrichment System (FACE). Elevated CO{sub 2} increased diurnal assimilation by between 12% and 92%. Analysis of A/C{sub i} responses showed that effective nitrogen fertilisation was more important to rubisCO and RuBP activity than elevated CO{sub 2}. No acclimation was consistently observed. Leaves lower down the canopy were found to have lower Vc{sub max} and J{sub max} values, though age may be the cause of the latter effect. FACE conditions have only a small effect on these responses. There was some evidence found for the down-regulation of photosynthesis in the late afternoon. The FACE conditions had no affect on stomatal density but did increase epidermal cell density.

Bridson, N.P.



Characterization of Growth, Water Relations, and Proline Accumulation in Sodium Sulfate Tolerant Callus of Brassica napus L. cv Westar (Canola) 1  

PubMed Central

Unselected and sodium sulfate tolerant callus cultures of Brassica napus L. cv Westar were grown on media supplemented with mannitol, NaCl, or Na2SO4. In all cases, growth of tolerant callus, measured on a fresh weight or dry weight basis, was greater than that of unselected callus, which was also subject to necrosis on high levels of salt. Tissue water potential became more negative in both unselected and tolerant callus grown in the presence of mannitol or Na2SO4. Water potentials in unselected callus were more negative than those of the tolerant tissues; but over a range of Na2SO4 concentrations both cultures displayed osmotic adjustment, maintaining relatively constant turgor. Proline accumulation in both unselected and tolerant callus was low (15 to 20 micromoles per gram dry weight) in the absence of stress, but increased on media supplemented with mannitol, NaCl, or Na2SO4. Increases in proline concentration were approximately linear in tolerant callus, reaching a maximum of 130 to 175 micromoles per gram dry weight. In unselected callus, concentrations were higher, reaching 390 to 520 micromoles per gram dry weight. Proline accumulation was correlated with inhibition of growth, and there was a negative correlation between proline concentration and culture age for tolerant callus. PMID:16665381

Chandler, Stephen F.; Thorpe, Trevor A.



Melatonin levels, determined by LC-ESI-MS/MS, fluctuate during the day/night cycle in Vitis vinifera cv Malbec: evidence of its antioxidant role in fruits.  


The identification of melatonin in plants has inspired new investigations to understand its biological function and which endogenous and external factors control its levels in these organisms. Owing to the therapeutical and nutraceutical properties of melatonin, it should be important to develop reliable analytical methods for its quantification in vegetal matrices containing this indoleamine, such as grape and wine. The main objectives of the present study were to test whether melatonin levels fluctuate during the day in berry skins of Vitis vinifera L. cv Malbec, thereby possibly relating its abundance to its putative antioxidant function, to determine whether daylight reaching clusters negatively controls melatonin levels, and to evaluate whether total polyphenols and anthocyanins also change through a 24-hr period. Grapes were harvested throughout the day/night to determine the moment when high levels of these components are present in grapes. The presence of melatonin in grapes was evaluated by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry. It is shown for the first time that melatonin levels fluctuate during the day/night cycle in plants grown under field conditions in a fruit organ of the species Vitis vinifera. We also determined that the diurnal decay of melatonin in berry skins is induced by sunlight, because covered bunches retained higher melatonin levels than exposed ones, thus explaining at least part of the basis of its daily fluctuation. Evidence of melatonin's antioxidant role in grapes is also suggested by monitoring malondialdehyde levels during the day. PMID:21605162

Boccalandro, Hernán E; González, Carina V; Wunderlin, Daniel A; Silva, María F



Volatile organic compounds characterized from grapevine (Vitis vinifera L. cv. Malbec) berries increase at pre-harvest and in response to UV-B radiation.  


Ultraviolet-B solar radiation (UV-B) is an environmental signal with biological effects in plant tissues. Recent investigations have assigned a protective role of volatile organic compounds (VOCs) in plant tissues submitted to biotic and abiotic stresses. This study investigated VOCs in berries at three developmental stages (veraison, pre-harvest and harvest) of Vitis vinifera L. cv. Malbec exposed (or not) to UV-B both, in in vitro and field experiments. By Head Space-Solid Phase Micro Extraction-Gas Chromatography-Electron Impact Mass Spectrometry (HS-SPME-GC-EIMS) analysis, 10 VOCs were identified at all developmental stages: four monoterpenes, three aldehydes, two alcohols and one ketone. Monoterpenes increased at pre-harvest and in response to UV-B in both, in vitro and field conditions. UV-B also augmented levels of some aldehydes, alcohols and ketones. These results along with others from the literature suggest that UV-B induce grape berries to produce VOCs (mainly monoterpenes) that protect the tissues from UV-B itself and other abiotic and biotic stresses, and could affect the wine flavor. Higher emission of monoterpenes was observed in the field experiments as compared in vitro, suggesting the UV-B/PAR ratio is not a signal in itself. PMID:24075072

Gil, Mariana; Bottini, Rubén; Berli, Federico; Pontin, Mariela; Silva, María Fernanda; Piccoli, Patricia



Relationships between harvest time and wine composition in Vitis vinifera L. cv. Cabernet Sauvignon 2. Wine sensory properties and consumer preference.  


A series of five Vitis vinifera L. cv Cabernet Sauvignon wines were produced from sequentially-harvested grape parcels, with alcohol concentrations between 12% v/v and 15.5% v/v. A multidisciplinary approach, combining sensory analysis, consumer testing and detailed chemical analysis was used to better define the relationship between grape maturity, wine composition and sensory quality. The sensory attribute ratings for dark fruit, hotness and viscosity increased in wines produced from riper grapes, while the ratings for the attributes red fruit and fresh green decreased. Consumer testing of the wines revealed that the lowest-alcohol wines (12% v/v) were the least preferred and wines with ethanol concentration between 13% v/v and 15.5% v/v were equally liked by consumers. Partial least squares regression identified that many sensory attributes were strongly associated with the compositional data, providing evidence of wine chemical components which are important to wine sensory properties and consumer preferences, and which change as the grapes used for winemaking ripen. PMID:24518320

Bindon, Keren; Holt, Helen; Williamson, Patricia O; Varela, Cristian; Herderich, Markus; Francis, I Leigh



Effect of boiling time on chemical composition and physico-functional properties of flours from taro (Colocasia esculenta cv fouê) corm grown in Côte d'Ivoire.  


Taro (Colocasia esculenta cv fouê) corm was subjected to different boiling times and the changes in chemical composition and physico-functional properties were investigated using standard method. The change in boiling time led to a significant (P?

Amon, Anon Simplice; Soro, René Yadé; Assemand, Emma Fernande; Dué, Edmond Ahipo; Kouamé, Lucien Patrice



Breeding for blast-disease-resistant and high-yield Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutants using low-energy ion beams  

NASA Astrophysics Data System (ADS)

Low-energy ion beam was applied on mutation induction for plant breeding of blast-disease-resistant Thai jasmine rice (Oryza sativa L. cv. KDML 105). Seeds of the wild-type rice were bombarded in vacuum by nitrogen ion beam at energy of 60-80 keV to a beam fluence range of 2 × 1016-2 × 1017 ions/cm2. The ion-bombarded rice seeds were grown in soil for 2 weeks as transplanted rice in plastic pots at 1 seedling/pot. The seedlings were then screened for blast resistance by Pyricularia grisea inoculation with 106 spores/ml concentrations. The blast-resistant rice mutant was planted up to F6 generation with the consistent phenotypic variation. The high percentage of the blast-disease-resistant rice was analyzed with DNA fingerprint. The HAT-RAPD (high annealing temperature-random amplified polymorphic DNA) marker revealed the modified polymorphism fragment presenting in the mutant compared with wild type (KDML 105). The cDNA fingerprints were investigated and the polymorphism fragment was subcloned into pGEM-T easy vector and then sequenced. The sequence of this fragment was compared with those already contained in the database, and the fragment was found to be related to the Spotted leaf protein 11 (Spl11).

Mahadtanapuk, S.; Teraarusiri, W.; Phanchaisri, B.; Yu, L. D.; Anuntalabhochai, S.



The effects of Co2+ and Zn2+ on the contents of protein, abscisic acid, proline and chlorophyll in bean (Phaseolus vulgaris cv. Strike) seedlings.  


17-day-old bean plants (Phaseolus vulgaris cv. Strike) were used to analyze the effects of Co2+ and Zn2+ on the time course of proline, total protein, chlorophyll and abscisic acid (ABA) levels in leaves. Controls, Co2+ and Zn2+-treated plants were grown for 8 days in Hoagland solution. Samples were taken at 2 day intervals. Proline, chlorophyll (a+b) and total protein contents of 17 day old primary leaves were determined by a spectrophotometer. ABA contents in roots and leaves of the seedlings were measured by high-pressure liquid chromatography. The presence of Zn2+ and Co2+ significantly increased the ABA contents in roots and leaves (p < 0.05 or p < 0.01). The increase of the abscisic acid content in the leaves was related to the content of the roots. This was further substantiated by enhanced accumulation of proline in the leaves of seedlings exposed to zinc and cobalt. The contents of chlorophyll (a+b) and total protein decreased with the concentration of both metals (p < 0.05 or p < 0.01). Cobalt proved to be comparatively more toxic than zinc. PMID:17436541

Zengin, Fikriye Kirbag



Simultaneous treatment to attain blood pressure and lipid goals and reduced CV risk burden using amlodipine/atorvastatin single-pill therapy in treated hypertensive participants in a randomized controlled trial.  


TOGETHER investigated whether targeting multiple cardiovascular (CV) risk factors using single-pill amlodipine/atorvastatin (AML/ATO) and therapeutic lifestyle changes (TLC) results in greater blood pressure (BP)/lipid control and additional reduction in estimated cardiovascular disease (CVD) risk compared with blood pressure intervention only using amlodipine (AML) + TLC. TOGETHER was a 6-week, randomized, double-blind, double-dummy trial using hypertensive participants with additional CV risk factors without CVD/diabetes. Participants were randomized to either AML/ATO (5 to 10/20 mg) + TLC or AML (5 to 10 mg) + TLC. The primary end point was the difference in proportion of participants attaining both BP (<140/90 mm Hg) and low-density lipoprotein cholesterol (LDL-C) (<100 mg/dL) goals at week 6. At week 6, 67.8% of participants receiving AML/ATO + TLC attained the combined BP/LDL-C goal versus 9.6% with AML + TLC (RD [A-B]: 58.2; 95% CI [48.1 to 68.4] P < 0.001; OR: 19.0; 95% CI 9.1 to 39.6; P < 0.001). Significant reductions from baseline in LDL-C, total cholesterol and triglycerides and estimated 10-year Framingham risk were also observed. Treatment with AML/ATO was well tolerated. In conclusion, a multifactorial CV management approach is more effective in achieving combined BP/LDL-C targets as well as CV risk reduction compared with BP intervention only in this patient population. PMID:20479948

Grimm, Richard; Malik, Mobin; Yunis, Carla; Sutradhar, Santosh; Kursun, Attila



Blockade of TNF in vivo using cV1q antibody reduces contractile dysfunction of skeletal muscle in response to eccentric exercise in dystrophic mdx and normal mice  

Microsoft Academic Search

This study evaluated the contribution of the pro-inflammatory cytokine, tumour necrosis factor (TNF) to the severity of exercise-induced muscle damage and subsequent myofibre necrosis in mdx mice. Adult mdx and non-dystrophic C57 mice were treated with the mouse-specific TNF antibody cV1q before undergoing a damaging eccentric contraction protocol performed in vivo on a custom built mouse dynamometer. Muscle damage was

A. T. Piers; T. Lavin; H. G. Radley-Crabb; A. J. Bakker; M. D. Grounds; G. J. Pinniger



The study of the electrooxidation of chloride at RuO 2\\/TiO 2 electrode using CV and radiotracer techniques and evaluating by electrochemical kinetic simulation methods  

Microsoft Academic Search

The mechanism of the chloride electrooxidation was investigated by CV and radiotracer techniques, as well as PC simulation methods on RuO2\\/TiO2 mixed oxide electrodes. The voltammetric behavior of an adsorbed intermediate was studied.Both the experimental and simulation results show that the electrooxidation of chloride is going through the steps of the Volmer–Heyrovsky mechanism on this working electrode.

