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1

The four INTA-300 rocket prototypes  

NASA Astrophysics Data System (ADS)

A development history and performance capability assessment is presented for the INTA-300 'Flamenco' sounding rocket prototype specimens. The Flamenco is a two-stage solid fuel rocket, based on British sounding rocket technology, that can lift 50 km payloads to altitudes of about 300 km. The flight of the first two prototypes, in 1974 and 1975, pointed to vibration problems which reduced the achievable apogee, and the third prototype's flight was marred by a premature detonation that destroyed the rocket. The fourth Flamenco flight, however, yielded much reliable data.

Calero, J. S.

1985-03-01

2

CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains.  

PubMed

CopraRNA (Comparative prediction algorithm for small RNA targets) is the most recent asset to the Freiburg RNA Tools webserver. It incorporates and extends the functionality of the existing tool IntaRNA (Interacting RNAs) in order to predict targets, interaction domains and consequently the regulatory networks of bacterial small RNA molecules. The CopraRNA prediction results are accompanied by extensive postprocessing methods such as functional enrichment analysis and visualization of interacting regions. Here, we introduce the functionality of the CopraRNA and IntaRNA webservers and give detailed explanations on their postprocessing functionalities. Both tools are freely accessible at http://rna.informatik.uni-freiburg.de. PMID:24838564

Wright, Patrick R; Georg, Jens; Mann, Martin; Sorescu, Dragos A; Richter, Andreas S; Lott, Steffen; Kleinkauf, Robert; Hess, Wolfgang R; Backofen, Rolf

2014-07-01

3

Retrospective study of bovine neonatal mortality: cases reported from INTA Balcarce, Argentina  

Microsoft Academic Search

A retrospective study was performed on 169 beef and dairy calves aged from 1 to 7 days old submitted to the Diagnostic Laboratories at INTA Balcarce, Argentina. Bacterial culture was performed for aerobic and microaerophilic organisms. Samples from spleen and lymph nodes, and peripheral blood mononuclear cells were also cultured for viral isolation on cell culture. Bovine rotavirus was detected

E. L. MORRELL; D. P. MOORE; M. A. POSO; E. ODRIOZOLA; G. CANTÓN; F. PAOLICCHI; R. MALENA; M. R. LEUNDA; C. MORSELLA; C. M. CAMPERO

4

Photometric follow-up of transiting exoplanets with the INTA-CAB robotic telescope  

NASA Astrophysics Data System (ADS)

We present some photometric results obtained by our group as part of a photometric follow-up of transiting exoplanets carried out with the INTA-CAB 50-cm robotic telescope at Calar Alto Observatory (Almería, Spain). We show light curves of WASP-10, HATP-20 and HATP-32 and, after that, we fit our results with theoretical models in order to obtain some parameters as the central times of transits, depths and the transit durations of this extrasolar planets.

Ullán, A.; Eibe, M. T.; Cuesta, L.; Pérez-Verde, A.; Navas, J.

2013-05-01

5

Levels of job satisfaction and role perceptions held by extension agents of INTA'S Regional Parana in Argentina  

E-print Network

or Open-Ended Questions 72 Job Satisfaction as Revealed by Semanti Dif- ferentials To Be an Extension Agent Is. . . INTA as a Government Entity Zs. . . The Audience of My Extension Agency Zs. . Summary of Extension Agents' Reactions tc Scales 83 83...LEVELS OF JOB SATISFACTION AND ROLE PERCEPTIONS BELD BY EXTENSION AGENTS OF INTA'S REGIONAL PARANA ZN ARGENTINA A Thesis FRANCISCO JOSE BLAHA Submitted to the Graduate College of Texas A6N University in partial fulfillment of the requirements...

Blaha, Francisco Jose

1983-01-01

6

Retrospective study of bovine neonatal mortality: cases reported from INTA Balcarce, Argentina.  

PubMed

A retrospective study was performed on 169 beef and dairy calves aged from 1 to 7 days old submitted to the Diagnostic Laboratories at INTA Balcarce, Argentina. Bacterial culture was performed for aerobic and microaerophilic organisms. Samples from spleen and lymph nodes, and peripheral blood mononuclear cells were also cultured for viral isolation on cell culture. Bovine rotavirus was detected by direct-ELISA. Multiple tissue samples were fixed in 10% formalin, routinely processed and Stained with hematoxylin and eosin for microscopic examination. Etiological diagnosis was made in 70 of the 169 calves. Infectious agents were identified in 49 cases, the most common being Escherichia coli. When the histopathological examination was performed in cases with undetermined diagnosis, it was noted that 44 specimens had histological lesions, which suggested the presence of an infectious agent. In order to characterize the causes of bovine neonatal mortality, the protocols and methodology should be improved in further works. PMID:19024501

Morrell, E L; Moore, D P; Odeón, A C; Poso, M A; Odriozola, E; Cantón, G; Paolicchi, F; Malena, R; Leunda, M R; Morsella, C; Campero, C M

2008-01-01

7

End-to-end validation process for the INTA-Nanosat-1B Attitude Control System  

NASA Astrophysics Data System (ADS)

This paper describes the end-to-end validation process for the Attitude Control Subsystem (ACS) of the satellite INTA-NanoSat-1B (NS-1B). This satellite was launched on July 2009 and it has been fully operative since then. The development of its ACS modules required an exhaustive integration and a system-level validation program. Some of the tests were centred on the validation of the drivers of sensors and actuators and were carried out over the flying model of the satellite. Others, more complex, constituted end-to-end tests where the concurrency of modules, the real-time control requirements and even the well-formedness of the telemetry data were verified. This work presents an incremental and highly automatised way for performing the ACS validation program based on two development suites and an end-to-end validation environment. The validation environment combines a Flat Satellite (FlatSat) configuration and a real-time emulator working in closed-loop. The FlatSat is built using the NS-1B Qualification Model (QM) hardware and it can run a complete version of the on-board software with the ACS modules fully integrated. The real-time emulator, running on an industrial PC, samples the actuation signals and emulates the sensors signals to close the control loop with the FlatSat. This validation environment constitutes a low-cost alternative to the classical three axes tilt table, with the advantage of being easily configured for working under specific orbit conditions, in accordance with any of the selected tests. The approach has been successfully applied to the NS-1B in order to verify different ACS modes under multiple orbit scenarios, providing an exhaustive coverage and reducing the risk of eventual errors during the satellite's lifetime. The strategy was applied also during the validation of the maintenance and reconfiguration procedures required once the satellite was launched. This paper describes in detail the complete ACS validation process that was performed and it shows the most relevant errors detected and fixed during testing. Finally it summarises some of the most significant conclusions.

Polo, Óscar R.; Esteban, Segundo; Cercos, Lorenzo; Parra, Pablo; Angulo, Manuel

2014-01-01

8

CV Surgery Online  

NSDL National Science Digital Library

Stanford University's HighWire Press has announced the online publication of several journals. CV Surgery Online offers "a fully searchable online compilation of articles pertinent to the cardiothoracic and vascular surgeon from the 5 American Heart Association Journals: Circulation, Circulation Research, Hypertension, Stroke, and Arteriosclerosis, Thrombosis, and Vascular Surgery." Published by the American Heart Association in conjunction with HighWire Press, the free trial period for CV Surgery Online ended December 31, 2000.

9

MASTER: CV, DNe, variables.  

NASA Astrophysics Data System (ADS)

MASTER OT J105529.06-255654.5 discovery - new CV MASTER-SAAO auto-detection system ( Lipunov et al., Advances in Astronomy, MASTER Global Robotic Net, 2010 ) discovered OT source at (RA, Dec) = 10h 55m 29.06s -25d 56m 54.5s on 2015-03-11.89942 UT.

Balanutsa, P.; Gress, O.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Antipin, S.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Chazov, V.; Vladimirov, V.; Kuvshinov, D.; Shurpakov, S.; Shumkov, V.; Potter, S.; Kniazev, A.; Kotze, M.; Gress, O.; Ivanov, K.; Budnev, N.; Yazev, S.; Poleshchuk, V.; Konstantinov, E.; Chuvalaev, O.; Tlatov, A.; Dormidontov, D.; Senik, V.; Parkhomenko, A.; Yurkov, V.; Sergienko, Y.; Gabovich, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.

2015-03-01

10

CV-990 LSRA  

NASA Technical Reports Server (NTRS)

A NASA CV-990, modified as a Landing Systems Research Aircraft (LSRA), is serviced on the ramp at NASA's Dryden Flight Research Center, Edwards, California, before a test of the space shuttle landing gear system. The space shuttle landing gear test unit, operated by a high-pressure hydraulic system, allowed engineers to assess and document the performance of space shuttle main and nose landing gear systems, tires and wheel assemblies, plus braking and nose wheel steering performance. The series of 155 test missions for the space shuttle program provided extensive data about the life and endurance of the shuttle tire systems and helped raise the shuttle crosswind landing limits at Kennedy.

1992-01-01

11

CV 100--Still Going Strong  

ERIC Educational Resources Information Center

Describes results of a study that used CV 100, a fuel additive for use in oil-fired heating systems, on a trial basis in 12 Ontario schools. The test showed an average 12 percent reduction in fuel costs in the schools using CV 100. (JG)

Abrahams, A. L.

1976-01-01

12

Year Action IB # Jig HAL CP HAL CV Trawl CP Trawl CV  

E-print Network

Year Action IB # Jig Pot Gear (CP and CV) HAL CP HAL CV Trawl CP Trawl CV TOTAL TAC ABC OFL 396 5,841 Year Action IB # Jig Pot Gear (CV and CP) HAL CP HAL CV 50 HAL CV ,408 NOTES: IB = Information Bulletin TAC = Total Allowable Catch ABC = Acceptable Biological Catch OFL

13

Digitization, Integration and Preservation of Technical and Historical Information: The Case of INTA, the National Institute for Aerospace Technique of Spain  

NASA Astrophysics Data System (ADS)

During the last two years the INTA -- National Institute for Aerospace Technique -- library has been improving different areas related to the information management processes, such as those related to cataloguing, dissemination of technical information, centralization at the Library of all relevant documents and information applicable to scientific research within our organization, implementation of library web services, etc. As part of these processes of modernization of services that the INTA Library is carrying out, a project of digitization of both technical documentation and historical records of the Institute has been defined. The goal is to achieve the total digitization of technical documents and historical papers through the year 2006, and provide access for the resulting electronic collection to the Spanish aerospace community. For the development of the project a deep study of the state of the art in digitization and preservation matters has been conducted. That study covers the different aspects of such a project that could be experienced, such as the risk of data loss, the bandwidth needed to guarantee access to this huge quantity of electronic documentation, the fragility of the digital media, the rapid obsolescence of hardware and software, etc. Also the project is going to assume the new reality of documents that are not originating in paper format, but are digital-born, and how to integrate all the electronic documents in one system, fulfilling the same standards and using the same available technology.

Merida Martín, F.; Paz Otero, S.

2007-10-01

14

Master-Saao new CV  

NASA Astrophysics Data System (ADS)

MASTER OT J014522.24-280325.6 - new CV MASTER-SAAO auto-detection system ( Lipunov et al., MASTER Global Robotic Net, Advances in Astronomy, 2010 ) discovered OT source at (RA, Dec) = 01h 45m 22.24s -28d 03m 25.6s on 2015-02-13.80071 UT.

Balanutsa, P.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Kornilov, V.; Kuznetsov, A.; Tiurina, N.; Chazov, V.; Potter, S.; Kniazev, A.; Kotze, M.; Gress, O.; Ivanov, K.; Budnev, N.; Yazev, S.; Konstantinov, E.; Chuvalaev, O.; Poleshchuk, V.; Tlatov, A.; Dormidontov, D.; Senik, V.; Parkhomenko, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Yurkov, V.; Sergienko, Y.; Gabovich, A.; Shumkov, V.; Shurpakov, S.

2015-02-01

15

Open CV intro Aside: Histogram Equalization  

E-print Network

Open CV intro Aside: Histogram Equalization Images are from Wikipedia. #12;Today we'll code: A fully functional sparse optical flow algorithm! Plan ·! OpenCV Basics ­! What is it? ­! How do you get started with it? ·! Feature Finding and Optical Flow ­! A brief mathematical discussion. ·! OpenCV

Kosecka, Jana

16

Introduction to OpenCV David Stavens  

E-print Network

1 Introduction to OpenCV David Stavens Stanford Artificial Intelligence Lab Tonight we'll code extensively in the 223b competition.) Plan OpenCV Basics What is it? How do you get started with it? Feature Finding and Optical Flow A brief mathematical discussion. OpenCV Implementation of Optical Flow Step

Thrun, Sebastian

17

CV  

E-print Network

Aug 14, 2014 ... Rand, D.G., Zhou, Q., Buzzard, G.T., and Fox, J.J., Computationally efficient strategy for modeling ... doi: 10.1016/j.aca.2012.10.005. 36. Buzzard, G.T., Efficient basis ... Proceeding, O'Farrell Street, San Francisco, CA, 2011. 3.

2014-08-14

18

CV  

E-print Network

My interests in biology, health, and medicine, combined with my fascination with ... research has been mathematical models in cancer and infectious diseases, ... Education. • Ph.D. in Mathematics, Vanderbilt University, Nashville, TN, 2012.

2013-09-23

19

CV  

E-print Network

Mar 31, 2015 ... Rand, D.G., Zhou, Q., Buzzard, G.T., and Fox, J.J., Computationally efficient strategy for modeling ... doi: 10.1016/j.aca.2012.10.005. 36. Buzzard, G.T., Efficient basis ... Proceeding, O'Farrell Street, San Francisco, CA, 2011. 3.

2015-03-31

20

CV  

E-print Network

October 2014. Current Position ... NSF Research Grant “Boundary Effects in Critical Phenomena” as PI ($267,705) ... 30th International Colloquium on Group Theoretical Methods .... Referee for “Journal of Physics A”,“Physics Letters A” and

2014-10-29

21

Jared W. Young, CV. CURRICULUM VITAE  

E-print Network

, Department of Psychobiology EDUCATIONAL HISTORY 2001 ­ 2005: Ph.D. in Psychopharmacology, Department-clinical psychopharmacology: British Association for Psychopharmacology 2009: Travel Award: American College of Neuropsychopharmacology #12;Jared W. Young, CV. 2 2008: Outstanding poster presentation in preclinical psychopharmacology

Squire, Larry R.

22

[Application of Tiantu (CV 22) in emergency].  

PubMed

The operation and application of Tiantu (CV 22) in emergency are discussed in the paper. The stimulating methods at Tiantu (CV 22) are acupuncture and pressing technique. The correct insertion of needle and proper depth and direction of insertion are required during acupuncture. The pressing technique stress the pressing strength and pressing time. Acupuncture or pressing technique is suitable for the emergent measurement of asthma, asthmatic breathing, coma, blockage of phlegm, hiccup, sore throat, etc. It is indicated that Tiantu (CV 22) is the key point in the emergency and phlegm resolving. Based on the characteristics of the point as promoting qi circulation, reducing the reversed qi and resolving phlegm, in light of the proper points combination by different syndromes and in terms of the correct and safe stimulating methods, Tiantu (CV 22) can achieve the immediate therapeutic effects in the emergent situations. PMID:23967641

Zhu, Xian-Min; Huo, Shang-Fei; Lu, Lu; Wang, Xin-Zhi

2013-06-01

23

Complete sequence of three plasmids from Bacillus thuringiensis INTA-FR7-4 environmental isolate and comparison with related plasmids from the Bacillus cereus group.  

PubMed

Bacillus thuringiensis is an insect pathogen used worldwide as a bioinsecticide. It belongs to the Bacillus cereus sensu lato group as well as Bacillus anthracis and B. cereus. Plasmids from this group of organisms have been implicated in pathogenicity as they carry the genes responsible for different types of diseases that affect mammals and insects. Some plasmids, like pAW63 and pBT9727, encode a functional conjugation machinery allowing them to be transferred to a recipient cell. They also share extensive homology with the non-functional conjugation apparatus of pXO2 from B. anthracis. In this study we report the complete sequence of three plasmids from an environmental B. thuringiensis isolate from Argentina, obtained by a shotgun sequencing method. We obtained the complete nucleotide sequence of plasmids pFR12 (12,095bp), pFR12.5 (12,459bp) and pFR55 (55,712bp) from B. thuringiensis INTA-FR7-4. pFR12 and pFR12.5 were classified as cryptic as they do not code for any obvious functions besides replication and mobilization. Both small plasmids were classified as RCR plasmids due to similarities with the replicases they encode. Plasmid pFR55 showed a structural organization similar to that observed for plasmids pAW63, pBT9727 and pXO2. pFR55 also shares a tra region with these plasmids, containing genes related to T4SS and conjugation. A comparison between pFR55 and conjugative plasmids led to the postulation that pFR55 is a conjugative plasmid. Genes related to replication functions in pFR55 are different to those described for plasmids with known complete sequences. pFR55 is the first completely sequenced plasmid with a replication machinery related to that of ori44. The analysis of the complete sequence of plasmids from an environmental isolate of B. thuringiensis permitted the identification of a near complete conjugation apparatus in pFR55, resembling those of plasmids pAW63, pBT9727 and pXO2. The availability of this sequence is a step forward in the study of the molecular basis of the conjugative process in Gram positive bacteria, particularly due to the similarity with known conjugation systems. It is also a contribution to the expansion of the non-pathogenic B. cereus plasmid gene pool. PMID:19654019

Amadio, Ariel F; Benintende, Graciela B; Zandomeni, Rubén O

2009-11-01

24

SeeingWithOPenCV FindingFacesin lmages  

E-print Network

SeeingWithOPenCV FindingFacesin lmages what this mouthfulmeans.Figure1 showsan exampleof OPenCV severalscales. The classifierusesdata storedin an XMLfileto decidehow to classify each image location.The OPenCV andinstallingOpenCV,readingand writingimagefiles,capturingvideo, andworkingwiththelPllmagedata structufe. r

Oh, Paul

25

Tutorials on Matlab, OpenCV, and Computer Science Department  

E-print Network

Tutorials on Matlab, OpenCV, and SDL Computer Science Department Hao Jiang #12;Matlab · Matlab. · Rewrite bottleneck procedures using c. ­ System(`c_exe'); ­ Compile the function using mex. #12;OpenCV #12;OpenCV · OpenCV is a C library for image processing and computer vision. http

Jiang, Hao

26

SegCV : traitement efficace de CV avec analyse et correction d'erreurs  

E-print Network

sur une grande collection de CV en français avec correction du bruit montrent de bons résultats en, Ressources humaines, traitement de CV, Modèle à base de règles. KEYWORDS: Information Retrieval, Human

27

Hynek CV, Page 1 CURRICULUM VITAE  

E-print Network

has focused on the geologic, geochemical, hydrologic, and climatic evolutions of the planet Mars. He properties of Mars, impact craters, and the physics of volcanic eruptions on Earth and other planets. Brian time on Mars and determine the history of water and volcanism on the Red Planet. #12;Hynek CV, Page 2

Mojzsis, Stephen J.

28

CV De Juan Vigaray CURRICULUM VITAE  

E-print Network

by the Market Team, Barcelona (Spain): "Retail Sales Promotion & Merchandising". Fall 1994 Teaching AssistantCV De Juan Vigaray 1 CURRICULUM VITAE María D. De Juan Vigaray, PhD RESEARCH INTERESTS TEACHING Spain Phone: + 00 34 9 6 590 34 00 (ext. 3167) Fax: + 00 34 (9) 6 512 61 20 E-mail: mayo

Escolano, Francisco

29

CV (Mercado). February 2005 NORMAN MERCADO SILVA  

E-print Network

CV (Mercado). February 2005 NORMAN MERCADO SILVA Center for Limnology University of Wisconsin - Madison; 2001. PhD: Zoology and Limnology, Department of Zoology, University of Wisconsin ­ Madison, 2005 - Postdoctoral Associate. Center for Limnology (University of Wisconsin - Madison) 2004 - 05 Research Assistant

Mercado-Silva, Norman

30

The Limbic-Prefrontal Network Modulated by Electroacupuncture at CV4 and CV12  

PubMed Central

fMRI studies showed that acupuncture could induce hemodynamic changes in brain networks. Many of these studies focused on whether specific acupoints could activate specific brain regions and were often limited to manual acupuncture at acupoints on the limbs. In this fMRI study, we investigated acupuncture's modulation effects on brain functional networks by electroacupuncture (EA) at acupoints on the midline of abdomen. Acupoints Guanyuan (CV4) and Zhongwan (CV12) were stimulated in 21 healthy volunteers. The needling sensations, brain activation, and functional connectivity were studied. We found that the limbic-prefrontal functional network was deactivated by EA at CV4 and CV12. More importantly, the local functional connectivity was significantly changed during EA stimulation, and the change persisted during the period after the stimulation. Although minor differences existed, both acupoints similarly modulated the limbic-prefrontal functional network, which is overlapped with the functional circuits associated with emotional and cognitive regulation. PMID:22291848

Fang, Jiliang; Wang, Xiaoling; Liu, Hesheng; Wang, Yin; Zhou, Kehua; Hong, Yang; Liu, Jun; Wang, Lei; Xue, Chao; Song, Ming; Liu, Baoyan; Zhu, Bing

2012-01-01

31

SeeingWithOpenCV FollowThatFace!  

E-print Network

SeeingWithOpenCV FollowThatFace! describedin lastmonth'sarticle.To do that, however,you'd needto'dnepdto handlethatJ v t v u situation,aswell. Fortunately,OpenCV includes specializedcode for trackinga faceCVtotrackataceonce you'vedetectedit. FaceTrackingin OpenCV Trackinga face is more drfficult thantrackinga strongly

Oh, Paul

32

The OpenCV Library: Computing Optical Flow  

E-print Network

1 The OpenCV Library: Computing Optical Flow David Stavens Stanford Artificial Intelligence Lab I optical flow algorithm! Plan OpenCV Basics What is it? How do we get started? Feature Finding and Optical Flow A brief mathematical discussion. OpenCV Implementation of Optical Flow Step by step. #12;3 What

Thrun, Sebastian

33

Video Encryption Based on OpenCV  

Microsoft Academic Search

This paper uses the open source computer vision library (OpenCV for short) as basic library and calls library functions to achieve real-time video capture and output. We narrate the process of making the matrix interpretable as an image through analyzing the data structure of IplImage and meaning of its member variable. The library functions to read video take the video

Cunyan Meng; Xinghui Zhang

2010-01-01

34

EARTH, MOON, SUN, AND CV ACCRETION DISKS  

SciTech Connect

Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if accretion disks are present or not. Our results suggest that the accretion disk's geometric shape directly affects the disk's precession rate.

Montgomery, M. M. [Department of Physics, University of Central Florida, Orlando, FL 32816 (United States)

2009-11-01

35

Ch OpenCV for interactive open architecture computer vision  

Microsoft Academic Search

In this paper, design and implementation of an interactive open architecture computer vision software package called Ch OpenCV is presented. Benefiting from both Ch and OpenCV, Ch OpenCV has many salient features. It is interactive, capable of interface with binary static or dynamical C\\/C++ libraries, integrated with advanced numerical features and embeddable. It is especially suitable for rapid prototyping, web-based

Qingcang Yu; Harry H. Cheng; Wayne W. Cheng; Xiaodong Zhou

2004-01-01

36

MIZEX, 1984, NASA CV-990 flight report  

NASA Technical Reports Server (NTRS)

During June/July 1984, the NASA CV-990 Airborne Laboratory was utilized in a mission to overly the Fram Strait/East Greenland Sea marginal ice zone (MIZ) during the main summer marginal ice zone experiment (MIZEX '84). The eight data flights were coordinated where possible with overpasses of the Nimbus-7 satellite, and with measurement of sea ice, open ocean, and atmospheric properties at the surface. The surface research teams were based on seven research vessels, some with helicopters: (1) M/V Kvitbjorn, (2) M/V Polarqueen; (3) M/S Haakon Mosby; (4) a M/S H.U. Sverdrup, all from Norway; (5) F/S Polarstern from the Federal Republic of Germany; and (6) the USNS Lynch from the USA. There were also coordinated flights with the NRL P3, NOAA P3, Canadian CV580, and the French B-17 during the overlap portions of their respective missions. Analysis of the real-time data acquired during the mission and uncalibrated data stored on tape has served to indicate the mission was over 90% successful.

1985-01-01

37

K-Band Observations of Subgap CV's  

NASA Astrophysics Data System (ADS)

We present the continuing results of VLT ISAAC K-band spectroscopy of short period cataclysmic variable (CV) systems below the "period gap” between 2 to 3 hours. It is extremely difficult to see the secondary stars in short period systems, since the low luminosity secondaries are swamped by the accretion disks in these objects. We show the infrared spectra for 9 systems below the gap: V2051 Oph, V436 Cen, EX Hya, VW Hyi, Z Cha, WX Hyi, V893 Sco, RZ Leo, and TY PsA. We are able to clearly detect the secondary stars in all but WX Hyi, V893 Sco, and TY PsA. We present the first direct detections of the secondary stars of V2051 Oph and V436 Cen, and present new detections for EX Hya, VW Hyi, Z Cha, and RZ Leo. Previous infrared spectroscopic surveys of CVs above the period gap reveal that these secondaries suffer a universal deficit of C12, enhanced levels of C13, and unusual abundance patterns for other species (e.g., Mg,Si, Al, Ca). Infrared spectroscopy of short period magnetic CVs (polars) have shown that their secondary stars appear completely normal, and the secondaries in a sample of "pre-CVs" have normal carbon abundances. Implications for CV evolution and formation scenarios will be discussed.

Hamilton, Ryan T.; Harrison, T. E.; Tappert, C.; Howell, S. B.

2010-01-01

38

Characterization of a protein tyrosine phosphatase gene CvBV202 from Cotesia vestalis polydnavirus (CvBV).  

PubMed

Cotesia vestalis (Haliday) (Hymenoptera: Braconidae) is an endoparasitoid of the larval stage of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) and injects a polydnavirus (CvBV) into its host during oviposition. In this paper we characterize CvBV202 and its product. CvBV202 is located on segment S2 of CvBV genome; it has a size of 984 bp and encodes a putative protein of 328 amino acids, including protein phosphatase domain and tyrosine-specific protein phosphatase domain. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected for 6 days. Tissue-specific patterns of this gene expression showed that CvBV202 had a close relationship with the host's physiological alternations including immunosuppression, modulation of hormone titer, and nutrition metabolism. The protein was detected in the parasitized hosts at 12 h p.p. using western blot assay. The product of CvBV202 was found to be around 59 kDa, much larger than the predicted molecular weight of 37.8 kDa, suggesting that post-translational modification of CvBV202 occurs in host cells, corresponding with the existence of many post-translational modification sites. Immunofluorescence staining and confocal laser scanning microscopy revealed that CvBV202 and the fused protein eGFP-CvBV202 were observed both in the nuclear region and cytoplasm of the hemocytes of the naturally parasitized host larvae and rBac-eGFP-CvBV202-infected Tn-5B1-4 cells, respectively. PMID:18425572

Shi, Min; Chen, Ya-Feng; Yao, Yuan; Huang, Fang; Chen, Xue-Xin

2008-06-01

39

Micropropagation of sour cherry ( Prunus cerasus L.) cv. Šumadinka  

Microsoft Academic Search

Sour cherry cv. Šumadinka (Prunus cerasus L.) is the leading Yugoslav cultivar for production orchards. A method of micropropagation has been developed for the purpose of growing ‘Šumadinka’ on its own roots and for rapid multiplication.

Radosav Cerovi?; Djurdina Ruži?

1987-01-01

40

Axel Jantsch -Complete CV 1 Personal facts 2  

E-print Network

Axel Jantsch - Complete CV June 2013 Contents 1 Personal facts 2 2 Research interests 2 3 Education.3 Leadership in research and education programs . . . . . . . . . . . . . . . . . . 4 4.4 Industrial positions Education 16 9.1 Course responsible . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 9

Jantsch, Axel

41

Jason Aughenbaugh: CV Page 1 JASON MATTHEW AUGHENBAUGH  

E-print Network

Jason Aughenbaugh: CV Page 1 JASON MATTHEW AUGHENBAUGH CURRICULUM VITAE SUMMARY OF INTERESTS applications in reliability analysis of dynamic systems, risk-based design, simulation- based design, sensor.D. 2006 Mechanical Engineering Georgia Institute of Technology Dissertation: Managing uncertainty

42

Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae  

E-print Network

Finkel CV, Page 1 Eli J. Finkel ­ Curriculum Vitae (November, 2014) Professional Information Mail://faculty.wcas.northwestern.edu/ http://www.kellogg.northwestern.edu/ eli-finkel/ faculty/directory/ finkel_eli.aspx Education Ph

Reber, Paul J.

43

Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae  

E-print Network

Finkel CV, Page 1 Eli J. Finkel ­ Curriculum Vitae (July, 2014) Professional Information Mail://faculty.wcas.northwestern.edu/ http://www.kellogg.northwestern.edu/ eli-finkel/ faculty/directory/ finkel_eli.aspx Education Ph

Reber, Paul J.

44

van de Gevel -CV1 SASKIA L. VAN DE GEVEL  

E-print Network

of Costa Rica, Senior Seminar Capstone Course, Environmental Issues in Appalachia Course - Biogeography of Costa Rica, Environmental Issues in Appalachia, Introduction to the Natural 2005 Environment: Weather, Climate, and Biomes. #12;van de Gevel - CV2 Head

Thaxton, Christopher S.

45

ASAS-SN Discovery of a Likely CV  

NASA Astrophysics Data System (ADS)

During the ongoing All Sky Automated Survey for SuperNovae (ASAS-SN or "Assassin"), using data from the quadruple 14-cm "Brutus" telescope in Haleakala, Hawaii, we discovered a new transient source, likely a CV.

Davis, A. B.; Shappee, Bart; Shappee, B. J.; Will, D.; Simonian, G.; Stanek, K. Z.; Holoien, T. W.-S.; Kochanek, C. S.; Basu, U.; Gross, N.; Beacom, J. F.; Prieto, J. L.; Bersier, D.; Brimacombe, J.; Dong, Subo; Wozniak, P. R.; Szczygiel, D.; Pojmanski, G.

2014-12-01

46

Insights from High Precision Oxygen Isotopic Analyses of Reduced CV Meteorite Separates: CV Matrix Homogeneity and Preservation of Nebular Heterogeneity  

NASA Astrophysics Data System (ADS)

The oxygen-isotopic composition of matrix from reduced CVs Efremovka and Vigarano has been measured and is indistinguishable from Allende. This indicates a similar nebular history and a preservation of different nebular conditions within reduced CV meteorites.

Dyl, K. A.; Young, E. D.

2011-03-01

47

Ch OpenCV for interactive open architecture computer vision Qingcang Yua  

E-print Network

Ch OpenCV for interactive open architecture computer vision Qingcang Yua , Harry H. Chenga,*, Wayne and implementation of an interactive open architecture computer vision software package called Ch OpenCV is presented. Benefiting from both Ch and OpenCV, Ch OpenCV has many salient features. It is interactive, capable

Cheng, Harry H.

48

Opaque Assemblages in CK and CV Carbonaceous Chondrites  

NASA Technical Reports Server (NTRS)

CK carbonaceous chondrites are the only group of carbonaceous chondrites that exhibit thermal metamorphism. As a result, CKs display features of metamorphism such as silicate darkening, recrystallization and shock veins. Calcium Aluminum Inclusions and Fe-Ni metal are rare. CV carbonaceous chondrites are unequilibrated and have two subgroups; oxidized and reduced. The CV and CK carbonaceous chondrite groups have been compared to each other often because of petrographic similarities, such as overlapping oxygen isotopic ratios. Scientists have suggested the two groups of carbonaceous chondrites formed from the same parent body and CKs are equilibrated CV chondrites [1, 2]. The oxidized CV group has been most closely related to CKs. This study examines the petrology and mineralogy of CKs and CVs focusing on opaque minerals found in the meteorites. Using the oxide, metal and sulfide assemblages, constraints can be placed on the temperature and oxygen fugacity at which the meteorites equilibrated. The temperature and oxygen fugacity of the CK and CV chondrites can be compared in order to help define their formation history.

Neff, K. E.; Righter, K.

2006-01-01

49

CV of BIN LIU Page 1 of 5  

E-print Network

CV of BIN LIU Page 1 of 5 Curriculum Vitae BIN LIU, Ph.D. CURRENT AFFILIATION Research Assistant-12. 5. Li, S., D. Zhao, L. Zhou, and B. Liu, 2013: Dependence of mean square slope on wave state and its. Liu, B., C. Guan, L. Xie, and D. Zhao, 2012: An Investigation of the effects of wave state and sea

Parker, Matthew D. Brown

50

R. MICHAEL YOUNG CV MAY 2014 R. Michael Young  

E-print Network

!1 R. MICHAEL YOUNG CV MAY 2014 R. Michael Young Professor Department of Computer Science Campus://liquidnarrative.csc.ncsu.edu/rmy/ ! BIOGRAPHIC SUMMARY ! Michael Young is a Professor of Computer Science at North Carolina State University was among the first researchers to develop models of planning adapted for story generation and leveraged

Young, R. Michael

51

Computational simulation of CV combination preferences in babbling  

PubMed Central

There is a tendency for spoken consonant-vowel (CV) syllables, in babbling in particular, to show preferred combinations: labial consonants with central vowels, alveolars with front, and velars with back. This pattern was first described by MacNeilage and Davis, who found the evidence compatible with their “frame-then-content” (F/C) model. F/C postulates that CV syllables in babbling are produced with no control of the tongue (and therefore effectively random tongue positions) but systematic oscillation of the jaw. Articulatory Phonology (AP; Browman & Goldstein) predicts that CV preferences will depend on the degree of synergy of tongue movements for the C and V. We present computational modeling of both accounts using articulatory synthesis. Simulations found better correlations between patterns in babbling and the AP account than with the F/C model. These results indicate that the underlying assumptions of the F/C model are not supported and that the AP account provides a better and account with broader coverage by showing that articulatory synergies influence all CV syllables, not just the most common ones. PMID:24496111

Nam, Hosung; Goldstein, Louis M.; Giulivi, Sara; Levitt, Andrea G.; Whalen, D. H.

2013-01-01

52

A Charge Balanced CV Converter for a Differential Capacitance Sensor  

Microsoft Academic Search

This paper presents a simple new configuration for a switched capacitor type C-V converter based on a charge balance principle for a differential capacitance sensor and its simulation results. The new converter was devised and realized using only one operational amplifier, nine CMOS switches and an oscillation circuit. It was shown that the offset output due to the input offset

Masahiro Tsugai; Yoshiaki Hirata; Toru Araki; Masafumi Kimata

2003-01-01

53

HARVARDgazette04-17 FEBRUARY 2010 VOL. CV NO. 9  

E-print Network

HARVARDgazette04-17 FEBRUARY 2010 VOL. CV NO. 9 NEWS.HARVARD.EDU/GAZETTE Athletics for all Cambridge, MA 02138 Periodical postage paid at Boston, MA. Harvard Gazette (issn: 0364-7692) is published Square before being lovingly roasted inside New College Theatre. Man of the Year Justin Timberlake

54

Effects of paclobutrazol on avocado (Persea americana Mill.) cv. ‘Fuerte’  

Microsoft Academic Search

Adato, I., 1990. Effects of paclobutrazol on avocado (Persea americana Mill. ) cv. 'Fuerte'. Scientia Hortic., 45:105-115. Mature avocado trees (Persea americana Mill. ) of cultivar 'Fuerte' were sprayed with Cultar ~, a commercial formulation containing 25% paclobutrazol (ICI, U.K.). Hand spraying of individual trees before or at the flowering stage resulted in an increase in the number of harvested

I. Adato

1990-01-01

55

Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae  

E-print Network

Finkel CV, Page 1 Eli J. Finkel ­ Curriculum Vitae (October, 2013) Professional Information Mail@kellogg.northwestern.edu Numbers: Phone: 847-491-3212 / Fax: 847-491-7859 TBD Psych Website: faculty.wcas.northwestern.edu/eli-finkel/ MORS Website: http://www.kellogg.northwestern.edu/faculty/directory/finkel_eli.aspx Education Ph

Reber, Paul J.

56

CV, T.S. Hopkins, 2009 Thomas Sawyer HOPKINS  

E-print Network

CV, T.S. Hopkins, 2009 1 Thomas Sawyer HOPKINS 288 Jefferson Rd, Princeton, NJ, USA Date of Birth 27695 Home: 288 Jefferson Rd. Princeton NJ 08540, USA email: tom_hopkins@ncsu.edu CNR/IAMC - Institute 3811, 3817 Fax:+39 081-542 3888 email: tom.hopkins@iamc.cnr.it EDUCATION Degree Institution Conferring

Parker, Matthew D. Brown

57

Segmentation of kidney using CV model and anatomy priors  

Microsoft Academic Search

This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and

Jinghua Lu; Jie Chen; Juan Zhang; Wenjia Yang

2007-01-01

58

Segmentação, Indexação e Recuperação de Vídeo Utilizando OpenCV  

Microsoft Academic Search

Resumo: Os constantes avanços em poder de processamento, capacidade de armazenamento, e largura de banda tornaram possível ao usuário de com- putadores o acesso, manipulação e edição de imagens e vídeos digitais. Nesse tutorial, vamos descrever alguns algoritmos de visão computacional para a seg- mentação, indexação e recuperação de vídeo utilizando o OpenCV para que, ao final do tutorial, você

Thiago Teixeira Santos; Carlos Hitoshi Morimoto

59

Visitor face tracking system using OpenCV library  

Microsoft Academic Search

This paper presents the development of visitor face tracking system using OpenCV library. This system can be used for security purpose to record the visitor face. The user can identify who had been visiting him\\/her when away from the place. Visitors just need to appear in front of the web camera for at least 10 second, their image will be

Yong Kok Ching; A. S. Prabuwono; R. Sulaiman

2009-01-01

60

The Implementation of Lane Detective Based on OpenCV  

Microsoft Academic Search

In intelligent vehicle systems, lane detection is one of the most important parts. This paper presents a lane detection algorithm that based on Hough transform. Principle of the algorithm and the implementation base on OpenCV are discussed in detail. The algorithm was verified at the end of this paper.

Wu Ye; Shan Yuetian; Xu Yunhe; Wang Shu; Zhuang Yuchen

2010-01-01

61

Properties of NAC and CV[NAC] for Energy Models  

E-print Network

ESL-TR-93/05-03 Properties of CV [NAC] for Linear Energy Models David K. Ruch Sam Houston State University k Energy Systems Laboratory May 1993 1. Introduction and Notation The stability of the NAC estimate for the PRISM model has been shown em...

Ruch, D. K.

1993-01-01

62

Avaliação da cv. Cabernet Sauvignon para elaboração de vinho tinto  

Microsoft Academic Search

SUMMARY EVALUATION OF THE CV. CABERNET SAUVIGNON IN THE MANUFACTURE OF RED WINE. The Cabernet Sauvignon grape was developed in Bordeaux, France. It was introduced in Brazil in 1921, but its cultivation in the viticultural region of Serra Gaúcha only grew since 1980. It is used in the manufacture of aged or young red wines. Due to its importance, this

Luiz Antenor Rizzon; Alberto Miele

2002-01-01

63

C. Atsriku_CV1 Christian Atsriku (Ph.D)  

E-print Network

OF EXPERTISE: Drug metabolism Studies for small molecules: Metabolite profiling and identification for cold and quantification of post-translational modifications, protein enrichment and purification techniques Cell Cultures (1987-1991): University of Science and Technology, Kumasi Ghana #12;C. Atsriku_CV2 EMPLOYMENT

Lawson, Catherine L.

64

GpuCV: an opensource GPU-accelerated framework forimage processing and computer vision  

Microsoft Academic Search

This paper presents GpuCV, an open source multi-platform library for easily developing GPU-accelerated image processing and Computer Vision operators and applications. It is meant for computer vision scientist not familiar with GPU technologies. It is designed to be compatible with Intel's OpenCV library by offering GPU-accelerated operators that can be integrated into native OpenCV applications. The GpuCV framework transparently manages

Yannick Allusse; Patrick Horain; Ankit Agarwal; Cindula Saipriyadarshan

2008-01-01

65

An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection  

E-print Network

An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection for the Acceleration of OpenCV Object Detection Braiden Brousseau Master of Applied Science Graduate Department . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.4 OpenCV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.5 Multi

Rose, Jonathan

66

An Energy-Efficient, Fast FPGA Hardware Architecture for OpenCV-Compatible Object  

E-print Network

An Energy-Efficient, Fast FPGA Hardware Architecture for OpenCV-Compatible Object Detection Braiden source OpenCV computer vision software. The architecture is novel, compared to prior work in this area Computer Vision project, OpenCV [3]. We should note that the mobile context of this paper rests

Rose, Jonathan

67

Properties of a Cationic Peroxidase from Citrus jambhiri cv. Adalia  

Microsoft Academic Search

The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the\\u000a juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes\\u000a from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatography on diethylaminoethanol-cellulose,

Saleh A. Mohamed; Mohamed O. El-Badry; Ehab A. Drees; Afaf S. Fahmy

2008-01-01

68

A camera calibration technique based on OpenCV  

Microsoft Academic Search

Camera calibration is very improtant in 3D computer vision systems. In this paper, the camera model in OpenCV (open source computer vision library) is discussed, the non-linear distortion of the tangential and radial distortion aberration are considered. Based on these, we introduce the corner extraction of the Camera Calibration Toolbox for Matlab, and an arithmetic of camera calibration based on

Y. M. Wang; Y. Li; J. B. Zheng

2010-01-01

69

AVALIAÇÃO DA CV. MERLOT PARA ELABORAÇÃO DE VINHO TINTO 1  

Microsoft Academic Search

-1 de acidez titulável; e pH de 3,49. Sob o aspecto sensorial, o vinho Merlot apresenta cor vermelho-violáceo com boa intensidade. Gustativamente distingue-se pela maciez, equilíbrio e fineza. Palavras-chave: enologia; uva; Vitis vinifera; vinho; caracterização. SUMMARY EVALUATION OF CV. MERLOT TO MAKE RED WINE - Merlot is a traditional grape of the Saint Émillion wines, Bordeaux region - France. It

Luiz Antenor RIZZON; Alberto MIELE

2004-01-01

70

[On "the moxibustion shouldn't be applied at Shimen (CV 5) on female"].  

PubMed

The TCM theory "moxibustion shouldn't be applied at Shimen (CV 5) on female" is discussed in this article, which provides theoretical instruction on proper clinical application of Shimen (CV 5). Based on literature study, ancient classics concerning contraindication of "moxibustion shouldn't be applied at Shimen (CV 5) on female" were studied. TCM theory "moxibustion shouldn't be applied at Shimen (CV 5) on female" was explored profoundly through ancient record, physiological relations between Shimen (CV 5) and pregnancy, the relation between the anatomic structure of Shimen (CV 5) and the extra acupoint Jueyun Xue. And the result indicated that as an acupoint with contraindications, Shimen (CV 5) should be attached with great importance in its clinical practice on female patients. Reinforcing manipulation should be given instead of reducing. Moxibustion should be applied with cautiousness on the point, especially during pregnancy. PMID:23885628

Xuan, Da-Ke; Wu, Gui-Hua

2013-05-01

71

Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China.  

PubMed

Pigeon circovirus (PiCV) is one of four viruses in the family Circoviridae that affect young pigeons around the world. We collected 158 serum or tissue samples from six poultry farms in eastern China to investigate the prevalence and genetic characteristics of PiCV in Chinese pigeons. We tested for PiCV using a PCR assay and found that PiCV was present in 80.7 % (88/109) of diseased pigeons and 63.3 % (31/49) of healthy pigeons; overall, 75.3 % (119/158) of samples were PiCV positive. One PiCV-positive sample from each poultry farm was randomly chosen for amplification of the complete PiCV genome by inverse primer PCR (IP-PCR). The six genomic PiCV strains were designated as AHBZ (KJ704801), HBLF-E2 (KJ704802), JSJN (KJ704803), NJPK-21 (KJ704804), SDDZ (KJ704805) and SHWH-AB4 (KJ704806). We compared these new PiCV genomes to six publicly available PiCV genomes and found that the Rep and Cap genes had sequence identity ranging from 93.8 % to 100 % and 79.1 % to 100 %, respectively. In a phylogenetic analysis, PiCV and eight other members of the genus Circovirus were sister to chicken anemia virus (CAV), the only member of genus Gyrovirus. The results of this study provide evidence that PiCV is present in Chinese pigeons at a high rate and that PiCV is a viral lineage that is distinct from CAV. PMID:25348272

Zhang, Zhicheng; Dai, Wei; Wang, Shaohui; Dai, Dingzhen

2015-01-01

72

Odour-active compounds in papaya fruit cv. Red Maradol.  

PubMed

Application of solid-phase microextraction and simultaneous distillation-extraction combined with GC-FID, GC-MS, aroma extract dilution analysis, and odour activity value were used to analyse volatile compounds from papaya fruit cv. Red Maradol and to estimate the most odour-active compounds. The analyses led to the identification of 137 compounds; 118 of them were positively identified. Twenty-five odorants were considered as odour-active compounds and contribute to the typical papaya aroma, from which ethyl butanoate, benzyl isothiocyanate, 1-hexen-3-one, (E)-?-ionone, and methyl benzoate were the most odour-active compounds. PMID:24176322

Pino, Jorge A

2014-03-01

73

Magnetic CVs in the UCT CCD CV Survey  

E-print Network

An overview is given of all the magnetic CVs found in the UCT CCD CV Survey (Woudt & Warner 2001, 2002, 2003a). We have identified eight new candidate Intermediate Polars (IP), of which six are classical novae (RR Cha, DD Cir, AP Cru, V697 Sco, V373 Sct, and RX J1039.7-0507). The two other candidate IPs are Aqr1 (2236+0052) and RX J0944.5+0357. In addition, there are two probable Polars, namely V351 Pup (= Nova Puppis 1991) and FIRST J102347.6+003841.

Woudt, P A; Woudt, Patrick A.; Warner, Brian

2003-01-01

74

No nebular magnetization in the Allende CV carbonaceous chondrite  

NASA Astrophysics Data System (ADS)

Magnetic fields in the solar nebula may have played a central role in mass and angular momentum transport in the protosolar disk and facilitated the accretion of the first planetesimals. Thought to be key evidence for this hypothesis is the high unblocking-temperature, randomly oriented magnetization in chondrules in the Allende CV carbonaceous chondrite. However, it has recently been realized that most of the ferromagnetic minerals in Allende are products of secondary processes on the parent planetesimal. Here we reevaluate the pre-accretional magnetism hypothesis for Allende using new paleomagnetic analyses of chondrules including the first measurements of mutually oriented subsamples from within individual chondrules. We confirm that Allende chondrules carry a high-temperature component of magnetization that is randomly oriented among chondrules. However, we find that subsamples of individual chondrules are also non-unidirectionally magnetized. Therefore, the high-temperature magnetization in Allende chondrules is not a record of nebular magnetic fields and is instead best explained by remagnetization during metasomatism in a <8 ?T magnetic field. This low field intensity suggests that any core dynamo on the CV parent body decayed before the end of metasomatism, likely <40 My after the formation of calcium aluminum-rich inclusions (CAIs). Despite widespread practice, the magnetization in Allende should not be used to constrain magnetic fields in the protosolar nebula.

Fu, R. R.; Lima, E. A.; Weiss, B. P.

2014-10-01

75

The All-Sky Automated Survey for Supernovae CV Patrol  

NASA Astrophysics Data System (ADS)

Even in the modern era, only human eyes scan the entire optical sky for the violent, variable, and transient events that shape our universe. The "All Sky Automated Survey for Supernovae" (ASAS-SN or "Assassin") is changing this by monitoring the extra-galactic sky down to V~17 mag every 2-3 days using multiple telescopes, hosted by Las Cumbres Observatory Global Telescope Network, in the northern and southern hemispheres. By far the most common events observed by ASAS-SN are the Galactic transients. Since April 2013 ASAS-SN has identified over 180 new cataclysmic variable stars and announced over 260 new outbursts of known CVs. To make our data available to the CV community in 'real time', we have launched an automated 'CV Patrol' to monitor known CVs for outbursts as a useful tool for both professional and amateurs astronomers. It is a long term goal of ASAS-SN to make all our data public in real-time, and this patrol will serve as a framework for future ASAS-SN data releases.

Davis, Alexandra Bianca; Shappee, Benjamin John; Archer Shappee, Bartlett; ASAS-SN

2015-01-01

76

Fabric defect detection based on open source computer vision library OpenCV  

Microsoft Academic Search

A method for fabric defect detection based on OpenCV with rich computer vision and image processing algorithms and functions is presented. Firstly, OpenCV image processing functions implement fabric image preprocessing. We use morphological opening and closing operations to segment image because of their blur defects. Secondly, “seed filling” algorithm is applied to connect broke lines to keep defect edge smoothing.

Xiaojun Jia

2010-01-01

77

Research and improvement of face detection algorithm based on the OpenCV  

Microsoft Academic Search

This article describes functions and advantages of the OpenCV library, and explains the meaning, development status, applications, and difficulties of face detection technology, analysis the idea of Adaboost classifier algorithm, and achieves the detection procedures for face using OpenCV, and proposes the improved method based on the the original algorithm.

Min Zuo; Guangping Zeng; Xuyan Tu

2010-01-01

78

A Preliminary Investigation on Computer Vision for Telemedicine Systems Using OpenCV  

Microsoft Academic Search

OpenCV is typically, an open source vision library suitable for computer vision programs. In this paper, we present some of our preliminary investigation experiences of developing Computer Vision programs using OpenCV for robotic telemedicine cluster system, within the practice based ICTD subject within the undergraduate Software Engineering Program at the Faculty of Engineering, University of Technology Sydney (UTS). Firstly, it

Zenon Chaczko; LaReine A. Yeoh; Venkatesh Mahadevan

2010-01-01

79

Chondrule Magnetizations in the Allende CV Chondrite and Implications for the Dynamo of the CV Parent Body  

NASA Astrophysics Data System (ADS)

Many early-accreting planetesimals larger than several tens of km in diameter underwent extensive interior melting and differentiation. Advection in the molten metallic cores of these planetesimals may have generated magnetic dynamos. Remanent magnetization preserved in meteorites can reveal the past presence of core dynamo fields and therefore a metallic core on their parent bodies. Furthermore, the meteoritic magnetic record can constrain the duration of the dynamo, providing insight into the thermal evolution of the parent planetesimal. Carporzen et al. (2011) argued that bulk samples of the Allende CV carbonaceous chondrite carry a unidirectional partial thermoremanent magnetization (pTRM) blocked up to ~290C. They interpreted this magnetization as recording a magnetic core dynamo on the CV parent body. However, the previous study provided no constraints on the duration of the dynamo and did not characterize the magnetic recording in each component of the Allende meteorite. We conducted paleomagnetic experiments on 23 mutually oriented individual Allende chondrules and matrix samples. We also studied mutually oriented subsamples of 9 of these chondrules. We found that Allende chondrules can be divided into two distinct classes based on their natural remanent magnetization (NRM). Class A chondrules carry a strong low temperature overprint parallel to that of bulk Allende and matrix material that also unblocks at ~290C. Class B chondrules do not carry this low temperature overprint and exhibit randomly oriented NRMs. Electron microprobe analysis and thermal demagnetization of saturation remanence showed that magnetic phases in both Class A and Class B chondrules are likely products of parent body metasomatism. We infer that the random magnetization of Class B chondrules as well as the magnetization blocked above 290C in Class A chondrules and matrix material is a chemical remanent magnetization that resulted in randomly oriented remanence at the sub-millimeter scale. Allende chondrules therefore do not carry pre-accretional magnetization. The presence of a unidirectional pTRM overprint in Class A chondrules and matrix material but not in Class B chondrules requires a process that removed the pTRM overprint in Class B chondrules after its acquisition. We infer that, while all Allende components experienced metasomatic recrystallization of their magnetic phases, Class A chondrules completed this process before the pTRM event while Class B chondrules underwent metasomatism after the pTRM event. Furthermore, the random magnetization directions of Class B chondrules may suggest that a stable dynamo field no longer existed during the period of aqueous alteration, thereby constraining the lifetime of the CV parent body dynamo to less than several tens of My.

Weiss, B. P.; Fu, R. R.

2012-12-01

80

Short-term cold stress in two cultivars of Digitaria eriantha : effects on stress-related hormones and antioxidant defense system  

Microsoft Academic Search

The two cultivars of Digitaria eriantha: cv. Sudafricana (a cold-sensitive cultivar) and cv. Mejorada INTA (a cold-resistant cultivar) were exposed to low temperature\\u000a and compared in terms of the involvement of abscisic acid (ABA) and catabolites, jasmonates, and antioxidant defense in cold\\u000a tolerance. Cold stress caused a greater ABA increase in cv. Mejorada INTA than in cv. Sudafricana. In both

M. Garbero; H. Pedranzani; F. Zirulnik; A. Molina; M. V. Pérez-Chaca; A. Vigliocco; G. Abdala

2011-01-01

81

Solution NMR Structure of Hypothetical Protein CV_2116 Encoded by a Viral Prophage Element in Chromobacterium violaceum  

SciTech Connect

CV{_}2116 from Chromobacterium violaceum is a small hypothetical protein of 82 amino acids. A PSI-BLAST search using the CV{_}2116 protein sequence as a query identified only two hits, both with amino acid sequence identities of less than 40%. After the CV{_}2116 gene was cloned into the p15TvLic expression plasmid and transformed into E. coli, the desired CV{_}2116 protein was expressed and purified. A high quality solution structure of CV{_}2116 was determined by NMR spectroscopy. The tertiary structure of CV{_}2116 adopts a novel alpha + beta fold containing two anti-parallel beta sheets and one alpha helix in the C-terminal end. CV{_}2116 does not belong to any known protein sequence families and no similar structures exist in the protein data bank. To date, no function of CV{_}2116 can be derived from either sequence or structural similarity searches.

