Sample records for darmstadtium 271

  1. 30. BUILDING NO.S 271K AND 271L, VIEW LOOKING SOUTH AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. BUILDING NO.S 271-K AND 271-L, VIEW LOOKING SOUTH AT BACK OF BUILDING NO. 271-L (LEFT), 271-K (MIDDLE) AND ROOF OF BUILDING NO. 271-I (VISIBLE OVER WALKWAY ON RIGHT). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  2. 49 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Purpose. 27.1 Section 27.1 Transportation Office of the Secretary of Transportation NONDISCRIMINATION ON THE BASIS OF DISABILITY IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE General § 27.1 Purpose. The purpose of this part is to...

  3. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission may... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Waiver. 284.271 Section 284.271 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF...

  4. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  5. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  6. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  7. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  8. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  9. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 22 2012-07-01 2012-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must...

  10. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must...

  11. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Published information. 271.3 Section 271.3... AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the... the actions, and the votes taken. (c) Other published information. From time to time, other...

  12. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Rate period. 271.8 Section 271.8... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period. (a) The subsidy rate generally will be set for a 2-year period, or two consecutive 1-year periods...

  13. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Rate period. 271.8 Section 271.8... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period. (a) The subsidy rate generally will be set for a 2-year period, or two consecutive 1-year periods...

  14. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Rate period. 271.8 Section 271.8... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period. (a) The subsidy rate generally will be set for a 2-year period, or two consecutive 1-year periods...

  15. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 21 2011-07-01 2011-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if you...

  16. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 22 2013-07-01 2013-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if you...

  17. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 21 2014-07-01 2014-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if you...

  18. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the Board... of 31 U.S.C. 751. For purposes of the regulations in this part and 4 CFR part 28, a simple majority...

  19. 14 CFR 27.1 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Applicability. 27.1 Section 27.1 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... standards for the issue of type certificates, and changes to those certificates, for normal category...

  20. 14 CFR 27.1 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Applicability. 27.1 Section 27.1 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... standards for the issue of type certificates, and changes to those certificates, for normal category...

  1. 14 CFR 271.7 - Subsidy payout formula.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Subsidy payout formula. 271.7 Section 271.7 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.7 Subsidy...

  2. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  3. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used in...

  4. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  5. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  6. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  7. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  8. 36 CFR 271.1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Definitions. 271.1 Section... BEARâ SYMBOL § 271.1 Definitions. (a) The term Smokey Bear as used in the regulations in this part means... Agriculture in cooperation with the Association of State Foresters and The Advertising Council, or any...

  9. 47 CFR 0.271 - Authority delegated.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Authority delegated. 0.271 Section 0.271 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL COMMISSION ORGANIZATION Delegations of Authority Office... amended at 68 FR 11747, Mar. 12, 2003] Chief, Media Bureau ...

  10. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Discrimination prohibited. 271.9 Section 271.9 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR...

  11. 48 CFR 719.271-5 - Cognizant technical officers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... officers. 719.271-5 Section 719.271-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-5 Cognizant technical officers... cognizant technical officers can affect the opportunity of small business to participate equitably...

  12. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 1984 (HSWA) include any requirement or prohibition which has taken effect under HSWA, such as: (1) All... have taken effect. Note: See §§ 264.1(f)(3), 265.1(c)(4)(ii), 271.3(b), 271.21(e)(2) and 271.121(c)(3... the recycling of hazardous wastes in the coke by-products industry 57 FR 27888 June 22, 1992. Aug. 18...

  13. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 1984 (HSWA) include any requirement or prohibition which has taken effect under HSWA, such as: (1) All... have taken effect. Note: See §§ 264.1(f)(3), 265.1(c)(4)(ii), 271.3(b), 271.21(e)(2) and 271.121(c)(3... the recycling of hazardous wastes in the coke by-products industry 57 FR 27888 June 22, 1992. Aug. 18...

  14. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 1984 (HSWA) include any requirement or prohibition which has taken effect under HSWA, such as: (1) All... have taken effect. Note: See §§ 264.1(f)(3), 265.1(c)(4)(ii), 271.3(b), 271.21(e)(2) and 271.121(c)(3... the recycling of hazardous wastes in the coke by-products industry 57 FR 27888 June 22, 1992. Aug. 18...

  15. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  16. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  17. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  18. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  19. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  20. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  1. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  2. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  3. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  4. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  5. 48 CFR 719.271-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 719.271-1 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-1 General. The purpose of this section is to prescribe responsibilities and procedures for carrying out the small business program policy set forth in 219.270, and in...

  6. 48 CFR 719.271-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 719.271-1 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-1 General. The purpose of this section is to prescribe responsibilities and procedures for carrying out the small business program policy set forth in 219.270, and in...

  7. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Government Technical Monitor (GTM). 642.271 Section 642.271 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACT MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The...

  8. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  9. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  10. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  11. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  12. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Rate period. 271.8 Section 271.8 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... place essential air service level; or (5) The uncertainties of the market or other circumstances warrant...

  13. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  14. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  15. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  16. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  17. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  18. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  19. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  20. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  1. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  2. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Title. 1352.271-80 Section... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following clause: Title (APR 2010) (a) Title to all materials and...

  3. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-87 Changes—ship repair. As prescribed in 48 CFR 1371.118,...

  4. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations § 115.271...

  5. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations § 115.271...

  6. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations § 115.271...

  7. 48 CFR 719.271 - Agency program direction and operation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Agency program direction and operation. 719.271 Section 719.271 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271 Agency program direction and...

  8. 48 CFR 719.271 - Agency program direction and operation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Agency program direction and operation. 719.271 Section 719.271 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271 Agency program direction and...

  9. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  10. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  11. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  12. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  13. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  14. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... activities. 719.271-4 Section 719.271-4 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities. In order for the agency small business program to be effective, the active support of top management...

  15. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 3 2010-07-01 2010-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  16. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 3 2011-07-01 2011-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  17. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 3 2013-07-01 2013-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  18. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 3 2012-07-01 2012-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  19. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 3 2014-07-01 2014-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  20. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  1. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  2. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  3. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  4. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  5. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells.

    PubMed

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-07-29

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells' tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC.

  6. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells

    PubMed Central

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-01-01

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells’ tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC. PMID:27469492

  7. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  8. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  9. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  10. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  11. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  12. CD271 Defines a Stem Cell-Like Population in Hypopharyngeal Cancer

    PubMed Central

    Imai, Takayuki; Tamai, Keiichi; Oizumi, Sayuri; Oyama, Kyoko; Yamaguchi, Kazunori; Sato, Ikuro; Satoh, Kennichi; Matsuura, Kazuto; Saijo, Shigeru; Sugamura, Kazuo; Tanaka, Nobuyuki

    2013-01-01

    Cancer stem cells contribute to the malignant phenotypes of a variety of cancers, but markers to identify human hypopharyngeal cancer (HPC) stem cells remain poorly understood. Here, we report that the CD271+ population sorted from xenotransplanted HPCs possesses an enhanced tumor-initiating capability in immunodeficient mice. Tumors generated from the CD271+ cells contained both CD271+ and CD271− cells, indicating that the population could undergo differentiation. Immunohistological analyses of the tumors revealed that the CD271+ cells localized to a perivascular niche near CD34+ vasculature, to invasive fronts, and to the basal layer. In accordance with these characteristics, a stemness marker, Nanog, and matrix metalloproteinases (MMPs), which are implicated in cancer invasion, were significantly up-regulated in the CD271+ compared to the CD271− cell population. Furthermore, using primary HPC specimens, we demonstrated that high CD271 expression was correlated with a poor prognosis for patients. Taken together, our findings indicate that CD271 is a novel marker for HPC stem-like cells and for HPC prognosis. PMID:23626764

  13. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  14. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  15. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  16. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  17. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of forest...

  18. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of forest...

  19. 40 CFR 271.7 - Attorney General's statement.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Attorney General's statement. 271.7... Authorization § 271.7 Attorney General's statement. (a) Any State that seeks to administer a program under this subpart shall submit a statement from the State Attorney General (or the attorney for those State agencies...

  20. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State...

  1. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.11 Requirements for transporters of hazardous wastes. (a) The State...

  2. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.11 Requirements for transporters of hazardous wastes. (a) The State...

  3. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State...

  4. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State...

  5. 48 CFR 1352.271-86 - Lay days.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Lay days. 1352.271-86... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-86 Lay days. As prescribed in 48 CFR 1371.117, insert the following clause: Lay Days (APR 2010) (a) A lay day is defined as an...

  6. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  7. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  8. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  9. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  10. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  11. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  12. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  13. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  14. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  15. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  16. 40 CFR 271.3 - Availability of final authorization.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... authority of the Hazardous Waste Electronic Manifest Establishment Act: (i) Shall take effect in each State.... 271.3 Section 271.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final...

  17. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and copied during normal business hours at the following locations: Office of State Programs... 29 Labor 9 2010-07-01 2010-07-01 false Where the plan may be inspected. 1952.271 Section 1952.271..., DEPARTMENT OF LABOR (CONTINUED) APPROVED STATE PLANS FOR ENFORCEMENT OF STATE STANDARDS Vermont § 1952.271...

  18. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE...

  19. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE...

  20. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  1. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  2. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  3. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  4. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification for...

  5. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification for...

  6. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  7. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  8. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  9. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  10. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  11. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Requirements (AIR)-growth work 1352.271-72 Section 1352.271-72 Federal Acquisition Regulations System... Clauses 1352.271-72 Additional Item Requirements (AIR)—growth work As prescribed in 48 CFR 1371.103, insert the following clause: Additional Item Requirements (Air)—Growth Work (APR 2010) (a) This clause...

  12. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  13. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  14. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Facility pest management practice standard. 205.271... MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED... Requirements § 205.271 Facility pest management practice standard. (a) The producer or handler of an organic...

  15. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Records available to the public on request. 271... RULES REGARDING AVAILABILITY OF INFORMATION § 271.5 Records available to the public on request. (a) Types of records made available. All records of the Committee that are not available under §§ 271.3 and...

  16. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Records available to the public on request. 271... RULES REGARDING AVAILABILITY OF INFORMATION § 271.5 Records available to the public on request. (a) Types of records made available. All records of the Committee that are not available under §§ 271.3 and...

  17. 14 CFR 125.271 - Emergency and emergency evacuation duties.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Emergency and emergency evacuation duties. 125.271 Section 125.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... CERTIFICATION AND OPERATIONS: AIRPLANES HAVING A SEATING CAPACITY OF 20 OR MORE PASSENGERS OR A MAXIMUM PAYLOAD...

  18. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Heads of contracting activities. 719.271-4 Section 719.271-4 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL... decisions in cases resulting from non-acceptances by their contracting officers of set-aside recommendations...

  19. 47 CFR 80.271 - Technical requirements for portable survival craft radiotelephone transceivers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... craft radiotelephone transceivers. 80.271 Section 80.271 Telecommunication FEDERAL COMMUNICATIONS... Authorization for Compulsory Ships § 80.271 Technical requirements for portable survival craft radiotelephone transceivers. (a) Portable survival craft radiotelephone transceivers must comply with the following: (1) The...

  20. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  1. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  2. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  3. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  4. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... minimize the impacts of use as a dust suppressant on the environment. (h)(1) Unless otherwise provided in... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Requirements for used oil management. 271.26 Section 271.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID...

  5. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... minimize the impacts of use as a dust suppressant on the environment. (h)(1) Unless otherwise provided in... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Requirements for used oil management. 271.26 Section 271.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID...

  6. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... minimize the impacts of use as a dust suppressant on the environment. (h)(1) Unless otherwise provided in... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Requirements for used oil management. 271.26 Section 271.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID...

  7. 40 CFR 271.4 - Consistency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.4... hazardous waste programs which have received final authorization under this part. (a) Any aspect of the...

  8. 40 CFR 271.4 - Consistency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.4... hazardous waste programs which have received final authorization under this part. (a) Any aspect of the...

  9. 40 CFR 271.4 - Consistency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.4... hazardous waste programs which have received final authorization under this part. (a) Any aspect of the...

  10. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271...

  11. 15 CFR 904.271 - Initial decision.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES Hearing and Appeal Procedures Decision § 904.271 Initial decision. (a) After expiration of the period...

  12. 15 CFR 904.271 - Initial decision.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES Hearing and Appeal Procedures Decision § 904.271 Initial decision. (a) After expiration of the period...

  13. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  14. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  15. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  16. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  17. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  18. 14 CFR 271.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.1 Purpose... establishing the fair and reasonable amount of compensation needed to ensure the continuation of essential air...

  19. 14 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.2 Definitions... compensation under Subchapter II of Chapter 417 of the Statute. Essential air service is that air...

  20. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... that such wastes do not present a hazard to human health or the environment. These requirements shall... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements...

  1. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... that such wastes do not present a hazard to human health or the environment. These requirements shall... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements...

  2. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... that such wastes do not present a hazard to human health or the environment. These requirements shall... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements...

  3. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.6 Complaint... Jackson Blvd., 20th Floor, Chicago, IL 60604-3507. (iv) For Arkansas, Louisiana, New Mexico, Oklahoma, and...

  4. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TREASURY LIQUORS BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as...

  5. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TREASURY LIQUORS BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as...

  6. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission may...

  7. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Committee includes rules, statements, decisions, minutes, memoranda, letters, reports, transcripts, accounts... INFORMATION § 271.2 Definitions. (a) Board means the Board of Governors of the Federal Reserve System... from or on behalf of one who seeks information for a use or purpose that furthers the commercial, trade...

  8. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the transportation and delivery of hazardous waste shipments. No other electronic signature other than... in connection with the signing of an electronic manifest. (3) Ensure that all wastes offered for... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED...

  9. 48 CFR 1516.405-271 - Limitations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1516.405-271 Limitations. (a) No award... satisfactory or excellent. All award fee plans shall disclose to offerors the numerical rating necessary to be...

  10. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  11. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  12. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  13. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  14. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  15. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  16. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  17. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  18. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  19. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to human...

  20. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to human...

  1. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware of...

  2. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  3. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  4. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  5. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  6. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  7. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.5 Carrier revenues. (a) The projected passenger revenue for a carrier providing essential air service at an eligible...

  8. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.4 Carrier costs. (a) The reasonable costs projected for a carrier providing essential air service at an eligible...

  9. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.6 Profit element. The reasonable return for a carrier for providing essential air service at an eligible place...

  10. 20 CFR 404.271 - When automatic cost-of-living increases apply.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false When automatic cost-of-living increases apply. 404.271 Section 404.271 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Computing Primary Insurance Amounts Cost-Of-Living Increases § 404...

  11. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period... place essential air service level; or (5) The uncertainties of the market or other circumstances warrant...

  12. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System (ISMLS) § 171.271 Installation requirements. (a) The ISMLS facility must be permanent in nature, located... codes, FCC licensing requirements, and paragraphs (a) and (c) of § 171.261. (b) The ISMLS facility must...

  13. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System (ISMLS) § 171.271 Installation requirements. (a) The ISMLS facility must be permanent in nature, located... codes, FCC licensing requirements, and paragraphs (a) and (c) of § 171.261. (b) The ISMLS facility must...

  14. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System (ISMLS) § 171.271 Installation requirements. (a) The ISMLS facility must be permanent in nature, located... codes, FCC licensing requirements, and paragraphs (a) and (c) of § 171.261. (b) The ISMLS facility must...

  15. CD271+ cells are diagnostic and prognostic and exhibit elevated MAPK activity in SHH medulloblastoma.

    PubMed

    Liang, Lisa; Morrison, Ludivine Coudière; Tatari, Nazanin; Stromecki, Margaret; Fresnoza, Agnes; Porter, Christopher J; Del Bigio, Marc R; Hawkins, Cynthia; Chan, Jennifer A; Ryken, Timothy C; Taylor, Michael D; Ramaswamy, Vijay; Werbowetski-Ogilvie, Tamra E

    2018-06-21

    The extensive heterogeneity both between and within the medulloblastoma (MB) subgroups underscores a critical need for variant-specific biomarkers and therapeutic strategies. We previously identified a role for the CD271/p75 neurotrophin receptor (p75NTR) in regulating stem/progenitor cells in the SHH MB subgroup. Here we demonstrate the utility of CD271 as a novel diagnostic and prognostic marker for SHH MB using immunohistochemical analysis and transcriptome data across 763 primary tumors. RNA sequencing of CD271+ and CD271- cells revealed molecularly distinct, co-existing cellular subsets both in vitro and in vivo. MAPK/ERK signaling was upregulated in the CD271+ population, and inhibiting this pathway reduced endogenous CD271 levels, stem/progenitor cell proliferation, and cell survival as well as cell migration in vitro. Treatment with the MEK inhibitor selumetinib extended survival and reduced CD271 levels in vivo; whereas, treatment with vismodegib, a well-known smoothened (SMO) inhibitor currently in clinical trials for the treatment of recurrent SHH medulloblastoma, had no significant effect in our models. Our study demonstrates the clinical utility of CD271 as both a diagnostic and prognostic tool for SHH MB tumors and reveals a novel role for MEK inhibitors in targeting CD271+ SHH MB cells. Copyright ©2018, American Association for Cancer Research.

  16. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... from the Publications Services of the Federal Reserve Board, 20th Street and Constitution Avenue, N.W... Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the...

