Science.gov

Sample records for da norma sa

  1. Análise da aplicação e dos resultados do modelo OPM3® para a área da saúde

    PubMed Central

    Augusto dos Santos, Luis; de Fátima Marin, Heimar

    2015-01-01

    Esta pesquisa procurou analisar se um modelo de questionário criado por uma comunidade internacional de gerenciamento de projetos e se é aplicavél a organizações voltadas a área de saúde. O modelo OPM3® (Organizational Project Management Maturity Model) foi criado para que organizações de qualquer área ou porte pudessem identificar a presença, ou ausência, de boas práticas de gerenciamento. O objetivo da aplicação desse modelo é avaliar sempre a organização e não o entrevistado. No presente artigo, são apresentados os resultados da aplicação desse modelo em uma organização que possuía produtos e serviços de tecnologia da informação aplicados à área de saúde. Este estudo verificou que o modelo é aplicável de forma rápida e que a organização analisada possuía um número expressivo de boas práticas. PMID:26924862

  2. E-Interview: Norma Fox Mazer.

    ERIC Educational Resources Information Center

    Angel, Ann

    2001-01-01

    Presents an interview with Norma Fox Mazer, a writer of children's books. Describes how she creates a story. Discusses how writing a story, whether a short story or a novel, is an intricate balance of character, event, and voice. (SG)

  3. Norma Gray: Leading the Way for Children.

    ERIC Educational Resources Information Center

    Casto, James E.

    1999-01-01

    Founded by Norma Gray in 1972 as an early-childhood demonstration center, River Valley Child Development Services now operates various programs related to young children in nine southern West Virginia counties and a statewide apprenticeship program for child-development specialists. Programs provide child care, after-school programs,…

  4. Teaching Labor Relations with "Norma Rae"

    ERIC Educational Resources Information Center

    Taylor, Vicki Fairbanks; Provitera, Michael J.

    2011-01-01

    Undergraduate business students in North America are often unfamiliar with the labor organizing process and frequently fail to identify with the reasons why workers join unions. This article suggests a discussion exercise based on the 1979 film, "Norma Rae," by 20th Century Fox, as an effective tool for familiarizing students with fundamental…

  5. SaDA: From Sampling to Data Analysis-An Extensible Open Source Infrastructure for Rapid, Robust and Automated Management and Analysis of Modern Ecological High-Throughput Microarray Data.

    PubMed

    Singh, Kumar Saurabh; Thual, Dominique; Spurio, Roberto; Cannata, Nicola

    2015-06-01

    One of the most crucial characteristics of day-to-day laboratory information management is the collection, storage and retrieval of information about research subjects and environmental or biomedical samples. An efficient link between sample data and experimental results is absolutely important for the successful outcome of a collaborative project. Currently available software solutions are largely limited to large scale, expensive commercial Laboratory Information Management Systems (LIMS). Acquiring such LIMS indeed can bring laboratory information management to a higher level, but most of the times this requires a sufficient investment of money, time and technical efforts. There is a clear need for a light weighted open source system which can easily be managed on local servers and handled by individual researchers. Here we present a software named SaDA for storing, retrieving and analyzing data originated from microorganism monitoring experiments. SaDA is fully integrated in the management of environmental samples, oligonucleotide sequences, microarray data and the subsequent downstream analysis procedures. It is simple and generic software, and can be extended and customized for various environmental and biomedical studies. PMID:26047146

  6. SaDA: From Sampling to Data Analysis—An Extensible Open Source Infrastructure for Rapid, Robust and Automated Management and Analysis of Modern Ecological High-Throughput Microarray Data

    PubMed Central

    Singh, Kumar Saurabh; Thual, Dominique; Spurio, Roberto; Cannata, Nicola

    2015-01-01

    One of the most crucial characteristics of day-to-day laboratory information management is the collection, storage and retrieval of information about research subjects and environmental or biomedical samples. An efficient link between sample data and experimental results is absolutely important for the successful outcome of a collaborative project. Currently available software solutions are largely limited to large scale, expensive commercial Laboratory Information Management Systems (LIMS). Acquiring such LIMS indeed can bring laboratory information management to a higher level, but most of the times this requires a sufficient investment of money, time and technical efforts. There is a clear need for a light weighted open source system which can easily be managed on local servers and handled by individual researchers. Here we present a software named SaDA for storing, retrieving and analyzing data originated from microorganism monitoring experiments. SaDA is fully integrated in the management of environmental samples, oligonucleotide sequences, microarray data and the subsequent downstream analysis procedures. It is simple and generic software, and can be extended and customized for various environmental and biomedical studies. PMID:26047146

  7. V390 Nor = Nova Normae 2007

    NASA Astrophysics Data System (ADS)

    Waagen, Elizabeth O.

    2007-06-01

    Nova Normae 2007 was discovered photographically by William Liller on June 15.086 UT at magnitude 9.4. Precise position measured by G. Bolt from his unfiltered CCD image of June 16.7 UT: 16:32:11.51 -45:09:13.4 (2000.0). Giorgio Di Scala reported to the AAVSO that a low-resolution spectrum indicates a nova a week or so after outburst, with strong H-alpha emission. E. Kazarovets, Sternberg Astronomical Institute, reports that N Nor 07 has been assigned the name V390 Nor. Discovery originally announced in IAU Central Bureau Electronic Telegram 982 (Daniel W. E. Green) and AAVSO Special Notice #49 (Arne Henden). Information in this Alert Notice was received at AAVSO from William Liller, Giorgio Di Scala, or via IAU Circular No. 8850, ed. Daniel W. E. Green. A chart for V390 Nor is available via the Variable Star Plotter (VSP). Go to: http://www.aavso.org/observing/charts/vsp/ and enter the name V390 NOR.

  8. Low Mass Star Formation in the Norma Cloud

    NASA Astrophysics Data System (ADS)

    Reipurth, B.; Nielbock, M.

    2008-12-01

    A small filamentary cloud in Norma hosts a number of young low-mass stars in various stages of evolution, from visible Hα emission stars to embedded sources detected only in the sub-millimeter regime. The best known source is V346 Nor, an FU Orionis star that brightened in the early 1980s. The morphology of the cloud complex and an apparent age gradient along the cloud suggests that star formation in this region was triggered by an external event.

  9. Location of the Norma transient with the HEAO 1 scanning modulation collimator. [X ray source in Norma Constellation

    NASA Technical Reports Server (NTRS)

    Fabbiano, G.; Gursky, H.; Schwartz, D. A.; Schwarz, J.; Bradt, H. V.; Doxsey, R. E.

    1978-01-01

    A precise position has been obtained for an X-ray transient source in Norma. The location uncertainty includes a variable star previously suggested to be the optical counterpart. This transient is associated with the steady X-ray source MX 1608-52 and probably with an X-ray burst source. A binary system containing a low-mass primary and a neutron-star or black-hole secondary of a few solar masses is consistent with the observations.

  10. Uhuru observations of the Norma X-ray burster

    NASA Technical Reports Server (NTRS)

    Grindlay, J.; Gursky, H.

    1976-01-01

    Four X-ray bursts consistent with a single source in Norma are reported which were discovered by reexamining Uhuru data obtained between 1970 and 1973. The temporal and spectral characteristics of the bursts are described and shown to be similar to those displayed by bursts from the globular cluster NGC 6224. An error box of the source location is given, and it is found that both the position and intensity of the four bursts are consistent with those of 10 bursts detected by the Vela satellites in 1976. It is concluded that the source is the same as that observed by the Vela and is an X-ray burster with characteristics similar to those of certain other bursters. XB 1608-52 is suggested as the designation of this burster, possible burst models are considered, and it is noted that the error box of the present source contains an identified globular cluster.

  11. 15. Historic American Buildings Survey Norma L. McAfee Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Historic American Buildings Survey Norma L. McAfee - Photographer National Park Service San Francisco, California Photo Taken: June 22, 1940 VIEW FROM SOUTHWEST - Custom House, Custom House Plaza, Monterey, Monterey County, CA

  12. 14. Historic American Buildings Survey Norma L. McAfee Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Historic American Buildings Survey Norma L. McAfee - Photographer National Park Service San Francisco, California Photo Taken: June 22, 1940 GENERAL VIEW - Custom House, Custom House Plaza, Monterey, Monterey County, CA

  13. 13. Historic American Buildings Survey Norma L. McAfee Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Historic American Buildings Survey Norma L. McAfee - Photographer National Park Service San Francisco, California Photo Taken: June 22, 1940 VIEW FROM SOUTHWEST - Custom House, Custom House Plaza, Monterey, Monterey County, CA

  14. Sandqvist 187 - A dense molecular cloud in Norma

    NASA Technical Reports Server (NTRS)

    Alvarez, H.; Bronfman, L.; Cohen, R.; Garay, G.; Graham, J.; Thaddeus, P.

    1986-01-01

    Observations of Sandqvist 187, an elongated dust cloud in the southern constellation Norma are presented and discussed. The cloud contains two Herbig-Haro objects, HH 56 and HH 57. HH 57 currently displays on its NE edge a 17th mag variable star of the FU Ori type. Using the Columbia University 1.2 m millimeter-wave telescope at Cerro Tololo, the region is mapped and an extended CO cloud which envelops and is elongated along the optical dust cloud is found. The position of maximum CO emission coincides with HH 56 and HH 57. Assuming a distance of 0.7 kpc, the total mass of the cloud is found to be close to 500 solar masses. The CO spectra show evidence of a molecular flow. Photographs and CCD images obtained mostly with the CTIO 4 m telescope show the detailed optical structure of the dark cloud's core region. The Herbig-Haro object HH 56 appears to be related to an emission-line star embedded in the small nebula Reipurth 13, not to the FU Ori star in HH 57.

  15. Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189

    PubMed Central

    Naz, Sehar Afshan; Jabeen, Nusrat; Sohail, Muhammad; Rasool, Sheikh Ajaz

    2015-01-01

    Abstract Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent. PMID:26691474

  16. A Receding Halo Sub-structure Towards Norma

    NASA Astrophysics Data System (ADS)

    Chakrabarti, Sukanya

    2016-01-01

    We present results from follow-up spectroscopic observations of clustered Cepheid candidates identified from K-band light curves towards the Norma constellation (Chakrabarti et al. 2015), as well as others that we have found more recently. The average radial velocity of these stars is ~ 200 km/s, which is large and distinct from that of the Galaxy's stellar disk. These objects at l ~ -27 and b ~ -1 are therefore halo stars; using the period-luminosity relation of Type I Cepheids, they are at ~ 90 kpc. While the spectra do not have sufficient S/N to independently determine the metallicity and spectral type of the stars, there is a clear correspondence between the observed Brackett series lines in these observations and in known Type I Cepheids. Distances determined from the K-band period-luminosity relation (Matsunaga et al. 2013) and the 3.6 μm period-luminosity relation (Scowcroft et al. 2011) agree closely, and I-band observations have confirmed the periods of these sources. The extinction corrected J - Ks colors of these sources are comparable to known Type I Cepheids (Persson et al. 2004). The observed radial velocity of these stars agrees with predictions from dynamical models (Chakrabarti & Blitz 2009). If these stars are indeed members of the predicted dark-matter dominated dwarf galaxy that perturbed the outer HI disk of the Milky Way, this would represent the first application of Galactoseismology. These observations also challenge models of the Galactic halo. Young Cepheid variables are unexpected in models of the Galactic halo, though star formation due to infall of gas-rich dwarf galaxies may well produce a small population of yet undiscovered Cepheids in the outer halo.

  17. The Norma arm region Chandra survey catalog: X-ray populations in the spiral arms

    SciTech Connect

    Fornasini, Francesca M.; Tomsick, John A.; Bodaghee, Arash; Krivonos, Roman A.; An, Hongjun; Rahoui, Farid; Gotthelf, Eric V.; Bauer, Franz E.; Stern, Daniel

    2014-12-01

    We present a catalog of 1415 X-ray sources identified in the Norma Arm Region Chandra Survey (NARCS), which covers a 2° × 0.°8 region in the direction of the Norma spiral arm to a depth of ≈20 ks. Of these sources, 1130 are point-like sources detected with ≥3σ confidence in at least one of three energy bands (0.5-10, 0.5-2, and 2-10 keV), five have extended emission, and the remainder are detected at low significance. Since most sources have too few counts to permit individual classification, they are divided into five spectral groups defined by their quantile properties. We analyze stacked spectra of X-ray sources within each group, in conjunction with their fluxes, variability, and infrared counterparts, to identify the dominant populations in our survey. We find that ∼50% of our sources are foreground sources located within 1-2 kpc, which is consistent with expectations from previous surveys. Approximately 20% of sources are likely located in the proximity of the Scutum-Crux and near Norma arm, while 30% are more distant, in the proximity of the far Norma arm or beyond. We argue that a mixture of magnetic and nonmagnetic cataclysmic variables dominates the Scutum-Crux and near Norma arms, while intermediate polars and high-mass stars (isolated or in binaries) dominate the far Norma arm. We also present the cumulative number count distribution for sources in our survey that are detected in the hard energy band. A population of very hard sources in the vicinity of the far Norma arm and active galactic nuclei dominate the hard X-ray emission down to f{sub X} ≈ 10{sup –14} erg cm{sup –2} s{sup –1}, but the distribution curve flattens at fainter fluxes. We find good agreement between the observed distribution and predictions based on other surveys.

  18. Using Norma Fox Mazer's "Out of Control" To Reach Kids Where They Hide.

    ERIC Educational Resources Information Center

    Bott, C J

    2001-01-01

    Discusses how one teacher uses Norma Fox Mazer's "Out of Control" to explore leaders and followers as the main topic of discussion in a sophomore class. Describes how students keep a reader's journal with quotations from the text and personal responses. (SG)

  19. Librarian of the Year 2008: New Jersey State Librarian Norma Blake

    ERIC Educational Resources Information Center

    Berry, John N., III

    2008-01-01

    This article profiles "Library Journal's" (LJ) chosen 2008 Librarian of the Year. Librarians and officials in education and government all recount the leadership and creativity brought to library service in New Jersey by State Librarian Norma Blake. She has sparked proactive, collaborative initiatives that have taken libraries of all types "out of…

  20. Creating Imaginative Worlds: Unique Details and Structure in Norma Fox Mazer's Young Adult Fiction.

    ERIC Educational Resources Information Center

    Angel, Ann

    2001-01-01

    Discusses how writer Norma Fox Mazer has helped many readers make the leap between reality and imagination simply in the way she handles details in the lives of her characters. Explores the ideas of communicating with detail, experimenting with structure, and playing with time in crucial scenes. (SG)

  1. Proceedings of the Nordic Conference on Mathematics Teaching (NORMA-94) Lahti 1994. Research Report 141.

    ERIC Educational Resources Information Center

    Pehkonen, Erkki, Ed.

    This report contains papers from the Nordic Conference on Mathematics Teaching (NORMA-94). The first three papers are plenary talks aimed at giving the participants an opportunity to form a coherent view of the new theories of learning. The themes of the paper sessions addressed a variety of topics on different levels from elementary school to…

  2. Number of SA Astronomy Researchers

    NASA Astrophysics Data System (ADS)

    Glass, I. S.

    2015-12-01

    The SA professional astronomical community has grown enormously in recent years with the advent of SALT, SKA/MeerKAT/KAT and HESS (Namibia). In this article I have made an attempt to list the people involved, namely those with doctorates working in fields of astronomy and related technologies, cosmic rays, cosmology and space science.

  3. Initial results from NuSTAR observations of the Norma Arm

    SciTech Connect

    Bodaghee, Arash; Tomsick, John A.; Krivonos, Roman; Barrière, Nicolas; Boggs, Steven E.; Craig, William W.; Stern, Daniel; Bauer, Franz E.; Fornasini, Francesca M.; Christensen, Finn E.; Gotthelf, Eric V.; Hailey, Charles J.; Mori, Kaya; Harrison, Fiona A.; Hong, Jaesub; Zhang, William W.

    2014-08-10

    Results are presented for an initial survey of the Norma Arm gathered with the focusing hard X-Ray Telescope NuSTAR. The survey covers 0.2 deg{sup 2} of sky area in the 3-79 keV range with a minimum and maximum raw depth of 15 ks and 135 ks, respectively. Besides a bright black-hole X-ray binary in outburst (4U 1630–47) and a new X-ray transient (NuSTAR J163433–473841), NuSTAR locates three sources from the Chandra survey of this region whose spectra are extended above 10 keV for the first time: CXOU J163329.5–473332, CXOU J163350.9–474638, and CXOU J163355.1–473804. Imaging, timing, and spectral data from a broad X-ray range (0.3-79 keV) are analyzed and interpreted with the aim of classifying these objects. CXOU J163329.5–473332 is either a cataclysmic variable or a faint low-mass X-ray binary. CXOU J163350.9–474638 varies in intensity on year-long timescales, and with no multi-wavelength counterpart, it could be a distant X-ray binary or possibly a magnetar. CXOU J163355.1–473804 features a helium-like iron line at 6.7 keV and is classified as a nearby cataclysmic variable. Additional surveys are planned for the Norma Arm and Galactic Center, and those NuSTAR observations will benefit from the lessons learned during this pilot study.

  4. Saturn I (SA-4) Launch

    NASA Technical Reports Server (NTRS)

    1963-01-01

    The Saturn I (SA-4) flight lifted off from Kennedy Space Center launch Complex 34, March 28, 1963. The fourth launch of Saturn launch vehicles developed at the Marshall Space Flight Center (MSFC), under the direction of Dr. Wernher von Braun, incorporated a Saturn I, Block I engine. The typical height of a Block I vehicle was approximately 163 feet and had only one live stage. It consisted of eight tanks, each 70 inches in diameter, clustered around a central tank, 105 inches in diameter. Four of the external tanks were fuel tanks for the RP-1 (kerosene) fuel. The other four, spaced alternately with the fuel tanks, were liquid oxygen tanks as was the large center tank. All fuel tanks and liquid oxygen tanks drained at the same rates respectively. The thrust for the stage came from eight H-1 engines, each producing a thrust of 165,000 pounds, for a total thrust of over 1,300,000 pounds. The engines were arranged in a double pattern. Four engines, located inboard, were fixed in a square pattern around the stage axis and canted outward slightly, while the remaining four engines were located outboard in a larger square pattern offset 40 degrees from the inner pattern. Unlike the inner engines, each outer engine was gimbaled. That is, each could be swung through an arc. They were gimbaled as a means of steering the rocket, by letting the instrumentation of the rocket correct any deviations of its powered trajectory. The block I required engine gimabling as the only method of guiding and stabilizing the rocket through the lower atmosphere. The upper stages of the Block I rocket reflected the three-stage configuration of the Saturn I vehicle. Like SA-3, the SA-4 flight's upper stage ejected 113,560 liters (30,000 gallons) of ballast water in the upper atmosphere for 'Project Highwater' physics experiment. Release of this vast quantity of water in a near-space environment marked the second purely scientific large-scale experiment. The SA-4 was the last Block I rocket

  5. Saturn I (SA-4) Launch

    NASA Technical Reports Server (NTRS)

    1963-01-01

    The Saturn I (SA-4) flight lifted off from Kennedy Space Center launch Complex 34, March 28, 1963. The fourth launch of Saturn launch vehicles, developed at the Marshall Space Flight Center (MSFC) under the direction of Dr. Wernher von Braun, incorporated a Saturn I, Block I engine. The typical height of a Block I vehicle was approximately 163 feet and had only one live stage. It consisted of eight tanks, each 70 inches in diameter, clustered around a central tank, 105 inches in diameter. Four of the external tanks were fuel tanks for the RP-1 (kerosene) fuel. The other four, spaced alternately with the fuel tanks, were liquid oxygen tanks as was the large center tank. All fuel tanks and liquid oxygen tanks drained at the same rates respectively. The thrust for the stage came from eight H-1 engines, each producing a thrust of 165,000 pounds, for a total thrust of over 1,300,000 pounds. The engines were arranged in a double pattern. Four engines, located inboard, were fixed in a square pattern around the stage axis and canted outward slightly, while the remaining four engines were located outboard in a larger square pattern offset 40 degrees from the inner pattern. Unlike the inner engines, each outer engine was gimbaled. That is, each could be swung through an arc. They were gimbaled as a means of steering the rocket, by letting the instrumentation of the rocket correct any deviations of its powered trajectory. The block I required engine gimabling as the only method of guiding and stabilizing the rocket through the lower atmosphere. The upper stages of the Block I rocket reflected the three-stage configuration of the Saturn I vehicle. Like SA-3, the SA-4 flight's upper stage ejected 113,560 liters (30,000 gallons) of ballast water in the upper atmosphere for 'Project Highwater' physics experiment. Release of this vast quantity of water in a near-space environment marked the second purely scientific large-scale experiment. The SA-4 was the last Block I rocket

  6. Preliminary treatment of bovine mastitis caused by Staphylococcus aureus, with trx-SA1, recombinant endolysin of S. aureus bacteriophage IME-SA1.

    PubMed

    Fan, Jindai; Zeng, Zhiliang; Mai, Kaijie; Yang, Yu; Feng, Jiaqi; Bai, Yang; Sun, Baoli; Xie, Qingmei; Tong, Yigang; Ma, Jingyun

    2016-08-15

    Methicillin-resistant Staphylococcus aureus (MRSA) has become a great threat to human and animal health and there is an urgent need to develop novel antibacterial agents to control this pathogen. The objective of this study was to obtain an active recombinant endolysin from the novel bacteriophage (IME-SA1), and conduct an efficacy trial of its effectiveness against bovine mastitis. We isolated a phage that was virulent and specific for S. aureus with an optimal multiplicity of infection of 0.01. Electron microscopy revealed that IME-SA1 was a member of the family Myoviridae, with an isometric head (98nm) and a long contractile tail (200nm). Experimental lysis experiments indicated the phage had an incubation period of 20min with a burst size of 80. When host bacteria were in early exponential growth stages, a multiplicity of infection of 0.01 resulted in a complete bacterial lysis after 9h. The endolysin gene (804bp) was cloned into the pET-32a bacterial expression vector and recombinant endolysin Trx-SA1 was successfully obtained with molecular size of about 47kDa. Preliminary results of therapeutic trials in cow udders showed that Trx-SA1 could effectively control mild clinical mastitis caused by S. aureus. The endolysin Trx-SA1 might be an alternative treatment strategy for infections caused by S. aureus, including MRSA. PMID:27374909

  7. Beyond SaGMRotI: Conversion to SaArb, SaSN, and SaMaxRot

    USGS Publications Warehouse

    Watson-Lamprey, J. A.; Boore, D.M.

    2007-01-01

    In the seismic design of structures, estimates of design forces are usually provided to the engineer in the form of elastic response spectra. Predictive equations for elastic response spectra are derived from empirical recordings of ground motion. The geometric mean of the two orthogonal horizontal components of motion is often used as the response value in these predictive equations, although it is not necessarily the most relevant estimate of forces within the structure. For some applications it is desirable to estimate the response value on a randomly chosen single component of ground motion, and in other applications the maximum response in a single direction is required. We give adjustment factors that allow converting the predictions of geometric-mean ground-motion predictions into either of these other two measures of seismic ground-motion intensity. In addition, we investigate the relation of the strike-normal component of ground motion to the maximum response values. We show that the strike-normal component of ground motion seldom corresponds to the maximum horizontal-component response value (in particular, at distances greater than about 3 km from faults), and that focusing on this case in exclusion of others can result in the underestimation of the maximum component. This research provides estimates of the maximum response value of a single component for all cases, not just near-fault strike-normal components. We provide modification factors that can be used to convert predictions of ground motions in terms of the geometric mean to the maximum spectral acceleration (SaMaxRot) and the random component of spectral acceleration (SaArb). Included are modification factors for both the mean and the aleatory standard deviation of the logarithm of the motions.

  8. Molecular Clouds and Massive Star Formation in the Norma Spiral Arm

    NASA Astrophysics Data System (ADS)

    García, P.; Bronfman, L.; May, J.

    2006-06-01

    The Norma spiral arm in the Southern Galaxy contains the most massive molecular clouds as well as the most FIR luminous regions of massive star formation in the Galactic disk. The tangent region of this arm, at a well defined distance of ≈ 4.5 kpc from the Sun, is ideal to study in detail the process of massive star formation in GMCs (Bronfman et al. 1988, ApJ, 324, 248). We present maps of the major GMCs in ^{12}CO and C^{18}O obtained with the Nanten 4-m telescope, at a resolution of 2.5 arcmin. We have obtained also CS (2-1) and CS(5-4) maps of several OB star formation regions embedded in these GMCs (Bronfman et al. 1996, A&AS, 115, 81). What is the contribution from embedded OB stars to the total FIR emission from these GMCs? What is the fraction of cloud molecular gas involved in massive star formation?

  9. Isolation of a haemorrhagic protein toxin (SA-HT) from the Indian venomous butterfish (Scatophagus argus, Linn) sting extract.

    PubMed

    Karmakar, S; Muhuri, D C; Dasgupta, S C; Nagchaudhuri, A K; Gomes, A

    2004-05-01

    A haemorrhagic protein toxin (SA-HT) was isolated and purified from the spine extract of the Indian venomous butterfish, S. argus Linn, by two step ion exchange chromatography. The toxin was homogeneous in native and SDS-PAGE gel. SDS-molecular weight of the toxin was found to be 18.1 +/- 0.09 kDa. SA-HT produced severe haemorrhage on stomach wall but devoid of cutaneous haemorrhage. UV, EDTA, trypsin, protease, cyproheptadine, indomethacin, acetylsalicylic acid and BW755C treatment significantly antagonized the haemorrhagic activity of SA-HT. The toxin produced dose and time dependent oedema on mice hind paw, which was significantly encountered by cyproheptadine, indomethacin and BW755C. SA-HT increased capillary permeability on guinea pig dorsal flank. On isolated guineapig ileum, rat fundus and uterus, SA-HT produced slow contraction which was completely antagonised by prostaglandin blocker SC19220. On isolated rat duodenum, SA-HT produced slow relaxation. SA-HT significantly increased plasma plasmin, serum MDA level and decreased serum SOD level indicating the possible involvement of cyclooxygenase and lipooxygenase pathway. PMID:15233468

  10. Saturn I (SA-3) Launch

    NASA Technical Reports Server (NTRS)

    1962-01-01

    The Saturn I (SA-3) flight lifted off from Kennedy Space Center launch Complex 34, November 16, 1962. The third launch of Saturn launch vehicles, developed at the Marshall Space Flight Center (MSFC) under the direction of Dr. Wernher von Braun, incorporated a Saturn I, Block I engine. The typical height of a Block I vehicle was approximately 163 feet. and had only one live stage. It consisted of eight tanks, each 70 inches in diameter, clustered around a central tank, 105 inches in diameter. Four of the external tanks were fuel tanks for the RP-1 (kerosene) fuel. The other four, spaced alternately with the fuel tanks, were liquid oxygen tanks as was the large center tank. All fuel tanks and liquid oxygen tanks drained at the same rates respectively. The thrust for the stage came from eight H-1 engines, each producing a thrust of 165,000 pounds, for a total thrust of over 1,300,000 pounds. The engines were arranged in a double pattern. Four engines, located inboard, were fixed in a square pattern around the stage axis and canted outward slightly, while the remaining four engines were located outboard in a larger square pattern offset 40 degrees from the inner pattern. Unlike the inner engines, each outer engine was gimbaled. That is, each could be swung through an arc. They were gimbaled as a means of steering the rocket, by letting the instrumentation of the rocket correct any deviations of its powered trajectory. The block I required engine gimabling as the only method of guiding and stabilizing the rocket through the lower atmosphere. The upper stages of the Block I rocket reflected the three-stage configuration of the Saturn I vehicle. During the SA-3 flight, the upper stage ejected 113,560 liters (30,000 gallons) of ballast water in the upper atmosphere for 'Project Highwater' physics experiment. The water was released at an altitude of 65 miles, where within only 5 seconds, it expanded into a massive ice cloud 4.6 miles in diameter. Release of this vast

  11. The preliminary study of autophagy induction of SA and MeSA by confocal

    NASA Astrophysics Data System (ADS)

    Yun, Lijuan; Chen, Wenli

    2010-02-01

    Autophagy appears to be a highly conserved process from unicellular to multicellular eukaryotes which contributes to the equilibrium of intracelluar environment. While it would be harmful to the cells when it is excessive by inducing programmed cell death (PCD). It is a protein degradation process in which cells recycle cytoplasmic contents when subjected to environmental stress conditions or during certain stages of development. Previous studies have demonstrated autophagy can be induced during abiotic or biotic stresses. salicylic acid (SA) and methyl salicytic (MeSA) are endogenous signal molecules. We found SA and MeSA can induce autophagy in Arabidopsis thaliana respectively. While autophagy was not induced by SA or MeSA in tobacco suspension cells under the same concentration and period. The differences in stuctures or physiological states may contribute to the results.

  12. SA3654 Component characterization. Final report

    SciTech Connect

    Meir, G.W.

    1996-06-01

    AlliedSignal Inc., Federal Manufacturing & Technologies (FM&T), was provided with production capability assurance program (PCAP) funding to develop, characterize, and qualify purchased product components for use on the PRESS-A program. The SA3654, N-Channel, Power MOSFET was identified as a component needing such activity to support PRESS-A. This report presents the characterization activities and results for the SA3654.

  13. Rb-Sr and Sm-Nd Isotopic Studies of Martian Depleted Shergottes SaU 094/005

    NASA Technical Reports Server (NTRS)

    Shih, C.-Y.; Nyquist, L. E.; Reese, Y.

    2007-01-01

    Sayh al Uhaymir (SaU) 094 and SaU 005 are olivine-phyric shergottites from the Oman desert and are considered as pairs. [e.g., 1]. They are very similar to the Libyan desert shergottite Dar al Gani (DaG) 476 in petrology, chemistry and ejection age [2-6]. This group of shergottites, also recognized as depleted shergottites [e.g. 7] has been strongly shocked and contains very low abundances of light rare earth elements (REE). In addition, terrestrial contaminants are commonly present in meteorites found in desert environments. Age-dating these samples is very challenging, but lower calcite contents in the SaU meteorites suggest that they have been subjected to less severe desert weathering than their DaG counterparts [3-4]. In this report, we present Rb-Sr and Sm-Nd isotopic results for SaU 094 and SaU 005, discuss the correlation of their ages with those of other similar shergottites, and discuss their petrogenesis.

