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Sample records for deep phase profile

  1. Deep Seismic Reflection Profiling in Africa

    NASA Astrophysics Data System (ADS)

    Attoh, K.; Brown, L. D.

    2006-05-01

    Africa represents one of the true frontiers for systematic deep seismic reflection profiling of the type pioneered by COCORP, LITHOPROBE, BIRPS, DEKORP, and ECORS in the northern hemisphere. However, there have been a number of notable individual surveys that have sampled key components of the African lithosphere, and several systematic regional geophysical initiatives which suggest African is fertile ground for future efforts. Among the latter are the KRISP refraction/wide-angle program to probe the East African Rift system in the 1990's, the Kaapvaal Experiment to image the deep lithosphere with passive techniques and most recently the EAGLE active/passive experiments in the Afar. Examples of true multichannel deep reflection surveys to delineate crustal structure include the transects of the Limpopo Belt, a Neoarchean mobile zone that sutures the Kaapval and Zimbabwe cratons, deep oil prospecting surveys in the Nosop basin of southern Botswana that reveal dramatic basement reflectors off the NW margin of the Kaapvaal craton, and most recently deep vibroseis surveys within the Kaapvaal craton that indicate a crustal stack of tectonic slivers as well as tectonic shingling of the upper mantle. The passive margin of western Africa, with its strategic oil resources, has been a target of several deep studies using marine seismic surveys, including the PROBE initiative of the late 1980's and more recent deep surveys offshore Angola. Reprocessing of lines from oil exploration grids reveal Proterozoic mid-lower crustal features offshore of Ghana. Among the potentially rich targets for future surveys in Africa are the West African and Congo cratons and their suturing Pan-African (Neoproterozoic) mobile belts. This suite of cratonic lithosphere elements is largely largely untouched by modern high resolution seismic methodologies. New initiatives such as LEGENDS ( targeting the East African Orogen) and exploitation of existing oil industry seismic data for deep information

  2. Deep profiling of multitube flow cytometry data

    PubMed Central

    O’Neill, Kieran; Aghaeepour, Nima; Parker, Jeremy; Hogge, Donna; Karsan, Aly; Dalal, Bakul; Brinkman, Ryan R.

    2015-01-01

    Motivation: Deep profiling the phenotypic landscape of tissues using high-throughput flow cytometry (FCM) can provide important new insights into the interplay of cells in both healthy and diseased tissue. But often, especially in clinical settings, the cytometer cannot measure all the desired markers in a single aliquot. In these cases, tissue is separated into independently analysed samples, leaving a need to electronically recombine these to increase dimensionality. Nearest-neighbour (NN) based imputation fulfils this need but can produce artificial subpopulations. Clustering-based NNs can reduce these, but requires prior domain knowledge to be able to parameterize the clustering, so is unsuited to discovery settings. Results: We present flowBin, a parameterization-free method for combining multitube FCM data into a higher-dimensional form suitable for deep profiling and discovery. FlowBin allocates cells to bins defined by the common markers across tubes in a multitube experiment, then computes aggregate expression for each bin within each tube, to create a matrix of expression of all markers assayed in each tube. We show, using simulated multitube data, that flowType analysis of flowBin output reproduces the results of that same analysis on the original data for cell types of >10% abundance. We used flowBin in conjunction with classifiers to distinguish normal from cancerous cells. We used flowBin together with flowType and RchyOptimyx to profile the immunophenotypic landscape of NPM1-mutated acute myeloid leukemia, and present a series of novel cell types associated with that mutation. Availability and implementation: FlowBin is available in Bioconductor under the Artistic 2.0 free open source license. All data used are available in FlowRepository under accessions: FR-FCM-ZZYA, FR-FCM-ZZZK and FR-FCM-ZZES. Contact: rbrinkman@bccrc.ca. Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25600947

  3. Natural deep eutectic solvents: cytotoxic profile.

    PubMed

    Hayyan, Maan; Mbous, Yves Paul; Looi, Chung Yeng; Wong, Won Fen; Hayyan, Adeeb; Salleh, Zulhaziman; Mohd-Ali, Ozair

    2016-01-01

    The purpose of this study was to investigate the cytotoxic profiles of different ternary natural deep eutectic solvents (NADESs) containing water. For this purpose, five different NADESs were prepared using choline chloride as a salt, alongside five hydrogen bond donors (HBD) namely glucose, fructose, sucrose, glycerol, and malonic acid. Water was added as a tertiary component during the eutectics preparation, except for the malonic acid-based mixture. Coincidentally, the latter was found to be more toxic than any of the water-based NADESs. A trend was observed between the cellular requirements of cancer cells, the viscosity of the NADESs, and their cytotoxicity. This study also highlights the first time application of the conductor-like screening model for real solvent (COSMO-RS) software for the analysis of the cytotoxic mechanism of NADESs. COSMO-RS simulation of the interactions between NADESs and cellular membranes' phospholipids suggested that NADESs strongly interacted with cell surfaces and that their accumulation and aggregation possibly defined their cytotoxicity. This reinforced the idea that careful selection of NADESs components is necessary, as it becomes evident that organic acids as HBD highly contribute to the increasing toxicity of these neoteric mixtures. Nevertheless, NADESs in general seem to possess relatively less acute toxicity profiles than their DESs parents. This opens the door for future large scale utilization of these mixtures. PMID:27386357

  4. Deep space LADAR, phase 1

    NASA Astrophysics Data System (ADS)

    Frey, Randy W.; Rawlins, Greg; Zepkin, Neil; Bohlin, John

    1989-03-01

    A pseudo-ranging laser radar (PRLADAR) concept is proposed to provide extended range capability to tracking LADAR systems meeting the long-range requirements of SDI mission scenarios such as the SIE midcourse program. The project will investigate the payoff of several transmitter modulation techniques and a feasibility demonstration using a breadboard implementation of a new receiver concept called the Phase Multiplexed Correlator (PMC) will be accomplished. The PRLADAR concept has specific application to spaceborne LADAR tracking missions where increased CNR/SNR performance gained by the proposed technique may reduce the laser power and/or optical aperture requirement for a given mission. The reduction in power/aperture has similar cost reduction advantages in commercial ranging applications. A successful Phase 1 program will lay the groundwork for a quick reaction upgrade to the AMOS/LASE system in support of near term SIE measurement objectives.

  5. Deep seismic reflection profiling and continental growth curves

    NASA Technical Reports Server (NTRS)

    Klemperer, Simon L.

    1988-01-01

    The results of deep seismic reflection profiling is discussed which shows that the lower crust is prominently layered, in many continental areas, regardless of the age of the surface rocks. The seismic Moho is commonly shallower than the petrological Moho, leading to the question of the nature and origin of this prominent reflector in the deep crust. The lower crust is much less well defined in Phanerozoic and Proterozoic accreted terranes, suggesting possible differences in types of lower crusts.

  6. Simplified method of deep-tow seismic profiling

    USGS Publications Warehouse

    Robb, James M.; Sylwester, Richard E.; Penton, Ronald

    1981-01-01

    To improve resolution of seismic-reflection profiles in continental slope water depths of 900 to 1500 m, a single hydrophone was towed about 150 m off the bottom to receive reflected signals from a surface-towed sparker sound source. That deep-towed hydrophone data show that valleys which appear V-shaped in records from a surface-towed hydrophone are flat-bottomed, and that subbottom reflections from an erosional unconformity can be much better resolved. The data produced by this technique are very hepful when used in conjunction with records from conventional surface-towed seismic-profiling equipment.

  7. Continuous contour phase plates for tailoring the focal plane irradiance profile

    SciTech Connect

    Dixit, S.N.; Rushford, M.C.; Thomas, I.M.; Perry, M.D.

    1995-08-09

    We present fully continuous phase screens for producing super-Gaussian focal-plane irradiance profiles. Such phase screens are constructed with the assumption of either circular symmetric near-field and far-field profiles or a separable phase screen in Cartesian co-ordinates. In each case, the phase screen is only a few waves deep. Under illumination by coherent light, such phase screens produce high order super-Gaussian profiles in the focal plane with high energy content effects of beam aberrations on the focal profiles and their energy content are also discussed.

  8. Deep structures beneath Karakoram Fault revealed by deep seismic reflection profile

    NASA Astrophysics Data System (ADS)

    Lu, Z.; Gao, R.; Klemperer, S. L.; Kelly, C.

    2014-12-01

    The Karakoram fault is one of the most important structures in southwest Tibet, associated with the collision between India and Eurasia. In order to discover the deep structures of the Karakoram fault, SinoProbe has acquired a deep seismic reflection profiles with 110 km length across the Ayila Ri'gyu mountain in the southeast part of the Karakoram fault. Preliminary reflection images of the crustal structures of the Karakoram fault show that Moho reflections appear at 23 ~ 24 s (TWT) beneath deep seismic reflection line. Many north and south dipping reflections in the crust form v-shaped structures along the reflection line and suggest the Karakoram fault as a crustal scale. Beneath the Gangdese granite belt, there are many "bright-spot" reflections in the upper and middle crust

  9. Stochastic Phase Resetting: a Theory for Deep Brain Stimulation

    NASA Astrophysics Data System (ADS)

    Tass, Peter A.

    2000-03-01

    A stochastic approach to phase resetting in clusters of interacting oscillators is presented. This theory explains how a stimulus, especially a single pulse, induces synchronization and desynchronization processes. The theory is used to design a new technique for deep brain stimulation in patients suffering from Parkinson's disease or essential tremor that do no longer respond to drug therapy. This stimulation mode is a feedback controlled single pulse stimulation. The feedback signal is registered with the deep brain electrode, and the desynchronizing pulses are administered via the same electrode. The stochastic phase resetting theory is used as a starting point of a model based design of intelligent and gentle deep brain stimulation techniques.

  10. Fabrication of large aperture kinoform phase plates in fused silica for smoothing focal plane intensity profiles

    SciTech Connect

    Rushford, M.; Dixit, S.; Thomas, I.; Perry, M.

    1996-04-26

    We have fabricated large aperture (40-cm) kinoform phase plates for producing super-Gaussian focal plane intensity profiles. The continuous phase screen, designed using a new iterative procedure, was fabricated in fused silica as a 16-level, one-wave deep rewrapped phase profile using a lithographic process and wet etching in buffered hydrofluoric acid. The observed far-field contains 94% of the incident energy inside the desired spot.

  11. GAS PHASE EXPOSURE HISTORY DERIVED FROM MATERIAL PHASE CONCENTRATION PROFILES USING SOLID PHASE MICRO-EXTRACTION

    EPA Science Inventory

    EPA Identifier: F8P31059
    Title: Gas Phase Exposure History Derived from Material Phase Concentration Profiles Using Solid Phase Micro-Extraction
    Fellow (Principal Investigator): Jonathan Lewis McKinney
    Institution: University of Missouri - ...

  12. Giddings Austin chalk enters deep lean-gas phase

    SciTech Connect

    Moritis, G.

    1995-12-25

    Deep lean gas is the latest phase in the growth of the Giddings field Austin chalk play. The first phase involved drilling vertical oil and gas wells. Next came the horizontal well boom in the shallower Austin chalk area, which is still continuing. And now this third phase places horizontal laterals in the Austen chalk at about 14,000--15,000 ft to produce lean gas. The article describes the producing wells and gas gathering.

  13. Crustal structure of China from deep seismic sounding profiles

    USGS Publications Warehouse

    Li, S.; Mooney, W.D.

    1998-01-01

    More than 36,000 km of Deep Seismic Sounding (DSS) profiles have been collected in China since 1958. However, the results of these profiles are not well known in the West due to the language barrier. In this paper, we summarize the crustal structure of China with a new contour map of crustal thickness, nine representative crustal columns, and maps showing profile locations, average crustal velocity, and Pn velocity. The most remarkable aspect of the crustal structure of China is the well known 70+ km thickness of the crust of the Tibetan Plateau. The thick (45-70 km) crust of western China is separated from the thinner (30-45 km) crust of eastern China by the north-south trending seismic belt (105??E). The average crustal velocity of China ranges from 6.15 to 6.45 km/s, indicating a felsic-to-intermediate bulk crustal composition. Upper mantle (Pn) velocities are 8.0 ?? 0.2 km/s, equal to the global continental average. We interpret these results in terms of the most recent thermo-tectonic events that have modified the crust. In much of eastern China, Cenoxoic crustal extension has produced a thin crust with a low average crustal velocity, similar to western Europe and the Basin and Range Province, western USA. In western China, Mesozoic and Cenoxoic arc-continent and continent-continent collisions have led to crustal growth and thickening. Inferences on the process of crustal thickening are provided by the deep crustal velocity structure as determined by DSS profiles and other seismological studies. A high velocity (7.0-7.4 km/s) lower-crustal layer has been reported in western China only beneath the southernmost Tibetan Plateau. We identity this high-velocity layer as the cold lower crust of the subducting Indian plate. As the Indian crust is injected northward into the Tibetan lower crust, it heats and assimilates by partial melting, a process that results in a reduction in the seismic velocity of the lower crust in the central and northern Tibetan Plateau

  14. Stochastic Phase Resetting: A Theory for Deep Brain Stimulation

    NASA Astrophysics Data System (ADS)

    Tass, P. A.

    The basic principles of a stochastic approach to phase resetting in populations of interacting phase oscillators are presented in this article. This theory explains how synchronization and desynchronization processes are caused by a pulsatile stimulus. It is a central goal of this approach to establish a theoretical basis for the design of efficient and intelligent new deep brain stimulation techniques. Accordingly, the theory is used to design a new deep brain stimulation technique with feedback control in patients suffering from Parkinson's disease or essential tremor.

  15. Skeleton-migration: Applications in deep crustal reflection seismic profiling

    NASA Astrophysics Data System (ADS)

    Eaton, D. W.; Vasudevan, K.

    2009-12-01

    The reflection geometry of the sub-surface is three-dimensional in character. A 3-D seismic data acquisition and processing would be the ideal modus operandi for true seismic interpretation. However, almost all deep-crustal reflection profiles recorded on land follow quasi-linear geometry, for economic reasons. Although conventional processing of the lines accommodates crooked-line geometry, the migration algorithms used to produce seismic images for interpretation are generally 2-D in nature. Consequently, the effects of 3-D geometry are not usually well-accounted for. For example, the out-of-plane reflections lead to mislocation errors that increase with recording time. The events may be mislocated by 10’s of km and show spurious apparent dip after migration. In order to circumvent these problems and to gain insight into 3-D structures, we present an easy-to-implement “Skeleton-migration” algorithm. The skeleton-migration method follows a two-step procedure. In the first step, we introduce a fast skeletonization of the final pre-processed stack to generate a digital catalogue containing a variety of event attributes including two-way travel times and location information in UTM co-ordinates. In the second step, we apply ray-based migration to the catalogue of events or two-way travel times of the 2-D stack using an appropriate velocity model for the crust and upper mantle. Since often we do not know a priori the strike direction of the reflectors, we have implemented a fast visualization-based optimization procedure to determine the strike. In subsequent steps, we use visualization methods to view and interpret the skeleton-migration results. We illustrate the usefulness of the method with examples from both the synthetic and deep crustal seismic reflection data. For the synthetic examples, we consider physical models corresponding to a point-scatterer, a synform, a fault and a subducting slab. In all these instances, we use an elastic Kirchhoff algorithm

  16. Strike fault links mountain building from top to deep: evidence from the deep seismic reflection profiles

    NASA Astrophysics Data System (ADS)

    Gao, R.; Wang, H.; Lu, Z.; Wang, C.; Klemperer, S. L.; Yin, A.

    2013-12-01

    The formation of mountains was influenced by large-scale strike-slip faults in Tibet. At the south and north borders of the Tibetan Plateau, the Karakorum and Kunlun strike-slip faults cut the Himalayas and the Kunlun Mountains crust respectively. Based on the detection results of deep seismic reflection profiles, we report the structures of these strike-slip faults and shear deformation depth. The Karakoram fault and Indus-Yarlung suture (IYS) zone are two important structures in southwest and south Tibet, associated with the collision between India and Eurasia. SinoProbe has acquired two deep seismic reflection profiles with 210 km length. The northwestern profile spans 120 km and crosses the southeast part of the Karakoram fault where dextrally sheared mylonite and mylonitized gneiss-granite are exposed along the fault. The southeastern profile spans 90km and crosses the ophiolite belt of the western IYS. Our preliminary images show: Moho reflections appear at ~ 24 s (TWT) beneath both lines. Flower-structures imaged at the Karakoram fault zone are suggestive of strike-slip structure. There are significant differences in lower-crustal structure between the two lines. Many north and south dipping reflections in the lower crust form v-shaped structures along the northwest line. On the southeastern line, there are many north-dipping but few south-dipping reflections in the lower crust. Kunlun seismic profile crosses the active left-slip Kunlun fault, which is ~1000-km long and was inferred to merge downward with a continental subduction zone. The fault was initiated at 15-8 Ma, moved at a rate of 5-16 mm/year, and has a total slip of 65-120 km. The results of our seismic-reflection study across northeastern Tibet show that the actively deforming middle Tibetan crust is dominated by discrete sub-horizontal simple-shear zones that terminate the subvertical, left-slip Kunlun fault above. The flat shear zones appear to act as roof and floor thrusts of large duplex

  17. Saudi Arabian seismic deep-refraction profiles; final project report

    USGS Publications Warehouse

    Healy, J.H.; Mooney, W.D.; Blank, H.R.; Gettings, M.E.; Kohler, W.M.; Lamson, R.J.; Leone, L.E.

    1983-01-01

    In February 1978 a seismic deep-refraction profile was recorded by the U.S. Geological Survey along a 1000-km line across the Arabian Shield in western Saudi Arabia. The line begins in Mesozoic cover rocks near Riyadh on the Arabian Platform, leads southwesterly across three major Precambrian tectonic provinces, traverses Cenozoic rocks of the coastal plain near Jizan (Tihamat-Asir), and terminates at the outer edge of the Farasan Bank in the southern Red Sea. More than 500 surveyed recording sites were occupied, including 19 in the Farasan Islands. Six shot points were used: five on land, with most charges placed below the water table in drill holes, and one at sea, with charges placed on the sea floor and detonated from a ship. Slightly more than 61 metric tons of explosives were used in 19 discrete firings. Seismic energy was recorded by 100 newly-developed portable seismic stations deployed in approximately 200 km-long arrays for each firing. Each station consisted of a standard 2-Hz vertical component geophone coupled to a self-contained analog recording instrument equipped with a magnetic-tape cassette. In this final report, we fully document the field and data-processing procedures and present the final seismogram data set as both a digital magnetic tape and as record sections for each shot point. Record sections include a normalized set of seismograms, reduced at 6 km/s, and a true-amplitude set, reduced at 8 km/s, which have been adjusted for amplifier gain, individual shot size, and distance from the shot point. Appendices give recorder station and shot information, digital data set descriptions, computer program listings, arrival times used in the interpretation, and a bibliography of reports published as a result of this project. We used two-dimensional ray-tracing techniques in the data analysis, and our interpretation is based primarily on horizontally layered models. The Arabian Shield is composed, to first-order, of two layers, each about 20 km

  18. Deep seismic profiling using off-line recorders, Miura Peninsula, Kanto, Japan.

    NASA Astrophysics Data System (ADS)

    Kawamura, T.; Hirata, N.; Sato, H.; Onishi, M.; Noda, K.

    2003-12-01

    The deep seismic profiling around Metropolitan Tokyo (Kanto area) began from 2002 under the project named . The deep seismic profiling was performed along the Tokyo Bay (Tokyo Bay 2003) from July to August in 2003, to obtain an image of the source fault of the Kanto earthquake of 1923 (M7.9), and deeper extension of inland active faults in Miura Peninsula. In the Tokyo Bay 2003, seismic reflection data were acquired along a 71-km-long seismic line from the Miura Peninsula to Tokyo Bay, using digital-telemetry cable system, including ocean bottom cables. However, this seismic line at Miura Peninsula passes through the densely populated area and due to the cultural noise the low S/N ratio was expected. Keeping away from the noise along the cable-type receiver line, 51 off-line recorders with a 4.5 Hz and 3-compornent geophone were deployed at carefully selected, quiet receiver points. During 90 days continuous recording, seismic signals produced by four vibroseis trucks at 195 locations and air-guns (1500 cu. inch) at 4280 locations were recorded including ca. 150 of earthquakes. Judging from the preliminary receiver gathers, two later phases (A and B) are recognized. The later phase A, located at TWT (Two-way travel time) 4 to 5.5 sec., is interpreted as a reflection from the top of the Philippine Sea Plate. The later phase B at TWT 7 to 8 sec. corresponds to the deeper reflection under the Philippine Sea Plate, respectively.

  19. Deep Structure of the NE Tibetan Plateau: An Introduction to Project INDEPTH, Phase IV

    NASA Astrophysics Data System (ADS)

    Zhao, W.; Brown, L.; Zhenhan, W.; Klemperer, S.; Shi, D.; Mechie, J.; Su, H.; Tilmann, F.; Guangqi, X.; Karplus, M.; Kind, R.; Makovsky, Y.; Meissner, R.

    2007-12-01

    Since 1992, INDEPTH has acquired active and passive seismic data in three major experiments from south of the High Himalaya to the Qiangtang terrane, north of the Banggong suture. Phase IV of Project INDEPTH is focussed on delineating deep crustal and mantle structure beneath the northeast margin of the Tibetan Plateau, thought by many to represent the focus of active growth of the plateau into the Asian continent. Primary scientific objectives include: i) elucidating the crustal geometry of the thrust/strike slip fault system of the Kunlun that marks the edge of the high plateau, ii) testing the controversial suggestion that the Asian continental lithosphere is underthrusting southwestward beneath the Tibetan Plateau beneath the Kunlun, iii) assessing the role of partial melting in the tectonics of NE Tibet, and contraints on postulated crustal flow. The major technical components of this year's work include: 1) Active seismic imaging with near-vertical and wide-angle techniques; 2) Passive seismic profiling using dense seismometer spacing (ca 5 km) designed to produce high resolution receiver-function images of lithospheric structure; and 3) Geological surface investigations. Additional components scheduled for next year include: 1) a high resolution gravity survey along the seismic profile, and 2) wideband MT and long-period MT ( LIMS ) measurements to investigate deep electrical conductivity along the seismic profiles. The INDEPTH IV controlled source profile extends from Qarhan Salt Lake in the center of the Qaidam basin, across the Kunlun mountains of northern Tibet to Quemalai near the Chumaer River. The INDEPTH IV passive- source profiles are collocated with the controlled source profile across the Kunlun, but includes a second high resolution profile across the Jinsha Suture on the Tibetan Plateau. The INDEPTH IV geological studies are focussed on the timing of crustal shortening and uplift of the Kunlun and adjacent Plateau. Preliminary results will be

  20. Deep Space Habitat Team: HEFT Phase 2 Effects

    NASA Technical Reports Server (NTRS)

    Toups, Larry D.; Smitherman, David; Shyface, Hilary; Simon, Matt; Bobkill, Marianne; Komar, D. R.; Guirgis, Peggy; Bagdigian, Bob; Spexarth, Gary

    2011-01-01

    HEFT was a NASA-wide team that performed analyses of architectures for human exploration beyond LEO, evaluating technical, programmatic, and budgetary issues to support decisions at the highest level of the agency in HSF planning. HEFT Phase I (April - September, 2010) and Phase II (September - December, 2010) examined a broad set of Human Exploration of Near Earth Objects (NEOs) Design Reference Missions (DRMs), evaluating such factors as elements, performance, technologies, schedule, and cost. At end of HEFT Phase 1, an architecture concept known as DRM 4a represented the best available option for a full capability NEO mission. Within DRM4a, the habitation system was provided by Deep Space Habitat (DSH), Multi-Mission Space Exploration Vehicle (MMSEV), and Crew Transfer Vehicle (CTV) pressurized elements. HEFT Phase 2 extended DRM4a, resulting in DRM4b. Scrubbed element-level functionality assumptions and mission Concepts of Operations. Habitation Team developed more detailed concepts of the DSH and the DSH/MMSEV/CTV Conops, including functionality and accommodations, mass & volume estimates, technology requirements, and DDT&E costs. DRM 5 represented an effort to reduce cost by scaling back on technologies and eliminating the need for the development of an MMSEV.

  1. Simple and Integrated Spintip-Based Technology Applied for Deep Proteome Profiling.

    PubMed

    Chen, Wendong; Wang, Shuai; Adhikari, Subash; Deng, Zuhui; Wang, Lingjue; Chen, Lan; Ke, Mi; Yang, Pengyuan; Tian, Ruijun

    2016-05-01

    Great efforts have been taken for developing high-sensitive mass spectrometry (MS)-based proteomic technologies, among which sample preparation is one of the major focus. Here, a simple and integrated spintip-based proteomics technology (SISPROT) consisting of strong cation exchange beads and C18 disk in one pipet tip was developed. Both proteomics sample preparation steps, including protein preconcentration, reduction, alkylation, and digestion, and reversed phase (RP)-based desalting and high-pH RP-based peptide fractionation can be achieved in a fully integrated manner for the first time. This easy-to-use technology achieved high sensitivity with negligible sample loss. Proteomic analysis of 2000 HEK 293 cells readily identified 1270 proteins within 1.4 h of MS time, while 7826 proteins were identified when 100000 cells were processed and analyzed within only 22 h of MS time. More importantly, the SISPROT can be easily multiplexed on a standard centrifuge with good reproducibility (Pearson correlation coefficient > 0.98) for both single-shot analysis and deep proteome profiling with five-step high-pH RP fractionation. The SISPROT was exemplified by the triplicate analysis of 100000 stem cells from human exfoliated deciduous teeth (SHED). This led to the identification of 9078 proteins containing 3771 annotated membrane proteins, which was the largest proteome data set for dental stem cells reported to date. We expect that the SISPROT will be well suited for deep proteome profiling for fewer than 100000 cells and applied for translational studies where multiplexed technology with good label-free quantification precision is required. PMID:27062885

  2. Comparing vertical profiles of natural tracers in the Williston Basin to estimate the onset of deep aquifer activation

    NASA Astrophysics Data System (ADS)

    Hendry, M. Jim; Harrington, Glenn A.

    2014-08-01

    Comparing high-resolution depth profiles of different naturally occurring environmental tracers in aquitards should yield consistent and perhaps complementary information about solute transport mechanisms and the timing of major hydrogeological and climatological events. This study evaluated whether deep, continuous profiles of aquitard pore water chloride concentration could provide further insight into the paleohydrology of the Williston Basin, Canada, than possible using high-resolution depth profiles of stable H/O isotopes of water (δ18O, δ2H). Pore water samples were obtained from extracts of cores taken over 392 m of the thick Cretaceous shale aquitard. Water samples were also collected from wells installed in the underlying regional sandy aquifer (Mannville Group; 93 m thick) and from seepage inflows into potash mine shafts (to 825 m below ground). Numerical modeling of the 1-D vertical Cl- profile supported diffusion dominated solute transport in the shales. The modeling also showed a similar time frame for development of the Cl- profile prior to activation of the aquifer as determined from the δ18O profile (20-25 Ma); however, it provided a significantly longer and potentially better-constrained time frame for evolution of the profile during the activation phase of the aquifer (0.5-1 Ma). The dominant paleoevent reflected in present-day profiles of both tracers is the introduction of glaciogenic meteoric water to the Mannville aquifer underlying the shale during the Pleistocene. The source area of this water remains to be determined.

  3. Phase 2 Methyl Iodide Deep-Bed Adsorption Tests

    SciTech Connect

    Soelberg, Nick; Watson, Tony

    2014-09-01

    Nuclear fission produces fission products (FPs) and activation products, including iodine-129, which could evolve into used fuel reprocessing facility off-gas systems, and could require off-gas control to limit air emissions to levels within acceptable emission limits. Research, demonstrations, and some reprocessing plant experience have indicated that diatomic iodine can be captured with efficiencies high enough to meet regulatory requirements. Research on the capture of organic iodides has also been performed, but to a lesser extent. Several questions remain open regarding the capture of iodine bound in organic compounds. Deep-bed methyl iodide adsorption testing has progressed according to a multi-laboratory methyl iodide adsorption test plan. This report summarizes the second phase of methyl iodide adsorption work performed according to this test plan using the deep-bed iodine adsorption test system at the Idaho National Laboratory (INL), performed during the second half of Fiscal Year (FY) 2014. Test results continue to show that methyl iodide adsorption using AgZ can achieve total iodine decontamination factors (DFs, ratios of uncontrolled and controlled total iodine levels) above 1,000, until breakthrough occurred. However, mass transfer zone depths are deeper for methyl iodide adsorption compared to diatomic iodine (I2) adsorption. Methyl iodide DFs for the Ag Aerogel test adsorption efficiencies were less than 1,000, and the methyl iodide mass transfer zone depth exceeded 8 inches. Additional deep-bed testing and analyses are recommended to (a) expand the data base for methyl iodide adsorption under various conditions specified in the methyl iodide test plan, and (b) provide more data for evaluating organic iodide reactions and reaction byproducts for different potential adsorption conditions.

  4. Seeking the Profile of an Elementary Educator: Phase III.

    ERIC Educational Resources Information Center

    Arth, Alfred A.; And Others

    This paper presents the third phase of a student-faculty investigation seeking the profile of the elementary school teacher. Phase I discovered an indication of different personality traits in elementary and secondary teachers. Phase II redesigned the original questionnaire and supported the findings with additional research. This third phase…

  5. PROTEOFORMER: deep proteome coverage through ribosome profiling and MS integration

    PubMed Central

    Crappé, Jeroen; Ndah, Elvis; Koch, Alexander; Steyaert, Sandra; Gawron, Daria; De Keulenaer, Sarah; De Meester, Ellen; De Meyer, Tim; Van Criekinge, Wim; Van Damme, Petra; Menschaert, Gerben

    2015-01-01

    An increasing amount of studies integrate mRNA sequencing data into MS-based proteomics to complement the translation product search space. However, several factors, including extensive regulation of mRNA translation and the need for three- or six-frame-translation, impede the use of mRNA-seq data for the construction of a protein sequence search database. With that in mind, we developed the PROTEOFORMER tool that automatically processes data of the recently developed ribosome profiling method (sequencing of ribosome-protected mRNA fragments), resulting in genome-wide visualization of ribosome occupancy. Our tool also includes a translation initiation site calling algorithm allowing the delineation of the open reading frames (ORFs) of all translation products. A complete protein synthesis-based sequence database can thus be compiled for mass spectrometry-based identification. This approach increases the overall protein identification rates with 3% and 11% (improved and new identifications) for human and mouse, respectively, and enables proteome-wide detection of 5′-extended proteoforms, upstream ORF translation and near-cognate translation start sites. The PROTEOFORMER tool is available as a stand-alone pipeline and has been implemented in the galaxy framework for ease of use. PMID:25510491

  6. Phase error compensation methods for high-accuracy profile measurement

    NASA Astrophysics Data System (ADS)

    Cai, Zewei; Liu, Xiaoli; Peng, Xiang; Zhang, Zonghua; Jiang, Hao; Yin, Yongkai; Huang, Shujun

    2016-04-01

    In a phase-shifting algorithm-based fringe projection profilometry, the nonlinear intensity response, called the gamma effect, of the projector-camera setup is a major source of error in phase retrieval. This paper proposes two novel, accurate approaches to realize both active and passive phase error compensation based on a universal phase error model which is suitable for a arbitrary phase-shifting step. The experimental results on phase error compensation and profile measurement of standard components verified the validity and accuracy of the two proposed approaches which are robust when faced with changeable measurement conditions.

  7. Deep Temperature Profiles to Evaluate Recharge in the Desert Southwest, United States

    NASA Astrophysics Data System (ADS)

    Dowman, C. E.; Dowman, C. E.; Hoffmann, J. P.; Ferre', P. A.

    2001-12-01

    Natural recharge is one of the most poorly constrained aspects of hydrologic budgets in the southwestern United States. One component of natural recharge to alluvial aquifers in many basins in the desert Southwest occurs through infiltration beneath ephemeral stream channels. Shallow temperature methods have been successfully used to estimate recharge beneath these channels. These methods rely on variations in shallow (less than 5 meters) temperature changes, which are due to advective flow of heat from infiltrating water, and are often applied in areas where depth to ground water is shallow. In cases where shallow temperature methods are used in thick unsaturated zones, it must be assumed that flux beyond the depth of the measurement is equivalent to recharge. For thick unsaturated zones, deep temperature profiles can be used to estimate recharge. This method relies on the alteration of temperature profiles deeper in unsaturated zones by percolating water. Reduced gradients deep beneath active channels indicate downward heat transfer by percolation of water. This heat transfer perturbs the geothermal gradient, resulting in a heat deficit in the vertical profile. This investigation uses deep temperature profiles below stream channels for analysis of the effects of percolation on the geothermal gradient. Recharge estimates made on the basis of heat deficits are compared with recharge estimates made using shallow-temperature methods. Utility of the deep temperature-profile method in areas where shallow-temperature methods may not be appropriate is examined. The analysis evaluates temperature profiles from boreholes within and adjacent to ephemeral stream channels in the Sonoran and Mojave Deserts.

  8. Phase 1 Methyl Iodide Deep-Bed Adsorption Tests

    SciTech Connect

    Nick Soelberg; Tony Watson

    2014-08-01

    Nuclear fission results in the production of fission products (FPs) and activation products including iodine-129, which could evolve into used fuel reprocessing facility off-gas systems, and could require off-gas control to limit air emissions to levels within acceptable emission limits. Research, demonstrations, and some reprocessing plant experience have indicated that diatomic iodine can be captured with efficiencies high enough to meet regulatory requirements. Research on the capture of organic iodides has also been performed, but to a lesser extent [Jubin 2012b]. Several questions remain open regarding the capture of iodine bound in organic compounds. Deep-bed methyl iodide adsorption testing has progressed according to a multi-laboratory methyl iodide adsorption test plan. This report summarizes the first phase of methyl iodide adsorption work performed according to this test plan using the deep-bed iodine adsorption test system at the Idaho National Laboratory (INL), performed during Fiscal Year (FY) 2013 and early FY-2014. Testing has been performed to address questions posed in the test plan, and followed the testing outline in the test plan. Tests established detection limits, developed procedures for sample analysis with minimal analytical interferences, and confirmed earlier results that show that the methyl iodide reacts when in contact with the AgZ sorbent, and not significantly in the gas flow upstream of the sorbent. The reaction(s) enable separation of the iodine from the organic moiety, so that the iodine can chemisorb onto the sorbent. The organic moiety can form other compounds, some of which are organic compounds that are detected and can be tentatively identified using GC-FID and GCMS. Test results also show that other gas constituents (NOx and/or H2O) can affect the methyl iodide reactions. With NOx and H2O present in the gas stream, the majority of uncaptured iodine exiting iodine-laden sorbent beds is in the form of I2 or HI, species that

  9. Reverse Phase Protein Arrays for Compound Profiling.

    PubMed

    Moerke, Nathan; Fallahi-Sichani, Mohammad

    2016-01-01

    Reverse phase protein arrays (RPPAs), also called reverse phase lysate arrays (RPLAs), involve immobilizing cell or tissue lysates, in small spots, onto solid supports which are then probed with primary antibodies specific for proteins or post-translational modifications of interest. RPPA assays are well suited for large-scale, high-throughput measurement of protein and PTM levels in cells and tissues. RPPAs are affordable and highly multiplexable, as a large number of arrays can readily be produced in parallel and then probed separately with distinct primary antibodies. This article describes a procedure for treating cells and preparing cell lysates, as well as a procedure for generating RPPAs using these lysates. A method for probing, imaging, and analyzing RPPAs is also described. These procedures are readily adaptable to a wide range of studies of cell signaling in response to drugs and other perturbations. © 2016 by John Wiley & Sons, Inc. PMID:27622568

  10. Afghanistan environmental profile. Phase 1. Final report

    SciTech Connect

    Not Available

    1992-06-01

    Afghanistan's environment, already scarred by 12 years of conflict, is likely to undergo severe stress as external and internal refugees are resettled, according to this preliminary environmental profile. Following an introduction, Chapter 2 discusses the state of Afghanistan's environment in 1978 prior to the Soviet invasion, while Chapter 3 documents the environmental impacts of events since that time, including population relocation, deforestation, and locust and sunn-pest infestations. Chapter 4 examines major environmental areas (vegetation, wildlife, soil erosion, pesticides, public health, environmental infrastructure, energy, and air quality) with respect to both existing conditions and what is likely to occur when resettlement begins in earnest. Chapter 5 presents potential mitigation measures, including a set of environmental guidelines for the Government of Afghanistan. Chapter 6 discusses the Geographic Information System being developed under USAID's Agricultural Services Support Program; it discusses the extent to which GIS data can contribute to environmental studies, and vice versa.

  11. Temporal profile of improvement of tardive dystonia after globus pallidus deep brain stimulation

    PubMed Central

    Shaikh, Aasef G.; Mewes, Klaus; DeLong, Mahlon R.; Gross, Robert E.; Triche, Shirley D.; Jinnah, H.A.; Boulis, Nicholas; Willie, Jon T.; Freeman, Alan; Alexander, Garrett E.; Aia, Pratibha; Butefisch, Cathrine M.; Esper, Christine D.; Factor, Stewart A.

    2016-01-01

    Background Several case reports and small series have indicated that tardive dystonia is responsive to globus pallidus deep brain stimulation. Whether different subtypes or distributions of tardive dystonia are associated with different outcomes remains unknown. Methods We assessed the outcomes and temporal profile of improvement of eight tardive dystonia patients who underwent globus pallidus deep brain stimulation over the past six years through record review. Due to the retrospective nature of this study, it was not blinded or placebo controlled. Results: Consistent with previous studies, deep brain stimulation improved the overall the Burkee–Fahn–Marsden motor scores by 85.1 ± 13.5%. The distributions with best responses in descending order were upper face, lower face, larynx/pharynx, limbs, trunk, and neck. Patients with prominent cervical dystonia demonstrated improvement in the Toronto Western Spasmodic Torticollis Rating Scale but improvements took several months. In four patients the effects of deep brain stimulation on improvement in Burke Fahn Marsden score was rapid, while in four cases there was partial rapid response of neck and trunk dystonia followed by was gradual resolution of residual symptoms over 48 months. Conclusion Our retrospective analysis shows excellent resolution of tardive dystonia after globus pallidus deep brain stimulation. We found instantaneous response, except with neck and trunk dystonia where partial recovery was followed by further resolution at slower rate. Such outcome is encouraging for using deep brain stimulation in treatment of tardive dystonia. PMID:25465373

  12. Mantle phase changes and deep-earthquake faulting in subducting lithosphere

    USGS Publications Warehouse

    Kirby, S.H.; Durham, W.B.; Stern, L.A.

    1991-01-01

    Inclined zones of earthquakes are the primary expression of lithosphere subduction. A distinct deep population of subduction-zone earthquakes occurs at depths of 350 to 690 kilometers. At those depths ordinary brittle fracture and frictional sliding, the faulting processes of shallow earthquakes, are not expected. A fresh understanding of these deep earthquakes comes from developments in several areas of experimental and theoretical geophysics, including the discovery and characterization of transformational faulting, a shear instability connected with localized phase transformations under nonhydrostatic stress. These developments support the hypothesis that deep earthquakes represent transformational faulting in a wedge of olivine-rich peridotite that is likely to persist metastably in coldest plate interiors to depths as great as 690 km. Predictions based on this deep structure of mantle phase changes are consistent with the global depth distribution of deep earthquakes, the maximum depths of earthquakes in individual subductions zones, and key source characteristics of deep events.

  13. Mantle phase changes and deep-earthquake faulting in subducting lithosphere.

    PubMed

    Kirby, S H; Durham, W B; Stern, L A

    1991-04-12

    Inclined zones of earthquakes are the primary expression of lithosphere subduction. A distinct deep population of subduction-zone earthquakes occurs at depths of 350 to 690 kilometers. At those depths ordinary brittle fracture and frictional sliding, the faulting processes of shallow earthquakes, are not expected. A fresh understanding of these deep earthquakes comes from developments in several areas of experimental and theoretical geophysics, including the discovery and characterization of transformational faulting, a shear instability connected with localized phase transformations under nonhydrostatic stress. These developments support the hypothesis that deep earthquakes represent transformational faulting in a wedge of olivine-rich peridotite that is likely to persist metastably in coldest plate interiors to depths as great as 690 km. Predictions based on this deep structure of mantle phase changes are consistent with the global depth distribution of deep earthquakes, the maximum depths of earthquakes in individual subductions zones, and key source characteristics of deep events. PMID:17769266

  14. Structure of Precambrian crust in the U. S. from COCORP deep seismic profiling

    SciTech Connect

    Brown, L.D. )

    1992-01-01

    COCORP and industry seismic reflection profiles probing beneath the thin veneer of Paleozoic sedimentary rocks of the US mid-continent are mapping a complex, largely unknown three dimensional mosaic of major fault zones and sutures, a highly variable Moho, and extensive sequences of unexplored volcanic and/or sedimentary strata. Key features of the Precambrian suggested by COCORP and other deep profiling include: Pervasive, distributed reflectivity, often diffractive, dominating the middle and lower crust. Moho that is rarely reflective, usually evident as a downward transition of distributed crustal reflectivity into mantle transparency. Volcano-clastic filled graben of the late Proterozoic Keweenawan rift buried beneath Paleozoic strata in Kansas and Michigan. Extensive, subhorizontal Precambrian stratification in the upper crust beneath the east- central US and the Texas-Oklahoma border region, argued to be either an extensive volcano-clastic basin, a voluminous felsic volcanic outpouring or a major intrusive sill complex. Crustal penetrating, dipping reflection zones that mark known (Grenville front) or inferred (Cashocton zone, Trans-Hudson orogen) shear zones. Non-reflective ( ) basement beneath the Appalachian foreland suggesting transparent massifs'' that serve as collisional buttresses during terrane accretion. Deep structure is sometimes at odds with simple extrapolations of surface geology. Clearly deep seismic profiling has only begun to reveal the buried craton in the US. It is time for an integrated program for the systematic exploration of this special scientific frontier.

  15. Investigation of deep implanted fluorine channeling profiles in silicon using resonant NRA

    NASA Astrophysics Data System (ADS)

    Kokkoris, M.; Perdikakis, G.; Vlastou, R.; Papadopoulos, C. T.; Aslanoglou, X. A.; Posselt, M.; Grötzschel, R.; Harissopulos, S.; Kossionides, S.

    2003-04-01

    Si(1 0 0) and (1 1 1) crystals were irradiated in the random as well as in the channeling direction, using 5 MeV 19F + ions, to a maximum fluence of approximately 1×10 17 particles/cm 2. The occurring deep implanted profiles were subsequently investigated using the Resonant Nuclear Reaction Analysis technique in the energy range Ep=950-1200 keV. The reaction 19F(p,αγ) 16O reaction exhibits a strong resonant behavior in the above mentioned energy range, thus providing an excellent tool for the depth profiling of fluorine, yielding minimum detection limits of the order of a few ppm. The occurring profiles are analyzed with SRIM and c-TRIM codes and an attempt is made to explain the characteristics of the experimental spectra, as well as to compare with results already existing in literature.

  16. Deep Space Habitat Concept of Operations for Transit Mission Phases

    NASA Technical Reports Server (NTRS)

    Hoffman, Stephen J.

    2011-01-01

    The National Aeronautics and Space Administration (NASA) has begun evaluating various mission and system components of possible implementations of what the U.S. Human Spaceflight Plans Committee (also known as the Augustine Committee) has named the flexible path (Anon., 2009). As human spaceflight missions expand further into deep space, the duration of these missions increases to the point where a dedicated crew habitat element appears necessary. There are several destinations included in this flexible path a near Earth asteroid (NEA) mission, a Phobos/Deimos (Ph/D) mission, and a Mars surface exploration mission that all include at least a portion of the total mission in which the crew spends significant periods of time (measured in months) in the deep space environment and are thus candidates for a dedicated habitat element. As one facet of a number of studies being conducted by the Human Spaceflight Architecture Team (HAT) a workshop was conducted to consider how best to define and quantify habitable volume for these future deep space missions. One conclusion reached during this workshop was the need for a description of the scope and scale of these missions and the intended uses of a habitat element. A group was set up to prepare a concept of operations document to address this need. This document describes a concept of operations for a habitat element used for these deep space missions. Although it may eventually be determined that there is significant overlap with this concept of operations and that of a habitat destined for use on planetary surfaces, such as the Moon and Mars, no such presumption is made in this document.

  17. Deep seismic soundings on the 1-AP profile in the Barents Sea: Methods and results

    NASA Astrophysics Data System (ADS)

    Sakoulina, T. S.; Kashubin, S. N.; Pavlenkova, G. A.

    2016-07-01

    Profile 1-AP with a length of 1300 km intersects the Barents Sea from The Kola Peninsula to Franz Josef Land. The combined Common Depth Point (CDP) and Deep Seismic Sounding (DSS) seismic studies were carried out on this profile. The DSS measurements were conducted with the standalone bottom seismic stations with an interval of 5-20 km between them. The stations recorded the signals generated by the large air guns with a step of 250 m. Based on these data, the detailed P-velocity section of the Earth's crust and uppermost mantle have been constructed for the entire profile and the S-velocity section for its southern part. The use of a variety of methods for constructing the velocity sections enabled us to assess the capabilities of each method from the standpoint of the highest reliability and informativity of the models. The ray tracing method yielded the best results. The 1-PR profile crosses two large basins—the South Barents and North Barents ones, with the thickness of the sediments increasing from 8 to 10 km in the south to 12-15 km in the north. The Earth's crust pertains to the continental type along the entire profile. Its thickness averages 32 to 36 km and only increases to 43 km at the boundary between the two basins. The distinct change in the wave field at this boundary suggests the presence of a large deep fault in this zone. The high-velocity blocks are revealed in the crust of the South Barents basin, whereas the North Barents crust is characterized by relatively low velocities.

  18. Gas-phase exposure history derived from material-phase concentration profiles

    NASA Astrophysics Data System (ADS)

    Morrison, G. C.; Little, J. C.; Xu, Y.; Rao, M.; Enke, D.

    Non-reactive gas-phase pollutants such as benzene diffuse into indoor furnishings and leave behind a unique material-phase concentration profile that serves as a record of the past gas-phase indoor concentrations. The inverse problem to be solved is the diffusion equation in a slab such as vinyl flooring. Using knowledge of the present material-phase concentration profile in the slab, we seek to determine the historical material-phase concentration at the surface exposed to indoor air, and hence the historical gas-phase concentration, which can be used directly to determine exposure. The problem as posed has a unique solution that may be solved using a variety of approaches. We use a trained artificial neural network (ANN) to derive solutions for hypothetical exposure scenarios. The ANN results show that it is possible to estimate the intensity and timing of past exposures from the material-phase concentration profile in a building material. The overall method is limited by (1) the resolution of techniques for measuring spatial material-phase concentration profiles, (2) how far back in time we seek to determine exposure and (3) the representational power of the ANN solution. For example, we estimate that this technique can estimate exposure to phenol up to 0.5 y in the past from analyses of vinyl flooring.

  19. Deep Seismic Reflection Profiles Reveal The Crust Structures Beneath Xing'an-Mongolian Orogenic Belt and Its Neighboring Area

    NASA Astrophysics Data System (ADS)

    Hou, H.; Gao, R.; Keller, R. G.; Li, Q.; Li, W.; Li, H.; Xiong, X.; Guo, L.

    2012-12-01

    high-order NMO, detailed velocity analysis and maximum energy residual static correction, prestack time migration methods, etc. The migrated stack profiles reveal the deep structural and tectonic relationship between the northern margin of the North China craton and the Xing'an-Mongolia orogenic belt. Many curved reflection phases with strong amplitudes beneath the igneous province suggest magma activity obversed. It is notable that a strong Moho reflection appears to be spatially associated with ancient collision or subduction zones exhibits complicated characters. This scenario may suggest multiple tectonic events that are spatially correlated from topgraphy to deep structure. We think the east-dipping reflections beneath the eastside of Great Xing'An Mountain Range within upper crust related with the Mesozoic detachment event in North China. All the profiles provides a rare opportunity to study signatures of ancient orogenic processes in the context of a continental interior. This research is supported by SinoProbe-02, China NSF (No.40830316, No.41104060), China Geological Survey (No.1212011120975), US NSF PIRE grant (0730154).

  20. Sodium storage in deep paleoweathering profiles beneath the Paleozoic-Triassic unconformity

    NASA Astrophysics Data System (ADS)

    Thiry, M.; Parcerisa, D.; Ricordel-Prognon, C.; Schmitt, J.-M.

    2009-04-01

    A major sodium accumulation has been recognized for long and by numerous authors in the Permo-Triassic salt deposits (Hay et al., 2006). Beside these basinal deposits, important masses of sodium were stored on the continents within deep palaeoweathering profiles in form of albite. Indeed, wide surfaces and huge volumes of granito-gneissic basements of the Hercynian massifs are albitized from North-Africa up to Scandinavia. These albitized rocks have usually been considered as related to tardi-magmatic metasomatic processes (Cathelineau 1986; Petersson and Eliasson 1997). Geometrical arrangement and dating of these alterations point out that these albitizations, or at least a part of them, developed under low temperature subsurface conditions in relation with the Triassic palaeosurface (Ricordel et al., 2007; Parcerisa et al., 2009). Petrology The albitized igneous rocks show a strong alteration with pseudomorphic replacement of the primary plagioclases into albite, replacement of primary biotite by chlorite and minor precipitation of neogenic minerals like albite, chlorite, apatite, haematite, calcite and titanite. Albitized rocks are characterized by their pink coloration due to the presence of minute haematite inclusions in the albite. The development and distribution of the albitization and related alterations above the unaltered basement occurs in three steps that define a vertical profile, up to 100-150 m depth. 1) In the lower part of the profile, albitization occurs within pink-colored patches in the unaltered rock, giving a pink-spotted aspect to the rock. 2) In the middle part of the profile, rocks have an overall pink coloration due to the albitization of the primary Ca-bearing igneous plagioclases. Usually, this facies develops in a pervasive manner, affecting the whole rock, but it may also be restricted to joints, giving a sharp-pink coloration to the fracture wall. 3) Finally, the top of the profile is defined by the same mineral paragenesis as in the

  1. Laser nitriding of iron: Nitrogen profiles and phases

    NASA Astrophysics Data System (ADS)

    Illgner, C.; Schaaf, P.; Lieb, K. P.; Schubert, E.; Queitsch, R.; Bergmann, H.-W.

    1995-07-01

    Armco iron samples were surface nitrided by irradiating them with pulses of an excimer laser in a nitrogen atmosphere. The resulting nitrogen depth profiles measured by Resonant Nuclear Reaction Analysis (RNRA) and the phase formation determined by Conversion Electron Mössbauer Spectroscopy (CEMS) were investigated as functions of energy density and the number of pulses. The nitrogen content of the samples was found to be independent of the number of pulses in a layer of 50 nm from the surface and to increase in depths exceeding 150 nm. The phase composition did not change with the number of pulses. The nitrogen content can be related to an enhanced nitrogen solubility based on high temperatures and high pressures due to the laser-induced plasma above the sample. With increasing pulse energy density, the phase composition changes towards phases with higher nitrogen contents. Nitrogen diffusion seems to be the limiting factor for the nitriding process.

  2. Three-dimensional model of the Rhone deep-sea fan from sea-beam profiles

    SciTech Connect

    Bellaiche, G.; Droz, L.

    1988-08-01

    The authors model has been elaborated from the sea-beam map of the Rhone deep-sea fan established from a network of 328 parallel and overlapping sea-beam profiles 40 to 60 km long, issued from the Deltarho-Profans cruises of the R.V. Jean-Charcot. It represents marine areas ranging from /minus/2,580 to /minus/200 m. The area above /minus/200 m (marine and continental) has been constructed from preexisting data. This model has been built by Szep (Laboratoire de Geodynamique, Villefranche) at a scale of 1/200,000 by superimposing and pasting on a series of cut-out polystyrene sheets. The thickness of these sheets varies from 5 mm (corresponding to 50 m elevation), in the areas shallower than /minus/2,000 m, to 2 mm (20 m elevation) in the deeper areas in order to provide smoother, more precise fan morphology. The resulting vertical exaggeration is 20. The size of the model, in centimeters, is 144 (L) /times/ 78 (W) /times/ 33 (H). It weighs about 15 kg. All the main features displayed by the sea-beam map are striking: meandering deep central channel, abandoned channels, new fan construction linked with channel avulsion, gravity fault scars, damming salt domes. Other features such as deep, small basins at the foot of the canyons are very clear and illustrate the strength of the erosive processes occurring in these areas.

  3. Conjugate margins of Canada and Europe: Results from deep reflection profiling

    NASA Astrophysics Data System (ADS)

    Keen, C.; Peddy, C.; de Voogd, B.; Matthews, D.

    1989-02-01

    Deep seismic reflection profiles have been collected across the conjugate margins of the North Atlantic Ocean. The eastern North Atlantic margin is traversed in the Goban Spur region, and the western North Atlantic margin is crossed in the vicinity of Flemish Cap in the Grand Banks region. These seismic profiles allow us to examine the deep structures and mode of extension in crust that was once contiguous. The Flemish Cap and Goban Spur margins have different structural styles: thick and relatively unfaulted crust is present on the Flemish Cap margin west of the continent-ocean boundary, whereas the Goban Spur margin exhibits a zone of extensional faulting and thinned continental crust. The restored rift zone displays overall symmetry in which a bridge of thin crust (< 15 km thick), about 60 km wide, joins the two crustal blocks of normal continental thickness (˜28 km). The configuration of the Moho, the geometry and distribution of the lower crustal reflections, and the overall symmetry of the rift zone favor pure-shear stretching in the lower lithosphere, although either pure or simple shear could accommodate crustal extension.

