Science.gov

Sample records for cv cobriza inta

  1. The INTA AHS system

    NASA Astrophysics Data System (ADS)

    Fernández-Renau, Alix; Gómez, José Antonio; de Miguel, Eduardo

    2005-10-01

    The Remote Sensing Laboratory at INTA owns and operates an 80-band airborne hyperspectral line-scanner radiometer, alias AHS. This instrument is based on previous airborne hyperspectral scanners as MIVIS and MAS. This instrument has been installed in the INTÁs aircraft (CASA C-212), and integrated with a GPS/INS. The acquired imagery is processed and archived by INTA. For this purpose, a processing chain has been implemented at the INTA premises in Madrid. In this chain, raw data (level 0 product) is transformed to at-sensor radiance (level 1b) and later to geolocated at-sensor radiance (level 1c). Other processing levels, as atmospherically corrected reflectance, brightness temperature or surface emissivity could also be produced. The radiometric calibration is based on laboratory measurements using an integrating sphere for the reflective bands, and on in-flight blackbodies measurements for the thermal bands. The geolocation procedure is based on processing of GPS/INS data synchronized with the imagery collection. Finally, direct parametric georeferencing is achieved by means of the commercial software PARGE. The resulting system is available to the international remote sensing community through specific agreements (contractual or based on joint collaborations). As an example of the use of this system, an on-going project to evaluate water stress in olives in southern Spain is presented. In this project, high resolution thermal radiometry is used to evaluate tree temperature, while reflective bands are used for identifying individual trees and for simultaneous monitoring of plant health.

  2. CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains

    PubMed Central

    Wright, Patrick R.; Georg, Jens; Mann, Martin; Sorescu, Dragos A.; Richter, Andreas S.; Lott, Steffen; Kleinkauf, Robert; Hess, Wolfgang R.; Backofen, Rolf

    2014-01-01

    CopraRNA (Comparative prediction algorithm for small RNA targets) is the most recent asset to the Freiburg RNA Tools webserver. It incorporates and extends the functionality of the existing tool IntaRNA (Interacting RNAs) in order to predict targets, interaction domains and consequently the regulatory networks of bacterial small RNA molecules. The CopraRNA prediction results are accompanied by extensive postprocessing methods such as functional enrichment analysis and visualization of interacting regions. Here, we introduce the functionality of the CopraRNA and IntaRNA webservers and give detailed explanations on their postprocessing functionalities. Both tools are freely accessible at http://rna.informatik.uni-freiburg.de. PMID:24838564

  3. CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains.

    PubMed

    Wright, Patrick R; Georg, Jens; Mann, Martin; Sorescu, Dragos A; Richter, Andreas S; Lott, Steffen; Kleinkauf, Robert; Hess, Wolfgang R; Backofen, Rolf

    2014-07-01

    CopraRNA (Comparative prediction algorithm for small RNA targets) is the most recent asset to the Freiburg RNA Tools webserver. It incorporates and extends the functionality of the existing tool IntaRNA (Interacting RNAs) in order to predict targets, interaction domains and consequently the regulatory networks of bacterial small RNA molecules. The CopraRNA prediction results are accompanied by extensive postprocessing methods such as functional enrichment analysis and visualization of interacting regions. Here, we introduce the functionality of the CopraRNA and IntaRNA webservers and give detailed explanations on their postprocessing functionalities. Both tools are freely accessible at http://rna.informatik.uni-freiburg.de. PMID:24838564

  4. Photometric follow-up of transiting exoplanets with the INTA-CAB robotic telescope

    NASA Astrophysics Data System (ADS)

    Ullán, A.; Eibe, M. T.; Cuesta, L.; Pérez-Verde, A.; Navas, J.

    2013-05-01

    We present some photometric results obtained by our group as part of a photometric follow-up of transiting exoplanets carried out with the INTA-CAB 50-cm robotic telescope at Calar Alto Observatory (Almería, Spain). We show light curves of WASP-10, HATP-20 and HATP-32 and, after that, we fit our results with theoretical models in order to obtain some parameters as the central times of transits, depths and the transit durations of this extrasolar planets.

  5. Report on upgrade of NIS magnetic observatories by the INTAS infrastructure action CRENEGON

    NASA Astrophysics Data System (ADS)

    Rasson, J. L.; Potapov, A. S.; Korepanov, V. E.; Khomutov, S. Y.; Krasnov, V. M.; Bitterly, J.

    2006-06-01

    We present the overall strategy used to upgrade 9 former USSR observatories to INTERMAGNET standards. The following aspects are discussed: application for funding to the INTAS European ad-hoc agency, choice of observatories to upgrade, choice of the instrumentation to purchase and to install, calibration of the instrumentation and used standards, back-ups, installation of the instrumentation and observatory real estate upgrade, acquisition and communication, training of the observers and documentation, evaluation of the upgrades and management of the project. Several observatories were successfully upgraded and we expect their INTERMAGNET certification soon.

  6. Freiburg RNA Tools: a web server integrating IntaRNA, ExpaRNA and LocARNA

    E-print Network

    Smith, Cameron

    The Freiburg RNA tools web server integrates three tools for the advanced analysis of RNA in a common web-based user interface. The tools IntaRNA, ExpaRNA and LocARNA support the prediction of RNA–RNA interaction, exact ...

  7. Freiburg RNA Tools: a web server integrating IntaRNA, ExpaRNA and LocARNA

    PubMed Central

    Smith, Cameron; Heyne, Steffen; Richter, Andreas S.; Will, Sebastian; Backofen, Rolf

    2010-01-01

    The Freiburg RNA tools web server integrates three tools for the advanced analysis of RNA in a common web-based user interface. The tools IntaRNA, ExpaRNA and LocARNA support the prediction of RNA–RNA interaction, exact RNA matching and alignment of RNA, respectively. The Freiburg RNA tools web server and the software packages of the stand-alone tools are freely accessible at http://rna.informatik.uni-freiburg.de. PMID:20444875

  8. End-to-end validation process for the INTA-Nanosat-1B Attitude Control System

    NASA Astrophysics Data System (ADS)

    Polo, Óscar R.; Esteban, Segundo; Cercos, Lorenzo; Parra, Pablo; Angulo, Manuel

    2014-01-01

    This paper describes the end-to-end validation process for the Attitude Control Subsystem (ACS) of the satellite INTA-NanoSat-1B (NS-1B). This satellite was launched on July 2009 and it has been fully operative since then. The development of its ACS modules required an exhaustive integration and a system-level validation program. Some of the tests were centred on the validation of the drivers of sensors and actuators and were carried out over the flying model of the satellite. Others, more complex, constituted end-to-end tests where the concurrency of modules, the real-time control requirements and even the well-formedness of the telemetry data were verified. This work presents an incremental and highly automatised way for performing the ACS validation program based on two development suites and an end-to-end validation environment. The validation environment combines a Flat Satellite (FlatSat) configuration and a real-time emulator working in closed-loop. The FlatSat is built using the NS-1B Qualification Model (QM) hardware and it can run a complete version of the on-board software with the ACS modules fully integrated. The real-time emulator, running on an industrial PC, samples the actuation signals and emulates the sensors signals to close the control loop with the FlatSat. This validation environment constitutes a low-cost alternative to the classical three axes tilt table, with the advantage of being easily configured for working under specific orbit conditions, in accordance with any of the selected tests. The approach has been successfully applied to the NS-1B in order to verify different ACS modes under multiple orbit scenarios, providing an exhaustive coverage and reducing the risk of eventual errors during the satellite's lifetime. The strategy was applied also during the validation of the maintenance and reconfiguration procedures required once the satellite was launched. This paper describes in detail the complete ACS validation process that was performed and it shows the most relevant errors detected and fixed during testing. Finally it summarises some of the most significant conclusions.

  9. Yingna Su CV Astrophysicist

    E-print Network

    Su, Yingna

    Yingna Su ­ CV Astrophysicist Harvard-Smithsonian Center for Astrophysics Education History: B.D. Astrophysics, 2008, Purple Mountain Observatory Predoctoral Fellow, 2005-2007, Harvard-Smithsonian Center for Astrophysics Professional History: 2009/12 - now Astrophysicist, Harvard-Smithsonian Center for Astrophysics

  10. MASTER: CV, DNe, variables.

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Gress, O.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Antipin, S.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Chazov, V.; Vladimirov, V.; Kuvshinov, D.; Shurpakov, S.; Shumkov, V.; Potter, S.; Kniazev, A.; Kotze, M.; Gress, O.; Ivanov, K.; Budnev, N.; Yazev, S.; Poleshchuk, V.; Konstantinov, E.; Chuvalaev, O.; Tlatov, A.; Dormidontov, D.; Senik, V.; Parkhomenko, A.; Yurkov, V.; Sergienko, Y.; Gabovich, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.

    2015-03-01

    MASTER OT J105529.06-255654.5 discovery - new CV MASTER-SAAO auto-detection system ( Lipunov et al., Advances in Astronomy, MASTER Global Robotic Net, 2010 ) discovered OT source at (RA, Dec) = 10h 55m 29.06s -25d 56m 54.5s on 2015-03-11.89942 UT.

  11. CLAMS_CV580_CAR

    Atmospheric Science Data Center

    2014-04-28

    CLAMS_CV580_CAR Project Title:  CLAMS Discipline:  Field ... Radiance in 14 spectral bands Order Data:  ASDC Order Tool:  Order Data Relevant Documents:  CV580 Flight Tracks CAR Home Page Readme Files:  Readme CAR ...

  12. Year Action IB # Jig HAL CP HAL CV Trawl CP Trawl CV

    E-print Network

    Year Action IB # Jig Pot Gear (CP and CV) HAL CP HAL CV Trawl CP Trawl CV TOTAL TAC ABC OFL (CV and CP) HAL CP HAL CV 50 HAL CV CP Trawl CV Rockfish Program Cooperati ves TOTAL Vessel CP = Catcher Processor Rockfish Program Cooperatives this allocation is deducted from the trawl

  13. CV detaille (Detailed CV, in French) Jean-Pierre Nadal

    E-print Network

    Nadal, Jean-Pierre

    CV d´etaill´e (Detailed CV, in French) Jean-Pierre Nadal Directeur de recherche au CNRS Directeur d : nadal@lps.ens.fr Url : http://www.lps.ens.fr/~nadal et Centre d'Analyse et de Math´ematique Sociales UMR@ehess.fr Url : http://cams.ehess.fr/document.php?id=698 1 #12;J.-P. Nadal 2 Contents Curriculum Vitae 3 Annexes

  14. Open CV intro Aside: Histogram Equalization

    E-print Network

    Kosecka, Jana

    Open CV intro Aside: Histogram Equalization Images are from Wikipedia. #12;Today we'll code: A fully functional sparse optical flow algorithm! Plan ·! OpenCV Basics ­! What is it? ­! How do you get started with it? ·! Feature Finding and Optical Flow ­! A brief mathematical discussion. ·! OpenCV

  15. CV

    E-print Network

    2015-10-13

    Oct 13, 2015 ... Algorithms for uncertainty quantification and reduction .... Identification of IKr Kinetics and Drug Binding in Native Myocytes, Annals of Biomedical ..... Grant title: Data Collection & Integration in Cardiac Risk Assessment, 1 R43 ...

  16. CV

    E-print Network

    2015-10-08

    Meetings in Seattle, Washington JMM 2016, January 2016 ... Université du Montréal Summer School on Counting Arithmetic Objects, June 2014 ... Judge for Purdue Undergraduate Research and Poster Symposium Poster session, April 2015 ... Harbridge Consulting Group - actuarial intern Harbridge Consulting Group.

  17. CV

    E-print Network

    2015-12-01

    May 2016. Thesis: Graph diffusions and matrix functions: fast algorithms and ... Research Intern, MIT Lincoln Laboratory, Cyber-anaytics and Decision Systems group ... Summer Research Assistant, Mathematics Department, Fordham University .... Fall 2014 · Instructor · Undergraduate Linear Algebra (MA 264), one section.

  18. CV

    E-print Network

    2015-08-17

    Undergraduate Student Brant Coburn, working on Hecke algebras and quantum spin .... at the Université Paris VI (Pierre et Marie Curie, Jussieu), Paris (France). Studies of ... Pressure measurements at low temperatures, Supervisor: Prof. H. Godfrin ... Presentation of physics experiments to first–graders,. Cumberland ...

  19. mRNA vaccine CV9103 and CV9104 for the treatment of prostate cancer

    PubMed Central

    Rausch, Steffen; Schwentner, Christian; Stenzl, Arnulf; Bedke, Jens

    2014-01-01

    Among currently available vaccine strategies for cancer, nucleotide-based vaccination is an appealing treatment modality. Curevacs’ mRNA containing vaccines (RNActive®) combine the beneficial properties of sufficient antigen-expression, autologous immune-stimulation and a high flexibility with respect to production and application. CV9103 and CV9104 are novel RNActive®-derived anticancer vaccines for the treatment of patients with prostate cancer. After successful phase I/II studies with documentation of good tolerability and favorable immune-activation of CV9103, the vaccine CV9104 is currently undergoing clinical testing in specific clinical settings such as castration resistant prostate cancer and as a neoadjuvant agent in men with high risk prostate cancer prior to surgery. This review discusses the available preclinical and clinical data on the anticancer vaccination treatment with RNActive®-derived anticancer-vaccines CV9103 and CV9104. PMID:25483661

  20. Jared W. Young, CV. CURRICULUM VITAE

    E-print Network

    Squire, Larry R.

    , Department of Psychobiology EDUCATIONAL HISTORY 2001 ­ 2005: Ph.D. in Psychopharmacology, Department-clinical psychopharmacology: British Association for Psychopharmacology 2009: Travel Award: American College of Neuropsychopharmacology #12;Jared W. Young, CV. 2 2008: Outstanding poster presentation in preclinical psychopharmacology

  1. Tutorials on Matlab, OpenCV, and Computer Science Department

    E-print Network

    Jiang, Hao

    Tutorials on Matlab, OpenCV, and SDL Computer Science Department Hao Jiang #12;Matlab · Matlab. · Rewrite bottleneck procedures using c. ­ System(`c_exe'); ­ Compile the function using mex. #12;OpenCV #12;OpenCV · OpenCV is a C library for image processing and computer vision. http

  2. SeeingWithOPenCV FindingFacesin lmages

    E-print Network

    Oh, Paul

    SeeingWithOPenCV FindingFacesin lmages what this mouthfulmeans.Figure1 showsan exampleof OPenCV severalscales. The classifierusesdata storedin an XMLfileto decidehow to classify each image location.The OPenCV andinstallingOpenCV,readingand writingimagefiles,capturingvideo, andworkingwiththelPllmagedata structufe. r

  3. 1/21/14 CV -Deines Research https://sites.google.com/site/deineslab/cv 1/6

    E-print Network

    1/21/14 CV - Deines Research https://sites.google.com/site/deineslab/cv 1/6 Andrew M. Deines and Systematics. 43:-- #12;1/21/14 CV - Deines Research https://sites.google.com/site/deineslab/cv 2/6 2005 Deines, Lodge DM. Influence of fishery effort and #12;1/21/14 CV - Deines Research https://sites.google.com

  4. Robert Gray 1 CV: Robert D. Gray

    E-print Network

    St Andrews, University of

    Robert Gray 1 CV: Robert D. Gray Personal Information Web: http://www.uea.ac.uk/~ fga12juu/ Address: School of Mathematics Telephone: +44 1603 591443 University of East Anglia Norwich NR4 7TJ Email: Robert.D.Gray Scholarship for Training for Algebra Research (Sum- mer 2001). #12;Robert Gray 2 Grants and Fellowships

  5. Elec 331 -Respiratory System Respiratory & CV Systems

    E-print Network

    Pulfrey, David L.

    Elec 331 - Respiratory System 1 Respiratory & CV Systems · Transport ­ Pump · Distribution) during heavy breathing Transport (Respiratory) Natural state of lung #12;Elec 331 - Respiratory System 2: Distribution Exchange Respiratory (Air) #12;Elec 331 - Respiratory System 3 SRC: PNP, p.554 CO2 O2 Cardio

  6. Dipayan (Dip) Biswas: CV (June 2014) DIPAYAN (DIP) BISWAS

    E-print Network

    Jank, Wolfgang

    Dipayan (Dip) Biswas: CV (June 2014) 1 DIPAYAN (DIP) BISWAS University of South Florida BSN 3218 Doctoral Consortium Fellow, Minneapolis, June 2003. #12;Dipayan (Dip) Biswas: CV (June 2014) 2 RESEARCH

  7. The Limbic-Prefrontal Network Modulated by Electroacupuncture at CV4 and CV12

    PubMed Central

    Fang, Jiliang; Wang, Xiaoling; Liu, Hesheng; Wang, Yin; Zhou, Kehua; Hong, Yang; Liu, Jun; Wang, Lei; Xue, Chao; Song, Ming; Liu, Baoyan; Zhu, Bing

    2012-01-01

    fMRI studies showed that acupuncture could induce hemodynamic changes in brain networks. Many of these studies focused on whether specific acupoints could activate specific brain regions and were often limited to manual acupuncture at acupoints on the limbs. In this fMRI study, we investigated acupuncture's modulation effects on brain functional networks by electroacupuncture (EA) at acupoints on the midline of abdomen. Acupoints Guanyuan (CV4) and Zhongwan (CV12) were stimulated in 21 healthy volunteers. The needling sensations, brain activation, and functional connectivity were studied. We found that the limbic-prefrontal functional network was deactivated by EA at CV4 and CV12. More importantly, the local functional connectivity was significantly changed during EA stimulation, and the change persisted during the period after the stimulation. Although minor differences existed, both acupoints similarly modulated the limbic-prefrontal functional network, which is overlapped with the functional circuits associated with emotional and cognitive regulation. PMID:22291848

  8. Ms informacon: http://cv1.cpd.ua.es/ConsPlanesEstudio/cvFi-

    E-print Network

    Escolano, Francisco

    Más informacíon: http://cv1.cpd.ua.es/ConsPlanesEstudio/cvFi- chaAsiEEES.asp?wCodEst=C053&wcodasi=2 6048&wLengua=C&scaca=2012-13 Departamento de Química Física Correo electrónico: dqfis@ua.es Teléfono: 965903536 Página web: http://dqf.ua.es/es/ Diseño:GabinetedeImagenyComunicaciónGráficadelaUniversidaddeAlicante.Impressió:imprenta.ua

  9. SeeingWithOpenCV FollowThatFace!

    E-print Network

    Oh, Paul

    SeeingWithOpenCV FollowThatFace! describedin lastmonth'sarticle.To do that, however,you'd needto'dnepdto handlethatJ v t v u situation,aswell. Fortunately,OpenCV includes specializedcode for trackinga faceCVtotrackataceonce you'vedetectedit. FaceTrackingin OpenCV Trackinga face is more drfficult thantrackinga strongly

  10. Earth, Moon, Sun, and CV Accretion Disks

    E-print Network

    Montgomery, M M

    2009-01-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting Cataclysmic Variable (CV) Dwarf Novae systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar and black hole systems. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our a...

  11. Earth, Moon, Sun, and CV Accretion Disks

    NASA Astrophysics Data System (ADS)

    Montgomery, M. M.

    2009-11-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if accretion disks are present or not. Our results suggest that the accretion disk's geometric shape directly affects the disk's precession rate.

  12. EARTH, MOON, SUN, AND CV ACCRETION DISKS

    SciTech Connect

    Montgomery, M. M.

    2009-11-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if accretion disks are present or not. Our results suggest that the accretion disk's geometric shape directly affects the disk's precession rate.

  13. Cloning and sequencing of Duck circovirus (DuCV).

    PubMed

    Hattermann, K; Schmitt, C; Soike, D; Mankertz, A

    2003-12-01

    The genome of Duck circovirus (DuCV) is circular and 1996 nts in size. Two major open reading frames were identified, encoding the replicase (V1) and the capsid protein (C1). A stem-loop structure comprising the nonamer 5'-TATTATTAC, conserved in all circo-, nano- and geminiviruses, was found. Unique to DuCV, the region between the 3'-ends of the rep and cap gene contains four repeats of a 44-bp sequence. Phylogenetic analysis shows close relation of DuCV with Goose circovirus and suggests classification of DuCV as a new member of the genus Circovirus of the virus family Circoviridae. PMID:14648300

  14. MIZEX, 1984, NASA CV-990 flight report

    NASA Technical Reports Server (NTRS)

    1985-01-01

    During June/July 1984, the NASA CV-990 Airborne Laboratory was utilized in a mission to overly the Fram Strait/East Greenland Sea marginal ice zone (MIZ) during the main summer marginal ice zone experiment (MIZEX '84). The eight data flights were coordinated where possible with overpasses of the Nimbus-7 satellite, and with measurement of sea ice, open ocean, and atmospheric properties at the surface. The surface research teams were based on seven research vessels, some with helicopters: (1) M/V Kvitbjorn, (2) M/V Polarqueen; (3) M/S Haakon Mosby; (4) a M/S H.U. Sverdrup, all from Norway; (5) F/S Polarstern from the Federal Republic of Germany; and (6) the USNS Lynch from the USA. There were also coordinated flights with the NRL P3, NOAA P3, Canadian CV580, and the French B-17 during the overlap portions of their respective missions. Analysis of the real-time data acquired during the mission and uncalibrated data stored on tape has served to indicate the mission was over 90% successful.

  15. James F. Gilchrist cv -page 1/16

    E-print Network

    Gilchrist, James F.

    James F. Gilchrist cv - page 1/16 James F. Gilchrist ­ Curriculum Vitae A. Biographical Information at Harvard University) #12;James F. Gilchrist cv - page 2/16 B. Publications and Creative Activities Refereed Articles 32) T. Muangnapoh, A. L. Weldon, and J. F. Gilchrist, "Enhanced monolayer deposition via vibration

  16. C.V. Yanli Zhang Page 1 of 10

    E-print Network

    Zhang, Yanli

    C.V. Yanli Zhang Page 1 of 10 Yanli Zhang, Ph.D. Assistant professor of Spatial Science and Water in 21st century China #12;C.V. Yanli Zhang Page 2 of 10 PUBLICATIONS Peer Reviewed Articles 1. Zhang, Y., D. Unger, Y. Zhang, P. Bedford, and I. Hung. 2014. Comparing remotely sensed Pictometry web based

  17. NASA/ESA CV-990 spacelab simulation

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Due to interest in the application of simplified techniques used to conduct airborne science missions at NASA's Ames Research Center, a joint NASA/ESA endeavor was established to conduct an extensive Spacelab simulation using the NASA CV-990 airborne laboratory. The scientific payload was selected to perform studies in upper atmospheric physics and infrared astronomy with principal investigators from France, the Netherlands, England, and several groups from the United States. Communication links between the 'Spacelab' and a ground based mission operations center were limited consistent with Spacelab plans. The mission was successful and provided extensive data relevant to Spacelab objectives on overall management of a complex international payload; experiment preparation, testing, and integration; training for proxy operation in space; data handling; multiexperimenter use of common experimenter facilities (telescopes); multiexperiment operation by experiment operators; selection criteria for Spacelab experiment operators; and schedule requirements to prepare for such a Spacelab mission.

  18. Pitfalls in the detection of CV2 (CRMP5) antibodies.

    PubMed

    Sabater, Lidia; Saiz, Albert; Dalmau, Josep; Graus, Francesc

    2016-01-15

    CV2 antibodies (CV2-ab) associate with paraneoplastic neurological syndromes (PNS) and small-cell lung cancer. This study was designed to assess the sensitivity of two widely used anti-CV2 commercial kits. Fifty three sera with CV2-ab identified by immunohistochemistry on paraformaldehyde-perfused rat brain were tested with two commercial immunoblot kits (Euroimmun AG, and Ravo Diagnostika) and 4 (7.5%) of them were negative with the commercial kits. The 4 samples were positive by immunofluorescence on HEK293 cells transfected with CRMP5 and immunoblot of these cells lysate. A few CV2-ab-positive sera may be missed by commercial immunoblots. Negative samples from patients with high suspicion for PNS should be tested by alternative methods. PMID:26711575

  19. Ch OpenCV for interactive open architecture computer vision Qingcang Yua

    E-print Network

    Cheng, Harry H.

    Ch OpenCV for interactive open architecture computer vision Qingcang Yua , Harry H. Chenga,*, Wayne and implementation of an interactive open architecture computer vision software package called Ch OpenCV is presented. Benefiting from both Ch and OpenCV, Ch OpenCV has many salient features. It is interactive, capable

  20. Opaque Assemblages in CK and CV Carbonaceous Chondrites

    NASA Technical Reports Server (NTRS)

    Neff, K. E.; Righter, K.

    2006-01-01

    CK carbonaceous chondrites are the only group of carbonaceous chondrites that exhibit thermal metamorphism. As a result, CKs display features of metamorphism such as silicate darkening, recrystallization and shock veins. Calcium Aluminum Inclusions and Fe-Ni metal are rare. CV carbonaceous chondrites are unequilibrated and have two subgroups; oxidized and reduced. The CV and CK carbonaceous chondrite groups have been compared to each other often because of petrographic similarities, such as overlapping oxygen isotopic ratios. Scientists have suggested the two groups of carbonaceous chondrites formed from the same parent body and CKs are equilibrated CV chondrites [1, 2]. The oxidized CV group has been most closely related to CKs. This study examines the petrology and mineralogy of CKs and CVs focusing on opaque minerals found in the meteorites. Using the oxide, metal and sulfide assemblages, constraints can be placed on the temperature and oxygen fugacity at which the meteorites equilibrated. The temperature and oxygen fugacity of the CK and CV chondrites can be compared in order to help define their formation history.

  1. Taylor, M.H. CV 1 MICHAEL H. TAYLOR

    E-print Network

    , Llanos basin, Colombia, submitted, Tectonics 5. Silver C., M. Murphy, M. Taylor, J, Gosse, T. 2015, Baltz, 33, doi:10.1002/2013TC003302. 4. M. Murphy, M. Taylor, J. Gosse, C. Silver, D. Whip, C. BeaumontTaylor, M.H. CV 1 MICHAEL H. TAYLOR The University of Kansas Department of Geology 114 Lindley Hall

  2. CV: Leigh G. Torres Leigh G. Torres, Ph.D.

    E-print Network

    Kurapov, Alexander

    CV: Leigh G. Torres Leigh G. Torres, Ph.D. Assistant Professor, Department of Fisheries, climate change and habitat alterations. Publications a) Peer-reviewed journal publications Torres, L.G., P through spatial analysis and genetics. Marine Mammal Science 19(3):502-514. Torres, L.G., W.A. McLellan, E

  3. K. D. Bockrath CV 1 Katherine D. Bockrath

    E-print Network

    Arnold, Jonathan

    , Matthew J. Hill. (2013). The Mussel-Fish Relationship: A Potential New Twist in North American Unionidae? Transactions of the American Fisheries Society 142 (3), 642-648 #12;K. D. Bockrath CV 2 Posters and Presentations Invited Talks 9/2014 How Freshwater Mussel Glochidia use Alabama Shad in the ACF. Alabama Shad

  4. R. MICHAEL YOUNG CV JANUARY 2015 R. Michael Young

    E-print Network

    Young, R. Michael

    organizations at the national, state and local levels. Young's research looks primarily at new models of plans!1 R. MICHAEL YOUNG CV JANUARY 2015 R. Michael Young Professor Department of Computer Science Campus Box 8206 North Carolina State University Raleigh, NC, 27513-8206 919.513.3038 young

  5. CV Renana Ofan page 1 of 3 Renana H. Ofan

    E-print Network

    CV Renana Ofan page 1 of 3 Renana H. Ofan New York University Phone: 212-998-7780 Center for Neural Science Cell: 646-660-3481 4 Washington Pl. room 809 Fax: 212-994-4011 New York, NY, 10003 email: ofan: Professor David Heeger Investigated cortical mapping of spatial attention in the parietal cortex using f

  6. Computational simulation of CV combination preferences in babbling.

    PubMed

    Nam, Hosung; Goldstein, Louis M; Giulivi, Sara; Levitt, Andrea G; Whalen, D H

    2013-03-01

    There is a tendency for spoken consonant-vowel (CV) syllables, in babbling in particular, to show preferred combinations: labial consonants with central vowels, alveolars with front, and velars with back. This pattern was first described by MacNeilage and Davis, who found the evidence compatible with their "frame-then-content" (F/C) model. F/C postulates that CV syllables in babbling are produced with no control of the tongue (and therefore effectively random tongue positions) but systematic oscillation of the jaw. Articulatory Phonology (AP; Browman & Goldstein) predicts that CV preferences will depend on the degree of synergy of tongue movements for the C and V. We present computational modeling of both accounts using articulatory synthesis. Simulations found better correlations between patterns in babbling and the AP account than with the F/C model. These results indicate that the underlying assumptions of the F/C model are not supported and that the AP account provides a better and account with broader coverage by showing that articulatory synergies influence all CV syllables, not just the most common ones. PMID:24496111

  7. An Energy-Efficient, Fast FPGA Hardware Architecture for OpenCV-Compatible Object

    E-print Network

    Rose, Jonathan

    An Energy-Efficient, Fast FPGA Hardware Architecture for OpenCV-Compatible Object Detection Braiden source OpenCV computer vision software. The architecture is novel, compared to prior work in this area Computer Vision project, OpenCV [3]. We should note that the mobile context of this paper rests

  8. An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection

    E-print Network

    Rose, Jonathan

    An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection for the Acceleration of OpenCV Object Detection Braiden Brousseau Master of Applied Science Graduate Department . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.4 OpenCV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.5 Multi

  9. An Intensive HST/COS Study of 31 CV WDs

    NASA Astrophysics Data System (ADS)

    Pala, A. F.; Gänsicke, B. T.; Beuermann, K.; Bildsten, L.; De Martino, D.; Godon, P.; Henden, A. A.; Hubeny, I.; Knigge, C.; Long, K. S.; Marsh, T. R.; Patterson, J.; Schreiber, M. R.; Sion, E. M.; Szkody, P.; Townsley, D.; Zorotovic, M.

    2015-06-01

    Accretion is the key ingredient in the evolution of white dwarfs (WDs) in cataclysmic variables (CVs), with important implications for their potential as progenitors for Type Ia Supernovae. The effective temperature (Teff) gives a direct measure of the average accretion rate and we report here the Teff measurement for 31 CV WDs, observed as a part of a 122 orbit HST program. The HST spectra alone cannot resolve the degeneracy between Teff and the surface gravity log g, and we will use phase-resolved X-Shooter observations to (i) measure the mass ratio of the CVs from the reflex motion of both the WD and the donor star (ii) to establish the spectral energy distribution (SED), which constrains both Teff and log g. Combining the X-Shooter and HST data, we can measure the WD masses to a few percent, and will be able to answer the question whether accreting CV WDs grow in mass.

  10. Axtrell, a new CV3 chondrite find from Texas

    NASA Technical Reports Server (NTRS)

    Simon, S. B.; Grossman, L.; Casanova, I.; Symes, S.; Benoit, P.; Sears, D. W. G.; Wacker, J. F.

    1995-01-01

    We describe a previously unreported meteorite found in Axtell, Texas, in 1943. Based on the mineralogical composition and texture of its matrix and the sizes and abundance of chondrules, we classify it as a CV3 carbonaceous chondrite. The dominant opaque phase in the chondrules is magnetite, and that in refractory inclusions is Ni-rich metal (awaruite). Axtell, therefore, belongs to the oxidized subgroup of CV3 chondrites, although unlike Allende it escaped strong sulfidation. The meteorite bears a strong textural resemblance to Allende, and its chondrule population and matrix appear to be quite similar to those of Allende, but its refractory inclusions, thermoluminescence properties, and cosmogenic Co-60 abundances are not. Our data are consistent with a terrestrial age for Axtell of approximately 100 years and a metamorphic grade slightly lower than that of Allende.

  11. Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China.

    PubMed

    Zhang, Zhicheng; Dai, Wei; Wang, Shaohui; Dai, Dingzhen

    2015-01-01

    Pigeon circovirus (PiCV) is one of four viruses in the family Circoviridae that affect young pigeons around the world. We collected 158 serum or tissue samples from six poultry farms in eastern China to investigate the prevalence and genetic characteristics of PiCV in Chinese pigeons. We tested for PiCV using a PCR assay and found that PiCV was present in 80.7 % (88/109) of diseased pigeons and 63.3 % (31/49) of healthy pigeons; overall, 75.3 % (119/158) of samples were PiCV positive. One PiCV-positive sample from each poultry farm was randomly chosen for amplification of the complete PiCV genome by inverse primer PCR (IP-PCR). The six genomic PiCV strains were designated as AHBZ (KJ704801), HBLF-E2 (KJ704802), JSJN (KJ704803), NJPK-21 (KJ704804), SDDZ (KJ704805) and SHWH-AB4 (KJ704806). We compared these new PiCV genomes to six publicly available PiCV genomes and found that the Rep and Cap genes had sequence identity ranging from 93.8 % to 100 % and 79.1 % to 100 %, respectively. In a phylogenetic analysis, PiCV and eight other members of the genus Circovirus were sister to chicken anemia virus (CAV), the only member of genus Gyrovirus. The results of this study provide evidence that PiCV is present in Chinese pigeons at a high rate and that PiCV is a viral lineage that is distinct from CAV. PMID:25348272

  12. The All-Sky Automated Survey for Supernovae CV Patrol

    NASA Astrophysics Data System (ADS)

    Davis, Alexandra Bianca; Shappee, Benjamin John; Archer Shappee, Bartlett; ASAS-SN

    2015-01-01

    Even in the modern era, only human eyes scan the entire optical sky for the violent, variable, and transient events that shape our universe. The "All Sky Automated Survey for Supernovae" (ASAS-SN or "Assassin") is changing this by monitoring the extra-galactic sky down to V~17 mag every 2-3 days using multiple telescopes, hosted by Las Cumbres Observatory Global Telescope Network, in the northern and southern hemispheres. By far the most common events observed by ASAS-SN are the Galactic transients. Since April 2013 ASAS-SN has identified over 180 new cataclysmic variable stars and announced over 260 new outbursts of known CVs. To make our data available to the CV community in 'real time', we have launched an automated 'CV Patrol' to monitor known CVs for outbursts as a useful tool for both professional and amateurs astronomers. It is a long term goal of ASAS-SN to make all our data public in real-time, and this patrol will serve as a framework for future ASAS-SN data releases.

  13. Some physical, pomological and nutritional properties of kiwifruit cv. Hayward.

    PubMed

    Celik, Ahmet; Ercisli, Sezai; Turgut, Nihat

    2007-09-01

    In this research, several physical, pomological and nutritional properties that are important for the design of equipments for harvesting, processing, transportation, sorting, separation and packaging of kiwifruit cv. Hayward grown in the Black Sea region of Turkey were determined. The fruit characteristics ranged from 72.28 g for average fruit weight, 59.41, 46.28 and 42.87 mm for fruit length, width and thickness, 49.03 mm for the geometric mean diameter, 0.825% for sphericity and 66.52 cm(3) for the volume of fruit, respectively. The bulk density, fruit density and porosity were determined as 575.27 kg/m(3), 1,093 kg/m(3) and 47.13%. The highest coefficient of static friction was obtained on plywood as 0.190, followed by polyethylene, rubber and galvanized steel sheet as 0.173, 0.163 and 0.158, respectively. The total soluble solid content, acidity, vitamin C, ash and total nitrogen content of kiwifruit cv. Hayward were 7.32%, 1.64%, 108 mg/100g, 0.71 g/100 g and 0.84%, respectively. The fresh fruits have 1.09 mg/100g total chlorophylls and flesh color data represented as L, a and b were 57.18, 17.25 and 37.46, respectively. PMID:17710584

  14. No nebular magnetization in the Allende CV carbonaceous chondrite

    NASA Astrophysics Data System (ADS)

    Fu, R. R.; Lima, E. A.; Weiss, B. P.

    2014-10-01

    Magnetic fields in the solar nebula may have played a central role in mass and angular momentum transport in the protosolar disk and facilitated the accretion of the first planetesimals. Thought to be key evidence for this hypothesis is the high unblocking-temperature, randomly oriented magnetization in chondrules in the Allende CV carbonaceous chondrite. However, it has recently been realized that most of the ferromagnetic minerals in Allende are products of secondary processes on the parent planetesimal. Here we reevaluate the pre-accretional magnetism hypothesis for Allende using new paleomagnetic analyses of chondrules including the first measurements of mutually oriented subsamples from within individual chondrules. We confirm that Allende chondrules carry a high-temperature component of magnetization that is randomly oriented among chondrules. However, we find that subsamples of individual chondrules are also non-unidirectionally magnetized. Therefore, the high-temperature magnetization in Allende chondrules is not a record of nebular magnetic fields and is instead best explained by remagnetization during metasomatism in a <8 ?T magnetic field. This low field intensity suggests that any core dynamo on the CV parent body decayed before the end of metasomatism, likely <40 My after the formation of calcium aluminum-rich inclusions (CAIs). Despite widespread practice, the magnetization in Allende should not be used to constrain magnetic fields in the protosolar nebula.

  15. Collisional facilitation of aqueous alteration of CM and CV carbonaceous chondrites

    NASA Astrophysics Data System (ADS)

    Rubin, Alan E.

    2012-08-01

    CM chondrites exhibit a strong correlation between the degree of alteration and the extent of particle alignment (i.e., the strength of the petrofabric). It seems likely that the S1 shock stage of essentially every CM and the high matrix abundance (˜70 vol.%) of these samples ensured that the shock waves that produced CM petrofabrics (by collapsing matrix pores and squeezing chondrules into pore spaces) were significantly attenuated and were too weak to damage olivine crystal lattices. Random collisions on the CM body produced petrofabrics and created fractures in the target rocks. Subsequent impact-mobilization of water caused hydrated phases to form preferentially in the more-fractured regions (those with the strongest petrofabrics); the less-deformed, less-fractured CM regions experienced lower degrees of aqueous alteration. Many CV3 chondrites also have petrofabrics: roughly half are from the oxidized Bali-like subgroup (CV3OxB), roughly half are from the reduced subgroup (CV3R) and none is from the oxidized Allende-like subgroup (CV3OxA) (which is less altered than CV3OxB). Nearly all CVs with petrofabrics are S3-S4 and nearly all CVs that lack petrofabrics are S1. Oxidized CVs have much higher porosities (typically 20-28%) than reduced CVs (0.6-8%), facilitating more-extensive aqueous alteration. The CV3R chondrites formed from low-porosity material that inhibited oxidation during alteration. The oxidized CV subgroups formed from higher-porosity materials. The CV3OxB samples were shocked, became extensively fractured and developed petrofabrics; the CV3OxA samples were not shocked and never developed petrofabrics. When water was mobilized, both sets of porous CV chondrites became oxidized; the more-fractured CV3OxB subgroup was more severely altered.

  16. Prof. C. Patrick Yue CV Chik Patrick Yue, Ph.D.

    E-print Network

    Yue, Patrick

    Prof. C. Patrick Yue ­ CV Chik Patrick Yue, Ph.D. Mobile: +852 9789-5981 | eepatrick@ust.hk | http-time), EE Department 2001 ­ 2003 #12;Prof. C. Patrick Yue ­ CV Industry Experience Non-executive Director

  17. Solution NMR Structure of Hypothetical Protein CV_2116 Encoded by a Viral Prophage Element in Chromobacterium violaceum

    SciTech Connect

    Yang, Yunhuang; Ramelot, Theresa A.; Cort, John R.; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl H.; Kennedy, Michael A.

    2012-06-14

    CV{_}2116 from Chromobacterium violaceum is a small hypothetical protein of 82 amino acids. A PSI-BLAST search using the CV{_}2116 protein sequence as a query identified only two hits, both with amino acid sequence identities of less than 40%. After the CV{_}2116 gene was cloned into the p15TvLic expression plasmid and transformed into E. coli, the desired CV{_}2116 protein was expressed and purified. A high quality solution structure of CV{_}2116 was determined by NMR spectroscopy. The tertiary structure of CV{_}2116 adopts a novel alpha + beta fold containing two anti-parallel beta sheets and one alpha helix in the C-terminal end. CV{_}2116 does not belong to any known protein sequence families and no similar structures exist in the protein data bank. To date, no function of CV{_}2116 can be derived from either sequence or structural similarity searches.

  18. Ryegrass cv. Lema and guava cv. Paluma biomonitoring suitability for estimating nutritional contamination risks under seasonal climate in Southeastern Brazil.

    PubMed

    Bulbovas, Patricia; Camargo, Carla Z S; Domingos, Marisa

    2015-08-01

    The risks posed by nutrient deposition due to air pollution on ecosystems and their respective services to human beings can be appropriately estimated by bioindicator plants when they are well acclimated to the study region environmental conditions. This assumption encouraged us to comparatively evaluate the accumulation potential of ryegrass cv. Lema and guava cv. Paluma macro and micronutrients. We also indicated the most appropriate species for biomonitoring nutrient contamination risks in tropical areas of Southeastern Brazil, which are characterized by marked dry and wet seasons and complex mixtures of air pollutants from different sources (industries, vehicle traffic and agriculture). The study was conducted in 14 sites with different neighboring land uses, within the Metropolitan Region of Campinas, central-eastern region of São Paulo State. The exposure experiments with ryegrass and guava were consecutively repeated 40 (28 days each) and 12 (84 days each) times, respectively, from Oct/2010 to Sept/2013. Macro and micronutrients were analyzed and background concentrations and enrichment ratios (ER) were estimated to classify the contamination risk within the study region. Significantly higher ER suggested that ryegrass were the most appropriate accumulator species for N, S, Mg, Fe, Mn, Cu and Zn deposition and guava for K, Ca, P and B deposition. Based on these biomonitoring adjustments, we concluded that the nutrient deposition was spatially homogeneous in the study area, but clear seasonality in the contamination risk by nutritional inputs was evidenced. Significantly higher contamination risk by S, Fe, K and B occurred during the dry season and enhanced contamination risk by Mn, Cu and Zn were highlighted during the wet season. Distinctly high contamination risk was estimated for S, Fe and Mn in several exposure experiments. PMID:25938695

  19. Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual Machine

    E-print Network

    California at Santa Barbara, University of

    Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual jblynch@ucsd.edu Abstract The OpenCV framework is an open source project released under the BSD license will focus on the OpenCV framework, an open source project that furnishes developers with a series

  20. Processing in a transitional environment of CV and CK chondrites' parent bodies in the light of mineralogical and petrological analysis of NWA 1465 CV3 meteorite

    NASA Astrophysics Data System (ADS)

    Kereszturi, A.; Ormandi, Sz.; Jozsa, S.

    2015-05-01

    CV and CK chondrites might came from the same parent body from different depth values. To better constrain the conditions there, the composition, structure and chronology of mineral changes were analyzed in NWA 1465 CV3 chondrite meteorite. This sample represents an interesting part of the proposed ancient parent body where opposite to the generally poor altered state of CV3 chondrites, strong mechanical deformation produced elongated chondrules. All of the ingredients in the sample were flattened and fractured. After the chondrules settled down glass formation, opaque crystallization, fracturing and a second opaque generation followed by carbonate crystallization happened. The lack of fragments along the veins suggests no shearing movement happened, and the small number of weak shock signatures points to S2 level, and the oriented pressure in the parent body might come from burial induced pressure. The meteorite might came from a location in the CV-CK parent body where melting, and weak impact related fracturing and vein filling happened. In this an environment alteration of the primitive material started, possibly from a zone transitional between the shallow, less processed CV, and the deeper, more processed CK chondrites.

  1. Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) Somaclones

    NASA Technical Reports Server (NTRS)

    Campbell, W. F.; Carman, J. G.; Hashim, Z. N.

    1990-01-01

    The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.

  2. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  3. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-05-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  4. Seven health physics calculator programs for the HP-41CV

    SciTech Connect

    Rittmann, P.D.

    1984-08-01

    Several user-oriented programs for the Hewlett-Packard HP-41CV are explained. The first program builds, stores, alters, and ages a list of radionuclides. This program only handles single- and double-decay chains. The second program performs convenient conversions for the six nuclides of concern in plutonium handling. The conversions are between mass, activity, and weight percents of the isotopes. The source can be aged and/or neutron generation rates can be computed. The third program is a timekeeping program that improves the process of manually estimating and tracking personnel exposure during high dose rate tasks by replacing the pencil, paper, and stopwatch method. This program requires a time module. The remaining four programs deal with computations of time-integrated air concentrations at various distances from an airborne release. Building wake effects, source depletion by ground deposition, and sector averaging can all be included in the final printout of the X/Q - Hanford and X/Q - Pasquill programs. The shorter versions of these, H/Q and P/Q, compute centerline or sector-averaged values and include a subroutine to facilitate dose estimation by entering dose factors and quantities released. The horizontal and vertical dispersion parameters in the Pasquill-Gifford programs were modeled with simple, two-parameter functions that agreed very well with the usual textbook graphs. 8 references, 7 appendices.

  5. Limited geographic distribution of the novel cyclovirus CyCV-VN

    PubMed Central

    Tan, Le Van; de Jong, Menno D.; Van Kinh, Nguyen; Trung, Nguyen Vu; Taylor, Walter; Wertheim, Heiman F. L.; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Uyen, Nguyen Hanh; Giri, Abhishek; Thi Thuy Chinh BKrong, Nguyen; Nghia, Ho Dang Trung; Farrar, Jeremy; Bryant, Juliet E.; Hien, Tran Tinh; Van Vinh Chau, Nguyen; van Doorn, H. Rogier

    2014-01-01

    A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600?CSF specimens from patients with suspected CNS infections in northern Vietnam, Cambodia, Nepal and The Netherlands were screened for the presence of CyCV-VN but all were negative. Sequence comparison and phylogenetic analysis between CyCV-VN and another novel cyclovirus recently identified in CSF from Malawian patients indicated that these represent distinct cycloviral species, albeit phylogenetically closely related. The data suggest that CyCV-VN has a limited geographic distribution within southern and central Vietnam. Further research is needed to determine the global distribution and diversity of cycloviruses and importantly their possible association with human disease. PMID:24495921

  6. THE BANANA PROJECT. V. MISALIGNED AND PRECESSING STELLAR ROTATION AXES IN CV VELORUM

    E-print Network

    Albrecht, Simon H.

    As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find ...

  7. Broadcast with Heterogeneous Node Capability Intae Kang and Radha Poovendran

    E-print Network

    Poovendran, Radha

    data-gathering stations which can also serve as gateway nodes to infrastructure networks. Also to disseminate GPS or location information throughout the sensor nodes. Be it a gateway, an access point of the U.S government. possess homogeneous capability and hence bidirectional links are assumed

  8. Detoxification of microcystin-LR in water by Portulaca oleracea cv.

    PubMed

    Isobe, Takatoshi; Okuhata, Hiroshi; Miyasaka, Hitoshi; Jeon, Bong-Seok; Park, Ho-Dong

    2014-03-01

    Microcystin-LR (0.02 ?g/ml) in the hydroculture medium of Portulaca oleracea cv., became below the detection level (<0.0001 ?g/ml) by HPLC analysis after 7 days. The toxicity of microcystin estimated with protein phosphatase inhibition assay, however, remained at 37% of the initial level, indicating that microcystin-LR was transformed by P. oleracea cv. into unknown compound(s) of lower toxicity. PMID:23999063

  9. Defense response of a pepper cultivar cv. Sy-2 is induced at temperatures below 24°C.

    PubMed

    Koeda, Sota; Hosokawa, Munetaka; Kang, Byoung-Cheorl; Tanaka, Chihiro; Choi, Doil; Sano, Satoshi; Shiina, Takashi; Doi, Motoaki; Yazawa, Susumu

    2012-01-01

    Temperature is one of the most important environmental factors that influence plant growth and development. Recent studies imply that plants show various responses to non-extreme ambient temperatures. Previously, we have found that a pepper cultivar cv. Sy-2 (Capsicum chinense) shows developmental defects at temperatures below 24°C. In this study, to gain new insights into the temperature sensitivity of cv. Sy-2, temperature-sensitive genes were screened using microarray techniques. At restrictive temperature of 20°C, almost one-fourth of the 411 up-regulated genes were defense related or predicted to be defense related. Further expression analyses of several defense-related genes showed that defense-related genes in cv. Sy-2 were constitutively expressed at temperatures below 24°C. Moreover, accumulation of high level of salicylic acid (SA) in cv. Sy-2 grown at 20°C suggests that the defense response is activated in the absence of pathogens. To confirm that the defense response is induced in cv. Sy-2 below 24°C, we evaluated the resistance to biotrophic bacterial pathogen Xanthomonas campestris pv. vesicatoria and necrotrophic fungal pathogen Cercospora capsici. Cv. Sy-2 showed enhanced resistance to X. campestris pv. vesicatoria, but not to C. capsici. PMID:21424609

  10. Figure 3: Overexpression of AtCV led to chloroplast degradation Figure 5: AtCV interacts with photosystem II subunit PsbO1 in vivo

    E-print Network

    Blumwald, Eduardo

    stresses affect crop yields by limiting plant growth and accelerating leaf senescence. The earliest mediates plant chloroplast stability during the response of plants to abiotic stresses. E : Envelope and oxidative stress Mock NaCl MV Relativeexpression(2CT) ProAtCV::GUS Young Old Figure 4: Silencing At

  11. Visualization of HDF/HDF-EOS Format Earth Observing System Data Using the ISIS "cv" Program

    NASA Astrophysics Data System (ADS)

    Torson, J. M.

    2001-05-01

    The "cv" (Cube Visualization) program has been used for a number of years as part of the ISIS image processing system (Integrated Software for Imagers and Spectrometers) [1],[2]. In addition to reading the native ISIS image cube format, "cv" has recently been enhanced to directly read the HDF and HDF-EOS file formats used for distributing data from various Earth Observing System (EOS) Missions (e.g. the Terra and Landsat-7 satellites). Files based on HDF Version 4.x are supported; files based on the new HDF 5.x are not yet supported. "cv" is now packaged so that it can be used without installing the rest of the ISIS software system. The capabilities of the program include: Displays (as images) any Swath/Grid data fields in HDF-EOS files; Displays (as images) any Scientific Data Set (SDS) data fields in HDF files; Combines multiple HDF/HDF-EOS fields to form one display object; Subarea selection and/or subsampling (allows handling large files); Simultaneous display of multiple images/files; Plots intensity profiles along any of the three axes in a 3D data set; Writes displayed data fields to binary files (allows doing further processing using ISIS programs or using other software packages); Reports cursor location and pixel value (includes reporting Latitude/Longitude with optional conversion between geocentric and geographic coordinates); Includes many additional flexible display options. The "cv" program is implemented in the IDL language and makes use of the IDL CALL_EXTERNAL capability to call I/O and utility routines written in C and Fortran. Pre-compiled versions of "cv" are available for Sun Solaris, Compaq Alpha and PC Linux platforms. To obtain "cv", go to the USGS anonymous ftp site (ftpflag.wr.usgs.gov). Do a "cd dist/isis" and get (in binary mode) the README_CV.TXT file (installation instructions) and the tar file for the desired platform (cv_sun.tar, cv_alpha.tar, cv_pc.tar). More information on ISIS is available at the ISIS website (http://wwwflag.wr.usgs.gov/ISIS). References: [1] Torson, J.M., and Becker, K.J., (1997), ISIS - A Software Architecture for Processing Planetary Images, Lunar and Plan. Sci. XXVIII, 387-388, [2] Gaddis, L., (1996), Use of ISIS for Processing and Analysis of Planetary Remote Sensing data, GSA Abstracts with Programs, v. 28, No. 7, p. A-286.

  12. Mineralogy and Petrography of MIL 090001, a Highly Altered CV Chondrite from the Reduced Sub-Group

    NASA Technical Reports Server (NTRS)

    Keller, Lindsay P.

    2011-01-01

    MIL 090001 is a large (greater than 6 kg) CV chondrite from the reduced subgroup (CV(sub red)) that was recovered during the 2009-2010 ANSMET field season [1]. The CV(sub red) subgroup meteorites retain primitive characteristics and have escaped the Na and Fe meta-somatism that affected the oxidized (CV(sub ox)) subgroups. MIL 090001 is, however, reported to be altered [1], and thus a major objective of this study is to characterize its mineralogy and petrography and the extent of the alteration.

  13. Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.

    PubMed

    Matsushima, Kazuki; Kaneda, Hirokazu; Harada, Kazuo; Matsuura, Hideyuki; Hirata, Kazumasa

    2015-05-01

    Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater. PMID:25700813

  14. Analysis of Positive Superhump Shapes Near Superoutburst Maximum in CV SU UMa-like Systems

    NASA Astrophysics Data System (ADS)

    Bobertz, Michele; Voloshina, Irina; Goel, Amit

    2016-01-01

    Positive superhumps are hump-shaped modulations in light curves of some Cataclysmic Variables (CVs) that have a period that is slightly longer than the orbital period. In CV SU UMa-like systems, the shape of the positive superhump is known to change throughout the superoutburst, which thus slightly changes the published, observed, positive superhump period. In this presentation, we analyze numerical simulations of prograde precession in accretion disks of CV SU UMa-like systems near superoutburst maximum. We compare the simulated positive superhump shapes with the shapes obtained from observations, using AW Sge as our model. Similarly, we compare associated Fourier Transforms with associated periodograms. We conclude with our analysis of the likely sources that generate the shape of the positive superhump in CV SU UMa-like systems near superoutburst maximum.

  15. Segmentation of kidney using C-V model and anatomy priors

    NASA Astrophysics Data System (ADS)

    Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

    2007-12-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

  16. CV-Muzar - The Virtual Community Environment that Uses Multiagent Systems for Formation of Groups

    NASA Astrophysics Data System (ADS)

    de Marchi, Ana Carolina Bertoletti; Moraes, Márcia Cristina

    The purpose of this chapter is to present two agents' societies responsible for group formation (sub-communities) in CV-Muzar (Augusto Ruschi Zoobotanical Museum Virtual Community of the University of Passo Fundo). These societies are integrated to execute a data mining classification process. The first society is a static society that intends preprocessing data, investigating the information about groups in the CV-Muzar. The second society is a dynamical society that will make a classification process by analyzing the existing groups and look for participants that have common subjects in order to constitute a sub-community. The formation of sub-communities is a new functionality within the CV-Muzar that intends to bring the participants together according to two scopes: interest similarity and knowledge complementarities.

  17. An amoeboid olivine inclusion (AOI) in CK3 NWA 1559, comparison to AOIs in CV3 Allende, and the origin of AOIs in CK and CV chondrites

    NASA Astrophysics Data System (ADS)

    Rubin, Alan E.

    2013-03-01

    An amoeboid olivine inclusion in CK3 NWA 1559 (0.54 × 1.3 mm) consists of a diopside-rich interior (approximately 35 vol%) and an olivine-rich rim (approximately 65 vol%). It is the first AOI to be described in CK chondrites; the apparent paucity of these inclusions is due to extensive parent-body recrystallization. The AOI interior contains irregular 3-15 ?m-sized Al-bearing diopside grains (approximately 70 vol%), 2-20 ?m-sized pores (approximately 30 vol%), and traces of approximately 2 ?m plagioclase grains. The 75-160 ?m-thick rim contains 20-130 ?m-sized ferroan olivine grains, some with 120º triple junctions. A few coarse (25-50 ?m-sized) patches of plagioclase with 2-18 ?m-thick diopside rinds occur in several places just beneath the rim. The occurrence of olivine rims around AOI-1 and around many AOIs in CV3 Allende suggests that CK and CV AOIs formed by the acquisition of porous forsteritic rims around fine-grained, rimless CAIs that consisted of diopside, anorthite, melilite, and spinel. Individual AOIs in carbonaceous chondrites may have formed after transient heating events melted their olivine rims as well as portions of the underlying interiors. In AOI-1, coarse plagioclase grains with diopside rinds crystallized immediately below the olivine rim. Secondary parent-body alteration transformed forsterite in the rims of CV and CK AOIs into more-ferroan olivine. Some of the abundant pores in the interior of AOI-1 may have formed during aqueous alteration after fine-grained melilite and anorthite were leached out. Chondrite groups with large chondrules tend to have large AOIs. AOIs that formed in dust-rich nebular regions (where CV and CK chondrites later accreted) tend to be larger than AOIs from less-dusty regions.

  18. CAMSHIFT Tracker Design Experiments with Intel OpenCV and SAI

    E-print Network

    Southern California, University of

    CAMSHIFT Tracker Design Experiments with Intel OpenCV and SAI Alexandre R.J. Fran¸cois Institute, robustness and, if possible, non-intrusiveness. In order for computer vision techniques to have a significant's Open Computer Vision library and SAI (Software Architecture for Immersipresence), a software

  19. SEPARATION OF SUNLIGHT AND TEMPERATURE EFFECTS ON THE COMPOSITION OF VITIS VINIFERA CV. MERLOT BERRIES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Anthocyanin and phenolic profiles of berry skins from Vitis vinifera cv. Merlot in the Yakima Valley of Washington were influenced by sun exposure and temperature in 1999 and 2000. Total skin monomeric anthocyanin (TSMA) concentrations were higher in 2000 than in 1999 in any given treatment. Berry t...