  17. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... from the Publications Services of the Federal Reserve Board, 20th Street and Constitution Avenue, N.W... Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the...

  18. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  19. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  20. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  1. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  2. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  3. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System, 20th Street and Constitution Avenue, N.W., Washington, D.C. 20551 (the pedestrian entrance is on C...

  4. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System, 20th Street and Constitution Avenue, N.W., Washington, D.C. 20551 (the pedestrian entrance is on C...

  5. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System, 20th Street and Constitution Avenue, N.W., Washington, D.C. 20551 (the pedestrian entrance is on C...

  6. 48 CFR 1352.271-71 - Method of payment and invoicing instructions for ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Method of payment and invoicing instructions for ship repair. 1352.271-71 Section 1352.271-71 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-71 Method of...

  7. 30 CFR 550.271 - For what reasons will BOEM disapprove the DPP or DOCD?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false For what reasons will BOEM disapprove the DPP or DOCD? 550.271 Section 550.271 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF... Information Review and Decision Process for the Dpp Or Docd § 550.271 For what reasons will BOEM disapprove...

  8. 30 CFR 550.271 - For what reasons will BOEM disapprove the DPP or DOCD?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false For what reasons will BOEM disapprove the DPP or DOCD? 550.271 Section 550.271 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF... Information Review and Decision Process for the Dpp Or Docd § 550.271 For what reasons will BOEM disapprove...

  9. 47 CFR 0.271 - Authority delegated.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... of Strategic Planning and Policy Analysis § 0.271 Authority delegated. (a) Insofar as authority is... status, the Chief, Office of Strategic Planning and Policy Analysis is delegated authority to deny... Strategic Planning and Policy Analysis has primary authority. (b) [Reserved] [45 FR 10347, Feb. 15, 1980, as...

  10. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  11. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  12. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  13. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  14. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  15. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government..., or because of special skills or knowledge necessary for monitoring the contractor's work. The...

  16. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government..., or because of special skills or knowledge necessary for monitoring the contractor's work. The...

  17. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government..., or because of special skills or knowledge necessary for monitoring the contractor's work. The...

  18. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall...

  19. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall...

  20. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  1. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  2. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  3. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  4. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  5. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  6. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  7. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  8. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  9. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  10. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  11. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  12. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  13. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  14. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  15. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Commercial license. 271.4...

  16. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Commercial license. 271.4...

  17. 14 CFR 271.3 - Carrier subsidy need.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.3 Carrier subsidy need. In establishing the subsidy for an air carrier providing essential air service at an...

  18. 27 CFR 19.271 - Construction of buildings

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Construction of buildings..., DEPARTMENT OF THE TREASURY LIQUORS DISTILLED SPIRITS PLANTS Construction, Equipment and Security § 19.271 Construction of buildings Buildings in which spirits, denatured spirits, articles, or wines are produced...

  19. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  20. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  1. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  2. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  3. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  4. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  5. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  6. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  7. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  8. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  9. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a...), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining the...

  10. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a...), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining the...

  11. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... cetacean species (not including mother/calf pairs, unless of species of concern listed in § 216.271(b)(1.... (ii) A single individual or mother/calf pair of any of the following marine mammals of concern: Beaked...

  12. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... fostering public information in the prevention of forest fires. All authorities and licenses granted under... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Power to revoke. 271.7...

  13. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... fostering public information in the prevention of forest fires. All authorities and licenses granted under... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Power to revoke. 271.7...

  14. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY THE SECRETARY OF AGRICULTURE AND GENERAL OFFICERS OF THE DEPARTMENT Delegations of Authority by the...

  15. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY THE SECRETARY OF AGRICULTURE AND GENERAL OFFICERS OF THE DEPARTMENT Delegations of Authority by the...

  16. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY THE SECRETARY OF AGRICULTURE AND GENERAL OFFICERS OF THE DEPARTMENT Delegations of Authority by the...

  17. Fluorocycline TP-271 Is Potent against Complicated Community-Acquired Bacterial Pneumonia Pathogens

    PubMed Central

    Fyfe, Corey; O’Brien, William; Hackel, Meredith; Minyard, Mary Beth; Waites, Ken B.; Dubois, Jacques; Murphy, Timothy M.; Slee, Andrew M.; Weiss, William J.; Sutcliffe, Joyce A.

    2017-01-01

    ABSTRACT TP-271 is a novel, fully synthetic fluorocycline antibiotic in clinical development for the treatment of respiratory infections caused by susceptible and multidrug-resistant pathogens. TP-271 was active in MIC assays against key community respiratory Gram-positive and Gram-negative pathogens, including Streptococcus pneumoniae (MIC90 = 0.03 µg/ml), methicillin-sensitive Staphylococcus aureus (MSSA; MIC90 = 0.25 µg/ml), methicillin-resistant S. aureus (MRSA; MIC90 = 0.12 µg/ml), Streptococcus pyogenes (MIC90 = 0.03 µg/ml), Haemophilus influenzae (MIC90 = 0.12 µg/ml), and Moraxella catarrhalis (MIC90 ≤0.016 µg/ml). TP-271 showed activity (MIC90 = 0.12 µg/ml) against community-acquired MRSA expressing Panton-Valentine leukocidin (PVL). MIC90 values against Mycoplasma pneumoniae, Legionella pneumophila, and Chlamydia pneumoniae were 0.004, 1, and 4 µg/ml, respectively. TP-271 was efficacious in neutropenic and immunocompetent animal pneumonia models, generally showing, compared to the burden at the start of dosing, ~2 to 5 log10 CFU reductions against MRSA, S. pneumoniae, and H. influenzae infections when given intravenously (i.v.) and ~1 to 4 log10 CFU reductions when given orally (p.o.). TP-271 was potent against key community-acquired bacterial pneumonia (CABP) pathogens and was minimally affected, or unaffected, by tetracycline-specific resistance mechanisms and fluoroquinolone or macrolide drug resistance phenotypes. IMPORTANCE Rising resistance rates for macrolides, fluoroquinolones, and β-lactams in the most common pathogens associated with community-acquired bacterial pneumonia (CABP) are of concern, especially for cases of moderate to severe infections in vulnerable populations such as the very young and the elderly. New antibiotics that are active against multidrug-resistant Streptococcus pneumoniae and Staphylococcus aureus are needed for use in the empirical treatment of the most severe forms of this disease. TP-271 is a promising

  18. 48 CFR 719.271-5 - Cognizant technical officers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-5 Cognizant technical officers. Since the procurement process starts with the establishment of a requirement, the actions of the cognizant technical officers can affect the opportunity of small business to participate equitably...

  19. Single-Stage Preparation of Human Cartilage Grafts Generated from Bone Marrow-Derived CD271+ Mononuclear Cells.

    PubMed

    Petters, Oliver; Schmidt, Christian; Henkelmann, Ralf; Pieroh, Philipp; Hütter, Gero; Marquass, Bastian; Aust, Gabriela; Schulz, Ronny M

    2018-04-15

    Due to the limited self-healing capacity of articular cartilage, innovative, regenerative approaches are of particular interest. The use of two-stage procedures utilizing in vitro-expanded mesenchymal stromal cells (MSCs) from various cell sources requires good manufacturing practice-compliant production, a process with high demands on time, staffing, and financial resources. In contrast, one- stage procedures are directly available, but need a safe enrichment of potent MSCs. CD271 is a surface marker known to marking the majority of native MSCs in bone marrow (BM). In this study, the feasibility of generating a single-stage cartilage graft of enriched CD271 + BM-derived mononuclear cells (MNCs) without in vitro monolayer expansion from eight healthy donors was investigated. Cartilage grafts were generated by magnetic-activated cell sorting and separated cells were directly transferred into collagen type I hydrogels, followed by 3D proliferation and differentiation period of CD271 + , CD271 - , or unseparated MNCs. CD271 + MNCs showed the highest proliferation rate, cell viability, sulfated glycosaminoglycan deposition, and cartilage marker expression compared to the CD271 - or unseparated MNC fractions in 3D culture. Analysis according to the minimal criteria of the International Society for Cellular Therapy highlighted a 66.8-fold enrichment of fibroblast colony-forming units in CD271 + MNCs and the only fulfillment of the MSC marker profile compared to unseparated MNCs. In summary, CD271 + MNCs are capable of generating adequate articular cartilage grafts presenting high cell viability and notable chondrogenic matrix deposition in a CE-marked collagen type I hydrogel, which can obviate the need for an initial monolayer expansion.

  20. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor (GTM...

  1. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  2. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  3. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  4. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  5. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  6. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  7. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  8. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  9. 36 CFR 271.8 - Consultation with Association of State Foresters and the Advertising Council.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Consultation with Association of State Foresters and the Advertising Council. 271.8 Section 271.8 Parks, Forests, and Public... Association of State Foresters and the Advertising Council. These regulations in this part have been issued...

  10. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of the...

  11. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of the...

  12. Functional analysis of potassium channels in Kv7.2 G271V mutant causing early onset familial epilepsy.

    PubMed

    Wang, Juanjuan; Li, Yuan; Hui, Zhiyan; Cao, Min; Shi, Ruiming; Zhang, Wei; Geng, Limeng; Zhou, Xihui

    2015-08-07

    Kv7 (KCNQ) channels underlying a class of voltage-gated K+ current are best known for regulating neuronal excitability. The first glycine (G) residue in the pore helix of Kv7.2 (KCNQ2) subunit is highly conserved among different classes of Kv7 channel family. A missense mutation causing the replacement of the corresponding G residues with a valine (p.G271V) in Kv7.2 was found in a large, four-generation pedigree. Here, we set out to examine the molecular pathomechanism of G271V mutants using patch clamp technology combined with biochemical and immunocytochemical techniques in transiently transfected human embryonic kidney (HEK) 293 cells. The expression of Kv7.2 protein had the same intensity for both wild type (WT) and G271V. In transfected HEK cells, G271V mutants induced large depolarizing shifts of the conductance-voltage relationships and marked slowing of current activation kinetics compared to WT. In addition, G271V mutants abolished currents in homomeric channels, and resulted in about 50% reduction of current in Kv7.2/G271V/Kv7.3 heteromultimeric condition, indicating a more severe functional defect. To test for G271V mutant channel expression in surface membrane, we performed fluorescence confocal microscopy imaging, which revealed no differences between the mutant and WT, suggesting that G271V channels fail to open in response to depolarization even though they are present in the membrane. Furthermore, pharmacologic intervention experiments revealed that upon specific incubation of transfected HEK 293 cells expressing G271V heteromultimeric channels in presence of Kv7 channel enhancer retigabine (ezogabine), the potassium currents increased significantly, suggesting the potential of retigabine as gene-specific therapy. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  14. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  15. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  16. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  17. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  18. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review Form...

  19. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c...

  20. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for used oil management... Final Authorization § 271.26 Requirements for used oil management. The State shall have standards for used oil management which are equivalent to 40 CFR part 279. These standards shall include: (a...

  1. Immunohistochemical CD271 expression correlates with melanoma progress in a case-control study.

    PubMed

    Nielsen, Patricia Switten; Riber-Hansen, Rikke; Steiniche, Torben

    2018-06-01

    Putative cancer stem cell (CSC) markers have arisen from melanoma mouse and in vitro models, but their expression in paraffin embedded patient samples relative to clinical outcome remains largely unexplored. Rather than cells of the tumour bulk, conceivably, CSC drive tumour progression. Accordingly, complete eradication may prevent melanoma relapse. Because elevated tumour-cell proliferation is an established indicator of aggressive disease, this study aimed to investigate the correlation between melanoma recurrence and proliferation of putative CSC that express CD271, CD166, or CD20. Additionally, the expression of these markers was studied in naevi, melanomas, and their recurrence. In melanoma patients, 30 with relapse (cases) and 30 without (controls) were matched for tumour thickness, ulceration, Clark level, subtype, site, gender, and age. One paraffin-embedded section of the patients' primary melanoma (n = 60), relapse (n = 21), and naevus (n = 17) were immunohistochemically double-stained for Ki-67/MART1 and single-stained for CD271, CD166, and CD20. Their whole slide images were aligned as virtual quadruple stains. Image analysis established proliferation indices of each putative stem cell marker and the tumour bulk in addition to the markers' percentage level in tumour areas and the epidermis. In cases vs controls, median dermal proliferation indices (no./mm 2 ) were 211 vs 103 (p = 0.04) for CD271, 512 vs 227 (p = 0.3) for CD166, 184 vs 97 (p = 0.3) for CD20, and 95 vs 103 (p = 0.6) for the tumour bulk. Of additional interest, epidermal CD271 + keratinocytes totalled 8.8% in naevi and 0.98% in melanomas (p = 0.0007). Even though differences between naevi and melanomas also were observed for CD166 in both the epidermis (p = 0.002) and dermis (p = 0.006), they were visually less apparent. CD20 + MART1 + cells were absent in half of the melanomas, and all naevi and relapses. In conclusion, high levels of CD271 + Ki-67 + MART1 + cells were linked to

  2. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) Types of records made available. All records of the Committee that are not available under §§ 271.3 and... requester represents an educational or noncommercial scientific institution, or news media; (3) A statement...

  3. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Types of records made available. All records of the Committee that are not available under §§ 271.3 and... requester represents an educational or noncommercial scientific institution, or news media; (3) A statement...

  4. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Requirements for generators of... for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State program must cover all generators covered by 40 CFR part 262. States must require new generators to...

  5. 34 CFR 271.10 - What types of projects may be funded?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... members, and other elementary or secondary school personnel to deal effectively with special educational... 271.10 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND... Secretary awards grants to SEAs for projects offering technical assistance (including training) to school...

  6. 34 CFR 271.10 - What types of projects may be funded?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... members, and other elementary or secondary school personnel to deal effectively with special educational... 271.10 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND... Secretary awards grants to SEAs for projects offering technical assistance (including training) to school...

  7. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  8. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  9. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  10. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  11. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  12. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Francisella tularensis SCHU S4 Infection in BALB/c Mice and Cynomolgus Macaques

    PubMed Central

    Anderson, Michael S.; Christ, David; Gooldy, Melanie; Henning, Lisa N.; Heine, Henry S.; Kindt, M. Victoria; Lin, Winston; Siefkas-Patterson, Kaylyn; Radcliff, Anne K.; Tam, Vincent H.; Sutcliffe, Joyce A.

    2017-01-01

    ABSTRACT TP-271 is a novel, fully synthetic fluorocycline in development for complicated bacterial respiratory infections. TP-271 was active in vitro against a panel of 29 Francisella tularensis isolates, showing MICs against 50% and 90% of isolates of 0.25 and 0.5 μg/ml, respectively. In a mouse model of inhalational tularemia, animals were exposed by aerosol to 91 to 283 50% lethal doses (LD50)/mouse of F. tularensis SCHU S4. Following 21 days of once-daily intraperitoneal dosing with TP-271 at 3, 6, 12, and 18 mg/kg of body weight/day, initiating at 24 h postchallenge, survival was 80%, 100%, 100%, and 100%, respectively. When treatment was initiated at 72 h postchallenge, survival was 89%, 100%, 100%, and 100% in the 3-, 6-, 12-, and 18-mg/kg/day TP-271 groups, respectively. No mice treated with the vehicle control survived. Surviving mice treated with TP-271 showed little to no relapse during 14 days posttreatment. In a nonhuman primate model of inhalational tularemia, cynomolgus macaques received an average aerosol exposure of 1,144 CFU of F. tularensis SCHU S4. Once-daily intravenous infusion with 1 or 3 mg/kg TP-271, or vehicle control, for 21 days was initiated within 6 h of confirmed fever. All animals treated with TP-271 survived to the end of the study, with no relapse during 14 days after the last treatment, whereas no vehicle control-treated animals survived. The protection and low relapse afforded by TP-271 treatment in these studies support continued investigation of TP-271 for use in the event of aerosolized exposure to F. tularensis. PMID:28559261

  13. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Bacillus anthracis Infection in BALB/c Mice and Cynomolgus Macaques.

    PubMed

    Grossman, Trudy H; Anderson, Michael S; Drabek, Lindsay; Gooldy, Melanie; Heine, Henry S; Henning, Lisa N; Lin, Winston; Newman, Joseph V; Nevarez, Rene; Siefkas-Patterson, Kaylyn; Radcliff, Anne K; Sutcliffe, Joyce A

    2017-10-01

    The fluorocycline TP-271 was evaluated in mouse and nonhuman primate (NHP) models of inhalational anthrax. BALB/c mice were exposed by nose-only aerosol to Bacillus anthracis Ames spores at a level of 18 to 88 lethal doses sufficient to kill 50% of exposed individuals (LD 50 ). When 21 days of once-daily dosing was initiated at 24 h postchallenge (the postexposure prophylaxis [PEP] study), the rates of survival for the groups treated with TP-271 at 3, 6, 12, and 18 mg/kg of body weight were 90%, 95%, 95%, and 84%, respectively. When 21 days of dosing was initiated at 48 h postchallenge (the treatment [Tx] study), the rates of survival for the groups treated with TP-271 at 6, 12, and 18 mg/kg TP-271 were 100%, 91%, and 81%, respectively. No deaths of TP-271-treated mice occurred during the 39-day posttreatment observation period. In the NHP model, cynomolgus macaques received an average dose of 197 LD 50 of B. anthracis Ames spore equivalents using a head-only inhalation exposure chamber, and once-daily treatment of 1 mg/kg TP-271 lasting for 14 or 21 days was initiated within 3 h of detection of protective antigen (PA) in the blood. No (0/8) animals in the vehicle control-treated group survived, whereas all 8 infected macaques treated for 21 days and 4 of 6 macaques in the 14-day treatment group survived to the end of the study (56 days postchallenge). All survivors developed toxin-neutralizing and anti-PA IgG antibodies, indicating an immunologic response. On the basis of the results obtained with the mouse and NHP models, TP-271 shows promise as a countermeasure for the treatment of inhalational anthrax. Copyright © 2017 American Society for Microbiology.