  4. Influence of phase delay profile on diffraction efficiency of liquid crystal optical phased array

    NASA Astrophysics Data System (ADS)

    Xu, Lin; Zhang, Jian; Wu, Li Ying

    2009-06-01

    The hardware structure and driving voltage of liquid crystal optical phased array (LCOPA) devices determine the produced phase delay characteristics. The phase delay profile influences directly the device's diffraction efficiency. In this paper, a sawtooth-shaped phase delay model of LCOPA was proposed to analyze quantitatively the influence factors of diffraction efficiency employing Fourier optics theory. Analysis results show that flyback region size is the main factor that affects diffraction efficiency. The influence extent varies with different maximum-phase-delays and grating periods. There exists an optimized curve between maximum-phase-delay and flyback region, and between maximum-phase-delay and grating period, individually. The smaller the grating period is or the larger the flyback region is, the more evident the optimization effect becomes, and the maximum increase ratio is up to 16%. Some feasible experiments were done to test theoretical analysis, and the experimental results agreed with the analysis results.

  5. Deep mantle structure and the postperovskite phase transition.

    PubMed

    Helmberger, D; Lay, T; Ni, S; Gurnis, M

    2005-11-29

    Seismologists have known for many years that the lowermost mantle of the Earth is complex. Models based on observed seismic phases sampling this region include relatively sharp horizontal discontinuities with strong zones of anisotropy, nearly vertical contrasts in structure, and small pockets of ultralow velocity zones (ULVZs). This diversity of structures is beginning to be understood in terms of geodynamics and mineral physics, with dense partial melts causing the ULVZs and a postperovskite solid-solid phase transition producing regional layering, with the possibility of large-scale variations in chemistry. This strong heterogeneity has significant implications on heat transport out of core, the evolution of the magnetic field, and magnetic field polarity reversals. PMID:16217029

  6. Sodium storage in deep paleoweathering profiles beneath the Paleozoic-Triassic unconformity

    NASA Astrophysics Data System (ADS)

    Thiry, M.; Parcerisa, D.; Ricordel-Prognon, C.; Schmitt, J.-M.

    2009-04-01

    A major sodium accumulation has been recognized for long and by numerous authors in the Permo-Triassic salt deposits (Hay et al., 2006). Beside these basinal deposits, important masses of sodium were stored on the continents within deep palaeoweathering profiles in form of albite. Indeed, wide surfaces and huge volumes of granito-gneissic basements of the Hercynian massifs are albitized from North-Africa up to Scandinavia. These albitized rocks have usually been considered as related to tardi-magmatic metasomatic processes (Cathelineau 1986; Petersson and Eliasson 1997). Geometrical arrangement and dating of these alterations point out that these albitizations, or at least a part of them, developed under low temperature subsurface conditions in relation with the Triassic palaeosurface (Ricordel et al., 2007; Parcerisa et al., 2009). Petrology The albitized igneous rocks show a strong alteration with pseudomorphic replacement of the primary plagioclases into albite, replacement of primary biotite by chlorite and minor precipitation of neogenic minerals like albite, chlorite, apatite, haematite, calcite and titanite. Albitized rocks are characterized by their pink coloration due to the presence of minute haematite inclusions in the albite. The development and distribution of the albitization and related alterations above the unaltered basement occurs in three steps that define a vertical profile, up to 100-150 m depth. 1) In the lower part of the profile, albitization occurs within pink-colored patches in the unaltered rock, giving a pink-spotted aspect to the rock. 2) In the middle part of the profile, rocks have an overall pink coloration due to the albitization of the primary Ca-bearing igneous plagioclases. Usually, this facies develops in a pervasive manner, affecting the whole rock, but it may also be restricted to joints, giving a sharp-pink coloration to the fracture wall. 3) Finally, the top of the profile is defined by the same mineral paragenesis as in the

  7. Performance evaluation of digital phase-locked loops for advanced deep space transponders

    NASA Technical Reports Server (NTRS)

    Nguyen, T. M.; Hinedi, S. M.; Yeh, H.-G.; Kyriacou, C.

    1994-01-01

    The performances of the digital phase-locked loops (DPLL's) for the advanced deep-space transponders (ADT's) are investigated. DPLL's considered in this article are derived from the analog phase-locked loop, which is currently employed by the NASA standard deep space transponder, using S-domain to Z-domain mapping techniques. Three mappings are used to develop digital approximations of the standard deep space analog phase-locked loop, namely the bilinear transformation (BT), impulse invariant transformation (IIT), and step invariant transformation (SIT) techniques. The performance in terms of the closed loop phase and magnitude responses, carrier tracking jitter, and response of the loop to the phase offset (the difference between in incoming phase and reference phase) is evaluated for each digital approximation. Theoretical results of the carrier tracking jitter for command-on and command-off cases are then validated by computer simulation. Both theoretical and computer simulation results show that at high sampling frequency, the DPLL's approximated by all three transformations have the same tracking jitter. However, at low sampling frequency, the digital approximation using BT outperforms the others. The minimum sampling frequency for adequate tracking performance is determined for each digital approximation of the analog loop. In addition, computer simulation shows that the DPLL developed by BT provides faster response to the phase offset than IIT and SIT.

  8. Generation of Data-Rate Profiles of Ka-Band Deep-Space Links

    NASA Technical Reports Server (NTRS)

    Shambayati, Shervin

    2006-01-01

    A short report discusses a methodology for designing Ka-band Deep-Space-to- Earth radio-communication links. This methodology is oriented toward minimizing the effects of weather on the Ka-band telecommunication link by maximizing the expected data return subject to minimum link availability and a limited number of data rates. This methodology differs from the current standard practices in which a link is designed according to a margin policy for a given link availability at 10 elevation. In this methodology, one chooses a data-rate profile that will maximize the average data return over a pass while satisfying a minimum-availability requirement for the pass, subject to mission operational limitations expressed in terms of the number of data rates used during the pass. The methodology is implemented in an intelligent search algorithm that first finds the allowable data-rate profiles from the mission constraints, spacecraft-to-Earth distance, spacecraft EIRP (effective isotropic radiated power), and the applicable zenith atmospheric noise temperature distribution, and then selects the best data rate in terms of maximum average data return from the set of allowable data-rate profiles.

  9. POLCRUST - a deep reflection seismic profile across the Trans-European Suture Zone in SE Poland

    NASA Astrophysics Data System (ADS)

    Guterch, A.; Malinowski, M.; Maksym, A.; Probulski, J.; Majdański, M.; Narkiewicz, M.; Czuba, W.; Gaczyński, E.; Grad, M.; Janik, T.; Jankowski, L.; Środa, P.

    2012-04-01

    A 240-km long deep reflection seismic profile (called POLCRUST) has been recently acquired in SE Poland. It is the first regional reflection profile ever acquired in the country. It traverses major tectonic units of SE Poland: East European Craton (EEC) with the Lublin Basin, Paleozoic terranes forming the Trans-European Suture Zone (TESZ) (e.g. the Malopolska Block) with the Carpathian Foredeep and finally the young Alpine orogen, i.e. the Carpathians. The data were acquired with state-of-the art parameters (30 m receiver spacing, 60 m shot spacing) and high CDP fold (175). Most of the sources were Vibroseis (4 trucks) with very high source effort (45 s long sweeps). Correlated record length was 30 s. During the reflection data acquisition we also deployed refraction-type recorders (single channel RefTek-125) every 1.2 km along the line in order to record Vibroseis shots and produce common-receiver gather with extended offset range. This piggy-back experiment resulted in seismic sections with offset up to 25-30 km, which is significantly above the nominal offset range of the reflection spread (10 km). Using the refraction measurements we build a first-break tomography model that was used in further seismic imaging. Reflection seismic data were processed commercially. Some post-stack in-house processing was implemented to enhance the deeper part of the data. Signal penetration limit was estimated to range from 20 s in the NE part (EEC) to ca. 15 s in the SW part (Carpathians). For the interpretation of the deep crustal structure we used data from the nearby refraction/wide-angle reflection profiles from the CELEBRATION 2000 experiment (profiles CEL05 and CEL11). Velocity models were used for in-house post-stack migration and depth-conversion of reflection data. The POLCRUST-01 profile portraits the whole sedimentary cover with an unprecedented resolution. For the first time, the full structural setting of the Lublin Basin can be traced. We imaged the extended crust

  10. Deep seismic reflection profiling of sedimentary basins offshore Brazil: Geological objectives and preliminary results in the Sergipe Basin

    NASA Astrophysics Data System (ADS)

    Mohriak, Webster Ueipass; Lira Rabelo, JoséHenrique; De Matos, Renato Darros; De Barros, Mozart C.

    1995-12-01

    The first deep seismic reflection profiles offshore Brazil were acquired in Campos Basin and processed to 10 s TWT in 1984. Starting in 1989, Petrobrás acquired an extensive data set of deep seismic profiles using special acquisition equipment capable of effectively penetrating through the sedimentary layers and imaging the whole crustal architecture. These deep (18 s TWT) seismic reflection profiles extend across the Atlantic-type marginal basins, from the platform to the deepwater province, presently considered frontier regions for petroleum exploration. This work addresses the geological objectives of a deep seismic profile in the Sergipe Basin and discusses the results obtained by integrating regional seismic, gravity and magnetic data. When combined, these data provide evidence that deep seismic reflectors observed in the Sergipe Basin are related to intracrustal-upper mantle structures rather than sedimentary features. The deep seismic reflection profile in the Sergipe Basin also suggests that, rather than a non-volcanic passive margin, the deepwater extension of this basin is marked by several magmatic structures, including thick wedges of seaward-dipping reflectors and volcanic plugs. These magmatic features are associated with basinforming processes resulting from lithospheric extension during the breakup of Gondwana in the Early Cretaceous and subsequent emplacement of oceanic crust. These results are compared to the crustal scale structures observed in the Campos Basin, in the southeastern margin of Brazil. The interpretation of the deep structure of these basins indicates that final separation between the South American and African plates formed passive margins characterized by different patterns of crustal attenuation underlying the rift blocks.

  11. 30 CFR 203.41 - If I have a qualified deep well or a qualified phase 1 ultra-deep well, what royalty relief would...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false If I have a qualified deep well or a qualified phase 1 ultra-deep well, what royalty relief would my lease earn? 203.41 Section 203.41 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN ROYALTY RATES...

  12. 30 CFR 203.42 - What conditions and limitations apply to royalty relief for deep wells and phase 1 ultra-deep wells?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false What conditions and limitations apply to royalty relief for deep wells and phase 1 ultra-deep wells? 203.42 Section 203.42 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN ROYALTY RATES OCS Oil,...

  13. A simple deep-towed vertical array for high-resolution reflection seismic profiling

    NASA Astrophysics Data System (ADS)

    Herber, R.; Nuppenau, V.; Weigel, W.; Wong, H. K.

    1986-06-01

    A simple, low cost, deep-towed system for high-resolution reflection seismic profiling is described. It consists of a vertical array with two hydrophones having a separation of 2.2 m and rigidly mounted onto streamlined tow bodies. Improvement of the signal-to-noise ratio is attained by simple stacking of the hydrophone outputs after signal conditioning and travel time corrections. The suppression of side echoes and surface reflections is achieved by an analog procedure which in effect improves the directional characteristics of the array. A circuit for automatic gain control is included to enhance weak signals as well as to suppress ringing. Results in Kiel Bay and over the crest of the Jan Mayen Ridge (northern Atlantic) suggest that this simple vertical array may supplement air gun systems better than conventional, surface pinger-type equipment.

  14. Low-fold, ultra-deep MCS Profiling of the Mariana Arc

    NASA Astrophysics Data System (ADS)

    Tidwell, T.; Klemperer, S. L.; Kerr, B. C.; Goodliffe, A. M.

    2002-12-01

    We collected 2600 km of 12- and 15-fold MCS profiles in April 2002, using the R/V Ewing 6-km 240-channel streamer and a tuned 20-airgun array (10,810 cu. in.). The unusually low fold resulted because our primary focus was OBS wide-angle recording (Kerr et al., this session), so airguns were fired at a shot-interval of 90 to 110 s (shot-spacing of 200 m or 250 m) to allow water-column noise to decay between shots. As a positive aspect we recorded 60 s record lengths (the Syntrak-480 recording system could not record longer traces), so potentially imaging structure to over 200-km depth. We acquired 4 arc-parallel profiles: along the back-arc margin of the Mariana arc 30 km west of the main arc; along the volcanic line from 14°30'N (near Rota) to 18°00'N (latitude of Pagan), 30 km east of the arc along the uplifted forearc high; and along the forearc 80 km east of the arc but 140 km west of the trench. We also shot 6 arc-perpendicular lines to link the arc-parallel lines. All profiles were processed through post-stack migration during the cruise, but to only 20 s travel-time. Despite the low fold, the data provide excellent images of shallow basins between the volcanoes that shed light on the volumes and relative timings of eruptions along the arc, and of normal faults particularly along our eastern fore-arc line. Large and rapid topographic variations along the volcanic arc and uplifted forearc cause significant problems of sideswipe (Gunther et al., this session); and strong water-bottom multiples make recognition of deep structure almost impossible on the preliminarily processed data. However, beneath the shallowest, smoothest bathymetry (the guyot extending north of Farallon de Mendenilla in the uplifted forearc high) clear reflections are seen to 6 s (c. 15 km). In principle our low-frequency high-power source should penetrate deep into the mantle, and our streamer was towed at 12-m depth to minimize swell noise and enhance low-frequency signals. Currently we

  15. Anatomy of the southern Cordilleran hingeline, Utah and Nevada, from deep electrical resistivity profiling

    SciTech Connect

    Wannamaker, P.E.; Stodt, J.A.; Johnston, J.M.; Booker, J.R.

    1997-07-01

    To address outstanding questions in Mesozoic-Cenozoic structure and present-day deep physicochemical state in the region of the southern Cordilleran hingeline, a detailed, east-west profile of magnetotelluric (MT) soundings 155 km in length was acquired. From these soundings, a resistivity interpretation was produced using an inversion algorithm based on a structural parameterization. In the upper ten kilometers of the transect, the interpretation shows two segments of low resistivity lying beneath allochthonous rocks of the Late Mesozoic, Sevier thrust sheet. Subsequent industry drilling motivated in part by this surveying confirms the existence and position of the eastern subthrust conductor and, more spectacularly, identifies the presence of yet deeper, autochthonous Mesozoic rocks. A simple structural interpretation is offered where one underthrust segment of low-resistivity sediments was created originally, but this segment was broken later into two major ones during higher-angle Tertiary extension. For the middle and lower crust, the MT data imply a nearly 1-D resistivity structure of remarkable uniformity across the entire transect. Pre-existing structural fabrics have had no measurable influence on localizing regions of high temperature fluids and melting in the lower crust, at least averaged over the scale of tens of kilometers. Given its uniformity over a distance of 155 km or more, the depth to the regional deep conductor does not appear related to the distribution of high-temperature geothermal resources.

  16. Deep seismic reflection profiling of the subduction megathrust across the Sagimi trough and Tokyo bay, Japan

    NASA Astrophysics Data System (ADS)

    Sato, Hiroshi; Iwasaki, Takaya; Abe, Susumu; Saito, Hideo; Kawanaka, Taku; Hirata, Naoshi

    2010-05-01

    Beneath the metropolitan Tokyo, the Philippine Sea plate, in particular the fore arc portion of the Izu-Bonin island arc, has been subducted. Subduction megathrust beneath Tokyo generated M-8 class earthquakes, such as the 1923 Kanto (M7.9) and 1703 Genroku (M8.0) earthquakes. Due to the buyant subduction of the Izu-Bonin arc, the megathrust lies very shallow part of the crust. The Kozu-Matsuda fault, probable spray fault from the megathrust, emerged at the surface. In 2009, we acquired the deep seismic reflection data across the toe of the thrust system to reveal the connectivity of the probable spray fault to the megathrust. Together with the deep seismic section acquired in 2003, we show a 120-km-long deep seismic reflection profile from the front to 30 km in depth and discuss the geometry and characteristics of the thrust system. We performed deep seismic profiling across the Sagami trough for a 70-km-long seismic line in September 2009, using two ships for offshore seismic data acquisition: a gun-ship with a 3020 cu. inch air-gun and a cable-ship with a 2-km-long, streamer cable and a 480 cu. inch air-gun. The seismic signals were recorded at Miura and Izu peninsulas located both ends of the seismic line. At both sides of the onshore line, off-line recorders were deployed along total 20-km-long seismic lines at a 50m interval. Seismic reflection data were acquired by different offset of ships making large-offset gathers. The northeast end of the seismic line connected with the 2003 Tokyo bay seismic line (Sato et al., 2005: Science). The obtained seismic sections portray the detailed geometry of the spray faults, suggesting an emergent thrust with 4 km thick landward dipping strata. It merges to the megathrust at 6-7 sec (TWT). Judging from the geometry of fault-related fold in the trough fill sediments, the tip of the megathrust is located at 3 sec (TWT) beneath the trough axis. According to the co-seismic crustal deformation, the slip of the 1923 Kanto

  17. Deep RNA profiling identified CLOCK and molecular clock genes as pathophysiological signatures in collagen VI myopathy.

    PubMed

    Scotton, Chiara; Bovolenta, Matteo; Schwartz, Elena; Falzarano, Maria Sofia; Martoni, Elena; Passarelli, Chiara; Armaroli, Annarita; Osman, Hana; Rodolico, Carmelo; Messina, Sonia; Pegoraro, Elena; D'Amico, Adele; Bertini, Enrico; Gualandi, Francesca; Neri, Marcella; Selvatici, Rita; Boffi, Patrizia; Maioli, Maria Antonietta; Lochmüller, Hanns; Straub, Volker; Bushby, Katherine; Castrignanò, Tiziana; Pesole, Graziano; Sabatelli, Patrizia; Merlini, Luciano; Braghetta, Paola; Bonaldo, Paolo; Bernardi, Paolo; Foley, Reghan; Cirak, Sebahattin; Zaharieva, Irina; Muntoni, Francesco; Capitanio, Daniele; Gelfi, Cecilia; Kotelnikova, Ekaterina; Yuryev, Anton; Lebowitz, Michael; Zhang, Xiping; Hodge, Brian A; Esser, Karyn A; Ferlini, Alessandra

    2016-04-15

    Collagen VI myopathies are genetic disorders caused by mutations in collagen 6 A1, A2 and A3 genes, ranging from the severe Ullrich congenital muscular dystrophy to the milder Bethlem myopathy, which is recapitulated by collagen-VI-null (Col6a1(-/-)) mice. Abnormalities in mitochondria and autophagic pathway have been proposed as pathogenic causes of collagen VI myopathies, but the link between collagen VI defects and these metabolic circuits remains unknown. To unravel the expression profiling perturbation in muscles with collagen VI myopathies, we performed a deep RNA profiling in both Col6a1(-/-)mice and patients with collagen VI pathology. The interactome map identified common pathways suggesting a previously undetected connection between circadian genes and collagen VI pathology. Intriguingly, Bmal1(-/-)(also known as Arntl) mice, a well-characterized model displaying arrhythmic circadian rhythms, showed profound deregulation of the collagen VI pathway and of autophagy-related genes. The involvement of circadian rhythms in collagen VI myopathies is new and links autophagy and mitochondrial abnormalities. It also opens new avenues for therapies of hereditary myopathies to modulate the molecular clock or potential gene-environment interactions that might modify muscle damage pathogenesis. PMID:26945058

  18. Deep transcriptome profiling of clinical Klebsiella pneumoniae isolates reveals strain and sequence type-specific adaptation.

    PubMed

    Bruchmann, Sebastian; Muthukumarasamy, Uthayakumar; Pohl, Sarah; Preusse, Matthias; Bielecka, Agata; Nicolai, Tanja; Hamann, Isabell; Hillert, Roger; Kola, Axel; Gastmeier, Petra; Eckweiler, Denitsa; Häussler, Susanne

    2015-11-01

    Health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence types, they were more homogenous among isolates of the same sequence type. We furthermore linked the detailed information on differentially expressed genes with the clinically relevant phenotypes of biofilm formation and bacterial virulence. This allowed for the identification of a diminished expression of biofilm-specific genes within the low biofilm producing ST258 isolates as a sequence type-specific trait. PMID:26261087

  19. Deep-cryogenic-treatment-induced phase transformation in the Al-Zn-Mg-Cu alloy

    NASA Astrophysics Data System (ADS)

    Li, Chun-mei; Cheng, Nan-pu; Chen, Zhi-qian; Guo, Ning; Zeng, Su-min

    2015-01-01

    An aluminum alloy (Al-Zn-Mg-Cu) subjected to deep cryogenic treatment (DCT) was systematically investigated. The results show that a DCT-induced phase transformation varies the microstructures and affects the mechanical properties of the Al alloy. Both Guinier-Preston (GP) zones and a metastable η' phase were observed by high-resolution transmission electron microscopy. The phenomenon of the second precipitation of the GP zones in samples subjected to DCT after being aged was observed. The viability of this phase transformation was also demonstrated by first-principles calculations.

  20. Review of recent results from continental deep seismic profiling in Australia

    NASA Astrophysics Data System (ADS)

    Goleby, Bruce R.; Drummond, Barry J.; Korsch, Russell J.; Willcox, J. Barry; O'Brien, Geoffrey W.; Wake-Dyster, Kevin D.

    1994-04-01

    The Australian Geological Survey Organisation regularly collects 450-500 km of onshore deep seismic reflection data and up to 4500 km offshore each year in Australia. These recordings are made in a wide range of tectonic provinces, including, in the last few years, late Palaeozoic-Mesozoic intracontinental and Palaeozoic-Mesozoic-Cenozoic continental margin extensional basins, moderately deformed Palaeozoic transtensional basins and compressional fold belts, and Archaean greenstone terranes. Several of these provinces are major petroleum exploration provinces, whereas others contain significant mineral deposits. The primary purpose of the deep seismic profiling program is to resolve the tectonic history of the Australian continent, and thereby to encourage exploration for hydrocarbons and mineral resources in Australia. On the northwest Australian continental margin, major basin systems including the Bonaparte Basin, formed as a result of complex interactions since the Carboniferous, involving episodes of extension followed by strike-slip movements and inversion, which reactivated both the primary extensional and ancient basement structures. Off southeastern Australia, basins such as the Gippsland Basin formed as part of a linked transtensional system related to movement on a common mid-crustal detachment complex. On continental Australia, the Bowen Basin, in the northeast, was deformed by thrust faults that root in a major E-dipping detachment that flattens in the middle crust. The Cobar Basin, in the southeast, is a case where the seismic data support a detachment model in which the upper plate displacement vector can be calculated by plate reconstructions linking the geometry of the detachment surface with that of the basin. The greenstone terranes within the Eastern Goldfields region of Western Australia show crustal-scale fault systems that are planar and steep dipping, more in keeping with those interpreted in data from other Precambrian provinces rather than

  1. Microbiological Profiles of Deep Terrestrial Sedimentary Rocks Revealed by an Aseptic Drilling Procedure

    NASA Astrophysics Data System (ADS)

    Suzuki, Y.; Suko, T.; Fukuda, A.; Kouduka, M.; Nanba, K.; Sakata, S.; Ito, K.

    2009-12-01

    Unlike the near-surface environments, it is difficult to determine the community structure and biogeochemical functions of microorganisms in the deep subsurface mainly due to accessibility without contamination and disturbance. In an inland fore-arc basin in central Japan, we applied a new drilling procedure using deoxygenated and/or filter-sterilized drilling fluid(s). Although DNA-stained and cultivable cell numbers and the contents of phospholipid fatty acids (PLFA) all indicated the presence of metabolically active microbial populations in sedimentary rocks at a depth range from 200 to 350 m, it was not successful to extract DNA from the drilled core samples. During drilling, drilling fluid used for drilling and coring in the borehole was collected from the borehole bottom and subjected to DNA extraction. Quantitative fluorogenic PCR revealed that bacterial DNA were detected in drilling fluid samples when drilling was performed for siltstone and silty sandstone layers with the limited flow of drilling fluid. Bacterial 16S rRNA gene sequences retrieved from the drilling fluid samples below a depth of 324 m were mostly related to Pseudomonas putida or Flavobacterium succinicans, while those related to other Pseudomonas spp. were predominant at depths of 298 and 299m. PLFA profiles of core samples from a depth range between 250 and 351 m showed the abundance of 16:0, 16:1ω7 and 18:1ω9 fatty acids, which are known as major cellular lipid components of Pseudomonas and Flavobacterium spp. From these results, it was suggested that the members of the genera Pseudomonas and F. succinicans might represent dominant microbial populations that inhabit the deep terrestrial sedimentary rocks in Central Japan. This study was supported by grants from the Nuclear and Industrial Safety Agency (NISA) and Japan Nuclear Energy Safety Organization (JNES).

  2. Deep Seismic Reflection Profiling in the Source Region of the 1923 Kanto Earthquake

    NASA Astrophysics Data System (ADS)

    Sato, H.; Hirata, N.; Iwasaki, T.; Koketsu, K.; Ito, T.; Kasahara, K.; Ito, K.; Kawamura, T.; Ikawa, T.; Onishi, M.; Kawanaka, T.; Abe, S.

    2003-12-01

    The location and geometry of the source fault, and crustal velocity structure, provide the basic information for more precise estimation of strong ground motions with devastative earthquakes. The deep seismic profiling around Metropolitan Tokyo (Kanto area) began from 2002 under the project named `Regional Characterization of the Crust in Metropolitan Areas for Prediction of Strong Ground Motion' as five year's project. Deep seismic profiling was performed along the Sagami (Sagami 2003) and Tokyo Bay (Tokyo Bay 2003), to obtain an image of the source fault of the Kanto earthquake of 1923 (M7.9), upper surface of the Philippine Sea plate, and deeper extension of inland active faults. In Sagami 2003, seismic reflection data were acquired along a 75-km-long seismic line from the flank of the Hakone volcano to Tokyo Bay through the coast of Sagami Bay. The seismic source was four vibroseis trucks and air guns (1500 cu. inch). The seismic signals were recorded by geophones (10 Hz) on land along the coast with 20 33-km-long spread. The seismic data was processed by standard CMP-reflection method. The obtained seismic section portrays the east dipping reflectors beneath Odawara at depth ca. 4 km to Kamakura at depth ca. 13 km for 40-km-distance forming a narrow (< 1 km) concentrated zone of reflectors. The location and geometry of reflectors are almost coincidence with the source fault model (model II) proposed by MatsuOura et al. (1980) for the Kanto earthquake using a inverse method from geodetic data. Thus, it is interpreted that the source fault of the earthquake is in the narrow zone of the concentrated reflectors. The deeper extension of the Kozu-Matsuda Fault, showing the one of the highest slip rates (3 mm/y: vertical component) among active faults in Japan and was assessed high seismic risk, merges to the east dipping reflectors at ca. 6.5 km in depth beneath the Oiso hills. In the Tokyo Bay 2003, seismic reflection data were acquired along a 71-km

  3. Orogenic structure of the Eastern Alps, Europe, from TRANSALP deep seismic reflection profiling

    NASA Astrophysics Data System (ADS)

    Lüschen, Ewald; Lammerer, Bernd; Gebrande, Helmut; Millahn, Karl; Nicolich, Rinaldo; Transalp Working Group

    2004-09-01

    The TRANSALP Group, comprising of partner institutions from Italy, Austria and Germany, acquired data on a 340 km long deep seismic reflection line crossing the Eastern Alps between Munich and Venice. Although the field work was split into four campaigns, between fall 1998 and summer 2001, the project gathered for the first time a continuous profile across the Alps using consistent field acquisition and data processing parameters. These sections span the orogen itself, at its broadest width, as well as the editor Fred Davey and the two adjacent basins. Vibroseis and explosion data, complementary in their depth penetration and resolution characteristics, were obtained along with wide-angle and teleseismic data. The profile shows a bi-vergent asymmetric structure of the crust beneath the Alpine axis which reaches a maximum thickness of 55 km, and 80-100 km long transcrustal ramps, the southward dipping 'Sub-Tauern-Ramp' and the northward-dipping 'Sub-Dolomites-Ramp'. Strongly reflective patterns of these ramps can be traced towards the north to the Inn Valley and towards the south to the Valsugana thrust belt, both of which show enhanced seismicity in the brittle upper crust. The seismic sections do not reveal any direct evidence for the presence of the Periadriatic Fault system, the presumed equivalent to the Insubric Line in the Western Alps. According to our new evolutionary model, the Sub-Tauern-Ramp is linked at depth with remnants of the subducted Penninic Ocean. The 'crocodile'-type model describes an upper/lower crustal decoupling and wedging of both the European and the Adriatic-African continents.

  4. Deep seismic investigation of crustal extensional structures in the Danish Basin along the ESTRID-2 profile

    NASA Astrophysics Data System (ADS)

    Sandrin, A.; Thybo, H.

    2008-05-01

    The crust and uppermost mantle in the Danish Basin are investigated by modelling the P-wave velocity distribution along the north-south trending seismic profile ESTRID-2. Seismic tomography and ray inversion modelling demonstrate a variable depth to the top of the crystalline crust, from ~10 km in the northern part of the profile, to ~2 km depth in the southernmost part. The crystalline crust shows very high P-wave velocity in the central part of the profile, with ~6.7 km s-1 at depths as shallow as 12 km, and ~7.3-7.5 km s-1 in the lowermost crust. These values confirm previous results obtained along the orthogonal ESTRID-1 profile and the Eugeno-S profile 2. This high velocity zone in the middle to lower crust is interpreted as a mafic intrusion, which explains a positive gravity anomaly of ~50 mGal (Silkeborg Gravity High). The total length of the intrusion is at least 80 km in the east-west direction and ~25- 35 km in the north-south direction. The estimated thickness of the intrusion, from its top to the Moho level is ~18-20 km, which gives a total minimum volume of ~40-50 000 km3. The reflectivity properties of the Moho discontinuity are variable along the profile. Below the intrusion, the PmP signal is very weak, due to the small velocity contrast between the lowermost crust (~7.4 km s-1) and uppermost mantle (~7.6-7.7 km s-1). The main Moho reflection has a `reverberative' character to the south of the intrusion. This feature is interpreted by layering at the Moho level, possibly due to magmatic underplating. The occurrence of a large crustal mafic intrusion associated with magmatic underplating may be related to extensional/transtensional tectonism in the Tornquist Fan area in the Late Palaeozoic. The extensional event probably caused the opening of a plumbing system for intrusion of mantle derived magma into the crust. The ascending magma may have been injected at upper-middle crustal levels and, during the late phases of the development, `squeezed

  5. Toxicity profile of choline chloride-based deep eutectic solvents for fungi and Cyprinus carpio fish.

    PubMed

    Juneidi, Ibrahim; Hayyan, Maan; Mohd Ali, Ozair

    2016-04-01

    An investigation on the toxicological assessment of 10 choline chloride (ChCl)-based deep eutectic solvents (DESs) towards four fungi strains and Cyprinus carpio fish was conducted. ChCl was combined with materials from different chemical groups such as alcohols, sugars, acids and others to form DESs. The study was carried out on the individual DES components, their aqueous mixture before DES formation and their formed DESs. The agar disc diffusion method was followed to investigate their toxicity on four fungi strains selected as a model of eukaryotic microorganisms (Phanerochaete chrysosporium, Aspergillus niger, Lentinus tigrinus and Candida cylindracea). Among these DESs, ChCl:ZnCl2 exhibited the highest inhibition zone diameter towards the tested fungi growth in vitro, followed by the acidic group (malonic acid and p-toluenesulfonic acid). Another study was conducted to test the acute toxicity and determine the lethal concentration at 50 % (LC50) of the same DESs on C. carpio fish. The inhibition range and LC50 of DESs were found to be different from their individual components. DESs were found to be less toxic than their mixture or individual components. The LC50 of ChCl:MADES is much higher than that of ChCl:MAMix. Moreover, the DESs acidic group showed a lower inhibition zone on fungi growth. Thus, DESs should be considered as new components with different physicochemical properties and toxicological profiles, and not merely compositions of compounds. PMID:26743645

  6. Metastable mantle phase transformations and deep earthquakes in subducting oceanic lithosphere

    USGS Publications Warehouse

    Kirby, S.H.; Stein, S.; Okal, E.A.; Rubie, David C.

    1996-01-01

    Earth's deepest earthquakes occur as a population in subducting or previously subducted lithosphere at depths ranging from about 325 to 690 km. This depth interval closely brackets the mantle transition zone, characterized by rapid seismic velocity increases resulting from the transformation of upper mantle minerals to higher-pressure phases. Deep earthquakes thus provide the primary direct evidence for subduction of the lithosphere to these depths and allow us to investigate the deep thermal, thermodynamic, and mechanical ferment inside slabs. Numerical simulations of reaction rates show that the olivine ??? spinel transformation should be kinetically hindered in old, cold slabs descending into the transition zone. Thus wedge-shaped zones of metastable peridotite probably persist to depths of more than 600 km. Laboratory deformation experiments on some metastable minerals display a shear instability called transformational faulting. This instability involves sudden failure by localized superplasticity in thin shear zones where the metastable host mineral transforms to a denser, finer-grained phase. Hence in cold slabs, such faulting is expected for the polymorphic reactions in which olivine transforms to the spinel structure and clinoenstatite transforms to ilmenite. It is thus natural to hypothesize that deep earthquakes result from transformational faulting in metastable peridotite wedges within cold slabs. This consideration of the mineralogical states of slabs augments the traditional largely thermal view of slab processes and explains some previously enigmatic slab features. It explains why deep seismicity occurs only in the approximate depth range of the mantle transition zone, where minerals in downgoing slabs should transform to spinel and ilmenite structures. The onset of deep shocks at about 325 km is consistent with the onset of metastability near the equilibrium phase boundary in the slab. Even if a slab penetrates into the lower mantle, earthquakes

  7. The Mechanics of Deep Earthquakes: An Experimental Investigation of Slab Phase Changes

    NASA Astrophysics Data System (ADS)

    Santangeli, J. R.; Dobson, D. P.; Hunt, S. A.; Meredith, P. G.

    2014-12-01

    The mechanics of deep earthquakes have remained a puzzle for researchers since 1928 when they were first accurately identified by Kiyoo Wadati1 in Japan. Deep earthquakes show a split distribution, with peaks centered around ~370-420km and ~520-550km. As these events are limited to subducting slabs, it is accepted that they may be due to phase changes in metastable slab material. Indeed, conditions at ~350km depth are nominally appropriate for the olivine - wadsleyite transition, consistent with the anticrack mechanism previously observed in (Mg,Fe)2SiO42. The additional peak around 520km suggests that there is another siesmogenic phase change; candidates include Ca-garnet -> Ca-perovskite, wadsleyite -> ringwoodite and enstatite -> majorite or ilmenite. Importantly, for large scale seismogenesis to occur candidate phase changes must be susceptible to a runaway mechanism. Typically this involves the release of heat during exothermic reactions, which acts to increase reaction and nucleation rates. It is worth noting that the post-spinel reaction (sp -> pv + fp) marks the cessation of deep earthquakes; possibly as a result of being endothermic. This research aims to identify which of these candidates could be responsible for seismogenesis. We use high-pressure split cylinder multi-anvil experiments with acoustic emission detection. Low-pressure analogue materials have been used to allow greater cell sizes and thus sample volumes to enable accurate location of AE to within the sample. The candidate phase is annealed below its phase boundary, and then taken through the boundary by further compression. Acoustic emissions, if generated, are observed in real time and later processed to ensure they emanate from within the sample volume. Initial results indicate that the pryroxene -> ilmenite transition in MgGeO3 is seismogenic, with several orders of magnitude increase in the energy of AE concurrent with the phase boundary. References:1) Wadati, K. (1928) Shallow and deep

  8. Hydride vapor phase GaN films with reduced density of residual electrons and deep traps

    SciTech Connect

    Polyakov, A. Y.; Smirnov, N. B.; Govorkov, A. V.; Yugova, T. G.; Cox, H.; Helava, H.; Makarov, Yu.; Usikov, A. S.

    2014-05-14

    Electrical properties and deep electron and hole traps spectra are compared for undoped n-GaN films grown by hydride vapor phase epitaxy (HVPE) in the regular process (standard HVPE samples) and in HVPE process optimized for decreasing the concentration of residual donor impurities (improved HVPE samples). It is shown that the residual donor density can be reduced by optimization from ∼10{sup 17} cm{sup −3} to (2–5) × 10{sup 14} cm{sup −3}. The density of deep hole traps and deep electron traps decreases with decreased donor density, so that the concentration of deep hole traps in the improved samples is reduced to ∼5 × 10{sup 13} cm{sup −3} versus 2.9 × 10{sup 16} cm{sup −3} in the standard samples, with a similar decrease in the electron traps concentration.

  9. Multiscale deep drawing analysis of dual-phase steels using grain cluster-based RGC scheme

    NASA Astrophysics Data System (ADS)

    Tjahjanto, D. D.; Eisenlohr, P.; Roters, F.

    2015-06-01

    Multiscale modelling and simulation play an important role in sheet metal forming analysis, since the overall material responses at macroscopic engineering scales, e.g. formability and anisotropy, are strongly influenced by microstructural properties, such as grain size and crystal orientations (texture). In the present report, multiscale analysis on deep drawing of dual-phase steels is performed using an efficient grain cluster-based homogenization scheme. The homogenization scheme, called relaxed grain cluster (RGC), is based on a generalization of the grain cluster concept, where a (representative) volume element consists of p  ×  q  ×  r (hexahedral) grains. In this scheme, variation of the strain or deformation of individual grains is taken into account through the, so-called, interface relaxation, which is formulated within an energy minimization framework. An interfacial penalty term is introduced into the energy minimization framework in order to account for the effects of grain boundaries. The grain cluster-based homogenization scheme has been implemented and incorporated into the advanced material simulation platform DAMASK, which purposes to bridge the macroscale boundary value problems associated with deep drawing analysis to the micromechanical constitutive law, e.g. crystal plasticity model. Standard Lankford anisotropy tests are performed to validate the model parameters prior to the deep drawing analysis. Model predictions for the deep drawing simulations are analyzed and compared to the corresponding experimental data. The result shows that the predictions of the model are in a very good agreement with the experimental measurement.

  10. Moho depth variations beneath China continent from deep seismic sounding profiling

    NASA Astrophysics Data System (ADS)

    Teng, Jiwen; Zhang, Zhongjie; Zhang, Yongqian; Pi, Jiaolong; Deng, Yangfan; Zhang, Xiankang; Wang, Chunyong; Gao, Rui; Liu, Cai

    2013-04-01

    Knowledge of Moho depth and crustal structure are required to study and explore the deep process and coupling response of the formation of mountains, basins, rocks and disasters. In the past half century, the geophysicists in China have completed more than 130 seismic refraction and wide-angle reflection profiles with a total length of 60000km and 11 global geosciences transections (GGT) in China. In this study, we aim to make a systematic research into the Moho depth and crustal structure in China based on the data of velocity models of the crust and upper mantle derived from these more than 130 wide-angle seismic profiles acquired in the last 50 years in China and the surrounding areas. With advanced interpolation technique, we obtain Moho map and average P-wave velocity model, and then make the study on the variations in Moho depth and crustal structure in different tectonics in the East Asia. According to our research results, the resultant Moho depth ranges within 10 and 80 km, and is featured with the deepest Moho discontinuity of about 70-85km beneath Tibetan plateau formed by ongoing continent-continent collision; and relatively constant 30-35 km beneath the eastern North China craton enduring destruction of Lithosphere destruction. Also, we analyzed the average crustal thickness of the consolidated crust beneath the three cratons in China, which is characterized by a gradual thickening from east to west with the values of 29~47 km beneath North-China craton (east), 30~56 km beneath Yangtze craton (south) and 42~59 km beneath Tarim craton (west). In addition, there are three major fold tectonic units in the continental domain and the adjacent oceanic areas, namely the Tethyan-Himalayan zone (south and west), the Paleo-Asian zone (northwest and northeast) and the Circum-Pacific zone (east), which in turn are subdivided into 15 orogenic zones. The Moho depth in these 15 orogenic zones is quite different, too.

  11. Optimized Structures for Low-Profile Phase Change Thermal Spreaders

    NASA Astrophysics Data System (ADS)

    Sharratt, Stephen Andrew

    Thin, low-profile phase change thermal spreaders can provide cooling solutions for some of today's most pressing heat flux dissipation issues. These thermal issues are only expected to increase as future electronic circuitry requirements lead to denser and potentially 3D chip packaging. Phase change based heat spreaders, such as heat pipes or vapor chambers, can provide a practical solution for effectively dissipating large heat fluxes. This thesis reports a comprehensive study of state-of-the-art capillary pumped wick structures using computational modeling, micro wick fabrication, and experimental analysis. Modeling efforts focus on predicting the shape of the liquid meniscus inside a complicated 3D wick structure. It is shown that this liquid shape can drastically affect the wick's thermal resistance. In addition, knowledge of the liquid meniscus shape allows for the computation of key parameters such as permeability and capillary pressure which are necessary for predicting the maximum heat flux. After the model is validated by comparison to experimental results, the wick structure is optimized so as to decrease overall wick thermal resistance and increase the maximum capillary limited heat flux before dryout. The optimized structures are then fabricated out of both silicon and copper using both traditional and novel micro-fabrication techniques. The wicks are made super-hydrophilic using chemical and thermal oxidation schemes. A sintered monolayer of Cu particles is fabricated and analyzed as well. The fabricated wick structures are experimentally tested for their heat transfer performance inside a well controlled copper vacuum chamber. Heat fluxes as high as 170 W/cm2 are realized for Cu wicks with structure heights of 100 μm. The structures optimized for both minimized thermal resistance and high liquid supply ability perform much better than their non-optimized counterparts. The super-hydrophilic oxidation scheme is found to drastically increase the maximum

  12. In Vitro and In Vivo Toxicity Profiling of Ammonium-Based Deep Eutectic Solvents

    PubMed Central

    Hayyan, Maan; Looi, Chung Yeng; Hayyan, Adeeb; Wong, Won Fen; Hashim, Mohd Ali

    2015-01-01

    The cytotoxic potential of ammonium-based deep eutectic solvents (DESs) with four hydrogen bond donors, namely glycerine (Gl), ethylene glycol (EG), triethylene glycol (TEG) and urea (U) were investigated. The toxicity of DESs was examined using In Vitro cell lines and In Vivo animal model. IC50 and selectivity index were determined for the DESs, their individual components and their combinations as aqueous solutions for comparison purposes. The cytotoxicity effect of DESs varied depending on cell lines. The IC50 for the GlDES, EGDES, UDES and TEGDES followed the sequence of TEGDES< GlDES< EGDES< UDES for OKF6, MCF-7, A375, HT29 and H413, respectively. GlDES was selective against MCF-7 and A375, EGDES was selective against MCF-7, PC3, HepG2 and HT29, UDES was selective against MCF-7, PC3, HepG2 and HT29, and TEGDES was selective against MCF-7 and A375. However, acute toxicity studies using ICR mice showed that these DESs were relatively toxic in comparison to their individual components. DES did not cause DNA damage, but it could enhance ROS production and induce apoptosis in treated cancer cells as evidenced by marked LDH release. Furthermore, the examined DESs showed less cytotoxicity compared with ionic liquids. To the best of our knowledge, this is the first time that combined In Vitro and In Vivo toxicity profiles of DESs were being demonstrated, raising the toxicity issue of these neoteric mixtures and their potential applicability to be used for therapeutic purposes. PMID:25679975

  13. In Vitro and In Vivo toxicity profiling of ammonium-based deep eutectic solvents.

    PubMed

    Hayyan, Maan; Looi, Chung Yeng; Hayyan, Adeeb; Wong, Won Fen; Hashim, Mohd Ali

    2015-01-01

    The cytotoxic potential of ammonium-based deep eutectic solvents (DESs) with four hydrogen bond donors, namely glycerine (Gl), ethylene glycol (EG), triethylene glycol (TEG) and urea (U) were investigated. The toxicity of DESs was examined using In Vitro cell lines and In Vivo animal model. IC50 and selectivity index were determined for the DESs, their individual components and their combinations as aqueous solutions for comparison purposes. The cytotoxicity effect of DESs varied depending on cell lines. The IC50 for the GlDES, EGDES, UDES and TEGDES followed the sequence of TEGDES< GlDES< EGDES< UDES for OKF6, MCF-7, A375, HT29 and H413, respectively. GlDES was selective against MCF-7 and A375, EGDES was selective against MCF-7, PC3, HepG2 and HT29, UDES was selective against MCF-7, PC3, HepG2 and HT29, and TEGDES was selective against MCF-7 and A375. However, acute toxicity studies using ICR mice showed that these DESs were relatively toxic in comparison to their individual components. DES did not cause DNA damage, but it could enhance ROS production and induce apoptosis in treated cancer cells as evidenced by marked LDH release. Furthermore, the examined DESs showed less cytotoxicity compared with ionic liquids. To the best of our knowledge, this is the first time that combined In Vitro and In Vivo toxicity profiles of DESs were being demonstrated, raising the toxicity issue of these neoteric mixtures and their potential applicability to be used for therapeutic purposes. PMID:25679975

  14. Constraints on dissipation in the deep interiors of Ganymede and Europa from tidal phase-lags

    NASA Astrophysics Data System (ADS)

    Hussmann, Hauke; Shoji, Daigo; Steinbrügge, Gregor; Stark, Alexander; Sohl, Frank

    2016-08-01

    Jupiter's satellites are subject to strong tidal forces which result in variations of the gravitational potential and deformations of the satellites' surfaces on the diurnal tidal cycle. Such variations are described by the Love numbers k_2 and h_2 for the tide-induced potential variation due to internal mass redistribution and the radial surface displacement, respectively. The phase-lags φ _{k_2} and φ _{h_2} of these complex numbers contain information about the rheological and dissipative states of the satellites. Starting from interior structure models and assuming a Maxwell rheology to compute the tidal deformation, we calculate the phase-lags in application to Ganymede and Europa. For both satellites we assume a decoupling of the outer ice-shell from the deep interior by a liquid subsurface water ocean. We show that, in this case, the phase-lag difference Δ φ = φ _{k_2}- φ _{h_2} can provide information on the rheological and thermal state of the deep interiors if the viscosities of the deeper layers are small. In case of Ganymede, phase-lag differences can reach values of a few degrees for high-pressure ice viscosities {<}10^{14} Pa s and would indicate a highly dissipative state of the deep interior. In this case Δ φ is dominated by dissipation in the high-pressure ice layer rather than dissipation within the ice-I shell. These phase lags would be detectable from spacecraft in orbit around the satellite. For Europa Δ φ could reach values exceeding 20° and phase-lag measurements could help distinguish between (1) a hot dissipative silicate mantle which would in thermal equilibrium correspond to a very thin outer ice-I shell and (2) a cold deep interior implying that dissipation would mainly occur in a thick (several tens of km) outer ice-I shell. These measurements are highly relevant for ESA's Jupiter Icy Moons Explorer (JUICE) and NASA's Europa Multiple Flyby Mission, both targeted for the Jupiter system.

  15. Seven hundred years of peat formation recorded throughout a deep floating mire profile from Central Italy

    NASA Astrophysics Data System (ADS)

    Lobianco, Daniela; D'Orazio, Valeria; Miano, Teodoro; Zaccone, Claudio

    2016-04-01

    3), an ash content ranging from 0.8 and 7.4%, an average gravimetric water content of 26.6±7.7 gwater gdrypeat‑1, and a pH generally increasing with depth (from 4.1 to 7.2). The C content along the profile ranged between 35 and 47% (avg., 41±4%), whereas the N between 0.3 and 1.1% (avg., 0.5±0.1%). Main atomic ratios (C/N, H/C and O/C) and FT-IR spectra seem to confirm what found during the visual inspection of the core, i.e., Sphagnum material so well preserved that it is hard to classify it as "peat". In fact, the 14C age dating suggests that the first 110 cm of Sphagnum material accumulated in ca. 55 yrs, thus resulting in an average growing rate of 2 cm yr‑1. The remaining 300 cm (from 100 to 400 cm of depth), i.e., the submerged part of the island, consist of peat showing completely different botanical composition (reed-fen peat and silty peat rich in reeds) and physical and chemical properties. In particular, both bulk density (avg., 0.09±0.05 g cm‑3) and ash content increase, reaching their maximum at 300-325 cm of depth (0.27 g cm‑3 and 17%, respectively), whereas the average gravimetric water content significantly decreases (17.4±9.0 gwater gdrypeat‑1). The pH ranges from 6.6 and 7.4. Both C and N along this section of the profile show higher average contents (44±3 and 1.3±0.6%, respectively) compared to those recorded in the upper 100 cm layer; furthermore, the decrease with depth of C/N, H/C and O/C atomic ratios, as well as main absorption bands of FT-IR spectra, clearly indicate the occurrence of an organic matter highly humified. The estimated accumulation rate for the bottom 300 cm of the island is 0.5 cm yr‑1. At the best of our knowledge, this work represents the first characterization of a (4 m) deep floating mire profile. At Posta Fibreno, the deep water layer below the base of the island (7 m) and the movement on the water surface probably avoided the transition from floating island to firm land, thus allowing this island to

  16. Seven hundred years of peat formation recorded throughout a deep floating mire profile from Central Italy

    NASA Astrophysics Data System (ADS)

    Lobianco, Daniela; D'Orazio, Valeria; Miano, Teodoro; Zaccone, Claudio

    2016-04-01

    3), an ash content ranging from 0.8 and 7.4%, an average gravimetric water content of 26.6±7.7 gwater gdrypeat‑1, and a pH generally increasing with depth (from 4.1 to 7.2). The C content along the profile ranged between 35 and 47% (avg., 41±4%), whereas the N between 0.3 and 1.1% (avg., 0.5±0.1%). Main atomic ratios (C/N, H/C and O/C) and FT-IR spectra seem to confirm what found during the visual inspection of the core, i.e., Sphagnum material so well preserved that it is hard to classify it as "peat". In fact, the 14C age dating suggests that the first 110 cm of Sphagnum material accumulated in ca. 55 yrs, thus resulting in an average growing rate of 2 cm yr‑1. The remaining 300 cm (from 100 to 400 cm of depth), i.e., the submerged part of the island, consist of peat showing completely different botanical composition (reed-fen peat and silty peat rich in reeds) and physical and chemical properties. In particular, both bulk density (avg., 0.09±0.05 g cm‑3) and ash content increase, reaching their maximum at 300-325 cm of depth (0.27 g cm‑3 and 17%, respectively), whereas the average gravimetric water content significantly decreases (17.4±9.0 gwater gdrypeat‑1). The pH ranges from 6.6 and 7.4. Both C and N along this section of the profile show higher average contents (44±3 and 1.3±0.6%, respectively) compared to those recorded in the upper 100 cm layer; furthermore, the decrease with depth of C/N, H/C and O/C atomic ratios, as well as main absorption bands of FT-IR spectra, clearly indicate the occurrence of an organic matter highly humified. The estimated accumulation rate for the bottom 300 cm of the island is 0.5 cm yr‑1. At the best of our knowledge, this work represents the first characterization of a (4 m) deep floating mire profile. At Posta Fibreno, the deep water layer below the base of the island (7 m) and the movement on the water surface probably avoided the transition from floating island to firm land, thus allowing this island to

  17. Trace elements profiles, notably Hg, from a preliminary study of the Apollo 15 deep-drill core.

    NASA Technical Reports Server (NTRS)

    Jovanovic, S.; Reed, G. W., Jr.

    1972-01-01

    The possible thermal gradient near the surface during a lunation is considered together with the heat flow from the interior, the physical process of Hg migration, the results from core and trench samples from previous missions, and other temperature sensitive phenomena that may help understand the processes. U, Os, and Ru concentrations in the deep drill core samples are of potential interest and are summarized in a table. The Os tends to parallel the Hg profile with depth.

