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1

Levels of job satisfaction and role perceptions held by extension agents of INTA'S Regional Parana in Argentina  

E-print Network

Extension Agency l2 i I 3 j~ FIGURE 1. ' Services of the National Institute of Agricultural Technology (INTA). INTA REGIONAL PARANA ~ l. a Par ~ Federal Cn, Vari Province of Concordia ~ ~ Bovrd ENTRE RIOS ~ Villaguiiy e Parand ~ Crespo...

Blaha, Francisco Jose

1983-01-01

2

Monitoring of biologically effective UV irradiance at El Arenosillo (INTA), Andalucía, in Spain  

Microsoft Academic Search

Biological UV (ultraviolet) dosimetry was applied using the biofilm-technique (DLR patent) to determine the UV levels weighted of biologically weighted UV radiation at the INTA Sounding Station of El Arenosillo at Huelva, Spain (37° 06?N, 6° 44?W, 50 m.a.s.l.= above sea level) on 2 days in 1977 (April 1, and May 5). Exposure periods were calculated for clear sky days

R. de la Torre; G. Horneck; P. Rettberg; E. Luccini; J. M. Vilaplana; M. Gil

2000-01-01

3

Freiburg RNA Tools: a web server integrating IntaRNA, ExpaRNA and LocARNA  

E-print Network

The Freiburg RNA tools web server integrates three tools for the advanced analysis of RNA in a common web-based user interface. The tools IntaRNA, ExpaRNA and LocARNA support the prediction of RNA–RNA interaction, exact ...

Smith, Cameron

4

Why Include Bibliometric Analysis in the Activities of a Library Specialized in Astronomy? — Notes From the Libraries of INTA  

NASA Astrophysics Data System (ADS)

We describe the different possibilities that several bibliometric studies offer the National Institute of Aerospace Technology (INTA) and the Center for Astrobiology (CAB) libraries. The main object in this paper is to show how the results of these investigations are useful for various matters, such as preservation options, exploitation and diffusion of these results, and facilitating internal information management.

Alonso-Valdivielso, M. Á.; Antonio, E. G.

2010-10-01

5

End-to-end validation process for the INTA-Nanosat-1B Attitude Control System  

NASA Astrophysics Data System (ADS)

This paper describes the end-to-end validation process for the Attitude Control Subsystem (ACS) of the satellite INTA-NanoSat-1B (NS-1B). This satellite was launched on July 2009 and it has been fully operative since then. The development of its ACS modules required an exhaustive integration and a system-level validation program. Some of the tests were centred on the validation of the drivers of sensors and actuators and were carried out over the flying model of the satellite. Others, more complex, constituted end-to-end tests where the concurrency of modules, the real-time control requirements and even the well-formedness of the telemetry data were verified. This work presents an incremental and highly automatised way for performing the ACS validation program based on two development suites and an end-to-end validation environment. The validation environment combines a Flat Satellite (FlatSat) configuration and a real-time emulator working in closed-loop. The FlatSat is built using the NS-1B Qualification Model (QM) hardware and it can run a complete version of the on-board software with the ACS modules fully integrated. The real-time emulator, running on an industrial PC, samples the actuation signals and emulates the sensors signals to close the control loop with the FlatSat. This validation environment constitutes a low-cost alternative to the classical three axes tilt table, with the advantage of being easily configured for working under specific orbit conditions, in accordance with any of the selected tests. The approach has been successfully applied to the NS-1B in order to verify different ACS modes under multiple orbit scenarios, providing an exhaustive coverage and reducing the risk of eventual errors during the satellite's lifetime. The strategy was applied also during the validation of the maintenance and reconfiguration procedures required once the satellite was launched. This paper describes in detail the complete ACS validation process that was performed and it shows the most relevant errors detected and fixed during testing. Finally it summarises some of the most significant conclusions.

Polo, Óscar R.; Esteban, Segundo; Cercos, Lorenzo; Parra, Pablo; Angulo, Manuel

2014-01-01

6

Characterization of IntA, a Bidirectional Site-Specific Recombinase Required for Conjugative Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42  

PubMed Central

Site-specific recombination occurs at short specific sequences, mediated by the cognate recombinases. IntA is a recombinase from Rhizobium etli CFN42 and belongs to the tyrosine recombinase family. It allows cointegration of plasmid p42a and the symbiotic plasmid via site-specific recombination between attachment regions (attA and attD) located in each replicon. Cointegration is needed for conjugative transfer of the symbiotic plasmid. To characterize this system, two plasmids harboring the corresponding attachment sites and intA were constructed. Introduction of these plasmids into R. etli revealed IntA-dependent recombination events occurring at high frequency. Interestingly, IntA promotes not only integration, but also excision events, albeit at a lower frequency. Thus, R. etli IntA appears to be a bidirectional recombinase. IntA was purified and used to set up electrophoretic mobility shift assays with linear fragments containing attA and attD. IntA-dependent retarded complexes were observed only with fragments containing either attA or attD. Specific retarded complexes, as well as normal in vivo recombination abilities, were seen even in derivatives harboring only a minimal attachment region (comprising the 5-bp central region flanked by 9- to 11-bp inverted repeats). DNase I-footprinting assays with IntA revealed specific protection of these zones. Mutations that disrupt the integrity of the 9- to 11-bp inverted repeats abolish both specific binding and recombination ability, while mutations in the 5-bp central region severely reduce both binding and recombination. These results show that IntA is a bidirectional recombinase that binds to att regions without requiring neighboring sequences as enhancers of recombination. PMID:23935046

Hernández-Tamayo, Rogelio; Sohlenkamp, Christian; Puente, José Luis; Brom, Susana

2013-01-01

7

CV Surgery Online  

NSDL National Science Digital Library

Stanford University's HighWire Press has announced the online publication of several journals. CV Surgery Online offers "a fully searchable online compilation of articles pertinent to the cardiothoracic and vascular surgeon from the 5 American Heart Association Journals: Circulation, Circulation Research, Hypertension, Stroke, and Arteriosclerosis, Thrombosis, and Vascular Surgery." Published by the American Heart Association in conjunction with HighWire Press, the free trial period for CV Surgery Online ended December 31, 2000.

1999-01-01

8

Gilbert CV 1 BENJAMIN GILBERT  

E-print Network

. Plant invasions and the niche. Journal of Ecology 97:609-615. Srivastava, D.S., M.K. Trzcinski, BGilbert ­ CV 1 BENJAMIN GILBERT Department of Ecology, Evolution and Marine Sciences Marine. Ecology 87:1281-1288. Gilbert, B., and M.J. Lechowicz. 2005. Invasibility and abiotic gradients

Gilbert, Benjamin

9

Biradavolu_CV October 2011  

E-print Network

." Health Communication. 13(2): 205-226. #12;Biradavolu_CV 3 1999 Lipkus, Isaac, Yancey Crawford, Kathryn for communicating colorectal cancer risk." Journal of Health Communication. 4:311- 324. SELECTED TALKS on Health, Risk and Society, American University 2010 Research Scholar, Duke Global Health Institute, Duke

Lansky, Joshua

10

CV Johannes Bhm Curriculum Vitae  

E-print Network

CV Johannes Böhm 1/3 Curriculum Vitae Name: Univ. Prof. Dr. Johannes Böhm Chair of Advanced Geodesy Nationality: Austria Address: Department of Geodesy and Geoinformation E120/4 Vienna University of Technology Baseline Interferometry Atmospheric Effects in Space Geodesy Reference Frames Employment Record 07

Schuh, Harald

11

CV  

E-print Network

Classes in classical mechanics, electrodynamics, quantum ... Finite-size Scaling for Atomic and Non-equilibrium Systems”. (joint project with ... Title: “Topological charges and the geometry of momentum space”. Oct 13 .... tails and Dirac points.

2014-10-29

12

CV  

E-print Network

Aug 14, 2014 ... Analysis of Biological Networks, A. Jayaraman and J. Hahn, Eds. Boston/London: Artech House, ... Bazil, J.N., Buzzard, G.T., Rundell, A.E., Modeling mitochondrial bioenergetics with integrated ... Classification using Digital Compressive Detection, Analytica Chimica Acta, .... Advanced Services Committee.

2014-08-14

13

Koray Aydin -CV 1 KORAY AYDIN  

E-print Network

Koray Aydin - CV 1 KORAY AYDIN EDUCATION Ph.D. Physics, Bilkent University 2008 B.S. Physics. Pryce, K. Aydin, R. Briggs, H. A Atwater, Tunable compliant optical metamaterials structures, US Patent Aydin - CV 3 1) K. Aydin, V. E. Ferry, R. M. Briggs, and H. A. Atwater, "Broadband, polarization

Aydin, Koray

14

mRNA vaccine CV9103 and CV9104 for the treatment of prostate cancer.  

PubMed

Among currently available vaccine strategies for cancer, nucleotide-based vaccination is an appealing treatment modality. Curevacs' mRNA containing vaccines (RNActive®) combine the beneficial properties of sufficient antigen-expression, autologous immune-stimulation and a high flexibility with respect to production and application. CV9103 and CV9104 are novel RNActive®-derived anticancer vaccines for the treatment of patients with prostate cancer. After successful phase I/II studies with documentation of good tolerability and favorable immune-activation of CV9103, the vaccine CV9104 is currently undergoing clinical testing in specific clinical settings such as castration resistant prostate cancer and as a neoadjuvant agent in men with high risk prostate cancer prior to surgery. This review discusses the available preclinical and clinical data on the anticancer vaccination treatment with RNActive®-derived anticancer-vaccines CV9103 and CV9104. PMID:24999635

Rausch, Steffen; Schwentner, Christian; Stenzl, Arnulf; Bedke, Jens

2014-07-01

15

mRNA vaccine CV9103 and CV9104 for the treatment of prostate cancer.  

PubMed

Among currently available vaccine strategies for cancer, nucleotide-based vaccination is an appealing treatment modality. Curevacs' mRNA containing vaccines (RNActive®) combine the beneficial properties of sufficient antigen-expression, autologous immune-stimulation and a high flexibility with respect to production and application. CV9103 and CV9104 are novel RNActive®-derived anticancer vaccines for the treatment of patients with prostate cancer. After successful phase I/II studies with documentation of good tolerability and favorable immune-activation of CV9103, the vaccine CV9104 is currently undergoing clinical testing in specific clinical settings such as castration resistant prostate cancer and as a neoadjuvant agent in men with high risk prostate cancer prior to surgery. This review discusses the available preclinical and clinical data on the anticancer vaccination treatment with RNActive®-derived anticancer-vaccines CV9103 and CV9104. PMID:25483661

Rausch, Steffen; Schwentner, Christian; Stenzl, Arnulf; Bedke, Jens

2014-07-01

16

[Application of Tiantu (CV 22) in emergency].  

PubMed

The operation and application of Tiantu (CV 22) in emergency are discussed in the paper. The stimulating methods at Tiantu (CV 22) are acupuncture and pressing technique. The correct insertion of needle and proper depth and direction of insertion are required during acupuncture. The pressing technique stress the pressing strength and pressing time. Acupuncture or pressing technique is suitable for the emergent measurement of asthma, asthmatic breathing, coma, blockage of phlegm, hiccup, sore throat, etc. It is indicated that Tiantu (CV 22) is the key point in the emergency and phlegm resolving. Based on the characteristics of the point as promoting qi circulation, reducing the reversed qi and resolving phlegm, in light of the proper points combination by different syndromes and in terms of the correct and safe stimulating methods, Tiantu (CV 22) can achieve the immediate therapeutic effects in the emergent situations. PMID:23967641

Zhu, Xian-Min; Huo, Shang-Fei; Lu, Lu; Wang, Xin-Zhi

2013-06-01

17

Year Action IB # Jig HAL CP HAL CV Trawl CP Trawl CV  

E-print Network

CV Rockfish Program Cooperati ves TOTAL TAC ABC OFL for entire GOA 2012 42,705 56,940 104 = Overfishing Level CV = Catcher Vessel CP = Catcher Processor Rockfish Program Cooperatives this allocation for entire GOA 2012 21,024 28,032 104,000 Initial Allocation 315 7,869 4,100 290 497 7,952 Reallocation

18

CV (Mercado). February 2005 NORMAN MERCADO SILVA  

E-print Network

CV (Mercado). February 2005 NORMAN MERCADO SILVA Center for Limnology University of Wisconsin - Madison; 2001. PhD: Zoology and Limnology, Department of Zoology, University of Wisconsin ­ Madison, 2005 - Postdoctoral Associate. Center for Limnology (University of Wisconsin - Madison) 2004 - 05 Research Assistant

Mercado-Silva, Norman

19

Koray Aydin -CV 1 KORAY AYDIN  

E-print Network

Koray Aydin - CV 1 KORAY AYDIN EDUCATION Ph.D. Physics, Bilkent University 2008 B.S. Physics Handbook, Edited by Filippo Capalino, CRC Press (2009). PATENTS 1) I. Pryce, K. Aydin, R. Briggs, H. Briggs, and H. A. Atwater, "Broadband, polarization- independent resonant light absorption using

Aydin, Koray

20

Rachel E. Pepper CV 1 of 4 Rachel E. Pepper  

E-print Network

Rachel E. Pepper ­ CV ­ 1 of 4 Rachel E. Pepper University of California at Berkeley, 1005 Valley://spot.colorado.edu/~pepperr · e-mail: rachel.pepper@berkeley.edu EDUCATION Harvard University (Cambridge, MA) Ph.D., Physics University, 2005-2009 Graduate Researcher Advisor: Howard A. Stone #12;Rachel E. Pepper ­ CV ­ 2 of 4 Thesis

Koehl, Mimi

21

A New CV3 Chondrite Find  

NASA Astrophysics Data System (ADS)

A new meteorite find from West Texas, U.S.A., is a CV3 carbonaceous chondrite. The provisional name of Red Bluff has been proposed to the Nomenclature Committee by R. Farrell. Red Bluff consists of chondrules (56.4%), CAIs (8.3%), amoboid olivine aggregates (0.6%), mineral fragments (0.8%), and Ca-Al chondrules (0.4%) in a fine-grained, clay-rich matrix (33.1%). Chondrules in Red Bluff are spherical to irregular in shape, and from 0.25-3.5 mm diameter in thin section; the average diameter is 0.95 mm, with standard deviation of 0.6 mm (69 chondrules). Three of the chondrules are distinctly larger than the rest; without these three, the average diameter is 0.86 mm (s.d. 0.4 mm). The chondrules are almost all of type I (Fe-poor), as shown by cathodoluminescence and chemical analyses; most are also rich in opaques. Compositions of chondrule olivines average Fa1.9+-1.2 (s.d.); compositions of chondrule pyroxenes average Fs3.4+-3.3 (s.d.). Chondrule varieties include porphyritic olivine, microporphyritic olivine, granular olivine, macroporphyritic olivine, barred olivine, and rare extracentroradial pyroxene (0.25 mm diam.) [1,2]. Two calcium-aluminum chondrules were observed. The largest, 1.5 mm diam., contains spinel, plagioclase, and fassaite, and includes a circular spinel palisade [3]. The other Ca-Al chondrule is within a coarse-grained CAI, and could also be a well-developed spinel palisade [3]. Fine- and coarse-grained CAIs are present but have been studied little; most appear to be type B (melilite+pyroxene+plagioclase). Red Bluff's matrix is composed of fine-grained clay, with minor olivine, "limonite," troilite, and Fe metal. Alignment of grains and oxide-rich streaks in the matrix mark a planar fabric that wraps around chondrules and inclusions. Chondrules are commonly surrounded by shells of dark red alteration, darker than the bulk of matrix material. Red Bluff is weathered. It is stained red by oxidized iron minerals, which are most common as veinlets (after Fe metal or troilite?) and as rinds around Fe metal and troilite. The matrix clays may have formed during weathering of an olivine matrix similar to that of Allende. However, Fe metal and troilite remain common, both occluded within silicates and as discrete grains in the matrix. Cracks in Red Bluff are partially filled by minute, euhedral carbonate crystals. Red Bluff's exterior is coated with desert varnish (light-brown anisotropic film composed of many fine laminae); the varnish fills void space in one carbonate-rich veinlet. Classification Red Bluff's petrography, mineralogy, and mineral chemistry fall within the range of known CV3s [4]. Most characteristic are the proportion of matrix (33%) and the size of chondrules (avg ~1 mm), which are consistent with CV and inconsistent with known CI, CO, CM, CR, and CK chondrites. The proportions of CAIs, Ca-Al chondrules, and mineral fragments are also consistent with known CVs. The presence of type 1 and type 2 chondrules, and the variations in olivine compositions among type 1 chondrules implies that Red Bluff is of metamorphic grade 3. Compared to other CV3s, Red Bluff has rather little matrix and rather magnesian silicate minerals [4]. Red Bluff does not appear to be paired with known CV3s, and in fact none are known from West Texas [5]; the closest CV3 fall or find is Leoville (KS) [5]. This work was conducted under an NRC Fellowships to Treiman (senior) and DeHart (regular). We are grateful to R. Farrell for the meteorite sample, data on its source, and its proposed name. [1] Scott E. R. D. and Taylor G. J. (1983) Proc. 14 LPSC, B275. [2] Jones R. (1992) GCA 56, 467. [3] Wark D. A. and Lovering J. F. (1982) GCA 46, 2595. [4] McSween H. Y. Jr. (1977) GCA 71, 1777. [5] Graham et al. (1985) Catalog of Meteorites.

Treiman, A. H.; Dehart, J. M.

1992-07-01

22

CV-Muzar Using a Multiagent System for Group Formation  

NASA Astrophysics Data System (ADS)

The purpose of this paper is to present two agents’ societies responsible for group formation (sub-communities) in CV-Muzar (Augusto Ruschi Zoobotanical Museum Virtual Community of the University of Passo Fundo). The first is a static society that intends to investigate the groups in the CV-Muzar. The second is a dynamical society that will analyze the existing groups and look for participants that have common subjects in order to constitute a sub-community. The formation of sub-communities is a new module within the CV-Muzar that intends to bring the participants together according to two scopes: similarity of interests and knowledge complementarities.

de Marchi, Ana Carolina Bertoletti; Moraes, Márcia Cristina; Testa, Cristiane Durigon

23

ASAS-SN Discovery of a Likely CV  

NASA Astrophysics Data System (ADS)

During the ongoing All Sky Automated Survey for SuperNovae (ASAS-SN or "Assassin"), using data from the quadruple 14-cm "Brutus" telescope in Haleakala, Hawaii, we discovered a new transient source, likely a CV.

Davis, A. B.; Shappee, Bart; Shappee, B. J.; Will, D.; Simonian, G.; Stanek, K. Z.; Holoien, T. W.-S.; Kochanek, C. S.; Basu, U.; Gross, N.; Beacom, J. F.; Prieto, J. L.; Bersier, D.; Brimacombe, J.; Dong, Subo; Wozniak, P. R.; Szczygiel, D.; Pojmanski, G.

2014-12-01

24

Micropropagation of sour cherry ( Prunus cerasus L.) cv. Šumadinka  

Microsoft Academic Search

Sour cherry cv. Šumadinka (Prunus cerasus L.) is the leading Yugoslav cultivar for production orchards. A method of micropropagation has been developed for the purpose of growing ‘Šumadinka’ on its own roots and for rapid multiplication.

Radosav Cerovi?; Djurdina Ruži?

1987-01-01

25

R. MICHAEL YOUNG CV MAY 2014 R. Michael Young  

E-print Network

!1 R. MICHAEL YOUNG CV MAY 2014 R. Michael Young Professor Department of Computer Science Campus://liquidnarrative.csc.ncsu.edu/rmy/ ! BIOGRAPHIC SUMMARY ! Michael Young is a Professor of Computer Science at North Carolina State University

Young, R. Michael

26

Garca CV/p1 Dana M. Garca  

E-print Network

Foundation Graduate Fellow GRANTS #12;García CV/p2 Current 2003-2005 NSF (IBN 0235523) RUI: Pigment Granule (IBN 0077666) RUI: Pigment Granule Dispersion in Teleost Retinal Pigment Epithelium: Cholinergic

Aspbury, Andrea S. - Department of Biology, Texas State University

27

Comments on duck circovirus (DuCV) genotype definition.  

PubMed

A standardised methodology has been used to define genotypes based on pairwise sequence comparisons (PASC). PASC is a widely accepted method in virus taxonomy, which is based on the histogram of pairwised differences among sequences. Recently, Zhang et al. (2013) concluded that the average p-distance of duck circovirus (DuCV) between genotypes 1 and 2 was 0.170, and subtype distance thresholds were 0.032 in DuCV-1 and 0.018 in DuCV-2, respectively. However, there might be some concerns on the methodology application to define the genotype of DuCV. Taking into account the concerns mentioned above, our authors conducted the PASC analyses of 54 capsid gene (ORF2) and genomic sequences including all the sequences from Zhang et al. (2013). Our results confirmed the existence of two DuCV genotypes (1 and 2) and, we suggest that DuCV ORF2 and genomic distance genotype thresholds were 0.061 and 0.038, respectively. PMID:24418697

Wen, Huiqiang; Wu, Yongshu; Yang, Chao; Zhang, Xiaona; Lian, Chuanjiang; Chen, Hongyan; Han, Lingxia

2014-03-15

28

Micrografting of pistachio (Pistacia vera L. cv. Mateur)  

Microsoft Academic Search

A successful micrografting technique was developed for Pistacia vera. High levels of graft union were achieved when shoots from Stage II cultures of four-year-old P. vera. cv. Mateur were grafted onto in vitro-raised seedling rootstocks. Light and fluorescence microscopy investigations revealed that vascular continuity was established across grafts by three weeks.

A. Abousalim; S. H. Mantell

1992-01-01

29

Shane J. Macfarlan CV Shane J. Macfarlan, Ph.D.  

E-print Network

Shane J. Macfarlan CV 1 Shane J. Macfarlan, Ph.D. 14204 NE Salmon Creek Ave. Department and Research Interests Geographic Regions: Latin America, Caribbean, Dominica, and Baja California Sur, Mexico and Statistical Methods; History of Anthropological Theory; Philosophy of Science; Globalization & Contemporary

30

Rachel E. Pepper CV 1 of 5 Rachel E. Pepper  

E-print Network

Rachel E. Pepper ­ CV ­ 1 of 5 Rachel E. Pepper University of California Berkeley, 1005 Valley Life Sciences Bldg #3140, Berkeley CA 94720-3140 cell: 617-272-5914 · web: ib.berkeley.edu/labs/koehl/pepper/pepper.html · e-mail: rachel.pepper@berkeley.edu EDUCATION Harvard University (Cambridge, MA) Ph.D., Physics

Koehl, Mimi

31

Rachel E. Pepper CV 1 of 5 Rachel E. Pepper  

E-print Network

Rachel E. Pepper ­ CV ­ 1 of 5 Rachel E. Pepper University of California at Berkeley, 1005 Valley Life Sciences Bldg #3140, Berkeley CA 94720-3140 cell: 617-272-5914 · web: ib.berkeley.edu/labs/koehl/pepper/pepper.html · e-mail: rachel.pepper@berkeley.edu EDUCATION Harvard University (Cambridge, MA) Ph.D., Physics

Koehl, Mimi

32

Nematodes associated with pawpaw (cv. homestead) in Nsukka, Nigeria.  

PubMed

In field and greenhouse experiments, Meloidogyne javanica caused symptomatic damage to pawpaw (cv. Homestead). Other important nematodes to which pawpaw seemed a good host included Pratylenchus spp., Rotylenchus reniformis, Helioctylenchus spp., and Tylenchulus martini. Pawpaw appeared as a poor host to Scutellonema clatbricaudatum and Hoplolaimus pararobustus. Fusarium sp. was isolated from the plant roots. PMID:7342958

Ogbuji, R O

1981-01-01

33

CV Marianne Espeland Marianne Espeland, PhD  

E-print Network

CV Marianne Espeland Marianne Espeland, PhD Museum of Comparative Zoology, Room 401c, Harvard@fas.harvard.edu Current position Harvard University. Museum of Comparative Zoology and Department of Organismic and Evolutionary biology. Postdoctoral fellow Education Oct. 2010 Doctor of Philosophy (PhD) in systematic zoology

Espeland, Marianne

34

Individual Responsiveness to Information in CV Surveys : Commitment Matters  

Microsoft Academic Search

This paper enquires into the responsiveness of individuals to information in Contingent Valuation (CV). The impact of information is assessed using a sequential procedure in which individuals are successively presented with different levels of information. Two different types of information have been provided: scientific information about the good and information about the willingness to pay (WTP) values of the other

Olivier Chanel; Susan Cleary; Stéphane Luchini

2007-01-01

35

Ana M. Leon CV-2014 1 Revised: August 2014  

E-print Network

, 2010 Award, Women Making History: The Faculty Women Prominence Project, University of Central #12;AnaAna M. Leon CV-2014 1 Revised: August 2014 CURRICULUM VITAE Ana M. Leon, Ph.D, L.C.S.W. Professor Office Phone (407) 823-6195 Fax: (407) 823-5697 E-mail: Ana.Leon@ucf.edu EDUCATION: 1992 New York

Foroosh, Hassan

36

David Keith, Academic CV (Feb-13) Page 1 David Keith  

E-print Network

sensitivity to solar PV. Environmental impacts of energy technologies including the development of Canada #12;David Keith, Academic CV (Feb-13) Page 2 impact of large-scale wind power; and most recently of Calgary, Canada Research Chair in Energy and the Environment; Director and Professor, ISEEE Energy

37

Axtell, a new CV3 chondrite find from Texas  

Microsoft Academic Search

We describe a previously unreported meteorite found in Axtell, Texas, in 1943. Based on the mineralogical composition and texture of its matrix and the sizes and abundance of chondrules, we classify it as a CV3 carbonaceous chondrite. The dominant opaque phase in the chondrules is magnetite, and that in refractory inclusions is Ni-rich metal (awaruite). Axtell, therefore, belongs to the

S. B. Simon; L. Grossman; I. Casanova; S. Symes; P. Benoit; D. W. G. Sears; J. F. Wacker

1995-01-01

38

Computational simulation of CV combination preferences in babbling  

PubMed Central

There is a tendency for spoken consonant-vowel (CV) syllables, in babbling in particular, to show preferred combinations: labial consonants with central vowels, alveolars with front, and velars with back. This pattern was first described by MacNeilage and Davis, who found the evidence compatible with their “frame-then-content” (F/C) model. F/C postulates that CV syllables in babbling are produced with no control of the tongue (and therefore effectively random tongue positions) but systematic oscillation of the jaw. Articulatory Phonology (AP; Browman & Goldstein) predicts that CV preferences will depend on the degree of synergy of tongue movements for the C and V. We present computational modeling of both accounts using articulatory synthesis. Simulations found better correlations between patterns in babbling and the AP account than with the F/C model. These results indicate that the underlying assumptions of the F/C model are not supported and that the AP account provides a better and account with broader coverage by showing that articulatory synergies influence all CV syllables, not just the most common ones. PMID:24496111

Nam, Hosung; Goldstein, Louis M.; Giulivi, Sara; Levitt, Andrea G.; Whalen, D. H.

2013-01-01

39

Cuervo, Andrs M. -CV ANDRS M. CUERVO, Ph.D.  

E-print Network

Cuervo, Andrés M. - CV 1 ANDR�S M. CUERVO, Ph.D. Curriculum Vitae (version 15-advisor), Bryan C. Carstens, Michael Hellberg, Frederick H. Sheldon, and Alan Afton. M undergraduate biology student. Facultad de Ciencias Exactas y Naturales [College

Cuervo, Andrés

40

Effects of paclobutrazol on avocado (Persea americana Mill.) cv. ‘Fuerte’  

Microsoft Academic Search

Adato, I., 1990. Effects of paclobutrazol on avocado (Persea americana Mill. ) cv. 'Fuerte'. Scientia Hortic., 45:105-115. Mature avocado trees (Persea americana Mill. ) of cultivar 'Fuerte' were sprayed with Cultar ~, a commercial formulation containing 25% paclobutrazol (ICI, U.K.). Hand spraying of individual trees before or at the flowering stage resulted in an increase in the number of harvested

I. Adato

1990-01-01

41

Boost Your CV! Training, Volunteering, Careers and Further Study  

E-print Network

Boost Your CV! Training, Volunteering, Careers and Further Study Training There are many opportunities to receive valuable free training from the University and the Students' Union. LLR training such as NOW and MS Office training, Referencing and dissertation research tips, plus training in some web

Evans, Paul

42

Indirect Moxibustion (CV4 and CV8) Ameliorates Chronic Fatigue: A Randomized, Double-Blind, Controlled Study  

PubMed Central

Abstract Objectives The antifatigue effect of indirect moxibustion and its antioxidant properties were investigated. Subjects and design A randomized, double-blind, controlled clinical trial was performed with 44 patients who had idiopathic chronic fatigue. The subjects were treated with a placebo or moxibustion (indirect moxibustion on CV4 and CV8 3 times per week for 4 weeks), and their fatigue severity was monitored using a self-rating numeric scale (NRS) and a visual analog scale (VAS). Serum level of reactive oxygen species and malondialdehyde (MDA), total antioxidant capacity, the activity of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase and total glutathione content, were determined before initial moxibustion therapy and after the 12th moxibustion treatment. Results The moxibustion group had a significantly lower fatigue severity score compared to the control for both the NRS (p<0.05) and VAS scores (p<0.01). The level of serum MDA was significantly lower in the moxibustion group than in the placebo group (p<0.05), whereas glutathione reductase activity and total glutathione content increased significantly following moxibustion (p<0.05). Conclusions The results provide clinical evidence for an antifatigue effect of indirect moxibustion at CV4 and CV8 and suggest that the effect is due to the antioxidant properties of moxibustion. PMID:22757691

Kim, Hyeong Geug; Yoo, Sa Ra; Park, Hye Jung

2013-01-01

43

Study of MnO2 Coverage on Ta Capacitors with High CV Powders  

Microsoft Academic Search

The use of high CV tantalum powders (>100k CV\\/g) in solid tantalum capacitors (STCs) has become one of the main goals of Ta capacitors manufacturers. The systems developed on the last years allowed a coverage increase for products with high CV powders and reliability enhancement. However, an important gap still remains unexplored being nowadays the current opportunity. This will allow

D. Dias; R. Monteiro; C. Mota-Caetano; E. Fortunatoe Pimentel

44

Axtrell, a new CV3 chondrite find from Texas  

NASA Technical Reports Server (NTRS)

We describe a previously unreported meteorite found in Axtell, Texas, in 1943. Based on the mineralogical composition and texture of its matrix and the sizes and abundance of chondrules, we classify it as a CV3 carbonaceous chondrite. The dominant opaque phase in the chondrules is magnetite, and that in refractory inclusions is Ni-rich metal (awaruite). Axtell, therefore, belongs to the oxidized subgroup of CV3 chondrites, although unlike Allende it escaped strong sulfidation. The meteorite bears a strong textural resemblance to Allende, and its chondrule population and matrix appear to be quite similar to those of Allende, but its refractory inclusions, thermoluminescence properties, and cosmogenic Co-60 abundances are not. Our data are consistent with a terrestrial age for Axtell of approximately 100 years and a metamorphic grade slightly lower than that of Allende.

Simon, S. B.; Grossman, L.; Casanova, I.; Symes, S.; Benoit, P.; Sears, D. W. G.; Wacker, J. F.

1995-01-01

45

CV Carlos Chesevar Diciembre 2009 Pg. 1 Carlos Ivn Chesevar  

E-print Network

CV Carlos Chesñevar ­Diciembre 2009 Pág. 1 Carlos Iván Chesñevar Curriculum Vitae 1. Datos personales Apellido y nombres : Chesñevar, Carlos Iván Fecha de nacimiento : 20 de julio de 1969. Lugar de/Fax (Universidad) : +54 ­ 291 ­ 459 5135 extensión 2603 / 459 5136 Dirección laboral : Departamento de Cs. e Ing

Maguitman, Ana Gabriela

46

[Total parenteral nutrition and the usefulness of CV ports].  

PubMed

Management of nutrition in cancer patients plays an important role in supporting anti-cancer treatment. Parenteral nutrition is considered to assist with nutrition in cancer patients. Central venous catheters(CVC)are useful for intravenous infusion of not only nutrients with high osmotic pressure but also chemotherapeutic drugs and other substances. Central venous access through CV ports reduces patient's burden and complications, and it contributes to maintaining a patient's quality of life(QOL). PMID:25335701

Washizawa, Naohiro; Yajima, Satoshi; Otsuka, Yuichiro; Koike, Junichi; Watanabe, Masashi; Kaneko, Hironori

2014-10-01

47

Ultrastructure and germination of Vitis vinifera cv. Loureiro pollen  

Microsoft Academic Search

Summary.  The cultivar Loureiro of Vitis vinifera is one of the most economically important, recommended in almost the totality of the Região Demarcada dos Vinhos Verdes.\\u000a In vineyards, the grape productivity of this cultivar is normal while in others it is extremely low. The aim of this work\\u000a was to study the morphology and germination of Vitis vinifera cv. Loureiro pollen

I. Abreu; I. Costa; M. Oliveira; M. Cunha; R. de Castro

2006-01-01

48

Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China.  

PubMed

Pigeon circovirus (PiCV) is one of four viruses in the family Circoviridae that affect young pigeons around the world. We collected 158 serum or tissue samples from six poultry farms in eastern China to investigate the prevalence and genetic characteristics of PiCV in Chinese pigeons. We tested for PiCV using a PCR assay and found that PiCV was present in 80.7 % (88/109) of diseased pigeons and 63.3 % (31/49) of healthy pigeons; overall, 75.3 % (119/158) of samples were PiCV positive. One PiCV-positive sample from each poultry farm was randomly chosen for amplification of the complete PiCV genome by inverse primer PCR (IP-PCR). The six genomic PiCV strains were designated as AHBZ (KJ704801), HBLF-E2 (KJ704802), JSJN (KJ704803), NJPK-21 (KJ704804), SDDZ (KJ704805) and SHWH-AB4 (KJ704806). We compared these new PiCV genomes to six publicly available PiCV genomes and found that the Rep and Cap genes had sequence identity ranging from 93.8 % to 100 % and 79.1 % to 100 %, respectively. In a phylogenetic analysis, PiCV and eight other members of the genus Circovirus were sister to chicken anemia virus (CAV), the only member of genus Gyrovirus. The results of this study provide evidence that PiCV is present in Chinese pigeons at a high rate and that PiCV is a viral lineage that is distinct from CAV. PMID:25348272

Zhang, Zhicheng; Dai, Wei; Wang, Shaohui; Dai, Dingzhen

2015-01-01

49

Callus formation from Malus x domestica cv. 'Jonathan' protoplasts.  

PubMed

Protoplasts could be successfully isolated and cultured from callus and suspension cultures of Malus xdomestica cv. 'Jonathan'. Protoplast-derived colonies were recovered when the osmoticum (glucose) was gradually reduced in semi-solid 8p medium or by the use of feeder plates. Formation of embryo-like structures was induced from the protoplast-derived callus on media supplemented with IAA and BA. These structures formed roots but plants failed to develop. Protoplasts could be isolated from leaves, but not from stems or petioles. The leaf protoplasts failed to divide. PMID:24253471

Kouider, M; Hauptmann, R; Widholm, J M; Skirvin, R M; Korban, S S

1984-08-01

50

Magnetic CVs in the UCT CCD CV Survey  

E-print Network

An overview is given of all the magnetic CVs found in the UCT CCD CV Survey (Woudt & Warner 2001, 2002, 2003a). We have identified eight new candidate Intermediate Polars (IP), of which six are classical novae (RR Cha, DD Cir, AP Cru, V697 Sco, V373 Sct, and RX J1039.7-0507). The two other candidate IPs are Aqr1 (2236+0052) and RX J0944.5+0357. In addition, there are two probable Polars, namely V351 Pup (= Nova Puppis 1991) and FIRST J102347.6+003841.

Woudt, P A; Woudt, Patrick A.; Warner, Brian

2003-01-01

51

Properties of NAC and CV[NAC] for Energy Models  

E-print Network

for many linear energy models of the form E = a + bT and a large range of temperature data sets. Define TL, as in Fels [1], to be the long-term average temperature (30 years is recommended). Then recall that the parameters a, b, and NAC for the model E = a...ESL-TR-93/05-03 Properties of CV [NAC] for Linear Energy Models David K. Ruch Sam Houston State University k Energy Systems Laboratory May 1993 1. Introduction and Notation The stability of the NAC estimate for the PRISM model has been shown em...

Ruch, D. K.

1993-01-01

52

Odour-active compounds in banana fruit cv. Giant Cavendish.  

PubMed

Application of solid-phase microextraction, simultaneous distillation-extraction and liquid-liquid extraction, combined with GC-FID, GC-MS, aroma extract dilution analysis, and odour activity value were used to analyse volatile compounds from banana fruit cv. Giant Cavendish and to estimate the most odour-active compounds. The analyses led to the identification of 146 compounds, 124 of them were positively identified. Thirty-one odourants were considered as odour-active compounds and contribute to the typical banana aroma, eleven of them are reported for the first time as odour-active compounds. PMID:23790849

Pino, Jorge A; Febles, Yanet

2013-11-15

53

Odour-active compounds in papaya fruit cv. Red Maradol.  

PubMed

Application of solid-phase microextraction and simultaneous distillation-extraction combined with GC-FID, GC-MS, aroma extract dilution analysis, and odour activity value were used to analyse volatile compounds from papaya fruit cv. Red Maradol and to estimate the most odour-active compounds. The analyses led to the identification of 137 compounds; 118 of them were positively identified. Twenty-five odorants were considered as odour-active compounds and contribute to the typical papaya aroma, from which ethyl butanoate, benzyl isothiocyanate, 1-hexen-3-one, (E)-?-ionone, and methyl benzoate were the most odour-active compounds. PMID:24176322

Pino, Jorge A

2014-03-01

54

Some physical, pomological and nutritional properties of kiwifruit cv. Hayward.  

PubMed

In this research, several physical, pomological and nutritional properties that are important for the design of equipments for harvesting, processing, transportation, sorting, separation and packaging of kiwifruit cv. Hayward grown in the Black Sea region of Turkey were determined. The fruit characteristics ranged from 72.28 g for average fruit weight, 59.41, 46.28 and 42.87 mm for fruit length, width and thickness, 49.03 mm for the geometric mean diameter, 0.825% for sphericity and 66.52 cm(3) for the volume of fruit, respectively. The bulk density, fruit density and porosity were determined as 575.27 kg/m(3), 1,093 kg/m(3) and 47.13%. The highest coefficient of static friction was obtained on plywood as 0.190, followed by polyethylene, rubber and galvanized steel sheet as 0.173, 0.163 and 0.158, respectively. The total soluble solid content, acidity, vitamin C, ash and total nitrogen content of kiwifruit cv. Hayward were 7.32%, 1.64%, 108 mg/100g, 0.71 g/100 g and 0.84%, respectively. The fresh fruits have 1.09 mg/100g total chlorophylls and flesh color data represented as L, a and b were 57.18, 17.25 and 37.46, respectively. PMID:17710584

Celik, Ahmet; Ercisli, Sezai; Turgut, Nihat

2007-09-01

55

The All-Sky Automated Survey for Supernovae CV Patrol  

NASA Astrophysics Data System (ADS)

Even in the modern era, only human eyes scan the entire optical sky for the violent, variable, and transient events that shape our universe. The "All Sky Automated Survey for Supernovae" (ASAS-SN or "Assassin") is changing this by monitoring the extra-galactic sky down to V~17 mag every 2-3 days using multiple telescopes, hosted by Las Cumbres Observatory Global Telescope Network, in the northern and southern hemispheres. By far the most common events observed by ASAS-SN are the Galactic transients. Since April 2013 ASAS-SN has identified over 180 new cataclysmic variable stars and announced over 260 new outbursts of known CVs. To make our data available to the CV community in 'real time', we have launched an automated 'CV Patrol' to monitor known CVs for outbursts as a useful tool for both professional and amateurs astronomers. It is a long term goal of ASAS-SN to make all our data public in real-time, and this patrol will serve as a framework for future ASAS-SN data releases.

Davis, Alexandra Bianca; Shappee, Benjamin John; Archer Shappee, Bartlett; ASAS-SN

2015-01-01

56

1 G. E. Bebout C.V. 05/13 Curriculum Vitae  

E-print Network

-86. Grove, M., Bebout, G. E., Jacobson, C. E., Barth, A. P., Kimbrough, D. L., King, R. L., Zou, H., Lovera1 G. E. Bebout C.V. ­ 05/13 Curriculum Vitae Gray E. Bebout, Professor Work Address: Department;2 G. E. Bebout C.V. ­ 05/13 Editing Projects Underway 2011 - Special issue of ELEMENTS (Geochemical

Bebout, Gray E.

57

Chondrule Magnetizations in the Allende CV Chondrite and Implications for the Dynamo of the CV Parent Body  

NASA Astrophysics Data System (ADS)

Many early-accreting planetesimals larger than several tens of km in diameter underwent extensive interior melting and differentiation. Advection in the molten metallic cores of these planetesimals may have generated magnetic dynamos. Remanent magnetization preserved in meteorites can reveal the past presence of core dynamo fields and therefore a metallic core on their parent bodies. Furthermore, the meteoritic magnetic record can constrain the duration of the dynamo, providing insight into the thermal evolution of the parent planetesimal. Carporzen et al. (2011) argued that bulk samples of the Allende CV carbonaceous chondrite carry a unidirectional partial thermoremanent magnetization (pTRM) blocked up to ~290C. They interpreted this magnetization as recording a magnetic core dynamo on the CV parent body. However, the previous study provided no constraints on the duration of the dynamo and did not characterize the magnetic recording in each component of the Allende meteorite. We conducted paleomagnetic experiments on 23 mutually oriented individual Allende chondrules and matrix samples. We also studied mutually oriented subsamples of 9 of these chondrules. We found that Allende chondrules can be divided into two distinct classes based on their natural remanent magnetization (NRM). Class A chondrules carry a strong low temperature overprint parallel to that of bulk Allende and matrix material that also unblocks at ~290C. Class B chondrules do not carry this low temperature overprint and exhibit randomly oriented NRMs. Electron microprobe analysis and thermal demagnetization of saturation remanence showed that magnetic phases in both Class A and Class B chondrules are likely products of parent body metasomatism. We infer that the random magnetization of Class B chondrules as well as the magnetization blocked above 290C in Class A chondrules and matrix material is a chemical remanent magnetization that resulted in randomly oriented remanence at the sub-millimeter scale. Allende chondrules therefore do not carry pre-accretional magnetization. The presence of a unidirectional pTRM overprint in Class A chondrules and matrix material but not in Class B chondrules requires a process that removed the pTRM overprint in Class B chondrules after its acquisition. We infer that, while all Allende components experienced metasomatic recrystallization of their magnetic phases, Class A chondrules completed this process before the pTRM event while Class B chondrules underwent metasomatism after the pTRM event. Furthermore, the random magnetization directions of Class B chondrules may suggest that a stable dynamo field no longer existed during the period of aqueous alteration, thereby constraining the lifetime of the CV parent body dynamo to less than several tens of My.

Weiss, B. P.; Fu, R. R.

2012-12-01

58

Solution NMR Structure of Hypothetical Protein CV_2116 Encoded by a Viral Prophage Element in Chromobacterium violaceum  

SciTech Connect

CV{_}2116 from Chromobacterium violaceum is a small hypothetical protein of 82 amino acids. A PSI-BLAST search using the CV{_}2116 protein sequence as a query identified only two hits, both with amino acid sequence identities of less than 40%. After the CV{_}2116 gene was cloned into the p15TvLic expression plasmid and transformed into E. coli, the desired CV{_}2116 protein was expressed and purified. A high quality solution structure of CV{_}2116 was determined by NMR spectroscopy. The tertiary structure of CV{_}2116 adopts a novel alpha + beta fold containing two anti-parallel beta sheets and one alpha helix in the C-terminal end. CV{_}2116 does not belong to any known protein sequence families and no similar structures exist in the protein data bank. To date, no function of CV{_}2116 can be derived from either sequence or structural similarity searches.

Yang, Yunhuang; Ramelot, Theresa A.; Cort, John R.; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl H.; Kennedy, Michael A.

2012-06-14

59

Seed Development in Phaseolus vulgaris L. cv Seminole  

PubMed Central

Phaseolus vulgaris L. cv Seminole pods removed from the plant continued their development when incubated in suitable conditions. Seeds continued to grow and develop and pods and seeds passed through an apparently normal developmental sequence to dryness. Seed growth was at the expense of pod dry weight (DW) reserves. Losses of pod DW paralleled DW gains by seeds in detached pods and in pod cylinders containing a seed. The transfer activity was apparent only within the period 10 to 30 days after anthesis (DAA) with maximal activity between 15 to 20 DAA. This period corresponds to maximum pod growth and the attainment of maximal DW. Seeds are in only the early phase of seed growth at this time. No DW transfer was observed at developmental stages beyond 30 to 35 DAA when normal senescence DW losses in pods became evident and seeds were in the later phase of seed fill. Pods or pod cylinders remained green and succulent over the transfer period, later passing through yellowing and drying phases characteristic of normal development. DW transfer was dependent on funicle integrity and was readily detectable in pod cylinders after 7 days incubation. The DW transfer activity may contribute to continuing nutrition of seeds under conditions where the normal assimilate supply to seeds becomes limiting. Defoliation and water stress treatments applied to Phaseolus plants reduced seed yields but allowed persistence of seed maturation processes such that all seeds developing to dryness were capable of germination. PMID:16666535

Fountain, David W.; Outred, Heather A.; Holdsworth, Jacqueline M.; Thomas, Roderick G.

1989-01-01

60

Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) Somaclones  

NASA Technical Reports Server (NTRS)

The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.

Campbell, W. F.; Carman, J. G.; Hashim, Z. N.

1990-01-01

61

THE BANANA PROJECT. V. MISALIGNED AND PRECESSING STELLAR ROTATION AXES IN CV VELORUM  

E-print Network

As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find ...

Albrecht, Simon

62

Full split C-V method for parameter extraction in ultra thin BOX FDSOI MOS devices  

NASA Astrophysics Data System (ADS)

The feasibility of full split C-V method in ultra-thin body and BOX (UTBB) FDSOI devices is demonstrated, emphasizing the usefulness of gate-to-bulk capacitance. The split C-V measurements carried out on both gate-to-channel and gate-to-bulk mode are shown to be consistent with TCAD simulation. This enabled us to propose an improved parameter extraction methodology for the whole vertical FDSOI stack from gate to substrate using back biasing effect.

Shin, Minju; Shi, Ming; Mouis, Mireille; Cros, Antoine; Josse, Emmanuel; Kim, Gyu-Tae; Ghibaudo, Gérard

2014-09-01

63

Chamobtusin A, a novel skeleton diterpenoid alkaloid from Chamaecyparis obtusa cv. tetragon.  

PubMed

The novel diterpenoid alkaloid chamobtusin A (1) was isolated from the branches and leaves of Chamaecyparis obtusa cv. tetragon. Its structure and relative stereochemistry were mainly determined by MS, 2D NMR, and X-ray methods. The methanol extracts, total alkaloids of C. obtusa cv. tetragon, and chamobtusin A were tested for their cytotoxicities against A549 and K562 human tumor cell lines. PMID:17894506

Zhang, Yu-Mei; Tan, Ning-Hua; Lu, Yang; Chang, Ying; Jia, Rui-Rui

2007-10-25

64

1 G. E. Bebout C.V. 9/14 Curriculum Vitae  

E-print Network

on ScienceDirect), p. 39-86. Grove, M., Bebout, G. E., Jacobson, C. E., Barth, A. P., Kimbrough, D. L., King1 G. E. Bebout C.V. ­ 9/14 Curriculum Vitae Gray E. Bebout, Professor Work Address: Department Cartigny, IPGP, Paris). This issue was published in Octo- ber, 2013. #12;2 G. E. Bebout C.V. ­ 9

Bebout, Gray E.

65

Detoxification of microcystin-LR in water by Portulaca oleracea cv.  

PubMed

Microcystin-LR (0.02 ?g/ml) in the hydroculture medium of Portulaca oleracea cv., became below the detection level (<0.0001 ?g/ml) by HPLC analysis after 7 days. The toxicity of microcystin estimated with protein phosphatase inhibition assay, however, remained at 37% of the initial level, indicating that microcystin-LR was transformed by P. oleracea cv. into unknown compound(s) of lower toxicity. PMID:23999063

Isobe, Takatoshi; Okuhata, Hiroshi; Miyasaka, Hitoshi; Jeon, Bong-Seok; Park, Ho-Dong

2014-03-01

66

Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa.  

PubMed

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution. PMID:25518947

Garigliany, Mutien-Marie; Hagen, Ralf Matthias; Frickmann, Hagen; May, Jürgen; Schwarz, Norbert Georg; Perse, Amanda; Jöst, Hanna; Börstler, Jessica; Shahhosseini, Nariman; Desmecht, Daniel; Mbunkah, Herbert Afegenwi; Daniel, Achukwi Mbunkah; Kingsley, Manchang Tanyi; de Mendonca Campos, Renata; de Paula, Vanessa Salete; Randriamampionona, Njary; Poppert, Sven; Tannich, Egbert; Rakotozandrindrainy, Raphael; Cadar, Daniel; Schmidt-Chanasit, Jonas

2014-01-01

67

Properties of a cationic peroxidase from Citrus jambhiri cv. Adalia.  

PubMed

The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatography on diethylaminoethanol-cellulose, carboxymethyl-cellulose, and Sephacryl S-200 columns. Cationic peroxidase POII is proved to be pure, and its molecular weight was 56 kDa. A study of substrate specificity identified the physiological role of POII, which catalyzed the oxidation of some phenolic substrates in the order of o-phenylenediamine > guaiacol > o-dianisidine > pyrogallol > catechol. The kinetic parameters (K (m), V (max), and V (max)/K (m)) of POII for hydrolysis toward H2O2 and electron donor substrates were studied. The enzyme had pH and temperature optima at 5.5 and 40 degrees C, respectively. POII was stable at 10-40 degrees C and unstable above 50 degrees C. The thermal inactivation profile of POII is biphasic and characterized by a rapid decline in activity on exposure to heat. The most of POII activity (70-80%) was lost at 50, 60, and 70 degrees C after 15, 10, and 5 min of incubation, respectively. Most of the examined metal ions had a very slight effect on POII except of Li+, Zn2+, and Hg2+, which had partial inhibitory effects. In the present study, the instability of peroxidase above 50 degrees C makes the high temperature short time treatment very efficient for the inactivation of peel peroxidase contaminated in orange juice to avoid the formation of off-flavors. PMID:18633734

Mohamed, Saleh A; El-Badry, Mohamed O; Drees, Ehab A; Fahmy, Afaf S

2008-08-01

68

Evaluation of antitranspirants on creeping bentgrass (Agrostis palustris Huds., cv. 'Penncros') and bermudagrass (Cynodon dactylon (L.) Pers. X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway')  

E-print Network

on red fescue (Festuca rubra L. cv. S-59) (3). PMA decreased transpiration rates 20/ by reducing stomatal aperture, with- out reducing the shoot growth rate, but with an increase of 1. 6 C in leaf temperature. Applications of PMA at this concentration...

Stahnke, Gwen Kristine

1981-01-01

69

CV-Muzar - The Virtual Community Environment that Uses Multiagent Systems for Formation of Groups  

NASA Astrophysics Data System (ADS)

The purpose of this chapter is to present two agents' societies responsible for group formation (sub-communities) in CV-Muzar (Augusto Ruschi Zoobotanical Museum Virtual Community of the University of Passo Fundo). These societies are integrated to execute a data mining classification process. The first society is a static society that intends preprocessing data, investigating the information about groups in the CV-Muzar. The second society is a dynamical society that will make a classification process by analyzing the existing groups and look for participants that have common subjects in order to constitute a sub-community. The formation of sub-communities is a new functionality within the CV-Muzar that intends to bring the participants together according to two scopes: interest similarity and knowledge complementarities.

de Marchi, Ana Carolina Bertoletti; Moraes, Márcia Cristina

70

An amoeboid olivine inclusion (AOI) in CK3 NWA 1559, comparison to AOIs in CV3 Allende, and the origin of AOIs in CK and CV chondrites  

NASA Astrophysics Data System (ADS)

An amoeboid olivine inclusion in CK3 NWA 1559 (0.54 × 1.3 mm) consists of a diopside-rich interior (approximately 35 vol%) and an olivine-rich rim (approximately 65 vol%). It is the first AOI to be described in CK chondrites; the apparent paucity of these inclusions is due to extensive parent-body recrystallization. The AOI interior contains irregular 3-15 ?m-sized Al-bearing diopside grains (approximately 70 vol%), 2-20 ?m-sized pores (approximately 30 vol%), and traces of approximately 2 ?m plagioclase grains. The 75-160 ?m-thick rim contains 20-130 ?m-sized ferroan olivine grains, some with 120º triple junctions. A few coarse (25-50 ?m-sized) patches of plagioclase with 2-18 ?m-thick diopside rinds occur in several places just beneath the rim. The occurrence of olivine rims around AOI-1 and around many AOIs in CV3 Allende suggests that CK and CV AOIs formed by the acquisition of porous forsteritic rims around fine-grained, rimless CAIs that consisted of diopside, anorthite, melilite, and spinel. Individual AOIs in carbonaceous chondrites may have formed after transient heating events melted their olivine rims as well as portions of the underlying interiors. In AOI-1, coarse plagioclase grains with diopside rinds crystallized immediately below the olivine rim. Secondary parent-body alteration transformed forsterite in the rims of CV and CK AOIs into more-ferroan olivine. Some of the abundant pores in the interior of AOI-1 may have formed during aqueous alteration after fine-grained melilite and anorthite were leached out. Chondrite groups with large chondrules tend to have large AOIs. AOIs that formed in dust-rich nebular regions (where CV and CK chondrites later accreted) tend to be larger than AOIs from less-dusty regions.