Yang, Yunhuang; Ramelot, Theresa A.; Cort, John R.; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl H.; Kennedy, Michael A.

2012-06-14

82

Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual Machine  

E-print Network

Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual jblynch@ucsd.edu Abstract The OpenCV framework is an open source project released under the BSD license will focus on the OpenCV framework, an open source project that furnishes developers with a series

California at Santa Barbara, University of

83

Candesartan (CV11974) dissociates slowly from the angiotensin AT 1 receptor  

Microsoft Academic Search

The mechanisms of the insurmountable antagonism of 2-ethoxy-1-[[2?-(1 H-tetrazol-5-yl)biphenyl-4-yl]methyl]-1 H-benzimidazole-7-carboxylic acid, candesartan (CV-11974), an angiotensin AT1 receptor antagonist, on angiotensin II-induced rabbit aortic contraction were examined in contraction and binding studies. Preincubation of the rabbit aorta with CV-11974 (0.1 nM) for 30 min reduced the maximal contractile response to angiotensin II by approximately 50%. This insurmountable antagonism of CV-11974 was

Mami Ojima; Yoshiyuki Inada; Yumiko Shibouta; Takeo Wada; Tsukasa Sanada; Keiji Kubo; Kohei Nishikawa

1997-01-01

84

Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) Somaclones  

NASA Technical Reports Server (NTRS)

The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.

Campbell, W. F.; Carman, J. G.; Hashim, Z. N.

1990-01-01

85

Infrared small target detection technology based on OpenCV  

NASA Astrophysics Data System (ADS)

Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

Liu, Lei; Huang, Zhijian

2013-09-01

86

Infrared small target detection technology based on OpenCV  

NASA Astrophysics Data System (ADS)

Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

Liu, Lei; Huang, Zhijian

2013-05-01

87

[Characteristics of Papaver somniferum L. cv. ikkanshu cultivated in Izu].  

PubMed

The seeds of Papaver somniferum L. cv. Ikkanshu were sown in November (Autumn sowing: AS) and March (Spring sowing: SS) in a field at Izu Experimental Station for Medicinal Plants of National Institute of Health Sciencs, and both AS and SS plants were cultivated to investigate their growth, opium yield and alkaloid content in the opium. Growing periods from the sowing to the opium harvest were approximately six months for AS plants and three months for SS plants. Sizes of plants and capsules in AS were bigger than those in SS, reflecting their growth period. Opium yields per an are in AS and SS were 212.09 g and 142.03 g, respectively. The opium was able to be collected four times in the AS plants though the SS plants ceased to exude opium after the second incision. Therefore higher yield of opium in AS plants seems to be attributed to an amount of opium in the third and fourth incision. Average morphine content in the total opium was 15.61% in AS plants and 15.04% in SS plants, and the estimated amounts of morphine per an are in AS and SS plants were 33.16 g and 21.38 g, respectively. PMID:11534131

Iida, O; Sekine, T; Inoue, O; Yoshimatsu, K; Shimomura, K

2000-01-01

88

Limited geographic distribution of the novel cyclovirus CyCV-VN.  

PubMed

A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600?CSF specimens from patients with suspected CNS infections in northern Vietnam, Cambodia, Nepal and The Netherlands were screened for the presence of CyCV-VN but all were negative. Sequence comparison and phylogenetic analysis between CyCV-VN and another novel cyclovirus recently identified in CSF from Malawian patients indicated that these represent distinct cycloviral species, albeit phylogenetically closely related. The data suggest that CyCV-VN has a limited geographic distribution within southern and central Vietnam. Further research is needed to determine the global distribution and diversity of cycloviruses and importantly their possible association with human disease. PMID:24495921

Le, Van Tan; de Jong, Menno D; Nguyen, Van Kinh; Nguyen, Vu Trung; Taylor, Walter; Wertheim, Heiman F L; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Nguyen, Hanh Uyen; Giri, Abhishek; Nguyen, Thi Thuy Chinh Bkrong; Ho, Dang Trung Nghia; Farrar, Jeremy; Bryant, Juliet E; Tran, Tinh Hien; Nguyen, Van Vinh Chau; van Doorn, H Rogier

2014-01-01

89

Impact-Induced Compaction in CV Chondrites: Exploring a Hidden Record with EBSD  

NASA Astrophysics Data System (ADS)

We present impact-induced textural features found within the previously S1-classified CV, Allende. Features include high amounts of intragrain strain within the fine matrix grains, and moderate strain towards chondrule edges.

Forman, L. V.; Bland, P. A.; Timms, N. E.; Benedix, G. K.

2014-09-01

90

Cathodoluminescence Microcharacterization of Forsterite from Kaba CV3 Chondrite: Implication for Mineralogy and Petrology of IDPs  

Microsoft Academic Search

Cathodoluminescence studies of forsterite from the fine fragment sample of Kaba indicate that mineral fragments from low altered and low thermally metamorphic CV3 chondrite might be potentially useful reference material for the IDPs.

Sz. Bérczi; A. Gucsik; T. Okumura; K. Ninagawa; H. Nishido; Á. Kereszturi; Sz. Nagy; H. Hargitai

2008-01-01

91

Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV).  

PubMed

Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with IkappaB-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy. PMID:18353418

Shi, Min; Chen, Ya-Feng; Huang, Fang; Liu, Peng-Cheng; Zhou, Xue-Ping; Chen, Xue-Xin

2008-06-01

92

Phyllosilicates in the Mokoia CV carbonaceous chondrite - Evidence for aqueous alteration in an oxidizing environment  

NASA Technical Reports Server (NTRS)

Most CV chondrites contain little if any phyllosilicate mineralization. A petrographic and transmission electron microscopy study of the Mokoia CV carbonaceous chondrite shows that the matrix, chondrules, aggregates, and inclusions all contain considerable amounts of phyllosilicates. The mineralogy and occurrence of phyllosilicates in Mokoia differ from those in the CI and CM chondrites. The differences suggests that aqueous alteration of the three meteorite groups probably occurred under a variety of conditions.

Tomeoka, Kazushige; Buseck, Peter R.

1990-01-01

93

Full split C-V method for parameter extraction in ultra thin BOX FDSOI MOS devices  

NASA Astrophysics Data System (ADS)

The feasibility of full split C-V method in ultra-thin body and BOX (UTBB) FDSOI devices is demonstrated, emphasizing the usefulness of gate-to-bulk capacitance. The split C-V measurements carried out on both gate-to-channel and gate-to-bulk mode are shown to be consistent with TCAD simulation. This enabled us to propose an improved parameter extraction methodology for the whole vertical FDSOI stack from gate to substrate using back biasing effect.

Shin, Minju; Shi, Ming; Mouis, Mireille; Cros, Antoine; Josse, Emmanuel; Kim, Gyu-Tae; Ghibaudo, Gérard

2014-09-01

94

Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa.  

PubMed

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution. PMID:25518947

Garigliany, Mutien-Marie; Hagen, Ralf Matthias; Frickmann, Hagen; May, Jürgen; Schwarz, Norbert Georg; Perse, Amanda; Jöst, Hanna; Börstler, Jessica; Shahhosseini, Nariman; Desmecht, Daniel; Mbunkah, Herbert Afegenwi; Daniel, Achukwi Mbunkah; Kingsley, Manchang Tanyi; Campos, Renata de Mendonca; de Paula, Vanessa Salete; Randriamampionona, Njary; Poppert, Sven; Tannich, Egbert; Rakotozandrindrainy, Raphael; Cadar, Daniel; Schmidt-Chanasit, Jonas

2014-01-01

95

The comparison of CPU time consumption for image processing algorithm in Matlab and OpenCV  

Microsoft Academic Search

In order to fill gap of growing demand for high efficient image and video processing, open source computer vision library (OpenCv) is way to deals with this task. Hence, this paper is about basic algorithm for image processing and their CPU time consumption in Matlab comparing with OpenCv. Algorithms are tested on images with resolution 3264×2448, 1920×1080, 1024×768 and 220×260.

Slavomir Matuska; Robert Hudec; Miroslav Benco

2012-01-01

96

GpuCV: A GPU-Accelerated Framework for Image Processing and Computer Vision  

Microsoft Academic Search

This paper presents briefly the state of the art of accelerating image processing with graphics hardware (GPU) and discusses\\u000a some of its caveats. Then it describes GpuCV, an open source multi-platform library for GPU-accelerated image processing and\\u000a Computer Vision operators and applications. It is meant for computer vision scientist not familiar with GPU technologies.\\u000a GpuCV is designed to be compatible

Yannick Allusse; Patrick Horain; Ankit Agarwal; Cindula Saipriyadarshan

2008-01-01

97

Multi-camera calibration based on openCV and multi-view registration  

Microsoft Academic Search

For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the

Xiao-Ming Deng; Xiong Wan; Zhi-Min Zhang; Bi-Yan Leng; Ning-Ning Lou; Shuai He

2010-01-01

98

Proteins responding to drought and high-temperature stress in Populus × euramericana cv. ‘74\\/76’  

Microsoft Academic Search

Proteomic analysis provides a powerful method of studying plant responses to stress at the protein level. In order to study\\u000a stress-responsive molecular mechanisms for Populus × euramericana cv. ‘74\\/76’, one of the most important forest plantation tree species in subtropical and temperate regions, we analyzed the\\u000a response of 2-year-old cuttings of P. × euramericana cv. ‘74\\/76’ to drought and high

Caiyun He; Jianguo Zhang; Aiguo Duan; Shuxing Zheng; Honggang Sun; Lihua Fu

2008-01-01

99

Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV)  

SciTech Connect

Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with I{kappa}B-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy.

Shi Min; Chen Yafeng; Huang Fang; Liu Pengcheng [Institute of Insect Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029 (China); Zhou Xueping [Institute of Biotechnology, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029 (China); Chen Xuexin [Institute of Insect Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029 (China)], E-mail: xxchen@zju.edu.cn

2008-06-05

100

Properties of a cationic peroxidase from Citrus jambhiri cv. Adalia.  

PubMed

The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatography on diethylaminoethanol-cellulose, carboxymethyl-cellulose, and Sephacryl S-200 columns. Cationic peroxidase POII is proved to be pure, and its molecular weight was 56 kDa. A study of substrate specificity identified the physiological role of POII, which catalyzed the oxidation of some phenolic substrates in the order of o-phenylenediamine > guaiacol > o-dianisidine > pyrogallol > catechol. The kinetic parameters (K (m), V (max), and V (max)/K (m)) of POII for hydrolysis toward H2O2 and electron donor substrates were studied. The enzyme had pH and temperature optima at 5.5 and 40 degrees C, respectively. POII was stable at 10-40 degrees C and unstable above 50 degrees C. The thermal inactivation profile of POII is biphasic and characterized by a rapid decline in activity on exposure to heat. The most of POII activity (70-80%) was lost at 50, 60, and 70 degrees C after 15, 10, and 5 min of incubation, respectively. Most of the examined metal ions had a very slight effect on POII except of Li+, Zn2+, and Hg2+, which had partial inhibitory effects. In the present study, the instability of peroxidase above 50 degrees C makes the high temperature short time treatment very efficient for the inactivation of peel peroxidase contaminated in orange juice to avoid the formation of off-flavors. PMID:18633734

Mohamed, Saleh A; El-Badry, Mohamed O; Drees, Ehab A; Fahmy, Afaf S

2008-08-01

101

CV Med Fellow Arrhythmia Service Rotation The goal of the Arrhythmia Service rotation is provide the CV Med Fellow with a  

E-print Network

the CV Med Fellow with a comprehensive experience in the diagnosis and treatment of cardiac arrhythmias ECG stress tests. · Describe to a physician or patient the basic elements, success rate, and risks and Cardiac Electrophysiology Laboratory Sung Chun, MD, Director, Pacemaker and ICD Service Karen Friday, MD

Ford, James

102

Circulating HFMD-Associated Coxsackievirus A16 Is Genetically and Phenotypically Distinct from the Prototype CV-A16  

PubMed Central

Human enteroviruses (HEV) have been linked to hand, foot, and mouth disease (HFMD) in the Pacific and Southeast Asia for decades. Many cases of HFMD have been attributed to coxsackievirus A16 (CV-A16, CA16), based on only partial viral genome determination. Viral phenotypes are also poorly defined. Herein, we have genetically and phenotypically characterized multiple circulating CV-A16 viruses from HFMD patients and determined multiple full-length sequences of these circulating viruses. We discovered that the circulating CV-A16 viruses from HFMD patients are genetically distinct from the proto-type CV-A16 G10. We have also isolated circulating CV-A16 viruses from hospitalized HFMD patients and compared their virological differences. Interestingly, circulating CV-A16 viruses are more pathogenic in a neonatal mouse model than is CV-A16 G10. Thus, we have found circulating recombinant forms of CV-A16 (CRF CV-A16) that are related to, but different from, the prototype CV-A16 G10 that have distinct biological phenotypes. PMID:24736564

Li, Jingliang; Ren, Sangsang; Wei, Zhenhong; Bao, Wanguo; Hu, Xiaoming; Zhao, Ke; Zhang, Wenyan; Zhou, Yulai; Sun, Fei; Markham, Richard; Yu, Xiao-Fang

2014-01-01

103

Inhibition of lipid peroxidation by a novel compound (CV-2619) in brain mitochondria and mode of action of the inhibition.  

PubMed

Lipid peroxidation in rat brain mitochondria was induced by NADH in the presence of ADP and FeCl3. CV-2619 inhibited the lipid peroxidation in a concentration-dependent manner; the concentration giving 50% inhibition (IC50) was 84 microM. In addition, the inhibitory effect of CV-2619 was strongly enhanced by adding substrates of mitochondrial respiration; when succinate, glutamate, or succinate plus glutamate was added, the IC50 of CV-2619 was changed to 1.1, 10, or 0.5 microM, respectively. Metabolites of CV-2619 also inhibited the lipid peroxidation. The inhibitory effect of CV-2619 on mitochondrial lipid peroxidation disappeared when TTFA, an inhibitor of complex II in mitochondrial respiratory chain, was added. The results indicate that in mitochondria CV-2619 is changed to its reduced form which inhibits lipid peroxidation. PMID:6517932

Suno, M; Nagaoka, A

1984-12-28

104

Fusion PCR-targeted tylCV gene deletion of Streptomyces fradiae for producing desmycosin, the direct precursor of tilmicosin  

Microsoft Academic Search

Tilmicosin is a semisynthetic macrolide antibiotic derived from tylosin. The disruption of tylCV gene from tylosin producer—Streptomyces fradiae would result in the accumulation of desmycosin, the direct precursor of tilmicosin. TylCV gene disruption cassette was constructed by fusion PCR. The tylCV replacement strain of S. fradiae was obtained through intergeneric conjugation between S. fradiae and E. coli. The antibiotic resistance

Yong Min; Heping Lv; Yinghua Zheng

2007-01-01

105

Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.  

PubMed

Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater. PMID:25700813

Matsushima, Kazuki; Kaneda, Hirokazu; Harada, Kazuo; Matsuura, Hideyuki; Hirata, Kazumasa

2015-05-01

106

Segmentation of kidney using C-V model and anatomy priors  

NASA Astrophysics Data System (ADS)

This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

2007-12-01

107

Discovery of a Stable Dimeric Mutant of Cyanovirin-N (CV-N) from a T7 Phage-Displayed CV-N Mutant Library  

Microsoft Academic Search

Mutant proteins with altered properties can be useful probes for investigating structure, ligand binding sites, mechanisms of action, and physicochemical attributes of the corresponding wild-type proteins of interest. In this report, we illuminate properties of mutants of the potent HIV-inactivating protein, cyanovirin-N (CV-N), selected by construction of a mutant library by error-prone polymerase chain reaction and affinity-based screening using T7

Zhaozhong Han; Changyun Xiong; Toshiyuki Mori; Michael R. Boyd

2002-01-01

108

Evaluation of antitranspirants on creeping bentgrass (Agrostis palustris Huds., cv. 'Penncros') and bermudagrass (Cynodon dactylon (L.) Pers. X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway')  

E-print Network

. X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway' (Tifway ber- mudagrass). The turfs were clipped to a 2. 5 cm mowing height during the study. Testing was done in a controlled environment chamber where conditions were chosen to give the maximum... evapotranspiration rate possible in the chamber for well-watered turf. Air temperature, point carbondioxide level, photosynthetic photon flux density, and wind speed were controlled throughout the study. Experiments lasted between 48 and 72 hours. Hater loss from...

Stahnke, Gwen Kristine

1981-01-01

109

Learning OpenCV---Computer Vision with the OpenCV Library (Bradski, G.R. et al.; 2008)[On the Shelf  

Microsoft Academic Search

This is an introductory textbook for teachers, students, professionals, and hobbyists who want to learn the basics of computer vision. The book is completely based around the OpenCV library, an open source project that started in 1999 by the computer-vision community. The authors of the text are among the principal contributors to this real-time library that has developed in C\\/C++

Alex Zelinsky

2009-01-01

110

The chemical composition of suberin in apoplastic barriers affects radial hydraulic conductivity differently in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix)  

Microsoft Academic Search

Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degrada- tion (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were ana- lysed quantitatively by gas chromatography and mass spectrometry. In corn, suberin was determined for isolated endodermal (ECW) and rhizo-hypodermal (RHCW) cell

Lukas Schreiber; Rochus Franke; Klaus-Dieter Hartmann; Kosala Ranathunge; Ernst Steudle

2005-01-01

111

Martin O. Saar CV, Page 1 of 20 10/11/2010 Note: Publications are listed toward the end.  

E-print Network

Martin O. Saar CV, Page 1 of 20 10/11/2010 Note: Publications are listed toward the end. CURRICULUM VITAE Martin O. Saar E-mail: saar@umn.edu Department of Geology and Geophysics Office: +1 612 of America (GSA) 1997-date American Geophysical Union (AGU) #12;Martin O. Saar CV, Page 2 of 20 10

Saar, Martin O.

112

Kenmerk: CvB 2008/879 Afspraken rond invoering UFO  

E-print Network

Kenmerk: CvB 2008/879 Afspraken rond invoering UFO hulpinstrument voor secretaressefuncties Rond de invoering van een UFO hulpinstrument voor de indeling van secretaressefuncties hebben het College van vergemakkelijken. Op de beleids- en uitvoeringsafspraken van UFO heeft dit geen effect; het is een additioneel

Franssen, Michael

113

EST-SSR MARKERS FROM FRAGARIA VESCA L. CV. YELLOW WONDER  

Technology Transfer Automated Retrieval System (TEKTRAN)

Fourteen microsatellite primer pairs were developed from a cDNA library of Fragaria vesca cv. Yellow Wonder. Transferability to 13 species of Fragaria ranged from 71% in diploid species F. gracilis Losinsk., F. iinumae Makino, F. nilgerrensis Schltdl. ex J. Gay and F. nipponica Makino to 100% in the...

114

Outburst of CV ROTSE3 J031031.4+431115.0  

NASA Astrophysics Data System (ADS)

Further to ATel#1272, we report observations of an outburst of the U Geminorum-type CV ROTSE3 J031031.4+431115.0 in unfiltered CCD images taken by the 0.45 m ROTSE-IIIb telescope at McDonald Observatory, Texas.

Dhungana, G.; Ferrante, F. V.; Staten, R.; Kehoe, R.

2015-02-01

115

Thermal degradation of anthocyanins from purple potato (Cv. Purple Majesty) and their impact on antioxidant capacity  

Technology Transfer Automated Retrieval System (TEKTRAN)

Degradation parameters of purified anthocyanins from purple-fleshed potato (Purple Majesty cv.) heated at high temperatures (100 - 150 °C) was determined. Purified anthocyanins, prepared by removing salts, sugars and colorless non-anthocyanin phenolics from the crude extract, were quantified using H...

116

Alian Wang: CV (December 2013) Curriculum Vita of Prof. Alian Wang  

E-print Network

-ASTID (Astrobiology Science and Technology Instrument Development) projects, for developing WIR and BUF sensors. 2004Alian Wang: CV (December 2013) 1 Curriculum Vita of Prof. Alian Wang Education: Ph.D. (1987 (Astrobiology Science and Technology for Exploring Planets) project, "life in Atacama". 2011 ­ 2012, PI, a NASA

117

Simple Cp/Cv Resonance Apparatus Suitable for the Physics Teaching Laboratory.  

ERIC Educational Resources Information Center

Describes a resonance apparatus for the measurement of Cp/Cv for different gases. In the apparatus a magnetically supported piston in a vertical cylindrical glass tube containing the gas is forced into oscillation by means of a standard audio signal generator. (Author/GA)

Smith, D. G.

1979-01-01

118

Indirect measurements of Brachiaria brizantha cv. Marandu fermentable cell wall sugars for second generation biofuels production.  

Technology Transfer Automated Retrieval System (TEKTRAN)

Results of a study conducted to evaluate the possibility of using IVDMD values of B. brizantha cv. Marandu to predict cell wall sugars that would be available in a biorefinery for ethanol production are reported. The study was conducted based on the similarity between rumen enzymes and those used i...

119

Agrobacterium-mediated transformation of Easter lily (Lilium longiflorum cv. Nellie White)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Conditions were optimized for transient transformation of Lilium longiflorum cv. Nellie White using Agrobacterium tumefaciens. Bulb scale and basal meristem explants were inoculated with A. tumefaciens strain AGL1 containing the binary vector pCAMBIA 2301 which has the uidA gene that codes for ß-gl...

120

The influence of nitrate nutrition on H + efflux by young rape plants ( Brassica napus cv. emerald)  

Microsoft Academic Search

Changes in pH around the roots of young rape plants (Brassica napus cv. emerald) were studied using a nutrient film technique that allowed part or whole of the root system to be subjected to specific nutrient treatments. The rapidity and direction of pH change was assessed by imbedding absorbing roots in a thin film of agar containing bromocresol purple. When

H. Moorby; P. H. Nye; R. E. White

1985-01-01

121

Antibiotic targeting of the bacterial secretory pathway Smitha Rao C.V. a  

E-print Network

Review Antibiotic targeting of the bacterial secretory pathway Smitha Rao C.V. a , Evelien De xxxx Keywords: Antibiotic target Protein secretion inhibitor Signal peptidase SecA Antibiotic resistance Finding new, effective antibiotics is a challenging research area driven by novel approaches

Economou, Tassos

122

Petrological Investigations of CAIs from Efremovka and NWA 3118 CV3 Chondrites  

NASA Astrophysics Data System (ADS)

Several new big CAIs were extracted from the Efremovka and NWA 3118 CV3 chondrites to analyze petrology, chemistry and isotopic compositions. Here we report preliminary results on mineralogy, petrology and bulk chemistry of two CAIs, of Type B1 and of Type A.

Ivanova, M. A.; Lorenz, C. A.; Korochantseva, E. V.; MacPherson, G. J.

2010-03-01

123

Pre-Accretionary Distribution of Ca and Al Between Matrix and Chondrules in CV Chondrites  

NASA Astrophysics Data System (ADS)

Ca/Al-ratios in Y-86751 (CV) chondrules are super- and in matrix sub-chondritic. The opposite is true for Allende and Efremovka. Incorporation of spinel in Allende and Efremovka chondrule precursors in a nebular setting can explain this observation.

Hezel, D. C.; Palme, H.

2007-03-01

124

High productivity multiple DUT CV test for MEMS microphone wafer with automatic correction  

Microsoft Academic Search

Productivity in MEMS wafer process is getting more and more important as mass production on 200 mm wafer is increasing. Multiple DUT parallel CV test is a high productive way for MEMS Microphone wafer test process, however, in case of the one of two electrodes is connected to the wafer substrate with some contact resistance, interference among DUTs has an

S. Inuzuka

2010-01-01

125

Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1)  

Technology Transfer Automated Retrieval System (TEKTRAN)

The distribution of a potential biological agent, the newly discovered leafhopper-infecting virus, HoCV-1 was examined across the USA. Few biological agents exist for use against leafhopper pests. We examined and compared leafhopper salivary gland and midgut tissues using transmission electron micro...

126

CV D. Antoine, updated August 2013 David ANTOINE, born 21 January 1965, Saint-Brieuc, France  

E-print Network

CV D. Antoine, updated August 2013 1 David ANTOINE, born 21 January 1965, Saint-Brieuc, France, 06230 Villefranche sur mer, France Tel +33 4 93 76 37 23 Fax +33 4 93 76 37 39 antoine@obs-vlfr.fr http vitae Education 2005 D. Sc. in oceanography Université Pierre et Marie Curie / Paris 6 (UPMC), France

Antoine, David

127

Comportement du lupin blanc, Lupinus albus L, cv Lublanc, en sols calcaires. Seuils de tolrance  

E-print Network

Agronomie Comportement du lupin blanc, Lupinus albus L, cv Lublanc, en sols calcaires. Seuils de essai de comportement du lupin blanc, cultivar Lublanc. Ce comportement a également été observé, dans un. Les indices de pouvoir chorosant se sont révélés de moins bons estimateurs du comportement du lupin

Paris-Sud XI, Université de

128

THE VALUE OF LUPINUS ALBUS L. CV. AU HOMER AS A WINTER COVER CROP FOR COTTON  

Technology Transfer Automated Retrieval System (TEKTRAN)

Successful cotton (Gossypium hirsutum L.) management in the southeastern USA with conservation tillage requires the utilization of winter cover crops to increase organic matter in the top 5 cm of the soil. The objective of our research was to test the newly-developed bitter white lupin cv. `AU Home...

129

Optimized growth and plant regeneration for callus of Lilium longiflorum cv. Nellie White  

Technology Transfer Automated Retrieval System (TEKTRAN)

The rates of growth and regeneration were compared for compact callus, friable callus, and suspension cells of Lilium longiflorum cv. Nellie White to determine the optimal culture conditions. The fresh weight was higher for compact callus induced from bulb scales cultured on Murashige and Skoog’s m...

130

Tolerance of mycorrhized banana ( Musa sp. cv. Pacovan) plantlets to saline stress  

Microsoft Academic Search

Arbuscular mycorrhizal fungi (AMF) are known to increase plant tolerance to abiotic stress, in particular soil salinity. Isolates of Acaulospora scrobiculata Trappe, Glomus clarum Nicolson & Schenck and Glomus etunicatum Becker & Gerdemann were investigated under glasshouse conditions. Inoculated banana plants (Musa sp. cv. Pacovan) showed higher nutrient contents and growth rates than the controls. G. clarum was particularly efficient

Adriana Mayumi Yano-Melo; Orivaldo José Saggin; Leonor Costa Maia

2003-01-01

131

Teaching image processing and pattern recognition with the Intel OpenCV library  

Microsoft Academic Search

In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image

Adam Kozlowski; Aleksandra Królak

2009-01-01

132

Registered Localization Method-based on OpenCV for Augmented Reality  

Microsoft Academic Search

Augmented Reality is a kind of technique combining a virtual world with a real environment. This paper tells that OpenCV is applied to the realization of Registration Technique which is key to Augmented Reality. It studies the implementation of the camera calibration, the extraction of characteristic target and its recognition. This paper improved the efficiency of the exploitation and laid

Jun Dai; Li-fen Zhang

2011-01-01

133

Recognition assisted dynamic surveillance system based on OSGi and OpenCV  

Microsoft Academic Search

Thanks to the Internet development, a surveillance user can monitor the distant events via a browser on a computer. These events can be even viewed on a mobile phone with the assistance from modern mobile networks. Besides, two well-known platforms - OSGi and OpenCV are widely used for application construction. OSGi is constructed to provide a service platform with high

Jenq-Shiou Leu; Wei-Hsiang Lin; Min-Chieh Yu; Hung-Jie Tzeng

2011-01-01

134

OpenCV compatible real time processor for background foreground identification  

Microsoft Academic Search

The background identification methods are used in many fields like video surveillance and traffic monitoring. In this paper we propose a hardware implementation of the Gaussian Mixture Model algorithm able to perform background identification on HD images. The proposed circuit is based on the OpenCV implementation, particularly suited to improve the initial background learning phase. Bit-width has been optimized in

M. Genovese; E. Napoli; N. Petra

2010-01-01

135

Automatic tracking of red blood cells in micro channels using OpenCV  

NASA Astrophysics Data System (ADS)

The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

2013-10-01

136

Identification of HoCV-1 virus in Texas Glassy-Winged Sharpshooter Populations  

Technology Transfer Automated Retrieval System (TEKTRAN)

A new strain of leafhopper virus was discovered in leafhoppers which are vectors of Pierce’s disease of grapes. The original leafhopper infecting virus, HoCV-1, was isolated from the glassy-winged sharpshooter, Homalodisca vitripennis, from samples collected in California and Florida. Using the orig...

137

Pro-inflammatory effect in mice of CvL, a lectin from the marine sponge Cliona varians.  

PubMed

CvL, a lectin from the marine sponge Cliona varians agglutinated type A papainized erythrocytes and was strongly inhibited by d-galactose and sucrose. Models of leukocyte migration in vivo were used to study the inflammatory activity of CvL through of mouse paw oedema and peritonitis. Effect of CvL on peritoneal macrophage activation was analysed. Effects of corticoids and NSAIDS drugs were also evaluated on peritonitis stimulated by CvL. Results showed that mouse hind-paw oedema induced by subplantar injections of CvL was dose dependent until 50 microg/cavity. This CvL dose when administered into mouse peritoneal cavities induced maxima cell migration (9283 cells/microL) at 24 h after injection. This effect was preferentially inhibited by incubation of CvL with the carbohydrates d-galactose followed by sucrose. Pre-treatment of mice with 3% thioglycolate increases the peritoneal macrophage population 2.3 times, and enhanced the neutrophil migration after 24 h CvL injection (75.8%, p<0.001) and no significant effect was observed in the presence of fMLP. Finally, pre-treatment of mice with dexamethasone (cytokine antagonist) decreased (65.6%, p<0.001), diclofenac (non-selective NSAID) decreased (34.5%, p<0.001) and Celecoxib (selective NSAID) had no effect on leukocyte migration after submission at peritonitis stimulated by CvL, respectively. Summarizing, data suggest that CvL shows pro-inflammatory activity, inducing neutrophil migration probably by pathway on resident macrophage activation and on chemotaxis mediated by cytokines. PMID:17933589

Queiroz, Alexandre F S; Moura, Raniere M; Ribeiro, Jannison K C; Lyra, Ibson L; Cunha, Dayse C S; Santos, Elizeu A; de-Sales, Maurício P

2008-03-01

138

Modal mineralogy of CV3 chondrites by X-ray diffraction (PSD-XRD)  

NASA Astrophysics Data System (ADS)

Using position sensitive detector X-ray diffraction (PSD-XRD) we determine a complete modal mineralogy for all phases present in abundances greater than 1 wt% in Vigarano, Efremovka, Mokoia, Grosnaja, Kaba and Allende. Reduced CV3 samples are comprised of (vol%): olivine (83-85%); enstatite (6.5-8.1%); anorthite (1.1-1.2%); magnetite (1.4-1.8%); sulphide (2.4-5.1%); Fe, Ni metal (2-2.2%). The oxidized samples are comprised of: olivine (76.3-83.9%); enstatite (4.8-7.8%); anorthite (1.1-1.7%); magnetite (0.3-6.1%); sulphide (2.9-8.1%); Fe, Ni metal (0.2-1.1%); Fe-oxide (0-2.7%) and phyllosilicate (1.9-4.2%). When our modal data is used to calculate a bulk chemistry that is compared to literature data a near 1:1 correlation is observed. PSD-XRD data indicates that olivine compositions may span almost the entire Fe-Mg solid solution series in all CV samples and that these contain a component (4-13%) of fine-grained olivine that is more Fe-rich (>Fa 60) than is typically reported. Modal mineralogy shows that there are mineralogic differences between CV3 samples classified as oxidized and reduced but that these sub-classes are most clearly distinguished by the relative abundance of metal and Ni content of sulphide, rather than abundance of magnetite. The most significant difference in modal mineralogy observed is the relative absence of phyllosilicate in reduced CV that essentially escaped aqueous alteration. Fayalite, ferrous olivine and magnetite are typically considered secondary alteration products. The abundances of these minerals overlap in oxidized and reduced samples and correlate positively supporting common conditions of formation in a relatively oxidizing environment. The abundances of fayalite, ferrous olivine and magnetite show no relationship to petrographic type and if these abundances were used as a proxy for alteration, Allende would be the least altered CV - contrary to all previous data. The implication is that thermal metamorphism on the parent body was de-coupled from formation of Fe-rich secondary minerals. Low temperature fluid-assisted metamorphism can also not easily explain the origin of fayalite, ferrous olivine and magnetite, since the reduced CVs appear to be largely unaffected by this process. Parent body models require an anhydrous low-temperature mechanism of secondary alteration. The alternative is that these phases formed prior to accretion of the final CV parent body.

Howard, K. T.; Benedix, G. K.; Bland, P. A.; Cressey, G.

2010-09-01

139

Formation of biphenyl and dibenzofuran phytoalexins in the transition zones of fire blight-infected stems of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference'.  

PubMed

In the rosaceous subtribe Pyrinae (formerly subfamily Maloideae), pathogen attack leads to formation of biphenyls and dibenzofurans. Accumulation of these phytoalexins was studied in greenhouse-grown grafted shoots of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference' after inoculation with the fire blight bacterium, Erwinia amylovora. No phytoalexins were found in leaves. However, both classes of defence compounds were detected in the transition zone of stems. The flanking stem segments above and below this zone, which were necrotic and healthy, respectively, were devoid of detectable phytoalexins. The transition zone of apple stems contained the biphenyls 3-hydroxy-5-methoxyaucuparin, aucuparin, noraucuparin and 2'-hydroxyaucuparin and the dibenzofurans eriobofuran and noreriobofuran. In pear, aucuparin, 2'-hydroxyaucuparin, noreriobofuran and in addition 3,4,5-trimethoxybiphenyl were detected. The total phytoalexin content in the transition zone of pear was 25 times lower than that in apple. Leaves and stems of mock-inoculated apple and pear shoots lacked phytoalexins. A number of biphenyls and dibenzofurans were tested for their in vitro antibacterial activity against some Erwinia amylovora strains. The most efficient compound was 3,5-dihydroxybiphenyl (MIC=115 ?g/ml), the immediate product of biphenyl synthase which initiates phytoalexin biosynthesis. PMID:22377689

Chizzali, Cornelia; Khalil, Mohammed N A; Beuerle, Till; Schuehly, Wolfgang; Richter, Klaus; Flachowsky, Henryk; Peil, Andreas; Hanke, Magda-Viola; Liu, Benye; Beerhues, Ludger

2012-05-01

140

Characterization of polyphenols and evaluation of antioxidant capacity in grape pomace of the cv. Malbec.  

PubMed

Low molecular weight polyphenols (LMW-PPs) and anthocyanins, along with the antioxidant capacity, were assessed in grape pomace extract (GPE) of red grape (Vitis vinifera L.) cv. Malbec. Twenty-six phenolics (13 LMW-PPs and 13 anthocyanins) were characterized and quantified by HPLC-MWD and UPLC-ESI-MS. The maximum concentrations of LMW-PPs corresponded to the flavanols (+)-catechin and (-)-epicatechin, whereas malvidin-3-glucoside was the most abundant anthocyanin. Piceatannol, a stilbene analogue to resveratrol with higher antioxidant activity, was firstly identified and quantified in GPE of the cv. Malbec. The antioxidant activity for Malbec GPE determined by oxygen radical absorbance capacity (ORAC) assay was 2756?molTEg(-1) GPE. Therefore, the data reported sustain the use of winemaking by-products as a cheap source of phenolic compounds suitable for biotechnological applications, as a strategy for sustainable oenology. PMID:25704698

Antoniolli, Andrea; Fontana, Ariel R; Piccoli, Patricia; Bottini, Rubén

2015-07-01

141

Structural characterization of potato protease inhibitor I (Cv. Bintje) after expression in Pichia pastoris.  

PubMed

In the present study the structural properties of potato protease inhibitor 1 (PI-1) were studied as a function of temperature to elucidate its precipitation mechanism upon heating. A cDNA coding for PI-1 from cv. Bintje was cloned and expressed in Pichia pastoris. Using the recombinant PI-1 it was suggested that PI-1 behaves as a hexameric protein rather than as a pentamer, as previously proposed in the literature. The recombinant protein seems either to have a predominantly unordered structure or to belong to the beta-II proteins. Differential scanning calorimetry analysis of PI-1 revealed that its thermal unfolding occurs via one endothermic transition in which the hexameric PI-1 probably unfolds, having a dimer instead of a monomer as cooperative unit. The transition temperature for the recombinant PI-1 was 88 degrees C. Similar results were obtained for a partially purified pool of native PI-1 from cv. Bintje. PMID:15264937

van den Broek, Lambertus A M; Pouvreau, Laurice; Lommerse, Gijs; Schipper, Bert; Van Koningsveld, Gerrit A; Gruppen, Harry

2004-07-28

142

Graphical method for determining the coefficient of consolidation cv from a flow-pump permeability test  

USGS Publications Warehouse

A graphical method has been developed for determining the coefficient of consolidation from the transient phases of a flow-pump permeability test. The flow pump can be used to infuse fluid into or withdraw fluid from a laboratory sediment specimen at a constant volumetric rate in order to obtain data that can be used to calculate permeability using Darcy's law. Representative type-curve solutions to the associated forced-flow and pressure-decay models are derived. These curves provide the basis for graphically evaluating the permeability k, the coefficient of consolidation cv, and the coefficient of volume change mv. The curve-matching technique is easy and rapid. Values of k, cv and mv for a laterally confined kaolinite specimen were determined by this graphical method and appear to be in reasonably good agreement with numerically derived estimates (within 20%). Discrepancies between the two sets of results seem to be largely a function of data quality.

Morin, Roger H.; Olsen, Harold W.; Nelson, Karl R.; Gill, James D.

1989-01-01

143

Efficient and Stable Transformation of Lactuca sativa L. cv. Cisco (lettuce) Plastids  

Microsoft Academic Search

Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However,\\u000a broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation\\u000a systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene

Hirosuke Kanamoto; Atsushi Yamashita; Hiroshi Asao; Satoru Okumura; Hisabumi Takase; Masahira Hattori; Akiho Yokota; Ken-Ichi Tomizawa

2006-01-01

144

Extremely Na and Cl-rich chondrule from the CV3 carbonaceous chondrite Allende  

Microsoft Academic Search

We report on a study of Al3509, a large Na- and Cl-rich, radially-zoned object from the oxidized CV carbonaceous chondrite Allende. Al3509 consists of fine-grained ferroan olivine, ferroan Al-diopside, nepheline, sodalite, and andradite, and is crosscut by numerous veins of nepheline, sodalite, and ferroan Al-diopside. Some poorly-characterized phases of fine-grained material are also present; these phases contain no significant H2O.

G. J. Wasserburg; I. D. Hutcheon; J. Aléon; E. C. Ramon; A. N. Krot; K. Nagashima; A. J. Brearley

2011-01-01

145

A stable reddish purple anthocyanin in the leaf of Gynura aurantiaca cv. ‘Purple Passion’  

Microsoft Academic Search

The anthocyanin (GAA) in the epidermis and hair of the leaf ofGynura aurantiaca cv. ‘Purple Passion’ was isolated and identified as cyanidin tetra-glucoside acylated by three molecules of caffeic acid\\u000a and one molecule of malonic acid. GAA was also isolated from the lower epidermis of the leaf ofG. bicolor DC. GAA showed a very stable reddish purple color from weakly

Kunijiro Yoshitama; Masahiko Kaneshige; Nariyuki Ishikura; Fukumi Araki; Shoji Yahara; Kenzo Abe

1994-01-01

146

Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis  

Microsoft Academic Search

A protocol for plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis was developed. Viable protoplasts were isolated from embryogenic cell suspensions\\u000a at a yield of 1.2 × 107 protoplasts\\/ml packed cell volume (PCV). Liquid and feeder layer culture systems with medium-A and medium-B were used for\\u000a protoplast culture. In liquid culture system, medium-B was more efficient for

Wang Xiao; Xue-Lin Huang; Xia Huang; Ya-Ping Chen; Xue-Mei Dai; Jie-Tang Zhao

2007-01-01

147

Protein degradation and peptidase activity during petal senescence in Dendrobium cv. Khao Sanan  

Microsoft Academic Search

During ethylene-induced petal senescence in Dendrobium cv. Khao Sanan flowers, the levels of water-insoluble protein in petals decreased but the levels of water-soluble proteins were not affected. Total peptidase activity in the petals increased from day 1 of ethylene treatment and showed a peak by day 4. Treatment of excised flowers with E-64, a specific inhibitor of cysteine peptidases, prior

Ladawan Lerslerwong; Saichol Ketsa; Wouter G. van Doorn

2009-01-01

148

Alkaloid Production in Tissue Cultures of Papaver somniferum L. cv. Office-95  

E-print Network

The capacity of alkaloid synthesis was examined in embryogenic callus tissues of Turkish opium poppy, Papaver somniferum L cv. office-95. Eight months old cultures grown in hormone-free MS were examined for alkaloid content. They were found to produce codeine at a level of 1.2 × 10-2, the baine 2.5 × 10-3, noscapine 2.2 × 10-3, morphine 1.6 × 10-3 and papaverine 5.2 × 10-4 % FW.

Esin Akçam Oluk

149

IV and CV curves for irradiated prototype BTeV silicon pixel sensors  

SciTech Connect

The authors present IV and CV curves for irradiated prototype n{sup +}/n/p{sup +} silicon pixel sensors, intended for use in the BTeV experiment at Fermilab. They tested pixel sensors from various vendors and with two pixel isolation layouts: p-stop and p-spray. Results are based on exposure with 200 MeV protons up to 6 x 10{sup 14} protons/cm{sup 2}.

Maria R. Coluccia et al.

2002-07-16

150

Heavily metamorphosed clasts from the CV chondrite breccias Mokoia and Yamato-86009  

NASA Astrophysics Data System (ADS)

Abstract- Metamorphosed clasts in the CV carbonaceous chondrite breccias Mokoia and Yamato-86009 (Y-86009) are coarse-grained, granular, polymineralic rocks composed of Ca-bearing (up to 0.6 wt% CaO) ferroan olivine (Fa34-39), ferroan Al-diopside (Fs9-13Wo47-50, approximately 2-7 wt% Al2O3), plagioclase (An37-84Ab63-17), Cr-spinel (Cr/(Cr + Al) = 0.19-0.45, Fe/(Fe + Mg) = 0.60-0.79), nepheline, pyrrhotite, pentlandite, Ca-phosphate, and rare grains of Ni-rich taenite; low-Ca pyroxene is absent. Most clasts have triple junctions between silicate grains, indicative of prolonged thermal annealing. Based on the olivine-spinel and pyroxene thermometry, the estimated metamorphic temperature recorded by the clasts is approximately 1100 K. Few clasts experienced thermal metamorphism to a lower degree and preserved chondrule-like textures. The Mokoia and Y-86009 clasts are mineralogically unique and different from metamorphosed chondrites of known groups (H, L, LL, R, EH, EL, CO, CK) and primitive achondrites (acapulcoites, brachinites, lodranites). On a three-isotope oxygen diagram, compositions of olivine in the clasts plot along carbonaceous chondrite anhydrous mineral line and the Allende mass-fractionation line, and overlap with those of the CV chondrule olivines; the ?17O values of the clasts range from about -4.3‰ to -3.0‰. We suggest that the clasts represent fragments of the CV-like material that experienced metasomatic alteration, high-temperature metamorphism, and possibly melting in the interior of the CV parent asteroid. The lack of low-Ca pyroxene in the clasts could be due to its replacement by ferroan olivine during iron-alkali metasomatic alteration or by high-Ca ferroan pyroxene during melting under oxidizing conditions.

Jogo, Kaori; Nagashima, Kazuhide; Hutcheon, Ian D.; Krot, Alexander N.; Nakamura, Tomoki

2012-12-01

151

Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO  

Microsoft Academic Search

Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent\\u000a field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity\\u000a of the tissues involved. Factors causing these variations were investigated by climate

Andreas Albert; Vipaporn Sareedenchai; Werner Heller; Harald K. Seidlitz; Christian Zidorn

2009-01-01

152

The Influence of Fungicide Sprays on Infection Of Apple cv. Bramley's Seedling By Nectria galligena  

Microsoft Academic Search

In 1990, a long-term trial was initiated by planting young apple trees, cv. Bramley's Seedling, inoculated, at single sites in the leader shoots, with Nectria galligena. The effect of spring–summer and autumn fungicide spray programmes, applied during 1991–1993, on the development of new cankers was assessed up to May 1994. Spring–summer fungicide programmes, applied as for the control of apple

Louise R. Cooke

1999-01-01

153

Establishment of callus and cell suspension cultures of raspberry (Rubus idaeus cv. Royalty)  

Microsoft Academic Search

Callus cultures were initiated from leaf sections of raspberry (Rubus idaeus L.) cv. Royalty. Explants from younger leaves produced significantly more calli than those from older leaves. Anderson's salt mixture was more efficient for callus induction than the Murashige-Skoog medium. The best propagation and growth of calli was observed on Anderson's medium supplemented with 9 µM 2,4-dichlorophenoxyacetic acid, 4.9 µM

Wledzimierz Berejsza-Wysecki; Geza Hrazdin

1994-01-01

154

Effects of cycocel on growth and seed yield of Lotus uliginosus Schk. cv. Grasslands Maku  

Microsoft Academic Search

Field experiments were conducted in two successive seasons to investigate the effects of the plant growth retardant cycocel applied at 1.25 and 2.50 kg a.i.\\/ha at different plant growth stages on the vegetative and reproductive growth, yield components, and seed yield of Lotus uliginosus Schk. cv. Grasslands Maku. Cycocel did not retard internode length or affect total dry matter, flowering

R. S. Tabora; J. G. Hampton

1992-01-01

155

In vitro culture of Safflower L. cv. Bhima: initiation, growth optimization and organogenesis  

Microsoft Academic Search

Callus induction and in vitro plantlet regeneration systems for safflower (Carthamus tinctorius L.) cv. Bhima using root,\\u000a hypocotyl, cotyledon and leaf explants were optimized by studying the influence on organogenesis of seedling age, media factors,\\u000a growth regulators and excision orientation. Supplementation of the medium with an auxin: cytokinin ratio < 1 enhanced the\\u000a growth rate of callus cultures; however, for

T. D. Nikam; M. G. Shitole

1998-01-01

156

Changes in leaf, stem, and root anatomy of Chrysanthemum cv. Lillian Hoek following paclobutrazol application  

Microsoft Academic Search

Plants ofChrysanthemum cv. Lillian Hoek were treated with a paclobutrazol (PBZ) soil drench and histologically examined after 3 months. PBZ application\\u000a resulted in thicker leaves, reduced stem diameter, and roots with an increased diameter and an unusual segmented appearance.\\u000a Increased leaf thickness was partly due to an additional layer of palisade mesophyll, although individual palisade cells were\\u000a shorter, of smaller

G. E. Burrows; T. S. Boag; W. P. Stewart

1992-01-01

157

Phylogenetic position of Mesorhizobium huakuii subsp. rengei, a Symbiont of Astragalus sinicus cv. Japan  

Microsoft Academic Search

The phylogenetic position of Rhizobium huakuii bv. renge, a symbiont of Astragalus sinicus cv. Japan (renge-sou), was studied. The following phylogenetic approaches were used: restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR) analysis of a full-length 16S rDNA fragment, 16S rDNA analysis of the first 300-bp sequence, bacteriophage typing, and amplification of the genomic region by random primer. All the

Sukma Nuswantara; Makoto Fujie; Takashi Yamada; Wanda Malek; Masaki Inaba; Yoshinobu Kaneko; Yoshikatsu Murooka

1999-01-01

158

An accurate method of 6H-SiC PIN structures parameter extraction using CV characteristics  

Microsoft Academic Search

Our paper reports an optimal parameter extraction method based on a new model for the high frequency capacitance-voltage characteristics of the 6H-SiC boron doped junction. The C-V model confirms the presence of two type regions (p- and n-) in the quasi-intrinsic layer induced by boron doping. The extracted values of the net doping of these zones (6-10×1012 cm-3) are in

B. Tudor; G. Brezeanul; M. Badila; M. L. Locatelli; J. P. Chante; J. Millan; Ph. Godignon; A. Lebedev; N. S. Savkina

1998-01-01

159

SALT spectral observations of a new, bright, southern CV: MASTER OT J142023.5-485540  

NASA Astrophysics Data System (ADS)

On 2013-06-24.063 UT, the Southern African Large Telescope (SALT; Buckley et al. 2006; O’Donoghue et al. 2006) observed MASTER OT J142023.5-485540 (RA=14h 20m 23.5s; Dec=-48d 55m 40s). This outbursting CV was discovered by MASTER-ICATE on 2013-06-08.048 UT and followup observations were requested in ATEL #5144.

Gulbis, A. A. S.; Kotze, M. M.; Kotze, E. J.; Worters, H. L.; Buckley, D. A. H.; O'Donoghue, H.; Shara, M.

2013-07-01

160

Blanching effects on chemistry, quality and structure of green beans (cv. Moncayo )  

Microsoft Academic Search

Green beans (cv. Moncayo) were blanched at 65, 70, 75, 80, 85, 90 and 97 °C for 2.5, 5, 10, 20 and 40 min. Pectinesterase (PE) activity was highest in cell-wall-bound extracts of beans blanched at 70 °C\\/10 min. The lowest water-soluble pectin fraction, the highest EDTA-soluble pectin fraction and the lowest degree of esterification of the EDTA-soluble fraction were all recorded for the

Wenceslao Canet; María Dolores Alvarez; Pilar Luna; Cristina Fernández; María Estrella Tortosa

2005-01-01

161

The calibration of a binocular intersection VMD measurement system based on OpenCV  

Microsoft Academic Search

In this paper we present a two-step stereo calibration method of binocular intersection VMD measurement system based on OpenCV. First, calibrate the two cameras separately, and get the camera's intrinsic parameters. Second, solve the extrinsic parameters from the stereo image pairs and the camera's intrinsic parameters. The experiment shows that this two-step calibration method for binocular intersection VMD measurement system

Yang Zhenhua; He Lingsong; Bei Lei

2010-01-01

162

Study on on-line measurement system of stone slabs based on OpenCV  

Microsoft Academic Search

An on-line measurement system of stone slabs based on CCD technology is constituted by using the CCD camera, image acquisition card, computer and so on. The on-line measurement system for the stone slab is realized by applying camera calibration and image processing algorithms. Under VC++6.0 condition, camera calibration is realized by calling OpenCV1.0 library function and stone slab contour is

Zhao Min; Na Lihong

2010-01-01

163

Highly optimized implementation of OpenCV for the Cell Broadband Engine  

Microsoft Academic Search

Recently, real-time processing of image recognition is required for embedded applications such as automotive applications, robotics, entertainment, and so on. To realize real-time processing of image recognition on such systems we need optimized libraries for embedded processors. OpenCV is one of the most widely used libraries for computer vision applications and has many functions optimized for Intel processors, but no

Hiroki Sugano; Ryusuke Miyamoto

2010-01-01

164

Research of Driver Eye Features Detection Algorithm Based on OpenCV  

Microsoft Academic Search

This paper proposes a driver fatigue eye features detection algorithm based on OpenCV image processing and computer vision development platform. This algorithm localizes eye-area and detects its state based on rough to accurate thought, and can localize eye pupils in eye-open state accurately, which has significance to decrease traffic accidents. The experiment shows this algorithm can detect drivers' eye states

Shifeng Hu; Zuhua Fang; Jie Tang; Hongbing Xu; Ying Sun

2010-01-01

165

Nature and origin of interstellar diamond from the Allende CV3 meteorite  

Microsoft Academic Search

Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende CV3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on

David Blake; Friedemann Freund; Ted Bunch; Kannan Krishnan; Mitch Stampfer; Sherwood Chang; Alexander G. G. M. Tielens

1990-01-01

166

Requirements for plant regeneration from protoplasts of the shrubby ornamental honeysuckle, Lonicera nitida cv. Maigrun  

Microsoft Academic Search

Leaf protoplasts of axenic shoot cultures of Lonicera nitida cv Maigrun underwent sustained division to give multicellular colonies (microcalli) on a modified, ammonium-free MS (Murashige & Skoog) medium containing 0.5 mg l-1 NAA (1-naphthaleneacetic acid), 1.0 mg l-1 BAP (6-benzylaminopurine) and 150 mg l-1 casein enzymatic hydrolysate. Callus was produced upon transfer of cell colonies to MS medium with 2.0

S. J. Ochatt

1991-01-01

167

Cloning, expression and immunolocalization pattern of a cinnamyl alcohol dehydrogenase gene from strawberry (Fragariaxananassa cv. Chandler)  

Microsoft Academic Search

Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) catalyses the conversion of p-hydroxy-cinnamalde- hydes to the corresponding alcohols and is con- sidered a key enzyme in lignin biosynthesis. By a differential screening of a strawberry (Fragaria3 ananassa cv. Chandler) fruit specific subtractive cDNA library, a full-length clone corresponding to a cad gene was isolated (Fxacad1). Northern blot and quantitative real time PCR

R. Blanco-Portales; N. Medina-Escobar; J. A. Lopez-Raez; J. A. Gonzalez-Reyes; J. M. Villalba; E. Moyano; J. L. Caballero; J. Munoz-Blanco

2002-01-01

168

Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds  

Microsoft Academic Search

Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain\\u000a bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic\\u000a solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest

Pedro Chávez-Quintal; Tania González-Flores; Ingrid Rodríguez-Buenfil; Santiago Gallegos-Tintoré

2011-01-01

169

Anthelmintic activities of aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana.  

PubMed

Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana. PMID:24583851

Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

2014-01-01

170

Umbilical reconstruction using a modified inverted C-V flap with conjoint flaps.  