  18. First Autonomous Bio-Optical Profiling Float in the Gulf of Mexico Reveals Dynamic Biogeochemistry in Deep Waters

    PubMed Central

    Green, Rebecca E.; Bower, Amy S.; Lugo-Fernández, Alexis

    2014-01-01

    Profiling floats equipped with bio-optical sensors well complement ship-based and satellite ocean color measurements by providing highly-resolved time-series data on the vertical structure of biogeochemical processes in oceanic waters. This is the first study to employ an autonomous profiling (APEX) float in the Gulf of Mexico for measuring spatiotemporal variability in bio-optics and hydrography. During the 17-month deployment (July 2011 to December 2012), the float mission collected profiles of temperature, salinity, chlorophyll fluorescence, particulate backscattering (bbp), and colored dissolved organic matter (CDOM) fluorescence from the ocean surface to a depth of 1,500 m. Biogeochemical variability was characterized by distinct depth trends and local “hot spots”, including impacts from mesoscale processes associated with each of the water masses sampled, from ambient deep waters over the Florida Plain, into the Loop Current, up the Florida Canyon, and eventually into the Florida Straits. A deep chlorophyll maximum (DCM) occurred between 30 and 120 m, with the DCM depth significantly related to the unique density layer ρ = 1023.6 (R2 = 0.62). Particulate backscattering, bbp, demonstrated multiple peaks throughout the water column, including from phytoplankton, deep scattering layers, and resuspension. The bio-optical relationship developed between bbp and chlorophyll (R2 = 0.49) was compared to a global relationship and could significantly improve regional ocean-color algorithms. Photooxidation and autochthonous production contributed to CDOM distributions in the upper water column, whereas in deep water, CDOM behaved as a semi-conservative tracer of water masses, demonstrating a tight relationship with density (R2 = 0.87). In the wake of the Deepwater Horizon oil spill, this research lends support to the use of autonomous drifting profilers as a powerful tool for consideration in the design of an expanded and integrated observing network

  19. First autonomous bio-optical profiling float in the Gulf of Mexico reveals dynamic biogeochemistry in deep waters.

    PubMed

    Green, Rebecca E; Bower, Amy S; Lugo-Fernández, Alexis

    2014-01-01

    Profiling floats equipped with bio-optical sensors well complement ship-based and satellite ocean color measurements by providing highly-resolved time-series data on the vertical structure of biogeochemical processes in oceanic waters. This is the first study to employ an autonomous profiling (APEX) float in the Gulf of Mexico for measuring spatiotemporal variability in bio-optics and hydrography. During the 17-month deployment (July 2011 to December 2012), the float mission collected profiles of temperature, salinity, chlorophyll fluorescence, particulate backscattering (bbp), and colored dissolved organic matter (CDOM) fluorescence from the ocean surface to a depth of 1,500 m. Biogeochemical variability was characterized by distinct depth trends and local "hot spots", including impacts from mesoscale processes associated with each of the water masses sampled, from ambient deep waters over the Florida Plain, into the Loop Current, up the Florida Canyon, and eventually into the Florida Straits. A deep chlorophyll maximum (DCM) occurred between 30 and 120 m, with the DCM depth significantly related to the unique density layer ρ = 1023.6 (R2 = 0.62). Particulate backscattering, bbp, demonstrated multiple peaks throughout the water column, including from phytoplankton, deep scattering layers, and resuspension. The bio-optical relationship developed between bbp and chlorophyll (R2 = 0.49) was compared to a global relationship and could significantly improve regional ocean-color algorithms. Photooxidation and autochthonous production contributed to CDOM distributions in the upper water column, whereas in deep water, CDOM behaved as a semi-conservative tracer of water masses, demonstrating a tight relationship with density (R2 = 0.87). In the wake of the Deepwater Horizon oil spill, this research lends support to the use of autonomous drifting profilers as a powerful tool for consideration in the design of an expanded and integrated observing network for

  20. High-resolution crustal structure of the Yinchuan basin revealed by deep seismic reflection profiling: implications for deep processes of basin

    NASA Astrophysics Data System (ADS)

    Huang, Xingfu; Feng, Shaoying; Gao, Rui; Li, Wenhui

    2016-04-01

    The Yinchuan basin, located on the western margin of the Ordos block, has the characteristics of an active continental rift. A NW-striking deep seismic reflection profile across the center of Yinchuan basin precisely revealed the fine structure of the crust. The images showed that the crust in the Yinchuan basin was characterized by vertical stratifications along a detachment located at a two-way travel time (TWT) of 8.0 s. The most outstanding feature of this seismic profile was the almost flat Mohorovičić discontinuity (Moho) and a high-reflection zone in the lower crust. This sub-horizontal Moho conflicts with the general assumption of an uplifted Moho under sedimentary basins and continental rifts, and may indicate the action of different processes at depth during the evolution of sedimentary basins or rifts. We present a possible interpretation of these deep processes and the sub-horizontal Moho. The high-reflection zone, which consists of sheets of high-density, mantle-derived materials, may have compensated for crustal thinning in the Yinchuan basin, leading to the formation of a sub-horizontal Moho. These high-density materials may have been emplaced by underplating with mantle-sourced magma.

  1. Development of Demand-Controlled Deep Brain Stimulation Techniques Based on Stochastic Phase Resetting

    NASA Astrophysics Data System (ADS)

    Tass, Peter A.

    2003-05-01

    Stimulation techniques are discussed here which make it possible to effectively desynchronize a synchronized cluster of globally coupled phase oscillators in the presence of noise. To this end composite stimuli are used which consist of a first, stronger stimulus followed by a second, weaker stimulus after a constant time delay. The first stimulus controls the dynamics of the cluster by resetting it, whereas the second stimulus desynchronizes the cluster by hitting it in a vulnerable state. The first, resetting stimulus can be a strong single pulse, a high-frequency pulse train or a low-frequency pulse train. The cluster's resynchronization can effectively be blocked by repeated administration of a composite stimulus. Demand controlled deep brain stimulation with these desynchronizing stimulation techniques is suggested for the therapy of patients suffering from tremor-dominant Parkinson's disease or essential tremor as a milder and more efficient therapy compared to the standard permanent high-frequency deep brain stimulation.

  2. Carbon-bearing iron phases and the carbon isotope composition of the deep Earth

    PubMed Central

    Horita, Juske; Polyakov, Veniamin B.

    2015-01-01

    The carbon budget and dynamics of the Earth’s interior, including the core, are currently very poorly understood. Diamond-bearing, mantle-derived rocks show a very well defined peak at δ13C ≈ −5 ± 3‰ with a very broad distribution to lower values (∼−40‰). The processes that have produced the wide δ13C distributions to the observed low δ13C values in the deep Earth have been extensively debated, but few viable models have been proposed. Here, we present a model for understanding carbon isotope distributions within the deep Earth, involving Fe−C phases (Fe carbides and C dissolved in Fe−Ni metal). Our theoretical calculations show that Fe and Si carbides can be significantly depleted in 13C relative to other C-bearing materials even at mantle temperatures. Thus, the redox freezing and melting cycles of lithosphere via subduction upwelling in the deep Earth that involve the Fe−C phases can readily produce diamond with the observed low δ13C values. The sharp contrast in the δ13C distributions of peridotitic and eclogitic diamonds may reflect differences in their carbon cycles, controlled by the evolution of geodynamical processes around 2.5–3 Ga. Our model also predicts that the core contains C with low δ13C values and that an average δ13C value of the bulk Earth could be much lower than ∼−5‰, consistent with those of chondrites and other planetary body. The heterogeneous and depleted δ13C values of the deep Earth have implications, not only for its accretion−differentiation history but also for carbon isotope biosignatures for early life on the Earth. PMID:25512520

  3. Carbon-bearing iron phases and the carbon isotope composition of the deep Earth.

    PubMed

    Horita, Juske; Polyakov, Veniamin B

    2015-01-01

    The carbon budget and dynamics of the Earth's interior, including the core, are currently very poorly understood. Diamond-bearing, mantle-derived rocks show a very well defined peak at δ(13)C ≈ -5 ± 3‰ with a very broad distribution to lower values (∼-40‰). The processes that have produced the wide δ(13)C distributions to the observed low δ(13)C values in the deep Earth have been extensively debated, but few viable models have been proposed. Here, we present a model for understanding carbon isotope distributions within the deep Earth, involving Fe-C phases (Fe carbides and C dissolved in Fe-Ni metal). Our theoretical calculations show that Fe and Si carbides can be significantly depleted in (13)C relative to other C-bearing materials even at mantle temperatures. Thus, the redox freezing and melting cycles of lithosphere via subduction upwelling in the deep Earth that involve the Fe-C phases can readily produce diamond with the observed low δ(13)C values. The sharp contrast in the δ(13)C distributions of peridotitic and eclogitic diamonds may reflect differences in their carbon cycles, controlled by the evolution of geodynamical processes around 2.5-3 Ga. Our model also predicts that the core contains C with low δ(13)C values and that an average δ(13)C value of the bulk Earth could be much lower than ∼-5‰, consistent with those of chondrites and other planetary body. The heterogeneous and depleted δ(13)C values of the deep Earth have implications, not only for its accretion-differentiation history but also for carbon isotope biosignatures for early life on the Earth. PMID:25512520

  4. 30 CFR 203.41 - If I have a qualified deep well or a qualified phase 1 ultra-deep well, what royalty relief would...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... phase 1 ultra-deep well that is: Then your lease earns an RSV on this volume of gas production: (1) An... production: (1) An original well or a sidetrack with a perforated interval the top of which is from 15,000 to... of water and production must begin on this well before May 3, 2009. If drilling of the second...

  5. 30 CFR 203.42 - What conditions and limitations apply to royalty relief for deep wells and phase 1 ultra-deep wells?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... production from the non-qualified well. (g) You qualify for an RSV under paragraphs (b) or (c) of § 203.41... royalty relief under § 203.41. If . . . Then . . . (a) Your lease has produced gas or oil from a well with...) You determine RSV under § 203.41 for the first qualified deep well or qualified phase 1...

  6. MMIC linear-phase and digital modulators for deep space spacecraft X-band transponder applications

    NASA Technical Reports Server (NTRS)

    Mysoor, Narayan R.; Ali, Fazal

    1991-01-01

    The design concepts, analyses, and development of GaAs monolithic microwave integrated circuit (MMIC) linear-phase and digital modulators for the next generation of space-borne communications systems are summarized. The design approach uses a compact lumped element quadrature hybrid and Metal Semiconductor Field Effect Transistors (MESFET)-varactors to provide low loss and well-controlled phase performance for deep space transponder (DST) applications. The measured results of the MESFET-diode show a capacitance range of 2:1 under reverse bias, and a Q of 38 at 10 GHz. Three cascaded sections of hybrid-coupled reflection phase shifters were modeled and simulations performed to provide an X-band (8415 +/- 50 MHz) DST phase modulator with +/- 2.5 radians of peak phase deviation. The modulator will accommodate downlink signal modulation with composite telemetry and ranging data, with a deviation linearity tolerance of +/- 8 percent and insertion loss of less than 8 +/- 0.5 dB. The MMIC digital modulator is designed to provide greater than 10 Mb/s of bi-phase modulation at X-band.

  7. [Deep eutectic solvent: a new kind of mobile phase modifier for hydrophilic interaction liquid chromatography].

    PubMed

    Tan, Ting; Qiao, Xin; Wan, Yiqun; Qiu, Hongdeng

    2015-09-01

    Deep eutectic solvents (DESs) were used as a new kind of mobile phase modifier in hydrophilic interaction liquid chromatography (HILIC). In our experiment, a SiO2 column (150 mm x 4.6 mm, 3 µm) was selected to separate several nucleobases and nucleosides by using the mixed solution of acetonitrile and DES (choline chloride-ethylene glycol (1:3, mol/mol) ) as mobile phase. Subsequently, the concentrations of DESs in acetonitrile and the column temperature on the effect of separation were investigated. According to the experimental results, better separation of nucleobases and nucleosides was obtained by using acetonitrile and DESs mixed solution as mobile phase than that using traditional water-based solution. For example, a baseline separation between cytosine and cytidine cannot be achieved by HILIC with water-based mobile phase, however, greater improvement was gained by HILIC with modified DES-acetonitrile mobile phase. Meanwhile, the retention times of nucleobases and nucleosides decreased as the proportion of DESs in acetonitrile increased, the most significant decrease of which was with cytidine. Similar retention behavior took place with the effect of column temperature. Decreased retention times of the analytes were observed as column temperature increased. The experimental results indicated that this new method may solve some separation difficulties in traditional water-based HILIC, which also successfully verify the feasibility of DESs as mobile phase modifiers. PMID:26753279

  8. Mesozoic(?) lithosphere-scale buckling of the East European Craton in southern Ukraine: DOBRE-4 deep seismic profile

    NASA Astrophysics Data System (ADS)

    Starostenko, Vitaly; Janik, Tomasz; Lysynchuk, Dmytro; Środa, Piotr; Czuba, Wojciech; Kolomiyets, Katherina; Aleksandrowski, Paweł; Gintov, Oleg; Omelchenko, Victor; Komminaho, Kari; Guterch, Aleksander; Tiira, Timo; Gryn, Dmytro; Legostaeva, Olga; Thybo, Hans; Tolkunov, Anatoliy

    2013-04-01

    In order to study the lithospheric structure in southern Ukraine, a seismic wide-angle reflection/refraction project DOBRE-4 was conducted. The 500 km-long profile starts in the SW from the Alpine/Variscan North Dobrudja fold-thrust belt, being part of the Trans-European Suture Zone. It runs to the NE, mostly along the NW Black Sea coastal plain, towards the center of the Precambrian Ukrainian Shield. The field acquisition in October 2009 included 13 chemical shot points with charge sizes 600-1000 kg every 35-50 km and 230 recording stations, every ~2.5 km. The high data quality allows modelling of the P- and S-wave velocity structure along the profile. Two methods were used for the modelling of the seismic data. At first, ray-tracing trial-and-error modelling was developed using arrivals of major refracted and reflected P- and S-wave phases. Next, the amplitudes of the recorded phases were analysed using finite-difference full waveform method. The resulting velocity model shows fairly homogeneous structure of the middle to lower crust both vertically and laterally. The situation is different in the upper crust, with Vp velocities decreasing upwards from c. 6.35 at 15-20 km to 5.9-5.8 km/s at the top of the crystalline basement and to c. 5.15 to 3.80 km/s in Neoproterozoic and Palaeozoic and to 2.70 to 2.30 km/s in Mesozoic strata. Below the upper crust the Vp smoothly increases downward, from c. 6.5 to 6.7-6.8 km/s near the crustal base, making it difficult to differentiate between the middle and lower crust. No Vp velocities exceeding 6.80 km/s have been recorded even in the lowermost part of the crust, unlike in similar profiles on the East European Craton. There is no clear change in the velocity field when moving laterally from the Precambrian platform into the younger tectonic units to the SW. Therefore, on purely seismic grounds it is not possible to distinguish major tectonic units known from the surface. The Moho is, however, clearly delineated by a

  9. Mesozoic(?) lithosphere-scale buckling of the East European Craton in southern Ukraine: DOBRE-4 deep seismic profile

    NASA Astrophysics Data System (ADS)

    Starostenko, V.; Janik, T.; Lysynchuk, D.; Środa, P.; Czuba, W.; Kolomiyets, K.; Aleksandrowski, P.; Gintov, O.; Omelchenko, V.; Komminaho, K.; Guterch, A.; Tiira, T.; Gryn, D.; Legostaeva, O.; Thybo, H.; Tolkunov, A.

    2013-11-01

    In order to study the lithospheric structure in southern Ukraine, a seismic wide-angle reflection/refraction project DOBRE-4 was conducted. The 500-km-long profile starts in the SW from the Alpine/Variscan North Dobrudja Fold-Thrust Belt, being part of the Trans-European Suture Zone. It runs to the NE, mostly along the NW Black Sea coastal plain, towards the centre of the Precambrian Ukrainian Shield. The field acquisition in October 2009 included 13 chemical shot points with charge sizes 600-1000 kg every 35-50 km and 230 recording stations, every ˜2.5 km. The high data quality allows modelling of the P- and S-wave velocity structure along the profile. Two methods were used for the modelling of the seismic data. At first, ray tracing trial-and-error modelling was developed using arrivals of major refracted and reflected P- and S-wave phases. Next, the amplitudes of the recorded phases were analysed using finite-difference full waveform method. The resulting velocity model shows fairly homogeneous structure of the middle to lower crust both vertically and laterally. The situation is different in the upper crust, with Vp velocities decreasing upwards from ca. 6.35 at 15-20 km to 5.9-5.8 km s-1 at the top of the crystalline basement and to ca. 5.15-3.80 km s-1 in Neoproterozoic and Palaeozoic and to 2.70-2.30 km s-1 in Mesozoic strata. Below the upper crust the Vp smoothly increases downward, from ca. 6.50 to 6.7-6.8 km s-1 near the crustal base, making it difficult to differentiate between the middle and lower crust. No Vp velocities exceeding 6.80 km s-1 have been recorded even in the lowermost part of the crust, unlike in similar profiles on the East European Craton. There is no clear change in the velocity field when moving laterally from the Precambrian platform into the younger tectonic units to the SW. Therefore, on purely seismic grounds it is not possible to distinguish major tectonic units known from the surface. The Moho is, however, clearly delineated by

  10. Lithosphere structure of the west Qinling orogenic belt revealed by deep seismic reflection profile

    NASA Astrophysics Data System (ADS)

    Wang, H.

    2009-12-01

    The west Qinling orogen located in the northeastern margin of the Qinghai-Tibet plateau, is transformation zone between the N-S-trending and E-W-trending tectonics in the Chinese continent. Further study of the fine crust structure of the west Qinling orogen and its relationships with surrounding basins have very important significance for understanding tectonic response of the northeastern margin of the plateau about collision convergence of the Indian block and Asian block and learning formation and evolution of the plateau. In 2009, we reprocessed the data of the Tangke-Hezuo deep seismic reflection profiles collected in 2004 across the west Qinling orogen and the northern Songpan block. The new results show the lithosphere fine structure of the west Qinling orogen. Reflection features indicate that an interface at 6.0-7.0s (TWT) divided the crust into the upper and lower crust, whose structural style and deformation are totally different. Integrating geological data, we deduce that the interface at 6.0-7.0s (depth with 18-21 km) was the basement detachment, which made deformation decoupled of the upper and lower crust. The multi-layered reflections in the upper crust reveal the sedimentary covers of the west Qinling orogen, disclose the thickness of the various structure layer and deformation degree, and provide a basis for the prospective evaluation of a multi-metallic mineral and energy exploration. The north dipping strong reflection characteristics of the lower crust in the west Qinling orogen constituted imbricate structure, such imbricate structural features provide seismology evidence for researching the west Qinling thrusting toward the northern Songpan block, and have great significance for studying formation and evolution of the Songpan-Garze structure. Moho reflections are observed around 17.0-17.2s, characterized by nearly horizontal reflections, which implies the west Qinling orogen underwent an intense extension post orogeny caused the lithosphere

  11. Evaluation of the Effects of Honey on Acute-Phase Deep Burn Wounds

    PubMed Central

    Nakajima, Yukari; Mukai, Kanae; Nasruddin; Komatsu, Emi; Iuchi, Terumi; Kitayama, Yukie; Sugama, Junko; Nakatani, Toshio

    2013-01-01

    This study aimed to clarify the effects of honey on acute-phase deep burn wounds. Two deep burn wounds were created on mice which were divided into four groups: no treatment, silver sulfadiazine, manuka honey, and Japanese acacia honey. Wound sizes were calculated as expanded wound areas and sampled 30 minutes and 1–4 days after wounding for histological observation. The wound sections were subjected to hematoxylin and eosin and immunohistological staining to detect necrotic cells, apoptotic cells, neutrophils, and macrophages. The no treatment group formed a scar. The redness around the wound edges in the silver sulfadiazine group was the most intense. All groups exhibited increased wound areas after wounding. The proportions of necrotic cells and the numbers of neutrophils in the manuka and acacia honey groups were lower than those in the no treatment and silver sulfadiazine groups until day 3; however, there were no significant differences between all groups on day 4. These results show that honey treatment on deep burn wounds cannot prevent wound progression. Moreover, comparing our observations with those of Jackson, there are some differences between humans and animals in this regard, and the zone of hyperemia and its surrounding area fall into necrosis, which contributes to burn wound progression. PMID:24348720

  12. A novel explanation of a low-profile mechanism for unsaturated polyester resins using phase diagrams

    SciTech Connect

    Suspene, L.; Fourquier, D.; Yang, Y.S. )

    1990-04-01

    A novel explanation for the mechanism of low profile additives in unsaturated polyester resins by using a concept of a phase diagram was proposed. According to the ternary phase diagrams for the systems of styrene-unsaturated polyester prepolymer-additives (e.g., polyvinyl acetate, polyurethane), the phenomenon of phase separation was explained. Furthermore, the final morphology of cured resins was correlated to the phase diagram. Microscopic observations showed the morphology was controlled by the phase separation. TEM results also clearly showed that the microvoids were formed in the low profile phase, separated from the resin phase, instead of being in the interfacial region as previously reported. It was concluded that a well controlled phase diagram can lead to a shrinkage-controlled low profile system.

  13. Local coexistence of VO2 phases revealed by deep data analysis

    NASA Astrophysics Data System (ADS)

    Strelcov, Evgheni; Ievlev, Anton; Belianinov, Alex; Tselev, Alexander; Kolmakov, Andrei; Kalinin, Sergei V.

    2016-07-01

    We report a synergistic approach of micro-Raman spectroscopic mapping and deep data analysis to study the distribution of crystallographic phases and ferroelastic domains in a defected Al-doped VO2 microcrystal. Bayesian linear unmixing revealed an uneven distribution of the T phase, which is stabilized by the surface defects and uneven local doping that went undetectable by other classical analysis techniques such as PCA and SIMPLISMA. This work demonstrates the impact of information recovery via statistical analysis and full mapping in spectroscopic studies of vanadium dioxide systems, which is commonly substituted by averaging or single point-probing approaches, both of which suffer from information misinterpretation due to low resolving power.

  14. Local coexistence of VO2 phases revealed by deep data analysis

    PubMed Central

    Strelcov, Evgheni; Ievlev, Anton; Belianinov, Alex; Tselev, Alexander; Kolmakov, Andrei; Kalinin, Sergei V.

    2016-01-01

    We report a synergistic approach of micro-Raman spectroscopic mapping and deep data analysis to study the distribution of crystallographic phases and ferroelastic domains in a defected Al-doped VO2 microcrystal. Bayesian linear unmixing revealed an uneven distribution of the T phase, which is stabilized by the surface defects and uneven local doping that went undetectable by other classical analysis techniques such as PCA and SIMPLISMA. This work demonstrates the impact of information recovery via statistical analysis and full mapping in spectroscopic studies of vanadium dioxide systems, which is commonly substituted by averaging or single point-probing approaches, both of which suffer from information misinterpretation due to low resolving power. PMID:27384473

  15. Local coexistence of VO2 phases revealed by deep data analysis.

    PubMed

    Strelcov, Evgheni; Ievlev, Anton; Belianinov, Alex; Tselev, Alexander; Kolmakov, Andrei; Kalinin, Sergei V

    2016-01-01

    We report a synergistic approach of micro-Raman spectroscopic mapping and deep data analysis to study the distribution of crystallographic phases and ferroelastic domains in a defected Al-doped VO2 microcrystal. Bayesian linear unmixing revealed an uneven distribution of the T phase, which is stabilized by the surface defects and uneven local doping that went undetectable by other classical analysis techniques such as PCA and SIMPLISMA. This work demonstrates the impact of information recovery via statistical analysis and full mapping in spectroscopic studies of vanadium dioxide systems, which is commonly substituted by averaging or single point-probing approaches, both of which suffer from information misinterpretation due to low resolving power. PMID:27384473

  16. Local coexistence of VO2 phases revealed by deep data analysis

    DOE PAGESBeta

    Strelcov, Evgheni; Ievlev, Anton; Tselev, Alexander; Kolmakov, Andrei; Kalinin, Sergei V.

    2016-07-07

    We report a synergistic approach of micro-Raman spectroscopic mapping and deep data analysis to study the distribution of crystallographic phases and ferroelastic domains in a defected Al-doped VO2 microcrystal. Bayesian linear unmixing revealed an uneven distribution of the T phase, which is stabilized by the surface defects and uneven local doping that went undetectable by other classical analysis techniques such as PCA and SIMPLISMA. This work demonstrates the impact of information recovery via statistical analysis and full mapping in spectroscopic studies of vanadium dioxide systems, which is commonly substituted by averaging or single point-probing approaches, both of which suffer frommore » information misinterpretation due to low resolving power.« less

  17. Deep arid system hydrodynamics 2. Application to paleohydrologic reconstruction using vadose zone profiles from the northern Mojave Desert

    USGS Publications Warehouse

    Walvoord, M.A.; Phillips, F.M.; Tyler, S.W.; Hartsough, P.C.

    2002-01-01

    Site-specific numerical modeling of four sites in two arid alluvial basins within the Nevada Test Site employs a conceptual model of deep arid system hydrodynamics that includes vapor transport, the role of xeric vegetation, and long-term surface boundary transients. Surface boundary sequences, spanning 110 kyr, that best reproduce measured chloride concentration and matric potential profiles from four deep (230-460 m) boreholes concur with independent paleohydrologic and paleoecological records from the region. Simulations constrain a pluvial period associated with infiltration of 2-5 mm yr-1 at 14-13 ka and denote a shift linked to the establishment of desert vegetation at 13-9.5 ka. Retrodicted moisture flux histories inferred from modeling results differ significantly from those determined using the conventional chloride mass balance approach that assumes only downward advection. The modeling approach developed here represents a significant advance in the use of deep vadose zone profile data from arid regions to recover detailed paleohydrologic and current hydrologic information.

  18. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments

    PubMed Central

    Ingolia, Nicholas T.; Brar, Gloria A.; Rouskin, Silvia; McGeachy, Anna M.; Weissman, Jonathan S.

    2012-01-01

    Recent studies highlight the importance of translational control in determining protein abundance, underscoring the value of measuring gene expression at the level of translation. We present a protocol for genome-wide, quantitative analysis of in vivo translation by deep sequencing. This ribosome profiling approach maps the exact positions of ribosomes on transcripts by nuclease footprinting. The nuclease-protected mRNA fragments are converted into a DNA library suitable for deep sequencing using a strategy that minimizes bias. The abundance of different footprint fragments in deep sequencing data reports on the amount of translation of a gene. Additionally, footprints reveal the exact regions of the transcriptome that are translated. To better define translated reading frames, we describe an adaptation that reveals the sites of translation initiation by pre-treating cells with harringtonine to immobilize initiating ribosomes. The protocol we describe requires 5–7 days to generate a completed ribosome profiling sequencing library. Sequencing and data analysis requires a further 4 – 5 days. PMID:22836135

  19. Deep geometry structure feature of Haiyuan Fault on the northeastern margin of the Qinghai-Tibet Plateau revealed by deep seismic reflection profiling

    NASA Astrophysics Data System (ADS)

    Wang, H.; Gao, R.; Yin, A.; Kuang, Z.; Xiong, X.; Li, W.

    2012-12-01

    Because of incessant uplifting and pushing role of the active Qinghai-Tibet Plateau uplift and pushed role, Cenozoic tectonic deformation and seismic activity is intense, several large deep fault zones distribute in the northeastern margin of the Tibetan area. The Haiyuan fault is a left strike-slip fault zone with the strongest activities and the largest scale among the arc and active fault zones of the northeastern margin of Qinghai-Tibet Plateau, which is an important tectonic zone boundary faults and also an active faults controling earthquake activity. The CAGS carried out the research of the deep seismic reflection profiling with 300 km long across the northern margin of the West Qinling orogen,Linxia basin,the north Qilian and the southern margin of the Alxa block in 2009, supported by Crust Probe Project of China(Sinoprobe-02) and the Natural Science Foundation of China(No.40830316). The migration section were obtained. According to the section showing the different reflection characteristics, the deep geometry of the Haiyuan fault zone and the fine structure of the crust and upper mantle on both sides of fault. The results discolsed that the Haiyuan fault is not a simple steep or moderate, but its geometric shape changes with depth. The continuous reflection characteristics of the Moho under the Haiyuan fault show that Haiyuan fault is not an ultra-crust fault broke up the Moho directly. The research results provide seismological basis for studying the lithosphere deformation mechanism of the northeastern margin of the Qinghai-Tibet Plateau.

  20. Two-phase convective mixing under a buoyant plume of CO2 in deep saline aquifers

    NASA Astrophysics Data System (ADS)

    Emami-Meybodi, Hamid; Hassanzadeh, Hassan

    2015-02-01

    The storage of carbon dioxide (CO2) in deep saline aquifers has been suggested as a promising method for stabilizing the atmospheric concentration of CO2. An accurate evaluation of the CO2 trapping mechanisms, such as convective mixing, is crucial for estimates of storage capacity and security. We recently investigated the gravitational stability of the diffusive boundary layer underneath a capillary transition zone by performing a linear stability analysis, which provides a quantitative description of the onset of convection for the two-phase, buoyancy-driven flow in the presence of the capillary transition zone (Emami-Meybodi and Hassanzadeh, 2013). In this paper, we further examine the effect of the capillary transition zone on the onset of convection and subsequent convective mixing using direct numerical simulations. We describe key features of the two-phase convective mixing for systems with low Rayleigh numbers (Ra ≤ 1000) and the measurement of several global quantities, such as the total CO2 dissolution, Sherwood number, swelling factor, and interface velocity. We show that the commonly used assumption of a sharp CO2-brine interface with constant CO2 concentration at the top of an aquifer (i.e. single-phase system) may lead to erroneous estimates of not only the onset of convection, but also of the rate and magnitude of CO2 dissolution. The significant effect of the capillary transition zone on the dissolution of CO2 under a buoyant plume in saline aquifers is explained; and, the link between the capillary transition zone and the volume change, due to CO2 dissolution and the interface velocity over the mixing process, is demonstrated. Compared to the single-phase system, a crossflow through the interface of the diffusive boundary layer with the capillary transition zone, as well as the upward advance of the interface motion, may enhance the convective mixing early in the period of natural convection. The decrease in the onset time and stronger mass flux

  1. 30 CFR 203.33 - To which production do I apply the RSV earned by qualified phase 2 and phase 3 ultra-deep wells...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... produces 18 BCF, and the qualified well on lease B produces 37 BCF, then the production volume from and... 30 Mineral Resources 2 2011-07-01 2011-07-01 false To which production do I apply the RSV earned... To which production do I apply the RSV earned by qualified phase 2 and phase 3 ultra-deep wells on...

  2. 30 CFR 203.31 - If I have a qualified phase 2 or qualified phase 3 ultra-deep well, what royalty relief would...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false If I have a qualified phase 2 or qualified phase 3 ultra-deep well, what royalty relief would that well earn for my lease? 203.31 Section 203.31 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN...

  3. 30 CFR 203.34 - To which production may an RSV earned by qualified phase 2 and phase 3 ultra-deep wells on my...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false To which production may an RSV earned by qualified phase 2 and phase 3 ultra-deep wells on my lease not be applied? 203.34 Section 203.34 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN ROYALTY...

  4. Digital image profilers for detecting faint sources which have bright companions, phase 2

    NASA Technical Reports Server (NTRS)

    Morris, Elena; Flint, Graham

    1991-01-01

    A breadboard image profiling system developed for the first phase of this project demonstrated the potential for detecting extremely faint optical sources in the presence of light companions. Experimental data derived from laboratory testing of the device supports the theory that image profilers of this type may approach the theoretical limit imposed by photon statistics. The objective of Phase 2 of this program is the development of a ground-based multichannel image profiling system capable of detecting faint stellar objects slightly displaced from brighter stars. We have finalized the multichannel image profiling system and attempted three field tests.

  5. The pyrolytic profile of lyophilized and deep-frozen compact part of the human bone.

    PubMed

    Lodowska, Jolanta; Wolny, Daniel; Kurkiewicz, Sławomir; Węglarz, Ludmiła

    2012-01-01

    Background. Bone grafts are used in the treatment of nonunion of fractures, bone tumors and in arthroplasty. Tissues preserved by lyophilization or deep freezing are used as implants nowadays. Lyophilized grafts are utilized in the therapy of birth defects and bone benign tumors, while deep-frozen ones are applied in orthopedics. The aim of the study was to compare the pyrolytic pattern, as an indirect means of the analysis of organic composition of deep-frozen and lyophilized compact part of the human bone. Methods. Samples of preserved bone tissue were subjected to thermolysis and tetrahydroammonium-hydroxide- (TMAH-) associated thermochemolysis coupled with gas chromatography and mass spectrometry (Py-GC/MS). Results. Derivatives of benzene, pyridine, pyrrole, phenol, sulfur compounds, nitriles, saturated and unsaturated aliphatic hydrocarbons, and fatty acids (C12-C20) were identified in the pyrolytic pattern. The pyrolyzates were the most abundant in derivatives of pyrrole and nitriles originated from proteins. The predominant product in pyrolytic pattern of the investigated bone was pyrrolo[1,2-α]piperazine-3,6-dione derived from collagen. The content of this compound significantly differentiated the lyophilized graft from the deep-frozen one. Oleic and palmitic acid were predominant among fatty acids of the investigated samples. The deep-frozen implants were characterized by higher percentage of long-chain fatty acids than lyophilized grafts. PMID:22619606

  6. The Pyrolytic Profile of Lyophilized and Deep-Frozen Compact Part of the Human Bone

    PubMed Central

    Lodowska, Jolanta; Wolny, Daniel; Kurkiewicz, Sławomir; Węglarz, Ludmiła

    2012-01-01

    Background. Bone grafts are used in the treatment of nonunion of fractures, bone tumors and in arthroplasty. Tissues preserved by lyophilization or deep freezing are used as implants nowadays. Lyophilized grafts are utilized in the therapy of birth defects and bone benign tumors, while deep-frozen ones are applied in orthopedics. The aim of the study was to compare the pyrolytic pattern, as an indirect means of the analysis of organic composition of deep-frozen and lyophilized compact part of the human bone. Methods. Samples of preserved bone tissue were subjected to thermolysis and tetrahydroammonium-hydroxide- (TMAH-) associated thermochemolysis coupled with gas chromatography and mass spectrometry (Py-GC/MS). Results. Derivatives of benzene, pyridine, pyrrole, phenol, sulfur compounds, nitriles, saturated and unsaturated aliphatic hydrocarbons, and fatty acids (C12–C20) were identified in the pyrolytic pattern. The pyrolyzates were the most abundant in derivatives of pyrrole and nitriles originated from proteins. The predominant product in pyrolytic pattern of the investigated bone was pyrrolo[1,2-α]piperazine-3,6-dione derived from collagen. The content of this compound significantly differentiated the lyophilized graft from the deep-frozen one. Oleic and palmitic acid were predominant among fatty acids of the investigated samples. The deep-frozen implants were characterized by higher percentage of long-chain fatty acids than lyophilized grafts. PMID:22619606

  7. EXPERIMENTAL EVALUATION OF CHEMICAL SEQUESTRATION OF CARBON DIOXIDE IN DEEP AQUIFER MEDIA - PHASE II

    SciTech Connect

    Neeraj Gupta; Bruce Sass; Jennifer Ickes

    2000-11-28

    In 1998 Battelle was selected by the U.S. Department of Energy's (DOE) National Energy Technology Laboratory (NETL) under a Novel Concepts project grant to continue Phase II research on the feasibility of carbon dioxide (CO{sub 2}) sequestration in deep saline formations. The focus of this investigation is to conduct detailed laboratory experiments to examine factors that may affect chemical sequestration of CO{sub 2} in deep saline formations. Reactions between sandstone and other geologic media from potential host reservoirs, brine solutions, and CO{sub 2} are being investigated under high-pressure conditions. Some experiments also include sulfur dioxide (SO{sub 2}) gases to evaluate the potential for co-injection of CO{sub 2} and SO{sub 2} related gases in the deep formations. In addition, an assessment of engineering and economic aspects is being conducted. This current Technical Progress Report describes the status of the project as of September 2000. The major activities undertaken during the quarter included several experiments conducted to investigate the effects of pressure, temperature, time, and brine composition on rock samples from potential host reservoirs. Samples (both powder and slab) were taken from the Mt. Simon Sandstone, a potential CO{sub 2} host formation in the Ohio, the Eau Claire Shale, and Rome Dolomite samples that form the caprock for Mt. Simon Sandstone. Also, a sample with high calcium plagioclase content from Frio Formation in Texas was used. In addition, mineral samples for relatively pure Anorthite and glauconite were experimented on with and without the presence of additional clay minerals such as kaolinite and montmorillonite. The experiments were run for one to two months at pressures similar to deep reservoirs and temperatures set at 50 C or 150 C. Several enhancements were made to the experimental equipment to allow for mixing of reactants and to improve sample collection methods. The resulting fluids (gases and liquids) as

  8. Low-flow characteristics and profiles for the Deep River in the Cape Fear River basin, North Carolina

    USGS Publications Warehouse

    Weaver, J.C.

    1997-01-01

    An understanding of the magnitude and frequency of low-flow discharges is an important part of protecting surface-water resources and planning for municipal and industrial economic expansion. Low-flow characteristics are summarized for 7 continuous-record gaging stations and 23 partial-record measuring sites in the Deep River Basin in North Carolina. Records of discharge collected through the 1995 water year are used. Flow characteristics included in the summary are (1) average annual unit flow; (2) 7Q10 low-flow discharge, the minimum average discharge for a 7-consecutive-day period occurring, on average, once in 10 years; (3) 30Q2 low-flow discharge; (4) W7Q10 low-flow discharge, similar to 7Q10 discharge except only flow during the months of November through March is considered; and (5) 7Q2 low-flow discharge. The potential for sustained base flows in the upper half of the basin is higher than that for streams in the lower half of the basin. Much of the decrease in base-flow potential in the lower part of the basin is attributed to the underlying rock types of the Triassic basin. Soils in the lower part of the basin consist of clays which limit the infiltration of water into surficial aquifers for later release to streams during drought conditions. Correspondingly, many streams in the lower part of the basin have minimal (defined as less than 0.1 cubic foot per second) or zero 7Q10 discharges. Drainage area and low-flow discharge profiles are presented for the Deep River. The drainage-area profile shows downstream increases in basin size. At the mouth, the drainage area for the Deep River is 1,441 square miles. Low-flow discharge profiles for the Deep River include 7Q10, 30Q2, W7Q10, and 7Q2 discharges in a continuous profile with contributions from major tributaries included.

  9. Elasticity and rheology of phase D and implications for seismic anisotropy in deep subducted slabs

    NASA Astrophysics Data System (ADS)

    Rosa, A. D.; Sanchez-Valle, C.; Ghosh, S.; Merkel, S.

    2011-12-01

    Dense hydrous magnesium silicate (DHMS) Phase D, MgSi2H2O6 (10-18 wt% H2O), may be the most important water carrier along cold slab geotherms and thus may play a critical role in the recycling of water into the deep mantle. Recent petrological studies have shown that Fe and Al substitutions extends the stability field of phase D toward higher slab temperatures [Ghosh, unpublished], and that phase D can account for more than 40 vol% in very hydrous peridotites between 700 and 1300 km depth [Iwamori 2004]. The stability and large volume fractions of phase D in subducted slabs, together with the highly anisotropic behavior under compression [Hushur et al. 2011] and ability to align in non-hydrostatic stress fields due to its layered structure [Rosa et al. 2010] suggest that phase D may strongly influence the seismic properties of down going slabs [Chen and Brudzinki, 2003; Laurence and Wysession, 2006]. In this contribution we combine elasticity measurements and plastic deformation experiments to evaluate the contribution of phase D to the seismic anisotropy and velocity anomalies of hydrous subducted slabs at lower mantle pressures. The elastic properties were determined by Brillouin scattering spectroscopy on Mg-bearing and Al-Fe-bearing Phase D single-crystals, synthesized at 24 GPa and 1200 °C in a multianvil apparatus at ETH Zurich. The results resolve discrepancies between previous experimental and computational studies [Liu et al 2004; Mainprice at al. 2007] and assess the effect of compositional variations on the seismic velocities of phase D. The end-member Mg-Phase D displays higher acoustic velocities than the Al-Fe-bearing Phase D. Synchrotron X-ray radial diffraction experiments were also performed to investigate the rheological properties (texturing and preferred orientations) of Mg-bearing, Fe-bearing and Al-Fe-bearing samples at pressures up to 45 GPa. Experiments were conducted in panoramic diamond anvil cells at the ESRF ID09 beamline. The uniaxial

  10. 30 CFR 203.40 - Which leases are eligible for royalty relief as a result of drilling a deep well or a phase 1...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... a result of drilling a deep well or a phase 1 ultra-deep well? 203.40 Section 203.40 Mineral... eligible for royalty relief as a result of drilling a deep well or a phase 1 ultra-deep well? Your lease... lease has not produced gas or oil from a well with a perforated interval the top of which is 18,000...

  11. 30 CFR 203.40 - Which leases are eligible for royalty relief as a result of drilling a deep well or a phase 1...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... a result of drilling a deep well or a phase 1 ultra-deep well? 203.40 Section 203.40 Mineral... a result of drilling a deep well or a phase 1 ultra-deep well? Your lease may receive an RSV under... a well with a perforated interval the top of which is 18,000 feet TVD SS or deeper that...

  12. 30 CFR 203.40 - Which leases are eligible for royalty relief as a result of drilling a deep well or a phase 1...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... a result of drilling a deep well or a phase 1 ultra-deep well? 203.40 Section 203.40 Mineral... leases are eligible for royalty relief as a result of drilling a deep well or a phase 1 ultra-deep well.... (b) The lease has not produced gas or oil from a well with a perforated interval the top of which...

  13. 30 CFR 203.40 - Which leases are eligible for royalty relief as a result of drilling a deep well or a phase 1...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... a result of drilling a deep well or a phase 1 ultra-deep well? 203.40 Section 203.40 Mineral... eligible for royalty relief as a result of drilling a deep well or a phase 1 ultra-deep well? Your lease... lease has not produced gas or oil from a well with a perforated interval the top of which is 18,000...

  14. An Evaluation of the Carbon Sequestration Potential of the Cambro-Ordovician Strata of the Illinois and Michigan Basins. Part 1. Evaluation of Phase 2 CO2 Injection Testing in the Deep Saline Gunter Sandstone Reservoir (Cambro-Ordovician Knox Group), Marvin Blan No. 1 Hancock County, Kentucky Part 2. Time-lapse Three-Dimensional Vertical Seismic Profile (3D-VSP) of Sequestration Target Interval with Injected Fluids

    SciTech Connect

    Bowersox, Richard; Hickman, John; Leetaru, Hannes

    2012-12-20

    Part 1 of this report focuses on results of the western Kentucky carbon storage test, and provides a basis for evaluating injection and storage of supercritical CO2 in Cambro-Ordovician carbonate reservoirs throughout the U.S. Midcontinent. This test demonstrated that the Cambro- Ordovician Knox Group, including the Beekmantown Dolomite, Gunter Sandstone, and Copper Ridge Dolomite in stratigraphic succession from shallowest to deepest, had reservoir properties suitable for supercritical CO2 storage in a deep saline reservoir hosted in carbonate rocks, and that strata with properties sufficient for long-term confinement of supercritical CO2 were present in the deep subsurface. Injection testing with brine and CO2 was completed in two phases. The first phase, a joint project by the Kentucky Geological Survey and the Western Kentucky Carbon Storage Foundation, drilled the Marvin Blan No. 1 carbon storage research well and tested the entire Knox Group section in the open borehole – including the Beekmantown Dolomite, Gunter Sandstone, and Copper Ridge Dolomite – at 1152–2255 m, below casing cemented at 1116 m. During Phase 1 injection testing, most of the 297 tonnes of supercritical CO2 was displaced into porous and permeable sections of the lowermost Beekmantown below 1463 m and Gunter. The wellbore was then temporarily abandoned with a retrievable bridge plug in casing at 1105 m and two downhole pressure-temperature monitoring gauges below the bridge plug pending subsequent testing. Pressure and temperature data were recorded every minute for slightly more than a year, providing a unique record of subsurface reservoir conditions in the Knox. In contrast, Phase 2 testing, this study, tested a mechanically-isolated dolomitic-sandstone interval in the Gunter.

  15. Proterozoic basin in the southern Midcontinent of the United States revealed by COCORP deep seismic reflection profiling

    NASA Astrophysics Data System (ADS)

    Brewer, J. A.; Brown, L. D.; Steiner, D.; Oliver, J. E.; Kaufman, S.; Denison, R. E.

    1981-12-01

    COCORP deep crustal seismic profiles in southwestern Oklahoma show strong, persistent, continuous, and undeformed layering in the basement over an area probably very much greater than 2,500 km2. Such layering is very unusual, judging by COCORP experience with basement rocks elsewhere in the United States. The data can be interpreted as representing a Proterozoic basin filled with clastic sedimentary and felsic volcanic rocks 7 to 10 km thick, whose base lies 10 to 13 km deep. These rocks are believed, on the basis of sparse evidence from regional geology, to have been deposited or extruded about 1,200 to 1,400 m.y. ago, and some of them may now be metamorphosed. This basin lies on the south side of the Wichita Mountains, under the Paleozoic Hardeman Basin, and is similar in depth to the Paleozoic Anadarko Basin north of the mountains. The deep basement layering is truncated on the south side of the Wichita Mountains, probably by Precambrian faults in conjunction with granitic intrusions. Pennsylvanian compression probably reactivated these Precambrian trends. Extensive Precambrian basin deposits in this area were unexpected, on the basis of evidence from sparse well control, and reports of other layered basement reflections elsewhere in the southern Midcontinent suggest that Precambrian basins may be an important feature of this region. Simple models for the evolution of southwestern Oklahoma as an aulacogen must be reformulated in the light of these new data.

  16. Origin of deep crystal reflections: seismic profiling across high-grade metamorphic terranes in Canada

    USGS Publications Warehouse

    Green, A.; Milkereit, B.; Percival, J.; Davidson, A.; Parrish, R.; Cook, F.; Geis, W.; Cannon, W.; Hutchinson, D.; West, G.; Clowes, R.

    1990-01-01

    In an attempt to better understand the origin of deep crustal reflections LITHOPROBE has sponsored or co-sponsored Seismic reflection surveys across tracts of high-grade metamorphic rock in the Archean Superior craton, the Proterozoic Grenville orogen and the Phanerozoic Cordilleran orogen. Common to these three diverse terranes are near-surface zones of prominent Seismic reflectivity that are typically associated with velocity discontinuities at highly strained contacts between gneissic rocks of varying lithology. At some locations the reflective layering resulted from transposition and rearrangement of previously layered rocks (stratified assemblages, sills, etc.), whereas in other regions it was generated by extreme attenuation, stretching and ductile flow of weakly layered or irregularly organized rocks. It seems likely that compositionally layered gneissic rock is a common source of reflections in the deep crust, with reflections originating at lithological boundaries and zones of mylonite. ?? 1990.

  17. Deep structure of the Algerian margin offshore Great Kabylie: Preliminary results of an offshore-onshore seismic profile (SPIRAL campaign)

    NASA Astrophysics Data System (ADS)

    Chafik, Aidi; Abd el Karim, Yelles; Marie-Odile, Beslier; Frauke, Klingelhoefer; Philippe, Schnurle; Rabah, Bracene; Hamou, Djellit; Audrey, Galve; Laure, Schenini; Françoise, Sage; Abdallah, Bounif Mohand ou; Philippe, Charvis

    2013-04-01

    In October-November 2009 the Algerian-French SPIRAL research program (Sismique Profonde et Investigation Régionale du Nord de l'ALgérie) was conducted onboard the R/V Atalante in order to understand the deep structure and tectonic history of the Algerian Margin using multichannel and wide-angle seismic data. An extensive dataset was acquired along five regional transects off Algeria, from Arzew Bay to the west, to Annaba to the east. The profiles range from 80 to 180 km long and around 40 ocean-bottom seismometers were deployed on each profile. All profiles were extended on land up to 125 km by land-stations to better constrain the structure of the margin and the nature of the ocean-continent transition zone. We present the preliminary results from modeling of deep and superficial structures in the central Algerian margin, more precisely in the region of the Great Kabylie where a N-S transect of combined wide-angle data using a set of 40 OBS (ocean bottom seismometer) and 24 on-land seismological stations and reflection seismic data was acquired. The profile with a total length of about 260 km (140 km offshore and approximately 124 km onshore), crosses from the north to south the Algeria-Provence Basin, the central Algerian Margin and onshore the geological unit of the Great Kabylie that represents the Kabylides block and the transitional zone between the internal zone (Kabylides) and the external zone in the central Algeria. The network (OBS and seismological stations), recorded 1031 low frequency air gun shots in order to ensure good penetration in the crust. Travel time tomography of first arrivals time of OBS data has yielded a preliminary model of P wave velocities along the profile. In the oceanic domain, a relatively thin crust of about 5 km thickness was imaged overlying a mantle characterized by seismic velocities of about 8 km/s, and covered by a thin sedimentary layer of about 2 km thickness. For the study of the sedimentary cover near the margin

  18. Electron bunch profile reconstruction based on phase-constrained iterative algorithm

    NASA Astrophysics Data System (ADS)

    Bakkali Taheri, F.; Konoplev, I. V.; Doucas, G.; Baddoo, P.; Bartolini, R.; Cowley, J.; Hooker, S. M.

    2016-03-01

    The phase retrieval problem occurs in a number of areas in physics and is the subject of continuing investigation. The one-dimensional case, e.g., the reconstruction of the temporal profile of a charged particle bunch, is particularly challenging and important for particle accelerators. Accurate knowledge of the longitudinal (time) profile of the bunch is important in the context of linear colliders, wakefield accelerators and for the next generation of light sources, including x-ray SASE FELs. Frequently applied methods, e.g., minimal phase retrieval or other iterative algorithms, are reliable if the Blaschke phase contribution is negligible. This, however, is neither known a priori nor can it be assumed to apply to an arbitrary bunch profile. We present a novel approach which gives reproducible, most-probable and stable reconstructions for bunch profiles (both artificial and experimental) that would otherwise remain unresolved by the existing techniques.

  19. Synthesis of fully continuous phase screens for tailoring the focal plane irradiance profiles

    SciTech Connect

    Dixit, S.; Feit, M.

    1996-04-26

    We present an iterative procedure for constructing fully continuous phase screens for tailoring the focal plane intensity distributions. This algorithm alleviates the stagnations experienced in the application of the Gerchberg-Saxton algorithm with a random initial phase screen and leads to efficient distribution of the incident energy into the desired focal plane profile.

  20. Shearing instabilities accompanying high-pressure phase transformations and the mechanics of deep earthquakes

    PubMed Central

    Green, Harry W.

    2007-01-01

    Deep earthquakes have been a paradox since their discovery in the 1920s. The combined increase of pressure and temperature with depth precludes brittle failure or frictional sliding beyond a few tens of kilometers, yet earthquakes occur continually in subduction zones to ≈700 km. The expected healing effects of pressure and temperature and growing amounts of seismic and experimental data suggest that earthquakes at depth probably represent self-organized failure analogous to, but different from, brittle failure. The only high-pressure shearing instabilities identified by experiment require generation in situ of a small fraction of very weak material differing significantly in density from the parent material. This “fluid” spontaneously forms mode I microcracks or microanticracks that self-organize via the elastic strain fields at their tips, leading to shear failure. Growing evidence suggests that the great majority of subduction zone earthquakes shallower than 400 km are initiated by breakdown of hydrous phases and that deeper ones probably initiate as a shearing instability associated with breakdown of metastable olivine to its higher-pressure polymorphs. In either case, fault propagation could be enhanced by shear heating, just as is sometimes the case with frictional sliding in the crust. Extensive seismological interrogation of the region of the Tonga subduction zone in the southwest Pacific Ocean provides evidence suggesting significant metastable olivine, with implication for its presence in other regions of deep seismicity. If metastable olivine is confirmed, either current thermal models of subducting slabs are too warm or published kinetics of olivine breakdown reactions are too fast. PMID:17468397

  1. Measurement of surface profile in vibrating environment with instantaneous phase shifting interferometry

    NASA Astrophysics Data System (ADS)

    Sivakumar, N. R.; Tan, B.; Venkatakrishnan, K.

    2006-01-01

    In-process measurement has been the requirement of the precision industries, but due to vibrations while manufacturing, in-process measurement has been difficult to achieve. There is little work on in-process measurement using phase shifting interferometry, as phase shifting is extremely sensitive to vibrations. In this work, the advantage of the developed non-mechanical and instantaneous phase shifting interferometry is felt while measuring surface profile of large flat surfaces under vibrating conditions which can be extended for in-process measurement of surface profile. A near common path optical configuration is achieved and the effect of the environment is reduced. Moreover, the measurement of phase is instantaneous which increases the versatility of this technique for measuring vibrating objects. Profile measurements were carried out on a smooth mirror surface excited with vibrations of different frequencies and the technique was found to be immune to vibrations of up to 1000 Hz.

  2. 30 CFR 203.33 - To which production do I apply the RSV earned by qualified phase 2 and phase 3 ultra-deep wells...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false To which production do I apply the RSV earned by qualified phase 2 and phase 3 ultra-deep wells on my lease or in my unit? 203.33 Section 203.33 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN...

  3. 30 CFR 203.33 - To which production do I apply the RSV earned by qualified phase 2 and phase 3 ultra-deep wells...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... phase 3 ultra-deep well that earns your lease the RSV begins production (other than test production). (2... well on lease B produces 37 BCF, then the production volume from and allocated to lease A to which the... 30 Mineral Resources 2 2010-07-01 2010-07-01 false To which production do I apply the RSV...