L. Tomcsányi; A. De Battisti; G. Hirschberg; K. Varga; J. Liszi



Evaluation of green fluorescent protein as a reporter gene and phosphinothricin as the selective agent for achieving a higher recovery of transformants in cucumber ( Cucumis sativus L. cv. Poinsett76) via Agrobacterium tumefaciens  

Microsoft Academic Search

Efficient Agrobacterium tumefaciens-mediated transformation and a higher recovery of transformed plants of cucumber cv. Poinsett76 were achieved via direct organogenesis\\u000a from cotyledon explants. Stable transformants were obtained by inoculating explants with A. tumefaciens strains EHA105 or LBA4404, both harboring the binary vector pME508, which contains the neomycin phosphotransferase II (nptII) and phosphinothricin resistance genes (bar) conferring resistance to kanamycin and

N. Selvaraj; S. Kasthurirengan; A. Vasudevan; M. Manickavasagam; C. W. Choi; A. Ganapathi



Isolation, identification and sequence analysis of a thioredoxin h gene, a member of subgroup III of h-type Trxs from grape (Vitis vinifera L. cv. Askari).  


Thioredoxins (Trxs) are small ubiquitous proteins which play a regulatory role in a variety of cellular processes. In contrast to other organisms, plants have a great number of Trx types, consisting of six well-defined groups: f, m, x, and y in chloroplasts, o in mitochondria, and h mainly in cytosol. A full-length cDNA, designated VvCxxS2, encoding Trx h polypeptide was isolated and cloned from grape (Vitis vinifera L. cv. Askari) berries organ by reverse transcription polymerase chain reaction (RT-PCR). The cDNA was 381 bp nucleotides in length with a deduced amino acid of 126 residues, possessing a WCIPS active site, which belongs to the subgroup III of h-type Trxs based on phylogenetic analysis. The calculated molecular mass and the predicted isoelectric point of the deduced polypeptide are 14.25 kDa and 4.68, respectively. Nucleotide sequence analysis of genomic DNA fragment of VvCxxS2 gene revealed that this gene possesses two introns at positions identical to the previously sequenced Trx h genes. A modeling analysis indicated that VvCxxS2 shares a common structure with other Trxs, and is preferably reduced by Grx rather than NADPH-dependent thioredoxin reductase (NTR). The deduced protein sequence showed a high similarity to Trx h from other plants, in particular from castor bean (Ricinus communis), Betula pendula and sweet orange (Citrus sinensis). Semiquantitative RT-PCR experiments indicated that the transcripts of VvCxxS2 gene are present in all plant organs and different developmental stages. In addition, the higher expression of the VvCxxS2 gene was observed in berry organ as compared to the other organs. PMID:21732058

Japelaghi, Reza Heidari; Haddad, Raheem; Garoosi, Ghasem-Ali



Generation of a predicted protein database from EST data and application to iTRAQ analyses in grape (Vitis vinifera cv. Cabernet Sauvignon) berries at ripening initiation  

PubMed Central

Background iTRAQ is a proteomics technique that uses isobaric tags for relative and absolute quantitation of tryptic peptides. In proteomics experiments, the detection and high confidence annotation of proteins and the significance of corresponding expression differences can depend on the quality and the species specificity of the tryptic peptide map database used for analysis of the data. For species for which finished genome sequence data are not available, identification of proteins relies on similarity to proteins from other species using comprehensive peptide map databases such as the MSDB. Results We were interested in characterizing ripening initiation ('veraison') in grape berries at the protein level in order to better define the molecular control of this important process for grape growers and wine makers. We developed a bioinformatic pipeline for processing EST data in order to produce a predicted tryptic peptide database specifically targeted to the wine grape cultivar, Vitis vinifera cv. Cabernet Sauvignon, and lacking truncated N- and C-terminal fragments. By searching iTRAQ MS/MS data generated from berry exocarp and mesocarp samples at ripening initiation, we determined that implementation of the custom database afforded a large improvement in high confidence peptide annotation in comparison to the MSDB. We used iTRAQ MS/MS in conjunction with custom peptide db searches to quantitatively characterize several important pathway components for berry ripening previously described at the transcriptional level and confirmed expression patterns for these at the protein level. Conclusion We determined that a predicted peptide database for MS/MS applications can be derived from EST data using advanced clustering and trimming approaches and successfully implemented for quantitative proteome profiling. Quantitative shotgun proteome profiling holds great promise for characterizing biological processes such as fruit ripening initiation and may be further improved by employing preparative techniques and/or analytical equipment that increase peptide detection sensitivity via a shotgun approach. PMID:19171055

Lucker, Joost; Laszczak, Mario; Smith, Derek; Lund, Steven T



Changes in pectin methyl esterase activity with different packaging materials and stages of fruit harvesting during cold storage of pear cv. Punjab beauty.  


Pear cv. Punjab Beauty has become quite popular in Punjab. Excessive softening during cold storage leading to low shelf life is the major factor limiting its wider adoption. Studies were, therefore, conducted to determine the firmness and pectin methyl esterase (PME) activity at 4 harvest dates (2nd, 3rd and 4th week of July, and 1st week of August). Various packaging materials i.e. corrugated fiber board boxes and crates with high and low density polyethylene liners, corrugated fiber board boxes, crates and wooden boxes were also evaluated for their role in extending the shelf life of fruits. The enzyme activity and fruit firmness was evaluated periodically after 30, 45, 60 and 75 days of storage at 0-1 °C and 90-95 % RH. The firmness of the fruits decreased with the increase in storage intervals but the enzyme activity increased with the storage period up to 60 days and declined thereafter. Ripening-related changes in all the harvests were characterized mainly by an increase in the solubilization of pectin with a concomitant decrease in the degree of firmness. There was a continuous increase in enzyme activity with the advancement in harvesting dates and then fell sharply in the advanced ripening stages. Highest pectin methyl esterase activity was in fruits packed in crates followed by wooden boxes and corrugated fiber board boxes while the lowest was recorded in fruits packed in corrugated fiber board boxes with high density polyethylene liners. Therefore, high density polyethylene lined CFB boxes proved to be most effective in preventing the loss in firmness. PMID:25328240

Kaur, Kirandeep; Dhillon, W S; Mahajan, B V C



A Novel Late Embryogenesis Abundant Like Protein Associated with Chilling Stress in Nicotiana tabacum cv. Bright Yellow-2 Cell Suspension Culture*  

PubMed Central

Low temperature is one of the major abiotic stresses limiting the productivity and geographical distribution of many important crops. To identify proteins associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 (BY-2) cell suspension culture, we utilized a proteomic approach with two-dimensional electrophoresis to compare proteins from samples of treated with or without chilling treatment at 4 °C. One protein specifically more abundant in chilling treated sample was identified and designated as NtLEA7-3. Rapid amplification of cDNA ends gave rise to a full-length NtLEA7-3 cDNA with a complete open reading frame of 1267 bp, encoding a 322 amino acid polypeptide. Homology search and sequence multi-alignment demonstrated that the deduced NtLEA7-3 protein sequence shared a high identity with LEA-like proteins from other plants. Subcellular localization analysis indicated that the NtLEA7-3 was localized exclusively in the nucleus. When the gene was overexpressed in bright yellow-2 cells, the transgenic bright yellow-2 cells show more resistant to chilling stress than the wild-type cells. In addition, transgenic Arabidopsis plants overexpressing the NtLEA7-3 are much more resistant to cold, drought, and salt stresses. Interestingly, the expression of NtLEA7-3 in tobacco was not tissue-specific and induced by chilling, drought and salt stresses. All of these, taken together, suggest that NtLEA7-3 is worthwhile to elucidate the contribution of the proteins to the tolerance mechanism to chilling stress, and can be considered as a potential target for crop genetic improvement in the future. PMID:21653253

Gai, Ying-Ping; Ji, Xian-Ling; Lu, Wei; Han, Xue-Juan; Yang, Guo-Dong; Zheng, Cheng-Chao



Salicylic Acid Alleviates the Adverse Effects of Salt Stress in Torreya grandis cv. Merrillii Seedlings by Activating Photosynthesis and Enhancing Antioxidant Systems  

PubMed Central

Background Salt stress is a major factor limiting plant growth and productivity. Salicylic acid (SA) has been shown to ameliorate the adverse effects of environmental stress on plants. To investigate the protective role of SA in ameliorating salt stress on Torreya grandis (T. grandis) trees, a pot experiment was conducted to analyze the biomass, relative water content (RWC), chlorophyll content, net photosynthesis (Pn), gas exchange parameters, relative leakage conductivity (REC), malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD) and peroxidase (POD) of T. grandis under 0.2% and 0.4% NaCl conditions with and without SA. Methodology/Principal Findings The exposure of T. grandis seedlings to salt conditions resulted in reduced growth rates, which were associated with decreases in RWC and Pn and increases in REC and MDA content. The foliar application of SA effectively increased the chlorophyll (chl (a+b)) content, RWC, net CO2 assimilation rates (Pn), and proline content, enhanced the activities of SOD, CAT and POD, and minimized the increases in the REC and MDA content. These changes increased the capacity of T. grandis in acclimating to salt stress and thus increased the shoot and root dry matter. However, when the plants were under 0% and 0.2% NaCl stress, the dry mass of the shoots and roots did not differ significantly between SA-treated plants and control plants. Conclusions SA induced the salt tolerance and increased the biomass of T. grandis cv. by enhancing the chlorophyll content and activity of antioxidative enzymes, activating the photosynthetic process, and alleviating membrane injury. A better understanding about the effect of salt stress in T. grandis is vital, in order gain knowledge over expanding the plantations to various regions and also for the recovery of T. grandis species in the future. PMID:25302987

Du, Xuhua; Tang, Hui; Shen, Chaohua; Wu, Jiasheng



Changes after Decapitation in Concentrations of Indole-3-Acetic Acid and Abscisic Acid in the Larger Axillary Bud of Phaseolus vulgaris L. cv Tender Green 1  

PubMed Central

Early changes in the concentrations of indole-3-acetic acid (IAA) and abscisic acid (ABA) were investigated in the larger axillary bud of 2-week-old Phaseolus vulgaris L. cv Tender Green seedlings after removal of the dominant apical bud. Concentrations of these two hormones were measured at 4, 6, 8, 12 and 24 hours following decapitation of the apical bud and its subtending shoot. Quantitations were accomplished using either gas chromatography-mass spectrometry-selected ion monitoring (GS-MS-SIM) with [13C6]-IAA or [2H6]-ABA as quantitative internal standards, or by an indirect enzyme-linked immunosorbent assay, validated by GC-MS-SIM. Within 4 hours after decapitation the IAA concentration in the axillary bud had increased fivefold, remaining relatively constant thereafter. The concentration of ABA in axillary buds of decapitated plants was 30 to 70% lower than for buds of intact plants from 4 to 24 hours following decapitation. Fresh weight of buds on decapitated plants had increased by 8 hours after decapitation and this increase was even more prominent by 24 hours. Anatomical assessment of the larger axillary buds at 0, 8, and 24 hours following decapitation showed that most of the growth was due to cell expansion, especially in the intermodal region. Thus, IAA concentration in the axillary bud increases appreciably within a very few hours of decapitation. Coincidental with the rise in IAA concentration is a modest, but significant reduction in ABA concentration in these axillary buds after decapitation. Images Figure 4 Figure 6 PMID:16667989

Gocal, Greg F. W.; Pharis, Richard P.; Yeung, Edward C.; Pearce, David



Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells.  


Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. One hypothesis is that the extracellular compounds produced by the fungi associated with grapevine trunk diseases are responsible for pathogenicity.In the present work, we used Vitis vinifera cv. Chardonnay cells to test the aggressiveness of total extracellular compounds produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with botryosphaeria dieback. Additionally, the toxicity of purified mellein, a characteristic toxin present in the extracellular compounds of Botryosphaeriaceae, was assessed.Our results show that the total extracellular compounds produced by N. parvum induce more necrosis on Chardonnay calli and induce a different defence gene expression pattern than those of D. seriata. Mellein was produced by both fungi in amounts proportional to its aggressiveness. However, when purified mellein was added to the culture medium of calli, only a delayed necrosis and a lower-level expression of defence genes were observed. Extracellular compounds seem to be involved in the pathogenicity of the fungi associated with botryosphaeria dieback. However, the doses of mellein used in this study are 100 times higher than those found in the liquid fungal cultures: therefore, the possible function of this toxin is discussed. PMID:24752796

Ramírez-Suero, M; Bénard-Gellon, M; Chong, J; Laloue, H; Stempien, E; Abou-Mansour, E; Fontaine, F; Larignon, P; Mazet-Kieffer, F; Farine, S; Bertsch, C



Significant changes in cell and chloroplast development in young wheat leaves (Triticum aestivum cv Hereward) grown in elevated CO{sub 2}  

SciTech Connect

Cell and chloroplast development were characterized in young Triticum aestivum cv Hereward leaves grown at ambient (350 {mu}L L{sup {minus}1}) or at elevated (650 {mu}L L{sup {minus}1}) CO{sub 2}. In elevated CO{sub 2}, cell and chloroplast expansion was accelerated by 10 and 25%, respectively, in the first leaf of 7-d-old wheat plants without disruption to the leaf developmental pattern. Elevated CO{sub 2} did not affect the number of chloroplasts in relation to mesophyll cell size or the linear relationship between chloroplast number or size and mesophyll cell size. No major changes in leaf anatomy or in chloroplast ultrastructure were detected as a result of growth in elevated CO{sub 2}, but there was a marked reduction in starch accumulation. In leaf sections fluorescently tagged antisera were used to visualize and quantitate the amount of cytochrome f, the {alpha}- and {beta}-subunits of the coupling factor 1 in ATP synthase, D1 protein of the photosystem II reaction center, the 33-kD protein of the extrinsic oxygen-evolving complex, subunit II of photosystem I, and ribulose-1,5-biphosphate carboxylase/oxygenase. A significant finding was that in 10 to 20% of the mesophyll cells grown in elevated CO{sub 2} the 33-kD protein of the extrinsic oxygen-evolving complex of photosystem II and cytochrome f were deficient by 75%, but the other proteins accumulated normally. 29 refs., 6 figs., 2 tabs.

Robertson, E.J.; Leech, R.M. [Univ. of York, Heslington (United Kingdom)



Effects of bermuda grass (Cynodon dactylon cv. Coast cross) and rice hulls on growth performance of 30 day-old weaned rabbits.  


Several studies have demonstrated that rabbits can be maintained on diets containing high levels of Crude Fiber (CF) when compared to other monogastric animals. In the present study, we examined the effects of rice hulls and of bermuda grass (cv. Coast cross) on the growing performance of 30 day-old weaned rabbits. Rabbits were fed one of 5 diets containing rice hulls and/or bermuda grass as fiber source at the following proportions (BG/RH) 0/17.5, 15/11, 0/29, 14.7/19.1 and 48.5/0 for diets A, B, C, D and E, respectively. Body weight gain and voluntary feed intake were measured at 30, 44, 58 and 72 days. The time necessary to attain 2 kg of live body weight was not affected by the diets. However, daily weight gain differed significantly during the first two weeks after weaning among dietary groups. Diets C and D caused a lower body weight gain, probably because of the high level of Acid Detergent Fiber (ADF) in the diet (24% and 23%). Voluntary feed intake increased with age in all treatments, but food intake was lower in treatments C and D when compared to animals receiving treatments B and E. The present results demonstrate that when rice hulls are used as fiber source, fiber must be given as ADF and not as CF because the difference between ADF and CF is enormous. Rice hull-containing diets balanced with CF give an inappropriate amount of components that seem to affect the growth performance of young animals. PMID:7872830

Gierus, M; Rocha, J B; Warpechowski, M B; Riegel, R E



Effect of pulsed electric field treatment on enzyme kinetics and thermostability of endogenous ascorbic acid oxidase in carrots (Daucus carota cv. Nantes).  


The objective of this research was to study the enzyme kinetics and thermostability of endogenous ascorbic acid oxidase (AAO) in carrot purée (Daucus carota cv. Nantes) after being treated with pulsed electric field (PEF) processing. Various PEF treatments using electric field strength between 0.2 and 1.2kV/cm and pulsed electrical energy between 1 and 520kJ/kg were conducted. The enzyme kinetics and the kinetics of AAO thermal inactivation (55-70°C) were described using Michaelis-Menten model and first order reaction model, respectively. Overall, the estimated Vmax and KM values were situated in the same order of magnitude as the untreated carrot purée after being exposed to pulsed electrical energy between 1 and 400kJ/kg, but slightly changed at pulsed electrical energy above 500kJ/kg. However, AAO presented different thermostability depending on the electric field strength applied. After PEF treatment at the electric field strength between 0.2 and 0.5kV/cm, AAO became thermolabile (i.e. increase in inactivation rate (k value) at reference temperature) but the temperature dependence of k value (Ea value) for AAO inactivation in carrot purée decreased, indicating that the changes in k values were less temperature dependent. It is obvious that PEF treatment affects the temperature stability of endogenous AAO. The changes in enzyme kinetics and thermostability of AAO in carrot purée could be related to the resulting carrot purée composition, alteration in intracellular environment and the effective concentration of AAO released after being subjected to PEF treatment. PMID:24176379

Leong, Sze Ying; Oey, Indrawati



Relationship between Freezing Tolerance of Root-Tip Cells and Cold Stability of Microtubules in Rye (Secale cereale L. cv Puma) 1  

PubMed Central

The response of cortical microtubules to low temperature and freezing was assessed for root tips of cold-acclimated and non-acclimated winter rye (Secale cereale L. cv Puma) seedlings using indirect immunofluorescence microscopy with antitubulin antibodies. Roots cooled to 0 or ?3°C were fixed for immunofluorescence microscopy at these temperatures or after an additional hour at 4°C. Typical arrays of cortical microtubules were present in root-tip cells of seedlings exposed to the cold-acclimation treatment of 4°C for 2 days. Microtubules in these cold-acclimated cells were more easily depolymerized by a 0°C treatment than microtubules in root-tip cells of nonacclimated, 22°C-grown seedlings. Microtubules were still present in some cells of both nonacclimated and cold-acclimated roots at 0 and ?3°C; however, the number of microtubules in these cells was lower than in controls. Microtubules remaining during the ?3°C freeze were shorter than microtubules in unfrozen control cells. Repolymerization of microtubules after both the 0 and ?3°C treatments occurred within 1 h. Root tips of nonacclimated seedlings had an LT-50 of ?9°C. Cold acclimation lowered this value to ?14°C. Treatment of 22°C-grown seedlings for 24 h with the microtubule-stabilizing drug taxol caused a decrease in the freezing tolerance of root tips, indicated by a LT-50 of ?3°C. Treatment with D-secotaxol, an analog of taxol that was less effective in stabilizing microtubules, did not alter the freezing tolerance. We interpret these data to indicate that a degree of depolymerization of microtubules is necessary for realization of maximum freezing tolerance in root-tip cells of rye. Images Figure 1 Figure 2 PMID:16667470

Kerr, Gregory P.; Carter, John V.




SciTech Connect

We observed the accreting white dwarf (WD) 1E1339.8+2837 (1E1339) in the globular cluster M3 in 2003 November, 2004 May, and 2005 January, using the Chandra ACIS-S detector. The source was observed in 1992 to possess traits of a supersoft X-ray source (SSS), with a 0.1-2.4 keV luminosity as large as 2 x 10{sup 35} erg s{sup -1}, after which time the source's luminosity fell by roughly two orders of magnitude, adopting a hard X-ray spectrum more typical of cataclysmic variables (CVs). Our observations confirm 1E1339's hard CV-like spectrum, with photon index {Gamma} = 1.3 {+-} 0.2. We found 1E1339 to be highly variable, with a 0.5-10 keV luminosity ranging from (1.4 {+-} 0.3) x 10{sup 34} erg s{sup -1} to 8.5{sup +4.9}{sub -4.6} x 10{sup 32} erg s{sup -1}, with 1E1339's maximum luminosity being perhaps the highest yet recorded for hard X-ray emission from a WD. In 2005 January, 1E1339 displayed substantial low-energy emission below {approx}0.3 keV. Although current Chandra responses cannot properly model this emission, its bolometric luminosity appears comparable to or greater than that of the hard spectral component. This raises the possibility that the supersoft X-ray emission seen from 1E1339 in 1992 may have shifted to the far-UV.

Stacey, W. S.; Heinke, C. O. [Physics Department, University of Alberta, 11322-89 Avenue, Edmonton, AB T6G 2G7 (Canada); Elsner, R. F.; Weisskopf, M. C. [NASA Marshall Space Flight Center, VP62, Huntsville, AL 35812 (United States); Edmonds, P. D.; Grindlay, J. E., E-mail: [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States)



Curdlan ?-1,3-Glucooligosaccharides Induce the Defense Responses against Phytophthora infestans Infection of Potato (Solanum tuberosum L. cv. McCain G1) Leaf Cells  

PubMed Central

Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H2O2 and salicylic acid and the activities of phenylalanine amino-lyase, ?-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually. PMID:24816730

Li, Jing; Zhu, Li; Lu, Guangxing; Zhan, Xiao-Bei; Lin, Chi-Chung; Zheng, Zhi-Yong



A role for PacMYBA in ABA-regulated anthocyanin biosynthesis in red-colored sweet cherry cv. Hong Deng (Prunus avium L.).  


The MYB transcription factors and plant hormone ABA have been suggested to play a role in fruit anthocyanin biosynthesis, but supporting genetic evidence has been lacking in sweet cherry. The present study describes the first functional characterization of an R2R3-MYB transcription factor, PacMYBA, from red-colored sweet cherry cv. Hong Deng (Prunus avium L.). Transient promoter assays demonstrated that PacMYBA physically interacted with several anthocyanin-related basic helix-loop-helix (bHLH) transcription factors to activate the promoters of PacDFR, PacANS and PacUFGT, which are thought to be involved in anthocyanin biosynthesis. Furthermore, the immature seeds of transgenic Arabidopsis plants overexpressing PacMYBA exhibited ectopic pigmentation. Silencing of PacMYBA, using a Tobacco rattle virus (TRV)-induced gene silencing technique, resulted in sweet cherry fruit that lacked red pigment. ABA treatment significantly induced anthocyanin accumulation, while treatment with the ABA biosynthesis inhibitor nordihydroguaiaretic acid (NDGA) blocked anthocyanin production. PacMYBA expression peaked after 2 h of pre-incubation in ABA and was 15.2-fold higher than that of sweet cherries treated with NDGA. The colorless phenotype was also observed in the fruits silenced in PacNCED1, which encodes a key enzyme in the ABA biosynthesis pathway. The endogenous ABA content as well as the transcript levels of six structural genes and PacMYBA in PacNCED1-RNAi (RNA interference) fruit were significantly lower than in the TRV vector control fruit. These results suggest that PacMYBA plays an important role in ABA-regulated anthocyanin biosynthesis and ABA is a signal molecule that promotes red-colored sweet cherry fruit accumulating anthocyanin. PMID:24443499

Shen, Xinjie; Zhao, Kai; Liu, Linlin; Zhang, Kaichun; Yuan, Huazhao; Liao, Xiong; Wang, Qi; Guo, Xinwei; Li, Fang; Li, Tianhong



CV Nils Christophersen October 2007  

E-print Network

-chemical modelling of effects of acid rain on soils and freshwaters. This work has put him on the list of the world became interested in commercialization of academic research and in 1999 he founded the Norwegian School-month working period in start-up companies abroad. In 2006, there were 150 Norwegian students from 19

Sahay, Sundeep


CV and LMXB Population Synthesis  

E-print Network

I discuss population synthesis methods in the context of low-mass compact binaries. Two examples, both constraining the largely unknown strength of orbital angular momentum losses, illustrate the power and problems of such studies. For CVs, the ``standard'' disrupted magnetic braking model predicts that systems below the period gap are on average older than systems above the gap. The corresponding difference in the space velocity dispersion is testable by observations, independent of brightness-dependent selection effects. For LMXBs, the fraction of transients among short-period neutron star systems turns out to be an important diagnostic quantity constraining not only the angular momentum loss rate but also the kick velocity imparted to the neutron star at birth and the common envelope efficiency. Small kicks (less than 100 km/s), low efficiencies and weak magnetic braking are strongly favoured.

