Science.gov

Sample records for day culture period

  1. Aim for 55-Day Dry Period for Top Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The research cited in this article determined the optimal dry period length (the number of days between lactations when a cow is not being milked) for US Jersey cows using traits such as milk, fat, and protein yield, fat and protein percentages, somatic cell score, and days open. This was accomplis...

  2. Cultural Support Workers and Long Day Care Services

    ERIC Educational Resources Information Center

    Miller, Melinda G.; Knowles, Meg; Grieshaber, Susan

    2011-01-01

    In Australia, eligible long day care services may apply for support at the state level to assist with the transition of children from culturally or linguistically diverse backgrounds into childcare settings. For staff in childcare services, this support comes in the form of a cultural support worker (CSW). The primary role of a CSW is to build…

  3. 12 CFR 329.104 - Ten-day grace period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY... periods during which interest may be paid on a deposit without violating § 329.2. (a) During the ten calendar days following the maturity of a time deposit, the bank may continue to pay interest on...

  4. Performance of stool cultures before and after a 3-day hospitalization: fewer cultures, better for patients and for money.

    PubMed

    Le Guern, Rémi; Loïez, Caroline; Grandbastien, Bruno; Courcol, René; Wallet, Frédéric

    2013-09-01

    We evaluated retrospectively the yield of stool culture (SC) depending on the length of hospitalization, and we characterized the patients missed by the 3-day rejection rule. SC detects bacterial enteric pathogens (Campylobacter spp., Salmonella enterica, Yersinia spp., Shigella spp.). During this 5-year study period, 13,039 SCs were requested, and 376 were positive (2.9%). The yield of SC dropped from 11.7% before 3 days of hospitalization to 0.7% after 3 days in children and 4.3% to 0.3% in adults. Finally, only 13 clinically relevant cases (0.2% of SC prescribed after 3 days) were undiagnosed by strict application of the 3-day rule. In conclusion, rejection of SC prescribed after 3 days of hospitalization allows to reduce workload by 37.8% for children and 65.7% for adults, representing a cost of €12,500 ($16,250) per year in our hospital. PMID:23867328

  5. 77 FR 64759 - Rescission of 10-Day Agency Discretionary Period in Assigning Unsatisfactory Safety Ratings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-23

    ...FMCSA-2012-0262] RIN 2126-AB55 Rescission of 10-Day Agency Discretionary Period in Assigning Unsatisfactory...provision indicating that the Agency will consider a 10- day extension of the 45-day period after which passenger and hazardous...

  6. Automating Partial Period Bond Valuation with Excel's Day Counting Functions

    ERIC Educational Resources Information Center

    Vicknair, David; Spruell, James

    2009-01-01

    An Excel model for calculating the actual price of bonds under a 30 day/month, 360 day/year day counting assumption by nesting the DAYS360 function within the PV function is developed. When programmed into an Excel spreadsheet, the model can accommodate annual and semiannual payment bonds sold on or between interest dates using six fundamental…

  7. Periodic CO2 Dosing Strategy for Dunaliella salina Batch Culture

    PubMed Central

    Ying, Kezhen; Gilmour, D. James; Zimmerman, William B.

    2015-01-01

    A periodic CO2 dosing strategy for D. salina 19/30 batch culture is proposed. A model of periodic CO2 dosing including dosing time calculation, dosing interval estimation and final chlorophyll yield prediction was established. In experiments, 5% CO2/95% N2 gas was periodically dosed into D. salina culture. Two different gas dosing flow rates were tested. The corresponding dosing time for each flow rate was estimated via the model (10 min·d?1 for 0.7 L·min?1 and 36 min·d?1 for 0.3 L·min?1). Daily pH measurements showed that the pH of these cultures dosed periodically was always kept between 7.5 and 9.5, which highlights that periodic gas supply can maintain a suitable range of pH for microalgal growth without expensive buffers. Notably the culture dosed for set daily intervals was seen to have similar growth to the culture supplied constantly, but with much higher CO2 capture efficiency (11%–18%) compared to continuous dosing (0.25%). It shows great potential for using periodic gas supply to reduce cost, wasted gas and energy use. PMID:25997005

  8. Artery Remodeling Under Axial Twist in Three Days Organ Culture.

    PubMed

    Wang, Guo-Liang; Xiao, Yangming; Voorhees, Andrew; Qi, Ying-Xin; Jiang, Zong-Lai; Han, Hai-Chao

    2015-08-01

    Arteries often endure axial twist due to body movement and surgical procedures, but how arteries remodel under axial twist remains unclear. The objective of this study was to investigate early stage arterial wall remodeling under axial twist. Porcine carotid arteries were twisted axially and maintained for three days in ex vivo organ culture systems while the pressure and flow remained the same as untwisted controls. Cell proliferation, internal elastic lamina (IEL) fenestrae shape and size, endothelial cell (EC) morphology and orientation, as well as the expression of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and tissue inhibitor of metalloproteinase-2 (TIMP-2) were quantified using immunohistochemistry staining and immunoblotting. Our results demonstrated that cell proliferation in both the intima and media were significantly higher in the twisted arteries compared to the controls. The cell proliferation in the intima increased from 1.33 ± 0.21% to 7.63 ± 1.89%, and in the media from 1.93 ± 0.84% to 8.27 ± 2.92% (p < 0.05). IEL fenestrae total area decreased from 26.07 ± 2.13% to 14.74 ± 0.61% and average size decreased from 169.03 ± 18.85 ?m(2) to 80.14 ± 1.96 ?m(2) (p < 0.01), but aspect ratio increased in the twist group from 2.39 ± 0.15 to 2.83 ± 0.29 (p < 0.05). MMP-2 expression significantly increased (p < 0.05) while MMP-9 and TIMP-2 showed no significant difference in the twist group. The ECs in the twisted arteries were significantly elongated compared to the controls after three days. The angle between the major axis of the ECs and blood flow direction under twist was 7.46 ± 2.44 degrees after 3 days organ culture, a decrease from the initial 15.58 ± 1.29 degrees. These results demonstrate that axial twist can stimulate artery remodeling. These findings complement our understanding of arterial wall remodeling under mechanical stress resulting from pressure and flow variations. PMID:25503524

  9. Atmospheric angular momentum and the length of day - A common fluctuation with a period near 50 days

    NASA Technical Reports Server (NTRS)

    Langley, R. B.; King, R. W.; Shapiro, I. I.; Rosen, R. D.; Salstein, D. A.

    1981-01-01

    Four astronomical measures of changes in the length of day obtained in 1979 have been shown to exhibit the same, approximately 50-day fluctuation. To find whether this fluctuation was persistent, and of meteorological origin, lunar laser ranging observations and wind data deduced from sources distributed over the globe were analyzed. A high degree of correlation was found between the two sets of data. It is implied that the 50-day period fluctuations in length of day are real and related to meteorological effects. Observed changes in length of day can provide a constraint for models for atmospheric flow, and a partial check for global analyses of such motions.

  10. 77 FR 42790 - Culturally Significant Objects Imported for Exhibition Determinations: “The Last Days of Pompeii...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Culturally Significant Objects Imported for Exhibition Determinations: ``The Last Days of Pompeii: Decadence... ``The Last Days of Pompeii: Decadence, Apocalypse, Resurrection,'' imported from abroad for...

  11. 29 CFR 553.230 - Maximum hours standards for work periods of 7 to 28 days-section 7(k).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...Maximum hours standards for work periods of 7 to 28 days...employees engaged in fire protection activities who have a work period of at least...nearest whole hour): Work period (days) Maximum hours standards Fire protection Law...

  12. 29 CFR 553.230 - Maximum hours standards for work periods of 7 to 28 days-section 7(k).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...Maximum hours standards for work periods of 7 to 28 days...employees engaged in fire protection activities who have a work period of at least...nearest whole hour): Work period (days) Maximum hours standards Fire protection Law...

  13. 29 CFR 553.230 - Maximum hours standards for work periods of 7 to 28 days-section 7(k).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...Maximum hours standards for work periods of 7 to 28 days...employees engaged in fire protection activities who have a work period of at least...nearest whole hour): Work period (days) Maximum hours standards Fire protection Law...

  14. 29 CFR 553.230 - Maximum hours standards for work periods of 7 to 28 days-section 7(k).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...Maximum hours standards for work periods of 7 to 28 days...employees engaged in fire protection activities who have a work period of at least...nearest whole hour): Work period (days) Maximum hours standards Fire protection Law...

  15. 29 CFR 553.230 - Maximum hours standards for work periods of 7 to 28 days-section 7(k).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...Maximum hours standards for work periods of 7 to 28 days...employees engaged in fire protection activities who have a work period of at least...nearest whole hour): Work period (days) Maximum hours standards Fire protection Law...

  16. Quasi-16-day periodic meridional movement of the equatorial ionization anomaly

    E-print Network

    Mo, X. H.

    Based on the daytime location of the equatorial ionization anomaly (EIA) crest derived from GPS observations at low latitude over China during the 2005–2006 stratospheric sudden warming (SSW), a quasi-16-day periodic ...

  17. Long-Period Cultural Noise: The Panama Canal Seiche

    NASA Astrophysics Data System (ADS)

    McNamara, D. E.; Hutt, C. R.; Ringler, A.; Gee, L. S.

    2009-12-01

    Traditionally, the spectral composition of human generated or “cultural” seismic noise is dominated by short-period (SP) energy (<1s). In this study, we present evidence for long-period (LP) cultural noise (100-200s) at the USGS Global Seismographic Network (GSN) station CU.BCIP, located at the Smithsonian Tropical Research Institute (STRI) on Barro Colorado Island (BCI) in the Panama Canal. The LP seismic energy has a strong diurnal signal, with highest power during the daytime when traffic through the Panama Canal is the heaviest, ~1 ship every 10 minutes. The lowest power occurs during the late-night/early-morning hours, when container ship traffic slows to ~1 ship every hour. Spectral power observations are corroborated with data recorded by a water-level meter located approximately 80m from the seismic station, indicating that water waves are the primary source of the LP seismic signal. We show that the observed water waves are a “seiche” induced by the wakes of container ship traffic in the canal. As passing ships disturb the water surface, standing waves are induced by the summation of propagating waves, traveling in opposite directions, due to reflections off the opposite shorelines of the Panama Canal. Vertical harmonic motion results as gravity seeks to restore the horizontal surface of the body of water to a state of hydrostatic equilibrium. The longest natural-period of a seiche in an enclosed body of water is a function of basin depth and length and can be computed by a common oceanographic relationship, known as the Merian formula. For a reasonable range of Panama Canal dimensions in the vicinity of BCI, (depths from 15 to 30m and lengths from 700 to 1500m), the predicted dominant period of a seiche is between 100-200s, consistent with our seismic spectral power observations. Small rhythmic seiches are always present in disturbed enclosed bodies of water and are most often caused by either meteorological effects (wind and atmospheric pressure variations), and/or seismic activity (earthquakes, tsunamis). Historical accounts of anomalously large seiches in enclosed bodies of water such as harbors (Galveston, TX), lakes (Great Lakes, Lake Baikal), and inland seas (Adriatic Sea, Baltic Sea, North Sea) demonstrate the existence of significant flooding hazard to adjacent coastal communities. We present a possible new passive-seismic method for seiche monitoring.

  18. Exercise distributed across day and night does not alter circadian period in humans.

    PubMed

    Cain, Sean W; Rimmer, David W; Duffy, Jeanne F; Czeisler, Charles A

    2007-12-01

    In rodents, increased activity due to running-wheel access is associated with a change in observed circadian period. In humans, exposure to exercise has failed to demonstrate similar effects on period. Methodological issues with prior studies such as light exposure during exercise, length of study, and method of measuring period confounded those evaluations of the effect of exercise on period in humans. In the present experiment, the authors examined the effect of exercise on period in 8 subjects using a 44-day within-subjects inpatient study. They used a 20-h forced desynchrony protocol, in which subjects were exposed to exercise across circadian phases under dim light conditions. Exercise consisted of three 45-min sessions per wake period on an ergometer. Target exercise intensity was ~65% of maximal heart rate. Intrinsic circadian period was measured using both core body temperature and hourly plasma melatonin samples. Consistent with previous reports, the authors find no effect of exercise on endogenous circadian period as measured by either core body temperature or melatonin. Exercise distributed across biological day and night does not appear to affect circadian period. PMID:18057328

  19. Does the 62-day X-ray periodicity come from ULX M82 X-1?

    E-print Network

    Qiu, Yanli; Guo, Jincheng; Wang, Jing

    2015-01-01

    M82 X-1 is the brightest ultraluminous X-ray source in starburst galaxy M82 and is one of the best intermediate mass black hole candidates. Previous studies based on the Rossi X-ray Timing Explorer/Proportional Counter Array (RXTE/PCA) reported a regular X-ray flux modulation of M82 with a period of 62 days, and attributed this periodic modulation to M82 X-1. However, this modulation is not necessarily from M82 X-1 because RXTE/PCA has a very poor spatial resolution of ~1 degree. In this work, we analyzed 1000 days of monitoring data of M82 X-1 from the Swift/X-ray telescope (XRT), which has a much better spatial resolution than RXTE/PCA. The periodicity distribution map of M82 reveals that the 62-day periodicity is most likely not from M82 X-1, but from the summed contributions of several periodic X-ray sources 4 arcsec southeast of M82 X-1. However, Swift/XRT is not able to resolve those periodic sources and locate the precise origin of the periodicity of M82. Thus, more long-term observations with higher s...

  20. The "Approximate 150 Day Quasi-Periodicity" in Interplanetary and Solar Phenomena During Cycle 23

    NASA Technical Reports Server (NTRS)

    Richardson, I. G.; Cane, H. V.

    2004-01-01

    A"quasi-periodicity" of approx. 150 days in various solar and interplanetary phenomena has been reported in earlier solar cycles. We suggest that variations in the occurrence of solar energetic particle events, inter-planetary coronal mass ejections, and geomagnetic storm sudden commenceents during solar cycle 23 show evidence of this quasi-periodicity, which is also present in the sunspot number, in particular in the northern solar hemisphere. It is not, however, prominent in the interplanetary magnetic field strength.

  1. The 155-day solar period in the sixteenth century and later

    NASA Technical Reports Server (NTRS)

    Silverman, S. M.

    1990-01-01

    Recent interest in short solar periods has been stimulated by the discovery of a peak period of about 150-160 days in very energetic solar flares and hard X-ray flares. Auroral data are used here as a proxy for solar activity to show the presence of the peak in data from 1570 to 1573 and in some other time periods. The data are also used to show the absence or lack of prominence of this peak at other times in the nineteenth and early twentieth centuries.

  2. Regulation of period 1 expression in cultured rat pineal

    NASA Technical Reports Server (NTRS)

    Fukuhara, Chiaki; Dirden, James C.; Tosini, Gianluca

    2002-01-01

    The aim of the present study was to investigate the in vitro expression of Period 1 (Per1), Period 2 (Per2) and arylalkylamine N-acetyltransferase (AA-NAT) genes in the rat pineal gland to understand the mechanism(s) regulating the expression of these genes in this organ. Pineals, when maintained in vitro for 5 days, did not show circadian rhythmicity in the expression of any of the three genes monitored. Norepinephrine (NE) induced AA-NAT and Per1, whereas its effect on Per2 was negligible. Contrary to what was observed in other systems, NE stimulation did not induce circadian expression of Per1. The effect of NE on Per1 level was dose- and receptor subtype-dependent, and both cAMP and cGMP induced Per1. Per1 was not induced by repeated NE - or forskolin - stimulation. Protein synthesis was not necessary for NE-induced Per1, but it was for reduction of Per1 following NE stimulation. Per1 transcription in pinealocytes was activated by BMAL1/CLOCK. Our results indicate that important differences are present in the regulation of these genes in the mammalian pineal. Copyright 2002 S. Karger AG, Basel.

  3. 78 FR 36560 - 30-Day Notice of Proposed Information Collection: FHA Lender Approval, Annual Renewal, Periodic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-18

    ... URBAN DEVELOPMENT 30-Day Notice of Proposed Information Collection: FHA Lender Approval, Annual Renewal...: Colette Pollard, Reports Management Officer, QDAM, Department of Housing and Urban Development, 451 7th... Title of Information Collection: FHA Lender Approval, Annual Renewal, Periodic Updates and...

  4. Characteristics of atmospheric disturbances with a quasi-two day period

    NASA Technical Reports Server (NTRS)

    Kalchenko, B. V.

    1987-01-01

    In 1968 the Soviet equatorial meteor expedition measured, at an altitude of 80 to 105 km, wind velocity disturbances in the equatorial atmosphere with a quasi-two day period. An example of such disturbances is provided which shows time series of wind velocity in the meridional and zonal directions. The results of the time series analysis are discussed.

  5. Eastward propagating planetary waves with periods of 15 days in the winter Antarctic stratosphere

    E-print Network

    Chu, Xinzhao

    Eastward propagating planetary waves with periods of 1­5 days in the winter Antarctic stratosphere that barotropic/baroclinic instability at 50°S­60°S induced by the stratospheric polar night jet and to the meridional gradient of background temperature. The migrating diurnal tide near 78°S in the upper stratosphere

  6. 19 CFR 141.82 - Invoice for installment shipments arriving within a period of 10 days.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Invoice for installment shipments arriving within a period of 10 days. 141.82 Section 141.82 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) ENTRY OF MERCHANDISE Invoices § 141.82 Invoice for installment shipments...

  7. The power spectrum of the solar wind speed for periods greater than 10 days

    NASA Technical Reports Server (NTRS)

    Fenimore, E. E.; Asbridge, J. R.; Bame, S. J.; Feldman, W. C.; Gosling, J. T.

    1978-01-01

    The use of the more than 11 years of solar wind speed data obtained by Vela 2-6 and Imp 6-8 to study the power spectrum of speed variations in the range near the solar rotational frequency is discussed. The broad bands of power near periods of 27 days (corresponding to the rotational period of the sun), 13.5 days, and higher harmonics are characterized, and it is suggested that the described individual peaks in both the solar wind and the geomagnetic spectra are probably not due to differential rotation. The alternate explanation is that the multipeak nature of the power spectra are explained by a wave packet concept in which recurring highspeed streams are described as a series of pulses (separated by a constant period) that last for a varying number of solar rotations.

  8. 75 FR 1656 - Draft Safety Culture Policy Statement: Request for Public Comments; Extension of Comment Period

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-12

    ...NUCLEAR REGULATORY COMMISSION...NRC-2009-0485] Draft Safety Culture Policy Statement...Comment Period AGENCY: Nuclear Regulatory Commission...Issuance of draft safety culture policy statement...unique aspects of nuclear safety and security,...

  9. Growth of mallards fed phosphamidon for 13-day periods during three different developmental stages

    USGS Publications Warehouse

    Haseltine, S.; Hensler, G.L.

    1981-01-01

    Mallard ducklings (Anas platyrhynchos) were exposed to a 13-day dietary treatment of O, 0.5, or 5.0 ppm phosphamidon at one of three successive age intervals (5-17 days, 18-30 days, or 31-43 days) during a 10-week growth period. Weekly measurements of body weight, wing length, primary feather length, and bill length revealed slower development of primary feathers in those birds treated from 5 to 17 days; treatment effects on body weight and wing length from 6 to 8 weeks of age were observed among those birds treated from 18 to 30 days of age. Some differences in growth patterns among birds treated with the same phosphamidon level, but at different growth stages, were attributed to the varying size of the group with which a duckling was housed at different times in the growth process. No brain cholinesterase depression was observed in any group either 24 h after phosphamidon treatment was terminated or at 10 weeks of age.

  10. The dynamic response of visual accommodation over a seven-day period

    NASA Technical Reports Server (NTRS)

    Randle, R. J.; Murphy, M. R.

    1974-01-01

    Four college students, ranging in age from 18 to 21 years, were tested on their dynamic, monocular accommodation responses to a square wave stimulus and sine waves of two frequencies. The tests were conducted over a period of seven days in a controlled environment, each subject being tested once every three hours. Latency, magnitude, velocity, gain and phase lag of the responses were measured, and means and standard deviations were computed. The latency of response was stable throughout and agreed fairly well with previous studies. The response magnitude was relatively stable. Three of the subjects had higher velocities on receding targets; one was faster on approaching targets. The group mean velocity increased over the seven days of the study. In keeping with the trend to faster dynamics over the seven days, both gain and phase lag improved.

  11. Keratinocyte dysplasia in hematopoietic stem cell transplantation recipients in the day-28-to-84 posttransplantation period.

    PubMed

    Li, Ning; Guthrie, Katherine A; Storer, Barry E; Martin, Paul J; Sale, George E; Argenyi, Zsolt B; Myerson, David

    2012-08-01

    Severe keratinocyte dysplasia (SKD) has been reported as a common event in the early posttransplantation period of hematopoietic stem cell transplantation patients. The purpose of our study is to determine the possible causes of SKD during the intermediate posttransplantation period and to ascertain its prevalence in skin biopsies. Skin biopsy slides, obtained from hematopoietic stem cell transplantation recipients who were days 28 to 84 posttransplantation, were evaluated for SKD. Forty-four examples of SKD were identified in 467 slides, or 9%. Thirty-seven patients were evaluated as cases in a case-control design. SKD was strongly associated with a conditioning regimen containing busulfan with an odds ratio of 7.25 (P = .0002). In a multivariate-adjusted analysis, SKD was not associated with cyclophosphamide, fludarabine, total-body irradiation, or a nonmyeloablative conditioning regimen. SKD was not associated with clinical acute graft-versus-host disease. SKD histology gradually resolved, reaching a normal histology after an average of 241 days. This study finds that severe keratinocyte dysplasia in the period 28 to 84 days post-HSCT is strongly associated with a busulfan-conditioning regimen. PMID:22326632

  12. GENE EXPRESSION PATTERNS OF CD-1 DAY-8 EMBRYO CULTURES EXPOSED TO BROMOCHLORO ACETIC ACID

    EPA Science Inventory

    Gene expression patterns of CD-1 day-8 embryo cultures exposed to bromochloro acetic acid

    Edward D. Karoly?*, Judith E. Schmid* and E. Sidney Hunter III*
    ?Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina and *Reproductiv...

  13. Geopathology on May Day: Expressions of Culture on Hawai'i's Elementary School Stages

    ERIC Educational Resources Information Center

    Steele, Jamie Simpson

    2013-01-01

    In Hawai'i's elementary schools, May Day programs feature children adorned with flower leis, singing and dancing hula about Hawaiian culture and performing traditions from major ethnic groups who settled the islands. Using the lens of geopathology, this research questions how various groups of residents long for belonging and struggle for…

  14. 75 FR 1656 - Draft Safety Culture Policy Statement: Request for Public Comments; Extension of Comment Period

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-12

    ... Draft Safety Culture Policy Statement: Request for Public Comments; Extension of Comment Period AGENCY: Nuclear Regulatory Commission (NRC). ACTION: Issuance of draft safety culture policy statement and notice of opportunity for public comment; Extension of comment period. SUMMARY: On November 6, 2009, (74...

  15. The physical properties of giant exoplanets within 400 days of period

    NASA Astrophysics Data System (ADS)

    Santerne, Alexandre

    2015-12-01

    At a time when small planets in the habitable zone are found, not all the questions about giant planets have been answered. For example, their formation, migration and evolution are far from being fully understood. In this context, the Kepler space mission is providing unprecedented constraints to theories by probing transiting giant planets in a wide range of orbital periods. In this talk, we will present the results of a 6-year spectroscopic survey with the SOPHIE spectrograph of the transiting giant-planet candidates detected by Kepler within 400 days of period. First, we will describe the giant-planet candidate sample from the Kepler catalog and our spectroscopic observations which allowed us to screen out more than half of the candidates as false positives. Then, we will present the occurrence rate of giant planets, based on our sample cleaned from fake transiting planets, and compare it with other surveys. Finally, we will discuss the physical properties of the giant transiting planets within 400 days of period and compare them with predictions from planet-synthesis models.

  16. WASP-117b: a 10-day-period Saturn in an eccentric and misaligned orbit

    NASA Astrophysics Data System (ADS)

    Lendl, M.; Triaud, A. H. M. J.; Anderson, D. R.; Collier Cameron, A.; Delrez, L.; Doyle, A. P.; Gillon, M.; Hellier, C.; Jehin, E.; Maxted, P. F. L.; Neveu-VanMalle, M.; Pepe, F.; Pollacco, D.; Queloz, D.; Ségransan, D.; Smalley, B.; Smith, A. M. S.; Udry, S.; Van Grootel, V.; West, R. G.

    2014-08-01

    We report the discovery of WASP-117b, the first planet with a period beyond 10 days found by the WASP survey. The planet has a mass of Mp = 0.2755 ± 0.0089 MJ, a radius of Rp= 1.021_{-0.065+0.076 Rjup} and is in an eccentric (e = 0.302 ± 0.023), 10.02165 ± 0.00055 d orbit around a main-sequence F9 star. The host star's brightness (V = 10.15 mag) makes WASP-117 a good target for follow-up observations, and with a periastron planetary equilibrium temperature of Teq= 1225_{-39+36} K and a low planetary mean density (?p= 0.259_{-0.048+0.054 ?jup}) it is one of the best targets for transmission spectroscopy among planets with periods around 10 days. From a measurement of the Rossiter-McLaughlin effect, we infer a projected angle between the planetary orbit and stellar spin axes of ? = -44 ± 11 deg, and we further derive an orbital obliquity of ? = 69.6 +4.7-4.1 deg. Owing to the large orbital separation, tidal forces causing orbital circularization and realignment of the planetary orbit with the stellar plane are weak, having had little impact on the planetary orbit over the system lifetime. WASP-117b joins a small sample of transiting giant planets with well characterized orbits at periods above 8 days. Based on data obtained with WASP-South, CORALIE and EulerCam at the Euler-Swiss telescope, TRAPPIST, and HARPS at the ESO 3.6 m telescope (Prog. IDs 087.C-0649, 089.C-0151, 090.C-0540)Photometric and radial velocities are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/568/A81

  17. Cytoreductive nephrectomy and its role in the present-day period of targeted therapy

    PubMed Central

    2015-01-01

    The beneficial effect of cytoreductive nephrectomy on survival of patients with metastatic renal cell carcinoma in the immunotherapy era was based on two prospective randomized trials. Unfortunately, such evidence does not yet exist in the present-day period of targeted therapy. Despite this, cytoreductive nephrectomy remains integral in the multimodal management of patients with metastatic renal cell carcinoma. Multiple retrospective studies as well as data from prospective studies examining targeted therapy support the continued use of cytoreductive nephrectomy in the properly selected patient. Ongoing studies will hopefully fine-tune the role and timing of cytoreductive nephrectomy in the context of targeted therapy. PMID:26425142

  18. XO-5b: A Transiting Jupiter-sized Planet with a 4 day Period

    NASA Astrophysics Data System (ADS)

    Burke, Christopher J.; McCullough, P. R.; Valenti, Jeff A.; Long, Doug; Johns-Krull, Christopher M.; Machalek, P.; Janes, Kenneth A.; Taylor, B.; Fleenor, Michael L.; Foote, C. N.; Gary, Bruce L.; García-Melendo, Enrique; Gregorio, J.; Vanmunster, T.

    2008-10-01

    The star XO-5 (GSC 02959-00729, V=12.1, G8 V) hosts a Jupiter-sized, Rp=1.15+/-0.12 RJ, transiting extrasolar planet, XO-5b, with an orbital period of 4.2 days. The planet's mass, Mp=1.15+/-0.08 MJ, and surface gravity, gp=22+/-5 m s-2, are large for its orbital period compared to most other transiting planets. However, the deviation from the Mp-P relationship for XO-5b is not as large as for GJ 436b, HAT-P-2b, and XO-3b. By coincidence, XO-5 overlies the extreme H I plume that emanates from the interacting galaxy pair NGC 2444/NGC 2445 (Arp 143).

  19. Search for evidence of a clock related to the solar 154 day complex of periodicities

    NASA Technical Reports Server (NTRS)

    Sturrock, P. A.; Bai, T.

    1992-01-01

    Evidence that has recently been compiled (Bai and Sturrock 1991) indicates that the enigmatic 154-day periodicity in solar activity may be viewed as part of a complex of periodicities that are approximate multiples of 25.8 days, suggesting that the Sun contains a 'clock' with frequency in the range 440 to 463 nano Hz. The clock may comprise either an oscillator or a rotator, each of which may be either real or virtual. We have reconsidered a previous spectrum analysis of the Zurich sunspot-number sequence by Knight, Schatten, and Sturrock (1979) which revealed a sharp, persistent and significant periodicity with a period of 12.072 days, corresponding to a frequency of about 958.8 nano Hz. This periodicity may be regarded as the (second) upper sideband of the second harmonic (2nu(sub R) + 2nu(sub E)) of a fundamental frequency of 447.7 nano Hz that is clearly within the search band. In this expression, nu(sub R) is the sidereal frequency of the hypothetical rotator and nu(sub E) is the frequency (31.69 nano Hz) of the Earth in its orbital motion around the Sun. In analyzing sunspot area data derived from the Greenwich data set, and on noting that any frequency is defined only to within the Nyquist frequency, we find clear evidence not only for the upper sideband of the second harmonic, but also for the second harmonic (2nu(sub R)) and the lower sideband of the second harmonic (2nu(sub R) - 2nu(sub E)). There is no strong peak at the fundamental frequency in the Greenwich data, but there is in the Zurich sunspot data. The effect of a linear oscillator is, to the lowest order in the amplitude, the same as the combined effect of two rotators of opposite polarities. A rotator that has arbitrary orientation with respect to the ecliptic may influence the outer layers of the Sun and thereby modulate the occurrence of solar activity such as sunspots. By analyzing a simple model, we find that such a rotator would influence surface activity in such a way that the spectrum of a 'signal' (such as the record of sunspots), as seen from the Earth, would contain components with frequencies that are certain integral combinations of nu(sub R) and nu(sub E). The amplitudes of the various components depend sensitively on theta, the angle between the axis of the rotator and the axis of the Earth's orbital motion. This simple model therefore offers a kinematical (but not dynamical) interpretation of the sunspot spectrum. The present analysis, while offering support of our conjectures that the Sun contains a clock that regulates the 154-day complex of periodicities, cannot distinguish between an osillator or a rotator (that might be a traveling wave), nor between a real rotator or a virtual rotator (that might be an apparent traveling wave due to the aliasing effect of an oscillator in a rotating system). Further analysis of sunspot and other data sets will be required to confirm the existence of such clock and (if it is real) to determine its physical nature.

  20. Mediterraneità and modernità : architecture and culture during the period of Italian colonization of North Africa

    E-print Network

    McLaren, Brian L. (Brian Lloyd), 1958-

    2001-01-01

    This dissertation examines the intersection of the modern and the colonial in architecture and culture during the period of Italian colonization of North Africa from 1911 to 1943. Rather than see the colonies as merely a ...

  1. Case studies of multi-day 3He-rich solar energetic particle periods

    NASA Astrophysics Data System (ADS)

    Chen, Nai-hwa; Bu?ík, Radoslav; Innes, Davina E.; Mason, Glenn M.

    2015-08-01

    Context. Impulsive solar energetic particle events in the inner heliosphere show the long-lasting enrichment of 3He. Aims: We study the source regions of long-lasting 3He-rich solar energetic particle (SEP) events Methods: We located the responsible open magnetic field regions, we combined potential field source surface extrapolations with the Parker spiral, and compared the magnetic field of the identified source regions with in situ magnetic fields. The candidate open field regions are active region plages. The activity was examined by using extreme ultraviolet images from the Solar Dynamics Observatory (SDO) and STEREO together with radio observations from STEREO and WIND. Results: Multi-day periods of 3He-rich SEP events are associated with ion production in single active region. Small flares or coronal jets are their responsible solar sources. We also find that the 3He enrichment may depend on the occurrence rate of coronal jets.

  2. Search for the 531 day-period wobble signal in the polar motion based on EEMD

    NASA Astrophysics Data System (ADS)

    Ding, H.; Shen, W. B.

    2015-04-01

    In this study, we use a nonlinear and non-stationary time series analysis method, the ensemble empirical mode decomposition method (EEMD), to analyze the polar motion (PM) time series (EOP C04 series from 1962 to 2013) to find a 531 day-period wobble (531 dW) signal. The 531 dW signal has been found in the early PM seires (1962-1977) while cannot be found in the recent PM seires (1978-2013) using conventional analysis approaches. By the virtue of the demodulation feature of EEMD, the 531 dW can be confirmed to be present in PM based on the differences of the amplitudes and phases between different intrinsic mode functions. Results from three sub-series divided from the EOP C04 series show that the period of the 531 dW is subject to variations, in the range of 530.9-524 d, and its amplitude is also time-dependent (about 2-11 mas). Synthetic tests are carried out to explain why the 531 dW can only be observed in recent 30-years PM time series after using EEMD. The 531 dW is also detected in two longest available superconducting gravimeter (SG) records, which further confirms the presence of the 531 dW. The confirmation of 531 dW existence could be significant in establishing a more reasonable Earth rotation model and may effectively contribute to the prediction of the PM and its mechanism interpretation.

  3. Responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata under 10-day exposure to cadmium chloride.

    PubMed

    Latire, Thomas; Le Pabic, Charles; Mottin, Elmina; Mottier, Antoine; Costil, Katherine; Koueta, Noussithé; Lebel, Jean-Marc; Serpentini, Antoine

    2012-03-01

    Among metals, cadmium, a non-essential element, is an important pollutant that is released into aquatic environments. Due to its persistence and bioaccumulation, this metal has been shown to exert immunological effects on organisms. The objective of the present study was to investigate the in vitro effects of cadmium chloride using a haemocyte primary culture from the European abalone, Haliotis tuberculata. Most studies have maintained viable haemocytes in vitro for periods ranging from several hours to several days during acute exposures. Few investigations have reported the effects of metals using longer in vitro exposures, which are more realistic with regard to mimicking environmental conditions. In this study, we exposed abalone haemocytes to concentrations from 0.5 to 50,000 ?gL(-1) of CdCl2 for 10 days. The effects of cadmium chloride were reflected in a significant decrease in the number of viable cells and morphological modifications in a concentration-dependent manner beginning at a concentration of 500 ?gL(-1) as well as in some physiological processes, such as phagocytotic activity and the number of lysosome-positive cells. In contrast, phenoloxidase (PO) activity and reactive oxygen species (ROS) production were increased beginning at a concentration of 5 ?gL(-1), which is consistent with environmental concentrations in polluted sites. For PO activity and ROS production, maximally 9-fold and 130% inductions, respectively, were recorded under the highest dose. These results thus indicate that cadmium chloride alters immune parameters of abalone haemocytes and that the long-term (10 days) primary culture system used here represents a suitable, sensitive in vitro model for assessing cytotoxic responses. PMID:22018399

  4. 76 FR 6794 - 30-Day Submission Period for Requests for ONC-Approved Accreditor (ONC-AA) Status

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-08

    ...Requests for ONC-Approved Accreditor (ONC-AA) Status AGENCY: Office of the National...requests for ONC-Approved Accreditor (ONC-AA) status. Authority: 42 U.S.C. 300jj-11...30-day period during which requests for ONC-AA status may be submitted. The 30-day...

  5. 76 FR 6794 - 30-Day Submission Period for Requests for ONC-Approved Accreditor (ONC-AA) Status

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-08

    ... Program for Health Information Technology, 76 FR 1262 (Jan. 7, 2011) (the ``Permanent Certification... SERVICES 30-Day Submission Period for Requests for ONC-Approved Accreditor (ONC-AA) Status AGENCY: Office... ONC-Approved Accreditor (ONC-AA) status. Authority: 42 U.S.C. 300jj-11. DATES: The 30-day...

  6. 76 FR 33395 - 30-Day Notice of Proposed Information Collections: ECA Sports & Culture Evaluation Surveys

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ...Proposed Information Collections: ECA Sports & Culture Evaluation Surveys ACTION... Title of Information Collection: Sports & Culture Evaluation, Between the Lines... Title of Information Collection: Sports & Culture Evaluation, Sports Envoys...

  7. CTEPP NC DATA COLLECTED ON FORM 10 (PERIODS 1-3): DAY CARE CENTER CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data set contains data concerning the child’s activities at the day care center over the 48-h monitoring period. The diary was divided into three time periods over the 48-h monitoring interval. The Food Survey collected information on the frequency and types of fruits, veget...

  8. CTEPP DATA COLLECTION FORM 10 (PERIODS 1-3): DAY CARE CENTER CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form collects information on the child's activities at the day care center over the 48-hr monitoring period. The diary is divided into three time periods over the 48-monitoring interval. The Food Survey collects information on the frequency and types of frui...

  9. Voids and Question Marks in the Present-day Data Concerning the Rotation Period of the First 1000 Numbered Asteroids

    NASA Astrophysics Data System (ADS)

    Alvarez, Eduardo Manuel

    2015-04-01

    Currently, there are only 19 three-digit numbered asteroids - none of them within the first 500 - for which their rotation period is unknown. Chances are that all of the first 1000 asteroids will have a known period in just a few years. However, not all of the 981 present-day published rotation period data for asteroids numbered below 1000 are secure. Ongoing investigations to verify, refine, or revise existing periods remains an important endeavor, especially for the 17 asteroids for which the period is currently uncertain.

  10. "A Day in the Life": Advancing a Methodology for the Cultural Study of Development and Learning in Early Childhood

    ERIC Educational Resources Information Center

    Gillen, Julia; Cameron, Catherine Ann; Tapanya, Sombat; Pinto, Giuliana; Hancock, Roger; Young, Susan; Gamannossi, Beatrice Accorti

    2007-01-01

    This paper explores the methodology of an ecological investigation of aspects of culture in the interactional construction of early childhood in diverse global communities: Peru, Italy, Canada, Thailand, and the United Kingdom. Regarding culture as a dynamic dimension of the child's socialisation, the approach taken was to film a "day in the life"…

  11. Instability of the 13.8 day period in X-ray emission from 4U1700-377

    E-print Network

    JaeSub Hong; Charles J. Hailey

    2003-09-23

    We present a new result on long-term periodicity searches of the X-ray emission from 4U1700-377 using RXTE ASM and CGRO BATSE data. The hard X-ray data (20 - 200 keV) from early BATSE observations (1780 days from JD 2448370, before RXTE observations started) show evidence of a 13.8 day periodicity. The long term periodicity became substantially less prominent in the data from later BATSE observations and in the soft X-ray data from the ASM (2 - 10 keV) observations. We demonstrate that disk precession models can explain the 13.8 day period and its instability in the X-ray emission from 4U1700-377.

  12. Discovery of a 115 Day Orbital Period in the Ultraluminous X-ray Source NGC 5408 X-1

    NASA Technical Reports Server (NTRS)

    Strohmayer, Tod E.

    2009-01-01

    We report the detection of a 115 day periodicity in SWIFT/XRT monitoring data from the ultraluminous X-ray source (ULX) NGC 5408 X-1. Our o ngoing campaign samples its X-ray flux approximately twice weekly and has now achieved a temporal baseline of ti 485 days. Periodogram ana lysis reveals a significant periodicity with a period of 115.5 +/- 4 days. The modulation is detected with a significance of 3.2 x 10(exp -4) . The fractional modulation amplitude decreases with increasing e nergy, ranging from 0.13 +/- 0.02 above 1 keV to 0.24 +/- 0.02 below 1 keV. The shape of the profile evolves as well, becoming less sharply peaked at higher energies. The periodogram analysis is consistent wi th a periodic process, however, continued monitoring is required to c onfirm the coherent nature of the modulation. Spectral analysis indic ates that NGC 5408 X-1 can reach 0.3 - 10 keV luminosities of approxi mately 2 x 10 40 ergs/s . We suggest that, like the 62 day period of the ULX in M82 (X41.4-1-60), the periodicity detected in NGC 5408 X-1 represents the orbital period of the black hole binary containing the ULX. If this is true then the secondary can only be a giant or super giant star.

  13. DISCOVERY OF A 115 DAY ORBITAL PERIOD IN THE ULTRALUMINOUS X-RAY SOURCE NGC 5408 X-1

    SciTech Connect

    Strohmayer, Tod E.

    2009-12-01

    We report the detection of a 115 day periodicity in Swift/X-Ray Telescope monitoring data from the ultraluminous X-ray source (ULX) NGC 5408 X-1. Our ongoing campaign samples its X-ray flux approximately twice weekly and has now achieved a temporal baseline of approx 485 days. Periodogram analysis reveals a significant periodicity with a period of 115.5 +- 4 days. The modulation is detected with a significance of 3.2 x 10{sup -4}. The fractional modulation amplitude decreases with increasing energy, ranging from 0.13 +- 0.02 above 1 keV to 0.24 +- 0.02 below 1 keV. The shape of the profile evolves as well, becoming less sharply peaked at higher energies. The periodogram analysis is consistent with a periodic process, however, continued monitoring is required to confirm the coherent nature of the modulation. Spectral analysis indicates that NGC 5408 X-1 can reach 0.3-10 keV luminosities of approx 2 x 10{sup 40} erg s{sup -1}. We suggest that, like the 62 day period of the ULX in M82 (X41.4+60), the periodicity detected in NGC 5408 X-1 represents the orbital period of the black hole binary containing the ULX. If this is true then the secondary can only be a giant or supergiant star.

  14. Periodical batch culture of the immobilized growing fungi Sporotrichum cellulophilum producing cellulase in the nonwoven materials

    SciTech Connect

    Tamada, M.; Kasai, N.; Kumakura, M.; Kaetsu, I.

    1986-08-01

    The thermophilic fungus Sporotrichum cellulophilum was immobilized with nonwoven materials for cellulase production. The cellulose powder concentration in the medium was an important factor controlling cellulase production. When the cellulase powder concentration in the nonwoven materials was more than 4%, cellulase production was suppressed. The growth of the immobilized fungi depended on the spaces in the nonwoven materials. Immobilized growing fungi were retained by the nonwoven materials, and the supernatant medium did not contain mycelia. The heat stability of the immobilized growing fungus was higher than that of the free fungus. The immobilized fungus gave the same FPA as the free mycelium, but the lag time for cellulase production in the immobilized fungus was longer. It was necessary for the medium to be changed in order to get the immobilized growing fungus to continue producing cellulase. In this instance there was no difference of lag time in comparsion with the free cells, and the supply of cellulose powder and polypepton was reduced to two-thirds. After 23 exchanges of the medium (2.6 mg cellulose powder/1 cubic cm nonwoven materials) FPA value was maintained. The periodic batch culture was continued for 69 days. 12 references.

  15. 78 FR 36560 - 30-Day Notice of Proposed Information Collection: FHA Lender Approval, Annual Renewal, Periodic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-18

    ...DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No...Lender Approval, Annual Renewal, Periodic Updates and...Department of Housing and Urban Development, 451 7th Street...Lender Approval, Annual Renewal, Periodic Updates...

  16. 75 FR 60490 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-30

    ...Information Collection: Bureau of Educational and Cultural Affairs, Office...State is seeking Office of Management and Budget (OMB) approval...Information Collection: Bureau of Educational and Cultural Affairs, Office...Originating Office: Bureau of Educational and Cultural Affairs,...

  17. 76 FR 16031 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... and Evaluation, Evaluation Division: Sports & Culture Evaluation Kennedy Center Visitors Survey, OMB... Policy and Evaluation, Evaluation Division: Sports & Culture Evaluation Kennedy Center Visitors Survey... larger Sports and Culture Evaluation to conduct a survey of exchange participants who participated in...

  18. 76 FR 16033 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...and Evaluation, Evaluation Division: Sports & Culture Evaluation IVLP Survey, OMB...and Evaluation, Evaluation Division: Sports & Culture Evaluation IVLP Survey. OMB...allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct a...

  19. 76 FR 16030 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...and Evaluation, Evaluation Division: Sports & Culture Evaluation Kennedy Center Mentor...and Evaluation, Evaluation Division: Sports & Culture Evaluation Kennedy Center Mentor...allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct a...

  20. 76 FR 16033 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...and Evaluation, Evaluation Division: Sports & Culture Evaluation BTL Survey, OMB...and Evaluation, Evaluation Division: Sports & Culture Evaluation BTL Survey. OMB...allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct a...

  1. 76 FR 16031 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...and Evaluation, Evaluation Division: Sports & Culture Evaluation Kennedy Center Visitors...and Evaluation, Evaluation Division: Sports & Culture Evaluation Kennedy Center Visitors...allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct a...

  2. 76 FR 16032 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...and Evaluation, Evaluation Division: Sports & Culture Evaluation IWP Survey, OMB...and Evaluation, Evaluation Division: Sports & Culture Evaluation IWP Survey. OMB...allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct a...

  3. Results of 5-year photometric monitoring of the intermediate polar V2306 Cygni: correction of the orbital period and evidence of 2-day periodicity

    NASA Astrophysics Data System (ADS)

    Breus, V.; Petrík, K.; Zo?a, S.; Baransky, A.; Hegedus, T.

    2015-09-01

    We present the results of 6 years of photometric monitoring of the magnetic cataclysmic variable V2306 Cygni (formerly known as 1WGAJ1958.2+3232) obtained at collaborating observatories. Using (O-C) analysis we tried to study variability of the spin period of the white dwarf, however we cannot make a firm conclusion based on the scatter. Simultaneously, using (O-C) diagram of orbital minima, we found that the value of 0.181545(3) days better corresponds with the light curve, than do previously published orbital period values. We also found that the variability has a 2.01 day period; this variability may be interpreted as possible precession of the accretion disk in this system.

  4. 78 FR 17313 - Ninety-Day Waiting Period Limitation and Technical Amendments to Certain Health Coverage...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    ...competition, jobs, the environment, public health or safety, or State, local...Facts. A group health plan provides that only employees with job title M are eligible...period while B is working in job title L and...Facts. A group health plan provides that only employees with job title M are eligible...period while B is......

  5. 26 CFR 301.6332-3 - The 21-day holding period applicable to property held by banks.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of this section, the term “bank” means— (1) A bank or trust company or domestic building and loan... of A's contract with X Bank up through April 23, 1992, (the last day of the holding period). Example... receiving deposits and making loans and discounts, or of exercising fiduciary powers similar to...

  6. 75 FR 4840 - Endangered and Threatened Wildlife and Plants; Indiana Bat; 30-Day Scoping Period for a National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-29

    ...Threatened Wildlife and Plants; Indiana Bat; 30- Day Scoping Period for a National...incidental take permit (ITP) for the Indiana bat (Myotis sodalis) at a wind power project...Biological information concerning the Indiana bat; (2) Relevant data concerning wind...

  7. MyUNM Medical Dental Vision Enrollment Instructions During your initial 31 calendar day benefit enrollment period

    E-print Network

    New Mexico, University of

    MyUNM Medical Dental Vision Enrollment Instructions During your initial 31 calendar day benefit enrollment period: Access Enrollment via MyUNM, LoboWeb ­ Steps 1 ­ 6 Enroll in coverage (medical, dental, select "Health" group or "Start Enrollment" to make medical, dental, or vision coverage elections. 10

  8. Finding a 24 Day Orbital Period for the X-Ray Binary 1A 1118-616

    NASA Technical Reports Server (NTRS)

    Staubert, R.; Pottschmidt, K.; Doroshenko, V.; Wilms, J.; Suchy, S.; Rothschild, R.; Santangelo, A.

    2010-01-01

    We report the first determination of the binary period and the orbital ephemeris of the Be X-ray binary containing the pulsar IA 1118-616 (35 years after the discovery of the source). The orbital period is found to be P(sub orb) = 24.0+/-0.4 days. The source was observed by RXTE during its last big X-ray outburst in January 2009, peaking at MJD 54845.4. This outburst was sampled by taking short observations every few days, covering an elapsed time comparable to the orbital period. Using the phase connection technique, pulse arrival time delays could be measured and an orbital solution determined. The data are consistent with a circular orbit, the time of 90 degrees longitude was found to be T,/2 = MJD 54845.37(10), coincident with the peak X-ray flux.

  9. Impact of Magnet Culture in Maintaining Quality Outcomes During Periods of Organizational Transition.

    PubMed

    Gonzalez, Judith F Zedreck; Wolf, Gail; Dudjak, Linda; Jordan, Bernadette

    2015-01-01

    Organizational transition presents substantial risk to maintaining quality outcomes. The leadership style and culture present during periods of change and transition empower the frontline staff to react quickly and identify opportunities. The culture of Magnet develops the skill set that enables staff to be leaders in problem solving and identifying creative care delivery approaches. Objectives of this study were to analyze the impact of organizational transition on patient and staff satisfaction, quality, and safety in a Magnet-designated hospital and determine key factors contributing to these outcomes. PMID:25768059

  10. The Metabolomic Profile of Spent Culture Media from Day-3 Human Embryos Cultured under Low Oxygen Tension

    PubMed Central

    de los Santos, Maria José; Gámiz, Pilar; de los Santos, José María; Romero, Josep Lluís; Prados, Nicolás; Alonso, Cristina; Remohí, José; Dominguez, Francisco

    2015-01-01

    Despite efforts made to improve the in vitro embryo culture conditions used during assisted reproduction procedures, human embryos must adapt to different in vitro oxygen concentrations and the new metabolic milieu provided by the diverse culture media used for such protocols. It has been shown that the embryo culture environment can affect not only cellular metabolism, but also gene expression in different species of mammalian embryos. Therefore we wanted to compare the metabolic footprint left by human cleavage-stage embryos under two types of oxygen atmospheric culture conditions (6% and 20% O2). The spent culture media from 39 transferred and implanted embryos from a total of 22 patients undergoing egg donation treatment was analyzed; 23 embryos came from 13 patients in the 6% oxygen concentration group, and 16 embryos from 9 patients were used in the 20% oxygen concentration group. The multivariate statistics we used in our analysis showed that human cleavage-stage embryos grown under both types of oxygen concentration left a similar metabolic fingerprint. We failed to observe any change in the net depletion or release of relevant analytes, such as glucose and especially fatty acids, by human cleavage-stage embryos under either type of culture condition. Therefore it seems that low oxygen tension during embryo culture does not alter the global metabolism of human cleavage-stage embryos. PMID:26562014

  11. 76 FR 16029 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...and Evaluation, Evaluation Division: Sports & Culture Evaluation Envoys Survey, OMB...and Evaluation, Evaluation Division: Sports & Culture Evaluation Envoys Survey OMB...Respondents: Envoy participants of the Sports Envoys Program conducted by ECA from...

