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Sample records for darmstadtium 271

  1. Atomic Mass and NuclearBinding Energy for Ds-351(Darmstadtium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Ds-351 (Darmstadtium, atomic number Z = 110, mass number A = 351).

  2. 32. LOOKING NORTHEAST DOWN WALKWAY CONNECTING BUILDING NO.S 271, 271G, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. LOOKING NORTHEAST DOWN WALKWAY CONNECTING BUILDING NO.S 271, 271-G, 271-I, 271-L, 271-K, ETC. MIRRORS IN UPPER RIGHT PERMIT WORKERS TO SEE AROUND CORNER TO CORRIDOR LEADING TO BUILDING NO. 271-H (LEAD AZIDE PREPARATION BUILDING). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  3. 30. BUILDING NO.S 271K AND 271L, VIEW LOOKING SOUTH AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. BUILDING NO.S 271-K AND 271-L, VIEW LOOKING SOUTH AT BACK OF BUILDING NO. 271-L (LEFT), 271-K (MIDDLE) AND ROOF OF BUILDING NO. 271-I (VISIBLE OVER WALKWAY ON RIGHT). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  4. 48 CFR 1505.271 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true 1505.271 Section 1505.271 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY ACQUISITION PLANNING PUBLICIZING CONTRACT ACTIONS Synopses of Proposed Contract Actions...

  5. 48 CFR 1505.271 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false 1505.271 Section 1505.271 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY ACQUISITION PLANNING PUBLICIZING CONTRACT ACTIONS Synopses of Proposed Contract Actions...

  6. 48 CFR 1505.271 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false 1505.271 Section 1505.271 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY ACQUISITION PLANNING PUBLICIZING CONTRACT ACTIONS Synopses of Proposed Contract Actions...

  7. 48 CFR 1516.405-271 - Limitations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Limitations. 1516.405-271 Section 1516.405-271 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1516.405-271 Limitations. (a) No...

  8. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false General objectives. 27.1 Section 27.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR CAPE COD NATIONAL SEASHORE; ZONING STANDARDS § 27.1 General objectives. (a) Consistent with the...

  9. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Carrier costs. 271.4 Section 271.4 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.4 Carrier costs. (a) The reasonable costs...

  10. 48 CFR 247.271-1 - Policy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Policy. 247.271-1 Section 247.271-1 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT TRANSPORTATION Contracts for Transportation or for Transportation-Related Services 247.271-1 Policy. (a)...

  11. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Treatment program. 264.271 Section 264.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a)...

  12. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... of 31 U.S.C. 751. For purposes of the regulations in this part and 4 CFR part 28, a simple majority... 4 Accounts 1 2011-01-01 2011-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the...

  13. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... of 31 U.S.C. 751. For purposes of the regulations in this part and 4 CFR part 28, a simple majority... 4 Accounts 1 2013-01-01 2013-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the...

  14. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... of 31 U.S.C. 751. For purposes of the regulations in this part and 4 CFR part 28, a simple majority... 4 Accounts 1 2014-01-01 2013-01-01 true The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the...

  15. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... of 31 U.S.C. 751. For purposes of the regulations in this part and 4 CFR part 28, a simple majority... 4 Accounts 1 2012-01-01 2012-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the...

  16. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of 31 U.S.C. 751. For purposes of the regulations in this part and 4 CFR part 28, a simple majority... 4 Accounts 1 2010-01-01 2010-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the...

  17. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of...

  18. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of...

  19. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of...

  20. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of...

  1. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of...

  2. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Definitions. 271.2 Section 271.2 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.2 Definitions. Access device means any card, plate, code,...

  3. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 4 2012-01-01 2012-01-01 false Definitions. 271.2 Section 271.2 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.2 Definitions. Access device means any card, plate, code,...

  4. 48 CFR 1516.405-271 - Limitations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Limitations. 1516.405-271 Section 1516.405-271 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1516.405-271 Limitations. (a) No award fee may be earned if the Fee...

  5. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Published information. 271.3 Section 271.3 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the Federal Register, in addition to this...

  6. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Published information. 271.3 Section 271.3... AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the... the actions, and the votes taken. (c) Other published information. From time to time,...

  7. 14 CFR 27.1 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Applicability. 27.1 Section 27.1 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... standards for the issue of type certificates, and changes to those certificates, for normal...

  8. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the...

  9. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the...

  10. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the...

  11. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the...

  12. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the...

  13. 12 CFR 271.8 - Subpoenas.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Subpoenas. 271.8 Section 271.8 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF... employee of the Committee, of the Board of Governors of the Federal Reserve System, or of a Federal...

  14. 40 CFR 271.20 - Approval process.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Approval process. 271.20 Section 271.20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... Approval process. (a) Prior to submitting an application to EPA for approval of a State program, the...

  15. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Carrier revenues. 271.5 Section 271.5... revenues. (a) The projected passenger revenue for a carrier providing essential air service at an eligible... reasonableness of a carrier's passenger revenue projections will be evaluated by: (1) Comparing the...

  16. 7 CFR 1150.271 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Definitions. 1150.271 Section 1150.271 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Department's Agricultural Marketing Service; (e) Producer means any person engaged in the production of...

  17. 7 CFR 1150.271 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Definitions. 1150.271 Section 1150.271 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Department's Agricultural Marketing Service; (e) Producer means any person engaged in the production of...

  18. 7 CFR 1150.271 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Definitions. 1150.271 Section 1150.271 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Department's Agricultural Marketing Service; (e) Producer means any person engaged in the production of...

  19. 40 CFR 271.20 - Approval process.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Approval process. 271.20 Section 271.20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... Approval process. (a) Prior to submitting an application to EPA for approval of a State program, the...

  20. 38 CFR 17.271 - Eligibility.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2013-07-01 2013-07-01 false Eligibility. 17.271 Section 17.271 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Civilian Health and Medical Program of the Department of Veterans Affairs (champva)-Medical Care for Survivors and Dependents of Certain Veterans §...

  1. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish...

  2. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish...

  3. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish...

  4. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish...

  5. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2012-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following clause: Title (APR 2010) (a) Title to all materials and... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Title. 1352.271-80...

  6. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2015-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following clause: Title (APR 2010) (a) Title to all materials and... 48 Federal Acquisition Regulations System 5 2015-10-01 2015-10-01 false Title. 1352.271-80...

  7. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Definitions. 271.2 Section 271.2 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF... results of which are not intended to promote any particular product or industry. (i) Records of...

  8. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Installation requirements. 171.271 Section 171.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System...

  9. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2014-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following clause: Title (APR 2010) (a) Title to all materials and... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Title. 1352.271-80...

  10. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible...

  11. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible...

  12. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible...

  13. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible...

  14. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible...

  15. 12 CFR 271.6 - Processing requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AVAILABILITY OF INFORMATION § 271.6 Processing requests. (a) Receipt of requests. The date of receipt for any... reasonably be expected to pose an imminent threat to the life or physical safety of an individual; or...

  16. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... COD NATIONAL SEASHORE; ZONING STANDARDS § 27.1 General objectives. (a) Consistent with the objectives... Cape Cod National Seashore will be conducted in a manner which will assure the widest possible public... Cape Cod National Seashore and situated in the towns of Provincetown, Truro, Wellfleet,...

  17. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... COD NATIONAL SEASHORE; ZONING STANDARDS § 27.1 General objectives. (a) Consistent with the objectives... Cape Cod National Seashore will be conducted in a manner which will assure the widest possible public... Cape Cod National Seashore and situated in the towns of Provincetown, Truro, Wellfleet,...

  18. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... COD NATIONAL SEASHORE; ZONING STANDARDS § 27.1 General objectives. (a) Consistent with the objectives... Cape Cod National Seashore will be conducted in a manner which will assure the widest possible public... Cape Cod National Seashore and situated in the towns of Provincetown, Truro, Wellfleet,...

  19. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission...

  20. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission...

  1. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission...

  2. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... policy issues regarding the application of exemptions. (l)(1) Search means a reasonable search, by manual... requested. For purposes of computing fees under § 271.9, search time includes all time spent looking for... from disclosure. (2) Search does not mean or include research, creation of any document, or...

  3. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... policy issues regarding the application of exemptions. (l)(1) Search means a reasonable search, by manual... requested. For purposes of computing fees under § 271.9, search time includes all time spent looking for... from disclosure. (2) Search does not mean or include research, creation of any document, or...

  4. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... device requirements of 40 CFR 60.18. (3) The specifications and requirements listed in paragraphs (c)(1...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage...

  5. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... device requirements of 40 CFR 60.18. (3) The specifications and requirements listed in paragraphs (c)(1...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage...

  6. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... device requirements of 40 CFR 60.18. (3) The specifications and requirements listed in paragraphs (c)(1...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage...

  7. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... device requirements of 40 CFR 60.18. (3) The specifications and requirements listed in paragraphs (c)(1...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage...

  8. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... device requirements of 40 CFR 60.18. (3) The specifications and requirements listed in paragraphs (c)(1...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage...

  9. 14 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.2 Definitions...) Received scheduled air transportation at any time between January 1, 1990, and November 4, 1990; and (3) Is... compensation under Subchapter II of Chapter 417 of the Statute. Essential air service is that...

  10. 14 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.2 Definitions...) Received scheduled air transportation at any time between January 1, 1990, and November 4, 1990; and (3) Is... compensation under Subchapter II of Chapter 417 of the Statute. Essential air service is that...

  11. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271... by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  12. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271... by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  13. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271... by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  14. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271... by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  15. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271... by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  16. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... Section 271.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF...

  17. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... Section 271.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF...

  18. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission...

  19. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission...

  20. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Performance....

  1. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 49 CFR part 21; and (3) The Rehabilitation Act of 1973, 49 CFR part 27, and part 382 of this chapter... assurances required by 49 CFR parts 20, 21, 27 and 29, and § 382.21 of this chapter. ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Discrimination prohibited. 271.9...

  2. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 49 CFR part 21; and (3) The Rehabilitation Act of 1973, 49 CFR part 27, and part 382 of this chapter... assurances required by 49 CFR parts 20, 21, 27 and 29, and § 382.21 of this chapter. ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Discrimination prohibited. 271.9...

  3. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 49 CFR part 21; and (3) The Rehabilitation Act of 1973, 49 CFR part 27, and part 382 of this chapter... assurances required by 49 CFR parts 20, 21, 27 and 29, and § 382.21 of this chapter. ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Discrimination prohibited. 271.9...

  4. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 49 CFR part 21; and (3) The Rehabilitation Act of 1973, 49 CFR part 27, and part 382 of this chapter... assurances required by 49 CFR parts 20, 21, 27 and 29, and § 382.21 of this chapter. ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Discrimination prohibited. 271.9...

  5. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 49 CFR part 21; and (3) The Rehabilitation Act of 1973, 49 CFR part 27, and part 382 of this chapter... assurances required by 49 CFR parts 20, 21, 27 and 29, and § 382.21 of this chapter. ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Discrimination prohibited. 271.9...

  6. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... used under title XVI of the Social Security Act (as set forth at 20 CFR part 416, subpart I...: For Federal Register citations affecting § 271.2, see the List of CFR Sections Affected, which appears... composition. In order to develop maximum food stamp allotments, the Secretary shall make household size...

  7. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... forth at 20 CFR part 416, subpart I, Determining Disability and Blindness as defined in Title XVI...: For Federal Register citations affecting § 271.2, see the List of CFR Sections Affected, which appears... composition. In order to develop maximum food stamp allotments, the Secretary shall make household size...

  8. 48 CFR 719.271-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-1 General. The purpose of this section is to prescribe responsibilities and procedures for carrying out the small business program policy set forth in 219.270, and in part 19 of the FAR. Small Business concerns are defined in FAR subpart 19.1; in addition,...

  9. 48 CFR 719.271 - Agency program direction and operation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Agency program direction and operation. 719.271 Section 719.271 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271 Agency program direction...

  10. 48 CFR 719.271 - Agency program direction and operation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Agency program direction and operation. 719.271 Section 719.271 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271 Agency program direction...

  11. 12 CFR 27.1 - Scope and OMB control number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...-permanent financing, or refinancing of residential real property. (b) OMB control number. The collection of... 12 Banks and Banking 1 2011-01-01 2011-01-01 false Scope and OMB control number. 27.1 Section 27.1... SYSTEM § 27.1 Scope and OMB control number. (a) Scope. This part applies to the activities of...

  12. 12 CFR 27.1 - Scope and OMB control number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-permanent financing, or refinancing of residential real property. (b) OMB control number. The collection of... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Scope and OMB control number. 27.1 Section 27.1... SYSTEM § 27.1 Scope and OMB control number. (a) Scope. This part applies to the activities of...

  13. 12 CFR 271.1 - Authority and purpose.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Authority and purpose. 271.1 Section 271.1 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.1 Authority and purpose. (a) Authority. This part is issued by the...

  14. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Power to revoke. 271.7 Section 271.7 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.7 Power to revoke. It is the intention of the regulations in this part that the...

  15. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education...

  16. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education...

  17. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education...

  18. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education...

  19. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of increasing public information regarding forest fire prevention. ... materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.2 Use of official campaign materials. Official Cooperative...

  20. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of increasing public information regarding forest fire prevention. ... materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.2 Use of official campaign materials. Official Cooperative...

  1. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  2. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  3. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  4. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  5. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Power to revoke. 271.7 Section 271.7 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.7 Power to revoke. It is the intention of the regulations in this part that the...

  6. 40 CFR 271.17 - Sharing of information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Sharing of information. 271.17 Section 271.17 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.17 Sharing of information. (a)...

  7. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education...

  8. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Allotment reduction procedures. 271.7 Section 271.7 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7 Allotment reduction procedures. (a)...

  9. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program...

  10. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program...

  11. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program...

  12. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program...

  13. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program...

  14. CD271 is an imperfect marker for melanoma initiating cells

    PubMed Central

    Cheli, Yann; Bonnazi, Vanessa F.; Jacquel, Arnaud; Allegra, Maryline; Donatis, Gian Marco De; Bahadoran, Philippe; Bertolotto, Corine; Ballotti, Robert

    2014-01-01

    Understanding the molecular and cellular processes underlying melanoma plasticity and heterogeneity is of paramount importance to improve the efficiency of current treatment and to overcome resistance to chemotherapy drugs. The notion of plasticity and heterogeneity implies the existence of melanoma cell populations with different phenotypic and tumorigenic properties. Using melanoma cell lines and melanoma cells freshly isolated from patient biopsies, we investigated the relationship between ABCB5+, CD271+ and low-MITF, expressing populations that were reported to display melanoma initiating cell properties. Here, we showed that ABCB5+ and CD271+ populations poorly overlap. However, we found that the CD271+ population is enriched in low-MITF cells and expresses a higher level of stemness genes, such as OCT4, NANOG and NES. These features could explain the increased tumorigenicity of the CD271+ cells. The rapid conversion of CD271+ to CD271− cells in vitro demonstrates the plasticity ability of melanoma cells. Finally, we observed that the transient slow-growing population contains only CD271+ cells that are highly tumorigenic. However, the fast growing/CD271+ population exhibits a poor tumorigenic ability. Taking together, our data show that CD271 is an imperfect marker for melanoma initiating cells, but may be useful to identify melanoma cells with an increased stemness and tumorigenic potential. PMID:25105565

  15. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES...

  16. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES...

  17. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES...

  18. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES...

  19. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES...

  20. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production...

  1. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production...

  2. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production...

  3. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production...

  4. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production...

  5. 14 CFR 271.7 - Subsidy payout formula.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Subsidy payout formula. 271.7 Section 271.7 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... payout formula. (a) Subsidy will be paid by the Department to the air carrier monthly, based on...

  6. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Base fee. 1816.405-271... CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1816.405-271 Base fee. (a) A base fee shall not be used on CPAF contracts for which the periodic award fee evaluations are...

  7. 7 CFR 271.3 - Delegations to FNS for administration.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 4 2014-01-01 2014-01-01 false Delegations to FNS for administration. 271.3 Section 271.3 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS §...

  8. 7 CFR 271.3 - Delegations to FNS for administration.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 4 2012-01-01 2012-01-01 false Delegations to FNS for administration. 271.3 Section 271.3 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS §...

  9. 7 CFR 271.3 - Delegations to FNS for administration.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 4 2011-01-01 2011-01-01 false Delegations to FNS for administration. 271.3 Section 271.3 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS §...

  10. 7 CFR 271.3 - Delegations to FNS for administration.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 4 2013-01-01 2013-01-01 false Delegations to FNS for administration. 271.3 Section 271.3 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS §...

  11. 7 CFR 271.3 - Delegations to FNS for administration.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Delegations to FNS for administration. 271.3 Section 271.3 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS §...

  12. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of...

  13. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of...

  14. 27 CFR 19.271 - Construction of buildings

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Construction of buildings 19.271 Section 19.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU... Construction of buildings Buildings in which spirits, denatured spirits, articles, or wines are...

  15. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE MAMMALS Taking Marine...

  16. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE MAMMALS Taking Marine...

  17. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE MAMMALS Taking Marine...

  18. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE MAMMALS Taking Marine...

  19. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI......

  20. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI......

  1. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI......

  2. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI......

  3. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI......

  4. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... in accordance with the procedures of 40 CFR part 22 in the case of a violation of a State program... 40 Protection of Environment 26 2010-07-01 2010-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on...

  5. 29 CFR 500.271 - Certification of official record.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Certification of official record. 500.271 Section 500.271 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Record §...

  6. 29 CFR 500.271 - Certification of official record.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Certification of official record. 500.271 Section 500.271 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Record §...

  7. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Facility pest management practice standard. 205.271 Section 205.271 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS...

  8. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Facility pest management practice standard. 205.271 Section 205.271 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS...

  9. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations §...

  10. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations §...

  11. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations §...

  12. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and...

  13. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and...

  14. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and...

  15. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and...

  16. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... from the Production of Methyl Bromide 54 FR 41402-408 Apr. 6, 1990. Dec. 11, 1989 Listing Certain... affecting § 271.1, see the List of CFR Sections Affected, which appears in the Finding Aids section of...

  17. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... from the Production of Methyl Bromide 54 FR 41402-408 Apr. 6, 1990. Dec. 11, 1989 Listing Certain... affecting § 271.1, see the List of CFR Sections Affected, which appears in the Finding Aids section of...

  18. Interior view showing interface between building 271 and with building ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view showing interface between building 271 and with building 87 in background; camera facing west. - Mare Island Naval Shipyard, Mechanics Shop, Waterfront Avenue, west side between A Street & Third Street, Vallejo, Solano County, CA

  19. Atomic Mass and Nuclear Binding Energy for Pa-271 (Protactinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Pa-271 (Protactinium, atomic number Z = 91, mass number A = 271).

  20. Atomic Mass and Nuclear Binding Energy for Uut-271 (Ununtrium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Uut-271 (Ununtrium, atomic number Z = 113, mass number A = 271).

  1. Atomic Mass and Nuclear Binding Energy for Sg-271 (Seaborgium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Sg-271 (Seaborgium, atomic number Z = 106, mass number A = 271).

  2. Atomic Mass and Nuclear Binding Energy for Bh-271 (Bohrium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Bh-271 (Bohrium, atomic number Z = 107, mass number A = 271).

  3. Atomic Mass and Nuclear Binding Energy for Mt-271 (Meitnerium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Mt-271 (Meitnerium, atomic number Z = 109, mass number A = 271).

  4. Atomic Mass and Nuclear Binding Energy for Es-271 (Einsteinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Es-271 (Einsteinium, atomic number Z = 99, mass number A = 271).

  5. Atomic Mass and Nuclear Binding Energy for At-271 (Astatine)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope At-271 (Astatine, atomic number Z = 85, mass number A = 271).

  6. Atomic Mass and Nuclear Binding Energy for Ac-271 (Actinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Ac-271 (Actinium, atomic number Z = 89, mass number A = 271).

  7. Atomic Mass and Nuclear Binding Energy for Po-271 (Polonium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Po-271 (Polonium, atomic number Z = 84, mass number A = 271).