Rubin, Alan E.

2013-03-01

71

New parameter extraction method based on split C-V measurements in FDSOI MOSFETs  

NASA Astrophysics Data System (ADS)

A new parameter extraction methodology based on split C-V is proposed for FDSOI MOS devices. To this end, a detailed capacitance theoretical analysis is first conducted emphasizing the usefulness of the Maserjian function. Split C-V measurements carried out on various FDSOI CMOS technologies show that the Maserjian function exhibits a power law dependence with inversion charge as ?Qi-2 whatever the carrier type and gate oxide thickness. This feature enables to confirm the validity of a two-parameter simple capacitance model and allows for a reliable MOSFET parameter extraction in FDSOI devices.

Ben Akkez, Imed; Cros, Antoine; Fenouillet-Beranger, Claire; Boeuf, Frederic; Rafhay, Q.; Balestra, Francis; Ghibaudo, Gérard

2013-06-01

72

Refined purification of large amounts of rat cvHsp/HspB7 and partial biological characterization in vitro.  

PubMed

The cardiovascular heat shock protein (cvHsp/HspB7) exhibited cardiac-specific expression and is a possible candidate of dilated cardiomyopathy in heart failure. The molecular characteristics and biochemical properties of cvHsp are only partially understood. This study was aimed to identify the biological properties and molecular high-order structure of cvHsp. The cvHsp protein was prepared by the refined purification at large amount. The pooled fractions were existed as two types of oligomers in solution and exhibited chaperone-like activity. The circular dichroism analyzed ureainduced unfolding processes. Multiple sequence alignment and an automated protein modeling were used to describe the three-dimensional structural model of the cvHsp monomer and dimer. By the refined purification, the cvHsp appeared in oligomeric and dimeric forms (approximately 17 kDa and 40 kDa, respectively) composed of 18.6-kDa monomers. The cvHsp prevented dithiothreitol (DTT)-induced aggregation of the insulin B chain and conferred oligomeric unfolding process in urea-containing solution. It exhibited structural stability and conformed to the two-state folding/unfolding oligomerization model. According to sequence alignment of the rat cvHsp gene, three-dimensional model based on the crystallographic structure of wheat Hsp16.9 was reconstructed. The cvHsp presented two antiparallel ?-sheet sandwich structure of sHsp' core ?-crystallin domain, and formed dimeric or oligomeric organization in solution. This work described the structural components of cvHsp and existed as the polydispersed molecular oligomers in vitro, which are some common properties of the sHsp family. These characteristics of the cvHsp gene is helpful to clarify molecular functionality in cardiac diseases. PMID:24555434

Lin, Wei; Yang, Zehong; Lu, Yongzhi; Zhao, Xiaojun

2014-05-01

73

The chemical composition of suberin in apoplastic barriers affects radial hydraulic conductivity differently in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix)  

Microsoft Academic Search

Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degrada- tion (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were ana- lysed quantitatively by gas chromatography and mass spectrometry. In corn, suberin was determined for isolated endodermal (ECW) and rhizo-hypodermal (RHCW) cell

Lukas Schreiber; Rochus Franke; Klaus-Dieter Hartmann; Kosala Ranathunge; Ernst Steudle

2005-01-01

74

CV-SLAM: a new ceiling vision-based SLAM technique  

Microsoft Academic Search

We propose a fast and robust CV-SLAM (ceiling vision-based simultaneous localization and mapping) technique using a single ceiling vision sensor. The proposed algorithm is suitable for system that demands very high localization accuracy such as an intelligent robot vacuum cleaner. A single camera looking upward direction (called ceiling vision system) is mounted on the robot, and salient image features are

WooYeon Jeong; Kyoung Mu Lee

2005-01-01

75

Dr. Matas Alvarado (CV breve) Investigador Titular del Departamento de Computacin del CINVESTAV; Miembro Regular de  

E-print Network

Dr. Matías Alvarado (CV breve) Investigador Titular del Departamento de Computación del CINVESTAV. JOS� MATÍAS ALVARADO MENTADO Curriculum Vitae DATOS PROFESIONALES Investigador CINVESTAV 3B (Febrero JCR ISI 1. Alvarado Matias, Sheremetov, L., Bañares, R. & Cantú, F. (Guest Eds.) Special Issue

Alvarado, Matías

76

Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp. trichophorum  

Microsoft Academic Search

A partial amphiploid, TE-3, between Triticum aestivum cv. Chinese Spring (CS) and Thinopyrum intermedium ssp. trichophorum was characterized by cytological observation, genomic in situ hybridization (GISH), seed storage protein electrophoresis and disease resistance screening. The TE-3 plants were deeply covered with pubescence, which is characteristic of the Th. intermedium ssp. trichophorum parent. Feulgen staining of the somatic metaphases revealed that

Yang Zu-Jun; Li Guang-Rong; Chang Zhi-Jian; Zhou Jian-Ping; Ren Zheng-Long

2006-01-01

77

Simple Cp/Cv Resonance Apparatus Suitable for the Physics Teaching Laboratory.  

ERIC Educational Resources Information Center

Describes a resonance apparatus for the measurement of Cp/Cv for different gases. In the apparatus a magnetically supported piston in a vertical cylindrical glass tube containing the gas is forced into oscillation by means of a standard audio signal generator. (Author/GA)

Smith, D. G.

1979-01-01

78

Kenmerk: CvB 2008/879 Afspraken rond invoering UFO  

E-print Network

Kenmerk: CvB 2008/879 Afspraken rond invoering UFO hulpinstrument voor secretaressefuncties Rond de invoering van een UFO hulpinstrument voor de indeling van secretaressefuncties hebben het College van vergemakkelijken. Op de beleids- en uitvoeringsafspraken van UFO heeft dit geen effect; het is een additioneel

Franssen, Michael

79

J.A. Lewis CV updated 9.6.11 JENNIFER A. LEWIS  

E-print Network

of Leeds, Leeds, England; Department of Ceramic Engineering, Summer Intern 1985-1986 University of IllinoisJ.A. Lewis CV ­ updated 9.6.11 1 JENNIFER A. LEWIS Curriculum Vitae Rm. 2015 MRL Phone: (217) 244 Professor, University of California at Santa Barbara (sabbatical leave) Summer 1996 Visiting Scientist

Shim, Moonsub

80

J.A. Lewis CV updated 3.3.12 JENNIFER A. LEWIS  

E-print Network

of Leeds, Leeds, England; Department of Ceramic Engineering, Summer Intern 1985-1986 University of IllinoisJ.A. Lewis CV ­ updated 3.3.12 1 JENNIFER A. LEWIS Curriculum Vitae Rm. 2015 MRL Phone: (217) 244 Professor, University of California at Santa Barbara (sabbatical leave) Summer 1996 Visiting Scientist

Weaver, John H.

81

Indirect measurements of Brachiaria brizantha cv. Marandu fermentable cell wall sugars for second generation biofuels production.  

Technology Transfer Automated Retrieval System (TEKTRAN)

Results of a study conducted to evaluate the possibility of using IVDMD values of B. brizantha cv. Marandu to predict cell wall sugars that would be available in a biorefinery for ethanol production are reported. The study was conducted based on the similarity between rumen enzymes and those used i...

82

Thermal degradation of anthocyanins from purple potato (Cv. Purple Majesty) and their impact on antioxidant capacity  

Technology Transfer Automated Retrieval System (TEKTRAN)

Degradation parameters of purified anthocyanins from purple-fleshed potato (Purple Majesty cv.) heated at high temperatures (100 - 150 °C) was determined. Purified anthocyanins, prepared by removing salts, sugars and colorless non-anthocyanin phenolics from the crude extract, were quantified using H...

83

Substance of green waste compost adding bamboo vinegar as growth stimulator for Calathca rotundifola cv. Fasciata  

Microsoft Academic Search

The composting process allows the conversion of green waste into plant substance that can be used in replacing Pindstrup Sphagnum (PS) for Calathca rotundifola cv. Fasciata cultivation. To help address this need, this study used bacterial reagent and bamboo vinegar as additives in composting process, and chosen the best green waste compost (BGWC) with the best Physico-chemical properties forpartly or

Yun Tian; Xiangyang Sun; Jixin Cao; Lu Zhang; Baozhu Zhang

2011-01-01

84

Modeling the structure of CV formation and expectations : The commercial retail real estate sector  

Microsoft Academic Search

Purpose – The paper aims to form system dynamics modeling in introduced in conjunction with econometric analysis and planned scenario analysis which will uniquely structure the process whereby the ex ante capital values of the prime retail real estate sector. Design\\/methodology\\/approach – The integrated system dynamics model investigates the structural factors affecting a unique expectation-centered capital value (CV) formation of

2007-01-01

85

Alteration processes in the CV chondrite parent body based on analysis of NWA 2086 meteorite  

NASA Astrophysics Data System (ADS)

Analysis of the NWA 2086 CV3 chondrite showed a matrix/chondrule ratio of 52%, similar to Bali, Mokoia, and Grosanaja. Nearly twice as many chondrule fragments as intact ones demonstrate that an early fragmentation phase occurred prior to final accretion. After this event, no substantial mechanical change or redeposition is evident. Rims with double-layered structures were identified around some chondrules, which, in at least one case, is attributed to an accretionary origin. The rim's outer parts with a diffuse appearance were formed by in situ chemical alteration. During this later process, Mg content decreased, Fe content increased, and olivine composition was homogenized, producing a rim composition close to that of the matrix. This alteration occasionally happened along fractures and at confined locations, and was probably produced by fluid interactions. Iron oxides are the best candidate for a small grain-sized alteration product; however, technical limitations in the available equipment did not allow exact phase identification. These results suggest that NWA 2086 came from a location (possible more deeply buried) in the CV parent body than Mokoia or Bali, and suffered less impact effects—although there is no evidence of sustained thermal alteration. This meteorite may represent a sample of the CV parent asteroid interior and provide a useful basis for comparison with other CV meteorites in the future.

Kereszturi, A.; Blumberger, Z.; Józsa, S.; May, Z.; Müller, A.; Szabó, M.; Tóth, M.

2014-08-01

86

Desiati CV 1011 1 Publications in Refereed Journals as a contributing author  

E-print Network

Desiati CV 1011 1 Publications in Refereed Journals as a contributing author 1. P. Desiati and L to ApJ (arXiv:1109.1017). Contributing author with Rasha Abbasi. 4. IceCube collaboration, Observation with IceCube, ApJ 718 L194 (2010). Contributing author with Rasha Abbasi. 9. (with J. Lundberg, et al

Sheridan, Jennifer

87

EST-SSR MARKERS FROM FRAGARIA VESCA L. CV. YELLOW WONDER  

Technology Transfer Automated Retrieval System (TEKTRAN)

Fourteen microsatellite primer pairs were developed from a cDNA library of Fragaria vesca cv. Yellow Wonder. Transferability to 13 species of Fragaria ranged from 71% in diploid species F. gracilis Losinsk., F. iinumae Makino, F. nilgerrensis Schltdl. ex J. Gay and F. nipponica Makino to 100% in the...

88

Phytoremediation of 4,4'-thiodiphenol (TDP) and other bisphenol derivatives by Portulaca oleracea cv.  

PubMed

4,4'-Thiodiphenol (TDP) is a bisphenol derivative, and there has been no report on TDP removal by any plants or pure bacterial cultures. The removal of TDP by Portulaca oleracea cv., a floricultural herbal plant, was examined with a hydroculture system, and 97% of TDP was removed after 4 days culture. PMID:23040992

Okuhata, Hiroshi; Ninagawa, Masahiko; Takemoto, Naomichi; Ji, Hezhe; Miyasaka, Hitoshi; Iwamoto, Ai; Nagae, Masaki; Ishibashi, Yasuhiro; Arizono, Koji

2013-01-01

89

RESEARCH INTERESTS PATRICK A. ZOLLNER Note numbers refer to papers in my CV  

E-print Network

abilities of eastern chipmunks, gray squirrels, and fox squirrels, and found that such abilities to papers in my CV Behavioral Ecology of Ecological Landscapes ­ Recent advances in landscape-level ecological modeling rely upon a rarely acknowledged and often unappreciated behavioral "standard

Fernández-Juricic, Esteban

90

Defects left after regrowth of amorphous silicon on crystalline Si : C(V) and DLTS studies  

E-print Network

29 Defects left after regrowth of amorphous silicon on crystalline Si : C(V) and DLTS studies J.10J 1. Introduction. Solid phase epitaxial (SPE) regrowth of silicon amorphized by ion implantation-Si et p-Si. Abstract. 2014 n and p-type silicon have been self-ion implanted at 77 K with multi

Boyer, Edmond

91

DNA dodecaploid plants detected among somaclones of Actinidia deliciosa var. deliciosa cv. Hayward  

Microsoft Academic Search

Summary  Ninety two adventitious shoots (somaclones) were regenerated from leaf-derived callus of Actinidia deliciosa var. deliciosa cv. Hayward. Each somaclone was multiplied in vitro through axillary bud culture to produce several individual clonal shoots. A total of 671 shoots were exflasked but only 223 (33%) resulted in rooted plants. These represented 46 of the original 92 somaclones. Shoot apices from representative

M. R. Boase; M. E. Hopping

1995-01-01

92

Measurement of band offsets and interface charges by the C-V matching method  

NASA Astrophysics Data System (ADS)

The present article describes a novel application of capacitance-voltage measurements to determine simultaneously the band discontinuities (?EV, ?EC) and interface charge density (?) of heterojunctions. The method, which we refer to as C-V matching, complements the most versatile C-V profiling technique proposed by Kroemer and successfully applied by others. In contrast to the C-V profiling which is limited to isotype heterojunctions, the new method is applicable to p-n heterojunctions as well. The methodology is based on three cardinal equations which are not controversial—the lineup of the bands relative to the common Fermi level (at equilibrium) or the quasi-Fermi levels (when voltage is applied), the charge neutrality and the expression for the total capacitance of the heterostructure. The three equations are formulated for equilibrium as well as nonequilibrium conditions, using quasi-Fermi levels and the quasi-equilibrium approximation. The three cardinal equations are defined by the two constant (albeit unknown) interface parameters (?EV,?) which are assumed to be independent of the voltage and two variables (?s1, ?s2), which describe the total band bending on each side of the heterointerface and vary with the applied voltage. The actual interface parameters ?EV, ? are determined by C-V matching between the calculated and the measured curve. The metric for the optimal match between calculated and measured capacitance vectors is discussed. The methodology presented in this study is general and can be applied to semiconductor-semiconductor and semimetal-semiconductor heterojunctions. It is illustrated here for the HgTe-CdTe semimetal-semiconductor heterojunction, which cannot be evaluated by the C-V profiling. The significance of the simultaneous determination of the band discontinuities and interface charges of heterojunctions is also discussed. In addition, the methodology presented in this article models the behavior of biased heterojunctions under nonequilibrium conditions, taking into consideration the values of band offset and interface charge density of an actual heterointerface.

Nemirovsky, Y.; Gordon, G.; Goren, D.

1998-07-01

93

Effects of CV-3611, a new free radical scavenger, on ischemic heart failure in conscious beagle dogs.  

PubMed

The effects of CV-3611, a new free radical scavenger, on coronary circulation failure and infarct size after ischemia/reperfusion were studied in conscious beagle dogs. The dogs underwent occlusion of the left circumflex coronary artery for 60 min and then were reperfused for 14 days. The dogs were divided into three groups: a control group, a pre-treated group that received CV-3611 or alpha-tocopherol, and a post-treated group that received CV-3611. During occlusion, varying degrees of ventricular arrhythmia were noted; after reperfusion, the arrhythmia tended to become severe. CV-3611 at a daily dose of 10 mg/kg or 30 mg/kg and alpha-tocopherol at a daily dose of 60 mg/kg reduced the incidence of overall post-occlusion arrhythmia. Coronary blood flow in the control group was reduced to 20% of the preocclusion level at 7 days after reperfusion, whereas in the CV-3611 and alpha-tocopherol treated groups, the decreased coronary flow was remarkably suppressed. The infarct size for the CV-3611- and alpha-tocopherol-treated groups, measured at 14 days after reperfusion, was reduced by 70% when compared with the control group. Based on these observations, it is proposed that CV-3611 exerts its beneficial effects on ischemic tissue by protecting against oxygen free radical-mediated damage induced by ischemia/reperfusion. PMID:2779011

Tanabe, M; Kito, G

1989-08-01

94

Graphical method for determining the coefficient of consolidation cv from a flow-pump permeability test  

USGS Publications Warehouse

A graphical method has been developed for determining the coefficient of consolidation from the transient phases of a flow-pump permeability test. The flow pump can be used to infuse fluid into or withdraw fluid from a laboratory sediment specimen at a constant volumetric rate in order to obtain data that can be used to calculate permeability using Darcy's law. Representative type-curve solutions to the associated forced-flow and pressure-decay models are derived. These curves provide the basis for graphically evaluating the permeability k, the coefficient of consolidation cv, and the coefficient of volume change mv. The curve-matching technique is easy and rapid. Values of k, cv and mv for a laterally confined kaolinite specimen were determined by this graphical method and appear to be in reasonably good agreement with numerically derived estimates (within 20%). Discrepancies between the two sets of results seem to be largely a function of data quality.

Morin, Roger H.; Olsen, Harold W.; Nelson, Karl R.; Gill, James D.

1989-01-01

95

Phylogenetic position of Mesorhizobium huakuii subsp. rengei, a Symbiont of Astragalus sinicus cv. Japan  

Microsoft Academic Search

The phylogenetic position of Rhizobium huakuii bv. renge, a symbiont of Astragalus sinicus cv. Japan (renge-sou), was studied. The following phylogenetic approaches were used: restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR) analysis of a full-length 16S rDNA fragment, 16S rDNA analysis of the first 300-bp sequence, bacteriophage typing, and amplification of the genomic region by random primer. All the

Sukma Nuswantara; Makoto Fujie; Takashi Yamada; Wanda Malek; Masaki Inaba; Yoshinobu Kaneko; Yoshikatsu Murooka

1999-01-01

96

Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local  

Microsoft Academic Search

A protocol for plant regeneration by somatic embryogenesis was developed in guava cv. Banarasi local by using immature zygotic embryo explants. Best induction of somatic embryogenesis was achieved from 10-week-old zygotic embryos on MS medium supplemented with 2,4-d (4.52?M) and 5% sucrose. Maximum number of somatic embryos was produced when zygotic embryo explants were transferred to growth regulator free full

Manoj K. Rai; N. Akhtar; V. S. Jaiswal

2007-01-01

97

Development of the Portable Synchrotron MIRRORCLE-CV for High Precision Non-Destructive Testing  

SciTech Connect

We started the development of the portable synchrotron MIRRORCLE-CV series, which provides a high quality x-ray beam for high precision non-destructive testing (NDT). Computer simulations for the magnetic field design and electron dynamics reveal that the outer diameter of the synchrotron magnet can be as small as 30 cm. This synchrotron size approaches that of a conventional x-ray tube.

Hasegawa, Daisuke; Toyosugi, Norio; Noh, Young Deok; Yamada, Takanori; Morita, Masaki; Mantey, Edward; Masaoka, Sei [Photon Production Laboratory, Ltd., 4-2-1 (808) Takagai-cho Minami, Ohmihachiman-city 523-0898, Shiga (Japan); Yamada, Hironari [Synchrotron Light Life Science Center, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu-city 525-8577, Shiga (Japan)

2007-01-19

98

MIRRORCLE-CV The Portable Synchrotron For Precise Non-Destructive Testing And Medical Diagnosis  

SciTech Connect

We are developing the portable synchrotron MIRRORCLE-CV series, which provides a high quality x-ray beam for high precision non-destructive testing (NDT). Computer simulations for the magnetic field design and electron dynamics reveal that the outer diameter of the synchrotron magnet can be as small as 30 cm. This synchrotron size approaches that of a conventional x-ray tube.

Hasegawa, Daisuke [Photon Production Laboratory Ltd., 4-2-1 (808) Takagai-cho Minami, Ohmihachiman-city 523-0898, Shiga (Japan); Yamada, Hironari [Synchrotron Light Life Science Center, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu-city 525-8577, Shiga (Japan)

2007-03-30

99

Effects of Rooting Substrates on In Vitro Rooting of Anthurium andraeanum L. cv. Avanti  

Microsoft Academic Search

A study was made of the effects of rooting substrates on in vitro rooting of Anthurium andraeanum L. cv. Avanti, orange flower. Initiation of root was attempted in several rooting substrates with modified ½ MS medium supplemented with 30 g\\/l sucrose. The cut end of the shoot was dipped in 2.5 g\\/l indole-3-butyric acid (IBA) before insertion in substrates. After

Wararat KEATMETHA; Padungsak SUKSA-ARD

100

Direct Observation of Channel-Doping-Dependent Reverse Short Channel Effect Using Decoupled CV Technique  

Microsoft Academic Search

A non-destructive high resolution ``Decoupled C-V Technique'' for small geometry devices has been developed and demonstrated to successfully extract the intrinsic channel capacitance of submicron metal-oxide-semiconductor field effect transistors (MOSFET's). The effective channel doping concentration calculated from the extracted intrinsic gate capacitance presents an obvious dopant concentration enhancement in the intrinsic channel region of submicron devices compared to that of

Jyh-Chyurn Guo; Charles Ching-Hsiang Hsu; Steve Shao-Shiun Chung

1994-01-01

101

Efficient and Stable Transformation of Lactuca sativa L. cv. Cisco (lettuce) Plastids  

Microsoft Academic Search

Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However,\\u000a broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation\\u000a systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene

Hirosuke Kanamoto; Atsushi Yamashita; Hiroshi Asao; Satoru Okumura; Hisabumi Takase; Masahira Hattori; Akiho Yokota; Ken-Ichi Tomizawa

2006-01-01

102

Molecular and biochemical characterization of cytosolic phosphoglucomutase in wheat endosperm (Triticum aestivum L. cv. Axona)  

Microsoft Academic Search

Evidence from a number of plant tissues suggests that phosphoglucomutase (PGM) is present in both the cytosol and the plastid. The cytosolic and plasti- dic isoforms of PGM have been partially purified from wheat endosperm (Triticum aestivum L. cv. Axona). Both isoforms required glucose 1,6-bisphosphate for their activity with Ka values of 4.5 mM and 3.8 mM for cytosolic and

Emma J. Davies; Ian J. Tetlow; Caroline G. Bowsher; Michael J. Emes

2003-01-01

103

SALT spectral observations of a new, bright, southern CV: MASTER OT J142023.5-485540  

NASA Astrophysics Data System (ADS)

On 2013-06-24.063 UT, the Southern African Large Telescope (SALT; Buckley et al. 2006; O’Donoghue et al. 2006) observed MASTER OT J142023.5-485540 (RA=14h 20m 23.5s; Dec=-48d 55m 40s). This outbursting CV was discovered by MASTER-ICATE on 2013-06-08.048 UT and followup observations were requested in ATEL #5144.

Gulbis, A. A. S.; Kotze, M. M.; Kotze, E. J.; Worters, H. L.; Buckley, D. A. H.; O'Donoghue, H.; Shara, M.

2013-07-01

104

Extremely Na and Cl-rich chondrule from the CV3 carbonaceous chondrite Allende  

Microsoft Academic Search

We report on a study of Al3509, a large Na- and Cl-rich, radially-zoned object from the oxidized CV carbonaceous chondrite Allende. Al3509 consists of fine-grained ferroan olivine, ferroan Al-diopside, nepheline, sodalite, and andradite, and is crosscut by numerous veins of nepheline, sodalite, and ferroan Al-diopside. Some poorly-characterized phases of fine-grained material are also present; these phases contain no significant H2O.

G. J. Wasserburg; I. D. Hutcheon; J. Aléon; E. C. Ramon; A. N. Krot; K. Nagashima; A. J. Brearley

2011-01-01

105

Changes in leaf, stem, and root anatomy of Chrysanthemum cv. Lillian Hoek following paclobutrazol application  

Microsoft Academic Search

Plants ofChrysanthemum cv. Lillian Hoek were treated with a paclobutrazol (PBZ) soil drench and histologically examined after 3 months. PBZ application\\u000a resulted in thicker leaves, reduced stem diameter, and roots with an increased diameter and an unusual segmented appearance.\\u000a Increased leaf thickness was partly due to an additional layer of palisade mesophyll, although individual palisade cells were\\u000a shorter, of smaller

G. E. Burrows; T. S. Boag; W. P. Stewart

1992-01-01

106

Fayalite in the Vigarano CV3 carbonaceous chondrite: Occurrences, formation age and conditions  

Microsoft Academic Search

We have performed petrographic characterization, 53Mn–53Cr age determination and thermodynamic stability evaluations of fayalite in Vigarano meteorite that belongs to the reduced subgroup of CV3 chondrites. Vigarano is a breccia consisting of clasts which are separate chondrules surrounded by olivine-rich fine-grained materials. Four out of twenty three explored clasts contain fayalites that represent materials of the Bali-like oxidized subgroup of

Kaori Jogo; Tomoki Nakamura; Takaaki Noguchi; Mikhail Yu. Zolotov

2009-01-01

107

Genetic transformation of Cavendish banana ( Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment  

Microsoft Academic Search

An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature\\u000a male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or

D. K. Becker; B. Dugdale; M. K. Smith; R. M. Harding; J. L. Dale

2000-01-01

108

Anthelmintic activities of aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana.  

PubMed

Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana. PMID:24583851

Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

2014-01-01

109

MICHAEL KHL CV & publications April 2014 Personal data: Born 16-06-1964, Danish citizen, married, 2 daughters, 1 son  

E-print Network

MICHAEL KÃ?HL CV & publications April 2014 1 Personal data: Born 16-06-1964, Danish citizen, married. Copenhagen (A prestigious Danish Research Council research fellowship at the level of full professor). 1995

Kühl, Michael

110

Compositional and petrographic similarities of CV and CK chondrites: A single group with variations in textures and volatile concentrations attributable to impact heating, crushing and oxidation  

NASA Astrophysics Data System (ADS)

Greenwood et al. (2010) gathered data on O-isotopic and elemental compositions and reevaluated literature data for CV and CK chondrites. They concluded that these two chondrite groups originated on the same parent asteroid, with CK chondrites being metamorphosed CV chondrites (which are otherwise missing types 4 through 6). To test this interpretation we have gathered new instrumental neutron-activation-analysis (INAA) data for CV and CK chondrites and reexamined their petrographic features. The new INAA data like the older data show scatter attributable to weathering effects, but we conclude that the refractory lithophile abundances are the same in CV and CK, in agreement with the Greenwood et al. interpretation. Several volatile elements are significantly lower in CK than in CV chondrites. Among the elements we determine, the greatest difference between CV and CK is found for Br, for which the CV/CK ratio is ?4; As and Sb are about 20% lower in CK than CV and smaller differences are observed for Zn, Ga and Se. It seems likely that volatiles were lost during impact-heating events that also provided the heat responsible for metamorphic recrystallization. Within statistical uncertainty, chondrules in CV and CK chondrites are the same size and have similar textural distributions. A significant petrographic difference between CK and CV chondrites cited by Kallemeyn et al. (1991) was the much higher percentage of igneous rims around CV chondrules. However, we now recognize that many chondrules in CK3.8 NWA 1559 have igneous rims and in CK4 chondrites, igneous rims are recognizable by their associated sulfide-rich rings; there are no quantifiable CV-CK differences in igneous-rim abundances. We used Ca and Al maps to show that CK chondrites have CAI abundances similar to those of CV chondrites. It thus appears that there are no resolvable pre-metamorphic petrographic differences between CV and CK chondrites. We recommend that the “CK” designation be abandoned and that the CV group be acknowledged as spanning the range of petrologic types 3-6. We suggest that CK3 chondrites be designated CV3OxK. Most CK4 chondrites are highly fragmental; collisional crushing appears to be much more common than in CV chondrites. It seems likely that CK chondrites formed from reduced CV3 materials after the latter was impacted, buried, aqueously altered and annealed.

Wasson, John T.; Isa, Junko; Rubin, Alan E.

2013-05-01

111

The Parent Interview for Autism-Clinical Version (PIA-CV)A Measure of Behavioral Change for Young Children with Autism  

Microsoft Academic Search

The Parent Interview for Autism-Clinical Version (PIA-CV) was developed to measure autism symptom severity across a wide range of behavioral domains. Two studies were conducted to examine the psychometric properties of the PIA-CV for a sample of children under 3 years old. Results of study 1 revealed adequate internal consistency for nine of the 11 PIA-CV dimensions, as well as

Wendy L. Stone; Elaine E. Coonrod; Stacie L. Pozdol; Lauren M. Turner

2003-01-01

112

CDMRP (eReceipt) PI Guide to Funding G31cv1/ML/CDMRP PI Guide November 2009 Page 1 of 14  

E-print Network

CDMRP (eReceipt) PI Guide to Funding G31cv1/ML/CDMRP PI Guide November 2009 Page 1 of 14 INTERNAL://cdmrp.army.mil/ #12;CDMRP (eReceipt) PI Guide to Funding G31cv1/ML/CDMRP PI Guide November 2009 Page 2 of 14 Contents deadline. #12;CDMRP (eReceipt) PI Guide to Funding G31cv1/ML/CDMRP PI Guide November 2009 Page 3 of 14

113

Biochemical markers assisted screening of Fusarium wilt resistant Musa paradisiaca (L.) cv. puttabale micropropagated clones.  

PubMed

An efficient protocol was standardized for screening of panama wilt resistant Musa paradisiaca cv. Puttabale clones, an endemic cultivar of Karnataka, India. The synergistic effect of 6-benzyleaminopurine (2 to 6 mg/L) and thidiazuron (0.1 to 0.5 mg/L) on MS medium provoked multiple shoot induction from the excised meristem. An average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/L 6-benzyleaminopurine and 0.3 mg/L thidiazuron concentrations. Elongation of shoots observed on 5 mg/L BAP augmented medium with a mean length of 8.38 +/- 0.30 shoots per propagule. For screening of disease resistant clones, multiple shoot buds were mutated with 0.4% ethyl-methane-sulfonate and cultured on MS medium supplemented with Fusarium oxysporum f. sp. cubense (FOC) culture filtrate (5-15%). Two month old co-cultivated secondary hardened plants were used for screening of disease resistance against FOC by the determination of biochemical markers such as total phenol, phenylalanine ammonia lyase, oxidative enzymes like peroxidase, polyphenol oxidase, catalase and PR-proteins like chitinase, beta-1-3 glucanase activities. The mutated clones of M. paradisiaca cv. Puttabale cultured on FOC culture filtrate showed significant increase in the levels of biochemical markers as an indicative of acquiring disease resistant characteristics to FOC wilt. PMID:23898552

Venkatesh; Krishna, V; Kumar, K Girish; Pradeepa, K; Kumar, S R Santosh; Kumar, R Shashi

2013-07-01

114

Electroacupuncture Stimulation at CV12 Inhibits Gastric Motility via TRPV1 Receptor  

PubMed Central

Gastric dysmotility is one of the major pathophysiological factors in functional gastrointestinal disorders. Acupuncture, as one of the alternative approaches, is efficacious in the treatment of gastrointestinal motility disorders; however, the mechanism underlying its action is unclear. In the present study, we used both capsazepine, a TRPV1 antagonist, and TRPV1 knockout mice. Animals were divided into wild-type group (WT), capsazepine injection group (CZP, 0.5?mg/kg, i.p.), and TRPV1 knockout mice group (TRPV1?/?). Each of these three groups was divided into three subgroups, which were subjected to EA stimulation at acupoint Zhongwan (CV12) at a different intensity (1, 2, or 4?mA). We demonstrated that electroacupuncture at Zhongwan (CV12) markedly inhibited gastric motility at 2 and 4?mA in an intensity-dependent manner in wild-type mice. The inhibitory effect was also observed in capsazepine-injected and TRPV1?/? mice but was no longer intensity dependent, indicating that TRPV1 is partially involved in the electroacupuncture-mediated modulation of gastric motility. PMID:24106521

Yu, Zhi; Cao, Xin; Xia, Youbing; Ren, Binbin; Feng, Hong; Wang, Yali; Jiang, Jingfeng; Xu, Bin

2013-01-01

115

Heterogeneous compute in computer vision: OpenCL in OpenCV  

NASA Astrophysics Data System (ADS)

We explore the relevance of Heterogeneous System Architecture (HSA) in Computer Vision, both as a long term vision, and as a near term emerging reality via the recently ratified OpenCL 2.0 Khronos standard. After a brief review of OpenCL 1.2 and 2.0, including HSA features such as Shared Virtual Memory (SVM) and platform atomics, we identify what genres of Computer Vision workloads stand to benefit by leveraging those features, and we suggest a new mental framework that replaces GPU compute with hybrid HSA APU compute. As a case in point, we discuss, in some detail, popular object recognition algorithms (part-based models), emphasizing the interplay and concurrent collaboration between the GPU and CPU. We conclude by describing how OpenCL has been incorporated in OpenCV, a popular open source computer vision library, emphasizing recent work on the Transparent API, to appear in OpenCV 3.0, which unifies the native CPU and OpenCL execution paths under a single API, allowing the same code to execute either on CPU or on a OpenCL enabled device, without even recompiling.

Gasparakis, Harris

2014-02-01

116

Chemical and physical studies of type 3 chondrites 12: The metamorphic history of CV chondrites and their components  

NASA Technical Reports Server (NTRS)

The induced thermoluminescence (TL) properties of 16 CV and CV-related chondrites, four CK chondrites and Renazzo (CR2) have been measured in order to investigate their metamorphic history. The petrographic, mineralogical and bulk compositional differences among the CV chondrites indicate that the TL sensitivity of the approximately 130 C TL peak is reflecting the abundance of ordered feldspar, especially in chondrule mesostasis, which in turn reflects parent-body metamorphism. The TL properties of 18 samples of homogenized Allende powder heated at a variety of times and temperatures, and cathodoluminescence mosaics of Axtell and Coolidge, showed results consistent with this conclusion. Five refractory inclusions from Allende, and separates from those inclusions, were also examined and yielded trends reflecting variations in mineralogy indicative of high peak temperatures (either metamorphic or igneous) and fairly rapid cooling. The CK chondrites are unique among metamorphosed chondrites in showing no detectable induced TL, which is consistent with literature data that suggests very unusual feldspar in these meteorites. Using TL sensitivity and several mineral systems and allowing for the differences in the oxidized and reduced subgroups, the CV and CV-related meteorites can be divided into petrologic types analogous to those of the ordinary and CO type 3 chondrites. Axtell, Kaba, Leoville, Bali, Arch and ALHA81003 are type 3.0-3.1, while ALH84018, Efremovka, Grosnaja, Allende and Vigarano are type 3.2-3.3 and Coolidge and Loongana 001 are type 3.8. Mokoia is probably a breccia with regions ranging in petrologic type from 3.0 to 3.2. Renazzo often plots at the end of the reduced and oxidized CV chondrite trends, even when those trends diverge, suggesting that in many respects it resembles the unmetamorphosed precursors of the CV chondrites. The low-petrographic types and low-TL peak temperatures of all samples, including the CV3.8 chondrites, indicates metamorphism in the stability field of low feldspar (i.e., less than 800 C) and a metamorphic history similar to that of the CO chondrites but unlike that of the ordinary chondrites.

Guimon, R. Kyle; Symes, Steven J. K.; Sears, Derek W. G.

1995-01-01

117

Factors affecting taste scores of early season seedless table grape cv. Mystery and Prime.  

PubMed

Table grapes of cv. Mystery and Prime were harvested from 10 farms in two growing areas of Israel over two seasons. The grapes were separated on the basis of sucrose solutions from 12 to 18%; soluble solids content (SSC), titratable acidity (TA), and pH were determined; and taste tests were conducted. SSC gave the best correlation with taste tests, and multiple regression of SSC, TA, and pH improved the correlation. There were both seasonal and regional differences in the measured maturity parameters. Lower TA and higher pH were found in grapes from the Jordan Valley. Volatiles were predominantly C(6) compounds hexanal and 2-hexanal, contributing a fresh aroma to the grapes. It is concluded that Mystery and Prime grapes have good organoleptic quality if harvested at SSC levels of >14%. PMID:11804527

Sonego, Lilian; Lurie, Susan; Zuthi, Yohanan; Kaplonov, Tatiana; Ben-Arie, Ruth; Kosto, Itzhak

2002-01-30

118

Effects of agricultural practices on color, carotenoids composition, and minerals contents of sweet peppers, cv. Almuden.  

PubMed

Consumers demand organic products because they believe they are more flavorful and respectful to the environment and human health. The effects of conventional, integrated, and organic farming, grown in a controlled greenhouse, on color, minerals, and carotenoids of sweet pepper fruits ( Capsicum annuum), cv. Almuden, were studied. Experimental results proved that organic farming provided peppers with the highest (a) intensities of red and yellow colors, (b) contents of minerals, and (c) total carotenoids. Integrated fruits presented intermediate values of the quality parameters under study, and conventional fruits were those with the lowest values of minerals, carotenoids, and color intensity. As an example, the concentrations of total carotenoids were 3231, 2493, and 1829 mg kg (-1) for organic, integrated, and conventional sweet peppers, respectively. Finally, organic red peppers could be considered as those having the highest antioxidant activity of all studied peppers (agricultural farming and development stage). PMID:17822289

Pérez-López, Antonio José; López-Nicolas, José Manuel; Núñez-Delicado, Estrella; Del Amor, Francisco M; Carbonell-Barrachina, Angel A

2007-10-01

119

Plant regeneration from protoplasts of embryogenic cell suspensions of Coffea arabica L. cv. caturra.  

PubMed

Coffee plants were regenerated from protoplasts isolated from embryogenic cell suspension cultures derived from somatic embryos of Coffea arabica L. cv. caturra. Yields of viable protoplasts ranged from 1×10(5) to 6×10(5) protoplast/g fresh weight. Protoplast preparations usually contained no contaminating cells, and when present, the number of cells never exceeded 0.1% of the total. Plating efficiencies of protoplast ranged from 1 to 10%. Embryogenic protocolonies obtained after several subcultures in a medium supplemented with 0.5 mg/l each of benzylaminopurine, 2,4-dichlorophenoxyacetic acid and naphtaleneacetic acid, were transferred to a medium lacking plant growth regulators. Well differentiated embryos were formed in selected protocolonies that contained many embryos-like structures. Approximately 70% of the somatic embryos developed into green rooted plantlets which were succesfully transferred to vessels containing sterilized scoria. Plants grown for two months in scoria were finally transferred to greenhouse. PMID:24221671

Acuna, J R; de Pena, M

1991-09-01

120

Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”  

PubMed Central

A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White Cloud Bean”. Both trypsin inhibitors exhibited a molecular mass of 16?kDa and reduced the activity of trypsin with an IC50 value of about 0.6??M. Dithiothreitol attenuated the trypsin inhibitory activity, signifying that an intact disulfide bond is indispensable to the activity. [Methyl-3H] thymidine incorporation by leukemia L1210 cells was inhibited with an IC50 value of 28.8??M and 21.5??M, respectively. They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100??M. PMID:20617140

Sun, Jian; Wang, Hexiang; Ng, Tzi Bun

2010-01-01

121

Screening of a functional polysaccharide from Zizyphus Jujuba cv. Jinsixiaozao and its property.  

PubMed

Chinese jujube has been used as a kind of Chinese medicinal herb for a long time. In this paper, the polysaccharides from Zizyphus Jujuba cv. Jinsixiaozao (ZSP), one of the major Chinese jujube varieties, were extracted and purified sequentially so that its water-soluble immunobiological fractions were screened. The crude ZSP was found to dramatically increase thymus and spleen indices in mice, enhance the proliferation of splenocytes and peritoneal macrophages, and have potential anti-complementary activity. Immunobiological tests indicated that two fractions of ZSP, coded ZSP3c and ZSP4b, were the main active components. ZSP3c was rich in pectin with a degree of esterification (DE) of 49%, which may be related to its stronger immunological activity. PMID:21539856

Li, Jinwei; Shan, Liang; Liu, Yuanfa; Fan, Liuping; Ai, Lianzhong

2011-10-01

122

YEAST dynamics during the natural fermentation process of table olives (Negrinha de Freixo cv.).  

PubMed

Yeast population and dynamics associated to spontaneous fermentation of green table olives Negrinha de Freixo cv. were evaluated. Olives and brine samples were taken at different fermentation times, and yeast were enumerated by standard plate count and identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (rDNA). Saccharomyces cerevisiae was the most frequent, followed by Candida tropicalis, Pichia membranifaciens and Candida boidini, representing together 94.8% of the total isolates. Galactomyces reessii was also identified for the first time in table olives. The highest species diversity was found between 44 and 54 days of fermentation, both in brine and olive pulp. Furthermore, high similarity was observed between brine and olive pulp microbiotas. In conclusion, these results give valuable information to table olive industrials in order to achieve more knowledge on the fermentation process of this important Protected Designation of Origin product. PMID:25475331

Pereira, Ermelinda L; Ramalhosa, Elsa; Borges, Ana; Pereira, José A; Baptista, Paula

2015-04-01

123

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering.  

PubMed

High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B. juncea cv. Geeta. Hypocotyl from 5-day-old seedlings were used as explants. Of the various combinations of auxins and cytokinins tried along with Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium, MS + IAA (0.2 mg/l) + BA (3 mg/l) proved best for shoot regeneration with 89.9 % regeneration efficiency. To induce early flowering Leafy gene from Arabidopsis thaliana was transformed using Agrobacterium mediated transformation method. After 12 weeks transgenic plants showed flowering in vitro whereas their untransformed counterpart did not flower even after 16 weeks. The maximum transformation frequency was 4 %. PMID:24431514

Sahni, Sumit; Ganie, Showkat Hussain; Narula, Alka; Srivastava, Prem Shankar; Singh, Hari Bansh

2013-07-01

124

Overseas trip report, CV 990 underflight mission. [Norwegian Sea, Greenland ice sheet, and Alaska  

NASA Technical Reports Server (NTRS)

The scanning microwave radiometer-7 simulator, the ocean temperature scanner, and an imaging scatterometer/altimeter operating at 14 GHz were carried onboard the NASA CV-990 over open oceans, sea ice, and continental ice sheets to gather surface truth information. Data flights were conducted over the Norwegian Sea to map the ocean polar front south and west of Bear Island and to transect several Nimbus-7 footprints in a rectangular pattern parallel to the northern shoreline of Norway. Additional flights were conducted to obtain correlative data on the cryosphere parameters and characteristics of the Greenland ice sheet, and study the frozen lakes near Barrow. The weather conditions and flight path way points for each of the nineteen flights are presented in tables and maps.

Gloersen, P.; Crawford, J.; Hardis, L.

1980-01-01

125

Partial melting of the Allende (CV3) meteorite - Implications for origins of basaltic meteorites  

NASA Astrophysics Data System (ADS)

Eucrites and angrites are distinct types of basaltic meteorites whose origins are poorly known. Experiments in which samples of the Allende (CV3) carbonaceous chondrite were partially melted indicate that partial melts can resemble either eucrites or angrites, depending only on the oxygen fugacity. Melts are eucritic if this variable is below that of the iron-wuestite buffer or angritic if above it. With changing pressure, the graphite-oxygen redox reaction can produce oxygen fugacities that are above or below those of the iron-wuestite buffer. Therefore, a single, homogeneous, carbonaceous planetoid greater than 110 kilometers in radius could produce melts of drastically different composition, depending on the depth of melting.

Jurewicz, A. J. G.; Mittlefehldt, D. W.; Jones, J. H.

1991-05-01

126

Direct organogenesis from internodal segments of in vitro grown shoots of apple cv. Golden delicious.  

PubMed

A protocol for direct organogenesis from internodal segments of in vitro grown shoots obtained from mature apple cv. Golden delicious trees is presented. Adventitious buds were initiated on Murashige and Skoog medium (1962) containing various combinations of benzylaminopurine (BAP) and 2,3,5-triiodobenzoic acid (TIBA). Low concentration of BAP (4.4 ?M) in combination with TIBA (1 ?M) gave the best percentage of regeneration. Three repeated cycles of culture and regeneration produced an increase of adventitious budding up to 23%. Although no auxin was used in the organogenic medium, callus was always obtained. The regenerated shoots were micropropagated and rooted. Cytological studies revealed that proliferating buds originated directly from the superficial layers of the internodal explants without an intermediate callus phase. PMID:24213782

Belaizi, M; Paul, H; Sangwan, R S; Sangwan-Norreel, B S

1991-01-01

127

Contrasting Size Distributions of Chondrules and Inclusions in Allende CV3  

NASA Technical Reports Server (NTRS)

There are several leading theories on the processes that led to the formation of chondrites, e.g., sorting by mass, by X-winds, turbulent concentration, and by photophoresis. The juxtaposition of refractory inclusions (CAIs) and less refractory chondrules is central to these theories and there is much to be learned from their relative size distributions. There have been a number of studies into size distributions of particles in chondrites but only on relatively small scales primarily for chondrules, and rarely for both Calcium Aluminum-rich Inclusions (CAIs) and chondrules in the same sample. We have implemented macro-scale (25 cm diameter sample) and high-resolution microscale sampling of the Allende CV3 chondrite to create a complete data set of size frequencies for CAIs and chondrules.

Fisher, Kent R.; Tait, Alastair W.; Simon, Jusin I.; Cuzzi, Jeff N.

2014-01-01

128

Agrobacterium-mediated transformation of Solanum tuberosum L. cv. 'Russet Burbank'.  

PubMed

Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency of 60% in the absence of selection and 10% on medium containing 100 mg/l kanamycin monosulfate. Regenerated shoots were assayed for kanamycin resistance by placing stem segments on callus induction medium containing an increased level of kanamycin. Of a total 255 regenerated shoots, 47 (18%) were kanamycin resistant. Of the kanamycin resistant shoots, 25 (53%) expressed the PVX or PVY coat protein genes as assayed by enzyme-linked immunosorbent assay or Western immunoblot analysis. PMID:24226160

Newell, C A; Rozman, R; Hinchee, M A; Lawson, E C; Haley, L; Sanders, P; Kaniewski, W; Tumer, N E; Horsch, R B; Fraley, R T

1991-05-01

129

Relationships between growth stress and wood properties in Poplar1 I-69 (Populus deltoides Bartr. cv."Lux" ex I-69/55)2  

E-print Network

1 Relationships between growth stress and wood properties in Poplar1 I-69 (Populus deltoides Bartr, Annals of Forest Sciences, 65, 30712 13 Abstract - Six inclined poplar I-69 (Populus deltoids cv. I-69 to the growth stress level to be discussed.20 Key-words: populus deltoids cv. I-69/55, poplar, tension wood

Paris-Sud XI, Université de

130

Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan  

PubMed Central

Background We performed an environmental study of viruses infecting the symbiotic single-celled algae of Paramecium bursaria (Paramecium bursaria Chlorella virus, PBCV) in Lake Biwa, the largest lake in Japan. The viruses detected were all Chlorella variabilis virus (CvV = NC64A virus). One of them, designated CvV-BW1, was subjected to further characterization. Results CvV-BW1 formed small plaques and had a linear DNA genome of 370 kb, as judged by pulsed-field gel electrophoresis. Restriction analysis indicated that CvV-BW1 DNA belongs to group H, one of the most resistant groups among CvV DNAs. Based on a phylogenetic tree constructed using the dnapol gene, CvV was classified into two clades, A and B. CvV-BW1 belonged to clade B, in contrast to all previously identified virus strains of group H that belonged to clade A. Conclusions We conclude that CvV-BW1 composes a distinct species within C. variabilis virus. PMID:20831832

2010-01-01

131

Wintercuring of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid in response to Xylella fastidiosa infections  

Technology Transfer Automated Retrieval System (TEKTRAN)

Clonal replicates of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid P 63-61 were inoculated with Xylella fastidiosa strain M23 and evaluated for Almond Leaf Scorch Disease and subsequent wintercuring of infections during three growing seasons. Initial inoculations established gr...

132

A comparison of stilbene and chalcone synthases including a new stilbene synthase gene from Vitis riparia cv. Gloire de Montpellier  

Microsoft Academic Search

A stilbene synthase gene was cloned from Vitis riparia cv. Gloire de Montpellier after PCR amplification with primers designed to include the start and stop codons of stilbene synthase genes of V. vinifera. The exon was very similar to that of other stilbene synthases, particularly those from V. vinifera (99% nucleotide identity). An intron was found which interrupted the predicted

P. H. Goodwin; T. Hsiang; L. Erickson

2000-01-01

133

Phytoremediation of lead with green onions ( Allium fistulosum) and uptake of arsenic compounds by moonlight ferns ( Pteris cretica cv Mayii)  

Microsoft Academic Search

Phytoremediation has been investigated as an alternative to excavation to remediate contamination in soil. In this work, Allium fistulosum (green onions) and Pteris cretica cv Mayii (moonlight ferns) were investigated for phytoremediation. Green onions were planted in lead-spiked soil, and chelating agents were introduced to enhance the uptake of lead by the plants. Lead uptake was low in the absence

Youngsoo Cho; James A. Bolick; David J. Butcher

2009-01-01

134

www.alasdaircohen.com (& www.ifad.org/mpat) Alasdair Cohen, CV (last updated Sept, 2014) 1 Alasdair G. Cohen  

E-print Network

www.alasdaircohen.com (& www.ifad.org/mpat) Alasdair Cohen, CV (last updated Sept, 2014) 1 Alasdair G. Cohen COMPETENCIES M&E/Poverty Metrics | Water & Sanitation | Environmental Health EDUCATION national office & Guangxi Province offices · Managed summer data collection (surveys & drinking water

Silver, Whendee

135

High Efficiency Somatic Embrogenesis and Plant Regeneration in Suspension Cultures of an Ornamental Ginger Hybrid (Hedychium muluense x cv ‘Starburst’)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Plants were successfully regenerated via somatic embryogenesis from shoot apex-derived callus of an ornamental ginger hybrid, Hedychium muluense x cv ‘Starburst’. H. muluense is a dwarf species and ‘Starburst’ is a hybrid cultivar with white and very fragrant flowers in a circular, wheel-like arrang...

136

Inherited transgene expression of the uidA and bar genes in Lilium longiflorum cv. Nellie White  

Technology Transfer Automated Retrieval System (TEKTRAN)

The expression of two transgenes, bar and uidA, was studied in Lilium longiflorum cv. Nellie White plants. ‘Nellie White’ had been transformed using the gene gun to bombard with pDM327 that contains the bar-uidA fusion gene under control of the CaMV 35S promoter. PCR analysis confirmed that eight ...

137

The goal of this project was to examine open CV's face detection software and identify the time insensitive section  

E-print Network

#12;#12;#12;#12;The goal of this project was to examine open CV's face detection software the face detection algorithm by running it hardware instead of software. In order to accomplish this task Altera gave us a Cyclone IV PCI express board. Our final goal being able to start the face detection

Stuart, Josh

138

EFECTO DE LA DENSIDAD DE PLANTACIÓN SOBRE EL CRECIMIENTO VEGETATIVO Y REPRODUCTIVO DE LA FRESA CV. ELSANTA  

Microsoft Academic Search

Effects of the plant density on the vegetative and reproductive growth in strawberry cv. Elsanta The manipulation of plant density is a tool used to optimize the yield in strawberry. However, both the vegetative and reproductive growth are affected by this practice. Furthermore, the effect of plant density depends on the cultivar, agronomic management, and environmental conditions. In this research,

María Pérez de Camacaro; James Carew; Nick Battey

139

Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv. 'Tropic Sun')  

Technology Transfer Automated Retrieval System (TEKTRAN)

Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydr...

140

Internship Checklist for DEB students 1. Before you submit a CV and cover letter to any company, please have Dr.  

E-print Network

Internship Checklist for DEB students Feb 2008 1. Before you submit a CV and cover letter to any an internship (in Objective section) and also list the DEB as part of your PhD education. Example: PhD in Chemistry with a Designated Emphasis in Biotechnology. 3. If you are offered an internship, please notify Dr

Ferrara, Katherine W.

141

The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface*  

PubMed Central

The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is “hijacked” by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288,) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 “self”-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N.; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A.; Bianchet, Mario A.; Wang, Lai-Xi; Wilson, Iain B. H.; Vasta, Gerardo R.