Ulrich Kolb



Biradavolu_CV October 2011  

E-print Network

women's life contexts." Social Science and Medicine, 72(5): 710-716. 2011 Biradavolu, Monica, Kim M M. Blankenship. "The context of economic insecurity & relation to violence and risk factors for HIV, Punam Keller and Barbara Rimer."Informing women about their breast cancer risk: Truth and consequences

Lansky, Joshua


CV Sergienko 1 Curriculum Vitae  

E-print Network

. Dissertation: Surface melting on ice shelves and icebergs. Advisor: Douglas R. MacAyeal 1999 Candidate sponsored, David E. Archer, P.I.), Collaborative Research on Earth's Largest Icebergs (NSF sponsored experience Austral summers, three seasons, 2004-2006: Icebergs of the Ross Sea, the Ross Ice Shelf and Mc

Rodgers, Keith


CV Sergienko 1 Curriculum Vitae  

E-print Network

. Dissertation: Surface melting on ice shelves and icebergs. Advisor: Douglas R. MacAyeal 1999 Candidate in the Equatorial Region (NSF sponsored, David E. Archer, P.I.), Collaborative Research on Earth's Largest Icebergs as above. Field experience Austral summers, three seasons, 2004-2006: Icebergs of the Ross Sea, the Ross

Rodgers, Keith


ROGER WIEGAND'S CV October 2011  

E-print Network

in Commutative Algebra and Algebraic Geometry (group infrastructure support­no salary). · NSA Grant 01G-144, $41 Conference (May 2005), $12,000. (A. Li and L. Avramov were the PIs, but I wrote much of the proposal.) · NSA

Logan, David


Nadia L. Zakamska CV Information  

E-print Network

, funded) Herschel (Sept 2011, funded) Gemini (GMOS, March 2010) Gemini (NIFS, March 2010) APO 3.5m (Triplespec, spring 2009, summer 2009) Gemini (GMOS, March 2006) Chandra (March 2005, funded) Spitzer (Nov proposals: Gemini (GMOS, Sept 2012) Spitzer warm mission Spies survey (legacy proposal, funded, Aug 2012

Weaver, Harold A. "Hal"



E-print Network

Association for Symbolic Logic per la tesi di dottorato: Applications of the proper forcing axiom to cardinal of Symbolic Logic 14(1), 99-113, 2008. · The Proper Forcing Axiom and the Singular Cardinal Hypothesis(4), 3137-3139, 2005. · On the mapping reflection principle MRP, The Bulletin of Symbolic Logic 15(3), 322

Viale, Matteo


CV Sander Nieuwenhuis Personal data  

E-print Network

.-J. (submitted). Two birds with one stone: A preregistered adversarial collaboration on horizontal eye movements., & Nieuwenhuis, S. (submitted). Moral impression management: Evaluation by an ingroup member during a moral IAT enhances perceptual attention and conflict monitoring. #12;Harsay, H.A., Cohen, M.X., Spaan, M., Weeda, W

Nieuwenhuis, Sander


Transcriptome analysis at four developmental stages of grape berry (Vitis vinifera cv. Shiraz) provides insights into regulated and coordinated gene expression  

PubMed Central

Background Vitis vinifera berry development is characterised by an initial phase where the fruit is small, hard and acidic, followed by a lag phase known as veraison. In the final phase, berries become larger, softer and sweeter and accumulate an array of organoleptic compounds. Since the physiological and biochemical makeup of grape berries at harvest has a profound impact on the characteristics of wine, there is great interest in characterising the molecular and biophysical changes that occur from flowering through veraison and ripening, including the coordination and temporal regulation of metabolic gene pathways. Advances in deep-sequencing technologies, combined with the availability of increasingly accurate V. vinifera genomic and transcriptomic data, have enabled us to carry out RNA-transcript expression analysis on a global scale at key points during berry development. Results A total of 162 million 100-base pair reads were generated from pooled Vitis vinifera (cv. Shiraz) berries sampled at 3-weeks post-anthesis, 10- and 11-weeks post-anthesis (corresponding to early and late veraison) and at 17-weeks post-anthesis (harvest). Mapping reads from each developmental stage (36-45 million) onto the NCBI RefSeq transcriptome of 23,720 V. vinifera mRNAs revealed that at least 75% of these transcripts were detected in each sample. RNA-Seq analysis uncovered 4,185 transcripts that were significantly upregulated at a single developmental stage, including 161 transcription factors. Clustering transcripts according to distinct patterns of transcription revealed coordination in metabolic pathways such as organic acid, stilbene and terpenoid metabolism. From the phenylpropanoid/stilbene biosynthetic pathway at least 46 transcripts were upregulated in ripe berries when compared to veraison and immature berries, and 12 terpene synthases were predominantly detected only in a single sample. Quantitative real-time PCR was used to validate the expression pattern of 12 differentially expressed genes from primary and secondary metabolic pathways. Conclusions In this study we report the global transcriptional profile of Shiraz grapes at key stages of development. We have undertaken a comprehensive analysis of gene families contributing to commercially important berry characteristics and present examples of co-regulation and differential gene expression. The data reported here will provide an invaluable resource for the on-going molecular investigation of wine grapes. PMID:23227855



A DESD-box helicase functions in salinity stress tolerance by improving photosynthesis and antioxidant machinery in rice (Oryza sativa L. cv. PB1).  


The exact mechanism of helicase-mediated salinity tolerance is not yet understood. We have isolated a DESD-box containing cDNA from Pisum sativum (Pea) and named it as PDH45. It is a unique member of DEAD-box helicase family; containing DESD instead of DEAD/H. PDH45 overexpression driven by constitutive cauliflower mosaic virus-35S promoter in rice transgenic [Oryza sativa L. cv. Pusa Basmati 1 (PB1)] plants confers salinity tolerance by improving the photosynthesis and antioxidant machinery. The Na(+) ion concentration and oxidative stress parameters in leaves of the NaCl (0, 100 or 200 mM) treated PDH45 overexpressing T1 transgenic lines were lower as compared to wild type (WT) rice plants under similar conditions. The 200 mM NaCl significantly reduced the leaf area, plant dry mass, net photosynthetic rate (PN), stomatal conductance (gs), intercellular CO2 (Ci), chlorophyll (Chl) content in WT plants as compared to the transgenics. The T1 transgenics exhibited higher glutathione (GSH) and ascorbate (AsA) contents under salinity stress. The activities of antioxidant enzymes viz. superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and glutathione reductase (GR) were significantly higher in transgenics; suggesting the existence of an efficient antioxidant defence system to cope with salinity induced-oxidative damage. Yeast two-hybrid assay indicated that the PDH45 protein interacts with Cu/Zn SOD, adenosine-5'-phosphosulfate-kinase, cysteine proteinase and eIF(4G), thus confirming the involvement of ROS scavenging machinery in the transgenic plants to provide salt tolerance. Furthermore, the T2 transgenics were also able to grow, flower, and set viable seeds under continuous salinity stress of 200 mM NaCl. This study provides insights into the mechanism of PDH45 mediated salinity stress tolerance by controlling the generation of stress induced reactive oxygen species (ROS) and also by protecting the photosynthetic machinery through a strengthened antioxidant system. PMID:23456247

Gill, Sarvajeet Singh; Tajrishi, Marjan; Madan, Meenu; Tuteja, Narendra



Characterization of a novel Toll/interleukin-1 receptor (TIR)-TIR gene differentially expressed in common bean (Phaseolus vulgaris cv. Othello) undergoing a defence response to the geminivirus Bean dwarf mosaic virus.  


SUMMARY Common bean (Phaseolus vulgaris L.) cultivar (cv.) Othello develops a hypersensitive response-associated vascular resistance to infection by Bean dwarf mosaic virus (BDMV), a single-stranded DNA virus (genus Begomovirus, family Geminiviridae). A PCR-based cDNA subtraction approach was used to identify genes involved in this resistance response. Eighteen clones, potentially involved with BDMV resistance, were identified based upon being up-regulated in BDMV-infected tissues and/or having sequence similarity with known resistance-associated genes. Analysis of these clones revealed potential genes involved in pathogen defence, including pathogenesis-related protein genes and resistance gene analogues (RGAs). Further characterization of one RGA, F1-10, revealed that it encodes a predicted protein with a double Toll/interleukin-1 receptor (TIR) motif. Full-length (F1-10) and spliced (F1-10sp) forms of the RGA were strongly up-regulated in BDMV-infected cv. Othello hypocotyl tissues by 4 days post-inoculation, but not in equivalent mock-inoculated tissues. In agroinfiltration experiments, F1-10, but not F1-10sp, mediated resistance to BDMV in the susceptible common bean cv. Topcrop. By contrast, transgenic Nicotiana benthamiana lines expressing F1-10 or F1-10sp were not resistant to BDMV. Interestingly, when these transgenic lines were inoculated with the potyvirus Bean yellow mosaic virus, some F1-10 lines showed a more severe symptom phenotype compared with non-transgenic control plants. Based on these findings, F1-10 was named: Phaseolus vulgaris VIRUS response TIR-TIR GENE 1 (PvVTT1). PMID:20507487

Seo, Young-Su; Jeon, Jong-Seong; Rojas, Maria R; Gilbertson, Robert L



A fasciclin-domain containing gene, ZeFLA11, is expressed exclusively in xylem elements that have reticulate wall thickenings in the stem vascular system of Zinnia elegans cv Envy.  


The vascular cylinder of the mature stem of Zinnia elegans cv Envy contains two anatomically distinct sets of vascular bundles, stem bundles and leaf-trace bundles. We isolated a full-length cDNA of ZeFLA11, a fasciclin-domain-containing gene, from a zinnia cDNA library derived from in vitro cultures of mesophyll cells induced to form tracheary elements. Using RNA in situ hybridization, we show that ZeFLA11 is expressed in the differentiating xylem vessels with reticulate type wall thickenings and adjacent parenchyma cells of zinnia stem bundles, but not in the leaf-trace bundles that deposit spiral thickenings. Our results suggest a function for this cell-surface GPI-anchored glycoprotein in secondary wall deposition during differentiation of metaxylem tissue with reticulate vessels. PMID:16328545

Dahiya, Preeti; Findlay, Kim; Roberts, Keith; McCann, Maureen C



Graduate Council Meeting Agenda 310 Jack K. Williams Administration Building  

E-print Network

Contemporary Civil Wars k. INTA 672 East Asian Security l. INTA 673 Chinese Domestic Politics in Transition m International Politics of the Middle East p. INTA 687 Domestic Intelligence Operations: Legalities, Policies


The effects on photosynthetic CO{sub 2} assimilation to long-term elevation of atmospheric CO{sub 2} concentration: An assessment of the response of Trifolium Repens L. cv. Blanca grown at F.A.C.E.  