  4. MiRNA Expression Profile for the Human Gastric Antrum Region Using Ultra-Deep Sequencing

    PubMed Central

    Hamoy, Igor G.; Darnet, Sylvain; Burbano, Rommel; Khayat, André; Gonçalves, André Nicolau; Alencar, Dayse O.; Cruz, Aline; Magalhães, Leandro; Araújo Jr., Wilson; Silva, Artur; Santos, Sidney; Demachki, Samia; Assumpção, Paulo; Ribeiro-dos-Santos, Ândrea

    2014-01-01

    Background MicroRNAs are small non-coding nucleotide sequences that regulate gene expression. These structures are fundamental to several biological processes, including cell proliferation, development, differentiation and apoptosis. Identifying the expression profile of microRNAs in healthy human gastric antrum mucosa may help elucidate the miRNA regulatory mechanisms of the human stomach. Methodology/Principal Findings A small RNA library of stomach antrum tissue was sequenced using high-throughput SOLiD sequencing technology. The total read count for the gastric mucosa antrum region was greater than 618,000. After filtering and aligning using with MirBase, 148 mature miRNAs were identified in the gastric antrum tissue, totaling 3,181 quality reads; 63.5% (2,021) of the reads were concentrated in the eight most highly expressed miRNAs (hsa-mir-145, hsa-mir-29a, hsa-mir-29c, hsa-mir-21, hsa-mir-451a, hsa-mir-192, hsa-mir-191 and hsa-mir-148a). RT-PCR validated the expression profiles of seven of these highly expressed miRNAs and confirmed the sequencing results obtained using the SOLiD platform. Conclusions/Significance In comparison with other tissues, the antrum’s expression profile was unique with respect to the most highly expressed miRNAs, suggesting that this expression profile is specific to stomach antrum tissue. The current study provides a starting point for a more comprehensive understanding of the role of miRNAs in the regulation of the molecular processes of the human stomach. PMID:24647245

  5. Improved beam profile of a 266 nm deep ultraviolet laser employing a multi-mirror-reflected cavity

    NASA Astrophysics Data System (ADS)

    Yang, Houwen; Cheng, Wenyong; Wang, Junhua; Zhang, Yaguang; Wang, Xiaoqian; Zhang, Lijie

    2016-04-01

    A 266 nm deep ultraviolet (DUV) laser with a good Gaussian profile is reported employing a multi-mirror-reflected cavity. A type-I LiB3O5 (LBO) crystal is used to double the fundamental-light (1064 nm) wavelength generated by an actively Q-switched Nd:YVO4 laser with an intra-cavity configuration. A fourth harmonic generation (FHG) wavelength is obtained by a type-I β-BaB2O4 (BBO) crystal. The output power as high as 440 mW at 266 nm is generated under an incident power of 2.26 W at 532 nm, corresponding to the conversion efficiency of 532 nm-266 nm up to 19.5% with a repetition rate of 15 kHz and the pulse duration of 266 nm is 10.7 ns.

  6. Identification of an inflammatory bowel disease patient with a deep vein thrombosis and an altered clot lysis profile.

    PubMed

    Bollen, Lize; Wuyts, Joke; Vermeire, Séverine; Gils, Ann

    2016-03-01

    Patients with inflammatory bowel diseases (IBD), a chronic inflammatory disease characterized by flares and remission, are prone to develop thrombosis. The mechanism behind this prothrombotic state is not completely understood but is definitely multifactorial and linked with excessive inflammation observed in these patients. So far, no biomarker exists to select among IBD patients those with and increased risk for thrombosis. Corticosteroid therapy, given as rescue IBD treatment, is known to increase the thrombotic risk, whereas for antitumor necrosis factor (TNF)-alpha therapy such as infliximab, given to induce and maintain remission in IBD, the results are inconclusive. Here, we describe a 31-year-old IBD patient who developed a deep vein thrombosis. We determined the clot lysis profiles before and after developing thrombosis. We showed that a global functional clot lysis assay can be used as a tool to identify IBD patients who may benefit from thromboprophylactic therapy. PMID:26378816

  7. Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing.

    PubMed

    Dong, Cai-Hua; Li, Chen; Yan, Xiao-Hong; Huang, Shun-Mou; Huang, Jin-Yong; Wang, Li-Jun; Guo, Rui-Xing; Lu, Guang-Yuan; Zhang, Xue-Kun; Fang, Xiao-Ping; Wei, Wen-Hui

    2012-05-01

    Sinapis alba has many desirable agronomic traits including tolerance to drought. In this investigation, we performed the genome-wide transcriptional profiling of S. alba leaves under drought stress and rewatering growth conditions in an attempt to identify candidate genes involved in drought tolerance, using the Illumina deep sequencing technology. The comparative analysis revealed numerous changes in gene expression level attributable to the drought stress, which resulted in the down-regulation of 309 genes and the up-regulation of 248 genes. Gene ontology analysis revealed that the differentially expressed genes were mainly involved in cell division and catalytic and metabolic processes. Our results provide useful information for further analyses of the drought stress tolerance in Sinapis, and will facilitate molecular breeding for Brassica crop plants. PMID:22207172

  8. SinoProbe-02: Deep Seismic Reflection Profiling of the Bangong Suture and Qiangtang terrane in central Tibet

    NASA Astrophysics Data System (ADS)

    Lu, Z.; Chen, C.; Gao, R.; Brown, L.; Xiong, X.; Li, W.; Deng, G.

    2010-12-01

    The Bangong Suture represents the tectonic junction between the two primary terranes of the Tibetan Plateau- the Lhasa block and the Qiangtang terrane.This suture was originally formed during a Jurassic collision of these two terranes,but has retained considerably significance as the suspect surface position of the buried mantle suture between Indian and Asian lithosphere that formed during Himalayan collision.As a corollary,these terranes have been associated with very different styles of mantle tectonics,perhaps as a result of mantle delamination beneath the Qiangtang.There have been a number of attempts to probe the deep structure of both the suture and its flanking terranes with refraction and teleseismic methods.Here we report the results of the first multichannel seismic reflection profile across this critical region.Deep seismic reflection method is internationally recognized as a pioneering technology for imaging crustal details,and it has been successfully applied in Southern Tibetan plateau in early 1990s.From October 2009 to May 2010,SinoProbe collected 310 km of a deep seismic reflection profile crossing BNS,successfully revealing structural details down to the Moho and possible deeper.The profile starts west of Silin Co in the northern Lhasa block,crosses the Bangong-Nujiang suture west of Lunpola,skirts the eastern extension of the central Qiantang anticline and ends at Dogai Coring just of south of Jinsha suture.The survey used explosive sources with variable shot size to insure adequate imaging of both the upper and lower crust.In the southern part of the profile,small shots of 50kg explosive were placed at 30m depth at 250m spacing,augmented by larger shots of 200kg t 50m depth spaced every 1km.In addition,large shots of 1000kg were placed every 50km.In the middle and north part,only 200 kg shots at 500m spacing along with the big shots.A linear array of receivers was used with a group interval of 50 m.The data was acquired by Sercel 408 XL using

  9. Resistivity profile of mountain slopes after deep catastrophic landslides caused by earthquake in Japan

    NASA Astrophysics Data System (ADS)

    Isshiki, Hiromitsu; Takahara, Teruyoshi; Kinoshita, Atsuhiro; Ishiduka, Tadanori

    2014-05-01

    An airborne electromagnetic (AEM) survey can be used to estimate the resistivity structure underground by measuring the electromagnetic induction. An AEM survey can quickly obtain the resistivity in the depth direction for a wide area. The resistivity varies with the type of rock, water saturation, porosity, and clay mineral content. This study targeted the deep catastrophic landslide (DCL) triggered by the Iwate-Miyagi Inland Earthquake in 2008 on the slopes of Mt. Kurikoma, which is an active volcano in northeastern Japan. Fluidized material slid down the slope and struck a small spa, resulting in the loss of human life. When considering mitigation measures, it is important to predict those slopes where DCLs might occur. This study estimated the scale of the slopes at risk of DCLs. First, we conducted a field survey and confirmed the distribution of geological conditions and collapse. Soft altered tuff breccia was spread over the entire slope, while andesite covered a head cliff on the upper slope. It was thought that the DCL occurred at the andesite cap rock, at the slip plane boundary of the andesite and altered tuff breccia. Next, we conducted an AEM survey over a wide area, including the landslide. While the specific resistance of the uncollapsed zone was ≥ 400 Ω-m, that of the collapsed zone was ≤ 100-200 Ω-m. Finally, comparing the results of the field and AEM surveys, the spatial distribution of the specific resistivity roughly corresponded to the underground geological setting, such as andesite cap rock. Based on the distribution of the high-resistivity zone, the slopes at risk of deep-seated landslides and the collapsed soil layer thickness could be estimated. In conclusion, in order to evaluate the risk of a DCL, it is necessary consider underground information. An AEM survey is a practical method for evaluating the risk of a DCL.

  10. Early origins of the Caribbean plate from deep seismic profiles across the Nicaraguan Rise

    NASA Astrophysics Data System (ADS)

    Ott, B.; Mann, W. P.

    2012-12-01

    The offshore Nicaraguan Rise in the maritime zones of Honduras, Jamaica, Nicaragua and Colombia covers a combined area of 500,000 km2, and is one of the least known equatorial Cretaceous-Cenozoic carbonate regions remaining on Earth. The purpose of this study is to describe the Cretaceous to Recent tectonic and stratigraphic history of the deep water Nicaraguan Rise, and to better understand how various types of crustal blocks underlying the Eocene to Recent carbonate cover fused into a single, larger Caribbean plate known today from GPS studies. We interpreted 8700 km of modern, deep-penetration 2D seismic data kindly provided by the oil industry, tied to five wells that penetrated Cretaceous igneous basement. Based on these data, and integration with gravity, magnetic and existing crustal refraction data, we define four crustal provinces for the offshore Nicaraguan Rise: 1) Thicker (15-18 km) Late Cretaceous Caribbean ocean plateau (COP) with rough, top basement surface; 2) normal (6-8 km) Late Cretaceous COP with smooth top basement surface (B") and correlative outcrops in southern Haiti and Jamaica; 3) Precambrian-Paleozoic continental crust (20-22 km thick) with correlative outcrops in northern Central America; and 4) Cretaceous arc crust (>18 km thick) with correlative outcrops in Jamaica. These strongly contrasting basement belts strike northeastward to eastward, and were juxtaposed by latest Cretaceous-Paleogene northward and northwestward thrusting of Caribbean arc over continental crust in Central America, and the western Nicaraguan Rise (84 to 85 degrees west). A large Paleogene to recent, CCW rotation of the Caribbean plate along the Cayman trough faults and into its present day location explains why terranes in Central America and beneath the Nicaraguan Rise have their present, anomalous north-east strike. Continuing, present-day activity on some of these crustal block boundaries is a likely result of intraplate stresses imposed by the surrounding

  11. T2 distribution mapping profiles with phase-encode MRI

    NASA Astrophysics Data System (ADS)

    Petrov, Oleg V.; Ersland, Geir; Balcom, Bruce J.

    2011-03-01

    Two 1-D phase-encode sequences for T2 mapping, namely CPMG-prepared SPRITE and spin-echo SPI, are presented and compared in terms of image quality, accuracy of T2 measurements and the measurement time. The sequences implement two different approaches to acquiring T2-weighted images: in the CPMG-prepared SPRITE, the T2-weighting of magnetization precedes the spatial encoding, while in the spin-echo SPI, the T2-weighting follows the spatial encoding. The sequences are intended primarily for T2 mapping of fluids in porous solids, where using frequency encode techniques may be problematic either due to local gradient distortions or too short T2. Their possible applications include monitoring fluid-flow processes in rocks, cement paste hydration, curing of rubber, filtering paramagnetic impurities and other processes accomplished by changing site-specific T2.

  12. Wrapping-free phase retrieval with applications to interferometry, 3D-shape profiling, and deflectometry.

    PubMed

    Perciante, César D; Strojnik, Marija; Paez, Gonzalo; Di Martino, J Matias; Ayubi, Gastón A; Flores, Jorge L; Ferrari, José A

    2015-04-01

    Phase unwrapping is probably the most challenging step in the phase retrieval process in phase-shifting and spatial-carrier interferometry. Likewise, phase unwrapping is required in 3D-shape profiling and deflectometry. In this paper, we present a novel phase retrieval method that completely sidesteps the phase unwrapping process, significantly eliminating the guessing in phase reconstruction and thus decreasing the time data processing. The proposed wrapping-free method is based on the direct integration of the spatial derivatives of the interference patterns under the single assumption that the phase is continuous. This assumption is valid in most physical applications. Validation experiments are presented confirming the robustness of the proposed method. PMID:25967217

  13. A green deep eutectic solvent-based aqueous two-phase system for protein extracting.

    PubMed

    Xu, Kaijia; Wang, Yuzhi; Huang, Yanhua; Li, Na; Wen, Qian

    2015-03-15

    As a new type of green solvent, deep eutectic solvent (DES) has been applied for the extraction of proteins with an aqueous two-phase system (ATPS) in this work. Four kinds of choline chloride (ChCl)-based DESs were synthesized to extract bovine serum albumin (BSA), and ChCl-glycerol was selected as the suitable extraction solvent. Single factor experiments have been done to investigate the effects of the extraction process, including the amount of DES, the concentration of salt, the mass of protein, the shaking time, the temperature and PH value. Experimental results show 98.16% of the BSA could be extracted into the DES-rich phase in a single-step extraction under the optimized conditions. A high extraction efficiency of 94.36% was achieved, while the conditions were applied to the extraction of trypsin (Try). Precision, repeatability and stability experiments were studied and the relative standard deviations (RSD) of the extraction efficiency were 0.4246% (n=3), 1.6057% (n=3) and 1.6132% (n=3), respectively. Conformation of BSA was not changed during the extraction process according to the investigation of UV-vis spectra, FT-IR spectra and CD spectra of BSA. The conductivity, dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to explore the mechanism of the extraction. It turned out that the formation of DES-protein aggregates play a significant role in the separation process. All the results suggest that ChCl-based DES-ATPS are supposed to have the potential to provide new possibilities in the separation of proteins. PMID:25732422

  14. Phases and Interfaces from Real Space Atomically Resolved Data: Physics-Based Deep Data Image Analysis.

    PubMed

    Vasudevan, Rama K; Ziatdinov, Maxim; Jesse, Stephen; Kalinin, Sergei V

    2016-09-14

    Advances in electron and scanning probe microscopies have led to a wealth of atomically resolved structural and electronic data, often with ∼1-10 pm precision. However, knowledge generation from such data requires the development of a physics-based robust framework to link the observed structures to macroscopic chemical and physical descriptors, including single phase regions, order parameter fields, interfaces, and structural and topological defects. Here, we develop an approach based on a synergy of sliding window Fourier transform to capture the local analog of traditional structure factors combined with blind linear unmixing of the resultant 4D data set. This deep data analysis is ideally matched to the underlying physics of the problem and allows reconstruction of the a priori unknown structure factors of individual components and their spatial localization. We demonstrate the principles of this approach using a synthetic data set and further apply it for extracting chemical and physically relevant information from electron and scanning tunneling microscopy data. This method promises to dramatically speed up crystallographic analysis in atomically resolved data, paving the road toward automatic local structure-property determinations in crystalline and quasi-ordered systems, as well as systems with competing structural and electronic order parameters. PMID:27517608

  15. Deep-blue supercontinnum sources with optimum taper profiles--verification of GAM.

    PubMed

    Sørensen, S T; Møller, U; Larsen, C; Moselund, P M; Jakobsen, C; Johansen, J; Andersen, T V; Thomsen, C L; Bang, O

    2012-05-01

    We use an asymmetric 2 m draw-tower photonic crystal fiber taper to demonstrate that the taper profile needs careful optimisation if you want to develop a supercontinuum light source with as much power as possible in the blue edge of the spectrum. In particular we show, that for a given taper length, the downtapering should be as long as possible. We argue how this may be explained by the concept of group-acceleration mismatch (GAM) and we confirm the results using conventional symmetrical short tapers made on a taper station, which have varying downtapering lengths. PMID:22565689

  16. Transcurrent nature of the Teisseyre-Tornquist Zone in Central Europe: results of the POLCRUST-01 deep reflection seismic profile

    NASA Astrophysics Data System (ADS)

    Narkiewicz, M.; Maksym, A.; Malinowski, M.; Grad, M.; Guterch, A.; Petecki, Z.; Probulski, J.; Janik, T.; Majdański, M.; Środa, P.; Czuba, W.; Gaczyński, E.; Jankowski, L.

    2015-04-01

    Teisseyre-Tornquist Zone (TTZ) corresponds to a crustal boundary between the Precambrian East European Platform (EEP) and the Palaeozoic West European Platform. Although the zone has been controlling Phanerozoic evolution of large parts of Central Europe, its course, geometry and origin are still poorly constrained. Deep reflection seismic profile POLCRUST-01, recently acquired in SE Poland, for the first time allowed a precise comparison of the Ediacaran and later tectonic patterns to the deep crustal features of the TTZ and adjacent areas. The TTZ corresponds to the subvertical Tomaszów Fault separating the Radom-Kraśnik Elevation, composed of the typical EEP crust, from the Biłgoraj-Narol Block (BNB) in the SW, with a thinned crystalline basement showing affinities to the EEP crust. The BNB is a part of the larger Caledonian Łysogóry Terrane as evidenced by its Lower Palaeozoic stratigraphy and gravity data. Thus, for the first time, the proximal Baltican affinity of this unit has been documented unambiguously. The Łysogóry Terrane is delimited from the SW by the subvertical Cieszanów Fault Zone, corresponding to the Holy Cross Suture. The adjacent Małopolska Terrane is characterized by a distinct Early Palaeozoic stratigraphy, and lower-middle crust exhibiting SW-dipping reflective packages interpreted as NE-verging thrust and shear zones of a Neoproterozoic orogen. The observations from the POLCRUST-01 profile and regional comparisons indicate that the TTZ is a major Caledonian transcurrent zone between Poland and East Romania. In central Poland, the TTZ likely forms a narrow subvertical contact between the EEP and a proximal Kuiavia Terrane, as constrained by the deep refraction seismic data. To the NW, the zone extends towards the Pomeranian part of the Caledonide fold-and-thrust belt related to the Avalonia-Baltica collision zone (Thor Suture). South-east of Poland the TTZ corresponds to the Rava Ruska Fault Zone established as a Caledonian suture

  17. Reconstructing conjugate margins of the Canada-Amerasian basin: New tectonic constraints from deep seismic data and gravity profiles

    NASA Astrophysics Data System (ADS)

    Helwig, J.; Ady, B.; Kumar, N.; Granath, J. W.; Dinkelman, M. G.; Bird, D. E.; Emmet, P. A.

    2010-12-01

    Over the past 5 years, decreasing sea ice and increasing scientific and economic interest in the Arctic have prompted new geological and geophysical studies that advance knowledge of the northern continental margins of North America. We report here on ArcticSPAN™ 40-km deep, PSDM (Pre-Stack Depth Migrated) marine seismic reflection profiles and gravity data from the Beaufort Sea of Canada and the US Chukchi Sea that constrain the position of the continent-ocean boundary and the relict spreading center of the Canada Basin, displaying significant variations in the orientation, geometry and deep crustal structure of the passive margin facing the Arctic Ocean. In the Canadian Beaufort Sea three distinct segments of the margin correspond to contrasts of pre-rift foundations: 1. the rifted, rotated Arctic Alaska Terrane west of the Mackenzie Delta (Beaufort segment); 2. the transform-faulted Laurentian crust of the Tuktoyaktuk margin (Tuk segment); and, 3. the rifted Laurentian crust of the Banks Island segment. The thick late Mesozoic-Cenozoic clastic prism of the continental margin was centered in the Mackenzie delta area by Mesozoic rifting of the Canada Basin. The northerly Paleocene-Miocene sweep of Cordilleran deformation modified the passive margin, overprinting the offshore Mackenzie Delta. The interpreted tectonic architecture of the three segments of the Beaufort passive margin demonstrates their distinct roles in opening of the Canada Basin. Two conjugate rifted margin segments (Beaufort and Banks Island) and a linking transform fault margin (Tuk) formed during the separation of the Arctic Alaska Terrane from northwestern Laurentia, in accord with a Jurassic-Aptian rotational model of Canada Basin opening. But the orientation of the Tuk transform segment indicates that a single pole of rotation cannot describe the opening of the basin. Additional seismic profiles from investigations of the Chukchi Sea margin display passive margin structures and rift to pre

  18. Doppler Radar Profiler for Launch Winds at the Kennedy Space Center (Phase 1a)

    NASA Technical Reports Server (NTRS)

    Murri, Daniel G.

    2011-01-01

    The NASA Engineering and Safety Center (NESC) received a request from the, NASA Technical Fellow for Flight Mechanics at Langley Research Center (LaRC), to develop a database from multiple Doppler radar wind profiler (DRWP) sources and develop data processing algorithms to construct high temporal resolution DRWP wind profiles for day-of-launch (DOL) vehicle assessment. This document contains the outcome of Phase 1a of the assessment including Findings, Observations, NESC Recommendations, and Lessons Learned.

  19. Metabolomic profiling reveals deep chemical divergence between two morphotypes of the zoanthid Parazoanthus axinellae

    NASA Astrophysics Data System (ADS)

    Cachet, Nadja; Genta-Jouve, Grégory; Ivanisevic, Julijana; Chevaldonné, Pierre; Sinniger, Frédéric; Culioli, Gérald; Pérez, Thierry; Thomas, Olivier P.

    2015-02-01

    Metabolomics has recently proven its usefulness as complementary tool to traditional morphological and genetic analyses for the classification of marine invertebrates. Among the metabolite-rich cnidarian order Zoantharia, Parazoanthus is a polyphyletic genus whose systematics and phylogeny remain controversial. Within this genus, one of the most studied species, Parazoanthus axinellae is prominent in rocky shallow waters of the Mediterranean Sea and the NE Atlantic Ocean. Although different morphotypes can easily be distinguished, only one species is recognized to date. Here, a metabolomic profiling approach has been used to assess the chemical diversity of two main Mediterranean morphotypes, the ``slender'' and ``stocky'' forms of P. axinellae. Targeted profiling of their major secondary metabolites revealed a significant chemical divergence between the morphotypes. While zoanthoxanthin alkaloids and ecdysteroids are abundant in both morphs, the ``slender'' morphotype is characterized by the presence of additional and bioactive 3,5-disubstituted hydantoin derivatives named parazoanthines. The absence of these specific compounds in the ``stocky'' morphotype was confirmed by spatial and temporal monitoring over an annual cycle. Moreover, specimens of the ``slender'' morphotype are also the only ones found as epibionts of several sponge species, particularly Cymbaxinella damicornis thus suggesting a putative ecological link.

  20. Metabolomic profiling reveals deep chemical divergence between two morphotypes of the zoanthid Parazoanthus axinellae

    PubMed Central

    Cachet, Nadja; Genta-Jouve, Grégory; Ivanisevic, Julijana; Chevaldonné, Pierre; Sinniger, Frédéric; Culioli, Gérald; Pérez, Thierry; Thomas, Olivier P.

    2015-01-01

    Metabolomics has recently proven its usefulness as complementary tool to traditional morphological and genetic analyses for the classification of marine invertebrates. Among the metabolite-rich cnidarian order Zoantharia, Parazoanthus is a polyphyletic genus whose systematics and phylogeny remain controversial. Within this genus, one of the most studied species, Parazoanthus axinellae is prominent in rocky shallow waters of the Mediterranean Sea and the NE Atlantic Ocean. Although different morphotypes can easily be distinguished, only one species is recognized to date. Here, a metabolomic profiling approach has been used to assess the chemical diversity of two main Mediterranean morphotypes, the “slender” and “stocky” forms of P. axinellae. Targeted profiling of their major secondary metabolites revealed a significant chemical divergence between the morphotypes. While zoanthoxanthin alkaloids and ecdysteroids are abundant in both morphs, the “slender” morphotype is characterized by the presence of additional and bioactive 3,5-disubstituted hydantoin derivatives named parazoanthines. The absence of these specific compounds in the “stocky” morphotype was confirmed by spatial and temporal monitoring over an annual cycle. Moreover, specimens of the “slender” morphotype are also the only ones found as epibionts of several sponge species, particularly Cymbaxinella damicornis thus suggesting a putative ecological link. PMID:25655432

  1. Detection of Alzheimer’s disease amyloid-beta plaque deposition by deep brain impedance profiling

    NASA Astrophysics Data System (ADS)

    Béduer, Amélie; Joris, Pierre; Mosser, Sébastien; Fraering, Patrick C.; Renaud, Philippe

    2015-04-01

    Objective. Alzheimer disease (AD) is the most common form of neurodegenerative disease in elderly people. Toxic brain amyloid-beta (Aß) aggregates and ensuing cell death are believed to play a central role in the pathogenesis of the disease. In this study, we investigated if we could monitor the presence of these aggregates by performing in situ electrical impedance spectroscopy measurements in AD model mice brains. Approach. In this study, electrical impedance spectroscopy measurements were performed post-mortem in APPPS1 transgenic mice brains. This transgenic model is commonly used to study amyloidogenesis, a pathological hallmark of AD. We used flexible probes with embedded micrometric electrodes array to demonstrate the feasibility of detecting senile plaques composed of Aß peptides by localized impedance measurements. Main results. We particularly focused on deep brain structures, such as the hippocampus. Ex vivo experiments using brains from young and old APPPS1 mice lead us to show that impedance measurements clearly correlate with the percentage of Aβ plaque load in the brain tissues. We could monitor the effects of aging in the AD APPPS1 mice model. Significance. We demonstrated that a localized electrical impedance measurement constitutes a valuable technique to monitor the presence of Aβ-plaques, which is complementary with existing imaging techniques. This method does not require prior Aβ staining, precluding the risk of variations in tissue uptake of dyes or tracers, and consequently ensuring reproducible data collection.

  2. Speedup properties of phases in the execution profile of distributed parallel programs

    SciTech Connect

    Carlson, B.M.; Wagner, T.D.; Dowdy, L.W.; Worley, P.H.

    1992-08-01

    The execution profile of a distributed-memory parallel program specifies the number of busy processors as a function of time. Periods of homogeneous processor utilization are manifested in many execution profiles. These periods can usually be correlated with the algorithms implemented in the underlying parallel code. Three families of methods for smoothing execution profile data are presented. These approaches simplify the problem of detecting end points of periods of homogeneous utilization. These periods, called phases, are then examined in isolation, and their speedup characteristics are explored. A specific workload executed on an Intel iPSC/860 is used for validation of the techniques described.

  3. A gravity model of the deep structure of South Caspian Basin along submeridional profile Alborz-Absheron Sill

    NASA Astrophysics Data System (ADS)

    Kadirov, F. A.; Gadirov, A. H.

    2014-03-01

    The South-Caspian Basin (SCB) underlies the southern part of the Caspian Sea, between the ranges of the eastern Greater Caucasus, Talysh, Alborz, and Kopet Dagh. A 2-D regional gravity model along a profile from the Alborz Mountains to the Absheron Ridge has been constructed, constrained by deep (20 s TWT) seismic reflection data. The deep structure model has been evaluated in terms of earthquake focal mechanisms and GPS velocity data to elucidate active tectonic processes and the geodynamic evolution of the SCB. We believe that the rapid increase in the thickness of Mesozoic sediments along the profile from ~ 8 km in the middle part of the profile up to ~ 15 km in the area of the Absheron-Ridge can be explained by inherent basin geometry created by thermal subsidence followed by sediment loading as well as additional effect of tectonic related shortening of sedimentary succession. Near the boundary of oceanic and continental crust in the northern SCB, flexure of oceanic crust is inferred from the observed seismic data and gravity modeling, most probably connected to ongoing subduction of lithosphere of the South Caspian underneath the Scythian Plate of the Mid-Caspian. Subduction beneath the Absheron Ridge is accompanied by the delamination of sediments from the oceanic crust (“basaltic” layer) and creation of accretionary wedge in the overlaying sedimentary succession. The focal mechanisms of the larger earthquakes (M > 6) occurring along the northern boundary of the SCB show steep normal-type faulting above the bend of the downgoing slab while, along the southern boundary, thrust faults are inferred. Some thrust-type earthquakes near the northern boundary occur in the lower crust or uppermost mantle and may be associated with compression in the lower part of the brittle lithosphere due to plate flexure. Displacements measured along the coastline of the Caspian Sea by GPS are consistent with the direction of potential oblique subduction of oceanic crust of

  4. Phase compensation with fiber optic surface profile acquisition and reconstruction system

    NASA Astrophysics Data System (ADS)

    Bo, En; Duan, Fajie; Feng, Fan; Lv, Changrong; Xiao, Fu; Huang, Tingting

    2015-02-01

    A fiber-optic sinusoidal phase modulating (SPM) interferometer was proposed for the acquisition and reconstruction of three-dimensional (3-D) surface profile. Sinusoidal phase modulation was induced by controlling the injection current of light source. The surface profile was constructed on the basis of fringe projection. Fringe patterns are vulnerable to external disturbances such as mechanical vibration and temperature fluctuation, which cause phase drift in the interference signal and decrease measuring accuracy. A closed-loop feedback phase compensation system was built. In the subsystem, the initial phase of the interference signal, which was caused by the initial optical path difference between interference arms, could be demodulated using phase generated carrier (PGC) method and counted out using coordinated rotation digital computer (CORDIC) , then a compensation voltage was generated for the PZT driver. The bias value of external disturbances superimposed on fringe patterns could be reduced to about 50 mrad, and the phase stability for interference fringes was less than 6 mrad. The feasibility for real-time profile measurement has been verified.

  5. Improved optical profiling using the spectral phase in spectrally resolved white-light interferometry

    SciTech Connect

    Debnath, Sanjit Kumar; Kothiyal, Mahendra Prasad

    2006-09-20

    In spectrally resolved white-light interferometry (SRWLI), the white-light interferogram is decomposed into its monochromatic constituent. The phase of the monochromatic constituents can be determined using a phase-shifting technique over a range of wavelengths. These phase value shave fringe order ambiguity. However, the variation of the phase with respect to the wavenumber is linear and its slope gives the absolute value of the optical-path difference. Since the path difference is related to the height of the test object at a point, a line profile can be determined without ambiguity. The slope value, though less precise helps us determine the fringe order. The fringe order combined with the monochromatic phase value gives the absolute profile, which has the precision of phase-shifting interferometry. The presence of noise in the phase may lead to the misidentification of fringe order, which in turn gives unnecessary jumps in the precise profile. The experimental details of measurement on standard samples with SRWLI are discussed in this paper.

  6. Expression profiling of Drosophila mitochondrial genes via deep mRNA sequencing

    PubMed Central

    Torres, Tatiana Teixeira; Dolezal, Marlies; Schlötterer, Christian; Ottenwälder, Birgit

    2009-01-01

    Mitochondria play an essential role in several cellular processes. Nevertheless, very little is known about patterns of gene expression of genes encoded by the mitochondrial DNA (mtDNA). In this study, we used next-generation sequencing (NGS) for transcription profiling of genes encoded in the mitochondrial genome of Drosophila melanogaster and D. pseudoobscura. The analysis of males and females in both species indicated that the expression pattern was conserved between the two species, but differed significantly between both sexes. Interestingly, mRNA levels were not only different among genes encoded by separate transcription units, but also showed significant differences among genes located in the same transcription unit. Hence, mRNA abundance of genes encoded by mtDNA seems to be heavily modulated by post-transcriptional regulation. Finally, we also identified several transcripts with a noncanonical structure, suggesting that processing of mitochondrial transcripts may be more complex than previously assumed. PMID:19843606

  7. Deep seismic studies of conjugate profiles from the Nova Scotia - Moroccan and the Liguro-Provencal margin pairs

    NASA Astrophysics Data System (ADS)

    Klingelhoefer, F.; Biari, Y.; Sahabi, M.; Aslanian, D.; Philippe, S.; Schnabel, M.; Moulin, M.; Louden, K. E.; Funck, T.; Reichert, C. J.

    2014-12-01

    The structure of conjugate passive margins provides information about rifting styles, opening of an ocean and formation of it's associated sedimentary basins. In order to distinguish between tectonic inheritance and structures directly related to rifting of passive margins conjugate profiles have to be acquired on margins on diverse locations and different ages. In this study we use new and existing reflection and wide-angle seismic data from two margin pairs, the 200 Ma year old Nova-Scotia - Morocco margin pair and the only 20 Ma Gulf of Lions - Sardinia margin pair. On both margin pairs wide-angle seismic data combined with reflection seismic data were acquired on conjugate profiles on sea and extended on land. Forward modelling of the deep crustal structure along the four transects indicates that a high velocity zone (HVZ) (> 7.2 km/s) is present at the base of the lower crust on all four margins along the ocean-continental transition zone (OCT). This may represent either exhumed upper mantle material or injection of upper mantle material into proto-oceanic crust at the onset of sea-floor spreading. However the width of the HVZ might strongly differ between conjugates, which may be the result of tectonic inheritance, for example the presence of ancient subduction zones or orogens. Both margin pairs show a similar unthinned continental crustal thickness. Crustal thinning and upper-to-lower crustal thickness vary between margin pairs, but remain nearly symmetric on conjugate profiles and might therefore depend on the structure and mechanical properties of the original continental crust. For the Mediterranean margin pair, the oceanic crust is similar on both sides, with a thickness of only 4-5 km. For the Atlantic margin pair, oceanic crustal thickness is higher on the Moroccan Margin, a fact that can be explained by either asymmetric spreading or by the volcanic underplating, possibly originating from the Canary Hot Spot.

  8. SHARAKU: an algorithm for aligning and clustering read mapping profiles of deep sequencing in non-coding RNA processing

    PubMed Central

    Tsuchiya, Mariko; Amano, Kojiro; Abe, Masaya; Seki, Misato; Hase, Sumitaka; Sato, Kengo; Sakakibara, Yasubumi

    2016-01-01

    Motivation: Deep sequencing of the transcripts of regulatory non-coding RNA generates footprints of post-transcriptional processes. After obtaining sequence reads, the short reads are mapped to a reference genome, and specific mapping patterns can be detected called read mapping profiles, which are distinct from random non-functional degradation patterns. These patterns reflect the maturation processes that lead to the production of shorter RNA sequences. Recent next-generation sequencing studies have revealed not only the typical maturation process of miRNAs but also the various processing mechanisms of small RNAs derived from tRNAs and snoRNAs. Results: We developed an algorithm termed SHARAKU to align two read mapping profiles of next-generation sequencing outputs for non-coding RNAs. In contrast with previous work, SHARAKU incorporates the primary and secondary sequence structures into an alignment of read mapping profiles to allow for the detection of common processing patterns. Using a benchmark simulated dataset, SHARAKU exhibited superior performance to previous methods for correctly clustering the read mapping profiles with respect to 5′-end processing and 3′-end processing from degradation patterns and in detecting similar processing patterns in deriving the shorter RNAs. Further, using experimental data of small RNA sequencing for the common marmoset brain, SHARAKU succeeded in identifying the significant clusters of read mapping profiles for similar processing patterns of small derived RNA families expressed in the brain. Availability and Implementation: The source code of our program SHARAKU is available at http://www.dna.bio.keio.ac.jp/sharaku/, and the simulated dataset used in this work is available at the same link. Accession code: The sequence data from the whole RNA transcripts in the hippocampus of the left brain used in this work is available from the DNA DataBank of Japan (DDBJ) Sequence Read Archive (DRA) under the accession number DRA

  9. Reproducibility of trace element profiles in a specimen of the deep-water bamboo coral Keratoisis sp.

    NASA Astrophysics Data System (ADS)

    Sinclair, Daniel J.; Williams, B.; Allard, G.; Ghaleb, B.; Fallon, S.; Ross, S. W.; Risk, M.

    2011-09-01

    Bamboo corals (Order Gorgonacea, Family Isididae) are attractive prospects for deep-sea paleoceanographic reconstruction, capturing trace elements in their calcitic skeletons that may serve as environmental proxies with subdecadal resolution over multi-century timescales. We study the reproducibility and fidelity of trace-element profiles (Ba, Mg, Sr, Mn, U, Pb) in a 420-year-old specimen of the bamboo coral Keratoisis sp. from the SE USA. Using laser-ablation ICP-MS to obtain multiple replicate profiles, we use spectral techniques to distinguish noise and irreproducible variations from fully reproducible geochemical fluctuations that are candidates for environmental signals. By quantifying variability between profiles, we assess the fidelity with which the corals potentially record environmental information. Barium is the most reproducible element in the skeleton, with large fluctuations along different growth radii reproducing to within 4%. Both Mg and Sr have very uniform levels within the coral, but display low-amplitude irreproducible variations that might represent an internal biological process. In the case of Mg, which has been proposed as a paleotemperature proxy, this irreproducibility would represent an intrinsic uncertainty of ˜±0.1 to 0.4 °C. Both Mn and Pb contain some irreproducibility superimposed upon broad reproducible profiles that may be environmental signals. Some of the irreproducible Pb fluctuations correlate with cracks and dark bands in the sample suggesting detrital or surface contamination. Uranium displays large amplitude variations which are not reproducible along different radii. This suggests that uranium cannot be used for paleoenvironmental reconstruction, and may show signs of early diagenesis - a possibility that could complicate attempts to date young Keratoisis sp. samples by U-series geochemistry. The highly reproducible Ba signal allows precise alignment of profiles and thus we can show that growth rate along one radius can

  10. Magnetic solid-phase extraction of protein with deep eutectic solvent immobilized magnetic graphene oxide nanoparticles.

    PubMed

    Xu, Kaijia; Wang, Yuzhi; Ding, Xueqin; Huang, Yanhua; Li, Na; Wen, Qian

    2016-02-01

    As a new type of green solvent, four kinds of choline chloride (ChCl)-based deep eutectic solvents (DESs) have been synthesized, and then a core-shell structure magnetic graphene oxide (Fe3O4-NH2@GO) nanoparticles have been prepared and coated with the ChCl-based DESs. Magnetic solid-phase extraction (MSPE) based Fe3O4-NH2@GO@DES was studied for the first time for the extraction of proteins. The characteristic results of vibrating sample magnetometer (VSM), X-ray diffraction (XRD), Fourier transform infrared spectrometry (FT-IR), thermal gravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM) indicated the successful preparation of Fe3O4-NH2@GO@DES. The concentrations of proteins in studies were determined by a UV-vis spectrophotometer. The advantages of Fe3O4-NH2@GO@DES in protein extraction were compared with Fe3O4-NH2@GO and Fe3O4-NH2, and Fe3O4-NH2@GO@ChCl-glycerol was selected as the suitable extraction solvent. The influence factors of the extraction process such as the pH value, the temperature, the extraction time, the concentration of protein and the amount of Fe3O4-NH2@GO@ChCl-glycerol were evaluated. Desorption experimental result showed 98.73% of BSA could be eluted from the solid extractant with 0.1 mol/L Na2HPO4 solution contained 1 mol/L NaCl. Besides, the conformation of BSA was not changed during the elution by the investigation of circular dichromism (CD) spectra. Furthermore, the analysis of real sample demonstrated that the prepared magnetic nanoparticles did have extraction ability on proteins in bovine whole blood. PMID:26653436

  11. Deep-sequence profiling of miRNAs and their target prediction in Monotropa hypopitys.

    PubMed

    Shchennikova, Anna V; Beletsky, Alexey V; Shulga, Olga A; Mazur, Alexander M; Prokhortchouk, Egor B; Kochieva, Elena Z; Ravin, Nikolay V; Skryabin, Konstantin G

    2016-07-01

    Myco-heterotroph Monotropa hypopitys is a widely spread perennial herb used to study symbiotic interactions and physiological mechanisms underlying the development of non-photosynthetic plant. Here, we performed, for the first time, transcriptome-wide characterization of M. hypopitys miRNA profile using high throughput Illumina sequencing. As a result of small RNA library sequencing and bioinformatic analysis, we identified 55 members belonging to 40 families of known miRNAs and 17 putative novel miRNAs unique for M. hypopitys. Computational screening revealed 206 potential mRNA targets for known miRNAs and 31 potential mRNA targets for novel miRNAs. The predicted target genes were described in Gene Ontology terms and were found to be involved in a broad range of metabolic and regulatory pathways. The identification of novel M. hypopitys-specific miRNAs, some with few target genes and low abundances, suggests their recent evolutionary origin and participation in highly specialized regulatory mechanisms fundamental for non-photosynthetic biology of M. hypopitys. This global analysis of miRNAs and their potential targets in M. hypopitys provides a framework for further investigation of miRNA role in the evolution and establishment of non-photosynthetic myco-heterotrophs. PMID:27097902

  12. Initiation and evolution of the Oligo-Miocene rift basins of southwestern Europe: Contribution of deep seismic reflection profiling

    NASA Astrophysics Data System (ADS)

    Bois, C.

    1993-11-01

    Southwestern European Oligo-Miocene rift basins have recently been investigated by deep seismic reflection profiling. The study of these data, together with other geophysical and geological data, shows that the rifts, which run from the Rhinegraben to the western Mediterranean, do not form a single clearcut system. The N-trending rifts (Rhinegraben, Bresse and Limagne) were developed on a cold and rigid lithosphere affected by the Alpine collision. The NE-trending rifts (southeastern France, Gulf of Lions and Valencia Trough) were formed slightly later in a backarc basin associated with an active segment of the European-Iberian plate that was heated, affected by widespread calcalkaline volcanism and probably weakened. All the southwestern European rifts and basins together may, however, be related to a common heritage represented by the boundary between the European-Iberian and African-Apulian plates that was created in the Jurassic with the initiation of the Tethys Ocean. The present features of the southwestern European Oligo-Miocène rift basins may be explained by a combination of three geodynamic mechanisms: mechanical stretching of the lithosphere, active mantle uplifting, and subordinate lithospheric flexuring. All the rifts were probably initiated by passive stretching. A systematic discrepancy between stretching derived from fault analysis and attenuation of the crust has been observed in all the rifts. This suggests that these rifts were subsequently reworked by one or several active mantle upwelling events associated with late shoulder uplift, asthenosphere upwelling and anomalous P-wave velocities in the lowermost crust and the uppermost mantle. Crustal attenuation may have been achieved by mantle intrusion, metamorphism of the deep crust and/or its delamination. Some of the rifts were affected by lithospheric flexuring. Combinations, in various proportions, of a small number of geodynamic mechanisms probably controlled many basins in the world. This

  13. SPSP Phase III Recruiting, Selecting, and Developing Secure Power Systems Professionals. Job Profiles

    SciTech Connect

    O'Neil, Lori Ross; Conway, T. J.; Tobey, D. H.; Greitzer, Frank L.; Dalton, Angela C.; Pusey, Portia K.

    2015-03-01

    The Secure Power Systems Professional Phase III final report was released last year which an appendix of Job Profiles. This new report is that appendix broken out as a standalone document to assist utilities in recruiting and developing Secure Power Systems Professionals at their site.

  14. National Environmental/Energy Workforce Assessment, Phase II, Post-Secondary Education Profile: Abstract.

    ERIC Educational Resources Information Center

    National Field Research Center Inc., Iowa City, IA.

    The material presented in this booklet represents a condensation of materials developed under the Post-Secondary Education Profile phase of the National Environmental/Energy Workforce Assessment. The final report of the study consisted of 16 volumes which are summarized. The following topics are covered: air, energy, noise, pesticides, potable…

  15. Surface energy from order parameter profile: At the QCD phase transition

    NASA Technical Reports Server (NTRS)

    Frei, Z.; Patkos, A.

    1989-01-01

    The order parameter profile between coexisting confined and plasma regions at the quantum chromodynamic (QCD) phase transition is constructed. The dimensionless combination of the surface energy (Sigma) and the correlation length (Zeta) is estimated to be Sigma Zeta 3 approximately equals 0.8.

  16. Non-power law behavior of the radial profile of phase-space density of halos

    SciTech Connect

    Popolo, A. Del

    2011-07-01

    We study the pseudo phase-space density, ρ(r)/σ{sup 3}(r), of ΛCDM dark matter halos with and without baryons (baryons+DM, and pure DM), by using the model introduced in Del Popolo (2009), which takes into account the effect of dynamical friction, ordered and random angular momentum, baryons adiabatic contraction and dark matter baryons interplay. We examine the radial dependence of ρ(r)/σ{sup 3}(r) over 9 orders of magnitude in radius for structures on galactic and cluster of galaxies scales. We find that ρ(r)/σ{sup 3}(r) is approximately a power-law only in the range of halo radius resolved by current simulations (down to 0.1% of the virial radius) while it has a non-power law behavior below the quoted scale, with inner profiles changing with mass. The non-power-law behavior is more evident for halos constituted both of dark matter and baryons while halos constituted just of dark matter and with angular momentum chosen to reproduce a Navarro-Frenk-White (NFW) density profile, are characterized by an approximately power-law behavior. The results of the present paper lead to conclude that density profiles of the NFW type are compatible with a power-law behavior of ρ(r)/σ{sup 3}(r), while those flattening to the halo center, like those found in Del Popolo (2009) or the Einasto profile, or the Burkert profile, cannot produce radial profile of the pseudo-phase-space density that are power-laws at all radii. The results argue against universality of the pseudo phase-space density and as a consequence argue against universality of density profiles constituted by dark matter and baryons as also discussed in Del Popolo (2009)

  17. Shaping the beam profile of an elliptical Gaussian beam by an elliptical phase aperture

    NASA Astrophysics Data System (ADS)

    Wen, Wei; Wu, Gaofeng; Song, Kehui; Dong, Yiming

    2013-03-01

    Based on the generalized Collins integral formula, an analytical paraxial propagation formula for an elliptical Gaussian beam (EGB) passing through an astigmatic ABCD optical system with an elliptical phase aperture is derived by use of a tensor method. As an application example, we study the propagation properties of an EGB passing through an elliptical aperture in free space. It is found that the elliptical phase aperture can be used for shaping the beam profile of an EGB, which is useful in many applications, such as free space optical communication and material thermal processing. The elliptical phase aperture induced changes of the propagation factors of an EGB are also analyzed.

  18. Scattering in the atmosphere of Venus. III - Line profiles and phase curves for Rayleigh scattering

    NASA Technical Reports Server (NTRS)

    Young, L. D. G.; Kattawar, G. W.

    1977-01-01

    Spectral line profiles, curves of growth, and curves for the equivalent width of a line as a function of Venus phase angle have been computed for a Rayleigh scattering cloud and compared with those for a cloud of isotropic scatterers. The results are very similar for the two kinds of scattering, with the exception of the curves of equivalent width as a function of Venus phase angle. These latter curves exhibit the 'inverse phase effect' and rule out a scale height of the clouds much less than half the scale height of the gas. The optical depth of the clouds is approximately 100.

  19. Scattering in the atmosphere of Venus. Line profiles and phase curves for Rayleigh scattering

    NASA Technical Reports Server (NTRS)

    Young, L. D. G.; Kattawar, G. W.

    1976-01-01

    Spectral line profiles, curves of growth, and curves for the equivalent width of a line as a function of Venus phase angle are computed for a Rayleigh scattering cloud and compared with those for a cloud of isotropic scatterers. The results are similar for the two kinds of scattering, with the exception for the curves of equivalent width as a function of Venus phase angle. These latter curves exhibit the inverse phase effect and rule out the possibility that the scale height of the clouds can be much less than half the scale height of the gas.

  20. Uncovering a Salt Giant. Deep-Sea Record of Mediterranean Messinian Events (DREAM) multi-phase drilling project

    NASA Astrophysics Data System (ADS)

    Camerlenghi, Angelo; Aoisi, Vanni; Lofi, Johanna; Hübscher, Christian; deLange, Gert; Flecker, Rachel; Garcia-Castellanos, Daniel; Gorini, Christian; Gvirtzman, Zohar; Krijgsman, Wout; Lugli, Stefano; Makowsky, Yizhaq; Manzi, Vinicio; McGenity, Terry; Panieri, Giuliana; Rabineau, Marina; Roveri, Marco; Sierro, Francisco Javier; Waldmann, Nicolas

    2014-05-01

    In May 2013, the DREAM MagellanPlus Workshop was held in Brisighella (Italy). The initiative builds from recent activities by various research groups to identify potential sites to perform deep-sea scientific drilling in the Mediterranean Sea across the deep Messinian Salinity Crisis (MSC) sedimentary record. In this workshop three generations of scientists were gathered: those who participated in formulation of the deep desiccated model, through DSDP Leg 13 drilling in 1973; those who are actively involved in present-day MSC research; and the next generation (PhD students and young post-docs). The purpose of the workshop was to identify locations for multiple-site drilling (including riser-drilling) in the Mediterranean Sea that would contribute to solve the several open questions still existing about the causes, processes, timing and consequences at local and planetary scale of an outstanding case of natural environmental change in the recent Earth history: the Messinian Salinity Crisis in the Mediterranean Sea. The product of the workshop is the identification of the structure of an experimental design of site characterization, riser-less and riser drilling, sampling, measurements, and down-hole analyses that will be the core for at least one compelling and feasible multiple phase drilling proposal. Particular focus has been given to reviewing seismic site survey data available from different research groups at pan-Mediterranean basin scale, to the assessment of additional site survey activity including 3D seismics, and to ways of establishing firm links with oil and gas industry. The scientific community behind the DREAM initiative is willing to proceed with the submission to IODP of a Multi-phase Drilling Project including several drilling proposals addressing specific drilling objectives, all linked to the driving objectives of the MSC drilling and understanding . A series of critical drilling targets were identified to address the still open questions

  1. Vertical profiles of aerosol radiative forcing - a comparison of AEROCOM phase 2 model submissions

    NASA Astrophysics Data System (ADS)

    Samset, B. H.; Myhre, G.

    2012-04-01

    Aerosols in the earth's atmosphere affect the radiation balance of the planet. The radiative forcing (RF) induced by a given aerosol burden is however sensitive to its vertical density profile, in addition to aerosol optical properties, cloud distributions and surface albedo. Differences in vertical profiles are thought to be among the causes for the large intermodel differences in RF of the aerosol direct effect. As part of the AEROCOM phase 2 direct radiative forcing experiment, this study compares 3D concentration fields of black carbon from fossil fuel burning (BC) and sulphate (SO4) from a set of major global climate models. The participating models were run using a prescribed set of emissions of aerosol and aerosol precursors and the same meteorological year. We assume that model differences due to the aerosol vertical profile can be factored out from other differences such as aerosol physics, radiative transfer or ground albedo. We consequently analyse model RF variability using profiles of normalized RF (radiative forcing per unit mass, NDRF) calculated from a single model. This tool allows us to quantify the fraction of the intermodel variability due to differences in aerosol vertical profiles. We show that there are still significant differences between both modelled vertical density profiles, treatment of aerosol physics and other factors influencing the RF profiles.

  2. Refining transcriptional programs in kidney development by integration of deep RNA-sequencing and array-based spatial profiling

    PubMed Central

    2011-01-01

    Background The developing mouse kidney is currently the best-characterized model of organogenesis at a transcriptional level. Detailed spatial maps have been generated for gene expression profiling combined with systematic in situ screening. These studies, however, fall short of capturing the transcriptional complexity arising from each locus due to the limited scope of microarray-based technology, which is largely based on "gene-centric" models. Results To address this, the polyadenylated RNA and microRNA transcriptomes of the 15.5 dpc mouse kidney were profiled using strand-specific RNA-sequencing (RNA-Seq) to a depth sufficient to complement spatial maps from pre-existing microarray datasets. The transcriptional complexity of RNAs arising from mouse RefSeq loci was catalogued; including 3568 alternatively spliced transcripts and 532 uncharacterized alternate 3' UTRs. Antisense expressions for 60% of RefSeq genes was also detected including uncharacterized non-coding transcripts overlapping kidney progenitor markers, Six2 and Sall1, and were validated by section in situ hybridization. Analysis of genes known to be involved in kidney development, particularly during mesenchymal-to-epithelial transition, showed an enrichment of non-coding antisense transcripts extended along protein-coding RNAs. Conclusion The resulting resource further refines the transcriptomic cartography of kidney organogenesis by integrating deep RNA sequencing data with locus-based information from previously published expression atlases. The added resolution of RNA-Seq has provided the basis for a transition from classical gene-centric models of kidney development towards more accurate and detailed "transcript-centric" representations, which highlights the extent of transcriptional complexity of genes that direct complex development events. PMID:21888672

  3. Method development for impurity profiling in SFC: The selection of a dissimilar set of stationary phases.

    PubMed

    Galea, Charlene; Mangelings, Debby; Heyden, Yvan Vander

    2015-01-01

    Supercritical fluid chromatography (SFC) is drawing considerable interest as separation technique in the pharmaceutical industry. The technique is already well established in chiral separations both analytically and on a preparative scale. The use of SFC as a technique for drug impurity profiling is examined here. To define starting conditions in method development for drug impurity profiling, a set of dissimilar stationary phases is screened in parallel. The possibility to select a set of dissimilar columns using the retention factors (k-values) for a set of 64 drugs measured on 27 columns in SFC was examined. Experiments were carried out at a back-pressure of 150 bar and 25 °C with a mobile phase consisting of CO2 and methanol with 0.1% isopropylamine (5-40% over 10 min) at a flow rate of 3 mL/min. These k-values were then used to calculate correlation coefficients on the one hand and to perform a principal component analysis on the other. The Kennard and Stone algorithm, besides dendrograms and correlation-coefficient colour maps were used to select a set of 6 dissimilar stationary phases. The stationary phase characterization results from this study were compared to those from previous studies found in the literature. Retention mechanisms for compounds possessing different properties were also evaluated. The dissimilarity of the selected subset of 6 stationary phases was validated using mixtures of compounds with similar properties and structures, as one can expect in a drug impurity profile. PMID:25630237

  4. Phase diagram of a thin film of 3He confined within a 1.08 μm deep cavity

    NASA Astrophysics Data System (ADS)

    Zhelev, Nikolay; Sebastian, Abhilash; Smith, Eric; Parpia, Jeevak

    We describe measurements of superfluid 3He confined to a high-aspect ratio cavity within the head of a high quality factor torsion pendulum. Superfluid phase diagram for the confined thin film of fluid is predicted to be radically different compared to that of the bulk. In particular, at low pressures at the onset of the A-B transition, a ``stripe phase'' of alternating degenerate domains of B phase is predicted to occur. By tracking the torsion pendulum frequency and quality factor, we identify a well-defined superfluid transition for the fluid within the pendulum head. At lower temperatures, sharp transitions from the A phase to the B phase on cooling and a gradual transition from the B phase to the A phase on warming are observed. The values for the ratio of the cavity depth and the coherence length (D / ξ (T , P)) at the transitions match well the values of the transitions seen in the NMR measurements of 3He confined to a 700 nm deep cavity. At present, we do not see any evidence in our measurements that the ``stripe phase'' is realized at the A-B phase boundary. We acknowledge support from NSF grant: DMR 1202991.

