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  1. Differential and scanning-mode external PIXE for the analysis of the painting “Ritratto Trivulzio” by Antonello da Messina

    NASA Astrophysics Data System (ADS)

    Grassi, Novella

    2009-03-01

    Extensive PIXE investigations have been performed at the LABEC accelerator laboratory in Florence on the painting "Ritratto Trivulzio" by Antonello da Messina, one of the great Italian masters of XV Century and a pioneer in modern oil painting. It is well known that a complete and unambiguous characterisation of materials in paintings is often difficult, owing to their complex structure. For the "Ritratto Trivulzio", the combination of advanced variants of PIXE, such as differential and scanning-mode analysis, provided a relevant contribution to the characterisation of paint layers, in terms of composition and structure, in a totally non-invasive and non-destructive way. Single-spot mode PIXE with external proton beams of about half millimetre diameter was first used for a general characterisation of different areas of the painting. Differential PIXE performed in many spots led to reconstruct the sequence of paints employed by the artist and to estimate in some cases the local paint layer thickness. A second run carried out with the external scanning microbeam facility, using a 3 MeV proton beam of about 80 μm size, was then crucial to clarify some issues raised by the first analysis. In particular, elemental maps from selected areas helped to understand the way some colour shades had been obtained on the red mantle of the portrayed gentleman, using different pigments irregularly distributed on the surface.

  2. Geodetic surveys in Messina straits area

    NASA Astrophysics Data System (ADS)

    Baldi, P.; Achilli, V.; Mulargia, F.; Broccio, F.

    1983-03-01

    By using the fit of theoretical displacements on a fault according to Mindlin and Cheng (1950) dislocation theory, the vertical deformation field (maximum subsidence of 70 cm) associated to the Dec. 28 1908 Messina M=7.0 earthquake is compared to the results of a spirit levelling survey that we performed in the Messina Straits area in 1981 1982. The differences in level from the last levelling campaign in the area, completed in 1970, show that the coastlines have undergone a moderate differential subsidence of about 1 mm/year in the last decade. This is in agreement with the trend observed around 1908 and with the data of the Messina tide-gauge relative to the period 1897 1908 and 1910 1918. The lack of data in the period 1918 1970 does not allow definite conclusions about the vertical tectonic deformations in the area. Recent data on planimetric deformations have shown a South-North relative motion of Sicily. A comparison with the theoretical displacements indicates that a pure dip-slip of 4 cm on the 1908 fault system does explain the observed vertical deformation, but not the horizontal.

  3. Tsunami risk assessments in Messina, Sicily - Italy

    NASA Astrophysics Data System (ADS)

    Grezio, A.; Gasparini, P.; Marzocchi, W.; Patera, A.; Tinti, S.

    2012-01-01

    We present a first detailed tsunami risk assessment for the city of Messina where one of the most destructive tsunami inundations of the last centuries occurred in 1908. In the tsunami hazard evaluation, probabilities are calculated through a new general modular Bayesian tool for Probability Tsunami Hazard Assessment. The estimation of losses of persons and buildings takes into account data collected directly or supplied by: (i) the Italian National Institute of Statistics that provides information on the population, on buildings and on many relevant social aspects; (ii) the Italian National Territory Agency that provides updated economic values of the buildings on the basis of their typology (residential, commercial, industrial) and location (streets); and (iii) the Train and Port Authorities. For human beings, a factor of time exposition is introduced and calculated in terms of hours per day in different places (private and public) and in terms of seasons, considering that some factors like the number of tourists can vary by one order of magnitude from January to August. Since the tsunami risk is a function of the run-up levels along the coast, a variable tsunami risk zone is defined as the area along the Messina coast where tsunami inundations may occur.

  4. Radiation processing with the Messina electron linac

    NASA Astrophysics Data System (ADS)

    Auditore, L.; Barnà, R. C.; De Pasquale, D.; Emanuele, U.; Loria, D.; Morgana, E.; Trifirò, A.; Trimarchi, M.

    2008-05-01

    In the last decades radiation processing has been more and more applied in several fields of industrial treatments and scientific research as a safe, reliable and economic technique. In order to improve existing industrial techniques and to develop new applications of this technology, at the Physics Department of Messina University a high power 5 MeV electron linac has been studied and set-up. The main features of the accelerating structure will be described together with the distinctive features of the delivered beam and several results obtained by electron beam irradiations, such as improvement of the characteristics of polymers and polymer composite materials, synthesis of new hydrogels for pharmaceutical and biomedical applications, reclaim of culture ground, sterilization of medical devices, development of new dosimeters for very high doses and dose rates required for monitoring of industrial irradiations.

  5. Stochastic seismic analysis in the Messina strait area

    SciTech Connect

    Cacciola, P.; Maugeri, N.; Muscolino, G.

    2008-07-08

    After 1908 Messina earthquake significant progresses have been carried out in the field of earthquake engineering. Usually seismic action is represented via the so called elastic response spectrum or alternatively by time histories of ground motion acceleration. Due the random nature of the seismic action, alternative representations assume the seismic action as zero-mean Gaussian process fully defined by the so-called Power Spectral Density function. Aim of this paper is the comparative study of the response of linear behaving structures adopting the above representation of the seismic action using recorded earthquakes in the Messina strait area. In this regard, a handy method for determining the power spectral density function of recorded earthquakes is proposed. Numerical examples conducted on the existing space truss located in Torre Faro (Messina) will show the effectiveness of stochastic approach for coping with the seismic analysis of structures.

  6. Bacterial pollution of Messina coastal waters: a one year study.

    PubMed

    Caruso, G; Zaccone, R; Monticelli, L; Crisafi, E; Zampino, D

    2000-07-01

    A year's monitoring of faecal pollution of marine coastal waters surrounding Messina was carried out in 1996/97. The distribution of faecal coliforms was evaluated in 15 stations located along the Sicilian coastline, sampled monthly in coincidence of the two opposing current phases ("montante" and "scendente" currents) which characterise the Straits of Messina. The data obtained provided a complete picture of hygienic-sanitary conditions of the area and highlighted the presence of heavily polluted sites in correspondence with river outflows. Higher bacterial counts were associated with lower salinity values and higher ammonia concentrations; over an annual study, they occurred during the coldest months, showing the negative impact of continental water inputs on the bacteriological quality of coastal waters. PMID:10939045

  7. Earthquake supersite project in the Messina Straits area (EQUAMES)

    NASA Astrophysics Data System (ADS)

    Mattia, Mario; Chiarabba, Claudio; Dell'Acqua, Fabio; Faccenna, Claudio; Lanari, Riccardo; Matteuzzi, Francesco; Neri, Giancarlo; Patanè, Domenico; Polonia, Alina; Prati, Claudio; Tinti, Stefano; Zerbini, Susanna

    2015-04-01

    A new permanent supersite is going to be proposed to the GEO GSNL (Geohazard Supersites and National Laboratories) for the Messina Straits area (Italy). The justification for this new supersite can be found in its geological and geophysical features and in the exposure to strong earthquakes, also in the recent past (1908). The Messina Supersite infrastructure (EQUAMES: EarthQUAkes in the MEssina Straits) will host, and contribute to the collection of, large amounts of data, basic for the analysis of seismic hazard/risk in this high seismic risk area, including risk from earthquake-related processes such as submarine mass failures and tsunamis. In EQUAMES, data of different types will coexist with models and methods useful for their analysis/interpretation and with first-level products of analysis that can be of interest for different kinds of users. EQUAMES will help all the interested scientific and non-scientific subjects to find and use data and to increase inter-institutional cooperation by addressing the following main topics in the Messina Straits area: • investigation of the geological and physical processes leading to the earthquake preparation and generation; • analysis of seismic shaking at ground (expected and observed); • combination of seismic hazard with vulnerability and exposure data for risk estimates; • analysis of tsunami generation, propagation and coastal inundation deriving from earthquake occurrence also through landslides due to instability conditions of subaerial and submarine slopes; • overall risk associated to earthquake activity in the Supersite area including the different types of cascade effects Many Italian and international Institutions have shown an effective interest in this project where a large variety of geophysical and geological in-situ data will be collected and where the INGV has the leading role with its large infrastructure of seismic, GPS and geochemical permanent stations. The groups supporting EQUAMES

  8. Geodetic surveys across the Messina Straits (southern Italy) seismogenetic area

    NASA Astrophysics Data System (ADS)

    Anzidei, M.; Baldi, P.; Bonini, C.; Casula, G.; Gandolfi, S.; Riguzzi, F.

    1998-01-01

    The Messina Straits, southern Italy, unfortunately became famous after the occurrence of the great earthquake of December 28, 1908, Ms = 7.5, that caused thousands of deaths and severe destruction over a wide area along the Sicilian and Calabrian coasts. After that time many geophysical and geological studies were performed to evaluate the seismic characteristics of the 1908 earthquake, the seismic risk and the geological evolution of this region in the framework of Mediterranean geodynamics. In 1970, a geodetic network was set up across the Straits and was repeatedly measured with terrestrial techniques until 1980, showing a northward displacement of the Sicilian sites with respect to the Calabrian ones, between 1970 and 1971. In 1987, the old terrestrial network was surveyed again for the first time by the GPS technique, improving the accuracy of the coordinate determinations. Finally, in 1994, a wider network was established and surveyed again to collect additional GPS observations from a larger area across the Straits. In this paper, an analysis of the results obtained from the two GPS surveys with respect to those achieved by the terrestrial surveys (from 1970 to 1980) is given. This analysis shows there has not been significant crustal horizontal deformation across the Straits in the last 15 years. Although this crustal tectonic 'quiescence' corresponds to a low seismic activity level in the Straits area, terrestrial and GPS geodetic results would agree with Straits geophysical models excluding any aseismic deformations acting perpendicular to the Straits axis.

  9. Special Fluid Viscous Dampers For The Messina Strait Bridge

    SciTech Connect

    Colato, Gian Paolo; Infanti, Samuele; Castellano, Maria Gabriella

    2008-07-08

    The Messina Strait Bridge would be the world's longest suspension bridge, with a design earthquake characterised by a PGA value of 0.58 g and a distance between the ipocenter and the bridge of 15 km. Said critical structure of course would need a suitable restraint system for traffic braking loads, wind and seismic actions. Each type of load requires a specific behaviour of the restraint system, making its design a big challenge.The restraint system comprises special types of fluid viscous dampers, installed both in longitudinal and transverse direction, both at the towers and at the anchorages. In seismic conditions they behave as viscous dampers, to reduce the forces on the structural elements and the movements of the bridge deck. But in service dynamic conditions, e.g. under traffic or wind load, the devices shall behave like shock transmission units, thus preventing the longitudinal and transverse movements of the deck.FIP Industriale cooperated with the selected General Contractor, a consortium lead by Impregilo, in the design of said viscous dampers. This paper describes the main features of said devices.

  10. New insights on the Messina 1908 seismic source from post-seismic sea level change

    NASA Astrophysics Data System (ADS)

    Cannelli, V.; Melini, D.; Piersanti, A.

    2013-08-01

    The identification of a source model for the catastrophic 1908 December 28 Messina earthquake (Mw = 7.2) has been the subject of many papers in the last decades. Several authors proposed different models on the basis of seismological, macroseismic and geodetic data sets; among these models, remarkable differences exist with regard to almost all parameters. We selected a subset of six models among those most cited in literature and used them to model the post-seismic sea level variation recorded at the tide gauge station of Messina (until 1923), to attempt an independent discrimination among them. For each model, we assumed a simple rheological structure and carried out a direct-search inversion of upper crust thickness and lower crust viscosity to fit the post-seismic sea level signal. This approach enabled us to identify a class of fault geometries which is consistent with the post-seismic signal at the Messina tide gauge and with the known structural and rheological features of the Messina strait.

  11. The tectonic puzzle of the Messina area (Southern Italy): insights from new seismic reflection data.

    PubMed

    Doglioni, Carlo; Ligi, Marco; Scrocca, Davide; Bigi, Sabina; Bortoluzzi, Giovanni; Carminati, Eugenio; Cuffaro, Marco; D'Oriano, Filippo; Forleo, Vittoria; Muccini, Filippo; Riguzzi, Federica

    2012-01-01

    The Messina Strait, that separates peninsular Italy from Sicily, is one of the most seismically active areas of the Mediterranean. The structure and seismotectonic setting of the region are poorly understood, although the area is highly populated and important infrastructures are planned there. New seismic reflection data have identified a number of faults, as well as a crustal scale NE-trending anticline few km north of the strait. These features are interpreted as due to active right-lateral transpression along the north-eastern Sicilian offshore, coexisting with extensional and right-lateral transtensional tectonics in the southern Messina Strait. This complex tectonic network appears to be controlled by independent and overlapping tectonic settings, due to the presence of a diffuse transfer zone between the SE-ward retreating Calabria subduction zone relative to slab advance in the western Sicilian side. PMID:23240075

  12. The tectonic puzzle of the Messina area (Southern Italy): Insights from new seismic reflection data

    PubMed Central

    Doglioni, Carlo; Ligi, Marco; Scrocca, Davide; Bigi, Sabina; Bortoluzzi, Giovanni; Carminati, Eugenio; Cuffaro, Marco; D'Oriano, Filippo; Forleo, Vittoria; Muccini, Filippo; Riguzzi, Federica

    2012-01-01

    The Messina Strait, that separates peninsular Italy from Sicily, is one of the most seismically active areas of the Mediterranean. The structure and seismotectonic setting of the region are poorly understood, although the area is highly populated and important infrastructures are planned there. New seismic reflection data have identified a number of faults, as well as a crustal scale NE-trending anticline few km north of the strait. These features are interpreted as due to active right-lateral transpression along the north-eastern Sicilian offshore, coexisting with extensional and right-lateral transtensional tectonics in the southern Messina Strait. This complex tectonic network appears to be controlled by independent and overlapping tectonic settings, due to the presence of a diffuse transfer zone between the SE-ward retreating Calabria subduction zone relative to slab advance in the western Sicilian side. PMID:23240075

  13. The Scientific Contribution by Arturo Danusso after the 1908 Messina-Reggio Calabria Earthquake

    SciTech Connect

    Sorrentino, Luigi

    2008-07-08

    After 1908 Messina and Reggio Calabria earthquake the Italian scientific community produced a considerable effort, also as a consequence of two competitions. In both contests a Piedmont engineer, Arturo Danusso, prevailed. In this paper emphasis will be put on the suggested building layout and construction details, which are far ahead of their time. Moreover, the dynamic analysis of a linear elastic undamped single- and two-degree-of freedom system, proposed by Danusso and elsewhere already discussed, will be briefly mentioned.

  14. Archaeometallurgy in Messina: Iron Slug From A Dig at Blog P, Laboratory Analyses and Interpretation.

    NASA Astrophysics Data System (ADS)

    Caterina, Ingoglia; Maurizio, Triscari; Giuseppe, Sabatino

    The archaeological site in Via La Farina, Block P, in Messina, is unique in many ways, due also to the high quantity of samples of iron slag. The slag was examined to identify the production centres of such materials, and, after characterization, was compared to similar material, exclusively for product typology, from different archaeological sites in the province of Messina, situated in the Peloritani Mountains (Messina city, S. Marco d'Alunzio, Milazzo, Francavilla di Sicilia, Novara di Sicilia as well as the archaeological site of Halaesa, near Tusa). Mineralogical characterization of the phases carried out by X-ray diffractometry (XRD) and Rietveld data elaboration, morphological study of slag findings and a semi-quantitative analysis by scanning electronic microscope (SEM+EDX) were performed. A chemical investigation was carried out by electron probe micro analysis (EPMA), to determine major element,. Minor and trace elements were determined by LA-ICP-MS. All the examined slag is related to iron metallurgy, and, in the case of Via La Farina, there is firm archaeological evidence pinpointing to smelting activity.

  15. Linking onshore and offshore erosion and sediment transport in the Strait of Messina, Italy

    NASA Astrophysics Data System (ADS)

    Goswami, R.; Mitchell, N. C.; Brocklehurst, S. H.; Argnani, A.

    2010-12-01

    The Sicilian and Calabrian coasts either side of the Strait of Messina in southern Italy provide an opportunity to observe the response of subaerial drainage systems to variations in bedrock erodibility and uplift rate. Offshore processes include hyperpycnal currents derived from onshore drainages, as well as longitudinal flow along the Messina Channel. The narrow continental shelves allow a direct connection between these onshore and offshore processes. NE Sicily and SW Calabria are mainly composed of granite, high-grade gneiss, limestone and meta-pelite. Published Late Pleistocene to Holocene uplift rates from bedrock terraces and erosional notches indicate that uplife rates increase away from the Strait, on both sides. Digital topographic analyses reveal that, on the Sicilian side, longitudinal channel profiles exhibit generally concave upward trends with many locally steep reaches (knickpoints). Many of the large scale knickpoints (150-200 m in height) occur at drainage areas below the transition from debris-flow dominated colluvial channels to stream-flow dominated fluvial channels. There is a positive but weak correlation between channel steepness and uplift rate. This correlation is strongest near the drainage divide, with a weaker correlation downstream, where a major shear zone generally reduces the bedrock resistance, irrespective of lithology. The distribution of landslides has been mapped on both sides of the Strait of Messina using aerial photography, revealing contrasting controls on landslide distribution. In Sicily, landslides are most frequent where uplift rates are most rapid and rainfall heaviest. The Calabrian landscape is less incised, so landslides are confined to valley sides, with low-relief interfluves free of landslides. Offshore, geomorphic features have been characterised from multi-beam bathymetric and Chirp data. The central feature is the Messina Channel, which runs north-south. Although the channel does not meander prominently

  16. The 1908 Messina Tsunami: the most likely sources from marine data

    NASA Astrophysics Data System (ADS)

    Tappin, D. R.; Watts, P.

    2010-12-01

    Dangerous, if not devastating, tsunamis can be sourced from submarine mass failures. Research over the past decade has resulted in an improved recognition of this hazard. Numerous anomalous tsunamis may have a submarine landslide component, and these are identified by runups that, to some degree, are too large in relation to their proposed earthquake source. They include Sanriku, 1896, Messina, 1908, Puerto Rico 1918, Indian Ocean, 1945, Alaska, 1946 and Flores Island, 1982. Re-evaluation of these events, most notably using improved sea bed mapping technologies, has improved our understanding of their source, but not always with a proven result. Thus we are still challenged in our ability to differentiate historical tsunamis that may be sourced from either earthquakes or submarine landslides. Qualification of tsunami sources is critical if we are to validate submarine landslides as an important hazard. The 1908 Messina tsunami is one such enigmatic event, but (for its time) unique in that there is a remarkable catalogue of tsunami inundation and runup data that provides a control on the actual tsunami source(s). The centenary of the 1908 Messina earthquake and tsunami has come and gone, yet over 100 years after this event took place, no complete model of the tsunami source mechanism(s) has yet been produced. Previous analyses of the 1908 tsunami have looked at a wide variety of earthquake rupture mechanisms, and several possible mass failure events. Whereas progress has been made in understanding the event, there still remains some doubt as to the validity of the geological interpretations and, therefore, subsequent tsunami modeling results. Using marine geology data, notably multibeam bathymetry, we present a new unified model of the tsunami and its sources, providing a complete simulation of the event (Watts and Tappin, 2010). The earthquake rupture mechanism is by faulting along stepovers in the Messina Strait. Individual fault segments have NNW-SSE trending

  17. The surface effects of the 1908 Southern Calabria - Messina earthquake (Southern italy)

    NASA Astrophysics Data System (ADS)

    Comerci, V.; Blumetti, A. M.; Brustia, E.; di Manna, P.; Fiorenza, D.; Guerrieri, L.; Lucarini, M.; Serva, L.; Vittori, E.

    2009-04-01

    The December 28, 1908, Southern Calabria - Messina earthquake (Intensity MCS XI, Mw 7.24; Stucchi et al, 2007) was the strongest seismic event of the 20th century in Italy and the most ruinous in terms of casualties (at least 80,000). According to Michelini et al. (2005) its epicentre was located at sea in the southern part of the Messina Straits. The damages were particularly catastrophic along the Calabrian coast, between south of Reggio Calabria and south-west of Scilla, and along the eastern coast of Sicily from its easternmost tip to south of Messina (Baratta, 1910; Boschi et al., 1995; Barbano et al., 2005). Messina and Reggio Calabria were almost totally destroyed. Few minutes after the earthquake, both sides of the Straits were inundated by several tsunami waves, worsening the ruinous effects due directly to the earthquake. We have collected and catalogued all the described coseismic effects on the environment by means of a careful screening of contemporary documents, i.e. technical and photographic reports, newspapers and other archive material. Inside 447 different testimonies of environmental effects, we have identified 290 independent occurrences. Among these effects, particularly relevant were the changes in elevation (mainly ground lowering) along both sides of the Straits, partly due to the settlement of loose sediments and artificial filling (e.g., Messina and Reggio Calabria port areas), and partly ascribed to landslides and tectonic deformation. Liquefaction phenomena, described as water, mud or sand ejection, occurred in the areas of Messina, lake Pantano (Ganzirri) and Reggio Calabria. Portions of the coast were lost, especially on the Calabrian side, most of them because of landslides and the combined action of the tsunami erosion and the earthquake shaking (at Lazzàro the shoreline retreated landward 175 m; Novarese, 1909). Ground cracks were reported in 69 localities, most of them in Messina, Reggio Calabria and Villa San Giovanni. 80 slope

  18. Geodynamics and seismic hazard in the Calabrian Arc: towards a Messina earthquake supersite

    NASA Astrophysics Data System (ADS)

    Chiarabba, Claudio; Dell'Acqua, Fabio; Faccenna, Claudio; Lanari, Riccardo; Matteuzzi, Francesco; Mattia, Mario; Neri, Giancarlo; Patané, Domenico; Polonia, Alina; Prati, Claudio; Tinti, Stefano; Zerbini, Susanna; Ozener, Haluk

    2015-04-01

    The Messina region represents a key site of the Mediterranean, where active faulting, seismic shaking, volcanism, rapid uplift and landslides represent the surface manifestation of deep processes. Fast deformation results in one of the highest seismic hazard of the Mediterranean, as testified by historic destructive earthquakes occasionally accompanied by submarine mass flows and tsunami-events that added death and destruction to the already devastating effects of the earthquakes. Several geophysical and geological studies carried out during the last decades help defining the kinematics and the dynamics of the system. The tectonic evolution of the Messina region is strictly linked with the Southern Tyrrhenian and Calabrian Arc system, the retreat of the Ionian slab and the back-arc basin opening. The present-day geometry of the Calabrian slab, as well imaged by tomographic analyses and shallow-to-deep seismicity, shows a narrow slab plunging down steeply into the mantle. At 100-150 km depth, the southern edge of the slab is positioned beneath Northeastern Sicily, approximately between Tindari and Messina. Within this frame, several relevant questions are still unsolved. For example, it is not clear how the upper plate may deform as a response of a differential sinking of the subducting slabs, or how deep mantle flow at the slab edge may influence the pattern of surface deformation. Structural and geodetic data show the first-order pattern of deformation in Northeastern Sicily, and define the Tindari-Messina area as the boundary between a region in compression to the west, dominated by the Africa convergence, and a region in extension to the east-northeast, dominated by slab rollback. In addition, geodetic studies also show an increase of crustal motion velocity from Sicily to Calabria with an overall clockwise rotation of the velocity vector. This pattern of surface deformation evidences a sharp extension process active in the Messina region. The elevation of

  19. The Calabria - Messina earthquake of December 28, 1908: remember to reduce the risk.

    NASA Astrophysics Data System (ADS)

    Nostro, C.; Tertulliani, A.; Pignone, M.; Pino, N. A.; de Gori, P.; Castellano, C.; Arcoraci, L.; Camassi, R.

    2009-04-01

    At 5:20 a.m., on 28th December 1908, one of the most catastrophic earthquakes in history occurred in the Messina Straits. Southern Calabria and north-eastern Sicily were severely hit: in few seconds Messina, Reggio Calabria and numerous villages on both sides of the Straits were devastated. Shortly after the earthquake a tsunami followed. Waves up to 12 meters high hit the Straits and the entire eastern Sicily coast. This is the strongest earthquake (magnitude 7.1) occurred in Italy in the twentieth century. The picture of the total damage was catastrophic: the estimated number of deaths is of around 80,000. In Messina the victims were about 60,000, corresponding to 40% of the population at the time, while in Reggio Calabria 12,000 were killed out of 45,000. To mark the centenary of this disaster, we arranged a program of different initiatives: 1) a photographic calendar with many information about the town destroyed and the tsunami effects (www.ingv.it); 2) a special workshop for general public to reconstruct what happened 100 years ago, to understand what we know today about earthquake and seismic risk, and how we can reduce the damage caused by earthquake; 3) many EDURISK training courses for more than 500 teachers of nursery, primary and lower secondary schools in Calabria and Sicily. These courses are based on a multidisciplinary approach and the educational experiences had been fitted to the specific local environment of each school, from the geographical, historical and cultural point of view (www.edurisk.it). 4) participation in ERMES 2008's activities to improve public awareness on seismic risks. The 1908 earthquake Group and the Edurisk team: E. Baroux, R. Azzaro, G. Ricciardi, M. Crescimbene, F. La Longa,V. Pessina.

  20. Tidal forcing of the water mass interface in the strait of Messina

    SciTech Connect

    Hopkins, T.S.; Salusti, E.; Settimi, D.

    1984-03-20

    The tidal currents of the Strait of Messina and their effect on the displacement of the water-mass interface are discussed utilizing the historical velocity data of Francesco Vercelli, taken from approx.100 anchor stations during 1922 and 1923. The data show a complex, strongly tidal behavior, in which speeds of 200-300 cm/s are attained over the sill. Messina is an amphidromic point for the tides of the two main basins of the Mediterranean Sea and two distinct water masses flow through the strait at different stages of the tide; the subsurface Laventine intermediate water flowing alternating with surface Tyrrhenian water flowing south. The time evolution of the interface between the two marine water layers at the semidiurnal periodicity is computed from the observed distribution of acceleration term for the baroclinic component. This interface shows large vertical oscillations with amplitudes of approx.100 m intersecting both the surface and bottom. The results are in reasonable agreement with recent satellite data and other recent field observations and numerical experiments. An estimate of the mean position of the interface is made by assuming that the potential energy required to uplift the interface, from the equilibrium depth of 150 m to the observed 30 m depth over the sill, is derived from the time-independent contribution of the nonlinear field acceleration term. The results are in reasonable agreement with observations.

  1. The surface effects of the 1908 Southern Calabria - Messina earthquake (Southern italy)

    NASA Astrophysics Data System (ADS)

    Comerci, V.; Blumetti, A. M.; Brustia, E.; di Manna, P.; Fiorenza, D.; Guerrieri, L.; Lucarini, M.; Serva, L.; Vittori, E.

    2009-04-01

    The December 28, 1908, Southern Calabria - Messina earthquake (Intensity MCS XI, Mw 7.24; Stucchi et al, 2007) was the strongest seismic event of the 20th century in Italy and the most ruinous in terms of casualties (at least 80,000). According to Michelini et al. (2005) its epicentre was located at sea in the southern part of the Messina Straits. The damages were particularly catastrophic along the Calabrian coast, between south of Reggio Calabria and south-west of Scilla, and along the eastern coast of Sicily from its easternmost tip to south of Messina (Baratta, 1910; Boschi et al., 1995; Barbano et al., 2005). Messina and Reggio Calabria were almost totally destroyed. Few minutes after the earthquake, both sides of the Straits were inundated by several tsunami waves, worsening the ruinous effects due directly to the earthquake. We have collected and catalogued all the described coseismic effects on the environment by means of a careful screening of contemporary documents, i.e. technical and photographic reports, newspapers and other archive material. Inside 447 different testimonies of environmental effects, we have identified 290 independent occurrences. Among these effects, particularly relevant were the changes in elevation (mainly ground lowering) along both sides of the Straits, partly due to the settlement of loose sediments and artificial filling (e.g., Messina and Reggio Calabria port areas), and partly ascribed to landslides and tectonic deformation. Liquefaction phenomena, described as water, mud or sand ejection, occurred in the areas of Messina, lake Pantano (Ganzirri) and Reggio Calabria. Portions of the coast were lost, especially on the Calabrian side, most of them because of landslides and the combined action of the tsunami erosion and the earthquake shaking (at Lazzàro the shoreline retreated landward 175 m; Novarese, 1909). Ground cracks were reported in 69 localities, most of them in Messina, Reggio Calabria and Villa San Giovanni. 80 slope

  2. Non Destructive Investigation on the 17th/18th Century Sicilian Jewellery Collection at the Messina Regional Museum Using Mobile Raman Equipment

    NASA Astrophysics Data System (ADS)

    Barone, G.; Bersani, D.; Jehlicka, J.; Lottici, P. P.; Mazzoleni, P.; Raneri, S.; Vandenabeele, P.; Di Giacomo, C.; Larinà, G.

    2014-06-01

    A handheld Raman spectrometer operating at 785 nm was used for the in situ analysis of the gems present in the 17th/18th century Sicilian jewelry collection preserved in the Messina Regional Museum (Italy).

  3. Broadband waveform inversion of moderate earthquakes in the Messina Straits, southern Italy

    NASA Astrophysics Data System (ADS)

    D'Amico, Sebastiano; Orecchio, Barbara; Presti, Debora; Zhu, Lupei; Herrmann, Robert B.; Neri, Giancarlo

    2010-04-01

    We report the first application of the Cut and Paste (CAP) method to compute earthquake focal mechanisms in the Messina Straits area by waveform inversion of Pnl and surface wave segments. This application of CAP has furnished new knowledge about low-magnitude earthquake mechanics that will be useful for improved understanding of the local geodynamics. This is possible because the CAP inversion technique can be applied to small earthquakes, for which traditional moment tensor inversion methods are not appropriate and P-onset focal mechanisms in the study area fail because of a lack of sufficient observations. We estimate the focal mechanisms of 23 earthquakes with local magnitudes in the range of 3-4 occurring in the 2004-2008 time period, and recorded by the broadband stations of the Italian National Seismic Network and the Mediterranean Very Broadband Seismographic Network (MedNet) run by the Istituto Nazionale di Geofisica e Vulcanologia (INGV). The solutions show that normal faulting is the prevailing style of seismic deformation in the northern part of the study area while co-existence of normal faulting and strike-slip has been detected in the southern part. In the whole area of investigation the T-axes of focal mechanisms display a preferential northwest-southeast direction of extension. Combined with the findings of previous investigations, this improved database of focal mechanisms allows us to better detail the transitional area between the extensional domain related to subduction trench retreat (southern Calabria) and the compressional one associated with continental collision (western-central Sicily). The observed spatial change of seismic deformation regime offers new data to current seismotectonic and seismic hazard investigations in the area of Messina Straits where a magnitude 7.2 earthquake caused more than 60,000 casualties on 28 December 1908.

  4. Basis of Design and Seismic Action for Long Suspension Bridges: the case of the Messina Strait Bridge

    SciTech Connect

    Bontempi, Franco

    2008-07-08

    The basis of design for complex structures like suspension bridges is reviewed. Specific attention is devoted to seismic action and to the performance required and to the connected structural analysis. Uncertainty is specially addressed by probabilistic and soft-computing techniques. The paper makes punctual reference to the work end the experience developed during the last years for the re-design of the Messina Strait Bridge.

  5. Effect of plasmolysis on protocorm-like bodies of Dendrobium Bobby Messina orchid following cryopreservation with encapsulation-dehydration method.

