Sample records for day culture period

  1. Interplanetary magnetic field periodicity of ˜153 days

    NASA Astrophysics Data System (ADS)

    Cane, H. V.; Richardson, I. G.; von Rosenvinge, T. T.

    We report on the finding of a 153-day periodicity in the magnetic field strength and solar wind speed measured at 1 AU during the years 1978-1982. The period and the occurence epoch are consistent with the “154-day” periodicity previously reported for events occurring at the Sun. In particular, the variations in the field strength and the occurence rate of energetic (tens of MeV) solar particle events are in phase. The similar periodicities in the interplanetary field and solar phenomena are consistent with a global phenomenon. Whereas this periodicity is quite strong for the magnetic field magnitude, there is only a weak periodicity for the individual field components. The field magnitude shows essentially no periodicity at this period during the previous and following solar maxima. The lack of persistence, and of significant harmonic components of the observed periodicity, does not support the proposal of a solar “clock” mechanism. The most significant variations in the complete near-earth magnetic field data base (1963-1997) with periods of less than 200 days occur at 166 and 146 days.

  2. Comparison of one-day versus two-day incubation of urine cultures

    Microsoft Academic Search

    Kim L. Joho; Hesham Soliman; Melvin P. Weinstein

    1995-01-01

    The value of incubating urine cultures for 1 versus 2 days was evaluated prospectively for 1526 consecutive specimens. A total of 507 cultures (33.2%) were positive after 1 day; 41 (2.7%) showed different results after 2 days. Only yeasts and corynebacteria were detected more often with longer incubation. Patient charts were available for review from 27 of 41 late positives;

  3. 150 Day Periodicities in the Voyager 2 Plasma Data

    NASA Astrophysics Data System (ADS)

    Ashmall, J.; Richardson, J. D.

    2003-12-01

    Launched over 26 years ago, Voyager 2 is now at a radial distance greater than 71 AU and a latitude of -28.7 degrees out of the ecliptic plane. Voyager 2 and the MIT Plasma Spectrometer continue to return measurements daily. Periodicities with T ? 150 days have been observed in the solar wind by several authors. Fourier and Lomb-Scargle periodograms of the uniquely long Voyager 2 plasma density and bulk-speed data sets (1978-2003) similarly show a strong component at periods of approximately 150 days. Dividing the data into two (1978.0-1990.5 and 1990.5-2003.0) and taking periodograms of the subsets shows a strong ~150 day component in the earlier data but not the latter. Periodograms of further sub-divisions of the data demonstrate a definite time-variation in the spectral power at periods around 150 days. To give a more quantitative view of time-variation of the periodicities, wavelet-analysis was employed. Initial results showed little or no power in the wavelet power spectra around 150 days. By using unusually (temporally) large wavelets, we demonstrate the existence of episodes of persistent but low-power periodicities in the Voyager plasma data, not detected by the smaller scale wavelets. Using large-scale wavelets reveals two episodes of power at ? 150 day periods (both several years long) in the density data and one episode in the bulk-speed data. We compare the bulk-speed and density results and discuss the relationship of the episodes to spacecraft position (radial distance and latitude) as well as to the solar cycle. Results are also compared to measurements of ~150 day periodicities by other authors. Voyager observations are sponsored, in part, by NASA/JPL.

  4. A 50 day periodicity in the cosmic ray anisotropy

    NASA Technical Reports Server (NTRS)

    Fillius, W.; Ip, W.-H.; Axford, I.

    1983-01-01

    A modulation of the anisotropy of relativistic interplanetary particles with a period of approximately 50 days is discussed. Even though it is not unusual for cosmic ray data to reflect the solar rotation frequency and its harmonics with periods of 25/n days (n being 1, 2, 3, . . .), it is not known how a periodicity of 50 days, which would be a subharmonic, is generated. Since the Cerenkov counter responds to all kinds of relativistic particles, it does not identify the particles that cause the 50 day east-west modulation. Jovian electrons are considered unlikely in that they are most abundant near their source, whereas the modulation has never been reported for ground-based neutron monitors. It is pointed out that since the Cerenkov threshold is somewhat lower than typical neutron monitor cutoffs, it is conceivable that the modulation affects nucleons just in this energy gap. It is also thought that there might be a uniformly distributed component of interplanetary electrons which undergo this peculiar modulation.

  5. 77 FR 64759 - Rescission of 10-Day Agency Discretionary Period in Assigning Unsatisfactory Safety Ratings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-23

    ...FMCSA-2012-0262] RIN 2126-AB55 Rescission of 10-Day Agency Discretionary Period in Assigning Unsatisfactory...provision indicating that the Agency will consider a 10- day extension of the 45-day period after which passenger and hazardous...

  6. Automating Partial Period Bond Valuation with Excel's Day Counting Functions

    ERIC Educational Resources Information Center

    Vicknair, David; Spruell, James

    2009-01-01

    An Excel model for calculating the actual price of bonds under a 30 day/month, 360 day/year day counting assumption by nesting the DAYS360 function within the PV function is developed. When programmed into an Excel spreadsheet, the model can accommodate annual and semiannual payment bonds sold on or between interest dates using six fundamental…

  7. Periodic CO2 Dosing Strategy for Dunaliella salina Batch Culture

    PubMed Central

    Ying, Kezhen; Gilmour, D. James; Zimmerman, William B.

    2015-01-01

    A periodic CO2 dosing strategy for D. salina 19/30 batch culture is proposed. A model of periodic CO2 dosing including dosing time calculation, dosing interval estimation and final chlorophyll yield prediction was established. In experiments, 5% CO2/95% N2 gas was periodically dosed into D. salina culture. Two different gas dosing flow rates were tested. The corresponding dosing time for each flow rate was estimated via the model (10 min·d?1 for 0.7 L·min?1 and 36 min·d?1 for 0.3 L·min?1). Daily pH measurements showed that the pH of these cultures dosed periodically was always kept between 7.5 and 9.5, which highlights that periodic gas supply can maintain a suitable range of pH for microalgal growth without expensive buffers. Notably the culture dosed for set daily intervals was seen to have similar growth to the culture supplied constantly, but with much higher CO2 capture efficiency (11%–18%) compared to continuous dosing (0.25%). It shows great potential for using periodic gas supply to reduce cost, wasted gas and energy use. PMID:25997005

  8. Periodic CO2 Dosing Strategy for Dunaliella salina Batch Culture.

    PubMed

    Ying, Kezhen; Gilmour, D James; Zimmerman, William B

    2015-01-01

    A periodic CO2 dosing strategy for D. salina 19/30 batch culture is proposed. A model of periodic CO2 dosing including dosing time calculation, dosing interval estimation and final chlorophyll yield prediction was established. In experiments, 5% CO2/95% N2 gas was periodically dosed into D. salina culture. Two different gas dosing flow rates were tested. The corresponding dosing time for each flow rate was estimated via the model (10 min·d-1 for 0.7 L·min-1 and 36 min·d-1 for 0.3 L·min-1). Daily pH measurements showed that the pH of these cultures dosed periodically was always kept between 7.5 and 9.5, which highlights that periodic gas supply can maintain a suitable range of pH for microalgal growth without expensive buffers. Notably the culture dosed for set daily intervals was seen to have similar growth to the culture supplied constantly, but with much higher CO2 capture efficiency (11%-18%) compared to continuous dosing (0.25%). It shows great potential for using periodic gas supply to reduce cost, wasted gas and energy use. PMID:25997005

  9. Teaching Culture in a North American Context: Mother's Day/Father's Day.

    ERIC Educational Resources Information Center

    Mollica, Anthony; Sterling, Tania

    1994-01-01

    Presents activities for teaching students about Mother's Day and Father's Day. Includes a fictional interview with Anna Jarvis, who helped start the Mother's Day holiday in the early 20th century. (MDM)

  10. 29 CFR 2590.715-2708 - Prohibition on waiting periods that exceed 90 days.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...becomes eligible for coverage on September 22, but for the waiting period...period for C would begin on September 22 and may not exceed 90 days...beginning on or after January 1, 2015. See § 2590.715-1251...of the orientation period is September 30. (f) * * *...

  11. Multicultural Day celebrates the cultural diversity of Notre Dame

    Microsoft Academic Search

    Michelle Ebbs

    2009-01-01

    Italian, Asian and Indian cuisine, Henna painting, Capoeira and a West African drumming performance were all part of celebrating this year’s Multicultural Day at The University of Notre Dame Australia’s Fremantle Campus.\\u000aMulticultural Day is celebrated annually by Notre Dame, with this year’s theme being Live. Eat. Love. Staff, students and community members were all invited to come together for

  12. Quasi-16-day periodic meridional movement of the equatorial ionization anomaly

    E-print Network

    Mo, X. H.

    Based on the daytime location of the equatorial ionization anomaly (EIA) crest derived from GPS observations at low latitude over China during the 2005–2006 stratospheric sudden warming (SSW), a quasi-16-day periodic ...

  13. 45 CFR 147.116 - Prohibition on waiting periods that exceed 90 days.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... becomes eligible for coverage on September 22, but for the waiting period...waiting period for C would begin on September 22 and may not exceed 90 days; therefore...beginning on or after January 1, 2015. See § 147.140 providing...

  14. Evolution of the 155 day periodicity in sunspot areas during solar cycles 12 to 21

    Microsoft Academic Search

    Judith Lean

    1990-01-01

    Sunspot area data during solar cycles 12-21 are examined with both periodogram and evolutionary spectral analysis techniques for evidence of a periodicity near 155 days. This periodicity is found to be present only during epochs of maxium activity, when it modulates the sunspot areas by as much as 15-20 percent of the amplitude of the 11 yr cycle. The 155

  15. Continuous Glucose Monitoring in the Subcutaneous Tissue over a 14-Day Sensor Wear Period

    PubMed Central

    Hoss, Udo; Budiman, Erwin S.; Liu, Hanqing; Christiansen, Mark P.

    2013-01-01

    Background Glucose monitoring systems using subcutaneously inserted sensors are currently labeled for up to 7 days of wear. In this study, we evaluated the feasibility of a 14-day wear duration using a modified version of the sensor found in the Freestyle Navigator™ continuous glucose monitoring system. Methods Sixty-two subjects with diabetes were enrolled in the study. One sensor per subject was inserted on the arm for a wear time of 14 days. Two different calibration algorithms were applied retrospectively, one that uses periodic sensor recalibrations and one without recalibrations. Sensor in vivo stability was determined by least square regression analysis using capillary blood glucose. Mean absolute relative difference (MARD) and mean relative difference were calculated. Consensus error grid analysis was performed by day and over the 14-day wear period to evaluate accuracy of both systems. The sensor insertion sites were inspected after sensor removal for skin reactions. Results Sensor data from 55 subjects were used for the analysis. The accuracy metrics for the system with recalibration were calculated to MARD = 13.9% and 84.0% in zone A (error grid analysis). The system without recalibration performed significantly better, resulting in MARD of 12.2% and 88.0% in zone A (p < .0001). The maximum change of in vivo sensor sensitivity over the 14-day wear period was 2% per day. Two subjects reported pain during the first 5 days of sensor wear, and 1 subject reported itching at the sensor site. No further skin reactions were noticed. Conclusions The study shows that a 14-day sensor wear period is achievable. Moreover, sensors using “wired enzyme” technology showed excellent in vivo stability, with no significant sensitivity loss over the 14-day wear period. PMID:24124948

  16. The 218 day period of the peculiar late B-type star HD 101584

    E-print Network

    Eric J. Bakker; Henny J. G. L. M. Lamers; L. B. F. M. Waters; Christoffel Waelkens

    1995-11-10

    We have searched for periodicity in the photometric and spectroscopic variations of the enigmatic star HD 101584 and found a long-term variability on a typical time scale of 1700 days (4.7 years) and a highly significant period of 218+-0.7 day. This period is most prominently present in the photometric indices which are a measure for the Balmer jump (Geneva d and Stromgren c). The Doppler velocities of the high-excitation photospheric absorption lines (HeI and CII) seem to be variable with the photometric period. Our data favor the 218 day period for the Doppler velocities with a small probability that the true period is 436 days. We argue that HD 101584 is a close (highly) eccentric 218 day binary system with a low-mass unseen secondary. The photometric and Doppler variations are attributed to changes in the velocity law and mass-loss rate of the stellar wind which lead to asymmetric line profiles and a phase dependent Balmer discontinuity. Binary interaction is responsible for the changes in velocity law and mass-loss rate leading to the observed phenomena.

  17. Detection of the 531-day-period wobble from the polar motion time series

    NASA Astrophysics Data System (ADS)

    Ding, H.; Shen, W.-B.

    2014-12-01

    After Cater (1981, 1982) weakly detected a 530-day-period wobble (531dW) in the polar motion, only few studies were addressed to the observations of this wobble. In this report, based the EOP C04 polar motion time series by using the ensemble empirical mode decomposition method, the 531dW of the polar motion was clearly observed. Here, we present main results, and the details can be found in Ding and Shen (2014). Key words: Polar motion time series; EEMD; 531-day-period wobble.

  18. Differential success in sampling of Atlantic Forest amphibians among different periods of the day.

    PubMed

    Rocha, Cfd; Siqueira, Cc; Ariani, Cv; Vrcibradic, D; Guedes, Dm; Kiefer, Mc; Almeida-Gomes, M; Goyannes-Araújo, P; Borges-Júnior, Vnt; Van Sluys, M

    2015-05-01

    In general, anurans tend to be nocturnal, though diurnal activity is characteristic of some groups. Studies show that frog activity may be inferred based on the number of individuals collected at different periods of the day, during large-scale field surveys. We investigated the best period of the day to conduct amphibian sampling in nine Atlantic Rainforest areas in southeastern Brazil, based on intensive field surveys. At each locality we employed similar sampling effort during diurnal, crepuscular and nocturnal searches (totaling 704.5 sampling hours). We pooled data from all localities for each period and estimated the proportion of frogs of each species active at each period based on the total number of individuals and on the number of species found during all surveys for that period. We recorded a total of 817 individual frogs from 69 species. Species richness was highest at night (median = 12 species), intermediate at dusk (median = 8), and lowest during the day (median = 4). The percentage of the total number of individual frogs found (pooled species) was highest during the night (ca. 53%) and lowest during the day (ca. 14%). Analyzing each species separately, the number of individuals recorded was consistently higher at dusk and night for most species. Our study evidences a trend for nocturnal activity for most Atlantic Rainforest frogs, with few species having primarily diurnal habits. Those results may favor future studies and conservation efforts for amphibian species. PMID:26132005

  19. Techniques to determine the quiet day curve for a long period of subionospheric VLF observations

    NASA Astrophysics Data System (ADS)

    Cresswell-Moorcock, Kathy; Rodger, Craig J.; Clilverd, Mark A.; Milling, David K.

    2015-05-01

    Very low frequency (VLF) transmissions propagating between the conducting Earth's surface and lower edge of the ionosphere have been used for decades to study the effect of space weather events on the upper atmosphere. The VLF response to these events can only be quantified by comparison of the observed signal to the estimated quiet time or undisturbed signal levels, known as the quiet day curve (QDC). A common QDC calculation approach for periods of investigation of up to several weeks is to use observations made on quiet days close to the days of interest. This approach is invalid when conditions are not quiet around the days of interest. Longer-term QDCs have also been created from specifically identified quiet days within the period and knowledge of propagation characteristics. This approach is time consuming and can be subjective. We present three algorithmic techniques, which are based on either (1) a mean of previous days' observations, (2) principal component analysis, or (3) the fast Fourier transform (FFT), to calculate the QDC for a long-period VLF data set without identification of specific quiet days as a basis. We demonstrate the effectiveness of the techniques at identifying the true QDCs of synthetic data sets created to mimic patterns seen in actual VLF data including responses to space weather events. We find that the most successful technique is to use a smoothing method, developed within the study, on the data set and then use the developed FFT algorithm. This technique is then applied to multiyear data sets of actual VLF observations.

  20. Regulation of period 1 expression in cultured rat pineal

    NASA Technical Reports Server (NTRS)

    Fukuhara, Chiaki; Dirden, James C.; Tosini, Gianluca

    2002-01-01

    The aim of the present study was to investigate the in vitro expression of Period 1 (Per1), Period 2 (Per2) and arylalkylamine N-acetyltransferase (AA-NAT) genes in the rat pineal gland to understand the mechanism(s) regulating the expression of these genes in this organ. Pineals, when maintained in vitro for 5 days, did not show circadian rhythmicity in the expression of any of the three genes monitored. Norepinephrine (NE) induced AA-NAT and Per1, whereas its effect on Per2 was negligible. Contrary to what was observed in other systems, NE stimulation did not induce circadian expression of Per1. The effect of NE on Per1 level was dose- and receptor subtype-dependent, and both cAMP and cGMP induced Per1. Per1 was not induced by repeated NE - or forskolin - stimulation. Protein synthesis was not necessary for NE-induced Per1, but it was for reduction of Per1 following NE stimulation. Per1 transcription in pinealocytes was activated by BMAL1/CLOCK. Our results indicate that important differences are present in the regulation of these genes in the mammalian pineal. Copyright 2002 S. Karger AG, Basel.

  1. A Simple Method for Multi-Day Imaging of Slice Cultures

    PubMed Central

    Seidl, Armin H.; Rubel, Edwin W

    2009-01-01

    The organotypic slice culture (Stoppini et al., 1991) has become the method of choice to answer a variety of questions in neuroscience. For many experiments however, it would be beneficial to image or manipulate a slice culture repeatedly, for example over the course of many days. We prepared organotypic slice cultures of the auditory brainstem of P3 and P4 mice and kept them in vitro for up to 4 weeks. Single cells in the auditory brainstem were transfected with plasmids expressing fluorescent proteins by way of electroporation (Haas et al., 2001). The culture was then placed in a chamber perfused with oxygenated ACSF and the labeled cell imaged with an inverted wide-field microscope repeatedly for multiple days, recording several time-points per day, before returning the slice to the incubator. We describe a simple method to image a slice culture preparation over to the course of multiple days and over many continuous hours, without noticeable damage to the tissue or photobleaching. Our method employs a simple, inexpensive custom-built insulator constructed around the microscope to maintain controlled temperature, and uses a perfusion chamber as used for in vitro slice recordings. PMID:19565635

  2. Eastward propagating planetary waves with periods of 15 days in the winter Antarctic stratosphere

    E-print Network

    Chu, Xinzhao

    as revealed by MERRA and lidar Xian Lu,1 Xinzhao Chu,1,2 Tim Fuller-Rowell,1,3 Loren Chang,4 Weichun Fong,1 are captured in the Modern Era Retrospective-Analysis for Research and Applications (MERRA) data and correspond to various phases of eastward propagating PWs with periods of 1­5 days as revealed in MERRA. The strongest PW

  3. A Possible 2.08 Day X-ray Period in Seyfert Galaxy Ton S180

    NASA Astrophysics Data System (ADS)

    Halpern, J. P.; Leighly, K. M.; Marshall, H. L.

    2002-12-01

    The Deep Survey instrument on the Extreme Ultraviolet Explorer satellite (EUVE) obtained a 33 day long, nearly continuous soft X-ray light curve of the Seyfert galaxy Ton S180 that is well suited to a search for transient periodicity or QPO in the range of hours to days that might be expected from dynamical processes in the inner accretion disk around a ~ 108 Msun black hole. Periodogram analysis of this highly variable light curve shows a feature at 2.08 days. We estimate the statistical significance of the signal using a method that carefully takes into account the red-noise properties of Seyfert light curves. The result is that the signal is significant at the 98% confidence level with respect to power-law noise. We also performed a uniform reduction of all of the EUVE light curves of 14 Seyfert galaxies and QSOs that exceed 5 days in duration, which account for a total of 231 days of observation. From the two observations (in addition to Ton S180) that were longer than 20 days in duration, we see a possible 0.89 day period in RX J0437.4--4711 with 96% significance, and a less significant signal at 5.8 days in 1H 0419--577. These period values appear unrelated to the length of the observation, which is similar in the three cases, but they do scale roughly as the luminosity of the object, which would be expected in dynamical scenarios such as orbital motion or trapped oscillations in the inner accretion disk, if luminosity scales with black-hole mass. Although it is generally true that variability amplitude in AGNs increases with increasing photon energy, it is now clear that EUV variability is as dramatic as any detected at higher energies. In many objects this component contains the bulk of the bolometric luminosity. Theory is in fact quite challenged to produce the large-amplitude EUV variability that we observe. Because of the energetics, one cannot beg the question of the mechanism of variability, as is often done, by appealing to reprocessing of harder X-rays. The EUV is where we must search for the fundamental reason why AGNs vary.

  4. A Possible 2.08 Day X-ray Period in Seyfert Galaxy Ton S180

    NASA Astrophysics Data System (ADS)

    Marshall, H. L.; Halpern, J. P.; Leighly, K. M.

    2003-03-01

    The Deep Survey instrument on the Extreme Ultraviolet Explorer satellite (EUVE) obtained a 33 day long, nearly continuous soft X-ray light curve of the Seyfert galaxy Ton S180 that is well suited to a search for transient periodicity or QPO in the range of hours to days that might be expected from dynamical processes in the inner accretion disk around a ˜ 108 Msun black hole. Periodogram analysis of this highly variable light curve shows a feature at 2.08 days. We estimate the statistical significance of the signal using a method that carefully takes into account the red-noise properties of Seyfert light curves. The result is that the signal is significant at the 98% confidence level with respect to power-law noise. We also performed a uniform reduction of all of the EUVE light curves of 14 Seyfert galaxies and QSOs that exceed 5 days in duration, which account for a total of 231 days of observation. From the two observations (in addition to Ton S180) that were longer than 20 days in duration, we see a possible 0.89 day period in RX J0437.4--4711 with 96% significance, and a less significant signal at 5.8 days in 1H 0419--577. These period values appear unrelated to the length of the observation, which is similar in the three cases, but they do scale roughly as the luminosity of the object, which would be expected in dynamical scenarios such as orbital motion or trapped oscillations in the inner accretion disk, if luminosity scales with black-hole mass. Although it is generally true that variability amplitude in AGNs increases with increasing photon energy, it is now clear that EUV variability is as dramatic as any detected at higher energies. In many objects this component contains the bulk of the bolometric luminosity. Theory is in fact quite challenged to produce the large-amplitude EUV variability that we observe. Because of the energetics, one cannot beg the question of the mechanism of variability, as is often done, by appealing to reprocessing of harder X-rays. The EUV is where we must search for the fundamental reason why AGNs vary.

  5. Russian Reading in a Period of Social and Cultural Change.

    ERIC Educational Resources Information Center

    Stelmakh, V. D.

    1995-01-01

    Addresses social and cultural changes of reading in Russia: (1) changes in cultural hierarchy; (2) changes in reader behavior; (3) declining importance of classic literature; and (4) rise of mass culture and its effects. Tables present information on the genre of books that were best sellers and books borrowed from Russian libraries. (JMV)

  6. Quasi-16-day period oscillations observed in middle atmospheric ozone and temperature in Antarctica

    NASA Astrophysics Data System (ADS)

    Demissie, T. D.; Kleinknecht, N. H.; Hibbins, R. E.; Espy, P. J.; Straub, C.

    2013-07-01

    Nightly averaged mesospheric temperature derived from the hydroxyl nightglow at Rothera station (67°34' S, 68°08' W) and nightly midnight measurements of ozone mixing ratio obtained from Troll station (72°01' S, 2°32' E) in Antarctica have been used to investigate the presence and vertical profile of the quasi-16-day planetary wave in the stratosphere and mesosphere during the Antarctic winter of 2009. The variations caused by planetary waves on the ozone mixing ratio and temperature are discussed, and spectral and cross-correlation analyses are performed to extract the wave amplitudes and to examine the vertical structure of the wave from 34 to 80 km. The results show that while planetary-wave signatures with periods 3-12 days are strong below the stratopause, the oscillations associated with the 16-day wave are the strongest and present in both the mesosphere and stratosphere. The period of the wave is found to increase below 42 km due to the Doppler shifting by the strong eastward zonal wind. The 16-day oscillation in the temperature is found to be correlated and phase coherent with the corresponding oscillation observed in O3 volume mixing ratio at all levels, and the wave is found to have vertical phase fronts consistent with a normal mode structure.

  7. Geopathology on May Day: Expressions of Culture on Hawai'i's Elementary School Stages

    ERIC Educational Resources Information Center

    Steele, Jamie Simpson

    2013-01-01

    In Hawai'i's elementary schools, May Day programs feature children adorned with flower leis, singing and dancing hula about Hawaiian culture and performing traditions from major ethnic groups who settled the islands. Using the lens of geopathology, this research questions how various groups of residents long for belonging and struggle for…

  8. The "missing link": a 4-day period transiting exoplanet around OGLE-TR-111

    E-print Network

    Frederic Pont; Francois Bouchy; Didier Queloz; Nuno Santos; Claudio Melo; Michel Mayor; Stephane Udry

    2004-08-26

    We report the discovery of a transiting hot Jupiter around OGLE-TR-111, from our radial velocity follow-up of OGLE transiting candidates in Carina. The planet has a mass of 0.53 +- 0.11 M_J and a radius of 1.0 +0.13-0.06 R_J. Three transiting exoplanets have already been found among OGLE candidates, all with periods near 1.5 days. The planet presented here, with P=4.0 days, is the first exoplanet detected by transits with the characteristics of a "normal" hot Jupiter, as found in abundance by radial velocity surveys The radius of OGLE-TR-111b and the scarcity of hot Jupiters detected among OGLE transit candidates tend to indicate that the case of HD209458b, with a radius of 1.4 R_J, is exceptional, with most hot Jupiters being smaller.

  9. WASP-117b: a 10-day-period Saturn in an eccentric and misaligned orbit

    NASA Astrophysics Data System (ADS)

    Lendl, M.; Triaud, A. H. M. J.; Anderson, D. R.; Collier Cameron, A.; Delrez, L.; Doyle, A. P.; Gillon, M.; Hellier, C.; Jehin, E.; Maxted, P. F. L.; Neveu-VanMalle, M.; Pepe, F.; Pollacco, D.; Queloz, D.; Ségransan, D.; Smalley, B.; Smith, A. M. S.; Udry, S.; Van Grootel, V.; West, R. G.

    2014-08-01

    We report the discovery of WASP-117b, the first planet with a period beyond 10 days found by the WASP survey. The planet has a mass of Mp = 0.2755 ± 0.0089 MJ, a radius of Rp= 1.021_{-0.065+0.076 Rjup} and is in an eccentric (e = 0.302 ± 0.023), 10.02165 ± 0.00055 d orbit around a main-sequence F9 star. The host star's brightness (V = 10.15 mag) makes WASP-117 a good target for follow-up observations, and with a periastron planetary equilibrium temperature of Teq= 1225_{-39+36} K and a low planetary mean density (?p= 0.259_{-0.048+0.054 ?jup}) it is one of the best targets for transmission spectroscopy among planets with periods around 10 days. From a measurement of the Rossiter-McLaughlin effect, we infer a projected angle between the planetary orbit and stellar spin axes of ? = -44 ± 11 deg, and we further derive an orbital obliquity of ? = 69.6 +4.7-4.1 deg. Owing to the large orbital separation, tidal forces causing orbital circularization and realignment of the planetary orbit with the stellar plane are weak, having had little impact on the planetary orbit over the system lifetime. WASP-117b joins a small sample of transiting giant planets with well characterized orbits at periods above 8 days. Based on data obtained with WASP-South, CORALIE and EulerCam at the Euler-Swiss telescope, TRAPPIST, and HARPS at the ESO 3.6 m telescope (Prog. IDs 087.C-0649, 089.C-0151, 090.C-0540)Photometric and radial velocities are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/568/A81

  10. On the nonstationarity of the decadal periodicities of the length of day

    NASA Astrophysics Data System (ADS)

    De Michelis, P.; Tozzi, R.; Consolini, G.

    2013-12-01

    The Earth's rotation rate is not constant, but changes on all observable timescales, from subdaily to decadal and longer. These variations are usually discussed in terms of variations in the length of the day (LoD) and are caused by processes acting within the interior, at the surface and outside of the Earth. Here, we investigate the presence of long-standing decadal variations in yearly LoD data covering the period from 1832 to 2009 by applying the Hilbert-Huang transform (HHT). The HHT has been slightly modified here to take into account the uncertainty of LoD values that has changed greatly in time due to the use of different LoD measurement techniques. The LoD time series has been completely decomposed into five intrinsic mode functions (IMF) and a residual trend. The estimation of instantaneous frequencies and related amplitudes of the obtained IMFs has allowed us to compute the Hilbert spectrum that has been used as the starting point for studying and discussing the stationarity of typical LoD timescale stationarity. The obtained results while showing the presence of multiple periodicities also indicate the absence of really stationary periodicities. Therefore, rather than considering the processes taking place in the Earth's core as the result of a superposition of oscillations (i.e. stationary mechanisms) occurring on a discrete number of different timescales, it would be better to think of a superposition of fluctuations that are intermittent in both frequency and amplitude.

  11. Comparison of four media types during 3-day human IVF embryo culture

    Microsoft Academic Search

    Vincent W Aoki; Aaron L Wilcox; C Matthew Peterson; Kirtly Parker-Jones; Harry H Hatasaka; Mark Gibson; Ivan Huang; Douglas T Carrell

    2005-01-01

    The aim of this study was to compare the effectiveness of human tubal fluid (HTF), G1.2®, Sage Cleavage® and Life Global® media for IVF outcome during 3-day culture of human embryos. A three-phase auto-controlled study was conducted in which IVF outcome was compared between (1) HTF and G1.2, (2) HTF and Cleavage, and (3) Cleavage and Life Global. In phase

  12. G328: A Small-Amplitude Red Variable with a Period Near One Day

    NASA Astrophysics Data System (ADS)

    Yao, Bao-An; Shen, Chang-Jun; Zhang, Chun-Sheng; Hu, Han-Ming; Lin, Qing

    2006-10-01

    G328 = A65 = L3314 (V = 13.83, B-V = 1.91) is a field star in the direction of the globular cluster M4. If we take E(B-V) = 0.40, then its (B-V)0 = 1.51, corresponding to a spectral type of K5III if it is a giant star; or of dM2 if it is a dwarf. Observations at both the MSSSO and Yunnan Observatory have shown that G328 is a new variable with peak to peak amplitude simeq 0.05 mag in V. While it is not unusual for so red a star to be a variable, special attention must be paid to its short period of about one day. If the variability is due to pulsation, the spectral type and luminosity as well as effective temperature should be determined in order to compare it with Xiong's theory.

  13. X 1908+075 An X-ray Binary with a 4.4 day Period

    E-print Network

    Wen, L; Bradt, H V; Wen, Linqing; Remillard, Ronald A.; Bradt, Hale V.

    1999-01-01

    X 1908+075 is an optically unidentified and highly absorbed X-ray source that appears in early surveys such as Uhuru, OSO-7, Ariel V, HEAO-1, and the EXOSAT Galactic Plane Survey. These surveys measured a source intensity in the range of 2-12 mCrab at 2-10 keV, and the position was localized to ~ 0.5 degrees. We use the Rossi X-ray Timing Explorer (RXTE) All Sky Monitor (ASM) to confirm our expectation that a particular Einstein IPC detection (1E 1908.4+0730) provides the correct position for X 1908+075. The analysis of the coded mask shadows from the ASM for the position of 1E 1908.4+0730 yields a persistent intensity ~ 8 mCrab (1.5-12 keV) over a 3 year interval beginning in 1996 February. Furthermore, we detect a period of 4.400 +- 0.001 days with a false alarm probability < 1.0e-7 . The folded light curve is roughly sinusoidal, with an amplitude that is 22 % of the mean flux. The X-ray period may be attributed to the scattering and absorption of X-rays through a stellar wind combined with the orbital m...

  14. X1908+075: An X-Ray Binary with a 4.4 Day Period

    NASA Astrophysics Data System (ADS)

    Wen, Linqing; Remillard, Ronald A.; Bradt, Hale V.

    2000-04-01

    X1908+075 is an optically unidentified and highly absorbed X-ray source that appeared in early surveys such as Uhuru, OSO 7, Ariel 5, HEAO-1, and the EXOSAT Galactic Plane Survey. These surveys measured a source intensity in the range 2-12 mcrab at 2-10 keV, and the position was localized to ~0.5d. We use the Rossi X-Ray Timing Explorer (RXTE) All-Sky Monitor (ASM) to confirm our expectation that a particular Einstein/IPC detection (1E 1908.4+0730) provides the correct position for X1908+075. The analysis of the coded mask shadows from the ASM for the position of 1E 1908.4+0730 yields a persistent intensity ~8 mcrab (1.5-12 keV) over a 3 yr interval beginning in 1996 February. Furthermore, we detect a period of 4.400+/-0.001 days with a false-alarm probability less than 10-7. The folded light curve is roughly sinusoidal, with an amplitude that is 26% of the mean flux. The X-ray period may be attributed to the scattering and absorption of X-rays through a stellar wind combined with the orbital motion in a binary system. We suggest that X1908+075 is an X-ray binary with a high-mass companion star.

  15. X 1908+075: An X-ray Binary with a 4.4 day Period

    E-print Network

    Linqing Wen; Ronald A. Remillard; Hale V. Bradt

    1999-07-29

    X 1908+075 is an optically unidentified and highly absorbed X-ray source that appears in early surveys such as Uhuru, OSO-7, Ariel V, HEAO-1, and the EXOSAT Galactic Plane Survey. These surveys measured a source intensity in the range of 2-12 mCrab at 2-10 keV, and the position was localized to ~ 0.5 degrees. We use the Rossi X-ray Timing Explorer (RXTE) All Sky Monitor (ASM) to confirm our expectation that a particular Einstein IPC detection (1E 1908.4+0730) provides the correct position for X 1908+075. The analysis of the coded mask shadows from the ASM for the position of 1E 1908.4+0730 yields a persistent intensity ~ 8 mCrab (1.5-12 keV) over a 3 year interval beginning in 1996 February. Furthermore, we detect a period of 4.400 +- 0.001 days with a false alarm probability < 1.0e-7 . The folded light curve is roughly sinusoidal, with an amplitude that is 22 % of the mean flux. The X-ray period may be attributed to the scattering and absorption of X-rays through a stellar wind combined with the orbital motion in a binary system. We suggest that X 1908+075 is an X-ray binary with a high mass companion star.

  16. Search for the 531 day-period wobble signal in the polar motion based on EEMD

    NASA Astrophysics Data System (ADS)

    Ding, H.; Shen, W. B.

    2015-04-01

    In this study, we use a nonlinear and non-stationary time series analysis method, the ensemble empirical mode decomposition method (EEMD), to analyze the polar motion (PM) time series (EOP C04 series from 1962 to 2013) to find a 531 day-period wobble (531 dW) signal. The 531 dW signal has been found in the early PM seires (1962-1977) while cannot be found in the recent PM seires (1978-2013) using conventional analysis approaches. By the virtue of the demodulation feature of EEMD, the 531 dW can be confirmed to be present in PM based on the differences of the amplitudes and phases between different intrinsic mode functions. Results from three sub-series divided from the EOP C04 series show that the period of the 531 dW is subject to variations, in the range of 530.9-524 d, and its amplitude is also time-dependent (about 2-11 mas). Synthetic tests are carried out to explain why the 531 dW can only be observed in recent 30-years PM time series after using EEMD. The 531 dW is also detected in two longest available superconducting gravimeter (SG) records, which further confirms the presence of the 531 dW. The confirmation of 531 dW existence could be significant in establishing a more reasonable Earth rotation model and may effectively contribute to the prediction of the PM and its mechanism interpretation.

  17. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... false Reduction of inpatient psychiatric benefit days available in the initial benefit period. 409.63 Section 409.63 Public...SERVICES MEDICARE PROGRAM HOSPITAL INSURANCE BENEFITS Scope of Hospital Insurance Benefits §...

  18. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... false Reduction of inpatient psychiatric benefit days available in the initial benefit period. 409.63 Section 409.63 Public...SERVICES MEDICARE PROGRAM HOSPITAL INSURANCE BENEFITS Scope of Hospital Insurance Benefits §...

  19. Mediterraneità and modernità : architecture and culture during the period of Italian colonization of North Africa

    E-print Network

    McLaren, Brian L. (Brian Lloyd), 1958-

    2001-01-01

    This dissertation examines the intersection of the modern and the colonial in architecture and culture during the period of Italian colonization of North Africa from 1911 to 1943. Rather than see the colonies as merely a ...

  20. A New Model for Long-Period Tidal Variations in Length of Day

    NASA Astrophysics Data System (ADS)

    Ray, R. D.; Erofeeva, S.

    2012-12-01

    The standard IERS model for tidal variations in length of day (LOD), covering the band from one week to 18.6 years, stems from a 30-year-old paper by Yoder, Williams, and Parke. Their original table has been updated in the latest adopted standards by modifying only a few constituents: four constituents were adjusted for effects of mantle anelasticity and two constituents for effects of ocean tides. We here present a new, consistent, and comprehensive model. While Yoder et al. employed 62 tidal spectral lines, we employ 80, most of the new lines being higher frequency lines that are now detectable, or nearly detectable, in LOD measurements. We employ an anelastic model of Wahr and Bergen consistently to all lines throughout the entire long-period band, although there remains uncertainty in extrapolating to the lowest frequencies. Our model of ocean tides builds on recent experience modeling the fortnightly Mf tide (Ray and Egbert, Geophys. J. Int., 2012), for which good observational data allow extensive testing of both the numerical ocean model and the Wahr-Bergen anelastic model. Ocean model improvements now appear adequate to remove our dependence on data assimilation for Mf (at least for earth-rotation effects), which is a critical requirement before extending to other frequencies that lack reliable observational constraints. We have run the model for 12 major long-period constituents. The ocean-tide admittance at these 12 frequencies is then interpolated smoothly to all other lines, while relaxing to self-consistent equilibrium for the node tide. Application of this new model to a modern time series of space-geodetic LOD measurements shows a significant reduction in variance compared with the current IERS model. For example, in the 10-70 cpy band, the variance is reduced by 8%.

  1. Auroral and thermospheric response to the 9 day periodic variations in the dayside reconnection rate in 2005

    Microsoft Academic Search

    Yongliang Zhang; Larry J. Paxton; Daniel Morrison

    2010-01-01

    In this paper we seek the means to forecast changes in thermospheric composition. In particular, we focus on a period where there was a strong 9 day periodicity in the high-latitude drivers, as this provides the best opportunity to explore our ability to determine the signature of the drivers that change the thermosphere. Using measurements from ACE and TIMED\\/GUVI in

  2. CTEPP NC DATA COLLECTED ON FORM 10 (PERIODS 1-3): DAY CARE CENTER CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data set contains data concerning the child?s activities at the day care center over the 48-h monitoring period. The diary was divided into three time periods over the 48-h monitoring interval. The Food Survey collected information on the frequency and types of fruits, veget...

  3. CTEPP DATA COLLECTION FORM 10 (PERIODS 1-3): DAY CARE CENTER CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form collects information on the child's activities at the day care center over the 48-hr monitoring period. The diary is divided into three time periods over the 48-monitoring interval. The Food Survey collects information on the frequency and types of frui...

  4. Voids and Question Marks in the Present-day Data Concerning the Rotation Period of the First 1000 Numbered Asteroids

    NASA Astrophysics Data System (ADS)

    Alvarez, Eduardo Manuel

    2015-04-01

    Currently, there are only 19 three-digit numbered asteroids - none of them within the first 500 - for which their rotation period is unknown. Chances are that all of the first 1000 asteroids will have a known period in just a few years. However, not all of the 981 present-day published rotation period data for asteroids numbered below 1000 are secure. Ongoing investigations to verify, refine, or revise existing periods remains an important endeavor, especially for the 17 asteroids for which the period is currently uncertain.

  5. Discovery of a 115 Day Orbital Period in the Ultraluminous X-ray Source NGC 5408 X-1

    NASA Technical Reports Server (NTRS)

    Strohmayer, Tod E.

    2009-01-01

    We report the detection of a 115 day periodicity in SWIFT/XRT monitoring data from the ultraluminous X-ray source (ULX) NGC 5408 X-1. Our o ngoing campaign samples its X-ray flux approximately twice weekly and has now achieved a temporal baseline of ti 485 days. Periodogram ana lysis reveals a significant periodicity with a period of 115.5 +/- 4 days. The modulation is detected with a significance of 3.2 x 10(exp -4) . The fractional modulation amplitude decreases with increasing e nergy, ranging from 0.13 +/- 0.02 above 1 keV to 0.24 +/- 0.02 below 1 keV. The shape of the profile evolves as well, becoming less sharply peaked at higher energies. The periodogram analysis is consistent wi th a periodic process, however, continued monitoring is required to c onfirm the coherent nature of the modulation. Spectral analysis indic ates that NGC 5408 X-1 can reach 0.3 - 10 keV luminosities of approxi mately 2 x 10 40 ergs/s . We suggest that, like the 62 day period of the ULX in M82 (X41.4-1-60), the periodicity detected in NGC 5408 X-1 represents the orbital period of the black hole binary containing the ULX. If this is true then the secondary can only be a giant or super giant star.

  6. Evidence for a 115 Day Orbital Period in the Ultraluminous X-ray Source NGC 5408 X-1

    NASA Technical Reports Server (NTRS)

    Strohmayer, T.

    2009-01-01

    We report the detection of a 115 day periodicity in SWIFT/XRT monitoring data from the ultraluminous X-ray source (ULX) NGC 5408 X-1. Our ongoing campaign samples its X-ray flux approximately twice weekly and has now achieved a temporal baseline of more than 500 days. Timing analysis reveals a significant periodicity with a period of 115.5 +- 4 days. The fractional modulation amplitude decreases with increasing energy, ranging from 0.13 above 1 keV to 0.24 below 1 keV. The shape of the profile evolves as well, becoming less sharply peaked at higher energies. Periodogram analysis is consistent with a periodic process, however, continued monitoring is required to confirm the coherent nature of the modulation. Spectral analysis indicates that NGC 5408 X-1 can reach 0.3 - 10 keV luminosities of 2 x 10e40 ergs/s. We suggest that, like the 62 day period of the ULX in M82 (X41.4+60), the periodicity detected in NGC 5408 X-1 represents the orbital period of the black hole binary containing the ULX. If this is true then the secondary can only be a giant or supergiant star.

  7. "A Day in the Life": Advancing a Methodology for the Cultural Study of Development and Learning in Early Childhood

    ERIC Educational Resources Information Center

    Gillen, Julia; Cameron, Catherine Ann; Tapanya, Sombat; Pinto, Giuliana; Hancock, Roger; Young, Susan; Gamannossi, Beatrice Accorti

    2007-01-01

    This paper explores the methodology of an ecological investigation of aspects of culture in the interactional construction of early childhood in diverse global communities: Peru, Italy, Canada, Thailand, and the United Kingdom. Regarding culture as a dynamic dimension of the child's socialisation, the approach taken was to film a "day in the life"…

  8. 78 FR 36560 - 30-Day Notice of Proposed Information Collection: FHA Lender Approval, Annual Renewal, Periodic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-18

    ...Information Collection: FHA Lender Approval, Annual Renewal, Periodic Updates and Noncompliance Reporting by FHA Approved Lenders...Information Collection: FHA Lender Approval, Annual Renewal, Periodic Updates and Noncompliance Reporting by FHA Approved...

  9. Coccidiosis control by administering toltrazuril in the drinking water for a 2-day period.

    PubMed

    Mathis, G F; Froyman, R; Kennedy, T

    2004-05-01

    A 56-day floor pen study was conducted to determine the appropriate time to administer toltrazuril (Baycox) (TOL) for control of coccidiosis in broiler chickens. Litter was seeded with field strains of Eimeria acervulina, Eimeria maxima and Eimeria tenella. On Days 0, 21, 35 and 56, all birds and feed were weighed. Starting on Day 14, weekly lesion scores and oocyst counts were performed. The treatments were 125 ppm nicarb (NIC) in the starter to 66 ppm salinomycin (SAL) in the grower with no TOL (NIC/SAL/no TOL), 66 ppm salinomycin in both the starter and the grower but no TOL (SAL/SAL/no TOL), or no in-feed medication with the following TOL treatment: TOL Days 2-3, TOL Days 6-7, TOL Days 10-11, TOL Days 14-15, TOL Days 18-19, and as control NM/NM/no TOL (NM). The withdrawal feed was nonmedicated. TOL was administered in the drinking water at the rate of 7 mg/kg body weight. Oocysts per gram litter and lesion scores showed a significant infection in the NM birds, which peaked about Day 21. The NIC/SAL gave excellent early protection but only moderate protection during the SAL phase. The final performance for the SAL/SAL was significantly less compared to all TOL and NIC/SAL birds. All TOL treatments but Days 2-3 provided good coccidiosis control with accompanying performance. The absence of clinical coccidiosis relapse during the last third of the growout along with moderate oocyst counts and low lesions was indicative of unimpaired coccidiosis immunity. It can be inferred from the overall results that the use of TOL as the sole anticoccidial for two consecutive days in the drinking water between Days 10 and 14 would be the best time for good coccidiosis control allowing full performance. PMID:15110398

  10. Variations of the magnetic fields of the sun and the earth in 7–50 day periods

    Microsoft Academic Search

    V. P. Bobova; N. N. Stepanian

    1994-01-01

    The time variations of solar and terrestrial magnetic fields (background magnetic field, power of the active regions, AE and aa-indices) have been studied. The analysis of these data shows that multiplets of 27, 13.5, 9 and 7 day periods exist in the solar data as in the terrestrial data. The solar multiplets 13.5 and 9 days appear predominantly close to

  11. The Evolution of Cloud Features on Neptune over a 20-day time period

    NASA Astrophysics Data System (ADS)

    Martin, S. C.; Roe, H.; de Pater, I.; Macintosh, B.; Gibbard, S.; Max, C. E.; Gavel, D.; Brown, M.; Ghez, A.

    2000-10-01

    Near-infrared images of Neptune were taken with SCAM, the slit-viewing camera of the AO/NIRSPEC system at the 10-m W.M. Keck II telescope, on 2000 June 8,17,18,22,23 and 29 UT. Observations with broad-band filter Nirspec-5 [1.61 micron], Nirspec-6 [1.93 micron], and K' [2.12 micron], and several narrow-band filters between 1.279 and 1.702 microns are compared to determine the temporal evolution of cloud features over the 20-day period. These images taken with the Keck Adaptive Optics system are spectacular, revealing narrow zonal bands at 3-4 degree latitude spacings within bright regions and near the equator. There are three regions of latitude that contain bright features along zonal bands. The dimmest of these bright regions is located in the Northern hemisphere and extends from 20 deg N to 40 deg N. The remaining two bright regions are located in the Southern hemisphere extending from 20 deg S to 50 deg S, and from 60 deg S to 70 deg S. The region near the South pole (a recently quiet location) contains a bright teardrop-shaped feature which may be related to the Westerly jet observed by Voyager and reported by Sanjay et.al. (Journal of Geophysical Research, vol.6, pp. 18941-18960, 1991) at a similar latitude. This research was supported in part by the STC Program of the National Science Foundation under Agreement No. AST-9876783, and in part under the auspices of the US Department of Energy at Lawrence Livermore National Laboratory, Univ. of Calif. under contract No. W-7405-Eng-48. Data presented herein were obtained at the W.M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W.M. Keck Foundation.

  12. 20 CFR 1002.196 - What is the employee's reemployment position if the period of service was less than 91 days?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...position if the period of service was less than 91 days? 1002.196 Section...position if the period of service was less than 91 days? Following a period of service in the uniformed services of less than 91 days, the employee must...

  13. Building enterprise-wide resilience by integrating business continuity capability into day-to-day business culture and technology.

    PubMed

    Alesi, Patrick

    2008-04-01

    This paper follows the development of the business continuity planning (BCP) programme at Lehman Brothers following the events of September 11th. Previous attempts to implement a `traditional' form of BCP had been ineffective, but following the events, the firm began to look at BCP in a new light. This paper deals with three main themes: creating a culture of resiliency, leveraging technology, and building flexible plans. Distributing accountability for BCP to business line managers, integrating BCP change management into the normal course of business, and providing every employee with personalised BCP information breeds a culture of resiliency where people are empowered to react to events without burdensome, hierarchical response and recovery procedures. Building a strong relationship with one's application development community can result in novel, customised BCP solutions; existing systems and data structures can be used to enhance an existing BCP. Even the best plans are often challenged by events; understanding that flexibility is essential to effective incident response is a critical element in the development of a proper business continuity plan. PMID:21339108

  14. Finding a 24 Day Orbital Period for the X-Ray Binary 1A 1118-616

    NASA Technical Reports Server (NTRS)

    Staubert, R.; Pottschmidt, K.; Doroshenko, V.; Wilms, J.; Suchy, S.; Rothschild, R.; Santangelo, A.

    2010-01-01

    We report the first determination of the binary period and the orbital ephemeris of the Be X-ray binary containing the pulsar IA 1118-616 (35 years after the discovery of the source). The orbital period is found to be P(sub orb) = 24.0+/-0.4 days. The source was observed by RXTE during its last big X-ray outburst in January 2009, peaking at MJD 54845.4. This outburst was sampled by taking short observations every few days, covering an elapsed time comparable to the orbital period. Using the phase connection technique, pulse arrival time delays could be measured and an orbital solution determined. The data are consistent with a circular orbit, the time of 90 degrees longitude was found to be T,/2 = MJD 54845.37(10), coincident with the peak X-ray flux.

  15. Comparison of SAFER behavior assessment results in shelter dogs at intake and after a 3-day acclimation period.

    PubMed

    Bennett, Sara L; Weng, Hsin-Yi; Walker, Sheryl L; Placer, Margaret; Litster, Annette

    2015-01-01

    In this study, it was hypothesized that different results would be obtained by canine behavior assessments performed within 24 hr of shelter intake (Day 0) and after a 3-day acclimation period (Day 3). Safety Assessment for Evaluating Rehoming assessments were performed on 33 dogs at 2 municipal shelters. Agreements between Day 0 and Day 3 varied among subtests, and no consistent temporal patterns were observed. Weighted kappa statistics for each subtest ranged from .28 to .78, and percentage discordance was 0% to 18%. In a 2nd analysis, subtests skipped due to serious aggression were replaced with scores corresponding to serious aggression, and missing values for the Food subtest were replaced with scores for no aggression if the dog did not eat. For subtests skipped due to severe aggression, more than 50% of the dogs had scores indicating low aggression on the other assessment. Eight of 16 dogs who did not eat on Day 0 ate on Day 3; 2 showed aggression. Until the ideal time to test can be identified, it should be based on the individual dog's welfare status, and testing of dogs showing severe stress should be avoided. PMID:25603466

  16. A degree-day model for the latent period of stagonospora nodorum blotch in winter wheat

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Stagonospora nodorum blotch (SNB), which is caused by Stagonospora nodorum, occurs frequently in the southeastern United States and severe epidemics can lead to substantial economic losses. To establish a model for the development of SNB based on the effects of temperature on the latent period of th...