SciTech Connect

Understanding how photosynthetic capacity acclimates to elevated CO{sub 2} concentrations is vital in predicting the response of important grassland species such as Trifolium repens. Previous studies of acclimatization have been carried out in artificial experimental conditions, such as acrylic greenhouses or controlled environment chambers. The advent of FACE technology has enabled a large area of crop to be fumigated in the field, providing more realistic growing conditions. Pure stands of Trifolium repens L. cv. Blanca grown at either 355 or 600{mu}mol mol{sup -1} CO{sub 2} were examined, and their photosynthetic response to elevated Ca determined via gas exchange studies. Rates of photosynthesis of young, fully expanded leaves were increased between 21 and 36% when grown and measured at elevated CO{sub 2}. This increase in A corresponded to a decrease in g{sub S} of between 18 and 52%. No acclimation effect was observed in the most frequently cut stands, whilst the response of stands clipped only 4 times per year was more variable. When down regulation of V{sub cmax} did occur, this was not nearly as marked as that which occurred in 3 other temperate species (Chrysanthemum leucanthemum, Ranunculus friesianus, Plantago lanceolata (L.) J. & C. Presl.), at similar growth regimes. No acclimation of stomatal frequency, SI or pore length was found to occur in the enriched clover stands.

Lewis, C.E.



Induction of Lipid and Oleosin Biosynthesis by (+)-Abscisic Acid and Its Metabolites in Microspore-Derived Embryos of Brassica napus Reston (Biological Responses in the Presence of 8[prime]-Hydroxyabscisic Acid).  

PubMed Central

Microspore-derived (MD) embryos of Brassica napus L. cv Reston were used to test the effects of (+)-abscisic acid ([(+)-ABA]) and its metabolites, 8[prime]-hydroxyabscisic acid (8[prime]-OH ABA) and (-)-phaseic acid (PA), on the accumulation of very long-chain monounsaturated fatty acids (VLCMFAs) and induction of genes encoding a 19-kD oleosin protein and a [delta]15 desaturase during embryogenesis. Developing early to mid-cotyledonary MD embryos at 16 to 19 d in culture were treated with 10 [mu]M hormone/metabolite for 4 d. At various times during incubation, embryos and medium were analyzed to determine levels of hormone/metabolite, VLCMFAs, and oleosin or [delta]15 desaturase transcripts. The VLCMFAs, 20:1 and 22:1, primarily in triacylglycerols, increased by 200% after 72 h in the presence of (+)-ABA and 8[prime]-OH ABA relative to the control. In contrast, treatment with PA for 72 h had little effect (20% increase) on the level of VLCMFAs. The first 24 to 72 h of (+)-ABA treatment were critical in the induction of VLCMFA biosynthesis, with 8[prime]-OH ABA lagging slightly behind (+)-ABA in promoting this response. The accumulation of VLCMFAs was positively correlated with an increase in elongase activity. (+)-ABA and its 8[prime]-OH ABA metabolite induced the accumulation of a 19-kD oleosin transcript within 2 to 4 h in culture. In addition, both (+)-ABA and 8[prime]-OH ABA induced the same level of [delta]15 desaturase transcript by 8 h. PA had no effect on the induction of either oleosin or [delta]15 desaturase transcripts. To our knowledge, this is the first report of the biological activity of 8[prime]-OH ABA and of stimulatory effects of (+)-ABA and 8[prime]-OH ABA on lipid and oleosin biosynthesis. PMID:12228493

Zou, J.; Abrams, G. D.; Barton, D. L.; Taylor, D. C.; Pomeroy, M. K.; Abrams, S. R.



-Christopher Ragan CV, page 1-Curriculum Vitae  

E-print Network

in Macroeconomics to Hydroelectricity engineers brought in by SNC-Lavalin from Mainland China. (6 students, 7days. · Economics, Tenth Canadian Edition (with R. Lipsey), Pearson Education Canada, 2000. · Economi

Barthelat, Francois


CV_Brooks2011 Joanna Brooks  

E-print Network

:// Education September 2008 ­ September 2011 PhD Experimental Psychology and Cognitive Neuroscience (EU funded-Naples and University of Trieste Hemispheric asymmetries in attention and spatial working memory across lifespan). January 2005 ­ September 2006 Master of Research in Psychology (EPSRC funded ­ class 2:1) University of St

Edinburgh, University of


Schultz CV page 1 STEPHEN SCHULTZ  

E-print Network

, M Zikry and K Peters, "Full-spectrum interrogation of fiber Bragg grating sensors for dynamic, and S. M. Schultz, "Temporal response of surface-relief fiber Bragg gratings to high temperature CO2: High Efficiency Volume Grating Coupler 1994 Brigham Young University Provo, UT M.S. Electrical

Wirthlin, Michael J.


Robert Crabtree CV Name: Robert H. Crabtree.  

E-print Network

.K. Education: 1966-70 New Coll., Oxford: B.A. (Malcolm Green) 1970-73 Sussex University: D. Phil. (Joseph Chatt (later Charg� de Recherche). 1977-82 Yale University, Assistant Professor 1982-85 Yale University, Associate Professor (tenure 1983) 1985-present Yale University, Professor (now Whitehead Professor) Honors


CV of Q. X. Huang Curriculum Vitae  

E-print Network

", Principle Investigator 2004-2006, Industry project research, "Combustion optimization system based project research, "Combustion diagnostics system based on the flame image processing in 300MW --------------- Education: Ph.D., 09/2000~06/2005, Engineering Thermal Physics, Zhejiang University. B.Sc., 09


Stav Atir, CV  

E-print Network

on a research project on conformity in memory. Performed analyses of fMRI data (using Brain Voyager in carrying out a research project in psychology" (May 2010) Awarded membership to Phi Beta Kappa SocietyPFC) and analyzing imaging data of healthy controls. · Supervising undergraduate imaging project on asymmetric

Kable, Joe


CV November 2012, Page 1 Michael Kaspari  

E-print Network

) "Towards a spatial ecology of brown food webs: soil nutrients and trees as templates." (6K) NSF Ecology (PI NSF DIGG (with Natalie Clay): Sodium availability regulates brown food web structure ($14,903). NSF performance through community dynamics. ($324K, $634K total) NSF EAGER: Does sodium limit the brown food web

Kaspari, Mike



E-print Network

. (2011). Juvenile transfer and deterrence: Re-examining the effectiveness of a `get tough' policy. Crime; 2003-2005 Community Guidance Center, Indiana, PA Teaching Experience/Classes Taught Juvenile Delinquency/Juvenile Justice Criminological Theory Offender Treatment Corrections Diversity

Wu, Shin-Tson



E-print Network

Juvenile Delinquency/Juvenile Justice Criminological Theory Offender Treatment Corrections Diversity/Multiculturalism in Criminal Justice Research Methods (Graduate and Undergraduate) Minorities and Crime (Graduate Martin: A test of the racial gradient thesis. Journal of Crime and Justice. DOI: 10.1080/0735648X.2013

Van Stryland, Eric


Page 1 of 16 David Vine CV  

E-print Network

, Middletown, CT Thesis with High[est] Honors: "Community & Identity in a Washington, DC Summer Basketball of Diego Garcia. Souls: A Critical Journal of Black Culture, Politics, and Society 10(4): 327-343. 2008.S. Military Base at Diego Garcia. International Migration 42(3): 111-143. 2003 Cloning Culture in the City

Carlini, David


Interface CV Crawler Create a customizable  

E-print Network

platform to simplify the work of sociologists working with Web Data (web pages and emails) Specific sociologists' needs: Simple form to build primary queries, advanced form to construct FLOWR ·Collaborative work

Senellart, Pierre



E-print Network

ECTS "Introduction to Marketing" 2nd year Undergrad Level "Retail Marketing Strategy", 5th year, MSc, 2 INTERESTS Retailing and Consumer Behaviour Shopping Marketing and Merchandising Consumer Values Merchandising Education Marketing Channels Retailing Merchandising Consumer Behaviour Principles of Marketing

Escolano, Francisco



E-print Network

: Nano-Catalysis, Mixed-Metal Oxides, In-situ reactions Brookhaven (Synchrotron AP-PES, AP-STM, AP Mixed-Metal Oxides Berlin (Synchrotron PES, XPS, STM, IRRAS, LEED, Thin Films, Nanoparticles) Lab National Meeting Indianapolis, IN (2013) Organizer "The Role of Oxides in Catalysis: Structure, Selectivity

Homes, Christopher C.


CV Name: Jianjun Yang JIANJUN YANG  

E-print Network

when working at SGM beamline in Canadian Light Source. Currently, I am involved into an international. Environmental Science and Pollution Research, 21:2943-2154. 2. Shi, J.Y., Peng, C., Yang, Y.Q., Yang, J

Sparks, Donald L.


Bohn, CV 1 of 4 Kirsten Bohn  

E-print Network

, Del Mar, CA 1995 Wildlife Conservation Internship, Smithsonian Institution, The National Zoo Presentation Award, 35th Annual North American Symposium on Bat Research 2003 Doctoral Dissertation Improvement Conservation and Research Center. Front Royal, VA Publications Martinez, J.G., Bohn, K.M., Morris, J.S., and R

Bohn, Kirsten


Luis Castaeda CV, August 2013 Curriculum Vitae  

E-print Network

and Contemporary (Fall 2012) Art and Environment in American Culture since 1800 (Spring 2013 (http://artenvironmentamerica.tumblr and Revolution in Latin America (Fall 2013) ( Graduate Seminars

Segraves, Kari A.


NASA/ESA CV-990 spacelab simulation  

NASA Technical Reports Server (NTRS)

Simplified techniques were applied to conduct an extensive spacelab simulation using the airborne laboratory. The scientific payload was selected to perform studies in upper atmospheric physics and infrared astronomy. The mission was successful and provided extensive data relevant to spacelab objectives on overall management of a complex international payload; experiment preparation, testing, and integration; training for proxy operation in space; data handling; multiexperimenter use of common experimenter facilities (telescopes); multiexperiment operation by experiment operators; selection criteria for spacelab experiment operators; and schedule requirements to prepare for such a spacelab mission.

Reller, J. O., Jr.



CV: Thomas Flatt September 3, 2013 1  

E-print Network

. Thomas Flatt SNF Professor Department of Ecology and Evolution University of Lausanne UNIL Sorge in Zoology, Specialization in Population Biology). PROFESSIONAL EXPERIENCE AND POSITIONS HELD 2012-date: SNF, University of Basel, Switzerland. HONORS AND AWARDS 2012-date: Swiss National Science Foundation (SNF

Lehmann, Laurent


CV: Thomas Flatt January 16, 2014 1  

E-print Network

.html POSITIONS 2012-date: SNF Professor (Swiss National Science Science Foundation (SNF) Professorship 2011-12: Fellow, Institute for Advanced Study Berlin of Lifespan Modulation by Reproduction and Diet, Grant # 13C154. Duration: 12 months. CHF 60,000. 2012-: SNF

Lehmann, Laurent



E-print Network

) systems Harvard School of Public Health Department of Environmental Health Research Fellow 2001's Environmental Health - Assessing heavy metal mobility and human exposure around an abandoned lead and zinc mine) Chemical Oceanography Program ­ Assessing the Impact of Hurricanes on Mercury Biogeochemistry


New CV candidate in Kepler field  

NASA Astrophysics Data System (ADS)

During the routine observations of cataclysmic variables DDE 20 and DDE 21 with the Bradford Robotic Telescope's Galaxy camera (0.36-m f/5.3 reflector + 1kx1k CCD, see for more details) we have found the new large-amplitude variable star at the following position (J2000.0): R.A. ...