  5. Experimental phase determination of the structure factor from Kossel line profile

    PubMed Central

    Faigel, G.; Bortel, G.; Tegze, M.

    2016-01-01

    Kossel lines are formed when radiation from point x-ray sources inside a single crystal are diffracted by the crystal itself. In principle, Kossel line patterns contain full information on the crystalline structure: phase and magnitude of the structure factors. The phase is coded into the profile of the lines. Although this was known for a long time, experimental realization has not been presented. In this work we demonstrate experimentally that phases can be directly determined from the profile of the Kossel lines. These measurements are interesting not only theoretically, but they would facilitate structure solution of samples within extreme conditions, such as high pressure, high and low temperatures, high magnetic fields and extremely short times. The parallel measurement of many diffraction lines on a stationary sample will allow a more efficient use of the new generation of x-ray sources the X-ray free electron lasers (XFELs). PMID:26965321

  6. Experimental phase determination of the structure factor from Kossel line profile.

    PubMed

    Faigel, G; Bortel, G; Tegze, M

    2016-01-01

    Kossel lines are formed when radiation from point x-ray sources inside a single crystal are diffracted by the crystal itself. In principle, Kossel line patterns contain full information on the crystalline structure: phase and magnitude of the structure factors. The phase is coded into the profile of the lines. Although this was known for a long time, experimental realization has not been presented. In this work we demonstrate experimentally that phases can be directly determined from the profile of the Kossel lines. These measurements are interesting not only theoretically, but they would facilitate structure solution of samples within extreme conditions, such as high pressure, high and low temperatures, high magnetic fields and extremely short times. The parallel measurement of many diffraction lines on a stationary sample will allow a more efficient use of the new generation of x-ray sources the X-ray free electron lasers (XFELs). PMID:26965321

  7. Frozen subduction in the Yangtze block: insights from the deep seismic profiling and gravity anomaly in east Sichuan fold belt

    NASA Astrophysics Data System (ADS)

    Xiong, Xiaosong; Gao, Rui; Wang, Haiyan; Zhang, Jisheng; Guo, Lianghui

    2016-04-01

    The Sichuan basin is the main part of the middle-upper Yangtze block, which has been experienced a long-term tectonic evolution since Archean. The Yangtze block was regarded as a stable block until the collision with the Cathaysia block in late Neoproterozoic. A new deep seismic reflection profile conducted in the eastern Sichuan fold belt (ESFB) discovered a serials of south-dipping reflectors shown from lower crust to the mantle imply a frozen subduction zone within the Yangtze block. In order to prove the speculation, we also obtain the middle-lower crustal gravity anomalies by removing the gravity anomalies induced by the sedimentary rocks and the mantle beneath the Moho, which shows the mid-lower crustal structure of the Sichuan basin can be divided into eastern and western parts. Combined with the geochronology and Aeromagnetic anomalies, we speculated the Yangtze block was amalgamated by the West Sichuan and East Sichuan blocks separated by the Huayin-Chongqing line. The frozen subduction zone subsequently shifted to a shear zone accommodated the lower crustal shortening when the decollement at the base of the Nanhua system functioned in the upper plate.

  8. Extraction of seawater-derived neodymium from different phases of deep sea sediments by selective leaching

    NASA Astrophysics Data System (ADS)

    Blaser, P.; Lippold, J. A.; Frank, N.; Gutjahr, M.; Böhm, E.

    2014-12-01

    In order to deduce reliable information about the interaction of the oceans with the climate system as a whole in the past, the reconstruction of water mass circulation is crucial. The analysis of seawater-derived neodymium isotopes (143Nd/144Nd, expressed as ɛNd) in marine sediments provides a unique proxy for deep water provenance in particular in the Atlantic [1]. The ɛNd signature and thus the mixing proportion of the local bottom water masses is archived in authigenic phases in the sediment. Obtaining seawater ɛNd from authigenic accretions bound to foraminiferal tests has lately become the preferred since most reliable method [2]. Attempts have also been made to extract the Nd-rich authigenic metal fraction by leaching it off the bulk sediment and thereby use this proxy with less effort, in the highest possible resolution and in sediments where foraminifera are not sufficiently present. However, often other sedimentary components are also leached in the process and contaminate the extracted Nd [3,4]. In this project several core-top and older sediments across the Atlantic have been leached in ten consecutive steps with either dilute buffered acetic acid or an acid-reductive solution. The leachates were analysed on their elemental and Nd isotope compositions, as well as rare earth element (REE) distributions. By graduating the total leaching procedure into smaller stages the results display which processes take place in the course of sediment leaching in the laboratory and which components of the sediment are most reactive. Thus, they help to better evaluate the quality of sediment leaches for ɛNd analysis. Clearly, organic calcite acts as a fast reacting buffer and at the point where its amount is sufficiently reduced the leaching of other components commences and the Nd concentration peaks. Corruption of the extracted ɛNd signal by non-authigenic sources in many cases occured early in the leaching sequence, indicating that only very cautious leaching

  9. Phase behavior of elastin-like synthetic recombinamers in deep eutectic solvents.

    PubMed

    Nardecchia, Stefania; Gutiérrez, María C; Ferrer, M Luisa; Alonso, Matilde; López, Isabel M; Rodríguez-Cabello, J Carlos; del Monte, Francisco

    2012-07-01

    Deep eutectic solvents promoted the stabilization of the collapsed state of elastin-like recombinamers - and the subsequent formation of aggregates - upon the loss of the structural water molecules involved in hydrophobic hydration. Cryo-etch scanning electron microscopy allowed the observation of these aggregates in neat deep eutectic solvents. The suppression of the lower critical solution temperature transition, observed by differential scanning calorimetry and dynamic light scattering, confirmed the presence of the elastin-like recombinamers in their collapsed state. Actually, the transition from the collapsed to the expanded state was suppressed even after moderate aqueous dilution - for water contents ranging from nil to ca. 45 wt % - and it was only recovered upon further addition of water - above 50 wt %. These features revealed the preferred stabilization of the collapsed state in not only neat deep eutectic solvents but also partially hydrated deep eutectic solvents. We consider that the capability to trigger the lower critical solution temperature transition by partial hydration of deep eutectic solvent may open interesting perspectives for nano(bio)technological applications of elastin-like recombinamers. PMID:22632070

  10. Pressure Profiles in Two-Phase Geothermal Wells: Comparison of Field Data and Model Calculations

    SciTech Connect

    Ambastha, A.K.; Gudmundsson, J.S.

    1986-01-21

    Increased confidence in the predictive power of two-phase correlations is a vital part of wellbore deliverability and deposition studies for geothermal wells. Previously, the Orkiszewski (1967) set of correlations has been recommended by many investigators to analyze geothermal wellbore performance. In this study, we use measured flowing pressure profile data from ten geothermal wells around the world, covering a wide range of flowrate, fluid enthalpy, wellhead pressure and well depth. We compare measured and calculated pressure profiles using the Orkiszewski (1967) correlations.

  11. Profile analysis of ventricle specimen based on a new phase measuring method

    NASA Astrophysics Data System (ADS)

    Cao, Yiping; Su, Xianyu; Chen, Wenjing; Xiang, Liqun; Zhang, Qichan; Liu, Yuankun

    2009-08-01

    The size and shape of ventricle are very important to analyze and diagnose pathology of human heart. So it is very necessary to measure the profile of ventricle. It is very difficult to measure the ventricle by vivisectional method for its unique function of heart, so the ventricle specimen is adopted to be measured. Three-dimensional (3D) automatic measurement methods are widely used in many fields. In Biology and Medicine society, it can be applicable for surgery, orthopedics, viscera disease analysis and diagnosis etc. Here a new method to measure the 3D surface of ventricle specimen is proposed. Although the traditional 3D measuing method with equal or stated phase-shifting step length possess excellent accuracy, they are much dependent on the consistency of these phase-shifting step lengths. In fact, this condition is very difficult to guarantee. which may lead to the incorrect wrapped phase and incorrect phase unwrapping in some regions, even the reconstructed object may be misshapen or anamorphic. In the proposed method, a novel improved three undecided step lengths phase-shifting algorithm with three unequal phase-shifting steps has been presented detailed and is applied to measure the profile of ventricle sucssesfully. Experiments show that the improved algorithm can not only effectively improve the measuring accuracy, but also branch out its application.

  12. The Application of Silicon Rich Nitride Films for Use as Deep-Ultraviolet Lithography Phase-Shifting Masks

    NASA Astrophysics Data System (ADS)

    Jiang, Zhong-Tao; Yamaguchi, Tomuo; Ohshimo, Kentaro; Aoyama, Mitsuru; Asinovsky, Leo

    1998-02-01

    Silicon rich nitride (SiRN) film prepared by plasma enhanced chemical vapor deposition (PECVD) for use as the phase-shifting mask for Deep-ultraviolet (UV) lithography has been developed. Optical properties and compositional characterizations of the SiRN films using Auger electron spectroscopy (AES) and spectroscopic ellipsometry (SE) combined with an empirical dielectric function (EDF), as well as phase-shifting mask simulation show that the SiRN is feasible for use in the application of single layer halftone phase-shifting mask (SLHTPSM) in the Deep-UV range. Optical constants of n ≈ 2.5 and k < 0.6 at 193 nm were realized by approaching the N/Si composition to the stoichiometric ratio of Si3N4. The deposition conditions for the films having the transmittance of 5 - 10% with a 180° phase shift at 193 nm (ArF) have been determined. Short wavelength extrapolation by EDF best-fit parameters based on a proper film-stack model provides a potential method to characterize the optical properties of amorphous SiRN down to about 190 nm, which is outside the range of most commercial SE's.

  13. Directivity of a Sparse Array in the Presence of Atmospheric-Induced Phase Fluctuations for Deep Space Communications

    NASA Technical Reports Server (NTRS)

    Nessel, James A.; Acosta, Robert J.

    2010-01-01

    Widely distributed (sparse) ground-based arrays have been utilized for decades in the radio science community for imaging celestial objects, but have only recently become an option for deep space communications applications with the advent of the proposed Next Generation Deep Space Network (DSN) array. But whereas in astronomical imaging, observations (receive-mode only) are made on the order of minutes to hours and atmospheric-induced aberrations can be mostly corrected for in post-processing, communications applications require transmit capabilities and real-time corrections over time scales as short as fractions of a second. This presents an unavoidable problem with the use of sparse arrays for deep space communications at Ka-band which has yet to be successfully resolved, particularly for uplink arraying. In this paper, an analysis of the performance of a sparse antenna array, in terms of its directivity, is performed to derive a closed form solution to the expected array loss in the presence of atmospheric-induced phase fluctuations. The theoretical derivation for array directivity degradation is validated with interferometric measurements for a two-element array taken at Goldstone, California. With the validity of the model established, an arbitrary 27-element array geometry is defined at Goldstone, California, to ascertain its performance in the presence of phase fluctuations. It is concluded that a combination of compact array geometry and atmospheric compensation is necessary to ensure high levels of availability.

  14. 30 CFR 203.43 - To which production do I apply the RSV earned from qualified deep wells or qualified phase 1...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false To which production do I apply the RSV earned from qualified deep wells or qualified phase 1 ultra-deep wells on my lease? 203.43 Section 203.43 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN...

  15. 30 CFR 203.40 - Which leases are eligible for royalty relief as a result of drilling a deep well or a phase 1...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false Which leases are eligible for royalty relief as a result of drilling a deep well or a phase 1 ultra-deep well? 203.40 Section 203.40 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN ROYALTY RATES...

  16. Deformation mechanisms in Phase D to 45 GPa and implications for the seismic anisotropy in deep subducted slabs

    NASA Astrophysics Data System (ADS)

    Rosa, A. D.; Sanchez-Valle, C.; Nisr, C.; Bollinger, C.; Evans, S.; Merkel, S.

    2010-12-01

    Petrologic investigations have shown that phase D, MgSi2H2O6, may be the dominant hydrous phase along a cold slab geotherm, accounting for more than 40 vol.% of very hydrous subducted peridotites between 700 to 1300 km depth [Iwamori, 2004]. Such large volume fractions of phase D are likely to have an important impact on seismic properties in subduction zones and has been appealed to explain a number of seismic observation in subducted plates including velocity heterogeneity and high shear-wave splitting (VSH > VSV) [Chen and Brudzinki, 2003; Mainprice et al. 2007, Laurence & Wysession, 2006]. The layered structure of Phase D behaves highly anisotropic under compression and is likely to align in a non-hydrostatic stress field, being a strong candidate to contribute to the seismic shear wave splitting observed in deep subducted slabs. In order to interpret these seismic observations in terms of mineralogy, deformation state and degree of hydration, precisely determined rheology and elasticity data of candidate phases at relevant pressure and temperature conditions are needed. In this contribution we present results of the plastic deformation behavior of Phase D up to 45 GPa. Experiments were conducted using synchrotron radial x-ray diffraction at ESRF ID09A and a panoramic diamond anvil cell as deformation apparatus. Phase D samples with three different compositions, including pure Mg-Phase D, Fe-bearing and Fe-Al-bearing Phase D, were investigated to constrain the effect of cation substitution on the deformation mechanism. The samples were synthesized at 19 GPa and about 1100 °C in a multi-anvil press at ETH Zurich and confirmed to be Phase D by single-crystal x-ray diffraction and Raman spectroscopy. Recovered crystals were reduced to fine-grained powders and loaded in x-ray transparent boron gaskets for the experiments. Upon compression, the development of strong textures in the samples was observed from the intensity variations of Debye rings. The observed slip

  17. Effect of osmotic pressure in the solvent extraction phase on BSA release profile from PLGA microspheres.

    PubMed

    Jiang, Ge; Thanoo, B C; DeLuca, Patrick P

    2002-11-01

    This study investigated the influence of osmotic pressure in the organic solvent extraction phase on release profile of bovine serum albumin (BSA) from poly(lactide-co-glycolide) (PLGA) microspheres. BSA-loaded PLGA microspheres with a target load of 10% were prepared by a double emulsion phase separation method. All the microsphere batches were fabricated in the same conditions except that in the organic solvent (CH2Cl2) evaporation step. Different concentrations of NaCl (0, 1.8, and 3.6%) or sucrose (20%) were used to generate a range of osmotic pressures in the extraction aqueous phase. These microspheres were characterized for incorporation efficiency, surface and internal morphology, particle size, protein stability, and in vitro release. The microspheres were spherical with particle size ranging from 16.8 to 27.8 microns. Higher osmotic pressure resulted in a denser internal structure although similar nonporous surface morphology was observed with all batches. No significant difference in encapsulation efficiency existed from batch to batch (87-94%). Sodium dodecyl sulfate-polyamide gel electrophoresis showed that BSA integrity was well retained. The release profile of the batch prepared with only water as the continuous (solvent extraction) phase exhibited a 79% burst release in the first 24 hr followed by a plateau and then a little release after 21 days. In the presence of NaCl or sucrose, the burst effect significantly decreased with increase in osmotic pressure in the extraction aqueous phase, which was then followed by sustained release for 35 days. A mass balance was made when the release terminated. Therefore, in the organic solvent extraction and evaporation step, increasing the osmotic pressure in the aqueous phase both reduced the burst release from the microspheres and improved the subsequent sustained release profile. PMID:12503521

  18. Phase II monitoring of auto-correlated linear profiles using linear mixed model

    NASA Astrophysics Data System (ADS)

    Narvand, A.; Soleimani, P.; Raissi, Sadigh

    2013-05-01

    In many circumstances, the quality of a process or product is best characterized by a given mathematical function between a response variable and one or more explanatory variables that is typically referred to as profile. There are some investigations to monitor auto-correlated linear and nonlinear profiles in recent years. In the present paper, we use the linear mixed models to account autocorrelation within observations which is gathered on phase II of the monitoring process. We undertake that the structure of correlated linear profiles simultaneously has both random and fixed effects. The work enhanced a Hotelling's T 2 statistic, a multivariate exponential weighted moving average (MEWMA), and a multivariate cumulative sum (MCUSUM) control charts to monitor process. We also compared their performances, in terms of average run length criterion, and designated that the proposed control charts schemes could effectively act in detecting shifts in process parameters. Finally, the results are applied on a real case study in an agricultural field.

  19. Pulse-combustion deep fat fryer, Phase 2. Final report, December 1983-December 1985

    SciTech Connect

    Farnsworth, C.A.

    1986-01-01

    A pulse-combustion burner system was developed that met both efficiency and cooking-performance goals. Work involved modifications to the pulse-combustion deep fat fryer to reduce the sound level, eliminate vibrations, and ensure reliable ignition. Field testing in a major fast-food restaurant chain was initiated, demonstrating that the pulse fryer is reliable and acceptable.

  20. Basin formation and inversion of the back-arc, Niigata basin, central Japan: New insight from deep seismic profiling

    NASA Astrophysics Data System (ADS)

    Sato, Hiroshi; Abe, Susumu; Kawai, Nobuo; Saito, Hideo; Kato, Naoko; Shiraishi, Kazuya; Ishiyama, Tatsuya; Fukasawa, Hikaru; Inaba, Mitsuru

    2010-05-01

    Associated with the opening of the Japan Sea, rift-basins have been developed along the Japan Sea coast of northern Honshu. The Niigata basin, central Japan, is one of the such basins and filled by thick (< 8 km) Neogene sediments. By subsequent convergence since the Pliocene, an arc-parallel fold-and-thrust-belt has been developed along the Miocene rift-basins. In this belt devastative earthquakes, such as 1964 Niigata (M7.4), 2004 Chuetsu (M6.8) and 2007 Chuetsu-oki (M6.8) earthquakes, occurred by reverse faulting. Due to thick Neogene sediments, relationship between active faults/folds at near the surface and deep-sited seismogenic source faults is poorly understood. To reveal the crustal structure, in particular geometry of source faults, onshore-offshore integrated deep seismic profiling was undertaken along the two seismic lines in 2008 and 2009. The 2009 Aizu-Sado seismic line is a 135-km-long, onshore-offshore seismic line across Niigata basin and Sado island, which is located in the eastern part of Japan Sea. The 2008 Sanjo-Yahiko seismic line (Sato et al., 2009) is located 20 km south of the seismic line and trending parallel to it. The seismic source was air-gun (3020 cu. inch), four vibroseis trucks and explosives (< 200kg). Along the Sado strait, seismic data was acquired using two-ships to make large offset shot gather. Seismic signals were recorded by ocean bottom cables, cable-connected-recording system and offline recorders, forming a maximum 2400 channels receiver array. The basin fill consists of early to middle Miocene volcaniclastic rocks and overlying Neogene sedimentary rocks showing upward coarsening facies deposited under bathyal to fluvial environment. Main features of basin development, such as early Miocene normal faulting, associated with the formation of Japan Sea, and shortening deformation since Pliocene, are well demonstrated on the seismic sections. Particularly, boundary between pre-Tertiary meta-sedimentary rocks and Miocene

  1. Development of a High Performance, Low Profile Translation Table with Wire Feedthrough for a Deep Space CubeSat

    NASA Technical Reports Server (NTRS)

    Few, Alex

    2016-01-01

    NEAScout, a 6U cubesat and secondary payload on NASA's EM-1, will use an 85 sq m solar sail to travel to a near-earth asteroid at about 1 Astronomical Unit (about 1.5 x 10(exp 8) km) for observation and reconnaissance1. A combination of reaction wheels, reaction control system, and a slow rotisserie roll about the solar sail's normal axis were expected to handle attitude control and adjust for imperfections in the deployed sail during the 2.5-year mission. As the design for NEAScout matured, one of the critical design parameters, the offset in the center of mass and center of pressure (CP/CM offset), proved to be sub-optimal. After significant mission and control analysis, the CP/CM offset was accommodated by the addition of a new subsystem to NEAScout. This system, called the Active Mass Translator (AMT), would reside near the geometric center of NEAScout and adjust the CM by moving one portion of the flight system relative to the other. The AMT was given limited design space - 17 mm of the vehicle's assembly height-and was required to generate +/-8 cm by +/-2 cm translation to sub-millimeter accuracy. Furthermore, the design must accommodate a large wire bundle of small gage, single strand wire and coax cables fed through the center of the mechanism. The bend radius, bend resistance, and the exposure to deep space environment complicates the AMT design and operation and necessitated a unique design to mitigate risks of wire bundle damage, binding, and cold-welding during operation. This paper will outline the design constraints for the AMT, discuss the methods and reasoning for design, and identify the lessons learned through the designing, breadboarding and testing for the low-profile translation stages with wire feedthrough capability.

  2. Sensory, psychological, and metabolic dysfunction in HIV-associated peripheral neuropathy: A cross-sectional deep profiling study

    PubMed Central

    Phillips, Tudor J.C.; Brown, Matthew; Ramirez, Juan D.; Perkins, James; Woldeamanuel, Yohannes W.; Williams, Amanda C. de C.; Orengo, Christine; Bennett, David L.H.; Bodi, Istvan; Cox, Sarah; Maier, Christoph; Krumova, Elena K.; Rice, Andrew S.C.

    2014-01-01

    HIV-associated sensory neuropathy (HIV-SN) is a frequent complication of HIV infection and a major source of morbidity. A cross-sectional deep profiling study examining HIV-SN was conducted in people living with HIV in a high resource setting using a battery of measures which included the following: parameters of pain and sensory symptoms (7 day pain diary, Neuropathic Pain Symptom Inventory [NPSI] and Brief Pain Inventory [BPI]), sensory innervation (structured neurological examination, quantitative sensory testing [QST] and intraepidermal nerve fibre density [IENFD]), psychological state (Pain Anxiety Symptoms Scale-20 [PASS-20], Depression Anxiety and Positive Outlook Scale [DAPOS], and Pain Catastrophizing Scale [PCS], insomnia (Insomnia Severity Index [ISI]), and quality of life (Short Form (36) Health Survey [SF-36]). The diagnostic utility of the Brief Peripheral Neuropathy Screen (BPNS), Utah Early Neuropathy Scale (UENS), and Toronto Clinical Scoring System (TCSS) were evaluated. Thirty-six healthy volunteers and 66 HIV infected participants were recruited. A novel triumvirate case definition for HIV-SN was used that required 2 out of 3 of the following: 2 or more abnormal QST findings, reduced IENFD, and signs of a peripheral neuropathy on a structured neurological examination. Of those with HIV, 42% fulfilled the case definition for HIV-SN (n = 28), of whom 75% (n = 21) reported pain. The most frequent QST abnormalities in HIV-SN were loss of function in mechanical and vibration detection. Structured clinical examination was superior to QST or IENFD in HIV-SN diagnosis. HIV-SN participants had higher plasma triglyceride, concentrations depression, anxiety and catastrophizing scores, and prevalence of insomnia than HIV participants without HIV-SN. PMID:24973717

  3. Online, efficient and precision laser profiling of bronze-bonded diamond grinding wheels based on a single-layer deep-cutting intermittent feeding method

    NASA Astrophysics Data System (ADS)

    Deng, Hui; Chen, Genyu; He, Jie; Zhou, Cong; Du, Han; Wang, Yanyi

    2016-06-01

    In this study, an online, efficient and precision laser profiling approach that is based on a single-layer deep-cutting intermittent feeding method is described. The effects of the laser cutting depth and the track-overlap ratio of the laser cutting on the efficiency, precision and quality of laser profiling were investigated. Experiments on the online profiling of bronze-bonded diamond grinding wheels were performed using a pulsed fiber laser. The results demonstrate that an increase in the laser cutting depth caused an increase in the material removal efficiency during the laser profiling process. However, the maximum laser profiling efficiency was only achieved when the laser cutting depth was equivalent to the initial surface contour error of the grinding wheel. In addition, the selection of relatively high track-overlap ratios of laser cutting for the profiling of grinding wheels was beneficial with respect to the increase in the precision of laser profiling, whereas the efficiency and quality of the laser profiling were not affected by the change in the track-overlap ratio. After optimized process parameters were employed for online laser profiling, the circular run-out error and the parallelism error of the grinding wheel surface decreased from 83.1 μm and 324.6 μm to 11.3 μm and 3.5 μm, respectively. The surface contour precision of the grinding wheel significantly improved. The highest surface contour precision for grinding wheels of the same type that can be theoretically achieved after laser profiling is completely dependent on the peak power density of the laser. The higher the laser peak power density is, the higher the surface contour precision of the grinding wheel after profiling.

  4. CosmoTransitions: Computing cosmological phase transition temperatures and bubble profiles with multiple fields

    NASA Astrophysics Data System (ADS)

    Wainwright, Carroll L.

    2012-09-01

    I present a numerical package (CosmoTransitions) for analyzing finite-temperature cosmological phase transitions driven by single or multiple scalar fields. The package analyzes the different vacua of a theory to determine their critical temperatures (where the vacuum energy levels are degenerate), their supercooling temperatures, and the bubble wall profiles which separate the phases and describe their tunneling dynamics. I introduce a new method of path deformation to find the profiles of both thin- and thick-walled bubbles. CosmoTransitions is freely available for public use.Program summaryProgram Title: CosmoTransitionsCatalogue identifier: AEML_v1_0Program summary URL: http://cpc.cs.qub.ac.uk/summaries/AEML_v1_0.htmlProgram obtainable from: CPC Program Library, Queen's University, Belfast, N. IrelandLicensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.htmlNo. of lines in distributed program, including test data, etc.: 8775No. of bytes in distributed program, including test data, etc.: 621096Distribution format: tar.gzProgramming language: Python.Computer: Developed on a 2009 MacBook Pro. No computer-specific optimization was performed.Operating system: Designed and tested on Mac OS X 10.6.8. Compatible with any OS with Python installed.RAM: Approximately 50 MB, mostly for loading plotting packages.Classification: 1.9, 11.1.External routines: SciPy, NumPy, matplotLibNature of problem: I describe a program to analyze early-Universe finite-temperature phase transitions with multiple scalar fields. The goal is to analyze the phase structure of an input theory, determine the amount of supercooling at each phase transition, and find the bubble-wall profiles of the nucleated bubbles that drive the transitions.Solution method: To find the bubble-wall profile, the program assumes that tunneling happens along a fixed path in field space. This reduces the equations of motion to one dimension, which can then be solved using the overshoot

  5. Internal friction measurements of phase transformations during the process of deep cryogenic treatment of a tool steel

    NASA Astrophysics Data System (ADS)

    Li, Shaohong; Min, Na; Li, Junwan; Wu, Xiaochun

    2013-10-01

    This paper reports internal friction measurements in die steel with the aim of clarifying phase transformation mechanisms during deep cryogenic treatment (DCT). Internal friction peaks appeared in the quenched sample and the DCT treated material during the process of cooling down to -160 °C from room temperature (20 °C) and then heating up to room temperature for multiple cycles in high vacuum. However, internal friction peaks do not appear in annealed material. It is concluded that the internal friction peaks are attributed to stress-induced martensite formation based on thermal hysteresis phenomena. That is, retained austenite is mostly transformed into martensite during the DCT.

  6. Utilization of deep eutectic solvents as novel mobile phase additives for improving the separation of bioactive quaternary alkaloids.

    PubMed

    Tan, Ting; Zhang, Mingliang; Wan, Yiqun; Qiu, Hongdeng

    2016-03-01

    Deep eutectic solvents (DESs) were used as novel mobile phase additives to improve chromatographic separation of four quaternary alkaloids including coptisine chloride, sanguinarine, berberine chloride and chelerythrine on a C18 column. DESs as a new class of ionic liquids are renewably sourced, environmentally benign, low cost and easy to prepare. Seven DESs were obtained by mixing different hydrogen acceptors and hydrogen-bond donors. The effects of organic solvents, the concentration of DESs, the types of DESs and the pH values of the buffer solution on the separation of the analytes were investigated. The composition of acetonitrile and 1.0% deep eutectic solvents aqueous solution (pH 3.3, adjusted with hydrochloric acid) in a 32:68 (v/v) ratio was used as optimized mobile phase, with which four quaternary alkaloids were well separated. When a small amount of DESs was added in the mobile phase for the separation of alkaloids on the C18 column, noticeable improvements were distinctly observed such as decreasing peak tailing and improving resolution. The separation mechanism mediated by DESs as mobile phase additives can be attributed to combined effect of both hydrogen acceptors and hydrogen-bond donors. For example, choline chloride can effectively cover the residual silanols on silica surface and ethylene glycol can reduce the retention time of analytes. The proposed method has been applied to determine BerbC in Lanqin Chinese herbal oral solution and BerbC tablet. Utilization of DESs in mobile phase can efficiently improve separation and selectivity of analytes from complex samples. PMID:26717817

  7. Saturation Profiles from Lab-scale Permittivity Measurements and 2-Phase Flow Models.

    NASA Astrophysics Data System (ADS)

    Gorriti, A. G.

    2005-05-01

    sample, we obtain the permittivity profile. The permittivity is mainly determined by the saturation, then, the permittivity profile within the sample is equivalent to the saturation profile. One can be obtained from the other using the Complex Refractive Index Model (CRIM). The saturation profile can also be obtained with traditional 2-phase flow modelling. Preliminary results show that the saturation profile obtained from the permittivity is more close to reality than the one inferred from 2-phase flow modelling.

  8. Altered Circadian Rhythm and Metabolic Gene Profile in Rats Subjected to Advanced Light Phase Shifts

    PubMed Central

    Herrero, Laura; Valcarcel, Lorea; da Silva, Crhistiane Andressa; Albert, Nerea; Diez-Noguera, Antoni; Cambras, Trinitat; Serra, Dolors

    2015-01-01

    The circadian clock regulates metabolic homeostasis and its disruption predisposes to obesity and other metabolic diseases. However, the effect of phase shifts on metabolism is not completely understood. We examined whether alterations in the circadian rhythm caused by phase shifts induce metabolic changes in crucial genes that would predispose to obesity. Three-month-old rats were maintained on a standard diet under lighting conditions with chronic phase shifts consisting of advances, delays or advances plus delays. Serum leptin, insulin and glucose levels decreased only in rats subjected to advances. The expression of the clock gene Bmal 1 increased in the hypothalamus, white adipose tissue (WAT), brown adipose tissue (BAT) and liver of the advanced group compared to control rats. The advanced group showed an increase in hypothalamic AgRP and NPY mRNA, and their lipid metabolism gene profile was altered in liver, WAT and BAT. WAT showed an increase in inflammation and ER stress and brown adipocytes suffered a brown-to-white transformation and decreased UCP-1 expression. Our results indicate that chronic phase advances lead to significant changes in neuropeptides, lipid metabolism, inflammation and ER stress gene profile in metabolically relevant tissues such as the hypothalamus, liver, WAT and BAT. This highlights a link between alteration of the circadian rhythm and metabolism at the transcriptional level. PMID:25837425

  9. Origin and annealing of deep-level defects in GaNAs grown by metalorganic vapor phase epitaxy

    NASA Astrophysics Data System (ADS)

    Gelczuk, Ł.; Stokowski, H.; Dąbrowska-Szata, M.; Kudrawiec, R.

    2016-05-01

    Deep-level defects were investigated by deep level transient spectroscopy on the as-grown and annealed GaNAs layers of various nitrogen (N) contents. The unintentionally doped (uid) GaNAs layers were grown by metalorganic vapor phase epitaxy with N = 1.4%, 2.0%, 2.2%, and 2.4% on GaAs substrate. The possible origin and evolution of the deep-level defects upon annealing were analyzed with the use of the GaNAs band gap diagram concept [Kudrawiec et al., Appl. Phys. Lett. 101, 082109 (2012)], which assumes that the activation energy of donor traps decreases with N-related downward shift of the conduction band. On the basis of this diagram and in comparison with previous results, the N-related traps were associated with (N-As)As or (N-N)As split interstitials. It was also proposed that one of the electron traps and the hole trap, lying at the same level position in the bandgap of the annealed uid-GaNAs layers, can both act as one generation-recombination center partially responsible for poor optical properties of this alloy.

  10. A surface profile reconstruction system using sinusoidal phase-modulating interferometry and fiber-optic fringe projection

    NASA Astrophysics Data System (ADS)

    En, Bo; Fa-jie, Duan; Chang-rong, Lv; Fan, Feng; Xiao, Fu

    2014-06-01

    A fiber-optic sinusoidal phase modulating (SPM) interferometer for surface profile reconstruction is presented. Sinusoidal phase modulation is created by modulating the drive voltage of the piezoelectric transducer. The surface profile is constructed basing on fringe projection. Fringe patterns are vulnerable to external disturbances such as temperature fluctuation and mechanical vibration, which cause phase drift and decrease measuring accuracy. We build a closed-loop feedback phase compensation system, the bias value of external disturbances superimposed on fringe patterns can be reduced to about 50 mrad, and the phase stability for interference fringes is less than 5.76 mrad. By measuring the surface profile of a paper plate for two times, the repeatability is estimated to be about 11 nm, and is equivalent to be about λ/69. For a plane with 100 × 100 points, a single measurement takes less than 140 ms, and the feasibility for real-time profile measurement with high accuracy has been verified.

  11. Automated surface profile measurement of diamond grid disk by phase-shifted shadow Moiré

    NASA Astrophysics Data System (ADS)

    Chen, Terry Yuan-Fang; Lin, Jie

    2014-06-01

    Diamond grid disk dresser is frequently employed to remove the accumulated debris lest the polishing surface glazes. The surface warpage of diamond grid disk must be small enough to assure the flatness of polished wafers during chemical mechanical planarization process. In this study, phase-shifted shadow moiré method was employed to measure the surface profile of diamond grid disk. To eliminate erroneous bright or black spots caused by the diamond grids, a new approach is proposed by automatically selecting a proper threshold value from the differentiated image resulting from the addition of four phase-shifted images. According to the largest size of erroneous spot, the size of a structuring element is determined for morphology filtering. Thereafter the phase can be calculated and unwrapped correctly. Test of the method on a diamond grid disk is demonstrated and discussed.

  12. Advances in Scanning Reflectarray Antennas Based on Ferroelectric Thin Film Phase Shifters for Deep Space Communications

    NASA Technical Reports Server (NTRS)

    Romanofsky, Robert R.

    2007-01-01

    Though there are a few examples of scanning phased array antennas that have flown successfully in space, the quest for low-cost, high-efficiency, large aperture microwave phased arrays continues. Fixed and mobile applications that may be part of a heterogeneous exploration communication architecture will benefit from the agile (rapid) beam steering and graceful degradation afforded by phased array antennas. The reflectarray promises greater efficiency and economy compared to directly-radiating varieties. Implementing a practical scanning version has proven elusive. The ferroelectric reflectarray, under development and described herein, involves phase shifters based on coupled microstrip patterned on Ba(x)Sr(1-x)TiO3 films, that were laser ablated onto LaAlO3 substrates. These devices outperform their semiconductor counterparts from X- through and K-band frequencies. There are special issues associated with the implementation of a scanning reflectarray antenna, especially one realized with thin film ferroelectric phase shifters. This paper will discuss these issues which include: relevance of phase shifter loss; modulo 2(pi) effects and phase shifter transient effects on bit error rate; scattering from the ground plane; presentation of a novel hybrid ferroelectric-semiconductor phase shifter; and the effect of mild radiation exposure on phase shifter performance.

  13. Thermodynamic phase profiles of optically thin midlatitude cloud and their relation to temperature

    SciTech Connect

    Naud, C. M.; Del Genio, Anthony D.; Haeffelin, M.; Morille, Y.; Noel, V.; Dupont, Jean-Charles; Turner, David D.; Lo, Chaomei; Comstock, Jennifer M.

    2010-06-03

    Winter cloud phase and temperature profiles derived from ground-based lidar depolarization and radiosonde measurements are analyzed for two midlatitude locations: the United States Atmospheric Radiation Measurement Program Southern Great Plains (SGP) site and the Site Instrumental de Recherche par Télédétection Atmosphérique (SIRTA) in France. Because lidars are attenuated in optically thick clouds, the dataset only includes optically thin clouds (optical thickness < 3). At SGP, 57% of the clouds observed with the lidar in the temperature range 233-273 K are either completely liquid or completely glaciated, while at SIRTA only 42% of the observed clouds are single phase, based on a depolarization ratio threshold of 11% for differentiating liquid from ice. Most optically thin mixed phase clouds show an ice layer at cloud top, and clouds with liquid at cloud top are less frequent. The relationship between ice phase occurrence and temperature only slightly changes between cloud base and top. At both sites liquid is more prevalent at colder temperatures than has been found previously in aircraft flights through frontal clouds of greater optical thicknesses. Liquid in clouds persists to colder temperatures at SGP than SIRTA. This information on the average temperatures of mixed phase clouds at both locations complements earlier passive satellite remote sensing measurements that sample cloud phase near cloud top and for a wider range of cloud optical thicknesses.

  14. Kinetics of AlGaN metal–organic vapor phase epitaxy for deep-UV applications

    NASA Astrophysics Data System (ADS)

    Lobanova, Anna; Yakovlev, Eugene; Jeschke, Joerg; Knauer, Arne; Weyers, Markus

    2016-05-01

    Al x Ga1‑ x N layers with high aluminum content of x ∼ 0.68–0.73 were grown in an 11 × 2-in. AIX 2400 G3 HT planetary reactor by metal–organic vapor phase epitaxy. Growth trends are analyzed by reaction-transport modeling in a wide range of growth conditions. Gas-phase nucleation resulting in both Al and Ga consumption into nanoparticles is a major mechanism affecting the growth efficiencies of AlN and GaN. Process windows suitable to grow multiple quantum wells (MQWs) for deep UV applications are found for a range of pressures, temperatures, and V/III ratios.

  15. Common complications of deep lamellar keratoplasty in the early phase of the learning curve

    PubMed Central

    Hosny, Mohamed

    2011-01-01

    Purpose: To evaluate and record the common complications that face surgeons when they perform their first few series of deep lamellar keratoplasty and measures to avoid these. Setting: Dar El Oyoun Hospital, Cairo, Egypt. Methods: Retrospective study of the first 40 eyes of 40 patients carried out by two corneal surgeons working in the same center. All patients were planned to undergo a deep anterior lamellar keratoplasty using the big bubble technique. Twelve patients suffered from keratoconus while 28 patients had anterior corneal pathologies. Recorded complications were classified as either intraoperative or postoperative. Results: Perforation of Descemet’s membrane was the most common intraoperative complication. It occurred in nine eyes (22.5%): five eyes (12.5%) had microperforations while four eyes (10%) had macroperforations, three eyes (7.5%) had central perforations, and six eyes (15%) had peripheral perforations. Other complications included incomplete separation of Descemet’s membrane and remnants of peripheral stromal tissue. Postoperative complications included double anterior chamber which occurred in four eyes (10%) and Descemet’s membrane corrugations. Postoperative astigmatism ranged from 1.25 to 4.5 diopters with a mean of 2.86 diopters in the whole series, but in the six cases with identified residual stroma in the periphery of the host bed, the astigmatism ranged from 2.75 to 4.5 diopters with a mean of 3.62 diopters. Conclusion: Deep lamellar keratoplasty is sensitive to procedural details. Learning the common complications and how to avoid them helps novice surgeons to learn the procedure faster. PMID:21750612

  16. Metabolic profiles of prokaryotic and eukaryotic communities in deep-sea sponge Lamellomorpha sp. indicated by metagenomics

    NASA Astrophysics Data System (ADS)

    Li, Zhi-Yong; Wang, Yue-Zhu; He, Li-Ming; Zheng, Hua-Jun

    2014-01-01

    The whole metabolism of a sponge holobiont and the respective contributions of prokaryotic and eukaryotic symbionts and their associations with the sponge host remain largely unclear. Meanwhile, compared with shallow water sponges, deep-sea sponges are rarely understood. Here we report the metagenomic exploration of deep-sea sponge Lamellomorpha sp. at the whole community level. Metagenomic data showed phylogenetically diverse prokaryotes and eukaryotes in Lamellomorpha sp.. MEGAN and gene enrichment analyses indicated different metabolic potentials of prokaryotic symbionts from eukaryotic symbionts, especially in nitrogen and carbon metabolisms, and their molecular interactions with the sponge host. These results supported the hypothesis that prokaryotic and eukaryotic symbionts have different ecological roles and relationships with sponge host. Moreover, vigorous denitrification, and CO2 fixation by chemoautotrophic prokaryotes were suggested for this deep-sea sponge. The study provided novel insights into the respective potentials of prokaryotic and eukaryotic symbionts and their associations with deep-sea sponge Lamellomorpha sp..

  17. Experimental confirmation of calculated phases and electron density profile for wet native collagen.

    PubMed Central

    Stinson, R H; Bartlett, M W; Kurg, T; Sweeny, P R; Hendricks, R W

    1979-01-01

    An experimental procedure is developed to phase the reflections obtained in x-ray diffraction investigations of collagen in native wet tendons. Phosphotungstic acid was used for isomorphous addition in phase determination and was located by electron microscopy. Structure factors (with phases) were obtained from the electron microscopy data for the heavy metal. Structure-factor magnitudes for collagen with and without the heavy metal were obtained from the x-ray diffraction data. The first 10 orders were investigated. Standard Argand diagrams provided two solutions for each of these, except the weak sixth order. In each case, one of the two possible solutions agrees well with the phases proposed on theoretical grounds by Hulmes et al. The present results suggest that their other proposed phases are probably correct. An electron density profile along the unit cell of the fibril is presented that shows a distinct step, as expected on the basis of the hole-overlap model. The overlap region is 48% of the length of the unit cell. Images FIGURE 2 PMID:262416

  18. Deep soil carbon dynamics are driven more by soil type than by climate: a worldwide meta-analysis of radiocarbon profiles.

    PubMed

    Mathieu, Jordane A; Hatté, Christine; Balesdent, Jérôme; Parent, Éric

    2015-11-01

    The response of soil carbon dynamics to climate and land-use change will affect both the future climate and the quality of ecosystems. Deep soil carbon (>20 cm) is the primary component of the soil carbon pool, but the dynamics of deep soil carbon remain poorly understood. Therefore, radiocarbon activity (Δ14C), which is a function of the age of carbon, may help to understand the rates of soil carbon biodegradation and stabilization. We analyzed the published 14C contents in 122 profiles of mineral soil that were well distributed in most of the large world biomes, except for the boreal zone. With a multivariate extension of a linear mixed-effects model whose inference was based on the parallel combination of two algorithms, the expectation-maximization (EM) and the Metropolis-Hasting algorithms, we expressed soil Δ14C profiles as a four-parameter function of depth. The four-parameter model produced insightful predictions of soil Δ14C as dependent on depth, soil type, climate, vegetation, land-use and date of sampling (R2=0.68). Further analysis with the model showed that the age of topsoil carbon was primarily affected by climate and cultivation. By contrast, the age of deep soil carbon was affected more by soil taxa than by climate and thus illustrated the strong dependence of soil carbon dynamics on other pedologic traits such as clay content and mineralogy. PMID:26119088

  19. Measuring two-phase particle flux with a multi-frequency acoustic Doppler profiler.

    PubMed

    Wilson, Gregory W; Hay, Alex E

    2015-12-01

    A methodology is developed and tested for simultaneously extracting time-resolved one-dimensional profiles of the mass-concentration and velocity of two different particle types in a mixed suspension, using a multi-frequency pulse-to-pulse coherent Doppler instrument. The technique involves inversion of a model for frequency-dependent acoustic backscatter amplitude and phase. Results are presented from a laboratory settling column experiment, measuring a mixture of polystyrene beads (slowly-settling, strongly-scattering) and glass beads (quickly-settling, weakly-scattering) in a vertical pipe section. PMID:26723335

  20. Viscous singular shock profiles for a system of conservation laws modeling two-phase flow

    NASA Astrophysics Data System (ADS)

    Hsu, Ting-Hao

    2016-08-01

    This paper is concerned with singular shocks for a system of conservation laws via the Dafermos regularization ut + f(u)x = ɛtuxx. For a system modeling incompressible two-phase fluid flow, the existence of viscous profiles is proved using Geometric Singular Perturbation Theory. The weak convergence and the growth rate of the viscous solution are also derived; the weak limit is the sum of a piecewise constant function and a δ-measure supported on a shock line, and the maximum value of the viscous solution is of order exp ⁡ (1 / ɛ).

  1. Profiles.

    ERIC Educational Resources Information Center

    School Arts, 1979

    1979-01-01

    Profiles seven Black, Native American, and Chicano artists and art teachers: Hale A. Woodruff, Allan Houser, Luis Jimenez, Betrand D. Phillips, James E. Pate, I, and Fernando Navarro. This article is part of a theme issue on multicultural art. (SJL)

  2. A Study of Phased Array Antennas for NASA's Deep Space Network

    NASA Technical Reports Server (NTRS)

    Jamnejad, Vahraz; Huang, John; Cesarone, Robert J.

    2001-01-01

    In this paper we briefly discuss various options but focus on the feasibility of the phased arrays as a viable option for this application. Of particular concern and consideration will be the cost, reliability, and performance compared to the present 70-meter antenna system, particularly the gain/noise temperature levels in the receive mode. Many alternative phased arrays including planar horizontal arrays, hybrid mechanically/electronically steered arrays, phased array of mechanically steered reflectors, multi-faceted planar arrays, phased array-fed lens antennas, and planar reflect-arrays are compared and their viability is assessed. Although they have many advantages including higher reliability, near-instantaneous beam switching or steering capability, the cost of such arrays is presently prohibitive and it is concluded that the only viable array options at the present are the arrays of a few or many small reflectors. The active planar phased arrays, however, may become feasible options in the next decade and can be considered for deployment in smaller configurations as supplementary options.

  3. Thermally induced phase changes, lateral heterogeneity of the mantle, continental roots, and deep slab anomalies

    NASA Technical Reports Server (NTRS)

    Anderson, Don L.

    1987-01-01

    Factors which influence the lateral heterogeneity in density and seismic velocity with depth in the upper earth mantle are discussed. It is emphasized that most of the increases in density and seismic velocity with depth are caused by pressure-induced solid-solid phase changes in the high-density high-velocity phases of mineral assemblage, due to variations in temperature. In particular, the ilmenite form of MgSiO3 and the gamma-spinel form of Mg2SiO4 have broad stability fields in cold mantle and are not stable in hotter mantle. It is emphasized that the density and velocity anomalies associated with temperature-induced phase changes in mineral assemblage must be taken into account in the thermal models of the slabs; when these effects are accounted for, the geoid and seismic anomalies associated with subducted slabs are consistent with slab confinement to the upper mantle and with layered models of mantle convection.

  4. Odor Profile of Different Varieties of Extra-Virgin Olive Oil During Deep Frying Using an Electronic Nose and SPME-GC-FID

    NASA Astrophysics Data System (ADS)

    Messina, Valeria; Biolatto, Andrea; Sancho, Ana; Descalzo, Adriana; Grigioni, Gabriela; de Reca, Noemí Walsöe

    2011-09-01

    The aim of the performed work was to evaluate with an electronic nose changes in odor profile of Arauco and Arbequina varieties of extra-virgin olive oil during deep-frying. Changes in odor were analyzed using an electronic nose composed of 16 sensors. Volatile compounds were analyzed by SPME-GC-FID. Principal Component Analysis was applied for electronic results. Arauco variety showed the highest response for sensors. Statistical analysis for volatile compounds indicated a significant (P<0.001) interaction between variety and time of frying processes. Arauco variety showed the highest production of volatile compounds at 60 min of deep frying. The two varieties presented distinct patterns of volatile products, being clearly identified with the electronic nose.

  5. 30 CFR 203.34 - To which production may an RSV earned by qualified phase 2 and phase 3 ultra-deep wells on my...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... perforated deeper than 15,000 feet TVD SS; (b) To production from a deep well or ultra-deep well on any other... condensate) volumes; or (d) To production from a deep well or ultra-deep well that commenced drilling before... 30 Mineral Resources 2 2012-07-01 2012-07-01 false To which production may an RSV earned...

  6. 30 CFR 203.34 - To which production may an RSV earned by qualified phase 2 and phase 3 ultra-deep wells on my...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... perforated deeper than 15,000 feet TVD SS; (b) To production from a deep well or ultra-deep well on any other... condensate) volumes; or (d) To production from a deep well or ultra-deep well that commenced drilling before... 30 Mineral Resources 2 2014-07-01 2014-07-01 false To which production may an RSV earned...

  7. 30 CFR 203.34 - To which production may an RSV earned by qualified phase 2 and phase 3 ultra-deep wells on my...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... feet TVD SS; (b) To production from a deep well or ultra-deep well on any other lease, except as...) To production from a deep well or ultra-deep well that commenced drilling before: (1) March 26, 2003... 30 Mineral Resources 2 2010-07-01 2010-07-01 false To which production may an RSV earned...

  8. 30 CFR 203.34 - To which production may an RSV earned by qualified phase 2 and phase 3 ultra-deep wells on my...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... perforated deeper than 15,000 feet TVD SS; (b) To production from a deep well or ultra-deep well on any other... condensate) volumes; or (d) To production from a deep well or ultra-deep well that commenced drilling before... 30 Mineral Resources 2 2011-07-01 2011-07-01 false To which production may an RSV earned...

  9. Variation of Clonal, Mesquite-Associated Rhizobial and Bradyrhizobial Populations from Surface and Deep Soils by Symbiotic Gene Region Restriction Fragment Length Polymorphism and Plasmid Profile Analysis

    PubMed Central

    Thomas, P. M.; Golly, K. F.; Zyskind, J. W.; Virginia, R. A.

    1994-01-01

    Genetic characteristics of 14 Rhizobium and 9 Bradyrhizobium mesquite (Prosopis glandulosa)-nodulating strains isolated from surface (0- to 0.5-m) and deep (4- to 6-m) rooting zones were determined in order to examine the hypothesis that surface- and deep-soil symbiont populations were related but had become genetically distinct during adaptation to contrasting soil conditions. To examine genetic diversity, Southern blots of PstI-digested genomic DNA were sequentially hybridized with the nodDABC region of Rhizobium meliloti, the Klebsiella pneumoniae nifHDK region encoding nitrogenase structural genes, and the chromosome-localized ndvB region of R. meliloti. Plasmid profile and host plant nodulation assays were also made. Isolates from mesquite nodulated beans and cowpeas but not alfalfa, clover, or soybeans. Mesquite was nodulated by diverse species of symbionts (R. meliloti, Rhizobium leguminosarum bv. phaseoli, and Parasponia bradyrhizobia). There were no differences within the groups of mesquite-associated rhizobia or bradyrhizobia in cross-inoculation response. The ndvB hybridization results showed the greatest genetic diversity among rhizobial strains. The pattern of ndvB-hybridizing fragments suggested that surface and deep strains were clonally related, but groups of related strains from each soil depth could be distinguished. Less variation was found with nifHDK and nodDABC probes. Large plasmids (>1,500 kb) were observed in all rhizobia and some bradyrhizobia. Profiles of plasmids of less than 1,000 kb were related to the soil depth and the genus of the symbiont. We suggest that interacting selection pressures for symbiotic competence and free-living survival, coupled with soil conditions that restrict genetic exchange between surface and deep-soil populations, led to the observed patterns of genetic diversity. Images PMID:16349226

  10. Variation of clonal, mesquite-associated rhizobial and bradyrhizobial populations from surface and deep soils by symbiotic gene region restriction fragment length polymorphism and plasmid profile analysis.

    PubMed

    Thomas, P M; Golly, K F; Zyskind, J W; Virginia, R A

    1994-04-01

    Genetic characteristics of 14 Rhizobium and 9 Bradyrhizobium mesquite (Prosopis glandulosa)-nodulating strains isolated from surface (0- to 0.5-m) and deep (4- to 6-m) rooting zones were determined in order to examine the hypothesis that surface- and deep-soil symbiont populations were related but had become genetically distinct during adaptation to contrasting soil conditions. To examine genetic diversity, Southern blots of PstI-digested genomic DNA were sequentially hybridized with the nodDABC region of Rhizobium meliloti, the Klebsiella pneumoniae nifHDK region encoding nitrogenase structural genes, and the chromosome-localized ndvB region of R. meliloti. Plasmid profile and host plant nodulation assays were also made. Isolates from mesquite nodulated beans and cowpeas but not alfalfa, clover, or soybeans. Mesquite was nodulated by diverse species of symbionts (R. meliloti, Rhizobium leguminosarum bv. phaseoli, and Parasponia bradyrhizobia). There were no differences within the groups of mesquite-associated rhizobia or bradyrhizobia in cross-inoculation response. The ndvB hybridization results showed the greatest genetic diversity among rhizobial strains. The pattern of ndvB-hybridizing fragments suggested that surface and deep strains were clonally related, but groups of related strains from each soil depth could be distinguished. Less variation was found with nifHDK and nodDABC probes. Large plasmids (>1,500 kb) were observed in all rhizobia and some bradyrhizobia. Profiles of plasmids of less than 1,000 kb were related to the soil depth and the genus of the symbiont. We suggest that interacting selection pressures for symbiotic competence and free-living survival, coupled with soil conditions that restrict genetic exchange between surface and deep-soil populations, led to the observed patterns of genetic diversity. PMID:16349226

  11. Serum Profiling of Rat Dermal Exposure to JP-8 Fuel Reveals an Acute-Phase Response.

    PubMed

    Larabee, Jason L; Hocker, James R; Cheung, John Y; Gallucci, Randle M; Hanas, Jay S

    2008-01-01

    ABSTRACT Dermal exposure to JP-8 petroleum jet fuel leads to toxicological responses in humans and rodents. Serum profiling is a molecular analysis of changes in the levels of serum proteins and other molecules in response to changes in physiology. This present study utilizes serum profiling approaches to examine biomolecular changes in the sera of rats exposed to dermal applications of JP-8 (jet propulsion fuel-8). Using gel electrophoresis and electrospray ionization (ESI) mass spectrometry (MS), levels of serum proteins as well as low-mass constituents were found to change after dermal exposures to JP-8. The serum protein levels altered included the acute-phase response proteins haptoglobin, ceruloplasmin, alpha(1)-inhibitor III, and apolipoprotein A-IV. Haptoglobin levels increased after a 1-day JP-8 dermal exposure and continued to increase through 7 days of exposure. Ceruloplasmin levels increased after 5 days of exposure. Serum alpha(1)-inhibitor III was reduced after a 1-day exposure and the depletion continued after 7 days of exposure. Apolipoprotein A-IV increased after a 1-day exposure and then returned to basal levels after 3- and 5-day exposures of JP-8. Levels of the acute-phase protein alpha(2)-macroglobulin were found to not vary over these time course studies. Using ESI-MS analysis directly on the sera from rats exposed to dermal JP-8, low-mass sera constituents were found to correlate with control (acetone) or JP-8 exposure. PMID:20020890

  12. Berry phases and profiles of line wings and rainbow satellites induced by optical collisions

    NASA Astrophysics Data System (ADS)

    Ciuryło, R.; Szudy, J.; Baylis, W. E.