  20. Evan L. Weissman, CV, January 2015 Page 1 of 9 Evan L. Weissman

    E-print Network

    McConnell, Terry

    in Environmental Policy) University of Tennessee Thesis: Food Security in the 21st Century: Lessons from CubanEvan L. Weissman, CV, January 2015 Page 1 of 9 Evan L. Weissman Assistant Professor of Food Studies Coordinator, Minor in Food Studies Department of Public Health, Food Studies, and Nutrition David B. Falk

  1. Fayalite in the Vigarano CV3 carbonaceous chondrite: Occurrences, formation age and conditions

    E-print Network

    Rhoads, James

    of CV3 chondrites. The fayalites (FaN 80) with grain sizes typically b20 µm occurs in veins that extend/ or magnetite. The fayalite-bearing veins terminate at the boundaries of clasts. No evidence of strong impact enough to make melt veins is found in materials adjacent to the veins. These observations suggest

  2. Indirect measurements of Brachiaria brizantha cv. Marandu fermentable cell wall sugars for second generation biofuels production.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Results of a study conducted to evaluate the possibility of using IVDMD values of B. brizantha cv. Marandu to predict cell wall sugars that would be available in a biorefinery for ethanol production are reported. The study was conducted based on the similarity between rumen enzymes and those used i...

  3. Oxygen isotope systematics of chondrules in the Allende CV3 chondrite: High precision ion microprobe studies

    E-print Network

    Meyers, Stephen R.

    Oxygen isotope systematics of chondrules in the Allende CV3 chondrite: High precision ion in revised form 21 September 2011 Abstract The oxygen three-isotope systematics of 36 chondrules from. However, most relict olivine grains show oxygen iso- tope ratios that overlap with those in homogeneous

  4. arXiv:math.CV/0410109v26Oct2004 WEIGHTED BERGMAN KERNELS AND VIRTUAL BERGMAN

    E-print Network

    arXiv:math.CV/0410109v26Oct2004 WEIGHTED BERGMAN KERNELS AND VIRTUAL BERGMAN KERNELS GUY ROOS Abstract. We introduce the notion of virtual Bergman kernel and study some of its applications. Contents Introduction 1 1. Virtual Bergman kernels 2 2. Virtual Bergman kernels for bounded symmetric domains 7 3

  5. R.A. Schultz, CV; page 1 RICHARD A. SCHULTZ, Ph.D., P.G.

    E-print Network

    ), and deepwater assets including Alaska, Canada, US (Lower 48) and Gulf of Mexico, Norway/North Sea, Russia, LibyaR.A. Schultz, CV; page 1 RICHARD A. SCHULTZ, Ph.D., P.G. 13831 Oak Fair Bend, Cypress, Texas 77429 Registered and licensed as a Professional Geologist (P.G.), Texas Board of Professional Geoscientists, State

  6. Phytoremediation of 4,4'-thiodiphenol (TDP) and other bisphenol derivatives by Portulaca oleracea cv.

    PubMed

    Okuhata, Hiroshi; Ninagawa, Masahiko; Takemoto, Naomichi; Ji, Hezhe; Miyasaka, Hitoshi; Iwamoto, Ai; Nagae, Masaki; Ishibashi, Yasuhiro; Arizono, Koji

    2013-01-01

    4,4'-Thiodiphenol (TDP) is a bisphenol derivative, and there has been no report on TDP removal by any plants or pure bacterial cultures. The removal of TDP by Portulaca oleracea cv., a floricultural herbal plant, was examined with a hydroculture system, and 97% of TDP was removed after 4 days culture. PMID:23040992

  7. THE VALUE OF LUPINUS ALBUS L. CV. AU HOMER AS A WINTER COVER CROP FOR COTTON

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Successful cotton (Gossypium hirsutum L.) management in the southeastern USA with conservation tillage requires the utilization of winter cover crops to increase organic matter in the top 5 cm of the soil. The objective of our research was to test the newly-developed bitter white lupin cv. `AU Home...

  8. Abstract Morphemes and Lexical Representation: The CV-Skeleton in Arabic

    ERIC Educational Resources Information Center

    Boudelaa, Sami; Marslen-Wilson, Willian D.

    2004-01-01

    Overlaps in form and meaning between morphologically related words have led to ambiguities in interpreting priming effects in studies of lexical organization. In Semitic languages like Arabic, however, linguistic analysis proposes that one of the three component morphemes of a surface word is the CV-Skeleton, an abstract prosodic unit coding the…

  9. Outburst of CV ROTSE3 J031031.4+431115.0

    NASA Astrophysics Data System (ADS)

    Dhungana, G.; Ferrante, F. V.; Staten, R.; Kehoe, R.

    2015-02-01

    Further to ATel#1272, we report observations of an outburst of the U Geminorum-type CV ROTSE3 J031031.4+431115.0 in unfiltered CCD images taken by the 0.45 m ROTSE-IIIb telescope at McDonald Observatory, Texas.

  10. LETTER Communicated by Bard Ermentrout Analytic Expressions for Rate and CV of a Type I Neuron

    E-print Network

    Longtin, André

    -driven leaky integrate-and-fire neuron. We focus on the firing rate and coefficient of variation (CV "bias cur- rent" input parameter, a model that can be looked upon as the simplest version of a type I cases of the model. We also show that caution must be used to extend these results to the neuron model

  11. Alian Wang: CV (July 2015) Curriculum Vita of Prof. Alian Wang

    E-print Network

    (Astrobiology Science and Technology for Exploring Planets) project, "life in Atacama". 2011­ 2012, PI, a NASA Development Program) project. #12;Alian Wang: CV (July 2015) 2 2007­2018, PI, a NASA Mars Scout-MoO (Mission-ASTID (Astrobiology Science and Technology Instrument Development) projects, for developing WIR and BUF sensors. 200

  12. CV dtaill et rapport d'activit de Lapo BOSCHI Lapo BOSCHI

    E-print Network

    Boschi, Lapo

    CV détaillé et rapport d'activité de Lapo BOSCHI 1 Lapo BOSCHI Maitre de conférences Laboratoire iSTeP, UMR 7193 UPMC-CNRS Université Pierre et Marie Curie 4, place Jussieu Case 129, T.46-00, Et.2 75252 Paris Cedex 05 Téléphone : +33(0)144275243 Email : lapo

  13. Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The distribution of a potential biological agent, the newly discovered leafhopper-infecting virus, HoCV-1 was examined across the USA. Few biological agents exist for use against leafhopper pests. We examined and compared leafhopper salivary gland and midgut tissues using transmission electron micro...

  14. Chapter C-V-1 Image Formation and Waveform Design for Distributed Apertures in Multipath

    E-print Network

    Yazici, Birsen

    system distributed aperture radar system. We assume that the system is operating in multi for range-Doppler imaging developed in [1,2] to distributed aperture radar systems. Our approach utilizesChapter C-V-1 Image Formation and Waveform Design for Distributed Apertures in Multipath via Gram

  15. Automatic tracking of red blood cells in micro channels using OpenCV

    NASA Astrophysics Data System (ADS)

    Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

    2013-10-01

    The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

  16. Antibiotic targeting of the bacterial secretory pathway Smitha Rao C.V. a

    E-print Network

    Economou, Tassos

    Review Antibiotic targeting of the bacterial secretory pathway Smitha Rao C.V. a , Evelien De xxxx Keywords: Antibiotic target Protein secretion inhibitor Signal peptidase SecA Antibiotic resistance Finding new, effective antibiotics is a challenging research area driven by novel approaches

  17. CV Konrad Tollmar Home adress: 62 Stedman St, Brookline, MA 02446, USA

    E-print Network

    Tollmar, Konrad

    CV Konrad Tollmar Home adress: 62 Stedman St, Brookline, MA 02446, USA Phone home: +1 617 738 1084 and the Oxygen project. Employments 1988-1989 Ericsson Information System 1989-1991 Naturens Hus (A multimedia and Public Spaces - Video Mediated Communication in a Future Home Environment, Video paper at CHI'2000, ACM

  18. Sustainability and its Economic Interpretations John C.V. Pezzey and Michael A. Toman

    E-print Network

    Pezzey, Jack

    1 Chapter 6 Sustainability and its Economic Interpretations by John C.V. Pezzey and Michael A 1987) popularized the concept of sustainability, the idea (if not the precise term) figured prominently is an attempt to determine more precisely under what conditions a sustainable level of per capita consumption

  19. Identification of HoCV-1 virus in Texas Glassy-Winged Sharpshooter Populations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A new strain of leafhopper virus was discovered in leafhoppers which are vectors of Pierce’s disease of grapes. The original leafhopper infecting virus, HoCV-1, was isolated from the glassy-winged sharpshooter, Homalodisca vitripennis, from samples collected in California and Florida. Using the orig...

  20. EST-SSR MARKERS FROM FRAGARIA VESCA L. CV. YELLOW WONDER

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fourteen microsatellite primer pairs were developed from a cDNA library of Fragaria vesca cv. Yellow Wonder. Transferability to 13 species of Fragaria ranged from 71% in diploid species F. gracilis Losinsk., F. iinumae Makino, F. nilgerrensis Schltdl. ex J. Gay and F. nipponica Makino to 100% in the...

  1. Thermal degradation of anthocyanins from purple potato (Cv. Purple Majesty) and their impact on antioxidant capacity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Degradation parameters of purified anthocyanins from purple-fleshed potato (Purple Majesty cv.) heated at high temperatures (100 - 150 °C) was determined. Purified anthocyanins, prepared by removing salts, sugars and colorless non-anthocyanin phenolics from the crude extract, were quantified using H...

  2. Safe, Effective and Easily Reproducible Fusion Technique for CV Junction Instability

    PubMed Central

    Sannegowda, Raghavendra Bakki

    2015-01-01

    Introduction: The Craniovertebral junction (CVJ) refers to a bony enclosure where the occipital bone surrounds the foramen magnum, the atlas and the axis vertebrae. Because of the complexity of structures, CVJ instability is associated with diagnostic and therapeutic problems. Posterior CV fusion procedures have evolved a lot over the last couple of decades. There has been a lookout for one such surgical procedure which is inherently safe, simple, easily reproducible and biomechanically sound. In our study, we present the initial experience the cases of CV junction instrumentation using O-C1-C2 screw & rod construct operated by the author. Aims and Objectives: The current study is a descriptive analysis of the cases of CVJ instability treated by us with instrumentation using O-C1-C2 screw and rod construct fusion technique. Materials and Methods: It is a retrospective, analytical study in which cases of CV junction instability operated by the author between January 2010 to March 2014 were analysed using various clinical, radiological and outcome parameters. Conclusion: CV junction instrumentation using O-C1-C2 screw and rod construct fusion technique proved to be safe, effective, easily reproducible and biomechanically sound technique which can be adopted by all surgeons who may be at any stage of their learning curve. PMID:25954660

  3. M. Bahrami Fluid Mechanics (S 09) Integral Relations for CV 8 The angular momentum

    E-print Network

    Bahrami, Majid

    M. Bahrami Fluid Mechanics (S 09) Integral Relations for CV 8 The angular momentum A control volume analysis can be applied to the angular momentum, by letting B equal to angular momentum vector H. If O is the point about which moments are desired, the angular moment about O

  4. Formation timescales of CV chondrites from component specific Hf-W systematics

    NASA Astrophysics Data System (ADS)

    Becker, Maike; Hezel, Dominik C.; Schulz, Toni; Elfers, Bo-Magnus; Münker, Carsten

    2015-12-01

    Carbonaceous chondrites are an important meteorite group that closely resembles the bulk composition of the solar system. We report the first elemental and isotope dataset for Hf-W in carbonaceous chondrites that includes chondrules, matrix, magnetic fractions as well as bulk compositions. Our study focuses on the three CV3 chondrites, Allende, Vigarano and Bali. Compared to bulk chondrites, matrix splits have low Hf/W ratios and ?182W compositions, whereas chondrule splits are characterized by high, but more variable, Hf/W ratios and ?182W compositions. Thus, Hf/W ratios behave complementary between chondrules and matrix in the analysed CV chondrites, supporting the view that both components formed from the same parental reservoir. Strong nucleosynthetic effects were observed in most of the analysed CV3 components, especially in matrices and chondrule splits that were found to have large ?183W anomalies of several ?-units. All separates define a rough correlation between initial 182W/184W and 183W/184W ratios, in agreement with theoretical model trends based on calculations for stellar nucleosynthesis. Our results, therefore, indicate a heterogeneous distribution of s- and r-process W isotopes among the different CV3 chondrite components, arguing for selective thermal processing of early solar system matter during chondrule formation. After correcting for nucleosynthetic anomalies, chondrules and matrix splits of reduced (Vigarano) as well as oxidised (Allende) CV3 chondrites define a linear correlation in ?182W vs. 180Hf/184W space, which is interpreted as an isochron, covering an age interval within the first ?2.6 Ma after solar system formation. As peak metamorphic temperatures for CV3 chondrites were well below the 182Hf-182W closure temperature, the resulting isochron within its error most likely defines a common formation interval for all components. The calculated age interval is for the first time based on a combined chondrule-matrix isochron, a marked difference compared to previous studies where only chondrules were analysed. Notably, our formation age interval covers previously reported chondrule formation ages determined using 26Al and Pb-Pb chronometry, illustrating that chondrule and matrix formation started contemporaneously with CAI formation and lasted over a time interval of about 2-3 Ma. Our results also corroborate previous models from ordinary chondrites, in that chondrite parent bodies were not the first planetesimals to have formed in the early solar system.

  5. Formation of biphenyl and dibenzofuran phytoalexins in the transition zones of fire blight-infected stems of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference'.

    PubMed

    Chizzali, Cornelia; Khalil, Mohammed N A; Beuerle, Till; Schuehly, Wolfgang; Richter, Klaus; Flachowsky, Henryk; Peil, Andreas; Hanke, Magda-Viola; Liu, Benye; Beerhues, Ludger

    2012-05-01

    In the rosaceous subtribe Pyrinae (formerly subfamily Maloideae), pathogen attack leads to formation of biphenyls and dibenzofurans. Accumulation of these phytoalexins was studied in greenhouse-grown grafted shoots of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference' after inoculation with the fire blight bacterium, Erwinia amylovora. No phytoalexins were found in leaves. However, both classes of defence compounds were detected in the transition zone of stems. The flanking stem segments above and below this zone, which were necrotic and healthy, respectively, were devoid of detectable phytoalexins. The transition zone of apple stems contained the biphenyls 3-hydroxy-5-methoxyaucuparin, aucuparin, noraucuparin and 2'-hydroxyaucuparin and the dibenzofurans eriobofuran and noreriobofuran. In pear, aucuparin, 2'-hydroxyaucuparin, noreriobofuran and in addition 3,4,5-trimethoxybiphenyl were detected. The total phytoalexin content in the transition zone of pear was 25 times lower than that in apple. Leaves and stems of mock-inoculated apple and pear shoots lacked phytoalexins. A number of biphenyls and dibenzofurans were tested for their in vitro antibacterial activity against some Erwinia amylovora strains. The most efficient compound was 3,5-dihydroxybiphenyl (MIC=115 ?g/ml), the immediate product of biphenyl synthase which initiates phytoalexin biosynthesis. PMID:22377689

  6. Graphical method for determining the coefficient of consolidation cv from a flow-pump permeability test

    USGS Publications Warehouse

    Morin, Roger H.; Olsen, Harold W.; Nelson, Karl R.; Gill, James D.

    1989-01-01

    A graphical method has been developed for determining the coefficient of consolidation from the transient phases of a flow-pump permeability test. The flow pump can be used to infuse fluid into or withdraw fluid from a laboratory sediment specimen at a constant volumetric rate in order to obtain data that can be used to calculate permeability using Darcy's law. Representative type-curve solutions to the associated forced-flow and pressure-decay models are derived. These curves provide the basis for graphically evaluating the permeability k, the coefficient of consolidation cv, and the coefficient of volume change mv. The curve-matching technique is easy and rapid. Values of k, cv and mv for a laterally confined kaolinite specimen were determined by this graphical method and appear to be in reasonably good agreement with numerically derived estimates (within 20%). Discrepancies between the two sets of results seem to be largely a function of data quality.

  7. Xoconostle fruit (Opuntia matudae Scheinvar cv. Rosa) by-products as potential functional ingredients.

    PubMed

    Morales, Patricia; Barros, Lillian; Ramírez-Moreno, Esther; Santos-Buelga, Celestino; Ferreira, Isabel C F R

    2015-10-15

    There is a lack of information on the potential use of xoconostle cultivars as sources of antioxidants for food, pharmaceutical and colorant industries. The aim of this study was to provide a phytochemical characterisation and antioxidant activity evaluation of Opuntia matudae Scheinvar cv. Rosa by-products (epicarp and endocarp mucilage's), in order to evaluate their interest as sources of functional ingredients for human or animal foods. These by-products showed a high content in glucose, citric and linoleic acids, tocopherols, and isorhamnetin-O-(di-deoxyhexosyl-hexoside) (mainly in epicarp), and presented relevant antioxidant properties. The obtained results support the use of O. matudae Scheinvar cv. Rosa agro-industrial by-products as functional food ingredients, namely for antioxidant-enriched formulations, instead of being discarded. PMID:25952871

  8. Camera calibration method of binocular stereo vision based on OpenCV

    NASA Astrophysics Data System (ADS)

    Zhong, Wanzhen; Dong, Xiaona

    2015-10-01

    Camera calibration, an important part of the binocular stereo vision research, is the essential foundation of 3D reconstruction of the spatial object. In this paper, the camera calibration method based on OpenCV (open source computer vision library) is submitted to make the process better as a result of obtaining higher precision and efficiency. First, the camera model in OpenCV and an algorithm of camera calibration are presented, especially considering the influence of camera lens radial distortion and decentering distortion. Then, camera calibration procedure is designed to compute those parameters of camera and calculate calibration errors. High-accurate profile extraction algorithm and a checkboard with 48 corners have also been used in this part. Finally, results of calibration program are presented, demonstrating the high efficiency and accuracy of the proposed approach. The results can reach the requirement of robot binocular stereo vision.

  9. GC-FID/MS Profiling of Supercritical CO2 Extracts of Peels from Citrus aurantium, C. sinensis cv. Washington navel, C. sinensis cv. Tarocco and C. sinensis cv. Doppio Sanguigno from Dubrovnik Area (Croatia).

    PubMed

    Jerkovi?, Igor; Drulži?, Jasmina; Marijanovi?, Zvonimir; Gugi?, Mirko; Joki?, Stela; Roje, Marin

    2015-07-01

    The peels of Citrus aurantium L. and Citrus sinensis Osbeck cultivars from the Dubrovnik region (south Croatia) were extracted by supercritical CO2 at 40 degrees C and 10 MPa at 1.76 kg/h to obtain enriched extracts in comparison with simple pressing of the peels. The extracts were analyzed in detail by gas chromatography and mass spectrometry (GC-FID/MS). Relevant similarities among the peel oil compositions of C. aurantium and C. sinensis cultivars were found with limonene predominance (up to 54.3%). The principal oxygenated monoterpenes were linalool (3.0%-5.9%), ?-terpineol (0.7%-2.4%), linalyl acetate (0.0%-5.0%), geranyl acetate (0.0%-0.4%), (Z)-citral (0.0%-1.8%) and (E)-citral (0.0%-1.9%). Several sesquiterpenes were found with minor percentages. Coumarin derivatives were identified in all the samples among the relevant compounds. Isogeijerin dominated in the peels of C. sinensis cv. Tarocco (15.3%) and C. aurantium (11.2%). Scoparone ranged from 0.1% to 0.5% in all the samples. Bergapten (up to 1.4%), osthole (up to 1.1%) and 7-methoxy-8-(2-formylpropyl)coumarin (up to 1.1%) were found mostly in C. sinensis cv. Doppio Sanguigno. It was possible to indicate a few other differences among the extracts such as higher percentage of linalool, linalyl and geranyl acetates, as well as the abundance of sabinene and isogeijerin in C. aurantium or the occurrence of ?-sinensal in C. sinensis cultivars. PMID:26411039

  10. IV and CV curves for irradiated prototype BTeV silicon pixel sensors

    SciTech Connect

    Maria R. Coluccia et al.

    2002-07-16

    The authors present IV and CV curves for irradiated prototype n{sup +}/n/p{sup +} silicon pixel sensors, intended for use in the BTeV experiment at Fermilab. They tested pixel sensors from various vendors and with two pixel isolation layouts: p-stop and p-spray. Results are based on exposure with 200 MeV protons up to 6 x 10{sup 14} protons/cm{sup 2}.

  11. Biochemical analysis of SV40 small t mediated theophylline resistance in CV-1 cells

    SciTech Connect

    Renz, C.D.

    1986-01-01

    The papovavirus SV40 encodes for the two tumor antigens, large T and small t. While much is known about large T, little information is available about the role of small t in the viral life cycle. The authors have developed a system for studying small t antigen based on its ability to overcome the G/sub 0/ growth arrest induced by the methylxanthine, theophylline. Uninfected CV-1 cells, the permissive host for SV40, are arrested by 1-2mM theophylline. In contrast, Wt-infected cells are not arrested by the same concentrations of this drug. Biochemical studies were designed to analyze the effects of theophylline and the means by which small t can overcome the growth arrest of CV-1 cells. Theophylline, a cyclic AMP analogue, does not appear to arrest CV-1 cells by a cAMP-dependent mechanism. Theophylline appears to arrest CV-1 cells by inhibiting sodium influx. Both /sub 86/Rb/sup +/ and /sup 22/Na/sup +/ uptake were inhibited by theophylline. Amiloride and TMB-8, drugs which are known to inhibit the plasma membrane Na/sup +//H/sup +/ antiporter, decreased /sup 86/Rb/sup +/ and /sup 22/Na/sup +/ uptake to the same degree as theophylline. Because these drugs also arrested mock and D1- but not Wt-infected cells it is possible that theophylline inhibits sodium uptake by inhibiting this antiporter. Furthermore, because Wt-infected cells are resistant to the growth arrest induced by these drugs, it is possible that small t acts either by directly altering this antiporter or by bypassing the step which requires the activity of the antiporter.

  12. CV population densities: Using CFHT as the worlds largest time-series photometer

    NASA Astrophysics Data System (ADS)

    Lott, D. A.; Haswell, C. A.; Abbott, T. M. C.; Ringwald, F.

    2002-01-01

    Known samples of cataclysmic variables (CVs) are sparse and/or rife with selection effects and do not provide adequate tests of theories of their evolution. We have used the CFHT12k mosaic camera as the worlds largest time-series photometer, observing a 0.3 sq.deg. Galactic field with 3 minute time-resolution. The resulting unbiased, magnitude-limited sample of CVs, and their progenitors will be used to test the predictions of CV population models.

  13. Numerical C-V calculations of CdTe/CdS thin films solar cell

    NASA Astrophysics Data System (ADS)

    Castillo-Alvarado, F. L.; Avendaño-Lopez, J.; Contreras-Puente, G.; Vigil, O.; Vidal, J.; de Gante, P.; Rojas-Hernandez, E.; Hernandez-Contreras, H.

    2000-03-01

    We have calculated the band discontinuites and interface charge density of the heterojunction CdTe/CdS, the calculations are based in the C-V matching method. Our solar cells are builted with thin films done by close spaced sublimation. The significance of the simultaneous determinations of the band discontinuites and interface charges of these solar cells is discussed. Work partially supported by CONACYT, Mexico, proyect G27713A

  14. A real-time camera calibration system based on OpenCV

    NASA Astrophysics Data System (ADS)

    Zhang, Hui; Wang, Hua; Guo, Huinan; Ren, Long; Zhou, Zuofeng

    2015-07-01

    Camera calibration is one of the essential steps in the computer vision research. This paper describes a real-time OpenCV based camera calibration system, and developed and implemented in the VS2008 environment. Experimental results prove that the system to achieve a simple and fast camera calibration, compared with MATLAB, higher precision and does not need manual intervention, and can be widely used in various computer vision system.

  15. Teaching image processing and pattern recognition with the Intel OpenCV library

    NASA Astrophysics Data System (ADS)

    Koz?owski, Adam; Królak, Aleksandra

    2009-06-01

    In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image processing and pattern recognition with the most up-to-date solutions available. In the Institute of Electronics at the Technical University of Lodz we facilitate the teaching process in this subject with the OpenCV library, which is an open-source set of classes, functions and procedures that can be used in programming efficient and innovative algorithms for various purposes. The topics of student projects completed with the help of the OpenCV library range from automatic correction of image quality parameters or creation of panoramic images from video to pedestrian tracking in surveillance camera video sequences or head-movement-based mouse cursor control for the motorically impaired.

  16. Utrophins compensate for Dp71 absence in mdx3cv in adhered platelets.

    PubMed

    Cerecedo, Doris; Mondragón, Ricardo; Candelario, Aurora; García-Sierra, Francisco; Mornet, Dominique; Rendón, Alvaro; Martínez-Rojas, Dalila

    2008-01-01

    Platelet adhesion is a critical step due to its hemostatic role in stopping bleeding after vascular damage. Short dystrophins are the most abundant dmd gene products in nonmuscle tissues, and in association with cytoskeleton proteins contribute to their intrinsic function; while utrophins are dystrophin-homologous related family proteins with structural and functional similarities. We previously demonstrated the presence of Dp71 isoforms, utrophins, and various dystrophin-associated proteins and their participation in cytoskeleton re-organization, filopodia and lamellipodia extension, and in centralizing cytoplasmic granules during the adhesion process of human platelets. To evaluate the morphologic changes and actin-based structures of mdx(3cv) platelets during the adhesion process, we compared the topographic distribution of Dp71d/Dp71Delta110(m) and dystrophin-associated protein in adhered platelets from dystrophic mdx(3cv) mouse. By confocal microscopy, we showed that absence of Dp71 isoforms in platelets from this animal model disrupted dystrophin-associated protein expression and distribution without modifying the platelet morphology displayed during the glass-adhesion process. By immunoprecipitation assays, we proved that up-regulated utrophins were associated with dystrophin-associated proteins to conform the dystrophin-associated protein complex corresponding to utrophins, which might compensate for Dp71 absence in mdx(3cv) platelets. PMID:18180614

  17. Thermal performance of a top heat mode closed-loop oscillating heat pipe with a check valve (THMCLOHP/CV)

    NASA Astrophysics Data System (ADS)

    Bhuwakietkumjohn, N.; Parametthanuwat, T.

    2015-05-01

    The aim of this research is to investigate the heat transfer characteristics of a top heat mode closed-looped oscillating heat pipe with check valves (THMCLOHP/CV). Ethanol is used as a working fluid with filling ratios of 30, 50, and 80% of the total volume of the tube. The THMCLOHP/CV is made of a copper tube with an inside diameter of 2.03 mm. The angle of inclination is 90° from the horizontal axis with 40 turns, two check valves, and an evaporator length of 50, 100, and 150 mm. The operating temperatures are 44 and 55°C. It is found that the thermal resistance decreases significantly as the working temperature is increased. Thus, the evaporator length affects the thermal resistance of the THMCLOHP/CV. The presence of the THMCLOHP/CV is clearly demonstrated to contribute to thermal performance improvement.

  18. Compositional and petrographic similarities of CV and CK chondrites: A single group with variations in textures and volatile concentrations attributable to impact heating, crushing and oxidation

    NASA Astrophysics Data System (ADS)

    Wasson, John T.; Isa, Junko; Rubin, Alan E.

    2013-05-01

    Greenwood et al. (2010) gathered data on O-isotopic and elemental compositions and reevaluated literature data for CV and CK chondrites. They concluded that these two chondrite groups originated on the same parent asteroid, with CK chondrites being metamorphosed CV chondrites (which are otherwise missing types 4 through 6). To test this interpretation we have gathered new instrumental neutron-activation-analysis (INAA) data for CV and CK chondrites and reexamined their petrographic features. The new INAA data like the older data show scatter attributable to weathering effects, but we conclude that the refractory lithophile abundances are the same in CV and CK, in agreement with the Greenwood et al. interpretation. Several volatile elements are significantly lower in CK than in CV chondrites. Among the elements we determine, the greatest difference between CV and CK is found for Br, for which the CV/CK ratio is ?4; As and Sb are about 20% lower in CK than CV and smaller differences are observed for Zn, Ga and Se. It seems likely that volatiles were lost during impact-heating events that also provided the heat responsible for metamorphic recrystallization. Within statistical uncertainty, chondrules in CV and CK chondrites are the same size and have similar textural distributions. A significant petrographic difference between CK and CV chondrites cited by Kallemeyn et al. (1991) was the much higher percentage of igneous rims around CV chondrules. However, we now recognize that many chondrules in CK3.8 NWA 1559 have igneous rims and in CK4 chondrites, igneous rims are recognizable by their associated sulfide-rich rings; there are no quantifiable CV-CK differences in igneous-rim abundances. We used Ca and Al maps to show that CK chondrites have CAI abundances similar to those of CV chondrites. It thus appears that there are no resolvable pre-metamorphic petrographic differences between CV and CK chondrites. We recommend that the “CK” designation be abandoned and that the CV group be acknowledged as spanning the range of petrologic types 3-6. We suggest that CK3 chondrites be designated CV3OxK. Most CK4 chondrites are highly fragmental; collisional crushing appears to be much more common than in CV chondrites. It seems likely that CK chondrites formed from reduced CV3 materials after the latter was impacted, buried, aqueously altered and annealed.

  19. Complete CV

    E-print Network

    2015-12-13

    University education. Studies of physics .... Ad hoc committee for strategic planning (interdiscipliary, equipment). Spr 06. > Ad hoc .... ESF conference on Teichmüller Theory and its Interactions in Mathematics and Physics,. Jun 10. Bellaterra ...

  20. The first mass and angular momentum loss measurements for a CV-like binary

    NASA Astrophysics Data System (ADS)

    Drake, Jeremy

    2014-10-01

    The period distribution of close binaries, cataclysmic variables, novae and single-degenerate SN1a progenitor candidates is largely controlled by magnetically-driven mass and angular momentum loss (AML) from the M dwarf secondary. The mass loss rates for these spun-up stars remain essentially unknown and impossible to observe directly, with likely values in the range 1e-12 to 1e-15 Msun/yr. AML presciptions for CVs differ by orders of magnitude. One way to measure the mass loss rate is to observe the dM wind accrete onto its WD companion in a pre-CV very close to Roche Lobe overflow but lacking the obscuring complications and emission from an accretion disk. The measurement can be combined with realistic MHD models to understand the accretion fraction, the mass that escapes, and the AML. The best-studied nearby pre-CV is QS Vir (48pc, P=3.6hr). However, its wind accretion rates measured from 1999 HST UV spectra of the WD metal absorption lines and 2006 XMM-Newton CCD spectroscopy differ by a factor of a thousand, pointing to either a dominant CME stochastic component, or a "magnetic switch" found in MHD simulations and driven by cyclic activity on the M dwarf. HST COS spectra combined with XMM-Newton monitoring on timescales from weeks to years will tease out CME vs cyclic accretion variations. UV and X-ray measurements will provide the first consistency check of both accretion rate measurement methods. MHD models tailored to the system will enable the first quasi-direct measurements of the mass loss and AML from a CV-like binary. Our project requires 6 HST/COS orbits in Cycles 22-24, and 60ksec on XMM in Cycle 22

  1. Pulmonary nodule detection in CT images based on shape constraint CV model

    SciTech Connect

    Wang, Bing; Tian, Xuedong; Wang, Qian; Yang, Ying; Xie, Hongzhi E-mail: xiehongzhi@medmail.com.cn; Zhang, Shuyang; Gu, Lixu E-mail: xiehongzhi@medmail.com.cn

    2015-03-15

    Purpose: Accurate detection of pulmonary nodules remains a technical challenge in computer-aided diagnosis systems because some nodules may adhere to the blood vessels or the lung wall, which have low contrast compared to the surrounding tissues. In this paper, the analysis of typical shape features of candidate nodules based on a shape constraint Chan–Vese (CV) model combined with calculation of the number of blood branches adhered to nodule candidates is proposed to reduce false positive (FP) nodules from candidate nodules. Methods: The proposed scheme consists of three major stages: (1) Segmentation of lung parenchyma from computed tomography images. (2) Extraction of candidate nodules. (3) Reduction of FP nodules. A gray level enhancement combined with a spherical shape enhancement filter is introduced to extract the candidate nodules and their sphere-like contour regions. FPs are removed by analysis of the typical shape features of nodule candidates based on the CV model using spherical constraint and by investigating the number of blood branches adhered to the candidate nodules. The constrained shapes of CV model are automatically achieved from the extracted candidate nodules. Results: The detection performance was evaluated on 127 nodules of 103 cases including three types of challenging nodules, which are juxta-pleural nodules, juxta-vascular nodules, and ground glass opacity nodules. The free-receiver operating characteristic (FROC) curve shows that the proposed method is able to detect 88% of all the nodules in the data set with 4 FPs per case. Conclusions: Evaluation shows that the authors’ method is feasible and effective for detection of three types of nodules in this study.

  2. Flight test results from the CV990 simulated space shuttle during unpowered automatic approaches and landings

    NASA Technical Reports Server (NTRS)

    Edwards, F. G.; Foster, J. D.

    1973-01-01

    Unpowered automatic approaches and landings with a CV990 aircraft were conducted to study navigation, guidance, and control problems associated with terminal area approach and landing for the space shuttle. The flight tests were designed to study from 11,300 m to touchdown the performance of a navigation and guidance concept which utilized blended radio/inertial navigation using VOR, DME, and ILS as the ground navigation aids. In excess of fifty automatic approaches and landings were conducted. Preliminary results indicate that this concept may provide sufficient accuracy to accomplish automatic landing of the shuttle orbiter without air-breathing engines on a conventional size runway.

  3. Dark inclusions in Allende, Leoville, and Vigarano - Evidence for nebular oxidation of CV3 constituents

    NASA Technical Reports Server (NTRS)

    Johnson, Craig A.; Prinz, Martin; Weisberg, Michael K.; Clayton, Robert N.; Mayeda, Toshiko K.

    1990-01-01

    The origin and the history of dark inclusions (DIs) are investigated using petrologic, chemical, and oxygen isotopic data on ten DI samples from Allende, Leoville, and Vigarano. These data indicate that the DIs of the Leoville and Vigarano are closely similar to those of Allende. The inclusions appear to be fragments of CV3 parent bodies which were processed to different degrees prior to their incorporation as clasts into the Allende, Leoville, and Vigarano chondrites. The processing homogenized the olivine compositions, presumably through heating, and also involved oxygen exchange with O-16-poorer surroundings.

  4. The light curve of CV Serpentis, the sometimes-eclipsing Wolf-Rayet star

    NASA Technical Reports Server (NTRS)

    Schild, R.; Liller, W.

    1975-01-01

    New photoelectric observations of the B-magnitude of CV Ser made in 1973 and 1974 show no clear evidence of an eclipse, but they establish night-to-night variability of several percent, a systematic brightness change of 0.035 mag during a portion of the single orbit observed in 1973, and irregular flaring in 1974. We made iris photometer measurements of Harvard patrol plates taken between 1905 June and 1953 July, and find no evidence of a very deep eclipse such as observed by Hjellming and Hiltner. We present several new light curves and discuss then in the light of the recent results of Cowley et al.

  5. Low-lift-to-drag-ratio approach and landing studies using a CV-990 airplane

    NASA Technical Reports Server (NTRS)

    Kock, B. M.; Fulton, F. L.; Drinkwater, F. J., III

    1972-01-01

    The results are presented of a flight-test program utilizing a CV-990 airplane, flow in low-lift-to-drag-ratio (L/D) configurations, to simulate terminal area operation, approach, and landing of large unpowered vehicles. The results indicate that unpowered approaches and landings are practical with vehicles of the size and performance characteristics of the proposed shuttle vehicle. Low L/D landings provided touchdown dispersion patterns acceptable for operation on runways of reasonable length. The dispersion pattern was reduced when guidance was used during the final approach. High levels of pilot proficiency were not required for acceptable performance.

  6. Ultrastructural changes in shoot apical meristem of canola (Brassica napus cv. Symbol) treated with sodium chloride.

    PubMed

    Mahmoodzadeh, Homa

    2008-04-15

    In the present research, structure and ultrastructure of shoot apical meristem of canola (Brassica napus cv. Symbol) under salinity conditions were investigated. The experiments were conducted in five groups (0, 3, 6, 9, 12 dS m(-1)) under greenhouse conditions. Sampling of apical meristem and TEM tissue preparation procedure were carried out. Semithin and ultrathin sections were prepared and viewed in light and electron microscopy, respectively. The results included reduction of meristem size, disorders in meristem structure. Also formation of autophagic vacuoles was observed that is probably one of the plant responses to salt stress for more water storage in these vacuoles and decreasing of cell water requirements. PMID:18819558

  7. Three in one go: consequential angular momentum loss can solve major problems of CV evolution

    NASA Astrophysics Data System (ADS)

    Schreiber, M. R.; Zorotovic, M.; Wijnen, T. P. G.

    2016-01-01

    The average white dwarf (WD) masses in cataclysmic variables (CVs) have been measured to significantly exceed those of single WDs, which is the opposite of what is theoretically expected. We present the results of binary population synthesis models taking into account consequential angular momentum loss (CAML) that is assumed to increase with decreasing WD mass. This approach cannot only solve the WD mass problem but also brings in agreement theoretical predictions and observations of the orbital period distribution and the space density of CVs. We speculate that frictional angular momentum loss following nova eruptions might cause such CAML and could be the missing ingredient of CV evolution.

  8. Three in one go: consequential angular momentum loss can solve major problems of CV evolution

    E-print Network

    Schreiber, Matthias R; Wijnen, Thomas P G

    2015-01-01

    The average white dwarf (WD) masses in cataclysmic variables (CVs) have been measured to significantly exceed those of single WDs, which is the opposite of what is theoretically expected. We present the results of binary population synthesis models taking into account consequential angular momentum loss (CAML) that is assumed to increase with decreasing WD mass. This approach can not only solve the WD mass problem, but also brings in agreement theoretical predictions and observations of the orbital period distribution and the space density of CVs. We speculate that frictional angular momentum loss following nova eruptions might cause such CAML and could thus be the missing ingredient of CV evolution.

  9. Galectin CvGal2 from the Eastern Oyster (Crassostrea virginica) Displays Unique Specificity for ABH Blood Group Oligosaccharides and Differentially Recognizes Sympatric Perkinsus Species.

    PubMed

    Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N; Bachvaroff, Tsvetan R; Tasumi, Satoshi; Pasek, Marta; Banerjee, Aditi; Shridhar, Surekha; Wang, Lai-Xi; Bianchet, Mario A; Vasta, Gerardo R

    2015-08-01

    Galectins are highly conserved lectins that are key to multiple biological functions, including pathogen recognition and regulation of immune responses. We previously reported that CvGal1, a galectin expressed in phagocytic cells (hemocytes) of the eastern oyster (Crassostrea virginica), is hijacked by the parasite Perkinsus marinus to enter the host, where it causes systemic infection and death. Screening of an oyster hemocyte cDNA library revealed a novel galectin, which we designated CvGal2, with four tandemly arrayed carbohydrate recognition domains (CRDs). Phylogentic analysis of the CvGal2 CRDs suggests close relationships with homologous CRDs from CvGal1. Glycan array analysis, however, revealed that, unlike CvGal1 which preferentially binds to the blood group A tetrasaccharide, CvGal2 recognizes both blood group A and B tetrasaccharides and related structures, suggesting that CvGal2 has broader binding specificity. Furthermore, SPR analysis demonstrated significant differences in the binding kinetics of CvGal1 and CvGal2, and structural modeling revealed substantial differences in their interactions with the oligosaccharide ligands. CvGal2 is homogeneously distributed in the hemocyte cytoplasm, is released to the extracellular space, and binds to the hemocyte surface. CvGal2 binds to P. marinus trophozoites in a dose-dependent and ?-galactoside-specific manner. Strikingly, negligible binding of CvGal2 was observed for Perkinsus chesapeaki, a sympatric parasite species mostly prevalent in the clams Mya arenaria and Macoma balthica. The differential recognition of Perkinsus species by the oyster galectins is consistent with their relative prevalence in oyster and clam species and supports their role in facilitating parasite entry and infectivity in a host-preferential manner. PMID:26158802

  10. The Glycine max cv. Enrei Genome for Improvement of Japanese Soybean Cultivars

    PubMed Central

    Shimomura, Michihiko; Kanamori, Hiroyuki; Komatsu, Setsuko; Namiki, Nobukazu; Mukai, Yoshiyuki; Kurita, Kanako; Kamatsuki, Kaori; Ikawa, Hiroshi; Yano, Ryoichi; Ishimoto, Masao; Kaga, Akito; Katayose, Yuichi

    2015-01-01

    We elucidated the genome sequence of Glycine max cv. Enrei to provide a reference for characterization of Japanese domestic soybean cultivars. The whole genome sequence obtained using a next-generation sequencer was used for reference mapping into the current genome assembly of G. max cv. Williams 82 obtained by the Soybean Genome Sequencing Consortium in the USA. After sequencing and assembling the whole genome shotgun reads, we obtained a data set with about 928?Mbs total bases and 60,838 gene models. Phylogenetic analysis provided glimpses into the ancestral relationships of both cultivars and their divergence from the complex that include the wild relatives of soybean. The gene models were analyzed in relation to traits associated with anthocyanin and flavonoid biosynthesis and an overall profile of the proteome. The sequence data are made available in DAIZUbase in order to provide a comprehensive informatics resource for comparative genomics of a wide range of soybean cultivars in Japan and a reference tool for improvement of soybean cultivars worldwide. PMID:26199933

  11. Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO.

    PubMed

    Albert, Andreas; Sareedenchai, Vipaporn; Heller, Werner; Seidlitz, Harald K; Zidorn, Christian

    2009-05-01

    Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity of the tissues involved. Factors causing these variations were investigated by climate chamber (CC) experiments focusing on temperature and ultraviolet (UV)-B radiation. Plants of A. montana L. cv. ARBO were grown in CCs under realistic climatic and radiation regimes. Key factors temperature and UV-B radiation were altered between different groups of plants. Subsequently, flowering heads were analyzed by HPLC for their contents of flavonoids and caffeic acid derivatives. Surprisingly, increased UV-B radiation did not trigger any change in phenolic metabolites in Arnica. In contrast, a pronounced increase in the ratio of B-ring ortho-diphenolic (quercetin) compared to B-ring monophenolic (kaempferol) flavonols resulted from a decrease in temperature by 5 degrees C in the applied climate regime. In conclusion, enhanced UV-B radiation is probably not the key factor triggering shifts in the phenolic composition in Arnica grown at higher altitudes but rather temperature, which decreases with altitude. PMID:19194724

  12. Determination of polyphenols and antioxidant activity of Vitis labrusca cv. baile berries.

    PubMed

    Nile, Shivraj Hariram; Park, Se Won

    2015-10-01

    Grape juice and grape skin extracts are important commercial source of polyphenolic compounds which exert different functional properties such as color potential, antimicrobial, antioxidant activity, and health benefits. In this paper we describe a sensitive and specific assay for determination of bioactive polyphenolic compounds in Campbell Early (Vitis labrusca cv. baile). Five polyphenolic components were separated on an Agilent Zorbax Extend C18 Column (250 mm x 4.6 mm x 5 ?m) and detected by a diode array detector. The mobile phase was composed of (a) aqueous phosphoric acid (0.2%, v/v); and (b) acetonitrile using a gradient elution. Analytes were performed at 25 degrees C with a flow rate of 0.8 ml/min and UV detection at 280, 360, and 520 nm. All calibration curves showed good linear regression (r2 ? 0.9999) within tested ranges. Overall intra- and inter-day variations were less than 1.90%, and the average recoveries were 95.5-105% for analytes. The antioxidant activity determined by DPPH radical assay, ranged from 86-105 for extracts, and 165-252 for studied standards (?M trolox/100 g dry wt.). The proposed method would be sensitive enough and reliable for quality control in functional food and modernization of Campbell Early (Vitis labrusca cv. baile) as potent antioxidant agents. PMID:26665298

  13. Heterogeneous compute in computer vision: OpenCL in OpenCV

    NASA Astrophysics Data System (ADS)

    Gasparakis, Harris

    2014-02-01

    We explore the relevance of Heterogeneous System Architecture (HSA) in Computer Vision, both as a long term vision, and as a near term emerging reality via the recently ratified OpenCL 2.0 Khronos standard. After a brief review of OpenCL 1.2 and 2.0, including HSA features such as Shared Virtual Memory (SVM) and platform atomics, we identify what genres of Computer Vision workloads stand to benefit by leveraging those features, and we suggest a new mental framework that replaces GPU compute with hybrid HSA APU compute. As a case in point, we discuss, in some detail, popular object recognition algorithms (part-based models), emphasizing the interplay and concurrent collaboration between the GPU and CPU. We conclude by describing how OpenCL has been incorporated in OpenCV, a popular open source computer vision library, emphasizing recent work on the Transparent API, to appear in OpenCV 3.0, which unifies the native CPU and OpenCL execution paths under a single API, allowing the same code to execute either on CPU or on a OpenCL enabled device, without even recompiling.

  14. Multi-camera calibration based on openCV and multi-view registration

    NASA Astrophysics Data System (ADS)

    Deng, Xiao-ming; Wan, Xiong; Zhang, Zhi-min; Leng, Bi-yan; Lou, Ning-ning; He, Shuai

    2010-10-01

    For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the corresponding relationship between each camera view, the computation of the rotation matrix and translation matrix is formulated as a constrained optimization problem. According to the Kuhn-Tucker theorem and the properties on the derivative of the matrix-valued function, the formulae of rotation matrix and translation matrix are deduced by using singular value decomposition algorithm. Afterwards an iterative method is utilized to get the entire coordinate transformation of pair-wise views, thus the precise multi-view registration can be conveniently achieved and then can get the relative positions in them(the camera outside the parameters).Experimental results show that the method is practical in multi-camera calibration .

  15. More evidence for a partially differentiated CV chondrite parent body from paleomagnetic studies of ALH 84028 and ALH 85006

    NASA Astrophysics Data System (ADS)

    Klein, B. Z.; Weiss, B. P.; Carporzen, L.

    2014-12-01

    Recent paleomagnetic studies of the CV carbonaceous chondrites Allende and Kaba and numerical modeling studies have suggested that the CV chondrite parent body may have been partially differentiated, with a molten metallic core, dynamo magnetic field, and an unmelted chondritic lid. To further evaluate this hypothesis, here we present new paleomagnetic analyses of two previously unstudied CV3 chondrites: the unshocked, Allende-type oxidized chondrite ALH 84028 and the weakly shocked, Bali-type oxidized chondrite ALH 85006. We preformed alternating field (AF) and thermal demagnetization experiments, AF-based paleointensity experiments, and rock magnetic experiments on mutually oriented subsamples of each meteorite. Both meteorites pass fusion crust baked contact tests, indicating that their interiors retain a magnetization predating atmospheric entry. In the interior of ALH 84028, we identified a unidirectional medium temperature (blocked to 300°C), high coercivity (blocked to >420 mT) component. In the interior of ALH 85006, we identified MT components blocked up to 400-475°C. The unblocking temperatures and unidirectional nature of the MT components in both meteorites indicates their origin as a partial thermoremanence or thermochemical remanence acquired during metamorphism following accretion of the CV chondrite parent body. Our paleointensity experiments indicate paleofield intensities of 32-73 ?T for ALH 84028 and 14-45 ?T for ALH 85006 . When combined with similar recent results for Allende and Kaba, there is now consistent evidence for dynamo fields from four CV chondrites with collectively diverse lithologies and shock states. Therefore, the magnetic field on the CV parent body was not a localized event like that expected for a field generated by meteoroid impact plasmas and instead likely had a wide spatial extent. Further, given the younger I-Xe ages for Kaba compared to Allende (9-10 Ma and 2-3 Ma after Stillwater respectively), CV parent body magnetic fields existed over an extended period of time. Together, these data present compelling evidence for the presence of a core-generated dynamo on the CV chondrite parent body, persistent for at least 8 Ma.

  16. Chemical and physical studies of type 3 chondrites 12: The metamorphic history of CV chondrites and their components

    NASA Technical Reports Server (NTRS)

    Guimon, R. Kyle; Symes, Steven J. K.; Sears, Derek W. G.

    1995-01-01

    The induced thermoluminescence (TL) properties of 16 CV and CV-related chondrites, four CK chondrites and Renazzo (CR2) have been measured in order to investigate their metamorphic history. The petrographic, mineralogical and bulk compositional differences among the CV chondrites indicate that the TL sensitivity of the approximately 130 C TL peak is reflecting the abundance of ordered feldspar, especially in chondrule mesostasis, which in turn reflects parent-body metamorphism. The TL properties of 18 samples of homogenized Allende powder heated at a variety of times and temperatures, and cathodoluminescence mosaics of Axtell and Coolidge, showed results consistent with this conclusion. Five refractory inclusions from Allende, and separates from those inclusions, were also examined and yielded trends reflecting variations in mineralogy indicative of high peak temperatures (either metamorphic or igneous) and fairly rapid cooling. The CK chondrites are unique among metamorphosed chondrites in showing no detectable induced TL, which is consistent with literature data that suggests very unusual feldspar in these meteorites. Using TL sensitivity and several mineral systems and allowing for the differences in the oxidized and reduced subgroups, the CV and CV-related meteorites can be divided into petrologic types analogous to those of the ordinary and CO type 3 chondrites. Axtell, Kaba, Leoville, Bali, Arch and ALHA81003 are type 3.0-3.1, while ALH84018, Efremovka, Grosnaja, Allende and Vigarano are type 3.2-3.3 and Coolidge and Loongana 001 are type 3.8. Mokoia is probably a breccia with regions ranging in petrologic type from 3.0 to 3.2. Renazzo often plots at the end of the reduced and oxidized CV chondrite trends, even when those trends diverge, suggesting that in many respects it resembles the unmetamorphosed precursors of the CV chondrites. The low-petrographic types and low-TL peak temperatures of all samples, including the CV3.8 chondrites, indicates metamorphism in the stability field of low feldspar (i.e., less than 800 C) and a metamorphic history similar to that of the CO chondrites but unlike that of the ordinary chondrites.

  17. Sonication inhibited browning but decreased polyphenols contents and antioxidant activity of fresh apple (malus pumila mill, cv. Red Fuji) juice.

    PubMed

    Sun, Yujing; Zhong, Liezhou; Cao, Lianfei; Lin, Wenwen; Ye, Xingqian

    2015-12-01

    Enzyme browning is the main challenge in the preparation of fresh apple juice. The influence of sonication on browning, as well as polyphenols and antioxidant activity of fresh apple juice was investigated. It was found that ultrasound can inhibit the browning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice, but decreased the contents of total phenolic content (TPC), total flavonoid content (TFC) and chlorogenic acid and reduced the antioxidant activity. On the whole, ultrasound technology cannot be used to the antibrowning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice. PMID:26604412

  18. The digestion of fresh perennial ryegrass (Lolium perenne L. cv. Melle) and white clover (Trifolium repens L. cv. Blanca) by growing cattle fed indoors.

    PubMed

    Beever, D E; Thomson, D J; Ulyatt, M J; Cammell, S B; Spooner, M C

    1985-11-01

    1. Pure swards of perennial ryegrass (Lolium perenne L. cv. Melle) or white clover (Trifolium repens L. cv. Blanca) were harvested daily at three and two stages of growth respectively, and offered to housed cattle. The grass diets comprised primary growth (May) and two later regrowths of contrasting morphology (i.e. leaf: stem values of 1.54 and 2.84 respectively), and were characterized by high contents of water-soluble carbohydrate and neutral-detergent fibre and comparable in vitro dry matter (DM) digestibilities (mean 0.80). Total nitrogen content was higher on primary growth grass (34 g/kg DM) than on regrowths (23 g/kg DM) but lower than values obtained for the two clover diets (38 and 43 g/kg DM, respectively). The clover diets had lower water-soluble carbohydrate contents than the grasses, comparable cellulose, but lower neutral-detergent fibre contents and in vitro DM digestibilities of 0.70 and 0.77 respectively. 2. The experiment lasted from May until August, during which time a total of twenty-one young Friesian steers (initial average live weight 130 kg) were used to determine both nutrient supply to the small intestine (twelve animals) and apparent digestibility (nine animals). Each diet was offered at three levels of DM intake (i.e. 18, 22 and 26 g/kg live weight). A further six steers, all fed at the rate of 22 g DM/kg live weight, were used to determine the metabolizable energy contents of the five diets by means of open-circuit calorimetry. 3. The three grass diets and the later-cut clover had, as intended, quite similar in vivo organic matter digestibilities, but that of the earlier-cut clover was lower, and this was associated with a large number of flower heads in this crop at the time of feeding. 4. On the clover diets, proportionately less of the ingested organic matter appeared to be digested in the rumen (0.40) compared with the grass diets (0.58) (P less than 0.001). On the high-N primary grass and the clover diets, substantial rumen losses of N were detected (P less than 0.01) compared with regrowth grasses. 5. The metabolizable energy content of the primary growth of grass was 12.2 MJ/kg DM, whilst the values for the other two grass diets were lower (11.6 MJ/kg DM), despite no marked decline in overall energy digestibility. Values for the two clover diets (mean 10.5 MJ/kg DM) were considerably lower than all values noted for the grasses.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:3870696

  19. The efficiency of energy utilization in growing cattle consuming fresh perennial ryegrass (Lolium perenne cv. Melle) or white clover (Trifolium repens cv. Blanca).

    PubMed

    Cammell, S B; Thomson, D J; Beever, D E; Haines, M J; Dhanoa, M S; Spooner, M C

    1986-05-01

    1. Twenty Friesian steers (225 kg live weight) were fed on mid- (M) (June-July) and late- (L) (August-September) season crops of either fresh perennial ryegrass (Lolium perenne cv. Melle) (G) or white clover (Trifolium repens cv. Blanca) (C). Each of the forage diets was offered at three restricted planes of nutrition above maintenance to compare the effect of forage species on the efficiency of energy utilization. All diets were harvested daily from swards of regrowth forage of intended equivalent digestibility. 2. Faecal and urine excretions were measured for 7 and 5 d respectively, followed by two consecutive 24 h measurements of methane, carbon dioxide and oxygen exchange in open-circuit respiration chambers. 3. The apparent digestibility of the energy in perennial ryegrass (0.759) was marginally higher (P less than 0.01) than that in white clover (0.748); the mid- and late-season forages were of similar (P greater than 0.05) digestibilities. Metabolizable energy (ME): digestible energy (DE) in diet G (0.837) was significantly (P less than 0.001) different from that in diet C (0.812). The partition of energy losses when expressed as MJ/GJ gross energy intake (GEI) indicated that energy lost as methane was not significantly different (P greater than 0.05) either between forages (G 62.8, C 63.4) or between seasons (M 63.2, L 63.1). Energy excretion in urine was higher for cattle fed on diet C (77.5) compared with diet G (60.5) (P less than 0.001). Heat production was similar (P greater than 0.05) between forages (G 480, C 478), but lower (P less than 0.01) for L (471) compared with M(486). Energy retention (by difference) was lower (P less than 0.001) for diet C (132) than G (156) and for M (138) than L (149) (P less than 0.05). 4. Parallel-line analysis of unscaled ME intake (MEI) in relation to retained energy (RE; MJ/d) indicated that the efficiency of utilization (kt) was similar (P greater than 0.05) between perennial ryegrass (0.42) and white clover (0.46). Linear extrapolation of the values to zero energy retention indicated that maintenance requirements of ME (Em; MJ/d) were 23.3 for diet G and 28.8 for diet C. The extrapolated Em when expressed in relation to a measured fasting heat production (FHP) of 22.8 (MJ/d) resulted in a derived efficiency of utilization of ME for maintenance (km) of 0.97 (G) and 0.79 (C), suggesting an underestimate of Em for diet G.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:3676184

  20. Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”

    PubMed Central

    Sun, Jian; Wang, Hexiang; Ng, Tzi Bun

    2010-01-01

    A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White Cloud Bean”. Both trypsin inhibitors exhibited a molecular mass of 16?kDa and reduced the activity of trypsin with an IC50 value of about 0.6??M. Dithiothreitol attenuated the trypsin inhibitory activity, signifying that an intact disulfide bond is indispensable to the activity. [Methyl-3H] thymidine incorporation by leukemia L1210 cells was inhibited with an IC50 value of 28.8??M and 21.5??M, respectively. They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100??M. PMID:20617140

  1. Proliferation potential of 18-month-old callus of Ananas comosus L. cv. Moris.

    PubMed

    De Silva, A E; Kadir, M A; Aziz, M A; Kadzimin, S

    2006-01-01

    Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of Ananas comosus L. cv. Moris were assessed in vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS) media, 32.22 microM alpha-naphthaleneacetic acid (NAA) gave the highest mean fresh weight of callus (46.817 g). Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) was inferior to NAA, while b-naphthoxy acetic acid (BNOA) and p-chlorophenoxy acetic acid (4-CPA) were not effective in proliferating 18-months old callus. Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus. However, in terms of callus production, NAA at 32.22 microM was economically better. PMID:16493521

  2. The fractionation of noble gases in diamonds of CV3 Efremovka chondrite

    NASA Technical Reports Server (NTRS)

    Fisenko, A. V.; Verchovsky, A. B.; Semjonova, L. F.; Shukolyukov, Yu. A.