2013-01-01

142

Unusual Dark Clasts in the Vigarano CV3 Chondrite: Record of Parent Body Process  

NASA Astrophysics Data System (ADS)

A variety of dark lithic clasts have been reported from CV3 chondrites and are commonly called "dark inclusions" (DIs). The DIs widely range in texture from chondritic with chondrules and Ca-Al-rich inclusions (CAIs) embedded in a matrix (similar to host meteorites), to fine-grained aggregates of Fe-rich olivine free of coarse-grained components [1,2]. The DIs have been interpreted to represent (1) primary aggregates of materials in the solar nebula [3-5] and (2) materials that were affected by thermal metamorphism on their parent bodies [6]. We present the results of petrographic and scanning electron microscope (SEM) studies of two unusual clasts found in the Vigarano CV3 chondrite. The two unusual clasts, which we will call CL1 and CL2, are approximately 1.2 x 1.0 mm^2 (CLl) and 0.8 x 0.6 mm^2 (CL2) and occur within one of the large clasts (2.8 x 1.0 mm^2). CL1 and CL2 have very similar mineralogies and textures; they contain irregular to oval-shaped inclusions consisting mostly of fine grains of Fe-rich olivine embedded in the matrix of the clasts, and are free of distinct chondrules, CAIs, and coarse mineral fragments. Thus, they resemble the fine-grained variety of DIs. Under the optical microscope, most inclusions resemble chondrules or chondrule fragments in shape and size. However, they are brownish-translucent in transmitted light and are clearly distinct from chondrules in the Vigarano host. The inclusions are characteristically flattened in direction, exhibiting apparent foliation. Our SEM observations reveal the following unusual characteristics: (1) the inclusions are not mere random aggregates of olivine grains but have peculiar internal textures, i.e., assemblies of round or oval-shaped outlines, which are suggestive of pseudomorphs after porphyritic or granular olivine chondrules; (2) one of the thick inclusion rims contains a network of vein-like strings of elongated olivine grains, (3) an Fe-Ni metal aggregate in CL1 has an Fe-, Ni-, S-rich halo, suggesting a reaction between its precursor and the surrounding matrix; and (4) olivine in the clasts commonly shows a swirly, fibrous texture similar to that of phyllosilicate. These characteristics are not reconciled with a primary (unprocessed) origin for the clasts but suggest that they were involved in a secondary process such as aqueous alteration. The fine grains of olivine in these clasts were presumably produced by thermal transformation of phyllosilicate. Serpentine is known to start transforming to olivine at >300 degrees C [7]. The inclusions in CL1 and CL2 resemble chondrules: Some of them are surrounded by distinctive rims similar to chondrule rims commonly seen in the carbonaceous chondrites. Therefore, we believe that the precursor material of the clasts would have been related to some types of chondrites, possibly the Vigarano host itself, and that aqueous alteration and thermal metamorphism occurred in the meteorite parent body. We suggest that some of the dark inclusions and clasts previously reported from CV3 chondrites and other types of meteorites may have origins common to these clasts in Vigarano. References: [1] Fruland R. M. et al. (1978) Proc. LPSC 9th, 1305-1329. [2] Johnson C. A. et al. (1990) GCA, 54, 819-830. [3] Bischoff A. et al. (1988) LPSC XIX, 88-89. [4] Kurat G. (1989) Z. Naturforsch., 44a, 988-1004. [5] Palme H. (1989) Z. Naturforsch., 44a, 1005-1014. [6] Bunch T. E. and Chang S. (1983) LPSC XIV, 75-76. [7] Akai J. (1992) Proc. NIPR Symp. Antarct. Meteorites, 5, 120-135.

Kojima, T.; Tomeoka, K.; Takeda, H.

1993-07-01

143

Characterization of Fusarium wilt resistant somaclonal variants of banana cv. Rasthali by cDNA-RAPD.  

PubMed

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is counted among the most destructive diseases of crop plants in India. In the absence of any credible control measure to manage this disease, development of resistant cultivars is the best option. Somaclonal variations arising out of long term in vitro culture of plant tissues is an important source of genetic variability and the selection of somaclones having desired characteristics is a promising strategy to develop plants with improved characters. In the present study, we isolated a group of somaclonal variants of banana cv. Rasthali which showed efficient resistance towards Foc race 1 infection in repeated bioassays. cDNA-RAPD methodology using 96 decamer primers was used to characterize these somaclonal variants. Among the four differentially amplified bands obtained, one mapping to the coding region of a lipoxygenase gene was confirmed to be down regulated in the somaclones as compared to controls by real-time quantitative RT-PCR. Our results correlated well with earlier studies with lipoxygenase mutants in maize wherein reduced expression of lipoxygenase led to enhanced resistance towards Fusarium infection. PMID:25160909

Ghag, Siddhesh B; Shekhawat, Upendra K S; Ganapathi, Thumballi R

2014-12-01

144

About the determination of the Schottky barrier height with the C-V method  

NASA Astrophysics Data System (ADS)

On the basis of general notions about Schottky barrier contacts (SBC) with the insulating layer and interface states (ISs) communicating with semiconductor and metal (when their influence results in the linear bias-dependence of the barrier height and the ideality factor n =const), it is shown that the barrier height determined with C-V method is defined with a simple expression: ?bc=n?b0-(n -1)(?s+V2), practically corresponding to the flatband barrier height expression determined from I-V-characteristic: ?bf=n?b0-(n -1)?s. The apparent difference is related to the difference in implementation of the flatband condition in both cases. Earlier, the close correspondence of values ?bc and ?bf was only known for the ideal Bardeen model, practically excluding values n >1. The received result is also proved by the detailed analysis of possible conditions of determining the SBC capacity (different frequencies of the test signal, presence or absence of ISs, and their communication with semiconductor and/or metal). It is essential that the measured barrier height ?bc remains almost independent of the frequency of the test signal and is determined with the relation between the applied voltage and its part dropping in the barrier only.

Bozhkov, V. G.; Torkhov, N. A.; Shmargunov, A. V.

2011-04-01

145

Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)  

PubMed Central

A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 ?M, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 ?M. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172

Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng

2009-01-01

146

Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. Cv. MRQ 74)  

PubMed Central

Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5?mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5?mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1?mg/L BAP, and 0.1?mg/L ?-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1?mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds. PMID:22919338

Taha, Rosna Mat; Saleh, Azani; Mahmad, Noraini; Hasbullah, Nor Azlina; Mohajer, Sadegh

2012-01-01

147

Effect of sowing dates and vernalization on Betavulgaris L. cv. Univers C-leaf structure  

PubMed Central

This research was conducted to study the effect of three different sowing dates (15th October, 15th November and 15th December) and two vernalization treatments (5 °C and ?20 °C) on leaf structure of Betavulgaris L. cv. Univers. The obtained data are summarized as follows: The maximum values of the most studied parameters; lower epidermis + spongy tissue thickness, midrib, mesophyll tissue, vascular bundle, collenchymatous tissue and number of xylem vessels per arm were found as a result of 15th October sowing date treatment compared with the two other sowing dates. Furthermore, effect of the cooling treatments varied according to the recorded character, sowing date and cooling degree. Most of the vernalization treatments at early sowing dates increased the mesophyll tissue, midrib, number of vascular bundles per transverse section, vascular bundle thickness and number of xylem arms per transverse section. The two studied cooling treatments at 15th October sowing date increased both stomatal index and average number of stomata: average number of epidermis cells compared with the control. Furthermore, 15th October under ?20 °C treatment led to small epidermal cells and stomata formation, straight epidermal cell walls and closed stomata in comparison to the control. PMID:23961134

Sakr, Mohammed M.; Almaghrabi, Omar A.

2011-01-01

148

The impact of high temperatures on Vitis vinifera cv. Semillon grapevine performance and berry ripening.  

PubMed

The heat event that occurred in many parts of Australia in 2009 was the worst on record for the past decade, with air temperatures exceeding 40(°)C for 14 days. Our aim was to assess the impacts of this heat event on vine performance, including ripening, yield, and gas exchange of Vitis vinifera cv. Semillon grown in a Riverina vineyard. To assess the affect of high temperatures on Semillon grapevines, the vines were covered with a protective layer to reduce radiant heating and were compared with vines exposed to ambient conditions. The heat event had major effects on ripening; reducing the rate of ripening by 50% and delaying harvest ripeness and causing a high incidence of berry shrivel and sunburn. Yield was not affected. Photosynthesis was reduced 35% by the heat event while transpiration increased nearly threefold and was accounted for by increased stomatal conductance. The conclusion of this study was that heat events delayed ripening in Semillon berries and caused a significant reduction in berry quality. Strategies to minimize the radiant load during heat events are required and this study has confirmed a protective layer can reduce canopy temperatures and enhance berry quality. PMID:24348494

Greer, Dennis H; Weedon, Mark M

2013-01-01

149

The impact of high temperatures on Vitis vinifera cv. Semillon grapevine performance and berry ripening  

PubMed Central

The heat event that occurred in many parts of Australia in 2009 was the worst on record for the past decade, with air temperatures exceeding 40°C for 14 days. Our aim was to assess the impacts of this heat event on vine performance, including ripening, yield, and gas exchange of Vitis vinifera cv. Semillon grown in a Riverina vineyard. To assess the affect of high temperatures on Semillon grapevines, the vines were covered with a protective layer to reduce radiant heating and were compared with vines exposed to ambient conditions. The heat event had major effects on ripening; reducing the rate of ripening by 50% and delaying harvest ripeness and causing a high incidence of berry shrivel and sunburn. Yield was not affected. Photosynthesis was reduced 35% by the heat event while transpiration increased nearly threefold and was accounted for by increased stomatal conductance. The conclusion of this study was that heat events delayed ripening in Semillon berries and caused a significant reduction in berry quality. Strategies to minimize the radiant load during heat events are required and this study has confirmed a protective layer can reduce canopy temperatures and enhance berry quality. PMID:24348494

Greer, Dennis H.; Weedon, Mark M.

2013-01-01

150

Effect of chemical pretreatment on dehulling parameters of flaxseed (cv. Garima).  

PubMed

In the present study, effects of chemical (ethanol, HCl and sulphuric acid) pretreatment on various dehulling parameters of flaxseed (cv. Garima) including yield, hull, hullability, extraction rate and embryo (dehulled flaxseed) recovery were studied. Pretreated flaxseed, at 3.1 to 3.6 % moisture range (p?>?0.05) were dehulled for 60 s in a laboratory model rice polisher/dehulling machine at 2,000 rpm followed by aspiration (hull separation) using a laboratory model aspirator. The study revealed that chemical pre-dehulling treatment of flaxseed plays a significant role in the embryo recovery of flaxseed. Both ethanol and HCl pre-dehulling treatment enhanced but sulphuric acid pretreatment reduced the embryo recovery of flaxseed. Moreover, HCL and Sulphuric acid deteriorated the quality of hull obtained during dehulling, hence may not be considered for flaxseed dehulling. The study showed the maximum embryo recovery from ethanol pretreated flaxseed, hence ethanol pre-dehulling treatment with 2 h tempering time may be considered for effective flaxseed dehulling. PMID:25190888

Mridula, D; Barnwal, P; Gurumayum, Sushma; Singh, K K

2014-09-01

151

CV-990 Landing Systems Research Aircraft (LSRA) flight #145 drilling of shuttle tire using Tire Assa  

NASA Technical Reports Server (NTRS)

Created from a 1/16th model of a German World War II tank, the TAV (Tire Assault Vehicle) was an important safety feature for the Convair 990 Landing System Research Aircraft, which tested space shuttle tires. It was imperative to know the extreme conditions the shuttle tires could tolerate at landing without putting the shuttle and its crew at risk. In addition, the CV990 was able to land repeatedly to test the tires. The TAV was built from a kit and modified into a radio controlled, video-equipped machine to drill holes in aircraft test tires that were in imminent danger of exploding because of one or more conditions: high air pressure, high temperatures, and cord wear. An exploding test tire releases energy equivalent to two and one-half sticks of dynamite and can cause severe injuries to anyone within 50 ft. of the explosion, as well as ear injury - possibly permanent hearing loss - to anyone within 100 ft. The degree of danger is also determined by the temperature pressure and cord wear of a test tire. The TAV was developed by David Carrott, a PRC employee under contract to NASA.

1995-01-01

152

Growth and Respiratory Response of Fig (Ficus carica L. cv. Mission) Fruits to Ethylene.  

PubMed

Growth in diameter of the fig (Ficus carica L. cv. Mission) fruit takes place in three distinct periods; two periods (I and III) of rapid growth are separated by a period (II) of slow growth. With respect to exposure to ethylene, the fruit exhibits a two phase response. Ethylene inhibits fruit growth in phase A (period I), the period of cell division, stimulates growth in early phase B (early period II), and stimulates both growth and ripening during the remainder of phase B (late period II and period III). The adverse effect of exogenous ethylene on fruits during phase A is thought to be due to inhibition of cell division. The gradual transition occurring in the response of fruits during phase B was interpreted in terms of carbohydrate level in the fruits.The onset of period III and a respiratory climacteric rise was preceded by or concomitant with a sudden burst of endogenous ethylene synthesis. This, together with the fact that exogenous ethylene applied at the proper stage of fruit growth triggers both ripening and the climacteric rise, leads to the conclusion that ethylene is the causal agent. In other words, the data support the concept that ethylene is a growth hormone that initiates a chain of metabolic and physiological events leading to fig fruit ripening. PMID:16657774

Marei, N; Crane, J C

1971-09-01

153

Purification and characterization of a lectin from Phaseolus vulgaris cv. (Anasazi beans).  

PubMed

A lectin has been isolated from seeds of the Phaseolus vulgaris cv. "Anasazi beans" using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1-14 and the temperature range of 0-80 degrees C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC(50) of 1.3 microM, and inhibited the activity of HIV-1 reverse transcriptase with an IC(50) of 7.6 microM. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172

Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng

2009-01-01

154

Acid and Neutral Invertases in the Mesocarp of Developing Muskmelon (Cucumis melo L. cv Prince) Fruit  

PubMed Central

Acid and neutral invertases were found in the mesocarp of developing muskmelon (Cucumis melo L. cv Prince) fruit and the activities of these enzymes declined with maturation of the fruit, concomitantly with the accumulation of sucrose. Neutral invertase was only present in the soluble fraction and acid invertase was present in both the soluble and cell-wall fractions. The cell-wall fraction contained three types of acid invertase: a NaCl-released invertase; an EDTA-released invertase, and a tightly bound invertase that still remained on the cell wall after treatment with NaCl and EDTA. The soluble acid and neutral invertases could be separated from one another by chromatography on DEAE-cellulose and they exhibited clear differences in their properties, namely, in their pH optima, substrate specificity, Km values for sucrose, and inhibition by metal ions. The EDTA-released invertase and the soluble acid invertase were similar with regard to their chromatographic behavior on DEAE-cellulose, but the NaCl-released invertase was different because it was adsorbed to a column of CM-cellulose. The soluble acid invertase and two cell-wall bound invertases had very similar characteristics with regard to optimal pH and temperature, Km value for sucrose, and substrate specificity. PMID:16668269

Ranwala, Anil P.; Iwanami, Shun-Suke; Masuda, Hiroshi

1991-01-01

155

In vitro regeneration in olive (Olea europaea L.) cv, 'Frontio' from nodal segments.  

PubMed

An efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv 'Frontio' has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurine (BAP), which resulted in 56.2% of bud break and 93.7% survival whereas, a combination of full strength MS medium with 1.0 mg/L each of 3-indole-butyric-acid (IBA) and kinetin was found to be the best for shoot multiplication, in terms of number of shoots (3.6 shoots/explant) and shoot length (2.2 cm). The in vitro shoots were rooted on half-strength MS medium fortified with 0.2 mg/L IBA and 0.2 mg/L alpha-naphthalene acetic acid (NAA) with 1.5 g/L activated charcoal, which supported optimum rooting (60%), with an average of 2-3 roots/shoot, about 2.4 cm length were produced on four weeks of culture. PMID:25241592

Mangal, Manisha; Sharma, Dheeraj; Sharma, Mamta; Kumar, Sunil

2014-09-01

156

Folate levels and polyglutamylation profiles of papaya (Carica papaya cv. Maradol) during fruit development and ripening.  

PubMed

Folates are essential micronutrients for humans, and their deficiency causes several detrimental effects on human health. Papaya fruit is an important natural source of some micronutrients. This paper presents a first complete characterization of folate derivatives accumulated in cv. Maradol papaya during fruit development and ripening processes. During postharvest ripening, the fruit accumulated up to 24.5% of the daily folate recommended dietary allowance (RDA) for an adult in a 1 cup (145 g) portion. Tetrahydrofolate (THF) and 5-methyl-THF were the predominant folate classes observed. Surprisingly, an unusually long polyglutamylation profile of tentatively up to 17 glutamates linked to 5-methyl-THF was detected; to the authors' knowledge, this very long polyglutamyl tail has not been reported for any organism, and it is probably characteristic of this plant species. This polyglutamylation degree changed throughout fruit development and ripening, showing the largest differences at the onset of ripening. This work raises questions about the functional role of folate derivatives in fruit development. PMID:23574547

Ramos-Parra, Perla A; García-Salinas, Carolina; Hernández-Brenes, Carmen; de la Garza, Rocío I Díaz

2013-04-24

157

Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.  

PubMed

Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to approximately 36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants. PMID:16604461

Kanamoto, Hirosuke; Yamashita, Atsushi; Asao, Hiroshi; Okumura, Satoru; Takase, Hisabumi; Hattori, Masahira; Yokota, Akiho; Tomizawa, Ken-Ichi

2006-04-01

158

Specific poly-phenolic compounds in cell culture of Vitis vinifera L. cv. Gamay Fréaux.  

PubMed

Cell cultures established from plants represent an attractive alternative to whole plants for effective production of bioactive secondary metabolites. Cell culture from Vitis vinifera L. cv. Gamay Fréaux accumulated high amounts of hydroxycinnamic acid derivatives and anthocyanins. Two new compounds were identified: 3-O-glucosylresveratrol, a stilbene derivative, abundant in cell suspension culture, and a hydroxyphenol, 4-(3,5-dihydroxyphenyl)-phenol, abundant in callus culture. The major anthocyanin monoglucosides present in cell suspension culture were cyanidin 3-O-glucoside and peonidin 3-O-glucoside, and the major cinnamoyl derivatives were cyanidin 3-O-p-coumaryl glucoside and peonidin 3-O-p-coumaryl glucoside. Three minor anthocyanin compounds were found in V. vinifera cell culture: delphinidin 3-O-glucoside, petunidin 3-O-glucoside, and delphinidin 3-O-pcoumaryl glucoside. Anthocyanin levels of cell suspension cultures increased significantly--about eight fold--after 4-day cultivation in new medium. Salicylic acid at a concentration of 50 ?M did not enhance anthocyanin accumulation in cell suspension culture, and similar levels of jasmonic acid significantly reduced the anthocyanin content. PMID:21061087

Mewis, Inga; Smetanska, Iryna M; Müller, Carsten T; Ulrichs, Christian

2011-05-01

159

Molecular properties and activities of tuber proteins from starch potato cv. Kuras.  

PubMed

Potato starch production leaves behind a huge amount of juice. This juice is rich in protein, which might be exploited for food, biotechnological, and pharmaceutical applications. In northern Europe cv. Kuras is dominant for industrial starch production, and juice protein of freshly harvested mature tubers was fractionated by Superdex 200 gel filtration. The fractions were subjected to selected activity assays (patatin, peroxidase, glyoxalases I and II, alpha-mannosidase, inhibition of trypsin, Fusarium protease, and alcalase) and protein subunit size determination by SDS-PAGE and mass spectrometry. Proteins present in SDS-PAGE bands were identified by tryptic peptide mass fingerprinting. Protein complexes such as ribosomes and proteasomes eluted with the void volume of the gel filtration. Large proteins were enzymes of starch synthesis dominated by starch phosphorylase L-1 (ca. 4% of total protein). Five identified dimeric patatin variants (25%) coeluted with four monomeric lipoxygenase variants (10%) at 97 kDa. Protease inhibitor I variants (4%) at 46 kDa (hexamer) inhibited alcalase. Fourteen Kunitz protease inhibitor variants (30%) at 19 kDa inhibited trypsin and Fusarium protease. Carboxypeptidase inhibitor variants (5%) and defensins (5%) coeluted with phenolics. The native sizes and molecular properties were determined for 43 different potato tuber proteins, several for the first time. PMID:17147423

Jørgensen, Malene; Bauw, Guy; Welinder, Karen G

2006-12-13

160

Transpiration, crop coefficient and water use of Olive tree (cv. Cordovil) in Southern Portugal  

NASA Astrophysics Data System (ADS)

Orchard olive transpiration, soil water status and stomatal response in relation to water deficit were investigated to clarify mechanisms of tree water uptake and stomatal control to improve the irrigation scheduling of low-density olive trees of cv. Cordovil grown in typical Mediterranean environment of Southern Portugal. Trees were subject to three irrigation treatments. Treatment A received 100% of crop evapotranspiration by a drip irrigation system, a sustained deficit (SDI)treatment B received 60% of crop evapotranspiration, a regulated deficit(RDI) irrigation treatment C received irrigation water before-flowering and just before pit-hardening, and a Dry-farming treatment. Tree and orchard transpiration and the dynamics of water uptake by roots were estimated from sap flow measurements and water balance technique. Stomatal conductance was modeled from local meteorological variables, measured sap flow and tree canopy variables. Higher than treatment A and B stomatal conductance and the high tree fruit production recommend treatment C as most suitable for scheduling irrigation of olive orchards in wet years of well distributed late summer rainfall. For drier years of no summer and early autumn rains that minimizes available water to extract by roots outside the wet bulb of drip irrigation and for the scarce readily available irrigation water years, as so often occurs in the region, the sustained deficit irrigation (SDI) regime seems a better option. Nonetheless, for years of limited available water resources that preclude sustained deficit irrigation, careful management of the proposed RDI could also allow for efficient use of irrigation water.

Santos, F. L.

2008-12-01

161

Occurrence of metaxenia and false hybrids in Brassica juncea L. cv. Kikarashina × B. napus  

PubMed Central

Imported genetically modified (GM) canola (Brassica napus) is approved by Japanese law. Some GM canola varieties have been found around importation sites, and there is public concern that these may have any harmful effects on related species such as reduction of wild relatives. Because B. juncea is distributed throughout Japan and is known to be high crossability with B. napus, it is assumed to be a recipient of B. napus. However, there are few reports for introgression of cross-combination in B. juncea × B. napus. To assess crossability, we artificially pollinated B. juncea with B. napus. After harvesting a large number of progeny seeds, we observed false hybrids and metaxenia of seed coats. Seed coat color was classified into four categories and false hybrids were confirmed by morphological characteristics and random amplified polymorphic DNA (RAPD) markers. Furthermore, the occurrence of false hybrids was affected by varietal differences in B. napus, whereas that of metaxenia was related to hybridity. Therefore, we suggest that metaxenia can be used as a marker for hybrid identification in B. juncea L. cv. Kikarashina × B. napus. Our results suggest that hybrid productivity in B. juncea × B. napus should not be evaluated by only seed productivity, crossability ought to be assessed the detection of true hybrids. PMID:23136472

Tsuda, Mai; Konagaya, Ken-ichi; Okuzaki, Ayako; Kaneko, Yukio; Tabei, Yutaka

2011-01-01

162

Chlorophyll Biosynthetic Reactions during Senescence of Excised Barley (Hordeum vulgare L. cv IB 65) Leaves.  

PubMed Central

The chlorophyll (Chl) biosynthetic reactions were monitored during senescence of dark-incubated excised barley (Hordeum vulgare L. cv IB 65) leaves floated in double-distilled water or kinetin solution. Kinetin abolished the degradation of Chl but failed to check the net degradation of protochlorophyllide (Pchlide), suggesting that different sets of enzymes, i.e. kinetin sensitive and insensitive, are responsible for the degradation of Chl and Pchlide, respectively. Upon exposure of the leaves to light, the dark-accumulated Pchlide was efficiently phototransformed to chorophyllide (Chlide), even on the 7th d of dark incubation, demonstrating that the activity of Pchlide reductase, one of the late enzymes of the Chl biosynthetic pathway, is not substantially affected during senescence. The senescing leaves continued to synthesize Pchlide and Chlide until the 7th d, although at a reduced rate (20% of the 1st d). The decline of the rate of synthesis of Pchlide and Chlide is due to the loss of activity of two early enzymes of the Chl biosynthetic pathway, i.e. 5-aminolevulinic acid dehydratase and porphobilinogen deaminase. Kinetin substantially checked the loss of activity of these two enzymes. PMID:12232286

Hukmani, P.; Tripathy, B. C.

1994-01-01

163

The effects of Pierce's disease on leaf and petiole hydraulic conductance in Vitis vinifera cv. Chardonnay.  

PubMed

In this study, we test the hypothesis that the symptoms of Pierce's Disease (PD) result from the occlusion of xylem conduits by the bacteria Xylella fastidiosa (Xf ). Four treatments were imposed on greenhouse-grown Vitis vinifera cv. Chardonnay: well-watered and deficit-irrigated plants with and without petiole inoculation with Xf. The hydraulic conductance of the stem-petiole junction (k(jun)) and leaves (k(leaf)) were measured, and Xf concentrations were established by quantitative polymerase chain reaction (qPCR). Leaf hydraulic conductance decreased with increasing leaf scorch symptoms in both irrigation treatments. The positive relationship between Xf concentration and symptom formation in deficit-irrigated plants suggests that water-stress increases susceptibility to PD. In field-grown vines, water relations of symptomatic leaves were similar to naturally senescing leaves but differed from green control leaves. Overall, these results suggest that the development of PD symptoms represents a form of accelerated senescence as part of a systemic response of the plant to Xf infection. PMID:19470095

Choat, Brendan; Gambetta, Greg A; Wada, Hiroshi; Shackel, Kenneth A; Matthews, Mark A

2009-08-01

164

The BANANA project. V. Misaligned and precessing stellar rotation axes in CV Velorum  

E-print Network

As part of the BANANA project (Binaries Are Not Always Neatly Aligned), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of $\\beta_{\\rm p} = -52\\pm6^{\\circ}$ and $\\beta_{\\rm s}= 3\\pm7^{\\circ}$ for the primary and secondary stars (B2.5V $+$ B2.5V, $P=6.9$ d). We combine this information with several measurements of changing projected stellar rotation speeds ($v \\sin i_{\\star}$) over the last $30$ years, leading to a model in which the primary star's obliquity is $\\approx65^{\\circ}$, and its spin axis precesses around the total angular momentum vector with a period of about $140$ years. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the $v \\sin i_{\\star}$. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronge...

Albrecht, Simon; Torres, Guillermo; Fabrycky, Daniel C; Setiawan, Johny; Gillon, Michael; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

2014-01-01

165

The BANANA Project. V. Misaligned and Precessing Stellar Rotation Axes in CV Velorum  

NASA Astrophysics Data System (ADS)

As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of ?p = -52° ± 6° and ?s = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i sstarf) over the last 30 yr, leading to a model in which the primary star's obliquity is ?65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i sstarf. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary. Based on observations made with ESOs 2.2 m Telescopes at the La Silla Paranal Observatory under programme ID 084.C-1008 and under MPIA guaranteed time.

Albrecht, Simon; Winn, Joshua N.; Torres, Guillermo; Fabrycky, Daniel C.; Setiawan, Johny; Gillon, Michaël; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

2014-04-01

166

Noble gases in individual chondrules of the Allende CV3 chondrite  

NASA Astrophysics Data System (ADS)

We analyzed noble gases in nine individual chondrules, an assemblage of small chondrules, and four whole-rock samples of the Allende CV3 chondrite. Major elements were also determined for five chondrules. The cosmic ray exposure ages are calculated from cosmogenic 3He to be 5.17 ± 0.38 and 5.15 ± 0.25 Myr for the averages of the chondrules and whole rocks, respectively, showing no significant pre-exposure evidence for the studied chondrules. Large amounts of 36Ar, 80,82Kr, and 128Xe produced by neutron capture are observed in most samples; the abundances of these nuclides are correlated among the samples. The epithermal neutron flux and neutron slowing down density are calculated based on [80Kr]n, from which a sample depth of about 30 cm can be calculated. The measured chondrules contain variable amounts of radiogenic 129Xe. The abundance ratios of radiogenic 129Xe to neutron capture-produced 128Xe are rather constant among the studied chondrules; four chondrules give more precise ratios at the high-temperature fractions, ranging from 1920 ± 80 to 2280 ± 140, which corresponds to a time difference of 3.9 ± 2.4 Myr. It is noticeable that most chondrules also contain 244Pu-derived fission Xe. The average 244Pu/238U ratio for nine chondrules is 0.0069 ± 0.0018, which agrees well with the preferred ratio reported for chondrites.

Miura, Yayoi N.; Nagao, Keisuke; Kimura, Makoto

2014-06-01

167

Diurnal cycles of embolism formation and repair in petioles of grapevine (Vitis vinifera cv. Chasselas)  

PubMed Central

The impact of water deficit on stomatal conductance (gs), petiole hydraulic conductance (Kpetiole), and vulnerability to cavitation (PLC, percentage loss of hydraulic conductivity) in leaf petioles has been observed on field-grown vines (Vitis vinifera L. cv. Chasselas). Petioles were highly vulnerable to cavitation, with a 50% loss of hydraulic conductivity at a stem xylem water potential (?x) of –0.95?MPa, and up to 90% loss of conductivity at a ?x of –1.5?MPa. Kpetiole described a daily cycle, decreasing during the day as water stress and evapotranspiration increased, then rising again in the early evening up to the previous morning's Kpetiole levels. In water-stressed vines, PLC increased sharply during the daytime and reached maximum values (70–90%) in the middle of the afternoon. Embolism repair occurred in petioles from the end of the day through the night. Indeed, PLC decreased in darkness in water-stressed vines. PLC variation in irrigated plants showed the same tendency, but with a smaller amplitude. The Chasselas cultivar appears to develop hydraulic segmentation, in which petiole cavitation plays an important role as a ‘hydraulic fuse’, thereby limiting leaf transpiration and the propagation of embolism and preserving the integrity of other organs (shoots and roots) during water stress. In the present study, progressive stomatal closure responded to a decrease in Kpetiole and an increase in cavitation events. Almost total closure of stomata (90%) was measured when PLC in petioles reached >90%. PMID:21447755

Zufferey, V.; Cochard, H.; Ameglio, T.; Spring, J.-L.; Viret, O.

2011-01-01

168

Xanthine Oxidase Inhibitor Activity of Terpenoid and Pyrrole Compounds Isolated from Snake Fruit (Salacca edulis Reinw.) cv. Bongkok  

Microsoft Academic Search

The compound of 3β-hydroxy-sitosterol (1) and 2-metylester-1-H-pyrrole-4-carboxilyc acid were isolated from ethyl acetate extract of snake fruit (Salacca edulis Reinw) cv. Bongkok, (2). Inhibition of xanthine oxidase by the two compounds were evaluated against enzyme of xanthine oxidase. Compound 1 could be regarded as inactive, while compound 2 was found to be active with IC50 value of 48.86 μg mL-1.

Leni Herliani Afrianti Priyatno; Elin Yulinah Sukandar; Slamet Ibrahim; I. Ketut Adnyana

2007-01-01

169

Direct shoot organogenesis on hypocotyl explants from in vitro germinated seedlings of Psidium guajava L. cv. Allahabad Safeda  

Microsoft Academic Search

Multiple shoots were generated via direct organogenesis on hypocotyl segments excised from in vitro germinated seedlings (45-day-old) of Psidium guajava L. cv. Allahabad Safeda. Modified basal Murashige and Skoog (MMS) medium supplemented with 6-benzylaminopurine (BAP), zeatin or thidiazuron with or without ?-naphthalene acetic acid (NAA) were tried. Thidiazuron (0.1?M) along with 0.54?M NAA gave the highest response (44.6%) with the

S. K Singh; P. R Meghwal; H. C Sharma; S. P Singh

2002-01-01

170

An interspecific somatic hybrid between upland cotton ( G. hirsutum L. cv. ZDM-3) and wild diploid cotton ( G. klotzschianum A.)  

Microsoft Academic Search

Somatic hybrids were produced through protoplast electrofusion between the tetraploid cotton Gossypium hirsutum L. cv. ZDM-3 and the wild diploid cotton G. klotzschianum. Hybrid plants were generated from 3 out of 24 callus lines that were derived from fused protoplasts. Hybrid plants were\\u000a initially identified as somatic hybrids by ploidy analysis: the plants from the 3 callus lines had chromosome

Yuqiang SunShoumei; Shoumei Liu; Yu Wang; Brian Joseph Jones; Huizhong Wang; Shuijin Zhu

2011-01-01

171

OpenCV C# wrapper based video enhancement using different optical flow methods in the super-resolution  

Microsoft Academic Search

This article presents a simple method on how to implement a super-resolution based video enhancement technique in .NET using the functions of the OpenCV library. First, we outline the goal of this project and after that, a short review of the steps of super-resolution technique is given. As a part of the discussion about the concrete implementation, firstly the general

A. Nagy; Z. Vamossy

2008-01-01

172

Effect of the rootstock on the occurrence of lime-induced chlorosis of potted Vitis vinifera L. cv. ‘Pinot blanc’  

Microsoft Academic Search

The chlorosis susceptible Vitis vinifera L. cv. ‘Pinot blanc’ was grafted on two hybrid rootstocks with different iron efficiency, as follows: V. Berlandieri × V. rupestris ‘140 Ru’ (iron-efficient) and V. riparia × V. rupestris ‘101-14’ (iron-inefficient). The grafted vines were grown in pots of a calcareous and a non-calcareous soil. The shoot growth was periodically checked and leaves, selected

L. Bavaresco; P. Fraschini; A. Perino

1993-01-01

173

The influence of storage on aroma, soluble solids, acid and colour of sour cherries (Prunus cerasus L.) cv. Stevnsbær  

Microsoft Academic Search

The present study involved a laboratory scale experiment where the impact of post-harvest storage on the quality of sour\\u000a cherries (Prunus cerasus L.) cv Stevnsb?r was investigated. Cherries were stored for 7 days at temperatures of 2???C, 10???C, 20???C, and 30???C,\\u000a and at 20???C in combination with a 20% CO2 atmosphere. Cherry quality was assessed by analysis of soluble solids,

M. B. Petersen; L. Poll

1999-01-01

174

Improved Antioxidant and Anti-inflammatory Potential in Mice Consuming Sour Cherry Juice ( Prunus Cerasus cv. Maraska)  

Microsoft Academic Search

The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and\\u000a anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and\\u000a brain), LPO (liver, brain)

Ana Šari?; Sandra Sobo?anec; Tihomir Balog; Borka Kuši?; Višnja Šverko; Verica Dragovi?-Uzelac; Branka Levaj; Zrinka ?osi?; Željka Ma?ak Šafranko; Tatjana Marotti

2009-01-01

175

CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Terra-FM2_Edition1-CV)  

NASA Technical Reports Server (NTRS)

Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

176

Plant Regeneration from Immature Inflorescence Culture and Genetic Transformation of Wheatgrass ( Agropyron cristatum × A. desertorum cv. Hycrest-Mengnong)  

Microsoft Academic Search

The plants of hybrid wheatgrass (A. cristatum × A. desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on improved MS medium containing 2.0-3.0 mg L?1 2,4-D to regenerate callus. The calli were then transferred to hormone-free MS medium for differentiation and 1\\/2 MS medium for rooting. Results showed that callus initiation

Xiu-wen HUO; Jian-hua WEI; Chun-bo XU; Fu-gui MI; Jin-feng YUN

2006-01-01

177

Phosphatidylinositol 4,5-Bisphosphate Induces Actin Stress-Fiber Formation and Inhibits Membrane Ruffling in Cv1 Cells  

Microsoft Academic Search

Phosphatidylinositol 4,5 bisphosphate (PIP 2 ) is widely implicated in cytoskeleton regulation, but the mechanisms by which PIP 2 effect cytoskeletal changes are not defined. We used recombinant adenovirus to in- fect CV1 cells with the mouse type I phosphatidylinosi- tol phosphate 5-kinase a (PIP5KI), and identified the players that modulate the cytoskeleton in response to PIP 2 signaling. PIP5KI

Masaya Yamamoto; Donald H. Hilgemann; Siyi Feng; Haruhiko Bito; Hisamitsu Ishihara; Yoshikazu Shibasaki; Helen L. Yin

2001-01-01

178

Anthocyanin compositions and biological activities from the red petals of Korean edible rose ( Rosa hybrida cv. Noblered)  

Microsoft Academic Search

The objectives of the present study were to investigate anthocyanin profiles and their biological properties, including antioxidant, anticancer, and antiallergic, from the red petals of Korean edible rose (Rosa hybrida cv. Noblered). The acidic methanol extract of this species showed potent biological activities at a concentration of 50?g\\/mL. Its anthocyanins were characterised as cyanidin 3,5-di-O-glucoside and pelargonidin 3,5-di-O-glucoside using reversed-phase

Jin Hwan Lee; Hyeon-Jin Lee; Myoung-Gun Choung

2011-01-01

179

CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Terra-FM1_Edition1-CV)  

NASA Technical Reports Server (NTRS)

Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

180

CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Aqua-FM3_Edition1-CV)  

NASA Technical Reports Server (NTRS)

Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-11-02] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

181

CERES BiDirectional Scans (BDS) data in HDF (CER_BDS_Aqua-FM4_Edition1-CV)  

NASA Technical Reports Server (NTRS)

Each BiDirectional Scans (BDS) data product contains twenty-four hours of Level-1b data for each CERES scanner instrument mounted on each spacecraft. The BDS includes samples taken in normal and short Earth scan elevation profiles in both fixed and rotating azimuth scan modes (including space, internal calibration, and solar calibration views). The BDS contains Level-0 raw (unconverted) science and instrument data as well as the geolocated converted science and instrument data. The BDS contains additional data not found in the Level-0 input file, including converted satellite position and velocity data, celestial data, converted digital status data, and parameters used in the radiance count conversion equations. The following CERES BDS data sets are currently available: CER_BDS_TRMM-PFM_Edition1 CER_BDS_Terra-FM1_Edition1 CER_BDS_Terra-FM2_Edition1 CER_BDS_Terra-FM1_Edition2 CER_BDS_Terra-FM2_Edition2 CER_BDS_Aqua-FM3_Edition1 CER_BDS_Aqua-FM4_Edition1 CER_BDS_Aqua-FM3_Edition2 CER_BDS_Aqua-FM4_Edition2 CER_BDS_Aqua-FM3_Edition1-CV CER_BDS_Aqua-FM4_Edition1-CV CER_BDS_Terra-FM1_Edition1-CV CER_BDS_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2005-03-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

182

Adenoviruses 16 and CV23 Efficiently Transduce Human Low-passage Brain Tumor and Cancer Stem Cells  

Microsoft Academic Search

Most clinical protocols involving adenovirus (Ad) vectors for gene therapy use a vector based on serotype 5 (Ad5). We believe that this serotype is not suitable for all gene therapy applications and that alternative vectors based on other serotypes should be developed. We have compared the ability of Ad5, Ad11p, Ad16p, and a chimpanzee Ad (CV23) to infect human low-passage

Johan Skog; Karin Edlund; A Tommy Bergenheim; Göran Wadell

2007-01-01

183

Characterization of bisphenol A metabolites produced by Portulaca oleracea cv. by liquid chromatography coupled with tandem mass spectrometry.  

PubMed

The garden plant portulaca (Portulaca oleracea cv.) efficiently removes bisphenol A (BPA), an endocrine-disrupting chemical, from a hydroponic solution, but the molecular mechanisms underlying BPA metabolism by portulaca remain unclear. In this study, BPA metabolites converted by portulaca were analyzed by liquid chromatography coupled with tandem mass spectrometry. We observed the hydroxylation of BPA and the oxidization of it to quinone. Polyphenol oxidases are likely to contribute to BPA degradation by portulaca. PMID:22738977

Watanabe, Ippei; Harada, Kazuo; Matsui, Takeshi; Miyasaka, Hitoshi; Okuhata, Hiroshi; Tanaka, Satoshi; Nakayama, Hideki; Kato, Ko; Bamba, Takeshi; Hirata, Kazumasa

2012-01-01

184

Induction of high-frequency somatic embryogenesis in geranium ( Pelargonium x hortorum Bailey cv Ringo Rose) cotyledonary cultures  

Microsoft Academic Search

The cv Ringo Rose of hybrid seed geranium (Pelargonium x hortorum Bailey), previously shown to be recalcitrant in culture, produced somatic embryos when cotyledonary explants were cultured on regeneration medium containing thidiazuron (TDZ), forchlorfenuron (CPPU), or a combination of indole-3-acetic acid and N6 benzylaminopurine (IAA+BAP). Amendment of the basal medium with TDZ (0.5 µM) was the most effective treatment. Addition

B. N. S. Murthy; R. P. Singh; Praveen K. Saxena

1996-01-01

185

Improved antioxidant and anti-inflammatory potential in mice consuming sour cherry juice (Prunus Cerasus cv. Maraska).  

PubMed

The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties. PMID:19763832

Sari?, Ana; Sobocanec, Sandra; Balog, Tihomir; Kusi?, Borka; Sverko, Visnja; Dragovi?-Uzelac, Verica; Levaj, Branka; Cosi?, Zrinka; Macak Safranko, Zeljka; Marotti, Tatjana

2009-12-01

186

Asteroid Lightcurve Analysis at Riverland Dingo Observatory (RDO): 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV 26.  

NASA Astrophysics Data System (ADS)

Lightcurves for five asteroids selected from the Collaborative Asteroid Lightcurve Link (CALL) were obtained at Riverland Dingo Observatory (RDO) from 2012 July-September: 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV26.

Hills, Kevin

2013-01-01

187

Endogenous gibberellin- and cytokinin-like substances in cultured shoot tissues of apple, Malus pumila cv. Jonathan, in relation to adventitious root formation  

Microsoft Academic Search

The frequencies of adventitious root formation in vitro of isolated shoots from bud cultures of apple (Malus pumila cv. Jonathan) after 1, 7 and 31 subcultures (weeks 5, 29 and 109 after the initial culture) were 5, 78 and 95% respectively. Endogenous gibberellin-like substances (GA) were extracted, chromatographed on SiO2 partition columns, and assayed on dwarf rice (Oryza sativa cv.

KIYOTOSHI TAKENOI; John S. Taylor; S. SRISKANDARAJAHZ; P. Pharis Richard; G. Mullins Michael

1982-01-01

188

Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston 1  

PubMed Central

Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived (MD) embryo culture system. TAGs accumulating during embryo development exhibited changes in acyl composition similar to those observed in developing zygotic embryos of the same cv, particularly with respect to erucic and eicosenoic acids. However, MD embryos showed a much higher rate of incorporation of 14C-erucoyl moieties into TAGs in vitro than zygotic embryos. Homogenates of early-late cotyledonary stage MD embryos (14-29 days in culture) were assessed for the ability to incorporate 22:1 and 18:1 (oleoyl) moieties into glycerolipids. In the presence of [1-14C]22:1-coenzyme A (CoA) and various acyl acceptors, including glycerol-3-phosphate (G-3-P), radiolabeled erucoyl moieties were rapidly incorporated into the TAG fraction, but virtually excluded from other Kennedy Pathway intermediates as well as complex polar lipids. This pattern of erucoyl incorporation was unchanged during time course experiments or upon incubation of homogenates with chemicals known to inhibit Kennedy Pathway enzymes. In marked contrast, parallel experiments conducted using [1-14C]18:1-CoA and G-3-P indicated that 14C oleoyl moieties were incorporated into lyso-phosphatidic acids, phosphatidic acids, diacylglycerols, and TAGs of the Kennedy Pathway, as well as other complex polar lipids, such as phosphatidylcholines and phosphatidylethanolamines. When supplied with l-[2-3H(N)]G-3-P and [1-14C]22:1-CoA, the radiolabeled TAG pool contained both isotopes, indicating G-3-P to be a true acceptor of erucoyl moieties. Radio-high-performance liquid chromatography, argentation thin-layer chromatography/gas chromatography-mass spectrometry, and stereospecific analyses of radiolabeled TAGs indicated that 22:1 was selectively incorporated into the sn-3 position by a highly active diacylglycerol acyltransferase (DGAT; EC 2.3.1.20), while oleoyl moieties were inserted into the sn-1 and sn-2 positions. In the presence of sn-1,2-dierucin and [1-14C]22:1-CoA, homogenates and microsomal preparations were able to produce radiolabeled trierucin, a TAG not found endogenously in this species. A 105,000g pellet fraction contained 22:1-CoA:DGAT exhibiting the highest specific activity. The rate of 22:1-CoA:DGAT activity in vitro could more than account for the maximal rate of TAG biosynthesis observed in vivo during embryo development. In double label experiments, G-3-P was shown to stimulate the conversion of [3H]phosphatidylcholines to [3H]diacylglycerols, which subsequently acted as acceptors for 14C erucoyl moieties. In vitro, 22:1 moieties did not enter the sn-1 position of TAGs by a postsynthetic modification or transacylation of preformed TAGs. PMID:16668417

Taylor, David C.; Weber, Nikolaus; Barton, Dennis L.; Underhill, Edward W.; Hogge, Lawrence R.; Weselake, Randall J.; Pomeroy, M. Keith

1991-01-01

189

Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation  

PubMed Central

Background Ultraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV-B treatment. Some reports have analyzed the changes in gene expression caused by UV-B light on several plant species using microarray technology. However, there is no information on the transcriptome response triggered by UV-B in grapevine. In this paper we investigate the gene expression responses of leaves from in vitro cultured Vitis vinifera cv. Malbec plants subjected to the same dose of biologically effective UV-B radiation (4.75 kJ m-2 d-1) administered at two different fluence rates (16 h at ? 8.25 ?W cm-2, 4 h at ? 33 ?W cm-2) using a new custom made GrapeGen Affymetrix GeneChip®. Results The number of genes modulated by high fluence rate UV-B doubled the number of genes modulated by low fluence UV-B. Their functional analyses revealed several functional categories commonly regulated by both UV-B treatments as well as categories more specifically modulated depending on UV-B fluence rate. General protective responses, namely the induction of pathways regulating synthesis of UV-B absorbing compounds such as the Phenylpropanoid pathway, the induction of different antioxidant defense systems and the activation of pathways commonly associated with pathogen defense and abiotic stress responses seem to play critical roles in grapevine responses against UV-B radiation. Furthermore, high fluence rate UV-B seemed to specifically modulate additional pathways and processes in order to protect grapevine plantlets against UV-B-induced oxidative stress, stop the cell cycle progression, and control protein degradation. On the other hand, low fluence rate UV-B regulated the expression of specific responses in the metabolism of auxin and abscisic acid as well as in the modification of cell walls that could be involved in UV-B acclimation-like processes. Conclusion Our results show the UV-B radiation effects on the leaf transcriptome of grapevine (Vitis vinifera cv. Malbec) plantlets. Functional categories commonly modulated under both UV-B treatments as well as transcripts specifically regulated in an UV-B-intensity dependent way were identified. While high fluence rate UV-B had regulatory effects mainly on defense or general multiple-stress responses pathways, low fluence rate UV-B promoted the expression of genes that could be involved in UV-B protection or the amelioration of the UV-B-induced damage. This study also provides an extensive list of genes regulating multiple metabolic pathways involved in the response of grapevine to UV-B that can be used for future researches. PMID:20959019

2010-01-01

190

Establishment of regeneration and transformation system in Egyptian sesame (Sesamum indicum L.) cv Sohag 1.  

PubMed

Sesame (Sesamum indicum L.) is an important oil crop in many tropical and sub-tropical regions of the world, yet has received little attention in applying modern biotechnology in its improvement due to regeneration and transformation difficulties. Here within, we report the successful production of transgenic fertile plants of sesame (cv Sohag 1), after screening several cultivars. Agrobacterium tumefaciens- carrying the pBI121 plasmid {neomycin phosphotransferase gene (NPTII) and a ?-glucuronidase gene (GUS)} was used in all experiments. Recovery of transgenic sesame shoots was achieved using shoot induction medium (Murashige and Skoog MS basal salt mixture + Gamborg's B5 vitamins + 2.0 mg/l BA + 1.0 mg/l IAA + 5.0 mg/l AgNO3 + 30.0 g/l sucrose + 7.0 g/l agar + 200 mg/l cefotaxime and 25 mg/l kanamycin) and shoots were rooted on MS medium + B5 vitamins + 1.0 mg/l IAA + 10.0 g/l sucrose and 7.0 g/l agar. Rooted shoots were transplanted into soil and grown to maturity in greenhouse. Incorporation and expression of the GUS gene into T0 sesame plants was confirmed using polymerase chain reaction (PCR), reverse transcriptase-PCR (RT-PCR) and GUS histochemical assay. Several factors were found to be important for regeneration and transformation in sesame. The most effective were plant genotype and the addition of AgNO3 for successful recovery of sesame shoots. Co-cultivation time and optical density of the Agrobacterium were also critical for sesame transformation. This work is an attempt to open the door for further genetic improvement of sesame using important agronomic traits. PMID:22179191

Al-Shafeay, Amal F; Ibrahim, Ahmed S; Nesiem, Mohamed R; Tawfik, Mohamed S

2011-01-01

191

Nitrogen supply affects anthocyanin biosynthetic and regulatory genes in grapevine cv. Cabernet-Sauvignon berries.  

PubMed

Accumulation of anthocyanins in grape berries is influenced by environmental factors (such as temperature and light) and supply of nutrients, i.e., fluxes of carbon and nitrogen feeding the berry cells. It is established that low nitrogen supply stimulates anthocyanin production in berry skin cells of red varieties. The present works aims to gain a better understanding of the molecular mechanisms involved in the response of anthocyanin accumulation to nitrogen supply in berries from field grown-plants. To this end, we developed an integrated approach combining monitoring of plant nitrogen status, metabolite measurements and transcript analysis. Grapevines (cv. Cabernet-Sauvignon) were cultivated in a vineyard with three nitrogen fertilization levels (0, 60 and 120 kg ha(-1) of nitrogen applied on the soil). Anthocyanin profiles were analyzed and compared with gene expression levels. Low nitrogen supply caused a significant increase in anthocyanin levels at two ripening stages (26 days post-véraison and maturity). Delphinidin and petunidin derivatives were the most affected compounds. Transcript levels of both structural and regulatory genes involved in anthocyanin synthesis confirmed the stimulation of the phenylpropanoid pathway. Genes encoding phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavonoid-3',5'-hydroxylase (F3'5'H), dihydroflavonol-4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) exhibited higher transcript levels in berries from plant cultivated without nitrogen compared to the ones cultivated with 120 kg ha(-1) nitrogen fertilization. The results indicate that nitrogen controls a coordinated regulation of both positive (MYB transcription factors) and negative (LBD proteins) regulators of the flavonoid pathway in grapevine. PMID:24735825

Soubeyrand, Eric; Basteau, Cyril; Hilbert, Ghislaine; van Leeuwen, Cornelis; Delrot, Serge; Gomès, Eric

2014-07-01

192

Nature and origin of interstellar diamond from the Allende CV3 meteorite  

NASA Technical Reports Server (NTRS)

Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende CV3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on 3 mm EM grids. The AEM was performed on transmission-thin fragments of the material which overlay holes in the film, to eliminate interference from the substrate. Electron Spectroscopy for Chemical Analysis (ESCA) was performed on a large aliquot of C. Diamond was identified by selected area electron diffraction. Scanning Transmission Electron Microscope / Energy Dispersive X-ray (STEM-EDS) microanalyses of the C delta diamond, using a light-element detector, show that oxygen and possibly nitrogen are the only impurities consistently present. ESCA spectra from bulk C delta material confirm the presence of N at a level of 0.35 percent or less. Under UV irradiation a yellow-red fluorescence is observed, consistent with that of natural diamonds containing substitutional N. Electron Energy Loss Spectra (EELS) were recorded at 2 eV resolution from the C delta diamond, high pressure synthetic diamond, a diamond film produced in a low pressure plasma by chemical vapor deposition (CVD) on a heated silicon substrate (Roy, 1987), graphite, and amorphous arc sputtered carbon. Comparison of the carbon K edge shape and fine structure shows the Allende C delta phase to be largely diamond, but with a significant pre-edge absorption feature indicative of transitions of C 1s electrons into pi asterisk orbitals which are absent in the purely sp(3)-bonded diamond but present in graphite and amorphous carbon.

Blake, David; Freund, Friedemann; Bunch, Ted; Krishnan, Kannan; Stampfer, Mitch; Chang, Sherwood; Tielens, Alexander G. G. M.

1990-01-01

193

Nature and origin of interstellar diamond from the Allende CV3 meteorite  

NASA Astrophysics Data System (ADS)

Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende CV3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on 3 mm EM grids. The AEM was performed on transmission-thin fragments of the material which overlay holes in the film, to eliminate interference from the substrate. Electron Spectroscopy for Chemical Analysis (ESCA) was performed on a large aliquot of C. Diamond was identified by selected area electron diffraction. Scanning Transmission Electron Microscope / Energy Dispersive X-ray (STEM-EDS) microanalyses of the C delta diamond, using a light-element detector, show that oxygen and possibly nitrogen are the only impurities consistently present. ESCA spectra from bulk C delta material confirm the presence of N at a level of 0.35 percent or less. Under UV irradiation a yellow-red fluorescence is observed, consistent with that of natural diamonds containing substitutional N. Electron Energy Loss Spectra (EELS) were recorded at 2 eV resolution from the C delta diamond, high pressure synthetic diamond, a diamond film produced in a low pressure plasma by chemical vapor deposition (CVD) on a heated silicon substrate (Roy, 1987), graphite, and amorphous arc sputtered carbon. Comparison of the carbon K edge shape and fine structure shows the Allende C delta phase to be largely diamond, but with a significant pre-edge absorption feature indicative of transitions of C 1s electrons into pi asterisk orbitals which are absent in the purely sp(3)-bonded diamond but present in graphite and amorphous carbon.

Blake, David; Freund, Friedemann; Bunch, Ted; Krishnan, Kannan; Stampfer, Mitch; Chang, Sherwood; Tielens, Alexander G. G. M.

1990-04-01

194

Solanum lycopersicum cv. Heinz 1706 chromosome 6: distribution and abundance of genes and retrotransposable elements.  