    PubMed

    Antony, Jessica Jeyanthi James; Mubbarakh, Safiah Ahmad; Mahmood, Maziah; Subramaniam, Sreeramanan

    2014-02-01

    Histological observation and scanning electron microscopy analyses in Dendrobium Bobby Messina indicates the cellular process of cryopreserved protocorm-like bodies (PLBs) was different comparative to non-cryopreserved PLBs. The cellular process was not only modified by the freezing and thawing effect but also due to the dehydration process itself during the cryopreservation procedure. Histological observation in Dendrobium Bobby Messina in encapsulation-dehydration method indicated that the degree of plasmolysis causes more cellular changes to the cryopreserved PLBs comparative to non-cryopreserved and stock culture PLBs. These results revealed higher amount of homogenous cell population and denser cytoplasm in cryopreserved PLBs. Histological analysis also revealed more voluminous nucleus in cryopreserved PLBs comparative to non-cryopreserved PLBs and PLBs stock culture. In contrast, scanning electron microscope analysis showed severe damages in cryopreserved PLBs and non-cryopreserved PLBs comparative to the PLBs stock culture which in return could be the possible reason of no regrowth in encapsulation-dehydration method. Damages incurred were on top part, side part, and at the stomata of the PLBs. Histological observation and scanning electron microscopy analyses in Dendrobium Bobby Messina indicates that the degree of plasmolysis causes changes in the cellular process of PLBs from cryopreserved PLBs was different comparative to non-cryopreserved PLBs. PMID:24218184

  6. Hydrodynamic modelling of coastal seas: the role of tidal dynamics in the Messina Strait, Western Mediterranean Sea

    NASA Astrophysics Data System (ADS)

    Cucco, Andrea; Quattrocchi, Giovanni; Olita, Antonio; Fazioli, Leopoldo; Ribotti, Alberto; Sinerchia, Matteo; Tedesco, Costanza; Sorgente, Roberto

    2016-07-01

    This work explores the importance of considering tidal dynamics when modelling the general circulation in the Messina Strait, a narrow passage connecting the Tyrrhenian and the Ionian subbasins in the Western Mediterranean Sea. The tides and the induced water circulation in this Strait are among the most intense oceanographic processes in the Mediterranean Sea. The quantification of these effects can be particularly relevant for operational oceanographic systems aimed to provide short-term predictions of the main hydrodynamics in the Western Mediterranean subbasins. A numerical approach based on the use of a high-resolution hydrodynamic model was followed to reproduce the tides propagation and the wind-induced and thermohaline water circulation within the Strait and in surrounding areas. A set of numerical simulations was carried out to quantify the role of the Strait dynamics on the larger-scale water circulation. The obtained results confirmed the importance of a correct representation of the hydrodynamics in the Messina Strait even when focusing on predicting the water circulation in the external sea traits. In fact, model results show that tidal dynamics deeply impact the reproduction of the instantaneous and residual circulation pattern, waters thermohaline properties and transport dynamics both inside the Messina Strait and in the surrounding coastal and open waters.

  7. VizieR Online Data Catalog: RACE-OC project. II. (Messina+, 2011)

    NASA Astrophysics Data System (ADS)

    Messina, S.; Desidera, S.; Lanzafame, A. C.; Turatto, M.; Guinan, E. F.

    2011-10-01

    Rotational properties of late-type low-mass members of associations of known age provide a fundamental source of information on stellar internal structure and its evolution. We aim at determining the rotational and magnetic-related activity properties of stars at different stages of evolution. We focus our attention primarily on members of young stellar associations of known ages. Specifically, we extend our previous analysis in Paper I (Messina et al., 2010, Cat. J/A+A/520/A15) to 3 additional young stellar associations beyond 100pc and with ages in the range 6-40Myr: {epsilon} Chamaeleontis (~6Myr), Octans (~20Myr), and Argus (~40Myr). Additional rotational data of {eta} Chamaeleontis and IC 2391 clusters are also considered. Rotational periods were determined by applying the Lomb-Scargle periodogram technique to photometric time-series data obtained by the All Sky Automated Survey (ASAS) and the Wide Angle Search for Planets (SuperWASP) archives. The magnetic activity level was derived from the amplitude of the V light curves. (4 data files).

  8. The metabolic profile of lemon juice by proton HR-MAS NMR: the case of the PGI Interdonato Lemon of Messina.

    PubMed

    Cicero, Nicola; Corsaro, Carmelo; Salvo, Andrea; Vasi, Sebastiano; Giofré, Salvatore V; Ferrantelli, Vincenzo; Di Stefano, Vita; Mallamace, Domenico; Dugo, Giacomo

    2015-01-01

    We have studied by means of High Resolution Magic Angle Spinning Nuclear Magnetic Resonance (HR-MAS NMR) the metabolic profile of the famous Sicilian lemon known as 'Interdonato Lemon of Messina PGI'. The PGI Interdonato Lemon of Messina possesses high organoleptic and healthy properties and is recognised as one of the most nutrient fruits. In particular, some of its constituents are actively studied for their chemo-preventive and therapeutic properties. In this paper, we have determined by means of HR-MAS NMR spectroscopy the molar concentration of the main metabolites constituent the juice of PGI Interdonato Lemon of Messina in comparison with that of the not-PGI Interdonato Lemon of Turkey. Our aim is to develop an analytical technique, in order to determine a metabolic fingerprint able to reveal commercial frauds in national and international markets. PMID:25702767

  9. Remote sensing as tool for development of landslide databases: The case of the Messina Province (Italy) geodatabase

    NASA Astrophysics Data System (ADS)

    Ciampalini, Andrea; Raspini, Federico; Bianchini, Silvia; Frodella, William; Bardi, Federica; Lagomarsino, Daniela; Di Traglia, Federico; Moretti, Sandro; Proietti, Chiara; Pagliara, Paola; Onori, Roberta; Corazza, Angelo; Duro, Andrea; Basile, Giuseppe; Casagli, Nicola

    2015-11-01

    Landslide geodatabases, including inventories and thematic data, today are fundamental tools for national and/or local authorities in susceptibility, hazard and risk management. A well organized landslide geo-database contains different kinds of data such as past information (landslide inventory maps), ancillary data and updated remote sensing (space-borne and ground based) data, which can be integrated in order to produce landslide susceptibility maps, updated landslide inventory maps and hazard and risk assessment maps. Italy is strongly affected by landslide phenomena which cause victims and significant economic damage to buildings and infrastructure, loss of productive soils and pasture lands. In particular, the Messina Province (southern Italy) represents an area where landslides are recurrent and characterized by high magnitude, due to several predisposing factors (e.g. morphology, land use, lithologies) and different triggering mechanisms (meteorological conditions, seismicity, active tectonics and volcanic activity). For this area, a geodatabase was created by using different monitoring techniques, including remote sensing (e.g. SAR satellite ERS1/2, ENVISAT, RADARSAT-1, TerraSAR-X, COSMO-SkyMed) data, and in situ measurements (e.g. GBInSAR, damage assessment). In this paper a complete landslide geodatabase of the Messina Province, designed following the requirements of the local and national Civil Protection authorities, is presented. This geo-database was used to produce maps (e.g. susceptibility, ground deformation velocities, damage assessment, risk zonation) which today are constantly used by the Civil Protection authorities to manage the landslide hazard of the Messina Province.

  10. The 1st October 2009 Messina debris flows: first analysis for a susceptibility model

    NASA Astrophysics Data System (ADS)

    Agnesi, Valerio; Cappadonia, Chiara; Conoscenti, Christian; Costanzo, Dario; Pino, Paolo; Puglisi, Claudio; Rotigliano, Edoardo

    2010-05-01

    In the evening of the 1st of October 2009, a sector of the Messina district (Sicily, Italy) was struck by a number of debris flows, triggered by extraordinary intense rainfall that, from 2 pm to 10 pm, discharged an amount of more than 160 mm and that followed the ones of September 23-24 (more than 200 mm in 10 hours). A number of villages (Altolia, Briga, Giampilieri, Guidomandri, Itala, Molino, Pezzolo, Scaletta), suffered for severe damages, including the destruction of houses and small buildings and more of 30 deaths. The area is located South from the city of Messina and mainly includes five short fluvial basins, that from the Peloritanian chain drain south-eastward for some kilometres to the Ionian sea. The area is characterized by the outcropping of metamorphic rocks and, due to the closeness of the chain (ranging up to 1200 meters a.s.l.) to the sea, the steepness of the slopes is typically very high. The debris flows involved the shallow layer made up of colluvial/eluvial and landslide deposits, having a thickness of some decimetres; both pure debris flow and debris slide movements have been inferred at the initiation zones, in light of the morphologic features of the source area (scarps). Also, according to the specific patterns recognized for the flow track zone, four typologies have been distinguished: ribbon-shaped, triangular, arch-shaped and multi-lobed debris flow. The landslides moved fast, as single or multiple/successive confluent style, so that already at the medium sector of the slopes, where the villages are, huge volumes of the debris flowed. Due to the shallowness of the failure zone, the high water content and velocity, the tracks of the debris flows have been highly controlled by hydrography, reaching, where no obstacles were present, the valley floor, with kilometric run-out distances. To each of the 379 recognized debris flows, which produced a total landslide area of about 7 km2, a landslide identification point (LIP) has been assigned

  11. Aeromagnetic investigation of southern Calabria and the Messina Straits (Italy): Tracking seismogenic sources of 1783 and 1908 earthquakes

    NASA Astrophysics Data System (ADS)

    Minelli, Liliana; Vecchio, Antonio; Speranza, Fabio; Nicolosi, Iacopo; D'Ajello Caracciolo, Francesca; Chiappini, Stefano; Carluccio, Roberto; Chiappini, Massimo

    2016-03-01

    Southern Calabria and the NE corner of Sicily (Italy) were struck in 1783 and 1908 A.D. by two of the most catastrophic earthquakes ever in European history. Although it is generally acknowledged that the seisms were yielded by normal faults rupturing the upper crust of the Calabria-Peloritani terrane, no consensus exists on seismogenic source location and orientation. Here we report on a high-resolution low-altitude aeromagnetic survey of southern Calabria and Messina Straits. In southern Calabria we document a broad weakly positive (5-10 nT) anomaly zone interrupted by three en echelon SW-NE null to negative magnetic anomaly corridors. Euler deconvolution and magnetic modeling show that the anomaly pattern is produced by a 1-1.5 km thick crustal "layer" located within 3 km depth. This layer is offset by a 25 km long NE trending fault that corresponds to the Armo normal fault, recently inferred to be the source for the 1908 earthquake. Few kilometers to the south, we also document a subparallel and previously unrecognized fault, entering the Messina Straits and likely joining the Armo fault at depth. Further east, we model a 40 km long normal fault, probably extending northeastward for additional 40 km, running along the south Calabria axis from Aspromonte to the Serre mountains and partly following the 18 km long surface rupture witnessed by Déodat de Dolomieu after the 1783 earthquake. Thus, aeromagnetic data suggest that the sources of the 1783 and 1908 earthquakes are en echelon faults belonging to the same NW dipping normal fault system straddling the whole southern Calabria.

  12. Determination and quantification of PCBs, POCs and PAHs in Thunnus thynnus from the Straits of Messina (Italy)

    PubMed Central

    Saija, Emanuele; Mangano, Valentina; Casale, Katia Ermina; La Torre, Giovanna Loredana; Dugo, Giacomo; Salvo, Andrea

    2016-01-01

    This data set is composed to assess the accumulation of persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs) dioxin like (DL) and not dioxin like (NDL), organochlorine pesticides (POCs) and polycyclic aromatic hydrocarbons (PAHs) in Thunnus thynnus and to elucidate the suitability of this species as a bioindicator for monitoring contaminations of these compounds in the marine ecosystems of the Straits of Messina. This investigation was conducted on liver samples of 14 T. thynnus collected during April 2015. Quantitative determination of PCBs (DL and NDL), POCs and PAHs in the examined samples has been carried out by HRGC-MS/MS. Among the PCBs, high prevalence of DL was found while, generally, the values detected for PCBs–NDL were lower than the legal limits. Tuna samples analyzed for PAHs residues revealed that all the samples were contaminated with acenaphthalene, fluorene, phenanthrene and anthracene. Moreover, generally the residual levels of DDT and DDT metabolites were low. The total content of PCB–DL, in almost all the samples, showed higher concentration than the legal limit. PMID:26933670

  13. Feeding habits of the Atlantic bluefin tuna, Thunnus thynnus (L. 1758), in the central Mediterranean Sea (Strait of Messina)

    NASA Astrophysics Data System (ADS)

    Battaglia, Pietro; Andaloro, Franco; Consoli, Pierpaolo; Esposito, Valentina; Malara, Danilo; Musolino, Simona; Pedà, Cristina; Romeo, Teresa

    2013-03-01

    The study of feeding habits of the Atlantic bluefin tuna was carried out in 123 specimens, ranging from 115 to 222 cm fork length (FL) and collected during spring seasons of 2010 and 2011 in the central Mediterranean Sea (Strait of Messina). The analysis of stomach contents allowed us to identify 91 taxa of prey items, mainly belonging to Teleostea (54), Cephalopoda (20) and Crustacea (13). The percentage of index of relative abundance ( IRI) shows the highest values for the myctophid Hygophum benoiti (% IRI = 22.854) and the stomiid Chauliodus sloani (% IRI = 15.124), followed by the oegopsid squid Illex coindetii (% IRI = 14.316). The broad spectrum of prey items could suggest a generalist behavior of this predator, with several species that occasionally occurs in its diet. However, if prey are grouped into food categories, the importance of mesopelagic and benthopelagic fishes can be appreciated (54.41 % of % IRI). The assessment of the hypothetical foraging rhythm of the Atlantic bluefin tuna highlighted that its feeding activity is concentrated on diel migrating fauna during night and on larger preys upon daylight. The predation on the high-energetic food as mesopelagic and bathypelagic fishes during the pre-spawning and the spawning period may bring an energetic advantage in tuna metabolism and gonadal maturation

  14. Determination and quantification of PCBs, POCs and PAHs in Thunnus thynnus from the Straits of Messina (Italy).

    PubMed

    Saija, Emanuele; Mangano, Valentina; Casale, Katia Ermina; La Torre, Giovanna Loredana; Dugo, Giacomo; Salvo, Andrea

    2016-06-01

    This data set is composed to assess the accumulation of persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs) dioxin like (DL) and not dioxin like (NDL), organochlorine pesticides (POCs) and polycyclic aromatic hydrocarbons (PAHs) in Thunnus thynnus and to elucidate the suitability of this species as a bioindicator for monitoring contaminations of these compounds in the marine ecosystems of the Straits of Messina. This investigation was conducted on liver samples of 14 T. thynnus collected during April 2015. Quantitative determination of PCBs (DL and NDL), POCs and PAHs in the examined samples has been carried out by HRGC-MS/MS. Among the PCBs, high prevalence of DL was found while, generally, the values detected for PCBs-NDL were lower than the legal limits. Tuna samples analyzed for PAHs residues revealed that all the samples were contaminated with acenaphthalene, fluorene, phenanthrene and anthracene. Moreover, generally the residual levels of DDT and DDT metabolites were low. The total content of PCB-DL, in almost all the samples, showed higher concentration than the legal limit. PMID:26933670

  15. The assessment of human health impact caused by industrial and civil activities in the Pace Valley of Messina.

    PubMed

    Morra, P; Lisi, R; Spadoni, G; Maschio, G

    2009-06-01

    The impact of industrial and civil activities on an agricultural and residential area is presented in a detailed and global analysis. The examined area is the Pace river valley situated in the northern zone of Messina (Italy). The sources of pollution present in the area are: a Municipal Solid Waste Incinerator operating since 1979, a disused urban solid waste landfill which was used for 30 years, an urban solid waste treatment facility with heavy vehicles traffic, and two open pits for the production of bitumen. Large quantities of toxic, carcinogenic substances and criteria pollutants are released into the environment and represent potential hazards to human health. The analysis is performed using the EHHRA-GIS tool which employs an integrated, multimedia, multi-exposure pathways and multi-receptor risk assessment model that is able to manage all the steps which constitute the human health risk analysis in a georeferenced structure. The transport of pollutants in different environmental media is assessed applying models (AERMOD, GMS, CALINE) that take into account the particular three-dimensional morphology of the terrain. The results obtained, combined with a probabilistic risk assessment and a sensitivity analysis of calculation parameters, are a comprehensive assessment of the total human health risk in the area. Finally human health risks caused by toxic and carcinogenic substances are compared with acceptable legal limits in order to support environmental managers' decisions. PMID:19344932

  16. Impact of Uncertainty on Loss Estimates for a Repeat of the 1908 Messina-Reggio Calabria Earthquake in Southern Italy

    SciTech Connect

    Franco, Guillermo; Shen-Tu, Bing Ming; Bazzurro, Paolo; Goretti, Agostino; Valensise, Gianluca

    2008-07-08

    Increasing sophistication in the insurance and reinsurance market is stimulating the move towards catastrophe models that offer a greater degree of flexibility in the definition of model parameters and model assumptions. This study explores the impact of uncertainty in the input parameters on the loss estimates by departing from the exclusive usage of mean values to establish the earthquake event mechanism, the ground motion fields, or the damageability of the building stock. Here the potential losses due to a repeat of the 1908 Messina-Reggio Calabria event are calculated using different plausible alternatives found in the literature that encompass 12 event scenarios, 2 different ground motion prediction equations, and 16 combinations of damage functions for the building stock, a total of 384 loss scenarios. These results constitute the basis for a sensitivity analysis of the different assumptions on the loss estimates that allows the model user to estimate the impact of the uncertainty on input parameters and the potential spread of the model results. For the event under scrutiny, average losses would amount today to about 9.000 to 10.000 million Euros. The uncertainty in the model parameters is reflected in the high coefficient of variation of this loss, reaching approximately 45%. The choice of ground motion prediction equations and vulnerability functions of the building stock contribute the most to the uncertainty in loss estimates. This indicates that the application of non-local-specific information has a great impact on the spread of potential catastrophic losses. In order to close this uncertainty gap, more exhaustive documentation practices in insurance portfolios will have to go hand in hand with greater flexibility in the model input parameters.

  17. Debris flow hazard assessment by integrated modeling of landslide triggering and propagation: application to the Messina Province, Italy

    NASA Astrophysics Data System (ADS)

    Stancanelli, L. M.; Peres, D. J.; Cavallaro, L.; Cancelliere, A.; Foti, E.

    2014-12-01

    During the last decades an increase of debris flow catastrophic events has been recorded along the Italian territory, mainly due to the increment of settlements and human activities in mountain areas. Considering the large extent of debris flow prone areas, non structural protection strategies should be preferably implemented because of economic constrains associated with structural mitigation measures. In such a framework hazard assessment methodologies play a key role representing useful tools for the development of emergency management policies. The aim of the present study is to apply an integrated debris flow hazard assessment methodology, where rainfall probabilistic analysis and physically-based landslide triggering and propagation models are combined. In particular, the probabilistic rainfall analysis provides the forcing scenarios of different return periods, which are then used as input to a model based on combination of the USGS TRIGRS and the FLO-2D codes. The TRIGRS model (Baum et al., 2008; 2010), developed for analyzing shallow landslide triggering is based on an analytical solution of linearized forms of the Richards' infiltration equation and an infinite-slope stability calculation to estimate the timing and locations of slope failures, while the FLO-2D (O'Brien 1986) is a two-dimensional finite difference model that simulates debris flow propagation following a mono-phase approach, based on empirical quadratic rheological relation developed by O'Brien and Julien (1985). Various aspects of the combination of the models are analyzed, giving a particular focus on the possible variations of triggered amounts compatible with a given return period. The methodology is applied to the case study area of the Messina Province in Italy, which has been recently struck by severe events, as the one of the 1st October 2009 which hit the Giampilieri Village causing 37 fatalities. Results are analyzed to assess the potential hazard that may affect the densely

  18. The priority of Antonino D'Antona in describing rhabdomyolysis with acute kidney injury, following the Messina earthquake (December 28, 1908). Commentary.

    PubMed

    De Santo, Natale Gaspare; Bisaccia, Carmela; De Santo, Luca Salvatore

    2016-01-01

    Following the Messina-Reggio Calabria earthquake (December 28, 1908) outstanding medical reports were published by Franz von Colmers (1875-1960), Antonino D'Antona (1842-1913), and Rocco Caminiti (1868-1940). The reports of D'Antona and Caminiti were heretofore neglected. Colmers, D'Antona and Caminiti described crush-syndrome. D'Antona who cured patients in shock also described two deaths due to uraemia. This gives him a priority in the description of crush syndrome with renal injury which has been traditionally attributed to Bywaters and Beall. PMID:27033609

  19. Prevalence of antibodies against Rickettsia conorii, Babesia canis, Ehrlichia canis, and Anaplasma phagocytophilum antigens in dogs from the Stretto di Messina area (Italy).

    PubMed

    Pennisi, Maria-Grazia; Caprì, Alessandra; Solano-Gallego, Laia; Lombardo, Gabriella; Torina, Alessandra; Masucci, Marisa

    2012-12-01

    The aims of this study were to determine the seroprevalence for Rickettsia conorii, Ehrlichia canis, Anaplasma phagocytophilum, and Babesia canis in outdoor-kennelled dogs (n=249) from the Stretto di Messina (Italy) and to compare seroprevalence in 2 public shelters and 4 privately-owned kennels where different tick-preventive measures were implemented in order to focus on the specific sanitary risk posed by public shelters in southern Italy for tick-borne pathogens. R. conorii (72%) and B. canis (70%) were the most prevalent infections when compared to E. canis (46%) and A. phagocytophilum (38%). Seroprevalence for R. conorii, E. canis, and A. phagocytophilum was significantly higher in public shelters than in private kennels. However, B. canis seropositivity was similar in both types of kennels. In addition, in private kennels where a regular ectocide treatment was carried out by means of spot-on devices, dogs did not present E. canis and A. phagocytophilum antibodies. One hundred fifty-one dogs out of 249 (61%) were seropositive to more than one pathogen with R. conorii and B. canis the most common ones. Coinfections were more frequently found in public-shelter dogs. This study demonstrated high seroprevalences against R. conorii, B. canis, E. canis, and A. phagocytophilum in kennelled dogs from both coastal sites of the Stretto di Messina and the importance of regular tick-bite prevention by means of individual spot-on devices. PMID:23140895

  20. Evaluation of debris flow susceptibility by means of a transferability procedure: a study case in Messina area

    NASA Astrophysics Data System (ADS)

    Cama, Mariaelena; Luigi, Lombardo; Conoscenti, Christian; Rotigliano, Edoardo

    2016-04-01

    Debris flows can be described as rapid mass movements, gravity induced able to transport large quantities of material downslope. This type of landslides is strongly controlled by the topography and usually occur in region characterized by steep slopes and at least seasonal heavy rainfall. One of the problem when dealing with debris flow susceptible areas is that the eroded surface is usually very shallow and it can be masked by the vegetation already few years after a landslide event. Therefore, debris flow prone areas very often suffer from lack of reliable landslide inventories necessary to calibrate and validate susceptibility models. In order to deal with this problem, transferability procedure (spatial partition) have already proved to be efficient in areas which show analogous topographic, lithological and climatic characteristics. A procedure to evaluate whether it is possible to apply model transferability is here proposed. This approach is based on the assumption that debris flow trigger in different locations under similar topographic conditions and includes: i) a test of similarity between training and test areas aimed at identifying thresholds in catchment similarity which allow to successfully perform the transferability; ii) the calibration of the susceptibility model in the training area; iii) the validation of the model on the test area. The debris flow susceptibility is here evaluated using a stochastic approach and the all procedure is implemented in a R script which can be easily used to test the procedure in other catchments. The study areas chosen to perform this study are located in the Messina province (southern Italy) respectively on the Ionian sector (Itala catchment) and on the Tyrrhenian sector (Saponara catchment). Itala catchment was hit by the sadly known debris flow event of the 1st October 2009 (37 fatalities and huge damages) while Saponara catchment on the 22nd November 2011 (only two years after the 2009 event) experienced a very

  1. Short-term Variations of the Zooplankton Community Near the Straits of Messina (North-eastern Sicily): Relationships with the Hydrodynamic Regime

    NASA Astrophysics Data System (ADS)

    Zagami, G.; Badalamenti, F.; Guglielmo, L.; Manganaro, A.

    1996-05-01

    Zooplankton samples were collected near the Straits of Messina over two 24-h periods in May 1988 and January 1989. In this area, the complex system of currents which circulate through the Straits still affects both biotic and hydrological factors even near the shore. Copepoda, Cladocera, Chaetognatha and Appendicularia together with some meroplanktonic taxa make up the zooplankton assemblage collected during the two periods. The community structure changes greatly in terms of biomass and density during the 24-h cycle, and in terms of species composition between the two sampling months. The variability of biomass and density could be ascribed to the effect of the special current system in the area, whereas variations in species composition could be related to the seasonality demonstrated by the zooplankton community. These factors combined are responsible for the peculiar condition described in the paper.

  2. Are the source models of the M 7.1 1908 Messina Straits earthquake reliable? Insights from a novel inversion and a sensitivity analysis of levelling data

    NASA Astrophysics Data System (ADS)

    Aloisi, M.; Bruno, V.; Cannavò, F.; Ferranti, L.; Mattia, M.; Monaco, C.; Palano, M.

    2013-03-01

    For decades, many authors have attempted to define the location, geometry and kinematics of the causative fault for the 1908 December 28, M 7.1 earthquake that struck the Messina Straits between Sicily and Calabria (southern Italy). The coseismic displacement caused a predominant downwarping of the Straits and small land uplift away from it, which were documented by levelling surveys performed 1 yr before and immediately after the earthquake. Most of the source models based on inversion of levelling data suggested that the earthquake was caused by a low angle, east-dipping blind normal fault, whose upper projection intersects the Earth surface on the Sicilian (west) side of the Messina Straits. An alternative interpretation holds that the causative fault is one of the high-angle, west-dipping faults located in southern Calabria, on the eastern side of the Straits, and may in large part coincide with the mapped Armo Fault. Here, we critically review the levelling data with the aim of defining both their usefulness and limits in modelling the seismogenic fault. We demonstrate that the levelling data alone are not capable of discriminating between the two oppositely dipping fault models, and thus their role as a keystone for modellers is untenable. However, new morphotectonic and geodetic data indicate that the Armo Fault has very recent activity and is accumulating strain. The surface observations, together with appraisal of macroseismic intensity distribution, available seismic tomography and marine geophysical evidence, lends credit to the hypothesis that the Armo and possibly the S. Eufemia faults are part of a major crustal structure that slipped during the 1908 earthquake.

  3. The role of Internal Solitary Waves on deep-water sedimentary processes: the case of up-slope migrating sediment waves off the Messina Strait

    NASA Astrophysics Data System (ADS)

    Droghei, Riccardo; Falcini, Federico; Martorelli, Eleonora; Casalbore, Daniele; Mosetti, Renzo; Salusti, Ettore; Sannino, Gianmaria; Santoleri, Rosalia; Chiocci, Francesco

    2016-04-01

    Joint marine geology and physical oceanography studies seek to demonstrate the inherited connection between seafloor sedimentary processes and seawater dynamics in a fruitful exchange. While seafloor morphology highlights the long-term action of bottom currents, oceanographic models attempt to explain and predict morphogenetic processes and sedimentary pattern at the seafloor [Blodeaux, 2001; Martorelli et al., 2010; Belde et al., 2015]. A sand waves field we observed off the Messina Strait (Mediterranean Sea) give us the opportunity to demonstrate the value of such a multidisciplinary approach. We interpret these sand-waves as formed by tidal-induced internal solitary waves (ISWs) that generate within the Strait [Alpers and Salusti, 1983; Sapia and Salusti; 1987; Artale et al., 1990; Bradt et al., 1999]. We hypothesize that the deflected pattern (i.e., the depth-dependent orientation) of these sand waves is due to refraction of ISWs occurring at the interface between the Levantine Intermediate Water (LIW) and the Modified Atlantic Water (MAW), caused by interaction with a topographic mound; while the motion of sediment is caused by the bottom velocity field associated with the ISW trough. Both numerical and in situ data provide hints regarding the formation of the observed geometries and give useful information about their dynamics and migration rate. We believe that our work represents an innovative and promising link between the geological and oceanographic communities, adding some insights on the role of ISWs on sedimentary process and the structure of continental margins [Puig et al, 2004; Haren et al., 2013]. References: Blondeaux, P. (2001). Mechanics of coastal forms. Annual Review of Fluid Mechanics, 33(1), 339-370. Martorelli, E., Falcini, F., Salusti, E., & Chiocci, F. L. (2010). Analysis and modeling of contourite drifts and contour currents off promontories in the Italian Seas (Mediterranean Sea). Marine Geology, 278(1), 19-30. Belde, J., Back, S

  4. Antonino D'Antona (1842-1913) was the first in describing the crush syndrome with renal failure following the Messina earthquake of December 28, 1908.

    PubMed

    Bisaccia, Carmela; De Santo, Natale Gaspare; De Santo, Luca S

    2016-02-01

    There is confusion about the first description of the association between crush syndrome and renal failure. It has been traditionally attributed to Bywaters and Beall. The present study aims to analyze the problem by analyzing medical reports on the Messina-Reggio Calabria earth-quake of December 28, 1908 by using documents heretofore unknown. It demonstrates that first description of rabdomyolysis with renal failure is attributed to Antonino DAntona (1842- 1913). DAntona, professor of surgery at the University of Naples, coordinated the health net organized in Naples to assist persons wounded during the quake. Many of them in shock were transferred to Naples by ships. Franz von Colmers (1875-1960) was the chief surgeon of the German Mission of the Red Cross after the quake. Because his late arrival, he did not treat patients with shock. He described rabdomyolysis. The third medical report is that of Rocco Caminiti (1868-1946), collaborator of DAntona at the University of Naples, and chief of surgery at the Loreto Hospital. He directed a rescue group in Villa San Giovanni and Reggio Calabria. In 1910, he reported on rabdomyolysis in patients treated in the place of the disaster. Therefore the present study indicates that Antonino DAntona holds the priority for description of rabdomyolysis and kidney injury. There is no longer a place for the eponym Bywaters syndrome. PMID:26913878

  5. Interannual variability, growth, reproduction and feeding of Pelagia noctiluca (Cnidaria: Scyphozoa) in the Straits of Messina (Central Mediterranean Sea): Linkages with temperature and diet

    NASA Astrophysics Data System (ADS)

    Rosa, S.; Pansera, M.; Granata, A.; Guglielmo, L.

    2013-02-01

    To identify some of the possible environmental factors stimulating the increasingly frequent outbreaks of the scyphomedusa Pelagia noctiluca in the Straits of Messina, we investigated its abundance, growth, reproduction and feeding over a 4-year period, from 2007 to 2011, at two coastal sites. Using either field investigations and manipulative experiments we show that, among the various factors considered, shifts in water temperature (influencing medusae metabolism, growth and reproduction rates) and the size structure of the zooplankton community (their natural preys) can promote the proliferation of P. noctiluca. In particular, we show that increased temperature let jellyfishes to grow more rapidly and reach exceptional sizes. We also report a peculiar opportunistic behavior of P. noctiluca, which makes this species a potentially strong competitor in the pelagic trophic web of the Straits ecosystem. We therefore propose that more frequent P. noctiluca outbreaks stimulated by increasing sea surface temperature and shifts in their prey availability and composition would become, in the near future, a major cause of ecosystem shift.

  6. Mapping rainfall-induced landslides and inundated areas using remote sensing technology and field surveys: the 1 October 2009, Messina, Sicily, event in southern Italy

    NASA Astrophysics Data System (ADS)

    Ardizzone, Francesca; Cardinali, Mauro; Fiorucci, Federica; Iovine, Giulio; Mondini, Alessandro; Reichenbach, Paola; Rossi, Mauro; Teresa, Oreste; Fausto, Guzzetti

    2010-05-01

    In Italy, severe meteorologically induced geo-hydrological events are characterized by a complex combination of landslides and floods, and may cause casualties and damage to urban areas and the utility network. On 1 October 2009, a high intensity rainstorm in the Messina area, Sicily, triggered more than 500 shallow landslides in an area of about 60 km2, mostly in the soils mantling the metamorphic and crystalline bedrock of the Peloritan Arc. The high intensity rainfall further resulted in massive erosion and deposition of debris along the ephemeral drainage channels, widespread inundation, and local modification of the coastline. Damage was particularly severe in the several small villages present in the area, including Giampilieri, Scaletta Zanclea, Guidomandri, Pèzzolo, Altolìa, and Itàla. Damage to the transportation network was also severe and widespread. The several rainfall-induced landslides and the inundations have resulted in 31 deaths, 6 missing persons, numerous injured persons, and more than 2500 evacuated and homeless people. In the aftermath of the event, we: (i) completed a preliminary field survey in the area most affected by landslides and inundations, documenting the ground effects of the intense rainfall, (ii) acquired satellite imagery, including very-high-resolution optical images taken by QuickBird and high-resolution radar images taken by COSMO-SkyMed, and (iii) acquired stereoscopic aerial photography, including pre-event aerial photographs taken in 1954, 1995, and 2005, and post event, very-large scale images taken by helicopter immediately after the event. In this work, we present preliminary results of the exploitation of multiple remote-sensing technologies and information for the identification, mapping and classification of the rainfall induced landslides, and of the eroded and the inundated areas. Emphasis is given to the critical analysis of the capacity and limits of the available airborne and satellite remote sensing

  7. Evaluation of shallow landslide-triggering scenarios through a physically based approach: an example of application in the southern Messina area (northeastern Sicily, Italy)

    NASA Astrophysics Data System (ADS)

    Schilirò, L.; Esposito, C.; Scarascia Mugnozza, G.