Denisenko, D.; Hedges, D. G.; Tallon, C. J.; Baruch, J. E. F.



Christopher Stallings CV 2012 Christopher D. Stallings  

E-print Network

($100) 2005 WSN Best Student Paper (Mia Tegner Award), honorable mention 2002 � 2003 Thompson Coral Reef approach to sampling reef fishes (Co-PIs: R. McMichael, T. Switzer, FWRI; commercial industry participants are included) Approved Florida Fish and Wildlife Conservation Commission. $94,835 Functional role of artificial

Meyers, Steven D.


Christopher Stallings CV 2010 Christopher D. Stallings  

E-print Network

Conservation Commission. $94,835 Functional role of artificial reefs in supporting offshore migration of reef), honorable mention 2003 Thompson Coral Reef Graduate Fellowship ($6,950) 2002 Thompson Coral Reef Graduate.S. Forest Service, Gifford Pinchot National Forest, Carson, WA 1998 Field Biologist (Student Conservation

Meyers, Steven D.


Graduate Council Meeting Agenda 310 Jack K. Williams Administration Building  

E-print Network

Science, Technology, Engineering and Mathematics (STEM) Teaching and Learning b. GEOL 640 Geochemistry. INTA 673 Chinese Domestic Politics in Transition (tabled in March 2013) n. INTA 674 US Foreign Policy Policies (tabled in March 2013) g. INTA 645 Women and Nations (tabled in March 2013) h. INTA 646 Foreign


Graduate Council Meeting Minutes 310 Jack K. Williams Administration Building  

E-print Network

were approved with friendly amendments: a. EDCI 620 Science, Technology, Engineering and Mathematics (tabled in March 2013) n. INTA 674 US Foreign Policy in the Persian Gulf (tabled in March 2013) o. INTA in March 2013) f. INTA 634 Politics and Development Policies (tabled in March 2013) g. INTA 645 Women


Yerslt:V of IHaho _9;~cv ~~W~cv~  

E-print Network

;(!/ a;anb tVandal Thingl" TOP: Tile vandal MarchlnQ AIO\\'E: What's band f!ets the students HomecomlnQ without our pumped up for the tame favorite vandal! Joe aQalnst Nonh Texas. vandal pays a visit to the bonfire. #12;IffY: The homecomlnt! football tame a

O'Laughlin, Jay


CV David B. Haviland, KTH CV David B. Haviland 1 of 3  

E-print Network

. 2011 4. Erik Tholén ­ PhD Dec. 2009 5. Jochen Walter ­ Licenciate Nov. 2004, Ph.D. Nov. 2006 6. Silvia. 2005 8. Mattias Urech ­ (w/ Vlad Korenivski) Lic. Feb. 2001, Ph.D. March 2006 9. Jan Johansson ­ ( w. Fabian Gregris, 2006 5. Evelyene Doherty, 2006 6. Frank Weber, 2005 7. Erik Tholen, 2005 8. Jochen Walter

Haviland, David


Curriculum Vitae Alexander K. Converse, Ph.D. 6 Feb 2012 1/22 \\home\\converse\\Converse_grants_CV\\CV\\Converse_CV_.doc  

E-print Network

reviewer, European Journal of Nuclear Medicine and Molecular Imaging 2011- Associate Editorial Board) Education 1980-1984 Reed College, Portland, Oregon, BA, Physics 1985-1993 University of Wisconsin-Madison, PhD, Physics, emphasis experimental nuclear, advisor Willy Haeberli 2000-2002 University


Deborah Lawrence, CV January 2013 1 DEBORAH LAWRENCE  

E-print Network

, VA 22904-4123 (ph) 434-924-0581 (fax) 434-982-2137 EDUCATION Ph. D. May 1998. Reproductive effort and nutrient availability affect resorption in a Costa Rican rainforest. Ecology. 2. *Das R. 2012. Trees, not organic fertilizers, minimize nutrient loss from coffee management systems

Lawrence, Deborah


dvk cv January 2008 p. 1 DENNIS V. KENT  

E-print Network

Oceanographic Institutions for Deep Earth Sampling (JOIDES): COSOD Working Group IV (1981); Ocean History Panel properties of sediments, oceanic basalts, and polar ice. HONORS Petrus Peregrinus Medal, European Geosciences Management International (IODP-MI): Executive Committee, Board of Governors (2003­2007) Joint Oceanographic

Olsen, Paul E.


Highly optimized OpenCV based cell phone  

Microsoft Academic Search

The new advancement in technology made cell phone as a smart phone with highly computing capabilities and low processing power. Currently, achieving real-time image processing routines such as convolution, edge detection, thresholding and some of complex media applications is a challenging task with the smart phone, because of limited memory and power constraints. An open software framework, Symbian OS is

S. Sankaraiah; R. S. Deepthi



March 2013 Badgley CV, p. 1 CATHERINE E. BADGLEY  

E-print Network

of Paleontology and Department of Earth and Environmental Sciences, University of Michigan 1992-2004 Associate Research Scientist, Museum of Paleontology and Department of Geological Sciences, University of Michigan, Museum of Paleontology, University of Michigan 1985-87 Visiting Assistant Professor, Department

Edwards, Paul N.


March 2014 Badgley CV, p. 1 CATHERINE E. BADGLEY  

E-print Network

Scientist, Museum of Paleontology and Department of Earth and Environmental Sciences, University of Michigan 1992-2004 Associate Research Scientist, Museum of Paleontology and Department of Geological Sciences Research Scientist, Museum of Paleontology, University of Michigan 1985-87 Visiting Assistant Professor

Edwards, Paul N.



E-print Network

­ a marketingApplying to graduate school a marketing perspective Understanding graduate study - A Marketing Perspective #12;Keys to Marketing Successy g Show a fit Genuiness Know yourself Know them #12;Know the likelihood of cognitive abnormalities in developing children exposed to biohazardous materials Usedchildren

Puglisi, Joseph


Ana M. Leon CV-2012 1 Revised August 2012  

E-print Network

Child Welfare Pre-Service, In-Service and Re-Certification Training Programs Advertisement Number: CPI RESEARCH REPORTS Latino Access to Child Health & Mental Health Services, Evaluation of Assessment Tool PUBLICATIONS: 4 National Child Welfare Newsletter Articles INVITED JOURNAL GUEST EDITOR: Journal of Social

Wu, Shin-Tson


Isabel Ribeiro | Short CV February 2014 ISABEL RIBEIRO  

E-print Network

and Plug Remote Handling System in Tokamak Building and Hot Cell", F4EGRT27601 (MSRH), funded and Rescue Cask in all predicted missions in Tokamak and Hot Cell buildings, assuming navigation with both wheels of the CTS following and not following the same path, and maximizing the length



Catherine Graham CV (March 2014) Page 1 Catherine Helen Graham  

E-print Network

of Ecology and Evolution Stony Brook University Stony Brook, NY 11789, USA Work e-mail: catherine-doc (Biogeography) PUBLICATIONS Number of time cited ~ 11,000; h-index = 35; i10-index = 54 (80) Graham, C.H., N.H. Graham and K. Böhning-Gaese. 2013. Competition and dispersal ability interact to determine geographic

Davidson, Ana


HARVARDgazette29 April-12 May 2010 VOL. CV NO. 15  

E-print Network

and Communications John Longbrake Senior Director of Communications Kevin Galvin Director of News and Media Relations Editor/Calendar Corydon Ireland Staff Writer Gervis A. Menzies Jr. Editorial Assistant/Sports Alvin million to be used by Harvard College and Harvard Law School. This contribution will establish


HARVARDgazette29 OCT.-11 NOV. 2009 VOL. CV NO. 5  

E-print Network

Director of Communications Kevin Galvin Director of News and Media Relations Terry L. Murphy Managing Ireland Staff Writer Gervis A. Menzies Jr. Editorial Assistant/Sports Alvin Powell Staff Writer Sarah/ THE COLLEGE TRANSITION Parents (left) know that as first-year students their children will have to deal


HARVARDgazette01-14 APRIL 2010 VOL. CV NO. 13  

E-print Network

Director of Communications Kevin Galvin Director of News and Media Relations Terry L. Murphy Managing Ireland Staff Writer Gervis A. Menzies Jr. Editorial Assistant/Sports Alvin Powell Staff Writer HOUSE MASTERS APPOINTED Harvard College Dean Evelynn M. Hammonds announced the appoint- ment of three


HARVARDgazette18-31 MARCH 2010 VOL. CV NO. 12  

E-print Network

of Communications Kevin Galvin Director of News and Media Relations Terry L. Murphy Managing Editor, Harvard Gazette A. Menzies Jr. Editorial Assistant/Sports Alvin Powell Staff Writer Sarah Sweeney CLASSIC COLLEGE VS. ONLINE LEARNING Two top players in the field of higher ed- ucation explored two almost


HARVARDgazette12 NOV.-02 DEC. 2009 VOL. CV NO. 6  

E-print Network

their claim as a core part of College life. #12;Online Highlights Christine Heenan Vice President of Harvard of News and Media Relations Terry L. Murphy Managing Editor, Harvard Gazette Jim Concannon News Editor Assistant/Sports Alvin Powell Staff Writer Sarah Sweeney Production Assistant/Books Colleen Walsh Staff


HARVARDgazette13-26 May 2010 VOL. CV NO. 16  

E-print Network

Director of News and Media Relations Terry L. Murphy Managing Editor, Harvard Gazette Jim Concannon News. Editorial Assistant/Sports Alvin Powell Staff Writer Sarah Sweeney Production Assistant/Books Colleen Walsh she arrived at Radcliffe College in 1967 she was a confused and naïve first- year student. More


Multiplexed CV quantum teleportation for high rates in quantum communication  

E-print Network

A major challenge of today's quantum communication systems lies in the transmission of quantum information with high rates over long distances in the presence of unavoidable losses. Thereby the achievable quantum communication rate is fundamentally limited by the amount of energy that can be transmitted per use of the channel. It is hence vital to develop quantum communication protocols which encode quantum information as energy efficiently as possible. To this aim we investigate continuous-variable quantum teleportation as a method of distributing quantum information. We explore the possibility to encode information on multiple optical modes and derive upper and lower bounds on the achievable quantum channel capacities. This analysis enables us to benchmark single-mode vs. multi-mode entanglement resources. Our research reveals that multiplexing does not only feature an enhanced energy efficiency, significantly increasing the achievable quantum communication rates in comparison to single-mode coding, but als...

Christ, Andreas; Silberhorn, Christine



Burlando CV--April 2014 1 Alfredo Burlando  

E-print Network EDUCATION Ph.D., Economics, Boston University, Boston, MA, May 2010 to The Journal of Development Economics. "Does Malaria Reduce Education? Finding from the Ethiopian Highlands Groups in Uganda" (with Andrea Canidio) "How does Mobile Money Affect Adopter's Social Networks?" (with

Oregon, University of


CV of Reiner Hartenstein  

E-print Network

on reconfigurable computing and related compilation techniques. Supported by various funding agencies and on VLSI design methods and tools. Since about ten years Prof. Hartenstein and his group also works operation principles: counterpart of control-procedural "von Neumann"paradigm), * the reconfigurable Kress

Hartenstein, Reiner


Noise powered amplification in CV quantum key distribution  

E-print Network

Amplification plays a key role in classical communication protocols, where it compensates the unavoidable loss of the signal. However, when we enter the quantum domain this approach starts being problematic as the standard kinds of amplifiers are usually accompanied by excess noise which detrimentally affects the quantum features of used states. Recently, several kinds of "noiseless" amplifiers that do not suffer from this feature have been proposed. Among these amplifiers, one that stands out is the noise powered amplifier, which acts incoherently, amplifying the signal by adding the "right" kind of noise. Here we show that despite the incoherence, which makes the amplifier unsuitable for protocols such as entanglement distillation, the added noise is not a big problem in quantum key distribution tasks and can be in some situations beneficial.