PubMed

The umbilicus is an important aesthetic feature of the abdomen. Because of its location, the umbilicus can be injured after abdominal surgical procedures. Various methods have been devised to reconstruct the umbilicus by using local flaps, purse-string sutures, or a cartilage graft, but there are no ideal methods. The authors have created a modified inverted C-V flap with conjoint flaps. A 10-year-old boy presented with deformed umbilicus because he had undergone surgical correction of an omphalocele. The drawback of the traditional C-V flap method is the transverse long abdominal scar because of the long length of the V flap. However, by using two conjoint flaps at the superior part of the C-V flap, the length of V flap can be more short and the umbilical wall can be reconstructed by rotation of two conjoint flaps. It is also good for making a sinusoidal pocket and it makes the umbilicus deeper and more natural-looking. After the operation, there were no complications like flap necrosis, infection, haematoma, and so on. The patient was satisfied with the results The patient had a more attractive umbilicus than the one with the other previous technique. This new method makes a natural-looking umbilicus with less of a transverse scar and an adequate sinusoidal pocket and umbilical wall. PMID:23829506

Lee, Yoonho; Lee, San Ha; Woo, Kevin Volt

2013-09-01

171

RXJ1313.2-3259, a missing link in CV evolution?  

E-print Network

We present low-state IUE spectroscopy of the ROSAT-discovered polar RXJ1313.2-3259. The SWP spectrum displays a broad Lyman alpha absorption profile, which can be fitted with a two-temperature model of a white dwarf of Twd=15000K with a hot spot of Tspot=34000K which covers f~0.01 of the white dwarf surface. The white dwarf temperature is atypically low for the long orbital period (4.18h) of RXJ1313.2-3259. This low temperature implies either that the system is a young CV in the process of switching on mass transfer or that it is an older CV found in a prolonged state of low accretion rate, much below that predicted by standard evolution theory. In the first case, we can put a lower limit on the life time as pre-CV of 10e8yrs. In the second case, the good agreement of the white dwarf temperature with that expected from compressional heating suggests that the system has experienced the current low accretion rate for an extended period >10e4yrs. A possible explanation for the low accretion rate is that RXJ1313.2-3259 is a hibernating post nova and observational tests are suggested.

B. T. Gaensicke; K. Beuermann; D. de Martino; H. -C. Thomas

1999-10-25

172

Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana  

PubMed Central

Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (?)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana. PMID:24583851

Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

2014-01-01

173

Teaching image processing and pattern recognition with the Intel OpenCV library  

NASA Astrophysics Data System (ADS)

In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image processing and pattern recognition with the most up-to-date solutions available. In the Institute of Electronics at the Technical University of Lodz we facilitate the teaching process in this subject with the OpenCV library, which is an open-source set of classes, functions and procedures that can be used in programming efficient and innovative algorithms for various purposes. The topics of student projects completed with the help of the OpenCV library range from automatic correction of image quality parameters or creation of panoramic images from video to pedestrian tracking in surveillance camera video sequences or head-movement-based mouse cursor control for the motorically impaired.

Koz?owski, Adam; Królak, Aleksandra

2009-06-01

174

Modal Mineralogy of CV3 Chondrites by PSD-XRD: Mineralogic Insights into a Complex Evolutionary History  

NASA Astrophysics Data System (ADS)

CV3 chondrites Vigarano, Efremovka, Allende, Mokoia, Grosnaja and Kaba are amongst the most studied rocks in existence. By XRD we define the first quantitative modal mineralogy of these samples and explore implications of our data to petrogenesis.

Howard, K. T.; Benedix, G. K.; Bland, P. A.; Cressey, G.

2009-03-01

175

Antihypertensive effect of CV-4093.2HCl, a new calcium antagonist, in three rat models of hypertension.  

PubMed

The hypotensive action of CV-4093.2HCl (CV-4093), a new calcium antagonist, was studied in spontaneously hypertensive, renal hypertensive, DOCA-salt hypertensive and normotensive rats. CV-4093 (3 and 10 mg/kg, p.o.) dose-dependently decreased systolic blood pressure in the three types of hypertensive rats. At the dose of 10 mg/kg, the compound decreased the blood pressure to the normotensive level between 1 and 3 hr after it was administered; the antihypertensive effect lasted for at least 8 hr. The systolic blood pressure in normotensive rats was also decreased at 3 and 10 mg/kg, p.o., but less evidently than in the hypertensive rats. When the antihypertensive effect of CV-4093 was compared with that of seven known calcium antagonists in spontaneously hypertensive rats, it was the most potent and the most long-lasting. PMID:3210447

Kakihana, M; Suno, M; Nagaoka, A

1988-10-01

176

Defective secretion of mucilage is the cellular basis for agravitropism in primary roots of Zea mays cv. Ageotropic  

NASA Technical Reports Server (NTRS)

Root caps of primary, secondary, and seminal roots of Z. mays cv. Kys secrete large amounts of mucilage and are in close contact with the root all along the root apex. These roots are strongly graviresponsive. Secondary and seminal roots of Z. mays cv. Ageotropic are also strongly graviresponsive. Similarly, their caps secrete mucilage and closely appress the root all along the root apex. However, primary roots of Z. mays cv. Ageotropic are non-responsive to gravity. Their caps secrete negligible amounts of mucilage and contact the root only at the extreme apex of the root along the calyptrogen. These roots become graviresponsive when their tips are coated with mucilage or mucilage-like materials. Peripheral cells of root caps of roots of Z. mays cv. Kys contain many dictyosomes associated with vesicles that migrate to and fuse with the plasmalemma. Root-cap cells of secondary and seminal (i.e. graviresponsive) roots of Z. mays cv. Ageotropic are similar to those of primary roots of Z. mays cv. Kys. However, root-cap cells of primary (i.e. non-graviresponsive) roots of Z. mays cv. Ageotropic have distended dictyosomal cisternae filled with an electron-dense, granular material. Large vesicles full of this material populate the cells and apparently do not fuse with the plasmalemma. Taken together, these results suggest that non-graviresponsiveness of primary roots of Z. mays cv. Ageotropic results from the lack of apoplastic continuity between the root and the periphery of the root cap. This is a result of negligible secretion of mucilage by cells along the edge of the root cap which, in turn, appears to be due to the malfunctioning of dictyosomes in these cells.

Miller, I.; Moore, R.

1990-01-01

177

Using multidimensional Rasch analysis to validate the Chinese version of the Motivated Strategies for Learning Questionnaire (MSLQ-CV)  

Microsoft Academic Search

This article used the multidimensional random coefficients multinomial logit model to examine the construct validity and detect\\u000a the substantial differential item functioning (DIF) of the Chinese version of motivated strategies for learning questionnaire\\u000a (MSLQ-CV). A total of 1,354 Hong Kong junior high school students were administered the MSLQ-CV. Partial credit model was\\u000a suggested to have a better goodness of fit

John Chi-Kin Lee; Zhonghua Zhang; Hongbiao Yin

2010-01-01

178

My CV  

E-print Network

Tong Liu. Current Address: E-Mail: ... Research Interests: Arithmetic geometry, algebraic number theory, p-adic Galois ... p=2, to appear, Journal de Théroie des Nombres de Bordeaux. [10] A.O.L ... International Mathematics Research Notices.

tong&zhiyi

2015-01-24

179

Compositional and petrographic similarities of CV and CK chondrites: A single group with variations in textures and volatile concentrations attributable to impact heating, crushing and oxidation  

NASA Astrophysics Data System (ADS)

Greenwood et al. (2010) gathered data on O-isotopic and elemental compositions and reevaluated literature data for CV and CK chondrites. They concluded that these two chondrite groups originated on the same parent asteroid, with CK chondrites being metamorphosed CV chondrites (which are otherwise missing types 4 through 6). To test this interpretation we have gathered new instrumental neutron-activation-analysis (INAA) data for CV and CK chondrites and reexamined their petrographic features. The new INAA data like the older data show scatter attributable to weathering effects, but we conclude that the refractory lithophile abundances are the same in CV and CK, in agreement with the Greenwood et al. interpretation. Several volatile elements are significantly lower in CK than in CV chondrites. Among the elements we determine, the greatest difference between CV and CK is found for Br, for which the CV/CK ratio is ?4; As and Sb are about 20% lower in CK than CV and smaller differences are observed for Zn, Ga and Se. It seems likely that volatiles were lost during impact-heating events that also provided the heat responsible for metamorphic recrystallization. Within statistical uncertainty, chondrules in CV and CK chondrites are the same size and have similar textural distributions. A significant petrographic difference between CK and CV chondrites cited by Kallemeyn et al. (1991) was the much higher percentage of igneous rims around CV chondrules. However, we now recognize that many chondrules in CK3.8 NWA 1559 have igneous rims and in CK4 chondrites, igneous rims are recognizable by their associated sulfide-rich rings; there are no quantifiable CV-CK differences in igneous-rim abundances. We used Ca and Al maps to show that CK chondrites have CAI abundances similar to those of CV chondrites. It thus appears that there are no resolvable pre-metamorphic petrographic differences between CV and CK chondrites. We recommend that the “CK” designation be abandoned and that the CV group be acknowledged as spanning the range of petrologic types 3-6. We suggest that CK3 chondrites be designated CV3OxK. Most CK4 chondrites are highly fragmental; collisional crushing appears to be much more common than in CV chondrites. It seems likely that CK chondrites formed from reduced CV3 materials after the latter was impacted, buried, aqueously altered and annealed.

Wasson, John T.; Isa, Junko; Rubin, Alan E.

2013-05-01

180

Unusual dark clasts in the Vigarano CV3 carbonaceous chondrite: Record of parent body process  

NASA Astrophysics Data System (ADS)

Two unusual dark clasts found in the Vigarano CV3 chondrite were examined using an optical microscope and a scanning electron microscope (SEM). Both clasts lack chondrules, Ca-Al-rich inclusions, and coarse-grained mineral fragments; they, instead, contain abundant inclusions that consist of fine grains (less than 1 micrometer) of homogeneous Fe-rich olivine, thus resembling the fine-grained variety of dark inclusions in CV3 chondrites. The external shapes of inclusions in the clasts bear a close resemblance to those of chondrules and chondrule fragments; some of the inclusions are surrounded by dark rims similar to chondrule rims. Our SEM observations reveal the following unusual characteristics: 1) the inclusions are not mere random aggregates of olivine grains but have peculiar internal textures, that is, assemblages of round or oval shaped outlines, which are suggestive of pseudomorphs after porphyritic olivine chondrules; 2) one of thick inclusion rims contains a network of vein-like strings of elongated olivine grains; 3) an Fe-Ni metal aggregate in one of the clasts has an Fe-, Ni-, S-rich halo suggesting a reaction between its precursor and the surrounding matrix; and 4) olivine in the clasts commonly shows a swirly, fibrous texture similar to that of phyllosilicate. These characteristics suggest that the dark clasts in Vigarano are not primary aggregates of dust in the solar nebula but were affected by aqueous alteration and subsequent dehydration by heating after accretion to the meteorite parent body. The fine olivine grains in these clasts were presumably produced by thermal transformation of phyllosilicate, as is the case with those in the two thermally metamorphosed Antarctic CM chondrites, Belgica-7904 and Yamato-86720. From textural and mineralogical similarities, some of the dark inclusions and clasts previously reported from CV3 chondrites and other types of meteorites may have origins common with these clasts in Vigarano.

Kojima, Tomoko; Tomeoka, Kazushige; Takeda, Hiroshi

1993-12-01

181

Potato virus Y CFH, a putative recombinant isolate from Capsicum chinense cv. Habanero.  

PubMed

Ornamental plants of Chili pepper, Capsicum chinense cv. Habanero, with symptoms of leaf mosaic, necrotic rings on fruits and necrotic stems were observed in June 2003 in a private garden in the province of Naples (Italy). Preliminary serological characterisation allowed the association of these symptoms with infections by Potato virus Y (PVY). The virus was isolated on Nicotiana tabacum cv. Xanthi and characterised by mechanical inoculation on herbaceous hosts and molecular characterisation of the P1 and the coat protein (CP) genes. Symptoms produced on indicator plants were generally consistent with those described for PVY. The identity of PVY was further confirmed by reaction with PVYN, PVYC and PVYO specific monoclonal antibodies: the isolate reacted only with the PVYC specific Mab. Immuno capture reverse transcription polymerase chain reaction (IC-RT-PCR) was performed on extracts of PVY-CFH infected N. tabacum cv. Xanthi plants, using two couples of primers specifically designed out of the P1 and the CP coding regions of the so far fully sequenced PVY isolates. PCR products were then cloned into pCRII-TOPO vector using TOPO-TA cloning kit (Invitrogen) and sequenced. Sequence analysis suggests that PVY-CFH originated from a recombination event involving a virus of the PVYO type and another parental virus, maybe resembling the PVYNP isolates, given the reasonably high similarity shared by PVY-CFH and, respectively, non potato PVY isolates in the CP coding region, PVYO isolates in the P1 coding region. Evidence for the existence of such a recombination comes, apart from similarity analysis, by the different locations of CFH within phylogenetic trees constructed from P1 and CP genomic regions. PMID:17390887

Comes, S; Fanigliulo, A; Pacella, R; Parrella, G; Crescenzi, A

2006-01-01

182

Carrier Density Profiling of Ultra-Shallow Junction Layers Through Corrected C-V Plotting  

SciTech Connect

The aim of this report is to present and justify a new approach for carrier density profiling in ultra-shallow junction (USJ) layer. This new approach is based on a capacitance measurement model, which takes series impedance, shunt resistance and the presence of a boron skin on the USJ layer into account. It allows us to extract the depletion layer capacitances in the USJ layer from C-V plotting more accurately and hence to obtain better carrier density profiles. Based on this new approach the carrier density profiles of different USJ layers with and without halo-style implants are obtained and discussed.

Chen, James; Dimitrov, Dimitar; Dimitrova, Tatiana [Four Dimensions, Inc., 3140 Diablo Ave, Hayward, California, 94545 (United States); Timans, Paul [Mattson Technology, Inc. Fremont, California (United States); Gelpey, Jeff; McCoy, Steve [Mattson Technology Canada, Inc., Vancouver (Canada); Lerch, Wilfried; Paul, Silke [Mattson Thermal Products GmbH, Dornstadt (Germany); Bolze, Detlef [IHP, Frankfurt (Oder) (Germany)

2008-11-03

183

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical  

Microsoft Academic Search

A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and\\u000a Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1–1.0 mg\\/l), ?-naphthalene\\u000a acetic acid (0.01–0.2 mg\\/l), thidiazuron (0.5–2.0 mg\\/l), 6-benzylaminopurine (0.5–1.0 mg\\/l), and kinetin (0.5–1.0 mg\\/l)] were\\u000a tested for their ability

Budi Winarto; F. Rachmawati

184

Serine protease inhibitor cvSI-1 potential role in the eastern oyster host defense against the protozoan parasite Perkinsus marinus.  

PubMed

The serine protease inhibitor cvSI-1, purified from plasma of eastern oysters, inhibited the proliferation of the protozoan parasite Perkinsus marinus in vitro. In situ hybridization located cvSI-1 gene expression in basophil cells of the digestive tubules and cvSI-1 expression measured by real-time quantitative reverse transcriptase polymerase chain reaction was several hundred folds greater in digestive glands than in other organs examined or circulating hemocytes. cvSI-1 gene expression was also significantly greater in winter than in summer. Finally, cvSI-1 gene expression and plasma protease inhibitory activity in oysters selected for increased resistance to P. marinus were significantly greater than in unselected oysters. These findings support the hypothesis that cvSI-1 plays a role in eastern oyster host defense against P. marinus possibly through inhibition of parasite proliferation. PMID:19720077

La Peyre, Jerome F; Xue, Qing-Gang; Itoh, Naoki; Li, Yanli; Cooper, Richard K

2010-01-01

185

Heterogeneous compute in computer vision: OpenCL in OpenCV  

NASA Astrophysics Data System (ADS)

We explore the relevance of Heterogeneous System Architecture (HSA) in Computer Vision, both as a long term vision, and as a near term emerging reality via the recently ratified OpenCL 2.0 Khronos standard. After a brief review of OpenCL 1.2 and 2.0, including HSA features such as Shared Virtual Memory (SVM) and platform atomics, we identify what genres of Computer Vision workloads stand to benefit by leveraging those features, and we suggest a new mental framework that replaces GPU compute with hybrid HSA APU compute. As a case in point, we discuss, in some detail, popular object recognition algorithms (part-based models), emphasizing the interplay and concurrent collaboration between the GPU and CPU. We conclude by describing how OpenCL has been incorporated in OpenCV, a popular open source computer vision library, emphasizing recent work on the Transparent API, to appear in OpenCV 3.0, which unifies the native CPU and OpenCL execution paths under a single API, allowing the same code to execute either on CPU or on a OpenCL enabled device, without even recompiling.

Gasparakis, Harris

2014-02-01

186

Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO.  

PubMed

Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity of the tissues involved. Factors causing these variations were investigated by climate chamber (CC) experiments focusing on temperature and ultraviolet (UV)-B radiation. Plants of A. montana L. cv. ARBO were grown in CCs under realistic climatic and radiation regimes. Key factors temperature and UV-B radiation were altered between different groups of plants. Subsequently, flowering heads were analyzed by HPLC for their contents of flavonoids and caffeic acid derivatives. Surprisingly, increased UV-B radiation did not trigger any change in phenolic metabolites in Arnica. In contrast, a pronounced increase in the ratio of B-ring ortho-diphenolic (quercetin) compared to B-ring monophenolic (kaempferol) flavonols resulted from a decrease in temperature by 5 degrees C in the applied climate regime. In conclusion, enhanced UV-B radiation is probably not the key factor triggering shifts in the phenolic composition in Arnica grown at higher altitudes but rather temperature, which decreases with altitude. PMID:19194724

Albert, Andreas; Sareedenchai, Vipaporn; Heller, Werner; Seidlitz, Harald K; Zidorn, Christian

2009-05-01

187

Plasmalemmal vesicle associated protein (PV1) modulates SV40 virus infectivity in CV-1 cells  

PubMed Central

Plasmalemmal vesicle associated protein (Plvap/PV1) is a structural protein required for the formation of the stomatal diaphragms of caveolae. Caveolae are plasma membrane invaginations that were implicated in SV40 virus entry in primate cells. Here we show that de novo Plvap/PV1 expression in CV-1 green monkey epithelial cells significantly reduces the ability of SV40 virus to establish productive infection, when cells are incubated with low concentrations of the virus. However, in presence of high viral titers PV1 has no effect on SV40 virus infectivity. Mechanistically, PV1 expression does not reduce the cell surface expression of known SV40 receptors such as GM1 ganglioside and MHC class I proteins. Furthermore, PV1 does not reduce the binding of virus-like particles made by SV40 VP1 protein to the CV-1 cell surface and does not impact their internalization when cells are incubated with either high or low VLP concentrations. These results suggest that PV1 protein is able to block SV40 infectivity at low but not at high viral concentration either by interfering with the infective internalization pathway at the cell surface or at a post internalization step. PMID:21827737

Tse, Dan; Armstrong, David A.; Oppenheim, Ariella; Kuksin, Dimitry; Norkin, Leonard; Stan, Radu V.

2011-01-01

188

Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds.  

PubMed

Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative identification of the compounds present in the lowest and highest yield extracts. Analysis of the leaf extract with phytochemical tests showed the presence of alkaloids, flavonoids and terpenes. Antifungal effectiveness was determined by challenging the extracts (LE, SRE, SUE) from the best extraction treatment against three phytopathogenic fungi: Rhizopus stolonifer, Fusarium spp. and Colletotrichum gloeosporioides. The leaf extract exhibited the broadest action spectrum. The MIC(50) for the leaf extract was 0.625 mg ml(-1) for Fusarium spp. and >10 mg ml(-1) for C. gloeosporioides, both equal to approximately 20% mycelial growth inhibition. Ethanolic extracts from Carica papaya L. cv. Maradol leaves are a potential source of secondary metabolites with antifungal properties. PMID:22282629

Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago

2011-01-01

189

Antioxidant activity and chemical composition of Torreya grandis cv. Merrillii seed.  

PubMed

Torreya grandis cv. Merrillii is an endemic tree species in China, seeds of which are used as a popular snack, possessing beneficial effects on preventing angiosclerosis and coronary heart diseases. In this study, antioxidant activity and chemical constituents of T. grandis cv. Merrillii seed (TGMS) were investigated. The antioxidant activity of different fractions and the ethanol extract was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, hydroxyl radical scavenging activity and lipid peroxidation assays. The oil, CH2Cl2 and n-BuOH fractions, and ethanol extract of TGMS all showed antioxidant activities in these models, especially the DPPH one. By GC-MS analysis, twenty-seven constituents were identified from the oil fraction of TGMS. The total content of phenolic compounds in the CH2Cl2 and n-BuOH fractions and ethanol extract was also determined by the Folin-Ciocalteau method as 17.6, 21.6 and 12.9 microg/mg, respectively. In addition, analysis of the CH2Cl2 fraction yielded four phenolic compounds: 4-hydroxybenzaldehyde, 4-methoxy pyrocatechol, coniferyl aldehyde, 4-hydroxy cinnamaldehyde, and two steroids, beta-sitosterol and daucosterol. These results provide scientific support for the empirical use of TGMS as a medicinal food for cardiovascular diseases. PMID:19967992

Shi, Haiming; Wang, Huandi; Wang, Mengyue; Li, Xiaobo

2009-11-01

190

Antioxidant and anticancer aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena.  

PubMed

Fifteen compounds were extracted and purified from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena. These compounds include liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) cepharadione B (11), ?-sitostenone (12), stigmasta-4,22-dien-3-one (13) and two chlorophylls: pheophytin-a (14) and aristophyll-C (15). The anti-oxidation activity of the compounds was examined by antiradical scavenging, metal chelating and ferric reducing power assays. The results have shown that these compounds have antioxidative activity. The study has also examined the antiproliferation activity of the isolated compounds against human melanoma, prostate and gastric cancer cells. The results shown that 7-hydroxydehydronuciferine (10) significantly inhibited the proliferation of melanoma, prostate and gastric cancer cells. Together, these findings suggest that leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena are a good resource for obtaining the biologically active substances with antioxidant properties. PMID:25372397

Liu, Chi-Ming; Kao, Chiu-Li; Wu, Hui-Ming; Li, Wei-Jen; Huang, Cheng-Tsung; Li, Hsing-Tan; Chen, Chung-Yi

2014-01-01

191

Multi-camera calibration based on openCV and multi-view registration  

NASA Astrophysics Data System (ADS)

For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the corresponding relationship between each camera view, the computation of the rotation matrix and translation matrix is formulated as a constrained optimization problem. According to the Kuhn-Tucker theorem and the properties on the derivative of the matrix-valued function, the formulae of rotation matrix and translation matrix are deduced by using singular value decomposition algorithm. Afterwards an iterative method is utilized to get the entire coordinate transformation of pair-wise views, thus the precise multi-view registration can be conveniently achieved and then can get the relative positions in them(the camera outside the parameters).Experimental results show that the method is practical in multi-camera calibration .

Deng, Xiao-ming; Wan, Xiong; Zhang, Zhi-min; Leng, Bi-yan; Lou, Ning-ning; He, Shuai

2010-10-01

192

Biochemical markers assisted screening of Fusarium wilt resistant Musa paradisiaca (L.) cv. puttabale micropropagated clones.  

PubMed

An efficient protocol was standardized for screening of panama wilt resistant Musa paradisiaca cv. Puttabale clones, an endemic cultivar of Karnataka, India. The synergistic effect of 6-benzyleaminopurine (2 to 6 mg/L) and thidiazuron (0.1 to 0.5 mg/L) on MS medium provoked multiple shoot induction from the excised meristem. An average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/L 6-benzyleaminopurine and 0.3 mg/L thidiazuron concentrations. Elongation of shoots observed on 5 mg/L BAP augmented medium with a mean length of 8.38 +/- 0.30 shoots per propagule. For screening of disease resistant clones, multiple shoot buds were mutated with 0.4% ethyl-methane-sulfonate and cultured on MS medium supplemented with Fusarium oxysporum f. sp. cubense (FOC) culture filtrate (5-15%). Two month old co-cultivated secondary hardened plants were used for screening of disease resistance against FOC by the determination of biochemical markers such as total phenol, phenylalanine ammonia lyase, oxidative enzymes like peroxidase, polyphenol oxidase, catalase and PR-proteins like chitinase, beta-1-3 glucanase activities. The mutated clones of M. paradisiaca cv. Puttabale cultured on FOC culture filtrate showed significant increase in the levels of biochemical markers as an indicative of acquiring disease resistant characteristics to FOC wilt. PMID:23898552

Venkatesh; Krishna, V; Kumar, K Girish; Pradeepa, K; Kumar, S R Santosh; Kumar, R Shashi

2013-07-01

193

Chemical and physical studies of type 3 chondrites 12: The metamorphic history of CV chondrites and their components  

NASA Technical Reports Server (NTRS)

The induced thermoluminescence (TL) properties of 16 CV and CV-related chondrites, four CK chondrites and Renazzo (CR2) have been measured in order to investigate their metamorphic history. The petrographic, mineralogical and bulk compositional differences among the CV chondrites indicate that the TL sensitivity of the approximately 130 C TL peak is reflecting the abundance of ordered feldspar, especially in chondrule mesostasis, which in turn reflects parent-body metamorphism. The TL properties of 18 samples of homogenized Allende powder heated at a variety of times and temperatures, and cathodoluminescence mosaics of Axtell and Coolidge, showed results consistent with this conclusion. Five refractory inclusions from Allende, and separates from those inclusions, were also examined and yielded trends reflecting variations in mineralogy indicative of high peak temperatures (either metamorphic or igneous) and fairly rapid cooling. The CK chondrites are unique among metamorphosed chondrites in showing no detectable induced TL, which is consistent with literature data that suggests very unusual feldspar in these meteorites. Using TL sensitivity and several mineral systems and allowing for the differences in the oxidized and reduced subgroups, the CV and CV-related meteorites can be divided into petrologic types analogous to those of the ordinary and CO type 3 chondrites. Axtell, Kaba, Leoville, Bali, Arch and ALHA81003 are type 3.0-3.1, while ALH84018, Efremovka, Grosnaja, Allende and Vigarano are type 3.2-3.3 and Coolidge and Loongana 001 are type 3.8. Mokoia is probably a breccia with regions ranging in petrologic type from 3.0 to 3.2. Renazzo often plots at the end of the reduced and oxidized CV chondrite trends, even when those trends diverge, suggesting that in many respects it resembles the unmetamorphosed precursors of the CV chondrites. The low-petrographic types and low-TL peak temperatures of all samples, including the CV3.8 chondrites, indicates metamorphism in the stability field of low feldspar (i.e., less than 800 C) and a metamorphic history similar to that of the CO chondrites but unlike that of the ordinary chondrites.

Guimon, R. Kyle; Symes, Steven J. K.; Sears, Derek W. G.

1995-01-01

194

Determination of the petrologic type of CV3 chondrites by Raman spectroscopy of included organic matter  

NASA Astrophysics Data System (ADS)

This paper reports the first reliable quantitative determination of the thermal metamorphism grade of a series of nine CV3 chondrites: Allende, Axtell, Bali, Mokoia, Grosnaja, Efremovka, Vigarano, Leoville, and Kaba. The maturity of the organic matter in matrix, determined by Raman spectroscopy, has been used as a powerful metamorphic tracer, independent of the mineralogical context and extent of aqueous alteration. This tracer has been used along with other metamorphic tracers such as Fe zoning in type-I chondrules of olivine phenocrysts, presolar grain abundance and noble gas abundance (bulk and P3 component). The study shows that the petrologic types determined earlier by Induced ThermoLuminescence were underestimated and suggests the following values: PT (Allende-Axtell) >3.6; PT (Bali-Mokoia-Grosnaja) ˜3.6; PT (Efremovka-Leoville-Vigarano) = 3.1-3.4; PT (Kaba) ˜3.1. The most commonly studied CV3, Allende, is also the most metamorphosed. Bali is a breccia containing clasts of different petrologic types. The attribution suggested by this study is that of clasts of the highest petrologic types, as pointed out by IOM maturity and noble gas bulk abundance. CV3 chondrites have complex asteroidal backgrounds, with various degrees of aqueous alteration and/or thermal metamorphism leading to complex mineralogical and petrologic patterns. (Fe,Mg) chemical zoning in olivine phenocrysts, on the borders of type I chondrules of porphyritic olivine- and pyroxene-rich textural types, has been found to correlate with the metamorphism grade. This suggests that chemical zoning in some chondrules, often interpreted as exchanges between chondrules and nebular gas, may well have an asteroidal origin. Furthermore, the compositional range of olivine matrix is controlled both by thermal metamorphism and aqueous alteration. This does not support evidence of a nebular origin and does not necessarily mirror the metamorphism grade through (Fe,Mg) equilibration. On the other hand, it may provide clues on the degree of aqueous alteration vs. thermal metamorphism and on the timing of both processes. In particular, Mokoia experienced significant aqueous alteration after the metamorphism peak, whereas Grosnaja, which has similar metamorphism grade, did not.

Bonal, Lydie; Quirico, Eric; Bourot-Denise, Michèle; Montagnac, Gilles

2006-04-01

195

YEAST dynamics during the natural fermentation process of table olives (Negrinha de Freixo cv.).  

PubMed

Yeast population and dynamics associated to spontaneous fermentation of green table olives Negrinha de Freixo cv. were evaluated. Olives and brine samples were taken at different fermentation times, and yeast were enumerated by standard plate count and identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (rDNA). Saccharomyces cerevisiae was the most frequent, followed by Candida tropicalis, Pichia membranifaciens and Candida boidini, representing together 94.8% of the total isolates. Galactomyces reessii was also identified for the first time in table olives. The highest species diversity was found between 44 and 54 days of fermentation, both in brine and olive pulp. Furthermore, high similarity was observed between brine and olive pulp microbiotas. In conclusion, these results give valuable information to table olive industrials in order to achieve more knowledge on the fermentation process of this important Protected Designation of Origin product. PMID:25475331

Pereira, Ermelinda L; Ramalhosa, Elsa; Borges, Ana; Pereira, José A; Baptista, Paula

2015-04-01

196

Partial melting of the Allende (CV3) meteorite - Implications for origins of basaltic meteorites  

NASA Astrophysics Data System (ADS)

Eucrites and angrites are distinct types of basaltic meteorites whose origins are poorly known. Experiments in which samples of the Allende (CV3) carbonaceous chondrite were partially melted indicate that partial melts can resemble either eucrites or angrites, depending only on the oxygen fugacity. Melts are eucritic if this variable is below that of the iron-wuestite buffer or angritic if above it. With changing pressure, the graphite-oxygen redox reaction can produce oxygen fugacities that are above or below those of the iron-wuestite buffer. Therefore, a single, homogeneous, carbonaceous planetoid greater than 110 kilometers in radius could produce melts of drastically different composition, depending on the depth of melting.

Jurewicz, A. J. G.; Mittlefehldt, D. W.; Jones, J. H.

1991-05-01

197

Inducing gravitropic curvature of primary roots of Zea mays cv Ageotropic  

NASA Technical Reports Server (NTRS)

Primary roots of the mutant 'Ageotropic' cultivar of Zea mays are nonresponsive to gravity. Their root caps secrete little or no mucilage and touch the root only at the extreme apex. A gap separates the cap and root at the periphery of the cap. Applying mucilage from normal roots or substances with a consistency similar to that of mucilage to tips of mutant roots causes these roots to become strongly graviresponsive. Gravicurvature stops when these substances are removed. Caps of some mutants secrete small amounts of mucilage and are graviresponsive. These results indicate that (a) the lack of graviresponsiveness in the mutant results from disrupting the transport pathway between the cap and root, (b) movement of the growth-modifying signal from the cap to the root occurs via an apoplastic pathway, and (c) mucilage is necessary for normal communication between the root cap and root in Zea mays cv Ageotropic.

Moore, R.; Evans, M. L.; Fondren, W. M.

1990-01-01

198

Cucurbitane and hexanorcucurbitane glycosides from the fruits of Cucurbita pepo cv dayangua.  

PubMed

Phytochemical investigation of the fruits of Cucurbita pepo cv dayangua has led to the isolation of two cucurbitane glycosides: cucurbitacin L 2-O-beta-D-glucopyranoside (1), cucurbitacin K 2-O-beta-D-glucopyranoside (2) and two hexanorcucurbitane glycosides: 2,16-dihydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 2-O-beta-D-glucopyranoside (3) and 16-hydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranoside (4). Compounds 1, 2 and 3 were isolated from Cucurbita genus for the first time, while compound 4 is a new one. Their structures were determined on the basis of chemical and spectroscopic evidence. PMID:17885839

Wang, Da-Cheng; Pan, Hong-Yu; Deng, Xu-Ming; Xiang, Hua; Gao, Hui-Yuan; Cai, Hui; Wu, Li-Jun

2007-01-01

199

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering.  

PubMed

High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B. juncea cv. Geeta. Hypocotyl from 5-day-old seedlings were used as explants. Of the various combinations of auxins and cytokinins tried along with Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium, MS + IAA (0.2 mg/l) + BA (3 mg/l) proved best for shoot regeneration with 89.9 % regeneration efficiency. To induce early flowering Leafy gene from Arabidopsis thaliana was transformed using Agrobacterium mediated transformation method. After 12 weeks transgenic plants showed flowering in vitro whereas their untransformed counterpart did not flower even after 16 weeks. The maximum transformation frequency was 4 %. PMID:24431514

Sahni, Sumit; Ganie, Showkat Hussain; Narula, Alka; Srivastava, Prem Shankar; Singh, Hari Bansh

2013-07-01

200

Petrography and classification of refractory inclusions in the Allende and Mokoia CV3 chondrites  

NASA Technical Reports Server (NTRS)

Results are reported for a comprehensive petrographic survey of the refractory inclusions in the Allende and Mokoia CV3 chondrites. More than 600 refractory inclusions in 22 thin sections of the meteorites were studied by optical and scanning-electron microscopy. Olivine-rich inclusions and Ca, Al-rich inclusions (CAIs) are aggregates of various combinations of three fundamental petrographic constituents: rimmed concentric objects, Ca, Si-rich chaotic material, and mafic inclusion matrix. A new classification system for refractory inclusions is developed that is based on the size and abundance of these three fundamental constituents. The new classification system avoids several problems that are inherent in other classification systems, which use the term 'coarse-grained' too restrictively for many simple CAIs and inaccurately for most mililite-rich complex CAIs.

Kornacki, A. S.; Cohen, R. E.; Wood, J. A.

1983-01-01

201

Direct and efficient plant regeneration from leaf explants of Solanum tuberosum l. cv. Bintje.  

PubMed

A two-step procedure was used for plant regeneration from in vitro grown leaf strips (2-3 mm wide) of cv. Bintje. Step I medium was designed with 2,4-dichlorophenoxycetic acid (2,4-D) at 0.0 or 9.0 ?M, in combination with 2.28 ?M kinetin (K), benzyl adenine (BA), zeatin (Z) or zeatin riboside (ZR). Step II media were 2,4-D-free media containing 5.78 ?M gibberellic acid (GA3) and growth regulators similar to those of step I media. Leaf explants cultured in medium I containing zeatin riboside or zeatin for 6 days and then subcultured in medium II containing zeatin riboside produced numerous shoots without callus formation. Zeatin riboside containing step I and II media caused shoot regeneration in a high number (97.5±2.2) of explants. Approximately, 33.7±8.4 shoots were regenerated from each leaf explant. PMID:24194313

Yadav, N R; Sticklen, M B

1995-07-01

202

Flight-determined derivatives and dynamic characteristics of the CV-990 airplane  

NASA Technical Reports Server (NTRS)

Flight-determined longitudinal and lateral-directional stability and control derivatives are presented for the CV-990 airplane for various combinations of Mach number, altitude, and flap setting throughout the flight envelope up to a Mach number of 0.87. Also presented are the dynamic characteristics of the aircraft calculated from the flight-obtained derivatives and the measured phugoid characteristics. The derivative characteristics were obtained from flight records of longitudinal and lateral-directional transient oscillation maneuvers by using a modified Newton-Raphson digital derivative determination technique. Generally the derivatives exhibited consistent variation with lift coefficient in the low-speed data and with Mach number and altitude in the high-speed data. Many also varied with flap deflection, notably spoiler effectiveness and directional stability.

Gilyard, G. B.

1972-01-01

203

Overseas trip report, CV 990 underflight mission. [Norwegian Sea, Greenland ice sheet, and Alaska  

NASA Technical Reports Server (NTRS)

The scanning microwave radiometer-7 simulator, the ocean temperature scanner, and an imaging scatterometer/altimeter operating at 14 GHz were carried onboard the NASA CV-990 over open oceans, sea ice, and continental ice sheets to gather surface truth information. Data flights were conducted over the Norwegian Sea to map the ocean polar front south and west of Bear Island and to transect several Nimbus-7 footprints in a rectangular pattern parallel to the northern shoreline of Norway. Additional flights were conducted to obtain correlative data on the cryosphere parameters and characteristics of the Greenland ice sheet, and study the frozen lakes near Barrow. The weather conditions and flight path way points for each of the nineteen flights are presented in tables and maps.

Gloersen, P.; Crawford, J.; Hardis, L.

1980-01-01

204

Bioresorbable adhesion barrier for reducing the severity of postoperative cardiac adhesions: Focus on REPEL-CV®  

PubMed Central

Treatment of a number of congenital heart defects often necessitates staged surgical intervention. In addition, substantial improvements in postoperative cardiac care and more liberal use of biological valve substitutes have resulted in many adult patients surviving to become potential candidates for reoperations to repair or replace valves or to undergo additional revascularization procedures. In all these scenarios, surgeons are confronted with cardiac adhesions, leading to an increased surgical risk. Thus, bioresorbable adhesion barriers had become of increasing interest because they are easy to use, and safe and effective. This review focuses on the mechanisms by which REPEL-CV® prevents adhesive processes, as well as the development, design, and materials used, and also summarizes efficacy studies, clinical data, safety, and current role in therapy. PMID:22915926

Haensig, Martin; Mohr, Friedrich Wilhelm; Rastan, Ardawan Julian

2011-01-01

205

Postharvest treatments with ethylene on Vitis vinifera (cv Sangiovese) grapes affect berry metabolism and wine composition.  

PubMed

Grapes (Vitis vinifera, cv Sangiovese), harvested at standard commercial maturity, were treated for 36 h with ethylene (ET, 1000 ppm) or air (control, CT) before vinification. The composition of the grapes, must and wine was different in the CT and ET samples. In the ET wine, higher concentrations of specific phenol compounds, belonging to the classes of flavonols, anthocyanins, flavan-3-ols, and stilbenes, were detected. ET induced a significant change in the wine aroma profile by increasing free volatile categories such as phenols and fatty acids, and reducing the content of carbonyl compounds and, in particular, of esters. Less pronounced differences between CT and ET wines were observed in terms of glycosidically-bound volatile compounds. The activity of pectin methyl esterase and ?-glucosidase was enhanced in ET-treated berry skins, suggesting that cell wall properties and changes in the hydrolytic activity are effective in modulating the composition of CT and ET wines. PMID:24767053

Becatti, Elisa; Genova, Giuseppe; Ranieri, Annamaria; Tonutti, Pietro

2014-09-15

206

Effects of agricultural practices on color, carotenoids composition, and minerals contents of sweet peppers, cv. Almuden.  

PubMed

Consumers demand organic products because they believe they are more flavorful and respectful to the environment and human health. The effects of conventional, integrated, and organic farming, grown in a controlled greenhouse, on color, minerals, and carotenoids of sweet pepper fruits ( Capsicum annuum), cv. Almuden, were studied. Experimental results proved that organic farming provided peppers with the highest (a) intensities of red and yellow colors, (b) contents of minerals, and (c) total carotenoids. Integrated fruits presented intermediate values of the quality parameters under study, and conventional fruits were those with the lowest values of minerals, carotenoids, and color intensity. As an example, the concentrations of total carotenoids were 3231, 2493, and 1829 mg kg (-1) for organic, integrated, and conventional sweet peppers, respectively. Finally, organic red peppers could be considered as those having the highest antioxidant activity of all studied peppers (agricultural farming and development stage). PMID:17822289

Pérez-López, Antonio José; López-Nicolas, José Manuel; Núñez-Delicado, Estrella; Del Amor, Francisco M; Carbonell-Barrachina, Angel A

2007-10-01

207

Indicain, a dimeric serine protease from Morus indica cv. K2.  

PubMed

A high molecular mass serine protease has been purified to homogeneity from the latex of Morus indica cv. K2 by the combination of techniques of ammonium sulfate precipitation, hydrophobic interaction chromatography, and size-exclusion chromatography. The protein is a dimer with a molecular mass of 134.5 kDa and with two monomeric subunits of 67.2 kDa and 67.3 (MALDI-TOF), held by weak bonds susceptible to disruption on exposure to heat and very low pH. Isoelectric point of the enzyme is pH 4.8. The pH and temperature optima for caseinolytic activity were 8.5 and 80 degrees C, respectively. The extinction coefficient (epsilon280(1%)) of the enzyme was estimated as 41.24 and the molecular structure consists of 52 tryptophan, 198 tyrosine and 42 cysteine residues. The enzyme activity was inhibited by phenylmethylsulfonylflouride, chymostatin and mercuric chloride indicating the enzyme to be a serine protease. The enzyme is fairly stable and similar to subtilases in its stability toward pH, strong denaturants, temperature, and organic solvents. Polyclonal antibodies specific to enzyme and immunodiffusion studies reveal that the enzyme has unique antigenic determinants. The enzyme has activity towards broad range of substrates comparable to those of subtilisin like proteases. The N-terminal residues of indicain (T-T-N-S-W-D-F-I-G-F-P) exhibited considerable similarity to those of other known plant subtilases, especially with cucumisin, a well-characterized plant subtilase. This is the first report of purification and characterization of a subtilisin like dimeric serine protease from the latex of M. indica cv. K2. Owing to these unique properties the reported enzyme would find applications in food and pharma industry. PMID:18561962

Singh, Vijay Kumar; Patel, Ashok Kumar; Moir, A J; Jagannadham, Medicherla V

2008-08-01

208

Quantifying key parameters as elicitors for alternate fruit bearing in cv. 'Elstar' apple trees.  

PubMed

The commonly known alternate bearing, i.e. year-to-year change of large and small yields of fruit tree crops worldwide, is often induced by abiotic stress such as late frost, which will eliminate flowers or fruitlets. This study presents an alternative form, biotic biennial bearing, i.e. change of large and small yields of the same trees within the same tree row in the same year. Three methods were developed or modified for the analysis of the number of flower clusters and yield of 2086 apple (Malus domestica Borkh.) cv. 'Elstar' trees. The first method, i.e., based on intersect between yield in year x and year x+1 and flower clusters in year x, yielded 91-106 flower clusters, whereas the second method, i.e., mean yield in year x and year x+1, resulted in a range of 72-133 flower clusters, or 9.6kg/tree necessary for sustainable cultivation of apple cv. 'Elstar'. The third 'biennial bearing index' (BBI), was calculated in three ways as the ratio of differences in tree yields to cumulative tree yield, for individual trees (rather than orchard average) to demonstrate the tree-to-tree alternation. A scheme for the possible underlying regulatory mechanisms was developed, which includes potential elicitors such as light deprivation and subsequent lack of flower initiation, are discussed as a possible result of polar basipetal GA7 transport, cytokinin level in the xylem and phloem and down-regulation of the gene expression of the flowering gene. Suggested countermeasures included early chemical or mechanical thinning. PMID:24094049

Krasniqi, Anne-Lena; Damerow, Lutz; Kunz, Achim; Blanke, Michael M

2013-11-01

209

Economic valuation of the Seto Inland Sea by using an Internet CV survey.  

PubMed

We estimate the economic value of the natural environment damaged in the Seto Inland Sea after the introduction of the Law on Temporary Measures for the Environmental Conservation of the Seto Inland Sea (Setouchi Law) and the value of the natural environment that survived, using a Contingent Valuation (CV) survey on an Internet web site. The CV survey contains three plans. Plan 1 is to restore 4 ha of reclaimed land. By estimating the Willingness To Pay (WTP) for plan 1, we can appraise the value of the natural environment that was damaged as a result of the original reclamation. Plan 2 is to transplant Zostera (eel-grass) into an area of 10 ha offshore. Plan 3 is to preserve the shore area, a natural habitat for rare animal species, under the National Trust Program. From the WTP for plans 2 and 3, we can estimate the value of the shore area and the areas a little farther offshore. The value of the natural environment damaged in the Seto Inland Sea as a result of reclaiming projects after the introduction of the Setouchi Law and the value of the existing natural environment of the Seto Inland Sea from the WTP for the plans were estimated to about 172 trillion yen (1.46 trillion dollars) and about 424 trillion yen (3.60 trillion dollars), respectively. The results indicate that in the 25 years since the introduction of the Setouchi Law, we have degraded every year about 6.88 trillion yen (58.5 billion dollars) worth of the natural environment by reclaiming. Some seaweed farms and natural shore areas, natural habitats to rare marine life-forms like the horseshoe crab and the fiddler crab have survived, but their value amounts to about 80% of Japan's GDP. PMID:12787625

Tsuge, Takahiro; Washida, Toyoaki

2003-01-01

210

Synchronizing the Absolute and Relative Clocks: Pb-Pb and Al-Mg Systematics in CAIs from the Allende and NWA 2364 CV3 Chondrites  

NASA Astrophysics Data System (ADS)

A Pb-Pb internal isochron of a type-B CAI from the NWA 2364 CV3 chondrite gives an absolute age of 4568.6 ± 0.2 Ma which contrasts with previous internal Pb-Pb ages of CAIs from Allende and Efremovka. Al-Mg systematics are also reported for CV3 CAIs.

Bouvier, A.; Wadhwa, M.

2009-03-01

211

Toward positional cloning of everblooming gene (evb) in plants: a BAC library of Rosa chinensis cv. old blush  

E-print Network

megabase-size DNA was isolated from the recurrent blooming diploid species, Rosa chinensis cv. Old Blush. The DNA was then partially digested with BamHI and separated on agarose gels by multi-phase pulsed-field gel electrophoresis. Size selected fragments...

Hess, Gregory

2006-10-30

212

First report of downy mildew caused by Plasmopara halstedii on black-eyed susan (Rudbeckia fulgida cv. ‘Goldsturm’) in Maryland  

Technology Transfer Automated Retrieval System (TEKTRAN)

The North American perennial black-eyed Susan (Rudbeckia fulgida cv. ‘Goldsturm’) is an important nursery crop, prized by gardeners and landscapers for its persistent bloom and ease of cultivation. In September 2013 disease symptoms characteristic of downy mildew were observed from multiple plants a...

213

Extraction of arbutin and its comparative content in branches, leaves, stems, and fruits of Japanese pear Pyrus pyrifolia cv. Kousui.  

PubMed

Arbutin is a tyrosinase inhibitor and is extensively used as a human skin-whitening agent. This study investigated the optimum conditions for extracting arbutin by ultrasonic homogenization from discarded branches pruned from Japanese pear (Pyrus pyrifolia cv. Kousui) trees. The arbutin content was measured in the branches and also in the leaves, stems, fruit peel, and fruit flesh. PMID:25035992

Sasaki, Chizuru; Ichitani, Masaki; Kunimoto, Ko-Ki; Asada, Chikako; Nakamura, Yoshitoshi

2014-01-01

214

Are the input parameters of white noise driven integrate and fire neurons uniquely determined by rate and CV?  

E-print Network

by rate and CV? Rafael D. Vilela �, Benjamin Lindner Max-Planck-Institut fu¨r Physik komplexer Systeme, No IF models have been proposed, the firing statistics of which depends nontrivially on the input parameters m is associated with a given set of basic firing statistics as, for instance, the firing rate and the coefficient

Lindner, Benjamin

215

Are the input parameters of white noise driven integrate and fire neurons uniquely determined by rate and CV?  

PubMed

Integrate and fire (IF) neurons have found widespread applications in computational neuroscience. Particularly important are stochastic versions of these models where the driving consists of a synaptic input modeled as white Gaussian noise with mean mu and noise intensity D. Different IF models have been proposed, the firing statistics of which depends nontrivially on the input parameters mu and D. In order to compare these models among each other, one must first specify the correspondence between their parameters. This can be done by determining which set of parameters (mu,D) of each model is associated with a given set of basic firing statistics as, for instance, the firing rate and the coefficient of variation (CV) of the interspike interval (ISI). However, it is not clear a priori whether for a given firing rate and CV there is only one unique choice of input parameters for each model. Here we review the dependence of rate and CV on input parameters for the perfect, leaky, and quadratic IF neuron models and show analytically that indeed in these three models the firing rate and the CV uniquely determine the input parameters. PMID:19063904

Vilela, Rafael D; Lindner, Benjamin

2009-03-01

216

Wintercuring of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid in response to Xylella fastidiosa infections  

Technology Transfer Automated Retrieval System (TEKTRAN)

Clonal replicates of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid P 63-61 were inoculated with Xylella fastidiosa strain M23 and evaluated for Almond Leaf Scorch Disease and subsequent wintercuring of infections during three growing seasons. Initial inoculations established gr...

217

Water Deficit Effect on Ratio of Seed to Berry Fresh Weight and Berry Weight Uniformity in Winegrape cv. Merlot  

Technology Transfer Automated Retrieval System (TEKTRAN)

Field-grown grapevines cv. Merlot (Vitis vinifera L.) were differentially irrigated in a randomized block design during two growing seasons to maintain a high or low level of vine water stress between fruit set and harvest. Detached berries from clusters harvested at maturity were individually weig...

218

Effect of Cutting Heights on Productivity and Quality of King Napier Grass (Pennisetum purpureum cv. King Grass) under Irrigation  

Microsoft Academic Search

2 Abstract: An experiment to study the effect of cutting heights on yield and nutritive values of King napier grass (Pennisetum purpureum cv. King grass) was conducted on sandy loam, Korat soil series (Oxic Paleustults) under irrigation during June 2006 to November 2007 at Khon Kaen Animal Nutrition Development Research Center, Thailand. Four treatments of cutting height at 0 (T1),

Sumran Wijitphan; Pornchai Lorwilai; Chutipong Arkaseang

2009-01-01

219

Vis\\/NIR soluble solids prediction in intact oranges ( Citrus sinensis L.) cv. Valencia Late by reflectance  

Microsoft Academic Search

A commercially available instrument of near infrared spectroscopy, equipped with a post-dispersive reflectance configuration, was used for the development of partial least squares calibration models for soluble solids content and total acidity prediction in oranges (Citrus sinensis L.) cv. Valencia Late. Both internal validation and external validation were conducted and modified partial least squares regression was also tested. The best

J. A. Cayuela

2008-01-01

220

A hierarchical neural network model based on a C\\/V segmentation algorithm for isolated Mandarin speech recognition  

Microsoft Academic Search

A novel algorithm simultaneously performing consonant\\/vowel (C\\/V) segmentation and pitch detection is proposed. Based on this algorithm, a consonant enhancement method and a hierarchical neural network scheme are explored for Mandarin speech recognition. As a result, an improvement of 12% in consonant recognition rate is obtained and the number of recognition candidates is reduced from 1300 to 63. A series

Jhing-Fa Wang; Chung-Hsien Wu; Shih-Hung Chang; Jau-Yien Lee Lee

1991-01-01

221

High Efficiency Somatic Embrogenesis and Plant Regeneration in Suspension Cultures of an Ornamental Ginger Hybrid (Hedychium muluense x cv ‘Starburst’)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Plants were successfully regenerated via somatic embryogenesis from shoot apex-derived callus of an ornamental ginger hybrid, Hedychium muluense x cv ‘Starburst’. H. muluense is a dwarf species and ‘Starburst’ is a hybrid cultivar with white and very fragrant flowers in a circular, wheel-like arrang...

222

Confirmation By QTL mapping Of The Malus Robusta (Cv. Robusta 5) derived powdery mildew resistance gene Pl1  

Technology Transfer Automated Retrieval System (TEKTRAN)

The Geneva® apple rootstock breeding program has made extensive use of Malus robusta cv. Robusta 5 as a source of resistance to fire blight. Robusta 5 has also been used as the source of powdery mildew resistance by other breeding programs and a single locus Pl1 has been associated with this resist...

223

Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg  

E-print Network

Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg GRECO Cvo UNI OL/ Nds.PV-Symp. 06-2009 semiconductor physics / quantum solar energy conversion;Semiconductor Physics and Quantum Solar Energy Conversion Semiconductor Physics and Quantum Solar Energy

224

Influência de fatores químicos do solo sobre a incidência do mal-do-Panamá na bananeira cv. pacovan na Paraíba  

Microsoft Academic Search

This objective of this work was to investigate the correlation of chemicals soil factors and its influences with the incidence and severity of Panama disease ( Fusarium oxysporum f. sp. cubense ) in banana tree ( Musa sapientium cv. pacovan) in the State of Paraíba. The work was carried out at Areia, Alagoa Nova, Bananeiras and Lagoa Seca Municipalities, where

Edson Batista Lopes; Carlos Henrique de Brito; Ivanildo Cavalcanti de Albuquerque; Arlington Ricardo

225

The influence of fruit ripening on the phytochemical content and biological activity of Capsicum chinense Jacq. cv Habanero  

Microsoft Academic Search

During the past decade, it has been reported that the consumption of certain foods and spices such as pepper may have a positive effect on health. The present study evaluates the influence of fruit ripening on total phenols, flavonoids, carotenoids and capsaicinoids content and antioxidant, hypoglycaemic and anticholinesterase activities of Capsicum chinense Jacq. cv Habanero. The chemical investigation showed a

Federica Menichini; Rosa Tundis; Marco Bonesi; Monica R. Loizzo; Filomena Conforti; Giancarlo Statti; Bruno De Cindio; Peter J. Houghton; Francesco Menichini

2009-01-01

226

Internship Checklist for DEB students 1. Before you submit a CV and cover letter to any company, please have Dr.  