  12. 76 FR 16031 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...of Policy and Evaluation, Evaluation Division: Sports & Culture Evaluation Sports Surveys, OMB Control Number 1405-xxxx ACTION...of Policy and Evaluation, Evaluation Division: Sports & Culture Evaluation Sports Surveys. OMB...

  13. The role of 3D microenvironmental organization in MCF-7 epithelial–mesenchymal transition after 7 culture days

    SciTech Connect

    Foroni, Laura; Vasuri, Francesco; Valente, Sabrina; Gualandi, Chiara; Focarete, Maria Letizia; Caprara, Giacomo; Scandola, Mariastella; D'Errico-Grigioni, Antonia; Pasquinelli, Gianandrea

    2013-06-10

    We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7. Scanning electron microscopy was performed at culture days 3 and 7. Immunohistochemistry (for E-cadherin, ?-catenin, cytokeratins, nucleophosmin, tubulin, Ki-67 and vimentin), immunofluorescence (for F-actin) western blot (for E-cadherin, ?-catenin and vimentin) and transmission electron microscopy were carried out at day 7. An EMT gene array followed by PCR analysis confirmed the regulation of selected genes. At day 7, scanning electron microscopy on aligned-PLA revealed spindle-shaped cells gathered in buds and ribbon-like structures, with a higher nucleolar/nuclear ratio and a loss in E-cadherin and ?-catenin at immunohistochemistry and western blot. An up-regulation of SMAD2, TGF-?2, TFPI2 and SOX10 was found in aligned-PLA compared to random-PLA cultured cells. The topography of the extracellular matrix has a role in tumor EMT, and a more aggressive phenotype characterizes MCF-7 cells cultured on aligned-PLA scaffold. -- Highlights: • After 7 culture days an aligned-PLA scaffold induces a spindle shape to MCF-7 cells. • Despite these changes, the aligned MCF-7 cells keep an epithelial phenotype. • The extracellular environment alone influences the E-cadherin/?-catenin axis. • The extracellular environment can promote the epithelial–mesenchymal transition.

  14. 75 FR 60490 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-30

    ...: Bureau of Educational and Cultural Affairs, Office of Policy and Evaluation, Evaluation Division Survey.... Originating Office: Bureau of Educational and Cultural Affairs, Office of Policy and Evaluation, Evaluation... Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs, Office of...

  15. 76 FR 16033 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... and Evaluation, Evaluation Division: Sports & Culture Evaluation IVLP Survey, OMB Control Number 1405..., Evaluation Division: Sports & Culture Evaluation IVLP Survey. OMB Control Number: None. Type of Request: New... larger Sports and Culture Evaluation to conduct a survey of exchange participants who participated...

  16. 76 FR 16033 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... and Evaluation, Evaluation Division: Sports & Culture Evaluation BTL Survey, OMB Control Number 1405..., Evaluation Division: Sports & Culture Evaluation BTL Survey. OMB Control Number: None. Type of Request: New... collection clearance will allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct...

  17. 76 FR 16031 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... and Evaluation, Evaluation Division: Sports & Culture Evaluation Sports Surveys, OMB Control Number... Evaluation, Evaluation Division: Sports & Culture Evaluation Sports Surveys. OMB Control Number: None. Type... collection clearance will allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct...

  18. 76 FR 16032 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... and Evaluation, Evaluation Division: Sports & Culture Evaluation IWP Survey, OMB Control Number 1405..., Evaluation Division: Sports & Culture Evaluation IWP Survey. OMB Control Number: None. Type of Request: New... collection clearance will allow ECA/P/V as part of their larger Sports and Culture Evaluation to conduct...

  19. An estimate of equatorial wave energy flux at 9- to 90-day periods in the Central Pacific

    NASA Technical Reports Server (NTRS)

    Eriksen, Charles C.; Richman, James G.

    1988-01-01

    Deep fluctuations in current along the equator in the Central Pacific are dominated by coherent structures which correspond closely to narrow-band propagating equatorial waves. Currents were measured roughly at 1500 and 3000 m depths at five moorings between 144 and 148 deg W from January 1981 to March 1983, as part of the Pacific Equatorial Ocean Dynamics program. In each frequency band resolved, a single complex empirical orthogonal function accounts for half to three quarters of the observed variance in either zonal or meridional current. Dispersion for equatorial first meridional Rossby and Rossby gravity waves is consistent with the observed vertical-zonal coherence structure. The observations indicate that energy flux is westward and downward in long first meridional mode Rossby waves at periods 45 days and longer, and eastward and downward in short first meridional mode Rossby waves and Rossby-gravity waves at periods 30 days and shorter. A local minimum in energy flux occurs at periods corresponding to a maximum in upper-ocean meridional current energy contributed by tropical instability waves. Total vertical flux across the 9- to 90-day period range is 2.5 kW/m.

  20. A 154-day periodicity in the occurrence of hard solar flares?

    NASA Technical Reports Server (NTRS)

    Rieger, E.; Kanbach, G.; Reppin, C.; Share, G. H.; Forrest, D. J.; Chupp, E. L.

    1984-01-01

    An analysis of the temporal distribution of 139 solar flares monitored by the Gamma Ray Spectrometer aboard the Solar Maximum Mission is reported. It is found that, instead of being randomly distributed in time, these events have a tendency to occur in groups with a mean spacing of about 154 days (75 nHz) over the observing interval. A larger sample of flares with an X-ray classification of M 2.5 or larger recorded by the GOES satellite showed a similar regularity.

  1. INTERPRETATION OF THE 115 DAY PERIODIC MODULATION IN THE X-RAY FLUX OF NGC 5408 X-1

    SciTech Connect

    Foster, D. L.; Charles, P. A.; Holley-Bockelmann, K.

    2010-12-20

    We comment on the recent observation of a 115 day modulation in the X-ray flux of the ultraluminous X-ray source (ULX) NGC 5408 X-1, and in particular, the interpretation of this modulation as the orbital period. We suggest that this modulation may instead be due to a precessing jet, and is thus superorbital in nature. Comparing the properties of this ULX with those of the prototypical micro-quasar SS 433, we argue that NGC 5408 X-1 is very similar to SS 433: a hyper-accreting stellar-mass black hole in a shorter-period binary. If the analogy holds, the 115 day modulation is best explained by the still poorly understood physics of inner-disk/jet precession and a longer observing baseline would be able to reveal an intrinsic phase jitter that is associated with such a precession.

  2. Scale-up and optimization of an acoustic filter for 200 L/day perfusion of a CHO cell culture.

    PubMed

    Gorenflo, Volker M; Smith, Laura; Dedinsky, Bob; Persson, Bo; Piret, James M

    2002-11-20

    Acoustic cell retention devices have provided a practical alternative for up to 50 L/day perfusion cultures but further scale-up has been limited. A novel temperature-controlled and larger-scale acoustic separator was evaluated at up to 400 L/day for a 10(7) CHO cell/mL perfusion culture using a 100-L bioreactor that produced up to 34 g/day recombinant protein. The increased active volume of this scaled-up separator was divided into four parallel compartments for improved fluid dynamics. Operational settings of the acoustic separator were optimized and the limits of robust operations explored. The performance was not influenced over wide ranges of duty cycle stop and run times. The maximum performance of 96% separation efficiency at 200 L/day was obtained by setting the separator temperature to 35.1 degrees C, the recirculation rate to three times the harvest rate, and the power to 90 W. While there was no detectable effect on culture viability, viable cells were selectively retained, especially at 50 L/day, where there was a 5-fold higher nonviable washout efficiency. Overall, the new temperature-controlled and scaled-up separator design performed reliably in a way similar to smaller-scale acoustic separators. These results provide strong support for the feasibility of much greater scale-up of acoustic separations. PMID:12325152

  3. SOPHIE velocimetry of Kepler transit candidates XVII. The physical properties of giant exoplanets within 400 days of period

    E-print Network

    Santerne, A; Tsantaki, M; Bouchy, F; Hébrard, G; Adibekyan, V; Almenara, J -M; Amard, L; Barros, S C C; Boisse, I; Bonomo, A S; Bruno, G; Courcol, B; Deleuil, M; Demangeon, O; Díaz, R F; Guillot, T; Havel, M; Montagnier, G; Rajpurohit, A S; Rey, J; Santos, N C

    2015-01-01

    While giant extrasolar planets have been studied for more than two decades now, there are still some open questions such as their dominant formation and migration process, as well as their atmospheric evolution in different stellar environments. In this paper, we study a sample of giant transiting exoplanets detected by the Kepler telescope with orbital periods up to 400 days. We first defined a sample of 129 giant-planet candidates that we followed up with the SOPHIE spectrograph (OHP, France) in a 6-year radial velocity campaign. This allow us to unveil the nature of these candidates and to measure a false-positive rate of 54.6 +/- 6.5 % for giant-planet candidates orbiting within 400 days of period. Based on a sample of confirmed or likely planets, we then derive the occurrence rates of giant planets in different ranges of orbital periods. The overall occurrence rate of giant planets within 400 days is 4.6 +/- 0.6 %. We recover, for the first time in the Kepler data, the different populations of giant plan...

  4. Planktonic and biofilm community characterization and Salmonella resistance of 14-day old chicken cecal microflora derived continuous-flow cultures

    Technology Transfer Automated Retrieval System (TEKTRAN)

    These studies were initiated to compare the composition of GIT bacterial communities in birds during the transition period in age where their susceptibility to Salmonella shifts to resistance. One of the challenges to developing probiotics is to develop an efficacious culture of minimal diversity, ...

  5. 77 FR 42790 - Culturally Significant Objects Imported for Exhibition Determinations: “The Last Days of Pompeii...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ...Imported for Exhibition Determinations: ``The Last Days of Pompeii: Decadence, Apocalypse, Resurrection'' SUMMARY: Notice...objects to be included in the exhibition ``The Last Days of Pompeii: Decadence, Apocalypse, Resurrection,'' imported...

  6. 76 FR 33395 - 30-Day Notice of Proposed Information Collections: ECA Sports & Culture Evaluation Surveys

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... of Policy and Evaluation, Evaluation Division (ECA/P/ V). Form Number: Survey numbers generated as... and Cultural Affairs, Office of Policy and Evaluation, Evaluation Division (ECA/P/ V). Form Number...: Bureau of Educational and Cultural Affairs, Office of Policy and Evaluation, Evaluation Division...

  7. 76 FR 16030 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... Policy and Evaluation, Evaluation Division: Sports & Culture Evaluation Kennedy Center Mentor Survey. OMB... Cultural Affairs, Office of Policy and Evaluation, Evaluation Division (ECA/P/ V). Form Number: Survey..., 2011. Julianne Paunescu, Acting Director of the Office of Policy and Evaluation, Bureau of...

  8. Clouds in North Polar Region Tracked by MOC Over Five Day Period

    NASA Technical Reports Server (NTRS)

    1999-01-01

    It is mid-summer in the northern hemisphere of Mars--a time of enhanced heating that leads to the release of water vapor into the atmosphere. In the north polar region, temperature differences between bright areas of year-round ice and dark areas of sand and rock create strong winds that mix the atmosphere and create waves of clouds that swirl around the polar cap. Sometimes, as seen during the Viking mission, these winds form tight cyclones; other times, they weave an intricate pattern reflecting the turbulence of the circulation of the atmosphere.

    This animation shows four days of observations of a representative portion of the northern hemisphere. Five Mars Orbiter Camera (MOC) wide angle image pairs(red/blue filters) were combined. These image pairs were warped to create a polar stereographic map projection, which is used by cartographers to present polar areas as if viewed from above. The edges of the pictures move back and forth because of the slightly different path taken by the Mars Global Surveyor spacecraft on each of the five orbits used in this sequence. Seen in the right-hand side of the image is the permanent ice cap and the dark areas that surround it.

    The motion of the clouds viewed in this image is typical for this season on Mars, and shows forms often seen on Earth. Waves of clouds are moving from the upper portion of the frame towards the bottom (towards the east northeast). This motion is most likely the movement of a moister portion of the martian atmosphere under the influence of circumpolar winds. Early in the sequence, a prominent circular band of clouds moves almost due east, rotating slightly counter-clockwise. Towards the end of the sequence, the circle dissipates and a linear set of clouds propagates towards the bottom of the frame. Linear cloud speeds vary from day today, averaging about 16 km/hr (10 miles/hr); rotational rates appear to have been less than 10 km/hr (6 miles/hr).

    Malin Space Science Systems and the California Institute of Technology built the MOC using spare hardware from the Mars Observer mission. MSSS operates the camera from its facilities in San Diego, CA. The Jet Propulsion Laboratory's Mars Surveyor Operations Project operates the Mars Global Surveyor spacecraft with its industrial partner, Lockheed Martin Astronautics, from facilities in Pasadena, CA and Denver, CO.

  9. Spin-orbit alignment for 110 day period KOI368.01 from gravity darkening

    SciTech Connect

    Ahlers, John P.; Seubert, Shayne A.; Barnes, Jason W.

    2014-05-10

    We fit the Kepler photometric light curve of the KOI-368 system using an oblate, gravity-darkened stellar model in order to constrain its spin-orbit alignment. We find that the system is relatively well-aligned with a sky-projected spin-orbit alignment of ? = 10° ± 2°, a stellar obliquity of ? = 3° ± 7°, and a true spin-orbit alignment of ? = 11° ± 3°. Although our measurement differs significantly from zero, the low value for ? is consistent with spin-orbit alignment. We also measure various transit parameters of the KOI-368 system: R {sub KOI-368} = 2.28 ± 0.02 R {sub ?}, R{sub p} = 1.83 ± 0.02 R {sub jup}, and i = 89.°221 ± 0.°013. This work shows that our gravity-darkened model can constrain long-period, well-aligned planets and M-class stars orbiting fast-rotators, allowing for measurement of a new subcategory of transiting bodies.

  10. Optimal Culture Incubation Time in Orthopedic Device-Associated Infections: a Retrospective Analysis of Prolonged 14-Day Incubation

    PubMed Central

    Wahl, Peter; Fracheboud, Dominique; Gautier, Emanuel

    2014-01-01

    Accurate diagnosis of orthopedic device-associated infections can be challenging. Culture of tissue biopsy specimens is often considered the gold standard; however, there is currently no consensus on the ideal incubation time for specimens. The aim of our study was to assess the yield of a 14-day incubation protocol for tissue biopsy specimens from revision surgery (joint replacements and internal fixation devices) in a general orthopedic and trauma surgery setting. Medical records were reviewed retrospectively in order to identify cases of infection according to predefined diagnostic criteria. From August 2009 to March 2012, 499 tissue biopsy specimens were sampled from 117 cases. In 70 cases (59.8%), at least one sample showed microbiological growth. Among them, 58 cases (82.9%) were considered infections and 12 cases (17.1%) were classified as contaminations. The median time to positivity in the cases of infection was 1 day (range, 1 to 10 days), compared to 6 days (range, 1 to 11 days) in the cases of contamination (P < 0.001). Fifty-six (96.6%) of the infection cases were diagnosed within 7 days of incubation. In conclusion, the results of our study show that the incubation of tissue biopsy specimens beyond 7 days is not productive in a general orthopedic and trauma surgery setting. Prolonged 14-day incubation might be of interest in particular situations, however, in which the prevalence of slow-growing microorganisms and anaerobes is higher. PMID:24153117

  11. Global Characteristics of the Correlation and Time Lag Between Solar and Ionospheric Parameters in the 27-day Period

    NASA Technical Reports Server (NTRS)

    Lee, Choon-Ki; Han, Shin-Chan; Dieter,Bilitza; Ki-Weon,Seo

    2012-01-01

    The 27-day variations of topside ionosphere are investigated using the in-situ electron density measurements from the CHAMP planar Langmuir probe and GRACE K-band ranging system. As the two satellite systems orbit at the altitudes of approx. 370 km and approx. 480 km, respectively, the satellite data sets are greatly valuable for examining the electron density variations in the vicinity of F2-peak. In a 27-day period, the electron density measurements from the satellites are in good agreements with the solar flux, except during the solar minimum period. The time delays are mostly 1-2 day and represent the hemispherical asymmetry. The globally-estimated spatial patterns of the correlation between solar flux and in-situ satellite measurements show poor correlations in the (magnetic) equatorial region, which are not found from the ground measurements of vertically-integrated electron content. We suggest that the most plausible cause for the poor correlation is the vertical movement of ionization due to atmospheric dynamic processes that is not controlled by the solar extreme ultraviolet radiation.

  12. Cultural/Favorite Recipe Day: Strengthening Approaches to Increase Culturally Diverse Foods Served in Head Start Meals

    ERIC Educational Resources Information Center

    Hoffman, Jessica A.; Agrawal, Tara; Carter, Sonia; Grinder, AnnMarie; Castaneda-Sceppa, Carmen

    2012-01-01

    One approach to halting the childhood obesity epidemic has been the modification of foods available to children during the school day. In recent years there has been an increased focus on obesity prevention efforts among children ages birth to 5 and the role of child care settings in prevention efforts. Head Start serves as an important venue for…

  13. Improvement of development of equine preantral follicles after 6 days of in vitro culture with ascorbic acid supplementation.

    PubMed

    Gomes, R G; Lisboa, L A; Silva, C B; Max, M C; Marino, P C; Oliveira, R L; González, S M; Barreiros, T R R; Marinho, L S R; Seneda, M M

    2015-09-15

    The aim of this study was to evaluate the effects of different concentrations of ascorbic acid (25, 50, and 100 ?g/mL) in supplemented minimum essential medium (MEM+) on the development of equine preantral follicles that were cultured in vitro for 2 or 6 days. The contralateral ovaries (n = 5) from five mares in seasonal anestrus were collected from a local abattoir. Nine ovarian tissue fragments of approximately 5 × 5 × 1 mm were obtained from each animal. One fragment was immediately fixed and subjected to histologic analysis (control group; Day 0), and the other eight were placed in PBS supplemented with penicillin (200 IU/mL) and streptomycin (200 mg/mL) at 4 °C for 1 hour (during transport to the laboratory). The fragments were cultured in situ for 2 days (D2) or 6 days (D6) in MEM+ or MEM+ plus ascorbic acid at three different concentrations, establishing the following nine groups: control; MEM+ (D2); MEM+ (D6); MEM+ 25 ?g/mL of ascorbic acid (D2); MEM+ 25 ?g/mL of ascorbic acid (D6); MEM+ 50 ?g/mL of ascorbic acid (D2); MEM+ 50 ?g/mL of ascorbic acid (D6); MEM+ 100 ?g/mL of ascorbic acid (D2); and MEM+ 100 ?g/mL of ascorbic acid (D6). The preantral follicles were classified according to their stage (primordial, primary, secondary, or antral) and their morphology (normal or abnormal). Slides (n = 951) including 4450 histologic sections were evaluated. Follicles were observed in only 4.85% (216 of 4450) of the histologic sections. Of the 407 follicles evaluated, 120 were in the primordial stage and 287 were in different developmental stages; additionally, 43.5% were morphologically normal. After 6 days of culture, the groups cultured with 50 and 100 ?g/mL of ascorbic acid differed in terms of follicular development compared with the other groups. On the basis of occurrence of follicular development and the presence of viable follicles, it can be concluded that a positive effect of culture for 6 days in MEM+ supplemented with 50 and 100 ?g/mL of ascorbic acid was observed on equine ovarian fragments. PMID:26074067

  14. The October-November, 2003 Solar Activity and its Relationship to the "approximately 155 day" Solar Periodicity

    NASA Technical Reports Server (NTRS)

    Richardson, I. G.; Cane, H. V.

    2005-01-01

    Periodicities of - 155 days in various solar and interplanetary phenomena were first discovered during solar cycle 21 and have been shown t o be intermittently present in other solar cycles. In the current solar cycle (23), they have been reported in solar energetic particle events and interplanetary coronal maSS ejections. We assess whether the "unexpected" October - November 2003 burst of solar activity during the late declining phase of the cycle may have been a manifestation of such a periodic behavior, and hence might have been to =me extent "predictable". If the pattern were to continue, episodes of enhanced activity might be expected around April - May and October, 2004. There was a mod- est increase activity increase in mid-April, 2004 which may conform to this pattern.

  15. The October-November, 2003 Solar Activity and its Relationship to the "approx. 155 day" Solar Periodicity

    NASA Technical Reports Server (NTRS)

    Richardson, I. G.; Cane, H. V.

    2004-01-01

    Periodicities of approx. 155 days in various solar and interplanetary phenomena were first discovered during solar cycle 21 and have been shown to be intermittently present in other solar cycles. In the current solar cycle (23), they have been reported in solar energetic particle events and interplanetary coronal mass ejections. We assess whether the "unexpected" October - November 2003 burst of solar activity during the late declining phase of the cycle may have been a manifestation of such a periodic behavior, and hence might have been to some extent "predictable". If the pattern were to continue, episodes of enhanced activity might be expected around April - May and October, 2004. There was a modest increase activity increase in mid-April, 2004 which may conform to this pattern.

  16. Use of a lactobacillus-based probiotic culture to reduce Salmonella in day of hatch broilers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A commercially available Lactobacillus probiotic (FM-B11™ Ivesco LLC) (B11) significantly reduced Salmonella recovery from day-of-hatch chicks in several studies. For all experiments, day-of-hatch male broiler chicks (n=40 per pen) were challenged with approximately 10**4 cfu per chick of Salmonell...

  17. Antimicrobial use over a four-year period using days of therapy measurement at a Canadian pediatric acute care hospital

    PubMed Central

    Dalton, Bruce R; MacTavish, Sandra J; Bresee, Lauren C; Rajapakse, Nipunie; Vanderkooi, Otto; Vayalumkal, Joseph; Conly, John

    2015-01-01

    BACKGROUND: Antimicrobial resistance is a concern that is challenging the ability to treat common infections. Surveillance of antimicrobial use in pediatric acute care institutions is complicated because the common metric unit, the defined daily dose, is problematic for this population. OBJECTIVE: During a four-year period in which no specific antimicrobial stewardship initiatives were conducted, pediatric antimicrobial use was quantified using days of therapy (DOT) per 100 patient days (PD) (DOT/100 PD) at the Alberta Children’s Hospital (Calgary, Alberta) for benchmarking purposes. METHODS: Drug use data for systemic antimicrobials administered on wards at the Alberta Children’s Hospital were collected from electronic medication administration records. DOT were calculated and rates were determined using 100 PD as the denominator. Changes over the surveillance period and subgroup proportions were represented graphically and assessed using linear regression. RESULTS: Total antimicrobial use decreased from 93.6 DOT/100 PD to 75.7 DOT/100 PD (19.1%) over the 2010/2011 through to the 2013/2014 fiscal years. During this period, a 20.0% increase in PD and an essentially stable absolute count of DOT (2.9% decrease) were observed. Overall, antimicrobial use was highest in the pediatric intensive care and oncology units. DISCUSSION: The exact changes in prescribing patterns that led to the observed reduction in DOT/100 PD with associated increased PD are unclear, but may be a topic for future investigations. CONCLUSION: Antimicrobial use data from a Canadian acute care pediatric hospital reported in DOT/100 PD were compiled for a four-year time period. These data may be useful for benchmarking purposes. PMID:26600813

  18. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...illness. (2) After entitlement, all psychiatric care days, whether in a general or a psychiatric hospital...benefit days. Accordingly, the individual has 72 days of psychiatric care (150 days less 78 days) available in the first benefit...

  19. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...illness. (2) After entitlement, all psychiatric care days, whether in a general or a psychiatric hospital...benefit days. Accordingly, the individual has 72 days of psychiatric care (150 days less 78 days) available in the first benefit...

  20. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...illness. (2) After entitlement, all psychiatric care days, whether in a general or a psychiatric hospital...benefit days. Accordingly, the individual has 72 days of psychiatric care (150 days less 78 days) available in the first benefit...

  1. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...illness. (2) After entitlement, all psychiatric care days, whether in a general or a psychiatric hospital...benefit days. Accordingly, the individual has 72 days of psychiatric care (150 days less 78 days) available in the first benefit...

  2. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...illness. (2) After entitlement, all psychiatric care days, whether in a general or a psychiatric hospital...benefit days. Accordingly, the individual has 72 days of psychiatric care (150 days less 78 days) available in the first benefit...

  3. 76 FR 16029 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...: March 4, 2011. Julianne Paunescu, Acting Director of the Office of Policy and Evaluation, Bureau of... Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs, Office of Policy... 1405-xxxx ACTION: Notice of request for public comments. SUMMARY: The Department of State is...

  4. Podcasts as a Learning Tool: German Language and Culture Every Day

    ERIC Educational Resources Information Center

    Schmidt, Johannes

    2008-01-01

    Podcasts provide a straightforward opportunity to stay connected with language, culture, and recent events of German-speaking countries. Podcasts offer clearly articulated, authentic material that can be automatically and regularly delivered to your computer and classrooms; continuously exposing students and teachers to German. This article…

  5. Using 12-day sampling periods for estimating long-term ambient air exposures to dioxins/furans

    SciTech Connect

    Mauch, S.; Virag, P.

    1997-12-31

    Long-duration and 24-hour dioxin/furan air samples were collected as part of a two-year, multi-media environmental exposure assessment for a resource recovery facility in a predominantly rural area. The extended samples were collected over 12-day periods to improve the detection frequency of the 2,3,7,8-substituted compounds used to calculate TEQ values. The long-duration samples were collected using the standard TO-9 method, with minor modifications to the sampling apparatus. The air sampling rate was not decreased relative to the 24-hour samples, and was approximately 250 L/min. The high-resolution GC/MS analytical procedures were unchanged. The 12-day samples showed a significant improvement in the frequency of detection of 2,3,7,8-substituted compounds, with no breakthrough indicated. Typical contributions to the overall TEQ values for detected compounds were about 80%, versus 10% for 24-hour samples (all TEQs were calculated using a half-detection-limit substitution for non-detected compounds). The total masses of congeners commonly detected in the 24-hour samples (e.g., OCDD, OCDF) did not increase in direct proportion with the volume sampled in the 12-day samples. The experience in this study showed that long-duration dioxins/furans sampling is a workable approach that can provide more realistic information for input into evaluations of human health risk.

  6. Analyzing the Relationship of Organizational Trust and Organizational Culture with Knowledge Sharing Behavior in Teachers of Second Intermediate Period

    ERIC Educational Resources Information Center

    Shahhosseini, Sakineh; Nadi, Mohammad Ali

    2015-01-01

    The present paper aims to study the relationship of organizational trust, organizational culture with knowledge sharing behavior among teachers of Second Intermediate Period in the City of Isfahan. Research method was correlation and statistical population included all teachers of Second Intermediate Period of Isfahan in academic year 2013-2014 (N…

  7. Effects of timing of vaccination (day 0 versus day 14 of a receiving period) with a modified-live respiratory viral vaccine on performance, feed intake, and febrile response of beef heifers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this study was to evaluate the effects of timing of the administration of a modified-live respiratory viral vaccine on day 0 or on day 14 of a receiving period on performance, feed intake, and febrile response in beef heifers. Our hypothesis was vaccine timing will alter febrile res...

  8. Comprehensive Mapping of Regional Expression of the Clock Protein PERIOD2 in Rat Forebrain across the 24-h Day

    PubMed Central

    Harbour, Valerie L.; Weigl, Yuval; Robinson, Barry; Amir, Shimon

    2013-01-01

    In mammals, a light-entrainable clock located in the suprachiasmatic nucleus (SCN) regulates circadian rhythms by synchronizing oscillators throughout the brain and body. Notably, the nature of the relation between the SCN clock and subordinate oscillators in the rest of the brain is not well defined. We performed a high temporal resolution analysis of the expression of the circadian clock protein PERIOD2 (PER2) in the rat forebrain to characterize the distribution, amplitude and phase of PER2 rhythms across different regions. Eighty-four LEW/Crl male rats were entrained to a 12-h: 12-h light/dark cycle, and subsequently perfused every 30 min across the 24-h day for a total of 48 time-points. PER2 expression was assessed with immunohistochemistry and analyzed using automated cell counts. We report the presence of PER2 expression in 20 forebrain areas important for a wide range of motivated and appetitive behaviors including the SCN, bed nucleus, and several regions of the amygdala, hippocampus, striatum, and cortex. Eighteen areas displayed significant PER2 rhythms, which peaked at different times of day. Our data demonstrate a previously uncharacterized regional distribution of rhythms of a clock protein expression in the brain that provides a sound basis for future studies of circadian clock function in animal models of disease. PMID:24124556

  9. A HIGHLY INCLINED ORBIT FOR THE 110 DAY PERIOD M-DWARF COMPANION KOI-368.01

    SciTech Connect

    Zhou, George; Huang, Chelsea X.

    2013-10-20

    We report the detection of asymmetry in the transit light curves of the 110 day period companion to KOI-368, a rapidly rotating A-dwarf. The significant distortion in the transit light curve is attributed to spin-orbit misalignment between the transiting companion and the gravity darkened host star. Our analysis was based on 11 long cadence and 2 short cadence transits of KOI-368.01 from the Kepler mission, as well as stellar parameters determined from our follow-up spectroscopic observation. We measured the true obliquity between the orbit normal and the stellar rotation axis to be 69{sub -10}{sup +9o}. We also find a secondary eclipse event with depth 29 ± 3 ppm at phase 0.59, from which the temperature of the companion is constrained to 3060 ± 50 K, indicating that KOI-368.01 is a late M-dwarf. The eccentricity is also calculated from the eclipse to be 0.1429 ± 0.0007. The long period, high obliquity, and low eccentricity of KOI-368.01 allow us to limit a number of proposed theories for the misalignment of binary systems.

  10. Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures

    PubMed Central

    Niapour, Nazila; Mohammadi-Ghalehbin, Behnam; Golmohammadi, Mohammad Ghasem; Amani, Mohammad; Salehi, Hossein; Niapour, Ali

    2015-01-01

    Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve. Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The total cell yield and the purity of SCs were evaluated in each group on the first and seventh day after plating. Results: The maximum and minimum numbers of cells were counted in 15 day-long predegene-ration and control groups which underwent no predegeneration. The 10 day-long in vitro predegeneration group with 80±0.5% SCs enrichment had the best purity after plating day and could maintain its purity with elapsing on cultures. Conclusion: 10 day-long predegeneration results in the higher cell number and the better and prolonged purity of SCs in culture. PMID:25945245

  11. CTEPP-OH DATA COLLECTED ON FORM 10 (PERIODS 1-3): DAY CARE CENTER CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data set contains data for CTEPP-OH concerning the child’s activities at the day care center over the 48-h monitoring period. The diary was divided into three time periods over the 48-h monitoring interval. The Food Survey collected information on the frequency and types of ...

  12. 43 CFR 1822.14 - What if I try to file a required document on the last day of the stated period for filing, but...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...period for filing, but the BLM office where it is to be filed is officially closed all...period for filing, but the BLM office where it is to be filed is officially closed all...considers the document timely filed if we receive it in the office on the next day it is...

  13. An orbital period of 0.94 days for the hot-Jupiter planet WASP-18b.

    PubMed

    Hellier, Coel; Anderson, D R; Cameron, A Collier; Gillon, M; Hebb, L; Maxted, P F L; Queloz, D; Smalley, B; Triaud, A H M J; West, R G; Wilson, D M; Bentley, S J; Enoch, B; Horne, K; Irwin, J; Lister, T A; Mayor, M; Parley, N; Pepe, F; Pollacco, D L; Segransan, D; Udry, S; Wheatley, P J

    2009-08-27

    The 'hot Jupiters' that abound in lists of known extrasolar planets are thought to have formed far from their host stars, but migrate inwards through interactions with the proto-planetary disk from which they were born, or by an alternative mechanism such as planet-planet scattering. The hot Jupiters closest to their parent stars, at orbital distances of only approximately 0.02 astronomical units, have strong tidal interactions, and systems such as OGLE-TR-56 have been suggested as tests of tidal dissipation theory. Here we report the discovery of planet WASP-18b with an orbital period of 0.94 days and a mass of ten Jupiter masses (10 M(Jup)), resulting in a tidal interaction an order of magnitude stronger than that of planet OGLE-TR-56b. Under the assumption that the tidal-dissipation parameter Q of the host star is of the order of 10(6), as measured for Solar System bodies and binary stars and as often applied to extrasolar planets, WASP-18b will be spiralling inwards on a timescale less than a thousandth that of the lifetime of its host star. Therefore either WASP-18 is in a rare, exceptionally short-lived state, or the tidal dissipation in this system (and possibly other hot-Jupiter systems) must be much weaker than in the Solar System. PMID:19713926

  14. Entamoeba histolytica: mechanism of decrease of virulence of axenic cultures maintained for prolonged periods.

    PubMed

    Olivos, Alfonso; Ramos, Espiridión; Nequiz, Mario; Barba, Carlos; Tello, Eusebio; Castañón, Guadalupe; González, Augusto; Martínez, Rubén D; Montfort, Irmgard; Pérez-Tamayo, Ruy

    2005-07-01

    Intraportal injection of non-virulent E. histolytica (derived from prolonged axenic culture of virulent E. histolytica) strain HM1-IMSS in normal hamsters results in no liver lesions and disappearance of the parasites 48-72 h after injection. Viability of non-virulent E. histolytica after 2 h of in vitro incubation in either fresh or decomplemented hamster serum is the same as control virulent E. histolytica (50-90%). In hamsters made leukopenic, or both leukopenic+hypocomplementemic, or hypocomplementemic+sephadex microspheres (to produce focal liver ischemia) intraportally injected non-virulent E. histolytica cause no lesions and disappear after 24 h. In addition, neither hypocomplementemia nor immunosuppression with cyclosporin A prolonged the survival of non-virulent E. histolytica. Methyl prednisolone treatment of hamsters resulted in survival of large numbers of non-virulent E. histolytica in the liver, with little inflammation and minimal tissue damage, for up to 7 days. Inflammatory cells (macrophages) would appear to be chiefly responsible for elimination of non-virulent E. histolytica. Parallel experiments with E. dispar suggest a different mechanism for its non-pathogenicity. PMID:15955329

  15. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... stay was for diagnosis or treatment of mental illness. (2) After entitlement, all psychiatric care days... for a mental condition and, after 10 days, transferred to a participating psychiatric hospital. The... mental condition. Only the 78 days spent in the psychiatric hospital during the pre-entitlement...

  16. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... stay was for diagnosis or treatment of mental illness. (2) After entitlement, all psychiatric care days... for a mental condition and, after 10 days, transferred to a participating psychiatric hospital. The... mental condition. Only the 78 days spent in the psychiatric hospital during the pre-entitlement...

  17. 42 CFR 409.63 - Reduction of inpatient psychiatric benefit days available in the initial benefit period.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... stay was for diagnosis or treatment of mental illness. (2) After entitlement, all psychiatric care days... for a mental condition and, after 10 days, transferred to a participating psychiatric hospital. The... mental condition. Only the 78 days spent in the psychiatric hospital during the pre-entitlement...

  18. Swift Reveals a ~5.7 Day Super-orbital Period in the M31 Globular Cluster X-Ray Binary XB158

    NASA Astrophysics Data System (ADS)

    Barnard, R.; Garcia, M. R.; Murray, S. S.

    2015-03-01

    The M31 globular cluster X-ray binary XB158 (a.k.a. Bo 158) exhibits intensity dips on a 2.78 hr period in some observations, but not others. The short period suggests a low mass ratio, and an asymmetric, precessing disk due to additional tidal torques from the donor star since the disk crosses the 3:1 resonance. Previous theoretical three-dimensional smoothed particle hydrodynamical modeling suggested a super-orbital disk precession period 29 ± 1 times the orbital period, i.e., ~81 ± 3 hr. We conducted a Swift monitoring campaign of 30 observations over ~1 month in order to search for evidence of such a super-orbital period. Fitting the 0.3-10 keV Swift X-Ray Telescope luminosity light curve with a sinusoid yielded a period of 5.65 ± 0.05 days, and a >5? improvement in ?2 over the best fit constant intensity model. A Lomb-Scargle periodogram revealed that periods of 5.4-5.8 days were detected at a >3? level, with a peak at 5.6 days. We consider this strong evidence for a 5.65 day super-orbital period, ~70% longer than the predicted period. The 0.3-10 keV luminosity varied by a factor of ~5, consistent with variations seen in long-term monitoring from Chandra. We conclude that other X-ray binaries exhibiting similar long-term behavior are likely to also be X-ray binaries with low mass ratios and super-orbital periods.

  19. Long-term developments in Her X-1: Correlation between the histories of the 35 day turn-on cycle and the 1.24 sec pulse period

    E-print Network

    R. Staubert; S. Schandl; D. Klochkov; J Wilms; K. Postnov; N. Shakura

    2007-02-20

    We have studied the long-term (1971-2005) behaviour of the 1.24 sec pulse period and the 35 day precession period of Her X-1 and show that both periods vary in a highly correlated way (see also Staubert et al. 1997 and 2000). When the spin-up rate decreases, the 35 day turn-on period shortens.This correlation is most evident on long time scales (~2000 days),e.g.around four extended spin-down episodes, but also on shorter time scales (a few 100 days) on which quasi-periodic variations are apparent. We argue that the likely common cause is variations of the mass accretion rate onto the neutron star.The data since 1991 allow a continuous sampling and indicate a lag between the turn-on behaviour and the spin behaviour, in the sense that changes are first seen in the spin, about one cycle later in the turn-on. Both the coronal wind model (Schandl & Meyer 1994) as well as the stream-disk model (Shakura et al.999) predict this kind of behaviour.

  20. “Nothing Special, Everything Is Maamuli”: Socio-Cultural and Family Practices Influencing the Perinatal Period in Urban India

    PubMed Central

    Raman, Shanti; Srinivasan, Krishnamachari; Kurpad, Anura; Razee, Husna; Ritchie, Jan

    2014-01-01

    Background Globally, India contributes the largest share in sheer numbers to the burden of maternal and infant under-nutrition, morbidity and mortality. A major gap in our knowledge is how socio-cultural practices and beliefs influence the perinatal period and thus perinatal outcomes, particularly in the rapidly growing urban setting. Methods and Findings Using data from a qualitative study in urban south India, including in-depth interviews with 36 women who had recently been through childbirth as well as observations of family life and clinic encounters, we explored the territory of familial, cultural and traditional practices and beliefs influencing women and their families through pregnancy, childbirth and infancy. We found that while there were some similarities in cultural practices to those described before in studies from low resource village settings, there are changing practices and ideas. Fertility concerns dominate women’s experience of married life; notions of gender preference and ideal family size are changing rapidly in response to the urban context; however inter-generational family pressures are still considerable. While a rich repertoire of cultural practices persists throughout the perinatal continuum, their existence is normalised and even underplayed. In terms of diet and nutrition, traditional messages including notions of ‘hot’ and ‘cold’ foods, are stronger than health messages; however breastfeeding is the cultural norm and the practice of delayed breastfeeding appears to be disappearing in this urban setting. Marriage, pregnancy and childbirth are so much part of the norm for women, that there is little expectation of individual choice in any of these major life events. Conclusions A greater understanding is needed of the dynamic factors shaping the perinatal period in urban India, including an acknowledgment of the health promoting as well as potentially harmful cultural practices and the critical role of the family. This will help plan culturally appropriate integrated perinatal health care. PMID:25369447

  1. Can the 62 Day X-ray Period of ULX M82 X-1 Be Due to a Precessing Accretion Disk?

    NASA Technical Reports Server (NTRS)

    Pasham, Dheeraj R.; Strohmayer, Tod E.

    2013-01-01

    We have analyzed all the archival RXTE/PCA monitoring observations of the ultraluminous X-ray source (ULX) M82 X-1 in order to study the properties of its previously discovered 62 day X-ray period (Kaaret & Feng 2007). Based on the high coherence of the modulation it has been argued that the observed period is the orbital period of the binary. Utilizing a much longer data set than in previous studies we find: (1) The phase-resolved X-ray (3-15 keV) energy spectra - modeled with a thermal accretion disk and a power-law corona - suggest that the accretion disk's contribution to the total flux is responsible for the overall periodic modulation while the power-law flux remains approximately constant with phase. (2) Suggestive evidence for a sudden phase shift-of approximately 0.3 in phase (20 days)-between the first and the second halves of the light curve separated by roughly 1000 days. If confirmed, the implied timescale to change the period is approx. = 10 yrs, which is exceptionally fast for an orbital phenomenon. These independent pieces of evidence are consistent with the 62 day period being due to a precessing accretion disk, similar to the so-called super-orbital periods observed in systems like Her X-1, LMC X-4, and SS433. However, the timing evidence for a change in the period needs to be confirmed with additional observations. This should be possible with further monitoring of M82 with instruments such as the X-ray telescope (XRT) on board Swift.

  2. 43 CFR 1822.14 - What if I try to file a required document on the last day of the stated period for filing, but...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false What if I try to file a required document on the last day of the stated period for filing, but the BLM office where it is to be filed is...) APPLICATION PROCEDURES Filing a Document with BLM § 1822.14 What if I try to file a required document on...

  3. 43 CFR 1822.14 - What if I try to file a required document on the last day of the stated period for filing, but...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What if I try to file a required document on the last day of the stated period for filing, but the BLM office where it is to be filed is...) APPLICATION PROCEDURES Filing a Document with BLM § 1822.14 What if I try to file a required document on...

  4. Variability of the quasi-2-day wave and interaction with longer period planetary waves in the MLT at Cachoeira Paulista (22.7°S, 45°W)

    NASA Astrophysics Data System (ADS)

    Guharay, A.; Batista, P. P.; Clemesha, B. R.

    2015-08-01

    An exclusive study has been carried out with long term meteor wind data (2000-2014) to characterize the quasi-2-day wave (QTDW) in the mesosphere and lower thermosphere (MLT) and its interactions with the longer period planetary waves at Cachoeira Paulista (22.7°S, 45°W). The QTDW is observed to be dominant during late summer (January-February) all the years under consideration except 2013. All the wave parameters exhibit significant interannual variability. The maximum wave amplitude comes out to be 39 m/s, which is significantly higher than the reported northern hemispheric findings. The mean MLT period exhibits a wide range of variability (36-70 h) indicating the presence of multiple Rossby normal modes with varying zonal wave numbers. Modulations of the QTDW amplitude by the planetary waves with longer periodicities (>9 days) are evident, especially during summer. The nonlinear interactions between the 2-day wave and longer period waves are believed to give rise to a host of secondary waves with frequencies lying close to 2-day. The strong QTDW activity, as observed at this location, has potential to cause significant effect on the overlying ionosphere and hence the atmosphere-ionosphere dynamical coupling.

  5. [Changes in the lysosome composition in cultured hepatocytes from 3-day-old rats with destabilization of the cytoplasmic microtubules].

    PubMed

    Stvolinskaia, N S; Korovokin, B F

    1991-01-01

    The monolayer culture of 3-day-old rat hepatocytes was treated by antimicrotubule agent colchicine after activation with isoproterenol. Alterations in the amount of lysosomes with the size of 1.0-2.5 and 3-6 mkm per cell were studied with light microscope after staining with the vital dye neutral red and after identification of acid phosphatase by plumbum precipitation in the frozen preparations. It was shown that isoproterenol application (20 microM) within 1.5 h did not change significantly the number of lysosomes sized 1.0-2.5 microns but stimulated the increase in the amount of lysosomes 3-6 mkm in size. The addition of 2.5 mM colchicine, after the isoproterenol treatment, decreases greatly the number of lysosomes with the size of 1.0-2.5 and 3-6 mkm. A considerable part of the large lysosomes can be resistant to the colchicine action. It is concluded that the microtubules play an important role in lysosome functioning. PMID:1841457

  6. The influence of protozoa with a filtered and non-filtered seawater culture of Tetraselmis sp., and effects to the bacterial and algal communities over 10 days.

    PubMed

    Erkelens, Mason; Ball, Andrew S; Lewis, David M

    2014-12-01

    In this study a filter was used to remove protozoa and its effects on a Tetraselmis sp. culture were evaluated in terms of final total lipid, final total dry weight, cell counts, and both the bacterial and algal communities. The protozoa species observed within this study was identified as Cohnilembus reniformis. It was observed that on the final day no C. reniformis were present in filtered cultures compared to the non-filtered culture which contained 40±3 C. reniformis/mL. The presence of C. reniformis within the culture did not affect the total lipid or the total dry weight recovered, suggesting that Tetraselmis sp. was capable of surviving and growing in the presence of C. reniformis. Overall it is suggested that an 11 ?m filter was effective at removing protozoa, though growing a microalgae culture without filtration did not show any significant effect. PMID:25314666

  7. Variations of the semi-diurnal tidal wind in the meteor region with periods of about 27 and 13.5 days

    NASA Technical Reports Server (NTRS)

    Greisiger, K. M.

    1987-01-01

    Daily values of sunspot number and solar radio emission at 10.7 cm wavelength show a well known strong modulation with a period of 27 days, the Sun's rotation period. Recent satellite measurements revealed the same modulation of the ultraviolet irradiance at wavelength below 300 nm. These UV variations can influence the thermal heating of the ozone layer by altering the chemical composition. Therefore, one can also expect a corresponding variation in the middle atmosphere of the semi-diurnal tide, which is thermally excited essentially by absorption of UV between 200 and 370 nm in the upper ozone layer. Results of radar meteor wind measurements were used to detect a presumable very weak 27 day modulation within the natural and artificial noise of the daily values of the semi-diurnal wind amplitude. The summer period was chosen because of its relatively steady conditions and the maximum amplitudes during the year. The results of this investigation are given and discussed.

  8. African Easterly Waves in 30-day High-Resolution Global Simulations: A Case Study During the 2006 NAMMA Period

    NASA Technical Reports Server (NTRS)

    Shen, Bo-Wen; Tao, Wei-Kuo; Wu, Man-Li C.

    2010-01-01

    In this study, extended -range (30 -day) high-resolution simulations with the NASA global mesoscale model are conducted to simulate the initiation and propagation of six consecutive African easterly waves (AEWs) from late August to September 2006 and their association with hurricane formation. It is shown that the statistical characteristics of individual AEWs are realistically simulated with larger errors in the 5th and 6th AEWs. Remarkable simulations of a mean African easterly jet (AEJ) are also obtained. Nine additional 30 -day experiments suggest that although land surface processes might contribute to the predictability of the AEJ and AEWs, the initiation and detailed evolution of AEWs still depend on the accurate representation of dynamic and land surface initial conditions and their time -varying nonlinear interactions. Of interest is the potential to extend the lead time for predicting hurricane formation (e.g., a lead time of up to 22 days) as the 4th AEW is realistically simulated.

  9. Cosmic ray variations with the period close to 27 days and their connection with solar activity longitudinal distribution

    NASA Technical Reports Server (NTRS)

    Bazilevskaya, G. A.; Tyasto, M. I.; Vernova, E. S.

    1985-01-01

    The amplitude and phase changes of the 27 day cosmic ray variation from 1958 to 1975 are studied. The comparison between cosmic ray intensity characteristics and longitudinal distribution of solar activity ones for different stages of the solar cycle are made.

  10. SWIFT OBSERVATIONS OF THE HIGH-MASS X-RAY BINARY IGR J16283-4838 UNVEIL A 288 DAY ORBITAL PERIOD

    SciTech Connect

    Cusumano, G.; Segreto, A.; La Parola, V.; D'Aì, A.; Masetti, N.; Tagliaferri, G.

    2013-09-20

    We report on the temporal and spectral properties of the high-mass X-ray binary IGR J16283-4838 in the hard X-ray band. We searched the first 88 months of Swift Burst Alert Telescope (BAT) survey data for long-term periodic modulations. We also investigated the broad band (0.2-150 keV) spectral properties of IGR J16283-4838 complementing the BAT dataset with soft X-ray data from the available Swift-XRT pointed observations. The BAT light curve of IGR J16283-4838 revealed a periodic modulation at P{sub o} = 287.6 ± 1.7 days (with a significance higher than 4 standard deviations). The profile of the light curve folded at P{sub o} shows a sharp peak lasting ?12 days over a flat plateau. The long-term light curve also shows a ?300 day interval of prolonged enhanced emission. The observed phenomenology suggests that IGR J16283-4838 has a Be nature, where the narrow periodic peaks and the ?300 day outburst can be interpreted as Type I and Type II outbursts, respectively. The broad band 0.2-150 keV spectrum can be described with an absorbed power-law and a steepening in the BAT energy range.

  11. CULTURAL ENRICHMENT THROUGH COMMUNITY ACTION PROJECT. ANNUAL REPORT FOR PERIOD ENDING JULY 1, 1967. (TITLE SUPPLIED).

    ERIC Educational Resources Information Center

    WILSON, O.J.