  8. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a... a commercial permit for Atlantic dolphin and wahoo has been issued, as required under § 622.270(a)(1), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining...

  9. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a... a commercial permit for Atlantic dolphin and wahoo has been issued, as required under § 622.270(a)(1), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining...

  10. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Fire-extinguishing system materials. 121....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each component of a fire-extinguishing system that is in a designated fire zone must be made of...

  11. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review...

  12. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-84 Access to the vessel. As prescribed in 48 CFR 1371.115, insert the following clause: Access to the Vessel (APR... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Access to the vessel....

  13. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Construction 536.271 Project labor agreements. (a) Authority. This subpart implements the Presidential memorandum of June 5, 1997, on using project labor agreements (PLAs) on Federal construction projects. The... significant project means a Federal construction project with a total cost to the Federal Government of...

  14. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening... certifies in writing that the public exigency will not permit the delay incident to screening...

  15. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of work. As prescribed in 48 CFR 1371.104, insert the following clause. Schedule of Work (APR 2010... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Schedule of work. 1352.271... arrival at the contractor's facility: (1) Production Schedule. (2) Work Package Network. (3)...

  16. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of work. As prescribed in 48 CFR 1371.104, insert the following clause. Schedule of Work (APR 2010... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Schedule of work. 1352.271... arrival at the contractor's facility: (1) Production Schedule. (2) Work Package Network. (3)...

  17. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  18. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  19. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  20. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  1. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  2. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  3. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  4. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  5. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... Section 271.7 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY... fostering public information in the prevention of forest fires. All authorities and licenses granted...

  6. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-3 USAID contracting officers. With...) Being thoroughly familiar with part 19 of the FAR and this section dealing with the small business... procurements; (c) Assuring that small business concerns and minority business enterprises are...

  7. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities. In order for the agency small business program to be effective, the active support of top management... made by SDB; (b) Consulting with SDB in establishing small business and minority business...

  8. 12 CFR 271.9 - Fee schedules; waiver of fees.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 271.9 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES..., duplication, and review apply when records are requested for commercial use. (2) Educational, research, or..., whose purpose is scholarly or scientific research. The first 100 pages of duplication, however, will...

  9. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  10. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  11. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  12. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  13. Contextual view of building 271 from across the river shown ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view of building 271 from across the river shown at center between coal sheds at left and building 91 at right; camera facing northwest. - Mare Island Naval Shipyard, Mechanics Shop, Waterfront Avenue, west side between A Street & Third Street, Vallejo, Solano County, CA

  14. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... maximum food stamp allotments for each household size and the computer program shall be adjusted to allow for the minimum benefit for one- and two-person households. The computer program shall also be... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7...

  15. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... computer program shall also be adjusted to provide for the rounding of benefit levels of $1, $3 and $5 to... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7 Allotment... best available data pertaining to the number of people participating in the program and the amounts...

  16. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... maximum food stamp allotments for each household size and the computer program shall be adjusted to allow for the minimum benefit for one- and two-person households. The computer program shall also be... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7...

  17. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... maximum food stamp allotments for each household size and the computer program shall be adjusted to allow for the minimum benefit for one- and two-person households. The computer program shall also be... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7...

  18. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic Production and Handling Requirements § 205.271 Facility pest management practice standard. (a) The producer or handler of an organic... habitat, food sources, and breeding areas; (2) Prevention of access to handling facilities; and...

  19. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic Production and Handling Requirements § 205.271 Facility pest management practice standard. (a) The producer or handler of an organic... habitat, food sources, and breeding areas; (2) Prevention of access to handling facilities; and...

  20. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review...

  1. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review...

  2. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review...

  3. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... spectrum is made available and licensed for the provision of wireless communications services in the... user, the added text is set forth as follows: § 27.1 Basis and purpose. (b) * * * (14) Spectrum in the... pursuant to section 6403 of the Spectrum Act. The specific frequencies and number of channel blocks will...

  4. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  5. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  6. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  7. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  8. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  9. 48 CFR 1352.271-78 - Minimization of delay due to Government furnished property.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Provisions and Clauses 1352.271-78 Minimization of delay due to Government furnished property. As prescribed in 48 CFR 1371.109, insert the following clause: Minimization of Delay Due to Government Furnished... to Government furnished property. 1352.271-78 Section 1352.271-78 Federal Acquisition...

  10. 48 CFR 1352.271-78 - Minimization of delay due to Government furnished property.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Provisions and Clauses 1352.271-78 Minimization of delay due to Government furnished property. As prescribed in 48 CFR 1371.109, insert the following clause: Minimization of Delay Due to Government Furnished... to Government furnished property. 1352.271-78 Section 1352.271-78 Federal Acquisition...

  11. 48 CFR 1352.271-78 - Minimization of delay due to Government furnished property.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Provisions and Clauses 1352.271-78 Minimization of delay due to Government furnished property. As prescribed in 48 CFR 1371.109, insert the following clause: Minimization of Delay Due to Government Furnished... to Government furnished property. 1352.271-78 Section 1352.271-78 Federal Acquisition...

  12. 48 CFR 1352.271-78 - Minimization of delay due to Government furnished property.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Provisions and Clauses 1352.271-78 Minimization of delay due to Government furnished property. As prescribed in 48 CFR 1371.109, insert the following clause: Minimization of Delay Due to Government Furnished... to Government furnished property. 1352.271-78 Section 1352.271-78 Federal Acquisition...

  13. 48 CFR 1352.271-78 - Minimization of delay due to Government furnished property.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Provisions and Clauses 1352.271-78 Minimization of delay due to Government furnished property. As prescribed in 48 CFR 1371.109, insert the following clause: Minimization of Delay Due to Government Furnished... to Government furnished property. 1352.271-78 Section 1352.271-78 Federal Acquisition...

  14. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... evacuation service (HEMES). 135.271 Section 135.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... 24-consecutive hour period of a HEMES assignment, unless an emergency medical evacuation operation...

  15. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... evacuation service (HEMES). 135.271 Section 135.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... 24-consecutive hour period of a HEMES assignment, unless an emergency medical evacuation operation...

  16. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... evacuation service (HEMES). 135.271 Section 135.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... 24-consecutive hour period of a HEMES assignment, unless an emergency medical evacuation operation...

  17. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... evacuation service (HEMES). 135.271 Section 135.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... 24-consecutive hour period of a HEMES assignment, unless an emergency medical evacuation operation...

  18. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  19. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  20. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  1. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  2. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  3. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... actions that are exempted from screening. 719.271-7 Section 719.271-7 Federal Acquisition Regulations....271-7 Reports on procurement actions that are exempted from screening. (a) Unilateral and class set... procurement from screening. The determination shall cite the pertinent facts which led to his/her...

  4. Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation.

    PubMed

    Calabrese, Giovanna; Giuffrida, Raffaella; Lo Furno, Debora; Parrinello, Nunziatina Laura; Forte, Stefano; Gulino, Rosario; Colarossi, Cristina; Schinocca, Luciana Rita; Giuffrida, Rosario; Cardile, Venera; Memeo, Lorenzo

    2015-01-01

    The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271- mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate. PMID:26184166

  5. 46 CFR 502.271 - Special docket application for permission to refund or waive freight charges.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 9 2011-10-01 2011-10-01 false Special docket application for permission to refund or waive freight charges. 502.271 Section 502.271 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS RULES OF PRACTICE AND PROCEDURE Refund or Waiver of Freight Charges § 502.271 Special docket application for permission to...

  6. 48 CFR 1352.271-74 - Foreseeable cost factors pertaining to different shipyard locations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Provisions and Clauses 1352.271-74 Foreseeable cost factors pertaining to different shipyard locations. As prescribed in 48 CFR 1371.105, insert the following provision: Foreseeable Cost Factors Pertaining to... pertaining to different shipyard locations. 1352.271-74 Section 1352.271-74 Federal Acquisition...

  7. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that...

  8. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none...

  9. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... evacuation service (HEMES). 135.271 Section 135.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... 24-consecutive hour period of a HEMES assignment, unless an emergency medical evacuation operation...

  10. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Additional Item Requirements (AIR)-growth work 1352.271-72 Section 1352.271-72 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-72 Additional Item...

  11. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Additional Item Requirements (AIR)-growth work 1352.271-72 Section 1352.271-72 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-72 Additional Item...

  12. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  13. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  14. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  15. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation...

  16. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation...

  17. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation...

  18. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation...

  19. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation...

  20. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... Inspections and Tests § 234.271 Insulated rail joints, bond wires, and track connections. Insulated...

  1. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... Inspections and Tests § 234.271 Insulated rail joints, bond wires, and track connections. Insulated...

  2. 48 CFR 1352.271-74 - Foreseeable cost factors pertaining to different shipyard locations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Provisions and Clauses 1352.271-74 Foreseeable cost factors pertaining to different shipyard locations. As prescribed in 48 CFR 1371.105, insert the following provision: Foreseeable Cost Factors Pertaining to... pertaining to different shipyard locations. 1352.271-74 Section 1352.271-74 Federal Acquisition...

  3. 48 CFR 1352.271-71 - Method of payment and invoicing instructions for ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Provisions and Clauses 1352.271-71 Method of payment and invoicing instructions for ship repair. As prescribed in 48 CFR 1371.102, insert the following clause: Method of Payment and Invoicing Instructions for... invoicing instructions for ship repair. 1352.271-71 Section 1352.271-71 Federal Acquisition...

  4. 48 CFR 1352.271-85 - Documentation of requests for equitable adjustment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Documentation of requests for equitable adjustment. 1352.271-85 Section 1352.271-85 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-85 Documentation...

  5. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells.

    PubMed

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-01-01

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells' tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC. PMID:27469492

  6. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells

    PubMed Central

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-01-01

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells’ tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC. PMID:27469492

  7. CD271 Expression on Patient Melanoma Cells Is Unstable and Unlinked to Tumorigenicity.

    PubMed

    Boyle, Samantha E; Fedele, Clare G; Corbin, Vincent; Wybacz, Elisha; Szeto, Pacman; Lewin, Jeremy; Young, Richard J; Wong, Annie; Fuller, Robert; Spillane, John; Speakman, David; Donahoe, Simon; Pohl, Miklos; Gyorki, David; Henderson, Michael A; Johnstone, Ricky W; Papenfuss, Anthony T; Shackleton, Mark

    2016-07-01

    The stability of markers that identify cancer cells that propagate disease is important to the outcomes of targeted therapy strategies. In human melanoma, conflicting data exist as to whether hierarchical expression of CD271/p75/NGFR (nerve growth factor receptor) marks cells with enriched tumorigenicity, which would compel their specific targeting in therapy. To test whether these discrepancies relate to differences among groups in assay approaches, we undertook side-by-side testing of published methods of patient-derived melanoma xenografting (PDX), including comparisons of tissue digestion procedures or coinjected Matrigel formulations. We found that CD271(-) and CD271(+) melanoma cells from each of seven patients were similarly tumorigenic, regardless of assay variations. Surprisingly variable CD271 expression patterns were observed in the analyses of sibling PDX tumors (n = 68) grown in the same experiments from either CD271(-) or CD271(+) cells obtained from patients. This indicates unstable intratumoral lineage relationships between CD271(-) and CD271(+) melanoma cells that are inconsistent with classical, epigenetically based theories of disease progression, such as the cancer stem cell and plasticity models. SNP genotyping of pairs of sibling PDX tumors grown from phenotypically identical CD271(-) or CD271(+) cells showed large pairwise differences in copy number (28%-48%). Differences were also apparent in the copy number profiles of CD271(-) and CD271(+) cells purified directly from each of the four melanomas (1.4%-23%). Thus, CD271 expression in patient melanomas is unstable, not consistently linked to increased tumorigenicity and associated with genetic heterogeneity, undermining its use as a marker in clinical studies. Cancer Res; 76(13); 3965-77. ©2016 AACR. PMID:27325642

  8. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Requirements for hazardous waste management facilities. 271.12 Section 271.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization §...

  9. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for transporters of hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization §...

  10. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for hazardous waste management facilities. 271.12 Section 271.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization §...

  11. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Requirements for transporters of hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization §...

  12. 42 CFR 447.271 - Upper limits based on customary charges.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Upper limits based on customary charges. 447.271 Section 447.271 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS PAYMENTS FOR SERVICES Payment for Inpatient Hospital and Long-Term Care Facility Services...

  13. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing problems corrected? (a) As a BAT or STT, you have the responsibility of trying to complete successfully...

  14. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET...'s Freedom of Information Office pursuant to 5 U.S.C. 552(a)(2). (b) Reading room procedures. (1.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve...

  15. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 3 2011-04-01 2011-04-01 false Debts owed by political parties. 1.271-1 Section... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.271-1 Debts owed by political parties. (a... arose, owed by a political party. For example, it is immaterial that the debt may have arisen as...

  16. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 3 2014-04-01 2014-04-01 false Debts owed by political parties. 1.271-1 Section... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.271-1 Debts owed by political parties. (a... arose, owed by a political party. For example, it is immaterial that the debt may have arisen as...

  17. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 3 2012-04-01 2012-04-01 false Debts owed by political parties. 1.271-1 Section... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.271-1 Debts owed by political parties. (a... arose, owed by a political party. For example, it is immaterial that the debt may have arisen as...

  18. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 3 2013-04-01 2013-04-01 false Debts owed by political parties. 1.271-1 Section... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.271-1 Debts owed by political parties. (a... arose, owed by a political party. For example, it is immaterial that the debt may have arisen as...

  19. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware...

  20. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware...

  1. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Debts owed by political parties. 1.271-1 Section... (CONTINUED) INCOME TAXES Items Not Deductible § 1.271-1 Debts owed by political parties. (a) General rule. In... arose, owed by a political party. For example, it is immaterial that the debt may have arisen as...

  2. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware...

  3. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs....

  4. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs....

  5. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs....

  6. 7 CFR 271.4 - Delegations to State agencies for administration.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 4 2012-01-01 2012-01-01 false Delegations to State agencies for administration. 271.4 Section 271.4 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL...

  7. 7 CFR 271.4 - Delegations to State agencies for administration.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 4 2013-01-01 2013-01-01 false Delegations to State agencies for administration. 271.4 Section 271.4 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL...

  8. 7 CFR 271.4 - Delegations to State agencies for administration.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Delegations to State agencies for administration. 271.4 Section 271.4 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL...

  9. 7 CFR 271.4 - Delegations to State agencies for administration.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 4 2011-01-01 2011-01-01 false Delegations to State agencies for administration. 271.4 Section 271.4 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL...

  10. 7 CFR 271.4 - Delegations to State agencies for administration.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 4 2014-01-01 2014-01-01 false Delegations to State agencies for administration. 271.4 Section 271.4 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL...

  11. 34 CFR 271.10 - What types of projects may be funded?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false What types of projects may be funded? 271.10 Section 271.10 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION STATE EDUCATIONAL AGENCY DESEGREGATION PROGRAM What Kinds of Activities Does the Secretary Assist...

  12. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... business enterprises; (7) Establishing small business class set-aside for types and classes of items of... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS...

  13. 48 CFR 212.271 - Limitation on acquisition of right-hand drive passenger sedans.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of right-hand drive passenger sedans. 212.271 Section 212.271 Federal Acquisition Regulations System... acquisition of right-hand drive passenger sedans. 10 U.S.C. 2253(a)(2) limits the authority to purchase right-hand drive passenger sedans to a cost of not more than $40,000 per vehicle....

  14. 48 CFR 212.271 - Limitation on acquisition of right-hand drive passenger sedans.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of right-hand drive passenger sedans. 212.271 Section 212.271 Federal Acquisition Regulations System... acquisition of right-hand drive passenger sedans. 10 U.S.C. 2253(a)(2) limits the authority to purchase right-hand drive passenger sedans to a cost of not more than $40,000 per vehicle....

  15. 48 CFR 212.271 - Limitation on acquisition of right-hand drive passenger sedans.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... of right-hand drive passenger sedans. 212.271 Section 212.271 Federal Acquisition Regulations System... acquisition of right-hand drive passenger sedans. 10 U.S.C. 2253(a)(2) limits the authority to purchase right-hand drive passenger sedans to a cost of not more than $40,000 per vehicle....

  16. 30 CFR 250.271 - For what reasons will MMS disapprove the DPP or DOCD?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false For what reasons will MMS disapprove the DPP or DOCD? 250.271 Section 250.271 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR... State has not yet issued a final decision on your coastal zone consistency certification (see 15 CFR...

  17. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  18. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior INDIAN... tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  19. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  20. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  1. 27 CFR 70.271 - Procedure in the case of transferred assets.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Procedure in the case of transferred assets. 70.271 Section 70.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND... transferred assets. (a) Method of collection. (1) The liability, at law or in equity, of a transferee...

  2. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  3. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET...'s Freedom of Information Office pursuant to 5 U.S.C. 552(a)(2). (b) Reading room procedures. (1.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve...

  4. 48 CFR 247.271 - Contracts for the preparation of personal property for shipment or storage.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Contracts for the preparation of personal property for shipment or storage. 247.271 Section 247.271 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT...

  5. 48 CFR 247.271 - Contracts for the preparation of personal property for shipment or storage.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Contracts for the preparation of personal property for shipment or storage. 247.271 Section 247.271 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT...

  6. 48 CFR 247.271 - Contracts for the preparation of personal property for shipment or storage.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Contracts for the preparation of personal property for shipment or storage. 247.271 Section 247.271 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT...

  7. 48 CFR 247.271 - Contracts for the preparation of personal property for shipment or storage.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contracts for the preparation of personal property for shipment or storage. 247.271 Section 247.271 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT...

  8. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED... employee: (i) Has claimed or attempted to claim compensation under this Act; or (ii) has testified or...

  9. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED... employee: (i) Has claimed or attempted to claim compensation under this Act; or (ii) has testified or...

  10. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Discrimination; against employees who bring proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES ADMINISTRATION AND...

  11. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED... employee: (i) Has claimed or attempted to claim compensation under this Act; or (ii) has testified or...

  12. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards for ship repair. As prescribed in 48 CFR 1371.113, insert the following clause: Department of...

  13. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards for ship repair. As prescribed in 48 CFR 1371.113, insert the following clause: Department of...

  14. 42 CFR 137.271 - Why is there a separate subpart in these regulations for construction project agreements?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Why is there a separate subpart in these regulations for construction project agreements? 137.271 Section 137.271 Public Health PUBLIC HEALTH SERVICE... SELF-GOVERNANCE Construction Purpose and Scope § 137.271 Why is there a separate subpart in...

  15. 48 CFR 1352.271-75 - Delivery and shifting of the vessel.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... fairway of the facility. The contractor shall provide necessary tugs and pilot services to move the vessel....271-75 Delivery and shifting of the vessel. As prescribed in 48 CFR 1371.106, insert the...

  16. 48 CFR 1352.271-75 - Delivery and shifting of the vessel.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... fairway of the facility. The contractor shall provide necessary tugs and pilot services to move the vessel....271-75 Delivery and shifting of the vessel. As prescribed in 48 CFR 1371.106, insert the...

  17. 48 CFR 1352.271-75 - Delivery and shifting of the vessel.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... fairway of the facility. The contractor shall provide necessary tugs and pilot services to move the vessel....271-75 Delivery and shifting of the vessel. As prescribed in 48 CFR 1371.106, insert the...

  18. 48 CFR 1352.271-75 - Delivery and shifting of the vessel.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... fairway of the facility. The contractor shall provide necessary tugs and pilot services to move the vessel....271-75 Delivery and shifting of the vessel. As prescribed in 48 CFR 1371.106, insert the...

  19. 48 CFR 1352.271-85 - Documentation of requests for equitable adjustment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Clauses 1352.271-85 Documentation of requests for equitable adjustment. As prescribed in 48 CFR 1371.116..., the contractor shall submit a signed Certificate of Current Cost or Pricing Data. (d)...