  14. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Bacillus anthracis Infection in BALB/c Mice and Cynomolgus Macaques

    PubMed Central

    Anderson, Michael S.; Drabek, Lindsay; Gooldy, Melanie; Heine, Henry S.; Henning, Lisa N.; Lin, Winston; Newman, Joseph V.; Nevarez, Rene; Siefkas-Patterson, Kaylyn; Radcliff, Anne K.; Sutcliffe, Joyce A.

    2017-01-01

    ABSTRACT The fluorocycline TP-271 was evaluated in mouse and nonhuman primate (NHP) models of inhalational anthrax. BALB/c mice were exposed by nose-only aerosol to Bacillus anthracis Ames spores at a level of 18 to 88 lethal doses sufficient to kill 50% of exposed individuals (LD50). When 21 days of once-daily dosing was initiated at 24 h postchallenge (the postexposure prophylaxis [PEP] study), the rates of survival for the groups treated with TP-271 at 3, 6, 12, and 18 mg/kg of body weight were 90%, 95%, 95%, and 84%, respectively. When 21 days of dosing was initiated at 48 h postchallenge (the treatment [Tx] study), the rates of survival for the groups treated with TP-271 at 6, 12, and 18 mg/kg TP-271 were 100%, 91%, and 81%, respectively. No deaths of TP-271-treated mice occurred during the 39-day posttreatment observation period. In the NHP model, cynomolgus macaques received an average dose of 197 LD50 of B. anthracis Ames spore equivalents using a head-only inhalation exposure chamber, and once-daily treatment of 1 mg/kg TP-271 lasting for 14 or 21 days was initiated within 3 h of detection of protective antigen (PA) in the blood. No (0/8) animals in the vehicle control-treated group survived, whereas all 8 infected macaques treated for 21 days and 4 of 6 macaques in the 14-day treatment group survived to the end of the study (56 days postchallenge). All survivors developed toxin-neutralizing and anti-PA IgG antibodies, indicating an immunologic response. On the basis of the results obtained with the mouse and NHP models, TP-271 shows promise as a countermeasure for the treatment of inhalational anthrax. PMID:28784679

  15. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Francisella tularensis SCHU S4 Infection in BALB/c Mice and Cynomolgus Macaques.

    PubMed

    Grossman, Trudy H; Anderson, Michael S; Christ, David; Gooldy, Melanie; Henning, Lisa N; Heine, Henry S; Kindt, M Victoria; Lin, Winston; Siefkas-Patterson, Kaylyn; Radcliff, Anne K; Tam, Vincent H; Sutcliffe, Joyce A

    2017-08-01

    TP-271 is a novel, fully synthetic fluorocycline in development for complicated bacterial respiratory infections. TP-271 was active in vitro against a panel of 29 Francisella tularensis isolates, showing MICs against 50% and 90% of isolates of 0.25 and 0.5 μg/ml, respectively. In a mouse model of inhalational tularemia, animals were exposed by aerosol to 91 to 283 50% lethal doses (LD 50 )/mouse of F. tularensis SCHU S4. Following 21 days of once-daily intraperitoneal dosing with TP-271 at 3, 6, 12, and 18 mg/kg of body weight/day, initiating at 24 h postchallenge, survival was 80%, 100%, 100%, and 100%, respectively. When treatment was initiated at 72 h postchallenge, survival was 89%, 100%, 100%, and 100% in the 3-, 6-, 12-, and 18-mg/kg/day TP-271 groups, respectively. No mice treated with the vehicle control survived. Surviving mice treated with TP-271 showed little to no relapse during 14 days posttreatment. In a nonhuman primate model of inhalational tularemia, cynomolgus macaques received an average aerosol exposure of 1,144 CFU of F. tularensis SCHU S4. Once-daily intravenous infusion with 1 or 3 mg/kg TP-271, or vehicle control, for 21 days was initiated within 6 h of confirmed fever. All animals treated with TP-271 survived to the end of the study, with no relapse during 14 days after the last treatment, whereas no vehicle control-treated animals survived. The protection and low relapse afforded by TP-271 treatment in these studies support continued investigation of TP-271 for use in the event of aerosolized exposure to F. tularensis . Copyright © 2017 American Society for Microbiology.

  16. 26. BUILDING NO. 271I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. BUILDING NO. 271-I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER OF BUILDING SHOWING DOORS TO MIXING ROOM NO. 4. HAND CRANK VISIBLE AT RIGHT ROTATED SMALL POTS (CONTAINING LEAD AZIDE) IN MIXING ROOMS. - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  17. Acceptance test procedure bldg. 271-U remote monitoring of project W-059 B-Plant canyon exhaust system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    MCDANIEL, K.S.

    1999-09-01

    The test procedure provides for verifying indications and alarms The test procedure provides for verifying indications and alarms associated with the B Plant Canyon Ventilation System as they are being displayed on a remote monitoring workstation located in building 271-U. The system application software was installed by PLCS Plus under contract from B&W Hanford Company. The application software was installed on an existing operator workstation in building 271U which is owned and operated by Bechtel Hanford Inc.

  18. 40 CFR 80.271 - How can a small refiner obtain an adjustment of its 2004-2007 per-gallon cap standard?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... adjustment of its 2004-2007 per-gallon cap standard? 80.271 Section 80.271 Protection of Environment...-2007 per-gallon cap standard? (a) EPA may in its discretion adjust the small refiner per-gallon cap... that the per-gallon cap creates; (2) The refiner's proposed adjusted per-gallon cap standard and the...

  19. Aggressive pituitary adenomas occurring in young patients in a large Polynesian kindred with a germline R271W mutation in the AIP gene.

    PubMed

    Jennings, Juliet E; Georgitsi, Marianthi; Holdaway, Ian; Daly, Adrian F; Tichomirowa, Maria; Beckers, Albert; Aaltonen, Lauri A; Karhu, Auli; Cameron, Fergus J

    2009-11-01

    Mutations in the aryl hydrocarbon receptor-interacting protein (AIP) were recently shown to confer a pituitary adenoma predisposition in patients with familial isolated pituitary adenomas (FIPA). We report a large Samoan FIPA kindred from Australia/New Zealand with an R271W mutation that was associated with aggressive pituitary tumors. Case series with germline screening of AIP and haplotype analyses among R271W families. This previously unreported kindred consisted of three affected individuals that either presented with or had first symptoms of a pituitary macroadenoma in late childhood or adolescence. The index case, a 15-year-old male with incipient gigantism and his maternal aunt, had somatotropinomas, and the maternal uncle of the index case had a prolactinoma. All tumors were large (15, 40, and 60 mm maximum diameter) and two required transcranial surgery and radiotherapy. All three affected subjects and ten other unaffected relatives were found to be positive for a germline R271W AIP mutation. Comparison of the single nucleotide polymorphism patterns among this family and two previously reported European FIPA families with the same R271W mutation demonstrated no common ancestry. This kindred exemplifies the aggressive features of pituitary adenomas associated with AIP mutations, while genetic analyses among three R271W FIPA families indicate that R271W represents a mutational hotspot that should be studied further in functional studies.

  20. In vitro migration and proliferation ("wound healing") potential of mesenchymal stromal cells generated from human CD271(+) bone marrow mononuclear cells.

    PubMed

    Latifi-Pupovci, Hatixhe; Kuçi, Zyrafete; Wehner, Sibylle; Bönig, Halvard; Lieberz, Ralf; Klingebiel, Thomas; Bader, Peter; Kuçi, Selim

    2015-09-25

    Emerging evidence indicates that mesenchymal stromal cells (MSCs) isolated from different tissue sources may be used in vivo as tissue restorative agents. To date, there is no evidence, however, on migration and proliferation ("wound healing") potential of different subsets of MSCs. The main goal of this study was therefore to compare the in vitro "wound healing" capacity of MSCs generated from positively selected CD271(+) bone marrow mononuclear cells (CD271-MSCs) and MSCs generated by plastic adherence (PA-MSCs). The in vitro model of wound healing (CytoSelect™ 24-Well Wound Healing Assay) was used in order to compare the migration and proliferation potential of CD271-MSCs and PA-MSCs of passage 2 and 4 cultured in presence or absence of growth factors or cytokines. CD271-MSCs of both passages when compared to PA-MSCs demonstrated a significantly higher potential to close the wound 12 and 24 h after initiation of the wound healing assay (P < 0.003 and P < 0.002, respectively). Noteworthy, the migration capacity of PA-MSCs of second passage was significantly improved after stimulation with FGF-2 (P < 0.02), PDGF-BB (P < 0.006), MCP-1 (P < 0.002) and IL-6 (P < 0.03), whereas only TGF-β enhanced significantly migration process of PA-MSCs of P4 12 h after the treatment (P < 0.02). Interestingly, treatment of CD271-MSCs of both passages with growth factors or cytokines did not affect their migratory potential. Our in vitro data provide the first evidence that CD271-MSCs are significantly more potent in "wound healing" than their counterparts PA-MSCs.

  1. Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fukuchi, Mikoto; Wanotayan, Rujira; Liu, Sicheng

    2015-06-12

    XRCC4 and DNA Ligase IV (LIG4) cooperate to join two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). However, it is not fully understood how these proteins are localized to the nucleus. Here we created XRCC4{sup K271R} mutant, as Lys271 lies within the putative nuclear localization signal (NLS), and XRCC4{sup K210R} mutant, as Lys210 was reported to undergo SUMOylation, implicated in the nuclear localization of XRCC4. Wild-type and mutated XRCC4 with EGFP tag were introduced into HeLa cell, in which endogenous XRCC4 had been knocked down using siRNA directed to 3′-untranslated region,more » and tested for the nuclear localization function by fluorescence microscopy. XRCC4{sup K271R} was defective in the nuclear localization of itself and LIG4, whereas XRCC4{sup K210R} was competent for the nuclear localization with LIG4. To examine DSB repair function, wild-type and mutated XRCC4 were introduced into XRCC4-deficient M10. M10-XRCC4{sup K271R}, but not M10-XRCC4{sup K210R}, showed significantly reduced surviving fraction after 2 Gy γ-ray irradiation as compared to M10-XRCC4{sup WT}. The number of γ-H2AX foci remaining 2 h after 2 Gy γ-ray irradiation was significantly greater in M10-XRCC4{sup K271R} than in M10-XRCC4{sup WT}, whereas it was only marginally increased in M10-XRCC4{sup K210R} as compared to M10-XRCC4{sup WT}. The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4. - Highlights: • XRCC4{sup K271R} is defective in the nuclear localization of itself and LIG4. • XRCC4{sup K210R} is competent for the nuclear localization of itself and LIG4. • XRCC4{sup K271R} is deficient in DSB repair function. • XRCC4{sup K210R} is mostly normal in DSB repair function.« less

  2. 27. BUILDING NO. 271I, INTERIOR OF LEAD AZIDE MIXING ROOM. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. BUILDING NO. 271-I, INTERIOR OF LEAD AZIDE MIXING ROOM. HERE, TWO POUNDS (MAXIMUM) OF LEAD AZIDE WAS MIXED IN SEVEN SMALL POTS EACH SEPARATED FROM THE OTHERS BY THE STEEL WALLS PICTURED HERE. THE POTS ARE ALL FIXED TO A COMMON SHAFT WHICH IS ROTATED BY A HAND CRANK OUTSIDE OF ROOM (SEE HAER NJ-36A-26). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  3. Photodissociation dynamics of nitromethane at 226 and 271 nm at both nanosecond and femtosecond time scales.

    PubMed

    Guo, Y Q; Bhattacharya, A; Bernstein, E R

    2009-01-08

    Photodissociation of nitromethane has been investigated for decades both theoretically and experimentally; however, as a whole picture, the dissociation dynamics for nitromethane are still not clear, although many different mechanisms have been proposed. To make a complete interpretation of these different mechanisms, photolysis of nitromethane at 226 and 271 nm under both collisional and collisionless conditions is investigated at nanosecond and femtosecond time scales. These two laser wavelengths correspond to the pi* <-- pi and pi* <-- n excitations of nitromethane, respectively. In nanosecond 226 nm (pi* <-- pi) photolysis experiments, CH(3) and NO radicals are observed as major products employing resonance enhanced multiphoton ionization techniques and time-of-flight mass spectrometry. Additionally, OH and CH(3)O radicals are weakly observed as dissociation products employing laser induced fluorescence spectroscopy; the CH(3)O product is only observed under collisional conditions. In femtosecond 226 nm experiments, CH(3), NO(2), and NO products are observed. These results confirm that rupture of C-N bond should be the main primary process for the photolysis of nitromethane after the pi* <-- pi excitation at 226 nm, and the NO(2) molecule should be the precursor of the observed NO product. Formation of the CH(3)O radical after the recombination of CH(3) and NO(2) species under collisional conditions rules out a nitro-nitrite isomerization mechanism for the generation of CH(3)O and NO from pi pi* CH(3)NO(2). The OH radical formation for pi pi* CH(3)NO(2) should be a minor dissociation channel because of the weak OH signal in both nanosecond and femtosecond (nonobservable) experiments. Single color femtosecond pump-probe experiments at 226 nm are also employed to monitor the dynamics of the dissociation of nitromethane after the pi* <-- pi excitation. Because of the ultrafast dynamics of product formation at 226 nm, the pump-probe transients for the three

  4. Corrective Action Plan for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems, Nevada Test Site, Nevada

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    R. B. Jackson

    2003-05-01

    The Areas 25, 26 and 27 Septic Systems are in the Federal Facility Agreement and Consent Order (FFACO) of 1996 as Corrective Action Unit (CAU) 271. This Corrective Action Plan (CAP) provides selected corrective action alternatives and proposes the closure methodology for CAU 271. CAU 271 is located on the Nevada Test Site (NTS) approximately 105 kilometers (65 miles) northwest of Las Vegas, Nevada, and consists of the following 15 Corrective Action Sites (CAS): CAS 25-04-1, Septic System; CAS 25-04-03, Septic System; CAS25-04-04, Septic System; CAS 25-04-08, Septic System; CAS 25-04-09, Septic System; CAS 25-04-10, Septic System; CAS 25-04-11, Septicmore » System; CAS 26-03-01, Contaminated Water Reservoir; CAS 26-04-1, Septic System; CAS 26-04-02, Septic System; CAS 26-05-01, Radioactive Leachfield; CAS-26-05-03, Septic System; CAS 26-05-04, Septic System; CAS 26-05-05, Septic System; and CAS 27-05-02, Leachfield.« less

  5. 30 CFR 250.271 - For what reasons will MMS disapprove the DPP or DOCD?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Decision Process for the Dpp Or Docd § 250.271 For what reasons will MMS disapprove the DPP or DOCD? The...), implementing regulations, or other applicable Federal laws. (b) No consistency concurrence. (1) An affected State has not yet issued a final decision on your coastal zone consistency certification (see 15 CFR 930...

  6. Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum-plasma membrane contact sites.

    PubMed

    Siao, Wei; Wang, Pengwei; Voigt, Boris; Hussey, Patrick J; Baluska, Frantisek

    2016-11-01

    Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulum-plasma membrane (ER-PM) contact sites in leaf and root cells. The ER-PM localization of Arabidopsis SYT1 resembles that of the extended synaptotagmins (E-SYTs) in animal cells. In mammals, E-SYTs have been shown to regulate calcium signaling, lipid transfer, and endocytosis. Arabidopsis SYT1 was reported to be essential for maintaining cell integrity and virus movement. This study provides detailed insight into the subcellular localization of SYT1 and VAP27-1, another ER-PM-tethering protein. SYT1 and VAP27-1 were shown to be localized on distinct ER-PM contact sites. The VAP27-1-enriched ER-PM contact sites (V-EPCSs) were always in contact with the SYT1-enriched ER-PM contact sites (S-EPCSs). The V-EPCSs still existed in the leaf epidermal cells of the SYT1 null mutant; however, they were less stable than those in the wild type. The polygonal networks of cortical ER disassembled and the mobility of VAP27-1 protein on the ER-PM contact sites increased in leaf cells of the SYT1 null mutant. These results suggest that SYT1 is responsible for stabilizing the ER network and V-EPCSs. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  7. Rates of upgrade to malignancy for 271 cases of flat epithelial atypia (FEA) diagnosed by breast core biopsy.