    1993-01-01

    It was shown that in diamonds of Efremovka CV3 the noble gases with normal isotopic compositions are fractionated in different degree while the correlation of isotopic anomalous components is nearly constant. Some data for noble gases in DE-4 sample of Efremovka chondrite are considered. In contrast to DE-2 sample the DE-4 was treated except conc. HClO4, 220 C in addition with mixture of conc. H2SO4+H3PO4 (1:1), 220 C, twice. Noble gases analysis were performed in Germany at Max Plank Institute fur Chemie. Noble gases were released by oxidation of samples at stepped heating from 420 C to 810 C and by pyrolysis at 580, 590, and 680 C.

  3. Persimmon cv. Hachiya (Diospyros kaki Thunb.) fruit: some physical, chemical and nutritional properties.

    PubMed

    Celik, Ahmet; Ercisli, Sezai

    2008-01-01

    The persimmon cv. Hachiya (Diospyros kaki) fruits were analysed for some physical properties (fruit dimensions, fruit mass, fruit volume, fruit density, aspect ratio, geometric mean diameter, sphericity, spread area, projected area, bulk density, skin and flesh firmness, skin and flesh colour as L, a and b values, coefficient of static friction on different surfaces and porosity), chemical properties (moisture, ash, pH, acidity, vitamin C, total soluble solids) and nutritional properties (phosphorus, potassium, calcium, magnesium, sodium, iron, manganese, copper and zinc). The fruit characteristics ranged from 169 g for average fruit mass, 65.97 mm for the geometric mean diameter, 1.03% for sphericity and 180 cm3 for volume of fruit. The bulk density, fruit density and porosity were determined as 5,817 N/m3, 9,300 N/m3 and 38.06%, respectively. The present study also revealed important nutritional values of persimmon fruits. PMID:19382348

  4. Contrasting Size Distributions of Chondrules and Inclusions in Allende CV3

    NASA Technical Reports Server (NTRS)

    Fisher, Kent R.; Tait, Alastair W.; Simon, Jusin I.; Cuzzi, Jeff N.

    2014-01-01

    There are several leading theories on the processes that led to the formation of chondrites, e.g., sorting by mass, by X-winds, turbulent concentration, and by photophoresis. The juxtaposition of refractory inclusions (CAIs) and less refractory chondrules is central to these theories and there is much to be learned from their relative size distributions. There have been a number of studies into size distributions of particles in chondrites but only on relatively small scales primarily for chondrules, and rarely for both Calcium Aluminum-rich Inclusions (CAIs) and chondrules in the same sample. We have implemented macro-scale (25 cm diameter sample) and high-resolution microscale sampling of the Allende CV3 chondrite to create a complete data set of size frequencies for CAIs and chondrules.

  5. Overseas trip report, CV 990 underflight mission. [Norwegian Sea, Greenland ice sheet, and Alaska

    NASA Technical Reports Server (NTRS)

    Gloersen, P.; Crawford, J.; Hardis, L.

    1980-01-01

    The scanning microwave radiometer-7 simulator, the ocean temperature scanner, and an imaging scatterometer/altimeter operating at 14 GHz were carried onboard the NASA CV-990 over open oceans, sea ice, and continental ice sheets to gather surface truth information. Data flights were conducted over the Norwegian Sea to map the ocean polar front south and west of Bear Island and to transect several Nimbus-7 footprints in a rectangular pattern parallel to the northern shoreline of Norway. Additional flights were conducted to obtain correlative data on the cryosphere parameters and characteristics of the Greenland ice sheet, and study the frozen lakes near Barrow. The weather conditions and flight path way points for each of the nineteen flights are presented in tables and maps.

  6. Defect Detection in Superconducting Radiofrequency Cavity Surface Using C + + and OpenCV

    NASA Astrophysics Data System (ADS)

    Oswald, Samantha; Thomas Jefferson National Accelerator Facility Collaboration

    2014-03-01

    Thomas Jefferson National Accelerator Facility (TJNAF) uses superconducting radiofrequency (SRF) cavities to accelerate an electron beam. If theses cavities have a small particle or defect, it can degrade the performance of the cavity. The problem at hand is inspecting the cavity for defects, little bubbles of niobium on the surface of the cavity. Thousands of pictures have to be taken of a single cavity and then looked through to see how many defects were found. A C + + program with Open Source Computer Vision (OpenCV) was constructed to reduce the number of hours searching through the images and finds all the defects. Using this code, the SRF group is now able to use the code to identify defects in on-going tests of SRF cavities. Real time detection is the next step so that instead of taking pictures when looking at the cavity, the camera will detect all the defects.

  7. Petrography and classification of refractory inclusions in the Allende and Mokoia CV3 chondrites

    NASA Technical Reports Server (NTRS)

    Kornacki, A. S.; Cohen, R. E.; Wood, J. A.

    1983-01-01

    Results are reported for a comprehensive petrographic survey of the refractory inclusions in the Allende and Mokoia CV3 chondrites. More than 600 refractory inclusions in 22 thin sections of the meteorites were studied by optical and scanning-electron microscopy. Olivine-rich inclusions and Ca, Al-rich inclusions (CAIs) are aggregates of various combinations of three fundamental petrographic constituents: rimmed concentric objects, Ca, Si-rich chaotic material, and mafic inclusion matrix. A new classification system for refractory inclusions is developed that is based on the size and abundance of these three fundamental constituents. The new classification system avoids several problems that are inherent in other classification systems, which use the term 'coarse-grained' too restrictively for many simple CAIs and inaccurately for most mililite-rich complex CAIs.

  8. Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv. 'Tropic Sun')

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydr...

  9. Water Deficit Effect on Ratio of Seed to Berry Fresh Weight and Berry Weight Uniformity in Winegrape cv. Merlot

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Field-grown grapevines cv. Merlot (Vitis vinifera L.) were differentially irrigated in a randomized block design during two growing seasons to maintain a high or low level of vine water stress between fruit set and harvest. Detached berries from clusters harvested at maturity were individually weig...

  10. Confirmation By QTL mapping Of The Malus Robusta (Cv. Robusta 5) derived powdery mildew resistance gene Pl1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Geneva® apple rootstock breeding program has made extensive use of Malus robusta cv. Robusta 5 as a source of resistance to fire blight. Robusta 5 has also been used as the source of powdery mildew resistance by other breeding programs and a single locus Pl1 has been associated with this resist...

  11. Michael W. Cole (mwcole@mwcole.net) CV March 11, 2015 1 Michael W. Cole 197 University Ave.

    E-print Network

    Cole, Michael W.

    Michael W. Cole (mwcole@mwcole.net) ­ CV ­ March 11, 2015 1 Michael W. Cole 197 University Ave. Newark, NJ 07102 Phone: (314) 632-6536 E-mail: mwcole@mwcole.net Web: www.colelab.org Education 2009, with the interdisciplinary backgrounds, deep knowledge in chosen disciplines, and technical, professional, and personal

  12. Inherited transgene expression of the uidA and bar genes in Lilium longiflorum cv. Nellie White

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The expression of two transgenes, bar and uidA, was studied in Lilium longiflorum cv. Nellie White plants. ‘Nellie White’ had been transformed using the gene gun to bombard with pDM327 that contains the bar-uidA fusion gene under control of the CaMV 35S promoter. PCR analysis confirmed that eight ...

  13. High Efficiency Somatic Embrogenesis and Plant Regeneration in Suspension Cultures of an Ornamental Ginger Hybrid (Hedychium muluense x cv ‘Starburst’)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plants were successfully regenerated via somatic embryogenesis from shoot apex-derived callus of an ornamental ginger hybrid, Hedychium muluense x cv ‘Starburst’. H. muluense is a dwarf species and ‘Starburst’ is a hybrid cultivar with white and very fragrant flowers in a circular, wheel-like arrang...

  14. Abstract Embryogenic cultures of soybean [Glycine max (L.) Merr. cv. Jack and Asgrow A2872] were established in

    E-print Network

    Parrott, Wayne

    Abstract Embryogenic cultures of soybean [Glycine max (L.) Merr. cv. Jack and Asgrow A2872] were has been effective for the transformation of soybean [Glycine max (L.) Merr.] using microprojectile of soybean embryogenic cultures within their normal 2-week subculture period, and to determine if differences

  15. First report of downy mildew caused by Plasmopara halstedii on black-eyed susan (Rudbeckia fulgida cv. ‘Goldsturm’) in Maryland

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The North American perennial black-eyed Susan (Rudbeckia fulgida cv. ‘Goldsturm’) is an important nursery crop, prized by gardeners and landscapers for its persistent bloom and ease of cultivation. In September 2013 disease symptoms characteristic of downy mildew were observed from multiple plants a...

  16. Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg

    E-print Network

    Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg GRECO Cvo UNI OL/ Nds.PV-Symp. 06-2009 semiconductor physics / quantum solar energy conversion energy and semiconductor research (EHF) amongst regular lectures Educational Programs (PPRE, EUREC) #12

  17. Wintercuring of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid in response to Xylella fastidiosa infections

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Clonal replicates of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid P 63-61 were inoculated with Xylella fastidiosa strain M23 and evaluated for Almond Leaf Scorch Disease and subsequent wintercuring of infections during three growing seasons. Initial inoculations established gr...

  18. The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface*

    PubMed Central

    Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N.; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A.; Bianchet, Mario A.; Wang, Lai-Xi; Wilson, Iain B. H.; Vasta, Gerardo R.

    2013-01-01

    The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is “hijacked” by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288,) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 “self”-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

  19. Abundance, major element composition and size of components and matrix in CV, CO and Acfer 094 chondrites

    NASA Astrophysics Data System (ADS)

    Ebel, Denton S.; Brunner, Chelsea; Konrad, Kevin; Leftwich, Kristin; Erb, Isabelle; Lu, Muzhou; Rodriguez, Hugo; Crapster-Pregont, Ellen J.; Friedrich, Jon M.; Weisberg, Michael K.

    2016-01-01

    The relative abundances and chemical compositions of the macroscopic components or "inclusions" (chondrules and refractory inclusions) and fine-grained mineral matrix in chondritic meteorites provide constraints on astrophysical theories of inclusion formation and chondrite accretion. We present new techniques for analysis of low count/pixel Si, Mg, Ca, Al, Ti and Fe X-ray intensity maps of rock sections, and apply them to large areas of CO and CV chondrites, and the ungrouped Acfer 094 chondrite. For many thousands of manually segmented and type-identified inclusions, we are able to assess, pixel-by-pixel, the major element content of each inclusion. We quantify the total fraction of refractory elements accounted for by various types of inclusion and matrix. Among CO chondrites, both matrix and inclusion Mg/Si ratios approach the solar (and bulk CO) ratio with increasing petrologic grade, but Si remains enriched in inclusions relative to matrix. The oxidized CV chondrites with higher matrix/inclusion ratios exhibit more severe aqueous alteration (oxidation), and their excess matrix accounts for their higher porosity relative to reduced CV chondrites. Porosity could accommodate an original ice component of matrix as the direct cause of local alteration of oxidized CV chondrites. We confirm that major element abundances among inclusions differ greatly, across a wide range of CO and CV chondrites. These abundances in all cases add up to near-chondritic (solar) bulk abundance ratios in these chondrites, despite wide variations in matrix/inclusion ratios and inclusion sizes: chondrite components are complementary. This complementarity provides a robust meteoritic constraint for astrophysical disk models.

  20. CvADH1, a member of short-chain alcohol dehydrogenase family, is inducible by gibberellin and sucrose in developing watermelon seeds.

    PubMed

    Kim, Joonyul; Kang, Hong-Gyu; Jun, Sung-Hoon; Lee, Jinwon; Yim, Jieun; An, Gynheung

    2003-01-01

    To understand the molecular mechanisms that control seed formation, we selected a seed-preferential gene (CvADH1) from the ESTs of developing watermelon seeds. RNA blot analysis and in situ localization showed that CvADH1 was preferentially expressed in the nucellar tissue. The CvADH1 protein shared about 50% homology with short-chain alcohol dehydrogenase including ABA2 in Arabidopsis thaliana, stem secoisolariciresinol dehydrogenase in Forsythia intermedia, and 3beta-hydroxysterol dehydrogenase in Digitalis lanata. We investigated gene-expression levels in seeds from both normally pollinated fruits and those made parthenocarpic via N-(2-chloro-4-pyridyl)-N'-phenylurea treatment, the latter of which lack zygotic tissues. Whereas the transcripts of CvADH1 rapidly started to accumulate from about the pre-heart stage in normal seeds, they were not detectable in the parthenocarpic seeds. Treating the parthenogenic fruit with GA(3) strongly induced gene expression, up to the level accumulated in pollinated seeds. These results suggest that the CvADH1 gene is induced in maternal tissues by signals made in the zygotic tissues, and that gibberellin might be one of those signals. We also observed that CvADH1 expression was induced by sucrose in the parthenocarpic seeds. Therefore, we propose that the CvADH1 gene is inducible by gibberellin, and that sucrose plays an important role in the maternal tissues of watermelon during early seed development. PMID:12552151

  1. Unusual Dark Clasts in the Vigarano CV3 Chondrite: Record of Parent Body Process

    NASA Astrophysics Data System (ADS)

    Kojima, T.; Tomeoka, K.; Takeda, H.

    1993-07-01

    A variety of dark lithic clasts have been reported from CV3 chondrites and are commonly called "dark inclusions" (DIs). The DIs widely range in texture from chondritic with chondrules and Ca-Al-rich inclusions (CAIs) embedded in a matrix (similar to host meteorites), to fine-grained aggregates of Fe-rich olivine free of coarse-grained components [1,2]. The DIs have been interpreted to represent (1) primary aggregates of materials in the solar nebula [3-5] and (2) materials that were affected by thermal metamorphism on their parent bodies [6]. We present the results of petrographic and scanning electron microscope (SEM) studies of two unusual clasts found in the Vigarano CV3 chondrite. The two unusual clasts, which we will call CL1 and CL2, are approximately 1.2 x 1.0 mm^2 (CLl) and 0.8 x 0.6 mm^2 (CL2) and occur within one of the large clasts (2.8 x 1.0 mm^2). CL1 and CL2 have very similar mineralogies and textures; they contain irregular to oval-shaped inclusions consisting mostly of fine grains of Fe-rich olivine embedded in the matrix of the clasts, and are free of distinct chondrules, CAIs, and coarse mineral fragments. Thus, they resemble the fine-grained variety of DIs. Under the optical microscope, most inclusions resemble chondrules or chondrule fragments in shape and size. However, they are brownish-translucent in transmitted light and are clearly distinct from chondrules in the Vigarano host. The inclusions are characteristically flattened in direction, exhibiting apparent foliation. Our SEM observations reveal the following unusual characteristics: (1) the inclusions are not mere random aggregates of olivine grains but have peculiar internal textures, i.e., assemblies of round or oval-shaped outlines, which are suggestive of pseudomorphs after porphyritic or granular olivine chondrules; (2) one of the thick inclusion rims contains a network of vein-like strings of elongated olivine grains, (3) an Fe-Ni metal aggregate in CL1 has an Fe-, Ni-, S-rich halo, suggesting a reaction between its precursor and the surrounding matrix; and (4) olivine in the clasts commonly shows a swirly, fibrous texture similar to that of phyllosilicate. These characteristics are not reconciled with a primary (unprocessed) origin for the clasts but suggest that they were involved in a secondary process such as aqueous alteration. The fine grains of olivine in these clasts were presumably produced by thermal transformation of phyllosilicate. Serpentine is known to start transforming to olivine at >300 degrees C [7]. The inclusions in CL1 and CL2 resemble chondrules: Some of them are surrounded by distinctive rims similar to chondrule rims commonly seen in the carbonaceous chondrites. Therefore, we believe that the precursor material of the clasts would have been related to some types of chondrites, possibly the Vigarano host itself, and that aqueous alteration and thermal metamorphism occurred in the meteorite parent body. We suggest that some of the dark inclusions and clasts previously reported from CV3 chondrites and other types of meteorites may have origins common to these clasts in Vigarano. References: [1] Fruland R. M. et al. (1978) Proc. LPSC 9th, 1305-1329. [2] Johnson C. A. et al. (1990) GCA, 54, 819-830. [3] Bischoff A. et al. (1988) LPSC XIX, 88-89. [4] Kurat G. (1989) Z. Naturforsch., 44a, 988-1004. [5] Palme H. (1989) Z. Naturforsch., 44a, 1005-1014. [6] Bunch T. E. and Chang S. (1983) LPSC XIV, 75-76. [7] Akai J. (1992) Proc. NIPR Symp. Antarct. Meteorites, 5, 120-135.

  2. Safety and immunogenicity of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Chilean children.

    PubMed

    Lagos, Rosanna E; Muñoz, Alma E; Levine, Myron M; Lepetic, Alejandro; François, Nancy; Yarzabal, Juan Pablo; Schuerman, Lode

    2011-05-01

    The safety and immunogenicity of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV, Synflorix™) were assessed in 240 healthy Chilean children randomized to receive 3 doses of PHiD-CV (PHiD-CV group) or hepatitis A vaccine (HAV control group) at 2-4-6 months of age. All were offered 1 HAV dose at 12 months (outside study). The PHiD-CV group received a second HAV dose at 18-21 months and PHiD-CV booster at 20-23 months. The HAV control group received 2 PHiD-CV catch-up doses at 18-21 and 20-23 months. Adverse events were recorded and pneumococcal antibody responses and opsonophagocytic activity (OPA) were measured. Both PHiD-CV vaccination schedules were well tolerated and immunogenic against the pneumococcal vaccine serotypes and protein D. The reactogenicity of PHiD-CV primary, booster and catch-up doses was in line with previous PHiD-CV studies, although generally higher than with HAV. For each vaccine serotype, the percentage of subjects with antibody concentrations ?0.2 µg/ml (GSK's 22F-inhibition ELISA) was at least 93.2% following 3 PHiD-CV primary doses and at least 97.4% post-booster; percentages with OPA titers ?8 were at least 91.7% post-booster. After 2-dose catch-up, at least 94.3% of children had antibody concentrations ?0.2 µg/ml against each serotype except 6B (84.3%); at least 95.2% had OPA titers ?8 except against serotypes 1, 5 and 6B. In conclusion, the safety profiles of 2 PHiD-CV vaccination schedules (3-dose primary plus booster and 2-dose catch-up) were in line with previous studies and PHiD-CV was immunogenic for all 10 vaccine serotypes and protein D. PMID:21441782

  3. Chemical and spectroscopic characteristics of the wood of Vitis vinifera cv. Sangiovese affected by esca disease.

    PubMed

    Agrelli, Diana; Amalfitano, Carmine; Conte, Pellegrino; Mugnai, Laura

    2009-12-23

    Chemical and spectroscopic analyses ((13)C cross-polarization-magic angle spinning NMR and attenuated total reflection Fourier transform infrared spectroscopies) were carried out on the wood of Vitis vinifera cv. Sangiovese with brown-red discoloration and black streaks caused by esca disease. The analyses of the brown-red wood revealed the destruction of hemicelluloses and noncrystalline cellulose as well as modifications in the pectic and ligninic wood fractions. The pectic fraction consisted of carbohydrates associated with polyphenols. The lignin fraction exhibited only a few changes in the aromatic systems and a partial demethylation, and it appeared to be associated with condensed phenolic components probably arising from response polyphenols. The degradation of hemicelluloses and noncrystalline cellulose in brown-red wood, where the pathogens Phaeoacremonium aleophilum and Phaeomoniella chlamydospora prevail with respect to the other fungus Fomitiporia mediterranea, was consistent with reports on the degradative activity of such fungi in vitro carried out on model substrates. The observed alterations could also be attributed to the radical oxidation process caused by the oxidative response of defense itself triggered by infection, as suggested by the accumulation of postinfectional compounds. The analyses of wood tissue with black streaks showed less marked deterioration; here, an increase in pectic and phenolic substances, which probably accumulate in the xylem vessels as a response to the infection, was observed. PMID:19919033

  4. Irrigation effects on quality, phenolic composition, and selected volatiles of virgin olive oils cv. Leccino.

    PubMed

    Servili, Maurizio; Esposto, Sonia; Lodolini, Enrico; Selvaggini, Roberto; Taticchi, Agnese; Urbani, Stefania; Montedoro, Gianfrancesco; Serravalle, Matteo; Gucci, Riccardo

    2007-08-01

    Field-grown olive trees (Olea europaea L. cv. Leccino) were used over two growing seasons to determine the effect of deficit irrigation regimes on virgin olive oil (VOO) quality. Drip irrigation was managed to maintain a predawn leaf water potential (PLWP): (a) higher than -1.1 MPa (full irrigation: FI); (b) between -1.0 and -3.3 MPa (deficit irrigation: DI); (c) higher than -4.2 MPa (severe deficit irrigation: SI). The fruit yield and oil yield of DI trees were over 90% of those of FI treatments in both years, respectively, whereas yields of SI trees ranged from 61 to 76%. The irrigation regime had minor effects on the free acidity, peroxide value, and fatty acid composition of VOO. The concentrations of phenols and o-diphenols in VOO were negatively correlated with PLWP. The concentrations of the dialdehydic form of decarboxymethyl elenolic acid linked to (3,4-dihydroxyphenyl)ethanol (3,4-DHPEA-EDA), the isomer of the oleuropein aglycon (3,4-DHPEA-EA), and the dialdehydic form of decarboxymethyl elenolic acid linked to (p-hydroxyphenyl)ethanol (p-HPEA-EDA) were lower in FI than in SI treatments. The concentrations of lignans (+)-1-acetoxipinoresinol and (+)-1-pinoresinol were unaffected by the irrigation regime. The tree water status had a marked effect on the concentration of volatile compounds, such as the C(6)-saturated and unsaturated aldehydes, alcohols, and esters. PMID:17636938

  5. Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel.

    PubMed

    Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2012-01-01

    Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (-2.66, 62.66 mg/mL), Arabic gum (-1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. PMID:22489134

  6. Biomonitoring of air pollution with mercury in Croatia by using moss species and CV-AAS.

    PubMed

    Spiri?, Zdravko; Vu?kovi?, Ivana; Stafilov, Traj?e; Kušan, Vladimir; Ba?eva, Katerina

    2014-07-01

    Moss samples from four dominant species (Hypnum cupressiforme, Pleurozium schreberi, Homalothecium sericeum and Brachythecium rutabulum) were collected during the summer and autumn of 2010 from 121 sampling sites evenly distributed over the territory of Croatia. Samples were totally digested by using microwave digestion system, whilst mercury was analysed by using cold vapour atomic absorption spectrometry (CV-AAS). Descriptive statistics were done from analyses of mercury in all moss samples. The content of mercury ranged from 0.010 to 0.145 mg kg(-1) with a median value of 0.043 mg kg(-1). Hg distribution map shows the sites of the country with higher levels of this element. High contents of Hg were found in moss samples collected from the regions of Podravina and Istria as a result of anthropogenic pollution. Comparison of median values and ranges with those found in moss samples in 2006 shows slight reduction of mercury air pollution. When compared to the results obtained from recent studies conducted in Slovenia, Macedonia and especially in Norway-which serves as a reference considering the fact that it is a pristine area-mercury air pollution in Croatia is insignificant. PMID:24733436

  7. Transfer cell wall ingrowths and transport capacity in pea leaf discs. [Pisum sativum cv

    SciTech Connect

    Wimmers, L.E.; Turgeon, R.

    1986-04-01

    Transfer cell wall ingrowths are thought to increase transport capacity by increasing plasmelemma surface area. Little direct evidence for this hypothesis exists since experimental systems in which the surface area of wall ingrowths can be modulated have not been available. They grew Pisum sativum cv. Little Marvel plants under three light regimes (150, 500, 1000 umol photons m/sup -2/ sec/sup -1/) using 1000 watt Sylvania Metal Halide lamps. Wall ingrowths in minor vein phloem parenchyma cells were analyzed morphometrically from electron micrographs and a positive correlation was found between light intensity and extent of wall ingrowths. Vein loading was assayed by floating abraded leaf discs on /sup 14/C-sucrose (1 mM). There was a positive correlation between uptake and transfer cell wall surface area, although the latter increased more than the former. No significant differences were found in vein length, numbers of phloem elements, or phloem cross sectional areas. Changes in light intensity after a leaf reached maturity did not change uptake potential over a period of at least three days.

  8. Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. Cv. MRQ 74)

    PubMed Central

    Taha, Rosna Mat; Saleh, Azani; Mahmad, Noraini; Hasbullah, Nor Azlina; Mohajer, Sadegh

    2012-01-01

    Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5?mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5?mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1?mg/L BAP, and 0.1?mg/L ?-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1?mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds. PMID:22919338

  9. Sequence analysis of ORF IV RTBV isolated from tungro infected Oryza sativa L. cv Ciherang

    NASA Astrophysics Data System (ADS)

    Hastilestari, Bernadetta Rina; Astuti, Dwi; Estiati, Amy; Nugroho, Satya

    2015-09-01

    The Effort to increase rice production is often constrained by pest and disease such as Tungro. The Tungro disease is caused by the joint infection with two dissimilar viruses; a bacil-form-DNA virus, the Rice tungro bacilliform virus(RTBV) and the spherical RNA virus, Rice tungro spherical virus (RTSV) and transmitted by Green leafhopper (Nephotettix virescens). The symptom of disease is caused by the presence of RTBV. The genome of RTBV consists of four Open reading frames (ORFs) which encode functional proteins. Of the four, ORF IV is unique because it exists only in RTBV. The most efficient method of generating disease resistance plants is to look for natural sources of resistance genes in wild or germplasm and then transfer the gene and the accompanying resistance in cultivated crop varieties. The aim of this study is, therefore, to isolate and analyze of 1170 bp gene of ORF 4 of Tungro virus isolated from an Indonesian rice cultivar, Ciherang (Oryza sativa L. cv Indica). DNA sequencing analysis using BLAST showed 94% similarity with the reference sequence gen bank Acc.M65026.1. The comparisons and mutation analysis of DNA sequences were discussed in this research.

  10. Acylated anthocyanins from sprouts of Raphanus sativus cv. Sango: isolation, structure elucidation and antioxidant activity.

    PubMed

    Matera, Riccardo; Gabbanini, Simone; Berretti, Serena; Amorati, Riccardo; De Nicola, Gina Rosalinda; Iori, Renato; Valgimigli, Luca

    2015-01-01

    Little is known on structure-activity relationships of antioxidant anthocyanins. Raphanus sativus cv Sango sprouts are among the richest sources (270 mg/100 g fresh weight). We isolated from sprouts' juice 9 acylated anthocyanins, including 4 new compounds. All comprise a cyanidin core bearing 3-4 glucose units, multiply acylated with malonic and phenolic acids (ferulic and sinapic). All compounds were equally effective in inhibiting the autoxidation of linoleic acid in aqueous micelles, with rate constant for trapping peroxyl radicals kinh=(3.8 ± 0.7) × 10(4)M(-1)s(-1) at 37 °C. In acetonitrile solution kinh varied with acylation: (0.9-2.1) × 10(5)M(-1)s(-1) at 30 °C. Each molecule trapped a number n of peroxyl radicals ranging from 4 to 7. Anthocyanins bearing sinapic acid were more effective than those bearing the ferulic moiety. Under identical settings, deacylated cyanin, ferulic and sinapic acids had kinh of 0.4 × 10(5), 0.3 × 10(5) and 1.6 × 10(5)M(-1)s(-1) respectively, with n ranging 2-3. Results show the major role of acylation on antioxidant performance. PMID:25053073

  11. Transpiration, crop coefficient and water use of Olive tree (cv. Cordovil) in Southern Portugal

    NASA Astrophysics Data System (ADS)

    Santos, F. L.

    2008-12-01

    Orchard olive transpiration, soil water status and stomatal response in relation to water deficit were investigated to clarify mechanisms of tree water uptake and stomatal control to improve the irrigation scheduling of low-density olive trees of cv. Cordovil grown in typical Mediterranean environment of Southern Portugal. Trees were subject to three irrigation treatments. Treatment A received 100% of crop evapotranspiration by a drip irrigation system, a sustained deficit (SDI)treatment B received 60% of crop evapotranspiration, a regulated deficit(RDI) irrigation treatment C received irrigation water before-flowering and just before pit-hardening, and a Dry-farming treatment. Tree and orchard transpiration and the dynamics of water uptake by roots were estimated from sap flow measurements and water balance technique. Stomatal conductance was modeled from local meteorological variables, measured sap flow and tree canopy variables. Higher than treatment A and B stomatal conductance and the high tree fruit production recommend treatment C as most suitable for scheduling irrigation of olive orchards in wet years of well distributed late summer rainfall. For drier years of no summer and early autumn rains that minimizes available water to extract by roots outside the wet bulb of drip irrigation and for the scarce readily available irrigation water years, as so often occurs in the region, the sustained deficit irrigation (SDI) regime seems a better option. Nonetheless, for years of limited available water resources that preclude sustained deficit irrigation, careful management of the proposed RDI could also allow for efficient use of irrigation water.

  12. The impact of high temperatures on Vitis vinifera cv. Semillon grapevine performance and berry ripening

    PubMed Central

    Greer, Dennis H.; Weedon, Mark M.

    2013-01-01

    The heat event that occurred in many parts of Australia in 2009 was the worst on record for the past decade, with air temperatures exceeding 40°C for 14 days. Our aim was to assess the impacts of this heat event on vine performance, including ripening, yield, and gas exchange of Vitis vinifera cv. Semillon grown in a Riverina vineyard. To assess the affect of high temperatures on Semillon grapevines, the vines were covered with a protective layer to reduce radiant heating and were compared with vines exposed to ambient conditions. The heat event had major effects on ripening; reducing the rate of ripening by 50% and delaying harvest ripeness and causing a high incidence of berry shrivel and sunburn. Yield was not affected. Photosynthesis was reduced 35% by the heat event while transpiration increased nearly threefold and was accounted for by increased stomatal conductance. The conclusion of this study was that heat events delayed ripening in Semillon berries and caused a significant reduction in berry quality. Strategies to minimize the radiant load during heat events are required and this study has confirmed a protective layer can reduce canopy temperatures and enhance berry quality. PMID:24348494

  13. Measurement of heme efflux and heme content in isolated developing chloroplasts. [Cucumis sativus, cv. Sumter

    SciTech Connect

    Thomas, J.; Weinstein, J.D. )

    1990-11-01

    Hemes destined for cytosolic hemoproteins must originate in one of the cellular compartments which have the capacity for heme synthesis, namely the chloroplast or the mitochondria. Since developing chloroplasts from greening cucumber (Cucumis sativus, cv. Sumter) cotyledons are known to contain complete heme and chlorophyll biosynthetic pathways, they were tested for their capacity export hemes. Picomole quantities of heme were measured by reconstitution of the heme with apo-peroxidase and subsequent determination of peroxidase activity. The assay method was sensitive (as little as 0.7 picomole of heme could be detected in a volume of 100 microliters) and was linear with heme concentration. When intact plastids were incubated with apo-peroxidase, a steady-state rate of efflux between 0.12 and 0.45 picomole heme/minute/milligram plastid protein was measured. The efflux rate was not due to plastid breakage and could be enhanced by incubating with the heme precursor, {delta}-aminolevulinic acid. Cold acetone extraction removed 47 {plus minus} 17 picomoles heme/milligram plastid protein from the total b-type heme pool in the chloroplasts (166 {plus minus} 9 picomoles heme/milligram protein, by acid-acetone extraction). The reconstitution technique provided a similar estimate of readily exchangeable heme in the plastid, 37 {plus minus} 8 picomoles heme/milligram protein (or 6 micromolar in the plastids). These values may be indicative of a free heme pool which exists in the chloroplast.

  14. G-quadruplex formation in double strand DNA probed by NMM and CV fluorescence

    PubMed Central

    Kreig, Alex; Calvert, Jacob; Sanoica, Janet; Cullum, Emily; Tipanna, Ramreddy; Myong, Sua

    2015-01-01

    G-quadruplexes (GQs) are alternative DNA secondary structures that can form throughout the human genome and control the replication and transcription of important regulatory genes. Here, we established an ensemble fluorescence assay by employing two GQ-interacting compounds, N-methyl mesoporphyrin IX (NMM) and Crystal Violet (CV). This enables quantitative measurement of the GQ folding propensity and conformation specificity in both single strand (ss) and double strand (ds) DNA. Our GQ mapping indicates that the likelihood of GQ formation is substantially diminished in dsDNA, likely due to the competition from the Watson–Crick base pairing. Unlike GQ folding sequence in ssDNA which forms both parallel and antiparallel GQs, dsDNA displays only parallel folding. Additionally, we employed single molecule FRET to obtain a direct quantitation of stably formed-, weakly folded and unfolded GQ conformations. The findings of this study and the method developed here will enable identifying and classifying potential GQ-forming sequences in human genome. PMID:26202971

  15. Impacts of strigolactone on shoot branching under phosphate starvation in chrysanthemum (Dendranthema grandiflorum cv. Jinba)

    PubMed Central

    Xi, Lin; Wen, Chao; Fang, Shuang; Chen, Xiaoli; Nie, Jing; Chu, JinFang; Yuan, Cunquan; Yan, Cunyu; Ma, Nan; Zhao, Liangjun

    2015-01-01

    Chrysanthemum (Dendranthema grandiflorum cv. Jinba) shoot branching is determined by bud outgrowth during the vegetative growth stage. The degree of axillary bud outgrowth is highly influenced by environmental conditions, such as nutrient availability. Here, we demonstrated that phosphorus (Pi) starvation significantly reduces axillary bud outgrowth in chrysanthemum. A strigolactone (SL) biosynthesis gene, DgCCD7, was isolated and characterized as an ortholog of MAX3/DAD3/RMS5/D17. By using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS), three putative SLs were identified and levels of all three SLs showed strong increase under Pi starvation conditions. Determinations of the distribution of SLs and regulation of DgCCD7/8 in response to Pi changes in root indicate that SL acts systemically. However, temporal expression patterns of biosynthesis and signaling genes in nodes revealed that Pi starvation causes a local response of SL pathway. Treatment of node segments with or without auxin and Pi revealed that in the absence of exogenous auxin, Pi delayed axillary buds outgrowth and up-regulated local SL pathway genes. These data indicated that an auxin-SL regulatory loop responded to Pi starvation for delaying bud outgrowth locally, root biosynthesized SLs were transported acropetally and functioned in shoot branching inhibition under Pi starvation. We proposed that SLs contributed to chrysanthemum shoot branching control in response to Pi-limiting conditions in a systemic way. PMID:26442011

  16. Characterization of Fusarium wilt resistant somaclonal variants of banana cv. Rasthali by cDNA-RAPD.

    PubMed

    Ghag, Siddhesh B; Shekhawat, Upendra K S; Ganapathi, Thumballi R

    2014-12-01

    Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is counted among the most destructive diseases of crop plants in India. In the absence of any credible control measure to manage this disease, development of resistant cultivars is the best option. Somaclonal variations arising out of long term in vitro culture of plant tissues is an important source of genetic variability and the selection of somaclones having desired characteristics is a promising strategy to develop plants with improved characters. In the present study, we isolated a group of somaclonal variants of banana cv. Rasthali which showed efficient resistance towards Foc race 1 infection in repeated bioassays. cDNA-RAPD methodology using 96 decamer primers was used to characterize these somaclonal variants. Among the four differentially amplified bands obtained, one mapping to the coding region of a lipoxygenase gene was confirmed to be down regulated in the somaclones as compared to controls by real-time quantitative RT-PCR. Our results correlated well with earlier studies with lipoxygenase mutants in maize wherein reduced expression of lipoxygenase led to enhanced resistance towards Fusarium infection. PMID:25160909

  17. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex.

    PubMed

    Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

    2013-03-01

    Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees' latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins' autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458

  18. [In vitro and in vivo effects of mango pulp (Mangifera indica cv. Azucar) in colon carcinogenesis].

    PubMed

    Corrales-Bernal, Andrea; Amparo Urango, Luz; Rojano, Benjamín; Maldonado, Maria Elena

    2014-03-01

    Mango pulp contains ascorbic acid, carotenoids, polyphenols, terpenoids and fiber which are healthy and could protect against colon cancer. The aim of this study was to evaluate the antiproliferative and preventive capacity of an aqueous extract of Mangifera indica cv. Azúcar on a human colon adenocarcinoma cell line (SW480) and in a rodent model of colorectal cancer, respectively. The content of total phenolics, flavonoids and carotenoids were also analyzed in the extract. SW480 cell growth was inhibited in a dose and time dependent manner by 22.3% after a 72h exposure to the extract (200 µg/ mL). Colon carcinogenesis was initiated in Balb/c mice by two intra-peritoneal injections of azoxymethane (AOM) at the third and fourth week of giving mango in drinking water (0.3%, 0.6%, 1.25%). After 10 weeks of treatment, in the colon of mice receiving 0.3% mango, aberrant crypt foci formation was inhibited more than 60% (p=0,05) and the inhibition was dose-dependent when compared with controls receiving water. These results show that mango pulp, a natural food, non toxic, part of human being diet, contains bioactive compounds able to reduce growth of tumor cells and to prevent the appearance of precancerous lesions in colon during carcinogenesis initiation. PMID:25796713

  19. G-quadruplex formation in double strand DNA probed by NMM and CV fluorescence.

    PubMed

    Kreig, Alex; Calvert, Jacob; Sanoica, Janet; Cullum, Emily; Tipanna, Ramreddy; Myong, Sua

    2015-09-18

    G-quadruplexes (GQs) are alternative DNA secondary structures that can form throughout the human genome and control the replication and transcription of important regulatory genes. Here, we established an ensemble fluorescence assay by employing two GQ-interacting compounds, N-methyl mesoporphyrin IX (NMM) and Crystal Violet (CV). This enables quantitative measurement of the GQ folding propensity and conformation specificity in both single strand (ss) and double strand (ds) DNA. Our GQ mapping indicates that the likelihood of GQ formation is substantially diminished in dsDNA, likely due to the competition from the Watson-Crick base pairing. Unlike GQ folding sequence in ssDNA which forms both parallel and antiparallel GQs, dsDNA displays only parallel folding. Additionally, we employed single molecule FRET to obtain a direct quantitation of stably formed-, weakly folded and unfolded GQ conformations. The findings of this study and the method developed here will enable identifying and classifying potential GQ-forming sequences in human genome. PMID:26202971

  20. Diurnal cycles of embolism formation and repair in petioles of grapevine (Vitis vinifera cv. Chasselas)

    PubMed Central

    Zufferey, V.; Cochard, H.; Ameglio, T.; Spring, J.-L.; Viret, O.

    2011-01-01

    The impact of water deficit on stomatal conductance (gs), petiole hydraulic conductance (Kpetiole), and vulnerability to cavitation (PLC, percentage loss of hydraulic conductivity) in leaf petioles has been observed on field-grown vines (Vitis vinifera L. cv. Chasselas). Petioles were highly vulnerable to cavitation, with a 50% loss of hydraulic conductivity at a stem xylem water potential (?x) of –0.95?MPa, and up to 90% loss of conductivity at a ?x of –1.5?MPa. Kpetiole described a daily cycle, decreasing during the day as water stress and evapotranspiration increased, then rising again in the early evening up to the previous morning's Kpetiole levels. In water-stressed vines, PLC increased sharply during the daytime and reached maximum values (70–90%) in the middle of the afternoon. Embolism repair occurred in petioles from the end of the day through the night. Indeed, PLC decreased in darkness in water-stressed vines. PLC variation in irrigated plants showed the same tendency, but with a smaller amplitude. The Chasselas cultivar appears to develop hydraulic segmentation, in which petiole cavitation plays an important role as a ‘hydraulic fuse’, thereby limiting leaf transpiration and the propagation of embolism and preserving the integrity of other organs (shoots and roots) during water stress. In the present study, progressive stomatal closure responded to a decrease in Kpetiole and an increase in cavitation events. Almost total closure of stomata (90%) was measured when PLC in petioles reached >90%. PMID:21447755

  1. Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.

    PubMed

    Kanamoto, Hirosuke; Yamashita, Atsushi; Asao, Hiroshi; Okumura, Satoru; Takase, Hisabumi; Hattori, Masahira; Yokota, Akiho; Tomizawa, Ken-Ichi

    2006-04-01

    Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to approximately 36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants. PMID:16604461

  2. Structural Insight into Cell Wall Architecture of Micanthus sinensis cv. using Correlative Microscopy Approaches.

    PubMed

    Ma, Jianfeng; Lv, Xunli; Yang, Shumin; Tian, Genlin; Liu, Xing'e

    2015-10-01

    Structural organization of the plant cell wall is a key parameter for understanding anisotropic plant growth and mechanical behavior. Four imaging platforms were used to investigate the cell wall architecture of Miscanthus sinensis cv. internode tissue. Using transmission electron microscopy with potassium permanganate, we found a great degree of inhomogeneity in the layering structure (4-9 layers) of the sclerenchymatic fiber (Sf). However, the xylem vessel showed a single layer. Atomic force microscopy images revealed that the cellulose microfibrils (Mfs) deposited in the primary wall of the protoxylem vessel (Pxv) were disordered, while the secondary wall was composed of Mfs oriented in parallel in the cross and longitudinal section. Furthermore, Raman spectroscopy images indicated no variation in the Mf orientation of Pxv and the Mfs in Pxv were oriented more perpendicular to the cell axis than that of Sfs. Based on the integrated results, we have proposed an architectural model of Pxv composed of two layers: an outermost primary wall composed of a meshwork of Mfs and inner secondary wall containing parallel Mfs. This proposed model will support future ultrastructural analysis of plant cell walls in heterogeneous tissues, an area of increasing scientific interest particularly for liquid biofuel processing. PMID:26358178

  3. CV-990 Landing Systems Research Aircraft (LSRA) flight #145 drilling of shuttle tire using Tire Assa

    NASA Technical Reports Server (NTRS)

    1995-01-01

    Created from a 1/16th model of a German World War II tank, the TAV (Tire Assault Vehicle) was an important safety feature for the Convair 990 Landing System Research Aircraft, which tested space shuttle tires. It was imperative to know the extreme conditions the shuttle tires could tolerate at landing without putting the shuttle and its crew at risk. In addition, the CV990 was able to land repeatedly to test the tires. The TAV was built from a kit and modified into a radio controlled, video-equipped machine to drill holes in aircraft test tires that were in imminent danger of exploding because of one or more conditions: high air pressure, high temperatures, and cord wear. An exploding test tire releases energy equivalent to two and one-half sticks of dynamite and can cause severe injuries to anyone within 50 ft. of the explosion, as well as ear injury - possibly permanent hearing loss - to anyone within 100 ft. The degree of danger is also determined by the temperature pressure and cord wear of a test tire. The TAV was developed by David Carrott, a PRC employee under contract to NASA.

  4. Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers

    SciTech Connect

    Wenling Hsieh; Sze, Heven )

    1991-05-01

    The transport properties of Ca-pumping ATPases from carrot (Daucus carota cv. Danvers) tissue culture cells were studied. ATP dependent Ca transport in vesicles that comigrated with an ER marker, was stimulated 3-4 fold by calmodulin. Cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum Ca-ATPase) partially inhibited oxalate-stimulated Ca transport activity; however, it had little or not effect on calmodulin-stimulated Ca uptake. The results suggested the presence of two types of Ca ATPases, and ER- and a plasma membrane-type. Incubation of membranes with (gamma{sup 32}P)ATP resulted in the formation of a single acyl ({sup 32}P) phosphoprotein of 120 kDa. Formation of this phosphoprotein was dependent on Ca, and enhanced by La {sup 3+}, characteristic of the plasma membrane CaATPase. Acidic phospholipids, like phosphatidylserine, stimulated Ca transport, similar to their effect on the erythrocyte plasma membrane CaATPase. These results would indicate that the calmodulin-stimulated Ca transport originated in large part from a plasma membrane-type Ca pump of 120 kDa.

  5. Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)

    PubMed Central

    Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng

    2009-01-01

    A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 ?M, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 ?M. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172

  6. Phenolics from strawberry cv. Falandi and their antioxidant and ?-glucosidase inhibitory activities.

    PubMed

    Yang, Dan; Xie, Haihui; Jiang, Yueming; Wei, Xiaoyi

    2016-03-01

    Three new phenolic glucosides, falandiosides A (1), B (2), and C (6) were isolated from strawberry (Fragaria×ananassa Duch.) cv. Falandi fruit, together with three flavone glucuronides (3-5), eleven lignan glycosides (12-22), and five others. Their structures were determined by spectroscopic methods. All the known phenolics were reported from strawberry for the first time. They were evaluated for antioxidant and ?-glucosidase inhibitory activities. Three new and fifteen known phenolics showed potent 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity with IC50 values of 22.50-4.28?M in comparison to l-ascorbic acid (14.21?M). Quercetin 3-(6-methylglucuronide) (4), (+)-isolariciresinol 9'-glucoside (12), and (-)-isolariciresinol 9'-glucoside (13) were active in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Moreover, compounds 12 and 13 had moderate ferric reducing antioxidant power (FRAP) values. Further, two new and seven known phenolics exhibited more potent ?-glucosidase inhibitory activity with IC50 values of 537.43-25.39?M than acarbose (619.94?M). PMID:26471628

  7. Hydrolysis of Conjugated Gibberellins by ?-Glucosidases from Dwarf Rice (Oryza sativa L. cv. «Tan-ginbozu»).

    PubMed

    Schliemann, W

    1984-09-01

    Dwarf rice (Oryza sativa L. cv. «Tan-ginbozu») is widely used for gibberellin conjugate bioassay. Soluble and particulate fractions from seeds and seedlings of this variety showed hydrolytic activity toward [(3)H]GA-O-Glc. The soluble fraction from mature seeds exhibited the highest [(3)H]GA-O-Glc/pNP-?-Glc hydrolysis ratio which decreased during the early germination phase. The soluble ?-glucosidases from mature seeds were efficiently separated by CMSephadex C-50 column chromatography. It was found that the most active ?-glucosidase CM 1 from seeds hydrolyses GA(8)-O-2-Glc 8 times faster than GA(3)-O-3-Glc and GA(3) Glc ester. In shoots of 4-d-old etiolated seedlings a soluble ?-glucosidase (CM 4) is present which showed a 200 times faster hydrolysis of GA(8)-O-2-Glc as compared to GA(3)-O-3-Glc. Both the ?-glucosidase CM 4 and the main ?-glucosidase component CM 5 possessed relatively high GA(3) Glc ester hydrolysing activity. Our enzymatic findings demonstrate that in dwarf rice bioassay (leaf application) the GA-OGlc with equatorially arranged glucosyl residues (O-2-glucosides of GA(8), GA(26), GA(27), and GA(29)) and GA Glc esters are hydrolysed by the concerted action of the endogenous a-glucosidases CM 4 and CM 5 from shoots. PMID:23195047

  8. The BANANA project. V. Misaligned and precessing stellar rotation axes in CV Velorum

    E-print Network

    Albrecht, Simon; Torres, Guillermo; Fabrycky, Daniel C; Setiawan, Johny; Gillon, Michael; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

    2014-01-01

    As part of the BANANA project (Binaries Are Not Always Neatly Aligned), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of $\\beta_{\\rm p} = -52\\pm6^{\\circ}$ and $\\beta_{\\rm s}= 3\\pm7^{\\circ}$ for the primary and secondary stars (B2.5V $+$ B2.5V, $P=6.9$ d). We combine this information with several measurements of changing projected stellar rotation speeds ($v \\sin i_{\\star}$) over the last $30$ years, leading to a model in which the primary star's obliquity is $\\approx65^{\\circ}$, and its spin axis precesses around the total angular momentum vector with a period of about $140$ years. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the $v \\sin i_{\\star}$. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronge...

  9. The BANANA Project. V. Misaligned and Precessing Stellar Rotation Axes in CV Velorum

    NASA Astrophysics Data System (ADS)

    Albrecht, Simon; Winn, Joshua N.; Torres, Guillermo; Fabrycky, Daniel C.; Setiawan, Johny; Gillon, Michaël; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

    2014-04-01

    As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of ?p = -52° ± 6° and ?s = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i sstarf) over the last 30 yr, leading to a model in which the primary star's obliquity is ?65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i sstarf. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary. Based on observations made with ESOs 2.2 m Telescopes at the La Silla Paranal Observatory under programme ID 084.C-1008 and under MPIA guaranteed time.

  10. The BANANA project. V. Misaligned and precessing stellar rotation axes in CV Velorum

    SciTech Connect

    Albrecht, Simon; Winn, Joshua N.; Triaud, Amaury; Torres, Guillermo; Fabrycky, Daniel C.; Setiawan, Johny; Gillon, Michaël; Jehin, Emmanuel; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

    2014-04-20

    As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of ?{sub p} = –52° ± 6° and ?{sub s} = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i {sub *}) over the last 30 yr, leading to a model in which the primary star's obliquity is ?65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i {sub *}. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary.

  11. Sink to Source Transition in Tendrils of a Semileafless Mutant, Pisum sativum cv Curly 1

    PubMed Central

    Côté, Richard; Gerrath, Jean M.; Peterson, Carol A.; Grodzinski, Bernard

    1992-01-01

    Sink to source transition parallels loss of thigmotropic capacity in tendrils of a semileafless mutant, Pisum sativum cv Curly. Macroscopic tendril development is subdivided based on thigmotropic capacity. Stage I is the elongation stage and, although the rate of photosynthesis is similar to that of stage II and III tendrils, dark respiration rates are higher in stage I. During stage II, tendrils are thigmotropic and act as a sink. Even though stage II tendrils have CO2 exchange characteristics similar to those of stage III tendrils, which are coiled, our fluorescein, 14C-partitioning, and 11C-translocation experiments suggest that stage I and II tendrils do not export carbon. Only stage III tendrils act as sources of newly fixed carbon. Export from them is blocked by cold, heat girdling of the petiole, or anoxia treatment of the tendrils. A late stage II tendril complex, in which coiling is occurring, may be exporting photoassimilates; however, this phenomenon can be attributed to the fact that the pea leaf is a compound structure and there may be one or more stage III tendrils, no longer thigmotropic, within the tendril complex. Photosynthetic maturity in pea tendrils occurs at stage III and is characterized by the ability of these tendrils to export photoassimilates. Images Figure 1 Figure 2 Figure 3 PMID:16653179

  12. Effects and action mechanisms of Korean pear (Pyrus pyrifolia cv. Shingo) on alcohol detoxification.

    PubMed

    Lee, Ho-Sun; Isse, Toyoshi; Kawamoto, Toshihiro; Woo, Hyun-Su; Kim, An Keun; Park, Jong Y; Yang, Mihi

    2012-11-01

    Korean pear (Pyrus pyrifolia cv. Shingo) has been used as a traditional medicine for alleviating alcohol hangover. However, scientific evidence for its effectiveness or mechanism is not clearly established. To investigate its mechanism of alcohol detoxification, both in vitro and in vivo studies were performed with an aldehyde dehydrogenase 2 (ALDH2) alternated animal model. The pear extract (10?mL/kg bw) was administered to Aldh2 normal (C57BL/6) and deficient (Aldh2 -/-) male mice. After 30?min, ethanol (1?g or 2?g/kg bw) was administered to the mice via gavage. Levels of alcohol and acetaldehyde in blood were quantified by GC/MS. First, it was observed that the pears stimulated both alcohol dehydrogenase (ADH) and ALDH activities by 2?3- ?and 1.3-fold in in vitro studies, respectively. Second, mouse PK data (AUC(?) and C(max) ) showed that the pear extract decreased the alcohol level in blood regardless of ALDH2 genotype. Third, the pear increased the acetaldehyde level in blood in Aldh2 deficient mice but not in Aldh2 normal mice. Therefore, the consistent in vitro and in vivo data suggest that Korean pears stimulate the two key alcohol-metabolizing enzymes. These stimulations could be the main mechanism of the Korean pear for alcohol detoxification. Finally, the results suggest that polymorphisms of human ALDH2 could bring out individual variations in the effects of Korean pear on alcohol detoxification. PMID:22451246

  13. Composition of Citrus sinensis (L.) Osbeck cv «Maltaise demi-sanguine» juice. A comparison between organic and conventional farming.

    PubMed

    Letaief, Hend; Zemni, Hassen; Mliki, Ahmed; Chebil, Samir

    2016-03-01

    Juices from conventionally and organically grown Citrus sinensis (L.) Osbeck cv. Maltaise demi-sanguine blood orange were investigated for quality parameters and antioxidant capacity. This blood orange variety is particularly rich in linoleic, linolenic acids, vitamin C and phenolic compounds. The quantitative determination of these compounds in cv. Maltaise demi-sanguine juice produced under conventional and organic agricultural practices revealed significant differences. The organically grown fruits contained more hesperidin and total fatty acids amounts as well as a higher sugar content and a lower acidity. Conventionally-grown fruit was found to have an increase in antioxidant capacity. In addition to having higher antioxidant activity conventionally-grown fruit had an observed increase in the concentration of phenolic acids and most flavonoids. The results of this study indicated that organically-grown Maltaise demi-sanguine juice contained an increased concentration of hesperidin which has been observed to possess biological activities associated with a healthy life. PMID:26471557

  14. CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Aqua-FM3_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  15. CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Aqua-FM4_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2005-03-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  16. CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Terra-FM2_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  17. CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Terra-FM1_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  18. Effects of Colonization of the Roots of Domestic Rice (Oryza sativa L. cv. Amaroo) by Burkholderia pseudomallei

    PubMed Central

    Constantinoiu, Constantin; Gardiner, Christopher; Warner, Jeffrey

    2015-01-01

    Burkholderia pseudomallei is a saprophytic bacterium that causes melioidosis and is often isolated from rice fields in Southeast Asia, where the infection incidence is high among rice field workers. The aim of this study was to investigate the relationship between this bacterium and rice through growth experiments where the effect of colonization of domestic rice (Oryza sativa L. cv Amaroo) roots by B. pseudomallei could be observed. When B. pseudomallei was exposed to surface-sterilized seeds, the growth of both the root and the aerosphere was retarded compared to that in controls. The organism was found to localize in the root hairs and endodermis of the plant. A biofilm formed around the root and root structures that were colonized. Growth experiments with a wild rice species (Oryza meridionalis) produced similar retardation of growth, while another domestic cultivar (O. sativa L. cv Koshihikari) did not show retarded growth. Here we report B. pseudomallei infection and inhibition of O. sativa L. cv Amaroo, which might provide insights into plant interactions with this important human pathogen. PMID:25911477

  19. Improved antioxidant and anti-inflammatory potential in mice consuming sour cherry juice (Prunus Cerasus cv. Maraska).

    PubMed

    Sari?, Ana; Sobocanec, Sandra; Balog, Tihomir; Kusi?, Borka; Sverko, Visnja; Dragovi?-Uzelac, Verica; Levaj, Branka; Cosi?, Zrinka; Macak Safranko, Zeljka; Marotti, Tatjana

    2009-12-01

    The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties. PMID:19763832

  20. Psychometric Properties of the Obsessive-Compulsive Inventory-Child Version (OCI-CV) in Chilean Children and Adolescents

    PubMed Central

    Martínez-González, Agustín E.; Rodríguez-Jiménez, Tíscar; Piqueras, José A.; Vera-Villarroel, Pablo; Godoy, Antonio

    2015-01-01

    In recent years, there has been a considerable increase in the development of assessment tools for obsessive-compulsive symptomatology in children and adolescents. The Obsessive Compulsive Inventory-Child Version (OCI-CV) is a well-established assessment self-report, with special interest for the assessment of dimensions of Obsessive Compulsive Disorder (OCD). This instrument has shown to be useful for clinical and non-clinical populations in two languages (English and European Spanish). Thus, the aim of this study was to analyze the psychometric properties of the OCI-CV in a Chilean community sample. The sample consisted of 816 children and adolescents with a mean age of 14.54 years (SD = 2.21; range = 10–18 years). Factor structure, internal consistency, test-retest reliability, convergent/divergent validity, and gender/age differences were examined. Confirmatory factor analysis showed a 6-factor structure (Doubting/Checking, Obsessing, Hoarding, Washing, Ordering, and Neutralizing) with one second-order factor. Good estimates of reliability (including internal consistency and test-retest), evidence supporting the validity, and small age and gender differences (higher levels of OCD symptomatology among older participants and women, respectively) are found. The OCI-CV is also an adequate scale for the assessment of obsessions and compulsions in a general population of Chilean children and adolescents. PMID:26317404

  1. Psychometric Properties of the Obsessive-Compulsive Inventory-Child Version (OCI-CV) in Chilean Children and Adolescents.