E-print Network

Internship Checklist for DEB students Feb 2008 1. Before you submit a CV and cover letter to any an internship (in Objective section) and also list the DEB as part of your PhD education. Example: PhD in Chemistry with a Designated Emphasis in Biotechnology. 3. If you are offered an internship, please notify Dr

Ferrara, Katherine W.

227

Inherited transgene expression of the uidA and bar genes in Lilium longiflorum cv. Nellie White  

Technology Transfer Automated Retrieval System (TEKTRAN)

The expression of two transgenes, bar and uidA, was studied in Lilium longiflorum cv. Nellie White plants. ‘Nellie White’ had been transformed using the gene gun to bombard with pDM327 that contains the bar-uidA fusion gene under control of the CaMV 35S promoter. PCR analysis confirmed that eight ...

228

Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)  

Microsoft Academic Search

Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the

D. V. Lynch; P. L. Steponkus

1987-01-01

229

The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface.  

PubMed

The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is "hijacked" by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 "self"-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A; Bianchet, Mario A; Wang, Lai-Xi; Wilson, Iain B H; Vasta, Gerardo R

2013-08-23

230

The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface*  

PubMed Central

The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is “hijacked” by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288,) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 “self”-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N.; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A.; Bianchet, Mario A.; Wang, Lai-Xi; Wilson, Iain B. H.; Vasta, Gerardo R.

2013-01-01

231

Coventry University College is a trading name of Coventry University College Limited. A company registered in England and Wales under company number 07781274. Registered office: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB  

E-print Network

Street, Coventry, United Kingdom CV1 5FB JOB DESCRIPTION & PERSON SPECIFICATION 1. JOB INFORMATION Post. Registered office: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB 10. To undertake Street, Coventry, United Kingdom CV1 5FB 3. PERSON SPECIFICATION This is a new and exciting role in a new

Low, Robert

232

CvL, a lectin from the marine sponge Cliona varians: Isolation, characterization and its effects on pathogenic bacteria and Leishmania promastigotes.  

PubMed

CvL, a lectin from the marine sponge Cliona varians was purified by acetone fractionation followed by Sepharose CL 4B affinity chromatography. CvL agglutinated papainized treated human erythrocytes with preference for type A erythrocytes. The lectin was strongly inhibited by monosaccharide d-galactose and disaccharide sucrose. CvL is a tetrameric glycoprotein of 28 kDa subunits linked by disulphide bridges with a molecular mass of 106 kDa by SDS-PAGE and 114 kDa by Sephacryl S300 gel filtration. The lectin was Ca2+ dependent, stable up to 60 degrees C for 60 min, with optimum pH of 7.5. CvL displays a cytotoxic effect on gram positive bacteria, such as Bacillus subtilis and Staphylococcus aureus. However, CvL did not affect gram negative bacteria, such as Escherichia coli and Pseudomonas aeruginosa. Leishmania chagasi promastigotes were agglutinated by CvL up to 2(8) titer. These findings are indicative of the physiological defense roles of CvL and its possible use in the antibiosis of bacteria and protozoa pathogenic. PMID:17020812

Moura, Raniere M; Queiroz, Alexandre F S; Fook, Jacy M S L L; Dias, Anny S F; Monteiro, Norberto K V; Ribeiro, Jannisson K C; Moura, Gioconda E D D; Macedo, Leonardo L P; Santos, Elizeu A; Sales, Maurício P

2006-12-01

233

Concentration and pump intensity dependent gain studies for disodium flourescein (FDS), cresyl violet (CV), and rhodamine- 590 (Cl) and cresyl violet mixture  

NASA Astrophysics Data System (ADS)

The dye concentration dependence gain spectra for disodium fluorescein (FDS) cresyl violet (CV) and a dye mixture [Rh 590 (Cl)+CV] dissolved in methanol have been studied by amplified spontaneous emission (ASE) technique under pulsed N 2-laser excitation. On comparing the efficiency curves, the dominant role of photoquenching (excited state absorption) was clearly observed in the non-rigid dye FDS, whereas it is non-existent in rigid dye CV at the same concentration. In case of a dye mixture, the energy transfer excitation was found to overcome the inner filter effect and other losses. The various energy transfer mechanisms in these dyes have also been discussed.

Gaur, Arun; Taneja, L.; Sharma, A. K.; Mohan, D.; Singh, R. D.

1991-06-01

234

Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. Cv. MRQ 74)  

PubMed Central

Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5?mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5?mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1?mg/L BAP, and 0.1?mg/L ?-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1?mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds. PMID:22919338

Taha, Rosna Mat; Saleh, Azani; Mahmad, Noraini; Hasbullah, Nor Azlina; Mohajer, Sadegh

2012-01-01

235

Brassinosteroid stimulation of hypocotyl elongation and wall relaxation in pakchoi (Brassica chinensis cv Lei-Choi)  

SciTech Connect

Hypocotyl elongation of pakchoi (Brassica chinensis cv Lei-Choi) was stimulated by applying 300 ng of brassinosteroid (2[alpha],3[alpha],22[beta],23[beta]-tetrahydroxy-24[beta]-methyl-B-homo-7-oxa-5[alpha]-cholestan-6-one, BR) in 1 [mu]L of 50% ethanol to the apex of hypocotyls. BR had its greatest effect on elongation of the apical 3-mm region below the cotyledonary node (75% stimulation) between 6 and 18 h after treatment. Stress/strain (Instron) analysis of this 3-mm region revealed that plastic and elastic components of extension were not significantly different between BR-treated and control seedlings. In pressure-block experiments, the initial rate of relaxation was 2-fold faster in BR-treated plants as compared with controls, whereas after 125 min the total amount of relaxation and the relaxation rate were the same for the two treatments. Osmotic pressure of cell sap expressed from this 3-mm region showed a large decrease (28%) in BR-treated seedlings compared to the controls. The authors conclude that BR stimulates growth in pakchoi by accelerating the biochemical processes that cause wall relaxation, without inducing a large change in wall mechanical properties. 19 refs., 3 figs., 1 tab.

Tzannwei Wang; Cosgrove, D.J.; Arteca, R.N. (Pennsylvania State Univ., University Park (United States))

1993-03-01

236

Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers  

SciTech Connect

The transport properties of Ca-pumping ATPases from carrot (Daucus carota cv. Danvers) tissue culture cells were studied. ATP dependent Ca transport in vesicles that comigrated with an ER marker, was stimulated 3-4 fold by calmodulin. Cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum Ca-ATPase) partially inhibited oxalate-stimulated Ca transport activity; however, it had little or not effect on calmodulin-stimulated Ca uptake. The results suggested the presence of two types of Ca ATPases, and ER- and a plasma membrane-type. Incubation of membranes with (gamma{sup 32}P)ATP resulted in the formation of a single acyl ({sup 32}P) phosphoprotein of 120 kDa. Formation of this phosphoprotein was dependent on Ca, and enhanced by La {sup 3+}, characteristic of the plasma membrane CaATPase. Acidic phospholipids, like phosphatidylserine, stimulated Ca transport, similar to their effect on the erythrocyte plasma membrane CaATPase. These results would indicate that the calmodulin-stimulated Ca transport originated in large part from a plasma membrane-type Ca pump of 120 kDa.

Wenling Hsieh; Sze, Heven (Univ. of Maryland, College Park (United States))

1991-05-01

237

Changes in phenol content during strawberry (Fragariaxananassa, cv. Chandler) callus culture.  

PubMed

Total soluble phenols, soluble flavanols, (+)-catechin, ferulic acid and 1-O-feruloyl-beta-d-glucose were analyzed during the development of a strawberry (Fragariaxananassa, cv. Chandler) callus culture. The time-course changes of the different phenols assayed were well correlated with callus growth and morphology. The changes in polyphenol oxidase (EC 1.10.3.1-2) and beta-glucosidase (EC 3.2.1.21) activities in the callus were also examined. The total phenol, soluble flavanols and (+)-catechin contents were high during the preexponential and exponential phases of growth. The subsequent decrease in (+)-catechin concentration coincided with high levels of polyphenol oxidase activity. The 1-O-feruloyl-beta-d-glucose content was highest as callus growth ceased, and its subsequent decrease was accompanied by the increased production of ferulic acid. This increase in ferulic acid was accompanied by an increase in beta-glucosidase activity. The ferulic acid content decreased at the end of culture, when callus growth had stopped and showed clear symptoms of senescence. This decrease in the ferulic acid concentration was accompanied by an increase in the levels of ferulic acid bound to cell wall components. PMID:12060275

López Arnaldos, Tomás; Muñoz, Romualdo; Ferrer, María Angeles; Calderón, Antonio A.

2001-11-01

238

Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex  

PubMed Central

Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees’ latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins’ autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458

Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

2013-01-01

239

Chemical and spectroscopic characteristics of the wood of Vitis vinifera cv. Sangiovese affected by esca disease.  

PubMed

Chemical and spectroscopic analyses ((13)C cross-polarization-magic angle spinning NMR and attenuated total reflection Fourier transform infrared spectroscopies) were carried out on the wood of Vitis vinifera cv. Sangiovese with brown-red discoloration and black streaks caused by esca disease. The analyses of the brown-red wood revealed the destruction of hemicelluloses and noncrystalline cellulose as well as modifications in the pectic and ligninic wood fractions. The pectic fraction consisted of carbohydrates associated with polyphenols. The lignin fraction exhibited only a few changes in the aromatic systems and a partial demethylation, and it appeared to be associated with condensed phenolic components probably arising from response polyphenols. The degradation of hemicelluloses and noncrystalline cellulose in brown-red wood, where the pathogens Phaeoacremonium aleophilum and Phaeomoniella chlamydospora prevail with respect to the other fungus Fomitiporia mediterranea, was consistent with reports on the degradative activity of such fungi in vitro carried out on model substrates. The observed alterations could also be attributed to the radical oxidation process caused by the oxidative response of defense itself triggered by infection, as suggested by the accumulation of postinfectional compounds. The analyses of wood tissue with black streaks showed less marked deterioration; here, an increase in pectic and phenolic substances, which probably accumulate in the xylem vessels as a response to the infection, was observed. PMID:19919033

Agrelli, Diana; Amalfitano, Carmine; Conte, Pellegrino; Mugnai, Laura

2009-12-23

240

Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex.  

PubMed

Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees' latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins' autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458

Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

2013-03-01

241

Regulation of Cytokinin Oxidase Activity in Callus Tissues of Phaseolus vulgaris L. cv Great Northern 1  

PubMed Central

The regulation of cytokinin oxidase activity in callus tissues of Phaseolus vulgaris L. cv Great Northern has been examined using an assay based on the oxidation of N6-(?2-isopentenyl)adenine-8-14C (i6 Ade-8-14C) to adenine. Solutions of exogenous cytokinins applied directly to the surface of the callus tissues induced relatively rapid increases in cytokinin oxidase activity. The increase in activity was detectable after 1 hour and continued for about 8 hours, reaching values two- to three-fold higher than the controls. The cytokinin-induced increase in cytokinin oxidase activity was inhibited in tissues pretreated with cordycepin or cycloheximide, suggesting that RNA and protein synthesis may be required for the response. Rifampicin and chloramphenicol, at concentrations that inhibited the growth of Great Northern callus tissues, were ineffective in inhibiting the increase in activity. All cytokinin-active compounds tested, including both substrates and nonsubstrates of cytokinin oxidase, were effective in inducing elevated levels of the enzyme in Great Northern callus tissue. The cytokinin-active urea derivative, Thidiazuron, was as effective as any adenine derivative in inducing this response. The addition of Thidiazuron to the reaction volumes used to assay cytokinin oxidase activity resulted in a marked inhibition of the degradation of the labeled i6 Ade-8-14C substrate. On the basis of this result, it is possible that Thidiazuron may serve as a substrate for cytokinin oxidase, but other mechanisms of inhibition have not yet been excluded. PMID:16664650

Chatfield, J. Mark; Armstrong, Donald J.

1986-01-01

242

Visualisation and quantification of CV chondrite petrography using micro-tomography  

NASA Astrophysics Data System (ADS)

Micro-computed tomography is a non-destructive technique that allows the study of 3D meteorite petrography. The technique produces a unique and instructive visualisation of the meteorite for quantifying its components. We studied the overall petrography of the two CV chondrites Allende and Mokoia to constrain their formation histories. A set of movies and stereographic images detail the 3D petrography. Component modal abundances agree with previous reports and modal abundance differences between Allende and Mokoia support the chondrule-matrix complementarity and that chondrules and matrix formed from the same chemical reservoir. We identified two types of chondrules, a normal type and one where a normal type I or II chondrule is almost completely encapsulated by an opaque-rich layer. This layer was probably acquired during a late stage condensation process. The appearance of opaques in chondrules and matrix is different, not supporting a genetic relationships between these. Low abundances of compound chondrules (1.75 vol% in Allende and 2.50 vol% in Mokoia) indicate low chondrule densities and/or low relative component velocities in chondrule formation regions. Porosities on a scale <10-20 ?m allowed for only local aqueous alteration processes on the meteorite parent bodies.

Hezel, Dominik C.; Elangovan, Premkumar; Viehmann, Sebastian; Howard, Lauren; Abel, Richard L.; Armstrong, Robin

2013-09-01

243

Chelator-enhanced lead accumulation in Agropyron elongatum cv Szarvasi-1 in hydroponic culture.  

PubMed

Hydroponic culture was applied to compare the efficiency of K2EDTA and citrate in mobilizing Pb for accumulation in Agropyron elongatum cv. Szarvasi-1 and their effects on some physiological characteristics of the plants. The plants were grown in nutrient solutions containing 0, 10, and 100 microM Pb(NO3)2 combined with chelating agents added to the nutrient solutions after 21 days of growth, in 3 concentrations (0, 100, and 500 microM). The effects were measured after eight days. The energy grass proved to be greatly resistant to the treatments, as was reflected in the slight inhibition of growth, the resistance of the photosynthetic electron transport chain and the chlorophyll composition and the lack of change in the malone-dialdehyde content. Fundamental differences can be identified between the effects of EDTA and citrate. Citrate had only a little effect on the physiological parameters, which may be due to the strongly increasing lead accumulation with increasing concentration of Pb in the nutrient solution. Additionally, citrate ensured a higher biomass yield with higher shoot Pb accumulation compared to EDTA in almost all treatments. Concerning biomass reduction, 10 microM Pb applied together with K2EDTA has the most deleterious effects on energy grass. The effects correlated with the concentration of EDTA. PMID:21598794

Vashegyi, Ildikó; Cseh, Edit; Lévai, László; Fodor, Ferenc

2011-03-01

244

Occurrence of metaxenia and false hybrids in Brassica juncea L. cv. Kikarashina × B. napus  

PubMed Central

Imported genetically modified (GM) canola (Brassica napus) is approved by Japanese law. Some GM canola varieties have been found around importation sites, and there is public concern that these may have any harmful effects on related species such as reduction of wild relatives. Because B. juncea is distributed throughout Japan and is known to be high crossability with B. napus, it is assumed to be a recipient of B. napus. However, there are few reports for introgression of cross-combination in B. juncea × B. napus. To assess crossability, we artificially pollinated B. juncea with B. napus. After harvesting a large number of progeny seeds, we observed false hybrids and metaxenia of seed coats. Seed coat color was classified into four categories and false hybrids were confirmed by morphological characteristics and random amplified polymorphic DNA (RAPD) markers. Furthermore, the occurrence of false hybrids was affected by varietal differences in B. napus, whereas that of metaxenia was related to hybridity. Therefore, we suggest that metaxenia can be used as a marker for hybrid identification in B. juncea L. cv. Kikarashina × B. napus. Our results suggest that hybrid productivity in B. juncea × B. napus should not be evaluated by only seed productivity, crossability ought to be assessed the detection of true hybrids. PMID:23136472

Tsuda, Mai; Konagaya, Ken-ichi; Okuzaki, Ayako; Kaneko, Yukio; Tabei, Yutaka

2011-01-01

245

The impact of high temperatures on Vitis vinifera cv. Semillon grapevine performance and berry ripening  

PubMed Central

The heat event that occurred in many parts of Australia in 2009 was the worst on record for the past decade, with air temperatures exceeding 40°C for 14 days. Our aim was to assess the impacts of this heat event on vine performance, including ripening, yield, and gas exchange of Vitis vinifera cv. Semillon grown in a Riverina vineyard. To assess the affect of high temperatures on Semillon grapevines, the vines were covered with a protective layer to reduce radiant heating and were compared with vines exposed to ambient conditions. The heat event had major effects on ripening; reducing the rate of ripening by 50% and delaying harvest ripeness and causing a high incidence of berry shrivel and sunburn. Yield was not affected. Photosynthesis was reduced 35% by the heat event while transpiration increased nearly threefold and was accounted for by increased stomatal conductance. The conclusion of this study was that heat events delayed ripening in Semillon berries and caused a significant reduction in berry quality. Strategies to minimize the radiant load during heat events are required and this study has confirmed a protective layer can reduce canopy temperatures and enhance berry quality. PMID:24348494

Greer, Dennis H.; Weedon, Mark M.

2013-01-01

246

In vitro regeneration in olive (Olea europaea L.) cv, 'Frontio' from nodal segments.  

PubMed

An efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv 'Frontio' has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurine (BAP), which resulted in 56.2% of bud break and 93.7% survival whereas, a combination of full strength MS medium with 1.0 mg/L each of 3-indole-butyric-acid (IBA) and kinetin was found to be the best for shoot multiplication, in terms of number of shoots (3.6 shoots/explant) and shoot length (2.2 cm). The in vitro shoots were rooted on half-strength MS medium fortified with 0.2 mg/L IBA and 0.2 mg/L alpha-naphthalene acetic acid (NAA) with 1.5 g/L activated charcoal, which supported optimum rooting (60%), with an average of 2-3 roots/shoot, about 2.4 cm length were produced on four weeks of culture. PMID:25241592

Mangal, Manisha; Sharma, Dheeraj; Sharma, Mamta; Kumar, Sunil

2014-09-01

247

Effect of sowing dates and vernalization on Betavulgaris L. cv. Univers C-leaf structure  

PubMed Central

This research was conducted to study the effect of three different sowing dates (15th October, 15th November and 15th December) and two vernalization treatments (5 °C and ?20 °C) on leaf structure of Betavulgaris L. cv. Univers. The obtained data are summarized as follows: The maximum values of the most studied parameters; lower epidermis + spongy tissue thickness, midrib, mesophyll tissue, vascular bundle, collenchymatous tissue and number of xylem vessels per arm were found as a result of 15th October sowing date treatment compared with the two other sowing dates. Furthermore, effect of the cooling treatments varied according to the recorded character, sowing date and cooling degree. Most of the vernalization treatments at early sowing dates increased the mesophyll tissue, midrib, number of vascular bundles per transverse section, vascular bundle thickness and number of xylem arms per transverse section. The two studied cooling treatments at 15th October sowing date increased both stomatal index and average number of stomata: average number of epidermis cells compared with the control. Furthermore, 15th October under ?20 °C treatment led to small epidermal cells and stomata formation, straight epidermal cell walls and closed stomata in comparison to the control. PMID:23961134

Sakr, Mohammed M.; Almaghrabi, Omar A.

2011-01-01

248

Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)  

PubMed Central

A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 ?M, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 ?M. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172

Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng

2009-01-01

249

Promotion of Flowering in Brassica campestris L. cv Ceres by Sucrose  

PubMed Central

Flower initiation of the quantitative long-day plant Brassica campestris cv Ceres was earlier and at a lower final leaf number when sucrose was added to the medium in which plants were grown in sterile culture. The optimal concentration of sucrose was 40 to 80 millimolar. This flower-promoting effect of sucrose was not osmotic, as mannitol, sodium chloride, and polyethylene glycol were not effective at equal osmotic potentials. Seedlings grown heterotrophically after treatment with 4-chloro-5-(dimethylamino)-2-phenyl-3-(2H)-pyridazinone to prevent chlorophyll accumulation were also induced to form flower primordia earlier as the sucrose concentration in the medium was increased up to 80 millimolar. Inclusion of 4 millimolar sodium nitrate in the culture medium of green plants did not reduce the flower-promoting effects of sucrose but delayed initiation in plants grown without added sucrose. Removal of CO2 during a single main or supplementary light period, or both, greatly reduced flower initiation. It is concluded that sucrose may be an important controlling factor determining floral initiation in Brassica. PMID:16663739

Friend, Douglas J. C.; Bodson, Monique; Bernier, Georges

1984-01-01

250

Perianth bottom-specific blue color development in Tulip cv. Murasakizuisho requires ferric ions.  

PubMed

The entire flower of Tulipa gesneriana cv. Murasakizuisho is purple, except the bottom, which is blue. To elucidate the mechanism of the different color development in the same petal, we prepared protoplasts from the purple and blue epidermal regions and measured the flavonoid composition by HPLC, the vacuolar pH by a proton-selective microelectrode, and element contents by the inductively coupled plasma (ICP) method. Chemical analyses revealed that the anthocyanin and flavonol compositions in both purple and blue colored protoplasts were the same; delphinidin 3-O-rutinoside (1) and major three flavonol glycosides, manghaslin (2), rutin (3) and mauritianin (4). The vacuolar pH values of the purple and blue protoplasts were 5.5 and 5.6, respectively, without any significant difference. However, the Fe(3+) content in the blue protoplast was approximately 9.5 mM, which was 25 times higher than that in the purple protoplasts. We could reproduce the purple solution by mixing 1 with two equimolar concentrations of flavonol with lambda(vismax) = 539 nm, which was identical to that of the purple protoplasts. Furthermore, addition of Fe(3+) to the mixture of 1-4 gave the blue solution with lambda(vismax) = 615 nm identical to that of the blue protoplasts. We have established that Fe(3+) is essential for blue color development in the tulip. PMID:17179184

Shoji, Kazuaki; Miki, Naoko; Nakajima, Noriyuki; Momonoi, Kazumi; Kato, Chiharu; Yoshida, Kumi

2007-02-01

251

Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.  

PubMed

Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome. PMID:19005777

Lu, Xu; Zhou, Wei; Gao, Feng

2009-09-01

252

Case study of chondrule alteration with IR spectroscopy in NWA 2086 CV3 meteorite  

NASA Astrophysics Data System (ADS)

Analyzing the alteration in an olivine chondrule of the NWA 2086 CV3 meteorite, infrared spectral, electron microprobe and optical microscopic observations were correlated to each other. The intensity and wavelength positions of olivine peaks changed characteristically with the progression of alteration and related Fe/Mg substitution inward of the chondrule. Moderate to good correlations were identified between Fo% composition and positions of 830 and 860 cm-1 IR peaks. The disappearance of 1020 cm-1 peak by structural change happens already at a low level alteration without changing the optical appearance of the mineral. The existence of the 980 cm-1 peak is found to be an indicator of the intact phase of olivine. While profiles perpendicular to the chondrule's perimeter showed that the alteration progressed 15-20 ?m distance inward without observable fractures (probablly by some diffusion related process), the "alteration distance" from various obvious fractures inside the chondrule was only 3-5 ?m distance. These observations suggest that the substitution was more effective close to the matrix, and also related to some fluids that although were able to circulate along the large internal fractures too, did not produce such strong substitution there, like what happened close to the matrix. It was also demonstrated that the poorly exploited contact mode observations with ATR based reflection method in infrared spectroscopy provide a useful tool to analyze the alteration at micrometer scale without much sample preparation, and enable identifying alterations already at such a low level where the olivines still look optically intact.

Kereszturi, A.; Gyollai, I.; Szabó, M.

2015-02-01

253

Air-sampling inlet contamination by aircraft emissions on the NASA CV-990 aircraft  

NASA Technical Reports Server (NTRS)

Results of an experimental investigation of the contamination of air sampling inlets by aircraft emissions from the NASA CV-990 research aircraft are presented. This four-engine jet aircraft is a NASA facility used for many different atmospheric and meteorological experiments, as well as for developing spacecraft instrumentation for remote measurements. Our investigations were performed to provide information on which to base the selection of sampling locations for a series of multi-instrument missions for measuring tropospheric trace gases. The major source of contamination is the exhaust from the jet engines, which generate many of the same gases that are of interest in atmospheric chemistry, as well as other gases that may interfere with sampling measurements. The engine exhaust contains these gases in mixing ratios many orders of magnitude greater than those that occur in the clean atmosphere which the missions seek to quantify. Pressurized samples of air were collected simultaneously from a scoop located forward of the engines to represent clean air and from other multiport scoops at various aft positions on the aircraft. The air samples were analyzed in the laboratory by gas chromatography for carbon monoxide, an abundant combustion by-product. Data are presented for various scoop locations under various flight conditions.

Condon, E. P.; Vedder, J. F.

1984-01-01

254

An Efficient Agrobacterium-Mediated Transformation of Strawberry cv. Camarosa by a Dual Plasmid System.  

PubMed

An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100%) of shoot formation and the highest mean number of shoots (24) produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86%) in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet. PMID:25711423

Haddadi, Fatemeh; Aziz, Maheran Abd; Abdullah, Siti Nor Akmar; Tan, Soon Guan; Kamaladini, Hossein

2015-01-01

255

CV-990 Landing Systems Research Aircraft (LSRA) flight #145 drilling of shuttle tire using Tire Assa  

NASA Technical Reports Server (NTRS)

Created from a 1/16th model of a German World War II tank, the TAV (Tire Assault Vehicle) was an important safety feature for the Convair 990 Landing System Research Aircraft, which tested space shuttle tires. It was imperative to know the extreme conditions the shuttle tires could tolerate at landing without putting the shuttle and its crew at risk. In addition, the CV990 was able to land repeatedly to test the tires. The TAV was built from a kit and modified into a radio controlled, video-equipped machine to drill holes in aircraft test tires that were in imminent danger of exploding because of one or more conditions: high air pressure, high temperatures, and cord wear. An exploding test tire releases energy equivalent to two and one-half sticks of dynamite and can cause severe injuries to anyone within 50 ft. of the explosion, as well as ear injury - possibly permanent hearing loss - to anyone within 100 ft. The degree of danger is also determined by the temperature pressure and cord wear of a test tire. The TAV was developed by David Carrott, a PRC employee under contract to NASA.

1995-01-01

256

In vitro antioxidant activity of banana (Musa spp. ABB cv. Pisang Awak).  

PubMed

The methanolic extract of Musa ABB cv Pisang Awak was investigated for the polyphenolic contents and antioxidant activity. The total phenol and flavonoid contents of the fruit extract were found to be 120 mg gallic acid equivalents (GAE) and 440 mg quercetin equivalents (QE)/100 g of sample dry weight, respectively. The antioxidant activity of the Pisang Awak methanol extract (PAME) (20-500 microg/ml) was determined using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing capacity, 2-2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) radical cation decolourization and hydroxyl radical scavenging capacity (OH*). The EC50 values of DPPH, ABTS and OH* activities of the PAME and butylated hydroxy toluene (BHT) were found to be 65 and 9 microg/ml, 29 and 6 microg/ml, 36 and 42 microg/ml respectively. The reducing capacity increased with increasing concentration (31.5-1000 mg/ml) of the fruit extract and the activity was comparable with the standard BHT. The high performance thin layer chromatography (HPTLC) analysis of the extract revealed the presence of polyphenols. The strong and positive correlations were obtained between total phenol/flavonoid contents (R2 = 0.693-1.0) and free radical scavenging ability was attributed to the polyphenols as the major antioxidants. PMID:22650010

Darsini, D Teepica Priya; Maheshu, V; Vishnupriya, M; Sasikumar, J M

2012-04-01

257

Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures.  

PubMed

The plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. A cell suspension culture was developed from calli of grape leaves of Vitis vinifera cv. Negramaro in order to study the bioproduction of resveratrol. The effects of a number of secondary plant metabolism elicitors, namely chitosan, methyl jasmonate, jasmonic acid, coronatine, and 12-oxo-phytodienoic acid, were tested on this cell suspension culture. The identification and quantification of stilbenes was achieved with high performance liquid chromatography, with both spectrophotometric and mass spectrometric detection. Of the tested elicitors, methyl jasmonate was the most effective in inducing the biosynthesis of approximately 4 mg g(-1) dry weight (about 60 mg L(-1)) of resveratrol. Conversely, 12-oxo-phytodienoic acid, jasmonic acid, and coronatine were able to trigger the synthesis of approximately 20 mg g(-1) dry weight (200-210 mg L(-1)) of viniferins. Taken together, our results show for the first time different modulatory effects of closely-related jasmonates on stilbene biosynthesis. PMID:25674504

Taurino, Marco; Ingrosso, Ilaria; D'amico, Leone; De Domenico, Stefania; Nicoletti, Isabella; Corradini, Danilo; Santino, Angelo; Giovinazzo, Giovanna

2015-01-01

258

Biomonitoring of air pollution with mercury in Croatia by using moss species and CV-AAS.  

PubMed

Moss samples from four dominant species (Hypnum cupressiforme, Pleurozium schreberi, Homalothecium sericeum and Brachythecium rutabulum) were collected during the summer and autumn of 2010 from 121 sampling sites evenly distributed over the territory of Croatia. Samples were totally digested by using microwave digestion system, whilst mercury was analysed by using cold vapour atomic absorption spectrometry (CV-AAS). Descriptive statistics were done from analyses of mercury in all moss samples. The content of mercury ranged from 0.010 to 0.145 mg kg(-1) with a median value of 0.043 mg kg(-1). Hg distribution map shows the sites of the country with higher levels of this element. High contents of Hg were found in moss samples collected from the regions of Podravina and Istria as a result of anthropogenic pollution. Comparison of median values and ranges with those found in moss samples in 2006 shows slight reduction of mercury air pollution. When compared to the results obtained from recent studies conducted in Slovenia, Macedonia and especially in Norway-which serves as a reference considering the fact that it is a pristine area-mercury air pollution in Croatia is insignificant. PMID:24733436

Spiri?, Zdravko; Vu?kovi?, Ivana; Stafilov, Traj?e; Kušan, Vladimir; Ba?eva, Katerina

2014-07-01

259

Characterization of Fusarium wilt resistant somaclonal variants of banana cv. Rasthali by cDNA-RAPD.  

PubMed

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is counted among the most destructive diseases of crop plants in India. In the absence of any credible control measure to manage this disease, development of resistant cultivars is the best option. Somaclonal variations arising out of long term in vitro culture of plant tissues is an important source of genetic variability and the selection of somaclones having desired characteristics is a promising strategy to develop plants with improved characters. In the present study, we isolated a group of somaclonal variants of banana cv. Rasthali which showed efficient resistance towards Foc race 1 infection in repeated bioassays. cDNA-RAPD methodology using 96 decamer primers was used to characterize these somaclonal variants. Among the four differentially amplified bands obtained, one mapping to the coding region of a lipoxygenase gene was confirmed to be down regulated in the somaclones as compared to controls by real-time quantitative RT-PCR. Our results correlated well with earlier studies with lipoxygenase mutants in maize wherein reduced expression of lipoxygenase led to enhanced resistance towards Fusarium infection. PMID:25160909

Ghag, Siddhesh B; Shekhawat, Upendra K S; Ganapathi, Thumballi R

2014-12-01

260

Influence of vine vigor on grape (Vitis vinifera L. Cv. Pinot Noir) and wine proanthocyanidins.  

PubMed

The relationships between variations in grapevine (Vitis vinifera L. cv. Pinot noir) growth and resulting fruit and wine phenolic composition were investigated. The study was conducted in a commercial vineyard consisting of the same clone, rootstock, age, and vineyard management practices. The experimental design involved monitoring soil, vine growth, yield components, and fruit composition (soluble solids, flavan-3-ol monomers, proanthocyanidins, and pigmented polymers) on a georeferenced grid pattern to assess patterns in growth and development. Vine vigor parameters (trunk cross-sectional area, average shoot length, and leaf chlorophyll) were used to delineate zones within both blocks to produce research wines to investigate the vine-fruit-wine continuum. There was no significant influence of vine vigor on the amount of proanthocyanidin per seed and only minimal differences in seed proanthocyanidin composition. However, significant increases were found in skin proanthocyanidin (mg/berry), proportion of (-)-epigallocatechin, average molecular mass of proanthocyanidins, and pigmented polymer content in fruit from zones with a reduction in vine vigor. In the wines produced from low-vigor zones, there was a large increase in the proportion of skin tannin extracted into the wine, whereas little change occurred in seed proanthocyanidin extraction. The level of pigmented polymers and proanthocyanidin molecular mass were higher in wines made from low-vigor fruit compared to wines made from high-vigor fruit, whereas the flavan-3-ol monomer concentration was lower. PMID:15998151

Cortell, Jessica M; Halbleib, Michael; Gallagher, Andrew V; Righetti, Timothy L; Kennedy, James A

2005-07-13

261

Stable MSAP markers for the distinction of Vitis vinifera cv Pinot noir clones.  

PubMed

Grapevine is one of the most economically important fruit crops. Molecular markers have been used to study grapevine diversity. For instance, simple sequence repeats are a powerful tool for identification of grapevine cultivars, while amplified fragment length polymorphisms have shown their usefulness in intra-varietal diversity studies. Other techniques such as sequence-specific amplified polymorphism are based on the presence of mobile elements in the genome, but their detection lies upon their activity. Relevant attention has been drawn toward epigenetic sources of variation. In this study, a set of Vitis vinifera cv Pinot noir clones were analyzed using the methylation-sensitive amplified polymorphism technique with isoschizomers MspI and HpaII. Nine out of fourteen selective primer combinations were informative and generated two types of polymorphic fragments which were categorized as "stable" and "unstable." In total, 23 stable fragments were detected and they discriminated 92.5 % of the studied clones. Detected stable polymorphisms were either common to several clones, restricted to a few clones or unique to a single clone. The identification of these stable epigenetic markers will be useful in clonal diversity studies. We highlight the relevance of stable epigenetic variation in V. vinifera clones and analyze at which level these markers could be applicable for the development of forthright techniques for clonal distinction. PMID:23723045

Ocaña, Juan; Walter, Bernard; Schellenbaum, Paul

2013-11-01

262

Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.  

PubMed

Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to approximately 36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants. PMID:16604461

Kanamoto, Hirosuke; Yamashita, Atsushi; Asao, Hiroshi; Okumura, Satoru; Takase, Hisabumi; Hattori, Masahira; Yokota, Akiho; Tomizawa, Ken-Ichi

2006-04-01

263

The Effect of the Arabic Syllabic Prosodic Features on the Production of English CV Sequences  

E-print Network

The aim of this research is to investigate the effect of the Arabic minimal prosodic unit (the syllable) on the production of English L2 CV sequences. To study the influence of phonetic context upon a given segment and the overall effect the Arabic syntagmatic features on the Arab learner's ability to reorganize in terms of English patterns, samples of conversational exchanges of some native speakers of adult/child Jordanian Arabic were recorded over a two month period. The syllable patterns that regulate the phonological organization of the conversational exchanges were described. To study the effect of the preceding and following consonants on the adjacent vowel sounds, two sets of consonantal contexts were examined: 'emphatic ' vs. 'non-emphatic'. The objectives of this study are the following: (i) to identify prosodic features which span the internal structure of the Arabic word templates, e.g. the vocalic melody of frontness vs. backness (ii) to explore the Arabic language-specific phonological constraints and to determine their effect on the production of English sound patterns (iii) to study the Arabic templates which are similar in shape to English equivalents, because contrastive linguistics studies, to date, have so far focused on phonological negative interlingual transfer to the effective exclusion of positive interlingual transfer. (vi) to demonstrate how English utterances are restructured so that the constituent syllables follow typical Arabic syllabication.

Dr. Mohammad Anani

264

A New Modification of C-V Flap Technique in Nipple Reconstruction: Rolled Triangular Dermal-Fat Flaps.  

PubMed

In this study, a simple modification of the C-V flap technique designed to maintain the neo-nipple projection performed as the last stage of breast reconstruction is described. The technique was used in seventeen patients who were treated with breast reconstruction using the transverse rectus abdominis flap. Except for one patient with a 2.3 mm decrease in nipple projection, the projection of all neo-nipples was preserved at the end of the mean follow-up period of 19.4 months. According to the results of our study, modifying the C-V flap technique by adding rolled triangular dermal-fat flaps improves the long-term maintenance of neo-nipple projection. PMID:25428272

Temiz, Gökhan; Ye?ilo?lu, Nebil; Sirino?lu, Hakan; Sarici, Murat

2014-11-27

265

Improved Antioxidant and Anti-inflammatory Potential in Mice Consuming Sour Cherry Juice ( Prunus Cerasus cv. Maraska)  

Microsoft Academic Search

The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and\\u000a anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and\\u000a brain), LPO (liver, brain)

Ana Šari?; Sandra Sobo?anec; Tihomir Balog; Borka Kuši?; Višnja Šverko; Verica Dragovi?-Uzelac; Branka Levaj; Zrinka ?osi?; Željka Ma?ak Šafranko; Tatjana Marotti

2009-01-01

266

The influence of storage on aroma, soluble solids, acid and colour of sour cherries (Prunus cerasus L.) cv. Stevnsbær  

Microsoft Academic Search

The present study involved a laboratory scale experiment where the impact of post-harvest storage on the quality of sour\\u000a cherries (Prunus cerasus L.) cv Stevnsb?r was investigated. Cherries were stored for 7 days at temperatures of 2???C, 10???C, 20???C, and 30???C,\\u000a and at 20???C in combination with a 20% CO2 atmosphere. Cherry quality was assessed by analysis of soluble solids,

M. B. Petersen; L. Poll

1999-01-01

267

Identification of culturable and originally non-culturable endophytic bacteria isolated from shoot tip cultures of banana cv. Grand Naine  

Microsoft Academic Search

In this article we describe the identification of endophytic bacteria belonging to three groups isolated from shoot tip cultures\\u000a of banana cv. Grand Naine in a recent study (Thomas et al. 2008) based on partial 16S rRNA gene sequence homology analysis. The first group included banana stocks that displayed obvious\\u000a colony growth on MS based tissue culture medium during the first

Pious Thomas; Ganiga K. Swarna; Pulak K. Roy; Prakash Patil

2008-01-01

268

Preliminary research on conversion of encapsulated somatic embryos of Citrus reticulata Blanco, cv. Mandarino Tardivo di Ciaculli  

Microsoft Academic Search

Somatic embryogenesis was obtained through anther culture of Citrus reticulata, cv. Mandarino Tardivo di Ciaculli. The work was carried out to evaluate the response of somatic embryos inside a sodium\\u000a alginate coating to different storage periods, and to the effects of the germicide PPM (1 ml l?1) and the fungicide Thiophanate-methyl (100 mg l?1). The effect of these alone or in combination, added to

Germanà Maria Antonietta; Hafiz Ishfaq Ahmad; Micheli Maurizio; Standardi Alvaro

2007-01-01

269

Regeneration of plants from alginate-encapsulated somatic embryos of banana cv. Rasthali ( Musa SPP. AAB Group)  

Microsoft Academic Search

Summary  Somatic embryos of banana cv. Rasthali (AAB genomic group) were encapsulated in 5% sodium alginate to produce synthetic seeds.\\u000a The frequency of germination of ecapsulated embryos varied considerably on different gel matrices and substrates used for\\u000a plant development. Maximum conversion frequency of 66% was noted from encapsulated embryos cultured on MS medium. Plantlets\\u000a developed from synthetic seeds were successfully trnasferred

T. R. Ganapathi; L. Srinivas; P. Suprasanna; V. A. Bapat

2001-01-01

270

Scheduling drip irrigation for fall tomatoes (Lycopersicon esculentum Mill. cv. Sunny) in the Lower Rio Grande Valley  

E-print Network

of Advisory Committee: Dr. Grant Vest Two methods of scheduling drip irrigation for tomatoes (Lycopersican esculennJm Mill cv. Sunny) were studied on two soils in Weslaco, Texas during the fall of 1984. Irrigation at 20, 40 or 60% available soil water... Scheduling Evapotranspiration Evaporimeters to Schedule Irrigation Tensiometers in Irrigation Scheduling Precipitation Drip Irrigation Effects of Moisture Regimes on Tomatoes 2 3 5 7 8 9 11 III MATERIALS AND METHODS The Site Treatments...

Vargas Prieto, Alberto Miguel

1985-01-01

271

Respiration and nitrogen fixation of hydroponically cultured Phaseolus vulgaris L. cv. OAC Rico and a supernodulating mutant  

Microsoft Academic Search

This study was undertaken to determine how carbon utilization of fruit production might affect symbiotic activity in hydroponically cultured white bean (Phaseolus vulgaris L.) cv. Rico and its supernodulating genotype R32-BS15 (abbreviated as RBS15). Total plant biomass production of both genotypes was similar. Nodule dry weight of RBS15 consistently scored approximately twice the amount recorded for Rico, while nodule numbers

Alexander P. Hansen; Tadakatsu Yoneyama; Hiroshi Kouchi; Peter Martin

1993-01-01

272

Gibberellins in developing fruits of Pisum sativum cv. Alaska: Studies on their role in pod growth and seed development  

Microsoft Academic Search

Gibberellins A1, A8, A20 and A29 were identified by capillary gas chromatography-mass spectrometry in the pods and seeds from 5-d-old pollinated ovaries of pea (Pisum sativum cv. Alaska). These gibberellins were also identified in 4-d-old non-developing, parthenocarpic and pollinated ovaries. The level of gibberellin A1 within these ovary types was correlated with pod size. Gibberellin A1, applied to emasculated ovaries

J. L. Garcia-Martinez; V. M. Sponsel; P. Gaskin

1987-01-01

273

Agrobacterium -mediated transformation of cotton ( Gossypium hirsutum L. cv. Zhongmian 35 ) using glyphosate as a selectable marker  

Microsoft Academic Search

The most economically significant Chinese cotton cultivar (Gossypium hirsutum L. cv. Zhongmian 35) was transformed via Agrobacterium tumefaciens-mediated DNA transfer. The aroA-M1 gene that confers resistance to the glyphosate was fused with a chloroplast-transit peptide of Arabidopsis thaliana 5-enolpyruvyl-3-phosphoshikimate synthase (ASP) and expressed in cotton plants under the control of a CaMV35S promoter. Transgenic plants were directly selected on medium

Fu-Yong Zhao; Yun-Feng Li; Peilin Xu

2006-01-01

274

Effects of alkalis, oxidants and urea on the nutritive value of rhodes grass ( Chloris gayana cv. Callide)  

Microsoft Academic Search

Two in sacco experiments were conducted to evaluate the impact on the nutritive value of rhodes grass hay (Chloris gayana cv. Callide) of treatment with alkalis or oxidants. In Experiment 1, three alkalis (Ca(OH)2, NaOH, CaO) and two oxidants (NaOCl and H2O2) were applied at levels of 0, 20, 40, 60 or 80g\\/kg of dry matter (DM). NaOH, Ca(OH)2 and

B. C Granzin; G. McL Dryden

2003-01-01

275

Investigation of the cholesterol-lowering action of insoluble fibre derived from the peel of Citrus sinensis L. cv. Liucheng  

Microsoft Academic Search

A water-insoluble fibre-rich fraction (WIFF) was isolated from the peel of Citrus sinensis L. cv. Liucheng. The effects of a WIFF-containing diet on lipid and cholesterol absorption in hamsters were investigated and compared with those of a cellulose-containing diet and fibre-free diet, as controls. Results demonstrated that WIFF could significantly (P<0.05) decrease the levels of serum triglyceride, serum total cholesterol,

C.-F. Chau; Y.-L. Huang; C.-Y. Lin

2004-01-01

276

An efficient protoplast-to-plant system for the hybrid ornamental shrub, Weigela × florida cv. Bristol Ruby ( Caprifoliaceae )  

Microsoft Academic Search

Strategies were developed for the successful isolation of large numbers of highly viable protoplasts from the leaves, stems\\u000a and roots of axenic plants of the hybrid ornamental shrubWeigela florida cv Bristol Ruby. Protoplasts, of all sources, were cultured on different media, leading to the establishment of sustained\\u000a divisions, and coupled with the production of multi-celled (>50 cells) colonies. However, those

Sergio J. Ochatt

1993-01-01

277

OpenCV C# wrapper based video enhancement using different optical flow methods in the super-resolution  

Microsoft Academic Search

This article presents a simple method on how to implement a super-resolution based video enhancement technique in .NET using the functions of the OpenCV library. First, we outline the goal of this project and after that, a short review of the steps of super-resolution technique is given. As a part of the discussion about the concrete implementation, firstly the general

A. Nagy; Z. Vamossy

2008-01-01

278

The Indirect Keyboard Control System by Using the Gaze Tracing Based on Haar Classifier in OpenCV  

Microsoft Academic Search

This paper describes on the indirect interface system in which general users assign computer instructions just through the gaze tracing, without mouse or keyboard. We use the Web camera to replace the computer-input system. The face region and the eye region was extracted based on Haar classifier implemented on open source computer vision library (OpenCV). It controls mouse-moving by automatically

Chang-Zheng Li; Chung-Kyue Kim; Jong-Seung Park

2009-01-01

279

Anaerobic n-Alkane Metabolism by a Sulfate-Reducing Bacterium, Desulfatibacillum aliphaticivorans Strain CV2803T  

Microsoft Academic Search

The alkane-degrading, sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T, recently isolated from marine sediments, was investigated for n-alkane metabolism. The total cellular fatty acids of this strain had predominantly odd numbers of carbon atoms (C odd) when the strain was grown on a C-odd alkane (pentadecane) and even numbers of carbon atoms (C even) when it was grown on a C-even

Cristiana Cravo-Laureau; Vincent Grossi; Danielle Raphel; Robert Matheron; Agnes Hirschler-Rea

2005-01-01

280

Changes in volatile compounds of Habanero chile pepper ( Capsicum chinense Jack. cv. Habanero) at two ripening stages  

Microsoft Academic Search

The steam volatile components of Yucatan Habanero chile pepper (Capsicum chinense Jack. cv. Habanero) at two ripening stages (green and orange) were analyzed using GC and GC\\/MS. Both samples had several compounds in common. One hundred and two compounds were identified, from which (E)-2-hexenal, hexyl 3-methylbutanoate, (Z)-3-hexenyl 3-methylbutanoate, hexyl pentanoate, 3,3-dimethylcyclohexanol, and hexadecanoic acid were found to be the major

Jorge Pino; Enrique Sauri-Duch; Rolando Marbot

2006-01-01

281

Improved antioxidant and anti-inflammatory potential in mice consuming sour cherry juice (Prunus Cerasus cv. Maraska).  

PubMed

The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties. PMID:19763832

Sari?, Ana; Sobocanec, Sandra; Balog, Tihomir; Kusi?, Borka; Sverko, Visnja; Dragovi?-Uzelac, Verica; Levaj, Branka; Cosi?, Zrinka; Macak Safranko, Zeljka; Marotti, Tatjana

2009-12-01

282

Self Consistent Simulation of C-V Characterization and Ballistic Performance of Double Gate SOI Flexible-FET Incorporating QM Effects  

E-print Network

Capacitance-Voltage (C-V) & Ballistic Current- Voltage (I-V) characteristics of Double Gate (DG) Silicon-on- Insulator (SOI) Flexible FETs having sub 35nm dimensions are obtained by self-consistent method using coupled Schrodinger- Poisson solver taking into account the quantum mechanical effects. Although, ATLAS simulations to determine current and other short channel effects in this device have been demonstrated in recent literature, C-V & Ballistic I-V characterizations by using self-consistent method are yet to be reported. C-V characteristic of this device is investigated here with the variation of bottom gate voltage. The depletion to accumulation transition point (i.e. Threshold voltage) of the C-V curve should shift in the positive direction when the bottom gate is negatively biased and our simulation results validate this phenomenon. Ballistic performance of this device has also been studied with the variation of top gate voltage.

Zubair Al Azim; Nadim Chowdhury; Iftikhar Ahmad Niaz; Md. Hasibul Alam; Imtiaz Ahmed; Quazi D. M. Khosru

2012-11-16

283

Asteroid Lightcurve Analysis at Riverland Dingo Observatory (RDO): 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV 26.  

NASA Astrophysics Data System (ADS)

Lightcurves for five asteroids selected from the Collaborative Asteroid Lightcurve Link (CALL) were obtained at Riverland Dingo Observatory (RDO) from 2012 July-September: 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV26.

Hills, Kevin

2013-01-01

284

Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston 1  

PubMed Central

Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived (MD) embryo culture system. TAGs accumulating during embryo development exhibited changes in acyl composition similar to those observed in developing zygotic embryos of the same cv, particularly with respect to erucic and eicosenoic acids. However, MD embryos showed a much higher rate of incorporation of 14C-erucoyl moieties into TAGs in vitro than zygotic embryos. Homogenates of early-late cotyledonary stage MD embryos (14-29 days in culture) were assessed for the ability to incorporate 22:1 and 18:1 (oleoyl) moieties into glycerolipids. In the presence of [1-14C]22:1-coenzyme A (CoA) and various acyl acceptors, including glycerol-3-phosphate (G-3-P), radiolabeled erucoyl moieties were rapidly incorporated into the TAG fraction, but virtually excluded from other Kennedy Pathway intermediates as well as complex polar lipids. This pattern of erucoyl incorporation was unchanged during time course experiments or upon incubation of homogenates with chemicals known to inhibit Kennedy Pathway enzymes. In marked contrast, parallel experiments conducted using [1-14C]18:1-CoA and G-3-P indicated that 14C oleoyl moieties were incorporated into lyso-phosphatidic acids, phosphatidic acids, diacylglycerols, and TAGs of the Kennedy Pathway, as well as other complex polar lipids, such as phosphatidylcholines and phosphatidylethanolamines. When supplied with l-[2-3H(N)]G-3-P and [1-14C]22:1-CoA, the radiolabeled TAG pool contained both isotopes, indicating G-3-P to be a true acceptor of erucoyl moieties. Radio-high-performance liquid chromatography, argentation thin-layer chromatography/gas chromatography-mass spectrometry, and stereospecific analyses of radiolabeled TAGs indicated that 22:1 was selectively incorporated into the sn-3 position by a highly active diacylglycerol acyltransferase (DGAT; EC 2.3.1.20), while oleoyl moieties were inserted into the sn-1 and sn-2 positions. In the presence of sn-1,2-dierucin and [1-14C]22:1-CoA, homogenates and microsomal preparations were able to produce radiolabeled trierucin, a TAG not found endogenously in this species. A 105,000g pellet fraction contained 22:1-CoA:DGAT exhibiting the highest specific activity. The rate of 22:1-CoA:DGAT activity in vitro could more than account for the maximal rate of TAG biosynthesis observed in vivo during embryo development. In double label experiments, G-3-P was shown to stimulate the conversion of [3H]phosphatidylcholines to [3H]diacylglycerols, which subsequently acted as acceptors for 14C erucoyl moieties. In vitro, 22:1 moieties did not enter the sn-1 position of TAGs by a postsynthetic modification or transacylation of preformed TAGs. PMID:16668417

Taylor, David C.; Weber, Nikolaus; Barton, Dennis L.; Underhill, Edward W.; Hogge, Lawrence R.; Weselake, Randall J.; Pomeroy, M. Keith

1991-01-01

285

Indicators of aqueous alteration and thermal metamorphism on the CV parent body: Microtextures of a dark inclusion from Allende  

NASA Astrophysics Data System (ADS)

An unusual dark clast in the Allende CV3 chondrite (termed Allende-AF), which was previously interpreted as a primary aggregate formed in the solar nebula (Kurat et al., 1989; Palme et al., 1989), was re-examined. Our study reveals abundant evidence suggesting that it probably experienced extensive aqueous alteration and subsequent thermal metamorphism on the meteorite parent body. Allende-AF contains numerous rounded to oval-shaped inclusions embedded in a dark matrix. The inclusions, consisting predominantly of fine grains of Fe-rich olivine, have internal textures suggesting that they are pseudomorphs after chondrules. Several inclusions appear to be replaced CAIs. Veins filled with fibrous olivine grains occur abundantly in both inclusions and matrix; some veins (up to 4 mm in length) penetrate several inclusions, providing strong evidence that aqueous alteration occurred after accretion. The fibrous morphology of olivine in veins and inclusions suggests that the olivine was produced by dehydration and thermal transformation of phyllosilicate that had been formed by aqueous alteration. Olivine grains in the matrix contain numerous micro-inclusions of Fe?Ni sulfide, which were probably incorporated during transformation from phyllosilicate. Allende-AF is probably related to the fine-grained variety of dark inclusions reported from CV3 chondrites that has been described as the type containing abundant porous aggregates of Fe-rich olivine by Johnson et al. (1990). Many dark inclusions previously described appear to be similar in texture and mineralogy to Allende-AF, and probably experienced similar secondary process on the meteorite parent body. The wide variation in texture of dark inclusions can be explained by different degrees of aqueous alteration that preceded thermal metamorphism. The size distribution of chondrule pseudomorphs and the abundance of CAI pseudomorphs suggest that the precursor of Allende-AF is a CV type chondrite, probably Allende itself. Oxygen isotopic and chemical compositions are consistent with this interpretation. The CV parent body has been commonly thought to have escaped major secondary processing. However, Allende-AF provides evidence that extensive aqueous alteration and thermal metamorphism have occurred locally on the CV parent body.

Kojima, Tomoko; Tomeoka, Kazushige

1996-07-01

286

How will climate change influence grapevine cv. Tempranillo photosynthesis under different soil textures?  