    WESTERN KENTUCKY UNIVERSITY INITIATED A PROJECT TO DETERMINE THE CULTURAL NEEDS AND INTERESTS OF THE AREA IT SERVES. IN PHASE ONE, CITY AND COUNTY REPRESENTATIVES ATTENDED A SYMPOSIUM ON ART, MUSIC, LIBRARY, LECTURE, AND THEATER RESOURCES AND FORMED A REGIONAL ADVISORY COUNCIL FOR COMMUNITY ENRICHMENT. PHASE TWO WAS A WORKSHOP TO HELP COUNCIL…

  12. Two-day period fluctuation of PMC occurrence over Syowa Station, Antarctica observed by a ground-based lidar and AIM satellite.

    NASA Astrophysics Data System (ADS)

    Nakamura, T.; Suzuki, H.; Tsutsumi, M.; Ejiri, M. K.; Tomikawa, Y.; Abo, M.; Kawahara, T.; Tsuda, T. T.; Nishiyama, T.

    2014-12-01

    A Rayleigh/Raman lidar system has been operated by the Japanese Antarctic Research Expedition (JARE) since February, 2011 (JARE 52nd) in Syowa Station Antarctica (69.0S, 39.5E). The lidar system consists of a pulsed Nd:YAG laser (355nm) as a transmitter and two telescopes with four photo multiplier tubes which are to detect Rayleigh scattered light from low and high atmosphere at 355 nm and N2 Raman emission at 387nm. Polar Mesospheric Cloud (PMC) was detected by the lidar at 22:30UT (+3hr for LT) on Feb 4th, 2011, the first day of a routine operation. This event was the first time to detect PMC over Syowa Station by a lidar [Suzuki et al., Ann. Geophys., 2013]. However, signal to noise ratio (SNR) of the PMC event was not so good due to large shot noises from daytime background signals. Moreover, a receiver system was designed mainly for nighttime observations. In this way, observation of PMC during the midnight sun, which also corresponds to most frequent PMC season, was difficult. Thus, to improve SNR of the PMC observation with the lidar during daytime, a narrow band-pass Fabry-Perot etalon unit has been developed and installed in the receiver system on Dec 2013 by JARE 55th. By using this new system, clear PMC signals were successfully detected under daylight condition during the period of summer operation of JARE55th. During this period of 53 days (from 17 Dec. 2013 to 7 Feb. 2014), only 11 days were with a clear sky and suitable for PMC observation. Thus, it was difficult to study temporal variations on a PMC activity only by using the lidar data. Fortunately, NASA's AIM satellite had passed near Syowa Station and provided with complimentary PMC data during observation gap of the lidar. By combining our lidar data with the AIM/CIPS data, nearly continuous monitoring of PMC variability over Syowa Station was achieved for period between 13th and 18th in January 2014. PMC occurrence with an interval of two days over Syowa Station during the period was clearly confirmed. Co-located MF radar also showed clear two days fluctuation in horizontal wind velocities around PMC altitude during the same period. In this presentation, we will discuss the cause of the two-day oscillation found in PMC occurrence and horizontal wind velocity. In particular, two-day planetary wave will be quantitatively investigated as a potential cause of the fluctuation.

  13. Earth Observing-1 Advanced Imager Flight Performance Assessment: Investigating Dark Current Stability Over One-Half Orbit Period during the First 60 Days

    NASA Technical Reports Server (NTRS)

    Mendenhall, J. A.

    2001-01-01

    The stability of the EO-1 Advanced Land Imager dark current levels over the period of one-half orbit is investigated. A series of two-second dark current collections, over the course of 40 minutes, was performed during the first sixty days the instrument was in orbit. Analysis of this data indicates only two dark current reference periods, obtained entering and exiting eclipse, are required to remove ALI dark current offsets for 99.9% of the focal plane to within 1.5 digital numbers for any observation on the solar illuminated portion of the orbit.

  14. Kepler and the seven dwarfs: detection of low-level day-time-scale periodic photometric variations in white dwarfs

    NASA Astrophysics Data System (ADS)

    Maoz, Dan; Mazeh, Tsevi; McQuillan, Amy

    2015-02-01

    We make use of the high photometric precision of Kepler to search for periodic modulations among 14 normal (DA- and DB-type, likely non-magnetic) hot white dwarfs (WDs). In five, and possibly up to seven of the WDs, we detect periodic, ˜2 h-10 d, variations, with semi-amplitudes of 60-2000 ppm, lower than ever seen in WDs. We consider various explanations: WD rotation combined with magnetic cool spots; rotation combined with magnetic dichroism; rotation combined with hotspots from an interstellar-medium accretion flow; transits by size ˜50-200 km objects; relativistic beaming due to reflex motion caused by a cool companion WD; or reflection/re-radiation of the primary WD light by a brown-dwarf or giant-planet companion, undergoing illumination phases as it orbits the WD. Each mechanism could be behind some of the variable WDs, but could not be responsible for all five to seven variable cases. Alternatively, the periodicity may arise from UV metal-line opacity, associated with accretion of rocky material, a phenomenon seen in ˜50 per cent of hot WDs. Non-uniform UV opacity, combined with WD rotation and fluorescent optical re-emission of the absorbed UV energy, could perhaps explain our findings. Even if reflection by a planet is the cause in only a few of the seven cases, it would imply that hot Jupiters are very common around WDs. If some of the rotation-related mechanisms are at work, then normal WDs rotate as slowly as do peculiar WDs, the only kind for which precise rotation measurements have been possible to date.

  15. Johannes and the seven dwarfs: Kepler detection of low-level day-timescale periodic photometric variations in white dwarfs

    E-print Network

    Maoz, Dan; McQuillan, Amy

    2014-01-01

    We make use of the high photometric precision of Kepler to search for periodic variations among 14 normal (DA- and DB-type, likely non-magnetic) hot white dwarfs (WDs) observed by the mission. In seven of the WDs, we detect periodic, ~2 hr to 10 d, variations, with semi-amplitudes of 40 to 2000 ppm, lower than ever seen in WDs. We consider various explanations for the observed variations: WD rotation combined with magnetic cool spots; rotation combined with magnetic dichroism; rotation combined with hot spots from an interstellar-medium accretion flow, channeled onto weak magnetic poles; transits by size ~50-200 km objects; relativistic beaming due to reflex motion caused by a cool companion WD; or reflection/re-radiation of the primary WD light by a brown-dwarf companion, or by a giant planet of about Jupiter radius, undergoing illumination phases as it orbits the WD. Each of these mechanisms could be behind some of the variable WDs, but could not be responsible for all seven variable cases. Alternatively, t...

  16. Integrated biological and cultural practices can reduce crop rotation period of organic strawberries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Approached by an organic grower and the land owner, a team of researchers conducted a replicated on-farm experiment with the break period between strawberry crops (continuous strawberries with broccoli residue incorporation, one year break, two year break, three year break, and seven year break) as ...

  17. Period3 VNTR polymorphism influences the time-of-day pain onset of acute myocardial infarction with ST elevation.

    PubMed

    Lipkova, Jolana; Splichal, Zbynek; Bienertova-Vasku, Julie Anna; Jurajda, Michal; Parenica, Jiri; Vasku, Anna; Goldbergova, Monika Pavkova

    2014-10-01

    It is well established that the incidence and infarct size in acute myocardial infarction (AMI) is subject to circadian variations. At the molecular level, circadian clocks in distinct cells, including cardiomyocytes, generate 24-h cycles of biochemical processes. Possible imbalance or impairment in the cell clock mechanism may alter the cardiac metabolism and function and increase the susceptibility of cardiovascular diseases. One of the key components of the human clock system PERIOD3 (PER3) has been recently demonstrated to affect circadian expression of various genes in different tissues, including the heart. The variable number tandem repeat (VNTR) polymorphism (rs57875989) in gene Period3 (Per3) is related to multiple phenotypic parameters, including diurnal preference, sleep homeostasis, infection and cancer. The aim of our study was to investigate the effect of this polymorphism in AMI with ST elevation (STEMI). The study subjects (314 patients of Caucasian origin with STEMI, and 332 healthy controls) were genotyped for Per3 VNTR polymorphism using an allele-specific polymerase chain reaction. A gender difference in circadian rhythmicity of pain onset was observed with significant circadian pattern in men. Furthermore, the Per3(5/5) variant carriers were associated with higher levels of interleukin-6, B-type natriuretic peptide and lower vitamin A levels. By using cosinor analysis we observed different circadian distribution patterns of AMI onset at the level of genotype and allelic frequencies. Genotypes with at least one 4-repeat allele (Per3(4/5) and Per3(4/4)) (N = 264) showed remarkable circadian activity in comparison with Per3(5/5) (N = 50), especially in men. No significant differences in genotype and/or allele frequencies of Per3 VNTR polymorphism were observed when comparing STEMI cases and controls. Our results indicate that the Per3 VNTR may contribute to modulation of cardiac functions and interindividual differences in development and progression of myocardial infarction. PMID:24866331

  18. Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture.

    PubMed

    Lunardi, Franciele Osmarini; Chaves, Roberta Nogueira; de Lima, Laritza Ferreira; Araújo, Valdevane Rocha; Brito, Ivina Rocha; Souza, Carlos Eduardo Azevedo; Donato, Mariana Aragão Matos; Peixoto, Christina Alves; Dinnyes, Andras; Campello, Cláudio Cabral; de Figueiredo, José Ricardo; Rodrigues, Ana Paula Ribeiro

    2015-10-01

    The risk of reintroducing malignant cells after ovarian graft into patients following post-cancer treatment is an obstacle for clinical applications (autotransplantation). In this context, in vitro follicle culture would be an alternative to transplantation in order to minimize such risks. Therefore, the aim of this study was to compare the development of secondary follicles after vitrification in isolated form (without stroma) with vitrification in in situ form (within fragments of ovarian tissue). Follicles were first isolated from ovarian fragments from mixed-breed ewes and then vitrified; these comprised the Follicle-Vitrification group (Follicle-Vit), or fragments of ovarian tissue were first vitrified, followed by isolation of the follicles, resulting in the Tissue-Vitrification group (Tissue-Vit). Control and vitrified groups were submitted to in vitro culture (6 days) and follicular morphology, viability, antrum formation, follicle and oocyte diameter, growth rate, ultrastructural characteristics and cell proliferation were evaluated. The percentages of morphologically normal follicles and antrum formation were similar among groups. Follicular viability and oocyte diameter were similar between Follicle-Vit and Tissue-Vit. The follicular diameter and growth rate of Follicle-Vit were similar to the Control, while those of Tissue-Vit were significantly lower compared to the Control. Both vitrified groups had an augmented rate of granulosa cellular proliferation compared to Control. Secondary follicles can be successfully vitrified before or after isolation from the ovarian tissue without impairing their ability to survive and grow during in vitro culture. PMID:25948481

  19. Photometric and spectroscopic detection of the primary transit of the 111-day-period planet HD 80606 b

    E-print Network

    Moutou, Claire; Bouchy, Francois; Eggenberger, Anne; Boisse, Isabelle; Bonfils, Xavier; Gravallon, Didier; Ehrenreich, David; Forveille, Thierry; Delfosse, Xavier; Desort, Morgan; Lagrange, Anne-Marie; Lovis, Christophe; Mayor, Michel; Pepe, Francesco; Perrier, Christian; Pont, Frederic; Queloz, Didier; Santos, Nuno; Segransan, Damien; Udry, Stephane; Vidal-Madjar, Alfred

    2009-01-01

    We report the detection of the primary transit of the extra-solar planet HD 80606 b, thanks to photometric and spectroscopic observations performed at Observatoire de Haute-Provence, simultaneously with the CCD camera at the 120-cm telescope and the SOPHIE spectrograph at the 193-cm telescope. We observed in both datasets the whole egress of the transit and partially its central part, with the same timings. The ingress occurred before sunset and was not observed. The full duration of the transit is between 9.5 and 17.2 hours. The data allows the planetary radius to be measured (Rp = 0.86 +- 0.10 RJup) and other parameters of the system to be refined. Radial velocity measurements show the detection of a prograde Rossiter-McLaughlin effect, and provide a hint for a spin-orbit misalignment. If confirmed, this misalignment would corroborate the hypothesis that HD 80606 b owes its unusual orbital configuration to Kozai migration. HD 80606 b is by far the transiting planet on the longest period detected today. Its ...

  20. Pharmacokinetics of butorphanol delivered with an osmotic pump during a seven-day period in common peafowl (Pavo cristatus).

    PubMed

    Clancy, Meredith M; KuKanich, Butch; Sykes Iv, John M

    2015-12-01

    OBJECTIVE To determine pharmacokinetics of butorphanol delivered via osmotic pumps in common peafowl (Pavo cristatus) as a method for analgesic administration to avian species. ANIMALS 14 healthy adult male common peafowl. PROCEDURES A preliminary experiment was conducted with 2 birds to establish time point and concentration requirements. Then, the remaining 12 birds were anesthetized, and 2 osmotic pumps containing butorphanol (volume, 2 mL; mean dosage, 247 ?g/kg/h) were implanted subcutaneously in each bird for 7 days prior to removal. Blood samples were collected before pump implantation (time 0); 3, 6, 12, 24, 48, 72, 96, 120, 144, and 168 hours after pump implantation; and 3 and 6 hours after pump removal. Plasma butorphanol concentrations were measured via liquid chromatography-mass spectrometry. RESULTS Plasma concentrations peaked (mean, 106.4 ?g/L; range, 61.8 to 133.0 ?g/L) at a mean of 39.0 hours, with no evidence of sedation in any bird. After pump removal, butorphanol was rapidly eliminated (half-life, 1.45 hours; range, 1.31 to 1.64 hours; n = 5). Mean clearance per fraction of dose absorbed was 2.89 L/kg/h (range, 2.00 to 5.55 L/kg/h). Mean amount of time the plasma butorphanol concentration was ? 60 ?g/L was 85.6 hours (range, 3.5 to 155.3 hours). CONCLUSIONS AND CLINICAL RELEVANCE Plasma concentrations of butorphanol in common peafowl were maintained at or above reported efficacious analgesic concentrations. This study established a method for administering analgesics to avian patients without the need for frequent handling or injections. Use of these osmotic pumps may provide options for avian analgesia. PMID:26618732

  1. Socio-cultural factors surrounding mental distress during the perinatal period in Zambia: a qualitative investigation

    PubMed Central

    2012-01-01

    Background The presence of mental distress during pregnancy and after childbirth imposes detrimental developmental and health consequences for families in all nations. In Zambia, the Ministry of Health (MoH) has proposed a more comprehensive approach towards mental health care, recognizing the importance of the mental health of women during the perinatal period. Aim The study explores factors contributing to mental distress during the perinatal period of motherhood in Zambia. Methods A qualitative study was conducted in Lusaka, Zambia with nineteen focus groups comprising 149 women and men from primary health facilities and schools respectively. Findings There are high levels of mental distress in four domains: worry about HIV status and testing; uncertainty about survival from childbirth; lack of social support; and vulnerability/oppression. Conclusion Identifying mental distress and prompt referral for interventions is critical to improving the mental health of the mother and prevent the effects of mental distress on the baby. Recommendation Strategies should be put in place to ensure pregnant women are screened for possible perinatal mental health problems during their visit to antenatal clinic and referral made to qualified mental health professionals. In addition further research is recommended in order to facilitate evidence based mental health policy formulation and implementation in Zambia. PMID:22954173

  2. Culture.

    ERIC Educational Resources Information Center

    1997

    Twelve conference papers on cultural aspects of second language instruction include: "Towards True Multiculturalism: Ideas for Teachers" (Brian McVeigh); Comparing Cultures Through Critical Thinking: Development and Interpretations of Meaningful Observations" (Laurel D. Kamada); "Authority and Individualism in Japan and the USA" (Alisa Woodring);…

  3. Outdoor Day-Care Centres--A Culturalization of Nature: How Do Children Relate to Nature as Educational Practice?

    ERIC Educational Resources Information Center

    Melhuus, E. Cathrine

    2012-01-01

    This article will discuss how children and adults experience a certain outdoor environment as part of an educational practice, through the activities the adults and children have. It will further discuss how these activities realize cultural values through the educators' and children's activities. In Norway the use of outdoor environments has…

  4. [Retrospective Analysis of the Afatinib Clinical Pathway during the 28-Day Introductory Period-The Japanese Style of Collaborative Drug Therapy Management(J-CDTM)].

    PubMed

    Iwata, Kaori; Ryota, Noriko; Hikita, Ami; Sando, Masumi; Suzuki, Hidekazu; Tamiya, Motohiro; Azuma, Yuichiro; Tani, Eriko; Hamaguchi, Masanari; Tanaka, Ayako; Shiroyama, Takayuki; Morishita, Naoko; Okamoto, Norio; Futagami, Sumiko; Hirashima, Tomonori

    2015-08-01

    Afatinib is a newly approved second-generation epidermal growth factor receptor-tyrosine kinase inhibito r(EGFR-TKI). Afatinib has been shown to prolongthe overall survival of patients with non-small cell lungcancer (NSCLC) with EGFR mutations compared with the standard chemotherapy. However, Grade 3 or 4 toxicities, includingdiarrhea, rash, paronychia, and stomatitis, have been observed more frequently in patients treated with afatinib than in those treated with first-generation EGFR-TKIs. Accordingly, our institution developed an afatinib clinical pathway (the afatinib pathway), which was designed by certified nurses, medical physicians, and certified pharmacists, with the goal of reducing the severity of diarrhea and rash that occur most frequently duringthe 28-day introductory period of afatinib treatment. Between May and October 2014, afatinib was administered accordingto the afatinib pathway to 14 patients with NSCLC and EGFR mutations. Of these patients, only one (7.1%) experienced Grade 3 diarrhea. No other patient experienced Grade 3 or 4 toxicity. The afatinib pathway was effective in reducingthe severities of the diarrhea and rash duringthe 28-day introductory period of the afatinib treatment. Our implementation of the afatinib pathway could be considered the Japanese style of collaborative drugtherapy management (J-CDTM). PMID:26321711

  5. Response of the equatorial topside ionosphere to 27-day variations in solar EUV input during a low solar activity period using C/NOFS

    NASA Astrophysics Data System (ADS)

    Coley, W. R.; Heelis, R. A.

    2012-03-01

    The ion velocity meter (IVM), a part of the CINDI instrument on board the C/NOFS spacecraft, is used to make ion density (Ni) and temperature (Ti) measurements in the low-latitude topside ionosphere in the 400-850 km altitude range during the low-solar activity period 2009-2010. Averages of the Ni measurements are then compared to the daily F10.7 values and the Mg II index values that serve as proxies for solar EUV input to the ionosphere. Before this comparison the IVM ion densities are normalized to a constant 550 km altitude level using the International Reference Ionosphere. Results from daytime data during December 2009-March 2010 indicate the presence of 30-40% Ni variations with an approximately 27-day period. Cross correlation between the density measurements and the daily averaged EUV proxies shows that the ion density variations lag solar EUV by approximately 1 to 3 days in phase. We also note that there is low correlation of the EUV proxies with ion temperature.

  6. Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture.

    PubMed

    Rocha-Frigoni, Nathália Alves de Souza; Leão, Beatriz Caetano da Silva; Nogueira, Ériklis; Accorsi, Mônica Ferreira; Mingoti, Gisele Zoccal

    2015-04-01

    This study examined the effects of antioxidant supplementation and O2 tension on embryo development, cryotolerance and intracellular reactive oxygen species (ROS) levels. The antioxidant supplementation consisted of 0.6 mM cysteine (CYST); 0.6 mM cysteine + 100 ?M cysteamine (C+C); 100 IU catalase (CAT) or 100 ?M ?-mercaptoethanol (?-ME) for 3 or 7 days of in vitro culture (IVC). Two O2 tensions (20% O2 [5% CO2 in air] or 7% O2, 5% CO2 and 88% N2 [gaseous mixture]) were examined. After 7 days of antioxidant supplementation, the blastocyst frequencies were adversely affected (P < 0.05) by CYST (11.2%) and C+C (1.44%), as well as by low O2 tension (17.2% and 11.11% for 20% and 7% O2, respectively) compared with the control (26.6%). The blastocyst re-expansion rates were not affected (P > 0.05) by the treatments (range, 66-100%). After 3 days of antioxidant supplementation, the blastocyst frequencies were not affected (P > 0.05) by any of the antioxidants (range, 43.6-48.5%), but they were reduced by low O2 tension (P < 0.05) (52.1% and 38.4% for 20% and 7% O2, respectively). The intracellular ROS levels, demonstrated as arbitrary fluorescence units, were not affected (P > 0.05) by antioxidant treatment (range, 0.78 to 0.95) or by O2 tension (0.86 and 0.88 for 20% and 7% O2, respectively). The re-expansion rates were not affected (P > 0.05) by any of the treatments (range, 63.6-93.3%). In conclusion, intracellular antioxidant supplementation and low O2 tension throughout the entire IVC period were deleterious to embryo development. However, antioxidant supplementation up to day 3 of IVC did not affect the blastocyst frequencies or intracellular ROS levels. PMID:24040954

  7. Turbulence Kinetic Energy budget during the afternoon transition - Part 1: Observed surface TKE budget and boundary layer description for 10 intensive observation period days

    NASA Astrophysics Data System (ADS)

    Nilsson, E.; Lohou, F.; Lothon, M.; Pardyjak, E.; Mahrt, L.; Darbieu, C.

    2015-11-01

    The decay of turbulence kinetic energy (TKE) and its budget in the afternoon period from mid-day until zero buoyancy flux at the surface is studied in a two-part paper by means of measurements from the Boundary Layer Late Afternoon and Sunset Turbulence (BLLAST) field campaign for 10 Intensive Observation Period days. Here, in Part 1, near-surface measurements from a small tower are used to estimate a TKE budget. The overall boundary layer characteristics and meso-scale situation at the site are also described based upon taller tower measurements, radiosoundings and remote sensing instrumentation. Analysis of the TKE budget during the afternoon transition reveals a variety of different surface layer dynamics in terms of TKE and TKE decay. This is largely attributed to variations in the 8 m wind speed, which is responsible for different amounts of near-surface shear production on different afternoons and variations within some of the afternoon periods. The partitioning of near surface production into local dissipation and transport in neutral and unstably stratified conditions was investigated. Although variations exist both between and within afternoons, as a rule of thumb, our results suggest that about 50 % of the near surface production of TKE is compensated by local dissipation near the surface, leaving about 50 % available for transport. This result indicates that it is important to also consider TKE transport as a factor influencing the near-surface TKE decay rate, which in many earlier studies has mainly been linked with the production terms of TKE by buoyancy and wind shear. We also conclude that the TKE tendency is smaller than the other budget terms, indicating a quasi-stationary evolution of TKE in the afternoon transition. Even though the TKE tendency was observed to be small, a strong correlation to mean buoyancy production of -0.69 was found for the afternoon period. For comparison with previous results, the TKE budget terms are normalized with friction velocity and measurement height and discussed in the framework of Monin-Obukhov similarity theory. Empirically fitted expressions are presented. Alternatively, we also suggest a non-local parametrization of dissipation using a TKE-length scale model which takes into account the boundary layer depth in addition to distance above the ground. The non-local formulation is shown to give a better description of dissipation compared to a local parametrization.

  8. The Presidents' Day cyclone of 18-19 February 1979 - Synoptic overview and analysis of the subtropical jet streak influencing the pre-cyclogenetic period

    NASA Technical Reports Server (NTRS)

    Uccellini, L. W.; Kocin, P. J.; Petersen, R. A.; Wash, C. H.; Brill, K. F.

    1984-01-01

    A detailed examination is undertaken of the subtropical jet (STJ) streak revealed by an analysis of the Presidents' Day cyclone of February 18-19, 1979. During its 24 h period, the STJ's flow became increasingly supergeostrophic and apparently unbalanced, while ageostrophic wind speeds increased to more than 30 m/sec in association with a significant cross-contour component, directed toward lower values of the Montgomery streamfunction, as the flow along the STJ became more divergent with time. These phenomena are linked to the increasing confluence in the entrance region of the jet streak and the decreasing wavelength of the trough-ridge system in which the jet streak was embedded. The upper level divergence and upward vertical motion near the axis of the STJ are found to be important factors in the development of the cyclone's first area of heavy snow.

  9. Practical Physical and Behavioral Measures to Assess the Socialization Spectrum of Cats in a Shelter-Like Setting during a Three Day Period

    PubMed Central

    Slater, Margaret; Garrison, Laurie; Miller, Katherine; Weiss, Emily; Makolinski, Kathleen; Drain, Natasha; Mirontshuk, Alex

    2013-01-01

    Simple Summary Animal welfare organizations accept large numbers of cats with no known history. Because shelters are often highly stressful environments for cats, it is likely to be difficult to differentiate a frightened cat that is socialized to humans from a feral cat that is not. However, this distinction can help channel cats into appropriate dispositions. We conducted structured assessments to measure various behaviors and their potential to distinguish socialization levels. Our results show that a specific set of behaviors are only exhibited by more socialized cats. Many cats needed time to adjust to the shelter-type setting to show these socialized behaviors. Abstract Animal welfare organizations routinely accept large numbers of cats with unknown histories, and whose backgrounds vary from well-socialized pets to cats that have had little or no contact with humans. Agencies are challenged with making the determination of socialization level in a highly stressful environment where cats are often too frightened to show typical behaviors. A variety of structured behavioral assessments were conducted in a shelter-like environment, from intake through a three day holding period, on cats from the full range of socialization as reported by their caregivers. Our results show that certain behaviors such as rubbing, playing, chirping, having the tail up or being at the front of the cage were found to be unique to More Socialized cats. While not all more socialized cats showed these behaviors, cats that did were socialized. Assessing the cats throughout the three day period was beneficial in eliciting key behaviors from shyer and more frightened cats. These results will be used in future work to develop an assessment tool to identify the socialization status of cats as a standardized guide for transparent and reliable disposition decisions and higher live release rates for cats in animal shelters. PMID:26479757

  10. The analysis of fundamental period of cultural heritage buildings: experimental data for church towers in Basilicata (Southern Italy)

    NASA Astrophysics Data System (ADS)

    Gizzi, Fabrizio T.; Liberatore, Domenico; Masini, Nicola; Sileo, Maria; Zotta, Cinzia; Potenza, Maria Rosaria; Scavone, Manuela; Sorrentino, Luigi

    2014-05-01

    Seismic hazard is among the main factors conditioning the conservation of historical centres and cultural heritage located in them. This consideration is suitable especially for downtown areas located in Italy, whose territory is prone to seismic hazards, in the southern area especially. As a matter of fact, the historical sources inform us that most of monuments located in Southern Italy suffered damage and consequent restoration or rebuilding due to the earthquake of the past. Therefore, knowing what buildings are the most exposed to the seismic risk can help the stakeholders to fix priority actions aimed at mitigating the effects of future events. Starting from these preliminary remarks, in the framework of the Project PRO_CULT, we started an extensive campaign of measurements of dynamic features of the church towers in some towns of the Basilicata Region (Southern Italy). The aim of the research activity is to assess the fundamental period of such a typology of historical buildings and comparing it with the dynamic features of the foundation soil to put into evidence possible resonance phenomena responsible of an increase of building damage during the seismic shaking. The selection of the towns to be considered as a target of the experimental survey was performed taking into account the availability of written sources dealing with the historical seismic effects suffered by the bell-towers over the centuries with special attention to the sites heavily affected by the 16 December 1857 Basilicata and 23 November 1980 Irpinia-Basilicata earthquakes (Gizzi and Masini 2007). The fundamental period of bell-towers is estimated using ambient noise vibration signals recorded at the highest level of the towers. The techniques used to get the dynamic values are both the Horizontal to Vertical Spectral Ratio (HVSR) and the Horizontal to Horizontal Spectral Ratio (HHSR) (Liberatore et al. 2008). Once the fundamental frequency has been estimated, it is compared with the dynamic features of the soil obtained by free-field measurements. The detailed architectural survey of each investigated tower will also allow correlating the estimated frequencies with the features of the historic building such as structural symmetry, number of storeys and height of the tower, building materials, presence and dimension of openings, presence of tie rods, and presence of adjoining buildings. In this way, we can get new insights for a tentative scheme of typological classification of such historic buildings. References Gizzi F.T., Masini N. 2007. Historical earthquakes and damage patterns in Potenza (Basilicata, southern Italy), Annals of Geophysics, 50 (5), pp.675-687, doi: 10.4401/ag-3061 Liberatore D., Mucciarelli M., Gallipoli M. R., Masini N. 2008, Two Applications of the HVSR Technique to Cultural Heritage and Historical Masonry in Increasing Seismic Safety by Combining Engineering Technologies and Seismological Data, M. Mucciarelli, M. Herak and J. Cassidy eds., Springer, [ISBN 978-1-4020-9196-4; ISSN: 1871-4668], 325-336, doi: 10.1007/978-1-4020-9196-4_22 Acknowledgements The authors thanks Basilicata Region for supporting this activity in the framework of the Project "PRO_CULT" (Advanced methodological approaches and technologies for Protection and Security of Cultural Heritage ) financed by Regional Operational Programme ERDF 2007/2013

  11. A Whole-Genome Microarray Study of Arabidopsis thaliana Semisolid Callus Cultures Exposed to Microgravity and Nonmicrogravity Related Spaceflight Conditions for 5 Days on Board of Shenzhou 8

    PubMed Central

    Neef, Maren; Ecke, Margret; Hampp, Rüdiger

    2015-01-01

    The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1?g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production. PMID:25654111

  12. A whole-genome microarray study of Arabidopsis thaliana semisolid callus cultures exposed to microgravity and nonmicrogravity related spaceflight conditions for 5 days on board of Shenzhou 8.

    PubMed

    Fengler, Svenja; Spirer, Ina; Neef, Maren; Ecke, Margret; Nieselt, Kay; Hampp, Rüdiger

    2015-01-01

    The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1?g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production. PMID:25654111

  13. 28-day oral safety evaluation of extracellular polysaccharopeptides produced in submerged culture from the turkey tail medicinal mushroom Trametes versicolor (L.:Fr.) Pilát LH-1 in mice.

    PubMed

    Lai, Chun-Hong; Teng, Ju-Fang; Hsu, Tai-Hao; Lin, Fang-Yi; Yang, Po-Wen; Lo, Hui-chen

    2011-01-01

    Turkey tail medicinal mushroom, Trametes versicolor (TV), is a species with a variety of pharmacological activities. Its intracellular polysaccharopeptides are widely commercialized. Recently, we found a novel TV strain LH-1 in Taiwan and demonstrated that the extracellular polysaccharopeptide (ePSP) of LH-1 obtained from submerged culture exhibits significant immunomodulatory activity. In this in vivo study, we further evaluated the safety of orally administered LH-1 ePSP using both male and female ICR mice. The LH-1 ePSP was orally administered to mice at levels of 0 (water), 100 (low dose), 500 (medium dose), or 1000 mg/kg/day (high dose) for 28 days. Clinical observations, growth, food consumption, histopathological examination, and clinical biochemical analyses revealed no adverse effects of LH-1 ePSP in mice. There were no significant differences in the results of target organ weights, hematological analyses, and urinalysis examination among groups. However, male mice that ingested high doses of LH-1 ePSP tended to have decreased lung weights and platelet numbers. In conclusion, the results of the present study suggested that oral administration of LH-1 ePSP for 28 days is accompanied by no obvious signs of toxicity. The lack of toxicity supports the potential use of LH-1 ePSP as a food or dietary supplement. PMID:22135874

  14. Ground-based observations of Saturn's auroral ionosphere over three days: Trends in H3+ temperature, density and emission with Saturn local time and planetary period oscillation

    NASA Astrophysics Data System (ADS)

    O'Donoghue, James; Melin, Henrik; Stallard, Tom S.; Provan, G.; Moore, Luke; Badman, Sarah V.; Cowley, Stan W. H.; Baines, Kevin H.; Miller, Steve; Blake, James S. D.

    2016-01-01

    On 19-21 April 2013, the ground-based 10-m W.M. Keck II telescope was used to simultaneously measure H3+ emissions from four regions of Saturn's auroral ionosphere: (1) the northern noon region of the main auroral oval; (2) the northern midnight main oval; (3) the northern polar cap and (4) the southern noon main oval. The H3+ emission from these regions was captured in the form of high resolution spectral images as the planet rotated. The results herein contain twenty-three H3+ temperatures, column densities and total emissions located in the aforementioned regions - ninety-two data points in total, spread over timescales of both hours and days. Thermospheric temperatures in the spring-time northern main oval are found to be cooler than their autumn-time southern counterparts by tens of K, consistent with the hypothesis that the total thermospheric heating rate is inversely proportional to magnetic field strength. The main oval H3+ density and emission is lower at northern midnight than it is at noon, in agreement with a nearby peak in the electron influx in the post-dawn sector and a minimum flux at midnight. Finally, when arranging the northern main oval H3+ parameters as a function of the oscillation period seen in Saturn's magnetic field - the planetary period oscillation (PPO) phase - we see a large peak in H3+ density and emission at ?115° northern phase, with a full-width at half-maximum (FWHM) of ?44°. This seems to indicate that the influx of electrons associated with the PPO phase at 90° is responsible at least in part for the behavior of all H3+ parameters. A combination of the H3+ production and loss timescales and the ±10° uncertainty in the location of a given PPO phase are likely, at least in part, to be responsible for the observed peaks in H3+ density and emission occurring at a later time than the peak precipitation expected at 90° PPO phase.

  15. Day to day with COPD

    MedlinePLUS

    ... but there are things you can do every day to keep COPD from getting worse, to protect ... COPD - day to day; Chronic obstructive airways disease - day to day; Chronic obstructive lung disease - day to day ; Chronic ...

  16. KELT-6b: A P ? 7.9 day hot Saturn transiting a metal-poor star with a long-period companion

    SciTech Connect

    Collins, Karen A.; Kielkopf, John F.; Eastman, Jason D.; Beatty, Thomas G.; Gaudi, B. Scott; Siverd, Robert J.; Pepper, Joshua; Stassun, Keivan G.; Johnson, John Asher; Howard, Andrew W.; Fulton, Benjamin J.; Fischer, Debra A.; Manner, Mark; Bieryla, Allyson; Latham, David W.; Gregorio, Joao; Buchhave, Lars A.; Jensen, Eric L. N.; Penev, Kaloyan; Crepp, Justin R.; and others

    2014-02-01

    We report the discovery of KELT-6b, a mildly inflated Saturn-mass planet transiting a metal-poor host. The initial transit signal was identified in KELT-North survey data, and the planetary nature of the occulter was established using a combination of follow-up photometry, high-resolution imaging, high-resolution spectroscopy, and precise radial velocity measurements. The fiducial model from a global analysis including constraints from isochrones indicates that the V = 10.38 host star (BD+31 2447) is a mildly evolved, late-F star with T {sub eff} = 6102 ± 43 K, log?g{sub ?}=4.07{sub ?0.07}{sup +0.04}, and [Fe/H] = –0.28 ± 0.04, with an inferred mass M {sub *} = 1.09 ± 0.04 M {sub ?} and radius R{sub ?}=1.58{sub ?0.09}{sup +0.16} R{sub ?}. The planetary companion has mass M{sub P} = 0.43 ± 0.05 M {sub Jup}, radius R{sub P}=1.19{sub ?0.08}{sup +0.13} R{sub Jup}, surface gravity log?g{sub P}=2.86{sub ?0.08}{sup +0.06}, and density ?{sub P}=0.31{sub ?0.08}{sup +0.07} g cm{sup ?3}. The planet is on an orbit with semimajor axis a = 0.079 ± 0.001 AU and eccentricity e=0.22{sub ?0.10}{sup +0.12}, which is roughly consistent with circular, and has ephemeris of T {sub c}(BJD{sub TDB}) = 2456347.79679 ± 0.00036 and P = 7.845631 ± 0.000046 days. Equally plausible fits that employ empirical constraints on the host-star parameters rather than isochrones yield a larger planet mass and radius by ?4)-7). KELT-6b has surface gravity and incident flux similar to HD 209458b, but orbits a host that is more metal poor than HD 209458 by ?0.3 dex. Thus, the KELT-6 system offers an opportunity to perform a comparative measurement of two similar planets in similar environments around stars of very different metallicities. The precise radial velocity data also reveal an acceleration indicative of a longer-period third body in the system, although the companion is not detected in Keck adaptive optics images.

  17. HAT-P-24b: An Inflated Hot Jupiter on a 3.36 Day Period Transiting a Hot, Metal-poor Star

    NASA Astrophysics Data System (ADS)

    Kipping, D. M.; Bakos, G. Á.; Hartman, J.; Torres, G.; Shporer, A.; Latham, D. W.; Kovács, Géza; Noyes, R. W.; Howard, A. W.; Fischer, D. A.; Johnson, J. A.; Marcy, G. W.; Béky, B.; Perumpilly, G.; Esquerdo, G. A.; Sasselov, D. D.; Stefanik, R. P.; Lázár, J.; Papp, I.; Sári, P.

    2010-12-01

    We report the discovery of HAT-P-24b, a transiting extrasolar planet orbiting the moderately bright V = 11.818 F8 dwarf star GSC 0774-01441, with a period P = 3.3552464 ± 0.0000071 days, transit epoch Tc = 2455216.97669 ± 0.00024 (BJD)11, and transit duration 3.653 ± 0.025 hr. The host star has a mass of 1.191 ± 0.042 M sun, radius of 1.317 ± 0.068 R sun, effective temperature 6373 ± 80 K, and a low metallicity of [Fe/H] = -0.16 ± 0.08. The planetary companion has a mass of 0.681 ± 0.031 M J and radius of 1.243 ± 0.072 R J yielding a mean density of 0.439 ± 0.069 g cm-3. By repeating our global fits with different parameter sets, we have performed a critical investigation of the fitting techniques used for previous Hungarian-made Automated Telescope planetary discoveries. We find that the system properties are robust against the choice of priors. The effects of fixed versus fitted limb darkening are also examined. HAT-P-24b probably maintains a small eccentricity of e = 0.052+0.022 -0.017, which is accepted over the circular orbit model with false alarm probability 5.8%. In the absence of eccentricity pumping, this result suggests that HAT-P-24b experiences less tidal dissipation than Jupiter. Due to relatively rapid stellar rotation, we estimate that HAT-P-24b should exhibit one of the largest known Rossiter-McLaughlin effect amplitudes for an exoplanet (?V RM ~= 95 m s-1) and thus a precise measurement of the sky-projected spin-orbit alignment should be possible. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NOAO and NASA.

  18. KELT-6b: A P ~ 7.9 Day Hot Saturn Transiting a Metal-poor Star with a Long-period Companion

    NASA Astrophysics Data System (ADS)

    Collins, Karen A.; Eastman, Jason D.; Beatty, Thomas G.; Siverd, Robert J.; Gaudi, B. Scott; Pepper, Joshua; Kielkopf, John F.; Johnson, John Asher; Howard, Andrew W.; Fischer, Debra A.; Manner, Mark; Bieryla, Allyson; Latham, David W.; Fulton, Benjamin J.; Gregorio, Joao; Buchhave, Lars A.; Jensen, Eric L. N.; Stassun, Keivan G.; Penev, Kaloyan; Crepp, Justin R.; Hinkley, Sasha; Street, Rachel A.; Cargile, Phillip; Mack, Claude E.; Oberst, Thomas E.; Avril, Ryan L.; Mellon, Samuel N.; McLeod, Kim K.; Penny, Matthew T.; Stefanik, Robert P.; Berlind, Perry; Calkins, Michael L.; Mao, Qingqing; Richert, Alexander J. W.; DePoy, Darren L.; Esquerdo, Gilbert A.; Gould, Andrew; Marshall, Jennifer L.; Oelkers, Ryan J.; Pogge, Richard W.; Trueblood, Mark; Trueblood, Patricia

    2014-02-01

    We report the discovery of KELT-6b, a mildly inflated Saturn-mass planet transiting a metal-poor host. The initial transit signal was identified in KELT-North survey data, and the planetary nature of the occulter was established using a combination of follow-up photometry, high-resolution imaging, high-resolution spectroscopy, and precise radial velocity measurements. The fiducial model from a global analysis including constraints from isochrones indicates that the V = 10.38 host star (BD+31 2447) is a mildly evolved, late-F star with T eff = 6102 ± 43 K, log g_\\star =4.07_{-0.07}^{+0.04}, and [Fe/H] = -0.28 ± 0.04, with an inferred mass M sstarf = 1.09 ± 0.04 M ? and radius R_\\star =1.58_{-0.09}^{+0.16} \\,R_\\odot. The planetary companion has mass MP = 0.43 ± 0.05 M Jup, radius R_{P}=1.19_{-0.08}^{+0.13} \\,R_Jup, surface gravity log g_{P}=2.86_{-0.08}^{+0.06}, and density \\rho _{P}=0.31_{-0.08}^{+0.07}\\,g\\,cm^{-3}. The planet is on an orbit with semimajor axis a = 0.079 ± 0.001 AU and eccentricity e=0.22_{-0.10}^{+0.12}, which is roughly consistent with circular, and has ephemeris of T c(BJDTDB) = 2456347.79679 ± 0.00036 and P = 7.845631 ± 0.000046 days. Equally plausible fits that employ empirical constraints on the host-star parameters rather than isochrones yield a larger planet mass and radius by ~4}-7}. KELT-6b has surface gravity and incident flux similar to HD 209458b, but orbits a host that is more metal poor than HD 209458 by ~0.3 dex. Thus, the KELT-6 system offers an opportunity to perform a comparative measurement of two similar planets in similar environments around stars of very different metallicities. The precise radial velocity data also reveal an acceleration indicative of a longer-period third body in the system, although the companion is not detected in Keck adaptive optics images. KELT is a joint project of The Ohio State University, Vanderbilt University, and Lehigh University.

  19. Day to Day

    ERIC Educational Resources Information Center

    Jurecki, Dennis

    2006-01-01

    A clean, healthy and safe school provides students, faculty and staff with an environment conducive to learning and working. However, budget and staff reductions can lead to substandard cleaning practices and unsanitary conditions. Some school facility managers have been making the switch to a day-schedule to reduce security and energy costs, and…

  20. Parathyroid hormone blocks the stimulatory effect of insulin-like growth factor-I on collagen synthesis in cultured 21-day fetal rat calvariae

    SciTech Connect

    Kream, B.E.; Petersen, D.N.; Raisz, L.G. )

    1990-01-01

    We examined the interaction of parathyroid hormone (PTH) and recombinant human insulin-like growth factor I (IGF-I) on collagen synthesis in 21-day fetal rat calvariae as assessed by measuring the incorporation of ({sup 3}H)proline into collagenase-digestible protein. After 96 hours of culture, 10 nM PTH antagonized the stimulation of collagen synthesis and partially blocked the increase in dry weight produced by 10 nM IGF-I. The effect of PTH to block IGF-I stimulated collagen synthesis was observed in the central bone of calvariae and was mimicked by forskolin and phorbol 12-myristate 13-acetate, but not by 1,25-dihydroxyvitamin D3, transforming growth factor-alpha or dexamethasone. Our data are consistent with the concept that the direct effect of PTH is to inhibit basal CDP labeling and fully oppose IGF-I stimulated CDP labeling. The finding that this effect of PTH is mimicked by forskolin and PMA suggests that this block in IGF-I stimulation of CDP labeling involves both cAMP and protein kinase C mediated pathways.

  1. Investigation of near-infrared spectroscopy for periodic in situ cell culture media determination of glucose and lactate 

    E-print Network

    Lewis, Christopher Bryan

    1999-01-01

    and to build a successful multivariate calibration model. Calibration, using a combination of spectra from cell culture media and prepared media mixtures, was performed and applied to an independent validation set, resulting in a prediction error of 14.8 mg...

  2. Producing biodiesel from cotton seed oil using Rhizopus oryzae ATTC #34612 whole cell biocatalysts: Culture media and cultivation period optimization

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effect of culture medium composition and cultivation time on biodiesel production by Rhizopus oryzae ATCC #34612 whole cell catalysts, immobilized on novel rigid polyethylene biomass supports, was investigated. Supplementation of the medium with carbon sources led to higher lipase activity and i...

  3. 25 CFR 291.9 - What must the Secretary do at the end of the 60-day comment period if the State offers an...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...Class III gaming procedures? Within 30 days of receiving the State's alternative proposal, the Secretary must appoint a mediator who: (a) Has no official, financial, or personal conflict of interest with respect to the issues in controversy;...

  4. Effects of timing on vaccination (day 0 versus day 14 of a receiving period) with a modified-live respiratory viral vaccine on performance, feed intake, and febrile response of beef heifers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this study was to evaluate the effects of timing of administration of a modified-live respiratory viral vaccine (IBR-PI3-BRSV-BVD) on d 0 or on d 14 of a receiving period on performance, feed intake, and febrile response in beef heifers. Our hypothesis was vaccine timing will alter ...

  5. Increase in nitrate uptake by soybean plants during interruption of the dark period with low intensity light

    NASA Technical Reports Server (NTRS)

    Raper, C. D. Jr; Vessey, J. K.; Henry, L. T.

    1991-01-01

    Diurnal patterns of net NO3- uptake by nonnodulated soybean [Glycine max (L.) Merr. cv. Ransom] plants growing in flowing hydroponic culture at 26 and 16 degrees C root temperatures were measured at hourly intervals during alternate days of a 12-day growth period. Ion chromatography was used to determine removal of NO3- from the culture solution. Day and night periods of 9 and 15 h were used during growth. The night period included two 6-h dark periods and an intervening 3-h period of night interruption by incandescent lamps to effect a long-day photoperiod and repress floral initiation. At both root temperatures, the average specific rates of NO3- uptake were twice as great during the night interruption period as during the day period; they were greater during the day period than during the dark periods; and they were greater during the dark period immediately following the day period than during the later dark period that followed the night interruption. While these average patterns were repetitious among days, measured rates of uptake varied hourly and included intervals of net efflux scattered through the day period and more frequently through the 2 dark periods. Root temperature did not affect the average daily specific rates of uptake or the qualitative relationships among day, dark and night interruption periods of the diurnal cycle.

  6. [Dynamics and functions of exometabolites in the process of the growth of Escherichia coli M-17 periodical culture].

    PubMed

    Vakhitov, T Ia; Momot, E N; Tolparov, Iu N

    2005-01-01

    The concentrations of metabolites altered immediately after culture inoculation into fresh medium and depended on the their dilution (in small dilutions metabolites were released into the medium, and, on the contrary, in great dilutions they were adsorbed). Acetate was the main exometabolite. The growth of culture started only when the acetate concentration reached the threshold value. At the stage of growth deceleration an intensive release of acetate and other exometabolites occurred. The process had the explosive character of chemical chain reaction. It could be initiated by high local concentrations of metabolites around bacteria, most sensitive to changes in growth conditions. At a high concentration of such cells the signal could be transmitted from one bacteria to another, otherwise the reaction chain was quickly broken and no mass release of metabolites occurred. At the stationary phase succinate, valine and lactate were consumed by bacteria with lactate concentration dropping practically to 0. At the same time acetate and citrate concentrations oscillated about some constant value. The release of growth inhibitors, formiate and alanine, continued to the end of cultivation, which was, seemingly, one of the factors of growth cessation. The specific features of the dymamics of metabolites were indicative of the fact that they had their individual regulatory functions. The problem of the self-synchronization of the bacterial culture is discussed. PMID:15773393

  7. DNA damage in mononuclear leukocytes of farmers measured using the alkaline comet assay: modifications of DNA damage levels after a one-day field spraying period with selected pesticides.

    PubMed

    Lebailly, P; Vigreux, C; Lechevrel, C; Ledemeney, D; Godard, T; Sichel, F; LeTalaër, J Y; Henry-Amar, M; Gauduchon, P

    1998-10-01

    The alkaline comet assay was used to assess DNA damage in mononuclear leukocytes of farmers before and after a 1-day spraying period with selected pesticides under usual conditions. Two blood samples were collected, one in the morning of the day of spraying (S0) and the second in the morning of the day after (S1). Here, we assessed variations in DNA damage levels between these two sampling times. Four groups of farmers were formed, according to exposure to: (a) various fungicide-insecticide mixtures (including chlorothalonil; group 1, n = 8), (b) the herbicide isoproturon (group 2, n = 11), (c) fungicide triazoles (group 3, n = 14), and (d) a fungicide (chlorothalonil)-insecticide mixture (group 4, n = 8). An increase in DNA damage levels was observed at S1 for groups 1 and 4, who were exposed to similar pesticides. This increase was correlated with area sprayed between S0 and S1 and with the number of spraying tanks used over this 1-day period. No effect was observed on cell viability or on hematological parameters for these two groups. No statistically significant modification of DNA damage level was observed the day after spraying for groups 2 and 3, when each was observed as a whole. However, some farmers presented significantly more DNA damage after exposure, and others presented less damage. In these two groups, a significant decrease of neutrophils was observed at S1, and a decrease of red blood cells was observed in group 3. In parallel, a significant loss of lymphocyte viability was observed in these two groups. A 1-day spraying period seems to be sufficient to significantly modify DNA damage levels in mononuclear leukocytes, but the correlation of this change with pesticide-related exposure parameters depends on the kind of pesticide concerned. PMID:9796639

  8. Radiometric detection of yeasts in blood cultures of cancer patients

    SciTech Connect

    Hopfer, R.L.; Orengo, A.; Chesnut, S.; Wenglar, M.