  17. Physical Properties of the 0.94Day Period Transiting Planetary System WASP18

    Microsoft Academic Search

    John Southworth; T. C. Hinse; M. Dominik; M. Glitrup; U. G. Jørgensen; C. Liebig; M. Mathiasen; D. R. Anderson; V. Bozza; P. Browne; M. Burgdorf; S. Calchi Novati; S. Dreizler; F. Finet; K. Harpsøe; F. Hessman; M. Hundertmark; G. Maier; L. Mancini; P. F. L. Maxted; S. Rahvar; D. Ricci; G. Scarpetta; J. Skottfelt; C. Snodgrass; J. Surdej; F. Zimmer

    2009-01-01

    We present high-precision photometry of five consecutive transits of WASP-18, an extrasolar planetary system with one of the shortest orbital periods known. Through the use of telescope defocusing we achieve a photometric precision of 0.47-0.83 mmag per observation over complete transit events. The data are analyzed using the JKTEBOP code and three different sets of stellar evolutionary models. We find

  18. INTERPRETATION OF THE 115 DAY PERIODIC MODULATION IN THE X-RAY FLUX OF NGC 5408 X-1

    SciTech Connect

    Foster, D. L.; Charles, P. A. [South African Astronomical Observatory, P.O. Box 9, Observatory, 7935 (South Africa); Holley-Bockelmann, K., E-mail: deatrick@saao.ac.z [Department of Physics and Astronomy, Vanderbilt University, Nashville, TN (United States)

    2010-12-20

    We comment on the recent observation of a 115 day modulation in the X-ray flux of the ultraluminous X-ray source (ULX) NGC 5408 X-1, and in particular, the interpretation of this modulation as the orbital period. We suggest that this modulation may instead be due to a precessing jet, and is thus superorbital in nature. Comparing the properties of this ULX with those of the prototypical micro-quasar SS 433, we argue that NGC 5408 X-1 is very similar to SS 433: a hyper-accreting stellar-mass black hole in a shorter-period binary. If the analogy holds, the 115 day modulation is best explained by the still poorly understood physics of inner-disk/jet precession and a longer observing baseline would be able to reveal an intrinsic phase jitter that is associated with such a precession.

  19. The role of 3D microenvironmental organization in MCF-7 epithelial–mesenchymal transition after 7 culture days

    SciTech Connect

    Foroni, Laura [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Vasuri, Francesco, E-mail: vasurifrancesco@libero.it [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Chair of Vascular Surgery, Department of Specialistic Surgery and Anaesthesiological Sciences, S. Orsola-Malpighi Hospital, Bologna University (Italy); Valente, Sabrina [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Gualandi, Chiara [Department of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, RU Bologna), Bologna University (Italy); Focarete, Maria Letizia [Department of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, RU Bologna), Bologna University (Italy); Health Science and Technologies–Interdepartmental Center for Industrial Research (HST-ICIR), Bologna University (Italy); Caprara, Giacomo [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Scandola, Mariastella [Department of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, RU Bologna), Bologna University (Italy); D'Errico-Grigioni, Antonia; Pasquinelli, Gianandrea [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy)

    2013-06-10

    We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7. Scanning electron microscopy was performed at culture days 3 and 7. Immunohistochemistry (for E-cadherin, ?-catenin, cytokeratins, nucleophosmin, tubulin, Ki-67 and vimentin), immunofluorescence (for F-actin) western blot (for E-cadherin, ?-catenin and vimentin) and transmission electron microscopy were carried out at day 7. An EMT gene array followed by PCR analysis confirmed the regulation of selected genes. At day 7, scanning electron microscopy on aligned-PLA revealed spindle-shaped cells gathered in buds and ribbon-like structures, with a higher nucleolar/nuclear ratio and a loss in E-cadherin and ?-catenin at immunohistochemistry and western blot. An up-regulation of SMAD2, TGF-?2, TFPI2 and SOX10 was found in aligned-PLA compared to random-PLA cultured cells. The topography of the extracellular matrix has a role in tumor EMT, and a more aggressive phenotype characterizes MCF-7 cells cultured on aligned-PLA scaffold. -- Highlights: • After 7 culture days an aligned-PLA scaffold induces a spindle shape to MCF-7 cells. • Despite these changes, the aligned MCF-7 cells keep an epithelial phenotype. • The extracellular environment alone influences the E-cadherin/?-catenin axis. • The extracellular environment can promote the epithelial–mesenchymal transition.

  20. The response of middle atmospheric ozone to solar UV irradiance variations with a period of 27 days

    NASA Technical Reports Server (NTRS)

    Chen, LI; Brasseur, Guy; London, Julius

    1994-01-01

    A one-dimensional photochemical-dynamical-radiative time-dependent model was used to study the response of middle atmospheric temperature and ozone to solar UV irradiance variations with the period of 27 days. The model solar UV O(x), HO(x), NO(x), and CIO(x)families and modeled solar UV variations. The amplitude of the primary temperature response to the solar UV variation is plus 0.4 K at 85-90 km with a phase lag of about 6 days. A secondary maximum response of plus 0.3 K at 45-50 km appears with a phase lag of 1 day. There is a maximum positive ozone response to the 27-day solar UV oscillation of 2.5 percent at 80-90 km with a phase lag of about 10 days after the solar irradiance maximum. At 70 km the ozone response is about 1.2 percent and is out of phase with the solar variation. In the upper stratosphere (40-50 km) the relative ozone variation is small, about 0.2 percent to 0.3 percent, and there is a negative phase of about 4 days between the ozone and solar oscillations. These oscillations are in phase in the middle stratosphere (35-40 km) where there is again a maximum relative response of about 0.6 percent. The reasons for these ozone amplitude and phase variations are discussed.

  1. 31.4 day periodicity in Cyg X-3 ASM-data: evidence for a tertiary body or precessing disc ?

    NASA Astrophysics Data System (ADS)

    Vilhu, Osmi; Hakala, Pasi; Hannikainen, Diana

    2011-05-01

    The X-ray/gamma-ray activity of the microquasar Cygnus X-3 has been reported in several recent ATELs: #2644 (INTEGRAL), #2645 and #3239 (AGILE), #2646 and #3233 (Fermi LAT). Here we report the finding of a periodic change in the X-ray light curve minimum time (Observed-Computed, O-C) using RXTE/ASM soft X-ray data. The ASM data consisted of 97450 dwell datapoints taken during 5374 days (up to October 2010).

  2. A 6- to 8-day periodicity in cercarial shedding occurred in some Galba truncatula experimentally infected with Fasciola hepatica

    Microsoft Academic Search

    P. Vignoles; N. Alarion; V. Bellet; G. Dreyfuss; D. Rondelaud

    2006-01-01

    A retrospective study on a total of 669 Galba truncatula (three groups) experimentally infected with Fasciola hepatica was carried out to determine why 6- to 8-day interwave intervals, separating the successive waves of cercarial shedding,\\u000a occurred with a regular pattern in some snails during the whole patent period. In the three groups of snails, the number of\\u000a cercariae per shedding

  3. Physical properties of the 0.94-day period transiting planetary system WASP18

    Microsoft Academic Search

    John Southworth; T. C. Hinse; M. Dominik; M. Glitrup; U. G. Jorgensen; C. Liebig; M. Mathiasen; D. R. Anderson; V. Bozza; P. Browne; M. Burgdorf; S. Calchi. Novati; S. Dreizler; F. Finet; K. Harpsoe; F. Hessman; M. Hundertmark; G. Maier; L. Mancini; P. F. L. Maxted; S. Rahvar; D. Ricci; G. Scarpetta; J. Skottfelt; C. Snodgrass; J. Surdej; F. Zimmer

    2009-01-01

    We present high-precision photometry of five consecutive transits of WASP-18,\\u000aan extrasolar planetary system with one of the shortest orbital periods known.\\u000aThrough the use of telescope defocussing we achieve a photometric precision of\\u000a0.47 to 0.83 mmag per observation over complete transit events. The data are\\u000aanalysed using the JKTEBOP code and three different sets of stellar\\u000aevolutionary models.

  4. Vida PURA: A Cultural Adaptation of Screening and Brief Intervention to Reduce Unhealthy Drinking among Latino Day Laborers.

    PubMed

    Ornelas, India J; Allen, Claire; Vaughan, Catalina; Williams, Emily C; Negi, Nalini

    2014-08-25

    ABSTRACT Background: Brief intervention is known to reduce drinking in primary care, however because health care access is limited for Latino immigrants, traditional brief interventions are unlikely to reach this population. Methods: Using Barrera and Castro's framework, our study aims to culturally adapt a screening and brief intervention program to reduce unhealthy alcohol use among Latino day laborers, a particularly vulnerable group of Latino immigrant men. We conducted 18 interviews with Latino day laborers and 13 interviews with mental health and substance use providers that serve Latino immigrant men. Interviews were conducted until saturation of themes was reached. Themes from interviews were used to identify sources of mismatch between traditional screening and brief intervention and our target population. Results: Unhealthy alcohol use was common,culturally accepted, and helped relieve immigration-related stressors. Men had limited knowledge about how to change their behavior. Men preferred to receive information from trusted providers in Spanish. Men faced significant barriers to accessing health and social services, but were open to receiving brief interventions in community settings. Findings were used to design Vida PURA, a preliminary adaptation design of brief intervention for Latino day laborers. Key adaptations include providing brief intervention at a day labor worker center, by promotores trained to incorporate the social and cultural context of drinking for Latino immigrant men. Conclusions: Culturally adapted brief intervention may help reduce unhealthy drinking in this underserved population. PMID:25153904

  5. Clouds in North Polar Region Tracked by MOC Over Five Day Period

    NASA Technical Reports Server (NTRS)

    1999-01-01

    It is mid-summer in the northern hemisphere of Mars--a time of enhanced heating that leads to the release of water vapor into the atmosphere. In the north polar region, temperature differences between bright areas of year-round ice and dark areas of sand and rock create strong winds that mix the atmosphere and create waves of clouds that swirl around the polar cap. Sometimes, as seen during the Viking mission, these winds form tight cyclones; other times, they weave an intricate pattern reflecting the turbulence of the circulation of the atmosphere.

    This animation shows four days of observations of a representative portion of the northern hemisphere. Five Mars Orbiter Camera (MOC) wide angle image pairs(red/blue filters) were combined. These image pairs were warped to create a polar stereographic map projection, which is used by cartographers to present polar areas as if viewed from above. The edges of the pictures move back and forth because of the slightly different path taken by the Mars Global Surveyor spacecraft on each of the five orbits used in this sequence. Seen in the right-hand side of the image is the permanent ice cap and the dark areas that surround it.

    The motion of the clouds viewed in this image is typical for this season on Mars, and shows forms often seen on Earth. Waves of clouds are moving from the upper portion of the frame towards the bottom (towards the east northeast). This motion is most likely the movement of a moister portion of the martian atmosphere under the influence of circumpolar winds. Early in the sequence, a prominent circular band of clouds moves almost due east, rotating slightly counter-clockwise. Towards the end of the sequence, the circle dissipates and a linear set of clouds propagates towards the bottom of the frame. Linear cloud speeds vary from day today, averaging about 16 km/hr (10 miles/hr); rotational rates appear to have been less than 10 km/hr (6 miles/hr).

    Malin Space Science Systems and the California Institute of Technology built the MOC using spare hardware from the Mars Observer mission. MSSS operates the camera from its facilities in San Diego, CA. The Jet Propulsion Laboratory's Mars Surveyor Operations Project operates the Mars Global Surveyor spacecraft with its industrial partner, Lockheed Martin Astronautics, from facilities in Pasadena, CA and Denver, CO.

  6. PHYSICAL PROPERTIES OF THE 0.94-DAY PERIOD TRANSITING PLANETARY SYSTEM WASP-18

    SciTech Connect

    Southworth, John; Anderson, D. R.; Maxted, P. F. L. [Astrophysics Group, Keele University, Newcastle-under Lyme, ST5 5BG (United Kingdom); Hinse, T. C. [Armagh Observatory, College Hill, Armagh, BT61 9DG (United Kingdom); Dominik, M.; Mathiasen, M.; Browne, P. [SUPA, University of St. Andrews, School of Physics and Astronomy, North Haugh, St. Andrews, KY16 9SS (United Kingdom); Glitrup, M. [Department of Physics and Astronomy, Aarhus University, Ny Munkegade, 8000 Aarhus C (Denmark); Joergensen, U. G.; Harpsoee, K. [Niels Bohr Institute and Centre for Star and Planet Formation, University of Copenhagen, Juliane Maries vej 30, 2100 Copenhagen Oe (Denmark); Liebig, C.; Maier, G. [Astronomisches Rechen-Institut, Zentrum fuer Astronomie, Universitaet Heidelberg, Moenchhofstrasse 12-14, 69120 Heidelberg (Germany); Bozza, V.; Calchi Novati, S.; Mancini, L. [Dipartimento di Fisica 'E. R. Caianiello', Universita di Salerno, Baronissi (Italy); Burgdorf, M. [Deutsches SOFIA Institut, NASA Ames Research Center, Mail Stop 211-3, Moffett Field, CA 94035 (United States); Dreizler, S.; Hessman, F.; Hundertmark, M. [Institut fuer Astrophysik, Georg-August-Universitaet Goettingen, Friedrich-Hund-Platz 1, 37077 Goettingen (Germany); Finet, F., E-mail: jkt@astro.keele.ac.u [Institut d'Astrophysique et de Geophysique, Universite de Liege, 4000 Liege (Belgium)

    2009-12-10

    We present high-precision photometry of five consecutive transits of WASP-18, an extrasolar planetary system with one of the shortest orbital periods known. Through the use of telescope defocusing we achieve a photometric precision of 0.47-0.83 mmag per observation over complete transit events. The data are analyzed using the JKTEBOP code and three different sets of stellar evolutionary models. We find the mass and radius of the planet to be M {sub b} = 10.43 +- 0.30 +- 0.24 M {sub Jup} and R {sub b} = 1.165 +- 0.055 +- 0.014 R {sub Jup} (statistical and systematic errors), respectively. The systematic errors in the orbital separation and the stellar and planetary masses, arising from the use of theoretical predictions, are of a similar size to the statistical errors and set a limit on our understanding of the WASP-18 system. We point out that seven of the nine known massive transiting planets (M {sub b} > 3 M {sub Jup}) have eccentric orbits, whereas significant orbital eccentricity has been detected for only four of the 46 less-massive planets. This may indicate that there are two different populations of transiting planets, but could also be explained by observational biases. Further radial velocity observations of low-mass planets will make it possible to choose between these two scenarios.

  7. 35 day cycle and the rotation period of HER X-1

    NASA Astrophysics Data System (ADS)

    Oegelman, H.; Kahabka, P.; Pietsch, W.; Truemper, J.; Voges, W.

    1985-02-01

    Sampling observations of a complete 35d cycle of Her X-1 during March 1, to April 5, 1984 with the low and medium energy X-ray detectors of EXOSAT are presented. The intensities measured in the Argon detectors are compared with the UHURU 35d light curve to obtain the turn-on times of two consecutive cycles as JD 2445753.0 + or - 1.0, and JD 2445788.0 + or - 0.5. The low energy data is used to determine the rotation period and its derivative as: 1.23779200 s + or 50 ns, and (-2 + or - 1) x 10 to the -13th respectively, at the epoch JD 2445778.56. Analysis of the pulse arrival phases indicate that during mid-on phase of the 35d cycle there is shift of about 180 deg. Evidence for the long term correlated changes of P35 and P1.24s is presented, confirming the low quality of the 35d clock and suggesting that the basic clock mechanism must lie in the disk structure itself.

  8. Spin-orbit alignment for 110 day period KOI368.01 from gravity darkening

    SciTech Connect

    Ahlers, John P.; Seubert, Shayne A.; Barnes, Jason W. [Department of Physics, University of Idaho, 875 Perimeter Drive Stop 440903, Moscow, ID 83844-0903 (United States)

    2014-05-10

    We fit the Kepler photometric light curve of the KOI-368 system using an oblate, gravity-darkened stellar model in order to constrain its spin-orbit alignment. We find that the system is relatively well-aligned with a sky-projected spin-orbit alignment of ? = 10° ± 2°, a stellar obliquity of ? = 3° ± 7°, and a true spin-orbit alignment of ? = 11° ± 3°. Although our measurement differs significantly from zero, the low value for ? is consistent with spin-orbit alignment. We also measure various transit parameters of the KOI-368 system: R {sub KOI-368} = 2.28 ± 0.02 R {sub ?}, R{sub p} = 1.83 ± 0.02 R {sub jup}, and i = 89.°221 ± 0.°013. This work shows that our gravity-darkened model can constrain long-period, well-aligned planets and M-class stars orbiting fast-rotators, allowing for measurement of a new subcategory of transiting bodies.

  9. Planktonic and biofilm community characterization and Salmonella resistance of 14-day old chicken cecal microflora derived continuous-flow cultures

    Technology Transfer Automated Retrieval System (TEKTRAN)

    These studies were initiated to compare the composition of GIT bacterial communities in birds during the transition period in age where their susceptibility to Salmonella shifts to resistance. One of the challenges to developing probiotics is to develop an efficacious culture of minimal diversity, ...

  10. Global Characteristics of the Correlation and Time Lag Between Solar and Ionospheric Parameters in the 27-day Period

    NASA Technical Reports Server (NTRS)

    Lee, Choon-Ki; Han, Shin-Chan; Dieter,Bilitza; Ki-Weon,Seo

    2012-01-01

    The 27-day variations of topside ionosphere are investigated using the in-situ electron density measurements from the CHAMP planar Langmuir probe and GRACE K-band ranging system. As the two satellite systems orbit at the altitudes of approx. 370 km and approx. 480 km, respectively, the satellite data sets are greatly valuable for examining the electron density variations in the vicinity of F2-peak. In a 27-day period, the electron density measurements from the satellites are in good agreements with the solar flux, except during the solar minimum period. The time delays are mostly 1-2 day and represent the hemispherical asymmetry. The globally-estimated spatial patterns of the correlation between solar flux and in-situ satellite measurements show poor correlations in the (magnetic) equatorial region, which are not found from the ground measurements of vertically-integrated electron content. We suggest that the most plausible cause for the poor correlation is the vertical movement of ionization due to atmospheric dynamic processes that is not controlled by the solar extreme ultraviolet radiation.

  11. The Day Bolivian Students Came to School Motivating Students through Culture

    ERIC Educational Resources Information Center

    Davin, Kristin

    2010-01-01

    Like many elementary foreign language teachers, the author traveled from room to room to teach. Each room had different materials and a different classroom culture. This article describes how the author taught her students about Bolivia and how to motivate them through culture. It discusses a service-learning project that brings life-changing…

  12. Factors influencing the chance of cows being pregnant 30 days after the herd voluntary waiting period.

    PubMed

    Löf, E; Gustafsson, H; Emanuelson, U

    2014-04-01

    The objective of this study was to study factors affecting a reproductive performance indicator at the cow level adjusted for herd management strategy. Associations between the outcome variable, pregnant or not at the herd voluntary waiting period (VWP) plus 30d (pregnant at VWP+30), and the predictor variables were analyzed using a multivariable, generalized estimation equations model that adjusted for clustering of the data at the herd level. The statistical analysis was stratified on parity. In total, 132,721 cows were retained for analyses, of which 29,113 (22%) were pregnant at VWP+30d. Of the nonpregnant cows, 81,483 cows had records of artificial inseminations (AI) and 22,125 cows had no records of AI. The chance of pregnancy was higher for cows of the Swedish Red and for other/crossbreeds compared with Swedish Holstein, for cows from herds with high heat detection efficiency compared with cows from herds with medium and low heat detection efficiency, for cows from herds with long VWP (i.e., >51d) compared with cows from herds with short VWP (<51d), and for cows in freestalls compared with cows in tiestalls. The chance for pregnancy was lower for cows with severe problems at claw trimming compared with cows with no problems at trimming (only for second- and higher-parity cows), for cows that had a record of reproduction-related disease, for cows that had a record of any other disease compared with cows without record, for second- and higher-parity cows with records of dystocia compared with cows with no record of dystocia, for first-parity cows in the group with the highest milk yield compared with first-parity cows in the group with the lowest milk yield, for cows of third and higher parity in the group with the lowest milk yield compared with cows in higher yielding groups, for cows bred in summer compared with those bred in winter-spring (not significant for first-parity cows), and for cows with a twin birth had compared with cows with a single birth. We observed associations of the dose-response type, such that when the milk fat-to-protein ratio increased, the chance for pregnancy decreased, and as the somatic cell count increased, the chance for pregnancy decreased. In conclusion, factors that are known to affect reproductive efficiency also affect the chance of cows being pregnant at the herd VWP plus 30d. PMID:24485688

  13. Cultural/Favorite Recipe Day: Strengthening Approaches to Increase Culturally Diverse Foods Served in Head Start Meals

    ERIC Educational Resources Information Center

    Hoffman, Jessica A.; Agrawal, Tara; Carter, Sonia; Grinder, AnnMarie; Castaneda-Sceppa, Carmen

    2012-01-01

    One approach to halting the childhood obesity epidemic has been the modification of foods available to children during the school day. In recent years there has been an increased focus on obesity prevention efforts among children ages birth to 5 and the role of child care settings in prevention efforts. Head Start serves as an important venue for…

  14. 76 FR 16033 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...Culture Evaluation IVLP Survey. OMB Control Number: None. Type of Request: New Collection. Originating Office: Bureau...generated as needed. Respondents: International Visitor Leadership Program (IVLP) exchange program participants from...

  15. Primitive lymphohematopoietic precursor cell lines generated in culture from day 7 early-mid-primitive streak stage mouse embryo.

    PubMed Central

    Palacios, R; Imhof, B A

    1996-01-01

    During mouse development, the first lymphohematopoietic precursor cells and myeloid or erythroid cell lineage-determined cells can be detected in the yolk sac at days 8-8.5 of gestation. The characteristics of the cells that give rise to these yolk sac primitive lymphohematopoietic cells and the molecular events controlling this process remain poorly defined. We show here that cell suspensions from day 7 early-mid-primitive streak stage embryo proper generated early immature PgP-1+ Joro 177+ Lin- hematopoietic cells and some Mac-1+ myeloid and TER 119+ erythroid cells after co-culture with the yolk sac-derived stromal cell line YS6 without addition of exogenous cytokines. Purified Lin- hematopoietic cells generated in these cultures did not express genes known to be transcribed at early stages of lymphoid, myeloid or erythroid cell differentiation and were able to give rise to T and B lymphocytes, myeloid cells and erythroid cells after appropriate further induction in vitro. Several cell lines were established in culture with a mixture of four cytokines from the PgP-1+ Joro 177+ Lin- cell population. The cell lines shared phenotypic and genotypic characteristics with the PgP-1+ Joro 177+ Lin- cell population generated in culture from day 7 embryo proper and they were able to reconstitute the lymphohematopoietic system of irradiated mice. Taken together these results support a model of lymphohematopoiesis in which cells from day 7 early-mid-primitive streak mouse embryo proper migrate and colonize the visceral yolk sac. There they generate primitive lymphohematopoietic precursor cells and the first erythroid and myeloid hematopoietic cells under the influence of yolk sac stromal cells like the YS6 cells described here. Images PMID:9003763

  16. Use of a lactobacillus-based probiotic culture to reduce Salmonella in day of hatch broilers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A commercially available Lactobacillus probiotic (FM-B11™ Ivesco LLC) (B11) significantly reduced Salmonella recovery from day-of-hatch chicks in several studies. For all experiments, day-of-hatch male broiler chicks (n=40 per pen) were challenged with approximately 10**4 cfu per chick of Salmonell...

  17. Block training periodization in alpine skiing: effects of 11-day HIT on VO2max and performance.

    PubMed

    Breil, Fabio A; Weber, Simone N; Koller, Stefan; Hoppeler, Hans; Vogt, Michael

    2010-08-01

    Attempting to achieve the high diversity of training goals in modern competitive alpine skiing simultaneously can be difficult and may lead to compromised overall adaptation. Therefore, we investigated the effect of block training periodization on maximal oxygen consumption (VO2max) and parameters of exercise performance in elite junior alpine skiers. Six female and 15 male athletes were assigned to high-intensity interval (IT, N = 13) or control training groups (CT, N = 8). IT performed 15 high-intensity aerobic interval (HIT) sessions in 11 days. Sessions were 4 x 4 min at 90-95% of maximal heart rate separated by 3-min recovery periods. CT continued their conventionally mixed training, containing endurance and strength sessions. Before and 7 days after training, subjects performed a ramp incremental test followed by a high-intensity time-to-exhaustion (tlim) test both on a cycle ergometer, a 90-s high-box jump test as well as countermovement (CMJ) and squat jumps (SJ) on a force plate. IT significantly improved relative VO2max by 6.0% (P < 0.01; male +7.5%, female +2.1%), relative peak power output by 5.5% (P < 0.01) and power output at ventilatory threshold 2 by 9.6% (P < 0.01). No changes occurred for these measures in CT. tlim remained unchanged in both groups. High-box jump performance was significantly improved in males of IT only (4.9%, P < 0.05). Jump peak power (CMJ -4.8%, SJ -4.1%; P < 0.01), but not height decreased in IT only. For competitive alpine skiers, block periodization of HIT offers a promising way to efficiently improve VO2max and performance. Compromised explosive jump performance might be associated with persisting muscle fatigue. PMID:20364385

  18. Comprehensive Mapping of Regional Expression of the Clock Protein PERIOD2 in Rat Forebrain across the 24-h Day

    PubMed Central

    Harbour, Valerie L.; Weigl, Yuval; Robinson, Barry; Amir, Shimon

    2013-01-01

    In mammals, a light-entrainable clock located in the suprachiasmatic nucleus (SCN) regulates circadian rhythms by synchronizing oscillators throughout the brain and body. Notably, the nature of the relation between the SCN clock and subordinate oscillators in the rest of the brain is not well defined. We performed a high temporal resolution analysis of the expression of the circadian clock protein PERIOD2 (PER2) in the rat forebrain to characterize the distribution, amplitude and phase of PER2 rhythms across different regions. Eighty-four LEW/Crl male rats were entrained to a 12-h: 12-h light/dark cycle, and subsequently perfused every 30 min across the 24-h day for a total of 48 time-points. PER2 expression was assessed with immunohistochemistry and analyzed using automated cell counts. We report the presence of PER2 expression in 20 forebrain areas important for a wide range of motivated and appetitive behaviors including the SCN, bed nucleus, and several regions of the amygdala, hippocampus, striatum, and cortex. Eighteen areas displayed significant PER2 rhythms, which peaked at different times of day. Our data demonstrate a previously uncharacterized regional distribution of rhythms of a clock protein expression in the brain that provides a sound basis for future studies of circadian clock function in animal models of disease. PMID:24124556

  19. Effects of a Seven Day Overload-Period of High-Intensity Training on Performance and Physiology of Competitive Cyclists

    PubMed Central

    Clark, Bradley; Costa, Vitor P.; O'Brien, Brendan J.; Guglielmo, Luiz G.; Paton, Carl D.

    2014-01-01

    Objectives Competitive endurance athletes commonly undertake periods of overload training in the weeks prior to major competitions. This investigation examined the effects of two seven-day high-intensity overload training regimes (HIT) on performance and physiological characteristics of competitive cyclists. Design The study was a matched groups, controlled trial. Methods Twenty-eight male cyclists (mean ± SD, Age: 33±10 years, Mass 74±7 kg, VO2 peak 4.7±0.5 L·min?1) were assigned to a control group or one of two training groups for seven consecutive days of HIT. Before and after training cyclists completed an ergometer based incremental exercise test and a 20-km time-trial. The HIT sessions were ?120 minutes in duration and consisted of matched volumes of 5, 10 and 20 second (short) or 15, 30 and 45 second (long) maximal intensity efforts. Results Both the short and long HIT regimes led to significant (p<0.05) gains in time trial performance compared to the control group. Relative to the control group, the mean changes (±90% confidence limits) in time-trial power were 8.2%±3.8% and 10.4%±4.3% for the short and long HIT regimes respectively; corresponding increases in peak power in the incremental test were 5.5%±2.7% and 9.5%±2.5%. Both HIT (short vs long) interventions led to non-significant (p>0.05) increases (mean ± SD) in VO2 peak (2.3%±4.7% vs 3.5%±6.2%), lactate threshold power (3.6%±3.5% vs 2.9%±5.3%) and gross efficiency (3.2%±2.4% vs 5.1%±3.9%) with only small differences between HIT regimes. Conclusions Seven days of overload HIT induces substantial enhancements in time-trial performance despite non-significant increases in physiological measures with competitive cyclists. PMID:25521824

  20. Postnatal Day 2 to 11 Constitutes a 5-HT-Sensitive Period Impacting Adult mPFC Function

    PubMed Central

    Rebello, Tahilia J.; Yu, Qinghui; Goodfellow, Nathalie M.; Caffrey Cagliostro, Martha K.; Teissier, Anne; Morelli, Emanuela; Demireva, Elena Y.; Chemiakine, Alexei; Rosoklija, Gorazd B.; Dwork, Andrew J.; Lambe, Evelyn K.; Ansorge, Mark S.

    2014-01-01

    Early-life serotonin [5-hydroxytryptamine (5-HT)] signaling modulates brain development, which impacts adult behavior, but 5-HT-sensitive periods, neural substrates, and behavioral consequences remain poorly understood. Here we identify the period ranging from postnatal day 2 (P2) to P11 as 5-HT sensitive, with 5-HT transporter (5-HTT) blockade increasing anxiety- and depression-like behavior, and impairing fear extinction learning and memory in adult mice. Concomitantly, P2–P11 5-HTT blockade causes dendritic hypotrophy and reduced excitability of infralimbic (IL) cortex pyramidal neurons that normally promote fear extinction. By contrast, the neighboring prelimbic (PL) pyramidal neurons, which normally inhibit fear extinction, become more excitable. Excitotoxic IL but not PL lesions in adult control mice reproduce the anxiety-related phenotypes. These findings suggest that increased 5-HT signaling during P2–P11 alters adult mPFC function to increase anxiety and impair fear extinction, and imply a differential role for IL and PL neurons in regulating affective behaviors. Together, our results support a developmental mechanism for the etiology and pathophysiology of affective disorders and fear-related behaviors. PMID:25209278

  1. Postnatal day 2 to 11 constitutes a 5-HT-sensitive period impacting adult mPFC function.

    PubMed

    Rebello, Tahilia J; Yu, Qinghui; Goodfellow, Nathalie M; Caffrey Cagliostro, Martha K; Teissier, Anne; Morelli, Emanuela; Demireva, Elena Y; Chemiakine, Alexei; Rosoklija, Gorazd B; Dwork, Andrew J; Lambe, Evelyn K; Gingrich, Jay A; Ansorge, Mark S

    2014-09-10

    Early-life serotonin [5-hydroxytryptamine (5-HT)] signaling modulates brain development, which impacts adult behavior, but 5-HT-sensitive periods, neural substrates, and behavioral consequences remain poorly understood. Here we identify the period ranging from postnatal day 2 (P2) to P11 as 5-HT sensitive, with 5-HT transporter (5-HTT) blockade increasing anxiety- and depression-like behavior, and impairing fear extinction learning and memory in adult mice. Concomitantly, P2-P11 5-HTT blockade causes dendritic hypotrophy and reduced excitability of infralimbic (IL) cortex pyramidal neurons that normally promote fear extinction. By contrast, the neighboring prelimbic (PL) pyramidal neurons, which normally inhibit fear extinction, become more excitable. Excitotoxic IL but not PL lesions in adult control mice reproduce the anxiety-related phenotypes. These findings suggest that increased 5-HT signaling during P2-P11 alters adult mPFC function to increase anxiety and impair fear extinction, and imply a differential role for IL and PL neurons in regulating affective behaviors. Together, our results support a developmental mechanism for the etiology and pathophysiology of affective disorders and fear-related behaviors. PMID:25209278

  2. Cultural techniques for altering the flowering time and double-cropping short-day varieties

    Technology Transfer Automated Retrieval System (TEKTRAN)

    July-plugged transplants of short-day cv. Strawberry Festival (Fragaria x ananassa), flowered in October and November even though they were grown under long photoperiods and warm temperatures (greater than 21 degrees C) in July and August. These unexpected results were attributed to a high plant de...

  3. Culture and Character Education in a Jewish Day School: A Case Study of Life and Experience

    ERIC Educational Resources Information Center

    Roso, Calvin G.

    2013-01-01

    This article addresses how to teach character comprehensively by studying ways a school's concurrent curricula (the official curriculum, the operational curriculum, the extra curriculum, and the hidden curriculum) can be used to teach character to students. A single case study analyzes the curriculum at a Jewish day school by examining school…

  4. Transcript abundance and apoptosis in day-7 porcine blastocyst cultured with exogenous insulin-like growth factor-I.

    PubMed

    Wasielak, Marta; Fujii, Takashi; Ohsaki, Tsubasa; Hashizume, Tsutomu; Bogacki, Marek; Sawai, Ken

    2013-03-01

    Exogenous growth factors may increase the efficiency of embryo development in vitro. The aim of the present study was to examine the effects of insulin-like growth factor (IGF)-I on porcine embryo development. Porcine embryos obtained by in vitro fertilization were cultured for seven days in the presence of IGF-I (50, 100 or 150ng/ml). Subsequently, relative transcript abundance (RA) of IGF-related genes (IGFR1, IGFBP2, and IGFBP3), glucose transporter genes (SLC2A4 and SLC2A8), and apoptosis-related genes (BAX and BCL-XL) was analyzed. No differences were observed in the cleavage rate on day 2 post insemination (pi) and blastocysts rate on day 7pi between IGF-treated and control embryos. IGF-I treatment did not affect RA of IGFR1, IGFBP3, and SLC2A4 genes, but decreased RA of IGFBP2 and SLC2A8 genes. The percentage of TUNEL-positive nuclei in blastocysts did not differ between the experimental groups. However, RA of BAX and BCL-XL genes decreased in response to all IGF-I concentrations, whereas the BCL-XL/BAX RA ratio was enhanced when embryos were cultured in medium containing 150ng/ml of IGF-I. These results indicate that IGF-I did not stimulate in vitro development of porcine embryos through the IGF signaling system, nor did IGF-I stimulate RA of glucose transporter genes. However, IGF-I at the highest dose was able to increase the BCL-XL/BAX transcript expression ratio. This may indicate that the primary role of IGF-I during the first days of embryo development in the pig is associated with anti-apoptotic actions rather than with growth stimulation. PMID:23522072

  5. 1036 Federal Register / Vol. 55, No. 8 / Thursday, January 11, 1990 / Rules and Regalations limited to a period of 45 days. After 45

    E-print Network

    to a period of 45 days. After 45 days if an increase is still needed, a carrier can renew the existing tariff increase or publish a new tariff. [FR Doc. 90-736 Filed 1-10-90; 8:45 am] BILUNG COOE 703-01-M DEPARTMENT

  6. Robust circadian rhythms in organoid cultures from PERIOD2::LUCIFERASE mouse small intestine.

    PubMed

    Moore, Sean R; Pruszka, Jill; Vallance, Jefferson; Aihara, Eitaro; Matsuura, Toru; Montrose, Marshall H; Shroyer, Noah F; Hong, Christian I

    2014-09-01

    Disruption of circadian rhythms is a risk factor for several human gastrointestinal (GI) diseases, ranging from diarrhea to ulcers to cancer. Four-dimensional tissue culture models that faithfully mimic the circadian clock of the GI epithelium would provide an invaluable tool to understand circadian regulation of GI health and disease. We hypothesized that rhythmicity of a key circadian component, PERIOD2 (PER2), would diminish along a continuum from ex vivo intestinal organoids (epithelial 'miniguts'), nontransformed mouse small intestinal epithelial (MSIE) cells and transformed human colorectal adenocarcinoma (Caco-2) cells. Here, we show that bioluminescent jejunal explants from PERIOD2::LUCIFERASE (PER2::LUC) mice displayed robust circadian rhythms for >72 hours post-excision. Circadian rhythms in primary or passaged PER2::LUC jejunal organoids were similarly robust; they also synchronized upon serum shock and persisted beyond 2 weeks in culture. Remarkably, unshocked organoids autonomously synchronized rhythms within 12 hours of recording. The onset of this autonomous synchronization was slowed by >2 hours in the presence of the glucocorticoid receptor antagonist RU486 (20 ?M). Doubling standard concentrations of the organoid growth factors EGF, Noggin and R-spondin enhanced PER2 oscillations, whereas subtraction of these factors individually at 24 hours following serum shock produced no detectable effects on PER2 oscillations. Growth factor pulses induced modest phase delays in unshocked, but not serum-shocked, organoids. Circadian oscillations of PER2::LUC bioluminescence aligned with Per2 mRNA expression upon analysis using quantitative PCR. Concordant findings of robust circadian rhythms in bioluminescent jejunal explants and organoids provide further evidence for a peripheral clock that is intrinsic to the intestinal epithelium. The rhythmic and organotypic features of organoids should offer unprecedented advantages as a resource for elucidating the role of circadian rhythms in GI stem cell dynamics, epithelial homeostasis and disease. PMID:24997189

  7. Robust circadian rhythms in organoid cultures from PERIOD2::LUCIFERASE mouse small intestine

    PubMed Central

    Moore, Sean R.; Pruszka, Jill; Vallance, Jefferson; Aihara, Eitaro; Matsuura, Toru; Montrose, Marshall H.; Shroyer, Noah F.; Hong, Christian I.

    2014-01-01

    Disruption of circadian rhythms is a risk factor for several human gastrointestinal (GI) diseases, ranging from diarrhea to ulcers to cancer. Four-dimensional tissue culture models that faithfully mimic the circadian clock of the GI epithelium would provide an invaluable tool to understand circadian regulation of GI health and disease. We hypothesized that rhythmicity of a key circadian component, PERIOD2 (PER2), would diminish along a continuum from ex vivo intestinal organoids (epithelial ‘miniguts’), nontransformed mouse small intestinal epithelial (MSIE) cells and transformed human colorectal adenocarcinoma (Caco-2) cells. Here, we show that bioluminescent jejunal explants from PERIOD2::LUCIFERASE (PER2::LUC) mice displayed robust circadian rhythms for >72 hours post-excision. Circadian rhythms in primary or passaged PER2::LUC jejunal organoids were similarly robust; they also synchronized upon serum shock and persisted beyond 2 weeks in culture. Remarkably, unshocked organoids autonomously synchronized rhythms within 12 hours of recording. The onset of this autonomous synchronization was slowed by >2 hours in the presence of the glucocorticoid receptor antagonist RU486 (20 ?M). Doubling standard concentrations of the organoid growth factors EGF, Noggin and R-spondin enhanced PER2 oscillations, whereas subtraction of these factors individually at 24 hours following serum shock produced no detectable effects on PER2 oscillations. Growth factor pulses induced modest phase delays in unshocked, but not serum-shocked, organoids. Circadian oscillations of PER2::LUC bioluminescence aligned with Per2 mRNA expression upon analysis using quantitative PCR. Concordant findings of robust circadian rhythms in bioluminescent jejunal explants and organoids provide further evidence for a peripheral clock that is intrinsic to the intestinal epithelium. The rhythmic and organotypic features of organoids should offer unprecedented advantages as a resource for elucidating the role of circadian rhythms in GI stem cell dynamics, epithelial homeostasis and disease. PMID:24997189

  8. 43 CFR 1822.14 - What if I try to file a required document on the last day of the stated period for filing, but...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...period for filing, but the BLM office where it is to be filed is officially closed all...period for filing, but the BLM office where it is to be filed is officially closed all...considers the document timely filed if we receive it in the office on the next day it is...

  9. CTEPP-OH DATA COLLECTED ON FORM 10 (PERIODS 1-3): DAY CARE CENTER CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data set contains data for CTEPP-OH concerning the child?s activities at the day care center over the 48-h monitoring period. The diary was divided into three time periods over the 48-h monitoring interval. The Food Survey collected information on the frequency and types of ...

  10. Valepotriate content in different in vitro cultures of Valerianaceae and characterization of Valeriana officinalis L. callus during a growth period.

    PubMed

    Violon, C; Van Cauwenbergh, N; Vercruysse, A

    1983-10-21

    Different in vitro cultures of Valerianaceae were analysed for valepotriate content [(iso)valtrate, acevaltrate, didrovaltrate] in a study on properties of production in vitro (plant species, growth conditions, differentiation level, valepotriate content of the medium after growth). The in vitro cultures were: callus cultures of Valeriana officinalis L., Valerianella locusta L. and Centranthus ruber L.DC.; a suspension culture of Valeriana officinalis L. and a root organ culture of Centranthus ruber L.DC. All of the cultures produced valepotriates in vitro in different amounts. None of the media that had served for growth contained any valepotriates. In order to characterize the in vitro growth more precisely different parameters were analysed at different time intervals during a growth period in one of the cultures (callus culture of Valeriana officinalis L.). These different parameters were: fresh and dry weight, lipid and nitrogen content and (iso)valtrate content. This study during a growth period was performed on two media differing in plant hormone content. PMID:6646985

  11. Short-day and long-day expression patterns of genes involved in the flesh fly clock mechanism: period, timeless, cycle and cryptochrome

    Microsoft Academic Search

    Shin G Goto; David L Denlinger

    2002-01-01

    Though our knowledge of the molecular details of the circadian clock has advanced rapidly, the functional elements of the photoperiodic clock remain largely unknown. As a first step to approach this issue, we report here the sequences and expression patterns of period (per), timeless (tim), cycle (cyc) and cryptochrome (cry) mRNAs in the flesh fly Sarcophaga crassipalpis. Nucleotide and deduced

  12. Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures

    PubMed Central

    Niapour, Nazila; Mohammadi-Ghalehbin, Behnam; Golmohammadi, Mohammad Ghasem; Amani, Mohammad; Salehi, Hossein; Niapour, Ali

    2015-01-01

    Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve. Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The total cell yield and the purity of SCs were evaluated in each group on the first and seventh day after plating. Results: The maximum and minimum numbers of cells were counted in 15 day-long predegene-ration and control groups which underwent no predegeneration. The 10 day-long in vitro predegeneration group with 80±0.5% SCs enrichment had the best purity after plating day and could maintain its purity with elapsing on cultures. Conclusion: 10 day-long predegeneration results in the higher cell number and the better and prolonged purity of SCs in culture. PMID:25945245

  13. Investigation of near-infrared spectroscopy for periodic in situ cell culture media determination of glucose and lactate

    E-print Network

    Lewis, Christopher Bryan

    1999-01-01

    the 2.0-2.5 []m combination region. A system was developed using a Fourier Transform Infrared (FHR) spectrometer to characterize glucose and lactate single beam spectra collected from cell culture media. Spectra recorded from a three-day fibroblast...

  14. Investigation of near-infrared spectroscopy for periodic in situ cell culture media determination of glucose and lactate 

    E-print Network

    Lewis, Christopher Bryan

    1999-01-01

    the 2.0-2.5 []m combination region. A system was developed using a Fourier Transform Infrared (FHR) spectrometer to characterize glucose and lactate single beam spectra collected from cell culture media. Spectra recorded from a three-day fibroblast...

  15. CAN THE 62 DAY X-RAY PERIOD OF ULX M82 X-1 BE DUE TO A PRECESSING ACCRETION DISK?

    SciTech Connect

    Pasham, Dheeraj R. [Astronomy Department, University of Maryland, College Park, MD 20742 (United States); Strohmayer, Tod E., E-mail: dheeraj@astro.umd.edu, E-mail: tod.strohmayer@nasa.gov [Astrophysics Science Division, NASA's Goddard Space Flight Center, Greenbelt, MD 20771 (United States)

    2013-09-10

    We have analyzed all archival Rossi X-Ray Timing Explorer/Proportional Counter Array monitoring observations of the ultraluminous X-ray source M82 X-1 in order to study the properties of its 62 day X-ray period, which was found by Kaaret and Feng in 2007. Based on its high coherence, it has been argued that the observed period is the orbital period of the binary. Utilizing a much longer data set than in previous studies, we find the following. (1) The phase-resolved X-ray (3-15 keV) spectra-modeled with a thermal accretion disk and a power law-suggest that the accretion disk's contribution to the total flux is strongly modulated with phase. (2) Suggestive evidence for a sudden phase shift of approximately 0.4 in phase (25 days) between the first and the second halves of the light curve separated by roughly 1000 days. If confirmed, the implied timescale to change the period is {approx}10 yr, which is exceptionally fast for an orbital phenomenon. These two independent pieces of evidence are consistent with the periodicity being due to a precessing accretion disk, similar to the super-orbital periods observed in systems like Her X-1, LMC X-4, and SS433. However, the timing evidence for a change in the period needs to be confirmed with additional observations. This should be possible with further monitoring of M82 with instruments such as the Swift X-Ray Telescope.

  16. Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets.

    PubMed

    Chen, Qi; Gauger, Phillip; Stafne, Molly; Thomas, Joseph; Arruda, Paulo; Burrough, Eric; Madson, Darin; Brodie, Joseph; Magstadt, Drew; Derscheid, Rachel; Welch, Michael; Zhang, Jianqiang

    2015-08-01

    Porcine deltacoronavirus (PDCoV) was first identified in Hong Kong in 2009-2010 and reported in United States swine for the first time in February 2014. However, diagnostic tools other than polymerase chain reaction for PDCoV detection were lacking and Koch?s postulates had not been fulfilled to confirm the pathogenic potential of PDCoV. In the present study, PDCoV peptide-specific rabbit antisera were developed and used in immunofluorescence and immunohistochemistry assays to assist PDCoV diagnostics. The pathogenicity and pathogenesis of PDCoV was investigated following orogastric inoculation of 5-day-old piglets with a plaque-purified PDCoV cell culture isolate (3×10(4) TCID50 per pig). The PDCoV-inoculated piglets developed mild to moderate diarrhea, shed increasing amount of virus in rectal swabs from 2 to 7 days post inoculation, and developed macroscopic and microscopic lesions in small intestines with viral antigen confirmed by immunohistochemistry staining. This study experimentally confirmed PDCoV pathogenicity and characterized PDCoV pathogenesis in neonatal piglets. PMID:25817405

  17. Cultural Identities of Adolescent Immigrants: A Three-Year Longitudinal Study Including the Pre-Migration Period

    ERIC Educational Resources Information Center

    Tartakovsky, Eugene

    2009-01-01

    This article investigates the cultural identities of adolescent immigrants in the pre-migration period and during the first 3 years after immigration. The target population consists of high-school Jewish adolescents from Russia and Ukraine participating in an Israeli immigration program. In this program, Jewish adolescents immigrate to Israel…

  18. Can the 62 Day X-ray Period of ULX M82 X-1 Be Due to a Precessing Accretion Disk?

    NASA Technical Reports Server (NTRS)

    Pasham, Dheeraj R.; Strohmayer, Tod E.

    2013-01-01

    We have analyzed all the archival RXTE/PCA monitoring observations of the ultraluminous X-ray source (ULX) M82 X-1 in order to study the properties of its previously discovered 62 day X-ray period (Kaaret & Feng 2007). Based on the high coherence of the modulation it has been argued that the observed period is the orbital period of the binary. Utilizing a much longer data set than in previous studies we find: (1) The phase-resolved X-ray (3-15 keV) energy spectra - modeled with a thermal accretion disk and a power-law corona - suggest that the accretion disk's contribution to the total flux is responsible for the overall periodic modulation while the power-law flux remains approximately constant with phase. (2) Suggestive evidence for a sudden phase shift-of approximately 0.3 in phase (20 days)-between the first and the second halves of the light curve separated by roughly 1000 days. If confirmed, the implied timescale to change the period is approx. = 10 yrs, which is exceptionally fast for an orbital phenomenon. These independent pieces of evidence are consistent with the 62 day period being due to a precessing accretion disk, similar to the so-called super-orbital periods observed in systems like Her X-1, LMC X-4, and SS433. However, the timing evidence for a change in the period needs to be confirmed with additional observations. This should be possible with further monitoring of M82 with instruments such as the X-ray telescope (XRT) on board Swift.

  19. The influence of culture method and culture period on quality of half-pearls (‘mabé’) from the winged pearl oyster Pteria sterna, Gould, 1851

    Microsoft Academic Search

    Hugo Ruiz-Rubio; Héctor Acosta-Salmón; Alex Olivera; Paul C. Southgate; Carlos Rangel-Dávalos

    2006-01-01

    Two groups of 96 winged pearl oysters, Pteria sterna, with mean (±S.D.) dorso-ventral shell height (DVM) of 75.0±6.0 mm, were used to evaluate the effect of two culture methods (pocket nets and plastic cages) on the quality of half-pearls (mabé) produced from them over a period of 9 months. Prior to implantation, oysters were anaesthetized using benzocaine and two plastic

  20. Poroelastic behaviour of the degenerating human intervertebral disc: a ten-day study in a loaded disc culture system.

    PubMed

    Emanuel, K S; Vergroesen, P A; Peeters, M; Holewijn, R M; Kingma, I; Smit, T H

    2015-01-01

    The intervertebral disc (IVD) allows flexibility to the vertebral column, and transfers the predominant axial loads during daily activities. Its axial biomechanical behaviour is poroelastic, due to the water-binding and releasing capacity of the nucleus pulposus. Degeneration of the intervertebral disc presumably affects both the instantaneous elastic response to the load on the IVD and the subsequent interstitial flow of fluid. This study aims to quantify the poroelastic behaviour of the IVD and its change with degeneration, as defined by the magnetic resonance imaging-based Pfirrmann Score (PS). For a period of ten days, 36 human lumbar IVDs were loaded with a simulated physiological axial loading regime, while deformation was monitored. The IVDs responded to the loads with instantaneous elastic and slow poroelastic axial deformation. Several mechanical parameters changed throughout the first five days of the experiment, until the IVDs settled into a dynamic equilibrium. In this equilibrium, degeneration was significantly related to a decrease in disc height loss during the daytime high load phase (? = -0.49), and to a decrease in the rate of this deformation during the final half hour of each day (? = -0.53). These properties were related to the nucleus glycosaminoglycan/hydroxyproline (GAG/HYP) ratio, rather than GAG content alone, indicating that remodelling of the extracellular matrix reduces poroelastic properties of the IVD. This implies that the degenerated discs have a reduced capacity to bind water and/or a reduced resistance against fluid flow. The resulting loss in hydrostatic pressure may further change cell behaviour in the nucleus pulposus. PMID:26091731

  1. Secretion of soluble HLA-G by day 3 human embryos associated with higher pregnancy and implantation rates: assay of culture media using a new ELISA kit

    Microsoft Academic Search

    Nina Desai; Jessica Filipovits; James Goldfarb

    2006-01-01

    Identification of chemical markers in human embryo culture media that relate to embryo quality and implantation potential could be an invaluable tool in embryo selection for transfer. Embryonic secretion of soluble human leukocyte antigen G (sHLA-G) has been postulated to be a marker for ‘embryonic competence'. This study examines sHLA-G concentrations in day 3 culture media droplets of embryos that

  2. African Easterly Waves in 30-day High-Resolution Global Simulations: A Case Study During the 2006 NAMMA Period

    NASA Technical Reports Server (NTRS)

    Shen, Bo-Wen; Tao, Wei-Kuo; Wu, Man-Li C.

    2010-01-01

    In this study, extended -range (30 -day) high-resolution simulations with the NASA global mesoscale model are conducted to simulate the initiation and propagation of six consecutive African easterly waves (AEWs) from late August to September 2006 and their association with hurricane formation. It is shown that the statistical characteristics of individual AEWs are realistically simulated with larger errors in the 5th and 6th AEWs. Remarkable simulations of a mean African easterly jet (AEJ) are also obtained. Nine additional 30 -day experiments suggest that although land surface processes might contribute to the predictability of the AEJ and AEWs, the initiation and detailed evolution of AEWs still depend on the accurate representation of dynamic and land surface initial conditions and their time -varying nonlinear interactions. Of interest is the potential to extend the lead time for predicting hurricane formation (e.g., a lead time of up to 22 days) as the 4th AEW is realistically simulated.

  3. Two-day period fluctuation of PMC occurrence over Syowa Station, Antarctica observed by a ground-based lidar and AIM satellite.

    NASA Astrophysics Data System (ADS)

    Nakamura, T.; Suzuki, H.; Tsutsumi, M.; Ejiri, M. K.; Tomikawa, Y.; Abo, M.; Kawahara, T.; Tsuda, T. T.; Nishiyama, T.