Petr Marek; Radim Filip



Very Bright CV discovered by MASTER-ICATE (Argentina)  

NASA Astrophysics Data System (ADS)

MASTER-ICATE very wide-field camera (d=72mm f/1.2 lens + 11 Mpix CCD) located near San Juan, Argentina has discovered OT source at (RA, Dec) = 14h 20m 23.5s -48d 55m 40s on the combined image (exposure 275 sec) taken on 2013-06-08.048 UT. The OT unfiltered magnitude is 12.1m (limit 13.1m). There is no minor planet at this place. The OT is seen in more than 10 images starting from 2013-06-02.967 UT (275 sec exposure) when it was first detected at 12.4m.

Saffe, C.; Levato, H.; Mallamaci, C.; Lopez, C.; Lipunov, F. Podest V.; Denisenko, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Belinski, A.; Shatskiy, N.; Chazov, V.; Kuznetsov, A.; Yecheistov, V.; Yurkov, V.; Sergienko, Y.; Varda, D.; Sinyakov, E.; Gabovich, A.; Ivanov, K.; Yazev, S.; Budnev, N.; Konstantinov, E.; Chuvalaev, O.; Poleshchuk, V.; Gress, O.; Frolova, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Parkhomenko, A.; Tlatov, A.; Dormidontov, D.; Senik, V.; Podvorotny, P.; Shumkov, V.; Shurpakov, S.



CV: Hlusko, 18 January 2013, page 1 LESLEA J. HLUSKO  

E-print Network

-2000 PhD, Penn State University, University Park, PA, Biological Anthropology 1996-1998 MA, Penn State University, w/distinction, Univ. Park, PA, Biological Anthropology 1989-1992 BA, University of Virginia, Department of Anthropology Assistant Professor, Program in Ecology and Evolutionary Biology (2003

Hlusko, Leslea J.


CV: Hlusko, 9 January 2012, page 1 LESLEA J. HLUSKO  

E-print Network

-2000 PhD, Penn State University, University Park, PA, Biological Anthropology 1996-1998 MA, Penn State University, w/distinction, Univ. Park, PA, Biological Anthropology 1989-1992 BA, University of Virginia, Department of Anthropology Assistant Professor, Program in Ecology and Evolutionary Biology (2003

Hlusko, Leslea J.


CV of Keivan Esfarjani Page 1 KEIVAN ESFARJANI  

E-print Network

systems. "DEA" (Equivalent to M. Sc.) in Solid-State Physics 86 87 University of Paris 7 and Orsay to ferromagnet transition mechanism. Teaching: electrodynamics, condensed matter physics, solid state physics

Glyde, Henry R.


C. Atsriku_CV1 Christian Atsriku (Ph.D)  

E-print Network

MALDI-LTQ (Thermo Electron), MALDI-TOF (Sciex Voyager DE-STR) Linear ion trap-MS (Thermo Electron LTQ); LC-3D trap of metabolites and dose response studies Provided daily updates and brainstorming on various HCV projects Francisco Project-1: Clinical Proteomic Technology Assessment for Cancer program (CPTAC) funds NCI/NIH Aim

Lawson, Catherine L.


S. Klosterhaus, CV, p. 1 of 3 SUSAN L. KLOSTERHAUS  

E-print Network

(Menlo Park, CA) (invited, 2011) Bay Area Clean Water Agencies, Bay Area Pollution Prevention Group Organic Contaminant Laboratory, University of Maryland, Chesapeake Biological Laboratory, Solomons, MD. HONORS/AWARDS SETAC Chesapeake-Potomac Regional Chapter Student Research Award (2004) Chesapeake


HARVARDgazette15-28 OCTOBER 2009 VOL. CV NO. 4  

E-print Network

hairs, which prompted Boufford's double take when he encountered it in the field. "Why is it so hairy is a dramatic, breathtaking land- scape," Ree said. "You don't ever get used to it." Jack Szostak wins Nobel `It in Pittsburgh? "We don't mind they watch us," said Perez, "because God is watching us." ARTS & CULTURE 7 Hamza

Liu, X. Shirley


Proton CT Setup at CV28 of IEN\\/CNEN  

Microsoft Academic Search

Although the idea of proton Computed Tomography (pCT) is not new, it is currently not available. It is still unknown if the image quality of pCT is sufficient for localizing tumors and if the accuracy of proton range calculations surpass that of X-ray CT based range calculations without significantly increasing the patient exposure. The spatial resolution of pCT is limited

I. Evseev; J. T. Assis; O. Yevseyeva; U. M. Vinagre Filho; J. A. P. Setti; H. R. Schelin; S. A. Paschuk; M. C. L. Klock; R. W. Schulte; R. T. Lopes



CV -P. Malanotte-Rizzoli April 2012 1 CURRICULUM VITAE  

E-print Network

Assembly of the International Union of Geodesy and Geophysics 2005-2008 Member of the Advisory Board (CAB Society 1999-2003 President of the International Association for the Physical Sciences of the Ocean (IAPSO

Seager, Sara


Time-resolved spectroscopy of the pulsating CV GW Lib  

E-print Network

We present time-resolved optical spectroscopy of the dwarf nova GW Librae during its rare April 2007 super-outburst and compare these with quiescent epochs. The data provide the first opportunity to track the evolution of the principal spectral features. In the early stages of the outburst, the optically thick disc dominates the optical and the line components show clear orbital radial velocity excursions. In the course of several weeks, optically thin regions become more prominent as strong emission lines replace the broad disc absorption. Post-outburst spectroscopy covering the I-band illustrates the advantages of CaII relative to the commonly used Balmer lines when attempting to constrain binary parameters. Due to the lower ionisation energy combined with smaller thermal and shear broadening of these lines, a sharp emission component is seen to be moving in between the accretion disc peaks in the CaII line. No such component is visible in the Balmer lines. We interpret this as an emission component origina...

van Spaandonk, L; Marsh, T R; Torres, M A P




E-print Network

ABSTRACTS Vision Sciences Society Meeting, St. Pete Beach, FL 2014 McCarthy, J. D., Barnes, L. N information via color. Vision Sciences Society Meeting, Naples, FL 2013 McCarthy, J. D. & Caplovitz, G. P Neuroscience Society Meeting, San Francisco, CA 2013 McCarthy, J. D., Barnes, L. N. & Caplovitz, G. C. Two plus

Song, Joo-Hyun


Lawrence CV 1 Shawn A. Lawrence, PhD, LCSW  

E-print Network

consumption, tailgating, and negative consequences. Journal of Child & Adolescent Substance Abuse Lawrence, S of Arts in Psychology State University of New York at Buffalo, Buffalo New York Research Focus: Program Evaluation Obesity and Stigmatization in adults and Children Determinants of Physical Activity in Children

Van Stryland, Eric


Lawrence CV 1 Shawn A. Lawrence, PhD, LCSW  

E-print Network

, and negative consequences. Journal of Child & Adolescent Substance Abuse, 21(3), 222-237. DOI: 10.1080/1067828X New York 8/1990-5/1994 Bachelor of Arts in Psychology State University of New York at Buffalo, Buffalo New York Research Focus: Obesity and Stigmatization in Adults and Children Determinants of Physical

Wu, Shin-Tson


CV for Stephen F. Ackley (As of December 2013)  

E-print Network

-Founder of the Joint Cold Regions Science and Engineering Graduate Program between Dartmouth College and USA CRREL University 1991-1996 Research Geophysicist, Snow and Ice Branch, US Army Cold Regions Research Cold Regions Research and Engineering Laboratory, Hanover, NH 1985-1986 Chair in Arctic Marine Science

Texas at San Antonio, University of


Katherine Yanhang Zhang's CV, page 1 Katherine Yanhang Zhang  

E-print Network

of Microelectromechanical System Journal of Nanomaterials Journal of Physics D: Applied Physics Measurement Science and Technology Journal of Applied Physiology Journal of Biomechanical Engineering Journal of Biomechanics Journal 2011 International Mechanical Engineering Congress & Exposition Journal reviewer Acta Mechanica Solida


J.S. Karst CV 1 Jeffrey S. Karst  

E-print Network

Degrees Conferred: Bachelor of Arts, Cum Laude, May 2007 CLINICAL EXPERIENCE and assessments and therapy services with a broad range of children and adults. Therapies include individual psychotherapy, couples counseling, and group therapy drawing

Sanders, Matthew


Temeles, c.v., p. 1 Ethan J. Temeles  

E-print Network

(`98) Susan Darling (`98) Irvin Pan (`99) Amanda Muir (`01) Lauren Peloquin (`02) Elon Slutsky (`02 ('09) Alanna Darling ('09) Shaylon Stolk ('10) Geoffrey Giller ('10) Yoon Rah ('11) Rose Larios ('12

Temeles, Ethan J.


Carelli CV last updated 7/18/101 Curriculum Vitae  

E-print Network

on Drug Abuse. R01: "Neurophysiological Cocaine & Natural Rewards" Competing continuation: Total: $1 and heterogeneity in the nucleus accumbens response to ethanol. Bowles Alcohol Center Grant Pilot Project program Institute on Drug Abuse. R01: "Neurophysiological Study: Cocaine & Natural Rewards" Score: 0.3% Total: $ 1

Carelli, Regina M.


Carelli CV last updated 8/1/121 Curriculum Vitae  

E-print Network

: "Neurophysiological Cocaine & Natural Rewards" Competing continuation: Total: $1,460,000 2004-2014 Principal. Bowles Alcohol Center Grant Pilot Project program. Total: $60,000. 2001-2006 Principal Investigator, National Institute on Drug Abuse. R01: "Neurophysiological Study: Cocaine & Natural Rewards" Score: 0

Carelli, Regina M.


CV Renana Ofan page 1 of 3 Renana H. Ofan  

E-print Network

of undergraduate students. The Belmonte Science Center for Youth 2003 Biology Teacher, 8th grade Physiology class Advisor: Professor Ehud Zohary Studied cortical plasticity in congenitally bilingual blind subjects using and run a behavioral study measuring verbal memory in blind participants. National Herbarium, Israel


Belsky, CV October 2013, p. 1 JILL M. BELSKY  

E-print Network

current projects: one examines the social and ecological significance of historic livelihoods in Bhutan and Conservation Institute in Bumthang, Bhutan. The other examines and compares community forestry in Bhutan

Belsky, Jill M.


Chris Summa. CV. 17 January 2013 1 Christopher M. Summa  

E-print Network

University School of Medicine, CA (USA) NSF Postdoctoral Fellow in Bioinformatics Advisor: Dr. Michael Levitt, Fiebiger E, and Dickinson BL, "Crosstalk between PKA and Epac Signaling Regulates the Functional Maturation

Summa, Christopher M.


________________________________________________________________ F. Ceriotti cv_ceriotti.doc 1/13  

E-print Network

laude). Major Research Interests: Standardisation in clinical chemistry, particularly lipids and enzymes. Evaluation and development of new clinical chemistry tests; differential pH measurement. Quality control technologies in Clinical Chemistry" . Since 1999 he is the Scientific responsible for the External Quality

Rodriguez, Carlos


Unexplained Fe, Ni and S anomalies in CV chondrite components  

NASA Technical Reports Server (NTRS)

Large negative anomalies in Fe, Co, Ni, S and Se are present in Allende Type B Ca-Al-rich inclusions (CAI's). Based on compilations of all the known published analyses, Allende chondrules, aggregates and other types of CAI's also display anomalies. These observations show that: (1) since Fe, Co, Ni, S and Se are more depleted than elements bracketing them in volatility (such as Pt and Au, and Su and Cd), the anomalies were not produced during the volatility dependent, high temperature CAI forming processes; (2) since Pt, Au, Ge and other siderophiles are not as depleted as Fe, Co and Ni, the anomalies are not due to a metal/silicate fractionation; (3) the association of Fe, Co, and Ni anomalies with S and Se anomalies suggests that the anomalies are due to the removal of FeNi sulfide; and (4) since these anomalies occur in all types of Allende inclusions, aggregates and chondrules, the Allende parental material must have undergone sulfide loss before the formation of these components.