  14. A Generalized Eigensolver based on Smoothed Aggregation (GES-SA) for Initializing Smoothed Aggregation Multigrid (SA)

    SciTech Connect

    Brezina, M; Manteuffel, T; McCormick, S; Ruge, J; Sanders, G; Vassilevski, P S

    2007-05-31

    Consider the linear system Ax = b, where A is a large, sparse, real, symmetric, and positive definite matrix and b is a known vector. Solving this system for unknown vector x using a smoothed aggregation multigrid (SA) algorithm requires a characterization of the algebraically smooth error, meaning error that is poorly attenuated by the algorithm's relaxation process. For relaxation processes that are typically used in practice, algebraically smooth error corresponds to the near-nullspace of A. Therefore, having a good approximation to a minimal eigenvector is useful to characterize the algebraically smooth error when forming a linear SA solver. This paper discusses the details of a generalized eigensolver based on smoothed aggregation (GES-SA) that is designed to produce an approximation to a minimal eigenvector of A. GES-SA might be very useful as a standalone eigensolver for applications that desire an approximate minimal eigenvector, but the primary aim here is for GES-SA to produce an initial algebraically smooth component that may be used to either create a black-box SA solver or initiate the adaptive SA ({alpha}SA) process.

  15. NormaCurve: A SuperCurve-Based Method That Simultaneously Quantifies and Normalizes Reverse Phase Protein Array Data

    PubMed Central

    Troncale, Sylvie; Barbet, Aurélie; Coulibaly, Lamine; Henry, Emilie; He, Beilei; Barillot, Emmanuel; Dubois, Thierry; Hupé, Philippe; de Koning, Leanne

    2012-01-01

    Motivation Reverse phase protein array (RPPA) is a powerful dot-blot technology that allows studying protein expression levels as well as post-translational modifications in a large number of samples simultaneously. Yet, correct interpretation of RPPA data has remained a major challenge for its broad-scale application and its translation into clinical research. Satisfying quantification tools are available to assess a relative protein expression level from a serial dilution curve. However, appropriate tools allowing the normalization of the data for external sources of variation are currently missing. Results Here we propose a new method, called NormaCurve, that allows simultaneous quantification and normalization of RPPA data. For this, we modified the quantification method SuperCurve in order to include normalization for (i) background fluorescence, (ii) variation in the total amount of spotted protein and (iii) spatial bias on the arrays. Using a spike-in design with a purified protein, we test the capacity of different models to properly estimate normalized relative expression levels. The best performing model, NormaCurve, takes into account a negative control array without primary antibody, an array stained with a total protein stain and spatial covariates. We show that this normalization is reproducible and we discuss the number of serial dilutions and the number of replicates that are required to obtain robust data. We thus provide a ready-to-use method for reliable and reproducible normalization of RPPA data, which should facilitate the interpretation and the development of this promising technology. Availability The raw data, the scripts and the NormaCurve package are available at the following web site: http://microarrays.curie.fr. PMID:22761696

  16. 76 FR 13063 - Airworthiness Directives; EUROCOPTER FRANCE Model SA330F, SA330G, and SA330J Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ... September 13, 2010 (75 FR 55492). That NPRM proposed to correct an unsafe condition for the specified... Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a significant economic impact, positive or... of the pedal unit on a SA 330 helicopter, the copilot set the position beyond the end limit...

  17. R4SA for Controlling Robots

    NASA Technical Reports Server (NTRS)

    Aghazarian, Hrand

    2009-01-01

    The R4SA GUI mentioned in the immediately preceding article is a userfriendly interface for controlling one or more robot(s). This GUI makes it possible to perform meaningful real-time field experiments and research in robotics at an unmatched level of fidelity, within minutes of setup. It provides such powerful graphing modes as that of a digitizing oscilloscope that displays up to 250 variables at rates between 1 and 200 Hz. This GUI can be configured as multiple intuitive interfaces for acquisition of data, command, and control to enable rapid testing of subsystems or an entire robot system while simultaneously performing analysis of data. The R4SA software establishes an intuitive component-based design environment that can be easily reconfigured for any robotic platform by creating or editing setup configuration files. The R4SA GUI enables event-driven and conditional sequencing similar to those of Mars Exploration Rover (MER) operations. It has been certified as part of the MER ground support equipment and, therefore, is allowed to be utilized in conjunction with MER flight hardware. The R4SA GUI could also be adapted to use in embedded computing systems, other than that of the MER, for commanding and real-time analysis of data.

  18. VIEW LOOKING EAST, SA WETSIDE (DISTILLATION BUILDING) ON THE RIGHT, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW LOOKING EAST, SA WETSIDE (DISTILLATION BUILDING) ON THE RIGHT, STD (SODA TOWER DRYERS?), SA DRYSIDE ON RIGHT. BEHIND STD BUILDING IS SHD BUILDING (SODA HORIZONTAL DRYERS?) THE ENTIRE DRYING COMPLEX WAS KNOWN AS THE DRYSIDE. - Solvay Process Company, SA Wetside Building, Between Willis & Milton Avenue, Solvay, Onondaga County, NY

  19. Whole-Genome Sequence of Leptospira interrogans Serovar Hardjo Subtype Hardjoprajitno Strain Norma, Isolated from Cattle in a Leptospirosis Outbreak in Brazil.

    PubMed

    Cosate, M R V; Soares, S C; Mendes, T A; Raittz, R T; Moreira, E C; Leite, R; Fernandes, G R; Haddad, J P A; Ortega, J Miguel

    2015-01-01

    Leptospirosis is caused by pathogenic bacteria of the genus Leptospira spp. This neglected re-emergent disease has global distribution and relevance in veterinary production. Here, we report the whole-genome sequence and annotation of Leptospira interrogans serovar Hardjo subtype Hardjoprajitno strain Norma, isolated from cattle in a livestock leptospirosis outbreak in Brazil. PMID:26543126

  20. Whole-Genome Sequence of Leptospira interrogans Serovar Hardjo Subtype Hardjoprajitno Strain Norma, Isolated from Cattle in a Leptospirosis Outbreak in Brazil

    PubMed Central

    Soares, S. C.; Mendes, T. A.; Raittz, R. T.; Moreira, E. C.; Leite, R.; Fernandes, G. R.; Haddad, J. P. A.; Ortega, J. Miguel

    2015-01-01

    Leptospirosis is caused by pathogenic bacteria of the genus Leptospira spp. This neglected re-emergent disease has global distribution and relevance in veterinary production. Here, we report the whole-genome sequence and annotation of Leptospira interrogans serovar Hardjo subtype Hardjoprajitno strain Norma, isolated from cattle in a livestock leptospirosis outbreak in Brazil. PMID:26543126

  1. Development and evaluation of serodiagnostic assays with recombinant BgSA1 of Babesia gibsoni.

    PubMed

    Mandal, Mrityunjay; Banerjee, Partha S; Kumar, Saroj; Garg, Rajat; Ram, Hira; Kundu, Krishnendu; Raina, Opinder K

    2014-10-15

    Indirect ELISA, dot-ELISA and double antibody sandwich ELISA (DAS-ELISA) using truncated recombinant BgSA1 (rBgSA1) were developed for detecting Babesia gibsoni infection in naturally infected dogs. Truncated BgSA1 gene of 858 bp, encoding 32 kDa protein was cloned in pET-32a(+) expression vector, expressed in Escherichia coli and the recombinant protein was purified under native conditions. To evaluate the ability of the truncated rBgSA1as serodiagnostic reagent for B. gibsoni infection, a panel of sera/plasma samples from dogs infected with B. gibsoni (n = 13), uninfected sera (n = 13) and sera from dogs infected with other haemoparasites namely, Babesia canis vogeli (n = 3), Ehrlichia canis (n = 3), Hepatozoon canis (n = 1) and Dirofilaria immitis (n = 1) were used. Besides these, 75 samples collected from dogs suspected for babesiosis were used to evaluate the performance of rBgSA1 based serological assays in comparison to nested PCR. Based on the results, the diagnostic sensitivity of indirect ELISA, dot-ELISA and DAS-ELISA were 97.3%, 91.9% and 100%, respectively, when nested PCR was taken as a reference test, while their specificities were 81.6%, 84.2% and 97.4%, respectively. Further, DAS-ELISA had a quantitation limit of 0.03 μg/ml of the rBgSA1. High kappa values of indirect ELISA, dot-ELISA and DAS-ELISA were recorded, indicating that these assays had substantial to almost perfect agreement at 95% confidence level. There was no cross-reactivity with sera from dogs infected with B. canis vogeli, E. canis, H. canis and D. immitis. The results suggest that the indirect ELISA, dot-ELISA and DAS-ELISA with rBgSA1 may be used in large scale epidemiological surveys and clinical diagnosis of B. gibsoni infection in dogs. DAS-ELISA has advantages over indirect or dot-ELISA in the detection of current infection as well as monitoring the parasite burden. PMID:25241636

  2. Atmospheric environment for ASTP (SA-210) launch

    NASA Technical Reports Server (NTRS)

    Johnson, D. L.

    1976-01-01

    A summary is presented of selected atmospheric conditions observed near ASTP/SA-210 launch time on July 15, 1975, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), density, index of refraction, and wind/wind shear aloft are included. A final meteorological data tape for the ASTP launch, consisting of wind and thermodynamic parameters versus altitude, has been constructed.

  3. Saturn I (SA-1) on Launch Pad

    NASA Technical Reports Server (NTRS)

    1961-01-01

    On October 27, 1961, the Marshall Space Flight Center and the Nation marked a high point in the 3-year-old Saturn development program when the first Saturn vehicle, SA-1, flew a flawless 215-mile ballistic trajectory from Cape Canaveral, Florida. SA-1 is pictured here on the launch pad ready for lift off. Developed at the Marshall Space Flight Center (MSFC) under the direction of Dr. Wernher von Braun, SA-1 incorporated a Saturn I, Block I engine. The typical height of a Block I vehicle was approximately 163 feet and had only one live stage. It consisted of eight tanks, each 70 inches in diameter, clustered around a central tank, 105 inches in diameter. Four of the external tanks were fuel tanks for the RP-1 (kerosene) fuel. The other four, spaced alternately with the fuel tanks, were liquid oxygen tanks, as was the large center tank. All fuel tanks and liquid oxygen tanks drained at the same rates respectively. The thrust for the stage came from eight H-1 engines, each producing a thrust of 165,000 pounds, for a total thrust of over 1,300,000 pounds. The engines were arranged in a double pattern. Four engines, located inboard, were fixed in a square pattern around the stage axis and canted outward slightly, while the remaining four engines were located outboard in a larger square pattern offset 40 degrees from the inner pattern. Unlike the inner engines, each outer engine was gimbaled. That is, each could be swung through an arc. They were gimbaled as a means of steering the rocket, by letting the instrumentation of the rocket correct any deviations of its powered trajectory. The block I required engine gimabling as the only method of guiding and stabilizing the rocket through the lower atmosphere. The upper stages of the Block I rocket reflected the three-stage configuration of the Saturn I vehicle.

  4. DoSA: Database of Structural Alignments.

    PubMed

    Mahajan, Swapnil; Agarwal, Garima; Iftekhar, Mohammed; Offmann, Bernard; de Brevern, Alexandre G; Srinivasan, Narayanaswamy

    2013-01-01

    Protein structure alignment is a crucial step in protein structure-function analysis. Despite the advances in protein structure alignment algorithms, some of the local conformationally similar regions are mislabeled as structurally variable regions (SVRs). These regions are not well superimposed because of differences in their spatial orientations. The Database of Structural Alignments (DoSA) addresses this gap in identification of local structural similarities obscured in global protein structural alignments by realigning SVRs using an algorithm based on protein blocks. A set of protein blocks is a structural alphabet that abstracts protein structures into 16 unique local structural motifs. DoSA provides unique information about 159,780 conformationally similar and 56,140 conformationally dissimilar SVRs in 74 705 pairwise structural alignments of homologous proteins. The information provided on conformationally similar and dissimilar SVRs can be helpful to model loop regions. It is also conceivable that conformationally similar SVRs with conserved residues could potentially contribute toward functional integrity of homologues, and hence identifying such SVRs could be helpful in understanding the structural basis of protein function. Database URL: http://bo-protscience.fr/dosa/ PMID:23846594

  5. DoSA: Database of Structural Alignments

    PubMed Central

    Mahajan, Swapnil; Agarwal, Garima; Iftekhar, Mohammed; Offmann, Bernard; de Brevern, Alexandre G.; Srinivasan, Narayanaswamy

    2013-01-01

    Protein structure alignment is a crucial step in protein structure–function analysis. Despite the advances in protein structure alignment algorithms, some of the local conformationally similar regions are mislabeled as structurally variable regions (SVRs). These regions are not well superimposed because of differences in their spatial orientations. The Database of Structural Alignments (DoSA) addresses this gap in identification of local structural similarities obscured in global protein structural alignments by realigning SVRs using an algorithm based on protein blocks. A set of protein blocks is a structural alphabet that abstracts protein structures into 16 unique local structural motifs. DoSA provides unique information about 159 780 conformationally similar and 56 140 conformationally dissimilar SVRs in 74 705 pairwise structural alignments of homologous proteins. The information provided on conformationally similar and dissimilar SVRs can be helpful to model loop regions. It is also conceivable that conformationally similar SVRs with conserved residues could potentially contribute toward functional integrity of homologues, and hence identifying such SVRs could be helpful in understanding the structural basis of protein function. Database URL: http://bo-protscience.fr/dosa/ PMID:23846594

  6. Ammonia Offgassing from SA9T

    NASA Technical Reports Server (NTRS)

    Monje, Oscar; Nolek, Sara D.; Wheeler, Raymond M.

    2011-01-01

    NH3 is a degradation product of SA9T, a solid-amine sorbent developed by Hamilton Sundstrand, that is continually emitted into the gas stream being conditioned by this sorbent. NH3 offgassing rates were measured using FTIR spectroscopy using a packed bed at similar contact times as offgassing tests conducted at Hamilton Sundstrand and at the Ames Research Center. The bed was challenged with moist air at several flow rates and humidities and NH3 concentration of the effluent was measured for several hours. The NH3 offgassing rates in open-loop testing were calculated from the steady state outlet NH3 concentration and flow rate. NH3 offgassing rates from SA9T were found to be influenced by the contact time with the adsorbent (flow rate) and by the humidity of the inlet gas stream, which are consistent with previous studies. Closed-loop vacuum-swing adsorption cycling rates verified that NH3 offgassing continues when a constant source of water vapor is present.

  7. Investigating CXOU J163802.6–471358: A new pulsar wind nebula in the norma region?

    SciTech Connect

    Jakobsen, Simone J.; Watson, Darach; Tomsick, John A.; Gotthelf, Eric V.; Kaspi, Victoria M.

    2014-06-01

    We present the first analysis of the extended source CXOU J163802.6–471358, which was discovered serendipitously during the Chandra X-ray survey of the Norma region of the Galactic spiral arms. The X-ray source exhibits a cometary appearance with a point source and an extended tail region. The complete source spectrum is fitted well with an absorbed power law model and jointly fitting the Chandra spectrum of the full source with one obtained from an archived XMM-Newton observation results in best fit parameters N {sub H} =1.5{sub −0.5}{sup +0.7}×10{sup 23} cm{sup −2} and Γ=1.1{sub −0.6}{sup +0.7} (90% confidence uncertainties). The unabsorbed luminosity of the full source is then L{sub X}∼4.8×10{sup 33}d{sub 10}{sup 2} erg s{sup –1} with d {sub 10} = d/10 kpc, where a distance of 10 kpc is a lower bound inferred from the large column density. The radio counterpart found for the source using data from the Molonglo Galactic Plane Survey epoch-2 shows an elongated tail offset from the X-ray emission. No infrared counterpart was found. The results are consistent with the source being a previously unknown pulsar driving a bow shock through the ambient medium.

  8. EXTERIOR VIEW, THE SA WETSIDE BUILDING (ALSO KNOWN AS DISTILLATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    EXTERIOR VIEW, THE SA WETSIDE BUILDING (ALSO KNOWN AS DISTILLATION BUILDING OR SOLVAY TOWERS BUILDING) THE HEART OF THE SOLVAY PROCESS. VIEW LOOKING EAST. THIS BUILDING WAS BUILT IN 1954 TO REPLACE A SIMILAR STRUCTURE BUILT CIRCA 1907. - Solvay Process Company, SA Wetside Building, Between Willis & Milton Avenue, Solvay, Onondaga County, NY

  9. SEU characterization and design dependence of the SA3300 microprocessor

    SciTech Connect

    Sexton, F.W.; Treece, R.K.; Axness, C.L.; Hughes, K.H.; Hash, G.L.; Hass, K.J.

    1990-01-01

    The SA3300 16-bit microprocessor is a key component for interplanetary space probes to be launched in the 1990's. A full SEU characterization of the D-latches of the SA3300 is given, including pattern sensitivity and temperature. 9 refs., 5 figs.

  10. 77 FR 65144 - Airworthiness Directives; Embraer S.A. Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-25

    ... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Embraer S.A...). SUMMARY: We propose to adopt a new airworthiness directive (AD) for certain Embraer S.A. Model ERJ 170...

  11. Inhibition of star formation in Sa galaxies

    SciTech Connect

    Pompea, S.M.; Rieke, G.H. )

    1989-07-01

    Only 4 percent of Sas in the Revised Shapley-Ames Catalog with B(T) less than 12 have an infrared luminosity greater than 10 to the 10th solar. This proportion is about one-sixth of the corresponding one for Sbs and Scs. Although the infrared luminosities of most Sa galaxies are dominated by disk emission, the same trend appears in the incidence of nuclear starbursts. IRAS measurements indicate that no more than three Sas out of the entire RSA sample of 166 galaxies have nuclear starbursts that cannot be associated with interactions or active nuclei. Plots of H I fluxes do not strongly correlate with infrared fluxes. Similarly, for at least the infrared selected Sas, the trend of IR flux with CO flux is similar to that of later type spiral galaxies. This would imply that molecular cloud formation is inhibited in Sas, leading to the lack of infrared activity. 38 refs.

  12. STS-48 ESC closeup of UARS solar array (SA) and SA mechanism, pre-deploy

    NASA Technical Reports Server (NTRS)

    1991-01-01

    An extremely closeup view shows the Upper Atmosphere Research Satellite (UARS) solar array (SA) and SA mechanism prior to deploy of the satellite. UARS, grappled by the remote manipulator system (RMS) end effector (out of frame), is undergoing STS-48 pre-deployment checkout above the payload bay (PLB) of the earth-orbiting Discovery, Orbiter Vehicle (OV) 103. OV-103's vertical stabilizer can be seen in between the UARS hardware. This view demonstrates the capabilities of the Electronic Still Camera (ESC) to provide high resolution views of hardware for review by ground controllers. This ESC image was documented as part of Development Test Objective (DTO) 648, Electronic Still Photography. The digital image was stored on a removable hard disk or small optical disk, and could be converted to a format suitable for downlink transmission.

  13. Cloning, Characterization and Expression Pattern Analysis of a Cytosolic Copper/Zinc Superoxide Dismutase (SaCSD1) in a Highly Salt Tolerant Mangrove (Sonneratia alba)

    PubMed Central

    Yang, Enze; Yi, Shanze; Bai, Fang; Niu, Dewei; Zhong, Junjie; Wu, Qiuhong; Chen, Shufang; Zhou, Renchao; Wang, Feng

    2015-01-01

    Mangroves are critical marine resources for their remarkable ability to tolerate seawater. Antioxidant enzymes play an especially significant role in eliminating reactive oxygen species and conferring abiotic stress tolerance. In this study, a cytosolic copper/zinc superoxide dismutase (SaCSD1) cDNA of Sonneratia alba, a mangrove species with high salt tolerance, was successfully cloned and then expressed in Escherichia coli Rosetta-gami (designated as SaCSD1). SaCSD1 comprised a complete open reading frame (ORF) of 459 bp which encoded a protein of 152 amino acids. Its mature protein is predicted to be 15.32 kDa and the deduced isoelectric point is 5.78. SaCSD1 has high sequence similarity (85%–90%) with the superoxide dismutase (CSD) of some other plant species. SaCSD1 was expressed with 30.6% yield regarding total protein content after being introduced into the pET-15b (Sma I) vector for expression in Rosetta-gami and being induced with IPTG. After affinity chromatography on Ni-NTA, recombinant SaCSD1 was obtained with 3.2-fold purification and a specific activity of 2200 U/mg. SaCSD1 showed good activity as well as stability in the ranges of pH between 3 and 7 and temperature between 25 and 55 °C. The activity of recombinant SaCSD1 was stable in 0.25 M NaCl, Dimethyl Sulphoxide (DMSO), glycerol, and chloroform, and was reduced to a great extent in β-mercaptoethanol, sodium dodecyl sulfate (SDS), H2O2, and phenol. Moreover, the SaCSD1 protein was very susceptive to pepsin digestion. Real-time Quantitative Polymerase Chain Reaction (PCR) assay demonstrated that SaCSD1 was expressed in leaf, stem, flower, and fruit organs, with the highest expression in fruits. Under 0.25 M and 0.5 M salt stress, the expression of SaCSD1 was down-regulated in roots, but up-regulated in leaves. PMID:26703583

  14. Cloning, Characterization and Expression Pattern Analysis of a Cytosolic Copper/Zinc Superoxide Dismutase (SaCSD1) in a Highly Salt Tolerant Mangrove (Sonneratia alba).

    PubMed

    Yang, Enze; Yi, Shanze; Bai, Fang; Niu, Dewei; Zhong, Junjie; Wu, Qiuhong; Chen, Shufang; Zhou, Renchao; Wang, Feng

    2016-01-01

    Mangroves are critical marine resources for their remarkable ability to tolerate seawater. Antioxidant enzymes play an especially significant role in eliminating reactive oxygen species and conferring abiotic stress tolerance. In this study, a cytosolic copper/zinc superoxide dismutase (SaCSD1) cDNA of Sonneratia alba, a mangrove species with high salt tolerance, was successfully cloned and then expressed in Escherichia coli Rosetta-gami (designated as SaCSD1). SaCSD1 comprised a complete open reading frame (ORF) of 459 bp which encoded a protein of 152 amino acids. Its mature protein is predicted to be 15.32 kDa and the deduced isoelectric point is 5.78. SaCSD1 has high sequence similarity (85%-90%) with the superoxide dismutase (CSD) of some other plant species. SaCSD1 was expressed with 30.6% yield regarding total protein content after being introduced into the pET-15b (Sma I) vector for expression in Rosetta-gami and being induced with IPTG. After affinity chromatography on Ni-NTA, recombinant SaCSD1 was obtained with 3.2-fold purification and a specific activity of 2200 U/mg. SaCSD1 showed good activity as well as stability in the ranges of pH between 3 and 7 and temperature between 25 and 55 °C. The activity of recombinant SaCSD1 was stable in 0.25 M NaCl, Dimethyl Sulphoxide (DMSO), glycerol, and chloroform, and was reduced to a great extent in β-mercaptoethanol, sodium dodecyl sulfate (SDS), H₂O2, and phenol. Moreover, the SaCSD1 protein was very susceptive to pepsin digestion. Real-time Quantitative Polymerase Chain Reaction (PCR) assay demonstrated that SaCSD1 was expressed in leaf, stem, flower, and fruit organs, with the highest expression in fruits. Under 0.25 M and 0.5 M salt stress, the expression of SaCSD1 was down-regulated in roots, but up-regulated in leaves. PMID:26703583

  15. Cyclic-load crack growth in ASME SA-105 grade II steel in high-pressure hydrogen at ambient temperature

    NASA Technical Reports Server (NTRS)

    Walter, R. J.; Chandler, W. T.

    1976-01-01

    ASME SA-105 Grade II steel, which is used in high-pressure hydrogen compressor systems, is similar to steels used or considered for use in high-pressure hydrogen storage vessels and pipelines. This paper summarizes the results of a program conducted to provide cyclic-load crack growth rate (da/dN) data for a fracture mechanics analysis of a 15,000 psi hydrogen compressor facility which contains pulse quieter and after-cooler separator vessels constructed of the ASME SA-105 Grade II steel. Included in the program were tests performed to assist in establishing operating procedures that could minimize the effect of hydrogen on crack growth rates during operation.

  16. PKC phosphorylates residues in the N-terminal of the DA transporter to regulate amphetamine-induced DA efflux.

    PubMed

    Wang, Qiang; Bubula, Nancy; Brown, Jason; Wang, Yunliang; Kondev, Veronika; Vezina, Paul

    2016-05-27

    The DA transporter (DAT), a phosphoprotein, controls extracellular dopamine (DA) levels in the central nervous system through transport or reverse transport (efflux). Multiple lines of evidence support the claim that PKC significantly contributes to amphetamine-induced DA efflux. Other signaling pathways, involving CaMKII and ERK, have also been shown to regulate DAT mediated efflux. Here we assessed the contribution of putative PKC residues (S4, S7, S13) in the N-terminal of the DAT to amphetamine-induced DA efflux by transfecting DATs containing different serine to alanine (S-A) point mutations into DA pre-loaded HEK-293 cells and incubating these cells in amphetamine (2μM). The effects of a S-A mutation at the non-PKC residue S12 and a threonine to alanine (T-A) mutation at the ERK T53 residue were also assessed for comparison. WT-DATs were used as controls. In an initial experiment, we confirmed that inhibiting PKC with Go6976 (130nM) significantly reduced amphetamine-induced DA efflux. In subsequent experiments, cells transfected with the S4A, S12A, S13A, T53A and S4,7,13A mutants showed a reduction in amphetamine-induced DA efflux similar to that observed with Go6976. Interestingly, cells transfected with the S7A mutant, identified by some as a PKC-PKA residue, showed unperturbed WT-DAT levels of amphetamine-induced DA efflux. These results indicate that phosphorylation by PKC of select residues in the DAT N-terminal can regulate amphetamine-induced efflux. PKC can act either independently or in concert with other kinases such as ERK to produce this effect. PMID:27113203

  17. Etnografía acelerada para transformar normas sociales sobre género y sexualidad en hombres puertorriqueños heterosexuales1,2

    PubMed Central

    Ortiz-Torres, Blanca; Rivera-Ortiz, Rafael J.; Mendoza, Sigrid

    2014-01-01

    Resumen La construcción de roles de género dominantes contribuyen al riesgo de contraer VIH, y por tal razón se ha urgido a que se integren las normas sociales relativas al género en las intervenciones preventivas del VIH. Este estudio pretende adaptar y desarrollar una intervención que facilite la transformación de normas sociales del género y de prácticas sexuales en hombres puertorriqueños. La intervención propone transformar normas sociales relacionadas al género y sexualidad en barras comunitarias utilizando el modelo de líderes de opinión. Luego de ser elegidos/as, los/as líderes de opinión diseminan mensajes integrando la importancia de relaciones equitativas entre parejas para la prevención del VIH. La primera fase de esta intervención es discutida en este artículo, la cual incluye un proceso de etnografía acelerada para identificar los escenarios comunitarios en los que podemos desarrollar esta intervención y permitirnos entender la cultura de las barras comunitarias. A partir de las observaciones etnográficas, pudimos: desarrollar un protocolo de seguridad para realizar las observaciones, desarrollar un perfil de la cultura de las barras, elegir las barras a participar en las dos condiciones del estudio y adaptar los instrumentos de la intervención para que respondieran a la particularidad de los/as participantes. PMID:25530828

  18. Realtime mitigation of GPS SA errors using Loran-C

    NASA Technical Reports Server (NTRS)

    Braasch, Soo Y.

    1994-01-01

    The hybrid use of Loran-C with the Global Positioning System (GPS) was shown capable of providing a sole-means of enroute air radionavigation. By allowing pilots to fly direct to their destinations, use of this system is resulting in significant time savings and therefore fuel savings as well. However, a major error source limiting the accuracy of GPS is the intentional degradation of the GPS signal known as Selective Availability (SA). SA-induced position errors are highly correlated and far exceed all other error sources (horizontal position error: 100 meters, 95 percent). Realtime mitigation of SA errors from the position solution is highly desirable. How that can be achieved is discussed. The stability of Loran-C signals is exploited to reduce SA errors. The theory behind this technique is discussed and results using bench and flight data are given.

  19. Comparative Analysis of Simulated Annealing (SA) and Simplified Generalized SA (SGSA) for Estimation Optimal of Parametric Functional in CATIVIC

    SciTech Connect

    Freitez, Juan A.; Sanchez, Morella; Ruette, Fernando

    2009-08-13

    Application of simulated annealing (SA) and simplified GSA (SGSA) techniques for parameter optimization of parametric quantum chemistry method (CATIVIC) was performed. A set of organic molecules were selected for test these techniques. Comparison of the algorithms was carried out for error function minimization with respect to experimental values. Results show that SGSA is more efficient than SA with respect to computer time. Accuracy is similar in both methods; however, there are important differences in the final set of parameters.

  20. The JT-60SA Cryoplant Current Design Status

    NASA Astrophysics Data System (ADS)

    Henry, D.; Michel, F.; Roussel, P.; Reynaud, P.; Journeaux, J. Y.; Maréchal, J. L.; Balaguer, D.; Roux, C.; Matsukawa, M.; Yoshida, K.

    2008-03-01

    In the framework of the ITER Broader Approach, CEA is carrying out the procurement of the Cryogenic System to the JA-EU Satellite Tokamak JT-60SA, which should be operated in Japan at JAEA, Naka in 2014. According to the Conceptual Design Report, JT-60SA is to operate for periods of at least 6 months per year, with major shutdown periods in between for maintenance and further installation upgrades. For this operation scenario, the cryoplant and the cryodistribution have to cope with different heat loads which depend on the different JT-60SA operating states. The cryoplant consists of one 4.5 K refrigerator and one 80 K helium loop, each pre-cooled by LN2. These cryogenic subsystems have to operate simultaneously in order to remove the heat loads from the superconducting magnets, 80 K shields and the divertor cryopumps. The first part of this study is based on the Process Flow Diagram (PFD) and presents the current design status of the JT-60SA cryogenic system. The second part is dedicated to the analysis of the cryoplant normal operation modes including the regeneration mode of the divertor cryopumps. Thanks to this analysis, the architecture of the present PFD is proposed in order to match the technical specifications of the cryoplant with the JT-60SA operation requirements.

  1. Uhuru observations of 4U 1608-52 - The 'steady' X-ray source associated with the X-ray burst source in Norma

    NASA Technical Reports Server (NTRS)

    Tananbaum, H.; Chaisson, L. J.; Forman, W.; Jones, C.; Matilsky, T. A.

    1976-01-01

    Data are presented for the X-ray source 4U 1608-52, summarizing its light curve, location, and spectral parameters. Evidence is presented showing that this source is the 'steady' X-ray counterpart of the X-ray burst source in Norma. The spectrum of the 'steady' source is compared with the spectrum observed during two bursts, and it is noted that there is substantially more low-energy absorption during the bursts. The 'steady' source spectral data are used to examine the optical data, and it is concluded that if the X-ray spectrum is thermal, then a globular-cluster counterpart probably would have been detected (whereas none has been). Further X-ray and optical observations are suggested for this source, since an optical identification may be central in determining whether all X-ray bursts have a common origin and if this origin requires a globular-cluster environment.

  2. [Revista de Saúde Pública: 30 years of evolution].