    PubMed

    Martínez-González, Agustín E; Rodríguez-Jiménez, Tíscar; Piqueras, José A; Vera-Villarroel, Pablo; Godoy, Antonio

    2015-01-01

    In recent years, there has been a considerable increase in the development of assessment tools for obsessive-compulsive symptomatology in children and adolescents. The Obsessive Compulsive Inventory-Child Version (OCI-CV) is a well-established assessment self-report, with special interest for the assessment of dimensions of Obsessive Compulsive Disorder (OCD). This instrument has shown to be useful for clinical and non-clinical populations in two languages (English and European Spanish). Thus, the aim of this study was to analyze the psychometric properties of the OCI-CV in a Chilean community sample. The sample consisted of 816 children and adolescents with a mean age of 14.54 years (SD = 2.21; range = 10-18 years). Factor structure, internal consistency, test-retest reliability, convergent/divergent validity, and gender/age differences were examined. Confirmatory factor analysis showed a 6-factor structure (Doubting/Checking, Obsessing, Hoarding, Washing, Ordering, and Neutralizing) with one second-order factor. Good estimates of reliability (including internal consistency and test-retest), evidence supporting the validity, and small age and gender differences (higher levels of OCD symptomatology among older participants and women, respectively) are found. The OCI-CV is also an adequate scale for the assessment of obsessions and compulsions in a general population of Chilean children and adolescents. PMID:26317404

  2. Quantifying the Deformation of Leoville Chondrules in 3D: Implications for the Post-Accretional History of the CV3 Parent Body

    NASA Astrophysics Data System (ADS)

    Almeida, N. V.; Smith, C. L.; Sykes, D.; Downes, H.; Ahmed, F.; Russell, S. S.

    2015-07-01

    Micro-CT scanning allows for the three-dimensional analysis of both degree of deformation and direction of preferred orientation of chondrules in the Leoville CV3 meteorite, indicating post-accretional impact as the cause for the foliation.

  3. Asteroid Lightcurve Analysis at Riverland Dingo Observatory (RDO): 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV 26.

    NASA Astrophysics Data System (ADS)

    Hills, Kevin

    2013-01-01

    Lightcurves for five asteroids selected from the Collaborative Asteroid Lightcurve Link (CALL) were obtained at Riverland Dingo Observatory (RDO) from 2012 July-September: 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV26.

  4. Establishment of regeneration and transformation system in Egyptian sesame (Sesamum indicum L.) cv Sohag 1.

    PubMed

    Al-Shafeay, Amal F; Ibrahim, Ahmed S; Nesiem, Mohamed R; Tawfik, Mohamed S

    2011-01-01

    Sesame (Sesamum indicum L.) is an important oil crop in many tropical and sub-tropical regions of the world, yet has received little attention in applying modern biotechnology in its improvement due to regeneration and transformation difficulties. Here within, we report the successful production of transgenic fertile plants of sesame (cv Sohag 1), after screening several cultivars. Agrobacterium tumefaciens- carrying the pBI121 plasmid {neomycin phosphotransferase gene (NPTII) and a ?-glucuronidase gene (GUS)} was used in all experiments. Recovery of transgenic sesame shoots was achieved using shoot induction medium (Murashige and Skoog MS basal salt mixture + Gamborg's B5 vitamins + 2.0 mg/l BA + 1.0 mg/l IAA + 5.0 mg/l AgNO3 + 30.0 g/l sucrose + 7.0 g/l agar + 200 mg/l cefotaxime and 25 mg/l kanamycin) and shoots were rooted on MS medium + B5 vitamins + 1.0 mg/l IAA + 10.0 g/l sucrose and 7.0 g/l agar. Rooted shoots were transplanted into soil and grown to maturity in greenhouse. Incorporation and expression of the GUS gene into T0 sesame plants was confirmed using polymerase chain reaction (PCR), reverse transcriptase-PCR (RT-PCR) and GUS histochemical assay. Several factors were found to be important for regeneration and transformation in sesame. The most effective were plant genotype and the addition of AgNO3 for successful recovery of sesame shoots. Co-cultivation time and optical density of the Agrobacterium were also critical for sesame transformation. This work is an attempt to open the door for further genetic improvement of sesame using important agronomic traits. PMID:22179191

  5. Scale-Dependent Measurements of Meteorite Strength and Fragmentation: Tamdakht (H5) and Allende (CV3)

    NASA Astrophysics Data System (ADS)

    Cotto-Figueroa, Desireé; Asphaug, Erik; Garvie, Laurence; Morris, Melissa; Rai, Ashwin; Chattopadhyay, Aditi; Chawla, Nikhilesh

    2015-11-01

    Meteorites are pieces of natural space debris, which have survived ejection from their parent bodies and passage through the Earth’s atmosphere. As such, they provide a unique opportunity to study the fundamental physical and mechanical properties of early Solar System materials. But to date, few direct studies of physical properties have been conducted on meteoritic materials, in contrast to extensive chemical and isotopic analyses. It is important to determine these properties as they are related to disruption and fragmentation of bolides and asteroids, and activities related to sample return and hazardous asteroid mitigation. Here we present results from an ongoing suite of scale-dependent studies of meteorite strength and fragmentation. The meteorites studied are Tamdakht (H5), an ordinary chondrite that exhibits a heterogeneous structure criss-crossed with shock veins and centimeter-sized regions of white and light grey, and the carbonaceous chondrite Allende (CV3), which suitable pieces are light grey with abundant chondrules and CAIs. Uniaxial compression tests are performed on meteorite cubes ranging from 0.5 to 4 centimeters using an Instron 5985 frame with a 250 kN load cell and compression fixtures with 145mm diameter radial platens. All tests are conducted at room temperature and in displacement control with a displacement rate of 0.25 mm per minute to ensure quasi-static conditions. A three-dimensional digital image correlation (DIC) system that enables noncontact measurement of displacement and strain fields is also used. Analysis of the strength and failure process of the two meteorite types is conducted and compared to terrestrial materials.

  6. Effect of quarantine treatments on the carbohydrate and organic acid content of mangoes (cv. Tommy Atkins)

    NASA Astrophysics Data System (ADS)

    Cruz, J. N.; Soares, C. A.; Fabbri, A. D. T.; Cordenunsi, B. R.; Sabato, S. F.

    2012-08-01

    Brazil is one of the largest mango producers and the third largest mango exporter worldwide. Irradiation treatment and its commercial feasibility have been studied in our country to make it possible to develop new markets and, consequently, to compete with the major exporters of mangoes, Mexico and India. This work was designed to compare irradiation treatment with the hot water dip treatment in mangoes cv. Tommy Atkins for export and to verify that the main attributes for acceptance, color and texture, as well as carbohydrate and organic acid contents, were maintained. In this study, the fruit was divided into groups: control, hot water dip-treated (46 °C for 90 min), and irradiation-treated at doses of 0.4 kGy and 1.0 kGy. The fruit was stored at low temperature (11 °C±2) for 14 days and then at room temperature (23 °C±2) until the end of the study. The results indicated that the fruit given a dose of 1.0 kGy remained in a less advanced stage of ripening (stage 3) throughout the storage period, but experienced a greater loss of texture in the beginning of the experiment. It was noted that only the control group had higher levels of citric acid and succinic acid on the last day of the experiment. There were no significant differences in the total sugar content between any treatment groups. Gamma radiation can be used as a quarantine treatment and does not interfere negatively with the quality attributes of mangoes.

  7. Nature and origin of interstellar diamond from the Allende CV3 meteorite

    NASA Technical Reports Server (NTRS)

    Blake, David; Freund, Friedemann; Bunch, Ted; Krishnan, Kannan; Stampfer, Mitch; Chang, Sherwood; Tielens, Alexander G. G. M.

    1990-01-01

    Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende CV3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on 3 mm EM grids. The AEM was performed on transmission-thin fragments of the material which overlay holes in the film, to eliminate interference from the substrate. Electron Spectroscopy for Chemical Analysis (ESCA) was performed on a large aliquot of C. Diamond was identified by selected area electron diffraction. Scanning Transmission Electron Microscope / Energy Dispersive X-ray (STEM-EDS) microanalyses of the C delta diamond, using a light-element detector, show that oxygen and possibly nitrogen are the only impurities consistently present. ESCA spectra from bulk C delta material confirm the presence of N at a level of 0.35 percent or less. Under UV irradiation a yellow-red fluorescence is observed, consistent with that of natural diamonds containing substitutional N. Electron Energy Loss Spectra (EELS) were recorded at 2 eV resolution from the C delta diamond, high pressure synthetic diamond, a diamond film produced in a low pressure plasma by chemical vapor deposition (CVD) on a heated silicon substrate (Roy, 1987), graphite, and amorphous arc sputtered carbon. Comparison of the carbon K edge shape and fine structure shows the Allende C delta phase to be largely diamond, but with a significant pre-edge absorption feature indicative of transitions of C 1s electrons into pi asterisk orbitals which are absent in the purely sp(3)-bonded diamond but present in graphite and amorphous carbon.

  8. Diurnal cycles of embolism formation and repair in petioles of grapevine (Vitis vinifera cv. Chasselas).

    PubMed

    Zufferey, V; Cochard, H; Ameglio, T; Spring, J-L; Viret, O

    2011-07-01

    The impact of water deficit on stomatal conductance (g(s)), petiole hydraulic conductance (K(petiole)), and vulnerability to cavitation (PLC, percentage loss of hydraulic conductivity) in leaf petioles has been observed on field-grown vines (Vitis vinifera L. cv. Chasselas). Petioles were highly vulnerable to cavitation, with a 50% loss of hydraulic conductivity at a stem xylem water potential (?(x)) of -0.95?MPa, and up to 90% loss of conductivity at a ?(x) of -1.5?MPa. K(petiole) described a daily cycle, decreasing during the day as water stress and evapotranspiration increased, then rising again in the early evening up to the previous morning's K(petiole) levels. In water-stressed vines, PLC increased sharply during the daytime and reached maximum values (70-90%) in the middle of the afternoon. Embolism repair occurred in petioles from the end of the day through the night. Indeed, PLC decreased in darkness in water-stressed vines. PLC variation in irrigated plants showed the same tendency, but with a smaller amplitude. The Chasselas cultivar appears to develop hydraulic segmentation, in which petiole cavitation plays an important role as a 'hydraulic fuse', thereby limiting leaf transpiration and the propagation of embolism and preserving the integrity of other organs (shoots and roots) during water stress. In the present study, progressive stomatal closure responded to a decrease in K(petiole) and an increase in cavitation events. Almost total closure of stomata (90%) was measured when PLC in petioles reached >90%. PMID:21447755

  9. Tradescantia pallida cv. purpurea boom in the characterization of air pollution by accumulation of trace elements.

    PubMed

    Sumita, Nairo Massakazu; Mendes, Maria Elizabete; Macchione, Mariangela; Guimarães, Eliane Tigre; de Lichtenfels, Ana Julia Faria Coimbra; de Lobo, Débora-Jã Araujo; Saldiva, Paulo Hilárlo Nascimento; Saiki, Mitiko

    2003-05-01

    Tradescantia pallida cv. purpurea, a plant species widely employed for ornamentation in Brazil, has been successfully used for monitoring the genotoxicity of various agents by the micronucleus assay. To amplify knowledge about its suitability as a bioindicator species, its capacity for accumulating trace elements from urban air pollution was evaluated. T. pallida was rooted using standardized soil, and the vases were distributed in two highly polluted sites of the urban area of São Paulo, Brazil (Cerqueira Cesar and Congonhas districts), and in one unpolluted control site situated approximately 50 km from downtown São Paulo (in Caucaia do Alto). Approximately six months after exposure to pollutants, adult leaves of this plant were collected monthly for 12 months. The leaves were washed with deionized water, dried, and ground for analyses. Characterization of element levels was carried out by neutron activation analysis. Powdered samples and standards were irradiated at the IEA-R1 nuclear reactor for short and long periods, and concentrations of As, Ba, Br, Ca, Ce, Cl, Cr, Co, Fe, K, La, Mn, Na, Rb, Sb, Sc, Sr, Th, and Zn were determined. Analysis of variance applied to the results indicated that samples from polluted sites present the highest concentrations of Ba, Ce, Cr, Co, Fe, La, Sb, and Sc (p < 0.05). Discriminant analysis revealed that it was possible to distinguish the two polluted areas with a precision of 97.5%, based on the amount of pollutant elements measured in the plants at each site. The results indicated the potential use of T. pallida as an accumulator plant for air pollution biomonitoring. PMID:12774990

  10. A secondary origin of chondrule magnetization in the Allende CV carbonaceous chondrite

    NASA Astrophysics Data System (ADS)

    Carporzen, L.; Fu, R.; Andrade Lima, E.; Weiss, B. P.

    2011-12-01

    Magnetic fields in the solar nebula may have played a key role in the radial transport of angular momentum and mass during the early accretional phase of the solar system. Chondrules and many calcium aluminum inclusions (CAIs), millimeter sized silicate objects found in most chondritic meteorites, were heated to high temperatures and cooled in the nebula and therefore may have recorded a thermoremanent magnetization (TRM) from the nebula field. Additionally, primitive magnetization in chondrules and CAIs may yield constraints about their mode of formation. However, any such primary magnetization may have been significantly altered during subsequent metamorphism and aqueous alteration on the parent asteroid. We performed two tests to determine the nebular origins of remanent magnetization in chondrules and refractory inclusions in the Allende CV3 carbonaceous chondrite: 1) a classic paleomagnetic conglomerate test to identify post-accretional remagnetization events and 2) a unidirectionality test of subsamples taken from individual chondrules and CAIs. We conducted individual measurements of mutually oriented chondrules, CAIs, and matrix as well as SQUID microscope maps of the magnetic fields of 30 ?m thin sections. All samples and thin sections were mutually oriented to within 5°. Our results confirm previous findings that all subsamples of the meteorite carry a unidirectional overprint blocked up to 260°-290°C (MT component). Chondrules and CAIs also carry a higher temperature (HT) remanence oriented in scattered directions unrelated to the direction of the MT overprint. We have confirmed that this HT magnetization is not an artifact of the demagnetization procedure but is a preterrestrial component. Measurements of subsamples of single chondrules and CAIs show that the HT magnetization is not unidirectional within each inclusion. Petrographic data suggests that most magnetic minerals in Allende were the product of parent body alteration. These facts suggest that the magnetization in Allende chondrules and CAIs is not a preaccretional TRM and is unlikely to record the nebular field. We suggest that preaccretional remanence in Allende material was destroyed by aqueous alteration and metasomatism, which was followed or coincident with metamorphism that introduced the MT overprint. The HT component we detect may represent a chemical remanent magnetization (CRM) acquired during aqueous alteration.

  11. Growth promotion and colonization of switchgrass (Panicum virgatum) cv. Alamo by bacterial endophyte Burkholderia phytofirmans strain PsJN

    PubMed Central

    2012-01-01

    Background Switchgrass is one of the most promising bioenergy crop candidates for the US. It gives relatively high biomass yield and can grow on marginal lands. However, its yields vary from year to year and from location to location. Thus it is imperative to develop a low input and sustainable switchgrass feedstock production system. One of the most feasible ways to increase biomass yields is to harness benefits of microbial endophytes. Results We demonstrate that one of the most studied plant growth promoting bacterial endophytes, Burkholderia phytofirmans strain PsJN, is able to colonize and significantly promote growth of switchgrass cv. Alamo under in vitro, growth chamber, and greenhouse conditions. In several in vitro experiments, the average fresh weight of PsJN-inoculated plants was approximately 50% higher than non-inoculated plants. When one-month-old seedlings were grown in a growth chamber for 30 days, the PsJN-inoculated Alamo plants had significantly higher shoot and root biomass compared to controls. Biomass yield (dry weight) averaged from five experiments was 54.1% higher in the inoculated treatment compared to non-inoculated control. Similar results were obtained in greenhouse experiments with transplants grown in 4-gallon pots for two months. The inoculated plants exhibited more early tillers and persistent growth vigor with 48.6% higher biomass than controls. We also found that PsJN could significantly promote growth of switchgrass cv. Alamo under sub-optimal conditions. However, PsJN-mediated growth promotion in switchgrass is genotype specific. Conclusions Our results show B. phytofirmans strain PsJN significantly promotes growth of switchgrass cv. Alamo under different conditions, especially in the early growth stages leading to enhanced production of tillers. This phenomenon may benefit switchgrass establishment in the first year. Moreover, PsJN significantly stimulated growth of switchgrass cv. Alamo under sub-optimal conditions, indicating that the use of the beneficial bacterial endophytes may boost switchgrass growth on marginal lands and significantly contribute to the development of a low input and sustainable feedstock production system. PMID:22647367

  12. Opaque Mineral Assemblages at Chondrule Boundaries in the Vigarano CV Chondrite: Evidence for Gas-Solid Reactions Following Chondrule Formation

    NASA Technical Reports Server (NTRS)

    Lauretta, Dante S.

    2004-01-01

    Recent studies of opaque minerals in primitive ordinary chondrites suggest that metal grains exposed at chondrule boundaries were corroded when volatile elements recondensed after the transient heating event responsible for chondrule formation. Metal grains at chondrule boundaries in the Bishunpur (LL3.1) chondrite are rimmed by troilite and fayalite. If these layers formed by gas solid reaction, then the composition of the corrosion products can provide information on the chondrule formation environment. Given the broad similarities among chondrules from different chondrite groups, similar scale layers should occur on chondrules in other primitive meteorite groups. Here I report on metal grains at chondrule boundaries in Vigarano (CV3).

  13. Summer pruning: an ecological alternative to postharvest calcium treatment to improve storability of high quality apple cv. 'Reinette du Canada'.

    PubMed

    Guerra, M; Casquero, P A

    2010-08-01

    Two strategies, summer pruning and postharvest Ca treatment, were studied in apple (Malus domestica Borkh) cv. 'Reinette du Canada' in order to analyze its effect on the fruit quality during storage. Summer pruning and Ca treatment reduced external and internal bitter-pits; so after 180 days of storage, both treatments decreased external bitter-pit by 10.0% and 16.7%, respectively. Summer pruning influenced color, firmness, total soluble solids and titratable acidity (TA) of fruit during storage, whereas Ca treatment only affected firmness and TA. Fruit from pruned trees had significant lower K and Mg than those from unpruned trees and Ca treatment increased Ca content. Orchard management, by means of summer pruning, combined with Ca postharvest application would be useful to prevent losses due to bitter-pit during storage in commercial orchards. However, in organic orchards, summer pruning would be the ecological alternative to decrease bitter-pit incidence during storage in high quality apple cv. 'Reinette du Canada'. K/Ca ratio, on the peel at harvest, turned out to be the best parameter to correlate with external and internal bitter-pits during storage; so this ratio would be useful to predict bitter-pit on long-term storage. PMID:21339152

  14. Studies on quality and vase life of cut Gerbera jamesonii cv. 'Balance' flowers by silver nanoparticles and chlorophenol.

    PubMed

    Safa, Zakieh; Hashemabadi, Davood; Kaviani, Behzad; Nikchi, Narges; Zarchini, Mohammad

    2015-03-01

    Cut gerbera flowers are sensitive to microbial contamination and have a short vase life. Silver nanoparticles are used in various applications as an antimicrobial agent. An experiment was conducted to determine the effect of different concentrations of SNP and chlorophenol to extend the vase life and postharvest quality of gerbera (Gerberajamesonii cv. 'Balance') cut flowers. Cut gerbera flowers were kept in solutions containing 0, 5, 10 and 20 mg l(-1) SNP and/or 0, 5 and 10 mM chlorophenol for 24 hr; then held in vase solution containing 250 mg l(-1) 8-hydroxyquinoline sulphate and 3% sucrose. The maximum vase life (16.33 days) was observed in flowers held in solution containing 10 mg l(-1) SNP. The 5 mg l(-1) SNP plus 10 mM chlorophenol and 10 mg l(-1) SNP plus 5 mM chlorophenol inhibited bacterial growth in the vase solution. The minimum fresh weight loss (6.48 gr) during the vase period was observed for flowers kept in solution containing 20 mg l(-1)1 SNP. The results revealed that SNP and chlorophenol have the potential to extend vase life and enhanc the postharvest quality of cut gerbera cv. 'Balance' flowers. PMID:25895266

  15. Processor core for real time background identification of HD video based on OpenCV Gaussian mixture model algorithm

    NASA Astrophysics Data System (ADS)

    Genovese, Mariangela; Napoli, Ettore

    2013-05-01

    The identification of moving objects is a fundamental step in computer vision processing chains. The development of low cost and lightweight smart cameras steadily increases the request of efficient and high performance circuits able to process high definition video in real time. The paper proposes two processor cores aimed to perform the real time background identification on High Definition (HD, 1920 1080 pixel) video streams. The implemented algorithm is the OpenCV version of the Gaussian Mixture Model (GMM), an high performance probabilistic algorithm for the segmentation of the background that is however computationally intensive and impossible to implement on general purpose CPU with the constraint of real time processing. In the proposed paper, the equations of the OpenCV GMM algorithm are optimized in such a way that a lightweight and low power implementation of the algorithm is obtained. The reported performances are also the result of the use of state of the art truncated binary multipliers and ROM compression techniques for the implementation of the non-linear functions. The first circuit has commercial FPGA devices as a target and provides speed and logic resource occupation that overcome previously proposed implementations. The second circuit is oriented to an ASIC (UMC-90nm) standard cell implementation. Both implementations are able to process more than 60 frames per second in 1080p format, a frame rate compatible with HD television.

  16. Petrology, mineralogy, and oxygen isotope compositions of aluminum-rich chondrules from CV3 chondrites

    NASA Astrophysics Data System (ADS)

    Wang, Ying; Hsu, Weibiao; Li, Xianhua; Li, Qiuli; Liu, Yu; Tang, Guoqiang

    2015-12-01

    Bulk major element composition, petrography, mineralogy, and oxygen isotope compositions of twenty Al-rich chondrules (ARCs) from five CV3 chondrites (Northwest Africa [NWA] 989, NWA 2086, NWA 2140, NWA 2697, NWA 3118) and the Ningqiang carbonaceous chondrite were studied and compared with those of ferromagnesian chondrules and refractory inclusions. Most ARCs are marginally Al-richer than ferromagnesian chondrules with bulk Al2O3 of 10-15 wt%. ARCs are texturally similar to ferromagnesian chondrules, composed primarily of olivine, pyroxene, plagioclase, spinel, Al-rich glass, and metallic phases. Minerals in ARCs have intermediate compositions. Low-Ca pyroxene (Fs0.6-8.8Wo0.7-9.3) has much higher Al2O3 and TiO2 contents (up to 12.5 and 2.3 wt%, respectively) than that in ferromagnesian chondrules. High-Ca pyroxene (Fs0.3-2.0Wo33-54) contains less Al2O3 and TiO2 than that in Ca,Al-rich inclusions (CAIs). Plagioclase (An77-99Ab1-23) is much more sodic than that in CAIs. Spinel is enriched in moderately volatile element Cr (up to 6.7 wt%) compared to that in CAIs. Al-rich enstatite coexists with anorthite and spinel in a glass-free chondrule, implying that the formation of Al-enstatite was not due to kinetic reasons but is likely due to the high Al2O3/CaO ratio (7.4) of the bulk chondrule. Three ARCs contain relict CAIs. Oxygen isotope compositions of ARCs are also intermediate between those of ferromagnesian chondrules and CAIs. They vary from -39.4‰ to 13.9‰ in ?18O and yield a best fit line (slope = 0.88) close to the carbonaceous chondrite anhydrous mineral (CCAM) line. Chondrules with 5-10 wt% bulk Al2O3 have a slightly more narrow range in ?18O (-32.5 to 5.9‰) along the CCAM line. Except for the ARCs with relict phases, however, most ARCs have oxygen isotope compositions (>-20‰ in ?18O) similar to those of typical ferromagnesian chondrules. ARCs are genetically related to both ferromagnesian chondrules and CAIs, but the relationship between ARCs and ferromagnesian chondrules is closer. Most ARCs were formed during flash heating and rapid cooling processes like normal chondrules, only from chemically evolved precursors. ARCs extremely enriched in Al and those with relict phases could have had a hybrid origin (Krot et al. 2002) which incorporated refractory inclusions as part of the precursors in addition to ferromagnesian materials. The occurrence of melilite in ARCs indicates that melilite-rich CAIs might be present in the precursor materials of ARCs. The absence of melilite in most ARCs is possibly due to high-temperature interactions between a chondrule melt and the solar nebula.

  17. Identification and Expression of Two Novel Cytochrome P450 Genes, CYP6CV1 and CYP9A38, in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

    PubMed Central

    Chen, Jun; Li, Chuan; Yang, Zhifan

    2015-01-01

    Cnaphalocrocis medinalis Güenée can cause severe losses in rice. Cytochrome P450s play crucial roles in the metabolism of allelochemicals in herbivorous insects. Two novel P450 cDNAs, CYP6CV1 and CYP9A38, were cloned from the midgut of C. medinalis. CYP6CV1 encodes a protein of 500 amino acid residues, while CYP9A38-predicted protein has 531 amino acid residues. Both cDNA-predicted proteins contain the conserved functional domains for all P450s. Phylogenetic analyses showed that CYP6CV1 is grouped in the cluster containing CYP6B members, while CYP9A38 is in the cluster including CYP9 members. However, both clusters are contained in the same higher lineage. Homologous analysis revealed that CYP6CV1 is most similar to CYP6B8, CYP6B7, CYP6B6, CYP6B2, and CYP6B4 with the highest amino acid identity of 41%. CYP9A38 is closest to CYP9A17, CYP9A21, CYP9A20, and CYP9A19 with the highest amino acid identity of 66%. Studies of temporal expression profiles revealed that CYP9A38 showed a steady increase in mRNA level during the five instar stages, but a low-expression level in pupae, and then presented at a high-expression level again in adults. Similar expression patterns were obtained with CYP6CV1. In the fifth instar larvae, CYP6CV1 was mainly expressed in midgut and fat bodies, whereas CYP9A38 was mainly expressed in midgut. Expression studies also revealed a 3.20-fold over-expression of CYP6CV1 and 3.54-fold over-expression of CYP9A38 after larval exposure to host rice resistance. Our results suggest that both CYP6CV1 and CYP9A38 may be involved in detoxification of rice phytochemicals. PMID:25896119

  18. Role of ethrel in causation of floral malformation in mango cv. Amrapali: A scanning electron microscopy study

    PubMed Central

    Singh, Archana; Ansari, Mohammad Wahid; Singh, C P; Shukla, Alok; Pant, Ramesh Chandra; Bains, Gurdeep

    2015-01-01

    Floral malformation is a main constraint to reduce fruit yield in mango plants. Recently, we report on the role of putrescine in normalizing the functional morphology of mango flower by reducing various adverse effects of ethylene. Here, ethrel, an ethylene releasing compound, was exogenously applied to mango plant cv Amrapali to evaluate the response of flower development under high level of ethylene. Scanning electron microscopy (SEM) study showed that ethrel treated flowers were observed to progressively be deformed and remain unbloom. The flower buds were not distinguishable and flower parts such as petals, sepals, anther and stigma were not properly developed. The stamen showed fused anther lobes and carpel depicted curved style with pointed stigma. The findings of present study suggest the involvement of ethylene to abort the functional morphology of flower and thereby development of malformation. PMID:25751309

  19. Anatalline and other methyl jasmonate-inducible nicotine alkaloids from Nicotiana tabacum cv. By-2 cell cultures.

    PubMed

    Häkkinen, Suvi T; Rischer, Heiko; Laakso, Into; Maaheimo, Hannu; Seppänen-Laakso, Tuulikki; Oksman-Caldentey, Kirsi-Marja

    2004-10-01

    Anatalline [2,4-di(3-pyridyl)piperidine] accumulation was shown to be induced by methyl jasmonate in Nicotiana tabacum cv. BY-2 cell cultures. Beside anatabine, anatalline represented the most abundant alkaloid, moreover, it was always present in two isomeric forms occurring always in similar concentrations. Both isomers could be completely separated by GC-MS. For structural analysis, the isolation of both isomers was performed using a semi-preparative HPLC system. The structures of anatalline [cis-2,4-di(3-pyridyl)piperidine] and its stereoisomer trans-2,4-di(3-pyridyl)piperidine were confirmed by MS and 1D and 2D NMR spectral data. The biosynthetic origin of anatalline was studied by feeding alkaloid precursors to BY-2 cell cultures. PMID:15490322

  20. Petrography and classification of Ca, Al-rich and olivine-rich inclusions in the Allende CV3 chondrite

    NASA Technical Reports Server (NTRS)

    Kormacki, A. S.; Wood, J. A.

    1984-01-01

    The results of a detailed, systematic petrographic survey of Ca, Al-rich and olivine-rich inclusions in the Allende CV3 chondrite are reported, and a new classification system based on clearly defined and readily applied petrographic criteria is presented. Most Allende inclusions are aggregates containing one or more of three distinct constituents: (1) rimmed concentric objects enriched in Al- and Ti-rich oxide minerals and various amounts of Ca-rich silicates; (2) porous, 'fine-grained' chaotic material enriched in Ca-rich silicates, especially clinopyroxenes and garnets; and (3) porous, 'fine-grained', mafic inclusion matrix, enriched in olivine, pyroxene, and feldspathoids. Two texturally distinct varieties of inclusions consist primarily of inclusion matrix: unrimmed olivine aggregates and rimmed olivine aggregates. Ca, Al-rich inclusions are classified on the basis of the size and abundance of their constituent concentric objects. Some fundamental relationships among Allende inclusions that previusly have not been emphasized are discussed.

  1. Complete genome sequence of a banana bract mosaic virus isolate infecting the French plantain cv. Nendran in India.

    PubMed

    Balasubramanian, V; Selvarajan, R

    2012-02-01

    The first complete genome sequence of an Indian isolate (TRY) of Banana bract mosaic virus (BBrMV) was determined following virus RNA extraction from the French plantain cv. Nendran (AAB). The complete genome was 9711 nucleotides excluding the poly(A) tail and had a genome organization similar to that of a Philippine (PHI) isolate characterized earlier. When compared to BBrMV-PHI, the complete genome sequence of BBrMV-TRY was 94% identical at the nucleotide level and its ten mature proteins had amino acid sequence identities ranging from 88 to 98%. Phylogenetic analysis suggests that the BBrMV-TRY isolate is closely related to the BBrMV-PHI isolate. PMID:22134527

  2. Physiological and biochemical responses resulting from nitrite accumulation in tomato (Lycopersicon esculentum Mill. cv. Ibiza F1).

    PubMed

    Ezzine, Monia; Ghorbel, Mohamed Habib

    2006-10-01

    The sensitivity of hydroponically cultivated tomato (Lycopersicon esculentum Mill. cv. Ibiza F1) submitted to nitrite treatments (0.25-10mM KNO(2)) for 7d was studied. Increasing nitrite levels in the culture medium led to several disruptions of tomato plants, reflected by reductions of both dry matter per plant, chlorophyll concentrations and the appearance of chlorosis symptoms at the leaf surface. This behaviour was accompanied by stimulation of nitrite, nitrate and ammonia ion accumulation, mainly in roots and old leaves. Higher proteolytic and gaiacol peroxidase (GPX, EC. 1.11.1.7) activities and malonyldialdehyde content were also noted. Protein content of the different plant organs was decreased by nitrite treatment. These physiological and biochemical parameters were chosen as they are stress indicators. Taken together, our data partly explain the harmful effects of nitrite ions, when excessive in the culture medium. PMID:16971215

  3. Development and validation of the Chinese Version of Indiana Job Satisfaction Scale (CV-IJSS) for people with mental illness.

    PubMed

    Tsang, Hector W H; Wong, Alvin

    2005-06-01

    Job satisfaction has been increasingly regarded as an important outcome of vocational rehabilitation programs among people with mental illness. Chinese measures of job satisfaction for individuals with mental illness are however extremely scarce. The aim of this study was to translate the 32-item Indiana Job Satisfaction Scale to Chinese. By means of the expert panel method, the culturally relevant 28-item Chinese Version of Indiana Job Satisfaction Scale (CV-IJSS) was then finalized. A validation study among a group of 125 individuals with mental illness showed that the scale had acceptable psychometric properties. Coefficient alpha of the total score was 0.81 with subscales ranging from 0.63 to 0.87. Test re-test reliability as measured by ICC was 0.77 for the total score and ranged from 0.54 to 0.72 for the subscales. Factorial analysis yielded a four factor solution (general satisfaction, job ambiguity and stress, advancement and security, and job recognition) accounting for 44% of the total variance. The factor solution had similarities as well as differences when compared with the Indiana Job Satisfaction Scale. The differences are discussed in the light of cultural differences. Relationship between scores of CV-IJSS and work performance, quality of life and self-esteem was positive in general which may act as evidence to its concurrent validity. The Chinese Version of Indiana Job Satisfaction Scale is ready for use by rehabilitation professionals to assess vocational rehabilitation outcome for individuals with mental illness in Hong Kong and other Chinese societies. Directions for further studies are suggested. PMID:16048246

  4. CvManGO, a method for leveraging computational predictions to improve literature-based Gene Ontology annotations.

    PubMed

    Park, Julie; Costanzo, Maria C; Balakrishnan, Rama; Cherry, J Michael; Hong, Eurie L

    2012-01-01

    The set of annotations at the Saccharomyces Genome Database (SGD) that classifies the cellular function of S. cerevisiae gene products using Gene Ontology (GO) terms has become an important resource for facilitating experimental analysis. In addition to capturing and summarizing experimental results, the structured nature of GO annotations allows for functional comparison across organisms as well as propagation of functional predictions between related gene products. Due to their relevance to many areas of research, ensuring the accuracy and quality of these annotations is a priority at SGD. GO annotations are assigned either manually, by biocurators extracting experimental evidence from the scientific literature, or through automated methods that leverage computational algorithms to predict functional information. Here, we discuss the relationship between literature-based and computationally predicted GO annotations in SGD and extend a strategy whereby comparison of these two types of annotation identifies genes whose annotations need review. Our method, CvManGO (Computational versus Manual GO annotations), pairs literature-based GO annotations with computational GO predictions and evaluates the relationship of the two terms within GO, looking for instances of discrepancy. We found that this method will identify genes that require annotation updates, taking an important step towards finding ways to prioritize literature review. Additionally, we explored factors that may influence the effectiveness of CvManGO in identifying relevant gene targets to find in particular those genes that are missing literature-supported annotations, but our survey found that there are no immediately identifiable criteria by which one could enrich for these under-annotated genes. Finally, we discuss possible ways to improve this strategy, and the applicability of this method to other projects that use the GO for curation. DATABASE URL: http://www.yeastgenome.org. PMID:22434836

  5. Physical Analysis of the Complex Rye (Secale cereale L.) Alt4 Aluminium (Aluminum) Tolerance Locus Using a Whole-Genome BAC Library of Rye cv. Blanco

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rye is a diploid crop species with many outstanding qualities, and is also important as a source of new traits for wheat and triticale improvement. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies. The library provides a 6 × genome ...

  6. Survey of 42,000 Gossypium hirsutum cv. Maxxa BAC-End Sequences and Frequency, Type, and Annotation of BAC-derived SSRs.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The quest for more molecular markers is a major initiative in cotton, which lags behind crops such as soybean, maize, and rice in this type of research. In an effort to increase the number of microsatellite markers in Gossypium, BAC-end sequences from a publicly available Gossypium hirsutum cv. Maxx...

  7. The Hewlett-Packard HP-41CV Hand-Held Computer as a Medium for Teaching Mathematics to Fire Control Systems Repairers. Research Report 1408.

    ERIC Educational Resources Information Center

    Boldovici, John A.; Scott, Thomas D.

    A study compared the benefits of using the Hewlett-Packard HP-41CV hand-held computer, as opposed to conventional training without computers, in teaching mathematics to fire control systems repairers. Thirty soldiers in a course to train fire control systems repairers received training in technical mathematics using the hand-held computer, whereas…

  8. Case 6:08-cv-00088-LED Document 409 Filed 10/01/10 Page 1 of 2 PageID #: 37793 IN THE UNITED STATES DISTRICT COURT

    E-print Network

    Shamos, Michael I.

    § § § § § § § § § Plaintiff vs. CASE NO. 6:08 CV 88 APPLE, INC. Defendant VERDICT FORM In answering these questions, you PATENTS 1A. Did Mirror Worlds prove by a preponderance ofthe evidence that Apple infringed any ofthe and convincing evidence that Apple's infringement, ifany, was willful? Answer "Yes" or "No" as to each patent

  9. Omega Gliadin Genes Expressed in Triticum Aestivum cv Butte 86: Effects of Post-anthesis Fertilizer on Transcript Accumulation During Grain Development

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The partial coding sequences of omega gliadin genes expressed in developing wheat kernels Triticum aestivum cv Butte 86 were identified in EST databases. Three gene assemblies encode proteins with PQQPFP as the predominant repetitive motif. Of these, two encode proteins with at least one cysteine an...

  10. Differences in Stylet Penetration Behaviors of Glassy-winged Sharpshooters on Xylella-Resistant Vitis candicans vs. Susceptible Vitis vinifera cv. ‘Chardonnay’

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Electrical penetration graph (EPG) monitoring was used to compare stylet penetration behaviors of glassy-winged sharpshooter (GWSS), a vector of Xylella fastidiosa (Xf), on Xf-resistant Vitis candicans grape vs. susceptible V. vinifera cv. ‘Chardonnay.’ Frequency of occurrence of X waves (represent...

  11. Improving the french fry quality of russeted potatoes through transformation with the anti-sweetening gene (UgpA) from the Chipping cv. Snowden

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Microtubers of two dual-purpose russeted potatoes were transformed with the anti-sweetening gene (UgpA) from the cv. Snowden using well know Agrobacterium tumifaciens mediated transformation system. Seventy-two and twenty-four distinct transformants of AOND95292-3Russ and ND7882b-7Russ, respectivel...

  12. Effects of Vaccination with 10-Valent Pneumococcal Non-Typeable Haemophilus influenza Protein D Conjugate Vaccine (PHiD-CV) on the Nasopharyngeal Microbiome of Kenyan Toddlers

    PubMed Central

    Feazel, Leah M.; Santorico, Stephanie A.; Robertson, Charles E.; Bashraheil, Mahfudh; Scott, J. Anthony G.

    2015-01-01

    Objective Pneumococcal conjugate vaccines reduce the prevalence of vaccine serotypes carried in the nasopharynx. Because this could alter carriage of other potential pathogens, we assessed the nasopharyngeal microbiome of children who had been vaccinated with 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). Methods Profiles of the nasopharyngeal microbiota of 60 children aged 12-59 months, who had been randomized to receive 2 doses of PHiD-CV (n=30) or Hepatitis A vaccine (n=30) 60 days apart, were constructed by 16S rRNA gene pyrosequencing of swab specimens collected before vaccination and 180 days after dose 1. Results Prior to vaccination, Moraxella catarrhalis (median of 12.3% of sequences/subject), Streptococcus pneumoniae (4.4%) and Corynebacterium spp. (5.6%) were the most abundant nasopharyngeal bacterial species. Vaccination with PHiD-CV did not significantly alter the species composition, abundance, or prevalence of known pathogens. Distinct microbiomes were identified based on the abundances of Streptococcus, Moraxella, and Haemophilus species. These microbiomes shifted in composition over the study period and were independent of age, sex, school attendance, antibiotic exposure, and vaccination. Conclusions Vaccination of children with two doses of PHiD-CV did not significantly alter the nasopharyngeal microbiome. This suggests limited replacement carriage with pathogens other than non-vaccine strains of S. pneumoniae. Trial Registration clinicaltrials.gov NCT01028326 PMID:26083474

  13. Effects of pH, sample size, and solvent partitioning on recovery of soluble phenolic acids and isoflavonoids in red clover (Trifolium pratense cv. Kenland)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Several extraction parameters were tested to determine optimal conditions for extracting phenolics from red clover (Trifolium pratense L. cv. Kenland). HPLC-UV profiles were compared before and after partitioning a methanolic extract of soluble phenolics with ethyl acetate-ether (1:1, v/v). The e...

  14. USE OF REPEATED BRONCHOALVEOLAR LAVAGE IN RABBITS TO ASSESS POLLUTANT-INDUCED LUNG CHANGES IN AN ANIMAL MODEL OF CARDIOVASCULAR (CV) DISEASE.

    EPA Science Inventory

    Animal models of coronary heart disease (e.g., hyperlipidemic rabbits) are being used to investigate epidemiologic associations between higher levels of air pollution and adverse CV consequences. Mechanisms by which pollutant-induced lung or systemic inflammation leads to acute C...

  15. A study of the effects of ALD growth temperature on Al2O3 gate oxide by C-V and G-V measurements

    E-print Network

    California at Los Angles, University of

    A study of the effects of ALD growth temperature on Al2O3 gate oxide by C-V and G-V measurements) Atomic layer deposition (ALD) was used to grow aluminum oxide (Al2O3) on n-type Si (100) substrates time, progress in low temperature ALD meth- ods has aroused interest in the relationships between de

  16. Draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi).

    PubMed

    Kolton, Max; Green, Stefan J; Harel, Yael Meller; Sela, Noa; Elad, Yigal; Cytryn, Eddie

    2012-10-01

    Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

  17. Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability

    PubMed Central

    2013-01-01

    Background Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation. Reviewer This article was reviewed by Sándor Pongor, L. Aravind and Anthony M. Poole. PMID:23324115

  18. CV -Teodoro Stadler, 2013 Teodoro Stadler -CV

    E-print Network

    Maxwell, Bruce D.

    of a total project budget of US$ 8,213,170.- Design and conduct research on different aspects of boll weevil): Austrian and Argentinean EDUCATION: Ph.D., Biological Sciences. 1988. Department of Biology. Faculty, pesticide resistance, development and testing of biorational pesticides based on natural products (inorganic

  19. The chemical composition of suberin in apoplastic barriers affects radial hydraulic conductivity differently in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix).

    PubMed

    Schreiber, Lukas; Franke, Rochus; Hartmann, Klaus-Dieter; Ranathunge, Kosala; Steudle, Ernst

    2005-05-01

    Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degradation (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were analysed quantitatively by gas chromatography and mass spectrometry. In corn, suberin was determined for isolated endodermal (ECW) and rhizo-hypodermal (RHCW) cell walls. In rice, the strong lignification of the central cylinder (CC), did not allow the isolation of endodermal cell walls. Similarly, exodermal walls could not be separated from the rhizodermal and sclerenchyma cell layers. Suberin analyses of ECW and RHCW of rice, thus, refer to either the entire CC or to the entire outer part of the root (OPR), the latter lacking the inner cortical cell layer. In both species, aromatic suberin was mainly composed of coumaric and ferulic acids. Aliphatic suberin monomers released from rice and corn belonged to five substance classes: primary fatty acids, primary alcohols, diacids, omega-hydroxy fatty acids, and 2-hydroxy fatty acids, with omega-hydroxy fatty acids being the most prominent substance class. Qualitative composition of aliphatic suberin of rice was different from that of corn; (i) it was much less diverse, and (ii) besides monomers with chain lengths of C(16), a second maximum of C(28) was evident. In corn, C(24) monomers represented the most prominent class of chain lengths. When suberin quantities were related to surface areas of the respective tissues of interest (hypodermis and/or exodermis and endodermis), exodermal cell walls of rice contained, on average, six-times more aliphatic suberin than those of corn. In endodermal cell walls, amounts were 34 times greater in rice than in corn. Significantly higher amounts of suberin detected in the apoplastic barriers of rice corresponded with a substantially lower root hydraulic conductivity (Lp(r)) compared with corn, when water flow was driven by hydrostatic pressure gradients across the apoplast. As the OPR of rice is highly porous and permeable to water, it is argued that this holds true only for the endodermis. The results imply that some caution is required when discussing the role of suberin in terms of an efficient transport barrier for water. The simple view that only the quantity of suberin present is important, may not hold. A more detailed consideration of both the chemical nature of suberins and of the microstructure of deposits is required, i.e. how suberins impregnate wall pores. PMID:15809280

  20. CV-Dust: Atmospheric aerosol in the Cape Verde region: carbon and soluble fractions of PM10

    NASA Astrophysics Data System (ADS)

    Pio, C.; Nunes, T.; Cardoso, J.; Caseiro, A.; Custódio, D.; Cerqueira, M.; Patoilo, D.; Almeida, S. M.; Freitas, M. C.

    2012-04-01

    Every year, billions of tons of eroded mineral soils from the Saharan Desert and the Sahel region, the largest dust source in the world, cross Mediterranean towards Europe, western Asia and the tropical North Atlantic Ocean as far as the Caribbean and South America. Many aspects of the direct and indirect effects of dust on climate are not well understood and the bulk and surface chemistry of the mineral dust particles determines interactions with gaseous and other particle species. The quantification of the magnitude of warming or cooling remains open because of the strong variability of the atmospheric dust burden and the lack of representative data for the spatial and temporal distribution of the dust composition. CV-Dust is a project that aims at provide a detailed data on the size distribution and the size-resolved chemical and mineralogical composition of dust emitted from North Africa using a natural laboratory like Cape Verde. This archipelago is located in an area of massive dust transport from land to ocean, and is thus ideal to set up sampling devices that are able to characterize and quantify dust transported from Africa. Moreover, Cape Verde's future economic prospects depend heavily on the encouragement of tourism, therefore it is essential to elucidate the role of Saharan dust may play in the degradation of Cape Verde air quality. The main objectives of CV-Dust project are: 1) to characterize the chemical and mineralogical composition of dust transported from Africa by setting up an orchestra of aerosol sampling devices in the strategic archipelago of Cape Verde; 2) to identify the sources of particles in Cape Verde by using receptor models; 3) to elucidate the role Saharan dust may play in the degradation of Cape Verde air quality; 4) to model processes governing dust production, transport, interaction with the radiation field and removal from the atmosphere. Here we present part of the data obtained throughout the last year, involving a set of more than 100 PM10 samples, addressing mainly their mass concentrations and the chemical composition of water soluble ions and carbon species (carbonates and organic and elemental carbon). Different PM10 samplers worked simultaneously in order to collect enough mass to make the aerosol characterization through the different methodologies and to collect aerosols in different filter matrixes, which have to be appropriated to the chemical and mineralogical analysis. The sampling site was located at Santiago Island, in the surroundings of Praia City (14° 55' N e 23° 29' W, 98 m at sea level). High concentrations, up to more than 400 ?g m-3, are connected to north-east and north-northeast winds, and it was identified several dust events characteristic of "bruma seca", whose duration is on average of two to four days. Backward trajectories analysis confirms that the high concentrations in Cape Verde are associated with air masses passing over the Sahara. During dust events the percentage of inorganic water soluble ions for the total PM10 mass concentration decreased significantly to values lower than 10% in comparison with remainder data that range around 45±10%. Acknowledgement: This work was funded by the Portuguese Science Foundation (FCT) through the project PTDD/AAC-CLI/100331/2008 and FCOMP-01-0124-FEDER-008646 (CV-Dust). J. Cardoso acknowledges the PhD grant SFRH-BD-6105-2009 from FCT.

  1. Early inhibition of photosynthesis during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14

    SciTech Connect

    Nable, R.O.; Houtz, R.L.; Cheniae, G.M. )

    1988-04-01

    Early physiological effects of developing Mn toxicity in young leaves of burley tobacco (Nicotiana tabacum L. cv KY 14) were examined in glass-house/water cultured plants grown at high (summer) and low (winter) photon flux. Following transfer of plants to solutions containing 1 millimolar Mn{sup 2+}, sequential samplings were made at various times for the following 9 days, during which Mn accumulation by leaves increased rapidly from {approx} 70 on day 0 to {approx} 1700 and {approx} 5000 microgram per gram dry matter after 1 and 9 days, respectively. In plants grown at high photon flux, net photosynthesis declined by {approx} 20 and {approx} 60% after 1 and 9 days, respectively, and the onset of this decline preceded appearance (after 3 to 4 days) of visible foliar symptoms of Mn toxicity. Intercellular CO{sub 2} concentrations and rates of transpiration were not significantly affected. Though the activity of latent or activated polyphenol oxidase increased in parallel with Mn accumulation, neither leaf respiration nor the activity of catalase (EC 1.11.1.6) and peroxidase (EC 1.10.1.7) were greatly affected. These effects from Mn toxicity could not be explained by any changes in protein or chlorophyll abundance. Additionally, they were not a consequence of Mn induced Fe deficiency. Therefore, inhibition of net photosynthesis and enhancement of polyphenol oxidase activity are early indicators of excess Mn accumulation in tobacco leaves.

  2. Recessive Resistance Derived from Tomato cv. Tyking-Limits Drastically the Spread of Tomato Yellow Leaf Curl Virus

    PubMed Central

    Pereira-Carvalho, Rita C.; Díaz-Pendón, Juan A.; Fonseca, Maria Esther N.; Boiteux, Leonardo S.; Fernández-Muñoz, Rafael; Moriones, Enrique; Resende, Renato O.

    2015-01-01

    The tomato yellow leaf curl disease (TYLCD) causes severe damage to tomato (Solanum lycopersicum L.) crops throughout tropical and subtropical regions of the world. TYLCD is associated with a complex of single-stranded circular DNA plant viruses of the genus Begomovirus (family Geminiviridae) transmitted by the whitefy Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae). The tomato inbred line TX 468-RG is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. Tyking F1. A detailed analysis of this germplasm source against tomato yellow leaf curl virus-Israel (TYLCV-IL), a widespread TYLCD-associated virus, showed a significant restriction to systemic virus accumulation even under continuous virus supply. The resistance was effective in limiting the onset of TYLCV-IL in tomato, as significantly lower primary spread of the virus occurred in resistant plants. Also, even if a limited number of resistant plants could result infected, they were less efficient virus sources for secondary spread owing to the impaired TYLCV-IL accumulation. Therefore, the incorporation of this resistance into breeding programs might help TYLCD management by drastically limiting TYLCV-IL spread. PMID:26008699

  3. Effect of salicylhydroxamic acid on endosperm strength and embryo growth of Lactuca sativa L. cv Waldmann's Green seeds

    NASA Technical Reports Server (NTRS)

    Brooks, C. A.; Mitchell, C. A.

    1988-01-01

    Salicylhydroxamic acid (SHAM) stimulated germination of photosensitive lettuce (Lactuca sativa L. cv Waldmann's Green) seeds in darkness. To determine whether SHAM acts on the embryo or the endosperm, we investigated separately effects of SHAM on growth potential of isolated embryos as well as on endosperm strength. Embryo growth potential was quantified by incubating decoated embryos in various concentrations of osmoticum and measuring subsequent radicle elongation. Growth potential of embryos isolated from seeds pretreated with 4 millimolar SHAM was equal to that of untreated controls. Rupture strength of endosperm tissue excised from seeds pretreated with SHAM was 33% less than that of controls in the micropylar region. To determine if the embryo must be in contact with the endosperm of SHAM to weaken the endosperm, some endosperms were incubated with SHAM only after dissection from seeds. Rupture strength of SHAM-treated, isolated endosperms in the micropylar region was 25% less than that of untreated controls. There was no difference in rupture strength in the cotyledonary region of endosperm isolated from seeds treated with SHAM in buffer or buffer alone. SHAM therefore stimulates germination not by enhancing embryo growth potential, but by weakening the micropylar region of the endosperm enclosing the embryo.

  4. Safety evaluation of sweet lupine (Lupinus albus cv. Multolupa). II. Nine-month feeding and multigeneration study in rats.

    PubMed

    Ballester, D R; Brunser, O; Saitúa, M T; Egaña, J I; Yáñez, E O; Owen, D F

    1984-01-01

    Sweet lupine Lupinus albus cv. Multolupa) flour containing 41.7% protein and 0.025% lupanine was fed for 9 months to two generations of rats (F1 and F2) at a level that provided 20% dietary protein. The diets were supplemented with 0.2% DL-methionine. The control diet provided the same dietary protein level from defatted soya-bean flour, fish meal and dried skimmed milk. The lupine diet had no effect on the general condition, mortality or behaviour of the animals. The growth rate of males fed sweet lupine was significantly higher than that of the controls. Haematological parameters and tests of liver function were normal in all treatment groups. At autopsy there were no significant changes in the weight of the heart, kidney, spleen, brain and gonads. However, the relative weight of the liver of lupine-fed rats was significantly lower than that in the controls. The histology of the liver, like that of the other organs examined, was normal. The reproduction study did not reveal any adverse effect on fertility or lactation that could be attributed to ingestion of sweet lupine. This investigation did not disclose any deleterious effects through two generations of rats that were fed sweet lupine at a level that provided 20% dietary protein for 9 months. PMID:6537936

  5. Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv.'Tropic Sun').

    PubMed

    Colegate, Steven M; Gardner, Dale R; Joy, Robert J; Betz, Joseph M; Panter, Kip E

    2012-04-11

    Cultivation of Crotalaria juncea L. (Sunn Hemp cv. 'Tropic Sun') is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves, and seeds of 'Tropic Sun' were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems, and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid contents of the roots, stems, and leaves of immature plants were estimated to be 0.05, 0.12, and 0.01% w/w, respectively, whereas seeds were estimated to contain 0.15% w/w. PMID:22429238

  6. Isolation and purification of a novel deca-antifungal peptide from potato (Solanum tuberosum L. cv. Jopung) against Candida albicans.

    PubMed

    Lee, Jong-Kook; Gopal, Ramamourthy; Seo, Chang Ho; Cheong, Hyeonsook; Park, Yoonkyung

    2012-01-01

    In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 ?g/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys(3) and Cys(9) or Cys(10). Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents. PMID:22605963

  7. Expression of a cystatin transgene can confer resistance to root lesion nematodes in Lilium longiflorum cv. 'Nellie White'.

    PubMed

    Vieira, Paulo; Wantoch, Sarah; Lilley, Catherine J; Chitwood, David J; Atkinson, Howard J; Kamo, Kathryn

    2015-06-01

    Lilium longiflorum cv. 'Nellie White' assumes a great economic importance as cut flowers, being one of the most valuable species (annual pot plants value above $20,000,000) in terms of wholesales in the US. The root lesion nematode Pratylenchus penetrans (RLN) constitutes one of the main pests for lily producers due to the significant root damage it causes. Our efforts have focused on the generation of soybean hairy roots (as a transient test model) and stable transgenic lilies overexpressing a modified rice cystatin (Oc-I?D86) transgene and challenged with root lesion nematodes. Lily transformation was achieved by gene gun co-bombardment using both a pBluescript-based vector containing the cystatin gene and pDM307 that contains a bar gene for phosphinothricin selection. Both soybean hairy roots and lilies overexpressing the OcI?D86 transgene exhibited enhanced resistance to RLN infection by means of nematode reduction up to 75 ± 5% on the total number of nematodes. In addition, lily plants overexpressing OcI?D86 displayed an increase of plant mass and better growth performance in comparison to wild-type plants, thereby demonstrating an alternative strategy for increasing the yield and reducing nematode damage to this important floral crop. PMID:25398618

  8. Herbicide treatments alter carotenoid profiles for 14C tracer production from tomato ( Solanum lycopersicum cv. VFNT cherry) cell cultures.

    PubMed

    Engelmann, Nancy J; Rogers, Randy B; Lila, Mary Ann; Erdman, John W

    2009-06-10

    Progress in learning about underlying carotenoid bioactivity mechanisms has been limited because of the lack of commercially available radiolabeled lycopene (LYC), phytoene (PE), and phytofluene (PF). Tomato ( Solanum lycopersicum cv. VFNT cherry) cell cultures have been treated to produce [(14)C]-PE and PF but with relatively low yields. To increase carotenoid production, two bleaching herbicides were administered during the culture incubation, 2-(4-chlorophenyl-thio)triethylamine and norflurazon, separately or in combination to produce varying ratios of PE, PF, and LYC. Treatment with both herbicides resulted in optimal production of all three carotenoids. Subsequently, cultures were incubated in [(14)C]-sucrose-containing media to produce labeled LYC, PE, and PF. Adding [(14)C]-sucrose on day 1 of the 14 day culture incubation cycle to norflurazon-treated cultures led to a small increase in labeling efficiency compared to adding it on day 7. Improved culture conditions efficiently provided sufficient (14)C-carotenoids for future cell culture and animal metabolic tracking studies. PMID:19489618

  9. Nitrogen balance for wheat canopies (Triticum aestivum cv. Veery 10) grown under elevated and ambient CO2 concentrations

    NASA Technical Reports Server (NTRS)

    Smart, D. R.; Ritchie, K.; Bloom, A. J.; Bugbee, B. B.

    1998-01-01

    We examined the hypothesis that elevated CO2 concentration would increase NO3- absorption and assimilation using intact wheat canopies (Triticum aestivum cv. Veery 10). Nitrate consumption, the sum of plant absorption and nitrogen loss, was continuously monitored for 23 d following germination under two CO2 concentrations (360 and 1000 micromol mol-1 CO2) and two root zone NO3- concentrations (100 and 1000 mmol m3 NO3-). The plants were grown at high density (1780 m-2) in a 28 m3 controlled environment chamber using solution culture techniques. Wheat responded to 1000 micromol mol-1 CO2 by increasing carbon allocation to root biomass production. Elevated CO2 also increased root zone NO3- consumption, but most of this increase did not result in higher biomass nitrogen. Rather, nitrogen loss accounted for the greatest part of the difference in NO3- consumption between the elevated and ambient [CO2] treatments. The total amount of NO3(-)-N absorbed by roots or the amount of NO3(-)-N assimilated per unit area did not significantly differ between elevated and ambient [CO2] treatments. Instead, specific leaf organic nitrogen content declined, and NO3- accumulated in canopies growing under 1000 micromol mol-1 CO2. Our results indicated that 1000 micromol mol-1 CO2 diminished NO3- assimilation. If NO3- assimilation were impaired by high [CO2], then this offers an explanation for why organic nitrogen contents are often observed to decline in elevated [CO2] environments.