    1980-09-01

    During a 12-month period, 19,457 blood cultures were collected. Yeasts were isolated from 193 cultures derived from 76 cancer patients. Candida albicans or Candida tropicalis accounted for 79% of isolates. Of the three methods compared, the radiometric method required 2.9 days to become positive, blind subculture required 2.6 days, and Gram stains required 1 day. However, the radiometric method was clearly superior in detecting positive cultures, since 73% of all cultures were first detected radiometrically, 22% were detected by subculture, and only 5% were detected by Gram stain. Although 93% of the isolates were detected by aerobic culture, five (7%) isolates were obtained only from anaerobic cultures. Seven days of incubation appear to be sufficient for the radiometric detection of yeasts.

  9. CONTENT KNOWLEDGE (Declarative Knowledge): Students will demonstrate knowledge of Jewish history, culture and thought Biblical times to the present day. They will

    E-print Network

    Fernandez, Eduardo

    texts, Jewish history in Temple times, Judaism and the foundations of Christianity and Islam; Medieval Jewish history, the changing role of women in Jewish culture, Hasidism, and the Jews of Eastern Europe

  10. Japan: The Modernization of an Ancient Culture. Series on Public Issues No. 3.

    ERIC Educational Resources Information Center

    Wolken, Lawrence C.

    This booklet, one of a series of booklets intended to apply economic principles to major social and political issues of the day, traces the modernization of the ancient culture of Japan. Four major areas are covered: (1) "An Ancient Culture" covers the period from the first settling of Japan through the Heian period, the medieval ages, the Meiji…

  11. No Matter How Long the Night, the Day is Sure to Come: Culture and Educational Transformation in Post-Colonial Namibia and Post-Apartheid South Africa

    NASA Astrophysics Data System (ADS)

    Nekhwevha, Fhulu

    1999-11-01

    Following the defeat of Apartheid, the 1990s have witnessed serious attempts by Namibians and South Africans alike to reconstruct their social institutions along democratic lines. While education has not been excluded from these efforts, there is evidence that the new curricula are primarily influenced by western educational models. For example, prescriptions of the International Monetary Fund and the World Bank have been uncritically incorporated into the new educational programme. Consequently the curricula lack an indigenous ingredient, namely the cultural capital of the African masses. It is suggested in this article that the much acclaimed African cultural renaissance in education will only become a reality when educationalists embrace the "pedagogy of hope".

  12. Final Days

    MedlinePLUS

    ... mouth, fingers, toes What to report to the hospice/palliative care team? ? Changes in the person such ... social worker, chaplain or other members of the hospice team ? Religious, cultural or ethnic traditions important to ...

  13. [All-cause mortality among men of elderly and senile age depending on day of week, a season and changing to daylight saving time for a 13-year period].

    PubMed

    Fedorets, V N; Dul'ski?, V A; Mozerova, E M; Kozlov, K L; Titkov, Iu S

    2012-01-01

    To study the all-cause mortality among men of elderly and senile age depending on day of week, month of year, a season, changing to daylight saving time, prospective supervision over 1067 men of 60 years and upward for a 13-year period was conducted. The all-cause mortality depending on transition into daylight saving time was counted for March and October and compared to indicators of April and November accordingly. The all-cause mortality rate had made 1668,5 on 1000. This indicator in January (177.9 per 1000) and August (175.2 per 1000) was higher, than in November (102.4 per 1000; p < 0.05). Dependence of all-cause mortality rate on day of week, a season, changing to daylight saving time was not revealed. The analysis of all-cause mortality rate on days of week in various seasons has revealed that in autumn the greatest all-cause mortality rate was observed on Sunday (83.6 per 1000), the least--on Monday (35.0 per 1000). Besides, on Monday the greatest all-cause mortality rate was observed in winter (35.5%, or 72.8 per 1000), and the least one--in autumn (17.1%, or 35.0 per 1000). During other moments of supervision statically significant distinctions were not revealed. PMID:23130510

  14. Silicon cell culture templates with nanotopography: periodic nanostructures and random nanoporous topologies generated by high-repetition rate sub-15 fs pulsed near-infrared laser light

    NASA Astrophysics Data System (ADS)

    Straub, Martin; Uchugonova, Aisada; Koch, Marcus; König, Karsten

    2012-03-01

    In recent years a variety of studies has demonstrated that artificially generated microenvironments can exert a strong influence on cell growth, cell adhesion, proliferation, and differentiation behavior in the culture dish. In particular, cells tend to adapt themselves to elongated micro- and nanostructures. Thus, nanostructured substrates are of significant interest in the biological and biomedical sciences as adhesion and development of cells can be controlled via the topological surface properties. In contrast to earlier approaches relying on electron beam or nanoimprint lithography, nanostructures were produced on Si(100) surfaces using sub-15 femtosecond high-resolution laser scanning microscopy. Laser processing was performed with the silicon surface immersed in water followed by hydrofluoric acid etching in order to remove silicon oxide residues. Ripples of at a periodicity of 150 nm as well as random nanoporous surface arrangements were generated by Ti:Sapphire laser light of centre wavelength 800 nm (bandwidth 120 nm, repetition rate 85 MHz) at picojoule pulse energies. Growth of Chinese hamster ovary (CHO) cells revealed good adhesion to the silicon substrates. Importantly, alignment of cells along the direction of ripples was observed, whereas randomly nanoporous surfaces did not induce any preferences in cell orientation.

  15. Dinosaur Day!

    ERIC Educational Resources Information Center

    Nakamura, Sandra; Baptiste, H. Prentice

    2006-01-01

    In this article, the authors describe how they capitalized on their first-grade students' love of dinosaurs by hosting a fun-filled Dinosaur Day in their classroom. On Dinosaur Day, students rotated through four dinosaur-related learning stations that integrated science content with art, language arts, math, and history in a fun and time-efficient…

  16. Day Care.

    ERIC Educational Resources Information Center

    Merro, John; And Others

    Interviews on the quality of day care in the United States are presented in this transcript of a program broadcast in the National Public Radio weekly series, "Options in Education." Writers, day care center personnel and others describe and evaluate the current situation. Federal legislation concerning children is examined, and researchers…

  17. 34 CFR 300.11 - Day; business day; school day.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Day; business day; school day. 300.11 Section 300.11... CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.11 Day; business day; school day. (a) Day means calendar day unless otherwise indicated as business day or school day. (b) Business...

  18. 34 CFR 300.11 - Day; business day; school day.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Day; business day; school day. 300.11 Section 300.11... CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.11 Day; business day; school day. (a) Day means calendar day unless otherwise indicated as business day or school day. (b) Business...

  19. Optimization of Periprosthetic Culture for Diagnosis of Propionibacterium acnes Prosthetic Joint Infection?

    PubMed Central

    Butler-Wu, Susan M.; Burns, Erica M.; Pottinger, Paul S.; Magaret, Amalia S.; Rakeman, Jennifer L.; Matsen, Frederick A.; Cookson, Brad T.

    2011-01-01

    Propionibacterium acnes is increasingly recognized as an important agent of prosthetic joint infection (PJI). However, the optimum culture conditions for recovery of this organism from PJI specimens have not been determined. By applying a prolonged 28-day culture incubation to all periprosthetic specimens received for bacterial culture from 198 revision arthroplasty procedures, we retrospectively determined that a 13-day culture incubation period is necessary for the recovery of P. acnes from patients with PJI. Incubation beyond this period was associated with increasing recovery of nondiagnostic isolates: 21.7% of P. acnes isolates believed to be clinically unimportant were recovered after 13 days of incubation. Importantly, a diagnosis of P. acnes PJI would have been missed in 29.4% of patients had extended culture incubation been applied only to anaerobic culture media. Although specimens from P. acnes PJIs were more commonly associated with the presence of ?2 culture media positive for growth, acute inflammation (?5 neutrophils/high-power field) was observed in only 40% of patients with PJIs that had more than one specimen submitted for bacterial culture. These results support the need for a minimum culture incubation period of 13 days to be applied to both aerobic and anaerobic culture media for all periprosthetic specimens. Optimal recovery of infecting organisms from PJI specimens will be an important component in generating a universal definition for PJI due to indolent agents of infection, such as P. acnes. PMID:21543562

  20. World Population Day in Beijing.

    PubMed

    1993-08-01

    A mass rally sponsored by the State Family Planning Commission (SFPC), the China Family Planning Association, the Population Culture Promotion Association of China, the Beijing Family Planning Committee, and the UNFPA was held July 10, 1993, in Beijing to mark World Population Day. Approximately 1000 people attended the rally including State Councillor and SFPC Minister Peng Peiyun who delivered a speech. She noted that the Chinese government has given significant attention to controlling population growth and that population norms on childbearing and reproductive behaviors have changed over the twenty years of family planning program implementation. The decline in the rate of total fertility over the period from 5-6 to 2 has greatly helped stabilize world population. Peng also stressed the need to continue improving the practice of the family planning program to help improve general economic conditions and individual living standards in the country. Plans exist to continue to strengthen cooperation and exchange with UNFPA and other countries in the effort to learn from other successful experiences in the field. Counseling and information on population and family planning, along with art and literary performances on controlling population growth were conducted as publicity activities during the day in the eight districts of Beijing. An exhibition on sex was also held in Zhongshan Park. PMID:12287602

  1. Inspire Day

    ERIC Educational Resources Information Center

    Bohach, Barbara M.; Meade, Birgitta

    2014-01-01

    The authors collaborated on hosting a "Spring Inspire Day." planned and delivered by preservice elementary teachers as a social studies/science methods project. Projects that have authentic application opportunities can make learning meaningful for prospective teachers as well as elementary students. With the impetus for an integrated…

  2. 75 FR 42818 - Agency Information Collection; Activity Under OMB Review; Collection of Safety Culture Data for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... on March 12, 2010 (75 FR 11988) and the comment period ended on May 11, 2010. The 60-day notice...; Collection of Safety Culture Data for Program Evaluation AGENCY: Research & Innovative Technology... Culture Data for Program Evaluation. Type of Request: Approval of a new information collection....

  3. TRACES OF A LOST LANDSCAPE TRADITION AND CROSS-CULTURAL RELATIONSHIPS BETWEEN KOREA, CHINA AND JAPAN IN THE EARLY JOSEON PERIOD (1392-1550)

    E-print Network

    Lee, Sangnam

    2014-08-31

    This dissertation traces a lost landscape tradition and investigates cross-cultural relationships between Korea, China and Japan during the fifteenth and mid sixteenth centuries. To this end, the main research is given to ...

  4. CoRoT 101186644: A transiting low-mass dense M-dwarf on an eccentric 20.7-day period orbit around a late F-star. Discovered in the CoRoT lightcurves

    NASA Astrophysics Data System (ADS)

    Tal-Or, L.; Mazeh, T.; Alonso, R.; Bouchy, F.; Cabrera, J.; Deeg, H. J.; Deleuil, M.; Faigler, S.; Fridlund, M.; Hébrard, G.; Moutou, C.; Santerne, A.; Tingley, B.

    2013-05-01

    We present the study of the CoRoT transiting planet candidate 101186644, also named LRc01_E1_4780. Analysis of the CoRoT lightcurve and the HARPS spectroscopic follow-up observations of this faint (mV = 16) candidate revealed an eclipsing binary composed of a late F-type primary (Teff = 6090 ± 200 K) and a low-mass, dense late M-dwarf secondary on an eccentric (e = 0.4) orbit with a period of ~20.7 days. The M-dwarf has a mass of 0.096 ± 0.011 M?, and a radius of 0.104-0.006+0.026 R?, which possibly makes it the smallest and densest late M-dwarf reported so far. Unlike the claim that theoretical models predict radii that are 5-15% smaller than measured for low-mass stars, this one seems to have a radius that is consistent and might even be below the radius predicted by theoretical models. Based on observations made with the 1-m telescope at the Wise Observatory, Israel, the Swiss 1.2-m Leonhard Euler telescope at La Silla Observatory, Chile, the IAC-80 telescope at the Observatory del Teide, Canarias, Spain, and the 3.6-m telescope at La Silla Observatory (ESO), Chile (program 184.C-0639).

  5. In vitro maintenance of spermatogenesis in Xenopus laevis testis explants cultured in serum-free media

    SciTech Connect

    Risley, M.S.; Miller, A.; Bumcrot, D.A.

    1987-05-01

    Spermatogenesis has been maintained for extended periods in Xenopus laevis testis explants cultured in serum-free media supplemented with bovine serum albumin, insulin, transferrin, follicle-stimulating hormone, dihydrotestosterone, testosterone, retinol, ascorbate, and tocopherol. The organization of the testis fragments was maintained for 28 days, and all stages of development were present throughout the culture period. /sup 3/H-Thymidine-labeled secondary (Type B) spermatogonia developed in 28 days into spermatids at the acrosomal vesicle stage whereas labeled zygotene spermatocytes became mature spermatids in 28 days. Spermatogonial proliferation also continued in vitro for 28 days. Germ cell differentiation was not dependent upon exogenous testosterone, ascorbate, or tocopherol since /sup 3/H-labeled spermatogonia became mature spermatids in testes cultured 35 days in media lacking these supplements. Autoradiography demonstrated that 55% of the luminal sperm present in explants cultured 10 days had differentiated in vitro. Sperm from testes cultured 10-35 days were similar to sperm from freshly dissected testes with regard to motility and fecundity, and eggs fertilized with sperm from explant cultures developed normally into swimming tadpoles. The results demonstrate the feasibility of maintaining vertebrate spermatogenesis in culture and suggest that in vitro analysis of Xenopus spermatogenesis using defined media may provide important insights into the evolution of regulatory mechanisms in spermatogenesis.

  6. Dorset Days

    E-print Network

    Macfarlane, Alan

    2015-06-16

    away. From my teens I had the idea that I would try to construct an ‘archive’ of my life and perhaps that of my relatives. So I hoarded toys, photographs, letters, writings, anything I could. This desire to hold on to the past is shown in an essay... he came to meet me in Calcutta on my first trip to Assam and gave me all the change from every transaction. Or when we went on fishing expeditions and a fishing holiday together in my teens. And I remember wonderful days on the Croft...

  7. 3-Day versus 5-Day Course of Intravenous Antibiotics for Suspected Early Onset Neonatal Sepsis: A Randomized Controlled Trial

    PubMed Central

    Pasha, Yadollah Zahed; Ahmadpour-Kacho, Mussa; Behmadi, Reza; Jahangir, Tahereh

    2014-01-01

    Objective: The objective of this randomized controlled trial was to compare the treatment failure of suspected early onset neonatal sepsis with either 3-day or 5-day course of empirical antibiotic therapy. Methods: Infants with birth weight over 1500 g and/or gestational age over 34 weeks within 7 days postnatal age with clinical symptoms of neonatal sepsis received empirical antibiotics (Ampicillin + Amikacin) in two neonatal intensive care units. After 72 hours if the result of blood culture was negative and symptoms resolved they were randomly allocated to 3-day or 5-day groups. The main outcome was treatment failure which was defined as reappearance of symptoms of sepsis within two weeks after discontinuation of antibiotics. Infants with congenital anomalies, localized infections, asphyxia, those undergoing surgery or when serum C-reactive protein levels remained abnormal despite treatment, were not included. Randomization was accomplished with simple randomization procedure. Findings: Sixty patients were randomized in a 1:1 ratio to either group. Baseline characteristics were similar between two groups. The follow-up period was 2 weeks with no lost to follow-up. One infant in 3-day group had treatment failure compared with no treatment failure in 5-day group (P=0.5). No serious harm was observed due to our empirical antibiotic regimen. Conclusion: The results of this study indicated no evidence that treatment failure differs between 3-day and 5-day course antibiotic therapy for suspected early onset uncomplicated neonatal sepsis in late preterm and term newborns. PMID:26019770

  8. Pesticides Safely For more information on selection, planting, cultural

    E-print Network

    Liskiewicz, Maciej

    Applying Pesticides Safely For more information on selection, planting, cultural practices and instructions. Observe the days-to- harvest period for fruits and vegetables to the letter. Use pesticides only and use of pesticides are regulat- ed by the United States Environmental Protection Agency

  9. Hippocampal culture stimulus with 4-megahertz ultrasound

    NASA Astrophysics Data System (ADS)

    Muratore, Robert; LaManna, Justine K.; Lamprecht, Michael R.; Morrison, Barclay, III

    2012-10-01

    Among current modalities, ultrasound uniquely offers both millisecond and millimeter accuracy in noninvasively stimulating brain tissue. In addition, by sweeping the ultrasound beam within the refractory period of the neuronal tissue, ultrasonic neuromodulation can be adapted to target extended or multiply connected regions with quasi-simultaneity. Towards the development of this safe brain stimulus technique, the response of rat hippocampal cultures to ultrasound was investigated. Hippocampal slices, 0.4-mm thick, were obtained from 8-day old Sprague Dawley rats and cultured for 6 days. The in vitro cultures were exposed to multiple 100-ms 4.04-MHz ultrasound pulses from a 42-mm diameter, 90-mm spherical cap transducer. Peak pressure ranged from 0 through about 77 kPa. Responses in the form of electrical potentials from a sixty channel electrode array were digitized and recorded. The DG and CA1 regions of the hippocampus exhibited similar ultrasonically-evoked field potentials.

  10. Stennis Space Center celebrates Diversity Day

    NASA Technical Reports Server (NTRS)

    2009-01-01

    Kendall Mitchell of the Naval Oceanographic Office (right) learns about the culture of Bolivia from Narda Inchausty, president of the Foreign Born Wives Association in Slidell, La., during 2009 Diversity Day events at NASA's John Stennis Space Center. Stennis hosted Diversity Day activities for employees on Oct. 7. The day's events included cultural and agency exhibits, diversity-related performances, a trivia contest and a classic car and motorcycle show. It also featured the first-ever sitewide Stennis Employee Showcase.

  11. Visions of alterity: the impact of cross-cultural contacts on european self-understanding in the pre-enlightenment period

    E-print Network

    Bashir, Hassan

    2009-05-15

    cultural interaction, and the resultant global diversity. However, this response must not be an attempt to domesticate the Other and provide an even more universalistic, Eurocentric explanation of dilemmas, which CPT forcefully claims are human... critique of western rationalism.14 CPT makes such a reading of Qutb?s ideas possible by demanding that mechanistic Eurocentric models of interpretation be avoided when evaluating non- western ideas. In a sense, therefore, CPT identifies inherent...

  12. Cultural Diplomacy in Europe.

    ERIC Educational Resources Information Center

    Haigh, Anthony

    The evolution of European government activities in the sphere of international cultural relations is examined. Section 1 describes the period between World War I and World War II when European governments tried to enhance their prestige and policies by means of cultural propaganda. Section 2 analyzes the period during World War II when the…

  13. Let's Celebrate! Canada's Special Days.

    ERIC Educational Resources Information Center

    Parry, Caroline

    Designed for children ages 8 to 13, this teaching resource presents an explanation of seasons, calendars, and why people celebrate particular days. The four seasons are discussed. Canada's national holidays, and the seasonal, social and religious holidays celebrated by diverse Canadian culture groups are described. A separate section presents…

  14. Meat Consumption Culture in Ethiopia

    PubMed Central

    Jo, Cheorun

    2014-01-01

    The consumption of animal flesh food in Ethiopia has associated with cultural practices. Meat plays pivotal and vital parts in special occasions and its cultural symbolic weight is markedly greater than that accorded to most other food. Processing and cooking of poultry is a gender based duty and has socio-cultural roles. Ethiopians are dependent on limited types of animals for meats due to the taboo associated culturally. Moreover, the consumption of meat and meat products has a very tidy association with religious beliefs, and are influenced by religions. The main religions of Ethiopia have their own peculiar doctrines of setting the feeding habits and customs of their followers. They influence meat products consumption through dictating the source animals that should be used or not be used for food, and scheduling the days of the years in periodical permeation and restriction of consumptions which in turn influences the pattern of meat consumption in the country. In Ethiopia, a cow or an ox is commonly butchered for the sole purpose of selling within the community. In special occasions, people have a cultural ceremony of slaughtering cow or ox and sharing among the group, called Kircha, which is a very common option of the people in rural area where access of meat is challenging frequently.

  15. Embryonic mouse pre-metatarsal development in organ culture

    NASA Technical Reports Server (NTRS)

    Klement, B. J.; Spooner, B. S.

    1993-01-01

    Embryonic mouse pre-metatarsals were removed from embryos at 13 days of gestation and cultured in a defined, serum-free medium for up to 15 days. By histological analysis, we observe that the cultured pre-metatarsal tissue undergoes a similar developmental profile as pre-metatarsals growing normally in vivo. The initial mesenchyme condensation regions undergo differentiation and morphogenesis to form distinct rods made up of cartilage tissue. A marker of this differentiation step is the synthesis of type II collagen. Metabolic labelling, pepsin digestion, SDS-PAGE, and autoradiography were used to demonstrate this protein when cartilage tissue is present in the cultures. After additional culture time, terminal chondrocyte differentiation and morphogenesis take place in specific regions of the cartilage rods to form bands of hypertrophied chondrocytes. One marker of this differentiation step is the synthesis of the enzyme alkaline phosphatase. We have measured the activity of this enzyme throughout the culture period and see a substantial increase at the time of terminal chondrocyte differentiation. Another feature of hypertrophied chondrocytes is that the matrix around the cells becomes calcified. Calcified matrix in our cultured pre-metatarsals was visualized by staining with alizarin red. By supplementing the defined culture medium with ITS, we observed that terminal chondrocyte differentiation took place in a shorter culture time. Supplementation of the medium with serum results in a similar acceleration of terminal differentiation, and, with additional culture time, an osteoid-like matrix forms around the central region of the rods.

  16. Non-spore forming eubacteria isolated at an altitude of 20,000 m in Earth's atmosphere: extended incubation periods needed for culture-based assays

    USGS Publications Warehouse

    Griffin, Dale W.

    2008-01-01

    On 13 August 2004, an atmospheric sample was collected at an altitude of 20,000 m along a west to east transect over the continental United States by NASA’s Stratospheric and Cosmic Dust Program. This sample was then shipped to the US Geological Survey’s Global Desert Dust program for microbiological analyses. This sample, which was plated on a low nutrient agar to determine if cultivable microorganisms were present, produced 590 small yellow to off-white colonies after approximately 7 weeks of incubation at room-temperature. Of 50 colonies selected for identification using 16S rRNA sequencing, 41 belonged to the family Micrococcaceae, seven to the family Microbacteriaceae, one to the genus Staphylococcus, and one to the genus Brevibacterium. All of the isolates identified were non-spore-forming pigmented bacteria, and their presence in this sample illustrate that it is not unusual to recover viable microbes at extreme altitudes. Additionally, the extended period required to initiate growth demonstrates the need for lengthy incubation periods when analyzing high-altitude samples for cultivable microorganisms.

  17. The New Jersey Security and Financial Empowerment Act ("NJ SAFE Act"), P.L. 2013, c.82, provides that certain employees are eligible to receive an unpaid leave of absence, for a period not to exceed 20 days in

    E-print Network

    by both the NJ SAFE Act and the Family Leave Act, N.J.S.A. 34:11B-1 et seq., or the federal Family of no less than one day. The unpaid leave shall run concurrently with any paid vacation leave, personal leave

  18. Glycosphingolipid biosynthesis in adult mouse kidney cultures

    SciTech Connect

    Lyerla, T.A.; Gross, S.K.; McCluer, R.H.

    1986-05-01

    Primary kidney cultures from C57BL/6J mice, six weeks of age or older, were produced using D-valine medium to select for epithelial cell growth. After allowing cells to attach and proliferate for one week following plating, medium was changed once per week. Cells formed nearly confluent monolayers during the second week of culture. The cultured cells contained all of the glycosphingolipids seen in the adult kidney, as analyzed by HPLC as the perbenzoyl derivatives. Glucosylceramide, however, was highly predominant in the cultured cells, whereas dihexosyl- and trihexosylceramides predominate in the intact kidney. Sex differences in glycolipid contents found in the intact kidney were also apparent in these cultured cells: greater amounts of nonhydroxy fatty acid (Nfa) containing galactosylceramide and Nfa digalactosylceramide were present in the male cells. Neutral glycosphingolipids were labeled after two weeks in culture using /sup 3/H-labeled palmitate. The /sup 3/H-palmitate was incorporated into all of the glycolipids within two hours of labeling, and after three days of chase with unlabeled palmitate, some redistribution into higher glycosphingolipids was apparent. Hence, adult mouse kidney cells in D-valine medium retain their differentiated characteristics for a sufficient period of time to allow investigation of glycolipid syntheses in monolayer cultures of epithelial cells derived from this organ.

  19. Bioactivity of water soluble extracts and some characteristics of white cheese during the ripening period as effected by packaging type and probiotic adjunct cultures.

    PubMed

    Erkaya, Tuba; ?engul, Mustafa

    2015-02-01

    In this study, the chemical composition, proteolysis and in vitro angiotensin-converting enzyme-(ACE)-inhibitory and antioxidant activities of white cheeses made using probiotic adjunct cultures (Bifidobacterium bifidum DSMZ 20456 and Lactobacillus acidophilus DSMZ 20079) were investigated. The cheeses were ripened in a vacuum package or brine for 120 d at 4 °C. The cheese samples maintained the probiotic characteristics of the viable cells as >106 cfu/g even after ripening for 120 d. The proteolysis degrees in terms of water-soluble nitrogen/total nitrogen (WSN/TN), trichloroacetic acid-soluble nitrogen/total nitrogen (TCA-SN/TN) and phosphotungstic acid-soluble nitrogen/total nitrogen (PTA-SN/TN) values in the cheeses increased throughout the ripening. The highest levels of proteolysis were found in cheese made using Lb. acidophilus DSMZ 20079 and ripened in a vacuum package. ACE-inhibitory activity of the water soluble extracts (WSEs) of the cheeses increased significantly (P < 0·05) throughout the ripening (IC50 values 82·78-140·99 ?g/ml). Use of Lb. acidophilus DSMZ 20079 and packaging under vacuum significantly increased the percentage of ACE inhibiting activity. WSEs had DPPH scavenging activity (the IC50 values were 2·41-5·39 mg/ml and the inhibition values were 5·10-10·38%), increasing up to 60 d ripening. In the present study, it was observed that Lb. acidophilus DSMZ 20079 was more effective than Bifido. bifidum DSMZ 20456 in terms of the cheese characteristics investigated. PMID:25592630

  20. Insulin stimulates choline acetyltransferase activity in cultured embryonic chicken retina neurons

    SciTech Connect

    Kyriakis, J.M.; Hausman, R.E.; Peterson, S.W.

    1987-11-01

    The effect of insulin on the appearance of the enzyme choline acetyltransferase in embryonic chicken retina neurons cultured in defined medium was studied. In the presence of a minimal level of insulin (1 ng/ml), ChoAcT activity increased with time in culture. A correspondence between the insulin concentration in the defined medium (1-100 ng/ml) and both the rate of increase and maximum attained level of ChoAcT activity was observed. Maximal ChoAcT activity was 2- to 3-fold greater in cells cultured in the presence of 100 ng of insulin per ml than in cells cultured in the presence of 1 ng of insulin per ml. To elicit maximum ChoAcT activity, insulin at 100 ng/ml was required in the medium for only the first 4 days of the culture period, at which time insulin could be reduced to maintenance levels (10 ng/ml) without affecting ChoAcT activity. Insulin binding assays performed during a 7-day culture period revealed that irrespective of the /sup 125/I-insulin concentration in the medium during culture, cell-surface insulin receptors decreased by approx. = 90% between 4 and 7 days in culture. This decrease in insulin binding corresponded to the observed decrease in the sensitivity of ChoAcT activity to insulin. The findings suggest that insulin plays a role in mediating cholinergic differentiation in the embryonic chicken retina.

  1. Irregular Periods

    MedlinePLUS

    ... Safely Irregular Periods KidsHealth > Teens > Sexual Health > For Girls > Irregular Periods Print A A A Text Size ... after the last one. The Menstrual Cycle Most girls get their first period between the ages of ...

  2. 46 CFR Sec. 5 - Idle status period.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...from Military Sea Transportation Service notwithstanding the fifteen (15) days minimum period as provided for in paragraph (b...anticipated that the minimum period of inactivity will exceed fifteen (15) days, due, but not limited to: (1)...

  3. Day 1 Day 2 Day 3 Day 4 Day 5 Cardiology Dermatology Endocrine Epidemiology and Ethics GI

    E-print Network

    Finley Jr., Russell L.

    Day 1 Day 2 Day 3 Day 4 Day 5 Cardiology Dermatology Endocrine Epidemiology and Ethics GI 6:00 a Day 11 Day 12 Day 13 Day 14 Day 15 Pediatrics Psychiatry Pulmonary Renal/GU Emergency Med 6:00 a.m. -7 and throughly study text 10:00-10:30 Break Break Break Break Break 10:30-12:30 p.m. Q-Bank Pediatrics Q

  4. 7-29 A coal-burning power plant produces 300 MW of power. The amount of coal consumed during a one-day period and the rate of air flowing through the furnace are to be determined.

    E-print Network

    Bahrami, Majid

    7-11 7-29 A coal-burning power plant produces 300 MW of power. The amount of coal consumed during The heating value of the coal is given to be 28,000 kJ/kg. Analysis (a) The rate and the amount of heat inputs'tQQ The amount and rate of coal consumed during this period are kg/s48.33 s360024 kg10893.2 MJ/kg28 MJ101.8 6

  5. Blood culture cross contamination associated with a radiometric analyzer

    SciTech Connect

    Griffin, M.R.; Miller, A.D.; Davis, A.C.

    1982-04-01

    During a 9-day period in August 1980 in a New Jersey hospital, three pairs of consecutively numbered blood cultures from different patients were identified as positive for the same organism, for each pair, both cultures were positive in the same atmosphere, both organisms had the same sensitivities, and the second of each pair grew at least 2 days after the first and was the only positive blood culture obtained from the patient. When the hospital laboratory discontinued use of its radiometric culture analyzer for 15 days, no more consecutive pairs of positive cultures occurred. Subsequent use of the machine for 9 days with a new power unit but the original circuit boards resulted in one more similar consecutive pair (Staphylococcus epidermidis). After replacement of the entire power unit, there were no further such pairs. Examination of the machine by the manufacturer revealed a defective circuit board which resulted in inadequate needle sterilization. Laboratories which utilize radiometric analyzers should be aware of the potential for cross contamination. Recognition of such events requires alert microbiologists and infection control practitioners and a record system in the bacteriology laboratory designed to identify such clusters.

  6. Immunoregulatory activity in supernatants from cultures of normal human trophoblast cells of the first trimester.

    PubMed

    Sanyal, M K; Brami, C J; Bischof, P; Simmons, E; Barnea, E R; Dwyer, J M; Naftolin, F

    1989-08-01

    Supernatants from isolated trophoblast cell cultures (trophoblastic fluid) derived from first-trimester human placentas were assessed for immunoregulatory activity. Trophoblastic fluid at different days of culture consistently inhibited the blast transformation of allogenic lymphocytes. This suppressor effect had no apparent correlation with biosynthesis of human chorionic gonadotropin by trophoblast cells, since this hormone was secreted into the culture fluid only for the initial 3 days. However, the culture fluids of such purified trophoblast cells contained an immunosuppressive factor, pregnancy-associated plasma protein A, which was measurable throughout the culture period of 8 days. The presence of pregnancy-associated plasma protein A in significant amounts in trophoblastic fluid collected at daily intervals indicated a continuous secretion ability of pregnancy-associated plasma protein A by trophoblast cells in culture parallel to the suppressive immunoregulatory effect of the fluid. Such immunosuppressive effect was absent in the culture fluids of control BeWo malignant trophoblast cells; the BeWo cell culture fluids had markedly reduced levels of pregnancy-associated plasma protein A. The culture supernatant of normal trophoblast cells of placentas from first-trimester pregnancy activated in vitro the generation of a population of suppressor lymphocytes. This effect is generally considered responsible for immunologic tolerance. Therefore demonstration of immunosuppressive effects and the presence of relatively high levels of pregnancy-associated plasma protein A in trophoblastic fluid indicate that such proteins secreted by the trophoblast cells may be important in the local immunoregulatory processes of the fetal allograft. PMID:2475020

  7. 42 CFR 418.21 - Duration of hospice care coverage-Election periods.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...elect to receive hospice care during one or more of... (1) An initial 90-day period; (2) A subsequent 90-day period; or (3) An...number of subsequent 60-day periods. (b) The periods of care are available in...

  8. 42 CFR 418.21 - Duration of hospice care coverage-Election periods.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...elect to receive hospice care during one or more of... (1) An initial 90-day period; (2) A subsequent 90-day period; or (3) An...number of subsequent 60-day periods. (b) The periods of care are available in...

  9. 42 CFR 418.21 - Duration of hospice care coverage-Election periods.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...elect to receive hospice care during one or more of... (1) An initial 90-day period; (2) A subsequent 90-day period; or (3) An...number of subsequent 60-day periods. (b) The periods of care are available in...

  10. 42 CFR 418.21 - Duration of hospice care coverage-Election periods.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...elect to receive hospice care during one or more of... (1) An initial 90-day period; (2) A subsequent 90-day period; or (3) An...number of subsequent 60-day periods. (b) The periods of care are available in...

  11. 42 CFR 418.21 - Duration of hospice care coverage-Election periods.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...elect to receive hospice care during one or more of... (1) An initial 90-day period; (2) A subsequent 90-day period; or (3) An...number of subsequent 60-day periods. (b) The periods of care are available in...

  12. TISSUE CULTURE AS A METHOD FOR EVALUATING THE BIOTRANSFORMATION OF XENOBIOTICS BY PLANTS

    EPA Science Inventory

    Suspension cultures of Rosa cv. Paul's scarlet were used as a model system to examine the metabolism of l,3-dinitrobenzene (DNB), an industrial waste compound. n a three-day period, 90% of the DNB supplied (96 nmoles) was metabolized by approximately 12 grams (fresh weight) of ce...

  13. Stress-Induced Changes in the Expression of the Clock Protein PERIOD1 in the Rat Limbic Forebrain and Hypothalamus: Role of Stress Type, Time of Day, and Predictability

    PubMed Central

    Al-Safadi, Sherin; Al-Safadi, Aya; Branchaud, Marie; Rutherford, Spencer; Dayanandan, Arun; Robinson, Barry; Amir, Shimon

    2014-01-01

    Stressful events can disrupt circadian rhythms in mammals but mechanisms underlying this disruption remain largely unknown. One hypothesis is that stress alters circadian protein expression in the forebrain, leading to functional dysregulation of the brain circadian network and consequent disruption of circadian physiological and behavioral rhythms. Here we characterized the effects of several different stressors on the expression of the core clock protein, PER1 and the activity marker, FOS in select forebrain and hypothalamic nuclei in rats. We found that acute exposure to processive stressors, restraint and forced swim, elevated PER1 and FOS expression in the paraventricular and dorsomedial hypothalamic nuclei and piriform cortex but suppressed PER1 and FOS levels exclusively in the central nucleus of the amygdala (CEAl) and oval nucleus of the bed nucleus of the stria terminalis (BNSTov). Conversely, systemic stressors, interleukin-1? and 2-Deoxy-D-glucose, increased PER1 and FOS levels in all regions studied, including the CEAl and BNSTov. PER1 levels in the suprachiasmatic nucleus (SCN), the master pacemaker, were unaffected by any of the stress manipulations. The effect of stress on PER1 and FOS was modulated by time of day and, in the case of daily restraint, by predictability. These results demonstrate that the expression of PER1 in the forebrain is modulated by stress, consistent with the hypothesis that PER1 serves as a link between stress and the brain circadian network. Furthermore, the results show that the mechanisms that control PER1 and FOS expression in CEAl and BNSTov are uniquely sensitive to differences in the type of stressor. Finally, the finding that the effect of stress on PER1 parallels its effect on FOS supports the idea that Per1 functions as an immediate-early gene. Our observations point to a novel role for PER1 as a key player in the interface between stress and circadian rhythms. PMID:25338089

  14. Starter culture activity in refrigerated fermented soymilk.

    PubMed

    Garro, M S; de Valdez, G F; Oliver, G; de Giori, G S

    1999-07-01

    The refrigerated shelf life of soymilk fermented with single cultures of Lactobacillus fermentum, L. casei, Streptococcus salivarius subsp. thermophilus, and Bifidobacterium longum was evaluated. During storage at 4 degrees C for 28 days, the stability of the microflora differed markedly among the starter cultures. After 28 days, the average numbers of S. salivarius subsp. thermophilus decreased by two log cycles to 6.0 x 10(7) CFU/ml, whereas those of L. casei increased gradually by more than two log cycles to 4.6 x 10(9) CFU/ml. Numbers of B. longum and L. fermentum remained moderately high (8.7 x 10(8) CFU/ml and 3.7 x 10(8) CFU/ml, respectively) even after 28 days of storage. S. salivarius subsp. thermophilus and L. casei continued to metabolize sucrose during the storage period, but the pattern of consumption was different among the strains. The other starter cultures did not seem to have significant activity (P > 0.05) on the residual sugars. In most cases, L(+)-lactate predominated. PMID:10419278

  15. Carbon disulphide production in laboratory cultures of marine phytoplankton

    NASA Astrophysics Data System (ADS)

    Xie, Huixiang; Scarratt, Michael G.; Moore, Robert M.

    Carbon disulphide (CS 2) data were collected from axenic monocultures of six species of marine phytoplankton. The tested species included Chaetoceros calcitrans, Phaeodactylum tricornutum, Phaeocystis sp., Porphyridium purpureum, Synechococcus sp. and Isochrysis sp. For a period of between two weeks and forty days, substantial accumulation of CS 2 was found in the cultures of C. calcitrans, P. tricornutum and Phaeocystis sp., whereas the change of CS 2 concentration in the remaining cultures was insignificant. C. calcitrans had a potential for CS 2 production about 10 times higher than P. tricornutum or Phaeocystis sp. The formation of the compound was strongly dependent on the physiological state of the cultured species. More investigation is needed to elucidate the mechanisms responsible for the formation of this sulphur compound in these cultures.

  16. New Swedish Cultural Environment Protection.

    ERIC Educational Resources Information Center

    Ministry of Education and Cultural Affairs, Stockholm (Sweden).

    Current Swedish cultural policy was laid down in 1974. It was decided that one of the aims of that policy must be to ensure that earlier periods of history would be preserved and brought to life. The Government Bill (Prop. 1987/88:104) on protection of the cultural environment is concerned with helping the general public understand that cultural

  17. 24 CFR 58.21 - Time periods.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Time periods. 58.21 Section 58.21...: Environmental Review Procedures § 58.21 Time periods. All time periods in this part shall be counted in calendar days. The first day of a time period begins at 12:01 a.m. local time on the day following...

  18. 24 CFR 58.21 - Time periods.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Time periods. 58.21 Section 58.21...: Environmental Review Procedures § 58.21 Time periods. All time periods in this part shall be counted in calendar days. The first day of a time period begins at 12:01 a.m. local time on the day following...

  19. 24 CFR 58.21 - Time periods.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Time periods. 58.21 Section 58.21...: Environmental Review Procedures § 58.21 Time periods. All time periods in this part shall be counted in calendar days. The first day of a time period begins at 12:01 a.m. local time on the day following...

  20. 24 CFR 58.21 - Time periods.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Time periods. 58.21 Section 58.21...: Environmental Review Procedures § 58.21 Time periods. All time periods in this part shall be counted in calendar days. The first day of a time period begins at 12:01 a.m. local time on the day following...

  1. 24 CFR 58.21 - Time periods.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Time periods. 58.21 Section 58.21...: Environmental Review Procedures § 58.21 Time periods. All time periods in this part shall be counted in calendar days. The first day of a time period begins at 12:01 a.m. local time on the day following...

  2. The Mass Culture of Porphyridium cruentum

    PubMed Central

    Golueke, Clarence G.; Oswald, William J.

    1962-01-01

    A study was made of the effect of temperature, detention period, light intensity, and salinity on the growth rate and over-all light energy conversion efficiency of Porphyridium cruentum cultured on a medium consisting of concentrated sea water and sewage enriched with urea, chelated iron, and other additives. It was found that the optimal temperature was within the range of 21 to 26 C. Growth was retarded at temperatures less than 13 C, and completely inhibited above 31 C. Over-all light energy conversion efficiency increased from 2.24% at the 4-day detention period to 2.76% at the 10-day period. Conversion efficiency ranged from 5.8% at a light energy absorption rate of 8.2 cal:liter:min to 2.3% at 35 to 39 cal:liter:min. At salt concentrations less than 3.5%, Porphyridium could not successfully compete with other algae in open cultures. Salt concentrations as high as 4.6% had no inhibitory effect on its growth. In studies on nutrition, it was found that growth on a medium of salts used in formulating synthetic sea water dissolved in sewage was equal to that on a control medium consisting of concentrated sea water and sewage (see above). They showed that sewage contains a substance or substances essential for optimal growth. Vitamin B12 alone could not substitute for it. Images FIG. 1 PMID:13900025

  3. Adult Day Services

    MedlinePLUS

    A Smart Choice Adult Day Services Comparison At-a-Glance 1 Adult Day Services Assisted Living Home Care Nursing Homes Live at home with family ... supervision Nursing care available as needed during the day Flexibility to receive care only on days when ...

  4. Recent Innovations in Cultural Practices in the Mid-Atlantic Coast Region of the U. S.: Novel Systems for Increasing Fall Fruiting in Short-day Type Strawberry Cultivars and Opportunities for Out-of-Season Fruit Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Producing strawberry transplants from runner tips that were plugged about one month earlier (early July) than the standard time (early August) promoted fall flowering in some short-day strawberry cultivars. In 2002, 100 percent of ‘Chandler’ transplants produced in early July flowered in the fall,...

  5. Relationship of safety culture and process safety.

    PubMed

    Olive, Claire; O'Connor, T Michael; Mannan, M Sam

    2006-03-17

    Throughout history, humans have gathered in groups for social, religious, and industrial purposes. As the conglomeration of people interact, a set of underlying values, beliefs, and principles begins to develop that serve to guide behavior within the group. These "guidelines" are commonly referred to as the group culture. Modern-day organizations, including corporations, have developed their own unique cultures derived from the diversity of the organizational interests and the background of the employees. Safety culture, a sub-set of organizational culture, has been a major focus in recent years. This is especially true in the chemical industry due to the series of preventable, safety-related disasters that occurred in the late seventies and eighties. Some of the most notable disasters, during this time period, occurred at Bhopal, Flixborough, and Seveso. However, current events, like the September 11th terrorist attacks and the disintegration of the Columbia shuttle, have caused an assessment of safety culture in a variety of other organizations. PMID:16314040

  6. Opacification of lenses cultured in the presence of Pb

    PubMed Central

    Lin, C.; Isom, R.; Vaishnav, K.; Zigler, J.S.

    2010-01-01

    Purpose Occupational and environmental Pb-exposure is associated with protein aggregation diseases which typically present in elderly populations (Parkinsons and cataract). Post-translational processing of crystallins, the major structural proteins of the lens, is altered with short-term Pb-exposure in Fisher 344 rats. In addition, lenses from aged rats become opaque upon long-term exposure to Pb in organ culture. To explore the route to lens opacification in the presence of Pb, cultured lenses from young rats which exhibit higher metabolic activity in lens culture and are more susceptible to experimental cataract in vivo and in vitro were exposed to Pb and evaluated for morphological and biochemical alterations. Methods Following culture in Pb (as lead nitrate) for four days (in the presence/absence of oxidative challenge), lenses were examined for clarity, integrity of epithelial layer, and molecular stability including crystallin post-translational modification and choline transport. Clarity of lenses cultured with/without Pb for up to 8 days was assessed to determine if Pb exposure would accelerate opacification. Results Lenses cultured in Pb for four days exhibited epithelial abnormalities including epithelial cell multilayering and nuclei abnormalities with extension of the nucleated epithelial cells past the bow region. Alterations in crystallin post-translational modifications and decreased membrane transport of choline were noted without corresponding lens opacification or altered ?-crystallin chaperone activity. Lenses treated with Pb according to the same exposure protocol with subsequent challenge by hydrogen peroxide became opaque while the contralateral control lenses did not. Lenses which were cultured in the presence of Pb for longer periods with no subsequent oxidative insult exhibited lens failure at earlier time points than did the controls. Conclusions These data indicate that Pb-exposure can accelerate the degradation of the cultured lens through induction of epithelial cell abnormalities, induce structural protein modifications before opacity, and predispose the lens to opacification with subsequent oxidant challenge. PMID:21139692

  7. Post-translational control of collagen fibrillogenesis in mineralizing cultures of chick osteoblasts

    NASA Technical Reports Server (NTRS)

    Gerstenfeld, L. C.; Riva, A.; Hodgens, K.; Eyre, D. R.; Landis, W. J.

    1993-01-01

    Cultured osteoblasts from chick embryo calvaria were used as a model system to investigate the post-translational extracellular mechanisms controlling the macroassembly of collagen fibrils. The results of these studies demonstrated that cultured osteoblasts secreted a collagenous extracellular matrix that assembled and mineralized in a defined temporal and spatial sequence. The assembly of collagen occurred in a polarized fashion, such that successive orthogonal arrays of fibrils formed between successive cell layers proceeding from the culture surface toward the media. Mineralization followed in the same manner, being observed first in the deepest and oldest fibril layers. Collagen fibrillogenesis, the kinetics of cross-link formation, and collagen stability in the extracellular matrix of the cultures were examined over a 30 day culture period. Between days 8 and 12 in culture, collagen fibril diameters increased from < 30 nm to an average of 30-45 nm. Thereafter, diameters ranged in size from 20 to 200 nm. Quantitation of the collagen cross-linking residues, hydroxylysyl pyridinoline (HP) and lysyl pyridinoline (LP), showed that these mature cross-links increased from undetectable levels to concentrations found in normal chick bone. Analysis of the kinetics of their formation by pulse-chase labeling the cultures with [3H]lysine showed a doubling time of approximately 5 days. The relationships between cross-link formation, fibrillogenesis, and collagen stability were examined in cultures treated with beta-aminopropionitrile (beta-APN), a potent inhibitor of lysyl oxidase and cross-link formation. In beta-APN-treated cultures, total collagen synthesis was increased twofold, with no change in mRNA levels for type I collagen, whereas the amount of collagen accumulated in the cell layer was decreased by 50% and mineral deposition was reduced. The rate of collagen retention in the matrix was assessed by pulse-chase analysis of [3H]proline over a 16 day period in control and beta-APN-treated cultures. In control cultures, about 20% of the labeled collagen was lost from the cell layers over a 16 day period compared with > 80% in the presence of beta-APN. The beta-APN-treated cultures also showed a wider diversity of fibril diameters with a median in the > 45-60 nm range. In summary, these data suggest that cross-linking and assembly of collagen fibrils secreted by osteoblasts in vitro occur in a fashion similar to that found in vivo. The rate of cross-link formation is relatively constant and may be correlated with increasing collagen mass.(ABSTRACT TRUNCATED AT 400 WORDS).

  8. Mouse Pancreas Tissue Slice Culture Facilitates Long-Term Studies of Exocrine and Endocrine Cell Physiology in situ

    PubMed Central

    Marciniak, Anja; Selck, Claudia; Friedrich, Betty; Speier, Stephan

    2013-01-01

    Studies on pancreatic cell physiology rely on the investigation of exocrine and endocrine cells in vitro. Particularly, in the case of the exocrine tissue these studies have suffered from a reduced functional viability of acinar cells in culture. As a result not only investigations on dispersed acinar cells and isolated acini were limited in their potential, but also prolonged studies on pancreatic exocrine and endocrine cells in an intact pancreatic tissue environment were unfeasible. To overcome these limitations, we aimed to establish a pancreas tissue slice culture platform to allow long-term studies on exocrine and endocrine cells in the intact pancreatic environment. Mouse pancreas tissue slice morphology was assessed to determine optimal long-term culture settings for intact pancreatic tissue. Utilizing optimized culture conditions, cell specificity and function of exocrine acinar cells and endocrine beta cells were characterized over a culture period of 7 days. We found pancreas tissue slices cultured under optimized conditions to have intact tissue specific morphology for the entire culture period. Amylase positive intact acini were present at all time points of culture and acinar cells displayed a typical strong cell polarity. Amylase release from pancreas tissue slices decreased during culture, but maintained the characteristic bell-shaped dose-response curve to increasing caerulein concentrations and a ca. 4-fold maximal over basal release. Additionally, endocrine beta cell viability and function was well preserved until the end of the observation period. Our results show that the tissue slice culture platform provides unprecedented maintenance of pancreatic tissue specific morphology and function over a culture period for at least 4 days and in part even up to 1 week. This analytical advancement now allows mid -to long-term studies on the cell biology of pancreatic disorder pathogenesis and therapy in an intact surrounding in situ. PMID:24223842

  9. STS-90 Day 09 Highlights

    NASA Technical Reports Server (NTRS)

    1998-01-01

    On this ninth day of the STS-90 mission, the sleep period of the flight crew, Cmdr. Richard A. Searfoss, Pilot Scott D. Altman, and Mission Specialists Richard M. Linnehan, Dafydd Rhys Williams and Kathryn P. Hire, and Payload Specialists Jay C. Buckey and James A. Pawelczyk, is interrupted due to problems with equipment that removes carbon dioxide from the cabin atmosphere. Because of this, Columbia's crew went to bed about two hours later than scheduled.