    PubMed

    da Silva, L J

    1996-12-01

    Analysis of the main characteristics of the "Revista de Saúde Pública" during the 30 years of its existence. A parallel is traced with the evolution of brazilian public health. The dynamic aspect of the "Revista" is remembered as one of its main virtues. Its future in the constitution of the Mercosul regional block is discussed. PMID:9302818

  3. 78 FR 52416 - Airworthiness Directives; Embraer S.A. Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-23

    ... February 22, 2013 (78 FR ] 12256). That NPRM proposed to correct an unsafe condition for the specified... Accordance With Previous Service Information Embraer S.A. requested that we revise the NPRM (78 FR 12256... changes: Are consistent with the intent that was proposed in the NPRM (78 FR 12256, February 22, 2013)...

  4. Multifunctional SA-PProDOT Binder for Lithium Ion Batteries.

    PubMed

    Ling, Min; Qiu, Jingxia; Li, Sheng; Yan, Cheng; Kiefel, Milton J; Liu, Gao; Zhang, Shanqing

    2015-07-01

    An environmentally benign, highly conductive, and mechanically strong binder system can overcome the dilemma of low conductivity and insufficient mechanical stability of the electrodes to achieve high performance lithium ion batteries (LIBs) at a low cost and in a sustainable way. In this work, the naturally occurring binder sodium alginate (SA) is functionalized with 3,4-propylenedioxythiophene-2,5-dicarboxylic acid (ProDOT) via a one-step esterification reaction in a cyclohexane/dodecyl benzenesulfonic acid (DBSA)/water microemulsion system, resulting in a multifunctional polymer binder, that is, SA-PProDOT. With the synergetic effects of the functional groups (e.g., carboxyl, hydroxyl, and ester groups), the resultant SA-PProDOT polymer not only maintains the outstanding binding capabilities of sodium alginate but also enhances the mechanical integrity and lithium ion diffusion coefficient in the LiFePO4 (LFP) electrode during the operation of the batteries. Because of the conjugated network of the PProDOT and the lithium doping under the battery environment, the SA-PProDOT becomes conductive and matches the conductivity needed for LiFePO4 LIBs. Without the need of conductive additives such as carbon black, the resultant batteries have achieved the theoretical specific capacity of LiFePO4 cathode (ca. 170 mAh/g) at C/10 and ca. 120 mAh/g at 1C for more than 400 cycles. PMID:26061529

  5. SA- AND NO- MEDIATED SIGNALLING IN PLANT DISEASE RESISTANCE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Salicylic acid and nitric oxide mediated signalling are two key regulators of plant disease resistance mechanisms. Using multiple Arabidopsis mutants that are positive or negative regulators of the SA response we are dissecting the signal transduction chain. To elucidate the components associated w...

  6. 77 FR 33334 - Airworthiness Directives; Embraer S.A. Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-06

    ... (75 FR 30277, June 1, 2010). (c) Applicability (1) This AD applies to Embraer S.A. Model ERJ 190-100...-14, Amendment 39-16319 (75 FR 30277, June 1, 2010). That AD required actions intended to address an unsafe condition on the products listed above. Since we issued AD 2010-11-14, Amendment 39-16319 (75...

  7. Rhein 8-O-β-D-Glucopyranoside Elicited the Purgative Action of Daiokanzoto (Da-Huang-Gan-Cao-Tang), Despite Dysbiosis by Ampicillin.

    PubMed

    Takayama, Kento; Tabuchi, Norihiko; Fukunaga, Masahito; Okamura, Nobuyuki

    2016-01-01

    Sennoside A (SA), the main purgative constituent of Daiokanzoto (da-huang-gan-cao-tang; DKT), is generally regarded as a prodrug that is transformed into an active metabolite by β-glucosidase derived from Bifidobacterium spp. It has been suggested that antibiotics would promote dysbiosis, and thereby inhibit the purgative activity of DKT. In this study, ampicillin was administered to mice for 8 d, and the changes in the SA metabolism of SA alone and of DKT were investigated. The results showed that the SA metabolism of SA singly continued to be inhibited by ampicillin, but that of DKT was activated from day 3 under the same conditions. In order to investigate the mechanism of SA metabolism activated by DKT in the mice administered ampicillin, changes in the SA metabolism were observed in the presence of rhein 8-O-β-D-glucopyranoside (RG) in rhubarb and liquiritin in glycyrrhiza, both of which accelerated the SA metabolism. In fact, RG achieved an activation of SA metabolism similar to that by DKT. The purgative action of DKT, which was continued treatment of the ampicillin, was significantly greater than that by SA alone, and it was shown that RG was involved in this effect. We also analyzed changes in the intestinal microbiota before and after administration of ampicillin. No Bifidobacteria were detected throughout the treatment, but the population of Bacteroides was significantly increased after 3 d under the same conditions. Taken together, these results strongly suggested that the RG in DKT changed the function of Bacteroides and thereby allowed DKT to metabolize SA. PMID:26934929

  8. Zitkala-Sa and the Problem of Regionalism: Nations, Narratives, and Critical Traditions

    ERIC Educational Resources Information Center

    Totten, Gary

    2005-01-01

    Although Yankton Sioux writer Zitkala-Sa (Gertrude Bonnin, 1876-1938) was, as P. Jane Hafen notes, "virtually unknown for many decades," much critical work has appeared since Dexter Fisher's 1979 article,"Zitkala-Sa: Evolution of a Writer." Some critics desiring to bring Zitkala-Sa into the conversation about turn-of-the-century American women…

  9. [Synergistion mechanism of exogenous Ca2+ to SA-induced resistance to Botrytis cinerea in tomato].

    PubMed

    Li, Lin-lin; Li, Tian-lai; Jiang, Guo-bin; Jin, Hua; Zou, Ji-xiang

    2015-11-01

    In this study, we investigated the effect of exogenous calcium and salicylic acid (SA) on Botrytis cinerea resistance in tomato seedlings. We treated a tomato strain susceptible to Botrytis cinerea with foliar spraying of water, SA, SA+CaCl2 and SA+EGTA (Ca2+ chelating agent) for one to five days. During the treatment, leaves were collected to analyze the reactive oxygen species (ROS) content, phenylalanine ammonia lyase (PAL) activity, chintase and β-1,3-glucanase levels, and the expression of pathogenesis related protein 1, 2, 3 (PR1, PR2, PR3). Three days after infection, the disease index was 74.8 in control plants, and 46.9, 38.5 and 70.3 in SA, SA+Ca and SA+ EGTA treated plants, respectively. SA treatment significantly increased ROS leaf accumulation, and activities of PAL, chintase and β-1,3-glucanase. These values were further enhanced in SA+Ca treated plants, but decreased in SA+EGTA treated plants. Application of SA significantly increased the expression levels of PR1, PR2a and PR3b, which were further elevated by the combination treatment with Ca2+. These effects were counteracted by the combination treatment of SA and EGTA. The transcription levels of PR2b and PR3a were up-regulated by 1-2 folds, and PR1, 2a and 3b by 2-5 folds in SA- and SA+Ca-treated plants relative to control. These data suggested that application of Ca2+ could synergistically increase SA-induced resistance to B. cinerea. The resistance was associated with ROS accumulation, therefore the increase in resistance might be through ROS ability to increase the activity of defense-related enzymes and expression levels of PR1, PR2a and PR3b. PMID:26915208

  10. Performance of JT-60SA divertor Thomson scattering diagnostics

    SciTech Connect

    Kajita, Shin; Hatae, Takaki; Tojo, Hiroshi; Hamano, Takashi; Shimizu, Katsuhiro; Kawashima, Hisato; Enokuchi, Akito

    2015-08-15

    For the satellite tokamak JT-60 Super Advanced (JT-60SA), a divertor Thomson scattering measurement system is planning to be installed. In this study, we improved the design of the collection optics based on the previous one, in which it was found that the solid angle of the collection optics became very small, mainly because of poor accessibility to the measurement region. By improvement, the solid angle was increased by up to approximately five times. To accurately assess the measurement performance, background noise was assessed using the plasma parameters in two typical discharges in JT-60SA calculated from the SONIC code. Moreover, the influence of the reflection of bremsstrahlung radiation by the wall is simulated by using a ray tracing simulation. The errors in the temperature and the density are assessed based on the simulation results for three typical field of views.

  11. Performance verification tests of JT-60SA CS model coil

    NASA Astrophysics Data System (ADS)

    Obana, Tetsuhiro; Murakami, Haruyuki; Takahata, Kazuya; Hamaguchi, Shinji; Chikaraishi, Hirotaka; Mito, Toshiyuki; Imagawa, Shinsaku; Kizu, Kaname; Natsume, Kyohei; Yoshida, Kiyoshi

    2015-11-01

    As a final check of the coil manufacturing method of the JT-60 Super Advanced (JT-60SA) central solenoid (CS), we verified the performance of a CS model coil. The model coil comprised a quad-pancake wound with a Nb3Sn cable-in-conduit conductor. Measurements of the critical current, joint resistance, pressure drop, and magnetic field were conducted in the verification tests. In the critical-current measurement, the critical current of the model coil coincided with the estimation derived from a strain of -0.62% for the Nb3Sn strands. As a result, critical-current degradation caused by the coil manufacturing process was not observed. The results of the performance verification tests indicate that the model coil met the design requirements. Consequently, the manufacturing process of the JT-60SA CS was established.

  12. Performance of JT-60SA divertor Thomson scattering diagnostics.

    PubMed

    Kajita, Shin; Hatae, Takaki; Tojo, Hiroshi; Enokuchi, Akito; Hamano, Takashi; Shimizu, Katsuhiro; Kawashima, Hisato

    2015-08-01

    For the satellite tokamak JT-60 Super Advanced (JT-60SA), a divertor Thomson scattering measurement system is planning to be installed. In this study, we improved the design of the collection optics based on the previous one, in which it was found that the solid angle of the collection optics became very small, mainly because of poor accessibility to the measurement region. By improvement, the solid angle was increased by up to approximately five times. To accurately assess the measurement performance, background noise was assessed using the plasma parameters in two typical discharges in JT-60SA calculated from the SONIC code. Moreover, the influence of the reflection of bremsstrahlung radiation by the wall is simulated by using a ray tracing simulation. The errors in the temperature and the density are assessed based on the simulation results for three typical field of views. PMID:26329196

  13. Using SA508/533 for the HTGR Vessel Material

    SciTech Connect

    Larry Demick

    2012-06-01

    This paper examines the influence of High Temperature Gas-cooled Reactor (HTGR) module power rating and normal operating temperatures on the use of SA508/533 material for the HTGR vessel system with emphasis on the calculated times at elevated temperatures approaching or exceeding ASME Code Service Limits (Levels B&C) to which the reactor pressure vessel could be exposed during postulated pressurized and depressurized conduction cooldown events over its design lifetime.

  14. Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97

    PubMed Central

    Chang, Yoonjee; Shin, Hakdong; Lee, Ju-Hoon; Park, Chul Jong; Paik, Soon-Young; Ryu, Sangryeol

    2015-01-01

    A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97. Genome analysis revealed that SA97 contains 40,592 bp of DNA encoding 54 predicted open reading frames (ORFs), and none of these genes were related to virulence or drug resistance. Although a few genes associated with lysogen formation were detected in the phage SA97 genome, the phage SA97 produced neither lysogen nor transductant in S. aureus. These results suggest that the phage SA97 may be a promising candidate for controlling S. aureus. PMID:26437428

  15. Solving Fuzzy Optimization Problem Using Hybrid Ls-Sa Method

    NASA Astrophysics Data System (ADS)

    Vasant, Pandian

    2011-06-01

    Fuzzy optimization problem has been one of the most and prominent topics inside the broad area of computational intelligent. It's especially relevant in the filed of fuzzy non-linear programming. It's application as well as practical realization can been seen in all the real world problems. In this paper a large scale non-linear fuzzy programming problem has been solved by hybrid optimization techniques of Line Search (LS), Simulated Annealing (SA) and Pattern Search (PS). As industrial production planning problem with cubic objective function, 8 decision variables and 29 constraints has been solved successfully using LS-SA-PS hybrid optimization techniques. The computational results for the objective function respect to vagueness factor and level of satisfaction has been provided in the form of 2D and 3D plots. The outcome is very promising and strongly suggests that the hybrid LS-SA-PS algorithm is very efficient and productive in solving the large scale non-linear fuzzy programming problem.

  16. Semi-synthetic mithramycin SA derivatives with improved anticancer activity.

    PubMed

    Scott, Daniel; Chen, Jhong-Min; Bae, Younsoo; Rohr, Jürgen

    2013-05-01

    Mithramycin (MTM) is a potent anti-cancer agent that has recently garnered renewed attention. This manuscript describes the design and development of mithramycin derivatives through a combinational approach of biosynthetic analogue generation followed by synthetic manipulation for further derivatization. Mithramycin SA is a previously discovered analogue produced by the M7W1 mutant strain alongside the improved mithramycin analogues mithramycin SK and mithramycin SDK. Mithramycin SA shows decreased anti-cancer activity compared to mithramycin and has a shorter, two carbon aglycon side chain that is terminated in a carboxylic acid. The aglycon side chain is responsible for an interaction with the DNA-phosphate backbone as mithramycin interacts with its target DNA. It was therefore decided to further functionalize this side chain through reactions with the terminal carboxylic acid in an effort to enhance the interaction with the DNA phosphate backbone and improve the anti-cancer activity. This side chain was modified with a variety of molecules increasing the anti-cancer activity to a comparable level to mithramycin SK. This work shows the ability to transform the previously useless mithramycin SA into a valuable molecule and opens the door to further functionalization and semi-synthetic modification for the development of molecules with increased specificity and/or drug formulation. PMID:23331575

  17. NIPS-NORMA station-A combined facility for neutron-based nondestructive element analysis and imaging at the Budapest Neutron Centre

    NASA Astrophysics Data System (ADS)

    Kis, Zoltán; Szentmiklósi, László; Belgya, Tamás

    2015-04-01

    Neutron attenuation, scattering or radiative capture are used in various non-destructive methods to gain morphological, structural, elemental or isotopic information about the sample under study. The combined use of position-sensitive prompt gamma-ray detection (i.e. prompt gamma-ray activation imaging, PGAI) and neutron radiography/tomography (NR/NT) makes it possible to determine the 3D distribution of major elements and to visualize internal structures of heterogeneous objects in a non-destructive way. Based on earlier experience, the first ever permanent facility for this purpose, NIPS-NORMA, was constructed at the Budapest Neutron Centre, Hungary in 2012. The installation consists of a well-shielded, Compton-suppressed HPGe detector; a CCD-camera based imaging equipment and a motorized positioning system with sample support. Conventional PGAA measurements and NR/NT imaging using guided cold neutrons are the basic methods that form the basis of the more sophisticated experimental method called NR/NT-driven PGAI. The current status of the experimental station and its characteristics are described in the present paper.

  18. Computational studies of H5N1 hemagglutinin binding with SA-{alpha}-2, 3-Gal and SA-{alpha}-2, 6-Gal

    SciTech Connect

    Li Minyong; Wang Binghe . E-mail: wang@gsu.edu

    2006-09-01

    For influenza H5N1 hemagglutinin, a switch from SA-{alpha}-2, 3-Gal to SA-{alpha}-2, 6-Gal receptor specificity is a critical step leading to the conversion from avian-to-human to human-to-human infection. Therefore, the understanding of the binding modes of SA-{alpha}-2, 3-Gal and SA-{alpha}-2, 6-Gal to H5N1 hemagglutinin will be very important for the examination of possible mutations needed for going from an avian to a human flu virus. Based on the available H5N1 hemagglutinin crystal structure, the binding profiles between H5N1 hemagglutinin and two saccharide ligands, SA-{alpha}-2, 3-Gal and SA-{alpha}-2, 6-Gal, were investigated by ab initio quantum mechanics, molecular docking, molecular mechanics, and molecular dynamics simulations. It was found that SA-{alpha}-2, 3-Gal has strong multiple hydrophobic and hydrogen bond interactions in its trans conformation with H5N1 hemagglutinin, whereas the SA-{alpha}-2, 6-Gal only shows weak interactions in a different conformation (cis type)

  19. Level of Need for Cognition and Metacognitive Thinking among Undergraduate Kindergarten Female Students at King Sa'ud University in Sa'udi Arabia

    ERIC Educational Resources Information Center

    Daghistani, Bulquees

    2015-01-01

    This study aims at examining the level of need for cognition and metacognitive thinking among undergraduate kindergarten female students in Education Faculty at King Sa'ud University in Sa'udi Arabia from their own perceptions. Results showed that the need for the cognition level was moderate, but metacognitive thinking level was high. In…

  20. Salivaricin G32, a Homolog of the Prototype Streptococcus pyogenes Nisin-Like Lantibiotic SA-FF22, Produced by the Commensal Species Streptococcus salivarius.

    PubMed

    Wescombe, Philip A; Dyet, Kristin H; Dierksen, Karen P; Power, Daniel A; Jack, Ralph W; Burton, Jeremy P; Inglis, Megan A; Wescombe, Anna L; Tagg, John R

    2012-01-01

    Salivaricin G32, a 2667 Da novel member of the SA-FF22 cluster of lantibiotics, has been purified and characterized from Streptococcus salivarius strain G32. The inhibitory peptide differs from the Streptococcus pyogenes-produced SA-FF22 in the absence of lysine in position 2. The salivaricin G32 locus was widely distributed in BLIS-producing S. salivarius, with 6 (23%) of 26 strains PCR-positive for the structural gene, slnA. As for most other lantibiotics produced by S. salivarius, the salivaricin G32 locus can be megaplasmid encoded. Another member of the SA-FF22 family was detected in two Streptococcus dysgalactiae of bovine origin, an observation supportive of widespread distribution of this lantibiotic within the genus Streptococcus. Since the inhibitory spectrum of salivaricin G32 includes Streptococcus pyogenes, its production by S. salivarius, either as a member of the normal oral microflora or as a commercial probiotic, could serve to enhance protection of the human host against S. pyogenes infection. PMID:22567013

  1. Salivaricin G32, a Homolog of the Prototype Streptococcus pyogenes Nisin-Like Lantibiotic SA-FF22, Produced by the Commensal Species Streptococcus salivarius

    PubMed Central

    Wescombe, Philip A.; Dyet, Kristin H.; Dierksen, Karen P.; Power, Daniel A.; Jack, Ralph W.; Burton, Jeremy P.; Inglis, Megan A.; Wescombe, Anna L.; Tagg, John R.

    2012-01-01

    Salivaricin G32, a 2667 Da novel member of the SA-FF22 cluster of lantibiotics, has been purified and characterized from Streptococcus salivarius strain G32. The inhibitory peptide differs from the Streptococcus pyogenes—produced SA-FF22 in the absence of lysine in position 2. The salivaricin G32 locus was widely distributed in BLIS-producing S. salivarius, with 6 (23%) of 26 strains PCR-positive for the structural gene, slnA. As for most other lantibiotics produced by S. salivarius, the salivaricin G32 locus can be megaplasmid encoded. Another member of the SA-FF22 family was detected in two Streptococcus dysgalactiae of bovine origin, an observation supportive of widespread distribution of this lantibiotic within the genus Streptococcus. Since the inhibitory spectrum of salivaricin G32 includes Streptococcus pyogenes, its production by S. salivarius, either as a member of the normal oral microflora or as a commercial probiotic, could serve to enhance protection of the human host against S. pyogenes infection. PMID:22567013

  2. The visualization of DHSI based on the relationship model of CL and SA

    NASA Astrophysics Data System (ADS)

    Wang, Haibo; Guo, Huijuan; Xue, Chengqi

    2013-03-01

    The source and cause of cognitive load (CL) and situation awareness (SA) in digital human-system interface (DHSI) is explained. Based on the analysis of cognitive procedure and the relation of CL and SA, The relationship model of CL and SA in DHSI is put forward. Using the association target and the relationship model of CL and SA, the method and strategy of information visualization of DHSI is presented which is based on the cognitive psychology. Selecting the Boeing 757 autopilot as the case, the comparison of design is shown.

  3. Abundant Molecular Gas and Inefficient Star Formation in Intracluster Regions: Ram Pressure Stripped Tail of the Norma Galaxy ESO137-001

    NASA Astrophysics Data System (ADS)

    Jáchym, Pavel; Combes, Françoise; Cortese, Luca; Sun, Ming; Kenney, Jeffrey D. P.

    2014-09-01

    For the first time, we reveal large amounts of cold molecular gas in a ram-pressure-stripped tail, out to a large "intracluster" distance from the galaxy. With the Actama Pathfinder EXperiment (APEX) telescope, we have detected 12CO(2-1) emission corresponding to more than 109 M ⊙ of H2 in three Hα bright regions along the tail of the Norma cluster galaxy ESO 137-001, out to a projected distance of 40 kpc from the disk. ESO 137-001 has an 80 kpc long and bright X-ray tail associated with a shorter (40 kpc) and broader tail of numerous star forming H II regions. The amount of ~1.5 × 108 M ⊙ of H2 found in the most distant region is similar to molecular masses of tidal dwarf galaxies, though the standard Galactic CO-to-H2 factor could overestimate the H2 content. Along the tail, we find the amount of molecular gas to drop, while masses of the X-ray-emitting and diffuse ionized components stay roughly constant. Moreover, the amounts of hot and cold gas are large and similar, and together nearly account for the missing gas from the disk. We find a very low SFE (τdep > 1010 yr) in the stripped gas in ESO 137-001 and suggest that this is due to a low average gas density in the tail, or turbulent heating of the interstellar medium that is induced by a ram pressure shock. The unprecedented bulk of observed H2 in the ESO 137-001 tail suggests that some stripped gas may survive ram pressure stripping in the molecular phase. Based on observations made with ESO telescopes at La Silla Paranal Observatory under program ID 088.B-0934.

  4. NEW YOUNG STAR CANDIDATES IN CG4 AND Sa101

    SciTech Connect

    Rebull, L. M.; Laine, S.; Laher, R.; Legassie, M.; Hoette, V.; Kim, J. S.; Foster, M.; Mallory, C. R.; McCarron, K.; Sherry, W. H.

    2011-07-15

    The CG4 and Sa101 regions together cover a region of {approx}0.5 deg{sup 2} in the vicinity of a 'cometary globule' that is part of the Gum Nebula. There are seven previously identified young stars in this region; we have searched for new young stars using mid- and far-infrared data (3.6-70 {mu}m) from the Spitzer Space Telescope, combined with ground-based optical data and near-infrared data from the Two Micron All Sky Survey. We find infrared excesses in all six of the previously identified young stars in our maps and identify 16 more candidate young stars based on apparent infrared excesses. Most (73%) of the new young stars are Class II objects. There is a tighter grouping of young stars and young star candidates in the Sa101 region, in contrast to the CG4 region, where there are fewer young stars and young star candidates, and they are more dispersed. Few likely young objects are found in the 'fingers' of the dust being disturbed by the ionization front from the heart of the Gum Nebula.

  5. Finite element analysis of (SA) mechanoreceptors in tactile sensing application

    NASA Astrophysics Data System (ADS)

    N, Syamimi; Yahud, S.

    2015-05-01

    This paper addresses the structural design of a fingertip model in order to analyse the sensory function of slow adapting (SA) mechanoreceptors by using the finite element analysis (FEA) method. A biologically inspired tactile sensor was designed to mimic a similar response of the human mechanoreceptors in the human glabrous skin. The simulation work was done by using COMSOL Multiphysics. The artificial skin was modelled as a solid square block of silicone elastomer with a semi cylinder protrusion on top. It was modelled as a nearly incompressible and linear hyperelastic material defined by Neo Hookean constitutive law. The sensing element on the other hand was modelled by using constantan alloy mimicking the SA1 receptor. Boundary loads of 1 N/m² to 4 N/m² with the increment of 1 N/m² were applied to the top surface of the protrusion in z and x-direction for normal and shear stress, respectively. The epidermal model base was constrained to maintain the same boundary conditions throughout all simulations. The changes of length experienced by the sensing element were calculated. The simulations result in terms of strain was identified. The simulated result was plotted in terms of sensing element strain against the boundary load and the graph should produce a linear response.

  6. Draft Genome Sequence of Rheinheimera sp. Strain SA_1 Isolated from Iron Backwash Sludge in Germany.

    PubMed

    Schröder, Josephin; Braun, Burga; Liere, Karsten; Szewzyk, Ulrich

    2016-01-01

    Rheinheimera sp. strain SA_1 is an iron-depositing bacterium for which we report a draft genome sequence. Strain SA_1 was isolated from iron backwash sludge of a waterworks in Germany. The Illumina MiSeq technique was used to sequence the genome of the strain. PMID:27540074

  7. 76 FR 7511 - Airworthiness Directives; Empresa Brasileira de Aeronautica S.A. (EMBRAER) Model ERJ 170...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will... Directives; Empresa Brasileira de Aeronautica S.A. (EMBRAER) Model ERJ 170 Airplanes; and Model ERJ 190-100..., contact Empresa Brasileira de Aeronautica S.A. (EMBRAER), Technical Publications Section (PC 060),...

  8. Sequence Determinants for DNA Packaging Specificity in the S. aureus Pathogenicity Island SaPI1

    PubMed Central

    Bento, Joana C; Lane, Kristin D; Read, Erik K; Cerca, Nuno; Christie, Gail E

    2014-01-01

    The SaPIs and their relatives are a family of genomic islands that exploit helper phages for high frequency horizontal transfer. One of the mechanisms used by SaPIs to accomplish this molecular piracy is the redirection of the helper phage DNA packaging machinery. SaPIs encode a small terminase subunit that can be substituted for that of the phage. In this study we have determined the initial packaging cleavage sites for helper phage 80α which uses the phage-encoded small terminase subunit, and for SaPI1, which uses the SaPI-encoded small terminase subunit. We have identified a 19 nt SaPI1 sequence that is necessary and sufficient to allow high frequency 80α transduction of a plasmid by a terminase carrying the SaPI1-encoded small subunit. We also show that the hybrid enzyme with the SaPI1 small terminase subunit is capable of generalized transduction. PMID:24365721

  9. Sequence determinants for DNA packaging specificity in the S. aureus pathogenicity island SaPI1.

    PubMed

    Bento, Joana C; Lane, Kristin D; Read, Erik K; Cerca, Nuno; Christie, Gail E

    2014-01-01

    The SaPIs and their relatives are a family of genomic islands that exploit helper phages for high frequency horizontal transfer. One of the mechanisms used by SaPIs to accomplish this molecular piracy is the redirection of the helper phage DNA packaging machinery. SaPIs encode a small terminase subunit that can be substituted for that of the phage. In this study we have determined the initial packaging cleavage sites for helper phage 80α, which uses the phage-encoded small terminase subunit, and for SaPI1, which uses the SaPI-encoded small terminase subunit. We have identified a 19nt SaPI1 sequence that is necessary and sufficient to allow high frequency 80α transduction of a plasmid by a terminase carrying the SaPI1-encoded small subunit. We also show that the hybrid enzyme with the SaPI1 small terminase subunit is capable of generalized transduction. PMID:24365721

  10. Draft Genome Sequence of Rheinheimera sp. Strain SA_1 Isolated from Iron Backwash Sludge in Germany

    PubMed Central

    Schröder, Josephin; Liere, Karsten; Szewzyk, Ulrich

    2016-01-01

    Rheinheimera sp. strain SA_1 is an iron-depositing bacterium for which we report a draft genome sequence. Strain SA_1 was isolated from iron backwash sludge of a waterworks in Germany. The Illumina MiSeq technique was used to sequence the genome of the strain. PMID:27540074

  11. Best Practices Case Study: S&A Homes, East Liberty, PIttsburgh, PA

    SciTech Connect

    2010-12-01

    S&A Homes worked with Building America's IBACOS and architects Pfaffmann & Associates and Moss Associates to design energy-efficient homes for urban in-fill lots. This is a new market for S&A Homes, which builds over 500 homes a year using suburban designs.

  12. Characteristics and development report for the SA3871 Intent Controller application specific integrated circuit (ASIC)

    SciTech Connect

    Simpson, R.L.; Meyer, B.T.

    1995-08-01

    This report describes the design and development activities that were involved in the SA3871 Intent Controller ASIC. The SA3871 is a digital gate array component developed for the MC4396 Trajectory Sensing Signal Generator for use in the B61-3/4/10 system as well as a possible future B61-MAST system.

  13. SEU characterization and design dependence of the SA3300 microprocessor

    SciTech Connect

    Sexton, F.W.; Treece, R.K.; Hass, K.J.; Hash, G.L.; Axness, C.L. ); Hughes, K.L. ); Buchner, S.P.; Kang, K. )

    1990-12-01

    The SEU vulnerability of the SA3300 16-bit microprocessor has been characterized, and the effects of two different design revisions on error rate have been explored. The authors have found that the threshold for upset depends on the data pattern written into the general purpose registers. With all bits in the general purpose registers set to logic one, a design with 2-{mu}m n- and p-channel transistor lengths had a threshold LET of 35 MeV-cm{sup 2}/mg at 25{degrees} C and 4.5 volt operation. With all zero's stored in the registers the upset threshold increased by more than a factor of two to 83 MeV- cm{sup 2}/mg. A second design revision, with 1.25-{mu}m and 1.75-{mu}m n- and p-channel transistor lengths, respectively, was more vulnerable to upset, but exhibited a smaller dependence on logic state.

  14. Cryogenic requirements for the JT-60SA Tokamak

    NASA Astrophysics Data System (ADS)

    Michel, Frederic; Hitz, D.; Hoa, Christine; Lamaison, Valerie; Kamiya, Koji; Roussel, Pascal; Wanner, Manfred; Yoshida, Kiyoshi

    2012-06-01

    The superconducting tokamak JT-60SA is part of the Broader Approach Programmeagreed between Japan and Europe. CEA is in charge of the cryogenic system procurementincluding the Warm Compression Station, the gas storages, the Refrigerator Cold Box andthe Auxiliary Cold Box (ACB) which has to be installed on the JAEA Naka site in 2016.This paper summarizes the updated cryogenic requirements for the tokamak JT-60SAcryogenic system. The cryogenic system has a refrigeration capacity of about 9 kW equivalent at 4.5K, to supply cryopump panels at 3.7 K, superconducting magnets and cold structures at 4.4 K, HTS current leads at 50 K, and thermal shields at 80 K. This paper presents the static and variable heat loads of the different cooling loops and the results of the rmohydraulic calculations to derive the transient heat loads at the interface between the magnet system cooling loops and the Auxiliary Cold Box.