    2015-09-01

    The concept of Berry phase is included in an analysis of the intensity distribution in far wings of pressure-broadened spectral lines emitted or absorbed by atoms placed in an external cone-rotating electric field. Particular attention is focused on frequency regions where rainbow satellite bands appear. A classical-path treatment that employs the time-dependent Schrödinger equation is used to derive an expression for the line shape, and it uses a dipole transition moment calculated with quasimolecular wave functions given by the Berry version of the adiabatic approximation. It is found that in the presence of an external rotating electric field, the intensity distribution in far wings can be expressed in terms of the universal line shape function of the unified Franck-Condon theory once energy shifts due to Stark and Berry effects are taken into account. We show that the influence of Berry phase in the profiles of the far wings can be manifested either in the form of deviations of observed profiles from the quasistatic distribution or the appearance of additional features in the vicinity of the maximum of the rainbow satellite band. As an example, the modification of the rainbow satellite at 162.3 nm in the red wing of the self-broadened Lyman-α line of hydrogen, caused by an external rotating electric field, is considered.

  13. Development of green betaine-based deep eutectic solvent aqueous two-phase system for the extraction of protein.

    PubMed

    Li, Na; Wang, Yuzhi; Xu, Kaijia; Huang, Yanhua; Wen, Qian; Ding, Xueqin

    2016-05-15

    Six kinds of new type of green betaine-based deep eutectic solvents (DESs) have been synthesized. Deep eutectic solvent aqueous two-phase systems (DES-ATPS) were established and successfully applied in the extraction of protein. Betaine-urea (Be-U) was selected as the suitable extractant. Single factor experiments were carried out to determine the optimum conditions of the extraction process, such as the salt concentration, the mass of DES, the separation time, the amount of protein, the temperature and the pH value. The extraction efficiency could achieve to 99.82% under the optimum conditions. Mixed sample and practical sample analysis were discussed. The back extraction experiment was implemented and the back extraction efficiency could reach to 32.66%. The precision experiment, repeatability experiment and stability experiment were investigated. UV-vis, FT-IR and circular dichroism (CD) spectra confirmed that the conformation of protein was not changed during the process of extraction. The mechanisms of extraction were researched by dynamic light scattering (DLS), the measurement of the conductivity and transmission electron microscopy (TEM). DES-protein aggregates and embraces phenomenon play considerable roles in the separation process. All of these results indicated that betaine-based DES-ATPS may provide a potential substitute new method for the separation of proteins. PMID:26992491

  14. GANSEKI: JAMSTEC Deep Seafloor Rock Sample Database Emerging to the New Phase

    NASA Astrophysics Data System (ADS)

    Tomiyama, T.; Ichiyama, Y.; Horikawa, H.; Sato, Y.; Soma, S.; Hanafusa, Y.

    2013-12-01

    Japan Agency for Marine-Earth Science and Technology (JAMSTEC) collects a lot of substantial samples as well as various geophysical data using its research vessels and submersibles. These samples and data, which are obtained by spending large amounts of human and physical resources, are precious wealth of the world scientific community. For the better use of these samples and data, it is important that they are utilized not only for initial purpose of each cruse but also for other general scientific and educational purposes of second-hand users. Based on the JAMSTEC data and sample handling policies [1], JAMSTEC has systematically stored samples and data obtained during research cruises, and provided them to domestic/foreign activities on research, education, and public relation. Being highly valued for second-hand usability, deep seafloor rock samples are one of the most important types of samples obtained by JAMSTEC, as oceanic biological samples and sediment core samples are. Rock samples can be utilized for natural history sciences and other various purposes; some of these purposes are connected to socially important issues such as earthquake mechanisms and mineral resource developments. Researchers and educators can access to JAMSTEC rock samples and associated data through 'GANSEKI [2]', the JAMSTEC Deep Seafloor Rock Sample Database. GANSEKI was established on the Internet in 2006 and its contents and functions have been continuously enriched and upgraded since then. GANSEKI currently provides 19 thousands of sample metadata, 9 thousands of collection inventory data and 18 thousands of geochemical data. Most of these samples are recovered from the North-western Pacific Ocean, although samples from other area are also included. The major update of GANSEKI held in May 2013 involved a replacement of database core system and a redesign of user interface. In the new GANSEKI, users can select samples easily and precisely using multi-index search, numerical

  15. Crustal structure and geodynamic of the Middle and Lower reaches of Yangtze metallogenic belt and neighboring areas: insights from deep seismic reflection profiling

    NASA Astrophysics Data System (ADS)

    Lu, Q.; Shi, D.; Liu, Z.; Zhang, Y.; Zhao, J.

    2014-12-01

    A 300 km deep seismic reflection profile across the middle and lower Yangtze River metallogenic belt (YRMB) and its adjacent areas established the architecture and geodynamic framework of the region. Results based on the interpretation of the deep seismic data include the deep complicated geometry of the Tan-Lu fault and Zhangbaling uplift, appears as a subvertical thrust fault with its deep portion dip toward the southeast, and along which the Zhangbaling uplift is squeezed out; complex upper crust deformation structure beneath Chuquan depression, within which there are both kink bands, thrusts, imbrication and fold structures reflecting contraction deformation, and detachment fault and normal-fault structures reflecting extensional deformation; the "crocodile" reflection structure emerging beneath the Tan-Lu fault and Ningwu-Lishui volcanic basin, i.e., the upper crust reflection thrust upward, and the lower crust reflection thrust downward and offsetting the Moho discontinuity, which reflects the decoupled deformation process of the upper and lower crust, and is interpreted as an intracontinental subduction. Further to the southeast, the upper crust deformation shows a large-scale "wave-form" pattern, making crustal scale syncline and anticline. The entire section of the reflection Moho is clearly discernible at depth of 30.0-34.5 km, and the Moho beneath the YRMB is shallowest, while the Moho beneath the North China block is deeper than that beneath the Yangtze block. Moho offsets could be seen beneath the Ningwu volcanic basin. Overall, the seismic data show evidence for an intracontinental orogeny and imposes constraints on the deep geodynamic model applied to study region. Our interpretation of seismic profile supports the view that the Yanshanian orogeny, due to the northwest subduction of the paleo-Pacific plate during the Middle-Late Jurassic, is the major event that shaped the tectonic framework of the region. A geodynamic model is proposed for the

  16. A two-moment cloud microphysics parameterization for mixed-phase clouds. Part 2: Maritime vs. continental deep convective storms

    NASA Astrophysics Data System (ADS)

    Seifert, A.; Beheng, K. D.

    2006-02-01

    A systematic modeling study investigates the effects of cloud condensation nuclei (CCNs) on the evolution of mixed-phase deep convective storms. Following previous studies the environmental conditions like buoyancy and vertical wind shear are varied to simulate different storm types like ordinary single cells, multicells and supercells. In addition, the CCN characteristics are changed from maritime to continental conditions. The results reveal very different effects of continentality on the cloud microphysics and dynamics of the different storms. While a negative feedback on total precipitation and maximum updraft velocity is found for ordinary single cells and supercell storms, a positive feedback exists for multicell cloud systems. The most important link between CCN properties, microphysics and dynamics is the release of latent heat of freezing.

  17. Periparturient cortisol, acute phase cytokine, and acute phase protein profiles of gilts housed in groups or stalls during gestation.

    PubMed

    Sorrells, A D; Eicher, S D; Harris, M J; Pajor, E A; Richert, B T

    2007-07-01

    Use of gestation stalls in pork production remains a controversial topic in animal welfare. Immune status and measures are frequently used to assess stress levels and thus well-being of confined animals. The important welfare issue of close confinement among gestating gilts was tested by quantifying cortisol, acute phase cytokine, and acute phase protein pro-files before and after farrowing of gilts housed in 2 systems. Landrace x Yorkshire crossbred gilts housed in groups of 4 (group, n = 8) in pens (3.9 x 2.4 m with 4 individual feeding spaces, 9.36 m(2) total or 2.34 m(2)/gilt) were compared with gilts housed in standard industry stalls (stall, n = 16; 2.2 x 0.6 m, 1.32 m(2)/gilt). Floors were fully slatted, and a substrate was not provided for either system. Cortisol was determined from saliva on d 105 of gestation, 1 h after moving the gilts into farrowing stalls (d 111), and 24 h and 7 d after farrowing. Cortisol was greater (P = 0.04) for group gilts compared with stall gilts 1 h after moving them into farrowing stalls and 24 h after farrowing. Cortisol concentrations decreased (P = 0.001) over time. Leukocyte mRNA expression of IL-1, IL-1 receptor antagonist, and tumor necrosis factor-alpha was determined by quantitative, reverse transcription PCR on d 35, 63, and 91 of gestation and 72 h after farrowing. Cytokine mRNA expression of peripheral blood mononuclear cells did not differ between housing systems for IL-1, its receptor antagonist, or for tumor necrosis factor-alpha. Acute phase proteins, including fibrinogen, haptoglobin, and alpha(1)-acid glycoprotein were determined for plasma samples taken at d 35, 63, and 91 of gestation and 72 h and 14 d after farrowing. In contrast to cortisol, plasma fibrinogen concentrations increased (P < 0.005) over time. Haptoglobin did not differ between treatments (P > 0.10). Stall gilts tended to have greater (P = 0.07) plasma alpha(1)-acid glycoprotein concentrations than group animals at d 35 of gestation and d 14

  18. Comparison of Vibroseis and explosive source methods for deep crustal seismic reflection profiling in the Basin and Range province

    USGS Publications Warehouse

    Brocher, T.M.; Hart, P.E.

    1991-01-01

    Direct comparison of low-fold, high-energy explosive and high-fold, lower-energy Vibroseis methods for acquiring deep crustal seismic reflection data in the Basin and Range Province suggests that the high-fold common midpoint (CMP) method there does not provide the best possible image of lower crustal structure. -from Authors

  19. SinoProbe-02:Deep Seismic Reflection Profile (480km) experiment in North China:Acquisition and the Preliminary Processing result

    NASA Astrophysics Data System (ADS)

    Hou, H.; Gao, R.; Li, Q.; Li, W.; Kuang, Z.; Liu, J.; Zhang, J.; Guan, Y.; Keller, G.; Liu, M.; Cox, C. M.; Holloway, S.; Chang, J. C.; Kaip, G. M.; Zhang, S.

    2010-12-01

    An active-source deep seismic reflection experiment was recorded across the Central Asian and Trans-North China orogens (see Figure) last winter as part of the SinoProbe-02 program. This profile extended southeastward from Erenhot and ended in the Huailai basin near Beijing. The shot depth was 25m; the shot size was 30-50kg; and the nominal shot interval was 250 km. Additional 200-kg charges were set off every 1 km and 1 ton shots were placed at intervals of 50 km. A Sercel 408 XL recording system and 2000 strings of SM-24 geophones were deployed at a spacing of 50m. Recording was at a 2ms sample interval for a total of 30s.The typical processing stream included crooked-line binning, refraction statics, true amplitude recovery, deconvolution, detailed velocity analyses, residual statics corrections and NMO stack (see Table). To the south, a transparent granite body is indicated in the middle crust with a series of north dipping reflections structures in the lower crust. The complex structures near Sonid Youqi suggest a transparent crust due to the Solonker suture zone. Map showing deep seismic reflection profile (solid black line) across North China. Processing Parameters

  20. Phase and Size Control of Core-Shell Upconversion Nanocrystals Light up Deep Dual Luminescence Imaging and CT In Vivo.

    PubMed

    Kang, Ning; Liu, Yu; Zhou, Yaming; Wang, Dong; Chen, Chuan; Ye, Shefang; Nie, Liming; Ren, Lei

    2016-06-01

    Upconversion nanocrystals (UCNCs) have recently been explored as optical imaging nanoprobes. However, conventional β-NaLuF4 (-) based UCNCs often suffer from large particle size and weak upconversion luminescence (UCL) intensity, leading to poor biocompatibility and low detection sensitivity. Here, a novel strategy for controlling the crystalline phase and size of UCNCs has been developed by doping of yttrium ions, resulting in particle size reduction and phase transition. The total UCL intensity of prepared core-shell UCNCs is significantly enhanced up to ≈4.9 and ≈17.4 times after Tm(3+) and Er(3+) doping than that of core UCNCs, offering deeper tissue UCL imaging with a depth of 8 mm in vivo. Moreover, the CT signal of core-shell UCNCs is ≈1.5 and ≈3.5 times brighter than that of core UCNCs and commercial ioversol agent because of increasing contents of Lu(3+) doped in UCNCs. The synthesized core-shell UCNCs hold a great promise in deep UCL and CT dual-modality imaging in vitro and in vivo. PMID:26990395

  1. An ultrasound cylindrical phased array for deep heating in the breast: theoretical design using heterogeneous models

    NASA Astrophysics Data System (ADS)

    Bakker, J. F.; Paulides, M. M.; Obdeijn, I. M.; van Rhoon, G. C.; van Dongen, K. W. A.

    2009-05-01

    The objective of this theoretical study is to design an ultrasound (US) cylindrical phased array that can be used for hyperthermia (40-44 °C) treatment of tumours in the intact breast. Simultaneously, we characterize the influence of acoustic and thermal heterogeneities on the specific absorption rate (SAR) and temperature patterns to determine the necessity of using heterogeneous models for a US applicator design and treatment planning. Cylindrical configurations of monopole transducers are studied on their ability to generate interference patterns that can be steered electronically to the location of the target region. Hereto, design parameters such as frequency, number of transducers per ring, ring distance and number of rings are optimized to obtain a small primary focus, while suppressing secondary foci. The models account for local heterogeneities in both acoustic (wave velocity and absorption) and thermal (blood perfusion rate, heat capacity and conductivity) tissue properties. We used breast models with a central tumour (30 × 20 × 38 mm3) and an artificial thorax tumour (sphere with a radius of 25 mm) to test the design. Simulations predict that a US cylindrical phased array, consisting of six rings with 32 transducers per ring, a radius of 75 mm and 66 mm distance between the first and sixth transducer ring, operating at a frequency of 100 kHz, can be used to obtain 44 °C in the centre of tumours located anywhere in the intact breast. The dimensions of the volumes enclosed by the 41 °C iso-temperature are 19 × 19 × 21 mm3 and 21 × 21 × 32 mm3 for the central and the thorax tumours, respectively. It is demonstrated that acoustic and thermal heterogeneities do not disturb the SAR and temperature patterns.

  2. Improvements in the vapor-time profile analysis of explosive odorants using solid-phase microextraction.

    PubMed

    Young, Mimy; Schantz, Michele; MacCrehan, William

    2016-07-15

    A modified approach for characterization of the vapor-time profile of the headspace odors of explosives was developed using solid-phase microextraction (SPME) incorporating introduction of an externally-sampled internal standard (ESIS) followed by gas chromatography/mass spectrometry (GC/MS) analysis. With this new method, reproducibility of the measurements of 2-ethyl-1-hexanol and cyclohexanone were improved compared to previous work (Hoffman et al., 2009; Arthur and Pawliszyn, 1990) through the use of stable-isotope-labeled internal standards. Exposing the SPME fiber to the ESIS after sampling the target analyte proved to be advantageous, while still correcting for fiber variability and detector drift. For the analysis of high volatility compounds, incorporation of the ESIS using the SPME fiber in the retracted position minimized the subsequent competitive loss of the target analyte, allowing for much longer sampling times. PMID:27286650

  3. Nocturnal Melatonin Profiles in Patients with Delayed Sleep-Wake Phase Disorder and Control Sleepers.

    PubMed

    Micic, Gorica; Lovato, Nicole; Gradisar, Michael; Burgess, Helen J; Ferguson, Sally A; Kennaway, David J; Lack, Leon

    2015-10-01

    A significant delay in the timing of endogenous circadian rhythms has been associated with delayed sleep phase disorder (DSPD). More recently, other mechanisms have also been proposed to account for this disorder. To further explore the etiology of DSPD, the present study compared nocturnal melatonin profiles of 26 DSPD patients (18 males, 8 females; age, 21.73 ± 4.98 years) and 17 normally timed good sleepers (10 males, 7 females; age, 23.82 ± 5.23 years) in a time-free, dim-light (<10 lux) laboratory environment. A 30-h modified constant routine with alternating 20-min sleep opportunities and 40 min of enforced wakefulness was used to measure the endogenous melatonin circadian rhythm. Salivary melatonin was sampled half-hourly from 1820 h to 0020 h and then hourly from 0120 h to 1620 h. DSPD patients had significantly later timed melatonin profiles that were delayed by approximately 3 h compared to normal sleepers, and there were no notable differences in the relative duration of secretion between groups. However, melatonin secretion between dim-light melatonin onset (DLMO) and acrophase was less prominent in DSPD patients compared to good sleepers, who showed a more acute initial surge of melatonin following the DLMO. Although the regulatory role of melatonin is unknown, abnormal melatonin profiles have been linked to psychiatric and neurological disorders (e.g., major depression, obsessive compulsive disorder, Parkinson disease). These results therefore suggest that in addition to a delayed endogenous circadian rhythm, a diminished initial surge of melatonin secretion following DLMO may contribute to the etiology of DSPD. PMID:26149168

  4. Measurement of a fiber-end surface profile by use of phase-shifting laser interferometry

    NASA Astrophysics Data System (ADS)

    Wang, Shihua; Quan, Chenggen; Tay, Cho Jui; Reading, Ivan; Fang, Zhongping

    2004-01-01

    We describe a laser interferometric system in which two objectives are used to measure surface profile on a connectorized fiber-end surface. By the use of the proposed illumination design a He-Ne laser as a point light source is transformed to an extended light source, which is beneficial to localize interference fringe pattern near the test surface. To obtain an optimal contrast of the interference fringe pattern, the flat mirror with an adjustable reflection ratio is used to suit different test surfaces. A piezoelectric transducer attached on the reference mirror can move precisely along the optical axis of the objective and permits implementation of four-step phase-shifting interferometry without changing the relative position between the CCD sensor and the test surface. Therefore, an absolutely constant optical magnification can be accurately kept to capture the interference fringe patterns resulting from a combination of light reflected from both the reference flat mirror and the test surface. The experimental result shows that surface profile on a fiber-end with surface features such as a small fiber diameter of 125 μm and a low reflection ratio of less than 4% are measurable. Measurements on a standard calibration ball show that the accuracy of the proposed setup is comparable with that of existing white-light interferometers and stylus profilometers.

  5. Phase retrieval from a single interferometric pattern to determine the profile caused by laser ablation on spherical surfaces

    NASA Astrophysics Data System (ADS)

    Rodríguez-Rodríguez, M. I.; López-Olazagasti, E.; Rosales, M. A.; Ibarra, Jorge; Tepichín, E.

    2009-08-01

    We have been working in the interferometric analysis of the ablation profile obtained with different techniques of refractive surgery, applied directly on hard contact lenses. We have demonstrated qualitatively that different ablations produce different fringe patterns; implying different focal shifts1. These results were obtained by means of a Mach- Zehnder type interferometer, where we used a similar unablated contact lens as a reference. Due to the size of each sample, it is difficult to get different fringe patterns with different phase factors. Therefore, the typical phase shifting methods are not suitable in our case. To determine the corresponding profile caused by the different ablation techniques we applied in this work the interpolation method that provide an analysis of static fringe patterns. This method of phase retrieval allows us to obtain the PSF and MTF related to each profile. The advantage of this procedure is that we can obtain a time invariant performance of the resulting ablated surface.

  6. High-accuracy inspection of defects and profile of wafers by phase measuring deflectometry

    NASA Astrophysics Data System (ADS)

    Yue, Huimin; Wu, Yuxiang; Zhao, Biyu; Ou, Zhonghua; Liu, Yong

    2014-09-01

    The demands of the less-defective and high-flatness wafers are urgent in many wafer based technologies ranging from micro-electronics to the current photovoltaic industry. As the wafer becomes thinner and larger to cope with the advances in those industries, there is an increasing possibility of the emerging of crack and warp on the wafer surface. High-accuracy inspection of defects and profile are thus necessary to ensure the reliability of device. Phase measuring deflectometry(PMD) is a fast, cost-effective and high accuracy measurement technology which has been developed in recent years. As a slope measurement technology, PMD possesses a high sensitivity. Very small slope variation will lead to a large variation of the phase. PMD is very possible to have a good performance in the wafer inspection. In this paper, the requirements of the wafer inspection in the industries are discussed, and compatibility of PMD and those requirements is analyzed. In the experimental work, PMD gets the slope information of the wafer surface directly. The curvature or height information can be acquired simply by the derivation or integral of the slope. PMD is proved to make a superior result in high-precision defect detecting and shape measurement of wafer by the analysis of experiment results.

  7. Solid phase extraction and metabolic profiling of exudates from living copepods

    PubMed Central

    Heuschele, Jan; Nylund, Göran M.; Pohnert, Georg; Pavia, Henrik; Bjærke, Oda; Pender-Healy, Larisa A.; Tiselius, Peter; Kiørboe, Thomas

    2016-01-01

    Copepods are ubiquitous in aquatic habitats. They exude bioactive compounds that mediate mate finding or induce defensive traits in prey organisms. However, little is known about the chemical nature of the copepod exometabolome that contributes to the chemical landscape in pelagic habitats. Here we describe the development of a closed loop solid phase extraction setup that allows for extraction of exuded metabolites from live copepods. We captured exudates from male and female Temora longicornis and analyzed the content with high resolution LC-MS. Chemometric methods revealed 87 compounds that constitute a specific chemical pattern either qualitatively or quantitatively indicating copepod presence. The majority of the compounds were present in both female and male exudates, but nine compounds were mainly or exclusively present in female exudates and hence potential pheromone candidates. Copepodamide G, known to induce defensive responses in phytoplankton, was among the ten compounds of highest relative abundance in both male and female extracts. The presence of copepodamide G shows that the method can be used to capture and analyze chemical signals from living source organisms. We conclude that solid phase extraction in combination with metabolic profiling of exudates is a useful tool to develop our understanding of the chemical interplay between pelagic organisms. PMID:26788422

  8. New long trace profiler based on phase plate diffraction for optical metrology of SSRF

    SciTech Connect

    Zeng Danhua; Xiao Tiqiao; Du Guohao; Wen Li; Luo Hongxin; Xia Shaojian; Xu Hongjie

    2006-09-15

    A long trace profiler LTP-1200, with a novel f-{theta} system based on phase plate diffraction and a scanning range up to 1200 mm, has been developed at Shanghai Synchrotron Radiation Facility. The central dark line in the diffraction pattern generated by a {pi} phase plate is taken as the positioning benchmark. A magnet levitated linear rail with very high accuracy is used. A granite bench is employed to reduce deformation due to self-gravity of the rail. The focused diffraction pattern is recorded with an area charge-coupled device. The generalized regression neural network algorithm is adopted to improve the beam positioning precision. The static stability of LTP-1200 in 5 h is 0.14 {mu}rad, and the repeatability reaches 0.05 {mu}rad in a common laboratory without any special control of temperature, air turbulence, etc. Calibration tests were carried out with a high precision autocollimator and a standard spherical mirror, respectively. Results show the high performance and reliability of the LTP-1200.

  9. The role of TiO 2 phases during melting of subduction-modified crust: Implications for deep mantle melting

    NASA Astrophysics Data System (ADS)

    Bromiley, Geoffrey D.; Redfern, Simon A. T.

    2008-03-01

    Partitioning of Nb, Ta, Hf and Zr between rutile, its high-pressure polymorph TiO2(II) and silicate melt has been experimentally determined at 2 GPa/1200 °C, 6 GPa/1600 °C, 8 GPa/1800 °C and 10 GPa/1900 °C. Results show that characteristic depletion of Nb and Ta in partial melts due to the presence of rutile in solid residues (for example during melting of subducting oceanic crust) is strongly dependent on depth of partial melting. With increasing pressure, changes in melt structure result in marked reduction in Dmin/melt for Nb (14.8, 5.4, 2.5, and 2.4 with increasing P/T) and Ta (28.0, 17.0, 6.9, and 5.5). A strong pressure effect is also noted in Dmin/melt for Zr (2.1, 0.6, 0.9, and 1.2) and Hf (4.1, 0.9, 1.3, and 1.3), although for these elements the rutile to TiO2(II) transition also influences partitioning behaviour. Results have important implications for melting of oceanic crust in Earth's deep mantle. Ancient subduction-modified crust cannot be a direct source for ocean-island basalts (OIB) unless depth of melting is greater than 300 km, or degree of partial melting is much higher than suggested on the basis of previous trace element modeling work (and sufficient to remove TiO2 phases from solid residues). Likewise, the absence of strong depletion of Nb and Ta in OIB also provides constraints on degree of partial melting vs depth of partial melting for models where melting of ancient crust acts as an indirect source for OIB by metasomatic interaction with the mantle. The controlling influence of melt structure on partitioning behaviour of high-field strength elements (HFSE) implies that relative enrichment of Nb and Ta and reduction in Zr/Nb in high-pressure partial melts should occur even when TiO2 phases are not present in solid residues. As such, depth of partial melting may be as important a factor as mineral and melt chemistry and degree of partial melting in constraining the composition of partial melts from Earth's deep interior.

  10. Evaluation of an Extended Autocorrelation Phase Estimator for Ultrasonic Velocity Profiles Using Nondestructive Testing Systems.

    PubMed

    Ofuchi, César Yutaka; Coutinho, Fabio Rizental; Neves, Flávio; de Arruda, Lucia Valéria Ramos; Morales, Rigoberto Eleazar Melgarejo

    2016-01-01

    In this paper the extended autocorrelation velocity estimator is evaluated and compared using a nondestructive ultrasonic device. For this purpose, three velocity estimators are evaluated and compared. The autocorrelation method (ACM) is the most used and well established in current ultrasonic velocity profiler technology, however, the technique suffers with phase aliasing (also known as the Nyquist limit) at higher velocities. The cross-correlation method (CCM) is also well known and does not suffer with phase aliasing as it relies on time shift measurements between emissions. The problem of this method is the large computational burden due to several required mathematical operations. Recently, an extended autocorrelation method (EAM) which combines both ACM and CCM was developed. The technique is not well known within the fluid engineering community, but it can measure velocities beyond the Nyquist limit without the ACM phase aliasing issues and with a lower computational cost than CCM. In this work, all three velocity estimation methods are used to measure a uniform flow of the liquid inside a controlled rotating cylinder. The root-mean-square deviation variation coefficient (CVRMSD) of the velocity estimate and the reference cylinder velocity was used to evaluate the three different methods. Results show that EAM correctly measures velocities below the Nyquist limit with less than 2% CVRMSD. Velocities beyond the Nyquist limit are only measured well by EAM and CCM, with the advantage of the former of being computationally 15 times faster. Furthermore, the maximum value of measurable velocity is also investigated considering the number of times the velocity surpasses the Nyquist limit. The combination of number of pulses and number of samples, which highly affects the results, are also studied in this work. Velocities up to six times the Nyquist limit could be measurable with CCM and EAM using a set of parameters as suggested in this work. The results validate

  11. HPV Population Profiling in Healthy Men by Next-Generation Deep Sequencing Coupled with HPV-QUEST.

    PubMed

    Yin, Li; Yao, Jin; Chang, Kaifen; Gardner, Brent P; Yu, Fahong; Giuliano, Anna R; Goodenow, Maureen M

    2016-02-01

    Multiple-type human papillomaviruses (HPV) infection presents a greater risk for persistence in asymptomatic individuals and may accelerate cancer development. To extend the scope of HPV types defined by probe-based assays, multiplexing deep sequencing of HPV L1, coupled with an HPV-QUEST genotyping server and a bioinformatic pipeline, was established and applied to survey the diversity of HPV genotypes among a subset of healthy men from the HPV in Men (HIM) Multinational Study. Twenty-one HPV genotypes (12 high-risk and 9 low-risk) were detected in the genital area from 18 asymptomatic individuals. A single HPV type, either HPV16, HPV6b or HPV83, was detected in 7 individuals, while coinfection by 2 to 5 high-risk and/or low-risk genotypes was identified in the other 11 participants. In two individuals studied for over one year, HPV16 persisted, while fluctuations of coinfecting genotypes occurred. HPV L1 regions were generally identical between query and reference sequences, although nonsynonymous and synonymous nucleotide polymorphisms of HPV16, 18, 31, 35h, 59, 70, 73, cand85, 6b, 62, 81, 83, cand89 or JEB2 L1 genotypes, mostly unidentified by linear array, were evident. Deep sequencing coupled with HPV-QUEST provides efficient and unambiguous classification of HPV genotypes in multiple-type HPV infection in host ecosystems. PMID:26821041

  12. HPV Population Profiling in Healthy Men by Next-Generation Deep Sequencing Coupled with HPV-QUEST

    PubMed Central

    Yin, Li; Yao, Jin; Chang, Kaifen; Gardner, Brent P.; Yu, Fahong; Giuliano, Anna R.; Goodenow, Maureen M.

    2016-01-01

    Multiple-type human papillomaviruses (HPV) infection presents a greater risk for persistence in asymptomatic individuals and may accelerate cancer development. To extend the scope of HPV types defined by probe-based assays, multiplexing deep sequencing of HPV L1, coupled with an HPV-QUEST genotyping server and a bioinformatic pipeline, was established and applied to survey the diversity of HPV genotypes among a subset of healthy men from the HPV in Men (HIM) Multinational Study. Twenty-one HPV genotypes (12 high-risk and 9 low-risk) were detected in the genital area from 18 asymptomatic individuals. A single HPV type, either HPV16, HPV6b or HPV83, was detected in 7 individuals, while coinfection by 2 to 5 high-risk and/or low-risk genotypes was identified in the other 11 participants. In two individuals studied for over one year, HPV16 persisted, while fluctuations of coinfecting genotypes occurred. HPV L1 regions were generally identical between query and reference sequences, although nonsynonymous and synonymous nucleotide polymorphisms of HPV16, 18, 31, 35h, 59, 70, 73, cand85, 6b, 62, 81, 83, cand89 or JEB2 L1 genotypes, mostly unidentified by linear array, were evident. Deep sequencing coupled with HPV-QUEST provides efficient and unambiguous classification of HPV genotypes in multiple-type HPV infection in host ecosystems. PMID:26821041

  13. Molecularly imprinted polymers combination with deep eutectic solvents for solid-phase extraction of caffeic acid from hawthorn.

    PubMed

    Li, Guizhen; Tang, Weiyang; Cao, Weimin; Wang, Qian; Zhu, Tao

    2015-08-01

    Molecularly imprinted polymers (MIPs) with caffeic acid as template and non-imprinted polymers (NIPs) materials were prepared in the same procedure. Field emission scanning electron microscopy (FE-SEM) and adsorption capacity test were used to evaluate characteristic of the new materials. MIPs, NIPs and C18 were used for rapid purification of caffeic acid from hawthorn with solid-phase extraction ( SPE) , and extract yields of caffeic acid with the proposed materials were 3.46 µg/g, 1.01 µg/g and 1.17 µg/g, respectively. To optimize the MIPs-SPE procedures, different kinds of elution solutions were studied. Deep eutectic solvents (DESs) were prepared by choline chloride (ChCl)-glycerol (1/2, n/n) and choline chloride-urea (1/ 2, n/n). Methanol was mixed with the two kinds of DESs (glycerol-based DESs, urea-based DESs) in different ratios (0.5:1, 1:1, 2:1, 3:1, 4:1, 5:1, v/v), and they were used to investigated as elution solutions in the above MIPs-SPE procedures. The results showed that MIPs were potential SPE materials, and methanol/ glycerol-based DESs (3 :1, v/v) had the best elution capability with the recovery of 82.32%. PMID:26749853

  14. Study of cosmic-ray modulation during the recent deep solar minimum, mini maximum and intervening ascending phase of solar cycle 24

    NASA Astrophysics Data System (ADS)

    Badruddin, B.; Aslam, O. P. M.

    After a prolonged and deep solar minimum at the end of cycle 23, current solar cycle 24 is one of the very low active cycles, weakest cycle in more than 50 years. These two periods of deep minima and mini maxima are separated by a period of increasing solar activity as measured by sunspot numbers. We study the cosmic ray relationship with the solar activity, heliospheric plasma and field parameters including the heliospheric current sheet (HCS), during these three periods (phases) of different level and nature of solar activity; (a) a deep minimum, (b) an increasing period and (c) a ‘mini’ maximum. We utilize the neutron monitor data from stations located around the globe to study the rigidity dependence of modulation during the two extremes, i.e., minima and maxima. We also study the time lag between the GCR intensity and various solar/interplanetary parameters separately during the three activity phases. Using the cosmic ray data of neutron monitors with different cutoff rigidities, we study the rigidity dependence of time lag during individual phases. The role/effectiveness of various parameters, including the HCS tilt, in modulating the GCR intensity during the three different phases has also been studied by correlation analysis. The relative importance of various physical processes during different phases and the implication of these results for modulation models are also discussed.

  15. Into the Deep: Variability in Soil Microbial Communities and Carbon Turnover Along a Tropical Forest Soil Depth Profile

    NASA Astrophysics Data System (ADS)

    Pett-Ridge, J.; McFarlane, K. J.; Heckman, K. A.; Reed, S.; Wood, T. E.

    2015-12-01

    Tropical forest soils store more carbon (C) than any other terrestrial ecosystem and exchange vast amounts of CO2, water, and energy with the atmosphere. Much of this C is leached and stored within deeper soil layers, but we know exceedingly little about the fate of this C or the microbial communities that drive deep soil biogeochemistry. From the data that do exist, most organic matter (OM) in tropical soils appears associated with mineral particles, suggesting deep soils may provide greater C stabilization due to organo-metal co-precipitation and mineral-surface interactions. However, few studies have evaluated sub-surface soils in tropical ecosystems, the turnover times of deep soil C, and sensitivity of this C to global environmental change. To address this critical research need, we quantified C pools, microbial communities and soil radiocarbon turnover times in bulk soils and soil fractions [free light (unprotected), dense (mineral-associated)] from 0-140 cm in replicate soil pits in the Luquillo Experimental Forest, Puerto Rico. Unsurprisingly, we found soil C, nitrogen, and root and microbial biomass all declined exponentially with depth; total C stocks dropped from 5.5 % at the surface to <0.5% at 140cm depth. Soil OM 14C and mean turnover times were variable across replicate horizons, ranging from 3-1500 years at the surface (0-20 cm), to 5000-40,000 years at 140 cm depth. Soil C in the mineral associated fraction was much older than the free light fraction C, which reflected modern 14C at all depths. In comparison to temperate deciduous forests, these 14C values reflect far older soil C, and OM decomposition that highly favors free light C pools, even at depth. While previous work suggests these low C tropical subsoils contain small but metabolically active microbial communities at depths of ~100cm, these organisms appear highly OM limited, and preferentially degrade recent inputs. In the coming half century, tropical forests are predicted to see a 2 - 5

  16. Design and analysis of low-loss linear analog phase modulator for deep space spacecraft X-band transponder (DST) application

    NASA Technical Reports Server (NTRS)

    Mysoor, Narayan R.; Mueller, Robert O.

    1991-01-01

    This paper summarizes the design concepts, analyses, and the development of an X-band transponder low-loss linear phase modulator for deep space spacecraft applications. A single section breadboard circulator-coupled reflection phase modulator has been analyzed, fabricated, and evaluated. Two- and three-cascaded sections have been modeled and simulations performed to provide an X-band DST phase modulator with +/- 2.5 radians of peak phase deviation to accommodate down-link signal modulation with composite telemetry data and ranging with a deviation linearity tolerance +/- 8 percent and insertion loss of less than 10 +/- 0.5 dB. A two-section phase modulator using constant gamma hyperabrupt varactors and an efficient modulator driver circuit was breadboarded. The measured results satisfy the DST phase modulator requirements, and excellent agreement with the predicted results.

  17. Cytokine Profile of Children Hospitalized with Virologically-Confirmed Dengue during Two Phase III Vaccine Efficacy Trials

    PubMed Central

    Harenberg, Anke; de Montfort, Aymeric; Jantet-Blaudez, Frédérique; Bonaparte, Matthew; Boudet, Florence; Saville, Melanie; Jackson, Nicholas; Guy, Bruno

    2016-01-01

    Background Two large-scale efficacy studies with the recombinant yellow fever-17D–dengue virus, live-attenuated, tetravalent dengue vaccine (CYD-TDV) candidate undertaken in Asia (NCT01373281) and Latin America (NCT01374516) demonstrated significant protection against dengue disease during two years’ active surveillance (active phase). Long-term follow up of participants for breakthrough disease leading to hospitalization is currently ongoing (hospital phase). Methodology/Principal findings We assessed the cytokine profile in acute sera from selected participants hospitalized (including during the active phase) up to the beginning of the second year of long-term follow up for both studies. The serum concentrations of 38 cytokines were measured in duplicate using the Milliplex Human Cytokine MAGNETIC BEAD Premixed 38 Plex commercial kit (Millipore, Billerica, MA, USA). Partial least squares discriminant analyses did not reveal any difference in the overall cytokine profile of CYD-TDV and placebo recipients hospitalized for breakthrough dengue regardless of stratification used. In addition, there was no difference in the cytokine profile for breakthrough dengue among those aged <9 years versus those aged ≥ 9 years. Conclusions/Significance These exploratory findings show that CYD-TDV does not induce a particular immune profile versus placebo, corroborating the clinical profile observed. PMID:27459266

  18. Chemical profile of the secondary metabolites produced by a deep-sea sediment-derived fungus Penicillium commune SD-118

    NASA Astrophysics Data System (ADS)

    Shang, Zhuo; Li, Xiaoming; Meng, Li; Li, Chunshun; Gao, Shushan; Huang, Caiguo; Wang, Bingui

    2012-03-01

    Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X ( 1), and 14 other known compounds comprising three steroids ( 2-4), two ceramides ( 5 and 6), six aromatic compounds ( 7-12), and three alkaloids ( 13-15). Xanthocillin X ( 1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X ( 1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin ( 15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X ( 1).

  19. Crustal structure and geodynamics of the Middle and Lower reaches of Yangtze metallogenic belt and neighboring areas: Insights from deep seismic reflection profiling

    NASA Astrophysics Data System (ADS)

    Lü, Qingtian; Shi, Danian; Liu, Zhendong; Zhang, Yongqian; Dong, Shuwen; Zhao, Jinhua

    2015-12-01

    A 300 km long seismic reflection profile was acquired across the Middle and Lower Reaches of the Yangtze River (MLY) metallogenic belt and its adjacent areas. The objective of the survey was to establish the deep architecture and geodynamic framework of the region. Results based on the interpretation of the deep seismic data include (1) Tan-Lu fault appears as a subvertical thrust fault or transpression fault with its deep portion dipping toward the southeast; (2) the Zhangbaling uplift is squeezed out along this fault; (3) complex upper crustal deformation structures beneath the Chuquan depression include both kink bands, thrusts, imbrication and fold structures reflecting contraction deformation, and detachment fault and normal-fault structures reflecting extensional deformation; (4) the "crocodile" reflection structure emerging beneath the Tan-Lu fault and Ningwu-Lishui volcanic basin, which represents the decoupled deformation process of the upper and lower crust associated with intra-continental subduction; (5) further to the southeast, the upper crust deformation shows a large-scale "wave-form" pattern, making crustal scale syncline and anticline; (6) the entire section of the reflection Moho is clearly discernible at depth of 30.0-34.5 km, and the Moho beneath the Middle and Lower Reaches of Yangtze River metallogenic belt is shallowest, while the Moho beneath the North China block is deeper than that beneath the Yangtze block. The Moho offsets could be seen beneath the Ningwu volcanic basin. The seismic reflection data suggest that lithosphere delamination and asthenosphere upwelling that may result from the Mesozoic intra-continental orogenesis is responsible for the formation of large scale magmatism and mineralization in the MLY metallogenic belt.

  20. Theoretical Analysis of Effects of Deep Level, Back Contact, and Absorber Thickness on Capacitance-Voltage Profiling of CdTe Thin-Film Solar Cells

    SciTech Connect

    Li, J. V.; Halverson, A. F.; Sulima, O. V.; Bansal, S.; Burst, J. M.; Barnes, T. M.; Gessert, T. A.; Levi, D. H.

    2012-05-01

    The apparent carrier density profile measured by the capacitance-voltage technique in CdTe thin-film solar cells frequently displays a distinctive U-shape. We show that, even assuming a uniform carrier density, such a U-shape may arise from deep levels, a non-ohmic back-contact, and a thin absorber, which are commonly present in practical CdTe thin-film solar cells. A thin CdTe absorber contributes to the right branch of the U-shape due to a punch-through effect at reverse or zero biases, when the CdTe absorber is nearly fully depleted. A rectifying back-contact contributes to both branches of the U-shape due to voltage sharing with the front junction under a forward bias and early punch-through under a reverse bias. Deep levels contribute to the right branch, but also raise the bottom of the U-shape, leading to an overestimate of carrier density.

  1. MiRGator v3.0: a microRNA portal for deep sequencing, expression profiling and mRNA targeting.

    PubMed

    Cho, Sooyoung; Jang, Insu; Jun, Yukyung; Yoon, Suhyeon; Ko, Minjeong; Kwon, Yeajee; Choi, Ikjung; Chang, Hyeshik; Ryu, Daeun; Lee, Byungwook; Kim, V Narry; Kim, Wankyu; Lee, Sanghyuk

    2013-01-01

    Biogenesis and molecular function are two key subjects in the field of microRNA (miRNA) research. Deep sequencing has become the principal technique in cataloging of miRNA repertoire and generating expression profiles in an unbiased manner. Here, we describe the miRGator v3.0 update (http://mirgator.kobic.re.kr) that compiled the deep sequencing miRNA data available in public and implemented several novel tools to facilitate exploration of massive data. The miR-seq browser supports users to examine short read alignment with the secondary structure and read count information available in concurrent windows. Features such as sequence editing, sorting, ordering, import and export of user data would be of great utility for studying iso-miRs, miRNA editing and modifications. miRNA-target relation is essential for understanding miRNA function. Coexpression analysis of miRNA and target mRNAs, based on miRNA-seq and RNA-seq data from the same sample, is visualized in the heat-map and network views where users can investigate the inverse correlation of gene expression and target relations, compiled from various databases of predicted and validated targets. By keeping datasets and analytic tools up-to-date, miRGator should continue to serve as an integrated resource for biogenesis and functional investigation of miRNAs. PMID:23193297

  2. Remote Sensing the Vertical Profile of Cloud Droplet Effective Radius, Thermodynamic Phase, and Temperature

    NASA Technical Reports Server (NTRS)

    Martins, J. V.; Marshak, A.; Remer, L. A.; Rosenfeld, D.; Kaufman, Y. J.; Fernandez-Borda, R.; Koren, I.; Correia, A. L.; Zubko, V.; Artaxo, P.

    2011-01-01

    Cloud-aerosol interaction is a key issue in the climate system, affecting the water cycle, the weather, and the total energy balance including the spatial and temporal distribution of latent heat release. Information on the vertical distribution of cloud droplet microphysics and thermodynamic phase as a function of temperature or height, can be correlated with details of the aerosol field to provide insight on how these particles are affecting cloud properties and their consequences to cloud lifetime, precipitation, water cycle, and general energy balance. Unfortunately, today's experimental methods still lack the observational tools that can characterize the true evolution of the cloud microphysical, spatial and temporal structure in the cloud droplet scale, and then link these characteristics to environmental factors and properties of the cloud condensation nuclei. Here we propose and demonstrate a new experimental approach (the cloud scanner instrument) that provides the microphysical information missed in current experiments and remote sensing options. Cloud scanner measurements can be performed from aircraft, ground, or satellite by scanning the side of the clouds from the base to the top, providing us with the unique opportunity of obtaining snapshots of the cloud droplet microphysical and thermodynamic states as a function of height and brightness temperature in clouds at several development stages. The brightness temperature profile of the cloud side can be directly associated with the thermodynamic phase of the droplets to provide information on the glaciation temperature as a function of different ambient conditions, aerosol concentration, and type. An aircraft prototype of the cloud scanner was built and flew in a field campaign in Brazil.

  3. Physics of Phase Space Matching for Staging Plasma and Traditional Accelerator Components Using Longitudinally Tailored Plasma Profiles.

    PubMed

    Xu, X L; Hua, J F; Wu, Y P; Zhang, C J; Li, F; Wan, Y; Pai, C-H; Lu, W; An, W; Yu, P; Hogan, M J; Joshi, C; Mori, W B

    2016-03-25

    Phase space matching between two plasma-based accelerator (PBA) stages and between a PBA and a traditional accelerator component is a critical issue for emittance preservation. The drastic differences of the transverse focusing strengths as the beam propagates between stages and components may lead to a catastrophic emittance growth even when there is a small energy spread. We propose using the linear focusing forces from nonlinear wakes in longitudinally tailored plasma density profiles to control phase space matching between sections with negligible emittance growth. Several profiles are considered and theoretical analysis and particle-in-cell simulations show how these structures may work in four different scenarios. Good agreement between theory and simulation is obtained, and it is found that the adiabatic approximation misses important physics even for long profiles. PMID:27058082

  4. Physics of Phase Space Matching for Staging Plasma and Traditional Accelerator Components Using Longitudinally Tailored Plasma Profiles

    NASA Astrophysics Data System (ADS)

    Xu, X. L.; Hua, J. F.; Wu, Y. P.; Zhang, C. J.; Li, F.; Wan, Y.; Pai, C.-H.; Lu, W.; An, W.; Yu, P.; Hogan, M. J.; Joshi, C.; Mori, W. B.

    2016-03-01

    Phase space matching between two plasma-based accelerator (PBA) stages and between a PBA and a traditional accelerator component is a critical issue for emittance preservation. The drastic differences of the transverse focusing strengths as the beam propagates between stages and components may lead to a catastrophic emittance growth even when there is a small energy spread. We propose using the linear focusing forces from nonlinear wakes in longitudinally tailored plasma density profiles to control phase space matching between sections with negligible emittance growth. Several profiles are considered and theoretical analysis and particle-in-cell simulations show how these structures may work in four different scenarios. Good agreement between theory and simulation is obtained, and it is found that the adiabatic approximation misses important physics even for long profiles.

  5. Design and analysis of a low-loss linear analog phase modulator for deep space spacecraft X-band transponder applications

    NASA Technical Reports Server (NTRS)

    Mysoor, N. R.; Mueller, R. O.

    1991-01-01

    This article summarizes the design concepts, analyses, and development of an X-band (8145 MHz) transponder low-loss linear phase modulator for deep space spacecraft applications. A single-section breadboard circulator-coupled reflection phase modulator has been analyzed, fabricated, and evaluated. A linear phase deviation of 92 deg with a linearity tolerance of +/- 8 percent was measured for this modulator from 8257 MHz to 8634 MHz over the temperature range -20 to 75 C. The measured insertion loss and the static delay variation with temperature were 2 +/- 0.3 dB and 0.16 psec/ C, respectively. Based on this design, cascaded sections have been modeled, and simulations were performed to provide an X-band deep space transponder (DST) phase modulator with +/- 2.5 radians (+/- 143 deg) of peak phase deviation to accommodate downlink signal modulation with composite telemetry data and ranging, with a deviation linearity tolerance of +/- 8 percent and insertion loss of less than 10 +/- 0.5 dB. A two-section phase modulator using constant gamma hyperabrupt varactors and an efficient modulator driver circuit was breadboarded. The measured results satisfy the DST phase-modulator requirements and show excellent agreement with the predicted results.

  6. Structure of northeastern New Mexico from deep seismic reflection profiles: Implications for the Proterozoic tectonic evolution of southwestern North America

    NASA Astrophysics Data System (ADS)

    Eshete, Tefera Gashu

    2001-09-01

    Previous geologic, geochronologic, structural, isotope, and xenolith studies have shown that the Precambrian rocks of northern New Mexico belong to the Yavapai and Mazatzal provinces. The boundary between the provinces is a wide zone defined on its northern edge by the northern extent of 1.65 Ga deformation and southern edge by the southern most extent of Yavapai crust (pre-1.7 Ga). However, the nature of the Precambrian province boundary at depth, its evolution through time, and the tectonic processes that affected the interior of these provinces, are not well understood. In order to obtain new information concerning these problems, processing and interpretation of reflection seismic data was conducted on data collected during the 1999 Continental Dynamics-Rocky Mountain (CD-ROM) project and data obtained from industry. In this study I present new information on the crustal structure of northern New Mexico provided by processing and interpretation of three seismic reflection profiles (NM-1, TB-1 and TB-2).The seismic data present evidence for Precambrian crustal growth and amalgamation, followed by subsequent reactivation of Precambrian structures. A seismic profile and gravity modeling across the NM-1 show a strongly reflective high-density (2850 kg-m-3) dome-shaped body in the middle to lower crust. On the basis of the absence of a hanging-wall antiform, the occurrence of normal sense of deflection of reflectors in the footwall, possibly Moho pullup, and geological information such as an exposed Proterozoic extensional shear zone in the Sandia Mountains, this feature is interpreted to represent a 1.4 Ga? extensional shear zone which resulted in rotation of ˜1.65 Ga imbricate thrust zones. Layered reflectivity directly below the top of Precambrian basement on profiles TB-1 and the eastern part of TB-2, based on geophysical and geological information from nearby areas is interpreted as a sequence of ˜1.4 Ga volcanic and sedimentary rocks within the Proterozoic

  7. 30 CFR 203.31 - If I have a qualified phase 2 or qualified phase 3 ultra-deep well, what royalty relief would...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... production: (1) An original well, 35 BCF. (2) A sidetrack with a sidetrack measured depth of at least 20,000... . . . Then your lease earns an RSV on this volume of gas production: (i) An original well or a sidetrack with... which is 25,000 feet TVD SS, and your lease has had no prior production from a deep or ultra-deep...

  8. 30 CFR 203.31 - If I have a qualified phase 2 or qualified phase 3 ultra-deep well, what royalty relief would...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... that is: Then your lease earns an RSV on this volume of gas production: (1) An original well, 35 BCF... RSV on this volume of gas production: (i) An original well or a sidetrack with a sidetrack measured... your lease has had no prior production from a deep or ultra-deep well. Assuming your lease has...

  9. 30 CFR 203.31 - If I have a qualified phase 2 or qualified phase 3 ultra-deep well, what royalty relief would...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of gas production: (1) An original well, 35 BCF. (2) A sidetrack with a sidetrack measured depth of... ultra-deep well that is . . Then your lease earns an RSV on this volume of gas production: (i) An... meets the condition in § 203.30(b)) of no production from a deep well. However, any remaining RSV...

  10. 30 CFR 203.31 - If I have a qualified phase 2 or qualified phase 3 ultra-deep well, what royalty relief would...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... production: (1) An original well, 35 BCF. (2) A sidetrack with a sidetrack measured depth of at least 20,000... . . . Then your lease earns an RSV on this volume of gas production: (i) An original well or a sidetrack with... which is 25,000 feet TVD SS, and your lease has had no prior production from a deep or ultra-deep...

  11. miRNA expression profiling of Epstein-Barr virus-associated NKTL cell lines by Illumina deep sequencing.