PubMed

While photosynthetic responses to elevated CO2, elevated temperature, or water availability have previously been reported for grapevine as responses to single stress factors, reports on the combined effect of multiple stress factors are scarce. In the present work, we evaluated effects of simulated climate change [CC; 700 ppm CO2, 28/18 °C, and 33/53 % relative humidity (RH), day/night] versus current conditions (375 ppm CO2, 24/14 °C, and 45/65 % RH), water availability (well-irrigated vs. water deficit), and different types of soil textures (41, 19, and 8 % of soil clay contents) on grapevine (Vitis vinifera L. cv. Tempranillo) photosynthesis. Plants were grown using the fruit-bearing cutting model. CC increased the photosynthetic activity of grapevine plants grown under well-watered conditions, but such beneficial effects of elevated CO2, elevated temperature, and low RH were abolished by water deficit. Under water-deficit conditions, plants subjected to CC conditions had similar photosynthetic rates as those grown under current conditions, despite their higher sub-stomatal CO2 concentrations. As expected, water deficit reduced photosynthetic activity in association with inducing stomatal closure that prevents water loss. Evidence for photosynthetic downregulation under elevated CO2 was observed, with decreases in photosynthetic capacity and leaf N content and increases in the C/N ratio in plants subjected to CC conditions. Soil texture had no marked effects on photosynthesis and did not modify the photosynthetic response to CC and water-deficit conditions. However, in mature well-irrigated plants grown in the soils with the highest sand content, an important decrease in stomatal conductance was observed as well as a slight decrease in the utilization of absorbed light in photosynthetic electron transport (measured as photochemical quenching), possibly related to a low water-retention capacity of these soils even under well-watered conditions. PMID:25786733

Leibar, Urtzi; Aizpurua, Ana; Unamunzaga, Olatz; Pascual, Inmaculada; Morales, Fermín

2015-05-01

287

Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane 1  

PubMed Central

Glucocerebrosides of whole rye (Secale cerale L. cv Puma) leaf and plasma membrane were analyzed using gas chromatography-mass spectrometry and gas chromatography following hydrolysis or as intact molecules purified by reverse-phase high performance liquid chromatography. Fatty acids of acid-hydrolyzed leaf and plasma membrane glucocerebrosides consisted of >98 weight percent saturated and monounsaturated 2-hydroxy fatty acids which contained 16 to 26 carbon atoms. The major fatty acids detected were 2-hydroxynervonic acid (24:1h), 2-hydroxylignoceric acid (24:0h), 2-hydroxyerucic acid (22:1h), and 2-hydroxybehenic acid (22:0h). Long-chain bases of alkaline-hydrolyzed glucocerebrosides consisted primarily of cis-trans isomers of the trihydroxy base 4-hydroxysphingenine (t18:1) and the dihydroxy base sphingadienine (d18:2) with lesser amounts of 4-hydroxysphinganine (t18:0) and isomers of sphingenine (d18:1). Intact, underivatized glucocerebroside molecular species of rye leaf and plasma membrane were separated into more than 30 molecular species using reverse-phase HPLC. The molecular species composition of leaf and plasma membrane were quantitatively and qualitatively similar. The major molecular species was 24:1h-t18:1 which constituted nearly 40 weight percent of leaf and plasma membrane extracts. Several other species including 22:1h-t18:1, 24:1h-t18:1 (isomer), 22:0h-t18:1, 24:1h-d18:2, and 24:0h-t18:1 each comprised 4 to 8% of the total. It is anticipated that the high performance liquid chromatography procedure developed in this study to separate intact, underivatized lipid molecular species will be useful in future studies of the physical properties and biosynthesis of plant glucocerebrosides. PMID:16667981

Cahoon, Edgar B.; Lynch, Daniel V.

1991-01-01

288

Erwinia amylovora affects the phenylpropanoid-flavonoid pathway in mature leaves of Pyrus communis cv. Conférence.  

PubMed

Flavonoids, which are synthesized by the phenylpropanoid-flavonoid pathway, not only contribute to fruit colour and photoprotection, they also may provide antimicrobial and structural components during interaction with micro-organisms. A possible response of this pathway was assessed in both mature and immature leaves of shoots of 2-year-old pear trees cv. Conférence, which were inoculated with the gram-negative bacterium Erwinia amylovora strain SGB 225/12, were mock-inoculated or were left untreated. The phenylpropanoid-flavonoid pathway was analysed by histological studies, by gene expression using RT-qPCR and by HPLC analyses of the metabolites at different time intervals after infection. Transcription patterns of two key genes anthocyanidin reductase (ANR) and chalcone synthase (CHS) related to the phenylpropanoid-flavonoid pathway showed differences between control, mock-inoculated and E. amylovora-inoculated mature leaves, with the strongest reaction 48 h after inoculation. The impact of E. amylovora was also visualised in histological sections, and confirmed by HPLC, as epicatechin -which is produced via ANR- augmented 72 h after inoculation in infected leaf tissue. Besides the effect of treatments, ontogenesis-related differences were found as well. The increase of certain key genes, the rise in epicatechin and the visualisation in several histological sections in this study suggest a non-negligible impact on the phenylpropanoid-flavonoid pathway in Pyrus communis due to inoculation with E. amylovora. In this study, we propose a potential role of this pathway in defence mechanisms, providing a detailed analysis of the response of this system attributable to inoculation with E. amylovora. PMID:23582642

Vrancken, K; Holtappels, M; Schoofs, H; Deckers, T; Treutter, D; Valcke, R

2013-11-01

289

[Effect of CV-2619 (idebenone) on the half-life and hemolysis of red blood cells in stroke-prone spontaneously hypertensive rats (SHRSP)].  

PubMed

The effects of CV-2619 on the half-life and hemolysis of red blood cells (RBC) in stroke-prone spontaneously hypertensive rats (SHRSP) were examined. The half-life of RBC in SHRSP was shorter than that in control Wistar Kyoto rats (WKY) and was significantly prolonged in SHRSP kept on a diet containing 0.1% (w/w) of CV-2619 (calculated at 71.0 mg/kg/day): 11.7 +/- 0.4 days in untreated SHRSP (n = 11); 13.8 +/- 0.1 days in treated SHRSP (n = 5, P less than 0.01); and 14.8 +/- 0.5 days in WKY (n = 6). The hemolysis of RBC in salt-loaded SHRSP was accelerated compared with that in WKY. In SHRSP given CV-2619 (20 or 70 mg/kg/day, p.o.) for 2 weeks, the hemolysis was significantly inhibited; the percent hemolysis was 43.9 +/- 0.9% (n = 10) in the control, 39.5 +/- 0.9% (n = 9, P less than 0.01) in the group given 20 mg/kg CV-2619, and 37.1 +/- 0.8% (n = 9, P less than 0.001) in the group given 70 mg/kg CV-2619. These results suggest that the stabilizing effect of CV-2619 on the membrane of RBC is involved in its therapeutic effects in cerebral vascular disorders. PMID:6510844

Suno, M; Shibota, M; Nagaoka, A

1984-10-01

290

Mercury Abundances and Isotopic Compositions in the Murchison (CM) and Allende (CV)Carbonaceous Chondrites  

NASA Technical Reports Server (NTRS)

The abundance and isotopic composition of Hg was determined in bulk samples of both the Murchison (CM) and Allende (CV) carbonaceous chondrites using single- and multi-collector inductively coupled plasma mass spectrometry (ICP-MS). The bulk abundances of Hg are 294 6 15 ng/g in Murchison and 30.0 6 1.5 ng/g in Allende. These values are within the range of previous measurements of bulk Hg abundances by neutron activation analysis (NAA). Prior studies suggested that both meteorites contain isotopically anomalous Hg, with d l 96/202Hg values for the anomalous, thermal-release components from bulk samples ranging from 2260 %o to 1440 9/00 in Murchison and from 2620 9/00 to 1540 9/00 in Allende (Jovanovic and Reed, 1976a; 1976b; Kumar and Goel, 1992). Our multi-collector ICP-MS measurements suggest that the relative abundances of all seven stable Hg isotopes in both meteorites are identical to terrestrial values within 0.2 to 0.5 9/00m. On-line thermal-release experiments were performed by coupling a programmable oven with the singlecollector ICP-MS. Powdered aliquots of each meteorite were linearly heated from room temperature to 900 C over twenty-five minutes under an Ar atmosphere to measure the isotopic composition of Hg released fiom the meteorites as a h c t i o n of temperature. In separate experiments, the release profiles of S and Se were determined simultaneously with Hg to constrain the Hg distribution within the meteorites and to evaluate the possibility of Se interferences in previous NAA studies. The Hg-release patterns differ between Allende and Murchison. The Hg-release profile for Allende contains two distinct peaks, at 225" and 343"C, whereas the profile for Murchison has only one peak, at 344 C. No isotopically anomalous Hg was detected in the thermal-release experiments at a precision level of 5 to 30 9/00, depending on the isotope ratio. In both meteorites the Hg peak at ;340"C correlates with a peak in the S-release profile. This correlation suggests that Hg is associated with S-bearing phases and, thus, that HgS is a major Hg-bearing phase in both meteorites. The Hg peak at 225 C for Allende is similar to release patterns of physically adsorbed Hg on silicate and metal grains.

Lauretta, D. S.; Klaue, B.; Blum, J. D.; Buseck, P. R.

2001-01-01

291

Identification, quantitation and distribution of gibberellins in fruits of Pisum sativum L. cv. Alaska during pod development  

Microsoft Academic Search

In addition to the previously-reported gibberellins: GA1; GA8, GA20 and GA29 (García-Martínez et al., 1987, Planta 170, 130–137), GA3 and GA19 were identified by combined gas chromatography-mass spectrometry in pods and ovules of 4-d-old pollinated pea (Pisum sativum cv. Alaska) ovaries. Pods contained additionally GA17, GA81 (2a-hydroxy GA20) and GA29-catabolite. The concentrations of GA1, GA3, GA8, GA19, GA20 and GA29

José L. García-Martinez; Cristina Santes; Stephen J. Croker; Peter Hedden

1991-01-01

292

Opaque Mineral Assemblages at Chondrule Boundaries in the Vigarano CV Chondrite: Evidence for Gas-Solid Reactions Following Chondrule Formation  

NASA Technical Reports Server (NTRS)

Recent studies of opaque minerals in primitive ordinary chondrites suggest that metal grains exposed at chondrule boundaries were corroded when volatile elements recondensed after the transient heating event responsible for chondrule formation. Metal grains at chondrule boundaries in the Bishunpur (LL3.1) chondrite are rimmed by troilite and fayalite. If these layers formed by gas solid reaction, then the composition of the corrosion products can provide information on the chondrule formation environment. Given the broad similarities among chondrules from different chondrite groups, similar scale layers should occur on chondrules in other primitive meteorite groups. Here I report on metal grains at chondrule boundaries in Vigarano (CV3).

Lauretta, Dante S.

2004-01-01

293

[Transformation of embryogenic Calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) mediated by agrobacterium].  

PubMed

Formation of embryogenic calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) was induced from mature seeds as explants, and proliferated on MS medium containing 2,4-D 5.0 mg/L and KT 0.05 mg/L. An effective and stable callus regeneration system was established by optimizing the culture conditions (Tables 1, 2 and Fig.2). After the calli were subcultured 8 weeks, selected the whitish-yellow-coloured compact nodular calli that transformed with plasmid pCAMBIA1304 carrying hygromycin resistance gene (hptII) and Pseudomonas pseudoalcaligenes insecticidal protein gene (ppIP), which was mediated by an Agrobacterium strain EHA105. Resistant plants were obtained after hygromycin selection (Figs.3, 4). Some important factors that affect the transformation efficiency were studied, which included selection pressure, time of embryogenic calli proliferation, OD value of Agrobacterium suspension, temperature, medium and time of co-cultivation, and concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformation plant regeneration. This research is the first successful genetic transformation of Elymus sibiricus L. cv. Chuancao No.2 mediated by Agrobacterium. PMID:16477130

Li, Da-Xu; Zhang, Jie; Zhao, Jian; Zhang, Yi; Li, Li; Liu, Su-Jun; Chen, Fei; Yang, Zhi-Rong

2006-02-01

294

Interpretation of F106B and CV580 in-flight lightning data and form factor determination  

NASA Technical Reports Server (NTRS)

Two topics of in-flight aircraft/lightning interaction are addressed. The first is the analysis of measured data from the NASA F106B Thunderstorm Research Aircraft and the CV580 research program run by the FAA and Wright-Patterson Air Force Base. The CV580 data was investigated in a mostly qualitative sense, while the F106B data was subjected to both statistical and quantitative analysis using linear triggered lightning finite difference models. The second main topic is the analysis of field mill data and the calibration of the field mill systems. The calibration of the F106B field mill system was investigated using an improved finite difference model of the aircraft having a spatial resolution of one-quarter meter. The calibration was applied to measured field mill data acquired during the 1985 thunderstorm season. The experimental determination of form factors useful for field mill calibration was also investigated both experimentally and analytically. The experimental effort involved the use of conducting scale models and an electrolytic tank. An analytic technique was developed to aid in the understanding of the experimental results.

Rudolph, T.; Horembala, J.; Eriksen, F. J.; Weigel, H. S.; Elliott, J. R.; Parker, S. L.; Perala, R. A.

1989-01-01

295

Aqueous alteration of the Bali CV3 chondrite: evidence from mineralogy, mineral chemistry, and oxygen isotopic compositions.  

PubMed

A petrographic, geochemical, and oxygen isotopic study of the Bali CV3 carbonaceous chondrite revealed that the meteorite has undergone extensive deformation and aqueous alteration on its parent body. Deformation textures are common and include flattened chondrules, a well-developed foliation, and the presence of distinctive (100) planar defects in olivine. The occurrence of alteration products associated with the planar defects indicates that the deformation features formed prior to the episode of aqueous alteration. The secondary minerals produced during the alteration event include well-crystallized Mg-rich saponite, framboidal magnetite, and Ca-phosphates. The alteration products are not homogeneously distributed throughout the meteorite, but occur in regions adjacent to relatively unaltered material, such as veins of altered material following the foliation. The alteration assemblage formed under oxidizing conditions at relatively low temperatures (<100 degrees C). Altered regions in Bali have higher Na, Ca, and P contents than unaltered regions which suggests that the fluid phase carried significant dissolved solids. Oxygen isotopic compositions for unaltered regions in Bali fall within the field for other CV3 whole-rocks, however, the oxygen isotopic compositions of the heavily altered material lie in the region for the CM and CR chondrites. The heavy-isotope enrichment of the altered regions in Bali suggest alteration conditions similar to those for the petrographic type-2 carbonaceous chondrites. PMID:11539152

Keller, L P; Thomas, K L; Clayton, R N; Mayeda, T K; DeHart, J M; McKay, D S

1994-12-01

296

Potential for phytoextraction of copper by Sinapis alba and Festuca rubra cv. Merlin grown hydroponically and in vineyard soils.  

PubMed

The extensive use of copper-bearing fungicides in vineyards is responsible for the accumulation of copper (Cu) in soils. Grass species able to accumulate Cu could be cultivated in the vineyard inter-rows for copper phytoextraction. In this study, the capacity of Festuca rubra cv Merlin and Sinapis alba to tolerate and accumulate copper (Cu) was first investigated in a hydroponic system without the interference of soil chemical-physical properties. After the amendment of Cu (5 or 10 mg Cu l-(1)) to nutrient solution, shoot Cu concentration in F. rubra increased up to 108.63 mg Cu kg(-1) DW, more than three times higher than in S. alba (31.56 mg Cu kg(-1) DW). The relationship between Cu concentration in plants and external Cu was dose-dependent and species specific. Results obtained from the hydroponic experiment were confirmed by growing plants in pots containing soil collected from six Italian vineyards. The content of soil organic matter was crucial to enhance Cu tolerance and accumulation in the shoot tissues of both plant species. Although S. alba produced more biomass than F. rubra in most soils, F. rubra accumulated significantly more Cu (up to threefold to fourfold) in the shoots. Given these results, we recommended that F. rubra cv Merlin could be cultivated in the vineyard rows to reduce excess Cu in vineyard soils. PMID:24234763

Malagoli, Mario; Rossignolo, Virginia; Salvalaggio, Nico; Schiavon, Michela

2014-03-01

297

Description of Gas-Phase Ion/Neutral Interactions in Differential Ion Mobility Spectrometry: CV Prediction Using Calibration Runs  

NASA Astrophysics Data System (ADS)

Differential ion mobility spectrometry (DMS) coupled to mass spectrometry is increasingly used in both quantitative analyses of biological samples and as a means of removing background interferences for enhanced selectivity and improved quality of mass spectra. However, DMS separation efficiency using dry inert gases often lacks the required selectivity to achieve baseline separation. Polar gas-phase modifiers such as alcohols are therefore frequently employed to improve selectivity via clustering/declustering processes. The choice of an optimal modifier currently relies on trial and error experiments, making method development a tedious activity. It was the goal of this study to establish a means of CV prediction for compounds using a homologous series of alcohols as gas-phase modifiers. This prediction was based on linear regression of compensation voltages of two calibration runs for the alcohols with the lowest and the highest molecular weights and readily available descriptors such as proton affinity and gas phase acidity of the modifier molecules. All experiments were performed on a commercial quadrupole linear ion trap mass spectrometer equipped with a DMS device between electrospray ionization source and entrance quadrupole lens. We evaluated our approach using a homologous series of 4-alkylbenzoic acids and a selection of 23 small molecules of high chemical diversity. Predicted CV values typically deviated from the experimentally determined values by less than 0.5 V. Several test compounds changed their ion mobility behavior for the investigated gas phase modifiers (e.g., from type B to type A) and thus could thus not be evaluated.

Auerbach, David; Aspenleiter, Julia; Volmer, Dietrich A.

2014-09-01

298

Processor core for real time background identification of HD video based on OpenCV Gaussian mixture model algorithm  

NASA Astrophysics Data System (ADS)

The identification of moving objects is a fundamental step in computer vision processing chains. The development of low cost and lightweight smart cameras steadily increases the request of efficient and high performance circuits able to process high definition video in real time. The paper proposes two processor cores aimed to perform the real time background identification on High Definition (HD, 1920 1080 pixel) video streams. The implemented algorithm is the OpenCV version of the Gaussian Mixture Model (GMM), an high performance probabilistic algorithm for the segmentation of the background that is however computationally intensive and impossible to implement on general purpose CPU with the constraint of real time processing. In the proposed paper, the equations of the OpenCV GMM algorithm are optimized in such a way that a lightweight and low power implementation of the algorithm is obtained. The reported performances are also the result of the use of state of the art truncated binary multipliers and ROM compression techniques for the implementation of the non-linear functions. The first circuit has commercial FPGA devices as a target and provides speed and logic resource occupation that overcome previously proposed implementations. The second circuit is oriented to an ASIC (UMC-90nm) standard cell implementation. Both implementations are able to process more than 60 frames per second in 1080p format, a frame rate compatible with HD television.

Genovese, Mariangela; Napoli, Ettore

2013-05-01

299

In vitro selection and field responses of somaclonal variant plants of rice cv PR113 for drought tolerance  

PubMed Central

Drought is the major environmental stress that limits rice productivity worldwide. In vitro somaclonal variation using different selection agents has been used for crop improvement. Here, rice plants of cv PR113 were selected in vitro on 30, 50 and 70 g L-1 polyethylene glycol 6,000 (PEG). Callus growth, proliferation, calli volume (first and second culture) and plantlet regeneration (third culture) were found to be decreased upto a certain level to acquire tolerance to PEG-induced drought. From the field data, 30 g L-1 PEG lines showed higher vegetative growth (plant height, tiller number, leaf number, shoot weight and root growth) as compared with 50 g L-1 PEG selected somaclone lines under limited irrigation. The yield parameters-panicle length, panicle weight, grains per panicle, 1,000-grain weight, grain yield per plant, harvest index and grain straw ratio were also higher in 30 g L-1 PEG lines as compared with 50 g L-1 PEG lines. The results, therefore indicate that 30 g L-1 PEG selected somaclone lines were more suited than 50 g L-1 PEG selected somaclone lines under stress as compared with WT. The finding suggests that rice cv PR113 somaclones generated on PEG are found to be drought tolerant under field condition with better yield. PMID:23333982

Verma, Dipti; Ansari, Mohammad Wahid; Agrawal, Ganesh Kumar; Rakwal, Randeep; Shukla, Alok; Tuteja, Narendra

2013-01-01

300

Mechanism of the anti-tumour effect of 2,3,5-trimethyl-6-(3-pyridylmethyl) 1,4-benzoquinone (CV-6504).  

PubMed Central

2,3,5-Trimethyl-6-(3-pyridylmethyl) 1,4-benzoquinone (CV-6504), an inhibitor of 5-lipoxygenase, effectively suppressed growth of the MAC16 tumour in vivo and prevented the accompanying cachexia, when administered daily at a dose of 10 mg kg(-1). There was a reduction in the tumour concentration of linoleic (LA), arachidonic (AA), oleic, stearic and palmitic acid. In order to elucidate the mechanism of the anti-tumour action, the effect of CV-6504 on the metabolism of AA through the 5-, 12- and 15-lipoxygenase pathways has been determined in cell lines sensitive (MAC16, MAC13, MAC26 and Caco-2) and resistant (A549 and DU-145) to CV-6504. Incubation of all cell lines with [3H]AA led to the appearance of [3H]5-, 12- and 15-HETE. Preincubation of MAC16, MAC13, MAC26 and Caco-2 with 10 microM CV-6504 inhibited the conversion of AA to 5-, 12- and 15-HETE, while in A549 and DU-145 cells there was no effect on metabolism through any lipoxygenase pathway. Two other cell lines, MDA-MB-231 and PC-3, sensitive to growth inhibition by CV-6504, are known to require LA for growth, while DU-145, which was insensitive to growth inhibition by CV-6504, showed no growth response to LA. These results suggest that some tumours are dependent on lipoxygenase metabolites of LA and AA for their continual growth, and interference with this pathway produces a specific growth inhibition. PMID:9062405

Hussey, H. J.; Tisdale, M. J.

1997-01-01

301

Safety and immunogenicity of 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Nigerian children  

PubMed Central

In a previous study, 3-dose primary vaccination of Nigerian infants with the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) was immunogenic for vaccine pneumococcal serotypes, with comparable tolerability between PHiD-CV and control groups. In an open-label study (ClinicalTrials.gov, NCT01153893), 68 primed children received a PHiD-CV booster dose co-administered with a diphtheria-tetanus-acellular pertussis (DTPa) booster dose at 15–21 months and 36 children unprimed for pneumococcal vaccination received two PHiD-CV catch-up doses (first dose co-administered with DTPa booster dose) at 15–21 and 17–23 months. Adverse events were recorded and immune responses were measured before and one month after vaccination. In both groups, pain was the most frequent solicited local symptom and fever was the most frequent solicited general symptom after the booster dose and each catch-up dose. Few grade 3 solicited symptoms and no vaccine-related serious adverse events were reported. After booster vaccination, for each vaccine serotype, at least 98.5% of children had an antibody concentration ?0.2 µg/ml and at least 94.0% had an opsonophagocytic activity (OPA) titer ?8. After 2-dose catch-up, for each vaccine serotype, at least 97.1% had an antibody concentration ?0.2 µg/ml, except for serotypes 6B (82.9%) and 23F (88.6%), and at least 91.4% had an OPA titer ?8, except for serotypes 6B (77.4%) and 19F (85.3%). PHiD-CV induced antibody responses against protein D in both groups. In conclusion, PHiD-CV administered to Nigerian toddlers as a booster dose or 2-dose catch-up was well tolerated and immunogenic for vaccine pneumococcal serotypes and protein D. PMID:24356787

Odusanya, Olumuyiwa O; Kuyinu, Yetunde A; Kehinde, Omolara A; Shafi, Fakrudeen; François, Nancy; Yarzabal, Juan Pablo; Dobbelaere, Kurt; Rüggeberg, Jens U; Borys, Dorota; Schuerman, Lode

2014-01-01

302

Identification and Expression of Two Novel Cytochrome P450 Genes, CYP6CV1 and CYP9A38, in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).  

PubMed

Cnaphalocrocis medinalis Güenée can cause severe losses in rice. Cytochrome P450s play crucial roles in the metabolism of allelochemicals in herbivorous insects. Two novel P450 cDNAs, CYP6CV1 and CYP9A38, were cloned from the midgut of C. medinalis. CYP6CV1 encodes a protein of 500 amino acid residues, while CYP9A38-predicted protein has 531 amino acid residues. Both cDNA-predicted proteins contain the conserved functional domains for all P450s. Phylogenetic analyses showed that CYP6CV1 is grouped in the cluster containing CYP6B members, while CYP9A38 is in the cluster including CYP9 members. However, both clusters are contained in the same higher lineage. Homologous analysis revealed that CYP6CV1 is most similar to CYP6B8, CYP6B7, CYP6B6, CYP6B2, and CYP6B4 with the highest amino acid identity of 41%. CYP9A38 is closest to CYP9A17, CYP9A21, CYP9A20, and CYP9A19 with the highest amino acid identity of 66%. Studies of temporal expression profiles revealed that CYP9A38 showed a steady increase in mRNA level during the five instar stages, but a low-expression level in pupae, and then presented at a high-expression level again in adults. Similar expression patterns were obtained with CYP6CV1. In the fifth instar larvae, CYP6CV1 was mainly expressed in midgut and fat bodies, whereas CYP9A38 was mainly expressed in midgut. Expression studies also revealed a 3.20-fold over-expression of CYP6CV1 and 3.54-fold over-expression of CYP9A38 after larval exposure to host rice resistance. Our results suggest that both CYP6CV1 and CYP9A38 may be involved in detoxification of rice phytochemicals. PMID:25896119

Chen, Jun; Li, Chuan; Yang, Zhifan

2015-04-01

303

The Anatomy and Bulk Composition of CAI Rims in the Vigarano (CV3) Chondrite  

NASA Astrophysics Data System (ADS)

A striking feature of Ca,Al-rich inclusions (CAIs) in chondrites is the presence of mineralogical layers that typically form rim sequences up to 50 micrometers thick [1]. Many ideas regarding the origin of CAI rims have been proposed, but none are entirely satisfactory. The detailed mineralogy and bulk compositions of relatively unaltered CAI rims in the Vigarano (CV3) chondrite described here provide constraints on hypotheses of rim formation. Rim Mineralogy: CAIs in Vigarano consist of melilite (mel)- and spinel (sp)- rich varieties, both of which are rimmed [2]. Around mel-rich objects, the layer sequence is CAI interior --> sp-rich layer (sometimes absent) --> mel/anorthite (anor) layer --> Ti-Al-rich clinopyroxene (Tpx) layer --> Al- diopside (Al-diop) layer --> olivine (ol) +/- Al-diop layer --> host matrix. The sequence around sp-rich objects differs from this in that the mel/anor layer is absent. Both the sp-rich layer around mel-cored CAIs and the cores of sp-rich CAIs in Vigarano are largely comprised of a fine-grained (<=1 micrometer) intergrowth of sp, Tpx, and minor mel and perovskite. These intergrowths are typically so fine grained that little internal texture is discernible. Mixing calculations suggest the presence of ~10 vol% Tpx in the sp-rich layer of two mel-cored CAIs, and the presence of ~35 vol% Tpx within one sp-cored CAI. The mel/anor layer is sometimes monomineralic, consisting of mel alone, or bimineralic, consisting of both mel and anor. Where bimineralic, anor typically occurs in the outer part of the layer. In places, anor (An(sub)99-100) has partially altered to nepheline and voids. Rim mel is systematically less gehlenitic than mel in the CAI interiors, especially compared to mel in the interior adjacent to the rims. The Tpx layer (>2 and up to 15 wt% TiO2) and Al-diop layer (<2 wt% TiO2) are monomineralic and show chemical zoning trends radial to the CAIs. Moving outward, TiO2 and Al2O3 generally decrease, while SiO2 and MgO increase, although Al2O3 shows a small concentration maximum in the Al-diop layer. High-quality EMPA data suggest that Ti^3+/Ti^4+ decreases outward in the Tpx layer, and that Fe^3+ is present in the Al-diop layer, implying that a steep gradient in oxidation state occurs across these two layers. The ol layer is comprised of individually zoned grains (<=5 micrometers across) that have forsteritic cores and thin (<=1 micrometer) rims of more ferrous ol. The ol grains often form triple-grain junctions and occasionally form clusters that are enclosed by Al-diop. The texture of this polycrystalline layer suggests that it formed by the attachment of preexisting ol grains onto the surfaces of CAIs, and the triple-junctions and steep zoning profiles of the ol grains suggest that they were annealed in a short-lived heating event. Bulk Rim Composition: Bulk rim compositions for several mel-rich CAIs were determined by using EMPA traverses across representative portions of the rims. These compositions plot within the sp + forsterite (fo) field of the gehlenite-anorthite-forsterite ternary diagram of Stolper [3], and are unattainable by the igneous crystallization of a mel-rich CAI composition. Moreover, a hypothetical melt with the composition of the rims has a predicted crystallization sequence (sp --> sp + fo --> sp + fo + anor or mel or Tpx) that does not correspond to observed rim sequences. It thus appears that (1) the rim region did not form through crystallization of molten CAIs; and (2) rim layers did not originate solely by the crystallization of a melt layer present on a solid CAI core [4,5]. References: [1] Wark D. A. and Lovering J. F. (1977) Proc. LSC 8th, 95-112. [2] Ruzicka A. and Boynton W. V. (1991) Meteoritics, 26, 390-391. [3] Stolper E. (1982) GCA, 46, 2159-2180. [4] Korina M. I. et al. (1982) LPS XIII, 399- 400. [5] Bunch T. E. and Chang S. (1980) Meteoritics, 15, 270- 271.

Ruzicka, A.; Boynton, W. V.

1993-07-01

304

Observation of Correlated 41Ca and 26Al in CV3 Hibonites  

NASA Astrophysics Data System (ADS)

The demonstration of the presence of ^41Ca in the early solar system, based on the observation of excess ^41K in Efremovka CAIs [1,2] has led to several attempts that sought to identify plausible stellar sites and processes that could have produced and subsequently injected a host of short-lived radionuclides(e.g. ^41Ca, ^26Al, ^60Fe, ^53Mn and ^107Pd) into the proto-solar cloud [3,4,5]. Radiogenic excess of ^26Mg (from ^26Al decay) was also found in the Efremovka CAIs where excess ^41K was present [6,7]. This association suggests possible co-production and subsequent injection of these two short-lived nuclides to the solar nebula. We have measured K and Mg isotopic composition in a single mineral phase (hibonite) having contrasting ^26Mg excesses to further substantiate this hypothesis. We have chosen hibonites from two CV3 meteorites, Efremovka and Allende for the present study. The Efremovka hibonite is from a CAI (E50) having a well-behaved Mg-Al isotopic systematics with initial ^26Al/^27Al of (4.87 +/-0.87)x10^-5 [6]. The Allende sample (HAL hibonite) on the other hand has a very low initial ^26Al/^27Al of (5.2 +/-1.7)x10^-8 [8]. We have carried out K and Mg isotopic studies of E50 hibonite and K isotopic study of HAL hibonite using procedures described previously [1,2,6]. Because of the small size of E50 hibonites, K isotopic composition could be measured in only a few hibonite grains, where the contribution from neighbouring K-rich phase(melilite) towards the measured K signal was negligible. In the case of HAL hibonite there is a large variation in K content, and the signal at ^39K is often close to the system background, while the signal at mass 41 is dominated by the [^40Ca^42Ca]6++ interference. The magnitude of this interference was estimated by measuring the [^40Ca^43Ca]^++/^43Ca^+ ratio in terrestrial (Madagascar) hibonite as well as in the HAL hibonite. We obtained a value of (7.3 +/-0.6)x10^-6 which is about four times lower than the corresponding value for pyroxene [1,2]. Definite excess of ^41K has been found in E50 hibonites with Ca/K ratio exceeding 5x10^5. A forced linear fit of the data through the solar system ^41K/^39K ratio (0.072) yields an initial ^41Ca/^40Ca of about 1.4x10^-8, which is close to the value reported for Efremovka pyroxene [1]. The Al-Mg isotopic data for the E50 hibonites reveal ^26Mg excess in them, consistent with the results reported earlier [6]. In contrast, no excess 41K was found (within the limits of our experimental uncertainties) in HAL hibonite even though its Ca/K ratio is much higher than in Efremovka hibonite. Our data provide an upper limit of 3x10^-9 for initial ^41Ca/^40Ca in HAL hibonite. These results and data obtained from studies of other Efremovka CAIs [1,2,6,7] show that : (i) ^41Ca and ^26Al are present at the same level (^41Ca/^40Ca about 1.4x10^-8 and ^26Al/^27Al about 5x10^-5) in several Efremovka CAIs and (ii) ^41Ca is very low or absent when ^26Al is at very low level (HAL hibonite). It therefore appears that ^41Ca and ^26Al are coupled either in their original stellar source(s) or were well mixed in some parcels of interstellar material before they were injected to the solar nebula. The lower initials in HAL hibonite may reflect either a heterogeneity of ^26Al and ^41Ca in the solar nebula (due to variable mixing of the source material with nebular material) or processing of material and/or redistribution of Mg and K isotopes at a later time. Although our data cannot decide between these alternatives, extensive studies of HAL and other refractory objects indicate that many CAIs evolved through multiple events in the early solar system spreading over a significant time interval [e.g. 9-12]. References: [1] Srinivasan G. et al. (1994) Astrophys. J. Lett., 431, L67-L70. [2] Srinivasan G. et al. (1995) GCA, submitted. [3] Wasserburg G. J. et al. (1995) Astrophys. J. Lett., 440, L101-L104. [4] Cameron A. G. W. et al. (1995) Astrophys. J. Lett., in press. [5] Ramaty R et al. (1995) Astrophys. J., submitted. [6] Goswami J. N. and Srinivasan G. (1994) Proc.

Sahijpal, S.; Srinivasan, G.; Wasserburg, G. J.; Goswami, J. N.

1995-09-01

305

Protection levels in vaccinated heifers with experimental vaccines Brucella abortus M1- luc and INTA 2  

Microsoft Academic Search

Brucella abortus M1-luc is a mutant strain derived from S19 vaccine strain in which most of bp26 sequence has been replaced by the luciferase coding gene. Strain I2 is a double mutant derived from M1-luc in which most of omp19 has been deleted without introduction of any genetic markers. In BALB\\/c mice, M1-luc presented equivalent performance to S19 regarding persistence,

M. A. Fiorentino; E. Campos; S. Cravero; A. Arese; F. Paolicchi; C. Campero; O. Rossetti

2008-01-01

306

Interactions between Mild NaCl Stress and Red Light during Lettuce (Lactuca sativa L. cv Grand Rapids) Seed Germination  

PubMed Central

The sensitivity of lettuce (Lactuca sativa L. cv Grand Rapids) seeds to red light was reduced by NaCl concentrations which had no effect upon the germination of continuously illuminated seeds. The germination capacity of the seeds was fully restored by increased red light exposures. Indirect evidence indicates that NaCl does not affect the photoconversion of red-absorbing form of phytochrome to the far-red absorbing form of phytochrome. Instead, the increased red light requirements are attributable to increases in the threshold levels of the far-red absorbing form of phytochrome necessary to induce germination and to changes in the slopes of the fluence-response curves. Results also show that the sensitivity of the seeds to NaCl decreased as the time between red light irradiation and the imposition of NaCl stress increased. PMID:16664360

Scorer, Keith N.; Epel, B. L.; Waisel, Yoav

1985-01-01

307

Direct identification and characterization of phenolic compounds from crude extracts of buds and internodes of grapevine (Vitis vinifera cv Merlot).  

PubMed

The crude methanol extracts of latent buds and internodes Vitis vinifera L. cv. Merlot were used for the determination of phenolic compounds by a combination of reverse phase HPLC with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). This method allowed the identification of 9 phenolic compounds without purification or fractionation. These 9 compounds were divided into three groups: procyanidins, flavonols and stilbenes. Detection by HPLC-DAD at different wave lengths of 280 nm to 320 nm, allowed the estimation of concentrations of those compounds. This method permitted, for the first time, both characterization and quantification of polyphenolic compounds in buds of grapevine. Comparison with the results obtained in internodes showed that quercetin, resveratrol tetramer and ?-viniferin had similar levels in buds and internodes while six other compounds identified had higher levels in buds. PMID:25532283

Qsaib, Said; Mateus, Nuno; Ikbal, Fatima Ez-zohra; Rifai, Lala Aicha; De Freitas, Victor; Koussa, Tayeb

2014-11-01

308

Induction of high-frequency somatic embryogenesis in geranium (Pelargonium x hortorum Bailey cv Ringo Rose) cotyledonary cultures.  

PubMed

The cv Ringo Rose of hybrid seed geranium (Pelargonium x hortorum Bailey), previously shown to be recalcitrant in culture, produced somatic embryos when cotyledonary explants were cultured on regeneration medium containing thidiazuron (TDZ), forchlorfenuron (CPPU), or a combination of indole-3-acetic acid and N(6) benzylaminopurine (IAA+BAP). Amendment of the basal medium with TDZ (0.5 ?M) was the most effective treatment. Addition of amino acids to the medium promoted the growth of somatic embryos. Retention of the proximal region of the cotyledon was crucial for regeneration, but the removal of the distal 1/3 to 1/2 cotyledon had no significant effect on somatic embryogenesis. Cotyledonary explants formed somatic embryos in higher frequency and much earlier than hypocotyl explants cultured on the same medium. The somatic embryos induced on cotyledonary explants were germinated on basal medium. More than 70% of the somatic embryos were converted into plants and transferred to soil. PMID:24178422

Murthy, B N; Singh, R P; Saxena, P K

1996-02-01

309

Spectroscopic study of impurities and associated defects in nanodiamonds from Efremovka (CV3) and Orgueil (CI) meteorites  

NASA Astrophysics Data System (ADS)

The results of spectroscopic and structural studies of phase composition and defects in nanodiamonds from Efremovka (CV3) and Orgueil (CI) chondrites indicate that nitrogen atomic environment in meteoritic nanodiamonds (MND) is similar to that observed in synthetic counterparts produced by detonation and by the Chemical Vapor Deposition (CVD)-process. Most of the nitrogen in MND appears to be confined to lattice imperfections, such as crystallite/twin boundaries and other extended defects, while the concentration of nitrogen in the MND lattice is low. It is suggested that the N-rich sub-population of MND grains may have been formed with high growth rates in environments rich in accessible N (i.e., N in atomic form or as weakly bonded compounds). For the first time the silicon-vacancy complex (the "silicon" defect) is observed in MND by photoluminescence spectroscopy.

Shiryaev, A. A.; Fisenko, A. V.; Vlasov, I. I.; Semjonova, L. F.; Nagel, P.; Schuppler, S.

2011-06-01

310

Anatalline and other methyl jasmonate-inducible nicotine alkaloids from Nicotiana tabacum cv. By-2 cell cultures.  

PubMed

Anatalline [2,4-di(3-pyridyl)piperidine] accumulation was shown to be induced by methyl jasmonate in Nicotiana tabacum cv. BY-2 cell cultures. Beside anatabine, anatalline represented the most abundant alkaloid, moreover, it was always present in two isomeric forms occurring always in similar concentrations. Both isomers could be completely separated by GC-MS. For structural analysis, the isolation of both isomers was performed using a semi-preparative HPLC system. The structures of anatalline [cis-2,4-di(3-pyridyl)piperidine] and its stereoisomer trans-2,4-di(3-pyridyl)piperidine were confirmed by MS and 1D and 2D NMR spectral data. The biosynthetic origin of anatalline was studied by feeding alkaloid precursors to BY-2 cell cultures. PMID:15490322

Häkkinen, Suvi T; Rischer, Heiko; Laakso, Into; Maaheimo, Hannu; Seppänen-Laakso, Tuulikki; Oksman-Caldentey, Kirsi-Marja

2004-10-01

311

Petrography and classification of Ca, Al-rich and olivine-rich inclusions in the Allende CV3 chondrite  

NASA Technical Reports Server (NTRS)

The results of a detailed, systematic petrographic survey of Ca, Al-rich and olivine-rich inclusions in the Allende CV3 chondrite are reported, and a new classification system based on clearly defined and readily applied petrographic criteria is presented. Most Allende inclusions are aggregates containing one or more of three distinct constituents: (1) rimmed concentric objects enriched in Al- and Ti-rich oxide minerals and various amounts of Ca-rich silicates; (2) porous, 'fine-grained' chaotic material enriched in Ca-rich silicates, especially clinopyroxenes and garnets; and (3) porous, 'fine-grained', mafic inclusion matrix, enriched in olivine, pyroxene, and feldspathoids. Two texturally distinct varieties of inclusions consist primarily of inclusion matrix: unrimmed olivine aggregates and rimmed olivine aggregates. Ca, Al-rich inclusions are classified on the basis of the size and abundance of their constituent concentric objects. Some fundamental relationships among Allende inclusions that previusly have not been emphasized are discussed.

Kormacki, A. S.; Wood, J. A.

1984-01-01

312

Al-26, Pu-244, Ti-50, REE, and trace element abundances in hibonite grains from CM and CV meteorites  

NASA Technical Reports Server (NTRS)

Hibonites from the CM meteorites Murchison, Murray, and Cold Bokkeveld, and hibonites and Ti-rich pyroxene from the CV chondrite Allende are studied. Electron microprobe measurements of major element concentrations and track and ion probe measurements of Mg and Ti isotopic ratios, rare earth elements (REEs), and trace element abundances are analyzed. Correlations between isotopic anomalies in Ti, Al-26, Pu-244, and Mg-26(asterisk) are examined. Ti isotopic anomalies are compared with REE and trace element abundance patterns. Reasons for the lack of Al-26 in the hibonites are investigated and discussed. It is observed that there is no correlation between the Ti isotopic compositions, and the presence of Mg-26(asterisk), Pu-244, and REE and trace element patterns in individual hibonite samples. The data reveal that hibonites are not interstellar dust grains but formed on a short time scale and in localized regions of the early solar system.

Fahey, A. J.; Mckeegan, K. D.; Zinner, E.; Goswami, J. N.

1987-01-01

313

Morpho-histological study of banana (Musa spp. cv. Grande Naine [AAA]) cell suspensions during cryopreservation and regeneration.  

PubMed

In this work, a morpho-histological study of banana (Musa spp. cv. Grande Naine [AAA]) embryogenic cell suspensions during cryopreservation and regeneration was performed. It was demonstrated that the regeneration process of somatic embryos originating from cryopreserved cell suspensions was different from that of control cell suspensions. Somatic embryos originating from cryopreserved cell suspensions had a unicellular origin. The regeneration process was modified not only by freezing in liquid nitrogen but also by the plasmolyzing effect of the 0.5 M sucrose solution employed during pretreatment. This result explained the high number of embryonic structures formed on M3 medium, compared with the control. Proembryos blocked at the globular stage could pursue their development when they were plated on new culture medium at a lower density after 30 days of culture on M3 medium. The unicellular origin of somatic embryos produced from cryopreserved cell suspensions offers the prospect of using cryopreservation to select non-chimeral transformed plants. PMID:20309495

Georget, Frédéric; Engelmann, Florent; Domergue, Régis; Cote, François

2009-01-01

314

Physiological and biochemical changes associated with flower development and senescence in so far unexplored Helleborus orientalis Lam. cv. Olympicus.  

PubMed

The so far unexplored H. Orientalis cv. Olympicus exhibits a unique pattern of flower senescence, involving re-greening of creamy white petaloid sepals at the later stages. The greenish sepals become photosynthetically competent immediately after pollination and persist until the seeds are set. After the seed set, the entire (green) flower abscises from the plant. Flower development of Helleborus orientalis cv. Olympicus growing in the open was divided into six stages (I-VI) from tight bud stage to the senescent stage. The average life span of an individual flower after it is fully open is about 6 days. Membrane permeability of sepal tissues estimated as electrical conductivity of leachates increased during senescence. The content of sugars and soluble proteins in the sepal tissues increased during flower opening and declined thereafter during senescence. The protease activity increased as the flower progressed towards senescence. From the present study, it becomes evident that decline in the sugar status and elevation in specific protease activity leading to degradation of proteins are the important factors regulating development and senescence in H. orientalis flowers. Although the tissue content of soluble proteins registered an overall quantitative decrease but SDS-PAGE of protein extract from sepal tissues suggested a decrease in the expression of high molecular weight proteins and an increase in low molecular weight proteins during flower development and senescence. At this stage it is not known whether the polypeptides that increased during senescence play an important role in the senescence of Helleborus orientalis flowers. The increase in these polypeptides during flower senescence is of particular interest because they may be linked to flower longevity. Understanding the nature of these proteins can provide new insights into the pathways that execute senescence and the post-transcriptional regulation of senescence in this flower system. PMID:23572993

Shahri, Waseem; Tahir, Inayatullah; Islam, Sheikh Tajamul; Bhat, Mushtaq Ahmad

2011-03-01

315

The genetics of domestication of yardlong bean, Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis  

PubMed Central

Background and Aims The genetics of domestication of yardlong bean [Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis] is of particular interest because the genome of this legume has experienced divergent domestication. Initially, cowpea was domesticated from wild cowpea in Africa; in Asia a vegetable form of cowpea, yardlong bean, subsequently evolved from cowpea. Information on the genetics of domestication-related traits would be useful for yardlong bean and cowpea breeding programmes, as well as comparative genome study among members of the genus Vigna. The objectives of this study were to identify quantitative trait loci (QTLs) for domestication-related traits in yardlong bean and compare them with previously reported QTLs in closely related Vigna. Methods Two linkage maps were developed from BC1F1 and F2 populations from the cross between yardlong bean (V. unguiculata ssp. unguiculata cv.-gr. sesquipedalis) accession JP81610 and wild cowpea (V. unguiculata ssp. unguiculata var. spontanea) accession TVnu457. Using these linkage maps, QTLs for 24 domestication-related traits were analysed and mapped. QTLs were detected for traits related to seed, pod, stem and leaf. Key Results Most traits were controlled by between one and 11 QTLs. QTLs for domestication-related traits show co-location on several narrow genomic regions on almost all linkage groups (LGs), but especially on LGs 3, 7, 8 and 11. Major QTLs for sizes of seed, pod, stem and leaf were principally located on LG7. Pleiotropy or close linkage of genes for the traits is suggested in these chromosome regions. Conclusions This is the first report of QTLs for domestication-related traits in yardlong bean. The results provide a foundation for marker-assisted selection of domestication-related QTLs in yardlong bean and enhance understanding of domestication in the genus Vigna. PMID:22419763

Kongjaimun, Alisa; Kaga, Akito; Tomooka, Norihiko; Somta, Prakit; Vaughan, Duncan A.; Srinives, Peerasak

2012-01-01

316

In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.  

PubMed

An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) ?-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana. PMID:24222501

Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert

2014-02-01

317

Whole-rock 26Al-26Mg systematics of amoeboid olivine aggregates from the oxidized CV3 carbonaceous chondrite Allende  

NASA Astrophysics Data System (ADS)

We report on mineralogy, petrography, and whole-rock 26Al-26Mg systematics of eight amoeboid olivine aggregates (AOAs) from the oxidized CV chondrite Allende. The AOAs consist of forsteritic olivine, opaque nodules, and variable amounts of Ca,Al-rich inclusions (CAIs) of different types, and show evidence for alteration to varying degrees. Melilite and anorthite are replaced by nepheline, sodalite, and grossular; spinel is enriched in FeO; opaque nodules are replaced by Fe,Ni-sulfides, ferroan olivine and Ca,Fe-rich pyroxenes; forsteritic olivine is enriched in FeO and often overgrown by ferroan olivine. The AOAs are surrounded by fine-grained, matrix-like rims composed mainly of ferroan olivine and by a discontinuous layer of Ca,Fe-rich silicates. These observations indicate that AOAs experienced in situ elemental open-system iron-alkali-halogen metasomatic alteration during which Fe, Na, Cl, and Si were introduced, whereas Ca was removed from AOAs and used to form the Ca,Fe-rich silicate rims around AOAs. The whole-rock 26Al-26Mg systematics of the Allende AOAs plot above the isochron of the whole-rock Allende CAIs with a slope of (5.23 ± 0.13) × 10-5 reported by Jacobsen et al. (2008). In contrast, whole-rock 26Al-26Mg isotope systematics of CAIs and AOAs from the reduced CV chondrite Efremovka define a single isochron with a slope of (5.25± 0.01) × 10-5 (Larsen et al. 2011). We infer that the excesses in 26Mg* present in Allende AOAs are due to their late-stage open-system metasomatic alteration. Thus, the 26Al-26Mg isotope systematics of Allende CAIs and AOAs are disturbed by parent body alteration processes, and may not be suitable for high-precision chronology of the early solar system events and processes.

Olsen, M. B.; Krot, A. N.; Larsen, K.; Paton, C.; Wielandt, D.; Schiller, M.; Bizzarro, M.

2011-11-01

318

Coventry University College is a trading name of Coventry University College Limited. A company registered in England and Wales under company number 07781274. Registered office: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB  

E-print Network

Street, Coventry, United Kingdom CV1 5FB JOB DESCRIPTION & PERSON SPECIFICATION 1. JOB INFORMATION Post company number 07781274. Registered office: Coventry University, Priory Street, Coventry, United Kingdom: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB 3. PERSON SPECIFICATION This is a new

Low, Robert

319

Effect of various Rhizobium trifolii strains on nitrogenase (C 2 H 2 ) activity profiles of red clover ( Trifolium pratense cv. Venla)  

Microsoft Academic Search

The effect of variousRhizobium trifolii strains on the nitrogenase activity profiles of red clover (Trifolium pratense cv. Venla) was studied in greenhouse and field experiments. The nitrogenase activity of nodulated roots or whole plants was measured with the acetylene reduction assay at different stages of plant growth. The mean nitrogenase activity followed plant shoot growth rate from the time when

Kristina Lindström

1984-01-01

320

USE OF REPEATED BRONCHOALVEOLAR LAVAGE IN RABBITS TO ASSESS POLLUTANT-INDUCED LUNG CHANGES IN AN ANIMAL MODEL OF CARDIOVASCULAR (CV) DISEASE.  

EPA Science Inventory

Animal models of coronary heart disease (e.g., hyperlipidemic rabbits) are being used to investigate epidemiologic associations between higher levels of air pollution and adverse CV consequences. Mechanisms by which pollutant-induced lung or systemic inflammation leads to acute C...

321

Omega Gliadin Genes Expressed in Triticum Aestivum cv Butte 86: Effects of Post-anthesis Fertilizer on Transcript Accumulation During Grain Development  

Technology Transfer Automated Retrieval System (TEKTRAN)

The partial coding sequences of omega gliadin genes expressed in developing wheat kernels Triticum aestivum cv Butte 86 were identified in EST databases. Three gene assemblies encode proteins with PQQPFP as the predominant repetitive motif. Of these, two encode proteins with at least one cysteine an...

322

Free radical scavenging activity and phenolic content in achenes and thalamus from Fragaria chiloensis ssp. chiloensis, F. vesca and F. x ananassa cv. Chandler  

Microsoft Academic Search

The total phenolic, flavonoid and anthocyanin content of achenes (true fruit) and thalamus (receptacle) from the native South American Fragaria chiloensis ssp. chiloensis (f. patagonica and f. chiloensis), Fragaria vesca and Fragaria x ananassa cv. Chandler was determined by spectrophotometric means. Highest phenolic content was found in F. vesca while lowest content was measured for white strawberry (F. chiloensis ssp.

José Cheel; Cristina Theoduloz; Jaime A. Rodríguez; Peter D. S. Caligari; Guillermo Schmeda-Hirschmann

2007-01-01

323

Effect of potassium on drought resistance of Hibiscus rosa-sinensis cv. Leprechaun: Plant growth, leaf macro- and micronutrient content and root longevity  

Microsoft Academic Search

As competition for the limited water supply available for irrigation of horticultural crops increases, research into crop management practices that enhance drought resistance, plant water-use efficiency and plant growth when water supply is limited has become increasingly essential. This experiment was conducted to determine the effect of potassium (K) nutritional status on the drought resistance of Hibiscus rosa-sinensis L. cv.

Jonathan N. Egilla; Fred T. Davies; Malcolm C. Drew

2001-01-01

324

Nickolas Themelis: Director, Earth Engineering Center http://www.seas.columbia.edu/earth/szekielda-cv.html[8/19/2009 4:40:26 PM  

E-print Network

Nickolas Themelis: Director, Earth Engineering Center http://www.seas.columbia.edu/earth/szekielda-cv.html[8/19/2009 4:40:26 PM] The Earth Engineering Center of Columbia University KARL SZEKIELDA (212) 923. Szekielda was PI in the Earth Resources Technology Satellite under Skylab (EREP). Appointed National

325

Effects of three esca-associated fungi on Vitis vinifera L.: V. Changes in the chemical and biological profile of xylem sap from diseased cv. Sangiovese vines  

Microsoft Academic Search

A vineyard of Vitis vinifera cv. Sangiovese was surveyed for incidence of esca and xylem sap collection. Sap samples were collected from healthy vines and from those with dual infection by Phaeomoniella chlamydospora (Pch) and Togninia minima (Tmi) or triple infection by Pch, Tmi and Fomitiporia mediterranea (Fme), during each early spring in a 3-year period (2001–2003). In order to

Giovanni Bruno; Lorenzo Sparapano

2007-01-01

326

Electronics Division Technical Note No. 189 File: \\\\EAGLE\\cv-cdl-sis\\Docs\\Rack\\SIS Mixer Bias Supply\\Simulation\\Report2.doc Page 1 of 5  

E-print Network

Electronics Division Technical Note No. 189 File: \\\\EAGLE\\cv-cdl-sis\\Docs\\Rack\\SIS Mixer Bias Supply\\Simulation\\Report2.doc Page 1 of 5 Stability Analysis of SIS Mixer Bias Supply with 1K Ohm Isolation Resistors 29 November 2001 J. Effland Summary This memo analyzes the stability of the CDL's SIS

Groppi, Christopher

327

Differences in Stylet Penetration Behaviors of Glassy-winged Sharpshooters on Xylella-Resistant Vitis candicans vs. Susceptible Vitis vinifera cv. ‘Chardonnay’  

Technology Transfer Automated Retrieval System (TEKTRAN)

Electrical penetration graph (EPG) monitoring was used to compare stylet penetration behaviors of glassy-winged sharpshooter (GWSS), a vector of Xylella fastidiosa (Xf), on Xf-resistant Vitis candicans grape vs. susceptible V. vinifera cv. ‘Chardonnay.’ Frequency of occurrence of X waves (represent...