PubMed

We studied the physical and genetic organization of chromosome 6 of tomato (Solanum lycopersicum) cv. Heinz 1706 by combining bacterial artificial chromosome (BAC) sequence analysis, high-information-content fingerprinting, genetic analysis, and BAC-fluorescent in situ hybridization (FISH) mapping data. The chromosome positions of 81 anchored seed and extension BACs corresponded in most cases with the linear marker order on the high-density EXPEN 2000 linkage map. We assembled 25 BAC contigs and eight singleton BACs spanning 2.0 Mb of the short-arm euchromatin, 1.8 Mb of the pericentromeric heterochromatin and 6.9 Mb of the long-arm euchromatin. Sequence data were combined with their corresponding genetic and pachytene chromosome positions into an integrated map that covers approximately a third of the chromosome 6 euchromatin and a small part of the pericentromeric heterochromatin. We then compared physical length (Mb), genetic (cM) and chromosome distances (microm) for determining gap sizes between contigs, revealing relative hot and cold spots of recombination. Through sequence annotation we identified several clusters of functionally related genes and an uneven distribution of both gene and repeat sequences between heterochromatin and euchromatin domains. Although a greater number of the non-transposon genes were located in the euchromatin, the highly repetitive (22.4%) pericentromeric heterochromatin displayed an unexpectedly high gene content of one gene per 36.7 kb. Surprisingly, the short-arm euchromatin was relatively rich in repeats as well, with a repeat content of 13.4%, yet the ratio of Ty3/Gypsy and Ty1/Copia retrotransposable elements across the chromosome clearly distinguished euchromatin (2:3) from heterochromatin (3:2). PMID:19207213

Peters, Sander A; Datema, Erwin; Szinay, Dóra; van Staveren, Marjo J; Schijlen, Elio G W M; van Haarst, Jan C; Hesselink, Thamara; Abma-Henkens, Marleen H C; Bai, Yuling; de Jong, Hans; Stiekema, Willem J; Klein Lankhorst, René M; van Ham, Roeland C H J

2009-06-01

195

Mercury abundances and isotopic compositions in the Murchison (CM) and Allende (CV) carbonaceous chondrites  

NASA Astrophysics Data System (ADS)

The abundance and isotopic composition of Hg was determined in bulk samples of both the Murchison (CM) and Allende (CV) carbonaceous chondrites using single- and multi-collector inductively coupled plasma mass spectrometry (ICP-MS). The bulk abundances of Hg are 294 ± 15 ng/g in Murchison and 30.0 ± 1.5 ng/g in Allende. These values are within the range of previous measurements of bulk Hg abundances by neutron activation analysis (NAA). Prior studies suggested that both meteorites contain isotopically anomalous Hg, with ? 196/202Hg values for the anomalous, thermal-release components from bulk samples ranging from -260 ‰ to +440 ‰ in Murchison and from -620 ‰ to +540 ‰ in Allende (Jovanovic and Reed, 1976a; 1976b; Kumar and Goel, 1992). Our multi-collector ICP-MS measurements suggest that the relative abundances of all seven stable Hg isotopes in both meteorites are identical to terrestrial values within 0.2 to 0.5 ‰. On-line thermal-release experiments were performed by coupling a programmable oven with the single-collector ICP-MS. Powdered aliquots of each meteorite were linearly heated from room temperature to 900°C over twenty-five minutes under an Ar atmosphere to measure the isotopic composition of Hg released from the meteorites as a function of temperature. In separate experiments, the release profiles of S and Se were determined simultaneously with Hg to constrain the Hg distribution within the meteorites and to evaluate the possibility of Se interferences in previous NAA studies. The Hg-release patterns differ between Allende and Murchison. The Hg-release profile for Allende contains two distinct peaks, at 225° and 343°C, whereas the profile for Murchison has only one peak, at 344°C. No isotopically anomalous Hg was detected in the thermal-release experiments at a precision level of 5 to 30 ‰, depending on the isotope ratio. In both meteorites the Hg peak at ˜340°C correlates with a peak in the S-release profile. This correlation suggests that Hg is associated with S-bearing phases and, thus, that HgS is a major Hg-bearing phase in both meteorites. The Hg peak at 225°C for Allende is similar to release patterns of physically adsorbed Hg on silicate and metal grains. Prior studies suggested that the isotopic anomalies reported from NAA resulted from interference between 203Hg and 75Se. However, the amount of Se released from both meteorites, relative to Hg, is insufficient to produce all of the observed anomalies.

Lauretta, Dante S.; Klaue, Bjoern; Blum, Joel D.; Buseck, Peter R.

2001-09-01

196

In vitro selection and field responses of somaclonal variant plants of rice cv PR113 for drought tolerance  

PubMed Central

Drought is the major environmental stress that limits rice productivity worldwide. In vitro somaclonal variation using different selection agents has been used for crop improvement. Here, rice plants of cv PR113 were selected in vitro on 30, 50 and 70 g L-1 polyethylene glycol 6,000 (PEG). Callus growth, proliferation, calli volume (first and second culture) and plantlet regeneration (third culture) were found to be decreased upto a certain level to acquire tolerance to PEG-induced drought. From the field data, 30 g L-1 PEG lines showed higher vegetative growth (plant height, tiller number, leaf number, shoot weight and root growth) as compared with 50 g L-1 PEG selected somaclone lines under limited irrigation. The yield parameters-panicle length, panicle weight, grains per panicle, 1,000-grain weight, grain yield per plant, harvest index and grain straw ratio were also higher in 30 g L-1 PEG lines as compared with 50 g L-1 PEG lines. The results, therefore indicate that 30 g L-1 PEG selected somaclone lines were more suited than 50 g L-1 PEG selected somaclone lines under stress as compared with WT. The finding suggests that rice cv PR113 somaclones generated on PEG are found to be drought tolerant under field condition with better yield. PMID:23333982

Verma, Dipti; Ansari, Mohammad Wahid; Agrawal, Ganesh Kumar; Rakwal, Randeep; Shukla, Alok; Tuteja, Narendra

2013-01-01

197

Processor core for real time background identification of HD video based on OpenCV Gaussian mixture model algorithm  

NASA Astrophysics Data System (ADS)

The identification of moving objects is a fundamental step in computer vision processing chains. The development of low cost and lightweight smart cameras steadily increases the request of efficient and high performance circuits able to process high definition video in real time. The paper proposes two processor cores aimed to perform the real time background identification on High Definition (HD, 1920 1080 pixel) video streams. The implemented algorithm is the OpenCV version of the Gaussian Mixture Model (GMM), an high performance probabilistic algorithm for the segmentation of the background that is however computationally intensive and impossible to implement on general purpose CPU with the constraint of real time processing. In the proposed paper, the equations of the OpenCV GMM algorithm are optimized in such a way that a lightweight and low power implementation of the algorithm is obtained. The reported performances are also the result of the use of state of the art truncated binary multipliers and ROM compression techniques for the implementation of the non-linear functions. The first circuit has commercial FPGA devices as a target and provides speed and logic resource occupation that overcome previously proposed implementations. The second circuit is oriented to an ASIC (UMC-90nm) standard cell implementation. Both implementations are able to process more than 60 frames per second in 1080p format, a frame rate compatible with HD television.

Genovese, Mariangela; Napoli, Ettore

2013-05-01

198

Potential for phytoextraction of copper by Sinapis alba and Festuca rubra cv. Merlin grown hydroponically and in vineyard soils.  

PubMed

The extensive use of copper-bearing fungicides in vineyards is responsible for the accumulation of copper (Cu) in soils. Grass species able to accumulate Cu could be cultivated in the vineyard inter-rows for copper phytoextraction. In this study, the capacity of Festuca rubra cv Merlin and Sinapis alba to tolerate and accumulate copper (Cu) was first investigated in a hydroponic system without the interference of soil chemical-physical properties. After the amendment of Cu (5 or 10 mg Cu l-(1)) to nutrient solution, shoot Cu concentration in F. rubra increased up to 108.63 mg Cu kg(-1) DW, more than three times higher than in S. alba (31.56 mg Cu kg(-1) DW). The relationship between Cu concentration in plants and external Cu was dose-dependent and species specific. Results obtained from the hydroponic experiment were confirmed by growing plants in pots containing soil collected from six Italian vineyards. The content of soil organic matter was crucial to enhance Cu tolerance and accumulation in the shoot tissues of both plant species. Although S. alba produced more biomass than F. rubra in most soils, F. rubra accumulated significantly more Cu (up to threefold to fourfold) in the shoots. Given these results, we recommended that F. rubra cv Merlin could be cultivated in the vineyard rows to reduce excess Cu in vineyard soils. PMID:24234763

Malagoli, Mario; Rossignolo, Virginia; Salvalaggio, Nico; Schiavon, Michela

2014-03-01

199

Phosphatidylinositol 4,5-Bisphosphate Induces Actin Stress-Fiber Formation and Inhibits Membrane Ruffling in Cv1 Cells  

PubMed Central

Phosphatidylinositol 4,5 bisphosphate (PIP2) is widely implicated in cytoskeleton regulation, but the mechanisms by which PIP2 effect cytoskeletal changes are not defined. We used recombinant adenovirus to infect CV1 cells with the mouse type I phosphatidylinositol phosphate 5-kinase ? (PIP5KI), and identified the players that modulate the cytoskeleton in response to PIP2 signaling. PIP5KI overexpression increased PIP2 and reduced phosphatidylinositol 4 phosphate (PI4P) levels. It promoted robust stress-fiber formation in CV1 cells and blocked PDGF-induced membrane ruffling and nucleated actin assembly. Y-27632, a Rho-dependent serine/threonine protein kinase (ROCK) inhibitor, blocked stress-fiber formation and inhibited PIP2 and PI4P synthesis in cells. However, Y-27632 had no effect on PIP2 synthesis in lysates, although it inhibited PI4P synthesis. Thus, ROCK may regulate PIP2 synthesis by controlling PI4P availability. PIP5KI overexpression decreased gelsolin, profilin, and capping protein binding to actin and increased that of ezrin. These changes can potentially account for the increased stress fiber and nonruffling phenotype. Our results establish the physiological role of PIP2 in cytoskeletal regulation, clarify the relation between Rho, ROCK, and PIP2 in the activation of stress-fiber formation, and identify the key players that modulate the actin cytoskeleton in response to PIP2. PMID:11238445

Yamamoto, Masaya; Hilgemann, Donald H.; Feng, Siyi; Bito, Haruhiko; Ishihara, Hisamitsu; Shibasaki, Yoshikazu; Yin, Helen L.

2001-01-01

200

[Transformation of embryogenic Calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) mediated by agrobacterium].  

PubMed

Formation of embryogenic calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) was induced from mature seeds as explants, and proliferated on MS medium containing 2,4-D 5.0 mg/L and KT 0.05 mg/L. An effective and stable callus regeneration system was established by optimizing the culture conditions (Tables 1, 2 and Fig.2). After the calli were subcultured 8 weeks, selected the whitish-yellow-coloured compact nodular calli that transformed with plasmid pCAMBIA1304 carrying hygromycin resistance gene (hptII) and Pseudomonas pseudoalcaligenes insecticidal protein gene (ppIP), which was mediated by an Agrobacterium strain EHA105. Resistant plants were obtained after hygromycin selection (Figs.3, 4). Some important factors that affect the transformation efficiency were studied, which included selection pressure, time of embryogenic calli proliferation, OD value of Agrobacterium suspension, temperature, medium and time of co-cultivation, and concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformation plant regeneration. This research is the first successful genetic transformation of Elymus sibiricus L. cv. Chuancao No.2 mediated by Agrobacterium. PMID:16477130

Li, Da-Xu; Zhang, Jie; Zhao, Jian; Zhang, Yi; Li, Li; Liu, Su-Jun; Chen, Fei; Yang, Zhi-Rong

2006-02-01

201

Interpretation of F106B and CV580 in-flight lightning data and form factor determination  

NASA Technical Reports Server (NTRS)

Two topics of in-flight aircraft/lightning interaction are addressed. The first is the analysis of measured data from the NASA F106B Thunderstorm Research Aircraft and the CV580 research program run by the FAA and Wright-Patterson Air Force Base. The CV580 data was investigated in a mostly qualitative sense, while the F106B data was subjected to both statistical and quantitative analysis using linear triggered lightning finite difference models. The second main topic is the analysis of field mill data and the calibration of the field mill systems. The calibration of the F106B field mill system was investigated using an improved finite difference model of the aircraft having a spatial resolution of one-quarter meter. The calibration was applied to measured field mill data acquired during the 1985 thunderstorm season. The experimental determination of form factors useful for field mill calibration was also investigated both experimentally and analytically. The experimental effort involved the use of conducting scale models and an electrolytic tank. An analytic technique was developed to aid in the understanding of the experimental results.

Rudolph, T.; Horembala, J.; Eriksen, F. J.; Weigel, H. S.; Elliott, J. R.; Parker, S. L.; Perala, R. A.

1989-01-01

202

CV Shift Characteristics of Metal-Al2O3Si and Metal-Al2O3SiO2Si Structures  

Microsoft Academic Search

C-V shift characteristics of MAS(Metal\\/Al2O3\\/Si) structure with Al2O3 prepared by the hydrolysis of AlCl3 are investigated as a function of the Al2O3 thickness, the temperature and the applied pulse width. Using also MAOS(Metal\\/Al2O3\\/SiO2\\/Si) structure the critical voltage required to shift the C-V curve is given as a function of the SiO2 thickness. The results show that the critical voltage of

Tohru Tsujide

1972-01-01

203

Transactivation of progestin- and estrogen-responsive promoters by 19-nor progestins in African Green Monkey Kidney CV1 cells.  

PubMed

New and more potent progestins and antiprogestins suitable for reproductive therapy and contraception are currently the target of intensive research. The design of such drugs has been hampered by the complex technology required for screening these compounds at the molecular level. To solve this problem, we developed an in vitro cell system that allows detection of the progestagenic effects of a given compound using a PRE2-TATA-CAT reporter vector transiently introduced in a cell line stably transfected with the rabbit progesterone receptor (PR). The African Green Monkey Kidney CV1 (AGMK-CV1) cell line was chosen because these cells do not express endogenous steroid receptors; the selected clone stably expressing the rabbit PR has been maintained in our laboratory for more than 2 yr without detectable losses in PR content and progestagenic response. The presence and function of the PR were assessed by immunohistochemical and saturation analyses as well as by monitoring transactivation of the PRE2-TATA-CAT reporter gene. In this cell line, the PR is expressed at a concentration of 0.170 fmol/mg of protein, and the receptor is localized within the cell nucleus in either the presence or absence of the potent synthetic progestin R5020. This PR-expressing cell system allowed study of the in vitro progestational activity of several 19-nor progestins. The antiprogestin RU486 inhibited CAT activity induced by R5020; norethisterone (NET), levonorgestrel (LNG), and gestodene (GSD) induced PRE2-TATA-CAT activity at concentrations similar to those of R5020, whereas NET A-ring-reduced metabolites induced CAT activity at an extent lower than (5alpha-NET) or similar (3beta,5alpha-NET) to that of the precursor compound. The PRE2-TATA-CAT induction by 17beta-estradiol was also analyzed and no crossreactivity was detected. However, when the ERE-VitA2-TK-CAT (estrogen-responsive element-vitellogenin A2-thymidine kinase promoter-CAT) reporter vector and the estradiol receptor alpha or beta were cotransfected, CAT activity was induced in the presence of 17beta-estradiol, and NET tetrahydro-reduced derivatives. The results indicate that this AGMK-CV1-PR cell assay system appears to be suitable for measuring the effects of different synthetic progestins at the transcriptional level. In this assay system, NET, LNG, and GSD exhibit potent progestational effects at the transcriptional level. In the particular case of NET, the assay system allowed us to determine that the single or multiple hormonal transcriptional effects of this compound are partially mediated by its A-ring-reduced derivatives. PMID:11954666

Pasapera, A M; Gutiérrez-Sagal, R; García-Becerra, R; Ulloa-Aguirre, A; Savouret, J F

2001-12-01

204

The Anatomy and Bulk Composition of CAI Rims in the Vigarano (CV3) Chondrite  

NASA Astrophysics Data System (ADS)

A striking feature of Ca,Al-rich inclusions (CAIs) in chondrites is the presence of mineralogical layers that typically form rim sequences up to 50 micrometers thick [1]. Many ideas regarding the origin of CAI rims have been proposed, but none are entirely satisfactory. The detailed mineralogy and bulk compositions of relatively unaltered CAI rims in the Vigarano (CV3) chondrite described here provide constraints on hypotheses of rim formation. Rim Mineralogy: CAIs in Vigarano consist of melilite (mel)- and spinel (sp)- rich varieties, both of which are rimmed [2]. Around mel-rich objects, the layer sequence is CAI interior --> sp-rich layer (sometimes absent) --> mel/anorthite (anor) layer --> Ti-Al-rich clinopyroxene (Tpx) layer --> Al- diopside (Al-diop) layer --> olivine (ol) +/- Al-diop layer --> host matrix. The sequence around sp-rich objects differs from this in that the mel/anor layer is absent. Both the sp-rich layer around mel-cored CAIs and the cores of sp-rich CAIs in Vigarano are largely comprised of a fine-grained (<=1 micrometer) intergrowth of sp, Tpx, and minor mel and perovskite. These intergrowths are typically so fine grained that little internal texture is discernible. Mixing calculations suggest the presence of ~10 vol% Tpx in the sp-rich layer of two mel-cored CAIs, and the presence of ~35 vol% Tpx within one sp-cored CAI. The mel/anor layer is sometimes monomineralic, consisting of mel alone, or bimineralic, consisting of both mel and anor. Where bimineralic, anor typically occurs in the outer part of the layer. In places, anor (An(sub)99-100) has partially altered to nepheline and voids. Rim mel is systematically less gehlenitic than mel in the CAI interiors, especially compared to mel in the interior adjacent to the rims. The Tpx layer (>2 and up to 15 wt% TiO2) and Al-diop layer (<2 wt% TiO2) are monomineralic and show chemical zoning trends radial to the CAIs. Moving outward, TiO2 and Al2O3 generally decrease, while SiO2 and MgO increase, although Al2O3 shows a small concentration maximum in the Al-diop layer. High-quality EMPA data suggest that Ti^3+/Ti^4+ decreases outward in the Tpx layer, and that Fe^3+ is present in the Al-diop layer, implying that a steep gradient in oxidation state occurs across these two layers. The ol layer is comprised of individually zoned grains (<=5 micrometers across) that have forsteritic cores and thin (<=1 micrometer) rims of more ferrous ol. The ol grains often form triple-grain junctions and occasionally form clusters that are enclosed by Al-diop. The texture of this polycrystalline layer suggests that it formed by the attachment of preexisting ol grains onto the surfaces of CAIs, and the triple-junctions and steep zoning profiles of the ol grains suggest that they were annealed in a short-lived heating event. Bulk Rim Composition: Bulk rim compositions for several mel-rich CAIs were determined by using EMPA traverses across representative portions of the rims. These compositions plot within the sp + forsterite (fo) field of the gehlenite-anorthite-forsterite ternary diagram of Stolper [3], and are unattainable by the igneous crystallization of a mel-rich CAI composition. Moreover, a hypothetical melt with the composition of the rims has a predicted crystallization sequence (sp --> sp + fo --> sp + fo + anor or mel or Tpx) that does not correspond to observed rim sequences. It thus appears that (1) the rim region did not form through crystallization of molten CAIs; and (2) rim layers did not originate solely by the crystallization of a melt layer present on a solid CAI core [4,5]. References: [1] Wark D. A. and Lovering J. F. (1977) Proc. LSC 8th, 95-112. [2] Ruzicka A. and Boynton W. V. (1991) Meteoritics, 26, 390-391. [3] Stolper E. (1982) GCA, 46, 2159-2180. [4] Korina M. I. et al. (1982) LPS XIII, 399- 400. [5] Bunch T. E. and Chang S. (1980) Meteoritics, 15, 270- 271.

Ruzicka, A.; Boynton, W. V.

1993-07-01

205

Complete genome sequence of a banana bract mosaic virus isolate infecting the French plantain cv. Nendran in India.  

PubMed

The first complete genome sequence of an Indian isolate (TRY) of Banana bract mosaic virus (BBrMV) was determined following virus RNA extraction from the French plantain cv. Nendran (AAB). The complete genome was 9711 nucleotides excluding the poly(A) tail and had a genome organization similar to that of a Philippine (PHI) isolate characterized earlier. When compared to BBrMV-PHI, the complete genome sequence of BBrMV-TRY was 94% identical at the nucleotide level and its ten mature proteins had amino acid sequence identities ranging from 88 to 98%. Phylogenetic analysis suggests that the BBrMV-TRY isolate is closely related to the BBrMV-PHI isolate. PMID:22134527

Balasubramanian, V; Selvarajan, R

2012-02-01

206

Direct identification and characterization of phenolic compounds from crude extracts of buds and internodes of grapevine (Vitis vinifera cv Merlot).  

PubMed

The crude methanol extracts of latent buds and internodes Vitis vinifera L. cv. Merlot were used for the determination of phenolic compounds by a combination of reverse phase HPLC with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). This method allowed the identification of 9 phenolic compounds without purification or fractionation. These 9 compounds were divided into three groups: procyanidins, flavonols and stilbenes. Detection by HPLC-DAD at different wave lengths of 280 nm to 320 nm, allowed the estimation of concentrations of those compounds. This method permitted, for the first time, both characterization and quantification of polyphenolic compounds in buds of grapevine. Comparison with the results obtained in internodes showed that quercetin, resveratrol tetramer and ?-viniferin had similar levels in buds and internodes while six other compounds identified had higher levels in buds. PMID:25532283

Qsaib, Said; Mateus, Nuno; Ikbal, Fatima Ez-zohra; Rifai, Lala Aicha; De Freitas, Victor; Koussa, Tayeb

2014-11-01

207

Induction of high-frequency somatic embryogenesis in geranium (Pelargonium x hortorum Bailey cv Ringo Rose) cotyledonary cultures.  

PubMed

The cv Ringo Rose of hybrid seed geranium (Pelargonium x hortorum Bailey), previously shown to be recalcitrant in culture, produced somatic embryos when cotyledonary explants were cultured on regeneration medium containing thidiazuron (TDZ), forchlorfenuron (CPPU), or a combination of indole-3-acetic acid and N(6) benzylaminopurine (IAA+BAP). Amendment of the basal medium with TDZ (0.5 ?M) was the most effective treatment. Addition of amino acids to the medium promoted the growth of somatic embryos. Retention of the proximal region of the cotyledon was crucial for regeneration, but the removal of the distal 1/3 to 1/2 cotyledon had no significant effect on somatic embryogenesis. Cotyledonary explants formed somatic embryos in higher frequency and much earlier than hypocotyl explants cultured on the same medium. The somatic embryos induced on cotyledonary explants were germinated on basal medium. More than 70% of the somatic embryos were converted into plants and transferred to soil. PMID:24178422

Murthy, B N; Singh, R P; Saxena, P K

1996-02-01

208

Whole-rock 26Al-26Mg systematics of amoeboid olivine aggregates from the oxidized CV3 carbonaceous chondrite Allende  

NASA Astrophysics Data System (ADS)

We report on mineralogy, petrography, and whole-rock 26Al-26Mg systematics of eight amoeboid olivine aggregates (AOAs) from the oxidized CV chondrite Allende. The AOAs consist of forsteritic olivine, opaque nodules, and variable amounts of Ca,Al-rich inclusions (CAIs) of different types, and show evidence for alteration to varying degrees. Melilite and anorthite are replaced by nepheline, sodalite, and grossular; spinel is enriched in FeO; opaque nodules are replaced by Fe,Ni-sulfides, ferroan olivine and Ca,Fe-rich pyroxenes; forsteritic olivine is enriched in FeO and often overgrown by ferroan olivine. The AOAs are surrounded by fine-grained, matrix-like rims composed mainly of ferroan olivine and by a discontinuous layer of Ca,Fe-rich silicates. These observations indicate that AOAs experienced in situ elemental open-system iron-alkali-halogen metasomatic alteration during which Fe, Na, Cl, and Si were introduced, whereas Ca was removed from AOAs and used to form the Ca,Fe-rich silicate rims around AOAs. The whole-rock 26Al-26Mg systematics of the Allende AOAs plot above the isochron of the whole-rock Allende CAIs with a slope of (5.23 ± 0.13) × 10-5 reported by Jacobsen et al. (2008). In contrast, whole-rock 26Al-26Mg isotope systematics of CAIs and AOAs from the reduced CV chondrite Efremovka define a single isochron with a slope of (5.25± 0.01) × 10-5 (Larsen et al. 2011). We infer that the excesses in 26Mg* present in Allende AOAs are due to their late-stage open-system metasomatic alteration. Thus, the 26Al-26Mg isotope systematics of Allende CAIs and AOAs are disturbed by parent body alteration processes, and may not be suitable for high-precision chronology of the early solar system events and processes.

Olsen, M. B.; Krot, A. N.; Larsen, K.; Paton, C.; Wielandt, D.; Schiller, M.; Bizzarro, M.

2011-11-01

209

Identification and characterization of an adeno-associated virus integration site in CV-1 cells from the African green monkey.  

PubMed

Adeno-associated virus (AAV) is a classification given to a group of nonpathogenic, single-stranded DNA viruses known to reside latently in primates. During latency in humans, AAV type 2 (AAV2) preferentially integrates at a site on chromosome 19q13.3ter by targeting a sequence composed of an AAV Rep binding element (RBE), a spacer, and a nicking site. Here, we report the DNA sequence of an African green monkey AAV integration site isolated from CV-1 cells. Overall, it has 98% homology to the analogous human site, including identical spacer and nicking sequences. However, the simian RBE is expanded, having five perfect directly repeated GAGC tetramers. We carried out a number of in vitro and in vivo assays to determine the effect of this expanded RBE sequence on the Rep-RBE interaction and AAV targeted integration. Using electromobility shift assays it was demonstrated that AAV4 Rep68 bound the expanded RBE with a sixfold-greater affinity than the human RBE. To determine the basis for the affinity increase, DNase I protection and methylation interference (MI) assays were performed. Comparison of footprints on both the human and simian RBEs revealed nearly identical protection; however, MI analysis suggested greater interaction with the guanine nucleotides of the expanded RBE, thus providing a biochemical basis for the increased binding activity. In vivo, integration targeted to the simian RBE was demonstrated by PCR analysis of latently infected Cos-7 cells. Interestingly, the frequency of site-specific integration was twofold greater in Cos-7 cells than in HeLa cells. Overall, these experiments establish that the simian RBE, identified in CV-1 cells, functions analogously to the human RBE and provide further evidence for a developing model that proposes individual roles for the RBE and the spacer and nicking site elements. PMID:12525625

Amiss, Terry J; McCarty, Doug M; Skulimowski, Anna; Samulski, R Jude

2003-02-01

210

Identification and Characterization of an Adeno-Associated Virus Integration Site in CV-1 Cells from the African Green Monkey  

PubMed Central

Adeno-associated virus (AAV) is a classification given to a group of nonpathogenic, single-stranded DNA viruses known to reside latently in primates. During latency in humans, AAV type 2 (AAV2) preferentially integrates at a site on chromosome 19q13.3ter by targeting a sequence composed of an AAV Rep binding element (RBE), a spacer, and a nicking site. Here, we report the DNA sequence of an African green monkey AAV integration site isolated from CV-1 cells. Overall, it has 98% homology to the analogous human site, including identical spacer and nicking sequences. However, the simian RBE is expanded, having five perfect directly repeated GAGC tetramers. We carried out a number of in vitro and in vivo assays to determine the effect of this expanded RBE sequence on the Rep-RBE interaction and AAV targeted integration. Using electromobility shift assays it was demonstrated that AAV4 Rep68 bound the expanded RBE with a sixfold-greater affinity than the human RBE. To determine the basis for the affinity increase, DNase I protection and methylation interference (MI) assays were performed. Comparison of footprints on both the human and simian RBEs revealed nearly identical protection; however, MI analysis suggested greater interaction with the guanine nucleotides of the expanded RBE, thus providing a biochemical basis for the increased binding activity. In vivo, integration targeted to the simian RBE was demonstrated by PCR analysis of latently infected Cos-7 cells. Interestingly, the frequency of site-specific integration was twofold greater in Cos-7 cells than in HeLa cells. Overall, these experiments establish that the simian RBE, identified in CV-1 cells, functions analogously to the human RBE and provide further evidence for a developing model that proposes individual roles for the RBE and the spacer and nicking site elements. PMID:12525625

Amiss, Terry J.; McCarty, Doug M.; Skulimowski, Anna; Samulski, R. Jude

2003-01-01

211

In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.  

PubMed

An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) ?-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana. PMID:24222501

Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert

2014-02-01

212

The genetics of domestication of yardlong bean, Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis  

PubMed Central

Background and Aims The genetics of domestication of yardlong bean [Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis] is of particular interest because the genome of this legume has experienced divergent domestication. Initially, cowpea was domesticated from wild cowpea in Africa; in Asia a vegetable form of cowpea, yardlong bean, subsequently evolved from cowpea. Information on the genetics of domestication-related traits would be useful for yardlong bean and cowpea breeding programmes, as well as comparative genome study among members of the genus Vigna. The objectives of this study were to identify quantitative trait loci (QTLs) for domestication-related traits in yardlong bean and compare them with previously reported QTLs in closely related Vigna. Methods Two linkage maps were developed from BC1F1 and F2 populations from the cross between yardlong bean (V. unguiculata ssp. unguiculata cv.-gr. sesquipedalis) accession JP81610 and wild cowpea (V. unguiculata ssp. unguiculata var. spontanea) accession TVnu457. Using these linkage maps, QTLs for 24 domestication-related traits were analysed and mapped. QTLs were detected for traits related to seed, pod, stem and leaf. Key Results Most traits were controlled by between one and 11 QTLs. QTLs for domestication-related traits show co-location on several narrow genomic regions on almost all linkage groups (LGs), but especially on LGs 3, 7, 8 and 11. Major QTLs for sizes of seed, pod, stem and leaf were principally located on LG7. Pleiotropy or close linkage of genes for the traits is suggested in these chromosome regions. Conclusions This is the first report of QTLs for domestication-related traits in yardlong bean. The results provide a foundation for marker-assisted selection of domestication-related QTLs in yardlong bean and enhance understanding of domestication in the genus Vigna. PMID:22419763

Kongjaimun, Alisa; Kaga, Akito; Tomooka, Norihiko; Somta, Prakit; Vaughan, Duncan A.; Srinives, Peerasak

2012-01-01

213

A large plasmid isolated from Rhizobium huakuii bv. renge that includes genes for both nodulation of Astragalus sinicus cv. Japan and nitrogen fixation  

Microsoft Academic Search

Rhizobium huakuii bv. renge, which is able to produce nodules on Astragalus sinicus cv. Japan, has been assigned to a new biovariety based on its host range and taxonomic characteristics. We reproducibly demonstrated the presence of an indigenous large plasmid, with a molecular mass of about 260 Mda, in R. huakuii bv. renge and named the plasmid pRhYM. Using a

Yong Xu; Yoshikatsu Murooka

1995-01-01

214

A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV Hollot2, J Horowitz3, and RP Shrestha4, Y Chait5  

E-print Network

A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV on performance of anemia management protocols. · Anemia of end-stage kidney disease (ESRD) is characterized-specific gains should play a role in their design. · The interaction of anemia management protocols (AMPs

Massachusetts at Amherst, University of

215

Seasonal ultrastructural alterations in the plasma membrane produced by slow freezing in cortical tissues of mulberry ( Morus bombyciz Koidz. cv. Goroji)  

Microsoft Academic Search

Seasonal alterations in the ultrastructure of the plasma membrane produced by slow freezing were examined in cortical parenchyma cells of mulberry twigs (Morus bombyciz Koidz. cv. Goroji) grown in northern Japan. In freezing-sensitive summer, freezing produced distinct aparticulate domains with accompanying inverted hexagonalII (HII) phase transitions in the plasma membrane. In autumn and spring, during cold acclimation and deacclimation, freezing

Scizo Fujikawa

1994-01-01

216

Effect of various Rhizobium trifolii strains on nitrogenase (C 2 H 2 ) activity profiles of red clover ( Trifolium pratense cv. Venla)  

Microsoft Academic Search

The effect of variousRhizobium trifolii strains on the nitrogenase activity profiles of red clover (Trifolium pratense cv. Venla) was studied in greenhouse and field experiments. The nitrogenase activity of nodulated roots or whole plants was measured with the acetylene reduction assay at different stages of plant growth. The mean nitrogenase activity followed plant shoot growth rate from the time when

Kristina Lindström

1984-01-01

217

Effect of root infection with Pseudomonas fluorescens and Glomus mosseae on severity of lime?induced chlorosis in Vitis vinifera L. cv. ‘pinot blanc’  

Microsoft Academic Search

In order to achieve different degrees of tolerance to lime?induced chlorosis, Vitis vinifera L. cv. ‘Pinot blanc’ was grafted on two hybrid rootstocks: Vitis Berlandieri Planch, x Vitis rupestris Scheele ‘140 Ru’ (tolerant) and Vitis riparia Michx. x Vitis rupestris Scheele ‘101–14’ (susceptible). The vines were grown in pots with an unsterile calcareous soil, and roots infected with a suspension

L. Bavaresco; C. Fogher

1996-01-01

218

The Hewlett-Packard HP-41CV Hand-Held Computer as a Medium for Teaching Mathematics to Fire Control Systems Repairers. Research Report 1408.  

ERIC Educational Resources Information Center

A study compared the benefits of using the Hewlett-Packard HP-41CV hand-held computer, as opposed to conventional training without computers, in teaching mathematics to fire control systems repairers. Thirty soldiers in a course to train fire control systems repairers received training in technical mathematics using the hand-held computer, whereas…

Boldovici, John A.; Scott, Thomas D.

219

Draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi).  

PubMed

Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

Kolton, Max; Green, Stefan J; Harel, Yael Meller; Sela, Noa; Elad, Yigal; Cytryn, Eddie

2012-10-01

220

Arbuscular mycorrhizal fungi enhance fruit growth and quality of chile ancho ( Capsicum annuum L. cv San Luis) plants exposed to drought  

Microsoft Academic Search

The effect of arbuscular mycorrhizal fungi (AMF) and drought on fruit quality was evaluated in chile ancho (Capsicum annuum L. cv San Luis). AMF treatments were (1) Glomus fasciculatum (AMFG), (2) a fungal species consortium from the forest “Los Tuxtla” in Mexico (AMFT), (3) a fungal species consortium from the Sonorian desert in Mexico (AMFD), and (4) a noninoculated control

Hortencia G. Mena-Violante; Omar Ocampo-Jiménez; Luc Dendooven; Gerardo Martínez-Soto; Jaquelina González-Castañeda; Fred T. Davies Jr; Víctor Olalde-Portugal

2006-01-01

221

USE OF REPEATED BRONCHOALVEOLAR LAVAGE IN RABBITS TO ASSESS POLLUTANT-INDUCED LUNG CHANGES IN AN ANIMAL MODEL OF CARDIOVASCULAR (CV) DISEASE.  

EPA Science Inventory

Animal models of coronary heart disease (e.g., hyperlipidemic rabbits) are being used to investigate epidemiologic associations between higher levels of air pollution and adverse CV consequences. Mechanisms by which pollutant-induced lung or systemic inflammation leads to acute C...

222

Effect of potassium on drought resistance of Hibiscus rosa-sinensis cv. Leprechaun: Plant growth, leaf macro- and micronutrient content and root longevity  

Microsoft Academic Search

As competition for the limited water supply available for irrigation of horticultural crops increases, research into crop management practices that enhance drought resistance, plant water-use efficiency and plant growth when water supply is limited has become increasingly essential. This experiment was conducted to determine the effect of potassium (K) nutritional status on the drought resistance of Hibiscus rosa-sinensis L. cv.

Jonathan N. Egilla; Fred T. Davies; Malcolm C. Drew

2001-01-01

223

Nickolas Themelis: Director, Earth Engineering Center http://www.seas.columbia.edu/earth/szekielda-cv.html[8/19/2009 4:40:26 PM  

E-print Network

Nickolas Themelis: Director, Earth Engineering Center http://www.seas.columbia.edu/earth/szekielda-cv.html[8/19/2009 4:40:26 PM] The Earth Engineering Center of Columbia University KARL SZEKIELDA (212) 923 University of New York. RESEARCH INTERESTS Remote sensing and satellite imaging. Use of remote sensing

224

Effects of pH, sample size, and solvent partitioning on recovery of soluble phenolic acids and isoflavonoids in red clover (Trifolium pratense cv. Kenland)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Several extraction parameters were tested to determine optimal conditions for extracting phenolics from red clover (Trifolium pratense L. cv. Kenland). HPLC-UV profiles were compared before and after partitioning a methanolic extract of soluble phenolics with ethyl acetate-ether (1:1, v/v). The e...

225

Draft Genome Sequence of Flavobacterium sp. Strain F52, Isolated from the Rhizosphere of Bell Pepper (Capsicum annuum L. cv. Maccabi)  

PubMed Central

Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

Kolton, Max; Green, Stefan J.; Harel, Yael Meller; Sela, Noa; Elad, Yigal

2012-01-01

226

Efectos de extractos acuosos de la maleza Cyperus rotundus L. (Cyperaceae) sobre la germinación de semillas y crecimiento de plántulas de maíz (Zea mays L.) cv. Pioneer 3031  

Microsoft Academic Search

The objective of this experiment was to determine the effects of foliage water extracts of purple nutsedge (Cyperus rotundus L.) on seed germination and seedling growth of corn ( Cyperus rotundus L.) on seed germination and seedling growth of corn ( Cyperus rotundus Zea mays L.) cv. Pioneer 3031. An extract was prepared with nutsedge foliage of 15 days at

José A. Laynez-Garsaball; Jesús Rafael Méndez-Natera

227

CV -Teodoro Stadler, 2013 Teodoro Stadler -CV  

E-print Network

of a total project budget of US$ 8,213,170.- Design and conduct research on different aspects of boll weevil monitoring field-test for boll weevil populations (Patent) Organize updating international workshops) Neuquen province, Argentina. 1995-2000 Project Manager "Integrated Pest Management of the Cotton Boll

Lawrence, Rick L.

228

Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability  

PubMed Central

Background Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation. Reviewer This article was reviewed by Sándor Pongor, L. Aravind and Anthony M. Poole. PMID:23324115

2013-01-01

229

Long-Term Fungal Inhibitory Activity of Water-Soluble Extracts of Phaseolus vulgaris cv. Pinto and Sourdough Lactic Acid Bacteria during Bread Storage?  

PubMed Central

The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts of L. brevis AM7 sourdough and P. vulgaris cv. Pinto had a MIC of 40 mg of peptide/ml and 30.9 mg of protein/ml, respectively. MICs were markedly lower when chemically synthesized peptides or partially purified protein fractions were used. The water-soluble extract of P. vulgaris cv. Pinto showed inhibition toward a large number of fungal species isolated from bakeries. Phaseolin alpha-type precursor, phaseolin, and erythroagglutinating phytohemagglutinin precursor were identified in the water-soluble extract of P. vulgaris cv. Pinto by nano liquid chromatography-electrospray ionization-tandem mass spectrometry. When the antifungal activity was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all three proteins were inhibitory. A mixture of eight peptides was identified from the water-soluble extract of sourdough L. brevis AM7, and five of these exhibited inhibitory activity. Bread was made at the pilot plant scale by sourdough fermentation with L. brevis AM7 and addition of the water-soluble extract (27%, vol/wt; 5 mg of protein/ml) of P. vulgaris cv. Pinto. Slices of bread packed in polyethylene bags did not show contamination by fungi until at least 21 days of storage at room temperature, a level of protection comparable to that afforded by 0.3% (wt/wt) calcium propionate. PMID:18849463

Coda, Rossana; Rizzello, Carlo G.; Nigro, Franco; De Angelis, Maria; Arnault, Philip; Gobbetti, Marco

2008-01-01

230

Profiles of phenolic compounds and purine alkaloids during the development of seeds of Theobroma cacao cv. Trinitario.  

PubMed

Changes occurring in phenolic compounds and purine alkaloids, during the growth of seeds of cacao (Theobroma cacao) cv. Trinitario, were investigated using HPLC-MS/MS. Extracts of seeds with a fresh weight of 125, 700, 1550, and 2050 mg (stages 1-4, respectively) were analyzed. The phenolic compounds present in highest concentrations in developing and mature seeds (stages 3 and 4) were flavonols and flavan-3-ols. Flavan-3-ols existed as monomers of epicatechin and catechin and as procyanidins. Type B procyanidins were major components and varied from dimers to pentadecamer. Two anthocyanins, cyanidin-3-O-arabinoside and cyanidin-3-O-galactoside, along with the N-phenylpropernoyl-l-amino acids, N-caffeoyl-l-aspartate, N-coumaroyl-l-aspartate, N-coumaroyl-3-hydroxytyrosine (clovamide), and N-coumaroyltyrosine (deoxyclovamide), and the purine alkaloids theobromine and caffeine, were present in stage 3 and 4 seeds. Other purine alkaloids, such as theophylline and additional methylxanthines, did not occur in detectable quantities. Flavan-3-ols were the only components to accumulate in detectable quantities in young seeds at developmental stages 1 and 2. PMID:23215441

Pereira-Caro, Gema; Borges, Gina; Nagai, Chifumi; Jackson, Mel C; Yokota, Takao; Crozier, Alan; Ashihara, Hiroshi

2013-01-16

231

Effect of silver nanoparticles on rice (Oryza sativa L. cv. KDML 105) seed germination and seedling growth.  

PubMed

With the advances in nanotechnology, silver nanoparticles (AgNPs) have been applied in many industries, increasing their potential exposure level in the environment, yet their environmental safety remains poorly evaluated. The possible effects of different sized AgNPs (20, 30-60, 70-120 and 150nm diameter) on jasmine rice, Oryza sativa L. cv. KDML 105, were investigated at different concentrations (0.1, 1, 10, 100 and 1000mg/L) upon seed germination and seedling growth. The results revealed that the level of seed germination and subsequent growth of those seedlings that germinated were both decreased with increasing sizes and concentrations of AgNPs. Based on the analysis of AgNPs accumulation in plant tissues, it implied that the higher uptake was found when the seeds were treated with the smaller AgNPs, 20nm diameter AgNPs, but it was trapped in the roots rather than transported to the leaves. These resulted in the less negative effects on seedling growth, when compared to the seed soaking with the larger AgNPs with 150nm diameter. The negative effects of AgNPs were supported by leaf cell deformation when rice seeds were treated with 150-nm-diameter AgNP at the concentration of 10 or 100mg/L during seed germination. These results further strengthen our understanding of environmental safety information with respect to nanomaterials. PMID:24726943

Thuesombat, Pakvirun; Hannongbua, Supot; Akasit, Sanong; Chadchawan, Supachitra

2014-06-01

232

Influence of harvest date and crop yield on the fatty acid composition of virgin olive oils from cv. Picual.  

PubMed

In this study was analyzed the effect of crop year and harvesting time on the fatty acid composition of cv. Picual virgin olive oil. The study was carried out during the fruit ripening period for three crop seasons. The mean fatty acid composition of Picual oils was determined. The oils contained palmitic acid (11.9%), oleic acid (79.3%), and linoleic acid (2.95%). The content of palmitic acid and saturated fatty acids decreased during fruit ripening while oleic and linoleic acids increased. The amount of stearic and linolenic acids decreased. The amount of saturated acids, palmitic and stearic, and the polyunsaturated acids linoleic and linolenic was dependent on the time of harvest, whereas the amount of oleic acid varied with the crop year. The differences observed between crop years for both palmitic and linoleic acid may be explained by the differences in the temperature during oil biosynthesis and by the amount of summer rainfall for oleic acid content. A significant relationship was observed between the MUFA/PUFA ratio and the oxidative stability measured by the Rancimat method. PMID:15161211

Beltrán, Gabriel; Del Rio, Carmen; Sánchez, Sebastián; Martínez, Leopoldo

2004-06-01

233

Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening  

PubMed Central

Background Cluster thinning is an agronomic practice in which a proportion of berry clusters are removed from the vine to increase the source/sink ratio and improve the quality of the remaining berries. Until now no transcriptomic data have been reported describing the mechanisms that underlie the agronomic and biochemical effects of thinning. Results We profiled the transcriptome of Vitis vinifera cv. Sangiovese berries before and after thinning at veraison using a genome-wide microarray representing all grapevine genes listed in the latest V1 gene prediction. Thinning increased the source/sink ratio from 0.6 to 1.2 m2 leaf area per kg of berries and boosted the sugar and anthocyanin content at harvest. Extensive transcriptome remodeling was observed in thinned vines 2 weeks after thinning and at ripening. This included the enhanced modulation of genes that are normally regulated during berry development and the induction of a large set of genes that are not usually expressed. Conclusion Cluster thinning has a profound effect on several important cellular processes and metabolic pathways including carbohydrate metabolism and the synthesis and transport of secondary products. The integrated agronomic, biochemical and transcriptomic data revealed that the positive impact of cluster thinning on final berry composition reflects a much more complex outcome than simply enhancing the normal ripening process. PMID:22192855

2011-01-01

234

Early inhibition of photosynthesis during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14  

SciTech Connect

Early physiological effects of developing Mn toxicity in young leaves of burley tobacco (Nicotiana tabacum L. cv KY 14) were examined in glass-house/water cultured plants grown at high (summer) and low (winter) photon flux. Following transfer of plants to solutions containing 1 millimolar Mn{sup 2+}, sequential samplings were made at various times for the following 9 days, during which Mn accumulation by leaves increased rapidly from {approx} 70 on day 0 to {approx} 1700 and {approx} 5000 microgram per gram dry matter after 1 and 9 days, respectively. In plants grown at high photon flux, net photosynthesis declined by {approx} 20 and {approx} 60% after 1 and 9 days, respectively, and the onset of this decline preceded appearance (after 3 to 4 days) of visible foliar symptoms of Mn toxicity. Intercellular CO{sub 2} concentrations and rates of transpiration were not significantly affected. Though the activity of latent or activated polyphenol oxidase increased in parallel with Mn accumulation, neither leaf respiration nor the activity of catalase (EC 1.11.1.6) and peroxidase (EC 1.10.1.7) were greatly affected. These effects from Mn toxicity could not be explained by any changes in protein or chlorophyll abundance. Additionally, they were not a consequence of Mn induced Fe deficiency. Therefore, inhibition of net photosynthesis and enhancement of polyphenol oxidase activity are early indicators of excess Mn accumulation in tobacco leaves.

Nable, R.O.; Houtz, R.L.; Cheniae, G.M. (Univ. of Kentucky, Lexington (USA))

1988-04-01

235

Characterisation of extra virgin olive oils from Galician autochthonous varieties and their co-crushings with Arbequina and Picual cv.  

PubMed

The current trend of the olive oil market is the production of high quality extra from traditional minor olive varieties with peculiar and differentiated characteristics (especially with respect to the aromatic and phenolic composition). In this way, the interest of Galician oil producers (NW Spain) in recovering old autochthonous Local olive fruits has increased substantially in recent years. In order to investigate the potential of the Local olives by either producing high quality monovarietal oils or mixing with the most widespread olives in Galicia (Arbequina and Picual cv.), quality indices, and fatty acid composition as well as volatile and phenolic profiles were determined and compared. All EVOOs studied in this work can be considered as "extra virgin olive oil" due to quality indices fell within the ranges established in legislation. Picual and Local olive oils as well as those resulting from their co-crushing reach values which are required by EU legislation to add the specific health claim on the oil label. Co-crushing Picual:Local (80:20) provided a significant enhancement of grass and apple nuances and a decrease of banana notes with respect to Picual oils. The co-crushing process improved sensory and health properties of Picual extra virgin olive oils. The effect of co-crushing on phenolics, ester volatiles and banana nuances cannot be easily modulated, contrary to quality indices and fatty acid composition, both changing linearly in strict correlation with the fruit mass ratio. PMID:25624261

Reboredo-Rodríguez, P; González-Barreiro, C; Cancho-Grande, B; Fregapane, G; Salvador, M D; Simal-Gándara, J

2015-06-01

236

Effect of gamma rays on different explants of callus treatment of multiple shoots in Cucumis melo cv. Bathasa.  

PubMed

A mutagenesis programme was carried out using physical mutagens (gamma rays) on Cucumis melo cv. Bathasa. In irradiated seeds the number of shoots formed in the lower doses was significantly higher than the controls. Decrease in the number of shoots and shoot bud formation was observed with higher doses in all the explants studies. There was complete lethality in the 10, 15 and 20 kR doses. In irradiated stem cultures the maximum number of shoots were observed in 2 kR. The number of shoots decreased with increasing doses of irradiation. At higher doses of 4 and 5kR, light green compact callus was formed in almost all the explants. The effect of lower doses of gamma irradiation on shoot bud formation and rooting efficiency from nodal explants cultured on MS +2.0 mgl(-1) L-glutamic acid + 0.5 mgl(-1) BAP, stimulation of shoot and root induction were studied. The number of shoots and root lengths decreased with the increasing dosage of irradiation. The irradiated callus was grown on solidified MS medium with containing 2.0 mgl(-1) BAP + 1.0 mgl(-1) IAA. There was a significant stimulation of growth in the callus at lower doses. At higher doses like 15 and 20 kRs growth was drastically reduced. PMID:19295084

Venkateshwarlu, M

2008-09-01

237

Quantification of Apoplastic Potassium Content by Elution Analysis of Leaf Lamina Tissue from Pea (Pisum sativum L. cv Argenteum) 1  

PubMed Central

K+ content and concentration within the apoplast of mesophyll tissue of pea (Pisum sativum L., cv Argenteum) leaflets were determined using an elution procedure. Following removal of the epidermis, a 1 centimeter (inside diameter) glass cylinder was attached to the exposed mesophyll tissue and filled with 5 millimolar CaCl2 solution (1°C). From time-course curves of cumulative K+ diffusion from the tissue, the amount of K+ of extracellular origin was estimated. Apoplastic K+ contents for leaves from plants cultured in nutrient solution containing 2 or 10 millimolar K+ were found to range from 1 to 4.5 micromoles per gram fresh weight, comprising less than 3% of the total K+ content within the lamina tissue. Assuming an apoplastic solution volume of 0.04 to 0.1 milliliters per gram fresh weight and a Donnan cation exchange capacity of 2.63 micromoles per gram fresh weight (experimentally determined), the K+ concentration within apoplastic solution was estimated at 2.4 to 11.8 millimolar. Net movement of Rb+ label from the extracellular compartment within mesophyll tissue into the symplast was demonstrated by pulse-chase experiments. It was concluded that the mesophyll apoplast in pea has a relatively low capacitance as an ion reservoir. Apoplastic K+ content was found to be highly sensitive to changes in xylem solution concentration. Images Figure 1 PMID:16667794

Long, Jean M.; Widders, Irvin E.

1990-01-01

238

Spectral variability of /s/ in sV and sCV sequences produced by adults and children  

NASA Astrophysics Data System (ADS)

Previous research has demonstrated that both children and adults produce /s/ with greater spectral variability in /sp/ sequences than /st/ sequences, when these sequences are embedded in the medial position of CVCCVC nonwords [B. Munson, J. Acoust. Soc. Am. 110, 1203-1206 (2001)]. The current study examined whether this result could be replicated when /s/ is embedded in syllable-onset clusters, with a variety of following consonants and vowels. Adults and children aged 3-7 were recorded producing multiple tokens of sV and sCV nonwords, where the vowel was either /i/, /opena/, or /u/, and the consonant was either /p/, /t/, /w/, or /l/. For each token, the spectral mean of non-overlapping 10-ms windows of frication noise was calculated. Nonlinear regressions of the form y=aebx were used to predict the spectral mean of each portion of frication noise from its position in the fricative. The resulting measure of model fit, R2, was used as an index of within-speaker variability. For each participant, separate R2 values were calculated for /s/ in each of the 15 phonetic contexts. Analyses will address the influence of age, consonant context, and vowel context on spectral variability.

Munson, Benjamin

2002-05-01

239

Characterization of the adaptive response of grapevine (cv. Tempranillo) to UV-B radiation under water deficit conditions.  

PubMed

This work aims to characterize the physiological response of grapevine (Vitis vinifera L.) cv. Tempranillo to UV-B radiation under water deficit conditions. Grapevine fruit-bearing cuttings were exposed to three levels of supplemental biologically effective UV-B radiation (0, 5.98 and 9.66kJm(-2)day(-1)) and two water regimes (well watered and water deficit), in a factorial design, from fruit-set to maturity under glasshouse-controlled conditions. UV-B induced a transient decrease in net photosynthesis (Anet), actual and maximum potential efficiency of photosystem II, particularly on well watered plants. Methanol extractable UV-B absorbing compounds (MEUVAC) concentration and superoxide dismutase activity increased with UV-B. Water deficit effected decrease in Anet and stomatal conductance, and did not change non-photochemical quenching and the de-epoxidation state of xanthophylls, dark respiration and photorespiration being alternative ways to dissipate the excess of energy. Little interactive effects between UV-B and drought were detected on photosynthesis performance, where the impact of UV-B was overshadowed by the effects of water deficit. Grape berry ripening was strongly delayed when UV-B and water deficit were applied in combination. In summary, deficit irrigation did not modify the adaptive response of grapevine to UV-B, through the accumulation of MEUVAC. However, combined treatments caused additive effects on berry ripening. PMID:25617319

Martínez-Lüscher, J; Morales, F; Delrot, S; Sánchez-Díaz, M; Gomès, E; Aguirreolea, J; Pascual, I

2015-03-01

240

In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH-B1 through enhanced axillary branching.  

PubMed

Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10-15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 ?M + IAA 5.7 ?M. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 ?M + IAA 5.7 ?M. The micro shoots were rooted (100 %) on + IAA 5.7 ?M. In vitro rooted plants were acclimatized on autoclaved coconut husk containing plant salt mixture and under shade net house (50 % shade 70-80 % RH). The plants were established in the field after acclimatization. The micropropagated plants were tested for its genetic fidelity using 13 RAPD, 3 ISSR and 2 DAMD primers. Profile obtained by all the three Single Primer Amplification Reaction (SPAR) technique from mother tree and micropropagated plants revealed genetic integrity of micropropagated plants with that of mother tree. PMID:23572900

Pati, Rajesh; Chandra, Ramesh; Chauhan, Ugam Kumari; Mishra, Maneesh; Srivastava, Navin

2008-10-01

241

Forsterite-bearing type B refractory inclusions from CV3 chondrites: From aggregates to volatilized melt droplets  

NASA Astrophysics Data System (ADS)

Detailed petrologic and oxygen isotopic analysis of six forsterite-bearing Type B calcium-aluminum-rich inclusions (FoBs) from CV3 chondrites indicates that they formed by varying degrees of melting of primitive precursor material that resembled amoeboid olivine aggregates. A continuous evolutionary sequence exists between those objects that experienced only slight partial melting or sintering through objects that underwent prolonged melting episodes. In most cases, melting was accompanied by surface evaporative loss of magnesium and silicon. This loss resulted in outer margins that are very different in composition from the cores, so much so that in some cases, the mantles contain mineral assemblages that are petrologically incompatible with those in the cores. The precursor objects for these FoBs had a range of bulk compositions and must therefore have formed under varying conditions if they condensed from a solar composition gas. Five of the six objects show small degrees of mass-dependent oxygen isotopic fractionation in pyroxene, spinel, and olivine, consistent with the inferred melt evaporation, but there are no consistent differences among the three phases. Forsterite, spinel, and pyroxene are 16O-rich with ?17O ˜ -24‰ in all FoBs. Melilite and anorthite show a range of ?17O from -17‰ to -1‰.