    PubMed

    Alles, Julia; Menegatti, Jennifer; Motsch, Natalie; Hart, Martin; Eichner, Norbert; Reinhardt, Richard; Meister, Gunter; Grässer, Friedrich A

    2016-04-01

    The aim of this work was to establish the microRNA profile of SNK6 and SNT16, two Epstein-Barr virus (EBV)-infected cell lines derived from nasal NK/T-cell lymphoma (NKTL). The oncogenic EBV is strongly associated with the pathogenesis of nasal and extranodal NK/T-cell lymphoma and expresses 44 mature microRNAs and two noncoding EBV-encoded RNAs (EBERs). miRNAs are 19-25nt noncoding RNAs that affect host and viral gene expression post-transcriptionally. Deregulated miRNA patterns are frequently linked to a variety of human cancers including lymphomas. miRNA profiling of the two NK/T cell lines vs. primary cells revealed 10 and 4 up-regulated and 10 and 12 down-regulated miRNAs in SNK6 and SNT16 cells respectively. The results were validated by qRT-PCR for selected miRNAs. Target gene analyses confirmed cullin 5 (CUL5) and sphingosin-1-phosphate receptor 1 (S1PR1) as targets for the down-regulated hsa-miR-148a and viral ebv-miR-BART16 respectively. As recently demonstrated for the regulation of IL1-alpha by miR-142-3p, coexpression of the EBERs selectively exerted corepression of S1PR1 by BART16 but not of CUL5 by miR-148a, indicating selective corepression by the EBERs. PMID:27239439

  12. MiRNA expression profile of ionizing radiation-induced liver injury in mouse using deep sequencing.

    PubMed

    Lu, Jike; Chen, Chen; Hao, Limin; Zheng, Zhiqiang; Zhang, Naixun; Wang, Zhenyu

    2016-08-01

    In order to investigate the potential regulatory roles of microRNAs (miRNAs) in mouse response to ionizing radiation (IR), the small RNA libraries from liver tissues of mice with or without ionizing radiation (IR) were sequenced by high-throughput deep sequencing technology. A total of 270 miRNAs including 212 known and 58 potentially novel miRNAs were identified. Within these miRNAs, there were 48 miRNAs that were differentially expressed, including 27 known and 21 novel miRNAs. The results of quantitative RT-polymerase chain reaction (qRT-PCR) were in consistent with the sequencing analysis. Target gene prediction, function annotation, and pathway of the identified miRNAs were analyzed using RNAhybrid, miRanda software and Swiss-Prot, Gene Ontology (GO), Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes, and Genomes (KEGG) and non-redundant (NR) databases. These results should be useful to investigate the biological function of miRNAs under IR-induced liver injury. PMID:27214643

  13. Magnetic graphene oxide modified with choline chloride-based deep eutectic solvent for the solid-phase extraction of protein.

    PubMed

    Huang, Yanhua; Wang, Yuzhi; Pan, Qi; Wang, Ying; Ding, Xueqin; Xu, Kaijia; Li, Na; Wen, Qian

    2015-06-01

    Four kinds of green deep eutectic solvents (DESs) based on choline chloride (ChCl) have been synthesized and coated on the surface of magnetic graphene oxide (Fe3O4@GO) to form Fe3O4@GO-DES for the magnetic solid-phase extraction of protein. X-ray diffraction (XRD), vibrating sample magnetometer (VSM), Fourier transform infrared spectrometry (FTIR), field emission scanning electron microscopy (FESEM) and thermal gravimetric analysis (TGA) were employed to characterize Fe3O4@GO-DES, and the results indicated the successful preparation of Fe3O4@GO-DES. The UV-vis spectrophotometer was used to measure the concentration of protein after extraction. Single factor experiments proved that the extraction amount was influenced by the types of DESs, solution temperature, solution ionic strength, extraction time, protein concentration and the amount of Fe3O4@GO-DES. Comparison of Fe3O4@GO and Fe3O4@GO-DES was carried out by extracting bovine serum albumin, ovalbumin, bovine hemoglobin and lysozyme. The experimental results showed that the proposed Fe3O4@GO-DES performs better than Fe3O4@GO in the extraction of acidic protein. Desorption of protein was carried out by eluting the solid extractant with 0.005 mol L(-1) Na2HPO4 contained 1 mol L(-1) NaCl. The obtained elution efficiency was about 90.9%. Attributed to the convenient magnetic separation, the solid extractant could be easily recycled. PMID:26002214

  14. Deep structure and historical earthquakes in the Calabrian subduction zone (Southern Italy): preliminary results from multi-channel seismic reflection profiles

    NASA Astrophysics Data System (ADS)

    Gallais, F.; Gutscher, M.; Torelli, L.; Polonia, A.; Riminucci, F.

    2009-12-01

    The Calabrian subduction zone is located in the complex Central Mediterranean area. This subduction is characterized by the presence of deep earthquakes under the Tyrrhenian Sea down to 500 km depth. The Tethyan remnant Ionian slab descends towards the NW at a dip of about 70° and is associated with an active volcanic arc (the Aeolian Islands). Recently reported GPS and seismicity studies suggest that the subduction of the Ionian lithosphere beneath the Calabrian Arc may be locally still active, though at very slow rates (<5 mm/yr). Moreover the offshore Calabrian accretionary wedge is known to include compressional anticlines and ongoing hydrological activity (mud volcanoes), evidence of an active deformation in the wedge. In the Calabria - Eastern Sicily area, significant historical seismicity is documented, with the strongest event occurring in 1693. The 1693 earthquake (MCS intensities = XI) destroyed the coast of Eastern Sicily (60000 people killed) and generated a 5-10 m high tsunami. Because of the tsunami generated and because the isoseismals are open to the sea, the source region appears to be offshore. The subduction fault plane would then be a good candidate for the 1693 event. Historical reports indicates that the 1169 earthquake had similar intensities and a comparable isoseismal pattern, suggesting the events may have the same source. However, a lack of instrumentally recorded thrust earthquakes, characteristic of active subduction zone, suggests that if subduction is active, the fault plane may be locked since the instrumental period. To seek evidence of continuous tectonic activity of the Calabrian system, we present preliminary results from reprocessed 96-channels seismic reflection profiles (French Archimede cruise, 1997) offshore Sicily. This analysis permits to recognize a well-defined stratigraphy in the Ionian Abyssal Plain, this stratigraphy becomes difficult to follow under the deformed Calabrian Prism. But the joint interpretation with the

  15. A crustal model of the ultrahigh-pressure Dabie Shan orogenic belt, China, derived from deep seismic refraction profiling

    USGS Publications Warehouse

    Wang, Chun-Yong; Zeng, Rong-Sheng; Mooney, W.D.; Hacker, B.R.

    2000-01-01

    We present a new crustal cross section through the east-west trending ultrahigh-pressure (UHP) Dabie Shan orogenic belt, east central China, based on a 400-km-long seismic refraction profile. Data from our profile reveal that the cratonal blocks north and south of the orogen are composed of 35-km-thick crust consisting of three layers (upper, middle, and lower crust) with average seismic velocities of 6.0±0.2 km/s, 6.5±0.1 km/s, and 6.8±0.1 km/s. The crust reaches a maximum thickness of 41.5 km beneath the northern margin of the orogen, and thus the present-day root beneath the orogen is only 6.5 km thick. The upper mantle velocity is 8.0±0.1 km/s. Modeling of shear wave data indicate that Poisson's ratio increases from 0.24±0.02 in the upper crust to 0.27±0.03 in the lower crust. This result is consistent with a dominantly felsic upper crustal composition and a mafic lower crustal composition within the amphibolite or granulite metamorphic facies. Our seismic model indicates that eclogite, which is abundant in surface exposures within the orogen, is not a volumetrically significant component in the middle or lower crust. Much of the Triassic structure associated with the formation of the UHP rocks of the Dabie Shan has been obscured by post-Triassic igneous activity, extension and large-offset strike-slip faulting. Nevertheless, we can identify a high-velocity (6.3 km/s) zone in the upper (<5 km depth) crustal core of the orogen which we interpret as a zone of ultrahigh-pressure rocks, a north dipping suture, and an apparent Moho offset that marks a likely active strike-slip fault.

  16. Double transmission-mediums based geometric phase analysis for determining the two surface profiles of transparent object

    NASA Astrophysics Data System (ADS)

    Liu, Shuli; Liu, Zhanwei

    2016-05-01

    The accurate measurement for the surface profiles of transparent object is of significance for quality control in optical devices and precision instruments. Here, a double transmission-mediums based geometric phase analysis method has been developed to evaluate both the upper and lower surface profiles of transparent object. To do this, the tested transparent object is placed above a preprinted lattice pattern. When viewed from above with a CCD camera, any slope variations of the surfaces will lead to distortions of the transmission-lattice patterns. And when changing one side of object's contact medium, the lattice virtual image with modulated phase is distorted once again. Combined with the derived relationship between phase variations of transmission-lattice patterns and out-of-plane heights of two surfaces, the double-sided surface profiles of transparent object can be reconstructed successfully. With this, the technique, which is verified experimentally, is demonstrated to be a feasible and reliable method. The advantage of this method is that it simplifies the setup and allows a fast estimation of the geometry of a transparent specimen. The double-sided profiles can be decoupled easily according to the big difference of refractive indexes between contact mediums. And the calculation accuracy can be guaranteed by the weighted average from four directions.

  17. Deep Sequencing of the Scutellaria baicalensis Georgi Transcriptome Reveals Flavonoid Biosynthetic Profiling and Organ-Specific Gene Expression

    PubMed Central

    Liu, Jinxin; Hou, Jingyi; Jiang, Chao; Li, Geng; Lu, Heng; Meng, Fanyun; Shi, Linchun

    2015-01-01

    Scutellaria baicalensis Georgi has long been used in traditional medicine to treat various such widely varying diseases and has been listed in the Chinese Pharmacopeia, the Japanese Pharmacopeia, the Korean Pharmacopoeia and the European Pharmacopoeia. Flavonoids, especially wogonin, wogonoside, baicalin, and baicalein, are its main functional ingredients with various pharmacological activities. Although pharmaological studies for these flavonoid components have been well conducted, the molecular mechanism of their biosynthesis remains unclear in S. baicalensis. In this study, Illumina/Solexa deep sequencing generated more than 91 million paired-end reads and 49,507 unigenes from S. baicalensis roots, stems, leaves and flowers. More than 70% unigenes were annotated in at least one of the five public databases and 13,627 unigenes were assigned to 3,810 KEGG genes involved in 579 different pathways. 54 unigenes that encode 12 key enzymes involved in the pathway of flavonoid biosynthesis were discovered. One baicalinase and three baicalein 7-O-glucuronosyltransferases genes potentially involved in the transformation between baicalin/wogonoside and baicalein/wogonin were identified. Four candidate 6-hydroxylase genes for the formation of baicalin/baicalein and one candidate 8-O-methyltransferase gene for the biosynthesis of wogonoside/wogonin were also recognized. Our results further support the conclusion that, in S. baicalensis, 3,5,7-trihydroxyflavone was the precursor of the four above compounds. Then, the differential expression models and simple sequence repeats associated with these genes were carefully analyzed. All of these results not only enrich the gene resource but also benefit research into the molecular genetics and functional genomics in S. baicalensis. PMID:26317778

  18. Insertional mutagenesis and deep profiling reveals gene hierarchies and a Myc/p53-dependent bottleneck in lymphomagenesis.

    PubMed

    Huser, Camille A; Gilroy, Kathryn L; de Ridder, Jeroen; Kilbey, Anna; Borland, Gillian; Mackay, Nancy; Jenkins, Alma; Bell, Margaret; Herzyk, Pawel; van der Weyden, Louise; Adams, David J; Rust, Alistair G; Cameron, Ewan; Neil, James C

    2014-02-01

    Retroviral insertional mutagenesis (RIM) is a powerful tool for cancer genomics that was combined in this study with deep sequencing (RIM/DS) to facilitate a comprehensive analysis of lymphoma progression. Transgenic mice expressing two potent collaborating oncogenes in the germ line (CD2-MYC, -Runx2) develop rapid onset tumours that can be accelerated and rendered polyclonal by neonatal Moloney murine leukaemia virus (MoMLV) infection. RIM/DS analysis of 28 polyclonal lymphomas identified 771 common insertion sites (CISs) defining a 'progression network' that encompassed a remarkably large fraction of known MoMLV target genes, with further strong indications of oncogenic selection above the background of MoMLV integration preference. Progression driven by RIM was characterised as a Darwinian process of clonal competition engaging proliferation control networks downstream of cytokine and T-cell receptor signalling. Enhancer mode activation accounted for the most efficiently selected CIS target genes, including Ccr7 as the most prominent of a set of chemokine receptors driving paracrine growth stimulation and lymphoma dissemination. Another large target gene subset including candidate tumour suppressors was disrupted by intragenic insertions. A second RIM/DS screen comparing lymphomas of wild-type and parental transgenics showed that CD2-MYC tumours are virtually dependent on activation of Runx family genes in strong preference to other potent Myc collaborating genes (Gfi1, Notch1). Ikzf1 was identified as a novel collaborating gene for Runx2 and illustrated the interface between integration preference and oncogenic selection. Lymphoma target genes for MoMLV can be classified into (a) a small set of master regulators that confer self-renewal; overcoming p53 and other failsafe pathways and (b) a large group of progression genes that control autonomous proliferation in transformed cells. These findings provide insights into retroviral biology, human cancer genetics

  19. Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression

    PubMed Central

    2012-01-01

    Background Clostridium thermocellum produces H2 and ethanol, as well as CO2, acetate, formate, and lactate, directly from cellulosic biomass. It is therefore an attractive model for biofuel production via consolidated bioprocessing. Optimization of end-product yields and titres is crucial for making biofuel production economically feasible. Relative protein expression profiles may provide targets for metabolic engineering, while understanding changes in protein expression and metabolism in response to carbon limitation, pH, and growth phase may aid in reactor optimization. We performed shotgun 2D-HPLC-MS/MS on closed-batch cellobiose-grown exponential phase C. thermocellum cell-free extracts to determine relative protein expression profiles of core metabolic proteins involved carbohydrate utilization, energy conservation, and end-product synthesis. iTRAQ (isobaric tag for relative and absolute quantitation) based protein quantitation was used to determine changes in core metabolic proteins in response to growth phase. Results Relative abundance profiles revealed differential levels of putative enzymes capable of catalyzing parallel pathways. The majority of proteins involved in pyruvate catabolism and end-product synthesis were detected with high abundance, with the exception of aldehyde dehydrogenase, ferredoxin-dependent Ech-type [NiFe]-hydrogenase, and RNF-type NADH:ferredoxin oxidoreductase. Using 4-plex 2D-HPLC-MS/MS, 24% of the 144 core metabolism proteins detected demonstrated moderate changes in expression during transition from exponential to stationary phase. Notably, proteins involved in pyruvate synthesis decreased in stationary phase, whereas proteins involved in glycogen metabolism, pyruvate catabolism, and end-product synthesis increased in stationary phase. Several proteins that may directly dictate end-product synthesis patterns, including pyruvate:ferredoxin oxidoreductases, alcohol dehydrogenases, and a putative bifurcating hydrogenase

  20. Analysis of black pepper volatiles by solid phase microextraction-gas chromatography: A comparison of terpenes profiles with hydrodistillation.

    PubMed

    Jeleń, Henryk H; Gracka, Anna

    2015-10-30

    Solid phase microextraction (SPME) is widely used in food flavor compounds analysis in majority for profiling volatile compounds. Based on such profiles conclusions are often drawn concerning the percentage composition of volatile compounds in particular food, spices or raw materials. This paper focuses on the usefulness of SPME for the profiling of volatile compounds from spices using black pepper as an example. SPME profiles obtained in different analytical conditions were compared to the profile of pepper volatiles obtained using hydrodistillation in Clevenger apparatus. The profiles of both monoterpenes and sesquiterpenes of black pepper were highly dependent on sample weight (0.1 and 1g samples were tested), and extraction time (durations from 2 to 120min were tested), regardless of the SPME fiber used (PDMS and CAR/PDMS coatings were used). The characteristic phenomenon for extraction from dry ground pepper was the decrease of monoterpenes % share in volatiles with increasing extraction times, whereas at the same time the % contents of sesquiterpenes increased. Addition of water to ground pepper substantially changed extraction kinetics and mutual proportions of mono to sesquiterpenes compared to dry samples by minimizing changes in mono- to sesquiterpenes ratio in different extraction times. Obtained results indicate that SPME can be a fast extraction method for volatiles of black pepper. Short extraction times (2-10min) in conjunction with the fast GC analysis (2.1min) proposed here may offer fast alternative to hydrodistillation allowing black pepper terpenes characterization. PMID:26427328

  1. Transcriptome profiling and digital gene expression by deep sequencing in early somatic embryogenesis of endangered medicinal Eleutherococcus senticosus Maxim.

    PubMed

    Tao, Lei; Zhao, Yue; Wu, Ying; Wang, Qiuyu; Yuan, Hongmei; Zhao, Lijuan; Guo, Wendong; You, Xiangling

    2016-03-01

    Somatic embryogenesis (SE) has been studied as a model system to understand molecular events in physiology, biochemistry, and cytology during plant embryo development. In particular, it is exceedingly difficult to access the morphological and early regulatory events in zygotic embryos. To understand the molecular mechanisms regulating early SE in Eleutherococcus senticosus Maxim., we used high-throughput RNA-Seq technology to investigate its transcriptome. We obtained 58,327,688 reads, which were assembled into 75,803 unique unigenes. To better understand their functions, the unigenes were annotated using the Clusters of Orthologous Groups, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases. Digital gene expression libraries revealed differences in gene expression profiles at different developmental stages (embryogenic callus, yellow embryogenic callus, global embryo). We obtained a sequencing depth of >5.6 million tags per sample and identified many differentially expressed genes at various stages of SE. The initiation of SE affected gene expression in many KEGG pathways, but predominantly that in metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction. This information on the changes in the multiple pathways related to SE induction in E. senticosus Maxim. embryogenic tissue will contribute to a more comprehensive understanding of the mechanisms involved in early SE. Additionally, the differentially expressed genes may act as molecular markers and could play very important roles in the early stage of SE. The results are a comprehensive molecular biology resource for investigating SE of E. senticosus Maxim. PMID:26657036

  2. Comparison of phase velocities from array measurements of Rayleigh waves associated with microtremor and results calculated from borehole shear-wave velocity profiles

    USGS Publications Warehouse

    Liu, Hsi-Ping; Boore, David M.; Joyner, William B.; Oppenheimer, David H.; Warrick, Richard E.; Zhang, Wenbo; Hamilton, John C.; Brown, Leo T.

    2000-01-01

    Shear-wave velocities (VS) are widely used for earthquake ground-motion site characterization. VS data are now largely obtained using borehole methods. Drilling holes, however, is expensive. Nonintrusive surface methods are inexpensive for obtaining VS information, but not many comparisons with direct borehole measurements have been published. Because different assumptions are used in data interpretation of each surface method and public safety is involved in site characterization for engineering structures, it is important to validate the surface methods by additional comparisons with borehole measurements. We compare results obtained from a particular surface method (array measurement of surface waves associated with microtremor) with results obtained from borehole methods. Using a 10-element nested-triangular array of 100-m aperture, we measured surface-wave phase velocities at two California sites, Garner Valley near Hemet and Hollister Municipal Airport. The Garner Valley site is located at an ancient lake bed where water-saturated sediment overlies decomposed granite on top of granite bedrock. Our array was deployed at a location where seismic velocities had been determined to a depth of 500 m by borehole methods. At Hollister, where the near-surface sediment consists of clay, sand, and gravel, we determined phase velocities using an array located close to a 60-m deep borehole where downhole velocity logs already exist. Because we want to assess the measurements uncomplicated by uncertainties introduced by the inversion process, we compare our phase-velocity results with the borehole VS depth profile by calculating fundamental-mode Rayleigh-wave phase velocities from an earth model constructed from the borehole data. For wavelengths less than ~2 times of the array aperture at Garner Valley, phase-velocity results from array measurements agree with the calculated Rayleigh-wave velocities to better than 11%. Measurement errors become larger for wavelengths 2

  3. Crustal structure and Moho geometry of the northeastern Tibetan plateau as revealed by SinoProbe-02 deep seismic-reflection profiling

    NASA Astrophysics Data System (ADS)

    Wang, Haiyan; Gao, Rui; Zeng, Lingsen; Kuang, Zhaoyang; Xue, Aimin; Li, Wenhui; Xiong, Xiaosong; Huang, Weiyi

    2014-12-01

    Underthrusting of India below Eurasia has resulted in the formation of the Himalayan and the Qinghai-Tibet Plateau. Distributed deformation coupled with block translation and rotation has generated the Qilian Shan thrust belt and a series of east- and northwest-striking strike-slip faults across northeastern Tibet. Because these structures lie in a transition zone between the high plateau region to the south and the lowlands of the North China craton in the north, determining their deep-crustal and upper-mantle structures has important implications for unraveling the mechanism of Tibetan plateau formation. In this paper, we present new results from the SinoProbe-02 deep seismic reflection project across the eastern part of the Qilian Shan and the southern margin of the Alxa block. Interpretation of the reflection profile obtained from this study is based on constraints from surface geology and detailed geometric analysis of structural relationships among key reflectors in the crust and the upper mantle. Our results indicate that the upper crust of the eastern Qilian Shan is characterized by fault-bend folds and duplex systems involving Phanerozoic strata that may have resulted from early Paleozoic collisional tectonics and Cenozoic intra-continental deformation. Locally, half-graben structures hosting Cretaceous strata are also present. The active structures in the region are dominated by left-slip Haiyuan and Tianjian fault systems marking the northern margin of the Tibetan plateau. The strike-slip structures have variable dips and dip directions and sole into a common décollement with a depth of 40-45 km. Because the two faults do not cut and offset the Moho below, the active crustal and mantle deformation in the northeastern Tibet must be decoupled.

  4. National Environmental/Energy Workforce Assessment, Phase II, Post-Secondary Education Profile: Pesticides.

    ERIC Educational Resources Information Center

    National Field Research Center Inc., Iowa City, IA.

    Pesticide- and toxicology-related programs were reviewed in 21 states and in 26 academic institutions. These programs represent a sample, only, of the various programs available nationwide. Enrollment profiles are given for both pesticide and toxicology programs. The programs described in these profiles are served by a total of 620 faculty.…

  5. Expression Profiling of Preadipocyte MicroRNAs by Deep Sequencing on Chicken Lines Divergently Selected for Abdominal Fatness

    PubMed Central

    Wang, Weishi; Du, Zhi-Qiang; Cheng, Bohan; Wang, Yuxiang; Yao, Jing; Li, Yumao; Cao, Zhiping; Luan, Peng; Wang, Ning; Li, Hui

    2015-01-01

    Through posttranscriptional gene regulation, microRNA (miRNA) is linked to a wide variety of biological processes, including adipogenesis and lipid metabolism. Although miRNAs in mammalian adipogenesis have been worked on extensively, their study in chicken adipogenesis is still very limited. To find miRNAs potentially important for chicken preadipocyte development, we compared the preadipocyte miRNA expression profiles in two broiler lines divergently selected for abdominal fat content, by sequencing two small RNA libraries constructed for primary preadipocytes isolated from abdominal adipose tissues. After bioinformatics analyses, from chicken miRNAs deposited in miRBase 20.0, we identified 225 miRNAs to be expressed in preadipocytes, 185 in the lean line and 200 in the fat line (derived from 208 and 203 miRNA precursors, respectively), which corresponds to 114 miRNA families. The let-7 family miRNAs were the most abundant. Furthermore, we validated the sequencing results of 15 known miRNAs by qRT-PCR, and confirmed that the expression levels of most miRNAs correlated well with those of Solexa sequencing. A total of 33 miRNAs was significantly differentially expressed between the two chicken lines (P<0.05). Gene ontology analysis revealed that they could target genes enriched in the regulation of gene transcription and chromatin function, response to insulin stimulation, and IGF-1 signaling pathways, which could have important roles in preadipocyte development. Therefore, a valuable information and resource of miRNAs on chicken adipogenesis were provided in this study. Future functional investigations on these miRNAs could help explore related genes and molecular networks fundamental to preadipocyte development. PMID:25675096

  6. Effects of self-heating and phase change on the thermal profile of hydrogen isotopes in confined geometries

    SciTech Connect

    Baxamusa, S. Field, J.; Dylla-Spears, R.; Kozioziemski, B.; Suratwala, T.; Sater, J.

    2014-03-28

    Growth of high-quality single-crystal hydrogen in confined geometries relies on the in situ formation of seed crystals. Generation of deuterium-tritium seed crystals in a confined geometry is governed by three effects: self-heating due to tritium decay, external thermal environment, and latent heat of phase change at the boundary between hydrogen liquid and vapor. A detailed computation of the temperature profile for liquid hydrogen inside a hollow shell, as is found in inertial confinement fusion research, shows that seeds are likely to form at the equatorial plane of the shell. Radioactive decay of tritium to helium slowly alters the composition of the hydrogen vapor, resulting in a modified temperature profile that encourages seed formation at the top of the shell. We show that the computed temperature profile is consistent with a variety of experimental observations.

  7. Layer Stripping Forward Tomography with S, ScS, and Sdiff phases to sharpen images of deep mantle shear velocity heterogeneity

    NASA Astrophysics Data System (ADS)

    Lai, H.; Garnero, E.; Zhao, C.

    2014-12-01

    The Earth's deep mantle has been shown to be heterogeneous at a wide spectrum of scales, from fine scale (e.g.,1-10 km) to degree 2 low velocity provinces (1000's of km lateral scale). Principle structures include large low shear velocity provinces (LLSVPs) beneath the Pacific Ocean and Africa and southern Atlanic Ocean, and a circum-Pacific higher velocity band. At intermediate scales, structures include D" discontinuities and details associated with LLSVPs, such as sharp sides. Shorter scales include ultra-low velocity zones (ULVZs) and regions of scattering of high frequency waves. In this study we seek to improve upon our knowledge of global intermediate and small-scale shear wave structure in the lower mantle with an iterative Layer-Stripping Forward Tomography approach. This approach iteratively updates a starting tomographic model by mapping travel time residuals (between observations and predictions) from the surface (using shallow S wave phase) down to lowermost mantle depths (using deep S waves, ScS waves, and Sdiff waves). Modifications to the uppermost mantle are primarily aimed at properly correcting deep phases for shallow heterogeneity. Relatively uniform coverage of the deepest mantle is achieved with Sdiff. The core-reflected ScS contributes most strongly to our knowledge of shorter scale heterogeneity, but coverage is more limited that with Sdiff. Deep mantle S waves provide information on the vertical extent of lowermost mantle heterogeneity (e.g., LLSVPs), but similar to ScS, coverage is limited to source-receiver pairs with the proper distance range. We collected near 1200 global earthquakes global earthquakes from the last two decades, with depths greater than 30 km, and focused on data with clear and impulsive phases. In a semi-automatic scheme, we measured all S, ScS, and Sdiff times and document waveshape stability by constructing a mean shape of each phase for each event. Cross-correlation coefficients between individual records and the

  8. Soil Organic Matter Characterization by 13C-NMR and Thermal Analysis in Deep Tropical Soil Profiles from the Luquillo Critical Zone Observatory

    NASA Astrophysics Data System (ADS)

    Plante, A. F.; Hockaday, W. C.

    2015-12-01

    Tropical forest soils store large quantities of carbon (C) as soil organic matter (SOM), a substantial proportion of which is stored deep (> 30 cm) in the soil profile. Characterization of tropical SOM remains difficult, in part due to the analytical challenges associated high iron and low C concentrations. In this study, we combined solid-state 13C nuclear magnetic resonance (NMR) spectroscopy with analytical thermal analysis (differential scanning calorimetry, DSC; evolved CO2 gas analysis, CO2-EGA) to explore patterns in SOM composition in deep soil profiles from two contrasting soil types at the Luquillo Critical Zone Observatory (LCZO) in northeast Puerto Rico. Prior to 13C NMR, soils were repeatedly demineralized with hydrofluoric acid (HF) to remove paramagnetic compounds and concentrate organic matter. Given the scant information on tropical subsoil OM, we also sought to evaluate the effect of HF acid treatments on tropical subsoil SOM. HF treatments effectively enriched sample C and removed paramagnetic compounds, allowing us to obtain high-quality NMR spectra for low-C subsoils. C:N ratios before and after HF treatment were nearly identical (mean = 16.6 ± 0.8), suggesting that the SOM pool was not substantially fractionated, though C recoveries were low and variable. Thermal analyses confirmed the loss of a substantial fraction of the soil mineral matrix, however, retention of several endothermic regions in post-HF Inceptisol soils indicated that not all minerals were completely solubilized. In addition, important differences in the DSC and CO2-EGA thermograms were observed in comparing samples before versus after HF treatments. These results suggest that the organo-mineral associations were substantially altered, though it is not immediately clear the degree to which alterations in chemical composition versus binding association have changed. In addition to these qualitative changes, quantitative interpretations of 13C-NMR results from low-C and high

  9. Joint pre-stack depth migration and travel-time tomography applied to a deep seismic profile across the northern Barents Sea igneous province

    NASA Astrophysics Data System (ADS)

    Minakov, Alexander; Faleide, Jan Inge; Sakulina, Tamara; Krupnova, Natalia; Dergunov, Nikolai

    2015-04-01

    The mainly Permo-Triassic North Barents Sea Basin is considered as a superdeep intracratonic basin containing over 20 km of sedimentary material. This basin was strongly affected by magmatism attributed to the formation of the Early Cretaceous High Arctic Large Igneous Province. Dolerite dikes, sills, and lava flows are observed in the northern Barents Sea and on the islands of Svalbard and Franz Josef Land. Some dike swarms can be traced over hundreds of kilometers using high-resolution airborne magnetic data. In the North Barents Sea Basin, the dikes fed giant sill complex emplaced into organic-rich Triassic siliciclastic rocks. The sill complex creates a major challenge for seismic imaging masking the underlying strata. In this contribution, we first perform refraction and reflection travel-time tomography using wide-angle ocean-bottom seismometer data (with receivers deployed every 10 km) along the 4-AR profile (Sakulina et al. 2007, Ivanova et al. 2011). The resulting tomographic model is then used to construct a background velocity model for the pre-stack depth migration. We show that the use of a combined velocity model for the time and depth imaging based on travel-time tomography and RMS velocities constitutes a substantial improvement with respect to a standard processing workflow providing a more coherent seismic structure of this volcanic province. The interpretation of multichannel seismic and high-resolution magnetic data together with P-wave velocity and density anomalies allow to create a model for the system of magmatic feeders in the crystalline basement of the northern Barents Sea region. Sakulina, T.S., Verba, M.L., Ivanova, N.M., Krupnova, N.A., Belyaev I.V., 2007. Deep structure of the north Barents-Kara Region along 4AR transect (Taimyr Peninsula - Franz Joseph Land). In: Models of the Earth's crust and upper mantle after deep seismic profiling. Proceedings of the international scientific-practical seminar. Rosnedra, VSEGEI. St

  10. Discovery and profiling of novel and conserved microRNAs during flower development in Carya cathayensis via deep sequencing.

    PubMed

    Wang, Zheng Jia; Huang, Jian Qin; Huang, You Jun; Li, Zheng; Zheng, Bing Song

    2012-08-01

    Hickory (Carya cathayensis Sarg.) is an economically important woody plant in China, but its long juvenile phase delays yield. MicroRNAs (miRNAs) are critical regulators of genes and important for normal plant development and physiology, including flower development. We used Solexa technology to sequence two small RNA libraries from two floral differentiation stages in hickory to identify miRNAs related to flower development. We identified 39 conserved miRNA sequences from 114 loci belonging to 23 families as well as two novel and ten potential novel miRNAs belonging to nine families. Moreover, 35 conserved miRNA*s and two novel miRNA*s were detected. Twenty miRNA sequences from 49 loci belonging to 11 families were differentially expressed; all were up-regulated at the later stage of flower development in hickory. Quantitative real-time PCR of 12 conserved miRNA sequences, five novel miRNA families, and two novel miRNA*s validated that all were expressed during hickory flower development, and the expression patterns were similar to those detected with Solexa sequencing. Finally, a total of 146 targets of the novel and conserved miRNAs were predicted. This study identified a diverse set of miRNAs that were closely related to hickory flower development and that could help in plant floral induction. PMID:22481137

  11. Widely tunable broadband deep-ultraviolet to visible wavelength generation by the cross phase modulation in a hollow-core photonic crystal fiber cladding

    NASA Astrophysics Data System (ADS)

    Yuan, J. H.; Sang, X. Z.; Wu, Q.; Yu, C. X.; Zhou, G. Y.; Shen, X. W.; Wang, K. R.; Yan, B. B.; Teng, Y. L.; Xia, C. M.; Han, Y.; Li, S. G.; Farrell, G.; Hou, L. T.

    2013-08-01

    The deep-ultraviolet (UV) to visible wavelengths are efficiently generated for the first time by the cross phase modulation (XPM) between the red-shifted solitons and the blue-shifted dispersive waves (DWs) in the fundamental guided mode of the multi-knots of a hollow-core photonic crystal fiber cladding (HC-PCFC). When the femtosecond pulses with a wavelength of 850 nm and average power of 300 mW are coupled into the knots 1-3, the conversion efficiency ηuv-v of 11% and bandwidth Buv-v of 100 nm in the deep-UV region are experimentally obtained. The multi-milliwatt ultrashort pulses are tunable over the deep-UV (below 200 nm) to visible spectral region by adjusting the wavelengths of the pump pulses in different knots. It is expected that these widely tunable broadband ultrashort deep-UV-visible pulse sources could have important applications in ultrafast photonics, femtochemisty, photobiology, and UV-visible resonant Raman scattering.

  12. Deep sequencing-based transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus reveals insight into the immune-relevant genes in marine fish

    PubMed Central

    2010-01-01

    Background Systematic research on fish immunogenetics is indispensable in understanding the origin and evolution of immune systems. This has long been a challenging task because of the limited number of deep sequencing technologies and genome backgrounds of non-model fish available. The newly developed Solexa/Illumina RNA-seq and Digital gene expression (DGE) are high-throughput sequencing approaches and are powerful tools for genomic studies at the transcriptome level. This study reports the transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus using RNA-seq and DGE in an attempt to gain insights into the immunogenetics of marine fish. Results RNA-seq analysis generated 169,950 non-redundant consensus sequences, among which 48,987 functional transcripts with complete or various length encoding regions were identified. More than 52% of these transcripts are possibly involved in approximately 219 known metabolic or signalling pathways, while 2,673 transcripts were associated with immune-relevant genes. In addition, approximately 8% of the transcripts appeared to be fish-specific genes that have never been described before. DGE analysis revealed that the host transcriptome profile of Vibrio harveyi-challenged L. japonicus is considerably altered, as indicated by the significant up- or down-regulation of 1,224 strong infection-responsive transcripts. Results indicated an overall conservation of the components and transcriptome alterations underlying innate and adaptive immunity in fish and other vertebrate models. Analysis suggested the acquisition of numerous fish-specific immune system components during early vertebrate evolution. Conclusion This study provided a global survey of host defence gene activities against bacterial challenge in a non-model marine fish. Results can contribute to the in-depth study of candidate genes in marine fish immunity, and help improve current understanding of host-pathogen interactions and evolutionary history

  13. Haemato-biochemical and endocrine profiling of north western Himalayan Gaddi sheep during various physiological/reproductive phases

    PubMed Central

    Sharma, A.; Kumar, P.; Singh, M.; Vasishta, N.K.

    2015-01-01

    The study was aimed to provide baseline data regarding haemato-biochemical and endocrine profiling of Gaddi sheep found in north western Himalayan region of Himachal Pradesh, India. Each random sample was collected from 45 Gaddi sheep reared in government sheep breeding farm Tal, Hamirpur, India, during various reproductive phases viz. anestrus, breeding season and post partum period. Haematology revealed significantly higher (P<0.05) RBC as well as haematocrit values in pregnant animals (n=23) during breeding season than during other reproductive phases. The number of platelets were significantly lower (P<0.05) and MCH, MCHC values were statistically higher (P<0.05) during postpartum period than during other reproductive phases. Blood biochemistry revealed significantly higher (P<0.05) concentrations of plasma cholesterol (83.98±3.68 mg/dl), plasma calcium (71.06±1.52 mg/l), magnesium (18.21±0.53 mg/l), potassium (5.10±0.13 mEq/l) and significantly lower (P<0.05) concentrations of plasma total protein (5.75±0.31 gm/dl), globulin (3.04±0.29 gm/dl) and sodium (138.83±1.83 mEq/l) during postpartum period in comparison to other reproductive phases. Endocrine profile revealed significantly higher (P<0.05) serum estrogen (60.97±1.24 pg/ml) and T4 (6.0±0.27 µg/ml) concentrations during postpartum phase. Similarly, significantly higher (P<0.05) serum progesterone (5.16±0.76 ng/ml) as well as TSH (0.70±0.14 µg/ml) concentration were recorded during pregnancy. From the study it can be concluded that physiological status significantly affects the blood metabolic and endocrine profile in Gaddi sheep. PMID:26623374

  14. Haemato-biochemical and endocrine profiling of north western Himalayan Gaddi sheep during various physiological/reproductive phases.

    PubMed

    Sharma, A; Kumar, P; Singh, M; Vasishta, N K

    2015-01-01

    The study was aimed to provide baseline data regarding haemato-biochemical and endocrine profiling of Gaddi sheep found in north western Himalayan region of Himachal Pradesh, India. Each random sample was collected from 45 Gaddi sheep reared in government sheep breeding farm Tal, Hamirpur, India, during various reproductive phases viz. anestrus, breeding season and post partum period. Haematology revealed significantly higher (P<0.05) RBC as well as haematocrit values in pregnant animals (n=23) during breeding season than during other reproductive phases. The number of platelets were significantly lower (P<0.05) and MCH, MCHC values were statistically higher (P<0.05) during postpartum period than during other reproductive phases. Blood biochemistry revealed significantly higher (P<0.05) concentrations of plasma cholesterol (83.98±3.68 mg/dl), plasma calcium (71.06±1.52 mg/l), magnesium (18.21±0.53 mg/l), potassium (5.10±0.13 mEq/l) and significantly lower (P<0.05) concentrations of plasma total protein (5.75±0.31 gm/dl), globulin (3.04±0.29 gm/dl) and sodium (138.83±1.83 mEq/l) during postpartum period in comparison to other reproductive phases. Endocrine profile revealed significantly higher (P<0.05) serum estrogen (60.97±1.24 pg/ml) and T4 (6.0±0.27 µg/ml) concentrations during postpartum phase. Similarly, significantly higher (P<0.05) serum progesterone (5.16±0.76 ng/ml) as well as TSH (0.70±0.14 µg/ml) concentration were recorded during pregnancy. From the study it can be concluded that physiological status significantly affects the blood metabolic and endocrine profile in Gaddi sheep. PMID:26623374

  15. Modeling and Observations of Phase-Mask Trapezoidal Profiles with Grating-Fiber Image Reproduction

    NASA Technical Reports Server (NTRS)

    Lyons, Donald R.; Lindesay, James V.; Lee, Hyung R.; Ndlela, Zolili U.; Thompso, Erica J.

    2000-01-01

    We report on an investigation of the trapezoidal design and fabrication defects in phase masks used to produce Bragg reflection gratings in optical fibers. We used a direct visualization technique to examine the nonuniformity of the interference patterns generated by several phase masks. Fringe patterns from the phase masks are compared with the analogous patterns resulting from two-beam interference. Atomic force microscope imaging of the actual phase gratings that give rise to anomalous fringe patterns is used to determine input parameters for a general theoretical model. Phase masks with pitches of 0.566 and 1.059 microns are modeled and investigated.

  16. Continuation of a deep borehole stress measurement profile near the San Andreas Fault: 1. Hydraulic fracturing stress measurements at Hi Vista, Mojave Desert, California

    NASA Astrophysics Data System (ADS)

    Hickman, Stephen H.; Zoback, Mark D.; Healy, John H.

    1988-12-01

    Hydraulic fracturing stress measurements were made in a 592-m-deep well at Hi Vista, California, 32 km from the San Andreas fault in the western Mojave Desert. The relative magnitudes of the horizontal principal stresses and the calculated overburden stress indicate that the stress regime at this site is transitional between thrust faulting and strike-slip faulting. The azimuths of the induced hydraulic fractures at Hi Vista exhibit considerable scatter, and the indicated direction of the maximum horizontal principal stress ranges from north-northeast to northwest. The measured magnitudes of the horizontal principal stresses and the horizontal deviatoric stress in this well are less than or equal to those measured in a nearby well of comparable depth 4 km from the San Andreas fault. This result contrasts with the increase in these stress components with distance from the San Andreas fault that was observed in a shallower borehole profile in the same area. Marked fluctuations in both stress magnitudes and orientations with depth in the Hi Vista well, however, may result from a localized perturbation to the regional stress regime. No correlation was found to exist in this well between stress magnitudes and either P wave velocities or natural fracture densities, although the low stresses measured at a depth of about 540 m may reflect proximity to an intensely fractured and permeable zone at the bottom of the well.

  17. The utility of diversity profiling using Illumina 18S rRNA gene amplicon deep sequencing to detect and discriminate Toxoplasma gondii among the cyst-forming coccidia.

    PubMed

    Cooper, Madalyn K; Phalen, David N; Donahoe, Shannon L; Rose, Karrie; Šlapeta, Jan

    2016-01-30

    Next-generation sequencing (NGS) has the capacity to screen a single DNA sample and detect pathogen DNA from thousands of host DNA sequence reads, making it a versatile and informative tool for investigation of pathogens in diseased animals. The technique is effective and labor saving in the initial identification of pathogens, and will complement conventional diagnostic tests to associate the candidate pathogen with a disease process. In this report, we investigated the utility of the diversity profiling NGS approach using Illumina small subunit ribosomal RNA (18S rRNA) gene amplicon deep sequencing to detect Toxoplasma gondii in previously confirmed cases of toxoplasmosis. We then tested the diagnostic approach with species-specific PCR genotyping, histopathology and immunohistochemistry of toxoplasmosis in a Risso's dolphin (Grampus griseus) to systematically characterise the disease and associate causality. We show that the Euk7A/Euk570R primer set targeting the V1-V3 hypervariable region of the 18S rRNA gene can be used as a species-specific assay for cyst-forming coccidia and discriminate T. gondii. Overall, the approach is cost-effective and improves diagnostic decision support by narrowing the differential diagnosis list with more certainty than was previously possible. Furthermore, it supplements the limitations of cryptic protozoan morphology and surpasses the need for species-specific PCR primer combinations. PMID:26801593

  18. Dividing phase-dependent cytotoxicity profiling of human embryonic lung fibroblast identifies candidate anticancer reagents.

    PubMed

    Inagaki, Yoshinori; Matsumoto, Yasuhiko; Tang, Wei; Sekimizu, Kazuhisa

    2016-01-01

    Human Embryonic Lung fibroblasts (HEL cells) are widely used as a normal cell in studies of cell biology and can be easily maintained in the resting phase. Here we aimed to discover compounds that exhibit cytotoxicity against HEL cells in the dividing phase, but not in the resting phase. The cytotoxicity of each compound against HEL cells either in the resting phase or in the dividing phase was determined by MTT assay. Ratios of the IC50 of cells in the resting phase and that of cells in the dividing phase (RRD) for these compounds were compared. We selected 44 compounds that exhibited toxic effects on HEL cells in the dividing phase from a chemical library containing 325 anticancer drugs and enzyme inhibitors. The RRD values of those compounds were widely distributed. Paclitaxel and docetaxel, which are clinically used as anticancer drugs, had RRD values larger than 2000. On the other hand, the RRD value of dimethyl sulfoxide, an organic solvent, was 1. The cytotoxic effect of paclitaxel on HEL cells in the dividing phase was attenuated by aphidicolin, hydroxyurea, and nocodazole, confirming that the cytotoxic effects of paclitaxel are dependent on cells being in the dividing phase. Thapsigargin, whose RRD value was 800, the third highest RRD value in the library, exhibited therapeutic effects in a mouse model of FM3A ascites carcinoma. We suggest that compounds with high RRD values for HEL cells are candidate anticancer chemotherapy seeds. PMID:27594296

  19. Geophysical constraints on the crustal structure of the East European Platform margin and its foreland based on the POLCRUST-01 deep reflection seismic profile

    NASA Astrophysics Data System (ADS)

    Malinowski, M.; Guterch, A.; Narkiewicz, M.; Petecki, Z.; Janik, T.; Środa, P.; Maksym, A.; Probulski, J.; Grad, M.; Czuba, W.; Gaczyński, E.; Majdański, M.; Jankowski, L.

    2015-06-01

    A new 240-km long, deep seismic reflection profile (POLCRUST-01) was recently acquired in SE Poland crossing the East European Platform (EEP) margin south-east of the North-German-Polish Caledonides (NGPC). Here we document geophysical field work and subsequent data processing and modeling. Results obtained from reflection seismic data are augmented by results of the first-arrival tomography applied to co-located extended-offset refraction data, as well as potential field modeling and comparison with the available wide-angle reflection/refraction data. Our preferred model of the crustal structure, derived by integrating seismic, potential field and geological data, is composed of crustal blocks (terranes) separated by nearly-vertical faults. These are: (I) intact part of the EEP; (II) Łysogóry Terrane; (III) Małopolska Terrane; and (IV) Carpathian Mts. with their basement. Reflective lower crust of the EEP can be an inherited feature of crustal extension (rifting) or compressional tectonics acting at the cratonic margin. The Teisseyre-Tornquist Zone (TTZ) is depicted as a Caledonian transcurrent accretion zone corresponding with the near-vertical Tomaszów Fault, bounding the Łysogóry Terrane to the east. The crust of the Łysogóry Terrane suggests EEP affinity, although its middle/lower crust thickness is highly reduced. The Małopolska Terrane seems to be internally subdivided into blocks of different magnetic properties of the lower crust. The Carpathian frontal thrust is associated with a change in the rock properties in the deep basement (an unknown crustal block?) which is not visible in seismic data alone. The interpreted structure of the Caledonian terranes and their tectonic boundaries favors a transcurrent style of a crustal accretion along the central and SE Polish segments of the TTZ, implying a very complex nature of the Caledonian accretionary belt of Central Europe: from an array of terranes displaced along the TTZ to an accretionary wedge of

  20. Estimating production and consumption of solid reactive Fe phases in marine sediments from concentration profiles

    EPA Science Inventory

    1D diffusion models may be used to estimate rates of production and consumption of dissolved metabolites in marine sediments, but are applied less often to the solid phase. Here we used a numerical inverse method to estimate solid phase Fe(III) and Fe(II) consumption and product...

  1. National Environmental/Energy Workforce Assessment, Phase II, Post-Secondary Education Profile: Noise.

    ERIC Educational Resources Information Center

    National Field Research Center Inc., Iowa City, IA.

    Noise-related training programs were reviewed in nine degree-granting institutions in seven states. These programs represent a sample, only, of the various programs available nationwide. The enrollment profile and average number of graduates by degree level for the past three years are given, as well as the apparent enrollment trends by degree…

  2. Deep geometry and evolution of the northern part of Itoigwa-Shizuoka Tectonic Line active fault system, Central Japan, revealed by Seismic profiling

    NASA Astrophysics Data System (ADS)

    Sato, H.; Ikeda, Y.; Iwasaki, T.; Matsuta, N.; Takeda, T.; Kawasaki, S.; Kozawa, T.; Elouai, D.; Hirata, N.; Kawanaka, T.

    2003-12-01

    The northern Fossa Magna (NFM) is a Miocene rift system produced in the final stages of the opening of the Sea of Japan. It divides the major structure of Japan into SW and NE portions. The Itoigawa-Shizuoka Tectonic Line (ISTL) bounds the western part of the northern Fossa Magna and forms an active fault system showing the one of the largest slip rates in the Japanese islands. Based on the paleo-seismological data, the ISTL active fault system was evaluated to have the highest seismic risk among active faults within inland Japan. A quantitative understanding of active tectonic processes, including crustal deformation and related destructive earthquakes, is important in reducing seismic hazards through precise estimation of strong ground motions. The structure of the crust, especially the deep geometry of active fault systems, is the most important piece information required to construct such a dynamic model. In this context, the seismic reflection profiling was performed across the northern part of the ISTL active fault system by three seismic lines. Obtained seismic sections are interpreted based on the pattern of reflectors, surface geology and velocity model by refraction analysis, using the balanced cross section technique. The 68-km-long Itoshizu 2002 seismic section across the northern middle part of the ISTL active fault system suggest that the Miocene NFM basin was formed by an east dipping normal fault with shallow flat (6 km), deeper ramp (6 15 km) and deeper flat at 15 km in depth. This unique geometry is interpreted that this low-angle normal fault was produced by Miocene high thermal regime, estimated from the thick volcanic rocks at the base of the basin fill. Namely, the normal fault reflects the brittle-ductile boundary in Miocene. Consequently, since the Pliocene, the basin fill was strongly folded by the reverse faulting along the pre-existing normal faults in the Pre-Neogene rocks. The reverse faults in the basin fill produced fault

  3. Production of mineral surface area within deep weathering profiles at eroding vs. depositional hillslope locations: Christina River Basin Critical Zone Observatory

    NASA Astrophysics Data System (ADS)

    Fisher, B.; Yoo, K.; Aufdenkampe, A. K.; Nater, E.

    2014-12-01

    Geomorphic and biogeochemical processes and hillslope morphology are partly controlled by the extent and degree of chemical weathering between soil and bedrock. The production of mineral specific surface area (SSA) via chemical weathering is a critical variable for mechanistic understanding of weathering and provides an interface between minerals and the soil carbon cycle. We examined two 21-meter deep drill cores in the Laurels Schist at 141 MASL (summit) and 130 MASL (interfluve) in a 900 ha first order watershed in the Laurels Preserve, a forested land use end member in the Christina River Basin CZO. In addition to mineral SSA, we report elemental and mineralogical changes through both weathering profiles. Despite highly variable bedrock composition, mobile elements (Ca & Na) are depleted within 3-5 m below the ground surface, which is consistent with the removal of Ca-Na-plagioclase ((Na,Ca)Al(Si,Al)3O8) at this interval; we consider this depth as a weathering front. The water table in both boreholes was ~123 MASL (5/2014), which is well below the weathering front, suggesting that weathering processes are not coupled with groundwater interactions in this system. Clay XRD reveals the presence of secondary phyllosilicates including vermiculite, illite, and kaolinite in the upper 3 m of the summit weathering profile, which are weathering products of primary plagioclase, muscovite, and chlorite. The currently available clay mineralogy results are consistent with the decrease in total SSA from up to 20 m2g-1 at the surface to <5 m2g-1 below 3 m depth. Within the first 3 m from the surface, citrate-dithionate extractable iron contributed 30-60% of the total surface area. Therefore transformation of primary minerals to secondary phyllosilicate minerals, involving leaching loss of cations, was partly responsible for SSA production, but iron oxides play a significant role in production of SSA above the weathering front. This observation did not differ between

  4. Deep brain stimulation of the subthalamic nucleus preferentially alters the translational profile of striatopallidal neurons in an animal model of Parkinson's disease

    PubMed Central

    Visanji, Naomi P.; Kamali Sarvestani, Iman; Creed, Meaghan C.; Shams Shoaei, Zahra; Nobrega, José N.; Hamani, Clement; Hazrati, Lili-Naz

    2015-01-01

    Deep brain stimulation targeting the subthalamic nucleus (STN-DBS) is an effective surgical treatment for the motor symptoms of Parkinson's disease (PD), the precise neuronal mechanisms of which both at molecular and network levels remain a topic of debate. Here we employ two transgenic mouse lines, combining translating ribosomal affinity purification (TRAP) with bacterial artificial chromosome expression (Bac), to selectively identify changes in translational gene expression in either Drd1a-expressing striatonigral or Drd2-expressing striatopallidal medium spiny neurons (MSNs) of the striatum following STN-DBS. 6-hydroxydopamine lesioned mice received either 5 days stimulation via a DBS electrode implanted in the ipsilateral STN or 5 days sham treatment (no stimulation). Striatal polyribosomal RNA was selectively purified from either Drd2 or Drd1a MSNs using the TRAP method and gene expression profiling performed. We identified eight significantly altered genes in Drd2 MSNs (Vps33b, Ppp1r3c, Mapk4, Sorcs2, Neto1, Abca1, Penk1, and Gapdh) and two overlapping genes in Drd1a MSNs (Penk1 and Ppp1r3c) implicated in the molecular mechanisms of STN-DBS. A detailed functional analysis, using a further 728 probes implicated in STN-DBS, suggested an increased ability to receive excitation (mediated by increased dendritic spines, increased calcium influx and enhanced excitatory post synaptic potentials) accompanied by processes that would hamper the initiation of action potentials, transport of neurotransmitters from soma to axon terminals and vesicular release in Drd2-expressing MSNs. Finally, changes in expression of several genes involved in apoptosis as well as cholesterol and fatty acid metabolism were also identified. This increased understanding of the molecular mechanisms induced by STN-DBS may reveal novel targets for future non-surgical therapies for PD. PMID:26106299

  5. Development of a High-Performance, Low-Profile Translation Table with Wire Feedthrough for a Deep Space CubeSat

    NASA Technical Reports Server (NTRS)

    Few, Alex

    2016-01-01

    NEAScout, a 6U cubesat and secondary payload on NASA's EM-1, will use an 85 sq m solar sail to travel to a near-earth asteroid at about 1 Astronomical Unit (about 1.5 x 10(exp 8) km) for observation and reconnaissance1. A combination of reaction wheels, reaction control system, and a slow rotisserie roll about the solar sail's normal axis were expected to handle attitude control and adjust for imperfections in the deployed sail during the 2.5-year mission. As the design for NEAScout matured, one of the critical design parameters, the offset in the center of mass and center of pressure (CP/CM offset), proved to be sub-optimal. After significant mission and control analysis, the CP/CM offset was accommodated by the addition of a new subsystem to NEAScout. This system, called the Active Mass Translator (AMT), would reside near the geometric center of NEAScout and adjust the CM by moving one portion of the flight system relative to the other. The AMT was given limited design space - 17 mm of the vehicle's assembly height-and was required to generate +/-8 cm by +/-2 cm translation to sub-millimeter accuracy. Furthermore, the design must accommodate a large wire bundle of small gage, single strand wire and coax cables fed through the center of the mechanism. The bend radius, bend resistance, and the exposure to deep space environment complicates the AMT design and operation and necessitated a unique design to mitigate risks of wire bundle damage, binding, and cold-welding during operation. This paper will outline the design constraints for the AMT, discuss the methods and reasoning for design, and identify the lessons learned through the designing, breadboarding and testing for the low-profile translation stages with wire feedthrough capability.