  20. 48 CFR 1352.271-85 - Documentation of requests for equitable adjustment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Clauses 1352.271-85 Documentation of requests for equitable adjustment. As prescribed in 48 CFR 1371.116..., the contractor shall submit a signed Certificate of Current Cost or Pricing Data. (d)...

  1. 48 CFR 1352.271-85 - Documentation of requests for equitable adjustment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Clauses 1352.271-85 Documentation of requests for equitable adjustment. As prescribed in 48 CFR 1371.116..., the contractor shall submit a signed Certificate of Current Cost or Pricing Data. (d)...

  2. 48 CFR 1352.271-85 - Documentation of requests for equitable adjustment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Clauses 1352.271-85 Documentation of requests for equitable adjustment. As prescribed in 48 CFR 1371.116..., the contractor shall submit a signed Certificate of Current Cost or Pricing Data. (d)...

  3. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is responsible for administering, implementing, and coordinating the Agency's small business (including...

  4. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is responsible for administering, implementing, and coordinating the Agency's small business (including...

  5. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is responsible for administering, implementing, and coordinating the Agency's small business (including...

  6. Single CD271 marker isolates mesenchymal stem cells from human dental pulp.

    PubMed

    Alvarez, Ruth; Lee, Hye-Lim; Hong, Christine; Wang, Cun-Yu

    2015-12-01

    Mesenchymal stem cells (MSCs) are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow (BMSCs), adult MSCs are isolated from craniofacial tissues including dental pulp tissues (DPs) using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cells (DMSCs). In this study, we used different combinations of surface markers (CD51/CD140α, CD271, and STRO-1/CD146) to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells (DPCs) obtained from DP and compared their capacity to undergo multilineage differentiation. Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51(+)/CD140α(+), 10.6% were CD271(+), and 0.3% were STRO-1(+)/CD146(+). Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271(+) DMSCs demonstrated the greatest odontogenic potential. While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271(+) DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine. PMID:26674422

  7. LLNL's Response to 9/9/2009 Annual Inspection Report Comments B113 Issue #1 and B271 Issue #2

    SciTech Connect

    Schwartz, W W

    2009-11-19

    This is LLNL's responses to comments in the 9/9/2009 Annual Inspection Report concerning Underground Storage Tank (UST) 113-D1U2 (Issue No.1) at Building 113 and UST 271-D2U1 (Issue No.2) at Building 271. Also provided is the required Application for Underground Storage Tank Modification for USTs 113-D1U2 and 271-D2U1 and the specification sheet for the Phil-Tite spill bucket that is proposed to be installed in the 271-D2U1 sump.

  8. Light Variations of the Anomalous Central Star of Planetary Nebula Sh 2-71

    NASA Astrophysics Data System (ADS)

    Mikulášek, Z.; Skopal, A.; Zejda, M.; Pejcha, O.; Kohoutek, L.; Motl, D.; Vittone, A. A.; Errico, L.

    2007-03-01

    We present an analysis of light variations in UBV (RI)_{C} of the anomalous object in the center of planetary nebula Sh 2-71. We refined the linear ephemeris of the light maxima to JD_{max}=2449862.0+68.101(E-96), but also identified long-term, obviously non-periodic variations. The latter manifest themselves in large O-C shifts, a variable profile of light curves (hereafter LC) and changes in the mean brightness of the object. Our spectroscopic observations suggested the presence of a superdense nebula in the center of Sh 2-71.

  9. CD271 as a marker to identify mesenchymal stem cells from diverse sources before culture

    PubMed Central

    Álvarez-Viejo, María; Menéndez-Menéndez, Yolanda; Otero-Hernández, Jesús

    2015-01-01

    Mesenchymal stem cells, due to their characteristics are ideal candidates for cellular therapy. Currently, in culture these cells are defined by their adherence to plastic, specific surface antigen expression and multipotent differentiation potential. However, the in vivo identification of mesenchymal stem cells, before culture, is not so well established. Pre-culture identification markers would ensure higher purity than that obtained with selection based on adherence to plastic. Up until now, CD271 has been described as the most specific marker for the characterization and purification of human bone marrow mesenchymal stem cells. This marker has been shown to be specifically expressed by these cells. Thus, CD271 has been proposed as a versatile marker to selectively isolated and expand multipotent mesenchymal stem cells with both immunosuppressive and lymphohematopoietic engraftment-promoting properties. This review focuses on this marker, specifically on identification of mesenchymal stem cells from different tissues. Literature revision suggests that CD271 should not be defined as a universal marker to identify mesenchymal stem cells before culture from different sources. In the case of bone marrow or adipose tissue, CD271 could be considered a quite suitable marker; however this marker seems to be inadequate for the isolation of mesenchymal stem cells from other tissues such as umbilical cord blood or wharton’s jelly among others. PMID:25815130

  10. CD271 as a marker to identify mesenchymal stem cells from diverse sources before culture.

    PubMed

    Álvarez-Viejo, María; Menéndez-Menéndez, Yolanda; Otero-Hernández, Jesús

    2015-03-26

    Mesenchymal stem cells, due to their characteristics are ideal candidates for cellular therapy. Currently, in culture these cells are defined by their adherence to plastic, specific surface antigen expression and multipotent differentiation potential. However, the in vivo identification of mesenchymal stem cells, before culture, is not so well established. Pre-culture identification markers would ensure higher purity than that obtained with selection based on adherence to plastic. Up until now, CD271 has been described as the most specific marker for the characterization and purification of human bone marrow mesenchymal stem cells. This marker has been shown to be specifically expressed by these cells. Thus, CD271 has been proposed as a versatile marker to selectively isolated and expand multipotent mesenchymal stem cells with both immunosuppressive and lymphohematopoietic engraftment-promoting properties. This review focuses on this marker, specifically on identification of mesenchymal stem cells from different tissues. Literature revision suggests that CD271 should not be defined as a universal marker to identify mesenchymal stem cells before culture from different sources. In the case of bone marrow or adipose tissue, CD271 could be considered a quite suitable marker; however this marker seems to be inadequate for the isolation of mesenchymal stem cells from other tissues such as umbilical cord blood or wharton's jelly among others. PMID:25815130

  11. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Director, Office of Risk Assessment and Cost-Benefit... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  12. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Director, Office of Risk Assessment and Cost-Benefit... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  13. 47 CFR 80.271 - Technical requirements for portable survival craft radiotelephone transceivers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Technical requirements for portable survival... Authorization for Compulsory Ships § 80.271 Technical requirements for portable survival craft radiotelephone... procedure specified in the Radio Technical Commission for Marine Services (RTCM) Special Committee No....

  14. 47 CFR 80.271 - Technical requirements for portable survival craft radiotelephone transceivers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Technical requirements for portable survival... Authorization for Compulsory Ships § 80.271 Technical requirements for portable survival craft radiotelephone... procedure specified in the Radio Technical Commission for Marine Services (RTCM) Special Committee No....

  15. Hypoxia-Inducible Factor-1α and CD271 inversely correlate with melanoma invasiveness.

    PubMed

    Marconi, Alessandra; Borroni, Riccardo G; Truzzi, Francesca; Longo, Caterina; Pistoni, Federica; Pellacani, Giovanni; Pincelli, Carlo

    2015-05-01

    Melanoma is characterized, among other features, by microenvironmental factors and by an altered apoptotic machinery. Melanoma cell response to a hypoxic environment is transcriptionally regulated by the Hypoxia-Inducible Factor (HIF)-1α. p75 neurotrophin receptor (p75(NTR) ), also called CD271, mediates apoptosis in several cell systems. The purpose of this study was to analyze the expression of HIF-1α and CD271 in melanomas at different phases of progression, as evaluated by histology and reflectance confocal microscopy (RCM). By RCM, 41.67% tumors were characterized by the presence of a population of dendritic and pleomorphic cells (D+P), corresponding to in situ melanoma; 25% exhibited a predominantly round-cell (RN) proliferation with histologic features of superficial melanoma, and 33.33% showed the presence of cells aggregated in nests (DN), typical of invasive melanoma. HIF-1α was scarcely detected in D+P and in RN melanomas, while it was highly expressed in DN tumors. By contrast, CD271 positive cells were mostly detected in D+P population, and barely observed in the other subtypes. This work demonstrates that CD271 expression inversely correlates with hypoxia in melanoma, and that the two markers may be used in the future as diagnostic/prognostic tools for this neoplasm. PMID:25739328

  16. Age-related decrease in CD271(+) cells in human skin.

    PubMed

    Akamatsu, Hirohiko; Hasegawa, Seiji; Yamada, Takaaki; Mizutani, Hiroshi; Nakata, Satoru; Yagami, Akiko; Matsunaga, Kayoko

    2016-03-01

    According to recent studies, stem cells are found in various tissues in our bodies. It has been reported that stem cells can reside in the skin tissues, including the epidermis, dermis, hair follicles and subcutaneous tissues. Homeostasis of the skin is maintained because these stem cells collaborate with each other to form new cells. We previously identified the CD271(p75NTR)(+) cell as a stem cell that was present in the epidermis, dermis and subcutaneous tissue, and further investigated the role of stem cells in wound healing and their association with skin disease. In this study, we investigated the localization of CD271(+) cells in human skin (epidermis and dermis) and its age-related changes in stem cells using CD271(+) cells. The study revealed that the number of CD271(+) cells in the epidermis and dermis decreased with aging. It is possible that such an age-related decrease in stem cells causes impaired regenerative ability and is associated with various skin diseases. If the relationship between stem cells and skin aging and diseases can be elucidated by investigations such as this study, it may lead to the development of novel anti-aging technologies and medical treatments for skin diseases in the future. PMID:26300383

  17. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Clauses 1352.271-72 Additional Item Requirements (AIR)—growth work As prescribed in 48 CFR 1371.103... fees represent the Government's best estimate for growth that may be required throughout the contract..., Painting, Boilermaking, Pipe Fitting, Engineering (Production), Sheetmetal Work, Staging/Scaffolding,...

  18. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Clauses 1352.271-72 Additional Item Requirements (AIR)—growth work As prescribed in 48 CFR 1371.103... fees represent the Government's best estimate for growth that may be required throughout the contract..., Painting, Boilermaking, Pipe Fitting, Engineering (Production), Sheetmetal Work, Staging/Scaffolding,...

  19. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Insulated rail joints, bond wires, and track... STATE ACTION PLANS Maintenance, Inspection, and Testing Inspections and Tests § 234.271 Insulated rail joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections...

  20. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Insulated rail joints, bond wires, and track... STATE ACTION PLANS Maintenance, Inspection, and Testing Inspections and Tests § 234.271 Insulated rail joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections...

  1. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....271-70 Inspection and manner of doing work. As prescribed in 48 CFR 1371.101, insert the following... Marine Engineering Regulations and Material Specifications (46 CFR Subchapter F), U.S. Coast Guard Electrical Engineering Regulations (46 CFR Subchapter J), and U.S. Public Health Service “Handbook...

  2. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....271-70 Inspection and manner of doing work. As prescribed in 48 CFR 1371.101, insert the following... Marine Engineering Regulations and Material Specifications (46 CFR Subchapter F), U.S. Coast Guard Electrical Engineering Regulations (46 CFR Subchapter J), and U.S. Public Health Service “Handbook...

  3. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....271-70 Inspection and manner of doing work. As prescribed in 48 CFR 1371.101, insert the following... Marine Engineering Regulations and Material Specifications (46 CFR Subchapter F), U.S. Coast Guard Electrical Engineering Regulations (46 CFR Subchapter J), and U.S. Public Health Service “Handbook...

  4. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....271-70 Inspection and manner of doing work. As prescribed in 48 CFR 1371.101, insert the following... Marine Engineering Regulations and Material Specifications (46 CFR Subchapter F), U.S. Coast Guard Electrical Engineering Regulations (46 CFR Subchapter J), and U.S. Public Health Service “Handbook...

  5. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ....271-70 Inspection and manner of doing work. As prescribed in 48 CFR 1371.101, insert the following... Marine Engineering Regulations and Material Specifications (46 CFR Subchapter F), U.S. Coast Guard Electrical Engineering Regulations (46 CFR Subchapter J), and U.S. Public Health Service “Handbook...

  6. 34 CFR 271.1 - What is the State Educational Agency Desegregation Program?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false What is the State Educational Agency Desegregation... ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION STATE EDUCATIONAL AGENCY DESEGREGATION PROGRAM General § 271.1 What is the State Educational Agency Desegregation Program? This program provides...

  7. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Clauses 1352.271-72 Additional Item Requirements (AIR)—growth work As prescribed in 48 CFR 1371.103... requested by the Contracting Officer, material quotes shall be provided. (3) Subcontractor estimates, individually broken out and priced along with the actual subcontractor quotes. The requirement to...

  8. 26. BUILDING NO. 271I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. BUILDING NO. 271-I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER OF BUILDING SHOWING DOORS TO MIXING ROOM NO. 4. HAND CRANK VISIBLE AT RIGHT ROTATED SMALL POTS (CONTAINING LEAD AZIDE) IN MIXING ROOMS. - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  9. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c))....

  10. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c))....

  11. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c))....

  12. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c))....

  13. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c))....

  14. 42 CFR 137.271 - Why is there a separate subpart in these regulations for construction project agreements?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... regulations for construction project agreements? 137.271 Section 137.271 Public Health PUBLIC HEALTH SERVICE... regulations for construction project agreements? Construction projects are separately defined in Title V and are subject to a separate proposal and review process. Provisions of a construction project...

  15. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... for ship repair. As prescribed in 48 CFR 1371.113, insert the following clause: Department of Labor... under the contract, shall comply with the requirements of 29 CFR 1910.15. Nothing contained in this... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal...

  16. Epigenetic regulation of CD271, a potential cancer stem cell marker associated with chemoresistance and metastatic capacity

    PubMed Central

    LI, SULAN; YUE, DONGLI; CHEN, XINFENG; WANG, LIPING; LI, JIEYAO; PING, YU; GAO, QUN; WANG, DAN; ZHANG, TENGFEI; LI, FENG; YANG, LI; HUANG, LAN; ZHANG, YI

    2015-01-01

    Cancer stem cells (CSCs) are considered to be the cause of tumor initiation, metastasis and recurrence. Additionally, CSCs are responsible for the failure of chemotherapy and radiotherapy. The isolation and identification of CSCs is crucial for facilitating the monitoring, therapy or prevention of cancer. We aimed to identify esophageal squamous cell carcinoma (ESCC) stem-like cells, the epigenetic mechanism and identify novel biomarkers for targeting ESCC CSCs. Sixty-three paired ESCC tissues and adjacent non-cancerous tissues were included in this study. CD271, which was identified as the CSC marker for melanoma, was assessed using quantitative PCR (qPCR). Using flow cytometry, we isolated CD271+ cells comprising 7.5% of cancer cells from the KYSE70 cell line. Sphere formation and anchorage-independent growth were analyzed in CD271+ and CD271− cancer cells, respectively. qPCR was used to detect stem-related genes and CCK-8 was performed to analyze the sensitivity to chemotherapy in the two groups. Bisulfite genomic sequencing was used to analyze the methylation status. CD271 expression was significantly higher in ESCC tissues than in adjacent non-cancerous tissues. Compared with CD271− cancer cells, CD271+ cancer cells showed a higher ability of sphere and colony formation, a high level expression of stem-related gene, and resistance to chemotherapy. The expression of CD271 was induced by a demethylation agent. In conclusion, CD271+ ESCC cells possess stem-like properties. CD271 can potentially act as a prognostic marker for ESCC, whose expression is regulated epigenetically. PMID:25351876

  17. CD271 is a functional and targetable marker of tumor-initiating cells in head and neck squamous cell carcinoma

    PubMed Central

    Murillo-Sauca, Oihana; Chung, Man Ki; Shin, June Ho; Karamboulas, Christina; Kwok, Shirley; Jung, Young Ho; Oakley, Richard; Tysome, James R.; Farnebo, Lovisa O.; Kaplan, Michael J.; Sirjani, Davud; Divi, Vasu; Holsinger, F. Christopher; Tomeh, Chafeek; Nichols, Anthony; Le, Quynh T.; Colevas, A. A. Dimitrios; Kong, Christina S.; Uppaluri, Ravindra; Lewis, James S.; Ailles, Laurie E.; Sunwoo, John B.

    2014-01-01

    Tumor-initiating cells (TICs) in squamous cell carcinoma of the head and neck (SCCHN) are best characterized by their surface expression of CD44. Although there is great interest in identifying strategies to target this population, no marker of these cells has been found to be functionally active. Here, we examined the expression of the purported marker of normal human oral epithelial stem cells, CD271. We show that CD271 expression is restricted to a subset of the CD44+ cells. Using xenograft assays, we show that the CD44+CD271+ subpopulation contains the most tumorigenic cells. Loss of CD271 function results in a block in the G2-M phase of the cell cycle and a profound negative impact on the capacity of these cells to initiate tumor formation in vivo. Incubation with recombinant NGF results in enhanced phosphorylation of Erk, providing additional evidence that CD271 is functionally active. Finally, incubation of SCCHN cells with antibody to CD271 results in decreased Erk phosphorylation and decreased tumor formation in vivo. Thus, our data are the first to demonstrate that CD271 more specifically identifies the TIC subpopulation within the CD44+ compartment in SCCHN and that this receptor is a functionally active and targetable molecule. PMID:25149537

  18. The Isolated Interacting Galaxy Pair NGC 5426/27 (Arp 271)

    NASA Astrophysics Data System (ADS)

    Fuentes-Carrera, I.; Rosado, M.; Amram, P.; Dultzin-Hacyan, D.; Bernal, A.; Salo, H.; Laurikainen, E.; Cruz-González, I.; Le Coarer, E.

    2001-03-01

    The isolated interacting galaxy pair NGC 5426/27 (Arp 271) was observed using the scanning Fabry-Perot interferometer PUMA. The velocity field, various kinematical parameters and rotation curve for each galaxy were derived. We found a small bar-like structure in NGC 5426 and a severely distorted velocity field for NGC 5427. A range of possible masses was computed for each galaxy.

  19. Modifications to the Finnigan MAT 271 mass spectrometer in the Inorganic Gas Analysis Lab

    SciTech Connect

    Reber, S.D.; Cordes, G.T.

    1995-08-01

    This document presents an overview of the modifications that were done to the Finnigan MAT 271 mass spectrometer used in the Dept. 1823 Inorganic Gas Analysis Lab. Among the alterations to the spectrometer were addition of a new computer, interfaces to the power supply, addition of a multimeter and introduction of a Graphical User Interface software system to run the instrument. The impact of these improvements is also discussed. The appendix details a generic procedure for operating the instrument.