    2015-09-01

    Rainfall-induced shallow landslides are a widespread phenomenon that frequently causes substantial damage to property, as well as numerous casualties. In recent~years a wide range of physically based models have been developed to analyze the triggering process of these events. Specifically, in this paper we propose an approach for the evaluation of different shallow landslide-triggering scenarios by means of the TRIGRS (transient rainfall infiltration and grid-based slope stability) numerical model. For the validation of the model, a back analysis of the landslide event that occurred in the study area (located SW of Messina, northeastern Sicily, Italy) on 1 October 2009 was performed, by using different methods and techniques for the definition of the input parameters. After evaluating the reliability of the model through comparison with the 2009 landslide inventory, different triggering scenarios were defined using rainfall values derived from the rainfall probability curves, reconstructed on the basis of daily and hourly historical rainfall data. The results emphasize how these phenomena are likely to occur in the area, given that even short-duration (1-3 h) rainfall events with a relatively low return period (e.g., 10-20~years) can trigger numerous slope failures. Furthermore, for the same rainfall amount, the daily simulations underestimate the instability conditions. The high susceptibility of this area to shallow landslides is testified by the high number of landslide/flood events that have occurred in the past and are summarized in this paper by means of archival research. Considering the main features of the proposed approach, the authors suggest that this methodology could be applied to different areas, even for the development of landslide early warning systems.

  8. Diet and trophic ecology of the lanternfish Electrona risso (Cocco 1829) in the Strait of Messina (central Mediterranean Sea) and potential resource utilization from the Deep Scattering Layer (DSL)

    NASA Astrophysics Data System (ADS)

    Battaglia, Pietro; Andaloro, Franco; Esposito, Valentina; Granata, Antonia; Guglielmo, Letterio; Guglielmo, Rosanna; Musolino, Simona; Romeo, Teresa; Zagami, Giacomo

    2016-07-01

    The feeding habits of the mesopelagic lanternfish Electrona risso from the Strait of Messina (central Mediterranean Sea) were analyzed for the first time. A total of 326 individuals were collected stranded along the Sicilian coast of the Strait of Messina from October 2012 to May 2013. Specimens ranged from 12.0 to 53.8 mm LS (mean LS = 38.6 ± 8.4 mm). Their stomach content was examined and prey composition and feeding strategy were investigated. The results indicate that E. risso is a specialist predator, which feeds mainly on the small mesopelagic fish Cyclothone braueri (%IRI = 74.06) and in minor proportion on zooplankton, with a prevalence of copepods. The specialized feeding strategy of E. risso is confirmed by the low value of Levins standardized index (Bj = 0.141), which indicated a restricted niche breadth. The value of the index of trophic level (TROPH) for E. risso resulted 4.20. The prey composition suggests that E. risso can be considered a weakly vertical migrating species, that feeds on the DSL crustacean and fish communities below 300 m of depth.

  9. Low-Frequency Seismic Ground Motion At The Pier Positions Of The Planned Messina Straits Bridge For A Realistic Earthquake Scenario

    SciTech Connect

    Gusev, A. A.; Pavlov, V.; Romanelli, F.; Panza, G.

    2008-07-08

    We estimated longer-period (period T>0.5 s) components of the ground motion at the piers of the planned Messina straits bridge. As the shortest fault-to-site distance is only 3-5 km, the kinematic earthquake rupture process has to be described in a realistic way and thus, the causative fault is represented by a dense grid of subfaults. To model the 1908 event, we assume a Mw = 7 earthquake, with a 40x20 km rectangular fault, and pure reverse dip-slip. The horizontal upper side of the rectangle is at 3-km depth, and the N corner of the rectangle is just between the piers. For the fault nucleation point, the least favorable place is assumed and a randomized rupture velocity is used in a particular run. In a typical simulation, the fault motion is initially represented by the time history of slip in each of the subfaults and by the distribution of the final seismic moment among the subsources (forming 'asperities'), both generated as lognormal random functions. The time histories are then filtered in order to fit a chosen source spectral model. The parameters that are conditioning the random functions can be based on the bulk of published fault inversions, or reproduced from an earlier successful attempt to simulate ground motions in the epicentral zone of the 1994, M = 6.7 Northridge, California, earthquake. In the second step of calculations, the Green functions (for each subfault and pier combination) are calculated for a layered halfspace model of the pier foundation stratigraphy, using an advanced Green function calculator, that allows an accurate calculation over the entire relevant frequency band including static terms. Finally, the 3-components of the strong ground motion are obtained at the two piers through convolution and summation over the different subsources. We compare a set of response horizontal velocity spectra (PRV) obtained from our calculations with a reference PRV that is considered as a reasonable upper bound for the possible ground motion near

  10. Tectonics and sedimentation of the Lower and Middle Pleistocene mixed siliciclastic/bioclastic sedimentary successions of the Ionian Peloritani Mts (NE Sicily, Southern Italy): the onset of opening of the Messina Strait

    NASA Astrophysics Data System (ADS)

    Stefano, Agata; Longhitano, Sergio

    2009-03-01

    Biostratigraphic analyses carried out on siliciclastic/bioclastic deposits discontinuously cropping out along the Ionian flank of NE Sicily, indicate that they form two sedimentary events of Early and Middle Pleistocene, respec tively. Vertical facies successions, showing transgressive trends, suggest that sedimentation occurred within semi-enclosed marine embayments, where sublittoral coastal wedges developed on steep ramp-type shelves. Sediments accumulated in shoreface to offshore transitions along steep bottom profiles. This depositional scenario was strongly conditioned by the tectonic activity of the rift zone linking Western Calabria and Eastern Sicily. The effects of glacio-eustatism were also recognized. According to our reconstruction, the study area was controlled by a transfer fault system which affected the coastal margin producing major episodes of uplift and subsidence. Block-faulting was responsible for significant cannibalization and recycling of older deposits during the Middle Pleistocene. Such a tectonic setting can be considered the precursor scenario for the formation of the Messina Strait between Calabria and Sicily. This narrow, linear basin influences the hydrodynamic setting of sublittoral deposits along the Ionian coast of Sicily, giving rise to strong flood/ebb tidal currents. The uppermost part of the Middle Pleistocene succession recognized in the study area is indeed dominated by tide-influenced associations of sedimentary structures which most likely record the first stage of the opening of this `seaway' of the central Mediterranean Sea.

  11. Henrique da Rocha Lima.

    PubMed

    Bernardes Filho, Fred; Avelleira, João Carlos Regazzi

    2015-01-01

    Brazilian physician and researcher Henrique da Rocha Lima was born in 1879 in the city of Rio de Janeiro, where he studied medicine and obtained the degree of M.D. in 1901. He specialized in Clinical Medicine in Germany and was the ambassador in European countries of the scientific medicine that emerged from the Oswaldo Cruz Institute in the early twentieth century. Rocha Lima has discovered the causative agent of typhus and had a major contribution to the studies of yellow fever, Chagas disease, Carrión's disease and histoplasmosis. His genius, his research and his discoveries projected his name, and, with it, the image of Brazil in the international scientific scene. PMID:26131867

  12. Henrique da Rocha Lima*

    PubMed Central

    Bernardes Filho, Fred; Avelleira, João Carlos Regazzi

    2015-01-01

    Brazilian physician and researcher Henrique da Rocha Lima was born in 1879 in the city of Rio de Janeiro, where he studied medicine and obtained the degree of M.D. in 1901. He specialized in Clinical Medicine in Germany and was the ambassador in European countries of the scientific medicine that emerged from the Oswaldo Cruz Institute in the early twentieth century. Rocha Lima has discovered the causative agent of typhus and had a major contribution to the studies of yellow fever, Chagas disease, Carrión’s disease and histoplasmosis. His genius, his research and his discoveries projected his name, and, with it, the image of Brazil in the international scientific scene. PMID:26131867

  13. Leonardo da Vinci and the Downburst.

    NASA Astrophysics Data System (ADS)

    Gedzelman, Stanley David

    1990-05-01

    Evidence from the drawings, experiments, and writings of Leonardo da Vinci are presented to demonstrate that da Vinci recognized and, possibly, discovered the downburst and understood its associated airflow. Other early references to vortex flows resembling downbursts are mentioned.

  14. The major histocompatibility complex genes impact pain response in DA and DA.1U rats.

    PubMed

    Guo, Yuan; Yao, Fan-Rong; Cao, Dong-Yuan; Li, Li; Wang, Hui-Sheng; Xie, Wen; Zhao, Yan

    2015-08-01

    Our recent studies have shown that the difference in basal pain sensitivity to mechanical and thermal stimulation between Dark-Agouti (DA) rats and a novel congenic DA.1U rats is major histocompatibility complex (MHC) genes dependent. In the present study, we further used DA and DA.1U rats to investigate the role of MHC genes in formalin-induced pain model by behavioral, electrophysiological and immunohistochemical methods. Behavioral results showed biphasic nociceptive behaviors increased significantly following the intraplantar injection of formalin in the hindpaw of DA and DA.1U rats. The main nociceptive behaviors were lifting and licking, especially in DA rats (P<0.001 and P<0.01). The composite pain scores (CPS) in DA rats were significantly higher than those in DA.1U rats in both phases of the formalin test (P<0.01). Electrophysiological results also showed the biphasic increase in discharge rates of C and Aδ fibers of L5 dorsal root in the two strains, and the net change of the discharge rate of DA rats was significantly higher than that of DA.1U rats (P<0.05). The mechanical thresholds decreased after formalin injection in both strains (P<0.01), and the net change in the mechanical threshold in DA was greater than that in DA.1U rats (P<0.05). The expression of RT1-B, representation of MHC class II molecule, in laminae I-II of L4/5 spinal cord in DA rats was significantly higher than that in DA.1U rats in the respective experimental group (P<0.05). These results suggested that both DA and DA.1U rats exhibited nociceptive responses in formalin-induced pain model and DA rats were more sensitive to noxious chemical stimulus than DA.1U rats, indicating that MHC genes might contribute to the difference in pain sensitivity. PMID:25861730

  15. Fluorine substitution enhanced photovoltaic performance of a D-A(1)-D-A(2) copolymer.

    PubMed

    Dang, Dongfeng; Chen, Weichao; Yang, Renqiang; Zhu, Weiguo; Mammo, Wendimagegn; Wang, Ergang

    2013-10-18

    A new alternating donor-acceptor (D-A1-D-A2) copolymer containing two electron-deficient moieties, isoindigo and quinoxaline, was synthesized. The photovoltaic performance of this polymer could be improved by incorporating fluorine atoms into the quinoxaline units, resulting in an efficiency of 6.32%. This result highlights the attractive promise of D-A1-D-A2 copolymers for high-performance bulk heterojunction solar cells. PMID:24000353

  16. Autocorrelation descriptor improvements for QSAR: 2DA_Sign and 3DA_Sign.

    PubMed

    Sliwoski, Gregory; Mendenhall, Jeffrey; Meiler, Jens

    2016-03-01

    Quantitative structure-activity relationship (QSAR) is a branch of computer aided drug discovery that relates chemical structures to biological activity. Two well established and related QSAR descriptors are two- and three-dimensional autocorrelation (2DA and 3DA). These descriptors encode the relative position of atoms or atom properties by calculating the separation between atom pairs in terms of number of bonds (2DA) or Euclidean distance (3DA). The sums of all values computed for a given small molecule are collected in a histogram. Atom properties can be added with a coefficient that is the product of atom properties for each pair. This procedure can lead to information loss when signed atom properties are considered such as partial charge. For example, the product of two positive charges is indistinguishable from the product of two equivalent negative charges. In this paper, we present variations of 2DA and 3DA called 2DA_Sign and 3DA_Sign that avoid information loss by splitting unique sign pairs into individual histograms. We evaluate these variations with models trained on nine datasets spanning a range of drug target classes. Both 2DA_Sign and 3DA_Sign significantly increase model performance across all datasets when compared with traditional 2DA and 3DA. Lastly, we find that limiting 3DA_Sign to maximum atom pair distances of 6 Å instead of 12 Å further increases model performance, suggesting that conformational flexibility may hinder performance with longer 3DA descriptors. Consistent with this finding, limiting the number of bonds in 2DA_Sign from 11 to 5 fails to improve performance. PMID:26721261

  17. TBI ADAPTER: traumatic brain injury assessment diagnosis advocacy prevention and treatment from the emergency room--a prospective observational study.

    PubMed

    Ganti, Latha; Daneshvar, Yasamin; Bodhit, Aakash; Ayala, Sarah; Patel, Pratik S; Lottenberg, Lawrence L; York, Donna; Counsell, Colleen; Peters, Keith R

    2015-04-01

    There is no standard treatment algorithm for patients who present to the emergency department (ED) with acute traumatic brain injury (TBI). This is in part because of the heterogeneity of the injury pattern and the patient profile, and the lack of evidence-based guidelines, especially for mild TBI in adults. As TBI is seen more and more frequently in the ED, a standardized assessment would be beneficial in terms of efficiency. The authors present their ED approach to mild TBI evaluation in the ED, along with results to date. These data represent a prospective observational cohort study, where each patient provided individual, written informed consent. PMID:25826342

  18. Virtual observatory publishing with DaCHS

    NASA Astrophysics Data System (ADS)

    Demleitner, M.; Neves, M. C.; Rothmaier, F.; Wambsganss, J.

    2014-11-01

    The Data Center Helper Suite DaCHS is an integrated publication package for building VO and Web services, supporting the entire workflow from ingestion to data mapping to service definition. It implements all major data discovery, data access, and registry protocols defined by the VO. DaCHS in this sense works as glue between data produced by the data providers and the standard protocols and formats defined by the VO. This paper discusses central elements of the design of the package and gives two case studies of how VO protocols are implemented using DaCHS' concepts.

  19. Origin of the DA and non-DA white dwarf stars

    NASA Technical Reports Server (NTRS)

    Shipman, Harry L.

    1989-01-01

    Various proposals for the bifurcation of the white dwarf cooling sequence are reviewed. 'Primordial' theories, in which the basic bifurcation of the white dwarf sequence is rooted in events predating the white dwarf stage of stellar evolution, are discussed, along with the competing 'mixing' theories in which processes occurring during the white dwarf stage are responsible for the existence of DA or non-DA stars. A new proposal is suggested, representing a two-channel scenario. In the DA channel, some process reduces the hydrogen layer mass to the value of less than 10 to the -7th. The non-DA channel is similar to that in the primordial scenario. These considerations suggest that some mechanism operates in both channels to reduce the thickness of the outermost layer of the white dwarf. It is also noted that accretion from the interstellar medium has little to do with whether a particular white dwarf becomes a DA or a non-DA star.

  20. Measurement of D->A Momentum Aperture and Test of D->A Field Qualities

    SciTech Connect

    Halling, Mike

    1992-02-27

    The data presented here were taken during two shifts dedicated to D->A studies. The goal during both of these study periods was a test of the field quality of the D->A channel devices, but for the first study period on 10/21/91 the TBT system was not operational so we simply measured the transfer efficiency as a function of momentum. The conclusion from these measurements is as follows: (1) The momentum aperture of the D->A channel is smaller than expected. (2) The restriction is in physical space is somewhere near A:IKIK. (3) The field quality of the injection channel devices is adequate.

  1. VizieR Online Data Catalog: RACE-OC project: YSOs within 100pc (Messina+, 2010)

    NASA Astrophysics Data System (ADS)

    Messina, S.; Desidera, S.; Tutatto, M.; Lanzafame, A. C.; Guinan, E. F.

    2010-11-01

    Rotational periods of stars exhibiting rotational modulation due to photospheric magnetic activity (i.e., starspots) were determined by applying the Lomb-Scargle periodogram technique to photometric time-series data obtained by the All Sky Automated Survey (ASAS). The magnetic activity level was derived from the amplitude of the V lightcurves. The statistical significance of the rotational evolution at different ages was inferred by applying a two-sided Kolmogorov-Smirnov test to subsequent age-bins. (3 data files).

  2. VizieR Online Data Catalog: RACE-OC project: M11 (NGC6705) (Messina+, 2010)

    NASA Astrophysics Data System (ADS)

    Messina, S.; Padmakar, Parihar; Koo, J. R.; Kim, S.-L.; Rey, S.-C.; Lee, C.-U.

    2009-11-01

    In Table 1, we summarise the results of our period search analysis. In Table 2 we give the time series averaged V-band magnitudes (non corrected for interstellar reddening) for the periodic variables newly discovered in the FoV of M11. (2 data files).

  3. The local effect of octreotide on mechanical pain sensitivity is more sensitive in DA rats than DA.1U rats.

    PubMed

    Yao, Fan-Rong; Wang, Hui-Sheng; Guo, Yuan; Zhao, Yan

    2016-02-01

    A recent study by the authors indicated that major histocompatibility complex (MHC) genes are associated with the differences in basal pain sensitivity and in formalin model between Dark-Agouti (DA) and novel congenic DA.1U rats, which have the same genetic background as DA rats except for the u alleles of MHC. The objective of the present study is to investigate whether there is a difference in the pristane-induced arthritis (PIA) model and local analgesic effect of octreotide (OCT) between DA and DA.1U rats. The hindpaw mechanical withdrawal threshold (MWT) and heat withdrawal latency (HWL) were observed. The C unit firings of the tibial nerve evoked by non-noxious and noxious toe movements were recorded by electrophysiological methods in normal and PIA models in DA and DA.1U rats before and after local OCT administration. The expression of somatostatin receptor 2A (SSTR2A) was observed by immunohistochemistry. The results demonstrate that DA rats have a higher mechanical sensitivity than DA.1U rats after PIA. Local OCT administration significantly elevated MWT in DA rats under normal and PIA sate, but not in DA.1U rats. The electrophysiological experiments showed OCT significantly attenuated the firings of C units evoked by non-noxious and noxious stimulation in DA rats more than those in DA.1U rats both in normal and PIA states. In addition, the expression of SSTR2A in the dorsal horn of the spinal cord was significantly higher in DA than in DA.1U rats. All of the findings suggest a higher local analgesic effect of OCT in DA rats than DA.1U rats, which might be associated with the MHC genes. PMID:26606866

  4. Hidden sketches by Leonardo da Vinci revealed

    NASA Astrophysics Data System (ADS)

    Dumé, Belle

    2009-02-01

    Three drawings on the back of Leonardo da Vinci's The Virgin and Child with St Anne (circa 1508) have been discovered by researchers led by Michel Menu from the Centre de Recherche et de Restauration des Musées de France (C2RMF) and the Louvre Museum in Paris.

  5. Common colds on Tristan da Cunha

    PubMed Central

    Shibli, M.; Gooch, S.; Lewis, H. E.; Tyrrell, D. A. J.

    1971-01-01

    Eight epidemics of respiratory disease have been observed among islanders of Tristan da Cunha. They seem to be initiated by the arrival of ships and transmission seemed to occur as a result of close human contact but could not always be traced. Islanders suffered from less colds than those in less isolated communities. PMID:5282927

  6. The PAN-DA data acquisition system

    SciTech Connect

    Petravick, D.; Berg, D.; Berman, E.; Bernett, M.; Constanta-Fanourakis, P.; Dorries, T.; Haire, M.; Kaczar, K; MacKinnon, B.; Moore, C.; Nicinski, T.; Oleynik, G.; Pordes, R.; Sergey, G.; Votava, M.; White, V.

    1989-05-01

    The Online and Data Acquisition software groups at Fermi National Accelerator Laboratory have extended the VAXONLINE data acquisition package to include a VME based data path. The resulting environment, PAN-DA, provides a high throughput for logging, filtering, formatting and selecting events. 10 refs., 1 fig.

  7. How to Think Like Leonardo da Vinci

    ERIC Educational Resources Information Center

    Caouette, Ralph

    2008-01-01

    To be effective and relevant in twenty-first-century learning, art needs to be more inclusive. In this article, the author discusses how teachers can find a good example in Leonardo da Vinci for building an art program. His art, design, and curiosity are the perfect foundation for any art program, at any level. (Contains 3 resources and 3 online…

  8. 40 CFR 60.51Da - Reporting requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Generating Units § 60.51Da Reporting requirements. (a) For SO2, NOX, PM, and NOX plus CO emissions, the... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Reporting requirements. 60.51Da Section 60.51Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS...

  9. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Commercial demonstration permit. 60.47Da Section 60.47Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... Steam Generating Units § 60.47Da Commercial demonstration permit. (a) An owner or operator of...

  10. 40 CFR 60.51Da - Reporting requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Generating Units § 60.51Da Reporting requirements. (a) For SO2, NOX, PM, and NOX plus CO emissions, the... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Reporting requirements. 60.51Da Section 60.51Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS...

  11. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Commercial demonstration permit. 60.47Da Section 60.47Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... Steam Generating Units § 60.47Da Commercial demonstration permit. (a) An owner or operator of...

  12. 40 CFR 60.51Da - Reporting requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Generating Units § 60.51Da Reporting requirements. (a) For SO2, NOX, PM, and NOX plus CO emissions, the... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Reporting requirements. 60.51Da Section 60.51Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS...

  13. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Commercial demonstration permit. 60.47Da Section 60.47Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... Steam Generating Units § 60.47Da Commercial demonstration permit. (a) An owner or operator of...

  14. 32 CFR 516.25 - DA Form 4.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.25 DA Form 4. (a) General. The DA Form 4 (See figure...

  15. A Day in the Life at DaVita Academy

    ERIC Educational Resources Information Center

    Weinstein, Margery

    2010-01-01

    When a company name means "giving life," the bar for learning and development programs is held high. In this article, the author describes what it takes to graduate from DaVita Academy, the soft skills training program dialysis services company DaVita offers all its employees. DaVita's chief executive officer, Kent Thiry, states that the Academy…

  16. 32 CFR 516.25 - DA Form 4.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.25 DA Form 4. (a) General. The DA Form 4 (See figure...

  17. The DA{phi}NE-Light Facility

    SciTech Connect

    Burattini, Emilio; Cinque, Gianfelice; Dabagov, Sultan; Grilli, Antonio; Marcelli, Augusto; Pace, Elisabetta; Piccinini, Massimo; Raco, Agostino; Monti, Francesca

    2004-05-12

    The new Synchrotron Radiation facility at Frascati exploits the intense photon emission from DA{phi}NE, the 0,51 GeV storage ring circulating over 1 A of electrons. Among the three beamlines commissioned, the Synchrotron INfrared Beamline At Da{phi}ne (SINBAD) is fully operational by a brilliant SR beam spanning the entire IR. Recently, the soft X-ray beamline has been characterized and, once implemented the double-crystal monocromator, X-ray Absorption Spectroscopy is applied on material standards in the distinguishing energy region below 4 keV. An UltraViolet line, presently dedicated to photobiology dosimetry, has also given first results on cell irradiation in the UVB band.

  18. The Real Code of Leonardo da Vinci

    PubMed Central

    Ose, Leiv

    2008-01-01

    Leonardo da Vinci was born in Italy. Among the researchers and scientists, he is favourably known for his remarkable efforts in scientific work. His investigations of atherosclerosis judiciously combine three separate fields of research. In 1506, he finished his masterpiece, painting of Mona Lisa. A careful clinical examination of the famous painting reveals a yellow irregular leather-like spot at the inner end of the left upper eyelid and a soft bumpy well-defined swelling of the dorsum of the right hand beneath the index finger about 3 cm long. This is probably the first case of familial hypercholesterolemia (FH). The FH code of Leonardo da Vinci was given immense consideration by scientists like Carl Muller, who described the xanthomas tuberosum and angina pectoris. On the contrary, Akira Endo searched for microbial metabolites that would inhibit HMG-CoA reductase, the rate-limiting enzyme in the synthesis of cholesterol and finally, Michael Brown and Joseph Goldstein published a remarkable series of elegant and insightful papers in the 70s and 80s. They established that the cellular uptake of low-density lipoprotein (LDL) essentially requires the LDL receptor. In conclusion: this was the real Code of Leonardo da Vinci. PMID:19924278

  19. Arnaldo Da Villanova medieval physician (1235-1311). A first approach.

    PubMed

    Ricciardi, Biagio; Ricciardi, Elisabetta; Ricciardi, Carlo Alberto

    2016-02-01

    Arnaldo de Villanova, was a Catalan Physician, born in Villanova de Grau, a suburb of Valencia - Spain about 1235. He died off the coast of Genoa in 1311 during a sea voyage departing from Messina in Sicily, during a diplomatic mission by Pope Clement V in Avignon on orders by the King of Sicily. He was a so famous and clever scientist of the thirteenth century, to give his name to the Universitary Hospital of Montpellier - France. His interests ranged from theology, to politics, medicine, and anymore alchemy. He was an adviser and physician of Kings of Aragon, like Peter III the Great (1276-1285) and James II the Right (1285-1327), of Robert of Angi (1309-1343) of Naples, and of Popes, like Innocenzo V (1276), Bonifacio VIII (1294-1303), Benedetto XI (1303-1304), Clemente V (1305-1314), and of the King of Sicily Federico II of Aragon (1296-1337). For the Pope Bonifacio VIII, suffering from renal colic due to kidney stones, he prescribed Hydrotherapy with Fiuggi Thermal water, that was specially transported for him from its source to Rome and Anagni, in jars wrapped in coarse carpets or wool fabrics, to better maintain the source temperature. In addition in July of 1301, he also produced an astrological seal (Talisman) made of gold loaded of virtues, obtained exposing the seal to the power of the Sun, in those days in the Leo Constellation. This seal was worn by the Pope in an hernial belt of leather to support the kidney,probably to improve hisnephroptosis. Arnaldo produced this seal according to what was described in the book Picatrix - The goal of the wise of the Arabic astronomer and alchemist "Abū l-Qāsim Maslama b.- Ahmad al-Majriti, known with the pseudonym Ghayat al hakim died in Cordova about 1008. Ten years later, after his mysterious death at sea on a Sicilian royal ship, his body was not buried at sea, but was reported in Sicily and buried in the Federician Castle of Montalbano of Elicona at the end of Peloritans Mountains near Milazzo, about 90 km

  20. Asteroid 2012 DA14 To Whiz Past Earth Safely

    NASA Video Gallery

    The small near-Earth asteroid 2012 DA14 will pass very close to Earth on Feb. 15, 2013. Asteroid 2012 DA14 will be closest to Earth at about 11:24 a.m. PST (2:24 p.m. EST and 1924 UTC), on Feb. 15,...

  1. 40 CFR 60.52Da - Recordkeeping requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Recordkeeping requirements. 60.52Da Section 60.52Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Electric Utility Steam Generating Units for...

  2. The Case: Bunche-Da Vinci Learning Partnership Academy

    ERIC Educational Resources Information Center

    Eisenberg, Nicole; Winters, Lynn; Alkin, Marvin C.

    2005-01-01

    The Bunche-Da Vinci case described in this article presents a situation at Bunche Elementary School that four theorists were asked to address in their evaluation designs (see EJ791771, EJ719772, EJ791773, and EJ792694). The Bunche-Da Vinci Learning Partnership Academy, an elementary school located between an urban port city and a historically…

  3. Da Costa's syndrome or neurocirculatory asthenia.

    PubMed Central

    Paul, O

    1987-01-01

    The syndrome variously called Da Costa's syndrome, effort syndrome, neurocirculatory asthenia, etc has been studied for more than 100 years by many distinguished physicians. Originally identified in men in wartime, it has been widely recognised as a common chronic condition in both sexes in civilian life. Although the symptoms may seem to appear after infections and various physical and psychological stresses, neurocirculatory asthenia is most often encountered as a familial disorder that is unrelated to these factors, although they may aggravate an existing tendency. Respiratory complaints (including breathlessness, with and without effort, and smothering sensations) are almost universal, and palpitation, chest discomfort, dizziness and faintness, and fatigue are common. The physical examination is normal. The aetiology is obscure but patients usually have a normal life span. Reassurance and measures to improve physical fitness are helpful. PMID:3314950

  4. 75 FR 52292 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Diamond Aircraft... new airworthiness directive (AD) for all Diamond Aircraft Industries GmbH Models DA 40 and DA...

  5. 75 FR 75868 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-07

    ... Register on August 25, 2010 (75 FR 52292). That NPRM proposed to require a retrofit of the rear passenger... 12866, (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034... Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes AGENCY: Federal Aviation Administration,...

  6. Metal Lines in DA White Dwarfs

    NASA Astrophysics Data System (ADS)

    Zuckerman, B.; Koester, D.; Reid, I. N.; Hünsch, M.

    2003-10-01

    We report Keck telescope HIRES echelle observations of DA white dwarfs in a continuation of an extensive search for metals. These spectra are supplemented with new JHK magnitudes that are used to determine improved atmospheric parameters. Of the DA white dwarfs not in binary or common proper motion systems, about 25% show Ca II lines. For these, Ca abundances are determined from comparison with theoretical equivalent widths from model atmosphere calculations; in a few cases we also obtain Mg, Fe, Si, and Al abundances. If Ca is not observed, we generally determine very stringent upper limits. We compare the data to predictions of previously published models involving the accretion/diffusion of interstellar matter and of comets. The derived abundances are not obviously compatible with the predictions of either model, which up to now could only be tested with traces of metals in helium-rich white dwarfs. By modifying certain assumptions in the published interstellar accretion model we are able to match the distribution of the elements in the white dwarf atmospheres, but, even so, tests of other expectations from this scenario are less successful. Because comet accretion appears unlikely to be the primary cause of the DAZ phenomenon, the data suggest that no more than about 20% of F-type main-sequence stars are accompanied by Oort-like comet clouds. This represents the first observational estimate of this fraction. A plausible alternative to the accretion of cometary or interstellar matter is disruption and accretion of asteroidal material, a model first suggested in 1990 to explain excess near-infrared emission from the DAZ G29-38. An asteroidal debris model to account for the general DAZ phenomenon does not presently disagree with the HIRES data, but neither is there any compelling evidence in support of such a model. The HIRES data indicate that in close red dwarf/white dwarf binaries not known to be cataclysmic variables there is, nonetheless, significant mass

  7. Der Telemanipulator daVinci als mechanisches Trackingsystem

    NASA Astrophysics Data System (ADS)

    Käst, Johannes; Neuhaus, Jochen; Nickel, Felix; Kenngott, Hannes; Engel, Markus; Short, Elaine; Reiter, Michael; Meinzer, Hans-Peter; Maier-Hein, Lena

    Der Telemanipulator daVinci (Intuitive Surgical, Sunnyvale, Kalifornien) ist ein M aster-Slave System für roboterassistierte minimalinvasive Chirurgie. Da er über integrierte Gelenksensoren verfügt, kann er unter Verwendung der daVinci-API als mechanisches Trackingsystem verwendet werden. In dieser Arbeit evaluieren wir die Präzision und Genauigkeit eines daVinci mit Hilfe eines Genauigkeitsphantoms mit bekannten Maßen. Der ermittelte Positionierungsfehler liegt in der Größenordnung von 6 mm und ist somit für einen Großteil der medizinischen Fragestellungen zu hoch. Zur Reduktion des Fehlers schlagen wir daher eine Kalibrierung der Gelenksensoren vor.

  8. Tree Branching: Leonardo da Vinci's Rule versus Biomechanical Models

    PubMed Central

    Minamino, Ryoko; Tateno, Masaki

    2014-01-01

    This study examined Leonardo da Vinci's rule (i.e., the sum of the cross-sectional area of all tree branches above a branching point at any height is equal to the cross-sectional area of the trunk or the branch immediately below the branching point) using simulations based on two biomechanical models: the uniform stress and elastic similarity models. Model calculations of the daughter/mother ratio (i.e., the ratio of the total cross-sectional area of the daughter branches to the cross-sectional area of the mother branch at the branching point) showed that both biomechanical models agreed with da Vinci's rule when the branching angles of daughter branches and the weights of lateral daughter branches were small; however, the models deviated from da Vinci's rule as the weights and/or the branching angles of lateral daughter branches increased. The calculated values of the two models were largely similar but differed in some ways. Field measurements of Fagus crenata and Abies homolepis also fit this trend, wherein models deviated from da Vinci's rule with increasing relative weights of lateral daughter branches. However, this deviation was small for a branching pattern in nature, where empirical measurements were taken under realistic measurement conditions; thus, da Vinci's rule did not critically contradict the biomechanical models in the case of real branching patterns, though the model calculations described the contradiction between da Vinci's rule and the biomechanical models. The field data for Fagus crenata fit the uniform stress model best, indicating that stress uniformity is the key constraint of branch morphology in Fagus crenata rather than elastic similarity or da Vinci's rule. On the other hand, mechanical constraints are not necessarily significant in the morphology of Abies homolepis branches, depending on the number of daughter branches. Rather, these branches were often in agreement with da Vinci's rule. PMID:24714065

  9. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Emission monitoring. 60.49Da Section 60... Generating Units § 60.49Da Emission monitoring. (a) An owner or operator of an affected facility subject...