    2014-12-01

    A Rayleigh/Raman lidar system has been operated by the Japanese Antarctic Research Expedition (JARE) since February, 2011 (JARE 52nd) in Syowa Station Antarctica (69.0S, 39.5E). The lidar system consists of a pulsed Nd:YAG laser (355nm) as a transmitter and two telescopes with four photo multiplier tubes which are to detect Rayleigh scattered light from low and high atmosphere at 355 nm and N2 Raman emission at 387nm. Polar Mesospheric Cloud (PMC) was detected by the lidar at 22:30UT (+3hr for LT) on Feb 4th, 2011, the first day of a routine operation. This event was the first time to detect PMC over Syowa Station by a lidar [Suzuki et al., Ann. Geophys., 2013]. However, signal to noise ratio (SNR) of the PMC event was not so good due to large shot noises from daytime background signals. Moreover, a receiver system was designed mainly for nighttime observations. In this way, observation of PMC during the midnight sun, which also corresponds to most frequent PMC season, was difficult. Thus, to improve SNR of the PMC observation with the lidar during daytime, a narrow band-pass Fabry-Perot etalon unit has been developed and installed in the receiver system on Dec 2013 by JARE 55th. By using this new system, clear PMC signals were successfully detected under daylight condition during the period of summer operation of JARE55th. During this period of 53 days (from 17 Dec. 2013 to 7 Feb. 2014), only 11 days were with a clear sky and suitable for PMC observation. Thus, it was difficult to study temporal variations on a PMC activity only by using the lidar data. Fortunately, NASA's AIM satellite had passed near Syowa Station and provided with complimentary PMC data during observation gap of the lidar. By combining our lidar data with the AIM/CIPS data, nearly continuous monitoring of PMC variability over Syowa Station was achieved for period between 13th and 18th in January 2014. PMC occurrence with an interval of two days over Syowa Station during the period was clearly confirmed. Co-located MF radar also showed clear two days fluctuation in horizontal wind velocities around PMC altitude during the same period. In this presentation, we will discuss the cause of the two-day oscillation found in PMC occurrence and horizontal wind velocity. In particular, two-day planetary wave will be quantitatively investigated as a potential cause of the fluctuation.

  4. Long-period (30 days1 year) electromagnetic sounding and the electrical conductivity of the lower mantle beneath Europe

    Microsoft Academic Search

    Nils Olsen

    1999-01-01

    The C-response connects the magnetic vertical component and the horizontal gradient of the horizontal components of electromagnetic variations and forms the basis for deriving the conductivity-depth profile of the Earth. Time-series of daily mean values at 42 observatories typically with 50 years of data are used to estimate C-responses for periods between 1 month and 1 yr. The Z: Y

  5. Long-period (30 days^1year) electromagnetic sounding and the electrical conductivity of the lower mantle beneath Europe

    Microsoft Academic Search

    Nils Olsen

    1999-01-01

    SUMMARY The C-response connects the magnetic vertical component and the horizontal gradient of the horizontal components of electromagnetic variations and forms the basis for deriving the conductivity^depth pro¢le of the Earth.Time-series of daily mean values at 42 observatories typically with 50 years of data are used to estimate C-responses for periods between 1 month and 1 yr. The Z:Y method

  6. Modulation of innate immune function and phenotype in bred dairy heifers during the periparturient period induced by feeding an immunostimulant for 60 days prior to delivery.

    PubMed

    Nace, E L; Nickerson, S C; Kautz, F M; Breidling, S; Wochele, D; Ely, L O; Hurley, D J

    2014-10-15

    The purpose of this study was to evaluate the effect of a feed additive (OmniGen-AF(®), reported to have immune modulating activity) on innate immunity and health events during the periparturient period in dairy heifers when immunity is suppressed. From 60 days prepartum through calving, supplemented heifers (n=20) received OmniGen-AF(®) daily and were compared with unsupplemented controls (n=20). Blood leukocyte innate immune activity (phenotype markers, phagocytic activity, and reactive oxygen species--ROS production) was measured prior to feeding (60 days prepartum), 30 days later, and on days 1, 7, 14, and 30 postpartum. Adverse health events (udder edema, ketosis, displaced abomasum, and death) and milk production were measured at calving and into early lactation. The fraction of leukocytes with measurable CD62L (L-selectin) on their surface from supplemented heifers tended to be greater during the periparturient period in treated heifers than controls (p=0.100). Likewise, leukocyte phagocytosis of Escherichia coli and Staphylococcus aureus during this time period tended to be greater in heifers supplemented with OmniGen-AF(®) (p=0.100). Conversely, ROS production in response to phorbol myristate acetate or when leukocytes were stimulated with killed S. aureus lysate tended to be greater among control heifers compared with supplemented animals (p=0.100). Supplemented heifers exhibited fewer incidents of udder edema than controls (p=0.030) and tended to exhibit a lower rate of new cases of mastitis (p=0.098); however, no differences were observed in milk somatic cell counts or level of milk production. Results demonstrate a positive role of OmniGen-AF(®) in amplifying leukocyte function consistent with antibacterial activity during the periparturient period, and support the continued study of dietary supplementation to enhance mammary gland health in dairy cows. PMID:25219783

  7. Montessori Language and the Sensitive Period for the Imagination and Culture.

    ERIC Educational Resources Information Center

    McKenzie, Ginger Kelley

    1995-01-01

    Proposes ways to create a language curriculum based on children's "sensitive periods" as described by Montessori. Suggests that ages 6 through 12 are a sensitive period for using imagination. Creative expression should be an integral part of the entire curriculum, and creative expression can be stimulated through many sources of writing…

  8. Heritage Film Audiences 2.0: Period Film Audiences and Online Fan Cultures

    Microsoft Academic Search

    Claire Monk

    2011-01-01

    In an update to my monograph Heritage Film Audiences: Period Films and Contemporary Audiences in the UK (Edinburgh University Press, 2011), this paper explores currently evolving forms of online audience behaviour and participatory fan activity around contemporary period films. Its detailed focus is on the online reception, (re-)appropriation and remixing of key films originally released in the 1980s to 1990s

  9. Johannes and the seven dwarfs: Kepler detection of low-level day-timescale periodic photometric variations in white dwarfs

    E-print Network

    Maoz, Dan; McQuillan, Amy

    2014-01-01

    We make use of the high photometric precision of Kepler to search for periodic variations among 14 normal (DA- and DB-type, likely non-magnetic) hot white dwarfs (WDs) observed by the mission. In seven of the WDs, we detect periodic, ~2 hr to 10 d, variations, with semi-amplitudes of 40 to 2000 ppm, lower than ever seen in WDs. We consider various explanations for the observed variations: WD rotation combined with magnetic cool spots; rotation combined with magnetic dichroism; rotation combined with hot spots from an interstellar-medium accretion flow, channeled onto weak magnetic poles; transits by size ~50-200 km objects; relativistic beaming due to reflex motion caused by a cool companion WD; or reflection/re-radiation of the primary WD light by a brown-dwarf companion, or by a giant planet of about Jupiter radius, undergoing illumination phases as it orbits the WD. Each of these mechanisms could be behind some of the variable WDs, but could not be responsible for all seven variable cases. Alternatively, t...

  10. Randomized, observer-blind, split-face study to compare the irritation potential of 2 topical acne formulations over a 14-day treatment period.

    PubMed

    Ting, William

    2012-08-01

    This randomized, observer-blind, split-face study assessed the irritation potential and likelihood of continued use of clindamycin phosphate 1.2%--benzoyl peroxide (BPO) 2.5% gel or adapalene 0.1%--BPO 2.5% gel once daily over a 14-day treatment period in 21 participants (11 males; 10 females) with acne who were 18 years or older. Investigator clinical assessment (erythema and dryness) and self-assessment (dryness and burning/stinging) were performed at baseline and each study visit (days 1-14) using a 4-point scale (O = none; 3 = severe). Transepidermal water loss (TEWL) and corneometry measurements were performed at baseline and days 3, 5, 7, 9, 11, and 14. Lesions were counted at baseline and on day 14. Participant satisfaction questionnaires were completed on days 7 and 14. At the end of the study, investigators reported none or only mild erythema in 86% (18/21) of participants treated with clindamycin phosphate 1.2%--BPO 2.5% gel compared with 62% (13/21) of participants treated with adapalene 0.1%--BPO 2.5% gel. No severe erythema was reported with clindamycin phosphate 1.2%--BPO 2.5% gel. Adapalene 0.1%--BPO 2.5% gel was prematurely discontinued due to severe erythema in 1 participant on day 5 and a second participant on day 9. Additionally, 2 more participants reported severe erythema on day 14. Mean erythema scores were 0.9 (mean change from baseline, 0.7) with clindamycin phosphate 1.2%--BPO 2.5% gel and 1.4 (mean change from baseline, 1.3) with adapalene 0. 1%--BPO 2.5% gel on day 14 (P < .05 for days 6-14). Similar results were seen with dryness. Mean scores were 0.5 (mean change from baseline, 0.4) and 1.0 (mean change from baseline, 1.0), respectively (P < .05 for days 6-14). Self-assessment, TEWL, and corneometry results underscored the investigator clinical assessment. Participant preference and likelihood of continued usage was greater with clindamycin phosphate 1.2%--BPO 2.5% gel. Continued use and efficacy results for the treatment of acne were influenced by the potential of the product to cause irritation and the participant preferences. Irritation potential was more pronounced and severe with adapalene 0.1%--BPO 2.5% gel. Undoubtedly, as a result more participants preferred treatment with clindamycin phosphate 1.2%--BPO 2.5% gel and were more likely to continue to use the product. PMID:22988653

  11. Natural Variation in the Drosophila melanogaster Clock Gene Period Modulates Splicing of Its 3?-Terminal Intron and Mid-Day Siesta

    PubMed Central

    Low, Kwang Huei; Chen, Wen-Feng; Yildirim, Evrim; Edery, Isaac

    2012-01-01

    Drosophila melanogaster exhibits circadian (?24 hr) regulated morning and evening bouts of activity that are separated by a mid-day siesta. Increases in daily ambient temperature are accompanied by a progressively longer mid-day siesta and delayed evening activity. Presumably, this behavioral plasticity reflects an adaptive response that endows D. melanogaster with the ability to temporally optimize daily activity levels over a wide range of physiologically relevant temperatures. For example, the shift in activity towards the cooler nighttime hours on hot days might minimize the risks associated with exposure to mid-day heat, whereas on cold days activity is favored during the warmer daytime hours. These temperature-induced shifts in the distribution of daily activity are partly based on the thermal sensitive splicing of an intron found in the 3? untranslated region (UTR) of the circadian clock gene termed period (per). As temperature decreases, splicing of this 3?-terminal intron (termed dmpi8) is gradually increased, which is causally linked to a shorter mid-day siesta. Herein we identify several natural polymorphisms in the per 3? UTR from wild-caught populations of flies originating along the east coast of the United States. Two non-intronic closely spaced single nucleotide polymorphisms (SNPs) modulate dmpi8 splicing efficiency, with the least efficiently spliced version associated with a longer mid-day siesta, especially at lower temperatures. Although these SNPs modulate the splicing efficiency of dmpi8 they have little to no effect on its thermal responsiveness, consistent with the notion that the suboptimal 5? and 3? splice sites of the dmpi8 intron are the primary cis-acting elements mediating temperature regulation. Our results demonstrate that natural variations in the per gene can modulate the splicing efficiency of the dmpi8 intron and the daily distribution of activity, providing natural examples for the involvement of dmpi8 splicing in the thermal adaptation of behavioral programs in D. melanogaster. PMID:23152918

  12. Three Cases of West Nile Encephalitis over an Eight-Day Period at a Downtown Los Angeles Community Hospital

    PubMed Central

    Puchalski, Adam; Liu, Antonio K.; Williams, Byron

    2015-01-01

    Since its introduction in New York City in 1999, the virus has spread throughout the entire North American continent and continues to spread into Central and Latin America. Our report discusses the signs and symptoms, diagnostics, and treatment of West Nile disease. It is important to recognize the disease quickly and initiate appropriate treatment. We present three cases of West Nile encephalitis at White Memorial Medical Center in East Los Angeles that occurred over the span of eight days. All three patients live within four to six miles from the hospital and do not live or work in an environment favorable to mosquitoes including shallow bodies of standing water, abandoned tires, or mud ruts. All the patients were Hispanic. Physicians and other health care providers should consider West Nile infection in the differential diagnosis of causes of aseptic meningitis and encephalitis, obtain appropriate laboratory studies, and promptly report cases to public health authorities. State governments should establish abatement programs that will eliminate sources that allow for mosquito reproduction and harboring. The public needs to be given resources that educate them on what entails the disease caused by the West Nile virus, what the symptoms are, and, most importantly, what they can do to prevent themselves from becoming infected. PMID:26106493

  13. 75 FR 1656 - Draft Safety Culture Policy Statement: Request for Public Comments; Extension of Comment Period

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-12

    ...nrc.gov. For the Nuclear Regulatory Commission: Dated at Rockville, Maryland, this 5th day of January 2010. Roy P. Zimmerman, Director, Office of Enforcement. [FR Doc. 2010-335 Filed 1-11-10; 8:45 am] BILLING CODE...

  14. Post-main-sequence and POST red giant branch variables with pulsation periods less than one day

    NASA Astrophysics Data System (ADS)

    Eggen, Olin J.

    1994-06-01

    Post-main-sequence (mass 1 to 3 solar masses) and post-giant branch (0.5 to 1 solar mass) pulsators are discussed on the basis of four color and H beta light curves published elsewhere. The post-main-sequence variables, called ultrashort period cepheid (USPC) (delta Sct), pulsate in the fundamental and first harmonic modes of radial pulsation and, in many cases, in nonradial modes. The variables for which photometry allows accurate, luminosity estimates and are known to pulsate simultaneously in the fundamental and first harmonic or in the fundamental mode alone, define a PL relation (MV = -2.80 log P - 0.60, fundamental). It is notable that the slope of this relation is in the range of slopes found for classical cepheids. Accurate V photometry is lacking for many of the variables known as 'anomalous cepheids', but the available data divide them into low mass, pseudocepheids (BL Her and W Vir stars) and post-main-sequence USPC (delta Sct) variables. Four USPC in NGC 5053 and six in NGC 6466, for which accurate photometry is available, give remarkably consistent moduli of 16.06 +/- 0.05 and 15.98 +/- 0.08 mag, respectively, for the clusters, in which they are blue stragglers similar to SX Phe in Kapteyn's star group. The assumption that the four post-giant branch variables, called VSPC (RR Lyr), S Ari, SU Dra, and ST Leo in Kapteyn's star group and RR Lyr in the Groombridge 1830 group, are physical members of these groups and share their V-velocities, leads to a calibration of the photometry for the derivation of reddening, luminosity, and heavy element abundance of 45 field variables. The resulting reddenings are consistent with values obtained by other methods and the metallicities are consistent with the most accurately available spectroscopic determinations of delta S and of Ca II K. The luminosities of the bulk of the variables confirm Sandage's (1993) relation between MV and (Fe/H). Four or five of the field variables are probably binary, including BB Vir which Kinman & Carreta (1992) have independently noted as double. The PL relation for USPC (delta Sct) variables intersects the horizontal branch (HB) near P = 0.3d and at least two field very short period cepheid (VSPC) (RR Lyr) star, FW Lup (0.484 d) and ST Pic (0.486 d) may be first overtone pulsators of the USPC (delta Sct) variety. A dozen field VSPC (RR Lyr) stars populate a (Fe/H), MV relation with the same slope as the other stars but displaced 0.7 mag toward higher luminosities. The only cluster variable found to populate this diplaced relation is No. 9 in 47 Tuc, although ST Vir, which may be a member of the Arcturus group, should also be considered. The elevated luminosities are unlikely to be caused by either evolution or errors in the photometric indices. A possible source of these apparently young VSPC(RR Lyr) variables with halo metallicity is in second (or third) generation globular clusters formed during an episodic collapse of the galaxy that produced metal poor stars but in a dynamical situation that hastened the disruption of the clusters, currently formed, before the still older globular clusters, created under conditions that have kept them in a more disruptive free environment.

  15. Modernism En Vogue: Popular Periodicals and Their Engagement with Modernist Culture

    Microsoft Academic Search

    Natalie Kalich

    2012-01-01

    My project investigates commercial magazines from the 1920s, including, The Saturday Evening Post, Ladies' Home Journal, Vogue, and Vanity Fair to reveal the frequency with which modernist writers contributed to these periodicals and the extent to which editors of these magazines found modernist discourse marketable to larger audiences, thereby undermining the assumption that modernists only spoke to a coterie audience.

  16. An Analysis of the Multi-Cultural Awareness Shown in Popular American Children's Periodicals.

    ERIC Educational Resources Information Center

    Markes, Mary J.

    Designed to provide guidance to elementary or middle school library media specialists in the selection of periodicals that will support a school's multicultural goals and objectives, this study developed a checklist and used it to analyze the treatment given to children and adults of various racial and ethnic groups in 15 of the most popular…

  17. Residues of oxytetracycline in cultured juvenile blue shrimp, Penaeus stylirostris (Crustacea: Decapod), fed medicated feed for 14 days

    Microsoft Academic Search

    Leone L Mohney; Rodney R Williams; Thomas A Bell; Donald V Lightner

    1997-01-01

    Juvenile shrimp fed for 14 days on oxytetracycline (OTC) medicated feed with a potency of 1500 mg OTC kg?1 of feed reached a level of 3.3 ?g OTC g?1 of shrimp tail muscle within 4 days, as measured by the microbial agar plate diffusion assay method for oxytetracycline in tissues, approved by the Association of Official Analytical Chemists. The concentration

  18. Musical rhythms in heart period dynamics: a cross-cultural and interdisciplinary approach to cardiac rhythms.

    PubMed

    Bettermann, H; Amponsah, D; Cysarz, D; van Leeuwen, P

    1999-11-01

    The purpose of this study was to expand classic heart period analysis methods by techniques from ethnomusicology that explicitly take complex musical rhythm principles into consideration. The methods used are based on the theory of African music, the theory of symbolic dynamics, and combinatorial theory. Heart period tachograms from 192 24-h electrocardiograms of 96 healthy subjects were transformed into binary symbol sequences that were interpretable as elementary rhythmic (percussive) patterns, the time lines in African music. Using a hierarchical rhythm pattern scheme closely related to the Derler Rhythm Classification (from jazz theory), we calculated the predominance and stability of pattern classes. The results show that during sleep certain classes, specific to individuals, occurred in a cyclically recurrent manner and many times more often than expected. Simultaneously, other classes disappeared more or less completely. Moreover, the most frequent classes obviously originate from phase-locking processes in autonomic regulation (e.g., between respiratory and cardiac cycles). In conclusion, the new interdisciplinary method presented here demonstrates that heart period patterns, in particular those occurring during night sleep, can be interpreted as musical rhythms. This method may be of great potential use in music therapy research. PMID:10564129

  19. Outdoor Day-Care Centres--A Culturalization of Nature: How Do Children Relate to Nature as Educational Practice?

    ERIC Educational Resources Information Center

    Melhuus, E. Cathrine

    2012-01-01

    This article will discuss how children and adults experience a certain outdoor environment as part of an educational practice, through the activities the adults and children have. It will further discuss how these activities realize cultural values through the educators' and children's activities. In Norway the use of outdoor environments has…

  20. Dancing as an Aspect of Early Mormon (Church of Jesus Christ of Latter-Day Saints) and Utah Culture.

    ERIC Educational Resources Information Center

    Holbrook, Leona

    Dance has often been a significant part of a cultural heritage. Most of the religious organizations which were formed just after the United States achieved its independence, however, rejected many European religious customs, including dance. Despite this, the Mormon church not only allowed dance, but advocated and sponsored it. Dance was an…

  1. Standard Fusion Protocol 1. Grow myeloma cells few days prior fusion date. Myeloma cells culture should be maintained

    E-print Network

    -warmed DMEM/F12 and centrifuge for 5 min at 800 rpm remove all traces of fetal calf serum (FCS); Count cells remove mouse spleen, place into sterile 100-mm cell culture plate containing 10 ml of sterile serum free DMEM/F12. Holding the spleen by sterile forceps perform spleen perfusion with serum free DMEM/F12

  2. Meanings, expressions, and experiences of care of chronically mentally ill in a day treatment center using Leininger's culture care theory

    Microsoft Academic Search

    Tamara Bloom George

    1998-01-01

    The purpose of this qualitative study was to discover the care meanings, expressions, and lived experiences of the chronically mentally ill living in an urban community as a subculture. This study was conceptualized within Leiningers Theory of Culture Care Diversity and Universality with the use of the Sunrise Model to explicate worldview, social structure factors, and historical and environmental context

  3. [The motive characteristics and pattern of manifestation of Chinese medicinal herb during the period of "Cultural Revolution"].

    PubMed

    Zhang, Wei; Zhang, Rui-xian

    2009-01-01

    During the period of "Cultural Revolution", there started a movement of Chinese medicinal herb throughout the whole country. As a national movement meeting the needs of the time, not only had the guarantee of national policy, but also had the support of finance and the propaganda of People's Daily, PLA Daily and Red Flag Magazine. The movement had all kinds of different manifestations such as cooperative medical care, mobile medical team, research team, three tu (folk recipe, folk healer, folk medicine), four selves (self-planting, self-collection, self-making, self-use), exhibition of Chinese medicinal herb, compiling the handbook of Chinese medicinal herb etc. It had reference values to the current medical care system. PMID:19824359

  4. St. Patrick's Day Activity

    NSDL National Science Digital Library

    MissNguyen

    2012-02-13

    Utah State 4th Grade Social Studies Core Standard 4: Objective 1- Demonstrate cultural understanding, by showing appreciation for the uniqueness of other cultures, identifying the contributions of various cultures in Utah, and demontrating respect for cultural differences. ~In groups of 6-7 students, search the link provided on your specific topic (Kwanzaa, St. Patrick's Day, or Pioneer Day). KWANZAA: http://holidays.kaboose.com/kwanzaa-index.html ST. PATRICKS DAY: http://www.lilesnet.com/St_Patricks/stpaddy.htm PIONEER DAY: http://heritage.uen.org/ ~Each group member is to present a part of their holiday presentation to the class. You will receive ...

  5. Statistical analysis of test-day milk yields using random regression models for the comparison of feeding groups during the lactation period.

    PubMed

    Mielenz, Norbert; Spilke, Joachim; Krejcova, Hana; Schüler, Lutz

    2006-10-01

    Random regression models are widely used in the field of animal breeding for the genetic evaluation of daily milk yields from different test days. These models are capable of handling different environmental effects on the respective test day, and they describe the characteristics of the course of the lactation period by using suitable covariates with fixed and random regression coefficients. As the numerically expensive estimation of parameters is already part of advanced computer software, modifications of random regression models will considerably grow in importance for statistical evaluations of nutrition and behaviour experiments with animals. Random regression models belong to the large class of linear mixed models. Thus, when choosing a model, or more precisely, when selecting a suitable covariance structure of the random effects, the information criteria of Akaike and Schwarz can be used. In this study, the fitting of random regression models for a statistical analysis of a feeding experiment with dairy cows is illustrated under application of the program package SAS. For each of the feeding groups, lactation curves modelled by covariates with fixed regression coefficients are estimated simultaneously. With the help of the fixed regression coefficients, differences between the groups are estimated and then tested for significance. The covariance structure of the random and subject-specific effects and the serial correlation matrix are selected by using information criteria and by estimating correlations between repeated measurements. For the verification of the selected model and the alternative models, mean values and standard deviations estimated with ordinary least square residuals are used. PMID:17036744

  6. NPR: Day to Day

    NSDL National Science Digital Library

    Hosted by award-winning National Public Radio (NPR) correspondent Alex Chadwick, Day to Day is a way for regular NPR listeners to listen to smart news coverage during the middle of the day or during their lunch-hour. Fortunately, Day to Day is available online, complete with an archive dating back to January 2003. Produced in the NPR West office in Los Angeles, and includes a number of NPR regulars and contributors from the online publication Slate. Day to Day also features the helpful daily reports from the Minnesota Public Radio show, Marketplace, which is "an informative conversation about business and economic news". Other regular features on Day to Day include commentaries on recent music releases from independent music critic Christian Bordal and curious and novel exposes and reports on the "odd underbelly" of the City of Angels, direct from Southern California.

  7. Valepotriate content in different in vitro cultures of Valerianaceae and characterization of Valeriana officinalis L. callus during a growth period

    Microsoft Academic Search

    C. Violon; N. van Cauwenbergh; A. Vercruysse

    1983-01-01

    Differentin vitro cultures of Valerianaceae were analysed for valepotriate content [(iso)valtrate, acevaltrate, didrovaltrate] in a study on\\u000a properties of productionin vitro (plant species, growth conditions, differentiation level, valepotriate content of the medium after growth). Thein vitro cultures were: callus cultures ofValeriana officinalis L., Valerianella locusta L. andCentranthus ruber L.DC.; a suspension culture ofValeriana officinalis L. and a root organ culture

  8. Good day! You are cordially invited to the events happening as part of Asian Heritage Awareness Month. In order to spread knowledge and awareness of Asian cultures, we have

    E-print Network

    Memphis, University of

    Good day! You are cordially invited to the events happening as part of Asian Heritage Awareness Month. In order to spread knowledge and awareness of Asian cultures, we have put together a month full culturally insightful comedy about ridiculous stereotypes, and share his experience growing as a regular

  9. 17 CFR 274.127f-1 - Form N-27F-1, notice to periodic payment plan certificate holders of 45-day withdrawal right with...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...false Form N-27F-1, notice to periodic payment plan certificate holders...withdrawal right with respect to periodic payment plan certificates. 274...127f-1 Form N-27F-1, notice to periodic payment plan certificate...

  10. 17 CFR 274.127f-1 - Form N-27F-1, notice to periodic payment plan certificate holders of 45-day withdrawal right with...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...false Form N-27F-1, notice to periodic payment plan certificate holders...withdrawal right with respect to periodic payment plan certificates. 274...127f-1 Form N-27F-1, notice to periodic payment plan certificate...

  11. 17 CFR 274.127f-1 - Form N-27F-1, notice to periodic payment plan certificate holders of 45-day withdrawal right with...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...false Form N-27F-1, notice to periodic payment plan certificate holders...withdrawal right with respect to periodic payment plan certificates. 274...127f-1 Form N-27F-1, notice to periodic payment plan certificate...

  12. 17 CFR 274.127f-1 - Form N-27F-1, notice to periodic payment plan certificate holders of 45-day withdrawal right with...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...false Form N-27F-1, notice to periodic payment plan certificate holders...withdrawal right with respect to periodic payment plan certificates. 274...127f-1 Form N-27F-1, notice to periodic payment plan certificate...

  13. 17 CFR 274.127f-1 - Form N-27F-1, notice to periodic payment plan certificate holders of 45-day withdrawal right with...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...false Form N-27F-1, notice to periodic payment plan certificate holders...withdrawal right with respect to periodic payment plan certificates. 274...127f-1 Form N-27F-1, notice to periodic payment plan certificate...

  14. A Whole-Genome Microarray Study of Arabidopsis thaliana Semisolid Callus Cultures Exposed to Microgravity and Nonmicrogravity Related Spaceflight Conditions for 5 Days on Board of Shenzhou 8

    PubMed Central

    Neef, Maren; Ecke, Margret; Hampp, Rüdiger

    2015-01-01

    The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1?g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production. PMID:25654111

  15. A whole-genome microarray study of Arabidopsis thaliana semisolid callus cultures exposed to microgravity and nonmicrogravity related spaceflight conditions for 5 days on board of Shenzhou 8.

    PubMed

    Fengler, Svenja; Spirer, Ina; Neef, Maren; Ecke, Margret; Nieselt, Kay; Hampp, Rüdiger

    2015-01-01

    The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1?g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production. PMID:25654111

  16. 41 CFR 302-6.107 - What effect do partial days of temporary quarters occupancy have on my authorized period for...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...Contracts and Property Management 4 2010-07-01 2010-07-01 false What effect do partial days of temporary...Contracts and Property Management Federal Travel Regulation... § 302-6.107 What effect do partial days of...

  17. The impact of performing bacterial identification and antimicrobial susceptibility testing on bronchoalveolar fluid cultures 24 h a day in a microbiology laboratory.

    PubMed

    Pailhoriès, Hélène; Lemarié, Carole; Kouatchet, Achille; Lasocki, Sigismond; Sargentini, Cyril; Kempf, Marie; Coron, Noémie; Mahaza, Chetaou; Joly-Guillou, Marie-laure; Eveillard, Matthieu

    2014-11-01

    We previously demonstrated the positive impact of performing bacterial identification and antimicrobial susceptibility testing (AST) after day hours (night service [NS]) for certain clinical samples on the treatment of infected patients. Our objective was to evaluate the impact of including positive bronchoalveolar lavage (BAL) cultures in our NS. Two major positive consequences were recorded: initiation of earlier appropriate treatment and earlier change to a reduced-spectrum but still effective regimen. Reductions in delay were defined as the differences between the hours actually spent and hours estimated as though laboratory tests had been performed in the absence of NS. Fifty BALs were included. The NS led to the implementation of earlier appropriate therapy in 10 cases (20%), to earlier de-escalation in 15 cases (30%), and to earlier appropriate therapy and de-escalation in 4 cases (8%). In conclusion, performing bacterial identification and AST for positive BAL after laboratory opening hours could be relevant. PMID:25149417

  18. Day to day with COPD

    MedlinePLUS

    ... but there are things you can do every day to keep COPD from getting worse, to protect ... COPD - day to day; Chronic obstructive airways disease - day to day; Chronic obstructive lung disease - day to day ; Chronic ...

  19. Remarks on mixotrophic and autotrophic carbon nutrition of Vitis plantlets cultured in vitro

    Microsoft Academic Search

    Rose Galzy; Daniel Compan

    1992-01-01

    Two Vitis species were cultured in vitro under photoautrophic (sucrose-free culture medium) and photomixotrophic (sucrose 15 g l-1) conditions during the period following microcutting rooting (day 34 to day 120). Several parameters were measured at the end of the culture: growth, plant dry weight, carbohydrate uptake from the medium and rates of photosynthesis and dark respiration. The two species behaved

  20. KELT-6b: A P ? 7.9 day hot Saturn transiting a metal-poor star with a long-period companion

    SciTech Connect

    Collins, Karen A.; Kielkopf, John F. [Department of Physics and Astronomy, University of Louisville, Louisville, KY 40292 (United States); Eastman, Jason D. [Las Cumbres Observatory Global Telescope Network, 6740 Cortona Drive, Suite 102, Santa Barbara, CA 93117 (United States); Beatty, Thomas G.; Gaudi, B. Scott [Department of Astronomy, The Ohio State University, 140 West 18th Avenue, Columbus, OH 43210 (United States); Siverd, Robert J.; Pepper, Joshua; Stassun, Keivan G. [Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37235 (United States); Johnson, John Asher [Department of Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Howard, Andrew W.; Fulton, Benjamin J. [Institute for Astronomy, University of Hawaii, 2680 Woodlawn Drive, Honolulu, HI 96822 (United States); Fischer, Debra A. [Department of Astronomy, Yale University, New Haven, CT 06511 (United States); Manner, Mark [Spot Observatory, Nunnelly, TN 37137 (United States); Bieryla, Allyson; Latham, David W. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Gregorio, Joao [Atalaia Group and Crow-Observatory, Portalegre (Portugal); Buchhave, Lars A. [Niels Bohr Institute, University of Copenhagen, Juliane Maries vej 30, DK-21S00 Copenhagen (Denmark); Jensen, Eric L. N. [Department of Physics and Astronomy, Swarthmore College, Swarthmore, PA 19081 (United States); Penev, Kaloyan [Princeton University, Princeton, NJ 08544 (United States); Crepp, Justin R. [Department of Physics, University of Notre Dame, 225 Nieuwland Science Hall, Notre Dame, IN 46556 (United States); and others

    2014-02-01

    We report the discovery of KELT-6b, a mildly inflated Saturn-mass planet transiting a metal-poor host. The initial transit signal was identified in KELT-North survey data, and the planetary nature of the occulter was established using a combination of follow-up photometry, high-resolution imaging, high-resolution spectroscopy, and precise radial velocity measurements. The fiducial model from a global analysis including constraints from isochrones indicates that the V = 10.38 host star (BD+31 2447) is a mildly evolved, late-F star with T {sub eff} = 6102 ± 43 K, log?g{sub ?}=4.07{sub ?0.07}{sup +0.04}, and [Fe/H] = –0.28 ± 0.04, with an inferred mass M {sub *} = 1.09 ± 0.04 M {sub ?} and radius R{sub ?}=1.58{sub ?0.09}{sup +0.16} R{sub ?}. The planetary companion has mass M{sub P} = 0.43 ± 0.05 M {sub Jup}, radius R{sub P}=1.19{sub ?0.08}{sup +0.13} R{sub Jup}, surface gravity log?g{sub P}=2.86{sub ?0.08}{sup +0.06}, and density ?{sub P}=0.31{sub ?0.08}{sup +0.07} g cm{sup ?3}. The planet is on an orbit with semimajor axis a = 0.079 ± 0.001 AU and eccentricity e=0.22{sub ?0.10}{sup +0.12}, which is roughly consistent with circular, and has ephemeris of T {sub c}(BJD{sub TDB}) = 2456347.79679 ± 0.00036 and P = 7.845631 ± 0.000046 days. Equally plausible fits that employ empirical constraints on the host-star parameters rather than isochrones yield a larger planet mass and radius by ?4)-7). KELT-6b has surface gravity and incident flux similar to HD 209458b, but orbits a host that is more metal poor than HD 209458 by ?0.3 dex. Thus, the KELT-6 system offers an opportunity to perform a comparative measurement of two similar planets in similar environments around stars of very different metallicities. The precise radial velocity data also reveal an acceleration indicative of a longer-period third body in the system, although the companion is not detected in Keck adaptive optics images.

  1. mean live weight of 32 kg were kept in individual pens and received only cereal based diets (97 p. 100 oats). They were subjected to a 10-days faeces collection period. Three types

    E-print Network

    Paris-Sud XI, Université de

    . 100 oats). They were subjected to a 10-days faeces collection period. Three types of oats were tested : two regular oats (black and white) and one batch of naked oats containing 15.0, 11.8 and 2.9 p. 100 relationships : The results showed that the energy value (DE) of oats was closely related to the cell wall

  2. Parathyroid hormone blocks the stimulatory effect of insulin-like growth factor-I on collagen synthesis in cultured 21-day fetal rat calvariae

    SciTech Connect

    Kream, B.E.; Petersen, D.N.; Raisz, L.G. (Univ. of Connecticut Health Center, Farmington (USA))

    1990-01-01

    We examined the interaction of parathyroid hormone (PTH) and recombinant human insulin-like growth factor I (IGF-I) on collagen synthesis in 21-day fetal rat calvariae as assessed by measuring the incorporation of ({sup 3}H)proline into collagenase-digestible protein. After 96 hours of culture, 10 nM PTH antagonized the stimulation of collagen synthesis and partially blocked the increase in dry weight produced by 10 nM IGF-I. The effect of PTH to block IGF-I stimulated collagen synthesis was observed in the central bone of calvariae and was mimicked by forskolin and phorbol 12-myristate 13-acetate, but not by 1,25-dihydroxyvitamin D3, transforming growth factor-alpha or dexamethasone. Our data are consistent with the concept that the direct effect of PTH is to inhibit basal CDP labeling and fully oppose IGF-I stimulated CDP labeling. The finding that this effect of PTH is mimicked by forskolin and PMA suggests that this block in IGF-I stimulation of CDP labeling involves both cAMP and protein kinase C mediated pathways.

  3. LEMERCIER O. (sous presse) Campaniformes et cultures locales en France mditerranenne, in : TUREK J., KRUTOVA M. (Eds) : Beaker Days I, Prague, sous presse.

    E-print Network

    Boyer, Edmond

    of the final Neolithic in this area. The phase of synchrony between Bell Beakers and local cultures of composite culture and geographical exclusions. Keywords : Prehistory ; Neolithic ; Final Neolithic ; Bell

  4. Producing biodiesel from cotton seed oil using Rhizopus oryzae ATTC #34612 whole cell biocatalysts: Culture media and cultivation period optimization

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effect of culture medium composition and cultivation time on biodiesel production by Rhizopus oryzae ATCC #34612 whole cell catalysts, immobilized on novel rigid polyethylene biomass supports, was investigated. Supplementation of the medium with carbon sources led to higher lipase activity and i...

  5. Influence of algae species, substrata and culture conditions on attached microalgal culture.

    PubMed

    Shen, Y; Xu, X; Zhao, Y; Lin, X

    2014-03-01

    The objective of this study was to understand and optimize the formation of microalgae biofilms in specific culture conditions. Firstly, the adhesion of six freshwater algae species was compared. Chlorococcum sp. was selected because of the high adhesion biomass productivity (ABP) and adhesion rate achieved. Secondly, the adhesion of Chlorococcum sp. was compared with nine commonly used supporting materials, and glass fiber-reinforced plastic proved to be the optimal substrata. Thirdly, based on response surface methodology experiments, a second-order polynomial model was developed to examine the effect of culture period, initial total nitrogen concentration (ITNC) in manure wastewater, pH and culture volume of the growth chamber on the adhesion of Chlorococcum sp. using glass fiber-reinforced plastic. The experimental and modeling results showed that ITNC, pH and culture volume as well as the interactions between culture period and ITNC, culture period and culture volume were significant on ABP. Optimum culture conditions were predicted at a culture period of 11 days, ITNC of 70 mg L(-1), pH of 8 and culture volume of 340 mL, under which the predicted maximum ABP was 4.26 g m(-2) day(-1). The prediction was close to validation experimental results, indicating that the model could be used to guide and optimize the attached culture of Chlorococcum sp. using glass fiber-reinforced plastic. PMID:23843094

  6. A global climate-chemistry model study of present-day tropospheric chemistry and radiative forcing from changes in tropospheric O3 since the preindustrial period

    Microsoft Academic Search

    Sun Wong; Wei-Chyung Wang; Ivar S. A. Isaksen; Terje K. Berntsen; Jostein K. Sundet

    2004-01-01

    We present simulations of present-day tropospheric O3 and of changes in its concentrations and the associated radiative forcing since the industrial revolution. Numerical experiments were conducted using a global tropospheric climate-chemistry model (GCCM) developed by incorporating the University of Oslo (UiO) photochemical module (a reduced tracer scheme that lumps nonmethane volatile organic compounds (NMVOCs) to simulate the CO-NOx-HOx-O3 system) into

  7. Clonally Derived Human Embryonic Stem Cell Lines Maintain Pluripotency and Proliferative Potential for Prolonged Periods of Culture

    Microsoft Academic Search

    Michal Amit; Melissa K. Carpenter; Margaret S. Inokuma; Choy-Pik Chiu; Charles P. Harris; Michelle A. Waknitz; Joseph Itskovitz-Eldor; James A. Thomson

    2000-01-01

    Embryonic stem (ES) cell lines derived from human blastocysts have the developmental potential to form derivatives of all three embryonic germ layers even after prolonged culture. Here we describe the clonal derivation of two human ES cell lines, H9.1 and H9.2. At the time of the clonal derivation of the H9.1 and H9.2 ES cell lines, the parental ES cell

  8. Less incidence of coronary artery disease in general anesthesia compared to spinal-epidural anesthesia after total knee replacement: 90-day follow-up period by a population-based dataset.

    PubMed

    Hsieh, Jui-Yang; Lin, Hui-Wen

    2015-07-01

    Total knee replacement (TKR) is an effective and safe procedure. However, large-scale study to compare the incidence of coronary artery disease (CAD) after spinal or epidural anesthesia (SA-EA) or general anesthesia (GA) for TKR has not ever been conducted. To do so, we studied a population-based dataset from the Taiwan National Health Research Institute and hypothesized that the incidence of CAD might be different with regional than with general anesthesia. The risk of CAD-related events during a 90-day follow-up period among patients who received TKR under SA-EA or GA was evaluated in the present study. A total of 1500 patients from the Taiwan National Health Insurance claims database who underwent TKR from January 1, 2004, to December 31, 2006, were allocated into two groups. Group 1 included 1012 patients who received SA-EA during TKR procedure. Group 2 included 488 patients who received GA during this procedure. The number of patients who developed CAD during the 90-day follow-up period was 31 (3.1 %) in group 1 and 6 (1.2 %) in group 2. The Kaplan-Meier survival analysis of IHD-free cumulative survival rate during the 90-day follow-up period for patients who underwent TKR was significantly lower in group 1 than in group 2. The hazard ratio for the occurrence of CAD was 2.80 (95 % CI 1.16-6.78), and the hazard was higher for patients who received SA-EA than for patients who received GA after adjusted potential confounding factors. After the performance of TKR, patients had a potentially increased risk for CAD in SA-EA compared to GA during the 90-day follow-up period. PMID:25761988

  9. Excess of mortality in patients with chest pain peaks in the first 3 days period after the incident and normalizes after 1 month

    PubMed Central

    Bruyninckx, Rudi; Van den Bruel, Ann; Buntinx, Frank; Van Casteren, Viviane; Aertgeerts, Bert

    2010-01-01

    Background. Patients presenting with chest pain have a 5% chance of experiencing a coronary event. These patients are at risk of mortality and should be recognized and referred to secondary care. Aim. To determine the relationship between referral type and mortality in patients with chest pain. Methods. The design of the study is an observational study. The setting of the study is a sentinel network of general practices in Belgium, covering 1.6% of the total population. The subjects are 1558 consecutive patients consulting with chest pain in 2003. Descriptive analyses report the standardized mortality ratios. We used the Belgian population of 1999 as the reference population and as the standard population. Results. The standardized mortality ratios of 3 days were 151.0 [95% confidence interval (CI): 82.3–250.3] for the urgent referred group, 45.5 (95% CI: 12.4–116.0) for non-urgent and 13.6 (95% CI: 1.7–49.4) for the non-referred group. The standardized ratios of 1 month were, respectively, 27.6 (95% CI: 18.0–40.4), 6.7 (95% CI: 2.5–14.6) and 4.7 (95% CI: 1.9–9.7). The standardized ratios of 2–12 months were normal for the urgent referral group (1.3; 95% CI: 0.7–2.2) and for the non-urgent referral group (1.0; 95% CI: 0.5–1.9) and even less in the non-referred group (0.4; 95% CI: 0.2–0.9). Conclusions. Mortality in the first 3 days and first month after consulting for chest pain is very high. There is a marked trend in mortality according to the referral type—urgently referred, non-urgently referred and not referred—suggesting risk stratification by the GP. After 1 month, mortality normalizes for all groups, suggesting that the surviving patients are well treated and the condition causing the chest pain no longer influences survival compared to the general population. PMID:20639281

  10. Observations on the flight paths of the day-flying moth Virbia lamae during periods of mate location: do males have a strategy for contacting the pheromone plume?

    PubMed

    Cardé, Ring T; Cardé, Anja M; Girling, Robbie D

    2012-01-01

    1. To maximize the probability of rapid contact with a female's pheromone plume, the trajectories of male foraging flights might be expected to be directed with respect to wind flow and also to be energetically efficient. 2. Flights directed either upwind, downwind, or crosswind have been proposed as optimal strategies for rapid and/or energetically efficient plume contact. Other possible strategies are random and Lévy walks, which have trajectories and turn frequencies that are not dictated by the direction of wind flow. 3. The planar flight paths of males of the day-active moth Virbia lamae were recorded during the customary time of its sexual activity. 4. We found no directional preference in these foraging flights with respect to the direction of contemporaneous wind flow, but, because crosswind encompasses twice the possible orientations of either upwind or downwind, a random orientation is in effect a de facto crosswind strategy. 5. A crosswind preference should be favoured when the plume extends farther downwind than crosswind, and this strategy is realized by V. lamae males by a random orientation of their trajectories with respect to current wind direction. PMID:21729068

  11. Japan: The Modernization of an Ancient Culture. Series on Public Issues No. 3.

    ERIC Educational Resources Information Center

    Wolken, Lawrence C.

    This booklet, one of a series of booklets intended to apply economic principles to major social and political issues of the day, traces the modernization of the ancient culture of Japan. Four major areas are covered: (1) "An Ancient Culture" covers the period from the first settling of Japan through the Heian period, the medieval ages, the Meiji…

  12. "I Go to School Six Days a Week": The Role of Cultural and Religious Practices within Hybrid Turkish-American Communities in Supporting Academic and Socioemotional Growth

    ERIC Educational Resources Information Center

    Isik-Ercan, Zeynep

    2012-01-01

    Children's participation in cultural activities within the community, and the particular cognitive and socioemotional skills that they gain as a result of their participation, have been thoroughly studied (Cole, 1990; Gallimore & Tharp, 1990; Moll et al., 2005; Scribner & Cole, 1981). However, the connection of these skills to school learning or…

  13. Earth Day

    NSDL National Science Digital Library

    On April 22, 2005, people around the world will celebrate the 35th anniversary of Earth Day. This Topic in Depth focuses on the past and present of this significant day. From the Wisconsin Historical Society, the first two sites contain historical documents pertaining to Earth Day. The first (1) document features a May 1970 issue of The Gaylord Nelson Newsletter reporting on the first Earth Day. The second (2) document is a speech by Nelson entitled "An Environmental Agenda for the 70's." Housed in the archives of the US Environmental Protection Agency (EPA) website, the next two sites also contain historical documents. The first (3) site contains an article written by Nelson for the EPA Journal in April of 1980, entitled "Earth Day '70: What It Meant." The second (4) site contains an article written by John C. Whitaker (former Interior undersecretary in the Nixon administration) for the EPA Journal in the summer of 1998. The article is entitled "Earth Day Recollections: What It Was Like When the Movement Took Off." The (5) Earth Day Network (first mentioned in the April 4, 2003, Scout Report for Life Sciences) works "to broaden the environmental movement worldwide and to educate and mobilize people, governments, and corporations to take responsibility for a clean and healthy environment." In addition to information sections about Ongoing Programs, Current Campaigns, and News, the Earth Day Network website contains Earth Day 2005 Materials for organizers. From EarthDay.gov, Take Action In Your Classroom (6) offers links to a variety of environmental education resources. The next website, from the U.S. Army Environmental Center, presents (7) Army Earth Day; and links to information about the Army's environmental activities. The final (8) site is an Earth Day-inspired educational website (first reported on in the April 14, 1999 Scout Report for Science & Engineering) from the Wilderness Society. The site offers a collection of environmental education resources for teachers and students. [NL

  14. Space Day

    NSDL National Science Digital Library

    Space Day '98, to be held on May 21, 1998, celebrates and honors the people who have made space exploration possible. The highlight of the Space Day homepage, maintained by Lockheed Martin Corporation, will be a live interactive webcast all day on May 21. Features of the webcast include experts in the fields of space discussing issues ranging from global collaboration to mysteries that remain. In addition to the webcast, the Space Day '98 home page provides resources for teachers and the curious alike. Mission: Fun allows visitors to test their space knowledge through interactive Shockwave games and quizzes while Teachers' Space provides educators with downloadable lesson plans (Adobe Acrobat [.pdf] format).

  15. Dinosaur Day!

    ERIC Educational Resources Information Center

    Nakamura, Sandra; Baptiste, H. Prentice

    2006-01-01

    In this article, the authors describe how they capitalized on their first-grade students' love of dinosaurs by hosting a fun-filled Dinosaur Day in their classroom. On Dinosaur Day, students rotated through four dinosaur-related learning stations that integrated science content with art, language arts, math, and history in a fun and time-efficient…

  16. The effect of magnetic field on Paulownia tissue cultures

    Microsoft Academic Search

    Orkun Yaycili; Sema Alikamanoglu

    2005-01-01

    In this study, in vitro tissue cultures of Paulownia tomentosa and Paulownia fortunei were prepared and then exposed to a magnetic flow density of 2.9–4.8 mT and 1 m s?1 flow rate for a period of 0, 2.2, 6.6 and 19.8 s. The magnetic field (MF) increased the regeneration capability of Paulownia cultures and shortened the regeneration time. On the 28th day of culture,

  17. Enhancement of paclitaxel production by temperature shift in suspension culture of Taxus chinensis.

    PubMed

    Choi; Kim; Son; Hong; Lee; Lee

    2000-11-01

    Effect of temperature shift during culture period on cell growth and paclitaxel was investigated to optimize paclitaxel production in suspension culture of Taxus chinensis. Cell growth showed the optimum at 24 degrees C while paclitaxel synthesis showed the maximum at 29 degrees C. To minimize the inhibitory effect of higher temperature on cell growth, temperature was shifted after a certain period of culture time at 24 degrees C. Paclitaxel synthesis in plant cell culture increased dramatically during day 14 to day 21 regardless of treatment, reaching the maximum production of 137.5 mg paclitaxel/L. When the temperature was maintained at 29 degrees C after day 21, the specific productivity of paclitaxel was sustained for prolonged period of 42 days. The possible relationship between temperature and paclitaxel synthetic pathway was also suggested. PMID:11024522

  18. Three-dimensional resistivity tomography in extreme coastal terrain amidst dense cultural signals: application to cliff stability assessment at the historic D-Day site

    NASA Astrophysics Data System (ADS)

    Udphuay, Suwimon; Günther, Thomas; Everett, Mark E.; Warden, Robert R.; Briaud, Jean-Louis

    2011-04-01

    Pointe du Hoc overlooking the English Channel in Normandy, France was host to one of the most important military engagements of World War II but is vulnerable to cliff collapses that threaten important German fortifications including the forward observation post (OP) and Rudder's command post. The objective of this study is to apply advanced 3-D resistivity tomography towards a detailed site stability assessment with special attention to the two at-risk buildings. 3-D resistivity tomography data sets at Pointe du Hoc in the presence of extreme topography and dense cultural clutter have been successfully acquired, inverted and interpreted. A cliff stability hazard assessment scheme has been designed in which regions of high resistivity are interpreted as zones of open, dry fractures with a moderate mass movement potential. Regions of low resistivity are zones of wet, clay-filled fractures with a high mass movement potential. The OP tomography results indicate that the highest mass movement hazard appears to be associated with the marine caverns at the base of the cliff that are positioned at the point of strongest wave attack. These caverns likely occupy the future site of development of a sea arch that will threaten the OP building. The mass movement potential at the Rudder's command post area is low to moderate. The greatest risk there is associated with soil wedge failures at the top of the cliffs.

  19. Inspire Day

    ERIC Educational Resources Information Center

    Bohach, Barbara M.; Meade, Birgitta

    2014-01-01

    The authors collaborated on hosting a "Spring Inspire Day." planned and delivered by preservice elementary teachers as a social studies/science methods project. Projects that have authentic application opportunities can make learning meaningful for prospective teachers as well as elementary students. With the impetus for an integrated…

  20. Dinosaur Day!

    NSDL National Science Digital Library

    H. Prentice Baptiste

    2006-01-01

    On Dinosaur Day, first-grade students rotated through four dinosaur-related learning stations that integrated science content with art, language arts, math, and history in a fun and time-efficient manner. The event drew parents, teachers, and students together as they helped each other discuss, write, draw, measure, mix, and record at each learning station.

  1. Election Day

    NSDL National Science Digital Library

    University of Washington

    Election Day teaches how elections work in the United States. Players learn about political parties, public opinion, campaign financing, election law, political marketing, strategic planning, and the media. The game is well-suited for high-school and college students.

  2. Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system.