  20. 27. BUILDING NO. 271I, INTERIOR OF LEAD AZIDE MIXING ROOM. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. BUILDING NO. 271-I, INTERIOR OF LEAD AZIDE MIXING ROOM. HERE, TWO POUNDS (MAXIMUM) OF LEAD AZIDE WAS MIXED IN SEVEN SMALL POTS EACH SEPARATED FROM THE OTHERS BY THE STEEL WALLS PICTURED HERE. THE POTS ARE ALL FIXED TO A COMMON SHAFT WHICH IS ROTATED BY A HAND CRANK OUTSIDE OF ROOM (SEE HAER NJ-36A-26). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  1. Examining the Feasibility of Clinical Grade CD271+ Enrichment of Mesenchymal Stromal Cells for Bone Regeneration

    PubMed Central

    Cuthbert, Richard J.; Giannoudis, Peter V.; Wang, Xiao N.; Nicholson, Lindsay; Pawson, David; Lubenko, Anatole; Tan, Hiang B.; Dickinson, Anne; McGonagle, Dennis; Jones, Elena

    2015-01-01

    Introduction Current clinical trials utilize mesenchymal stromal cells (MSCs) expanded in culture, however these interventions carry considerable costs and concerns pertaining to culture-induced losses of potency. This study assessed the feasibility of new clinical-grade technology to obtain uncultured MSC isolates from three human intra-osseous tissue sources based on immunomagnetic selection for CD271-positive cells. Materials and Methods MSCs were isolated from bone marrow (BM) aspirates or surgical waste materials; enzymatically digested femoral heads (FHs) and reamer irrigator aspirator (RIA) waste fluids. Flow cytometry for the CD45−/lowCD73+CD271+ phenotype was used to evaluate uncultured MSCs before and after selection, and to measure MSC enrichment in parallel to colony forming-unit fibroblast assay. Trilineage differentiation assays and quantitative polymerase chain-reaction for key transcripts involved in bone regeneration was used to assess the functional utility of isolated cells for bone repair. Results Uncultured CD45−/lowCD271+ MSCs uniformly expressed CD73, CD90 and CD105 but showed variable expression of MSCA-1 and SUSD2 (BM>RIA>FH). MSCs were enriched over 150-fold from BM aspirates and RIA fluids, whereas the highest MSC purities were obtained from FH digests. Enriched fractions expressed increased levels of BMP-2, COL1A2, VEGFC, SPARC and CXCL12 transcripts (BM>RIA>FH), with the highest up-regulation detected for CXCL12 in BM (>1300-fold). Following culture expansion, CD271-selected MSCS were tri-potential and phenotypically identical to plastic adherence-selected MSCs. Discussion A CD271-based GMP-compliant immunomagnetic selection resulted in a substantial increase in MSC purity and elevated expression of transcripts involved in bone formation, vascularisation and chemo-attraction. Although this technology, particularly from RIA fluids, can be immediately applied by orthopaedic surgeons as autologous therapy, further improvements in MSC

  2. Acceptance test procedure bldg. 271-U remote monitoring of project W-059 B-Plant canyon exhaust system

    SciTech Connect

    MCDANIEL, K.S.

    1999-09-01

    The test procedure provides for verifying indications and alarms The test procedure provides for verifying indications and alarms associated with the B Plant Canyon Ventilation System as they are being displayed on a remote monitoring workstation located in building 271-U. The system application software was installed by PLCS Plus under contract from B&W Hanford Company. The application software was installed on an existing operator workstation in building 271U which is owned and operated by Bechtel Hanford Inc.

  3. Active-site mutagenesis of tetanus neurotoxin implicates TYR-375 and GLU-271 in metalloproteolytic activity.

    PubMed

    Rossetto, O; Caccin, P; Rigoni, M; Tonello, F; Bortoletto, N; Stevens, R C; Montecucco, C

    2001-08-01

    Tetanus neurotoxin (TeNT) blocks neurotransmitter release by cleaving VAMP/synaptobrevin, a membrane associated protein involved in synaptic vesicle fusion. Such activity is exerted by the N-terminal 50kDa domain of TeNT which is a zinc-dependent endopeptidase (TeNT-L-chain). Based on the three-dimensional structure of botulinum neurotoxin serotype A (BoNT/A) and serotype B (BoNT/B), two proteins closely related to TeNT, and on X-ray scattering studies of TeNT, we have designed mutations at two active site residues to probe their involvement in activity. The active site of metalloproteases is composed of a primary sphere of residues co-ordinating the zinc atom, and a secondary sphere of residues that determines proteolytic specificity and activity. Glu-261 and Glu-267 directly co-ordinates the zinc atom in BoNT/A and BoNT/B respectively and the corresponding residue of TeNT was replaced by Asp or by the non conservative residue Ala. Tyr-365 is 4.3A away from zinc in BoNT/A, and the corresponding residue of TeNT was replaced by Phe or by Ala. The purified mutants had CD, fluorescence and UV spectra closely similar to those of the wild-type molecule. The proteolytic activity of TeNT-Asp-271 (E271D) is similar to that of the native molecule, whereas that of TeNT-Phe-375 (Y375F) is lower than the control. Interestingly, the two Ala mutants are completely devoid of enzymatic activity. These results demonstrate that both Glu-271 and Tyr-375 are essential for the proteolytic activity of TeNT. PMID:11306125

  4. CD271+ Mesenchymal Stem Cells as a Possible Infectious Niche for Leishmania infantum.

    PubMed

    Lopes, Carolina S; Daifalla, Nada; Das, Bikul; Dias da Silva, Valdo; Campos-Neto, Antonio

    2016-01-01

    Visceral leishmaniasis (VL) is a serious and fatal disease. Therapeutic drugs are toxic and non-sterilizing. The etiological agents Leishmania infantum and Leishmania donovani cause active and asymptomatic diseases. Effective drugs to treat VL exist but unfortunately, post-treatment relapses are common. Little is known why drugs are non-sterilizing or how these intracellular pathogens can escape treatment. Here, using a murine model of VL we found that CD271+/Sca1+ bone marrow mesenchymal stem cells (BM-MSCs) are readily infected in vitro and in vivo by L. infantum. Because BM-MSCs express potent drug efflux pumps, e.g., ABCG2 it is possible that this unique intracellular infectious niche could allow L. infantum to escape anti-parasite drugs. PMID:27622907

  5. {alpha} decay chains in {sup 271-294}115 superheavy nuclei

    SciTech Connect

    Santhosh, K. P.; Priyanka, B.; Joseph, Jayesh George; Sahadevan, Sabina

    2011-08-15

    {alpha} decay of {sup 271-294}115 superheavy nuclei is studied using the Coulomb and proximity potential model for deformed nuclei (CPPMDN). The predicted {alpha} half-lives of {sup 287}115 and {sup 288}115 nuclei and their decay products are in good agreement with experimental values. Comparison of {alpha} and spontaneous fission half-lives predicts four-{alpha} chains and three-{alpha} chains, respectively, from {sup 287}115 and {sup 288}115 nuclei and are in agreement with experimental observation. Our study predicts two-{alpha} chains from {sup 273,274,289}115, three-{alpha} chains from {sup 275}115, and four-{alpha} chains consistently from {sup 284,285,286}115 nuclei. These observations will be useful for further experimental investigation in this region.

  6. Site-directed mutagenesis of Arg60 and Cys271 in ornithine transcarbamylase from rat liver.

    PubMed

    McDowall, S; van Heeswijck, R; Hoogenraad, N

    1990-10-01

    We have investigated the putative carbamylphosphate- and ornithine-binding domains in ornithine transcarbamylase from rat liver using site-directed mutagenesis. Arg60, present in the phosphate-binding motif X-Ser-X-Arg-X and therefore implicated in the binding of the phosphate moiety of carbamylphosphate has been replaced with a leucine. This results in a dramatic reduction of catalytic activity, although the enzyme is synthesized in cells stably transfected with the mutant clone and imported, correctly processed and assembled into a homotrimer in mitochondria. The sole cysteine residue (Cys271) has been implicated in ornithine binding by the chemical modification studies of Marshall and Cohen in 1972 and 1980 (J. Biol. Chem., 247, 1654-1668, 1669-1682; 255, 7291-7295, 7296-7300). Replacement of this residue with serine did not eliminate enzyme activity but affected the Michaelis constant for ornithine (Kb), increasing it 5-fold from 0.71 to 3.7 mM and reduced the kcat at pH 8.5 by 20-fold. These changes represent a loss in apparent binding energy for the enzyme--ornithine complex of 2.9 kcal/mol, suggesting that Cys271 is normally involved in hydrogen bonding to the substrate, ornithine. The cysteine to serine substitution also caused the dissociation constant (Kii) for the competitive inhibitor, L-norvaline to be increased 10-fold, from 12 to 120 microM. The small loss in binding energy and relatively high residual catalytic activity of the mutant strongly suggests that a number of other residues are involved in the binding of ornithine.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2290837

  7. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and Renewable Energy 2923.271 Purchase and use of environmentally sound and energy efficient products and...-efficient products, alternate fuel vehicles, and products using renewable energy. (1) To the...

  8. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and Renewable Energy 2923.271 Purchase and use of environmentally sound and energy efficient products and...-efficient products, alternate fuel vehicles, and products using renewable energy. (1) To the...

  9. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and Renewable Energy 2923.271 Purchase and use of environmentally sound and energy efficient products and...-efficient products, alternate fuel vehicles, and products using renewable energy. (1) To the...

  10. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and Renewable Energy 2923.271 Purchase and use of environmentally sound and energy efficient products and...-efficient products, alternate fuel vehicles, and products using renewable energy. (1) To the...

  11. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and Renewable Energy 2923.271 Purchase and use of environmentally sound and energy efficient products and...-efficient products, alternate fuel vehicles, and products using renewable energy. (1) To the...

  12. Mesenchymal stem cells expressing GD2 and CD271 correlate with breast cancer-initiating cells in bone marrow

    PubMed Central

    Cohen, Evan N; Gao, Hui; Mego, Michal; Lee, Bang-Ning; Lodhi, Ashutosh; Cristofanilli, Massimo; Lucci, Anthony

    2011-01-01

    Purpose The bone marrow microenvironment is considered a critical component in the dissemination and fate of cancer cells in the metastatic process. We explored the possible correlation between bone marrow mesenchymal stem cells (BM-MSC) and disseminated breast cancer-initiating cells (BCIC) in primary breast cancer patients. Results The percentages of BCIC (Aldefluor+CD326+CD44+CD24−) correlated with the percentages of BM-MSC, either CD45−GD2+CD200+CD271+ (Kedall's τ = 0.684, p = 0.004) or CD45−GD2+CD271+ in the bone marrow (Kedall's τ = 0.464, p = 0.042). Experimental Design Bone marrow mononuclear cells (BM-MNC) were collected at the time of primary surgery in 12 breast cancer patients. BM-MNC was immunophenotyped and BCIC was defined as epithelial cells (CD326+CD45−) with a “stem-like” phenotype (CD44+CD24low/−, ALDH activity). BM-MSC was defined as CD34−CD45− cells that co-expressed GD2, CD271 and/or CD200 within CD326-depleted BM-MNC. Conclusions There was a positive correlation between mesenchymal stem cells expressing GD2 and CD271 and breast cancer-initiating cells in BM of patients with primary breast cancer. PMID:21358274

  13. CD271 antigen defines a subset of multipotent stromal cells with immunosuppressive and lymphohematopoietic engraftment-promoting properties

    PubMed Central

    Kuçi, Selim; Kuçi, Zyrafete; Kreyenberg, Hermann; Deak, Erika; Pütsch, Kathrin; Huenecke, Sabine; Amara, Chandrasekhar; Koller, Stefanie; Rettinger, Eva; Grez, Manuel; Koehl, Ulrike; Latifi-Pupovci, Hatixhe; Henschler, Reinhard; Tonn, Torsten; von Laer, Dorothee; Klingebiel, Thomas; Bader, Peter

    2010-01-01

    Background In vitro proliferative and differentiation potential of mesenchymal stromal cells generated from CD271+ bone marrow mononuclear cells (CD271-mesenchymal stromal cells) has been demonstrated in several earlier and recent reports. In the present study we focused, in addition to proliferative and differentiation potential, on in vitro and in vivo immunosuppressive and lymphohematopoietic engraftment-promoting potential of these mesenchymal stromal cells compared to bone marrow-derived mesenchymal stromal cells generated by plastic adherence (plastic adherence-mesenchymal stromal cells). Design and Methods We set up a series of experimental protocols in order to determine the phenotype of CD271-mesenchymal stromal cells, and their clonogenic, proliferative, differentiation and immunosuppressive potential. The potential of CD271-mesenchymal stromal cells to improve the engraftment of CD133+ hematopoietic stem cells at co-transplantation was evaluated in immunodeficient NOD/SCID-IL2Rγnull mice. Results In vitro studies demonstrated that CD271-mesenchymal stromal cells differentiate along adipogenic, osteogenic and chondrogenic lineages (trilineage potential), produce significantly higher levels of cytokines than plastic adherence-mesenchymal stromal cells, and significantly inhibit the proliferation of allogeneic T-lymphocytes in mixed lymphocyte reaction assays. Elevated levels of prostaglandin E2, but not nitric monoxide, mediated the majority of this immunosuppressive effect. In vivo studies showed that CD271-mesenchymal stromal cells promoted significantly greater lymphoid engraftment than did plastic adherence-mesenchymal stromal cells when co-transplanted with CD133+ hematopoietic stem cells at a ratio of 8:1 in immunodeficient NOD/SCID-IL2Rγnull mice. They induced a 10.4-fold increase in the number of T cells, a 2.5-fold increase in the number of NK cells, and a 3.6-fold increase in the number of B cells, indicating a major qualitative difference

  14. Flavodiiron oxygen reductase from Entamoeba histolytica: modulation of substrate preference by tyrosine 271 and lysine 53.

    PubMed

    Gonçalves, Vera L; Vicente, João B; Pinto, Liliana; Romão, Célia V; Frazão, Carlos; Sarti, Paolo; Giuffrè, Alessandro; Teixeira, Miguel

    2014-10-10

    Flavodiiron proteins (FDPs) are a family of enzymes endowed with bona fide oxygen- and/or nitric-oxide reductase activity, although their substrate specificity determinants remain elusive. After a comprehensive comparison of available three-dimensional structures, particularly of FDPs with a clear preference toward either O2 or NO, two main differences were identified near the diiron active site, which led to the construction of site-directed mutants of Tyr(271) and Lys(53) in the oxygen reducing Entamoeba histolytica EhFdp1. The biochemical and biophysical properties of these mutants were studied by UV-visible and electron paramagnetic resonance (EPR) spectroscopies coupled to potentiometry. Their reactivity with O2 and NO was analyzed by stopped-flow absorption spectroscopy and amperometric methods. These mutations, whereas keeping the overall properties of the redox cofactors, resulted in increased NO reductase activity and faster inactivation of the enzyme in the reaction with O2, pointing to a role of the mutated residues in substrate selectivity. PMID:25151360

  15. Ethanol induces upregulation of the nerve growth factor receptor CD271 in human melanoma cells via nuclear factor-κB activation

    PubMed Central

    RAPPA, GERMANA; ANZANELLO, FABIO; LORICO, AURELIO

    2015-01-01

    Alcohol consumption is one of the most important, and potentially avoidable, risk factors of human cancer, accounting for 3.6% of all types of cancer worldwide. In a recent meta-analysis, a 20% increased risk of melanoma was linked with regular alcohol consumption. In the present study, the effect of ethanol exposure on the expression of the nerve growth factor receptor, CD271, in human FEMX-I melanoma cells was investigated. Consistent with the derivation of melanocytes from the neural crest, the majority of melanomas express CD271, a protein that is crucial for maintaining the melanoma stem cell properties, including the capacity of self-renewal and resistance to chemotherapy and radiotherapy. Analysis of CD271-sorted subpopulations and clones of FEMX-I cells indicated no hierarchical organization of CD271+ and CD271− cells. In addition, CD271 expression was lost upon growth of FEMX-I melanoma cells in cancer stem cell-like conditions, while it was greatly increased upon CD133 knockdown or exposure to ethanol. After 24-h exposure to 100, 200 and 400 mM ethanol, the percentage of CD271+ cells increased from 14% in control cells to 24, 35 and 88%, respectively. An increase in the percentage of CD271+ cells was already evident 8 h after ethanol exposure and reached a maximum at 48 h. Ethanol-induced upregulation of CD271 was mediated by nuclear factor-κB (NF-κB). In fact, exposure of FEMX-I cells to 100–400 mM ethanol for 24 h resulted in a concentration- and time-dependent increase in NF-κB activity, up to 900% that of control cells. NF-κB activation was due to a decrease in p50 homodimers, which occupy the NF-κB binding site, blocking transactivation. No effects of ethanol on 9 additional signaling pathways of FEMX-I cells were observed. In the presence of CD271 blocking antibodies, NF-κB activation was not prevented, indicating that ethanol did not target CD271 directly. These data demonstrate that ethanol induces expression of CD271 in FEMX-I cells via

  16. Defective Ti2Nb10O27.1: an advanced anode material for lithium-ion batteries

    NASA Astrophysics Data System (ADS)

    Lin, Chunfu; Yu, Shu; Zhao, Hua; Wu, Shunqing; Wang, Guizhen; Yu, Lei; Li, Yanfang; Zhu, Zi-Zhong; Li, Jianbao; Lin, Shiwei

    2015-12-01

    To explore anode materials with large capacities and high rate performances for the lithium-ion batteries of electric vehicles, defective Ti2Nb10O27.1 has been prepared through a facile solid-state reaction in argon. X-ray diffractions combined with Rietveld refinements indicate that Ti2Nb10O27.1 has the same crystal structure with stoichiometric Ti2Nb10O29 (Wadsley-Roth shear structure with A2/m space group) but larger lattice parameters and 6.6% O2- vacancies (vs. all O2- ions). The electronic conductivity and Li+ion diffusion coefficient of Ti2Nb10O27.1 are at least six orders of magnitude and ~2.5 times larger than those of Ti2Nb10O29, respectively. First-principles calculations reveal that the significantly enhanced electronic conductivity is attributed to the formation of impurity bands in Ti2Nb10O29-x and its conductor characteristic. As a result of the improvements in the electronic and ionic conductivities, Ti2Nb10O27.1 exhibits not only a large initial discharge capacity of 329 mAh g-1 and charge capacity of 286 mAh g-1 at 0.1 C but also an outstanding rate performance and cyclability. At 5 C, its charge capacity remains 180 mAh g-1 with large capacity retention of 91.0% after 100 cycles, whereas those of Ti2Nb10O29 are only 90 mAh g-1 and 74.7%.

  17. Defective Ti2Nb10O27.1: an advanced anode material for lithium-ion batteries.

    PubMed

    Lin, Chunfu; Yu, Shu; Zhao, Hua; Wu, Shunqing; Wang, Guizhen; Yu, Lei; Li, Yanfang; Zhu, Zi-Zhong; Li, Jianbao; Lin, Shiwei

    2015-01-01

    To explore anode materials with large capacities and high rate performances for the lithium-ion batteries of electric vehicles, defective Ti2Nb10O27.1 has been prepared through a facile solid-state reaction in argon. X-ray diffractions combined with Rietveld refinements indicate that Ti2Nb10O27.1 has the same crystal structure with stoichiometric Ti2Nb10O29 (Wadsley-Roth shear structure with A2/m space group) but larger lattice parameters and 6.6% O(2-) vacancies (vs. all O(2-) ions). The electronic conductivity and Li(+)ion diffusion coefficient of Ti2Nb10O27.1 are at least six orders of magnitude and ~2.5 times larger than those of Ti2Nb10O29, respectively. First-principles calculations reveal that the significantly enhanced electronic conductivity is attributed to the formation of impurity bands in Ti2Nb10O29-x and its conductor characteristic. As a result of the improvements in the electronic and ionic conductivities, Ti2Nb10O27.1 exhibits not only a large initial discharge capacity of 329 mAh g(-1) and charge capacity of 286 mAh g(-1) at 0.1 C but also an outstanding rate performance and cyclability. At 5 C, its charge capacity remains 180 mAh g(-1) with large capacity retention of 91.0% after 100 cycles, whereas those of Ti2Nb10O29 are only 90 mAh g(-1) and 74.7%. PMID:26632883

  18. Defective Ti2Nb10O27.1: an advanced anode material for lithium-ion batteries

    PubMed Central

    Lin, Chunfu; Yu, Shu; Zhao, Hua; Wu, Shunqing; Wang, Guizhen; Yu, Lei; Li, Yanfang; Zhu, Zi-Zhong; Li, Jianbao; Lin, Shiwei

    2015-01-01

    To explore anode materials with large capacities and high rate performances for the lithium-ion batteries of electric vehicles, defective Ti2Nb10O27.1 has been prepared through a facile solid-state reaction in argon. X-ray diffractions combined with Rietveld refinements indicate that Ti2Nb10O27.1 has the same crystal structure with stoichiometric Ti2Nb10O29 (Wadsley-Roth shear structure with A2/m space group) but larger lattice parameters and 6.6% O2– vacancies (vs. all O2– ions). The electronic conductivity and Li+ion diffusion coefficient of Ti2Nb10O27.1 are at least six orders of magnitude and ~2.5 times larger than those of Ti2Nb10O29, respectively. First-principles calculations reveal that the significantly enhanced electronic conductivity is attributed to the formation of impurity bands in Ti2Nb10O29–x and its conductor characteristic. As a result of the improvements in the electronic and ionic conductivities, Ti2Nb10O27.1 exhibits not only a large initial discharge capacity of 329 mAh g–1 and charge capacity of 286 mAh g–1 at 0.1 C but also an outstanding rate performance and cyclability. At 5 C, its charge capacity remains 180 mAh g–1 with large capacity retention of 91.0% after 100 cycles, whereas those of Ti2Nb10O29 are only 90 mAh g–1 and 74.7%. PMID:26632883

  19. Corrective Action Plan for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems, Nevada Test Site, Nevada

    SciTech Connect

    R. B. Jackson

    2003-05-01

    The Areas 25, 26 and 27 Septic Systems are in the Federal Facility Agreement and Consent Order (FFACO) of 1996 as Corrective Action Unit (CAU) 271. This Corrective Action Plan (CAP) provides selected corrective action alternatives and proposes the closure methodology for CAU 271. CAU 271 is located on the Nevada Test Site (NTS) approximately 105 kilometers (65 miles) northwest of Las Vegas, Nevada, and consists of the following 15 Corrective Action Sites (CAS): CAS 25-04-1, Septic System; CAS 25-04-03, Septic System; CAS25-04-04, Septic System; CAS 25-04-08, Septic System; CAS 25-04-09, Septic System; CAS 25-04-10, Septic System; CAS 25-04-11, Septic System; CAS 26-03-01, Contaminated Water Reservoir; CAS 26-04-1, Septic System; CAS 26-04-02, Septic System; CAS 26-05-01, Radioactive Leachfield; CAS-26-05-03, Septic System; CAS 26-05-04, Septic System; CAS 26-05-05, Septic System; and CAS 27-05-02, Leachfield.