Bullock, Emma S.; MacPherson, Glenn J.; Nagashima, Kazuhide; Krot, Alexander N.; Petaev, Michail I.; Jacobsen, Stein B.; Ulyanov, Alexander A.

2012-12-01

242

Noble Gas Analysis of the Julesburg L3.6, Tulia H4, Y-86789 Thermally Metamorphosed CM, and Allende CV3 Chondrites by Crushing Method  

Microsoft Academic Search

We analyzed noble gases of the Julesburg L3.6, Tulia H4, Y-86789 thermally metamorphosed CM, and Allende CV3 chondrites by the stepped crushing method. All samples have released light noble gases with meteoritic isotopic compositions and heavy noble gases which are contaminated with air to various extents. Total amounts of crash-released noble gases of the four meteorites are estimated to be

D. Fukuda; T. Nakamura; N. Takaoka; K. Nagao

1996-01-01

243

Photosynthetic characteristics and growth responses of dwarf apple ( Malus domesti ca Borkh. cv. Fuji) saplings after 3 years of exposure to elevated atmospheric carbon dioxide concentration and temperature  

Microsoft Academic Search

Growth and photosynthetic responses of dwarf apple saplings (Malus domestica Borkh. cv. Fuji) acclimated to 3 years of exposure to contrasting atmospheric CO2 concentrations (360 and 650 µmol mol-1) in combination with current ambient or elevated (ambient +5°C) temperature patterns were determined. Four 1-year-old apple saplings grafted onto M.9 rootstocks were each enclosed in late fall 1997 in a controlled

Hee-Myong Ro; Pan-Gi Kim; In-Bog Lee; Myoung-Soon Yiem; Su-Young Woo

2001-01-01

244

Petrography, stable isotope compositions, microRaman spectroscopy, and presolar components of Roberts Massif 04133: A reduced CV3 carbonaceous chondrite  

NASA Astrophysics Data System (ADS)

Here, we report the mineralogy, petrography, C-N-O-stable isotope compositions, degree of disorder of organic matter, and abundances of presolar components of the chondrite Roberts Massif (RBT) 04133 using a coordinated, multitechnique approach. The results of this study are inconsistent with its initial classification as a Renazzo-like carbonaceous chondrite, and strongly support RBT 04133 being a brecciated, reduced petrologic type >3.3 Vigarano-like carbonaceous (CV) chondrite. RBT 04133 shows no evidence for aqueous alteration. However, it is mildly thermally altered (up to approximately 440 °C); which is apparent in its whole-rock C and N isotopic compositions, the degree of disorder of C in insoluble organic matter, low presolar grain abundances, minor element compositions of Fe,Ni metal, chromite compositions and morphologies, and the presence of unequilibrated silicates. Sulfides within type I chondrules from RBT 04133 appear to be pre-accretionary (i.e., did not form via aqueous alteration), providing further evidence that some sulfide minerals formed prior to accretion of the CV chondrite parent body. The thin section studied contains two reduced CV3 lithologies, one of which appears to be more thermally metamorphosed, indicating that RBT 04133, like several other CV chondrites, is a breccia and thus experienced impact processing. Linear foliation of chondrules was not observed implying that RBT 04133 did not experience high velocity impacts that could lead to extensive thermal metamorphism. Presolar silicates are still present in RBT 04133, although presolar SiC grain abundances are very low, indicating that the progressive destruction or modification of presolar SiC grains begins before presolar silicate grains are completely unidentifiable.

Davidson, Jemma; Schrader, Devin L.; Alexander, Conel M. O'd.; Lauretta, Dante S.; Busemann, Henner; Franchi, Ian A.; Greenwood, Richard C.; Connolly, Harold C.; Domanik, Kenneth J.; Verchovsky, Alexander

2014-12-01

245

Effect of Intranasal Administration of CV11974, a Type 1 Angiotensin II Receptor Antagonist, on Airway Hyperresponsiveness and Airway Inflammation Induced by Antigen Inhalation in Guinea Pigs  

Microsoft Academic Search

Background: Angiotensin II is a putative mediator in asthma, but the effect of topical administration of type 1 angiotensin II (AT1) receptor antagonists on allergic airway reactions is not known. Objective: To investigate the effect of intranasal administration of CV-11974, an AT1 receptor antagonist, and of PD123319, a type 2 angiotensin II (AT2) receptor antagonist, on antigen-induced airway reactions in

Shigeharu Myou; Masaki Fujimura; Toshiyuki Kita; Kazuyoshi Watanabe; Tatsuki Hirose; Hideki Tachibana; Yoshihisa Ishiura; Shinji Nakao

2002-01-01

246

Analysis of the Expression of Anthocyanin Pathway Genes in Developing Vitis vinifera 1. cv Shiraz Grape Berries and the - Implications for Pathway Regdation  

Microsoft Academic Search

Anthocyanin synthesis in Vitis vinifera L. cv Shiraz grape berries began 1 O weeks postflowering and continued throughout berry ripening. Expression of seven genes of the anthocyanin biosynthetic pathway (phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone-3-hydroxylase (F3Hl, dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygen- ase (LDOX), and UDP glucose-flavonoid 3-o-glucosyl transferase (UFCT)) was determined. In flowers and grape

Paul K. BOSS; Christopher Davies; Simon P. Robinson

247

The lipid composition, fluidity, and Mg 2+ ATPase activity of rice ( Oryza sativa L. cv. Bahia) shoot plasma membranes: effects of ABA and GA 3  

Microsoft Academic Search

Six-day-old rice plants (Oryza sativa L., cv Bahia) were grown for 5 days more in nutrient solution culture containing 10?5 M abscisic acid (ABA) or gibberellic acid (GA3) (treated plants). Plasma membrane (PM) vesicles were isolated from the shoots of treated or control plants, and ATPase hydrolytic\\u000a and proton-pumping activity, fluidity, and free sterol and phospholipid composition were determined. Both

C. Martínez-Cortina; R. Ros; D. T. Cooke; C. S. James; A. Sanz

1992-01-01

248

Long-term treatment with angiotensin II type 1 receptor antagonist, CV-11974, restores beta-catenin mRNA expression in volume-overloaded rabbit hearts.  

PubMed

This study aimed to search genes altered in expression after long-term treatment with the angiotensin II type 1 receptor (AT1) antagonist, CV-11974, in volume-overloaded hearts. Arteriovenous shunt was made between the common carotid artery and jugular vein in Japanese White rabbits. Shunt-operated rabbits were randomly treated with CV-11974 or vehicle for 6 weeks, starting 6 weeks after surgery. As controls, sham-operated rabbits were given vehicle. Total RNA was prepared from each left ventricular myocardium. Using differential display, we screened one cDNA encoding human beta-catenin, in which the expression was upregulated after CV-11974 administration in shunt rabbit hearts. Beta-catenin is a molecule that exists in the intercalated disks and also works in cytoplasm as a major component of Wnt signaling. We then examined mRNA expressions of beta-catenin and connexin43 by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The mRNA expressions of beta-catenin and connexin43 were markedly depressed in shunt-operated animals given vehicle compared with sham-operated animals (P < 0.01). These results suggest that downregulation of beta-catenin and connexin43 expression might be involved in the process of ventricular remodeling by volume overload. The RT-PCR also demonstrated that beta-catenin mRNA expression was significantly higher in shunt rabbits treated with CV-11974 than in those given vehicle (P < 0.05). These data suggest that volume overload may downregulate beta-catenin expression by an AT1 receptor-mediated pathway. PMID:12434200

Itoh, Makoto; Takeishi, Yasuchika; Nakada, Shigekazu; Miyamoto, Takuya; Tsunoda, Yuichi; Takahashi, Hiroki; Kubota, Isao; Tomoike, Hitonobu

2002-11-01

249

Application of silver nanoparticles contained in ethanol as a working fluid in an oscillating heat pipe with a check valve (CLOHP/CV): a thermodynamic behaviour study  

NASA Astrophysics Data System (ADS)

This research focused on the thermal behaviour of oscillating heat pipe with a check valve (CLOHP/CV). The CLOHP/CV was made from a copper capillary tube with an internal diameter of 2.03 mm, 40 turns, and 2 check valves. The CLOHP/CV had three sections: the evaporator and adiabatic condenser of a length of 50, 100, and 150 mm. The angles of inclinations were 90°, 80°, 60°, 40°, 20° and 0° at normal operating conditions. The two working fluids were an ethanol and silver nanoparticles concentration of 0.5 wt% contained in the ethanol (NE). The filling ratio was 50 % with respect to the total volume of the tube. The operating temperature (T w ) was 45, 65, 85, 105, 125 and 150 °C. The results of the study showed that the heat flux increased significantly when the operating temperature increased, and the heat flux increased when the L e decreased from 150 to 50 mm. Furthermore, the thermal behaviour of CLOHP/CV showed the highest heat flux of 2,012.63 W/m2 at L e of 50, with an angle of inclination of 90° and operating temperature of 150 °C with NE. The behaviour of 0.5 wt% of silver nanoparticles contained in the ethanol produced a good contact angle. The 0.5 wt% of silver nanoparticles decreased the wet ability, thus increasing the thermal behaviour. The optimum concentration for the addition of silver nanoparticles in the working fluid was 0.5 wt%.

Bhuwakietkumjohn, N.; Parametthanuwat, T.

2015-01-01

250

Long-Term Fungal Inhibitory Activity of Water-Soluble Extracts of Phaseolus vulgaris cv. Pinto and Sourdough Lactic Acid Bacteria during Bread Storage  

Microsoft Academic Search

The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts

Rossana Coda; Carlo G. Rizzello; Franco Nigro; Maria De Angelis; Philip Arnault; Marco Gobbetti

2008-01-01

251

Does nitrogen supply affect the response of wheat (Triticum aestivum cv. Hanno) to the combination of elevated CO2 and O3?  

Microsoft Academic Search

Spring wheat (Triticum aestivum cv. Hanno) was grown at ambient (350 mmol mol? 1) or elevated CO2 (700 mmol mol? 1) in charcoaluPurafil1-filtered air (CFA - 5n mol mol ? 1) or ozone (CFA q75 nmol mol? 1 7h d? 1) at three levels of N supply (1.5, 4 and 14 mM NO?3 ), to test the hypothesis that the

Joao Cardoso-Vilhena; Jeremy Barnes

2001-01-01

252

Detection and discrimination of two fungal diseases of mango (cv. Keitt) fruits based on volatile metabolite profiles using GC\\/MS  

Microsoft Academic Search

Volatile organic compounds collected from the headspace of mango cv. Keitt inoculated with Lasiodiplodia theobromae (stem-end rot), Colletotrichum gloeosporioides (anthracnose), mock (as the first control) and non-wounded-non-inoculated mango (as the second control) were analyzed using GC\\/MS to investigate the feasibility of automatic detection and diagnosis of diseases of mango in stores. A total of 37 metabolites, relatively consistent in 8

M. Moalemiyan; A. Vikram; A. C. Kushalappa

2007-01-01

253

Light-induced root hair formation in lettuce ( Lactuca sativa L. cv. Grand Rapids) roots at low pH is brought by chlorogenic acid synthesis and sugar  

Microsoft Academic Search

Previously, we reported that chlorogenic acid (CGA) facilitated root hair formation at pH 4.0 in lettuce (Lactuca sativa L. cv. Grand Rapids). Light was essential for this process. In the present study, we determined relationships between CGA,\\u000a light, and sugar during root hair formation in lettuce seedlings. The amount of CGA increased with white light in intact seedlings.\\u000a Exogenously applied

Megumi Narukawa; Keiji Watanabe; Yasunori Inoue

2010-01-01

254

The effect of supplementation of root zone dissolved inorganic carbon on fruit yield and quality of tomatoes (cv ‘Daniella’) grown with salinity  

Microsoft Academic Search

The possibility that elevated root zone dissolved inorganic carbon (DIC) concentrations could increase yield and quality of Lycopersicon esculentum (L.) Mill. cv Daniella when combined with salinity in an agricultural production system was investigated. Root zone DIC was modified by supplying CO2-enriched irrigation solutions (1gl?1), NaHCO3-enriched irrigation solutions (10mM) or aerating the roots with ambient air or with air enriched

Michael D Cramer; Johannes A Oberholzer; Nicolaas J. J Combrink

2001-01-01

255

Aflatoxin — induced alteration in the levels of membrane chemicals of subcellular organelles isolated from excised, incubated Glycine max, cv. ‘Essex’ roots  

Microsoft Academic Search

Isolates of aflatoxin-producing strains of Aspergillus grow on autoclaved and field-grown (lesser extent) Glycine max beans. Both mixed and aflatoxin B1 inhibit G. max, cv. ‘Essex’ bean germination and elongation of either attached or excised cultured roots. Because B1 impairs the latter roots' ability to intracellularize [14C]-leucine, it may alter plasmalemma structure and\\/or function. To determine whether incubation of excised

J. M. Danley; S. Staggers; S. Walker; A. Varnert; G. C. Llewellyn; W. V. Dashek

1981-01-01

256

Physical Characterization of the Potentially Hazardous Asteroid and Planetary Radar Target 68216 (2001 CV26): An Excellent Shape/Pole Modeling Candidate.  

NASA Astrophysics Data System (ADS)

The Near-Earth Asteroid (NEA) 68216 (2001 CV26) was discovered February 1 2001 by the LINEAR NEA Discovery Survey (MPEC 2001-F15). With an Absolute Magnitude H=16.2 and a Minimum Orbit Intersection Distance MOID=0.023 AU, it has been flagged as a Potentially Hazardous Asteroid (PHA) by the Minor Planet Center. The asteroid's 2009-2010 apparition allowed for radar observation at both the Arecibo and Goldstone facilities in October 2009.

Hicks, M.; Lawrence, K.; Somers, J.; Foster, J.

2010-03-01

257

Physical Characterization of the Potentially Hazardous Asteroid and Planetary Radar Target 68216 (2001 CV26): An Excellent Shape\\/Pole Modeling Candidate  

Microsoft Academic Search

The Near-Earth Asteroid (NEA) 68216 (2001 CV26) was discovered February 1 2001 by the LINEAR NEA Discovery Survey (MPEC 2001-F15). With an Absolute Magnitude H=16.2 and a Minimum Orbit Intersection Distance MOID=0.023 AU, it has been flagged as a Potentially Hazardous Asteroid (PHA) by the Minor Planet Center. The asteroid's 2009-2010 apparition allowed for radar observation at both the Arecibo

M. Hicks; K. Lawrence; J. Somers; J. Foster

2010-01-01

258

Influence of technology, storage and exposure on components of extra virgin olive oil (Bosana cv) from whole and de-stoned fruits  

Microsoft Academic Search

We investigated the influence of technology, storage and exposure on quality parameters, minor components, oxidative stability and antioxidant activity in two extra virgin olive oils of Bosana cv, obtained from whole (WO) and de-stoned fruits (DO), processed with a two-phase decanter. DO oils showed great stability and, consequently, had a longer shelf-life than WO oils. During storage, the former maintained

A. Del Caro; V. Vacca; M. Poiana; P. Fenu; A. Piga

2006-01-01

259

Comparative analysis of the energy levels of planar and core-twisted perylene bisimides in solution and solid state by UV/VIS, CV, and UPS/IPES  

NASA Astrophysics Data System (ADS)

The frontier orbital energies of four different functionalized perylene bisimide derivatives, PBI-Ph(iPr)2, PBI-H4, PBI-F2 and PBI-Cl4, were directly determined by UV-photo electron spectroscopy (UPS) and inverse photo electron spectroscopy (IPES) and are compared to the results from cyclic voltammetry (CV) and optical absorption spectroscopy (UV/VIS). The optical spectra reveal significant differences between monomeric species in solution and assembled molecules in the condensed state for the nearly planar PBI-H4 and PBI-F2, which are attributed to significant ?- ? stacking interactions in the condensed phase. In contrast, for PBIs with bulky substituents or twisted core, i.e. 2,6-isopropylphenyl substituents at the imide positions (PBI-Ph(iPr)2) or four chlorine substituents at perylene bay positions (PBI-Cl4), similar spectra are observed in solution and in the condensed state, which suggests the absence of strong intermolecular ?- ? stacking interactions. An entirely different result is obtained for the HOMO/LUMO energy values obtained from UPS/IPES and CV measurements which do not reveal a significant impact of intermolecular ?- ? stacking interactions. When comparing CV and UPS/IPES results, an accentuated deviation was observed for the perylene bisimide derivatives as compared to correlations found in the literature.

Arantes, C.; Scholz, M.; Schmidt, R.; Dehm, V.; Rocco, M. L. M.; Schöll, A.; Reinert, F.; Würthner, F.

2012-09-01

260

Hydrothermal origin of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A CAI from the Northwest Africa 2086 CV3 chondrite  

NASA Astrophysics Data System (ADS)

We report an occurrence of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A calcium-aluminum-rich inclusion (CAI) from the CV3 (Vigarano-like) carbonaceous chondrite Northwest Africa 2086. Dmisteinbergite occurs as approximately 10 ?m long and few micrometer-thick lath-shaped crystal aggregates in altered parts of the CAI, and is associated with secondary nepheline, sodalite, Ti-poor Al-diopside, grossular, and Fe-rich spinel. Spinel is the only primary CAI mineral that retained its original O-isotope composition (?17O ~ -24‰); ?17O values of melilite, perovskite, and Al,Ti-diopside range from -3 to -11‰, suggesting postcrystallization isotope exchange. Dmisteinbergite, anorthite, Ti-poor Al-diopside, and ferroan olivine have 16O-poor compositions (?17O ~ -3‰). We infer that dmisteinbergite, together with the other secondary minerals, formed by replacement of melilite as a result of fluid-assisted thermal metamorphism experienced by the CV chondrite parent asteroid. Based on the textural appearance of dmisteinbergite in NWA 2086 and petrographic observations of altered CAIs from the Allende meteorite, we suggest that dmisteinbergite is a common secondary mineral in CAIs from the oxidized Allende-like CV3 chondrites that has been previously misidentified as a secondary anorthite.

Fintor, Krisztian; Park, Changkun; Nagy, Szabolcs; Pál-Molnár, Elemér; Krot, Alexander N.

2014-05-01

261

Colonization and nitrogenase activity of Triticum aestivum (cv. Baccross and Mahdavi) to the dual inoculation with Azospirillum brasilense and Rhizobium meliloti plus 2,4-D.  

PubMed

The potential enhancement of root colonization and nitrogenase activity of wheat cultivars (Baccross and Mahdavi) was studied with application of two Azospirillum brasilense strains (native and Sp7) co-inoculated with two Rhizobium meliloti strains (native and DSMZ 30135). The results indicated that the colonization was different due to the strains and cultivars of wheat were used. Native A. brasilense colonized wheat root better than Sp7 strain. However, Baccross cv. reacted better with native Azospirillum compared to Mahdavi cv. which reacted better with Sp7. When plants inoculated with dual inoculants (SP7 with standard Rhizobium), the colonization of Azospirillum were increased significantly (from 1.67 x 10(5) to 22 x 10(5) cfu g(-1) FW for Baccras cv. and 3.67 x 10(5) to 26 x 10(5) cfu g(-1) FW for Mahdavi cultivar). When the standard Rhizobium as co-inoculants changed to the native Rhizobium, the colonization of Azospirillum was higher when compared to the single inoculants but was almost the same when compared to the standard Rhizobium. When the standard or native strains of Rhizobium used as single inoculation of wheat roots, the number of Rhizobium in the wheat roots were not changed significantly. However, when plants co-inoculated with Rhizobium and Azospirillum, the colonization of Rhizobium was increased. Co-inoculation of standard strain of R. melilot with A. brasilense Sp7 showed that the colonization of Rhizobium were increased from 0.67 x 10(5) to 21 x 10(5) cfu g(-1) FW for Baccross cv. and 0.33 x 10(5) to 18 x 10(5) cfu g(-1) FW for Mahdavi cv. This behavior was the same when inoculation of Rhizobium was happened with the native one. In dual inoculation, the highest nitrogenase activity was measured in combination of the local strains (native A. brasilense with the native R. meliloti) and the lower one belongs to the combination of standard strains (Sp7 with standard R. meliloti). The difference in nirtogenase activity for different cultivars of wheat with Sp7 and standard Rhizobium is not significant but the difference for Sp7 strain plus native Rhizobium is significant (p > 0.05). However, the differences were not significant (p < 0.05) for nitrogenase activity in bacterial tubes, the difference for nitrogenase activity of co-inoculated plants with combination of Sp7 and Rhizobium either standard or native were significantly different. PMID:18819640

Mehry, Askary; Akbar, Mostajeran; Giti, Emtiazi

2008-06-15

262

S-GPBE: A Power-Efficient Broadcast Routing Algorithm Using Sectored Antenna Intae Kang and Radha Poovendran  

E-print Network

of directional (smart) or sector antenna to wireless adhoc (or sensor) networks to reduce the transmit power.g., horn antenna [6]) and one class of smart antennas called switched beam antenna [7]. While of smart antenna systems) is implic- itly assumed, because RB-BIP or D-BIP algorithms require an unlimited

Poovendran, Radha

263

An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor.  

PubMed

Apple flavor is characterized by combinations of ester compounds, which increase markedly during fruit ripening. The final step in ester biosynthesis is catalyzed by alcohol acyl transferases (AATs) that use coenzyme A (CoA) donors together with alcohol acceptors as substrates. The gene MpAAT1, which produces a predicted protein containing features of other plant acyl transferases, was isolated from Malus pumila (cv. Royal Gala). The MpAAT1 gene is expressed in leaves, flowers and fruit of apple. The recombinant enzyme can utilize a range of alcohol substrates from short to medium straight chain (C3-C10), branched chain, aromatic and terpene alcohols. The enzyme can also utilize a range of short to medium chain CoAs. The binding of the alcohol substrate is rate limiting compared with the binding of the CoA substrate. Among different alcohol substrates there is more variation in turnover compared with K(m) values. MpAAT1 is capable of producing many esters found in Royal Gala fruit, including hexyl esters, butyl acetate and 2-methylbutyl acetate. Of these, MpAAT1 prefers to produce the hexyl esters of C3, C6 and C8 CoAs. For the acetate esters, however, MpAAT1 preference depends upon substrate concentration. At low concentrations of alcohol substrate the enzyme prefers utilizing the 2-methylbutanol over hexanol and butanol, while at high concentrations of substrate hexanol can be used at a greater rate than 2-methylbutanol and butanol. Such kinetic characteristics of AATs may therefore be another important factor in understanding how the distinct flavor profiles of different fruit are produced during ripening. PMID:15955071

Souleyre, Edwige J F; Greenwood, David R; Friel, Ellen N; Karunairetnam, Sakuntala; Newcomb, Richard D

2005-06-01

264

Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)  

SciTech Connect

Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the plasma membrane, accounting for 16 mole % of the total lipid. Phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine with lesser amounts of phosphatidylglycerol, phosphatidic acid, phosphatidylserine, and phosphatidylinositol, comprised only 32 mole% of the total lipid in NA samples. Following cold acclimation, free sterols increased from 33 to 44 mole %, while steryl glucosides and acylated steryl glucosides decreased from 15 to 6 mole % and 4 to 1 mole %, respectively. Sterol analyses of these lipid classes demonstrated that free {beta}-sitosterol increased from 21 to 32 mole % (accounting for the increase in free sterols as a class) at the expense of sterol derivatives containing {beta}-sitosterol. Glucocerebrosides decreased from 16 to 7 mole % of the total lipid following cold acclimation. In addition, the relative proportions of associated hydroxy fatty acids, including 22:0 (h), 24:0 (h), 22:1 (h), and 24:1 (h) were altered. The phospholipid content of the plasma membrane fraction increased to 42 mole % of the total lipid following cold acclimation. Although the relative proportions of the individual phospholipids did not change appreciably after cold acclimation, there were substantial differences in the molecular species. Di-unsaturated molecular species of phosphatidylcholine and phosphatidylethanolamine increased following acclimation. These results demonstrate that cold acclimation results in substantial changes in the lipid composition of the plasma membrane.

Lynch, D.V.; Steponkus, P.L. (Cornell Univ., Ithaca, NY (USA))

1987-01-01

265

Root exudation and root development of lettuce (Lactuca sativa L. cv. Tizian) as affected by different soils.  

PubMed

Development and activity of plant roots exhibit high adaptive variability. Although it is well-documented, that physicochemical soil properties can strongly influence root morphology and root exudation, particularly under field conditions, a comparative assessment is complicated by the impact of additional factors, such as climate and cropping history. To overcome these limitations, in this study, field soils originating from an unique experimental plot system with three different soil types, which were stored at the same field site for 10 years and exposed to the same agricultural management practice, were used for an investigation on effects of soil type on root development and root exudation. Lettuce (Lactuca sativa L. cv. Tizian) was grown as a model plant under controlled environmental conditions in a minirhizotrone system equipped with root observation windows (rhizoboxes). Root exudates were collected by placing sorption filters onto the root surface followed by subsequent extraction and GC-MS profiling of the trapped compounds. Surprisingly, even in absence of external stress factors with known impact on root exudation, such as pH extremes, water and nutrient limitations/toxicities or soil structure effects (use of sieved soils), root growth characteristics (root length, fine root development) as well as profiles of root exudates were strongly influenced by the soil type used for plant cultivation. The results coincided well with differences in rhizosphere bacterial communities, detected in field-grown lettuce plants cultivated on the same soils (Schreiter et al., this issue). The findings suggest that the observed differences may be the result of plant interactions with the soil-specific microbiomes. PMID:24478764

Neumann, G; Bott, S; Ohler, M A; Mock, H-P; Lippmann, R; Grosch, R; Smalla, K

2014-01-01

266

Quantified, whole section trace element mapping of carbonaceous chondrites by Synchrotron X-ray Fluorescence Microscopy: 1. CV meteorites  

NASA Astrophysics Data System (ADS)

We present the application of a new synchrotron-based technique for rapid mapping of trace element distributions across large areas of the CV3 meteorites Allende and Vigarano. This technique utilizes the Australian Synchrotron X-ray Fluorescence Microscopy (XFM) beam line with its custom designed and built X-ray detector array called Maia. XFM with Maia allows data to be collected using a 2 ?m spot size at very low dwell times (?0.1-0.5 ms), resulting in maps of entire thin sections in ?5 h. Maia is an energy dispersive detector system with a large collection solid-angle, which allows full spectral acquisition and high sensitivity. Hence, there is no need to constrain the elements of interest a priori. We collected whole section maps (?2 cm × 1 cm) from 3 thick sections of Allende and a single map (2 cm × 1.5 cm) from a thick section of Vigarano. Our experimental conditions provide data for elements with 20 ? Z ? 40 (K-shell, Ca through Zr) and the L-emissions of Os, Ir, Pt, Au, and Pb. We illustrate the unique capabilities of this technique by presenting observations across myriad length scales, from the centimeter-scale down to the detection of sub-micrometer particles within these objects. Our initial results show the potential of this technique to help decipher spatial and textural variations in trace element chemistry between CAIs, chondrules, matrix, and other chondritic components. We also illustrate how these datasets can be applied to understanding both nebular and parent-body processes within meteorites.

Dyl, Kathryn A.; Cleverley, James S.; Bland, Phil A.; Ryan, Chris G.; Fisher, Louise A.; Hough, Robert M.

2014-06-01

267

Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor  

PubMed Central

Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in relation to short and long term periods of cold followed by a brief frost treatment were studied in order to disclose proteins responsible for the cold hardening process in distinct plant tissues. The mentioned changes have been monitored using two dimensional difference gel electrophoresis (2D-DIGE) with subsequent peptide-mapping protein identification. Regarding approximately 600–700 distinct protein spots detected on 2D gels, there has been found at least a two-fold change after exposure to low temperatures in about 10% of proteins in leaves and 13% of proteins in crowns. Protein and nitrogen metabolic processes have been influenced by low temperature to a similar extent in both tissues while catabolism, carbohydrate metabolism and proteins involved in stress response have been more affected in crowns than in leaves. The range of changes in protein abundance was generally higher in leaves and chloroplast proteins were frequently affected which suggests a priority to protect photosynthetic apparatus. Overall, our data proved existence of slightly different response strategies to low temperature stress in crowns and leaves, i.e., tissues with different biological role. Moreover, there have been found several proteins with large increase in accumulation, e.g., 33 kDa oxygen evolving protein of photosystem II in leaves and “enhanced disease susceptibility 1” in crowns; these proteins might have potential to indicate an enhanced level of frost tolerance in barley. PMID:23584021

Hlavá?ková, Iva; Vítámvás, Pavel; Šantr??ek, Ji?í; Kosová, Klára; Zelenková, Sylva; Prášil, Ilja Tom; Ovesná, Jaroslava; Hynek, Radovan; Kodí?ek, Milan

2013-01-01

268

Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency.  

PubMed

An efficient Agrobacterium-mediated durum wheat transformation system has been developed for the production of 121 independent transgenic lines. This improved system used Agrobacterium strain AGL1 containing the superbinary pGreen/pSoup vector system and durum wheat cv Stewart as the recipient plant. Acetosyringone at 400 microM was added to both the inoculation and cultivation medium, and picloram at 10 mg l(-1) and 2 mg l(-1) was used in the cultivation and induction medium, respectively. Compared with 200 microM in the inoculation and cultivation media, the increased acetosyringone concentration led to significantly higher GUS (beta-glucuronidase) transient expression and T-DNA delivery efficiency. However, no evident effects of acetosyringone concentration on regeneration frequency were observed. The higher acetosyringone concentration led to an improvement in average final transformation efficiency from 4.7% to 6.3%. Furthermore, the concentration of picloram in the co-cultivation medium had significant effects on callus induction and regeneration. Compared with 2 mg l(-1) picloram in the co-cultivation medium, increasing the concentration to 10 mg l(-1) picloram resulted in improved final transformation frequency from 2.8% to 6.3%, with the highest frequency of 12.3% reached in one particular experiment, although statistical analysis showed that this difference in final transformation efficiency had a low level of significance. Stable integration of foreign genes, their expression, and inheritance were confirmed by Southern blot analyses, GUS assay, and genetic analysis. Analysis of T(1) progeny showed that, of the 31 transgenic lines randomly selected, nearly one-third had a segregation ratio of 3:1, while the remainder had ratios typical of two or three independently segregating loci. PMID:20202997

He, Y; Jones, H D; Chen, S; Chen, X M; Wang, D W; Li, K X; Wang, D S; Xia, L Q

2010-06-01

269

A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata  

PubMed Central

Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays comprising 14?500 probes were used to compare, under three experimental conditions (in vitro, in the greenhouse, and in the vineyard), foliar material of infected symptomatic plants (S+R+), infected asymptomatic plants (S–R+), and healthy plants (S–R–). These plants were characterized by symptom notation after natural (vineyard) or experimental (in vitro and greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative real-time PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (Erysiphe necator, Plasmopara viticola, and Botrytis cinerea). (i) Five functional categories of genes, that is those involved in metabolism, defence reactions, interaction with the environment, transport, and transcription, were up-regulated in S+R+ plants compared with S–R– plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also up-regulated after infection by powdery mildew, downy mildew, and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and the polypeptide fraction secreted by Eutypa. PMID:20190040

Camps, Céline; Kappel, Christian; Lecomte, Pascal; Léon, Céline; Gomès, Eric; Coutos-Thévenot, Pierre; Delrot, Serge

2010-01-01

270

Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency  

PubMed Central

An efficient Agrobacterium-mediated durum wheat transformation system has been developed for the production of 121 independent transgenic lines. This improved system used Agrobacterium strain AGL1 containing the superbinary pGreen/pSoup vector system and durum wheat cv Stewart as the recipient plant. Acetosyringone at 400??M was added to both the inoculation and cultivation medium, and picloram at 10?mg l?1 and 2?mg l?1 was used in the cultivation and induction medium, respectively. Compared with 200??M in the inoculation and cultivation media, the increased acetosyringone concentration led to significantly higher GUS (?-glucuronidase) transient expression and T-DNA delivery efficiency. However, no evident effects of acetosyringone concentration on regeneration frequency were observed. The higher acetosyringone concentration led to an improvement in average final transformation efficiency from 4.7% to 6.3%. Furthermore, the concentration of picloram in the co-cultivation medium had significant effects on callus induction and regeneration. Compared with 2?mg l?1 picloram in the co-cultivation medium, increasing the concentration to 10?mg l?1 picloram resulted in improved final transformation frequency from 2.8% to 6.3%, with the highest frequency of 12.3% reached in one particular experiment, although statistical analysis showed that this difference in final transformation efficiency had a low level of significance. Stable integration of foreign genes, their expression, and inheritance were confirmed by Southern blot analyses, GUS assay, and genetic analysis. Analysis of T1 progeny showed that, of the 31 transgenic lines randomly selected, nearly one-third had a segregation ratio of 3:1, while the remainder had ratios typical of two or three independently segregating loci. PMID:20202997

He, Y.; Jones, H. D.; Chen, S.; Chen, X. M.; Wang, D. W.; Li, K. X.; Wang, D. S.; Xia, L. Q.

2010-01-01

271

Antioxidant Activity and Chemical Components as Potential Anticancer Agents in the Olive Leaf (Olea europaea L. cv Leccino.) Decoction.  

PubMed

Epidemiological studies have shown that a reduced risk of chronic diseases such as cancer and cardiovascular diseases is correlated with a regular consumption of fruits and vegetable, many of which are rich in polyphenols. The additive and synergistic effect of phytochemicals in fruits and vegetables may reduce chronic diseases related to oxidative stress in human body. Olea europaea L. leaf are rich in phenolic components, which have been proposed to play a role in cancer prevention. The purpose of this study was to identify the main components in the Olea europaea L. leaf (cv. Leccino) preserved during the decoction preparation, in order to delineate the antioxidant activities of the crude extracts and its isolated compounds by using different in vitro assays including DPPH radicalscavenging capacity, total antioxidant capacity (TAC), xanthine oxidase (XO) inhibitory effect and the ability to delay the linoleic acid peroxidation process (ALP). The aqueous decoction was partitioned obtaining four extracts and the n-butanol extract showed the highest antioxidant activity and the highest total phenolic content. Phytochemical investigation leads to the isolation of thirteen secondary metabolites including simple phenolics, flavonoids, secoiridoids whose structures were elucidated by spectroscopic data (1D and 2D NMR) and spectrometric techniques. A significant free radical scavenging effect against DPPH has been evidenced in fraxamoside (1) (EC50 62.6 µM) and taxifolin (5) (EC50 50.0 µM), isolated for the first time from the water decoction. The most active compound in the TAC evaluation, was the 3,4 dihydro-phenyl glycol (8) (0.90 caffeic acid equiv.) while taxifolin and fraxamoside resulted as the most efficient inhibitors of XO activity (IC50 2.7 and 5.2 µM, respectively). Secoxyloganin (4), oleuropein (2) and tyrosol (6) showed the highest ALP activity. This study adds to the growing body of data supporting the bioactivities of phytochemicals and their potential impact on human health. PMID:25102361

Marino, Simona De; Festa, Carmen; Zollo, Franco; Nini, Antonella; Antenucci, Lina; Raimo, Gennaro; Iorizzi, Maria

2014-01-01

272

A lectin with highly potent inhibitory activity toward breast cancer cells from edible tubers of Dioscorea opposita cv. nagaimo.  

PubMed

A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC(50) values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

Chan, Yau Sang; Ng, Tzi Bun

2013-01-01

273

Photosynthesis and growth responses of mustard (Brassica juncea L. cv Pusa Bold) plants to free air carbon dioxide enrichment (FACE).  

PubMed

Increased atmospheric [CO2] is likely to affect photosynthesis, plant growth, and yield potential of plants. Mustard (Brassica juncea L.) is an important oil seed crop that is widely grown in India. Therefore, the impact of elevated [CO2] (585 ?mol mol(-1)) on pigment and protein content, chlorophyll a fluorescence, photosynthetic electron transport reactions, CO2 assimilation, biomass production, and seed yield potential was measured in B. juncea cv Pusa Bold, grown inside free air carbon dioxide enrichment (FACE) rings installed on the campus of Jawaharlal Nehru University, New Delhi, India. Plants were grown for three consecutive winter seasons (2010-2013), in ambient (385 ?mol mol(-1)) or elevated [CO2], in field conditions. Elevated [CO2] had no significant effect on the minimal chlorophyll fluorescence (F 0), while the quantum efficiency of Photosystem II, measured as variable fluorescence (F v?=?F m-F 0) to maximum fluoresence (F m), increased by 3 %. Electron transport rate, photosystem I, photosystem II, and whole chain electron transport rates increased by 8 % in elevated [CO2]. However, the net photosynthesis rate increased by ?50 % in three growing seasons under elevated [CO2] condition. The stomatal conductance and transpiration rate decreased resulting in higher photosynthetic water use efficiency. The photosynthesizing surface, i.e., leaf area index substantially increased leading to higher biomass and seed yield under elevated [CO2] condition. Acclimatory downregulation of photosynthesis and plant productivity was not observed in three consecutive growing years suggesting that in the absence of nutrient limitation, B. juncea is highly responsive to elevated CO2 whose yield potential shall increase in changing climatic conditions. PMID:25471475

Ruhil, Kamal; Sheeba; Ahmad, Altaf; Iqbal, Muhammad; Tripathy, Baishnab C

2014-12-01

274

Xu Zhang's Homepage - CV  

E-print Network

Numerical Methods for Partial Differential Equations ... for Combined Hybrid Finite Element Methods and Application to Plate Bending Problems, Appl. Math. Comput. ... Fall 2014, MA 26600 (Ordinary Differential Equations), Purdue University.

275

Impact of innovative controlled atmosphere storage technologies and postharvest treatments on volatile compound production in cv. Pinova apples.  

PubMed

Organically grown apples cv. Pinova harvested at two different dates were stored at 1.3 degrees C for up to 4 months in air, up to 7 months in ULO (1.5 kPa of O(2) and 1.3 kPa of CO(2)) and in dynamic controlled atmosphere (DCA) conditions (0.4-0.6 kPa of O(2) and 0.6-0.8 kPa of CO(2)); the DCA storage method involved the use of a chlorophyll fluorescence monitoring system in order to detect low-O(2) stress in apples and to allow for the dynamic adaptation of storage atmosphere to O(2) levels that were lower than in ULO but still tolerated by fruits. A postharvest 1-MCP treatment (for 24 h at 1.3 degrees C) and a hot water treatment (for 180 s at 50 degrees C) were also tested on apples stored afterward in ULO and air, respectively. Volatile compounds isolated from the pulp of fruits were measured after 4 and 7 months, just upon removal from storage and after 11 days at 22 degrees C. Total amount of aroma compounds detected in apples stored in DCA was markedly higher (from 2- to 4-fold) than in fruits exposed to 1-MCP + ULO but, at most sampling times, significantly lower than in ULO fruits. Moderate differences in storage atmosphere composition between ULO and DCA significantly affected both total amount and profile of volatile esters. Analogous effects were observed on the alcohol precursors of the main esters. Exposure to 1-MCP inhibited biosynthesis of straight-chain esters more than that of branched-chain esters. The hot water treatment did not seem to produce marked changes in volatile composition after four months of air storage, except for a sharp accumulation of aldehydes during the shelf-life time. DCA storage technology, besides avoiding any chemical treatment, can preserve apple aroma compounds better than 1-MCP + ULO during long-term storage. PMID:19154103

Raffo, Antonio; Kelderer, Markus; Paoletti, Flavio; Zanella, Angelo

2009-02-11

276

Agrobacterium tumefaciens-mediated transformation of Indian mulberry, Morus indica cv. K2: a time-phased screening strategy.  

PubMed

An efficient and reproducible protocol for the production of transgenic plants was developed for Morus indica cv. K2 by Agrobacterium tumefaciens-mediated transformation. The hypocotyls, cotyledon, leaf and leaf callus explants precultured for 5 days on regeneration medium were co-cultivated with a bacterial suspension at 10(9) cells/ml for 3 days in the dark. Infectivity of A. tumefaciens strain LBA4404 was more than that of strains GV2260 and A281, and among the various plasmids tried, pBI121 and pBI101:Act1 transformed nearly 100% of the explants followed closely by p35SGUSINT. About 90-100% of the explants tested positive in the beta-glucuronidase (GUS) histochemical assay performed after 3 days of co-cultivation. This high level of transient expression, however, decreased to 20-25% after 15 days. Gus activity was most stable in the callus explants, which emerged as the explant of choice for transformation. The transformed explants were selected on 50-75 mg/l kanamycin for 1 month, and 25-50% of the explants developed adventitious buds. On the basis of kanamycin-resistant shoots produced from the total number of explants inoculated, the transformation efficiency was 44%. After 1 month, 40% of these shoots displayed high gus activity as assessed by the GUS fluorometric assay. On a selection-free root induction medium, 80% of the shoots developed roots and 90% of the potted plantlets acclimatized to the growth room conditions. The 3-month-old regenerates showed gus and nptII(neomycin phosphotransferase II) gene activity as assayed by the GUS fluorometric assay and nptII enzyme assay, followed by PCR polymerase chain reaction (54.5%) analysis after 6-months. Transgene integration into the nuclear genome of 1-year-old regenerates was confirmed in 10 of the 18 transformants tested by Southern analysis. The transformation efficiency as defined by the number of transgenic plants produced from the total number of explants co-cultivated was 6%. PMID:12789417

Bhatnagar, S; Khurana, P

2003-03-01

277

Efficacy of Pneumococcal Nontypable Haemophilus influenzae Protein D Conjugate Vaccine (PHiD-CV) in Young Latin American Children: A Double-Blind Randomized Controlled Trial  

PubMed Central

Background The relationship between pneumococcal conjugate vaccine–induced antibody responses and protection against community-acquired pneumonia (CAP) and acute otitis media (AOM) is unclear. This study assessed the impact of the ten-valent pneumococcal nontypable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) on these end points. The primary objective was to demonstrate vaccine efficacy (VE) in a per-protocol analysis against likely bacterial CAP (B-CAP: radiologically confirmed CAP with alveolar consolidation/pleural effusion on chest X-ray, or non-alveolar infiltrates and C-reactive protein ? 40 µg/ml); other protocol-specified outcomes were also assessed. Methods and Findings This phase III double-blind randomized controlled study was conducted between 28 June 2007 and 28 July 2011 in Argentine, Panamanian, and Colombian populations with good access to health care. Approximately 24,000 infants received PHiD-CV or hepatitis control vaccine (hepatitis B for primary vaccination, hepatitis A at booster) at 2, 4, 6, and 15–18 mo of age. Interim analysis of the primary end point was planned when 535 first B-CAP episodes, occurring ?2 wk after dose 3, were identified in the per-protocol cohort. After a mean follow-up of 23 mo (PHiD-CV, n?=?10,295; control, n?=?10,201), per-protocol VE was 22.0% (95% CI: 7.7, 34.2; one-sided p?=?0.002) against B-CAP (conclusive for primary objective) and 25.7% (95% CI: 8.4%, 39.6%) against World Health Organization–defined consolidated CAP. Intent-to-treat VE was 18.2% (95% CI: 5.5%, 29.1%) against B-CAP and 23.4% (95% CI: 8.8%, 35.7%) against consolidated CAP. End-of-study per-protocol analyses were performed after a mean follow-up of 28–30 mo for CAP and invasive pneumococcal disease (IPD) (PHiD-CV, n?=?10,211; control, n?=?10,140) and AOM (n?=?3,010 and 2,979, respectively). Per-protocol VE was 16.1% (95% CI: ?1.1%, 30.4%; one-sided p?=?0.032) against clinically confirmed AOM, 67.1% (95% CI: 17.0%, 86.9%) against vaccine serotype clinically confirmed AOM, 100% (95% CI: 74.3%, 100%) against vaccine serotype IPD, and 65.0% (95% CI: 11.1%, 86.2%) against any IPD. Results were consistent between intent-to-treat and per-protocol analyses. Serious adverse events were reported for 21.5% (95% CI: 20.7%, 22.2%) and 22.6% (95% CI: 21.9%, 23.4%) of PHiD-CV and control recipients, respectively. There were 19 deaths (n?=?11,798; 0.16%) in the PHiD-CV group and 26 deaths (n?=?11,799; 0.22%) in the control group. A significant study limitation was the lower than expected number of captured AOM cases. Conclusions Efficacy was demonstrated against a broad range of pneumococcal diseases commonly encountered in young children in clinical practice. Trial registration www.ClinicalTrials.gov NCT00466947 Please see later in the article for the Editors' Summary PMID:24892763

Tregnaghi, Miguel W.; Sáez-Llorens, Xavier; López, Pio; Abate, Hector; Smith, Enrique; Pósleman, Adriana; Calvo, Arlene; Wong, Digna; Cortes-Barbosa, Carlos; Ceballos, Ana; Tregnaghi, Marcelo; Sierra, Alexandra; Rodriguez, Mirna; Troitiño, Marisol; Carabajal, Carlos; Falaschi, Andrea; Leandro, Ana; Castrejón, Maria Mercedes; Lepetic, Alejandro; Lommel, Patricia; Hausdorff, William P.; Borys, Dorota; Guiñazú, Javier Ruiz; Ortega-Barría, Eduardo; Yarzábal, Juan P.; Schuerman, Lode

2014-01-01

278

Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish).  

PubMed

Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubens (Foc) is the most serious disease that attacks banana plants. Salicylic acid (SA) can play a key role in plant-microbe interactions. Our study is the first to examine the role of SA in conferring resistance to Foc TR4 in banana (Musa acuminata L. AAA group, cv. Cavendish), which is the greatest commercial importance cultivar in Musa. We used quantitative real-time reverse polymerase chain reaction (qRT-PCR) to analyze the expression profiles of 45 genes related to SA biosynthesis and downstream signaling pathways in a susceptible banana cultivar (cv. Cavendish) and a resistant banana cultivar (cv. Nongke No. 1) inoculated with Foc TR4. The expression of genes involved in SA biosynthesis and downstream signaling pathways was suppressed in a susceptible cultivar and activated in a resistant cultivar. The SA levels in each treatment arm were measured using high-performance liquid chromatography. SA levels were decreased in the susceptible cultivar and increased in the resistant cultivar. Finally, we examined the contribution of exogenous SA to Foc TR4 resistance in susceptible banana plants. The expression of genes involved in SA biosynthesis and signal transduction pathways as well as SA levels were significantly increased. The results suggest that one reason for banana susceptibility to Foc TR4 is that expression of genes involved in SA biosynthesis and SA levels are suppressed and that the induced resistance observed in banana against Foc TR4 might be a case of salicylic acid-dependent systemic acquired resistance. PMID:25277445

Wang, Zhuo; Jia, Caihong; Li, Jingyang; Huang, Suzhen; Xu, Biyu; Jin, Zhiqiang

2015-01-01

279

Understanding the atmospheric structure of T Tauri stars - II. UV spectroscopy of RY Tau, BP Tau, RU Lupi, GW Ori and CV Cha  

NASA Astrophysics Data System (ADS)

We report results from our study of International Ultraviolet Explorer (IUE) data of a group of T Tauri stars (TTS). Comparisons between UV-line fluxes in these stars and in the Sun indicate very high levels of activity in their atmospheres and comparatively higher electron densities. Spectroscopic diagnostic line ratios indicate densities over an order of magnitude higher than in the `quiet' Sun at `transition region' temperatures. At these densities, metastable levels can attain comparable populations to the ground level and ionization fractions can be altered as a result of the sensitivity of dielectronic recombination. In Brooks et al. we improved the treatment of these effects using the adas software package, the atomic models and data of which are based on collisional-radiative theory. Here we extend the analysis to a sample of five TTS: RY Tau, BP Tau, RU Lupi, GW Ori and CV Cha. Using these models and data we derive the emission measure (EM) distribution for each star in the sample. We find that the decrease in EM with increasing temperature appears to be sharper than that found in previous work. In comparison with the Sun, the results suggest that the UV emission is formed in a region with a steeper density or volume gradient. We find mismatches between the theoretical and observed fluxes which cannot be explained by density effects and thus must be a result of uncertainties in the atomic data, unreliabilities in the fluxes or the failure of physical assumptions in the method. We have made a series of tests and comparisons, including examination of opacity effects, and these clearly favour the latter explanation. They also lead us to suggest the presence of two separate components in the UV emission for each of the TTS, although the case of CV Cha is more ambiguous. This supports and extends the earlier work of Jordan & Kuin on RU Lupi. Interestingly, we find that the EM distribution for GW Ori has values at least 10 times larger than those of RY Tau, which appears to have approximately the same electron density. A similar difference is found between CV Cha and RU Lupi. Following geometrical arguments, we suggest that the UV emission in GW Ori and CV Cha is formed in a more extended region than in the other three stars.

Brooks, D. H.; Costa, V. M.; Lago, M. T. V. T.; Lanzafame, A. C.

2001-10-01

280

CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM1_Edition1-CV)  

NASA Technical Reports Server (NTRS)

The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-09-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

281

CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Terra-FM2_Edition1-CV)  

NASA Technical Reports Server (NTRS)

The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

282

CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Aqua-FM3_Edition1-CV)  

NASA Technical Reports Server (NTRS)

The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

283

CERES ERBE-like Instantaneous TOA Estimates (ES-8) in HDF (CER_ES8_Aqua-FM4_Edition1-CV)  

NASA Technical Reports Server (NTRS)

The ES-8 archival data product contains a 24-hour, single-satellite, instantaneous view of scanner fluxes at the top-of-atmosphere (TOA) reduced from spacecraft altitude unfiltered radiances using Earth Radiation Budget Experiment (ERBE) scanner Inversion algorithms and the ERBE shortwave (SW) and longwave (LW) Angular Distribution Models (ADMs). The ES-8 also includes the total (TOT), SW, LW, and window (WN) channel radiometric data; SW, LW, and WN unfiltered radiance values; and the ERBE scene identification for each measurement. These data are organized according to the CERES 3.3-second scan into 6.6-second records. As long as there is one valid scanner measurement within a record, the ES-8 record will be generated. The following CERES ES8 data sets are currently available: CER_ES8_TRMM-PFM_Edition1 CER_ES8_TRMM-PFM_Edition2 CER_ES8_TRMM-PFM_Transient-Ops2 CER_ES8_Terra-FM1_Edition1 CER_ES8_Terra-FM2_Edition1 CER_ES8_Terra-FM1_Edition2 CER_ES8_Terra-FM2_Edition2 CER_ES8_Aqua-FM3_Edition1 CER_ES8_Aqua-FM4_Edition1 CER_ES8_Aqua-FM3_Edition2 CER_ES8_Aqua-FM4_Edition2 CER_ES8_Aqua-FM3_Edition1-CV CER_ES8_Aqua-FM4_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV CER_ES8_Terra-FM1_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1997-12-27; Stop_Date=2005-03-29] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Temporal_Resolution=1 day; Temporal_Resolution_Range=Daily - < Weekly].

Wielicki, Bruce A. (Principal Investigator)

284

Gibberellins and N -(2-chloro-4-pyridyl)- N? -phenylurea improve retention force and reduce water core in pre-mature fruit of Japanese pear cv. Housui  

Microsoft Academic Search

A study of the effect of a gibberellin A3 + A4 mixture (GAs) on pre-harvest fruit drop of Japanese pear cv. Housui is reported. The GAs was applied alone or in combination\\u000a with N-(2-Chloro-4-pyridyl)-N?-phenylurea (CPPU) in a lanolin paste to the abscission zone at the spur-end of the pedicel. The results showed that the\\u000a GAs and CPPU combination treatment increased the pedicel–spur

Caixi Zhang; Kenji Tanabe; Ugyong Lee; Samsok Kang; Takashi Tokunaga

2009-01-01

285

In vitro hypoglycemic effects of different insoluble fiber-rich fractions prepared from the peel of Citrus sinensis L. cv. Liucheng.  

PubMed

Insoluble fiber-rich fractions (FRFs), including insoluble dietary fiber, alcohol-insoluble solid, and water-insoluble solid, were isolated from the peel of Citrus sinensis L. cv. Liucheng. We found that these three FRFs could effectively adsorb glucose, retard glucose diffusion, and inhibit the activity of alpha-amylase to different extents. These mechanisms might create a concerted benefit in decreasing the rate of glucose absorption and eventually lower the concentration of postprandial serum glucose. The potential hypoglycemic effects of these FRFs suggested that they could be incorporated as low-calorie bulk ingredients in high-fiber foods to reduce calorie level and control blood glucose level. PMID:14558787

Chau, Chi-Fai; Huang, Ya-Ling; Lee, Mao-Hsiang

2003-10-22

286

Induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of Vitis vinifera cv Monastrell.  