  10. James Webb Space Telescope (JWST) Integrated Science Instruments Module (ISIM) Cryo-Vacuum (CV) Test at GSFC

    NASA Technical Reports Server (NTRS)

    Yew, Calinda M.

    2014-01-01

    JWST ISIM has entered into its system-level testing program at NASA Goddard Space Flight Center (GSFC). In December 2013, ISIM successfully completed the first in a series of three cryo-vacuum tests, which included two flight science instruments. Since then, there have been full-fledged efforts towards the CV2 test scheduled to finish at the end of 2014. The complexity of the mission has generated challenging requirements that demand highly reliable system performance and capabilities from the Space Environment Simulator (SES) vacuum chamber. In order to satisfy the program requirements, GSFC had to develop unique structural and thermal hardware to test ISIM. Most noteworthy is a helium shroud structure and cooling system built in order to achieve operational temperatures below 20K (-253C). This paper: (1) provides an overview of the integrated mechanical and thermal facility systems required to achieve the objectives of JWST ISIM testing, (2) communicates the performance and challenges of the SES during the first ISIM test, and (3) summarizes the action plan to improve the system prior to the next test.

  11. Slight Fermentation with Lactobacillus fermentium Improves the Taste (Sugar:Acid Ratio) of Citrus (Citrus reticulata cv. chachiensis) Juice.

    PubMed

    Yu, Yuanshan; Xiao, Gengsheng; Xu, Yujuan; Wu, Jijun; Fu, Manqin; Wen, Jing

    2015-11-01

    The aim of this study was to evaluate the hypothesis that fermentation with Lactobacillus fermentium, which can metabolize citric acid, could be applied in improving the taste (sugar:acid ratio) of citrus juice. During fermentation, the strain of L. fermentium can preferentially utilize citric acid of citrus (Citrus reticulata cv. Chachiensis) juice to support the growth without the consumption of sugar. After 6 h of fermentation with L. fermentium at 30 °C, the sugar:acid ratio of citrus juice increased to 22:1 from 12:1, which resulted in that the hedonic scores of sweetness, acidity and overall acceptability of fermented-pasteurized citrus juice were higher than the unfermented-pasteurized citrus juice. Compared with unfermented-pasteurized citrus juice, the ORAC value and total amino acid showed a reduction, and no significant change (P > 0.05) in the L*, a*, b*, total soluble phenolics and ascorbic acid (Vc) content in the fermented-pasteurized citrus juice was observed as compared with unfermented-pasteurized citrus juice. Hence, slight fermentation with L. fermentium can be used for improving the taste (sugar:acid ratio) of citrus juice with the well retaining of quality. PMID:26447635

  12. Characterization of the adaptive response of grapevine (cv. Tempranillo) to UV-B radiation under water deficit conditions.

    PubMed

    Martínez-Lüscher, J; Morales, F; Delrot, S; Sánchez-Díaz, M; Gomès, E; Aguirreolea, J; Pascual, I

    2015-03-01

    This work aims to characterize the physiological response of grapevine (Vitis vinifera L.) cv. Tempranillo to UV-B radiation under water deficit conditions. Grapevine fruit-bearing cuttings were exposed to three levels of supplemental biologically effective UV-B radiation (0, 5.98 and 9.66kJm(-2)day(-1)) and two water regimes (well watered and water deficit), in a factorial design, from fruit-set to maturity under glasshouse-controlled conditions. UV-B induced a transient decrease in net photosynthesis (Anet), actual and maximum potential efficiency of photosystem II, particularly on well watered plants. Methanol extractable UV-B absorbing compounds (MEUVAC) concentration and superoxide dismutase activity increased with UV-B. Water deficit effected decrease in Anet and stomatal conductance, and did not change non-photochemical quenching and the de-epoxidation state of xanthophylls, dark respiration and photorespiration being alternative ways to dissipate the excess of energy. Little interactive effects between UV-B and drought were detected on photosynthesis performance, where the impact of UV-B was overshadowed by the effects of water deficit. Grape berry ripening was strongly delayed when UV-B and water deficit were applied in combination. In summary, deficit irrigation did not modify the adaptive response of grapevine to UV-B, through the accumulation of MEUVAC. However, combined treatments caused additive effects on berry ripening. PMID:25617319

  13. Chemical Composition, In vivo Digestibility and Metabolizable Energy Values of Caramba (Lolium multiflorum cv. caramba) Fresh, Silage and Hay

    PubMed Central

    Özelçam, H.; K?rkp?nar, F.; Tan, K.

    2015-01-01

    The experiment was conducted to determine nutritive values of caramba (Lolium multiflorum cv. caramba) fresh, silage and hay by in vivo and in vitro methods. There was a statistically significant difference (p<0.01) in crude protein content value between fresh caramba (12.83%) and silage (8.91%) and hay (6.35%). According to results of experiment, the crude fiber, neutral detergent fiber, acid detergent fiber (ADF), acid detergent lignin contents of the three forms of caramba varied between 30.22% to 35.06%, 57.41% to 63.70%, 35.32% to 43.29%, and 5.55% to 8.86% respectively. There were no significant differences between the three forms of caramba in digestibility of nutrients and in vivo metabolizable energy (ME) values (p>0.05). However, the highest MECN (ME was estimated using crude nutrients) and MEADF values were found in fresh caramba (p<0.01). As a result, it could be said that, there were no differences between the three forms of caramba in nutrient composition, digestibility and ME value, besides drying and ensiling did not affect digestibility of hay. Consequently, caramba either as fresh, silage or hay is a good alternative source of forage for ruminants. PMID:26323399

  14. A new antifungal protein and a chitinase with prominent macrophage-stimulating activity from seeds of Phaseolus vulgaris cv. pinto.

    PubMed

    Ye, X Y; Ng, T B

    2002-01-18

    From the seeds of the pinto bean (Phaseolus vulgaris cv. pinto), a chitinase and a novel antifungal protein, both with the ability of markedly augmenting nitrite production by murine peritoneal macrophages, were isolated. The antifungal proteins, designated phasein A and phasein B, exhibited molecular weights of 28 and 32 kDa, respectively. Phaseins A and B were adsorbed on Affi-gel blue gel and CM-Sepharose and were eluted as adjacent peaks from CM-Sepharose. Phasein A demonstrated potent antifungal activity toward Fusarium oxysporum and Physalospora piricola. Phasein B was more potent than phasein A toward P. piricola but less potent than phasein A toward F. oxysporum and Rhizoctonia solani. Both antifungal proteins inhibited the activity of HIV-1 reverse transcriptase and translation in a rabbit reticulocyte lysate system, with phasein B being more potent. Nitrite production by mouse macrophages was greatly boosted in the presence of both phaseins A and B, although the effect of phasein A was more prominent. The bioactivities of phaseins were in general potent compared with those of other antifungal proteins. PMID:11785974

  15. High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L. f. cv. Golden Leaves

    PubMed Central

    Takahashi, Shunichi; Tamashiro, Ayumu; Sakihama, Yasuko; Yamamoto, Yasusi; Kawamitsu, Yoshinobu; Yamasaki, Hideo

    2002-01-01

    Background The tropical plant Ficus microcarpa L. f. cv. Golden Leaves (GL) is a high-light sensitive tropical fig tree in which sun-leaves are yellow and shade-leaves are green. We compared the response of photosynthetic activities to strong light between GL and its wild-type (WT, Ficus microcarpa L. f.). Results Field measurements of maximum photosystem II (PSII) efficiency (Fv/Fm) of intact sun-leaves in GL showed that photo synthetic activity was severely photoinhibited during the daytime (Fv/Fm = 0.46) and subsequently recovered in the evening (Fv/Fm = 0.76). In contrast, WT did not show any substantial changes of Fv/Fm values throughout the day (between 0.82 and 0.78). Light dependency of the CO2 assimilation rate in detached shade-leaves of GL showed a response similar to that in WT, suggesting no substantial difference in photosynthetic performance between them. Several indicators of photoinhibition, including declines in PSII reaction center protein (D1) content, Fv/Fm value, and O2 evolution and CO2 assimilation rates, all indicated that GL is much more susceptible to photoinhibition than WT. Kinetics of PAM chlorophyll a fluorescence revealed that nonphotochemical quenching (NPQ) capacity of GL was lower than that of WT. Conclusion We conclude that the photosynthetic apparatus of GL is more highly susceptible to photoinhibition than that of WT. PMID:11926968

  16. Characterizing NAD-dependent alcohol dehydrogenase enzymes of Methylobacterium extorquens and strawberry (Fragaria x ananassa cv. Elsanta).

    PubMed

    Koutsompogeras, Panagiotis; Kyriacou, Adamantini; Zabetakis, Ioannis

    2006-01-11

    The methylotroph Methylobacterium extorquens (strain with CABI registration number IMI 369321), which has been isolated from strawberry (Fragaria x ananassa cv. Elsanta) callus cultures, was grown on a mixture of methanol (0.25% v/v) and 1,2-propanediol (0.75% v/v). The microbial biotransformation of 1,2-propanediol to 2-hydroxypropanal (lactaldehyde) was studied. The bacterial alcohol dehydrogenase (ADH) enzymatic activities were assessed, and the optimum pH for ADH activity was found to be pH 6.0. Enzyme assays were carried out for both the bacterial and the strawberry extracts to define the best substrate specificity. For Methylobacterium extorquens, the best substrates were found to be methanol (Km = 0.78 mM) and 1,2-propanediol (Km = 15.84 mM), whereas for strawberries, 1-propanol (Km = 3.54 mM) and ethanol (Km = 6.66 mM) were the best substrates. A wide variety of metals as well as EDTA were shown to decrease the enzymatic activity. Furthermore, SDS-PAGE experiments showed molecular weights of 45.0 and 24.6 kDa for the alcohol dehydrogenases of Methylobacterium extorquens and Fragaria x ananassa, respectively. PMID:16390205

  17. Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3-year comparison.

    PubMed

    Spitaler, Renate; Winkler, Andrea; Lins, Isabella; Yanar, Sema; Stuppner, Hermann; Zidorn, Christian

    2008-03-01

    In continuation of our studies of altitudinal effects on secondary metabolite profile of flowering heads from taxa of the Asteraceae, we investigated phenolic contents and radical scavenging potential from cultivated plants of Arnica montana cv. ARBO during the growing seasons 2003, 2004, and 2005. By conducting experiments on potted plants, we excluded that differences in phenolic contents from plants grown at different altitudes were related primarily to differences in soil composition at these sites. To assess altitudinal and interseasonal variation, plants of A. montana cultivar ARBO were grown in nine experimental plots at altitudes between 590 and 2,230 m at Mount Patscherkofel near Innsbruck, Austria. In all growing seasons and regardless of the soil the plants were grown in, the proportion of flavonoids with vicinal-free hydroxy groups in ring B to flavonoids lacking this feature, and the total amount of caffeic acid derivatives, significantly increased with elevation. These increases of antioxidant phenolics corresponded to an increase of the radical scavenging potential of extracts from plants grown at different altitudes. The results are discussed in regard to previous studies that suggest that enhanced UV-B radiation and decreased temperatures trigger augmented biosynthesis of UV-absorbing and antioxidant phenolics in higher plants. PMID:18259818

  18. Empiric mathematical model for predicting the content of alpha-acids in hop (Humulus lupulus L.) cv. Aurora.

    PubMed

    Sre?ec, Siniša; Ceh, Barbara; Ciler, Tanja Savi?; Rus, Alenka Ferlež

    2013-12-01

    The aim of this research is to find a simple mathematical model due to sum of effective temperatures and rainfalls from second germination after spring pruning till the technological maturity of hop cones, in order to achieve reliable prognosis of alpha-acids content in hop cv. Aurora. After mathematical analyses of experimental data by Eurequa Formulize 0.96 Beta software 17 equations were offered, and after substituting the values of dependent and independent variables in all equations only one equation was chosen with p?=?0.034 (p<0.05). This equation is not reliable in extremely drought year if crop evapotranspiration ET0 in July is higher than 4.5, primarily because of negative influence on formation and development of hop glandular trichomes. Considering achieved results it is possible to suggest following general equation for alpha-acids accumulation in hop: y?=?[(k 1 w)?-?k 2?-?(k 3 w (2))/x]?÷?(-10)???ET 0 July ???4.5. Where y is alpha acids content in dry matter (%), x?=?sum of effective temperatures and w?=?sum of rainfals, both from second germination after spring pruning till technological maturity of hop cones. Coefficients k 1 , k 2 and k 3 are determined for cultivar Aurora (53.8, 453 and 1.33, respectively). PMID:23596559

  19. Response of different maturity stages of sapota (Manilkara achras Mill.) cv. Kallipatti to in-package ethylene absorbent.

    PubMed

    Bhutia, Wangdup; Pal, R K; Sen, Sangita; Jha, S K

    2011-12-01

    Sapota fruits are highly perishable due to their climacteric nature. The rapid softening of fruits is primarily due to high activity of many oxidative enzymes and liberation of ethylene. Harvest maturity plays a crucial role in deciding the marketability of climacteric fruits in general. Attempt has been made to evaluate the response of ethylene absorbent on variable maturity groups of harvested Sapota cv. Kallipatti with the objective to delay the ripening during transit and extend its marketability during storage at ambient condition (27-32 °C & 65-75% R.H.). Harvested fruits having three different degree of ripeness (as maturity indices viz. mature, half-ripe and ripe) were packed with or without ethylene absorbent sachets (Bioconservación, France) in 10 kg CFB boxes and transported from Dahnu to Delhi covering a distant of approximately 2500 KM by truck on road along with conventional packaging as control. The fruits were evaluated immediately on arrival at Delhi and subsequently during storage for various physical, physiological, biochemical and decay parameters. Mature fruits with ethylene absorbent exhibited maximum delay in ripening, low ethylene liberation, weight loss and high fruit firmness. The response of ethylene absorbent to extend the marketability of ripe fruit was not significant. PMID:23572819

  20. Dehydropyrrolizidine Alkaloids, Including Monoesters with an Unusual Esterifying Acid, from Cultivated Crotalaria juncea (Sunn Hemp cv.’Tropic Sun’)

    PubMed Central

    Colegate, Steven M.; Gardner, Dale R.; Joy, Robert J.; Betz, Joseph M.; Panter, Kip E.

    2012-01-01

    Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves and seeds of ‘Tropic Sun’ were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid content of the roots, stems, leaves of immature plants was estimated to be 0.05, 0.12 and 0.01% w/w respectively while seeds were estimated to contain 0.15% w/w. PMID:22429238

  1. Profiles of phenolic compounds and purine alkaloids during the development of seeds of Theobroma cacao cv. Trinitario.

    PubMed

    Pereira-Caro, Gema; Borges, Gina; Nagai, Chifumi; Jackson, Mel C; Yokota, Takao; Crozier, Alan; Ashihara, Hiroshi

    2013-01-16

    Changes occurring in phenolic compounds and purine alkaloids, during the growth of seeds of cacao (Theobroma cacao) cv. Trinitario, were investigated using HPLC-MS/MS. Extracts of seeds with a fresh weight of 125, 700, 1550, and 2050 mg (stages 1-4, respectively) were analyzed. The phenolic compounds present in highest concentrations in developing and mature seeds (stages 3 and 4) were flavonols and flavan-3-ols. Flavan-3-ols existed as monomers of epicatechin and catechin and as procyanidins. Type B procyanidins were major components and varied from dimers to pentadecamer. Two anthocyanins, cyanidin-3-O-arabinoside and cyanidin-3-O-galactoside, along with the N-phenylpropernoyl-l-amino acids, N-caffeoyl-l-aspartate, N-coumaroyl-l-aspartate, N-coumaroyl-3-hydroxytyrosine (clovamide), and N-coumaroyltyrosine (deoxyclovamide), and the purine alkaloids theobromine and caffeine, were present in stage 3 and 4 seeds. Other purine alkaloids, such as theophylline and additional methylxanthines, did not occur in detectable quantities. Flavan-3-ols were the only components to accumulate in detectable quantities in young seeds at developmental stages 1 and 2. PMID:23215441

  2. 5S program to reduce change-over time on forming department (case study on CV Piranti Works temanggung)

    NASA Astrophysics Data System (ADS)

    Rosiana Dewi, Septika; Setiawan, Budi; P, Susatyo Nugroho W.

    2013-06-01

    Productivity is one aspect that determines the success of a company in the competitive world of business. There are seven main types of activities that do not have value-added in manufacturing processes such as overproduction, waiting time, transportation, excess inventory, unnecessary motion and defects. The whole activity is a waste (waste) that can cause harm to the Company. Therefore, in production activities is important to pay attention so that the objectives of production productivity can be achieved. Problems experienced by CV Piranti Works is a production target is not achieved resulting in a lost sale raises the cost of which can cause harm to the Company. From the analysis conducted major known cause of the problem is the length of time required for changeover. This is supported by the high non-value added activity in the changeover activities. Lean Manufacturing is an approach to make system more efficient by reducing waste. This study refers to the book compiled by Takashi Osada (2004) and several other references. In this research used method 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) for the of forming departement. The purpose of this research is to design a work environment using the 5S method (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) and make arrangement of equipment and working tool cabinet design with TRIZ methods. From these results, is expected to eliminate or reduce of non-value added activity and improved the changeover time so as to meet production targets completion of the company.

  3. Determination of valence and conduction-band discontinuities at the (Ga,In) P/GaAs heterojunction by C-V profiling

    NASA Astrophysics Data System (ADS)

    Rao, M. A.; Caine, E. J.; Kroemer, H.; Long, S. I.; Babic, D. I.

    1987-01-01

    The valence and conduction band discontinuities for the lattice matched (Ga,In)P/GaAs heterojunction have been determined by capacitance-voltage (C-V) profiling. Both p-p and n-n heterojunctions were profiled, in order to obtain separate and independent values for both the valence-band-edge discontinuity (?Ev) and the conduction-band discontinuity (?Ec). The band lineup is found to be of the straddling type with the valence- and conduction-band discontinuities 0.24 and 0.22 eV, respectively, with an estimated accuracy of ±10 meV. Computer reconstruction of the C-V profiles was used to check the consistency of the data. The band offset data indicate that the (Ga,In)P/(Al,Ga)As system should be staggered for a certain range of Al compositions.

  4. Effects of series and parallel resistances on the C-V characteristics of silicon-based metal oxide semiconductor (MOS) devices

    NASA Astrophysics Data System (ADS)

    Omar, Rejaiba; Mohamed, Ben Amar; Adel, Matoussi

    2015-04-01

    This paper investigates the electrical behavior of the Al/SiO2/Si MOS structure. We have used the complex admittance method to develop an analytical model of total capacitance applied to our proposed equivalent circuit. The charge density, surface potential, semiconductor capacitance, flatband and threshold voltages have been determined by resolving the Poisson transport equations. This modeling is used to predict in particular the effects of frequency, parallel and series resistance on the capacitance-voltage characteristic. Results show that the variation of both frequency and parallel resistance causes strong dispersion of the C-V curves in the inversion regime. It also reveals that the series resistance influences the shape of C-V curves essentially in accumulation and inversion modes. A significant decrease of the accumulation capacitance is observed when R s increases in the range 200-50000 ?. The degradation of the C-V magnitude is found to be more pronounced when the series resistance depends on the substrate doping density. When R s varies in the range 100 ?-50 k?, it shows a decrease in the flatband voltage from -1.40 to -1.26 V and an increase in the threshold voltage negatively from -0.28 to -0.74 V, respectively. Good agreement has been observed between simulated and measured C-V curves obtained at high frequency. This study is necessary to control the adverse effects that disrupt the operation of the MOS structure in different regimes and optimizes the efficiency of such electronic device before manufacturing.

  5. Petrography, stable isotope compositions, microRaman spectroscopy, and presolar components of Roberts Massif 04133: A reduced CV3 carbonaceous chondrite

    PubMed Central

    Davidson, Jemma; Schrader, Devin L; Alexander, Conel M O'D; Lauretta, Dante S; Busemann, Henner; Franchi, Ian A; Greenwood, Richard C; Connolly, Harold C; Domanik, Kenneth J; Verchovsky, Alexander

    2014-01-01

    Here, we report the mineralogy, petrography, C-N-O-stable isotope compositions, degree of disorder of organic matter, and abundances of presolar components of the chondrite Roberts Massif (RBT) 04133 using a coordinated, multitechnique approach. The results of this study are inconsistent with its initial classification as a Renazzo-like carbonaceous chondrite, and strongly support RBT 04133 being a brecciated, reduced petrologic type >3.3 Vigarano-like carbonaceous (CV) chondrite. RBT 04133 shows no evidence for aqueous alteration. However, it is mildly thermally altered (up to approximately 440 °C); which is apparent in its whole-rock C and N isotopic compositions, the degree of disorder of C in insoluble organic matter, low presolar grain abundances, minor element compositions of Fe,Ni metal, chromite compositions and morphologies, and the presence of unequilibrated silicates. Sulfides within type I chondrules from RBT 04133 appear to be pre-accretionary (i.e., did not form via aqueous alteration), providing further evidence that some sulfide minerals formed prior to accretion of the CV chondrite parent body. The thin section studied contains two reduced CV3 lithologies, one of which appears to be more thermally metamorphosed, indicating that RBT 04133, like several other CV chondrites, is a breccia and thus experienced impact processing. Linear foliation of chondrules was not observed implying that RBT 04133 did not experience high velocity impacts that could lead to extensive thermal metamorphism. Presolar silicates are still present in RBT 04133, although presolar SiC grain abundances are very low, indicating that the progressive destruction or modification of presolar SiC grains begins before presolar silicate grains are completely unidentifiable. PMID:26640360

  6. Lack of effect of aspirin in primary CV prevention in type 2 diabetic patients with nephropathy: results from 8 years follow-up of NID-2 study.

    PubMed

    Sasso, Ferdinando Carlo; Marfella, Raffaele; Pagano, Antonio; Porta, Giovanni; Signoriello, Giuseppe; Lascar, Nadia; Minutolo, Roberto; Carbonara, Ornella; Persico, Marcello; Piscione, Federico; De Nicola, Luca; Torella, Roberto; Paolisso, Giuseppe

    2015-04-01

    The risk-to-benefit ratio for the use of low dose of aspirin in primary cardiovascular (CV) prevention in patients with diabetes mellitus remains to be clarified. We assessed the effect of aspirin on risk of CV events in type 2 diabetic patients with nephropathy, in order to verify the usefulness of Guidelines in clinical practice. We carried out a prospective multicentric study in 564 patients with type 2 diabetic nephropathy free of CV disease attending outpatient diabetes clinics . A total of 242 patients received antiplatelet treatment with aspirin 100 mg/day (group A), and 322 were not treated with antiplatelet drugs (group B). Primary end point was the occurrence of total major adverse cardio-vascular events (MACE). Secondary end points were the relative occurrence of fatal MACE. The average follow-up was 8 years. Total MACE occurred in 49 patients from group A and in 52 patients from group B. Fatal MACE occurred in 22 patients from group A and in 20 from group B; nonfatal MACE occurred in 27 patients from group A and in 32 patients from group B. Kaplan-Meier analysis did not show a statistically significant difference of cumulative MACE between the two groups. A not statistically significant difference in the incidence of both fatal (p = 0.225) and nonfatal CV events (p = 0.573) between the two groups was observed. These results were confirmed after adjustment for confounders (HR for MACE 1.11, 95 % CI 0.91-1.35). These findings suggest that low dose of aspirin is ineffective in primary prevention for patients with nephropathy. PMID:25109286

  7. The C—V and G/?—V Electrical Characteristics of 60Co ?-Ray Irradiated Al/Si3N4/p-Si (MIS) Structures

    NASA Astrophysics Data System (ADS)

    Zeyrek, S.; Turan, A.; M. Bülbül, M.

    2013-07-01

    The influence of 60Co (?-ray) irradiation on the electrical characteristics of Al/Si3N4/p-Si (MIS) structures is investigated using capacitance-voltage (C—V) and conductance-voltage (G/?—V) measurements. The MIS structures are exposed to a 60Co ? radiation source at a dose of 0.7 kGy/h, with a total dose range of 0-100 kGy. The C—V and G/?—V properties are measured before and after 60Co ?-ray irradiation at 500 kHz and room temperature. It is found that the capacitance and conductance values decrease with the increase in the total dose due to the irradiation-induced defects at the interface. The results also indicate that ? radiation causes an increase in the barrier height ?B, Fermi energy EF and depletion layer width WD. The interface state density (Nss), using the Hill-Coleman method and dependent on radiation dose, is determined from the C—V and G/?—V measurements and decreases with an increase in the radiation dose. The decrease in the interface states can be attributed to the decrease in the recombination centers and the passivation of the Si surface due to the deposition insulator layer (Si3N4). In addition, it is clear that the acceptor concentration NA decreases with increasing radiation dose. The profile of series resistance Rs for various radiation doses is obtained from forward and reverse-biased C—V and G/?—V measurements, and its values decrease with increasing radiation dose, while it increases with increasing voltage in the accumulation region

  8. Application of silver nanoparticles contained in ethanol as a working fluid in an oscillating heat pipe with a check valve (CLOHP/CV): a thermodynamic behaviour study

    NASA Astrophysics Data System (ADS)

    Bhuwakietkumjohn, N.; Parametthanuwat, T.

    2015-09-01

    This research focused on the thermal behaviour of oscillating heat pipe with a check valve (CLOHP/CV). The CLOHP/CV was made from a copper capillary tube with an internal diameter of 2.03 mm, 40 turns, and 2 check valves. The CLOHP/CV had three sections: the evaporator and adiabatic condenser of a length of 50, 100, and 150 mm. The angles of inclinations were 90°, 80°, 60°, 40°, 20° and 0° at normal operating conditions. The two working fluids were an ethanol and silver nanoparticles concentration of 0.5 wt% contained in the ethanol ( NE). The filling ratio was 50 % with respect to the total volume of the tube. The operating temperature ( T w ) was 45, 65, 85, 105, 125 and 150 °C. The results of the study showed that the heat flux increased significantly when the operating temperature increased, and the heat flux increased when the L e decreased from 150 to 50 mm. Furthermore, the thermal behaviour of CLOHP/CV showed the highest heat flux of 2,012.63 W/m2 at L e of 50, with an angle of inclination of 90° and operating temperature of 150 °C with NE. The behaviour of 0.5 wt% of silver nanoparticles contained in the ethanol produced a good contact angle. The 0.5 wt% of silver nanoparticles decreased the wet ability, thus increasing the thermal behaviour. The optimum concentration for the addition of silver nanoparticles in the working fluid was 0.5 wt%.

  9. Effects of Different Local Moxibustion-Like Stimuli at Zusanli (ST36) and Zhongwan (CV12) on Gastric Motility and Its Underlying Receptor Mechanism

    PubMed Central

    Su, Yang-Shuai; Xin, Juan-Juan; Yang, Zhao-Kun; He, Wei; Shi, Hong; Wang, Xiao-Yu; Hu, Ling; Jing, Xiang-Hong; Zhu, Bing

    2015-01-01

    The aim of this study was to explore the “intensity-response” relationship in local moxibustion-like stimuli- (LMS-) modulated gastric motility and its underlying receptor mechanism. Based on the thermal pain threshold (43°C), 41°C, 43°C, and 45°C LMS were separately applied to ST36 or CV12 for 180?s among ASIC3 knockout (ASIC3?/?) mice, TRPV1 knockout (TRPV1?/?) mice, and their homologous wild-type C57BL/6 mice (n = 8 in each group). Gastric motility was continuously measured by an intrapyloric balloon, and the amplitude, integral, and frequency of gastric motility during LMS were compared with those of initial activities. We found that both 43°C and 45°C LMS at ST36 induced significantly facilitated effect of gastric motility (P < 0.05), while LMS at CV12 induced inhibited effects (P < 0.05). 41°C LMS had no significant impact on gastric motility. Compared with C57BL/6 mice, the facilitatory effect at ST36 and inhibitive effect of LMS at CV12 were decreased significantly in TRPV1?/? mice (P < 0.05; P < 0.01) but not changed markedly in ASIC3?/? mice (P > 0.05). These results suggest that there existed an “intensity-response” relationship between temperature in LMS and its effects on gastric motility. TRPV1 receptor played a crucial role in the LMS-modulated gastric motility. PMID:26246837

  10. Absence of Dp71 in mdx3cv mouse spermatozoa alters flagellar morphology and the distribution of ion channels and nNOS.

    PubMed

    Hernández-González, Enrique O; Mornet, Dominique; Rendon, Alvaro; Martínez-Rojas, Dalila

    2005-01-01

    In muscle, the absence of dystrophin alters the dystrophin-associated protein complex (DAPC), which is involved in the clustering and anchoring of signaling proteins and ion and water channels. Here we show that mice spermatozoa express only dystrophin Dp71 and utrophin Up71. The purpose of this study was to explore the effect of the absence of Dp71 on the morphology and membrane distribution of members of the DAPC, ion channels and signaling proteins of spermatozoa obtained from dystrophic mutant mdx3cv mice. Our work indicates that although the absence of Dp71 results in a dramatic decrease in beta-dystroglycan, it induces membrane redistribution and an increase in the total level of alpha-syntrophin, voltage-dependent Na+ (micro1) and K+ (Kv1.1) channels and neural nitric oxide synthase (nNOS). The short utrophin (Up71) was upregulated and redistributed in the spermatozoa of mdx3cv mice. A significant increase in abnormal flagella morphology was observed in the absence of Dp71, which was partially corrected when the plasma membrane was eliminated by detergent treatment. Our observations point to a new phenotype associated with the absence of Dp71. Abnormal flagellar structure and altered distribution of ion channels and signaling proteins may be responsible for the fertility problems of mdx3cv mice. PMID:15601658

  11. A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata.

    PubMed

    Camps, Céline; Kappel, Christian; Lecomte, Pascal; Léon, Céline; Gomès, Eric; Coutos-Thévenot, Pierre; Delrot, Serge

    2010-06-01

    Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays comprising 14,500 probes were used to compare, under three experimental conditions (in vitro, in the greenhouse, and in the vineyard), foliar material of infected symptomatic plants (S(+)R(+)), infected asymptomatic plants (S(-)R(+)), and healthy plants (S(-)R(-)). These plants were characterized by symptom notation after natural (vineyard) or experimental (in vitro and greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative real-time PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (Erysiphe necator, Plasmopara viticola, and Botrytis cinerea). (i) Five functional categories of genes, that is those involved in metabolism, defence reactions, interaction with the environment, transport, and transcription, were up-regulated in S(+)R(+) plants compared with S(-)R(-) plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also up-regulated after infection by powdery mildew, downy mildew, and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and the polypeptide fraction secreted by Eutypa. PMID:20190040

  12. Photosynthesis and growth responses of mustard (Brassica juncea L. cv Pusa Bold) plants to free air carbon dioxide enrichment (FACE).

    PubMed

    Ruhil, Kamal; Sheeba; Ahmad, Altaf; Iqbal, Muhammad; Tripathy, Baishnab C

    2015-07-01

    Increased atmospheric [CO2] is likely to affect photosynthesis, plant growth, and yield potential of plants. Mustard (Brassica juncea L.) is an important oil seed crop that is widely grown in India. Therefore, the impact of elevated [CO2] (585 ?mol mol(-1)) on pigment and protein content, chlorophyll a fluorescence, photosynthetic electron transport reactions, CO2 assimilation, biomass production, and seed yield potential was measured in B. juncea cv Pusa Bold, grown inside free air carbon dioxide enrichment (FACE) rings installed on the campus of Jawaharlal Nehru University, New Delhi, India. Plants were grown for three consecutive winter seasons (2010-2013), in ambient (385 ?mol mol(-1)) or elevated [CO2], in field conditions. Elevated [CO2] had no significant effect on the minimal chlorophyll fluorescence (F 0), while the quantum efficiency of Photosystem II, measured as variable fluorescence (F v?=?F m-F 0) to maximum fluoresence (F m), increased by 3 %. Electron transport rate, photosystem I, photosystem II, and whole chain electron transport rates increased by 8 % in elevated [CO2]. However, the net photosynthesis rate increased by ?50 % in three growing seasons under elevated [CO2] condition. The stomatal conductance and transpiration rate decreased resulting in higher photosynthetic water use efficiency. The photosynthesizing surface, i.e., leaf area index substantially increased leading to higher biomass and seed yield under elevated [CO2] condition. Acclimatory downregulation of photosynthesis and plant productivity was not observed in three consecutive growing years suggesting that in the absence of nutrient limitation, B. juncea is highly responsive to elevated CO2 whose yield potential shall increase in changing climatic conditions. PMID:25471475

  13. Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening

    PubMed Central

    Dautt-Castro, Mitzuko; Ochoa-Leyva, Adrian; Contreras-Vergara, Carmen A.; Pacheco-Sanchez, Magda A.; Casas-Flores, Sergio; Sanchez-Flores, Alejandro; Kuhn, David N.; Islas-Osuna, Maria A.

    2015-01-01

    Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. “Kent” was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ? 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like “cell wall,” “carbohydrate catabolic process” and “starch and sucrose metabolic process” among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening. PMID:25741352

  14. FANTINA: Fathom Asteroids Now: Tomography and Imagery of a NEA- Payload For Marco Polo R CV3 / ESA mission

    NASA Astrophysics Data System (ADS)

    Herique, A.; Biele, J.; Bousquet, P.; Ciarletti, V.; Ho, T. M.; Issler, J. L.; Kofman, W.; Michel, P.; Plettemeier, D.; Puget, P.; Souyris, J. C.; Ulamec, S.; van Zoest, T.; Zine, S.

    2012-04-01

    The internal structure of small bodies is still poorly known and has never been measured directly. There is no way to determine from ground based observation whether the body is a monolithic piece of rock or a rubble-pile, an aggregate of boulders held together by gravity and how much porosity it contains, both in the form of micro-scale or macro-scale porosity. Knowing this structure is a key point for a better understanding of the asteroid accretion and dynamical evolution. It is the main objective of the FANTINA experience proposed Payload for Marco Polo R CV3 / ESA mission: FANTINA (Marco Polo's Daughter) for Fathom Asteroids Now: Tomography and Imagery of a NEA is to provide measurement capabilities and science data which are not accessible by remote sensing of the asteroid from the Marco-Polo R spacecraft alone and which complement the analysis of the returned samples. The FANTINA payload is a Lander carrying the ASSERT experiment and a complementary payload to be defined: ASSERT is a bistatic CONSERT/Rosetta-like radar to achieve the tomography both in transmission and in reflexion of the asteroid in order to characterise its composition and its heterogeneity from decimetric to global scale. The lander is a long-lived bus in the 10-kg class derived from MASCOT/Hayabusa 2. This paper reviews the science rationale of FANTINA in the context of the Marco Polo R mission to 1996FG3. The surface package concept will be reviewed including the radar tomography principles. So a preliminary design and budget will be done.

  15. Antioxidant activity and chemical components as potential anticancer agents in the olive leaf (Olea europaea L. cv Leccino.) decoction.

    PubMed

    De Marino, Simona; Festa, Carmen; Zollo, Franco; Nini, Antonella; Antenucci, Lina; Raimo, Gennaro; Iorizzi, Maria

    2014-01-01

    Epidemiological studies have shown that a reduced risk of chronic diseases such as cancer and cardiovascular diseases is correlated with a regular consumption of fruits and vegetable, many of which are rich in polyphenols. The additive and synergistic effect of phytochemicals in fruits and vegetables may reduce chronic diseases related to oxidative stress in human body. Olea europaea L. leaf are rich in phenolic components, which have been proposed to play a role in cancer prevention. The purpose of this study was to identify the main components in the Olea europaea L. leaf (cv. Leccino) preserved during the decoction preparation, in order to delineate the antioxidant activities of the crude extracts and its isolated compounds by using different in vitro assays including DPPH radicalscavenging capacity, total antioxidant capacity (TAC), xanthine oxidase (XO) inhibitory effect and the ability to delay the linoleic acid peroxidation process (ALP). The aqueous decoction was partitioned obtaining four extracts and the n-butanol extract showed the highest antioxidant activity and the highest total phenolic content. Phytochemical investigation leads to the isolation of thirteen secondary metabolites including simple phenolics, flavonoids, secoiridoids whose structures were elucidated by spectroscopic data (1D and 2D NMR) and spectrometric techniques. A significant free radical scavenging effect against DPPH has been evidenced in fraxamoside (1) (EC50 62.6 µM) and taxifolin (5) (EC50 50.0 µM), isolated for the first time from the water decoction. The most active compound in the TAC evaluation, was the 3,4 dihydro-phenyl glycol (8) (0.90 caffeic acid equiv.) while taxifolin and fraxamoside resulted as the most efficient inhibitors of XO activity (IC50 2.7 and 5.2 µM, respectively). Secoxyloganin (4), oleuropein (2) and tyrosol (6) showed the highest ALP activity. This study adds to the growing body of data supporting the bioactivities of phytochemicals and their potential impact on human health. PMID:25102361

  16. Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)

    SciTech Connect

    Lynch, D.V.; Steponkus, P.L. )

    1987-01-01

    Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the plasma membrane, accounting for 16 mole % of the total lipid. Phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine with lesser amounts of phosphatidylglycerol, phosphatidic acid, phosphatidylserine, and phosphatidylinositol, comprised only 32 mole% of the total lipid in NA samples. Following cold acclimation, free sterols increased from 33 to 44 mole %, while steryl glucosides and acylated steryl glucosides decreased from 15 to 6 mole % and 4 to 1 mole %, respectively. Sterol analyses of these lipid classes demonstrated that free {beta}-sitosterol increased from 21 to 32 mole % (accounting for the increase in free sterols as a class) at the expense of sterol derivatives containing {beta}-sitosterol. Glucocerebrosides decreased from 16 to 7 mole % of the total lipid following cold acclimation. In addition, the relative proportions of associated hydroxy fatty acids, including 22:0 (h), 24:0 (h), 22:1 (h), and 24:1 (h) were altered. The phospholipid content of the plasma membrane fraction increased to 42 mole % of the total lipid following cold acclimation. Although the relative proportions of the individual phospholipids did not change appreciably after cold acclimation, there were substantial differences in the molecular species. Di-unsaturated molecular species of phosphatidylcholine and phosphatidylethanolamine increased following acclimation. These results demonstrate that cold acclimation results in substantial changes in the lipid composition of the plasma membrane.

  17. A Lectin with Highly Potent Inhibitory Activity toward Breast Cancer Cells from Edible Tubers of Dioscorea opposita cv. Nagaimo

    PubMed Central

    Chan, Yau Sang; Ng, Tzi Bun

    2013-01-01

    A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC50 values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

  18. Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency

    PubMed Central

    He, Y.; Jones, H. D.; Chen, S.; Chen, X. M.; Wang, D. W.; Li, K. X.; Wang, D. S.; Xia, L. Q.

    2010-01-01

    An efficient Agrobacterium-mediated durum wheat transformation system has been developed for the production of 121 independent transgenic lines. This improved system used Agrobacterium strain AGL1 containing the superbinary pGreen/pSoup vector system and durum wheat cv Stewart as the recipient plant. Acetosyringone at 400??M was added to both the inoculation and cultivation medium, and picloram at 10?mg l?1 and 2?mg l?1 was used in the cultivation and induction medium, respectively. Compared with 200??M in the inoculation and cultivation media, the increased acetosyringone concentration led to significantly higher GUS (?-glucuronidase) transient expression and T-DNA delivery efficiency. However, no evident effects of acetosyringone concentration on regeneration frequency were observed. The higher acetosyringone concentration led to an improvement in average final transformation efficiency from 4.7% to 6.3%. Furthermore, the concentration of picloram in the co-cultivation medium had significant effects on callus induction and regeneration. Compared with 2?mg l?1 picloram in the co-cultivation medium, increasing the concentration to 10?mg l?1 picloram resulted in improved final transformation frequency from 2.8% to 6.3%, with the highest frequency of 12.3% reached in one particular experiment, although statistical analysis showed that this difference in final transformation efficiency had a low level of significance. Stable integration of foreign genes, their expression, and inheritance were confirmed by Southern blot analyses, GUS assay, and genetic analysis. Analysis of T1 progeny showed that, of the 31 transgenic lines randomly selected, nearly one-third had a segregation ratio of 3:1, while the remainder had ratios typical of two or three independently segregating loci. PMID:20202997

  19. Genome organisation and retrotransposon driven molecular evolution of the endosperm Hardness (Ha) locus in Triticum aestivum cv Glenlea.

    PubMed

    Ragupathy, Raja; Cloutier, Sylvie

    2008-12-01

    Wheat endosperm texture is controlled primarily by a locus (Ha), which comprises Gsp-1, Pina and Pinb genes encoding the so-called grain softness protein, puroindoline-a and puroindoline-b, respectively. Pina and Pinb were detected only on the D-genome of hexaploid wheat and its diploid progenitors while Gsp-1 was on all three homoeologous loci. Hexaploid cultivar Glenlea has a hard phenotype due to a null Pina genotype (D-genome) but the sequence organization is not reported. This study aimed at understanding the evolution of homoeologous Ha loci. Sequencing of three BAC clones from cv Glenlea was performed and sequence analyses delimited the Ha loci which spanned 3,925, 5,330 and 31,607 bp in the A-, B- and D-genomes, respectively. A solo LTR of Angela retroelement, downstream to Gsp-A1 and a fragment of Sabrina retroelement, downstream of Gsp-B1, were discovered. We propose that the insertion of these elements into the intergenic regions have driven the deletions of genomic segments harbouring Pina and Pinb genes in the A- and B-genomes of hexaploid wheat. Similarly, fragments of Romani and Vagabond retroelements were identified between truncated Pina and Pinb genes, indicating their role in the deletion of Pina in Glenlea, leading to its hard texture. Structural differences of the Ha locus region of the A-genome between two hexaploid wheat varieties namely Glenlea and Renan (CR626929), suggested the presence of more than one tetraploid ancestor in the origin of hexaploid wheat. PMID:18830712

  20. Modeling the effect of time and temperature on respiration rate of pomegranate arils (cv. "Acco" and "Herskawitz").

    PubMed

    Caleb, Oluwafemi J; Mahajan, Pramod V; Opara, Umezuruike Linus; Witthuhn, Corli R

    2012-04-01

    Understanding the effect of time and temperature on the respiration rate (RR) of fresh-cut produce, towards the design of a suitable modified atmosphere packaging (MAP) system, requires an adequate mathematical model for prediction of RR as a function of both time and temperature. This study investigated the effect of temperature (5, 10, and 15 °C) and storage time (1 to 5 d) on the RR (R(O2) and R(CO2)) of 2 pomegranate cultivars (cv. "Acco" and "Herskawitz") fresh arils. R(O2) and R(CO2) were 3 to 4 folds significantly higher with increased temperature from 5 to 15 °C and were within the range of 2.51 to 7.59 mL/kg h and 2.72 to 9.01 mL/kg h, respectively, for both cultivars. At 15 °C R(CO2) increased significantly from 8.4 to 25.96 mL/kg h from day 1 to 5, respectively, while at 5 °C R(CO2) changed from 2.9 to 2.05 mL/kg h from day 1 to 5. Temperature had the greatest influence on RR and the interaction of time and temperature also significantly affected R(O2) and R(CO2). The respiratory quotient (RQ) estimated by linear regression was 0.98 at 95% significant level. The dependence of RR on temperature and time was accurately described with a combination of an Arrhenius-type and power equation model for and of fresh pomegranate arils. PMID:22429147

  1. Microbiological and biochemical profile of cv. Conservolea naturally black olives during controlled fermentation with selected strains of lactic acid bacteria.

    PubMed

    Panagou, Efstathios Z; Schillinger, Ulrich; Franz, Charles M A P; Nychas, George-John E

    2008-04-01

    The effect of controlled fermentation processes on the microbial association and biochemical profile of cv. Conservolea naturally black olives processed by the traditional anaerobic method was studied. The different treatments included (a) inoculation with a commercial starter culture of Lactobacillus pentosus, (b) inoculation with a strain of Lactobacillus plantarum isolated from a fermented cassava product and (c) uninoculated spontaneous process. Microbial growth, pH, titratable acidity, organic acids and volatile compounds were monitored throughout the fermentation. The initial microbiota consisted of Gram-negative bacteria, lactic acid bacteria and yeasts. Inhibition of Gram-negative bacteria was evident in all processes. Both starter cultures were effective in establishing an accelerated fermentation process and reduced the survival period of Gram-negative bacteria by 5 days compared with the spontaneous process, minimizing thus the likelihood of spoilage. Higher acidification of the brines was observed in inoculated processes without any significant difference between the two selected starter cultures (113.5 and 117.6mM for L. plantarum and L. pentosus, respectively). L. pentosus was also determined as the major species present during the whole process of spontaneous olive fermentation. It is characteristic that lactic acid fermentation was also initiated rapidly in the spontaneous process, as the conditions of fermentation, mainly the low salt level (6%, w/v) favored the dominance of lactic acid bacteria over yeasts. Lactic, acetic and propionic were the organic acids detected by HPLC in considerable amounts, whereas citric and malic acids were also present at low levels and degraded completely during the processes. Ethanol, methanol, acetaldehyde, ethyl acetate were the major volatile compounds identified by GC. Their concentrations varied among the different treatments, reflecting varying degrees of microbial activity in the brines. The results obtained from this study could help the Greek table olive industry to improve the existing processing schemes in order to increase product consistency and quality expanding the international market for naturally black olives. PMID:18206777

  2. Control of endogenous bacterial contamination and micropropagation of a traditional table banana (Musa spp. cv. Kanthali) of Bangladesh.

    PubMed

    Titov, Soubir; Bhowmik, Salil Kumar; Alam, Md Sadrul; Uddin, Sarder Nasir

    2007-11-01

    Shoot tips of a traditional table banana (Musa spp. cv. Kanthali) of Bangladesh were evaluated for in vitro propagation. Initial surface sterilization (with 0.1% HgCl2 for 12 minutes) of shoot tips was successful but microbial contamination (mostly bacteria) at the rhizomatous base of the explants was observed within 6-15 days after inoculation which eventually killed 85% of inoculated explants. So, for contamination free culture establishment explants were soaked in two broad spectrum antibiotics namely ampicillin and gentamicin. Cent percent contamination free cultures were established by soaking the explants in 400 mg/L ampicillin or 200 mg/L gentamicin for 1h. Antibiotic treated explants were found to be full contamination free but failed to regenerate after 3 weeks of culture. But some of them absorbed media for up to 2nd subculture and showed swelling of explants and some color changes from pale white to light/deep green. Finally, a few days after 3rd subculture, no growth of explants was observed and all treated explants eventually started to die. Among the untreated alive explants the best medium for single shoot development was MS + 4.0 mg/L BA + 0.5 mg/L KT + 15% CW and average time required for shoot development was 18-21 days. But the regeneration percentage was very low (30%). The best medium for shoot multiplication was MS + 4.0 mg/L BA + 2.0 mg/L IAA + 15% CW and only average 3-4 shoots were formed per shoot. Finally, in vitro proliferated shoots produced roots with maximum frequency (90%) in half strength of MS medium fortified with 0.5 mg/L IBA. PMID:18257234

  3. Kenney cv short

    E-print Network

    Rachael Kenney

    2013-08-21

    Student Teaching Internship, Carroll High School, Dayton, OH. Spring ... Issues in the Undergraduate Mathematics Preparation of Secondary ..... 2013-2016 ... course over the summer 2009 and has been revised to become a course in the.

  4. John Cushman's CV

    E-print Network

    F. Alejandro Bonilla

    1982 - 1985, Associate Professor of Environmental Physics, Purdue University ..... Curry*, J.E. and J.H. Cushman (1998) Structure in confined fluids: phase ..... Cushman, J.H. (1983) Incorporation of the Rhizosphere into Plant Root Models.

  5. Ilex cornuta cv. Burfordii 

    E-print Network

    James R. Manhart

    2011-08-10

    This publication explains how to minimize damage to live oak trees by the oak leaf roller and an associated caterpillar species, which occur throughout Texas but are most damaging in the Hill Country and South Texas....

  6. cv / Graser Curriculum Vitae

    E-print Network

    Schubart, Christoph

    Master of Laws, Harvard Law School, Cambridge/MA. 1996 Second State Exam in law, State of Baden-Württemberg/Germany. 1994 First State Exam in law, State of Baden-Württemberg, Germany. 1993 Diploma in Legal Studies Public Law and Social Policy, Hertie School of Governance, Berlin. DEGREES 2006 "Habilitation" (post

  7. Identification, quantitation and distribution of gibberellins in fruits of Pisum sativum L. cv. Alaska during pod development.

    PubMed

    García-Martinez, J L; Santes, C; Croker, S J; Hedden, P

    1991-04-01

    In addition to the previously-reported gibberellins: GA1; GA8, GA20 and GA29 (García-Martínez et al., 1987, Planta 170, 130-137), GA3 and GA19 were identified by combined gas chromatography-mass spectrometry in pods and ovules of 4-d-old pollinated pea (Pisum sativum cv. Alaska) ovaries. Pods contained additionally GA17, GA81 (2?-hydroxy GA20) and GA29-catabolite. The concentrations of GA1, GA3, GA8, GA19, GA20 and GA29 were higher in the ovules than in the pod, although, with the exception of GA3, the total content of these GAs in the pod exceeded that in the seeds. About 80% of the GA3 content of the ovary was present in the seeds. The concentrations of GA19 and GA20 in pollinated ovaries remained fairly constant for the first 12 ds after an thesis, after which they increased sharply. In contrast, GA1 and GA3 concentrations were maximal at 7 d and 4-6 d, respectively, after anthesis, at about the time of maximum pod growth rate, and declined thereafter. Emasculated ovaries at anthesis contained GA8, GA19 and GA20 at concentrations comparable with pollinated fruit, but they decreased rapidly. Gibberellins a1 and A3 were present in only trace amounts in emasculated ovaries at any stage. Parthenocarpic fruit, produced by decapitating plants immediately above an emasculated flower, or by treating such flowers with 2,4-dichlorophenoxyacetic acid or GA7, contained GA19 and GA20 at similar concentrations to seeded fruit, but very low amounts of GA1 and GA3 Thus, it appears that the presence of fertilised ovules is necessary for the synthesis of these last two GAs. Mature leaves and leaf diffusates contained GA1, GA8, GA19 and GA20 as determined by combined gas chromatography-mass spectrometry using selected ion monitoring. This provides further evidence that vegetative tissues are a possible alternative source of GAs for fruit-set, particularly in decapitated plants. PMID:24193929

  8. Efficacy of Pneumococcal Nontypable Haemophilus influenzae Protein D Conjugate Vaccine (PHiD-CV) in Young Latin American Children: A Double-Blind Randomized Controlled Trial

    PubMed Central

    Tregnaghi, Miguel W.; Sáez-Llorens, Xavier; López, Pio; Abate, Hector; Smith, Enrique; Pósleman, Adriana; Calvo, Arlene; Wong, Digna; Cortes-Barbosa, Carlos; Ceballos, Ana; Tregnaghi, Marcelo; Sierra, Alexandra; Rodriguez, Mirna; Troitiño, Marisol; Carabajal, Carlos; Falaschi, Andrea; Leandro, Ana; Castrejón, Maria Mercedes; Lepetic, Alejandro; Lommel, Patricia; Hausdorff, William P.; Borys, Dorota; Guiñazú, Javier Ruiz; Ortega-Barría, Eduardo; Yarzábal, Juan P.; Schuerman, Lode

    2014-01-01

    Background The relationship between pneumococcal conjugate vaccine–induced antibody responses and protection against community-acquired pneumonia (CAP) and acute otitis media (AOM) is unclear. This study assessed the impact of the ten-valent pneumococcal nontypable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) on these end points. The primary objective was to demonstrate vaccine efficacy (VE) in a per-protocol analysis against likely bacterial CAP (B-CAP: radiologically confirmed CAP with alveolar consolidation/pleural effusion on chest X-ray, or non-alveolar infiltrates and C-reactive protein ? 40 µg/ml); other protocol-specified outcomes were also assessed. Methods and Findings This phase III double-blind randomized controlled study was conducted between 28 June 2007 and 28 July 2011 in Argentine, Panamanian, and Colombian populations with good access to health care. Approximately 24,000 infants received PHiD-CV or hepatitis control vaccine (hepatitis B for primary vaccination, hepatitis A at booster) at 2, 4, 6, and 15–18 mo of age. Interim analysis of the primary end point was planned when 535 first B-CAP episodes, occurring ?2 wk after dose 3, were identified in the per-protocol cohort. After a mean follow-up of 23 mo (PHiD-CV, n?=?10,295; control, n?=?10,201), per-protocol VE was 22.0% (95% CI: 7.7, 34.2; one-sided p?=?0.002) against B-CAP (conclusive for primary objective) and 25.7% (95% CI: 8.4%, 39.6%) against World Health Organization–defined consolidated CAP. Intent-to-treat VE was 18.2% (95% CI: 5.5%, 29.1%) against B-CAP and 23.4% (95% CI: 8.8%, 35.7%) against consolidated CAP. End-of-study per-protocol analyses were performed after a mean follow-up of 28–30 mo for CAP and invasive pneumococcal disease (IPD) (PHiD-CV, n?=?10,211; control, n?=?10,140) and AOM (n?=?3,010 and 2,979, respectively). Per-protocol VE was 16.1% (95% CI: ?1.1%, 30.4%; one-sided p?=?0.032) against clinically confirmed AOM, 67.1% (95% CI: 17.0%, 86.9%) against vaccine serotype clinically confirmed AOM, 100% (95% CI: 74.3%, 100%) against vaccine serotype IPD, and 65.0% (95% CI: 11.1%, 86.2%) against any IPD. Results were consistent between intent-to-treat and per-protocol analyses. Serious adverse events were reported for 21.5% (95% CI: 20.7%, 22.2%) and 22.6% (95% CI: 21.9%, 23.4%) of PHiD-CV and control recipients, respectively. There were 19 deaths (n?=?11,798; 0.16%) in the PHiD-CV group and 26 deaths (n?=?11,799; 0.22%) in the control group. A significant study limitation was the lower than expected number of captured AOM cases. Conclusions Efficacy was demonstrated against a broad range of pneumococcal diseases commonly encountered in young children in clinical practice. Trial registration www.ClinicalTrials.gov NCT00466947 Please see later in the article for the Editors' Summary PMID:24892763

  9. 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV) induces memory B cell responses in healthy Kenyan toddlers

    PubMed Central

    Muema, D M; Nduati, E W; Uyoga, M; Bashraheil, M; Scott, J A G; Hammitt, L L; Urban, B C

    2015-01-01

    Memory B cells are long-lived and could contribute to persistence of humoral immunity by maintaining the plasma-cell pool or making recall responses upon re-exposure to an antigen. We determined the ability of a pneumococcal conjugate vaccine to induce anti-pneumococcal memory B cells. Frequencies of memory B cells against pneumococcal capsular polysaccharides from serotypes 1, 6B, 14, 19F and 23F were determined by cultured B cell enzyme-linked immunospot (ELISPOT) in 35 children aged 12–23 months who received pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). The relationships between plasma antibodies and memory B cell frequencies were also assessed. After two doses of PHiD-CV, the proportion of subjects with detectable memory B cells against pneumococcal capsular polysaccharides increased significantly for serotypes 1 (3–45%; P?CV is capable of inducing memory B cells against some of the component pneumococcal capsular polysaccharides. PMID:25845628

  10. Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish).