  10. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Emission monitoring. 60.49Da Section 60... Generating Units for Which Construction is Commenced After September 18, 1978 § 60.49Da Emission...

  11. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Emission monitoring. 60.49Da Section 60... Generating Units § 60.49Da Emission monitoring. (a) An owner or operator of an affected facility subject...

  12. Migration of ATLAS PanDA to CERN

    NASA Astrophysics Data System (ADS)

    Stewart, Graeme Andrew; Klimentov, Alexei; Koblitz, Birger; Lamanna, Massimo; Maeno, Tadashi; Nevski, Pavel; Nowak, Marcin; Emanuel De Castro Faria Salgado, Pedro; Wenaus, Torre

    2010-04-01

    The ATLAS Production and Distributed Analysis System (PanDA) is a key component of the ATLAS distributed computing infrastructure. All ATLAS production jobs, and a substantial amount of user and group analysis jobs, pass through the PanDA system, which manages their execution on the grid. PanDA also plays a key role in production task definition and the data set replication request system. PanDA has recently been migrated from Brookhaven National Laboratory (BNL) to the European Organization for Nuclear Research (CERN), a process we describe here. We discuss how the new infrastructure for PanDA, which relies heavily on services provided by CERN IT, was introduced in order to make the service as reliable as possible and to allow it to be scaled to ATLAS's increasing need for distributed computing. The migration involved changing the backend database for PanDA from MySQL to Oracle, which impacted upon the database schemas. The process by which the client code was optimised for the new database backend is discussed. We describe the procedure by which the new database infrastructure was tested and commissioned for production use. Operations during the migration had to be planned carefully to minimise disruption to ongoing ATLAS offline computing. All parts of the migration were fully tested before commissioning the new infrastructure and the gradual migration of computing resources to the new system allowed any problems of scaling to be addressed.

  13. PKC phosphorylates residues in the N-terminal of the DA transporter to regulate amphetamine-induced DA efflux.

    PubMed

    Wang, Qiang; Bubula, Nancy; Brown, Jason; Wang, Yunliang; Kondev, Veronika; Vezina, Paul

    2016-05-27

    The DA transporter (DAT), a phosphoprotein, controls extracellular dopamine (DA) levels in the central nervous system through transport or reverse transport (efflux). Multiple lines of evidence support the claim that PKC significantly contributes to amphetamine-induced DA efflux. Other signaling pathways, involving CaMKII and ERK, have also been shown to regulate DAT mediated efflux. Here we assessed the contribution of putative PKC residues (S4, S7, S13) in the N-terminal of the DAT to amphetamine-induced DA efflux by transfecting DATs containing different serine to alanine (S-A) point mutations into DA pre-loaded HEK-293 cells and incubating these cells in amphetamine (2μM). The effects of a S-A mutation at the non-PKC residue S12 and a threonine to alanine (T-A) mutation at the ERK T53 residue were also assessed for comparison. WT-DATs were used as controls. In an initial experiment, we confirmed that inhibiting PKC with Go6976 (130nM) significantly reduced amphetamine-induced DA efflux. In subsequent experiments, cells transfected with the S4A, S12A, S13A, T53A and S4,7,13A mutants showed a reduction in amphetamine-induced DA efflux similar to that observed with Go6976. Interestingly, cells transfected with the S7A mutant, identified by some as a PKC-PKA residue, showed unperturbed WT-DAT levels of amphetamine-induced DA efflux. These results indicate that phosphorylation by PKC of select residues in the DAT N-terminal can regulate amphetamine-induced efflux. PKC can act either independently or in concert with other kinases such as ERK to produce this effect. PMID:27113203

  14. A cosmologia no ensino da geografia

    NASA Astrophysics Data System (ADS)

    Santos, S. C.; Chiaradia, A. P. M.

    2003-08-01

    O principal objetivo deste trabalho é auxiliar o professor de Geografia em sala de aula no ensino de tópicos relacionados com a Cosmologia. A idéia deste trabalho surgiu quando foi constatado que o professor de Geografia tem dificuldades de ensinar este tópico. Esta constatação foi feita por uma das autoras ao lecionar este tópico no ensino fundamental e em discussões com outros professores de Geografia. Da mesma maneira que ocorria desde os tempos mais antigos, os alunos têm muito interesse em conhecer os fenômenos que ocorrem no Cosmo, porém os livros didáticos de Geografia utilizados em sala de aula não são ricos em informações sobre este assunto. Assim, o professor de Geografia tem poucas informações para discutir este assunto em sala de aula e não dá a devida importância para este tópico. Então, foi desenvolvido um material de apoio para professores de Geografia sobre a origem do Universo, sua evolução e seu possível futuro evolutivo segundo as mais recentes teorias, com base em perguntas feitas pelos alunos de ensino fundamental e as informações trazidas nos livros didáticos Não cabe a este material inovar e tão pouco trazer uma metodologia de ensino de Cosmologia. Neste material o professor de Geografia pode encontrará um banco de informações, que constitui no estabelecimento de conceitos, teorias e hipóteses, sobre a Cosmologia, em linguagem simples e de fácil entendimento. Para desenvolvê-lo, foram feitas pesquisas não exaustivas em livros e revistas científicas, compilação e discussão em forma cronológica das teorias aceitas sobre modelos cosmológicos. Portanto, este material será apresentado neste trabalho.

  15. Overview of ATLAS PanDA Workload Management

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Wenaus, T.; Nilsson, P.; Stewart, G. A.; Walker, R.; Stradling, A.; Caballero, J.; Potekhin, M.; Smith, D.; ATLAS Collaboration

    2011-12-01

    The Production and Distributed Analysis System (PanDA) plays a key role in the ATLAS distributed computing infrastructure. All ATLAS Monte-Carlo simulation and data reprocessing jobs pass through the PanDA system. We will describe how PanDA manages job execution on the grid using dynamic resource estimation and data replication together with intelligent brokerage in order to meet the scaling and automation requirements of ATLAS distributed computing. PanDA is also the primary ATLAS system for processing user and group analysis jobs, bringing further requirements for quick, flexible adaptation to the rapidly evolving analysis use cases of the early datataking phase, in addition to the high reliability, robustness and usability needed to provide efficient and transparent utilization of the grid for analysis users. We will describe how PanDA meets ATLAS requirements, the evolution of the system in light of operational experience, how the system has performed during the first LHC data-taking phase and plans for the future.

  16. Overview of ATLAS PanDA Workload Management

    SciTech Connect

    Maeno T.; De K.; Wenaus T.; Nilsson P.; Stewart G. A.; Walker R.; Stradling A.; Caballero J.; Potekhin M.; Smith D.

    2011-01-01

    The Production and Distributed Analysis System (PanDA) plays a key role in the ATLAS distributed computing infrastructure. All ATLAS Monte-Carlo simulation and data reprocessing jobs pass through the PanDA system. We will describe how PanDA manages job execution on the grid using dynamic resource estimation and data replication together with intelligent brokerage in order to meet the scaling and automation requirements of ATLAS distributed computing. PanDA is also the primary ATLAS system for processing user and group analysis jobs, bringing further requirements for quick, flexible adaptation to the rapidly evolving analysis use cases of the early datataking phase, in addition to the high reliability, robustness and usability needed to provide efficient and transparent utilization of the grid for analysis users. We will describe how PanDA meets ATLAS requirements, the evolution of the system in light of operational experience, how the system has performed during the first LHC data-taking phase and plans for the future.

  17. The ATLAS PanDA Monitoring System and its Evolution

    NASA Astrophysics Data System (ADS)

    Klimentov, A.; Nevski, P.; Potekhin, M.; Wenaus, T.

    2011-12-01

    The PanDA (Production and Distributed Analysis) Workload Management System is used for ATLAS distributed production and analysis worldwide. The needs of ATLAS global computing imposed challenging requirements on the design of PanDA in areas such as scalability, robustness, automation, diagnostics, and usability for both production shifters and analysis users. Through a system-wide job database, the PanDA monitor provides a comprehensive and coherent view of the system and job execution, from high level summaries to detailed drill-down job diagnostics. It is (like the rest of PanDA) an Apache-based Python application backed by Oracle. The presentation layer is HTML code generated on the fly in the Python application which is also responsible for managing database queries. However, this approach is lacking in user interface flexibility, simplicity of communication with external systems, and ease of maintenance. A decision was therefore made to migrate the PanDA monitor server to Django Web Application Framework and apply JSON/AJAX technology in the browser front end. This allows us to greatly reduce the amount of application code, separate data preparation from presentation, leverage open source for tools such as authentication and authorization mechanisms, and provide a richer and more dynamic user experience. We describe our approach, design and initial experience with the migration process.

  18. Geomagnetic observations on tristan da cunha, south atlantic ocean

    USGS Publications Warehouse

    Matzka, J.; Olsen, N.; Maule, C.F.; Pedersen, L.W.; Berarducci, A.M.; Macmillan, S.

    2009-01-01

    Few geomagnetic ground observations exist of the Earth's strongest core field anomaly, the South Atlantic Anomaly (SAA). The geomagnetic repeat station on the island Tristan da Cunha, located half-way between South Africa and South America at 37?? 05' S, 12?? 18' W, is therefore of crucial importance. We have conducted several sets of repeat station measurements during magnetically quiet conditions (Kp 2o or less) in 2004. The procedures are described and the results are compared to those from earlier campaigns and to the predictions of various global field models. Features of the local crustal bias field and the solar quiet daily variation are discussed. We also evaluate the benefit of continuous magnetic field recordings from Tristan da Cunha, and argue that such a data set is a very valuable addition to geomagnetic satellite data. Recently, funds were set up to establish and operate a magnetometer station on Tristan da Cunha during the Swarm magnetic satellite mission (2011-2014).

  19. Recent Improvements in the ATLAS PanDA Pilot

    NASA Astrophysics Data System (ADS)

    Nilsson, P.; Caballero Bejar, J.; Compostella, G.; Contreras, C.; De, K.; Dos Santos, T.; Maeno, T.; Potekhin, M.; Wenaus, T.

    2012-12-01

    The Production and Distributed Analysis system (PanDA) in the ATLAS experiment uses pilots to execute submitted jobs on the worker nodes. The pilots are designed to deal with different runtime conditions and failure scenarios, and support many storage systems. This talk will give a brief overview of the PanDA pilot system and will present major features and recent improvements including CernVM File System integration, the job retry mechanism, advanced job monitoring including JEM technology, and validation of new pilot code using the HammerCloud stress-testing system. PanDA is used for all ATLAS distributed production and is the primary system for distributed analysis. It is currently used at over 130 sites worldwide. We analyze the performance of the pilot system in processing LHC data on the OSG, EGI and Nordugrid infrastructures used by ATLAS, and describe plans for its further evolution.

  20. Statistical analysis of heavy metals in Cerastoderma edule glaucum and Venerupis aurea laeta from Ganzirri Lake, Messina (Italy).

    PubMed

    Di Bella, Giuseppa; Turco, Vincenzo Lo; Potortì, Angela Giorgia; Rando, Rossana; Licata, Patrizia; Dugo, Giacomo

    2013-09-01

    Our study was carried out on two species of clams, Venerupis aurea laeta and Cerastoderma edule glaucum, from Ganzirri Lake considered as "environmental biomarkers" for their changes in physiology, morphology or distribution under the influence of substances in the environment. The aim of the present study was to conduct a statistical analysis on Venerupis and Cerastoderma to investigate the difference between the two autochthonous clams according to the presence of metals, and to link metal concentrations to the reproductive cycle of clam during 2009-2010. Metal analysis was carried out with inductively coupled plasma mass spectrometry (ICP-MS). Accuracy and precision were assessed by analyzing the certified standard matrix: mussel tissue NIST SRM 2976. The multivariate analysis was made using the SPSS 13.0 software package for Windows (SPSS Inc., Chicago, IL). In this study, the concentrations of some metals found in clam tissues showed seasonal cycles with higher values in summer than in winter. The significances of metal concentrations differences between Venerupis and Cerastoderma samples were estimated with Mann-Whitney U-test. The concentrations of Ag, As, Cd, Mn, Se and Zn show a significant p-level that suggests a difference between the two group samples. Statistical analysis showed that the link of metal concentrations to the reproductive cycles of Venerupis and Cerastoderma was not evident. PMID:23412955

  1. The influence of land use change on landslide susceptibility zonation: the Briga catchment test site (Messina, Italy).

    PubMed

    Reichenbach, P; Busca, C; Mondini, A C; Rossi, M

    2014-12-01

    The spatial distribution of landslides is influenced by different climatic conditions and environmental settings including topography, morphology, hydrology, lithology, and land use. In this work, we have attempted to evaluate the influence of land use change on landslide susceptibility (LS) for a small study area located in the southern part of the Briga catchment, along the Ionian coast of Sicily (Italy). On October 1, 2009, the area was hit by an intense rainfall event that triggered abundant slope failures and resulted in widespread erosion. After the storm, an inventory map showing the distribution of pre-event and event landslides was prepared for the area. Moreover, two different land use maps were developed: the first was obtained through a semi-automatic classification of digitized aerial photographs acquired in 1954, the second through the combination of supervised classifications of two recent QuickBird images. Exploiting the two land use maps and different land use scenarios, LS zonations were prepared through multivariate statistical analyses. Differences in the susceptibility models were analyzed and quantified to evaluate the effects of land use change on the susceptibility zonation. Susceptibility maps show an increase in the areal percentage and number of slope units classified as unstable related to the increase in bare soils to the detriment of forested areas. PMID:25164982

  2. The role of transparency in da Vinci stereopsis.

    PubMed

    Zannoli, Marina; Mamassian, Pascal

    2011-10-15

    The majority of natural scenes contains zones that are visible to one eye only. Past studies have shown that these monocular regions can be seen at a precise depth even though there are no binocular disparities that uniquely constrain their locations in depth. In the so-called da Vinci stereopsis configuration, the monocular region is a vertical line placed next to a binocular rectangular occluder. The opacity of the occluder has been mentioned to be a necessary condition to obtain da Vinci stereopsis. However, this opacity constraint has never been empirically tested. In the present study, we tested whether da Vinci stereopsis and perceptual transparency can interact using a classical da Vinci configuration in which the opacity of the occluder varied. We used two different monocular objects: a line and a disk. We found no effect of the opacity of the occluder on the perceived depth of the monocular object. A careful analysis of the distribution of perceived depth revealed that the monocular object was perceived at a depth that increased with the distance between the object and the occluder. The analysis of the skewness of the distributions was not consistent with a double fusion explanation, favoring an implication of occlusion geometry in da Vinci stereopsis. A simple model that includes the geometry of the scene could account for the results. In summary, the mechanism responsible to locate monocular regions in depth is not sensitive to the material properties of objects, suggesting that da Vinci stereopsis is solved at relatively early stages of disparity processing. PMID:21906614

  3. Situação da Mulher na Astronomia Brasileira

    NASA Astrophysics Data System (ADS)

    Silva, Adriana V. R.

    2007-07-01

    O conteúdo desse texto surgiu de uma apresentação de mesmo título que fiz na XXXI Reunião Anual da Sociedade Astronômica Brasileira (SAB) em 2005. Esse tema foi inspirado originalmente pela minha participação no "2nd UIPAP International Conference on Women in Physics" realizado entre 23 e 25 de maio de 2005 no Rio de Janeiro. Essa é uma conferência internacional que acontece de três em três anos, sendo que a primeira ocorreu em 2002 na cidade de Paris, França. Participei dessa conferência como membro da delegação da Sociedade Brasileira de Física e um dos trabalhos que apresentei versava sobre a situação das mulheres na Astronomia brasileira, cujos resultados principais discorro a seguir. A situação das astrônomas, baseada nos dados dos sócios da SAB coletados no final de 2004, é comparada com a das físicas brasileiras e também com as nossas colegas americanas. Os dados identificam ainda uma maior evasão da carreira por parte das mulheres do que os homens. Alguns dos possíveis motivos da evasão são discutidos, como o desejo de constituir família e/ou isolamento. Resultados um tanto preocupantes com relação à distribuição de bolsas de produtividade do CNPq também são apresentados. As principais discussões e estratégias recomendadas nesse congresso são mencionadas de forma resumida ao final.

  4. The future of PanDA in ATLAS distributed computing

    NASA Astrophysics Data System (ADS)

    De, K.; Klimentov, A.; Maeno, T.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.

    2015-12-01

    Experiments at the Large Hadron Collider (LHC) face unprecedented computing challenges. Heterogeneous resources are distributed worldwide at hundreds of sites, thousands of physicists analyse the data remotely, the volume of processed data is beyond the exabyte scale, while data processing requires more than a few billion hours of computing usage per year. The PanDA (Production and Distributed Analysis) system was developed to meet the scale and complexity of LHC distributed computing for the ATLAS experiment. In the process, the old batch job paradigm of locally managed computing in HEP was discarded in favour of a far more automated, flexible and scalable model. The success of PanDA in ATLAS is leading to widespread adoption and testing by other experiments. PanDA is the first exascale workload management system in HEP, already operating at more than a million computing jobs per day, and processing over an exabyte of data in 2013. There are many new challenges that PanDA will face in the near future, in addition to new challenges of scale, heterogeneity and increasing user base. PanDA will need to handle rapidly changing computing infrastructure, will require factorization of code for easier deployment, will need to incorporate additional information sources including network metrics in decision making, be able to control network circuits, handle dynamically sized workload processing, provide improved visualization, and face many other challenges. In this talk we will focus on the new features, planned or recently implemented, that are relevant to the next decade of distributed computing workload management using PanDA.

  5. Publishing Planetary Data to the VO using DaCHS

    NASA Astrophysics Data System (ADS)

    Demleitner, M.

    2014-04-01

    Publishing to the Virtual Observatory has many advantages, but between standards compliance, registration, data organisation, and server operation it is also requires a nontrivial effort if starting from scratch. The DaCHS software from the German Astrophysical Virtual Observatory offers a packaged solution letting publishers concentrate on working with their own data and metadata as much as possible. For the planetary community, publishing is further facilitated by built-in support for the EPN-TAP data model. This contribution will show a typical publishing workflow together with a brief overview of how DaCHS meshes in with the VO and Web-based data usage.

  6. Asteroid 1986 DA - Radar evidence for a metallic composition

    NASA Technical Reports Server (NTRS)

    Ostro, S. J.; Rosema, K. D.; Campbell, D. B.; Chandler, J. F.; Hine, A. A.; Hudson, R. S.

    1991-01-01

    Echoes from the near-earth object 1986 DA show it to be significantly more reflective than other radar-detected asteroids. This result supports the hypothesis that 1986 DA is a piece of NiFe metal derived from the interior of a much larger object that melted, differentiated, cooled, and subsequently was disrupted in a catastrophic collision. This 2-kilometer asteroid, which appears smooth at centimeter to meter scales but extremely irregular at 10- to 100-meter scales, might be (or have been a part of) the parent body of some iron meteorites.

  7. Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II.

    PubMed

    Tohri, Akihiko; Dohmae, Naoshi; Suzuki, Takehiro; Ohta, Hisataka; Inoue, Yasunori; Enami, Isao

    2004-03-01

    To elucidate the domains on the extrinsic 23 kDa protein involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II, we modified amino or carboxyl groups of the 23 kDa protein to uncharged methyl ester groups with N-succinimidyl propionate or glycine methyl ester in the presence of a water-soluble carbodiimide, respectively. The N-succinimidyl propionate-modified 23 kDa protein did not bind to the 33 kDa protein associated with PSII membranes, whereas the glycine methyl ester-modified 23 kDa protein completely bound. This indicates that positive charges on the 23 kDa protein are important for electrostatic interaction with the 33 kDa protein associated with the PSII membranes. Mapping of the N-succinimidyl propionate-modified sites of the 23 kDa protein was performed using Staphylococcus V8 protease digestion of the modified protein followed by determination of the mass of the resultant peptide fragments with MALDI-TOF MS. The results showed that six domains (Lys11-Lys14, Lys27-Lys38, Lys40, Lys90-Lys96, Lys143-Lys152, Lys166-Lys174) were modified with N-succinimidyl propionate. In these domains, Lys11, Lys13, Lys33, Lys38, Lys143, Lys166, Lys170 and Lys174 were wholly conserved in the 23 kDa protein from 12 species of higher plants. These positively charged lysyl residues on the 23 kDa protein may be involved in electrostatic interactions with the negatively charged carboxyl groups on the 33 kDa protein, the latter has been suggested to be important for the 23 kDa binding [Bricker, T.M. & Frankel, L.K. (2003) Biochemistry42, 2056-2061]. PMID:15009208

  8. 40 CFR 60.45Da - Standard for mercury (Hg).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Standard for mercury (Hg). 60.45Da... for mercury (Hg). (a) For each coal-fired electric utility steam generating unit other than an IGCC... gases that contain mercury (Hg) emissions in excess of each Hg emissions limit in paragraphs...

  9. Training and Health. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles programs in the fields of health and medicine that are offered through the European Commission's Leonardo da Vinci program. The following programs are profiled: (1) CYTOTRAIN (a transnational vocational training program in cervical cancer screening); (2) Apollo (a program of open and distance learning for paramedical…

  10. The DaVinci Project: Multimedia in Art and Chemistry.

    ERIC Educational Resources Information Center

    Simonson, Michael; Schlosser, Charles

    1998-01-01

    Provides an overview of the DaVinci Project, a collaboration of students, teachers, and researchers in chemistry and art to develop multimedia materials for grades 3-12 visualizing basic concepts in chemistry and visual art. Topics addressed include standards in art and science; the conceptual framework for the project; and project goals,…

  11. The Potential da Vinci in All of Us

    ERIC Educational Resources Information Center

    Petto, Sarah; Petto, Andrew

    2009-01-01

    The study of the human form is fundamental to both science and art curricula. For vertebrates, perhaps no feature is more important than the skeleton to determine observable form and function. As Leonard da Vinci's famous Proportions of the Human Figure (Virtruvian Man) illustrates, the size, shape, and proportions of the human body are defined by…

  12. DATA ASSESSMENT REPORTS FOR CEMS AT SUBPART DA FACILITIES

    EPA Science Inventory

    EPA promulgated minimum quality assurance (QA) requirements for Continuous Emission Monitoring Systems (CEMS) in 40 CFR Part 60 Appendix F. Appendix F requires Da source owners to develop site-specific QA plans and report the results of EPA specified QA activities each calendar q...

  13. Asteroid 1986 DA: Radar evidence for a metallic composition

    NASA Technical Reports Server (NTRS)

    Ostro, Steven J.; Campbell, D. B.; Chandler, J. F.; Hine, A. A.; Hudson, R. S.; Rosema, K. D.; Shapiro, Irwin I.

    1991-01-01

    Radar observations of the near-Earth asteroid 1986 DA were carried out at the Arecibo Observatory in April 1986, two months after its discovery. Radar results are consistent with the hypothesis that 1986 HA is a piece of NiFe metal derived from the interior of a much larger object that melted, differentiated, cooled and subsequently was disrupted in a catastrophic collision. This 2-km asteroid might be (or have been part of) the parent body of some iron meteorites. Or 1986 DA might share the parentage and/or part of the dynamical history of some meteorites without ever having contributed any of its own ejecta to our meteorite sample. Analysis of the samples returned from 1986 DA might ultimately involve economic considerations. Meteoritic metal is mostly iron with about 8 percent nickel, but also contains substantial concentrations of precious and strategic metals, including approx. 1 ppm of gold and approx. 10 ppm of platinum group elements. If these abundances apply to 1986 DA, it contains some 10(exp 16) g of iron, 10 (exp 15) g of nickel, 10(exp 11) g of platinum group metals, and 10(exp 10) g of gold.

  14. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... potential combustion concentration (80 percent reduction) for each 24-hour period of steam generator... owner or operator of a fluidized bed combustion electric utility steam generator (atmospheric or... Steam Generating Units for Which Construction is Commenced After September 18, 1978 § 60.47Da...

  15. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... potential combustion concentration (80 percent reduction) for each 24-hour period of steam generator... owner or operator of a fluidized bed combustion electric utility steam generator (atmospheric or... Steam Generating Units for Which Construction is Commenced After September 18, 1978 § 60.47Da...

  16. Women and Technical Professions. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles programs for women in technical professions that are offered through the European Commission's Leonardo da Vinci program. The following programs are profiled: (1) Artemis and Diana (vocational guidance programs to help direct girls toward technology-related careers); (2) CEEWIT (an Internet-based information and…

  17. Studying and Working Abroad. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles recent successful examples of students studying and working abroad as part of the European Commission's Leonardo da Vinci program, which is designed to give students across the European Union the opportunity to experience vocational training in a foreign country. The following examples are presented: (1) 3 Finnish students…

  18. Modelagem do vento e da fotosfera de AG Carinae

    NASA Astrophysics Data System (ADS)

    Groh, J. H.; Damineli, A.

    2003-08-01

    A trajetória evolutiva das estrelas de alta massa depende fortemente de suas taxas de perda de massa. Apesar do rápido progresso no estudo destas estrelas, a taxa de perda de massa e outros parâmetros físicos básicos, como a temperatura superficial e a velocidade terminal do vento ainda não estão bem determinados. Isto ocorre devido à presença de ventos irregulares, rápidos e fortes ao redor destas estrelas, tornando a interpretação dos seus espectros uma tarefa difícil. Assim, a modelagem do vento e da fotosfera dessas estrelas está sendo cada vez mais usada para obter tais parâmetros a partir dos espectros. O aumento da taxa de perda de massa durante a fase LBV (Variáveis Luminosas Azuis), comparado com outros tipos de estrelas, tem sido atribuído a instabilidades do tipo S Doradus. Dispomos de uma base de dados espectroscópicos cobrindo 22 anos de observações de AG Carinae, incluindo um ciclo S Doradus completo, com espectros CCD em alta resolução na faixa óptica e infravermelha. Utilizamos o programa desenvolvido por Schmutz (1997) para uma análise preliminar desse ciclo, obtendo a taxa de perda de massa a partir da linha do Ha. Não existe uma correlação clara da taxa de perda de massa com mudanças da temperatura efetiva, do raio da estrela e do fluxo na banda V. A estrela atingiu seu mínimo fotométrico (raio mínimo) em 1990 e o máximo fotométrico (raio máximo) em 1995, enquanto que o fluxo máximo da linha do Ha ocorreu em 1996. Além disso a taxa de perda de massa não segue esse ciclo, contrariamente às idéias correntes. Para fazer um modelo mais realista estamos usando o programa CMFGEN (Hillier & Miller), que trata a fotosfera e o vento estelar de forma consistente, considerando a radiação fora do equilíbrio termodinâmico (NLTE) e com blanketting total de linhas. Simulamos o espectro de AG Carinae em duas épocas extremas do ciclo S Dor para testar os resultados obtidos com o modelo mais simplificado.

  19. 49 CFR 178.58 - Specification 4DA welded steel cylinders for aircraft use.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Specification 4DA welded steel cylinders for...) SPECIFICATIONS FOR PACKAGINGS Specifications for Cylinders § 178.58 Specification 4DA welded steel cylinders for aircraft use. (a) Type, size, and service pressure. A DOT 4DA is a welded steel sphere (two...

  20. 49 CFR 178.58 - Specification 4DA welded steel cylinders for aircraft use.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Specification 4DA welded steel cylinders for...) SPECIFICATIONS FOR PACKAGINGS Specifications for Cylinders § 178.58 Specification 4DA welded steel cylinders for aircraft use. (a) Type, size, and service pressure. A DOT 4DA is a welded steel sphere (two...

  1. In vivo breakdown products of the 32 kDa thylakoid herbicide binding protein. [Spirodela oligorrhiza

    SciTech Connect

    Greenberg, B.M.; Mattoo, A.K.; Gaba, V.; Edelman, M.

    1986-04-01

    The 32 kDa herbicide binding protein of PSII is degraded rapidly in the light. This phenomenon was investigated in Spirodela oligorrhiza. When fronds were radiolabeled in the presence of cycloheximide only the chloroplast-encoded proteins were labeled. Under these conditions, a breakdown product of 23.5 kDa was observed. This polypeptide was further degraded with kinetics similar to that of the 32 kDa protein. The 23.5 kDa polypeptide cross-reacted with an antibody specific to the 32 kDa protein. By protease digestion it was determined that the 23.5 kDa polypeptide is the intact N-terminal piece of the 32 kDa protein. Thus, this product corresponds to the membrane anchor of the 32 kDa protein. Using the same antibody, breakdown products of 16 kDa, 14 kDa and 12 kDa were observed. These products have also been observed in Zea mays and Solanum nigrum. Using DCMU during pulse-chase experiments at different light intensities, evidence was obtained that the 23.5 kDa breakdown product is generated in vivo.

  2. Roles and Delegation of Authority (R/DA) System

    SciTech Connect

    ABBOTT,JOHN P.; HUTCHINS,JAMES C.; SCHOCH,DAVID G.

    1999-11-01

    The processes of defining managerial roles and providing for delegation of authority are essential to any enterprise. At most large organizations, these processes are defined in policy manuals and through sets of standard operating procedures for many, if not all, business and administrative functions. Many of these staff-initiated, administrative functions require the routing of documents for approval to one or more levels of management. These employee-oriented, back office types of workflows tend to require more flexibility in determining to whom these documents should go to, while, at the same time, providing the responsible parties with the flexibility to delegate their approval authority or allow others to review their work. Although this practice is commonplace in manual, paper-based processes that exist in many organizations, it is difficult to provide the same flexibility in the more structured, electronic-based, workflow systems. The purpose of this report is to present a framework or architecture for creating a R/DA system and provide some insights associated with its design and utilization. To improve understanding and clarify subsequent discussion, the goals and requirements for the major R/DA system components, namely, the database and interface modules, are initially presented along with the identification of important concepts and the definition of critical terms. Next high-level functions relating the types of inputs to the outputs of the R/DA interface module are introduced and discussed. Then the relationships between the major R/DA modules and the primary components associated with its creation and maintenance are presented and analyzed. Finally, some conclusions are drawn relative to the advantages associated with developing a R/DA system for use in implementing an enterprise-wide, work-facilitating information system.

  3. JCoDA: a tool for detecting evolutionary selection

    PubMed Central

    2010-01-01

    Background The incorporation of annotated sequence information from multiple related species in commonly used databases (Ensembl, Flybase, Saccharomyces Genome Database, Wormbase, etc.) has increased dramatically over the last few years. This influx of information has provided a considerable amount of raw material for evaluation of evolutionary relationships. To aid in the process, we have developed JCoDA (Java Codon Delimited Alignment) as a simple-to-use visualization tool for the detection of site specific and regional positive/negative evolutionary selection amongst homologous coding sequences. Results JCoDA accepts user-inputted unaligned or pre-aligned coding sequences, performs a codon-delimited alignment using ClustalW, and determines the dN/dS calculations using PAML (Phylogenetic Analysis Using Maximum Likelihood, yn00 and codeml) in order to identify regions and sites under evolutionary selection. The JCoDA package includes a graphical interface for Phylip (Phylogeny Inference Package) to generate phylogenetic trees, manages formatting of all required file types, and streamlines passage of information between underlying programs. The raw data are output to user configurable graphs with sliding window options for straightforward visualization of pairwise or gene family comparisons. Additionally, codon-delimited alignments are output in a variety of common formats and all dN/dS calculations can be output in comma-separated value (CSV) format for downstream analysis. To illustrate the types of analyses that are facilitated by JCoDA, we have taken advantage of the well studied sex determination pathway in nematodes as well as the extensive sequence information available to identify genes under positive selection, examples of regional positive selection, and differences in selection based on the role of genes in the sex determination pathway. Conclusions JCoDA is a configurable, open source, user-friendly visualization tool for performing evolutionary

  4. [Lobectomy for lung cancer using the Da Vinci surgical system].