328

Effects of pH, sample size, and solvent partitioning on recovery of soluble phenolic acids and isoflavonoids in red clover (Trifolium pratense cv. Kenland)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Several extraction parameters were tested to determine optimal conditions for extracting phenolics from red clover (Trifolium pratense L. cv. Kenland). HPLC-UV profiles were compared before and after partitioning a methanolic extract of soluble phenolics with ethyl acetate-ether (1:1, v/v). The e...

329

CHANGES IN THE STRATIFICATION AND SPATIAL DISTRIBUTION OF THE BANANA (MUSA AAA CV. GRAND NAINE) ROOT SYSTEM OF POOR, REGULAR, AND GOOD DEVELOPED PLANTS  

Microsoft Academic Search

In an attempt to explain the difference in banana (Musa AAA cv. Grand Naine) plants with poor, regular, and good development, their root stratification and spatial distribution was determined in Costa Rican field conditions. Samples were taken from soil blocks at 0 to 30, 30 to 60, and 60 to 90 cm distances from the pseudostem, and at every 15

M. Araya; F. Blanco

2001-01-01

330

Survey of 42,000 Gossypium hirsutum cv. Maxxa BAC-End Sequences and Frequency, Type, and Annotation of BAC-derived SSRs.  

Technology Transfer Automated Retrieval System (TEKTRAN)

The quest for more molecular markers is a major initiative in cotton, which lags behind crops such as soybean, maize, and rice in this type of research. In an effort to increase the number of microsatellite markers in Gossypium, BAC-end sequences from a publicly available Gossypium hirsutum cv. Maxx...

331

Draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi).  

PubMed

Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

Kolton, Max; Green, Stefan J; Harel, Yael Meller; Sela, Noa; Elad, Yigal; Cytryn, Eddie

2012-10-01

332

A large plasmid isolated from Rhizobium huakuii bv. renge that includes genes for both nodulation of Astragalus sinicus cv. Japan and nitrogen fixation  

Microsoft Academic Search

Rhizobium huakuii bv. renge, which is able to produce nodules on Astragalus sinicus cv. Japan, has been assigned to a new biovariety based on its host range and taxonomic characteristics. We reproducibly demonstrated the presence of an indigenous large plasmid, with a molecular mass of about 260 Mda, in R. huakuii bv. renge and named the plasmid pRhYM. Using a

Yong Xu; Yoshikatsu Murooka

1995-01-01

333

A new immuno- dystrophin-deficient model, the NSG-mdx4Cv mouse, provides evidence for functional improvement following allogeneic satellite cell transplantation  

PubMed Central

Transplantation of a myogenic cell population into an immunodeficient recipient is an excellent way of assessing the in vivo muscle-generating capacity of that cell population. To facilitate both allogeneic and xenogeneic transplantations of muscle-forming cells in mice we have developed a novel immunodeficient muscular dystrophy model, the NSG-mdx4Cv mouse. The IL2Rg mutation, which is linked to the Dmd gene on the X chromosome, simultaneously depletes NK cells and suppresses thymic lymphomas, issues that limit the utility of the SCID/mdx model. The NSG-mdx4Cv mouse presents a muscular dystrophy of similar severity to the conventional mdx mouse. We show that this animal supports robust engraftment of both pig and dog muscle mononuclear cells. The question of whether satellite cells prospectively isolated by flow cytometry can confer a functional benefit upon transplantation has been controversial. Using allogeneic Pax7-ZsGreen donors and NSG-mdx4Cv recipients, we demonstrate definitively that as few as 900 FACS-isolated satellite cells can provide functional regeneration in vivo, in the form of an increased mean maximal force-generation capacity in cell-transplanted muscles, compared to a sham-injected control group. These studies highlight the potency of satellite cells to improve muscle function, and the utility of the NSG-mdx4Cv model for studies on muscle regeneration and Duchenne muscular dystrophy therapy. PMID:23606600

Arpke, Robert W.; Darabi, Radbod; Mader, Tara L.; Zhang, Yu; Toyama, Akira; Lonetree, Cara-lin; Nash, Nardina; Lowe, Dawn A.; Perlingeiro, Rita C.R.; Kyba, Michael

2013-01-01

334

arXiv:0807.1594v1[math.CV]10Jul2008 EVOLUTION FAMILIES AND THE LOEWNER EQUATION I: THE  

E-print Network

arXiv:0807.1594v1[math.CV]10Jul2008 EVOLUTION FAMILIES AND THE LOEWNER EQUATION I: THE UNIT DISC a general version of the Loewner differential equation which allows us to present a new and unified correspondence with solutions to this new type of Loewner equations. Also, we give a Berkson-Porta type formula

Contreras, Manuel D.

335

A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV Hollot2, J Horowitz3, and RP Shrestha4, Y Chait5  

E-print Network

A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV on performance of anemia management protocols. · Anemia of end-stage kidney disease (ESRD) is characterized-specific gains should play a role in their design. · The interaction of anemia management protocols (AMPs

Massachusetts at Amherst, University of

336

Feifei Pan CV--Last updated on October 22, 2014 Page 1 of 10 Department of Geography, University of North Texas, Denton, TX 76203  

E-print Network

., Zhang D., Yang N., Pan Z., and Wei Y., 2014: Spatial analysis of climate change in Inner Mongolia during/215 in Environmental Sciences, G=0]. [p] Nie S., Wang C., Li G., Pan F., Xi X., and Luo S., 2014: A signal to noiseFeifei Pan CV--Last updated on October 22, 2014 Page 1 of 10 Feifei Pan Department of Geography

Pan, Feifei

337

Mutation breeding of banana cv. Highgate ( Musa spp., AAA Group) for tolerance to Fusarium oxysporum f. sp. cubense using chemical mutagens  

Microsoft Academic Search

Shoot apices of in vitro-grown cultures of banana (Musa spp., AAA Group cv. Highgate) were treated with various concentrations of the mutagens sodium azide, diethyl sulphate, and ethyl methanesulphonate to evaluate their effectiveness in inducing mutations and also with the aim of producing variants tolerant to the fungus Fusarium oxysporum f. sp. cubense. This fungus causes fusarial wilt or Panama

B. Bhagwat; E. J. Duncan

1998-01-01

338

A new immuno-, dystrophin-deficient model, the NSG-mdx(4Cv) mouse, provides evidence for functional improvement following allogeneic satellite cell transplantation.  

PubMed

Transplantation of a myogenic cell population into an immunodeficient recipient is an excellent way of assessing the in vivo muscle-generating capacity of that cell population. To facilitate both allogeneic and xenogeneic transplantations of muscle-forming cells in mice, we have developed a novel immunodeficient muscular dystrophy model, the NSG-mdx(4Cv) mouse. The IL2Rg mutation, which is linked to the Dmd gene on the X chromosome, simultaneously depletes NK cells and suppresses thymic lymphomas, issues that limit the utility of the SCID/mdx model. The NSG-mdx(4Cv) mouse presents a muscular dystrophy of similar severity to the conventional mdx mouse. We show that this animal supports robust engraftment of both pig and dog muscle mononuclear cells. The question of whether satellite cells prospectively isolated by flow cytometry can confer a functional benefit upon transplantation has been controversial. Using allogeneic Pax7-ZsGreen donors and NSG-mdx(4Cv) recipients, we demonstrate definitively that as few as 900 FACS-isolated satellite cells can provide functional regeneration in vivo, in the form of an increased mean maximal force-generation capacity in cell-transplanted muscles, compared to a sham-injected control group. These studies highlight the potency of satellite cells to improve muscle function and the utility of the NSG-mdx(4Cv) model for studies on muscle regeneration and Duchenne muscular dystrophy therapy. PMID:23606600

Arpke, Robert W; Darabi, Radbod; Mader, Tara L; Zhang, Yu; Toyama, Akira; Lonetree, Cara-Lin; Nash, Nardina; Lowe, Dawn A; Perlingeiro, Rita C R; Kyba, Michael

2013-08-01

339

Physical Analysis of the Complex Rye (Secale cereale L.) Alt4 Aluminium (Aluminum) Tolerance Locus Using a Whole-Genome BAC Library of Rye cv. Blanco  

Technology Transfer Automated Retrieval System (TEKTRAN)

Rye is a diploid crop species with many outstanding qualities, and is also important as a source of new traits for wheat and triticale improvement. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies. The library provides a 6 × genome ...

340

L'aptitude au croisement avec le seigle (Secale cereale L.) d'une ligne hexaplode de Triticum (cv. Roazon rsul-  

E-print Network

-clés additionnels : Blé, Triticum aestivum, Trsticale, Gènes d'incompatibilité, lntrogression. SUMMARY Crossability. Additional key-words : Wheat, Triticum aestivum, Triticale, Cross-compatibility, Crossability genesL'aptitude au croisement avec le seigle (Secale cereale L.) d'une lignée hexaploïde de Triticum (cv

Paris-Sud XI, Université de

341

[Effects of idebenone (CV-2619) on neurological deficits, local cerebral blood flow, and energy metabolism in rats with experimental cerebral ischemia].  

PubMed

Improvement of energy metabolism in ischemic cerebral tissue benefits the therapy of occlusive cerebrovascular lesions. In the present study, the effects of 6-(10-hydroxydecyl)-2, 3-dimethoxy-5-methyl-1, 4-benzoquinone (idebenone, CV-2619) on neurological signs, local cerebral blood flow, and cerebral energy metabolism were assessed in stroke-prone spontaneously hypertensive rats (SHRSP) with bilateral carotid artery occlusion (BCAO). Pretreatment with CV-2619 (10-100 mg/kg, p.o.) for three or ten successive days delayed the onset of ischemic seizure (acute stroke) and prolonged survival time in the SHRSP. When the compound (100 mg/kg, i.p.) was given once 30 min after BCAO, it exerted similar ameliorating effects on the neurological deficits. When CV-2619 (100 mg/kg for 3 days) was given orally, it did not inhibit a decrease in regional cerebral blood flow induced by the carotid artery occlusion. However, the same treatment markedly inhibited increases in lactate content and lactate/pyruvate ratio and a decrease in ATP content in the cerebral cortex. In addition, the compound showed no effect on cerebral blood flow in normal rats. These results suggest that CV-2619 has an ameliorating effect on neurological deficits related with cerebral ischemia, and this effect is mediated by improved cerebral energy metabolism. PMID:6500404

Nagaoka, A; Suno, M; Shibota, M; Kakihana, M

1984-09-01

342

Chemical Composition of the Essential Oil Isolated from Wild Catnip Nepeta cataria L. cv. citriodora from the Drôme Region of France  

Microsoft Academic Search

Nepeta cataria L. cv. citriodora growing wild in the Drôme region of France was brought into cultivation. Oils produced from cultivated plants harvested throughout the growing season were analyzed by GC and GC\\/MS. Although 42 components were identified, the oil composition did not depend on the time of harvesting or storage of the plant material prior to distillation. The oil

Jean-Claude Chalchat; Jacques Lamy

1997-01-01

343

Long-Term Fungal Inhibitory Activity of Water-Soluble Extracts of Phaseolus vulgaris cv. Pinto and Sourdough Lactic Acid Bacteria during Bread Storage?  

PubMed Central

The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts of L. brevis AM7 sourdough and P. vulgaris cv. Pinto had a MIC of 40 mg of peptide/ml and 30.9 mg of protein/ml, respectively. MICs were markedly lower when chemically synthesized peptides or partially purified protein fractions were used. The water-soluble extract of P. vulgaris cv. Pinto showed inhibition toward a large number of fungal species isolated from bakeries. Phaseolin alpha-type precursor, phaseolin, and erythroagglutinating phytohemagglutinin precursor were identified in the water-soluble extract of P. vulgaris cv. Pinto by nano liquid chromatography-electrospray ionization-tandem mass spectrometry. When the antifungal activity was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all three proteins were inhibitory. A mixture of eight peptides was identified from the water-soluble extract of sourdough L. brevis AM7, and five of these exhibited inhibitory activity. Bread was made at the pilot plant scale by sourdough fermentation with L. brevis AM7 and addition of the water-soluble extract (27%, vol/wt; 5 mg of protein/ml) of P. vulgaris cv. Pinto. Slices of bread packed in polyethylene bags did not show contamination by fungi until at least 21 days of storage at room temperature, a level of protection comparable to that afforded by 0.3% (wt/wt) calcium propionate. PMID:18849463

Coda, Rossana; Rizzello, Carlo G.; Nigro, Franco; De Angelis, Maria; Arnault, Philip; Gobbetti, Marco

2008-01-01

344

Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability  

PubMed Central

Background Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation. Reviewer This article was reviewed by Sándor Pongor, L. Aravind and Anthony M. Poole. PMID:23324115

2013-01-01

345

Transport of Ascorbic and Dehydroascorbic Acids across Protoplast and Vacuole Membranes Isolated from Barley (Hordeum vulgare L. cv Gerbel) Leaves.  

PubMed Central

Protoplasts, vacuoles, and chloroplasts were isolated from leaves of 8-d-old barley (Hordeum vulgare L. cv Gerbel) seedlings. Transport of ascorbate and dehydroascorbate into protoplasts and vacuoles was investigated. Contents of ascorbic acid, glutathione, and [alpha]-tocopherol and ascorbate peroxidase activity and glutathione reductase activity were analyzed in protoplasts, vacuoles, and chloroplasts. Uptake of ascorbate and dehydroascorbate by protoplasts showed saturation kinetics (Km = 90 [mu]M reduced ascorbic acid, 20 [mu]M dyhydroascorbic acid). Effects of various membrane transport inhibitors suggested that transport was carrier mediated and driven by a proton electrochemical gradient. Translocation of ascorbate and dehydroascorbate into vacuoles did not show saturation kinetics. Neither was it influenced by effectors or by ATP but only by Mg2+, suggesting that translocation did not occur by carrier. Ascorbic acid was predominantly localized in the cytosol. Contents in the chloroplasts and vacuoles were low. The results are consistent with the view that ascorbate is synthesized in the cytosol and released to chloroplasts, apoplast, and vacuole following a concentration gradient. Translocation from the apoplast into the cytosol is against a steep gradient and appears to control the concentration of ascorbic acid in the apoplast. In its function as an antioxidant, ascorbate in the apoplast may be oxidized to dehydroascorbate, which can be efficiently transported back into the cytosol for regeneration to ascorbate. PMID:12232318

Rautenkranz, AAF.; Li, L.; Machler, F.; Martinoia, E.; Oertli, J. J.

1994-01-01

346

Characterisation of extra virgin olive oils from Galician autochthonous varieties and their co-crushings with Arbequina and Picual cv.  

PubMed

The current trend of the olive oil market is the production of high quality extra from traditional minor olive varieties with peculiar and differentiated characteristics (especially with respect to the aromatic and phenolic composition). In this way, the interest of Galician oil producers (NW Spain) in recovering old autochthonous Local olive fruits has increased substantially in recent years. In order to investigate the potential of the Local olives by either producing high quality monovarietal oils or mixing with the most widespread olives in Galicia (Arbequina and Picual cv.), quality indices, and fatty acid composition as well as volatile and phenolic profiles were determined and compared. All EVOOs studied in this work can be considered as "extra virgin olive oil" due to quality indices fell within the ranges established in legislation. Picual and Local olive oils as well as those resulting from their co-crushing reach values which are required by EU legislation to add the specific health claim on the oil label. Co-crushing Picual:Local (80:20) provided a significant enhancement of grass and apple nuances and a decrease of banana notes with respect to Picual oils. The co-crushing process improved sensory and health properties of Picual extra virgin olive oils. The effect of co-crushing on phenolics, ester volatiles and banana nuances cannot be easily modulated, contrary to quality indices and fatty acid composition, both changing linearly in strict correlation with the fruit mass ratio. PMID:25624261

Reboredo-Rodríguez, P; González-Barreiro, C; Cancho-Grande, B; Fregapane, G; Salvador, M D; Simal-Gándara, J

2015-06-01

347

Response of different maturity stages of sapota (Manilkara achras Mill.) cv. Kallipatti to in-package ethylene absorbent.  

PubMed

Sapota fruits are highly perishable due to their climacteric nature. The rapid softening of fruits is primarily due to high activity of many oxidative enzymes and liberation of ethylene. Harvest maturity plays a crucial role in deciding the marketability of climacteric fruits in general. Attempt has been made to evaluate the response of ethylene absorbent on variable maturity groups of harvested Sapota cv. Kallipatti with the objective to delay the ripening during transit and extend its marketability during storage at ambient condition (27-32 °C & 65-75% R.H.). Harvested fruits having three different degree of ripeness (as maturity indices viz. mature, half-ripe and ripe) were packed with or without ethylene absorbent sachets (Bioconservación, France) in 10 kg CFB boxes and transported from Dahnu to Delhi covering a distant of approximately 2500 KM by truck on road along with conventional packaging as control. The fruits were evaluated immediately on arrival at Delhi and subsequently during storage for various physical, physiological, biochemical and decay parameters. Mature fruits with ethylene absorbent exhibited maximum delay in ripening, low ethylene liberation, weight loss and high fruit firmness. The response of ethylene absorbent to extend the marketability of ripe fruit was not significant. PMID:23572819

Bhutia, Wangdup; Pal, R K; Sen, Sangita; Jha, S K

2011-12-01

348

Intake and Performance of Yearling Steers Grazing Guineagrass (Panicum maximum cv. Tanzânia) Pasture Supplemented with Different Energy Sources  

PubMed Central

The aim of this study was to evaluate the efficiency of supplements containing different energy sources in relation to mineral supplementation of steers grazing guineagrass (Panicum maximum cv Tanzânia) pasture, during the dry season. The experimental design was a randomized block with three treatments and four replications. The treatments consisted of a mineral supplementation and two other supplements, one based on corn seed and the other based on soybean hulls, and provided at 0.8% of body weight. Forty-eight, 12 month-old crossbred steers with an average initial body weight of 267 kg, were assigned to twelve paddocks (1,125 ha) of guineagrass. The animals that were fed with soybean hulls and corn seed presented a greater average daily gain (0.982 and 0.937) when compared with the mineral supplementation. Soybean hulls can be used as a satisfactory food source, replacing corn as an energy source in the supplementation of beef cattle without compromising animal performance. PMID:25049797

Santana, M. C. A.; Euclides, V. B. P.; Mancio, A. B.; Medeiros, S. R.; Costa, J. A. R.; Oliveira, R. L.

2013-01-01

349

5S program to reduce change-over time on forming department (case study on CV Piranti Works temanggung)  

NASA Astrophysics Data System (ADS)

Productivity is one aspect that determines the success of a company in the competitive world of business. There are seven main types of activities that do not have value-added in manufacturing processes such as overproduction, waiting time, transportation, excess inventory, unnecessary motion and defects. The whole activity is a waste (waste) that can cause harm to the Company. Therefore, in production activities is important to pay attention so that the objectives of production productivity can be achieved. Problems experienced by CV Piranti Works is a production target is not achieved resulting in a lost sale raises the cost of which can cause harm to the Company. From the analysis conducted major known cause of the problem is the length of time required for changeover. This is supported by the high non-value added activity in the changeover activities. Lean Manufacturing is an approach to make system more efficient by reducing waste. This study refers to the book compiled by Takashi Osada (2004) and several other references. In this research used method 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) for the of forming departement. The purpose of this research is to design a work environment using the 5S method (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) and make arrangement of equipment and working tool cabinet design with TRIZ methods. From these results, is expected to eliminate or reduce of non-value added activity and improved the changeover time so as to meet production targets completion of the company.

Rosiana Dewi, Septika; Setiawan, Budi; P, Susatyo Nugroho W.

2013-06-01

350

Characterization of the adaptive response of grapevine (cv. Tempranillo) to UV-B radiation under water deficit conditions.  

PubMed

This work aims to characterize the physiological response of grapevine (Vitis vinifera L.) cv. Tempranillo to UV-B radiation under water deficit conditions. Grapevine fruit-bearing cuttings were exposed to three levels of supplemental biologically effective UV-B radiation (0, 5.98 and 9.66kJm(-2)day(-1)) and two water regimes (well watered and water deficit), in a factorial design, from fruit-set to maturity under glasshouse-controlled conditions. UV-B induced a transient decrease in net photosynthesis (Anet), actual and maximum potential efficiency of photosystem II, particularly on well watered plants. Methanol extractable UV-B absorbing compounds (MEUVAC) concentration and superoxide dismutase activity increased with UV-B. Water deficit effected decrease in Anet and stomatal conductance, and did not change non-photochemical quenching and the de-epoxidation state of xanthophylls, dark respiration and photorespiration being alternative ways to dissipate the excess of energy. Little interactive effects between UV-B and drought were detected on photosynthesis performance, where the impact of UV-B was overshadowed by the effects of water deficit. Grape berry ripening was strongly delayed when UV-B and water deficit were applied in combination. In summary, deficit irrigation did not modify the adaptive response of grapevine to UV-B, through the accumulation of MEUVAC. However, combined treatments caused additive effects on berry ripening. PMID:25617319

Martínez-Lüscher, J; Morales, F; Delrot, S; Sánchez-Díaz, M; Gomès, E; Aguirreolea, J; Pascual, I

2015-03-01

351

Effects of elicitors on the production of resveratrol and viniferins in cell cultures of Vitis vinifera L. cv Italia.  

PubMed

Methyl jasmonate, jasmonic acid and chitosan were tested as elicitors on cell suspension cultures obtained from Vitis vinifera cv Italia to investigate their effect on stilbene production. Stilbene accumulation in the callus, grown under nonelicited conditions, was also investigated. Calli and cell suspensions were obtained in a B5 culture medium supplemented with 0.2 mg L(-1) NAA and 1 mg L(-1) KIN. Stilbene determination was achieved by HPLC/DAD/MS. Whereas callus biosynthesized only piceid, cell suspensions elicited with jasmonates produced several stilbenes, mainly viniferins. In suspended cells, methyl jasmonate and jasmonic acid were the most effective in stimulating stilbene biosynthesis, whereas chitosan was less effective; in fact, the amount of stilbenes obtained with this elicitor was not significantly different from that obtained for the control cells. The maximum production of total stilbenes was at day 20 of culture with 0.970 and 1.023 mg g(-1) DW for MeJA and JA, respectively. PMID:21751812

Santamaria, Anna Rita; Mulinacci, Nadia; Valletta, Alessio; Innocenti, Marzia; Pasqua, Gabriella

2011-09-14

352

James Webb Space Telescope (JWST) Integrated Science Instruments Module (ISIM) Cryo-Vacuum (CV) Test at GSFC  

NASA Technical Reports Server (NTRS)

JWST ISIM has entered into its system-level testing program at NASA Goddard Space Flight Center (GSFC). In December 2013, ISIM successfully completed the first in a series of three cryo-vacuum tests, which included two flight science instruments. Since then, there have been full-fledged efforts towards the CV2 test scheduled to finish at the end of 2014. The complexity of the mission has generated challenging requirements that demand highly reliable system performance and capabilities from the Space Environment Simulator (SES) vacuum chamber. In order to satisfy the program requirements, GSFC had to develop unique structural and thermal hardware to test ISIM. Most noteworthy is a helium shroud structure and cooling system built in order to achieve operational temperatures below 20K (-253C). This paper: (1) provides an overview of the integrated mechanical and thermal facility systems required to achieve the objectives of JWST ISIM testing, (2) communicates the performance and challenges of the SES during the first ISIM test, and (3) summarizes the action plan to improve the system prior to the next test.

Yew, Calinda M.

2014-01-01

353

Intake and Performance of Yearling Steers Grazing Guineagrass (Panicum maximum cv. Tanzânia) Pasture Supplemented with Different Energy Sources.  

PubMed

The aim of this study was to evaluate the efficiency of supplements containing different energy sources in relation to mineral supplementation of steers grazing guineagrass (Panicum maximum cv Tanzânia) pasture, during the dry season. The experimental design was a randomized block with three treatments and four replications. The treatments consisted of a mineral supplementation and two other supplements, one based on corn seed and the other based on soybean hulls, and provided at 0.8% of body weight. Forty-eight, 12 month-old crossbred steers with an average initial body weight of 267 kg, were assigned to twelve paddocks (1,125 ha) of guineagrass. The animals that were fed with soybean hulls and corn seed presented a greater average daily gain (0.982 and 0.937) when compared with the mineral supplementation. Soybean hulls can be used as a satisfactory food source, replacing corn as an energy source in the supplementation of beef cattle without compromising animal performance. PMID:25049797

Santana, M C A; Euclides, V B P; Mancio, A B; Medeiros, S R; Costa, J A R; Oliveira, R L

2013-03-01

354

Dehydropyrrolizidine Alkaloids, Including Monoesters with an Unusual Esterifying Acid, from Cultivated Crotalaria juncea (Sunn Hemp cv.’Tropic Sun’)  

PubMed Central

Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves and seeds of ‘Tropic Sun’ were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid content of the roots, stems, leaves of immature plants was estimated to be 0.05, 0.12 and 0.01% w/w respectively while seeds were estimated to contain 0.15% w/w. PMID:22429238

Colegate, Steven M.; Gardner, Dale R.; Joy, Robert J.; Betz, Joseph M.; Panter, Kip E.

2012-01-01

355

Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening  

PubMed Central

Background Cluster thinning is an agronomic practice in which a proportion of berry clusters are removed from the vine to increase the source/sink ratio and improve the quality of the remaining berries. Until now no transcriptomic data have been reported describing the mechanisms that underlie the agronomic and biochemical effects of thinning. Results We profiled the transcriptome of Vitis vinifera cv. Sangiovese berries before and after thinning at veraison using a genome-wide microarray representing all grapevine genes listed in the latest V1 gene prediction. Thinning increased the source/sink ratio from 0.6 to 1.2 m2 leaf area per kg of berries and boosted the sugar and anthocyanin content at harvest. Extensive transcriptome remodeling was observed in thinned vines 2 weeks after thinning and at ripening. This included the enhanced modulation of genes that are normally regulated during berry development and the induction of a large set of genes that are not usually expressed. Conclusion Cluster thinning has a profound effect on several important cellular processes and metabolic pathways including carbohydrate metabolism and the synthesis and transport of secondary products. The integrated agronomic, biochemical and transcriptomic data revealed that the positive impact of cluster thinning on final berry composition reflects a much more complex outcome than simply enhancing the normal ripening process. PMID:22192855

2011-01-01

356

Effect of salicylhydroxamic acid on endosperm strength and embryo growth of Lactuca sativa L. cv Waldmann's Green seeds  

NASA Technical Reports Server (NTRS)

Salicylhydroxamic acid (SHAM) stimulated germination of photosensitive lettuce (Lactuca sativa L. cv Waldmann's Green) seeds in darkness. To determine whether SHAM acts on the embryo or the endosperm, we investigated separately effects of SHAM on growth potential of isolated embryos as well as on endosperm strength. Embryo growth potential was quantified by incubating decoated embryos in various concentrations of osmoticum and measuring subsequent radicle elongation. Growth potential of embryos isolated from seeds pretreated with 4 millimolar SHAM was equal to that of untreated controls. Rupture strength of endosperm tissue excised from seeds pretreated with SHAM was 33% less than that of controls in the micropylar region. To determine if the embryo must be in contact with the endosperm of SHAM to weaken the endosperm, some endosperms were incubated with SHAM only after dissection from seeds. Rupture strength of SHAM-treated, isolated endosperms in the micropylar region was 25% less than that of untreated controls. There was no difference in rupture strength in the cotyledonary region of endosperm isolated from seeds treated with SHAM in buffer or buffer alone. SHAM therefore stimulates germination not by enhancing embryo growth potential, but by weakening the micropylar region of the endosperm enclosing the embryo.

Brooks, C. A.; Mitchell, C. A.

1988-01-01

357

Profiles of phenolic compounds and purine alkaloids during the development of seeds of Theobroma cacao cv. Trinitario.  

PubMed

Changes occurring in phenolic compounds and purine alkaloids, during the growth of seeds of cacao (Theobroma cacao) cv. Trinitario, were investigated using HPLC-MS/MS. Extracts of seeds with a fresh weight of 125, 700, 1550, and 2050 mg (stages 1-4, respectively) were analyzed. The phenolic compounds present in highest concentrations in developing and mature seeds (stages 3 and 4) were flavonols and flavan-3-ols. Flavan-3-ols existed as monomers of epicatechin and catechin and as procyanidins. Type B procyanidins were major components and varied from dimers to pentadecamer. Two anthocyanins, cyanidin-3-O-arabinoside and cyanidin-3-O-galactoside, along with the N-phenylpropernoyl-l-amino acids, N-caffeoyl-l-aspartate, N-coumaroyl-l-aspartate, N-coumaroyl-3-hydroxytyrosine (clovamide), and N-coumaroyltyrosine (deoxyclovamide), and the purine alkaloids theobromine and caffeine, were present in stage 3 and 4 seeds. Other purine alkaloids, such as theophylline and additional methylxanthines, did not occur in detectable quantities. Flavan-3-ols were the only components to accumulate in detectable quantities in young seeds at developmental stages 1 and 2. PMID:23215441

Pereira-Caro, Gema; Borges, Gina; Nagai, Chifumi; Jackson, Mel C; Yokota, Takao; Crozier, Alan; Ashihara, Hiroshi

2013-01-16

358

Spectral variability of /s/ in sV and sCV sequences produced by adults and children  

NASA Astrophysics Data System (ADS)

Previous research has demonstrated that both children and adults produce /s/ with greater spectral variability in /sp/ sequences than /st/ sequences, when these sequences are embedded in the medial position of CVCCVC nonwords [B. Munson, J. Acoust. Soc. Am. 110, 1203-1206 (2001)]. The current study examined whether this result could be replicated when /s/ is embedded in syllable-onset clusters, with a variety of following consonants and vowels. Adults and children aged 3-7 were recorded producing multiple tokens of sV and sCV nonwords, where the vowel was either /i/, /opena/, or /u/, and the consonant was either /p/, /t/, /w/, or /l/. For each token, the spectral mean of non-overlapping 10-ms windows of frication noise was calculated. Nonlinear regressions of the form y=aebx were used to predict the spectral mean of each portion of frication noise from its position in the fricative. The resulting measure of model fit, R2, was used as an index of within-speaker variability. For each participant, separate R2 values were calculated for /s/ in each of the 15 phonetic contexts. Analyses will address the influence of age, consonant context, and vowel context on spectral variability.

Munson, Benjamin

2002-05-01

359

Some observations of the effects of applied magnetic fields on growth of Avena coleoptiles. [Avena sativa cv. Garry  

SciTech Connect

Applied magnetic fields (5000-18,000 Gauss) disrupt the pattern of cellulose microfibril deposition in cells of Avena sativa cv. Garry coleoptiles. Freeze fracture reveals the microfibrils of the innermost wall layers to be randomly oriented, rather than parallel and transverse to the elongation axis as seen in control plants. Whole plants exposed to the magnetic field at any field intensity tested (5000-18,000 Gauss) displayed inhibited elongation growth. After two hours in a 5000 G field, plants show some recovery of elongation growth while maintained in the field, with elongation rates of 0.2 + 0.06 mm/hr as compared to control plants with 0.9 + 0.12 mm/hr elongation. Avena seeds which were germinated directly between pole pieces of a 5000 Gauss magnet produced slow-growing seedlings whose roots and coleoptiles grew toward opposite magnetic poles. Audus (1960) coined the phrase magnetotropism to describe a similar phenomenon in pollen tubes growing in magnetic fields.

Brown, D.S.; Gretz, M.; Brown, R.M. Jr.

1986-04-01

360

Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection.  

PubMed

Verticillium wilt of cotton (Gossypium hirsutum) is a widespread and destructive disease that is caused by the soil-borne fungus pathogen Verticillium dahliae (V. dahliae). To study the molecular mechanism in wilt tolerance, suppression subtractive hybridization (SSH) and dot blot techniques were used to identify the specifically expressed genes in a superior wilt-resistant cotton cultivar (G. hirsutum cv. Zhongzhimian KV1) after inoculation with pathogen. cDNAs from the root tissues of Zhongzhimian KV1 inoculated with V. dahliae strain V991 or water mock were used to construct the libraries that contain 4800 clones. Based on the results from dot blot analysis, 147 clones were clearly induced by V. dahliae and selected from the SSH libraries for sequencing. A total of 92 up-regulated and 7 down-regulated non-redundant expressed sequences tags (ESTs) were identified as disease responsive genes and classified into 9 functional groups. Two important clues regarding wilt-resistant G. hirsutum were obtained from this study. One was Bet v 1 family; the other was UbI gene family that may play an important role in the defense reaction against Verticillium wilt. The result from real-time quantitative reverse transcription polymerase chain reaction showed that these genes were activated quickly and transiently after inoculation with V. dahliae. PMID:22733494

Zhang, Wen-Wei; Jian, Gui-Liang; Jiang, Teng-Fei; Wang, Sheng-Zheng; Qi, Fang-Jun; Xu, Shi-Chang

2012-10-01

361

Molecular cloning and expression of a gene encoding alcohol acyltransferase (MdAAT2) from apple (cv. Golden Delicious).  

PubMed

Volatile esters are major aroma components of apple, and an alcohol acyltransferase (AAT) catalyzes the final step in ester biosynthesis. The gene MdAAT2, which encodes a predicted 51.2 kDa protein containing features of other acyl transferases, was isolated from Malus domestica Borkh. (cv. Golden Delicious). In contrast to other apple varieties, the MdAAT2 gene of Golden Delicious is exclusively expressed in the fruit. The MdAAT2 protein is about 47.9 kDa and mainly localized in the fruit peel, as indicated by immunoblot and immunolocalization analysis. Northern blot and immunoblot analysis showed that the transcription and translation of MdAAT2 have a positive correlation with apple AAT enzyme activity and ester production, except in the later ripening stage, suggesting that MdAAT2 is involved in the regulation of ester biosysthesis and that a post-translation modification may be involved in regulation of AAT enzyme activity. Tissue disk assays of fruit peel revealed that using extraneous alcohols can recover the corresponding ester formation. Transcription and translation of MdAAT2 were both depressed by 1-methylcyclopropene (1-MCP) treatment and subsequent ester production was also prevented. These results suggest that: (1) ester production is mainly regulated by MdAAT2; (2) ethylene is also involved in this regulatory progress and (3) ester compounds rely principally on the availability of substrates. PMID:16524607

Li, Dapeng; Xu, Yunfeng; Xu, Gangming; Gu, Lingkun; Li, Dequan; Shu, Huairui

2006-04-01

362

Nitrogen balance for wheat canopies (Triticum aestivum cv. Veery 10) grown under elevated and ambient CO2 concentrations  

NASA Technical Reports Server (NTRS)

We examined the hypothesis that elevated CO2 concentration would increase NO3- absorption and assimilation using intact wheat canopies (Triticum aestivum cv. Veery 10). Nitrate consumption, the sum of plant absorption and nitrogen loss, was continuously monitored for 23 d following germination under two CO2 concentrations (360 and 1000 micromol mol-1 CO2) and two root zone NO3- concentrations (100 and 1000 mmol m3 NO3-). The plants were grown at high density (1780 m-2) in a 28 m3 controlled environment chamber using solution culture techniques. Wheat responded to 1000 micromol mol-1 CO2 by increasing carbon allocation to root biomass production. Elevated CO2 also increased root zone NO3- consumption, but most of this increase did not result in higher biomass nitrogen. Rather, nitrogen loss accounted for the greatest part of the difference in NO3- consumption between the elevated and ambient [CO2] treatments. The total amount of NO3(-)-N absorbed by roots or the amount of NO3(-)-N assimilated per unit area did not significantly differ between elevated and ambient [CO2] treatments. Instead, specific leaf organic nitrogen content declined, and NO3- accumulated in canopies growing under 1000 micromol mol-1 CO2. Our results indicated that 1000 micromol mol-1 CO2 diminished NO3- assimilation. If NO3- assimilation were impaired by high [CO2], then this offers an explanation for why organic nitrogen contents are often observed to decline in elevated [CO2] environments.

Smart, D. R.; Ritchie, K.; Bloom, A. J.; Bugbee, B. B.

1998-01-01

363

Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans  

PubMed Central

In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 ?g/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys3 and Cys9 or Cys10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents. PMID:22605963

Lee, Jong-Kook; Gopal, Ramamourthy; Seo, Chang Ho; Cheong, Hyeonsook; Park, Yoonkyung

2012-01-01

364

Herbicide Treatments Alter Carotenoid Profiles for 14C Tracer Production from Tomato (Solanum lycopersicum cv. VFNT cherry) Cell Cultures  

PubMed Central

Progress in learning about underlying carotenoid bioactivity mechanisms has been limited due to lack of commercially available radiolabeled lycopene (LYC), phytoene (PE), and phytofluene (PF). Tomato (Solanum lycopersicum cv. VFNT cherry) cell cultures have been treated to produce [14C]-PE and PF but with relatively low yields. To increase carotenoid production, two bleaching herbicides were administered during the culture incubation, 2-(4-chlorophenyl-thio) triethylamine and norflurazon separately or in combination to produce varying ratios of PE, PF, and LYC. Treatment with both herbicides resulted in optimal production of all three carotenoids. Subsequently, cultures were incubated in [14C] sucrose-containing media to produce labeled LYC, PE, and PF. Adding [14C] sucrose on day 1 of the 14-d culture incubation cycle to norflurazon-treated cultures led to a small increase in labeling efficiency compared to adding it on day 7. Improved culture conditions efficiently provided sufficient 14C-carotenoids for future cell culture and animal metabolic tracking studies. PMID:19489618

Engelmann, Nancy J.; Rogers, Randy B.; Lila, Mary Ann; Erdman, John W.

2009-01-01

365

Heavy metal accumulation and tolerance of energy grass (Elymus elongatus subsp. ponticus cv. Szarvasi-1) grown in hydroponic culture.  

PubMed

Phytoremediation is a plant based, cost effective technology to detoxify or stabilise contaminated soils. Fast growing, high biomass, perennial plants may be used not only in phytoremediation but also in energy production. Szarvasi-1 energy grass (Elymus elongatus subsp. ponticus cv. Szarvasi-1), a good candidate for this combined application, was grown in nutrient solution in order to assess its Cd, Cu, Ni, Pb and Zn accumulation and tolerance. Its shoot metal accumulation showed the order Pb < Ni < Cu ? Cd < Zn. In parallel with this, Pb and Ni had no or very little influence on the growth, dry matter content, chlorophyll concentration and transpiration of the plants. Cu and Cd treatment resulted in significant decreases in all these parameters that can be attributed to Fe plaque formation in the roots suggested by markedly increased Fe and Cu accumulation. This came together with decreased shoot and root Mn concentrations in both treatments while shoot Cu and Zn concentrations decreased under Cd and Cu exposure, respectively. Zn treatment had no effect or even slightly stimulated the plants. This may be due to a slight stimulation of Fe translocation and a very efficient detoxification mechanism. Based on the average 300 mg kg?¹ (dry mass) Zn concentration which is 0.03% of the shoot dry mass the variety is suggested to be classified as Zn accumulator. PMID:23669138

Sipos, Gyula; Solti, Adám; Czech, Viktória; Vashegyi, Ildikó; Tóth, Brigitta; Cseh, Edit; Fodor, Ferenc

2013-07-01

366

The CC-V Flap: A Novel Technique for Augmenting a C-V Nipple Reconstruction Using a Free Dermal Graft  

PubMed Central

BACKGROUND We present a novel method for augmenting the standard C-V flap used for nipple reconstruction with a free dermal graft which aims to improve the appearance of the nipple reconstruction, decrease loss of projection and improve patient satisfaction overall. METHODS The surgical technique for performing a free dermal graft augmentation of a CV flap is described. All patients who underwent this technique between February 2009 and January 2012 at our unit were contacted by telephone, questioned about any complications and asked to rate their satisfaction with the technique. RESULTS In a series of 18 nipple reconstructions, there were no immediate post-operative complications and long term follow up shows that that this technique achieves high patient satisfaction scores. CONCLUSIONS The CC-V flap is a safe technique which scores highly with patients for cosmetic appearance after long term follow up. PMID:25489517

Elizabeth Clark, Sarah; Turton, EPL

2014-01-01

367

The use of Lactobacillus pentosus 1MO to shorten the debittering process time of black table olives (Cv. Itrana and Leccino): a pilot-scale application.  

PubMed

Fifty lactobacilli isolated from black table olive brines were evaluated for their salt tolerance, resistance to oleuropein and verbascoside, and ability to grow in modified filter-sterilized brines. A strain of Lactobacillus pentosus was selected and used as a starter to ferment, in pilot plant, black olives (Itrana and Leccino cv.) in brines modified for pH, carbohydrate, and growth factor concentrations, at 28 degrees C. The temperature-controlled fermentation of Leccino cv. olives resulted in obtaining ready-to-eat, high-quality table olives in a reduced-time process. HPLC analysis of phenolic compounds from fermented olives showed a decrease of oleuropein, a glucoside secoiridoid responsible for the bitter taste of olive drupes, and an increase of the hydroxytyrosol concentration. The selected strain of L. pentosus (1MO) allowed the reduction of the debittering phase period to 8 days. PMID:16719508

Servili, Maurizio; Settanni, Luca; Veneziani, Gianluca; Esposto, Sonia; Massitti, Ombretta; Taticchi, Agnese; Urbani, Stefania; Montedoro, Gian Francesco; Corsetti, Aldo

2006-05-31

368

Petrography, stable isotope compositions, microRaman spectroscopy, and presolar components of Roberts Massif 04133: A reduced CV3 carbonaceous chondrite  

NASA Astrophysics Data System (ADS)

Here, we report the mineralogy, petrography, C-N-O-stable isotope compositions, degree of disorder of organic matter, and abundances of presolar components of the chondrite Roberts Massif (RBT) 04133 using a coordinated, multitechnique approach. The results of this study are inconsistent with its initial classification as a Renazzo-like carbonaceous chondrite, and strongly support RBT 04133 being a brecciated, reduced petrologic type >3.3 Vigarano-like carbonaceous (CV) chondrite. RBT 04133 shows no evidence for aqueous alteration. However, it is mildly thermally altered (up to approximately 440 °C); which is apparent in its whole-rock C and N isotopic compositions, the degree of disorder of C in insoluble organic matter, low presolar grain abundances, minor element compositions of Fe,Ni metal, chromite compositions and morphologies, and the presence of unequilibrated silicates. Sulfides within type I chondrules from RBT 04133 appear to be pre-accretionary (i.e., did not form via aqueous alteration), providing further evidence that some sulfide minerals formed prior to accretion of the CV chondrite parent body. The thin section studied contains two reduced CV3 lithologies, one of which appears to be more thermally metamorphosed, indicating that RBT 04133, like several other CV chondrites, is a breccia and thus experienced impact processing. Linear foliation of chondrules was not observed implying that RBT 04133 did not experience high velocity impacts that could lead to extensive thermal metamorphism. Presolar silicates are still present in RBT 04133, although presolar SiC grain abundances are very low, indicating that the progressive destruction or modification of presolar SiC grains begins before presolar silicate grains are completely unidentifiable.

Davidson, Jemma; Schrader, Devin L.; Alexander, Conel M. O'd.; Lauretta, Dante S.; Busemann, Henner; Franchi, Ian A.; Greenwood, Richard C.; Connolly, Harold C.; Domanik, Kenneth J.; Verchovsky, Alexander

2014-12-01

369

Managing rhodes grass (Chloris gayana) cv. Callide to improve diet quality. 1. Effects of age of regrowth, strip grazing and mulching  

Microsoft Academic Search

Two experiments assessed grazing management strategies for irrigated and nitrogen- fertilised rhodes grass (Chloris gayana) cv. Callide pas- ture. In Experiment 1, the 4 treatments were: (i) open grazing of the entire paddock on a 2-week cycle (Open); (ii) grazing on a 2-week, daily strip-grazing rotation (2); (iii) a 4-week, daily strip-grazing rotation (4); and (iv) a 6-week, daily strip-grazing

W. K. EHRLICH; R. T. COWAN; K. F. LOWE

370

Analysis of the Expression of Anthocyanin Pathway Genes in Developing Vitis vinifera 1. cv Shiraz Grape Berries and the - Implications for Pathway Regdation  

Microsoft Academic Search

Anthocyanin synthesis in Vitis vinifera L. cv Shiraz grape berries began 1 O weeks postflowering and continued throughout berry ripening. Expression of seven genes of the anthocyanin biosynthetic pathway (phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone-3-hydroxylase (F3Hl, dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygen- ase (LDOX), and UDP glucose-flavonoid 3-o-glucosyl transferase (UFCT)) was determined. In flowers and grape

Paul K. BOSS; Christopher Davies; Simon P. Robinson

371

Long-Term Fungal Inhibitory Activity of Water-Soluble Extracts of Phaseolus vulgaris cv. Pinto and Sourdough Lactic Acid Bacteria during Bread Storage  

Microsoft Academic Search

The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts

Rossana Coda; Carlo G. Rizzello; Franco Nigro; Maria De Angelis; Philip Arnault; Marco Gobbetti

2008-01-01

372

Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester induces systemic resistance in tomato ( Lycopersicon esculentum. Mill cv. Vollendung) to Cucumber mosaic virus  

Microsoft Academic Search

The potential of benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) to trigger systemic acquired resistance (SAR) in tomato (Lycopersicon esculentum. Mill cv. Vollendung) plants against a yellow strain of Cucumber mosaic virus (CMV-Y) was investigated. Application of BTH, as a drench, 7 days before inoculation with CMV-Y, protected plants against the necrosis caused by CMV-Y. The resistance was evident as a decreased

Ghandi H. Anfoka

2000-01-01

373

Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR)  

Microsoft Academic Search

In Agrobacterium-mediated genetic transformation of cotton ( Gossypium hirsutum L. cv. Coker 310FR) the frequency at which somatic embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. We used Agrobacterium strain GV3101 containing the binary vector pGSFR with the nos-nptII gene for in vitro selection and the 35S  gus-int fragment as a reporter

B. Chaudhary; S. Kumar; K. V. S. K. Prasad; G. S. Oinam; P. K. Burma; D. Pental

2003-01-01

374

Aflatoxin — induced alteration in the levels of membrane chemicals of subcellular organelles isolated from excised, incubated Glycine max, cv. ‘Essex’ roots  

Microsoft Academic Search

Isolates of aflatoxin-producing strains of Aspergillus grow on autoclaved and field-grown (lesser extent) Glycine max beans. Both mixed and aflatoxin B1 inhibit G. max, cv. ‘Essex’ bean germination and elongation of either attached or excised cultured roots. Because B1 impairs the latter roots' ability to intracellularize [14C]-leucine, it may alter plasmalemma structure and\\/or function. To determine whether incubation of excised

J. M. Danley; S. Staggers; S. Walker; A. Varnert; G. C. Llewellyn; W. V. Dashek

1981-01-01

375

Mineralogy and petrography of amoeboid olivine aggregates from the reduced CV3 chondrites Efremovka, Leoville and Vigarano: Products of nebular condensation, accretion and annealing  

NASA Astrophysics Data System (ADS)

Amoeboid olivine aggregates (AOAs) from the reduced CV chondrites Efremovka, Leoville and Vigarano are irregularly-shaped objects, up to 5 mm in size, composed of forsteritic olivine (Fa<10) and a refractory, Ca,Al-rich component. The AOAs are depleted in moderately volatile elements (Mn, Cr, Na, K), Fe,Ni-metal and sulfides and contain no low-Ca pyroxene. The refractory component consists of fine-grained Ca,Al-rich inclusions (CAIs) composed of Al-diopside, anorthite (An100), and magnesium-rich spinel (~1 wt% FeO) or fine-grained intergrowths of these minerals; secondary nepheline and sodalite are very minor. This indicates that AOAs from the reduced CV chondrites are more pristine than those from the oxidized CV chondrites Allende and Mokoia. Although AOAs from the reduced CV chondrites show evidence for high temperature nebular annealing (e.g., forsterite grain boundaries form 120 deg triple junctions) and possibly a minor degree of melting of Al-diopside-anorthite materials, none of the AOAs studied appear to have experienced extensive (>50%) melting. We infer that AOAs are aggregates of high temperature nebular condensates, which formed in CAI-forming regions, and that they were absent from chondrule-forming regions at the time of chondrule formation. The absence of low-Ca pyroxene and depletion in moderately volatile elements (Mn, Cr, Na, K) suggest that AOAs were either removed from CAI-forming regions prior to condensation of these elements and low-Ca pyroxene or gas-solid condensation of low-Ca-pyroxene was kinetically inhibited.

Komatsu, Mutsumi; Krot, Alexander N.; Petaev, Mikhail I.; Ulyanov, Alexander A.; Keil, Klaus; Miyamoto, Masamichi

2001-05-01

376

Differential gene expression analysis by cDNA-AFLP between flower buds of Phalaenopsis Hsiang Fei cv. H. F. and its somaclonal variant  

Microsoft Academic Search

Somaclonal variation occurs during plant tissue culture and introduces changes that can result in the development of desirable traits. Using cDNA-amplified restriction fragment length polymorphism (cDNA-AFLP) analysis, we compared gene expression patterns between flower buds from wild type (donor) plants of Phalaenopsis Hsiang Fei cv. H. F., whose flowers are bronze in color, and from its somaclonal variants, whose flowers

Tsai-Wei Hsu; Wen-Chieh Tsai; Dan-Ping Wang; Sandy Lin; Yu-Yun Hsiao; Wen-Huei Chen; Hong-Hwa Chen

2008-01-01

377

Does nitrogen supply affect the response of wheat (Triticum aestivum cv. Hanno) to the combination of elevated CO2 and O3?  

Microsoft Academic Search

Spring wheat (Triticum aestivum cv. Hanno) was grown at ambient (350 mmol mol? 1) or elevated CO2 (700 mmol mol? 1) in charcoaluPurafil1-filtered air (CFA - 5n mol mol ? 1) or ozone (CFA q75 nmol mol? 1 7h d? 1) at three levels of N supply (1.5, 4 and 14 mM NO?3 ), to test the hypothesis that the

Joao Cardoso-Vilhena; Jeremy Barnes

2001-01-01

378

Lack of effect of aspirin in primary CV prevention in type 2 diabetic patients with nephropathy: results from 8 years follow-up of NID-2 study.  

PubMed

The risk-to-benefit ratio for the use of low dose of aspirin in primary cardiovascular (CV) prevention in patients with diabetes mellitus remains to be clarified. We assessed the effect of aspirin on risk of CV events in type 2 diabetic patients with nephropathy, in order to verify the usefulness of Guidelines in clinical practice. We carried out a prospective multicentric study in 564 patients with type 2 diabetic nephropathy free of CV disease attending outpatient diabetes clinics . A total of 242 patients received antiplatelet treatment with aspirin 100 mg/day (group A), and 322 were not treated with antiplatelet drugs (group B). Primary end point was the occurrence of total major adverse cardio-vascular events (MACE). Secondary end points were the relative occurrence of fatal MACE. The average follow-up was 8 years. Total MACE occurred in 49 patients from group A and in 52 patients from group B. Fatal MACE occurred in 22 patients from group A and in 20 from group B; nonfatal MACE occurred in 27 patients from group A and in 32 patients from group B. Kaplan-Meier analysis did not show a statistically significant difference of cumulative MACE between the two groups. A not statistically significant difference in the incidence of both fatal (p = 0.225) and nonfatal CV events (p = 0.573) between the two groups was observed. These results were confirmed after adjustment for confounders (HR for MACE 1.11, 95 % CI 0.91-1.35). These findings suggest that low dose of aspirin is ineffective in primary prevention for patients with nephropathy. PMID:25109286

Sasso, Ferdinando Carlo; Marfella, Raffaele; Pagano, Antonio; Porta, Giovanni; Signoriello, Giuseppe; Lascar, Nadia; Minutolo, Roberto; Carbonara, Ornella; Persico, Marcello; Piscione, Federico; De Nicola, Luca; Torella, Roberto; Paolisso, Giuseppe

2015-04-01

379

Application of silver nanoparticles contained in ethanol as a working fluid in an oscillating heat pipe with a check valve (CLOHP/CV): a thermodynamic behaviour study  

NASA Astrophysics Data System (ADS)

This research focused on the thermal behaviour of oscillating heat pipe with a check valve (CLOHP/CV). The CLOHP/CV was made from a copper capillary tube with an internal diameter of 2.03 mm, 40 turns, and 2 check valves. The CLOHP/CV had three sections: the evaporator and adiabatic condenser of a length of 50, 100, and 150 mm. The angles of inclinations were 90°, 80°, 60°, 40°, 20° and 0° at normal operating conditions. The two working fluids were an ethanol and silver nanoparticles concentration of 0.5 wt% contained in the ethanol (NE). The filling ratio was 50 % with respect to the total volume of the tube. The operating temperature (T w ) was 45, 65, 85, 105, 125 and 150 °C. The results of the study showed that the heat flux increased significantly when the operating temperature increased, and the heat flux increased when the L e decreased from 150 to 50 mm. Furthermore, the thermal behaviour of CLOHP/CV showed the highest heat flux of 2,012.63 W/m2 at L e of 50, with an angle of inclination of 90° and operating temperature of 150 °C with NE. The behaviour of 0.5 wt% of silver nanoparticles contained in the ethanol produced a good contact angle. The 0.5 wt% of silver nanoparticles decreased the wet ability, thus increasing the thermal behaviour. The optimum concentration for the addition of silver nanoparticles in the working fluid was 0.5 wt%.