    PubMed

    Peres, Alexandre; Barranger, Emmanuel; Becette, Véronique; Boudinet, Alain; Guinebretiere, Jean-Marc; Cherel, Pascal

    2012-06-01

    Flat epithelial atypia (FEA) is a borderline lesion that might represent an early stage in the development of certain low-grade carcinomas in situ and invasive cancers. There are no guidelines on its management. Our objectives were to determine the upgrade to malignancy rate and identify a subpopulation of patients that might undergo just mammographic surveillance. We retrospectively reviewed the data for 271 FEA cases among 5,555 breast core biopsies obtained over a 7-year period (January 2003-2010). We collated clinical data (age, history of cancer, menopausal status), radiological data (lesion type, size, Bi-Rads category), technical data (number of biopsies, needle gauge, excision quality) and histological data and sought correlations between these factors and upgrade rate. The 271 FEA comprised 128 cases of pure FEA, 135 cases of FEA + atypical ductal hyperplasia, and 8 cases of FEA + atypical lobular hyperplasia. Overall, 184 patients underwent surgery and 46 mammographic surveillance. Surgery detected 34 cases of malignancy (23 CIS, 7 invasive cancers, and 4 mixed cases) giving a 15% upgrade rate. Quality of excision was the only factor associated with under-diagnosis. The presence of FEA at biopsy warrants surgery.

  8. Hyperekplexia mutation R271L of alpha1 glycine receptors potentiates allosteric interactions of nortropeines, propofol and glycine with [3H]strychnine binding.

    PubMed

    Maksay, Gábor; Bíró, Tímea; Laube, Bodo; Nemes, Péter

    2008-01-01

    Human alpha1 and hyperekplexia mutant alpha1(R271L) glycine receptors (GlyRs) were transiently expressed in human embryonic kidney 293 cells for [3H]strychnine binding. Binding parameters were determined using a ternary allosteric model. The hyperekplexia mutation increased the positive cooperativity of 0.3 mM propofol and glycine binding by about six times: the cooperativity factor beta was 0.26 for alpha1 GlyRs and 0.04 for alpha1(R271L) GlyRs. Thus, propofol restored the potency of glycine impaired by the mutation. Five nortropeines, i.e. substituted benzoates of nortropine and a new compound, nortropisetron were prepared and also examined on [3H]strychnine binding. They showed nanomolar displacing potencies amplified by the hyperekplexia mutation. The affinity of nor-O-zatosetron (2.6 nM) is one of the highest reported for GlyRs. This binding test offers an in vitro method to evaluate agents against neurological disorders associated with inherited mutations of GlyRs.

  9. Three-dimensional structure of phosphoribosyl pyrophosphate synthetase from E. coli at 2.71 Å resolution

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Timofeev, V. I., E-mail: inna@ns.crys.ras.ru, E-mail: tostars@mail.ru, E-mail: ugama@yandex.ru; Abramchik, Yu. A.; Zhukhlistova, N. E.

    2016-01-15

    Phosphoribosyl pyrophosphate synthetase from Escherichia coli was cloned, purified, and crystallized. Single crystals of the enzyme were grown under microgravity. The X-ray diffraction data set was collected at the Spring-8 synchrotron facility and used to determine the three-dimensional structure of the enzyme by the molecular-replacement method at 2.71 Å resolution. The active and regulatory sites in the molecule of E. coli phosphoribosyl pyrophosphate synthetase were revealed by comparison with the homologous protein from Bacillus subtilis, the structure of which was determined in a complex with functional ligands. The conformations of polypeptide-chain fragments surrounding and composing the active and regulatory sitesmore » were shown to be identical in both proteins.« less

  10. Opportunities for increasing natural gas production in the near term. Volume VI. The East Cameron Block 271 Field. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1978-02-01

    This report examines the potential for increasing the rate of production of natural gas from the East Cameron Block 271 Field in the Gulf of Mexico Outer Continental Shelf. Proved reserves are estimated using all available reservoir data, including well logs and pressure tests, and cost parameters typical in the area. Alternative schedules for future production are devised, and net present values calculated from which the maximum production rate that also maximizes net present value is determined.

  11. [A study on genotype of 271 mycobacterium tuberculosis isolates in 6 prefectures in Yunnan Province].

    PubMed

    Chen, L Y; Yang, X; Ru, H H; Yang, H J; Yan, S Q; Ma, L; Chen, J O; Yang, R; Xu, L

    2018-01-06

    Objective: To understand the characteristics of genotypes of Mycobacterium tuberculosis isolates in Yunnan province, and provide the molecular epidemiological evidence for prevention and control of tuberculosis in Yunnan Province. Methods: Mycobacterium Tuberculosis isolates were collected from 6 prefectures of Yunnan province in 2014 and their Genetypes of Mycobacterium tuberculosis isolates were obtained using spoligotyping and multiple locus variable numbers of tandem repeats analysis (MLVA). The results of spoligotyping were entered into the SITVITWEB database to obtain the Spoligotyping International Type (SIT) patterns and the sublineages of MTB isolates. The genoyping patterns were clustered with BioNumerics (version 5.0). Results: A total of 271 MTB isolates represented patients were collected from six prefectures in Yunnan province. Out of these patients, 196 (72.3%) were male. The mean age of the patients was (41.9±15.1) years. The most MTB isolates were from Puer, totally 94 iusolates(34.69%). Spoligotyping analysis revealed that 151 (55.72%) MTB isolates belonged to the Beijing genotype, while the other 120 (44.28%) were from non-Beijing genotype; 40 genotypes were consisted of 24 unique genotypes and 16 clusters. The 271 isolates were differentiated into 30 clusters (2 to 17 isolates per cluster) and 177 unique genotypes, showing a clustering rate of 23.62%. Beijing genotype strains showed higher clustering rate than non-Beijing genotype strains (29.14% vs 16.67%). The HGI of 12-locus VNTR in total MTB strains, Beijing genotype strains and non-Beijing genotype was 0.993, 0.982 and 0.995 respectively. Conclusion: The Beijing genotype was the predominant genotype in Yunnan Province, the characteristics of Mycobacterium tuberculosis showed high genetic diversity. The genotyping data reflect the potential recent ongoing transmission in some area, which highlights the urgent need for early diagnosis and treatment of the infectious TB cases, to cut off the

  12. Plant Endocytosis Requires the ER Membrane-Anchored Proteins VAP27-1 and VAP27-3.

    PubMed

    Stefano, Giovanni; Renna, Luciana; Wormsbaecher, Clarissa; Gamble, Jessie; Zienkiewicz, Krzysztof; Brandizzi, Federica

    2018-05-22

    Through yet-undefined mechanisms, the plant endoplasmic reticulum (ER) has a critical role in endocytosis. The plant ER establishes a close association with endosomes and contacts the plasma membrane (PM) at ER-PM contact sites (EPCSs) demarcated by the ER membrane-associated VAMP-associated-proteins (VAP). Here, we investigated two plant VAPs, VAP27-1 and VAP27-3, and found an interaction with clathrin and a requirement for the homeostasis of clathrin dynamics at endocytic membranes and endocytosis. We also demonstrated direct interaction of VAP27-proteins with phosphatidylinositol-phosphate lipids (PIPs) that populate endocytic membranes. These results support that, through interaction with PIPs, VAP27-proteins bridge the ER with endocytic membranes and maintain endocytic traffic, likely through their interaction with clathrin. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

  13. Identification of tetrapeptides from a mixture based positional scanning library that can restore nM full agonist function of the L106P, I69T, I102S, A219V, C271Y, and C271R human melanocortin-4 polymorphic receptors (hMC4Rs).

    PubMed

    Haslach, Erica M; Huang, Huisuo; Dirain, Marvin; Debevec, Ginamarie; Geer, Phaedra; Santos, Radleigh G; Giulianotti, Marc A; Pinilla, Clemencia; Appel, Jon R; Doering, Skye R; Walters, Michael A; Houghten, Richard A; Haskell-Luevano, Carrie

    2014-06-12

    Human obesity has been linked to genetic factors and single nucleotide polymorphisms (SNPs). Melanocortin-4 receptor (MC4R) SNPs have been associated with up to 6% frequency in morbidly obese children and adults. A potential therapy for individuals possessing such genetic modifications is the identification of molecules that can restore proper receptor signaling and function. These compounds could serve as personalized medications improving quality of life issues as well as alleviating diseases symptoms associated with obesity including type 2 diabetes. Several hMC4 SNP receptors have been pharmacologically characterized in vitro to have a decreased, or a lack of response, to endogenous agonists such as α-, β-, and γ2-melanocyte stimulating hormones (MSH) and adrenocorticotropin hormone (ACTH). Herein we report the use of a mixture based positional scanning combinatorial tetrapeptide library to discover molecules with nM full agonist potency and efficacy to the L106P, I69T, I102S, A219V, C271Y, and C271R hMC4Rs. The most potent compounds at all these hMC4R SNPs include Ac-His-(pI)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Arg-(pI)Phe-NH2, and Ac-Arg-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, revealing new ligand pharmacophore models for melanocortin receptor drug design strategies.

  14. Identification of Tetrapeptides from a Mixture Based Positional Scanning Library That Can Restore nM Full Agonist Function of the L106P, I69T, I102S, A219V, C271Y, and C271R Human Melanocortin-4 Polymorphic Receptors (hMC4Rs)

    PubMed Central

    2015-01-01

    Human obesity has been linked to genetic factors and single nucleotide polymorphisms (SNPs). Melanocortin-4 receptor (MC4R) SNPs have been associated with up to 6% frequency in morbidly obese children and adults. A potential therapy for individuals possessing such genetic modifications is the identification of molecules that can restore proper receptor signaling and function. These compounds could serve as personalized medications improving quality of life issues as well as alleviating diseases symptoms associated with obesity including type 2 diabetes. Several hMC4 SNP receptors have been pharmacologically characterized in vitro to have a decreased, or a lack of response, to endogenous agonists such as α-, β-, and γ2-melanocyte stimulating hormones (MSH) and adrenocorticotropin hormone (ACTH). Herein we report the use of a mixture based positional scanning combinatorial tetrapeptide library to discover molecules with nM full agonist potency and efficacy to the L106P, I69T, I102S, A219V, C271Y, and C271R hMC4Rs. The most potent compounds at all these hMC4R SNPs include Ac-His-(pI)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Arg-(pI)Phe-NH2, and Ac-Arg-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, revealing new ligand pharmacophore models for melanocortin receptor drug design strategies. PMID:24517312

  15. From COST 271 to 296 EU actions on ionospheric monitoring and modelling for terrestrial and Earth space radio systems

    NASA Astrophysics Data System (ADS)

    Zolesi, B.; Cander, Lj. R.; Altadill, D.

    The ionospheric community has long been aware that co-operative research on an international basis is essential to deal with temporal and spatial changes in the ionosphere that influence the performance of terrestrial and Earth-space radio systems. The EU COST (Co-operation in the field of Scientific and Technical Research) 271 Action on "Effects of the Upper Atmosphere on Terrestrial and Earth-space Communications" has had during the period of October 2000-August 2004 the following main objectives: (1) to evaluate the influence of upper atmospheric conditions on terrestrial and Earth-space communications, (2) to develop methods and techniques to improve ionospheric models over Europe for telecommunication and navigation applications and (3) to transfer the results to the appropriate radiocommunication study groups of the International Telecommunication Union (ITU-R) and other national and international organizations dealing with the modern communication systems. At the beginning of 2005 the new 296 Action in the COST Telecommunications, Information Science and Technology domain on "Mitigation of Ionospheric Effects on Radio Systems (MIERS)" was approved for the period 2005-2009. The main objectives of the MIERS are: (a) to support and enhanced the existing European facilities for historical and real-time digital ionospheric data collection and exchange; (b) to develop an integrated approach to ionospheric modelling, create the mechanism needed to ingest processed data into models, extend and develop suitable mitigation models and define the protocols needed to link models together; and (c) to strengthen the areas of expertise that already exist by stimulating closer cooperation between scientists and users, focusing the scope of all the previous COST ionospheric related studies to the mitigation of ionospheric effects on radio systems. This paper summarises briefly how the major objectives of the COST271 Action have been achieved and what are the most important

  16. In Vitro Activities of Gemifloxacin versus Five Quinolones and Two Macrolides against 271 Spanish Isolates of Legionella pneumophila: Influence of Charcoal on Susceptibility Test Results

    PubMed Central

    García, M. T.; Pelaz, C.; Giménez, M. J.; Aguilar, L.

    2000-01-01

    The MICs at which 90% of isolates are inhibited for gemifloxacin, trovafloxacin, and grepafloxacin were low (≤0.01 μg/ml) for 271 Legionella isolates when they were determined by the broth microdilution method but increased (≥6 dilutions) when they were determined by the agar dilution method. This was due to the charcoal in the agar dilution medium, as shown by the progressive decrease in the MICs when the charcoal concentrations decreased. As free drug is the active fraction, charcoal binding should be considered. PMID:10898695

  17. The central star candidate of the planetary nebula Sh2-71: photometric and spectroscopic variability

    NASA Astrophysics Data System (ADS)

    Močnik, T.; Lloyd, M.; Pollacco, D.; Street, R. A.

    2015-07-01

    We present the analysis of several newly obtained and archived photometric and spectroscopic data sets of the intriguing and yet poorly understood 13.5 mag central star candidate of the bipolar planetary nebula Sh2-71. Photometric observations confirmed the previously determined quasi-sinusoidal light curve with a period of 68 d and also indicated periodic sharp brightness dips, possibly eclipses, with a period of 17.2 d. In addition, the comparison between U and V light curves revealed that the 68 d brightness variations are accompanied by a variable reddening effect of ΔE(U - V) = 0.38. Spectroscopic data sets demonstrated pronounced variations in spectral profiles of Balmer, helium and singly ionized metal lines and indicated that these variations occur on a time-scale of a few days. The most accurate verification to date revealed that spectral variability is not correlated with the 68 d brightness variations. The mean radial velocity of the observed star was measured to be ˜26 km s-1 with an amplitude of ±40 km s-1. The spectral type was determined to be B8V through spectral comparison with synthetic and standard spectra. The newly proposed model for the central star candidate is a Be binary with a misaligned precessing disc.

  18. Design of 9.271-pressure-ratio 5-stage core compressor and overall performance for first 3 stages

    NASA Technical Reports Server (NTRS)

    Steinke, Ronald J.

    1986-01-01

    Overall aerodynamic design information is given for all five stages of an axial flow core compressor (74A) having a 9.271 pressure ratio and 29.710 kg/sec flow. For the inlet stage group (first three stages), detailed blade element design information and experimental overall performance are given. At rotor 1 inlet tip speed was 430.291 m/sec, and hub to tip radius ratio was 0.488. A low number of blades per row was achieved by the use of low-aspect-ratio blading of moderate solidity. The high reaction stages have about equal energy addition. Radial energy varied to give constant total pressure at the rotor exit. The blade element profile and shock losses and the incidence and deviation angles were based on relevant experimental data. Blade shapes are mostly double circular arc. Analysis by a three-dimensional Euler code verified the experimentally measured high flow at design speed and IGV-stator setting angles. An optimization code gave an optimal IGV-stator reset schedule for higher measured efficiency at all speeds.

  19. Comparison of referral and non-referral hypertensive disorders during pregnancy: an analysis of 271 consecutive cases at a tertiary hospital.

    PubMed

    Liu, Ching-Ming; Chang, Shuenn-Dyh; Cheng, Po-Jen

    2005-05-01

    This retrospective cohort study analyzed the clinical manifestations in patients with preeclampsia and eclampsia, assessed the risk factors compared to the severity of hypertensive disorders on maternal and perinatal morbidity, and mortality between the referral and non-referral patients. 271 pregnant women with preeclampsia and eclampsia were assessed (1993 to 1997). Chi-square analysis was used for the comparison of categorical variables, and the comparison of the two independent variables of proportions in estimation of confidence intervals and calculated odds ratio of the referral and non-referral groups. Multivariate logistic regression was used for adjusting potential confounding risk factors. Of the 271 patients included in this study, 71 (26.2%) patients were referrals from other hospitals. Most of the 62 (87.3%) referral patients were transferred during the period 21 and 37 weeks of gestation. Univariate analysis revealed that referral patients with hypertensive disorder were significantly associated with SBP > or =180, DBP > or =105, severe preclampsia, haemolysis, elevated liver enzymes, low platelets (HELLP), emergency C/S, maternal complications, and low birth weight babies, as well as poor Apgar score. Multivariate logistic regression analyses revealed that the risk factors identified to be significantly associated with increased risk of referral patients included: diastolic blood pressure above 105 mmHg (adjusted odds ratio, 2.09; 95 percent confidence interval, 1.06 to 4.13; P = 0.034), severe preeclampsia (adjusted odds ratio, 3.46; 95 percent confidence interval, 1.76 to 6.81; P < 0.001), eclampsia (adjusted odds ratio, 2.77; 95 percent confidence interval, 0.92 to 8.35; P = 0.071), HELLP syndrome (adjusted odds ratio, 18.81; 95 percent confidence interval, 2.14 to 164.99; P = 0.008). The significant factors associated with the referral patients with hypertensive disorders were severe preeclampsia, HELLP, and eclampsia. Lack of prenatal care was

  20. Characterization of endo-β-mannanase from Enterobacter ludwigii MY271 and application in pulp industry.

    PubMed

    Yang, Miao; Cai, Jun; Wang, Changgao; Du, Xin; Lin, Jianguo

    2017-01-01

    β-Mannanases are the second most important enzymes for the hydrolysis of hemicelluloses. An endo-β-mannanase from Enterobacter ludwigii MY271 was purified at 11.7 ± 0.2-fold to homogeneity with a final recovery of 15.2 ± 0.2 %. Using purified β-mannanase protein and SDS-PAGE, the molecular mass was found to be 43.16 kDa. The optimal pH and temperature of the enzyme was found to be 7.0 and 55 °C, respectively. The β-mannanase activity was stable over a broad pH range of pH 2.0-10.0. In addition, the purified enzyme was highly activated by several metal ions and chemical reagents, such as Mg 2+ , L-cysteine, glutathione (GSH) and β-mercaptoethanol. Whereas the enzyme was strongly inhibited by Hg 2+ , Cu 2+ , N-bromosuccinimide (NBS), 1-ethyl-3-(3-dimethyl-amino-propyl)-carbodiimide (EDC), phenylmethanesulfonyl fluoride (PMSF), and sodium dodecyl sulfate (SDS). The β-mannanase was highly active towards glucomannan, and showed endo-activity by producing a mixture of oligosaccharides. Moreover, the enzyme displayed a classical endo-type mode on mannooligosaccharides. The β-mannanase coupled with xylanase significantly improved the brightness of kraft pulp, whereas it has no remarkable effect on the tensile strength of the pulp. Our functional studies of the purified β-mannanase indicate that the enzyme is beneficial to industrial applications, in particular, biotechnological processes, such as food, feed and pulp industry.