  15. SalSA: A Teraton UHE Neutrino Detector

    SciTech Connect

    Reil, Kevin; /SLAC

    2006-04-19

    The observed spectrum of ultra-high energy cosmic rays virtually guarantees the presence of ultra-high energy neutrinos due to their interaction with the cosmic microwave background. Unlike cosmic rays, each of these neutrinos will point back directly to its source and will arrive at the Earth unattenuated, from sources perhaps as distant as z = 20. The neutrino telescopes currently under construction, should discover a handful of these events, probably too few for detailed study. This paper describes how an array of VHF and UHF antennas embedded in a large salt dome, SalSA (Salt dome Shower Array) promises to yield a teraton detector (> 500 km{sup 3 sr}) for contained neutrino events with energies above 10{sup 17} eV. Our simulations show that such a detector may observe several hundreds of these neutrinos over its lifetime with excellent angular resolution providing source locations.

  16. Performance analysis of SA-3 missile second stage

    NASA Technical Reports Server (NTRS)

    Helmy, A. M.

    1981-01-01

    One SA-3 missile was disassembled. The constituents of the second stage were thoroughly investigated for geometrical details. The second stage slotted composite propellant grain was subjected to mechanical properties testing, physiochemical analyses, and burning rate measurements at different conditions. To determine the propellant performance parameters, the slotted composite propellant grain was machined into a set of small-size tubular grains. These grains were fired in a small size rocket motor with a set of interchangeable nozzles with different throat diameters. The firings were carried out at three different conditions. The data from test motor firings, physiochemical properties of the propellant, burning rate measurement results and geometrical details of the second stage motor, were used as input data in a computer program to compute the internal ballistic characteristics of the second stage.

  17. Editorial challenges of the Revista de Saúde Pública.

    PubMed

    Antunes, José Leopoldo Ferreira; França Júnior, Ivan; de Andrade, Maria Teresinha Dias; Barata, Rita de Cássia Barradas; Monteiro, Carlos Augusto

    2015-07-01

    The editors of the Revista de Saúde Pública describe the journal's editorial profile and discuss the challenges of scientific publication in the area. A historical overview of almost 50 years of the journal is reported, with the temporal projection of their bibliometric indicators. Qualitative and quantitative parameters of its editorial profile and indexes are also reported. Budget constraints and the actions being taken to address them are discussed. The difficulty in allocating reviewers for manuscripts submitted to the editorial process is also discussed; an issue that affects scientific publication in many areas of knowledge. In particular, we sought to reflect on the proposal of measures to boost the editorial process by alleviating the shortage of reviewers and their possible harmful consequences for the editorial process. PMID:26132238

  18. SA1 and TRF1 synergistically bind to telomeric DNA and promote DNA-DNA pairing

    NASA Astrophysics Data System (ADS)

    Wang, Hong; Lin, Jiangguo; Countryman, Preston; Pan, Hai; Parminder Kaur Team; Robert Riehn Team; Patricia Opresko Team; Jane Tao Team; Susan Smith Team

    Impaired telomere cohesion leads to increased aneuploidy and early onset of tumorigenesis. Cohesion is thought to occur through the entrapment of two DNA strands within tripartite cohesin ring(s), along with a fourth subunit (SA1/SA2). Surprisingly, cohesion rings are not essential for telomere cohesion, which instead requires SA1 and shelterin proteins including TRF1. However, neither this unique cohesion mechanism at telomeres or DNA-binding properties of SA1 is understood. Here, using single-molecule fluorescence imaging of quantum dot-labeled proteins on DNA we discover that while SA1 diffuses across multiple telomeric and non-telomeric regions, the diffusion mediated through its N-terminal domain is slower at telomeric regions. However, addition of TRF1 traps SA1 within telomeric regions, which form longer DNA-DNA pairing tracts than with TRF1 alone, as revealed by atomic force microscopy. Together, these experimental results and coarse-grained molecular dynamics simulations suggest that TRF1 and SA1 synergistically interact with DNA to support telomere cohesion without cohesin rings.

  19. Photoelectric UVBY beta photometry of 230 stars brighter than m(pg) = 13.0 in the two B = +75 deg fields SA 80 and SA 81

    NASA Astrophysics Data System (ADS)

    Knude, J.

    1993-04-01

    The study presents uvby and beta photometry for 123 and 107 stars in SA 80 and SA 81 brighter than m(pg) = 13 and earlier than G1. Most stars seem to be of type A or F with sample limits V is not greater than 12.6 and beta is not less than 2.60. SA 80 and SA 81 have 34 and 27 BD stars in common with a general b is not less than 70 deg program, respectively. uvby photometry for 19 and 12 stars fainter than m(pg) = 13 is also presented. If situated on the standard line, the sample of absolutely less luminous F9-G0 stars are complete in the volume confined to the distance range from 80 to 300-400 pc. Several lines of sight have E(b-y) exceeding 0.050 m. In both areas the remote A stars seem to be virtually unreddened or are perhaps bluer than typical for population I stars. SA 80 covers part of the Virgo cluster, and the data may be used to estimate the galactic reddening in this direction.

  20. Cryogenic thermometry for refrigerant distribution system of JT-60SA

    NASA Astrophysics Data System (ADS)

    Natsume, K.; Murakami, H.; Kizu, K.; Yoshida, K.; Koide, Y.

    2015-12-01

    JT-60SA is a fully superconducting fusion experimental device involving Japan and Europe. The cryogenic system supplies supercritical or gaseous helium to superconducting coils through valve boxes or coil terminal boxes and in-cryostat pipes. There are 86 temperature measurement points at 4 K along the distribution line. Resistance temperature sensors will be installed on cooling pipes in vacuum. In this work, two sensor attachment methods, two types of sensor, two thermal anchoring methods, and two sensor fixation materials have been experimentally evaluated in terms of accuracy and mass productivity. Finally, the verification test of thermometry has been conducted using the sample pipe fabricated in the same way to the production version, which has been decided by the comparison experiments. The TVO sensor is attached by the saddle method with Apiezon N grease and the measurement wires made of phosphor bronze are wound on the pipe with Stycast 2850FT as the thermal anchoring. A Cernox sensor is directly immersed in liquid helium as a reference thermometer during the experiment. The measured temperature difference between the attached one and reference one has been within ±15 mK in the range of 3.40-4.73 K. It has satisfies the accuracy requirement of 0.1 K.

  1. SA1 binds directly to DNA through its unique AT-hook to promote sister chromatid cohesion at telomeres

    PubMed Central

    Bisht, Kamlesh K.; Daniloski, Zharko; Smith, Susan

    2013-01-01

    Summary Sister chromatid cohesion relies on cohesin, a complex comprising a tri-partite ring and a peripheral subunit Scc3, which is found as two related isoforms SA1 and SA2 in vertebrates. There is a division of labor between the vertebrate cohesin complexes; SA1-cohesin is required at telomeres and SA2-cohesin at centromeres. Depletion of SA1 has dramatic consequences for telomere function and genome integrity, but the mechanism by which SA1-cohesin mediates cohesion at telomeres is not well understood. Here we dissect the individual contribution of SA1 and the ring subunits to telomere cohesion and show that telomeres rely heavily on SA1 and to a lesser extent on the ring for cohesion. Using chromatin immunoprecipitation we show that SA1 is highly enriched at telomeres, is decreased at mitosis when cohesion is resolved, and is increased when cohesion persists. Overexpression of SA1 alone was sufficient to induce cohesion at telomeres, independent of the cohesin ring and dependent on its unique (not found in SA2) N-terminal domain, which we show binds to telomeric DNA through an AT-hook motif. We suggest that a specialized cohesion mechanism may be required to accommodate the high level of DNA replication-associated repair at telomeres. PMID:23729739

  2. Functional interplay between SA1 and TRF1 in telomeric DNA binding and DNA-DNA pairing.

    PubMed

    Lin, Jiangguo; Countryman, Preston; Chen, Haijiang; Pan, Hai; Fan, Yanlin; Jiang, Yunyun; Kaur, Parminder; Miao, Wang; Gurgel, Gisele; You, Changjiang; Piehler, Jacob; Kad, Neil M; Riehn, Robert; Opresko, Patricia L; Smith, Susan; Tao, Yizhi Jane; Wang, Hong

    2016-07-27

    Proper chromosome alignment and segregation during mitosis depend on cohesion between sister chromatids. Cohesion is thought to occur through the entrapment of DNA within the tripartite ring (Smc1, Smc3 and Rad21) with enforcement from a fourth subunit (SA1/SA2). Surprisingly, cohesin rings do not play a major role in sister telomere cohesion. Instead, this role is replaced by SA1 and telomere binding proteins (TRF1 and TIN2). Neither the DNA binding property of SA1 nor this unique telomere cohesion mechanism is understood. Here, using single-molecule fluorescence imaging, we discover that SA1 displays two-state binding on DNA: searching by one-dimensional (1D) free diffusion versus recognition through subdiffusive sliding at telomeric regions. The AT-hook motif in SA1 plays dual roles in modulating non-specific DNA binding and subdiffusive dynamics over telomeric regions. TRF1 tethers SA1 within telomeric regions that SA1 transiently interacts with. SA1 and TRF1 together form longer DNA-DNA pairing tracts than with TRF1 alone, as revealed by atomic force microscopy imaging. These results suggest that at telomeres cohesion relies on the molecular interplay between TRF1 and SA1 to promote DNA-DNA pairing, while along chromosomal arms the core cohesin assembly might also depend on SA1 1D diffusion on DNA and sequence-specific DNA binding. PMID:27298259

  3. Does Salicylic Acid (SA) Improve Tolerance to Salt Stress in Plants? A Study of SA Effects On Tomato Plant Growth, Water Dynamics, Photosynthesis, and Biochemical Parameters.

    PubMed

    Mimouni, Hajer; Wasti, Salma; Manaa, Arafet; Gharbi, Emna; Chalh, Abdellah; Vandoorne, Bertrand; Lutts, Stanley; Ben Ahmed, Hela

    2016-03-01

    Environmental stresses such as salinity directly impact crop growth, and by extension, world food supply and societal prosperity. It is estimated that over 800 million hectares of land throughout the world are salt-affected. In arid and semi-arid regions, salt concentration can be close to that in the seawater. Hence, there are intensive efforts to improve plant tolerance to salinity and other environmental stressors. Salicylic acid (SA) is an important signal molecule for modulating plant responses to stress. In the present study, we examined, on multiple plant growth related endpoints, whether SA applied through the rooting medium could mitigate the adverse effects of salinity on tomato (Solanum lycopersicum) cv. Marmande. The latter is a hitherto understudied tomato plant from the above perspective; it is a classic variety that produces the large ribbed tomatoes in the Mediterranean and consumed worldwide. We found salt stress negatively affected the growth of cv. Marmande tomato plants. However, the SA-treated plants had greater shoot and root dry mass, leaf area compared to untreated plants when exposed to salt stress. Application of SA restores photosynthetic rates and photosynthetic pigment levels under salt (NaCl) exposure. Leaf water, osmotic potential, stomatal conductance transpiration rate, and biochemical parameters were also ameliorated in SA-treated plants under saline stress conditions. Overall, these data illustrate that SA increases cv. Marmande tomato growth by improving photosynthesis, regulation and balance of osmotic potential, induction of compatible osmolyte metabolism, and alleviating membrane damage. We suggest salicylic acid might be considered as a potential growth regulator to improve tomato plant salinity stress resistance, in the current era of global climate change. PMID:26909467

  4. Gemini surfactants affect the structure, stability, and activity of ribonuclease Sa.

    PubMed

    Amiri, Razieh; Bordbar, Abdol-Khalegh; Laurents, Douglas V

    2014-09-11

    Gemini surfactants have important advantages, e.g., low micromolar CMCs and slow millisecond monomer ↔ micelle kinetics, for membrane mimetics and for delivering nucleic acids for gene therapy or RNA silencing. However, as a prerequisite, it is important to characterize interactions occurring between Gemini surfactants and proteins. Here NMR and CD spectroscopies are employed to investigate the interactions of cationic Gemini surfactants with RNase Sa, a negatively charged ribonuclease. We find that RNase Sa binds Gemini surfactant monomers and micelles at pH values above 4 to form aggregates. Below pH 4, where the protein is positively charged, these aggregates dissolve and interactions are undetectable. Thermal denaturation experiments show that surfactant lowers RNase Sa's conformational stability, suggesting that surfactant binds the protein's denatured state preferentially. Finally, Gemini surfactants were found to bind RNA, leading to the formation of large complexes. Interestingly, Gemini surfactant binding did not prevent RNase Sa from cleaving RNA. PMID:25133582

  5. 76 FR 72855 - Airworthiness Directives; Empresa Brasileira de Aeronautica S.A. (EMBRAER) Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or... Brasileira de Aeronautica S.A. (EMBRAER), Technical Publications Section (PC 060), Av. Brigadeiro Faria...

  6. Anti-Sa antibodies: prognostic and pathogenetic significance to rheumatoid arthritis

    PubMed Central

    El-Gabalawy, Hani S; Wilkins, John A

    2004-01-01

    Anti-Sa antibodies are detected in the serum of 20–47% of patients with rheumatoid arthritis. These antibodies have a high degree of specificity for the disease, and appear to identify a subset of early rheumatoid arthritis patients destined to have aggressive and destructive disease. It has recently been confirmed that anti-Sa antibodies are directed to citrullinated vimentin, thus placing them in the anti-citrulline family of autoantibodies. The Sa antigen has previously been shown to be present in synovium. This, along with the demonstration of citrullinated proteins in rheumatoid synovium, suggests that anti-Sa antibodies may play a pathogenetic role in the initiation and/or persistence of rheumatoid synovitis. PMID:15059270

  7. 78 FR 68697 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-15

    ..., Amendment 39-17321 (78 FR 6725, January 31, 2013), and adding the following new AD: 2013-22-22 Turbomeca S.A...-17321 (78 FR 6725, January 31, 2013). (c) Applicability This AD applies to all Turbomeca S.A. Arriel 2D...-07, Amendment 39-17321 (78 FR 6725, January 31, 2013), (``AD 2013-01-07''). AD 2013-01-07 applied...

  8. The mealybug Phenacoccus solenopsis suppresses plant defense responses by manipulating JA-SA crosstalk

    PubMed Central

    Zhang, Peng-Jun; Huang, Fang; Zhang, Jin-Ming; Wei, Jia-Ning; Lu, Yao-Bin

    2015-01-01

    Induced plant defenses against herbivores are modulated by jasmonic acid-, salicylic acid-, and ethylene-signaling pathways. Although there is evidence that some pathogens suppress plant defenses by interfering with the crosstalk between different signaling pathways, such evidence is scarce for herbivores. Here, we demonstrate that the mealybug Phenacoccus solenopsis suppresses the induced defenses in tomato. We found that exogenous JA, but not SA, significantly decreased mealybug feeding time and reduced nymphal performance. In addition, constitutive activation of JA signaling in 35s::prosys plants reduced mealybug survival. These data indicate that the JA signaling pathway plays a key role in mediating the defense responses against P. solenopsis. We also found that mealybug feeding decreased JA production and JA-dependent defense gene expression, but increased SA accumulation and SA-dependent gene expression. In SA-deficient plants, mealybug feeding did not suppress but activated JA accumulation, indicating that the suppression of JA-regulated defenses depends on the SA signaling pathway. Mealybugs benefit from suppression of JA-regulated defenses by exhibiting enhanced nymphal performance. These findings confirm that P. solenopsis manipulates plants for its own benefits by modulating the JA-SA crosstalk and thereby suppressing induced defenses. PMID:25790868

  9. Closed-loop control of SaO2 in the neonate.

    PubMed

    Morozoff, P E; Evans, R W

    1992-01-01

    A microprocessor-based device has been designed to control oxygen saturation (SaO2) in neonates by adjusting the inspired air-oxygen mixture (FiO2) delivered by a mechanical blender. The user sets a target SaO2, which the controller attempts to maintain. Alarms are actuated if the neonate's SaO2 is outside predefined limits. SaO2 levels are extracted from a commercial pulse oximeter and analyzed by an eight-bit microprocessing unit (MPU). Delivered percentages of FiO2 are adjusted by a motorized air-oxygen blender. The controller has a menu-driven user interface and can graphically present four-hour trends of the SaO2, FiO2, or blender setting. Sixteen hours of collected data can be stored and later downloaded to a personal computer. A real-time multitasking operating system forms the nucleus of the controller's software. Major tasks that share MPU time are control, filtering, user display, data collection, data archiving, alarm monitoring, and user input. Analog SaO2 levels are read and converted to digital values, which are then filtered to extract noise. A differential control algorithm is used to determine the required FiO2 blender setting. The blender is then adjusted to the new setting, after which the controller waits to repeat the process of sampling SaO2 and adjusting FiO2. System response time and blender increments are adjustable to allow a user to tune the controller to the patient's needs. Alarm conditions of concern within the device are SaO2 and FiO2 sensor disconnection, blender disconnection, and SaO2 limiting errors. In preliminary trials, for a target of 92.0% SaO2, a prototype controller maintained an average of 91.6% with a standard deviation of 5.0% over a one-hour period.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1562825

  10. Performance of SaSelect, a Chromogenic Medium for Detection of Staphylococci in Clinical Specimens

    PubMed Central

    Kerttula, Anne-Marie; Kaukoranta, Suvi-Sirkku

    2014-01-01

    In a preliminary study, known staphylococcus (n = 86) and other microbial (n = 12) isolates were plated on three chromogenic media, SaSelect (Bio-Rad, Hercules, CA, USA), CHROMagar Staph. aureus (CHROMagar Microbiology, Paris, France), and S. aureus ID (bioMérieux, Marcy l'Etoile, France). The sensitivities of all the media to detect Staphylococcus aureus after 24 h of incubation were high (100.0%). However, their specificities varied at 93.3% (95% confidence interval [CI], 86.0% to 100.0%) (CHROMagar Staph. aureus), 97.8% (95% CI, 93.5% to 100.0%) (S. aureus ID), and 100.0% (SaSelect). SaSelect also showed the highest sensitivity for recovery and differentiation of other staphylococci. As the best performing chromogenic medium, SaSelect was then prospectively compared to conventional culture and identification tests for the detection of staphylococci from 2,780 clinical specimens. A total of 1,589 staphylococcal isolates were recovered. Of these, 912 were S. aureus and 677 were other staphylococci. The sensitivity and specificity of SaSelect to detect S. aureus in clinical specimens after 24 h of incubation were 99.6% and 99.9% (95% CI, 99.2% to 100.0% and 99.8% to 100.0%), respectively, whereas the sensitivity and specificity using conventional plates combined with laboratory identification methods were 96.8% and 99.5% (95% CI, 95.7 to 97.9% and 99.2% to 99.8%). For the recovery and preliminary identification of other staphylococci, the sensitivity and specificity of SaSelect were 94.4% and 99.9%. SaSelect is a well-performing chromogenic medium that significantly improved the detection of staphylococci, especially S. aureus, compared to conventional culture (P < 0.0001). PMID:24430448

  11. Henrique da Rocha Lima.

    PubMed

    Bernardes Filho, Fred; Avelleira, João Carlos Regazzi

    2015-01-01

    Brazilian physician and researcher Henrique da Rocha Lima was born in 1879 in the city of Rio de Janeiro, where he studied medicine and obtained the degree of M.D. in 1901. He specialized in Clinical Medicine in Germany and was the ambassador in European countries of the scientific medicine that emerged from the Oswaldo Cruz Institute in the early twentieth century. Rocha Lima has discovered the causative agent of typhus and had a major contribution to the studies of yellow fever, Chagas disease, Carrión's disease and histoplasmosis. His genius, his research and his discoveries projected his name, and, with it, the image of Brazil in the international scientific scene. PMID:26131867

  12. Henrique da Rocha Lima*

    PubMed Central

    Bernardes Filho, Fred; Avelleira, João Carlos Regazzi

    2015-01-01

    Brazilian physician and researcher Henrique da Rocha Lima was born in 1879 in the city of Rio de Janeiro, where he studied medicine and obtained the degree of M.D. in 1901. He specialized in Clinical Medicine in Germany and was the ambassador in European countries of the scientific medicine that emerged from the Oswaldo Cruz Institute in the early twentieth century. Rocha Lima has discovered the causative agent of typhus and had a major contribution to the studies of yellow fever, Chagas disease, Carrión’s disease and histoplasmosis. His genius, his research and his discoveries projected his name, and, with it, the image of Brazil in the international scientific scene. PMID:26131867

  13. Antibody-dependent enhancement of dengue virus infection is inhibited by SA-17, a doxorubicin derivative.

    PubMed

    Ayala-Nuñez, Nilda V; Jarupathirun, Patsaporn; Kaptein, Suzanne J F; Neyts, Johan; Smit, Jolanda M

    2013-10-01

    Antibody-dependent enhancement (ADE) is thought to play a critical role in the exacerbation of dengue virus (DENV)-induced disease during a heterologous re-infection. Despite ADE's clinical impact, only a few antiviral compounds have been assessed for their anti-ADE activity. We reported earlier that SA-17, a doxorubicin derivative, efficiently inhibits the in vitro infection of DENV and yellow fever virus. Here we explored SA-17's mechanism of inhibition and investigated if the compound is active against ADE of DENV infection. Since enhanced infectivity stimulated by antibodies has been observed with standard and immature DENV, both types of virions were included in the study. We observed that SA-17 (i) inhibits DENV infection by preventing binding/entry to the cell and (ii) interferes with antibody-mediated infection of both standard and immature DENV2. SA-17 markedly reduced the infectivity of DENV2 in ADE conditions, with IC50s ranging from 0.26 to 2.89μM. The compound exerted its activity when added before, during, and after antibody-opsonization of standard and immature virus. Thus, molecules with the characteristics of SA-17 may be attractive antiviral agents since they can be used both to block DENV2 entry during primary and secondary infection and to inhibit ADE of standard and immature virus. PMID:23994499

  14. Comparison of finite element analysis of a piping tee using NASTRAN and CORTES/SA

    NASA Technical Reports Server (NTRS)

    Quezon, A. J.; Everstine, G. C.

    1980-01-01

    A comparison of finite element analyses of a piping tee was made using NASTRAN and CORTES/SA, a modified version of SAP3 having a special purpose input processor for generating geometries for a wide variety of tee joints. Four finite element models were subjected in force, moment, and pressure loadings. Flexibility factors and principal stresses were computed for each model and compared with results obtained experimentally by Combustion Engineering, Inc. Results from the NASTRAN analyses were in good agreement with experimental results for all loadings except internal pressure. The CORTES/SA analyses gave good results for the internal pressure loading, but poorer results for out of plane bending moments or forces resulting in out of plane bending. Two of the basic load cases in CORTES/SA were found to contain errors that could not be easily corrected. COST COMPARison of NASTRAN and CORTES/SA showed NASTRAN to be less expensive to two than CORTES/SA for identical meshes.

  15. Evaluation of the Quantitative Dry Culture Method (Sanitakun(TM) SA) for the Enumeration of Staphylococcus aureus in Artificially Contaminated Food Samples.

    PubMed

    Teramura, Hajime; Iwasaki, Mihoko; Ogihara, Hirokazu

    2015-01-01

    Sanita-kun(TM) SA for Staphylococcus aureus (SkSA), a novel dry sheet quantitative culture system, was evaluated. When the inclusivity and exclusivity of SkSA were assessed using 121 microorganisms including 47 S. aureus strains, the tested S. aureus strains formed blue-colored colonies on the SkSA and all the other microbes failed to grow. The SkSA was then compared with Baird-Parker agar (BP) according to ISO 6888-1, Mannitol salt agar with egg yolk (MSEY), and 3M Petrifilm(TM) STX (3M-STX) in 100 artificially contami nated food samples. The correlation coefficients between SkSA and BP, SkSA and MSEY, and SkSA and 3M-STX were 0.971, 0.989 and 0.996, respectively. Our results demonstrated that SkSA is a suitable alternative for the enumeration of S. aureus in foods. PMID:26699862

  16. Grating Ti:Sa laser: Rydberg & auto-ionizing state spectroscopy

    NASA Astrophysics Data System (ADS)

    Teigelhoefer, Andrea; Bricault, Pierre; Lassen, Jens; Neu, Walter; Wendt, Klaus

    2009-05-01

    TRIUMF's Isotope Separator and Accelerator facility (ISAC) provides intense radioactive isotope beams (RIB) for nuclear and particle physics experiments. Resonant laser ionization is well suited as an on-line ion source for RIB production due to its efficiency and element selectivity. TRIUMF's Laser Ion Source (TRI LIS) uses BRF tuned Ti:Sa lasers with GHz linewidth and 10kHz rep. rate. Continuous wavelength scanning of these lasers is involved. A grating tuned Ti:Sa laser was built to allow for high resolution continuous wavelength scans (10nm/h) thus allowing for systematic studies of high lying atomic energy levels and the development of efficient RIS schemes. This grating tuned Ti:Sa laser system will be presented.

  17. ASTP (SA-210) launch vehicle operational flight trajectory dispersion analysis, volume 1

    NASA Technical Reports Server (NTRS)

    Williams, N. D.; Klug, G. W.; Ransom, F. A.

    1975-01-01

    In order to establish realistic deviation limits for the ASTP (SA-210) Launch Vehicle Operational Flight Trajectory, a dispersion analysis was conducted. The nominal trajectory prescribed for this analysis is the ASTP (SA-210) Launch Vehicle 500 Pound Launch Window Opening OT. The error sources considered are those associated with predictions of vehicle characteristics, vehicle systems performance, and flight environment. The nominal vehicle, the boost trajectory simulations, the error sources, the analytic procedures utilized, and the results are discussed. Launch vehicle guidance system inaccuracies were determined from the guidance error analysis. These data are composed of individual error source trajectory parameter dispersion envelopes.

  18. Minimization of Reactive Power Fluctuation in JT-60SA Magnet Power Supply

    NASA Astrophysics Data System (ADS)

    Shimada, K.; Terakado, T.; Yamauchi, K.; Matsukawa, M.; Baulaigue, O.; Coletti, R.; Coletti, A.; Novello, L.

    2013-02-01

    This paper describes an asymmetric control method for the firing angle and a start/stop timing shift control of four thyristor converters called “Booster PS" to minimize the reactive power fluctuation during plasma initiation in JT-60SA. From the simulation using the “PSCAD/EMTDC" code, it is found that these control methods can drastically reduce the reactive power induced by the four units of the “Booster PS". In addition, the voltage fluctuation of the motor-generator connected to the “Booster PS" is expected to be suppressed. This can also contribute to achieve stable control of the JT-60SA magnet power supplies.

  19. [SciELO Public Health: the performance of Cadernos de Saúde Pública and Revista de Saúde Pública].

    PubMed

    Barata, Rita Barradas

    2007-12-01

    The aim of this paper was to analyze two Brazilian scientific journals included in the SciELO Library of Public Health, using a group of bibliometric indicators and scrutinizing the articles most viewed. Cadernos de Saúde Pública was accessed 3,743.59 times per month, with an average of 30.31 citations per article. The 50 articles most viewed (6.72 to 524.5 views) were mostly published in Portuguese (92%). 42% were theoretical essays, 20% surveys, and 16% descriptive studies. 42% used argumentative techniques, 34% quantitative techniques, 18% qualitative techniques, and 6% mathematical modeling. The most common themes were: health and work (50%), epidemiology (22%), and environmental health (8%). Revista de Saúde Pública was accessed 1,590.97 times per month, with an average of 26.27 citations per article. The 50 articles most viewed (7.33 and 56.50 views) were all published in Portuguese: 46% were surveys, 14% databases analysis, and 12% systematic reviews. Quantitative techniques were adopted in 66% of such articles, while mathematical modeling was the same as observed in Cadernos de Saúde Pública, as were qualitative techniques. The most common themes were health services organization (22%), nutrition (22%), health and work (18%), epidemiology (12%), and environmental health (12%). PMID:18157346

  20. Qualification of High Specific Energy Li-Ion Cell VES180SA

    NASA Astrophysics Data System (ADS)

    Defer, M.; Du Peyrat, D.; Prevot, D.; Borthomieu, Y.

    2008-09-01

    In order to improve the competitiveness of future platform by reducing weight and cost of the flight batteries solutions, a high specific energy VES180SA cell has been developed and qualified jointly with ESA, CNES, Astrium and Thales in the frame of Artes8 program. This new generation of cell achieves a specific energy of 155Wh/kg in the same casing as the previous generation of VES140S/SA cells given for 115Wh/kg and takes benefit of:* Use of a Ni based G5 generation electrochemistry already implemented in mass production for industrial application.* Use of return on G4 (VES140) & G5 life tests results as well as VES140S/SA flight experience * Use of SA mechanics i.e. with jelly roll attached in order to sustain more severe mechanical environmentVES180SA cell design is the same as the existing VES140SA cell design except the electrochemistry (jelly-roll) which has evolved in order to deliver more energy. The cell casing is composed by a thin al. tube closed by two al. covers that are welded on the extremities of the tube. During the whole cell lifetime, casing is stressed by combined static loads due to internal gas pressure, in cycling jelly-roll swelling and irreversible jelly roll swelling due to ageing phenomena. In addition to that, static loads are temporarily combined to dynamic loads during the launch phase. Whereas, dynamic loads applied on VES140SA and VES180SA cells are the same, maximal internal gas pressure and jelly-roll swelling are higher in VES180SA cell due to its new electrochemistry. The maximal internal pressure of a cell can be estimated by a Saft numerical model based on pressure measurement and extrapolations. For what concern the jelly roll swelling, such a model was not available at the start of the development of the VES180SA cell. The aim of the cell development phase was therefore to optimize the design by characterizing the casing material and performing adequate tests in order to define the cell worst case mechanical loads as a

  1. G-LoSA: An efficient computational tool for local structure-centric biological studies and drug design.

    PubMed

    Lee, Hui Sun; Im, Wonpil

    2016-04-01

    Molecular recognition by protein mostly occurs in a local region on the protein surface. Thus, an efficient computational method for accurate characterization of protein local structural conservation is necessary to better understand biology and drug design. We present a novel local structure alignment tool, G-LoSA. G-LoSA aligns protein local structures in a sequence order independent way and provides a GA-score, a chemical feature-based and size-independent structure similarity score. Our benchmark validation shows the robust performance of G-LoSA to the local structures of diverse sizes and characteristics, demonstrating its universal applicability to local structure-centric comparative biology studies. In particular, G-LoSA is highly effective in detecting conserved local regions on the entire surface of a given protein. In addition, the applications of G-LoSA to identifying template ligands and predicting ligand and protein binding sites illustrate its strong potential for computer-aided drug design. We hope that G-LoSA can be a useful computational method for exploring interesting biological problems through large-scale comparison of protein local structures and facilitating drug discovery research and development. G-LoSA is freely available to academic users at http://im.compbio.ku.edu/GLoSA/. PMID:26813336

  2. Genome Sequence of Klebsiella oxytoca SA2, an Endophytic Nitrogen-Fixing Bacterium Isolated from the Pioneer Grass Psammochloa villosa

    PubMed Central

    Chen, Mingyue; Lin, Li; Zhang, Yanming; Sun, Li

    2013-01-01

    Klebsiella oxytoca strain SA2 is an endophytic nitrogen-fixing bacterium isolated from the pioneer grass Psammochloa villosa, which grows in the moving sand dunes of Ordos Plateau, China. The SA2 genome sequence provides the genetic background for understanding its endophytic lifestyle and survival in association with grass in nitrogen-poor environments. PMID:23950120

  3. Genome Sequence of Klebsiella oxytoca SA2, an Endophytic Nitrogen-Fixing Bacterium Isolated from the Pioneer Grass Psammochloa villosa.