PubMed

Suspension-cultured cells of Vitis vinifera cv Monastrell were used to investigate the effects of methyljasmonate, ethylene and salicylic acid separately or in combination with cyclodextrins on both trans-resveratrol production and the induction of defense responses. The results showed that the addition of methyljasmonate or ethylene to suspension-cultured cells jointly treated with cyclodextrins and salicylic acid provoked a decrease of trans-resveratrol levels suggesting that salicylic acid has a negative and antagonistic effect with methyljasmonate or ethylene on trans-resveratrol production. Likewise, the exogenous application of these compounds induced the accumulation of pathogenesis-related proteins. Analysis of the extracellular proteome showed the presence of amino acid sequences homologous to an specific ?-1,3-glucanase, class III peroxidases and a ?-1,4-mannanase, which suggests that these signal molecules could play a role in mediating defense-related gene product expression in V. vinifera cv Monastrell. Apart from these inducible proteins, other proteins were found in both the control and elicited cell cultures of V. vinifera. These included class IV chitinase, polygalacturonase inhibitor protein and reticuline oxidase-like protein, suggesting that their expression is constitutive being involved in the modification of the cell wall architecture during cell culture growth and in the prevention of pathogen attack. PMID:23127362

Belchí-Navarro, Sarai; Almagro, Lorena; Sabater-Jara, Ana Belén; Fernández-Pérez, Francisco; Bru, Roque; Pedreño, Maria Angeles

2013-02-15

287

Multilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv ‘Fortune'  

PubMed Central

The genetic structure of 2n gametes and, particularly, the parental heterozygosity restitution at each locus depends on the meiotic process by which they originated, with first-division restitution and second-division restitution (SDR) being the two major mechanisms. The origin of 2n gametes in citrus is still controversial, although sexual polyploidisation is widely used for triploid seedless cultivar development. In this study, we report the analysis of 2n gametes of mandarin cv ‘Fortune' by genotyping 171 triploid hybrids with 35 simple sequence repeat markers. The microsatellite DNA allele counting-peak ratios method for allele-dosage evaluation proved highly efficient in segregating triploid progenies and allowed half-tetrad analysis (HTA) by inferring the 2n gamete allelic configuration. All 2n gametes arose from the female genitor. The observed maternal heterozygosity restitution varied between 10 and 82%, depending on the locus, thus SDR appears to be the mechanism underlying 2n gamete production in mandarin cv ‘Fortune'. A new method to locate the centromere, based on the best fit between observed heterozygosity restitution within a linkage group and theoretical functions under either partial or no chiasmata interference hypotheses was successfully applied to linkage group II. The maximum value of heterozygosity restitution and the pattern of restitution along this linkage group would suggest there is partial chiasma interference. The implications of such a restitution mechanism for citrus breeding are discussed. PMID:21587302

Cuenca, J; Froelicher, Y; Aleza, P; Juárez, J; Navarro, L; Ollitrault, P

2011-01-01

288

Direct online HPLC-CV-AFS method for traces of methylmercury without derivatisation: a matrix-independent method for urine, sediment and biological tissue samples.  

PubMed

Mercury (Hg) is a global pollutant which occurs in different species, with methylmercury (MeHg) being the critical compound due to its neurotoxicity and bioaccumulation through the food chain. Methods for trace speciation of MeHg are therefore needed for a vast range of sample matrices, such as biological tissues, fluids, soils or sediments. We have previously developed an ultra-trace speciation method for methylmercury in water, based on a preconcentration HPLC cold vapour atomic fluorescence spectrometry (HPLC-CV-AFS) method. The focus of this work is mercury speciation in a variety of sample matrices to assess the versatility of the method. Certified reference materials were used where possible, and samples were spiked where reference materials were not available, e.g. human urine. Solid samples were submitted for commonly used digestion or extraction processes to obtain a liquid sample for injection into the analytical system. For MeHg in sediment samples, an extraction procedure was adapted to accommodate MeHg separation from high amounts of Hg(2+) to avoid an overload of the column. The recovery for MeHg determination was found to be in the range of 88-104 % in fish reference materials (DOLT-2, DOLT-4, DORM-3), lobster (TORT-2), seaweed (IAEA-140/TM), sediments (ERM(®)-CC580) and spiked urine and has been proven to be robust, reliable, virtually matrix-independent and relatively cost-effective. Applications in the ultra-trace concentration range are possible using the preconcentration up to 200 mL, while for higher MeHg-containing samples, lower volumes can be applied. A comparison was carried out between species-specific isotope dilution gas chromatography inductively coupled plasma mass spectrometry (SSID-GC-ICP-MS) as the gold standard and HPLC-CV-AFS for biological tissues (liver, kidney and muscle of pilot whales), showing a slope of 1.008 and R (2)?=?0.97, which indicates that the HPLC-CV-AFS method achieves well-correlated results for MeHg in biological tissues. PMID:25318461

Brombach, Christoph-Cornelius; Gajdosechova, Zuzana; Chen, Bin; Brownlow, Andrew; Corns, Warren T; Feldmann, Jörg; Krupp, Eva M

2015-01-01

289

Noble Gas Analysis of the Julesburg L3.6, Tulia H4, Y-86789 Thermally Metamorphosed CM, and Allende CV3 Chondrites by Crushing Method  

NASA Astrophysics Data System (ADS)

We analyzed noble gases of the Julesburg L3.6, Tulia H4, Y-86789 thermally metamorphosed CM, and Allende CV3 chondrites by the stepped crushing method. All samples have released light noble gases with meteoritic isotopic compositions and heavy noble gases which are contaminated with air to various extents. Total amounts of crash-released noble gases of the four meteorites are estimated to be less than 1% of bulk. Comparing with the results of crushing experiments on the Happy Canyon E6/7 chondrite, the above four meteorites released much smaller fractions of noble gases, suggesting that the four meteorites contain minor amounts of noble gases in places such as microbubbles.

Fukuda, D.; Nakamura, T.; Takaoka, N.; Nagao, K.

1996-03-01

290

Determination of replacement of some inorganic elements in pulvinus of bean (Phaseolus vulgaris cv. Gina 2004) at chilling temperature by the WDXRF spectroscopic technique  

NASA Astrophysics Data System (ADS)

In this study, bean seedlings (Phaseolus vulgaris cv. Gina 2004) were exposed to chilling temperatures until leaves are wrinkled (9 day), that is, showed nyctinastic movement. Pulvinus were subsequently were cut from the leaves. Concentrations of inorganic elements (P, S, Cl, K, Ca, Cu) in the pulvinus were measured by wavelength-dispersive X-ray fluorescence (WDXRF) spectrometry. Results indicated that concentration change (%) was not significant for Ca (0.82) but it was significant for K, P, Cl, S, and especially Cu concentrations (5.4%, 12.8%, 40.2%, 43.7%, 365%, respectively) in pulvinus of plants exposed to chilling temperature compared with control group. We hypothesize here the presence of association between nyctinasti movement brought about by pulvinus at chilling temperature in bean and changes of K, P, Cl, S and especially Cu concentrations measured by WDXRF analysis method.

Dumlupinar, Rahmi; Demir, Faruk; Budak, Gokhan; Karabulut, Abdulhalik; Kadi, Nuray; Karakurt, Halil; Erdal, Serkan

2007-01-01

291

CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Terra-FM1_Edition1-CV)  

NASA Technical Reports Server (NTRS)

The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2006-09-30] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal_Resolution_Range=250 km - < 500 km or approximately 2.5 degrees - < 5.0 degrees; Temporal_Resolution=1 month; Temporal_Resolution_Range=Monthly - < Annual].

Wielicki, Bruce A. (Principal Investigator)

292

CERES ERBE-like Monthly Geographical Averages (ES-4) in HDF (CER_ES4_Terra-FM2_Edition1-CV)  

NASA Technical Reports Server (NTRS)

The ERBE-like Monthly Geographical Averages (ES-4) product contains a month of space and time averaged Clouds and the Earth's Radiant Energy System (CERES) data for a single scanner instrument. The ES-4 is also produced for combinations of scanner instruments. For each observed 2.5-degree spatial region, the daily average, the hourly average over the month, and the overall monthly average of shortwave and longwave fluxes at the Top-of-the-Atmosphere (TOA) from the CERES ES-9 product are spatially nested up from 2.5-degree regions to 5- and 10-degree regions, to 2.5-, 5-, and 10-degree zonal averages, and to global monthly averages. For each nested area, the albedo and net flux are given. For each region, the daily average flux is estimated from an algorithm that uses the available hourly data, scene identification data, and diurnal models. This algorithm is 'like' the algorithm used for the Earth Radiation Budget Experiment (ERBE). The following CERES ES4 data sets are currently available: CER_ES4_FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition1 CER_ES4_PFM+FM1+FM2_Edition2 CER_ES4_PFM+FM1_Edition1 CER_ES4_PFM+FM2_Edition1 CER_ES4_TRMM-PFM_Edition1 CER_ES4_TRMM-PFM_Edition2 CER_ES4_Terra-FM1_Edition1 CER_ES4_Terra-FM2_Edition1 CER_ES4_FM1+FM2_Edition2 CER_ES4_Terra-FM1_Edition2 CER_ES4_Terra-FM2_Edition2 CER_ES4_Aqua-FM3_Edition1 CER_ES4_Aqua-FM4_Edition1 CER_ES4_FM1+FM2+FM3+FM4_Edition1 CER_ES4_Aqua-FM3_Edition2 CER_ES4_Aqua-FM4_Edition2 CER_ES4_FM1+FM3_Edition2 CER_ES4_FM1+FM4_Edition2 CER_ES4_PFM+FM1_Edition2 CER_ES4_PFM+FM2_Edition2 CER_ES4_Aqua-FM3_Edition1-CV CER_ES4_Aqua-FM4_Edition1-CV CER_ES4_Terra-FM1_Edition1-CV CER_ES4_Terra-FM2_Edition1-CV. [Location=GLOBAL] [Temporal_Coverage: Start_Date=1998-01-01; Stop_Date=2006-10-31] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180] [Data_Resolution: Latitude_Resolution=2.5 degree; Longitude_Resolution=2.5 degree; Horizontal_Resolution_Range=250 km - < 500 km or approximately 2.5 degrees - < 5.0 degrees; Temporal_Resolution=1 month; Temporal_Resolution_Range=Monthly - < Annual].

Wielicki, Bruce A. (Principal Investigator)

293

Introducing constricted variational density functional theory in its relaxed self-consistent formulation (RSCF-CV-DFT) as an alternative to adiabatic time dependent density functional theory for studies of charge transfer transitions  

SciTech Connect

We have applied the relaxed and self-consistent extension of constricted variational density functional theory (RSCF-CV-DFT) for the calculation of the lowest charge transfer transitions in the molecular complex X-TCNE between X = benzene and TCNE = tetracyanoethylene. Use was made of functionals with a fixed fraction (?) of Hartree-Fock exchange ranging from ? = 0 to ? = 0.5 as well as functionals with a long range correction (LC) that introduces Hartree-Fock exchange for longer inter-electronic distances. A detailed comparison and analysis is given for each functional between the performance of RSCF-CV-DFT and adiabatic time-dependent density functional theory (TDDFT) within the Tamm-Dancoff approximation. It is shown that in this particular case, all functionals afford the same reasonable agreement with experiment for RSCF-CV-DFT whereas only the LC-functionals afford a fair agreement with experiment using TDDFT. We have in addition calculated the CT transition energy for X-TCNE with X = toluene, o-xylene, and naphthalene employing the same functionals as for X = benzene. It is shown that the calculated charge transfer excitation energies are in as good agreement with experiment as those obtained from highly optimized LC-functionals using adiabatic TDDFT. We finally discuss the relation between the optimization of length separation parameters and orbital relaxation in the RSCF-CV-DFT scheme.

Krykunov, Mykhaylo; Seth, Mike; Ziegler, Tom [Department of Chemistry, University of Calgary, University Drive 2500, Calgary, Alberta T2N 1N4 (Canada)] [Department of Chemistry, University of Calgary, University Drive 2500, Calgary, Alberta T2N 1N4 (Canada)

2014-05-14

294

Smoking cessation and the risk of cardiovascular disease outcomes predicted from established risk scores: Results of the Cardiovascular Risk Assessment among Smokers in Primary Care in Europe (CV-ASPIRE) Study  

PubMed Central

Background Smoking is a major risk factor for cardiovascular disease (CVD). This multicenter, cross-sectional survey was designed to estimate the cardiovascular (CV) risk attributable to smoking using risk assessment tools, to better understand patient behaviors and characteristics related to smoking, and characterize physician practice patterns. Methods 1,439 smokers were recruited from Europe during 2011. Smokers were ?40 years old, smoked > 10 cigarettes/day and had recent measurements on blood pressure and lipids. CV risk was calculated using the SCORE system, Framingham risk equations, and Progetto CUORE model. The CV risk attributable to smoking was evaluated using a simulated control (hypothetical non-smoker) with identical characteristics as the enrolled smoker. Risks assessed included CV mortality, coronary heart disease (CHD), CVD and hard CHD. Demographics, comorbidities, primary reasons for consultation, behavior towards previous attempts to quit, and interest in smoking cessation was assessed. Dependence on nicotine was evaluated using the Fagerström Test for Nicotine Dependence. GP practice patterns were assessed through a questionnaire. Results The prediction models consistently demonstrated a high CV risk attributable to smoking. For instance, the SCORE model demonstrated that this study population of smokers have a 100% increased probability of death due to cardiovascular disease in the next 10-years compared to non-smokers. A considerable amount of patients would like to hear from their GP about the different alternatives available to support their quitting attempt. Conclusions The findings of this study reinforce the importance of smoking as a significant predictor of long-term cardiovascular events. One of the best gains in health could be obtained by tackling the most important modifiable risk factors; these results suggest smoking is among the most important. PMID:23597191

2013-01-01

295

Developmental patterns of emission of scent compounds and related gene expression in roses of the cultivar Rosa x hybrida cv. 'Yves Piaget'.  

PubMed

2-Phenylethanol (2PE) and 3,5-dimethoxytoluene (DMT) are characteristic scent compounds in specific roses such as Rosa x hybrida cv. 'Yves Piaget'. We analyzed the endogenous concentrations and emission of 2PE and DMT during the unfurling process in different floral organs, as well as changes in transcript levels of the two key genes, PAR and OOMT2. The emission of both 2PE and DMT increased during floral development to reach peaks at the fully unfurled stage. The relative transcripts of PAR and OOMT2 also increased during floral development. Whereas the maximum for OOMT2 was found at the fully unfurled stage (stage 4), similar expression levels of PAR were detected at stage 4 and the senescence stage (stage 6). The results demonstrate a positive correlation between the expression levels of PAR and OOMT2 and the emission of 2PE and DMT. In addition, endogenous volatiles and relative transcripts showed tissue- and development-specific patterns. PMID:25576838

Chen, Xiaomin; Baldermann, Susanne; Cao, Shuyan; Lu, Yao; Liu, Caixia; Hirata, Hiroshi; Watanabe, Naoharu

2015-02-01

296

Characterization and sugar-binding properties of arcelin-1, an insecticidal lectin-like protein isolated from kidney bean (Phaseolus vulgaris L. cv. RAZ-2) seeds.  

PubMed Central

Arcelin-1 is a lectin-like protein found in the seeds of wild varieties of the kidney bean (Phaseolus vulgaris). This protein displays insecticidal properties, but the mechanism of action is as yet unknown. In the present study we investigated the biochemical and biophysical properties of arcelin-1 from Phaseolus vulgaris cv. RAZ-2. Native arcelin-1 is a dimeric glycoprotein of 60 kDa, built from the non-covalent association of two identical monomers. This dimer resists dissociation by chaotropic agents and is highly resistant to proteolytic enzymes. Each subunit contains 10% (w/w) neutral sugars which belong to the high-mannose and complex-type glycans attached to three glycosylation sites. No interaction of the protein with simple sugars could be detected, but arcelin-1 displays an intrinsic specificity in binding complex glycans. Arcelin-1 therefore differs from the closely related phytohaemagglutinin lectins and alpha-amylase inhibitor in several respects: oligomerization states, sugar-binding affinities and the type and number of glycan chains. These features may be related to the toxicity of arcelin-1. PMID:9445382

Fabre, C; Causse, H; Mourey, L; Koninkx, J; Rivière, M; Hendriks, H; Puzo, G; Samama, J P; Rougé, P

1998-01-01

297

Effects of modified atmosphere packing and honey dip treatments on quality maintenance of minimally processed grape cv. Razaki (V. vinifera L.) during cold storage.  

PubMed

Increasing pressure in food conservation sector to replace chemical applications has urged researchers to focus on studying new strategies of extending the postharvest life of produces. In such efforts, numerous materials have been tested for their effectiveness as well as suitability in organic consumption. In this study, effects of modified atmosphere packing (MAP) and honey solution dip on maintenance of quality of minimally processed table grape cv. Razaki were investigated. During the storage at 0 °C with relative humidity of 90%, MAP, honey dip, and their combined applications significantly retarded the weight loss of berries that retained about 2 mm of cap stem. Soluble solid contents of all berries slightly increased, while their acid amounts decreased, resulting in consecutive rises of maturity index. With respect to the sensory score, calculated as mean of ten panelists, honey treatment alone was ranked the highest while control berries had significantly lower value. Overall, MAP, honey solution dip or their combination significantly maintained the general quality of minimally processed grape by delaying quality loss and berry decay. Therefore, honey solution dip yielded promising results to use as an edible organic coating barrier to moisture and resist to water vapor diffusion during the cold storage, offering a good adherence to berry surface. PMID:23572752

Sab?r, Ali; Sab?r, Ferhan K; Kara, Zeki

2011-06-01

298

Relationships between harvest time and wine composition in Vitis vinifera L. cv. Cabernet Sauvignon 2. Wine sensory properties and consumer preference.  

PubMed

A series of five Vitis vinifera L. cv Cabernet Sauvignon wines were produced from sequentially-harvested grape parcels, with alcohol concentrations between 12% v/v and 15.5% v/v. A multidisciplinary approach, combining sensory analysis, consumer testing and detailed chemical analysis was used to better define the relationship between grape maturity, wine composition and sensory quality. The sensory attribute ratings for dark fruit, hotness and viscosity increased in wines produced from riper grapes, while the ratings for the attributes red fruit and fresh green decreased. Consumer testing of the wines revealed that the lowest-alcohol wines (12% v/v) were the least preferred and wines with ethanol concentration between 13% v/v and 15.5% v/v were equally liked by consumers. Partial least squares regression identified that many sensory attributes were strongly associated with the compositional data, providing evidence of wine chemical components which are important to wine sensory properties and consumer preferences, and which change as the grapes used for winemaking ripen. PMID:24518320

Bindon, Keren; Holt, Helen; Williamson, Patricia O; Varela, Cristian; Herderich, Markus; Francis, I Leigh

2014-07-01

299

Changes in the phenolic composition of virgin olive oil from young trees (Olea europaea L. cv. Arbequina) grown under linear irrigation strategies.  

PubMed

This study reports the HPLC profiles of phenolic compounds of virgin olive oils obtained from young olive trees (Olea europaea L. cv. Arbequina) and how the application of a linear irrigation strategy affected these. Hydroxytyrosol, tyrosol, vanillic acid, vanillin, 4-(acetoxyethyl)-1,2-dihydroxybenzene, p-coumaric acid, the dialdehydic form of elenolic acid linked to hydroxytyrosol and to tyrosol, lignans, and the oleuropein aglycon were found in all the oils. Hydroxytyrosol, tyrosol, vanillic acid, and p-coumaric acid contents in the oils were unaffected by linear irrigation. The concentration of lignans was lower in the oils from the least irrigated treatment and the concentration of vanillin increased as the amount of irrigation water applied to olive trees increased. However, 4-(acetoxyethyl)-1,2-dihydroxybenzene, the dialdehydic form of elenolic acid linked to hydroxytyrosol and to tyrosol, and the oleuropein aglycon, all of them hydroxyphenyl derivatives, decreased as the level of irrigation water increased. The latter three compounds represented the most considerable part of the phenolic fraction of the oils and they were shown to be correlated to the oxidative stability, the bitter index (K(225)), and the bitter, pungent, and sweet sensory attributes. Linear irrigation strategy changed the profile of the oil phenolic compounds and, therefore, changed both the organoleptic properties and the antioxidant capacity of the product. PMID:11714351

Tovar, M J; Motilva, M J; Romero, M P

2001-11-01

300

Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK  

PubMed Central

Components from Dendranthema × grandiflorum cv. “Mottenohoka” that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth. PMID:23554829

Kimura, Hirokazu; Tsukagoshi, Hiroyuki; Kozawa, Kunihisa; Koketsu, Mamoru; Ninomiya, Masayuki; Furukawa, Shoei

2013-01-01

301

Melatonin levels, determined by LC-ESI-MS/MS, fluctuate during the day/night cycle in Vitis vinifera cv Malbec: evidence of its antioxidant role in fruits.  

PubMed

The identification of melatonin in plants has inspired new investigations to understand its biological function and which endogenous and external factors control its levels in these organisms. Owing to the therapeutical and nutraceutical properties of melatonin, it should be important to develop reliable analytical methods for its quantification in vegetal matrices containing this indoleamine, such as grape and wine. The main objectives of the present study were to test whether melatonin levels fluctuate during the day in berry skins of Vitis vinifera L. cv Malbec, thereby possibly relating its abundance to its putative antioxidant function, to determine whether daylight reaching clusters negatively controls melatonin levels, and to evaluate whether total polyphenols and anthocyanins also change through a 24-hr period. Grapes were harvested throughout the day/night to determine the moment when high levels of these components are present in grapes. The presence of melatonin in grapes was evaluated by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry. It is shown for the first time that melatonin levels fluctuate during the day/night cycle in plants grown under field conditions in a fruit organ of the species Vitis vinifera. We also determined that the diurnal decay of melatonin in berry skins is induced by sunlight, because covered bunches retained higher melatonin levels than exposed ones, thus explaining at least part of the basis of its daily fluctuation. Evidence of melatonin's antioxidant role in grapes is also suggested by monitoring malondialdehyde levels during the day. PMID:21605162

Boccalandro, Hernán E; González, Carina V; Wunderlin, Daniel A; Silva, María F

2011-09-01

302

Field testing, gene flow assessment and pre-commercial studies on transgenic Solanum tuberosum spp. tuberosum (cv. Spunta) selected for PVY resistance in Argentina.  

PubMed

Solanum tuberosum ssp. tuberosum (cv. Spunta) was transformed with a chimeric transgene containing the Potato virus Y (PVY) coat protein (CP) sequence. Screening for PVY resistance under greenhouse conditions yielded over 100 independent candidate lines. Successive field testing of selected lines allowed the identification of two genetically stable PVY-resistant lines, SY230 and SY233, which were further evaluated in field trials at different potato-producing regions in Argentina. In total, more than 2,000 individuals from each line were tested along a 6-year period. While no or negligible PVY infection was observed in the transgenic lines, infection rates of control plants were consistently high and reached levels of up to 70-80%. Parallel field studies were performed in virus-free environments to assess the agronomical performance of the selected lines. Tubers collected from these assays exhibited agronomical traits and biochemical compositions indistinguishable from those of the non-transformed Spunta cultivar. In addition, an interspecific out-crossing trial to determine the magnitude of possible natural gene flow between transgenic line SY233 and its wild relative Solanum chacoense was performed. This trial yielded negative results, suggesting an extremely low probability for such an event to occur. PMID:22200984

Bravo-Almonacid, Fernando; Rudoy, Valeria; Welin, Bjorn; Segretin, María Eugenia; Bedogni, María Cecilia; Stolowicz, Fabiana; Criscuolo, Marcelo; Foti, Marcelo; Gomez, Maximiliano; López, Mariana; Serino, Germán; Cabral, Silvia; Dos Santos, Cristina; Huarte, Marcelo; Mentaberry, Alejandro

2012-10-01

303

Interface and plasma damage analysis of PEALD TaCN deposited on HfO2 for advanced CMOS studied by angle resolved XPS and C-V  

NASA Astrophysics Data System (ADS)

Plasma enhanced atomic layer deposition (PEALD) TaCN deposited on HfO2 was studied by X-ray photoelectron spectroscopy (XPS) to understand the reactions taking place at the interface and connect them with C-V electrical characteristics of MOS devices. Moreover, angular resolved XPS (AR-XPS) was used for composition depth profiling of TaCN/HfO2/SiO2/Si stacks. Clear oxidation of the metal electrode through Tasbnd O bonding formation and migration of N in the dielectric with Hfsbnd N are shown. These modifications of chemical bonding give an insight on the electrical results. Low equivalent oxide thicknesses (EOT), as low as 0.89 nm and current leakage improvement by more than 5 decades, are observed for deposition with low plasma power and can be related to HfN content in HfO2 layer. The increase of plasma power used for TaCN deposition results in densification of the layer and promotes the creation of TaC in TaCN material. However H2 plasma has an impact on HfO2 with a reduction and scattering of the measured current leak gain. TaCN/HfO2 interface is also impacted with further creation of TaOx, leading to an increase of EOT when plasma power is increased. Based on these findings, reaction mechanisms with the corresponding Gibbs free energy are proposed.

Piallat, Fabien; Beugin, Virginie; Gassilloud, Remy; Dussault, Laurent; Pelissier, Bernard; Leroux, Charles; Caubet, Pierre; Vallée, Christophe

2014-06-01

304

The photosynthetic and stomatal response of Medicago sativa cv. saranac to free-air CO{sub 2} enrichment (F.A.C.E.) and nitrogen  

SciTech Connect

Plots of Medicago sativa cv. saranac were grown in the field at ambient (355 {mu}mol CO{sub 2} mol{sup -1} air) or elevated (600{mu}mol CO{sub 2} mol{sup -1} air) CO{sub 2} concentrations. High (200kg yr{sup -1}) or low (20kg yr{sup -1}) nitrogen levels were applied to two isogeneic lines, one able and one unable to use nitrogen fixing bacteria. Plants were in the second year of field growth. Exposure to elevated CO{sub 2} was via a Free-Air CO{sub 2} Enrichment System (FACE). Elevated CO{sub 2} increased diurnal assimilation by between 12% and 92%. Analysis of A/C{sub i} responses showed that effective nitrogen fertilisation was more important to rubisCO and RuBP activity than elevated CO{sub 2}. No acclimation was consistently observed. Leaves lower down the canopy were found to have lower Vc{sub max} and J{sub max} values, though age may be the cause of the latter effect. FACE conditions have only a small effect on these responses. There was some evidence found for the down-regulation of photosynthesis in the late afternoon. The FACE conditions had no affect on stomatal density but did increase epidermal cell density.

Bridson, N.P.

1996-08-01

305

Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway.  

PubMed

The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7 ?g/g dry weight (DW) and a maximum of 31.3 ?g/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3 ?g/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag. PMID:24530945

Ritala, Anneli; Dong, Lemeng; Imseng, Nicole; Seppänen-Laakso, Tuulikki; Vasilev, Nikolay; van der Krol, Sander; Rischer, Heiko; Maaheimo, Hannu; Virkki, Arho; Brändli, Johanna; Schillberg, Stefan; Eibl, Regine; Bouwmeester, Harro; Oksman-Caldentey, Kirsi-Marja

2014-04-20

306

Promoting Effect of Foliage Sprayed Zinc Sulfate on Accumulation of Sugar and Phenolics in Berries of Vitis vinifera cv. Merlot Growing on Zinc Deficient Soil.  

PubMed

The effect of foliage sprayed zinc sulfate on berry development of Vitis vinifera cv. Merlot growing on arid zone Zn-deficient soils was investigated over two consecutive seasons, 2013 and 2014. Initial zinc concentration in soil and vines, photosynthesis at three berry developmental stages, berry weight, content of total soluble solids, titratable acidity, phenolics and expression of phenolics biosynthetic pathway genes throughout the stages were measured. Foliage sprayed zinc sulfate showed promoting effects on photosynthesis and berry development of vines and the promotion mainly occurred from veraison to maturation. Zn treatments enhanced the accumulation of total soluble solids, total phenols, flavonoids, flavanols, tannins and anthocyanins in berry skin, decreasing the concentration of titratable acidity. Furthermore, foliage sprayed zinc sulfate could significantly influence the expression of phenolics biosynthetic pathway genes throughout berry development, and the results of expression analysis supported the promotion of Zn treatments on phenolics accumulation. This research is the first comprehensive and detailed study about the effect of foliage sprayed Zn fertilizer on grape berry development, phenolics accumulation and gene expression in berry skin, providing a basis for improving the quality of grape and wine in Zn-deficient areas. PMID:25648596

Song, Chang-Zheng; Liu, Mei-Ying; Meng, Jiang-Fei; Chi, Ming; Xi, Zhu-Mei; Zhang, Zhen-Wen

2015-01-01

307

Heat shock transcription factors expression during fruit development and under hot air stress in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit.  

PubMed

Heat shock transcription factors (Hsfs) play a role in plant responses to stress. Citrus is an economically important fruit whose genome has been fully sequenced. So far, no detailed characterization of the Hsf gene family is available for citrus. A genome-wide analysis was carried out in Citrus clementina to identify Hsf genes, named CcHsfs. Eighteen CcHsfs were identified and classified into three main clades (clades A, B and C) according to the structural characteristics and the phylogenetic comparison with Arabidopsis and tomato. MEME motif analysis highlighted the conserved DBD and HR-A/B domains, which were similar to Hsf protein structures in other species. Gene expression analysis in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit identified 14 Hsf genes, named CrHsf, as important candidates for a role in fruit development and ripening, and showed seven genes to be expressed in response to hot air stress. CrHsfB2a and CrHsfB5 were considered to be important regulators of citrate content and showed variation in both developmentally-related and hot air-triggered citrate degradation processes. In summary, the data obtained from this investigation provides the basis for further study to dissect Hsf function during fruit development as well as in response to heat stress and also emphasizes the potential importance of CrHsfs in regulation of citrate metabolism in citrus fruit. PMID:25596345

Lin, Qiong; Jiang, Qing; Lin, Juanying; Wang, Dengliang; Li, Shaojia; Liu, Chunrong; Sun, Chongde; Chen, Kunsong

2015-04-01

308

Complete nucleotide sequences of three dsRNA segments from Raphanus sativus-root cv. Yidianhong [corrected] with leaf yellow edge symptoms.  

PubMed

The two minor dsRNA bands, previously detected in symptomatic leaves of Raphanus sativus-root cv. Yidianhong [corrected] were subjected to further analysis. cDNA cloning and sequencing revealed that the smaller of the two dsRNA bands is actually a doublet consisting of two co-migrating dsRNA segments and the resulting three segments were designated as RasR 3, RasR 4, and RasR 5. RasR 3 was 1717 bp in length and potentially encoded a protein of about 55.3 kDa, containing all of the six conserved motifs shared by the RNA dependent RNA polymerases of members of the family Partitiviridae. RasR 4 and RasR 5, which co-migrated in the 5% polyacrylamide gel, were 1521 and 1485 bp in length and each encoded a putative protein of unknown function. Their molecular masses, as calculated from the deduced amino acid, were 38.2 and 38.8 kDa, respectively. The 5' UTRs of all three segments shared regions of high sequence similarities, but were distinct from those of the RasR 1 and RasR 2. Taken together, these results along with those described in the previous report, suggest that more than one partitivirus was co-infecting radish leaves. PMID:16639633

Chen, L; Chen, J S; Zhang, H; Chen, S N

2006-10-01

309

Complete nucleotide sequences and genome characterization of double-stranded RNA 1 and RNA 2 in the Raphanus sativus-root cv. Yidianhong [corrected].  

PubMed

Four distinct double-stranded (ds) RNA bands were extracted from leaves of Raphanus sativus-root cv. Yidianhong [corrected] with yellowing at the leaf edge in China. Purified viral particles of 28-30 nm in diameter contained dsRNA segments with the same number and mobility as these extracted directly from radish leaves. The two major dsRNA segments, namely RasR 1 and RasR 2, were 1866 and 1791 bp in length, respectively. Computer analysis predicted that they both contained a single open reading frame (ORF) on their plus-stranded RNA, putatively encoding a RNA dependent RNA polymerase and a capsid protein similar to that encoded by members of the family Partitiviridae. In addition, both RasR 1 and RasR 2 were highly conserved at the 5' untranslated regions (UTR) and had an adenosine-uracil rich stretch at the 3' UTR, with an identical terminal motif (5'-AAAAUAAAACC-3'). Taken together, these results suggest that the two major dsRNA segments constitute the genome of a partitivirus infecting radish. PMID:16369860

Chen, L; Chen, J S; Liu, L; Yu, X; Yu, S; Fu, T Z; Liu, W H

2006-05-01

310

Germacrene C synthase from Lycopersicon esculentum cv. VFNT Cherry tomato: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene?cyclase  

PubMed Central

Germacrene C was found by GC-MS and NMR analysis to be the most abundant sesquiterpene in the leaf oil of Lycopersicon esculentum cv. VFNT Cherry, with lesser amounts of germacrene A, guaia-6,9-diene, germacrene B, ?-caryophyllene, ?-humulene, and germacrene D. Soluble enzyme preparations from leaves catalyzed the divalent metal ion-dependent cyclization of [1-3H]farnesyl diphosphate to these same sesquiterpene olefins, as determined by radio-GC. To obtain a germacrene synthase cDNA, a set of degenerate primers was constructed based on conserved amino acid sequences of related terpenoid cyclases. With cDNA prepared from leaf epidermis-enriched mRNA, these primers amplified a 767-bp fragment that was used as a hybridization probe to screen the cDNA library. Thirty-one clones were evaluated for functional expression of terpenoid cyclase activity in Escherichia coli by using labeled geranyl, farnesyl, and geranylgeranyl diphosphates as substrates. Nine cDNA isolates expressed sesquiterpene synthase activity, and GC-MS analysis of the products identified germacrene C with smaller amounts of germacrene A, B, and D. None of the expressed proteins was active with geranylgeranyl diphosphate; however, one truncated protein converted geranyl diphosphate to the monoterpene limonene. The cDNA inserts specify a deduced polypeptide of 548 amino acids (Mr = 64,114), and sequence comparison with other plant sesquiterpene cyclases indicates that germacrene C synthase most closely resembles cotton ?-cadinene synthase (50% identity). PMID:9482865

Colby, Sheila M.; Crock, John; Dowdle-Rizzo, Barbara; Lemaux, Peggy G.; Croteau, Rodney

1998-01-01

311

Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901  

PubMed Central

Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit. PMID:23615464

Zhu, Xiaoyan; Luo, Fenglei; Zheng, Yixiong; Zhang, Jiukai; Huang, Jianzhen; Sun, Chongde; Li, Xian; Chen, Kunsong

2013-01-01

312

Effect of a low dose of aluminum on mitotic and meiotic activity, 4C DNA content, and pollen sterility in rice, Oryza sativa L. cv. Lalat.  

PubMed

Aluminum toxicity in acidic soils poses a major threat to plant growth and development. The effects of a low dose of aluminum (50 microM, AlCl3) on various cytological parameters, including mitotic and meiotic chromosomal divisions, in situ nuclear DNA content, interphase nuclear volume (INV), and pollen fertility were compared in untreated (controls) and treated rice plants (Oryza sativa cv. Lalat). The results showed varied chromosomal abnormalities, including chromosome stickiness, laggards, sticky bridge, occurrence of micronuclei, as well as binucleate and multinucleated cells, as a result of aluminum treatment. Aluminum toxicity also inhibited to a small extent the growth of the rice cultivar Lalat. The mitotic and meiotic indexes, even after a prolonged period of recovery, were significantly low. The chromosomal anomalies in the meiotic cells persisted, and plants exhibited a high percentage of pollen sterility (approximately 64%). The nuclear DNA content declined markedly from 11.85 pg in the control to 6.30 pg in the treated plants. The INV also varied significantly between the untreated (controls) and the treated plants. The occurrences of different types of chromosomal aberrations, reduction in the amount of nuclear DNA, and persistence of the phytotoxic effects at the post-treatment stage suggest carcinogenic effects of aluminum on rice plants. The presence of aluminum in acidic soils might thus be extremely hazardous and might cause permanent cytotoxic disorder in rice plants. PMID:15261725

Mohanty, Suprava; Das, Anath Bandhu; Das, Premananda; Mohanty, Prasanna

2004-09-01

313

Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice (Oryza sativa L. sub sp. indica cv. ADT 43).  

PubMed

A simple and efficient protocol for the Agrobacterium-mediated transformation of an agronomically useful abiotic sensitive popular indica rice cv. ADT 43 has been developed. Initiation of calli were best achieved from the leaf bases of 4 days old rice seedlings on LS medium supplemented with 2.5mg/L 2,4-D and 1.0mg/L thiamine-HCl. Rice calli immersed in Agrobacterium suspension (strain EHA 105, OD(600)=0.8) were co-cultured on LS30-AsPC medium for 2 days at 25±2°C in the dark. Based on GUS expression analysis, 10min co-cultivation time with 100?M acetosyringone was found optimum for the delivery of gus gene. Calli were proved to be very sensitive to Agrobacterium infection and we found that the level of necrotic response can be minimized after co-cultivation with 30% LS, 10g/L PVP, 10% coconut water and 250mg/L timentin which improved the final transformation efficiency to 9.33%. Molecular and genetic analysis of transgenic plants reveals the integration, expression and inheritance of transgene in the progeny (T(1)) of these plants. The copy number of transgenes has been found to vary from 1 to 2 in transgenic plants (T(0) and T(1)). PMID:21763536

Karthikeyan, Alagarsamy; Pandian, Shunmugiah Karutha; Ramesh, Manikandan

2011-09-01

314

Molecular cloning and partial characterization of a peroxidase gene expressed in the roots of Portulaca oleracea cv., one potentially useful in the remediation of phenolic pollutants.  

PubMed

Portulaca (Portulaca oleracea cv.) efficiently removes phenolic pollutants from hydroponic solution. In plant roots, peroxidase (PRX) is thought to be involved in the removal of phenolic pollutants by the cross-linking them to cell wall polysaccharides or proteins at the expense of reduction of hydrogen peroxide (H(2)O(2)). In this study, we found that portulaca roots secreted an acidic PRX isozyme that had relatively high H(2)O(2) affinity. We isolated five PRX genes, and the recombinant PRX proteins produced in cultured tobacco cells were partially characterized. Among these genes, PoPRX2 probably encoded the acidic PRX isozyme. PoPRX2 had an extra N-terminal region which has not been reported for other PRX proteins. We found that PoPRX2 oxidized phenolic pollutants, including bisphenol A, octylphenol, nonylphenol, and 17?-estradiol. In addition, we found that the Cys261 residue of PoPRX2 played an important role in the determination of affinity for H(2)O(2) and stability toward H(2)O(2). PMID:21597193

Matsui, Takeshi; Nomura, Yuki; Takano, Mai; Imai, Sofue; Nakayama, Hideki; Miyasaka, Hitoshi; Okuhata, Hiroshi; Tanaka, Satoshi; Matsuura, Hideyuki; Harada, Kazuo; Bamba, Takeshi; Hirata, Kazumasa; Kato, Ko

2011-01-01

315

PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells  

PubMed Central

In an earlier study, we isolated potamin-1 (PT-1), a 5.6-kDa trypsin-chymotrypsin protease inhibitor, from the tubers of a potato strain (Solanum tuberosum L cv. Gogu Valley). We established that PT-1 strongly inhibits pathogenic microbial strains, but not human bacterial strains, and that its sequence shows 62% homology with a serine protease inhibitor. In the present study, we isolated an antifungal and antibacterial peptide with no cytotoxicity from tubers of the same potato strain. The peptide (peptide-G2, PG-2) was isolated using salt-extraction, ultrafiltration and reverse-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) showed the protein to have a molecular mass of 3228.5 Da, while automated Edman degradation showed the N-terminal sequence of PG-2 to be LVKDNPLDISPKQVQALCTDLVIRCMCCC-. PG-2 exhibited antimicrobial activity against Candida albicans, a human pathogenic yeast strain, and Clavibacter michiganensis subsp. michiganensis, a plant late blight strain. PG-2 also showed antibacterial activity against Staphylococcus aureus, but did not lyse human red blood cells and was thermostable. Overall, these results suggest PG-2 may be a good candidate to serve as a natural antimicrobial agent, agricultural pesticide and/or food additive. PMID:23429275

Kim, Jin-Young; Gopal, Ramamourthy; Kim, Sang Young; Seo, Chang Ho; Lee, Hyang Burm; Cheong, Hyeonsook; Park, Yoonkyung

2013-01-01

316

Characterization of Growth, Water Relations, and Proline Accumulation in Sodium Sulfate Tolerant Callus of Brassica napus L. cv Westar (Canola) 1  

PubMed Central

Unselected and sodium sulfate tolerant callus cultures of Brassica napus L. cv Westar were grown on media supplemented with mannitol, NaCl, or Na2SO4. In all cases, growth of tolerant callus, measured on a fresh weight or dry weight basis, was greater than that of unselected callus, which was also subject to necrosis on high levels of salt. Tissue water potential became more negative in both unselected and tolerant callus grown in the presence of mannitol or Na2SO4. Water potentials in unselected callus were more negative than those of the tolerant tissues; but over a range of Na2SO4 concentrations both cultures displayed osmotic adjustment, maintaining relatively constant turgor. Proline accumulation in both unselected and tolerant callus was low (15 to 20 micromoles per gram dry weight) in the absence of stress, but increased on media supplemented with mannitol, NaCl, or Na2SO4. Increases in proline concentration were approximately linear in tolerant callus, reaching a maximum of 130 to 175 micromoles per gram dry weight. In unselected callus, concentrations were higher, reaching 390 to 520 micromoles per gram dry weight. Proline accumulation was correlated with inhibition of growth, and there was a negative correlation between proline concentration and culture age for tolerant callus. PMID:16665381

Chandler, Stephen F.; Thorpe, Trevor A.

1987-01-01

317

The sequence of change within the photosynthetic apparatus of wheat following short-term exposure to ozone. [Triticum aestivum L. cv Avalon  

SciTech Connect

The basis of inhibition of photosynthesis by single acute O{sub 3} exposures was investigated in vivo using analyses based on leaf gas exchange measurements. The fully expanded second leaves of wheat plants (Triticum aestivum L. cv Avalon) were fumigated with either 200 or 400 nanomoles per mole O{sub 3} for between 4 and 16 hours. This reduced significantly the light-saturated rate of Co{sub 2} uptake and was accompanied by a parallel decrease in stomatal conductance. However, the stomatal limitation only increased significantly during the first 8 hours of exposure to 400 nanomoles per mole O{sub 3}; no significant increase occurred for any of the other treatments. Analysis of the response of CO{sub 2} uptake to the internal Co{sub 2} concentration implied that the predominant factor responsible for the reduction in light-saturated CO{sub 2} uptake was a decrease in the efficiency of carboxylation. At saturating concentrations of Co{sub 2}, photosynthesis was inhibited by no more than 22% after 16 hours, indicating that the capacity for regeneration of ribulose bisphosphate was less susceptible to O{sub 3}. Ozone fumigations also had a less pronounced effect on light-limited photosynthesis. The photochemical efficiency of photosystem II estimated from the ratio of variable to maximum chlorophyll fluorescence and the atrazine-binding capacity of isolated thylakoids demonstrated that photochemical reactions were not responsible for the initial inhibition of CO{sub 2} uptake.

Farage, P.K.; Long, S.P.; Baker, N.R. (Univ. of Essex (England)); Lechner, E.G. (Univ. of Vienna (Austria))

1991-02-01

318

Effect of boiling time on chemical composition and physico-functional properties of flours from taro (Colocasia esculenta cv fouê) corm grown in Côte d'Ivoire.  

PubMed

Taro (Colocasia esculenta cv fouê) corm was subjected to different boiling times and the changes in chemical composition and physico-functional properties were investigated using standard method. The change in boiling time led to a significant (P?

Amon, Anon Simplice; Soro, René Yadé; Assemand, Emma Fernande; Dué, Edmond Ahipo; Kouamé, Lucien Patrice

2014-05-01

319

Influence of Seismic Stress on Photosynthetic Productivity, Gas Exchange, and Leaf Diffusive Resistance of Glycine max (L.) Merrill cv Wells II 1  

PubMed Central

Relative growth rate (RGR), leaf water potential (?w), transpiration rate (Tr), photosynthetic rate (Pn), and stomatal and mesophyll resistances to CO2 exchange were measured or calculated to determine how periodic seismic (shaking) stress decreased dry weight accumulation by soybean (Glycine max [L.] Merrill cv Wells II). Seismic stress was applied with a gyratory shaker at 240 to 280 revolutions per minute for 5 minutes three times daily at 0930, 1430, and 1930 hours. Fifteen days of treatment decreased stem length 21%, leaf area 17%, and plant dry weight 18% relative to undisturbed plants. Seismic stress also decreased RGR 4%, which was due entirely to decreased net assimilation rate. Transpiration decreased 17% and leaf ?w increased 39% 30 minutes after treatment. A reduction in Pn began within seconds after the onset of treatment and had declined 16% after 20 minutes, at which time gradual recovery began. Assimilation rate recovered fully before the next seismic treatment 5 hours later. Resistance analysis and calculation of leaf internal CO2 levels indicated that the transitory decrease in Pn caused by periodic seismic stress was due to increased stomatal resistance on the abaxial leaf surface. Images Fig. 1 PMID:11540834

Pappas, Thalia; Mitchell, Cary A.

1985-01-01

320

Electrocatalytic activities of gold-5-amino-2-mercaptobenzimidazole-M n+ self-assembled monolayer complexes (M n+ : Ag +, Cu 2+) for hydroquinone oxidation investigated by CV and EIS  

Microsoft Academic Search

Electrocatalytic activity of a new catalyst toward the oxidation reaction of hydroquinone as a model compound is described. The catalyst was formed by immobilizing metal cations on the topside of a gold-5-amino-2-mercaptobenzimidazole, self-assembled monolayer (Au-5A2MBI-Mn+ SAM, Mn+: Cu2+, Ag+) electrode. Preparation steps and the electrocatalytic activity of the catalyst were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).

Reza Karimi Shervedani; Fatemeh Yaghoobi; Abdolhamid Hatefi-Mehrjardi; Saied Mohsen Siadat-Barzoki

2008-01-01

321

EFFECTS OF SINGLE, DUAL AND TRIPLE INOCULATIONS WITH BACILLUS SUBTILIS, BACILLUS MEGATERIUM AND RHIZOBIUM LEGUMINOSARUM BV. PHASEOLI ON NODULATION, NUTRIENT UPTAKE, YIELD AND YIELD PARAMETERS OF COMMON BEAN (PHASEOLUS VULGARIS L. CV. ‘ELKOCA-05’)  

Microsoft Academic Search

This study was conducted in order to investigate the effects of single, dual, and triple inoculations with Rhizobium, N2-fixing Bacillus subtilis (OSU-142), and P-solubilizing Bacillus megaterium (M-3) on nodulation, plant growth, nutrient uptake and seed yield of common bean (Phaseolus vulgaris L. cv. ‘Elkoca-05’) in comparison to control and mineral fertilizer application under field conditions in 2006 and 2007 in

Erdal Elkoca; Metin Turan; M. Figen Donmez

2010-01-01

322

Salicylic Acid Alleviates the Adverse Effects of Salt Stress in Torreya grandis cv. Merrillii Seedlings by Activating Photosynthesis and Enhancing Antioxidant Systems  

PubMed Central

Background Salt stress is a major factor limiting plant growth and productivity. Salicylic acid (SA) has been shown to ameliorate the adverse effects of environmental stress on plants. To investigate the protective role of SA in ameliorating salt stress on Torreya grandis (T. grandis) trees, a pot experiment was conducted to analyze the biomass, relative water content (RWC), chlorophyll content, net photosynthesis (Pn), gas exchange parameters, relative leakage conductivity (REC), malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD) and peroxidase (POD) of T. grandis under 0.2% and 0.4% NaCl conditions with and without SA. Methodology/Principal Findings The exposure of T. grandis seedlings to salt conditions resulted in reduced growth rates, which were associated with decreases in RWC and Pn and increases in REC and MDA content. The foliar application of SA effectively increased the chlorophyll (chl (a+b)) content, RWC, net CO2 assimilation rates (Pn), and proline content, enhanced the activities of SOD, CAT and POD, and minimized the increases in the REC and MDA content. These changes increased the capacity of T. grandis in acclimating to salt stress and thus increased the shoot and root dry matter. However, when the plants were under 0% and 0.2% NaCl stress, the dry mass of the shoots and roots did not differ significantly between SA-treated plants and control plants. Conclusions SA induced the salt tolerance and increased the biomass of T. grandis cv. by enhancing the chlorophyll content and activity of antioxidative enzymes, activating the photosynthetic process, and alleviating membrane injury. A better understanding about the effect of salt stress in T. grandis is vital, in order gain knowledge over expanding the plantations to various regions and also for the recovery of T. grandis species in the future. PMID:25302987

Du, Xuhua; Tang, Hui; Shen, Chaohua; Wu, Jiasheng

2014-01-01

323

Changes in pectin methyl esterase activity with different packaging materials and stages of fruit harvesting during cold storage of pear cv. Punjab beauty.  

PubMed

Pear cv. Punjab Beauty has become quite popular in Punjab. Excessive softening during cold storage leading to low shelf life is the major factor limiting its wider adoption. Studies were, therefore, conducted to determine the firmness and pectin methyl esterase (PME) activity at 4 harvest dates (2nd, 3rd and 4th week of July, and 1st week of August). Various packaging materials i.e. corrugated fiber board boxes and crates with high and low density polyethylene liners, corrugated fiber board boxes, crates and wooden boxes were also evaluated for their role in extending the shelf life of fruits. The enzyme activity and fruit firmness was evaluated periodically after 30, 45, 60 and 75 days of storage at 0-1 °C and 90-95 % RH. The firmness of the fruits decreased with the increase in storage intervals but the enzyme activity increased with the storage period up to 60 days and declined thereafter. Ripening-related changes in all the harvests were characterized mainly by an increase in the solubilization of pectin with a concomitant decrease in the degree of firmness. There was a continuous increase in enzyme activity with the advancement in harvesting dates and then fell sharply in the advanced ripening stages. Highest pectin methyl esterase activity was in fruits packed in crates followed by wooden boxes and corrugated fiber board boxes while the lowest was recorded in fruits packed in corrugated fiber board boxes with high density polyethylene liners. Therefore, high density polyethylene lined CFB boxes proved to be most effective in preventing the loss in firmness. PMID:25328240

Kaur, Kirandeep; Dhillon, W S; Mahajan, B V C

2014-10-01

324

Curdlan ?-1,3-Glucooligosaccharides Induce the Defense Responses against Phytophthora infestans Infection of Potato (Solanum tuberosum L. cv. McCain G1) Leaf Cells  

PubMed Central

Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H2O2 and salicylic acid and the activities of phenylalanine amino-lyase, ?-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually. PMID:24816730

Li, Jing; Zhu, Li; Lu, Guangxing; Zhan, Xiao-Bei; Lin, Chi-Chung; Zheng, Zhi-Yong

2014-01-01

325

QTL analysis for grain quality traits in 2 BC2F2 populations derived from crosses between Oryza sativa cv Swarna and 2 accessions of O. nivara.  

PubMed

The appearance and cooking quality of rice determine its acceptability and price to a large extent. Quantitative trait loci (QTLs) for 12 grain quality traits were mapped in 2 mapping populations derived from Oryza sativa cv Swarna × O. nivara. The BC(2)F(2) population of the cross Swarna × O. nivara IRGC81848 (population 1) was evaluated during 2005 and that from Swarna × O. nivara IRGC81832 (population 2) was evaluated during 2006. Linkage maps were constructed using 100 simple sequence repeat (SSR) markers in population 1 and 75 SSR markers in population 2. In all, 21 QTLs were identified in population 1 (43% from O. nivara) and 37 in population 2 (38% QTLs from O. nivara). The location of O. nivara-derived QTLs mp1.2 for milling percent, kw6.1 for kernel width, and klac12.1 for kernel length after cooking coincided in the 2 populations and appear to be useful for Marker Assisted Selection (MAS). Four QTLs for milling percent, 1 QTL each for amylose content, water uptake, elongation ratio, 2 QTLs for kernel width, and 3 QTLs for gel consistency, each explained more than 20% phenotypic variance. Three QTL clusters for grain quality traits were close to the genes/QTLs for shattering and seed dormancy. QTLs for 4 quality traits were associated with 5 of the 7 major yield QTLs reported in the same 2 mapping populations. Useful introgression lines have been developed for several agronomic traits. It emerges that 40% O. nivara alleles were trait enhancing in both populations, and QTLs for grain quality overlapped with yield meta-QTLs and QTLs for dormancy and seed shattering. PMID:22312119

Swamy, B P Mallikarjuna; Kaladhar, K; Shobha Rani, N; Prasad, G S V; Viraktamath, B C; Reddy, G Ashok; Sarla, N

2012-01-01

326

Increase in growth, productivity and nutritional status of wheat (Triticum aestivum L. cv. WH-711) and enrichment in soil fertility applied with organic matrix entrapped urea.  