    PubMed

    Nakamura, Hiroshige

    2014-05-01

    Robot-assisted surgery using the da Vinci surgical system has attracted attention because of excellent operability without shaking by joint forceps under the clear vision of a three-dimensional high-definition camera in lung cancer surgery. Although this form of advanced medical care is not yet approved for insurance coverage, it is at the stage of clinical research and expected to be useful in hilar exposure, lymph node dissection, and suturing of the lung parenchyma or bronchus. Lung cancer surgery with the da Vinci system has the advantage of combining thoracotomy and minimally invasive surgery in video-assisted thoracic surgery. However, safety management, education, and significant cost are problems to be resolved. Several important issues such as sharing knowledge and technology of robotic surgery, education, training, development of new instruments, and acquisition of advanced medical insurance are discussed for the future development of robotic surgical systems. PMID:24946522

  5. DaVinci's Mona Lisa entering the next dimension.

    PubMed

    Carbon, Claus-Christian; Hesslinger, Vera M

    2013-01-01

    For several of Leonardo da Vinci's paintings, such as The Virgin and Child with St Anne or the Mona Lisa, there exist copies produced by his own studio. In case of the Mona Lisa, a quite exceptional, rediscovered studio copy was presented to the public in 2012 by the Prado Museum in Madrid. Not only does it mirror its famous counterpart superficially; it also features the very same corrections to the lower layers, which indicates that da Vinci and the 'copyist' must have elaborated their panels simultaneously. On the basis of subjective (thirty-two participants estimated painter-model constellations) as well as objective data (analysis of trajectories between landmarks of both paintings), we revealed that both versions differ slightly in perspective. We reconstructed the original studio setting and found evidence that the disparity between both paintings mimics human binocular disparity. This points to the possibility that the two Giocondas together might represent the first stereoscopic image in world history. PMID:24303752

  6. TA-DA: A TOOL FOR ASTROPHYSICAL DATA ANALYSIS

    SciTech Connect

    Da Rio, Nicola; Robberto, Massimo

    2012-12-01

    We present the Tool for Astrophysical Data Analysis (TA-DA), a new software aimed to greatly simplify and improve the analysis of stellar photometric data in comparison with theoretical models, and allow the derivation of stellar parameters from multi-band photometry. Its flexibility allows one to address a number of such problems: from the interpolation of stellar models, or sets of stellar physical parameters in general, to the computation of synthetic photometry in arbitrary filters or units; from the analysis of observed color-magnitude diagrams to a Bayesian derivation of stellar parameters (and extinction) based on multi-band data. TA-DA is available as a pre-compiled Interactive Data Language widget-based application; its graphical user interface makes it considerably user-friendly. In this paper, we describe the software and its functionalities.

  7. The Da Vinci code dynamically de-coded.

    PubMed

    Cohen, Mariam

    2005-01-01

    The novel The Da Vinci Code, by Dan Brown has been on best-seller lists for over two years. An examination of Brown's previous novels reveals a well-designed plot line shared by all four novels that not only makes them good "thrillers" but also creates a mythological structure to the novels that draws on common unconscious fantasies in the same way that fairy tales do. One aspect of this mythological structure is the use of evil conspiracies (and benign ones as well) for the protagonist to overcome. In addition, The Da Vinci Code presents a religious theme involving legends about Mary Magdalene. This theme touches on the role of a feminine aspect to divinity in allowing for an erotic connection with the divine. PMID:16448349

  8. The da Vinci robot in right adrenalectomy: considerations on technique.

    PubMed

    D'Annibale, Annibale; Fiscon, Valentino; Trevisan, Paolo; Pozzobon, Maurizia; Gianfreda, Valeria; Sovernigo, Gianna; Morpurgo, Emilio; Orsini, Camillo; Del Monte, Daniele

    2004-02-01

    The da Vinci Robotic System (Intuitive Surgical, Mountain View, CA) became available at the General Surgery Department of Camposampiero Hospital in May 2001. From May 2001 to October 2002, 139 robotic operations were performed, one of which was a right adrenalectomy for a right adrenal mass. The progressive growth of the mass was the indication for surgical excision. Surgical adrenalectomy was successfully completed with da Vinci Robotic System using 5 ports (3 for the robotic system, 2 as service trocars). The wrist-like movements of the instrument's tip easily enabled the detachment of the right hepatic lobe from the gland and vessel isolation, while the 3-dimensional vision facilitated dissection of the veins from the vena cava. PMID:15259586

  9. PanDaTox: a tool for accelerated metabolic engineering

    SciTech Connect

    Amitai, Gil; Sorek, Rotem

    2012-04-18

    Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox (www.weizmann.ac.il/pandatox), a web-based resource that provides experimental toxicity information for more than 1.5 million genes from hundreds of different microbial genomes. The toxicity predictions, which were extensively experimentally verified, are based on serial cloning of genes into E. coli as part of the Sanger whole genome shotgun sequencing process. PanDaTox can accelerate metabolic engineering projects by allowing researchers to exclude toxic genes from the engineering plan and verify the clonability of selected genes before the actual metabolic engineering experiments are conducted.

  10. Improving Security in the ATLAS PanDA System

    NASA Astrophysics Data System (ADS)

    Caballero, J.; Maeno, T.; Nilsson, P.; Stewart, G.; Potekhin, M.; Wenaus, T.

    2011-12-01

    The security challenges faced by users of the grid are considerably different to those faced in previous environments. The adoption of pilot jobs systems by LHC experiments has mitigated many of the problems associated with the inhomogeneities found on the grid and has greatly improved job reliability; however, pilot jobs systems themselves must then address many security issues, including the execution of multiple users' code under a common 'grid' identity. In this paper we describe the improvements and evolution of the security model in the ATLAS PanDA (Production and Distributed Analysis) system. We describe the security in the PanDA server which is in place to ensure that only authorized members of the VO are allowed to submit work into the system and that jobs are properly audited and monitored. We discuss the security in place between the pilot code itself and the PanDA server, ensuring that only properly authenticated workload is delivered to the pilot for execution. When the code to be executed is from a 'normal' ATLAS user, as opposed to the production system or other privileged actor, then the pilot may use an EGEE developed identity switching tool called gLExec. This changes the grid proxy available to the job and also switches the UNIX user identity to protect the privileges of the pilot code proxy. We describe the problems in using this system and how they are overcome. Finally, we discuss security drills which have been run using PanDA and show how these improved our operational security procedures.

  11. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... defined in 40 CFR 72.2, may use, as an alternative to the requirements specified in either paragraph (l... § 60.58b(i)(3) of subpart Eb of this part. (ii) Each 1-hour CO emissions average is calculated using... 40 Protection of Environment 6 2011-07-01 2011-07-01 false Emission monitoring. 60.49Da Section...

  12. 40 CFR 60.49Da - Emission monitoring.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... affected unit that qualifies as a gas-fired or oil-fired unit, as defined in 40 CFR 72.2, may use, as an... § 60.58b(i)(3) of subpart Eb of this part. (ii) Each 1-hour CO emissions average is calculated using... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Emission monitoring. 60.49Da Section...

  13. Surgical treatment of parietal defects with "da Vinci" surgical robot.

    PubMed

    Vasilescu, D; Paun, S

    2012-06-12

    The robotic surgery has come through the development of telemedicine and minimally invasive surgery concepts, being developed in the military medicine by NASA during the years 1970-1980. The purpose of this paper is to briefly present our experience in the new field of the robotic surgery, by analyzing the results obtained over a lot of 20 patients operated with the "da Vinci" robot within the last 5 years in the Clinical Emergency Hospital Bucharest for various abdominal defects. PMID:22802899

  14. Identification of an abundant 56 kDa protein implicated in food allergy as granule-bound starch synthase

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rice, the staple food of South and East Asian counties, is considered to be hypoallergenic. However, several clinical studies have documented rice-induced allergy in sensitive patients. Rice proteins with molecular weights of 14-16 kDa, 26 kDa, 33 kDa and 56 kDa have been identified as allergens. Re...

  15. The ATLAS PanDA Pilot in Operation

    NASA Astrophysics Data System (ADS)

    Nilsson, P.; Caballero, J.; De, K.; Maeno, T.; Stradling, A.; Wenaus, T.; ATLAS Collaboration

    2011-12-01

    The Production and Distributed Analysis system (PanDA) [1-2] was designed to meet ATLAS [3] requirements for a data-driven workload management system capable of operating at LHC data processing scale. Submitted jobs are executed on worker nodes by pilot jobs sent to the grid sites by pilot factories. This paper provides an overview of the PanDA pilot [4] system and presents major features added in light of recent operational experience, including multi-job processing, advanced job recovery for jobs with output storage failures, gLExec [5-6] based identity switching from the generic pilot to the actual user, and other security measures. The PanDA system serves all ATLAS distributed processing and is the primary system for distributed analysis; it is currently used at over 100 sites worldwide. We analyze the performance of the pilot system in processing real LHC data on the OSG [7], EGI [8] and Nordugrid [9-10] infrastructures used by ATLAS, and describe plans for its evolution.

  16. Common commercial cosmetic products induce arthritis in the DA rat.

    PubMed Central

    Sverdrup, B; Klareskog, L; Kleinau, S

    1998-01-01

    Many different agents, including mineral oil and silicone, have the capacity to act as immunological adjuvants, i.e., they can contribute to the activation of the immune system. Some adjuvants, including mineral oil, are known to induce arthritis in certain strains of rats after intradermal injection or percutaneous application. The aim of this study was to determine if common commercial cosmetic products containing mineral oil could induce arthritis in the highly susceptible DA (Dark Agouti) rat. Intradermal injection of five out of eight assayed cosmetic products without further additives resulted in arthritis with synovitis. One of the products induced a very aggressive arthritis, which had declined after 5-9 weeks. When this product was also assayed for arthritogenicity upon percutaneous administration, it induced a mild and transient arthritis in 5 out of 10 DA rats, whereas control animals showed no clinical signs of joint involvement. No arthritic reaction was seen in rats after peroral feeding with the most arthritogenic product or by intravaginal application of Freund's adjuvants. Silicone gel implants in DA rats did not cause arthritis. We conclude that mineral oils included in common commercially available products retain their adjuvant properties and are arthritogenic in the presently investigated arthritis-prone rat strain. There is yet no evidence that mineral oils present in cosmetics may contribute to arthritis in humans, but we suggest that this question should be subject to further investigation. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:9417771

  17. OpenDA-NEMO framework for ocean data assimilation

    NASA Astrophysics Data System (ADS)

    van Velzen, Nils; Altaf, Muhammad Umer; Verlaan, Martin

    2016-03-01

    Data assimilation methods provide a means to handle the modeling errors and uncertainties in sophisticated ocean models. In this study, we have created an OpenDA-NEMO framework unlocking the data assimilation tools available in OpenDA for use with NEMO models. This includes data assimilation methods, automatic parallelization, and a recently implemented automatic localization algorithm that removes spurious correlations in the model based on uncertainties in the computed Kalman gain matrix. We have set up a twin experiment where we assimilate sea surface height (SSH) satellite measurements. From the experiments, we can conclude that the OpenDA-NEMO framework performs as expected and that the automatic localization significantly improves the performance of the data assimilation algorithm by successfully removing spurious correlations. Based on these results, it looks promising to extend the framework with new kinds of observations and work on improving the computational speed of the automatic localization technique such that it becomes feasible to include large number of observations.

  18. Amelioration of Auditory Response by DA9801 in Diabetic Mouse

    PubMed Central

    Hong, Bin Na; Her, You Ri; Castañeda, Rodrigo; Moon, Hyo Won

    2015-01-01

    Diabetes mellitus (DM) is a metabolic disease that involves disorders such as diabetic retinopathy, diabetic neuropathy, and diabetic hearing loss. Recently, neurotrophin has become a treatment target that has shown to be an attractive alternative in recovering auditory function altered by DM. The aim of this study was to evaluate the effect of DA9801, a mixture of Dioscorea nipponica and Dioscorea japonica extracts, in the auditory function damage produced in a STZ-induced diabetic model and to provide evidence of the mechanisms involved in enhancing these protective effects. We found a potential application of DA9801 on hearing impairment in the STZ-induced diabetic model, demonstrated by reducing the deterioration produced by DM in ABR threshold in response to clicks and normalizing wave I–IV latencies and Pa latencies in AMLR. We also show evidence that these effects might be elicited by inducing NGF related through Nr3c1 and Akt. Therefore, this result suggests that the neuroprotective effects of DA9801 on the auditory damage produced by DM may be affected by NGF increase resulting from Nr3c1 via Akt transformation. PMID:25878713

  19. Amelioration of Auditory Response by DA9801 in Diabetic Mouse.

    PubMed

    Lee, Yeong Ro; Hong, Bin Na; Her, You Ri; Castañeda, Rodrigo; Moon, Hyo Won; Kang, Tong Ho

    2015-01-01

    Diabetes mellitus (DM) is a metabolic disease that involves disorders such as diabetic retinopathy, diabetic neuropathy, and diabetic hearing loss. Recently, neurotrophin has become a treatment target that has shown to be an attractive alternative in recovering auditory function altered by DM. The aim of this study was to evaluate the effect of DA9801, a mixture of Dioscorea nipponica and Dioscorea japonica extracts, in the auditory function damage produced in a STZ-induced diabetic model and to provide evidence of the mechanisms involved in enhancing these protective effects. We found a potential application of DA9801 on hearing impairment in the STZ-induced diabetic model, demonstrated by reducing the deterioration produced by DM in ABR threshold in response to clicks and normalizing wave I-IV latencies and Pa latencies in AMLR. We also show evidence that these effects might be elicited by inducing NGF related through Nr3c1 and Akt. Therefore, this result suggests that the neuroprotective effects of DA9801 on the auditory damage produced by DM may be affected by NGF increase resulting from Nr3c1 via Akt transformation. PMID:25878713

  20. OpenDA-NEMO framework for ocean data assimilation

    NASA Astrophysics Data System (ADS)

    van Velzen, Nils; Altaf, Muhammad Umer; Verlaan, Martin

    2016-05-01

    Data assimilation methods provide a means to handle the modeling errors and uncertainties in sophisticated ocean models. In this study, we have created an OpenDA-NEMO framework unlocking the data assimilation tools available in OpenDA for use with NEMO models. This includes data assimilation methods, automatic parallelization, and a recently implemented automatic localization algorithm that removes spurious correlations in the model based on uncertainties in the computed Kalman gain matrix. We have set up a twin experiment where we assimilate sea surface height (SSH) satellite measurements. From the experiments, we can conclude that the OpenDA-NEMO framework performs as expected and that the automatic localization significantly improves the performance of the data assimilation algorithm by successfully removing spurious correlations. Based on these results, it looks promising to extend the framework with new kinds of observations and work on improving the computational speed of the automatic localization technique such that it becomes feasible to include large number of observations.

  1. Basic principles of pharmaceutical science in Ayurvĕda.

    PubMed

    Subhose, Varanasi; Srinivas, Pitta; Narayana, Ala

    2005-01-01

    Pharmaceutical is one of the allied branches of science, which is closely associated with Medical science. Today pharmaceutical chemistry and pharmacognosy are playing important role in treatment for a disease and its prevention. Herbal medicines are being used by about 80% of the world population mostly in the developing countries in the primary health care. There has been an upsurge in demand for the Phyto-pharmaceutical products of Ayurvĕda in western nations, because of the fact that the synthetic drugs are considered to be unsafe. Due to this many national and multinational pharmaceutical companies are now concentrating on manufacturing of Ayurvĕdic Phyto-pharmaceutical products. Ayurvĕda is the Indian traditional system of medicine, which also deals about pharmaceutical science. The Ayurvĕdic knowledge of the pharmaceutical science is scattered in Ayurvĕdic classical texts. Săranghadhara Samhita, which is written by Săranghadhara, explain systematically about the information of the Ayurvĕdic pharmaceutical science and also updated it. Industrialized manufacturing of Ayurvĕdic dosage forms has brought in new challenges like deviation from basic concepts of medicine preparation. Săranghadhara Samrhită the devout text on pharmaceutics in Ayurvĕda comes handy to solve such problems, as the methods described are very lucid and easy to follow. PMID:17333665

  2. Seeking 'energy' vs. pain relief in spas in Brazil (Caldas da Imperatriz) and Portugal (Termas da Sulfurea).

    PubMed

    Quintela, Maria Manuel

    2011-04-01

    This paper is a comparative ethnography of the therapeutic practices at two different spa locations: Caldas da Imperatriz, SC, Brazil, and Termas da Sulfurea in Cabeco de Vide, Portugal. The comparison reveals the existence of contrasting 'explanatory models' held by the spa-goers as well as by the official medical systems. In the Portuguese context this model is highly medicalized; in the Brazilian case, spa treatments are viewed as 'alternative' or 'complementary' therapy and are also related to religious philosophies. Each model corresponds to a different idiom expressing certain experiences and world views, one focusing on 'pains' (dores) and the other on 'energy' (energia), the former leading to the rationale of 'curing', the latter to the notion of 'energizing'. In this paper the author intends to analyze and contrast the categories found in these models, which originate from different conceptions of health, illness and healing for Brazilian and Portuguese spa-goers. PMID:21563001

  3. [The Arabic influence in the "Colóquios dos simples e drogas da India" of Garcia da Orta].

    PubMed

    Ricordel, Joëlle

    2015-09-01

    The "Colóquios dos simples e drogas he cousas medicinais de Índia" (Conversations on the simples, drugs and medicinal substances of India) (1563) of Garcia da Orta is a botanical and pharmacognosy book. The author is a Portuguese physician who studied in the Spanish universities and practiced medicine mainly in India. He studies in short chapters presented in the form of dialogues about sixty simples. Sources to which he refers are indicative of a "classical" training, but also the mark of a curious and open mind to different cultures. The Arabic sources are numerous and mainly concern the identification of substances by abundant synonyms of their names in foreign languages and different medicinal uses that may have been done by the ancient physicians. However, Da Orta is critical with respect to these sources, seeking contradictions and differences of opinion among authors. He confronts them with the oral information collected thanks to a wide network of contacts. PMID:26529894

  4. The 29-kDa proteins phosphorylated ion thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein

    SciTech Connect

    Mendelsohn, M.E.; Yan Zhu; O'Neill, S. )

    1991-12-15

    Thrombin plays a critical role in platelet activation, hemostasis, and thrombosis. Cellular activation by thrombin leads to the phosphorylation of multiple proteins, most of which are unidentified. The authors have characterized several 29-kDa proteins that are rapidly phosphorylated following exposure of intact human platelets to thrombin. A murine monoclonal antibody raised to an unidentified estrogen receptor-related 29-kDa protein selectively recognized these proteins as well as a more basic, unphosphorylated 27-kDa protein. Cellular activation by thrombin led to a marked shift in the proportion of protein from the 27-kDa unphosphorylated form to the 29-kDa phosphoprotein species. Using this antibody, they isolated and sequenced a human cDNA clone encoding a protein that was identical to the mammalian 27-kDa heat shock protein (HSP27), a protein of uncertain function that is known to be phosphorylated to several forms and to be transcriptionally induced by estrogen. The 29-kDa proteins were confirmed to be phosphorylated forms of HSP27 by immunoprecipitation studies. Thus, the estrogen receptor-related protein is HSP27, and the three major 20-kDa proteins phosphorylated in thrombin-activated platelets are forms of HSP27. These data suggest a role for HSP27 in the signal transduction events of platelet activation.

  5. Identification of sequence similarity between 60 kDa and 70 kDa molecular chaperones: evidence for a common evolutionary background?

    PubMed Central

    Flores, A I; Cuezva, J M

    1997-01-01

    Recent findings support the premise that chaperonins (60 kDa stress-proteins) and alpha-subunits of F-type ATPases (alpha-ATPase) are evolutionary related protein families. Two-dimensional gel patterns of synthesized proteins in unstressed and heat-shocked embryonic Drosophila melanogaster SL2 cells revealed that antibodies raised against the alpha-subunit of the F1-ATPase complex from rat liver recognize an inducible p71 member of the 70 kDa stress-responsive protein family. Molecular recognition of this stress-responsive 70 kDa protein by antibodies raised against the F1-ATPase alpha-subunit suggests the possibility of partial sequence similarity within these ATP-binding protein families. A multiple sequence alignment between alpha-ATPases and 60 kDa and 70 kDa molecular chaperones is presented. Statistical evaluation of sequence similarity reveals a significant degree of sequence conservation within the three protein families. The finding suggests a common evolutionary origin for the ATPases and molecular chaperone protein families of 60 kDa and 70 kDa, despite the lack of obvious structural resemblance between them. PMID:9065788

  6. Da Vinci's codex and the anatomy of healthcare.

    PubMed

    Stephens-Borg, Keith

    2012-08-01

    We usually display a laid-back approach to medical jargon throughout our theatre work. The word 'perioperative' is built from the Greek word 'peri' (around) and the Latin 'operari' (to work). Latin and Greek became the prefixed language of choice for Leonardo da Vinci, and his research was pivotal in determining the way in which surgical procedures are documented. Ancient manuscripts aided the unfolding of the secrets of anatomy, and Leonardo revealed that art was the key in expressive detailed explanation. PMID:23248927

  7. Stereoscopic augmented reality for da Vincii™ robotic biliary surgery

    PubMed Central

    Volonté, Francesco; Buchs, Nicolas C.; Pugin, François; Spaltenstein, Joël; Jung, Minoa; Ratib, Osman; Morel, Philippe

    2013-01-01

    INTRODUCTION New laparoscopic techniques put distance between the surgeon and his patient. PRESENTATION OF CASE 3D volume rendered images directly displayed in the da Vinci surgeon's console fill this gap by allowing the surgeon to fully immerse in its intervention. DISCUSSION During the robotic operation the surgeon has a greater control on the procedure because he can stay more focused not being obliged to turn is sight out of his operative field. Moreover, thanks to depth perception of the rendered images he had a precise view of important anatomical structures. CONCLUSION We describe our preliminary experience in the quest of computer-assisted robotic surgery. PMID:23466685

  8. Ultratight crystal packing of a 10 kDa protein

    SciTech Connect

    Trillo-Muyo, Sergio; Chruszcz, Maksymilian; Minor, Wladek; Kuisiene, Nomeda

    2013-03-01

    The crystal structure of the C-terminal domain of a putative U32 peptidase from G. thermoleovorans is reported; it is one of the most tightly packed protein structures reported to date. While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.

  9. A quasi-passive CMOS pipeline D/A converter

    NASA Technical Reports Server (NTRS)

    Wang, Fong-Jim; Temes, Gabor C.; Law, Simon

    1989-01-01

    A novel pipeline digital-to-analog converter configuration, based on switched-capacitor techniques, is described. An n-bit D/A conversion can be implemented by cascading n + 1 unit cells. The device count of the circuit increases linearly, not exponentially, with the conversion accuracy. The new configuration can be pipelined. Hence, the conversion rate can be increased without requiring a higher clock rate. An experimental 10-bit DAC prototype has been fabricated using a 3-micron CMOS process. The results show that high-speed, high-accuracy, and low-power operation can be achieved without special process or postprocess trimming.

  10. Leonardo da Vinci and the origin of semen

    PubMed Central

    Noble, Denis; DiFrancesco, Dario; Zancani, Diego

    2014-01-01

    It is well known that Leonardo da Vinci made several drawings of the human male anatomy. The early drawings (before 1500) were incorrect in identifying the origin of semen, where he followed accepted teaching of his time. It is widely thought that he did not correct this mistake, a view that is reflected in several biographies. In fact, he made a later drawing (after 1500) in which the description of the anatomy is remarkably accurate and must have been based on careful dissection. In addition to highlighting this fact, acknowledged previously in only one other source, this article reviews the background to Leonardo's knowledge of the relevant anatomy. PMID:27494016

  11. Visual tracking of da Vinci instruments for laparoscopic surgery

    NASA Astrophysics Data System (ADS)

    Speidel, S.; Kuhn, E.; Bodenstedt, S.; Röhl, S.; Kenngott, H.; Müller-Stich, B.; Dillmann, R.

    2014-03-01

    Intraoperative tracking of laparoscopic instruments is a prerequisite to realize further assistance functions. Since endoscopic images are always available, this sensor input can be used to localize the instruments without special devices or robot kinematics. In this paper, we present an image-based markerless 3D tracking of different da Vinci instruments in near real-time without an explicit model. The method is based on different visual cues to segment the instrument tip, calculates a tip point and uses a multiple object particle filter for tracking. The accuracy and robustness is evaluated with in vivo data.

  12. Effects of DA-Phen, a dopamine-aminoacidic conjugate, on alcohol intake and forced abstinence.

    PubMed

    Sutera, Flavia Maria; De Caro, Viviana; Cannizzaro, Carla; Giannola, Libero Italo; Lavanco, Gianluca; Plescia, Fulvio

    2016-09-01

    The mesolimbic dopamine (DA) system plays a key role in drug reinforcement and is involved in the development of alcohol addiction. Manipulation of the DAergic system represents a promising strategy to control drug-seeking behavior. Previous studies on 2-amino-N-[2-(3,4-dihydroxy-phenyl)-ethyl]-3-phenyl-propionamide (DA-Phen) showed in vivo effects as a DA-ergic modulator. This study was aimed at investigate DA-Phen effects on operant behavior for alcohol seeking behavior, during reinstatement following subsequent periods of alcohol deprivation. For this purpose, male Wistar rats were tested in an operant paradigm of self-administration; behavioral reactivity and anxiety like-behavior during acute abstinence were evaluated. A characterization of DA-Phen CNS targeting by its quantification in the brain was also carried out. Our findings showed that DA-Phen administration was able to reduce relapse in alcohol drinking by 50% and reversed the alterations in behavioral reactivity and emotionality observed during acute abstinence. In conclusion, DA-Phen can reduce reinstatement of alcohol drinking in an operant-drinking paradigm following deprivation periods and reverse abstinence-induced behavioral phenotype. DA-Phen activity seems to be mediated by the modulation of the DAergic transmission. However further studies are needed to characterize DA-Phen pharmacodynamic and pharmacokinetic properties, and its potential therapeutic profile in alcohol addiction. PMID:27155501

  13. Evolution of the ATLAS PanDA workload management system for exascale computational science

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Klimentov, A.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.; Yu, D.; Atlas Collaboration

    2014-06-01

    An important foundation underlying the impressive success of data processing and analysis in the ATLAS experiment [1] at the LHC [2] is the Production and Distributed Analysis (PanDA) workload management system [3]. PanDA was designed specifically for ATLAS and proved to be highly successful in meeting all the distributed computing needs of the experiment. However, the core design of PanDA is not experiment specific. The PanDA workload management system is capable of meeting the needs of other data intensive scientific applications. Alpha-Magnetic Spectrometer [4], an astro-particle experiment on the International Space Station, and the Compact Muon Solenoid [5], an LHC experiment, have successfully evaluated PanDA and are pursuing its adoption. In this paper, a description of the new program of work to develop a generic version of PanDA will be given, as well as the progress in extending PanDA's capabilities to support supercomputers and clouds and to leverage intelligent networking. PanDA has demonstrated at a very large scale the value of automated dynamic brokering of diverse workloads across distributed computing resources. The next generation of PanDA will allow other data-intensive sciences and a wider exascale community employing a variety of computing platforms to benefit from ATLAS' experience and proven tools.

  14. [Textual Research on Chiding Disease and Matixiang Herb in Da he wai ke (External Medicine of Da He)].

    PubMed

    Pu, Xiao-Lan; Zhang, Yi; Yang, Wen-Yu; Wu, Ya-Mei

    2012-11-01

    Recorded in Da he wai ke (The External Medicine of Da He) in the Ming dynasty without a corresponding disease title in contemporary scholarly concepts of Traditional Chinese Medicine (TCM), Chiding disease was described as an acute symptomatic disease with inevitable serious conditions of convulsion-like or mania-like behavior, and as a refractory ailment. It was also considered as an alias of Hongsiding (namely the acute lymphangitis) in few ancient books. Matixiang, a Chinese herb for treatment of Chiding, was regarded as a nonsuch drug by Da he wai ke, and was considered as a take-then-cure drug by Chuang yang jing yan quan shu (Compendium for Experience in Sores Diseases). The present studies about Chiding and Matixiang show that: (1) the name of Chiding was first seen in Zhong zang jing (Master Hua's Classic of the Viscera) ; (2) Chiding was rarely mentioned in medical literature of the Song, Yuan and Qing Dynasty, but mostly seen in the Ming Dynasty; (3) Xinding and Huoyanding were found as synonyms of Chiding ; (4) the distinction between Chiding and Hongsiding (flaming sore) was revealed by comparison analysis of their etiology, affected part(s), symptoms, prognosis and syndromic pictures; (5) Chiding on the tongue is the same as "Tongue Ding" or "Sublingual Ding" in contemporary TCM and as "Sublingual Space Infection" in Western Medicine. Moreover, Chiding on the hands, feet or other skin parts could be classified as "Hands/Feet Ding" in contemporary TCM and as "Furuncle" (i.e., Acute Suppurative Folliculitis and Perifolliculitis or cellulites of the tongue) in Western Medicine; (6) Matixiang is probably deduced as Asarum forbesii Maxim., A. ichangense C. Y. Cheng et C. S. Yang, family Aristolochiaceae, or Valeriana jatamansi Jones., family Valerianaceae. PMID:23363849

  15. Chandra Confirmation of a Pulsar Wind Nebula in DA 495

    NASA Technical Reports Server (NTRS)

    Arzoumanian, Z.; Safi-Harb, S.; Landecker, T.L.; Kothes, R.; Camilo, F.

    2008-01-01

    As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected X-ray source at the heart of the annular radio nebula, establishing the radiative properties of two key emission components: a soft unresolved source with a blackbody temperature of 1 MK consistent with a neutron star, surrounded by a nontherma1 nebula 40" in diameter exhibiting a power-law spectrum with photon index Gamma = 1.63, typical of a pulsar wind nebula. Morphologically, the nebula appears to be slightly extended along a direction, in projection on the sky, previously demonstrated to be of significance in radio and ASCA observations; we argue that this represents the orientation of the pulsar spin axis. At smaller scales, a narrow X-ray feature is seen extending out 5" from the point source, but energetic arguments suggest that it is not the resolved termination shock of the pulsar wind against the ambient medium. Finally, we argue based on synchrotron lifetimes in the nebular magnetic field that DA 495 represents the first example of a pulsar wind nebula in which electromagnetic flux makes up a significant part, together with particle flux, of the neutron star's wind.

  16. The DA+dMe Binary WD 0334-6400

    NASA Astrophysics Data System (ADS)

    Barrett, P. E.; Schlegel, E. M.; Dupuis, J.; Heathcote, B.

    2005-12-01

    Multiwavelength observations of the star WD 0334-6400 (or Ret 1 in A Catalog and Altas of Cataclysmic Variables: Living Edition) show it to be a DA+dMe binary. Our analysis of the far-ultraviolet continuum and spectral lines reveal that Teff = 36500 K; log g = 7.7; and the photosphere contains C, N, O, Si, P, and S. C/H = 2 x 10-6, which is the highest abundance seen in a DA white dwarf at this temperature. The dMe star is seen as a flux excess in near-infrared photometry and appears to show occasional flaring of about one magnitude as seen in the Harvard plates. There is no evidence of periodic variability in the spectroscopic or photometric data. This paper is based on observations made with the NASA-CNES-CSA FUSE. This research is partially supported by NASA FUSE grants NAG5-12413 and NNG04GL18G.

  17. The 37kDa/67kDa Laminin Receptor acts as a receptor for Aβ42 internalization

    PubMed Central

    Da Costa Dias, Bianca; Jovanovic, Katarina; Gonsalves, Danielle; Moodley, Kiashanee; Reusch, Uwe; Knackmuss, Stefan; Weinberg, Marc S.; Little, Melvyn; Weiss, Stefan F. T.

    2014-01-01

    Neuronal loss is a major neuropathological hallmark of Alzheimer's disease (AD). The associations between soluble Aβ oligomers and cellular components cause this neurotoxicity. The 37 kDa/67 kDa laminin receptor (LRP/LR) has recently been implicated in Aβ pathogenesis. In this study the mechanism underlying the pathological role of LRP/LR was elucidated. Försters Resonance Energy Transfer (FRET) revealed that LRP/LR and Aβ form a biologically relevant interaction. The ability of LRP/LR to form stable associations with endogenously shed Aβ was confirmed by pull down assays and Aβ-ELISAs. Antibody blockade of this association significantly lowered Aβ42 induced apoptosis. Furthermore, antibody blockade and shRNA mediated downregulation of LRP/LR significantly hampered Aβ42 internalization. These results suggest that LRP/LR is a receptor for Aβ42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide by facilitating intra-cellular Aβ42 accumulation. These findings recommend anti-LRP/LR specific antibodies and shRNAs as potential therapeutic tools for AD treatment. PMID:24990253

  18. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form...

  19. A Value-Engaged Approach for Evaluating the Bunche-Da Vinci Learning Academy

    ERIC Educational Resources Information Center

    Greene, Jennifer C.

    2005-01-01

    In 2001, the Bunche Academy was chosen by its district to join in partnership with the Da Vinci Learning Corporation to embark on an ambitious whole-school reform initiative, especially designed by the corporation for low-performing schools. In this chapter, the author describes how, as illustrated in the Bunche-Da Vinci Learning Academy context,…

  20. Processing and Visualizing Planetary Data Using DaVinci: Updates for Portability and Scriptable Execution

    NASA Astrophysics Data System (ADS)

    Edwards, C. S.; Anwar, S.; Hagee, W.; Doerres, D.; Dickensheid, S.; Christensen, P. R.