    PubMed

    Chen, Mingyue; Lin, Li; Zhang, Yanming; Sun, Li; An, Qianli

    2013-01-01

    Klebsiella oxytoca strain SA2 is an endophytic nitrogen-fixing bacterium isolated from the pioneer grass Psammochloa villosa, which grows in the moving sand dunes of Ordos Plateau, China. The SA2 genome sequence provides the genetic background for understanding its endophytic lifestyle and survival in association with grass in nitrogen-poor environments. PMID:23950120

  4. 75 FR 53609 - Airworthiness Directives; Empresa Brasileira de Aeronautica S.A. (EMBRAER) Model EMB-500 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-01

    ... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or... Directives; Empresa Brasileira de Aeronautica S.A. (EMBRAER) Model EMB-500 Airplanes AGENCY: Federal Aviation... docket. Relevant Service Information Empresa Brasileira de Aeron utica S.A. (EMBRAER) has issued...

  5. 77 FR 32437 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-01

    ... different compliance time. We are proposing this AD to prevent rupture of a gas generator (GG) turbine blade...-13-05, Amendment 39-16728 (76 FR 40222, July 8, 2011) for Turbomeca S.A. Arriel 2B and 2B1 turboshaft... AD resulted from several cases of GG turbine blade rupture occurring in service on Arriel 2...

  6. Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA)

    PubMed Central

    Wu, Guanxun; Li, Guoqiang; Sun, Haifeng; Deng, Suzhen; Shen, Yicheng; Chen, Guoqiang; Zhang, Ruihao; Meng, Chunxiao; Zhang, Xiaowen

    2015-01-01

    Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (~9.99%), 5302 (~9.45%), 5242 (~9.35%), 4407 (~7.86%) and 4195 (~7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis. PMID:26484871

  7. Phillips SA8016BW 2.5 GHz Synthesizer SEE Testing

    NASA Technical Reports Server (NTRS)

    Carts, Marty; Ladbury, Ray; Marshall, Paul W.; Mackey, Susan

    2008-01-01

    This viewgraph presentation reviews the Single Event Effects (SEE) testing of the Phillips SA8016BW 2.5 GHz Synthesizer that was chose by the GLAST Program for Frequency Generation. Included in this are diagrams of the phased-locked loop (PLL), the synthesizer, and heater.

  8. 75 FR 53857 - Airworthiness Directives; Eurocopter France Model SA330J Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-02

    ... Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477-78). Regulatory... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant... Model SA330J Helicopters AGENCY: Federal Aviation Administration, DOT. ACTION: Final rule; request...

  9. A study of the Sa-Ahm Five Element acupuncture theory.

    PubMed

    Ahn, Chang-Beohm; Jang, Kyung-Jun; Yoon, Hyun-Min; Kim, Cheol-Hong; Min, Young-Kwang; Song, Chun-Ho; Lee, Jang-Cheon

    2009-12-01

    This study is a review of the theoretical basis of Sa-Ahm Five Element acupuncture devised about 360 years ago. A total of 21 books and papers ranging from the ancient Huang Di Nei Jing to modern biomedical acupuncture for pain management were researched in relation to the Five Shu points. Gao-Wu, in the Chinese Ming Dynasty, used the Five Shu points for the first time based on the creation cycle as the tonification and sedation treatment, termed the "tonification and sedation treatment of self meridian". Since then, and for no particular reason, this method has been rarely used until Sa-Ahm's new doctrine, which includes the concept of the destruction cycle, was asserted. Sa-Ahm Five Element acupuncture is a method in which the Five Shu points are used from the viewpoints of the simultaneous tonification and sedation methods that are based on the promotion and control cycles. Although it is currently the most utilized method in accordance with the practitioner's points, this method is in need of designated guidelines by which Sa-Ahm acupuncture can be practiced effectively. PMID:20633508

  10. [Study on structural variation of nucleolar organizer regions (NOR) and the satellite association (SA)].

    PubMed

    Wang, G H

    1989-01-01

    To further illustrate the biological significance of variant NOR (including double NOR--dNOR and triple NOR--tNOR) and satellite association (SA) and the relationship to meiotic non-disjunction (NDJ), this study was carried out in the normal population and the parents of patients with Down Syndrome (DS). Of 800 individuals, 4 dNOR carriers, two of them from the control and two from the non-informative experiments were detected. SA data for 3 out of the 4 dNOR carriers showed no statistical significant increase in SA when dNOR was present in the cell even in case 4 where two dNOR were found in one cell. However, ease 3 demonstrated significant decrease in SA when dNOR was present, although it showed that the overall incidence of dNOR was higher in this carrier than that in the other three. It is concluded that dNOR can be detected on any acrocentric chromosome and not be related to NDJ of chromosome, which is a heritable variant that does not affect phenotype and that there are two kinds of dNOR variants, one being from the translocation of the short arm of acrocentric chromosome, the other from non-translocation. PMID:2534280

  11. 78 FR 275 - Airworthiness Directives; REIMS Aviation S.A. Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-03

    ... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034...; ] DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; REIMS Aviation S.A. Airplanes AGENCY: Federal Aviation Administration (FAA), Department...

  12. SEU response of design- and resistor-hardened D-latches in the SA3300 microprocessor

    SciTech Connect

    Sexton, F.W.; Corbett, W.T.; Axness, C.L.; Wunsch, T.F.; Hughes, K.L.; Treece, R.K.; Hass, K.J.; Hash, G.L.; Shaneyfelt, M.R.

    1991-01-01

    The effectiveness of hardening the SA3300 against SEU using design and design-plus-resistor approaches is presented. The full performance and SEU tolerance requirements are met using R{sub fb} {le} 160 k{Omega}. Lumped-parameter circuit simulations are used to analyze results. 10 refs., 5 figs.

  13. The effect of net charge on the solubility, activity, and stability of ribonuclease Sa

    PubMed Central

    Shaw, Kevin L.; Grimsley, Gerald R.; Yakovlev, Gennady I.; Makarov, Alexander A.; Pace, C. Nick

    2001-01-01

    The net charge and isoelectric pH (pI) of a protein depend on the content of ionizable groups and their pK values. Ribonuclease Sa (RNase Sa) is an acidic protein with a pI = 3.5 that contains no Lys residues. By replacing Asp and Glu residues on the surface of RNase Sa with Lys residues, we have created a 3K variant (D1K, D17K, E41K) with a pI = 6.4 and a 5K variant (3K + D25K, E74K) with a pI = 10.2. We show that pI values estimated using pK values based on model compound data can be in error by >1 pH unit, and suggest how the estimation can be improved. For RNase Sa and the 3K and 5K variants, the solubility, activity, and stability have been measured as a function of pH. We find that the pH of minimum solubility varies with the pI of the protein, but that the pH of maximum activity and the pH of maximum stability do not. PMID:11369859

  14. 77 FR 65581 - Verizon Business Networks Services, Inc., Senior Analyst, Service Program Delivery (SA-SPD...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-29

    ... Employment and Training Administration Verizon Business Networks Services, Inc., Senior Analyst, Service Program Delivery (SA-SPD), Including Workers Whose Wages Were Paid Under MCI Communication Services, Inc..., 2012, applicable to workers and former workers of Verizon Business Network Services, Inc.,...

  15. Weasel works SA-150: Design study of a 100 to 150 passenger transport aircraft

    NASA Technical Reports Server (NTRS)

    Alkema, Kevin; Comeaux, Michael; Gilbert, Timothy; Para, Victor; Toepfer, George

    1993-01-01

    As the year 2000 rapidly approaches, the airlines are faced with an extremely competitive and environmentally restrictive marketplace. In order to survive, commercial air carriers will need to find new ways to lower their direct operating costs, increase load factors and comply with tightening federal and international constraints. The SA-150 has been designed to meet these demands by focusing on the areas of aerodynamic efficiency, an improved level of passenger comfort, and a limited application of advanced technology. The SA-150 has been optimized for a 500 nmi. mission to help the airlines meet the challenges of the short haul, quick turnaround flight. With a maximum capacity of 124 passengers, and full baggage, the SA-150 is also capable of covering a range of 1500 nmi. This additional range capability will provide the airlines with flexibility when scheduling their routes. The aircraft features a 'V' tail, fly-by-wire system and is powered by two turbofans mounted under a twelve aspect ratio wing. The SA-150 will have an initial production run of 800 units and have a purchase price of $37.7 million in 1993 dollars.

  16. 78 FR 48824 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-12

    ... Register published on April 11, 2000 (65 FR 19477-78). Discussion The European Aviation Safety Agency (EASA... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... was prompted by a ``chip illumination event'' in flight on a Turbomeca S.A. Arriel 1 engine....

  17. saRNA-guided Ago2 targets the RITA complex to promoters to stimulate transcription

    PubMed Central

    Portnoy, Victoria; Lin, Szu Hua Sharon; Li, Kathy H; Burlingame, Alma; Hu, Zheng-Hui; Li, Hao; Li, Long-Cheng

    2016-01-01

    Small activating RNAs (saRNAs) targeting specific promoter regions are able to stimulate gene expression at the transcriptional level, a phenomenon known as RNA activation (RNAa). It is known that RNAa depends on Ago2 and is associated with epigenetic changes at the target promoters. However, the precise molecular mechanism of RNAa remains elusive. Using human CDKN1A (p21) as a model gene, we characterized the molecular nature of RNAa. We show that saRNAs guide Ago2 to and associate with target promoters. saRNA-loaded Ago2 facilitates the assembly of an RNA-induced transcriptional activation (RITA) complex, which, in addition to saRNA-Ago2 complex, includes RHA and CTR9, the latter being a component of the PAF1 complex. RITA interacts with RNA polymerase II to stimulate transcription initiation and productive elongation, accompanied by monoubiquitination of histone 2B. Our results establish the existence of a cellular RNA-guided genome-targeting and transcriptional activation mechanism and provide important new mechanistic insights into the RNAa process. PMID:26902284

  18. Resisting Colonial Education: Zitkala-Sa and Native Feminist Archival Refusal

    ERIC Educational Resources Information Center

    Terrance, Laura L.

    2011-01-01

    This paper examines resistance through a Native Feminist lens, employing the boarding school memoirs of Zitkala-Sa. Within a "story" of appropriation in methodology, it considers protest and parody, and presents archival refusal as modes of resistance to colonial education. (Contains 1 figure.)

  19. 76 FR 33298 - Grifols, S.A. and Talecris Biotherapeutics Holdings Corp.; Analysis of Agreement Containing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... Grifols, S.A. and Talecris Biotherapeutics Holdings Corp.; Analysis of Agreement Containing Consent Orders... deceptive acts or practices or unfair methods of competition. The attached Analysis to Aid Public Comment... Commission, has been placed on the public record for a period of thirty (30) days. The following Analysis...

  20. 77 FR 43552 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-25

    ... 2009-03-04, Amendment 39-15805 (74 FR 7796, February 20, 2009), for all Turbomeca S.A. Arriel 1E2, 1S.... Proposed AD Requirements This proposed AD would retain all of the requirements of AD 2009- 03-04, (74 FR... a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February...

  1. Saturn 1B launch vehicle flight evaluation report-SA-207: Skylab-3

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The performance evaluation of the SA-207 launch vehicle for the Skylab 3 launch is presented. Flight problems are identified, the causes are determined, and recommendations are made for appropriate corrective action. Summaries of launch operations and spacecraft performance are included. The significant events, failures, and anomalies are tabulated.

  2. Saturn 1B launch vehicle flight evaluation report SA-208 (Skylab-4)

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The results of the Saturn 1B, SA-208 launch vehicle flight evaluation are presented. The performance of the launch vehicle is analyzed with special emphasis given to identifying the causes of flight problems. Recommendations for corrective action and summaries of launch operations and spacecraft performance are included.

  3. Saturn 1B launch vehicle flight evaluation report, SA-206, Skylab-2

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The performance evaluation of the SA-206 launch vehicle for the Skylab 2 launch is presented. Flight problems are identified, the causes are determined, and recommendations are made for appropriate corrective action. Summaries of launch operations and spacecraft performance are included. The significant events, failures, and anomalies are tabulated.

  4. Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA).

    PubMed

    Gao, Zhengquan; Li, Yan; Wu, Guanxun; Li, Guoqiang; Sun, Haifeng; Deng, Suzhen; Shen, Yicheng; Chen, Guoqiang; Zhang, Ruihao; Meng, Chunxiao; Zhang, Xiaowen

    2015-01-01

    Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (~9.99%), 5302 (~9.45%), 5242 (~9.35%), 4407 (~7.86%) and 4195 (~7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis. PMID:26484871

  5. 78 FR 66258 - Airworthiness Directives; Empresa Brasileira de Aeronautica S.A. (EMBRAER) Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); 3. Will not affect intrastate aviation... de Aeronautica S.A. (EMBRAER) Airplanes AGENCY: Federal Aviation Administration (FAA), Department of..., and -135LR airplanes; and Model EMB-145, -145ER, -145MR, -145LR, -145XR, -145MP, and -145EP...

  6. 78 FR 11609 - Special Conditions: Embraer S.A., Model EMB-550 Airplane; Landing Pitchover Condition

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ...This action proposes special conditions for the Embraer S.A. Model EMB-550 airplane. This airplane will have a novel or unusual design feature(s) associated with landing loads due to the automatic braking system. The applicable airworthiness regulations do not contain adequate or appropriate safety standards for this design feature. These proposed special conditions contain the additional......

  7. SA-based concrete seismic stress monitoring: a case study for normal strength concrete

    NASA Astrophysics Data System (ADS)

    Hou, S.; Zhang, H. B.; Ou, J. P.

    2016-09-01

    The stress history of concrete structures that have survived an earthquake can serve as a critical index to evaluate the health of the structure. There are currently few reliable monitoring methods to assess concrete stress after a seismic event. Piezoelectric-based smart aggregate (SA) provides an innovative experimental approach to monitor stress on concrete. The principle of SA-based concrete seismic stress monitoring is based on the assumption that concrete stress can be reliably predicted by the average output voltages of limited SAs with an acceptable margin of error. In this study, the meso-scale randomness of concrete was evaluated throughout the overall stress range of concrete and the influence of different load paths was considered. Four cylindrical specimens of normal strength concrete were embedded with a total of 24 SAs. The SA output sensitivity curve in the paths of loading–unloading with different amplitudes and monotonic loading up to failure was obtained. Monitoring errors were analyzed during pre- and post-peak stages from the experimental results. This research suggests that SA-based concrete seismic stress monitoring for normal strength concrete is reliable.

  8. "They Might as Well Be Black": The Racialization of Sa'moan High School Students

    ERIC Educational Resources Information Center

    Vaught, Sabina Elena

    2012-01-01

    This article explores the processes of racialization imposed on Sa'moan youth through policy and practice in one urban, US school district and at one high school in particular. Specifically, I use the methodological practices of defamiliarization and counter-storytelling to examine the contradictory practices of racialization and the…

  9. 78 FR 34605 - Airworthiness Directives; CFM International S.A. Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-10

    ... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will...; International fax: 1-513-552-3329; email: geae.aoc@ge.com ; or CFM International SA, Customer Support Center, International phone: 33 1 64 14 88 66; fax: 33 1 64 79 85 55; email: snecma.csc@snecma.fr . (3) You may...

  10. 78 FR 6195 - Special Conditions: Embraer S.A., Model EMB-550 Airplanes; Flight Envelope Protection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... Embraer S.A. Model EMB-550 airplanes was published in the Federal Register on November 9, 2012, (77 FR... Protection: Performance Credit for Automatic Takeoff Thrust Control System (ATTCS) During Go-Around AGENCY... design feature associated with the use of an Automatic Takeoff Thrust Control System (ATTCS) during...

  11. Resisting the Script of Indian Education: Zitkala Sa and the Carlisle Indian School.

    ERIC Educational Resources Information Center

    Enoch, Jessica

    2002-01-01

    Offers a "definition" of Zitkala Sa as an Indian teacher who, at the turn of the 20th century, challenged and countered educational norms that silenced Indian voices and erased Indian culture. Examines her autobiographical essays, "Impressions of an Indian Childhood,""The School Days of an Indian Girl," and "An Indian Teacher among Indians," in…

  12. Small Scale Trace Contaminant Testing of SA9T at Ambient and Reduced Pressure Conditions

    NASA Technical Reports Server (NTRS)

    Broerman, Craig; Sweterlitsch, Jeffrey

    2011-01-01

    A principle concern for air revitalization technology in a closed loop system is the capability to control carbon dioxide (CO2) and humidity (H2O). An amine based sorbent technology, SA9T, has long been evaluated for use in this application and several programs are evaluating it for use in both a cabin as well as space suit applications. While the CO2 and H2O performance of the sorbent has been tested extensively, the question of how trace contaminants impact performance requires further evaluation. This paper presents experimental results of small scale SA9T testing that was performed over a variety of test conditions and with a variety of trace contaminants. Testing evaluated the ability of SA9T media to sufficiently remove CO2 and H2O after exposure to a fully saturated trace contaminant at ambient conditions. Testing also evaluated the impact of CO2 and H2O removal performance at suit loop pressures during cyclic operation with a constant inlet contaminant load. In addition, testing evaluated the performance of SA9T at ambient conditions in a continuous 30-day test with a mixed trace contaminant stream.

  13. Bench-Scale Trace Contaminant Testing of SA9T at Ambient and Reduced Pressure Conditions

    NASA Technical Reports Server (NTRS)

    Broerman, Craig; Sweterlitsch, Jeff

    2011-01-01

    A principal concern for air revitalization technology in a closed loop system is the capability to control carbon dioxide (CO2) and humidity (H2O). An amine based sorbent technology, SA9T, has been evaluated for use in this application and several programs are evaluating it for use in both cabin and space suit applications. While the CO2 and H2O performance of the sorbent has been tested extensively, the question of how trace contaminants impact performance requires further evaluation. This paper presents experimental results of bench-scale SA9T testing that was performed under a variety of test conditions and with several different trace contaminants. Tests were conducted to determine if the capacity of the SA9T media to sufficiently remove CO2 and H2O is compromised after exposure to a fully saturated trace contaminant at ambient conditions. Tests also were conducted to evaluate the performance of SA9T at ambient conditions in a continuous 30-day test with a mixed trace contaminant stream. In addition, testing also evaluated the impact of CO2 and H2O removal performance at suit loop pressures (29.6 KPa/4.3 psia) during cyclic operation with a constant inlet contaminant load.

  14. 78 FR 34284 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-07

    ... 2013-01-07, Amendment 39-17321 (78 FR 6725, January 31, 2013), for all Turbomeca S.A. Arriel 2D... helicopter. Actions Since Existing AD Was Issued Since we issued AD 2013-01-07 (78 FR 6725, January 31, 2013... would maintain the existing AD requirements of AD 2013-01-07 (78 FR 6725, January 31, 2013), and...

  15. Binding Sites Analyser (BiSA): Software for Genomic Binding Sites Archiving and Overlap Analysis

    PubMed Central

    Khushi, Matloob; Liddle, Christopher; Clarke, Christine L.; Graham, J. Dinny

    2014-01-01

    Genome-wide mapping of transcription factor binding and histone modification reveals complex patterns of interactions. Identifying overlaps in binding patterns by different factors is a major objective of genomic studies, but existing methods to archive large numbers of datasets in a personalised database lack sophistication and utility. Therefore we have developed transcription factor DNA binding site analyser software (BiSA), for archiving of binding regions and easy identification of overlap with or proximity to other regions of interest. Analysis results can be restricted by chromosome or base pair overlap between regions or maximum distance between binding peaks. BiSA is capable of reporting overlapping regions that share common base pairs; regions that are nearby; regions that are not overlapping; and average region sizes. BiSA can identify genes located near binding regions of interest, genomic features near a gene or locus of interest and statistical significance of overlapping regions can also be reported. Overlapping results can be visualized as Venn diagrams. A major strength of BiSA is that it is supported by a comprehensive database of publicly available transcription factor binding sites and histone modifications, which can be directly compared to user data. The documentation and source code are available on http://bisa.sourceforge.net PMID:24533055

  16. Discriminative Stimulus Properties of the Endocannabinoid Catabolic Enzyme Inhibitor SA-57 in Mice.

    PubMed

    Owens, Robert A; Ignatowska-Jankowska, Bogna; Mustafa, Mohammed; Beardsley, Patrick M; Wiley, Jenny L; Jali, Abdulmajeed; Selley, Dana E; Niphakis, Micah J; Cravatt, Benjamin F; Lichtman, Aron H

    2016-08-01

    Whereas the inhibition of fatty acid amide hydrolase (FAAH) or monoacylglycerol lipase (MAGL), the respective major hydrolytic enzymes of N-arachidonoyl ethanolamine (AEA) and 2-arachidonoylglycerol (2-AG), elicits no or partial substitution for Δ(9)-tetrahydrocannabinol (THC) in drug-discrimination procedures, combined inhibition of both enzymes fully substitutes for THC, as well as produces a constellation of cannabimimetic effects. The present study tested whether C57BL/6J mice would learn to discriminate the dual FAAH-MAGL inhibitor SA-57 (4-[2-(4-chlorophenyl)ethyl]-1-piperidinecarboxylic acid 2-(methylamino)-2-oxoethyl ester) from vehicle in the drug-discrimination paradigm. In initial experiments, 10 mg/kg SA-57 fully substituted for CP55,940 ((-)-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans-4-(3-hydroxypropyl)cyclohexanol), a high-efficacy CB1 receptor agonist in C57BL/6J mice and for AEA in FAAH (-/-) mice. Most (i.e., 23 of 24) subjects achieved criteria for discriminating SA-57 (10 mg/kg) from vehicle within 40 sessions, with full generalization occurring 1 to 2 hours postinjection. CP55,940, the dual FAAH-MAGL inhibitor JZL195 (4-​nitrophenyl 4-​(3-​phenoxybenzyl)piperazine-​1-​carboxylate), and the MAGL inhibitors MJN110 (2,5-dioxopyrrolidin-1-yl 4-(bis(4-chlorophenyl)methyl)piperazine-1-carboxylate) and JZL184 (4-[Bis(1,3-benzodioxol-5-yl)hydroxymethyl]-1-piperidinecarboxylic acid 4-nitrophenyl ester) fully substituted for SA-57. Although the FAAH inhibitors PF-3845 ((N-3-pyridinyl-4-[[3-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenyl]methyl]-1-piperidinecarboxamide) and URB597 (cyclohexylcarbamic acid 3'-(aminocarbonyl)-[1,1'-biphenyl]-3-yl ester) did not substitute for SA-57, PF-3845 produced a 2-fold leftward shift in the MJN110 substitution dose-response curve. In addition, the CB1 receptor antagonist rimonabant blocked the generalization of SA-57, as well as substitution of CP55,940, JZL195, MJN110, and JZL184. These findings

  17. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction.

    PubMed

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de Los Santos, Berta; Arroyo, Francisco T; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  18. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

    PubMed Central

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de los Santos, Berta; Arroyo, Francisco T.; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L.

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  19. Total synthesis and evaluation of iso-duocarmycin SA and iso-yatakemycin.

    PubMed

    MacMillan, Karen S; Nguyen, Trinh; Nguyen, Trihn; Hwang, Inkyu; Boger, Dale L

    2009-01-28

    The total synthesis and evaluation of iso-duocarmycin SA (5) and iso-yatakemycin (6), representing key analogues of the corresponding natural products incorporating an isomeric alkylation subunit, are detailed. This pyrrole isomer of the natural alkylation subunit displayed an enhanced reaction regioselectivity and a 2-fold diminished stability. Although still exceptionally potent, the iso-duocarmycin SA derivatives and natural product analogues exhibited a corresponding approximate 3-5-fold reduction in cytotoxic activity [L1210 IC(50) for (+)-iso-duocarmycin SA = 50 pM and for (+)-iso-yatakemycin = 15 pM] consistent with their placement on a parabolic relationship correlating activity with reactivity. The DNA alkylation selectivity of the resulting key natural product analogues was unaltered by the structure modification in spite of the minor-groove presentation of a potential H-bond donor. Additionally, a unique ortho-spirocyclization with such derivatives was explored via the preparation, characterization, and evaluation of 34 that is incapable of the more conventional para-spirocyclization. Although 34 proved sufficiently stable for isolation and characterization, it displayed little stability in protic solvents (t(1/2) = 0.19 h at pH 3, t(1/2) = 0.20 h at pH 7), a pH-independent (H(+) independent) solvolysis rate profile at pH 3/4-7, and a much reduced cytotoxic potency, but a DNA alkylation selectivity and efficiency comparable to those of duocarmycin SA and iso-duocarmycin SA. The implications of these observations on the source of the DNA alkylation selectivity and catalysis for this class of natural products are discussed. PMID:19154178

  20. Establishment and gene analysis of a cisplatin-resistant cell line, Sa-3R, derived from oral squamous cell carcinoma.

    PubMed

    Nakatani, Ken; Nakamura, Megumi; Uzawa, Katsuhiro; Wada, Takeshi; Seki, Naohiko; Tanzawa, Hideki; Fujita, Shigeyuki

    2005-04-01

    Cisplatin (CDDP) is widely used for chemotherapy of many malignancies, especially of oral squamous cell carcinoma (SCC). However, because the mechanism of resistance to CDDP is unclear, we established a CDDP-resistant cell line, Sa-3R, from a CDDP-sensitive cell line, Sa-3, which was derived from moderately differentiated SCC of the lower gingiva. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide assay indicated that Sa-3R has 7.5-fold greater resistance to CDDP than Sa-3. Comparing gene expression levels in the cell lines using an in-house cDNA microarray, which represented 2,201 oral disease origin genes, many differentially expressed genes were identified. The ATP-binding cassette transporter genes (MDR-1, MRP-1, and MRP-2), and FANCONI, GRP58, FLJ12089, and SPINT-2 were up-regulated, whereas FOSL1, MRPS27, and PGK-1 were down-regulated. These results were confirmed by semiquantitative reverse transcriptase-polymerase chain reaction. The Sa-3/Sa-3R cell lines could be useful to identify the candidates responsible for the mechanism of CDDP-resistance and the up- or down-regulated genes identified by the gene expression profiles in the Sa-3R cell line may be, in part, associated with the mechanism. PMID:15756446

  1. Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples.

    PubMed

    Alibayov, Babek; Zdenkova, Kamila; Sykorova, Hana; Demnerova, Katerina

    2014-12-01

    Staphylococcus aureus produces a wide variety of superantigenic activity Staphylococcal enterotoxins (SE) and they are a major cause of food poisoning. These superantigens are associated with mobile genetic elements such as plasmids, prophages and S. aureus pathogenicity islands (SaPI). The presence of well-known eight SaPI integrase and 13 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sel, sek, seq, and tst) in 93 S. aureus strains were investigated. All S. aureus isolates were characterized by pulsed-field gel electrophoresis (PFGE), and the genes were detected using five sets of multiplex PCR (mPCR). The most predominant toxin genes were sea (19%), seb (15%), sec (54%), sell (48%), selk (46%), selq (52%), seg (22%), and sei (19%). Analysis showed that many S. aureus isolates harbored multiple toxin genes. An mPCR-based assay was developed for the determination of all SaPI and their superantigen gene combinations. Twenty three isolates revealed the gene combination sec, sell and tst, typical of the SaPIbov1 and SaPIn1/m1 pathogenicity islands. Twelve isolates revealed the selk and selq gene combination consistent with SaPI3. Eight isolates exhibited the sec and sell genes without the tst gene typical of SaPImw2. We established a correlation between superantigenic toxin genotypes in S. aureus in terms of combinations of toxin gene-encoding SaPI. These results provide a rapid method for determining superantigenic toxin genotypes in S. aureus strains. A total of 24 PFGE patterns were generated. To our knowledge, this is a first study analyzing the correlation of all known SaPI and their enterotoxins in S. aureus using mPCR. PMID:25447888

  2. Environmental and Mechanical Stability of Environmental Barrier Coated SA Tyrannohex SiC Composites Under Simulated Turbine Engine Environments

    NASA Technical Reports Server (NTRS)

    Zhu, Dongming; Halbig, Michael Charles; Sing, Mrityunjay

    2014-01-01

    The environmental stability and thermal gradient cyclic durability performance of SA Tyrannohex composites were investigated for turbine engine component applications. The work has been focused on investigating the combustion rig recession, cyclic thermal stress resistance and thermomechanical low cycle fatigue of uncoated and environmental barrier coated Tyrannohex SiC SA composites in simulated turbine engine combustion water vapor, thermal gradients, and mechanical loading conditions. Flexural strength degradations have been evaluated, and the upper limits of operating temperature conditions for the SA composite material systems are discussed based on the experimental results.

  3. [Output and citation in public health: a perspective based on the journals Cadernos de Saúde Pública and Revista de Saúde Pública].

    PubMed

    Carvalho, Leandro; Coimbra, Carlos E A; Souza-Santos, Reinaldo; Santos, Ricardo Ventura

    2007-12-01

    This is a comparative bibliometric study of the two main scientific journals in the Public Health field in Brazil: Cadernos de Saúde Pública (CSP) and Revista de Saúde Pública (RSP). Twenty-four issues of each periodical were analyzed, published from 1996 to 2003, comprising a sample of 819 articles (496 from CSP and 323 from RSP). The following elements in the articles were identified and analyzed: thematic area of the article and citation pattern of publications (articles in periodicals, books and/or book chapters, theses/dissertations, Internet documents, "gray" literature, and other types of publications). The resulting data showed that the majority of the articles represent the sub-area of "epidemiology". The citation pattern in the journals showed articles in periodicals in first place, followed by citations of books and/or book chapters. Papers in the sub-area "social sciences in health" published in CSP are exceptions, since books and chapters are the most frequently cited. The authors discuss the implications of the findings for the characterization and evaluation of scientific output in Public Health in Brazil. PMID:18157345

  4. Leonardo da Vinci and the Downburst.

    NASA Astrophysics Data System (ADS)

    Gedzelman, Stanley David

    1990-05-01

    Evidence from the drawings, experiments, and writings of Leonardo da Vinci are presented to demonstrate that da Vinci recognized and, possibly, discovered the downburst and understood its associated airflow. Other early references to vortex flows resembling downbursts are mentioned.

  5. An evaluation of the spring finger solder joints on SA1358-10 and SA2052-4 connector assemblies (MC3617,W87).

    SciTech Connect

    Kilgo, Alice C.; Vianco, Paul Thomas; Hlava, Paul Frank; Zender, Gary L.