  10. Day-1 chick development.

    PubMed

    Sheng, Guojun

    2014-03-01

    The first day of chick development takes place inside the mother hen (in utero), during which the embryo progresses from fertilization to late blastula/early gastrula formation. The salient features of developmental anatomy in this period are conserved among the sauropsids (birds and reptiles). Many of these features are also shared in prototherian (monotreme) embryos, whereas metatherian (marsupial) and eutherian (placental) embryos display significant variations. Important for understanding the evolution of early development in amniotes, the knowledge of cellular and molecular mechanisms regulating in utero chick development may also offer valuable insight into early lineage specification in prototherians and conserved features in mammalian early development. This commentary provides a snapshot of what is currently known about intrauterine chick development and identifies key issues that await further clarification, including the process of cellularization, allocation of maternal determinants, zygotic gene activation, mid-blastula transition, cell layer increase and reduction, radial symmetry breaking, early lineage segregation, and role of yolk syncytium in early patterning. PMID:24550174

  11. The effect of sodium salicylate on the rat embryo in culture: an in vitro model for the morphological assessment of teratogenicity.

    PubMed Central

    McGarrity, C; Samani, N; Beck, F; Gulamhusein, A

    1981-01-01

    The optimum teratogenic dose of subcutaneously administered sodium salicylate was determined in vivo at 9.5 days of gestation. Fetuses from rats injected with this dose at 9.5 days were examined at 11.5 days and its propensities for producing resorption as well as deformities noted. Next, maternal serum levels 3-3.5 hours and 18.5 hours after salicylate injection were determined. Having established the feasibility of determining an effect due to salicylate at 11.5 days, after injection at 9.5 days, the following experiments were performed in vitro in animals cultured between 9.5 and 11.5 days by the method of New et al. (1976a): (1) Sodium salicylate was added to the culture serum, at levels equivalent to those obtained 3-3.5 hours after maternal injection of the optimum teratogenic dose, for 24 of the 48 hours culture period. (2) Rats were cultured for the first 24 hours of the 48 hours culture period in serum taken from rats injected 3.5 hours previously with the optimum teratogenic dose. (3) Rats were cultured for 24 out of 48 hours in serum from animals which had been injected with the optimum teratogenic dose 18.5 hours before bleeding. (4) Rats were cultured for 24 out of 48 hours in serum containing salicylate added to make up levels normally associated with (3). (5) A control culture was performed. The experiment indicates great similarity between the results obtained from animals cultured in serum with salicylate added and results from culture in serum of salicylate treated rats. It appears therefore that sodium salicylate - acting directly on the feto-placental unit - is the active teratogen rather than any of its metabolites. Images Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 PMID:7333953

  12. Safeguards Culture

    SciTech Connect

    Frazar, Sarah L.; Mladineo, Stephen V.

    2012-07-01

    The concepts of nuclear safety and security culture are well established; however, a common understanding of safeguards culture is not internationally recognized. Supported by the National Nuclear Security Administration, the authors prepared this report, an analysis of the concept of safeguards culture, and gauged its value to the safeguards community. The authors explored distinctions between safeguards culture, safeguards compliance, and safeguards performance, and evaluated synergies and differences between safeguards culture and safety/security culture. The report concludes with suggested next steps.

  13. Riley-Day syndrome

    MedlinePLUS

    Riley-Day syndrome is an inherited disorder that affects nerves throughout the body. ... Riley-Day syndrome is passed down through families (inherited). A person must inherit a copy of the defective gene ...

  14. First Day of Life

    MedlinePLUS

    ... With Bullies Pregnant? What to Expect The First Day of Life KidsHealth > Parents > Pregnancy & Newborn Center > Childbirth > ... Continue What Your Baby Does on the First Day Many parents are surprised to see how alert ...

  15. Every Day Is Mathematical

    ERIC Educational Resources Information Center

    Barger, Rita H.; Jarrah, Adeeb M.

    2012-01-01

    March 14 is special because it is Pi Day. Mathematics is celebrated on that day because the date, 3-14, replicates the first three digits of pi. Pi-related songs, websites, trivia facts, and more are at the fingertips of interested teachers and students. Less celebrated, but still fairly well known, is National Metric Day, which falls on October…

  16. Growing degree day calculator

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Degree-day benchmarks indicate discrete biological events in the development of insect pests. For the Sparganothis fruitworm, we have isolated all key development events and linked them to degree-day accumulations. These degree-day accumulations can greatly improve treatment timings for cranberry IP...

  17. Defrosting Polar Dunes--Changes Over a 26-Day Period

    NASA Technical Reports Server (NTRS)

    1999-01-01

    As the retreat of the south polar winter frost cap became visible in June 1999, high resolution images from the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) began to show dark spots forming on the surfaces of frost-covered sand dunes. Immediately, the MOC science team began to plan to observe several dune fields more than once, should that opportunity arise, so that the evolution of these dark spots could be documented and studied. Such work will eventually lead to abetter understanding of how the martian polar caps retreat as winter ends and spring unfolds in each hemisphere.

    MGS is in a polar orbit, which means that, unlike many other places on Mars, the spacecraft has more opportunities to take pictures of the same place. Dune fields near 87o latitude can be repeatedly viewed; dunes near the equator are not likely to be photographed more than once during the entire MGS mission.

    The pictures presented here show changes on a set of nearly pear-shaped sand dunes located on the floor of an unnamed crater at 59oS, 353oW. The picture on the left shows the dunes as they appeared on June 19, 1999, the picture on the right shows the same dunes on July 15, 1999. The dark spots in the June 19picture--indicating areas where frost has sublimed away--became larger by July 15th. In addition, new spots had appeared as of mid-July. If possible, these dunes will be photographed by MOC again in mid-August and each month until the frost is gone.

    The pictures shown in (B) (above) are expanded views of portions of the pictures in (A). The 200 meter scale bar equals 656 feet; the 100 meter bar is 328 feet (109 yards) long. All images are illuminated from the upper left; north is toward the upper right.

    Malin Space Science Systems and the California Institute of Technology built the MOC using spare hardware from the Mars Observer mission. MSSS operates the camera from its facilities in San Diego, CA. The Jet Propulsion Laboratory's Mars Surveyor Operations Project operates the Mars Global Surveyor spacecraft with its industrial partner, Lockheed Martin Astronautics, from facilities in Pasadena, CA and Denver, CO.

  18. Every Day Is National Lab Day

    ERIC Educational Resources Information Center

    Bull, Glen

    2010-01-01

    President Barack Obama recently issued a call for increased hands-on learning in U.S. schools in an address at the National Academy of Sciences. Obama concluded that the future of the United States depends on one's ability to encourage young people to "create, and build, and invent." In this article, the author discusses National Lab Day (NLD)…

  19. EASTERN & ORIENTAL EXPRESS DAY BY DAY ITINERARY

    E-print Network

    Toronto, University of

    the passing scenery. Day 9 ~ Eastern & Oriental Express/ Singapore Disembark the train in Singapore. Excursion: Singapore. Explore Merlion Park and see the iconic Merlion statue of a lion- headed fish. Drive past for purification ceremonies. Then journey to Ubud Monkey Forest, an important monkey habitat located in a small

  20. Long term organ culture of human prostate tissue in a NASA-designed rotating wall bioreactor

    NASA Technical Reports Server (NTRS)

    Margolis, L.; Hatfill, S.; Chuaqui, R.; Vocke, C.; Emmert-Buck, M.; Linehan, W. M.; Duray, P. H.

    1999-01-01

    PURPOSE: To maintain ex vivo integral prostatic tissue including intact stromal and ductal elements using the NASA-designed Rotating Wall Vessel (RWV) which maintains colocalized cells in an environment that promotes both three-dimensional cellular interactions together with the uniform mass transfer of nutrients and metabolic wastes. MATERIALS AND METHODS: Samples of normal prostate were obtained as a byproduct of transurethral prostatectomy or needle biopsy. Prostatic tissue dissected into small 1 x 1 mm. blocks was cultured in the Rotating Wall Vessel (RWV) Bioreactor for various time periods and analyzed using histological, immunochemical, and total cell RNA assays. RESULTS: We report the long term maintenance of benign explanted human prostate tissue grown in simple culture medium, under the simulated microgravity conditions afforded by the RWV bioreactor. Mesenchymal stromal elements including blood vessels and architecturally preserved tubuloglandular acini were maintained for a minimum of 28 days. Cytokeratins, vimentin and TGF-beta2 receptor and ligand were preserved through the entire culture period as revealed by immunocytochemistry. Prostatic acid phosphatase (PAP) was continuously expressed during the culture period, although somewhat decreased. Prostatic specific antigen (PSA) and its transcript were down regulated over time of culture. Prostatic carcinoma cells from the TSU cell line were able to invade RWV-cultured benign prostate tissue explants. CONCLUSIONS: The RWV bioreactor represents an additional new technology for culturing prostate tissue for further investigations concerning the basic physiology and pathobiology of this clinically important tissue.

  1. Development of 3D hydrogel culture systems with on-demand cell separation.

    PubMed

    Hamilton, Sharon K; Bloodworth, Nathaniel C; Massad, Christopher S; Hammoudi, Taymour M; Suri, Shalu; Yang, Peter J; Lu, Hang; Temenoff, Johnna S

    2013-04-01

    Recently there has been an increased interest in the effects of paracrine signaling between groups of cells, particularly in the context of better understanding how stem cells contribute to tissue repair. Most current 3D co-culture methods lack the ability to effectively separate two cell populations after the culture period, which is important for simultaneously analyzing the reciprocal effects of each cell type on the other. Here, we detail the development of a 3D hydrogel co-culture system that allows us to culture different cell types for up to 7 days and subsequently separate and isolate the different cell populations using enzyme-sensitive glues. Separable 3D co-culture laminates were prepared by laminating PEG-based hydrogels with enzyme-degradable hydrogel adhesives. Encapsulated cell populations exhibited good segregation with well-defined interfaces. Furthermore, constructs can be separated on-demand upon addition of the appropriate enzyme, while cell viability remains high throughout the culture period, even after laminate separation. This platform offers great potential for a variety of basic cell signaling studies as the incorporation of an enzyme-sensitive adhesive interface allows the on-demand separation of individual cell populations for immediate analysis or further culture to examine persistence of co-culture effects and paracrine signaling on cell populations. PMID:23447378

  2. Enhanced bacoside production in shoot cultures of Bacopa monnieri under the influence of abiotic elicitors.

    PubMed

    Sharma, Munish; Ahuja, Ashok; Gupta, Rajinder; Mallubhotla, Sharada

    2015-01-01

    The effect of different abiotic elicitors [jasmonic acid, copper sulphate (CuSO4) and salicyclic acid] at varying concentrations on the stimulation of biomass and bacoside production in in vitro Bacopa monnieri shoot culture was studied. A systematic study conducted over a period of 35 days indicated that the maximum bacoside production (6.74 mg g(-1) dry weight (DW)) was obtained after a lag of 7 days and thereafter, the content decreased gradually to again increase at 28 days (5.91 mg g(-1) DW). Therefore, elicitation experiments were carried out over a period of 3, 6 and 9 days. The shoot cultures treated with 45 mg L(-1) of CuSO4 exhibited the highest bacoside content of 8.73 mg g(-1) DW (?1.42-fold higher) than in control cultures (6.14 mg g(-1) DW). This study indicates the effectiveness of abiotic elicitation on bacoside production in in vitro shoot cultures of this medicinally important herb known for its memory-enhancing properties. PMID:25485652

  3. Cultural psychology.

    PubMed

    Heine, Steven J; Ruby, Matthew B

    2010-03-01

    Humans are a cultural species, constantly navigating a complex web of culturally bound practices, norms, and worldviews. This article provides a brief overview of the relatively young field of cultural psychology, which investigates the many ways psychology and culture interweave with one another. Highlighting the cultural nature of the human species, it draws upon research on cultural evolution, enculturation, and developmental processes. This review further summarizes a number of cultural differences in how people perceive the self, and the behavioral consequences that follow from these differences, in the domains of internal and external attribution styles, motivations for self-enhancement, approach/avoidance, primary and secondary control, as well as motivations for distinctiveness and conformity. Additionally, the review discusses research on the intersection of culture and emotion, as well as cultural differences in cognition, perception, and reasoning. Copyright © 2010 John Wiley & Sons, Ltd. For further resources related to this article, please visit the WIREs website. PMID:26271239

  4. The inheritance of photoperiodic response in short day X day neutral cotton hybrids 

    E-print Network

    Waddle, Billy Mack

    1954-01-01

    Fourteen mares, 6 Arabians and 8 Thoroughbreds, were used to examine the efficacy of ultrasound for the evaluation of the mare's cervix during the estrous cycle. Within a 125-day period during the physiologic breeding season, mares were examined...

  5. Fifth day fits

    PubMed Central

    Goldberg, H James; Sheehy, Elizabeth M

    1982-01-01

    A 3-year prospective study to elucidate the cause of fifth day fits was carried out. A highly significant relationship with low cerebrospinal fluid zinc levels was found. Theoretical and practical considerations support the contention that fifth day fits are caused by an acute deficiency of zinc. PMID:7114883

  6. Rainy Day Activities.

    ERIC Educational Resources Information Center

    Texas Child Care, 1997

    1997-01-01

    Experienced caregivers plan ahead for rainy days. This article describes specific rainy day activities for young children, such as books and crafts to learn about rain (rain in a jar, making a rainbow), simple cooking activities (taffy pull, cinnamon candy tea), and games (mummy wrap, hunt the thimble, rain lotto). (EV)

  7. Science Challenge Day

    ERIC Educational Resources Information Center

    Siegel, Deborah

    2013-01-01

    Science fairs can be good motivators, but as extracurricular activities, they leave some students behind. However, by staging a Science Challenge Day at school, educators can involve all students in doing everything from choosing activities to judging projects. This article presents a model for running a successful Science Challenge Day. The…

  8. Day of the Dead

    ERIC Educational Resources Information Center

    Dann, Tammy; Murphy, Amy

    2012-01-01

    Foreign Language in Elementary School (FLES) teachers in the West Des Moines schools incorporate the Day of the Dead into the fourth grade curriculum each year. The teachers discuss the Day of the Dead celebration at the Art Center, and many ask for volunteers from fourth grade to participate in the event. Student presentations include a wide…

  9. Family Science Day

    ERIC Educational Resources Information Center

    McCubbins, Sara; Thomas, Bethany; Vetere, Michael

    2014-01-01

    This article describes a family-friendly science day event that encourages scientific discovery through hands-on activities, while also providing an opportunity to learn about scientific careers from actual research scientists and science educators, thereby raising awareness of the importance of STEM in our society. The one-day event bought…

  10. The Presidents' Day Game

    ERIC Educational Resources Information Center

    Maxwell, D. Jackson

    2008-01-01

    The history behind the holiday commonly called "Presidents' Day" is a bit confusing. It started as a federal holiday called Washington's Birthday. It was a day set aside to honor George Washington for his accomplishments as a founding father of the country. Later, many northern states began to recognize Abraham Lincoln's Birthday as well for his…

  11. [Infants in Day Care].

    ERIC Educational Resources Information Center

    Pawl, Jeree, Ed.; And Others

    1990-01-01

    This newsletter theme issue looks at infant day care models including those emphasizing early intervention with special needs infants. The lead article, "Infants in Day Care: Reflections on Experiences, Expectations and Relationships," by Jeree H. Pawl, stresses the importance of understanding infants' and toddlers' capacities and needs in…

  12. RED-LETTER DAYS

    EPA Science Inventory

    The word "red-letter" is an adjective meaning "of special significance." It's origin is from the practice of marking Christian holy days in red letters on calendars. The "red-letter days" to which I refer occurred while I was a graduate student of ...

  13. Cultural Neuroscience.

    PubMed

    Ames, Daniel L; Fiske, Susan T

    2010-06-01

    Cultural neuroscience issues from the apparently incompatible combination of neuroscience and cultural psychology. A brief literature sampling suggests, instead, several preliminary topics that demonstrate proof of possibilities: cultural differences in both lower-level processes (e.g. perception, number representation) and higher-order processes (e.g. inferring others' emotions, contemplating the self) are beginning to shed new light on both culture and cognition. Candidates for future cultural neuroscience research include cultural variations in the default (resting) network, which may be social; regulation and inhibition of feelings, thoughts, and actions; prejudice and dehumanization; and neural signatures of fundamental warmth and competence judgments. PMID:23874143

  14. Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices.

    PubMed

    Pagella, Pierfrancesco; Miran, Shayee; Mitsiadis, Tim

    2015-01-01

    Innervation plays a key role in the development, homeostasis and regeneration of organs and tissues. However, the mechanisms underlying these phenomena are not well understood yet. In particular, the role of innervation in tooth development and regeneration is neglected. Several in vivo studies have provided important information about the patterns of innervation of dental tissues during development and repair processes of various animal models. However, most of these approaches are not optimal to highlight the molecular basis of the interactions between nerve fibres and target organs and tissues. Co-cultures constitute a valuable method to investigate and manipulate the interactions between nerve fibres and teeth in a controlled and isolated environment. In the last decades, conventional co-cultures using the same culture medium have been performed for very short periods (e.g., two days) to investigate the attractive or repulsive effects of developing oral and dental tissues on sensory nerve fibres. However, extension of the culture period is required to investigate the effects of innervation on tooth morphogenesis and cytodifferentiation. Microfluidics systems allow co-cultures of neurons and different cell types in their appropriate culture media. We have recently demonstrated that trigeminal ganglia (TG) and teeth are able to survive for a long period of time when co-cultured in microfluidic devices, and that they maintain in these conditions the same innervation pattern that they show in vivo. On this basis, we describe how to isolate and co-culture developing trigeminal ganglia and tooth germs in a microfluidic co-culture system.This protocol describes a simple and flexible way to co-culture ganglia/nerves and target tissues and to study the roles of specific molecules on such interactions in a controlled and isolated environment. PMID:26327218

  15. Specimen Sample Preservation for Cell and Tissue Cultures

    NASA Technical Reports Server (NTRS)

    Meeker, Gabrielle; Ronzana, Karolyn; Schibner, Karen; Evans, Robert

    1996-01-01

    The era of the International Space Station with its longer duration missions will pose unique challenges to microgravity life sciences research. The Space Station Biological Research Project (SSBRP) is responsible for addressing these challenges and defining the science requirements necessary to conduct life science research on-board the International Space Station. Space Station will support a wide range of cell and tissue culture experiments for durations of 1 to 30 days. Space Shuttle flights to bring experimental samples back to Earth for analyses will only occur every 90 days. Therefore, samples may have to be retained for periods up to 60 days. This presents a new challenge in fresh specimen sample storage for cell biology. Fresh specimen samples are defined as samples that are preserved by means other than fixation and cryopreservation. The challenge of long-term storage of fresh specimen samples includes the need to suspend or inhibit proliferation and metabolism pending return to Earth-based laboratories. With this challenge being unique to space research, there have not been any ground based studies performed to address this issue. It was decided hy SSBRP that experiment support studies to address the following issues were needed: Fixative Solution Management; Media Storage Conditions; Fresh Specimen Sample Storage of Mammalian Cell/Tissue Cultures; Fresh Specimen Sample Storage of Plant Cell/Tissue Cultures; Fresh Specimen Sample Storage of Aquatic Cell/Tissue Cultures; and Fresh Specimen Sample Storage of Microbial Cell/Tissue Cultures. The objective of these studies was to derive a set of conditions and recommendations that can be used in a long duration microgravity environment such as Space Station that will permit extended storage of cell and tissue culture specimens in a state consistent with zero or minimal growth, while at the same time maintaining their stability and viability.

  16. Culturing Protozoa.

    ERIC Educational Resources Information Center

    Stevenson, Paul

    1980-01-01

    Compares various nutrient media, growth conditions, and stock solutions used in culturing protozoa. A hay infusion in Chalkey's solution maintained at a stable temperature is recommended for producing the most dense and diverse cultures. (WB)

  17. Throat Culture

    MedlinePLUS

    ... limited. Home Visit Global Sites Search Help? Throat Culture Share this page: Was this page helpful? Collecting | ... treatment | Getting results | see BLOOD SAMPLE Collecting A culture is a test that is often used to ...

  18. Gastric culture

    MedlinePLUS

    Gastric culture is a test to check a child's stomach contents for the bacteria that cause tuberculosis (TB). ... is placed in a special dish called a culture medium and watched for the growth of bacteria.

  19. A critical period for functional vestibular development in zebrafish

    NASA Technical Reports Server (NTRS)

    Moorman, Stephen J.; Cordova, Rodolfo; Davies, Sarah A.

    2002-01-01

    We have determined a critical period for vestibular development in zebrafish by using a bioreactor designed by NASA to simulate microgravity for cells in culture. A critical period is defined as the briefest period of time during development when stimulus deprivation results in long lasting or permanent sensory deficits. Zebrafish eggs were collected within 3 hours of being laid and fertilized. In experiment 1, eggs were placed in the bioreactor at 3, 24, 30, 36, 48, or 72 hours postfertilization (hPF) and maintained in the bioreactor until 96 hPF. In experiment 2, eggs were placed in the bioreactor immediately after they were collected and maintained in the bioreactor until 24, 36, 48, 60, 66, 72, or 96 hPF. Beginning at 96 hPF, all larvae had their vestibulo-ocular reflexes (VOR) evaluated once each day for 5 days. Only larvae that hatched from eggs that were placed in the bioreactor before 30 hPF in experiment 1 or removed from the bioreactor later than 66 hPF in experiment 2 had VOR deficits that persisted for at least 5 days. These data suggest a critical period for vestibular development in the zebrafish that begins before 30 hPF and ends after 66 hPF. To confirm this, zebrafish eggs were placed in the bioreactor at 24 hPF and removed at 72 hPF. VORs were evaluated in these larvae once each day for 5 days beginning at 96 hPF. These larvae had VOR deficits that persisted for at least 5 days. In addition, larvae that had been maintained in the bioreactor from 24 to 66 hPF or from 30 to 72 hPF, had only temporary VOR deficits. In a final experiment, zebrafish eggs were placed in the bioreactor at 3 hPF and removed at 96 hPF but the bioreactor was turned off from 24 hPF to 72 hPF. These larvae had normal VORs when they were removed from the bioreactor at 96 hPF. Taken as a whole, these data support the idea that there is a critical period for functional maturation of the zebrafish vestibular system. The developmental period identified includes the timeframe during which the vestibular primary afferent neurons are born, innervate their central and peripheral targets, and remodel their central projections. Copyright 2002 Wiley-Liss, Inc.

  20. Navasota Neighborhood Day 

    E-print Network

    Texas Target Communitites

    2014-01-01

    The vision for Navasota Neighborhood Day included a combination tactics and after providing a broad overview, students asked meeting participants to split into smaller breakout groups to discuss the specifics....

  1. Space Day 2000.

    ERIC Educational Resources Information Center

    Winslow, Joyce

    2000-01-01

    Introduces three design challenges for fourth, fifth, and sixth grade students created by the Challenger Center for Space Science Education. Presents information on Space Day and the National Classroom and provides Internet site addresses. (YDS)

  2. Day care health risks

    MedlinePLUS

    ... after going to the bathroom or changing a diaper, and then preparing food. In addition to good ... washing, important policies include: Preparing food and changing diapers in different areas Making sure day care staff ...

  3. STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES

    PubMed Central

    Henle, Werner; Henle, Gertrude; Deinhardt, Friedrich; Bergs, Victor V.

    1959-01-01

    In previous reports of this series, it was shown that persistent infection of MCN cultures with certain myxoviruses rendered the cells insusceptible to superinfection by several cytopathogenic viruses. It was thought that production of an interferon might be the cause of this resistance and efforts to confirm this suggestion have been presented. Addition of ultraviolet-inactivated myxoviruses (mumps, Newcastle disease, influenza A, and Sendai) to MCN cultures for periods of 2 to 3 hours, followed by washing and refeeding of the cells, led to the subsequent release into the media of a substance which induced in fresh MCN cells a transitory resistance to infection by vesicular stomatitis virus, and prevented incomplete reproductive cycles of influenza A and Sendai viruses. Media containing this substance were free of detectable hemagglutinating activity and viral complement-fixing antigens. The substance was not neutralized by specific antiviral sera; it was not sedimentable by high speed centrifugation; it was not adsorbed onto red cells; but it was inactivated by trypsin. Thus, its properties matched those of the interferon described by Isaacs and his associates. A comparison of the extent of resistance induced in MCN cells by decreasing doses of ultraviolet-inactivated myxoviruses (interference test) and the protection afforded by the media removed from the cultures prior to challenge and transferred to fresh MCN tubes (interferon test) revealed that wherever interference became detectable in the cells, the media of the corresponding cultures contained some interferon. Interferon was obtained by inactivated myxoviruses also from primary cell cultures by the same techniques, but not from HeLa cells. Interferons derived from one type of culture may protect others equally well or show a certain degree of host specificity in that resistance in homologous cells may be somewhat more pronounced than in heterologous cultures. No resistance could be induced in HeLa cells by the interferon preparations employed. Interferon was detected also in MCN cultures, persistently infected with mumps virus. Its concentration was apparently too small in carrier cultures maintained as routine to be measurable. However, when the cells were grown in heavy sheets in roller bottles, and especially when the volume of medium was reduced for several days prior to harvest, interferon became readily detectable. These results strengthen the suggestion that interferon may play a decisive role in the establishment and maintenance of persistent infections in the system under study. Its nature, source, mode of action, and exact role in persistent infection remains to be elucidated. PMID:14401038

  4. Stennis Day Camper

    NASA Technical Reports Server (NTRS)

    2005-01-01

    Sara Beth Casey, 5, proudly displays her artwork, 'Planets.' Sara Beth created the art as a student of Stennis Day Camp, a free camp for Stennis Space Center employees' children whose schools have not resumed since Hurricane Katrina hit the region on Aug. 29. The camp has registered nearly 200 children and averages 100 children each day. The camp will continue until all schools are back in session.

  5. Factors Influencing Culture Positivity in Pyogenic Vertebral Osteomyelitis Patients with Prior Antibiotic Exposure

    PubMed Central

    Kim, Chung-Jong; Kang, Seung-Ji; Yoon, Doran; Lee, Myung Jin; Kim, Moonsuk; Song, Kyoung-Ho; Jang, Hee-Chang; Jung, Sook-In; Kim, Eu Suk; Kim, Hong Bin; Oh, Myoung-don

    2015-01-01

    We conducted a retrospective cohort study to evaluate factors influencing tissue culture positivity in patients with pyogenic vertebral osteomyelitis exposed to antibiotics before diagnosis. Tissue culture was positive in 48.3% (28/58) of the patients, and the median antibiotic-free period was 1.5 days (range, 0.7 to 5.7 days). In a multivariate analysis, a higher C-reactive protein (CRP) level (adjusted odds ratio [aOR], 1.18; 95% confidence interval, 1.07 to 1.29) and open surgical biopsy (aOR, 6.33; 95% confidence interval, 1.12 to 35.86) were associated with tissue culture positivity. PMID:25666156

  6. The good old days

    SciTech Connect

    Axford, W.I.

    1994-10-01

    The author touches on personal experiences in the field of space physics during his professional career. The authors career in space physics began at the period of introduction of satellites, and has spanned this period. His training was not in this field, but he was fortunate to be steered into the field at a time of great expansion and significant progress. He was involved in shock wave studies, magnetic reconnection, and a wide range of magnetospheric phenomena.

  7. Development and control of epileptiform bursting in dissociated cortical cultures

    NASA Astrophysics Data System (ADS)

    Wagenaar, Daniel A.

    Cortical cells in dissociated culture form densely interconnected networks. Within days after plating, neurons become electrically active, and soon after start to synchronize their activity into culture-wide bursts. By growing cultures on multi-electrode arrays (Petri dishes with a grid of substrate-embedded electrodes), their electrical activity can be recorded non-invasively.I developed software, MEABench, for online visualization and analysis of multi-electrode data, and used it to follow the development of cultures obtained from (E18) embryonic rats. Globally synchronized bursting was observed in all but the most sparsely plated cultures. A remarkable range of bursting behaviors was observed, even in cultures with identical plating parameters. Activity patterns varied widely in terms of the frequency, intensity, duration, and degree of temporal clustering of bursts. During the 2nd week in vitro, bursts in many dense cultures clustered into well-defined trains, separated by long periods without bursts. The number of bursts within these 'superbursts' and their spatiotemporal structure were found to be stable for hours or days.Cortical cultures on multi-electrode arrays are ideal for studying two-way communication between biological systems and computers. I designed and built hardware to deliver electrical stimuli in arbitrary patterns, developed software to remove stimulation artifacts from recordings, and studied the efficacy of several voltage-defined and current-defined stimulus waveforms. MEABench can control the stimulator in real-time. Thus, stimuli can be made dependent on a culture's activity with only 15 ms lag-time.We hypothesized that synchronized bursting can dominate activity patterns, because lack of external input puts cultures in a hypersensitive state. Indeed, by feeding cultures a steady stream of stimuli, distributed over many electrodes, bursting could be prevented completely. The number of electrodes required for successful burst control could be reduced by fine-tuning the stimuli with real-time feedback, to make each stimulus evoke the same number of spikes. Burst control could not be achieved with single electrode stimulation.For the final chapter, I tested various protocols for inducing plasticity by tetanic stimulation. In contrast to earlier published reports, I found that none of them induced changes in burst patterns or responses to test pulses that exceeded spontaneously occurring changes.

  8. 5 CFR 870.507 - Open enrollment periods.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...insurance elected during an open enrollment period. The...is effective on the 1st day of the first pay period...Within 6 months after an open enrollment period ends...be insured during the open enrollment period. In...an election within 31 days after being...

  9. 5 CFR 870.507 - Open enrollment periods.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...insurance elected during an open enrollment period. The...is effective on the 1st day of the 1st pay period...Within 6 months after an open enrollment period ends...be insured during the open enrollment period. An...be submitted within 60 days after being...

  10. Jupiter Night and Day

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Day and night side narrow angle images taken on January 1, 2001 illustrating storms visible on the day side which are the sources of visible lightning when viewed on the night side. The images have been enhanced in contrast. Note the two day-side occurrences of high clouds, in the upper and lower parts of the image, are coincident with lightning storms seen on the darkside. The storms occur at 34.5 degrees and 23.5 degrees North latitude, within one degree of the latitudes at which similar lightning features were detected by the Galileo spacecraft. The images were taken at different times. The storms' longitudinal separation changes from one image to the next because the winds carrying them blow at different speeds at the two latitudes.

  11. Faculty & Students Houston Day 

    E-print Network

    Unknown

    2011-08-17

    and educational background in order to provide equitable assessment measures (Callahan, 2005; Donovan & Cross, 2009). Many educators are unfamiliar with alternative options for assessing students’ potential for advanced studies (Gardner, 1993; Gay, 2007; Haas... (Anastasi & Urbina, 1997; Callahan, 2005; Ford, 2005; Geisinger, 1992; Harris, et al., 2007; Rhodes, Ochoa, & Ortiz, 2005; Valencia & Suzuki, 2001). Because misunderstandings of culture and language contribute to the underrepresentation of Hispanic...

  12. Subacute (90 days) oral toxicity studies of Kombucha tea.

    PubMed

    Vijayaraghavan, R; Singh, M; Rao, P V; Bhattacharya, R; Kumar, P; Sugendran, K; Kumar, O; Pant, S C; Singh, R

    2000-12-01

    Kombucha tea (KT) is a popular health beverage and is used as an alternative therapy. KT is prepared by placing the kombucha culture in solution of tea and sugar and allowing to ferment. The inoculum is a fungus consisting of symbiotic colony of yeast and bacteria. KT is consumed in several countries and is believed to have prophylactic and therapeutic benefits in a wide variety of ailments, viz., intestinal disorders, arthritis, ageing and stimulation of immunological system. Though KT is used in several parts of the world its beneficial effects and adverse effects have not been scientifically evaluated. Since there are no animal toxicological data on KT, subacute oral toxicity study was carried out. Five groups of rats were maintained: (a) control group given tap water orally, (b) KT given 2 ml/kg orally, (c) plain tea (PT) given 2 ml/kg orally, (d) KT given in drinking water, 1% (v/v) and (e) PT given in drinking water, 1% (v/v). The rats were given this treatment daily for a period of 90 days. Weekly records of weight, feed intake, water intake and general behaviour were monitored. There was no significant difference in the growth of the animals as evidenced by the progressive body weight change. The organ to body weight ratio and histological evaluation did not show any toxic signs. The haematological and biochemical variables were within the clinical limits. The study indicates that rats fed KT for 90 days showed no toxic effects. PMID:11351863

  13. From microcarriers to hydrodynamics: introducing engineering science into animal cell culture.

    PubMed

    Croughan, Matthew S; Hu, Wei-Shou

    2006-10-01

    Professor Daniel I.C. Wang has conducted research in animal cell culture for approximately 40 years. Over that long time period and still to this day, he successfully addresses a multitude of engineering challenges, taking a unique, creative, systems-driven but still fundamental approach. As mammalian cell culture has become the predominant method of manufacturing therapeutic proteins, the impact of his leadership, not only in research but also student recruitment and education, has played a key role in the success of the bio/pharmaceutical industry. PMID:16933297

  14. 38 CFR 3.17 - Disability and death pension; Mexican border period and later war periods.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... pension; Mexican border period and later war periods. 3.17 Section 3.17 Pensions, Bonuses, and Veterans... Compensation General § 3.17 Disability and death pension; Mexican border period and later war periods. In computing the 90 days' service required for pension entitlement (see § 3.3), there will be included...

  15. 38 CFR 3.17 - Disability and death pension; Mexican border period and later war periods.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... pension; Mexican border period and later war periods. 3.17 Section 3.17 Pensions, Bonuses, and Veterans... Compensation General § 3.17 Disability and death pension; Mexican border period and later war periods. In computing the 90 days' service required for pension entitlement (see § 3.3), there will be included...

  16. 38 CFR 3.17 - Disability and death pension; Mexican border period and later war periods.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... pension; Mexican border period and later war periods. 3.17 Section 3.17 Pensions, Bonuses, and Veterans... Compensation General § 3.17 Disability and death pension; Mexican border period and later war periods. In computing the 90 days' service required for pension entitlement (see § 3.3), there will be included...

  17. 38 CFR 3.17 - Disability and death pension; Mexican border period and later war periods.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... pension; Mexican border period and later war periods. 3.17 Section 3.17 Pensions, Bonuses, and Veterans... Compensation General § 3.17 Disability and death pension; Mexican border period and later war periods. In computing the 90 days' service required for pension entitlement (see § 3.3), there will be included...

  18. 38 CFR 3.17 - Disability and death pension; Mexican border period and later war periods.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... pension; Mexican border period and later war periods. 3.17 Section 3.17 Pensions, Bonuses, and Veterans... Compensation General § 3.17 Disability and death pension; Mexican border period and later war periods. In computing the 90 days' service required for pension entitlement (see § 3.3), there will be included...

  19. Tissue-specific Cd and Pb accumulation in Peruvian scallop (Argopecten purpuratus) transplanted to a suspended and bottom culture at Sechura Bay, Peru.

    PubMed

    Loaiza, Iván; Hurtado, Daniela; Miglio, Maria; Orrego, Henry; Mendo, Jaime

    2015-02-28

    In order to understand the effect of different culture systems on Cd and Pb accumulation, suspended long-line and bottom cultures of Argopecten purpuratus were conducted during January until April 2010 (120 days). The Cd tissue levels were the highest at the middle of the experiment (30-d till 70-d) for suspended-cultured individuals, while bottom-cultured individuals showed an increasing trend. Gonad Pb levels were also higher during the same period for all cultures, while adductor muscle exhibited no considerable variations. Cd and Pb tissue concentrations were mainly greater in deeper cultures. There were no significant differences in Cd and Pb accumulation between individual sizes. The Cd and Pb levels in edible tissue (gonad+adductor muscle) did not exceed the EU and FDA maximum levels. Based on the target hazard quotient (THQ) and the provisional tolerance weekly intake (PTWI), no risk (THQ<1 and %PTWI<30) was found for human consumption. PMID:25444617

  20. Melatonin improves neuroplasticity by upregulating the growth-associated protein-43 (GAP-43) and NMDAR postsynaptic density-95 (PSD-95) proteins in cultured neurons exposed to glutamate excitotoxicity and in rats subjected to transient focal cerebral ischemia even during a long-term recovery period.

    PubMed

    Juan, Wei-Sheng; Huang, Sheng-Yang; Chang, Che-Chao; Hung, Yu-Chang; Lin, Yu-Wen; Chen, Tsung-Ying; Lee, Ai-Hua; Lee, Ai-Chiang; Wu, Tian-Shung; Lee, E-Jian

    2014-03-01

    Recent evidence shows that the NMDAR postsynaptic density-95 (PSD-95), growth-associated protein-43 (GAP-43), and matrix metalloproteinase-9 (MMP-9) protein enhance neuroplasticity at the subacute stage of stroke. Here, we evaluated whether melatonin would modulate the PSD-95, GAP-43, and MMP-9 proteins in cultured neurons exposed to glutamate excitotoxicity and in rats subjected to experimental stroke. Adult male Sprague-Dawley rats were treated with melatonin (5 mg/kg) or vehicle at reperfusion onset after transient occlusion of the right middle cerebral artery (tMCAO) for 90 min. Animals were euthanized for Western immunoblot analyses for the PSD-95 and GAP-43 proteins and gelatin zymography for the MMP-9 activity at 7 days postinsult. Another set of animals was sacrificed for histologic and Golgi-Cox-impregnated sections at 28 days postinsult. In cultured neurons exposed to glutamate excitotoxicity, melatonin significantly upregulated the GAP-43 and PSD-95 expressions and improved dendritic aborizations (P<0.05, respectively). Relative to controls, melatonin-treated stroke animals caused a significant improvement in GAP-43 and PSD-95 expressions as well as the MMP-9 activity in the ischemic brain (P<0.05). Consequently, melatonin also significantly promoted the dendritic spine density and reduced infarction in the ischemic brain, and improved neurobehaviors as well at 28 days postinsult (P<0.05, respectively). Together, melatonin upregulates GAP-43, PSD-95, and MMP-9 proteins, which likely accounts for its actions to improve neuroplasticity in cultured neurons exposed to glutamate excitotoxicity and to enhance long-term neuroprotection, neuroplasticity, and brain remodeling in stroke rats. PMID:24350898

  1. Hydroponics or soilless culture

    NASA Technical Reports Server (NTRS)

    Chapman, H. D.

    1963-01-01

    Historically, hydroponics is not a new field; plant physiologists have known and used it for some 100 years. Inevitably, some enthusiasts got carried away.Claims were made of enormous potential yields; skyscraper tops were said to be capable of producing enough food for all of their occupants; and closets, basements, garages, etc. were wishfully converted into fields for hydroponic culture. Numerous publications on the subject appeared during this period. Basic requirements for hydropinc techniques are given along with examples of where soilless culture has been used commercially.

  2. Sun-Earth Day

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Michael Sandras, a member of the Pontchartrain Astronomical Society, explains his solar telescope to students of Second Street in Bay St. Louis, Hancock County and Nicholson elementary schools in StenniSphere's Millennium Hall on April 10. The students participated in several hands-on activities at Stennis Space Center's Sun-Earth Day celebration.

  3. We Love Science Day.

    ERIC Educational Resources Information Center

    Kepler, Lynne

    1986-01-01

    Describes the goals and outcomes of the "We Love Science Day" programs that resulted from the inservice course, "Creative Integration of Science in Elementary Education" for Pennsylvania teachers. Provides samples of the hands-on activities that were offered to students, parents, and teachers. Includes a calendar of extracurricular science…

  4. An Earth Day Reader.

    ERIC Educational Resources Information Center

    Moser, Don, Ed.

    1990-01-01

    Presents what the author believes to be some of the most important environmental books published since Earth Day 1970. Discusses each selection and how it provides the historical background, basic information, and appreciation necessary to understand the character of our environmental dilemma and our need to address it. (MCO)

  5. Volunteer Day Class Photo

    E-print Network

    Sin, Peter

    will walk over together from L/S Classes or LA hours. Please gauge your time to arrive at the Amphitheater will be holding our Thanksgiving Potluck. Details will be on the Activities Board and in the next issue of The ELI Office will be closed on this day. Thanksgiving Holiday: Thursday, November 24th is Thanksgiving

  6. NASA Day of Remembrance

    NASA Technical Reports Server (NTRS)

    2009-01-01

    Stennis Space Center Director Gene Goldman (left) and Deputy Director Patrick Scheuermann place a wreath in StenniSphere in memory of the 17 astronauts lost in service of the space program since 1967. The wreath was placed during NASA's 2009 Day of Remembrance, observed each year on the last Thursday of January.

  7. Day Care: Nutrition.

    ERIC Educational Resources Information Center

    Foster, Florence P.; And Others

    This collection of 12 short, bilingual papers on nutrition and preschool children is part of a series of papers on various aspects of day care published by the Canadian Department of Health and Welfare. Each paper is presented in both English and French. Topics dealt with include an overview of children's nutritional needs; development of…

  8. First Day of School

    ERIC Educational Resources Information Center

    Bort, Nancy

    2004-01-01

    In this brief article, the author, a science teacher at F. C. Hammond Middle School in Alexandria, Virginia, describes how the setting up of a simple science experiment on the first day of school can get students excited about learning science. The experiment involves heating a small amount of water in a flask, then covering the opening of the…

  9. One Play a Day

    ERIC Educational Resources Information Center

    Blankenship, Mark

    2007-01-01

    Undergraduate theater students rarely get the chance to work on a major world premiere, but this year hundreds of them will. Currently, more than 70 colleges and universities are participating in "365 Days/365 Plays," an ambitious project from Pulitzer Prize-winning playwright Suzan-Lori Parks. Every week, as they mount their portion of this epic…

  10. Marketing Your Day Camp.

    ERIC Educational Resources Information Center

    Coleman, George

    1997-01-01

    Marketing strategies for day camps include encouraging camp staff to get involved in organizations involving children, families, and communities; holding camp fairs; offering the use of camp facilities to outside groups; hosting sport leagues and local youth outings; planning community fairs; and otherwise involving the camp in the community. (LP)

  11. Make a Splash Day

    ERIC Educational Resources Information Center

    Coverdale, Greg; Rust, April; Jensen, Belinda

    2004-01-01

    At the annual, all-day events-sponsored by Project WET (Water Education for Teachers) and held in nearly every state across the country each September--students participate in interactive activities and exhibits to learn about water resources and explore how human behaviors, such as development and recreation, can affect the quality of the…

  12. Fabulous Weather Day

    ERIC Educational Resources Information Center

    Marshall, Candice; Mogil, H. Michael

    2007-01-01

    Each year, first graders at Kensington Parkwood Elementary School in Kensington, Maryland, look forward to Fabulous Weather Day. Students learn how meteorologists collect data about the weather, how they study wind, temperature, precipitation, basic types/characteristics of clouds, and how they forecast. The project helps the students grow in…

  13. Dog Day Afternoon.

    ERIC Educational Resources Information Center

    Filipczak, Bob

    1997-01-01

    Discusses the problem faced by trainers who are "on stage" for eight hours a day. Offers tips to relieve the stress caused by continuous training, including maintaining personal space, taking a lunch break, keeping physically energized, and avoiding burnout when teaching the same thing over and over. (JOW)

  14. A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2

    PubMed Central

    Martin, Matthew R.; Fornero, Jeffrey J.; Angenent, Largus T.

    2013-01-01

    We optimized and tested a postbioprocessing step with a single-culture archaeon to upgrade biogas (i.e., increase methane content) from anaerobic digesters via conversion of CO2 into CH4 by feeding H2 gas. We optimized a culture of the thermophilic methanogen Methanothermobacter thermautotrophicus using: (1) a synthetic H2/CO2 mixture; (2) the same mixture with pressurization; (3) a synthetic biogas with different CH4 contents and H2; and (4) an industrial, untreated biogas and H2. A laboratory culture with a robust growth (dry weight of 6.4–7.4?g/L; OD600 of 13.6–15.4), a volumetric methane production rate of 21?L/L culture-day, and a H2 conversion efficiency of 89% was moved to an industrial anaerobic digester facility, where it was restarted and fed untreated biogas with a methane content of ~70% at a rate such that CO2 was in excess of the stoichiometric requirements in relation to H2. Over an 8-day operating period, the dry weight of the culture initially decreased slightly before stabilizing at an elevated level of ~8?g/L to achieve a volumetric methane production rate of 21?L/L culture-day and a H2 conversion efficiency of 62%. While some microbial contamination of the culture was observed via microscopy, it did not affect the methane production rate of the culture. PMID:24194675

  15. July 2007 BEE CULTURE 39 T The Dog Days

    E-print Network

    Delaplane, Keith S.

    usually calls for mint juleps and blackberry pie, cane pole fishing and lemonade, naps in the hammock to cool the interior of the hive. One way they do this is by deposit- ing water directly inside, developing larvae will dry out and die. They also cool the inside by fanning in different areas of the hive

  16. Cosmos: The Integrated Day Comes to College.

    ERIC Educational Resources Information Center

    Kutz, Ronald E.; And Others

    Four full days of classroom instruction, devoted to the modeling of effective curriculum integration, were designed for preservice elementary school teachers. The unit was the result of a conviction on the part of teacher educators that children learn best when learning is not separated into forty-minute periods of math, social studies, language…

  17. Early Adolescence: Celestial Meetings - Synodic Periods.

    ERIC Educational Resources Information Center

    Science and Children, 1985

    1985-01-01

    Rather than memorize numbers for sidereal periods of planets (time to orbit the sun), students can learn to determine these times through simple calculations. Comparison of a planet's synodic period to earth's year of 365 days is made, then multiplication to derive a full circle of 360 degrees establishes the sidereal period. (DH)

  18. When cultures collide.

    PubMed

    Knowles, Thomas W

    2010-01-01

    When it was discovered that the perpetrator of a hit and run accident that sent two severely injured victims to the author's hospital was the father of one of the victims who allegedly tried to kill her for dishonoring the family, a 15 day siege ensued. In the article, the author describes the problems involved in keeping two warring families apart without denigrating their culture and the lessons learned for dealing with such complex situations. PMID:20229935

  19. Day 1 Depart for Italy Day 2 Venice, Italy

    E-print Network

    Toronto, University of

    ITINERARY Day 1 Depart for Italy Day 2 Venice, Italy Day 3 Venice, Italy Day 4 Zadar, Croatia Day 5, tea and juice THE CULINARY CENTER MAY1215 Zadar Venice Kotor Palermo Florence Rome Pisa Barcelona Marseille Monte Carlo Port-Vendres Zadar Venice Kotor Palermo Florence Rome Pisa Barcelona Marseille Monte

  20. The "Little" Cultural Tradition of Hispanics. (La "Pequena" Tradicion Cultural de los Hispanos.)

    ERIC Educational Resources Information Center

    Benitez, Jose Spielberg

    1980-01-01

    Defining "little" cultural traditions as those customs, behaviors and attitudes of ordinary people involved in the day-to-day problems of living and survival, this article traces the origins, historic influences and general characteristics of Hispanic culture and of its Mexican, Puerto Rican and Cuban subcultures. (DS)

  1. Biodegradation of Palm Kernel Cake by Cellulolytic and Hemicellulolytic Bacterial Cultures through Solid State Fermentation

    PubMed Central

    Alshelmani, Mohamed Idris; Loh, Teck Chwen; Foo, Hooi Ling; Lau, Wei Hong; Sazili, Awis Qurni

    2014-01-01

    Four cellulolytic and hemicellulolytic bacterial cultures were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). Two experiments were conducted; the objective of the first experiment was to determine the optimum time period required for solid state fermentation (SSF) of palm kernel cake (PKC), whereas the objective of the second experiment was to investigate the effect of combinations of these cellulolytic and hemicellulolytic bacteria on the nutritive quality of the PKC. In the first experiment, the SSF was lasted for 12 days with inoculum size of 10% (v/w) on different PKC to moisture ratios. In the second experiment, fifteen combinations were created among the four microbes with one untreated PKC as a control. The SSF lasted for 9 days, and the samples were autoclaved, dried, and analyzed for proximate analysis. Results showed that bacterial cultures produced high enzymes activities at the 4th day of SSF, whereas their abilities to produce enzymes tended to be decreased to reach zero at the 8th day of SSF. Findings in the second experiment showed that hemicellulose and cellulose was significantly (P < 0.05) decreased, whereas the amount of reducing sugars were significantly (P < 0.05) increased in the fermented PKC (FPKC) compared with untreated PKC. PMID:25019097

  2. Day One Sustainability

    ERIC Educational Resources Information Center

    Orr, John; Ibell, Timothy; Evernden, Mark; Darby, Antony

    2015-01-01

    Emissions reductions targets for the UK set out in the Climate Change Act for the period to 2050 will only be achieved with significant changes to the built environment, which is currently estimated to account for 50% of the UK's carbon emissions. The socio-technological nature of Civil Engineering means that this field is uniquely placed to lead…

  3. Ryukyuan Culture.

    ERIC Educational Resources Information Center

    Trafton, Terry

    The Ryukyu Islands of Japan, of which Okinawa is the best known, possess a lengthy history and a sophisticated cultural background, an exploration of which helps to shed light on this area and on mainland Japan. This document is an exposition of Ryukuan culture. Divided into eight sections, the areas covered include: (1) Historical perspective;…

  4. Pop Culture.

    ERIC Educational Resources Information Center

    Berger, Arthur Asa

    Popular culture in America is comprehensively reviewed in this book. The book is designed for either high school or college level social studies or English courses and includes a variety of graphic illustrations that provide a thread of continuity throughout. Basically the author has developed a method of analysis that reveals how popular culture

  5. Cultural Communications.

    ERIC Educational Resources Information Center

    Armas, Jose

    It is too often taken for granted that the communication process with culturally different children takes place as readily as it might with children from Anglo cultures. Most teachers receive training in verbal and formal communication skills; children come to school with nonverbal and informal communication skills. This initially can create…

  6. Good Meals Every Day

    E-print Network

    Cox, Maeona; Reasonover, Frances; Harris, Jimmie Nell; Mason, Louise

    1955-01-01

    , flu IP okra 1 I- Green asparagus 1 Eggplant (van Raw, cooked, ( frozen or er canned-]: CUP or nlor each day I Green snap beans Broccoli Brussel sprouts Green cabbage Chard Collards Kale Leaf lettuce Mustard greens Green okra Green... Custard Milk Supper Steak Sandwiches (Made from leftover Swiss steak) Stewed Tomatoes Buttered Okra Whole Wheat Bread Butter or Margarine Frozen Custard WEDNESDAY FRIDAY I I Dinner Dinner Meat Loaf Tomato Sauce - String Beans Buttered Beets...