    PubMed

    Machen, Alexandra; Drake, Tim; Wang, Yun F Wayne

    2014-01-01

    Rapid identification and antimicrobial susceptibility testing of microorganisms causing bloodstream infections or sepsis have the potential to improve patient care. This proof-of-principle study evaluates the Lysis-Filtration Method for identification as well as antimicrobial susceptibility testing of bacteria directly from positive blood culture bottles in a clinical setting. A total of 100 non-duplicated positive blood cultures were tested and 1012 microorganism-antimicrobial combinations were assessed. An aliquot of non-charcoal blood culture broth was incubated with lysis buffer briefly before being filtered and washed. Microorganisms recovered from the filter membrane were first identified by using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight VITEK® Mass Spectrometry (VITEK MS). After quick identification from VITEK MS, filtered microorganisms were inoculated to VITEK®2 system for full panel antimicrobial susceptibility testing analysis. Of 100 bottles tested, the VITEK MS resulted in 94.0% correct organism identification to the species level. Compared to the conventional antimicrobial susceptibility testing methods, direct antimicrobial susceptibility testing from VITEK®2 resulted in 93.5% (946/1012) category agreement of antimicrobials tested, with 3.6% (36/1012) minor error, 1.7% (7/1012) major error, and 1.3% (13/1012) very major error of antimicrobials. The average time to identification and antimicrobial susceptibility testing was 11.4 hours by using the Lysis-Filtration method for both VITEK MS and VITEK®2 compared to 56.3 hours by using conventional methods (p<0.00001). Thus, the same-day results of microorganism identification and antimicrobial susceptibility testing directly from positive blood culture can be achieved and can be used for appropriate antibiotic therapy and antibiotic stewardship. PMID:24551067

  3. Enhanced recovery of Phytophthora ramorum from soil following 30 days storage at 4C

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Chlamydospores of Phytophthora ramorum produced by mixing 20 percent V8 juice broth cultures with sand and incubating over a 30 day period were used to infest field soil at densities ranging from 0.2 to 42 chlamydospores per cubic centimeter of soil. Chlamydospore recovery was determined by baiting...

  4. From Highbury to Hollywood and back again: Jane Austen's materialization in popular culture 

    E-print Network

    Heinemann, Rebecca Elizabeth

    2013-02-22

    -day commodity culture, about current notions of class and culture (especially "high" culture versus popular/"low" culture), and about how Jane Austen functions as an idea within modern-day notions of culture. Most people seem to understand culture...

  5. Hydrology day

    NASA Astrophysics Data System (ADS)

    Morel-Seytoux, H. J.

    Registration for the Hydrology Day sponsored by the Front Range Branch of AGU on April 23 at Colorado State University in Fort Collins, Colorado, totaled 121 participants, of whom 61 were students.Thirty-one individuals joined the Front Range Branch. Three students from Colorado State University won the awards for best paper in their category: Thomas W. Anzia (Sr.), ‘A Comprehensive Table of Standard Deviates for Confidence Limits on Extreme Events’ Victor Nazareth (M.S.), ‘Aquifer Properties from Single-Hole Aquifer Tests’ and Roy W. Koch (Ph.D.), ‘A Physically Based Derivation of the Distribution of Excess Precipitation.’ Judges for the awards were Dr. Bittinger, Resource Consultants, Fort Collins; George Leavesley and Daniel Bauer, USGS, Water Resources Division, Denver; Scott Tucker, Executive Director, Denver Urban Drainage and Flood Control District; Charles Brendecke, Department of Civil Engineering, Univ. of Colorado, Boulder.

  6. In vitro maintenance of spermatogenesis in Xenopus laevis testis explants cultured in serum-free media

    SciTech Connect

    Risley, M.S.; Miller, A.; Bumcrot, D.A.

    1987-05-01

    Spermatogenesis has been maintained for extended periods in Xenopus laevis testis explants cultured in serum-free media supplemented with bovine serum albumin, insulin, transferrin, follicle-stimulating hormone, dihydrotestosterone, testosterone, retinol, ascorbate, and tocopherol. The organization of the testis fragments was maintained for 28 days, and all stages of development were present throughout the culture period. /sup 3/H-Thymidine-labeled secondary (Type B) spermatogonia developed in 28 days into spermatids at the acrosomal vesicle stage whereas labeled zygotene spermatocytes became mature spermatids in 28 days. Spermatogonial proliferation also continued in vitro for 28 days. Germ cell differentiation was not dependent upon exogenous testosterone, ascorbate, or tocopherol since /sup 3/H-labeled spermatogonia became mature spermatids in testes cultured 35 days in media lacking these supplements. Autoradiography demonstrated that 55% of the luminal sperm present in explants cultured 10 days had differentiated in vitro. Sperm from testes cultured 10-35 days were similar to sperm from freshly dissected testes with regard to motility and fecundity, and eggs fertilized with sperm from explant cultures developed normally into swimming tadpoles. The results demonstrate the feasibility of maintaining vertebrate spermatogenesis in culture and suggest that in vitro analysis of Xenopus spermatogenesis using defined media may provide important insights into the evolution of regulatory mechanisms in spermatogenesis.

  7. Response of mixed-age cultures of phosphine-resistant and susceptible strains of lesser grain borer, Rhyzopertha dominica, to phosphine at a range of concentrations and exposure periods

    Microsoft Academic Search

    Patrick J. Collins; Gregory J. Daglish; Hervoika Pavic; Rosemary A. Kopittke

    2005-01-01

    Mixed-age cultures, containing all life stages, of a highly resistant strain (Strong-R) of lesser grain borer, Rhyzopertha dominica (F.), were exposed to a series of fixed concentrations of phosphine at a range of exposure periods at 25°C. A susceptible strain and a less-resistant strain (Weak-R) were also tested. The aim was to characterise the resistant strain and determine if it

  8. Laboratory Culture of Tautog

    Microsoft Academic Search

    Dean M. Perry; Renee Mercaldo-Allen; Catherine A. Kuropat; James B. Hughes

    1998-01-01

    Spawning of field-captured adult tautog Tautoga onitis was accomplished under laboratory conditions. Natural spawning of tautog produced more viable embryos and larvae than did strip-spawning. Embryos were cultured to hatching and raised successfully through the larval stage to juveniles. Newly hatched larvae were fed protozoans from day 0 to day 6 posthatch, rotifers from day 2 to day 20 posthatch,

  9. Effect of cultural conditions on the production of polygalacturonase by Rhizoctonia bataticola

    Microsoft Academic Search

    A. C. Amadioha

    2007-01-01

    Polygalacturonase (PG) activity was detected in culture filtrate and Rhizoctonia-infected tissues of potato tubers (Solanum tuberosum L.). Culture-produced PG formed more reducing groups than PG from diseased tissue. PG production and pH of the culture medium increased with the incubation period and attained optimal values between the 4th and 6th days. The ability of R. bataticola to produce PG varied

  10. Inhibitory Effects of Fetal Calf Serum on Proliferation of Chick Neuroblasts in Culture

    Microsoft Academic Search

    Ibtissam Barakat; Gérard Labourdette; Monique Sensenbrenner

    1983-01-01

    In this study, the proliferative activity of cultured chick neuroblasts exposed to high or low concentrations of fetal calf serum (FCS) was investigated. Different experimental evidences showed that the first 2–3 days of culture correspond to a period of neuronal proliferation in our culture conditions, whereas contamination by glial cells is very low. Cell counts and measurements of [3H]-thymidine incorporation

  11. Stennis Space Center celebrates Diversity Day

    NASA Technical Reports Server (NTRS)

    2009-01-01

    Kendall Mitchell of the Naval Oceanographic Office (right) learns about the culture of Bolivia from Narda Inchausty, president of the Foreign Born Wives Association in Slidell, La., during 2009 Diversity Day events at NASA's John Stennis Space Center. Stennis hosted Diversity Day activities for employees on Oct. 7. The day's events included cultural and agency exhibits, diversity-related performances, a trivia contest and a classic car and motorcycle show. It also featured the first-ever sitewide Stennis Employee Showcase.

  12. In vitro maintenance of spermatogenesis in Xenopus laevis testis explants cultured in serum-free media

    Microsoft Academic Search

    M. S. Risley; A. Miller; D. A. Bumcrot

    1987-01-01

    Spermatogenesis has been maintained for extended periods in Xenopus laevis testis explants cultured in serum-free media supplemented with bovine serum albumin, insulin, transferrin, follicle-stimulating hormone, dihydrotestosterone, testosterone, retinol, ascorbate, and tocopherol. The organization of the testis fragments was maintained for 28 days, and all stages of development were present throughout the culture period. ³H-Thymidine-labeled secondary (Type B) spermatogonia developed in

  13. Betelgeuse Period Analysis Using VSTAR

    NASA Astrophysics Data System (ADS)

    Dempsey, F.

    2015-06-01

    (Abstract only) Betelgeuse was studied using the VSTAR software package and analysis of the observations in the AAVSO database. Period analysis derived a period of 376 days, in comparison with literature periods of 420 days using satellite UV data but significantly different from the VSX period of 2,335 days. The unique set of PEP observations of this star is also shown and advantage of PEP Johnson V observations is shown in comparison with the visual observations.

  14. Change of address is an event-based SEVIS reporting requirement. Address verification is also required for initial SEVIS registration and periodic 30-day status reporting in the beginning of each fall

    E-print Network

    Fernandez, Eduardo

    Change of address is an event-based SEVIS reporting requirement. Address verification is also visa classification must report a change of address to the school within 10 days from occurrence. ISSS has 21 days from receiving the information to report the change of address to SEVIS. ISSS will use

  15. Pesticides Safely For more information on selection, planting, cultural

    E-print Network

    Liskiewicz, Maciej

    Applying Pesticides Safely For more information on selection, planting, cultural practices and instructions. Observe the days-to- harvest period for fruits and vegetables to the letter. Use pesticides only and use of pesticides are regulat- ed by the United States Environmental Protection Agency

  16. EFFECTS OF DIFFERENT MILK TYPES AND STARTER CULTURES ON KEFIR

    Microsoft Academic Search

    Zübeyde Öner; Aynur Gül Karahan; Mehmet Lütfü Çakmakçi

    2009-01-01

    In this study, physicochemical and microbiological properties of kefir produced from cow, ewe and goat milk with grain and DVI cultures were investigated throughout 15 days storage period. Samples were taken after 1, 7 and 15 d of storage and acidity, pH, total solid as well as lactococci, lactobacilli and yeast counts were determined. Analyses of ethanol were carried out

  17. Festivals of the Darkest Days.

    ERIC Educational Resources Information Center

    Cacha, Frances B.

    1980-01-01

    Presents historical background on various winter festivals around the world including Saturnalia, Christmas, winter solstice, Yule festivals, Hannukah, Divali, and New Year's Day. Suggests how teachers can help elementary school students understand their own culture by studying these and other festivals using maps, mobiles, discussion, and reading…

  18. Atmospheric profiles during Dust days

    NASA Astrophysics Data System (ADS)

    Alpert, Pinhas; Stupp, Amnon; Osetinsky, Isabella; Ganor, Eli

    2013-04-01

    The vertical profiles of temperature, wind components, and humidity, for days with Saharan dust intrusions to Israel and with no dust were compared and analyzed. Three datasets, all for the 49-yr period of 1958-2006, were used: the daily dust observations at Tel Aviv, Israel, including about 1000 dust-days; the Eastern Mediterranean daily surface synoptic classification; the vertical data over the Eastern Mediterranean grid-point closest to Tel-Aviv at 32.5N 35E based on the NCEP/NCAR reanalysis. The meteorological parameters were averaged over the 49 year period by season, pressure level, synoptic-type, and dust and no-dust days. Prominent differences between dust and no-dust days were found for relative humidity and wind components during fall, winter and spring at 700, 600, and 500 hPa levels. Relative humidity was found to be higher during dust episodes. In Israel high RH at these levels is associated with precipitation. Absolute vertical velocity (Omega) values were higher on dust days than on days with no dust. Southerly and westerly components of wind were found to have higher values during dust days. It was found that for most synoptic systems, temperature below the 700-hPa level was equal or higher during dust days. Thus, during dust days the lower troposphere is unstable.

  19. A respirometer for organ cultures

    PubMed Central

    Lucas, D. R.

    1965-01-01

    1. A differential respirometer that can measure the oxygen uptake of organ cultures for periods of several days is described. 2. Diethanolamine is used as an external carbon dioxide buffer so that oxygen consumption can be measured in the presence of physiological concentrations of carbon dioxide and bicarbonate. 3. The efficiency of carbon dioxide retention with 5% carbon dioxide as gas phase is estimated to be 75% and the accuracy to be ±5% with a measured rate of oxygen uptake in excess of 40?l./hr. 4. Some experiments with guinea-pig retina are reported. ImagesFig. 2. PMID:5881664

  20. Sensory and chemical properties of white pickled cheese produced using kefir, yoghurt or a commercial cheese culture as a starter

    Microsoft Academic Search

    A. Goncu; Z. Alpkent

    2005-01-01

    We examined the sensory and chemical properties during a 120-day ripening period of white pickled cheeses produced using kefir, yoghurt, or commercial cheese cultures as the starter. Starter culture type had a significant impact on acidity, pH, salt, and fat levels in cheese (p0.01), as well as on ash level, appearance, and odour properties (p0.05). During ripening, culture type significantly

  1. A damped oscillator model of the 50-day oscillation in the length of the day

    Microsoft Academic Search

    Zhi-An Li; Clark R. Wilson

    1987-01-01

    The changes in the length of day with periods near 50 days appear to be driven by resonance in the atmospheric angular momentum. A damped harmonic oscillator model characterized by a resonance period and a Q-factor is used to describe this behavior. Estimates of the period and Q have been obtained by fitting autoregressive and autoregressive moving-average time series models

  2. Embryonic mouse pre-metatarsal development in organ culture

    NASA Technical Reports Server (NTRS)

    Klement, B. J.; Spooner, B. S.

    1993-01-01

    Embryonic mouse pre-metatarsals were removed from embryos at 13 days of gestation and cultured in a defined, serum-free medium for up to 15 days. By histological analysis, we observe that the cultured pre-metatarsal tissue undergoes a similar developmental profile as pre-metatarsals growing normally in vivo. The initial mesenchyme condensation regions undergo differentiation and morphogenesis to form distinct rods made up of cartilage tissue. A marker of this differentiation step is the synthesis of type II collagen. Metabolic labelling, pepsin digestion, SDS-PAGE, and autoradiography were used to demonstrate this protein when cartilage tissue is present in the cultures. After additional culture time, terminal chondrocyte differentiation and morphogenesis take place in specific regions of the cartilage rods to form bands of hypertrophied chondrocytes. One marker of this differentiation step is the synthesis of the enzyme alkaline phosphatase. We have measured the activity of this enzyme throughout the culture period and see a substantial increase at the time of terminal chondrocyte differentiation. Another feature of hypertrophied chondrocytes is that the matrix around the cells becomes calcified. Calcified matrix in our cultured pre-metatarsals was visualized by staining with alizarin red. By supplementing the defined culture medium with ITS, we observed that terminal chondrocyte differentiation took place in a shorter culture time. Supplementation of the medium with serum results in a similar acceleration of terminal differentiation, and, with additional culture time, an osteoid-like matrix forms around the central region of the rods.

  3. Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement.

    PubMed

    Nolan, P C; Nicholas, R M; Mulholland, B J; Mollan, R A; Wilson, D J

    1992-03-01

    We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on to demineralised human bone fragments and examined at ten-day intervals over a 50-day period by scanning electron microscopy. During this time the bank bone became progressively repopulated by the cultured osteoblasts. This system may offer a means of graft enhancement in elective orthopaedic and maxillofacial surgery by delivery of cultured autologous human osteoblasts to bone defects. PMID:1312095

  4. STS-79 Flight Day 5

    NASA Technical Reports Server (NTRS)

    1996-01-01

    On this fifth day of the STS-79 mission, the flight crew, Cmdr. William F. Readdy, Pilot Terrence W. Wilcutt, Mission Specialists, Thomas D. Akers, Shannon Lucid, Jay Apt, and Carl E. Walz, in the first full day of joint Shuttle/Mir operations begin in with the transfer of a biotechnology investigation and logistical supplies from Atlantis to Mir. The Biotechnology System, an investigation that will study the long-term development of cartilage cells in microgravity, was transported to Mir early this morning. During his planned four-month stay on Mir, John Blaha will take weekly samples of the culture which may provide researchers with information on engineering cartilage cells for possible use in transplantation. They also took time out of their schedules to talk with Good Morning America's Elizabeth Vargas in a brief interview. Prior to beginning the day's transfer activities, all nine astronauts and cosmonauts participated in a joint planning session to outline the day's schedule.

  5. Is Screening for Depression in the Perinatal Period Enough? The Co-Occurrence of Depression, Substance Abuse, and Intimate Partner Violence in Culturally Diverse Pregnant Women

    PubMed Central

    Hazen, Andrea L.; Baker-Ericzén, Mary J.; Landsverk, John; Horwitz, Sarah McCue

    2013-01-01

    Abstract Background The perinatal period provides unique opportunities to identify and intervene with the co-occurrence of perinatal depression, intimate partner violence (IPV), and substance use problems. Psychosocial screening recommended for women seen in maternal child health settings tends to target single rather than multiple risk factors; there is limited research examining the co-occurrence of these issues especially in racially and ethnically diverse women across the perinatal period. These analyses explore the relationships of sociodemographic, psychosocial, and behavioral characteristics in a large, diverse sample of women. Method Women receiving perinatal services at routinely scheduled visits, including the 6-week postpartum visit, were recruited from 10 community obstetric/gynecologic clinics. Data were collected on perinatal depression, IPV, maternal substance use, and sociodemographic characteristics by bilingual, bicultural research assistants. Results A total of 1868 women were screened, 1526 (82%) Latina, 1099 (58.8%) interviewed in Spanish; 20.4% (n=382) screened positive for depressive symptoms based on an Edinburgh Postnatal Depression Scale score of 10 or above, 20.9% reported harmful drinking, 4.3% reported drug use, 23% reported substance use problems, and 3.5% reported current or recent IPV. Women who were Black, Asian, Pacific Islander, or other race/ethnicity had greater odds for depressive symptoms relative to women who were Hispanic or Latino (odds ratio [OR]=1.81, p=0.005). Women reporting substance use problems (OR=2.37, p<0.0001) and IPV (OR=3.98, p<0.0001) had higher odds for depressive symptoms. Conclusion In a predominately Latina sample, 1 in 5 mothers (20.4%) screened positive for depressive symptoms and over one third (36.7%) reported one or more psychosocial issues during the perinatal period. Screening for multiple risk factors rather than just one can help clinicians tailor interventions for the successful management of psychosocial issues. PMID:23931153

  6. A cytological study of differentiation of the amnion and the effects of dimethyl sulfoxide (DMSO) on its structural moiety in Long Evans rat fetuses during periods day 101\\/2--191\\/2

    Microsoft Academic Search

    Jessie Kate Black

    1975-01-01

    Light, phase, and electron microscopic studies on the cytology of normal and dimethyl sulfoxide (DMSO)-treated amnii from days 101\\/2 through 191\\/2 of gestation were observed. This study emphasized the ontogeny of the structural moiety of normal Dimethyl sulfoxide (DMSO) induced various effects in amniotic tissue: cellular distortion, swelling of epithelial cells, endoplasmic reticuli and fibroblasts. Swelling and disruption of the

  7. Adult Day Care

    MedlinePLUS

    ... Resize Text Printer Friendly Online Chat Adult Day Care What is Adult Day Care? How Do Adult ... More About Local Services? What is Adult Day Care? Adult Day Care Centers are designed to provide ...

  8. Effects of Days Dry and Days Open on Holstein Milk Production

    Microsoft Academic Search

    L. R. Schaeffer; C. R. Henderson

    1972-01-01

    Holstein lactation records from the Dairy Herd Improvement test day files of the New York Dairy Records Processing Lab- oratory at Cornell University were used to study genetic and environmental relation- ships of days dry and days open with milk production. Age and month of calving significantly influenced length of dry period. Within herd heritability estimates of days dry were

  9. Bioactivity of water soluble extracts and some characteristics of white cheese during the ripening period as effected by packaging type and probiotic adjunct cultures.

    PubMed

    Erkaya, Tuba; ?engul, Mustafa

    2015-02-01

    In this study, the chemical composition, proteolysis and in vitro angiotensin-converting enzyme-(ACE)-inhibitory and antioxidant activities of white cheeses made using probiotic adjunct cultures (Bifidobacterium bifidum DSMZ 20456 and Lactobacillus acidophilus DSMZ 20079) were investigated. The cheeses were ripened in a vacuum package or brine for 120 d at 4 °C. The cheese samples maintained the probiotic characteristics of the viable cells as >106 cfu/g even after ripening for 120 d. The proteolysis degrees in terms of water-soluble nitrogen/total nitrogen (WSN/TN), trichloroacetic acid-soluble nitrogen/total nitrogen (TCA-SN/TN) and phosphotungstic acid-soluble nitrogen/total nitrogen (PTA-SN/TN) values in the cheeses increased throughout the ripening. The highest levels of proteolysis were found in cheese made using Lb. acidophilus DSMZ 20079 and ripened in a vacuum package. ACE-inhibitory activity of the water soluble extracts (WSEs) of the cheeses increased significantly (P < 0·05) throughout the ripening (IC50 values 82·78-140·99 ?g/ml). Use of Lb. acidophilus DSMZ 20079 and packaging under vacuum significantly increased the percentage of ACE inhibiting activity. WSEs had DPPH scavenging activity (the IC50 values were 2·41-5·39 mg/ml and the inhibition values were 5·10-10·38%), increasing up to 60 d ripening. In the present study, it was observed that Lb. acidophilus DSMZ 20079 was more effective than Bifido. bifidum DSMZ 20456 in terms of the cheese characteristics investigated. PMID:25592630

  10. Periodic functions with variable period

    E-print Network

    M. V Pryjmak

    2010-06-08

    The examples of rhythmical signals with variable period are considered. The definition of periodic function with the variable period is given as a model of such signals. The examples of such functions are given and their variable periods are written in the explicit form. The system of trigonometric functions with the variable period is considered and its orthogonality is proved. The generalized system of trigonometric functions with the variable period is also suggested; some conditions of its existence are considered.

  11. Lactic Acid Production in a Mixed-Culture Biofilm Reactor †

    PubMed Central

    Demirci, Ali; Pometto, Anthony L.; Johnson, Kenneth E.

    1993-01-01

    Novel solid supports, consisting of polypropylene blended with various agricultural materials (pp composite), were evaluated as supports for pure- and mixed-culture continuous lactic acid fermentations in biofilm reactors. Streptomyces viridosporus T7A (ATCC 39115) was used to form a biofilm, and Lactobacillus casei subsp. rhamnosus (ATCC 11443) was used for lactic acid production. For mixed-culture fermentations, a 15-day continuous fermentation of S. viridosporus was performed initially to establish the biofilm. The culture medium was then inoculated with L. casei subsp. rhamnosus. For pure-culture fermentation, L. casei subsp. rhamnosus was inoculated directly into the reactors containing sterile pp composite chips. The biofilm reactors containing various pp composite chips were compared with a biofilm reactor containing pure polypropylene chips and with a reactor containing a suspension culture. Continuous fermentation was started, and each flow rate (0.06 to 1.92 ml/min) was held constant for 24 h; steady state was achieved after 10 h. Lactic acid production was determined throughout the 24-h period by high-performance liquid chromatography. Production rates that were two to five times faster than those of the suspension culture (control) were observed for the pure- and mixed-culture bioreactors. Both lactic acid production rates and lactic acid concentrations in the culture medium were consistently higher in mixed-culture than in pure-culture fermentations. Biofilm formation on the chips was detected at harvest by chip clumping and Gram staining. PMID:16348843

  12. The Impact of Day Care

    PubMed Central

    Alexander, Jean

    1983-01-01

    Children who attend day care centres have different behavioral characteristics than children cared for at home by parents. Several studies report that children who have attended day care are more aggressive, more physically active, less cooperative, interact more with their peers, and are slower in acquiring adults' cultural values than children cared for at home. While children from low risk families appear to gain no cognitive advantage from day care, those from high risk families or with developmental problems do. Problems with hearing, vision, development or behavior, and child abuse may be identified in a well organized centre. Early recognition of developmental problems may help ensure the child does not lack self-worth later on. Imagesp1880-ap1881-a PMID:21283426

  13. DARPA Robotics Proposers' Day

    E-print Network

    DARPA Robotics Challenge Proposers' Day April 16, 2012 #12;2 DARPA Robotics Challenge Proposers' Day The Proposers' Day will begin at 12:10 PM EDT April 16, 2012 #12;3 DARPA Robotics Challenge Proposers' Day Dr. Gill Pratt Program Manager, DARPA #12;DARPA Robotics Challenge Proposers' Day Agenda 4 12

  14. An integrated system of solar light, artificial light and organic carbon supply for cyclic photoautotrophic-heterotrophic cultivation of photosynthetic cells under day–night cycles

    Microsoft Academic Search

    James C. Ogbonna; Toshihiko Soejima; Charles U. Ugwu; Hideo Tanaka

    2001-01-01

    An integrated system of solar light, artificial light and organic carbon supply was developed for cyclic photoautotrophic-heterotrophic cultivation of photosynthetic cells. The energy source for the culture is automatically switched to solar light energy (when the weather is sunny), to artificial light energy (during the cloudy period of the day) or to organic carbon source (at night). Thus minimum amount

  15. Variability in storage potential of banana shoot cultures under medium term storage conditions

    Microsoft Academic Search

    Ines Van den houwe; Kris De Smet; Hugues Tezenas Montcel; Rony Swennen

    1995-01-01

    Shoot cultures of 401 banana clones were conserved under slow growth conditions (16±1°C, 25µmol m-2 s-1). Storage duration-defined as 60% survival time of 20 shoot cultures of a clone-averaged 334 days. However, large differences occurred among the different genomic (sub)groups and even within the same (sub)group. East-African highland bananas and non-plantain AAB bananas can be stored for significantly longer periods.

  16. Comparison of white-rot fungi cultures for decolorization of textile dyestuffs

    Microsoft Academic Search

    I. Kapdan; F. Kargi; G. McMullan; R. Marchant

    2000-01-01

    Four different white rot fungi cultures were used for decolorization of five different textile dyestuffs. Phanerochaeta chrysosporium 671.71, P. chrysosporium MUCL, Coriolus versicolor and C. versicolor MUCL cultures effectively decolorized Everzol Yellow 4GL, Everzol Red RBN, D. Orange K-GL, Everdirect Supra yellow PG and Everzol Turquoise Blue G textile dyestuffs in shake flasks within 9 days of incubation period. Decolorization

  17. Effect of Fibroblast Co-culture on In Vitro Maturation and Fertilization of Mouse Preantral Follicles

    PubMed Central

    Heidari, Mahmoud; Malekshah, Abbasali Karimpour; Parivar, Kazem; Khanbabaei, Ramezan; Rafiei, Alireza

    2011-01-01

    Background The aim of this study was to evaluate fibroblast co-culture on in vitro maturation and fertilization of prepubertal mouse preantral follicles. Materials and Methods The ovaries of 12-14 day old mice were dissected and 120-150 ?m intact preantral follicles with one or two layers of granulosa cells, and round oocytes were cultured individually in ?-minimal essential medium (?-MEM) supplemented with 5% fetal bovine serum (FBS), 100 mIU/ml recombinant follicle stimulating hormone, 1% insulin, transferrin, selenium mix, 100 ?g/ml penicillin and 50 ?g/ml streptomycin as base medium for 12 days. A total number of 226 follicules were cultured under two conditions: i) base medium as control group (n=113); ii) base medium co-cultured with mouse embryonic fibroblast (MEF) (n=113). Follicular diameters, alone, in addition to other factors were analyzed by student’s t-test and chi-square test, respectively. Results The co-culture group showed significant differences (p<0.05) in growth rate (days 4, 6 and 8 of the culture period) and survival rate. However, there was no significant difference in antrum formation, ovulation rate and embryonic development of released oocytes. There were significant differences (p<0.05) in the estradiol and progesterone secretion at all days between the co-culture and control groups. Conclusion Fibroblast co-culture increased survival rate and steroid production of preantral follicles by promoting granulosa cell proliferation. PMID:24917917

  18. First Day of Class

    NSDL National Science Digital Library

    Compiled by Carol Ormand, SERC, Carleton College Check out a set of videos of what faculty in several disciplines do on their first day of class. Why is the first day important? It's the first day of class, ...

  19. Stress-induced changes in the expression of the clock protein PERIOD1 in the rat limbic forebrain and hypothalamus: role of stress type, time of day, and predictability.

    PubMed

    Al-Safadi, Sherin; Al-Safadi, Aya; Branchaud, Marie; Rutherford, Spencer; Dayanandan, Arun; Robinson, Barry; Amir, Shimon

    2014-01-01

    Stressful events can disrupt circadian rhythms in mammals but mechanisms underlying this disruption remain largely unknown. One hypothesis is that stress alters circadian protein expression in the forebrain, leading to functional dysregulation of the brain circadian network and consequent disruption of circadian physiological and behavioral rhythms. Here we characterized the effects of several different stressors on the expression of the core clock protein, PER1 and the activity marker, FOS in select forebrain and hypothalamic nuclei in rats. We found that acute exposure to processive stressors, restraint and forced swim, elevated PER1 and FOS expression in the paraventricular and dorsomedial hypothalamic nuclei and piriform cortex but suppressed PER1 and FOS levels exclusively in the central nucleus of the amygdala (CEAl) and oval nucleus of the bed nucleus of the stria terminalis (BNSTov). Conversely, systemic stressors, interleukin-1? and 2-Deoxy-D-glucose, increased PER1 and FOS levels in all regions studied, including the CEAl and BNSTov. PER1 levels in the suprachiasmatic nucleus (SCN), the master pacemaker, were unaffected by any of the stress manipulations. The effect of stress on PER1 and FOS was modulated by time of day and, in the case of daily restraint, by predictability. These results demonstrate that the expression of PER1 in the forebrain is modulated by stress, consistent with the hypothesis that PER1 serves as a link between stress and the brain circadian network. Furthermore, the results show that the mechanisms that control PER1 and FOS expression in CEAl and BNSTov are uniquely sensitive to differences in the type of stressor. Finally, the finding that the effect of stress on PER1 parallels its effect on FOS supports the idea that Per1 functions as an immediate-early gene. Our observations point to a novel role for PER1 as a key player in the interface between stress and circadian rhythms. PMID:25338089

  20. Period Cramps

    MedlinePLUS

    ... Games Kids' Medical Dictionary En Español What Other Kids Are Reading Girls and Puberty Boys and Puberty ... help push the blood out through a girl's vagina during her period . You probably know periods usually ...

  1. Cultural Calendar

    NSDL National Science Digital Library

    2010-01-01

    This activity combines cultural exploration with number patterns and systems. Choose a culture or historical period and have learners make a calendar for a month in that time. For more challenge, pick a country that uses different numerals, such as India or China or go back in time and make calendars for a month in ancient Mayan times, using the Mayan number system. Ask learners to compare: How are the calendars they’ve made different from the calendars we use? How are they the same? What patterns do you notice? Available as a web page and downloadable PDF. Blank calendar template is also available as a downloadable PDF. Students should be able to write numerals to 30 and have some familiarity with calendars.

  2. Consultation in Day Care.

    ERIC Educational Resources Information Center

    Kiester, Dorothy J.

    This handbook clarifies the responsibility, role and functions of the day care consultant. A chapter on the philosophy of day care is intended to stimulate thoughtful consideration of how existing patterns of day care affect children, parents, and the family. A variety of methods and strategies for translating day care philosophy into practice are…

  3. CHILOMASTIX MESNILI AND A METHOD FOR ITS CULTURE

    PubMed Central

    Boeck, William C.

    1921-01-01

    1. Cases of flagellate infection of man by Chilomastix mesnili have been reported from nearly every locality in the world. They are fairly abundant in the United States and are often confused with cases of infection by Trichomonas intestinalis. 2. The shape of Chilomastix is pyriform. The body contains the following structures: nucleus, centrosome, primary, secondary, and tertiary blepharoplasts, parabasal, parastyle, peristomal fiber, ventral cytostome, cytostomal flagellum, three anterior flagella, food vacuoles with bacterial inclusions, and a posterior spine-like process, the hold-fast organ. 3. The twisted shape in some flagellates in this case of infection is a temporary condition. It was seen in flagellates taken from the feces of the patient on only two occasions and was never observed among the cultured forms. 4. The cysts of Chilomastix are lemon-shaped and occurred irregularly in the stools of the patient. They have not been encountered thus far in the cultures. 5. Chilomastix mesnili was cultured continuously from January 27 to June 15, 1920, in an artificial medium composed of one part of human serum and four parts of Locke's solution with the addition of a small amount of dextrose. 6. Cultures sometimes remained viable for a period of 1 to 10 days, but generally for a period of 1 to 8 days. 7. The flagellates in the cultures increased in number during the 1st, 2nd, 3rd, and often the 4th days, after which there was a decrease until all had disappeared. 8. A period of lag, probably 13 hours in length, and followed by a period of great fecundity, characterized the growth of the flagellates during the 1st day in the cultures. 9. The number of flagellates in the cultures varied considerably and often reached 700,000 per cubic centimeter. 10. The bacteria served in part as the food of the flagellates. The bacterial organisms identified in the cultures were Bacillus coli communis, Bacillus coli communior, Bacterium aerogenes, Bacillus alkaligenes, and Bacillus proteus. The proteins of the blood serum, the mineral salts, and the dextrose in the medium may also have proved a source of food for the protozoa. 11. The products of bacterial metabolism, resulting from the growth of the bacteria, their fermentative activity, and their death may explain the rate of growth, reproduction, and death of the flagellates in the cultures. A certain amount of the products of decomposition together with the presence of the bacterial food supply may explain the rapid proliferation of the flagellates during the 1st day and the slower rate up to the 3rd or 4th day. An excess of these products may have inhibited the growth and multiplication of the protozoa, especially after the 3rd or 4th day, and ultimately may have brought about their death and disappearance from the cultures. 12. The initial alkalinity of the medium increased at the death of the culture. It is not believed that the change in alkalinity proved lethal to the protozoa. 13. Binary fission was often observed. The plane of division was median and longitudinal. A paradesmose was formed. 14. Multiple fission also occurred. The flagellate body, ameboid in movement, was a somatella comprised of four zooids. It contained four cytostomes, each with a cytostomal flagellum. The number of anterior flagella was not complete for each zooid. 15. Flagellates, joined in pairs, were often seen during the first 3 days in the cultures. They may represent conjugating forms, but as yet no evidence of the exchange of nuclear substance has been seen. PMID:19868485

  4. Czech Culture in Prague: Architecture

    Microsoft Academic Search

    Kyuchin Kim

    2003-01-01

    Prague's main feature is that, out of many cultural treasures, it preserved its architectural culture and put it to practical use to present day. Particularly Prague has embraced a wealth of architectural styles from many ages. From the Romanesque, the Gothic culture of Czech's pinnacle age, Baroque, Neo Classicism, the Art Nouveau style buildings that concentrated in Prague at the

  5. Prospective study of blood culture during transesophageal echocardiography.

    PubMed

    Shyu, K G; Hwang, J J; Lin, S C; Tzou, S S; Cheng, J J; Kuan, P; Lien, W P

    1992-12-01

    To ascertain the incidence and significance of bacteremia associated with transesophageal echocardiography (TEE), 132 consecutive patients (aged 17 to 73 years) free of apparent infection who were undergoing 135 transesophageal echocardiographic procedures from October 1990 to August 1991, were prospectively studied. For each procedure, two sets of blood cultures were obtained for culture 30 to 60 minutes before TEE, immediately after, and 180 to 240 minutes after the procedure. For each blood culture, 10 ml of venous blood was evenly inoculated into aerobic and anaerobic culture bottles and inoculated for 7 days using a radiometric system. A throat swab was obtained immediately before each procedure. Three of 270 preprocedure blood cultures were positive for Bacillus cereus, Staphylococcus simulans, and Peptostreptococcus species, respectively. No blood culture was positive in the immediate postprocedure period. Two of 270 late blood samples grew Staphylococcus epidermidis in the same patient. Nevertheless, the microorganisms isolated from blood cultures were different from those isolated from the throat swab. No patients had fever or evidence of infective endocarditis after TEE during the follow-up period. It is concluded that the incidence of TEE-related bacteremia is extremely low, and a general recommendation for antibiotic prophylaxis during TEE is not warranted. PMID:1462911

  6. Periodic Table

    NSDL National Science Digital Library

    Using the Macromedia Shockwave Player, this American Chemical Society website offers three interactive periodic tables. Students can find the basic data on each element including its atomic radius, stable isotopes, melting point, and density in the first periodic table tab. The website identifies different elemental groups by color. Users can view the electron configuration by selecting elements on the periodic table in the second tab. The last tab offers plots of the elements' electronegativity, ionization energy, and other properties.

  7. Enhanced Osteogenesis of Human Mesenchymal Stem Cells by Periodic Heat Shock in Self-Assembling Peptide Hydrogel

    PubMed Central

    Chen, Jing; Shi, Zhong-Dong; Ji, Xinying; Morales, Jorge; Zhang, Jingwei; Kaur, Navneet

    2013-01-01

    The mechanisms for the heat-induced osteogenesis are not completely known and the thermal regulation of human mesenchymal stem cell (hMSC) differentiation is not well studied. In this study, the direct effects of mild heat shock (HS) on the differentiation of hMSCs into osteoblasts in self-assembling peptide hydrogel and on tissue culture plates were investigated. hMSCs isolated from human bone marrow were seeded in conventional culture plates (two-dimensional [2D] culture) and on the surface of three-dimensional (3D) PuraMatrix peptide hydrogel (3D culture), followed by 1?h HS at 41°C once a week during osteogenic differentiation. Alkaline phosphatase (ALP) activity was enhanced in both 2D and 3D cultures via periodic HS at early stage of differentiation; meanwhile, HS significantly increased the calcium deposition at day 19 and 27 of differentiation in both 2D and 3D cultures. The periodic HS also upregulated osteo-specific genes, osterix (OSX) on day 11, osteopontin (OP) on day 19, and bone morphogenetic protein 2 (BMP2) on day 25 in 2D culture. In 3D PuraMatrix culture, the runt-related transcription factor 2 (Runx2) was upregulated by HS on day 25 of differentiation. The heat shock protein 70 (HSP70) was significantly upregulated by HS in differentiated hMSCs analyzed at 24?h after HS. These results demonstrate that HS induced an earlier differentiation of hMSCs and enhanced the maturation of osteoblasts differentiated from hMSCs. Therefore, mild HS treatment may be potentially used to enhance the bone regeneration using hMSCs. Our data will guide the design of in vivo heating protocols and enable further investigations in thermal treatments of MSC osteogenesis for bone tissue engineering. PMID:23072422

  8. Enhanced osteogenesis of human mesenchymal stem cells by periodic heat shock in self-assembling peptide hydrogel.

    PubMed

    Chen, Jing; Shi, Zhong-Dong; Ji, Xinying; Morales, Jorge; Zhang, Jingwei; Kaur, Navneet; Wang, Sihong

    2013-03-01

    The mechanisms for the heat-induced osteogenesis are not completely known and the thermal regulation of human mesenchymal stem cell (hMSC) differentiation is not well studied. In this study, the direct effects of mild heat shock (HS) on the differentiation of hMSCs into osteoblasts in self-assembling peptide hydrogel and on tissue culture plates were investigated. hMSCs isolated from human bone marrow were seeded in conventional culture plates (two-dimensional [2D] culture) and on the surface of three-dimensional (3D) PuraMatrix peptide hydrogel (3D culture), followed by 1?h HS at 41°C once a week during osteogenic differentiation. Alkaline phosphatase (ALP) activity was enhanced in both 2D and 3D cultures via periodic HS at early stage of differentiation; meanwhile, HS significantly increased the calcium deposition at day 19 and 27 of differentiation in both 2D and 3D cultures. The periodic HS also upregulated osteo-specific genes, osterix (OSX) on day 11, osteopontin (OP) on day 19, and bone morphogenetic protein 2 (BMP2) on day 25 in 2D culture. In 3D PuraMatrix culture, the runt-related transcription factor 2 (Runx2) was upregulated by HS on day 25 of differentiation. The heat shock protein 70 (HSP70) was significantly upregulated by HS in differentiated hMSCs analyzed at 24?h after HS. These results demonstrate that HS induced an earlier differentiation of hMSCs and enhanced the maturation of osteoblasts differentiated from hMSCs. Therefore, mild HS treatment may be potentially used to enhance the bone regeneration using hMSCs. Our data will guide the design of in vivo heating protocols and enable further investigations in thermal treatments of MSC osteogenesis for bone tissue engineering. PMID:23072422

  9. Day and Night Sky

    NSDL National Science Digital Library

    Lindsay Demoranville

    2012-07-10

    What objects can you see in the day sky? What objects can you see in the night sky? Are there objects that can be seen in both the day and night sky? Can the sun be seen during the night, or only during the day? Students will encounter these questions as they explore the similarities and differences between the day and night sky. This lesson will help students identify what objects can be seen in the day and night sky, and objects are exclusively seen in the day sky and the night sky.

  10. Carbon disulphide production in laboratory cultures of marine phytoplankton

    NASA Astrophysics Data System (ADS)

    Xie, Huixiang; Scarratt, Michael G.; Moore, Robert M.

    Carbon disulphide (CS 2) data were collected from axenic monocultures of six species of marine phytoplankton. The tested species included Chaetoceros calcitrans, Phaeodactylum tricornutum, Phaeocystis sp., Porphyridium purpureum, Synechococcus sp. and Isochrysis sp. For a period of between two weeks and forty days, substantial accumulation of CS 2 was found in the cultures of C. calcitrans, P. tricornutum and Phaeocystis sp., whereas the change of CS 2 concentration in the remaining cultures was insignificant. C. calcitrans had a potential for CS 2 production about 10 times higher than P. tricornutum or Phaeocystis sp. The formation of the compound was strongly dependent on the physiological state of the cultured species. More investigation is needed to elucidate the mechanisms responsible for the formation of this sulphur compound in these cultures.

  11. Cultural Characteristics of Shimizuomyces paradoxus Collected from Korea

    PubMed Central

    Sung, Gi-Ho; Shrestha, Bhushan; Park, Ki-Byung

    2010-01-01

    This study investigated the cultural characteristics of Shimizuomyces paradoxus in different nutritional and environmental conditions. The highest mycelial growth was observed in Schizophyllum (mushroom) genetics complete medium plus yeast extract agar medium, and the optimal temperature and pH were 25? and pH 8.0, respectively. The optimal carbon and nitrogen sources were 1% dextrose and 1% peptone in agar. However, in liquid culture the highest dry mycelium weight was found for the potato dextrose agar and potato sucrose agar broths. The optimum inoculum size was five mycelial discs (5 mm) per 100 mL of broth, and the optimum liquid culture period was 25 days. This is the first ever report of S. paradoxus cultural characteristics. PMID:23956653

  12. Generation of T-cell function in organ culture of foetal mouse thymus. II. Mixed lymphocyte culture reactivity.

    PubMed Central

    Robinson, J H; Owen, J J

    1977-01-01

    The generation of MLC reactive cells in organ cultures of embryonic mouse thymus is described. At least 4 days in culture are required before MLC reactive thymocytes are detectable in this system, which is in marked contrast to the short incubation period required for the expression of Thy-1 on T-cell precursors in other systems. Our data demonstrates the generation of T-cell subsets independent of influences from peripheral lymphoid organs, and in the absence of B cells or their products. One very important application of this technique is in the investigation of the mechanism of T-cell tolerance to self. PMID:139222

  13. Irregular Periods

    MedlinePLUS

    ... Date reviewed: October 2013 Back 1 ? 2 For Teens For Kids For Parents MORE ON THIS TOPIC Gyn Checkups I'm 14 and I Don't Have My Period Yet. Is This Normal? Can a Girl Get Pregnant if She Has Sex During Her Period? Birth Control Pill Coping With ...

  14. Culture shock and travelers.

    PubMed

    Stewart, L; Leggat, P A

    1998-06-01

    As travel has become easier and more affordable, the number of people traveling has risen sharply. People travel for many and varied reasons, from the business person on an overseas assignment to backpackers seeking new and exotic destinations. Others may take up residence in different regions, states or countries for family, business or political reasons. Other people are fleeing religious or political persecution. Wherever they go and for whatever reason they go, people take their culture with them. Culture, like language, is acquired innately in early childhood and is then reinforced through formal and complex informal social education into adulthood. Culture provides a framework for interpersonal and social interactions. Therefore, the contact with a new culture is often not the exciting or pleasurable experience anticipated. When immersed in a different culture, people no longer know how to act when faced with disparate value systems. Contact with the unfamiliar culture can lead to anxiety, stress, mental illness and, in extreme cases, physical illness and suicide. "Culture shock" is a term coined by the anthropologist Oberg. It is the shock of the new. It implies that the experience of the new culture is an unpleasant surprise or shock, partly because it is unexpected and partly because it can lead to a negative evaluation of one's own culture. It is also known as cross-cultural adjustment, being that period of anxiety and confusion experienced when entering a new culture. It affects people intellectually, emotionally, behaviorally and physically and is characterized by symptoms of psychological distress. Culture shock affects both adults and children. In travelers or workers who have prolonged sojourns in foreign countries, culture shock may occur not only as they enter the new culture, but also may occur on their return to their original culture. Children may also experience readjustment problems after returning from leading sheltered lives in expatriate compounds. This readjustment back to their own culture after a period of time abroad has been termed "reverse culture shock, a condition which has been studied in both corporate managers and Peace Corps volunteers. With culture shock and many other processes of psychological adjustment, people tend to suffer alone, thinking that they are the only ones not coping well with their new circumstance. The objective of this paper was to bring the phenomenon of culture shock to the awareness of travel health advisors, who can in turn advise travelers, especially longer term travelers, about having realistic expectations of their travel and life in new cultures. PMID:9772322

  15. Riley-Day syndrome

    MedlinePLUS

    Riley-Day syndrome is an inherited disorder that affects nerves throughout the body. ... Riley-Day syndrome is passed down through families (inherited). A person must inherit a copy of the defective gene ...

  16. Mother's Day Card

    NSDL National Science Digital Library

    2003-01-01

    This activity page which supports the Cyberchase video, "Saving Mother's Day" uses lines of symmetry to create a Madre Bonita flower, the symbol of Mother's Day. This activity can be printed for student use.

  17. Rethinking Opening Day

    ERIC Educational Resources Information Center

    King, Matthew

    2005-01-01

    In this article, the author discusses what a superintendent can do to energize the faculty on opening day. He uses a creative approach in developing the opening day speech--and keeps the teachers guessing. Believing that the superintendent should not be the focal point of opening day, he instead gives teachers the opportunity to share their…

  18. The Individual Day Trader

    Microsoft Academic Search

    Juhani Linnainmaa

    2005-01-01

    Abstract This paper shows that individual day traders are reluctant to close losing day trades. They even sell other stocks from their portfolios to finance the unintended purchases. This disposition to ride losers has significant long-term welfare consequences. Day traders hurt their portfolios’ performance up to ?6% in three months after a holdings change. The changes in individuals’ exposure to

  19. Every Day Is Mathematical

    ERIC Educational Resources Information Center

    Barger, Rita H.; Jarrah, Adeeb M.

    2012-01-01

    March 14 is special because it is Pi Day. Mathematics is celebrated on that day because the date, 3-14, replicates the first three digits of pi. Pi-related songs, websites, trivia facts, and more are at the fingertips of interested teachers and students. Less celebrated, but still fairly well known, is National Metric Day, which falls on October…

  20. Adult Day Services

    MedlinePLUS

    ... 3 $35-45 $60-70 $130-140 Adult Day Services $75,000 $50,000 $25,000 $0 Adult Day Services At-a-Glance • Families share in the ... as many locations 5 • More funding for adult day services decreases Medicaid costs by reducing full-time ...

  1. WORD. A. DAY

    NSDL National Science Digital Library

    A. WORD. A. DAY. is the Web page for the mailing list A.Word A Day (AWAD), which mails out a vocabulary word and its definition (with occasional commentary) to the subscribers every day. Currently AWAD has over 10,000 subscribers in 53 countries.

  2. Share This: Plastic Day

    E-print Network

    Cakoni, Fioralba

    of Delaware's Earth Week activities surrounding the observance of the 42nd Earth Day on April 22. DENINShare This: Upcoming Events April 18 Plastic Day: Book Discussion & Film April 28 Ag Day The DENIN to strengthen our efforts to understand, predict, and adjust to Earth's changing climate. One mild winter does

  3. Endogenous amyloidogenesis in long-term rat hippocampal cell cultures

    PubMed Central

    2011-01-01

    Background Long-term primary neuronal cultures are a useful tool for the investigation of biochemical processes associated with neuronal senescence. Improvements in available technology make it possible to observe maturation of neural cells isolated from different regions of the rodent brain over a prolonged period in vitro. Existing experimental evidence suggests that cellular aging occurs in mature, long-term, primary neuronal cell cultures. However, detailed studies of neuronal development in vitro are needed to demonstrate the validity of long-term cell culture-based models for investigation of the biochemical mechanisms of in vitro neuronal development and senescence. Results In the current study, neuron-enriched hippocampal cell cultures were used to analyze the differentiation and degeneration of hippocampal neurons over a two month time period. The expression of different neuronal and astroglial biomarkers was used to determine the cytochemical characteristics of hippocampal cells in long-term cultures of varying ages. It was observed that the expression of the intermediate filament nestin was absent from cultures older than 21 days in vitro (DIV), and the expression of neuronal or astrocytic markers appeared to replace nestin. Additionally, morphological evaluations of neuronal integrity and Hoescht staining were used to assess the cellular conditions in the process of hippocampal culture development and aging. It was found that there was an increase in endogenous production of A?1-42 and an increase in the accumulation of Congo Red-binding amyloidal aggregates associated with the aging of neurons in primary culture. In vitro changes in the morphology of co-existing astrocytes and cell culture age-dependent degeneration of neurodendritic network resemble features of in vivo brain aging at the cellular level. Conclusion In conclusion, this study suggests that long-term primary CNS culture is a viable model for the study of basic mechanisms and effective methods to decelerate the process of neuronal senescence. PMID:21569253

  4. Post-translational control of collagen fibrillogenesis in mineralizing cultures of chick osteoblasts

    NASA Technical Reports Server (NTRS)

    Gerstenfeld, L. C.; Riva, A.; Hodgens, K.; Eyre, D. R.; Landis, W. J.

    1993-01-01

    Cultured osteoblasts from chick embryo calvaria were used as a model system to investigate the post-translational extracellular mechanisms controlling the macroassembly of collagen fibrils. The results of these studies demonstrated that cultured osteoblasts secreted a collagenous extracellular matrix that assembled and mineralized in a defined temporal and spatial sequence. The assembly of collagen occurred in a polarized fashion, such that successive orthogonal arrays of fibrils formed between successive cell layers proceeding from the culture surface toward the media. Mineralization followed in the same manner, being observed first in the deepest and oldest fibril layers. Collagen fibrillogenesis, the kinetics of cross-link formation, and collagen stability in the extracellular matrix of the cultures were examined over a 30 day culture period. Between days 8 and 12 in culture, collagen fibril diameters increased from < 30 nm to an average of 30-45 nm. Thereafter, diameters ranged in size from 20 to 200 nm. Quantitation of the collagen cross-linking residues, hydroxylysyl pyridinoline (HP) and lysyl pyridinoline (LP), showed that these mature cross-links increased from undetectable levels to concentrations found in normal chick bone. Analysis of the kinetics of their formation by pulse-chase labeling the cultures with [3H]lysine showed a doubling time of approximately 5 days. The relationships between cross-link formation, fibrillogenesis, and collagen stability were examined in cultures treated with beta-aminopropionitrile (beta-APN), a potent inhibitor of lysyl oxidase and cross-link formation. In beta-APN-treated cultures, total collagen synthesis was increased twofold, with no change in mRNA levels for type I collagen, whereas the amount of collagen accumulated in the cell layer was decreased by 50% and mineral deposition was reduced. The rate of collagen retention in the matrix was assessed by pulse-chase analysis of [3H]proline over a 16 day period in control and beta-APN-treated cultures. In control cultures, about 20% of the labeled collagen was lost from the cell layers over a 16 day period compared with > 80% in the presence of beta-APN. The beta-APN-treated cultures also showed a wider diversity of fibril diameters with a median in the > 45-60 nm range. In summary, these data suggest that cross-linking and assembly of collagen fibrils secreted by osteoblasts in vitro occur in a fashion similar to that found in vivo. The rate of cross-link formation is relatively constant and may be correlated with increasing collagen mass.(ABSTRACT TRUNCATED AT 400 WORDS).