    PubMed

    Wang, Zhuo; Jia, Caihong; Li, Jingyang; Huang, Suzhen; Xu, Biyu; Jin, Zhiqiang

    2015-01-01

    Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubens (Foc) is the most serious disease that attacks banana plants. Salicylic acid (SA) can play a key role in plant-microbe interactions. Our study is the first to examine the role of SA in conferring resistance to Foc TR4 in banana (Musa acuminata L. AAA group, cv. Cavendish), which is the greatest commercial importance cultivar in Musa. We used quantitative real-time reverse polymerase chain reaction (qRT-PCR) to analyze the expression profiles of 45 genes related to SA biosynthesis and downstream signaling pathways in a susceptible banana cultivar (cv. Cavendish) and a resistant banana cultivar (cv. Nongke No. 1) inoculated with Foc TR4. The expression of genes involved in SA biosynthesis and downstream signaling pathways was suppressed in a susceptible cultivar and activated in a resistant cultivar. The SA levels in each treatment arm were measured using high-performance liquid chromatography. SA levels were decreased in the susceptible cultivar and increased in the resistant cultivar. Finally, we examined the contribution of exogenous SA to Foc TR4 resistance in susceptible banana plants. The expression of genes involved in SA biosynthesis and signal transduction pathways as well as SA levels were significantly increased. The results suggest that one reason for banana susceptibility to Foc TR4 is that expression of genes involved in SA biosynthesis and SA levels are suppressed and that the induced resistance observed in banana against Foc TR4 might be a case of salicylic acid-dependent systemic acquired resistance. PMID:25277445

  11. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Aqua-FM3_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  12. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM1_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-09-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  13. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM2_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  14. CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Aqua-FM4_Edition1-CV)

    NASA Technical Reports Server (NTRS)

    Wielicki, Bruce A. (Principal Investigator)

    The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2005-03-29] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

  15. Jornadas Tcnicas Forestales y Ambientales FCF, UNaM EEA Montecarlo, INTA EFECTOS DEL PARASITISMO DEL NEMATODO Beddingia (=Deladenus) siricidicola

    E-print Network

    Sirex noctilio EFFECTS OF THE NEMATODE Beddingia (=Deladenus) siricidicola PARASITISM IN ADULTS STAGE OF WOOD WASP Sirex noctilio Edgar R. Eskiviski 1 Gabriela Faraldo 2 Olga del R. de Coll 3 1 - Ing. Ftal. E@ceel.com.ar SUMMARY The nematode Beddingia siricidicola is the most important agent for the biological control

  16. S-asteroids 387 Aquitania and 980 Anacostia - Possible fragments of the breakup of a spinel-bearing parent body with CO3/CV3 affinities

    NASA Technical Reports Server (NTRS)

    Burbine, Thomas H.; Gaffey, Michael J.; Bell, Jeffrey F.

    1992-01-01

    Asteroids 387 Aquitania and 980 Anacostia are anomalous members of the S-class. Their reflectance spectra exhibit a strong broad absorption feature longwards of 1.5 micron and no significant feature near 1 micron. Their spectra indicate the presence of spinel, an aluminum-magnesium oxide mineral commonly present in inclusions in CV3 and CO3 meteorites. Spinel probably makes up only a small percentage of the surface assemblages of these asteroids, but its spectral effect may be enhanced by its presence in fine-grained white inclusions in immature asteroid regoliths. It is speculated that Aquitania and Anacostia represent material formed in the same nebular zone as the CV3 and CO3 chondrites but either: (A) at an earlier time in the nebula when such inclusions might have been a relatively larger fraction of the nebular grain population; or (B) in local regions where nebular processes (e.g., settling to the midplane) had concentrated such inclusions. The close similarity of two orbital elements (a, i) suggests that Aquitania and Anacostia may be members of a partially dispersed asteroid family produced by the early disruption of a spinel-bearing parent body.

  17. Temperature dependence of frequency dispersion in III-V metal-oxide-semiconductor C-V and the capture/emission process of border traps

    NASA Astrophysics Data System (ADS)

    Vais, Abhitosh; Lin, Han-Chung; Dou, Chunmeng; Martens, Koen; Ivanov, Tsvetan; Xie, Qi; Tang, Fu; Givens, Michael; Maes, Jan; Collaert, Nadine; Raskin, Jean-Pierre; DeMeyer, Kristin; Thean, Aaron

    2015-08-01

    This paper presents a detailed investigation of the temperature dependence of frequency dispersion observed in capacitance-voltage (C-V) measurements of III-V metal-oxide-semiconductor (MOS) devices. The dispersion in the accumulation region of the capacitance data is found to change from 4%-9% (per decade frequency) to ˜0% when the temperature is reduced from 300 K to 4 K in a wide range of MOS capacitors with different gate dielectrics and III-V substrates. We show that such significant temperature dependence of C-V frequency dispersion cannot be due to the temperature dependence of channel electrostatics, i.e., carrier density and surface potential. We also show that the temperature dependence of frequency dispersion, and hence, the capture/emission process of border traps can be modeled by a combination of tunneling and a "temperature-activated" process described by a non-radiative multi-phonon model, instead of a widely believed single-step elastic tunneling process.

  18. Chemical composition, aroma evaluation, and oxygen radical absorbance capacity of volatile oil extracted from Brassica rapa cv. "yukina" used in Japanese traditional food.

    PubMed

    Usami, Atsushi; Motooka, Ryota; Takagi, Ayumi; Nakahashi, Hiroshi; Okuno, Yoshiharu; Miyazawa, Mitsuo

    2014-01-01

    The chemical composition of the volatile oil extracted from the aerial parts of Brassica rapa cv. "yukina" was analyzed using GC-MS, GC-PFPD, and GC-O. A total of 50 compounds were identified. The most prominent constituents were (E)-1,5-heptadiene (40.27%), 3-methyl-3-butenenitrile (25.97%) and 3-phenylpropanenitrile (12.41%). With regard to aroma compounds, 12 compounds were identified by GC-O analysis. The main aroma-active compounds were dimethyl tetrasulfide (sulphury-cabbage, FD = 64), 3-phenylpropanenitrile (nutty, FD = 64), 3-methylindole (pungent, FD = 64), and methional (potato, FD = 32). The antioxidant activity of the aroma-active compounds of the oil was determined using an oxygen radical absorbance capacity (ORAC) assay using fluorescein as the fluorescent probe. The ORAC values were found to be 785 ± 67 trolox equivalents (?mol TE/g) for B. rapa cv. "yukina" oil. The results obtained showed that the volatile oil extracted from the aerial parts is a good dietary source of antioxidants. PMID:24919480

  19. Antisense suppression of thioredoxin h mRNA in Brassica napus cv. Westar pistils causes a low level constitutive pollen rejection response.

    PubMed

    Haffani, Yosr Z; Gaude, Thierry; Cock, J Mark; Goring, Daphne R

    2004-07-01

    In Brassica , the thioredoxin h proteins, THL1 and THL2, were previously found to be potential inhibitors of the S receptor kinase (SRK) in the Brassica self-incompatibility response. To investigate the biological roles of THL1 and THL2 in pollen-pistil interactions, the stigma-specific SLR1 promoter was used to drive antisense THL1/2 expression in Brassica napus cv. Westar. This cultivar is normally compatible, but antisense suppression of THL1/2 led to a low level constitutive rejection of all Brassica napus pollen tested. Fluorescence microscopy revealed that the pollen rejection was a typical Brassica self-incompatibility rejection response with reduced pollen adhesion, germination and pollen tube growth. In addition, Westar was found to express the SLG(15) and SRK(15) proteins which may be the target of regulation by THL1 and THL2. Thus, these results indicate that the THL1 and THL2 are required for full pollen acceptance in B. napus cv. Westar. PMID:15604705

  20. Direct online HPLC-CV-AFS method for traces of methylmercury without derivatisation: a matrix-independent method for urine, sediment and biological tissue samples.

    PubMed

    Brombach, Christoph-Cornelius; Gajdosechova, Zuzana; Chen, Bin; Brownlow, Andrew; Corns, Warren T; Feldmann, Jörg; Krupp, Eva M

    2015-01-01

    Mercury (Hg) is a global pollutant which occurs in different species, with methylmercury (MeHg) being the critical compound due to its neurotoxicity and bioaccumulation through the food chain. Methods for trace speciation of MeHg are therefore needed for a vast range of sample matrices, such as biological tissues, fluids, soils or sediments. We have previously developed an ultra-trace speciation method for methylmercury in water, based on a preconcentration HPLC cold vapour atomic fluorescence spectrometry (HPLC-CV-AFS) method. The focus of this work is mercury speciation in a variety of sample matrices to assess the versatility of the method. Certified reference materials were used where possible, and samples were spiked where reference materials were not available, e.g. human urine. Solid samples were submitted for commonly used digestion or extraction processes to obtain a liquid sample for injection into the analytical system. For MeHg in sediment samples, an extraction procedure was adapted to accommodate MeHg separation from high amounts of Hg(2+) to avoid an overload of the column. The recovery for MeHg determination was found to be in the range of 88-104% in fish reference materials (DOLT-2, DOLT-4, DORM-3), lobster (TORT-2), seaweed (IAEA-140/TM), sediments (ERM(®)-CC580) and spiked urine and has been proven to be robust, reliable, virtually matrix-independent and relatively cost-effective. Applications in the ultra-trace concentration range are possible using the preconcentration up to 200 mL, while for higher MeHg-containing samples, lower volumes can be applied. A comparison was carried out between species-specific isotope dilution gas chromatography inductively coupled plasma mass spectrometry (SSID-GC-ICP-MS) as the gold standard and HPLC-CV-AFS for biological tissues (liver, kidney and muscle of pilot whales), showing a slope of 1.008 and R (2)?=?0.97, which indicates that the HPLC-CV-AFS method achieves well-correlated results for MeHg in biological tissues. PMID:25318461

  1. Plant response to lead in the presence or absence EDTA in two sunflower genotypes (cultivated H. annuus cv. 1114 and interspecific line H. annuus × H. argophyllus).

    PubMed

    Doncheva, Snezhana; Moustakas, Michael; Ananieva, Kalina; Chavdarova, Martina; Gesheva, Emiliya; Vassilevska, Rumyana; Mateev, Plamen

    2013-02-01

    The aim of the present work was to study the response of two sunflower genotypes (cultivated sunflower Helianthus annuus cv. 1114 and newly developed genotype H. annuus × Helianthus argophyllus) to Pb medium-term stress and the role of exogenously applied EDTA in alleviating Pb toxicity in hydroponics. Plant growth, morpho-anatomical characteristics of the leaf tissues, electrolyte leakage, total antioxidant activity, free radical scavenging capacity, total flavonoid content, and superoxide dismutase isoenzyme profile were studied by conventional methods. Differential responses of both genotypes to Pb supplied in the nutrient solution were recorded. Pb treatment induced a decrease in the relative growth rate, disturbance of plasma membrane integrity, and changes in the morpho-anatomical characteristics of the leaf tissues and in the antioxidant capacity, which were more pronounced in the cultivated sunflower H. annuus cv. 1114. The new genotype demonstrated higher tolerance to Pb when compared with the cultivar. This was mainly due to increased photosynthetically active area, maintenance of plasma membrane integrity, permanently high total antioxidant activity, and free radical scavenging capacity as well as total flavonoid content. The addition of EDTA into the nutrient solution led to limitation of the negative impact of Pb ions on the above parameters in both genotypes. This could be related to the reduced content of Pb in the roots, stems, and leaves, suggesting that the presence of EDTA limited the uptake of Pb. The comparative analysis of the responses to Pb treatment showed that the deleterious effect of Pb was more pronounced in the cultivated sunflower H. annuus cv. 1114. The new genotype H. annuus × H. argophyllus was more productive and demonstrated higher tolerance to Pb medium-term stress, which could indicate that it may possess certain mechanisms to tolerate high Pb concentrations. This character could be inherited from the wild parent used in the interspecific hybridization. The ability of EDTA to prevent Pb absorption by the plants could underly the mechanism of limiting of the negative impact of Pb ions. Hence, EDTA cannot be used to enhance Pb absorption from nutrient solution by sunflower plants for phytoremediation purposes. PMID:23135752

  2. Competition for in vitro (/sup 3/H)gibberellin A/sub 4/ binding in cucumber by gibberellins and their derivatives. [Cucumis sativus L. cv National Pickling

    SciTech Connect

    Yalpani, N.; Srivastava, L.M.

    1985-12-01

    The gibberellin (GA) binding properties of a cytosol fraction from hypocotyls of cucumber (Cucumis sativus L. cv National Pickling) were examined using a DEAE filter paper assay, (/sup 3/H)GA/sub 4/, and over 20 GAs, GA derivatives and other growth regulators. The results demonstrate structural specificity of the binding protein for ..gamma..-lactonic C-19 GAs with a 3 ..beta..-hydroxyl and a C-6 carboxyl group. Additional hydroxylations of the A, C, or D ring of the ent-gibberellane skeleton and methylation of the C-6 carboxyl impede or abolish binding affinity. Bioassay data are generally supported by the in vitro results but significantly GA/sub 9/ and GA/sub 36/, both considered to be precursors of GA/sub 4/ in cucumber, show no affinity for the binding protein. The results are discussed in relation to the active site of the putative GA/sub 4/ receptor in cucumber.

  3. Effect of soaking and fermentation on content of phenolic compounds of soybean (Glycine max cv. Merit) and mung beans (Vigna radiata [L] Wilczek).

    PubMed

    María Landete, José; Hernández, Teresa; Robredo, Sergio; Dueñas, Montserrat; de Las Rivas, Blanca; Estrella, Isabel; Muñoz, Rosario

    2015-03-01

    Mung beans (Vigna radiata [L] Wilczek) purchased from a Spanish company as "green soybeans", showed a different phenolic composition than yellow soybeans (Glycine max cv. Merit). Isoflavones were predominant in yellow soybeans, whereas they were completely absent in the green seeds on which flavanones were predominant. In order to enhance their health benefits, both types of bean were subjected to technological processes, such as soaking and fermentation. Soaking increased malonyl glucoside isoflavone extraction in yellow beans and produced an increase in apigenin derivatives in the green beans. Lactobacillus plantarum CECT 748?T fermentation produced an increase in the bioactivity of both beans since a conversion of glycosylated isoflavones into bioactive aglycones and an increase of the bioactive vitexin was observed in yellow and green beans, respectively. In spite of potential consumer confusion, since soybean and "green soybean" are different legumes, the health benefits of both beans were enhanced by lactic fermentation. PMID:25582183

  4. A refractory inclusion in the Kaba CV3 chondrite - Some implications for the origin of spinel-rich objects in chondrites

    NASA Technical Reports Server (NTRS)

    Fegley, B., Jr.; Post, J. E.

    1985-01-01

    The first detailed petrographic and mineralogical study of a Ca, Al-rich inclusion (CAI) from the Kaba CV3 chondrite is reported. This 'fine-grained' CAI contains abundant small, rounded, rimmed, spinel-rich objects which have important features in common with the spinel-rich objects in other carbonaceous and ordinary chondrites. These nodules are interpreted as fractionated distillation residues of primitive dust. However, the available data do not unambiguously rule out a condensation origin for at least some of these objects. Finally, the preservation of distinct diopside-hedenbergite rims on the spinel-rich bodies and the small grain size of many minerals in the CAI matrix material both suggest that the CAI accreted cool and had a relatively cool thermal history in the Kaba parent body.

  5. Optimization of in vitro regeneration and Agrobacterium tumefaciens-mediated transformation with heat-resistant cDNA in Brassica oleracea subsp. italica cv. Green Marvel.

    PubMed

    Ravanfar, Seyed Ali; Aziz, Maheran Abdul; Saud, Halimi Mohd; Abdullah, Janna Ong

    2015-11-01

    An efficient system for shoot regeneration and Agrobacterium tumefaciens-mediated transformation of Brassica oleracea cv. Green Marvel cultivar is described. This study focuses on developing shoot regeneration from hypocotyl explants of broccoli cv. Green Marvel using thidiazuron (TDZ), zeatin, and kinetin, the optimization of factors affecting Agrobacterium-mediated transformation of the hypocotyl explants with heat-resistant cDNA, followed by the confirmation of transgenicity of the regenerants. High shoot regeneration was observed in 0.05-0.1 mg dm(-3) TDZ. TDZ at 0.1 mg dm(-3) produced among the highest percentage of shoot regeneration (96.67 %) and mean number of shoot formation (6.17). The highest percentage (13.33 %) and mean number (0.17) of putative transformant production were on hypocotyl explants subjected to preculture on shoot regeneration medium (SRM) with 200 µM acetosyringone. On optimization of bacterial density and inoculation time, the highest percentage and mean number of putative transformant production were on hypocotyl explants inoculated with a bacterial dilution of 1:5 for 30 min. Polymerase chain reaction (PCR) assay indicated a transformation efficiency of 8.33 %. The luciferase assay showed stable integration of the Arabidopsis thaliana HSP101 (AtHSP101) cDNA in the transgenic broccoli regenerants. Three out of five transgenic lines confirmed through PCR showed positive hybridization bands of the AtHSP101 cDNA through Southern blot analysis. The presence of AtHSP101 transcripts in the three transgenic broccoli lines indicated by reverse transcription-PCR (RT-PCR) confirmed the expression of the gene. In conclusion, an improved regeneration system has been established from hypocotyl explants of broccoli followed by successful transformation with AtHSP101 for resistance to high temperature. PMID:25986972

  6. Polymethoxylated flavones, flavanone glycosides, carotenoids, and antioxidants in different cultivation types of tangerines ( Citrus reticulata Blanco cv. Sainampueng) from Northern Thailand.

    PubMed

    Stuetz, Wolfgang; Prapamontol, Tippawan; Hongsibsong, Surat; Biesalski, Hans-Konrad

    2010-05-26

    Polymethoxylated flavones (PMFs) and flavanone glycosides (FGs) were analyzed in hand-pressed juice and the peeled fruit of 'Sainampueng' tangerines ( Citrus reticulata Blanco cv. Sainampueng) grown in northern Thailand. The tangerines were collected from a citrus cluster of small orchard farmers and were cultivated as either agrochemical-based (AB), agrochemical-safe (AS), or organic grown fruits. Juice samples were also measured on contents of carotenoids, ascorbic acid, and tocopherols. The peel-deriving PMFs tangeretin, nobiletin, and sinensetin were found with high concentrations in juice as a result of simple squeezing, whereas amounts of those PMFs were negligibly low in peeled tangerine fruit. In contrast, the mean concentrations of the FGs narirutin, hesperidin, and didymin were several fold higher in peeled fruit than in tangerine juice and significantly higher in organic than AS and AB tangerines. Narirutin and hesperidin in juice from organic produces as well as narirutin in juice from AS produces were significantly higher than respective mean concentrations in juice from AB produces. beta-Cryptroxanthin was the predominant carotenoid beside zeaxanthin, lutein, lycopene, and beta-carotene in tangerine juice. Ascorbic acid concentrations were not predicted by the type of cultivation, whereas alpha-tocopherol was significantly higher in juice from organic than AS produces. In summary, hand-pressed juice of C. reticulata Blanco cv. Sainampueng serves as a rich source of PMFs, FGs, carotenoids, and antioxidants: 4-5 tangerine fruits ( approximately 80 g of each fruit) giving one glass of 200 mL hand-pressed juice would provide more than 5 mg of nobiletin and tangeretin and 36 mg of hesperidin, narirutin, and didymin, as well as 30 mg of ascorbic acid, >1 mg of provitamin A active beta-cryptoxanthin, and 200 microg of alpha-tocopherol. PMID:20420369

  7. Introducing constricted variational density functional theory in its relaxed self-consistent formulation (RSCF-CV-DFT) as an alternative to adiabatic time dependent density functional theory for studies of charge transfer transitions

    SciTech Connect

    Krykunov, Mykhaylo; Seth, Mike; Ziegler, Tom

    2014-05-14

    We have applied the relaxed and self-consistent extension of constricted variational density functional theory (RSCF-CV-DFT) for the calculation of the lowest charge transfer transitions in the molecular complex X-TCNE between X = benzene and TCNE = tetracyanoethylene. Use was made of functionals with a fixed fraction (?) of Hartree-Fock exchange ranging from ? = 0 to ? = 0.5 as well as functionals with a long range correction (LC) that introduces Hartree-Fock exchange for longer inter-electronic distances. A detailed comparison and analysis is given for each functional between the performance of RSCF-CV-DFT and adiabatic time-dependent density functional theory (TDDFT) within the Tamm-Dancoff approximation. It is shown that in this particular case, all functionals afford the same reasonable agreement with experiment for RSCF-CV-DFT whereas only the LC-functionals afford a fair agreement with experiment using TDDFT. We have in addition calculated the CT transition energy for X-TCNE with X = toluene, o-xylene, and naphthalene employing the same functionals as for X = benzene. It is shown that the calculated charge transfer excitation energies are in as good agreement with experiment as those obtained from highly optimized LC-functionals using adiabatic TDDFT. We finally discuss the relation between the optimization of length separation parameters and orbital relaxation in the RSCF-CV-DFT scheme.

  8. Immunogenicity and safety of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) co-administered with DTPa vaccine in Japanese children: A randomized, controlled study.

    PubMed

    Iwata, Satoshi; Kawamura, Naohisa; Kuroki, Haruo; Tokoeda, Yasunobu; Miyazu, Mitsunobu; Iwai, Asayuki; Oishi, Tomohiro; Sato, Tomohide; Suyama, Akari; François, Nancy; Shafi, Fakrudeen; Ruiz-Guiñazú, Javier; Borys, Dorota

    2015-01-01

    This phase III, randomized, open-label, multicenter study (NCT01027845) conducted in Japan assessed the immunogenicity, safety, and reactogenicity of 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV, given intramuscularly) co-administered with diphtheria-tetanus-acellular pertussis vaccine (DTPa, given subcutaneously). Infants (N=360 ) were randomized (2:1) to receive either PHiD-CV and DTPa (PHiD-CV group) or DTPa alone (control group) as 3-dose primary vaccination (3-4-5 months of age) and booster vaccination (17-19 months of age). Immune responses were measured before and one month after primary/booster vaccination and adverse events (AEs) were recorded. Post-primary immune responses were non-inferior to those in pivotal/efficacy European or Latin American pneumococcal protein D-conjugate vaccine studies. For each PHiD-CV serotype, at least 92.6% of infants post-primary vaccination and at least 97.7% of children post-booster had pneumococcal antibody concentrations ?0.2 ?g/ml, and at least 95.4% post-primary and at least 98.1% post-booster had opsonophagocytic activity (OPA) titers ?8 . Geometric mean antibody concentrations and OPA titers (except OPA titer for 6B) were higher post-booster than post-priming for each serotype. All PHiD-CV-vaccinated children had anti-protein D antibody concentrations ?100 EL.U/ml one month post-primary/booster vaccination and all were seroprotected/seropositive against each DTPa antigen. Redness and irritability were the most common solicited AEs in both groups. Incidences of unsolicited AEs were comparable between groups. Serious AEs were reported for 47 children (28 in PHiD-CV group); none were assessed as vaccine-related. In conclusion, PHiD-CV induced robust immune responses and was well tolerated when co-administered with DTPa in a 3-dose priming plus booster regimen to Japanese children. PMID:25830489

  9. Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): tools for structural and functional genomics

    PubMed Central

    Shultz, JL; Yesudas, C; Yaegashi, S; Afzal, AJ; Kazi, S; Lightfoot, DA

    2006-01-01

    Background The creation of minimally redundant tile paths (hereafter MTP) from contiguous sets of overlapping clones (hereafter contigs) in physical maps is a critical step for structural and functional genomics. Build 4 of the physical map of soybean (Glycine max L. Merr. cv. 'Forrest') showed the 1 Gbp haploid genome was composed of 0.7 Gbp diploid, 0.1 Gbp tetraploid and 0.2 Gbp octoploid regions. Therefore, the size of the unique genome was about 0.8 Gbp. The aim here was to create MTP sub-libraries from the soybean cv. Forrest physical map builds 2 to 4. Results The first MTP, named MTP2, was 14,208 clones (of mean insert size 140 kbp) picked from the 5,597 contigs of build 2. MTP2 was constructed from three BAC libraries (BamHI (B), HindIII (H) and EcoRI (E) inserts). MTP2 encompassed the contigs of build 3 that derived from build 2 by a series of contig merges. MTP2 encompassed 2 Gbp compared to the soybean haploid genome of 1 Gbp and does not distinguish regions by ploidy. The second and third MTPs, called MTP4BH and MTP4E, were each based on build 4. Each was semi-automatically selected from 2,854 contigs. MTP4BH was 4,608 B and H insert clones of mean size 173 kbp in the large (27.6 kbp) T-DNA vector pCLD04541. MTP4BH was suitable for plant transformation and functional genomics. MTP4E was 4,608 BAC clones with large inserts (mean 175 kbp) in the small (7.5 kbp) pECBAC1 vector. MTP4E was suitable for DNA sequencing. MTP4BH and MTP4E clones each encompassed about 0.8 Gbp, the 0.7 Gbp diploid regions and 0.05 Gbp each from the tetraploid and octoploid regions. MTP2 and MTP4BH were used for BAC-end sequencing, EST integration, micro-satellite integration into the physical map and high information content fingerprinting. MTP4E will be used for genome sequence by pooled genomic clone index. Conclusion Each MTP and associated BES will be useful to deconvolute and ultimately finish the whole genome shotgun sequence of soybean. PMID:16725032

  10. Direct purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel using a PEG/salt-based Aqueous Two Phase System.

    PubMed

    Mehrnoush, Amid; Sarker, Md Zaidul Islam; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2011-01-01

    An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000-10,000), potassium phosphate composition (12-20%, w/w), system pH (6-9), and addition of different concentrations of neutral salts (0-8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel. PMID:21986520

  11. Plant growth enhancing effects by a siderophore-producing endophytic streptomycete isolated from a Thai jasmine rice plant (Oryza sativa L. cv. KDML105).

    PubMed

    Rungin, Siriwan; Indananda, Chantra; Suttiviriya, Pavinee; Kruasuwan, Worarat; Jaemsaeng, Ratchaniwan; Thamchaipenet, Arinthip

    2012-10-01

    An endophytic Streptomyces sp. GMKU 3100 isolated from roots of a Thai jasmine rice plant (Oryza sativa L. cv. KDML105) showed the highest siderophore production on CAS agar while phosphate solubilization and IAA production were not detected. A mutant of Streptomyces sp. GMKU 3100 deficient in just one of the plant growth promoting traits, siderophore production, was generated by inactivation of a desD-like gene encoding a key enzyme controlling the final step of siderophore biosynthesis. Pot culture experiments revealed that rice and mungbean plants inoculated with the wild type gave the best enhancement of plant growth and significantly increased root and shoot biomass and lengths compared with untreated controls and siderophore-deficient mutant treatments. Application of the wild type in the presence or absence of ferric citrate significantly promoted plant growth of both plants. The siderophore-deficient mutant clearly showed the effect of this important trait involved in plant-microbe interaction in enhancement of growth in rice and mungbean plants supplied with sequestered iron. Our results highlight the value of a substantial understanding of the relationship of the plant growth promoting properties of endophytic actinomycetes to the plants. Endophytic actinomycetes, therefore, can be applied as potentially safe and environmentally friendly biofertilizers in agriculture. PMID:22836676

  12. Immunodetection and Identification of N6-(o-Hydroxybenzylamino)Purine as a Naturally Occurring Cytokinin in Populus × canadensis Moench cv Robusta Leaves 1

    PubMed Central

    Strnad, Miroslav; Peters, Wilfried; Beck, Erwin; Kamínek, Miroslav

    1992-01-01

    A highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for 9-?-d-ribofuranosyl-N6-(o-hydroxybenzylamino)purine [(oOH)[9R]BAP] and structurally related cytokinins. As little as 3 femtomoles of the compound could be detected by this method. Cross-reactivity studies demonstrated the specificity of four polyclonal antibodies for (oOH)[9R] BAP and its free base in preference to a range of natural cytokinins and other purines. After evaluating the method by internal standardization employing [2-3H](oOH)[9R]BAP of high specific radioactivity as recovery marker by dilution analyses and by immunohistograms, it was possible to apply ELISA to quantify (oOH)[9R]BAP in plant extracts. In addition to (oOH)[9R]BAP, an unknown cytokinin reacting with the same antibody was detected in high performance liquid chromatography-fractionated extracts of mature Populus × canadensis Moench cv Robusta. The structure of the new compound was determined by gas chromatography-mass spectrometry and finally confirmed by synthesis as N6-(o-hydroxybenzylamino)purine. PMID:16668886

  13. Effect of boiling time on chemical composition and physico-functional properties of flours from taro (Colocasia esculenta cv fouê) corm grown in Côte d'Ivoire.

    PubMed

    Amon, Anon Simplice; Soro, René Yadé; Assemand, Emma Fernande; Dué, Edmond Ahipo; Kouamé, Lucien Patrice

    2014-05-01

    Taro (Colocasia esculenta cv fouê) corm was subjected to different boiling times and the changes in chemical composition and physico-functional properties were investigated using standard method. The change in boiling time led to a significant (P?

  14. Relationship of superficial scald development and alpha-farnesene oxidation to reactions of diphenylamine and diphenylamine derivatives in Cv. Granny Smith apple peel.

    PubMed

    Rudell, David R; Mattheis, James P; Fellman, John K

    2005-10-19

    Cv. Granny Smith apple fruit, treated at harvest with aqueous emulsions containing diphenylamine (DPA) and DPA derivatives, were evaluated for the peel disorder superficial scald (scald) after 6 months of cold storage at 1 degrees C plus 0 or 7 days at 20 degrees C. Metabolism of these derivatives and alpha-farnesene oxidation were also evaluated after 6 months. Derivatives substituted at the para position prevented scald, but scald developed on fruit treated with derivatives substituted in the amino, ortho, or meta positions. The extent of scald control was also dependent on the chemical nature of the functional group used to derivatize DPA. Hydroxylation of DPA and DPA derivatives during storage was not associated with scald control. Methoxylated DPA derivatives produced during storage resulted from O-methylation of C-hydroxylated derivatives rather than C-methoxylation of DPA. N-Nitrosodiphenylamine provided partial scald control, possibly resulting from its degradation to DPA, indicating that the amino hydrogen of DPA may be crucial for scald control. Results suggest that functional group position and chemical properties both contribute to the efficacy of DPA derivatives for scald control. PMID:16218691

  15. The sequence of change within the photosynthetic apparatus of wheat following short-term exposure to ozone. [Triticum aestivum L. cv Avalon

    SciTech Connect

    Farage, P.K.; Long, S.P.; Baker, N.R. ); Lechner, E.G. )

    1991-02-01

    The basis of inhibition of photosynthesis by single acute O{sub 3} exposures was investigated in vivo using analyses based on leaf gas exchange measurements. The fully expanded second leaves of wheat plants (Triticum aestivum L. cv Avalon) were fumigated with either 200 or 400 nanomoles per mole O{sub 3} for between 4 and 16 hours. This reduced significantly the light-saturated rate of Co{sub 2} uptake and was accompanied by a parallel decrease in stomatal conductance. However, the stomatal limitation only increased significantly during the first 8 hours of exposure to 400 nanomoles per mole O{sub 3}; no significant increase occurred for any of the other treatments. Analysis of the response of CO{sub 2} uptake to the internal Co{sub 2} concentration implied that the predominant factor responsible for the reduction in light-saturated CO{sub 2} uptake was a decrease in the efficiency of carboxylation. At saturating concentrations of Co{sub 2}, photosynthesis was inhibited by no more than 22% after 16 hours, indicating that the capacity for regeneration of ribulose bisphosphate was less susceptible to O{sub 3}. Ozone fumigations also had a less pronounced effect on light-limited photosynthesis. The photochemical efficiency of photosystem II estimated from the ratio of variable to maximum chlorophyll fluorescence and the atrazine-binding capacity of isolated thylakoids demonstrated that photochemical reactions were not responsible for the initial inhibition of CO{sub 2} uptake.

  16. Breeding for blast-disease-resistant and high-yield Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutants using low-energy ion beams

    NASA Astrophysics Data System (ADS)

    Mahadtanapuk, S.; Teraarusiri, W.; Phanchaisri, B.; Yu, L. D.; Anuntalabhochai, S.

    2013-07-01

    Low-energy ion beam was applied on mutation induction for plant breeding of blast-disease-resistant Thai jasmine rice (Oryza sativa L. cv. KDML 105). Seeds of the wild-type rice were bombarded in vacuum by nitrogen ion beam at energy of 60-80 keV to a beam fluence range of 2 × 1016-2 × 1017 ions/cm2. The ion-bombarded rice seeds were grown in soil for 2 weeks as transplanted rice in plastic pots at 1 seedling/pot. The seedlings were then screened for blast resistance by Pyricularia grisea inoculation with 106 spores/ml concentrations. The blast-resistant rice mutant was planted up to F6 generation with the consistent phenotypic variation. The high percentage of the blast-disease-resistant rice was analyzed with DNA fingerprint. The HAT-RAPD (high annealing temperature-random amplified polymorphic DNA) marker revealed the modified polymorphism fragment presenting in the mutant compared with wild type (KDML 105). The cDNA fingerprints were investigated and the polymorphism fragment was subcloned into pGEM-T easy vector and then sequenced. The sequence of this fragment was compared with those already contained in the database, and the fragment was found to be related to the Spotted leaf protein 11 (Spl11).

  17. Senescence-specific alteration of hydrogen peroxide levels in Arabidopsis thaliana and oilseed rape spring variety Brassica napus L. cv. Mozart.

    PubMed

    Bieker, Stefan; Riester, Lena; Stahl, Mark; Franzaring, Jürgen; Zentgraf, Ulrike

    2012-08-01

    In order to analyze the signaling function of hydrogen peroxide (H(2)O(2)) production in senescence in more detail, we manipulated intracellular H(2)O(2) levels in Arabidopsis thaliala (L.) Heynh by using the hydrogen-peroxide-sensitive part of the Escherichia coli transcription regulator OxyR, which was directed to the cytoplasm as well as into the peroxisomes. H(2)O(2) levels were lowered and senescence was delayed in both transgenic lines, but OxyR was found to be more effective in the cytoplasm. To transfer this knowledge to crop plants, we analyzed oilseed rape plants Brassica napus L. cv. Mozart for H(2)O(2) and its scavenging enzymes catalase (CAT) and ascorbate peroxidase (APX) during leaf and plant development. H(2)O(2) levels were found to increase during bolting and flowering time, but no increase could be observed in the very late stages of senescence. With increasing H(2)O(2) levels, CAT and APX activities declined, so it is likely that similar mechanisms are used in oilseed rape and Arabidopsis to control H(2)O(2) levels. Under elevated CO(2) conditions, oilseed rape senescence was accelerated and coincided with an earlier increase in H(2)O(2) levels, indicating that H(2)O(2) may be one of the signals to inducing senescence in a broader range of Brassicaceae. PMID:22805117

  18. Pathways of nitrogen assimilation in cowpea nodules studied using /sup 15/N/sub 2/ and allopurinol. [Vigna unguiculata L. Walp. cv Vita

    SciTech Connect

    Atkins, C.A.; Storer, P.J.; Pate, J.S.

    1988-01-01

    In the presence of 0.5 millimolar allopurinol (4-hydroxypyrazolo (3,4-d)pyrimidine), an inhibitor of NAD:xanthine oxidoreductase (EC 1.2.3.2), intact attached nodules of cowpea (vigna unguiculata L. Walp. cv Vita 3) formed (/sup 15/N)xanthine from /sup 15/N/sub 2/ at rates equivalent to those of ureide synthesis, confirming the direct assimilation of fixed nitrogen into purines. Xanthine accumulated in nodules and was exported in increasing amounts in xylem of allopurinol-treated plants. Other intermediates of purine oxidation, de novo purine synthesis, and ammonia assimilation did not increase and, over the time course of experiments (4 hours), allopurinol had no effect on nitrogenase (EC 1.87.99.2) activity. Negligible /sup 15/N -labeling of asparagine from /sup 15/N/sub 2/ was observed, suggesting that the significant pool (up to 14 micromoles per gram of nodule fresh weight) of this amide in cowpea nodules was not formed directly from fixation but may have accumulated as a consequence of phloem delivery.

  19. Purification and characterization of a glucosamine-binding antifungal lectin from Phaseolus vulgaris cv. Chinese pinto beans with antiproliferative activity towards nasopharyngeal carcinoma cells.

    PubMed

    Ang, Andrew Si Wo; Cheung, Randy Chi Fai; Dan, Xiuli; Chan, Yau Sang; Pan, Wenliang; Ng, Tzi Bun

    2014-01-01

    A lectin has successfully been isolated from Phaseolus vulgaris cv. Chinese pinto bean using affinity chromatography, ion exchange chromatography, and gel filtration in succession, with a 15.4-fold purification. Investigation of its characteristics revealed that Chinese pinto bean lectin (CPBL) was a 58-kDa dimeric glucosamine-binding protein. Its Mg(2+)-dependent hemagglutinating activity was stable at pH 7-8 and at or below 60 °C. When the purified lectin was tested against six fungal species including Phyllosticta citriasiana, Magnaporthe grisea, Bipolans maydis, Valsa mali, Mycosphaerella arachidicola, and Setosphaeria turcica, only the mycelial growth of V. mali was reduced by 30.6 % by the lectin at 30 ?M. The lectin did not exert any discernible antiproliferative effects on breast cancer MCF-7 cells, but was able to suppress proliferation of nasopharyngeal carcinoma HONE-1 cells, with an IC50 of 17.3 ?M, as revealed by the MTT assay. Since few plant lectins demonstrate antifungal activity against V. mali, and not many others have inhibitory effects on HONE-1 cells, CPBL is a distinctive lectin which may be exploited for development into an agent against V. mali and HONE-1 cells. PMID:24114321

  20. Isolation of a Hemagglutinin with Potent Antiproliferative Activity and a Large Antifungal Defensin from Phaseolus vulgaris cv. Hokkaido Large Pinto Beans.

    PubMed

    Yin, Cuiming; Wong, Jack Ho; Ng, Tzi Bun

    2015-06-10

    Lectins (hemagglutinins) are defined as sugar-binding proteins or glycoproteins with various biological activities. A 60 kDa dimeric hemagglutinin with a blocked N-terminus was isolated in large yield (190 mg/60 g) from the common edible bean Phaseolus vulgaris cv. Hokkaido large pinto bean. Its hemagglutinating, antifungal, and antitumor activities as well as the effects of carbohydrate and metal ions on its hemagglutinating activity were examined. It inhibited the proliferation of nasopharyngeal carcinoma (CNE2), human breast cancer (MCF7), and hepatoma (HepG2) cells. The IC50 values toward HepG2, MCF7, and CNE2 cells after treatment for 48 h were 8.1, 6.07, and 7.49 ?M, respectively, which were relatively low among lectins of different P. vulgaris cultivars. From the pinto beans, a 10888 Da antifungal peptide with similarity to plant defensins as revealed by mass spectroscopic analysis was also isolated with a yield of 3.2 mg of proteins from 60 g of beans. The large defensin was capable of inhibiting mycelial growth in Mycosphaerella arachidicola, Setosphaeria turcica, Bipolaris maydis, and Fusarium oxysporum but not in Valsa mali. PMID:25965006

  1. Effects of modified atmosphere packing and honey dip treatments on quality maintenance of minimally processed grape cv. Razaki (V. vinifera L.) during cold storage.

    PubMed

    Sab?r, Ali; Sab?r, Ferhan K; Kara, Zeki

    2011-06-01

    Increasing pressure in food conservation sector to replace chemical applications has urged researchers to focus on studying new strategies of extending the postharvest life of produces. In such efforts, numerous materials have been tested for their effectiveness as well as suitability in organic consumption. In this study, effects of modified atmosphere packing (MAP) and honey solution dip on maintenance of quality of minimally processed table grape cv. Razaki were investigated. During the storage at 0 °C with relative humidity of 90%, MAP, honey dip, and their combined applications significantly retarded the weight loss of berries that retained about 2 mm of cap stem. Soluble solid contents of all berries slightly increased, while their acid amounts decreased, resulting in consecutive rises of maturity index. With respect to the sensory score, calculated as mean of ten panelists, honey treatment alone was ranked the highest while control berries had significantly lower value. Overall, MAP, honey solution dip or their combination significantly maintained the general quality of minimally processed grape by delaying quality loss and berry decay. Therefore, honey solution dip yielded promising results to use as an edible organic coating barrier to moisture and resist to water vapor diffusion during the cold storage, offering a good adherence to berry surface. PMID:23572752

  2. Developmental patterns of emission of scent compounds and related gene expression in roses of the cultivar Rosa x hybrida cv. 'Yves Piaget'.

    PubMed

    Chen, Xiaomin; Baldermann, Susanne; Cao, Shuyan; Lu, Yao; Liu, Caixia; Hirata, Hiroshi; Watanabe, Naoharu

    2015-02-01

    2-Phenylethanol (2PE) and 3,5-dimethoxytoluene (DMT) are characteristic scent compounds in specific roses such as Rosa x hybrida cv. 'Yves Piaget'. We analyzed the endogenous concentrations and emission of 2PE and DMT during the unfurling process in different floral organs, as well as changes in transcript levels of the two key genes, PAR and OOMT2. The emission of both 2PE and DMT increased during floral development to reach peaks at the fully unfurled stage. The relative transcripts of PAR and OOMT2 also increased during floral development. Whereas the maximum for OOMT2 was found at the fully unfurled stage (stage 4), similar expression levels of PAR were detected at stage 4 and the senescence stage (stage 6). The results demonstrate a positive correlation between the expression levels of PAR and OOMT2 and the emission of 2PE and DMT. In addition, endogenous volatiles and relative transcripts showed tissue- and development-specific patterns. PMID:25576838

  3. Spatial and temporal variability of cv. Tempranillo phenology and grape quality within the Ribera del Duero DO (Spain) and relationships with climate

    NASA Astrophysics Data System (ADS)

    Ramos, M. C.; Jones, G. V.; Yuste, J.

    2015-04-01

    The aim of this work was to analyze spatial phenology and grape quality variability related to the climatic characteristics within the Ribera del Duero Designation of Origin (DO). Twenty plots planted with cv. Tempranillo and distributed within the DO were analyzed for phenology from 2004 to 2013. Grape quality parameters at ripening (berry weight, sugar content, acidity and pH, and anthocyanins) were analyzed in 26 plots for the period 2003-2013. The relationships between phenology and grape parameters with different climatic variables were confirmed with a multivariate analysis. On average, bud break was April 27th, bloom June 17th, and veraison August 12th. However, phenology during the time period showed high variability, with differences between years of up to 21 days for a phenology stage. The earliest dates were observed in dry years (2005, 2006, and to a lesser degree in 2009) while the later phenology dates occurred in the wettest year of the period (2008). High correlations were found between veraison date and temperature variables as well as with precipitation-evapotranspiration recorded during the bloom-veraison period. These effects tended to be higher in the central part of the DO. Grape quality parameters also showed high variability among the dry and the wet years, and the influence of extreme temperatures on color development as well as the effect of available water on acidity were observed.

  4. Spatial and temporal variability of cv. Tempranillo phenology and grape quality within the Ribera del Duero DO (Spain) and relationships with climate.

    PubMed

    Ramos, M C; Jones, G V; Yuste, J

    2015-12-01

    The aim of this work was to analyze spatial phenology and grape quality variability related to the climatic characteristics within the Ribera del Duero Designation of Origin (DO). Twenty plots planted with cv. Tempranillo and distributed within the DO were analyzed for phenology from 2004 to 2013. Grape quality parameters at ripening (berry weight, sugar content, acidity and pH, and anthocyanins) were analyzed in 26 plots for the period 2003-2013. The relationships between phenology and grape parameters with different climatic variables were confirmed with a multivariate analysis. On average, bud break was April 27(th), bloom June 17(th), and veraison August 12th. However, phenology during the time period showed high variability, with differences between years of up to 21 days for a phenology stage. The earliest dates were observed in dry years (2005, 2006, and to a lesser degree in 2009) while the later phenology dates occurred in the wettest year of the period (2008). High correlations were found between veraison date and temperature variables as well as with precipitation-evapotranspiration recorded during the bloom-veraison period. These effects tended to be higher in the central part of the DO. Grape quality parameters also showed high variability among the dry and the wet years, and the influence of extreme temperatures on color development as well as the effect of available water on acidity were observed. PMID:25906047

  5. The photosynthetic and stomatal response of Medicago sativa cv. saranac to free-air CO{sub 2} enrichment (F.A.C.E.) and nitrogen

    SciTech Connect

    Bridson, N.P.

    1996-08-01

    Plots of Medicago sativa cv. saranac were grown in the field at ambient (355 {mu}mol CO{sub 2} mol{sup -1} air) or elevated (600{mu}mol CO{sub 2} mol{sup -1} air) CO{sub 2} concentrations. High (200kg yr{sup -1}) or low (20kg yr{sup -1}) nitrogen levels were applied to two isogeneic lines, one able and one unable to use nitrogen fixing bacteria. Plants were in the second year of field growth. Exposure to elevated CO{sub 2} was via a Free-Air CO{sub 2} Enrichment System (FACE). Elevated CO{sub 2} increased diurnal assimilation by between 12% and 92%. Analysis of A/C{sub i} responses showed that effective nitrogen fertilisation was more important to rubisCO and RuBP activity than elevated CO{sub 2}. No acclimation was consistently observed. Leaves lower down the canopy were found to have lower Vc{sub max} and J{sub max} values, though age may be the cause of the latter effect. FACE conditions have only a small effect on these responses. There was some evidence found for the down-regulation of photosynthesis in the late afternoon. The FACE conditions had no affect on stomatal density but did increase epidermal cell density.

  6. Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv. Heavenly Blue

    PubMed Central

    Yoshida, Kumi; Miki, Naoko; Momonoi, Kazumi; Kawachi, Miki; Katou, Kiyoshi; Okazaki, Yoshiji; Uozumi, Nobuyuki; Maeshima, Masayoshi; Kondo, Tadao

    2009-01-01

    Petal color change in morning glory Ipomoea tricolor cv. Heavenly Blue, from red to blue, during the flower-opening period is due to an unusual increase in vacuolar pH (pHv) from 6.6 to 7.7 in colored epidermal cells. We clarified that this pHv increase is involved in tonoplast-localized Na+/H+ exchanger (NHX). However, the mechanism of pHv increase and the physiological role of NHX1 in petal cells have remained obscure. In this study, synchrony of petal-color change from red to blue, pHv increase, K+ accumulation, and cell expansion growth during flower-opening period were examined with special reference to ItNHX1. We concluded that ItNHX1 exchanges K+, but not Na+, with H+ to accumulate an ionic osmoticum in the vacuole, which is then followed by cell expansion growth. This function may lead to full opening of petals with a characteristic blue color. PMID:19521056

  7. Evidence of Recombinant Citrus tristeza virus Isolate Occurring in Acid Lime cv. Pant Lemon Orchard in Uttarakhand Terai Region of Northern Himalaya in India.

    PubMed

    Singh, Jaywant Kumar; Tarafdar, Avijit; Sharma, Susheel Kumar; Biswas, Kajal Kumar

    2013-06-01

    The present study for the first time describes biological and molecular characterization of Citrus tristeza virus (CTV) occurring in the Terai area of Uttarakhand State in Northern Himalaya region of India. Direct antigen coated-ELISA and reverse transcriptase-polymerase chain reaction (RT-PCR) detected the CTV infection in Acid lime cv. Pant lemon (Citrus aurantifolia) orchards of Pantnagar with an estimated disease incidence of 16.6-20.5 %. To know the biological and genetic properties, an isolate, CTV Pant 4 was characterized. Isolate Pant 4 could be graft transmitted to Kinnow, Nagpur and Darjeeling mandarins, Mosambi sweet orange, Kagzi lime, Sweet lime, Sour orange but not to Rough lemon. The sequence analyses of the 5'ORF1a (3038 nucleotides) of LPro domain and 3'end (2058 nt) covering ORF7-ORF10 regions of the CTV genome revealed that Pant 4 was closely related to the previously reported Indian CTV isolate, Kpg3 from Northeastern Himalaya region with 97 and 98 % sequence identity, respectively. Whereas, it differed from the previously reported CTV isolate B165 from Southern India with 79 and 92 % identity, respectively for 5'ORF1a and 3' end regions. Recombination and SplitsTree decomposition analyses indicated that CTV isolate Pant 4 was a recombinant isolate originating from Kpg3 as a major and B165 as a minor donor. PMID:24426255

  8. Heat shock transcription factors expression during fruit development and under hot air stress in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit.

    PubMed

    Lin, Qiong; Jiang, Qing; Lin, Juanying; Wang, Dengliang; Li, Shaojia; Liu, Chunrong; Sun, Chongde; Chen, Kunsong

    2015-04-01

    Heat shock transcription factors (Hsfs) play a role in plant responses to stress. Citrus is an economically important fruit whose genome has been fully sequenced. So far, no detailed characterization of the Hsf gene family is available for citrus. A genome-wide analysis was carried out in Citrus clementina to identify Hsf genes, named CcHsfs. Eighteen CcHsfs were identified and classified into three main clades (clades A, B and C) according to the structural characteristics and the phylogenetic comparison with Arabidopsis and tomato. MEME motif analysis highlighted the conserved DBD and HR-A/B domains, which were similar to Hsf protein structures in other species. Gene expression analysis in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit identified 14 Hsf genes, named CrHsf, as important candidates for a role in fruit development and ripening, and showed seven genes to be expressed in response to hot air stress. CrHsfB2a and CrHsfB5 were considered to be important regulators of citrate content and showed variation in both developmentally-related and hot air-triggered citrate degradation processes. In summary, the data obtained from this investigation provides the basis for further study to dissect Hsf function during fruit development as well as in response to heat stress and also emphasizes the potential importance of CrHsfs in regulation of citrate metabolism in citrus fruit. PMID:25596345

  9. Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and desiccation in Spinacia oleracea L. cv. Bloomsdale: possible role in stress tolerance.

    PubMed

    Chen, Keting; Fessehaie, Anania; Arora, Rajeev

    2012-02-01

    Osmopriming improves seed germination performance as well as stress tolerance. To understand the biochemistry of osmopriming-induced seed stress tolerance, we investigated dehydrin (DHN) accumulation patterns at protein and transcript level (determined by immunoblotting and qPCR) during priming, and subsequent germination under optimal and stress conditions (i.e. chilling and desiccation) in spinach (Spinacia oleracea L. cv. Bloomsdale) seeds. Our data indicate enhanced germination performance of primed seeds is accompanied by increased accumulation of three dehydrin-like proteins (DLPs): 30, 26, and 19-kD. Moreover, 30, 26 and 19-kD DLPs that first only transiently accumulated during priming re-accumulated in response to stresses, suggesting an evidence for 'cross-tolerance', which is initially induced by priming and later recruited during post-priming germination under stresses. Study with CAP85, a spinach DHN, corroborates above observations at the gene-expression and protein accumulation level. Additionally, our results suggest that during seed germination and seedling establishment, CAP85 expression may be regulated by the interplay of two factors: seedling development and stress responses. In conclusion, our data suggest that 30, 26, and 19-kD dehydrin-like proteins and CAP85 may be used as potential biochemical/molecular markers for priming-induced stress tolerance in 'Bloomsdale' spinach. PMID:22195574

  10. Geographical characterization of greek virgin olive oils (cv. Koroneiki) using 1H and 31P NMR fingerprinting with canonical discriminant analysis and classification binary trees.

    PubMed

    Petrakis, Panos V; Agiomyrgianaki, Alexia; Christophoridou, Stella; Spyros, Apostolos; Dais, Photis

    2008-05-14

    This work deals with the prediction of the geographical origin of monovarietal virgin olive oil (cv. Koroneiki) samples from three regions of southern Greece, namely, Peloponnesus, Crete, and Zakynthos, and collected in five harvesting years (2001-2006). All samples were chemically analyzed by means of 1H and 31P NMR spectroscopy and characterized according to their content in fatty acids, phenolics, diacylglycerols, total free sterols, free acidity, and iodine number. Biostatistical analysis showed that the fruiting pattern of the olive tree complicates the geographical separation of oil samples and the selection of significant chemical compounds. In this way the inclusion of the harvesting year improved the classification of samples, but increased the dimensionality of the data. Discriminant analysis showed that the geographical prediction at the level of three regions is very high (87%) and becomes (74%) when we pass to the thinner level of six sites (Chania, Sitia, and Heraklion in Crete; Lakonia and Messinia in Peloponnesus; Zakynthos). The use of classification and binary trees made possible the construction of a geographical prediction algorithm for unknown samples in a self-improvement fashion, which can be readily extended to other varieties and areas. PMID:18422335

  11. Transmission infrared spectra (2-25 ?m) of carbonaceous chondrites (CI, CM, CV-CK, CR, C2 ungrouped): Mineralogy, water, and asteroidal processes

    NASA Astrophysics Data System (ADS)

    Beck, P.; Garenne, A.; Quirico, E.; Bonal, L.; Montes-Hernandez, G.; Moynier, F.; Schmitt, B.

    2014-02-01

    In this work, infrared transmission spectra (2-25 ?m range, 5000-400 cm-1) of 40 carbonaceous chondrites were analyzed (21 CMs, 5 CVs, 6 CRs, 3 CKs, 3 C2s and 2 CIs). All these meteorite groups are known to have experienced significant aqueous alteration (except the CKs). These IR measurements provide information about the parent body processes experienced, as well as spectra for comparison with observations of Solar System small bodies and possibly with astronomical observations of accretion and debris disks. This study reveals that each meteorite group appears to have specific signatures in the measured IR spectral range. In the case of the CI and CM groups, results show a variability in the shape of the silicate features that can be related to the evolution of the mineralogy with increasing extent of aqueous alteration extent as described by several authors with other techniques. This evolution of the silicate feature can be seen in the variation in the relative intensities of olivine and phyllosilicate IR features. The variability in the silicate features is correlated with the intensity of an -OH related absorption at 3-?m, which can be used for the classification of the meteorites according to the level of hydration. Interestingly, in the case of CM chondrites, for which the mineralogy is expected to be dominated by phyllosilicates (serpentine mostly), the shape of the silicate absorption resembles that of an amorphous silicate, with a broad and symmetric 10-?m band, unlike terrestrial phyllosilicates. The CV and CK groups have IR spectra that are dominated by olivine absorption. From this feature, it is possible to determine average Mg numbers for the olivine. For the CVs, the olivine Mg numbers appear to decrease in the order Kaba-Grosnaja-Vigarano-Mokoia-Allende. This trend is likely related to the long duration of metamorphism experienced by these samples and the chemical re-equilibration between chondritic components. In the case of CK chondrites, the inferred bulk Mg# of olivine is 67 (±1), and no variation is observed between the three studied samples, which is likely related to their high degree of equilibration. The 6 CR chondrites show the most variability in their IR spectra, from CM-like spectra in the case of the CR1 Grosvenor Mountains (GRO) 95577 to CV-like spectra for Roberts Massif (RBT) 04133 and Graves Nunataks (GRA) 06100 (one of them being most probably misclassified). Spectra of the remaining CRs show mixtures of various silicate component. Finally, these spectra can be used for comparison with emission spectra of fine-grained asteroid surfaces and dust-rich cometary tails. In the case of Tempel 1, the only group of CC that matches the observed feature around 10-?m region is the CR group. The spectral comparison shows some striking similarities between CRs and Tempel 1 dust. A genetic link between CR2 and comets is not proven, but mineralogical similarities are suggested from the IR spectra.

  12. Rare Earth Element Transfer from Soil to Navel Orange Pulp (Citrus sinensis Osbeck cv. Newhall) and the Effects on Internal Fruit Quality

    PubMed Central

    Cheng, Jinjin; Ding, Changfeng; Li, Xiaogang; Zhang, Taolin; Wang, Xingxiang

    2015-01-01

    The effects of soil rare earth element (REE) on navel orange quality and safety in rare earth ore areas have gained great attention. This study investigated the transfer characteristics of REE from soil to navel orange pulp (Citrus sinensis Osbeck cv. Newhall) and examined the effects of soil REE on internal fruit quality in Xinfeng County, Jiangxi province, China. Path analysis showed that soil REE, pH, cation exchange capacity (CEC), and Fe oxide (Feox) significantly affected pulp REE concentrations. A Freundlich-type prediction model for pulp REE was established: log[REEpulp] = -1.036 + 0.272 log[REEsoil] - 0.056 pH - 0.360 log[CEC] + 0.370 log[Feox] (n = 114, R2 = 0.60). From the prediction model, it was inferred that even when soil REE and Feox were as high as 1038 mg kg-1 and 96.4 g kg-1, respectively, and pH and CEC were as low as 3.75 and 5.08 cmol kg-1, respectively, pulp REE concentrations were much lower than the food limit standard. Additionally, soil REE levels were significantly correlated with selected fruit quality indicators, including titratable acidity (r = 0.52, P < 0.01), total soluble solids (r = 0.48, P < 0.01) and vitamin C (r = 0.56, P < 0.01). Generally, under routine methods of water and fertilization management, the cultivation of navel oranges in rare earth ore areas of south China with soil REE ranging from 38.6 to 546 mg kg-1 had improved in internal fruit quality. PMID:25806821

  13. Phenylalanine and tyrosine levels are rate-limiting factors in production of health promoting metabolites in Vitis vinifera cv. Gamay Red cell suspension

    PubMed Central

    Manela, Neta; Oliva, Moran; Ovadia, Rinat; Sikron-Persi, Noga; Ayenew, Biruk; Fait, Aaron; Galili, Gad; Perl, Avichai; Weiss, David; Oren-Shamir, Michal

    2015-01-01

    Environmental stresses such as high light intensity and temperature cause induction of the shikimate pathway, aromatic amino acids (AAA) pathways, and of pathways downstream from AAAs. The induction leads to production of specialized metabolites that protect the cells from oxidative damage. The regulation of the diverse AAA derived pathways is still not well understood. To gain insight on that regulation, we increased AAA production in red grape Vitis vinifera cv. Gamay Red cell suspension, without inducing external stress on the cells, and characterized the metabolic effect of this induction. Increased AAA production was achieved by expressing a feedback-insensitive bacterial form of 3-deoxy- D-arabino-heptulosonate 7-phosphate synthase enzyme (AroG*) of the shikimate pathway under a constitutive promoter. The presence of AroG* protein led to elevated levels of primary metabolites in the shikimate and AAA pathways including phenylalanine and tyrosine, and to a dramatic increase in phenylpropanoids. The AroG* transformed lines accumulated up to 20 and 150 fold higher levels of resveratrol and dihydroquercetin, respectively. Quercetin, formed from dihydroquercetin, and resveratrol, are health promoting metabolites that are induced due to environmental stresses. Testing the expression level of key genes along the stilbenoids, benzenoids, and phenylpropanoid pathways showed that transcription was not affected by AroG*. This suggests that concentrations of AAAs, and of phenylalanine in particular, are rate-limiting in production of these metabolites. In contrast, increased phenylalanine production did not lead to elevated concentrations of anthocyanins, even though they are also phenylpropanoid metabolites. This suggests a control mechanism of this pathway that is independent of AAA concentration. Interestingly, total anthocyanin concentrations were slightly lower in AroG* cells, and the relative frequencies of the different anthocyanins changed as well. PMID:26236327

  14. A compound Ca-, Al-rich inclusion from CV3 chondrite Northwest Africa 3118: Implications for understanding processes during CAI formation

    NASA Astrophysics Data System (ADS)

    Ivanova, Marina A.; Lorenz, Cyril A.; Krot, Alexander N.; MacPherson, Glenn J.