  20. Increased CD271 expression by the NF-kB pathway promotes melanoma cell survival and drives acquired resistance to BRAF inhibitor vemurafenib

    PubMed Central

    Lehraiki, Abdelali; Cerezo, Michael; Rouaud, Florian; Abbe, Patricia; Allegra, Marilyne; Kluza, Jerome; Marchetti, Philippe; Imbert, Veronique; Cheli, Yann; Bertolotto, Corine; Ballotti, Robert; Rocchi, Stéphane

    2015-01-01

    Specific BRAFV600E inhibitors (BRAFi) are highly effective in the treatment of melanoma. However, acquired drug resistances invariably develop after the initial response. Therefore, the identification of new mechanisms of acquired resistance gives important clues towards the development of therapies that could elicit long lasting responses. Here we report that CD271 confers resistance to BRAFi in melanoma cells. The expression of CD271 is increased by BRAFi through a stimulation of tumor necrosis factor-alpha (TNFα) secretion that leads to NF-κB signaling pathway activation. CD271 is upregulated in a subset of BRAFi-resistant melanoma cells. The inhibition of TNFα/NF-κB pathway and CD271 silencing restore the BRAFi sensitivity of resistant melanoma cells. Finally, increase of CD271 expression is validated in BRAFi-resistant xenografts tumors and also in tumors from the patients who relapsed under BRAFi. In summary, these results reveal a novel TNFα/NF-κB/CD271 axis whose activation contributes to the acquisition of resistance to BRAFi and therefore may represent a novel therapeutic target to improve the efficacy of therapy in melanoma.

  1. Antibody Therapy Targeting CD47 and CD271 Effectively Suppresses Melanoma Metastasis in Patient-Derived Xenografts.

    PubMed

    Ngo, Michael; Han, Arum; Lakatos, Anita; Sahoo, Debashis; Hachey, Stephanie J; Weiskopf, Kipp; Beck, Andrew H; Weissman, Irving L; Boiko, Alexander D

    2016-08-01

    The high rate of metastasis and recurrence among melanoma patients indicates the existence of cells within melanoma that have the ability to both initiate metastatic programs and bypass immune recognition. Here, we identify CD47 as a regulator of melanoma tumor metastasis and immune evasion. Protein and gene expression analysis of clinical melanoma samples reveals that CD47, an anti-phagocytic signal, correlates with melanoma metastasis. Antibody-mediated blockade of CD47 coupled with targeting of CD271(+) melanoma cells strongly inhibits tumor metastasis in patient-derived xenografts. This therapeutic effect is mediated by drastic changes in the tumor and metastatic site immune microenvironments, both of whichwhich exhibit greatly increased density of differentiated macrophages and significantly fewer inflammatory monocytes, pro-metastatic macrophages (CCR2(+)/VEGFR1(+)), and neutrophils, all of which are associated with disease progression. Thus, antibody therapy that activates the innate immune response in combination with selective targeting of CD271(+) melanoma cells represents a powerful therapeutic approach against metastatic melanoma. PMID:27477289

  2. Rates of upgrade to malignancy for 271 cases of flat epithelial atypia (FEA) diagnosed by breast core biopsy.

    PubMed

    Peres, Alexandre; Barranger, Emmanuel; Becette, Véronique; Boudinet, Alain; Guinebretiere, Jean-Marc; Cherel, Pascal

    2012-06-01

    Flat epithelial atypia (FEA) is a borderline lesion that might represent an early stage in the development of certain low-grade carcinomas in situ and invasive cancers. There are no guidelines on its management. Our objectives were to determine the upgrade to malignancy rate and identify a subpopulation of patients that might undergo just mammographic surveillance. We retrospectively reviewed the data for 271 FEA cases among 5,555 breast core biopsies obtained over a 7-year period (January 2003-2010). We collated clinical data (age, history of cancer, menopausal status), radiological data (lesion type, size, Bi-Rads category), technical data (number of biopsies, needle gauge, excision quality) and histological data and sought correlations between these factors and upgrade rate. The 271 FEA comprised 128 cases of pure FEA, 135 cases of FEA + atypical ductal hyperplasia, and 8 cases of FEA + atypical lobular hyperplasia. Overall, 184 patients underwent surgery and 46 mammographic surveillance. Surgery detected 34 cases of malignancy (23 CIS, 7 invasive cancers, and 4 mixed cases) giving a 15% upgrade rate. Quality of excision was the only factor associated with under-diagnosis. The presence of FEA at biopsy warrants surgery. PMID:22042365

  3. Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1

    PubMed Central

    Liang, Yan-Ling; Zhang, Zheng; Wu, Min; Wu, Yuan

    2014-01-01

    From different natural reserves in the subtropical region of China, a total of 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as the sole carbon source. Of the 245 strains, 22 showed hydrolyzing zones on agar plates containing carboxymethyl cellulose after Congo-red staining. Molecular identification showed that the 22 strains belonged to 10 different genera, with the Burkholderia genus exhibiting the highest strain diversity and accounting for 36.36% of all the 22 strains. Three isolates among the 22 strains showed higher carboxymethyl cellulase (CMCase) activity, and isolate ME27-1 exhibited the highest CMCase activity in liquid culture. The strain ME27-1 was identified as Paenibacillus terrae on the basis of 16S rRNA gene sequence analysis as well as physiological and biochemical properties. The optimum pH and temperature for CMCase activity produced by the strain ME27-1 were 5.5 and 50°C, respectively, and the enzyme was stable at a wide pH range of 5.0–9.5. A 12-fold improvement in the CMCase activity (2.08 U/mL) of ME27-1 was obtained under optimal conditions for CMCase production. Thus, this study provided further information about the diversity of cellulose-degrading bacteria in the subtropical region of China and found P. terrae ME27-1 to be highly cellulolytic. PMID:25050355

  4. Proposed ionic bond between Arg300 and Glu270 and Glu271 are not involved in inactivation of a mutant firefly luciferase (LRR).

    PubMed

    Sobhani-Damavandifar, Zahra; Hosseinkhani, Saman; Sajedi, Reza H

    2016-05-01

    The weakness of firefly luciferase is its rapid inactivation. Many studies have been done to develop thermostable luciferases. One of these modifications was LRR mutant in which the Leu300 was substituted with Arg in the E(354)RR(356)Lampyris turkestanicus luciferase as template. LRR was more thermostable than the wild type but with only 0.02% activity. In this study, site-directed mutagenesis was used to change the proposed ionic bond between the Arg and two neighboring residues (Glu270 and Glu271), to understand if the induced interactions were responsible for inactivation in LRR. Our results showed that substitution of Glu270 and 271 with Ala removed the interactions but the activity of enzyme did not return. The E270A mutant was more active than LRR but the E271A and E270A/E271A mutants were inactive. Fluorescence and CD measurements showed that these mutations were accompanied by conformational changes. Extrinsic fluorescence measurement and obtained quenching data by KI and acrylamide also confirmed that the mutants were less compact than the LRR enzyme. In conclusion, in LRR, the interactions between Arg300 and Glu270 and Glu271 were not responsible for the enzyme inactivation and it is proposed that the enzyme inactivation is due to conformational changes of LRR mutant of firefly luciferase. PMID:26992788

  5. HL271, a novel chemical compound derived from metformin, differs from metformin in its effects on the circadian clock and metabolism.

    PubMed

    Row, Hansang; Jeong, Jaekap; Cho, Sehyung; Kim, Sungwuk; Kim, Kyungjin

    2016-01-15

    Metformin is a treatment of choice for patients with type 2 diabetes. Its action involves the phosphorylation of 5'-adenosine monophosphate activated protein kinase (AMPK), leading to inhibition of liver gluconeogenesis. The effects of a novel chemical compound derived from metformin, HL271, on molecular and physiological actions involving AMPK and rhythmically-expressed circadian clock genes were investigated. HL271 potently activated AMPK in a dose-dependent manner, and produced shortening of the circadian period and enhanced degradation of the clock genes PER2 and CRY1. Although the molecular effects of HL271 resembled those of metformin, it produced different physiological effects in mice with diet-induced obesity. HL271 did not elicit glucose-lowering or insulin-sensitizing effects, possibly because of altered regulation of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase 1. This indicated that, although HL271 acted on circadian clock machinery through a similar molecular mechanism to metformin, it differed in its systemic effect on glucose and lipid metabolite regulations. PMID:26707879

  6. Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV.

    PubMed

    Fukuchi, Mikoto; Wanotayan, Rujira; Liu, Sicheng; Imamichi, Shoji; Sharma, Mukesh Kumar; Matsumoto, Yoshihisa

    2015-06-12

    XRCC4 and DNA Ligase IV (LIG4) cooperate to join two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). However, it is not fully understood how these proteins are localized to the nucleus. Here we created XRCC4(K271R) mutant, as Lys271 lies within the putative nuclear localization signal (NLS), and XRCC4(K210R) mutant, as Lys210 was reported to undergo SUMOylation, implicated in the nuclear localization of XRCC4. Wild-type and mutated XRCC4 with EGFP tag were introduced into HeLa cell, in which endogenous XRCC4 had been knocked down using siRNA directed to 3'-untranslated region, and tested for the nuclear localization function by fluorescence microscopy. XRCC4(K271R) was defective in the nuclear localization of itself and LIG4, whereas XRCC4(K210R) was competent for the nuclear localization with LIG4. To examine DSB repair function, wild-type and mutated XRCC4 were introduced into XRCC4-deficient M10. M10-XRCC4(K271R), but not M10-XRCC4(K210R), showed significantly reduced surviving fraction after 2 Gy γ-ray irradiation as compared to M10-XRCC4(WT). The number of γ-H2AX foci remaining 2 h after 2 Gy γ-ray irradiation was significantly greater in M10-XRCC4(K271R) than in M10-XRCC4(WT), whereas it was only marginally increased in M10-XRCC4(K210R) as compared to M10-XRCC4(WT). The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4. PMID:25934149

  7. Clonal analysis of multipotent stromal cells derived from CD271+ bone marrow mononuclear cells: functional heterogeneity and different mechanisms of allosuppression

    PubMed Central

    Kuçi, Zyrafete; Seiberth, Julia; Latifi-Pupovci, Hatixhe; Wehner, Sibylle; Stein, Stefan; Grez, Manuel; Bönig, Halvard; Köhl, Ulrike; Klingebiel, Thomas; Bader, Peter; Kuçi, Selim

    2013-01-01

    Previous reports demonstrated a relationship between proliferation potential and trilineage differentiation in mesenchymal stromal cell-derived clones generated using plastic adherence (PA-MSCs). However, there are no reports presenting a clonal analysis of the proliferative potential, differentiation potential and allosuppressive effects of human mesenchymal stromal cell subsets. In this study, we performed a clonal analysis of mesenchymal stromal cells generated from human CD271+ bone marrow mononuclear cells (CD271-MSCs). After transfection with the gene encoding green fluorescent protein, the cells were single-cell sorted and cultured for 2–4 weeks. A population doubling analysis demonstrated that 25% of CD271-MSC clones are fast-proliferating clones compared to only 10% of PA-MSC clones. Evaluation of the allosuppressive potential demonstrated that 81.8% of CD271-MSC clones were highly allosuppressive compared to only 58% of PA-MSC clones. However, no consistent correlation was observed between allosuppression and proliferative potential. Prostaglandin E2 levels were positively correlated with the allosuppressive activity of individual clones, suggesting that this molecule may be a useful predictive biomarker for the allosuppressive potential of mesenchymal stromal cells. In contrast, inhibitory studies of indoleamine 2,3 dioxygenase indicated that none of the clones used this enzyme to mediate their allosuppressive effect. Differentiation studies revealed the presence of tripotent, bipotent and unipotent CD271-MSC and PA-MSC clones which suppressed the allogeneic reaction to differing extents in vitro. In conclusion, our findings demonstrate differences between CD271-MSCs and PA-MSCs and indicate that neither proliferation potential nor differentiation potential represents a consistent predictive parameter for the immunomodulatory effects of either type of mesenchymal stromal cells. PMID:23975178

  8. Osteogenic differentiation of CD271+ cells from rabbit bone marrow cultured on three phase PCL/TZ-HA bioactive scaffolds: comparative study with mesenchymal stem cells (MSCs)

    PubMed Central

    Colosimo, Alessia; Rofani, Cristina; Ciraci, Elisa; Salerno, Aurelio; Oliviero, Maria; Maio, Ernesto Di; Iannace, Salvatore; Netti, Paolo A; Velardi, Francesco; Berardi, Anna C

    2015-01-01

    Tissue engineering is one of the major challenges of orthopedics and trauma surgery for bone regeneration. Biomaterials filled with mesenchymal stem cells (MSCs) are considered the most promising approach in bone tissue engineering. Furthermore, our previous study showed that the multi-phase poly [ε-caprolactone]/thermoplastic zein-hydroxyapatite (PCL/TZ-HA) biomaterials improved rabbit (r) MSCs adhesion and osteoblast differentiation, thus demonstrating high potential of this bioengineered scaffold for bone regeneration. In the recent past, CD271 has been applied as a specific selective marker for the enrichment of MSCs from bone marrow (BM-MSCs). In the present study, we aimed at establishing whether CD271-based enrichment could be an efficient method for the selection of rBM-MSCs, displaying higher ability in osteogenic differentiation than non-selected rBM-MSCs in an in vitro system. CD271+ cells were isolated from rabbit bone marrow and were compared with rMSCs in their proliferation rate and osteogenic differentiation capability. Furthermore, rCD271+ cells were tested in their ability to adhere, proliferate and differentiate into osteogenic lineage, while growing on PCL/TZ-HA scaffolds, in comparison to rMSCs. Our result demonstrate that rCD271+ cells were able to adhere, proliferate and differentiate into osteoblasts when cultured on PCL/TZ-HA scaffolds in significantly higher levels as compared to rMSCs. Based on these findings, CD271 marker might serve as an optimal alternative MSCs selection method for the potential preclinical and clinical application of these cells in bone tissue regeneration. PMID:26550238

  9. Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV

    SciTech Connect

    Fukuchi, Mikoto; Wanotayan, Rujira; Liu, Sicheng; Imamichi, Shoji; Sharma, Mukesh Kumar; Matsumoto, Yoshihisa

    2015-06-12

    XRCC4 and DNA Ligase IV (LIG4) cooperate to join two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). However, it is not fully understood how these proteins are localized to the nucleus. Here we created XRCC4{sup K271R} mutant, as Lys271 lies within the putative nuclear localization signal (NLS), and XRCC4{sup K210R} mutant, as Lys210 was reported to undergo SUMOylation, implicated in the nuclear localization of XRCC4. Wild-type and mutated XRCC4 with EGFP tag were introduced into HeLa cell, in which endogenous XRCC4 had been knocked down using siRNA directed to 3′-untranslated region, and tested for the nuclear localization function by fluorescence microscopy. XRCC4{sup K271R} was defective in the nuclear localization of itself and LIG4, whereas XRCC4{sup K210R} was competent for the nuclear localization with LIG4. To examine DSB repair function, wild-type and mutated XRCC4 were introduced into XRCC4-deficient M10. M10-XRCC4{sup K271R}, but not M10-XRCC4{sup K210R}, showed significantly reduced surviving fraction after 2 Gy γ-ray irradiation as compared to M10-XRCC4{sup WT}. The number of γ-H2AX foci remaining 2 h after 2 Gy γ-ray irradiation was significantly greater in M10-XRCC4{sup K271R} than in M10-XRCC4{sup WT}, whereas it was only marginally increased in M10-XRCC4{sup K210R} as compared to M10-XRCC4{sup WT}. The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4. - Highlights: • XRCC4{sup K271R} is defective in the nuclear localization of itself and LIG4. • XRCC4{sup K210R} is competent for the nuclear localization of itself and LIG4. • XRCC4{sup K271R} is deficient in DSB repair function. • XRCC4{sup K210R} is mostly normal in DSB repair function.

  10. Three-dimensional structure of phosphoribosyl pyrophosphate synthetase from E. coli at 2.71 Å resolution

    NASA Astrophysics Data System (ADS)

    Timofeev, V. I.; Abramchik, Yu. A.; Zhukhlistova, N. E.; Muravieva, T. I.; Esipov, R. S.; Kuranova, I. P.

    2016-01-01

    Phosphoribosyl pyrophosphate synthetase from Escherichia coli was cloned, purified, and crystallized. Single crystals of the enzyme were grown under microgravity. The X-ray diffraction data set was collected at the Spring-8 synchrotron facility and used to determine the three-dimensional structure of the enzyme by the molecular-replacement method at 2.71 Å resolution. The active and regulatory sites in the molecule of E. coli phosphoribosyl pyrophosphate synthetase were revealed by comparison with the homologous protein from Bacillus subtilis, the structure of which was determined in a complex with functional ligands. The conformations of polypeptide-chain fragments surrounding and composing the active and regulatory sites were shown to be identical in both proteins.

  11. Efficient aminoacylation of the tRNA(Ala) acceptor stem: dependence on the 2:71 base pair.

    PubMed Central

    Beuning, Penny J; Nagan, Maria C; Cramer, Christopher J; Musier-Forsyth, Karin; Gelpí, Josep-Lluis; Bashford, Donald

    2002-01-01

    Specific aminoacylation by aminoacyl-tRNA synthetases requires accurate recognition of cognate tRNA substrates. In the case of alanyl-tRNA synthetase (AlaRS), RNA duplexes that mimic the acceptor stem of the tRNA are efficient substrates for aminoacylation in vitro. It was previously shown that recognition by AlaRS is severely affected by a simple base pair transversion of the G2:C71 pair at the second position in the RNA helix. In this study, we determined the aminoacylation efficiencies of 50 variants of the tRNA(Ala) acceptor stem containing substitutions at the 2:71 position. We find that there is not a single functional group of the wild-type G2:C71 base pair that is critical for positive recognition. Rather, we observed that base-pair orientation plays an important role in recognition. In particular, pyrimidine2:purine71 combinations generally resulted in decreased aminoacylation efficiency compared to the corresponding purine:pyrimidine pair. Moreover, the activity of a pyrimidine:purine variant could be partially restored by the presence of a major groove amino group at position 71. In an attempt to understand this result further, dielectric continuum electrostatic calculations were carried out, in some cases with additional inclusion of van der Waals interaction energies, to determine interaction potentials of the wild-type duplexAla and seven 2:71 variants. This analysis revealed a positive correlation between major groove negative electrostatic potential in the vicinity of the 3:70 base pair and measured aminoacylation efficiency. PMID:12022232

  12. From COST 271 to 296 EU actions on ionospheric monitoring and modelling for terrestrial and Earth space radio systems

    NASA Astrophysics Data System (ADS)

    Zolesi, B.; Cander, Lj. R.; Altadill, D.