  7. Flight Day 2 Highlights

    NASA Technical Reports Server (NTRS)

    2003-01-01

    The STS-107 second flight day begins with a shot of the Spacehab Research Double Module. Live presentations of experiments underway inside of the Spacehab Module are presented. Six experiments are shown. As part of the Space Technology and Research Student Payload, students from Australia, China, Israel, Japan, New York, and Liechtenstein are studying the effect that microgravity has on ants, spiders, silkworms, fish, bees, granular materials, and crystals. Mission Specialist Kalpana Chawla is seen working with the zeolite crystal growth experiment.

  8. Effect of Matrix on Cardiomyocyte Viscoelastic Properties in 2D Culture

    PubMed Central

    Deitch, Sandra; Gao, Bruce Z.; Dean, Delphine

    2012-01-01

    Cardiomyocyte phenotype changes significantly in 2D culture systems depending on the substrate composition and organization. Given the variety of substrates that are used both for basic cardiac cell culture studies and for regenerative medicine applications, there is a critical need to understand how the different matrices influence cardiac cell mechanics. In the current study, the mechanical properties of neonatal rat cardiomyocytes cultured in a subconfluent layer upon aligned and unaligned collagen and fibronectin matrices were assessed over a two week period using atomic force microscopy. The elastic modulus was estimated by fitting the Hertz model to force curve data and the percent relaxation was determined from stress relaxation curves. The Quasilinear Viscoelastic (QLV) and Standard Linear Solid (SLS) models were fit to the stress relaxation data. Cardiomyocyte cellular mechanical properties were found to be highly dependent on matrix composition and organization as well as time in culture. It was observed that the cells stiffened and relaxed less over the first 3 to 5 days in culture before reaching a plateau in their mechanical properties. After day 5, cells on aligned matrices were stiffer than cells on unaligned matrices and cells on fibronectin matrices were stiffer than cells on collagen matrices. No such significant trends in percent relaxation measurements were observed but the QLV model fit the data very well. These results were correlated with observed changes in cellular structure associated with culture on the different substrates and analyzed for cell-to-cell variability. PMID:23285736

  9. Enhanced Biosynthesis of Withanolides by Elicitation and Precursor Feeding in Cell Suspension Culture of Withania somnifera (L.) Dunal in Shake-Flask Culture and Bioreactor

    PubMed Central

    Sivanandhan, Ganeshan; Selvaraj, Natesan; Ganapathi, Andy; Manickavasagam, Markandan

    2014-01-01

    The present study investigated the biosynthesis of major and minor withanolides of Withania somnifera in cell suspension culture using shake-flask culture and bioreactor by exploiting elicitation and precursor feeding strategies. Elicitors like cadmium chloride, aluminium chloride and chitosan, precursors such as cholesterol, mevalonic acid and squalene were examined. Maximum total withanolides detected [withanolide A (7606.75 mg), withanolide B (4826.05 mg), withaferin A (3732.81 mg), withanone (6538.65 mg), 12 deoxy withanstramonolide (3176.63 mg), withanoside IV (2623.21 mg) and withanoside V (2861.18 mg)] were achieved in the combined treatment of chitosan (100 mg/l) and squalene (6 mM) along with 1 mg/l picloram, 0.5 mg/l KN, 200 mg/l L-glutamine and 5% sucrose in culture at 4 h and 48 h exposure times respectively on 28th day of culture in bioreactor. We obtained higher concentrations of total withanolides in shake-flask culture (2.13-fold) as well as bioreactor (1.66-fold) when compared to control treatments. This optimized protocol can be utilized for commercial level production of withanolides from suspension culture using industrial bioreactors in a short culture period. PMID:25089711

  10. Perspectives on Infant Day Care.

    ERIC Educational Resources Information Center

    Elardo, Richard, Ed.; Pagan, Betty, Ed.

    This second edition contains articles on (1) infant day care, (2) day care as a way to extend parental support systems, (3) meeting developmental needs of infants, (4) ecology of day care, (5) ecology of infant day care, (6) quality care for infants, (7) the daily schedule, (8) precautions in establishing infant day care, (9) teaching--learning…

  11. THE SUSCEPTIBILITY OF CHICK EMBRYO SKIN ORGAN CULTURES TO INFLUENZA VIRUS FOLLOWING EXCESS VITAMIN A

    PubMed Central

    Huang, J. S.; Bang, F. B.

    1964-01-01

    The conversion of chick embryonic epidermis to mucous epithelium by excess vitamin A in organ culture as reported by Fell and Mellanby (5) was shown to be accompanied by a corresponding change of susceptibility to influenza and vaccinia viruses. Untreated epidermis of 10- to 12-day chick embryos supported the growth of influenza (PR8) virus in organ cultures and a maximum infectivity (EID50) titer was reached 2 to 3 days after infection. At the same time) the epidermis showed squamous keratinization, beginning about the 4th day of cultivation. Addition of excess vitamin A (40 µg per ml) to the skin organ culture induced the following changes: (a) mucous metaplasia of the epidermis which was usually first evident after 4 to 5 days in the vitamin A medium, (b) increase in the daily and maximum yield of influenza virus, if the epidermis had been grown for 4 or more days in the vitamin A medium before infection took place, and (c) decrease in the production of vaccinia virus under similar conditions. The maximum yield of both viruses remained unchanged, however, if excess vitamin A was introduced to the organ culture at the time of virus inoculation. The magnitude of increase in the yield of influenza virus in this organ culture system was found to be proportionally related to the concentration of vitamin A added 4 or more days before inoculation of this virus. Increasing doses of vitamin A however, had no effect on the short-term growth of influenza virus in tissue cultures of chorio-allantoic membrane. Observation on the early period (2 to 12 hours) of influenza virus growth initiated in the 4-day organ cultures of chick embryonic skin showed no significant difference in virus production between the normal and the vitamin A medium groups. The change of virus specificity apparently is not due to the presence of excess vitamin A per se, but appears to be related to the change of differentiation produced in the organ culture system. PMID:14206436

  12. National Adult Day Services Association

    MedlinePLUS

    ... near you THE NATIONAL VOICE FOR THE ADULT DAY SERVICE COMMUNITY The National Adult Day Services Association is seeking an Executive Director. Click ... the national development, recognition and use of adult day services. They provide a coordinated program of professional ...

  13. One Cold Autumn Day

    PubMed Central

    de Schweinitz, Peter

    2015-01-01

    Behavioral change is at the heart of effective primary care, but when patients don’t change, how do we account for our days? In this personal essay, I relate an encounter with a patient who wants to quit smoking, lose weight, and control her diabetes. I am discouraged when she deflects my recommendations, but a colleague’s comment encourages a deeper inquiry. Knowing the patient’s story and deepening the conversation, however, do not guarantee change. The experience reminds me why patience, humility, and faith are core values of the primary care physician. PMID:25964410

  14. 2010 Day of Remembrance

    NASA Technical Reports Server (NTRS)

    2010-01-01

    Patrick Scheuermann (left), deputy director at NASA's John C. Stennis Space Center, and Richard Gilbrech, associate director, place a wreath in memory of the 17 astronauts lost in service of the space program since 1967. The wreath was placed during NASA's 2010 Day of Remembrance, observed each year in January. The annual observance memorializes the three astronauts lost in the Apollo 1 launch pad fire in 1967, the seven astronauts lost in the Challenger tragedy in 1986 and the seven astronauts lost in the Columbia accident in 2003. During the Stennis observance, Scheuermann praised the fallen astronauts as 'brave space pioneers who gave their lives in the cause of exploration and discovery.'

  15. World AIDS Day 

    E-print Network

    Valentine, A.

    2000-01-01

    -based identity constructions in the blue-collar workplace. 9 Data for the study comes from a larger corpus of audiovisual data gathered over two days of work in a Houston-area restaurant kitchen, Shadow.3 My research venue was selected because... Dale, is a white man in his thirties; and Alina, a heavy-set African American line cook who works alongside of Phil, the lowest-ranking male cook present during data collection. Raw data was transcribed with Transana 2.41 software using Jeffersonian...

  16. Effect of delays in processing on the survival of Mycobacterium avium-M. intracellulare in the isolator blood culture system.

    PubMed Central

    von Reyn, C F; Hennigan, S; Niemczyk, S; Jacobs, N J

    1991-01-01

    Concentrations of Mycobacterium avium-M. intracellulare ranging from 10(-1) to 10(3) CFU/ml were added to blood, placed in Isolator tubes, and held at room temperature for intervals ranging from 4 h to 56 days before being processed (centrifugation and culture on Middlebrook 7H10 agar). At all concentrations tested, M. avium-M. intracellulare was recovered after hold times ranging from 4 h to 7 days; the number of final CFU actually increased progressively for hold times of 8 h or more. Hold times of up to 7 days did not increase the time from processing to the first appearance of visible colonies. At an inoculum of 10(2) CFU/ml, M. avium-M. intracellulare was recovered from Isolator tubes processed 56 days after inoculation. Two Isolator blood cultures were drawn from a patient with AIDS; M. avium-M. intracellulare was recovered from the sample processed immediately and from the sample processed after a hold time of 7 days. Since M. avium-M. intracellulare survives for prolonged periods in Isolator tubes, blood cultures may be collected in outpatient settings or in hospitals without mycobacterial culture facilities and shipped to reference laboratories for processing without loss of viability. PMID:1864940

  17. Biosynthesis of titin in cultured skeletal muscle cells

    SciTech Connect

    Isaacs, W.B.; Kim, I.S.; Struve, A.; Fulton, A.B. )

    1989-11-01

    Although significant progress has been made regarding the structure and function of titin, little data exist on the biosynthesis of this large protein in developing muscle. Using pulse-labeling with ({sup 35}S)methionine and immunoprecipitation with an anti-titin mAb, we have examined the biosynthesis of titin in synchronized cultures of skeletal muscle cells derived from day 12 chicken embryos. We find that: (a) titin synthesis increases greater than 4-fold during the first week in culture and during this same time period, synthesis of muscle-specific myosin heavy chain increases greater than 12-fold; (b) newly synthesized titin has a t1/2 of approximately 70 h; (c) titin is resistant to extraction with Triton X-100 both during and immediately after its synthesis. These observations suggest that newly synthesized titin molecules are stable proteins that rapidly associate with the cytoskeleton of developing myotubes.

  18. Polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique.

    PubMed

    Mukherjee, A; Pramanik, S; Das, D; Roy, R; Therese, K L

    2014-01-01

    Accurate etiological diagnosis is the key to prevention of ocular morbidity in endophthalmitis cases. A 66 year old male was suffering from chronic endophthalmitis post-cataract surgery. Polymerase chain reaction examination on anterior chamber fluid was positive for Propionibacterium acnes but negative for the panfungal genome. He was advised vitrectomy with intravitreal injections. Polymerase chain reaction of vitreous aspirate was positive for P.acnes as well as panfungal genome. The vitreous sample also grew yeast in culture which was identified as Candida pseudotropicalis. Patient was treated on an alternate day regimen of intravitreal Vancomycin and Amphotericin B in the post-operative period. There was improvement in vision at final follow up. Chronic endophthalmitis can have polymicrobial etiology which will require appropriate diagnostic and therapeutic strategies. The role of molecular testing is vital in these cases as growth in culture is often negative. PMID:25008833

  19. Circumstellar Quasi-periods Accompanying Stellar Periods of Be Stars

    NASA Astrophysics Data System (ADS)

    Stefl, S.; Baade, D.; Rivinius, Th.; Stahl, O.; Wolf, B.; Kaufer, A.

    In 1995, 1996, and 1997, several hundred high-resolving power (20,000) echelle spectra (345-862 nm) were for each of the bright southern Be stars 28 CMa, eta Cen and mu Cen obtained with the Heidelberg fiber-coupled echelle spectrograph (HEROS). The results of a Fourier analysis, using also the CLEAN algorithm, of typically 30 spectral lines show that the character of the periodicity probably depends on the site of formation of the lines. Two basic periods are present in each star which differ by typically 0.1 day and can be resolved only in sufficiently long observing series. The main periods, which agree with those detected by previous observations, appear in most lines with constant power distribution across the profiles. The properties of the secondary periods seem to be modified by the size of the envelope as deduced from the width of the rotationally broadened emission lines or, in mu Cen, by variations in the emission strength. The presence and properties of such a `satellite period' can be best demonstrated for 28 CMa. The long-known 1.37-day period dominates in photospheric lines of He I, Si III and Ne I as well as in the cores of most of the other stellar lines. The second period (1.47 days) prevails in the emission lines of the Paschen series and O I and is present also in the wings of the photospheric lines. Both variabilities are of comparable power in the lines of H I, Si II, and Mg II. Possible explanations of and relations between the two variabilities will also be presented.

  20. Three-day fever.

    PubMed

    Akakpo, A J

    2015-08-01

    Three-day fever is a viral disease caused by an Ephemerovirus of the family Rhabdoviridae, transmitted by arthropod vectors. It is common in tropical and sub-tropical regions, where it affects mainly domestic cattle and buffaloes, especially in intensive dairy or fattening production systems. It is of economic importance because it reduces milk production and fertility and causes abortion. The disease is generally benign. It manifests in several susceptible subjects simultaneously, with a sudden episode of fever accompanied by muscle involvement with arthritis, stiffness of the limbs, and lameness, followed by rapid recovery. The presence of a serofibrinous exudate in the joints is indicative of the disease. Clinical diagnosis is often difficult in the absence of pathognomonic signs. Epidemiological factors (proliferation of arthropod vectors), associated with a short-lived fever and the presence of many immature neutrophils, point strongly to three-day fever. In the absence of any specific treatment, the symptoms are treated with antibiotics and anti-inflammatories. Medical prophylaxis currently uses live attenuated vaccines, pending the development of recombinant vaccines, which are giving promising results. PMID:26601454

  1. Induction and Analysis of the Alkaloid Mitragynine Content of a Mitragyna speciosa Suspension Culture System upon Elicitation and Precursor Feeding

    PubMed Central

    Mohamad Zuldin, Nor Nahazima; Said, Ikram Md.; Mohd Noor, Normah; Zainal, Zamri; Jin Kiat, Chew; Ismail, Ismanizan

    2013-01-01

    This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in a Mitragyna speciosa suspension culture. The best callus induction was achieved from petiole explants cultured on WPM that was supplemented with 4?mg?L?1 2, 4-D (70.83%). Calli were transferred to liquid media and agitated on rotary shakers to establish Mitragyna speciosa cell suspension cultures. The optimum settled cell volume was achieved in the presence of WPM that contained 3?mg?L?1 2,4-D and 3% sucrose (9.47 ± 0.4667?mL). The treatment of cultures with different concentrations of yeast extract and salicylic acid for different inoculation periods revealed that the highest mitragynine content as determined by HPLC was achieved from the culture treated with 250?mg?L?1 yeast extract (9.275 ± 0.082?mg?L?1) that was harvested on day 6 of culturing; salicylic acid showed low mitragynine content in all concentrations used. Tryptophan and loganin were used as exogenous precursors; the highest level of mitragynine production was achieved in cultures treated with 3??M tryptophan and harvested at 6 days (13.226 ± 1.98?mg?L?1). PMID:24065873

  2. Three Dimensional Primary Hepatocyte Culture

    NASA Technical Reports Server (NTRS)

    Yoffe, Boris

    1998-01-01

    Our results demonstrated for the first time the feasibility of culturing PHH in microgravity bioreactors that exceeded the longest period obtained using other methods. Within the first week of culture, isolated hepatocytes started to form aggregates, which continuously increased in size (up to 1 cm) and macroscopically appeared as a multidimensional tissue-like assembly. To improve oxygenation and nutrition within the spheroids we performed experiments with the biodegradable nonwoven fiber-based polymers made from PolyGlycolic Acid (PGA). It has been shown that PGA scaffolds stimulate isolated cells to regenerate tissue with defined sizes and shapes and are currently being studied for various tissue-engineering applications. Our data demonstrated that culturing hepatocytes in the presence of PGA scaffolds resulted in more efficient cell assembly and formations of larger cell spheroids (up to 3 cm in length, see figure). The histology of cell aggregates cultured with PGA showed polymer fibers with attached hepatocytes. We initiated experiments to co-culture primary human hepatocytes with human microvascular endothelial cells in the bioreactor. The presence of endothelial cells in co-cultures were established by immunohistochemistry using anti-CD34 monoclonal Ab. Our preliminary data demonstrated that cultures of purified hepatocytes with human microvascular endothelial cells exhibited better growth and expressed higher levels of albumin MRNA for a longer period of time than cultures of ppfified, primary human hepatocytes cultured alone. We also evaluated microsomal deethylation activity of hepatocytes cultured in the presence of endothelial cells.In summary, we have established liver cell culture, which mimicked the structure and function of the parent tissue.

  3. Day 1 Depart for Peru Day 2 Lima (Callao), Peru

    E-print Network

    Toronto, University of

    Fjords Day 13 Cruising the Chilean Fjords Day 14 Punta Arenas, Chile Day 15 Ushuaia, Argentina Day 16 FJORDS 21-NIGHT LUXURY CRUISE ABOARD REGATTA · LIMA to BUENOS AIRES FEBRUARY 6­28, 2016 WHY TRAVELERS. FEB1615 Alluring Andes and the Majestic Fjords: 02/06/16 ­ 02/28/16 Lima Pisco Matarani

  4. Urine culture

    MedlinePLUS

    Culture and sensitivity - urine ... sample will be collected as a clean catch urine sample in your health care provider's office or ... will use a special kit to collect the urine. A urine sample can also be taken by ...

  5. Blood culture

    MedlinePLUS

    Culture - blood ... A blood sample is needed. The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  6. Esophageal culture

    MedlinePLUS

    ... lab. There, it is placed in a special dish (culture) and watched for the growth of bacteria, ... means that no germs grew in the laboratory dish. Normal value ranges may vary slightly among different ...

  7. Bile culture

    MedlinePLUS

    ... lab. There, it is placed in a special dish called a culture medium to see if bacteria, ... bacteria, virus, or fungus grew in the laboratory dish. Note: Normal value ranges may vary slightly among ...

  8. Developing Multicultural Awareness: An In-service Day Proposal for Rend Lake College, Ina, Illinois.

    ERIC Educational Resources Information Center

    Rust, Joseph Henry

    Noting that the state of Illinois mandates that community colleges address cultural diversity in their curriculum, this paper presents a proposal for a day-long in-service faculty education program to make faculty aware that cultural diversity exists at Rend Lake College (Illinois). The paper begins with a schedule for the in-service day, offering…

  9. Proceedings, Dean's Day 1999

    SciTech Connect

    Zanner, M.A.

    1999-03-01

    On January 14--15, 1999, Sandia National Laboratories sponsored Deans Day, a conference for the Deans of Engineering and other executive-level representatives from 29 invited universities. Through breakout sessions and a wrap-up discussion, university and Sandia participants identified activities to further develop their strategic relationships. The four primary activities are: (A) concentrate joint efforts on current and future research strengths and needs; (B) attract the best students (at all grade levels) to science and engineering; (C) promote awareness of the need for and work together to influence a national science and technology R and D policy; and (D) enable the universities and Sandia to be true allies, jointly pursuing research opportunities and funding from government agencies and industry.

  10. World HABITAT Day message.

    PubMed

    Ramachandran, A

    1990-01-01

    The Executive Director of the UN Centre for Human Settlements (HABITAT) discusses the 1989 World HABITAT Day message--Shelter, Health, and the Family. Women and children spend most of their time in their homes and neighborhoods. The environment and the way they live in that environment determine their health status and well being. Healthy housing encompasses protection against rain, heat, cold, and disease. Yet more than 25% of the world's population have inadequate housing which is responsible for various communicable and chronic diseases, injuries, and psychological stresses. For example, overcrowding leads to respiratory infection. Inferior building materials and designs foster the harboring and breeding of disease vectors and destruction by natural disasters. Limited access to potable water and sanitation facilities and poor personal hygiene cause death, disabilities, and blemishes to millions of people as well as fosters food contamination. 10,000 people die each day from injuries or conditions directly related to inadequate shelter and related services. The urban poor tend to be at the point where industrialization meets underdevelopment because they live in marginal areas often near refuse dumps, swamps, and areas subject to landslides, earthquake, or flooding. Social and psychological problems also emerge from such an environment which further undermine the underpinnings of a secure and pleasant family life. Few activities designed to improve shelter consider the health of the occupants, however. Education about the link between housing and health among the poor at the household and community levels may empower them to alleviate the health hazards. The Executive Director requests the international community, governments, their agencies, nongovernmental organizations, builders, planners, and policymakers to consider the link between housing and health when making policy and decisions. PMID:2326215

  11. A cultural, customizable and prefabricated housing grammar for Casablanca

    E-print Network

    Akkar, Ghita

    2011-01-01

    Proposing an innovative design grammar linking prefabrication, customization and cultural adaptability, this thesis addresses the present day housing deficit and lack of architectural identity in Casablanca, Morocco. The ...

  12. Temperature responsive hydrogels enable transient three-dimensional tumor cultures via rapid cell recovery.

    PubMed

    Heffernan, John M; Overstreet, Derek J; Srinivasan, Sanjay; Le, Long D; Vernon, Brent L; Sirianni, Rachael W

    2016-01-01

    Recovery of live cells from three-dimensional (3D) culture would improve analysis of cell behaviors in tissue engineered microenvironments. In this work, we developed a temperature responsive hydrogel to enable transient 3D culture of human glioblastoma (GBM) cells. N-isopropylacrylamide was copolymerized with hydrophilic grafts and functionalized with the cell adhesion peptide RGD to yield the novel copolymer poly(N-isopropylacrylamide-co-Jeffamine(®) M-1000 acrylamide-co-hydroxyethylmethacrylate-RGD), or PNJ-RGD. This copolymer reversibly gels in aqueous solutions when heated under normal cell culture conditions (37°C). Moreover, these gels redissolve within 70 s when cooled to room temperature without the addition of any agents to degrade the synthetic scaffold, thereby enabling rapid recollection of viable cells after 3D culture. We tested the efficiency of cell recovery following extended 3D culture and were able to recover more than 50% of viable GBM cells after up to 7 days in culture. These data demonstrate the utility of physically crosslinked PNJ-RGD hydrogels as a platform for culture and recollection of cells in 3D. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 17-25, 2016. PMID:26123863

  13. Reconstruction of conjunctival epithelium-like tissue using a temperature-responsive culture dish

    PubMed Central

    Yao, Qinke; Zhu, Mengyu; Chen, Junzhao; Shao, Chunyi; Yan, Chenxi; Wang, Zi; Fan, Xianqun; Gu, Ping

    2015-01-01

    Purpose To study the feasibility of engineering conjunctival epithelial cell sheets on a temperature-responsive culture dish for ocular surface reconstruction. Methods Rabbit conjunctival epithelial cells (rCjECs) were cultured in DMEM/F-12 (1:1) medium. The morphology and phenotype of the rCjECs were confirmed with phalloidin staining, periodic acid–Schiff (PAS) staining, and immunocytochemistry. The rCjECs cultured on a temperature-responsive culture dish for 10 days produced confluent conjunctival epithelial cell sheets. Then, the phenotype, structure, and function of the conjunctival epithelial cell sheets were examined. Results The conjunctival epithelial cells were compact, uniform, and cobblestone shape. All cultured conjunctival epithelial cells were harvested as intact cell sheets by reducing the culture temperature to 20 °C. Conjunctival epithelial cells were stratified in four to five cell layers similar to the conjunctival epithelium. CCK-8 analysis, 5-bromo-2’-deoxyuridine (BrdU) staining, and the live and dead viability assay confirmed that viable proliferation cells were retained in the cell sheets. Immunohistochemistry for CK4, CK19, and MUC5AC showed the cell sheets still maintained characteristics of the conjunctival epithelium. Conclusions A temperature-responsive culture dish enables fabrication of viable conjunctival epithelial cell sheets with goblet cells and proliferative cells. Conjunctival epithelial cell sheets will be promising for reconstruction of the conjunctival epithelium. PMID:26396489

  14. [Day Care in Canada - 1982.

    ERIC Educational Resources Information Center

    Department of National Health and Welfare, Ottawa (Ontario). National Day Care Information Center.

    The three reports collected in this document focus on day care in the Canadian provinces, specifically addressing: (1) "Provincial Funding of Day Care Services"; (2) "Day Care Spaces in Canada - 1982"; and (3) "Provincial Day Care Requirements." The first report provides summary outlines of the mechanisms used by each province or territory to…

  15. Family Day Care Training Curriculum.

    ERIC Educational Resources Information Center

    Nakatsu, Gail

    California's Family Day Care Training Program was designed to recruit and train in 7 weeks, Lao, Vietnamese, and Chinese refugees to establish their own state-licensed, family day care homes. Topics in the program's curriculum include an introduction to family day care, state licenses for family day care, state licensing requirements for family…

  16. Culturally Responsive Teaching: Understanding Disability Culture

    ERIC Educational Resources Information Center

    Darrow, Alice-Ann

    2013-01-01

    To be culturally responsive teachers, we must first have an understanding of other cultures and how students from these cultures differ from one another. As we consider the many cultures represented in our classrooms, we might also consider students with disabilities as a cultural group. Within any main culture are subgroups differentiated by…

  17. Spatial Information in local society's cultural conservation and research

    NASA Astrophysics Data System (ADS)

    Jang, J.-J.; Liao, H.-M.; Fan, I.-C.

    2015-09-01

    Center for Geographic Information Science, Research Center for Humanities and Social Sciences,Academia Sinica (GIS center), Coordinate short-, medium-, and long-term operations of multidisciplinary researches focusing on related topics in the sciences and humanities. Based on the requirements of multi-disciplinary research applications, sustain collection and construction of sustaining and unifying spatial base data and knowledge and building of spatial data infrastructure. Since the 1990s, GIS center build geographic information platform: "Time and space infrastructure of Chinese civilization" (Chinese Civilizationin Time and Space, CCTS) and "Taiwan History and Culture Map" (Taiwan History and Culture in Time and Space, THCTS) . the goal of both system is constructing an integrated GIS-based application infrastructure on the spatial extent of China and Taiwan, in the timeframe of Chinese and Taiwanese history, and with the contents of Chinese and Taiwanese civilization. Base on THCTS, we began to build Cultural Resources GIS(CRGIS, http://crgis.rchss.sinica.edu.tw) in 2006, to collect temples, historic Monuments, historic buildings, old trees, wind lions god and other cultural resource in Taiwan, and provide a platform for the volunteers to make for all types of tangible, intangible cultural resources, add, edit, organize and query data via Content Management System(CMS) . CRGIS collected aggregated 13,000 temples, 4,900 churches. On this basis, draw a variety of religious beliefs map-multiple times Temple distributions, different main god distributions, church distribution. Such as Mazu maps, Multiple times temple distributions map (before 1823, 1823-1895,1895-1949,1949-2015 years) at Taijiang inner sea areas in Tainan. In Taiwan, there is a religious ritual through folk activities for a period ranging from one day to several days, passing specific geospatial range and passes through some temples or houses. Such an important folk activity somewhat similar to Western parade, called " raojing " , the main spirit is passing through of these ranges in the process, to reach the people within bless range, many scholars and academic experts's folk research are dependent on such spatial information. 2012, GIS center applied WebGIS and GPS to gather raojing activities spatial information in cooperation with multi-units, aggregated seven sessions, 22 days, 442 temples had pass through . The atlas also published named "Atlas of the 2012 Religious Processions in the Tainan Region" in 2014. we also applied national cultural resources data form relevant government authorities, through the metadata design and data processing(geocoding), established cultural geospatial and thematic information ,such as 800 monuments, 1,100 historic buildings and 4,300 old trees data. In recent years, based on CRGIS technology and operational concepts, different domain experts or local culture-ahistory research worker/team had to cooperate with us to establish local or thematic material and cultural resources. As in collaboration with local culture-history research worker in Kinmen County in 2012, build Kinmen intangible cultural assets - Wind Lion God ,set metadata and build 122 wind lion god `s attribute data and maps through field survey, it is worth mention such fieldwork data integrity is more than the official registration data form Kinmen National Park, the number of is wind lion god more than 40; in 2013,we were in cooperation with academic experts to establish property data and map of the theatre during the Japanese colonial era in Taiwan, a total of 170 theatres ; we were in cooperation with Japanese scholars, used his 44 detaile field survey data of sugar refineries during the Japanese colonial era in Taiwan ,to produce a sugar refineries distribution map and extend to a thematic web(http://map.net.tw/) [The Cultural Heritage Maps of Taiwan Suger Factories in a Century]site according to CRGIS independent cultural concept. Deployment and operation of the CRGIS, the meaning is not only build the themati

  18. TRADURRE IN ITALIANO IL TESTO SEGUENTE: The approximate number of days in a year is 365.2422, equal to 365 days 5 hours

    E-print Network

    Robbiano, Lorenzo

    TRADURRE IN ITALIANO IL TESTO SEGUENTE: Calendar The approximate number of days in a year is 365 times the period of the Moon. In the Julian calendar the ordinary years have 365 days, but every year. The average Julian year has therefore 365.25 days and is one day out approximately every 128 years

  19. 7 CFR 247.16 - Certification period.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE CHILD NUTRITION PROGRAMS COMMODITY SUPPLEMENTAL FOOD PROGRAM § 247.16 Certification period. (a... final day of the month in which eligibility expires (e.g., the last day of the month in which a child... discrimination by race, color, national origin, age, sex, or disability. (Approved by the Office of...

  20. 7 CFR 247.16 - Certification period.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE CHILD NUTRITION PROGRAMS COMMODITY SUPPLEMENTAL FOOD PROGRAM § 247.16 Certification period. (a... final day of the month in which eligibility expires (e.g., the last day of the month in which a child... discrimination by race, color, national origin, age, sex, or disability. (Approved by the Office of...

  1. 7 CFR 247.16 - Certification period.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE CHILD NUTRITION PROGRAMS COMMODITY SUPPLEMENTAL FOOD PROGRAM § 247.16 Certification period. (a... final day of the month in which eligibility expires (e.g., the last day of the month in which a child... discrimination by race, color, national origin, age, sex, or disability. (Approved by the Office of...

  2. 39 CFR 121.2 - Periodicals.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... applied to Periodicals pieces properly accepted before the day-zero Critical Entry Time (CET) and merged... pieces properly accepted before the day-zero CET and merged with First-Class Mail pieces for surface... contiguous 48 states, they are not merged with First-Class Mail pieces for surface transportation (as per...

  3. 39 CFR 121.2 - Periodicals.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... applied to Periodicals pieces properly accepted before the day-zero Critical Entry Time (CET) and merged... pieces properly accepted before the day-zero CET and merged with First-Class Mail pieces for surface... contiguous 48 states, they are not merged with First-Class Mail pieces for surface transportation (as per...

  4. 39 CFR 121.2 - Periodicals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... properly accepted before the day-zero Critical Entry Time (CET) and merged with First-Class Mail pieces for... standard is applied to Periodicals pieces properly accepted before the day-zero CET and merged with First... originate and destinate within the contiguous 48 states, they are not merged with First-Class Mail...

  5. Detection of mumps virus in clinical specimens by rapid centrifugation culture and conventional tube cell culture.

    PubMed

    Germann, D; Gorgievski, M; Ströhle, A; Matter, L

    1998-07-01

    Conventional tube cell culture was compared with a 2 day and 5 day spin-amplified shell vial indirect immunofluorescence assay for the detection of mumps virus in swabs from the area of Stensen's duct. The sensitivity and specificity of the shell vial assay were 95.9 and 100%, respectively. The shell vial detected 66.3% of the positive cultures within 2 days of inoculation while the first positive results were available by conventional tube cell culture after 3 days (1.6%) reaching 72.4% of all culture positive specimens after 7 days. These data suggest that a centrifugation shell vial indirect immunofluorescence assay may be useful for rapid detection of mumps virus in clinical specimens. PMID:9705175

  6. Safety of high doses of Propionibacterium freudenreichii ET-3 culture in healthy adult subjects.

    PubMed

    Uchida, Masayuki; Tsuboi, Hiroshi; Takahashi Arita, Masayo; Nemoto, Atsushi; Seki, Keisuke; Tsunoo, Hajime; Martyres, Stephanie; Roberts, Ashley

    2011-07-01

    Propionibacterium freudenreichii ET-3 (7025) culture, a cell-free product of whey fermentation by P. freudenreichii ET-3, has been shown to promote the growth of Bifidobacteria through the action of 1,4-dihydroxy-2-naphthoic acid (DHNA). Here we report the results of two clinical studies designed to evaluate the safety of high doses of P. freudenreichii ET-3 culture medium. Study 1 had a randomized, double-blind, crossover design. Ten healthy male and four healthy female subjects received 45 tablets of either P. freudenreichii ET-3 culture medium (total daily intake of 3g solid content and 283.5?g of DHNA; active group) or placebo (unfermented product) during two 1-week supplementation periods separated by a 4-week washout period. In Study 2, 11 healthy men took four tablets of P. freudenreichii ET-3 culture medium per day (total daily intake of 0.267g solid content and 22.5?g of DHNA) for a period of 13weeks. In both studies, hematological, clinical chemistry, and urinary parameters were measured before and after each supplementation period and gastrointestinal symptoms were assessed by questionnaire. In Study 1, there were no statistically significant differences between placebo and active supplementation periods in any measured parameter and the incidence of gastrointestinal symptoms were similar between groups. In Study 2, total protein, white blood cell count, hemoglobin, and mean corpuscular hemoglobin concentration decreased significantly from baseline and mean corpuscular volume and urine pH increased from baseline. The changes in hematological parameters were deemed not to be due to P. freudenreichii ET-3 culture medium supplementation given that all parameters remained within normal ranges and were not consistent with any clinically meaningful effect. PMID:21172397

  7. Equine influenza culture methods.

    PubMed

    Chambers, Thomas M; Reedy, Stephanie E

    2014-01-01

    Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not. PMID:24899449

  8. 7 CFR 1.417 - Review period.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... days after the comment period, pursuant to 36 CFR 233.190(h)(2). The request must be postmarked no... Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620 et seq.) § 1.417 Review period. (a) Review...

  9. 7 CFR 1.417 - Review period.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... days after the comment period, pursuant to 36 CFR 233.190(h)(2). The request must be postmarked no... Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620 et seq.) § 1.417 Review period. (a) Review...

  10. Rat retina shows robust circadian expression of clock and clock output genes in explant culture

    PubMed Central

    Buonfiglio, Daniella C.; Malan, André; Sandu, Cristina; Jaeger, Catherine; Cipolla-Neto, José; Hicks, David

    2014-01-01

    Purpose Circadian rhythms are central to vision and retinal physiology. A circadian clock located within the retina controls various rhythmic processes including melatonin synthesis in photoreceptors. In the present study, we evaluated the rhythmic expression of clock genes and clock output genes in retinal explants maintained for several days in darkness. Methods Retinas were dissected from Wistar rats, either wild-type or from the Per1-luciferase transgenic line housed under a daily 12 h:12 h light-dark cycle (LD12/12), and put in culture at zeitgeber time (ZT) 12 on semipermeable membranes. Explants from wild-type rats were collected every 4 h over 3 days, and total RNA was extracted, quantified, and reverse transcribed. Gene expression was assessed with quantitative PCR, and the periodicity of the relative mRNA amounts was assessed with nonlinear least squares fitting to sine wave functions. Bioluminescence in explants from Per1-luciferase rats was monitored for several days under three different culture protocols. Results Rhythmic expression was found for all studied clock genes and for clock downstream targets such as c-fos and arylalkylamine N-acetyltransferase (Aanat) genes. Clock and output genes cycled with relatively similar periods and acrophases (peaks of expression during subjective night, except c-fos, which peaked around the end of the subjective day). Data for Per1 were confirmed with bioluminescence monitoring, which also permitted culture conditions to be optimized to study the retina clock. Conclusions Our work shows the free-running expression profile of multiple clock genes and potential clock targets in mammalian retinal explants. This research further strengthens the notion that the retina contains a self-sustained oscillator that can be functionally characterized in organotypic culture. PMID:24940028

  11. Effect of Serum from Chickens Treated with Clenbuterol on Myosin Accumulation, Beta-Adrenergic Receptor Population, and Cyclic AM Synthesis in Embryonic Chicken Skeletal Muscle Cell Cultures

    NASA Technical Reports Server (NTRS)

    Young, R. B.; Bridge, K. Y.; Wuethrich, A. J.; Hancock, D. L.; Whitaker, Ann F. (Technical Monitor)

    2001-01-01

    Broiler chickens at 35 days of age were fed 1 ppm clenbuterol for 14 days. This level of dietary clenbuterol led to 5-7% increases in weights of leg and breast muscle tissue. At the end of the 14-day period, serum was prepared from both control and clenbuterol-treated chickens and was then employed as a component of cell culture media at a final concentration of 20% (v/v). Muscle cell cultures were prepared from both the leg and breast muscle groups of twelve-day chick embryos. Treatment groups included control chicken serum to which 10 nM, 50 nM, and 1 micron clenbuterol had been added, as well as cells grown in media containing 10% horse serum. Cultures were subjected to each treatment for 3 days beginning on the seventh day in culture. Neither the percent fusion nor the number of nuclei in myotubes were significantly affected by any of the treatments. The quantity of MHC was not increased by serum from clenbuterol-treated chickens in either breast and leg muscle cultures; however, MHC quantity was 50- 100% higher in cultures grown in control chicken serum to which 10 nM and 50 nM clenbuterol had also been added. The Beta-AR population was 4,000-7,000 Beta-AR per cell in cultures grown in chicken serum, with leg muscle cultures having approximately 25-30% more receptors than breast muscle cultures. Receptor population was not significantly affected by the presence of clenbuterol or by the presence of serum from clenbuterol-treated chickens. In contrast, the Beta-AR population in leg and breast muscle cultures grown in the presence of 10% horse serum was 18,000-20,000 Beta-AR per cell. Basal concentration of cAMP was not significantly affected by any of the treatments. When cultures grown in chicken serum were stimulated for 10 min with 1 micron isoproterenol, limited increases of 12-20% in cAMP concentration above basal levels were observed. However, when cultures grown in the presence of horse serum were stimulated with 1 micron isoproterenol, increases of 600-800 % in cAMP concentration above basal levels were observed. Thus, not only did cells grown in horse serum have a higher Beta-AR population, each receptor had a higher capacity for cAMP synthesis following isoproterenol stimulation. Finally, the hypothesis was tested that clenbuterol exerts its action on muscle protein content by changes in cAMP concentration. No correlation was apparent between basal cAMP concentration and MHC content.

  12. Cultural Themes.

    ERIC Educational Resources Information Center

    Roy, Loriene, Comp.

    Part of a larger report on the Four Directions Project, an American Indian technology innovation project, this section includes 10 "pathfinders" to locating information on Native American cultural themes. The pathfinders were designed by students in the Graduate School of Library and Information Science at the University of Texas at Austin in…

  13. Shanzhai Culture

    E-print Network

    Hacker, Randi

    2011-09-07

    . Which it isn't. The shanzhai culture is deeply entrenched here. Shanzhai, fake, counterfeit, bogus. Shanzhai copies of most Western brand name items are available. Ripoff Rolexes? Check. Knockoff Nikes? Check. Bogus Blahniks? Check. And it doesn't stop...

  14. Hydroponic Culture

    ERIC Educational Resources Information Center

    Steucek, G. L.; Yurkiewicz, W. J.

    1973-01-01

    Describes a hydroponic culture technique suitable for student exercises in biology. This technique of growing plants in nutrient solutions enhances plant growth, and is an excellent way to obtain intact plants with root systems free of soil or other particulate matter. (JR)

  15. Urine Culture

    MedlinePLUS

    ... get UTIs more often than males. Even school-age females may have frequent UTIs. For males with a culture-proven UTI, the doctor may order further tests to rule out the presence of a kidney stone or structural abnormality that could cause the infection. ...

  16. CULTURE SHOCK.

    ERIC Educational Resources Information Center

    WEINSTEIN, GERALD; AND OTHERS

    IN A PANEL, GEORGE BRAGLE AND NATHAN GOULD STRESS TEACHER PREPARATION TO COPE WITH THE THREATENING IMPACT OF CULTURE OR REALITY SHOCK. THEY RECOMMEND MODIFYING THE ATTITUDES OF TEACHERS BY ALTERING THEIR PERCEPTIONS, PROVIDING THEM WITH DIRECT EXPERIENCE WITH THE SOCIOCULTURAL MILIEU OF GHETTO SCHOOLS, AND REQUIRING THEM TO TAKE COURSES IN THE…

  17. Dual periodicities in energetic electrons at Saturn

    NASA Astrophysics Data System (ADS)

    Carbary, J. F.; Mitchell, D. G.; Krimigis, S. M.; Krupp, N.

    2009-10-01

    The fluxes of energetic electrons (110-365 keV) in Saturn's magnetosphere were subjected to Lomb periodogram analyses for 1677 days from late 2004 to the middle of 2009. The electrons generally exhibited strong periodicities at 10.82 hours and 10.60 hours for much of the ˜4.6 year interval. The longer period was generally present all the time, while the shorter period became prominent during the last ˜600 days of the interval. The 10.8-hour signal appeared strongly in observations made north of the equator and weakly in the south, while the 10.6-hour signal appeared only for northern observations. The dual periodicity in the electrons is similar to that recently discovered in the Saturn kilometric radiation. Periods in the 10.6 to 10.8 hour range are similar to the sun-referenced periods of neutral winds measured in Saturn's polar atmospheres.

  18. 78 FR 53790 - Public Forum-Safety Culture: Enhancing Transportation Safety

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-30

    ... BOARD Public Forum--Safety Culture: Enhancing Transportation Safety On Tuesday and Wednesday, September... Culture: Enhancing Transportation Safety.'' The forum will begin at 9:00 a.m. on both days and is open to... Culture; Organizational Leadership Perspectives on Safety Culture; Safety Culture Management and...

  19. 75 FR 22725 - Express Mail Next Day Delivery Postage Refund Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... Next Day Delivery postage refunds when shipments are mailed each year during the time period of... facilities. Express Mail Second Day Delivery During the time period of December 22 through December 25... Express Mail Next Day Delivery Postage Refund Amendment AGENCY: Postal Service TM . ACTION: Proposed...

  20. 5 CFR 9901.407 - Minimum period of performance.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...Minimum period of performance. 9901.407...DEFENSE NATIONAL SECURITY PERSONNEL SYSTEM (NSPS) Performance Management § 9901...minimum period of performance is 90 calendar days...e.g., due to job change to a...

  1. 7 CFR 1230.639 - Additional absentee voter challenge period.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Additional absentee voter challenge period. (a) Absentee Voter Request List. The Absentee Voter Request List (Form LS-74) will be available for inspection during an additional challenge period of five business days (October 23,...

  2. 7 CFR 1230.639 - Additional absentee voter challenge period.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Additional absentee voter challenge period. (a) Absentee Voter Request List. The Absentee Voter Request List (Form LS-74) will be available for inspection during an additional challenge period of five business days (October 23,...

  3. 7 CFR 1230.639 - Additional absentee voter challenge period.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Additional absentee voter challenge period. (a) Absentee Voter Request List. The Absentee Voter Request List (Form LS-74) will be available for inspection during an additional challenge period of five business days (October 23,...

  4. 7 CFR 1230.639 - Additional absentee voter challenge period.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Additional absentee voter challenge period. (a) Absentee Voter Request List. The Absentee Voter Request List (Form LS-74) will be available for inspection during an additional challenge period of five business days (October 23,...

  5. 7 CFR 1230.639 - Additional absentee voter challenge period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Additional absentee voter challenge period. (a) Absentee Voter Request List. The Absentee Voter Request List (Form LS-74) will be available for inspection during an additional challenge period of five business days (October 23,...

  6. 2010 Stennis Day of Service

    NASA Technical Reports Server (NTRS)

    2010-01-01

    Rich Delgado, commanding officer of the Fleet Survey Team located at NASA's John C. Stennis Space Center, visits with Kertrina Watson Lewis, executive director of the HandsOn volunteer organization in New Orleans, during Day of Service activities Jan. 12. The Day of Service was part of the annual Martin Luther King Jr. Day observance at Stennis. During the day, Mississippi and Louisiana organizations visited the center to encourage employees to register and serve as volunteers for various community activities.

  7. 2010 Stennis Day of Service

    NASA Technical Reports Server (NTRS)

    2010-01-01

    Employees at NASA's John C. Stennis Space Center visit exhibits of volunteer organizations during their observance of Martin Luther King Jr. Day with a Day of Service on Jan. 12. During the day, Mississippi and Louisiana organizations visited the center to encourage employees to register and serve as volunteers for various community activities. The day's focus was emphasized again and again - great things can happen when individuals work together toward a common goal.

  8. 70 Days of Jupiter Winds

    NASA Technical Reports Server (NTRS)

    2001-01-01

    This global movie of 70 days of Jupiter's cloud movements photographed by NASA's Cassini spacecraft shows that zones of eastward and westward winds cover the planet virtually from pole to pole.

    Cassini's narrow-angle camera captured the images of Jupiter's atmosphere from October 1 to December 9, 2000, in the near-infrared region of the spectrum. The view here is a cylindrical projection centered in the planet's equator.

    The movie allows tracking of individual storms' movements in the familiar zonal bands of the lower latitudes, in the swirling turbulence around the Great Red Spot and in the high latitudes where still images show chaotic mottling instead of stripes.

    Cassini collected images of Jupiter for months before and after it passed the planet on December 30, 2000. Six or more images of the planet in each of several spectral filters were taken at evenly spaced intervals over the course of Jupiter's 10-hour rotation period. The entire sequence was repeated generally every other Jupiter rotation, yielding views of every sector of the planet at least once every 20 hours.

    The images used for the movie shown here were taken every 20 hours through a filter centered at a wavelength of 756 nanometers, where there are almost no absorptions in the planet's atmosphere. Six images covering each rotation were mosaiced together to form a cylindrical map extending from 75 degrees north to 75 degrees south in latitude and covering 360 degrees in longitude. The movie consists of 84 such maps, spanning 70Earth days in time or 168 Jupiter rotations.

    For more information, see the Cassini Project home page, http://www.jpl.nasa.gov/cassini/ and the Cassini Imaging Team home page, http://ciclops.lpl.arizona.edu/ciclops/ .

    Cassini is a cooperative project of NASA, the European Space Agency and the Italian Space Agency. The Jet Propulsion Laboratory, a division of the California Institute of Technology in Pasadena, manages the Cassini mission for NASA's Office of Space Science, Washington, D.C.

  9. Family Day Care Provider Handbook

    ERIC Educational Resources Information Center

    New York State Office of Children and Family Services, 2006

    2006-01-01

    Family day care providers are responsible for creating a high-quality program where children have opportunities to grow, learn and thrive. Part of providing high-quality child care includes complying with the family day care regulations from the New York State Office of Children and Family Services (OCFS). This Handbook will help day care…

  10. Perspectives on Infant Day Care.

    ERIC Educational Resources Information Center

    Elardo, Richard, E.; Pagan, Betty, Ed.

    These proceedings of the first annual SACUS workshop on infant day care contain the papers presented at the conference, plus an appendix--Developmental Objectives for Infants and Toddlers. The papers are: "Infant Day Care--Fads, Facts, and Fancies" by Bettye M. Caldwell; "Family Day Care""A Broad Perspective" by Malcolm S. Host; "Getting…

  11. The 4 Day School Week

    ERIC Educational Resources Information Center

    Dam, Ai

    2006-01-01

    Colorado law requires school districts to schedule 1080 hours per year of instructional time for secondary schools and 990 instructional hours for elementary schools. The 1080 hours equate to six hours per day for 180 days. The 990 hours equate to five and one-half hours per day. Up to 24 hours may be counted for parent-teacher conferences, staff…

  12. STS-90 Day 16 Highlights

    NASA Technical Reports Server (NTRS)

    1998-01-01

    On this sixteenth day of the STS-90 mission, the flight crew, Cmdr. Richard A. Searfoss, Pilot Scott D. Altman, and Mission Specialists Richard M. Linnehan, Dafydd Rhys Williams and Kathryn P. Hire, and Payload Specialists Jay C. Buckey and James A. Pawelczyk begin a busy day preparing for their return to the Kennedy Space Center later in the day.

  13. Guides for Day Care Licensing.

    ERIC Educational Resources Information Center

    Child Development Services Bureau (DHEW/OCD), Washington, DC.