    2006-08-01

    The SA1358-10 and SA2052-4 circular JT Type plug connectors are used on a number of nuclear weapons and Joint Test Assembly (JTA) systems. Prototype units were evaluated for the following specific defects associated with the 95Sn-5Sb (Sn-Sb, wt.%) solder joint used to attach the beryllium-copper (BeCu) spring fingers to the aluminum (Al) connector shell: (1) extended cracking within the fillet; (2) remelting of the solder joint during the follow-on, soldering step that attached the EMR adapter ring to the connector shell (and/or soldering the EMR shell to the adapter ring) that used the lower melting temperature 63Sn-37Pb (Sn-Pb) alloy; and (3) spalling of the Cd (Cr) layer overplating layer from the fillet surface. Several pedigrees of connectors were evaluated, which represented older fielded units as well as those assemblies that were recently constructed at Kansas City Plant. The solder joints were evaluated that were in place on connectors made with the current soldering process as well as an alternative induction soldering process for attaching the EMR adapter ring to the shell. Very similar observations were made, which crossed the different pedigrees of parts and processes. The extent of cracking in the top side fillets varied between the different connector samples and likely the EMR adapter ring to the shell. Very similar observations were made, which crossed the different pedigrees of parts and processes. The extent of cracking in the top side fillets varied between the different connector samples and likely reflected the different extents to which the connector was mated to its counterpart assembly. In all cases, the spring finger solder joints on the SA1358-10 connectors were remelted as a result of the subsequent EMR adapter ring attachment process. Spalling of the Cd (Cr) overplating layer was also observed for these connectors, which was a consequence of the remelting activity. On the other hand, the SA2052-4 connector did not exhibit evidence of

  6. A study of star formation in the disks of Sa galaxies

    SciTech Connect

    Caldwell, N.; Kennicutt, R.; Phillips, A.C.; Schommer, R.A. Steward Observatory, Tucson, AZ Washington Univ., Seattle Rutgers Univ., Piscataway, NJ )

    1991-04-01

    This paper compares the luminosity functions of the H II regions in several Sa galaxies with those of later-type galaxies. Broad UV measurements confirm expectations that the knots associated with the regions are very blue; the converse is also true. The H II region luminosity functions are very steep. The total H-alpha luminosities for the galaxies are computed and used to derive the current star-formation rates. It is found that, in contrast to the late-type galaxies, the current star-formation rates in Sa disks are less than one-tenth of the average rate over the last 15 Gyr. The formal depletion times of gas through star formation are longer than a Hubble time. If the star formation in late-type galaxies takes on the character of that currently seen in the Sas, star formation in such galaxies could continue for much longer than the usual estimate of 5 Gyr. 35 refs.

  7. Permanent draft genome sequence of the gliding predator Saprospira grandis strain Sa g1 (= HR1)

    SciTech Connect

    Mavromatis, K; Chertkov, Olga; Lapidus, Alla L.; Nolan, Matt; Lucas, Susan; Tice, Hope; Glavina Del Rio, Tijana; Cheng, Jan-Fang; Han, Cliff; Tapia, Roxanne; Bruce, David; Goodwin, Lynne A.; Pitluck, Sam; Huntemann, Marcel; Liolios, Konstantinos; Pagani, Ioanna; Ivanova, N; Mikhailova, Natalia; Pati, Amrita; Chen, Amy; Palaniappan, Krishna; Land, Miriam L; Brambilla, Evelyne-Marie; Rohde, Manfred; Spring, Stefan; Goker, Markus; Detter, J. Chris; Bristow, James; Eisen, Jonathan; Markowitz, Victor; Hugenholtz, Philip; Kyrpides, Nikos C; Klenk, Hans-Peter; Woyke, Tanja

    2012-01-01

    Saprospira grandis Gross et al. 1911 is a member of the Saprospiraceae, a family in the class 'Sphingobacteria' that remains poorly characterized at the genomic level. The species is known for preying on other marine bacteria via 'ixotrophy'. S. grandis strain Sa g1 was isolated from decaying crab carapace in France and was selected for genome sequencing because of its isolated location in the tree of life. Only one type strain genome has been published so far from the Saprospiraceae, while the sequence of strain Sa g1 represents the second genome to be published from a non-type strain of S. grandis. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,495,250 bp long Improved-High-Quality draft of the genome with its 3,536 protein-coding and 62 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  8. STS-48 ESC closeup of extended UARS solar array (SA) during pre-deploy check

    NASA Technical Reports Server (NTRS)

    1991-01-01

    The leading edge of the Upper Atmosphere Research Satellite (UARS) solar array (SA), fully deployed, is recorded by the electronic still camera (ESC). UARS, grappled by the remote manipulator system (RMS) end effector (out of frame), is undergoing STS-48 pre-deployment checkout above the payload bay (PLB) of the earth-orbiting Discovery, Orbiter Vehicle (OV) 103. A hinge and the pyrotechnic bolts that enable the SA to deploy can be seen in this crisp image. This view demonstrates the capabilities of the ESC to provide high resolution views of hardware for review by ground controllers. This ESC image was documented as part of Development Test Objective (DTO) 648, Electronic Still Photography. The digital image was stored on a removable hard disk or small optical disk, and could be converted to a format suitable for downlink transmission.

  9. A brief history of cadernos de Saúde Pública/reports in Public Health.

    PubMed

    Carvalho, Marilia Sá; Coeli, Cláudia Medina; Travassos, Claudia

    2015-07-01

    This article was written as part of the 20th-year commemorative issue of Ciência e Saúde Coletiva (C&SC). We take advantage of this opportunity to revisit the successful history of Cadernos de Saúde Pública (CSP)/Reports in Public Health in its 30 years of publication, progressing from a quarterly periodical with limited circulation to a monthly journal indexed in the main scientific databases. We also summarize some themes from our recent editorials, including the internationalization of scientific journals, the demand for relevant and original articles, plagiarism, and peer review. We also discuss the article submission management system developed by CSP and conclude with ideas on cooperation between journals in the public health field. PMID:26132239

  10. [Programa de saúde: a case of book censorship during the Brazilian military dictatorship].

    PubMed

    Reimão, Sandra

    2013-11-30

    Some documents produced by the government during the Brazilian military dictatorship have been made available for reference, including censors' reports on television and radio programs, plays, films, songs, advertisements and printed matter. Of the 500 or so reports on books, there is one that vetoes Programa de saúde: projetos e temas de higiene e saúde, an educational work that proposed actions to help students understand their sanitary conditions and acquire knowledge about health and health-promoting habits in this area. The argument for vetoing it was that the information could fall into the hands of poorly trained teachers, who could distort its content, causing serious damage to Brazilian youth. In this report we see evidence of the obscurantist, elitist posture adopted by the Department of Censorship of Public Entertainment, which aimed to restrict the circulation of information and curb debate. PMID:24346208

  11. Green synthesis and surface properties of Fe3O4@SA core-shell nanocomposites

    NASA Astrophysics Data System (ADS)

    Cao, Huimin; Li, Juchuan; Shen, Yuhua; Li, Shikuo; Huang, Fangzhi; Xie, Anjian

    2014-05-01

    In this paper, a one-step, economic and green approach was explored to prepare Fe3O4 nanoparticles by using L-cysteine as reducer and disperser without any inert gas protection. The Fe3O4 nanoparticles were then modified with stearic acid (SA) to form Fe3O4@SA core-shell nanocomposites. The experiment results indicate that the core-shell nanocomposites prepared could form monolayer on the water surface or films by means of Langmuir-Blodgett (LB) technology due to their hydrophobic and lipophilic properties. Also the composites exhibit paramagnetism, which make product dispersed stably in the oil medium to form magnetic fluid. Moreover, they are developed as sorbents to remove oil from water surface.

  12. RiSA: A Science Festival for the Bilingual and Bicultural Rio Grande Valley

    NASA Astrophysics Data System (ADS)

    Key, Joey Shapiro; Torres, Cristina; Stone, Robert

    2014-03-01

    The Rio Grande Science and Arts (RiSA) Festival organized by the Center for Gravitational Wave Astronomy (CGWA) at the University of Texas at Brownsville (UTB) will use a wide variety of artforms to bring physics and science topics to the bilingual and bicultural population of the Rio Grande Valley of South Texas. The science and art faculty at UTB will partner with art and education professionals to create an annual community event celebrating science though art. Music, dance, poetry, and visual arts will headline the festival activities. Festival events and products will be produced in both English and Spanish to attract and inform the bilingual local community. The RiSA Festival is supported by the Science Festival Alliance and the Sloan Foundation. Supported by the Science Festival Alliance and the Sloan Foundation.

  13. Progress of ITER and JT-60SA Magnet Development in Japan

    NASA Astrophysics Data System (ADS)

    Koizumi, N.; Nunoya, Y.; Yoshida, K.; Barabaschi, P.

    Japan Atomic Energy Agency (JAEA), as the Japan Domestic Agency (JADA), has the responsibility to procure 9 ITER toroidal field (TF) coils, 19 TF coil (TFC) structures, 25% of the TF conductors and 100% of the central solenoid (CS) conductors in ITER and, in addition, CS and equilibrium field (EF) coils including their conductors in JT-60SA, which is being developed as a satellite facility for ITER. In ITER, more than 90% of TF conductor fabrication was completed and finalization of the manufacturing procedure of TF coils is in progress through full-scale trials, such as trial fabrication of dummy double pancakes (DPs). In JT-60SA, fabrication of the EF4 coil was completed and the EF5 and EF6 coils are being assembled.

  14. Plasma regimes and research goals of JT-60SA towards ITER and DEMO

    NASA Astrophysics Data System (ADS)

    Kamada, Y.; Barabaschi, P.; Ishida, S.; Ide, S.; Lackner, K.; Fujita, T.; Bolzonella, T.; Suzuki, T.; Matsunaga, G.; Yoshida, M.; Shinohara, K.; Urano, H.; Nakano, T.; Sakurai, S.; Kawashima, H.; JT-60SA Team

    2011-07-01

    The JT-60SA device has been designed as a highly shaped large superconducting tokamak with a variety of plasma actuators (heating, current drive, momentum input, stability control coils, resonant magnetic perturbation coils, W-shaped divertor, fuelling, pumping, etc) in order to satisfy the central research needs for ITER and DEMO. In the ITER- and DEMO-relevant plasma parameter regimes and with DEMO-equivalent plasma shapes, JT-60SA quantifies the operation limits, plasma responses and operational margins in terms of MHD stability, plasma transport and confinement, high-energy particle behaviour, pedestal structures, scrape-off layer and divertor characteristics. By integrating advanced studies in these research fields, the project proceeds 'simultaneous and steady-state sustainment of the key performances required for DEMO' with integrated control scenario development applicable to the highly self-regulating burning high-β high bootstrap current fraction plasmas.

  15. Spatial Pattern Detection of Tuberculosis: A Case Study of Si Sa Ket Province, Thailand

    PubMed Central

    Hassarangsee, Siriwan; Tripathi, Nitin Kumar; Souris, Marc

    2015-01-01

    This retrospective population-based study was conducted to analyze spatial patterns of tuberculosis (TB) incidence in Si Sa Ket province, Thailand. TB notification data from 2004 to 2008 collected from TB clinics throughout the province was used along with population data to reveal a descriptive epidemiology of TB incidences. Global clustering patterns of the occurrence were assessed by using global spatial autocorrelation techniques. Additionally, local spatial pattern detection was performed by using local spatial autocorrelation and spatial scan statistic methods. The findings indicated clusters of the disease occurred in the study area. More specifically, significantly high-rate clusters were mostly detected in Mueang Si Sa Ket and Khukhan districts, which are located in the northwestern part of the province, while significantly low-rate clusters were persistent in Kantharalak and Benchalak districts, which are located at the southeastern area. PMID:26694437

  16. Católicos, fidelidade conjugal e AIDS: entre a cruz da doutrina moral e as espadas do cotidiano sexual dos adeptos1

    PubMed Central

    Rios, Luis Felipe; de Aquino, Francisca Luciana; Muñoz-Laboy, Miguel; Oliveira, Cinthia; Parker, Richard

    2009-01-01

    Neste artigo discutimos a visão da Igreja Católica sobre sexualidade na interface com a epidemia do HIV/AIDS. Nossa reflexão está embasada em pesquisa etnográfica que envolveu dois meses de observação participante do cotidiano de católicos de um bairro popular da Região Metropolitana do Recife, além de contar com entrevistas a onze dos leigos engajados nos serviços religiosos da igreja do bairro e a oito sacerdotes que realizam seus trabalhos religiosos em outras localidades. Nelas abordamos diferentes temáticas relacionadas ao enfrentamento da epidemia da AIDS. Nesse contexto, conjugalidade e fidelidade se afiguram como importantes analisadores de como aqueles lidam com a epidemia, em uma variedade de re-descrições práticas e de re-interpretações conceptuais das assertivas do discurso moral religioso – ainda que, muitos impasses permaneçam em aberto em termos das prerrogativas da Igreja e seus possíveis rebatimentos na saúde sexual dos adeptos. PMID:21765650

  17. 78 FR 77614 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-24

    ...-09R1, Amendment 39-16322 (75 FR 30687, June 2, 2010). That AD applies to all Turbomeca S.A. 2B1... Since Existing AD Was Issued Since we issued AD 2007-19-09R1, Amendment 39-16322 (75 FR 30687, June 2... requirements of AD 2007-19- 09R1, Amendment 39-16322 (75 FR 30687, June 2, 2010) but would reduce...

  18. Osteogenic potential of biosilica on human osteoblast-like (SaOS-2) cells.

    PubMed

    Wiens, Matthias; Wang, Xiaohong; Schlossmacher, Ute; Lieberwirth, Ingo; Glasser, Gunnar; Ushijima, Hiroshi; Schröder, Heinz C; Müller, Werner E G

    2010-12-01

    Biosilica is a natural polymer, synthesized by the poriferan enzyme silicatein from monomeric silicate substrates. Biosilica stimulates mineralizing activity and gene expression of SaOS-2 cells. To study its effect on the formation of hydroxyapatite (HA), SaOS-2 cells were grown on different silicatein/biosilica-modified substrates (bone slices, Ca-P-coated coverslips, glass coverslips). Growth on these substrates induced the formation of HA nodules, organized in longitudinal arrays or spherical spots. Nodules of sizes above 1 μm were composed of irregularly arranged HA prism-like nanorods, formed by aggregates of three to eight SaOS-2 cells. Moreover, growth on silicatein/biosilica-modified substrates elicited increased [(3)H]dT incorporation into DNA, indicative of enhanced cell proliferation. Consequently, an in vitro-based bioassay was established to determine the ratio between [(3)H]dT incorporation and HA formation. This ratio was significantly higher for cells that grew on silicatein/biosilica-modified substrates than for cells on Ca-P-coated coverslips or plain glass slips. Hence, we propose that this ratio of in vitro-determined parameters reflects the osteogenic effect of different substrates on bone-forming cells. Finally, qRT-PCR analyses demonstrated that growth of SaOS-2 cells on a silicatein/biosilica matrix upregulated BMP2 (bone morphogenetic protein 2, inducer of bone formation) expression. In contrast, TRAP (tartrate-resistant acid phosphatase, modulator of bone resorption) expression remained unaffected. We conclude that biosilica shows pronounced osteogenicity in vitro, qualifying this material for studies of bone replacement also in vivo. PMID:20725824

  19. 77 FR 43550 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-25

    ... 39-13199 (68 FR 36900, June 20, 2003), for all Turbomeca S.A. Arriel 1A, 1A1, 1A2, 1B, 1C, 1C1, 1C2... Existing AD Was Issued Since we issued AD 2003-12-14, Amendment 39-13199 (68 FR 36900, June 20, 2003), in...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will not...

  20. 76 FR 77446 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-13

    ... Turbomeca S.A. Arrius 2F turboshaft engines with P3 air pipe (first section) part number (P/N) 0 319 71 918... engines with P3 air pipe (first section), P/N 0 319 71 918 0, installed. That AD requires inspections of...) of this AD as follows: (i) At every installation of a RH rear half-wall P/N 0 319 99 824 0 on...

  1. 78 FR 32551 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ...We are superseding an existing airworthiness directive (AD) for Turbomeca S.A. Arrius 2B, 2B1, and 2F turboshaft engines. That AD currently requires replacement of injector manifolds and borescope- inspection of the flame tube and the high-pressure (HP) turbine area for possible damage. This new AD requires, depending on the engine model, repetitive replacements of fuel injection manifolds and......

  2. Design Of A High Efficiency And Reliable S3R SA Regulator

    NASA Astrophysics Data System (ADS)

    Soto, A.

    2011-10-01

    The paper explains the challenges faced in the design of a high efficiency SA shunt switch with low output drop. Two different alternatives are presented, one based on synchronous output rectifier and the other based on having only one diode at the output. Careful design is required to achieve reliability of this critical solution. Both solutions are going to be used in satellites, one for earth observation and the other for a telecommunications GEO platform.

  3. MM-GB/SA rescoring of docking poses in structure-based lead optimization.

    PubMed

    Guimarães, Cristiano R W; Cardozo, Mario

    2008-05-01

    The critical issues in docking include the prediction of the correct binding pose and the accurate estimation of the corresponding binding affinity. Different docking methodologies have all been successful in reproducing the crystallographic binding modes but struggle when predicting the corresponding binding affinities. The aim of this work is to evaluate the performance of the MM-GB/SA rescoring of docking poses in structure-based lead optimization. To accomplish that, a diverse set of pharmaceutically relevant targets, including CDK2, FactorXa, Thrombin, and HIV-RT were selected. The correlation between the MM-GB/SA results and experimental data in all cases is remarkable. It even qualifies this approach as a more attractive alternative for rank-ordering than the Free Energy Perturbation and Thermodynamic Integration methodologies because, while as accurate, it can handle more structurally dissimilar ligands and provides results at a fraction of the computational cost. On the technical side, the benefit of performing a conformational analysis and having an ensemble of conformers to represent each ligand in the unbound state during the MM-GB/SA rescoring procedure was investigated. In addition, the estimation of conformational entropy penalties for the ligands upon binding, computed from the Boltzmann distribution in water, was evaluated and compared to a commonly used approach employed by many docking scoring functions. PMID:18422307

  4. Predicting SA-I mechanoreceptor spike times with a skin-neuron model

    PubMed Central

    Lesniak, Daine R.; Gerling, Gregory J.

    2009-01-01

    Slowly adapting type I (SA-I) mechanoreceptors encode the edges and curvature of touched objects by generating neural spikes in response to indentation of the skin. Beneath this general input-output relationship, models are of great utility for understanding the sub-processes, as SA-I transduction sites are inaccessible to whole-cell recording. This work develops and validates a SA-I skin-receptor model that combines a finite element model of skin mechanics, a sigmoidal function of transduction, and a leaky integrate-and-fire model of neural dynamics. The model produced a R2=0.80 goodness of fit between predicted and observed firing rates for 3 mm and 5 mm grating stimuli. In addition, modulation indices of predicted firing rates for 3 mm and 5 mm gratings are 0.46 and 0.59 respectively, compared to the 0.71 and 0.72 found in vivo. An analysis of predicted first spikes indicates their latency may also be enhanced by edges, as edge proximity shortened first spike latencies by 26.2 and 41.8 ms for the 3 mm and 5 mm gratings, respectively. The model described here bridges the gap between those models that transform sustained indentation to firing rates and those that transform vibration to spike times. PMID:19362097

  5. Production of an extracellular thermohalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2.

    PubMed

    Amoozegar, Mohammad Ali; Salehghamari, Ensieh; Khajeh, Khosro; Kabiri, Mahbube; Naddaf, Saied

    2008-06-01

    Fifty strains of moderately halophilic bacteria were isolated from various salty environments in Iran. A strain designated as SA-2 was shown to be the best producer of extracellular lipase and was selected for further studies. Biochemical and physiological characterization along with 16S rDNA sequence analysis placed SA-2 in the genus Salinivibrio. The optimum salt, pH, temperature and aeration for enzyme production were 0.1 M KCl, pH 8, 35 degrees C and 150 rpm, respectively. The enzyme production was synchronized bacterial growth and reached a maximum level during the early-stationary phase in the basal medium containing 1 M NaCl. Triacylglycerols enhanced lipase production, while carbohydrates had inhibitory effects on it. The maximum lipase activity was obtained at pH 7.5, 50 degrees C and CaCl(2) concentration of 0.01 M. The enzyme was stable at pH range of 7.5-8 and retained 90% of its activity at 80 degrees C for 30 min. Different concentrations of NaNO(3), Na(2)SO(4), KCl and NaCl had no affect on lipase stability for 3 h. These results suggest that the lipase secreted by Salinivibrio sp. strain SA-2 is industrially important from the perspective of its tolerance to a broad temperature range, its moderate thermoactivity and its high tolerance to a wide range of salt concentrations (0-3 M NaCl). PMID:18506896

  6. 1HNMR study of methotrexate serum albumin (MTX SA) binding in rheumatoid arthritis

    NASA Astrophysics Data System (ADS)

    Sułkowska, A.; Maciążek-Jurczyk, M.; Bojko, B.; Równicka, J.; Sułkowski, W. W.

    2008-11-01

    Rheumatoid arthritis (RA) is an immunologically depended disease. It is characterized by a chronic, progressive inflammatory process. Methotrexate (4-amino-10-methylfolic acid, MTX) is the modifying drug used to treat RA. The aim of the presented studies is to determine the low affinity binding site of MTX in bovine (BSA) and human (HSA) serum albumin with the use of proton nuclear magnetic resonance ( 1HNMR) spectroscopy. The analysis of 1HNMR spectra of MTX in the presence of serum albumin (SA) allows us to observe the interactions between aromatic rings of the drug and the rings of amino acids located in the hydrophobic subdomains of the protein. On the basis of the chemical shifts σ [ppm] and the relaxation times T1 [s] of drug protons the hydrophobic interaction between MTX-SA and the stoichiometric molar ratio of the complex was evaluated. This work is a part of a spectroscopic study on MTX-SA interactions [A. Sułkowska, M. Maciążek, J. Równicka, B. Bojko, D. Pentak, W.W. Sułkowski, J. Mol. Struct. 834-836 (2007) 162-169].

  7. PAU-SA: A Synthetic Aperture Interferometric Radiometer Test Bed for Potential Improvements in Future Missions

    PubMed Central

    Ramos-Perez, Isaac; Camps, Adriano; Bosch-Lluis, Xavi; Rodriguez-Alvarez, Nereida; Valencia-Domènech, Enric; Park, Hyuk; Forte, Giuseppe; Vall-llosera, Merce

    2012-01-01

    The Soil Moisture and Ocean Salinity (SMOS) mission is an Earth Explorer Opportunity mission from the European Space Agency (ESA). Its goal is to produce global maps of soil moisture and ocean salinity using the Microwave Imaging Radiometer by Aperture Synthesis (MIRAS). The purpose of the Passive Advanced Unit Synthetic Aperture (PAU-SA) instrument is to study and test some potential improvements that could eventually be implemented in future missions using interferometric radiometers such as the Geoestacionary Atmosferic Sounder (GAS), the Precipitation and All-weather Temperature and Humidity (PATH) and the Geostationary Interferometric Microwave Sounder (GIMS). Both MIRAS and PAU-SA are Y-shaped arrays with uniformly distributed antennas, but the receiver topology and the processing unit are quite different. The purpose of this work is to identify the elements in the MIRAS's design susceptible of improvement and apply them in the PAU-SA instrument demonstrator, to test them in view of these future interferometric radiometer missions. PMID:22969371

  8. Identification of a Unique Ganglioside Binding Loop within Botulinum Neurotoxins C and D-SA

    SciTech Connect

    Karalewitz, Andrew P.-A.; Kroken, Abby R.; Fu, Zhuji; Baldwin, Michael R.; Kim, Jung-Ja P.; Barbieri, Joseph T.

    2010-09-22

    The botulinum neurotoxins (BoNTs) are the most potent protein toxins for humans. There are seven serotypes of BoNTs (A-G) based on a lack of cross antiserum neutralization. BoNTs utilize gangliosides as components of the host receptors for binding and entry into neurons. Members of BoNT/C and BoNT/D serotypes include mosaic toxins that are organized in D/C and C/D toxins. One D/C mosaic toxin, BoNT/D-South Africa (BoNT/D-SA), was not fully neutralized by immunization with BoNT serotype C or D, which stimulated this study. Here the crystal structures of the receptor binding domains of BoNT/C, BoNT/D, and BoNT/D-SA are presented. Biochemical and cell binding studies show that BoNT/C and BoNT/D-SA possess unique mechanisms for ganglioside binding. These studies provide new information about how the BoNTs can enter host cells as well as a basis for understanding the immunological diversity of these neurotoxins.

  9. 77 FR 67675 - Importer of Controlled Substances, Notice of Registration, SA INTL GMBH C/O., Sigma Aldrich Co...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-13

    ..., 2012, 77 FR 50162, SA INTL GMBH C/O., Sigma Aldrich Co., LLC., 3500 Dekalb Street, St. Louis, Missouri... (7437) I Psilocyn (7438) I 1- piperidine I (7470). N-Benzylpiperazine (7493) I Heroin (9200)...

  10. 78 FR 39339 - Importer of Controlled Substances; Notice of Registration; SA INTL GMBH C/O., Sigma Aldrich Co., LLC

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-01

    ..., 78 FR 19015, SA INTL GMBH C/O., Sigma Aldrich Co. LLC., 3500 Dekalb Street, St. Louis, Missouri 63118... Heroin (9200) I Normorphine (9313) I Etonitazene (9624) I Amphetamine (1100) II Methamphetamine (1105)...

  11. Study on rheo-diecasting process of 7075R alloys by SA-EMS melt homogenized treatment

    NASA Astrophysics Data System (ADS)

    Zhihua, G.; Jun, X.; Zhifeng, Z.; Guojun, L.; Mengou, T.

    2016-03-01

    An advanced melt processing technology, spiral annular electromagnetic stirring (SA-EMS) based on the annular electromagnetic stirring (A-EMS) process was developed for manufacturing Al-alloy components with high integrity. The SA-EMS process innovatively combines non-contact electromagnetic stirring and a spiral annular chamber with specially designed profiles to in situ make high quality melt slurry, and intensive forced shearing can be achieved under high shear rate and high intensity of turbulence inside the spiral annular chamber. In this paper, the solidification microstructure and hardness of 7075R alloy die-casting connecting rod conditioned by the SA-EMS melt processing technology were investigated. The results indicate that, the SA-EMS melt processing technology exhibited superior grain refinement and remarkable structure homogeneity. In addition, it can evidently enhance the mechanical performance and reduce the crack tendency.

  12. Extraction and Analysis of Extracellular Polymeric Substances (EPS): Comparison of Methods and EPS Levels in Salmonella pullorum SA 1685

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The extracellular polymeric substances (EPS) production and composition for Salmonella pullorum SA 1685 exposed to artificial groundwater (AGW) has been examined utilizing three EPS extraction methods: lyophilization, ethanol, and sonication. Experiments were carried out to evaluate the robustness...

  13. SA-SSR: a suffix array-based algorithm for exhaustive and efficient SSR discovery in large genetic sequences

    PubMed Central

    Pickett, B. D.; Karlinsey, S. M.; Penrod, C. E.; Cormier, M. J.; Ebbert, M. T. W.; Shiozawa, D. K.; Whipple, C. J.; Ridge, P. G.

    2016-01-01

    Summary: Simple Sequence Repeats (SSRs) are used to address a variety of research questions in a variety of fields (e.g. population genetics, phylogenetics, forensics, etc.), due to their high mutability within and between species. Here, we present an innovative algorithm, SA-SSR, based on suffix and longest common prefix arrays for efficiently detecting SSRs in large sets of sequences. Existing SSR detection applications are hampered by one or more limitations (i.e. speed, accuracy, ease-of-use, etc.). Our algorithm addresses these challenges while being the most comprehensive and correct SSR detection software available. SA-SSR is 100% accurate and detected >1000 more SSRs than the second best algorithm, while offering greater control to the user than any existing software. Availability and implementation: SA-SSR is freely available at http://github.com/ridgelab/SA-SSR Contact: perry.ridge@byu.edu Supplementary information: Supplementary data are available at Bioinformatics online. PMID:27170037

  14. Dr. von Braun, Dr. Mueller, and Dr. Rees at the Firing Room During the SA-6 Launch

    NASA Technical Reports Server (NTRS)

    1964-01-01

    This photograph depicts an intense moment during the SA-6 launch at the Firing Room. Dr. von Braun, Director of the Marshall Space Flight Center (MSFC) is at center; to his left is Dr. George Mueller, Associate Director for Marned Space Flight; and far right is Dr. Eberhard Rees, Director for Research and Development, MSFC. The SA-6, the sixth flight of the Saturn 1 vehicle, launched a S-IV stage (a second stage) and an Apollo boilerplate spacecraft.

  15. The Endophytic Bacterium, Sphingomonas SaMR12, Improves the Potential for Zinc Phytoremediation by Its Host, Sedum alfredii

    PubMed Central

    Zhang, Xincheng; Pan, Fengshan; Yang, Xiaoe; Feng, Ying

    2014-01-01

    The endophytic bacterium, Sphingomonas SaMR12, isolated from Sedum alfredii Hance, appears to increase plant biomass and zinc-extraction from contaminated soil; however, the mechanism by which this occurs is not clear. Here, the ability of SaMR12 to promote zinc extraction and its effects on root morphology and exudation were examined in hydroponics. Zinc treatment increased shoot biomass by 30 to 45%, and by a further 10 to 19% when combined with SaMR12 inoculation. Zinc treatment also increased zinc accumulation modestly and this too was enhanced with SaMR12. Both biomass and zinc levels increased in a dose-dependent manner with significant effects seen at 50 µM zinc and apparent saturation at 500 µM. Zinc and the endophyte also increased levels of auxin but not at 50 µM and zinc increased levels of superoxide and hydrogen peroxide but mainly at 500 µM. As for root morphology, SaMR12 increased root branching, the number of root tips, and surface area. Zinc and SaMR12 also increased the exudation of oxalic acid. For most assays the effects of the endophyte and zinc were additive, with the notable exception of SaMR12 strongly reducing the production of reactive oxygen species at 500 µM zinc. Taken together, these results suggest that the promotion of growth and zinc uptake by exposure to zinc and to SaMR12 are independent of reactive oxygen and do not involve increases in auxin. PMID:25198772

  16. Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus

    SciTech Connect

    Xu, Ling; Sedelnikova, Svetlana E.; Baker, Patrick J.; Rice, David W.