    The ionospheric community has long been aware that co-operative research on an international basis is essential to deal with temporal and spatial changes in the ionosphere that influence the performance of terrestrial and Earth-space radio systems. The EU COST (Co-operation in the field of Scientific and Technical Research) 271 Action on "Effects of the Upper Atmosphere on Terrestrial and Earth-space Communications" has had during the period of October 2000-August 2004 the following main objectives: (1) to evaluate the influence of upper atmospheric conditions on terrestrial and Earth-space communications, (2) to develop methods and techniques to improve ionospheric models over Europe for telecommunication and navigation applications and (3) to transfer the results to the appropriate radiocommunication study groups of the International Telecommunication Union (ITU-R) and other national and international organizations dealing with the modern communication systems. At the beginning of 2005 the new 296 Action in the COST Telecommunications, Information Science and Technology domain on "Mitigation of Ionospheric Effects on Radio Systems (MIERS)" was approved for the period 2005-2009. The main objectives of the MIERS are: (a) to support and enhanced the existing European facilities for historical and real-time digital ionospheric data collection and exchange; (b) to develop an integrated approach to ionospheric modelling, create the mechanism needed to ingest processed data into models, extend and develop suitable mitigation models and define the protocols needed to link models together; and (c) to strengthen the areas of expertise that already exist by stimulating closer cooperation between scientists and users, focusing the scope of all the previous COST ionospheric related studies to the mitigation of ionospheric effects on radio systems. This paper summarises briefly how the major objectives of the COST271 Action have been achieved and what are the most important

  13. Regulation of genotoxic stress response by homeodomain-interacting protein kinase 2 through phosphorylation of cyclic AMP response element-binding protein at serine 271.

    PubMed

    Sakamoto, Kensuke; Huang, Bo-Wen; Iwasaki, Kenta; Hailemariam, Kiros; Ninomiya-Tsuji, Jun; Tsuji, Yoshiaki

    2010-08-15

    CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. Here we found that homeodomain-interacting protein kinase 2 (HIPK2), a DNA-damage responsive nuclear kinase, is a new CREB kinase for phosphorylation at Ser-271 but not Ser-133, and activates CREB transactivation function including brain-derived neurotrophic factor (BDNF) mRNA expression. Ser-271 to Glu-271 substitution potentiated the CREB transactivation function. ChIP assays in SH-SY5Y neuroblastoma cells demonstrated that CREB Ser-271 phosphorylation by HIPK2 increased recruitment of a transcriptional coactivator CBP (CREB binding protein) without modulation of CREB binding to the BDNF CRE sequence. HIPK2-/- MEF cells were more susceptible to apoptosis induced by etoposide, a DNA-damaging agent, than HIPK2+/+ cells. Etoposide activated CRE-dependent transcription in HIPK2+/+ MEF cells but not in HIPK2-/- cells. HIPK2 knockdown in SH-SY5Y cells decreased etoposide-induced BDNF mRNA expression. These results demonstrate that HIPK2 is a new CREB kinase that regulates CREB-dependent transcription in genotoxic stress. PMID:20573984

  14. Sequence homology requirements for transcriptional silencing of 35S transgenes and post-transcriptional silencing of nitrite reductase (trans)genes by the tobacco 271 locus.

    PubMed

    Thierry, D; Vaucheret, H

    1996-12-01

    The transgene locus of the tobacco plant 271 (271 locus) is located on a telomere and consists of multiple copies of a plasmid carrying an NptII marker gene driven by the cauliflower mosaic virus (CaMV) 19S promoter and the leaf-specific nitrite reductase Nii1 cDNA cloned in the antisense orientation under the control of the CaMV 35S promoter. Previous analysis of gene expression in leaves has shown that this locus triggers both post-transcriptional silencing of the host leaf-specific Nii genes and transcriptional silencing of transgenes driven by the 19S or 35S promoter irrespective of their coding sequence and of their location in the genome. In this paper we show that silencing of transgenes carrying Nii1 sequences occurs irrespective of the promoter driving their expression and of their location within the genome. This phenomenon occurs in roots as well as in leaves although root Nii genes share only 84% identity with leaf-specific Nii1 sequences carried by the 271 locus. Conversely, transgenes carrying the bean Nii gene (which shares 76% identity with the tobacco Nii1 gene) escape silencing by the 271 locus. We also show that transgenes driven by the figwort mosaic virus 34S promoter (which shares 63% identity with the 35S promoter) also escape silencing by the 271 locus. Taken together, these results indicate that a high degree of sequence similarity is required between the sequences of the silencing locus and of the target (trans)genes for both transcriptional and post-transcriptional silencing. PMID:9002606

  15. Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.

    PubMed

    Schafer, Peter H; Truzzi, Francesca; Parton, Anastasia; Wu, Lei; Kosek, Jolanta; Zhang, Ling-Hua; Horan, Gerald; Saltari, Annalisa; Quadri, Marika; Lotti, Roberta; Marconi, Alessandra; Pincelli, Carlo

    2016-07-01

    Phosphodiesterases 4 (PDE4) act as proinflammatory enzymes via degradation of cAMP, whereas PDE4 inhibitors play an anti-inflammatory role in vitro and in vivo. In particular, apremilast has been recently approved for the treatment of psoriasis and psoriatic arthritis. However, little is known on the expression pattern of PDE4 in psoriasis. We report that PDE4B and PDE4D mRNA are overexpressed in peripheral blood mononuclear cells (PBMC) from psoriasis, as compared with normal controls, while apremilast reduces PBMC production of a number of pro-inflammatory cytokines and increases the levels of anti-inflammatory mediators. PDE4 expression is up-regulated in psoriatic dermis as compared with normal skin, with particular regard to fibroblasts. This is confirmed in vitro, where both dermal fibroblasts (DF) and, to a greater extent, myofibroblasts (DM) express all PDE4 isoforms at the mRNA and protein level. Because PDE4 interacts with the nerve growth factor (NGF) receptor CD271 in lung fibroblasts, we evaluated the relationship and function of PDE4 and CD271 in normal human skin fibroblasts. All PDE4 isoforms co-immunoprecipitate with CD271 in DM, while apremilast inhibits apoptosis induced by β-amyloid, a CD271 ligand, in DM. Furthermore, apremilast significantly reduces NGF- and transforming growth factor-β1 (TGF-β1)-induced fibroblast migration, and inhibits DF differentiation into DM mediated by NGF or TGF-β1. Finally, in DM, apremilast significantly reduces cAMP degradation induced by treatment with β-amyloid. Taken together, these results indicate that PDE4 play an important role in psoriasis. In addition, the study reveals that the PDE4/CD271 complex could be important in modulating fibroblast functions. PMID:26806620

  16. Design of 9.271-pressure-ratio 5-stage core compressor and overall performance for first 3 stages

    NASA Technical Reports Server (NTRS)

    Steinke, Ronald J.

    1986-01-01

    Overall aerodynamic design information is given for all five stages of an axial flow core compressor (74A) having a 9.271 pressure ratio and 29.710 kg/sec flow. For the inlet stage group (first three stages), detailed blade element design information and experimental overall performance are given. At rotor 1 inlet tip speed was 430.291 m/sec, and hub to tip radius ratio was 0.488. A low number of blades per row was achieved by the use of low-aspect-ratio blading of moderate solidity. The high reaction stages have about equal energy addition. Radial energy varied to give constant total pressure at the rotor exit. The blade element profile and shock losses and the incidence and deviation angles were based on relevant experimental data. Blade shapes are mostly double circular arc. Analysis by a three-dimensional Euler code verified the experimentally measured high flow at design speed and IGV-stator setting angles. An optimization code gave an optimal IGV-stator reset schedule for higher measured efficiency at all speeds.

  17. Early maladaptive schema factors, chronic pain and depressiveness: a study with 271 chronic pain patients and 331 control participants.

    PubMed

    Saariaho, Tom; Saariaho, Anita; Karila, Irma; Joukamaa, Matti

    2012-01-01

    Chronic pain and depression are coexisting entities with high simultaneous prevalence. Both are linked with early adversities. Early maladaptive schemas (EMS) can be seen as a reflection of these adversities. EMSs extensively indicate underlying psychic patterns and provide a good opportunity to detect covert processes and psychic shapes (latent factors), which create the basis of how people rate their schemas. The purpose of this study was to explore these latent, higher order schema factors (SF) and to find out how they are associated with pain intensity or depression in chronic pain patients and a control sample. The study subjects consisted of 271 first-visit pain patients and 331 control participants. Sociodemographic and pain data were gathered by questionnaire; 18 EMSs were measured with the Young Schema Questionnaire (short form) and depressiveness was measured with the Beck Depression Inventory, Version II. Exploratory factor and regression analyses were used. The chronic pain patient group showed two SFs. The first SF showed a shameful, defective, socially isolated, failure, emotionally inhibited, deprived, submissive and resigned pattern. The second SF showed a demanding, approval seeking, self-sacrificing and punitive pattern. SF1 predicted more than half of the depressiveness in the pain patient sample. A three-factor structure was found in the control sample, and SFs 1 and 3 together predicted almost one-third of depressiveness. The pain patient and the control groups had a different, higher order factor structure. We assume that SF1 in the pain patients reflected a rather serious, undefined early psychic trauma and was also associated with their depressiveness. PMID:21210495

  18. Coexpression of SOX10/CD271 (p75NTR) and β-Galactosidase in Large to Giant Congenital Melanocytic Nevi of Pediatric Patients

    PubMed Central

    Barysch, Marjam J.; Levesque, Mitchell P.; Cheng, Phil; Karpova, Maria B.; Mihic-Probst, Daniela; Civenni, Gianluca; Shakhova, Olga; Sommer, Lukas; Biedermann, Thomas; Schiestl, Clemens; Dummer, Reinhard

    2014-01-01

    Background Congenital melanocytic nevi (CMNs) are melanocytic neoplasms that can transform into melanoma. However, this development is impeded in the majority of cases and mostly affects patients with large or giant CMNs. Methods To elucidate mechanisms that keep CMNs from malignant transformation, CMN tissue biopsies were investigated for p-ERK and senescence markers by immunohistochemistry and for SOX10/CD271 (p75NTR) by immunofluorescence. CMN cells were cultivated, and MTT assays were performed for evaluating cell viability. Mutation status for NRAS and BRAF was performed by real-time PCR. Results 13 CMNs (from patients aged 0.5-11.8 years, mean: 2.7) showed immunoreactivity for SOX10/CD271 (p75NTR) in 34.2%. p-ERK was immunoreactive in 80% (4/5); β-galactosidase was significantly stronger expressed in CMNs compared to melanocytic nevi of patients over 70 years (p = 0.0085). The 5 CMN cultures were immunoreactive for SOX10/CD271 (p75NTR) in 36.7%. By silencing SOX10 by siRNA in 2 CMN cell cultures, cell viability decreased significantly. NRASQ61K mutation was found in 91.7% (11/12) and BRAFV600E in 6.3% of all analyzable CMNs (1/16). Conclusions Oncogene-induced senescence might prevent malignant transformation through activation of the mitogen-activated protein kinase pathway. SOX10 is necessary for the viability of human CMN cell cultures and may be responsible for clinical changes during aging. PMID:27047921

  19. Genes for split hand/split foot and laterality defects on 7q22.1 and Xq24-q27.1

    SciTech Connect

    Genuardi, M.; Gurrieri, F.; Neri, G.

    1994-03-01

    Koiffman et al recently reported on a boy with split hand/split foot (SHSF), situs viscerum inversus, polysplenia, and other abnormalities, who also had an apparently balanced 7;8 rearrangement with breakpoint on 7q22. This case presents many similarities with a member of a family cosegregating SHSF and a 2;7 balanced reciprocal translocation involving band 7q22.1 recently identified. This subject, who could not be studied cytogenetically, had SHSF and a laterality sequence comprised in the spectrum of the polyasplenia developmental field defect or the Ivemark syndrome. The authors made the hypothesis that both SHSF and the laterality defects in this patient could be ascribed to the disruption of a single pleiotropic gene or involvement of contiguous genes located on 7q22.1. The observation of Koiffmann et al. lends support to this hypothesis and renders 7q22.1 an extremely important region for the study of pattern formation and blastogenesis. It is also intriguing that mutations responsible for X-linked SHSF, ventral midline defects, and visceral heterotaxy have been mapped within the same region of the X chromosome, that is, X-24-q27.1. Altogether these observations suggest that homologous clusters of contiguous genes or homologous single pleiotropic genes may be located on 7q22.1 and Xq24-q27.1.

  20. Corrective Action Decision Document for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems, Nevada Test Site, Nevada, Rev. 0

    SciTech Connect

    NNSA /NV

    2002-09-16

    This corrective action decision document (CADD) identifies and rationalizes the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's selection of a recommended corrective action alternative (CAA) appropriate to facilitate the closure of Corrective Action Unit (CAU) 271, Areas 25, 26, and 27 Septic Systems, Nevada Test Site (NTS), Nevada, under the Federal Facility Agreement and Consent Order (FFACO). Located on the NTS approximately 65 miles northwest of Las Vegas, CAU 271 consists of fifteen Corrective Action Sites (CASs). The CASs consist of 13 septic systems, a radioactive leachfield, and a contaminated reservoir. The purpose of this CADD is to identify and provide a rationale for the selection of a recommended CAA for each CAS within CAU 271. Corrective action investigation (CAI) activities were performed from October 29, 2001, through February 22, 2002, and April 29, 2002, through June 25, 2002. Analytes detected during the CAI were evaluated against preliminary action levels and regulatory disposal limits to determine contaminants of concern (COC) for each CAS. It was determined that contaminants of concern included hydrocarbon-contaminated media, polychlorinated biphenyls, and radiologically-contaminated media. Three corrective action objectives were identified for these CASs, and subsequently three CAAs developed for consideration based on a review of existing data, future use, and current operations in Areas 25, 26, and 27 of the NTS. These CAAs were: Alternative 1 - No Further Action, Alternative 2 - Clean Closure, and Alternative 3 - Closure in Place with Administrative Controls. Alternative 2, Clean Closure, was chosen as the preferred CAA for all but two of the CASs (25-04-04 and 27-05-02) because Nevada Administrative Control 444.818 requires clean closure of the septic tanks involved with these CASs. Alternative 3, Closure in Place, was chosen for the final two CASs because the short-term risks of

  1. Corrective Action Investigation Plan for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems, Nevada Test Site, Nevada (Rev. 0, April 2001)

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office

    2001-04-09

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 271 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 271 consists of 15 Corrective Action Sites (CASs) including: thirteen Septic Systems (25-04-01, 25-04-03, 25-04-04, 25-04-08, 25-04-09, 25-04-10, 25-04-11, 26-04-01, 26-04-02, 26-05-03, 26-05-04, 26-05-05, and 27-05-02), one Contaminated Water Reservoir (26-03-01), and one Radioactive Leachfield (26-05-01). The CASs addressed by CAU 271 are located at Guard Station 500, the Reactor Control Point (RCP), Bare Reactor Experiment - Nevada Tower, and Engine Test State-1 (ETS-1) facilities in Area 25; the Port Gaston and Project Pluto facilities in Area 26; and the Baker Site in Area 27 of the Nevada Test Site. Between 1 958 and 1973, the RCP and ETS-1 facilities supported the development and testing of nuclear reactors for space propulsion as part of the Nuclear Rocket Development Station. The Project Pluto facilities supported nuclear reactor testing for use as a ramjet propulsion system between 1961 and 1964, followed by similar use for other projects through the early 1980s. The Baker Site facilities were constructed in the 1960s to serve as the staging point where the manufactured components of nuclear devices were assembled, disassembled, and modified. The scope of the investigation strategy at these sites will involve biased and random soil sampling in leachfields using excavation (with drilling as a contingency), collection of soil samples underlying the base of proximal and distal ends of septic tanks and distal ends of distribution structures, defining the lateral and vertical extent of contamination through discrete field and possible stepout location sampling, collection system line

  2. Closure Report for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems, Nevada Test Site, Nevada with Errata Sheet, Revision 0

    SciTech Connect

    Mark Krauss

    2004-08-01

    The purpose of this CR is to document that closure activities have met the approved closure standards detailed in the NDEP-approved CAP for CAU 271. The purpose of the Errata Sheet is as follows: In Appendix G, Use Restriction (UR) Documentation, the UR form and drawing of the UR area do not reflect the correct coordinates. Since the original UR was put into place, the UR Form has been updated to include additional information that was not on the original form. This Errata Sheet replaces the original UR Form and drawing. In place of the drawing of the UR area, an aerial photograph is included which reflects the UR area and the correct coordinates for the UR area.

  3. Generation of 1.5 W average power, 18 kHz repetition rate coherent mid-ultraviolet radiation at 271.2 nm.

    PubMed

    Biswal, Ramakanta; Agrawal, Praveen K; Dixit, Sudhir K; Nakhe, Shankar V

    2015-11-10

    This paper presents to our knowledge a first time study on the generation of 1.5 W average power, 18 kHz repetition rate coherent mid-ultraviolet (UV) radiation at 271.2 nm. The work is based on frequency summing of coherent green (G: 510.6 nm) and yellow (Y: 578.2 nm) radiations of a copper-HBr laser in a β-barium borate crystal. Average and peak sum frequency conversion efficiencies of about 13% and 16%, respectively, are obtained. The sum frequency results are experimentally analyzed in terms of the extent of matching of green and yellow pump radiations in space, time, and frequency domains. The result is of high significance for many applications in photonics components fabrication, semiconductor technology, and spectroscopy. PMID:26560794

  4. Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H271.

    PubMed

    Marthaler, Adele G; Schmid, Benjamin; Tubsuwan, Alisa; Poulsen, Ulla B; Engelbrecht, Alexander F; Mau-Holzmann, Ulrike A; Hyttel, Poul; Nielsen, Jørgen E; Nielsen, Troels T; Holst, Bjørn

    2016-01-01

    Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease primarily affecting the cerebellum. Very little is known about the molecular mechanisms underlying the disease and, to date, no cure or treatment is available. We have successfully generated bona fide induced pluripotent stem cell (iPSC) lines of SCA2 patients in order to study a disease-specific phenotype. Here, we demonstrate the gene correction of the iPSC line H271 clone 1 where we have exchanged the expanded CAG repeat of the ATXN2 gene with the normal length found in healthy alleles. This gene corrected cell line will provide the ideal control to model SCA2 by iPSC technology. PMID:27345809

  5. Performance Characterization of Loctite (Registered Trademark) 242 and 271 Liquid Locking Compounds (LLCs) as a Secondary Locking Feature for International Space Station (ISS) Fasteners

    NASA Technical Reports Server (NTRS)

    Dube, Michael J.; Gamwell, Wayne R.

    2011-01-01

    Several International Space Station (ISS) hardware components use Loctite (and other polymer based liquid locking compounds (LLCs)) as a means of meeting the secondary (redundant) locking feature requirement for fasteners. The primary locking method is the fastener preload, with the application of the Loctite compound which when cured is intended to resist preload reduction. The reliability of these compounds has been questioned due to a number of failures during ground testing. The ISS Program Manager requested the NASA Engineering and Safety Center (NESC) to characterize and quantify sensitivities of Loctite being used as a secondary locking feature. The findings and recommendations provided in this investigation apply to the anaerobic LLCs Loctite 242 and 271. No other anaerobic LLCs were evaluated for this investigation. This document contains the findings and recommendations of the NESC investigation

  6. Observation of the 3n evaporation channel in the complete hot-fusion reaction 26Mg + 248Cm leading to the new superheavy nuclide 271Hs.