  5. The culture of care within psychiatric services: tackling inequalities and improving clinical and organisational capabilities

    PubMed Central

    2012-01-01

    Introduction Cultural Consultation is a clinical process that emerged from anthropological critiques of mental healthcare. It includes attention to therapeutic communication, research observations and research methods that capture cultural practices and narratives in mental healthcare. This essay describes the work of a Cultural Consultation Service (ToCCS) that improves service user outcomes by offering cultural consultation to mental health practitioners. The setting is a psychiatric service with complex and challenging work located in an ethnically diverse inner city urban area. Following a period of 18 months of cultural consultation, we gather the dominant narratives that emerged during our evaluation of our service. Results These narratives highlight how culture is conceptualized and acted upon in the day-to-day practices of individual health and social care professionals, specialist psychiatric teams and in care systems. The findings reveal common narratives and themes about culture, ethnicity, race and their perceived place and meaningfulness in clinical care. These narratives express underlying assumptions and covert rules for managing, and sometimes negating, dilemmas and difficulties when considering “culture” in the presentation and expression of mental distress. The narratives reveal an overall “culture of understanding cultural issues” and specific “cultures of care”. These emerged as necessary foci of intervention to improve service user outcomes. Conclusion Understanding the cultures of care showed that clinical and managerial over-structuring of care prioritises organisational proficiency, but it leads to inflexibility. Consequently, the care provided is less personalised and less accommodating of cultural issues, therefore, professionals are unable to see or consider cultural influences in recovery. PMID:23020856

  6. Uptake and distribution of rare earth elements in rice seeds cultured in fertilizer solution of rare earth elements

    Microsoft Academic Search

    Dongan Yuan; Xiao-quan Shan; Qing Huai; Bei Wen; Xinru Zhu

    2001-01-01

    The uptake behavior of rare earth elements (REEs) under pot conditions using deionized water and a REE fertilizer solution as the culture media as well as the distribution of REEs in rice proteins were studied. The uptake of REEs in rice seeds increased dramatically after a lag period of approximately three days. Roots can accumulate a much higher content of

  7. Open Day at SHMI.

    NASA Astrophysics Data System (ADS)

    Jarosova, M.

    2010-09-01

    During the World Meteorological Day there has been preparing "Open Day" at Slovak Hydrometeorological Institute. This event has more than 10 years traditions. "Open Day" is one of a lot of possibilities to give more information about meteorology, climatology, hydrology too to public. This "Day" is executed in whole Slovakia. People can visit the laboratories, the forecasting room....and meteo and clima measuring points. The most popular is visiting forecasting room. Visitors are interested in e.g. climatologic change in Slovakia territory, preparing weather forecasting, dangerous phenomena.... Every year we have more than 500 visitors.

  8. The Sun's Period of Rotation

    NSDL National Science Digital Library

    In this activity, students learn how sunspots can be used to determine the rotation period of the Sun. They are asked to theorize on possible reasons for the Sun's apparent motion, and to use an overlay to determine solar latitude and longitude and photographs of sunspots to help them perform measurements to determine the rotation period of the Sun. The overlay, photos of sunspots over a period of four days, and a student worksheet are provided.

  9. Invasive Pneumococcal Disease in Infants Younger Than 90 Days Before and After Introduction of PCV7

    PubMed Central

    Olarte, Liset; Ampofo, Krow; Stockmann, Chris; Mason, Edward O.; Daly, Judy A.; Pavia, Andrew T.

    2013-01-01

    BACKGROUND: Introduction of the heptavalent pneumococcal conjugate vaccine (PCV7) changed the epidemiology of invasive pneumococcal disease (IPD). We evaluated the changes that occurred after PCV7 introduction among Utah infants aged 1 to 90 days, too young to be fully immunized. METHODS: We identified children <18 years with culture-confirmed IPD from 1997–2010. We analyzed demographic, clinical, and serotype data for infants aged 1–90 days. The pre– and post–vaccine introduction periods spanned 1997–2000 and 2001–2010, respectively. RESULTS: Of 513 children with IPD, 36 were 1 to 90 days and accounted for 7% of IPD cases in both the pre– and post–vaccine introduction period. The pre–vaccine IPD incidence rate was 5.0 per 100 000 live births, and was unchanged in the post–vaccine introduction period. IPD caused by PCV7 serotypes decreased by 74% (from 2.2 to 0.58 per 100?000), whereas non-vaccine serotype IPD increased by 57% (from 2.8 to 4.4 per 100 000). Sixteen infants (44%) required intensive care, and 3 (8%) died. Bacteremia without focus (56%) and meningitis (44%) were the predominant syndromes in the pre– and post–vaccine introduction periods, respectively. In the post–vaccine introduction period, serotype 7F was the most common serotype among infants and was responsible for 50% of meningitis. CONCLUSIONS: The incidence of IPD in Utah infants aged 1 to 90 days caused by PCV7 serotypes decreased after PCV7 introduction, but overall incidence was unchanged. In the post–vaccine introduction period, serotype 7F predominated in this age group and was associated with meningitis. PMID:23733800

  10. Family Science Day

    ERIC Educational Resources Information Center

    McCubbins, Sara; Thomas, Bethany; Vetere, Michael

    2014-01-01

    This article describes a family-friendly science day event that encourages scientific discovery through hands-on activities, while also providing an opportunity to learn about scientific careers from actual research scientists and science educators, thereby raising awareness of the importance of STEM in our society. The one-day event bought…

  11. Science Challenge Day

    ERIC Educational Resources Information Center

    Siegel, Deborah

    2013-01-01

    Science fairs can be good motivators, but as extracurricular activities, they leave some students behind. However, by staging a Science Challenge Day at school, educators can involve all students in doing everything from choosing activities to judging projects. This article presents a model for running a successful Science Challenge Day. The…

  12. The Presidents' Day Game

    ERIC Educational Resources Information Center

    Maxwell, D. Jackson

    2008-01-01

    The history behind the holiday commonly called "Presidents' Day" is a bit confusing. It started as a federal holiday called Washington's Birthday. It was a day set aside to honor George Washington for his accomplishments as a founding father of the country. Later, many northern states began to recognize Abraham Lincoln's Birthday as well for his…

  13. Electrochemical Periodicities

    Microsoft Academic Search

    J. A. V. Butler; G. Armstrong

    1932-01-01

    IN the course of experiments on the anodic polarisation of platinum electrodes in dilute sulphuric acid solutions saturated with hydrogen, we have observed, at small current densities, some striking periodicities. The change of potential difference with time in a typical experiment is shown in Fig. 1. In this case the electrode potential falls continuously with a marked break at EH

  14. Group culture of human zygotes is superior to individual culture in terms of blastulation, implantation and life birth

    Microsoft Academic Search

    T. Ebner; O. Shebl; M. Moser; R. B. Mayer; W. Arzt; G. Tews

    2010-01-01

    This prospective study tested a new type of culture dish for the effects of individual culture and autotrophic factors. Within a 6-month period, 72 patients with nine or more fertilized eggs were enrolled in this prospective evaluation. Their 936 zygotes were split into three subgroups (individual culture, individual culture with contact to neighbours, group culture). All concepti were cultured in

  15. Long term organ culture of human prostate tissue in a NASA-designed rotating wall bioreactor

    NASA Technical Reports Server (NTRS)

    Margolis, L.; Hatfill, S.; Chuaqui, R.; Vocke, C.; Emmert-Buck, M.; Linehan, W. M.; Duray, P. H.

    1999-01-01

    PURPOSE: To maintain ex vivo integral prostatic tissue including intact stromal and ductal elements using the NASA-designed Rotating Wall Vessel (RWV) which maintains colocalized cells in an environment that promotes both three-dimensional cellular interactions together with the uniform mass transfer of nutrients and metabolic wastes. MATERIALS AND METHODS: Samples of normal prostate were obtained as a byproduct of transurethral prostatectomy or needle biopsy. Prostatic tissue dissected into small 1 x 1 mm. blocks was cultured in the Rotating Wall Vessel (RWV) Bioreactor for various time periods and analyzed using histological, immunochemical, and total cell RNA assays. RESULTS: We report the long term maintenance of benign explanted human prostate tissue grown in simple culture medium, under the simulated microgravity conditions afforded by the RWV bioreactor. Mesenchymal stromal elements including blood vessels and architecturally preserved tubuloglandular acini were maintained for a minimum of 28 days. Cytokeratins, vimentin and TGF-beta2 receptor and ligand were preserved through the entire culture period as revealed by immunocytochemistry. Prostatic acid phosphatase (PAP) was continuously expressed during the culture period, although somewhat decreased. Prostatic specific antigen (PSA) and its transcript were down regulated over time of culture. Prostatic carcinoma cells from the TSU cell line were able to invade RWV-cultured benign prostate tissue explants. CONCLUSIONS: The RWV bioreactor represents an additional new technology for culturing prostate tissue for further investigations concerning the basic physiology and pathobiology of this clinically important tissue.

  16. Characterization of DNA in cell culture supernatant by fluorescence-detection size-exclusion chromatography.

    PubMed

    Tan, Lihan; Yeo, Veronica; Yang, Yuansheng; Gagnon, Pete

    2015-05-01

    A fluorescence-detection size-exclusion chromatography (FSEC) method was developed to characterize DNA in cell culture supernatant. Samples stained with Picogreen were fractionated by size-exclusion chromatography (SEC) and monitored simultaneously by UV absorbance and fluorescence. SEC provided a size-characterization capability absent from bulk fluorescent assays, and was also free from interference from other fluorescent and UV-absorbing small-molecule cell culture components. FSEC revealed that DNA in mammalian cell culture supernatant exists mostly in the form of nucleosomal arrays. FSEC combined with agarose electrophoresis revealed spontaneous degradation of DNA in mammalian cell culture supernatant over a 30 day period at 4 °C: from arrays containing up to ~40 nucleosomes, down to arrays containing three or fewer nucleosomes. It also detected nucleosomal DNA in wheat, soy, and yeast hydrolysates commonly used to enhance cell culture productivity. PMID:25821116

  17. National Lab Day

    NSDL National Science Digital Library

    NSF joins other partners in National Lab Day, an initiative connecting science, technology, engineering and mathematics (STEM) teachers with STEM professionals who volunteer to share their expertise--and their passion--for science and engineering with students. You can view video clips that explain the basics of what National Lab Day is and how it works, get tips from a professional educator about how to effectively help in the classroom and learn about some NSF-funded projects that already are conducting activities in the spirit of Lab Day.

  18. Periodic Monopoles

    E-print Network

    R. S. Ward

    2005-12-28

    This paper deals with static BPS monopoles in three dimensions which are periodic either in one direction (monopole chains) or two directions (monopole sheets). The Nahm construction of the simplest monopole chain is implemented numerically, and the resulting family of solutions described. For monopole sheets, the Nahm transform in the U(1) case is computed explicitly, and this leads to a description of the SU(2) monopole sheet which arises as a deformation of the embedded U(1) solution.

  19. Day Traders At Risk

    NSDL National Science Digital Library

    Missner, Emily D.

    On July 29, 1999, Mark Barton, a 44-year old day trader, opened fire at two Atlanta brokerage firms, killing nine people and injuring thirteen before taking his own life. Barton had recently lost an estimated half a million dollars trading, according to The New York Times. Coincidentally, just a week and a half after the tragedy in Atlanta, the North American Securities Administrators Association (NASAA) released a study condemning the practice of day trading, finding that, of the 4,093 trades in 26 accounts analyzed, "seventy percent of the accounts lost money and were traded in a manner that realized a 100% Risk of Ruin (loss of all funds)." The study concluded, "It is absolutely clear that the average public investor should refrain from short-term trading." This week's In the News looks at the day trading industry and its effectiveness. The eight resources listed provide studies, recent news, background information, and commentary on day trading.

  20. Volunteer Day Student Voices

    E-print Network

    Pilyugin, Sergei S.

    Orange Creek: Help us clean and keep Little Orange Creek beautiful. Learn about plants, animals, weeds in playing music and have an instrument, please sign up. Let's make this a memorable day! Meet at the Norman

  1. American Experience: Earth Days

    NSDL National Science Digital Library

    In late 1969, Wisconsin Senator Gaylord Nelson hired Denis Hayes to organize a national teach-in day about the importance of protecting the environment for future generations. On April 22, 1970, the first Earth Day was held and over 20 million Americans participated via a variety of celebrations and demonstrations. This website is designed to be a companion to the American Experience documentary on the history of Earth Day, and it includes a teacher's guide, several special interviews, and the documentary in its entirety. The film was directed by Robert Stone, and it serves as "a poetic meditation on man's complex relationship with nature and an engaging history...of groundbreaking eco-activism." On the homepage, visitors should also take note of the photo gallery, the interactive timeline of the modern environmental movement, and a telling interview with the father of the "Green Revolution", Norman Borlaug. Visitors are also encouraged to share their own Earth Day memories on the site.

  2. Make a Splash Day

    NSDL National Science Digital Library

    April Rust

    2004-07-01

    Project WET's Make a Splash Water Education Days provide plentiful learning opportunities for elementary students and preservice teachers. At these annual, all-day events held in nearly every state across the country each September, students participate in interactive activities and exhibits to learn about water resources and explore how human behaviors, such as development and recreation, can affect the quality of our country's waterways.

  3. Scientist of the Day

    NSDL National Science Digital Library

    Maria Salinas

    2005-10-01

    A first grade teacher in an urban school, eager to bring authentic science into the classroom, provides an opportunity for her students to experience science adventures and explorations, while also getting parents involved. She implemented a program called Scientist of the Day which allows students to experience simple hands-on science experiments, and to involve their parents both in and out of the classroom. The idea is for every child to have a turn being "Scientist of the Day".

  4. Trading volume around ex-dividend days

    Microsoft Academic Search

    Rakesh Bali; Jack C. Francis

    2011-01-01

    We tested how ex-day trading volume is impacted by the reduction in capital gains tax rate to 20% (May 1997) and the move to trading in 6.25¢ price increments (June 1997). We found excess trading volume around the ex-days in the pre- and post-event periods for both high- and low-yield groups. The abnormal volume falls significantly after the move to

  5. Diterpenoid production in hairy root culture of Salvia sclarea L.

    PubMed

    Ku?ma, Lukasz; Bruchajzer, Elzbieta; Wysoki?ska, Halina

    2008-01-01

    Growth and diterpenoid accumulation (salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone) during the growth cycle of a Salvia sclarea hairy root culture are described. The roots transformed by Agrobacterium rhizogenes (LBA 9402) were cultured in half-strength B5 liquid medium supplemented with 30 g L(-1) sucrose under light (16 h/8 h light/dark). A culture period of 30 days was optimal for both biomass and diterpenoid production. The total content of four diterpenoids in the hairy roots [(27.3 +/- 0.6) mg g(-1) dry weight] was higher than that of roots of field-grown S. sclarea plants [(3.15 +/- 0.15) mg g(-1) dry weight]. In transformed roots, aethiopinone was the main diterpenoid, whereas the principal diterpenoid of natural roots was salvipisone. PMID:18811013

  6. Chinese Business Practices and Culture

    E-print Network

    Hickman, Mark

    and culture, interact with Chinese students and businesses followed by a 5 day business visit to internationalMGMT228 Chinese Business Practices and Culture School of Business and Economics 2014business Costs information on the course, or to apply: email: jeremy.orourke@canterbury.ac.nz web: www.bsec.canterbury.ac.nz/international-business

  7. Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow.

    PubMed

    Sipaúba-Tavares, L H; Millan, R N; Berchielli-Morais, F A

    2013-08-01

    Growth rate and medium parameters between two bench scale volumes (13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of medium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 10(5) cells.mL(-1) on the 12th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water culture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions. PMID:24212699

  8. 3D Hepatic Cultures Simultaneously Maintain Primary Hepatocyte and Liver Sinusoidal Endothelial Cell Phenotypes

    PubMed Central

    Kim, Yeonhee; Rajagopalan, Padmavathy

    2010-01-01

    Developing in vitro engineered hepatic tissues that exhibit stable phenotype is a major challenge in the field of hepatic tissue engineering. However, the rapid dedifferentiation of hepatic parenchymal (hepatocytes) and non-parenchymal (liver sinusoidal endothelial, LSEC) cell types when removed from their natural environment in vivo remains a major obstacle. The primary goal of this study was to demonstrate that hepatic cells cultured in layered architectures could preserve or potentially enhance liver-specific behavior of both cell types. Primary rat hepatocytes and rat LSECs (rLSECs) were cultured in a layered three-dimensional (3D) configuration. The cell layers were separated by a chitosan-hyaluronic acid polyelectrolyte multilayer (PEM), which served to mimic the Space of Disse. Hepatocytes and rLSECs exhibited several key phenotypic characteristics over a twelve day culture period. Immunostaining for the sinusoidal endothelial 1 antibody (SE-1) demonstrated that rLSECs cultured in the 3D hepatic model maintained this unique feature over twelve days. In contrast, rLSECs cultured in monolayers lost their phenotype within three days. The unique stratified structure of the 3D culture resulted in enhanced heterotypic cell-cell interactions, which led to improvements in hepatocyte functions. Albumin production increased three to six fold in the rLSEC-PEM-Hepatocyte cultures. Only rLSEC-PEM-Hepatocyte cultures exhibited increasing CYP1A1/2 and CYP3A activity. Well-defined bile canaliculi were observed only in the rLSEC-PEM-Hepatocyte cultures. Together, these data suggest that rLSEC-PEM-Hepatocyte cultures are highly suitable models to monitor the transformation of toxins in the liver and their transport out of this organ. In summary, these results indicate that the layered rLSEC-PEM-hepatocyte model, which recapitulates key features of hepatic sinusoids, is a potentially powerful medium for obtaining comprehensive knowledge on liver metabolism, detoxification and signaling pathways in vitro. PMID:21103392

  9. [The effect of biotic elicitors on the production of flavonoids in suspension cultures of Bellis perennis L].

    PubMed

    Siatka, T; Kasparová, M

    1999-11-01

    The effect of two biotic elicitors (homogenates of Pseudomonas aeruginosa and Candida utilis) in a suspension culture of Bellis perennis L. cultivated in the dark and day light period were examined. The growth of the culture was not negatively affected by the action of elicitors. Increased production of flavonoids in the culture depended on the sort, concentration and space of time of action of the elicitor, and on the manner of cultivation from the viewpoint of the light regimen. The best results were achieved with the use of the homogenate of Candida utilis after 24 hours in cultivation under the day light period and after 48 hours in cultivation in the dark. PMID:10748744

  10. Expression of the capacity to release (/sup 3/H)norepinephrine by neural crest cultures

    SciTech Connect

    Maxwell, G.D.; Sietz, P.D.

    1983-09-01

    Cultures of trunk neural crest cells from quail embryos were tested for their ability to release (/sup 3/H)norepinephrine ((/sup 3/H)NE) in response to depolarization. After 7 days in vitro, exposure of the cultures to either the alkaloid veratridine or 40 mM K+ results in the evoked release of (/sup 3/H)NE. The release evoked by veratridine is blocked in the presence of tetrodotoxin. The release evoked by increased K+ is blocked by the calcium antagonist cobalt. Release in response to the nicotinic cholinergic agonist 1,1-dimethyl-4-phenylpiperazine was also observed. The amount of evoked release is highly correlated with the number of histochemically demonstrable catecholamine-containing cells in a given culture. Autoradiography reveals that the radioactivity taken up by these cultures is located in a subpopulation of cells whose morphology resembles that of the histochemically detectable catecholamine-containing cell population. Whereas capacity for the release of (/sup 3/H) NE is readily detectable after 7 days in vitro, it is detectable only with difficulty after 4 days in vitro. There is a greater than 6-fold increase in uptake capacity over the period of 4 to 7 days in vitro. These results demonstrate that neural crest cultures grown without their normal synaptic inputs or targets can exhibit the capacity for stimulus secretion coupling characteristic of synaptic neurotransmitter release.

  11. Chambers's Book of Days

    NSDL National Science Digital Library

    Subtitled A Miscellany of Popular Antiquities in connection with the Calendar, Chambers's Book of Days is essentially a collection of "On this Day" trivia, short pieces, and other interesting tidbits, including history, literature, biography, and "oddities of human life and character." Digitized by the University of Wisconsin-Madison Libraries, this electronic reprint may be browsed in page order or via a calendar navigator. The site offers a fun look into pop-history/ tabloid news of the late nineteenth century. For instance, I discovered that on the day of my birth in 1626 "a cod-fish was brought to Cambridge market, which upon being opened, was found to contain a book in its maw or stomach." An auspicious date to be born indeed.

  12. Australia National Sorry Day

    NSDL National Science Digital Library

    1998-01-01

    "Sorry Day," held on May 26, 1998 in Australia, was an attempt on the part of some Australians to come to grips with the policy of forced removal of Aboriginal children that took place for 150 years until the 1970s. The National Sorry Day site is provided by the Reconciliation and Social Justice Project of the Australasian Legal Information Institute. The site contains background and an educational activities kit. The centerpiece, however, is full text access (in the Publications) to Bringing Them Home: Report of the National Inquiry into the Separation of Aboriginal and Torres Strait Islander Children from Their Families, which was tabled by the Australian Federal Parliament exactly one year before Sorry Day.

  13. Earth Day Network

    NSDL National Science Digital Library

    Every spring, get ready for Earth Day with a visit to the official Web site of the Earth Day Network Web site, "an alliance of 5,000 groups in 184 countries working to promote a healthy environment and a peaceful, just, sustainable world." The Web site contains a wealth of information and resources designed to increase public awareness of important environmental issues and provide opportunities for individuals and communities to get involved. Visitors may read about current Earth Day Network campaigns and programs, as well as review environmental issues and threats with a collection of fact sheets and other resources. In all, this comprehensive and well-organized site is definitely worth a visit for anyone who would like to learn more about environmental problems and environmental activism.

  14. Midterm periods of solar filaments

    NASA Astrophysics Data System (ADS)

    Zou, Peng; Li, Qixiu

    2014-12-01

    On the basis of the Carte Synoptique catalogue of solar filaments from March 1919 to December 1989, we measure power spectra of detrended full-disk (FSFNs, latitudinal bands: 0?-90?), low-latitude (LSFNs, latitudinal bands: <50?), and high-latitude (HSFNs, latitudinal bands: ?50?) solar filament numbers by Maximum Entropy Method (MEM) and Continuous wavelet transform to detect midterm periods. It is found as the following: (1) FSFNs and LSFNs have the same midterm periodicity, while HSFNs show a different midrange periodicity. Some periods frequently mentioned in other solar indices are also detected from the solar filament numbers, such as 2-3 year period (quasi-biennial oscillation—QBO), ˜1.7 year, ˜1.3 year, ˜1 year, 150-157 day period (Rieger period), and 6.0-6.4 months (Rieger-type period). These periods are intermittent during considered time span. Some of them are missing in some solar cycles. (2) QBO is detected from total data and most solar cycles of FSFNs, LSFNs, and HSFNs. It may be related to oscillation of magnetic field of solar surface. (3) Approximately 1.3 year period occasionally appears, but ˜1.7 year period is hardly seen. These two periods probably are seasonal effects. (4) Approximately 1 year period is detected from both total data and every solar cycle of FSFNs and LSFNs but hardly detected from HSFNs. It is perhaps connected with sunspot activity. (5) Rieger period of 5.0-5.2 months is detected in total data and even solar cycles of HSFNs. Rieger-type period of 6.0-6.4 months is found in total data and most solar cycles, except cycle 18 of LSFNs and FSFNs. These periods seem to be subharmonics of ˜11 year period.

  15. Length of the Day

    NSDL National Science Digital Library

    2012-08-03

    In this activity, students measure the length of the day using the rotation of the Earth, and discover that the Sun is not exactly in the same place at the same clock time every day, understand that the changes are due to motions of the Earth, and lead to differences in solar, star, and sidereal time. This resource is from PUMAS - Practical Uses of Math and Science - a collection of brief examples created by scientists and engineers showing how math and science topics taught in K-12 classes have real world applications.

  16. Epidemiology of Viral Infections and Evaluation of the Potential Benefit of OM85 BV on the Virologie Status of Children Attending Day-Care Centers

    Microsoft Academic Search

    M. Aymard; J. J. Chomel; J. P. Allard; D. Thouvenot; D. Honegger; D. Floret; J.-P. Boissel; J.-P. Collet; F. Dürr; J. Gillet; N. Bossard; L. Lyon

    1994-01-01

    Viral investigations were performed during 4 winter seasons (88\\/89, 89\\/90, 92\\/93, 93\\/94) in children attending day-care centers (DCCs) in the Rhone Département in eastern France. Over the total observation period of 4 winter seasons, 780 children were screened with a nasal swab for the presence of viruses. Of those, 230 (29.5%) had a positive viral culture. The viruses identified were

  17. Cultural Neuroscience

    PubMed Central

    Ames, Daniel L.; Fiske, Susan T.

    2013-01-01

    Cultural neuroscience issues from the apparently incompatible combination of neuroscience and cultural psychology. A brief literature sampling suggests, instead, several preliminary topics that demonstrate proof of possibilities: cultural differences in both lower-level processes (e.g. perception, number representation) and higher-order processes (e.g. inferring others’ emotions, contemplating the self) are beginning to shed new light on both culture and cognition. Candidates for future cultural neuroscience research include cultural variations in the default (resting) network, which may be social; regulation and inhibition of feelings, thoughts, and actions; prejudice and dehumanization; and neural signatures of fundamental warmth and competence judgments. PMID:23874143

  18. Obtaining Synchronous Cultures of Algae

    Microsoft Academic Search

    W. A. Glooschenko; H. Curl

    1968-01-01

    A COMMUNICATION from Lafeber and Steenbergen1 described a ``simple device for obtaining synchronous cultures of algae''. They stated that Scenedesmus obliquus, Ankistrodesmus falcatus, and Chlorella vulgaris all underwent synchronous cell division induced by the photo-period used.

  19. Preparation of Triple-Negative Breast Cancer Vaccine through Electrofusion with Day-3 Dendritic Cells

    PubMed Central

    Li, Xi; Liu, Ruilei; Jiang, Hua; Huang, Zenan; Liu, Yu; Wu, Juekun; Huang, Yong

    2014-01-01

    Dendritic cells (DCs) are professional antigen-presenting cells (APCs) in human immune system. DC-based tumor vaccine has met with some success in specific malignancies, inclusive of breast cancer. In this study, we electrofused MDA-MB-231 breast cancer cell line with day-3 DCs derived from peripheral blood monocytes, and explored the biological characteristics of fusion vaccine and its anti-tumor effects in vitro. Day-3 mature DCs were generated from day-2 immature DCs by adding cocktails composed of TNF-?, IL-1?, IL-6 and PEG2. Day-3 mature DCs were identified and electofused with breast cancer cells to generate fusion vaccine. Phenotype of fusion cells were identified by fluorescence microscope and flow cytometer. The fusion vaccine was evaluated for T cell proliferation, secretion of IL-12 and IFN-?, and induction of tumor-specific CTL response. Despite differences in morphology, day-3 and day-7 DC expressed similar surface markers. The secretion of IL-12 and IFN-? in fusion vaccine group was much higher than that in the control group. Compared with control group, DC-tumor fusion vaccine could better stimulate the proliferation of allogeneic T lymphocytes and kill more breast cancer cells (MDA-MB-231) in vitro. Day-3 DCs had the same function as the day-7 DCs, but with a shorter culture period. Our findings suggested that day-3 DCs fused with whole apoptotic breast cancer cells could elicit effective specific antitumor T cell responses in vitro and may be developed into a prospective candidate for adoptivet immunotherapy. PMID:25036145

  20. Subacute (90 days) oral toxicity studies of Kombucha tea.

    PubMed

    Vijayaraghavan, R; Singh, M; Rao, P V; Bhattacharya, R; Kumar, P; Sugendran, K; Kumar, O; Pant, S C; Singh, R

    2000-12-01

    Kombucha tea (KT) is a popular health beverage and is used as an alternative therapy. KT is prepared by placing the kombucha culture in solution of tea and sugar and allowing to ferment. The inoculum is a fungus consisting of symbiotic colony of yeast and bacteria. KT is consumed in several countries and is believed to have prophylactic and therapeutic benefits in a wide variety of ailments, viz., intestinal disorders, arthritis, ageing and stimulation of immunological system. Though KT is used in several parts of the world its beneficial effects and adverse effects have not been scientifically evaluated. Since there are no animal toxicological data on KT, subacute oral toxicity study was carried out. Five groups of rats were maintained: (a) control group given tap water orally, (b) KT given 2 ml/kg orally, (c) plain tea (PT) given 2 ml/kg orally, (d) KT given in drinking water, 1% (v/v) and (e) PT given in drinking water, 1% (v/v). The rats were given this treatment daily for a period of 90 days. Weekly records of weight, feed intake, water intake and general behaviour were monitored. There was no significant difference in the growth of the animals as evidenced by the progressive body weight change. The organ to body weight ratio and histological evaluation did not show any toxic signs. The haematological and biochemical variables were within the clinical limits. The study indicates that rats fed KT for 90 days showed no toxic effects. PMID:11351863

  1. Time-dependent changes in hemocytes of eastern oysters, Crassostrea virginica, and northern bay scallops, Argopecten irradians irradians, exposed to a cultured strain of Prorocentrum minimum

    Microsoft Academic Search

    Hélène Hégaret; Gary H. Wikfors

    2005-01-01

    Juvenile oysters and scallops were exposed, under controlled laboratory conditions, to bloom concentrations of a cultured dinoflagellate with demonstrated lethal and sub-lethal, pathological effects upon these bivalves. Immune status of the mollusks was assessed periodically, using flow-cytometric hemocyte analyses for hematological characteristics and several hemocyte functions, during 7 days of continuous exposure. Principal Components Analysis (PCA) was used to develop

  2. Income per day

    NSDL National Science Digital Library

    N/A N/A (None; )

    2006-09-13

    The number of people living on less than one dollar per day has decreased in some places in the world while it has stayed steady in other areas. This could be because the population has been rising in those areas and the economy cannot cope in those certain places.

  3. One Play a Day

    ERIC Educational Resources Information Center

    Blankenship, Mark

    2007-01-01

    Undergraduate theater students rarely get the chance to work on a major world premiere, but this year hundreds of them will. Currently, more than 70 colleges and universities are participating in "365 Days/365 Plays," an ambitious project from Pulitzer Prize-winning playwright Suzan-Lori Parks. Every week, as they mount their portion of this epic…

  4. Seize the Day

    ERIC Educational Resources Information Center

    Berkey, Tim

    2008-01-01

    In order to improve what happens in classrooms, a considerable amount of work needs to take place between teachers and principals. This can only happen if campus leaders make dramatic shifts in how and where they spend their daily time. Principals can have a greater impact on teaching and learning by transforming their work one day at a time. The…

  5. Shady Day MEA

    NSDL National Science Digital Library

    Kerri Caudill

    2012-07-31

    This Model Eliciting Activity (MEA) is written at a 5th grade level. The Shady Day MEA provides students with an engineering problem in which they must work as a team to design a procedure to select the best beach umbrella for certain situations.

  6. First Day of School

    ERIC Educational Resources Information Center

    Bort, Nancy

    2004-01-01

    In this brief article, the author, a science teacher at F. C. Hammond Middle School in Alexandria, Virginia, describes how the setting up of a simple science experiment on the first day of school can get students excited about learning science. The experiment involves heating a small amount of water in a flask, then covering the opening of the…

  7. International Children's Book Day

    E-print Network

    Watson, Craig A.

    International Children's Book Day April 2nd 2013 Folktales Storytelling & wORKSHOP By Barry Stewart, Lobby 2pm ­ 3 pm: UF Smathers Library, Room 1A Books with WingsBy Peter Sís 7pm ­ 9pm: PK Yonge Performing Arts Center 1080 SW 11th Street, Gainesville Peter Sís Award Winning Children's Book Author

  8. Nutrition on match day

    Microsoft Academic Search

    Clyde Williams; Luis Serratosa

    2006-01-01

    What players should eat on match day is a frequently asked question in sports nutrition. The recommendation from the available evidence is that players should eat a high-carbohydrate meal about 3 h before the match. This may be breakfast when the matches are played around midday, lunch for late afternoon matches, and an early dinner when matches are played late in

  9. Sunny Day Painting

    NSDL National Science Digital Library

    Kohl Children's Museum

    2012-01-01

    In this activity, learners explore properties of water and watch evaporation happen by "painting" with water in the sun. On a sunny day, learners use a variety of painting tools (brushes, rollers, spray bottles, etc.) to "paint" water on sidewalks, buildings, fences, etc. outside. Learners observe how the water moves and evaporates from the surfaces.

  10. Sun-Earth Day

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Michael Sandras, a member of the Pontchartrain Astronomical Society, explains his solar telescope to students of Second Street in Bay St. Louis, Hancock County and Nicholson elementary schools in StenniSphere's Millennium Hall on April 10. The students participated in several hands-on activities at Stennis Space Center's Sun-Earth Day celebration.

  11. Macy's Thanksgiving Day Parade

    NSDL National Science Digital Library

    Users taking a trip to watch the Macy's Thanksgiving Day Parade or maybe just planning to watch it from home may enjoy the parade site from NYCTourist. It features news on the parade, photos of balloons and the crowd from past parades, and a brief review of the parade's history with several historic photos.

  12. Foundation Day Meeting

    E-print Network

    Hacker, Randi; Tsutsui, William; Haufler, Marsha

    2007-11-28

    War occurred on National Foundation Day? Hard to say but it must have pleased Tangun who founded Korea 4,340 years ago. Tangun is believed to be the son of a god and a woman transformed from a bear and is said to have established his kingdom...

  13. Welcome to Rock Day

    NSDL National Science Digital Library

    Jeaneen Benhart

    2004-01-01

    Seeking to revitalize a unit on rocks, sand, and soil for first-graders, the authors created new hands-on lessons. These included testing the hardness of rocks, making models of the Earth, and sorting rocks. As a culminating activity, students participated in a series of Rock Day events that focused on the three different types of rocks and the rock cycle.

  14. We Love Science Day.

    ERIC Educational Resources Information Center

    Kepler, Lynne

    1986-01-01

    Describes the goals and outcomes of the "We Love Science Day" programs that resulted from the inservice course, "Creative Integration of Science in Elementary Education" for Pennsylvania teachers. Provides samples of the hands-on activities that were offered to students, parents, and teachers. Includes a calendar of extracurricular science…

  15. NetDay

    NSDL National Science Digital Library

    The mission of NetDay is "to connect every child to a brighter future by helping educators meet educational goals through the effective use of technology." The nonprofit organization provides direct service to 25 schools in five underserved communities and offers resources, guidance and skilled people to assist with technology integration in schools. The website posts issue articles, resource links and guides and serves as a forum to raise national awareness on topics related to the Internet. The organization's free online publication, NetDayCompass, includes more than 2,000 education technology resources for K-12 decision-makers. The site also features results from research, surveys, and analysis that NetDay conducts on issues that matter to the targeted communities. In 2003, the organization launched Speak Up Day, which is intended to "give a voice to all stakeholders in the educational community and to frame a new conversation around education and technology." Highlights from previous online surveys of teachers and students are posted on the site. Students have until November 12 to register their school and include their voice in the 2004 survey.

  16. Cultural Proficiency

    ERIC Educational Resources Information Center

    Guerra, Patricia L.; Nelson, Sarah W.

    2007-01-01

    Cultural proficiency is defined as "the policies and practices of an organization or the values and behaviors of an individual that enable the agency or person to interact effectively in a culturally diverse environment." The diverse composition of today's classrooms demands that schools and educators be culturally proficient, yet few of them are.…

  17. Day One Sustainability

    ERIC Educational Resources Information Center

    Orr, John; Ibell, Timothy; Evernden, Mark; Darby, Antony

    2015-01-01

    Emissions reductions targets for the UK set out in the Climate Change Act for the period to 2050 will only be achieved with significant changes to the built environment, which is currently estimated to account for 50% of the UK's carbon emissions. The socio-technological nature of Civil Engineering means that this field is uniquely placed to lead…

  18. President Clinton: Final Days

    NSDL National Science Digital Library

    Available at Pocketmovies.net, this six-minute video is a humorous and clever peek into the twilight days at the Clinton White House. Shot for the Annual White House Correspondents's Dinner, the "home movie" depicts a lonely President Clinton taking care of the yard work, doing the laundry, and other mundane tasks. The movie is an entertaining diversion and demonstrates that even the "leader of the free world" can have a (self-deprecating) sense of humor.

  19. Flight Day 2 Highlights

    NASA Technical Reports Server (NTRS)

    2003-01-01

    The STS-107 second flight day begins with a shot of the Spacehab Research Double Module. Live presentations of experiments underway inside of the Spacehab Module are presented. Six experiments are shown. As part of the Space Technology and Research Student Payload, students from Australia, China, Israel, Japan, New York, and Liechtenstein are studying the effect that microgravity has on ants, spiders, silkworms, fish, bees, granular materials, and crystals. Mission Specialist Kalpana Chawla is seen working with the zeolite crystal growth experiment.

  20. Modern-day prospecting

    SciTech Connect

    Not Available

    1984-12-01

    Locating productive oil and mineral deposits takes a combination of money, time, and luck. However, the modern-day prospector has several new tools at his disposal. Some are conventional and relatively inexpensive; others are more innovative and high-priced. Improved seismic techniques, image processing, and some other innovative prospecting methods are described. These techniques are helping oil companies improve their exploration win-loss records.

  1. Production, maintenance and plant regeneration from cell suspension cultures of Stevia rebaudiana (Bert.) Bertoni

    Microsoft Academic Search

    C. M. Ferreira; Walter Handro

    1988-01-01

    A method is described for producing and maintaining Stevia rebaudiana suspensions and regeneration of plants from calli derived from cell suspensions. Suspension cultures composed of isolated cells (ca. 10%) and cellular aggregates (5–100 cells) were obtained in 20–30 days by using friable callus as the initial inoculum in liquid media with BA (0.5 mg\\/l)+2,4-D (1.0 mg\\/l), and periodic filtering (100–500

  2. Arbor Day Foundation

    NSDL National Science Digital Library

    Arbor Day was first observed in 1872, and the Arbor Day Foundation was founded in 1972 in Nebraska. The Arbor Day website contains a trove of information for visitors to peruse, make plans around, and learn from. The "Trees" section of the website includes "What Tree is That?", an online guide for tree identification; a "Tree Guide", that covers more than 200 different species of trees; and a quiz called "The Right Tree in the Right Place", about how and why to first make a simple plan, considering size and shape, when planting trees around a house. The "Programs" section of the website is an excellent resource for visitors such as teachers, parents and students. "Nature Explore" helps connect kids with nature via the "Resource Guide", which contains "field-tested resources developed to bring nature into children's daily learning in sustainable, significant, positive and joyful ways." Interested visitors can use the link entitled "Request a Resource Guide", to get a free copy. "Nature Explore Classroom Certification" links teachers to the certification process and examples of certified classrooms and classrooms in-progress.

  3. A method for obtaining Schwann cell cultures from adult rabbit nerve based on "in vitro" pre-degeneration and neuregulin treatment.

    PubMed

    de la Fuente, I; Alcalde, I; Gamboa, O L; Garrosa, M; Gayoso, M J

    2012-01-01

    Schwann cells (SCs) are basic elements for cell therapy and tissue engineering in the central and peripheral nervous system. Therefore, the development of a reliable method to obtain SC cultures is required. For possible therapeutic applications the cultures need to produce a sufficiently large number of SCs with a high level of purity in a relatively short period of time. To increase SC yield and purity we pre-degenerated pieces of 1-2 mm of adult rabbit sciatic nerves by incubating them for seven days in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum, penicillin/streptomycin and NRG1-?1. Following pre-degeneration the nerve pieces were dissociated and then cultured for 6 or 15 days in the same culture medium. After 6 days of culture we obtained around 9.5x10³ cells/mg with approximately 94% SCs (S-100 positive) purity. After 15 days of culture the yield was about 80x10³ cells/mg and the purity was approximately 75%. Pre-degeneration and subsequent culture of small pieces of adult nerve with NRG1-?1 supplemented medium increased the number of SCs and restricted the overgrowth of fibroblast-like cells. PMID:22127601

  4. From microcarriers to hydrodynamics: introducing engineering science into animal cell culture.

    PubMed

    Croughan, Matthew S; Hu, Wei-Shou

    2006-10-01

    Professor Daniel I.C. Wang has conducted research in animal cell culture for approximately 40 years. Over that long time period and still to this day, he successfully addresses a multitude of engineering challenges, taking a unique, creative, systems-driven but still fundamental approach. As mammalian cell culture has become the predominant method of manufacturing therapeutic proteins, the impact of his leadership, not only in research but also student recruitment and education, has played a key role in the success of the bio/pharmaceutical industry. PMID:16933297

  5. A sunspot periodicity and the solar rotation

    NASA Technical Reports Server (NTRS)

    Knight, J. W.; Sturrock, P. A.; Schatten, K. H.

    1980-01-01

    A least squares power spectrum analysis of daily sunspot numbers for the last 122 years yielded a statistically significant peak at 12.0715 plus or minus .002 days period. This feature at 11.685 days (sidereal) of the sunspot spectrum is discussed in relation to the peak at 12.22 days (sidereal) which Dicke found in his oblateness data. The data is attributed to the Sun's core if the core rotates at either 12.0715 days or 24.1430 days period (synodic). It is suggested that spacecraft observations combined with correlative analysis of solar surface features between eastern and western hemispheres could further reveal a basic core periodicity. A Dicke type space oblateness experiment is discussed for providing better photospheric observations than a ground instrument to determine the core periodicity.

  6. Probability of Negative Mycobacterium tuberculosis Complex Cultures Based on Time to Detection of Positive Cultures: a Multicenter Evaluation of Commercial-Broth-Based Culture Systems

    PubMed Central

    Budnick, Gary E.; Elliott, Thor; Gillim-Ross, Laura; Hildred, Mary V.; Mahlmeister, Peggy; Parrish, Nicole; Pentella, Michael; Vanneste, Jolene; Wang, Yun F. (Wayne); Starks, Angela M.

    2012-01-01

    We conducted a multicenter study to determine whether Mycobacterium tuberculosis complex (MTBC) cultures in automated broth-based systems could reliably be considered negative sooner than 6 weeks. Laboratory sites used Bactec MGIT or BacT/Alert and tracked results of time to detection of all mycobacteria (TTD-all, n = 1547) and of MTBC (TTD-MTBC, n = 466) over 6-month periods from primarily (93%) respiratory specimens. Cumulative percentages by day detected and median TTD of initial and follow-up specimens were analyzed. The median TTD-MTBC for MGIT (n = 6 sites) was 14 days. For laboratories using standard processing procedures, 100% of MTBC were detected from initial and follow-up specimens in 28 and 35 days, respectively, and no yield of MTBC on solid or MGIT liquid media was observed after 5 weeks. The median TTD-MTBC for BacT/Alert (n = 3 sites) was 18 days, with 95% and 100% detected within 37 and 42 days, respectively. Analysis of TTD of positive MTBC cultures in broth can predict the probability of culture negativity at defined time points. Receipt of interim negative reports earlier than 6 weeks could assist clinicians in considering alternative diagnoses and could alter the timing and prioritization of public health interventions. Laboratories should analyze their own TTD data to inform protocol decisions. Laboratories using MGIT could issue reports of no growth of MTBC on initial specimens as early as 4 weeks and for patients undergoing treatment as early as 5 weeks postinoculation. PMID:22837326

  7. A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2

    PubMed Central

    Martin, Matthew R.; Fornero, Jeffrey J.; Angenent, Largus T.

    2013-01-01

    We optimized and tested a postbioprocessing step with a single-culture archaeon to upgrade biogas (i.e., increase methane content) from anaerobic digesters via conversion of CO2 into CH4 by feeding H2 gas. We optimized a culture of the thermophilic methanogen Methanothermobacter thermautotrophicus using: (1) a synthetic H2/CO2 mixture; (2) the same mixture with pressurization; (3) a synthetic biogas with different CH4 contents and H2; and (4) an industrial, untreated biogas and H2. A laboratory culture with a robust growth (dry weight of 6.4–7.4?g/L; OD600 of 13.6–15.4), a volumetric methane production rate of 21?L/L culture-day, and a H2 conversion efficiency of 89% was moved to an industrial anaerobic digester facility, where it was restarted and fed untreated biogas with a methane content of ~70% at a rate such that CO2 was in excess of the stoichiometric requirements in relation to H2. Over an 8-day operating period, the dry weight of the culture initially decreased slightly before stabilizing at an elevated level of ~8?g/L to achieve a volumetric methane production rate of 21?L/L culture-day and a H2 conversion efficiency of 62%. While some microbial contamination of the culture was observed via microscopy, it did not affect the methane production rate of the culture. PMID:24194675

  8. Noon Day Project

    NSDL National Science Digital Library

    Stevens Institute of Technology

    2009-01-01

    This webpage is about the Noon Day project, which is a worldwide effort to measure the circumference of the earth. Students try to measure by a method created 2000 years ago first used by Eratosthenes. Students at various sites around the world will measure shadows cast by a meter stick and compare their results. From this data students will be able to calculate the circumference of the earth. There is an informational video explaining the project. Included on the webpage is a photo gallery, schedule, lesson plans, dissuction area, glossary, and archive.

  9. 6.EE Busy Day

    NSDL National Science Digital Library

    This is a task from the Illustrative Mathematics website that is one part of a complete illustration of the standard to which it is aligned. Each task has at least one solution and some commentary that addresses important asects of the task and its potential use. Here are the first few lines of the commentary for this task: The Jonas family had a really busy day. After leaving their home, Mrs. Jonas dropped son Cody at his tennis practice. She then drove daughter Kristin t...

  10. 3.NF Snow Day

    NSDL National Science Digital Library

    This is a task from the Illustrative Mathematics website that is one part of a complete illustration of the standard to which it is aligned. Each task has at least one solution and some commentary that addresses important asects of the task and its potential use. Here are the first few lines of the commentary for this task: Alec and Felix are brothers who go to different schools. The school day is just as long at Felix' school as at Alec's school. At Felix' school, there a...

  11. Aquarist for a Day

    NSDL National Science Digital Library

    Jonathan Bird Productions

    2007-08-26

    In this video Jonathan visits the New England Aquarium as a volunteer aquarist for a day to learn what it takes to care for thousands of fish in dozens of exhibits, up to the massive 200,000 gallon Giant Ocean Tank (GOT). What he finds is an eye opener—it’s not all fun and games. Maintaining an aquarium is serious work. But it does have its benefits, as Jonathan discovers while diving in the GOT and feeding the sharks. This segment won a New England Emmy Award! Please see the accompanying study guide for educational objectives and discussion points.

  12. One cold autumn day.

    PubMed

    de Schweinitz, Peter

    2015-05-01

    Behavioral change is at the heart of effective primary care, but when patients don't change, how do we account for our days? In this personal essay, I relate an encounter with a patient who wants to quit smoking, lose weight, and control her diabetes. I am discouraged when she deflects my recommendations, but a colleague's comment encourages a deeper inquiry. Knowing the patient's story and deepening the conversation, however, do not guarantee change. The experience reminds me why patience, humility, and faith are core values of the primary care physician. PMID:25964410

  13. International Women's Day Celebrated

    NSDL National Science Digital Library

    With historical origins dating back to the early 20th century, International Women's Day has been officially celebrated since 1977, when the United Nations made a special declaration setting the date in early March every year. This year saw a flurry of important events around the world that coincided with March 8th, including ceremonies designed to honor women of national and international importance in many countries and a number of valuable statements made on behalf regarding the cause of gender equality. A number of prominent women also gave speeches this Monday, including Nobel Peace Prize laureate Shirin Ebadi, who noted that "The rights of women and democracy are one and the same." A study released this past Monday by the Inter-Parliamentary Union noted that women make up a record-breaking 15.3 percent of the world's lawmakers. The country with the greatest percentage of female lawmakers is Rwanda (49 percent), followed closely by Sweden, with 45 percent. While there was much to celebrate, there were also some ominous trends noted by lawmakers and officials, including the rapid and disturbing increase of young women with HIV around the world in the past few years, a fact noted by UN Secretary General Kofi Annan during his remarks to a special UN session.The first site will take visitors to a news piece from the Baltimore Sun that contains Shirin Ebadi's remarks made on International Women's Day. The second link leads to a news piece from Al-Jazeera that discusses the rapid increase of HIV among young women, a public health issue that is becoming endemic in certain parts of the developing world. The third link leads to an article from the Cornell (University) Daily Sun that offers details on a celebration honoring women on their campus who had worked to improve the situation of women locally, nationally, and internationally. The fourth link will take visitors to the rather comprehensive website for International Women's Day 2004 created by the United Nations. The site contains information on the events sponsored by the UN and its Women Watch website, which serves as a clearinghouse for resources on gender equality and empowerment of women. The fifth link will take users to a good history of International Women's Day, created by Joyce Stevens for the Australian Women's Intra Network. The final link leads to a well-honed site developed by the World Health Organization to highlight its own work on women's health (including special sections on work in Southeast Asia) and to bring together a number of useful fact sheets and related web-based resources.

  14. School Mathematics in Culture-Conflict Situations.

    ERIC Educational Resources Information Center

    Presmeg, Norma C.

    1988-01-01

    Suggests that mathematics curricula, which have traditionally been considered culture-free, have a role to play in fostering understanding among members of different cultures, after a period of cultural upheaval. Anthropological and educational sources are used to suggest points of relevance when a mathematical curriculum is designed for…

  15. TRADURRE IN ITALIANO IL TESTO SEGUENTE: The approximate number of days in a year is 365.2422, equal to 365 days 5 hours

    E-print Network

    Robbiano, Lorenzo

    revo- lution of the Sun. Every civilization has related it to the period of the moon's phases, for example the time between two new moons, which is approximately 29.5306 days, or 29 days 12 hours 44 times the period of the Moon. In the Julian calendar the ordinary years have 365 days, but every year

  16. Chaos and periodicity in solar wind speed: cycle 23

    NASA Astrophysics Data System (ADS)

    Sarkar, Tushnik; Ray, Rajdeep; Khondekar, Mofazzal H.; Ghosh, Koushik; Banerjee, Subrata

    2015-06-01

    The solar wind speed time series data from 1st January, 1997 to 28th October, 2003 has been pre-processed using simple exponential smoothing, discrete wavelet transform for denoising to investigate the underneath dynamics of it. Recurrence plot and recurrence quantification analysis has revealed that the time series is non-stationary one with deterministic chaotic behavior. The Hilbert-Huang Transform has been used in search of the underlying periods of the data series. Present investigation has revealed the periods of 21 days, 32.5 days, 43.6 days, 148.86 days, 180.7 days, 355.5 days, 403.2 days, 413.6 days, 490.72 days, 729.6 days, 1086.76 days, 1599.4 days and 1892.6 days.