  1. Performance Characterization of Loctite (Registered Trademark) 242 and 271 Liquid Locking Compounds (LLCs) as a Secondary Locking Feature for International Space Station (ISS) Fasteners

    NASA Technical Reports Server (NTRS)

    Dube, Michael J.; Gamwell, Wayne R.

    2011-01-01

    Several International Space Station (ISS) hardware components use Loctite (and other polymer based liquid locking compounds (LLCs)) as a means of meeting the secondary (redundant) locking feature requirement for fasteners. The primary locking method is the fastener preload, with the application of the Loctite compound which when cured is intended to resist preload reduction. The reliability of these compounds has been questioned due to a number of failures during ground testing. The ISS Program Manager requested the NASA Engineering and Safety Center (NESC) to characterize and quantify sensitivities of Loctite being used as a secondary locking feature. The findings and recommendations provided in this investigation apply to the anaerobic LLCs Loctite 242 and 271. No other anaerobic LLCs were evaluated for this investigation. This document contains the findings and recommendations of the NESC investigation

  2. The Unusual Central Star of the Planetary Nebula Sh 2-71

    NASA Astrophysics Data System (ADS)

    Močnik, Teo

    2013-08-01

    This thesis presents new photometric and spectroscopic observational results for the unusual central star of bipolar planetary nebula Sh2-71. The combined lightcurve, composed from the photometric datasets of three different telescopes, was in agreement with the reported ephemeris of the sinusoidal brightness variations with a period of 68 days. The two sharp brightness dips, indicated by the preliminary automated data reduction process, were confirmed. The presence of three additional dips tentatively suggested that the dips, possibly eclipses, are occurring periodically with a period of 17.2 days. The comparison between U and V lightcurves revealed that the 68 day brightness variations are accompanied by a variable reddening effect. Spectroscopic observations revealed pronounced spectral variations, which were not correlated with the 68 days brightness phase. On the other hand, the high-cadence echelle spectra did not exhibit any variability on hourly timescales, which implied that the spectral variations must occur on timescales of a few days. Radial velocity measurements suggested an amplitude of ±40 km/s but were not correlated with the brightness phase. The measured average radial velocity of the observed star 26 km/s was in near agreement with the reported mean radial velocity of the planetary nebula. As some doubt has been raised recently that the central star could be another field star, this near agreement between the radial velocities provided supporting evidence that the observed star actually is the central star of the planetary nebula. The comparison between the measured and synthetic spectra yielded stellar atmospheric parameters T_eff 12000 K, log(g) 4.0 cm/s^2, vrot\\cdot sin(i) 200 km/s with an indicated high value of metallicity. Fitted stellar parameters and the comparison with standard spectra classified the star as B8V. The obtained spectrophotometric observations have been used to construct a model for the central star. A previously

  3. High-Throughput Analytical Techniques for Determination of Residues of 653 Multiclass Pesticides and Chemical Pollutants in Tea, Part VI: Study of the Degradation of 271 Pesticide Residues in Aged Oolong Tea by Gas Chromatography-Tandem Mass Spectrometry and Its Application in Predicting the Residue Concentrations of Target Pesticides.

    PubMed

    Chang, Qiao-Ying; Pang, Guo-Fang; Fan, Chun-Lin; Chen, Hui; Wang, Zhi-Bin

    2016-07-01

    The degradation rate of 271 pesticide residues in aged Oolong tea at two spray concentrations, named a and b (a < b), were monitored for 120 days using GC-tandem MS (GC-MS/MS). To research the degradation trends and establish regression equations, determination days were plotted as horizontal ordinates and the residue concentrations of pesticide were plotted as vertical ordinates. Here, we consider the degradation equations of 271 pesticides over 40 and 120 days, summarize the degradation rates in six aspects (A-F), and discuss the degradation trends of the 271 pesticides in aged Oolong tea in detail. The results indicate that >70% of the determined pesticides coincide with the degradation regularity of trends A, B, and E, i.e., the concentration of pesticide will decrease within 4 months. Next, 20 representative pesticides were selected for further study at higher spray concentrations, named c and d (d > c > b > a), in aged Oolong tea over another 90 days. The determination days were plotted on the x-axis, and the differences between each determined result and first-time-determined value of target pesticides were plotted on the y-axis. The logarithmic function was obtained by fitting the 90-day determination results, allowing the degradation value of a target pesticide on a specific day to be calculated. These logarithmic functions at d concentration were applied to predict the residue concentrations of pesticides at c concentration. Results revealed that 70% of the 20 pesticides had the lower deviation ratios of predicted and measured results.

  4. Weight outcomes audit for 34,271 adults referred to a primary care/commercial weight management partnership scheme.

    PubMed

    Stubbs, R James; Pallister, Carolyn; Whybrow, Stephen; Avery, Amanda; Lavin, Jacquie

    2011-01-01

    This project audited rate and extent of weight loss in a primary care/commercial weight management organisation partnership scheme. 34,271 patients were referred to Slimming World for 12 weekly sessions. Data were analysed using individual weekly weight records. Average (SD) BMI change was -1.5 kg/m² (1.3), weight change -4.0 kg (3.7), percent weight change -4.0% (3.6), rate of weight change -0.3 kg/week, and number of sessions attended 8.9 (3.6) of 12. For patients attending at least 10 of 12 sessions (n = 19,907 or 58.1%), average (SD) BMI change was -2.0 kg/m² (1.3), weight change -5.5 kg (3.8), percent weight change -5.5% (3.5), rate of weight change -0.4 kg/week, and average number of sessions attended was 11.5 (0.7) (p < 0.001, compared to all patients). Weight loss was greater in men (n = 3,651) than in women (n = 30,620) (p < 0.001). 35.8% of all patients enrolled and 54.7% in patients attending 10 or more sessions achieved at least 5% weight loss. Weight gain was prevented in 92.1% of all patients referred. Attendance explained 29.6% and percent weight lost in week 1 explained 18.4% of the variance in weight loss. Referral to a commercial organisation is a practical option for National Health Service (NHS) weight management strategies, which achieves clinically safe and effective weight loss. Copyright © 2011 S. Karger AG, Basel.

  5. The Role of Neurotrophin Signaling in Gliomagenesis: A Focus on the p75 Neurotrophin Receptor (p75NTR/CD271).

    PubMed

    Alshehri, M M; Robbins, S M; Senger, D L

    2017-01-01

    The p75 neurotrophin receptor (p75 NTR , a.k.a. CD271), a transmembrane glycoprotein and a member of the tumor necrosis family (TNF) of receptors, was originally identified as a nerve growth factor receptor in the mid-1980s. While p75 NTR is recognized to have important roles during neural development, its presence in both neural and nonneural tissues clearly supports the potential to mediate a broad range of functions depending on cellular context. Using an unbiased in vivo selection paradigm for genes underlying the invasive behavior of glioma, a critical characteristic that contributes to poor clinical outcome for glioma patients, we identified p75 NTR as a central regulator of glioma invasion. Herein we review the expanding role that p75 NTR plays in glioma progression with an emphasis on how p75 NTR may contribute to the treatment refractory nature of glioma. Based on the observation that p75 NTR is expressed and functional in two critical glioma disease reservoirs, namely, the highly infiltrative cells that evade surgical resection, and the radiation- and chemotherapy-resistant brain tumor-initiating cells (also referred to as brain tumor stem cells), we propose that p75 NTR and its myriad of downstream signaling effectors represent rationale therapeutic targets for this devastating disease. Lastly, we provide the provocative hypothesis that, in addition to the well-documented cell autonomous signaling functions, the neurotrophins, and their respective receptors, contribute in a cell nonautonomous manner to drive the complex cellular and molecular composition of the brain tumor microenvironment, an environment that fuels tumorigenesis. © 2017 Elsevier Inc. All rights reserved.

  6. Long-term posttraumatic stress symptoms among 3,271 civilian survivors of the September 11, 2001, terrorist attacks on the World Trade Center.

    PubMed

    DiGrande, Laura; Neria, Yuval; Brackbill, Robert M; Pulliam, Paul; Galea, Sandro

    2011-02-01

    Although the September 11, 2001, terrorist attacks were the largest human-made disaster in US history, there is little extant research documenting the attacks' consequences among those most directly affected, that is, persons who were in the World Trade Center towers. Data from a cross-sectional survey conducted 2-3 years after the attacks ascertained the prevalence of long-term, disaster-related posttraumatic stress symptoms and probable posttraumatic stress disorder (PTSD) in 3,271 civilians who evacuated World Trade Center towers 1 and 2. Overall, 95.6% of survivors reported at least 1 current posttraumatic stress symptom. The authors estimated the probable rate of PTSD at 15.0% by using the PTSD Checklist. Women and minorities were at an increased risk of PTSD. A strong inverse relation with annual income was observed. Five characteristics of direct exposure to the terrorist attacks independently predicted PTSD: being on a high floor in the towers, initiating evacuation late, being caught in the dust cloud that resulted from the tower collapses, personally witnessing horror, and sustaining an injury. Working for an employer that sustained fatalities also increased risk. Each addition of an experience of direct exposure resulted in a 2-fold increase in the risk of PTSD (odds ratio = 2.09, 95% confidence interval: 1.84, 2.36). Identification of these risk factors may be useful when screening survivors of large-scale terrorist events for long-term psychological sequelae.

  7. 38 CFR 17.271 - Eligibility.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... date of the onset of the disability; or (iii) The twenty-third birthday of the child, whichever occurs first. (Authority: 38 U.S.C. 501, 1781) (b) CHAMPVA and Medicare entitlement. (1) Individuals under age...) Individuals age 65 or older, and not entitled to Medicare Part A, retain CHAMPVA eligibility. Note to...

  8. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... alone, or in conjunction with another access device, to obtain payments, allotments, benefits, money... retail food business operated from a truck, bus, pushcart, or other mobile vehicle. Identification (ID...

  9. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... alone, or in conjunction with another access device, to obtain payments, allotments, benefits, money... retail food business operated from a truck, bus, pushcart, or other mobile vehicle. Identification (ID...

  10. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... alone, or in conjunction with another access device, to obtain payments, allotments, benefits, money... retail food business operated from a truck, bus, pushcart, or other mobile vehicle. Identification (ID...

  11. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... to an amendment published at 75 FR 78153, Dec. 15, 2010. Access device means any card, plate, code... evaluation of employability skills coupled with counseling on how and where to search for employment. If combined with work experience, employment search or training, an assessment of this nature could constitute...

  12. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... this section) and which is operated solely for the purpose of conducting scientific research, the... program of scholarly research. (f) Federal Reserve Bank means one of the district Banks authorized by the... performance of duties for, or pursuant to the direction of, the Committee. (h) Noncommercial scientific...

  13. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... this section) and which is operated solely for the purpose of conducting scientific research, the... program of scholarly research. (f) Federal Reserve Bank means one of the district Banks authorized by the... performance of duties for, or pursuant to the direction of, the Committee. (h) Noncommercial scientific...

  14. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... this section) and which is operated solely for the purpose of conducting scientific research, the... program of scholarly research. (f) Federal Reserve Bank means one of the district Banks authorized by the... performance of duties for, or pursuant to the direction of, the Committee. (h) Noncommercial scientific...

  15. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... this section) and which is operated solely for the purpose of conducting scientific research, the... program of scholarly research. (f) Federal Reserve Bank means one of the district Banks authorized by the... performance of duties for, or pursuant to the direction of, the Committee. (h) Noncommercial scientific...

  16. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  17. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  18. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  19. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  20. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  1. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... benefits are issued directly to the household, without the use of an intermediary document, based on the... the eligibility to receive the benefits is based upon the disability or blindness criteria used under... is determined to be disabled based upon the criteria used under title XVI of the Social Security Act...

  2. Characterization of novel biomarkers in selecting for subtype specific medulloblastoma phenotypes.

    PubMed

    Liang, Lisa; Aiken, Christopher; McClelland, Robyn; Morrison, Ludivine Coudière; Tatari, Nazanin; Remke, Marc; Ramaswamy, Vijay; Issaivanan, Magimairajan; Ryken, Timothy; Del Bigio, Marc R; Taylor, Michael D; Werbowetski-Ogilvie, Tamra E

    2015-11-17

    Major research efforts have focused on defining cell surface marker profiles for characterization and selection of brain tumor stem/progenitor cells. Medulloblastoma is the most common primary malignant pediatric brain cancer and consists of 4 molecular subgroups: WNT, SHH, Group 3 and Group 4. Given the heterogeneity within and between medulloblastoma variants, surface marker profiles may be subtype-specific. Here, we employed a high throughput flow cytometry screen to identify differentially expressed cell surface markers in self-renewing vs. non-self-renewing SHH medulloblastoma cells. The top 25 markers were reduced to 4, CD271/p75NTR/NGFR, CD106/VCAM1, EGFR and CD171/NCAM-L1, by evaluating transcript levels in SHH tumors relative to samples representing the other variants. However, only CD271/p75NTR/NGFR and CD171/NCAM-L1 maintain differential expression between variants at the protein level. Functional characterization of CD271, a low affinity neurotrophin receptor, in cell lines and primary cultures suggested that CD271 selects for lower self-renewing progenitors or stem cells. Moreover, CD271 levels were negatively correlated with expression of SHH pathway genes. Our study reveals a novel role for CD271 in SHH medulloblastoma and suggests that targeting CD271 pathways could lead to the design of more selective therapies that lessen the broad impact of current treatments on developing nervous systems.

  3. 48 CFR 1352.271-88 - Guarantees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... when practicable, the Government shall afford the contractor an opportunity to effect such corrections... expiration of the time frame stated in this clause, it is not subject to the time limitations stated in this...

  4. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... concerned individuals or groups. This shall not include complaints alleging discrimination on the basis of..., Pennsylvania, Puerto Rico, Virginia, the Virgin Islands of the United States, and West Virginia: Mid-Atlantic...

  5. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals... rare earths to about 94-99 percent. The balance of tha alloy includes traces of other elements and one...

  6. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... this part are designed and promulgated to establish minimum standards which local zoning bylaws must... commercial and industrial use, other than existing commercial or industrial use not inconsistent with the...

  7. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... specific provisions of State law under which the applicant is organized; a copy of the applicant's articles... project. (i) Location and size of tract or tracts to be bought and/or developed. (ii) Number and size of... contribution to the project. (8) A map showing the location of and other supporting information on neighborhood...

  8. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... specific provisions of State law under which the applicant is organized; a copy of the applicant's articles... project. (i) Location and size of tract or tracts to be bought and/or developed. (ii) Number and size of... contribution to the project. (8) A map showing the location of and other supporting information on neighborhood...

  9. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... specific provisions of State law under which the applicant is organized; a copy of the applicant's articles... project. (i) Location and size of tract or tracts to be bought and/or developed. (ii) Number and size of... contribution to the project. (8) A map showing the location of and other supporting information on neighborhood...

  10. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... specific provisions of State law under which the applicant is organized; a copy of the applicant's articles... project. (i) Location and size of tract or tracts to be bought and/or developed. (ii) Number and size of... contribution to the project. (8) A map showing the location of and other supporting information on neighborhood...

  11. 12 CFR 271.6 - Processing requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ten working days of receipt of the request. In exceptional situations, the Secretary of the Committee... Committee, 20th and C Street, N.W., Washington, D.C. 20551; or sent by facsimile to the Secretary of the...

  12. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... one-line passengers; and (2) The traffic (including both local and beyond traffic) projected to flow..., Department estimates, and on traffic levels in the market at issue when such data are available. (b) The... proposed fare with the fare charged in other city-pair markets of similar distances and traffic densities...

  13. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... one-line passengers; and (2) The traffic (including both local and beyond traffic) projected to flow..., Department estimates, and on traffic levels in the market at issue when such data are available. (b) The... proposed fare with the fare charged in other city-pair markets of similar distances and traffic densities...

  14. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... one-line passengers; and (2) The traffic (including both local and beyond traffic) projected to flow..., Department estimates, and on traffic levels in the market at issue when such data are available. (b) The... proposed fare with the fare charged in other city-pair markets of similar distances and traffic densities...

  15. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... one-line passengers; and (2) The traffic (including both local and beyond traffic) projected to flow..., Department estimates, and on traffic levels in the market at issue when such data are available. (b) The... proposed fare with the fare charged in other city-pair markets of similar distances and traffic densities...