    2015-06-01

    We present updates to the DaVinci software toolkit that enable stand-alone reading of ISIS3 files, a link between DaVinci and JMARS, the ingestion and use of geospatial information, and a series of executable THEMIS processing scripts.

  1. The Indicative and Subjunctive "da"-complements in Serbian A Syntactic-Semantic Approach

    ERIC Educational Resources Information Center

    Todorovic, Natasa

    2012-01-01

    A syntactic-semantic investigation of subjunctive and indicative "da"-complements in Serbian is conducted in this project. After a careful comparison of Serbian sentence constructions with "da"-complements to the equivalent sentence structures in languages of the Balkans as well as other Slavic languages, it is clearly…

  2. Paśu Ayurvĕda (veterinary medicine) in Garudapurăņa.

    PubMed

    Varanasi, Subhose; Narayana, A

    2007-01-01

    The history of veterinary medicine is closely tied to the development of human medicine. Evidence of animal medicine has been found in ancient civilizations, such as those of the Hindu, Babylonians, Hebrews, Arabs, Greeks, and Romans. Ancient Indian literature in the form of the holy Vĕda, Purăna, Brăhmaņa, epics, etc. is flooded with information on animal care. The Purăņa are ancient scriptures discuss varied topics like devotion to God and his various aspects, traditional sciences like Ayurvĕda, Jyŏtişa (Astrology), cosmology, concepts like dharma, karma, reincarnation and many others. The treatment of animal diseases using Ayurvedic medicine has been mentioned in Garudapurăna, Agnipurăņa, Atri-samhită, Matsyapurăņa and many other texts. The Garudapurăņa is one of the important Săttvika purăna, the subject matter is divided into two parts, viz. Pŭrvakhaņda (first part) and an Uttarakhaņda (subsequent part). Gavăyurvĕda, Gajăyurvĕda narrated briefly and Aśvăyurvĕda described detailly in Pŭrvakhaņda. PMID:19580108

  3. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form...

  4. Topological studies of spinach 22 kDa protein of Photosystem II.

    PubMed

    Kim, S; Pichersky, E; Yocum, C F

    1994-12-30

    An intrinsic 22 kDa polypeptide is associated with the O2-evolving Photosystem II core complex in a variety of green plants, although it does not appear to be required for O2 evolution. Digestion of thylakoid membranes and isolated Photosystem II preparations with trypsin, followed by immunoblotting using spinach anti-22 kDa antibodies, leads to two observations: (1) the domain between the 2nd and 3rd transmembrane helices of the 22 kDa protein is stromally exposed, and (2) only in a reaction center complex preparation, lacking the chlorophyll a/b-light harvesting complex II, is there extensive proteolytic cleavage of the 22 kDa protein. We also found that after, but not prior to, selective extraction of the 22 and 10 kDa proteins from Photosystem II membranes, the chlorophyll a/b-light harvesting complex II can be separated from the Photosystem II reaction center core by precipitation with MgCl2. This result suggests that the 22 kDa polypeptide is located between the Photosystem II reaction center polypeptides and light-harvesting complex II; it is possible that the protein serves as a link between the two protein complexes. The presence of the 22 kDa protein in several species was also examined by immunoblotting with polyclonal spinach anti-22 kDa antibodies. PMID:7803450

  5. 32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form...

  6. A Creative Approach to the Common Core Standards: The Da Vinci Curriculum

    ERIC Educational Resources Information Center

    Chaucer, Harry

    2012-01-01

    "A Creative Approach to the Common Core Standards: The Da Vinci Curriculum" challenges educators to design programs that boldly embrace the Common Core State Standards by imaginatively drawing from the genius of great men and women such as Leonardo da Vinci. A central figure in the High Renaissance, Leonardo made extraordinary contributions as a…

  7. Pharmacological action of DA-9701 on the motility of feline stomach circular smooth muscle.

    PubMed

    Nguyen, Thanh Thao; Song, Hyun Ju; Ko, Sung Kwon; Sohn, Uy Dong

    2015-03-01

    DA-9701, a new prokinetic agent for the treatment of functional dyspepsia, is formulated with Pharbitis semen and Corydalis tuber. This study wasconducted to determine the pharmacological action of DA-9701 and to identify the receptors involved in DA-9701 -induced contractile responsesin the feline gastric corporal, fundic and antral circular smooth muscle. Concentration-response curve to DA-9701 was established. The tissue trips were exposed to methylsergide, ketanserin, ondansetron, GR 113808, atropine and dopamine before administration of DA-9701. The contractile force was determined before and after administration of drugs by a polygraph.DA-9701 enhanced the spontaneous contractile amplitude of antrum, corpus and fundus. However, it did not change the spontaneous contractile frequency of antrum and corpus, but concentration-dependently reduced that of fundus. In the fundus, DA-9701 -induced tonic contractions were inhibited by dopamine, methylsergide, ketanserine, ondansetron or GR 113808 respectively, but not by atropine, indicating that the contractile responses are mediated by multiple receptors: 5-HT2, 5-HT3, 5-HT4, and dopamine receptors. In the corpus, DA-9701-induced contractions were blocked by atropine, dopamine or GR 113808, but not by methysergide, ketanserin or ondansetron, indicating that they are involved in receptors on both, smooth muscles and neurons: 5-HT4 and dopamine receptors. However, contractile responses to DA-9701 are mainly mediated by dopamine receptors in the antrum. These results suggest that DA-9701 has important roles in gastric accommodation by enhancing tonic activity of fundus, and in gastric emptying and gastrointestinal transit by phasic contractions of corpus and antrum mediated by multiple receptors. PMID:25980179

  8. The Effect of DA-9701 in Opioid-induced Bowel Dysfunction of Guinea Pig

    PubMed Central

    Hussain, Zahid; Rhee, Kwang Won; Lee, Young Ju; Park, Hyojin

    2016-01-01

    Background/Aims Opioid induced bowel dysfunction (OIBD) is associated with decreased gastrointestinal (GI) propulsive activity due to intake of opioid analgesics. DA-9701, a novel prokinetic agent formulated with Pharbitis Semen and Corydalis Tuber has promising effects on GI motor function. Therefore, we aim to evaluate the prokinetic effects of DA-9701 in an OIBD model of guinea pig. Methods The ileal and distal colon muscle contraction in presence of different doses of DA-9701, morphine, and combination (morphine + DA-9701) was measured by tissue bath study. The prokinetic effect of DA-9701 was assessed by charcoal transit and fecal pellet output assay in an OIBD model of guinea pig. Results DA-9701 significantly increased the amplitude and area under the curve of ileal muscle contraction, while there was insignificant effect on the distal colon compared to the control. The maximal amplitude of ileal muscle contraction was acquired at a concentration of 10 μg/mL of DA-9701. In contrast, morphine significantly decreased the amplitude of ileal and distal colon muscle contraction compared to the control. Morphine delayed both upper (P < 0.01) and lower (P < 0.05) GI transit, and delayed GI transit was restored by the administration of DA-9701. Morphine induced reduction of contractility was significantly ameliorated by addition of DA-9701 in both ileal and distal colon muscles. Conclusions DA-9701 significantly increased the amplitude of contraction of the ileal muscle, however the distal colon muscle contraction was insignificant. Additionally, it restored delayed upper and lower GI transit in an OIBD model of guinea pig, and it might prove to be a useful candidate drug in a clinical trial for OIBD. PMID:26932898

  9. [Regarding the Manuscript D " Dell' occhio " of Leonardo da Vinci].

    PubMed

    Heitz, Robert F

    2009-01-01

    Leonardo da Vinci's Manuscript D consists of five double pages sheets, which, folded in two, comprise ten folios. This document, in the old Tuscan dialect and mirror writing, reveals the ideas of Leonardo on the anatomy of the eye in relation to the formation of images and visual perception. Leonardo explains in particular the behavior of the rays in the eye in terms of refraction and reflection, and is very mechanistic in his conception of the eye and of the visual process. The most significant innovations found in these folios are the concept of the eye as a camera obscura and the intersection of light rays in the interior of the eye. His texts nevertheless show hesitation, doubts and a troubled confusion, reflecting the ideas and uncertainties of his era. He did not share his results in his lifetime, despite both printing and etching being readily available to him. PMID:19852385

  10. A 92-kDa human immunostimulatory protein.

    PubMed Central

    Fontan, E; Briend, E; Saklani-Jusforgues, H; d'Alayer, J; Vandekerckhove, J; Fauve, R M

    1994-01-01

    We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 micrograms/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety. Images PMID:8078887

  11. DBS -- An rlogin multiplexer and output logger for DA systems

    SciTech Connect

    Oleynik, G.; Appleton, L.; Udumula, L.; Votava, M.

    1994-04-01

    DART Bootstrap Services (dbs) is the first component of run-control for the DART Data Acquisition system -- the DA for the 96` round of experiments at Fermilab -- though it has potential usefulness as a powerful tool in other distributed applications. dbs is an rlogin session multiplexer. It allows a user, running a single program, to start up any number of remote login sessions, feed shell commands to them, and collect their output into a single (or multiple) log files (a server keeps the sessions open and collects their output). From this program, any session can be attached to interactively so it appears just like an rlogin session -- dbs becomes transparent. When finished with this interactive mode, the user can escape back to dbs and attach to a different session if so desired. Among many other useful features, dbs supplies a mechanism for cleanup (deletion) of all processes created under a session, allowing a fresh start.

  12. VLBI Radar of the 2012 DA14 Asteroid

    NASA Astrophysics Data System (ADS)

    Nechaeva, M. B.; Dugin, N. A.; Antipenko, A. A.; Bezrukov, D. A.; Bezrukov, V. V.; Voytyuk, V. V.; Dement'ev, A. F.; Jekabsons, N.; Klapers, M.; Konovalenko, A. A.; Kulishenko, V. F.; Nabatov, A. S.; Nesteruk, V. N.; Putillo, D.; Reznichenko, A. M.; Salerno, E.; Snegirev, S. D.; Tikhomirov, Yu. V.; Khutornoy, R. V.; Skirmante, K.; Shmeld, I.; Chagunin, A. K.

    2015-03-01

    An experiment on VLBI radar of the 2012 DA14 asteroid was carried out on February 15-16, 2011 at the time of its closest approach to the Earth. The research teams of Kharkov (Institute of Radio Astronomy of the National Academy of Sciences of Ukraine), Evpatoria (National Space Facilities Control and Test Center), Nizhny Novgorod (Radiophysical Research Institute), Bologna (Istituto di Radioastronomia (INAF)), and Ventspils (Ventspils International Radioastronomy Center) took part in the experiment. The asteroid was irradiated by the RT-70 planetary radar (Evpatoria) at a frequency of 5 GHz. The reflected signal was received using two 32-m radio telescopes in Medicina (Italy) and Irbene (Latvia) in radiointerferometric mode. The Doppler frequency shifts in bi-static radar mode and interference frequency in VLBI mode were measured. Accuracy of the VLBI radar method for determining the radial and angular velocities of the asteroid were estimated.

  13. 70 DA WHITE DWARFS IDENTIFIED IN LAMOST PILOT SURVEY

    SciTech Connect

    Zhao, J. K.; Luo, A. L.; Zhao, G.; Oswalt, T. D. E-mail: gzhao@bao.ac.cn E-mail: toswalt@fit.edu

    2013-06-01

    We present a spectroscopically identified catalog of 70 DA white dwarfs (WDs) from the LAMOST pilot survey. Thirty-five are found to be new identifications after cross-correlation with the Eisenstein et al. and Villanova catalogs. The effective temperature and gravity of these WDs are estimated by Balmer lines fitting. Most of them are hot WDs. The cooling times and masses of these WDs are estimated by interpolation in theoretical evolution tracks. The peak of the mass distribution is found to be {approx}0.6 M {sub Sun }, which is consistent with prior work in the literature. The distances of these WDs are estimated using the method of synthetic spectral distances. All of these WDs are found to be in the Galactic disk from our analysis of space motions. Our sample supports the expectation that WDs with high mass are concentrated near the plane of the Galactic disk.

  14. DBS - an rlogin multiplexor and output logger for DA systems

    SciTech Connect

    Oleynik, G.; Appleton, L.; Udumula, L.; Votava, M.

    1994-12-31

    DART Bootstrap Services (dbs) is the first component of run-control for the DART Data Acquisition system - the DA for the 96{prime} round of experiments at Fermilab - though it has potential usefulness as a powerful tool in other distributed applications, dbs is an rlogin session multiplexer. It allows a user, running a single program, to start up any number of remote login sessions, feed shell commands to them, and collect their output into a single (or multiple) logfiles (a server keeps the sessions open and collects their output). From this program, any session can be attached to interactively so it appears just like an rlogin session - dbs becomes transparent. When finished with this interactive mode, the user can escape back to dbs and attach to a different session if so desired. Among many other useful features, dbs supplies a mechanism for cleanup (deletion) of all processes created under a session, allowing a fresh start.

  15. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... SOURCES Standards of Performance for Electric Utility Steam Generating Units § 60.45Da Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  16. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... SOURCES Standards of Performance for Electric Utility Steam Generating Units § 60.45Da Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  17. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... SOURCES Standards of Performance for Electric Utility Steam Generating Units § 60.45Da Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  18. Antimicrobial Properties of an Immunomodulator - 15 kDa Human Granulysin

    PubMed Central

    Wei, Hung-Mu; Lin, Li-Chih; Wang, Chiu-Feng; Lee, Yi-Jang; Chen, Yuan-Tsong; Liao, You-Di

    2016-01-01

    Granulysin, a cationic protein expressed by human natural killer cells and cytotoxic T lymphocytes, is a mediator for drug-induced Stevens-Johnson syndrome and graft-versus-host disease. Some 15 kDa granulysin are processed into 9 kDa forms and sequestered in cytolytic granules, while others are constitutively secreted into body fluids. Both 9 and 15 kDa granulysin have been shown to be a serum marker for cell-mediated immunity. Furthermore, 15 kDa is able to activate monocyte differentiation. However, its antimicrobial properties have not been clearly addressed. Here, we report a novel method to prepare both the soluble 9 and 15 kDa granulysin and show that the 15 kDa form is more effective than the 9 kDa form in exerting specific antimicrobial activity against Pseudomonas aeruginosa within a range of few micromolars. We also show that the 15 kDa granulysin is able to hyperpolarize the membrane potential and increase membrane permeability of treated bacteria. Interestingly, the bactericidal activity and membrane permeability of the granulysins were markedly reduced at lower pH (pH 5.4) as a result of probable increase in hydrophobicity of the granulysins. Additionally, we’ve also shown the granulysin to inhibit biofilm formation by P. aeruginosa. These results suggest that the 15 kDa granulysin exhibits a novel mechanism in bacteria killing in a way that’s different from most antimicrobial peptides. Our novel granulysin preparation methodology will be useful for further study of action mechanisms of other antimicrobial, cytotoxic and immunomodulating properties in granulysin-mediated diseases. PMID:27276051

  19. Neospora caninum: identification of 19-, 38-, and 40-kDa surface antigens and a 33-kDa dense granule antigen using monoclonal antibodies.

    PubMed

    Schares, G; Dubremetz, J F; Dubey, J P; Bärwald, A; Loyens, A; Conraths, F J

    1999-06-01

    Neospora caninum, a coccidian parasite closely related to Toxoplasma gondii, can infect a broad host range and is regarded as an important cause of bovine abortion worldwide. In the present study, four antigens of N. caninum were partially characterized using monoclonal antibodies. Immunofluorescence of viable tachyzoites as well as the immunoprecipitation of antigens extracted from tachyzoites previously labeled by surface biotinylation revealed that three of these antigens with apparent molecular weights of 40, 38, and 19 kDa are located in the outer surface membrane of this parasite stage. Further evidence for the surface localization of the 38-kDa antigen was obtained by immunoelectron microscopy. In addition to the surface molecules, an antigen located in dense granules and in the tubular network of the parasitophorous vacuole was detected by another monoclonal antibody. When tachyzoite antigens separated under nonreducing conditions were probed on Western blots, this antibody reacted mainly with a 33-kDa antigen. Immunohistochemical analysis of infected tissue sections indicated that the 33-kDa dense granule antigen is present in both tachyzoites and bradyzoites, while the 38-kDa surface antigen from tachyzoites seems to be absent in bradyzoites. PMID:10366536

  20. Respostas religiosas à aids no Brasil: impressões de pesquisa acerca da Pastoral de DST/Aids da Igreja Católica1

    PubMed Central

    Seffner, Fernando; Silva, Cristiane Gonçalves Meireles da; Maksud, Ívia; Garcia, Jonathan; Rios, Luís Felipe; Natividade, Marcelo; Borges, Priscila Rodrigues; Parker, Richard; Terto, Veriano

    2009-01-01

    O texto encontra-se estruturado em quatro partes. Na primeira delas, apresentamos um conjunto de considerações e informações acerca da situação da aids no Brasil, das relações entre religião, sexualidade, aids e estado laico, bem como uma descrição mais clara do Projeto Respostas Religiosas ao HIV/Aids no Brasil, do qual este texto apresenta algumas impressões de pesquisa preliminares. A seguir, dedicamos um item a apresentação da Pastoral de DST/Aids, sua história, estrutura e objetivos. No item seguinte problematizamos diversas questões em particular no âmbito das relações Estado e Igreja, relações entre agentes de pastoral e hierarquia da Igreja, e questões ligadas mais diretamente à sexualidade e aids, todas referenciadas ao trabalho da Pastoral de DST/Aids. Ao final, apresentamos a bibliografia e fontes consultadas. PMID:20442806

  1. PD2P: PanDA Dynamic Data Placement for ATLAS

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Panitkin, S.

    2012-12-01

    The PanDA (Production and Distributed Analysis) system plays a key role in the ATLAS distributed computing infrastructure. PanDA is the ATLAS workload management system for processing all Monte-Carlo (MC) simulation and data reprocessing jobs in addition to user and group analysis jobs. The PanDA Dynamic Data Placement (PD2P) system has been developed to cope with difficulties of data placement for ATLAS. We will describe the design of the new system, its performance during the past year of data taking, dramatic improvements it has brought about in the efficient use of storage and processing resources, and plans for the future.

  2. Stability of new carbapenem DA-1131 to renal dipeptidase (dehydropeptidase I).

    PubMed

    Park, Sung Wook; We, Jeoung Soon; Kim, Gye Won; Choi, Seong Hak; Park, Haeng Soon

    2002-02-01

    The stability of DA-1131 to renal dipeptidase (RDPase) (EC 3.4.13.19) was compared with that of imipenem and meropenem by V(max)/K(m) ratios as an index of the enzyme's preference for substrates. Our results showed a decreasing order of imipenem (6.24), meropenem (2.41), and DA-1131 (1.39). The biochemical evaluation of DA-1131 as the least preferred substrate of RDPase suggests its potential use as a novel beta-lactam antibiotic which may be usable without coadministration of RDPase inhibitors once its clinical suitability is proven. PMID:11796382

  3. Arbitrary Amplitude DIA and DA Solitary Waves in Adiabatic Dusty Plasmas

    SciTech Connect

    Mamun, A. A.; Jahan, N.; Shukla, P. K.

    2008-10-15

    The dust-ion-acoustic (DIA) as well as the dust-acoustic (DA) solitary waves (SWs) in an adiabatic dusty plasma are investigated by the pseudo-potential approach which is valid for arbitrary amplitude SWs. The role of the adiabaticity of electrons and ions in modifying the basic features (polarity, speed, amplitude and width) of arbitrary amplitude DIA and DA SWs are explicitly examined. It is found that the effects of the adiabaticity of electrons and ions significantly modify the basic features (polarity, speed, amplitude and width) of the DIA and DA SWs. The implications of our results in space and laboratory dusty plasmas are briefly discussed.

  4. 2012 DA14 and Chelyabinsk: Same Day Coincidence?

    NASA Astrophysics Data System (ADS)

    Chapman, Clark R.

    2013-10-01

    A long anticipated near-miss by near-Earth asteroid (NEA) 2012 DA14 on 15 February 2013 was upstaged 16 hours earlier by the impact and atmospheric explosion of a ~20 m NEA over Chelyabinsk, Russia. DA14 was earlier estimated to be 45 m across and passage at a distance under geosynchronous satellites was considered to be a record close approach by an object of that size, a once-in-40-years event. Actual Earth impact by a 20 m NEA had been estimated to be a once-in-200-years event. A simplistic calculation gives a probability of these happening on the same day of 1-in-a-billion. Within hours of the Chelyabinsk impact, it was recognized and widely reported that the two asteroids were in very different orbits and could not, at least in any readily understandable way, be physically related to each other (e.g. fragments of the same precursor body). Also, some early reports suggested that the two asteroids had very different compositions, further undermining the possibility they were pieces of the same body. (It seems unlikely, though certainly conceivable, that the two NEAs could have different compositions yet have some kind of causal connection resulting in the same-day events.) Nevertheless, incredibly tiny probabilities beg for an explanation. Here, with the benefit of hindsight, I re-examine issues that affect the probabilities, such as size, albedo, probable composition, and numbers of NEAs of these sizes. I also consider difficult issues of framing the apparent coincidence, which dominate other uncertainties. Updated information about the physical nature of the two asteroids somewhat modifies the original estimates of probabilities. Moreover, more proper ways of framing elements of the coincidence considerably reduce the improbability of the same-day events, but not nearly to the level (e.g. 1-in-1000 or perhaps 1-in-10,000) where we could rationalize dismissing the events as “just a coincidence.” The events of 15 February 2013 deserve more sophisticated

  5. D.A. Henderson: acting globally, thinking locally.

    PubMed

    Henderson, D A

    2003-08-01

    They had said that it couldn't be done-the worldwide eradication of smallpox. To hear D.A. Henderson tell it, the job of leading the World Health Organization's initiative to conquer the disease in the 1960s and 1970s rather fell into his lap. In fact, he describes each of the posts that he has held with great modesty, beginning with his military service at the Centers for Disease Control and Prevention all the way through his assignments as Dean of Public Health at Johns Hopkins, Associate Director of the Office of Science and Technology Policy in the Executive Office of the President, and more recently as Director of the Office of Public Health Preparedness under Secretary Thompson at the Department of Health and Human Services. Confronted with enormous challenges in terms of public health initiatives, Henderson describes each assignment as a matter of communicating with the people he works with and the people that he serves, and drawing on their insights to devise strategies for accomplishing the task at hand. With bioterrorism posing one of the major public health concerns to face the United States and the world, it's gratifying to know that someone with Henderson's track record and wide-ranging expertise is paying attention and making sure that medical and government officials are preparing to respond to the threat. Again and again, Henderson appears to have the knack for showing up in the right place at the right time with just the right idea. PMID:14993437

  6. Robotic Partial Nephrectomy with the Da Vinci Xi

    PubMed Central

    Kallingal, George J. S.; Swain, Sanjaya; Darwiche, Fadi; Punnen, Sanoj; Manoharan, Murugesan; Gonzalgo, Mark L.; Parekh, Dipen J.

    2016-01-01

    Purpose. The surgical expertise to perform robotic partial nephrectomy is heavily dependent on technology. The Da Vinci Xi (XI) is the latest robotic surgical platform with significant advancements compared to its predecessor. We describe our operative technique and experience with the XI system for robotic partial nephrectomy (RPN). Materials and Methods. Patients with clinical T1 renal masses were offered RPN with the XI. We used laser targeting, autopositioning, and a novel “in-line” port placement to perform RPN. Results. 15 patients underwent RPN with the XI. There were no intraoperative complications and no operative conversions. Mean console time was 101.3 minutes (range 44–176 minutes). Mean ischemia time was 17.5 minutes and estimated blood loss was 120 mLs. 12 of 15 patients had renal cell carcinoma. Two patients had oncocytoma and one had benign cystic disease. All patients had negative surgical margins and pathologic T1 disease. Two postoperative complications were encountered, including one patient who developed a pseudoaneurysm and one readmitted for presumed urinary tract infection. Conclusions. RPN with the XI system can be safely performed. Combining our surgical technique with the technological advancements on the XI offers patients acceptable pathologic and perioperative outcomes. PMID:26977144

  7. Audio annotation watermarking with robustness against DA/AD conversion

    NASA Astrophysics Data System (ADS)

    Qian, Kun; Kraetzer, Christian; Biermann, Michael; Dittmann, Jana

    2010-01-01

    In the paper we present a watermarking scheme developed to meet the specific requirements of audio annotation watermarking robust against DA/AD conversion (watermark detection after playback by loudspeaker and recording with a microphone). Additionally the described approach tries to achieve a comparably low detection complexity, so it could be embedded in the near future in low-end devices (e.g. mobile phones or other portable devices). We assume in the field of annotation watermarking that there is no specific motivation for attackers to the developed scheme. The basic idea for the watermark generation and embedding scheme is to combine traditional frequency domain spread spectrum watermarking with psychoacoustic modeling to guarantee transparency and alphabet substitution to improve the robustness. The synchronization and extraction scheme is designed to be much less computational complex than the embedder. The performance of the scheme is evaluated in the aspects of transparency, robustness, complexity and capacity. The tests reveals that 44% out of 375 tested audio files pass the simulation test for robustness, while the most appropriate category shows even 100% robustness. Additionally the introduced prototype shows an averge transparency of -1.69 in SDG, while at the same time having a capacity satisfactory to the chosen application scenario.

  8. Near-UV absorption in very cool DA white dwarfs

    SciTech Connect

    Saumon, D.; Holberg, J. B.; Kowalski, P. M. E-mail: holberg@argus.lpl.arizona.edu

    2014-07-20

    The atmospheres of very cool, hydrogen-rich white dwarfs (WDs) (T{sub eff} < 6000 K) are challenging to model because of the increased complexity of the equation of state, chemical equilibrium, and opacity sources in a low-temperature, weakly ionized dense gas. In particular, many models that assume relatively simple models for the broadening of atomic levels and mostly ideal gas physics overestimate the flux in the blue part of their spectra. A solution to this problem that has met with some success is that additional opacity at short wavelengths comes for the extreme broadening of the Lyman α line of atomic H by collisions primarily with H{sub 2}. For the purpose of validating this model more rigorously, we acquired Hubble Space Telescope STIS spectra of eight very cool WDs (five DA and three DC stars). Combined with their known parallaxes, BVRIJHK, and Spitzer IRAC photometry, we analyze their entire spectral energy distribution (from 0.24 to 9.3 μm) with a large grid of model atmospheres and synthetic spectra. We find that the red wing of the Lyman α line reproduces the rapidly decreasing near-UV flux of these very cool stars very well. We determine better constrained values of T{sub eff} and gravity as well as upper limits to the helium abundance in their atmospheres.

  9. Granite geomorphology and its geological controls, Serra da Estrela, Portugal

    NASA Astrophysics Data System (ADS)

    Migoń, Piotr; Vieira, Gonçalo

    2014-12-01

    Serra da Estrela is an elevated granite massif in central Portugal, characterized by extensive plateau surfaces incised by deep valleys affected by Quaternary glaciation, bounded by steep fault-generated escarpments. The presence of seven major textural variants of granite provides an opportunity to study the relationships between lithology and relief, whereas DEM analysis helped to show the relationships between lithology and topography objectively. The higher ground is associated with fine- to medium-grained granites and is typified by planar surfaces of low gradient, with occasional angular tors and rock pedestals. Block fields built by angular material are common in the parts that were not previously glaciated. Less elevated parts of the plateau are supported by medium- to coarse-grained granites and show more varied topography, with an abundance of tors, boulder piles, and depressions. Lithological boundaries locally coincide with slope breaks but this is not the rule. In the northern part of the massif a deep topographic basin has evolved in biotite granite, whereas deeply incised valleys follow major fault lines. Geological controls show a hierarchy, in that gross relief reflects the pattern of tectonic uplift and subsidence, whereas lithology and then fracture patterns become more and more important if one focuses on smaller and smaller landforms.

  10. DA WHITE DWARFS OBSERVED IN THE LAMOST PILOT SURVEY

    SciTech Connect

    Zhang Yueyang; Deng Licai; Liu Chao; Carrell, Kenneth; Yang Fan; Gao Shuang; Xu Yan; Li Jing; Zhang Haotong; Zhao Yongheng; Luo Ali; Bai Zhongrui; Yuan Hailong; Lepine, Sebastien; Newberg, Heidi Jo; Carlin, Jeffrey L.; Jin Ge

    2013-08-01

    A total of {approx}640, 000 objects from the LAMOST pilot survey have been publicly released. In this work, we present a catalog of DA white dwarfs (DAWDs) from the entire pilot survey. We outline a new algorithm for the selection of white dwarfs (WDs) by fitting Sersic profiles to the Balmer H{beta}, H{gamma}, and H{delta} lines of the spectra, and calculating the equivalent width of the Ca II K line. Two thousand nine hundred sixty-four candidates are selected by constraining the fitting parameters and the equivalent width of the Ca II K line. All the spectra of candidates are visually inspected. We identify 230 DAWDs (59 of which are already included in the Villanova and SDSS WD catalogs), 20 of which are DAWDs with non-degenerate companions. In addition, 128 candidates are classified as DAWDs/subdwarfs, which means the classifications are ambiguous. The result is consistent with the expected DAWD number estimated based on the LEGUE target selection algorithm.

  11. Adenovirus type 2 encoded early 11 kDa protein

    SciTech Connect

    Murthy, S.V.K.N.; Kapoor, Q.S.

    1986-05-01

    Several adenovirus type 2 (Ad2) encoded early proteins have been identified in viral infected human KB cells. These proteins are of great interest as they play key roles in cell transformation, viral DNA synthesis and gene expression. They have partially purified an AD2 encoded early polypeptide of an apparent molecular weight of 11 kilodaltons from the nuclei of viral infected cells labelled with /sup 35/S-methionine. After DNA removal from the nuclear extracts, the polypeptide was isolated using DEAE-Sephacel anion exchange and Biogel P-10 gel filtration columns. This simple two step procedure yielded several fold purification of the polypeptide. Antisera raised in mice against an Ad2 transformed rat cell line 8617 was found to immunoprecipitate the 11 kDa polypeptide from the nuclear extract of Ad2 infected KB cells. After relating this protein to an open reading frame of an Ad2 early gene block by matching the amino acid sequences to the nucleotide sequences of early genes, they plan to functionally characterize this protein by using monoclonal antibodies in in vivo and in vitro experiments.

  12. Biogeochemical Indicators of Paleoenvironmental Changes in a Humid Region of Amazonia (Lagoa da Pata, Sao Gabriel da Cachoeira)

    NASA Astrophysics Data System (ADS)

    Cordeiro, R. C.; Turcq, B.; Mendes, L. G.; Azeredo, J. B.; Moura, M. S.; de Oliveira, A. R.; Monteiro, F.; Santelli, R. E.

    2004-12-01

    Analysis of four sediment core collected in Lagoa da Pata lake show significant changes in the environmental history of Amazonia during the last 40,000 years. The Lagoa da Pata Lake (0°17'S, 66°40'W) is located on the top of the Morro dos Seis Lagos, an isolated hill in the extensive forested plane surface of high Rio Negro basin. The climate in the region presents mean annual precipitation around 3000 mm without dry season. Three distinct sections are clearly identified in the LPT III, LPT IV and LPT V cores. They consist of upper and lower organic-rich layers, separated by a clastic layer which represents a short period of rapid accumulation around 18 ka BP. Analysis of the organic matter composition by d13C and d15N, total Carbon and Nitrogen, sedimentary chlorophyll concentration and organic matter petrography show three different phases related to organic matter deposition. Between, at least 46,000 and 31,000 14C yr B.P., the lake presented a high water level attested by high accumulation rates of organic carbon. Values of d13C (around -32%) associated to high C/N ratio demonstrate a lignocellulosic material contribution in this phase; the second phase between the 31000 yr 14C B.P. and 18000 14C B.P. is characterised by a lower productivity attested by low sedimentary chlorophyll and total organic carbon values. The d13C values increase with medium values around -28% that may correspond to an input of organic matter rich in C4 plants (grasses). The sedimentation rate decreases significantly. High charcoal fluxes were observed in this period. At around 18000 14C yr BP, there appears to have been a sudden input of clastic material. This is represented by a sandy facies, which exhibits lower carbon contents. From 18000 14C yr B.P. to the present time occurred an increase in lacustrine productivity marked by an increase in carbon and chlorophyll derivate accumulation rates. The C/N values dropped indicting an algae organic matter contribution. It probably

  13. Temperature and Gravity Dependence of Trace Element Abundances in Hot DA White Dwarfs (94-EUVE-094)

    NASA Technical Reports Server (NTRS)

    Finley, David S.