    This booklet provides source materials for the development of state and local regulations applicable to day care service facilities. Sections discuss: (1) the Model State Day Care Licensing Act, (2) Day care program and staffing, (3) Health and sanitation, (4) Fire and safety regulations, (5) Principles of zoning, and (6) Principles of…

  14. Uncoupling of attenuated myo-(3H)inositol uptake and dysfunction in Na(+)-K(+)-ATPase pumping activity in hypergalactosemic cultured bovine lens epithelial cells

    SciTech Connect

    Cammarata, P.R.; Tse, D.; Yorio, T. )

    1991-06-01

    Attenuation of both the active transport of myo-inositol and Na(+)-K(+)-ATPase pumping activity has been implicated in the onset of sugar cataract and other diabetic complications in cell culture and animal models of the disease. Cultured bovine lens epithelial cells (BLECs) maintained in galactose-free Eagle's minimal essential medium (MEM) or 40 mM galactose with and without sorbinil for up to 5 days were examined to determine the temporal effects of hypergalactosemia on Na(+)-K(+)-ATPase and myo-inositol uptake. The Na(+)-K(+)-ATPase pumping activity after 5 days of continuous exposure to galactose did not change, as demonstrated by 86Rb uptake. The uptake of myo-(3H)inositol was lowered after 20 h of incubation in galactose and remained below that of the control throughout the 5-day exposure period. The coadministration of sorbinil to the galactose medium normalized the myo-(3H)inositol uptake. No significant difference in the rates of passive efflux of myo-(3H)inositol or 86Rb from preloaded galactose-treated and control cultures was observed. Culture-media reversal studies were also carried out to determine whether the galactose-induced dysfunction in myo-inositol uptake could be corrected. BLECs were incubated in galactose for 5 days, then changed to galactose-free physiological medium with and without sorbinil for a 1-day recovery period. myo-Inositol uptake was reduced to 34% of control after 6 days of continuous exposure to galactose. Within 24 h of media reversal, myo-inositol uptake returned to or exceeded control values in BLECs switched to either MEM or MEM with sorbinil.2+ reversible and occurred independently of changes in Na(+)-K(+)-ATPase pumping activity in cultured lens epithelium, indicating that the two parameters are not strictly associated and that the deficit in myo-inositol uptake occurs rapidly during hypergalactosemia.

  15. On the signature of the quasi-3-day wave in the thermosphere during the January 2010 URSI World Day Campaign

    E-print Network

    California at Berkeley, University of

    with incoherent scatter radars are available. While there is no evidence that the occurrence of this 3-day wave during this period, including the first-ever detection of a 3-day wave with an incoherent scatter radar 105 km altitude. Evidence of an interaction between this wave and the diurnal tide is seen between 82

  16. Evolution of periodicity in periodical cicadas

    PubMed Central

    Ito, Hiromu; Kakishima, Satoshi; Uehara, Takashi; Morita, Satoru; Koyama, Takuya; Sota, Teiji; Cooley, John R.; Yoshimura, Jin

    2015-01-01

    Periodical cicadas (Magicicada spp.) in the USA are famous for their unique prime-numbered life cycles of 13 and 17 years and their nearly perfectly synchronized mass emergences. Because almost all known species of cicada are non-periodical, periodicity is assumed to be a derived state. A leading hypothesis for the evolution of periodicity in Magicicada implicates the decline in average temperature during glacial periods. During the evolution of periodicity, the determinant of maturation in ancestral cicadas is hypothesized to have switched from size dependence to time (period) dependence. The selection for the prime-numbered cycles should have taken place only after the fixation of periodicity. Here, we build an individual-based model of cicadas under conditions of climatic cooling to explore the fixation of periodicity. In our model, under cold environments, extremely long juvenile stages lead to extremely low adult densities, limiting mating opportunities and favouring the evolution of synchronized emergence. Our results indicate that these changes, which were triggered by glacial cooling, could have led to the fixation of periodicity in the non-periodical ancestors. PMID:26365061

  17. Evolution of periodicity in periodical cicadas.

    PubMed

    Ito, Hiromu; Kakishima, Satoshi; Uehara, Takashi; Morita, Satoru; Koyama, Takuya; Sota, Teiji; Cooley, John R; Yoshimura, Jin

    2015-01-01

    Periodical cicadas (Magicicada spp.) in the USA are famous for their unique prime-numbered life cycles of 13 and 17 years and their nearly perfectly synchronized mass emergences. Because almost all known species of cicada are non-periodical, periodicity is assumed to be a derived state. A leading hypothesis for the evolution of periodicity in Magicicada implicates the decline in average temperature during glacial periods. During the evolution of periodicity, the determinant of maturation in ancestral cicadas is hypothesized to have switched from size dependence to time (period) dependence. The selection for the prime-numbered cycles should have taken place only after the fixation of periodicity. Here, we build an individual-based model of cicadas under conditions of climatic cooling to explore the fixation of periodicity. In our model, under cold environments, extremely long juvenile stages lead to extremely low adult densities, limiting mating opportunities and favouring the evolution of synchronized emergence. Our results indicate that these changes, which were triggered by glacial cooling, could have led to the fixation of periodicity in the non-periodical ancestors. PMID:26365061

  18. Academic Culture and Campus Culture of Universities

    ERIC Educational Resources Information Center

    Shen, Xi; Tian, Xianghong

    2012-01-01

    Academic culture of universities mainly consists of academic outlooks, academic spirits, academic ethics and academic environments. Campus culture in a university is characterized by individuality, academic feature, opening, leading, variety and creativity. The academic culture enhances the construction of campus culture. The campus culture

  19. HepaRG culture in tethered spheroids as an in vitro three-dimensional model for drug safety screening.

    PubMed

    Wang, Zenan; Luo, Xiaobei; Anene-Nzelu, Chukwuemeka; Yu, Yu; Hong, Xin; Singh, Nisha Hari; Xia, Lei; Liu, Side; Yu, Hanry

    2015-08-01

    Conventional two-dimensional (2D) monolayer cultures of HepaRG cells allow in vitro maintenance of many liver-specific functions. However, cellular dedifferentiation and functional deterioration over an extended culture period in the conventional 2D HepaRG culture have hampered its applications in drug testing. To address this issue, we developed tethered spheroids of HepaRG cells on Arg-Gly-Asp (RGD) and galactose-conjugated substratum with an optimized hybrid ratio as an in vitro three-dimensional (3D) human hepatocyte model. The liver-specific gene expression level and drug metabolizing enzyme activities in HepaRG-tethered spheorids were markedly higher than those in 2D cultures throughout the culture period of 7?days. The inducibility of three major cytochrome P450 (CYP) enzymes, namely CYP1A2, CYP2B6 and CYP3A4, was improved in both mRNA and activity level in tethered spheroids. Drug-induced cytotoxic responses to model hepatotoxins (acetaminophen, chlorpromazine and ketoconazole) in tethered spheroids were comparable to 2D cultures as well as other studies in the literature. Our results suggested that the HepaRG-tethered spheroid would be an alternative in vitro model suitable for drug safety screening. PMID:25512232

  20. Culturing Conceptions: From First Principles

    ERIC Educational Resources Information Center

    Roth, Wolff-Michael; Lee, Yew Jin; Hwang, SungWon

    2008-01-01

    Over the past three decades, science educators have accumulated a vast amount of information on conceptions--variously defined as beliefs, ontologies, cognitive structures, mental models, or frameworks--that generally (at least initially) have been derived from interviews about certain topics. During the same time period, cultural studies has…

  1. Cultural Enrichment through Community Action.

    ERIC Educational Resources Information Center

    Wilson, O. J.

    This project was conceived as a technique for helping to eliminate a cultural void in the areas of art, music, and theatre in the service area of Western Kentucky University. To implement this concept, demonstrations were conducted in art, music, theatre, and in library and lecture resources in 16 counties over a four-year period. The attendance…

  2. 7 CFR 3015.22 - Starting date of retention period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Starting date of retention period. 3015.22 Section... Access Requirements § 3015.22 Starting date of retention period. (a) General. The retention period starts... funding period starts on the day the recipient submits to USDA its annual or final expenditure report...

  3. Culture of human septal chondrocytes in a rotary bioreactor

    PubMed Central

    Reuther, Marsha S.; Briggs, Kristen K.; Wong, Van W.; Chang, Angela A.; Schumacher, Barbara L.; Masuda, Koichi; Sah, Robert L.; Watson, Deborah

    2014-01-01

    Objective 1) To show that extracellular matrix deposition is possible in 3-dimensional culture of human septal chondrocytes cultured in a rotary bioreactor as well as in static conditions. 2) To demonstrate that the biomechanical properties of human septal chondrocytes cultured in a bioreactor and static culture amplify with time. Study Design Prospective, basic science Setting Research laboratory Methods Human septal chondrocytes from nine donors were expanded in monolayer and seeded in alginate beads. The beads were cultured in a rotary bioreactor for 21 days in media supplemented with growth factors and human serum, using static culture as the control. Biochemical and biomechanical properties of the beads were measured. Results Glycosaminoglycan (GAG) accumulation significantly increased during two measured time intervals, zero to 10 days and 10 to 21 days (p<0.01). No significant difference was seen between the static and bioreactor conditions. However, substantial type II collagen production was demonstrated in the beads terminated at day 21 of culture in both conditions. In addition, the biomechanical properties of the beads were enhanced at 21 days in comparison to beads from days 0 and 10. Conclusion Human septal chondrocytes cultured in alginate beads exhibit significant matrix deposition and improved biomechanical properties after 21 days. Enhanced matrix deposition during bead culture will expectantly lead to formation of neocartilage that is comparable to native tissue. Matrix production in beads is supported by the use of a rotary bioreactor. PMID:22597575

  4. The American Child: A Cultural Studies Reader.

    ERIC Educational Resources Information Center

    Levander, Caroline F., Ed.; Singley, Carol J., Ed.

    There has been increased critical interest in the child as a rich and varied site of cultural inscription. Scholars from a wide range of disciplines have turned their attention to the child in order to interrogate how it comes to represent, and often codify, the prevailing ideologies of a given culture or historical period. Noting that narratives…

  5. Children on the Move: Third Culture Kids.

    ERIC Educational Resources Information Center

    Gillies, Warna D.

    1998-01-01

    Defines Third Culture Kids as the children of diplomats, missionaries, business persons, and military personnel who live outside their native countries for periods of time. Considers the problems and advantages of travel and uncertain cultural identity for these children. Suggests instructional strategies for working with internationally mobile…

  6. Day 1 Day 2 Day 3 Migra&on Distance (m)

    E-print Network

    Chiao, Jung-Chih

    . The devices were mounted on a standard polystyrene tissue culture plate. MDA-MB-231, a breast cancer cell line.0062, respectively) was found between the MDA-MB-231 cells exposed to EGF and the ones not exposed to EGF. Figure 1 displays the average migration distance of MDA-MB-231 for both conditions. MDA-MB-231 in confluent culture

  7. 8-14 Day Outlook 30-Day and 90-Day Seasonal Outlook

    E-print Network

    8-14 Day Outlook 30-Day and 90-Day Seasonal Outlook ENSO Advisory Climate Diagnostics and Prediction Workshop Monthly Climate Bulletin Drought Advisory Drought Outlook Ozone Winter Summary UV Index 4. First experimental drought outlook map, 1999 35 5. African countries that participated in CPC

  8. Giving Thanks: Observing Thanksgiving, Kwanzaa, and Day of the Dead.

    ERIC Educational Resources Information Center

    Dever, Martha T.; Barta, James J.

    1997-01-01

    Describes a primary-grade curriculum unit organized around the theme of "giving thanks" and encompassing the holidays of Thanksgiving, Kwanzaa, and Day of the Dead. Provides historical background and cultural context for each holiday, engagement activities, investigation activities, sharing activities, and a short list of related children's…

  9. In the Schools: California Treat: Three Days in Five Ecosystems.

    ERIC Educational Resources Information Center

    Rigby, Jennifer A.

    1986-01-01

    Describes a 3-day program sponsored by the Orange County Marine Institute that provides biological, cultural, and historical learning experiences. Discusses the setting and activities of the five ecosystems explored by the students. The Chaparral to Ocean Science Camp includes chaparral, riparian, woodland, intertidal, and pelagic environments.…

  10. Mechanical properties evolution of a PLGA-PLCL composite scaffold for ligament tissue engineering under static and cyclic traction-torsion in vitro culture conditions.

    PubMed

    Kahn, Cyril J F; Ziani, Kahina; Zhang, Ye Min; Liu, Jian; Tran, Nguyen; Babin, Jérôme; de Isla, Natalia; Six, Jean-Luc; Wang, Xiong

    2013-01-01

    This study aims to investigate the in vitro degradation of a poly(L-lactic-co-glycolic acid)-poly(L-lactic-co-?-caprolactone) (PLGA-PLCL) composite scaffold's mechanical properties under static culture condition and 2?h period per day of traction-torsion cyclic culture conditions of simultaneous 10% uniaxial strain and 90° of torsion cycles at 0.33?Hz. Scaffolds were cultured in static conditions, during 28?days, with or without cell seeded or under dynamic conditions during 14?days in a bioreactor. Scaffolds' biocompatibility and proliferation were investigated with Alamar Blue tests and cell nuclei staining. Scaffolds' mechanical properties were tested during degradation by uniaxial traction test. The PLGA-PLCL composite scaffold showed a good cytocompatibility and a high degree of colonization in static conditions. Mechanical tests showed a competition between two process of degradation which have been associated to hydrolytic and enzymatic degradation for the reinforce yarn in poly(L-lactic-co-glycolic acid) (PLGA). The enzymatic degradation led to a decrease effect on mechanical properties of cell-seeded scaffolds during the 21st days, but the hydrolytic degradation was preponderant at day 28. In conclusion, the structure of this scaffold is adapted to culture in terms of biocompatibility and cell orientation (microfiber) but must be improved by delaying the degradation of it reinforce structure in PLGA. PMID:23647247

  11. 75 FR 41793 - Child and Adult Care Food Program: National Average Payment Rates, Day Care Home Food Service...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ...National Average Payment Rates, Day Care Home Food Service Payment Rates...Rates for Sponsoring Organizations of Day Care Homes for the Period July 1, 2010...afterschool care centers, and adult day care centers; the food service...

  12. 78 FR 45176 - Child and Adult Care Food Program: National Average Payment Rates, Day Care Home Food Service...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ...National Average Payment Rates, Day Care Home Food Service Payment Rates...Rates for Sponsoring Organizations of Day Care Homes for the Period July 1, 2013...afterschool care centers, and adult day care centers; the food service...

  13. 76 FR 44573 - Child and Adult Care Food Program: National Average Payment Rates, Day Care Home Food Service...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-26

    ...National Average Payment Rates, Day Care Home Food Service Payment Rates...Rates for Sponsoring Organizations of Day Care Homes for the Period July 1, 2011...Rates for Sponsoring Organizations of Day Care Homes per Home (Per month rates...

  14. 76 FR 43254 - Child and Adult Care Food Program: National Average Payment Rates, Day Care Home Food Service...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ...National Average Payment Rates, Day Care Home Food Service Payment Rates...Rates for Sponsoring Organizations of Day Care Homes for the Period July 1, 2011...afterschool care centers, and adult day care centers; the food service...

  15. Cultural neurolinguistics

    PubMed Central

    Chen, Chuansheng; Xue, Gui; Mei, Leilei; Chen, Chunhui; Dong, Qi

    2010-01-01

    As the only species that evolved to possess a language faculty, humans have been surprisingly generative in creating a diverse array of language systems. These systems vary in phonology, morphology, syntax, and written forms. Before the advent of modern brain-imaging techniques, little was known about how differences across languages are reflected in the brain. This chapter aims to provide an overview of an emerging area of research — cultural neurolinguistics — that examines systematic cross-cultural/crosslinguistic variations in the neural networks of languages. We first briefly describe general brain networks for written and spoken languages. We then discuss language-specific brain regions by highlighting differences in neural bases of different scripts (logographic vs. alphabetic scripts), orthographies (transparent vs. nontransparent orthographies), and tonality (tonal vs. atonal languages). We also discuss neural basis of second language and the role of native language experience in second-language acquisition. In the last section, we outline a general model that integrates culture and neural bases of language and discuss future directions of research in this area. PMID:19874968

  16. A simplified protocol for the isolation and culture of cardiomyocytes and progenitor cells from neonatal mouse ventricles.

    PubMed

    Vidyasekar, Prasanna; Shyamsunder, Pavithra; Santhakumar, Rajalakshmi; Arun, Rajpranap; Verma, Rama Shanker

    2015-10-01

    The neonatal heart is a very useful tool for the study of biochemical pathways and properties of cardiomyocytes and as it has the potential to regenerate for a brief period of time from birth; it is also useful to study cardiac regeneration. However, as the heart matures, this proficiency for regeneration is reduced. This regenerative potential may be influenced by the microenvironment of the heart in the early stages of postnatal development and therefore, cell cultures derived at this stage may contain functional cardiomyocytes and progenitor cells. The aim of this study was to identify key steps in the isolation and culture of such early stage-neonatal mouse hearts to allow maximum migration of cardiomyocytes from the explant and their maintenance as functional, long term cultures. Explant cultures of mouse ventricles preserved 3-dimensional structure and generated migrating layers of cardiomyocytes that expressed alpha sarcomeric actin which could be further sub-cultured by enzymatic dissociation. Western blotting demonstrated expression of c-KIT, GATA4, alpha sarcomeric actin and connexin43 proteins after 20 days of explant culture. ACTA1, GATA4, and CX43 continued to express in five weeks old explant cultures while the c-KIT protein was expressed up to two passages during sub-culture. Real time PCR and SQRT PCR also demonstrated gene expression of cardiomyocyte markers in long term cultures. Migrating cells from the explants assembled into contracting spheroids after subculture and expressed the c-KIT protein. Progenitor markers CD44, CD90, and extracellular proteins, periostin and vimentin demonstrated the preservation of cellular heterogeneity in such cultures. Supplementation with Hydrocortisone maintained a cardioprotective environment and reduced the non-myocyte population. This is an optimized and efficient method for the generation of neonatal heart cultures that is not labor intensive and does not require supplementation with cytokines. PMID:26153430

  17. Familial Periodic Paralyses

    MedlinePLUS

    ... two most common types of periodic paralyses are: Hypokalemic periodic paralysis is characterized by a fall in potassium levels ... develop chronic muscle weakness later in life. Hyperkalemic periodic ... than the hypokalemic form. Muscle spasms are common. Is there any ...

  18. Pepsinogen synthesis during long-term culture of porcine chief cells.

    PubMed

    Heim, H K; Piller, M; Schwede, J; Kilian, P; Netz-Piepenbrink, S; Sewing, K F

    1997-10-30

    The purpose of this study was to characterize time-dependent changes in pepsinogen (PG) synthesis of porcine gastric chief cells during long-term monolayer culture. Porcine chief cells were isolated by pronase/collagenase treatment of fundic mucosa and enriched by density gradient and counterflow centrifugation. PG isoenzymes were identified in [L-35S]methionine-labelled cultured chief cells by native polyacrylamide gel electrophoresis followed by phosphor imager analysis, protease detection and immunoblots with specific PG A and C antibodies. The obtained results suggest that porcine chief cell cultures, after an initial settling period, reached an approximate steady state in total protein content and synthesis as well as in PG content and isoenzyme pattern from days 3 to 9 of culture. The latter was characterized by the presence of at least two PG A and two PG C isoenzymes. During the supposed steady-state total PG synthesis averaged out at 34 +/- 2% of total protein synthesis, as detected by [L-35S]methionine incorporation, due to the synthesis of, mainly, PG A2 and, to a much lesser extent, PG C and A1. In line with an active secretion, PG A2 proportion was on average significantly higher in released (44 +/- 3%) than in intracellular labelled proteins (19 +/- 2%). In addition, PG release from chief cells cultured for 6 and 9 days could be stimulated by cholecystokinin-octapeptide. These data suggest that porcine chief cells in monolayer culture are a model well suited for the quantitative and qualitative characterization of PG isoenzyme synthesis and release during long-term investigations, for which an establishment of a culture steady state appears to be a useful prerequisite. PMID:9398083

  19. PSY 344. Psychology and Culture SDSU Fall 2015 Schedule # 22848

    E-print Network

    Gallo, Linda C.

    PSY 344. Psychology and Culture SDSU ­ Fall 2015 ­ Schedule # 22848 Class Meetings Class Day: Tuesday Class Times: 2:00 ­ 3:15 PM Class Location: West Commons (WC) 230 Instructor: Dr. Thierry Devos of the course is to introduce you to the field of cultural psychology. This course will explore how culture

  20. Mesenchymal stem cells cultured under hypoxia escape from senescence via down-regulation of p16 and extracellular signal regulated kinase.

    PubMed

    Jin, Yonghui; Kato, Tomohisa; Furu, Moritoshi; Nasu, Akira; Kajita, Yoichiro; Mitsui, Hiroto; Ueda, Michiko; Aoyama, Tomoki; Nakayama, Tomitaka; Nakamura, Takashi; Toguchida, Junya

    2010-01-15

    Hypoxia has been considered to affect the properties of tissue stem cells including mesenchymal stem cells (MSCs). Effects of long periods of exposure to hypoxia on human MSCs, however, have not been clearly demonstrated. MSCs cultured under normoxic conditions (20% pO(2)) ceased to proliferate after 15-25 population doublings, while MSCs cultured under hypoxic conditions (1% pO(2)) retained the ability to proliferate with an additional 8-20 population doublings. Most of the MSCs cultured under normoxic conditions were in a senescent state after 100days, while few senescent cells were found in the hypoxic culture, which was associated with a down-regulation of p16 gene expression. MSCs cultured for 100days under hypoxic conditions were superior to those cultured under normoxic conditions in the ability to differentiate into the chondro- and adipogenic, but not osteogenic, lineage. Among the molecules related to mitogen-activated protein kinase (MAPK) signaling pathways, extracellular signal regulated kinase (ERK) was significantly down-regulated by hypoxia, which helped to inhibit the up-regulation of p16 gene expression. Therefore, the hypoxic culture retained MSCs in an undifferentiated and senescence-free state through the down-regulation of p16 and ERK. PMID:20034468

  1. Mesenchymal stem cells cultured under hypoxia escape from senescence via down-regulation of p16 and extracellular signal regulated kinase

    SciTech Connect

    Jin, Yonghui; Kato, Tomohisa; Furu, Moritoshi; Nasu, Akira; Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University ; Kajita, Yoichiro; Department of Urology, Graduate School of Medicine, Kyoto University ; Mitsui, Hiroto; Department of Musculoskeletal Medicine, Graduate School of Medical Sciences, Nagoya City University ; Ueda, Michiko; Aoyama, Tomoki; Nakayama, Tomitaka; Nakamura, Takashi; Toguchida, Junya; Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University; Center for iPS Cell Research and Application, Institute for Integrated Cell - Material Sciences, Kyoto University

    2010-01-15

    Hypoxia has been considered to affect the properties of tissue stem cells including mesenchymal stem cells (MSCs). Effects of long periods of exposure to hypoxia on human MSCs, however, have not been clearly demonstrated. MSCs cultured under normoxic conditions (20% pO{sub 2}) ceased to proliferate after 15-25 population doublings, while MSCs cultured under hypoxic conditions (1% pO{sub 2}) retained the ability to proliferate with an additional 8-20 population doublings. Most of the MSCs cultured under normoxic conditions were in a senescent state after 100 days, while few senescent cells were found in the hypoxic culture, which was associated with a down-regulation of p16 gene expression. MSCs cultured for 100 days under hypoxic conditions were superior to those cultured under normoxic conditions in the ability to differentiate into the chondro- and adipogenic, but not osteogenic, lineage. Among the molecules related to mitogen-activated protein kinase (MAPK) signaling pathways, extracellular signal regulated kinase (ERK) was significantly down-regulated by hypoxia, which helped to inhibit the up-regulation of p16 gene expression. Therefore, the hypoxic culture retained MSCs in an undifferentiated and senescence-free state through the down-regulation of p16 and ERK.

  2. Microbial Diversity within Early-Stage Cultured Panulirus ornatus Phyllosomas?

    PubMed Central

    Payne, Matthew S.; Hall, Mike R.; Sly, Lindsay; Bourne, David G.

    2007-01-01

    A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiological techniques to characterize the bacterial community associated with cultured, developmental stage PI to PII P. ornatus phyllosomas. Scanning electron microscopy demonstrated colonization of phyllosomas by filamentous, rod-shaped, and coccus-shaped bacteria. A clone library constructed from dead phyllosomas sampled from the larval rearing tank on day 10 was dominated by Thiothrix-affiliated sequences (56% of clones). A comparable library from live phyllosomas also contained Thiothrix-affiliated sequences, though these only represented 19% of clones within the library. Fluorescent in situ hybridization (FISH) confirmed identification of the filamentous bacteria as Thiothrix sp., being present on dead phyllosomas. FISH also identified Leucothrix sp. and Vibrio sp., as well as a range of other rod- and coccus-shaped bacteria, colonizing both live and dead phyllosomas. The development of the microbial community associated with phyllosomas was monitored through a standard larval rearing run using denaturing gradient gel electrophoresis (DGGE). Vibrio sp.-affiliated bands dominated the profiles of live animals through the rearing period and dead phyllosomas sampled on selected days. The population of Vibrio sp. associated with phyllosomas was monitored with culture-based analysis on selective media and demonstrated to increase significantly on day 7, coinciding with the beginning of the larval molt. An isolated Vibrio harveyi strain demonstrated an identical 16S rRNA sequence with retrieved DGGE and clone library sequences. Colonization of phyllosomas with filamentous bacterial species potentially hinders the ability of the animals to molt and, combined with the added stress of the molt process, likely results in reduced immune function, allowing opportunistic pathogenic Vibrio sp. to cause larval mortalities. PMID:17220250

  3. "Every Day He Has a Dream to Tell": Classroom Literacy Curriculum in a Full-Day Kindergarten

    ERIC Educational Resources Information Center

    Heydon, Rachel; Moffatt, Lyndsay; Iannacci, Luigi

    2015-01-01

    Within an era of change to early childhood education and care, this case study of kindergarten classroom literacy curricula sought to understand the production and effects of the curriculum within one urban, Canadian full-day kindergarten that included culturally and linguistically diverse children. Central was a concern for the place of…

  4. PERIOD ERROR ESTIMATION FOR THE KEPLER ECLIPSING BINARY CATALOG

    SciTech Connect

    Mighell, Kenneth J.; Plavchan, Peter

    2013-06-15

    The Kepler Eclipsing Binary Catalog (KEBC) describes 2165 eclipsing binaries identified in the 115 deg{sup 2} Kepler Field based on observations from Kepler quarters Q0, Q1, and Q2. The periods in the KEBC are given in units of days out to six decimal places but no period errors are provided. We present the PEC (Period Error Calculator) algorithm, which can be used to estimate the period errors of strictly periodic variables observed by the Kepler Mission. The PEC algorithm is based on propagation of error theory and assumes that observation of every light curve peak/minimum in a long time-series observation can be unambiguously identified. The PEC algorithm can be efficiently programmed using just a few lines of C computer language code. The PEC algorithm was used to develop a simple model that provides period error estimates for eclipsing binaries in the KEBC with periods less than 62.5 days: log {sigma}{sub P} Almost-Equal-To - 5.8908 + 1.4425(1 + log P), where P is the period of an eclipsing binary in the KEBC in units of days. KEBC systems with periods {>=}62.5 days have KEBC period errors of {approx}0.0144 days. Periods and period errors of seven eclipsing binary systems in the KEBC were measured using the NASA Exoplanet Archive Periodogram Service and compared to period errors estimated using the PEC algorithm.

  5. Advances in cell culture

    SciTech Connect

    Maramorosch, K. )

    1987-01-01

    This book presents papers on advances in cell culture. Topics covered include: Genetic changes in the influenza viruses during growth in cultured cells; The biochemistry and genetics of mosquito cells in culture; and Tree tissue culture applications.

  6. Rethinking the Day of Silence

    ERIC Educational Resources Information Center

    Murphy, Adriana

    2013-01-01

    Back in 2006, 7th and 8th graders at Green Acres, the K-8 independent school where the author taught in suburban Maryland, participated in the Day of Silence. The Day of Silence is a national event: Students across the country take a one-day pledge of silence to show that they want to make schools safe for all students, regardless of their sexual…

  7. GRAND DANUBE PASSAGE: FEATURING SOFIA & PRAGUE DAY BY DAY ITINERARY

    E-print Network

    Toronto, University of

    this village date from Roman times. Tour the city centre, then drive through the scenic Iron Gate region of Pécs. Founded by the Romans, this pretty Hungarian city has an excellent collection of Roman ruins in to the Art Deco Imperial Hotel. The day is at leisure. Day 14 ~ Prague Excursion: Prague Castle and Old Town

  8. Zoning for Day Care (from Models for Day Care Licensing).

    ERIC Educational Resources Information Center

    Day Care and Child Development Council of America, Inc., Washington, DC.

    Recommendations and regulations regarding the zoning of child development day care programs are discussed. Zoning in general is discussed, as is the treatment of child development day care in zoning ordinance, the background of program planning, modular housing, the impelmentation of zoning, and model provisions regarding characteristics of…

  9. Study of the Half-Day/Full-Day Kindergarten Model

    ERIC Educational Resources Information Center

    McInroy, Thomas R.

    2012-01-01

    This case study and problem analysis was an in-depth investigation of the half-day/full-day kindergarten model by utilizing interviews and focus groups to provide insight from parents, teachers, and other district personnel as to how the model has impacted the social, emotional, and academic development of the participating students. This study…

  10. Extreme hail day climatology in Southwestern France

    NASA Astrophysics Data System (ADS)

    Berthet, C.; Wesolek, E.; Dessens, J.; Sanchez, J. L.

    2013-04-01

    The concentration of hail damage over a very few days of each warm season is a main characteristic of hail climatology in Southwestern France. The hailpad networks set up there in 1988 by the ANELFA hail prevention project have allowed the development of a new extreme hail day climatology which consists in examining the hail day data in the Atlantic region mainly under maritime conditions, and in the Pyrenean region, under continental and mountain influences. A pyramidal representation of each daily mean hailfall parameter (frequency per station, kinetic energy per hailfall, and kinetic energy per station), ranked by order of magnitude, allows a comparison of the mean and extreme values in the two regions, and reveals the strongest hail days in each one. During the 23-year period under study, 13 extreme situations (7 in the Atlantic region, 6 in the Pyrenean one) are highlighted, keeping the first three day ranking for each parameter. For each day, the synoptic situation, cell type, storm trajectories, and hail damages are examined, and numerical simulations with the WRF model, initialized by the CDAS/NCEP-NCAR reanalysis program, are considered. The study confirms that the Pyrenean region (20,000 km2) has experienced the major hail situations, with record values of each parameter for the two regions. On the frequency record day (16 April 2007), 17% of the hailpad stations were impacted, and on the kinetic energy record day (16 July 2009), the total hailstone energy reached 33 J/m2 per station. Two main types of synoptic situations have been identified according to the location of the pressure low generating the upper level flow, either near Portugal or near Ireland. A third type, with the pressure low above the Mediterranean, has been observed only once. The WRF-NMM simulations show that all the severe hailstorms that occurred in the twelve situations (one situation is common to both regions) developed in the areas where the mesoscale environment was characterized by a strong dynamical forcing and by high values of the low-level and deep shears. After calibration with this sample of extreme hail days, the WRF model appears promising for severe hail forecasting.

  11. Sun-Earth Day, 2001

    NASA Technical Reports Server (NTRS)

    Adams, Mitzi L.; Mortfield, P.; Hathaway, D. H.; Whitaker, Ann F. (Technical Monitor)

    2001-01-01

    To promote awareness of the Sun-Earth connection, NASA's Marshall Space Flight Center, in collaboration with the Stanford SOLAR Center, sponsored a one-day Sun-Earth Day event on April 27, 2001. Although "celebrated" on only one day, teachers and students from across the nation, prepared for over a month in advance. Workshops were held in March to train teachers. Students performed experiments, results of which were shared through video clips and an internet web cast. Our poster includes highlights from student experiments (grades 2 - 12), lessons learned from the teacher workshops and the event itself, and plans for Sun-Earth Day 2002.

  12. Modelling the tumour microenvironment in long-term microencapsulated 3D co-cultures recapitulates phenotypic features of disease progression.

    PubMed

    Estrada, Marta F; Rebelo, Sofia P; Davies, Emma J; Pinto, Marta T; Pereira, Hugo; Santo, Vítor E; Smalley, Matthew J; Barry, Simon T; Gualda, Emilio J; Alves, Paula M; Anderson, Elizabeth; Brito, Catarina

    2016-02-01

    3D cell tumour models are generated mainly in non-scalable culture systems, using bioactive scaffolds. Many of these models fail to reflect the complex tumour microenvironment and do not allow long-term monitoring of tumour progression. To overcome these limitations, we have combined alginate microencapsulation with agitation-based culture systems, to recapitulate and monitor key aspects of the tumour microenvironment and disease progression. Aggregates of MCF-7 breast cancer cells were microencapsulated in alginate, either alone or in combination with human fibroblasts, then cultured for 15 days. In co-cultures, the fibroblasts arranged themselves around the tumour aggregates creating distinct epithelial and stromal compartments. The presence of fibroblasts resulted in secretion of pro-inflammatory cytokines and deposition of collagen in the stromal compartment. Tumour cells established cell-cell contacts and polarised around small lumina in the interior of the aggregates. Over the culture period, there was a reduction in oestrogen receptor and membranous E-cadherin alongside loss of cell polarity, increased collective cell migration and enhanced angiogenic potential in co-cultures. These phenotypic alterations, typical of advanced stages of cancer, were not observed in the mono-cultures of MCF-7 cells. The proposed model system constitutes a new tool to study tumour-stroma crosstalk, disease progression and drug resistance mechanisms. PMID:26650685

  13. 5-Day repeated inhalation and 28-day post-exposure study of graphene.

    PubMed

    Shin, Jae Hoon; Han, Sung Gu; Kim, Jin Kwon; Kim, Boo Wook; Hwang, Joo Hwan; Lee, Jong Seong; Lee, Ji Hyun; Baek, Jin Ee; Kim, Tae Gyu; Kim, Keun Soo; Lee, Heon Sang; Song, Nam Woong; Ahn, Kangho; Yu, Il Je

    2015-11-01

    Graphene has recently been attracting increasing attention due to its unique electronic and chemical properties and many potential applications in such fields as semiconductors, energy storage, flexible electronics, biosensors and medical imaging. However, the toxicity of graphene in the case of human exposure has not yet been clarified. Thus, a 5-day repeated inhalation toxicity study of graphene was conducted using a nose-only inhalation system for male Sprague-Dawley rats. A total of three groups (20 rats per group) were compared: (1) control (ambient air), (2) low concentration (0.68?±?0.14?mg/m(3) graphene) and (3) high concentration (3.86?±?0.94?mg/m(3) graphene). The rats were exposed to graphene for 6?h/day for 5 days, followed by recovery for 1, 3, 7 or 28 days. The bioaccumulation and macrophage ingestion of the graphene were evaluated in the rat lungs. The exposure to graphene did not change the body weights or organ weights of the rats after the 5-day exposure and during the recovery period. No statistically significant difference was observed in the levels of lactate dehydrogenase, protein and albumin between the exposed and control groups. However, graphene ingestion by alveolar macrophages was observed in the exposed groups. Therefore, these results suggest that the 5-day repeated exposure to graphene only had a minimal toxic effect at the concentrations and time points used in this study. PMID:25697182

  14. Hispanic Culture and Relational Cultural Theory

    ERIC Educational Resources Information Center

    Ruiz, Elizabeth

    2005-01-01

    Traditional personality theories do not consider the impact of culture on personality development. Yet, to provide culturally relevant services to the increasing Hispanic population in the U.S., more culturally relevant theories must be identified. This paper presents Relational Cultural Theory (RCT) as an alternative model to understanding…

  15. Are you ready for that rainy day?

    PubMed

    O'Connor, John

    2009-01-01

    When taking out income protection, always insist that the company providing it will cover you for your 'own' occupation and not just 'any' occupation or a 'suited' one. Be aware of the deferred period or 'waiting time' and try and get some 'Day 1' in place. The younger a dentist decides to take out income protection, the better. Not only will they secure cover at a reasonable price now, but they will also be able to maintain it for their whole career. PMID:19301524

  16. Cadmium effects and accumulation in cultures of Prorocentrum micans (dinophyta)

    NASA Astrophysics Data System (ADS)

    Kayser, H.; Sperling, K.-R.

    1980-03-01

    Effects and accumulation of cadmium were studied in unialgal 10-1 batch-culture experiments with the dinoflagellate Prorocentrum micans Ehrenberg. Tests were made using sterile filtered North Sea water enriched with nitrate and phosphate only in order to avoid disturbances by complex formation. Cadmium was added to the cultures in amounts of 100 to 0.13 µg l-1. In one series it was added at the start of the experiments and in a second one after a growth period of 1 week. Addition of only 1.2 µg Cd l-1 reduces multiplication rates and maximum cell densities of the algae. Not until 0.4 µg Cd-1 does growth correspond to that of the controls. Cadmium concentrations were measured, after filtration, in the culture medium and in the biomass by means of flameless AAS. The cadmium content in algae increased from 2.7 µg g-1 (dry weight) in controls to 500 µg g-1 (dry weight) in media containing 100 µg Cd l-1. Uptake occurred rapidly during the first few days of the experiments, slowed down somewhat during exponential growth stage, and increased during decay of the cultures. Cadmium content of culture media remained nearly constant (Series 1) or decreased only slowly during experimental time (Series 2). The highest concentration factor was measured in the controls. It decreased with increasing metal concentration in the medium and increased with experimental time. Structural modifications of the cells were visible after Lugol fixation only, indicating brittleness of the cell walls. P. micans has shown to be extremely sensitive to cadmium and to accumulate this metal.

  17. Culture collections.

    PubMed

    Smith, David

    2012-01-01

    Culture collections no matter their size, form, or institutional objectives play a role in underpinning microbiology, supplying the resources for study, innovation, and discovery. Their basic roles include providing a mechanism for ex situ conservation of organisms; they are repositories for strains subject to publication, taking in safe, confidential, and patent deposits from researchers. They supply strains for use; therefore, the microorganisms provided must be authentic and preserved well, and any associated information must be valid and sufficient to facilitate the confirmation of their identity and to facilitate their use. The organisms must be collected in compliance with international conventions, international and national legislation and distributed to users indicating clearly the terms and conditions under which they are received and can be used. Collections are harmonizing approaches and characterizing strains to meet user needs. No one single collection can carry out this task alone, and therefore, it is important that output and strategy are coordinated to ensure culture collections deliver the basic resources and services microbiological innovation requires. This chapter describes the types of collection and how they can implement quality management systems and operate to deliver their basic functions. The links to information sources given not only provide support for the practitioners within collections but also provide guidance to users on accessing the huge resource available and how they can help ensure microbiology has the resources and a solid platform for future development. PMID:22569518

  18. Day Care Infection Control Protocol.

    ERIC Educational Resources Information Center

    Seattle-King County Dept. of Public Health, Seattle, WA.

    This day care infection control manual was assembled to provide technical guidance for the prevention and control of communicable diseases to child day care facilities in Seattle and King County, Washington. For each disease, the manual provides background information, public health control recommendations, and letters that can be used to…

  19. Youth Field Day Planning Guide.

    ERIC Educational Resources Information Center

    Wisconsin State Dept. of Natural Resources, Madison.

    Youth field days expose children to outdoor activities, land use ethics, and habitat conservation and encourage adults to be mentors in these areas. A typical youth field day could have programs in archery, fishing, boating, shooting, or safety. The event requires a diverse steering committee that usually includes sporting clubs and state…

  20. Day Care Center Enrichment Program.

    ERIC Educational Resources Information Center

    West Virginia State Dept. of Welfare, Charleston.

    This guide to a West Virginia Department of Welfare project for upgrading the quality of day care centers throughout the state presents samples of the forms used in the program, accompanied by a brief description of the program's format, requirements and procedures. The Day Care Center Enrichment Program provides a monetary incentive for…

  1. In Defense of Snow Days

    ERIC Educational Resources Information Center

    Goodman, Joshua

    2015-01-01

    In snowy climates, school superintendents must frequently decide whether an impending storm warrants closing schools for the day. Concerns about student and teacher safety must be weighed against the loss of student learning time, along with state requirements for days of instruction and the cost and inconvenience of extending the school year into…

  2. 2014 Maine Earth Science Day

    USGS Multimedia Gallery

    On October 15, 2014 Maine Earth Science Day was held at the Maine State Museum in Augusta. The USGS was represented by Charlie Culbertson, left, and Nick Waldron, right. This photo was taken as the two were packing up for the day, and shows a main feature of the table, a touch screen display with th...

  3. Histomorphometry and autoradiography of cultured fetal rat long bones

    SciTech Connect

    Nefussi, J.R.; Vignery, A.; Puzas, J.E.; Baron, R.

    1982-10-01

    The behavior of fetal rat long bones cultured in vitro according to Raisz's technique (1969) was studied by histomorphometry and autoradiography for a period of four days. The changes were recorded daily both on the trabecular and cortical bone by measuring the bone volume, the number of osteoclasts, and the number of nuclei per osteoclast. Radioactive calcium release was measured and compared to the changes in bone volume and in the number of osteoclasts. An autoradiographic study, using /sup 3/H-proline and /sup 3/H-thymidine in flash labeling in the medium and /sup 3/H-thymidine in follow-up labeling after one injection in vivo was performed to evaluate the bone formation, the cellular proliferation rate and cell differentiation. After four days in culture, an increase in total calcified bone volume was observed which correlated with changes in the trabecular bone. No significant changes were recorded in the cortical bone. The results showed a good maintenance of the resorption and formation phenomena through an active process of cellular multiplication and differentiation. Undifferentiated cells were labeled in flash label and osteoblast, osteocyte and some osteoclast nuclei were labeled in follow-up studies.

  4. 2011 Astronomy Day at McDonald Observatory

    NASA Astrophysics Data System (ADS)

    Preston, Sandra; Hemeway, M.; Wetzel, M.

    2012-01-01

    Our philosophy is that everyday is Astronomy Day because the McDonald Observatory's Frank N. Bash Visitors Center is open 362 days a year. So, how did we create a special celebration for the "Astronomy Day” declared by the Astronomical League? During September 26-29 we conducted 20 videoconferences and served 12,559 students with "Astronomy Day” programming. Connect2Texas provides bridging for a network of Texas-based museums and cultural, historical, and scientific organizations that offer educational content to schools throughout the state via videoconferencing. Connect2Texas connected McDonald Observatory to 334 schools; most of these schools were in Texas, but schools in a dozen other states also participated. While most schools had a "view-only" connection, at least 20 of the schools had interactive connections, whereby the students could ask questions of the presenter. Connect2Texas also collects evaluation information from the participating schools that we will use to produce a report for our funders and make modifications to future programs as need be. The videoconferences were offered free of charge. The theme for the 2011 Astronomy Day program was the Year of the Solar System, which aligns with NASA's theme for 2011 and 2012. By aligning with this NASA theme, we could leverage NASA artwork and materials to both advertise and enrich the learning experience. Videoconference materials also included pre- and post-videoconference assessment sheets, an inquiry based activity, and pre- and post-videoconference activities, all of which were made available online. One of the lessons learned from past Astronomy Day videoconferences is that the days the Astronomical League declares as "Astronomy Day” are not always good days for Texas schools to participate. So, we choose an Astronomy Day that meets the needs of Texas schools and our schedule - so any day can be Astronomy Day. 2011 Astronomy Day was made possible by The Meyer-Levy Charitable Trust.

  5. Delayed culture of Leishmania in skin biopsies.

    PubMed

    Dedet, J P; Pratlong, F; Pradinaud, R; Moreau, B

    1999-01-01

    Between January 1997 and October 1998, 16 skin biopsies collected from 13 patients with cutaneous leishmaniasis in French Guiana were inoculated in culture medium after travel for 3-17 days from the place of biopsy to the culture laboratory in France. Each biopsy fragment was introduced near the flame of a Bunsen burner into the transport medium (RPMI medium supplemented with 10% fetal calf serum) which was maintained at ambient temperature during postal delivery to France. In France the biopsies were ground in sterile saline before being inoculated into NNN culture tubes. The cultures were incubated at 25 degrees C and subcultured every week until the 5th week. The cultures were positive in 9 cases, remained negative in 4, and were contaminated in 3 cases. Positive results were obtained at all seasons and for 3 different Leishmania species. The study indicates that delayed culture can yield useful results from biopsies taken in field conditions. PMID:10717763

  6. USING ONLINE MARKETING TO INCREASE PARTICIPATION IN A WEB-BASED CONTINUING MEDICAL EDUCATION CULTURAL COMPETENCE CURRICULUM

    PubMed Central

    Estrada, Carlos A.; Krishnamoorthy, Periyakaruppan; Smith, Ann; Staton, Lisa; Korf, Michele J.; Allison, Jeroan J.; Houston, Thomas K.

    2012-01-01

    Introduction CME providers may be interested in identifying effective marketing strategies to direct users to specific content. The use of online advertisements to recruit participants for clinical trials, public health programs, and Continuing Medical Education (CME) has been shown to be effective in some but not all studies. The purpose of this study was to compare the impact of two marketing strategies in the context of an online CME cultural competence curriculum (www.c-comp.org). Methods In an interrupted time-series quasi-experimental design, two marketing strategies were tested: a) wide dissemination to relevant organizations over a period of approximately four months, and b) Internet paid search using Google Ads (five consecutive eight-week periods--control 1, cultural/ CME advertisement, control 2, hypertension/ content advertisement, control 3). Outcome measures were CME credit requests, Web traffic (visits per day, page views, pages viewed per visit), and cost. Results Overall, the site was visited 19,156 times and 78,160 pages were viewed. During the wide dissemination phase, the proportion of visits requesting CME credit decreased between the first (5.3%) and second halves (3.3%) of this phase (p= .04). During the Internet paid search phase, the proportion of visits requesting CME credit was highest during the cultural/ CME advertisement period (control 1, 1.4%; cultural/CME ad, 4.3%; control 2, 1.5%; hypertension/content ad, 0.6%; control 3, 0.8%; p<.001). All measures of Web traffic changed during the Internet paid search phase (p<.01); however, changes were independent of the advertisement periods. The incremental cost for the cultural advertisement per CME credit requested was $0.64US. Discussion Internet advertisement focusing on cultural competence and CME was associated with about a three-fold increase in requests for CME credit at an incremental cost of under $1; however, Web traffic changes were independent of the advertisement strategy. PMID:21425356

  7. Culture of hESC-derived pancreatic progenitors in alginate-based scaffolds.

    PubMed

    Formo, Kjetil; Cho, Candy H-H; Vallier, Ludovic; Strand, Berit L

    2015-12-01

    The effect of alginate-based scaffolds with added basement membrane proteins on the in vitro development of hESC-derived pancreatic progenitors was investigated. Cell clusters were encapsulated in scaffolds containing the basement membrane proteins collagen IV, laminin, fibronectin, or extracellular matrix-derived peptides, and maintained in culture for up to 46 days. The cells remained viable throughout the experiment with no signs of central necrosis. Whereas nonencapsulated cells aggregated into larger clusters, some of which showed signs of morphological changes and tissue organization, the alginate matrix stabilized the cluster size and displayed more homogeneous cell morphologies, allowing culture for long periods of time. For all conditions tested, a stable or declining expression of insulin and PDX1 and an increase in glucagon and somatostatin over time indicated a progressive reduction in beta cell-related gene expression. Alginate scaffolds can provide a chemically defined, xeno-free and easily scalable alternative for culture of pancreatic progenitors. Although no increase in insulin and PDX1 gene expression after alginate-immobilized cell culture was seen in this study, further optimization of the matrix physicochemical and biological properties and of the medium composition may still be a relevant strategy to promote the stabilization or maturation of stem cell-derived beta cells. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3717-3726, 2015. PMID:26014279

  8. The day-to-day occurrence of equatorial plasma bubbles measured from Vanimo, Papua New Guinea

    NASA Astrophysics Data System (ADS)

    Carter, B. A.; Yizengaw, E.; Francis, M.; Terkildsen, M. B.; Marshall, R. A.; Norman, R.; Zhang, K.

    2013-12-01

    An analysis of the occurrence of Equatorial Plasma Bubbles (EPBs) detected using a ground-based Global Positioning System (GPS) receiver located at Vanimo in South-east Asia will be presented. The 3-year (2000-2002) dataset employed shows that the EPB occurrence maximizes (minimizes) during the equinoxes (solstices), in good agreement with previous findings. The low-latitude ionosonde station at Vanimo is used in conjunction with the GPS receiver in an analysis of the day-to-day EPB occurrence variability during the equinox period. A superposed epoch analysis of the ionosonde data reveals that the height, and the change in height, of the F layer is 1 standard deviation (1?) larger on the days for which EPBs were detected, compared to non-EPB days. These results are interpreted using the generalized Rayleigh-Taylor (R-T) plasma instability growth rate, for which stronger upward drift of the lower-altitude F-layer plasma promotes faster growth of EPBs after sunset. These results are then compared to the results of the Thermosphere Ionosphere Electrodynamic General Circulation Model (TIEGCM), which surprisingly show strong similarities to the observations, despite only using geomagnetic and solar activity inputs. The TIEGCM is also used to directly calculate the hourly flux-tube integrated R-T growth rate. A superposed epoch analysis reveals that the modeled R-T growth rate is a little less than 1? higher on average for EPB days compared to non-EPB days. The implication of this result is that the TIEGCM generates almost enough day-to-day variability in order to account for the day-to-day EPB occurrence observed during the equinox. This result isn't necessarily expected due to the model's limited altitude coverage of 100-700 km (depending on solar activity) and the lack of ionospheric observation inputs. It is thought that the remaining variability could originate from either lower altitudes (e.g. atmospheric gravity waves from the troposphere) or from higher altitudes (resulting from coupling with the magnetosphere and solar wind), or potentially both. It is concluded that the continuing advancement of numerical modeling of the thermosphere and ionosphere, coupled with altitudes above and below, is required to better understand the day-to-day EPB occurrence.