  16. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the top of an AOD vessel. Control device means the air pollution control equipment used to remove... vessel to the air pollution control device. Charge means the addition of iron and steel scrap or other... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  17. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the top of an AOD vessel. Control device means the air pollution control equipment used to remove... vessel to the air pollution control device. Charge means the addition of iron and steel scrap or other... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  18. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the top of an AOD vessel. Control device means the air pollution control equipment used to remove... vessel to the air pollution control device. Charge means the addition of iron and steel scrap or other... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  19. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the top of an AOD vessel. Control device means the air pollution control equipment used to remove... vessel to the air pollution control device. Charge means the addition of iron and steel scrap or other... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  20. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the top of an AOD vessel. Control device means the air pollution control equipment used to remove... vessel to the air pollution control device. Charge means the addition of iron and steel scrap or other... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  1. 40 CFR 408.271 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Steamed and Canned Oyster... this chapter shall apply to this subpart. (b) The term product shall mean the weight of the oyster meat...

  2. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... (i) A foam- or liquid-filled seal mounted in contact with the liquid (liquid-mounted seal). A liquid-mounted seal means a foam- or liquid-filled seal mounted in contact with the liquid between the wall of... are not in use. Rim space vents are to be set to open only when the internal floating roof is not...

  3. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... (i) A foam- or liquid-filled seal mounted in contact with the liquid (liquid-mounted seal). A liquid-mounted seal means a foam- or liquid-filled seal mounted in contact with the liquid between the wall of... are not in use. Rim space vents are to be set to open only when the internal floating roof is not...

  4. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... (i) A foam- or liquid-filled seal mounted in contact with the liquid (liquid-mounted seal). A liquid-mounted seal means a foam- or liquid-filled seal mounted in contact with the liquid between the wall of... are not in use. Rim space vents are to be set to open only when the internal floating roof is not...

  5. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... provide a projection below the liquid surface. Except for automatic bleeder vents, rim space vents, roof... floating roof means a cover that rests on the liquid surface (but not necessarily in complete contact with... floating on the liquid surface at all times, except during initial fill and during those intervals when the...

  6. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... provide a projection below the liquid surface. Except for automatic bleeder vents, rim space vents, roof... floating roof means a cover that rests on the liquid surface (but not necessarily in complete contact with... floating on the liquid surface at all times, except during initial fill and during those intervals when the...

  7. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture of...

  8. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture of...

  9. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture of...

  10. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture of...

  11. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... generated. Adequate power capacity must be provided for the operation of test and working equipment of the... restoration of power, the batteries must be restored to full charge within 24 hours. When primary power is... battery must permit continuation of normal operation for at least two hours under the normal operating...

  12. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... have a reliable source of suitable primary power, either from a power distribution system or locally... communications or reliable communications (at least a landline telephone) from the airport to the nearest Federal... reliable communications (at least a landline telephone) from the airport to the nearest Federal Aviation...

  13. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... specific provisions of State law under which the applicant is organized; a copy of the applicant's articles... services such as doctors, dentists, and hospitals. (9) If facilities such as water and sewage systems... or recommendation. County committees will not be used to review RHS loan applications. (e) Assembly...

  14. Functional characteristics of a novel SMAD4 mutation from thoracic aortic aneurysms (TAA).

    PubMed

    Wu, Lifei

    2017-09-10

    SMAD4 is as an essential mediator of the transforming growth factor β (TGF-β) signaling pathway, and dysregulated TGF-β signaling is linked with thoracic aortic aneurysms (TAAs). In this study, we functionally characterized the Smad4 S271N mutation (the mutation c. 812G>A in Smad4 results in the amino acid substitution Ser271Asn) that was isolated from TAA individuals. We first constructed wild-type human Smad4 and Smad4 S271N plasmids. These constructs were then transiently transfected into HEK293T cells, and subsequent real-time PCR and western blotting demonstrated that wild-type Smad4 and Smad4 S271N were successfully expressed in 293T cells. We found that HEK293T cells overexpressing Smad4 S271N showed a strong increase in both cytoplasmic and nuclear Smad4 protein levels in response to TGF-β1. Although TGF-β signaling was the same in wild-type Smad4- and Smad4 S271N-transfected cells following TGF-β1 exposure, interestingly, we observed that transient Smad4 S271N expression in HEK293T cells caused a significant basal activation of TGF-β signaling. These results indicated that Smad4 may not directly induce TAA; rather it may contribute to TAA in combination with other risk factors. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. 78 FR 59752 - Notice of a Record of Decision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ... ROD may be viewed online or during regular business hours at the following locations: 1. Online at www.... Grey may be contacted during business hours at (907) 271-5453 (telephone) and (907) 271-2851 (fax), or...

  16. 75 FR 38540 - Notice of Filing of Plats of Survey, Alaska

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ...: Stephen B. Hamrick, 907-271-5481, fax 907-271-4549, e-mail [email protected] . SUPPLEMENTARY INFORMATION.... Stephen B. Hamrick, Chief Cadastral Surveyor. [FR Doc. 2010-16220 Filed 7-1-10; 8:45 am] BILLING CODE 4310...

  17. Dose-Dependent Dual Role of PIT-1 (POU1F1) in Somatolactotroph Cell Proliferation and Apoptosis

    PubMed Central

    Jullien, Nicolas; Roche, Catherine; Brue, Thierry; Figarella-Branger, Dominique; Graillon, Thomas; Barlier, Anne; Herman, Jean-Paul

    2015-01-01

    To test the role of wtPIT-1 (PITWT) or PIT-1 (R271W) (PIT271) in somatolactotroph cells, we established, using inducible lentiviral vectors, sublines of GH4C1 somatotroph cells that allow the blockade of the expression of endogenous PIT-1 and/or the expression of PITWT or PIT271, a dominant negative mutant of PIT-1 responsible for Combined Pituitary Hormone Deficiency in patients. Blocking expression of endogenous PIT-1 induced a marked decrease of cell proliferation. Overexpressing PITWT twofold led also to a dose-dependent decrease of cell proliferation that was accompanied by cell death. Expression of PIT271 induced a strong dose-dependent decrease of cell proliferation accompanied by a very pronounced cell death. These actions of PIT271 are independent of its interaction/competition with endogenous PIT-1, as they were unchanged when expression of endogenous PIT-1 was blocked. All these actions are specific for somatolactotroph cells, and could not be observed in heterologous cells. Cell death induced by PITWT or by PIT271 was accompanied by DNA fragmentation, but was not inhibited by inhibitors of caspases, autophagy or necrosis, suggesting that this cell death is a caspase-independent apoptosis. Altogether, our results indicate that under normal conditions PIT-1 is important for the maintenance of cell proliferation, while when expressed at supra-normal levels it induces cell death. Through this dual action, PIT-1 may play a role in the expansion/regression cycles of pituitary lactotroph population during and after lactation. Our results also demonstrate that the so-called “dominant-negative” action of PIT271 is independent of its competition with PIT-1 or a blockade of the actions of the latter, and are actions specific to this mutant variant of PIT-1. PMID:25822178

  18. 75 FR 53333 - Notice of Filing of Plats of Survey, Alaska

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... CONTACT: Stephen B. Hamrick, 907-271-5481, fax 907-271-4549, e-mail [email protected] . SUPPLEMENTARY... guarantee that we will be able to do so. Authority: 43 U.S.C. 3; 53. Stephen B. Hamrick, Chief Cadastral...

  19. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  20. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  1. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  2. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  3. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  4. 38 CFR 3.271 - Computation of income.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... income. Recurring income means income which is received or anticipated in equal amounts and at regular... computation purposes. (2) Irregular income. Irregular income means income which is received or anticipated during a 12-month annualization period, but which is received in unequal amounts or at irregular...

  5. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Methyl Bromide 54 FR 41402-408 Apr. 6, 1990. Dec. 11, 1989 Listing Certain Hydrocarbons Produced by Free Radical Catalyzed Processes 54 FR 50968-978 June 11, 1990. Mar. 29, 1990 Toxicity characteristic 55 FR...

  6. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Methyl Bromide 54 FR 41402-408 Apr. 6, 1990. Dec. 11, 1989 Listing Certain Hydrocarbons Produced by Free Radical Catalyzed Processes 54 FR 50968-978 June 11, 1990. Mar. 29, 1990 Toxicity characteristic 55 FR...

  7. 38 CFR 3.271 - Computation of income.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... period (e.g., an inheritance). Pension computations of income will include nonrecurring income for a full... amount of a person's earnings or wages before any deductions are made for such things as taxes, insurance... goods sold, or expenditures for rent, taxes, and upkeep, or costs of repairs or replacements. The value...

  8. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under the... states the conditions under which spectrum is made available and licensed for the provision of wireless...

  9. 12 CFR 271.7 - Exemptions from disclosure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... its statutory functions; (2) Interfere with the orderly conduct of the foreign affairs of the United States; (3) Permit speculators or others to gain unfair profits or other unfair advantages by speculative...

  10. 8 CFR 271.1 - Procedures for inspections.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REASONABLE EFFORTS TO PREVENT THE UNAUTHORIZED ENTRY OF ALIENS BY THE OWNERS OF RAILROAD LINES, INTERNATIONAL... director determines that preventive measures are inadequate, he or she shall advise the owners or operators... results of his or her review. (c) Preventive measures and certification. Upon a determination by the...

  11. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... facilities to be owned by a Federal department or agency. You may use a PLA in leasehold arrangement... than $5 million. Project Labor Agreement (PLA) means an agreement between the contractor, subcontractors, and the union(s) representing workers. Under a PLA, the contractor and subcontractors on a...

  12. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... significant project means a Federal construction project with a total cost to the Federal Government of more... framework for labor-management cooperation to advance the Government's procurement interest in cost, efficiency, and quality. (d) Policy. (1) You may, on a project-by-project basis, use a PLA on a large and...

  13. 14 CFR 21.271 - Airworthiness approval tags.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... may issue an airworthiness approval tag (FAA Form 8130-3) for each engine and propeller covered by... shall be stamped on the engine or propeller identification data place instead of issuing an...

  14. 14 CFR 21.271 - Airworthiness approval tags.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... (FAA Form 8130-3) for each engine and propeller covered by § 21.251(b)(4), and may issue an... Limitation Record, the production certification number shall be stamped on the engine or propeller...

  15. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  16. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  17. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  18. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  19. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  20. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... available data pertaining to the number of people participating in the program and the amounts of benefits... for completing the processing of such cases shall be five calendar days or the end of the month of...

  1. 40 CFR 271.14 - Requirements for permitting.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... requirements: (a) Section 270.1(c)(1)—(Specific inclusions); (b) Section 270.4—(Effect of permit); (c) Section...—(Signatories); (f) Section 270.12—(Confidential information); (g) Section 270.13—(Contents of part A); (h... modification); (o) Section 270.43—(Permit termination); (p) Section 270.50—(Duration); (q) Section 270.60...

  2. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., and 805-806 MHz. (3) 698-746 MHz. (4) 1390-1392 MHz. (5) 1392-1395 MHz and 1432-1435 MHz. (6) 1670-1675 MHz. (7) [Reserved] (8) 1710-1755 MHz and 2110-2155 MHz. (9) 2495-2690 MHz. (c) Scope. The rules...

  3. Photocopy of drawing located at National Archives, San Bruno, California ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of drawing located at National Archives, San Bruno, California (Navy 271-A-17). Minsy building 271 record 1st floor plan; N.D. - Mare Island Naval Shipyard, Mechanics Shop, Waterfront Avenue, west side between A Street & Third Street, Vallejo, Solano County, CA

  4. Mapping selected general literature of international nursing.

    PubMed

    Shams, Marie-Lise Antoun; Dixon, Lana S

    2007-01-01

    This study, part of a wider project to map the literature of nursing, identifies core journals cited in non-US nursing journals and determines the extent of their coverage by indexing services. Four general English-language journals were analyzed for format types and publication dates. Core titles were identified and nine bibliographic databases were scanned for indexing coverage. Findings show that 57.5% (13,391/23,271) of the cited references from the 4 core journals were to journal articles, 27.8% (6,471/23,271) to books, 9.5% (2,208/23,271) to government documents, 4.9% (1,131/23,271) to miscellaneous sources, and less than 1% (70/23,271) to Internet resources. Eleven journals produced one-third of the citations; the next third included 146 journals, followed by a dispersion of 1,622 titles. PubMed received the best database coverage scores, followed by CINAHL and Science Citation Index. None of the databases provided complete coverage of all 11 core titles. The four source journals contain a diverse group of cited references. The currency of citations to government documents makes these journals a good source for regulatory and legislative awareness. Nurses consult nursing and biomedical journals and must search both nursing and biomedical databases to cover the literature.

  5. 76 FR 8626 - Revision of Class E Airspace; Shungnak, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ...-1104; Airspace Docket No. 10-AAL-19] Revision of Class E Airspace; Shungnak, AK AGENCY: Federal... Shungnak, AK, to accommodate amended Standard Instrument Approach Procedures (SIAPs) at the Shungnak..., Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850; e- mail...

  6. Eielson AFB, Alaska. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    DTIC Science & Technology

    1987-12-01

    26/ 609 111 301 13/ 369 13O 271 1 301 281 60 j I :7T 239 259 9/ 309 1lt 271 41 299 29 309 26/ 231 26/ 311 26/ 229 261 281 29O 629 271 61 271 6 ?I I ISO ... 27001 90.9 93. 9 98.8 95.2 96.8 96.9 96.1 96.3 96.6 96.6 96.6 96.6 96.6 96.6 96.6 96.6 b E 1803o 91.1 94.2 95.1 95.8 96.6 96.6 96.8 97.0 97.2 97.2...91.9 91.6 91.6 91.6 91.9 91.9 91.9 92.0 92.0 92.0 92.0 2.0 92.0 92.0 6E 27001 67.0 92.0 92.6 92.9 93.3 93.3 93.3 93.3 93.3 93.0 93.9 91.4 93.0 93.4

  7. Alzheimer’s Disease in Social Media: Content Analysis of YouTube Videos

    PubMed Central

    Tang, Weizhou; Olscamp, Kate; Friedman, Daniela B

    2017-01-01

    Background Approximately 5.5 million Americans are living with Alzheimer’s disease (AD) in 2017. YouTube is a popular platform for disseminating health information; however, little is known about messages specifically regarding AD that are being communicated through YouTube. Objective This study aims to examine video characteristics, content, speaker characteristics, and mobilizing information (cues to action) of YouTube videos focused on AD. Methods Videos uploaded to YouTube from 2013 to 2015 were searched with the term “Alzheimer’s disease” on April 30th, 2016. Two coders viewed the videos and coded video characteristics (the date when a video was posted, Uniform Resource Locator, video length, audience engagement, format, author), content, speaker characteristics (sex, race, age), and mobilizing information. Descriptive statistics were used to examine video characteristics, content, audience engagement (number of views), speaker appearances in the video, and mobilizing information. Associations between variables were examined using Chi-square and Fisher’s exact tests. Results Among the 271 videos retrieved, 25.5% (69/271) were posted by nonprofit organizations or universities. Informal presentations comprised 25.8% (70/271) of all videos. Although AD symptoms (83/271, 30.6%), causes of AD (80/271, 29.5%), and treatment (76/271, 28.0%) were commonly addressed, quality of life of people with AD (34/271, 12.5%) had more views than those more commonly-covered content areas. Most videos featured white speakers (168/187, 89.8%) who were adults aged 20 years to their early 60s (164/187, 87.7%). Only 36.9% (100/271) of videos included mobilizing information. Videos about AD symptoms were significantly less likely to include mobilizing information compared to videos without AD symptoms (23/83, 27.7% vs 77/188, 41.0% respectively; P=.03). Conclusions This study contributes new knowledge regarding AD messages delivered through YouTube. Findings of the current

  8. Alzheimer's Disease in Social Media: Content Analysis of YouTube Videos.

    PubMed

    Tang, Weizhou; Olscamp, Kate; Choi, Seul Ki; Friedman, Daniela B

    2017-10-19

    Approximately 5.5 million Americans are living with Alzheimer's disease (AD) in 2017. YouTube is a popular platform for disseminating health information; however, little is known about messages specifically regarding AD that are being communicated through YouTube. This study aims to examine video characteristics, content, speaker characteristics, and mobilizing information (cues to action) of YouTube videos focused on AD. Videos uploaded to YouTube from 2013 to 2015 were searched with the term "Alzheimer's disease" on April 30th, 2016. Two coders viewed the videos and coded video characteristics (the date when a video was posted, Uniform Resource Locator, video length, audience engagement, format, author), content, speaker characteristics (sex, race, age), and mobilizing information. Descriptive statistics were used to examine video characteristics, content, audience engagement (number of views), speaker appearances in the video, and mobilizing information. Associations between variables were examined using Chi-square and Fisher's exact tests. Among the 271 videos retrieved, 25.5% (69/271) were posted by nonprofit organizations or universities. Informal presentations comprised 25.8% (70/271) of all videos. Although AD symptoms (83/271, 30.6%), causes of AD (80/271, 29.5%), and treatment (76/271, 28.0%) were commonly addressed, quality of life of people with AD (34/271, 12.5%) had more views than those more commonly-covered content areas. Most videos featured white speakers (168/187, 89.8%) who were adults aged 20 years to their early 60s (164/187, 87.7%). Only 36.9% (100/271) of videos included mobilizing information. Videos about AD symptoms were significantly less likely to include mobilizing information compared to videos without AD symptoms (23/83, 27.7% vs 77/188, 41.0% respectively; P=.03). This study contributes new knowledge regarding AD messages delivered through YouTube. Findings of the current study highlight a potential gap between available information

  9. 76 FR 8627 - Revision of Class E Airspace; Platinum, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ...-1105; Airspace Docket No. 10-AAL-20] Revision of Class E Airspace; Platinum, AK AGENCY: Federal... Platinum, AK, to accommodate the addition of a Standard Instrument Approach Procedure (SIAP), at the... 7th Avenue, Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850; e...