  17. Biodegradation of Palm Kernel Cake by Cellulolytic and Hemicellulolytic Bacterial Cultures through Solid State Fermentation

    PubMed Central

    Alshelmani, Mohamed Idris; Loh, Teck Chwen; Foo, Hooi Ling; Lau, Wei Hong; Sazili, Awis Qurni

    2014-01-01

    Four cellulolytic and hemicellulolytic bacterial cultures were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). Two experiments were conducted; the objective of the first experiment was to determine the optimum time period required for solid state fermentation (SSF) of palm kernel cake (PKC), whereas the objective of the second experiment was to investigate the effect of combinations of these cellulolytic and hemicellulolytic bacteria on the nutritive quality of the PKC. In the first experiment, the SSF was lasted for 12 days with inoculum size of 10% (v/w) on different PKC to moisture ratios. In the second experiment, fifteen combinations were created among the four microbes with one untreated PKC as a control. The SSF lasted for 9 days, and the samples were autoclaved, dried, and analyzed for proximate analysis. Results showed that bacterial cultures produced high enzymes activities at the 4th day of SSF, whereas their abilities to produce enzymes tended to be decreased to reach zero at the 8th day of SSF. Findings in the second experiment showed that hemicellulose and cellulose was significantly (P < 0.05) decreased, whereas the amount of reducing sugars were significantly (P < 0.05) increased in the fermented PKC (FPKC) compared with untreated PKC. PMID:25019097

  18. Overcoming bottlenecks of enzymatic biofuel cell cathodes: crude fungal culture supernatant can help to extend lifetime and reduce cost.

    PubMed

    Sané, Sabine; Jolivalt, Claude; Mittler, Gerhard; Nielsen, Peter J; Rubenwolf, Stefanie; Zengerle, Roland; Kerzenmacher, Sven

    2013-07-01

    Enzymatic biofuel cells (BFCs) show great potential for the direct conversion of biochemically stored energy from renewable biomass resources into electricity. However, enzyme purification is time-consuming and expensive. Furthermore, the long-term use of enzymatic BFCs is hindered by enzyme degradation, which limits their lifetime to only a few weeks. We show, for the first time, that crude culture supernatant from enzyme-secreting microorganisms (Trametes versicolor) can be used without further treatment to supply the enzyme laccase to the cathode of a mediatorless BFC. Polarization curves show that there is no significant difference in the cathode performance when using crude supernatant that contains laccase compared to purified laccase in culture medium or buffer solution. Furthermore, we demonstrate that the oxygen reduction activity of this enzymatic cathode can be sustained over a period of at least 120 days by periodic resupply of crude culture supernatant. This is more than five times longer than control cathodes without the resupply of culture supernatant. During the operation period of 120 days, no progressive loss of potential is observed, which suggests that significantly longer lifetimes than shown in this work may be possible. Our results demonstrate the possibility to establish simple, cost efficient, and mediatorless enzymatic BFC cathodes that do not require expensive enzyme purification procedures. Furthermore, they show the feasibility of an enzymatic BFC with an extended lifetime, in which self-replicating microorganisms provide the electrode with catalytically active enzymes in a continuous or periodic manner. PMID:23801592

  19. Proceedings, Dean's Day 1999

    SciTech Connect

    Zanner, M.A.

    1999-03-01

    On January 14--15, 1999, Sandia National Laboratories sponsored Deans Day, a conference for the Deans of Engineering and other executive-level representatives from 29 invited universities. Through breakout sessions and a wrap-up discussion, university and Sandia participants identified activities to further develop their strategic relationships. The four primary activities are: (A) concentrate joint efforts on current and future research strengths and needs; (B) attract the best students (at all grade levels) to science and engineering; (C) promote awareness of the need for and work together to influence a national science and technology R and D policy; and (D) enable the universities and Sandia to be true allies, jointly pursuing research opportunities and funding from government agencies and industry.

  20. National Estuaries Day

    NSDL National Science Digital Library

    Get the most out of National Estuaries Day (October 5, 2002) by visiting this Web site from the National Oceanic and Atmospheric Administration (NOAA). Follow a link to Estuary Live!, which offers online interactive fieldtrips through a number of estuaries around the nation. Guided tours of eight estuaries will be webcast live October 3 and 4, supplemented by videos from a number of other estuaries. Internet participants "will have an opportunity to see the fascinating creatures that make estuaries their home and experience the diversity of estuarine ecosystems." Viewers may submit questions during the webcasts or videos, which will be answered by tour guides and educators from the featured estuaries. Click on About Estuaries for an introduction to estuarine ecosystems and for links to a number of Web sites that "provide general information, curriculums and helpful references on estuaries."

  1. Development of a serum-free defined system employing growth factors for preantral follicle culture.

    PubMed

    Park, Young Hyun; Gong, Seung Pyo; Kim, Hwa Young; Kim, Gil Ah; Choi, Jun Hee; Ahn, Ji Yeon; Lim, Jeong Mook

    2013-09-01

    This study was conducted to evaluate if mouse preantral follicles can yield developmentally competent oocytes following culture in serum-free, defined medium. Donor follicles were obtained from 14-day-old B6CBAF1 mice, and cultured in ?-MEM-Glutamax medium. The replacement of fetal bovine serum with knockout serum replacement (KSR) did not significantly reduce follicle growth or oocyte maturation in vitro, although it significantly reduced the development of oocytes after activation. Regardless of the replacement medium, follicle growth was not influenced by the addition of leukemia inhibitory factor (LIF). The addition of 100 ng/ml stem cell factor (SCF) to the KSR-supplemented serum-free medium significantly stimulated follicle development, which further improved blastocyst formation after oocyte activation. On Day 3 of culture, a significant increase in Bmp7 expression was detected in the SCF-containing medium compared with the serum-containing medium, whereas Gdf9 and Amh were increased in the serum-containing medium. A significant increase in estradiol production was detected under serum-free conditions, but minimal progesterone secretion was detected throughout the culture period. In conclusion, serum-free media can be used to optimize ovarian follicle cultures, and the addition of SCF is beneficial for deriving developmentally competent oocytes through follicle culture. PMID:23813589

  2. Myth or Truth: Independence Day.

    ERIC Educational Resources Information Center

    Gardner, Traci

    Most Americans think of the Fourth of July as Independence Day, but is it really the day the U.S. declared and celebrated independence? By exploring myths and truths surrounding Independence Day, this lesson asks students to think critically about commonly believed stories regarding the beginning of the Revolutionary War and the Independence Day…

  3. Family Day Care Training Curriculum.

    ERIC Educational Resources Information Center

    Nakatsu, Gail

    California's Family Day Care Training Program was designed to recruit and train in 7 weeks, Lao, Vietnamese, and Chinese refugees to establish their own state-licensed, family day care homes. Topics in the program's curriculum include an introduction to family day care, state licenses for family day care, state licensing requirements for family…

  4. Keys to Building Successful Training Programs for Hispanic Family Day Care Providers.

    ERIC Educational Resources Information Center

    Warrix, Marisa B.; Bocanegra, Margarita

    1998-01-01

    Critical factors for success in targeted training programs for Hispanic family day care providers include understanding the importance of Hispanic culture, values, and attitudes; becoming familiar with personalism and familism; using day care as an employment strategy for Hispanic women; and developing culturally relevant nutrition lessons. (SK)

  5. Day-to-day migrating and nonmigrating tidal variability due to the six-day planetary wave

    NASA Astrophysics Data System (ADS)

    Pedatella, N. M.; Liu, H.-L.; Hagan, M. E.

    2012-06-01

    To investigate day-to-day variability in the mesosphere and lower thermosphere (MLT), an idealized simulation of a six-day westward propagating zonal wave number-1 planetary wave is performed using the National Center for Atmospheric Research (NCAR) Thermosphere-Ionosphere-Mesosphere-Electrodynamics General Circulation Model (TIME-GCM). The six-day planetary wave introduces a six-day periodicity in the zonal mean atmosphere, migrating and nonmigrating tides, as well as in secondary waves that are produced by nonlinear planetary wave-tide interactions. We have further used the linear Global Scale Wave Model (GSWM) to isolate the effect of how the day-to-day changes in zonal mean zonal winds may influence tides in the MLT. The most significant changes are observed in the migrating diurnal tide (DW1), eastward propagating nonmigrating tides with zonal wave numbers-2 and -3 (DE2 and DE3), and a 20 hr eastward propagating wave with zonal wave number-2 (20E2). Because we have included the lower atmospheric source of nonmigrating tides, DE2 and DE3 are present with relatively large amplitudes in the MLT, even in the absence of planetary wave forcing. The 20E2 wave is produced by the nonlinear interaction between the DE3 and the six-day planetary wave, and its large amplitude indicates the importance of including the realistic spectra of nonmigrating tides in numerical simulations of planetary waves. The GSWM simulations reveal that the DW1 is not significantly influenced by the changes in the zonal mean winds. We thus conclude that the DW1 changes are driven by a combination of changes due to nonlinear interaction with the six-day planetary wave as well as changes due to zonal asymmetries that result from the six-day planetary wave. The six-day planetary wave induced changes in zonal mean zonal winds lead to a general reduction in the amplitude of DE2 and DE3, and introduce a slight periodic behavior in these tides. The effect of changing zonal mean zonal winds appears to be the primary driver of the changes in the DE2. However, for DE3, although the changes that can be attributed to zonal mean zonal wind variability are not insignificant, the primary driver of the DE3 perturbations appears to be the nonlinear interaction with the six-day planetary wave. Last, we demonstrate that the day-to-day changes in the DE3 introduce similar day-to-day changes in the daytime wave number-4 longitude structure in the low-latitude ionosphere. These results indicate that short-term variability in the low-latitude ionosphere is likely to be driven by similar short-term variability in nonmigrating tides in the MLT.

  6. PSY 344. Psychology and Culture SDSU Fall 2013 Schedule # 22551

    E-print Network

    Gallo, Linda C.

    PSY 344. Psychology and Culture SDSU ­ Fall 2013 ­ Schedule # 22551 Class Meetings Class Days of the course is to introduce you to the field of cultural psychology. This course will explore how culture influences human thoughts, feelings, and behaviors. The relations between culture and psychology are both

  7. Nerve Growth Factor Receptors on Cultured Rat Schwann Cells

    Microsoft Academic Search

    Peter S. DiStefano; Eugene M. Johnson

    1988-01-01

    Neonatal rat Schwann ceils were grown in tissue culture and assayed for NGF receptors with time in culture. NGF receptor levels on freshly prepared Schwann cells (day 0) were low but increased dramatically during the first week in culture. Characterization of 1a51-NGF binding to resuspended cells grown for 4 d in culture revealed that binding was not sat- urable at

  8. Valentine's Day and Chocolate

    NSDL National Science Digital Library

    Grinnell, Max

    2003-01-01

    While the exact origins of Valentine's Day are often disputed, the link between chocolate and the modern incarnation of this particular holiday are undeniable. Recent data released in a report by the business information group Datamonitor revealed that the global consumption of chocolate totaled approximately $42.2 billion worth of chocolate-based consumable goods. It will not come as a surprise to anyone who has visited the British Isles to learn that out of the $42.2 billion, Britons spent $19.2 billion, and made almost one in three chocolate purchases, trumping all of the world's nations. The report from Datamonitor also noted that tastes in chocolate vary widely across Europe, noting that, "The French prefer simplicity and purity of taste, without additional flavors and with little sugar, while Italian tastes are geared toward the more indulgent and sophisticated end of the market." Additionally, the report also indicates that the United States continues to dominate the global chocolate confectionary market, with Americans spending $2.4 billion on boxed chocolates alone in 2001.The first link is to the recent report on global chocolate sales and consumption released by the Datamonitor group. The second link leads to a well-thought site from Exploratorium Magazine, which features interactive essays on chocolate production, the potential health benefits of chocolate, and a visit to a "chocolate party" in the heart of the Amazon. The third link will take visitors to a site that deals with the history of chocolate, beginning with the first use of chocolate (as a drink) in 750 AD. The fourth link takes visitors to a site featuring inventive and fun ways to use chocolate in recipes, such as those for chocolate banana bread and chocolate peanut butter pie. The fifth link leads to a site from ABC News.com that explores various contested origins of Valentine's Day over the past fifteen hundred years. The final link takes visitors to a piece written by Polly Curtis of The Guardian, writing on the various scientific studies that investigate various romantic activities, such as which direction do people tend to kiss or how to find the perfect partner.

  9. Cultural Communications.

    ERIC Educational Resources Information Center

    Armas, Jose

    It is too often taken for granted that the communication process with culturally different children takes place as readily as it might with children from Anglo cultures. Most teachers receive training in verbal and formal communication skills; children come to school with nonverbal and informal communication skills. This initially can create…

  10. Culture Contents.

    ERIC Educational Resources Information Center

    Phillips, Kevin

    2001-01-01

    Successful relations between the United States and United Kingdom may require more attention to their internal ethnic and cultural diversity. This political-linguistic culture is subdivided into two contradictory yet complementary streams. The political approach for those favoring an English-speaking union may be greater ecumenism within the…

  11. 17 CFR 287.2 - Periodic reports.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...EXCHANGE COMMISSION (CONTINUED) Schedule A to Part 285 GENERAL RULES AND REGULATIONS PURSUANT TO SECTION 11(a) OF THE ASIAN DEVELOPMENT BANK ACT § 287.2 Periodic reports. (a) Within 60 days after the end of each of its fiscal...

  12. 17 CFR 287.2 - Periodic reports.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...EXCHANGE COMMISSION (CONTINUED) Schedule A to Part 285 GENERAL RULES AND REGULATIONS PURSUANT TO SECTION 11(a) OF THE ASIAN DEVELOPMENT BANK ACT § 287.2 Periodic reports. (a) Within 60 days after the end of each of its fiscal...

  13. 17 CFR 287.2 - Periodic reports.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...EXCHANGE COMMISSION (CONTINUED) Schedule A to Part 285 GENERAL RULES AND REGULATIONS PURSUANT TO SECTION 11(a) OF THE ASIAN DEVELOPMENT BANK ACT § 287.2 Periodic reports. (a) Within 60 days after the end of each of its fiscal...

  14. 17 CFR 287.2 - Periodic reports.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...EXCHANGE COMMISSION (CONTINUED) Schedule A to Part 285 GENERAL RULES AND REGULATIONS PURSUANT TO SECTION 11(a) OF THE ASIAN DEVELOPMENT BANK ACT § 287.2 Periodic reports. (a) Within 60 days after the end of each of its fiscal...

  15. 17 CFR 287.2 - Periodic reports.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...EXCHANGE COMMISSION (CONTINUED) Schedule A to Part 285 GENERAL RULES AND REGULATIONS PURSUANT TO SECTION 11(a) OF THE ASIAN DEVELOPMENT BANK ACT § 287.2 Periodic reports. (a) Within 60 days after the end of each of its fiscal...

  16. The Incubation Periods of Dengue Viruses

    PubMed Central

    Chan, Miranda; Johansson, Michael A.

    2012-01-01

    Dengue viruses are major contributors to illness and death globally. Here we analyze the extrinsic and intrinsic incubation periods (EIP and IIP), in the mosquito and human, respectively. We identified 146 EIP observations from 8 studies and 204 IIP observations from 35 studies. These data were fitted with censored Bayesian time-to-event models. The best-fitting temperature-dependent EIP model estimated that 95% of EIPs are between 5 and 33 days at 25°C, and 2 and 15 days at 30°C, with means of 15 and 6.5 days, respectively. The mean IIP estimate was 5.9 days, with 95% expected between days 3 and 10. Differences between serotypes were not identified for either incubation period. These incubation period models should be useful in clinical diagnosis, outbreak investigation, prevention and control efforts, and mathematical modeling of dengue virus transmission. PMID:23226436

  17. Nutrition on match day.

    PubMed

    Williams, Clyde; Serratosa, Luis

    2006-07-01

    What players should eat on match day is a frequently asked question in sports nutrition. The recommendation from the available evidence is that players should eat a high-carbohydrate meal about 3 h before the match. This may be breakfast when the matches are played around midday, lunch for late afternoon matches, and an early dinner when matches are played late in the evening. The combination of a high-carbohydrate pre-match meal and a sports drink, ingested during the match, results in a greater exercise capacity than a high-carbohydrate meal alone. There is evidence to suggest that there are benefits to a pre-match meal that is composed of low-glycaemic index (GI) carbohydrate foods rather than high-GI foods. A low-GI pre-match meal results in feelings of satiety for longer and produces a more stable blood glucose concentration than after a high-GI meal. There are also some reports of improved endurance capacity after low-GI carbohydrate pre-exercise meals. The physical demands of soccer training and match-play draw heavily on players' carbohydrate stores and so the benefits of good nutritional practices for performance and health should be an essential part of the education of players, coaches, and in particular the parents of young players. PMID:16766498

  18. Object of the Day

    NSDL National Science Digital Library

    The Smithsonian Cooper-Hewitt Design Museum is closed for renovation until 2014. Until then, visit this website to "Discover a different object from the Museum's collection every day of the week!" Items range from a 1938 catalog of linoleum and cork flooring, The Armstrong Pattern Book, to scratch and sniff wallpaper designed by Michael Angelo and produced by Flavor Paper in 2007. Information about each object is presented blog-post style, with a short essay, images, and tags that link to related information. For example, the entry for Saturday June 22, 2013, titled Two Rooks, is about a a ceramic tile made at Rookwood Pottery in Cincinnati, Ohio between 1903 and 1913. There a short essay about the tile by Cynthia E. Smith, one of the curators, and clicking the author's name retrieves all of her posts. Other tags include the subject area Arts and Crafts, the style of the piece, and Cincinnati, where it was made. There's also a link to click through to the full collection record for the tile, with the object's location, acquisition information, and color palette.

  19. Development of Nitrogen Assimilation Enzymes during Photoautotrophic Growth of Chenopodium rubrum Suspension Cultures.

    PubMed

    Campbell, W H; Ziegler, P; Beck, E

    1984-04-01

    Chenopodium rubrum cells were grown in suspension as a photoautotrophic culture with a 16 hour day. Cell growth had three phases: a 3-day lag, a 3-week logarithmic phase, and a 10-day stationary phase. Chlorophyll content increased steadily during log phase and reached a level of 0.5 to 0.6 mg Chl g(-1) fresh weight. Soluble protein of the cells increased more rapidly from day 4 to day 12 than during midlog phase. Initially, ammonium was taken up in preference to nitrate. However, during the second two weeks of growth, ammonium and nitrate were taken up simultaneously; this period of growth was the time of highest rates of N uptake by the cultured cells. Glutamine synthetase had a high specific activity (17 mumol.hour(-1) mg(-1) protein) in day 1 cells, and this level was sustained until midlog phase when it increased by 20%. Methyl viologen-dependent glutamate synthase specific activity increased rapidly in lag phase cells (day 4 = 10 mumol.hour(-1) mg(-1) protein), but decreased by day 9 to about 50% of the peak and remained constant. NADH:nitrate reductase specific activity increased rapidly in lag phase cells and reached a plateau that lasted from day 4 to 14 (1 mumol.hour(-1) mg(-1) protein). Methyl viologen-dependent nitrite reductase specific activity was high when assayed on day 5 and increased to a maximum on day 15 to 16 (12 mumol.hour(-1) mg(-1) protein). NADPH- and NADH-dependent glutamate dehydrogenase specific activities remained rather constant throughout the growth cycle. The cells appeared to have developed photosynthetic competence and to have leaf-like activities of nitrogen assimilation enzymes. PMID:16663539

  20. Development of Nitrogen Assimilation Enzymes during Photoautotrophic Growth of Chenopodium rubrum Suspension Cultures 1

    PubMed Central

    Campbell, Wilbur H.; Ziegler, Paul; Beck, Erwin

    1984-01-01

    Chenopodium rubrum cells were grown in suspension as a photoautotrophic culture with a 16 hour day. Cell growth had three phases: a 3-day lag, a 3-week logarithmic phase, and a 10-day stationary phase. Chlorophyll content increased steadily during log phase and reached a level of 0.5 to 0.6 mg Chl g?1 fresh weight. Soluble protein of the cells increased more rapidly from day 4 to day 12 than during midlog phase. Initially, ammonium was taken up in preference to nitrate. However, during the second two weeks of growth, ammonium and nitrate were taken up simultaneously; this period of growth was the time of highest rates of N uptake by the cultured cells. Glutamine synthetase had a high specific activity (17 ?mol·hour?1 mg?1 protein) in day 1 cells, and this level was sustained until midlog phase when it increased by 20%. Methyl viologen-dependent glutamate synthase specific activity increased rapidly in lag phase cells (day 4 = 10 ?mol·hour?1 mg?1 protein), but decreased by day 9 to about 50% of the peak and remained constant. NADH:nitrate reductase specific activity increased rapidly in lag phase cells and reached a plateau that lasted from day 4 to 14 (1 ?mol·hour?1 mg?1 protein). Methyl viologen-dependent nitrite reductase specific activity was high when assayed on day 5 and increased to a maximum on day 15 to 16 (12 ?mol·hour?1 mg?1 protein). NADPH- and NADH-dependent glutamate dehydrogenase specific activities remained rather constant throughout the growth cycle. The cells appeared to have developed photosynthetic competence and to have leaf-like activities of nitrogen assimilation enzymes. PMID:16663539

  1. Biosynthesis of titin in cultured skeletal muscle cells

    SciTech Connect

    Isaacs, W.B.; Kim, I.S.; Struve, A.; Fulton, A.B. (Univ. of Iowa, Iowa City (USA))

    1989-11-01

    Although significant progress has been made regarding the structure and function of titin, little data exist on the biosynthesis of this large protein in developing muscle. Using pulse-labeling with ({sup 35}S)methionine and immunoprecipitation with an anti-titin mAb, we have examined the biosynthesis of titin in synchronized cultures of skeletal muscle cells derived from day 12 chicken embryos. We find that: (a) titin synthesis increases greater than 4-fold during the first week in culture and during this same time period, synthesis of muscle-specific myosin heavy chain increases greater than 12-fold; (b) newly synthesized titin has a t1/2 of approximately 70 h; (c) titin is resistant to extraction with Triton X-100 both during and immediately after its synthesis. These observations suggest that newly synthesized titin molecules are stable proteins that rapidly associate with the cytoskeleton of developing myotubes.

  2. Heavy Metal Humor: Reconsidering Carnival in Heavy Metal Culture

    E-print Network

    Powell, Gary Botts

    2013-06-05

    ’ work to modern-day metal culture, Powell draw parallels to between Bakhtin’s carnivalesque theory and metal culture with two different, exemplary “humorous” metal performances, GWAR and Anal Cunt. Powell chooses “humorous” metal groups because...

  3. A slope variation in the period-luminosity relation for short period SMC Cepheids

    Microsoft Academic Search

    F. Bauer; C. Afonso; J. N. Albert; C. Alard; J. Andersen; R. Ansari; É. Aubourg; P. Bareyre; J. P. Beaulieu; A. Bouquet; S. Char; X. Charlot; F. Couchot; C. Coutures; F. Derue; R. Ferlet; C. Gaucherel; J. F. Glicenstein; B. Goldman; A. Gould; D. Graff; M. Gros; J. Haissinski; J. C. Hamilton; D. Hardin; J. de Kat; A. Kim; T. Lasserre; É. Lesquoy; C. Loup; C. Magneville; B. Mansoux; J. B. Marquette; É. Maurice; A. Milsztajn; M. Moniez; N. Palanque-Delabrouille; O. Perdereau; L. Prévot; C. Renault; N. Regnault; J. Rich; M. Spiro; A. Vidal-Madjar; L. Vigroux; S. Zylberajch

    1999-01-01

    We present the Period-Luminosity relations from 290 Cepheids towards the LMC and 590 Cepheids towards the SMC. The two data sets were obtained using the two wide field CCD cameras of the EROS 2 microlensing survey. We observe a significant slope change of the period-luminosity relation for the SMC fundamental mode Cepheids with periods shorter than 2 days. Many possible

  4. Three-dimensional culture of differentiated endometrial stromal cells to oligodendrocyte progenitor cells (OPCs) in fibrin hydrogel.

    PubMed

    Asmani, Mohammad Nabi; Ai, Jafar; Amoabediny, Ghasem; Noroozi, Abbas; Azami, Mahmoud; Ebrahimi-Barough, Somayeh; Navaei-Nigjeh, Mona; Ai, Armin; Jafarabadi, Mina

    2013-12-01

    Neural tissue engineering is one of the most promising strategies for treatment of nerve tissue injuries. Three-dimensional (3D) environment mimics in vivo conditions for cells. 3D distribution and growth of the cells within the scaffold are both important for neural tissue engineering. In this study, endometrial stromal cell-derived oligodendrocyte progenitor cells (EnSC-derived OPCs) were cultured in fibrin gel and cell differentiation and viability were evaluated after 8 days of post-culture. The structural and mechanical characteristics of fibrin gel-like scaffold were examined with rheological analysis. EnSCs were isolated from donor tissue and were induced to OPCs with growth factors (FGF2/EGF/PDGF-AA) for 12 days, then were cultured in fibrin gel with Triiodothyronine (T3) medium for another 8 days. The viability of cells was analyzed using MTT assay for a period of 8 days culturing in a fibrin matrix. Structure of fibrin matrix and cell morphology was analyzed with SEM. TEM, immunostaining and quantitative RT-PCR was performed for OPCs markers after cell culturing in fibrin matrix. Cell viability is enhanced in fibrin matrix after 8 days. SEM and TEM show that cells are in good integration with nano-fibers. Moreover, immunohistochemistry and quantitative RT-PCR of OPCs differentiation markers showed that Olig2, Sox10, PDGFRa, CNP, and A2B5 are expressed after 8 days culturing within fibrin matrix. Fibrin can provide a suitable 3-D scaffold for EnSCs differentiated cells for the regeneration of CNS. PMID:24038753

  5. Induction and analysis of the alkaloid mitragynine content of a Mitragyna speciosa suspension culture system upon elicitation and precursor feeding.

    PubMed

    Mohamad Zuldin, Nor Nahazima; Said, Ikram Md; Mohd Noor, Normah; Zainal, Zamri; Jin Kiat, Chew; Ismail, Ismanizan

    2013-01-01

    This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in a Mitragyna speciosa suspension culture. The best callus induction was achieved from petiole explants cultured on WPM that was supplemented with 4?mg?L?¹ 2,4-D (70.83%). Calli were transferred to liquid media and agitated on rotary shakers to establish Mitragyna speciosa cell suspension cultures. The optimum settled cell volume was achieved in the presence of WPM that contained 3?mg?L?¹ 2,4-D and 3% sucrose (9.47 ± 0.4667?mL). The treatment of cultures with different concentrations of yeast extract and salicylic acid for different inoculation periods revealed that the highest mitragynine content as determined by HPLC was achieved from the culture treated with 250?mg?L?¹ yeast extract (9.275 ± 0.082?mg?L?¹) that was harvested on day 6 of culturing; salicylic acid showed low mitragynine content in all concentrations used. Tryptophan and loganin were used as exogenous precursors; the highest level of mitragynine production was achieved in cultures treated with 3? ?M tryptophan and harvested at 6 days (13.226 ± 1.98?mg?L?¹). PMID:24065873

  6. Induction and Analysis of the Alkaloid Mitragynine Content of a Mitragyna speciosa Suspension Culture System upon Elicitation and Precursor Feeding

    PubMed Central

    Mohamad Zuldin, Nor Nahazima; Said, Ikram Md.; Mohd Noor, Normah; Zainal, Zamri; Jin Kiat, Chew; Ismail, Ismanizan

    2013-01-01

    This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in a Mitragyna speciosa suspension culture. The best callus induction was achieved from petiole explants cultured on WPM that was supplemented with 4?mg?L?1 2, 4-D (70.83%). Calli were transferred to liquid media and agitated on rotary shakers to establish Mitragyna speciosa cell suspension cultures. The optimum settled cell volume was achieved in the presence of WPM that contained 3?mg?L?1 2,4-D and 3% sucrose (9.47 ± 0.4667?mL). The treatment of cultures with different concentrations of yeast extract and salicylic acid for different inoculation periods revealed that the highest mitragynine content as determined by HPLC was achieved from the culture treated with 250?mg?L?1 yeast extract (9.275 ± 0.082?mg?L?1) that was harvested on day 6 of culturing; salicylic acid showed low mitragynine content in all concentrations used. Tryptophan and loganin were used as exogenous precursors; the highest level of mitragynine production was achieved in cultures treated with 3??M tryptophan and harvested at 6 days (13.226 ± 1.98?mg?L?1). PMID:24065873

  7. Probability analysis of dry-day sequences in Texas 

    E-print Network

    Milne, Robert James

    1971-01-01

    , Texas 60 27 Estimated probabilities of 7-dry-day sequences for San Angelo, Texas. 61 28 Subjective curves of probabilities of dry days derived from the monthly mean number of rain days. 29 Probability of a dry day compared to the prob- ability of a 7... of probabilities of the occurrence or non-occurrence of rain- fall have become of increasing importance. Such studies of the prob- ability of precipitation are embodied in the realm of climatology. In forecasting precipitation for periods of more than a few days...

  8. Modeling the contribution of individual proteins to mixed skeletal muscle protein synthetic rates over increasing periods of label incorporation.

    PubMed

    Miller, Benjamin F; Wolff, Christopher A; Peelor, Fredrick F; Shipman, Patrick D; Hamilton, Karyn L

    2015-03-15

    Advances in stable isotope approaches, primarily the use of deuterium oxide ((2)H2O), allow for long-term measurements of protein synthesis, as well as the contribution of individual proteins to tissue measured protein synthesis rates. Here, we determined the influence of individual protein synthetic rates, individual protein content, and time of isotopic labeling on the measured synthesis rate of skeletal muscle proteins. To this end, we developed a mathematical model, applied the model to an established data set collected in vivo, and, to experimentally test the impact of different isotopic labeling periods, used (2)H2O to measure protein synthesis in cultured myotubes over periods of 2, 4, and 7 days. We first demonstrated the influence of both relative protein content and individual protein synthesis rates on measured synthesis rates over time. When expanded to include 286 individual proteins, the model closely approximated protein synthetic rates measured in vivo. The model revealed a 29% difference in measured synthesis rates from the slowest period of measurement (20 min) to the longest period of measurement (6 wk). In support of these findings, culturing of C2C12 myotubes with isotopic labeling periods of 2, 4, or 7 days revealed up to a doubling of the measured synthesis rate in the shorter labeling period compared with the longer period of labeling. From our model, we conclude that a 4-wk period of labeling is ideal for considering all proteins in a mixed-tissue fraction, while minimizing the slowing effect of fully turned-over proteins. In addition, we advocate that careful consideration must be paid to the period of isotopic labeling when comparing mixed protein synthetic rates between studies. PMID:25593288

  9. IN HONOR OF VETERANS DAY 2011 VETERANS DAY ACTIVITIES & DISCOUNTS

    E-print Network

    Ravikumar, B.

    IN HONOR OF VETERANS DAY 2011 VETERANS DAY ACTIVITIES & DISCOUNTS 1 8 0 1 E A S T C O T A T I A V E With Veterans Day fast approaching on November 11th , Sonoma State University would like to thank all of our student veterans who have served honorably in the military. SSU appreciates your service to our country

  10. Making Connections through Cultural Memory, Cultural Performance, and Cultural Translation.

    ERIC Educational Resources Information Center

    Irwin, Rita L.; Rogers, Tony; Wan, Yuh-Yao

    1999-01-01

    Addresses the need for making connections between cultures, especially among Aboriginal and dominant cultures. Focuses on these themes: cultural memory, cultural performance, and cultural translation. Highlights three Aboriginal cultures on three continents (South Australia, Canada, and Taiwan) to encourage art educators and students to engage in…

  11. The Impact of Day-Trading on Volatility and Liquidity

    Microsoft Academic Search

    Jay M. Chung; Hyuk Choe; Bong-Chan Kho

    2009-01-01

    We examine day-trading activities for 540 stocks traded on the Korea Stock Exchange using transactions data for the period from 1999 to 2000. Our cross-sectional analysis reveals that day-traders prefer lower-priced, more liquid, and more volatile stocks. By estimating various bivariate VAR models using minute-by-minute data, we find that greater day- trading activity leads to greater return volatility and that

  12. Clonal distribution of osteoprogenitor cells in cultured chick periostea: Functional relationship to bone formation

    SciTech Connect

    McCulloch, C.A.; Fair, C.A.; Tenenbaum, H.C.; Limeback, H.; Homareau, R. (Univ. of Toronto, Ontario (Canada))

    1990-08-01

    Folded explants of periosteum from embryonic chick calvaria form bone-like tissue when grown in the presence of ascorbic acid, organic phosphate, and dexamethasone. All osteoblast-like cells in these cultures arise de novo by differentiation of osteoprogenitor cells present in the periosteum. To study the spatial and functional relationships between bone formation and osteoprogenitor cells, cultures were continuously labeled with (3H)thymidine for periods of 1-5 days. Radioautographs of serial 2-microns plastic sections stained for alkaline phosphatase (AP) showed maximal labeling of 30% of fibroblastic (AP-negative) cells by 3 days while osteogenic cells (AP-positive) exhibited over 95% labeling by 5 days. No differential shifts in labeling indices, grain count histograms of fibroblastic and osteogenic cells or numbers of AP-positive cells were observed, indicating no significant recruitment of cells from the fibroblastic to the osteogenic compartment. Despite the continuous presence of (3H)thymidine, less than 35% of both osteoblasts and osteocytes were labeled at 5 days, indicating that only one-third of the osteoprogenitor cells had cycled prior to differentiation. Spatial clustering of (3H)thymidine-labeled cells was measured by computer-assisted morphometry and application of the Poisson distribution to assess contagion. Cluster size and number of labeled cells per cluster did not vary between 1-3 days, but the number of clusters increased 20-fold between Day 1 and Day 3. Three-dimensional reconstruction from serial sections showed that clusters formed long, tubular arrays of osteogenic cells up to eight cells in length and located within 2-3 cell layers from the bone surface. Selective killing of S-phase cells with two pulse labels of high specific activity (3H)thymidine at 1 and 2 days of culture completely blocked bone formation.

  13. National Adult Day Services Association

    MedlinePLUS

    THE NATIONAL VOICE FOR THE ADULT DAY SERVICE COMMUNITY NADSA advances the national development, recognition and use of adult day services. They provide a coordinated program of professional and compassionate supervised ...

  14. School Day Just Got Healthier

    MedlinePLUS

    ... 32 million children receive meals throughout the school day. These meals are based on nutrition standards from ... served at school. more fruits and vegetables every day Kids have fruits and vegetables at school every ...

  15. Last Days of Life (PDQ)

    MedlinePLUS

    ... Ask Your Doctor about Advanced Cancer Research Last Days of Life (PDQ®) Overview The end of life ... death. Knowing what to expect in the final days or hours helps comfort the family. Most people ...

  16. Cultural Diversity and the Performance of Multinational Firms

    Microsoft Academic Search

    Luis R. Gómez-mejia; Leslie E. Palich

    1997-01-01

    We test the hypothesis that culturally related international diversification will have a positive impact on firm performance and that the opposite will be true for culturally unrelated globalization. Cultural diversity for Fortune 500 firms was used to predict performance over a ten-year period (1985–1994), controlling for several organizational and industry characteristics. Regression tests using nine indicators of cultural diversity revealed

  17. A cultural, customizable and prefabricated housing grammar for Casablanca

    E-print Network

    Akkar, Ghita

    2011-01-01

    Proposing an innovative design grammar linking prefabrication, customization and cultural adaptability, this thesis addresses the present day housing deficit and lack of architectural identity in Casablanca, Morocco. The ...

  18. Effect of Clarias gariepinus cultured in contaminated water on rats.

    PubMed

    Adeyemi, Oyeyemi; Adeyemi, Olalekan; Osubor, Chris C

    2009-12-01

    In this study, African Catfish (Clarias gariepinus) were cultured in water contaminated with phthalate, benzene and cyclohexane (10 microg/mL), respectively, over a period of 65 days. They were, thereafter, used as protein source to formulate feed for albino rats (Wistar strain) for 28 days. The weights of the body and selected tissues of the rats were monitored and standard enzyme assays were conducted for some enzymes in the serum. Results showed that rats in cyclohexane group gained 58% whereas the control group gained 46% of body weight. Liver weight (absolute and relative) of test rats were found to be significantly lower than that of control (p < .05). Enzyme activity of serum of test rats was found to be significantly higher than that of control (p < .05). Experimental evidence suggests leakage of enzymes from the liver to the serum, thus the elevated serum enzyme activity and that the contaminants may damage the liver. PMID:19887379

  19. Blood culture

    MedlinePLUS

    Culture - blood ... A blood sample is needed. The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  20. Paramilitary Culture.

    ERIC Educational Resources Information Center

    Gibson, James William

    1989-01-01

    Identifies the movie, "Rambo," and "Soldier of Fortune" magazine as artifacts of "paramilitary culture." Contends that they are a social phenomenon which helps legitimate the United States government's rapid escalation of military forces. (MS)

  1. Stool Culture

    MedlinePLUS

    ... cultures when someone has had a previous pathogenic bacterial infection of the gastrointestinal tract and has either been ... there anything else I should know? Severe pathogenic bacterial infections of the gastrointestinal tract and those causing complications ...

  2. Individual differences in the day-to-day experience of chronic pain: a prospective daily study of rheumatoid arthritis patients.

    PubMed

    Affleck, G; Tennen, H; Urrows, S; Higgins, P

    1991-01-01

    Explored the distribution and temporal patterning of daily pain reported by 47 patients with rheumatoid arthritis (RA) for 75 consecutive days. Approximately half the pain series were significantly positively skewed, trended significantly across the recording period, or both. One fourth of the sample had relatively painful "outlier" days that clustered together. Most series displayed a significant autocorrelation in pain intensity across successive days even when the series were detrended. Patients with more active disease had pain that was more intense but more predictable from day to day and reported fewer painful outlying days and briefer episodes of atypically severe pain. Patients describing themselves as more depressed on the Center for Epidemiological Stress Depression Scale also reported more intense pain across the recording period, independent of their level of disease activity and disability. Implications for daily process studies of RA pain are discussed. PMID:1765037

  3. Perspectives on Infant Day Care.

    ERIC Educational Resources Information Center

    Elardo, Richard, E.; Pagan, Betty, Ed.

    These proceedings of the first annual SACUS workshop on infant day care contain the papers presented at the conference, plus an appendix--Developmental Objectives for Infants and Toddlers. The papers are: "Infant Day Care--Fads, Facts, and Fancies" by Bettye M. Caldwell; "Family Day Care""A Broad Perspective" by Malcolm S. Host; "Getting…

  4. Family Day Care Provider Handbook

    ERIC Educational Resources Information Center

    New York State Office of Children and Family Services, 2006

    2006-01-01

    Family day care providers are responsible for creating a high-quality program where children have opportunities to grow, learn and thrive. Part of providing high-quality child care includes complying with the family day care regulations from the New York State Office of Children and Family Services (OCFS). This Handbook will help day care…

  5. Fifth day fits in the newborn.

    PubMed

    North, K N; Storey, G N; Henderson-Smart, D J

    1989-10-01

    During the 1970s, there was an epidemic of neonatal convulsions occurring in apparently normal babies during the fourth and fifth days of life. This syndrome was noted in France as well as in Australia. A study was undertaken to review the King George V Hospital (KGV) experience with these 'fifth day fitters'. All cases of neonatal convulsions at KGV during the years 1972-85 were reviewed. A total of 94 cases of fifth day fits occurred during this period, accounting for 57% of all neonatal convulsions occurring during 1972-79. The fits occurred in healthy term infants after an uncomplicated pregnancy. They appeared on the fourth and fifth days of life. The seizures lasted an average of 24 h, were refractory to drug therapy and, despite extensive investigation, no cause was found. The infants were assessed as normal at the time of discharge from hospital. Follow-up of these infants was incomplete. However, from the data available, it cannot be assumed to be a benign entity. The 'fifth day fit' syndrome reached epidemic proportions at a number of maternity units during the 1970s. At KGV, no case has been observed since 1982. PMID:2590128

  6. Period Determination for 4527 Schoenberg

    NASA Astrophysics Data System (ADS)

    Burkhonov, O. A.; Ehgamberdiev, Sh. A.; Ergashev, K. E.

    2013-10-01

    The main-belt asteroid 4527 Schoenberg (1982 OK) has been observed between June 28 and July 1, 2012 at Maidanak astronomical observatory of the Ulugh Beg Astronomical Institute (UBAI), Uzbekistan Academy of Sciences. On the basis of data analysis it is found a synodic rotation period of 2.6928" b0.0384 hour (0.1122±0.0016 day) and lightcurve amplitude of 0.31±0.05 mag.

  7. Discrete choice models of traveler participation in differential time of day pricing programs

    E-print Network

    Burris, Mark W.

    congested periods of the day to less congested periods. Variable pricing generally involves charging more Tolls that vary based on time of day or congestion are gaining attention around the world as a potential contributing to peak period congestion relief. However, despite the widespread interest in the concept

  8. 42 CFR 408.8 - Grace period and termination date.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...HUMAN SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE General Provisions § 408.8 Grace period and...grace period ends with the last day of the third month after the billing month. (2) For payments required because the...

  9. 42 CFR 408.8 - Grace period and termination date.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...HUMAN SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE General Provisions § 408.8 Grace period and...grace period ends with the last day of the third month after the billing month. (2) For payments required because the...

  10. 42 CFR 408.8 - Grace period and termination date.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...HUMAN SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE General Provisions § 408.8 Grace period and...grace period ends with the last day of the third month after the billing month. (2) For payments required because the...

  11. 42 CFR 408.8 - Grace period and termination date.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...HUMAN SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE General Provisions § 408.8 Grace period and...grace period ends with the last day of the third month after the billing month. (2) For payments required because the...

  12. 42 CFR 408.8 - Grace period and termination date.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...HUMAN SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE General Provisions § 408.8 Grace period and...grace period ends with the last day of the third month after the billing month. (2) For payments required because the...

  13. 16 CFR 1502.2 - Computation of time periods.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...Provisions § 1502.2 Computation of time periods. Whenever a time period for taking action is specified...Federal holidays are included in computing time. However, if the last day for taking such action falls on a Saturday, Sunday, or...

  14. Development of tissue culture techniques and hardware to study mineralization under microgravity conditions

    NASA Astrophysics Data System (ADS)

    van Loon, J. J. W. A.; Veldhuijzen, J. P.; Windgassen, E. J.; Brouwer, T.; Wattel, K.; van Vilsteren, M.; Maas, P.

    1994-08-01

    To study the effects of weightlessness on mouse fetal long bone rudiment growth and mineralization we have developed a tissue culture system for the Biorack facility of Spacelab. The technique uses standard liquid tissue culture medium, supplemented with Na-?-glycerophosphate, confined in gas permeable polyethylene bags mounted inside ESA Biorack Type I experiment containers. The containers can be flushed with an air/5% CO2 gas mixture necessary for the physiological bicarbonate buffer used. Small amounts of fluid can be introduced at the beginning (e.g. radioactive labels for incorporation studies) or at the end of the experiment (fixatives). A certain form of mechanical stimulation (continuous compression) can be used to counteract the, possibly, adverse effect of ?-gravity. Using 16 day old metatarsals the in vitro calcification process under ?-gravity conditions can be studied for a 4 day period.

  15. Phenotypic characterization of prostate cancer LNCaP cells cultured within a bioengineered microenvironment.

    PubMed

    Sieh, Shirly; Taubenberger, Anna V; Rizzi, Simone C; Sadowski, Martin; Lehman, Melanie L; Rockstroh, Anja; An, Jiyuan; Clements, Judith A; Nelson, Colleen C; Hutmacher, Dietmar W

    2012-01-01

    Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in normal and cancer cells. Since two-dimensional (2D) cultures lack the essential characteristics of the native cellular microenvironment, three-dimensional (3D) cultures have been developed to better mimic the natural extracellular matrix. To date, 3D culture systems have relied mostly on collagen and Matrigel™ hydrogels, allowing only limited control over matrix stiffness, proteolytic degradability, and ligand density. In contrast, bioengineered hydrogels allow us to independently tune and systematically investigate the influence of these parameters on cell growth and differentiation. In this study, polyethylene glycol (PEG) hydrogels, functionalized with the Arginine-glycine-aspartic acid (RGD) motifs, common cell-binding motifs in extracellular matrix proteins, and matrix metalloproteinase (MMP) cleavage sites, were characterized regarding their stiffness, diffusive properties, and ability to support growth of androgen-dependent LNCaP prostate cancer cells. We found that the mechanical properties modulated the growth kinetics of LNCaP cells in the PEG hydrogel. At culture periods of 28 days, LNCaP cells underwent morphogenic changes, forming tumor-like structures in 3D culture, with hypoxic and apoptotic cores. We further compared protein and gene expression levels between 3D and 2D cultures upon stimulation with the synthetic androgen R1881. Interestingly, the kinetics of R1881 stimulated androgen receptor (AR) nuclear translocation differed between 2D and 3D cultures when observed by immunofluorescent staining. Furthermore, microarray studies revealed that changes in expression levels of androgen responsive genes upon R1881 treatment differed greatly between 2D and 3D cultures. Taken together, culturing LNCaP cells in the tunable PEG hydrogels reveals differences in the cellular responses to androgen stimulation between the 2D and 3D environments. Therefore, we suggest that the presented 3D culture system represents a powerful tool for high throughput prostate cancer drug testing that recapitulates tumor microenvironment. PMID:22957009

  16. Biomass production and nutrient removal potential of water hyacinth cultured in sewage effluent

    SciTech Connect

    Reddy, K.R.; Hueston, F.M.; McKinn, T.

    1985-05-01

    Growth and nutrient uptake of water hyacinth (Eichhornia crassipes (Mart Solms)) cultured in sewage effluent were measured over a period of one year in a prototype wastewater treatment system which has been in operation at Walt Disney World near Orlando, Florida. Annual productivity of water hyacinth cultured in primary sewage effluent (Channel II) was found to be in the range of 5 to 27 g dry wt/m/sup 2/ day (23.6 dry tons/acre yr). Average growth rate during the months of May through October 1982 for hyacinth cultured in Channel II (primary sewage effluent) and Channel I (treated primary sewage effluent leaving Channel II) was about 16 g dry wt/m/sup 2/ (27 dry tons/acre yr), compared to the growth rate of 13 g dry wt/m/sup 2/ (22 dry tons/acre yr) for hyacinths cultured in secondary sewage effluent. Plants cultured in secondary sewage effluent generally had longer roots than the plants cultured in primary sewage effluent. A significant relationship was observed between the growth rate of hyacinth and the solar radiation. N and P concentration of the plant tissue were higher in the hyacinths cultured during winter months compared to the plants grown in summer months. Average N and P concentration of the plants cultured im primary sewage effluent were found to be 3.7% N and 0.94% P, respectively, while the plants cultured in secondary sewage effluent had a total N and P content of 2.8% N and 0.79% P. Nutrient ratios of the major plant nurtrients were found to be approximately the same as the nutrient ratios in the sewage effluent. Annual N and P uptake rates of hyacinth cultured in sewage effluent were found to be in the range of 1176 to 1193 kg N/ha yr and 321 to 387 kg P/ha yr, respectively.

  17. 76 FR 44573 - Child and Adult Care Food Program: National Average Payment Rates, Day Care Home Food Service...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-26

    ...National Average Payment Rates, Day Care Home Food Service Payment Rates...Rates for Sponsoring Organizations of Day Care Homes for the Period July 1, 2011...Rates for Sponsoring Organizations of Day Care Homes per Home (Per month rates...

  18. Periodic Variability in the Large-Scale Southern Hemisphere

    E-print Network

    Periodic Variability in the Large-Scale Southern Hemisphere Atmospheric Circulation David W. J. Thompson* and Elizabeth A. Barnes Periodic behavior in the climate system has important implications circulation exhibits marked periodicity on time scales of approximately 20 to 30 days. The periodicity is tied

  19. Tissue-culture light sheet fluorescence microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions.

    PubMed

    Pampaloni, Francesco; Berge, Ulrich; Marmaras, Anastasios; Horvath, Peter; Kroschewski, Ruth; Stelzer, Ernst H K

    2014-10-01

    Fluorescence long-term imaging of cellular processes in three-dimensional cultures requires the control of media supply, temperature, and pH, as well as minimal photodamage. We describe a system based on a light sheet fluorescence microscope (LSFM), which is optimized for long-term, multi-position imaging of three-dimensional in-gel cell cultures. The system integrates a stable culture condition control system in the optical path of the light-sheet microscope. A further essential element is a biocompatible agarose container suitable for the LSFM, in which any cell type can be cultured in different gel matrices. The TC-LSFM allows studying any in vitro cultured cell type reacting to, dividing in, or migrating through a three-dimensional extracellular matrix (ECM) gel. For this reason we called it "tissue culture-LSFM" (TC-LSFM). The TC-LSFM system allows fast imaging at multiple locations within a millimeter-sized ECM gel. This increases the number of analyzed events and allows testing population effects. As an example, we show the maturation of a cyst of MDCK (canine kidney epithelial) cells over a period of three days. Moreover, we imaged, tracked, and analyzed MDCK cells during the first five days of cell aggregate formation and discovered a remarkable heterogeneity in cell cycle lengths and an interesting cell death pattern. Thus, TC-LSFM allows performing new long-term assays assessing cellular behavior in three-dimensional ECM-gel cultures. For example migration, invasion or differentiation in epithelial cell systems, stem cells, as well as cancer cells can be investigated. PMID:25183478

  20. Rat retina shows robust circadian expression of clock and clock output genes in explant culture

    PubMed Central

    Buonfiglio, Daniella C.; Malan, André; Sandu, Cristina; Jaeger, Catherine; Cipolla-Neto, José; Hicks, David

    2014-01-01

    Purpose Circadian rhythms are central to vision and retinal physiology. A circadian clock located within the retina controls various rhythmic processes including melatonin synthesis in photoreceptors. In the present study, we evaluated the rhythmic expression of clock genes and clock output genes in retinal explants maintained for several days in darkness. Methods Retinas were dissected from Wistar rats, either wild-type or from the Per1-luciferase transgenic line housed under a daily 12 h:12 h light-dark cycle (LD12/12), and put in culture at zeitgeber time (ZT) 12 on semipermeable membranes. Explants from wild-type rats were collected every 4 h over 3 days, and total RNA was extracted, quantified, and reverse transcribed. Gene expression was assessed with quantitative PCR, and the periodicity of the relative mRNA amounts was assessed with nonlinear least squares fitting to sine wave functions. Bioluminescence in explants from Per1-luciferase rats was monitored for several days under three different culture protocols. Results Rhythmic expression was found for all studied clock genes and for clock downstream targets such as c-fos and arylalkylamine N-acetyltransferase (Aanat) genes. Clock and output genes cycled with relatively similar periods and acrophases (peaks of expression during subjective night, except c-fos, which peaked around the end of the subjective day). Data for Per1 were confirmed with bioluminescence monitoring, which also permitted culture conditions to be optimized to study the retina clock. Conclusions Our work shows the free-running expression profile of multiple clock genes and potential clock targets in mammalian retinal explants. This research further strengthens the notion that the retina contains a self-sustained oscillator that can be functionally characterized in organotypic culture. PMID:24940028

  1. Ureaplasma infection of cell cultures.

    PubMed Central

    Kotani, H; McGarrity, G J

    1986-01-01

    Studies were performed to characterize the effects of ureaplasmas in HeLa, 3T6, and CV-1 cell cultures. The ureaplasmas studied were human Ureaplasma urealyticum T960 (serotype VIII), bovine U. diversum T95, simian strain T167-2, ovine strain 1202, canine strain D1M-C, and feline strains 382 and FT2-B. FT2-B was the only ureaplasma to grow in the cell free culture medium, Dulbecco modified Eagle-Earle medium containing 10% fetal bovine serum. The growth pattern of the ureaplasmas varied in the different cell cultures, but each strain grew in at least two of the cell cultures, suggesting a requirement for a product of the cell culture and for low concentrations of urea. When growth occurred, organisms grew to concentrations that approached, but did not equal, those observed in 10B broth. Most, but not all, ureaplasmas grew quickly, reaching peak titers 2 days after infection. Canine strain D1M-C did not grow in 3T6, but showed rapid growth in HeLa and CV-1 cells, killing both cultures, In some systems, e.g., U. urealyticum T960 and simian strain T167-2, the infection persisted, and ureaplasmas could be recovered from cell cultures four passages after infection, when studies were terminated. The cell culture ureaplasmas grew on T agar, but not on mycoplasma agar medium. Images PMID:3699891

  2. Hydrogen as an electron donor for dechlorination of tetrachloroethene by an anaerobic mixed culture.

    PubMed Central

    DiStefano, T D; Gossett, J M; Zinder, S H

    1992-01-01

    Hydrogen served as an electron donor in the reductive dechlorination of tetrachloroethene to vinyl chloride and ethene over periods of 14 to 40 days in anaerobic enrichment cultures; however, sustained dechlorination for more extended periods required the addition of filtered supernatant from a methanol-fed culture. This result suggests a nutritional dependency of hydrogen-utilizing dechlorinators on the metabolic products of other organisms in the more diverse, methanol-fed system. Vancomycin, an inhibitor of cell wall synthesis in eubacteria, was found to inhibit acetogenesis when added at 100 mg/liter to both methanol-fed and hydrogen-fed cultures. The effect of vancomycin on dechlorination was more complex. Methanol could not sustain dechlorination when vancomycin inhibited acetogenesis, while hydrogen could. These results are consistent with a model in which hydrogen is the electron donor directly used for dechlorination by organisms resistant to vancomycin and with the hypothesis that the role of acetogens in methanol-fed cultures is to metabolize a portion of the methanol to hydrogen. Methanol and other substrates shown to support dechlorination in pure and mixed cultures may merely serve as precursors for the formation of an intermediate hydrogen pool. This hypothesis suggests that, for bioremediation of high levels of tetrachloroethene, electron donors that cause the production of a large hydrogen pool should be selected or methods that directly use H2 should be devised. PMID:1482184

  3. EAST ASIAN LANGUAGESEAST ASIAN LANGUAGESEAST ASIAN LANGUAGES & CULTURES& CULTURES& CULTURES

    E-print Network

    Krylov, Anna I.