    2006-08-01

    SA0961 is an unknown hypothetical protein from Staphylococcus aureus that can be identified in the Firmicutes division of Gram-positive bacteria. SA0961 was cloned and the protein was overexpressed in Escherichia coli, purified and subsequently crystallized. SA0961 is an unknown hypothetical protein from Staphylococcus aureus that can be identified in the Firmicutes division of Gram-positive bacteria. The gene for the homologue of SA0961 in Bacillus subtilis, ylaN, has been shown to be essential for cell survival, thus identifying the protein encoded by this gene as a potential target for the development of novel antibiotics. SA0961 was cloned and the protein was overexpressed in Escherichia coli, purified and subsequently crystallized. Crystals of selenomethionine-labelled SA0961 diffract to beyond 2.4 Å resolution and belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 31.5, b = 42.7, c = 62.7 Å, β = 92.4° and two molecules in the asymmetric unit. A full structure determination is under way to provide insights into the function of this protein.

  17. Simian adenovirus type 7 (SA-7) induces tumours of nerve-supporting or paraneural cell origin in newborn hamsters.

    PubMed Central

    Ohtaki, S.; Kato, K.

    1989-01-01

    Simian adenovirus type 7 (SA-7) was found to induce tumours originating from nerve-supporting or paraneural cells in newborn hamsters, regardless of injection site or tissues. SA-7 induces glioblastomas characterized by definite localization (subependymal regions) and its main cell type, bipolar spongioblast-like cells, in the brain of hamsters inoculated as newborns. When the eyes of newborn hamsters were directly inoculated, SA-7 failed to induce retinoblastoma (0/27), but retro or peri-bulbar SA-7 tumours frequently occurred in tissues closely related to the peripheral nerve apparatus, including the oculomotor nerve or ciliary ganglion. These tumour cells were situated like stromal cells in these nerve tissues. The histological features of the orbital tumours were similar to those of SA-7-induced subcutaneous tumours but not to brain tumours. In contrast with other hamster brain tumours induced by human adenovirus type 12 or human papova JC virus, medulloepithelioma or medulloblastoma, SA-7 induced tumours exhibit distinctive histological and localization characteristics. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6a Fig. 7 Fig. 8 Fig. 9 Fig. 10 Fig. 11 Fig. 12a PMID:2765394

  18. The contribution of cohesin-SA1 to gene expression and chromatin architecture in two murine tissues.

    PubMed

    Cuadrado, Ana; Remeseiro, Silvia; Graña, Osvaldo; Pisano, David G; Losada, Ana

    2015-03-31

    Cohesin, which in somatic vertebrate cells consists of SMC1, SMC3, RAD21 and either SA1 or SA2, mediates higher-order chromatin organization. To determine how cohesin contributes to the establishment of tissue-specific transcriptional programs, we compared genome-wide cohesin distribution, gene expression and chromatin architecture in cerebral cortex and pancreas from adult mice. More than one third of cohesin binding sites differ between the two tissues and these show reduced overlap with CCCTC-binding factor (CTCF) and are enriched at the regulatory regions of tissue-specific genes. Cohesin/CTCF sites at active enhancers and promoters contain, at least, cohesin-SA1. Analyses of chromatin contacts at the Protocadherin (Pcdh) and Regenerating islet-derived (Reg) gene clusters, mostly expressed in brain and pancreas, respectively, revealed remarkable differences that correlate with the presence of cohesin. We could not detect significant changes in the chromatin contacts at the Pcdh locus when comparing brains from wild-type and SA1 null embryos. In contrast, reduced dosage of SA1 altered the architecture of the Reg locus and decreased the expression of Reg genes in the pancreas of SA1 heterozygous mice. Given the role of Reg proteins in inflammation, such reduction may contribute to the increased incidence of pancreatic cancer observed in these animals. PMID:25735743

  19. Two Redundant Receptor-Like Cytoplasmic Kinases Function Downstream of Pattern Recognition Receptors to Regulate Activation of SA Biosynthesis.

    PubMed

    Kong, Qing; Sun, Tongjun; Qu, Na; Ma, Junling; Li, Meng; Cheng, Yu-Ti; Zhang, Qian; Wu, Di; Zhang, Zhibin; Zhang, Yuelin

    2016-06-01

    Salicylic acid (SA) serves as a critical signaling molecule in plant defense. Two transcription factors, SARD1 and CBP60g, control SA biosynthesis through regulating pathogen-induced expression of Isochorismate Synthase1, which encodes a key enzyme for SA biosynthesis. Here, we report that Pattern-Triggered Immunity Compromised Receptor-like Cytoplasmic Kinase1 (PCRK1) and PCRK2 function as key regulators of SA biosynthesis. In the pcrk1 pcrk2 double mutant, pathogen-induced expression of SARD1, CBP60g, and ICS1 is greatly reduced. The pcrk1 pcrk2 double mutant, but neither of the single mutants, exhibits reduced accumulation of SA and enhanced disease susceptibility to bacterial pathogens. Both PCRK1 and PCRK2 interact with the pattern recognition receptor FLS2, and treatment with pathogen-associated molecular patterns leads to rapid phosphorylation of PCRK2. Our data suggest that PCRK1 and PCRK2 function downstream of pattern recognition receptor in a signal relay leading to the activation of SA biosynthesis. PMID:27208222

  20. The major histocompatibility complex genes impact pain response in DA and DA.1U rats.

    PubMed

    Guo, Yuan; Yao, Fan-Rong; Cao, Dong-Yuan; Li, Li; Wang, Hui-Sheng; Xie, Wen; Zhao, Yan

    2015-08-01

    Our recent studies have shown that the difference in basal pain sensitivity to mechanical and thermal stimulation between Dark-Agouti (DA) rats and a novel congenic DA.1U rats is major histocompatibility complex (MHC) genes dependent. In the present study, we further used DA and DA.1U rats to investigate the role of MHC genes in formalin-induced pain model by behavioral, electrophysiological and immunohistochemical methods. Behavioral results showed biphasic nociceptive behaviors increased significantly following the intraplantar injection of formalin in the hindpaw of DA and DA.1U rats. The main nociceptive behaviors were lifting and licking, especially in DA rats (P<0.001 and P<0.01). The composite pain scores (CPS) in DA rats were significantly higher than those in DA.1U rats in both phases of the formalin test (P<0.01). Electrophysiological results also showed the biphasic increase in discharge rates of C and Aδ fibers of L5 dorsal root in the two strains, and the net change of the discharge rate of DA rats was significantly higher than that of DA.1U rats (P<0.05). The mechanical thresholds decreased after formalin injection in both strains (P<0.01), and the net change in the mechanical threshold in DA was greater than that in DA.1U rats (P<0.05). The expression of RT1-B, representation of MHC class II molecule, in laminae I-II of L4/5 spinal cord in DA rats was significantly higher than that in DA.1U rats in the respective experimental group (P<0.05). These results suggested that both DA and DA.1U rats exhibited nociceptive responses in formalin-induced pain model and DA rats were more sensitive to noxious chemical stimulus than DA.1U rats, indicating that MHC genes might contribute to the difference in pain sensitivity. PMID:25861730

  1. Antitumor effectiveness of different amounts of electrical charge in Ehrlich and fibrosarcoma Sa-37 tumors

    PubMed Central

    Ciria, HC; Quevedo, MS; Cabrales, LB; Bruzón, RP; Salas, MF; Pena, OG; González, TR; López, DS; Flores, JM

    2004-01-01

    Background In vivo studies were conducted to quantify the effectiveness of low-level direct electric current for different amounts of electrical charge and the survival rate in fibrosarcoma Sa-37 and Ehrlich tumors, also the effect of direct electric in Ehrlich tumor was evaluate through the measurements of tumor volume and the peritumoral and tumoral findings. Methods BALB/c male mice, 7–8 week old and 20–22 g weight were used. Ehrlich and fibrosarcoma Sa-37 cell lines, growing in BALB/c mice. Solid and subcutaneous Ehrlich and fibrosarcoma Sa-37 tumors, located dorsolaterally in animals, were initiated by the inoculation of 5 × 106 and 1 × 105 viable tumor cells, respectively. For each type of tumor four groups (one control group and three treated groups) consisting of 10 mice randomly divided were formed. When the tumors reached approximately 0.5 cm3, four platinum electrodes were inserted into their bases. The electric charge delivered to the tumors was varied in the range of 5.5 to 110 C/cm3 for a constant time of 45 minutes. An additional experiment was performed in BALB/c male mice bearing Ehrlich tumor to examine from a histolological point of view the effects of direct electric current. A control group and a treated group with 77 C/cm3 (27.0 C in 0.35 cm3) and 10 mA for 45 min were formed. In this experiment when the tumor volumes reached 0.35 cm3, two anodes and two cathodes were inserted into the base perpendicular to the tumor long axis. Results Significant tumor growth delay and survival rate were achieved after electrotherapy and both were dependent on direct electric current intensity, being more marked in fibrosarcoma Sa-37 tumor. Complete regressions for fibrosarcoma Sa-37 and Ehrlich tumors were observed for electrical charges of 80 and 92 C/cm3, respectively. Histopathological and peritumoral findings in Ehrlich tumor revealed in the treated group marked tumor necrosis, vascular congestion, peritumoral neutrophil infiltration, an acute

  2. Body weight gain during adulthood and uterine myomas: Pró-Saúde Study

    PubMed Central

    Boclin, Karine de Lima Sírio; Torres, Fernanda Pelegrini; Faerstein, Eduardo

    2015-01-01

    ABSTRACT This study intended to investigate whether body weight gain during adulthood is associated with uterine myomas. 1,560 subjects were evaluated in a Pró-Saúde Study. Weight gain was evaluated in a continuous fashion and also in quintiles. Odds ratios and 95% confidence intervals were estimated through logistic regression models that were adjusted for education levels, color/race, body mass indices at age 20, age of menarche, parity, use of oral contraceptive methods, smoking, health insurance, and the Papanicolaou tests. No relevant differences were observed regarding the presence of uterine myomas among weight gain quintiles in that studied population. PMID:26558353

  3. Saturn 5 Launch Vehicle Flight Evaluation Report, SA-513, Skylab 1

    NASA Technical Reports Server (NTRS)

    1973-01-01

    Saturn V SA-513 (Skylab-1) was launched at 13:30:00 Eastern Daylight Time (EDT) on May 14, 1973, from Kennedy Space Center, Complex 39, Pad A. The vehicle lifted off on a launch azimuth of 90 degrees east of north and rolled to a flight azimuth of 40.88 degrees east of north. The launch vehicle successfully placed the Saturn Work Shop in the planned earth orbit. All launch vehicle objectives were accomplished. No launch vehicle failures or anomalies occurred that seriously affected the mission.

  4. Hub and blade structural loads measurements of an SA349/2 helicopter

    NASA Technical Reports Server (NTRS)

    Yamauchi, Gloria K.; Heffernan, Ruth M.; Gaubert, Michel

    1988-01-01

    Data from 23 flight conditions, including level flights ranging from advance ratio mu = 0.14 to 0.37 and steady turning flights from advance ratio mu = 0.26 to 0.35, are presented for an Aerospatiale SA349/2 Gazelle helicopter. The data include hub loads data (for 6 of the 23 conditions), blade structural data at eleven different blade radial stations, and fuselage structural data. All dynamic data are presented as harmonic analysis coefficients (ten harmonics per rotor revolution). The data acquisition and reduction procedures are also documented. Blade structural and inertial properties are provided in addition to control system geometry and properties.

  5. Optical modeling and physical performances evaluations for the JT-60SA ECRF antenna

    NASA Astrophysics Data System (ADS)

    Platania, P.; Figini, L.; Farina, D.; Isayama, A.; Kobayashi, T.; Micheletti, D.; Moriyama, S.; Moro, A.; Sozzi, C.

    2015-12-01

    The purpose of this work is the optical modeling and physical performances evaluations of the JT-60SA ECRF launcher system. The beams have been simulated with the electromagnetic code GRASP® and used as input for ECCD calculations performed with the beam tracing code GRAY, capable of modeling propagation, absorption and current drive of an EC Gaussion beam with general astigmatism. Full details of the optical analysis has been taken into account to model the launched beams. Inductive and advanced reference scenarios has been analysed for physical evaluations in the full poloidal and toroidal steering ranges for two slightly different layouts of the launcher system.

  6. 78 FR 2198 - Airworthiness Directives; Turbomeca S.A. Turboshaft Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-10

    ...We are adopting a new airworthiness directive (AD) for all Turbomeca S.A. Arriel 1A1, 1A2, 1B, 1C, 1C1, 1C2, 1D, 1D1, 1E2, 1K1, 1S, and 1S1 turboshaft engines. This AD requires performing a high gas generator speed (NG) rating vibration check. This AD was prompted by several reports of uncommanded in-flight shutdown on Arriel 1 engines. We are issuing this AD to prevent an uncommanded in-......

  7. [Introduction to a social cartography: the journal Revista de Saúde Pública, 1967 to 1977].

    PubMed

    Santos, Maurici Tadeu Ferreira Dos; Gomes, Mara Helena de Andréa; Silveira, Cássio

    2016-01-26

    Through reliance on a social cartography method based on Karl Mannheim's sociology of knowledge, the article analyzes the first ten issues of Revista de Saúde Pública, published by the University of São Paulo. Articles and editorials are mapped in order to understand the period's transformations. In the realm of the journal's editorial line, tensions are noted between complying with the principles of the institution and international transformations, which demanded an openness to interdisciplinary topics. Special focus is placed on sub-fields, dominated by an epidemiology that was in flux as it absorbed new knowledge in the context of the formation of the field of public health. PMID:27276044

  8. Optical modeling and physical performances evaluations for the JT-60SA ECRF antenna

    SciTech Connect

    Platania, P. Figini, L.; Farina, D.; Micheletti, D.; Moro, A.; Sozzi, C.; Isayama, A.; Kobayashi, T.; Moriyama, S.

    2015-12-10

    The purpose of this work is the optical modeling and physical performances evaluations of the JT-60SA ECRF launcher system. The beams have been simulated with the electromagnetic code GRASP® and used as input for ECCD calculations performed with the beam tracing code GRAY, capable of modeling propagation, absorption and current drive of an EC Gaussion beam with general astigmatism. Full details of the optical analysis has been taken into account to model the launched beams. Inductive and advanced reference scenarios has been analysed for physical evaluations in the full poloidal and toroidal steering ranges for two slightly different layouts of the launcher system.

  9. Synthesis, characterization and antimicrobial applications of zinc oxide nanoparticles loaded gum acacia/poly(SA) hydrogels.

    PubMed

    Bajpai, S K; Jadaun, Mamta; Tiwari, Seema

    2016-11-20

    In this work, zinc oxide nanoparticles were synthesized in-situ within the gum acacia/poly (acrylate) hydrogel network using hydrothermal approach. The synthesized zinc oxide nanoparticles were characterized by Surface plasmon resonance (SPR), X-Ray diffraction (XRD) analysis, Fourier transform infrared (FTIR) spectroscopy and Scanning electron microscopy (SEM). The water absorption behavior of ZnO/GA/poly(SA) hydrogels was investigated in the phosphate buffer saline (PBS) of pH 7.4 at 37°C. The water uptake data were analyzed with the help of various kinetic models. Finally, the antimicrobial action of nanocomposites was studied using E. coli as model bacteria. PMID:27561472

  10. Endophytic bacterium Sphingomonas SaMR12 promotes cadmium accumulation by increasing glutathione biosynthesis in Sedum alfredii Hance.

    PubMed

    Pan, Fengshan; Meng, Qian; Wang, Qiong; Luo, Sha; Chen, Bao; Khan, Kiran Yasmin; Yang, Xiaoe; Feng, Ying

    2016-07-01

    A hydroponic experiment was conducted to verify the effects of inoculation with endophytic bacteria Sphingomonas SaMR12 on root growth, cadmium (Cd) uptake, reactive oxygen species (ROS), antioxidases, glutathione (GSH) and the related gene expression of Sedum alfredii Hance under different levels of Cd such as 0, 10, 25, 100 and 400 μM. The results showed that inoculation of SaMR12 improved Cd accumulation and upregulated glutathione synthase (GS) expression, but slightly reduced malondialdehyde (MDA) concentration and alleviated Cd-induced damage in roots. However it didn't alter the activities of antioxidant enzymes. When Cd concentration exceeded 25 μM, SaMR12 increased the concentration of GSH and the expression level of GSH1. At high Cd treatment levels (100 and 400 μM), SaMR12 significantly reduced H2O2 concentration and enhanced expression level of 1-Cys peroxiredoxin PER1 and ATPS genes. These results indicate that although SaMR12 has no significant effects on antioxidases activities, it reduces H2O2 concentration by enhancing GSH concentration and relevant genes expression, and subsequently improves Cd tolerance and accumulation. PMID:27065458

  11. [Effects of Ca2+ and SA on physiological and photosynthesis of Platycodon grandiflorum under high temperature stress].

    PubMed

    Li, Ke-ni; Wang, Kang-cai; Li, Li; Li, Yu-qing; Duan, Yun-jing

    2015-05-01

    In order to reveal feasibility of different concentrations of Ca2+ and SA on Platycodon grandiflorum under high temperature stress, the effects of Ca2+ on physiological index and related photosynthetic parameters were studied. Pot cultured P. grandiflorum leaves under the same outdoor conditions were sprayed with CaCl2 and SA separately, and then placed in the high incubator [35 degrees C/25 degrees C (day/night), light intensity 3 600 lx], and sprayed with distilled water at 25 degrees C and under high temperature stress were set as the control. The related photosynthesis, relative conductivity, contents of proline, malondialdehyde, soluble protein, activities of SOD and CAT, ASA and GSH content were measured. The results show that the 6 mmol x L(-1) CaCl2 and 1.5 mmol x L(-1) SA enhanced the activities of SOD and CAT, the contents of proline and soluble protein, and effectively reduced the damage of heat stress on cell membrance. At the same time, the exogenous Ca2+ and SA increased the contents of chlorophyll and carotenoid, the efficiency of leaf photosynthesis and ASA and GSH content, and thus effectively resisted the oxidative stress caused by high temperatures, but with the increasing concentration of spraying, P. grandiflorum decreased the ability to resist high temperature stress. In conclusion, the foliage spraying CaCl2 and SA could reduce the damage of high temperature stress on P. grandiflorum leaves. PMID:26390646

  12. Assignment of simian rotavirus SA11 temperature-sensitive mutant groups B and E to genome segments

    SciTech Connect

    Gombold, J.L.; Estes, M.K.; Ramig, R.F.

    1985-05-01

    Recombinant (reassortant) viruses were selected from crosses between temperature-sensitive (ts) mutants of simian rotavirus SA11 and wild-type human rotavirus Wa. The double-stranded genome RNAs of the reassortants were examined by electrophoresis in Tris-glycine-buffered polyacrylamide gels and by dot hybridization with a cloned DNA probe for genome segment 2. Analysis of replacements of genome segments in the reassortants allowed construction of a map correlating genome segments providing functions interchangeable between SA11 and Wa. The reassortants revealed a functional correspondence in order of increasing electrophoretic mobility of genome segments. Analysis of the parental origin of genome segments in ts+ SA11/Wa reassortants derived from the crosses SA11 tsB(339) X Wa and SA11 tsE(1400) X Wa revealed that the group B lesion of tsB(339) was located on genome segment 3 and the group E lesion of tsE(1400) was on segment 8.

  13. Rebalance between 7S and 11S globulins in soybean seeds of differing protein content and 11SA4.

    PubMed

    Yang, A; Yu, X; Zheng, A; James, A T

    2016-11-01

    Protein content and globulin subunit composition of soybean seeds affect the quality of soy foods. In this proteomic study, the protein profile of soybean seeds with high (∼45.5%) or low (∼38.6%) protein content and with or without the glycinin (11S) subunit 11SA4 was examined. 44 unique proteins and their homologues were identified and showed that both protein content and 11SA4 influenced the abundance of a number of proteins. The absence of 11SA4 exerted a greater impact than the protein content, and led to a decreased abundance of glycinin G2/A2B1 and G5/A5A4B3 subunits, which resulted in lower total 11S with a concomitant higher total β-conglycinin (7S). Low protein content was associated with higher glycinin G3/A1aB1b and lower glycinin G4/A5A4B3. Using the proteomic approach, it was demonstrated that 11SA4 deficiency induced compensatory accumulation of 7S globulins and led to a similar total abundance for 7S+11S irrespective of protein content or 11SA4. PMID:27211633

  14. Chemical enhancement of SA7 virus transformation of hamster embryo cells: evaluation by interlaboratory testing of diverse chemicals.

    PubMed

    Hatch, G G; Anderson, T M; Lubet, R A; Kouri, R E; Putman, D L; Cameron, J W; Nims, R W; Most, B; Spalding, J W; Tennant, R W

    1986-01-01

    Twelve chemicals from diverse structural classes were tested under code for their capacity to enhance the transformation of Syrian hamster embryo cells by simian adenovirus SA7 in two independent laboratories. Pretreatment of hamster cells with eight of those chemicals (reserpine, dichlorvos, methapyrilene hydrochloride, benzidine dihydrochloride, diphenylhydantoin, cinnamyl anthranilate, 11-aminoundecanoic acid, and 4,4'-oxydianiline) produced repeatable enhancement of SA7 transformation at two or more consecutive dose levels, which constitutes clear evidence of enhancing activity in this assay. Both toxic and nontoxic doses of each of these chemicals caused enhancement of virus transformation. Two chemicals (2,6-dichloro-p-phenylenediamine and cinnamaldehyde) produced some evidence of enhancing activity (repeatable transformation enhancement at one dose). Dose ranges for cytotoxicity and enhancement of SA7 transformation were similar in both laboratories for all chemicals producing activity. The final two chemicals, chloramphenicol sodium succinate and ethylene thiourea, failed to reproducibly demonstrate either significant cytotoxicity or enhancement of SA7 transformation at concentrations up to 10-20 mM. The test results for these 12 chemicals were combined with the test results for 9 known carcinogens and noncarcinogens in order to evaluate relationships between activity, dose response, and lowest effective enhancing concentration for these compounds, as well as to correlate them with rodent carcinogenesis classifications. The Syrian hamster embryo cell-SA7 system demonstrated reproducible test responses in both intra- and interlaboratory studies and detected 13 out of 15 known rodent carcinogens. PMID:3732194

  15. IM Normae: A Second T Pyx? (Abstract)

    NASA Astrophysics Data System (ADS)

    Patterson, J.; Monard, B.; Warhurst, P.; Myers, G.

    2015-12-01

    (Abstract only) T Pyx is the Galaxy's most famous recurrent nova, erupting to magnitude 6 about every 20 years. For nova hunters and variable-star observers generally, it should be quite easy to discover stars with similar properties. There are probably half a million CVs out to the distance of T Pyx, and most have an underlying structure similar to that of T Pyx: low-mass secondary, fairly massive white dwarf, short orbital period. But of these half million stars, there is no second T Pyx. The star is unique in another way: its orbital period is increasing on a timescale of 300,000 years. Like the proverbial bat out of hell. A 2002 nova eruption nominated a second star for this elite club: IM Nor, a short-orbital-period (2.5 hours) star which previously erupted in 1920. We began a program of time-series photometry to track the shallow eclipses—to test for orbital period change, the other signature of T Pyx resemblance. By 2015 we found this effect: Porb increases on a timescale of 2 million years. Thus, the two stars appear to be blowing themselves apart on a timescale of roughly a million years. This could explain why the stars are so rare: because they are rapidly self-immolating. And that could happen because the classical-nova outburst overwhelms the low-mass secondaries that live in short-period CVs—leading to unstable mass transfer which quickly evaporates the secondary. This implies that all short-Porb classical novae should be "recurrent" (erupting on a timescale of decades). Greater attention to CP Pup (1942), RW UMi (1956), GQ Mus (1983), and V Per (1887) is definitely warranted.

  16. Fluorine substitution enhanced photovoltaic performance of a D-A(1)-D-A(2) copolymer.

    PubMed

    Dang, Dongfeng; Chen, Weichao; Yang, Renqiang; Zhu, Weiguo; Mammo, Wendimagegn; Wang, Ergang

    2013-10-18

    A new alternating donor-acceptor (D-A1-D-A2) copolymer containing two electron-deficient moieties, isoindigo and quinoxaline, was synthesized. The photovoltaic performance of this polymer could be improved by incorporating fluorine atoms into the quinoxaline units, resulting in an efficiency of 6.32%. This result highlights the attractive promise of D-A1-D-A2 copolymers for high-performance bulk heterojunction solar cells. PMID:24000353

  17. An optical design and accuracy estimation for a JT-60SA edge Thomson scattering diagnostic

    NASA Astrophysics Data System (ADS)

    Tojo, H.; Hatae, T.; Hamano, T.; Sakuma, T.; Itami, K.

    2013-12-01

    This paper presents the design of a collection optics used for the Thomson scattering diagnostic in JT-60SA. Good spatial resolution ( ~ 10mm) is necessary for the optics to measure detailed profiles of electron temperature (Te) and density (ne) in the plasma edge region. Six lenses forming a Petzval-like lens are employed in this design. The use of an anomalous dispersion glass material for two lenses suppresses severe chromatic aberration; two flat mirrors prevent interference with the port plug in which the collection optics is to be installed. The resultant spot radius (resolution) at the image for the peripheral region is approximately 0.2 mm. When another collection optics previously designed for core measurements is used simultaneously, the angle of laser polarization must be considered because the two collection optics are to be installed in different port-plugs accessing the vacuum vessel from the cryostat. Otherwise, the measured signal would be degraded, and the scattered spectra would be different from the expected ones. This paper evaluates the scattered spectra with relativistic effects without any approximations. This paper also provides a means to optimize the polarization angle by considering a simulated profile in JT-60SA and contributes to future devices that have limited space for collection optics.

  18. Performance evaluation of the GA/SA hybrid heuristic optimum filter for optical pattern recognition

    NASA Astrophysics Data System (ADS)

    Yeun, Jin S.; Kim, Nam; Pan, Jae Kyung; Kim, R. S.; Um, J. U.; Kim, Sang H.

    1997-04-01

    In this paper, we newly apply a genetic and simulated annealing hybrid heuristic to encode optimal filter for optical pattern recognition. Simulated annealing as a stochastic computational technique allows for finding near globally-minimum-cost solutions with cooling schedule. Using the advantages of a parallelizable genetic algorithm (GA) and a simulated annealing algorithm (SA), the optimum filters are designed and implemented. The filter having 128 multiplied by 128 pixel size consists of the stepped phase that causes the discrete phase delay. The structure of this can be divided into rectangular cells such that each cell imparts a discrete phase delay of 0 approximately equals 2 pi[rad] to the incident wave front. Eight-phase stepped filters that we designed are compared with phase only matched filter and cosine-binary phase only filter. It is deeply focused on investigating the performance of the optimum filter in terms of recognition characteristics on the translation, scale and rotation variations of the image, and discrimination properties against similar images. By GA/SA hybrid heuristic, the optimum filter is realized for high efficiency optical reconstruction in spite of decreasing iteration number needed to encode it by respective algorithms.

  19. Interaction between the Natural Components in Danhong Injection (DHI) with Serum Albumin (SA) and the Influence of the Coexisting Multi-Components on the SaB-BSA Binding System: Fluorescence and Molecular Docking Studies

    PubMed Central

    Hao, Jia; Zhang, Yingyue; Wang, Xingrui; Yan, Huo; Liu, Erwei; Gao, Xiumei

    2015-01-01

    Danhong injection (DHI) is a widely used Chinese Materia Medica standardized product for the clinical treatment of ischemic encephalopathy and coronary heart disease. The bindings of eight natural components in DHI between bovine serum albumin (BSA) were studied by fluorescence spectroscopy technology and molecular docking. According to the results, the quenching process of salvianolic acid B and hydroxysafflor yellow A was a static quenching procedure through the analysis of quenching data by the Stern-Volmer equation, the modified Stern-Volmer equation, and the modified Scatchard equation. Meanwhile, syringin (Syr) enhanced the fluorescence of BSA, and the data were analyzed using the Lineweaver-Burk equation. Molecular docking suggested that all of these natural components bind to serum albumin at the site I location. Further competitive experiments of SaB confirmed the result of molecular docking studies duo to the displacement of warfarin by SaB. Base on these studies, we selected SaB as a research target because it presented the strongest binding ability to BSA and investigated the influence of the multi-components coexisting in DHI on the interaction between the components of the SaB-BSA binding system. The participation of these natural components in DHI affected the interaction between the components of the SaB-BSA system. Therefore, when DHI is used in mammals, SaB is released from serum albumin more quickly than it is used alone. This work would provide a new experiment basis for revealing the scientific principle of compatibility for Traditional Chinese Medicine. PMID:26035712

  20. Novel structure of an ultra-narrow-bandwidth fibre laser based on cascade filters: PGFBG and SA

    NASA Astrophysics Data System (ADS)

    Fan, Zhaojin; Zeng, Xiaodong; Cao, Chanqing; Feng, Zhejun; Lai, Zhi; Dang, Wenjia

    2016-06-01

    An ultra-narrow-linewidth single-longitudinal-mode (SLM) erbium-doped fibre (EDF) ring laser based on cascade filters, which include a phase-gap fibre Bragg grating (PGFBG) and a saturable absorber (SA) is proposed and demonstrated. A PGFBG is used as the first filter to coarsely select the longitudinal mode, the second filter is an SA, which selects the SLM from a few existing longitudinal modes after the first filter by creating a self-writing filter. We measure the linewidth of the fibre ring laser using the delayed self-heterodyne method. The result is approximately 1.2 KHz with a 3 dB linewidth. This fibre laser also has high laser pump power efficiency: the optical to optical efficiency is approximately 15% for a sufficiently long EDF. The stable single linear polarization SLM output of this fibre laser is ensured using polarization-maintaining (PM) devices and the SA.