  10. 75 FR 12680 - Revision of Class E Airspace; Scammon Bay, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-17

    ...-1038; Airspace Docket No. 09-AAL-19] Revision of Class E Airspace; Scammon Bay, AK AGENCY: Federal... Scammon Bay, AK, to accommodate new Area Navigation (RNAV) Standard Instrument Approach Procedures (SIAPs..., 222 West 7th Avenue, Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271...

  11. Prothrombin activation on the activated platelet surface optimizes expression of procoagulant activity

    PubMed Central

    Wood, Jeremy P.; Silveira, Jay R.; Maille, Nicole M.; Haynes, Laura M.

    2011-01-01

    Effective hemostasis relies on the timely formation of α-thrombin via prothrombinase, a Ca2+-dependent complex of factors Va and Xa assembled on the activated platelet surface, which cleaves prothrombin at Arg271 and Arg320. Whereas initial cleavage at Arg271 generates the inactive intermediate prethrombin-2, initial cleavage at Arg320 generates the enzymatically active intermediate meizothrombin. To determine which of these intermediates is formed when prothrombin is processed on the activated platelet surface, the cleavage of prothrombin, and prothrombin mutants lacking either one of the cleavage sites, was monitored on the surface of either thrombin- or collagen-activated platelets. Regardless of the agonist used, prothrombin was initially cleaved at Arg271 generating prethrombin-2, with α-thrombin formation quickly after via cleavage at Arg320. The pathway used was independent of the source of factor Va (plasma- or platelet-derived) and was unaffected by soluble components of the platelet releasate. When both cleavage sites are presented within the same substrate molecule, Arg271 effectively competes against Arg320 (with an apparent IC50 = 0.3μM), such that more than 90% to 95% of the initial cleavage occurs at Arg271. We hypothesize that use of the prethrombin-2 pathway serves to optimize the procoagulant activity expressed by activated platelets, by limiting the anticoagulant functions of the alternate intermediate, meizothrombin. PMID:21131592

  12. Prothrombin activation on the activated platelet surface optimizes expression of procoagulant activity.

    PubMed

    Wood, Jeremy P; Silveira, Jay R; Maille, Nicole M; Haynes, Laura M; Tracy, Paula B

    2011-02-03

    Effective hemostasis relies on the timely formation of α-thrombin via prothrombinase, a Ca(2+)-dependent complex of factors Va and Xa assembled on the activated platelet surface, which cleaves prothrombin at Arg271 and Arg320. Whereas initial cleavage at Arg271 generates the inactive intermediate prethrombin-2, initial cleavage at Arg320 generates the enzymatically active intermediate meizothrombin. To determine which of these intermediates is formed when prothrombin is processed on the activated platelet surface, the cleavage of prothrombin, and prothrombin mutants lacking either one of the cleavage sites, was monitored on the surface of either thrombin- or collagen-activated platelets. Regardless of the agonist used, prothrombin was initially cleaved at Arg271 generating prethrombin-2, with α-thrombin formation quickly after via cleavage at Arg320. The pathway used was independent of the source of factor Va (plasma- or platelet-derived) and was unaffected by soluble components of the platelet releasate. When both cleavage sites are presented within the same substrate molecule, Arg271 effectively competes against Arg320 (with an apparent IC(50) = 0.3μM), such that more than 90% to 95% of the initial cleavage occurs at Arg271. We hypothesize that use of the prethrombin-2 pathway serves to optimize the procoagulant activity expressed by activated platelets, by limiting the anticoagulant functions of the alternate intermediate, meizothrombin.

  13. 75 FR 62458 - Revision of Class E Airspace; Tanana, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-12

    ...-0588 Airspace Docket No. 10-AAL-16] Revision of Class E Airspace; Tanana, AK AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action revises Class E airspace at Tanana, AK. The... West 7th Avenue, Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850...

  14. 75 FR 32272 - Revision of Class E Airspace; Kaltag, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ...-0082; Airspace Docket No. 10-AAL-4] Revision of Class E Airspace; Kaltag, AK AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action revises Class E airspace at Kaltag, AK, to..., Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850; e-mail: [email protected

  15. Isolation and characterisation of mesenchymal stem/stromal cells in the ovine endometrium.

    PubMed

    Letouzey, Vincent; Tan, Ker Sin; Deane, James A; Ulrich, Daniela; Gurung, Shanti; Ong, Y Rue; Gargett, Caroline E

    2015-01-01

    Mesenchymal stem/stromal cells (MSC) were recently discovered in the human endometrium. These cells possess key stem cell properties and show promising results in small animal models when used for preclinical tissue engineering studies. A small number of surface markers have been identified that enrich for MSC from bone marrow and human endometrium, including the Sushi Domain-containing 2 (SUSD2; W5C5) and CD271 markers. In preparation for developing a large animal preclinical model for urological and gynecological tissue engineering applications we aimed to identify and characterise MSC in ovine endometrium and determine surface markers to enable their prospective isolation. Ovine endometrium was obtained from hysterectomised ewes following progesterone synchronisation, dissociated into single cell suspensions and tested for MSC surface markers and key stem cell properties. Purified stromal cells were obtained by flow cytometry sorting with CD49f and CD45 to remove epithelial cells and leukocytes respectively, and MSC properties investigated. There was a small population CD271+ stromal cells (4.5 ± 2.3%) in the ovine endometrium. Double labelling with CD271 and CD49f showed that the sorted CD271+CD49f- stromal cell population possessed significantly higher cloning efficiency, serial cloning capacity and a qualitative increased ability to differentiate into 4 mesodermal lineages (adipocytic, smooth muscle, chondrocytic and osteoblastic) than CD271-CD49f- cells. Immunolabelling studies identified an adventitial perivascular location for ovine endometrial CD271+ cells. This is the first study to characterise MSC in the ovine endometrium and identify a surface marker profile identifying their location and enabling their prospective isolation. This knowledge will allow future preclinical studies with a large animal model that is well established for pelvic organ prolapse research.

  16. Evaluation of acetone vapors toxicity on Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae) eggs.

    PubMed

    Pourmirza, Ali Asghr; Nasab, Fershteh Sadeghi; Zadeh, Abas Hossein

    2007-08-01

    The efficacy of acetone vapors against carefully aged eggs of Plodia interpunctella (Hubner) at 17+/-1 and 27+/-1 degrees C at different dosage levels of acetone over various exposure times was determined. Acetone was found to be toxic to Indian meal moth eggs. Considerable variation in the susceptibility of different age groups of eggs was apparent in the fiducial limits of the LD50 values. An inverse relationship between LD50 values and exposure times was observed in age groups of tested eggs. At 27+/-1 degrees C and 24 h exposure period, eggs aged 1-2 day-old were more tolerant to acetone than other age groups, followed by 0-1 day-old, 2-3 day-old and 3-4 day-old eggs. A similar pattern of susceptibility of eggs was observed at 72 h exposure. In all bioassays, eggs exposed to higher dosages of acetone developed at smaller rate. This was significant for the eggs, which were exposed to the highest dosage for 24 h. Increasing the temperature from 17+/-1 to 27+/-1 degrees C greatly increased the efficacy of acetone. At 27+/-1 degrees C eggs of P. interpunctella were killed by less than one-third of the dosage required for control at 17+/-1 degrees C. Acetone achieved 50% mortality with a dosage of 82.76 mg L(-1) in 1-2 day-old eggs at 27+/-1 degrees C. At this temperature hatching was retarded and greatly diminished when eggs aged 1-2 day-old were exposed to 80 mg L(-1) of acetone for the 24 h exposure period. There was no evidence of a hatch delay longer than the time spent under vapors for eggs exposed at 17+/-1 or 27+/-1 degrees C, indicating that some development must have occurred under fumigation.

  17. Decay properties of 256-339Ds superheavy nuclei

    NASA Astrophysics Data System (ADS)

    Santhosh, K. P.; Nithya, C.

    2017-09-01

    The decay properties of 84 isotopes of darmstadtium superheavy nuclei ( Z = 110) have been studied using various theoretical models. The proton emission half-lives, the alpha decay half-lives, the spontaneous fission half-lives and the cluster decay half-lives of all the isotopes are evaluated. The one-proton emission half-lives and the alpha decay half-lives are predicted using the Coulomb and proximity potential model for deformed nuclei (CPPMDN). The calculated alpha half-lives are compared with the available experimental results as well as with the predictions of other theoretical models. The predicted half-lives matches well with the experimental results. The one-proton half-lives are also compared with the predictions using other formalisms. The shell-effect-dependent formula of Santhosh et al. has been employed for calculating the spontaneous fission half-lives. A theoretical comparison of spontaneous fission half-lives with four different formalisms is performed. By comparing the one-proton emission half-lives, the alpha decay half-lives and the spontaneous fission half-lives decay modes are predicted for all the isotopes of Ds. It is seen that the isotopes within the range 256 ≤ A ≤ 263 and 279 ≤ A ≤ 339 decay through spontaneous fission and the isotopes 264 ≤ A ≤ 278 exhibit alpha decay. Cluster decay half-lives are calculated using different models including the Coulomb and proximity potential (CPPM), for determining the magicities in the superheavy region. The effect of magicity at N = 184 and N = 202 were confirmed from the plot of log_{10}T_{1/2} versus neutron number of the daughter nuclei for the emission of different clusters. We hope that the systematic and detailed study of all the possible decay modes of 256-339Ds using various theoretical models will be helpful in the experimental identification of the isotopes of the element in the future.

  18. Protein Engineering and Homologous Expression of Serratia marcescens Lipase for Efficient Synthesis of a Pharmaceutically Relevant Chiral Epoxyester.

    PubMed

    Chen, Ke-Cai; Zheng, Ming-Min; Pan, Jiang; Li, Chun-Xiu; Xu, Jian-He

    2017-10-01

    The lipase isolated from Serratia marcescens (LipA) is a useful biocatalyst for kinetic resolution of a pharmaceutically relevant epoxyester, (±)-3-(4'-methoxyphenyl) glycidic acid methyl ester [(±)-MPGM], to afford optically pure (-)-MPGM, a key intermediate for the synthesis of diltiazem hydrochloride. Two mutants, LipA L315S and LipA S271F , were identified from the combinatorial saturation mutation library of 14 amino acid residues lining the substrate-binding pocket. LipA L315S , LipA S271F , and their combination LipA L315S/S271F showed 2.6-, 2.2-, and 4.6-fold improvements in their specific activities towards para-nitrophenyl butyrate (pNPB), respectively. Among these positive mutants, LipA S271F displayed a 3.5-fold higher specific activity towards the pharmaco substrate (±)-MPGM. Kinetic study showed that the improvement in catalytic efficiency of LipA S271F against (±)-MPGM was mainly resulted from the enhanced affinity between substrate and enzyme, as indicated by the decrease of K m . Furthermore, to address the insoluble expression issue in Escherichia coli, the homologous expression of LipA gene in S. marcescens was achieved by introducing it into an expression vector pUC18, resulting in ca. 20-fold higher lipase production. The significantly improved volumeric production and specific activity of S. marcescens lipase make it very attractive as a new-generation biocatalyst for more efficient and economical manufacturing of (-)-MPGM.

  19. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF... the levels of nutrition among low-income households. Section 2 of the Food Stamp Act of 1977 states... utilization of food in establishing and maintaining adequate national levels of nutrition will promote the...

  20. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF... the levels of nutrition among low-income households. Section 2 of the Food Stamp Act of 1977 states... utilization of food in establishing and maintaining adequate national levels of nutrition will promote the...

  1. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF... the levels of nutrition among low-income households. Section 2 of the Food Stamp Act of 1977 states... utilization of food in establishing and maintaining adequate national levels of nutrition will promote the...

  2. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  3. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  4. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  5. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  6. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  7. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the health and well being of the Nation's population by raising the levels of nutrition among low-income households. Section 2 of the Food and Nutrition Act of 2008 states, in part: Congress hereby finds... Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF...

  8. Translations on Narcotics and Dangerous Drugs No. 271

    DTIC Science & Technology

    1976-11-11

    Worth 25,000 Kyats Seized in Rangoon (MYANMA ALIN, 18 Oct 76) 10 Marihuana Burned in Okto (MYANMA ALIN, 2 Oct 76) 11 MALAYSIA American... Marihuana , Cocaine Pushers, Addicts Arrested (EL TIEMPO, 23 Sep 76) 29 Briefs Drug Ring Smashed 31 Drug Hauls and Arrests 31 Drug Traffickers Arrested...lead of the Labor Governments in New South Wales and South Australia in moves to legalise marihuana ," he said. A submission by the Australian

  9. 48 CFR 1352.271-81 - Discharge of liens.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) The contractor shall immediately discharge or cause to be discharged any lien or right in rem of any... done or materials furnished under the contract. If any such lien or right in rem is not immediately discharged, the Government may discharge or cause to be discharged such lien or right at the expense of the...

  10. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI employee... Responsibility (OPR), the Department's Office of Inspector General (OIG), the FBI Office of Professional Responsibility (FBI OPR), the FBI Inspection Division (FBI-INSD) Internal Investigations Section (collectively...

  11. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI employee... Responsibility (OPR), the Department's Office of Inspector General (OIG), the FBI Office of Professional Responsibility (FBI OPR), the FBI Inspection Division (FBI-INSD) Internal Investigations Section (collectively...

  12. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI employee... Responsibility (OPR), the Department's Office of Inspector General (OIG), the FBI Office of Professional Responsibility (FBI OPR), the FBI Inspection Division (FBI-INSD) Internal Investigations Section (collectively...

  13. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI employee... Responsibility (OPR), the Department's Office of Inspector General (OIG), the FBI Office of Professional Responsibility (FBI OPR), the FBI Inspection Division (FBI-INSD) Internal Investigations Section (collectively...

  14. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI employee... Responsibility (OPR), the Department's Office of Inspector General (OIG), the FBI Office of Professional Responsibility (FBI OPR), the FBI Inspection Division (FBI-INSD) Internal Investigations Section (collectively...

  15. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... individuals of any cetacean species (not including mother/calf pairs, unless of species of concern listed in... one another. (ii) A single individual or mother/calf pair of any of the following marine mammals of...

  16. Publications - GMC 271 | Alaska Division of Geological & Geophysical

    Science.gov Websites

    ') and of core (9,683-9,694') from the ARCO Alaska Inc. Tulaga #1 well Authors: Unknown Publication Date Reference Unknown, 1996, Vitrinite reflectance maceral data of cuttings (1,200-11,742') and of core (9,683

  17. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Manpower Loading Curve. (4) Trade Manning Curves. (5) Subcontracting List. (b) The Production Schedule... events, and activities and shall clearly identify the critical path. The Total Manpower Loading Curve... deviation in the Production Schedule which results in a delay in the completion of work on a vessel past the...

  18. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Manpower Loading Curve. (4) Trade Manning Curves. (5) Subcontracting List. (b) The Production Schedule... events, and activities and shall clearly identify the critical path. The Total Manpower Loading Curve... deviation in the Production Schedule which results in a delay in the completion of work on a vessel past the...

  19. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Manpower Loading Curve. (4) Trade Manning Curves. (5) Subcontracting List. (b) The Production Schedule... events, and activities and shall clearly identify the critical path. The Total Manpower Loading Curve... deviation in the Production Schedule which results in a delay in the completion of work on a vessel past the...

  20. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Manpower Loading Curve. (4) Trade Manning Curves. (5) Subcontracting List. (b) The Production Schedule... events, and activities and shall clearly identify the critical path. The Total Manpower Loading Curve... deviation in the Production Schedule which results in a delay in the completion of work on a vessel past the...

  1. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Manpower Loading Curve. (4) Trade Manning Curves. (5) Subcontracting List. (b) The Production Schedule... events, and activities and shall clearly identify the critical path. The Total Manpower Loading Curve... deviation in the Production Schedule which results in a delay in the completion of work on a vessel past the...

  2. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... agricultural economy, as well as result in more orderly marketing and distribution of foods. To alleviate such... households to obtain a more nutritious diet through normal channels of trade by increasing food purchasing...

  3. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is required...

  4. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is required...

  5. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is required...

  6. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is required...

  7. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is required...

  8. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... components of end items or services be purchased separately so small firms may compete; (f) Making a... final decision on a proposed non-competitive procurement action, and as part of his/her findings and... awareness by the technical staff of the availability of small business concerns; (l) Making available to SDB...

  9. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Insurance (APR 2010) (a) The contractor shall exercise reasonable care and use its best efforts to prevent accidents, injury or damage to all employees, persons and property, in and about the work, and to the vessel... at the plant or elsewhere, arising or growing out of the performance of the work, except where the...

  10. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2013-10-01 2013-10-01 false Status conference. 1.733 Section 1.733...