    1998-01-01

    EUV spectroscopy has shown that DA white dwarfs hotter than about 45,000 K may contain trace heavy elements, while those hotter than about 50,000 K almost always have significant abundances of trace heavy elements. One of our continuing challenges is to identify and determine the abundances of these trace constituents, and then to relate the observed abundance patterns to the present conditions and previous evolutionary histories of the hot DA white dwarfs.

  14. In vitro degradation of the 32kDa PS II reaction centre protein

    SciTech Connect

    Eckenswiller, L.C.; Greenberg, B.M. )

    1989-04-01

    The 32kDa thylakoid membrane protein is an integral component of the PS II reaction centre. The protein, although stable in the dark, undergoes light dependent turnover. Light from the UV, visible and far-red spectral regions induce 32kDa protein degradation. To better understand 32kDa protein metabolism, an in vitro degradation system is being developed. It consists of isolated thylakoid membranes than contain radiolabelled protein. The 32kDa protein is actively and specifically degraded when the thylakoid preparation is exposed to UV or visible radiation. The protein is stable in the dark. The herbicides (atrazine and DCMU) inhibit degradation in the in vitro system as they do in vivo. Additionally, several methods of isolating thylakoids are being compared to optimize the 32kDa protein degradation reaction. The preparations will be evaluated based on their ability to permit light dependent degradation of the 32kDa protein without affecting the other membrane components.

  15. The 32 kDa Enamelin Undergoes Conformational Transitions upon Calcium Binding

    PubMed Central

    Fan, Daming; Lakshminarayanan, Rajamani; Moradian-Oldak, Janet

    2008-01-01

    The 32 kDa hydrophilic and acidic enamelin, the most stable cleavage fragment of the enamel specific glycoprotein, is believed to play vital roles in controlling crystal nucleation or growth during enamel biomineralization. Circular dichroism and Fourier transform infrared spectra demonstrate that the secondary structure of the 32 kDa enamelin has a high content of α-helix (81.5%). Quantitative analysis on the circular dichroism data revealed that the 32 kDa enamelin undergoes conformational changes with a structural preference to β-sheet as a function of calcium ions. We suggest that the increase of β-sheet conformation upon presence of Ca2+ may allow preferable interaction of the 32 kDa enamelin with apatite crystal surfaces during enamel biomineralization. The calcium association constant of the 32 kDa enamelin calculated from the fitting curve of ellipticity at 222 nm is Ka = 1.55 (±0.13) × 103 M−1, indicating a relatively low affinity. Our current biophysical studies on the 32 kDa enamelin structure provide novel insights towards understanding the enamelin-mineral interaction and subsequently the functions of enamelin during enamel formation. PMID:18508280

  16. OpenDA: Open Source Generic Data Assimilation Environment and its Application in Geophysical Process Models

    NASA Astrophysics Data System (ADS)

    Weerts, A.; van Velzen, N.; Verlaan, M.; Sumihar, J.; Hummel, S.; El Serafy, G.; Dhondia, J.; Gerritsen, H.; Vermeer-Ooms, S.; Loots, E.; Markus, A.; Kockx, A.

    2011-12-01

    Data Assimilation techniques are essential elements in state-of-the-art development of models and their use in operational forecasting and real-time control in the fields of groundwater, surface water and soil systems. In meteorological and atmospheric sciences, steady improvements in numerical weather forecasting and climate prediction over the last couple of decades have been enabled to a large degree by the development of community-based models and data assimilation systems. The hydrologic community should learn from the experiences of the meteorological and atmospheric communities by accelerating the transition of hydrologic DA research into operations and developing community-supported, open-source modeling and forecasting systems and data assimilation tools. In 2010, a community based open source initiative named OpenDA was started. The openDA initiative bears similarities with the well-known openMI initiative. OpenDA makes use of a set of interfaces that describe the interaction between models, observations and data assimilation algorithms. It focuses on flexible applications in portable systems for modeling geophysical processes. It provides a generic interfacing protocol that allows combination of the implemented data assimilation techniques with, in principle, any time-stepping model describing a process (atmospheric processes, 3D circulation, 2D water flow, rainfall-runoff, unsaturated flow, groundwater flow, etc.). Presently, openDA features filtering techniques and calibration techniques. The presentation will give an overview of openDA and the results of some of its practical applications.

  17. [The behavior of superoxide dismutase (SOD) in serum of cows with abomasal displacement (DA)].

    PubMed

    Fürll, M; Dabbagh, M N; Fürll, B; Sattler, T

    2004-01-01

    After surgical reposition of displaced organs (abomasum, uterus, intestines) restoration of blood flow and oxygen supply generates oxygen radicals and other reactive oxygen species. SOD indicates radical stress of the organism. Subject of the study was the question if SOD can be detected in blood serum samples of cows and if there are differences in SOD activity between healthy cows and cows with Dislocatio abomasi (DA). We also wanted to investigate the influence of breed "Schwarzbunte" with DA (16 left/5 rights). The samples were drawn before and 1, 3 and 24 post op. Ten healthy cows of the same breed were also examined (2 weeks and 4-6 weeks after calving). There are no significant differences between the SOD activity of healthy cows and cows with DA, but the SOD activity of cows with left DA is significant lower than the activity of cows with right DA. Post op. SOD activity decreases; 24 h after surgery cows with left but not with right DA show an increase of SOD activity similar to values before surgery. There is a close positive correlation between SOD activity and protein concentration as well as negative correlation to concentration of free fatty acids after surgery. The behaviour of SOD activity shows that the surgical replacement of the displaced abomasum can generate a depression of the antioxidative capacity of the organism. PMID:14983749

  18. The sandfly fauna (Diptera: Psychodidae: Phlebotominae) of the Parque Estadual da Serra da Tiririca, Rio de Janeiro, Brazil

    PubMed Central

    Rodrigues, Andressa Alencastre Fuzari; Barbosa, Vanessa de Araújo; Andrade, José Dilermando; Brazil, Reginaldo Peçanha

    2013-01-01

    Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can be characterised as a peridomestic transmission that occurs in modified natural environments. The aim of this work was to study the fauna and ecological characteristics of sandflies in an environmentally protected area (the State Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the municipalities of Niterói and Maricá and their possible relationship with leishmaniasis. Captures were performed using light traps during the night once a month for one year in both sylvatic environments and areas surrounding homes near the park. A total of 1,037 sandflies were captured, belonging to nine genera and 12 species: Evandromyia tupynambai (34.1%), Migonemyia migonei (20.6%), Brumptomyia cunhai (13.8%), Micropygomyia schreiberi (9.7%), Psathyromyia lanei (6.5%), Brumptomyia nitzulescui (5.7%), Evandromyia edwardsi (5.4%), Nyssomyia intermedia (2.8%), Evandromyia cortelezzii (0.6%), Pintomyia bianchigalatiae (0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia microps (0.1%). Both Mg. migonei and Ny. intermedia may be acting as vectors of CL in this area. PMID:24141956

  19. Negative regulation of human immunodeficiency virus type 1 expression in monocytes: role of the 65-kDa plus 50-kDa NF-kappa B dimer.

    PubMed

    Raziuddin; Mikovits, J A; Calvert, I; Ghosh, S; Kung, H F; Ruscetti, F W

    1991-11-01

    Although monocytic cells can provide a reservoir for viral production in vivo, their regulation of human immunodeficiency virus type 1 (HIV-1) transcription can be either latent, restricted, or productive. These differences in gene expression have not been molecularly defined. In THP-1 cells with restricted HIV expression, there is an absence of DNA-protein binding complex formation with the HIV-1 promoter-enhancer associated with markedly less viral RNA production. This absence of binding was localized to the NF-kappa B region of the HIV-1 enhancer; the 65-kDa plus 50-kDa NF-kappa B heterodimer was preferentially lost. Adding purified NF-kappa B protein to nuclear extracts from cells with restricted expression overcomes this lack of binding. In addition, treatment of these nuclear extracts with sodium deoxycholate restored their ability to form the heterodimer, suggesting the presence of an inhibitor of NF-kappa B activity. Furthermore, treatment of nuclear extracts from these cells that had restricted expression with lipopolysaccharide increased viral production and NF-kappa B activity. Antiserum specific for NF-kappa B binding proteins, but not c-rel-specific antiserum, disrupted heterodimer complex formation. Thus, both NF-kappa B-binding complexes are needed for optimal viral transcription. Binding of the 65-kDa plus 50-kDa heterodimer to the HIV-1 enhancer can be negatively regulated in monocytes, providing one mechanism restricting HIV-1 gene expression. PMID:1946356

  20. Myelin management by the 18.5–kDa and 21.5–kDa classic myelin basic protein isoforms

    PubMed Central

    Harauz, George; Boggs, Joan M.

    2013-01-01

    The classic myelin basic protein (MBP) splice isoforms range in nominal molecular mass from 14 to 21.5 kDa, and arise from the gene in the oligodendrocyte lineage (Golli) in maturing oligodendrocytes. The 18.5-kDa isoform that predominates in adult myelin adheres the cytosolic surfaces of oligodendrocyte membranes together, and forms a two-dimensional molecular sieve restricting protein diffusion into compact myelin. However, this protein has additional roles including cytoskeletal assembly and membrane extension, binding to SH3-domains, participation in Fyn-mediated signaling pathways, sequestration of phosphoinositides, and maintenance of calcium homeostasis. Of the diverse post-translational modifications of this isoform, phosphorylation is the most dynamic, and modulates 18.5-kDa MBP’s protein-membrane and protein-protein interactions, indicative of a rich repertoire of functions. In developing and mature myelin, phosphorylation can result in microdomain or even nuclear targeting of the protein, supporting the conclusion that 18.5-kDa MBP has significant roles beyond membrane adhesion. The full-length, early-developmental 21.5-kDa splice isoform is predominantly karyophilic due to a non-traditional P-Y nuclear localization signal, with effects such as promotion of oligodendrocyte proliferation. We discuss in vitro and recent in vivo evidence for multifunctionality of these classic basic proteins of myelin, and argue for a systematic evaluation of the temporal and spatial distributions of these protein isoforms, and their modified variants, during oligodendrocyte differentiation. PMID:23398367

  1. Characteristics of 26 kDa antigen of H. Pylori by Monoclonal Antibody.

    PubMed

    Ghahremani, Hossein; Farshad, Shohreh; Amini Najafabadi, Hossein; Kashanian, Susan; Momeni Moghaddam, Mohammad Amin; Moradi, Nariman; Paknejad, Maliheh

    2015-02-01

    Alkylhydroperoxide reductase (AhpC, the 26 kDa antigen) is one of the abundant antioxidant enzymes in Helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect H. pylori infection in clinical specimens. This study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. Five established hybridoma cell lines secreting monoclonal antibodies (MAbs) against 26 kDa antigen of H. pylori were cultivated and MAbs were purified by affinity chromatography. Subsequently, MAbs were conjugated with biotin, and different combinations of capture and tracer antibodies used in sandwich ELISA. Immunoblotting of bacterial extracts were performed to estimate aggregation status of the antigen. Release of antigen from the cultivated bacteria on solid media was examined by sandwich ELISA, and also, existence of interference in fecal extract was investigated by immunoblotting and sandwich ELISA. Our findings showed that the MAbs against 26 kDa antigen of H. pylori could recognize three bands of nearly 25 kDa, 50 kDa, and 75 kDa in immunoblotting. This study also indicated presence of more antigens in the culture medium around the bacteria than the bacterial extract itself. The results of sandwich ELISA and immunoblotting on fecal extracts suggest the presence of interfering agents that prevent detection of antigen by antibody in ELISA but not in immunoblotting. In this study the oligomerization of the 26 kDa antigen, presence of interfering agents in stool matrix, and release of antigen to outside of bacteria, were demonstrated. PMID:25530147

  2. Naloxone-induced jumping activity in morphine-dependent mice: a pharmacological study on the role of DA2 receptors.

    PubMed

    Cerbo, R; Boni, B; De Lena, C; Cesarino, F; Meco, G; Feliciani, M; Formisano, R; Casacchia, M

    1984-03-30

    The morphine withdrawal syndrome is strictly related to modifications on neurotransmittorial systems at central and peripheral levels. Dopamine seems to play an important role. Particularly in the experimental withdrawal syndrome the jumping activity seems to be related to a hypersensitivity of DA receptors. While the role of DA1 receptors has been extensively studied, using agonist and antagonist drugs, the role of DA2 receptors is still little known. In our study we evaluated the changes of the jumping response that was induced by substances active on DA2 receptors (lisuride, tiapride) in mice made acutely dependent on morphine, in which we induced a withdrawal syndrome with naloxone. Haloperidol (DA1-DA2 blocker) did not provoke significant changes. Either tiapride or lisuride determined a sharp increase of jumping. PMID:6712813

  3. 82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.

    PubMed

    Winick-Ng, Warren; Caetano, Fabiana A; Winick-Ng, Jennifer; Morey, Trevor M; Heit, Bryan; Rylett, R Jane

    2016-01-01

    The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1-42 (Aβ1-42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1-42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generation sequencing (ChIP-seq), we identified that Aβ1-42-exposure increases 82-kDa ChAT association with gene promoters and introns. The Aβ1-42-induced 82-kDa ChAT aggregates co-localize with special AT-rich binding protein 1 (SATB1), which anchors DNA to scaffolding/matrix attachment regions (S/MARs). SATB1 had a similar genomic association as 82-kDa ChAT, with both proteins associating with synapse and cell stress genes. After Aβ1-42 -exposure, both SATB1 and 82-kDa ChAT are enriched at the same S/MAR on the APP gene, with 82-kDa ChAT expression attenuating an increase in an isoform-specific APP mRNA transcript. Finally, 82-kDa ChAT and SATB1 have patterned genomic association at regions enriched with S/MAR binding motifs. These results demonstrate that 82-kDa ChAT and SATB1 play critical roles in the response of neural cells to acute Aβ-exposure. PMID:27052102

  4. An Introduction to the DA-T Gibbs Sampler for the Two-Parameter Logistic (2PL) Model and beyond

    ERIC Educational Resources Information Center

    Maris, Gunter; Bechger, Timo M.

    2005-01-01

    The DA-T Gibbs sampler is proposed by Maris and Maris (2002) as a Bayesian estimation method for a wide variety of "Item Response Theory (IRT) models". The present paper provides an expository account of the DA-T Gibbs sampler for the 2PL model. However, the scope is not limited to the 2PL model. It is demonstrated how the DA-T Gibbs sampler for…

  5. 11 CFR 111.11 - Written questions under order (2 U.S.C. 437d(a)(1)).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 11 Federal Elections 1 2011-01-01 2011-01-01 false Written questions under order (2 U.S.C. 437d(a... PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.11 Written questions under order (2 U.S.C. 437d(a)(1... submit sworn written answers to written questions and may specify a date by which such answers must...

  6. 11 CFR 111.11 - Written questions under order (2 U.S.C. 437d(a)(1)).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Written questions under order (2 U.S.C. 437d(a... PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.11 Written questions under order (2 U.S.C. 437d(a)(1... submit sworn written answers to written questions and may specify a date by which such answers must...

  7. 82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions

    PubMed Central

    Winick-Ng, Warren; Caetano, Fabiana A.; Winick-Ng, Jennifer; Morey, Trevor M.; Heit, Bryan; Rylett, R. Jane

    2016-01-01

    The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1–42 (Aβ1–42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1–42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generation sequencing (ChIP-seq), we identified that Aβ1–42 -exposure increases 82-kDa ChAT association with gene promoters and introns. The Aβ1–42 -induced 82-kDa ChAT aggregates co-localize with special AT-rich binding protein 1 (SATB1), which anchors DNA to scaffolding/matrix attachment regions (S/MARs). SATB1 had a similar genomic association as 82-kDa ChAT, with both proteins associating with synapse and cell stress genes. After Aβ1–42 -exposure, both SATB1 and 82-kDa ChAT are enriched at the same S/MAR on the APP gene, with 82-kDa ChAT expression attenuating an increase in an isoform-specific APP mRNA transcript. Finally, 82-kDa ChAT and SATB1 have patterned genomic association at regions enriched with S/MAR binding motifs. These results demonstrate that 82-kDa ChAT and SATB1 play critical roles in the response of neural cells to acute Aβ -exposure. PMID:27052102

  8. Characterization of the 105-kDa molecular chaperone. Identification, biochemical properties, and localization.

    PubMed

    Matsumori, Mika; Itoh, Hideaki; Toyoshima, Itaru; Komatsuda, Atsushi; Sawada, Ken-Ichi; Fukuda, Jun; Tanaka, Toshinobu; Okubo, Atsuya; Kinouchi, Hiroyuki; Mizoi, Kazuo; Hama, Tokiko; Suzuki, Akira; Hamada, Fumio; Otaka, Michiro; Shoji, Yutaka; Takada, Goro

    2002-11-01

    We have characterized the biochemical properties of the testis and brain-specific 105-kDa protein which is cross-reacted with an anti-bovine HSP90 antibody. The protein was induced in germ cells by heat stress, resulting in a protein which is one of the heat shock proteins [Kumagai, J., Fukuda, J., Kodama, H., Murata, M., Kawamura, K., Itoh, H. & Tanaka, T. (2000) Eur. J. Biochem.267, 3073-3078]. In the present study, we characterized the biochemical properties of the protein. The 105-kDa protein inhibited the aggregation of citrate synthase as a molecular chaperone in vitro. ATP/MgCl2 has a slight influence of the suppression of the citrate synthase aggregation by the 105-kDa protein. The protein possessed chaperone activity. The protein was able to bind to ATP-Sepharose like the other molecular chaperone HSP70. A partial amino-acid sequence (24 amino-acid residues) of the protein was determined and coincided with those of the mouse testis- and brain-specific APG-1 and osmotic stress protein 94 (OSP94). The 105-kDa protein was detected only in the medulla of the rat kidney sections similar to OSP94 upon immunoblotting. The purified 105-kDa protein was cross-reacted with an antibody against APG-1. These results suggested that APG-1 and OSP94 are both identical to the 105-kDa protein. There were highly homologous regions between the 105-kDa protein/APG-1/OSP94 and HSP90. The region of HSP90 was also an immunoreactive site. An anti-bovine HSP90 antibody may cross-react with the 105-kDa protein similar to HSP90 in the rat testis and brain. We have investigated the localization and developmental induction of the protein in the rat brain. In the immunohistochemical analysis, the protein was mainly detected in the cytoplasm of the nerve and glial cells of the rat brain. Although the 105-kDa protein was localized in all rat brain segments, the expression pattern was fast in the cerebral cortex and hippocampus and slow in the cerebellum. PMID:12423363

  9. High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster

    PubMed Central

    2014-01-01

    Background The Drosophila melanogaster Serpin 42 Da gene (previously Serpin 4) encodes a serine protease inhibitor that is capable of remarkable functional diversity through the alternative splicing of four different reactive centre loop exons. Eight protein isoforms of Serpin 42 Da have been identified to date, targeting the protease inhibitor to both different proteases and cellular locations. Biochemical and genetic studies suggest that Serpin 42 Da inhibits target proteases through the classical serpin ‘suicide’ inhibition mechanism, however the crystal structure of a representative Serpin 42 Da isoform remains to be determined. Results We report two high-resolution crystal structures of Serpin 42 Da representing the A/B isoforms in the cleaved conformation, belonging to two different space-groups and diffracting to 1.7 Å and 1.8 Å. Structural analysis reveals the archetypal serpin fold, with the major elements of secondary structure displaying significant homology to the vertebrate serpin, neuroserpin. Key residues known to have central roles in the serpin inhibitory mechanism are conserved in both the hinge and shutter regions of Serpin 42 Da. Furthermore, these structures identify important conserved interactions that appear to be of crucial importance in allowing the Serpin 42 Da fold to act as a versatile template for multiple reactive centre loops that have different sequences and protease specificities. Conclusions In combination with previous biochemical and genetic studies, these structures confirm for the first time that the Serpin 42 Da isoforms are typical inhibitory serpin family members with the conserved serpin fold and inhibitory mechanism. Additionally, these data reveal the remarkable structural plasticity of serpins, whereby the basic fold is harnessed as a template for inhibition of a large spectrum of proteases by reactive centre loop exon ‘switching’. This is the first structure of a Drosophila serpin reported to date

  10. The rare DAT coding variant Val559 perturbs DA neuron function, changes behavior, and alters in vivo responses to psychostimulants

    PubMed Central

    Mergy, Marc A.; Gowrishankar, Raajaram; Gresch, Paul J.; Gantz, Stephanie C.; Williams, John; Davis, Gwynne L.; Wheeler, C. Austin; Stanwood, Gregg D.; Hahn, Maureen K.; Blakely, Randy D.

    2014-01-01

    Despite the critical role of the presynaptic dopamine (DA) transporter (DAT, SLC6A3) in DA clearance and psychostimulant responses, evidence that DAT dysfunction supports risk for mental illness is indirect. Recently, we identified a rare, nonsynonymous Slc6a3 variant that produces the DAT substitution Ala559Val in two male siblings who share a diagnosis of attention-deficit hyperactivity disorder (ADHD), with other studies identifying the variant in subjects with bipolar disorder (BPD) and autism spectrum disorder (ASD). Previously, using transfected cell studies, we observed that although DAT Val559 displays normal total and surface DAT protein levels, and normal DA recognition and uptake, the variant transporter exhibits anomalous DA efflux (ADE) and lacks capacity for amphetamine (AMPH)-stimulated DA release. To pursue the significance of these findings in vivo, we engineered DAT Val559 knock-in mice, and here we demonstrate in this model the presence of elevated extracellular DA levels, altered somatodendritic and presynaptic D2 DA receptor (D2R) function, a blunted ability of DA terminals to support depolarization and AMPH-evoked DA release, and disruptions in basal and psychostimulant-evoked locomotor behavior. Together, our studies demonstrate an in vivo functional impact of the DAT Val559 variant, providing support for the ability of DAT dysfunction to impact risk for mental illness. PMID:25331903

  11. The rare DAT coding variant Val559 perturbs DA neuron function, changes behavior, and alters in vivo responses to psychostimulants.

    PubMed

    Mergy, Marc A; Gowrishankar, Raajaram; Gresch, Paul J; Gantz, Stephanie C; Williams, John; Davis, Gwynne L; Wheeler, C Austin; Stanwood, Gregg D; Hahn, Maureen K; Blakely, Randy D

    2014-11-01

    Despite the critical role of the presynaptic dopamine (DA) transporter (DAT, SLC6A3) in DA clearance and psychostimulant responses, evidence that DAT dysfunction supports risk for mental illness is indirect. Recently, we identified a rare, nonsynonymous Slc6a3 variant that produces the DAT substitution Ala559Val in two male siblings who share a diagnosis of attention-deficit hyperactivity disorder (ADHD), with other studies identifying the variant in subjects with bipolar disorder (BPD) and autism spectrum disorder (ASD). Previously, using transfected cell studies, we observed that although DAT Val559 displays normal total and surface DAT protein levels, and normal DA recognition and uptake, the variant transporter exhibits anomalous DA efflux (ADE) and lacks capacity for amphetamine (AMPH)-stimulated DA release. To pursue the significance of these findings in vivo, we engineered DAT Val559 knock-in mice, and here we demonstrate in this model the presence of elevated extracellular DA levels, altered somatodendritic and presynaptic D2 DA receptor (D2R) function, a blunted ability of DA terminals to support depolarization and AMPH-evoked DA release, and disruptions in basal and psychostimulant-evoked locomotor behavior. Together, our studies demonstrate an in vivo functional impact of the DAT Val559 variant, providing support for the ability of DAT dysfunction to impact risk for mental illness. PMID:25331903

  12. A Influência da Escola e da Mídia na Obtenção dos Conhecimentos Astronômicos

    NASA Astrophysics Data System (ADS)

    da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.

    2006-08-01

    O universo em que vivemos possui segredos jamais imaginados pelo homem, fruto da pequena proporção que ocupamos diante de um cosmos infinito. A astronomia funciona como um instrumento que sacia a sede humana em desvendar esses segredos, estudando as galáxias, quasares, pulsares além da existência de todos os elementos que preenchem o espaço cósmico, o vácuo, não mais vazio como se pensou em outros tempos. A mídia explora esse campo de forma mística e também científica. A difusão adequada e dentro dos padrões educacionais vigentes, de conceitos astronômicos, se faz necessária, devido sua presença nos conteúdos escolares, centros de ciência e na mídia, de modo geral. A reforma do Currículo Básico da Escola Pública da maioria dos Estados brasileiros introduziu conceitos astronômicos desde a pré-escola até o Ensino Médio. Num estudo realizado com 1.032 alunos do Ensino Médio de seis escolas estaduais, revelou-se através da aplicação de um questionário que abordava conheciment os básicos em astronomia, como estes foram adquiridos e qual era a infra-estrutura oferecida pela escola: para 66,2% dos entrevistados o professor já fez alguma apresentação a respeito de astronomia. O presente estudo revelou também que 44,5% dos alunos entrevistados adquiriram seus conhecimentos astronômicos na escola, 28,1% através da televisão e 27,4% afirmaram que seus conhecimentos foram obtidos através de outros tipos de mídia (internet, filmes e revistas). Embora o estudo revele claramente que há certo equilíbrio na forma em que os alunos obtiveram seus conhecimentos astronômicos, a mídia ainda explora e muitas vezes desperta o interesse pela astronomia com assuntos que não fazem parte de nosso cotidiano como, por exemplo, o homem na Lua, os projetos espaciais e a primeira viagem de um astronauta brasileiro no espaço. Porém, em certos casos, a mídia pode promover conhecimentos incompletos ou mesmo equivocados sobre os conceitos que envolvam

  13. Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase

    SciTech Connect

    Shoseyov, O.; Doi, R.H. )

    1990-03-01

    The cellulase complex from Clostridium cellulovorans has been purified and its subunit composition determined. The complex exhibits cellulase activity against crystalline cellulose as well as carboxymethylcellulase (CMCase) and cellobiohydrolase activities. Three major subunits are present with molecular masses of 170, 100, and 70 kDa. The 100-kDa subunit is the major CMCase, although at least four other, minor subunits show CMCase activity. The 170-kDa subunit has the highest affinity for cellulose, does not have detectable enzymatic activity, but is necessary for cellulase activity. Immunological studies indicate that the 170-kDa subunit is not required for binding of the catalytic subunits to cellulose and therefore does not function solely as an anchor protein. Thus this core subunit must have multiple functions. The authors propose a working hypothesis that the binding of the 170-kDa subunit converts the crystalline cellulose to a form that is capable of being hydrolyzed in a cooperative fashion by the associated catalytic subunits.

  14. Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis

    PubMed Central

    Song, Hyun-Ouk

    2016-01-01

    Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis. PMID:26951982

  15. Experience with ATLAS MySQL PanDA database service

    NASA Astrophysics Data System (ADS)

    Smirnov, Y.; Wlodek, T.; De, K.; Hover, J.; Ozturk, N.; Smith, J.; Wenaus, T.; Yu, D.

    2010-04-01

    The PanDA distributed production and analysis system has been in production use for ATLAS data processing and analysis since late 2005 in the US, and globally throughout ATLAS since early 2008. Its core architecture is based on a set of stateless web services served by Apache and backed by a suite of MySQL databases that are the repository for all PanDA information: active and archival job queues, dataset and file catalogs, site configuration information, monitoring information, system control parameters, and so on. This database system is one of the most critical components of PanDA, and has successfully delivered the functional and scaling performance required by PanDA, currently operating at a scale of half a million jobs per week, with much growth still to come. In this paper we describe the design and implementation of the PanDA database system, its architecture of MySQL servers deployed at BNL and CERN, backup strategy and monitoring tools. The system has been developed, thoroughly tested, and brought to production to provide highly reliable, scalable, flexible and available database services for ATLAS Monte Carlo production, reconstruction and physics analysis.

  16. Synergistic effects of melatonin and deprenyl against MPTP-induced mitochondrial damage and DA depletion.

    PubMed

    Khaldy, Hoda; Escames, Germaine; León, Josefa; Bikjdaouene, Leila; Acuña-Castroviejo, Darío

    2003-01-01

    Previous studies showed a synergistic effect of melatonin and deprenyl against dopamine (DA) autoxidation in vitro. Since oxidative stress is implicated in Parkinson's disease (PD), we explored the effects of melatonin plus deprenyl administration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD in C57/Bl6 mice. Melatonin, but not deprenyl prevents the inhibition of mitochondrial complex I and the oxidative damage in nigrostriatal neurons induced by MPTP. With the dose used deprenyl recovers 50% DA levels and tyrosine hydroxylase activity depressed by the neurotoxin, normalizing locomotor activity of mice. Melatonin, which was unable to counteract MPTP-induced DA depletion and inhibition of tyrosine hydroxylase activity, potentiates the effect of deprenyl on catecholamine turnover and mice ambulatory activity. These results suggest a dissociation of complex I inhibition from DA depletion in this model of Parkinson's disease. The data also support that a combination of melatonin, which improves mitochondrial electron transport chain and reduces oxidative damage, and deprenyl, which promotes the specific function of the rescued neurons, i.e. DA turnover, may be a promising strategy for the treatment of PD. PMID:12600724

  17. In vitro study on the interaction between the 32 kDa enamelin and amelogenin

    PubMed Central

    Fan, Daming; Du, Chang; Sun, Zhi; Lakshminarayanan, Rajamani; Moradian-Oldak, Janet

    2015-01-01

    Enamel extracelluar matrix components play vital roles in controlling crystal nucleation and growth during enamel formation. We investigated the interaction between the 32 kDa enamelin fragment and amelogenin using immunochemical and biophysical methods. Immunoprecipitation studies revealed that the 32 kDa enamelin and amelogenin eluted together from a Protein A column. Dynamic light scattering results showed that the 32 kDa enamelin had a profound effect on amelogenin assembly at pH 8.0, causing partial dissociation of the nanospheres, in a dose-dependent manner. The appearance of an isodichroic point and the shifting and intensity decrease of the ellipticity minima in the circular dichroism spectra of amelogenin following the addition of the 32 kDa enamelin were indicative of conformational changes in amelogenin and of a direct interaction between the two macromolecules. Our results collectively demonstrate that the 32 kDa enamelin has a direct interaction with amelogenin in vitro. Our current studies provide novel insights into understanding possible cooperation between enamelin and amelogenin in macromolecular self-assembly and in controlling enamel mineral formation. PMID:19263522

  18. Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron.

    PubMed

    Shen, Hung-Chang; Wei, Jia-Yi; Chu, Sao-Yu; Chung, Pei-Chi; Hsu, Tsai-Chi; Yu, Hung-Hsiang

    2016-01-01

    In the Drosophila olfactory system, odorant information is sensed by olfactory sensory neurons and relayed from the primary olfactory center, the antennal lobe (AL), to higher olfactory centers via olfactory projection neurons (PNs). A major portion of the AL is constituted with dendrites of four groups of PNs, anterodorsal PNs (adPNs), lateral PNs (lPNs), lateroventral PNs (lvPNs) and ventral PNs (vPNs). Previous studies have been focused on the development and function of adPNs and lPNs, while the investigation on those of lvPNs and vPNs received less attention. Here, we study the molecular and cellular mechanisms underlying the morphogenesis of a putative male-pheromone responding vPN, the DA1 vPN. Using an intersection strategy to remove background neurons labeled within a DA1 vPN-containing GAL4 line, we depicted morphological changes of the DA1 vPN that occurs at the pupal stage. We then conducted a pilot screen using RNA interference knock-down approach to identify cell surface molecules, including Down syndrome cell adhesion molecule 1 and Semaphorin-1a, that might play essential roles for the DA1 vPN morphogenesis. Taken together, by revealing molecular and cellular basis of the DA1 vPN morphogenesis, we should provide insights into future comprehension of how vPNs are assembled into the olfactory neural circuitry. PMID:27163287

  19. Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron

    PubMed Central

    Yu, Hung-Hsiang

    2016-01-01

    In the Drosophila olfactory system, odorant information is sensed by olfactory sensory neurons and relayed from the primary olfactory center, the antennal lobe (AL), to higher olfactory centers via olfactory projection neurons (PNs). A major portion of the AL is constituted with dendrites of four groups of PNs, anterodorsal PNs (adPNs), lateral PNs (lPNs), lateroventral PNs (lvPNs) and ventral PNs (vPNs). Previous studies have been focused on the development and function of adPNs and lPNs, while the investigation on those of lvPNs and vPNs received less attention. Here, we study the molecular and cellular mechanisms underlying the morphogenesis of a putative male-pheromone responding vPN, the DA1 vPN. Using an intersection strategy to remove background neurons labeled within a DA1 vPN-containing GAL4 line, we depicted morphological changes of the DA1 vPN that occurs at the pupal stage. We then conducted a pilot screen using RNA interference knock-down approach to identify cell surface molecules, including Down syndrome cell adhesion molecule 1 and Semaphorin-1a, that might play essential roles for the DA1 vPN morphogenesis. Taken together, by revealing molecular and cellular basis of the DA1 vPN morphogenesis, we should provide insights into future comprehension of how vPNs are assembled into the olfactory neural circuitry. PMID:27163287

  20. Low strain, long life creep fatigue of AF2-1DA and INCO 718

    SciTech Connect

    Thakker, A.B.; Cowles, B.A.