    PubMed

    Dvorak, J; Brüchle, W; Chelnokov, M; Düllmann, Ch E; Dvorakova, Z; Eberhardt, K; Jäger, E; Krücken, R; Kuznetsov, A; Nagame, Y; Nebel, F; Nishio, K; Perego, R; Qin, Z; Schädel, M; Schausten, B; Schimpf, E; Schuber, R; Semchenkov, A; Thörle, P; Türler, A; Wegrzecki, M; Wierczinski, B; Yakushev, A; Yeremin, A

    2008-04-01

    The analysis of a large body of heavy ion fusion reaction data with medium-heavy projectiles (6 < or = Z < or = 18) and actinide targets suggests a disappearance of the 3n exit channel with increasing atomic number of the projectile. Here, we report a measurement of the excitation function of the reaction (248)Cm ((26)Mg,xn)(274-x)Hs and the observation of the new nuclide (271)Hs produced in the 3n evaporation channel at a beam energy well below the Bass fusion barrier with a cross section comparable to the maxima of the 4n and 5n channels. This indicates the possible discovery of new neutron-rich transactinide nuclei using relatively light heavy ion beams of the most neutron-rich stable isotopes and actinide targets. PMID:18517941

  7. An interstitial deletion-insertion involving chromosomes 2p25.3 and Xq27.1, near SOX3, causes X-linked recessive hypoparathyroidism

    PubMed Central

    Bowl, Michael R.; Nesbit, M. Andrew; Harding, Brian; Levy, Elaine; Jefferson, Andrew; Volpi, Emanuela; Rizzoti, Karine; Lovell-Badge, Robin; Schlessinger, David; Whyte, Michael P.; Thakker, Rajesh V.

    2005-01-01

    X-linked recessive hypoparathyroidism, due to parathyroid agenesis, has been mapped to a 906-kb region on Xq27 that contains 3 genes (ATP11C, U7snRNA, and SOX3), and analyses have not revealed mutations. We therefore characterized this region by combined analysis of single nucleotide polymorphisms and sequence-tagged sites. This identified a 23- to 25-kb deletion, which did not contain genes. However, DNA fiber–FISH and pulsed-field gel electrophoresis revealed an approximately 340-kb insertion that replaced the deleted fragment. Use of flow-sorted X chromosome–specific libraries and DNA sequence analyses revealed that the telomeric and centromeric breakpoints on X were, respectively, approximately 67 kb downstream of SOX3 and within a repetitive sequence. Use of a monochromosomal somatic cell hybrid panel and metaphase-FISH mapping demonstrated that the insertion originated from 2p25 and contained a segment of the SNTG2 gene that lacked an open reading frame. However, the deletion-insertion [del(X)(q27.1) inv ins (X;2)(q27.1;p25.3)], which represents a novel abnormality causing hypoparathyroidism, could result in a position effect on SOX3 expression. Indeed, SOX3 expression was demonstrated, by in situ hybridization, in the developing parathyroid tissue of mouse embryos between 10.5 and 15.5 days post coitum. Thus, our results indicate a likely new role for SOX3 in the embryonic development of the parathyroid glands. PMID:16167084

  8. Identification of Tetrapeptides from a Mixture Based Positional Scanning Library That Can Restore nM Full Agonist Function of the L106P, I69T, I102S, A219V, C271Y, and C271R Human Melanocortin-4 Polymorphic Receptors (hMC4Rs)

    PubMed Central

    2015-01-01

    Human obesity has been linked to genetic factors and single nucleotide polymorphisms (SNPs). Melanocortin-4 receptor (MC4R) SNPs have been associated with up to 6% frequency in morbidly obese children and adults. A potential therapy for individuals possessing such genetic modifications is the identification of molecules that can restore proper receptor signaling and function. These compounds could serve as personalized medications improving quality of life issues as well as alleviating diseases symptoms associated with obesity including type 2 diabetes. Several hMC4 SNP receptors have been pharmacologically characterized in vitro to have a decreased, or a lack of response, to endogenous agonists such as α-, β-, and γ2-melanocyte stimulating hormones (MSH) and adrenocorticotropin hormone (ACTH). Herein we report the use of a mixture based positional scanning combinatorial tetrapeptide library to discover molecules with nM full agonist potency and efficacy to the L106P, I69T, I102S, A219V, C271Y, and C271R hMC4Rs. The most potent compounds at all these hMC4R SNPs include Ac-His-(pI)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Arg-(pI)Phe-NH2, and Ac-Arg-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, revealing new ligand pharmacophore models for melanocortin receptor drug design strategies. PMID:24517312

  9. Glioma invasion mediated by the p75 neurotrophin receptor (p75NTR/CD271) requires regulated interaction with PDLIM1

    PubMed Central

    Ahn, B Y; Saldanha-Gama, R F G; Rahn, J J; Hao, X; Zhang, J; Dang, N-H; Alshehri, M; Robbins, S M; Senger, D L

    2016-01-01

    The invasive nature of glioblastoma renders them incurable by current therapeutic interventions. Using a novel invasive human glioma model, we previously identified the neurotrophin receptor p75NTR (aka CD271) as a mediator of glioma invasion. Herein, we provide evidence that preventing phosphorylation of p75NTR on S303 by pharmacological inhibition of PKA, or by a mutational strategy (S303G), cripples p75NTR-mediated glioma invasion resulting in serine phosphorylation within the C-terminal PDZ-binding motif (SPV) of p75NTR. Consistent with this, deletion (ΔSPV) or mutation (SPM) of the PDZ motif results in abrogation of p75NTR-mediated invasion. Using a peptide-based strategy, we identified PDLIM1 as a novel signaling adaptor for p75NTR and provide the first evidence for a regulated interaction via S425 phosphorylation. Importantly, PDLIM1 was shown to interact with p75NTR in highly invasive patient-derived glioma stem cells/tumor-initiating cells and shRNA knockdown of PDLIM1 in vitro and in vivo results in complete ablation of p75NTR-mediated invasion. Collectively, these data demonstrate a requirement for a regulated interaction of p75NTR with PDLIM1 and suggest that targeting either the PDZ domain interactions and/or the phosphorylation of p75NTR by PKA could provide therapeutic strategies for patients with glioblastoma. PMID:26119933

  10. Long-term posttraumatic stress symptoms among 3,271 civilian survivors of the September 11, 2001, terrorist attacks on the World Trade Center.

    PubMed

    DiGrande, Laura; Neria, Yuval; Brackbill, Robert M; Pulliam, Paul; Galea, Sandro

    2011-02-01

    Although the September 11, 2001, terrorist attacks were the largest human-made disaster in US history, there is little extant research documenting the attacks' consequences among those most directly affected, that is, persons who were in the World Trade Center towers. Data from a cross-sectional survey conducted 2-3 years after the attacks ascertained the prevalence of long-term, disaster-related posttraumatic stress symptoms and probable posttraumatic stress disorder (PTSD) in 3,271 civilians who evacuated World Trade Center towers 1 and 2. Overall, 95.6% of survivors reported at least 1 current posttraumatic stress symptom. The authors estimated the probable rate of PTSD at 15.0% by using the PTSD Checklist. Women and minorities were at an increased risk of PTSD. A strong inverse relation with annual income was observed. Five characteristics of direct exposure to the terrorist attacks independently predicted PTSD: being on a high floor in the towers, initiating evacuation late, being caught in the dust cloud that resulted from the tower collapses, personally witnessing horror, and sustaining an injury. Working for an employer that sustained fatalities also increased risk. Each addition of an experience of direct exposure resulted in a 2-fold increase in the risk of PTSD (odds ratio = 2.09, 95% confidence interval: 1.84, 2.36). Identification of these risk factors may be useful when screening survivors of large-scale terrorist events for long-term psychological sequelae. PMID:21190987

  11. Glioma invasion mediated by the p75 neurotrophin receptor (p75(NTR)/CD271) requires regulated interaction with PDLIM1.

    PubMed

    Ahn, B Y; Saldanha-Gama, R F G; Rahn, J J; Hao, X; Zhang, J; Dang, N-H; Alshehri, M; Robbins, S M; Senger, D L

    2016-03-17

    The invasive nature of glioblastoma renders them incurable by current therapeutic interventions. Using a novel invasive human glioma model, we previously identified the neurotrophin receptor p75(NTR) (aka CD271) as a mediator of glioma invasion. Herein, we provide evidence that preventing phosphorylation of p75(NTR) on S303 by pharmacological inhibition of PKA, or by a mutational strategy (S303G), cripples p75(NTR)-mediated glioma invasion resulting in serine phosphorylation within the C-terminal PDZ-binding motif (SPV) of p75(NTR). Consistent with this, deletion (ΔSPV) or mutation (SPM) of the PDZ motif results in abrogation of p75(NTR)-mediated invasion. Using a peptide-based strategy, we identified PDLIM1 as a novel signaling adaptor for p75(NTR) and provide the first evidence for a regulated interaction via S425 phosphorylation. Importantly, PDLIM1 was shown to interact with p75(NTR) in highly invasive patient-derived glioma stem cells/tumor-initiating cells and shRNA knockdown of PDLIM1 in vitro and in vivo results in complete ablation of p75(NTR)-mediated invasion. Collectively, these data demonstrate a requirement for a regulated interaction of p75(NTR) with PDLIM1 and suggest that targeting either the PDZ domain interactions and/or the phosphorylation of p75(NTR) by PKA could provide therapeutic strategies for patients with glioblastoma. PMID:26119933

  12. Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem cell antigen-1

    PubMed Central

    Battula, Venkata Lokesh; Treml, Sabrina; Bareiss, Petra M.; Gieseke, Friederike; Roelofs, Helene; de Zwart, Peter; Müller, Ingo; Schewe, Bernhard; Skutella, Thomas; Fibbe, Willem E.; Kanz, Lothar; Bühring, Hans-Jörg

    2009-01-01

    Background Conventionally, mesenchymal stem cells are functionally isolated from primary tissue based on their capacity to adhere to a plastic surface. This isolation procedure is hampered by the unpredictable influence of co-cultured hematopoietic and/or other unrelated cells and/or by the elimination of a late adhering mesenchymal stem cells subset during removal of undesired cells. To circumvent these limitations, several antibodies have been developed to facilitate the prospective isolation of mesenchymal stem cells. Recently, we described a panel of monoclonal antibodies with superior selectivity for mesenchymal stem cells, including the monoclonal antibodies W8B2 against human mesenchymal stem cell antigen-1 (MSCA-1) and 39D5 against a CD56 epitope, which is not expressed on natural killer cells. Design and Methods Bone marrow derived mesenchymal stem cells from healthy donors were analyzed and isolated by flow cytometry using a large panel of antibodies against surface antigens including CD271, MSCA-1, and CD56. The growth of mesenchymal stem cells was monitored by colony formation unit fibroblast (CFU-F) assays. The differentiation of mesenchymal stem cells into defined lineages was induced by culture in appropriate media and verified by immunostaining. Results Multicolor cell sorting and CFU-F assays showed that mesenchymal stem cells were ~90-fold enriched in the MSCA-1+CD56− fraction and ~180-fold in the MSCA-1+CD56+ fraction. Phenotype analysis revealed that the expression of CD10, CD26, CD106, and CD146 was restricted to the MSCA-1+CD56− mesenchymal stem cells subset and CD166 to MSCA-1+CD56± mesenchymal stem cells. Further differentiation of these subsets showed that chondrocytes and pancreatic-like islets were predominantly derived from MSCA-1+CD56± cells whereas adipocytes emerged exclusively from MSCA-1+CD56− cells. The culture of single sorted MSCA-1+CD56+ cells resulted in the appearance of phenotypically heterogeneous clones with

  13. The effects of kisspeptin agonist canine KP-10 and kisspeptin antagonist p271 on plasma LH concentrations during different stages of the estrous cycle and anestrus in the bitch.

    PubMed

    Albers-Wolthers, C H J; de Gier, J; Rutten, V P M G; van Kooten, P J S; Leegwater, P A J; Schaefers-Okkens, A C; Kooistra, H S

    2016-07-15

    Kisspeptin (KP) plays a key role in the regulation of the hypothalamic-pituitary-gonadal axis via the release of GnRH. As normal KP signaling is essential for reproductive function, it could be an interesting new target for therapeutic interventions, e.g., nonsurgical contraception in dogs. The aims of the present study were to investigate the effect of KP-10 administration on plasma LH concentration in different stages of the reproductive cycle and to investigate the suitability of p271 as KP antagonist in the bitch. Two groups of six adult Beagle bitches were used. In one group, plasma LH concentration was determined before (40 and 0 minutes) and 10, 20, 40, and 60 minutes after the intravenous administration of 0.5-μg/kg body weight (BW) canine KP-10. In the other group, the bitches received a continuous intravenous infusion with p271 (50 μg/kg BW/h) for 3 hours, and 0.5-μg/kg BW canine KP-10 was administered intravenously 2 hours after the start of the p271 infusion. Their plasma LH concentration was determined before (-40 and 0 minutes) and 30, 60, 90, 120, 130, 140, 160, and 180 minutes after the start of the p271 infusion. In both groups, the experiments were performed during the follicular phase, the first and second half of the luteal phase, and during anestrus. Canine KP-10 induced an increase of plasma LH concentration during all estrous cycle stages and anestrus. There was no difference in LH response between the two groups. The lowest LH response was seen during the follicular phase and the highest response during anestrus. The area under the curve (AUC) for LH and LH increment in the follicular phase were lower than those in anestrus. The AUC LH and LH increment in the first half of the luteal phase were lower than those in the second half of the luteal phase and anestrus. The AUC LH and LH increment in the second half of the luteal phase were not different from those in anestrus. Continuous administration of the antagonist p271 did not alter

  14. Addendum to the Closure Report for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Lynn Kidman

    2008-10-01

    This document constitutes an addendum to the August 2004, Closure Report for Corrective Action Unit 271, Areas 25, 26, and 27 Septic Systems as described in the document Recommendations and Justifications for Modifications for Use Restrictions Established under the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office Federal Facility Agreement and Consent Order (UR Modification document) dated February 2008. The UR Modification document was approved by NDEP on February 26, 2008. The approval of the UR Modification document constituted approval of each of the recommended UR modifications. In conformance with the UR Modification document, this addendum consists of: • This cover page that refers the reader to the UR Modification document for additional information • The cover and signature pages of the UR Modification document • The NDEP approval letter • The corresponding section of the UR Modification document This addendum provides the documentation justifying the modification of the UR for CAS 27-05-02, Leachfield. This UR was established as part of a Federal Facility Agreement and Consent Order (FFACO) corrective action and is based on the presence of contaminants at concentrations greater than the action levels established at the time of the initial investigation (FFACO, 1996; as amended August 2006). Since this UR was established, practices and procedures relating to the implementation of risk-based corrective actions (RBCA) have changed. Therefore, this UR was re-evaluated against the current RBCA criteria as defined in the Industrial Sites Project Establishment of Final Action Levels (NNSA/NSO, 2006c). This re-evaluation consisted of comparing the original data (used to define the need for the UR) to risk-based final action levels (FALs) developed using the current Industrial Sites RBCA process. The re-evaluation resulted in a recommendation to modify the UR to an administrative UR. Administrative URs differ from

  15. Observation of the 3n Evaporation Channel in the Complete Hot-Fusion Reaction {sup 26}Mg+{sup 248}Cm Leading to the New Superheavy Nuclide {sup 271}Hs

    SciTech Connect

    Dvorak, J.; Dvorakova, Z.; Kruecken, R.; Nebel, F.; Perego, R.; Schuber, R.; Tuerler, A.; Wierczinski, B.; Yakushev, A.; Bruechle, W.; Jaeger, E.; Schaedel, M.; Schausten, B.; Schimpf, E.; Chelnokov, M.; Kuznetsov, A.; Yeremin, A.; Duellmann, Ch. E.; Eberhardt, K.; Nagame, Y.

    2008-04-04

    The analysis of a large body of heavy ion fusion reaction data with medium-heavy projectiles (6{<=}Z{<=}18) and actinide targets suggests a disappearance of the 3n exit channel with increasing atomic number of the projectile. Here, we report a measurement of the excitation function of the reaction {sup 248}Cm({sup 26}Mg,xn){sup 274-x}Hs and the observation of the new nuclide {sup 271}Hs produced in the 3n evaporation channel at a beam energy well below the Bass fusion barrier with a cross section comparable to the maxima of the 4n and 5n channels. This indicates the possible discovery of new neutron-rich transactinide nuclei using relatively light heavy ion beams of the most neutron-rich stable isotopes and actinide targets.

  16. 38 CFR 17.271 - Eligibility.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Health and Medical Program of the Department of Veterans Affairs (champva)-Medical Care for Survivors and... of education must remain eligible for medical care until: (i) The end of the six-month period... Medicare Part B, are eligible for CHAMPVA as secondary payer to Medicare Part A and any other...

  17. 38 CFR 17.271 - Eligibility.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Health and Medical Program of the Department of Veterans Affairs (champva)-Medical Care for Survivors and... of education must remain eligible for medical care until: (i) The end of the six-month period... Medicare Part B, are eligible for CHAMPVA as secondary payer to Medicare Part A and any other...

  18. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the facility, to personnel of the vessel while in drydock or on a marine railway, sanitary facilities... prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor...

  19. 40 CFR 408.271 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Steamed and Canned Oyster... this chapter shall apply to this subpart. (b) The term product shall mean the weight of the oyster...

  20. 40 CFR 408.271 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Steamed and Canned Oyster... this chapter shall apply to this subpart. (b) The term product shall mean the weight of the oyster...

  1. 40 CFR 408.271 - Specialized definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Steamed and Canned Oyster... this chapter shall apply to this subpart. (b) The term product shall mean the weight of the oyster...

  2. 40 CFR 408.271 - Specialized definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Steamed and Canned Oyster... this chapter shall apply to this subpart. (b) The term product shall mean the weight of the oyster...

  3. 40 CFR 408.271 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Steamed and Canned Oyster... this chapter shall apply to this subpart. (b) The term product shall mean the weight of the oyster...

  4. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces and..., dampers, etc.) used to capture or transport particulate matter generated by an electric arc furnace or AOD... materials into the top of an electric arc furnace or the addition of molten steel or other materials...

  5. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces and..., dampers, etc.) used to capture or transport particulate matter generated by an electric arc furnace or AOD... materials into the top of an electric arc furnace or the addition of molten steel or other materials...

  6. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces... this part. (a) Electric arc furnace (EAF) means a furnace that produces molten steel and heats the charge materials with electric arcs from carbon electrodes. Furnaces that continuously feed...

  7. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces... this part. (a) Electric arc furnace (EAF) means a furnace that produces molten steel and heats the charge materials with electric arcs from carbon electrodes. Furnaces that continuously feed...

  8. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces and..., dampers, etc.) used to capture or transport particulate matter generated by an electric arc furnace or AOD... materials into the top of an electric arc furnace or the addition of molten steel or other materials...

  9. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces... this part. (a) Electric arc furnace (EAF) means a furnace that produces molten steel and heats the charge materials with electric arcs from carbon electrodes. Furnaces that continuously feed...

  10. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces... this part. (a) Electric arc furnace (EAF) means a furnace that produces molten steel and heats the charge materials with electric arcs from carbon electrodes. Furnaces that continuously feed...

  11. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces... this part. (a) Electric arc furnace (EAF) means a furnace that produces molten steel and heats the charge materials with electric arcs from carbon electrodes. Furnaces that continuously feed...

  12. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces and..., dampers, etc.) used to capture or transport particulate matter generated by an electric arc furnace or AOD... materials into the top of an electric arc furnace or the addition of molten steel or other materials...

  13. 40 CFR 60.271a - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Steel Plants: Electric Arc Furnaces and..., dampers, etc.) used to capture or transport particulate matter generated by an electric arc furnace or AOD... materials into the top of an electric arc furnace or the addition of molten steel or other materials...

  14. 16 CFR 1500.271 - Bonds.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... or any part thereof upon demand of the collector of customs and containing a provision for the... collector of customs. (b) The collector of customs may cancel the liability for liquidated damages incurred... appropriate under the law and in view of the circumstances, but the collector shall not act under...