  9. Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

    PubMed Central

    Cheng, Boran; He, Zhaobo; Zhao, Libo; Fang, Yuan; Chen, Yuanyuan; He, Rongxiang; Chen, Fangfang; Song, Haibin; Deng, Yuliang; Zhao, Xingzhong; Xiong, Bin

    2014-01-01

    Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright’s stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies. PMID:24904216

  10. Contraction-induced cluster formation in cardiac cell culture

    NASA Astrophysics Data System (ADS)

    Harada, Takahiro; Isomura, Akihiro; Yoshikawa, Kenichi

    2008-11-01

    The evolution of the spatial arrangement of cells in a primary culture of cardiac tissue derived from newborn rats was studied experimentally over an extended period. It was found that cells attract each other spontaneously to form a clustered structure over the timescale of several days. These clusters exhibit spontaneous rhythmic contraction and have been confirmed to consist of cardiac muscle cells. The addition of a contraction inhibitor (2,3-butanedione-2-monoxime) to the culture medium resulted in the inhibition of both the spontaneous contractions exhibited by the cells as well as the formation of clusters. Furthermore, the formation of clusters is suppressed when high concentrations of collagen are used for coating the substratum to which the cells adhere. From these experimental observations, it was deduced that the cells are mechanically stressed by the tension associated with repeated contractions and that this results in the cells becoming compact and attracting each other, finally resulting in the formation of clusters. This process can be interpreted as modulation of a cellular network by the activity associated with contraction, which could be employed to control cellular networks by modifying the dynamics associated with the contractions in cardiac tissue culture.

  11. Culturing the adolescent brain: what can neuroscience learn from anthropology?

    PubMed Central

    2010-01-01

    Cultural neuroscience is set to flourish in the next few years. As the field develops, it is necessary to reflect on what is meant by ‘culture’ and how this can be translated for the laboratory context. This article uses the example of the adolescent brain to discuss three aspects of culture that may help us to shape and reframe questions, interpretations and applications in cultural neuroscience: cultural contingencies of categories, cultural differences in experience and cultural context of neuroscience research. The last few years have seen a sudden increase in the study of adolescence as a period of both structural and functional plasticity, with new brain-based explanations of teenage behaviour being taken up in education, policy and medicine. However, the concept of adolescence, as an object of behavioural science, took shape relatively recently, not much more than a hundred years ago and was shaped by a number of cultural and historical factors. Moreover, research in anthropology and cross-cultural psychology has shown that the experience of adolescence, as a period of the lifespan, is variable and contingent upon culture. The emerging field of cultural neuroscience has begun to tackle the question of cultural differences in social cognitive processing in adults. In this article, I explore what a cultural neuroscience can mean in the case of adolescence. I consider how to integrate perspectives from social neuroscience and anthropology to conceptualize, and to empirically study, adolescence as a culturally variable phenomenon, which, itself, has been culturally constructed. PMID:19959484

  12. Accumulation of benzoic acid in suspension cultured cells of Pinus thunbergii Parl. in response to phenylacetic acid administration.

    PubMed

    Kawazu, K; Zhang, H; Kanzaki, H

    1996-09-01

    The generation and accumulation of both benzoic acid (BA) and its conjugates were induced in suspension cultured cells of Pinus thunbergii by administering either phenylacetic acid (PA), a toxic metabolite of Bacillus cereus (strain HY-3) accompanying the pine wood nematode, or a lyophilized culture supernatant of this bacterium. BA conjugates reached their maximal levels in quantity two days after the administration and then decreased gradually until the 14th day, while BA increased significantly throughout this period. This pattern is similar to that in 3-year-old pine trees treated with PA, suggesting that the pathological reaction of pine tissues to the PA toxin might be involved in the pathogenesis mechanism for the pine wilt disease. PMID:8987587

  13. PASSAGE OF THE MOORS DAY BY DAY ITINERARY

    E-print Network

    Toronto, University of

    . Independent Exploration: Spend the evening at leisure in Seville or relax in the hotel. DAY 3 ~ SEVILLE. Explore this vivacious city, renowned for its Flamenco music and dance as well as rich sherry and brandy

  14. Effects of Polysaccharide Elicitors on Secondary Metabolite Production and Antioxidant Response in Hypericum perforatum L. Shoot Cultures

    PubMed Central

    Gadzovska Simic, Sonja; Maury, Stéphane; Delaunay, Alain; Joseph, Claude; Hagège, Daniel

    2014-01-01

    The effects of polysaccharide elicitors such as chitin, pectin, and dextran on the production of phenylpropanoids (phenolics and flavonoids) and naphtodianthrones (hypericin and pseudohypericin) in Hypericum perforatum shoot cultures were studied. Nonenzymatic antioxidant properties (NEAOP) and peroxidase (POD) activity were also observed in shoot extracts. The activities of phenylalanine ammonia lyase (PAL) and chalcone-flavanone isomerase (CHFI) were monitored to estimate channeling in phenylpropanoid/flavonoid pathways of elicited shoot cultures. A significant suppression of the production of total phenolics and flavonoids was observed in elicited shoots from day 14 to day 21 of postelicitation. This inhibition of phenylpropanoid production was probably due to the decrease in CHFI activity in elicited shoots. Pectin and dextran promoted accumulation of naphtodianthrones, particularly pseudohypericin, within 21 days of postelicitation. The enhanced accumulation of naphtodianthrones was positively correlated with an increase of PAL activity in elicited shoots. All tested elicitors induced NEAOP at day 7, while chitin and pectin showed increase in POD activity within the entire period of postelicitation. The POD activity was in significantly positive correlation with flavonoid and hypericin contents, suggesting a strong perturbation of the cell redox system and activation of defense responses in polysaccharide-elicited H. perforatum shoot cultures. PMID:25574489

  15. Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture.

    PubMed

    Huang, Guo-Shiang; Tseng, Ting-Chen; Dai, Niann-Tzyy; Fu, Keng-Yen; Dai, Lien-Guo; Hsu, Shan-Hui

    2015-10-01

    Adipose-derived adult stem cells (ASCs) have gained much attention because of their multipotency and easy access. Here we describe a novel chitosan-based selection (CS) system instead of the conventional plastic adherence (PA) to obtain the primary ASCs. The minimal amount of adipose tissue for consistent isolation of ASCs is reduced from 10 mL to 5 mL. The selection is based on the specific interaction between cells and chitosan materials, which separate ASCs by forming spheroids during primary culture. The primary culture period was reduced from 4 days to one day and more ASCs (ten-fold expansion) were achieved in a week. The average duration for obtaining 1 × 10(7) cells takes about seven days from 5 mL of adipose tissue, compared to 14 days using the conventional PA method from 10 mL of adipose tissue. The replicative senescence of CS-ASCs is not evident until the fifteenth passage (vs. eighth for the PA-ASCs). The obtained ASCs (CS-ASCs) have less doubling time for the same passage of cells and show greater stemness than those obtained from the conventional PA method (PA-ASCs). Moreover, CS-ASCs undergo trilineage differentiation more effectively than PA-ASCs. The greater differentiation potential of CS-ASCs may be associated with the enrichment and maintenance of CD271 positive cells by chitosan selection of primary culture. PMID:26162932

  16. The Ocean Sampling Day Consortium

    SciTech Connect

    Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-Guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Gerdts, Gunnar; Polymenakou, Paraskevi; Kotoulas, Giorgos; Siam, Rania; Abdallah, Rehab Z.; Sonnenschein, Eva C.; Cariou, Thierry; O’Gara, Fergal; Jackson, Stephen; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Caçador, Isabel; Canning-Clode, João; Bobrova, Oleksandra; Marteinsson, Viggo; Reynisson, Eyjolfur; Loureiro, Clara Magalhães; Luna, Gian Marco; Quero, Grazia Marina; Löscher, Carolin R.; Kremp, Anke; DeLorenzo, Marie E.; Øvreås, Lise; Tolman, Jennifer; LaRoche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P.; Ramos, Sandra; Magalhães, Catarina; Jude-Lemeilleur, Florence; Aguirre-Macedo, Ma Leopoldina; Wang, Shiao; Poulton, Nicole; Jones, Scott; Collin, Rachel; Fuhrman, Jed A.; Conan, Pascal; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Yakimov, Michael M.; Baltar, Federico; Bodrossy, Levente; Van De Kamp, Jodie; Frampton, Dion M. F.; Ostrowski, Martin; Van Ruth, Paul; Malthouse, Paul; Claus, Simon; Deneudt, Klaas; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C.; Kandil, Mahrous M.; Amaral, Valentina; Biancalana, Florencia; Santana, Rafael; Pedrotti, Maria Luiza; Yoshida, Takashi; Ogata, Hiroyuki; Ingleton, Tim; Munnik, Kate; Rodriguez-Ezpeleta, Naiara; Berteaux-Lecellier, Veronique; Wecker, Patricia; Cancio, Ibon; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Zaid, El Houcine; Boukhatem, Noureddine; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Todorova, Nadezhda; Karamfilov, Ventzislav; ten Hoopen, Petra; Cochrane, Guy; L’Haridon, Stephane; Bizsel, Kemal Can; Vezzi, Alessandro; Lauro, Federico M.; Martin, Patrick; Jensen, Rachelle M.; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; De Pascale, Fabio; Schiavon, Riccardo; dos Santos, Antonina; Villar, Emilie; Pesant, Stéphane; Cataletto, Bruno; Malfatti, Francesca; Edirisinghe, Ranjith; Silveira, Jorge A. Herrera; Barbier, Michele; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J.; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut Cerkez; Bresnan, Eileen; Iriberri, Juan; Nyhus, Paul Anders Fronth; Bente, Edvardsen; Karlsen, Hans Erik; Golyshin, Peter N.; Gasol, Josep M.; Moncheva, Snejana; Dzhembekova, Nina; Johnson, Zackary; Sinigalliano, Christopher David; Gidley, Maribeth Louise; Zingone, Adriana; Danovaro, Roberto; Tsiamis, George; Clark, Melody S.; Costa, Ana Cristina; El Bour, Monia; Martins, Ana M.; Collins, R. Eric; Ducluzeau, Anne-Lise; Martinez, Jonathan; Costello, Mark J.; Amaral-Zettler, Linda A.; Gilbert, Jack A.; Davies, Neil; Field, Dawn; Glöckner, Frank Oliver

    2015-06-19

    In this study, Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.

  17. The Ocean Sampling Day Consortium

    DOE PAGESBeta

    Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-Guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; et al

    2015-06-19

    In this study, Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and theirmore »embedded functional traits.« less

  18. The ocean sampling day consortium.

    PubMed

    Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-Guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Gerdts, Gunnar; Polymenakou, Paraskevi; Kotoulas, Giorgos; Siam, Rania; Abdallah, Rehab Z; Sonnenschein, Eva C; Cariou, Thierry; O'Gara, Fergal; Jackson, Stephen; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Caçador, Isabel; Canning-Clode, João; Bobrova, Oleksandra; Marteinsson, Viggo; Reynisson, Eyjolfur; Loureiro, Clara Magalhães; Luna, Gian Marco; Quero, Grazia Marina; Löscher, Carolin R; Kremp, Anke; DeLorenzo, Marie E; Øvreås, Lise; Tolman, Jennifer; LaRoche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P; Ramos, Sandra; Magalhães, Catarina; Jude-Lemeilleur, Florence; Aguirre-Macedo, Ma Leopoldina; Wang, Shiao; Poulton, Nicole; Jones, Scott; Collin, Rachel; Fuhrman, Jed A; Conan, Pascal; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Yakimov, Michael M; Baltar, Federico; Bodrossy, Levente; Van De Kamp, Jodie; Frampton, Dion Mf; Ostrowski, Martin; Van Ruth, Paul; Malthouse, Paul; Claus, Simon; Deneudt, Klaas; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C; Kandil, Mahrous M; Amaral, Valentina; Biancalana, Florencia; Santana, Rafael; Pedrotti, Maria Luiza; Yoshida, Takashi; Ogata, Hiroyuki; Ingleton, Tim; Munnik, Kate; Rodriguez-Ezpeleta, Naiara; Berteaux-Lecellier, Veronique; Wecker, Patricia; Cancio, Ibon; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Zaid, El Houcine; Boukhatem, Noureddine; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Todorova, Nadezhda; Karamfilov, Ventzislav; Ten Hoopen, Petra; Cochrane, Guy; L'Haridon, Stephane; Bizsel, Kemal Can; Vezzi, Alessandro; Lauro, Federico M; Martin, Patrick; Jensen, Rachelle M; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; De Pascale, Fabio; Schiavon, Riccardo; Dos Santos, Antonina; Villar, Emilie; Pesant, Stéphane; Cataletto, Bruno; Malfatti, Francesca; Edirisinghe, Ranjith; Silveira, Jorge A Herrera; Barbier, Michele; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut Cerkez; Bresnan, Eileen; Iriberri, Juan; Nyhus, Paul Anders Fronth; Bente, Edvardsen; Karlsen, Hans Erik; Golyshin, Peter N; Gasol, Josep M; Moncheva, Snejana; Dzhembekova, Nina; Johnson, Zackary; Sinigalliano, Christopher David; Gidley, Maribeth Louise; Zingone, Adriana; Danovaro, Roberto; Tsiamis, George; Clark, Melody S; Costa, Ana Cristina; El Bour, Monia; Martins, Ana M; Collins, R Eric; Ducluzeau, Anne-Lise; Martinez, Jonathan; Costello, Mark J; Amaral-Zettler, Linda A; Gilbert, Jack A; Davies, Neil; Field, Dawn; Glöckner, Frank Oliver

    2015-01-01

    Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world's oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits. PMID:26097697

  19. Evaluation of a 3M Petrifilm on-farm culture system for the detection of intramammary infection at the end of lactation.

    PubMed

    Cameron, M; Keefe, G P; Roy, J P; Dohoo, I R; MacDonald, K A; McKenna, S L

    2013-08-01

    The purpose of this study was to evaluate a 3M Petrifilm-based on-farm culture system for the detection of intramammary infection (IMI) in low somatic cell count (SCC) cows (<200,000 cells/mL) at drying off. The main objectives were to determine the test characteristics and the predictive values of the Petrifilm on-farm culture system. The ability of dairy producers to correctly classify cows as infected or uninfected based on Petrifilm culture and a set colony count threshold was also assessed. A total of 360 cows originating from 16 low bulk tank SCC (<250,000 cells/mL) dairy herds were enrolled at drying off. Enrolled cows had an expected dry period of 30-90 days, a SCC<200,000 cells/mL on the last 3 tests prior to drying off, no clinical mastitis in the same time period, and no antibiotic treatment in the last 14 days. Quarter milk samples were collected on the day prior to drying off, and a composite milk sample was created by combining 5 mL of milk from each quarter sample. Composite milk samples were cultured on-farm using the Petrifilm culture system, which provided results within 24h. Quarter milk samples were cultured in a reference laboratory, and the results were aggregated to the cow level. On the day of drying off, the Petrifilm was read by the producer and cows were classified as positive if ?5 colonies (equivalent to 50 colony forming units/mL) were present. When read by the producer, 47.8% of the cows cultured negative on Petrifilm and were infused with only an internal teat sealant at drying off. The test characteristics of the Petrifilm on-farm culture system were calculated by comparing the producer-derived Petrifilm results to those obtained by standard laboratory culture. The sensitivity and specificity of the Petrifilm on-farm culture system were 85.2% (78.5-90.5) and 73.2% (66.4-79.3), respectively. The negative predictive value of the Petrifilm test system was high (86.6%) when estimated using the prevalence of IMI in this data set, and the positive predictive value was moderate (70.9%). An automated 3M Petrifilm reader was used to obtain accurate colony counts. The agreement between Petrifilm results obtained by the producer and those obtained by the automated Petrifilm reader was high, with a kappa value of 0.82 (0.75-0.89). PMID:23597619

  20. Spaceflight effects on cultured embryonic chick bone cells

    NASA Technical Reports Server (NTRS)

    Landis, W. J.; Hodgens, K. J.; Block, D.; Toma, C. D.; Gerstenfeld, L. C.

    2000-01-01

    A model calcifying system of primary osteoblast cell cultures derived from normal embryonic chicken calvaria has been flown aboard the shuttle, Endeavour, during the National Aeronautics and Space Administration (NASA) mission STS-59 (April 9-20, 1994) to characterize unloading and other spaceflight effects on the bone cells. Aliquots of cells (approximately 7 x 10(6)) grown in Dulbecco's modified Eagle's medium (DMEM) + 10% fetal bovine serum (FBS) were mixed with microcarrier beads, inoculated into cartridge culture units of artificial hollow fiber capillaries, and carried on the shuttle. To promote cell differentiation, cartridge media were supplemented with 12.5 microg/ml ascorbate and 10 mM beta-glycerophosphate for varying time periods before and during flight. Four cartridges contained cells from 17-day-old embryos grown for 5 days in the presence of ascorbate prior to launch (defined as flight cells committed to the osteoblastic lineage) and four cartridges supported cells from 14-day-old embryos grown for 10 days with ascorbate before launch (uncommitted flight cells). Eight cartridges prepared in the same manner were maintained under normal gravity throughout the flight (control cells) and four additional identical cartridges under normal gravity were terminated on the day of launch (basal cells). From shuttle launch to landing, all cartridges were contained in closed hardware units maintaining 5% CO2, 37 degrees C, and media delivery at a rate of approximately 1.5 ml/6 h. During day 3 and day 5 of flight, duplicate aliquots of conditioned media and accumulated cell products were collected in both the flight and the control hardware units. At the mission end, comparisons among flight, basal, and control samples were made in cell metabolism, gene expression for type I collagen and osteocalcin, and ultrastructure. Both committed and uncommitted flight cells were metabolically active, as measured by glucose uptake and lactate production, at approximately the same statistical levels as control counterparts. Flight cells elaborated a less extensive extracellular matrix, evidenced by a reduced collagen gene expression and collagen protein appearance compared with controls. Osteocalcin was expressed by all cells, a result indicating progressive differentiation of both flight and control osteoblasts, but its message levels also were reduced in flight cells compared with ground samples. This finding suggested that osteoblasts subjected to flight followed a slower progression toward a differentiated function. The summary of data indicates that spaceflight, including microgravity exposure, demonstrably affects bone cells by down-regulating type I collagen and osteocalcin gene expression and thereby inhibiting expression of the osteogenic phenotype notably by committed osteoblasts. The information is important for insight into the response of bone cells to changes of gravity and of force in general.

  1. Mathematical Aspects of the Periodic Law

    E-print Network

    Guillermo Restrepo; Leonardo A. Pachon

    2007-07-09

    We review different studies of the Periodic Law and the set of chemical elements from a mathematical point of view. This discussion covers the first attempts made in the 19th century up to the present day. Mathematics employed to study the periodic system includes number theory, information theory, order theory, set theory and topology. Each theory used shows that it is possible to provide the Periodic Law with a mathematical structure. We also show that it is possible to study the chemical elements taking advantage of their phenomenological properties, and that it is not always necessary to reduce the concept of chemical elements to the quantum atomic concept to be able to find interpretations for the Periodic Law. Finally, a connection is noted between the lengths of the periods of the Periodic Law and the philosophical Pythagorean doctrine.

  2. 'Is getting well ever an art?': Psychopharmacology and madness in Robert Lowell's Day by Day.

    PubMed

    Travis, Isabelle

    2011-12-01

    On the publication of Robert Lowell's Life Studies in 1959, some critics were shocked by the poet's use of seemingly frank autobiographical material, in particular the portrayal of his hospitalizations for bipolar disorder. During the late fifties and throughout the sixties, a rich vein, influenced by Lowell, developed in American poetry. Also during this time, the nascent science of psychopharmacology competed with and complemented the more established somatic treatments, such as psychosurgery, shock treatments, and psychoanalytical therapies. The development of Thorazine was a remarkable breakthrough allowing patients previously thought incurable to leave hospital. In 1955, the release of Miltown, the first 'minor' tranquilizer, was heralded with a media fanfare promising a new dawn of psychological cure-all. These two events blurred the boundary between 'normality' and madness by making treatment in the community more widely possible and by medicalizing more commonplace distress. Lowell's early depictions of madness situate it as emblematic of the cultural malaise of 'the tranquilized fifties.' By his final collection, Day by Day (1977), mental illness had lost its symbolic power. These late poems explore the power of art as a way of representing and remedying suffering in a culture where psychopharmacology has normalized madness. PMID:21853380

  3. Career Day - Duration: 62 seconds.

    NASA Video Gallery

    NASA's 2013 Career Days was a joint collaboration between NASA Langley and the Newport News Shipbuilding where 600 high school students from Virginia took on two design challenges -- designing a ca...

  4. Valentine's Day Party Weekday Activities

    E-print Network

    Sin, Peter

    Valentine's Day desserts. Feel free to bring food like cupcakes, cookies, or candy to share with everyone hard; hard boiled; soft boiled; poached (broken open and cooked in a little bit of water); and various

  5. EMERALD LAKE DAY HIKE Description

    E-print Network

    Lawrence, Rick L.

    EMERALD LAKE DAY HIKE Description Cost Equipment List Sign-Up Deadline You must be signed up by 5 of lodgepole pine, Engelmann spruce and subalpine r leading to Emerald Lake and Heather Lake. The lakes

  6. Beowulf, sleep, and judgment day 

    E-print Network

    Hanchey, Ginger Fielder

    2009-05-15

    , SLEP, AND JUDGMENT DAY A Thesis by GINGER SUZANE FIELDER HANCHEY Submited to the Ofice of Graduate Studies of Texas A&M University in partial fulfilment of the requirements for the degre of MASTER OF ARTS May 2008 Major Subject...: English BEOWULF, SLEP, AND JUDGMENT DAY A Thesis by GINGER SUZANE FIELDER HANCHEY Submited to the Ofice of Graduate Studies of Texas A&M University in partial fulfilment of the requirements for the degre of MASTER OF ARTS Approved...

  7. Courageous Conversations about Race: Exploring Counter-Narratives on Black Heritage Day

    ERIC Educational Resources Information Center

    Huynh, Annie

    2013-01-01

    This personal reflection explores the role of counter-narratives and courageous conversations in the elementary curriculum. It explores how the intentionality of Black Heritage Day at the Folk Arts-Cultural Treasures Charter School and its curriculum guides students and teachers in exploring issues of race, culture, and history. (Contains 1…

  8. Galileo Teacher Training Program - GTTP Days

    NASA Astrophysics Data System (ADS)

    Heenatigala, T.; Doran, R.

    2012-09-01

    Despite the vast availability of teaching resources on the internet, finding a quality and user-friendly materials is a challenge. Many teachers are not trained with proper computing skills to search for the right materials. With years of expertise training teachers globally, Galileo Teacher Training Program (GTTP) [1] recognize the need of having a go-to place for teachers to access resources. To fill this need GTTP developed - GTTP Days - a program creating resource guides for planetary, lunar and solar fields. Avoiding the imbalance in science resources between the developed and undeveloped world, GTTP Days is available both online and offline as a printable version. Each resource guide covers areas such as scientific knowledge, exploration, observation, photography, art & culture and web tools. The lesson plans of each guide include hands-on activities, web tools, software tools, and activities for people with disabilities [2]. Each activity indicate the concepts used, the skills required and age level which guides the teachers and educators to select the correct content suitable for local curriculum.

  9. A Day at the Museums

    ERIC Educational Resources Information Center

    Hubel, Joy Alter

    2009-01-01

    The school field trip, once a supporting player in a well-rounded education, is slowly becoming endangered. Widespread budget cuts have made happily anticipated class trips to museums, zoos, and other cultural destinations increasingly scarce. A librarian may be able to rescue the field trip from extinction by transforming the school building into…

  10. Time-periodic universes

    E-print Network

    De-Xing Kong; Kefeng Liu; Ming Shen

    2008-08-30

    In this letter we construct a new time-periodic solution of the vacuum Einstein's field equations whose Riemann curvature norm takes the infinity at some points. We show that this solution is intrinsically time-periodic and describes a time-periodic universe with the "black hole". New physical phenomena are investigated and new singularities are analyzed for this universal model.

  11. The Periodic Pyramid

    ERIC Educational Resources Information Center

    Hennigan, Jennifer N.; Grubbs, W. Tandy

    2013-01-01

    The chemical elements present in the modern periodic table are arranged in terms of atomic numbers and chemical periodicity. Periodicity arises from quantum mechanical limitations on how many electrons can occupy various shells and subshells of an atom. The shell model of the atom predicts that a maximum of 2, 8, 18, and 32 electrons can occupy…

  12. Gene–culture coevolution and the nature of human sociality

    PubMed Central

    Gintis, Herbert

    2011-01-01

    Human characteristics are the product of gene–culture coevolution, which is an evolutionary dynamic involving the interaction of genes and culture over long time periods. Gene–culture coevolution is a special case of niche construction. Gene–culture coevolution is responsible for human other-regarding preferences, a taste for fairness, the capacity to empathize and salience of morality and character virtues. PMID:21320901

  13. Multiwavelength periodicity study of Markarian 501

    NASA Astrophysics Data System (ADS)

    Rödig, C.; Burkart, T.; Elbracht, O.; Spanier, F.

    2009-07-01

    Context: Active galactic nuclei (AGN) are highly variable emitters of electromagnetic waves from the radio to the gamma-ray regime. This variability may be periodic, which in turn could be the signature of a binary black hole. Systems of black holes are strong emitters of gravitational waves whose amplitude depends on the binary orbital parameters such as the component mass, the orbital semi-major-axis and eccentricity. Aims: It is our aim to prove the existence of periodicity of the AGN Markarian 501 from several observations in different wavelengths. A simultaneous periodicity in different wavelengths provides evidence of bound binary black holes in the core of AGN. Methods: Existing data sets from observations by Whipple, SWIFT, RXTE, and MAGIC have been analysed with the Lomb-Scargle method, the epoch-folding technique and the SigSpec software. Results: Our analysis shows a 72-day period, which could not be seen in previous works due to the limited length of observations. This does not contradict a 23-day period that can be derived as a higher harmonic from the 72-day period.

  14. 15 CFR 904.4 - Computation of time periods.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 3 2011-01-01 2011-01-01 false Computation of time periods. 904.4... PROCEDURES General § 904.4 Computation of time periods. For a NOVA, NOPS or NIDP, the 30 day response period... in the computation....

  15. 15 CFR 904.4 - Computation of time periods.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES General § 904.4 Computation of time periods. For a NOVA, NOPS or NIDP, the 30 day response period begins to run on the date the notice is received. All other time periods begin to...

  16. 15 CFR 904.4 - Computation of time periods.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES General § 904.4 Computation of time periods. For a NOVA, NOPS or NIDP, the 30 day response period begins to run on the date the notice is received. All other time periods begin to...

  17. 15 CFR 904.4 - Computation of time periods.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES General § 904.4 Computation of time periods. For a NOVA, NOPS or NIDP, the 30 day response period begins to run on the date the notice is received. All other time periods begin to...

  18. 15 CFR 904.4 - Computation of time periods.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES General § 904.4 Computation of time periods. For a NOVA, NOPS or NIDP, the 30 day response period begins to run on the date the notice is received. All other time periods begin to...

  19. 15 CFR 904.4 - Computation of time periods.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES General § 904.4 Computation of time periods. For a NOVA, NOPS or NIDP, the 30 day response period begins to run on the date the notice is received. All other time periods begin to...

  20. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...2012-07-01 2012-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  1. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...2010-07-01 2010-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  2. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...2014-07-01 2014-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  3. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...2013-07-01 2013-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  4. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...2011-07-01 2011-07-01 false Shorter advertising periods in emergencies. 223.81...Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency...may authorize shortening the formal advertising period to not less than 7 days....

  5. 9 CFR 124.21 - Regulatory review period determination.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Regulatory review period determination... RESTORATION Regulatory Review Period § 124.21 Regulatory review period determination. (a) Not later than 30 days after the receipt of an application from PTO, APHIS shall determine the regulatory review...

  6. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...2014-07-01 2014-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  7. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...2012-07-01 2012-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  8. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...2013-07-01 2013-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  9. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...2011-07-01 2011-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  10. 38 CFR 8.6 - Calculation of time period.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...2010-07-01 2010-07-01 false Calculation of time period. 8.6 Section 8.6 Pensions...NATIONAL SERVICE LIFE INSURANCE Calculation of Time Period § 8.6 Calculation of time period. If the last day of a time...

  11. 9 CFR 124.21 - Regulatory review period determination.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Regulatory review period determination... RESTORATION Regulatory Review Period § 124.21 Regulatory review period determination. (a) Not later than 30 days after the receipt of an application from PTO, APHIS shall determine the regulatory review...

  12. 9 CFR 124.21 - Regulatory review period determination.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Regulatory review period determination... RESTORATION Regulatory Review Period § 124.21 Regulatory review period determination. (a) Not later than 30 days after the receipt of an application from PTO, APHIS shall determine the regulatory review...

  13. Antarctica Day: An International Celebration

    NASA Astrophysics Data System (ADS)

    Pope, A.; Hambrook Berkman, J.; Berkman, P. A.

    2013-12-01

    For more than half a century, the 1959 Antarctic Treaty continues to shine as a rare beacon of international cooperation. To celebrate this milestone of peace in our civilization with hope and inspiration for future generations, Antarctica Day is celebrated each year on December 1st , the anniversary of the Antarctic Treaty signing. As an annual event - initiated by the Foundation for the Good Governance of International Spaces (www.internationalspaces.org/) in collaboration with the Association of Polar Early Carer Scientists (www.apecs.is) - Antarctica Day encourages participation from around the world. The Antarctic Treaty set aside 10% of the earth, 'forever to be used exclusively for peaceful purposes in the interest of mankind.' It was the first nuclear arms agreement and the first institution to govern all human activities in an international region beyond sovereign jurisdictions. In this spirit, Antarctica Day aims to: - Demonstrate how diverse nations can work together peacefully, using science as a global language of cooperation for decision making beyond national boundaries, - Provide strategies for students learning about Antarctica through art, science and history at all school levels, - Increase collaboration and communication between classrooms, communities, researchers and government officials around the world, and - Provide a focus for polar educators to build on each year. Through close collaboration with a number of partners. Antarctica Day activities have included: a Polar Film Festival convened by The Explorers Club; live sessions connecting classrooms with scientists in Antarctica thanks to PolarTREC and ARCUS; an international activity that involved children from 13 countries who created over 600 flags which exemplify Antarctica Day (these were actually flown in Antarctica with signed certificates then returned to the classes); a map where Antarctica Day participants all over the world could share what they were doing; an Antarctic bird count involving the International Association of Antarctic Tour Operators; and public lectures and online videos (including a notable submission from Polar Educators International). Antarctica Day was initiated as a legacy of the 2009 Antarctic Treaty Summit (www.atsumit50.aq), which was convened at the Smithsonian Institution with 40 sponsoring institutions from around the world as part of the International Polar Year. Antarctic Day involved participants from 14 nations in 2010. 28 nations in 2011, and 26 nations in 2012 with representatives from all 7 continents. Antarctica Day 2013 will have recently taken place before the AGU Fall Meeting 2013, and we will present updates at that time. Our aim is to continue expanding Antarctica Day as a globally-accessible platform to share, interpret and cherish the values associated with Antarctica for the benefit of present and future generations. We look forward to the discussion and sharing that this session will provide.

  14. Culturally competent psychotherapy.

    PubMed

    Lo, Hung-Tat; Fung, Kenneth P

    2003-04-01

    To provide effective psychotherapy for culturally different patients, therapists need to attain cultural competence, which can be divided broadly into the 2 intersecting dimensions of generic and specific cultural competencies. Generic cultural competence includes the knowledge and skill set necessary to work effectively in any cross-cultural therapeutic encounter. For each phase of psychotherapy--preengagement, engagement, assessment and feedback, treatment, and termination--we discuss clinically relevant generic cultural issues under the following headings: therapist, patient, family or group, and technique. Specific cultural competence enables therapists to work effectively with a specific ethnocultural community and also affects each phase of psychotherapy. A comprehensive assessment and treatment approach is required to consider the specific effects of culture on the patient. Cultural analysis (CA) elaborates the DSM-IV cultural formulation, tailoring it for psychotherapy; it is a clinical tool developed to help therapists systematically review and generate hypotheses regarding cultural influences on the patient's psychological world. CA examines issues under 3 domains: self, relations, and treatment. We present a case to illustrate the influence of culture on patient presentation, diagnosis, CA, and psychotherapeutic treatment. Successful therapy requires therapists to employ culturally appropriate treatment goals, process, and content. The case also demonstrates various techniques with reference to culture, including countercultural, cultural reinforcing, or culturally congruent strategies and the use of contradictory cultural beliefs. In summary, developing both generic and specific cultural competencies will enhance clinician effectiveness in psychotherapy, as well as in other cross-cultural therapeutic encounters. PMID:12728740

  15. Women in Guam consume more calories during feast days than during non-feast days

    PubMed Central

    Paulino, Yvette C.; Leon Guerrero, Rachael T.; Novotny, Rachel

    2014-01-01

    Parties (feast days) have become increasingly frequent and abundant, in terms of food, on the island of Guam. Considering the potential impact of this frequency and abundance on food intake, this study compared food intake during feast days and non-feast days of women in Guam (25 Chamorros; 24 Filipinas). The women (?40 yr) recalled foods they usually consumed during feast days. Subsequently, a 24-hour dietary recall for a non-feast day was completed by a subsample (n=25). Height, weight, and waist circumference were measured to assess obesity status. Statistical analyses (paired t-test, ANOVA, and chi-square test) were performed with SPSS. Compared to a non-feast day, the women reported higher intakes of dietary energy (2645.0 ± 1125.8 versus 1654.0 ± 718.8 kcal/day), carbohydrates (43.8 ± 11.5% versus 51.8 ± 10.2% of kcal), total fat (34.1 ± 7.8% versus 27.5 ± 9.6% of kcal), saturated fat (11.4 ± 4.7% versus 7.9 ± 3.4% of kcal), and sugar (89.5 ± 62.8 versus 47.3 ± 42.2 g/day) on feast days. Chamorros, compared to Filipinas, reported higher dietary energy density (1.7 ± 0.4 versus 1.4 ± 0.3 kcal/g), total fat (35.3 ± 8.9% versus 30.7 ± 6.8% of kcal), and saturated fat (12.4 ± 4.9% versus 9.4 ± 3.3% of kcal); and lower servings of fruit (0.5 ± 1.0 versus 2.7 ± 1.8) on feast days. Fourteen Chamorros (56.0%) and one Filipina (4.1%) were classified as obese. Current feasting behaviors of women in Guam may contribute to obesity if continued for a long period. The women would benefit by choosing more fruit and vegetable dishes in place of high-energy dishes. Chamorro women would particularly benefit by reducing saturated fat intake. Traditional foods, such as taro, breadfruit, seafood, fruits, and vegetables, would help accomplish this and thus should be promoted at parties on Guam. PMID:25580033

  16. Organizational Culture and Performance

    E-print Network

    Martinez, Elizabeth A.

    Organizations are all around us. Culture is trickier—to analyze and even to see. We consider both the effect of management on culture and the effect of culture on performance. We begin by describing an intervention that ...

  17. Peritoneal fluid culture

    MedlinePLUS

    Culture - peritoneal fluid ... sent to the laboratory for Gram stain and culture. The sample is checked to see if bacteria ... based on more than just the peritoneal fluid culture (which may be negative even if you have ...

  18. Culture - joint fluid

    MedlinePLUS

    Joint fluid culture ... fungi, or viruses grow. This is called a culture. If these germs are detected, other tests may ... is no special preparation needed for the lab culture. How to prepare for the removal of joint ...

  19. Blood Culture (For Parents)

    MedlinePLUS

    ... Keeping Kids Safe Concussions: What to Know Blood Culture KidsHealth > Parents > General Health > Sick Kids > Blood Culture ... fungi has been determined. Why Do a Blood Culture? During some illnesses, certain infection-causing bacteria and ...

  20. Lymph node culture

    MedlinePLUS

    Culture - lymph node ... or viruses grow. This process is called a culture. Sometimes, special stains are also used to identify specific cells or microorganisms before culture results are available. If needle aspiration does not ...

  1. Cigarette smoke is a risk factor for severity and treatment outcome in patients with culture-positive tuberculosis

    PubMed Central

    Chuang, Hsiao-Chi; Su, Chien-Ling; Liu, Hui-Chiao; Feng, Po-Hao; Lee, Kang-Yun; Chuang, Kai-Jen; Lee, Chun-Nin; Bien, Mauo-Ying

    2015-01-01

    Objective Smoking has been associated with tuberculosis (TB); however, the effects of smoking on the effectiveness of TB treatment remain unclear. Materials and methods Data were retrieved from case notes and interviews of subjects registered in the TB-reporting system from 2010 to 2012. Study cases were defined as subjects with TB-positive sputum cultures, whereas the controls were defined as subjects with non-TB-related pulmonary diseases. Statistical analyses included logistic regression and multivariate Cox proportional hazard regression models. Results A total of 245 cases with cultures positive for TB and 114 controls with non-TB-related pulmonary diseases and negative sputum cultures were recruited. Current smokers had the highest failure rate (33%) for TB treatment, and they had the most severe pulmonary lesions based on chest X-ray grading. Current smokers had a 1.36-fold (95% confidence interval 1.03–2.36, P<0.05) higher odds ratio for cultures positive for TB compared with nonsmokers. In subjects with TB-positive cultures, current smoking was associated with an increase in treatment days required for cultures to convert from positive to negative (hazard ratio 1.12, 95% confidence interval 1.03–1.39; P<0.05). Conclusion Longer periods of treatment may be required for TB patients who are current smokers. PMID:26504395

  2. Flies remember the time of day.

    PubMed

    Chouhan, Nitin S; Wolf, Reinhard; Helfrich-Förster, Charlotte; Heisenberg, Martin

    2015-06-15

    The circadian clock enables organisms to anticipate daily environmental cycles and drives corresponding changes in behavior [1, 2]. Such endogenous oscillators also enable animals to display time-specific memory [1, 3-5]. For instance, mice and honeybees associate the location of a stimulus (like food or mate) with a certain time of day (time-place learning) [6, 7]. However, the mechanism underlying time-related learning and memory is not known. In the present study, we investigate time-specific odor learning. We use a genetically tractable animal, the fly Drosophila melanogaster. Starved flies are trained in the morning and afternoon to associate distinct odors with sucrose reward. The training is repeated the next day, and their time-dependent odor preference is tested on the third day. Our results indicate that Drosophila can express appetitive memory at the relevant time of day if the two conditioning events are separated by more than 4 hr. Flies can form time-odor associations in constant darkness (DD) as well as in a daily light-dark (LD) cycle, but not when kept under constant light (LL) conditions. Circadian clock mutants, period(01) (per(01)) and clock(AR) (clk(AR)), learned to associate sucrose reward with a certain odor but were unable to form time-odor associations. Our findings show that flies can utilize temporal information as an additional cue in appetitive learning. Time-odor learning in flies depends on a per- and clk-dependent endogenous mechanism that is independent of environmental light cues. PMID:26028434

  3. Magnetic approaches to study collective three-dimensional cell mechanics in long-term cultures (invited)

    PubMed Central

    Zhao, Ruogang; Boudou, Thomas; Wang, Wei-Gang; Chen, Christopher S.; Reich, Daniel H.

    2014-01-01

    Contractile forces generated by cells and the stiffness of the surrounding extracellular matrix are two central mechanical factors that regulate cell function. To characterize the dynamic evolution of these two mechanical parameters during tissue morphogenesis, we developed a magnetically actuated micro-mechanical testing system in which fibroblast-populated collagen microtissues formed spontaneously in arrays of microwells that each contains a pair of elastomeric microcantilevers. We characterized the magnetic actuation performance of this system and evaluated its capacity to support long-term cell culture. We showed that cells in the microtissues remained viable during prolonged culture periods of up to 15 days, and that the mechanical properties of the microtissues reached and maintained at a stable state after a fast initial increase stage. Together, these findings demonstrate the utility of this microfabricated bio-magneto-mechanical system in extended mechanobiological studies in a physiologically relevant 3D environment. PMID:24803684

  4. Dehistoricized Cultural Identity and Cultural Othering

    ERIC Educational Resources Information Center

    Weiguo, Qu

    2013-01-01

    The assumption that each culture has its own distinctive identity has been generally accepted in the discussion of cultural identities. Quite often identity formation is not perceived as a dynamic and interactive ongoing process that engages other cultures and involves change in its responses to different challenges at different times. I will…

  5. Yellow Hypergiants Show Long Secondary Periods?

    NASA Astrophysics Data System (ADS)

    Stothers, Richard B.

    2012-06-01

    There is observational evidence that intermittent long secondary periods of ~1000 days are present in the well-observed yellow hypergiants ? Cas and HR 8752. The long secondary period is interpreted here as the turnover time of giant convection cells in the convective envelope, as has been already suggested in the case of red giants and supergiants of high luminosity. The observed secondary periods and surface radial velocities of ? Cas and HR 8752 agree with the theoretical predictions, within the expected errors. These results support a theoretical interpretation that now covers the entire initial mass range from 1 to 50 M ? for luminous cool stars.

  6. YELLOW HYPERGIANTS SHOW LONG SECONDARY PERIODS?

    SciTech Connect

    Stothers, Richard B.

    2012-06-01

    There is observational evidence that intermittent long secondary periods of {approx}1000 days are present in the well-observed yellow hypergiants {rho} Cas and HR 8752. The long secondary period is interpreted here as the turnover time of giant convection cells in the convective envelope, as has been already suggested in the case of red giants and supergiants of high luminosity. The observed secondary periods and surface radial velocities of {rho} Cas and HR 8752 agree with the theoretical predictions, within the expected errors. These results support a theoretical interpretation that now covers the entire initial mass range from 1 to 50 M{sub Sun} for luminous cool stars.

  7. Day care ear surgery: our experience of 4 years.

    PubMed

    Khan, Mubarak M; Parab, Sapna R

    2012-09-01

    Day care surgery is gaining wide popularity not only in the West, but also in our country. We present our experience of 4 years of day care surgery in otological practice. To identify the scope of day care ear surgery in our rural setup and to determine the rate of complications, if any, of day care ear surgery. A prospective study of major ear cases operated in M.I.M.E.R. Medical College and Sushrut ENT Hospital was carried out from January 2005 to December 2008 with a minimum follow up of 2 years. In the period from 2005 to 2008, a total of 527 major ear cases were operated. There was re-admission of 5 operated patients (0.94%). The acceptance of day care ear surgery is enormous in our rural setup due to its obvious advantages. PMID:23998036

  8. Day-to-day variation of bronchodilatory response to an inhaled beta-2-stimulant in asthmatics.

    PubMed

    Lindgren, S; Bake, B; Larsson, S

    1987-01-01

    The effect of inhaling 0.25 and 2.0 mg of terbutaline sulphate, a beta-2-stimulant, from a metered dose aerosol was studied in five asthmatic patients during two periods of five days each. During the first period, the patients used a good spontaneous inhalation technique; during the second period, the inhalation technique was optimized and controlled. The variation of basal FEV1 and of the increase (delta FEV1) caused by 0.25 mg of inhaled terbutaline was considerable. The effect was only slightly better and the variation only slightly smaller when the controlled inhalation technique was used. The differences were not significant. In individual patients, there was no or negative correlation between delta FEV1 and the corresponding basal FEV1 value. Accordingly, the most commonly used way of expressing delta FEV1 as a percentage of basal FEV1 value was found to be insensitive. Delta FEV1, expressed as a percentage of the maximum available FEV1 increase on the same day after 2.25 mg terbutaline sulphate, was found to be most sensitive. This way of expressing delta FEV1 will increase the possibilities of detecting differences between treatments in clinical trials. PMID:3453755

  9. Sexual Hookup Culture: A Review

    PubMed Central

    Garcia, Justin R.; Reiber, Chris; Massey, Sean G.; Merriwether, Ann M.

    2013-01-01

    “Hookups,” or uncommitted sexual encounters, are becoming progressively more engrained in popular culture, reflecting both evolved sexual predilections and changing social and sexual scripts. Hook-up activities may include a wide range of sexual behaviors, such as kissing, oral sex, and penetrative intercourse. However, these encounters often transpire without any promise of, or desire for, a more traditional romantic relationship. A review of the literature suggests that these encounters are becoming increasingly normative among adolescents and young adults in North America, representing a marked shift in openness and acceptance of uncommitted sex. We reviewed the current literature on sexual hookups and considered the multiple forces influencing hookup culture, using examples from popular culture to place hooking up in context. We argue that contemporary hookup culture is best understood as the convergence of evolutionary and social forces during the developmental period of emerging adulthood. We suggest that researchers must consider both evolutionary mechanisms and social processes, and be considerate of the contemporary popular cultural climate in which hookups occur, in order to provide a comprehensive and synergistic biopsychosocial view of “casual sex” among emerging adults today. PMID:23559846

  10. Alterations in resting oxygen consumption in women exposed to 10 days of cold air

    SciTech Connect

    Armstrong, D.W.; Thomas, J.R. )

    1991-03-11

    Repeated exposure to cold air reduces the metabolic response to cold air exposure in man. The purpose of this investigation was to measure the change in resting metabolic rate (RMR) with exposure to 22C air and 4C air during a 12 day period. Four women sat in 22C air for 45 min followed by 45 min in 4C air each day for ten days. The authors measured RMR during a 45 min period in 22C air followed by 45 min in 4C air on four days. All subjects began their morning exposures on a Monday within 2 days of the onset of menses completing the study on a Friday, 12 days later. Subjects dressed in a T-shirt, shorts and cotton socks. During 45 min of exposure to warm air, RMR remained steady at 10% of VO{sub 2peak} on Day 1 and 10% on Day 5. RMR during exposure to warm air significantly increased to 13% of VO{sub 2peak} on Day 8 and remained elevated at 13% on Day 12. During exposure to cold air RMR peaked at 31% of CO{sub 2peak} by the 5th min on Day 1. Peak RMR on Day 5 was significantly lower. Pea RMR in the cold remained lower on Days 8 and 12. During cold exposure RMR peaked and then declined to steady-state during min 15 to 45. Steady-state RMR during cold exposure was significantly lower on Day 5, Day 7 and Day 12 when compared to the 23% of VO{sub 2peak} on Day 1. The authors found that RMR in cold air is significantly attenuated by Day 5 and remains lower through Day 12. RMR during warm air exposure is elevated 3% by Day 8 after five (5) days of repeated cold exposure followed by two (2) days without exposure to cold air, and RMR remains elevated on Day 12.

  11. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...exhibitor excluding time in transit; (3) Any dog or cat suffering from disease, emaciation, or injury may be destroyed by euthanasia prior to the completion of the holding period required by this section; and (4) Any live dog or cat, 120 days...

  12. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...exhibitor excluding time in transit; (3) Any dog or cat suffering from disease, emaciation, or injury may be destroyed by euthanasia prior to the completion of the holding period required by this section; and (4) Any live dog or cat, 120 days...

  13. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...exhibitor excluding time in transit; (3) Any dog or cat suffering from disease, emaciation, or injury may be destroyed by euthanasia prior to the completion of the holding period required by this section; and (4) Any live dog or cat, 120 days...

  14. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...exhibitor excluding time in transit; (3) Any dog or cat suffering from disease, emaciation, or injury may be destroyed by euthanasia prior to the completion of the holding period required by this section; and (4) Any live dog or cat, 120 days...

  15. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...exhibitor excluding time in transit; (3) Any dog or cat suffering from disease, emaciation, or injury may be destroyed by euthanasia prior to the completion of the holding period required by this section; and (4) Any live dog or cat, 120 days...

  16. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... or cat acquired by a dealer 5 or exhibitor shall be held by him or her, under his or her supervision... considered to have acquired a dog or cat which is sold through the auction sale. (1) That any live dog or cat...) Live dogs or cats which have completed a 5-day holding period with another dealer or exhibitor, or a...

  17. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... or cat acquired by a dealer 5 or exhibitor shall be held by him or her, under his or her supervision... considered to have acquired a dog or cat which is sold through the auction sale. (1) That any live dog or cat...) Live dogs or cats which have completed a 5-day holding period with another dealer or exhibitor, or a...

  18. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... or cat acquired by a dealer 5 or exhibitor shall be held by him or her, under his or her supervision... considered to have acquired a dog or cat which is sold through the auction sale. (1) That any live dog or cat...) Live dogs or cats which have completed a 5-day holding period with another dealer or exhibitor, or a...

  19. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... or cat acquired by a dealer 5 or exhibitor shall be held by him or her, under his or her supervision... considered to have acquired a dog or cat which is sold through the auction sale. (1) That any live dog or cat...) Live dogs or cats which have completed a 5-day holding period with another dealer or exhibitor, or a...

  20. 9 CFR 2.101 - Holding period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... or cat acquired by a dealer 5 or exhibitor shall be held by him or her, under his or her supervision... considered to have acquired a dog or cat which is sold through the auction sale. (1) That any live dog or cat...) Live dogs or cats which have completed a 5-day holding period with another dealer or exhibitor, or a...