Wark, D. A.



Two-page CV, Jan. 2012 Dr. Liping Wei  

E-print Network

and Environmental Engineering, Cornell University, Ithaca, NY M. Sc. 1998 Environmental Chemistry, Nanjing University, Nanjing, China B. Sc. 1995 Environmental Chemistry, Nanjing University, Nanjing, China to the 242nd American Chemical Society the Division of Environmental Chemistry by PhD advisee Varunpreet

Wei, Liping


David Keith, Academic CV (Feb-13) Page 1 David Keith  

E-print Network

of geoengineering. In 1990 I wrote one of the first comparative technology and policy assessments of geoengineering few years I have organized interdisciplinary meetings on geoengineering, created an annual summer


HARVARDgazette15-28 APRIL 2010 VOL. CV NO. 14  

E-print Network

. Editorial Assistant/Sports Alvin Powell Staff Writer Sarah Sweeney Production Assistant/Books Colleen Walsh the importance of public service: "Giving Back: Finding the Best Way to Make a Difference." and greening the campus in the process. Page 6 FROM LAB TRASH TO TREASURE Surplus and waste laboratory equip


HARVARDgazette04-17 MARCH 2010 VOL. CV NO. 11  

E-print Network

around the world to one stage during Cultural Rhythms, an an- nual festival showcasing Harvard's di and staff offices, undergraduate residences, and other locations around the University. U.S. delivery Phonetic Alphabet. ?p=38506 SLAVERY IN 2010 Harvard Kennedy School program looks


SeeingWithOpenCV FollowThatFace!  

E-print Network

,you'd needto decidewhich detectionis the one you're tracking. Finally,if a person's head tilts towards one'sfollowing. The algorithmthat OpenCVuses for face tracking is called Camshift Camshiftuses color information,but ratherthan relyingon a singlecolor,it tracksa combinationof colors.Sinceit tracks by color, it can follow a face

Oh, Paul


Stacie M. King, CV Updated 11-Sep-2013  

E-print Network

, soil chemistry and microscale methods in archaeology Associate, Department of Anthropology 2002-2003 ASSOCIATE FACULTY IN: Ancient Studies Archaeology in Social Context Program Center for Archaeology and the Public Interest Center for Latin American and Caribbean

Scheiber, Laura L.



E-print Network

Reddy. 2003 Studying the effect of vegetation on decomposition of organic matter and greenhouse gas). Alternative pathways for Cr(VI) reduction by consortia of gram positive fermentative bacteria by analyzing

Jawitz, James W.


Highways Traffic Surveillance System (HTSS) using OpenCV  

Microsoft Academic Search

Due to the traffic accidents over the last few years; the development of surveillance systems with multifunctional techniques has received increasing attention. The use of the smart camera is one solution to solve the traffic problems, Smart cameras are cameras that can perform tasks far beyond simply taking photos and recording videos. Highways Traffic Surveillance System (HTSS) is used to

Z. N. K. Wafi; R. B. Ahmad; M. P. Paulraj



James F. Gilchrist cv -page 1/16  

E-print Network

Articles 32) T. Muangnapoh, A. L. Weldon, and J. F. Gilchrist, "Enhanced monolayer deposition via vibration-assisted convective deposition", submitted. 31) B. Xu and J. F. Gilchrist, "Microstructure of sheared monosized colloidal suspensions resulting from hydrodynamic and electrostatic interactions", submitted. 30) X. H. Li

Gilchrist, James F.


CV Science with Athena: A case study of AM Herculis  

NASA Astrophysics Data System (ADS)

The proposed ESA large mission telescope Athena+ has recently been selected for launch in 2028. We investigate the capability of Athena+ with respect to spectral analyses of cataclysmic variables on the case of the prototype of magnetic CVs, AM Herculis. To simulate observations with the X-IFU calorimeter onboard Athena+, we model the emission line spectrum of AM Her as a combination of the multi-temperature plasma emission of the post-shock accretion column and reflection. We generate phase-resolved synthetic X-ray spectra for the models which are sensitive to changes in the accretion rate, the white dwarf mass, and the magnetic field strength. Line shifts due to gas infall in the accretion column, orbital velocity, and gravitational redshift are taken into account. A radial-velocity study of the Fe-line complex at 6.7 keV recovers those velocity components in the H- and He-like plasma lines and the neutral reflection line with 10% accuracy.

Traulsen, I.; Schwope, A.



Karolina Michalska -CV Karolina Michalska, PhD -Curriculum Vitae  

E-print Network

College Advanced Certificate in Protein Crystallography on the Web", 2002/2003 ,,From gene to drug acid bacteria. Acta Cryst. D, accepted 2. K. Michalska, C. Chang, J.C. Mack, S. Zerbs, A. Joachimiak, F

Kemner, Ken


Hudman CV -1/6 Rynda C. Hudman  

E-print Network

transport of pollution over the United States using observations from surface networks and aircraft, aircraft, and satellite air pollutant observations; statistical regressions of meteorological and land effect on forest fires and air quality; satellite monitoring of trace gases. EDUCATION Ph.D. Engineering

Cohen, Ronald C.


Amelia E. Shevenell CV-1 Amelia Endicott Shevenell  

E-print Network

London (2009) · Postgraduate Certificate in Learning and Teaching in Higher Education Ph.D. Marine College, Clinton, New York. EDUCATION Associate Fellow of the Higher Education Academy, University College Fellowship, University of California Santa Barbara (1998-1999) · Antarctic Service Medal (1995, 1998, 2001

Meyers, Steven D.


Vctor Vilarrasa's CV e-mail:  

E-print Network

(UPC), Spain. - Title: Thermo-Hydro-Mechanical Impacts of Carbon Dioxide (CO2) Injection in Deep Saline. Probabilistic Assessment of Groundwater-Related Risks at Subsurface Excavation Sites. Engineering Geology, 125 for Characterizing the Hydraulic Effectiveness of an Annular Low- Permeability Barrier. Engineering Geology, 120, 68

Ajo-Franklin, Jonathan


HARVARDgazette17-30 SEPTEMBER 2009 VOL. CV NO. 2  

E-print Network

REFLECT ON PAST TRIALS, FUTURE DIRECTIONS A conversation between DNA discov- erer James Watson for University Planning and Community Programs Joshua Poupore Associate Communications Officer Perry Hewitt


DISCHER CV Nov. 2013 Page 1 of 25  

E-print Network

Biology Editorial Board (July 2011- 2014) Annual Review Biomedical Engineering ­ Editorial Board 2007, Physics, & Biochemistry PROFESSIONAL EXPERIENCE 12/04 - present Professor, School of Engineering Full Professor at Penn. 5/93 - 12/94 Scientist, Biochemistry/Biophysics, Life Sciences Division, Cell

Discher, Dennis


van de Gevel -CV1 SASKIA L. VAN DE GEVEL  

E-print Network

of Costa Rica, Study Abroad Course - Environmental Geography of the Alps, Environmental Issues, Study Abroad Course - Biogeography of Costa Rica, Environmental Issues in Appalachia of Tennessee. Introduction to the Natural 2005 Environment: Weather, Climate, and Biomes


Near-infrared face recognition utilizing open CV software  

NASA Astrophysics Data System (ADS)

Commercially available hardware, freely available algorithms, and authors' developed software are synergized successfully to detect and recognize subjects in an environment without visible light. This project integrates three major components: an illumination device operating in near infrared (NIR) spectrum, a NIR capable camera and a software algorithm capable of performing image manipulation, facial detection and recognition. Focusing our efforts in the near infrared spectrum allows the low budget system to operate covertly while still allowing for accurate face recognition. In doing so a valuable function has been developed which presents potential benefits in future civilian and military security and surveillance operations.

Sellami, Louiza; Ngo, Hau; Fowler, Chris J.; Kearney, Liam M.



Hirase CV (May 2014) Name: Hajime Hirase, Ph.D.  

E-print Network

: Grant in Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology (Grant #18053026) ¥6 of Education, Culture, Sports, Science and Technology (Grant #21650081) ¥3,100,000 Year 2009-2010 Saitama

Kazama, Hokto


Eastman CV--2-4-14 CURRICULUM VITAE  

E-print Network

in the field, exemplifies the humanistic, philosophical side of my program. My earlier post-tenure article the game theoretic side of my research program together with the humanistic, philosophical side

Lin, Xiaodong


Daniel E. Lieberman CV, pg 1 CURRICULUM VITAE  

E-print Network

Enhancement Fund. The Evolution of Diet in Homo. 1997-1999 National Science Foundation IBN 96-03833. Variation in cortical bone responses to exercise-induced loading 1997-1999 American Federation for Aging Research. Effects of age and exercise on cortical bone growth and porosity 1996-1997 Busch Biomedical Research

Lieberman, Daniel E.


HARVARDgazette15-28 OCTOBER 2009 VOL. CV NO. 4  

E-print Network

Writer Gervis A. Menzies Jr. Editorial Assistant/Sports Alvin Powell Staff Writer Sarah Sweeney Attention: Circulation, Holyoke Center 1060 Cambridge, MA 02138 Periodical postage paid at Boston, MA- sion bombs, is 24,000 years. Ten thousand years is equivalent to 400 generations of human life


CV McPhee-Shaw -1 Curriculum Vitae  

E-print Network

convenor: "Physical, Chemical, and Biological Connections Between Coastal Zones" AGU/ASLO Ocean Sciences California Ocean Observing System. Invited Speaker, IAPSO/SCOR (International Association for the Physical Sciences of the Oceans/ Scientific Committee on Oceanic Research) Conference on Ocean Mixing. Victoria, BC

McPhee-Shaw, Erika


David Erickson, Cornell CV Page 1 Professor David Erickson  

E-print Network

Faculty Award 2007 The DARPA-MTO (Defense Advanced Research Projects Agency ­ Microsystems Technology, Germany July 2002 ­ Aug. 2002 Visiting Scientist Defense Research Establishment Valcartier (DREV) May 1999 of Electrical Engineering: Pasadena, California Post-doctoral Scholar Institute for Polymer Research, Dresden

Erickson, David


Nash CV 1 of 4 Dwayne Abdul Nash, Esq.  

E-print Network

American Studies and Performance Studies Dissertation Title: "Prototypes of Racial Profiling: Forgotten History of Stop and Frisk in 1960s New York" Advisor: Martha Biondi, Ph.D. (Chair) Committee: John Hagan Beautiful Struggle," Northwestern University, Evanston, IL "Forbidden Testimonies of Racial Profiling


Boris Faybishenko's CV January 25, 2010 BORIS FAYBISHENKO  

E-print Network

), Moscow State University of Environmental Engineering, Moscow, Russia. Ph. D (1977), Institute of Hydraulic Engineers and Land Reclamation, Moscow, Russia. B.S. (1970), Hydrogeology and Engineering Geology Laboratory, and Yucca Mountain Nuclear Waste Repository, as well as the DOE International projects--Chernobyl

Ajo-Franklin, Jonathan



E-print Network

antisocial deviance in neurobehavioral terms. Development and Psychopathology, 24, 1047-1071. 7. Moser, J. S). Etiologic relationships between anxiety and