    1983-04-01

    Two aircraft turbine disk alloys, GATORIZED AF2-DA and INCO 718 were evaluated for their low strain long life creep-fatigue behavior. Static (tensile and creep rupture) and cyclic properties of both alloys were characterized. The cntrolled strain LCF tests were conducted at 760 C (1400 F) and 649 C (1200 F) for AF2-1DA and INCO 718, respectively. Hold times were varied for tensile, compressive and tensile/compressive strain dwell (relaxation) tests. Stress (creep) hold behavior of AF2-1DA was also evaluated. Generally, INCO 718 exhibited more pronounced reduction in cyclic life due to hold than AF2-1DA. The percent reduction in life for both alloys for strain dwell tests was greater at low strain ranges (longer life regime). Changing hold time from 0 to 0.5, 2.0 and 15.0 min. resulted in corresponding reductions in life. The continuous cycle and cyclic/dwell initiation failure mechanism was predominantly transgranular for AF2-1DA and intergranular for INCO 718.

  1. PanDA: Exascale Federation of Resources for the ATLAS Experiment at the LHC

    NASA Astrophysics Data System (ADS)

    Barreiro Megino, Fernando; Caballero Bejar, Jose; De, Kaushik; Hover, John; Klimentov, Alexei; Maeno, Tadashi; Nilsson, Paul; Oleynik, Danila; Padolski, Siarhei; Panitkin, Sergey; Petrosyan, Artem; Wenaus, Torre

    2016-02-01

    After a scheduled maintenance and upgrade period, the world's largest and most powerful machine - the Large Hadron Collider(LHC) - is about to enter its second run at unprecedented energies. In order to exploit the scientific potential of the machine, the experiments at the LHC face computational challenges with enormous data volumes that need to be analysed by thousand of physics users and compared to simulated data. Given diverse funding constraints, the computational resources for the LHC have been deployed in a worldwide mesh of data centres, connected to each other through Grid technologies. The PanDA (Production and Distributed Analysis) system was developed in 2005 for the ATLAS experiment on top of this heterogeneous infrastructure to seamlessly integrate the computational resources and give the users the feeling of a unique system. Since its origins, PanDA has evolved together with upcoming computing paradigms in and outside HEP, such as changes in the networking model, Cloud Computing and HPC. It is currently running steadily up to 200 thousand simultaneous cores (limited by the available resources for ATLAS), up to two million aggregated jobs per day and processes over an exabyte of data per year. The success of PanDA in ATLAS is triggering the widespread adoption and testing by other experiments. In this contribution we will give an overview of the PanDA components and focus on the new features and upcoming challenges that are relevant to the next decade of distributed computing workload management using PanDA.

  2. Low strain, long life creep fatigue of AF2-1DA and INCO 718

    NASA Technical Reports Server (NTRS)

    Thakker, A. B.; Cowles, B. A.

    1983-01-01

    Two aircraft turbine disk alloys, GATORIZED AF2-DA and INCO 718 were evaluated for their low strain long life creep-fatigue behavior. Static (tensile and creep rupture) and cyclic properties of both alloys were characterized. The cntrolled strain LCF tests were conducted at 760 C (1400 F) and 649 C (1200 F) for AF2-1DA and INCO 718, respectively. Hold times were varied for tensile, compressive and tensile/compressive strain dwell (relaxation) tests. Stress (creep) hold behavior of AF2-1DA was also evaluated. Generally, INCO 718 exhibited more pronounced reduction in cyclic life due to hold than AF2-1DA. The percent reduction in life for both alloys for strain dwell tests was greater at low strain ranges (longer life regime). Changing hold time from 0 to 0.5, 2.0 and 15.0 min. resulted in corresponding reductions in life. The continuous cycle and cyclic/dwell initiation failure mechanism was predominantly transgranular for AF2-1DA and intergranular for INCO 718.

  3. The Da Vinci European BioBank: A Metabolomics-Driven Infrastructure

    PubMed Central

    Carotenuto, Dario; Luchinat, Claudio; Marcon, Giordana; Rosato, Antonio; Turano, Paola

    2015-01-01

    We present here the organization of the recently-constituted da Vinci European BioBank (daVEB, https://www.davincieuropeanbiobank.org/it). The biobank was created as an infrastructure to support the activities of the Fiorgen Foundation (http://www.fiorgen.net/), a nonprofit organization that promotes research in the field of pharmacogenomics and personalized medicine. The way operating procedures concerning samples and data have been developed at daVEB largely stems from the strong metabolomics connotation of Fiorgen and from the involvement of the scientific collaborators of the foundation in international/European projects aimed to tackle the standardization of pre-analytical procedures and the promotion of data standards in metabolomics. PMID:25913579

  4. Evolution of the ATLAS PanDA Production and Distributed Analysis System

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Wenaus, T.; Nilsson, P.; Walker, R.; Stradling, A.; Fine, V.; Potekhin, M.; Panitkin, S.; Compostella, G.

    2012-12-01

    The PanDA (Production and Distributed Analysis) system has been developed to meet ATLAS production and analysis requirements for a data-driven workload management system capable of operating at LHC data processing scale. PanDA has performed well with high reliability and robustness during the two years of LHC data-taking, while being actively evolved to meet the rapidly changing requirements for analysis use cases. We will present an overview of system evolution including automatic rebrokerage and reattempt for analysis jobs, adaptation for the CernVM File System, support for the multi-cloud model through which Tier-2 sites act as members of multiple clouds, pledged resource management and preferential brokerage, and monitoring improvements. We will also describe results from the analysis of two years of PanDA usage statistics, current issues, and plans for the future.

  5. Detection of trace helium in G104 - 27, a 26,000 K DA white dwarf

    NASA Technical Reports Server (NTRS)

    Holberg, J. B.; Kidder, K. M.; Wesemael, F.

    1990-01-01

    The detection of the He I 4471 A feature in the moderately hot DA white dwarf G104 - 27 places this star within the rather sparse class of DAB white dwarfs. It does not appear possible, on the basis of the present data, to distinguish between a stratified and a homogeneously mixed atmosphere. While a stratified structure would be natural for a DA white dwarf, G104 - 27 resides near the red edge of the DB gap, in a region where DA stars could transform into DB stars through convective mixing in the underlying He envelope and thereby destroy envelope stratification. Both atmospheres are consistent with the existing soft X-ray flux limit for this star.

  6. Fluorescence spectroscopy coupled with PARAFAC and PLS DA for characterization and classification of honey.

    PubMed

    Lenhardt, Lea; Bro, Rasmus; Zeković, Ivana; Dramićanin, Tatjana; Dramićanin, Miroslav D

    2015-05-15

    Fluorescence spectroscopy coupled with parallel factor analysis (PARAFAC) and Partial least squares Discriminant Analysis (PLS DA) were used for characterization and classification of honey. Excitation emission spectra were obtained for 95 honey samples of different botanical origin (acacia, sunflower, linden, meadow, and fake honey) by recording emission from 270 to 640 nm with excitation in the range of 240-500 nm. The number of fluorophores present in honey, excitation and emission spectra of each fluorophore, and their relative concentration are determined using a six-component PARAFAC model. Emissions from phenolic compounds and Maillard reaction products exhibited the largest difference among classes of honey of different botanical origin. The PLS DA classification model, constructed from PARAFAC model scores, detected fake honey samples with 100% sensitivity and specificity. Honey samples were also classified using PLS DA with errors of 0.5% for linden, 10% for acacia, and about 20% for both sunflower and meadow mix. PMID:25577082

  7. gLExec Integration with the ATLAS PanDA Workload Management System

    NASA Astrophysics Data System (ADS)

    Karavakis, E.; Barreiro, F.; Campana, S.; De, K.; Di Girolamo, A.; Litmaath, M.; Maeno, T.; Medrano, R.; Nilsson, P.; Wenaus, T.

    2015-12-01

    ATLAS user jobs are executed on Worker Nodes (WNs) by pilots sent to sites by pilot factories. This paradigm serves to allow a high job reliability and although it has clear advantages, such as making the working environment homogeneous, the approach presents security and traceability challenges. To address these challenges, gLExec can be used to let the payloads for each user be executed under a different UNIX user id that uniquely identifies the ATLAS user. This paper describes the recent improvements and evolution of the security model within the ATLAS PanDA system, including improvements in the PanDA pilot, in the PanDA server and their integration with MyProxy, a credential caching system that entitles a person or a service to act in the name of the issuer of the credential. Finally, it presents results from ATLAS user jobs running with gLExec and describes the deployment campaign within ATLAS.

  8. Chronic 5-HT transporter blockade reduces DA signaling to elicit basal ganglia dysfunction.

    PubMed

    Morelli, Emanuela; Moore, Holly; Rebello, Tahilia J; Gray, Neil; Steele, Kelly; Esposito, Ennio; Gingrich, Jay A; Ansorge, Mark S

    2011-11-01

    Serotonin (5-HT)-selective reuptake inhibitors (SSRIs) are widely administered for the treatment of depression, anxiety, and other neuropsychiatric disorders, but response rates are low, and side effects often lead to discontinuation. Side effect profiles suggest that SSRIs inhibit dopaminergic activity, but mechanistic insight remains scarce. Here we show that in mice, chronic 5-HT transporter (5-HTT) blockade during adulthood but not during development impairs basal ganglia-dependent behaviors in a dose-dependent and reversible fashion. Furthermore, chronic 5-HTT blockade reduces striatal dopamine (DA) content and metabolism. A causal relationship between reduced DA signaling and impaired basal ganglia-dependent behavior is indicated by the reversal of behavioral deficits through L-DOPA administration. Our data suggest that augmentation of DA signaling would reduce side effects and increase efficacies of SSRI-based therapy. PMID:22049417

  9. The coolest DA white dwarfs detected at soft X-ray wavelengths

    NASA Technical Reports Server (NTRS)

    Kidder, K. M.; Holberg, J. B.; Barstow, M. A.; Tweedy, R. W.; Wesemael, F.

    1992-01-01

    New soft X-ray/EUV photometric observations of the DA white dwarfs KPD 0631 + 1043 = WD 0631 + 107 and PG 1113 + 413 = WD 1113 + 413 are analyzed. Previously reported soft X-ray detections of three other DAs and the failure to detect a fourth DA in deep Exosat observations are investigated. New ground-based spectra are presented for all of the objects, with IUE Ly-alpha spectra for some. These data are used to constrain the effective temperatures and surface gravities. The improved estimates of these parameters are employed to refer a photospheric He abundance for the hotter objects and to elucidate an effective observational low-temperature threshold for the detection of pure hydrogen DA white dwarfs at soft X-ray wavelengths.

  10. Integration of PanDA workload management system with Titan supercomputer at OLCF

    NASA Astrophysics Data System (ADS)

    De, K.; Klimentov, A.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.

    2015-12-01

    The PanDA (Production and Distributed Analysis) workload management system (WMS) was developed to meet the scale and complexity of LHC distributed computing for the ATLAS experiment. While PanDA currently distributes jobs to more than 100,000 cores at well over 100 Grid sites, the future LHC data taking runs will require more resources than Grid computing can possibly provide. To alleviate these challenges, ATLAS is engaged in an ambitious program to expand the current computing model to include additional resources such as the opportunistic use of supercomputers. We will describe a project aimed at integration of PanDA WMS with Titan supercomputer at Oak Ridge Leadership Computing Facility (OLCF). The current approach utilizes a modified PanDA pilot framework for job submission to Titan's batch queues and local data management, with light-weight MPI wrappers to run single threaded workloads in parallel on Titan's multicore worker nodes. It also gives PanDA new capability to collect, in real time, information about unused worker nodes on Titan, which allows precise definition of the size and duration of jobs submitted to Titan according to available free resources. This capability significantly reduces PanDA job wait time while improving Titan's utilization efficiency. This implementation was tested with a variety of Monte-Carlo workloads on Titan and is being tested on several other supercomputing platforms. Notice: This manuscript has been authored, by employees of Brookhaven Science Associates, LLC under Contract No. DE-AC02-98CH10886 with the U.S. Department of Energy. The publisher by accepting the manuscript for publication acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes.

  11. Characterization of the 70 kDa polypeptide of the Na/Ca exchanger.

    PubMed Central

    Saba, R I; Bollen, A; Herchuelz, A

    1999-01-01

    The Na/Ca exchanger is associated with 160, 120 and 70 kDa polypeptides whose nature is poorly understood. We have purified and characterized the Na/Ca exchanger from bovine cardiac sarcolemmal vesicles (SLVs) by using ion-exchange and affinity chromatographies. The Na/Ca exchanger-enriched fraction was reconstituted into asolectin liposomes [lipid to protein ratio 10:1 (w/w)] that showed Na/Ca exchange activity. Under non-reducing conditions, SDS/PAGE showed a single 70 kDa polypeptide, which was further characterized by immunoblots with different antibodies: SWant, raised against the purified exchanger protein; NH2-terminus, residues 1-21; NCX1, residues 393-406; and Exon F, residues 622-644. Immunoblots under reducing conditions with SWant, NH2-terminus and NCX1 showed three bands migrating at 160, 120 and 70 kDa for SLV preparations, whereas Exon F reacted only with the 160 and 120 kDa bands. Under non-reducing conditions, immunoblots with purified reconstituted Na/Ca exchanger showed a single band at 70 kDa reacting with SWant, NH2-terminus and NCX1 but not with Exon F. We conclude that the 70 kDa protein is associated with Na/Ca exchange activity, has the same N-terminal sequence as the cloned bovine cardiac exchanger, and has its length decreased by at least 35% from its C-terminal portion as compared with that of the wild-type exchanger. PMID:9931309

  12. Recurring X-ray outbursts in the supernova impostor SN 2010da in NGC 300

    NASA Astrophysics Data System (ADS)

    Binder, B.; Williams, B. F.; Kong, A. K. H.; Gaetz, T. J.; Plucinsky, P. P.; Skillman, E. D.; Dolphin, A.

    2016-04-01

    We present new observations of the `supernova impostor' SN 2010da using the Chandra X-ray Observatory and the Hubble Space Telescope. During the initial 2010 outburst, the 0.3-10 keV luminosity was observed by Swift to be ˜5 × 1038 erg s-1 and faded by a factor of ˜25 in a four month period. Our two new Chandra observations show a factor of ˜10 increase in the 0.35-8 keV X-ray luminosity, from ˜4 × 1036 to 4 × 1037 erg s-1 in ˜6 months, and the X-ray spectrum is consistent in both observations with a power-law with a photon index of Γ ˜ 0. We find evidence of X-ray spectral state changes: when SN 2010da is in a high-luminosity state, the X-ray spectrum is harder (Γ ˜0) compared to the low-luminosity state (Γ ˜ 1.2 ± 0.8). Using our Hubble observations, we fit the colour-magnitude diagram of the coeval stellar population to estimate a time since formation of the SN 2010da progenitor system of ≲5 Myr. Our observations are consistent with SN 2010da being a high-mass X-ray binary (HMXB) composed of a neutron star and a luminous blue variable-like companion, although we cannot rule out the possibility that SN 2010da is an unusually X-ray bright massive star. The ≲5 Myr age is consistent with the theoretically predicted delay time between the formation of a massive binary and the onset of the HMXB phase. It is possible that the initial 2010 outburst marked the beginning of X-ray production in the system, making SN 2010da possibly the first massive progenitor binary ever observed to evolve into an HMXB.

  13. [Euclides da Cunha in the Amazon: historical discontinuities in how the forest is seen and narrated].

    PubMed

    Guimarães, Leandro Belinaso

    2010-01-01

    The article examines how the discursive, social, and political processes that nationalized the Amazon gained firm footing during Brazil's First Republic. It is argued that Euclides da Cunha's texts on the Amazon see the forest and narrate its story in a way that differs from the travel accounts of nineteenth-century naturalists, above all foreign ones. Drawing inspiration from Latin American cultural studies, the article reinforces the argument that there is a historical discontinuity between the romantic foreign travel literature of the nineteenth century and the early twentieth-century writings of Euclides da Cunha. PMID:21461535

  14. Aerodynamic design of the Cal Poly Da Vinci Human-Powered Helicopter

    NASA Technical Reports Server (NTRS)

    Larwood, Scott; Saiki, Neal

    1990-01-01

    This paper will discuss the methodology used in designing the rotor and drive propellers for the third generation Cal Poly Da Vinci Human-Powered Helicopter. The rotor was designed using a lifting surface, uniform inflow hover analysis code and the propeller was designed using a minimum induced-loss method. Construction, geometry, and operating considerations are discussed as they impact the designs. Optimization of the design performance is also explained. The propellers were tested in a wind tunnel and results are compared with theoretical data. Successful flight tests of the Da Vinci III are discussed.

  15. Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil

    PubMed Central

    Song, Ok-Ryul; Lee, Seung-Jin; Lee, Yong-Seok; Lee, Sang-Cheol; Kim, Keun-Ki; Choi, Yong-Lark

    2008-01-01

    A mineral phosphate solubilizing bacterium, Burkholderia cepacia DA23 has been isolated from cultivated soils. Phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. When 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to the pH drop by the strain. Analysis of the culture medium by high pressure liquid chromatography identified gluconic acid as the main organic acid released by Burkholderia cepacia DA23. Gluconic acid production was apparently the result of the glucose dehydrogenase activity and glucose dehydrogenase was affected by phosphate regulation. PMID:24031195

  16. Spectroscopy of the DA white dwarfs - Automatic atmospheric parameterization and mass distribution

    NASA Technical Reports Server (NTRS)

    Mcmahan, Robert K.

    1989-01-01

    A method for the automatic calculation of the atmospheric parameters (Teff and log g) of hydrogen-rich degenerate stars from low-resolution spectra is described, and then applied to the spectra of 53 DA white dwarfs. A value for the width of the DA mass distribution of sigma M/solar-M not greater than +0.10 is obtained using the proposed approach. The data indicate that the distribution is asymmetrically skewed to low masses; however, there is also evidence of a high-mass non-Gaussian tail.

  17. The prototypical 4.1R-10-kDa domain and the 4.1g-10-kDa paralog mediate fodrin-actin complex formation.

    PubMed

    Kontrogianni-Konstantopoulos, A; Frye, C S; Benz, E J; Huang, S C

    2001-06-01

    A complex family of 4.1R isoforms has been identified in non-erythroid tissues. In this study we characterized the exonic composition of brain 4.1R-10-kDa or spectrin/actin binding (SAB) domain and identified the minimal sequences required to stimulate fodrin/F-actin association. Adult rat brain expresses predominantly 4.1R mRNAs that carry an extended SAB, consisting of the alternative exons 14/15/16 and part of the constitutive exon 17. Exon 16 along with sequences carried by exon 17 is necessary and sufficient to induce formation of fodrin-actin-4.1R ternary complexes. The ability of the respective SAB domains of 4.1 homologs to sediment fodrin/actin was also investigated. 4.1G-SAB stimulates association of fodrin/actin, although with an approximately 2-fold reduced efficiency compared with 4.1R-10-kDa, whereas 4.1N and 4.1B do not. Sequencing of the corresponding domains revealed that 4.1G-SAB carries a cassette that shares significant homology with 4.1R exon 16, whereas the respective sequence is divergent in 4.1N and absent from brain 4.1B. An approximately 150-kDa 4.1R and an approximately 160-kDa 4.1G isoforms are present in PC12 lysates that occur in vivo in a supramolecular complex with fodrin and F-actin. Moreover, proteins 4.1R and 4.1G are distributed underneath the plasma membrane in PC12 cells. Collectively, these observations suggest that brain 4.1R and 4.1G may modulate the membrane mechanical properties of neuronal cells by promoting fodrin/actin association. PMID:11274145

  18. Detection and differentiation of 22 kDa and 20 kDa Growth Hormone proteoforms in human plasma by LC-MS/MS.

    PubMed

    Such-Sanmartín, Gerard; Bache, Nicolai; Bosch, Jaume; Gutiérrez-Gallego, Ricardo; Segura, Jordi; Jensen, Ole N

    2015-04-01

    Human growth hormone (GH) is suspected to be widely and illegally used in sport to improve athletes' performance. For the detection of GH abuse, blood samples are screened for abnormal ratios between the 22 and 20 kDa GH proteoforms that demonstrate the administration of the synthetic hormone. Current detection methods are based on classical immunoassays as they provide sufficient sensitivity for the detection of GH proteoforms. These antibody based methods, however, suffer from unclear selectivity and potential cross-reactivity towards similar proteins. For unambiguous GH detection, we report a Mass Spectrometry ImmunoAssay (MSIA) that first enriches GH from plasma with an antibody of relatively low specificity, and subsequently quantifies the 22 and 20 kDa proteoforms by Selected Reaction Monitoring (SRM) LC-MS/MS analysis. This method proved superior to an antibody-free strategy based on GH purification by protein precipitation. Using GH-MSIA we successfully quantified the 22/20 kDa GH ratio in post-exercise capillary plasma extracted from two individuals. This GH-MSIA is applicable to anti-doping and GH-related disease analysis. PMID:25556001

  19. Análise da aplicação e dos resultados do modelo OPM3® para a área da saúde

    PubMed Central

    Augusto dos Santos, Luis; de Fátima Marin, Heimar

    2015-01-01

    Esta pesquisa procurou analisar se um modelo de questionário criado por uma comunidade internacional de gerenciamento de projetos e se é aplicavél a organizações voltadas a área de saúde. O modelo OPM3® (Organizational Project Management Maturity Model) foi criado para que organizações de qualquer área ou porte pudessem identificar a presença, ou ausência, de boas práticas de gerenciamento. O objetivo da aplicação desse modelo é avaliar sempre a organização e não o entrevistado. No presente artigo, são apresentados os resultados da aplicação desse modelo em uma organização que possuía produtos e serviços de tecnologia da informação aplicados à área de saúde. Este estudo verificou que o modelo é aplicável de forma rápida e que a organização analisada possuía um número expressivo de boas práticas. PMID:26924862

  20. 76 FR 56455 - DaVita, Inc.; Analysis of Agreement Containing Consent Orders To Aid Public Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-13

    ... terms of the Consent Agreement, DaVita is required to divest 28 dialysis clinics and terminate one... Commission Act, as amended, 15 U.S.C. 45, by lessening competition for the provision of outpatient dialysis... provider of outpatient dialysis services in the United States. DaVita operates 1,612 outpatient...

  1. The 37/67kDa laminin receptor (LR) inhibitor, NSC47924, affects 37/67kDa LR cell surface localization and interaction with the cellular prion protein

    PubMed Central

    Sarnataro, Daniela; Pepe, Anna; Altamura, Gennaro; De Simone, Imma; Pesapane, Ada; Nitsch, Lucio; Montuori, Nunzia; Lavecchia, Antonio; Zurzolo, Chiara

    2016-01-01

    The 37/67 kDa laminin receptor (LR) is a non-integrin protein, which binds both laminin-1 of the extracellular matrix and prion proteins, that hold a central role in prion diseases. The 37/67 kDa LR has been identified as interactor for the prion protein (PrPC) and to be required for pathological PrP (PrPSc) propagation in scrapie-infected neuronal cells, leading to the possibility that 37/67 kDa LR-PrPC interaction is related to the pathogenesis of prion diseases. A relationship between 37/67 kDa LR and PrPC in the presence of specific LR inhibitor compounds has not been investigated yet. We have characterized the trafficking of 37/67 kDa LR in both neuronal and non-neuronal cells, finding the receptor on the cell surface and nuclei, and identified the 67 kDa LR as the almost exclusive isoform interacting with PrPC. Here, we show that the treatment with the 37/67 kDa LR inhibitor, NSC47924, affects both the direct 37/67 kDa LR-PrPC interaction in vitro and the formation of the immunocomplex in live cells, inducing a progressive internalization of 37/67 kDa LR and stabilization of PrPC on the cell surface. These data reveal NSC47924 as a useful tool to regulate PrPC and 37/67 kDa LR trafficking and degradation, representing a novel small molecule to be tested against prion diseases. PMID:27071549

  2. Effect of DA-9701 on the Normal Motility and Clonidine-induced Hypomotility of the Gastric Antrum in Rats

    PubMed Central

    Kang, Je Wook; Han, Dae Kyeong; Kim, Ock Nyun; Lee, Kwang Jae

    2016-01-01

    Background/Aims DA-9701 is a novel prokinetic agent. In the present study, we investigated the effect of DA-9701 on the motility of the gastric antrum in the normal and clonidine-induced hypomotility in an in vivo animal model. Methods A strain gauge force transducer was sutured on the gastric antrum to measure the contractile activity in rats. A total of 28 rats were subclassified into the 4 groups: (1) the placebo group, (2) the DA-9701 group, (3) the placebo group in the clonidine-pretreated rats, and (4) the DA-9701 group in the clonidine-pretreated rats. After the basal recording, either placebo (3% [w/v] hydroxypropylmethyl cellulose) or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. In the clonidine-pretreated rats, either placebo or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. Results Oral administration of DA-9701 did not significantly alter the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the placebo group. The administration of clonidine decreased the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the administration of placebo. This reduction of the antral motility by the administration of clonidine was not observed in the clonidine-pretreated DA-9701 group. The percentage of the motility index in the postprandial period was significantly greater in the clonidine-pretreated DA-9701 group, compared with the clonidine-pretreated placebo group. Conclusions DA-9701 improves the hypomotility of the gastric antrum induced by clonidine, suggesting its gastroprokinetic effect in the pathologic condition. PMID:26755679

  3. 40 CFR 60.43Da - Standards for sulfur dioxide (SO2).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Standards for sulfur dioxide (SO2). 60... Steam Generating Units § 60.43Da Standards for sulfur dioxide (SO2). (a) On and after the date on which... the percent reduction requirement is determined on a 24-hour basis. (d) Sulfur dioxide emissions...

  4. 40 CFR 60.43Da - Standards for sulfur dioxide (SO2).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Standards for sulfur dioxide (SO2). 60... Steam Generating Units § 60.43Da Standards for sulfur dioxide (SO2). (a) On and after the date on which... the percent reduction requirement is determined on a 24-hour basis. (d) Sulfur dioxide emissions...

  5. 40 CFR 60.43Da - Standards for sulfur dioxide (SO2).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Standards for sulfur dioxide (SO2). 60... Steam Generating Units § 60.43Da Standards for sulfur dioxide (SO2). (a) On and after the date on which... the percent reduction requirement is determined on a 24-hour basis. (d) Sulfur dioxide emissions...

  6. MATERNAL ATRAZINE (ATR) ALTERS HYPOTHALAMIC DOPAMINE (HYP-DA) AND SERUM PROLACTIN (SPRL) IN MALE PUPS

    EPA Science Inventory

    Maternal Atrazine (ATR) alters hypothalamic dopamine (HYP-DA) and serum prolactin (sPRL) in male pups. 1Christopher Langdale, 2Tammy Stoker and 2Ralph Cooper. 1 Dept. of Cell Biology, North Carolina State University College of Veterinary Medicine, Raleigh, NC. 2 Endocrinology ...

  7. In response to Dr. Jose AP da Silva: fall prevention with vitamin D clarifications needed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We agree with Dr. da Silva, and wish to clarify three issues raised by the recent IOM report concerning our 2009 meta-analysis on vitamin D and fall prevention. 1. The IOM questioned the inclusion of Broe et al., which did not pre-specify falls as a primary or secondary outcome. While this did viol...

  8. GS32, a novel Golgi SNARE of 32 kDa, interacts preferentially with syntaxin 6.

    PubMed

    Wong, S H; Xu, Y; Zhang, T; Griffiths, G; Lowe, S L; Subramaniam, V N; Seow, K T; Hong, W

    1999-01-01

    Syntaxin 1, synaptobrevins or vesicle-associated membrane proteins, and the synaptosome-associated protein of 25 kDa (SNAP-25) are key molecules involved in the docking and fusion of synaptic vesicles with the presynaptic membrane. We report here the molecular, cell biological, and biochemical characterization of a 32-kDa protein homologous to both SNAP-25 (20% amino acid sequence identity) and the recently identified SNAP-23 (19% amino acid sequence identity). Northern blot analysis shows that the mRNA for this protein is widely expressed. Polyclonal antibodies against this protein detect a 32-kDa protein present in both cytosol and membrane fractions. The membrane-bound form of this protein is revealed to be primarily localized to the Golgi apparatus by indirect immunofluorescence microscopy, a finding that is further established by electron microscopy immunogold labeling showing that this protein is present in tubular-vesicular structures of the Golgi apparatus. Biochemical characterizations establish that this protein behaves like a SNAP receptor and is thus named Golgi SNARE of 32 kDa (GS32). GS32 in the Golgi extract is preferentially retained by the immobilized GST-syntaxin 6 fusion protein. The coimmunoprecipitation of syntaxin 6 but not syntaxin 5 or GS28 from the Golgi extract by antibodies against GS32 further sustains the preferential interaction of GS32 with Golgi syntaxin 6. PMID:9880331

  9. Tumour-infiltrating lymphocytes bear the 75 kDa tumour necrosis factor receptor.

    PubMed Central

    Trentin, L.; Zambello, R.; Bulian, P.; Cerutti, A.; Enthammer, C.; Cassatella, M.; Nitti, D.; Lise, M.; Agostini, C.; Semenzato, G.

    1995-01-01

    Tumour necrosis factor alpha (TNF-alpha) is a cytokine with a variety of immunological properties. The identification of two receptors for this molecule, i.e. the 75 kDa and the 55 kDa TNF receptors (TNF-R), recently clarified the mechanisms through which this cytokine provides its wide range of immunomodulatory activities. In this study we have investigated the expression and the functional properties of these receptors on tumour-infiltrating lymphocytes (TILs) recovered from 17 patients with solid cancers (melanoma, colorectal carcinoma and lung cancer). To this end, TIL lines and freshly isolated TILs were evaluated for (a) the expression and the functional role of TNF receptors following culture in the presence of interleukin 2 (IL-2) and (b) the production of TNF-alpha following culture with IL-2 and the role of this cytokine in IL-2-driven TIL proliferation. Flow cytometry analysis demonstrated that TILs bear the 75 kDa TNF-R. Moreover, TIL lines express detectable messages for TNF-alpha and release this cytokine. Functional in vitro studies have shown that anti-TNF-alpha, as well as anti-75 kDa TNF-R antibodies, are able to inhibit the IL-2-induced TIL proliferation. These data demonstrate that TILs are equipped with a fully functional TNF-R system and suggest a putative role for this receptor and its ligand in the activation and expression of TILs following immunotherapy with IL-2. Images Figure 2 PMID:7841036

  10. Translocation of an 89-kDa periplasmic protein is associated with Holospora infection

    SciTech Connect

    Iwatani, Koichi; Dohra, Hideo; Lang, B. Franz; Burger, Gertraud; Hori, Manabu; Fujishima, Masahiro . E-mail: fujishim@yamaguchi-u.ac.jp

    2005-12-02

    The symbiotic bacterium Holospora obtusa infects the macronucleus of the ciliate Paramecium caudatum. After ingestion by its host, an infectious form of Holospora with an electron-translucent tip passes through the host digestive vacuole and penetrates the macronuclear envelope with this tip. To investigate the underlying molecular mechanism of this process, we raised a monoclonal antibody against the tip-specific 89-kDa protein, sequenced this partially, and identified the corresponding complete gene. The deduced protein sequence carries two actin-binding motifs. Indirect immunofluorescence microscopy shows that during escape from the host digestive vacuole, the 89-kDa proteins translocates from the inside to the outside of the tip. When the bacterium invades the macronucleus, the 89-kDa protein is left behind at the entry point of the nuclear envelope. Transmission electron microscopy shows the formation of fine fibrous structures that co-localize with the antibody-labeled regions of the bacterium. Our findings suggest that the 89-kDa protein plays a role in Holospora's escape from the host digestive vacuole, the migration through the host cytoplasm, and the invasion into the macronucleus.