    EAST ASIAN LANGUAGESEAST ASIAN LANGUAGESEAST ASIAN LANGUAGES & CULTURES& CULTURES& CULTURES typically concentrate on one East Asian language and culture while also taking broader survey courses: This program allows exceptional students to complete both a BA and MA in East Asian Languages and Cultures

  4. Catechin production in cultured Polygonum hydropiper cells

    Microsoft Academic Search

    Kanji Ono; Mayumi Nakao; Masao Toyota; Yoshimi Terashi; Masashi Yamada; Tetsuya Kohno; Yoshinori Asakawa

    1998-01-01

    Callus and suspension-cultured cells were induced from hypocotyls of Polygonum hydropiper seedlings. Both the callus and suspension-cultured cells produced mainly (+)-catechin accompanied by (?)-epicatechin and (?)-epicatechin-3-O-gallate. The (+)-catechin production of suspension-cultured cells increased with cell growth and reached the maximal value (29.0mgg?1 dry wt) after 6days from the start of subculture. This is the highest value of (+)-catechin content among

  5. In the Schools: California Treat: Three Days in Five Ecosystems.

    ERIC Educational Resources Information Center

    Rigby, Jennifer A.

    1986-01-01

    Describes a 3-day program sponsored by the Orange County Marine Institute that provides biological, cultural, and historical learning experiences. Discusses the setting and activities of the five ecosystems explored by the students. The Chaparral to Ocean Science Camp includes chaparral, riparian, woodland, intertidal, and pelagic environments.…

  6. Play in the Day of Qiaoqiao: A Chinese Perspective.

    ERIC Educational Resources Information Center

    Cooney, Margaret H.; Sha, Jin

    1999-01-01

    Explores the role of play in early-childhood education in China. Describes a typical day in the life of one Chinese child enrolled in a preprimary program. Cites research findings on which daily activities were perceived as play by the child, her parents, grandparents, and teacher. Concludes with a discussion about the impact of culture on…

  7. Cultural Correspondence

    NSDL National Science Digital Library

    In the early 1970s, Paul Buhle and Dave Wagner began to think about creating a new and unique journal. They were both veterans of the journal Radical America, and they were both interested in popular culture. As August 1975 came around, they published the first issue of "Cultural Correspondence", a journal intended to serve as a critical review of popular culture. As the journal was published in Providence, it's not surprising to learn that the Brown University Library Center for Digital Initiatives (CDL) has created this most engaging archive of this publication. Visitors to the site can read the entire contents of each issue, browse around as they see fit, and also check out their virtual "Magazine Stand". First-time visitors may wish to start out by reading the Spring 1977 issue titled "Television".

  8. Rethinking the Day of Silence

    ERIC Educational Resources Information Center

    Murphy, Adriana

    2013-01-01

    Back in 2006, 7th and 8th graders at Green Acres, the K-8 independent school where the author taught in suburban Maryland, participated in the Day of Silence. The Day of Silence is a national event: Students across the country take a one-day pledge of silence to show that they want to make schools safe for all students, regardless of their sexual…

  9. Study of the Half-Day/Full-Day Kindergarten Model

    ERIC Educational Resources Information Center

    McInroy, Thomas R.

    2012-01-01

    This case study and problem analysis was an in-depth investigation of the half-day/full-day kindergarten model by utilizing interviews and focus groups to provide insight from parents, teachers, and other district personnel as to how the model has impacted the social, emotional, and academic development of the participating students. This study…

  10. Day Delegate Rates Full Day Delegate Rate 32

    E-print Network

    Lasenby, Joan

    Plate Soup of the Day Supplement of £3.50 *** Dessert rè e Brûlée ith Rasp erries Le n art cha h c late Vegeta le hilli ith Rice Soup of the Day--Supplement of £3.50 Dessert rè e Brûlée ith Rasp erries Le n

  11. Earth rotation and GNSS orbits from one-day and three-day arcs

    NASA Astrophysics Data System (ADS)

    Lutz, Simon; Schaer, Stefan; Dach, Rolf; Beutler, Gerhard; Steigenberger, Peter; Meindl, Michael; Jäggi, Adrian

    2014-05-01

    The Center for Orbit Determination in Europe (CODE) is one of the analysis centers of the International GNSS Service (IGS). It is estimating satellite orbits and consistent sets of Earth rotation parameters (ERPs) for the final, rapid, and ultra-rapid product lines of the IGS. The solutions are derived from a combined multi-system (GPS and GLONASS) analysis of the GNSS tracking data. Since September 2012 two series of final solutions are operationally generated and submitted to the IGS: the first is based on the observations from exactly one day (requirement of the IGS) and the second stacks the one-day normal equations of three consecutive days to get orbital arcs and piecewise linear Earth rotation parameters which are continuous at the boundaries of the middle day. The same two solution types were produced for the second reprocessing campaign of the IGS (covering the interval from 1994 to the end of 2013; GLONASS starts in 2002). The estimation of the polar motion rates reveals serious deficiencies in the case of the one-day solutions (probably due to interferences with the sub-daily ERPs). Suspicious signatures in the time series of the estimated parameters not visible in the three-day solutions are systematically disturbing the results of the one-day solutions. Artifacts with periods typical for the GLONASS constellation are clearly visible in the one-day solutions, but to a much lesser extent in the three-day solutions. This becomes even more evident in an alternative series generated as consistent (even regarding the station selection) GPS- and GLONASS-only products over four years (2007-2011).

  12. Sun-Earth Day, 2001

    NASA Technical Reports Server (NTRS)

    Adams, Mitzi L.; Mortfield, P.; Hathaway, D. H.; Whitaker, Ann F. (Technical Monitor)

    2001-01-01

    To promote awareness of the Sun-Earth connection, NASA's Marshall Space Flight Center, in collaboration with the Stanford SOLAR Center, sponsored a one-day Sun-Earth Day event on April 27, 2001. Although "celebrated" on only one day, teachers and students from across the nation, prepared for over a month in advance. Workshops were held in March to train teachers. Students performed experiments, results of which were shared through video clips and an internet web cast. Our poster includes highlights from student experiments (grades 2 - 12), lessons learned from the teacher workshops and the event itself, and plans for Sun-Earth Day 2002.

  13. "Every Day He Has a Dream to Tell": Classroom Literacy Curriculum in a Full-Day Kindergarten

    ERIC Educational Resources Information Center

    Heydon, Rachel; Moffatt, Lyndsay; Iannacci, Luigi

    2015-01-01

    Within an era of change to early childhood education and care, this case study of kindergarten classroom literacy curricula sought to understand the production and effects of the curriculum within one urban, Canadian full-day kindergarten that included culturally and linguistically diverse children. Central was a concern for the place of…

  14. PERIOD ERROR ESTIMATION FOR THE KEPLER ECLIPSING BINARY CATALOG

    SciTech Connect

    Mighell, Kenneth J. [National Optical Astronomy Observatory, 950 North Cherry Avenue, Tucson, AZ 85719 (United States); Plavchan, Peter [NASA Exoplanet Science Institute, California Institute of Technology, Pasadena, CA 91125 (United States)

    2013-06-15

    The Kepler Eclipsing Binary Catalog (KEBC) describes 2165 eclipsing binaries identified in the 115 deg{sup 2} Kepler Field based on observations from Kepler quarters Q0, Q1, and Q2. The periods in the KEBC are given in units of days out to six decimal places but no period errors are provided. We present the PEC (Period Error Calculator) algorithm, which can be used to estimate the period errors of strictly periodic variables observed by the Kepler Mission. The PEC algorithm is based on propagation of error theory and assumes that observation of every light curve peak/minimum in a long time-series observation can be unambiguously identified. The PEC algorithm can be efficiently programmed using just a few lines of C computer language code. The PEC algorithm was used to develop a simple model that provides period error estimates for eclipsing binaries in the KEBC with periods less than 62.5 days: log {sigma}{sub P} Almost-Equal-To - 5.8908 + 1.4425(1 + log P), where P is the period of an eclipsing binary in the KEBC in units of days. KEBC systems with periods {>=}62.5 days have KEBC period errors of {approx}0.0144 days. Periods and period errors of seven eclipsing binary systems in the KEBC were measured using the NASA Exoplanet Archive Periodogram Service and compared to period errors estimated using the PEC algorithm.

  15. Period Error Estimation for the Kepler Eclipsing Binary Catalog

    NASA Astrophysics Data System (ADS)

    Mighell, Kenneth J.; Plavchan, Peter

    2013-06-01

    The Kepler Eclipsing Binary Catalog (KEBC) describes 2165 eclipsing binaries identified in the 115 deg2 Kepler Field based on observations from Kepler quarters Q0, Q1, and Q2. The periods in the KEBC are given in units of days out to six decimal places but no period errors are provided. We present the PEC (Period Error Calculator) algorithm, which can be used to estimate the period errors of strictly periodic variables observed by the Kepler Mission. The PEC algorithm is based on propagation of error theory and assumes that observation of every light curve peak/minimum in a long time-series observation can be unambiguously identified. The PEC algorithm can be efficiently programmed using just a few lines of C computer language code. The PEC algorithm was used to develop a simple model that provides period error estimates for eclipsing binaries in the KEBC with periods less than 62.5 days: log ? P ? - 5.8908 + 1.4425(1 + log P), where P is the period of an eclipsing binary in the KEBC in units of days. KEBC systems with periods >=62.5 days have KEBC period errors of ~0.0144 days. Periods and period errors of seven eclipsing binary systems in the KEBC were measured using the NASA Exoplanet Archive Periodogram Service and compared to period errors estimated using the PEC algorithm.

  16. The Somali Bantu: Their History and Culture. Culture Profile.

    ERIC Educational Resources Information Center

    Lehman, Dan Van; Eno, Omar

    This booklet is a basic introduction to the people, history, and cultures of the Somali Bantu. It is designed primarily for service providers and others assisting Somali Bantu refugees in their new communities in the United States. It focuses on: "Introduction"; "Land"; "People" (place in society and social structures); "History" (colonial period

  17. The potential use of constructed wetlands in a recirculating aquaculture system for shrimp culture.

    PubMed

    Lin, Ying-Feng; Jing, Shuh-Ren; Lee, Der-Yuan

    2003-01-01

    A pilot-scale constructed wetland unit, consisting of free water surface (FWS) and subsurface flow (SF) constructed wetlands arranged in series, was integrated into an outdoor recirculating aquaculture system (RAS) for culturing Pacific white shrimp (Litopenaeus vannamei). This study evaluated the performance of the wetland unit in treating the recirculating wastewater and examined the effect of improvement in water quality of the culture tank on the growth and survival of shrimp postlarvae. During an 80-day culture period, the wetland unit operated at a mean hydraulic loading rate of 0.3 m/day and effectively reduced the influent concentrations of 5-day biochemical oxygen demand (BOD5, 24%), suspended solids (SS, 71%), chlorophyll a (chl-a, 88%), total ammonium (TAN, 57%), nitrite nitrogen (NO2-N, 90%) and nitrate nitrogen (NO3-N, 68%). Phosphate (PO4-P) reduction was the least efficient (5.4%). The concentrations of SS, Chl-a, turbidity and NO3-N in the culture tank water in RAS were significantly (Pculture without wetland treatment. However, no significant difference (Pculture environment, consequently increasing the shrimp growth and survival without water exchange, in a recirculating system. PMID:12663210

  18. HepaRG culture in tethered spheroids as an in vitro three-dimensional model for drug safety screening.

    PubMed

    Wang, Zenan; Luo, Xiaobei; Anene-Nzelu, Chukwuemeka; Yu, Yu; Hong, Xin; Singh, Nisha Hari; Xia, Lei; Liu, Side; Yu, Hanry

    2015-08-01

    Conventional two-dimensional (2D) monolayer cultures of HepaRG cells allow in vitro maintenance of many liver-specific functions. However, cellular dedifferentiation and functional deterioration over an extended culture period in the conventional 2D HepaRG culture have hampered its applications in drug testing. To address this issue, we developed tethered spheroids of HepaRG cells on Arg-Gly-Asp (RGD) and galactose-conjugated substratum with an optimized hybrid ratio as an in vitro three-dimensional (3D) human hepatocyte model. The liver-specific gene expression level and drug metabolizing enzyme activities in HepaRG-tethered spheorids were markedly higher than those in 2D cultures throughout the culture period of 7?days. The inducibility of three major cytochrome P450 (CYP) enzymes, namely CYP1A2, CYP2B6 and CYP3A4, was improved in both mRNA and activity level in tethered spheroids. Drug-induced cytotoxic responses to model hepatotoxins (acetaminophen, chlorpromazine and ketoconazole) in tethered spheroids were comparable to 2D cultures as well as other studies in the literature. Our results suggested that the HepaRG-tethered spheroid would be an alternative in vitro model suitable for drug safety screening. Copyright © 2014 John Wiley & Sons, Ltd. PMID:25512232

  19. Cultural Complexity

    Microsoft Academic Search

    Wolfgang Fikentscher

    1998-01-01

    This paper is divided into four parts. The first section (I.) consists of definitions which center on the concept of complexity. As a minimum, the terms, system, complexity, and adaptation as used in this paper need an explanation. The second section (II.) is a sketch of cultural complexity in the real world. I will use Pueblo tribal laws and other

  20. Hydroponic Culture

    ERIC Educational Resources Information Center

    Steucek, G. L.; Yurkiewicz, W. J.

    1973-01-01

    Describes a hydroponic culture technique suitable for student exercises in biology. This technique of growing plants in nutrient solutions enhances plant growth, and is an excellent way to obtain intact plants with root systems free of soil or other particulate matter. (JR)

  1. Culture of mature trophoblastic giant cells from bovine placentomes.

    PubMed

    Landim, L P; Miglino, M A; Pfarrer, C; Ambrosio, C E; Garcia, J M

    2007-04-01

    The mostly binucleate trophoblast giant cells (TGC) found in bovine placentomes, in addition to synthesizing and releasing hormones play an important role in fetal development and maternal adaptation to pregnancy. Placentomes from early gestation were collected, and for isolation of mature TGC, three cellular disaggregation methods, mechanical (MECH), enzymatic by trypsin (TRYP) or collagenase (COLL) were compared to each other. Further on, the cell survival in culture medium (DMEM) supplemented with either 10% fetal calf serum (FCS) or 10% serum replacement (SR) on culture plates free of any substrate was evaluated over a period of 90 days by trypan blue exclusion. The cells were further characterized by HOECHST 33342 nuclear staining, and immunocytochemical staining with monoclonal antibodies against vimentin and cytokeratin. A mean total rate of TGC survival of 82.56% was recorded. Statistical analysis showed significantly higher survival rates after enzymatic disaggregation with COLL (86.23%) than following MECH (80.38%) or TRYP (80.91%) treatment. Supplementation of DMEM with FCS resulted in significantly higher cellular survival rates (87.13%) when compared to the addition of SR (77.73%). Analysis of the influence of both, disaggregation method and medium supplementation on TGC survival revealed statistically significant differences between the following groups: MECH-SR (71.09%) was significantly lower than all other groups; TRYP-SR (78.03%) was significantly different from all other groups; TRYP-FCS (83.43%) and COLL-SR (84.08%) were significantly lower than MECH-FCS (89.98%) which together with COLL-FCS (88.25%) showed the highest cellular survival rate. In summary, our results show that TGC isolated from early gestation placentomes may be viable for more than 90 days of culture. However, whether these TGC produce placental lactogen throughout this period has yet to be determined. PMID:16716544

  2. Horticulture Day August 31, 2011

    E-print Network

    Dahlberg, E. Dan

    Horticulture Day Open House August 31, 2011 2:00--6:00 p.m. Join us on Wednesday, August 31, 2011, for our annual Horticulture Day Open House at the University of Minnesota North Central Research has been the site for world-class horticultural research for decades. A critical niche we fill

  3. In Defense of Snow Days

    ERIC Educational Resources Information Center

    Goodman, Joshua

    2015-01-01

    In snowy climates, school superintendents must frequently decide whether an impending storm warrants closing schools for the day. Concerns about student and teacher safety must be weighed against the loss of student learning time, along with state requirements for days of instruction and the cost and inconvenience of extending the school year into…

  4. Day Care Infection Control Protocol.

    ERIC Educational Resources Information Center

    Seattle-King County Dept. of Public Health, Seattle, WA.

    This day care infection control manual was assembled to provide technical guidance for the prevention and control of communicable diseases to child day care facilities in Seattle and King County, Washington. For each disease, the manual provides background information, public health control recommendations, and letters that can be used to…

  5. Day Care for America's Children.

    ERIC Educational Resources Information Center

    LaCrosse, E. Robert

    High quality day care is a pressing social need for the 1970's. Factors responsible for the strong interest in day care include pressures for welfare reform, the growing number of women in the labor force, minority pressures for equal opportunities, and research findings stressing the importance of development during the early years of a child's…

  6. 2014 Maine Earth Science Day

    USGS Multimedia Gallery

    On October 15, 2014 Maine Earth Science Day was held at the Maine State Museum in Augusta. The USGS was represented by Charlie Culbertson, left, and Nick Waldron, right. This photo was taken as the two were packing up for the day, and shows a main feature of the table, a touch screen display with th...

  7. Culture of human septal chondrocytes in a rotary bioreactor

    PubMed Central

    Reuther, Marsha S.; Briggs, Kristen K.; Wong, Van W.; Chang, Angela A.; Schumacher, Barbara L.; Masuda, Koichi; Sah, Robert L.; Watson, Deborah

    2014-01-01

    Objective 1) To show that extracellular matrix deposition is possible in 3-dimensional culture of human septal chondrocytes cultured in a rotary bioreactor as well as in static conditions. 2) To demonstrate that the biomechanical properties of human septal chondrocytes cultured in a bioreactor and static culture amplify with time. Study Design Prospective, basic science Setting Research laboratory Methods Human septal chondrocytes from nine donors were expanded in monolayer and seeded in alginate beads. The beads were cultured in a rotary bioreactor for 21 days in media supplemented with growth factors and human serum, using static culture as the control. Biochemical and biomechanical properties of the beads were measured. Results Glycosaminoglycan (GAG) accumulation significantly increased during two measured time intervals, zero to 10 days and 10 to 21 days (p<0.01). No significant difference was seen between the static and bioreactor conditions. However, substantial type II collagen production was demonstrated in the beads terminated at day 21 of culture in both conditions. In addition, the biomechanical properties of the beads were enhanced at 21 days in comparison to beads from days 0 and 10. Conclusion Human septal chondrocytes cultured in alginate beads exhibit significant matrix deposition and improved biomechanical properties after 21 days. Enhanced matrix deposition during bead culture will expectantly lead to formation of neocartilage that is comparable to native tissue. Matrix production in beads is supported by the use of a rotary bioreactor. PMID:22597575

  8. Culturing Conceptions: From First Principles

    ERIC Educational Resources Information Center

    Roth, Wolff-Michael; Lee, Yew Jin; Hwang, SungWon

    2008-01-01

    Over the past three decades, science educators have accumulated a vast amount of information on conceptions--variously defined as beliefs, ontologies, cognitive structures, mental models, or frameworks--that generally (at least initially) have been derived from interviews about certain topics. During the same time period, cultural studies has…

  9. Exploring Ancient World Cultures: An Introduction to Ancient World Cultures on the World-Wide Web

    NSDL National Science Digital Library

    Exploring Ancient World Cultures, provided by University of Evansville, "is an introductory, on-line, college-level 'textbook' of ancient world cultures, constructed around a series of cultural pages consisting of: The Ancient Near East, Ancient India, Ancient Egypt, Ancient China, Ancient Greece, Ancient Rome, Early Islam, and Medieval Europe." Each home page may contain essays by subject specialists, an anthology of readings from the period, a chronology, bibliographic resources, hypertext links to related sites, and computer graded quizzes. Interested users can also navigate the site by topic across cultures. A highlight of the site is the ability it gives the user to view the entire chronology, or to click on a year and culture and then another culture, in order to compare cross-cultural developments at the same time period. The site is a work in progress that promises maps and images in the near future.

  10. 'From Cultural Studies to Cultural Science

    Microsoft Academic Search

    John Hartley

    2009-01-01

    This paper, first presented at a symposium on the 'past, present and future of cultural studies,' traces disciplinary changes in the study of culture from the perspective of 'cultural science,' a term that was used by some of the earliest practitioners of cultural studies, including Raymond Williams. The paper goes on to describe some problems with cultural studies as it

  11. Manufacturing porcine islets: culture at 22°C has no advantage above culture at 37°C

    PubMed Central

    Mueller, Kate R; Martins, Kyra V; Murtaugh, Michael P; Schuurman, Henk-Jan; Papas, Klearchos K

    2013-01-01

    Background The manufacturing process of islets includes a culture step which was originally introduced to ease the logistics of procedures in preparing the graft and transplant recipient. It has been suggested that culture at room temperature has an advantage over culture at 37°C, in part by reducing immunogenicity via preferential elimination of contaminating cells (such as passenger leukocytes) within islets. We investigated this using islets isolated from pancreata of adult pigs. Methods Porcine islets were isolated from three donors and cultured at 37°C for 1 day, and then under three different conditions: 37°C for 6 days (condition A); 22°C for 6 days (condition B); or 22°C for 5 days followed by 37°C for 1 day (condition C). Recovery was assessed by DNA measurement, viability by oxygen consumption rate normalized for DNA (OCR/DNA), and gene expression by RT-PCR for a series of 9 lymphocyte markers, 11 lymphokines and chemokines, and 14 apoptotic and stress markers. Results Post-culture islet recoveries were similar for the three culture conditions. Average OCR/DNA values were 129–159 nmol/min.mgDNA before culture, and 259–291, 204–212, and 207–228 nmol/min•mgDNA, respectively, for culture under conditions A, B, and C, respectively. Irrespective of culture condition, examined gene expression in all three series of lymphocyte markers, lymphokines and chemokines, and apoptotic and stress markers manifested a statistically significant decrease upon culture for 7 days. This decrease was most dramatic for condition A: in particular most of lymphocyte markers showed a >10-fold reduction and also 6 markers in the lymphokine and chemokine series: these reductions are consistent with the elimination of immune cells present within islets during culture. The reduction was less for apoptotic and stress markers. For culture under condition B the reduction in gene expression was less, and culture under condition C resulted in gene expression levels similar to those under condition A: this indicates that 24 hours at 37°C is sufficient to re-equilibrate gene expression levels from those in islets cultured at 22°C to those in islets cultured at 37°C. Results were consistent among the preparations from the three donors. Conclusions Culture of porcine islets at 37°C provides benefits over culture at 22°C with respect to OCR/DNA outcomes and reduced expression of genes encoding lymphocyte markers, lymphokines and chemokines, and markers for apoptosis and stress. PMID:23941232

  12. Biomass production and nutrient removal potential of water hyacinth cultured in sewage effluent

    SciTech Connect

    Reddy, K.R.; Hueston, F.M.; McKim, T.

    1985-05-01

    Growth and nutrient uptake of water hyacinth (Eichhornia crassipes (Mart) Solms) cultured in sewage effluent were measured over a period of 1 year in a prototype wastewater treatment system which has been in operation at Walt Disney World near Orlando, Florida. Annual productivity of water hyacinth cultured in primary sewage effluent (Channel II) was found to be in the range of 5 to 27 g dry wt m/sup -2/ day/sup -1/ (23.6 dry tons acre/sup -1/ yr/sup -1/). Average growth rate during the months of May through October 1982 for hyacinth cultured in Channel II (primary sewage effluent) and Channel I (treated primary sewage effluent leaving Channel II) was about 16 g dry wt m/sup -2/ day/sup -1/ (27 dry tons acre/sup -1/ yr/sup -1/), compared to the growth rate of 13 g dry wt m/sup -2/ day/sup -1/ (22 dry tons acre/sup -1/ yr/sup -1/) for hyacinths cultured in secondary sewage effluent. Plants cultured in secondary sewage effluent generally had longer roots than the plants cultured in primary sewage effluent. A significant relationship was observed between the growth rate of hyacinth and the solar radiation. Nitrogen and P concentration of the plant tissue were higher in the hyacinths cultured during winter months compared to the plants grown in summer months. Average N and P concentration of the plants cultured in primary sewage effluent were found to be 3.7 percent N and 0.94 percent P, respectively, while the plants cultured in secondary sewage effluent had a total N and P content of 2.8 percent N and 0.79 percent P. Nutrient ratios of the major plant nutrients were found to be approximately the same as the nutrient ratios in the sewage effluent. Annual N and P uptake rates of hyacinth cultured in sewage effluent were found to be in the range of 1176 to 1193 kg N ha/sup -1/ yr/sup -1/ and 321 to 387 kg P ha/sup -1/ yr/sup -1/, respectively.

  13. The American Child: A Cultural Studies Reader.

    ERIC Educational Resources Information Center

    Levander, Caroline F., Ed.; Singley, Carol J., Ed.

    There has been increased critical interest in the child as a rich and varied site of cultural inscription. Scholars from a wide range of disciplines have turned their attention to the child in order to interrogate how it comes to represent, and often codify, the prevailing ideologies of a given culture or historical period. Noting that narratives…

  14. The day-to-day occurrence of equatorial plasma bubbles measured from Vanimo, Papua New Guinea

    NASA Astrophysics Data System (ADS)

    Carter, B. A.; Yizengaw, E.; Francis, M.; Terkildsen, M. B.; Marshall, R. A.; Norman, R.; Zhang, K.

    2013-12-01

    An analysis of the occurrence of Equatorial Plasma Bubbles (EPBs) detected using a ground-based Global Positioning System (GPS) receiver located at Vanimo in South-east Asia will be presented. The 3-year (2000-2002) dataset employed shows that the EPB occurrence maximizes (minimizes) during the equinoxes (solstices), in good agreement with previous findings. The low-latitude ionosonde station at Vanimo is used in conjunction with the GPS receiver in an analysis of the day-to-day EPB occurrence variability during the equinox period. A superposed epoch analysis of the ionosonde data reveals that the height, and the change in height, of the F layer is 1 standard deviation (1?) larger on the days for which EPBs were detected, compared to non-EPB days. These results are interpreted using the generalized Rayleigh-Taylor (R-T) plasma instability growth rate, for which stronger upward drift of the lower-altitude F-layer plasma promotes faster growth of EPBs after sunset. These results are then compared to the results of the Thermosphere Ionosphere Electrodynamic General Circulation Model (TIEGCM), which surprisingly show strong similarities to the observations, despite only using geomagnetic and solar activity inputs. The TIEGCM is also used to directly calculate the hourly flux-tube integrated R-T growth rate. A superposed epoch analysis reveals that the modeled R-T growth rate is a little less than 1? higher on average for EPB days compared to non-EPB days. The implication of this result is that the TIEGCM generates almost enough day-to-day variability in order to account for the day-to-day EPB occurrence observed during the equinox. This result isn't necessarily expected due to the model's limited altitude coverage of 100-700 km (depending on solar activity) and the lack of ionospheric observation inputs. It is thought that the remaining variability could originate from either lower altitudes (e.g. atmospheric gravity waves from the troposphere) or from higher altitudes (resulting from coupling with the magnetosphere and solar wind), or potentially both. It is concluded that the continuing advancement of numerical modeling of the thermosphere and ionosphere, coupled with altitudes above and below, is required to better understand the day-to-day EPB occurrence.

  15. Day-to-day repeatability of the Pulse Time Index of Norm

    PubMed Central

    Posokhov, Igor N; Konradi, Aleksandra O; Shlyakhto, Eugeny V; Mamontov, Oleg V; Orlov, Artemy V; Rogoza, Anatoly N

    2014-01-01

    The pulse wave velocity (PWV) threshold for hypertensive target organ damage is presently set at 10 meters per second. New 24-hour monitors (eg, BPLab® and Vasotens®) provide several PWV measurements over a period of 24–72 hours. A new parameter, ie, the Pulse Time Index of Norm (PTIN), can be calculated from these data. The PTIN is defined as the percentage of a 24-hour period during which the PWV does not exceed 10 meters per second. The aim of the present study was to test the new PTIN for clinical feasibility using day-to-day repeatability analysis. Oscillometrically generated waveform files (n=85), which were previously used for research studies, were reanalyzed using the new 2013 version software of the Vasotens technology program, which enables calculation of PTIN. The intraclass correlation coefficient was 0.98 and Cronbach’s alpha was 0.97, indicating that the PTIN has excellent day-to-day repeatability and internal consistency. The present results show adequate repeatability, and PTIN assessment using the Vasotens technology appears to be feasible. PMID:24600253

  16. Mesenchymal stem cells cultured under hypoxia escape from senescence via down-regulation of p16 and extracellular signal regulated kinase

    SciTech Connect

    Jin, Yonghui; Kato, Tomohisa; Furu, Moritoshi [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan)] [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan); Nasu, Akira [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan) [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan); Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University (Japan); Kajita, Yoichiro [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan) [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan); Department of Urology, Graduate School of Medicine, Kyoto University (Japan); Mitsui, Hiroto [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan) [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan); Department of Musculoskeletal Medicine, Graduate School of Medical Sciences, Nagoya City University (Japan); Ueda, Michiko [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan)] [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan); Aoyama, Tomoki [Human Health Sciences, Graduate School of Medicine, Kyoto University (Japan)] [Human Health Sciences, Graduate School of Medicine, Kyoto University (Japan); Nakayama, Tomitaka; Nakamura, Takashi [Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University (Japan)] [Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University (Japan); Toguchida, Junya, E-mail: togjun@frontier.kyoto-u.ac.jp [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan) [Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University (Japan); Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University (Japan); Center for iPS Cell Research and Application, Institute for Integrated Cell - Material Sciences, Kyoto University (Japan)

    2010-01-15

    Hypoxia has been considered to affect the properties of tissue stem cells including mesenchymal stem cells (MSCs). Effects of long periods of exposure to hypoxia on human MSCs, however, have not been clearly demonstrated. MSCs cultured under normoxic conditions (20% pO{sub 2}) ceased to proliferate after 15-25 population doublings, while MSCs cultured under hypoxic conditions (1% pO{sub 2}) retained the ability to proliferate with an additional 8-20 population doublings. Most of the MSCs cultured under normoxic conditions were in a senescent state after 100 days, while few senescent cells were found in the hypoxic culture, which was associated with a down-regulation of p16 gene expression. MSCs cultured for 100 days under hypoxic conditions were superior to those cultured under normoxic conditions in the ability to differentiate into the chondro- and adipogenic, but not osteogenic, lineage. Among the molecules related to mitogen-activated protein kinase (MAPK) signaling pathways, extracellular signal regulated kinase (ERK) was significantly down-regulated by hypoxia, which helped to inhibit the up-regulation of p16 gene expression. Therefore, the hypoxic culture retained MSCs in an undifferentiated and senescence-free state through the down-regulation of p16 and ERK.

  17. A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues.

    PubMed

    Aziz, Faisal; Yang, Xuesong; Wen, Qingping; Yan, Qiu

    2015-08-01

    At present, biopsy specimens, cancer cell lines and tissues obtained by gastric surgery are used in the study and analysis of gastric cancer, including the molecular mechanisms and proteomics. However, fibroblasts and other tissue components may interfere with these techniques. Therefore, the present study aimed to develop a procedure for the isolation of viable human gastric epithelial cells from gastric surgical tissues. A method was developed to culture human gastric epithelial cells using fresh, surgically excised tissues and was evaluated using immunocytochemistry, periodic acid?Schiff (PAS) staining and cell viability assays. Low cell growth was observed surrounding the gastric tissue on the seventh day of tissue explant culture. Cell growth subsequently increased, and at 12 days post?explant a high number of pure epithelial cells were detected. The gastric cancer cells exhibited rapid growth with a doubling time of 13?52 h, as compared to normal cells, which had a doubling time of 20?53 h. Immunocytochemical analyses of primary gastric cells revealed positive staining for cytokeratin 18 and 19, which indicated that the culture was comprised of pure epithelial cells and contained no fibroblasts. Furthermore, PAS staining demonstrated that the cultured gastric cells produced neutral mucin. Granulin and carbohydrate antigen 724 staining confirmed the purity of gastric cancer and normal cells in culture. This method of cell culture indicated that the gastric cells in primary culture consisted of mucin?secreting gastric epithelial cells, which may be useful for the study of gastric infection with Helicobacter pylori and gastric cancer. PMID:25937205

  18. Celtic Art and Cultures

    NSDL National Science Digital Library

    The Celtic Art and Cultures Website was originally created for art history students at University of North Carolina, Chapel Hill and can now be used by any would-be student of Celtic Art with access to a newer Web browser. The main feature of the site is its multimedia database of Celtic-related images, maps, timelines, and vocabulary aids complete with spoken pronunciations. Images can be viewed by period, material, object, and country. If you find yourself uncertain of which time period or object type to choose, as I did, helpful information is available in the Maps & Timelines section. The course syllabus, which can be found under site info, also provides an overview of Celtic Art.

  19. Study on tissue culture for Gelidium seedling

    NASA Astrophysics Data System (ADS)

    Pei, Lu-Qing; Luo, Qi-Jun; Fei, Zhi-Qing; Ma, Bin

    1996-06-01

    As seedling culture is a crucial factor for successful cultivation of Gelidium, the authors researched tissue culture technology for producing seedlings. The morphogeny and experimental ecology were observed and studied fully in 2 5 mm isolated tissue fragments. Regeneration, appearance of branching creepers and attaching structure and new erect seedlings production and development were studied. Fragments were sown on bamboo slice and vinylon rope. The seedlings were cultured 20 30 days indoor, then cultured in the sea, where the density of erect seedlings was 3 19 seedlings/cm2, growth rate was 3.84% day. The frond arising from seedlings directly was up to 10 cm per year. The ecological conditions for regenerated seedlings are similar to the natural ones. The regenerated seedlings are suitable for raft culture in various sea areas.

  20. Periodicities in the IUE particle radiation data

    NASA Astrophysics Data System (ADS)

    Arquilla, Richard

    1993-06-01

    The magnetospheric charged particle flux measured serendipitously by the IUE satellite during perigee passages from 1978 February to 1989 February has been searched for periodicities using Fourier transform and correlation techniques. The analysis uncovers persistent modulations of the particle flux with periods of 28-33 days and 15-17 days. The particle flux seen by IUE in the outer van Allen belt is modulated to a significant extent by the magnetosphere's interaction with high-speed solar wind streams from coronal holes, in agreement with analyses of fluxes at other altitudes and of geomagnetic disturbance indices. It is suggested that recurrent global solar-surface activity, such as the 25.8-day periodicity discussed by Bai and Sturrock (1991) and the solar 'hot spots' reported by Bai (1987, 1988, 1990), may play a role in modulating the particle flux near solar maximum.

  1. The ocean sampling day consortium.

    PubMed

    Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-Guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Gerdts, Gunnar; Polymenakou, Paraskevi; Kotoulas, Giorgos; Siam, Rania; Abdallah, Rehab Z; Sonnenschein, Eva C; Cariou, Thierry; O'Gara, Fergal; Jackson, Stephen; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Caçador, Isabel; Canning-Clode, João; Bobrova, Oleksandra; Marteinsson, Viggo; Reynisson, Eyjolfur; Loureiro, Clara Magalhães; Luna, Gian Marco; Quero, Grazia Marina; Löscher, Carolin R; Kremp, Anke; DeLorenzo, Marie E; Øvreås, Lise; Tolman, Jennifer; LaRoche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P; Ramos, Sandra; Magalhães, Catarina; Jude-Lemeilleur, Florence; Aguirre-Macedo, Ma Leopoldina; Wang, Shiao; Poulton, Nicole; Jones, Scott; Collin, Rachel; Fuhrman, Jed A; Conan, Pascal; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Yakimov, Michael M; Baltar, Federico; Bodrossy, Levente; Van De Kamp, Jodie; Frampton, Dion Mf; Ostrowski, Martin; Van Ruth, Paul; Malthouse, Paul; Claus, Simon; Deneudt, Klaas; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C; Kandil, Mahrous M; Amaral, Valentina; Biancalana, Florencia; Santana, Rafael; Pedrotti, Maria Luiza; Yoshida, Takashi; Ogata, Hiroyuki; Ingleton, Tim; Munnik, Kate; Rodriguez-Ezpeleta, Naiara; Berteaux-Lecellier, Veronique; Wecker, Patricia; Cancio, Ibon; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Zaid, El Houcine; Boukhatem, Noureddine; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Todorova, Nadezhda; Karamfilov, Ventzislav; Ten Hoopen, Petra; Cochrane, Guy; L'Haridon, Stephane; Bizsel, Kemal Can; Vezzi, Alessandro; Lauro, Federico M; Martin, Patrick; Jensen, Rachelle M; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; De Pascale, Fabio; Schiavon, Riccardo; Dos Santos, Antonina; Villar, Emilie; Pesant, Stéphane; Cataletto, Bruno; Malfatti, Francesca; Edirisinghe, Ranjith; Silveira, Jorge A Herrera; Barbier, Michele; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut Cerkez; Bresnan, Eileen; Iriberri, Juan; Nyhus, Paul Anders Fronth; Bente, Edvardsen; Karlsen, Hans Erik; Golyshin, Peter N; Gasol, Josep M; Moncheva, Snejana; Dzhembekova, Nina; Johnson, Zackary; Sinigalliano, Christopher David; Gidley, Maribeth Louise; Zingone, Adriana; Danovaro, Roberto; Tsiamis, George; Clark, Melody S; Costa, Ana Cristina; El Bour, Monia; Martins, Ana M; Collins, R Eric; Ducluzeau, Anne-Lise; Martinez, Jonathan; Costello, Mark J; Amaral-Zettler, Linda A; Gilbert, Jack A; Davies, Neil; Field, Dawn; Glöckner, Frank Oliver

    2015-01-01

    Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world's oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits. PMID:26097697

  2. National Mole Day Foundation, Inc.

    NSDL National Science Digital Library

    "Celebrated annually on October 23 from 6:02 a.m. to 6:02 p.m., Mole Day commemorates Avogadro's Number (6.02 x 10^23), which is a basic measuring unit in chemistry." This website presents the National Mole Day Foundation, Inc.'s actions to get everyone, especially children, excited about chemistry. Users can learn about activities held throughout the United States and the world including the next Mole day breakfast held on April 3, 2004. The website features many awards to worthy teachers and schools. Interested teachers and students can learn how to become involved in this fun, educational organization.

  3. ET culture

    Microsoft Academic Search

    Debbora Battaglia

    This chapter invites the reader to enter the outerspaces of extraterrestrial culture, as a realm of social inquiry. Where\\u000a this journey leads is perhaps unexpected, especially for the discourse of aliens and Unidentified Flying Objects (UFOs). For\\u000a while we might expect to engage fields of exotic Otherness — of technomarvels and bizarre entities, epic enterprises, and\\u000a terrors unrecognizable in their

  4. [Neonatal palliative care and culture].

    PubMed

    Bétrémieux, P; Mannoni, C

    2013-09-01

    The period of palliative care is a difficult time for parents and caregivers because they are all weakened by the proximity of death. First of all, because of religious and cultural differences, parents and families cannot easily express their beliefs or the rituals they are required to develop; second, this impossibility results in conflicts between the caregiver team and the family with consequences for both. Caregivers are concerned to allow the expression of religious beliefs and cultural demands because it is assumed that they may promote the work of mourning by relating the dead child to its family and roots. However, caregivers' fear not knowing the cultural context to which the family belongs and having inappropriate words or gestures, as sometimes families dare not, cannot, or do not wish to describe their cultural background. We attempt to differentiate what relates to culture and to religion and attempt to identify areas of potential disagreement between doctors, staff, and family. Everyone has to work with the parents to open a space of freedom that is not limited by cultural and religious assumptions. The appropriation of medical anthropology concepts allows caregivers to understand simply the obligations imposed on parents by their culture and/or their religion and open access to their wishes. Sometimes help from interpreters, mediators, ethnopsychologists, and religious representatives is needed to understand this reality. PMID:23896086

  5. Retrovirus-mediated gene transfer in primary T lymphocytes impairs their anti-Epstein-Barr virus potential through both culture-dependent and selection process-dependent mechanisms

    Microsoft Academic Search

    Delphine Sauce; Marie Bodinier; Marina Garin; Bruno Petracca; Nicolas Tonnelier; Anne Duperrier; Junia V. Melo; Jane F. Apperley; Christophe Ferrand; Patrick Herve; Francois Lang; Pierre Tiberghien; Eric Robinet

    2002-01-01

    To modulate alloreactivity after hemato- poietic stem cell transplantation, suicide gene-expressing donor T cells can be administered with an allogeneic T-cell- depleted bone marrow graft. Immune com- petence of such cells is a critical issue. The impact of the ex vivo gene transfer protocol (12-day culture period including CD3\\/interleukin-2 (IL-2) activation, retrovi- ral-mediated gene transfer, and G418- based selection) on

  6. Valentine's Day Party Weekday Activities

    E-print Network

    Pilyugin, Sergei S.

    Valentine's Day desserts. Feel free to bring food like cupcakes, cookies, or candy to share with everyone hard; hard boiled; soft boiled; poached (broken open and cooked in a little bit of water); and various

  7. Today's Date_______/________/________ (Month) (Day) (Year)

    E-print Network

    Massachusetts at Amherst, University of

    Today's Date_______/________/________ (Month) (Day) (Year) Private Swim Instruction Fees/Packages **Registrant must have current recreation membership to participate in private swim lessons** 1 Lesson $25 3_____________________________________________________________________________________ (Last) (First) (MI) UCard# __________________________________________ Age________________ Male Female

  8. Career Day - Duration: 62 seconds.

    NASA Video Gallery

    NASA's 2013 Career Days was a joint collaboration between NASA Langley and the Newport News Shipbuilding where 600 high school students from Virginia took on two design challenges -- designing a ca...

  9. First Year: Clear, cold day

    E-print Network

    Chapman, Michael S.

    First Year: A Gap [I] Clear, cold day Girl sitting on the corner Pile of stained napkins Bright red is invading my brain But Somewhere between the Knowing and Helping There is a gap. By Julia Armendariz, MS1

  10. Last Days of Life (PDQ®)

    Cancer.gov

    Expert-reviewed information summary about care during the last days to last hours of life, including common symptoms, ethical dilemmas that may arise, and the role of the oncologist in caring for patients and their families during this time.

  11. DISTINGUISHED AWARD FOUNDERS DAY 2012

    E-print Network

    Provancher, William

    _______________________ E-mail Address ____________________________ U of U Degree(s), Major(s), Class Year_____________________________________________________________ E-mail Address_____________________________________________________________ Supporting Information. Presentation of the awards will take place at the Founders Day Banquet, held on February 29, 2012. Please send

  12. Question of the Day Examples

    NSDL National Science Digital Library

    Refine the Results Biosphere 2 matches Climate 2 matches Earth surface 1 match Ocean 4 matches Solid Earth 2 matches Results 1 - 10 of 11 matches Question of the Day: Efficiency of Food Production part of Question ...

  13. STS-91 Day 04 Highlights

    NASA Technical Reports Server (NTRS)

    1998-01-01

    On this forth day of the STS-91 mission, the flight crew, Cmdr. Charles J. Precourt, Pilot Dominic L. Pudwill Gorie and Mission Specialists Wendy B. Lawrence, Franklin R. Chang-Diaz, Janet L. Kavandi, and Valery Victorovitch Ryumin are awakened to the sounds of 'South Australia,' honoring Thomas who is a native of Adelaide in South Australia. The nine astronauts and cosmonauts aboard Discovery-Mir are spending their first full day of joint operations continuing the transfer of about four tons of logistical supplies and equipment. Much of the day is spent transferring water, scientific gear and other hardware between the two spacecraft. The crew members had transferred five bags of water to the Mir by the end of the day.

  14. Reading Period Resolution Sponsors: Zachary Malter `13, Mari Jarris `14, Sam Ebb `13

    E-print Network

    Royer, Dana

    Reading Period Resolution Sponsors: Zachary Malter `13, Mari Jarris `14, Sam Ebb `13 Recognizing continuing the practice of 4 days of finals and 4 days of reading period or expanding reading period, Bearing of reading period, especially for advanced courses that require long final assignments", Aware

  15. Astronomy Picture of the Day

    NSDL National Science Digital Library

    Each day a different image or photograph of a celestial object is featured. Pictures are selected for their particular beauty or astronomical interest, and each one is accompanied by a brief caption written by a professional astronomer. Links to additional information are embedded in the text of the caption. Previous pictures of the day are archived in reverse chronological order dating back to June 1995.

  16. Mouse bone marrow-derived mast cells (BMMC) change their phenotype when cultured with fibroblasts

    SciTech Connect

    Levi-Schaffer, F.; Austen, K.F.; Stevens, R.L.

    1986-03-05

    The heparin-containing mast cells (HP-MC) that reside in the connective tissues of the mouse, but not the chondroitin sulfate containing mast cells in the gastrointestinal mucosa, stain with safranin when exposed to alcian blue/safranin. Mouse BMMC (the presumptive in vitro counterpart of the in vivo differentiated mucosal mast cell) were cultured for 2-14 days with confluent skin-derived 3T3 fibroblasts in RPMI-1640 containing 10% fetal calf serum and 50% WEHI-3 conditioned medium. Although the BMMC adhered to the fibroblast monolayer, they continued to divide, probably due to the presence of interleukin-3 in the conditioned medium. The mast cells remained viable throughout the period of co-culture, since they failed to release LDG and because they increased their histamine content per cell approx.15-fold. After 8-9 days of co-culture, >50% of the BMMC changed histochemically becoming safranin positive. At this time, 30-50% of the (/sup 35/S)glycosaminoglycans on the proteoglycans synthesized by these co-cultured mass cells were heparin, whereas the initial BMMC synthesized proteoglycans containing only chondroitin sulfate E. That interleukin 3-dependent mouse BMMC can be induced to undergo a phenotypic change so as to express characteristics of a HP-MC suggests that the tissue microenvironment determines the differentiated characteristics of these cells.

  17. Immobilization of primary cultures of insulin-releasing human pancreatic cells.

    PubMed

    Mantovani, Marluce da Cunha; da Conceição, Mateus Meneghesso; Ferreira, Ari José Scattone; Labriola, Letícia; dos Santos, Patrícia Barros; Tonso, Aldo; Pereira, Carlos Augusto; El-Dorry, Hamza; Sogayar, Mari Cleide

    2009-01-01

    Transplantation of pancreatic islets isolated from organ donors constitutes a promising alternative treatment for type1 Diabetes, however, it is severely limited by the shortage of organ donors. Ex-vivo islet cell cultures appear as an attractive but still elusive approach for curing type 1 Diabetes. It has recently been shown that, even in the absence of fibrotic overgrowth, several factors, such as insufficient nutrition of the islet core, represent a major barrier for long-term survival of islets grafts. The use of immobilized dispersed cells may contribute to solve this problem due to conceivably easier nutritional and oxygen support to the cells.  Therefore, we set out to establish an immobilization method for primary cultures of human pancreatic cells by adsorption onto microcarriers (MCs). Dispersed human islets cells were seeded onto Cytodex1 microcarriers and cultured in bioreactors for up to eight days. The cell number increased and islet cells maintained their insulin secretion levels throughout the time period studied. Moreover, the cells also presented a tendency to cluster upon five days culturing.  Therefore, this procedure represents a useful tool for controlled studies on islet cells physiology and, also, for biotechnological applications. PMID:21099276

  18. Abiotic elicitation of gymnemic acid in the suspension cultures of Gymnema sylvestre.

    PubMed

    Ch, Bhuvaneswari; Rao, Kiranmayee; Gandi, Suryakala; Giri, Archana

    2012-02-01

    Elicitation is one of the few strategies that find commercial application in the enhancement of secondary metabolite production from plants as well as cell culture systems. Due to their immense medicinal value, production of saponins in suspension cultures has been attempted by many researchers. Gymnema sylvestre is a rich source of gymnemic acids (saponins) that find application in the treatment of diabetes. The present study is an attempt to evaluate the effect of various metal salts (cadmium chloride, mercuric chloride, silver nitrate, cupric chloride, cobaltous chloride and calcium chloride) in eliciting the response from G. sylvestre suspension cultures. The maximum gymnemic acid production in the suspensions was achieved on day 12 of culture, though the maximum biomass was obtained on day 16. Among the different salts, CdCl(2) gave maximum response (59.97 mg/gDCW) at 2 mM concentration after a 24 h time period, while, AgNO(3) gave the least response (18.35 mg/gDCW) on incubation of 48 h at 1 mM concentration, in terms of gymnemic acid accumulation. The accumulation of gymnemic acid was found to be dependent on treatment time and concentration of the elicitor. The enhanced gymnemic acid production shown by the suspensions in response to the metal salts indicates their role in evoking the plant defense mechanisms. These elicitation studies help in providing a platform for improved commercial supply of bioactive gymnemic acids. PMID:22806870

  19. Three-dimensional primary hepatocyte culture in synthetic self-assembling peptide hydrogel.

    PubMed

    Wang, Sihong; Nagrath, Deepak; Chen, Pohun C; Berthiaume, François; Yarmush, Martin L

    2008-02-01

    Drug metabolism studies and liver tissue engineering necessitate stable hepatocyte cultures that express liver functions for a minimum of 4 days to 3 weeks. Current techniques, using different biomaterials and geometries, that maintain hepatocellular function in vitro exhibit a low cell density and functional capacity per unit volume. Herein we investigated a well-defined synthetic peptide that can self-assemble into three-dimensional interweaving nanofiber scaffolds to form a hydrogel, PuraMatrix, as a substrate for hepatocyte culture. Freshly isolated primary rat hepatocytes attached, migrated, and formed spheroids within 3 days after seeding on PuraMatrix. Hepatocytes expressed the apical membrane marker dipeptidyl peptidase IV at cell-cell contacts. Compared to the collagen sandwich, albumin and urea secretion on PuraMatrix were higher for the first week, and cytochrome P450IA1 activity was higher throughout the culture period. Mitochondrial membrane potential 1 day after seeding was higher on PuraMatrix than in the collagen sandwich, suggesting better preservation of the metabolic machinery. PuraMatrix and Matrigel showed similar albumin and urea production. PuraMatrix is an attractive system for generating hepatocyte spheroids that quickly restore liver functions after seeding. This system is also amenable to scale-up, which makes it suitable for in vitro toxicity, hepatocyte transplantation, and bioartificial liver development studies. PMID:18333775

  20. One Day Every 216 Years, Three Days Each Decan. Rebirth Cycle of Pythagoras, Phoenix, Hazon Gabriel, and Christian Dogma of Resurrection Can Be Explained by the Metonic Cycle

    NASA Astrophysics Data System (ADS)

    Rothwangl, S.