  1. Autocorrelation descriptor improvements for QSAR: 2DA_Sign and 3DA_Sign.

    PubMed

    Sliwoski, Gregory; Mendenhall, Jeffrey; Meiler, Jens

    2016-03-01

    Quantitative structure-activity relationship (QSAR) is a branch of computer aided drug discovery that relates chemical structures to biological activity. Two well established and related QSAR descriptors are two- and three-dimensional autocorrelation (2DA and 3DA). These descriptors encode the relative position of atoms or atom properties by calculating the separation between atom pairs in terms of number of bonds (2DA) or Euclidean distance (3DA). The sums of all values computed for a given small molecule are collected in a histogram. Atom properties can be added with a coefficient that is the product of atom properties for each pair. This procedure can lead to information loss when signed atom properties are considered such as partial charge. For example, the product of two positive charges is indistinguishable from the product of two equivalent negative charges. In this paper, we present variations of 2DA and 3DA called 2DA_Sign and 3DA_Sign that avoid information loss by splitting unique sign pairs into individual histograms. We evaluate these variations with models trained on nine datasets spanning a range of drug target classes. Both 2DA_Sign and 3DA_Sign significantly increase model performance across all datasets when compared with traditional 2DA and 3DA. Lastly, we find that limiting 3DA_Sign to maximum atom pair distances of 6 Å instead of 12 Å further increases model performance, suggesting that conformational flexibility may hinder performance with longer 3DA descriptors. Consistent with this finding, limiting the number of bonds in 2DA_Sign from 11 to 5 fails to improve performance. PMID:26721261

  2. SA-4-1BBL and monophosphoryl lipid A constitute an efficacious combination adjuvant for cancer vaccines

    PubMed Central

    Srivastava, Abhishek K.; Dinc, Gunes; Sharma, Rajesh K.; Yolcu, Esma S.; Zhao, Hong; Shirwan, Haval

    2014-01-01

    Vaccines based on tumor-associated antigens (TAA) have limited therapeutic efficacy due to their weak immunogenic nature and the various immune evasion mechanisms active in advanced tumors. In an effort to overcome these limitations, we evaluated a combination of the T cell co-stimulatory molecule SA-4-1BBL with the TLR4 agonist monophosphoryl lipid A (MPL) as a novel vaccine adjuvant system. In the TC-1 mouse allograft model of HPV-induced cancer, a single administration of this combination adjuvant with HPV E7 protein caused tumor rejection in all tumor-bearing mice. On its own, SA-4-1BBL outperformed MPL in this setting. Against established tumors, two vaccinations were sufficient to elicit rejection in the majority of mice. In the metastatic model of Lewis lung carcinoma, vaccination of the TAA survivin with SA-4-1BBL/MPL yielded superior efficacy against pulmonary metastases. Therapeutic efficacy of SA-4-1BBL/MPL was achieved in the absence of detectable toxicity, correlating with enhanced DC activation, CD8+ T cell function and an increased intratumoral ratio of CD8+ T effector cells to CD4+FoxP3+ T regulatory cells. Unexpectedly, use of MPL on its own was associated with unfavorable intratumoral ratios of these T cell populations resulting in suboptimal efficacy. The efficacy of MPL monotherapy was restored by depletion of T regulatory cells, whereas eliminating CD8+ T cells abolished the efficacy of its combination with SA-4-1BBL. Mechanistic investigations showed that IFN-γ played a critical role in supporting the therapeutic effect of SA-4-1BBL/MPL. Taken together, our results offer a preclinical proof of concept for the use of a powerful new adjuvant system for TAA-based cancer vaccines. PMID:25252915

  3. Progress in long-pulse production of powerful negative ion beams for JT-60SA and ITER

    NASA Astrophysics Data System (ADS)

    Kojima, A.; Umeda, N.; Hanada, M.; Yoshida, M.; Kashiwagi, M.; Tobari, H.; Watanabe, K.; Akino, N.; Komata, M.; Mogaki, K.; Sasaki, S.; Seki, N.; Nemoto, S.; Shimizu, T.; Endo, Y.; Ohasa, K.; Dairaku, M.; Yamanaka, H.; Grisham, L. R.

    2015-06-01

    Significant progress in the extension of pulse durations of powerful negative ion beams has been made to realize the neutral beam injectors for JT-60SA and ITER. In order to overcome common issues of the long-pulse production/acceleration of negative ion beams in JT-60SA and ITER, new technologies have been developed in the JT-60SA ion source and the MeV accelerator in Japan Atomic Energy Agency. As for the long-pulse production of high-current negative ions for the JT-60SA ion source, the pulse durations have been successfully increased from 30 s at 13 A on JT-60U to 100 s at 15 A by modifying the JT-60SA ion source, which satisfies the required pulse duration of 100 s and 70% of the rated beam current for JT-60SA. This progress was based on the R&D efforts for the temperature control of the plasma grid and uniform negative ion productions with the modified tent-shaped filter field configuration. Moreover, each parameter of the required beam energy, current and pulse has been achieved individually by these R&D efforts. The developed techniques are useful to design the ITER ion source because the sustainment of the caesium coverage in the large extraction area is one of the common issues between JT-60SA and ITER. As for the long-pulse acceleration of high power density beams in the MeV accelerator for ITER, the pulse duration of MeV-class negative ion beams has been extended by more than 2 orders of magnitude by modifying the extraction grid with a high cooling capability and a high transmission of negative ions. A long-pulse acceleration of 60 s has been achieved at 70 MW m-2 (683 keV, 100 A m-2) which has reached the power density of JT-60SA level of 65 MW m-2. No degradations of the voltage holding capability of the acceleration voltage and the beam optics due to the distortion of the acceleration grids have been observed in this power density level. These results are the longest pulse durations of high-current and high-power-density negative ion beams in the

  4. Does long-term use of the Guidelines for Adolescent Preventive Services (GAPS) questionnaires lead to earlier mental health or substance abuse (MH/SA) diagnosis?

    PubMed Central

    Gadomski, Anne M; Scribani, Melissa B; Krupa, Nicole; Jenkins, Paul

    2016-01-01

    Objective Do the GAPS comprehensive screening questionnaires increase detection or shorten time to diagnosis of mental health and substance abuse (MH/SA) disorders? Design Analyses of an administrative database and a prior implementation study to compare change in MH/SA diagnoses pre-GAPS (May 1994 to April 1998) to post-GAPS (May 1999 to April 2003). Setting Rural health network in NY. Participants 8,112 adolescents (13 to 15 years). Intervention GAPS questionnaires routinely administered at annual visits starting May 1999. Outcome Measures Diagnostic codes reflecting MH/SA disorders for outpatient visits subsequent to an index annual visit were enumerated. Population based rates were derived using school enrollment data. Using time series, the rate of MH/SA diagnoses was compared pre- and post-GAPS. Using survival analysis, historical cohorts of adolescents ages 13–15 years who had an annual visit pre- or post-GAPS were constructed to compare the time to MH/SA diagnosis over two 4-year follow up periods. Using a merged database, a sensitivity, specificity, and proportional hazard analysis of the GAPS for MH/SA diagnosis was completed using 297 GAPS questionnaires. Results The rate of MH/SA diagnosis did not change significantly pre- and post-GAPS (p=0.13). There was no difference in time to MH/SA diagnosis post-GAPS (7.0 months) and pre-GAPS (9.0 months, log rank p=0.30). A GAPS with > 3 items positive was associated with an increased risk of any MH/SA diagnosis (H.R. 1.97, p = 0.007). Conclusion Even though related to past or eventual MH/SA diagnosis, GAPS screening did not change the rate of or time to MH/SA diagnosis. N=250 PMID:24488253

  5. Sequence of the hyperplastic genome of the naturally competent Thermus scotoductus SA-01

    PubMed Central

    2011-01-01

    Background Many strains of Thermus have been isolated from hot environments around the world. Thermus scotoductus SA-01 was isolated from fissure water collected 3.2 km below surface in a South African gold mine. The isolate is capable of dissimilatory iron reduction, growth with oxygen and nitrate as terminal electron acceptors and the ability to reduce a variety of metal ions, including gold, chromate and uranium, was demonstrated. The genomes from two different Thermus thermophilus strains have been completed. This paper represents the completed genome from a second Thermus species - T. scotoductus. Results The genome of Thermus scotoductus SA-01 consists of a chromosome of 2,346,803 bp and a small plasmid which, together are about 11% larger than the Thermus thermophilus genomes. The T. thermophilus megaplasmid genes are part of the T. scotoductus chromosome and extensive rearrangement, deletion of nonessential genes and acquisition of gene islands have occurred, leading to a loss of synteny between the chromosomes of T. scotoductus and T. thermophilus. At least nine large inserts of which seven were identified as alien, were found, the most remarkable being a denitrification cluster and two operons relating to the metabolism of phenolics which appear to have been acquired from Meiothermus ruber. The majority of acquired genes are from closely related species of the Deinococcus-Thermus group, and many of the remaining genes are from microorganisms with a thermophilic or hyperthermophilic lifestyle. The natural competence of Thermus scotoductus was confirmed experimentally as expected as most of the proteins of the natural transformation system of Thermus thermophilus are present. Analysis of the metabolic capabilities revealed an extensive energy metabolism with many aerobic and anaerobic respiratory options. An abundance of sensor histidine kinases, response regulators and transporters for a wide variety of compounds are indicative of an oligotrophic lifestyle

  6. Survival of SA11 rotavirus in fresh fruit juices of pineapple, papaya, and honeydew melon.

    PubMed

    Leong, Yap Kok; Xui, Ong Chiaw; Chia, Ong Kien

    2008-05-01

    Survival of rotavirus in fresh fruit juices of papaya (Caraca papaya L.), honeydew melon (Cucumis melo L.), and pineapple (Ananas comosus [L.] Merr.) was studied. Clarified juices were prepared from pulps of ripe fruits and sterilized by ultrafiltration. One milliliter of juice from each fruit was inoculated with 20 microl of 1 x 10(6) PFU of SA11 rotavirus and sampled immediately (0-h exposure) and 1 and 3 h later at 28 degrees C. Mean viral titers in juices of papaya (pH 5.1) and honeydew melon (pH 6.3) at 1 and 3 h were not significantly different from titers at 0-h exposure. Mean viral titers in juices from pineapples with ripening color indices of 3 (pH 3.6) and 6 (pH 3.7) at 1-h exposure (color index 3: 4.0 +/- 1.7 x 10(4); color index 6: 2.3 +/- 0.3 x 10(5)) and 3-h exposure (color index 3: 1.1 +/- 0.4 x 10(4); color index 6:1.3 +/- 0.6 x 10(5)) were significantly lower than titers at 0-h exposure (color index 3: 5.7 +/- 2.9 x 10(5); color index 6: 7.4 +/- 1.3 x 10(5)). Virus titers in pineapple juices of color index 3 were significantly lower than titers of the virus in juices of index 6. In cell culture medium (pH 7.4), SA11 titer remained stable over 3 h at 28 degrees C. However, at pH 3.6, the virus titer was reduced to a level not significantly different from that of the virus in pineapple juice of color index 6 (pH 3.7). In conclusion, papaya and honeydew melon juices, in contrast to pineapple juice, have the potential to transmit rotavirus. Inactivation of SA11 virus in pineapple juice can be possibly attributed to low pH and constituent(s) in the juice. PMID:18522042

  7. Comparison of Xpert MRSA/SA Nasal and MRSA/SA ELITe MGB Assays for Detection of the mecA Gene with Susceptibility Testing Methods for Determination of Methicillin Resistance in Staphylococcus aureus Isolates

    PubMed Central

    Belmekki, Mohamed; Mammeri, Hedi; Hamdad, Farida; Rousseau, Florence; Canarelli, Brigitte

    2013-01-01

    In a series of 82 Staphylococcus strains isolated from culture, 100% were identified as Staphylococcus aureus by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS); 99.9% (77/82) of them were resistant to benzylpenicillin, oxacillin, and cefoxitin, and 6.1% (5/82) were susceptible to methicillin. Xpert MRSA/SA assay results were concordant with the phenotypic results in 76.8% (63/82) of cases and discordant in 23.2% (19/82) of cases. The MRSA/SA ELITe MGB kit results were concordant with phenotypic results in 100% of the cases. When comparing the Xpert MRSA/SA assay results with the MRSA/SA ELITe MGB kit results, 78% (64/82) of the cases were concordant, while 22% (18/82) of the cases were discordant. No statistically significant differences were observed between the two techniques. The PCR protocol that was used to validate the results of these two methods gave the following results: 49 were conventional methicillin-resistant S. aureus (MRSA) isolates (mecA positive and mecALGA251 negative), and 25 were phenotypic MRSA isolates (mecA negative and mecALGA251 positive). PMID:23863569

  8. Perceptions of Nebraska Teachers Regarding the Transition from STARS to NeSA and Its Perceived Influence on the Implementation of a Balanced Assessment System

    ERIC Educational Resources Information Center

    Isom, Jamie

    2012-01-01

    The purpose of this explanatory mixed methods study was to explore the perceptions of Nebraska teachers about their experiences in the transition from STARS to NeSA. The study explored their perceptions of the influence of the transition on implementation of a balanced assessment system. As defined by NDE, a balanced system included NeSA testing,…

  9. Moessbauer analysis of heat affected zones of an SA 508 steel weld

    SciTech Connect

    Kwon, S.J.; Oh, S.J.; Kim, S.; Lee, S.; Kim, J.H.

    1998-12-18

    Microstructure of a heat affected zone (HAZ) in a weld is influenced by many factors such as chemical composition, welding condition, and peak temperature. It is more complex under multi-pass welding because of the repeated heat input. For the analysis of the HAZ microstructure, optical microscope, electron microscope, and X-ray diffraction techniques have been widely used. However, their application is limited since they can hardly make quantitative analysis of HAZ where numerous phases such as martensite, bainite, ferrite, pearlite, austenite, and carbides are co-existing. Moessbauer spectroscopy, in such a case, is particularly useful due to the capability of quantitative analysis on the fraction of each phase. In this study, phases present in the HAZ of an SA 508 steel were identified, and their fractions were quantitatively determined by Moessbauer spectroscopy in conjunction with microscopic observations.

  10. Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics.

    PubMed

    Zorzini, Valentina; Buts, Lieven; Sleutel, Mike; Garcia-Pino, Abel; Talavera, Ariel; Haesaerts, Sarah; De Greve, Henri; Cheung, Ambrose; van Nuland, Nico A J; Loris, Remy

    2014-06-01

    The Staphylococcus aureus genome contains three toxin-antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperatures. Crystallographic and nuclear magnetic resonance (NMR) solution studies show a well-defined dimer. Differences in structure and dynamics between the X-ray and NMR structural ensembles are found in three loop regions, two of which undergo motions that are of functional relevance. The same segments also show functionally relevant dynamics in the distantly related CcdB family despite divergence of function. NMR chemical shift mapping and analysis of residue conservation in the MazF family suggests a conserved mode for the inhibition of MazF by MazE. PMID:24748664

  11. Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics

    PubMed Central

    Zorzini, Valentina; Buts, Lieven; Sleutel, Mike; Garcia-Pino, Abel; Talavera, Ariel; Haesaerts, Sarah; Greve, Henri De; Cheung, Ambrose; van Nuland, Nico A. J.; Loris, Remy

    2014-01-01

    The Staphylococcus aureus genome contains three toxin–antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperatures. Crystallographic and nuclear magnetic resonance (NMR) solution studies show a well-defined dimer. Differences in structure and dynamics between the X-ray and NMR structural ensembles are found in three loop regions, two of which undergo motions that are of functional relevance. The same segments also show functionally relevant dynamics in the distantly related CcdB family despite divergence of function. NMR chemical shift mapping and analysis of residue conservation in the MazF family suggests a conserved mode for the inhibition of MazF by MazE. PMID:24748664

  12. Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9

    PubMed Central

    Feng, Yan; Chen, Cheng; Han, Yuxiang; Chen, Zelin; Lu, Xiaochan; Liang, Fang; Li, Song; Qin, Wei; Lin, Shuo

    2016-01-01

    The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5′-NGG-3′ protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureus (SaCas9), and its KKH variant, successfully induced targeted mutagenesis with high frequency in zebrafish. Confirming previous findings, the SpCas9 variant, VQR, can also induce targeted mutations in zebrafish. Bioinformatics analysis of these new Cas targets suggests that the number of available target sites in the zebrafish genome can be greatly expanded. Collectively, the expanded target repertoire of Cas9 in zebrafish should further facilitate the utility of this organism for genetic studies of vertebrate biology. PMID:27317783

  13. Crack growth in ASME SA-105 grade 2 steel in hydrogen at ambient temperature

    NASA Technical Reports Server (NTRS)

    Walter, R. J.

    1975-01-01

    Cyclic-load crack growth measurements were performed on ASME SA-105 Grade 2 steel specimens exposed to 10,000- and 15,000-psi hydrogen and to 5000-psi helium, all at ambient temperatures. The cyclic-load crack growth rate was found to be faster in high-pressure hydrogen than in helium. Cyclic-load crack growth rates in this steel were not reduced by preloading in air to a stress intensity of 1.5 times the cyclic K sub max in hydrogen. There are indications that holding under load in hydrogen, and loading and unloading in helium retards hydrogen-accelerated cyclic-load crack growth. Cyclic frequency and R (ratio of K sub min/k sub max) were important variables determining crack growth rate. The crack growth rate increased as a logarithm of the cycle duration and decreased with increasing R.

  14. The solvent absorption-extractive distillation (SAED) process for ethanol recovery from gas/vapor streams

    SciTech Connect

    Dale, M.C.

    1993-12-31

    A low energy system for ethanol recovery and dehydration has been developed. This system utilizes a solvent for (1) absorption of ethanol vapors, and then the same solvent for (2) extractive distillation. The ideal solvent for this process would have a high affinity for ethanol, and no affinity for water. Heavy alcohols such as dodecanol, and tridecanol, some phosphorals, and some fatty acids have been determined to meet the desired specifications. These solvents have the effect of making water more volatile than ethanol. Thus, a water stream is taken off initially in the dehydration column, and a near anhydrous ethanol stream is recovered from the ethanol/solvent stripper column. Thus the solvent serves dual uses (1) absorption media, and (2) dehydration media. The SAED process as conceptualized would use a solvent similar to solvents used for direct extractive separation of ethanol from aqueous ethanol solutions.

  15. AmeriFlux BR-Sa1 Santarem-Km67-Primary Forest

    DOE Data Explorer

    Saleska, Scott [University of Arizona

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site BR-Sa1 Santarem-Km67-Primary Forest. Site Description - The LBA Tapajos KM67 Mature Forest site is located in the Tapajos National Forest, a 450,000 ha closed-canopy upland forest in Amazonian Brazil. Bounded by the Tapajos River in the west and highway BR-163 to the east, the tower is located on a flat plateau (or planalto) that extends up to 150 km to the north, south, and east. Within the confines of the National Forest, anthropogenic disturbances are limited to a few small hunting trails. The surrounding stand is classified as primary or "old-growth"" predominantly by its uneven age distribution, emergent trees, numerous epiphytes and abundant large logs. In 2007 falling trees hit the tower guy wires rendering all instrumentation in-operational. After a complete restoration tower measurements resumed in August of 2008.

  16. Modeling flow stress constitutive behavior of SA508-3 steel for nuclear reactor pressure vessels

    NASA Astrophysics Data System (ADS)

    Sun, Mingyue; Hao, Luhan; Li, Shijian; Li, Dianzhong; Li, Yiyi

    2011-11-01

    Based on the measured stress-strain curves under different temperatures and strain rates, a series of flow stress constitutive equations for SA508-3 steel were firstly established through the classical theories on work hardening and softening. The comparison between the experimental and modeling results has confirmed that the established constitutive equations can correctly describe the mechanical responses and microstructural evolutions of the steel under various hot deformation conditions. We further represented a successful industrial application of this model to simulate a forging process for a large conical shell used in a nuclear steam generator, which evidences its practical and promising perspective of our model with an aim of widely promoting the hot plasticity processing for heavy nuclear components of fission reactors.

  17. AEM analyses of SRL 131 glass altered as a function of SA/V

    SciTech Connect

    Mazer, J.J.; Bates, J.K.; Biwer, B.M.; Bradley, C.R.

    1991-01-01

    The goal of the present study was to consider the effects of SVT (svt=surface area-to-volume ratio multiplied by reaction time) scaling on glass/water reactions, particularly with respect to characterizing the alteration layer formed at different values of SVT. Preliminary testing indicated that a Na-rich borosilicate glass, SRL 131, would achieve significant reaction in a relatively short period of time. While 131 glass is not expected to be produced at the defense waste processing facility (DWPF) its composition falls in the range for consideration. The results presented here include a comparison of the solution concentrations and detailed descriptions of the alteration layers that formed, analyzed using Analytical Electron Microscopy (AEM). The examination of solution concentrations and solids changes simultaneously allows a more complete assessment of the effects of surface area to volume ratio (SA/V) on glass reaction. 20 refs., 2 figs., 1 tab.

  18. Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9.

    PubMed

    Feng, Yan; Chen, Cheng; Han, Yuxiang; Chen, Zelin; Lu, Xiaochan; Liang, Fang; Li, Song; Qin, Wei; Lin, Shuo

    2016-01-01

    The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5'-NGG-3' protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureus (SaCas9), and its KKH variant, successfully induced targeted mutagenesis with high frequency in zebrafish. Confirming previous findings, the SpCas9 variant, VQR, can also induce targeted mutations in zebrafish. Bioinformatics analysis of these new Cas targets suggests that the number of available target sites in the zebrafish genome can be greatly expanded. Collectively, the expanded target repertoire of Cas9 in zebrafish should further facilitate the utility of this organism for genetic studies of vertebrate biology. PMID:27317783

  19. Apollo/Saturn 5 Postflight Trajectory - SA-513 Skylab 1 Mission. Tracking and Flight Reconstruction

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The postflight trajectory for the Apollo/Saturn V SA-513 Skylab I flight is presented. An analysis is included of the orbital and powered flight trajectories of the launch vehicle, the orbital trajectory of the spent S-II stage, and the free flight impact trajectory of the expended S-IC stage. Launch vehicle trajectory dependent parameters are provided in earth-fixed launch site, launch vehicle navigation, and geographic polar coordinate systems. The time history of the trajectory parameters for the launch vehicle is presented from guidance reference release to the transfer to ATM control. Tables of significant launch vehicle parameters at engine cutoff, stage separation, and workshop orbit insertion are included. Figures of such parameters as altitude, surface and cross range, and the magnitude of total velocity and acceleration as a function of range time for the powered flight trajectory are given.

  20. SA-4-1BBL as a Novel Adjuvant for the Development of Therapeutic Cancer Vaccines

    PubMed Central

    Sharma, Rajesh K.; Yolcu, Esma S.; Shirwan, Haval

    2016-01-01

    Tumor associated antigen (TAA)-based therapeutic vaccines have great potential as a safe, practical, and cost-efficient alternative to standard treatments for cancer. Clinical efficacy of TAA-based vaccines, however, has yet to be realized and will require adjuvants with pleiotropic functions on immune cells. Such adjuvants need not only to generate/boost T cell responses, but also reverse intrinsic/extrinsic tumor immune evasion mechanisms for therapeutic efficacy. This review focuses on a novel agonistic ligand, SA-4-1BBL, for 4-1BB costimulatory receptor as an adjuvant of choice because of its ability to: i) serve as a vehicle to deliver TAAs to dendritic cells (DCs) for antigen uptake and cross-presentation to CD8+ T cells; ii) augment adaptive Th1 and innate immune responses; and iii) overcome various immune evasion mechanisms, cumulatively translating into therapeutic efficacy in preclinical tumor models. PMID:24521311

  1. Saturn I (SA-1) in Marshall Space Flight Center Test Stand

    NASA Technical Reports Server (NTRS)

    1961-01-01

    On October 27, 1961, the Marshall Space Flight Center (MSFC) and the Nation marked a high point in the 3-year-old Saturn development program when the first Saturn vehicle flew a flawless 215-mile ballistic trajectory from Cape Canaveral, Florida. SA-1 is pictured here, five months before launch, in the MSFC test stand on May 16, 1961. Developed and tested at MSFC under the direction of Dr. Wernher von Braun, SA-1 incorporated a Saturn I, Block I engine. The typical height of a Block I vehicle was approximately 163 feet. and had only one live stage. It consisted of eight tanks, each 70 inches in diameter, clustered around a central tank, 105 inches in diameter. Four of the external tanks were fuel tanks for the RP-1 (kerosene) fuel. The other four, spaced alternately with the fuel tanks, were liquid oxygen tanks, as was the large center tank. All fuel tanks and liquid oxygen tanks drained at the same rates respectively. The thrust for the stage came from eight H-1 engines, each producing a thrust of 165,000 pounds, for a total thrust of over 1,300,000 pounds. The engines were arranged in a double pattern. Four engines, located inboard, were fixed in a square pattern around the stage axis and canted outward slightly, while the remaining four engines were located outboard in a larger square pattern offset 40 degrees from the inner pattern. Unlike the inner engines, each outer engine was gimbaled. That is, each could be swung through an arc. They were gimbaled as a means of steering the rocket, by letting the instrumentation of the rocket correct any deviations of its powered trajectory. The block I required engine gimabling as the only method of guiding and stabilizing the rocket through the lower atmosphere. The upper stages of the Block I rocket reflected the three-stage configuration of the Saturn I vehicle.

  2. Simulation of plasma current ramp-up with reduced magnetic flux consumption in JT-60SA

    NASA Astrophysics Data System (ADS)

    Wakatsuki, T.; Suzuki, T.; Hayashi, N.; Shiraishi, J.; Ide, S.; Takase, Y.

    2015-06-01

    Current ramp-up with reduced central solenoid (CS) flux consumption in JT-60SA has been investigated using an integrated modeling code suite (TOPICS) with a turbulent model (CDBM). The plasma current can be ramped-up from 0.6 MA to 2.1 MA with no additional CS flux consumption if the plasma current is overdriven by neutral-beam-driven and bootstrap current. A time duration required for the current ramp-up without CS flux consumption becomes as long as 150 s in the scenario we have examined. In order to achieve a current overdrive condition from 0.6 MA, the current drive by a lower energy neutral beam (85 keV) is effective. A higher energy neutral beam (500 keV) cannot be used in this early phase with a low central electron density (~2 × 1019 m-3) due to large shine through loss, while it can be effectively used in the later phase. Therefore, the main current driver should be switched from the lower energy neutral beam to the higher energy neutral beam during the current ramp-up phase. As a result of an intensive auxiliary heating, plasma beta (the ratio of the plasma pressure to the magnetic pressure) becomes high. Ideal MHD instabilities of such high beta plasmas have been investigated using a linear ideal MHD stability analysis code (MARG2D). External kink modes which might affect the core plasma can be stabilized during the current ramp-up if there is a perfectly conducting wall at the location of the stabilizing plate and the vacuum vessel of JT-60SA and the plasma has a broader pressure profile with the H-mode pedestal and the internal transport barrier.

  3. Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions.

    PubMed

    Müller, Werner E G; Schröder, Heinz C; Tolba, Emad; Diehl-Seifert, Bärbel; Wang, Xiaohong

    2016-01-01

    Inorganic polyphosphate (polyP) is a physiological energy-rich polymer with multiple phosphoric anhydride bonds. In cells such as bone-forming osteoblasts, glycolysis is the main pathway generating metabolic energy in the form of ATP. In the present study, we show that, under hypoxic culture conditions, the growth/viability of osteoblast-like SaOS-2 cells is not impaired. The addition of polyP to those cells, administered as amorphous calcium polyP nanoparticles (aCa-polyP-NP; approximate size 100 nm), significantly increased the proliferation of the cells. In the presence of polyP, the cells produce significant levels of lactate, the end product of anaerobic glycolysis. Under those conditions, an eight-fold increase in the steady-state level of the membrane-associated carbonic anhydrase IX is found, as well as a six-fold induction of the hypoxia-inducible factor 1. Consequently, biomineral formation onto the SaOS-2 cells decreases under low oxygen tension. If the polyP nanoparticles are added to the cells, the degree of mineralization is enhanced. These changes had been measured also in human mesenchymal stem cells. The assumption that the bicarbonate, generated by the carbonic anhydrase in the presence of polyP under low oxygen, is deposited as a constituent of the bioseeds formed during initial hydroxyapatite formation is corroborated by the identification of carbon besides of calcium, oxygen and phosphorus in the initial biomineral deposit onto the cells using the sensitive technology of high-resolution energy dispersive spectrometry mapping. Based on these data, we conclude that polyP is required for the supply of metabolic energy during bone mineral formation under physiological, hypoxic conditions, acting as a 'metabolic fuel' for the cells to grow. PMID:26453899

  4. A remedy to prevent the P1SA scheme degradation in multidimensional S{sub n} calculations in the presence of material discontinuities

    SciTech Connect

    Voloschenko, A. M.

    2006-07-01

    In the paper a way to prevent the P1 synthetic acceleration (P1SA) scheme degradation in solving small absorption highly heterogeneous (SAHH) multidimensional problems that ensures fast pointwise convergence of the P1SA scheme is discussed. Numerical experiment has shown that the lack of the difference scheme mono-tonicity is the reason of the consistent P1SA scheme degradation in solving SAHH problems. So, improvement of the difference scheme mono-tonicity also improves convergence of the consistent P1SA scheme in solving SAHH problems. In the paper we discuss remedies those improve the difference scheme mono-tonicity without essential degradation in accuracy. We also present results which demonstrate that a suitable choice of the fix-up function in the adaptive weighted diamond difference (AWDD) scheme essentially extends the class of SAHH problems, which can be efficiently accelerated by the consistent P1SA scheme. (authors)

  5. 78 FR 34169 - Notice of Finding That Liberty Reserve S.A. Is a Financial Institution of Primary Money...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... Financial Crimes Enforcement Network Notice of Finding That Liberty Reserve S.A. Is a Financial Institution of Primary Money Laundering Concern AGENCY: Financial Crimes Enforcement Network (FinCEN), Treasury... individuals. Liberty Reserve uses a system of internal accounts and a network of virtual currency...

  6. 75 FR 63048 - Airworthiness Directives; Empresa Brasileira de Aeronautica S.A. (EMBRAER) Model EMB-500 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... (75 FR 45558). That NPRM proposed to correct an unsafe condition for the specified products. The MCAI... Order 12866; (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034... EMBRAER Empresa Brasileira de Aeron utica S.A., Phenom Maintenance Support, Av. Brig. Farina Lima,...

  7. 78 FR 39772 - Huntingdon County Site, FCI USA, LLC, Americas Division, a Subsidiary of FCI SA, Including On...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Huntingdon County Site, FCI USA, LLC, Americas Division, a Subsidiary of FCI SA, Including On-Site Leased Workers From Manpower Inc. and Geodis Wilson Inc., Mount Union, Pennsylvania; Amended Certification...

  8. 78 FR 11555 - Special Conditions: Embraer S.A., Model EMB-550 Airplane; Design Roll Maneuver for Electronic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ... Embraer S.A. EMB-550 airplanes was published in the Federal Register on November 26, 2012 (77 FR 70384...; Design Roll Maneuver for Electronic Flight Controls AGENCY: Federal Aviation Administration (FAA), DOT... design roll maneuver for electronic flight controls, specifically an electronic flight control...

  9. 75 FR 67771 - SA Industries 2, Inc., Formerly Known as Gates Corporation, Fluid Power Division, Including On...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-03

    ... notice was published in the Federal Register on September 22, 2009 (74 FR 48304). At the request of the... Employment and Training Administration SA Industries 2, Inc., Formerly Known as Gates Corporation, Fluid..., applicable to workers of Gates Corporation, Fluid Power Division, a subdivision of Tomkins PLC, including...

  10. 78 FR 14007 - Special Conditions: Embraer S.A., Model EMB-550 Airplanes; Electrical/Electronic Equipment Bay...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-04

    ...These special conditions are issued for the Embraer S.A. Model EMB-550 airplane. This airplane will have novel or unusual design features, specifically distributed electrical and electronic equipment bays in pressurized areas of the airplane. Older transport category airplane electrical/electronic equipment bay installations are located in the lower lobe where the flight crew could determine......