    2015-09-01

    A calcium-aluminum-rich inclusion 3N from the Northwest Africa (NWA) 3118 CV3 carbonaceous chondrite is a unique cm-sized compound object, primarily a forsterite-bearing type B (FoB) CAI, that encloses at least 26 smaller CAIs of different types, including compact type A (CTA), B, C, and an ultra-refractory inclusion. Relative to typical type A and B CAIs found elsewhere, the bulk compositions of the types A and B CAIs within 3N more closely match the bulk compositions predicted by equilibrium condensation of a gas of solar composition. Being trapped within the FoB melt may have protected them from melt evaporation that affected most "stand-alone" CAIs. 3N originated either as an aggregate of many smaller (mostly types A, B, C) CAIs plus accreted Fo-bearing material (like an amoeboid olivine aggregate) which experienced partial melting of the whole, or else as a FoB melt droplet that collided with and trapped many smaller solid CAIs. In the former case, 3N recorded the earliest accretion of pebble-sized bodies known. In the latter case, the presence of a large number of individual refractory inclusions within 3N suggests a very high local density of refractory solids in the immediate region of the host CAI during the brief time while it was melted. Collisions would have occurred on time scales of hours at most, assuming a melt solidification interval for the host CAI of 300-400 °C (maximum) and a cooling rate of ~10 °C/h.

  15. Profiling monoterpenol glycoconjugation in Vitis vinifera L. cv. Muscat of Alexandria using a novel putative compound database approach, high resolution mass spectrometry and collision induced dissociation fragmentation analysis.

    PubMed

    Hjelmeland, Anna K; Zweigenbaum, Jerry; Ebeler, Susan E

    2015-08-01

    In this work we present a novel approach for the identification of plant metabolites using ultrahigh performance liquid chromatography coupled to accurate mass time-of-flight mass spectrometry. The workflow involves developing an in-house compound database consisting of exact masses of previously identified as well as putative compounds. The database is used to screen accurate mass spectrometry (MS) data to identify possible compound matches. Subsequent tandem MS data is acquired for possible matches and used for structural elucidation. The methodology is applied to profile monoterpene glycosides in Vitis vinifera cv. Muscat of Alexandria grape berries over three developmental stages. Monoterpenes are a subclass of terpenes, the largest class of plant secondary metabolites, and are found in two major forms in the plant, "bound" to one or more sugar moieties or "free" of said sugar moieties. In the free form, monoterpenes are noted for their fragrance and play important roles in plant defense and as attractants for pollinators. However, glycoconjugation renders these compounds odorless, and it is this form that the plant uses for monoterpene storage. In order to gain insight into monoterpene biochemistry and their fate in the plant an analysis of intact glycosides is essential. Eighteen monoterpene glycosides were identified including a monoterpene trisaccharide glycoside, which is tentatively identified here for this first time in any plant. Additionally, while previous studies have identified monoterpene malonylated glucosides in other grapevine tissue, we tentatively identify them for the first time in grape berries. This analytical approach can be readily applied to other plants and the workflow approach can also be used for other classes of compounds. This approach, in general, provides researchers with data to support the identification of putative compounds, which is especially useful when no standard is available. PMID:26320795

  16. Improved Maturity and Ripeness Classifications of Magnifera Indica cv. Harumanis Mangoes through Sensor Fusion of an Electronic Nose and Acoustic Sensor

    PubMed Central

    Zakaria, Ammar; Shakaff, Ali Yeon Md; Masnan, Maz Jamilah; Saad, Fathinul Syahir Ahmad; Adom, Abdul Hamid; Ahmad, Mohd Noor; Jaafar, Mahmad Nor; Abdullah, Abu Hassan; Kamarudin, Latifah Munirah

    2012-01-01

    In recent years, there have been a number of reported studies on the use of non-destructive techniques to evaluate and determine mango maturity and ripeness levels. However, most of these reported works were conducted using single-modality sensing systems, either using an electronic nose, acoustics or other non-destructive measurements. This paper presents the work on the classification of mangoes (Magnifera Indica cv. Harumanis) maturity and ripeness levels using fusion of the data of an electronic nose and an acoustic sensor. Three groups of samples each from two different harvesting times (week 7 and week 8) were evaluated by the e-nose and then followed by the acoustic sensor. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were able to discriminate the mango harvested at week 7 and week 8 based solely on the aroma and volatile gases released from the mangoes. However, when six different groups of different maturity and ripeness levels were combined in one classification analysis, both PCA and LDA were unable to discriminate the age difference of the Harumanis mangoes. Instead of six different groups, only four were observed using the LDA, while PCA showed only two distinct groups. By applying a low level data fusion technique on the e-nose and acoustic data, the classification for maturity and ripeness levels using LDA was improved. However, no significant improvement was observed using PCA with data fusion technique. Further work using a hybrid LDA-Competitive Learning Neural Network was performed to validate the fusion technique and classify the samples. It was found that the LDA-CLNN was also improved significantly when data fusion was applied. PMID:22778629

  17. Improved maturity and ripeness classifications of Magnifera Indica cv. Harumanis mangoes through sensor fusion of an electronic nose and acoustic sensor.

    PubMed

    Zakaria, Ammar; Shakaff, Ali Yeon Md; Masnan, Maz Jamilah; Saad, Fathinul Syahir Ahmad; Adom, Abdul Hamid; Ahmad, Mohd Noor; Jaafar, Mahmad Nor; Abdullah, Abu Hassan; Kamarudin, Latifah Munirah

    2012-01-01

    In recent years, there have been a number of reported studies on the use of non-destructive techniques to evaluate and determine mango maturity and ripeness levels. However, most of these reported works were conducted using single-modality sensing systems, either using an electronic nose, acoustics or other non-destructive measurements. This paper presents the work on the classification of mangoes (Magnifera Indica cv. Harumanis) maturity and ripeness levels using fusion of the data of an electronic nose and an acoustic sensor. Three groups of samples each from two different harvesting times (week 7 and week 8) were evaluated by the e-nose and then followed by the acoustic sensor. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were able to discriminate the mango harvested at week 7 and week 8 based solely on the aroma and volatile gases released from the mangoes. However, when six different groups of different maturity and ripeness levels were combined in one classification analysis, both PCA and LDA were unable to discriminate the age difference of the Harumanis mangoes. Instead of six different groups, only four were observed using the LDA, while PCA showed only two distinct groups. By applying a low level data fusion technique on the e-nose and acoustic data, the classification for maturity and ripeness levels using LDA was improved. However, no significant improvement was observed using PCA with data fusion technique. Further work using a hybrid LDA-Competitive Learning Neural Network was performed to validate the fusion technique and classify the samples. It was found that the LDA-CLNN was also improved significantly when data fusion was applied. PMID:22778629

  18. Effect of Three Training Systems on Grapes in a Wet Region of China: Yield, Incidence of Disease and Anthocyanin Compositions of Vitis vinifera cv. Cabernet Sauvignon.

    PubMed

    Liu, Mei-Ying; Chi, Ming; Tang, Yong-Hong; Song, Chang-Zheng; Xi, Zhu-Mei; Zhang, Zhen-Wen

    2015-01-01

    Grapevine training systems determine the suitability for grape varieties in a specific growing region. We evaluated the influence of three training systems, Single Guyot (SG), Spur-pruned Vertical Shoot-Positioned (VSP), and Four-Arm Kniffin (4AK), on the performance of grapes and vines of Vitis vinifera L. cv. Cabernet Sauvignon in the 2012 and 2013 growing seasons in a wet region of central China. 4AK was the most productive system in comparison to SG and VSP. SG and VSP had lower disease infections of leaves and berries, especially in the mid- and final stage of berry ripening. Three training systems had no impact on berry maturity. PLS-DA (Partial Least Squares-Discriminant) analysis showed that the relatively dry vintage could well discriminate three training systems, but the wet vintage was not. A wet vintage of 2013 had more accumulation of 3'5'-substituted and acylated anthocyanins, including malvidin-3-O-(6-O-acetyl)-glucoside, malvidin-3-O-glucoside, and petunidin-3-O-(cis-6-O-coumaryl)-glucoside, etc. With regard to the effect of training systems, 4AK grapes had the lowest concentrations of total anthocyanins and individual anthocyanins, SG and VSP differed according to the different vintages, and showed highest concentration of total individual anthocyanins in 2012 and 2013, respectively. Generally, VSP benefited the most, contributing to significantly highest levels of total individual anthocyanins, and major anthocyanin, including malvidin-3-O-glucoside and malvidin-3-O-(6-O-acetyl)-glucoside, and the grapes obtained from VSP presented significantly highest proportion of 3'5'-substituted anthocyanins. With regard to the ratios of 3'5'/3'-substituted, methoxylated/non-methoxylated and acylated/non-acylated anthocyanins, the significantly higher levels were also shown in VSP system. In summary, VSP was the best training system for Cabernet Sauvignon to accumulate relatively stable individual anthocyanins in this wet region of China and potentially in other rainy regions. PMID:26492226

  19. A role for PacMYBA in ABA-regulated anthocyanin biosynthesis in red-colored sweet cherry cv. Hong Deng (Prunus avium L.).

    PubMed

    Shen, Xinjie; Zhao, Kai; Liu, Linlin; Zhang, Kaichun; Yuan, Huazhao; Liao, Xiong; Wang, Qi; Guo, Xinwei; Li, Fang; Li, Tianhong

    2014-05-01

    The MYB transcription factors and plant hormone ABA have been suggested to play a role in fruit anthocyanin biosynthesis, but supporting genetic evidence has been lacking in sweet cherry. The present study describes the first functional characterization of an R2R3-MYB transcription factor, PacMYBA, from red-colored sweet cherry cv. Hong Deng (Prunus avium L.). Transient promoter assays demonstrated that PacMYBA physically interacted with several anthocyanin-related basic helix-loop-helix (bHLH) transcription factors to activate the promoters of PacDFR, PacANS and PacUFGT, which are thought to be involved in anthocyanin biosynthesis. Furthermore, the immature seeds of transgenic Arabidopsis plants overexpressing PacMYBA exhibited ectopic pigmentation. Silencing of PacMYBA, using a Tobacco rattle virus (TRV)-induced gene silencing technique, resulted in sweet cherry fruit that lacked red pigment. ABA treatment significantly induced anthocyanin accumulation, while treatment with the ABA biosynthesis inhibitor nordihydroguaiaretic acid (NDGA) blocked anthocyanin production. PacMYBA expression peaked after 2 h of pre-incubation in ABA and was 15.2-fold higher than that of sweet cherries treated with NDGA. The colorless phenotype was also observed in the fruits silenced in PacNCED1, which encodes a key enzyme in the ABA biosynthesis pathway. The endogenous ABA content as well as the transcript levels of six structural genes and PacMYBA in PacNCED1-RNAi (RNA interference) fruit were significantly lower than in the TRV vector control fruit. These results suggest that PacMYBA plays an important role in ABA-regulated anthocyanin biosynthesis and ABA is a signal molecule that promotes red-colored sweet cherry fruit accumulating anthocyanin. PMID:24443499

  20. Catabolism of (+/-)-abscisic acid by excised leaves of Hordeum vulgare L. cv Dyan and its modification by chemical and environmental factors

    SciTech Connect

    Cowan, A.K.; Railton, I.D.

    1987-05-01

    Excised light-grown leaves and etiolated leaves of Hordeum vulgare L. cv Dyan catabolized applied (+/-)-(2-/sup 14/C)abscisic acid ((+/-)-(2-/sup 14/C)ABA) to phaseic acid (PA), dihydrophaseic acid (DPA), and 2'-hydroxymethyl ABA (2'-HMABA). Identification of these catabolites was made by microchemical methods and by combined capillary gas chromatography-mass spectrometry (GC-MS) following high dose feeds of nonlabeled substrate to leaves. Circular dichroism analysis revealed that 2'-HMABA was derived from the (-) enantiomer of ABA. Refeeding studies were used to confirm the catabolic route. The methyl ester of (+/-)-(2/sup 14/C)-ABA was hydrolyzed efficiently by light-grown leaves of H. vulgare. Leaf age played a significant role in (+/-)-ABA catabolism, with younger leaves being less able than their older counterparts to catabolize this compound. The catabolism of (+/-)-ABA was inhibited markedly in water-stressed Hordeum leaves which was characterized by a decreased incorporation of label into 2'-HMABA, DPA, and conjugates. The specific, mixed function oxidase inhibitor, ancymidol, did not inhibit, dramatically (+/-)-ABA catabolism in light-grown leaves of Hordeum whereas the 80s ribosome, translational inhibitor, cycloheximide, inhibited this process markedly. The 70s ribosome translational inhibitors, lincomycin and chloramphenicol, were less effective than cycloheximide in inhibiting (+/-)-ABA catabolism, implying that cytoplasmic protein synthesis is necessary for the catabolism of (+/-)-ABA in Hordeum leaves whereas chloroplast protein synthesis plays only a minor role. This further suggests that the enzymes involved in (+/-)-ABA catabolism in this plant are cytoplasmically synthesized and are turned-over rapidly, although the enzyme responsible for glycosylating (+/-)-ABA itself appeared to be stable.

  1. Significant changes in cell and chloroplast development in young wheat leaves (Triticum aestivum cv Hereward) grown in elevated CO{sub 2}

    SciTech Connect

    Robertson, E.J.; Leech, R.M.

    1995-01-01

    Cell and chloroplast development were characterized in young Triticum aestivum cv Hereward leaves grown at ambient (350 {mu}L L{sup {minus}1}) or at elevated (650 {mu}L L{sup {minus}1}) CO{sub 2}. In elevated CO{sub 2}, cell and chloroplast expansion was accelerated by 10 and 25%, respectively, in the first leaf of 7-d-old wheat plants without disruption to the leaf developmental pattern. Elevated CO{sub 2} did not affect the number of chloroplasts in relation to mesophyll cell size or the linear relationship between chloroplast number or size and mesophyll cell size. No major changes in leaf anatomy or in chloroplast ultrastructure were detected as a result of growth in elevated CO{sub 2}, but there was a marked reduction in starch accumulation. In leaf sections fluorescently tagged antisera were used to visualize and quantitate the amount of cytochrome f, the {alpha}- and {beta}-subunits of the coupling factor 1 in ATP synthase, D1 protein of the photosystem II reaction center, the 33-kD protein of the extrinsic oxygen-evolving complex, subunit II of photosystem I, and ribulose-1,5-biphosphate carboxylase/oxygenase. A significant finding was that in 10 to 20% of the mesophyll cells grown in elevated CO{sub 2} the 33-kD protein of the extrinsic oxygen-evolving complex of photosystem II and cytochrome f were deficient by 75%, but the other proteins accumulated normally. 29 refs., 6 figs., 2 tabs.

  2. Salicylic Acid Alleviates the Adverse Effects of Salt Stress in Torreya grandis cv. Merrillii Seedlings by Activating Photosynthesis and Enhancing Antioxidant Systems

    PubMed Central

    Du, Xuhua; Tang, Hui; Shen, Chaohua; Wu, Jiasheng

    2014-01-01

    Background Salt stress is a major factor limiting plant growth and productivity. Salicylic acid (SA) has been shown to ameliorate the adverse effects of environmental stress on plants. To investigate the protective role of SA in ameliorating salt stress on Torreya grandis (T. grandis) trees, a pot experiment was conducted to analyze the biomass, relative water content (RWC), chlorophyll content, net photosynthesis (Pn), gas exchange parameters, relative leakage conductivity (REC), malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD) and peroxidase (POD) of T. grandis under 0.2% and 0.4% NaCl conditions with and without SA. Methodology/Principal Findings The exposure of T. grandis seedlings to salt conditions resulted in reduced growth rates, which were associated with decreases in RWC and Pn and increases in REC and MDA content. The foliar application of SA effectively increased the chlorophyll (chl (a+b)) content, RWC, net CO2 assimilation rates (Pn), and proline content, enhanced the activities of SOD, CAT and POD, and minimized the increases in the REC and MDA content. These changes increased the capacity of T. grandis in acclimating to salt stress and thus increased the shoot and root dry matter. However, when the plants were under 0% and 0.2% NaCl stress, the dry mass of the shoots and roots did not differ significantly between SA-treated plants and control plants. Conclusions SA induced the salt tolerance and increased the biomass of T. grandis cv. by enhancing the chlorophyll content and activity of antioxidative enzymes, activating the photosynthetic process, and alleviating membrane injury. A better understanding about the effect of salt stress in T. grandis is vital, in order gain knowledge over expanding the plantations to various regions and also for the recovery of T. grandis species in the future. PMID:25302987

  3. The influence of crop management on banana weevil, Cosmopolites sordidus (Coleoptera: Curculionidae) populations and yield of highland cooking banana (cv. Atwalira) in Uganda.

    PubMed

    Rukazambuga, N D T M; Gold, C S; Gowen, S R; Ragama, P

    2002-10-01

    A field study was undertaken in Uganda using highland cooking banana (cv. Atwalira) to test the hypothesis that bananas grown under stressed conditions are more susceptible to attack by Cosmopolites sordidus (Germar). Four banana treatments were employed to create different levels of host-plant vitality: (1) high stress: intercrop with finger millet; (2) moderate stress: monoculture without soil amendments; (3) low stress: monoculture with manure; (4) high vigour: monoculture with continuous mulch and manure. Adult C. sordidus were released at the base of banana mats 11 months after planting and populations were monitored for three years using mark and recapture methods. Cosmopolites sordidus density was greatest in the mulched plots which may have reflected increased longevity and/or longer tenure time in moist soils. Lowest C. sordidus numbers were found in intercropped banana. Damage, estimated as percentage corm tissue consumed by larvae, was similar among treatments. However, the total amount of tissue consumed was greater in mulched banana than in other systems. Plants supporting the heaviest levels of C. sordidus damage displayed bunch size reductions of 40-55%. Banana yield losses ranged from 14-20% per plot with similar levels in the intercropped and mulched systems. Yield reductions, reported as t ha-1, were twice as high in the mulched system as in the intercrop. The results from this study indicate that C. sordidus problems are not confined to stressed banana systems or those with low levels of management, but that the weevil can also attain pest status in well-managed and productive banana stands. PMID:12241566

  4. Does nitrogen supply affect the response of wheat (Triticum aestivum cv. Hanno) to the combination of elevated CO(2) and O(3)?

    PubMed

    Cardoso-Vilhena, J; Barnes, J

    2001-09-01

    Spring wheat (Triticum aestivum cv. Hanno) was grown at ambient (350 micromol mol(-1)) or elevated CO(2) (700 micromol mol(-1)) in charcoal/Purafil-filtered air (CFA <5 nmol mol(-1)) or ozone (CFA +75 nmol mol(-1) 7 h d(-1)) at three levels of N supply (1.5, 4 and 14 mM NO(-3)), to test the hypothesis that the combined impacts of elevated CO(2) and O(3) on plant growth and photosynthetic capacity are affected by nitrogen availability. Shifts in foliar N content reflected the level of N supplied, and the growth stimulation induced by elevated CO(2) was dependent on the level of N supply. At 60 d after transfer (DAT), elevated CO(2) was found to increase total biomass by 44%, 29%, 12% in plants supplied with 14, 4 and 1.5 mM NO(-3), respectively, and there was no evidence of photosynthetic acclimation to elevated CO(2) across N treatments; the maximum in vivo rate of Rubisco carboxylation (V(cmax)) was similar in plants raised at elevated and ambient CO(2). At 60 DAT, ozone exposure was found to suppress plant relative growth rate (RGR) and net photosynthesis (A) in plants supplied with 14 and 4 mM NO(-3). However, O(3) had no effect on the RGR of plants supplied with 1.5 mM NO(-3) and this effect was accompanied by a reduced impact of the pollutant on A. Elevated CO(2) counteracted the detrimental effects of O(3) (i.e. the same ozone concentration that depressed RGR and A at ambient CO(2) resulted in no significant effects when plants were raised at elevated CO(2)) at all levels of N supply and the effect was associated with a decline in O(3) uptake at the leaf level. PMID:11520879

  5. TRANSIENT EXTREMELY SOFT X-RAY EMISSION FROM THE UNUSUALLY BRIGHT CATACLYSMIC VARIABLE IN THE GLOBULAR CLUSTER M3: A NEW CV X-RAY LUMINOSITY RECORD?

    SciTech Connect

    Stacey, W. S.; Heinke, C. O.; Elsner, R. F.; Weisskopf, M. C.; Edmonds, P. D.; Grindlay, J. E.

    2011-05-01

    We observed the accreting white dwarf (WD) 1E1339.8+2837 (1E1339) in the globular cluster M3 in 2003 November, 2004 May, and 2005 January, using the Chandra ACIS-S detector. The source was observed in 1992 to possess traits of a supersoft X-ray source (SSS), with a 0.1-2.4 keV luminosity as large as 2 x 10{sup 35} erg s{sup -1}, after which time the source's luminosity fell by roughly two orders of magnitude, adopting a hard X-ray spectrum more typical of cataclysmic variables (CVs). Our observations confirm 1E1339's hard CV-like spectrum, with photon index {Gamma} = 1.3 {+-} 0.2. We found 1E1339 to be highly variable, with a 0.5-10 keV luminosity ranging from (1.4 {+-} 0.3) x 10{sup 34} erg s{sup -1} to 8.5{sup +4.9}{sub -4.6} x 10{sup 32} erg s{sup -1}, with 1E1339's maximum luminosity being perhaps the highest yet recorded for hard X-ray emission from a WD. In 2005 January, 1E1339 displayed substantial low-energy emission below {approx}0.3 keV. Although current Chandra responses cannot properly model this emission, its bolometric luminosity appears comparable to or greater than that of the hard spectral component. This raises the possibility that the supersoft X-ray emission seen from 1E1339 in 1992 may have shifted to the far-UV.

  6. Curdlan ?-1,3-Glucooligosaccharides Induce the Defense Responses against Phytophthora infestans Infection of Potato (Solanum tuberosum L. cv. McCain G1) Leaf Cells

    PubMed Central

    Li, Jing; Zhu, Li; Lu, Guangxing; Zhan, Xiao-Bei; Lin, Chi-Chung; Zheng, Zhi-Yong

    2014-01-01

    Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H2O2 and salicylic acid and the activities of phenylalanine amino-lyase, ?-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually. PMID:24816730

  7. Mahtab Jafari CV Curriculum Vitae

    E-print Network

    Chen, Zhongping

    ://files.onset.freedom.com/ocregister/Focus/20130626_Focus.pdf Yahoo! Voices. July 5, 2013. Rhodila rosea Furthers Lifespan of Fruit Flies by 24% http://voices.yahoo.com/rhodiola-rosea-furthers-lifespan-fruit-flies-by-12208012.html?cat=5 MSN News. July 2013. Scientists discover plants make flies live longer, Fad to follow http://now.msn.com/rhodiola-increases-lifespan-of-fruit-flies

  8. Owen CV -1 Curriculum Vitae

    E-print Network

    Horswill, Ian

    -direct Capital Punishment Center. Teach classes on Capital Punishment, Advanced Topics in Capital Punishment, and The Right to Counsel and the Death Penalty. Co-teach Capital Punishment Clinic class and supervise clinical in the undergraduate Plan II Honors Program on the cultural life of capital punishment. Student evaluations: "one

  9. CV S. Murawski CURRICULUM VITAE

    E-print Network

    Meyers, Steven D.

    fishermen's behavior and choice, and investing the short- and long-term effects of the Deepwater Horizon oil of the Gulf of Mexico ever undertaken, and continue to analyze the impacts of Deepwater Horizon on Gulf fish

  10. [Abbreviated CV] David L. Seim

    E-print Network

    Wu, Mingshen

    Ph.D. Program in the History of Technology & Science, History Dept., Iowa State University, 2007 & Science M.A., History, Iowa State University, 2001 M.A., Economics, Colorado State University, 1996 Fields major in Psychology TEACHING Primary Fields History of Science & Technology History of Social Science

  11. CV LIVIO RUGGIERO WORK EXPERIENCE

    E-print Network

    OfficeTM tools (WordTM, ExcelTM and PowerPointTM); Graphic design applications (Adobe Illustrator Filippacio-Il Casone- North Lazio" PERSONAL SKILLS Mother tongue Italian Other languages English - Spanish Communication skills Team spirit and capability in problem solving; I have always worked in a team

  12. CV Sergienko 1 Curriculum Vitae

    E-print Network

    Rodgers, Keith

    . Dissertation: Surface melting on ice shelves and icebergs. Advisor: Douglas R. MacAyeal 1999 Candidate sponsored, David E. Archer, P.I.), Collaborative Research on Earth's Largest Icebergs (NSF sponsored experience Austral summers, three seasons, 2004-2006: Icebergs of the Ross Sea, the Ross Ice Shelf and Mc

  13. Nerium oleander cv. Album (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    square foot. Other Considerations: Do not allow to dry out. VARIETIES : Heavenly Blue. Album. COST OF PRODIJCTION: Storage cost: 6 weeks at GC = 364. 36 .+ 16 = 2.254 per pot. Forcing cost: 3 weeks at 54 = 1%. 154 + 6 = 2.54 per plant. Total... markets For L' a TEXAS AGRICULTURAL EXPERIMENT STATION L .. - " : I: Summary There is no established custom in the United States of using plants and flowers in the average home. Such customs take a long period of development. The florist industry...

  14. Trubanova CV 1 Andrea Trubanova

    E-print Network

    the lack of relationship between vaccines and autism diagnosis within a longitudinal sample Collected eye.D. Clinical Research Coordinator Marcus Autism Center, Atlanta, GA Managed all regulatory processes related. Cohen Fellow in Developmental Social Neuroscience Marcus Autism Center, Children's Healthcare of Atlanta

  15. FERNANDO GUIRAO CV Contact Information

    E-print Network

    . Hard copy and e-Book editions. 2. (with Frances M.B. Lynch and Sigfrido Ramírez) (eds.), Alan S and Canada). E-book version ISBN 9780333710784. Publications, chapters in books 1. "An Independent Catalonia

  16. Rosa cv. Fragrant Cloud (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    This dissertation reports on three different yet related topics in light scattering computation, radiative transfer simulation, and remote sensing implementation, regarding the cloud properties and the retrieval of cloud properties from satellite...

  17. Gilbert CV 1 BENJAMIN GILBERT

    E-print Network

    Gilbert, Benjamin

    : the positive correlation between native and exotic plant diversity. Ecology 86: 1848-1855. Karst*, J., B equally. Weber, A., J. Karst, B. Gilbert and J.P. Kimmins. 2005. Thuja plicata exclusion in ectomycorrhiza

  18. CV Velemir Ninkovic Nationality: Swedish

    E-print Network

    , Sweden BSc Agronomy, July 1990, Agricultural Engineer for Farming, University of Sarajevo, Bosnia University of Agricultural Sciences, SLU, Alnarp, Sweden, Mars 22, 2013, Teaching - Ecological Concept

  19. The Rhetoric of the CV

    ERIC Educational Resources Information Center

    Eyler, Joshua R.

    2012-01-01

    When a person sends in his or her job-application materials, he or she is not just assembling separate documents to fulfill the requirements of an ad. Those documents are part of a larger rhetorical whole, and together they form an argument for the viability of his or her candidacy for a particular job. Documents should be crafted meticulously,…

  20. Solanum quitoense cv. Lulo (Cultivated) 

    E-print Network

    Hugh D. Wilson

    2011-08-10

    With proper management, alfalfa will produce forage with the highest protein and total digestible nutrient of any hay crop. To aid in alfalfa production, this publication provides information on pre-plant factors, planting ...

  1. Georgi Kapitanov CV Georgi Kapitanov

    E-print Network

    , A Mathematical Model of Cancer Stem Cell Lineage Population Dynamics with Mutation Accumulation and Telomere of Tennessee, TN, USA, 2013 A Mathematical Model of Cancer Stem Cell Lineage Population Dynamics with Mu, Sewanee - the University of the South, Sewanee, TN, USA, 2012 A Mathematical Model of Cancer Stem Cell

  2. Dan Fleetwood, CV Faculty Office

    E-print Network

    Research Interests Effects of ionizing radiation on microelectronic devices & materials - 31 yrs. Novel yrs. Defects, reliability, radiation response of SiC, GaN, and other compound semiconductor devices, Albuquerque, NM, Distinguished/Senior Member of Technical Staff, Radiation Technology & Assurance Dept. (SMTS

  3. Bracken CV -1 Curriculum Vitae

    E-print Network

    Chen, Zhongping

    .E.S. and S.L. Williams. 2013. Realistic changes in seaweed biodiversity affect multiple ecosystem functions.E.S. Bracken, M. McConville* , K. Rodrigue* , and C.S. Thornber. 2013. Invasion of the red seaweed on intertidal seaweed diversity

  4. Pyrus communis cv. Bartlett (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    by reduced and oxidized glutathione, and cell cycle processes, proliferation and apoptosis, in the rat colon. Total SOD activity was higher (p < 0.04) in rats consuming black sorghum when compared to all other diets. A similar, but not significant, trend...

  5. Growth, yield and quality attributes of a tropical potato variety (Solanum tuberosum L. cv Kufri chandramukhi) under ambient and elevated carbon dioxide and ozone and their interactions.

    PubMed

    Kumari, Sumita; Agrawal, Madhoolika

    2014-03-01

    The present study was designed to study the growth and yield responses of a tropical potato variety (Solanum tuberosum L. cv. Kufri chandramukhi) to different levels of carbon dioxide (382 and 570ppm) and ozone (50 and 70ppb) in combinations using open top chambers (OTCs). Plants were exposed to three ozone levels in combination with ambient CO2 and two ozone levels at elevated CO2. Significant increments in leaf area and total biomass were observed under elevated CO2 in combination with ambient O3 (ECO2+AO3) and elevated O3 (ECO2+EO3), compared to the plants grown under ambient concentrations (ACO2+AO3). Yield measured as fresh weight of potato also increased significantly under ECO2+AO3 and ECO2+EO3. Yield, however, reduced under ambient (ACO2+AO3) and elevated ozone (ACO2+EO3) compared to ACO2 (filtered chamber). Number, fresh and dry weights of tubers of size 35-50mm and>50mm used for direct consumption and industrial purposes, respectively increased maximally under ECO2+AO3. Ambient as well as elevated levels of O3 negatively affected the growth parameters and yield mainly due to reductions in number and weight of tubers of sizes >35mm. The quality of potato tubers was also modified under different treatments. Starch content increased and K, Zn and Fe concentrations decreased under ECO2+AO3 and ECO2+EO3 compared to ACO2+AO3. Starch content reduced under ACO2+AO3 and ACO2+EO3 treatments compared to ACO2. These results clearly suggest that elevated CO2 has provided complete protection to ambient O3 as the potato yield was higher under ECO2+AO3 compared to ACO2. However, ambient CO2 is not enough to protect the plants under ambient O3 levels. Elevated CO2 also provided protection against elevated O3 by improving the yield. Quality of tubers is modified by both CO2 and O3, which have serious implications on human health at present and in future. PMID:24507140

  6. Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86

    PubMed Central

    2013-01-01

    Background Flour quality is largely determined by the gluten proteins, a complex mixture of proteins consisting of high molecular weight-glutenin subunits (HMW-GS), low molecular weight-glutenin subunits (LMW-GS), and ?-, ?-, and ?-gliadins. Detailed proteomic analyses of the effects of fertilizer and high temperature on individual gliadin and glutenin protein levels are needed to determine how these environmental factors influence flour quality. Results Wheat plants (Triticum aestivum L. cv. Butte 86) were grown in greenhouses under moderate and high temperature regimens with and without post-anthesis fertilizer. Quantitative two-dimensional gel electrophoresis was used to construct accumulation profiles in developing endosperm for the entire complement of gluten proteins identified previously by tandem mass spectrometry. Amounts of individual gliadins and glutenins were also determined in flour produced under each of the regimens. Under all environmental regimens, most HMW-GS, LMW-GS, ?- and ?-gliadins accumulated rapidly during early stages of grain development and leveled off during middle stages of development. A subset of LMW-GS showed a second distinct profile, accumulating throughout development, while ?-gliadins showed a variety of accumulation profiles. In flour, fourteen distinct gluten proteins responded similarly to fertilizer, high temperature, and high temperature plus fertilizer. The majority of HMW-GS and ?-gliadins and some ?-gliadins increased while two LMW-GS and a minor ?-gliadin decreased. Fertilizer did not influence gluten protein accumulation under high temperature conditions. Additionally, the effects of fertilizer and high temperature were not additive; very few changes were observed when plants that received fertilizer were subjected to high temperature. Conclusions Although post-anthesis temperature and fertilizer have very different effects on grain development and yield, the two treatments elicit surprisingly similar effects on the accumulation of gluten proteins. The similarity of the responses to the different treatments is likely due to source-sink activities of nitrogen reserves in the wheat plant. Because each protein that showed a response in this study is linked to a gene sequence, the work sets the stage for transgenic studies that will better elucidate the roles of specific proteins in flour quality and in the response to the environment. PMID:23432757

  7. The formation of Ca-, Fe-rich silicates in reduced and oxidized CV chondrites: The roles of impact-modified porosity and permeability, and heterogeneous distribution of water ices

    NASA Astrophysics Data System (ADS)

    MacPherson, Glenn J.; Krot, Alexander N.

    2014-07-01

    CV (Vigarano type) carbonaceous chondrites, comprising Allende-like (CVoxA) and Bali-like (CVoxB) oxidized and reduced (CVred) subgroups, experienced differing degrees of fluid-assisted thermal and shock metamorphism. The abundance and speciation of secondary minerals produced during asteroidal alteration differ among the subgroups: (1) ferroan olivine and diopside-hedenbergite solid solution pyroxenes are common in all CVs; (2) nepheline and sodalite are abundant in CVoxA, rare in CVred, and absent in CVoxB; (3) phyllosilicates and nearly pure fayalite are common in CVoxB, rare in CVred, and virtually absent in CVoxA; (4) andradite, magnetite, and Fe-Ni-sulfides are common in oxidized CVs, but rare in reduced CVs; the latter contain kirschsteinite instead. Thus, a previously unrecognized correlation exists between meteorite bulk permeabilities and porosities with the speciation of the Ca-, Fe-rich silicates (pyroxenes, andradite, kirschsteinite) among the CVox and CVred meteorites. The extent of secondary mineralization was controlled by the distribution of water ices, permeability, and porosity, which in turn were controlled by impacts on the asteroidal parent body. More intense shock metamorphism in the region where the reduced CVs originated decreased their porosity and permeability while simultaneously expelling intergranular ices and fluids. The mineralogy, petrography, and bulk chemical compositions of both the reduced and oxidized CV chondrites indicate that mobile elements were redistributed between Ca,Al-rich inclusions, dark inclusions, chondrules, and matrices only locally; there is no evidence for large-scale (>several cm) fluid transport. Published 53Mn-53Cr ages of secondary fayalite in CV, CO, and unequilibrated ordinary chondrites, and carbonates in CI, CM, and CR chondrites are consistent with aqueous alteration initiated by heating of water ice-bearing asteroids by decay of 26Al, not shock metamorphism.

  8. Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. 'Tommy Atkins') peels, pulp and kernels by high-performance liquid chromatography/electrospray ionization mass spectrometry.

    PubMed

    Berardini, Nicolai; Carle, Reinhold; Schieber, Andreas

    2004-01-01

    Polyphenolics were extracted from peels, pulp and kernels of mango fruits (Mangifera indica L. cv. 'Tommy Atkins') and characterized by high-performance liquid chromatography/electrospray ionization mass spectrometry. In the peel 18 gallotannins and five benzophenone derivatives were detected which were tentatively identified as galloylated maclurin and iriflophenone glucosides. Twenty-one and eight gallotannins were found in the kernels and pulp, respectively, whereas no evidence for the presence of benzophenone derivatives was obtained. Gallotannins quantified by the rhodanine assay amounted to 1.4 mg/g dm in the peels (expressed as gallic acid), while only small amounts (0.2 mg/g dm) were found in the pulp. In contrast, mango kernels contained 15.5 mg/g dm and thus proved to be a rich source of gallotannins. PMID:15384138

  9. High resolution mass spectrometry imaging reveals the occurrence of phenylphenalenone-type compounds in red paracytic stomata and red epidermis tissue of Musa acuminata ssp. zebrina cv. 'Rowe Red'.

    PubMed

    Hölscher, Dirk; Fuchser, Jens; Knop, Katrin; Menezes, Riya C; Buerkert, Andreas; Svatoš, Aleš; Schubert, Ulrich S; Schneider, Bernd

    2015-08-01

    The banana epidermis and in particular their stomata are conducive sites for the penetration of pathogenic fungi which can severely limit global banana production. The red pseudostem of the ornamental banana Musa acuminata ssp. zebrina cv. 'Rowe Red' was used to study the chemical constituents of the epidermal cell layer using matrix-free laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometric imaging (LDI-FT-ICR-MSI). The high resolution of this technique allowed phenylphenalenone-type compounds to be located in single plant cells. Some of these secondary metabolites were identified as constitutive compounds and found in specialized epidermal cells in banana pseudostem tissue. Especially the red paracytic stomata revealed higher signal intensities of certain phenylphenalenones than normal epidermis cells. The ease of detection of polycyclic aromatic compounds on the cellular level is discussed with regard to future investigations of plant-pathogen interactions. PMID:26004822

  10. [Studies on the performance of the dental air turbine handpieces. (Part 5) Rotational performance of various kinds of dental air turbine handpieces (N = N0--CvFv gamma) (author's transl)].

    PubMed

    Miyairi, H; Nagai, M; Fukuda, H; Muramatsu, A

    1981-10-01

    Making research on the rotational performance of the dental air turbine handpieces widely used for dental cutting instruments, we investigated with the relation between rotational speed and load on many kinds of dental handpieces used for clinical divisions and with the applications of the theoretical equations to have be shown in the previous report. And then we have the investigation on these properties. Dental handpieces to measured with these properties were classified the four types, standard types, torque type, miniature type and air bearing type. And we applied the experimental equations to these experimental values and determined the rotational coefficients, gamma and Cv. The rotational speed N and normal force Fv, which occurred the maximum work of air turbine handpieces, were calculated with our analysis and then these values of N and Fv were compared with the experimental results. These results, that is, experimental and calculated values have a good agreement and the development of the theoretical approach is useful for the estimation of the rotational performance. And we examined the possibility of rotational performance's estimation with the process of simple experiment on these air turbine handpieces. PMID:7037999

  11. The effects on photosynthetic CO{sub 2} assimilation to long-term elevation of atmospheric CO{sub 2} concentration: An assessment of the response of Trifolium Repens L. cv. Blanca grown at F.A.C.E.

    SciTech Connect

    Lewis, C.E.

    1994-11-01

    Understanding how photosynthetic capacity acclimates to elevated CO{sub 2} concentrations is vital in predicting the response of important grassland species such as Trifolium repens. Previous studies of acclimatization have been carried out in artificial experimental conditions, such as acrylic greenhouses or controlled environment chambers. The advent of FACE technology has enabled a large area of crop to be fumigated in the field, providing more realistic growing conditions. Pure stands of Trifolium repens L. cv. Blanca grown at either 355 or 600{mu}mol mol{sup -1} CO{sub 2} were examined, and their photosynthetic response to elevated Ca determined via gas exchange studies. Rates of photosynthesis of young, fully expanded leaves were increased between 21 and 36% when grown and measured at elevated CO{sub 2}. This increase in A corresponded to a decrease in g{sub S} of between 18 and 52%. No acclimation effect was observed in the most frequently cut stands, whilst the response of stands clipped only 4 times per year was more variable. When down regulation of V{sub cmax} did occur, this was not nearly as marked as that which occurred in 3 other temperate species (Chrysanthemum leucanthemum, Ranunculus friesianus, Plantago lanceolata (L.) J. & C. Presl.), at similar growth regimes. No acclimation of stomatal frequency, SI or pore length was found to occur in the enriched clover stands.

  12. Chemopreventive effects of standardized ethanol extract from the aerial parts of Artemisia princeps Pampanini cv. Sajabal via NF-?B inactivation on colitis-associated colon tumorigenesis in mice.

    PubMed

    Chung, Kyung-Sook; Choi, Hye-Eun; Shin, Ji-Sun; Cho, Eu-Jin; Cho, Young-Wuk; Choi, Jung-Hye; Baek, Nam-In; Lee, Kyung-Tae

    2015-01-01

    Chronic inflammation is an underlying risk factor of colon cancer, and NF-?B plays a critical role in the development of inflammation-associated colon cancer in an AOM/DSS mouse model. The aim of this study was to determine whether the standardized ethanol extract obtained from the aerial parts of Artemisia princeps Pampanini cv. Sajabal (EAPP) is effective at preventing inflammation-associated colon cancer, and if so, to identify the signaling pathways involved. In the present study, protective efficacy of EAPP on tumor formation and the infiltrations of monocytes and macrophages in colons of an AOM/DSS mouse model were evaluated. It was found that colitis and tumor burdens showed statistically meaningful improvements after EAPP administration. Furthermore, these improvements were accompanied by a reduction in NF-?B activity and in the levels of NF-?B-dependent pro-survival proteins, that is, survivin, cFLIP, XIAP, and Bcl-2. In vitro, EAPP significantly reduced NF-?B activation and the levels of IL-1? and IL-8 mRNA and pro-survival proteins in HT-29 and HCT-116 colon cancer cells. Furthermore, EAPP caused caspase-dependent apoptosis. Based on these results, the authors suggest EAPP suppresses inflammatory responses and induces apoptosis partly via NF-?B inactivation, and that EAPP could be useful for the prevention of colitis-associated tumorigenesis. PMID:25449198

  13. Effects of Grapevine Leafroll associated Virus 3 (GLRaV-3) and duration of infection on fruit composition and wine chemical profile of Vitis vinifera L. cv. Sauvignon blanc.

    PubMed

    Montero, R; Mundy, D; Albright, A; Grose, C; Trought, M C T; Cohen, D; Chooi, K M; MacDiarmid, R; Flexas, J; Bota, J

    2016-04-15

    In order to determine the effects of Grapevine Leafroll associated Virus 3 (GLRaV-3) on fruit composition and chemical profile of juice and wine from Vitis vinifera L. cv. Sauvignon blanc grown in New Zealand, composition variables were measured on fruit from vines either infected with GLRaV-3 (established or recent infections) or uninfected vines. Physiological ripeness (20.4°Brix) was the criterion established to determine the harvest date for each of the three treatments. Date of grape ripeness was strongly affected by virus infection. In juice and wine, GLRaV-3 infection prior to 2008 reduced titratable acidity compared with the uninfected control. Differences observed in amino acids from the three infection status groups did not modify basic wine chemical properties. In conclusion, GLRaV-3 infection slowed grape ripening, but at equivalent ripeness to result in minimal effects on the juice and wine chemistry. Time of infection produced differences in specific plant physiological variables. PMID:26675855

  14. Antioxidant and enzymatic responses to oxidative stress induced by pre-harvest water supply reduction and ripening on mango (Mangifera indica L. cv. 'Cogshall') in relation to carotenoid content.

    PubMed

    Rosalie, Rémy; Joas, Jacques; Deytieux-Belleau, Christelle; Vulcain, Emmanuelle; Payet, Bertrand; Dufossé, Laurent; Léchaudel, Mathieu

    2015-07-20

    The effects of a reduction in water supply during fruit development and postharvest fruit ripening on the oxidative status and the antioxidant defense system were studied in the mango fruit (Mangifera indica L.) cv. Cogshall. Changes in non-enzymatic (ascorbate) and enzymatic (SOD, CAT, APX, MDHAR, DHAR and GR) antioxidants, as well as oxidative parameters (H2O2 and MDA) and major carotenoids, were measured in unripe and ripe fruits from well-irrigated and non-irrigated trees. Under non-limiting water supply conditions, ripening induced oxidation as a result of the production of ROS and decreased ascorbate content. Antioxidant enzymatic systems were activated to protect fruit tissues and to regenerate the ascorbate pool. The carotenoid pool, mainly represented by ?-carotene and esterified violaxanthine isomers, accumulated naturally during mango ripening. The suppression of irrigation decreased fruit size and induced accumulation of ABA and of its storage form, ABA-GE, in fruit pulp from the earliest harvest. It also increased oxidation, which was observable by the high levels of ascorbate measured at the early stages at harvest, and by the delay in the time it took to reach the pseudo constant carotene-to-xanthophyll ratio in ripe fruits. Nevertheless, differences between the irrigation treatments on the antioxidant system in ripe fruits were not significant, mainly because of the drastic changes in this system during ripening. PMID:26232564

  15. Carbon balance, partitioning and photosynthetic acclimation in fruit-bearing grapevine (Vitis vinifera L. cv. Tempranillo) grown under simulated climate change (elevated CO2, elevated temperature and moderate drought) scenarios in temperature gradient greenhouses.

    PubMed

    Salazar-Parra, Carolina; Aranjuelo, Iker; Pascual, Inmaculada; Erice, Gorka; Sanz-Sáez, Álvaro; Aguirreolea, Jone; Sánchez-Díaz, Manuel; Irigoyen, Juan José; Araus, José Luis; Morales, Fermín

    2015-02-01

    Although plant performance under elevated CO2 has been extensively studied in the past little is known about photosynthetic performance changing simultaneously CO2, water availability and temperature conditions. Moreover, despite of its relevancy in crop responsiveness to elevated CO2 conditions, plant level C balance is a topic that, comparatively, has received little attention. In order to test responsiveness of grapevine photosynthetic apparatus to predicted climate change conditions, grapevine (Vitis vinifera L. cv. Tempranillo) fruit-bearing cuttings were exposed to different CO2 (elevated, 700ppm vs. ambient, ca. 400ppm), temperature (ambient vs. elevated, ambient +4°C) and irrigation levels (partial vs. full irrigation). Carbon balance was followed monitoring net photosynthesis (AN, C gain), respiration (RD) and photorespiration (RL) (C losses). Modification of environment (13)C isotopic composition (?(13)C) under elevated CO2 (from -10.30 to -24.93‰) enabled the further characterization of C partitioning into roots, cuttings, shoots, petioles, leaves, rachides and berries. Irrespective of irrigation level and temperature, exposure to elevated CO2 induced photosynthetic acclimation of plants. C/N imbalance reflected the inability of plants grown at 700ppm CO2 to develop strong C sinks. Partitioning of labeled C to storage organs (main stem and roots) did not avoid accumulation of labeled photoassimilates in leaves, affecting negatively Rubisco carboxylation activity. The study also revealed that, after 20 days of treatment, no oxidative damage to chlorophylls or carotenoids was observed, suggesting a protective role of CO2 either at current or elevated temperatures against the adverse effect of water stress. PMID:25462972

  16. The effect of supplementation with maize starch and level of intake of perennial ryegrass (Lolium perenne cv. Endura) hay on the removal of digesta from the rumen of sheep.

    PubMed

    Aitchison, E M; Gill, M; Osbourn, D F

    1986-09-01

    1. Eight wether sheep were offered a diet of perennial ryegrass (Lolium perenne cv. Endura) hay once daily at two levels of intake (11 and 16.5 g dry matter (DM)/kg live weight (LW] with or without maize starch (175 g DM/kg hay DM). The experiment consisted of four periods, each lasting 32 d. Rates of digestion of the hay were measured by incubation in dacron bags and rates of passage using chromium-mordanted hay. Rumen pool sizes of DM, organic matter and fibre were measured by emptying the rumen. 2. The inclusion of starch in the diet appeared to increase significantly (P less than 0.01) the lag phase before the start of fibre digestion, as observed both in the dacron bag studies and in the slower initial disappearance of digestible neutral-detergent fibre (NDF) from the rumen recorded by emptying of rumen contents. However, there was no significant effect of starch on apparent digestibility of fibre in the whole tract. 3. The main effect of increasing the level of feeding was to increase the rate of passage with a consequent decrease in overall digestibility. The fractional rate of passage increased from 0.0318 to 0.0400 as the level of feeding increased, while apparent digestibility of NDF decreased from 0.755 to 0.724. 4. On all treatments the weight of indigestible fibre in the rumen remained more or less constant between 5, 10 and 15 h after feeding, but was significantly lower at 24 h. These results suggest that a high proportion of the outflow of material from the rumen not associated with feeding appears to occur during the second half of the feeding cycle. PMID:3676226

  17. Thiabendazole uptake and storage performance of cactus pear [Opuntia ficus-indica (L.) Mill. Cv Gialla] fruit following postharvest treatments with reduced doses of fungicide at 52 degrees C.

    PubMed

    Schirra, Mario; Brandolini, Vincenzo; Cabras, Paolo; Angioni, Alberto; Inglese, Paolo

    2002-02-13

    The storage response of cactus pears [Opuntia ficus-indica Miller (L.) cv. Gialla] was investigated over 6 weeks at 6 degrees C, plus an additional week of simulated marketing period (SMP) at 20 degrees C, after a 3-min dip treatment with thiabendazole (TBZ) at 1000 mg/L at 20 degrees C or 150 mg/L TBZ at 52 degrees C. Untreated fruits were used as control. Following TBZ treatments at 20 and 52 degrees C, total residues were recovered from the peel of cactus pear, as the concentration of residues in the pulp was negligible. Treatments with 1000 mg/L TBZ at 20 degrees C resulted in a 2.82 mg/kg residue uptake (active ingredient, whole-fruit basis), whereas treatment at 150 mg/L TBZ left 1.09 mg/kg. TBZ showed great persistence over both storage and SMP: on average, in the fruits treated at 20 and 52 degrees C, over 72 and 68%, respectively, of TBZ was still present after SMP. Postharvest treatments with 1000 mg/L TBZ at room temperature did not affect the expression of slight-to-moderate chilling injury (CI), but reduced severe CI by approximately 50% and decay development by 63.4% in comparison to those of untreated fruit after SMP. The effectiveness of TBZ was much higher with the treatment at 150 mg/L TBZ at 52 degrees C, providing 91% control of severe CI and approximately 89% suppression of decay; no treatment damage occurred during storage and SMP. External appearance was better in fruit treated with 150 mg/L TBZ at 52 degrees C. Respiration rate, titratable acidity, soluble solids concentration, and acetaldehyde in the flesh were not significantly influenced by treatments. Ethylene production rate and ethanol levels in the flesh were significantly higher in the TBZ-treated fruit as opposed to those in the untreated control fruit. PMID:11829638

  18. Dr. Canbing Li CV Personal Information

    E-print Network

    Sino-Italy Renewable and Efficiency Advanced Training Program (hosted by Ministry of Science of China Secretary of Experts Panel in Advanced Energy (part-time) ·Nov. 2011- present Hunan University, Yonghong Kuang, Yi Tan. Comprehensive review of renewable energy curtailment and avoidance: a specific

  19. Alexandra Main CV 1 ALEXANDRA MAIN

    E-print Network

    Oviedo, Néstor J.

    press). Associations of parent-adolescent relationship quality with type 1 diabetes management academic achievement of Chinese American children in immigrant families. Early Child Research Quarterly expression in the family: Relations with parents' cultural orientations and children's emotion

  20. David B. Senn -CV CURRICULUM VITAE

    E-print Network

    masters thesis committee member 1998,2001,2003,2006 Orleans Parish Public Schools, New Orleans LA Teacher biogeochem.) Eawag Seed Funding proposal, Measuring N isotopes in specific amino acids to accurately quantify

  1. Mengistu CV 1 Dr. Tesfamariam Mekete Mengistu

    E-print Network

    Jawitz, James W.

    of Bonn, Germany. Dissertation: "Presence and frequency of occurrence of plant parasitic nematodes parasitic nematodes associated with plants used for biofuels. March 2006: Visiting scientist to renowned Distinction. Thesis: "Damage potentials of root-knot nematode, Meloidogyne incognita and Meloidogyne javanica

  2. NASA/ESA CV-990 spacelab simulation

    NASA Technical Reports Server (NTRS)

    Reller, J. O., Jr.

    1976-01-01

    Simplified techniques were applied to conduct an extensive spacelab simulation using the airborne laboratory. The scientific payload was selected to perform studies in upper atmospheric physics and infrared astronomy. The mission was successful and provided extensive data relevant to spacelab objectives on overall management of a complex international payload; experiment preparation, testing, and integration; training for proxy operation in space; data handling; multiexperimenter use of common experimenter facilities (telescopes); multiexperiment operation by experiment operators; selection criteria for spacelab experiment operators; and schedule requirements to prepare for such a spacelab mission.

  3. Malus domestica cv. Golden Delicious (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    stream_source_info A golden opportunity.pdf.txt stream_content_type text/plain stream_size 9043 Content-Encoding ISO-8859-1 stream_name A golden opportunity.pdf.txt Content-Type text/plain; charset=ISO-8859-1 tx H2O... | pg. 20 cientists at three Texas universities investigating golden algae, its explosive growth, and its deadly toxins have dis- covered an apparent competition between golden algae and blue green algae in certain Texas lakes. Understanding...

  4. Ichinose CV 1 Megan C. Ichinose

    E-print Network

    Park, Sohee

    , pre-medical studies RESEARCH EXPERIENCE August 2013 Park Clinical Neuroscience Lab ­ Vanderbilt: Examining deficits or abnormalities in attention and memory processes as- sociated with schizophrenia and their relationship to positive and negative symptomology. September 2011 Belief, Learning & Memory Lab ­ Yale School

  5. Young.CV.2014 CURRICULUM VITAE

    E-print Network

    Hanley, Kathryn A.

    in the identification of algae strains to be used as alternative fuel sources. - Implemented algae culture nutrient data collation and analysis utilizing colormetric assays for ion detection. - Performed QPCR on algae culture

  6. Xin Lai, CV Page 1 Curriculum Vitae

    E-print Network

    Wolkenhauer, Olaf

    and Administration Experience 2009-2013 Hands on Lectures in Systems Biology I and II miRNA regulation of gene and Administrator for BMBF-Funded Research Project CALSYS www.sbi.uni-rostock.de/calsys 2011 A Practical Lecture Research, in press, 2013. A. Sittka, J. Vera, X. Lai, B. Schmeck. Systems Biology of asthma. Pediatric

  7. Bougainvillea x buttiana cv. Louis Wathen (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    Drawing on newspaper, movement correspondence, and interview data, I examine the tactical selection and (in)visibility of a lesbian and gay student group, Gay Student Services (GSS), in a hostile university campus in Texas from the mid-1970s through...

  8. Echinacea purpurea cv. White Swan (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    In this dissertation we examine some of the challenges faced by the congestion control algorithms of TCP in emerging networks. We focus on three main issues. First, we propose TCP with delayed congestion response (TCP-DCR), ...

  9. Lagenaria siceraria cv. Mexican Bottle (Cultivated) 2 

    E-print Network

    Hugh D. Wilson

    2011-08-10

    purification system, called Flue Gas Desulfurization (FGD). Abatement of these waste products is a growing concern, not only for the industry, but the environment as well. It is possible to produce a gypsum brick unit that can meet the engineering properties...

  10. CV OF CHANGQING LI CHANGQING LI

    E-print Network

    Oviedo, Néstor J.

    Society of Nuclear Medicine Molecular Imaging Postdoctoral Award 2010 Paper of "Cerenkov luminescence," by Manoj Mathew. 2012 Alavi Mandell Award from Society of Nuclear Medicine JOURNAL PAPERS/INVITED BOOK and multispectral 3-dimensional fluorescence optical tomography imaging system", J. Nuclear Medicine, 52, 1268

  11. Veronica sp cv. Sunny Border Blue (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    mechanisms of ROS-induced neuronal damage by paraquat???..44 10 Effect of paraquat on trypan blue exclusion??????????????64 11 Effect of paraquat on formazan reduction??????????????...65 12 Effect of paraquat on LDH release... Page II MATERIALS AND METHODS.?????????????...............48 Sources of materials????????????????????.. 48 Cell culture and chemical treatment??????????????..49 Trypan...