  11. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2010-10-01 2010-10-01 false Status conference. 1.733 Section 1.733...

  12. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2014-10-01 2014-10-01 false Status conference. 1.733 Section 1.733...

  13. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2011-10-01 2011-10-01 false Status conference. 1.733 Section 1.733...

  14. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2012-10-01 2012-10-01 false Status conference. 1.733 Section 1.733...

  15. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 9 2012-10-01 2012-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  16. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 9 2013-10-01 2013-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  17. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 9 2014-10-01 2014-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  18. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  19. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 9 2011-10-01 2011-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  20. Addendum to the Closure Report for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems Nevada Test Site, Nevada, Revision 0

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lynn Kidman

    This document constitutes an addendum to the August 2004, Closure Report for Corrective Action Unit 271, Areas 25, 26, and 27 Septic Systems as described in the document Recommendations and Justifications for Modifications for Use Restrictions Established under the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office Federal Facility Agreement and Consent Order (UR Modification document) dated February 2008. The UR Modification document was approved by NDEP on February 26, 2008. The approval of the UR Modification document constituted approval of each of the recommended UR modifications. In conformance with the UR Modification document, this addendum consistsmore » of: • This cover page that refers the reader to the UR Modification document for additional information • The cover and signature pages of the UR Modification document • The NDEP approval letter • The corresponding section of the UR Modification document This addendum provides the documentation justifying the modification of the UR for CAS 27-05-02, Leachfield. This UR was established as part of a Federal Facility Agreement and Consent Order (FFACO) corrective action and is based on the presence of contaminants at concentrations greater than the action levels established at the time of the initial investigation (FFACO, 1996; as amended August 2006). Since this UR was established, practices and procedures relating to the implementation of risk-based corrective actions (RBCA) have changed. Therefore, this UR was re-evaluated against the current RBCA criteria as defined in the Industrial Sites Project Establishment of Final Action Levels (NNSA/NSO, 2006c). This re-evaluation consisted of comparing the original data (used to define the need for the UR) to risk-based final action levels (FALs) developed using the current Industrial Sites RBCA process. The re-evaluation resulted in a recommendation to modify the UR to an administrative UR. Administrative URs differ

  1. Decision Aiding and Coordination in Decision-Making Organizations.

    DTIC Science & Technology

    1988-01-01

    30111 211100 30131 281 150 90 131 231 100 40 9 151 241 90 30151 241 90 30181 331 150 90 161 261 100 40 10 181 271 90 30 181 271 90 30231 381 150 90 181...check mark is present, the delayed execution will take place. I’, Hm ~One: function: Used to specify that the execution is implemented one firing at a

  2. An Overview of Production Systems

    DTIC Science & Technology

    1975-10-01

    DISTRIBUTED BY: Matonal Tochnica! Infonu srice U. S. DEPARTMENT OF COMMERCE 028143 Stanford Artificil Inteligence Laboratory October 1975 Memo AIM-271...ORGANIZATION NAMEL AND ADDRESS 18. PROGRAM ELEMENT. PROJECT. TASK Artificial Intelligence Laboratory AE OKUI UBR Stanford University ARPA Order 249...014-64011I j SEC-jRITY CLASSIFICATION OF THIS PAGE (When, Data bHISP011 A Stanford Artificial ktteligncs Laboratory October 1975 Memo AIM-271 Computer

  3. Electronic State Decomposition of Energetic Materials and Model Systems

    DTIC Science & Technology

    2010-11-17

    Nitromethane at 226 nm and 271 nm at both Nanosecond and Femtosecond Temporal Scales," J. Phys. Chem. A 113, 85 (2009). Y. Q. Guo, A. Bhattacharya and E...less "energetic". 8. Photodissociation Dynamics of Nitromethane at 226 and 271 nm at Both Nanosecond and Femtosecond Time Scales Photodissociation...of nitromethane has been investigated for decades both theoretically and experimentally; however, as a whole picture, the dissociation dynamics for

  4. Identification of essential histidine residues in the active site of Escherichia coli xylose (glucose) isomerase.

    PubMed Central

    Batt, C A; Jamieson, A C; Vandeyar, M A

    1990-01-01

    Two conserved histidine residues (His-101 and His-271) appear to be essential components in the active site of the enzyme xylose (glucose) isomerase (EC 5.3.1.5). These amino acid residues were targeted for mutagenesis on the basis of sequence homology among xylose isomerases isolated from Escherichia coli, Bacillus subtilis, Ampullariella sp. strain 3876, and Streptomyces violaceus-niger. Each residue was selectively replaced by site-directed mutagenesis and shown to be essential for activity. No measurable activity was observed for any mutations replacing either His-101 or His-271. Circular dichroism measurements revealed no significant change in the overall conformation of the mutant enzymes, and all formed dimers similar to the wild-type enzyme. Mutations at His-271 could be distinguished from those at His-101, since the former resulted in a thermolabile protein whereas no significant change in heat stability was observed for the latter. Based upon these results and structural data recently reported, we speculate that His-101 is the catalytic base mediating the reaction. Replacement of His-271 may render the enzyme thermolabile, since this residue appears to be a ligand for one of the metal ions in the active site of the enzyme. Images PMID:2405386

  5. Development of a Procurement Task Classification Scheme.

    DTIC Science & Technology

    1987-12-01

    Office of Sci- entific Research, Arlington, Virginia, January 1970. Tornow , Walter W . and Pinto, Patrick R. "The Development of a Man- agerial Job...classification. [Ref. 4:271 -. 20 6 %° w Numerical taxonomy proponents hold [Ref. 4:271, ... that the relationships of contiguity and similarity should be...solving. 22 W i * These primitive categories are based on a sorting of learning pro- cesses into classes that have obvious differences at the

  6. Carboxylesterase-mediated insecticide resistance: Quantitative increase induces broader metabolic resistance than qualitative change.

    PubMed

    Cui, Feng; Li, Mei-Xia; Chang, Hai-Jing; Mao, Yun; Zhang, Han-Ying; Lu, Li-Xia; Yan, Shuai-Guo; Lang, Ming-Lin; Liu, Li; Qiao, Chuan-Ling

    2015-06-01

    Carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (OP) insecticides. Carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (G/A151D) or from tryptophan to leucine at the 271 site (W271L), following the numbering of Drosophila melanogaster AChE. Qualitative mechanism has been observed in few species. However, whether this carboxylesterase mutation mechanism is prevalent in insects remains unclear. In this study, wild-type, G/A151D and W271L mutant carboxylesterases from Culex pipiens and Aphis gossypii were subjected to germline transformation and then transferred to D. melanogaster. These germlines were ubiquitously expressed as induced by tub-Gal4. In carboxylesterase activity assay, the introduced mutant carboxylesterase did not enhance the overall carboxylesterase activity of flies. This result indicated that G/A151D or W271L mutation disrupted the original activities of the enzyme. Less than 1.5-fold OP resistance was only observed in flies expressing A. gossypii mutant carboxylesterases compared with those expressing A. gossypii wild-type carboxylesterase. However, transgenic flies universally showed low resistance to OP insecticides compared with non-transgenic flies. The flies expressing A. gossypii W271L mutant esterase exhibited 1.5-fold resistance to deltamethrin, a pyrethroid insecticide compared with non-transgenic flies. The present transgenic Drosophila system potentially showed that a quantitative increase in carboxylesterases induced broader resistance of insects to insecticides than a qualitative change. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... surveillance procedures to determine, independent of information supplied by regulated persons, compliance or... reporting forms and other forms supplying monitoring data; and (iii) Verify the adequacy of sampling... shall be taken and other information shall be gathered in a manner (e.g., using proper “chain of custody...

  8. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... surveillance procedures to determine, independent of information supplied by regulated persons, compliance or... reporting forms and other forms supplying monitoring data; and (iii) Verify the adequacy of sampling... shall be taken and other information shall be gathered in a manner (e.g., using proper “chain of custody...

  9. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... surveillance procedures to determine, independent of information supplied by regulated persons, compliance or... reporting forms and other forms supplying monitoring data; and (iii) Verify the adequacy of sampling... shall be taken and other information shall be gathered in a manner (e.g., using proper “chain of custody...

  10. 12 CFR 271.9 - Fee schedules; waiver of fees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Secretary receives the required payment. (3) Late charges. The Secretary may assess interest charges when fee payment is not made within 30 days of the date on which the billing was sent. Interest is at the rate prescribed in 31 U.S.C. 3717 and accrues from the date of the billing. (c) Categories of uses. The...

  11. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... acquisition threshold shall be screened by SDB, with the exception of: (1) Class set-asides and those... the original and 2 copies to SDB within one working day of receipt by the contracting activity of a... appropriate) prior to submittal to SDB. (c) Screening of Form USAID 1410-14 by SDB. (1) SDB will screen the...

  12. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... acquisition threshold shall be screened by SDB, with the exception of: (1) Class set-asides and those... the original and 2 copies to SDB within one working day of receipt by the contracting activity of a... appropriate) prior to submittal to SDB. (c) Screening of Form USAID 1410-14 by SDB. (1) SDB will screen the...

  13. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... acquisition threshold shall be screened by SDB, with the exception of: (1) Class set-asides and those... the original and 2 copies to SDB within one working day of receipt by the contracting activity of a... appropriate) prior to submittal to SDB. (c) Screening of Form USAID 1410-14 by SDB. (1) SDB will screen the...

  14. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... acquisition threshold shall be screened by SDB, with the exception of: (1) Class set-asides and those... the original and 2 copies to SDB within one working day of receipt by the contracting activity of a... appropriate) prior to submittal to SDB. (c) Screening of Form USAID 1410-14 by SDB. (1) SDB will screen the...

  15. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... organically produced products or ingredients with the substance used. (e) The handler of an organic handling... MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic Production and Handling...

  16. Aeronautical engineering: A continuing bibliography with indexes (supplement 271)

    NASA Technical Reports Server (NTRS)

    1991-01-01

    This bibliography lists 666 reports, articles, and other documents introduced into the NASA scientific and technical information system in October, 1991. Subject coverage includes design, construction and testing of aircraft and aircraft engines; aircraft components, equipment and systems; ground support systems; and theoretical and applied aspects of aerodynamics and general fluid dynamics.

  17. Worldwide Report, Telecommunications Policy, Research and Development, No. 271

    DTIC Science & Technology

    1983-05-17

    and not as educational or cultural instru- ments is the fact that the director of Radio Educacion is now appointed by the Govern- ment Secretariat...Journalists, Froyland Rascon of Radio Educacion , Jose Alvarez Icaza of Cencos [National Mass Media Center]; Leopoldo de Gyves, the mayor of Juchitan...board, especially for the new television station. Programmes of a political nature would also take up air-time but if South Africa objected to

  18. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... marketers which are equivalent to those under subpart H of part 279 of this chapter; and (g) Standards for...) and (b) Sec. 279.66(b) Sec. 279.72(b) 1 Contains additional new definitions that were not included in...

  19. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... surveillance procedures to determine, independent of information supplied by regulated persons, compliance or... reporting forms and other forms supplying monitoring data; and (iii) Verify the adequacy of sampling... shall be taken and other information shall be gathered in a manner (e.g., using proper “chain of custody...

  20. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... surveillance procedures to determine, independent of information supplied by regulated persons, compliance or... reporting forms and other forms supplying monitoring data; and (iii) Verify the adequacy of sampling... shall be taken and other information shall be gathered in a manner (e.g., using proper “chain of custody...

  1. Molecular characteristics and efficacy of 16D10 siRNAs in inhibiting root-knot nematode infection in transgenic grape hairy roots.

    PubMed

    Yang, Yingzhen; Jittayasothorn, Yingyos; Chronis, Demosthenis; Wang, Xiaohong; Cousins, Peter; Zhong, Gan-Yuan

    2013-01-01

    Root-knot nematodes (RKNs) infect many annual and perennial crops and are the most devastating soil-born pests in vineyards. To develop a biotech-based solution for controlling RKNs in grapes, we evaluated the efficacy of plant-derived RNA interference (RNAi) silencing of a conserved RKN effector gene, 16D10, for nematode resistance in transgenic grape hairy roots. Two hairpin-based silencing constructs, containing a stem sequence of 42 bp (pART27-42) or 271 bp (pART27-271) of the 16D10 gene, were transformed into grape hairy roots and compared for their small interfering RNA (siRNA) production and efficacy on suppression of nematode infection. Transgenic hairy root lines carrying either of the two RNAi constructs showed less susceptibility to nematode infection compared with control. Small RNA libraries from four pART27-42 and two pART27-271 hairy root lines were sequenced using an Illumina sequencing technology. The pART27-42 lines produced hundred times more 16D10-specific siRNAs than the pART27-271 lines. On average the 16D10 siRNA population had higher GC content than the 16D10 stem sequences in the RNAi constructs, supporting previous observation that plant dicer-like enzymes prefer GC-rich sequences as substrates for siRNA production. The stems of the 16D10 RNAi constructs were not equally processed into siRNAs. Several hot spots for siRNA production were found in similar positions of the hairpin stems in pART27-42 and pART27-271. Interestingly, stem sequences at the loop terminus produced more siRNAs than those at the stem base. Furthermore, the relative abundance of guide and passenger single-stranded RNAs from putative siRNA duplexes was largely correlated with their 5' end thermodynamic strength. This study demonstrated the feasibility of using a plant-derived RNAi approach for generation of novel nematode resistance in grapes and revealed several interesting molecular characteristics of transgene siRNAs important for optimizing plant RNAi constructs.

  2. Multi-institutional analysis of CT and MRI reports evaluating indeterminate renal masses: comparison to a national survey investigating desired report elements.

    PubMed

    Hu, Eric M; Zhang, Andrew; Silverman, Stuart G; Pedrosa, Ivan; Wang, Zhen J; Smith, Andrew D; Chandarana, Hersh; Doshi, Ankur; Shinagare, Atul B; Remer, Erick M; Kaffenberger, Samuel D; Miller, David C; Davenport, Matthew S

    2018-04-17

    To determine the need for a standardized renal mass reporting template by analyzing reports of indeterminate renal masses and comparing their contents to stated preferences of radiologists and urologists. The host IRB waived regulatory oversight for this multi-institutional HIPAA-compliant quality improvement effort. CT and MRI reports created to characterize an indeterminate renal mass were analyzed from 6 community (median: 17 reports/site) and 6 academic (median: 23 reports/site) United States practices. Report contents were compared to a published national survey of stated preferences by academic radiologists and urologists from 9 institutions. Descriptive statistics and Chi-square tests were calculated. Of 319 reports, 85% (271; 192 CT, 79 MRI) reported a possibly malignant mass (236 solid, 35 cystic). Some essential elements were commonly described: size (99% [269/271]), mass type (solid vs. cystic; 99% [268/271]), enhancement (presence vs. absence; 92% [248/271]). Other essential elements had incomplete penetrance: the presence or absence of fat in solid masses (14% [34/236]), size comparisons when available (79% [111/140]), Bosniak classification for cystic masses (54% [19/35]). Preferred but non-essential elements generally were described in less than half of reports. Nephrometry scores usually were not included for local therapy candidates (12% [30/257]). Academic practices were significantly more likely than community practices to include mass characterization details, probability of malignancy, and staging. Community practices were significantly more likely to include management recommendations. Renal mass reporting elements considered essential or preferred often are omitted in radiology reports. Variation exists across radiologists and practice settings. A standardized template may mitigate these inconsistencies.

  3. 77 FR 271 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    ... Threat Reduction Agency, 1680 Texas Street SE., Kirtland Air Force Base, Albuquerque, NM 87117-5669... Threat Reduction Agency, 1680 Texas Street SE., Kirtland Air Force Base, Albuquerque, NM 87117-5669...

  4. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Occupational Safety and Health Administration, U.S. Department of Labor, 200 Constitution Avenue, NW, Room... Administration, U.S. Department of Labor, John F. Kennedy Federal Building, Room E-340, Boston, Massachusetts...

  5. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., Occupational Safety and Health Administration, U.S. Department of Labor, 200 Constitution Avenue, NW, Room... Administration, U.S. Department of Labor, John F. Kennedy Federal Building, Room E-340, Boston, Massachusetts...

  6. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  7. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  8. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  9. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  10. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  11. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  12. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  13. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  14. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  15. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  16. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...-presidential electors, or the selection, nomination, or election of any individual to any Federal, State, or local elective public office, whether or not such individual or electors are selected, nominated, or elected. Accordingly, a political party includes a committee or other group which accepts contributions or...

  17. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...-presidential electors, or the selection, nomination, or election of any individual to any Federal, State, or local elective public office, whether or not such individual or electors are selected, nominated, or elected. Accordingly, a political party includes a committee or other group which accepts contributions or...

  18. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...-presidential electors, or the selection, nomination, or election of any individual to any Federal, State, or local elective public office, whether or not such individual or electors are selected, nominated, or elected. Accordingly, a political party includes a committee or other group which accepts contributions or...

  19. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...-presidential electors, or the selection, nomination, or election of any individual to any Federal, State, or local elective public office, whether or not such individual or electors are selected, nominated, or elected. Accordingly, a political party includes a committee or other group which accepts contributions or...

  20. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...-presidential electors, or the selection, nomination, or election of any individual to any Federal, State, or local elective public office, whether or not such individual or electors are selected, nominated, or elected. Accordingly, a political party includes a committee or other group which accepts contributions or...

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