    2009-08-01

    This article explains how the Metonic cycle is at the base of the period of 216 years Pythagoras believed in being reborn after that period. It shows how this period calendrically is related to other mythological worldviews such as the Phoenix myth, the Hebrean Hazon Gabriel, and the Christian dogma of resurrection on the third day.

  1. Periodicities of solar irradiance and solar activity indices

    Microsoft Academic Search

    Judit Pap; S. David Bouwer; W. Kent Tobiska

    1990-01-01

    The present FFT time-series analysis indicates the presence of an 8-11 month periodicity in solar total and UV irradiances, sunspot-blocking function, 10.7-cm radio flux, and Ca-K plage index. While the 51-day period is the dominant one in the projected areas of developing complex sunspot groups, it is missing from the old, decaying sunspot areas, thereby suggesting that the 51-day period

  2. On the Quarantine Period for Ebola Virus

    PubMed Central

    Haas, Charles N.

    2014-01-01

    Background: 21 days has been regarded as the appropriate quarantine period for holding individuals potentially exposed to Ebola Virus (EV) to reduce risk of contagion. There does not appear to be a systematic discussion of the basis for this period. Methods: The prior estimates for incubation time to EV were examined, along with data on the first 9 months of the current outbreak. These provided estimates of the distribution of incubation times. Results: A 21 day period for quarantine may result in the release of individuals with a 0.2 - 12% risk of release prior to full opportunity for the incubation to proceed. It is suggested that a detailed cost-benefit assessment, including considering full transmission risks, needs to occur in order to determine the appropriate quarantine period for potentially exposed individuals. PMID:25642371

  3. Volunteer Day Class Photo -Reminder

    E-print Network

    Pilyugin, Sergei S.

    to the homeless and interact with English speakers from different cultures. Meet at Norman Garage at 12:30pm're a veteran Fall C student, please welcome the new Fall B students. Immunizations & Insurance: All of our students are required to have certain immunizations and adequate health insurance in order to attend class

  4. A Day at the Museums

    ERIC Educational Resources Information Center

    Hubel, Joy Alter

    2009-01-01

    The school field trip, once a supporting player in a well-rounded education, is slowly becoming endangered. Widespread budget cuts have made happily anticipated class trips to museums, zoos, and other cultural destinations increasingly scarce. A librarian may be able to rescue the field trip from extinction by transforming the school building into…

  5. Galileo Teacher Training Program - GTTP Days

    NASA Astrophysics Data System (ADS)

    Heenatigala, T.; Doran, R.

    2012-09-01

    Despite the vast availability of teaching resources on the internet, finding a quality and user-friendly materials is a challenge. Many teachers are not trained with proper computing skills to search for the right materials. With years of expertise training teachers globally, Galileo Teacher Training Program (GTTP) [1] recognize the need of having a go-to place for teachers to access resources. To fill this need GTTP developed - GTTP Days - a program creating resource guides for planetary, lunar and solar fields. Avoiding the imbalance in science resources between the developed and undeveloped world, GTTP Days is available both online and offline as a printable version. Each resource guide covers areas such as scientific knowledge, exploration, observation, photography, art & culture and web tools. The lesson plans of each guide include hands-on activities, web tools, software tools, and activities for people with disabilities [2]. Each activity indicate the concepts used, the skills required and age level which guides the teachers and educators to select the correct content suitable for local curriculum.

  6. Role of water-soluble matrix fraction, extracted from the nacre of Pinctada maxima, in the regulation of cell activity in abalone mantle cell culture (Haliotis tuberculata).

    PubMed

    Sud, D; Doumenc, D; Lopez, E; Milet, C

    2001-04-01

    In mollusks, the mantle is responsible for the secretion of an organic matrix that mineralizes to form the shell. A model of mantle cell culture has been established from the nacreous gastropod Haliotis tuberculata. First, viability of cells, quantified by the MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide) reduction assay, was monitored in order to determine a cell density and a time-culturing period in order to investigate biomineralization processes in vitro. During the first 11 days of culture, an increase of MTT response demonstrated an activation of cultured cells mitochondrial activity as confirmed by the total protein content assay. The effect of a water-soluble extract from the organic matrix of Pinctada maxima (WSM) was tested on this cell culture system for 11 days-period exposure. WSM reduced the global viability of mantle cells in a dose-dependent way which corresponded to a cell death. Alkaline phosphatase activity normalized to total protein content increased in the presence of WSM. This increase may be due to an activation of cells and a selection of one (or a few) cell type(s). Further investigations will help us to determine this selectivity issue. PMID:11392668

  7. Protoplast culture and plant regeneration in Lycium barbarum L

    Microsoft Academic Search

    Yakov I. Ratushnyak; Nickolai M. Piven; Vladimir A. Rudas

    1989-01-01

    Protoplasts were isolated from leaves of the woody plant Lycium barbarum L. and cultured in liquid nutrient medium TM-2 at a density of 104–105 cells ml-1. After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5–7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after 30–40 days. Completely formed

  8. Rotation Period of Asteroid 4727 Ravel

    NASA Astrophysics Data System (ADS)

    Ergashev, K. E.; Ehgamberdiev, Sh. A.; Burkhonov, O. A.; Yoshida, Fumi

    2014-04-01

    The results of photometric observations of the main-belt asteroid 4727 Ravel (1979 UD1) from 2013 August 13- 16 at the Maidanak Astronomical Observatory of the Ulugh Beg Astronomical Institute (UBAI), Uzbekistan Academy of Sciences are presented. During the period of observations, the lightcurve amplitude was 0.32 ± 0.05 mag and the synodic rotation period was 0.185 ± 0.009 days (4.44 ± 0.22 h).

  9. Day-to-day variability of equatorial and low latitude F-region ionosphere in the Indian zone

    Microsoft Academic Search

    R. S. Dabas; Neerja Sharma; M. G. K. Pillai; A. K. Gwal

    2006-01-01

    The F-region variability of the ionosphere during high solar activity periods have been studied using different hourly foF2 data from Indian stations, Thiruvananthapuram (8.5°N, 76.8°E), Kodaikanal (10.2°N, 77.5°E) near the Dip equator and Delhi (28.6°N, 77.2°E) located north of the equatorial ionization anomaly (EIA). During the study three types of changes have been observed, viz., single day abnormality, alternate day

  10. Scripture-Based Discourses of Latter-Day Saint and Methodist Youths

    ERIC Educational Resources Information Center

    Rackley, Eric D.

    2014-01-01

    Drawing on social and cultural perspectives of literacy, conceptualizations of religious literacies, and Gee's notion of Discourses, I develop a framework for exploring 16 Latter-day Saint and Methodist youths' religious literacies as social and cultural practice. This work grows out of the increasingly important role that religion plays…

  11. Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture.

    PubMed

    Cheng, Boran; He, Zhaobo; Zhao, Libo; Fang, Yuan; Chen, Yuanyuan; He, Rongxiang; Chen, Fangfang; Song, Haibin; Deng, Yuliang; Zhao, Xingzhong; Xiong, Bin

    2014-01-01

    Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a "liquid biopsy" and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright's stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies. PMID:24904216

  12. Responses of the lower thermospheric temperature to the 9 day and 13.5 day oscillations of recurrent geomagnetic activity

    NASA Astrophysics Data System (ADS)

    Jiang, Guoying; Wang, Wenbin; Xu, Jiyao; Yue, Jia; Burns, Alan G.; Lei, Jiuhou; Mlynczak, Martin G.; Rusell, James M.

    2014-06-01

    Responses of the lower thermospheric temperature to the 9 day and 13.5 day oscillations of recurrent geomagnetic activity and solar EUV radiation have been investigated using neutral temperature data observed by the TIMED/SABER (Thermosphere Ionosphere Mesosphere Energetics and Dynamics/Sounding of the Atmosphere using Broadband Emission Radiometry) instrument and numerical experiments by the NCAR-TIME-GCM (National Center for Atmospheric Research-thermosphere-ionosphere-mesosphere electrodynamics-general circulation model). The TIMED/SABER data analyzed were for the period from 2002 to 2007 during the declining phase of solar cycle 23. The observations show that the zonal mean temperature in the lower thermosphere oscillated with periods of near 9 and 13.5 days in the height range of 100-120 km. These oscillations were more strongly correlated with the recurrent geomagnetic activity than with the solar EUV variability of the same periods. The 9 day and 13.5 day oscillations of lower thermospheric temperature had greater amplitudes at high latitudes than at low latitudes; they also had larger amplitudes at higher altitudes, and the oscillations could penetrate down to ~105 km, depending on the strength of the recurrent geomagnetic activity for a particular time period. The data further show that the periodic responses of the lower thermospheric temperature to recurrent geomagnetic activity were different in the two hemispheres. In addition, numerical experiments have been carried out using the NCAR-TIME-GCM to investigate the causal relationship between the temperature oscillations and the geomagnetic activity and solar EUV variations of the same periods. Model simulations showed the same periodic oscillations as those seen in the observations when the real geomagnetic activity index, Kp, was used to drive the model. These numerical results show that recurrent geomagnetic activity is the main cause of the 9 day and 13.5 day variations in the lower thermosphere temperature, and the contribution from solar EUV variations is minor. Furthermore, we also found that consecutive coronal mass ejection events could cause long-duration enhancements in the lower thermospheric temperature that strengthen the 9 day and 13.5 day signals, and this kind of phenomenon mostly occurred between 2002 and 2005 during the declining phase of solar cycle 23.

  13. Responses of the lower thermospheric temperature to the 9 day and 13.5 day oscillations of recurrent geomagnetic activity

    NASA Astrophysics Data System (ADS)

    Jiang, Guoying; Wang, Wenbin; Xu, Jiyao; Yue, Jia; Burns, Alan G.; Lei, Jiuhou; Mlynczak, Martin G.; Rusell, James M., III

    2015-04-01

    Responses of the lower thermospheric temperature to the 9 day and 13.5 day oscillations of recurrent geomagnetic activity and solar EUV radiation have been investigated using neutral temperature data observed by the TIMED/SABER (Thermosphere IonosphereMesosphere Energetics and Dynamics/Sounding of the Atmosphere using Broadband Emission Radiometry) instrument and numerical experiments by the NCAR-TIME-GCM (National Center for Atmospheric Research-thermosphere-ionosphere-mesosphere electrodynamics-general circulation model). The TIMED/SABER data analyzed were for the period from 2002 to 2007 during the declining phase of solar cycle 23. The observations show that the zonal mean temperature in the lower thermosphere oscillated with periods of near 9 and 13.5 days in the height range of 100-120 km. These oscillations were more strongly correlated with the recurrent geomagnetic activity than with the solar EUV variability of the same periods. The 9 day and 13.5 day oscillations of lower thermospheric temperature had greater amplitudes at high latitudes than at low latitudes; they also had larger amplitudes at higher altitudes, and the oscillations could penetrate down to ~105 km, depending on the strength of the recurrent geomagnetic activity for a particular time period. The data further show that the periodic responses of the lower thermospheric temperature to recurrent geomagnetic activity were different in the two hemispheres. In addition, numerical experiments have been carried out using the NCAR-TIME-GCM to investigate the causal relationship between the temperature oscillations and the geomagnetic activity and solar EUV variations of the same periods. Model simulations showed the same periodic oscillations as those seen in the observations when the real geomagnetic activity index, Kp, was used to drive the model. These numerical results show that recurrent geomagnetic activity is the main cause of the 9 day and 13.5 day variations in the lower thermosphere temperature, and the contribution from solar EUV variations is minor. Furthermore, we also found that consecutive coronal mass ejection events could cause long-duration enhancements in the lower thermospheric temperature that strengthen the 9 day and 13.5 day signals, and this kind of phenomenon mostly occurred between 2002 and 2005 during the declining phase of solar cycle 23.

  14. Culture conditions for individual plasmid preparations (Adapted from Open Biosystems).

    E-print Network

    Richardson, David

    should be used to culture pSM2 constructs. An incubation period of 14­18 hours at 37°C with aeration typically is sufficient. Receiving clones cultures from the RNAi Facility Upon receiving notice that clones high-purity and low endotoxin levels (Qiagen Catalog #12123). For pSM2 constructs, 5ml of culture

  15. Asymmetric adaptive legal cultures amongst minorities and Euro-migrants

    Microsoft Academic Search

    W. L. Miller

    2012-01-01

    In the post-communist period, there has been considerable pressure towards a more convergent pan-European legal culture. Under international pressure, formal laws can be changed, but legal cultures that include public perceptions, attitudes, habits, conventions and even behaviour may be resistant to change imposed on them from outside. Formal laws may be changed, but legal cultures still reflect the legacy of

  16. Flies Remember the Time of Day.

    PubMed

    Chouhan, Nitin S; Wolf, Reinhard; Helfrich-Förster, Charlotte; Heisenberg, Martin

    2015-06-15

    The circadian clock enables organisms to anticipate daily environmental cycles and drives corresponding changes in behavior [1, 2]. Such endogenous oscillators also enable animals to display time-specific memory [1, 3-5]. For instance, mice and honeybees associate the location of a stimulus (like food or mate) with a certain time of day (time-place learning) [6, 7]. However, the mechanism underlying time-related learning and memory is not known. In the present study, we investigate time-specific odor learning. We use a genetically tractable animal, the fly Drosophila melanogaster. Starved flies are trained in the morning and afternoon to associate distinct odors with sucrose reward. The training is repeated the next day, and their time-dependent odor preference is tested on the third day. Our results indicate that Drosophila can express appetitive memory at the relevant time of day if the two conditioning events are separated by more than 4 hr. Flies can form time-odor associations in constant darkness (DD) as well as in a daily light-dark (LD) cycle, but not when kept under constant light (LL) conditions. Circadian clock mutants, period(01) (per(01)) and clock(AR) (clk(AR)), learned to associate sucrose reward with a certain odor but were unable to form time-odor associations. Our findings show that flies can utilize temporal information as an additional cue in appetitive learning. Time-odor learning in flies depends on a per- and clk-dependent endogenous mechanism that is independent of environmental light cues. PMID:26028434

  17. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...2012-07-01 2012-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  18. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...2014-07-01 2014-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  19. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...2013-07-01 2013-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  20. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...2010-07-01 2010-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  1. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...2014-07-01 2014-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  2. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...2012-07-01 2012-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  3. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...2013-07-01 2013-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  4. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...2011-07-01 2011-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  5. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...2010-07-01 2010-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  6. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...2011-07-01 2011-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  7. Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture.

    PubMed

    Huang, Guo-Shiang; Tseng, Ting-Chen; Dai, Niann-Tzyy; Fu, Keng-Yen; Dai, Lien-Guo; Hsu, Shan-Hui

    2015-10-01

    Adipose-derived adult stem cells (ASCs) have gained much attention because of their multipotency and easy access. Here we describe a novel chitosan-based selection (CS) system instead of the conventional plastic adherence (PA) to obtain the primary ASCs. The minimal amount of adipose tissue for consistent isolation of ASCs is reduced from 10 mL to 5 mL. The selection is based on the specific interaction between cells and chitosan materials, which separate ASCs by forming spheroids during primary culture. The primary culture period was reduced from 4 days to one day and more ASCs (ten-fold expansion) were achieved in a week. The average duration for obtaining 1 × 10(7) cells takes about seven days from 5 mL of adipose tissue, compared to 14 days using the conventional PA method from 10 mL of adipose tissue. The replicative senescence of CS-ASCs is not evident until the fifteenth passage (vs. eighth for the PA-ASCs). The obtained ASCs (CS-ASCs) have less doubling time for the same passage of cells and show greater stemness than those obtained from the conventional PA method (PA-ASCs). Moreover, CS-ASCs undergo trilineage differentiation more effectively than PA-ASCs. The greater differentiation potential of CS-ASCs may be associated with the enrichment and maintenance of CD271 positive cells by chitosan selection of primary culture. PMID:26162932

  8. Starting a Day Care Center: The Day Care Center Handbook.

    ERIC Educational Resources Information Center

    Checkett, Donald

    Designed to be of help to individuals and groups seeking to establish a day care center in the metropolitan St. Louis area, this manual calls attention to important and basic information which must be taken into account if planning is to produce tangible results. Following a brief section defining commonly used terms referring to organized…

  9. A NMR metabolomics study of the ripening process of the Fiore Sardo cheese produced with autochthonous adjunct cultures.

    PubMed

    Piras, Cristina; Cesare Marincola, Flaminia; Savorani, Francesco; Engelsen, Søren B; Cosentino, Sofia; Viale, Silvia; Pisano, Maria Barbara

    2013-12-01

    Fiore Sardo (FS) is a traditional Italian raw ewe's milk cheese carrying a Protected Designation of Origin (PDO). This study investigated the kinetics of FS cheese ripening by physicochemical parameters, microbial counting, and NMR metabolomics using aqueous extracts. Four Fiore Sardo cheeses, manufactured from milk with deliberately added autochthonous lactic acid bacteria (LAB) or commercial starters were studied during a period of 90days of ripening. Major differences in the metabolic profiles were observed amongst the samples as a function of the adjunct culture utilised. (1)H NMR metabolomics in combination with multivariate data analysis was able to classify cheese samples on the basis of their maturation age and the type of added cultures. These findings lay the metabolic basis for the authentication of Fiore Sardo cheese produced in compliance with PDO specifications which allow the use of only native LAB cultures. PMID:23870939

  10. Glucocorticoid receptors in primary cultures of mouse mammary epithelial cells: characterization and modulation by prolactin and cortisol

    SciTech Connect

    Schneider, W.; Shyamala, G.

    1985-06-01

    Mammary epithelial cells isolated from midpregant mice and cultured on collagen gels contain soluble glucocorticoid receptors. The kinetics of binding of dexamethasone reveal a saturable binding site (dissociation constant (K /sub d/), approximately 1 nM), and the binding site obeys a steroid specificity characteristic of a glucocorticoid receptor. As with the receptor isolated from intact glands, the receptor from the cultured cells also requires the addition of dithiothreitol for maximal binding of dexamethasone. The receptors are maintained at in vivo levels (approximately 1.3 pmol/mg DNA) for at least a period of 10 days in culture. However, the presence of both cortisol and PRL is required for the maintenance of the receptors, and the effect of both these hormones is dose dependent.

  11. 3 CFR 9019 - Proclamation 9019 of September 16, 2013. Constitution Day and Citizenship Day, Constitution Week...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...Proclamation 9019 of September 16, 2013. Constitution Day and Citizenship Day, Constitution Week, 2013 9019 Proclamation 9019...of September 16, 2013 Proc. 9019 Constitution Day and Citizenship Day,...

  12. 3 CFR 8714 - Proclamation 8714 of September 16, 2011. Constitution Day and Citizenship Day, Constitution Week...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...Proclamation 8714 of September 16, 2011. Constitution Day and Citizenship Day, Constitution Week, 2011 8714 Proclamation 8714...of September 16, 2011 Proc. 8714 Constitution Day and Citizenship Day,...

  13. 3 CFR 8418 - Proclamation 8418 of September 16, 2009. Constitution Day and Citizenship Day, Constitution Week...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...Proclamation 8418 of September 16, 2009. Constitution Day and Citizenship Day, Constitution Week, 2009 8418 Proclamation 8418...of September 16, 2009 Proc. 8418 Constitution Day and Citizenship Day,...

  14. 3 CFR 8562 - Proclamation 8562 of September 16, 2010. Constitution Day and Citizenship Day, Constitution Week...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...Proclamation 8562 of September 16, 2010. Constitution Day and Citizenship Day, Constitution Week, 2010 8562 Proclamation 8562...of September 16, 2010 Proc. 8562 Constitution Day and Citizenship Day,...

  15. 3 CFR 8862 - Proclamation 8862 of September 13, 2012. Constitution Day and Citizenship Day, Constitution Week...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...Proclamation 8862 of September 13, 2012. Constitution Day and Citizenship Day, Constitution Week, 2012 8862 Proclamation 8862...of September 13, 2012 Proc. 8862 Constitution Day and Citizenship Day,...

  16. Culturing the adolescent brain: what can neuroscience learn from anthropology?

    PubMed Central

    2010-01-01

    Cultural neuroscience is set to flourish in the next few years. As the field develops, it is necessary to reflect on what is meant by ‘culture’ and how this can be translated for the laboratory context. This article uses the example of the adolescent brain to discuss three aspects of culture that may help us to shape and reframe questions, interpretations and applications in cultural neuroscience: cultural contingencies of categories, cultural differences in experience and cultural context of neuroscience research. The last few years have seen a sudden increase in the study of adolescence as a period of both structural and functional plasticity, with new brain-based explanations of teenage behaviour being taken up in education, policy and medicine. However, the concept of adolescence, as an object of behavioural science, took shape relatively recently, not much more than a hundred years ago and was shaped by a number of cultural and historical factors. Moreover, research in anthropology and cross-cultural psychology has shown that the experience of adolescence, as a period of the lifespan, is variable and contingent upon culture. The emerging field of cultural neuroscience has begun to tackle the question of cultural differences in social cognitive processing in adults. In this article, I explore what a cultural neuroscience can mean in the case of adolescence. I consider how to integrate perspectives from social neuroscience and anthropology to conceptualize, and to empirically study, adolescence as a culturally variable phenomenon, which, itself, has been culturally constructed. PMID:19959484

  17. Evaluation of a 3M Petrifilm on-farm culture system for the detection of intramammary infection at the end of lactation.

    PubMed

    Cameron, M; Keefe, G P; Roy, J P; Dohoo, I R; MacDonald, K A; McKenna, S L

    2013-08-01

    The purpose of this study was to evaluate a 3M Petrifilm-based on-farm culture system for the detection of intramammary infection (IMI) in low somatic cell count (SCC) cows (<200,000 cells/mL) at drying off. The main objectives were to determine the test characteristics and the predictive values of the Petrifilm on-farm culture system. The ability of dairy producers to correctly classify cows as infected or uninfected based on Petrifilm culture and a set colony count threshold was also assessed. A total of 360 cows originating from 16 low bulk tank SCC (<250,000 cells/mL) dairy herds were enrolled at drying off. Enrolled cows had an expected dry period of 30-90 days, a SCC<200,000 cells/mL on the last 3 tests prior to drying off, no clinical mastitis in the same time period, and no antibiotic treatment in the last 14 days. Quarter milk samples were collected on the day prior to drying off, and a composite milk sample was created by combining 5 mL of milk from each quarter sample. Composite milk samples were cultured on-farm using the Petrifilm culture system, which provided results within 24h. Quarter milk samples were cultured in a reference laboratory, and the results were aggregated to the cow level. On the day of drying off, the Petrifilm was read by the producer and cows were classified as positive if ?5 colonies (equivalent to 50 colony forming units/mL) were present. When read by the producer, 47.8% of the cows cultured negative on Petrifilm and were infused with only an internal teat sealant at drying off. The test characteristics of the Petrifilm on-farm culture system were calculated by comparing the producer-derived Petrifilm results to those obtained by standard laboratory culture. The sensitivity and specificity of the Petrifilm on-farm culture system were 85.2% (78.5-90.5) and 73.2% (66.4-79.3), respectively. The negative predictive value of the Petrifilm test system was high (86.6%) when estimated using the prevalence of IMI in this data set, and the positive predictive value was moderate (70.9%). An automated 3M Petrifilm reader was used to obtain accurate colony counts. The agreement between Petrifilm results obtained by the producer and those obtained by the automated Petrifilm reader was high, with a kappa value of 0.82 (0.75-0.89). PMID:23597619

  18. Cytochrome P450 mono-oxygenase gene expression and protein activity in cultures of adult cardiomyocytes of the rat

    PubMed Central

    Thum, Thomas; Borlak, Jürgen

    2000-01-01

    There are a substantial number of drugs acting either directly or indirectly on the heart, but surprisingly, little is known about the metabolic capacity of heart muscle cells.We therefore investigated the gene expression and protein activity of cytochrome P450 isozymes in cultures of adult cardiomyocytes of the rat.Semi-quantitative CYP gene expression pattern suggests CYP1A1 and CYP2B1/2 to be key players in cardiomyocytes and upon treatment with Aroclor 1254 approximate 4 fold inductions could be observed for both gene families, when compared with appropriate controls.The mRNA expression of most genes was sustained for prolonged periods of time, e.g. up to 120?h in culture and in the case of the CYP3A1 gene an approximate 10 fold induction was observed at the higher Aroclor 1254 dose level (10??M) in 24?h old cultures.The constitutively expressed genes, e.g. CYP2C11 and CYP2E1 are expressed throughout the entire culture period (5 days) and did not respond to Aroclor 1254 treatment.CYP4A1 was mainly expressed in freshly isolated cardiomyocytes of control animals and its expression declined rapidly in culture.There was good agreement between gene expression and translated protein activity using 7-ethoxyresorufin and testosterone as substrates.The data reported herein should foster the routine use of freshly isolated and cultivated cardiomyocytes for drug profiling and toxicity studies. PMID:10952662

  19. Spaceflight effects on cultured embryonic chick bone cells

    NASA Technical Reports Server (NTRS)

    Landis, W. J.; Hodgens, K. J.; Block, D.; Toma, C. D.; Gerstenfeld, L. C.

    2000-01-01

    A model calcifying system of primary osteoblast cell cultures derived from normal embryonic chicken calvaria has been flown aboard the shuttle, Endeavour, during the National Aeronautics and Space Administration (NASA) mission STS-59 (April 9-20, 1994) to characterize unloading and other spaceflight effects on the bone cells. Aliquots of cells (approximately 7 x 10(6)) grown in Dulbecco's modified Eagle's medium (DMEM) + 10% fetal bovine serum (FBS) were mixed with microcarrier beads, inoculated into cartridge culture units of artificial hollow fiber capillaries, and carried on the shuttle. To promote cell differentiation, cartridge media were supplemented with 12.5 microg/ml ascorbate and 10 mM beta-glycerophosphate for varying time periods before and during flight. Four cartridges contained cells from 17-day-old embryos grown for 5 days in the presence of ascorbate prior to launch (defined as flight cells committed to the osteoblastic lineage) and four cartridges supported cells from 14-day-old embryos grown for 10 days with ascorbate before launch (uncommitted flight cells). Eight cartridges prepared in the same manner were maintained under normal gravity throughout the flight (control cells) and four additional identical cartridges under normal gravity were terminated on the day of launch (basal cells). From shuttle launch to landing, all cartridges were contained in closed hardware units maintaining 5% CO2, 37 degrees C, and media delivery at a rate of approximately 1.5 ml/6 h. During day 3 and day 5 of flight, duplicate aliquots of conditioned media and accumulated cell products were collected in both the flight and the control hardware units. At the mission end, comparisons among flight, basal, and control samples were made in cell metabolism, gene expression for type I collagen and osteocalcin, and ultrastructure. Both committed and uncommitted flight cells were metabolically active, as measured by glucose uptake and lactate production, at approximately the same statistical levels as control counterparts. Flight cells elaborated a less extensive extracellular matrix, evidenced by a reduced collagen gene expression and collagen protein appearance compared with controls. Osteocalcin was expressed by all cells, a result indicating progressive differentiation of both flight and control osteoblasts, but its message levels also were reduced in flight cells compared with ground samples. This finding suggested that osteoblasts subjected to flight followed a slower progression toward a differentiated function. The summary of data indicates that spaceflight, including microgravity exposure, demonstrably affects bone cells by down-regulating type I collagen and osteocalcin gene expression and thereby inhibiting expression of the osteogenic phenotype notably by committed osteoblasts. The information is important for insight into the response of bone cells to changes of gravity and of force in general.

  20. Kinetics of glucosylated and non-glucosylated aryltetralin lignans in Linum hairy root cultures.

    PubMed

    Huynh Cong, Luyen; Dauwe, Rebecca; Lequart, Michelle; Vinchon, Sophie; Renouard, Sullivan; Fliniaux, Ophélie; Colas, Cyril; Corbin, Cyrielle; Doussot, Joël; Hano, Christophe; Lamblin, Frédéric; Molinié, Roland; Pilard, Serge; Jullian, Nathalie; Boitel, Michèle; Gontier, Eric; Mesnard, François; Laberche, Jean-Claude

    2015-07-01

    Due to their pronounced cytotoxic activity, a number of aryltetralin lignans (ATLs), such as podophyllotoxin (PTOX), are used as antitumor compounds. The production of such molecules from entire plants or plant cell-tissue-organ cultures is thus of interest to the pharmaceutical industry. Hairy root cultures constitute a good tool not only for phytochemical production but also for investigating plant secondary metabolism. This work reports on the growth and ATL biosynthesis in two hairy root cultures of Linum album Kotschy ex Boiss. and Linum flavum. The kinetics of accumulation of the intermediates of MPTOX biosynthesis and of their glucosylated forms are described over a 21-day period of growth. An accumulation of non-glucosylated forms of the ATLs during the exponential phase of the cultures is followed by an accumulation of the glucosylated forms during the stationary phase. Our results show a strong coordination of the biosynthetic paths derived from deoxypodophyllotoxin via deoxypodophyllotoxin 6-hydroxylase and deoxypodophyllotoxin 7-hydroxylase, and a coordinated glucosylation of podophyllotoxin, methoxypodophyllotoxin, and 5'-demethoxymethoxypodophyllotoxin. Furthermore, our results suggest an important role of ?-peltatin-6-glucoside formation in the control of ATL accumulation in Linum hairy root cultures. PMID:25698360

  1. 40- to 60-day oscillation in the source region of the Somali Current during 1976

    Microsoft Academic Search

    Lawrence A. Mysak; Gordon J. Mertz

    1984-01-01

    Evidence is presented for a 40- and 60-day oscillation in the longshore current and temperature fluctuations that were measured during 1976 in the Somali Current regime off Kenya by Dueing and Schott (1978). It is suggested that this equatorial oceanic oscillations is excited by long-period local wind fluctuations which are associated with the global tropical 40- to 50-day day oscillation

  2. 77 FR 62437 - Regulated Navigation Area; Columbus Day Weekend, Biscayne Bay, Miami, FL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-15

    ...1625-AA11 Regulated Navigation Area; Columbus Day Weekend, Biscayne Bay, Miami, FL AGENCY...The Coast Guard is amending the Columbus Day weekend regulated navigation area on Biscayne...necessary to protect the public during Columbus Day weekend; a period that has...

  3. In vitro culture, storage and transfer of goat embryos.

    PubMed

    Bilton, R J; Moore, N W

    1976-03-01

    Goat embryos collected 5 and 7 days after mating, were cultured in vitro at 37 degrees C for 2 days, or stored at 5 degrees C for 1 or 2 days and then cultured for 2 days, or stored in liquid nitrogen (-196 degrees C) for 2-4 weeks and then cultured for 1 day. After culture some of the embryos were transferred to recipient does. Culture and storage was carried out in Dulbecco phosphate buffer enriched with 25% goat serum. 1M glycerol or 2M dimethylsulphoxide (DMSO) was added to the media used for frozen storage. Thirteen of 15 embryos cultured without prior storage showed apparently normal development in culture. Ten of the 13 were transferred and five kids were born. Twenty of 38 embryos stored at 5 degrees C developed in culture and six kids were born following the transfer of 17 embryos. Duration of storage at 5 degrees C had no marked effect upon subsequent development. Six of 48 frozen stored embryos developed in culture. All six were transferred and three kids were born. PMID:962719

  4. International Literacy Day Tool Kit.

    ERIC Educational Resources Information Center

    2002

    This tool kit suggests various International Literacy Day activities to raise awareness of the issues of adult literacy and language learning, to connect local literacy programs with national programs, and to help achieve the National Literacy Summit goal by 2010. The kit is intended for individuals, programs, and organizations that want to call…

  5. The Twelve Days of Christmas

    NSDL National Science Digital Library

    Brown, Judy Ann

    Add song and dance to your mathematics lessons with this exercise, in which students discover the various covert mathematical relations hidden in the lyrics to the song â??The Twelve Days of Christmas.â? In addition to the specific song-related questions, the activity provides guidance for instructors to create similar projects with other songs.

  6. A Day in the Life...

    ERIC Educational Resources Information Center

    Ansenberg, Dania; Branch, Jennifer L.; Silvennoinen, Anneli; Wilson, Kay; McClurg, Kati; Baffour-Awuah, Margaret; Clyde, Anne; Free, John; Oberg, Dianne

    2000-01-01

    These nine articles present narrative accounts of typical days in the working life of school librarians from all over the world. Includes school librarians, teacher-librarians, network librarians, Peace Corps volunteers, and Webmasters, as well as a report from the IASL (International Association of School Librarianship) Web site. (LRW)

  7. Take Advantage of Constitution Day

    ERIC Educational Resources Information Center

    McCune, Bonnie F.

    2008-01-01

    The announcement of the mandate for Constitution and Citizenship Day shortly before September, 2005, probably led to groans of dismay. Not another "must-do" for teachers and schools already stressed by federal and state requirements for standardized tests, increasingly rigid curricula, and scrutiny from the public and officials. But the idea and…

  8. Infectious Diseases in Day Care.

    ERIC Educational Resources Information Center

    Sleator, Esther K.

    Discussed in this publication are infectious illnesses for which children attending day care appear to be at special risk. Also covered are the common cold, some infectious disease problems receiving media attention, and some other annoying but not serious diseases, such as head lice, pinworms, and contagious skin conditions. Causes,…

  9. Experiments for a Special Day

    ERIC Educational Resources Information Center

    Gluck, Paul

    2008-01-01

    Special events like science days, teacher's meetings and physics recruiting efforts require spectacular and, if possible, interactive experiments for the audience. Based on past experience with such events, we have gathered and present here a series of demonstration experiments in mechanics, optics, waves and electricity which are suitable, and…

  10. A New Day for Kids

    ERIC Educational Resources Information Center

    Farbman, David

    2007-01-01

    The Martin Luther King School in Boston and nine other Massachusetts public schools used a grant from the Massachusetts Department of Education to expand their school days by at least two hours. Each school lengthened the time students spent in reading and math instruction. Farbman focuses on the Martin Luther King School's foray into an extended…

  11. United Nations Day, 24 October.

    ERIC Educational Resources Information Center

    Osborne, Ken, Ed.

    1993-01-01

    Serving as the journal of the Manitoba Social Science Teachers' Association, this issue commemorates United Nations Day with the editorial, "Teaching about the United Nations" (Ken Osborne). Another article devoted to the international organization is "The United Nations and International Peace and Security" (Ken Osborne). The article is intended…

  12. Music in Our Schools Day

    ERIC Educational Resources Information Center

    Music Educators Journal, 1973

    1973-01-01

    Article focused attention on the first national Music in Our Schools Day, whose purpose is to stress educational goals and processes of music education in the schools so that community understanding of and support for music education will be increased. (Author/RK)

  13. Day Care Management. Final Report.

    ERIC Educational Resources Information Center

    Bourque, Janet

    A curriculum was developed and a pilot project was conducted to train 20 day care center directors at Lake Washington Vocational Technical Institute. This document summarizes the curriculum development project and provides the curriculum that was developed. The report contains a summary and outline of the course, a skills assessment, pretests and…

  14. Handbook for Day Care Professionals.

    ERIC Educational Resources Information Center

    Day Care Information Service, Bethesda, MD.

    This handbook for day care professionals is organized in 13 sections. Topics discussed are adminstration, advocacy, computerization, employer-sponsored child care, funding and fundraising, information and referral, legal issues, licensing and regulatory issues, school age child care, state initiatives, training, and health and safety. A special…

  15. [Flower initiation by cycloheximide in the long-day plant Hyoscyamus niger during short-days].

    PubMed

    Klautke, S; Rau, W

    1973-03-01

    Cycloheximide (CH) was applied selectively either to the shoot apex or by infiltration to the leaves of the long-day plant Hyoscyamus niger in order to investigate whether this inhibitor has an effect on the synthesis of a floral stimulus in the leaves. Treatment of the shoot apex with CH caused inhibition of the photoperiodic induction. In contrast, when CH was applied to leaves, initiation of flowering was observed under short-day conditions. The drug yielded optimum initiating effects at concentrations of 10(-5)-3·10(-5) M, inducing flowering of almost 60% of the plants. Daily infiltration over a period of up to 4 days decreased the rate of flower initiation. The effect of CH was shown to be additive to a photoperiodic induction, even to a sub-threshold induction, but not to 2-thiouracil mediated induction. In no case did the presence of additional untreated leaves on the plants suppress CH-mediated flower induction. Treatment of the leaves with chloramphenicol (10(-6)-2(-)10(-4) M) or puromycin (5·10(-6)-2·10(-4) M) caused no initiating response. The results are interpreted to mean that the presence of CH in the leaves may lead to the synthesis of a floral stimulus also under short-day conditions. This finding is similar to that reported previously in the case of the inductive effect of 2-thiouracil. PMID:24469780

  16. How culture impacts projects

    Microsoft Academic Search

    T Kippenberger

    2000-01-01

    Projects that even the most battle-hardened project manager will acknowledge that manager’s mental models, their attitudes, the competences they rate, etc., all have major implications. Looks at in-house cultures, external cultures, dysfunctional cultures and international cultures. Uses 2 Figures to emphasize both the iceberg model of inter-cultural business analysis and cultural dimensions. Weighs up some of the differences between cultures

  17. Secure Bike Storage Rental Application Rental Periods

    E-print Network

    Thompson, Michael

    is rentable based on the academic term schedule. All rental periods expire on the last day of the arranged be purchased at the cost of $5.00. Any cards reported lost or stolen will be deactivated. Should you notice any suspicious activity around the Secure Bike Storage facility, you can reach Security Services 24/7 by calling

  18. 3382 Cassidy: A Short Period Asteroid

    NASA Astrophysics Data System (ADS)

    Risley, Ethan

    2013-04-01

    The asteroid 3382 Cassidy was observed from the Etscorn Campus Observatory (ECO, 2012) at New Mexico Institute of Mining and Technology in Socorro, NM, on nine nights over a span of 43 days in 2012 September-November. A bimodal synodic period of 4.254 ± 0.002 h and an amplitude of 0.15 ± 0.02 mag were obtained.

  19. Day-to-day variations of temperature in Texas 

    E-print Network

    Breese, Richard Preston

    1968-01-01

    OF SCIENCE May ~ Ha)or $$)ect: METEOROLOGY DAY-TG-'1~Y 7APT". . TG!JS GF TE a. '~TLiPZ TB TrZ iS A Thesis RICrULFZ PPZSTCJ PZ~?; Captain Urd ted S". a"';=s AU' Foroo Approved as to stg1e and content by: TIGXl of oiiL". 0 John ~ Grif fiths Robert A... Temperature ~ ~ 3p viii LIS OF TABLE TABLE 1 The Average I. V ~ at Specif'ic Hours of the Day Page a ~ ~ 5 2 The I'~. ~~m I. 7 ~ of Tenperature and the Hour of Occurrence 6 3 The I. 7. of the I'iean Temperature ard the Degree of Contincntality...

  20. Five-Day Cefdinir Treatment for Streptococcal Pharyngitis

    PubMed Central

    Tack, Kenneth J.; Henry, Daniel C.; Gooch, W. Manford; Brink, Douglas N.; Keyserling, Constance H.; Group, The Cefdinir Pharyngitis Study

    1998-01-01

    A multicenter, randomized, controlled, investigator-blind study was performed to evaluate the safety and efficacy of oral cefdinir versus oral penicillin V for the treatment of pharyngitis due to group A beta-hemolytic streptococci (GABHS). Patients 13 years of age and older were randomized to receive either oral cefdinir (300 mg twice a day) for 5 days followed by placebo for 5 days or oral penicillin V (250 mg four times a day) for 10 days. Throat cultures were obtained, and signs and symptoms of pharyngitis were recorded at study admission and follow-up visits on study days 11 to 15, 16 to 20, and 25 to 31. Patients kept a diary to record medication intake and their assessment of throat pain at admission and at each day of study treatment. Five hundred fifty-eight patients were enrolled, of whom 432 (77.4%) were clinically and microbiologically evaluable. The GABHS eradication rates 5 to 10 days after completion of therapy were 193 of 218 (88.5%) in the cefdinir group and 176 of 214 (82.2%) in the penicillin group (P = 0.053). Clinical cure rates were 89.0 and 84.6%, respectively (P = 0.80). By the time of the long-term follow-up visit, 2 to 3 weeks after completion of treatment, 156 of 191 (81.7%) of the assessable cefdinir patients and 152 of 195 (77.9%) of the penicillin patients remained free of GABHS. Both treatments were well tolerated, with adverse reaction rates of 18.3% in the cefdinir study arm and 15.0% in the penicillin study arm (P = 0.278). Five-day treatment with cefdinir is safe and effective therapy for GABHS pharyngitis. Based on its twice-a-day dosage and shorter course of therapy, leading to potentially greater patient compliance, cefdinir may be considered for use in the treatment of pharyngitis caused by GABHS. PMID:9593129

  1. What I'm Really Looking For: Family Day Care from the Parent's Perspective

    ERIC Educational Resources Information Center

    Huang, Grace Hui-Chen

    2007-01-01

    The purpose of this study was to describe parents' experiences in sending their children to non-regulated family day care. Noticeably absent were studies about non-regulated family day care culture as well as parental experiences. Phenomenology, the study of human experiences, was the methodology used to identify the meaning behind parents' search…

  2. 24 CFR 203.266 - Period covered by periodic MIP.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...2011-04-01 false Period covered by periodic MIP. 203.266 Section 203.266 ...Obligations Mortgage Insurance Premiums-Periodic Payment § 203.266 Period covered by periodic MIP. The initial MIP shall...

  3. 24 CFR 203.266 - Period covered by periodic MIP.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...2012-04-01 false Period covered by periodic MIP. 203.266 Section 203.266 ...Obligations Mortgage Insurance Premiums-Periodic Payment § 203.266 Period covered by periodic MIP. The initial MIP shall...

  4. 24 CFR 203.266 - Period covered by periodic MIP.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...2010-04-01 false Period covered by periodic MIP. 203.266 Section 203.266 ...Obligations Mortgage Insurance Premiums-Periodic Payment § 203.266 Period covered by periodic MIP. The initial MIP shall...

  5. 24 CFR 203.266 - Period covered by periodic MIP.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...2013-04-01 false Period covered by periodic MIP. 203.266 Section 203.266 ...Obligations Mortgage Insurance Premiums-Periodic Payment § 203.266 Period covered by periodic MIP. The initial MIP shall...

  6. 24 CFR 203.266 - Period covered by periodic MIP.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...2014-04-01 false Period covered by periodic MIP. 203.266 Section 203.266 ...Obligations Mortgage Insurance Premiums-Periodic Payment § 203.266 Period covered by periodic MIP. The initial MIP shall...

  7. Improving School Climate: Alternating-Day Block Schedule.

    ERIC Educational Resources Information Center

    Hackmann, Donald G.

    1995-01-01

    Examines the implementation of an alternating day schedule in middle school. It is suggested that block scheduling provides an effective instructional alternative to the traditional six- or seven-period format, creating a relaxed atmosphere while decreasing stress and improving attitudes of both students and teachers. Blocks provide a gradual…

  8. Holter monitoring: are two days better than one?

    NASA Technical Reports Server (NTRS)

    McClennen, S.; Zimetbaum, P. J.; Ho, K. K.; Goldberger, A. L.

    2000-01-01

    The optimal duration of Holter monitoring (HM) to minimize costs and maximize yield is unknown. In a retrospective review of 164 patients referred to a tertiary care center for evaluation with 2 days of HM, we found that 48 hours was not cost effective when compared with the traditional 24-hour period.

  9. A Day in the Life of a Student Teacher.

    ERIC Educational Resources Information Center

    Reiff, Rebecca

    This paper follows a student teacher through a typical day in her high school student teaching placement, beginning with her pre-dawn departure for school, and moving from her preparations for class to her classes, which begin at 7:15 a.m. (homeroom, several periods of biology, lunch, planning, more biology, and biochemistry). She focuses on her…

  10. Redesigning the School Day: A User-Friendly Schedule.

    ERIC Educational Resources Information Center

    Rettig, Michael D.; Colbert, Chris K.

    1995-01-01

    The implementation of block scheduling by a middle school for deaf and blind students is described. Longer class periods on alternating days were found to expand teachers' opportunities to plan interdisciplinary instruction that addressed individual learning styles. An extension center was developed to provide reinforcement, enrichment, and…

  11. Increasing fall fruit production in short-day strawberries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the mid-Atlantic coast region of the United States (30 deg N, 77 deg W), the harvest period for short-day (SD) strawberry cultivars is only 7 weeks long from early May to late June. Strawberry transplants from a new propagation technique that improves fall flowering were grown under high tunnel ...

  12. Open Day 2011/2012 School of Physical Sciences

    E-print Network

    Humphrys, Mark

    traditional manufacturing processes use materials because of their intrinsic properties. Why does one element in the periodic table underpin the technological developments in the information age? Impact of physics on every-day, Optics, Thermal Physics, Electricity and Magnetism, Chemistry, Computing, Labs and Projects Year 2

  13. 7 day chronic ceriodaphnia toxicity test -- reproductive

    SciTech Connect

    Not Available

    1989-01-01

    This toxicity test was conducted to determine if the effluent causes death (acute toxicity) or reduction in the reproduction of the test organisms (chronic toxicity) during a seven day period. A series of dilutions of the effluent are set to determine how much the effluent must be diluted before toxic effects are no longer noted. Acute toxicity is checked by statistically analyzing whether significantly more organisms die in the effluent dilutions than in the control treatment, and, if significantly more die, how much the effluent must be diluted so as to kill only 50% of the test organisms (the LC50). Chronic toxicity is checked by statistically analyzing whether significantly fewer young are produced by test organisms exposed to the effluent dilutions. Results indicate the lowest effluent concentration which shows a toxic effect (the LOEC) and the highest effluent concentration which does not demonstrate an effect (NOEC).

  14. This World: One Day of War

    NSDL National Science Digital Library

    The investigative news program titled This World is one of the BBC's many programs dedicated to providing in-depth coverage on a number of pressing issues around the world. Their recent project, One Day of War, follows individual fighters in 16 of the wars currently going on around the world over the same 24-hour period. The centerpiece of the site is the section that offers profiles of these 16 people, along with a complete transcript of the original program that aired at the end of May 2004. Some of the people profiled here include Shushila Magar, who is fighting with the Nepali Maoist Militia, and Nati Mazuz, who is a professional solider serving in the Gaza Strip. Also available is a commentary from the series producer on how the project came to be, and a clickable map where individuals can learn more about each region featured in the personal stories of each participant.

  15. A tunable Caco-2/HT29-MTX co-culture model mimicking variable permeabilities of the human intestine obtained by an original seeding procedure.

    PubMed

    Béduneau, Arnaud; Tempesta, Camille; Fimbel, Stéphane; Pellequer, Yann; Jannin, Vincent; Demarne, Frédéric; Lamprecht, Alf

    2014-07-01

    Standard monoculture models utilizing Caco-2 monolayers were extensively used to mimic the permeability of the human intestinal barrier. However, they exhibit numerous limitations such as the lack of mucus layer, an overestimation of the P-gp-mediated efflux and a low paracellular permeability. Here, we suggest a new procedure to set up an in vitro model of intestinal barrier to adjust gradually the properties of the absorption barrier. Mucin-secreting HT29-MTX cells were added to Caco-2 absorptive cells in a Transwell® at different time intervals. Effects of seeding day of HT29-MTX on the paracellular permeability of lucifer yellow (LY) and on the P-gp-mediated efflux of rhodamine 123 were investigated. Apparent permeability of the rhodamine 123 in the secretory direction was highly dependent on the seeding day of goblet cells. Transepithelial electrical resistance values and LY transport across the co-cultures in the apical-to-basolateral direction were intermediary between single Caco-2 and HT29-MTX models. Early seeding days of HT29-MTX allowed increasing the fraction of goblet cells in the co-culture. Co-culture permeability was unchanged between 21 and 30 days after Caco-2 seeding, corresponding to the period of use for Caco-2-based cell models. Thus, the HT29-MTX seeding day was a key factor to set up an in vitro intestinal model with tailor-made barrier properties in terms of P-gp expression and paracellular permeability. PMID:24704198

  16. Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test

    Microsoft Academic Search

    Masahide Tanaka; Nobuaki Nakakita; Yoshimitsu Kuroyanagi

    1999-01-01

    The authors developed a cultured dermal substitute (CDS) composed of a spongy collagen containing cultured fibroblasts. The cultured fibroblasts derived from Sprague Dawley rat skin were seeded on a spongy collagen at a density of 5 x 10 cells cm and cultured for 7 days. This CDS was applied to the debrided wound of full-thickness burn which was inflicted experimentally

  17. Periodic chiral structures

    NASA Technical Reports Server (NTRS)

    Jaggard, Dwight L.; Engheta, Nader; Pelet, Philippe; Liu, John C.; Kowarz, Marek W.; Kim, Yunjin

    1989-01-01

    The electromagnetic properties of a structure that is both chiral and periodic are investigated using coupled-mode equations. The periodicity is described by a sinusoidal perturbation of the permittivity, permeability, and chiral admittance. The coupled-mode equations are derived from physical considerations and used to examine bandgap structure and reflected and transmitted fields. Chirality is observed predominantly in transmission, whereas periodicity is present in both reflection and transmission.

  18. Culture and web communications

    Microsoft Academic Search

    Daniel W. Baack; Nitish Singh

    2007-01-01

    Various studies find that marketing communications reflect specific cultures. Most of these studies conceptualize culture in terms of one cultural framework, use a two-country sample, focus on print or television advertising, and do not statistically validate the measures used. This study addresses these issues by testing the applicability of the cultural frameworks of Hofstede [Hofstede G. Culture's consequences: international differences

  19. Proportion of inpatient days for mental disorders: 1969-1978.

    PubMed

    Kiesler, C A; Sibulkin, A E

    1983-07-01

    Mental hospitalization is an important part of the national picture of hospitalization. Total inpatient days for mental disorders, and their proportion of total hospital days for all disorders, is a statistic of considerable national import, yet there has never been a complete description of total inpatient days for mental disorders. The authors present data from the National Center for Health Statistics for nine years (data for only two years had been published previously), and correct them, and national totals, for the previous exclusion of data from residential treatment centers and community mental health centers. From 1969 through 1978, inpatient days for mental disorders fell from 168 million to 95 million. This decrease was more than accounted for by decreased psychiatric inpatient days at two sites: state mental hospitals and Veterans Administration psychiatric hospitals. The number of psychiatric inpatient days at all other sites increased during this time period. The authors also note a decrease in total inpatient days for all disorders over the decade. However, 85 percent of the total national decrease was accounted for by the decrease in psychiatric inpatient days. The authors stress the implications for public policy and the need for a more adequate national data base. PMID:6885014

  20. 75 FR 57835 - Constitution Day and Citizenship Day, Constitution Week, 2010

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-22

    ...Day and Citizenship Day, Constitution Week, 2010 Proclamation 8563--National POW...Day and Citizenship Day, Constitution Week, 2010 By the President of the United States...Citizenship Day, and during Constitution Week, we commemorate the legacy passed...