PubMed

Field experiments were conducted during two consequent years in semi-arid, subtropical climate of Rohtak district situated in North-West Indian state Haryana to evaluate the effects of eco-friendly organic matrix entrapped urea (OMEU) on wheat (Triticum aestivum L. cv. WH-711). The OMEU prepared in granular form contained cow dung, rice bran (grain cover of Oryza sativa), neem (Azadirachta indica) leaves and clay soil (diameter of particles < 0.002 mm) in 1:1:1:1 ratios and saresh (plant gum of Acacia sp.) as binder entrapping half of the recommended dose of urea. A basal application of organic matrix entrapped urea showed increase in plant growth in terms of fresh and dry weights, root length, root number, leaf number, tillers, plant height earlet number, earlet length and productivity in terms of grain yield and straw yield over free form of urea (FU) and no fertilizer (NF) application. The OMEU increased total soluble proteins, organic N and free ammonium content in the leaves at 45 and 60 days. The nutritional status of wheat grains in OMEU applied plants was almost similar to that observed for FU applied plants. An increase in organic carbon and available phosphorus (P) was observed in OMEU applied plots on harvest whereas pH was slightly decreased over FU applied plots. The microbial population and activity in terms of fungal and bacterial colony count and activities soil dehydrogenase and alkaline phosphatase were significantly higher in OMEU applied plots as compared to the FU applied plots. Our data indicate that OMEU which are low cost, biodegradable and non-toxic can be used to replace the expensive chemical fertilizers for wheat cultivation in semi-arid, subtropical climate. PMID:24006800

Kumar, Manoj; Bauddh, Kuldeep; Kumar, Sanjeev; Sainger, Manish; Sainger, Poonam A; Singh, Rana P

2013-01-01

327

A Novel Late Embryogenesis Abundant Like Protein Associated with Chilling Stress in Nicotiana tabacum cv. Bright Yellow-2 Cell Suspension Culture*  

PubMed Central

Low temperature is one of the major abiotic stresses limiting the productivity and geographical distribution of many important crops. To identify proteins associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 (BY-2) cell suspension culture, we utilized a proteomic approach with two-dimensional electrophoresis to compare proteins from samples of treated with or without chilling treatment at 4 °C. One protein specifically more abundant in chilling treated sample was identified and designated as NtLEA7-3. Rapid amplification of cDNA ends gave rise to a full-length NtLEA7-3 cDNA with a complete open reading frame of 1267 bp, encoding a 322 amino acid polypeptide. Homology search and sequence multi-alignment demonstrated that the deduced NtLEA7-3 protein sequence shared a high identity with LEA-like proteins from other plants. Subcellular localization analysis indicated that the NtLEA7-3 was localized exclusively in the nucleus. When the gene was overexpressed in bright yellow-2 cells, the transgenic bright yellow-2 cells show more resistant to chilling stress than the wild-type cells. In addition, transgenic Arabidopsis plants overexpressing the NtLEA7-3 are much more resistant to cold, drought, and salt stresses. Interestingly, the expression of NtLEA7-3 in tobacco was not tissue-specific and induced by chilling, drought and salt stresses. All of these, taken together, suggest that NtLEA7-3 is worthwhile to elucidate the contribution of the proteins to the tolerance mechanism to chilling stress, and can be considered as a potential target for crop genetic improvement in the future. PMID:21653253

Gai, Ying-Ping; Ji, Xian-Ling; Lu, Wei; Han, Xue-Juan; Yang, Guo-Dong; Zheng, Cheng-Chao

2011-01-01

328

Effect of chitosan on tobacco mosaic virus (TMV) accumulation, hydrolase activity, and morphological abnormalities of the viral particles in leaves of N. tabacum L. cv. Samsun.  

PubMed

The effect of chitosan on the development of infection caused by Tobacco mosaic virus (TMV) in leaves of Nicotiana tabacum L. cv. Samsun has been studied. It was shown that the infectivity and viral coat protein content in leaves inoculated with a mixture of TMV (2 ?g/mL) and chitosan (1 mg/mL) were lower in the early period of infection (3 days after inoculation), by 63% and 66% respectively, than in leaves inoculated with TMV only. Treatment of leaves with chitosan 24 h before inoculation with TMV also caused the antiviral effects, but these were less apparent than when the virus and polysaccharide were applied simultaneously. The inhibitory effects of the agent decreased as the infection progressed. Inoculation of leaves with TMV together with chitosan considerably enhanced the activity of hydrolases (proteases, RNases) in the leaves, in comparison with leaves inoculated with TMV alone. Electron microscope assays of phosphotungstic acid (PTA)-stained suspensions from infected tobacco leaves showed that, in addition to the normal TMV particles (18 nm in diameter, 300 nm long), these suspensions contained abnormal (swollen, "thin" and "short") virions. The highest number of abnormal virions was found in suspensions from leaves inoculated with a mixture of TMV and chitosan. Immuno-electron microscopy showed that "thin" virus particles, in contrast to the particles of normal diameter, lost the ability to bind to specific antiserum. It seems that the chitosan-induced activation of hydrolases stimulates the intracellular degradation of TMV particles and hence hydrolase activation may be considered to be one of the polysaccharide-mediated cellular defense mechanisms that limit virus accumulation in cells. PMID:25116808

Nagorskaya, Vera; Reunov, Anatoliy; Lapshina, Larisa; Davydova, Viktoriya; Yermak, Irina

2014-08-01

329

Extracellular compounds produced by fungi associated with botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells.  

PubMed

Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. One hypothesis is that the extracellular compounds produced by the fungi associated with grapevine trunk diseases are responsible for pathogenicity.In the present work, we used Vitis vinifera cv. Chardonnay cells to test the aggressiveness of total extracellular compounds produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with botryosphaeria dieback. Additionally, the toxicity of purified mellein, a characteristic toxin present in the extracellular compounds of Botryosphaeriaceae, was assessed.Our results show that the total extracellular compounds produced by N. parvum induce more necrosis on Chardonnay calli and induce a different defence gene expression pattern than those of D. seriata. Mellein was produced by both fungi in amounts proportional to its aggressiveness. However, when purified mellein was added to the culture medium of calli, only a delayed necrosis and a lower-level expression of defence genes were observed. Extracellular compounds seem to be involved in the pathogenicity of the fungi associated with botryosphaeria dieback. However, the doses of mellein used in this study are 100 times higher than those found in the liquid fungal cultures: therefore, the possible function of this toxin is discussed. PMID:24752796

Ramírez-Suero, M; Bénard-Gellon, M; Chong, J; Laloue, H; Stempien, E; Abou-Mansour, E; Fontaine, F; Larignon, P; Mazet-Kieffer, F; Farine, S; Bertsch, C

2014-11-01

330

CV MATTEO VIALE INFORMAZIONI ANAGRAFICHE  

E-print Network

-Denis Diderot, Novembre 2005 - Gennaio 2007 Dottorando presso l'Universit`a Paris 7-Denis Diderot. TITOLI E DIPLOMI Dottorato in Logica Matematica Universit`a Paris 7-Denis Diderot e Universit`a di Torino`a Paris 7-Denis Diderot) Titolo della tesi: Applications of the proper forcing axiom to cardinal

Viale, Matteo

331

Nadia L. Zakamska CV Information  

E-print Network

development (sophomores, physics majors), JHU, Fall 2012 Galactic Structure and Stellar Dynamics (graduate, funded) Herschel (Sept 2011, funded) Gemini (GMOS, March 2010) Gemini (NIFS, March 2010) APO 3.5m (Triplespec, spring 2009, summer 2009) Gemini (GMOS, March 2006) Chandra (March 2005, funded) Spitzer (Nov

Weaver, Harold A. "Hal"

332

Bracken CV -1 Curriculum Vitae  

E-print Network

1998-2001 Graduate Research Fellow, National Science Foundation 1997-2000 Graduate Teaching Assistant, M.E.S. Bracken, M.H. Graham, and J.J. Stachowicz. in press. Plant- animal diversity relationships Series 459: 53-62. 20. Bracken, M.E.S. and N.H.N. Low* . 2012. Realistic losses of rare species

Brody, James P.

333

CV Sergienko 1 Curriculum Vitae  

E-print Network

on the ice-stream behavior. NASA mentor: Robert A. Bindschadler Summer school lecturer/instructor Advanced). Research assistant to the following projects: Information Technology Research Project in Climate Model sponsored, David E. Archer, P.I.), Collaborative Research on Earth's Largest Icebergs (NSF sponsored

Rodgers, Keith

334

CV Sergienko 1 Curriculum Vitae  

E-print Network

; the effect of subglacial hydrodynamics on the ice-stream behavior. NASA mentor: Robert A. Bindschadler Summer; Mathematical Modeling (in Paris). Research assistant to the following projects: Information Technology Research in the Equatorial Region (NSF sponsored, David E. Archer, P.I.), Collaborative Research on Earth's Largest Icebergs

Rodgers, Keith

335

CV Wenli Li Biotechnology and  

E-print Network

in Daphnia populations. $15,000. From the National Science Foundation, US. [DEB-1011419]. Li, W. 2005. 2011. Comparative and population genomic approaches reveal recent introns in Daphnia populations. PNAS. Lynch. 2009. Extensive, recent intron gains in Daphnia populations. Science. 326: 1260-1262. Li, W

336

CV Nils Christophersen October 2007  

E-print Network

and communication technology (ICT) to foster good governance and sustainable economic growth in developing countries partners focussed on the development and deployment of the open source health information system HISP (Health Information System Programme). Christophersen is now heading an initiative at the Department

Sahay, Sundeep

337

Curriculum vitae (CV) Susan Murcott  

E-print Network

the first compendium of arsenic contamination in water/food in the world, identifying site-specific arsenic contamination in 105 countries. o Safe drinking water via household and community-based water treatment & safe

Reuter, Martin

338

ROGER WIEGAND'S CV October 2011  

E-print Network

in Commutative Algebra and Algebraic Geometry (group infrastructure support­no salary). · NSA Grant 01G-144, $41 Conference (May 2005), $12,000. (A. Li and L. Avramov were the PIs, but I wrote much of the proposal.) · NSA

Logan, David

339

CV -Olivier Gagliardini Olivier GAGLIARDINI  

E-print Network

of the Institut Universitaire de France since 2010 Visiting scientist at UBC in Vancouver, Canada Background, Canada (2012-2013). Nominated at the Institut Universitaire de France (2010-2015) 2 years of full of a new teacher in 2007. Appointed member of the recruitment committe

Gagliardini, Olivier

340

CV Michael Schindelegger Curriculum Vitae  

E-print Network

. Vienna University of Technology (TU Vienna) Department of Geodesy and Geoinformation E120 University Assistant at TU Vienna, Department of Geodesy and Geoinformation (will end in December 2016) 10/2009 ­ 12/2012 Project Assistant at TU Vienna, Department of Geodesy and Geoinformation Project: `GGOS

Schuh, Harald

341

The Rhetoric of the CV  

ERIC Educational Resources Information Center

When a person sends in his or her job-application materials, he or she is not just assembling separate documents to fulfill the requirements of an ad. Those documents are part of a larger rhetorical whole, and together they form an argument for the viability of his or her candidacy for a particular job. Documents should be crafted meticulously,…

Eyler, Joshua R.

2012-01-01

342

Functional genomics of fuzzless-lintless mutant of Gossypium hirsutum L. cv. MCU5 reveal key genes and pathways involved in cotton fibre initiation and elongation  

PubMed Central

Background Fuzzless-lintless cotton mutants are considered to be the ideal material to understand the molecular mechanisms involved in fibre cell development. Although there are few reports on transcriptome and proteome analyses in cotton at fibre initiation and elongation stages, there is no comprehensive comparative transcriptome analysis of fibre-bearing and fuzzless-lintless cotton ovules covering fibre initiation to secondary cell wall (SCW) synthesis stages. In the present study, a comparative transcriptome analysis was carried out using G. hirsutum L. cv. MCU5 wild-type (WT) and it’s near isogenic fuzzless-lintless (fl) mutant at fibre initiation (0 dpa/days post anthesis), elongation (5, 10 and 15 dpa) and SCW synthesis (20 dpa) stages. Results Scanning electron microscopy study revealed the delay in the initiation of fibre cells and lack of any further development after 2 dpa in the fl mutant. Transcriptome analysis showed major down regulation of transcripts (90%) at fibre initiation and early elongation (5 dpa) stages in the fl mutant. Majority of the down regulated transcripts at fibre initiation stage in the fl mutant represent calcium and phytohormone mediated signal transduction pathways, biosynthesis of auxin and ethylene and stress responsive transcription factors (TFs). Further, transcripts involved in carbohydrate and lipid metabolisms, mitochondrial electron transport system (mETS) and cell wall loosening and elongation were highly down-regulated at fibre elongation stage (5–15 dpa) in the fl mutant. In addition, cellulose synthases and sucrose synthase C were down-regulated at SCW biosynthesis stage (15–20 dpa). Interestingly, some of the transcripts (~50%) involved in phytohormone signalling and stress responsive transcription factors that were up-regulated at fibre initiation stage in the WT were found to be up-regulated at much later stage (15 dpa) in fl mutant. Conclusions Comparative transcriptome analysis of WT and its near isogenic fl mutant revealed key genes and pathways involved at various stages of fibre development. Our data implicated the significant role of mitochondria mediated energy metabolism during fibre elongation process. The delayed expression of genes involved in phytohormone signalling and stress responsive TFs in the fl mutant suggests the need for a coordinated expression of regulatory mechanisms in fibre cell initiation and differentiation. PMID:23151214

2012-01-01

343

Phylogenetic Analysis of Microbial Diversity in the Rhizoplane of Oilseed Rape (Brassica napus cv. Westar) Employing Cultivation-Dependent and Cultivation-Independent Approaches.  

PubMed

The structure of the microbial rhizoplane community of the important crop plant oilseed rape was studied by using a culture-dependent as well as a culture-independent approach based on 16S rDNA amplification. After isolation of the microbial community from the rhizoplane of oilseed rape (Brassica napus cv. Westar), the collected suspension was divided into two parts. One part was used for cultivation of bacteria onto three different growth media to establish a culture collection. From the other part of the rhizoplane suspension, genomic DNA was isolated and purified. Thereafter, 16S rDNA was amplified by PCR and cloned to obtain a library of 16S rDNA genes representative for the bacterial communities of this habitat. Phylogenetic 16S rDNA sequence analysis of 103 clones of this library revealed considerable differences from the corresponding nucleotide sequences of 111 cultured bacteria. Whereas the 16S rDNA clone library was dominated by a-Proteobacteria and bacteria of the Cytophaga-Flavobacterium-Bacteroides (CFB) phylum (51% and 30%, respectively), less than 17% of the cultured bacteria belonged to these two groups. More than 64% of the cultivated isolates were allocated to the b- and g-subclasses of the Proteobacteria, which were present in the clone library at about 14%. Most of the clones of the a-Proteobacteria of the library showed highest similarity to Bradyrhizobium sp. No such bacteria were found in the culture collection. Similarly, the second dominant group of the clone library comprising members of the CFB phylum was represented in the culture collection by a single isolate. The phylogenetic analysis of isolates of the culture collection clearly emphasized the need to use different growth media for recovery of rhizoplane bacteria. Whereas most of the a-Proteobacteria were recovered on complex medium, most of the b-Proteobacteria were isolated onto minimal media. Our results demonstrate that the combined approach pursued in this paper is necessary to explore the biodiversity of bacterial rhizoplane communities. PMID:12024277

Kaiser, O.; Pühler, A.; Selbitschka, W.

2001-08-01

344

Growth, yield and quality attributes of a tropical potato variety (Solanum tuberosum L. cv Kufri chandramukhi) under ambient and elevated carbon dioxide and ozone and their interactions.  

PubMed

The present study was designed to study the growth and yield responses of a tropical potato variety (Solanum tuberosum L. cv. Kufri chandramukhi) to different levels of carbon dioxide (382 and 570ppm) and ozone (50 and 70ppb) in combinations using open top chambers (OTCs). Plants were exposed to three ozone levels in combination with ambient CO2 and two ozone levels at elevated CO2. Significant increments in leaf area and total biomass were observed under elevated CO2 in combination with ambient O3 (ECO2+AO3) and elevated O3 (ECO2+EO3), compared to the plants grown under ambient concentrations (ACO2+AO3). Yield measured as fresh weight of potato also increased significantly under ECO2+AO3 and ECO2+EO3. Yield, however, reduced under ambient (ACO2+AO3) and elevated ozone (ACO2+EO3) compared to ACO2 (filtered chamber). Number, fresh and dry weights of tubers of size 35-50mm and>50mm used for direct consumption and industrial purposes, respectively increased maximally under ECO2+AO3. Ambient as well as elevated levels of O3 negatively affected the growth parameters and yield mainly due to reductions in number and weight of tubers of sizes >35mm. The quality of potato tubers was also modified under different treatments. Starch content increased and K, Zn and Fe concentrations decreased under ECO2+AO3 and ECO2+EO3 compared to ACO2+AO3. Starch content reduced under ACO2+AO3 and ACO2+EO3 treatments compared to ACO2. These results clearly suggest that elevated CO2 has provided complete protection to ambient O3 as the potato yield was higher under ECO2+AO3 compared to ACO2. However, ambient CO2 is not enough to protect the plants under ambient O3 levels. Elevated CO2 also provided protection against elevated O3 by improving the yield. Quality of tubers is modified by both CO2 and O3, which have serious implications on human health at present and in future. PMID:24507140

Kumari, Sumita; Agrawal, Madhoolika

2014-03-01

345

Amoeboid olivine aggregates (AOAs) in the Efremovka, Leoville and Vigarano (CV3) chondrites: A record of condensate evolution in the solar nebula  

NASA Astrophysics Data System (ADS)

Amoeboid olivine aggregates (AOAs) in the Efremovka, Leoville, and Vigarano CV3 chondrites were studied using petrographic and microanalytical techniques to evaluate the origins of these inclusions and their relationships to other chondrite components. Our data support the idea that the inclusions formed by dust growth in the solar nebula as condensates and that they preserve a record of mineralogical and chemical evolution. Spinel-rich nodules and ribbons in AOAs often show Group II trace element patterns and formed either by fractional condensation or by condensation following partial vaporization. The dominant olivine component in AOAs can be explained as a condensate produced in the same way, but with fractionation occurring at a lower temperature. Other spinel-rich material in AOAs appears to be a vaporization residue. Ca-rich pyroxene + anorthite patches in AOAs show chemical signatures similar to those observed for spinel- or melilite-bearing regions but with lower refractory element and higher Mg and Si contents, supporting the idea that the patches formed by reaction between CAI-like material and Mg-Si-rich gas. Different trace element patterns (Group II and non-Group II refractory) are sometimes found for Al-rich regions (Ca-pyroxene + anorthite, or melilite-bearing) in a given AOA, suggesting that AOAs agglomerated from materials that formed under different conditions and that they did not subsequently homogenize. AOAs appear to have originated in a system with near-canonical solar composition and a low pressure gas (˜10-6-10-4 bar) over a range of temperatures (mainly ˜1200-1384 K) under somewhat non-equilibrium conditions. Relative to predicted equilibrium condensates, most AOAs show an apparent paucity of metal, possibly reflecting differential, density-dependent sorting between olivine and metal grains during aggregate formation. Trace element data are consistent with a simple model involving melting and igneous fractionation of AOA-like olivine to produce Type I chondrule olivine, but other data suggest that open-system melting of chondrules occurred in a different gaseous environment than that which produced AOAs.

Ruzicka, Alex; Floss, Christine; Hutson, Melinda

2012-02-01

346

Hypolipidemic effect of Goami-3 rice (Oryza sativa L. cv. Goami-3) on C57BL/6J mice is mediated by the regulation of peroxisome proliferator-activated receptor-? and -?.  

PubMed

We investigated the hypolipidemic effects of Goami-3 rice (GR; Oryza sativa L. cv. Goami-3), a newly developed strain with high levels of amylose and fibers. Diet-induced obese mice were fed three types of isocaloric diets for 8 weeks: a high-fat diet, a high-fat diet with GR or control rice (CR; O. sativa L. cv. Ilpumbyeo). Mice fed GR exhibited a significant reduction in body fat (-23%), total cholesterol (-20%) and triglyceride concentrations (-30%) compared to mice fed CR. The mice fed GR showed induction of peroxisome proliferator-activated receptor (PPAR)-? and inhibition of ? expressions in the liver and adipose tissue. The reduced adiposity of mice fed GC was supported by changes in the expression of genes related to lipid accumulation and hydrolysis in adipose tissues and the plasma concentrations of insulin, adiponectin and leptin. Principal components analysis with gas chromatography-time-of-flight mass spectrometry-based metabolomic data revealed that the average level of specific plasma metabolites in the GR group was statistically different from that in the other groups after 4weeks. These metabolites included propionic acid, valine, leucine and proline. Based on partial least-squares analysis, the plasma concentrations of valine were inversely correlated with the high-density lipoprotein (HDL) to non-HDL and HDL to total cholesterol ratios. In conclusion, GR feeding for 8 weeks significantly improved dyslipidemia and adiposity in diet-induced obese mice by regulating gene expression of PPARs and its target genes. Key plasma metabolites (including valine) were significantly altered by the hypolipidemic effects of GR. PMID:24075903

Lee, A-Young; Yeo, Siok-Koon; Lee, Ji Hae; Kim, Hea-won; Jia, Yaoyao; Hoang, Minh Hien; Chung, Hyun; Kim, Young-Suk; Lee, Sung-Joon

2013-11-01

347

High Efficiency Transformation of Banana [Musa acuminata L. cv. Matti (AA)] for Enhanced Tolerance to Salt and Drought Stress Through Overexpression of a Peanut Salinity-Induced Pathogenesis-Related Class 10 Protein.  

PubMed

Bananas and plantains (Musa spp. L.) are important subsistence crops and premium export commodity in several countries, and susceptible to a wide range of environmental and biotic stress conditions. Here, we report efficient, rapid, and reproducible Agrobacterium-mediated transformation and regeneration of an Indian niche cultivar of banana [M. acuminata cv. Matti (AA)]. Apical meristem-derived highly proliferative multiple shoot clump (MSC) explants were transformed with the Agrobacterium strain EHA105 harboring a binary vector pCAMBIA-1301 carrying hptII and uidA. Sequential agro-infiltration (10 min, 400 mmHg), infection (additional 35 min, Agrobacterium density A 600 = 0.8) and co-cultivation (18 h) regimen in 100 µM acetosyringone containing liquid medium were critical factors yielding high transformation efficiency (~81 %) corroborated by transient GUS expression assay. Stable transgenic events were recovered following two cycles of meristem initiation and selection on hygromycin containing medium. Histochemical GUS assay in several tissues of transgenic plants and molecular analyses confirmed stable integration and expression of transgene. The protocol described here allowed recovery of well-established putative transgenic plantlets in as little as 5 months. The transgenic banana plants could be readily acclimatized under greenhouse conditions, and were phenotypically similar to the wild-type untransformed control plants (WT). Transgenic plants overexpressing Salinity-Induced Pathogenesis-Related class 10 protein gene from Arachis hypogaea (AhSIPR10) in banana cv. Matti (AA) showed better photosynthetic efficiency and less membrane damage (P < 0.05) in the presence of NaCl and mannitol in comparison to WT plants suggesting the role of AhSIPR10 in better tolerance of salt stress and drought conditions. PMID:25173686

Rustagi, Anjana; Jain, Shalu; Kumar, Deepak; Shekhar, Shashi; Jain, Mukesh; Bhat, Vishnu; Sarin, Neera Bhalla

2015-01-01

348

Impact of the electrochemical porosity and chemical composition on the lithium ion exchange behavior of polypyrroles (ClO4-, TOS-, TFSI-) prepared electrochemically in propylene carbonate. comparative EQCM, EIS and CV studies.  

PubMed

Conditions of electrodeposition, i.e. a potential window of the process, addition of water, the current density, and morphology of substrate electrodes (Pt, Pt/TiO(2), Au), were shown to influence strongly ion-exchange properties of polypyrrole (PPy) synthesized in propylene carbonate (PC), doped with ClO(4)(-) or p-toluenesulfonate (TOS(-)). "Electrochemical porosity" and redox activity of PPy films were compared to the characteristics of poly(3,4-ethylenedioxythiophene) (PEDOT). A molecular indicator of the PPy film structure packing was bis(trifluoromethylsulfonyl)imide anion (TFSI(-)). Ion-exchange properties of PPy were found to be almost independent of chemical composition of the polymer, described in the literature as PPy(I), PPy(II), PPy(III). Instead, micro- and nanoscopic morphology of the polymer film and a molecular level packing of the polymer chains as well as the counterion nature are of the foremost importance. The polymer film structure/properties are shown to change upon prolonged redox/ionic stimulations. Lithium exchange between PPy films and contacting phases (PC electrolyte, TiO(2)) proceeds in addition to the anion exchange, the latter being a dominant process under conditions of the reversible electrochemical p-doping of PPy, although diffusion coefficients of PC solvated lithium ions in PPy are higher than diffusion coefficients of perchlorate, p-toluenesulfonate or bis(trifluoromethylsulfonyl)imide anions. The highest flux of Li(+) ions into/out of the PPy phase takes place about -1.0 V vs Ag/Ag(+) which is clearly evidenced by the cathodic/anodic CV peaks. Cation transport phenomena can be analyzed independently from anion transport when observed at a longer time scale (low values of potential scan rate) as each prevails at different redox states of the polymer. However, in a shorter time scale (v > or = 10 mV s(-1)), the opposite fluxes of cations and anions were observed to interfere. Furthermore, a net uptake of propylene carbonate by the as grown PPy film occurs at initial cycles of the cation uptake causing irreversible swelling of the polymer phase. Mechanisms of the redox process and accompanying mass transport involving PPy films were investigated using comparatively three techniques: cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and electrochemical quartz crystal microbalance (EQCM). PMID:20459080

Dziewo?ski, Pawe? Marek; Grzeszczuk, Maria

2010-06-01

349

Cloning and analysis of a cDNA encoding an endo-polygalacturonase expressed during the desiccation period of the silique-valves of turnip-tops (Brassica rapa L. cv. Rapa).  

PubMed

During zygotic embryogenesis of turnip-tops (Brassica rapa L. cv. Rapa), the polygalacturonase activity (PG; EC 3.2.1.15), measured as a decrease in viscosity of polygalacturonic acid, reached a high when the desiccation process in the seeded silique was triggered and the valves had lost more than 70-75% of their moisture (45-50 DPA). The PG activity was not detected in any phases of developing seeds. This work also characterizes a cDNA with an open reading frame of 1303 bp and that codes for a putative PG called BrPG1. This falls into the category of clade-B, which includes PG related to shattering and abscission processes. The deduced BrPG1 sequence predicted a 434-residue-long precursor protein (46.7kDa) with a transit peptide sequence 23 amino acids long. A molecular mass of 44.3 kDa was calculated for the mature form of BrPG1, which showed high sequence similarity to PGA1 (97%) of B. napus (X98373) and ADPG1 (87%) of Arabidopsis thaliana (AJ002532). All conserved amino acids at the catalytic site of PGs belonging to clade-B were preserved on BrPG1. This BrPG1 gene was specifically expressed in the silique valves of turnip-tops and was temporally expressed at the beginning of its desiccation. PMID:15022837

Rodríguez-Gacio, María del Carmen; Nicolás, Carlos; Matilla, Angel J

2004-02-01

350

Constitutive expression of CaXTH3, a hot pepper xyloglucan endotransglucosylase/hydrolase, enhanced tolerance to salt and drought stresses without phenotypic defects in tomato plants (Solanum lycopersicum cv. Dotaerang).  

PubMed

The hot pepper xyloglucan endo-trans-gluco-sylase/hydrolase (CaXTH3) gene that was inducible by a broad spectrum of abiotic stresses in hot pepper has been reported to enhance tolerance to drought and high salinity in transgenic Arabidopsis. To assess whether CaXTH3 is a practically useful target gene for improving the stress tolerance of crop plants, we ectopically over-expressed the full-length CaXTH3 cDNA in tomato (Solanum lycopersicum cv. Dotaerang) and found that the 35S:CaXTH3 transgenic tomato plants exhibited a markedly increased tolerance to salt and drought stresses. Transgenic tomato plants exposed to a salt stress of 100 mM NaCl retained the chlorophyll in their leaves and showed normal root elongation. They also remained green and unwithered following exposure to 2 weeks of dehydration. A high proportion of stomatal closures in 35S:CaXTH3 was likely to be conferred by increased cell-wall remodeling activity of CaXTH3 in guard cell, which may reduce transpirational water loss in response to dehydration stress. Despite this increased stress tolerance, the transgenic tomato plants showed no detectable phenotype defects, such as abnormal morphology and growth retardation, under normal growth conditions. These results raise the possibility that CaXTH3 gene is appropriate for application in genetic engineering strategies aimed at improving abiotic stress tolerance in agriculturally and economically valuable crop plants. PMID:21207033

Choi, Jun Young; Seo, Young Sam; Kim, Su Jin; Kim, Woo Taek; Shin, Jeong Sheop

2011-05-01

351

The effects on photosynthetic CO{sub 2} assimilation to long-term elevation of atmospheric CO{sub 2} concentration: An assessment of the response of Trifolium Repens L. cv. Blanca grown at F.A.C.E.  

SciTech Connect

Understanding how photosynthetic capacity acclimates to elevated CO{sub 2} concentrations is vital in predicting the response of important grassland species such as Trifolium repens. Previous studies of acclimatization have been carried out in artificial experimental conditions, such as acrylic greenhouses or controlled environment chambers. The advent of FACE technology has enabled a large area of crop to be fumigated in the field, providing more realistic growing conditions. Pure stands of Trifolium repens L. cv. Blanca grown at either 355 or 600{mu}mol mol{sup -1} CO{sub 2} were examined, and their photosynthetic response to elevated Ca determined via gas exchange studies. Rates of photosynthesis of young, fully expanded leaves were increased between 21 and 36% when grown and measured at elevated CO{sub 2}. This increase in A corresponded to a decrease in g{sub S} of between 18 and 52%. No acclimation effect was observed in the most frequently cut stands, whilst the response of stands clipped only 4 times per year was more variable. When down regulation of V{sub cmax} did occur, this was not nearly as marked as that which occurred in 3 other temperate species (Chrysanthemum leucanthemum, Ranunculus friesianus, Plantago lanceolata (L.) J. & C. Presl.), at similar growth regimes. No acclimation of stomatal frequency, SI or pore length was found to occur in the enriched clover stands.

Lewis, C.E.

1994-11-01

352

Effects of pH, sample size, and solvent partitioning on recovery of soluble phenolic acids and isoflavonoids in leaves and stems of red clover (Trifolium pratense cv. Kenland).  

PubMed

Several extraction parameters were tested to determine optimal conditions for extracting phenolics from leaves and stems of red clover (Trifolium pratense L. cv. Kenland), with the goal of using extracts in bioassays and in assessment of phenolic profiles. HPLC-UV profiles were compared before and after partitioning a methanolic extract of soluble phenolics with ethyl acetate-ethyl ether (1:1, v/v). The effect of extract pH on the partitioning of phenolics into the ethyl acetate-ethyl ether (EtOAc-Et2O) phase was evaluated, and several tissue weights were extracted to determine a minimum amount that could be extracted without loss of information. HPLC profiles of soluble phenolics were similar in the methanolic extracts and the partitioned EtOAc-Et2O extracts. However, recoveries in unpartitioned extracts were 2- to 4-fold greater than in the acidified, partitioned extracts. Also, recovery was considerably affected by the pH to which extracts were adjusted prior to partitioning. In extracts acidified to pH 2, recoveries were 2- to 7-fold higher than in extracts partitioned at pH 6. In extracts prepared from 250, 120, or 60 mg of tissue, peak areas of methanolic extracts were directly proportional to the amount of tissue extracted. PMID:22224282

Kagan, Isabelle A

2011-11-01

353

Chemopreventive effects of standardized ethanol extract from the aerial parts of Artemisia princeps Pampanini cv. Sajabal via NF-?B inactivation on colitis-associated colon tumorigenesis in mice.  

PubMed

Chronic inflammation is an underlying risk factor of colon cancer, and NF-?B plays a critical role in the development of inflammation-associated colon cancer in an AOM/DSS mouse model. The aim of this study was to determine whether the standardized ethanol extract obtained from the aerial parts of Artemisia princeps Pampanini cv. Sajabal (EAPP) is effective at preventing inflammation-associated colon cancer, and if so, to identify the signaling pathways involved. In the present study, protective efficacy of EAPP on tumor formation and the infiltrations of monocytes and macrophages in colons of an AOM/DSS mouse model were evaluated. It was found that colitis and tumor burdens showed statistically meaningful improvements after EAPP administration. Furthermore, these improvements were accompanied by a reduction in NF-?B activity and in the levels of NF-?B-dependent pro-survival proteins, that is, survivin, cFLIP, XIAP, and Bcl-2. In vitro, EAPP significantly reduced NF-?B activation and the levels of IL-1? and IL-8 mRNA and pro-survival proteins in HT-29 and HCT-116 colon cancer cells. Furthermore, EAPP caused caspase-dependent apoptosis. Based on these results, the authors suggest EAPP suppresses inflammatory responses and induces apoptosis partly via NF-?B inactivation, and that EAPP could be useful for the prevention of colitis-associated tumorigenesis. PMID:25449198

Chung, Kyung-Sook; Choi, Hye-Eun; Shin, Ji-Sun; Cho, Eu-Jin; Cho, Young-Wuk; Choi, Jung-Hye; Baek, Nam-In; Lee, Kyung-Tae

2015-01-01

354

Growth and Nutrient Uptake by Barley (Hordeum vulgare L. cv Herta): Studies Using an N-(2-Hydroxyethyl)ethylenedinitrilotriacetic Acid-Buffered Nutrient Solution Technique (II. Role of Zinc in the Uptake and Root Leakage of Mineral Nutrients).  

PubMed Central

Barley seedlings (Hordeum vulgare L. cv Herta) were grown in N-(2-hydroxyethyl)ethylenedinitrilotriacetic acid-buffered nutrient solutions with or without adequate Zn supplies. Fifteen-d-old Zn-deficient seedlings contained higher concentrations of Mn, Ca, Mg, and P in their shoots and more Fe, Mn, Cu, K, Ca, and P in their roots than did similar Zn-adequate seedlings, confirming results reported in our companion study (W.A. Norvell and R.M. Welch [1993] Plant Physiol 101: 619-625). Zn-deficient roots leaked greater quantities of K, Mn, Cu, and Cl than did roots supplied adequately with Zn; they also leaked significant amounts of Zn even though the seedlings were not supplied Zn during growth. Calculated uptake rates of P, Mn, and Na were sharply reduced, but uptake rates of K and Mg were stimulated by increasing the Zn2+ activity in nutrient solutions. Intact roots of Zn-deficient seedlings contained lower concentrations of 5,5[prime] -dithio-bis(2-nitrobenzoic acid) reactive sulfhydryl groups in comparison to Zn-adequate roots. Apparently, Zn is required for the uptake and retention of several mineral nutrients by roots, possibly by playing a protective role in preventing the oxidation of sulfhydryl groups to disulfides in root-cell plasma membrane proteins involved in ion channel-gating phenomena. PMID:12231718

Welch, R. M.; Norvell, W. A.

1993-01-01

355

Identification of betalains from petioles of differently colored Swiss chard (Beta vulgaris L. ssp. cicla [L.] Alef. Cv. Bright Lights) by high-performance liquid chromatography-electrospray ionization mass spectrometry.  

PubMed

The betalain pattern of differently colored Swiss chard (Beta vulgaris L. ssp. cicla [L.] Alef. cv. Bright Lights) was investigated for the first time. Nineteen betaxanthins and nine betacyanins were identified by RP-HPLC and positive ion electrospray mass spectrometry, co-injection experiments with semisynthetic reference compounds, and standards derived from authentic plant material, respectively. Histamine-betaxanthin and alanine-betaxanthin were found to be novel betaxanthins, which to the best of our knowledge have not been reported as natural compounds until now. Furthermore, tyramine-betaxanthin (miraxanthin III) and 3-methoxytyramine-betaxanthin, which to date were known only from families other than the Chenopodiaceae, were detected for the first time in colored Swiss chard. The betacyanin pattern of purple petioles was composed of betanin, isobetanin, betanidin, and isobetanidin. Although phyllocactin was present in only trace amounts, further acylated structures such as betanidin-monoferuloyl-5-O-beta-diglucoside and lampranthin II, accompanied by their corresponding C(15)-epimers, were identified. In addition, quantification of betalains and CIE LCh degrees measurements were performed with the colored extracts to correlate the visual appearance with the respective pigment patterns. Besides the novel phytochemical findings, the present study is useful for the evaluation of betalainic Swiss chard as a potential coloring foodstuff. PMID:15137842

Kugler, Florian; Stintzing, Florian C; Carle, Reinhold

2004-05-19

356

Carbon balance, partitioning and photosynthetic acclimation in fruit-bearing grapevine (Vitis vinifera L. cv. Tempranillo) grown under simulated climate change (elevated CO2, elevated temperature and moderate drought) scenarios in temperature gradient greenhouses.  

PubMed

Although plant performance under elevated CO2 has been extensively studied in the past little is known about photosynthetic performance changing simultaneously CO2, water availability and temperature conditions. Moreover, despite of its relevancy in crop responsiveness to elevated CO2 conditions, plant level C balance is a topic that, comparatively, has received little attention. In order to test responsiveness of grapevine photosynthetic apparatus to predicted climate change conditions, grapevine (Vitis vinifera L. cv. Tempranillo) fruit-bearing cuttings were exposed to different CO2 (elevated, 700ppm vs. ambient, ca. 400ppm), temperature (ambient vs. elevated, ambient +4°C) and irrigation levels (partial vs. full irrigation). Carbon balance was followed monitoring net photosynthesis (AN, C gain), respiration (RD) and photorespiration (RL) (C losses). Modification of environment (13)C isotopic composition (?(13)C) under elevated CO2 (from -10.30 to -24.93‰) enabled the further characterization of C partitioning into roots, cuttings, shoots, petioles, leaves, rachides and berries. Irrespective of irrigation level and temperature, exposure to elevated CO2 induced photosynthetic acclimation of plants. C/N imbalance reflected the inability of plants grown at 700ppm CO2 to develop strong C sinks. Partitioning of labeled C to storage organs (main stem and roots) did not avoid accumulation of labeled photoassimilates in leaves, affecting negatively Rubisco carboxylation activity. The study also revealed that, after 20 days of treatment, no oxidative damage to chlorophylls or carotenoids was observed, suggesting a protective role of CO2 either at current or elevated temperatures against the adverse effect of water stress. PMID:25462972

Salazar-Parra, Carolina; Aranjuelo, Iker; Pascual, Inmaculada; Erice, Gorka; Sanz-Sáez, Alvaro; Aguirreolea, Jone; Sánchez-Díaz, Manuel; Irigoyen, Juan José; Araus, José Luis; Morales, Fermín

2015-02-01

357

Salinity induces carbohydrate accumulation and sugar-regulated starch biosynthetic genes in tomato (Solanum lycopersicum L. cv. ‘Micro-Tom’) fruits in an ABA- and osmotic stress-independent manner  

PubMed Central

Salinity stress enhances sugar accumulation in tomato (Solanum lycopersicum) fruits. To elucidate the mechanisms underlying this phenomenon, the transport of carbohydrates into tomato fruits and the regulation of starch synthesis during fruit development in tomato plants cv. ‘Micro-Tom’ exposed to high levels of salinity stress were examined. Growth with 160 mM NaCl doubled starch accumulation in tomato fruits compared to control plants during the early stages of development, and soluble sugars increased as the fruit matured. Tracer analysis with 13C confirmed that elevated carbohydrate accumulation in fruits exposed to salinity stress was confined to the early development stages and did not occur after ripening. Salinity stress also up-regulated sucrose transporter expression in source leaves and increased activity of ADP-glucose pyrophosphorylase (AGPase) in fruits during the early development stages. The results indicate that salinity stress enhanced carbohydrate accumulation as starch during the early development stages and it is responsible for the increase in soluble sugars in ripe fruit. Quantitative RT-PCR analyses of salinity-stressed plants showed that the AGPase-encoding genes, AgpL1 and AgpS1 were up-regulated in developing fruits, and AgpL1 was obviously up-regulated by sugar at the transcriptional level but not by abscisic acid and osmotic stress. These results indicate AgpL1 and AgpS1 are involved in the promotion of starch biosynthesis under the salinity stress in ABA- and osmotic stress-independent manners. These two genes are differentially regulated at the transcriptional level, and AgpL1 is suggested to play a regulatory role in this event. PMID:19995825

Yin, Yong-Gen; Kobayashi, Yoshie; Sanuki, Atsuko; Kondo, Satoru; Fukuda, Naoya; Ezura, Hiroshi; Sugaya, Sumiko; Matsukura, Chiaki

2010-01-01

358

CV-SLAM using ceiling boundary  

Microsoft Academic Search

This paper deals with simultaneous localization and mapping(SLAM) problem for a mobile robot that travels around the indoor environments. A single camera looking up the ceiling is used as the only sensor. Line features are extracted from the boundaries between the ceiling and walls and parameterized for SLAM update. Extended Kalman Filter(EKF) is used for simultaneously estimating the current robot

Hyukdoo Choi; Dong Yeop Kim; Jae Pil Hwang; Euntai Kim; Young-Ouk Kim

2010-01-01

359

CV S. Judge 1 SHELLEY A. JUDGE  

E-print Network

. · Advisor: Dr. Doris Simonis B.S., Geology and History, Mount Union College, Alliance, Ohio, 1991 Laboratory, School of Earth Sciences, The Ohio State University, Gibraltar Island, Lake Erie, summer 2008 Geology, Earth History, Oceanography, Paleontology, Sedimentology and Stratigraphy, Seminar

Wilson, Mark A.

360

CV: Who Seung Lee Curriculum Vitae  

E-print Network

: Professors Pat Monaghan, Neil B. Metcalfe (Glasgow) and Marc Mangel (UCSC) 2002 ­ 2004 Kyung Hee University. Journal of Ornithology 151: 765-770 9. Lee WS, Monaghan P, Metcalfe NB. 2010. The trade-off between growth

361

Page 1 of 18 David Vine CV  

E-print Network

on Diego Garcia. In The War Machine and Global Health. Merrill Singer and G. Derrick Hodge, eds. Pp. 179 author, with Philip Harvey and S. Wojciech Sokolowski. In Waging War, Making Peace--Reparations and Human Rights. Barbara Rose Johnston and Susan Slyomovics, eds. Pp. 132-151. Walnut Creek, CA: Left Coast Press

Kim, Kiho

362

Jared W. Young, CV. CURRICULUM VITAE  

E-print Network

R21 GRANT2010-0843, "Visuospatial priming (VSP) in rats: Development of an animal model for Tourette Syndrome" 07/01/2010-06/30/2013: in NCE Co-Investigator: NIDA P50 DA026506-06, (Grant I PI), "Translational

Squire, Larry R.

363

CV DJ Stacchiola DARO J. STACCHIOLA  

E-print Network

Laboratory Building 555, Upton, NY 11973 Phone: (631) 344-4378 e-mail: djs@bnl.gov Fax: (631) 344-5815 Web: www.bnl.gov/chemistry/bio/StacchiolaDario.asp www.mtu.edu/chemistry/department/faculty/stacchiola/ Present Associate Chemist Position Chemistry Department, Brookhaven National Laboratory Upton, NY (2010

Homes, Christopher C.

364

CV (K. Jordan) 1 CURRICULM VITAE  

E-print Network

. L. (2011). Racial profiling in decisions to search: A preliminary analysis using propensity, G. E. (2009). Exploring the perceived extent of and citizens' support for consumer racial profiling of the racial gradient thesis. Journal of Crime and Justice. Jordan, K. L. (2012). Preventive detention and out

Wu, Shin-Tson

365

CV (K. Jordan) 1 CURRICULM VITAE  

E-print Network

-67. Higgins, G. E., Jennings, W. G., Jordan, K. L., & Gabbidon, S. L. (2011). Racial profiling in decisions' support for consumer racial profiling: Results from a national poll. Journal of Criminal Justice, 37/ethnicity on the perceived prevalence and support for racial profiling at airports. Criminal Justice Policy Review, 20

Van Stryland, Eric

366

Combustion of biomass and low CV fuels  

Microsoft Academic Search

This paper reviews the progress made in several areas concerning the combustion of biomass and low calorific value fuels at Cardiff over the last 20 years, including:• Efficient utilisation of low calorific value gases.• The use of cyclone combustors with poor quality solid fuels.• Pyrolysis processes and wastes.• Small efficient, well controlled, batch fed biomass stoves.Conclusions are drawn as to

T. ODoherty

2000-01-01

367

Emerging trends in CV flow visualization.  

PubMed

Blood flow patterns are closely linked to the morphology and function of the cardiovascular system. These patterns reflect the exceptional adaptability of the cardiovascular system to maintain normal blood circulation under a wide range of workloads. Accurate retrieval and display of flow-related information remains a challenge because of the processes involved in mapping the flow velocity fields within specific chambers of the heart. We review the potentials and pitfalls of current approaches for blood flow visualization, with an emphasis on acquisition, display, and analysis of multidirectional flow. This document is divided into 3 sections. First, we provide a descriptive outline of the relevant concepts in cardiac fluid mechanics, including the emergence of rotation in flow and the variables that delineate vortical structures. Second, we elaborate on the main methods developed to image and visualize multidirectional cardiovascular flow, which are mainly based on cardiac magnetic resonance, ultrasound Doppler, and contrast particle imaging velocimetry, with recommendations for developing dedicated imaging protocols. Finally, we discuss the potential clinical applications and technical challenges with suggestions for further investigations. PMID:22421178

Sengupta, Partho P; Pedrizzetti, Gianni; Kilner, Philip J; Kheradvar, Arash; Ebbers, Tino; Tonti, Giovanni; Fraser, Alan G; Narula, Jagat

2012-03-01

368

Thermoluminescence and Metamorphism in CV Chondrites  

Microsoft Academic Search

One of the effects of metamorphism in meteorites is the production of feldspar, a thermoluminescence (TL) phosphor, through the devitrification of primary chondrule glass [1]. The 105-fold variation in TL sensitivity among the ordinary chondrites reflects this process and has been used successfully to subdivide the petrographic type 3 meteorites into types 3.0-3.9 [2]. Although less pronounced, the variability exhibited

S. J. K. Symes; R. K. Guimon; P. H. Benoit; D. W. G. Sears

1993-01-01

369

CV: Thomas Flatt January 16, 2014 1  

E-print Network

.html http://www.unil.ch/dee/page95005.html POSITIONS 2012-date: SNF Professor (Swiss National Science Science Foundation (SNF) Professorship 2011-12: Fellow, Institute for Advanced Study Berlin of Lifespan Modulation by Reproduction and Diet, Grant # 13C154. Duration: 12 months. CHF 60,000. 2012-: SNF

Lehmann, Laurent

370

CV: Thomas Flatt September 3, 2013 1  

E-print Network

. Thomas Flatt SNF Professor Department of Ecology and Evolution University of Lausanne UNIL Sorge in Zoology, Specialization in Population Biology). PROFESSIONAL EXPERIENCE AND POSITIONS HELD 2012-date: SNF, University of Basel, Switzerland. HONORS AND AWARDS 2012-date: Swiss National Science Foundation (SNF

Lehmann, Laurent

371

New Bright CV detected by MASTER  

NASA Astrophysics Data System (ADS)

MASTER-Amur auto-detection system discovered OT source at (RA, Dec) = 05h 15m 29.15s +56d 05m 32.0s on 2013-09-27.71853 UT. The OT unfiltered magnitude is 14.1m (limit 16.7m). The OT is seen in 14 images. There is no minor planet at this place. We have reference image without OT on 2012-10-19.72941 UT with unfiltered magnitude limit 18.6m.

Balanutsa, P.; Denisenko, D.; Gorbovskoy, E.; Lipunov, V.; Tiurina, N.; Kornilov, V.; Belinski, A.; Shatskiy, N.; Chazov, V.; Kuznetsov, A.; Yecheistov, V.; Vladimirov, V.; Yurkov, V.; Sergienko, Y.; Varda, D.; Sinyakov, E.; Gabovich, A.; Ivanov, K.; Yazev, S.; Budnev, N.; Konstantinov, E.; Chuvalaev, O.; Poleshchuk, V.; Gress, O.; Frolova, A.; Parkhomenko, A.; Tlatov, A.; Dormidontov, D.; Senik, V.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Podvorotny, P.; Shumkov, V.; Shurpakov, S.; Levato, H.; Saffe, C.; Mallamaci, C.; Lopez, C.; Podest, F.

2013-09-01

372

JEREMY HEIS CV, updated September 2012  

E-print Network

for the History of Philosophy 19 (2011): 759-94. (This paper received the 2011 Rogers Prize from the British of Mathematics: Modern Essays. Vol.1: The Critical Philosophy and Its Background, edited by Ofra Rechter and Carl

Loudon, Catherine

373

BrMYB4, a Suppressor of Genes for Phenylpropanoid and Anthocyanin Biosynthesis, is Down-Regulated by UV-B but not by Pigment-Inducing Sunlight in Turnip cv. Tsuda.  

PubMed

The regulation of light-dependent anthocyanin biosynthesis in Brassica rapa subsp. rapa cv. Tsuda turnip was investigated using an ethyl methanesulfonate (EMS)-induced mutant R30 with light-independent pigmentation. TILLING (targeting induced local lesions in genomes) and subsequent analysis showed that a stop codon was inserted in the R2R3-MYB transcription factor gene BrMYB4 and that the encoded protein (BrMYB4mu) had lost its C-terminal region. In R30, anthocyanin accumulated in the below-ground portion of the storage root of 2-month-old plants. In 4-day-old seedlings and 2-month-old plants, expression of BrMYB4 was similar between R30 and the wild type (WT), but the expression of the cinnamate 4-hydroxylase gene (BrC4H) was markedly enhanced in R30 in the dark. In turnip seedlings, BrMYB4 expression was suppressed by UV-B irradiation in the WT, but this negative regulation was absent in R30. Concomitantly, BrC4H was repressed by UV-B irradiation in the WT, but stayed at high levels in R30. A gel-shift assay revealed that BrMYB4 could directly bind to the promoter region of BrC4H, but BrMYB4mu could not. The BrMYB4-enhanced green fluorescent protein (eGFP) protein could enter the nucleus in the presence of BrSAD2 (an importin ?-like protein) nuclear transporter, but BrMYB4mu-eGFP could not. These results showed that BrMYB4 functions as a negative transcriptional regulator of BrC4H and mediates UV-B-dependent phenylpropanoid biosynthesis, while BrMYB4mu has lost this function. In the storage roots, the expression of anthocyanin biosynthesis genes was enhanced in R30 in the dark and in sunlight in both the WT and R30. However, in the WT, anthocyanin-inducing sunlight did not suppress BrMYB4 expression. Therefore, sunlight-induced anthocyanin biosynthesis does not seem to be regulated by BrMYB4. PMID:25305244

Zhang, Lili; Wang, Yu; Sun, Mei; Wang, Jing; Kawabata, Saneyuki; Li, Yuhua

2014-12-01

374

Graduate Council Meeting Agenda 310 Jack K. Williams Administration Building  

E-print Network

Nuclear Terrorism Threat Assessment and Analysis l. INTA 672 East Asian Security (tabled in March 2013) m in the Persian Gulf (tabled in March 2013) o. INTA 675 Religion and Politics in Iran (tabled in March 2013) p Policy in the Persian Gulf l. INTA 675 Religion and Politics in Iran m. INTA 676 International Politics

375

Yerslt:V of IHaho _9;~cv ~~W~cv~  

E-print Network

beads· ..GHJ: The Garden banenders and bouncers were just a few of the people that kept Mardi Gras hoppln·. #12;un: Drinks all around! Moscow Mardi Gras brlnt}s people tot}ether to party the nlt}ht away. un: Danclne, soclallz- lne, drlnklnt all add up to the perfect Moscow rendition of Mardi Gras. un

O'Laughlin, Jay

376

Graduate Council Meeting Agenda 310 Jack K. Williams Administration Building  

E-print Network

Nuclear Terrorism Threat Assessment and Analysis l. INTA 672 East Asian Security (tabled in March 2013) m in the Persian Gulf (tabled in March 2013) o. INTA 675 Religion and Politics in Iran (tabled in March 2013) p

377

Graduate Council Meeting Agenda 204A Evans Library  

E-print Network

Civil Wars (tabled in March 2013) k. INTA 669 Nuclear Terrorism Threat Assessment and Analysis l. INTA 675 Religion and Politics in Iran (tabled in March 2013) p. INTA 676 International Politics, and Procedures (tabled in March 2013) r. MARB 610 Professional Development s. NUEN 669 Nuclear Terrorism Threat

378

Graduate Council Meeting Minutes 310 Jack K. Williams Administration Building  

E-print Network

Civil Wars (tabled in March 2013) k. INTA 669 Nuclear Terrorism Threat Assessment and Analysis l. INTA 675 Religion and Politics in Iran (tabled in March 2013) p. INTA 676 International Politics, and Procedures (tabled in March 2013) r. MARB 610 Professional Development s. NUEN 669 Nuclear Terrorism Threat

379

BRIEF CURRICULUM VITAE (Full CV available on request)  

E-print Network

Government Commissioner for Administrative Reform, 1982-1983 Dean, Faculty of Social and Behavioural Sciences Award for teaching excellence (Faculty of Social and Behavioural Sciences, Leiden University) 2005 Shortlisted for the 2011 Huibregtsen Award for research that is innovative and has potential for social

Galis, Frietson

380

Eastman CV--2-4-14 CURRICULUM VITAE  

E-print Network

such as altruism and competitiveness as manifested in business ethics, political ideologies, and other beliefs Business Ethics," my recently published paper in Business Ethics Quarterly, the top academic journal with Palgrave Macmillan, Why Business Ethics Matters: The Logic of Moral Emotions in Game Theory, brings

Lin, Xiaodong

381

CV, T.S. Hopkins, 2009 Thomas Sawyer HOPKINS  

E-print Network

, IT 2002 ­ Faculty Federico II Univ. Naples, Doctorate in Marine Science and Engineering 2003 - Scientific for the Coastal Marine Environment Calata Porta di Massa n. 80 Porto di Napoli, 80133 Naples, IT Tel: +39 081 542-62 Research and Teaching Assist, Univ. Wash. 1965-70 Instructor, Univ. Wash. 1968-69 Partner, Marine Repair

Parker, Matthew D. Brown

382

CV McPhee-Shaw -1 Curriculum Vitae  

E-print Network

, University of California Santa Barbara 1993 ­ 2000 Graduate Research Assistant. School of Oceanography oceanography representative on the Monterey Bay National Marine Sanctuary's Research Activity Panel. 2006- 2013. Peer reviewer: Deep-Sea Research, Journal of Geophysical Research, Limnology and Oceanography, National

McPhee-Shaw, Erika

383

CV and List of Publications Emmanouil C. Zacharenakis  

E-print Network

, French. 3. Diploma in Civil Engineering, Aristotle University of Thessaloniki, Greece, 1966- 1971. 4. Civil Engineering in Military Services (1972 - 1974) and in Prefecture of Heraklion Crete (1975-1977). 5D in Civil Engineering, Aristotle University, Thessaloniki, Greece, 1991. Supervising professor, P. D

Stavroulakis, Georgios E.