  12. CV -Rhee -1 1 Jinny Rhee, Professor

    E-print Network

    Su, Xiao

    for Improving Thermal Stratification," accepted for publication in ASME J Solar Energy Engineering; also from Thermal Diodes in Solar Hot Water Storage Tank," Solar Energy, Vol. 84, pp. 507-511 8. Rhee, J, "Numerical Optimization of a Cogenerating Parabolic Solar Collector Receiver," proce

  13. Malus domestica cv. Granny Smith (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    from that of Golden Delicious in being less tart yet acceptable and eye ap peal is good. Granny Smith is a standard variety with round green fruit. Flavor appears to be outstand ing. Observational Varieties This list includes varieties....1294 Extension Service? The Texas A&M University System? Daniel C. Pfannstiei. Director? College Station, Texas texas apples B1294 CONTENTS Soi I. . . . . . . . . . . . . . . . . . . . . . . .. 3 Orchard Establ ishment. . . . .. 3 Varieties...

  14. Malus domestica cv. Pink Lady (Cultivated) 

    E-print Network

    Monique D. Reed

    2011-08-10

    Floyd-Wilson. English Ethnicity and Race in Early Modern Drama. New York: Cambridge University Press, 2003. xii + 256 pp. + 10 illus. $65.00. Review by JONATHAN BURTON, WEST VIRGINIA UNIVERSITY. Even for experienced readers of early modern texts... of physiological differences. English Ethnicity and Race in Early Modern Drama by Mary Floyd-Wilson extends Paster?s project by arguing that regionally inflected humoralism, or ?geo- humoralism,? was ?the dominant mode of ethnic distinctions in the late sixteenth...

  15. CV Name: Jianjun Yang JIANJUN YANG

    E-print Network

    Sparks, Donald L.

    collaborative carbon project, aimed at soil carbon sequestration for climate change mitigation. Besides Prof in agricultural ecosystem, thus being of great importance for food security and carbon sequestration. Synchrotron understanding of SOM stability and carbon sequestration in soil systems. Peer-reviewed Publications 1. Yang J

  16. Liming Zhou CV for Liming Zhou

    E-print Network

    Zhou, Liming

    , Boston University, USA, 2002 Warming enhanced plant growth in the north since the 1980s: A greener and for NSF and NASA proposals. Before coming to US, I was a weather forecaster (1994-1998) at National: The View from Space, edited by King, M., Parkinson, C., Partington, K.C., and Williams, R.G., Cambridge

  17. Capsicum annuum cv. Poblano type (Cultivated) 

    E-print Network

    Monique D. Reed

    2011-08-10

    different type of polyphenolic compound was assessed. Transport of gallic acid was enhanced by the use of these agents that cemented the idea that phospholipids and terpenes can enhance the transport of various types of polyphenolics. The aiding effect...

  18. Naomi Ellemers brief CV information February 2010

    E-print Network

    making, discrimination and career development, diversity and innovation, social justice and morality identity, collective action, intergroup aggression, innovation at work, in the Netherlands (University;Committee), ethnic and gender integration vs. segregation in educational and work settings (NWO), family

  19. V479 And: CV, LMXB, or Symbiotic?

    NASA Astrophysics Data System (ADS)

    Buitrago, Diego González; Tovmassian, Gagik; Echevarría, Juan; Zharikov, Sergey; Miyaji, Takamitsu; Avilés, Andres; Valyavin, Gennady

    2013-01-01

    V479 And is a 14.26 hour, close binary system, comprised of a G8-K0 star departing from the main sequence and a compact primary star accreting matter from the donor. The object is an X-ray source, modulated with the orbital period. This, and the presence of an intense He II line, leads us to speculate that the compact object is a magnetic white dwarf. However, we do not find strong constraints on the upper mass limit of the compact object, and we may have a neutron star in a low mass X-ray binary instead of a cataclysmic variable. The orbital period is certainly too short for the donor star to be an evolved giant star, so classifying this object as a symbiotic binary may be a big stretch; however there is an evidence that the mass transfer occurs via stellar winds, rather than through the L1 point of Roche filling secondary, a phenomenon more common for symbiotic stars.

  20. Mandevilla sanderi cv. Scarlet Pimpernel (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    Proventricular dilatation disease (PDD) was first recognized during an outbreak among captive macaws in the late 1970s. The disease, also known as proventricular dilatation syndrome or macaw wasting disease can occur in any psittacine but the most...

  1. CV of Q. X. Huang Curriculum Vitae

    E-print Network

    -2006, Experiment Supervisor, "Velocity measurement of Bunsen burner flame front propagation based on image-intrusive three-dimensional temperature measurement for combustion system Combustion optimization using flame processing technology", Flame Image Processing lab in State Key Laboratory of Clean Energy Utilization

  2. Petunia x hybrida cv. California Giant (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    or diffusion of dissolved 230Th from the oligotrophic North Pacific gyre (low particle flux) to the more productive equatorial region (high particle flux), and (2) the efficacy of using 232Th – 230Th concentration systematics as a proxy for estimating dust...

  3. Page 1 of 20 David Vine CV

    E-print Network

    Carlini, David

    , the World's Most Dangerous Place. In Bioinsecurity and Vulnerability. Lesley Sharp and Nancy Chen, eds. Pp and Struggle for Identity Against Two World Powers. Sandra J.T.M. Evers and Marry Kooy, eds. Pp. 11-124. In Evers and Kooy. 2011 Sorrow, Sadness, and Impoverishment: The Lives of Chagossians in Mauritius. 2nd

  4. CONCURSO 5U CV STARTUP (II EDICIN)

    E-print Network

    Escolano, Francisco

    Universidad de Alicante se ampara en el marco del programa "Campus del Emprendedor Innovador" financiado por 26 de diciembre. El programa "Campus del Emprendedor Innovador" pretende auspiciar el talento emprendedor en los campus de las 5 universidades públicas valencianas con el objetivo de generar nuevos

  5. Bougainvillea cv. San Diego Red (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    stream_source_info WEHRLY-DISSERTATION-2015.pdf.txt stream_content_type text/plain stream_size 103720 Content-Encoding UTF-8 stream_name WEHRLY-DISSERTATION-2015.pdf.txt Content-Type text/plain; charset=UTF-8... THE EFFECT OF CHANGES IN TEACHER-STUDENT RELATIONSHIP QUALITY ON STUDENTS’ TRAJECTORIES FOR INTERNALIZING BEHAVIORS A Dissertation by SARAH ELIZABETH WEHRLY Submitted to the Office of Graduate and Professional Studies of Texas A&M University...

  6. Malus domestica cv. Red Delicious (Cultivated) 

    E-print Network

    James R. Manhart

    2011-08-10

    subjected to 4 tests: (1) no incentive to cross; (2) extra food and water incentive to cross; (3) fawn separated from doe; and, (4) estrous doe separated from mature buck. Three deer guard designs tested included: (1) a guard installed at ground level; (2...

  7. Begonia rex-cultorum cv. Lalomie (Cultivated) 

    E-print Network

    David C. Reed

    2011-08-10

    During the summer of 2000, twenty teachers came from rural schools throughout Texas to take part in the Lone Star Dinosaur Field Institute. Hosted by the professional developers at the Fort Worth Museum of Science and History, the Institute is a...

  8. Lippert CV Page 1 Peter C. Lippert

    E-print Network

    Johnson, Cari

    .Y. Li, P.C. Lippert, T.S. Yang, X.X. Zhao, J. Fu, Y. Yue. 2015. Late Triassic paleolatitude.C. Lippert, D.J.J. van Hinsbergen, M.J. Dekkers, Z.J. Guo, R. Waldrip*, M. Ganerød, X.C. Li, and P. Kapp. Waldrip*, X.C. Li, X.R. Zhang, D.D. Liu, and P. Kapp, 2015. Can a primary remanence be retrieved from

  9. CV A. Tatarenkov Page 1 CURRICULUM VITAE

    E-print Network

    Chen, Zhongping

    @uci.edu EDUCATION: 1982-1987 B.Sc. in Biology, Far-Eastern State University, (Vladivostok, Russia). February, 1995 Ph.D. in Genetics, St. Petersburg State University (St. Petersburg, Russia). RESEARCH INTERESTS systems, sexual selection, microbial ecology, evolution of drug resistance. WORKING EXPERIENCE: 1987

  10. Hynek CV, Page 1 CURRICULUM VITAE

    E-print Network

    Mojzsis, Stephen J.

    of Geological Sciences and Research Associate at the Laboratory for Atmospheric and Space Physics 392 UCB in the Laboratory for Atmospheric and Space Physics (LASP), both at the University of Colorado. His recent research at the Laboratory for Atmospheric and Space Physics, University of Colorado, 2003-present Characterized

  11. CV Siobhan Stevenson Page 1 of 10

    E-print Network

    . Canadian Journal of Information and Library Science, 32(1/2), 1 -34. Stevenson, S. (2007). Public libraries: A textually oriented discourse analysis. Canadian Journal of Information and Library Science, 26(2/3), 50

  12. Codiaeum variegatum cv. Jan Bier (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    -equivalent occurs in utero (as opposed to post-natal as occurs in the rodent). It is known that both maternal and fetal cortisol levels increase in response to alcohol. The role of cortisol in mediating fetal cerebellar Purkinje cell loss (known to occur... reduce CA1 pyramidal cell number. Interestingly, we recently reported that the estimated number of cerebellar Purkinje cells were reduced in response to all three trimester- equivalent alcohol exposure (BAC, 189 mg/dl) in the sheep model (Ramadoss et...

  13. C. Karunanithy CV 1 KARUNANITHY CHINNADURAI

    E-print Network

    Wu, Mingshen

    of flavored malted beverages Energy accounting and auditing of food industries Development of HACCP plan, and algae production for biofuel. Position: Graduate Research Assistant, Graduate Teaching Assistant: Optimizing extruder and biomass parameters for maximum fermentable sugar yield toward bio-fuels production

  14. Malus domestica cv. Pacific Rose (Cultivated) 

    E-print Network

    Monique D. Reed

    2011-08-10

    residential, where consumption is less than 150,000 cubic feet. When customers exceed that usage level they are charged an applicable large volume rate. Entex purchases the natural gas distributed to the City of La Grange from Pacific Gas and Electric...-1 Section 4 Housing City of La Grange 4-1 Comprehensive Plan 2020 HOUSING This section of the Comprehensive Plan provides a guide for developing housing resources in La Grange. It is composed of two subsections: (1) Existing Housing and (2...

  15. CV Ada Ortiz 1 CURRICULUM VITAE

    E-print Network

    Sanahuja, Blai

    ). January 2001 - December 2002. · ESA (European Space Agency) Fellowship to work under the project "An Space. I, II and III Sponsoring Agency: European Space Agency (ESA) PI: Dr. B. Sanahuja (University space projects. Sponsoring Agency: Ministerio de Ciencia y Tecnolog´ia (PNAA Program), Spain PI: Dr. B

  16. Resistance of tomato genotypes to the greenhouse whitefly Trialeurodes vaporariorum (West.) (Hemiptera: Aleyrodidae).

    PubMed

    Lucatti, Alejandro F; Alvarez, Adriana E; Machado, Cristina R; Gilardón, Elsa

    2010-01-01

    The greenhouse whitefly, Trialeurodes vaporariorum Westwood, is the most common and abundant whitefly in Argentine horticultural greenhouse crops, especially in tomato (Solanum lycopersicum). Resistance in some wild tomato relatives, such as S. peruvianum, S. habrochaites and S. pennellii to the greenhouse whitefly has been described. The Mi gene confers effective resistance against several species of insects, among them the sweet potato whitefly, Bemisia tabaci Gennadius. Resistance to T. vaporariorum was found in the prebreeding line FCN 93-6-2, derived from a cross between S. lycopersicum cultivar Uco Plata INTA (MiMi) and the wild line FCN 3-5 S. habrochaites. The purpose of this study was to evaluate resistance to T. vaporariorum in tomato genotypes and to study the relationship between this resistance and the presence of the REX-1 marker, which is linked to the Mi gene. In a free-choice assay, the average number of adults per leaf and the number of immatures on the middle and basal plant parts were analyzed. In a no-choice assay, the oviposition rate and adult survival rate were calculated. For all variables analyzed, FCN 3-5 was the most resistant strain. Variations were found in the F2 progeny between the prebreeding line FCN 13-1-6-1 and cv. Uco Plata INTA. Results from the F2 progeny indicate that resistance to T. vaporariorum may be polygenic with transgressive segregation. Whitefly resistance was found to be independent of the REX-1 marker. PMID:21120390

  17. Cytogenetic studies in four cultivated Amaranthus (Amaranthaceae) species

    PubMed Central

    Bonasora, Marisa Graciela; Poggio, Lidia; Greizerstein, Eduardo José

    2013-01-01

    Abstract In the present study, the chromosomes numbers were confirmed, 2n = 34 for Amaranthus cruentus Linnaeus, 1759, and 2n = 32 for Amaranthus hypochondriacus Linnaeus, 1753, Amaranthus mantegazzianus Passer, 1864, and Amaranthus caudatus Linnaeus, 1753. The distribution and variability of constitutive heterochromatin were detailed using DAPI-CMA3 banding technique. The position of the nucleolus organizer region (NOR) was observed using Ag-NOR banding (active loci) and fluorescent in situ hybridization (rDNA-FISH) in the four Amaranthus species. Variations in the amount of constitutive heterochromatin were detected both within the species and between them, with DAPI-CMA3 stain. One chromosome pair having a NOR was found in each studied accession, with exception of Amaranthus caudatus cv. EEA INTA Anguil. This accession presented four rDNA loci (FISH), being active two of them (Ag- banding). PMID:24260690

  18. A.T. Kramer CV 1 Andrea T. Kramer

    E-print Network

    Wagenius, Stuart

    of Illinois at Chicago Thesis advisor: Mary V. Ashley Dissertation Title: Ecological genetics of Penstemon seed viability in prairie forbs: a test of three methods Native Plants Journal (in press) Barak, R. S. Assessing potential seed transfer zones for five forb species in the Great Basin. Natural Areas Journal 35

  19. ES9 NPP-FM5 Ed1-CV

    Atmospheric Science Data Center

    2015-11-12

    ... Detailed CERES ERBElike Level 3 (ES-4/ES-9) Product Information Collection Guide:  ES9 CG R1V1  (PDF) Data ... for instrument validation purposes only and not suited for science publications. SCAR-B Block:  ...

  20. Dantzer CV Page 1/7 DR. BEN DANTZER A

    E-print Network

    , Conservation Physiology, Functional Ecology · Mentored 35 undergraduate students in field and laboratory 2013-October 2014 · Director of Studies in Natural Sciences, Churchill College, Cambridge, Oct. 2012 in Biology, Intersections of Biology and Mathematics, "Unifying modeling and empirical approaches

  1. HARVARDgazette17-30 SEPTEMBER 2009 VOL. CV NO. 2

    E-print Network

    Wolfe, Patrick J.

    , and astronomy learn a trick or two from video games and in- vestigating a new kind of computing based.harvard.edu/gazette/story/ 2009/09/longfellow-online-exhibition/ WIND COULD BE CHINA'S ANSWER A team of environmental scientists-generated electricity in China. news.harvard.edu/gazette/story/ 2009/09/china-energy-needs-wind/ Christine Heenan Vice

  2. James F. Gilchrist cv -page 1/18

    E-print Network

    Gilchrist, James F.

    Articles 37) X. Li and J. F. Gilchrist, "Large-area Nanoparticle Films by Continuous Automated Langmuir- Blodgett Assembly and Deposition" submitted to Langmuir. 36) K. Joshi, T. Muangnapoh, M. D. Stever, and J. F. Gilchrist, "Effect of ionic strength and surface charge on convective deposition", submitted

  3. Pritzker School of Medicine Sample CV HOME ADDRESS SCHOOL ADDRESS

    E-print Network

    Sherman, S. Murray

    School of Medicine Chicago, IL zip 924 E. 57th St., Room 104 Phone # Chicago, IL 60637-5415 Email (773 (Quality and Safety Scholarship track). 2010-2012 Northwestern University, John Black, PhD; "Tracking avian of Hospital Medicine Conference; Chicago, IL. Doe, J. (2011) Tracking avian flu pandemics. Northwestern

  4. Brinker Master CV_Oct2015 C. JEFFREY BRINKER

    E-print Network

    Brinker, C. Jeffrey

    Genetics and Microbiology, Co- Director Center for Micro-Engineered Materials The University of New Mexico 2008 - current: Distinguished Professor of Chemical and Biological Engineering and Molecular Genetics and Biological Engineering and Molecular Genetics and Microbiology, UNM. Co-developed new graduate curriculum

  5. David Keith, Academic CV (Feb-13) Page 1 David Keith

    E-print Network

    Chen, Yiling

    of geoengineering. In 1990 I wrote one of the first comparative technology and policy assessments of geoengineering few years I have organized interdisciplinary meetings on geoengineering, created an annual summer

  6. 1 KS.Inglett_CV KANIKA SHARMA INGLETT

    E-print Network

    Balser, Teri C.

    University of Delhi, New Delhi, India, 1997. M.S., Biochemical Technology, University of Delhi, New Delhi title. Generation of antibodies against antigens of Mycobacterium tuberculosis using hybridoma technology. TEACHING Soil and Water Science Department, University of Florida Instructor, Bioremediation

  7. Olson CV, June 2015 Peter Brock Olson, Ph.D.

    E-print Network

    Wu, Mingshen

    in American literature ABD University of Memphis, Musicology/Southern Regional Music, 2003 M.A. University for American Music. 2.1 (2008): 113-116. "An Appalachian Ballad." Writing About Identity in the South. Ed. Keller, et al. New York: McGraw- Hill, 1994. 102-3:10-14. "Three Appalachian Ballads." Crossroads

  8. ES8 NPP-FM5 Ed1-CV

    Atmospheric Science Data Center

    2015-11-16

    ... Detailed CERES ERBElike Level 2 (ES-8) Product Information Collection Guide:  ES8 CG R3V4  (PDF) Data ... for instrument validation purposes only and not suited for science publications. SCAR-B Block:  ...

  9. ES4 NPP-FM5 Ed1-CV

    Atmospheric Science Data Center

    2015-11-16

    ... Detailed CERES ERBElike Level 3 (ES-4/ES-9) Product Information Collection Guide:  ES4 CG R1V1  (PDF) Data ... for instrument validation purposes only and not suited for science publications. Additional Info:  f SCAR-B ...

  10. Hallas CV Page 1 of 8 Roger Hallas

    E-print Network

    Raina, Ramesh

    401 Hall of Languages Office: 315-443-4947 Syracuse, NY 13244 Fax: 315-443-3660 EDUCATION: Ph.D by Deb Waterhouse- Watson) Senses of Cinema, no. 62 (2012) (review by Joseph Valle) Screen 53, no. 1 2010): 44 (review by James Polchin) A&U: America's AIDS Magazine (November 2010) (review by Chael

  11. Hibiscus rosa-sinensis cv. Lemon Chiffon (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    and industrial wastewaters gen- erally has higher salt levels. What problems can salty water cause? S alty irrigation water can cause two major problems in crop pro- duction?salinity hazard, Mark McFarland, Robert Lemon and Charles Stichler* Critical Salt Levels... Crops MEASUREMENT PEANUTS CORN GRAIN SORGHUM COTTON Sodium Adsorption Ratio (SAR) No units (just a number) 10 10 10 10 Total Dissolved Salts (Electrical Conductivity or Total Dissolved Solids*) Micromhos per centimeter (umhos.cm) 2100 1100 1700 5100...

  12. The Missing CV Population: Results From An Objective Prism Survey

    E-print Network

    C. Tappert; T. Augusteijn; J. Maza

    2001-09-28

    We present the results of a search for cataclysmic variables (CVs) in the Calan-Tololo survey. We detected a total number of 21 CVs, 12 of them are previously unknown objects. Our results suggest that the mismatch between the theoretically predicted sample and the observed one is not due to observational bias but has to be resolved by a revision of the theoretical models.

  13. HARVARDgazette01-14 APRIL 2010 VOL. CV NO. 13

    E-print Network

    Wolfe, Patrick J.

    - vard and China: A Research Symposium," a series of lectures, panels, and break-out sessions held nonprofit environmental group, announces plans to move its headquarters and staff to a Harvard- owned

  14. HARVARDgazette04-17 MARCH 2010 VOL. CV NO. 11

    E-print Network

    Wolfe, Patrick J.

    Rollins Senior Multimedia Producer Max Daniels Web Specialist Jennifer Hodge Web Content Producer's research takes him far from the Yard, trolling for deep-sea life in the North Atlantic. Page 4 Harvard Christopher Traganos Web Developer Distribution and Subscriptions: 617.495.4743 Delivered free to faculty

  15. Quantifying the CV: Adapting an Impact Assessment Model to Astronomy

    NASA Astrophysics Data System (ADS)

    Bohémier, K. A.

    2015-04-01

    We present the process and results of applying the Becker Model to the curriculum vitae of a Yale University astronomy professor. As background, in July 2013, the Becker Medical Library at Washington Univ. in St. Louis held a workshop for librarians on the Becker Model, a framework developed by research assessment librarians for quantifying medical researchers' individual and group outputs. Following the workshop, the model was analyzed for content to adapt it to the physical sciences.

  16. NASA/ESA CV-990 Spacelab Simulation (ASSESS 2)

    NASA Technical Reports Server (NTRS)

    Mulholland, D. R.; Androes, G. M.; Reeves, J. F.

    1978-01-01

    To test the validity of the ARC approach to Spacelab, several missions simulating aspects of Spacelab operations have been conducted as part of the ASSESS Program. Each mission was designed to evaluate potential Shuttle/Spacelab concepts in increasing detail. For this mission, emphasis was placed on development and exercise of management techniques planned for Spacelab using management participants from NASA and ESA who have responsibilities for Spacelab 1 which will be launched in 1980.

  17. Revised 1/2009 SAMPLE JOHN DOE CV & GRANTS

    E-print Network

    Bushman, Frederic

    Therapy, University of Pennsylvania School of Medicine Other Appointments: 1999-present Senior Fellow of Respiratory Diseases 2005- present Reviewer ­ Annals of Internal Medicine Academic and Institutional" - National Respiratory Association, San Diego, California Organizing Roles in Scientific Meetings: April 19

  18. CV of Monica Pokorny page 1 MONICA L. POKORNY

    E-print Network

    Maxwell, Bruce D.

    and stream ecological restoration projects including planning and priority setting, NEPA compliance to quantify water movement into and through the ash pond cap system. Wildlife Habitat Restoration Ecologist). Monitored rangeland health on reclaimed lands and conservation easements. Conducted inventories

  19. Early Phases of High Mass Transfer Rate During CV Evolution

    NASA Astrophysics Data System (ADS)

    Schenker, K.

    It is likely that some binary systems consisting of a white dwarf (WD) primary and a main sequence companion are formed with a mass ratio exceeding the critical value for stable mass transfer. The case studied here occurs if the secondary star fills its Roche lobe while still on the main sequence. Various aspects of stability issues are presented as well as results of recent numerical evolution calculations. The violation of the thermal stability criterion leads to the expectation of thermal-timescale mass transfer. Such a scenario has been invoked to explain supersoft X-ray sources (SSS) and WDs growing in mass and thus potentially leading to SN Ia. A crucial parameter is the accretion efficiency ?, defined as the fraction of the transferred mass that finally remains on the WD. To improve on ad hoc assumptions for ?, tabulated results from nova outburst computations (Kovetz & Prialnik, 1995) and optically thick wind models (Kato, 1997) are included in evolutionary calculations. Current results show that it seems to be rather difficult to reach Chandrasekhar mass in this scenario (unless already starting with a very massive WD). Although the SSS phase in such systems can actually reach lower orbital periods than might be expected, the shortest period SSSs are probably of different origin. Furthermore various unusual CVs (such as AE Aqr) are to be understood as consequences of a thermal-timescale mass transfer history and can now be modelled in considerable detail.

  20. Chris Summa. CV. 17 January 2013 1 Christopher M. Summa

    E-print Network

    Summa, Christopher M.

    .S. (with Honors) in Biochemistry and B.A. in Russian Senior Thesis: "A Search for Isozymes of Glutamine Synthetase in Human Brain" Experience 2007 - present Assistant Professor of Computer Science University

  1. CV Prof A. Piccolo 1 Prof. Antonio Piccolo Curriculum vit

    E-print Network

    Costagliola, Gennaro

    management of power flows in electrified systems, ICT applications on embedded GD, clean energy, soft is the chairman of the Scientific Board of "Smart Power System", High Technology District of Campania Region been Full Professor of "Power System" and "Electrical Plants, Machines and Drives" at the Faculty

  2. CV: Hlusko, 18 January 2013, page 1 LESLEA J. HLUSKO

    E-print Network

    Hlusko, Leslea J.

    1995 ­ 1996 Internship, National Museum of Natural History, Smithsonian Institution (Dr. R. Potts of Medicine, Hershey, PA 1996 ­ 1997 Graduate Research Assistant, Dept. Anthropology, Penn State University of Anthropological Genetics RESEARCH AFFILIATIONS 2005 ­ pres Research Associate, Cleveland Museum of Natural History

  3. CV & LIST OF PUBLICATIONS Prof. Dr. phil. Christoph Gradmann

    E-print Network

    Laudal, Arnfinn

    ) PUBLICATIONS MONOGRAPHS: - Laboratory Disease. Robert Koch's Medical Bacteriology, Baltimore: John's Hopkins University Press 2009, 318 pp. - Krankheit im Labor. Robert Koch und die medizinische Bakteriologie

  4. HARVARDgazette04-17 FEBRUARY 2010 VOL. CV NO. 9

    E-print Network

    Wolfe, Patrick J.

    Hasty Pudding's Woman of the Year Anne Hathaway (left) was toasted last week in a parade through Harvard AT COOKING "There's this huge fantastic mystery: Where did humans come from? I think we came out

  5. Susan Blake CV 1 SUSAN BLAKE CURRICULUM VITAE

    E-print Network

    Indiana University

    Fellowship, Indiana University Philosophy Department, 2011-2012. Bo and Lynn Clark Memorial Prize, that perceptions and beliefs with such content are sometimes false, as Sylvia takes hers to have been. Sylvia perception to be false. Her capaci

  6. CV, A.J. Reisinger 1 CURRICULUM VITAE

    E-print Network

    : E.J. Rosi-Marshall and P.M. Groffman 2010- 2015 Graduate Research Assistant, Dept. of Biological.L. Tank, E.J. Rosi-Marshall, R.O. Hall, and M.A. Baker. In review (Biogeochemistry). The varying role.L., R.O. Hall, E.J. Rosi-Marshall, M.A. Baker, M. Sivapalan, S. Ye and A.J. Reisinger. Rivers

  7. OpenCV and TYZX : video surveillance for tracking.

    SciTech Connect

    He, Jim; Spencer, Andrew; Chu, Eric

    2008-08-01

    As part of the National Security Engineering Institute (NSEI) project, several sensors were developed in conjunction with an assessment algorithm. A camera system was developed in-house to track the locations of personnel within a secure room. In addition, a commercial, off-the-shelf (COTS) tracking system developed by TYZX was examined. TYZX is a Bay Area start-up that has developed its own tracking hardware and software which we use as COTS support for robust tracking. This report discusses the pros and cons of each camera system, how they work, a proposed data fusion method, and some visual results. Distributed, embedded image processing solutions show the most promise in their ability to track multiple targets in complex environments and in real-time. Future work on the camera system may include three-dimensional volumetric tracking by using multiple simple cameras, Kalman or particle filtering, automated camera calibration and registration, and gesture or path recognition.

  8. C.V Keif Oss ossk@uwstout.edu

    E-print Network

    Wu, Mingshen

    , Graphic Communications, Graphics Software Tools, Post-Press Operations Associate architecture, website design, HTML and CSS programming, search engine optimization, photo post-processing, flash photo gallery creation and content writing. #12

  9. Shengli Chen, CV 1/5 Shengli Chen

    E-print Network

    Cai, Steve C. S.

    of Applied Mathematics Journal of Petroleum Science and Engineering International Journal of Oil, Gas and wellbore stability Hydraulic fracturing EDUCATION Ph.D. Petroleum Engineering (Geomechanics Petroleum Engineer Houston, TX 02/2013-08/2014 Integrated PoroMechanics Institute Research Associate

  10. CV, Nihad Bunar Professor of Child and Youth Studies

    E-print Network

    Wohlfarth, Barbara

    for the research program "Urban renewal policies and their effects in Stockholm's segregated areas". 2003 400 public lectures and presentations, related to my research on young people, multiculturalism, urban

  11. Harp CV p.1 of 5 Elizabeth (Liz) Mari Harp

    E-print Network

    Anderson, Charles W.

    research integrates parasitology, evolutionary biology, and ecological genetics to investigate parasite Dissertation Improvement Grant $14,476 2008 American Society of Veterinary Parasitologists Travel Grant $500

  12. Hudman CV -1/6 Rynda C. Hudman

    E-print Network

    Hudman, Rynda

    and agricultural soil nitric oxide emissions using surface and satellite observations and air pollution modeling for Atmospheric Research (NCAR), 1999-2001. SKILLS Air pollution modeling: GEOS-Chem model; GCAP chemistry, aircraft, and satellite air pollutant observations; statistical regressions of meteorological and land

  13. Hibiscus rosa-sinensis cv. Iani Booth (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    , 1876) Price, E. W. , 1940a, 76-78,fig. lA-C(tod)(syn. :Qncho- cotyle emarginata Olsson, 1876,0. appenr- d i ? u 1 a t a Sonsino, 1891 ??? Kuhn, 1829) (Raja clavata; gills; Plymouth,England); 1942a, 53. - -Polianskii, 1.1. , 1955a, 41(R. radiata, R...?cola dupotetiana, Alytes ob- stetricans, Rana temporaria, R. esculenta, Amblystoma mexicanum, Cerastes cor- nutus mutila, Viper lebe tina ;Alger);l 953 b. 13,14,15, 19-23;1955a,270-271,fig. 3. joyeuxi inexpectata B?ttner, ?., 1951c, 155, 157,fig. 51 (Zamenis...

  14. Properties of NAC and CV[NAC] for Energy Models 

    E-print Network

    Ruch, D. K.

    1993-01-01

    stream_source_info ESL-TR-11-11-01.pdf.txt stream_content_type text/plain stream_size 12152 Content-Encoding UTF-8 stream_name ESL-TR-11-11-01.pdf.txt Content-Type text/plain; charset=UTF-8 Energy Systems Laboratory p...-Cevantes, Sung Lok Do, Kee Han Kim, Cyndi Lewis, Bahman Yazdani – Energy Systems Laboratory James Yarborough, U.S.E.P.A. ESL-TR-11-11-01 Energy Systems Laboratory p. 2 Why care about energy efficiency-renewable energy in schools? • Lower energy costs...

  15. CURRICULUM VITAE Yin Tang CV Pages 1/2

    E-print Network

    Central Florida, University of

    (Salix caroliniana): Environmental effects and cost-efficient management. Postgraduate Research Assistant., Zhang, X., Ge, Q., and Tang, Y., 2013. Spatiotemporal analysis of ground-based woody plant leafing the non-point source pollution load of the typical small watershed in Miyun area. Journal of Irrigation

  16. Hibiscus rosa-sinensis cv. Christopher Howie (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    to Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Approved as to style and content by: Denis J. Phares (Chair of Committee) Kenneth D. Kihm (Member) Peter M. McIntyre (Member) Dennis L. O? Neal (Head... to thank him for his meaningful discussions and insights that helped me succeed in this research. I would like to thank Dr. Kenneth Kihm for being on my committee and for the various resources he provided for the success of this research. I would like...

  17. Gabrielle Katz, CV, Page 1 of 9 GABRIELLE L. KATZ

    E-print Network

    : Susan Beatty 1991 BA Geological Sciences, Brown University, Providence, Rhode Island Senior Thesis of Geography & Planning, Appalachian State University, Boone, NC 2010-present Associate Professor Department of Geography & Planning, Appalachian State University, Boone, NC Permanent tenure and promotion to Associate

  18. CV Marianne Espeland Marianne Espeland, PhD

    E-print Network

    Espeland, Marianne

    D) in systematic zoology, Stockholm University/ Swedish Museum of Natural History, Stockholm, Sweden. Thesis title zoology, Stockholm University. Thesis:"New Caledonian caddisflies: Taxonomy, phylogenetics and what rocks) - Systematics and diversification of Riodinidae 2011-2014 Postdoctoral fellow, Museum of Comparative Zoology

  19. Hibiscus rosa-sinensis cv. California Gold (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    stream_source_info Texas Gold Rush.pdf.txt stream_content_type text/plain stream_size 10484 Content-Encoding ISO-8859-1 stream_name Texas Gold Rush.pdf.txt Content-Type text/plain; charset=ISO-8859-1 tx H2O | pg. 6... Texas Gold Rush Story by Kathy Wythe A free-floating microscopic alga is increasingly appearing in some Texas lakes and rivers, releasing its deadly toxins and wiping out millions of fish. Texas scientists have taken on the assignment...

  20. DISCHER CV Mar. 2015 Page 1 of 26

    E-print Network

    Discher, Dennis

    matrices and amphiphilic assemblies are made and applied across topics, recognizing that most cell Plenary Award Lecture ­ Food, Pharm, Bioeng. Division (Nov 2009) ...among ~450 Seminars, Lectures, Invited

  1. Katherine Heal CV -page 1 A. CURRICULUM VITAE

    E-print Network

    -destroying methyl halide gases in situ in tropical forests; Identifying the processes by which plant growth promoting bacteria enhance phytoremediation of metal-contaminated soils; Demonstrating that schemes

  2. Jason Aughenbaugh: CV Page 1 JASON MATTHEW AUGHENBAUGH

    E-print Network

    the modeling and management of uncertainty in security, defense, energy, and industrial applications, including and several journal papers. In the future, I plan to draw from my industrial experience and improve ­ Aug. 2006 Graduate Independent Work Special Problems Course, Georgia Institute of Technology - Studied

  3. Hibiscus rosa-sinensis cv. Sylvia Goodman (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    OF CIRCADIAN CHANGES IN THE RETINAS OF MIGRATING AND NON-MIGRATING BLACKCAPS, Sylvia atricapilla A Senior Scholars Thesis by ROSS SHOCKLEY Submitted to the Office of Undergraduate Research Texas A&M University in partial fulfillment... of the requirements for the designation as UNDERGRADUATE RESEARCH SCHOLAR April 2006 Major: Biology COMPARISON OF CIRCADIAN CHANGES IN THE RETINAS OF MIGRATING AND NON-MIGRATING BLACKCAPS, Sylvia atricapilla A Senior Scholars...

  4. Bergeon Burns CV 1 CHRISTINE M. BERGEON BURNS

    E-print Network

    Ketterson, Ellen D.

    / Principal Instructor, Indiana U. Animal Behavior. 2011 Assistant Instructor, Indiana U. Epigenetics, Department of Biology Program in Evolution, Ecology and Behavior; Concentration: Behavior and Physiology Minor: Animal Behavior (Area Certificate) Advisors: Ellen D. Ketterson and Gregory E. Demas B.S. 2001

  5. E. M. Sigel CV -1 Erin M. Sigel

    E-print Network

    . American Fern Journal 104.1: 22­23. 2014 Li, F.W., J.C. Villarreal, S. Kelly, C.J. Rothfels, M. Melkonian, T. Chen, S. Weststrand, P. Thomas, E. Carpenter, Y. Zhang, Z. Tian, L. Chen, Z. Yan, Y. Zhu, X. Sun. Brittonia, DOI 10.1007/s12228-014-9332-6. 2013 Rothfels, C. J., A. Larsson, F. W. Li, E. M.

  6. Cuervo, Andrs M. -CV ANDRS M. CUERVO, Ph.D.

    E-print Network

    Cuervo, Andrés

    Station, American Museum of Natural History, Portal, A, AND HONORS: 2013. Outstanding Graduate Student Award. Museum of Natural Science acuervom at tulane dot edu New Orleans, Louisiana, 70118 http://www.museum

  7. Hibiscus rosa-sinensis cv. Orchid White (Cultivated) 

    E-print Network

    Beverly Thompson

    2011-08-10

    Spiranthes Parksii Correll is an endangered orchid of Texas. Populations of S. parksii are threatened by habitat loss and degradation resulting from human population growth and attendant development and resource extraction. Conservation easements...

  8. HARVARDgazette15-28 APRIL 2010 VOL. CV NO. 14

    E-print Network

    Wolfe, Patrick J.

    into the history of early medieval Britain through the lens of material culture. http://hvd.gs/42890 BILL GATES TO SPEAK AT SANDERS Microsoft founder and chairman Bill Gates will visit Harvard April 21 and examine

  9. Chemistry and Petrology of Chondrules from the Mokoia CV Chondrite

    NASA Technical Reports Server (NTRS)

    Jones, R. H.; Schilk, A. J.

    2000-01-01

    Bulk chemical compositions of 94 chondrules from Mokoia have been obtained by INAA. Elemental abundances are correlated with petrology. Chemical and petrological properties are very similar to chondrules from Allende and do not show strong alteration signatures.

  10. HARVARDgazette29 April-12 May 2010 VOL. CV NO. 15

    E-print Network

    Wolfe, Patrick J.

    , the Harvard Gazette has re- cently launched a mobile-friendly Web site. To dive deep into the Gazette new Rollins Senior Multimedia Producer Max Daniels Web Specialist Jennifer Hodge Web Content Producer Christopher Traganos Web Developer Distribution and Subscriptions: 617.495.4743 Delivered free to faculty

  11. HARVARDgazette29 OCT.-11 NOV. 2009 VOL. CV NO. 5

    E-print Network

    Wolfe, Patrick J.

    | Harvard Staff Photographers TO TELL THE TRUTH The rationale behind systemic torture is that pain will make the guilty confess, but a new study by researchers at Harvard University finds that the pain of torture can

  12. CV and List of Publications Emmanouil C. Zacharenakis

    E-print Network

    Stavroulakis, Georgios E.

    (HellenicSocietyofEarthquakeEngineering)http://www.eltam.gr/ BOOK PUBLICATIONS 1. Soil Mechanics and Foundation Engineering (in Greek). 1st ed. 1976, 2nd ed. 1978. 1st National Congress on Computational Mechanics, Athens, 3-4 Sep. 1992 , Vol. 1, pp 460-467. 2. G.E. Stavroulakis: "New trends in optimal structural control". In: "Selected Topics in Structronic and Mechatronic

  13. Daniel E. Lieberman CV, pg 1 CURRICULUM VITAE

    E-print Network

    Lieberman, Daniel E.

    Hominid 1032255. A new history and geography of human genes informed by ancient DNA (collaborator; PI Evolutionary Biology Harvard University 11 Divinity Avenue Cambridge, MA 02138 Phone: (617) 495-5479 Email, Harvard University 1987 M. Phil., Biological Anthropology, Cambridge University 1986 A.B., Summa cum Laude

  14. Spectroscopic Classification of ASASSN-15tx as a CV

    NASA Astrophysics Data System (ADS)

    Bersier, D.; Copperwheat, C.

    2015-12-01

    We report a spectroscopic observation of ASASSN-15tx (ATel #8400) obtained with the 2m robotic Liverpool Telescope (+SPRAT low-resolution spectrograph) on 2015 Dec 11.9078 UT. The spectrum, covering the 400-800 nm range, is blue and nearly featureless.

  15. Naomi Ellemers CV (20-11-2014) 1 Curriculum Vitae

    E-print Network

    .socialsciences.leiden.edu/psychology/organisation/so/staff/ellemers.html Education 1974-1980 Praedinius Gymnasium, Groningen, the Netherlands 1980-1981 University of California management strategies: The influence of socio-structural variables on strategies of individual mobility

  16. Last updated: Mar 31, 2014 CURRICULUM VITAE

    E-print Network

    : Division of Chemistry and Material Science RAS program, Russian Basic Research Foundation (research), INTAS, NATO.CLG As the participant: Russian Basic Research Foundation (research, practically-oriented research, international, infrastructure), INTAS, "Russian Universities" Teaching Courses · Physical Methods in Chemistry

  17. Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants a two-stage transformation protocol combining procedures of Webb and Wenzler...

  18. Effect of gamma irradiation on quality of kiwifruit ( Actinidia deliciosa var. deliciosa cv. Hayward)

    NASA Astrophysics Data System (ADS)

    kim, Kyoung-Hee; Yook, Hong-Sun

    2009-06-01

    Ionizing radiation is able to inactivate the three pathogens of Botrytis cinerea, Diaporthe actinidiae, and Botryosphaeria dothidea in kiwifruit. Irradiated kiwifruits appeared softer compared to non-irradiated kiwifruits. The color and organic acid content of kiwifruits were minimally affected by the irradiation. Irradiated fruits showed a decrease in the total soluble solids content with increasing irradiation dose. Irradiation of kiwifruit up to 3 kGy had negative effects on vitamin C content and antioxidant activity, but it contributed to improving sensory quality.

  19. Curriculum Vitae Esther Hagenlocher Prior to joining teaching in 2004 CV 1 | 13

    E-print Network

    -House", Langkawi Island Malaysia. Project architect for the concept design and color concept of a 3,500ft 2 Zirkus, Berlin-Friedrichstraße, Germany. Consultant for the design and color concept of a 2.050ft 2 and Design Stuttgart, Germany Dipl. Ing., Architecture and Design 1994 1988-1990 Major: Interior Architecture

  20. Chemical composition and physicochemical properties of green banana (Musa acuminata x balbisiana Colla cv. Awak) flour.

    PubMed

    Haslinda, W H; Cheng, L H; Chong, L C; Noor Aziah, A A

    2009-01-01

    Flour was prepared from peeled and unpeeled banana Awak ABB. Samples prepared were subjected to analysis for determination of chemical composition, mineral, dietary fibre, starch and total phenolics content, antioxidant activity and pasting properties. In general, flour prepared from unpeeled banana was found to show enhanced nutrition values with higher contents of mineral, dietary fibre and total phenolics. Hence, flour fortified with peel showed relatively higher antioxidant activity. On the other hand, better pasting properties were shown when banana flour was blended with peel. It was found that a relatively lower pasting temperature, peak viscosity, breakdown, final viscosity and setback were evident in a sample blended with peel. PMID:19449278

  1. Ashburn-Nardo CV March 24, 2015 1 Leslie Ashburn-Nardo

    E-print Network

    -2003 FELLOWSHIPS, HONORS, AND AWARDS 2015 Invited Mentor, Diversity and Climate Committee Reception, Society years 2014 Honorable Mention, Outstanding Teaching and Mentoring Award, Society for the Psychological Study of Social Issues 2014 Fellow, Society for the Psychological Study of Social Issues #12;Ashburn

  2. CV Dr. Oliver Neubauer 04/08/2014 CURRICULUM VITAE|DR. OLIVER NEUBAUER

    E-print Network

    -1090 Vienna, Austria Telephone number: +43 1 4277 ­ 54941 E-mail: oliver.neubauer@univie.ac.at Web of molecular-biological data CAREER HISTORY 2013/14: Guest Professor, Dep. of Nutritional Sciences, Univ Research Centre, Griffith Univ., Australia 2010­2011: University Assistant, Dep. of Nutritional Sciences

  3. Processing effects on the composition of sea buckthorn juice from Hippophae rhamnoides L. Cv. Indian Summer.

    PubMed

    Beveridge, T; Harrison, J E; Drover, J

    2002-01-01

    Sea buckthorn juice is one product that can be derived from the sea buckthorn berry, a new alternative crop for the Canadian western provinces. Fresh pressed juice separates into three phases when allowed to stand overnight in the refrigerator: an upper cream phase, juice in the middle portion, and a sediment at the bottom. Enzymatic hydrolysis with commercial, broad spectrum carbohydrate hydrolyzing enzyme preparations reduced the juice viscosity, assisted juice separation, and provided an opalescent juice. Soluble solids averaged 10.2 degrees Brix, pH averaged 3.13, ascorbic acid averaged 174.2 mg/100 mL, and titratable acidity averaged 1.97% as malic acid all determined on centrifuged (10 000 rpm, 15 min) juice. Soluble sugars included glucose, fructose, and an unidentified component that was not sucrose or other common soluble monomeric or dimeric sugar. Quinic acid was quantitatively most important, while malic was next, and oxalic, citric, and tartaric acids were minor components. Washing berries by dipping reduced soluble solids (degrees Brix) in juice suggesting uptake of wash water. PMID:11754553

  4. Mineral chemistry and origin of spinel-rich inclusions in the Allende CV3 chondrite

    NASA Technical Reports Server (NTRS)

    Kornacki, A. S.; Wood, J. A.

    1985-01-01

    The present electron probe microanalysis of 20 spinel-rich inclusions in Allende indicates that the mineralogy of spinel-rich Allende inclusions is similar to that of fine grained Ca, Al-rich inclusions (CAIs) in ALH-77003 and rims on coarse grained CAIs in Allende. The mineralogy, mineral chemistry, and bulk chemistry of the inclusions indicate that they are not primitive aggregates of crystalline nebular condensates, but rather aggregates of minute concentric objects that are presently interpreted to be a fractionated distillation residue that lost Ca, Si-rich partial melt. It is suggested that interstellar dust aggregates were melted or distilled into Allende inclusions by aerodynamic drag heating, in regions of the nebula where the dust/gas ratio was so enhanced that the local oxygen fugacity was raised by several order of magnitude and liquids became thermodynamically stable.

  5. Economics of the Environment R. Quentin Grafton and John C.V. Pezzey

    E-print Network

    Pezzey, Jack

    alternatives. For a Palaeolithic hunter-gatherer the choice could have been between hunting for a deer, fishing a good (such as bread) or service (such as a haircut) from a shop, and life choices such as what job on how many desirable things in life we can have, because of limits to our time, our current income, our

  6. Effect of gamma radiation on the ripening and levels of bioactive amines in bananas cv. Prata

    NASA Astrophysics Data System (ADS)

    Gloria, Maria Beatriz A.; Adão, Regina C.

    2013-06-01

    Green Prata bananas at the full three-quarter stage were exposed to gamma radiation at doses of 0.0 (control), 1.0, 1.5 and 2.0 kGy and stored at 16±1 °C and 85% relative humidity. Samples were collected periodically and analyzed for peel color, pulp-to-peel ratio and levels of starch, soluble sugars and bioactive amines. Degradation of starch and formation of fructose and glucose followed first- and zero-order kinetics, respectively. Higher irradiation doses caused increased inhibitory effect on starch degradation and glucose formation. However, doses of 1.5 and 2.0 kGy caused browning of the peel, making the fruit unacceptable. Irradiation at 1.0 kGy was the most promising dose: it did not affect peel color, the pulp-to-peel ratio or the levels of the amines spermidine, serotonin and putrescine. However, it slowed down starch degradation and the formation and accumulation of fructose and glucose, delaying the ripening of the fruit for 7 days.

  7. Leaf protein from ammonia-treated dwarf elephant grass (Pennisetum purpureum Schum cv. Mott).

    PubMed

    Urribarrí, Lauris; Ferrer, Alexis; Colina, Alejandro

    2005-01-01

    Proteins can be an excellent byproduct of the biorefining of lignocellulosic materials. In this work, extraction conditions for the white leaf proteins (cytoplasmic) of ammonia-treated dwarf elephant grass were established to obtain a protein juice suitable for the production of leaf protein concentrates. A calcium hydroxide solution was used as extracting agent, at several solid-liquid ratios, pHs, temperatures, and times. Extractions were carried out in Erlenmeyer flasks containing 5 g (dry basis) of forage with constant agitation (100 rpm). The soluble protein content was determined by the Lowry method. Optimal extraction conditions for the ammonia-treated forage were 12.60 pH, 1:10 solid-liquid ratio, 90 degrees C, and 30 min extraction time, resulting in 52.65% extraction yield. The ammonia treatment significantly increased (p<0.05) the release of proteins from the fibrous matrix, facilitating their extraction. PMID:15920275

  8. Stereoselective dissipation of epoxiconazole in grape (Vitis vinifera cv. Kyoho) and soil under field conditions.

    PubMed

    Liang, Hongwu; Qiu, Jing; Li, Li; Li, Wei; Zhou, Zhiqiang; Liu, Fengmao; Qiu, Lihong

    2012-05-01

    Stereoselective dissipation of epoxiconazole had been studied in grape and soil during plant growing under field conditions in this paper. A sensitive and rapid chiral method was developed and validated for the determination of epoxiconazole stereoisomers in grape and soil based on liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS). Phenomenex Lux Cellulose-1 column was used for enantioseparation with a mixture of acetonitrile/water (90/10, v/v) as mobile phase at flow rate of 0.3 mL min(-1). Fortified recoveries in grape and soil samples ranged from 76.0% to 91.9% and relative standard deviations were less than 11.4% with fortified levels of 0.025-1.0 mg kg(-1). The limits of detection and quantification were 0.005 mg kg(-1) and 0.025 mg kg(-1), respectively, with linear calibration curves extending up to 5.0 mg kg(-1). The field experimental results showed that dissipations of epoxiconazole stereoisomers in grape followed first-order kinetics (R(2)>0.92) and stereoselectivity occurred in 2 h after spraying. The (-)-stereoisomer with half-life of 9.3 d degraded faster than (+)-stereoisomer with that of 13.2 d, and resulted in relative enrichment of (+)-stereoisomer. However, the stereoisomeric dissipations in soil were triphasic ("increase-decrease-steady") with lower dissipation rates, and also occurred with preferential degradation of (-)-stereoisomer under field condition. The results for stereoselective dissipations can be applied for food and environmental assessments of chiral pesticides. PMID:22414382

  9. Page 1 of 8 Academic CV, MCWhitton Last revised 2012-01-02

    E-print Network

    Pollefeys, Marc

    .S. Guidance and Personnel Services (1974) North Carolina State University, Raleigh, NC Thesis Title: Same-Sex and Cross-Sex Reliability and Concurrent Validity of the Strong-Campbell Interest Inventory B. A. Religion to Physical Therapy - Improving scenario/rehabilitation session generation tools for physical therapists - How

  10. Short CV -Roland Grafstrm 2013-07-01 Full Name: Lars Roland Charles Grafstrm

    E-print Network

    Linke, Heiner

    of 12 post docs, as well as leadership of international research organizations and arrangements.g., biological effects and metabolism of drugs, safety evaluation of environmental chemicals and nanomaterials

  11. QTLs associated with resistance in soybean PI567516C to synthetic nematode population infecting cv. Hartwig

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Worldwide, soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most destructive pathogen of soybean [Glycine max (L.) Merr.]. Crop losses are primarily mitigated by the use of resistant cultivars. Nematode populations are variable and have adapted to reproduce on resistant cultivars ov...

  12. Interpreting Data from Smoothed Particle Hydrodynamics Simulations of Accretion Disks in CV Systems

    NASA Astrophysics Data System (ADS)

    Guasch, Mark H.; Montgomery, M. M.

    2009-05-01

    We compare smoothed particle hydrodynamics (SPH) simulations of artificially tilted and non-tilted, quasi-static accretion disks in Cataclysmic Variable systems. The accretion disks are simulated in 3D with 100,000 particles to show resolution in the z-direction. For these simulations, we assume a secondary-to-primary mass ratio q=0.4 and low mass transfer rates. Comparing the densities, internal energy emissions, and velocities within each disk and between disks tilted twenty degrees and non-tilted disks, we seek to identify significant features and the source to negative superhumps. We present face-on and edge-on accretion disks. We would like to acknowledge grants from the AAS small research grant and the UCF/UF Space Research Initiative.

  13. The role of the SW Sextantis stars in the picture of CV evolution

    NASA Astrophysics Data System (ADS)

    Rodríguez-Gil, P.

    2005-08-01

    The SW Sextantis stars are nova-like cataclysmic variables (CVs) which exhibit intricate behaviour which is still a matter of debate. For years the common belief has been that these systems were the "outlaws" of the CVs, mainly because of the lack of an overall understanding. We are now realizing that a large percentage (˜ 30%) of all nova-likes in the ˜ 3-4 hour period range are SW Sex stars. The study of the dominance of this class just above the period gap will provide clues on the evolution of CVs. Here we present the discovery of 7 new SW Sex stars from the CVs found in the Hamburg Quasar Survey.

  14. New spectroscopic and photometric observations of CV J0644+3344

    NASA Astrophysics Data System (ADS)

    Hernández Santisteban, J. V.

    We report photometric and simultaneous spectroscopic observations of the cataclysmic variable J0644+3344 and present calibrated Doppler tomography results of the object. We have calibrated the spectra for slit losses using simultaneous photometry. This has been used to construct reliable Doppler images in the Halpha , Hbeta and He II 4686 Å@ emission lines. We have also performed a radial velocity analysis using these emission lines to derive the primary semi-amplitude and used a cophasing method to determine the secondary K_2 best value. We have improved the ephemeris of the object based on the published and our new photometric eclipse timings to obtain HJD=254474.7927+0.26937446E. We derived a K_1=123.2±6.6 {km s}-1 and K_2=205.4±6.7. Assuming a i>76, the orbital parameters are M_1=0.91±0.04 M?, M_2=0.83±0.04 M? and a=2.10±0.03 R?. Based on the Doppler tomography we conclude that J0644+3344 is a nova-like SW Sex type system.

  15. Page 1 of 7 Fleischman CV September 2014 Forrest D. Fleischman

    E-print Network

    , August 2013 - Present Dartmouth College Environmental Studies Program: Postdoctoral Research Associate of the Commons, 8(2). http://www.thecommonsjournal.org/index.php/ijc/article/view/416 Fleischman, F. D., Loken. International Journal of the Commons, 8(2). http://www.thecommonsjournal.org/index.php/ijc/article/view/409

  16. Pawel Swietojanski CV, December 2014, page 1 of 3 PAWEL SWIETOJANSKI

    E-print Network

    Edinburgh, University of

    Diamond Laureate (XII edition) 2007 BSc in Computer Science at Higher State Vocational School in Tarnow and Communications Technology, Kyoto, Japan (March-April 2014) 2009 MSc in Computer Science at AGH University grade 5/5 · Study in theoretical computer science · Thesis in speech recognition rated at 5/5, AGH

  17. Bush C.V., page 1 of 9 Address: Department of Biology

    E-print Network

    Clayton, Dale H.

    /Field Support: Ornithology & Adv. Field Ornithology, University of Utah 2004: Guest Lecturer: Heredity, University of Utah 1998-03: Teaching Assistant, University of Utah Advanced Field Ornithology 2000, 2001 and 2003 Ornithology 1999, 2000 Plant Systematics 1999 Organismal Diversity, Form and Function 1998

  18. In vitro characterization of the Meq proteins of Marek's disease virus vaccine strain CV1988

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Gallid herpesvirus 2 (GaHV-2), commonly known as Marek’s disease virus serotype-1 (MDV-1), causes T cell lymphoma in chickens. Vaccines prepared from the CVI988/Rispens MDV-1 strain currently offers the best protection. Although, CVI988 is non-oncogenic, it codes for at least two forms of the MDV on...

  19. Inheritance of Resistance in Soybean PI 567516C to LY1 Nematode Population Infecting cv. Hartwig

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Worldwide, cyst nematode (SCN: Heterodera glycines Ichinohe) is the most destructive pathogen on soybean [Glycine max (L.) Merr.]. In the USA yield losses in 2001 were estimated to be nearly sixty million dollars. Crop losses are primarily reduced by the use of resistant cultivars. Nematode populat...

  20. Comparative Studies of Antioxidant Activities and Nutritional Constituents of Persimmon Juice (Diospyros kaki L. cv. Gapjubaekmok)

    PubMed Central

    Lee, Jin Hwan; Lee, Yong Bok; Seo, Woo Duck; Kang, Su Tae; Lim, Jong Woo; Cho, Kye Man

    2012-01-01

    The objectives of this research were to evaluate antioxidant activities and nutritional components, including phenolic acid, catechin, organic acid, sugar, and amino acid, of persimmon juice from persimmons grown in different regions around Korea. Persimmon (Diospyros kaki) exhibits potent antioxidant effects in DPPH, ABTS, reducing power, and FRAP methods of analysis. The levels of nutritional constituents showed significant differences among all the samples. In particular, tartaric acid, glucose, gallic acid, epicatechin gallate and aspartic acid were observed to be the predominant component for each of their general chemical groups, with total average contents of 1876.51 mg/kg, 62.69 g/kg, 12.73 mg/kg, 208.99 mg/kg, and 31.84 mg/100 g, respectively. Interestingly, persimmons from the Hadong region presented the highest sugar (130.60 g/kg), phenolic acid (42.27 mg/kg), and catechin (527.97 mg/kg) contents in comparison with other regional samples. Moreover, this location exhibited the greatest antioxidant activity with highest total phenolic (298.01 mg GAE/kg) and flavonoid (32.11 mg/kg RE) contents. Our results suggest that strong antioxidant activities of persimmons correlate with high phenolic acid and catechin contents, particularly gallic acid and epicatechin gallate. Additionally, these two compounds may be key factors when considering the useful ingredients of persimmon. PMID:24471076