Bhuwakietkumjohn, N.; Parametthanuwat, T.

2015-01-01

380

Aging skeletal muscle shows a drastic increase in the small heat shock proteins ?B-crystallin\\/HspB5 and cvHsp\\/HspB7  

Microsoft Academic Search

Most heat shock proteins operate as molecular chaperones and play a central role in the maintenance of normal cellular function. In skeletal muscle, members of the ?-crystallin domain-containing family of small heat shock proteins are believed to form a cohort of essential stress proteins. Since ?B-crystallin (?BC\\/HspB5) and the cardiovascular heat shock protein (cvHsp\\/HspB7) are both implicated in the molecular

Philip Doran; Joan Gannon; Kathleen O’Connell; Kay Ohlendieck

2007-01-01

381

Zygomatic Smile Detection: The Semi-Supervised Haar Training of a Fast and Frugal System: A Gift to OpenCV Community  

Microsoft Academic Search

Five different OpenCV-compatible XML haar cascades of zygomatic smile detectors as well as five SMILEsamples from which these detectors were derived had been trained and are presented hereby as a new open source SMILEsmileD package. Samples have been extended in an incremental learning fashion, exploiting previously trained detector in order to add and label new elements of positive example set.

D. D. Hromada; C. Tijus; S. Poitrenaud; J. Nadel

2010-01-01

382

Isolation of disease-tolerant cotton ( Gossypium hirsutum L. cv. SVPR 2) plants by screening somatic embryos with fungal culture filtrate  

Microsoft Academic Search

We report an in vitro selection method that has led to isolation of Fusarium wilt and Alternaria leaf spot disease-tolerant plantlets in cotton (Gossypium hirsutum L. cv. SVPR2). Embryogenic callus was isolated from hypocotyl explants of cotton cultured on 5–50% Fusarium oxysporum culture filtrate-fortified callus induction medium. Somatic embryos tolerant to fungal culture filtrate (FCF) were isolated from this embryogenic

M. Ganesan; N. Jayabalan

2006-01-01

383

ICP OES and CV AAS in determination of mercury in an unusual fatal case of long-term exposure to elemental mercury in a teenager.  

PubMed

In this work, a case of deliberate self-poisoning is presented. A 14-year-old girl suddenly died during one of the several hospitalizations. Abdominal computer tomography showed a large number of metallic particles in the large intestine. Analysis of blood and internal organs for mercury and other toxic metals carried out by inductively coupled plasma optical emission spectrometry (ICP OES) revealed high concentrations of mercury in kidneys and liver (64,200 and 2470ng/g, respectively), less in stomach (90ng/g), and none in blood. Using cold vapor-atomic absorption spectrometry (CV AAS), high levels of mercury were confirmed in all examined materials, including blood (87ng/g), and additionally in hair. The results of analysis obtained by two techniques revealed that the exposure to mercury was considerable (some time later, it was stated that the mercury originated from thermometers that had been broken over the course of about 1 year, because of Münchausen syndrome). CV AAS is a more sensitive technique, particularly for blood samples (negative results using ICP OES), and tissue samples - with LOQ: 0.63ng/g of Hg (CV AAS) vis-à-vis 70ng/g of Hg (ICP OES). However, ICP OES may be used as a screening technique for autopsy material in acute poisoning by a heavy metal, even one as volatile as mercury. PMID:24630410

Lech, Teresa

2014-04-01

384

Infection of CV1 cells expressing the polyoma virus middle T antigen or the SV40 agnogene product with simian virus 40 host-range mutants.  

PubMed

SV40 viruses bearing mutations at the carboxy-terminus of large T antigen exhibit a host-range phenotype: such viruses are able to grow in BSC monkey kidney cells at 37 degrees C, but give at least 10,000-fold lower yields than wild type virus in BSC cells at 32 degrees C or in CV1 monkey kidney cells at either temperature. The block to infection in the nonpermissive cell type occurs after the onset of viral DNA replication. Infectious progeny virions are produced at very low efficiency. Although capsid proteins are synthesized at decreased levels, this does not account for the magnitude of the defect. Presumably some step of virion assembly or maturation is affected in these mutants. We have previously reported that the viral agnogene product, a protein thought to be involved in viral assembly or release, fails to accumulate in CV1 cells infected with host-range mutants. In polyoma virus the middle T antigen plays a role in virion maturation by influencing the phosphorylation of capsid proteins. In this communication we show that host-range mutants fail to undergo productive infection of CV1 cells expressing middle T antigen. These mutants do form plaques on an agnoprotein-expressing cell line. However, the agnoprotein does not seem to act by correcting the mutational block but rather increases the efficiency of plaque formation. PMID:2162817

Spence, S L; Tack, L C; Wright, J H; Carswell, S; Pipas, J M

1990-06-01

385

Hydrothermal origin of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A CAI from the Northwest Africa 2086 CV3 chondrite  

NASA Astrophysics Data System (ADS)

We report an occurrence of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A calcium-aluminum-rich inclusion (CAI) from the CV3 (Vigarano-like) carbonaceous chondrite Northwest Africa 2086. Dmisteinbergite occurs as approximately 10 ?m long and few micrometer-thick lath-shaped crystal aggregates in altered parts of the CAI, and is associated with secondary nepheline, sodalite, Ti-poor Al-diopside, grossular, and Fe-rich spinel. Spinel is the only primary CAI mineral that retained its original O-isotope composition (?17O ~ -24‰); ?17O values of melilite, perovskite, and Al,Ti-diopside range from -3 to -11‰, suggesting postcrystallization isotope exchange. Dmisteinbergite, anorthite, Ti-poor Al-diopside, and ferroan olivine have 16O-poor compositions (?17O ~ -3‰). We infer that dmisteinbergite, together with the other secondary minerals, formed by replacement of melilite as a result of fluid-assisted thermal metamorphism experienced by the CV chondrite parent asteroid. Based on the textural appearance of dmisteinbergite in NWA 2086 and petrographic observations of altered CAIs from the Allende meteorite, we suggest that dmisteinbergite is a common secondary mineral in CAIs from the oxidized Allende-like CV3 chondrites that has been previously misidentified as a secondary anorthite.

Fintor, Krisztian; Park, Changkun; Nagy, Szabolcs; Pál-Molnár, Elemér; Krot, Alexander N.

2014-05-01

386

Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening  

PubMed Central

Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. “Kent” was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ? 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like “cell wall,” “carbohydrate catabolic process” and “starch and sucrose metabolic process” among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening. PMID:25741352

Dautt-Castro, Mitzuko; Ochoa-Leyva, Adrian; Contreras-Vergara, Carmen A.; Pacheco-Sanchez, Magda A.; Casas-Flores, Sergio; Sanchez-Flores, Alejandro; Kuhn, David N.; Islas-Osuna, Maria A.

2015-01-01

387

Effects of salicylic acid and putrescine on storability, quality attributes and antioxidant activity of plum cv. 'Santa Rosa'.  

PubMed

Plum fruit has a short shelf life with a rapid deterioration in quality after harvest. The primary goal of this study is to investigate and compare the effect of putrescine and salicylic acid on quality properties and antioxidant activity of plum during storage. The plum fruits (cv. 'Santa Rosa') were harvested at the mature ripe stage, and dipped in different concentrations of putrescine (1, 2, 3 and 4 mmol/L) and salicylic acid (1, 2, 3 and 4 mmol/L), as well as distilled water (control) for 5 min. The fruits were then packed in boxes with polyethylene covers and stored at 4 °C with 95 % relative humidity for 25 days. A factorial trial based on completely randomized block design with 4 replications was carried out. The weight loss, fruit firmness, total soluble solids, titratable acidity, pH, maturity index, ascorbic acid, total phenolics and antioxidant activity at 0, 5, 10, 15, 20 and 25 days after harvest were recorded. During the storage period, the weight loss, total soluble solids, pH and maturity index increased significantly while the fruit firmness, titratable acidity, ascorbic acid, total phenolics and antioxidant activity decreased significantly (P?

Davarynejad, Gholam Hossein; Zarei, Mehdi; Nasrabadi, Mohamad Ebrahim; Ardakani, Elham

2015-04-01

388

Cloning, expression and immunolocalization pattern of a cinnamyl alcohol dehydrogenase gene from strawberry (Fragaria x ananassa cv. Chandler).  

PubMed

Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) catalyses the conversion of p-hydroxy-cinnamaldehydes to the corresponding alcohols and is considered a key enzyme in lignin biosynthesis. By a differential screening of a strawberry (Fragariax ananassa cv. Chandler) fruit specific subtractive cDNA library, a full-length clone corresponding to a cad gene was isolated (Fxacad1). Northern blot and quantitative real time PCR studies indicated that the strawberry Fxacad1 gene is expressed in fruits, runners, leaves, and flowers but not in roots. In addition, the gene presented a differential expression in fruits along the ripening process. Moreover, by screening of a strawberry genomic library a cad gene was isolated (Fxacad2). Similar to that found in other cad genes from higher plants, this strawberry cad gene is structured in five exons and four introns. Southern blot analyses suggest that, probably, a small cad gene family exists in strawberry. RT-PCR studies indicated that only the Fxacad1 gene was expressed in all the fruit ripening stages and vegetative tissues analysed. The Fxacad1 cDNA was expressed in E. coli cells and the corresponding protein was used to raise antibodies against the strawberry CAD polypeptide. The antibodies obtained were used for immunolocalization studies. The results showed that the CAD polypeptide was localized in lignifying cells of all the tissues examined (achenes, fruit receptacles, runners, leaves, pedicels, and flowers). Additionally, the cDNA was also expressed in yeast (Pichia pastoris) as an extracellular protein. The recombinant protein showed activity with the characteristic substrates of CAD enzymes from angiosperms, indicating that the gene cloned corresponds to a CAD protein. PMID:12147722

Blanco-Portales, R; Medina-Escobar, N; López-Ráez, J A; González-Reyes, J A; Villalba, J M; Moyano, E; Caballero, J L; Muñoz-Blanco, J

2002-08-01

389

A lectin with highly potent inhibitory activity toward breast cancer cells from edible tubers of Dioscorea opposita cv. nagaimo.  

PubMed

A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC(50) values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

Chan, Yau Sang; Ng, Tzi Bun

2013-01-01

390

FANTINA: Fathom Asteroids Now: Tomography and Imagery of a NEA- Payload For Marco Polo R CV3 / ESA mission  

NASA Astrophysics Data System (ADS)

The internal structure of small bodies is still poorly known and has never been measured directly. There is no way to determine from ground based observation whether the body is a monolithic piece of rock or a rubble-pile, an aggregate of boulders held together by gravity and how much porosity it contains, both in the form of micro-scale or macro-scale porosity. Knowing this structure is a key point for a better understanding of the asteroid accretion and dynamical evolution. It is the main objective of the FANTINA experience proposed Payload for Marco Polo R CV3 / ESA mission: FANTINA (Marco Polo's Daughter) for Fathom Asteroids Now: Tomography and Imagery of a NEA is to provide measurement capabilities and science data which are not accessible by remote sensing of the asteroid from the Marco-Polo R spacecraft alone and which complement the analysis of the returned samples. The FANTINA payload is a Lander carrying the ASSERT experiment and a complementary payload to be defined: ASSERT is a bistatic CONSERT/Rosetta-like radar to achieve the tomography both in transmission and in reflexion of the asteroid in order to characterise its composition and its heterogeneity from decimetric to global scale. The lander is a long-lived bus in the 10-kg class derived from MASCOT/Hayabusa 2. This paper reviews the science rationale of FANTINA in the context of the Marco Polo R mission to 1996FG3. The surface package concept will be reviewed including the radar tomography principles. So a preliminary design and budget will be done.

Herique, A.; Biele, J.; Bousquet, P.; Ciarletti, V.; Ho, T. M.; Issler, J. L.; Kofman, W.; Michel, P.; Plettemeier, D.; Puget, P.; Souyris, J. C.; Ulamec, S.; van Zoest, T.; Zine, S.

2012-04-01

391

Olivine-rich rims surrounding chondrules in the Mokoia CV3 carbonaceous chondrite: Further evidence for parent-body processes  

NASA Astrophysics Data System (ADS)

Fine-grained rims surrounding chondrules and inclusions in the Mokoia CV3 carbonaceous chondrite can be divided into phyllosilicate-rich and olivine-rich types. We present a petrographic and electron microscopic study of the olivine-rich rims and their host objects (referred to as chondrules/olivine-rich rims). The olivine-rich rims consist mainly of Fe-rich olivine and very minor phyllosilicate (saponite). Their host chondrules contain minor saponite and phlogopite, which resulted from aqueous alteration of anhydrous silicates. Mineralogical and compositional characteristics of the chondrules/olivine-rich rims suggest that they experienced mild thermal metamorphic effects. The rims commonly contain veins of coarse-grained Fe-rich olivine, magnetite, and Fe-(Ni) sulfides. The chondrules show abundant evidence of alteration along their peripheries, and the alteration textures suggest a mechanism for rim formation by replacement of the chondrules. Initially, enstatite and opaque nodules preferentially reacted to form coarse, platy, Fe-rich olivine crystals, which were subsequently divided into finer grains. Forsterite was also replaced by Fe-rich olivine. As the alteration advanced, these Fe-rich olivines were disaggregated, mixed with simultaneously produced saponite, and formed rims. In contrast, the surrounding matrix shows no evidence of such alteration and metamorphism. These observations indicate that the chondrules/olivine-rich rims did not experience these secondary processes in their present setting. The results suggest that the chondrules/olivine-rich rims experienced extensive replacement reactions in an environment in which aqueous fluids existed but only in minor amounts. They have probably also undergone simultaneous and/or subsequent mild thermal metamorphism. We suggest that the chondrules/olivine-rich rims are actually clasts transported from a relatively dry region in the parent body that was different from the region where Mokoia was finally lithified.

Tomeoka, Kazushige; Ohnishi, Ichiro

2014-07-01

392

Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.  

PubMed

Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ? 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic process" among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening. PMID:25741352

Dautt-Castro, Mitzuko; Ochoa-Leyva, Adrian; Contreras-Vergara, Carmen A; Pacheco-Sanchez, Magda A; Casas-Flores, Sergio; Sanchez-Flores, Alejandro; Kuhn, David N; Islas-Osuna, Maria A

2015-01-01

393

Photosynthesis and growth responses of mustard (Brassica juncea L. cv Pusa Bold) plants to free air carbon dioxide enrichment (FACE).  

PubMed

Increased atmospheric [CO2] is likely to affect photosynthesis, plant growth, and yield potential of plants. Mustard (Brassica juncea L.) is an important oil seed crop that is widely grown in India. Therefore, the impact of elevated [CO2] (585 ?mol mol(-1)) on pigment and protein content, chlorophyll a fluorescence, photosynthetic electron transport reactions, CO2 assimilation, biomass production, and seed yield potential was measured in B. juncea cv Pusa Bold, grown inside free air carbon dioxide enrichment (FACE) rings installed on the campus of Jawaharlal Nehru University, New Delhi, India. Plants were grown for three consecutive winter seasons (2010-2013), in ambient (385 ?mol mol(-1)) or elevated [CO2], in field conditions. Elevated [CO2] had no significant effect on the minimal chlorophyll fluorescence (F 0), while the quantum efficiency of Photosystem II, measured as variable fluorescence (F v?=?F m-F 0) to maximum fluoresence (F m), increased by 3 %. Electron transport rate, photosystem I, photosystem II, and whole chain electron transport rates increased by 8 % in elevated [CO2]. However, the net photosynthesis rate increased by ?50 % in three growing seasons under elevated [CO2] condition. The stomatal conductance and transpiration rate decreased resulting in higher photosynthetic water use efficiency. The photosynthesizing surface, i.e., leaf area index substantially increased leading to higher biomass and seed yield under elevated [CO2] condition. Acclimatory downregulation of photosynthesis and plant productivity was not observed in three consecutive growing years suggesting that in the absence of nutrient limitation, B. juncea is highly responsive to elevated CO2 whose yield potential shall increase in changing climatic conditions. PMID:25471475

Ruhil, Kamal; Sheeba; Ahmad, Altaf; Iqbal, Muhammad; Tripathy, Baishnab C

2014-12-01

394

The effects of tropospheric ozone and elevated carbon dioxide on potato (Solanum tuberosum L. cv. Bintje) growth and yield.  

PubMed

An open-top chamber experiment with field grown potato (Solanum tuberosum L. cv. Bintje) was performed in the south-west of Sweden in 1998. The experiment was aimed to investigate individual and interactive impacts of elevated ozone (O(3)) and carbon dioxide (CO(2)) on potato growth and yield. Treatments were ambient and elevated CO(2) with or without the addition of 20 nmol mol(-1) O(3). In addition, plants were grown in ambient air plots. Plants grown in elevated CO(2) and O(3) had a reduced tuber yield, compared to control, by 2% and 8%, respectively. These effects were, however, not statistically significant. Elevated CO(2) caused a significantly larger number of tubers of lower average size, while elevated O(3) induced significantly lower tuber dry matter content. The number of tuber initials (tubers <15 mm) found at harvest was significantly lower in elevated O(3). Visible O(3) leaf injury appeared earlier and the number of yellow leaves 89 days after emergence was significantly larger in elevated O(3). In elevated CO(2), haulm dry weight was significantly (15%) lower at harvest and the haulm/tuber ratio was significantly reduced. There were significant interactive effects of O(3) and CO(2) on the final number of leaves and on stem height. Plants in elevated CO(2) reached final stem height earlier than in the other treatments. In summary, it was concluded: (a) that anticipated future O(3) levels are likely to have a negative impact on potato growth, although tuber yield effects may be hard to demonstrate experimentally in this plant due to large variation; (b) that whether any possible beneficial effect of elevated CO(2) on the magnitude of tuber yield will be expressed, depend on the climatic conditions prevailing; and (c) that potato growth responses to CO(2) and O(3) are not simply additive. PMID:12812743

Persson, K; Danielsson, H; Selldén, G; Pleijel, H

2003-07-01

395

A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata  

PubMed Central

Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays comprising 14?500 probes were used to compare, under three experimental conditions (in vitro, in the greenhouse, and in the vineyard), foliar material of infected symptomatic plants (S+R+), infected asymptomatic plants (S–R+), and healthy plants (S–R–). These plants were characterized by symptom notation after natural (vineyard) or experimental (in vitro and greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative real-time PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (Erysiphe necator, Plasmopara viticola, and Botrytis cinerea). (i) Five functional categories of genes, that is those involved in metabolism, defence reactions, interaction with the environment, transport, and transcription, were up-regulated in S+R+ plants compared with S–R– plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also up-regulated after infection by powdery mildew, downy mildew, and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and the polypeptide fraction secreted by Eutypa. PMID:20190040

Camps, Céline; Kappel, Christian; Lecomte, Pascal; Léon, Céline; Gomès, Eric; Coutos-Thévenot, Pierre; Delrot, Serge

2010-01-01

396

A Lectin with Highly Potent Inhibitory Activity toward Breast Cancer Cells from Edible Tubers of Dioscorea opposita cv. Nagaimo  

PubMed Central

A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC50 values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

Chan, Yau Sang; Ng, Tzi Bun

2013-01-01

397

Control of endogenous bacterial contamination and micropropagation of a traditional table banana (Musa spp. cv. Kanthali) of Bangladesh.  

PubMed

Shoot tips of a traditional table banana (Musa spp. cv. Kanthali) of Bangladesh were evaluated for in vitro propagation. Initial surface sterilization (with 0.1% HgCl2 for 12 minutes) of shoot tips was successful but microbial contamination (mostly bacteria) at the rhizomatous base of the explants was observed within 6-15 days after inoculation which eventually killed 85% of inoculated explants. So, for contamination free culture establishment explants were soaked in two broad spectrum antibiotics namely ampicillin and gentamicin. Cent percent contamination free cultures were established by soaking the explants in 400 mg/L ampicillin or 200 mg/L gentamicin for 1h. Antibiotic treated explants were found to be full contamination free but failed to regenerate after 3 weeks of culture. But some of them absorbed media for up to 2nd subculture and showed swelling of explants and some color changes from pale white to light/deep green. Finally, a few days after 3rd subculture, no growth of explants was observed and all treated explants eventually started to die. Among the untreated alive explants the best medium for single shoot development was MS + 4.0 mg/L BA + 0.5 mg/L KT + 15% CW and average time required for shoot development was 18-21 days. But the regeneration percentage was very low (30%). The best medium for shoot multiplication was MS + 4.0 mg/L BA + 2.0 mg/L IAA + 15% CW and only average 3-4 shoots were formed per shoot. Finally, in vitro proliferated shoots produced roots with maximum frequency (90%) in half strength of MS medium fortified with 0.5 mg/L IBA. PMID:18257234

Titov, Soubir; Bhowmik, Salil Kumar; Alam, Md Sadrul; Uddin, Sarder Nasir

2007-11-01

398

Structural analysis of wheat wax (Triticum aestivum, c.v. 'Naturastar' L.): from the molecular level to three dimensional crystals.  

PubMed

In order to elucidate the self assembly process of plant epicuticular waxes, and the molecular arrangement within the crystals, re-crystallisation of wax platelets was studied on biological and non-biological surfaces. Wax platelets were extracted from the leaf blades of wheat (Triticum aestivum L., c.v. 'Naturastar', Poaceae). Waxes were analysed by gas chromatography (GC) and mass spectrometry (MS). Octacosan-1-ol was found to be the most abundant chemical component of the wax mixture (66 m%) and also the determining compound for the shape of the wax platelets. The electron diffraction pattern showed that both the wax mixture and pure octacosan-1-ol are crystalline. The re-crystallisation of the natural wax mixture and the pure octacosan-1-ol were studied by scanning tunnelling microscopy (STM), atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Crystallisation of wheat waxes and pure octacosano-1-ol on the non polar highly ordered pyrolytic graphite (HOPG) led to the formation of platelet structures similar to those found on the plant surface. In contrast, irregular wax morphologies and flat lying plates were formed on glass, silicon, salt crystals (NaCl) and mica surfaces. Movement of wheat wax through isolated Convallaria majalis cuticles led to typical wax platelets of wheat, arranged in the complex patterns typical for C. majalis. STM of pure octacosan-1-ol monolayers on HOPG showed that the arrangement of the molecules strictly followed the hexagonal structure of the substrate crystal. Re-crystallisation of wheat waxes on non-polar crystalline HOPG substrate showed that technical surfaces could be used to generate microstructured, biomimetic surfaces. AFM and SEM studies proved that a template effect of the substrate determined the orientation of the re-grown crystals. These effects of the structure and polarity of the substrate on the morphology of the epicuticular waxes are relevant for understanding interactions between biological as well as technical surfaces and waxes. PMID:16133211

Koch, K; Barthlott, W; Koch, S; Hommes, A; Wandelt, K; Mamdouh, W; De-Feyter, S; Broekmann, P

2006-01-01

399

Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)  

SciTech Connect

Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the plasma membrane, accounting for 16 mole % of the total lipid. Phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine with lesser amounts of phosphatidylglycerol, phosphatidic acid, phosphatidylserine, and phosphatidylinositol, comprised only 32 mole% of the total lipid in NA samples. Following cold acclimation, free sterols increased from 33 to 44 mole %, while steryl glucosides and acylated steryl glucosides decreased from 15 to 6 mole % and 4 to 1 mole %, respectively. Sterol analyses of these lipid classes demonstrated that free {beta}-sitosterol increased from 21 to 32 mole % (accounting for the increase in free sterols as a class) at the expense of sterol derivatives containing {beta}-sitosterol. Glucocerebrosides decreased from 16 to 7 mole % of the total lipid following cold acclimation. In addition, the relative proportions of associated hydroxy fatty acids, including 22:0 (h), 24:0 (h), 22:1 (h), and 24:1 (h) were altered. The phospholipid content of the plasma membrane fraction increased to 42 mole % of the total lipid following cold acclimation. Although the relative proportions of the individual phospholipids did not change appreciably after cold acclimation, there were substantial differences in the molecular species. Di-unsaturated molecular species of phosphatidylcholine and phosphatidylethanolamine increased following acclimation. These results demonstrate that cold acclimation results in substantial changes in the lipid composition of the plasma membrane.

Lynch, D.V.; Steponkus, P.L. (Cornell Univ., Ithaca, NY (USA))

1987-01-01

400

Hudiburg CV Tara Hudiburg  

E-print Network

Forests · Advisor ­ Professor Beverly Law B.S. Biology (1998), Pacific Lutheran University, Tacoma, WA, Pacific Lutheran University Courses: Principles of Biology core sequence, Field Methods of Ecology #12;L

DeLucia, Evan H.

401

John Cushman's CV  

E-print Network

Society for Industrial and Applied Math; American Geophysical Union (Fellow); Society of Engineering Science; Soil Science Society of America (Fellow); Materials ... chaotic dynamics in fractal porous media with application to microbe motility. ..... Cushman, J.H. (1985) Multiphase Transport Based on Compact Distributions.

F. Alejandro Bonilla

402

Xu Zhang's Homepage - CV  

E-print Network

T. Lin, Y. Lin and X. Zhang, A Method of Lines Based on Immersed Finite ... Element Methods and Application to Plate Bending Problems, Appl. Math. ... 8, International Congress on Industrial and Applied Mathematics, Beijing, China. ... 3, SIAM Conference on Computational Science and Engineering, Salt Lake City, UT.

403

download here my CV  

E-print Network

Jan 18, 2012 ... Curriculum Vitae ... 2008-Now: Tenured Associate Professor of Mathematics at Purdue ... Member of the committee that is in charge of the computer networks at ... Summer 2010: I supervised an undergraduate research program .... of Lichnerowicz-Szabo theorem, Bulletin des Sciences Mathématiques,. Vol.

Author

2012-03-19

404

Growth, Pigment Synthesis, and Ultrastructural Responses of Phaseolus vulgaris L. cv. Blue Lake to Intermittent and Flashing Light 12  

PubMed Central

Growing bean plants (Phaseolus vulgaris L. cv. Blue Lake) on cycles of 1 minute light-1 minute dark or 5 minutes light-5 minutes dark, providing an integrated 12 hours light-12 hours dark per day for each set of plants, led to production after 21 days of new leaves low or lacking in chloroplast pigments. Subsequently, dry weight increase was sharply cut. Leaf area was affected by the light regimes after the second week of growth. By the fourth week, plants on the 1 minute light-1 minute dark cycle showed about one-half the leaf area of the controls. Shoot growth was favored over root growth to the greatest degree on the 1 minute light-1 minute dark regimes. Chlorophyll a/b ratios were close to 3.0 in all of the intermittent light regimes, but the total amounts of chlorophyll in milligrams per primary leaf were higher from day 9 to day 23 for the 12 hour light-12 hours dark controls than for other plants. Although they produced chlorophyll, the plants receiving 1 or 2 milliseconds per second of light continued to lose weight at the same rate as the dark controls; thus, it is assumed there was no net photosynthesis. Plants receiving flashing light allocated significantly more food reserves from the seed to roots than did dark controls. Total chlorophyll formation was significantly accelerated by 2 milliseconds per second light. With 1 millisecond per second light, it took 5 days longer to achieve the same level of chlorophyll. After the 18th day, there was a steady decline in chlorophyll, b degrading more rapidly than a. It is thought that several light-driven reactions are involved in the observed pigment synthesis, photosynthesis, food allocation, and growth of bean. Some of these reactions may be cyclic and others linear. Collectively, they must reach a harmonic point for normal metabolism and development to occur. Because time courses for each of these reactions are different, the intermittent and flashing light technique offers the possibility of individually studying some of the key light-driven reactions. Images Fig. 8 Fig. 9 PMID:16662457

Naylor, Aubrey W.; Giles, Lawrence J.

1982-01-01

405

Impact of innovative controlled atmosphere storage technologies and postharvest treatments on volatile compound production in cv. Pinova apples.  

PubMed

Organically grown apples cv. Pinova harvested at two different dates were stored at 1.3 degrees C for up to 4 months in air, up to 7 months in ULO (1.5 kPa of O(2) and 1.3 kPa of CO(2)) and in dynamic controlled atmosphere (DCA) conditions (0.4-0.6 kPa of O(2) and 0.6-0.8 kPa of CO(2)); the DCA storage method involved the use of a chlorophyll fluorescence monitoring system in order to detect low-O(2) stress in apples and to allow for the dynamic adaptation of storage atmosphere to O(2) levels that were lower than in ULO but still tolerated by fruits. A postharvest 1-MCP treatment (for 24 h at 1.3 degrees C) and a hot water treatment (for 180 s at 50 degrees C) were also tested on apples stored afterward in ULO and air, respectively. Volatile compounds isolated from the pulp of fruits were measured after 4 and 7 months, just upon removal from storage and after 11 days at 22 degrees C. Total amount of aroma compounds detected in apples stored in DCA was markedly higher (from 2- to 4-fold) than in fruits exposed to 1-MCP + ULO but, at most sampling times, significantly lower than in ULO fruits. Moderate differences in storage atmosphere composition between ULO and DCA significantly affected both total amount and profile of volatile esters. Analogous effects were observed on the alcohol precursors of the main esters. Exposure to 1-MCP inhibited biosynthesis of straight-chain esters more than that of branched-chain esters. The hot water treatment did not seem to produce marked changes in volatile composition after four months of air storage, except for a sharp accumulation of aldehydes during the shelf-life time. DCA storage technology, besides avoiding any chemical treatment, can preserve apple aroma compounds better than 1-MCP + ULO during long-term storage. PMID:19154103

Raffo, Antonio; Kelderer, Markus; Paoletti, Flavio; Zanella, Angelo

2009-02-11

406

Efficacy of Pneumococcal Nontypable Haemophilus influenzae Protein D Conjugate Vaccine (PHiD-CV) in Young Latin American Children: A Double-Blind Randomized Controlled Trial  

PubMed Central

Background The relationship between pneumococcal conjugate vaccine–induced antibody responses and protection against community-acquired pneumonia (CAP) and acute otitis media (AOM) is unclear. This study assessed the impact of the ten-valent pneumococcal nontypable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) on these end points. The primary objective was to demonstrate vaccine efficacy (VE) in a per-protocol analysis against likely bacterial CAP (B-CAP: radiologically confirmed CAP with alveolar consolidation/pleural effusion on chest X-ray, or non-alveolar infiltrates and C-reactive protein ? 40 µg/ml); other protocol-specified outcomes were also assessed. Methods and Findings This phase III double-blind randomized controlled study was conducted between 28 June 2007 and 28 July 2011 in Argentine, Panamanian, and Colombian populations with good access to health care. Approximately 24,000 infants received PHiD-CV or hepatitis control vaccine (hepatitis B for primary vaccination, hepatitis A at booster) at 2, 4, 6, and 15–18 mo of age. Interim analysis of the primary end point was planned when 535 first B-CAP episodes, occurring ?2 wk after dose 3, were identified in the per-protocol cohort. After a mean follow-up of 23 mo (PHiD-CV, n?=?10,295; control, n?=?10,201), per-protocol VE was 22.0% (95% CI: 7.7, 34.2; one-sided p?=?0.002) against B-CAP (conclusive for primary objective) and 25.7% (95% CI: 8.4%, 39.6%) against World Health Organization–defined consolidated CAP. Intent-to-treat VE was 18.2% (95% CI: 5.5%, 29.1%) against B-CAP and 23.4% (95% CI: 8.8%, 35.7%) against consolidated CAP. End-of-study per-protocol analyses were performed after a mean follow-up of 28–30 mo for CAP and invasive pneumococcal disease (IPD) (PHiD-CV, n?=?10,211; control, n?=?10,140) and AOM (n?=?3,010 and 2,979, respectively). Per-protocol VE was 16.1% (95% CI: ?1.1%, 30.4%; one-sided p?=?0.032) against clinically confirmed AOM, 67.1% (95% CI: 17.0%, 86.9%) against vaccine serotype clinically confirmed AOM, 100% (95% CI: 74.3%, 100%) against vaccine serotype IPD, and 65.0% (95% CI: 11.1%, 86.2%) against any IPD. Results were consistent between intent-to-treat and per-protocol analyses. Serious adverse events were reported for 21.5% (95% CI: 20.7%, 22.2%) and 22.6% (95% CI: 21.9%, 23.4%) of PHiD-CV and control recipients, respectively. There were 19 deaths (n?=?11,798; 0.16%) in the PHiD-CV group and 26 deaths (n?=?11,799; 0.22%) in the control group. A significant study limitation was the lower than expected number of captured AOM cases. Conclusions Efficacy was demonstrated against a broad range of pneumococcal diseases commonly encountered in young children in clinical practice. Trial registration www.ClinicalTrials.gov NCT00466947 Please see later in the article for the Editors' Summary PMID:24892763

Tregnaghi, Miguel W.; Sáez-Llorens, Xavier; López, Pio; Abate, Hector; Smith, Enrique; Pósleman, Adriana; Calvo, Arlene; Wong, Digna; Cortes-Barbosa, Carlos; Ceballos, Ana; Tregnaghi, Marcelo; Sierra, Alexandra; Rodriguez, Mirna; Troitiño, Marisol; Carabajal, Carlos; Falaschi, Andrea; Leandro, Ana; Castrejón, Maria Mercedes; Lepetic, Alejandro; Lommel, Patricia; Hausdorff, William P.; Borys, Dorota; Guiñazú, Javier Ruiz; Ortega-Barría, Eduardo; Yarzábal, Juan P.; Schuerman, Lode

2014-01-01

407

Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish).  

PubMed

Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubens (Foc) is the most serious disease that attacks banana plants. Salicylic acid (SA) can play a key role in plant-microbe interactions. Our study is the first to examine the role of SA in conferring resistance to Foc TR4 in banana (Musa acuminata L. AAA group, cv. Cavendish), which is the greatest commercial importance cultivar in Musa. We used quantitative real-time reverse polymerase chain reaction (qRT-PCR) to analyze the expression profiles of 45 genes related to SA biosynthesis and downstream signaling pathways in a susceptible banana cultivar (cv. Cavendish) and a resistant banana cultivar (cv. Nongke No. 1) inoculated with Foc TR4. The expression of genes involved in SA biosynthesis and downstream signaling pathways was suppressed in a susceptible cultivar and activated in a resistant cultivar. The SA levels in each treatment arm were measured using high-performance liquid chromatography. SA levels were decreased in the susceptible cultivar and increased in the resistant cultivar. Finally, we examined the contribution of exogenous SA to Foc TR4 resistance in susceptible banana plants. The expression of genes involved in SA biosynthesis and signal transduction pathways as well as SA levels were significantly increased. The results suggest that one reason for banana susceptibility to Foc TR4 is that expression of genes involved in SA biosynthesis and SA levels are suppressed and that the induced resistance observed in banana against Foc TR4 might be a case of salicylic acid-dependent systemic acquired resistance. PMID:25277445

Wang, Zhuo; Jia, Caihong; Li, Jingyang; Huang, Suzhen; Xu, Biyu; Jin, Zhiqiang

2015-01-01

408

The effects of the temperature on IV and CV characteristics of Al\\/P2ClAn(C{2}H{5}COOH)\\/pSi\\/Al structure  

Microsoft Academic Search

The current-voltage (I-V) and capacitance-voltage (C-V) characteristics of metal-organic compound conductive polymer-semiconductor Al\\/P2ClAn(C{2}H{5}COOH)\\/p-Si structures were measured in the temperature range 80-340 K. P2ClAn: the poly(2-chloroaniline). The P2ClAn emeraldine salt was synthesized chemically by using propionic (C{2}H{5}COOH) acid. The analysis of I-V characteristics based on the thermoionic emission (TE) mechanism has revealed a decrease of zero-bias barrier height and a increase

A. F. Özdemir; Z. Kotan; D. A. Aldemir; S. Altindal

2009-01-01

409

Correlated Study of Rb-Sr Systematics and Petrologic Properties of Chondrules from Allende (CV): Evidence for Secondary Alteration  

NASA Astrophysics Data System (ADS)

In order to clarify when and where the distributions of alkalis in chondrules were established [1-3] we have undertaken a correlated study of Rb-Sr systematics and petrologic properties of chondrules from the Allende (CV) meteorite. We report here the results of Rb-Sr isotopic analyses combined with petrologic examinations for 16 chondrules (19 specimens) from Allende. The whole-rock analyses indicate a model age of 4.5 +/- 0.05 Ga (using ALL [4]), suggesting that the Rb-Sr isotopic system has been closed in bulk Allende for 4.5 yr. For the chondrules, the earlier works [4,5] include a few barred-olivine and many unknown petrographic types (probably mostly porphyritic) chondrules. In this work, we present results for 1 radial pyroxene, 4 porphyritic (7 specimens), and 11 barred olivine chondrules, covering major petrographic types. The results are shown in an 87Rb-87Sr evolution diagram (Fig. 1). Including earlier results [4,5], no systematic differences are found for different textural types, but data points mostly deviate from the 4.5-Ga line to the right, forming a rough linear array. This means that the Rb-Sr isotopic system in chondrules have been disturbed by a late event(s). The slope of the dotted line corresponds to an age of ~4.0 Ga. The meanlng of the linear trend is not clear because of the lack of isotopic equilibrium. Ground masses of chondrules analyzed for Rb-Sr isotopes were examined by optical microscope and EPMA. Some chondrules have primary glassy ground masses enriched in the anorthite component, especially in their central parts. However, as demonstrated previously [6], most of the chondrules analyzed here for Rb-Sr isotopes were also altered. They have abundant nepheline and sodalite components that replace primary glassy ground masses. Sodium was secondarily introduced and Ca was lost from the Allende chondrules. Using abundances of dodalite and nepheline components, the degree of alteration was tentatively classified into three categories: A (least altered), B (middle), and C (most altered). In Fig. 1, the chondrules with higher 87Rb/86Sr are mostly in categories of B or C. On the other hand, the chondrules of category A have the lower 87Rb/86Sr ratio, relatively close to that of bulk Allende. The 87Rb/86Sr ratios are related to the alteration categories but are not directly related to the isotopic deviation. It is thus considered that the general trend of high and low elemental ratios of Rb/Sr had been basically established during the early nebular processes and were then modified significantly more recently. The diffusivities of alkalis in glassy materials [7] at low temperatures (~400 degrees C) suggest a possible migration of Rb as well as Na from matrix to ground masses of chondrules. The old model age for Allende "matrix" [5] is consistent with such a possibility. We therefore strongly suggest that the Allende chondrules were subjected to a low- temperature alteration reaction after consolidation of the chondrules. References: [1] Grossman J. N. and Wasson J. T. (1983) In Chondrules and Their Origins (E. A. King, ed.), 88-121, LPI. [2] Hewins R. H. (1991) GCA, 55, 935-942. [3] Matsuda H. et al. (1990) Meteoritics, 25, 137-143. [4] Gray C. M. et al. (1973) Icarus, 20, 213-239. [5] Tatsumoto M. et al. (1976) GCA, 40, 617-634. [6] Kimura M. and Ikeda Y. (1992) Papers 17th Symp. Antarct. Meteor., 31-33. [7] Jambon A. and Carron J. P. (1976) GCA, 40, 897-903. Fig. 1, which appears here in the hard copy, shows an 87Rb-87Sr evolution diagram for chondrules from the Allende meteorite.

Nakamura, N.; Kimura, M.; Shimoda, H.; Nohda, S.

1993-07-01

410

Chemical composition, aroma evaluation, and oxygen radical absorbance capacity of volatile oil extracted from Brassica rapa cv. "yukina" used in Japanese traditional food.  

PubMed

The chemical composition of the volatile oil extracted from the aerial parts of Brassica rapa cv. "yukina" was analyzed using GC-MS, GC-PFPD, and GC-O. A total of 50 compounds were identified. The most prominent constituents were (E)-1,5-heptadiene (40.27%), 3-methyl-3-butenenitrile (25.97%) and 3-phenylpropanenitrile (12.41%). With regard to aroma compounds, 12 compounds were identified by GC-O analysis. The main aroma-active compounds were dimethyl tetrasulfide (sulphury-cabbage, FD = 64), 3-phenylpropanenitrile (nutty, FD = 64), 3-methylindole (pungent, FD = 64), and methional (potato, FD = 32). The antioxidant activity of the aroma-active compounds of the oil was determined using an oxygen radical absorbance capacity (ORAC) assay using fluorescein as the fluorescent probe. The ORAC values were found to be 785 ± 67 trolox equivalents (?mol TE/g) for B. rapa cv. "yukina" oil. The results obtained showed that the volatile oil extracted from the aerial parts is a good dietary source of antioxidants. PMID:24919480

Usami, Atsushi; Motooka, Ryota; Takagi, Ayumi; Nakahashi, Hiroshi; Okuno, Yoshiharu; Miyazawa, Mitsuo

2014-01-01

411

Multilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv 'Fortune'.  

PubMed

The genetic structure of 2n gametes and, particularly, the parental heterozygosity restitution at each locus depends on the meiotic process by which they originated, with first-division restitution and second-division restitution (SDR) being the two major mechanisms. The origin of 2n gametes in citrus is still controversial, although sexual polyploidisation is widely used for triploid seedless cultivar development. In this study, we report the analysis of 2n gametes of mandarin cv 'Fortune' by genotyping 171 triploid hybrids with 35 simple sequence repeat markers. The microsatellite DNA allele counting-peak ratios method for allele-dosage evaluation proved highly efficient in segregating triploid progenies and allowed half-tetrad analysis (HTA) by inferring the 2n gamete allelic configuration. All 2n gametes arose from the female genitor. The observed maternal heterozygosity restitution varied between 10 and 82%, depending on the locus, thus SDR appears to be the mechanism underlying 2n gamete production in mandarin cv 'Fortune'. A new method to locate the centromere, based on the best fit between observed heterozygosity restitution within a linkage group and theoretical functions under either partial or no chiasmata interference hypotheses was successfully applied to linkage group II. The maximum value of heterozygosity restitution and the pattern of restitution along this linkage group would suggest there is partial chiasma interference. The implications of such a restitution mechanism for citrus breeding are discussed. PMID:21587302

Cuenca, J; Froelicher, Y; Aleza, P; Juárez, J; Navarro, L; Ollitrault, P

2011-10-01

412

Safety and immunogenicity of 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Nigerian children: Booster dose and 2-dose catch-up regimens in the second year of life.  

PubMed

In a previous study, 3-dose primary vaccination of Nigerian infants with the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) was immunogenic for vaccine pneumococcal serotypes, with comparable tolerability between PHiD-CV and control groups. In an open-label study (ClinicalTrials.gov, NCT01153893), 68 primed children received a PHiD-CV booster dose co-administered with a diphtheria-tetanus-acellular pertussis (DTPa) booster dose at 15-21 months and 36 children unprimed for pneumococcal vaccination received two PHiD-CV catch-up doses (first dose co-administered with DTPa booster dose) at 15-21 and 17-23 months. Adverse events were recorded and immune responses were measured before and one month after vaccination. In both groups, pain was the most frequent solicited local symptom and fever was the most frequent solicited general symptom after the booster dose and each catch-up dose. Few grade 3 solicited symptoms and no vaccine-related serious adverse events were reported. After booster vaccination, for each vaccine serotype, at least 98.5% of children had an antibody concentration ? 0.2 µg/ml and at least 94.0% had an opsonophagocytic activity (OPA) titer ? 8. After 2-dose catch-up, for each vaccine serotype, at least 97.1% had an antibody concentration ? 0.2 µg/ml, except for serotypes 6B (82.9%) and 23F (88.6%), and at least 91.4% had an OPA titer ?8, except for serotypes 6B (77.4%) and 19F (85.3%). PHiD-CV induced antibody responses against protein D in both groups. In conclusion, PHiD-CV administered to Nigerian toddlers as a booster dose or 2-dose catch-up was well tolerated and immunogenic for vaccine pneumococcal serotypes and protein D. PMID:24356787

Odusanya, Olumuyiwa O; Kuyinu, Yetunde A; Kehinde, Omolara A; Shafi, Fakrudeen; François, Nancy; Yarzabal, Juan Pablo; Dobbelaere, Kurt; Rüggeberg, Jens U; Borys, Dorota; Schuerman, Lode

2014-01-01

413

Direct online HPLC-CV-AFS method for traces of methylmercury without derivatisation: a matrix-independent method for urine, sediment and biological tissue samples.  

PubMed

Mercury (Hg) is a global pollutant which occurs in different species, with methylmercury (MeHg) being the critical compound due to its neurotoxicity and bioaccumulation through the food chain. Methods for trace speciation of MeHg are therefore needed for a vast range of sample matrices, such as biological tissues, fluids, soils or sediments. We have previously developed an ultra-trace speciation method for methylmercury in water, based on a preconcentration HPLC cold vapour atomic fluorescence spectrometry (HPLC-CV-AFS) method. The focus of this work is mercury speciation in a variety of sample matrices to assess the versatility of the method. Certified reference materials were used where possible, and samples were spiked where reference materials were not available, e.g. human urine. Solid samples were submitted for commonly used digestion or extraction processes to obtain a liquid sample for injection into the analytical system. For MeHg in sediment samples, an extraction procedure was adapted to accommodate MeHg separation from high amounts of Hg(2+) to avoid an overload of the column. The recovery for MeHg determination was found to be in the range of 88-104% in fish reference materials (DOLT-2, DOLT-4, DORM-3), lobster (TORT-2), seaweed (IAEA-140/TM), sediments (ERM(®)-CC580) and spiked urine and has been proven to be robust, reliable, virtually matrix-independent and relatively cost-effective. Applications in the ultra-trace concentration range are possible using the preconcentration up to 200 mL, while for higher MeHg-containing samples, lower volumes can be applied. A comparison was carried out between species-specific isotope dilution gas chromatography inductively coupled plasma mass spectrometry (SSID-GC-ICP-MS) as the gold standard and HPLC-CV-AFS for biological tissues (liver, kidney and muscle of pilot whales), showing a slope of 1.008 and R (2)?=?0.97, which indicates that the HPLC-CV-AFS method achieves well-correlated results for MeHg in biological tissues. PMID:25318461

Brombach, Christoph-Cornelius; Gajdosechova, Zuzana; Chen, Bin; Brownlow, Andrew; Corns, Warren T; Feldmann, Jörg; Krupp, Eva M

2015-01-01

414

Graduate Council Meeting Minutes 310 Jack K. Williams Administration Building  

E-print Network

. INTA 676 International Politics of the Middle East n. INTA 687 Domestic Intelligence Operations. INTA 648 Contemporary Civil Wars i. INTA 672 East Asian Security j. INTA 673 Chinese Domestic Politics in Transition k. INTA 674 US Foreign Policy in the Persian Gulf l. INTA 675 Religion and Politics in Iran m

415

UV-B protective effect of a polyacylated anthocyanin, HBA, in flower petals of the blue morning glory, Ipomoea tricolor cv. Heavenly Blue.  

PubMed

The protective effects of polyacylated anthocyanin, heavenly blue anthocyanin (HBA), in blue flower petals of morning glory (Ipomoea tricolor cv. Heavenly Blue) against UV-B induced DNA damage were examined. We first clarified the concentration of HBA in epidermal vacuoles to be 12mM, and then constructed a UV-B irradiating apparatus resembling flower petal tissue to assess the screening effect of HBA. Monochromatic (280 and 310nm) or broad UV-B induced DNA lesions were reduced completely by the HBA filter to the same molecular numbers as those in living petal epidermis. However, diluted HBA solution and trisdeacyl HBA did not have the same reduction effect. HBA was more tolerant to solar radiation than trisdeacyl HBA. These data strongly suggest that polyacylated anthocyanins in flower petals can screen harmful UV-B efficiently. This action might be largely due to aromatic acyl residues. PMID:15727855

Mori, Mihoko; Yoshida, Kumi; Ishigaki, Yasuhito; Matsunaga, Tsukasa; Nikaido, Osamu; Kameda, Kiyoshi; Kondo, Tadao

2005-03-15

416

Age-related changes in catalase and peroxidase activities in the excised leaves of Eleusine coracana Gaertin. cv PR 202 during senescence.  

PubMed

Changes in the activities of the enzymes catalase and peroxidase were studied in the excised leaves of ragi (Eleusine coracana Gaertn. cv PR 202) plants belonging to different ages. Catalase exhibited a positive and peroxidase a negative correlation with the changes in chlorophyll. Catalase and peroxidase were negatively correlated with each other. Peroxidase exhibited an age-related drift in its activity. Kinetin could maintain the levels of chlorophyll and catalase, and also caused an increase in peroxidase activity. Both indoleacetic acid and gibberellic acid had no effect on the changes of chlorophyll but increased peroxidase activity. Catalase levels were maintained by indoleacetic acid but gibberellic acid had no effect on this enzyme. PMID:6321215

Kumar, K B; Khan, P A

1983-01-01

417

Induction of homologous low temperature and ABA-responsive genes in frost resistant (Solanum commersonii) and frost-sensitive (Solanum tuberosum cv. Bintje) potato species.  

PubMed

A DNA fragment corresponding to a low-temperature- and ABA-responsive gene (Scdhn1) was amplified by PCR from genomic DNA of a wild, frost-resistant potato species, Solanum commersonii. A homologous gene (Stdhn1) was identified in Solanum tuberosum cv. Bintje, a frost-sensitive domesticated potato cultivar. The expression of the gene was studied during low temperature and ABA treatments in both Solanum species. The analysis revealed that both low temperature and ABA lead to the accumulation of a 1 kb transcript that corresponded to the PCR fragment. The induction of the gene was relatively rapid and maximum amounts of the transcripts were detected already after 1 day and 7 h of treatment with low temperature and ABA, respectively. Previous results have shown that there is no increase in the amount of endogenous ABA in S. tuberosum during low-temperature treatment, which indicates that two independent signalling pathways lead to the induction of this gene. PMID:8616256

Baudo, M M; Meza-Zepeda, L A; Palva, E T; Heino, P

1996-01-01

418

Studies on the management of root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex of green gram, Vigna radiata cv ML-1108  

PubMed Central

Studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita–wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared to untreated inoculated plants. Analysis of data showed that carbofuran and A. indica seed powder increased plant growth and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens. PMID:16052706

Haseeb, Akhtar; Sharma, Anita; Shukla, Prabhat Kuma

2005-01-01

419

On the use of Ethephon as abscising agent in cv. Crimson Seedless table grape production: combination of Fruit Detachment Force, Fruit Drop and metabolomics.  

PubMed

The effect of 2-chloroethylphosphonic acid (Ethephon, in the following ETH) as abscising agent on cv. Crimson Seedless table grape was investigated by means of Fruit Detachment Force (FDF) and Fruit Drop (FD) analyses combined with a metabolomic study carried out by High Resolution Mass Spectrometry (HRMS) and Nuclear Magnetic Resonance (NMR) spectroscopy. The effectiveness of ETH as abscising agent was ascertained with ETH concentration ranging from 1.4 to 4.0 g/L in a two-year study. The ETH treatments caused berry drops higher than 40% and induced an increase of tartaric acid, procyanidin P2, terpenoid derivatives and peonidin-3-glucoside as well as a decrease of catechin and epicatechin. HRMS-NMR covariance analysis was carried out to correlate the fluctuations of tartaric acid NMR signals to those of MS peaks of the secondary metabolites affected by ETH treatments. PMID:25308678

Rizzuti, Antonino; Aguilera-Sáez, Luis Manuel; Gallo, Vito; Cafagna, Isabella; Mastrorilli, Piero; Latronico, Mario; Pacifico, Andrea; Matarrese, Angela Maria Stella; Ferrara, Giuseppe

2015-03-15

420

Effect of soaking and fermentation on content of phenolic compounds of soybean (Glycine max cv. Merit) and mung beans (Vigna radiata [L] Wilczek).  

PubMed

Mung beans (Vigna radiata [L] Wilczek) purchased from a Spanish company as "green soybeans", showed a different phenolic composition than yellow soybeans (Glycine max cv. Merit). Isoflavones were predominant in yellow soybeans, whereas they were completely absent in the green seeds on which flavanones were predominant. In order to enhance their health benefits, both types of bean were subjected to technological processes, such as soaking and fermentation. Soaking increased malonyl glucoside isoflavone extraction in yellow beans and produced an increase in apigenin derivatives in the green beans. Lactobacillus plantarum CECT 748?T fermentation produced an increase in the bioactivity of both beans since a conversion of glycosylated isoflavones into bioactive aglycones and an increase of the bioactive vitexin was observed in yellow and green beans, respectively. In spite of potential consumer confusion, since soybean and "green soybean" are different legumes, the health benefits of both beans were enhanced by lactic fermentation. PMID:25582183

María Landete, José; Hernández, Teresa; Robredo, Sergio; Dueñas, Montserrat; de Las Rivas, Blanca; Estrella, Isabel; Muñoz, Rosario

2015-03-01

421

Pollination increases ethylene production in Lilium hybrida cv. Brindisi flowers but does not affect the time to tepal senescence or tepal abscission.  

PubMed

In many species, pollination induces a rapid increase in ethylene production, which induces early petal senescence, petal abscission, or flower closure. Cross-pollination in Lilium hybrida cv. Brindisi resulted in a small increase in flower ethylene production. In intact plants and in isolated flowers, pollination had no effect on the time to tepal senescence or tepal abscission. When applied to closed buds of unpollinated flowers, exogenous ethylene slightly hastened the time to tepal senescence and abscission. However, exogenous ethylene had no effect when the flowers had just opened, i.e. at the time of pollination. Experiments with silver thiosulphate, which blocks the ethylene receptor, indicated that endogenous ethylene had a slight effect on the regulation of tepal senescence and tepal abscission, although only at the time the tepals were still inside buds and not in open flowers. Low ethylene-sensitivity after anthesis therefore explains why pollination had no effect on the processes studied. PMID:25462085

Pacifici, Silvia; Prisa, Domenico; Burchi, Gianluca; van Doorn, Wouter G

2014-09-01

422

Effect of Maceration Time on Free and Bound Volatiles of Red Wines from cv. Karao?lan (Vitis vinifera L.) Grapes Grown in Arapgir, Turkey.  