384

Deborah Lawrence, CV January 2013 1 DEBORAH LAWRENCE  

E-print Network

1989 Harvard University, Biological Anthropology, Magna Cum Laude PROFESSIONAL EXPERIENCE 2012-present) Peer-reviewed journal articles 1. *Tully, K., T. Wood, A. Schwantes and D. Lawrence. In press In press. Physical and Biological Feedbacks of Deforestation. Reviews of Geophysics. 4. *Tully, K., and D

Lawrence, Deborah

385

CV Stockli 9/2012 CURRICULUM VITAE -Daniel F. Stockli  

E-print Network

and Maturation of Paleozoic strata of the Western Desert, Egypt. $149,972. 2009 NSF Tectonics. Collaborative of Technology (ETH), Zurich. Thesis: "Tectonics SW of the Tauern Window, Eastern Alps - Southern Continuation-temperature thermochronological and neotectonic constraints from the Middle Magdalena Valley, Llanos basin, and Eastern Cordillera

Yang, Zong-Liang

386

CV of Marco Di Francesco Updated November 2013  

E-print Network

hyperbolic conservation laws. Compressible gas-dynamics models. Diffusive relaxation limits for hyperbolic at the University of Rome "Tor Vergata". PhD Thesis: Diffusive behaviour and asymptotic self similarity for fluid's with nonlocal interactions with applications to aggregative phenomena in biology and social sciences. Nonlinear

Di Francesco, Marco

387

CV of Marco Di Francesco Updated May 2013  

E-print Network

. · Nonlinear hyperbolic conservation laws. · Compressible gas-dynamics models. · Diffusive relaxation limits at the University of Rome "Tor Vergata". PhD Thesis: Diffusive behaviour and asymptotic self similarity for fluid's with nonlocal interactions with applications to aggregative phenomena in biology and social sciences

Di Francesco, Marco

388

Dan McCarthy's CV 1 CURRICULUM VITAE  

E-print Network

ABSTRACTS Vision Sciences Society Meeting, St. Pete Beach, FL 2014 McCarthy, J. D., Barnes, L. N information via color. Vision Sciences Society Meeting, Naples, FL 2013 McCarthy, J. D. & Caplovitz, G. P Neuroscience Society Meeting, San Francisco, CA 2013 McCarthy, J. D., Barnes, L. N. & Caplovitz, G. C. Two plus

Song, Joo-Hyun

389

RESISTANCE OF TULARE WALNUT (JUGLANS REGIA CV. TULARE) TO AFLATOXIGENESIS  

Technology Transfer Automated Retrieval System (TEKTRAN)

The ability of tree nuts to inhibit production of aflatoxins by Aspergillus flavus was investigated in vitro. Walnuts were much more resistant to aflatoxin formation than almonds or pistachios. The walnut cultivar 'Tulare' showed complete suppression of toxin production. Kernel without seed coat ...

390

A.T. Kramer CV 1 Andrea T. Kramer  

E-print Network

: Conservation Scientist, Ecological Genetics, Chicago Botanic Garden 2012 ­ Present: Adjunct Assistant Professor to support ex situ conservation of genetic diversity in Magnolia, a flagship group. Biodiversity.F. Howe. 2008. The paradox of forest fragmentation genetics. Conservation Biology 22(4): 878 - 885. Kramer

Fant, Jeremie

391

Jason Aughenbaugh: CV Page 1 JASON MATTHEW AUGHENBAUGH  

E-print Network

fusion in sonar systems. As a graduate student, I played a large role in establishing my Ph.D. advisor ­ Aug. 2006 Graduate Independent Work Special Problems Course, Georgia Institute of Technology - Studied

392

Burlando CV--April 2014 1 Alfredo Burlando  

E-print Network

Walker Pedersen and Jessica Goldberg) "Prohibition, Legalization, and Police Corruption" (with Alberto FIELDS OF INTEREST Development, Health, Economics of Corruption, Savings RELEVANT WORK EXPERIENCE 2010: Optimal Enforcement Policies for Corruptible Officials." (with Alberto Motta). Invited revise and resubmit

Oregon, University of

393

Lawrence CV 1 Shawn A. Lawrence, PhD, LCSW  

E-print Network

Intervention (ETI) program. School Social Work Journal 35(2), 57-71. Lawrence, S. A., & Hazlett, R. & Mazur's adherence to medication and treatment. Journal of Human Behavior in the Social Environment, 19(7), 859: slawrence15@cfl.rr.com Current Position: 8/2005-Present Associate Professor (Promoted Fall 2011) University

Van Stryland, Eric

394

Lawrence CV 1 Shawn A. Lawrence, PhD, LCSW  

E-print Network

of the Early Truancy Intervention (ETI) program. School Social Work Journal, 35(2), 57-71. Lawrence, S. A's adherence to medication and treatment. Journal of Human Behavior in the Social Environment, 19(7), 859.lawrence@ucf.edu Employment: Current Position: 5/2013-Present Interim MSW Program Coordinator 8/2005-Present Associate

Wu, Shin-Tson

395

ES9 NPP-FM5 Ed1-CV  

Atmospheric Science Data Center

... Detailed CERES ERBElike Level 3 (ES-4/ES-9) Product Information Collection Guide:  ES9 CG R1V1  (PDF) Data ... for instrument validation purposes only and not suited for science publications. SCAR-B Block:  ...

2015-01-28

396

ES8 NPP-FM5 Ed1-CV  

Atmospheric Science Data Center

... Detailed CERES ERBElike Level 2 (ES-8) Product Information Collection Guide:  ES8 CG R3V4  (PDF) Data ... for instrument validation purposes only and not suited for science publications. SCAR-B Block:  ...

2015-01-28

397

ES4 NPP-FM5 Ed1-CV  

Atmospheric Science Data Center

... Detailed CERES ERBElike Level 3 (ES-4/ES-9) Product Information Collection Guide:  ES4 CG R1V1  (PDF) Data ... for instrument validation purposes only and not suited for science publications. Additional Info:  f SCAR-B ...

2015-01-28

398

Nash CV 1 of 4 Dwayne Abdul Nash, Esq.  

E-print Network

American Studies and Performance Studies Dissertation Title: "Prototypes of Racial Profiling: Forgotten Beautiful Struggle," Northwestern University, Evanston, IL "Forbidden Testimonies of Racial Profiling

399

LYNN STEPHEN October 30, 2010 C.V.  

E-print Network

Development and Gender Roles in Zapotec Ritual and Production. Carleton College, B.A. in Anthropology; qualitative methods; comparative Latino/a and Latin American Studies; ethnicity; gender and sexuality; social. RESEARCH AREAS: claiming rights (human, indigenous, women's), political identities; gender; ethnic identity

Oregon, University of

400

Dantzer CV Page 1/6 DR. BEN DANTZER A  

E-print Network

of Biology, GPA = 3.76/4.0 Thesis title: Behavioral Ecology of Red-backed Salamanders in Northern Michigan Volatile Chemical Signals in Red-backed Salamanders Advisors: Dr. Robert G. Jaeger & Dr. Brad R. Moon BSc. Northern Michigan University, Marquette, MI 2000-2004 BSc. In Biology with Ecology Emphasis, Department

Dantzer, Ben

401

Twenty-Eight Years of CV Results With the AAVSO  

NASA Astrophysics Data System (ADS)

Working with AAVSO data and AAVSO staff on cataclysmic variables since the 1980s resulted in twenty-nine papers from 1984 to 2011. The early work began with characterization of optical light curves of various dwarf novae and novalikes, then moved into coordination of optical observations with satellites (IUE, EUVE, XMM, Chandra, HST, GALEX) to explore the ultraviolet and X-ray regimes of disk systems versus those containing magnetic white dwarfs. The major advances in the field that were derived from these results are summarized, ending with the recent results on the cooling of the white dwarfs and the return of pulsations in GW Lib and V455 And following their 2007 outbursts, and on the spectra of the two peculiar Z Cam systems IW And and V513 Cas.

Szkody, P.; Mukadam, A. S.; Gaensicke, B. T.; Mattei, J. A.; Henden, A. H.; Simonsen, M.; Templeton, M. R.; Waagen, E. O.; Walker, G.; Sion, E. M.; Howell, S. B.; Townsley, D.

2012-06-01

402

Shengli Chen, CV 1/5 Shengli Chen  

E-print Network

), The University of Oklahoma December 2012 Dissertation: Analytical and numerical analyses of wellbore drilled, China June 2000 Dissertation: Vertical vibrations of foundations on saturated grounds B. S. Civil Engineering Assistant Professor Louisiana State University 08/2014-present Aramco Research Center Research

Cai, Steve C. S.

403

CV Thomas Knpfel, October 2011 Page 1 of 28  

E-print Network

journals, > 5500 citations, H factor of 43 (http://www.researcherid.com/rid/B-1128-2008). Numerous book to combine calcium measurements using fluorescent indicators with intracellular electrode recordings to study

Fukai, Tomoki

404

J.S. Karst CV 1 Jeffrey S. Karst  

E-print Network

syndrome and high functioning autism. Children's Hospital of Wisconsin: Hematology and assessments and therapy services with a broad range of children and adults. Therapies include individual psychotherapy, couples counseling, and group therapy drawing

Sanders, Matthew

405

CV of Monica Pokorny page 1 MONICA L. POKORNY  

E-print Network

: 4065708010 email: monicapokorny@gmail.com EDUCATION M.S. 2002, Land Resources and Environmental Sciences on the Sweetwater River in southcentral Wyoming. Included completion of a Prospectus and Mitigation Bank Instrument to quantify water movement into and through the ash pond cap system. Wildlife Habitat Restoration Ecologist

Lawrence, Rick L.

406

Elizabeth Frank CV 1 CU-Boulder, UCB 399  

E-print Network

assistant to Prof. Bruce Watson (Dept. Earth & Env. Sci.), I quantified sulfur diffusion through pyrite, and E.A. Frank (2009) Retention of biosignatures and mass- independent fractionations in pyrite: Self

Mojzsis, Stephen J.

407

C.V. Kunimoto August 2012 1 Namiko Kunimoto  

E-print Network

of British Columbia Art History, Visual Art and Theory, 2001 Intimate Archives: Japanese-Canadian Family" Japanese-Canadian National Museum, Vancouver May 2001 "A Place of Refuge: Japanese-Canadian Family's Normative Time Award 2008 Japan Foundation Research

Carlini, David

408

PAVEL MRAZEK CV 03/2005 Personal details  

E-print Network

Transactions on Medical Imaging, Signal Processing, Computational Statistics & Data Analysis, International University, Germany research topics: relations between nonlinear filters in digital image processing 2003, DAGM 2003, VLSM 2003, and for the journals IEEE Transactions on Image Processing, IEEE

409

Near-infrared face recognition utilizing open CV software  

NASA Astrophysics Data System (ADS)

Commercially available hardware, freely available algorithms, and authors' developed software are synergized successfully to detect and recognize subjects in an environment without visible light. This project integrates three major components: an illumination device operating in near infrared (NIR) spectrum, a NIR capable camera and a software algorithm capable of performing image manipulation, facial detection and recognition. Focusing our efforts in the near infrared spectrum allows the low budget system to operate covertly while still allowing for accurate face recognition. In doing so a valuable function has been developed which presents potential benefits in future civilian and military security and surveillance operations.

Sellami, Louiza; Ngo, Hau; Fowler, Chris J.; Kearney, Liam M.

2014-06-01

410

NASA/ESA CV-990 Spacelab Simulation (ASSESS 2)  

NASA Technical Reports Server (NTRS)

Cost effective techniques for addressing management and operational activities on Spacelab were identified and analyzed during a ten day NASA-ESA cooperative mission with payload and flight responsibilities handled by the organization assigned for early Spacelabs. Topics discussed include: (1) management concepts and interface relationships; (2) experiment selection; (3) hardware development; (4) payload integration and checkout; (5) selection and training of mission specialists and payload specialists; (6) mission control center/payload operations control center interactions with ground and flight problems; (7) real time interaction during flight between principal investigators and the mission specialist/payload specialist flight crew; and (8) retrieval of scientific data and its analysis.

1977-01-01

411

Susan Blake CV 1 SUSAN BLAKE CURRICULUM VITAE  

E-print Network

a focus on Classical Chinese language and philosophy). Expected degree date: Summer 2015. University of Illinois at Urbana-Champaign, Philosophy PhD program, 2000--2006. MA conferred May 2003. International Center Travel Grant to Eastern APA, December 2012. Summer Fellowship, University of Illinois at Urbana

Indiana University

412

CV of Reiner Hartenstein http://hartenstein.de/keys.html  

E-print Network

by the DoD. Introducing universities to VLSI systems design. In the early 80ies Reiner Hartenstein has also and industrial research funding, received several awards, and consulted for more than 10 international companies is professor emeritus1 of Computer Science (Informatik) at Kaiserslautern University of Technology (TU

Hartenstein, Reiner

413

Role of chlorophyllase in chlorophyll metabolism in olives cv. Gordal  

Microsoft Academic Search

During the development of the olive two stages in chlorophyll evolution can be distinguished: one of synthesis when the fruit is in the growth phase, and a degradative stage which begins when the fruit is completely developed. Although the enzyme chlorophyllase is present in olives throughout their development cycle, its activity reaches a maximum at both the beginning and end

Ma. Isabel Minguez-Mosquera; Lourdes Gallardo-Guerrero

1996-01-01

414

1 KS.Inglett_CV KANIKA SHARMA INGLETT  

E-print Network

production and oxidation. Aquatic food webs to study the trophic interactions between microbial functional the bacterial metabolic products with high pressure liquid chromatography (HPLC), and UV spectroscopy

Jawitz, James W.

415

Boris Faybishenko's CV January 25, 2010 BORIS FAYBISHENKO  

E-print Network

--Chernobyl Exclusion Zone, Ukraine; Ezeiza Nuclear Waste Disposal Site, Argentina, and Russia--Mayak, Lake Karachay Vadose Zone Processes and Environmental Remediation, Leader. PI and Co-PI of several DOE projects, Department of Civil and Environmental Engineering (1998), Graduate course Vadose Zone Hydrology; Kiev State

Ajo-Franklin, Jonathan

416

S. Klosterhaus, CV, p. 1 of 3 SUSAN L. KLOSTERHAUS  

E-print Network

and North American Brominated Flame Retardant Annual Meetings (2004-2010) Society of Environmental, CA. 49 pp. Davis, E.F., Klosterhaus, S., Stapleton, H.M. 2011. Measurement of flame retardants (invited, 2008) The Fire Retardant Dilemma Symposium (UC Berkeley) (invited, 2007, 2008) International

417

Yang's CV: 1 of 72 Department of Atmospheric Sciences  

E-print Network

· Certificate of Appreciation, National Institute of Standards and Technology (NIST), March 2011. · Certificate Achievement Award to CRYSTAL-FACE Science Team, 2003 · Best Paper Award, Climate and Radiation Branch, NASA

418

Belsky, CV October 2013, p. 1 JILL M. BELSKY  

E-print Network

economy, and often conducted with ecological scientists, my research (conducted both in the U.S Social Theory, Cultural and Biological Diversity RESEARCH FOCUS: My research focuses. intermountain west and overseas) strives to provide critical analyses to foster socially just and resilient

Belsky, Jill M.

419

NASA/ESA CV-990 Spacelab Simulation (ASSESS 2)  

NASA Technical Reports Server (NTRS)

To test the validity of the ARC approach to Spacelab, several missions simulating aspects of Spacelab operations have been conducted as part of the ASSESS Program. Each mission was designed to evaluate potential Shuttle/Spacelab concepts in increasing detail. For this mission, emphasis was placed on development and exercise of management techniques planned for Spacelab using management participants from NASA and ESA who have responsibilities for Spacelab 1 which will be launched in 1980.

Mulholland, D. R.; Androes, G. M.; Reeves, J. F.

1978-01-01

420

Katherine Yanhang Zhang's CV, page 1 Katherine Yanhang Zhang  

E-print Network

and collagen digested arterial tissue as a model of aortic aneurysm, in preparation. J-23. Zou, Y. and Zhang, Y., 2011, Structure and function of arterial elastin in solutes, in preparation. J-22. Xu, B. and Zhang, Y Articles in Preparation J-24. Chow, M-J, and Zhang, Y., 2011, Mechanical and structural changes in elastin

421

McGuire CV Page 1 of 9  

E-print Network

and interacts with a large geothermal power generation operation to produce abundant seismicity and aseismic-Doctoral Fellowship Professional Affiliations Member American Geophysical Union, Seismological Society of America

McGuire, Jeff

422

CV-November 2013 Page 1 of 3  

E-print Network

of Internal Combustion Engine Supervisor: Prof. Vahid Esfahanian University of Semnan- Semnan, IRAN BS, M. (2009). "Numerical 1-D Simulation of Internal Combustion Engines considering Entropy Level Changes," Proceeding of The sixth International Conference on Internal Combustion Engines, Tehran, Iran

Central Florida, University of

423

OpenCV and TYZX : video surveillance for tracking.  

SciTech Connect

As part of the National Security Engineering Institute (NSEI) project, several sensors were developed in conjunction with an assessment algorithm. A camera system was developed in-house to track the locations of personnel within a secure room. In addition, a commercial, off-the-shelf (COTS) tracking system developed by TYZX was examined. TYZX is a Bay Area start-up that has developed its own tracking hardware and software which we use as COTS support for robust tracking. This report discusses the pros and cons of each camera system, how they work, a proposed data fusion method, and some visual results. Distributed, embedded image processing solutions show the most promise in their ability to track multiple targets in complex environments and in real-time. Future work on the camera system may include three-dimensional volumetric tracking by using multiple simple cameras, Kalman or particle filtering, automated camera calibration and registration, and gesture or path recognition.

He, Jim; Spencer, Andrew; Chu, Eric

2008-08-01

424

Determination of some physical properties of date fruit (cv. Mazafati)  

Microsoft Academic Search

along length (PL), width (PW), and thickness (PT) were 462.80, 716.33 and 749.86 mm 2 , respectively. The fruit density and pitted fruit density were measured 0.82and 1.25g\\/cm3 while bulk density and porosity were 0.44g\\/cm3 and 44.90%, respectively. The geometric mean diameter, sphericity and surface area were obtained as 27.87mm, 0.73, and 2446.70mm 2 , respectively. The mean coefficients of

M. K. Jahromi

425

Hudman CV -1/6 Rynda C. Hudman  

E-print Network

air quality over the Western U.S. by developing regressions between area burned and meteorology driven, aircraft, and satellite air pollutant observations; statistical regressions of meteorological and land Computing: Fortran, IDL, Matlab, R, C, sh/Perl, Mathematica; Microsoft Office, Expressions Languages

Cohen, Ronald C.

426

Sarig 1 CV (2013) Curriculum Vita of Omri Moshe Sarig  

E-print Network

://www.wisdom.weizmann.ac.il/ sarigo EDUCATION Doctorate: Ph.D. (with distinction), awarded in 2001 Tel-Aviv University. Supervisor: J University, Pennsylvania, USA 2003­2006 Assistant Professor of Mathematics (tenure track) The Pennsylvania, Italy Markov partitions for surface diffeomorphisms (3 two­hour lectures) Poncelet Laboratory, Moscow

427

Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae  

E-print Network

at the forefront of their peers." 2004 Fellow, Center for Advanced Study in the Behavioral Sciences (CASBS) Summer: Affective Forecasting for Benevolent and Selfish Behaviors (co-PI, with PI Jeffrey Green), $90,000. 2008's Alumnae Association, Oxytocin, Speed-Dating, and Trustworthiness (PI), $1,000. 2011 Northwestern

Reber, Paul J.

428

Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae  

E-print Network

). 2004 Fellow, Center for Advanced Study in the Behavioral Sciences (CASBS) Summer Institute: Emotion as Learning From Life Experiences: Affective Forecasting for Benevolent and Selfish Behaviors (co-PI, with PI Gráinne Fitzsimons), $9,900. 2011 Northwestern's Alumnae Association, Oxytocin, Speed

Reber, Paul J.

429

Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae  

E-print Network

that places them at the forefront of their peers." 2004 Fellow, Center for Advanced Study in the Behavioral: Affective Forecasting for Benevolent and Selfish Behaviors (co-PI, with PI Jeffrey Green), $90,000. 2008 Northwestern's Alumnae Association, Oxytocin, Speed-Dating, and Trustworthiness (PI), $1,000. 2011 Northwestern

Reber, Paul J.

430

A New Magnetic CV: EUVE J2115-58.6  

Microsoft Academic Search

We report on a newly identified magnetic cataclysmic variable, EUVE J2115-58.6, observed as part of the EUVE Optical ID Campaign on 1995 December 10 with the 1.5 m telescope at CTIO\\\\@. An optical counterpart for EUVE J2115-58.6 has been identified and has the following position: R.A. = 21(h15^m41^s) , Decl. = -58d40arcmin 54arcsec , well within the source error circle.

M. M. Sirk; N. Craig; D. Christian; B. Roberts; S. Howell; P. McGee

1996-01-01

431

CV of Keivan Esfarjani Page 1 KEIVAN ESFARJANI  

E-print Network

Ecoles" 81 83 Lycee Pierre de Fermat, Toulouse, France. Research-related Keywords Methods: High of Delaware, Newark, DE, GPA 4.0/4.0 Dissertation: Study of quantum melting in 2D, application to Coulomb, France General-Engineering degree 83 86 Ecole Centrale de Paris, Chatenay Malabry, France "Maitrise

Glyde, Henry R.

432

CATHY J. BUSBY-ABBREVIATED CV Publications posted at  

E-print Network

and Structure, Geological Catastrophes, Tectonics of Sedimentary Basins, Sedimentology and Stratigraphy, Geology of Convergent Margins and Continental Rifts, Cordilleran Tectonics, Submarine Volcanism, Turbidite Sedimentology, Spain, 1998; A.A.P.G.-S.E.P.M. Annual Meeting, 1998; Second Latinamerican Sedimentological Congress

Busby, Cathy

433

CV: Leigh G. Torres Leigh G. Torres, Ph.D.  

E-print Network

and Wildlife Marine Mammal Institute, Oregon State University Hatfield Marine Science Center 2030 SE Marine through spatial analysis and genetics. Marine Mammal Science 19(3):502-514. Torres, L.G., W.A. McLellan, E of bottlenose dolphins (Tursiops truncatus) in Florida Bay, Florida. Marine Mammal Science 25(4): 797

Kurapov, Alexander

434

HARVARDgazette15-28 OCTOBER 2009 VOL. CV NO. 4  

E-print Network

Terry L. Murphy Managing Editor, Harvard Gazette Georgia Bellas Calendar Editor Corydon Ireland Staff.harvard.edu/gazette/story/ 2009/10/arts_medalist/ MUSIC DEPT. CHAIR THOMAS KELLY HONORED WITH CONFERENCE The Music Department with a confer- ence called "City, Chant, and the To- pography of Early Music." news.harvard.edu/gazette/story/2

435

Possible Supernova and new CV detected by MASTER  

NASA Astrophysics Data System (ADS)

MASTER-Kislovodsk auto-detection system discovered OT source at (RA, Dec) = 00h 15m 02.14s +41d 32m 35.2s on 2013-08-30.86205 UT. The OT unfiltered magnitude is 17.1m (limit 18.2m). The OT is seen in 2 images. There is no minor planet at this place. We have reference image without OT on 2012-11-15.76179 UT with unfiltered magnitude limit 20.5m.

Balanutsa, P.; Yecheistov, V.; Lipunov, V.; Denisenko, D.; Gorbovskoy, E.; Tiurina, N.; Kornilov, V.; Shatskiy, N.; Chazov, V.; Kuznetsov, A.; Vladimirov, V.; Parkhomenko, A.; Tlatov, A.; Dormidontov, D.; Senik, V.; Yurkov, V.; Sergienko, Y.; Varda, D.; Sinyakov, E.; Gabovich, A.; Ivanov, K.; Yazev, S.; Budnev, N.; Konstantinov, E.; Chuvalaev, O.; Poleshchuk, V.; Gress, O.; Frolova, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Podvorotny, P.; Shumkov, V.; Shurpakov, S.; Levato, H.; Saffe, C.; Mallamaci, C.; Lopez, C.; Podest, F.

2013-09-01

436

Gabrielle Katz, CV, Page 1 of 9 GABRIELLE L. KATZ  

E-print Network

for Sonoran desert riparian ecosystems, & Urban ecology of desert riparian ecosystems, Tucson, Arizona. #12 of biodiversity, Geographical hydrology, Senior capstone seminar. Graduate courses: Research themes and methods

437

Extraterrestrial and Terrestrial Fassaite Compared: Allende CV3  

E-print Network

's substitution, along the diopside (Di: CaMgSi2O6) - Ca-Tchermak (CaTs: CaAlAlSiO6) join. We report the crystal, by single crystal X-ray diffraction (XRD), as well as cation ordering by 29 Si and 27 Al Magic Angle of Allende fassaite show two distinct sets of well resolved peaks representing octahedral and tetrahedral

Grossman, Lawrence

438

McGuire CV Page 1 of 8  

E-print Network

deformation left behind after the shaking subsides (geodesy). The majority of my contributions have focused developed new analytical techniques in both seismology and geodesy including the ability to resolve the structure of the seismogenic zone, and seafloor geodesy deployments to understain the build up of strain

McGuire, Jeff

439

McGuire CV Page 1 of 1  

E-print Network

left behind after the shaking subsides (geodesy). The majority of my contributions have focused developed new analytical techniques in both seismology and geodesy including the ability to resolve the structure of the seismogenic zone, and seafloor geodesy deployments to understand the build up of strain

McGuire, Jeff

440

McGuire CV Page 1 of 5  

E-print Network

deformation left behind after the shaking subsides (geodesy). The majority of my contributions have focused developed new analytical techniques in both seismology and geodesy including the ability to resolve and seafloor geodesy equipment at the Quebrada, Discovery, and Gofar transform faults of the East Pacific Rise

McGuire, Jeff

441

Catherine Graham CV (March 2014) Page 1 Catherine Helen Graham  

E-print Network

of montane biodiversity: integrating evolutionary and ecological processes. Ecography. (79) Weinstein, B, phylogenetic and trait betadiversity in South American hummingbirds. American Naturalist. (78) Holt, B and modellers in functional biodiversity research? Basic and Applied Ecology, 14: 93-101. (72) Schymanski. S

Davidson, Ana

442

An antifungal defensin from Phaseolus vulgaris cv. 'Cloud Bean'.  

PubMed

An antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3kDa was purified from dried seeds of Phaseolus vulgaris 'Cloud Bean'. The isolation procedure entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography an Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Although the antifungal peptide was unadsorbed on DEAE-cellulose, it was adsorbed on both Affi-gel blue gel and SP-Sepharose. The antifungal peptide exerted antifungal activity against Mycosphaerella arachidicola with an IC(50) value of 1.8 ?M. It was also active against Fusarium oxysporum with an IC(50) value of 2.2 ?M. It had no inhibitory effect on HIV-1 reverse transcriptase when tested up to 100 ?M. Proliferation of L1210 mouse leukemia cells and MBL2 lymphoma cells was inhibited by the antifungal peptide with an IC(50) of 10 ?M and 40 ?M, respectively. PMID:20729048

Wu, Xiangli; Sun, Jian; Zhang, Guoqing; Wang, Hexiang; Ng, Tzi Bun

2011-01-15

443

Aroma extract dilution analysis of Cv. Marion (Rubus spp. hyb) and Cv. Evergreen (R. laciniatus L.) blackberries.  

PubMed

Cultivar Marion and Evergreen blackberry aromas were analyzed by aroma extract dilution analysis. Sixty-three aromas were identified (some tentatively) by mass spectrometry and gas chromatography-retention time; 48 were common to both cultivars, and 27 have not been previously reported in blackberry fruit. A comparison of cultivars shows that both have comparable compound types and numbers but with widely differing aroma impacts, as measured by flavor dilution (FD) factors. Ethyl 2-methylbutanoate, ethyl 2-methylpropanoate, hexanal, furanones (2,5-dimethyl-4-hydroxy-3-(2H)-furanone, 2-ethyl-4-hydroxy-5-methyl-3-(2H)-furanone, 4-hydroxy-5-methyl-3-(2H)-furanone, 4,5-dimethyl-3-hydroxy-2-(5H)-furanone, and 5-ethyl-3-hydroxy-4-methyl-2-(5H)-furanone), and sulfur compounds (thiophene, dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide, 2-methylthiophene, and methional) were prominent in Evergreen (FD 512-2048). Except for ethyl 2-methylpropanoate, these same compounds were also prominent in Marion, but the FD factors varied significantly (FD 8-256) from Evergreen. The aroma profile of blackberry is complex, as no single volatile was unanimously described as characteristically blackberry. PMID:12744680

Klesk, Keith; Qian, Michael

2003-05-21

444

FLUCTUACION ANUAL DE CARBOHIDRATOS EN AGUACATE (Persea americana Mill.) CV COLIN V-33 CARBOHYDRATE CYCLING IN AVOCADO (Persea americana Mill.) CV COLIN V-33  

Microsoft Academic Search

The aim of this work was to evaluate if there were yearly variations in carbohydrate content of leaves and inflorescences and to determine if it is limiting for retention of flowers and fruits of avocado 'Colín V-33'. General reducing and non-reducing sugars, starch and specific weight were determined for leaves (north and south sides of the canopy) every month, and

Ana María; Castillo González; María Teresa Colinas León; María Luisa Ortega; Angel Martínez Garza

445

VARIACION ANUAL DE BORO EN AGUACATE (Persea americana Mill.) CV. COLIN V-33 BORON CYCLING IN AVOCADO (Persea americana Mill.) CV. COLIN V-33  

Microsoft Academic Search

Boron content on leaves (monthly) and inflorescences (in autumn and winter) of avocado 'Colín V-33' was made with the purpose of determinate the variation during the year and if it is limiting for retention of flowers and fruit set. The content of boron on leaves didn't change during the year in relation to phenology, therefore it no limits the retention

Ana María; Castillo González; Martín Rubí Arriaga; Angel Martínez Garza

446

Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity  

Microsoft Academic Search

The impact of water deficit and salt stress on two important wine grape cultivars, Chardonnay and Cabernet Sauvignon, was investigated. Plants were exposed to increasing salinity and water deficit stress over a 16 d time period. Measurements of stem water potentials, and shoot and leaf lengths indicated that Chardonnay was more tolerant to these stresses than Cabernet Sauvignon. Shoot tips

Delphine Vincent; Ali Ergul; Marlene C. Bohlman; Elizabeth A. R. Tattersall; Richard L. Tillett; Matthew D. Wheatley; Rebekah Woolsey; David R. Quilici; Johann Joets; Karen Schlauch; David A. Schooley; John C. Cushman; Grant R. Cramer

2007-01-01

447

Pre symptomatic detection of wheat leaf rust in the susceptible cv Skalmeje and the resistant cv Esket by means of UV laser-induced fluorescence.  

PubMed

In modern agriculture there is a great demand for a rapid and objective screening method for stress resistance, because so far, the resistance of new cultivars is tested in time- and money consuming field experiments. Based on fluorescence ratios, and lifetime of fluorophores measured by fluorescence spectroscopy, we have postulated that an early discrimination of susceptible and resistant wheat cultivars to the leaf rust pathogen Puccinia triticina can be accomplished. As representative for leaf rust resistant and leaf rust susceptible wheat genotypes the cultivars Esket and Skalmeje, respectively, were chosen. Plants were grown under controlled environment conditions and inoculated with the leaf rust pathogen at the second-leaf-stage by single-droplet application. Fluorescence measurements were carried out from two to four days after inoculation (dai) by using a compact fibre-optic fluorescence spectrometer with nanosecond time-resolution. Experimental results indicated that UV laser-induced spectral characteristics as well as determination of fluorescence lifetime are suited to detect leaf rust two dai. For this purpose several ratios and wavelength can be considered. In general, the tested cultivars showed distinct responses to the pathogen development. In this context the ratio F451/F687 measured three dai and mean lifetimes at 500 nm and 530 nm are suited to differentiate the resistant Esket from the susceptible Skalmeje genotypes. PMID:21542468

Bürling, K; Hunsche, M; Noga, G

2010-01-01

448

Factors regulating the growth rate of Lolium perenne L. cv. ‘Grasslands Ruanui’ and L. multiflorum Lam. cv. ‘Grasslands Tama’ a Tetraploid  

Microsoft Academic Search

When seedlings and young plants of these ryegrass varieties are glasshouse-grown in pots with adequate nutrient and light, Tama maintains a 14–16% higher mean relative growth rate (shoot) than Ruanui. A number of developmental, physiological, and biochemical assessments have been made in an attempt to explain some of the reasons for this difference in growth rate. Some discussion on the

A. O. Taylor; J. A. Rowley; N. M. Jepsen

1971-01-01

449

Graduate Council Meeting Agenda 310 Jack K. Williams Administration Building  

E-print Network

Contemporary Civil Wars k. INTA 672 East Asian Security l. INTA 673 Chinese Domestic Politics in Transition m "Approved by" have been removed. d. Academic Calendar. Dr Cunningham is the GC representative on the committee that is looking at the academic calendar. There is a proposal to allow students to have

450

William Wisden CV Curriculum Vitae: William Wisden, FSB, FMedSci  

E-print Network

-present) Awards Fellow of the Academy of Medical Sciences (elected April 2014) Membership of Professional Organizations: British Neuroscience Association (since 1987; committee member in the mid-90s) Society for Neuroscience (since 1993) European Biological Rhythms Society (since 2013) Fellow of the Society of Biology

451

A sample return mission to a pristine NEO submitted to ESA CV 2015-2025  

NASA Astrophysics Data System (ADS)

ESA Cosmic Vision 2015-2025 aims at furthering Europe's achievements in space science, for the benefit of all mankind. ESA' multinational Space Science Advisory Committee prepared the final plan, which contains a selection of themes and priorities. In the theme concerning how the Solar System works, a Near-Earth Object (NEO) sample return mission is indicated among the priorities. Indeed, small bodies, as primitive leftover building blocks of the Solar System formation process, offer clues to the chemical mixture from which the planets formed some 4.6 billion years ago. The Near Earth Objects (NEOs) are representative of the population of asteroids and dead comets and are thought to be similar in many ways to the ancient planetesimal swarms that accreted to form the planets. NEOs are thus fundamentally interesting and highly accessible targets for scientific research and space missions. A sample return space mission to a pristine NEO has thus been proposed in partnership with the Japanese Space Agency JAXA, involving a large European community of scientists. The principal objectives are to obtained crucial information about 1) the properties of the building blocks of the terrestrial planets; 2) the major events (e.g. agglomeration, heating, ... .) which ruled the history of planetesimals; 3) the properties of primitive asteroids which may contain presolar material unknown in meteoritic samples; 4) the organics in primitive materials; 5) the initial conditions and evolution history of the solar nebula; and 6) on the potential origin of molecules necessary for life. This project appears clearly to have the potential to revolutionize our understanding of primitive materials. It involves a main spacescraft which will allow the determination of important physical properties of the target (shape, mass, crater distribution . . . ) and which will take samples by a touch-and-go procedure, a Lander for in-situ investigation of the sampling site, and sampling depending on technological development and resource allocations, a re-entry capsule, and scientific payloads. We will present the mission targets, scenarios and techniques that have been proposed.

Michel, P.; Barucci, A.

2007-08-01

452

IEEE ELECTRON DEVICE LETTERS, VOL. 32, NO. 12, DECEMBER 2011 1659 Unusual CV Characteristics of  

E-print Network

, Inc., Samsung Electronics, Hynix, Siltronic, and SRC). The review of this letter was arranged Engineering, Arizona State University, Tempe, AZ 85287-5706 USA. M. Seacrist is with MEMC Electronic Materials

Schroder, Dieter K.

453

CV of Jaywan Chung Born: October 27th 1982, Republic of Korea.  

E-print Network

: Natural Science Research Institute, KAIST, 291 Daehak-ro(373-1 Guseong- dong), Yuseong-gu, Daejeon 305-701, Republic of Korea. e-maill: jaywan.chung@gmail.com Education · Ph.D. in Mathematical Sciences, KAIST, February 2011 · M.S. in Applied Mathematics, KAIST, February 2007 · B.S. in Mathematics, KAIST, February

Kim, Yong Jung

454

Economics of the Environment R. Quentin Grafton and John C.V. Pezzey  

E-print Network

, as far back as Adam Smith in the eighteenth century, some economists have taken a broad or holistic Economics as a separate discipline usually traces its roots back to the work of Adam Smith and some nations become so much wealthier than others? Investigating this led Smith to study individual

Pezzey, Jack

455

Leakage compensated charge method for determining static CV characteristics of ultra-thin MOS capacitors  

Microsoft Academic Search

We describe a charge measurement method to determine static capacitance versus voltage characteristics of ultra-thin silicon oxide dielectric (<~3.5 nm), metal-oxide-silicon device structures in which significant leakage current is present. The leakage is accounted for using current compensation circuitry in combination with elementary charge integration electronics. For the thinnest oxides where direct tunneling is significant (<~2.5 nm), the circuit is

Hui Song; Edwin Dons; Xi Qing Sun; K. R. Farmer

1998-01-01

456

Leakage compensated charge method for determining static CV characteristics of ultra-thin MOS capacitors  

Microsoft Academic Search

We describe a charge measurement method to determine static capacitance versus voltage characteristics of ultra-thin silicon oxide dielectric (

Hui Song; Edwin Dons; Xi Qing Sun; K. R. Farmer

1998-01-01

457

Page 1 of 8 Academic CV, MCWhitton Last revised 2012-01-02  

E-print Network

to Physical Therapy - Improving scenario/rehabilitation session generation tools for physical therapists - How ­ present Research Assistant Professor, May 1995 ­ July 2004 Manager of Virtual Environments Research

Whitton, Mary C.

458

Genetic transformation of date palm (Phoenix dactylifera L. cv. 'Estamaran') via particle bombardment.  

PubMed

In this study, an efficient transformation system for gene delivery in date palm was established. The effects of different physical and biological parameters were optimized for transient transformation of uidA gene in somatic embryos of Estamaran cultivar. The tissues were bombarded with constructs harboring the uidA gene driven by CaMV 35S or rice Act1 promoter. Efficiency of expression was estimated by comparison of the number of blue spots resulted from GUS assay. Optimal transient expression was observed when explants were precultured on a media containing 0.4 M mannitol with air desiccation and bombarded at acceleration pressure of 1,350 psi, target distance of 6 cm with gold particles size of 0.6 µm which coated with 2.5 µg of DNA and at chamber vacuum pressure of 28 inHg. Significantly higher expression levels were obtained in tissues when the construct having the Act1 promoter was employed. After bombardment, somatic embryos were transferred to the regeneration media containing MS basal salts supplements with 3 mg/l 2ip, 40 mg/l adenine, 1 mg/l 2,4-D, 30 g/l sucrose and 3 g/l activated charcoal. Regenerated plantlets were checked by PCR using gene-specific primers. About 16 % of the plantlets were reported to be stably transformed. Southern analysis of genomic DNA from transformed plants showed that 1-2 gene (uidA) copies were integrated and GUS-negative plants did not contain any transgene. Achievement of these data considered as the first report of its kind is believed to facilitate transfer of desirable traits in date palm. PMID:25200434

Mousavi, Mousa; Mousavi, Amir; Habashi, Ali Akbar; Dehsara, Bahareh

2014-12-01

459

Microsoft Word - 10-Formatted Speaker Abstracts_rev_.mv.cv.doc  

Cancer.gov

4-40 Presentation Title: Marriage After Cancer: State of the Science Author: Sharon Manne, Ph.D., Fox Chase Cancer Center Description: The majority of literature on the impact of cancer on families conceptualizes spouses as caregivers to patients

460

New spectroscopic and photometric observations of CV J0644+3344  

NASA Astrophysics Data System (ADS)

We report photometric and simultaneous spectroscopic observations of the cataclysmic variable J0644+3344 and present calibrated Doppler tomography results of the object. We have calibrated the spectra for slit losses using simultaneous photometry. This has been used to construct reliable Doppler images in the Halpha , Hbeta and He II 4686 Å@ emission lines. We have also performed a radial velocity analysis using these emission lines to derive the primary semi-amplitude and used a cophasing method to determine the secondary K_2 best value. We have improved the ephemeris of the object based on the published and our new photometric eclipse timings to obtain HJD=254474.7927+0.26937446E. We derived a K_1=123.2±6.6 {km s}-1 and K_2=205.4±6.7. Assuming a i>76, the orbital parameters are M_1=0.91±0.04 M?, M_2=0.83±0.04 M? and a=2.10±0.03 R?. Based on the Doppler tomography we conclude that J0644+3344 is a nova-like SW Sex type system.

Hernández Santisteban, J. V.

461

State of Alaska v. Lubchenco, et al. Case No. 3:10-CV-00271-TMB  

E-print Network

the preliminary draft environmental impact statement (EIS) for North Pacific Fishery Management Council Case 3 Committee, and the Advisory Panel. In addition, the Council posted the preliminary draft EIS Lion Mitigation Committee reviewed the preliminary draft EIS and developed a recommended preliminary

462

State of Alaska v. Lubchenco, et al. Case No. 3:10-CV-00271-TMB  

E-print Network

Management Area Environmental Impact Statement (EIS) to the North Pacific Fishery Management Council (Council). NMFS revised the preliminary draft EIS to address the comments from the Council to the extent possible, and released a draft EIS for public review and comment. The Notice of Availability of the draft EIS

463

Pawel Swietojanski CV, January 2014, page 1 of 3 PAWEL SWIETOJANSKI  

E-print Network

Science at Higher State Vocational School in Tarnow, Poland · Very good academic record (4.49 out of 5 and Laboratories · Since October 2010: SHVS Tarnow, Teaching Assistant / Scientific Specialist · October 2007 - October 2010: SHVS Tarnow, Scientific Specialist TALKS · Unsupervised Cross-lingual Knowledge Transfer

Edinburgh, University of

464

Proteomic analysis of salt stress responses in foxtail millet ( Setaria italica L. cv. Prasad) seedlings  

Microsoft Academic Search

Soil salinity is one of the major abiotic stresses limiting crop productivity and the geographical distribution of many important crops worldwide. To gain a better understanding of the salinity stress responses at molecular level in dry land small millet crop, foxtail millet (Setaria italica L.), we carried out a comparative proteomic analysis. Foxtail millet seeds were surface sterilized and germinated

G. Veeranagamallaiah; G. Jyothsnakumari; M. Thippeswamy; P. Chandra Obul Reddy; G.-K. Surabhi; G. Sriranganayakulu; Y. Mahesh; B. Rajasekhar; Ch. Madhurarekha; Chinta Sudhakar

2008-01-01

465

Microsoft Word - 10-Formatted Speaker Abstracts_rev_.mv.cv.doc  

Cancer.gov

4-12 Presentation Title: Physical Activity Following Cancer Treatment Author: Anna Schwartz, F.N.P., Ph.D., FAAN, Arizona State University Description: Cancer and its treatment are associated with numerous acute and long-term physical and

466

Blanck CV, Page 1 of 38 email at pblanck@syr.edu 14 May 2012  

E-print Network

of Labor/Veterans (DOL/VETS), Homeless Veterans Reintegration Project Technical Assistance Center, Gary. and Marion Kierscht Professor of Law, University of Iowa, College of Law 2000-2006 Director, Law, Health of Public Health, Department of Occupational Medicine and Environmental Health, Professor (by Courtesy) 1997

Mather, Patrick T.

467

A summary of the UV, optical and IR properties of disks in CV's. [cataclysmic variables  

NASA Technical Reports Server (NTRS)

The available observations of dwarf novae and novalike systems that are relevant to the study of accretion in cataclysmic variables are reviewed. This includes UV data from IUE and optical spectroscopy and infrared photometry. The flux distributions are compared to available steady state models to obtain an estimate of the mass accretion and the range of this parameter among the dwarf novae (U Gem, Z Cam and SU UMa types) and the novalikes (UX UMa, AM Her, DQ Her types). The normal and abnormal range of variation in the continuum and line fluxes is discussed.

Szkody, P.

1985-01-01

468

QTLs associated with resistance in soybean PI567516C to synthetic nematode population infecting cv. Hartwig  

Technology Transfer Automated Retrieval System (TEKTRAN)

Worldwide, soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most destructive pathogen of soybean [Glycine max (L.) Merr.]. Crop losses are primarily mitigated by the use of resistant cultivars. Nematode populations are variable and have adapted to reproduce on resistant cultivars ov...

469

Short CV as of August 2012 Dr. Karl-Friedrich Ziegahn  

E-print Network

such as sustainable economy, environmental compatibility and cleaner production strategies, waste recovery, closed experiences includes product design and qualification, reliability and service life, airbag technology serves as president of the German Society for Environmental Engineering (Gesellschaft für

Schmitt, Peter H.

470

An Energy-Efficient, Fast FPGA Hardware Architecture for OpenCV-Compatible Object  

E-print Network

Brousseau, Jonathan Rose Electrical and Computer Engineering, University of Toronto 10 Kings Collage Road of cameras and powerful computers on modern mobile devices gives rise to the hope that they can perform computer vision tasks as we walk around. However, the computational demand and energy consumption

Rose, Jonathan

471

Alian Wang: CV (December 2013) Curriculum Vita of Prof. Alian Wang  

E-print Network

MatISSE (Maturation of Instruments for Solar System Exploration) project for developing CIRS (Compact, Chinese Academy of Geological Sciences, Ministry of Land and Resources, China. Leading and Participating Instrument Development Program) project. 2007 ­ 2018, PI, a NASA Mars Scout-MoO (Mission of Opportunity

472

Detached white-dwarf close-binary stars – CV's extended family  

Microsoft Academic Search

I review detached binaries consisting of white dwarfs with either other white dwarfs or low mass main-sequence stars in tight orbits around them. Orbital periods have been measured for 15 white dwarf\\/white dwarf systems and 22 white dwarf\\/M dwarf systems. While small compared to the number of periods known for CVs (>300), I argue that each variety of detached system

T. R. Marsh

2000-01-01

473

Detached white-dwarf close-binary stars -- CV's extended family  

E-print Network

I review detached binaries consisting of white dwarfs with either other white dwarfs or low mass main-sequence stars in tight orbits around them. Orbital periods have been measured for 15 white dwarf/white dwarf systems and 22 white dwarf/M dwarf systems. While small compared to the number of periods known for CVs (>300), I argue that each variety of detached system has a space density an order of magnitude higher that of CVs. While theory matches the observed distribution of orbital periods of the white dwarf/white dwarf binaries, it predicts white dwarfs of much lower mass than observed. Amongst both types of binary are clear examples of helium core white dwarfs, as opposed to the usual CO composition; similar systems must exist amongst the CVs. White dwarf/M dwarf binaries suffer from selection effects which diminish the numbers seen at long and short periods. They are useful for the study of irradiation; I discuss evidence to suggest that Balmer emission is broadened by optical depth effects to an extent which limits its usefulness for imaging the secondary stars in CVs.

T. R. Marsh

1999-06-07

474

Perturbation of polyamine catabolism affects grape ripening of Vitis vinifera cv. Trincadeira.  

PubMed

Grapes are economically the most important fruit worldwide. However, the complexity of biological events that lead to ripening of nonclimacteric fruits is not fully understood, particularly the role of polyamines' catabolism. The transcriptional and metabolic profilings complemented with biochemical data were studied during ripening of Trincadeira grapes submitted to guazatine treatment, a potent inhibitor of polyamine oxidase activity. The mRNA expression profiles of one time point (EL 38) corresponding to harvest stage was compared between mock and guazatine treatments using Affymetrix GrapeGen(®) genome array. A total of 2113 probesets (1880 unigenes) were differentially expressed between these samples. Quantitative RT-PCR validated microarrays results being carried out for EL 35 (véraison berries), EL 36 (ripe berries) and EL 38 (harvest stage berries). Metabolic profiling using HPLC and (1)H NMR spectroscopy showed increase of putrescine, proline, threonine and 1-O-ethyl-?-glucoside in guazatine treated samples. Genes involved in amino acid, carbohydrate and water transport were down-regulated in guazatine treated samples suggesting that the strong dehydrated phenotype obtained in guazatine treated samples may be due to impaired transport mechanisms. Genes involved in terpenes' metabolism were differentially expressed between guazatine and mock treated samples. Altogether, results support an important role of polyamine catabolism in grape ripening namely in cell expansion and aroma development. PMID:24296250

Agudelo-Romero, Patricia; Ali, Kashif; Choi, Young H; Sousa, Lisete; Verpoorte, Rob; Tiburcio, Antonio F; Fortes, Ana M

2014-01-01

475

Purification and partial biochemical characterization of polyphenol oxidase from mango (Mangifera indica cv. Manila).  

PubMed

Polyphenol oxidase (PPO) is an enzyme widely distributed in the plant kingdom that has been detected in most fruits and vegetables. PPO was extracted and purified from Manila mango (Mangifera indica), and its biochemical properties were studied. PPO was purified 216-fold by hydrophobic interaction and ion exchange chromatography. PPO was purified to homogeneity, and the estimated PPO molecular weight (MW) by SDS-PAGE was ?31.5 kDa. However, a MW of 65 kDa was determined by gel filtration, indicating a dimeric structure for the native PPO. The isolated PPO showed the highest affinity to pyrogallol (Km = 2.77 mM) followed by 4-methylcatechol (Km = 3.14 mM) and catechol (Km = 15.14 mM). The optimum pH for activity was 6.0. PPO was stable in the temperature range of 20-70 °C. PPO activity was completely inhibited by tropolone, ascorbic acid, sodium metabisulfite, and kojic acid at 0.1 mM. PMID:25211397

Palma-Orozco, Gisela; Marrufo-Hernández, Norma A; Sampedro, José G; Nájera, Hugo

2014-10-01

476

Matthew P. Hare C.V. Page 1 MATTHEW P. HARE  

E-print Network

solidissima similis) found north of its reported range: A commercially harvested population in Long Island Sound, New York. Journal of Shellfish Research 29(4):799-807. 6. Zhang, H., J. Scarpa, and M.P. Hare

Keinan, Alon

477

Traffic-related air pollution biomonitoring with Tradescantia pallida (Rose) Hunt. cv. purpurea Boom in Brazil.  

PubMed

This study aimed to verify the capacity of Tradescantia pallida in the biomonitoring of air pollution in urban areas with different traffic intensities and under varying environmental conditions. Experiments were carried out in Ribeirão Preto, in the Southeastern Brazil, with more than 660,000 inhabitants and a fleet of more than 485,000 motor vehicles. Ten seedlings of T. pallida were exposed in three areas in the city, differing in traffic vehicle flow, in two seasons (wet and dry). At the end of each sampling period, which lasted 4 months, samples of leaves were collected, and the content of As, Ba, Ca, Cd, Cr, Cu, Fe, Hg, Mg, Mn, P, Pb, S, and Zn was determined by inductively coupled plasma mass spectroscopy (ICP-MS). The same elements were determined in soil samples for a seasonal characterization in conjunction with secondary data of environmental parameters. Additionally, micronucleus assay with early pollen tetrad cells of Tradescantia (Trad-MN) was conducted by collecting flower buds and analyzing the micronuclei frequencies in pollen mother cells. Although pollutant levels in air were below the Brazilian legal limits, plants exposed in the high-traffic flow area presented higher concentrations of elements related to vehicle emissions, especially under dry conditions, and higher micronuclei frequency in pollen mother cells. These results show the sensitivity of T. pallida to low-level urban air pollution and its suitability as bioindicator for trace elements. This alternative tool for biomonitoring can serve as a support methodology for the adoption of more restrictive public environmental policies in Brazil and extendible to other developing countries. PMID:25632896

Santos, Ana Paula M; Segura-Muñoz, Susana I; Nadal, Martí; Schuhmacher, Marta; Domingo, José L; Martinez, Carlos Alberto; Magosso Takayanagui, Angela M

2015-02-01

478

Genetic Dissection of Resistance in Soybean PI567516C to a Nematode Population Infecting cv. Hartwig  

Technology Transfer Automated Retrieval System (TEKTRAN)

Worldwide, soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most destructive pathogen of soybean [Glycine max (L.) Merr.]. Crop losses are primarily mitigated by the use of resistant cultivars. Nematode populations are variable and have adapted to reproduce on resistant cultivars ov...

479

Inheritance of Resistance in Soybean PI 567516C to LY1 Nematode Population Infecting cv. Hartwig  

Technology Transfer Automated Retrieval System (TEKTRAN)

Worldwide, cyst nematode (SCN: Heterodera glycines Ichinohe) is the most destructive pathogen on soybean [Glycine max (L.) Merr.]. In the USA yield losses in 2001 were estimated to be nearly sixty million dollars. Crop losses are primarily reduced by the use of resistant cultivars. Nematode populat...

480

Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation  

Microsoft Academic Search

BACKGROUND: Ultraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV-B treatment. Some reports have analyzed the changes in gene expression caused by UV-B light on several plant species using microarray technology.

Mariela A Pontin; Patricia N Piccoli; Rita Francisco; Ruben Bottini; Jose M Martinez-Zapater; Diego Lijavetzky

2010-01-01