  15. 16 CFR 1500.271 - Bonds.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... or any part thereof upon demand of the collector of customs and containing a provision for the... collector of customs. (b) The collector of customs may cancel the liability for liquidated damages incurred... appropriate under the law and in view of the circumstances, but the collector shall not act under...

  16. 16 CFR 1500.271 - Bonds.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... or any part thereof upon demand of the collector of customs and containing a provision for the... collector of customs. (b) The collector of customs may cancel the liability for liquidated damages incurred... appropriate under the law and in view of the circumstances, but the collector shall not act under...

  17. 16 CFR 1500.271 - Bonds.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... or any part thereof upon demand of the collector of customs and containing a provision for the... collector of customs. (b) The collector of customs may cancel the liability for liquidated damages incurred... appropriate under the law and in view of the circumstances, but the collector shall not act under...

  18. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... have a reliable source of suitable primary power, either from a power distribution system or locally... communications or reliable communications (at least a landline telephone) from the airport to the nearest Federal... reliable communications (at least a landline telephone) from the airport to the nearest Federal...

  19. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... place will be evaluated: (1) For costs attributable to the carrier's flying operations (direct expenses... altitude at which the carrier must fly to the designated hub; and (v) Other operational elements...

  20. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture...

  1. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture...

  2. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture...

  3. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals.... (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium, inclusive. (c) The term mischmetal refers to a rare earth metal alloy comprised of the natural mixture...

  4. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... set out in section 5 of the Act of August 7, 1961 (75 Stat. 284), development and management of the... use, understanding and enjoyment of its natural, cultural and scientific features. The regulations in..., Orleans and Chatham; and (2) to promote preservation and development, in accordance with the purposes...

  5. 48 CFR 1352.271-77 - Delays.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... in 48 CFR 1371.108, insert the following clause: Delays (APR 2010) When, during the performance of... actions of the Government respecting stoppage of work to permit shifting the vessel, stoppage of hot...

  6. 48 CFR 1352.271-77 - Delays.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... in 48 CFR 1371.108, insert the following clause: Delays (APR 2010) When, during the performance of... actions of the Government respecting stoppage of work to permit shifting the vessel, stoppage of hot...

  7. AB271. Sexual dysfunction in chronic prostatitis

    PubMed Central

    Cho, In-Rae

    2016-01-01

    Chronic prostatitis/ chronic pelvic pain syndrome (CP/CPPS), or NIH category III prostatitis, is a clinical syndrome characterized by genital/ pelvic pain and lower urinary tract symptoms in the absence of urinary tract infection. CPPS is the most common prostatic disease in men younger than 50 years of age and the third most common in men older than 50 years of age. CP/CPPS is a complex entity with unclear etiology. Many articles reported that the high percentage of patients with CP/CPPS had sexual dysfunction. The most common symptoms of sexual dysfunction in chronic prostatitis patients are erectile dysfunction (ED), painful ejaculation and premature ejaculation. So we will discuss about ED and ejaculation problems in CP/CPPS patients.

  8. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... its name, address, and principal business. (3) A current, dated, and signed financial statement... available. (6) Estimated cost and amount of loan needed. (7) Explanation of applicant's financial... under Public Law 103-354 can appropriately finance. (iii) There is an immediate and ready market for...

  9. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE... Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, CN 02150, Trenton, NJ 08650... Tennessee: Southeast Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, 77...

  10. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE... Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, CN 02150, Trenton, NJ 08650... Tennessee: Southeast Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, 77...

  11. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE... Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, CN 02150, Trenton, NJ 08650... Tennessee: Southeast Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, 77...

  12. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE... Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, CN 02150, Trenton, NJ 08650... Tennessee: Southeast Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, 77...

  13. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE... Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, CN 02150, Trenton, NJ 08650... Tennessee: Southeast Regional Office, U.S. Department of Agriculture, Food and Nutrition Service, 77...

  14. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... direct assignment where the indirect costs are attributable to the carrier's operations at the eligible place; (ii) By comparing the carrier's systemwide indirect operating expenses to those submitted by the carrier for the eligible place; or (iii) By comparing the indirect operating expenses submitted by...

  15. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... direct assignment where the indirect costs are attributable to the carrier's operations at the eligible place; (ii) By comparing the carrier's systemwide indirect operating expenses to those submitted by the carrier for the eligible place; or (iii) By comparing the indirect operating expenses submitted by...

  16. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... direct assignment where the indirect costs are attributable to the carrier's operations at the eligible place; (ii) By comparing the carrier's systemwide indirect operating expenses to those submitted by the carrier for the eligible place; or (iii) By comparing the indirect operating expenses submitted by...

  17. 48 CFR 1352.271-88 - Guarantees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... prescribed in 48 CFR 1371.119, insert the following clause: Guarantees (APR 2010) (a) In the event any work... redelivery of the vessel, the guarantee period shall run from the date of completion of such item. (d) If...

  18. 48 CFR 247.271-1 - Policy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) Award contracts, or exercise option years, before November 1 of each year, if possible. (b) Areas of... daily capability— (i) Will at least equal the maximum authorized individual weight allowance...

  19. 48 CFR 247.271-1 - Policy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) Award contracts, or exercise option years, before November 1 of each year, if possible. (b) Areas of... daily capability— (i) Will at least equal the maximum authorized individual weight allowance...

  20. 48 CFR 247.271-1 - Policy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Award contracts, or exercise option years, before November 1 of each year, if possible. (b) Areas of... daily capability— (i) Will at least equal the maximum authorized individual weight allowance...

  1. 48 CFR 247.271-1 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) Award contracts, or exercise option years, before November 1 of each year, if possible. (b) Areas of... daily capability— (i) Will at least equal the maximum authorized individual weight allowance...

  2. 38 CFR 17.271 - Eligibility.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... section. (1) The spouse or child of a veteran who has been adjudicated by VA as having a permanent and total service-connected disability; (2) The surviving spouse or child of a veteran who died as a result... permanently and totally disabled from a service-connected condition(s); (3) The surviving spouse or child of...

  3. 38 CFR 17.271 - Eligibility.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... section. (1) The spouse or child of a veteran who has been adjudicated by VA as having a permanent and total service-connected disability; (2) The surviving spouse or child of a veteran who died as a result... permanently and totally disabled from a service-connected condition(s); (3) The surviving spouse or child of...

  4. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... definitions, abbreviations, and methods of analysis set forth in 40 CFR part 401 shall apply to this subpart. (b) The term rare earth metals refers to the elements scandium, yttrium, and lanthanum to lutetium... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth...

  5. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as... analysis or organoleptic examination. (c) Transfers between brewery and pilot brewing plant. Subject...

  6. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as... analysis or organoleptic examination. (c) Transfers between brewery and pilot brewing plant. Subject...

  7. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as... analysis or organoleptic examination. (c) Transfers between brewery and pilot brewing plant. Subject...

  8. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as... analysis or organoleptic examination. (c) Transfers between brewery and pilot brewing plant. Subject...

  9. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as... analysis or organoleptic examination. (c) Transfers between brewery and pilot brewing plant. Subject...

  10. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CFR 2.23; delegation of authority by the Asst. Sec. for Rural Development, 7 CFR 2.70) ...-COOPERATIVE SERVICE, RURAL UTILITIES SERVICE, AND FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE LOANS AND... Obligation-Fund Analysis 2 2 1 1 1 1 Data input to Finance Office through field office terminals....

  11. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF... program of scholarly research. (f) Federal Reserve Bank means one of the district Banks authorized by the... this section) and which is operated solely for the purpose of conducting scientific research,...

  12. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CFR 2.23; delegation of authority by the Asst. Sec. for Rural Development, 7 CFR 2.70) ... individual sites planned together with a detailed plot plan. (iii) Preliminary engineering plans, if... docket must include: (1) A plot plan and detailed preliminary plans and specifications for development...

  13. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CFR 2.23; delegation of authority by the Asst. Sec. for Rural Development, 7 CFR 2.70) ... individual sites planned together with a detailed plot plan. (iii) Preliminary engineering plans, if... docket must include: (1) A plot plan and detailed preliminary plans and specifications for development...

  14. 7 CFR 1822.271 - Processing applications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CFR 2.23; delegation of authority by the Asst. Sec. for Rural Development, 7 CFR 2.70) ... individual sites planned together with a detailed plot plan. (iii) Preliminary engineering plans, if... docket must include: (1) A plot plan and detailed preliminary plans and specifications for development...

  15. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... used under title XVI of the Social Security Act (as set forth at 20 CFR part 416, subpart I... the List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and... regardless of their actual composition. In order to develop maximum SNAP allotments, the Secretary shall...

  16. 12 CFR 271.6 - Processing requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING... Office. The statement, certified to be true and correct to the best of the requester's knowledge and... Information Office or makes other acceptable arrangements, or the Committee deems it appropriate to send...

  17. High-Throughput Analytical Techniques for Determination of Residues of 653 Multiclass Pesticides and Chemical Pollutants in Tea, Part VI: Study of the Degradation of 271 Pesticide Residues in Aged Oolong Tea by Gas Chromatography-Tandem Mass Spectrometry and Its Application in Predicting the Residue Concentrations of Target Pesticides.

    PubMed

    Chang, Qiao-Ying; Pang, Guo-Fang; Fan, Chun-Lin; Chen, Hui; Wang, Zhi-Bin

    2016-07-01

    The degradation rate of 271 pesticide residues in aged Oolong tea at two spray concentrations, named a and b (a < b), were monitored for 120 days using GC-tandem MS (GC-MS/MS). To research the degradation trends and establish regression equations, determination days were plotted as horizontal ordinates and the residue concentrations of pesticide were plotted as vertical ordinates. Here, we consider the degradation equations of 271 pesticides over 40 and 120 days, summarize the degradation rates in six aspects (A-F), and discuss the degradation trends of the 271 pesticides in aged Oolong tea in detail. The results indicate that >70% of the determined pesticides coincide with the degradation regularity of trends A, B, and E, i.e., the concentration of pesticide will decrease within 4 months. Next, 20 representative pesticides were selected for further study at higher spray concentrations, named c and d (d > c > b > a), in aged Oolong tea over another 90 days. The determination days were plotted on the x-axis, and the differences between each determined result and first-time-determined value of target pesticides were plotted on the y-axis. The logarithmic function was obtained by fitting the 90-day determination results, allowing the degradation value of a target pesticide on a specific day to be calculated. These logarithmic functions at d concentration were applied to predict the residue concentrations of pesticides at c concentration. Results revealed that 70% of the 20 pesticides had the lower deviation ratios of predicted and measured results. PMID:27151741

  18. 40 CFR 97.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS... system under § 97.270(a)(1) (including the automated data acquisition and handling system) successfully... used under the CAIR SO2 Trading Program for a period not to exceed 120 days after receipt by...

  19. 40 CFR 96.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS FOR... continuous monitoring system under § 96.270(a)(1) (including the automated data acquisition and handling... provisionally certified monitoring system may be used under the CAIR SO2 Trading Program for a period not...

  20. 40 CFR 97.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS... system under § 97.270(a)(1) (including the automated data acquisition and handling system) successfully... used under the CAIR SO2 Trading Program for a period not to exceed 120 days after receipt by...

  1. 40 CFR 96.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS FOR... continuous monitoring system under § 96.270(a)(1) (including the automated data acquisition and handling... provisionally certified monitoring system may be used under the CAIR SO2 Trading Program for a period not...

  2. 40 CFR 97.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS... system under § 97.270(a)(1) (including the automated data acquisition and handling system) successfully... used under the CAIR SO2 Trading Program for a period not to exceed 120 days after receipt by...

  3. 40 CFR 96.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS FOR... continuous monitoring system under § 96.270(a)(1) (including the automated data acquisition and handling... provisionally certified monitoring system may be used under the CAIR SO2 Trading Program for a period not...

  4. 40 CFR 96.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO 2 TRADING PROGRAMS FOR... continuous monitoring system under § 96.270(a)(1) (including the automated data acquisition and handling... provisionally certified monitoring system may be used under the CAIR SO2 Trading Program for a period not...

  5. 40 CFR 97.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS... system under § 97.270(a)(1) (including the automated data acquisition and handling system) successfully... used under the CAIR SO2 Trading Program for a period not to exceed 120 days after receipt by...

  6. 40 CFR 96.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS FOR... continuous monitoring system under § 96.270(a)(1) (including the automated data acquisition and handling... provisionally certified monitoring system may be used under the CAIR SO2 Trading Program for a period not...

  7. 40 CFR 97.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS... system under § 97.270(a)(1) (including the automated data acquisition and handling system) successfully... used under the CAIR SO2 Trading Program for a period not to exceed 120 days after receipt by...

  8. 40 CFR 271.14 - Requirements for permitting.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...—(Permit by rule); (r) Section 270.61—(Emergency permits); (s) Section 270.64—(Interim permits for UIC....11—(Public comments and requests for hearings); (z) Section 124.12(a)—(Public hearings); and...

  9. 40 CFR 271.14 - Requirements for permitting.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...—(Permit by rule); (r) Section 270.61—(Emergency permits); (s) Section 270.64—(Interim permits for UIC....11—(Public comments and requests for hearings); (z) Section 124.12(a)—(Public hearings); and...

  10. 40 CFR 271.14 - Requirements for permitting.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...—(Permit by rule); (r) Section 270.61—(Emergency permits); (s) Section 270.64—(Interim permits for UIC....11—(Public comments and requests for hearings); (z) Section 124.12(a)—(Public hearings); and...

  11. 40 CFR 271.14 - Requirements for permitting.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...—(Permit by rule); (r) Section 270.61—(Emergency permits); (s) Section 270.64—(Interim permits for UIC....11—(Public comments and requests for hearings); (z) Section 124.12(a)—(Public hearings); and...

  12. 40 CFR 271.14 - Requirements for permitting.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...—(Permit by rule); (r) Section 270.61—(Emergency permits); (s) Section 270.64—(Interim permits for UIC....11—(Public comments and requests for hearings); (z) Section 124.12(a)—(Public hearings); and...

  13. 12 CFR 271.9 - Fee schedules; waiver of fees.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., duplication, and review apply when records are requested for commercial use. (2) Educational, research, or... requester is a representative of the news media or an educational or noncommercial scientific institution, whose purpose is scholarly or scientific research. The first 100 pages of duplication, however, will...

  14. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... waste program in violation of standards or regulations for management of such used oil; or who makes any... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility;...

  15. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... waste program in violation of standards or regulations for management of such used oil; or who makes any... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility;...

  16. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... waste program in violation of standards or regulations for management of such used oil; or who makes any... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility;...

  17. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... waste program in violation of standards or regulations for management of such used oil; or who makes any... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility;...

  18. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... waste program in violation of standards or regulations for management of such used oil; or who makes any... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility;...

  19. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Management of environmental factors, such as temperature, light, humidity, atmosphere, and air circulation... controls including but not limited to traps, light, or sound; or (2) Lures and repellents...

  20. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... used oil management which are equivalent to 40 CFR part 279. These standards shall include: (a... 29, in 40 CFR part 266, subpart E of this chapter. The part 279 of this chapter requirements... enforceable in those states that have not adopted state requirements equivalent to 40 CFR part 279, subparts...

  1. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is...

  2. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is...

  3. 47 CFR 25.271 - Control of transmitting stations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... station. (b) The licensee of a transmitting earth station licensed under this part shall ensure that a trained operator is present on the earth station site, or at a designated remote control point for the earth station, at all times that transmissions are being conducted. No operator's license is...

  4. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under the... states the conditions under which spectrum is made available and licensed for the provision of...

  5. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under the... states the conditions under which spectrum is made available and licensed for the provision of...

  6. 38 CFR 3.271 - Computation of income.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... income from a business, farm or profession as reduced by the necessary operating expenses such as cost of goods sold, or expenditures for rent, taxes, and upkeep, or costs of repairs or replacements. The value... other evidence of title shall constitute evidence of ownership. This includes property acquired...

  7. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... facilities to be owned by a Federal department or agency. You may use a PLA in leasehold arrangement... than $5 million. Project Labor Agreement (PLA) means an agreement between the contractor, subcontractors, and the union(s) representing workers. Under a PLA, the contractor and subcontractors on...

  8. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... facilities to be owned by a Federal department or agency. You may use a PLA in leasehold arrangement... than $5 million. Project Labor Agreement (PLA) means an agreement between the contractor, subcontractors, and the union(s) representing workers. Under a PLA, the contractor and subcontractors on...

  9. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... facilities to be owned by a Federal department or agency. You may use a PLA in leasehold arrangement... than $5 million. Project Labor Agreement (PLA) means an agreement between the contractor, subcontractors, and the union(s) representing workers. Under a PLA, the contractor and subcontractors on...

  10. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... facilities to be owned by a Federal department or agency. You may use a PLA in leasehold arrangement... than $5 million. Project Labor Agreement (PLA) means an agreement between the contractor, subcontractors, and the union(s) representing workers. Under a PLA, the contractor and subcontractors on...

  11. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... services. As prescribed in 48 CFR 1371.120, insert the following clause: Temporary Services (APR 2010) (a) Temporary services are services incidental to the performance of work which are required in the schedule or... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Temporary services....

  12. 12 CFR 271.9 - Fee schedules; waiver of fees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... section. These fees cover only the full allowable direct costs of search, duplication, and review. No fees... additional clarification before categorizing the request. (1) Commercial use. The fees for search... provided free. (3) All other uses. For all other requests, the fees for document search and...

  13. 12 CFR 271.9 - Fee schedules; waiver of fees.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... section. These fees cover only the full allowable direct costs of search, duplication, and review. No fees... additional clarification before categorizing the request. (1) Commercial use. The fees for search... provided free. (3) All other uses. For all other requests, the fees for document search and...

  14. 12 CFR 271.9 - Fee schedules; waiver of fees.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... section. These fees cover only the full allowable direct costs of search, duplication, and review. No fees... additional clarification before categorizing the request. (1) Commercial use. The fees for search... provided free. (3) All other uses. For all other requests, the fees for document search and...

  15. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Liability and insurance. As prescribed in 48 CFR 1371.110, insert the following clause: Liability and Insurance (APR 2010) (a) The contractor shall exercise reasonable care and use its best efforts to prevent... loss or damage the contractor by exercise of reasonable care was unable to prevent. However,...

  16. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of work. As prescribed in 48 CFR 1371.104, insert the following clause. Schedule of Work (APR 2010... shall identify the critical path. The Work Package Network shall show the work items, milestones, key events, and activities and shall clearly identify the critical path. The Total Manpower Loading...

  17. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... approved under 40 CFR 52.223 (37 FR 10842). (3) Calaveras County APCD. (i) Rules 110 and 402(f), submitted...) Fresno County APCD. (i) Rule 110, submitted on June 30, 1972, and previously approved under 40 CFR 52.223... approved under 40 CFR 52.223. (13) Los Angeles County APCD. (i) Rule 430, submitted on June 6, 1977....

  18. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... approved under 40 CFR 52.223 (37 FR 10842). (3) Calaveras County APCD. (i) Rules 110 and 402(f), submitted...) Fresno County APCD. (i) Rule 110, submitted on June 30, 1972, and previously approved under 40 CFR 52.223... approved under 40 CFR 52.223. (13) Los Angeles County APCD. (i) Rule 430, submitted on June 6, 1977....

  19. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... approved under 40 CFR 52.223 (37 FR 10842). (3) Calaveras County APCD. (i) Rules 110 and 402(f), submitted...) Fresno County APCD. (i) Rule 110, submitted on June 30, 1972, and previously approved under 40 CFR 52.223... approved under 40 CFR 52.223. (13) Los Angeles County APCD. (i) Rule 430, submitted on June 6, 1977....

  20. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... approved under 40 CFR 52.223 (37 FR 10842). (3) Calaveras County APCD. (i) Rules 110 and 402(f), submitted...) Fresno County APCD. (i) Rule 110, submitted on June 30, 1972, and previously approved under 40 CFR 52.223... approved under 40 CFR 52.223. (13) Los Angeles County APCD. (i) Rule 430, submitted on June 6, 1977....