PubMed

Skin maceration is an essential winemaking technique that affects sensory properties and aroma of red wines. The aim of this study was to investigate aroma profiles of red wines made from cv. Karao?lan, a grape variety grown in Arapgir (Malatya, Turkey) and the effect of maceration time (5, 10, and 15 d) on free and bound aroma compounds and sensory properties. Three maceration times were applied in durations of 5, 10, and 15 d. Free and glycosidically bound aroma compounds were analyzed using gas chromatography-mass spectrometry (GC-MS) in Karao?lan wines, and the effect of different maceration times on aroma composition were evaluated. Results indicate that extended maceration time significantly influenced the aroma composition of wines both qualitatively and quantitatively. A total of 59 free and 22 bound aroma compounds were identified in the final wines. The maximum of free aroma content was achieved with 5 d of maceration, while 15 d of maceration gave the highest amount of glycosidically bound aroma compounds. From sensorial point of view, the wine produced with 10 d of maceration had the best panel score compared to wines with 5 and 15 d of maceration. This is the first study referring to the composition of aroma compounds of red wines made from cv. Karao?lan. It was concluded that this cultivar resulted in a good quality wine in terms of bound and free aroma compounds. This information of vinification practices that influence the chemical properties of wine is useful for grape growers and winemakers. PMID:25677953

Yilmaztekin, Murat; Kocabey, Nimet; Hayaloglu, Ali Adnan

2015-03-01

423

Environmental radioactivity in the South Urals 1990-1997. An international study supported by INTAS, EC and national funding.  

PubMed

Radioecological studies carried out in a joint co-operation between Russian, Ukrainian and Danish Laboratories are reported. The environmental impact of routine, discharges as well as accidental events, notably the Kyshtym accident in 1957 and the Karachay wind dispersion in 1968 have been studied. From measurements and based on model assumptions it has been estimated that the Ob river system outside Mayak, i.e. first of all the Techa and Iset rivers and their floodplains contain 0.1 PBq 90Sr, 0.3 PBq 137Cs and 0.8 TBq 239, 240Pu. The uncertainty of these estimates is a factor of 3-4. The present contamination from the Kyshtym accident outside the Mayak area is calculated to 0.1-0.5 PBq 90Sr and from the Karachay incident the contamination is 0.05-0.1 Bq 137Cs. The environmental contaminations with Pu from these two events are in the order of 1 TBq. The occurrence of 99Tc, 129I and 237Np in highly contaminated Techa river sediments collected outside Mayak is for the first time reported. PMID:14608674

Aarkrog, A; Trapeznikov, A V; Molchanova, I V; Yushkov, P I; Karavaeva, E N; Trapeznikova, V N; Pozolotina, V N; Polikarpov, G G; Dalgaard, H; Nielsen, S P

2003-01-01

424

Introducing constricted variational density functional theory in its relaxed self-consistent formulation (RSCF-CV-DFT) as an alternative to adiabatic time dependent density functional theory for studies of charge transfer transitions  

SciTech Connect

We have applied the relaxed and self-consistent extension of constricted variational density functional theory (RSCF-CV-DFT) for the calculation of the lowest charge transfer transitions in the molecular complex X-TCNE between X = benzene and TCNE = tetracyanoethylene. Use was made of functionals with a fixed fraction (?) of Hartree-Fock exchange ranging from ? = 0 to ? = 0.5 as well as functionals with a long range correction (LC) that introduces Hartree-Fock exchange for longer inter-electronic distances. A detailed comparison and analysis is given for each functional between the performance of RSCF-CV-DFT and adiabatic time-dependent density functional theory (TDDFT) within the Tamm-Dancoff approximation. It is shown that in this particular case, all functionals afford the same reasonable agreement with experiment for RSCF-CV-DFT whereas only the LC-functionals afford a fair agreement with experiment using TDDFT. We have in addition calculated the CT transition energy for X-TCNE with X = toluene, o-xylene, and naphthalene employing the same functionals as for X = benzene. It is shown that the calculated charge transfer excitation energies are in as good agreement with experiment as those obtained from highly optimized LC-functionals using adiabatic TDDFT. We finally discuss the relation between the optimization of length separation parameters and orbital relaxation in the RSCF-CV-DFT scheme.

Krykunov, Mykhaylo; Seth, Mike; Ziegler, Tom [Department of Chemistry, University of Calgary, University Drive 2500, Calgary, Alberta T2N 1N4 (Canada)] [Department of Chemistry, University of Calgary, University Drive 2500, Calgary, Alberta T2N 1N4 (Canada)

2014-05-14

425

Introducing constricted variational density functional theory in its relaxed self-consistent formulation (RSCF-CV-DFT) as an alternative to adiabatic time dependent density functional theory for studies of charge transfer transitions  

NASA Astrophysics Data System (ADS)

We have applied the relaxed and self-consistent extension of constricted variational density functional theory (RSCF-CV-DFT) for the calculation of the lowest charge transfer transitions in the molecular complex X-TCNE between X = benzene and TCNE = tetracyanoethylene. Use was made of functionals with a fixed fraction (?) of Hartree-Fock exchange ranging from ? = 0 to ? = 0.5 as well as functionals with a long range correction (LC) that introduces Hartree-Fock exchange for longer inter-electronic distances. A detailed comparison and analysis is given for each functional between the performance of RSCF-CV-DFT and adiabatic time-dependent density functional theory (TDDFT) within the Tamm-Dancoff approximation. It is shown that in this particular case, all functionals afford the same reasonable agreement with experiment for RSCF-CV-DFT whereas only the LC-functionals afford a fair agreement with experiment using TDDFT. We have in addition calculated the CT transition energy for X-TCNE with X = toluene, o-xylene, and naphthalene employing the same functionals as for X = benzene. It is shown that the calculated charge transfer excitation energies are in as good agreement with experiment as those obtained from highly optimized LC-functionals using adiabatic TDDFT. We finally discuss the relation between the optimization of length separation parameters and orbital relaxation in the RSCF-CV-DFT scheme.

Krykunov, Mykhaylo; Seth, Mike; Ziegler, Tom

2014-05-01

426

Dietary actinidin from kiwifruit (Actinidia deliciosa cv. Hayward) increases gastric digestion and the gastric emptying rate of several dietary proteins in growing rats.  

PubMed

Dietary actinidin influences the extent to which some dietary proteins are digested in the stomach, and it is hypothesized that the latter modulation will in turn affect their gastric emptying rate (GE). In this study, the effect of dietary actinidin on GE and gastric digestion of 6 dietary protein sources was determined in growing rats. Each dietary protein source [beef muscle, gelatin, gluten, soy protein isolate (SPI), whey protein isolate, and zein] was included in 2 semisynthetic diets as the sole nitrogen source. For each protein source, 1 of the 2 diets contained actinidin [76.5 U/g dry matter (DM)] in the form of ground freeze-dried green kiwifruit (Actinidia deliciosa cv. Hayward), whereas the other diet contained freeze-dried gold kiwifruit (Actinidia chinensis cv. Hort16A), which is devoid of actinidin (3.4 U/g DM). For both diets, dietary kiwifruit represented 20% of the diet on a DM basis. The real-time GE was determined in rats gavaged with a single dose of the diets using magnetic resonance spectroscopy over 150 min (n = 8 per diet). Gastric protein digestion was determined based on the free amino groups in the stomach chyme collected from rats fed the diets (n = 8 per diet) that were later killed. GE differed across the protein sources [e.g., the half gastric emptying time (T(½)) ranged from 157 min for gluten to 266 min for zein] (P < 0.05). Dietary actinidin increased the gastric digestion of beef muscle (0.6-fold), gluten (3.2-fold), and SPI (0.6-fold) and increased the GE of the diets containing beef muscle (43% T(½)) and zein (23% T(½); P < 0.05). There was an inverse correlation between gastric protein digestion and DM retained in the stomach (r = -0.67; P < 0.05). In conclusion, dietary actinidin increased gastric protein digestion and accelerated the GE for several dietary protein sources. GE may be influenced by gastric protein digestion, and dietary actinidin can be used to modulate GE and protein digestion in the stomach of some dietary protein sources but not others. PMID:24431326

Montoya, Carlos A; Hindmarsh, Jason P; Gonzalez, Lucrecia; Boland, Mike J; Moughan, Paul J; Rutherfurd, Shane M

2014-04-01

427

Effect of boiling time on chemical composition and physico-functional properties of flours from taro (Colocasia esculenta cv fouê) corm grown in Côte d'Ivoire.  

PubMed

Taro (Colocasia esculenta cv fouê) corm was subjected to different boiling times and the changes in chemical composition and physico-functional properties were investigated using standard method. The change in boiling time led to a significant (P?

Amon, Anon Simplice; Soro, René Yadé; Assemand, Emma Fernande; Dué, Edmond Ahipo; Kouamé, Lucien Patrice

2014-05-01

428

Breeding for blast-disease-resistant and high-yield Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutants using low-energy ion beams  

NASA Astrophysics Data System (ADS)

Low-energy ion beam was applied on mutation induction for plant breeding of blast-disease-resistant Thai jasmine rice (Oryza sativa L. cv. KDML 105). Seeds of the wild-type rice were bombarded in vacuum by nitrogen ion beam at energy of 60-80 keV to a beam fluence range of 2 × 1016-2 × 1017 ions/cm2. The ion-bombarded rice seeds were grown in soil for 2 weeks as transplanted rice in plastic pots at 1 seedling/pot. The seedlings were then screened for blast resistance by Pyricularia grisea inoculation with 106 spores/ml concentrations. The blast-resistant rice mutant was planted up to F6 generation with the consistent phenotypic variation. The high percentage of the blast-disease-resistant rice was analyzed with DNA fingerprint. The HAT-RAPD (high annealing temperature-random amplified polymorphic DNA) marker revealed the modified polymorphism fragment presenting in the mutant compared with wild type (KDML 105). The cDNA fingerprints were investigated and the polymorphism fragment was subcloned into pGEM-T easy vector and then sequenced. The sequence of this fragment was compared with those already contained in the database, and the fragment was found to be related to the Spotted leaf protein 11 (Spl11).

Mahadtanapuk, S.; Teraarusiri, W.; Phanchaisri, B.; Yu, L. D.; Anuntalabhochai, S.

2013-07-01

429

Pathways of nitrogen assimilation in cowpea nodules studied using /sup 15/N/sub 2/ and allopurinol. [Vigna unguiculata L. Walp. cv Vita  

SciTech Connect

In the presence of 0.5 millimolar allopurinol (4-hydroxypyrazolo (3,4-d)pyrimidine), an inhibitor of NAD:xanthine oxidoreductase (EC 1.2.3.2), intact attached nodules of cowpea (vigna unguiculata L. Walp. cv Vita 3) formed (/sup 15/N)xanthine from /sup 15/N/sub 2/ at rates equivalent to those of ureide synthesis, confirming the direct assimilation of fixed nitrogen into purines. Xanthine accumulated in nodules and was exported in increasing amounts in xylem of allopurinol-treated plants. Other intermediates of purine oxidation, de novo purine synthesis, and ammonia assimilation did not increase and, over the time course of experiments (4 hours), allopurinol had no effect on nitrogenase (EC 1.87.99.2) activity. Negligible /sup 15/N -labeling of asparagine from /sup 15/N/sub 2/ was observed, suggesting that the significant pool (up to 14 micromoles per gram of nodule fresh weight) of this amide in cowpea nodules was not formed directly from fixation but may have accumulated as a consequence of phloem delivery.

Atkins, C.A.; Storer, P.J.; Pate, J.S.

1988-01-01

430

Growth parameters and resistance against Drechslera teres of spring barley (Hordeum vulgare L. cv. Scarlett) grown at elevated ozone and carbon dioxide concentrations.  

PubMed

Spring barley ( Hordeum vulgare L. cv. Scarlett) was grown at two CO2 levels (400 vs. 700 ppm) combined with two ozone regimes (ambient vs. double ambient) in climate chambers for four weeks, beginning at seedling emergence. Elevated CO2 concentration significantly increased aboveground biomass, root biomass, and tiller number, whereas double ambient ozone significantly decreased these parameters. These ozone-induced reductions in growth parameters were strongly overridden by 700 ppm CO2. The elevated CO2 level increased C : N ratio of the leaf tissue and leaf starch content but decreased leaf protein levels. Exposure to double ambient ozone did not affect protein content and C : N ratio but dramatically increased leaf starch levels at 700 ppm CO2. Resistance against Drechslera teres (Sacc.) Shoemaker was increased in leaves grown at double ambient ozone but was less obvious at 700 ppm than at 400 ppm CO2. Constitutive activities of beta-1,3-glucanase and chitinase were significantly higher in leaves grown at double ambient ozone compared to ambient ozone levels. The sum of methanol-soluble and alkali-released cell wall-bound aromatic metabolites (i.e., C-glycosylflavones and several structurally unidentified metabolites) and lignin contents did not show any treatment-dependent differences. PMID:16388473

Plessl, M; Heller, W; Payer, H-D; Elstner, E F; Habermeyer, J; Heiser, I

2005-11-01

431

Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv. Heavenly Blue.  

PubMed

Petal color change in morning glory Ipomoea tricolor cv. Heavenly Blue, from red to blue, during the flower-opening period is due to an unusual increase in vacuolar pH (pHv) from 6.6 to 7.7 in colored epidermal cells. We clarified that this pHv increase is involved in tonoplast-localized Na+/H+ exchanger (NHX). However, the mechanism of pHv increase and the physiological role of NHX1 in petal cells have remained obscure. In this study, synchrony of petal-color change from red to blue, pHv increase, K+ accumulation, and cell expansion growth during flower-opening period were examined with special reference to ItNHX1. We concluded that ItNHX1 exchanges K+, but not Na+, with H+ to accumulate an ionic osmoticum in the vacuole, which is then followed by cell expansion growth. This function may lead to full opening of petals with a characteristic blue color. PMID:19521056

Yoshida, Kumi; Miki, Naoko; Momonoi, Kazumi; Kawachi, Miki; Katou, Kiyoshi; Okazaki, Yoshiji; Uozumi, Nobuyuki; Maeshima, Masayoshi; Kondo, Tadao

2009-01-01

432

Promoting Effect of Foliage Sprayed Zinc Sulfate on Accumulation of Sugar and Phenolics in Berries of Vitis vinifera cv. Merlot Growing on Zinc Deficient Soil.  

PubMed

The effect of foliage sprayed zinc sulfate on berry development of Vitis vinifera cv. Merlot growing on arid zone Zn-deficient soils was investigated over two consecutive seasons, 2013 and 2014. Initial zinc concentration in soil and vines, photosynthesis at three berry developmental stages, berry weight, content of total soluble solids, titratable acidity, phenolics and expression of phenolics biosynthetic pathway genes throughout the stages were measured. Foliage sprayed zinc sulfate showed promoting effects on photosynthesis and berry development of vines and the promotion mainly occurred from veraison to maturation. Zn treatments enhanced the accumulation of total soluble solids, total phenols, flavonoids, flavanols, tannins and anthocyanins in berry skin, decreasing the concentration of titratable acidity. Furthermore, foliage sprayed zinc sulfate could significantly influence the expression of phenolics biosynthetic pathway genes throughout berry development, and the results of expression analysis supported the promotion of Zn treatments on phenolics accumulation. This research is the first comprehensive and detailed study about the effect of foliage sprayed Zn fertilizer on grape berry development, phenolics accumulation and gene expression in berry skin, providing a basis for improving the quality of grape and wine in Zn-deficient areas. PMID:25648596

Song, Chang-Zheng; Liu, Mei-Ying; Meng, Jiang-Fei; Chi, Ming; Xi, Zhu-Mei; Zhang, Zhen-Wen

2015-01-01

433

Phenolic composition in grape (Vitis vinifera L. cv. Malbec) ripened with different solar UV-B radiation levels by capillary zone electrophoresis.  

PubMed

The responses of Vitis vinifera L. cv. Malbec to different solar ultraviolet-B radiation (UV-B) levels were assessed in two contrasting situations, under sunlight with full UV-B (+UV-B) and filtered UV-B (-UV-B), in three different locations at 500, 1000, and 1500 m above sea level (asl). To evaluate the effects of radiation, a simple, accurate, and rapid method for the separation and simultaneous determination of representative phenolic compounds in grape berry skins by capillary zone electrophoresis was developed. Separation was carried out in less than 20 min with 20 mM sodium tetraborate buffer containing 30% methanol, pH 9.00. The procedure is fast and reliable, and extracted grape berry skins can be directly analyzed without prior sample cleanup procedure. Berry skins from the +UV-B treatment at 1500 m asl showed the highest levels of total polyphenols anthocyanins, and resveratrol, compared with the -UV-B treatment at this altitude. PMID:18412357

Berli, Federico; D'Angelo, José; Cavagnaro, Bruno; Bottini, Rubén; Wuilloud, Rodolfo; Silva, M Fernanda

2008-05-14

434

Metabolite profiling of the short-term responses of rice leaves (Oryza sativa cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities.  

PubMed

Metabolite profiling of rice leaves (Oryza sativa cv. Ilmi) was performed to investigate the short-term responses to different light-emitting diode (LED) lights, blue (B), green (G), red (R), white (W), shade (S), by using gas chromatography-ion trap-mass spectrometry (GC-IT-MS) and ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) with multivariate analysis. Clear grouping patterns of each light-grown sample, except G and W, were shown in partial least squares-discriminant analysis (PLS-DA). Thirty-two primary metabolites and eleven secondary metabolites were selected and visualized using heatmap. Antioxidant activities of rice leaves followed the order B=W=G>R>S and isoorientin-2''-O-glucoside, isovitexin-2''-O-glucoside, isoorientin-2''-O-(6'''-?-coumaroyl)-glucoside, and isoscoparin-2''-O-glucoside showed similar relative differences and had higher Pearson's correlation coefficients than other metabolites in correlation network. According to the orthogonal projection to latent structures-discriminant analysis (OPLS-DA) between B and R, the levels of amino acids, organic acids, fatty acids, and flavonoid glycosides were relatively high in B, whereas the glucose and fructose levels were high in R. PMID:23849114

Jung, Eun Sung; Lee, Sarah; Lim, Sun-Hyung; Ha, Sun-Hwa; Liu, Kwang-Hyeon; Lee, Choong Hwan

2013-09-01

435

Antioxidant activities of trypsin inhibitor, a 33 KDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57).  

PubMed

Trypsin inhibitors (TIs), root storage proteins, were purified from sweet potato (Ipomoea batatas[L.] Lam cv. Tainong 57) roots by trypsin affinity column according to the methods of Hou and Lin (Plant Sci. 1997, 126, 11-19 and Plant Sci. 1997, 128, 151-158). A single band of 33 kDa TI was obtained by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. This purified 33 kDa TI had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. There was positive correlation between scavenging effects against DPPH (2 to 22%) and amounts of 33 kDa TI (1.92 to 46 pmol). The scavenging activities of 33 kDa TI against DPPH were calculated from linear regression to be about one-third of those of glutathione between 5 and 80 pmol. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detection, it was found that 33 kDa TI could capture hydroxyl radical, and the intensities of EPR signal were significantly decreased from 1.5 to 6 pmol of 33 kDa TI compared to those of the controls. It is suggested that 33 kDa TI, one of the sweet potato root storage proteins, may play a role as an antioxidant in roots and may be beneficial to health when it is consumed. PMID:11409996

Hou, W C; Chen, Y C; Chen, H J; Lin, Y H; Yang, L L; Lee, M H

2001-06-01

436

A peroxisomally localized acyl-activating enzyme is required for volatile benzenoid formation in a Petunia×hybrida cv. ‘Mitchell Diploid’ flower  

PubMed Central

Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petunia×hybrida cv. ‘Mitchell Diploid’ (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway intermediate by a coenzyme A (CoA) dependent, ?-oxidative scheme. Metabolic flux analysis, reverse genetics, and biochemical characterizations of key enzymes in this pathway have supported this putative concept. However, the theoretical first enzymatic reaction, which leads to the production of cinnamoyl-CoA, has only been physically demonstrated in a select number of bacteria like Streptomyces maritimus through mutagenesis and recombinant protein production. A transcript hasbeen cloned and characterized from MD flowers that shares high homology with an Arabidopsis thaliana transcript ACYL-ACTIVATING ENZYME11 (AtAAE11) and the S. maritimus ACYL-COA:LIGASE (SmEncH). In MD, the PhAAE transcript accumulates in a very similar manner as bona fide FVBP network genes, i.e. high levels in an open flower petal and ethylene regulated. In planta, PhAAE is localized to the peroxisome. Upon reduction of PhAAE transcript through a stable RNAi approach, transgenic flowers emitted a reduced level of all benzenoid volatile compounds. Together, the data suggest that PhAAE may be responsible for the activation of t-cinnamic acid, which would be required for floral volatile benzenoid production in MD. PMID:22771854

2012-01-01

437

Purification and characterization of a glucosamine-binding antifungal lectin from Phaseolus vulgaris cv. Chinese pinto beans with antiproliferative activity towards nasopharyngeal carcinoma cells.  

PubMed

A lectin has successfully been isolated from Phaseolus vulgaris cv. Chinese pinto bean using affinity chromatography, ion exchange chromatography, and gel filtration in succession, with a 15.4-fold purification. Investigation of its characteristics revealed that Chinese pinto bean lectin (CPBL) was a 58-kDa dimeric glucosamine-binding protein. Its Mg(2+)-dependent hemagglutinating activity was stable at pH 7-8 and at or below 60 °C. When the purified lectin was tested against six fungal species including Phyllosticta citriasiana, Magnaporthe grisea, Bipolans maydis, Valsa mali, Mycosphaerella arachidicola, and Setosphaeria turcica, only the mycelial growth of V. mali was reduced by 30.6 % by the lectin at 30 ?M. The lectin did not exert any discernible antiproliferative effects on breast cancer MCF-7 cells, but was able to suppress proliferation of nasopharyngeal carcinoma HONE-1 cells, with an IC50 of 17.3 ?M, as revealed by the MTT assay. Since few plant lectins demonstrate antifungal activity against V. mali, and not many others have inhibitory effects on HONE-1 cells, CPBL is a distinctive lectin which may be exploited for development into an agent against V. mali and HONE-1 cells. PMID:24114321

Ang, Andrew Si Wo; Cheung, Randy Chi Fai; Dan, Xiuli; Chan, Yau Sang; Pan, Wenliang; Ng, Tzi Bun

2014-01-01

438

Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv. Heavenly Blue  

PubMed Central

Petal color change in morning glory Ipomoea tricolor cv. Heavenly Blue, from red to blue, during the flower-opening period is due to an unusual increase in vacuolar pH (pHv) from 6.6 to 7.7 in colored epidermal cells. We clarified that this pHv increase is involved in tonoplast-localized Na+/H+ exchanger (NHX). However, the mechanism of pHv increase and the physiological role of NHX1 in petal cells have remained obscure. In this study, synchrony of petal-color change from red to blue, pHv increase, K+ accumulation, and cell expansion growth during flower-opening period were examined with special reference to ItNHX1. We concluded that ItNHX1 exchanges K+, but not Na+, with H+ to accumulate an ionic osmoticum in the vacuole, which is then followed by cell expansion growth. This function may lead to full opening of petals with a characteristic blue color. PMID:19521056

Yoshida, Kumi; Miki, Naoko; Momonoi, Kazumi; Kawachi, Miki; Katou, Kiyoshi; Okazaki, Yoshiji; Uozumi, Nobuyuki; Maeshima, Masayoshi; Kondo, Tadao

2009-01-01

439

Oxygen isotopic and chemical zoning of melilite crystals in a type A Ca-Al-rich inclusion of Efremovka CV3 chondrite  

NASA Astrophysics Data System (ADS)

Different oxygen isotopic reservoirs have been recognized in the early solar system. Fluffy type A Ca-Al-rich inclusions (CAIs) are believed to be direct condensates from a solar nebular gas, and therefore, have acquired oxygen from the solar nebula. Oxygen isotopic and chemical compositions of melilite crystals in a type A CAI from Efremovka CV3 chondrite were measured to reveal the temporal variation in oxygen isotopic composition of surrounding nebular gas during CAI formation. The CAI is constructed of two domains, each of which has a core-mantle structure. Reversely zoned melilite crystals were observed in both domains. Melilite crystals in one domain have a homogeneous 16O-poor composition on the carbonaceous chondrite anhydrous mineral (CCAM) line of ?18O = 5-10‰, which suggests that the domain was formed in a 16O-poor oxygen isotope reservoir of the solar nebula. In contrast, melilite crystals in the other domain have continuous variations in oxygen isotopic composition from 16O-rich (?18O = -40‰) to 16O-poor (?18O = 0‰) along the CCAM line. The oxygen isotopic composition tends to be more 16O-rich toward the domain rim, which suggests that the domain was formed in a variable oxygen isotope reservoir of the solar nebula. Each domain of the type A CAI has grown in distinct oxygen isotope reservoir of the solar nebula. After the domain formation, domains were accumulated together in the solar nebula to form a type A CAI.

Kawasaki, Noriyuki; Sakamoto, Naoya; Yurimoto, Hisayoshi

2012-12-01

440

Developmental patterns of emission of scent compounds and related gene expression in roses of the cultivar Rosa x hybrida cv. 'Yves Piaget'.  

PubMed

2-Phenylethanol (2PE) and 3,5-dimethoxytoluene (DMT) are characteristic scent compounds in specific roses such as Rosa x hybrida cv. 'Yves Piaget'. We analyzed the endogenous concentrations and emission of 2PE and DMT during the unfurling process in different floral organs, as well as changes in transcript levels of the two key genes, PAR and OOMT2. The emission of both 2PE and DMT increased during floral development to reach peaks at the fully unfurled stage. The relative transcripts of PAR and OOMT2 also increased during floral development. Whereas the maximum for OOMT2 was found at the fully unfurled stage (stage 4), similar expression levels of PAR were detected at stage 4 and the senescence stage (stage 6). The results demonstrate a positive correlation between the expression levels of PAR and OOMT2 and the emission of 2PE and DMT. In addition, endogenous volatiles and relative transcripts showed tissue- and development-specific patterns. PMID:25576838

Chen, Xiaomin; Baldermann, Susanne; Cao, Shuyan; Lu, Yao; Liu, Caixia; Hirata, Hiroshi; Watanabe, Naoharu

2015-02-01

441

The photosynthetic and stomatal response of Medicago sativa cv. saranac to free-air CO{sub 2} enrichment (F.A.C.E.) and nitrogen  

SciTech Connect

Plots of Medicago sativa cv. saranac were grown in the field at ambient (355 {mu}mol CO{sub 2} mol{sup -1} air) or elevated (600{mu}mol CO{sub 2} mol{sup -1} air) CO{sub 2} concentrations. High (200kg yr{sup -1}) or low (20kg yr{sup -1}) nitrogen levels were applied to two isogeneic lines, one able and one unable to use nitrogen fixing bacteria. Plants were in the second year of field growth. Exposure to elevated CO{sub 2} was via a Free-Air CO{sub 2} Enrichment System (FACE). Elevated CO{sub 2} increased diurnal assimilation by between 12% and 92%. Analysis of A/C{sub i} responses showed that effective nitrogen fertilisation was more important to rubisCO and RuBP activity than elevated CO{sub 2}. No acclimation was consistently observed. Leaves lower down the canopy were found to have lower Vc{sub max} and J{sub max} values, though age may be the cause of the latter effect. FACE conditions have only a small effect on these responses. There was some evidence found for the down-regulation of photosynthesis in the late afternoon. The FACE conditions had no affect on stomatal density but did increase epidermal cell density.

Bridson, N.P.

1996-08-01

442

The F-box protein COI1 functions upstream of MYB305 to regulate primary carbohydrate metabolism in tobacco (Nicotiana tabacum L. cv. TN90)  

PubMed Central

Jasmonate (JA) plays an important role in regulating plant male fertility and secondary metabolism, but its role in regulating primary metabolism remains unclear. The F-box protein CORONATINE INSENSITIVE 1 (COI1) is a critical component of the JA receptor, and mediates JA-signalling by targeting JASMONATE ZIM-domain (JAZ) proteins for proteasomal degradation in response to JA perception. Here, we found that RNA interference-mediated knockdown of NtCOI1 in tobacco (Nicotiana tabacum L. cv. TN90) recapitulated many previously observed phenotypes in coi1 mutants, including male sterility, JA insensitivity, and loss of floral anthocyanin production. It also affected starch metabolism in the pollen, anther wall, and floral nectary, leading to pollen abortion and loss of floral nectar. Transcript levels of genes encoding starch metabolism enzymes were significantly altered in the pollen, anther wall, and floral nectary of NtCOI1-silenced tobacco. Changes in leaf primary metabolism were also observed in the NtCOI1-silenced tobacco. The expression of NtMYB305, an orthologue of MYB305 previously identified as a flavonoid metabolic regulator in Antirrhinum majus flowers and as a floral-nectar regulator mediating starch synthesis in ornamental tobacco, was extremely downregulated in NtCOI1-silenced tobacco. These findings suggest that NtCOI1 functions upstream of NtMYB305 and plays a fundamental role in coordinating plant primary carbohydrate metabolism and correlative physiological processes. PMID:24604735

Zhang, Hongbo

2014-01-01

443

Germacrene C synthase from Lycopersicon esculentum cv. VFNT Cherry tomato: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene?cyclase  

PubMed Central

Germacrene C was found by GC-MS and NMR analysis to be the most abundant sesquiterpene in the leaf oil of Lycopersicon esculentum cv. VFNT Cherry, with lesser amounts of germacrene A, guaia-6,9-diene, germacrene B, ?-caryophyllene, ?-humulene, and germacrene D. Soluble enzyme preparations from leaves catalyzed the divalent metal ion-dependent cyclization of [1-3H]farnesyl diphosphate to these same sesquiterpene olefins, as determined by radio-GC. To obtain a germacrene synthase cDNA, a set of degenerate primers was constructed based on conserved amino acid sequences of related terpenoid cyclases. With cDNA prepared from leaf epidermis-enriched mRNA, these primers amplified a 767-bp fragment that was used as a hybridization probe to screen the cDNA library. Thirty-one clones were evaluated for functional expression of terpenoid cyclase activity in Escherichia coli by using labeled geranyl, farnesyl, and geranylgeranyl diphosphates as substrates. Nine cDNA isolates expressed sesquiterpene synthase activity, and GC-MS analysis of the products identified germacrene C with smaller amounts of germacrene A, B, and D. None of the expressed proteins was active with geranylgeranyl diphosphate; however, one truncated protein converted geranyl diphosphate to the monoterpene limonene. The cDNA inserts specify a deduced polypeptide of 548 amino acids (Mr = 64,114), and sequence comparison with other plant sesquiterpene cyclases indicates that germacrene C synthase most closely resembles cotton ?-cadinene synthase (50% identity). PMID:9482865

Colby, Sheila M.; Crock, John; Dowdle-Rizzo, Barbara; Lemaux, Peggy G.; Croteau, Rodney

1998-01-01

444

Arbuscular mycorrhizal fungi enhance fruit growth and quality of chile ancho (Capsicum annuum L. cv San Luis) plants exposed to drought.  

PubMed

The effect of arbuscular mycorrhizal fungi (AMF) and drought on fruit quality was evaluated in chile ancho (Capsicum annuum L. cv San Luis). AMF treatments were (1) Glomus fasciculatum (AMFG), (2) a fungal species consortium from the forest "Los Tuxtla" in Mexico (AMFT), (3) a fungal species consortium from the Sonorian desert in Mexico (AMFD), and (4) a noninoculated control (NAMF). Plants were exposed to a 26-day drought cycle. Fruit quality was determined by measuring size (length, width, and pedicel length), color, chlorophyll, and carotenoid concentration. Under nondrought conditions, AMFG produced fruits that were 13% wider and 15% longer than the NAMF treatment. Under nondrought conditions, fruit fresh weight was 25% greater in the AMFG treatment compared to the NAMF. Under drought, fruits in the AMFT and AMFD treatments showed fresh weights similar to those in the NAMF treatment not subjected to drought. Fruits of the AMFG treatment subjected to drought showed the same color intensity and chlorophyll content as those of the nondroughted NAMF treatment and carotenoid content increased 1.4 times compared to that in the NAMF not exposed to drought. It is interesting to note that fruits in the AMFD treatment subjected to drought and the NAMF treatment not exposed to drought reached the same size. AMFD treatment increased the concentration of carotenes (1.4 times) under nondrought conditions and the concentration of xanthophylls (1.5 times) under drought when compared to the nondroughted NAMF treatment. PMID:16741758

Mena-Violante, Hortencia G; Ocampo-Jiménez, Omar; Dendooven, Luc; Martínez-Soto, Gerardo; González-Castañeda, Jaquelina; Davies, Fred T; Olalde-Portugal, Víctor

2006-06-01

445

Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and desiccation in Spinacia oleracea L. cv. Bloomsdale: possible role in stress tolerance.  

PubMed

Osmopriming improves seed germination performance as well as stress tolerance. To understand the biochemistry of osmopriming-induced seed stress tolerance, we investigated dehydrin (DHN) accumulation patterns at protein and transcript level (determined by immunoblotting and qPCR) during priming, and subsequent germination under optimal and stress conditions (i.e. chilling and desiccation) in spinach (Spinacia oleracea L. cv. Bloomsdale) seeds. Our data indicate enhanced germination performance of primed seeds is accompanied by increased accumulation of three dehydrin-like proteins (DLPs): 30, 26, and 19-kD. Moreover, 30, 26 and 19-kD DLPs that first only transiently accumulated during priming re-accumulated in response to stresses, suggesting an evidence for 'cross-tolerance', which is initially induced by priming and later recruited during post-priming germination under stresses. Study with CAP85, a spinach DHN, corroborates above observations at the gene-expression and protein accumulation level. Additionally, our results suggest that during seed germination and seedling establishment, CAP85 expression may be regulated by the interplay of two factors: seedling development and stress responses. In conclusion, our data suggest that 30, 26, and 19-kD dehydrin-like proteins and CAP85 may be used as potential biochemical/molecular markers for priming-induced stress tolerance in 'Bloomsdale' spinach. PMID:22195574

Chen, Keting; Fessehaie, Anania; Arora, Rajeev

2012-02-01

446

Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901  

PubMed Central

Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit. PMID:23615464

Zhu, Xiaoyan; Luo, Fenglei; Zheng, Yixiong; Zhang, Jiukai; Huang, Jianzhen; Sun, Chongde; Li, Xian; Chen, Kunsong

2013-01-01

447

Identification, Synthesis, and Characterization of Novel Sulfur-Containing Volatile Compounds from the In-Depth Analysis of Lisbon Lemon Peels (Citrus limon L. Burm. f. cv. Lisbon).  

PubMed

Lemons (Citrus limon) are a desirable citrus fruit grown and used globally in a wide range of applications. The main constituents of this sour-tasting fruit have been well quantitated and characterized. However, additional research is still necessary to better understand the trace volatile compounds that may contribute to the overall aroma of the fruit. In this study, Lisbon lemons (C. limon L. Burm. f. cv. Lisbon) were purchased from a grove in California, USA, and extracted by liquid-liquid extraction. Fractionation and multidimensional gas chromatography-mass spectrometry were utilized to separate, focus, and enhance unidentified compounds. In addition, these methods were employed to more accurately assign flavor dilution factors by aroma extract dilution analysis. Numerous compounds were identified for the first time in lemons, including a series of branched aliphatic aldehydes and several novel sulfur-containing structures. Rarely reported in citrus peels, sulfur compounds are known to contribute significantly to the aroma profile of the fruit and were found to be aroma-active in this particular study on lemons. This paper discusses the identification, synthesis, and organoleptic properties of these novel volatile sulfur compounds. PMID:25639384

Cannon, Robert J; Kazimierski, Arkadiusz; Curto, Nicole L; Li, Jing; Trinnaman, Laurence; Ja?czuk, Adam J; Agyemang, David; Da Costa, Neil C; Chen, Michael Z

2015-02-25

448

Repression of Acetolactate Synthase Activity through Antisense Inhibition (Molecular and Biochemical Analysis of Transgenic Potato (Solanum tuberosum L. cv Desiree) Plants).  

PubMed Central

Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, valine, and isoleucine, is the biochemical target of different herbicides. To investigate the effects of repression of ALS activity through antisense gene expression we cloned an ALS gene from potato (Solanum tuberosum L. cv Desiree), constructed a chimeric antisense gene under control of the cauliflower mosaic virus 35S promoter, and created transgenic potato plants through Agrobacterium tumefaciens-mediated gene transfer. Two regenerants revealed severe growth retardation and strong phenotypical effects resembling those caused by ALS-inhibiting herbicides. Antisense gene expression decreased the steady-state level of ALS mRNA in these plants and induced a corresponding decrease in ALS activity of up to 85%. This reduction was sufficient to generate plants almost inviable without amino acid supplementation. In both ALS antisense and herbicide-treated plants, we could exclude accumulation of 2-oxobutyrate and/or 2-aminobutyrate as the reason for the observed deleterious effects, but we detected elevated levels of free amino acids and imbalances in their relative proportions. Thus, antisense inhibition of ALS generated an in vivo model of herbicide action. Furthermore, expression of antisense RNA to the enzyme of interest provides a general method for validation of potential herbicide targets. PMID:12228373

Hofgen, R.; Laber, B.; Schuttke, I.; Klonus, A. K.; Streber, W.; Pohlenz, H. D.

1995-01-01

449

Effects of modified atmosphere packing and honey dip treatments on quality maintenance of minimally processed grape cv. Razaki (V. vinifera L.) during cold storage.  

PubMed

Increasing pressure in food conservation sector to replace chemical applications has urged researchers to focus on studying new strategies of extending the postharvest life of produces. In such efforts, numerous materials have been tested for their effectiveness as well as suitability in organic consumption. In this study, effects of modified atmosphere packing (MAP) and honey solution dip on maintenance of quality of minimally processed table grape cv. Razaki were investigated. During the storage at 0 °C with relative humidity of 90%, MAP, honey dip, and their combined applications significantly retarded the weight loss of berries that retained about 2 mm of cap stem. Soluble solid contents of all berries slightly increased, while their acid amounts decreased, resulting in consecutive rises of maturity index. With respect to the sensory score, calculated as mean of ten panelists, honey treatment alone was ranked the highest while control berries had significantly lower value. Overall, MAP, honey solution dip or their combination significantly maintained the general quality of minimally processed grape by delaying quality loss and berry decay. Therefore, honey solution dip yielded promising results to use as an edible organic coating barrier to moisture and resist to water vapor diffusion during the cold storage, offering a good adherence to berry surface. PMID:23572752

Sab?r, Ali; Sab?r, Ferhan K; Kara, Zeki

2011-06-01

450

Heat shock transcription factors expression during fruit development and under hot air stress in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit.  

PubMed

Heat shock transcription factors (Hsfs) play a role in plant responses to stress. Citrus is an economically important fruit whose genome has been fully sequenced. So far, no detailed characterization of the Hsf gene family is available for citrus. A genome-wide analysis was carried out in Citrus clementina to identify Hsf genes, named CcHsfs. Eighteen CcHsfs were identified and classified into three main clades (clades A, B and C) according to the structural characteristics and the phylogenetic comparison with Arabidopsis and tomato. MEME motif analysis highlighted the conserved DBD and HR-A/B domains, which were similar to Hsf protein structures in other species. Gene expression analysis in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit identified 14 Hsf genes, named CrHsf, as important candidates for a role in fruit development and ripening, and showed seven genes to be expressed in response to hot air stress. CrHsfB2a and CrHsfB5 were considered to be important regulators of citrate content and showed variation in both developmentally-related and hot air-triggered citrate degradation processes. In summary, the data obtained from this investigation provides the basis for further study to dissect Hsf function during fruit development as well as in response to heat stress and also emphasizes the potential importance of CrHsfs in regulation of citrate metabolism in citrus fruit. PMID:25596345

Lin, Qiong; Jiang, Qing; Lin, Juanying; Wang, Dengliang; Li, Shaojia; Liu, Chunrong; Sun, Chongde; Chen, Kunsong

2015-04-01

451

Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway.  

PubMed

The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7 ?g/g dry weight (DW) and a maximum of 31.3 ?g/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3 ?g/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag. PMID:24530945

Ritala, Anneli; Dong, Lemeng; Imseng, Nicole; Seppänen-Laakso, Tuulikki; Vasilev, Nikolay; van der Krol, Sander; Rischer, Heiko; Maaheimo, Hannu; Virkki, Arho; Brändli, Johanna; Schillberg, Stefan; Eibl, Regine; Bouwmeester, Harro; Oksman-Caldentey, Kirsi-Marja

2014-04-20

452

The sequence of change within the photosynthetic apparatus of wheat following short-term exposure to ozone. [Triticum aestivum L. cv Avalon  

SciTech Connect

The basis of inhibition of photosynthesis by single acute O{sub 3} exposures was investigated in vivo using analyses based on leaf gas exchange measurements. The fully expanded second leaves of wheat plants (Triticum aestivum L. cv Avalon) were fumigated with either 200 or 400 nanomoles per mole O{sub 3} for between 4 and 16 hours. This reduced significantly the light-saturated rate of Co{sub 2} uptake and was accompanied by a parallel decrease in stomatal conductance. However, the stomatal limitation only increased significantly during the first 8 hours of exposure to 400 nanomoles per mole O{sub 3}; no significant increase occurred for any of the other treatments. Analysis of the response of CO{sub 2} uptake to the internal Co{sub 2} concentration implied that the predominant factor responsible for the reduction in light-saturated CO{sub 2} uptake was a decrease in the efficiency of carboxylation. At saturating concentrations of Co{sub 2}, photosynthesis was inhibited by no more than 22% after 16 hours, indicating that the capacity for regeneration of ribulose bisphosphate was less susceptible to O{sub 3}. Ozone fumigations also had a less pronounced effect on light-limited photosynthesis. The photochemical efficiency of photosystem II estimated from the ratio of variable to maximum chlorophyll fluorescence and the atrazine-binding capacity of isolated thylakoids demonstrated that photochemical reactions were not responsible for the initial inhibition of CO{sub 2} uptake.

Farage, P.K.; Long, S.P.; Baker, N.R. (Univ. of Essex (England)); Lechner, E.G. (Univ. of Vienna (Austria))

1991-02-01

453

Blockade of TNF in vivo using cV1q antibody reduces contractile dysfunction of skeletal muscle in response to eccentric exercise in dystrophic mdx and normal mice  

Microsoft Academic Search

This study evaluated the contribution of the pro-inflammatory cytokine, tumour necrosis factor (TNF) to the severity of exercise-induced muscle damage and subsequent myofibre necrosis in mdx mice. Adult mdx and non-dystrophic C57 mice were treated with the mouse-specific TNF antibody cV1q before undergoing a damaging eccentric contraction protocol performed in vivo on a custom built mouse dynamometer. Muscle damage was

A. T. Piers; T. Lavin; H. G. Radley-Crabb; A. J. Bakker; M. D. Grounds; G. J. Pinniger

2011-01-01

454

Impact of different combined doses of fertilizers with plant growth regulators on growth, yield attributes and yield of mustard ( Brassica campestris cv. B 9 ) under old alluvial soil of Burdwan, West Bengal, India  

Microsoft Academic Search

Field experiments were conducted with mustard (Brassica campestris cv. B9) consecutively for three years (2005, 2006 and 2007) in the Crop Research and Seed Multiplication Farm of Burdwan University,\\u000a Burdwan, West Bengal, India. In the first year, varietal screening of mustard under recommended dose of chemical fertilizer\\u000a (100:50:50) were performed with seven mustard varieties during November 2005 to February 2006.

Arnab Banerjee; Jayanta Kumar Datta; Naba Kumar Mondal

2010-01-01

455

Transmission infrared spectra (2-25 ?m) of carbonaceous chondrites (CI, CM, CV-CK, CR, C2 ungrouped): Mineralogy, water, and asteroidal processes  

NASA Astrophysics Data System (ADS)

In this work, infrared transmission spectra (2-25 ?m range, 5000-400 cm-1) of 40 carbonaceous chondrites were analyzed (21 CMs, 5 CVs, 6 CRs, 3 CKs, 3 C2s and 2 CIs). All these meteorite groups are known to have experienced significant aqueous alteration (except the CKs). These IR measurements provide information about the parent body processes experienced, as well as spectra for comparison with observations of Solar System small bodies and possibly with astronomical observations of accretion and debris disks. This study reveals that each meteorite group appears to have specific signatures in the measured IR spectral range. In the case of the CI and CM groups, results show a variability in the shape of the silicate features that can be related to the evolution of the mineralogy with increasing extent of aqueous alteration extent as described by several authors with other techniques. This evolution of the silicate feature can be seen in the variation in the relative intensities of olivine and phyllosilicate IR features. The variability in the silicate features is correlated with the intensity of an -OH related absorption at 3-?m, which can be used for the classification of the meteorites according to the level of hydration. Interestingly, in the case of CM chondrites, for which the mineralogy is expected to be dominated by phyllosilicates (serpentine mostly), the shape of the silicate absorption resembles that of an amorphous silicate, with a broad and symmetric 10-?m band, unlike terrestrial phyllosilicates. The CV and CK groups have IR spectra that are dominated by olivine absorption. From this feature, it is possible to determine average Mg numbers for the olivine. For the CVs, the olivine Mg numbers appear to decrease in the order Kaba-Grosnaja-Vigarano-Mokoia-Allende. This trend is likely related to the long duration of metamorphism experienced by these samples and the chemical re-equilibration between chondritic components. In the case of CK chondrites, the inferred bulk Mg# of olivine is 67 (±1), and no variation is observed between the three studied samples, which is likely related to their high degree of equilibration. The 6 CR chondrites show the most variability in their IR spectra, from CM-like spectra in the case of the CR1 Grosvenor Mountains (GRO) 95577 to CV-like spectra for Roberts Massif (RBT) 04133 and Graves Nunataks (GRA) 06100 (one of them being most probably misclassified). Spectra of the remaining CRs show mixtures of various silicate component. Finally, these spectra can be used for comparison with emission spectra of fine-grained asteroid surfaces and dust-rich cometary tails. In the case of Tempel 1, the only group of CC that matches the observed feature around 10-?m region is the CR group. The spectral comparison shows some striking similarities between CRs and Tempel 1 dust. A genetic link between CR2 and comets is not proven, but mineralogical similarities are suggested from the IR spectra.

Beck, P.; Garenne, A.; Quirico, E.; Bonal, L.; Montes-Hernandez, G.; Moynier, F.; Schmitt, B.

2014-02-01

456

Catabolism of (+/-)-abscisic acid by excised leaves of Hordeum vulgare L. cv Dyan and its modification by chemical and environmental factors  

SciTech Connect

Excised light-grown leaves and etiolated leaves of Hordeum vulgare L. cv Dyan catabolized applied (+/-)-(2-/sup 14/C)abscisic acid ((+/-)-(2-/sup 14/C)ABA) to phaseic acid (PA), dihydrophaseic acid (DPA), and 2'-hydroxymethyl ABA (2'-HMABA). Identification of these catabolites was made by microchemical methods and by combined capillary gas chromatography-mass spectrometry (GC-MS) following high dose feeds of nonlabeled substrate to leaves. Circular dichroism analysis revealed that 2'-HMABA was derived from the (-) enantiomer of ABA. Refeeding studies were used to confirm the catabolic route. The methyl ester of (+/-)-(2/sup 14/C)-ABA was hydrolyzed efficiently by light-grown leaves of H. vulgare. Leaf age played a significant role in (+/-)-ABA catabolism, with younger leaves being less able than their older counterparts to catabolize this compound. The catabolism of (+/-)-ABA was inhibited markedly in water-stressed Hordeum leaves which was characterized by a decreased incorporation of label into 2'-HMABA, DPA, and conjugates. The specific, mixed function oxidase inhibitor, ancymidol, did not inhibit, dramatically (+/-)-ABA catabolism in light-grown leaves of Hordeum whereas the 80s ribosome, translational inhibitor, cycloheximide, inhibited this process markedly. The 70s ribosome translational inhibitors, lincomycin and chloramphenicol, were less effective than cycloheximide in inhibiting (+/-)-ABA catabolism, implying that cytoplasmic protein synthesis is necessary for the catabolism of (+/-)-ABA in Hordeum leaves whereas chloroplast protein synthesis plays only a minor role. This further suggests that the enzymes involved in (+/-)-ABA catabolism in this plant are cytoplasmically synthesized and are turned-over rapidly, although the enzyme responsible for glycosylating (+/-)-ABA itself appeared to be stable.

Cowan, A.K.; Railton, I.D.

1987-05-01

457

Rare Earth Element Transfer from Soil to Navel Orange Pulp (Citrus sinensis Osbeck cv. Newhall) and the Effects on Internal Fruit Quality.  

PubMed

The effects of soil rare earth element (REE) on navel orange quality and safety in rare earth ore areas have gained great attention. This study investigated the transfer characteristics of REE from soil to navel orange pulp (Citrus sinensis Osbeck cv. Newhall) and examined the effects of soil REE on internal fruit quality in Xinfeng County, Jiangxi province, China. Path analysis showed that soil REE, pH, cation exchange capacity (CEC), and Fe oxide (Feox) significantly affected pulp REE concentrations. A Freundlich-type prediction model for pulp REE was established: log[REEpulp] = -1.036 + 0.272 log[REEsoil] - 0.056 pH - 0.360 log[CEC] + 0.370 log[Feox] (n = 114, R2 = 0.60). From the prediction model, it was inferred that even when soil REE and Feox were as high as 1038 mg kg-1 and 96.4 g kg-1, respectively, and pH and CEC were as low as 3.75 and 5.08 cmol kg-1, respectively, pulp REE concentrations were much lower than the food limit standard. Additionally, soil REE levels were significantly correlated with selected fruit quality indicators, including titratable acidity (r = 0.52, P < 0.01), total soluble solids (r = 0.48, P < 0.01) and vitamin C (r = 0.56, P < 0.01). Generally, under routine methods of water and fertilization management, the cultivation of navel oranges in rare earth ore areas of south China with soil REE ranging from 38.6 to 546 mg kg-1 had improved in internal fruit quality. PMID:25806821

Cheng, Jinjin; Ding, Changfeng; Li, Xiaogang; Zhang, Taolin; Wang, Xingxiang

2015-01-01

458

QTL analysis for grain quality traits in 2 BC2F2 populations derived from crosses between Oryza sativa cv Swarna and 2 accessions of O. nivara.  

PubMed

The appearance and cooking quality of rice determine its acceptability and price to a large extent. Quantitative trait loci (QTLs) for 12 grain quality traits were mapped in 2 mapping populations derived from Oryza sativa cv Swarna × O. nivara. The BC(2)F(2) population of the cross Swarna × O. nivara IRGC81848 (population 1) was evaluated during 2005 and that from Swarna × O. nivara IRGC81832 (population 2) was evaluated during 2006. Linkage maps were constructed using 100 simple sequence repeat (SSR) markers in population 1 and 75 SSR markers in population 2. In all, 21 QTLs were identified in population 1 (43% from O. nivara) and 37 in population 2 (38% QTLs from O. nivara). The location of O. nivara-derived QTLs mp1.2 for milling percent, kw6.1 for kernel width, and klac12.1 for kernel length after cooking coincided in the 2 populations and appear to be useful for Marker Assisted Selection (MAS). Four QTLs for milling percent, 1 QTL each for amylose content, water uptake, elongation ratio, 2 QTLs for kernel width, and 3 QTLs for gel consistency, each explained more than 20% phenotypic variance. Three QTL clusters for grain quality traits were close to the genes/QTLs for shattering and seed dormancy. QTLs for 4 quality traits were associated with 5 of the 7 major yield QTLs reported in the same 2 mapping populations. Useful introgression lines have been developed for several agronomic traits. It emerges that 40% O. nivara alleles were trait enhancing in both populations, and QTLs for grain quality overlapped with yield meta-QTLs and QTLs for dormancy and seed shattering. PMID:22312119

Swamy, B P Mallikarjuna; Kaladhar, K; Shobha Rani, N; Prasad, G S V; Viraktamath, B C; Reddy, G Ashok; Sarla, N

2012-01-01

459

Control of rotting and browning of Longan fruit cv. Biew Kiew after harvested by sulphur dioxide treatment under various storage temperatures.  

PubMed

The experiment of Longan fruit cv. Biew Kiew, untreated (control) and treated with SO2 treatment were stored under 2 +/- 2 and 7 +/- 2 degrees C for 0, 2, 4, 6 and 8 weeks were studied. The treatment of fresh longan fruit with SO2 fumigation combined with the suitable storage condition improved the overall longan fruit quality, especially on inner and outer peel tissue and aril color than no SO2 treatment. Treatment stabilizes peel color with no subsequent loss of color during storage (fruit color were bright-yellowish color). When the fruit showed during SO2 treatment, increasing of storage duration and temperatures, the dark color of inner and outer peel of longan fruit was appeared, this was correlated with the increasing of PPO activity. The activity of PPO enzyme in control fruit (no SO2 treatment) gradually lower than SO2 treatments. Fruit exposed to cool storage temperature (2 degrees C) exhibited a lower PPO enzymatic activity compared to those kept in high storage temperature (7 degrees C). Moreover, PPO enzymatic activity significantly increased over the storage durations The additional SO2 treatment no subsequent loss of weight of longan fruit during storage. However, the sulphite residues could detect immediately after SO2 treatment in all part of longan fruit, especially on aril tissue. The SEM evaluation found that the surface cracking was also impair the physiological function of the cuticle and increasing water permeability, which may cause water soaking at the inner side of the peel. The injured cell would accelerate the oxidation of phenolic substances and the oxidative products resulted in dark color of inner and outer peel. PMID:20180317

Chitbanchong, W; Sardsud, V; Whangchai, K; Koslanund, R; Thobunluepop, P

2009-11-15