  6. Brasiliano crustal structure and the tectonic setting of the Parnaíba basin of NE Brazil: Results of a deep seismic reflection profile

    NASA Astrophysics Data System (ADS)

    Daly, Michael C.; Andrade, Vander; Barousse, Chuck A.; Costa, Rafaela; McDowell, Kenneth; Piggott, Neil; Poole, Alan J.

    2014-11-01

    A 1430 km, deep crustal, seismic reflection profile of the Parnaíba basin shows an asymmetric, structured western margin and a gently dipping eastern margin. The ~3 km thick, Phanerozoic sedimentary section overlies a pronounced, planar, regional unconformity that crosses three Precambrian blocks with differing seismic facies: the Amazonian/Araguaia block, the Parnaíba block, and the Borborema block. The blocks are separated by steep crustal-scale boundaries across which seismic facies change abruptly. In the west, the ophiolitic metasedimentary rocks of the Araguaia Group overlie the Amazonian craton. Both craton and metasediments terminate eastward against a steep, east dipping fault zone defining the Amazonian/Araguaia block and Parnaíba block boundary. Reactivation of this Neoproterozoic margin in the Late Triassic and Late Jurassic/Early Cretaceous, folded and elevated basement and basin over 2 km. A second crustal boundary defines the eastern margin of the Parnaíba block with the Neoproterozoic Borborema block. This boundary is interpreted as the extension of the Transbrasiliano shear zone. These data demonstrate that the basement of the Parnaíba basin was formed during Brasiliano orogenesis by west directed collision-related thrusting, succeeded by lateral accretion along steep, crustal-scale boundaries. After formation of a post-Brasiliano peneplain, the Parnaíba basin developed seamlessly across three very different crustal blocks and appears to have been significantly larger than its present outline. No extensive underlying rift system is evident suggesting that basement structure had little to do with basin formation, but that episodic reactivation of the boundary zones and basement fabric has controlled the structuring and preservation of the basin.

  7. Deep brain stimulation of the subthalamic nucleus preferentially alters the translational profile of striatopallidal neurons in an animal model of Parkinson's disease.

    PubMed

    Visanji, Naomi P; Kamali Sarvestani, Iman; Creed, Meaghan C; Shams Shoaei, Zahra; Nobrega, José N; Hamani, Clement; Hazrati, Lili-Naz

    2015-01-01

    Deep brain stimulation targeting the subthalamic nucleus (STN-DBS) is an effective surgical treatment for the motor symptoms of Parkinson's disease (PD), the precise neuronal mechanisms of which both at molecular and network levels remain a topic of debate. Here we employ two transgenic mouse lines, combining translating ribosomal affinity purification (TRAP) with bacterial artificial chromosome expression (Bac), to selectively identify changes in translational gene expression in either Drd1a-expressing striatonigral or Drd2-expressing striatopallidal medium spiny neurons (MSNs) of the striatum following STN-DBS. 6-hydroxydopamine lesioned mice received either 5 days stimulation via a DBS electrode implanted in the ipsilateral STN or 5 days sham treatment (no stimulation). Striatal polyribosomal RNA was selectively purified from either Drd2 or Drd1a MSNs using the TRAP method and gene expression profiling performed. We identified eight significantly altered genes in Drd2 MSNs (Vps33b, Ppp1r3c, Mapk4, Sorcs2, Neto1, Abca1, Penk1, and Gapdh) and two overlapping genes in Drd1a MSNs (Penk1 and Ppp1r3c) implicated in the molecular mechanisms of STN-DBS. A detailed functional analysis, using a further 728 probes implicated in STN-DBS, suggested an increased ability to receive excitation (mediated by increased dendritic spines, increased calcium influx and enhanced excitatory post synaptic potentials) accompanied by processes that would hamper the initiation of action potentials, transport of neurotransmitters from soma to axon terminals and vesicular release in Drd2-expressing MSNs. Finally, changes in expression of several genes involved in apoptosis as well as cholesterol and fatty acid metabolism were also identified. This increased understanding of the molecular mechanisms induced by STN-DBS may reveal novel targets for future non-surgical therapies for PD. PMID:26106299

  8. Comparison of chromatographic band profiles obtained under microwave irradiated and non-irradiated reversed-phase liquid chromatography column

    SciTech Connect

    Galinada, Wilmer; Guiochon, Georges A

    2005-08-01

    The possible influence of the application of microwave energy to a reversed-phase liquid chromatography column on the mass transfer kinetics and the thermodynamics of equilibrium between mobile and stationary phases was examined. Chromatograms of propylbenzene and phenol were recorded under the same experimental conditions, on the same column, successively irradiated and not. The effect of microwave irradiation on the mass transfer kinetics was determined by measuring the second moment of small pulses of propylbenzene in a 70:30 (v/v) solution of methanol in water and microwave outputs of 15 and 30 W. The effect of microwave irradiation on the equilibrium thermodynamics was determined by measuring the elution time of breakthrough curves of phenol at high concentrations in a 20:80 (v/v) solution of methanol and water and microwave outputs of 15, 50, and 150 W. A qualitative comparison of the profiles of the propylbenzene peaks obtained with and without irradiation suggests that this irradiation affects significantly the peak shapes. However, a qualitative comparison of the profiles of the breakthrough curves of phenol obtained with and without irradiation suggests that this irradiation has no significant effect on their shapes. The peak sharpening observed may be due to an increase in the diffusivity, resulting from the dielectric polarization under microwave irradiation. This effect is directly related to an increase of the rate of mass transfers in the column. In contrast, the similarity of the overloaded band profiles at high concentrations suggests that the equilibrium thermodynamics is unaffected by microwave irradiation. This may be explained by the transparence of the stationary phase to microwaves at 2.45 GHz. The column temperature was measured at the column outlet under irradiation powers of 15, 30, 50, and 150 W. It increases with increasing power, the corresponding effluent temperatures being 25 {+-} 1, 30 {+-} 1, 35 {+-} 1, and 45 {+-} 1 C, respectively.

  9. Impact of High-Frequency Spectral Phase Modulation on the Temporal Profile of Short Optical Pulses

    SciTech Connect

    Dorrer, C.; Bromage, J.

    2008-03-18

    The impact of high-frequency spectral phase modulation on the temporal intensity of optical pulses is derived analytically and simulated in two different regimes. The temporal contrast of an optical pulse close to the Fourier-transform limit is degraded by a pedestal related to the power spectral density of the spectral phase modulation. When the optical pulse is highly chirped, its intensity modulation is directly related to the spectral phase variations with a transfer function depending on the second-order dispersion of the chirped pulse. The metrology of the spectral phase of an optical pulse using temporal-intensity measurements performed after chirping the pulse is studied. The effect of spatial averaging is also discussed.

  10. Single scan defect identification by deep level transient spectroscopy using a two-phase lock-in amplifier (IQ-DLTS)

    NASA Astrophysics Data System (ADS)

    Auret, F. D.; Nel, M.

    1988-02-01

    A simple method of analyzing the deep level transient spectroscopy (DLTS) transient signal by recording its in-phase (I) and quadrature (Q) DLTS spectra using a two-phase lock-in amplifier with a sine wave mixing function, IQ-DLTS, is presented. Measurement of the peak positions on the I and Q spectra, which are simultaneously recorded during a single temperature scan, facilitates the calculation of the defect's activation energy Et, and capture cross section σt, which are required for its identification. It was found that the Et values obtained when analyzing proton implantation-induced defects in GaAs were within 5% of those determined from the conventional DLTS Arrhenius plots.

  11. CD and profile metrology of embedded phase shift masks using scatterometry

    NASA Astrophysics Data System (ADS)

    Lee, Kyung man; Yedur, Sanjay; Hetzer, Dave; Tavassoli, Malahat; Baik, Kiho

    2006-06-01

    Linewidth and etch depth control on the photomask is rapidly becoming a major concern in mask processing. In this paper, we report on a Scatterometry based metrology system that provides line width and etch profile measurements on Embedded PSMs on Intel's 65nm and 45nm node test masks. Measurements were made with Nanometric's Atlas-M reticle measurement system. Spectrum data obtained from plates were analyzed using Timbre Technologies' ODP analysis software. We characterized the CD uniformity, linearity, sidewall angle and thickness uniformity. Significant reduction in time per measurement is achieved when compared to CD-SEM. ODP Scatterometry reported a 2x reduction in the CD Uniformity compared to that reported from the SEM. This reduction is typically due to outliers reported by the CD-SEM that is averaged out in ODP Scatterometry. Good correlation to top-down CD-SEM and cross-sectional SEM is reported. R-squared correlation of >0.99 (ODP scatterometry to top down CD-SEM) is reported. Profile measurements from ODP show excellent match to cross-section SEM. The data show that Scatterometry provides a nondestructive way to monitor basic etch profile combined with relatively little CD metrology lag.

  12. Preparation of a nitro-substituted tris(indolyl)methane modified silica in deep eutectic solvents for solid-phase extraction of organic acids.

    PubMed

    Wang, Na; Wang, Jiamin; Liao, Yuan; Shao, Shijun

    2016-05-01

    A new sorbent for solid-phase extraction was synthesized by chemical immobilization of nitro-substituted tris(indolyl)methane on silica in new and green deep eutectic solvents. Elemental analysis results indicated that deep eutectic solvents could be an alternative to the traditional solvents in preparing nitro-substituted tris(indolyl)methane modified silica. Coupled with high performance liquid chromatography, the extraction performance of the sorbent was evaluated by using four organic acids as model analytes. The rebinding experiments results showed that the nitro-substituted tris(indolyl)methane modified silica sorbent had a good adsorption capacity towards the selected organic acids. Under the appropriate experimental conditions, good precision and wide linear ranges with coefficient of determination (R(2)) of higher than 0.9957 were obtained, and the limits of detection were in the range of 0.50-2.0μgL(-1) for the organic acids tested. The developed solid-phase extraction-high performance liquid chromatography-diode array detection (SPE-HPLC-DAD) method was successfully applied for the determination of organic acids in two drinking samples with recoveries ranging from 76.7% to 110.0% and 67.7% to 104.0% for all the selected organic acids, respectively. PMID:26946003

  13. Identification of multiple reflected phases from migration receiver function profile: An example for the INDEPTH-III passive teleseismic P waveform data

    NASA Astrophysics Data System (ADS)

    Tian, Xiaobo; Wu, Qingju; Zhang, Zhongjie; Teng, Jiwen; Zeng, Rongsheng

    2005-04-01

    The crustal multiple reflected phases usually interfere with those converted phases from the topmost mantle discontinuities, which make them difficult to be identified and should be carefully interpreted in a migration receiver functions profile. We propose a simple scheme to identify the crustal multiples in a migration receiver functions profile. The scheme is based on the differences in both geometric and dynamic features resolved using AVP (amplitude variation verse ray parameter) and DVP (discontinuity depth variation verse ray parameter). Synthetic data shows that the crustal multiples can be distinguished from those converted phases in the receiver function migration profile, especially when the receiver functions are migrated using velocities lower than the actual velocities. As an example, we apply our procedure to the INDEPTH-III passive teleseismic P waveform data. Our results indicate that a possible converted phase from the ``220-km'' discontinuity is a crustal multiple reflected phase.

  14. Fast processing of quantitative phase profiles from off-axis interferograms for real-time applications

    NASA Astrophysics Data System (ADS)

    Girshovitz, Pinhas; Shaked, Natan T.

    2015-03-01

    We review new and efficient algorithms, lately presented by us, for rapid reconstruction of quantitative phase maps from off-axis digital interferograms. These algorithms improve the conventional Fourier-based algorithm by using the Fourier transforms and the phase unwrapping process more efficiently, and thus decrease the calculation complexity required for extracting the sample phase map from the recorded interferograms. Using the new algorithms, on a standard personal computer without using the graphic processing-unit programming or parallel computing, we were able to speed up the processing and reach frame rates of up to 45 frames per second for one megapixel off-axis interferograms. These capabilities allow real-time visualization, calculation and data extraction for dynamic samples and processes, inspected by off-axis digital holography. Specific applications include biological cell imaging without labeling and real-time nondestructive testing.

  15. Phase-correlated P Cygni profile variations of the C III multiplet in UW Canis Majoris

    NASA Technical Reports Server (NTRS)

    Drechsel, H.; Kondo, Y.; Mccluskey, G. E., Jr.; Rahe, J.

    1981-01-01

    The interacting close binary system UW CMa has been observed, in the wavelength range from 1161 to 1188 A, continuously during a complete orbital cycle in 1979 with the Copernicus (OAO-3) U2 spectrometer. The C III multiplet at 1175 A, observed as a P Cygni feature, exhbits a clear dependence on the orbital phase of the binary; the radial velocity variation of this feature lags behind that of the O7 primary component by 0.1 orbital phase, which agrees with the anticipations in an earlier study by the same authors. The radiation-driven matter, flowing out of the binary, originates in the primary component.

  16. Method for the measurement of surface-relief grating's profile using initial phase of diffraction wave

    NASA Astrophysics Data System (ADS)

    Feng, Fanrong; Wu, Jianhong; Gao, Fei

    2015-11-01

    The analysis of initial phase of diffraction wave of grating mask is based on rigorous coupled-wave analysis method. In this paper, the general diffraction analysis numerical code based on the rigorous coupled-wave analysis (RCWA) is written by MATLAB software to calculate the 0th refraction wave of grating mask. Since large measurement errors will occur while measuring the grove shape by AFM, the method of measuring the initial phase of diffraction wave was proposed and the feasibility of this method has also been verified.

  17. Profiling of soluble neutral oligosaccharides from treated biomass using solid phase extraction and LC-TOF MS.

    PubMed

    Vismeh, Ramin; Humpula, James F; Chundawat, Shishir P S; Balan, Venkatesh; Dale, Bruce E; Jones, A Daniel

    2013-05-15

    Thermochemical pretreatments of cellulosic biomass are known to improve cell wall enzymatic digestibility, while simultaneously releasing substantial amounts of soluble oligosaccharides. Profiling of oligosaccharides released during pretreatment yields information essential for choosing glycosyl hydrolases necessary for cost-effective conversion of cellulosic biomass to desired biofuel/biochemical end-products. In this report we present a methodology for profiling of soluble neutral oligosaccharides released from ammonia fiber expansion (AFEX™)-pretreated corn stover. Our methodology employs solid phase extraction (SPE) enrichment of oligosaccharides using porous graphitized carbon (PGC), followed by high performance liquid chromatography (HPLC) separation using a polymeric amine based column and electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS). For structural elucidation on the chromatographic time scale, nonselective multiplexed collision-induced dissociation was performed for quasi-simultaneous acquisition of oligosaccharide molecular and fragment masses in a single analysis. These analyses revealed glucans up to degree of polymerization (DP) 22 without modifications. Additionally, arabinoxylans up to DP=6 were detected in pretreated biomass extracts (post-enzymatic digestion). Cross-ring fragment ion abundances were consistent with assignment of linkages between sugar units in glucans and also xylose backbone in arabinoxylans as 1-4 linkages. Comprehensive profiling of soluble oligosaccharides also demonstrated decreases in levels of acetate esters of arabinoxylan oligosaccharides with concomitant increases in nonacetylated oligosaccharides that were consistent with earlier observations of 85% release of acetate esters by AFEX™ pretreatment. PMID:23544634

  18. Tradeoffs in Chemical and Thermal Variations in the Post-perovskite Phase Transition: Mixed phase regions in the Deep Lower Mantle?

    SciTech Connect

    Frank J Spera; David A. Yuen; Grace Giles

    2007-04-01

    The discovery of a phase-transition in Mg-rich perovskite (Pv) to a post-perovskite (pPv) phase at lower mantle depths and its relationship to D", lower mantle heterogeneity and iron content prompted an investigation of the relative importance of lower mantle compositional and temperature fluctuations in creating topographic undulations on mixed phase regions. Above the transition, Mg-rich Pv makes up ~ 70 per cent by mass of the lower mantle. Using results from experimental phase equilibria, first-principles computations and empirical scaling relations for Fe2+-Mg mixing in silicates, a preliminary thermodynamic model for the Pv to pPv phase transition in the divariant system MgSiO3-FeSiO3 is developed. Complexities associated with components Fe2O3 and Al2O3 and other phases (Ca-Pv, magnesiowustite) are neglected. The model predicts phase transition pressures are sensitive to the FeSiO3 content of perovskite (~ -1.5 GPa per one mole percent FeSiO3). This leads to considerable topography along the top boundary of the mixed phase region. The Clapeyron slope for the Pv→pPv transition at XFeSiO3= 0.1 is +11 MPa/K about 20% higher than for pure Mg-Pv. Increasing bulk concentration of iron elevates the mixed (two-phase) layer above the core–mantle boundary (CMB); increasing temperature acts to push the mixed layer deeper in the lower mantle perhaps into the D” thermal-compositional boundary layer resting upon the CMB. For various lower mantle geotherms and CMB temperatures, a single mixed layer of thickness ~300 km lies within the bottom 40% of the lower mantle. For low iron contents (XFeSiO3 ~ 5 mole percent or less), two (perched) mixed phase layers are found. This is the divariant analog to the univariant double-crosser of Hernlund, et al., 2005. The hotter the mantle, the deeper the mixed phase layer; the more iron-rich the lower mantle, the shallower the mixed phase layer. In a younger and hotter Hadean Earth with interior temperatures everywhere 200-500 K

  19. A Phase III, Randomized, Multi-Center, Double-Masked, Matched-Pairs, Active-Controlled Trial to Compare the Efficacy and Safety between Neuramis Deep and Restylane in the Correction of Nasolabial Folds

    PubMed Central

    Pak, Changsik; Park, Jihoon; Hong, Jinmyung; Jeong, Jaehoon; Bang, Saik

    2015-01-01

    Background We conducted this clinical study to compare the efficacy and safety between Neuramis Deep and Restylane in the correction of nasolabial folds. Methods In this phase III, randomized, multi-center, double-masked, matched-pairs, active-controlled trial (ClinicalTrials.gov Identifier: NCT01585220), we evaluated a total of 67 subjects (n=67). All the subjects underwent Neuramis Deep treatment on one side and Restylane on the contralateral side of the bilateral nasolabial folds at a ratio of 1:1. To compare the efficacy of Neuramis Deep and Restylane, we evaluated the Wrinkle Severity Rating Scale scores and those of the Global Aesthetic Improvement Scale. In addition, we compared the safety of Neuramis Deep and Restylane based on adverse events, physical examination, and clinical laboratory tests. Results Neuramis Deep was not inferior in improving the nasolabial folds as compared with Restylane. In addition, there was no significant difference in the efficacy between Neuramis Deep and Restylane. There were no significant differences in safety parameters between Neuramis Deep and Restylane. Conclusions In conclusion, our results indicate that Neuramis Deep may be a safe, effective material for improving the nasolabial folds. However, further studies are warranted to compare the tolerability of Neuramis Deep and Restylane based on histopathologic findings. PMID:26618119

  20. Profile of the bovine acute-phase response following an intravenous bolus-dose lipopolysaccharide challenge

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two experiments were conducted to further define the acute-phase response to a lipopolysaccharide (LPS) challenge in beef steers. In Exp. 1, 9 crossbred beef steers (449 ± 12 kg BW) were used in a completely random design to determine the effects of 0.5, 1.0, or 2.0 micrograms of LPS/kilogram of bod...

  1. Comparison of ISO Standard and TKR Patient Axial Force Profiles during the Stance Phase of Gait

    PubMed Central

    Lundberg, Hannah J.; Ngai, Valentina; Wimmer, Markus A.

    2012-01-01

    Preclinical endurance testing of total knee replacements (TKRs) is performed using International Organization for Standardization (ISO) load and motion protocols. The standards are based on data from normal subjects and may not sufficiently mimic in vivo implant conditions. In this study, a mathematical model was used to calculate the axial force profile of 30 TKR patients with two current implant types, 22 with NexGen and eight with Miller-Galante II Cruciate-Retaining TKRs, and statistically compare the axial force specified by the ISO standard to the TKR patients. Significant differences were found between the axial forces of both groups of TKR patients and the ISO standard at local maxima and minima points in the first half of stance. The force impulse (area under the axial force curve, representing cumulative loading) was smaller for the ISO standard than the TKR patients, but only for those with NexGen implants. Waveform analysis using the coefficient of multiple correlation showed that the ISO and TKR patient axial force profiles were similar. The combined effect of ISO standard compressive load and motion differences from TKR patients could explain some of the differences between the wear scars on retrieved tibial components and those tested in total joint simulators. PMID:22558837

  2. Testing the limits of the dune phase-stability diagram: the influence of velocity profile shape on dune morphology

    NASA Astrophysics Data System (ADS)

    Unsworth, C. A.; Parsons, D. R.; Reesink, A. J. H.; McLelland, S.

    2014-12-01

    The past 100 years of research on fluvial dunes and their deposits has produced bedform scaling laws based on flow depth, grain size and flow velocity. Such flow-form-deposit scaling is used ubiquitously for a wide range of paleo-environmental interpretations and in predictions of river bed roughness in floods. Recent research from marine environments, density currents, and fluvial flows with strong secondary circulation shows that these laws are often extrapolated beyond the limits of the original research. In submarine density currents, for example, paleo-hydraulic reconstructions commonly predict dune forming flow conditions, but preserved dune cross strata are rarely found. One particular difference between these geophysical flows is the velocity profile shape and bed shear stress that results. In a series of novel laboratory experiments the shape of the mean downstream velocity profile was systematically altered so the velocity maximum was lowered toward the bed through the addition of roughness elements at the water surface; whilst maintaining flow depth and depth-averaged velocities. This produced velocity profile shapes closer to those in density currents, and open-channel flows with strong secondary circulation. The initial lowering of the velocity maximum position increased dune height and length by 250%. The lowest velocity maximum position produced a stable upper-stage plane bed, whilst predictions based on flow depth and mean velocity remained within the dune regime phase-space. The results therefore demonstrate that the vertical position of the downstream velocity maximum can be a better predictor of equilibrium bedform geometries than flow depth or depth averaged velocity and also highlight that paleo-hydraulic reconstructions need to account for the possible variation in profile shape between geophysical flows. This research improves fundamental understanding of fluvial bedform stability and flow-form-deposit scaling laws for extrapolation into in a

  3. A Universal Power-law Profile of Pseudo-phase-space Density-like Quantities in Elliptical Galaxies

    NASA Astrophysics Data System (ADS)

    Chae, Kyu-Hyun

    2014-06-01

    We study profiles of mass density, velocity dispersion (VD), and a combination of both using ~2000 nearly spherical and rotation-free Sloan Digital Sky Survey galaxies. For observational stellar mass density ρsstarf(r), we consider a range of dark matter (DM) distribution ρDM(r) and VD anisotropy β(r) to investigate radial stellar VD σsstarfr(r) using the spherical Jeans equation. While mass and VD profiles vary appreciably depending on DM distribution and anisotropy, the pseudo-phase-space density-like combination \\rho (r)/\\sigma _\\star r^3(r) with total density ρ(r) = ρsstarf(r) + ρDM(r) is nearly universal. In the optical region, the negative logarithmic slope has a mean value of langχrang ≈ 1.86-1.90 with a galaxy-to-galaxy rms scatter of ≈0.04-0.06, which is a few times smaller than that of ρ(r) profiles. The scatter of χ can be increased by invoking wildly varying anisotropies that are, however, less likely because they would produce too large a scatter of line of sight VD profiles. As an independent check of this universality, we analyze stellar orbit-based dynamical models of 15 early-type galaxies (ETGs) of the Coma cluster provided by J. Thomas. Coma ETGs, with σsstarfr(r) replaced by the rms velocity of stars v sstarfrms(r) including net rotation, exhibit a similar universality with a slope of χ = 1.93 ± 0.06. Remarkably, the inferred values of χ for ETGs match well the slope ≈1.9 predicted by N-body simulations of DM halos. We argue that the inferred universal nature of \\rho (r)/\\sigma _\\star r^3(r) cannot be fully explained by equilibrium alone, implying that some astrophysical factors conspire and/or it reflects a fundamental principle in collisionless formation processes.

  4. In Vivo Profiling and Distribution of Known and Novel Phase I and Phase II Metabolites of Efavirenz in Plasma, Urine, and Cerebrospinal Fluid.

    PubMed

    Aouri, Manel; Barcelo, Catalina; Ternon, Béatrice; Cavassini, Matthias; Anagnostopoulos, Alexia; Yerly, Sabine; Hugues, Henry; Vernazza, Pietro; Günthard, Huldrych F; Buclin, Thierry; Telenti, Amalio; Rotger, Margalida; Decosterd, Laurent A

    2016-01-01

    Efavirenz (EFV) is principally metabolized by CYP2B6 to 8-hydroxy-efavirenz (8OH-EFV) and to a lesser extent by CYP2A6 to 7-hydroxy-efavirenz (7OH-EFV). So far, most metabolite profile analyses have been restricted to 8OH-EFV, 7OH-EFV, and EFV-N-glucuronide, even though these metabolites represent a minor percentage of EFV metabolites present in vivo. We have performed a quantitative phase I and II metabolite profile analysis by tandem mass spectrometry of plasma, cerebrospinal fluid (CSF), and urine samples in 71 human immunodeficiency virus patients taking efavirenz, prior to and after enzymatic (glucuronidase and sulfatase) hydrolysis. We have shown that phase II metabolites constitute the major part of the known circulating efavirenz species in humans. The 8OH-EFV-glucuronide (gln) and 8OH-EFV-sulfate (identified for the first time) in humans were found to be 64- and 7-fold higher than the parent 8OH-EFV, respectively. In individuals (n = 67) genotyped for CYP2B6, 2A6, and CYP3A metabolic pathways, 8OH-EFV/EFV ratios in plasma were an index of CYP2B6 phenotypic activity (P < 0.0001), which was also reflected by phase II metabolites 8OH-EFV-glucuronide/EFV and 8OH-EFV-sulfate/EFV ratios. Neither EFV nor 8OH-EFV, nor any other considered metabolites in plasma were associated with an increased risk of central nervous system (CNS) toxicity. In CSF, 8OH-EFV levels were not influenced by CYP2B6 genotypes and did not predict CNS toxicity. The phase II metabolites 8OH-EFV-gln, 8OH-EFV-sulfate, and 7OH-EFV-gln were present in CSF at 2- to 9-fold higher concentrations than 8OH-EFV. The potential contribution of known and previously unreported EFV metabolites in CSF to the neuropsychological effects of efavirenz needs to be further examined in larger cohort studies. PMID:26553012

  5. Deep Recycling of Sedimentary Lithologies in Subduction Zones: Geochemical and Physical Constraints from Phase Equilibria and Synchrotron-Based Multi-Anvil Experiments at 15-25 GPa

    NASA Astrophysics Data System (ADS)

    Rapp, R. P.; Nishiyama, N.; Irifune, T.; Inoue, T.; Yamasaki, D.

    2003-12-01

    Ocean island basalts (OIBs) provide geochemical evidence for the presence of crustally-derived sedimentary material in the deep mantle plume source region for EM-type OIBs, and global seismic tomography provides us with dramatic images of subducted slabs, presumably carrying a sediment component, penetrating through the transition zone and into the lower mantle, in some cases to the core-mantle boundary. In an effort to better constrain the geochemical effects of deeply recycled sedimentary material in subduction zones, and their role in the petrogenesis of EM-type OIBs, we have undertaken a series of phase equlibria experiments in the multi-anvil apparatus at 10-25 GPa, using natural sediment lithologies as starting materials. The goal of these experiments is to identify the dominant phases in deeply subducted sediments, constrain their P-T stability limits, and to assess their role in crustal recycling and element redistribution in the deep mantle during subduction. The phase equilibria experiments were performed in a 2000-ton Kawai-type apparatus, using tungsten carbide cubes with 3 mm TEL and Cr-doped MgO and zirconia pressure media. A cylindrical lanthanum chromite heater was used, along with short (< 1 mm), thick-walled, pressure-welded gold capsules to minimize thermal gradients and to retain the small amounts of water (< 1 wt%) present in the starting material, and long run-durations (12-48 hours) in order to facilitate future analyses of the dominant phases for key trace elements using the ion microprobe. Our preliminary results at 10-25 GPa indicate that K-hollandite (KalSi3O3) and stishovite are the primary high-pressure phases in the sediment composition, with subordinate garnet and an as-yet-unidentified (possibly hydrous) Al-silicate phase present as well. These results suggest that K-hollandite is the primary repository for incompatible elements (e.g., La, Ce, Sr, Ba, Rb, etc., and the heat-producing elements K, U and Th) in sedimentary material

  6. Optical gain, phase shift, and profile in free-electron lasers. Interim report

    SciTech Connect

    Hafizi, B.; Sprangle, P.A.; Ting, A.

    1987-07-20

    The gain, phase shift, wavefront curvature and radius of the radiation envelope in a free-electron-laser amplifier are obtained in the small signal regime. The electron beam is assumed to have a Gaussian density distribution in the transverse direction. Numerical calculations indicate that the radius and curvature of the radiation beam entering a wiggler asymptote have unique, spatially constant values after a finite transition region. However, in the asymptotic region, the wavefronts are divergent. Analytical expressions for the gain, phase shift, curvature, and spot size are derived. It is shown analytically that small perturbations of the optical waist and curvature about the matched value are spatially damped out, indicating the stability of the matched envelope. When the electron beam envelope is modulated in space, the optical spot size oscillates with an almost identical wavelength but is delayed in phase. In the case of small-amplitude long-wavelength betatron modulation of the electron-beam envelope, generation of optical sidebands in wave-number space is examined, and the effect on the dispersions characteristics of the primary wave is found to be negligible for typical experimental parameters.

  7. B1 mapping with a pure phase encode approach: Quantitative density profiling

    NASA Astrophysics Data System (ADS)

    Vashaee, S.; Newling, B.; MacMillan, B.; Balcom, B. J.

    2013-07-01

    In MRI, it is frequently observed that naturally uniform samples do not have uniform image intensities. In many cases this non-uniform image intensity is due to an inhomogeneous B1 field. The ‘principle of reciprocity' states that the received signal is proportional to the local magnitude of the applied B1 field per unit current. Inhomogeneity in the B1 field results in signal intensity variations that limit the ability of MRI to yield quantitative information. In this paper a novel method is described for mapping B1 inhomogeneities based on measurement of the B1 field employing centric-scan pure phase encode MRI measurements. The resultant B1 map may be employed to correct related non-uniformities in MR images. The new method is based on acquiring successive images with systematically incremented low flip angle excitation pulses. The local image intensity variation is proportional to B12, which ensures high sensitivity to B1 field variations. Pure phase encoding ensures the resultant B1 field maps are free from distortions caused by susceptibility variation, chemical shift and paramagnetic impurities. Hence, the method works well in regions of space that are not accessible to other methods such as in the vicinity of conductive metallic structures, such as the RF probe itself. Quantitative density images result when the centric scan pure phase encode measurement is corrected with a relative or absolute B1 field map. The new technique is simple, reliable and robust.

  8. Wheat digalactosyldiacylglycerol molecular species profiling using porous graphitic carbon stationary phase.

    PubMed

    Deschamps, Frantz S; Gaudin, Karen; Baillet, Arlette; Chaminade, Pierre

    2004-11-01

    The potential of porous graphitic carbon stationary phase (PGC) was assessed for the separation of molecular species of digalactosyldiacylglycerol (DGDG). Detection was by an evaporative light scattering detector (ELSD). A conventional optimization strategy allowed definition of a quaternary non-aqueous mobile phase and separation of 9 wheat DGDG molecular species with isocratic elution: methanol/toluene/tetrahydrofuran/chloroform 64.3/21.5/13.7/0.5 v/v with 0.1% of triethylamine and a stoichiometric amount of formic acid. The molecular species were identified by LC/MS. The chromatographic behavior of DGDG on PGC was then compared to previous studies. The addition of a carbon double bond on the alkyl chain decreased the retention. This contribution was less important when the number of unsaturations increased in the alkyl chain. The consequence of this retention behavior with PGC was an elution order of molecular species which did not agree with the partition number as observed with C18 grafted stationary phases. PMID:15587281

  9. Changes in the chemical profile of cephalic salivary glands of Scaptotrigona postica (Hymenoptera, Meliponini) workers are phase related.

    PubMed

    Poiani, Silvana B; Morgan, E David; Drijfhout, Falko P; da Cruz-Landim, Carminda

    2015-09-01

    Most advanced eusocial bees recruit their nest mates to food resources. Recent studies in Meliponini species have revealed that the cephalic salivary (labial) glands (CSGs) are responsible for the production of scent trail pheromones. Studies on CSGs have shown that changes occur in worker glandular cell morphology from emergence from brood combs until forager phase, which may be correlated to changes in the composition of the CSG secretion. However, the composition of the CSG secretion and the chemical changes that occur in it according to the worker's life phase or tasks performed are unknown for many species, including Scaptotrigona postica. In this study, the chemical profile of CSG secretion in S. postica workers was studied. Glands were taken from specimens that were newly emerged (NE), working in the brood comb area (CA) and foraging (FO), and were analyzed by gas chromatography-mass spectrometry. The results showed that the glandular secretion consists of oxygenated compounds of middle volatility (acids, alcohols, aldehydes, ketones, esters and ether), and their quantity varies among the different life phases, increasing as the individual moves from intra- to extra-colonial activities. The NE phase contained the smallest variety and quantity of compounds. Because of the variability of compounds, the CA workers were separated into three subgroups according to the chemical constitution of their secretion. Forager workers showed the largest quantity and variety of chemical compounds. The major compounds in forager gland secretion were 7-hexadecen-1-yl acetate and 5-tetradecen-1-yl acetate. Statistical analysis indicates that the chemical composition of glandular secretion is phase related. PMID:26333928

  10. Transcriptional Profiling of Human Endocervical Tissues Reveals Distinct Gene Expression in the Follicular and Luteal Phases of the Menstrual Cycle.

    PubMed

    Yildiz-Arslan, Sevim; Coon, John S; Hope, Thomas J; Kim, J Julie

    2016-06-01

    The endocervix plays an important role in providing appropriate protective mechanisms of the upper female reproductive tract (FRT) while at the same time providing the appropriate milieu for sperm transport. Hormone fluctuations throughout the menstrual cycle contribute to changes in the mucosal environment that render the FRT vulnerable to infectious diseases. The objective of this study was to identify genes in human endocervix tissues that were differentially expressed in the follicular versus the luteal phases of the menstrual cycle using gene expression profiling. A microarray using the IIlumina platform was performed with eight endocervix tissues from follicular and four tissues from luteal phases of the menstrual cycle. Data analysis revealed significant differential expression of 110 genes between the two phases, with a P value <0.05 and a fold change cutoff of 1.5. Categorization of these genes, using Ingenuity Pathway Analysis, MetaCore from Thomson Reuters, and DAVID, revealed genes associated with extracellular matrix remodeling and cell-matrix interactions, amino acid metabolism, and lipid metabolism, as well as immune regulation in the follicular phase tissues. In luteal phase tissues, genes associated with chromatin remodeling, inflammation, angiogenesis, oxidative stress, and immune cell regulation were predominately expressed. Using samples from additional patients' tissues, select genes were confirmed by quantitative real-time PCR; immunohistochemical staining was also done to examine protein levels. This is the first microarray analysis comparing gene expression in endocervix tissues in cycling women. This study identified key genes and molecular pathways that were differentially regulated during the menstrual cycle. PMID:27170437

  11. Imaging of the Deep Structure by Long Term Broad Band OBSs \\ \\ -- Trans-PHS Profile and NW Pacific WP-2 site --

    NASA Astrophysics Data System (ADS)

    Shiobara, H.; Sugioka, H.; Yoneshima, S.; Mochizuki, K.; Mochizuki, M.; Kodaira, S.; Hino, R.; Shinohara, M.; Kanazawa, T.; Fukao, Y.

    2001-12-01

    As an important part of the Ocean Hemisphere network Project, long term ocean bottom seismic observations have been started and some of their data have been retrieved already. One of them, the trans-Philippine Sea profile observation (15 Long Term OBSs, Nov. 1999 -- July 2000) was preliminarily reported at the last AGU fall meeting (S51B-02, 2000). Other long term broad band observations had been performed twice at the northwestern Pacific (Broad Band OBS, NWPAC1: Aug. 1999 -- May 2000, NWPAC2: May 2000 -- Nov. 2000), where the borehole seismic observatory (WP-2) has been activated in Nov. 2000. The LTOBS and the BBOBS contain a semi broad band sensor (WB2023LP, PMD) which has the pass band from 30s to 50Hz and a broad band sensor (CMG-1T, Guralp) which has the pass band from 360s to 80Hz, respectively. The seismic data is recorded continuously with a sampling frequency of 128Hz by a 20bit ADC on four 2.5 inch 6.5 GB HDDs. The direction of horizontal components are determined from the data of the direct water wave during the airgun shooting or P-SV converted waves at the sediment-basement interface. Running acceleration power spectra (0dB=1m2/s4/Hz) of these data during the whole observation period indicate followings; 1) sufficiently low noise band exists in the frequency range of 10--100mHz and the lowest level is close to the NLNM, but the horizontal noise level varies about 20--30dB with a dominant 12 hours interval, 2) high level micro seismic noise in the range of 0.1--1Hz is always near the NHNM and varies about 30--40dB. In this presentation, preliminary results of the deep structure imaging from these vast data by a receiver function analysis is shown. To perform this analysis with the OBS data, removal of water column reverberations and reduction of high level micro seismic noise should be necessary. The former process has a difficulty in the estimation of the water-sea bottom reflection coefficient and requires the data of higher sampling frequency, more

  12. Effect of resistivity profile on current decay time of initial phase of current quench in neon-gas-puff inducing disruptions of JT-60U

    NASA Astrophysics Data System (ADS)

    Kawakami, S.; Shibata, Y.; Watanabe, K. Y.; Ohno, N.; Isayama, A.; Takizuka, T.; Kawano, Y.; Okamoto, M.

    2013-11-01

    According to an early work [Y. Shibata et al., Nucl. Fusion 50, 025015 (2010)] on the behavior of the plasma current decay in the JT-60U disruptive discharges caused by the radiative collapse with a massive neon-gas-puff, the increase of the internal inductance mainly determined the current decay time of plasma current during the initial phase of current quench. To investigate what determines the increase of the internal inductance, we focus attention on the relationship between the electron temperature (or the resistivity) profile and the time evolution of the current density profile and carry out numerical calculations. As a result, we find the reason of the increase of the internal inductance: The current density profile at the start of the current quench is broader than an expected current density profile in the steady state, which is determined by the temperature (or resistivity) profile. The current density profile evolves into peaked one and the internal inductance is increasing.

  13. Effect of resistivity profile on current decay time of initial phase of current quench in neon-gas-puff inducing disruptions of JT-60U

    SciTech Connect

    Kawakami, S.; Ohno, N.; Shibata, Y.; Isayama, A.; Kawano, Y.; Watanabe, K. Y.; Takizuka, T.; Okamoto, M.

    2013-11-15

    According to an early work [Y. Shibata et al., Nucl. Fusion 50, 025015 (2010)] on the behavior of the plasma current decay in the JT-60U disruptive discharges caused by the radiative collapse with a massive neon-gas-puff, the increase of the internal inductance mainly determined the current decay time of plasma current during the initial phase of current quench. To investigate what determines the increase of the internal inductance, we focus attention on the relationship between the electron temperature (or the resistivity) profile and the time evolution of the current density profile and carry out numerical calculations. As a result, we find the reason of the increase of the internal inductance: The current density profile at the start of the current quench is broader than an expected current density profile in the steady state, which is determined by the temperature (or resistivity) profile. The current density profile evolves into peaked one and the internal inductance is increasing.

  14. Three-dimensional profiling with binary fringes using phase-shifting interferometry algorithms

    SciTech Connect

    Ayubi, Gaston A.; Di Martino, J. Matias; Alonso, Julia R.; Fernandez, Ariel; Perciante, Cesar D.; Ferrari, Jose A.

    2011-01-10

    Three-dimensional shape measurements by sinusoidal fringe projection using phase-shifting interferometry algorithms are distorted by the nonlinear response in intensity of commercial video projectors and digital cameras. To solve the problem, we present a method that consists in projecting and acquiring a temporal sequence of strictly binary patterns, whose (adequately weighted) average leads to a sinusoidal fringe pattern with the required number of bits. Since binary patterns consist of ''ones'' and ''zeros'' - and no half-tones are involved - the nonlinear response of the projector and the camera will not play a role, and a nearly unit contrast gray-level sinusoidal fringe pattern is obtained. Validation experiments are presented.

  15. Depth profiling of photothermal compound concentrations using phase sensitive optical coherence tomography

    PubMed Central

    Guan, Guangying; Reif, Roberto; Huang, Zhihong; Wang, Ruikang K.

    2011-01-01

    A model that describes the concentration of photothermal (light-to-heat converters) compounds as a function of depth in a turbid medium is developed. The system consists of a pump laser (808 nm modulated at 400 Hz), which heats a photothermal compound, and a phase sensitive spectral domain optical coherence tomography system, which detects the changes in the optical path length of the sample induced by the temperature increase. The model is theoretically derived and the coefficients are empirically determined using solid homogeneous gel phantoms. The model is validated by reconstructing the concentration of a photothermal compound in thick single and double layer solid phantoms. PMID:22191920

  16. Modeling the zonal disintegration of rocks near deep level tunnels by gradient internal variable continuous phase transition theory

    NASA Astrophysics Data System (ADS)

    Haoxiang, Chen; Qi, Chengzhi; Peng, Liu; Kairui, Li; Aifantis, Elias C.

    2015-12-01

    The occurrence of alternating damage zones surrounding underground openings (commonly known as zonal disintegration) is treated as a "far from thermodynamic equilibrium" dynamical process or a nonlinear continuous phase transition phenomenon. The approach of internal variable gradient theory with diffusive transport, which may be viewed as a subclass of Landau's phase transition theory, is adopted. The order parameter is identified with an irreversible strain quantity, the gradient of which enters into the expression for the free energy of the rock system. The gradient term stabilizes the material behavior in the post-softening regime, where zonal disintegration occurs. The results of a simplified linearized analysis are confirmed by the numerical solution of the nonlinear problem.

  17. Clinical profile of chikungunya sequelae, association with obesity and rest during acute phase.

    PubMed

    Padmakumar, B; Jayan, Jacob B; Menon, Rejeesh; Kottarathara, Arun Jose

    2010-01-01

    The scarcity of literature regarding chikungunya infection sequelae makes it an unexplored area of medicine. We analyzed 1,111 patients with confirmed chikungunya sequelae and found a female predominance in those with sequelae which increased with age up to 40-50 years old, then decreased with further increase in age. In males age > 60 years old was the predominant age group affected. The symptoms were mainly symmetrical polyarthralgia of the proximal and distal interphalangeal joints. Dermatological manifestations were mainly hyper pigmented patches, generalized pruritus, and a maculopapular rash. Insomnia, fatigability and headache may indicate neurological involvement. Obesity gave an odds ratio of 2.07 for risk of arthritis. There was no significant benefit from rest during the acute phase (p < 0.001) of chikungunya in preventing chronicity of sequelae. Obesity as an independent risk factor for chronicity of chikungunya infection sequelae is a new finding. PMID:20578486

  18. High latitude proton precipitation and light-ion density profiles during the magnetic storm initial phase

    NASA Technical Reports Server (NTRS)

    Burch, J. L.

    1973-01-01

    Measurements of precipitating protons and light ion densities by experiments on OGO-4 indicate that widespread proton precipitation occurs in predawn hours during the magnetic storm initial phase from the latitude of the high-latitude ion trough, or plasmapause , up to Lambda 75 deg. A softening of the proton spectrum is apparent as the plasmapause is approached. The separation of the low-latitude precipitation boundaries for 7.3 kev and 23.8 kev protons is approximately 1 deg, compared with a 3.6 deg separation which has been computed using the formulas of Gendrin and Eather and Carovillano. Consideration of probable proton drift morphology leads to the conclusion that protons ase injected in predawn hours, with widespread precipitation occurring in the region outside the plasmapause. Protons less energetic than approximately 7 kev drift eastward, while the more energetic protons drift westward, producing the observed dawn-dusk asymmetry for the lower-energy protons.

  19. Mass transfer model liquid phase catalytic exchange column simulation applicable to any column composition profile

    SciTech Connect

    Busigin, A.

    2015-03-15

    Liquid Phase Catalytic Exchange (LPCE) is a key technology used in water detritiation systems. Rigorous simulation of LPCE is complicated when a column may have both hydrogen and deuterium present in significant concentrations in different sections of the column. This paper presents a general mass transfer model for a homogenous packed bed LPCE column as a set of differential equations describing composition change, and equilibrium equations to define the mass transfer driving force within the column. The model is used to show the effect of deuterium buildup in the bottom of an LPCE column from non-negligible D atom fraction in the bottom feed gas to the column. These types of calculations are important in the design of CECE (Combined Electrolysis and Catalytic Exchange) water detritiation systems.

  20. Authigenic phase formation and microbial activity control Zr, Hf, and rare earth element distributions in deep-sea brine sediments

    NASA Astrophysics Data System (ADS)

    Censi, P.; Saiano, F.; Zuddas, P.; Nicosia, A.; Mazzola, S.; Raso, M.

    2014-02-01

    Sediments collected from hypersaline and anoxic deep-sea basins in the eastern Mediterranean (Thetis, Kryos, Medee, and Tyro) were characterised in terms of their mineralogical composition, the distributions of rare earth elements (REE), Zr, and Hf and their content of microbial DNA. We identified two major mineralogical fractions: one fraction of detritic origin was composed of quartz, gypsum, and low-Mg calcite bioclasts (with 0 < Mg < 0.07%) and another fraction of authigenic origin constituted of halite, dolomite, high-Mg calcite (with a Mg content of up to 22%) and rare bischofite and showed a textural evidence of microbial assemblages. We found that in the Medee and Tyro sediments, the shale-normalised REE pattern of these sediments is strongly enriched in middle REE (MREE), whereas in the Thetis and Tyro basins, a positive Gd anomaly in the residue was obtained after the removal of the water-soluble fraction. In all investigated basins, Y / Ho ratio clustered around chondritic values, whereas Zr / Hf ratio ranged from slightly subchondritic to superchondritic values. Subchondritic Y / Ho and Zr / Hf values were mainly found in the high-Mg carbonate having a microbial origin. The observed preferential removal of Zr with respect to Hf without significant partitioning of Y with respect to Ho indicates that the Zr / Hf ratio and Y-Ho fractionations are influenced by the microbial activity in the sediments. We propose that the concurrent Y-Ho and Zr-Hf fractionations are a suitable tracer of microbial activity in marine sediments.

  1. Neurological effects of deep diving.

    PubMed

    Grønning, Marit; Aarli, Johan A

    2011-05-15

    Deep diving is defined as diving to depths more than 50 m of seawater (msw), and is mainly used for occupational and military purposes. A deep dive is characterized by the compression phase, the bottom time and the decompression phase. Neurological and neurophysiologic effects are demonstrated in divers during the compression phase and the bottom time. Immediate and transient neurological effects after deep dives have been shown in some divers. However, the results from the epidemiological studies regarding long term neurological effects from deep diving are conflicting and still not conclusive. Prospective clinical studies with sufficient power and sensitivity are needed to solve this very important issue. PMID:21377169

  2. Toxicity and composition profiles of solid phase extracts of oil sands process-affected water.

    PubMed

    Yue, Siqing; Ramsay, Bruce A; Wang, Jiaxi; Ramsay, Juliana

    2015-12-15

    After fractionation using sequential solid phase extraction, the presence of toxic components in oil sands process-affected water (OSPW) was detected by the Microtox® acute toxicity assay using effect-directed analysis. The composition of each fraction was determined by high-resolution electrospray ionization-Orbitrap mass spectrometry. Partial least-squares discriminant analysis (PLS-DA) was used to determine which chemical constituents in all seven fractions co-varied most strongly with toxicity. Although O2 compounds with double bond equivalence (DBE) between 3 and 9 positively correlated with toxicity, C15-C18 O2-NAs with DBE=4 (tricyclic structure), as well as C14-C17 O2-NAs with DBE=3 (bicyclic structure), were found to be most likely associated with OSPW toxicity, consistent with published toxicity studies of surrogate NAs. O4, many O3 (i.e. possibly hydroxylated O2 c-NAs) and a few O2 compounds were found to negatively correlate with toxicity. The results demonstrate the utility of the fractionation and the PLS-DA approach for evaluating composition-response relationships in a complex mixture and also contribute to a better understanding of the toxic compounds in OSPW. These findings will help to focus study on the most environmentally significant components in OSPW. PMID:26318810

  3. The semiannual variation of geomagnetic activity: Phases and profiles for 130 years of aa data

    NASA Astrophysics Data System (ADS)

    Cliver, E. W.; Kamide, Y.; Ling, A. G.

    2001-05-01

    We determined the phases of the maxima (spring, fall) and minima (summer, winter) in the curve of smoothed daily averages of the aa geomagnetic index, available from 1868-1998. The dates we obtained are consistent with the equinoctial hypothesis which has aberration-adjusted theoretical maxima on 25 March (experimentally determined to be 27 March) and 27 September (27 September) and minima on 26 June (26 June) and 26 December (27 December). We also show that the overall shape of the modulation curve throughout the year (broad minima, narrow peaks) bears greater fidelity (r = -0.96) to the solar declination D (the controlling angle for the seasonal variation under the equinoctial hypothesis) than to the solar B angle (r = 0.83; axial hypothesis) or the solar P angle (r = 0.80; Russell-McPherron effect). Lastly, a three-parameter fit of the smoothed annual variation of the aa data with a function consisting of the sum of the smoothed yearly curves for the D, B, and P angles yielded an amplitude of 0.58 for the D component vs. 0.20 for B and 0.16 for P. Generally similar results for each of these analyses (timing, shape, relative contributions) were obtained for shorter intervals of data for the ap and am indices. We conclude that the semiannual modulation of average values of mid-latitude range indices such as aa and ap is primarily controlled by the equinoctial hypothesis.

  4. High-latitude proton precipitation and light ion density profiles during the magnetic storm initial phase.

    NASA Technical Reports Server (NTRS)

    Burch, J. L.

    1973-01-01

    Measurements of precipitating protons and light ion densities by experiments on Ogo 4 indicate that widespread proton precipitation occurs in predawn hours during the magnetic storm initial phase from the latitude of the high-latitude ion trough, or plasmapause, up to latitudes greater than 75 deg. A softening of the proton spectrum is apparent as the plasmapause is approached. The separation of the low-latitude precipitation boundaries for 7.3-keV and 23.8-keV protons is less than about 1 deg, compared with a 3.6-deg separation that has been computed by using the formulas of Gendrin and Eather and Carovillano. Consideration of probable proton drift morphology leads to the conclusion that protons are injected in predawn hours, widespread precipitation occurring in the region outside the plasmapause. Protons less energetic than 7 keV drift eastward, whereas the more energetic protons drift westward, producing the observed dawn-dusk asymmetry for the lower-energy protons.

  5. A UNIVERSAL POWER-LAW PROFILE OF PSEUDO-PHASE-SPACE DENSITY-LIKE QUANTITIES IN ELLIPTICAL GALAXIES

    SciTech Connect

    Chae, Kyu-Hyun

    2014-06-10

    We study profiles of mass density, velocity dispersion (VD), and a combination of both using ∼2000 nearly spherical and rotation-free Sloan Digital Sky Survey galaxies. For observational stellar mass density ρ{sub *}(r), we consider a range of dark matter (DM) distribution ρ{sub DM}(r) and VD anisotropy β(r) to investigate radial stellar VD σ{sub *r}(r) using the spherical Jeans equation. While mass and VD profiles vary appreciably depending on DM distribution and anisotropy, the pseudo-phase-space density-like combination ρ(r)/σ{sub ⋆r}{sup 3}(r) with total density ρ(r) = ρ{sub *}(r) + ρ{sub DM}(r) is nearly universal. In the optical region, the negative logarithmic slope has a mean value of (χ) ≈ 1.86-1.90 with a galaxy-to-galaxy rms scatter of ≈0.04-0.06, which is a few times smaller than that of ρ(r) profiles. The scatter of χ can be increased by invoking wildly varying anisotropies that are, however, less likely because they would produce too large a scatter of line of sight VD profiles. As an independent check of this universality, we analyze stellar orbit-based dynamical models of 15 early-type galaxies (ETGs) of the Coma cluster provided by J. Thomas. Coma ETGs, with σ{sub *r}(r) replaced by the rms velocity of stars v {sub *rms}(r) including net rotation, exhibit a similar universality with a slope of χ = 1.93 ± 0.06. Remarkably, the inferred values of χ for ETGs match well the slope ≈1.9 predicted by N-body simulations of DM halos. We argue that the inferred universal nature of ρ(r)/σ{sub ⋆r}{sup 3}(r) cannot be fully explained by equilibrium alone, implying that some astrophysical factors conspire and/or it reflects a fundamental principle in collisionless formation processes.

  6. Experimental multi-phase H2O-CO2 brine interactions at elevated temperature and pressure: Implications for CO2 sequestration in deep-saline aquifers

    USGS Publications Warehouse

    Rosenbauer, R.; Koksalan, T.

    2004-01-01

    The burning of fossil fuel and other anthropogenic activities have caused a continuous and dramatic 30% increase of atmospheric CO2 over the past 150 yr. CO2 sequestration is increasingly being viewed as a tool for managing these anthropogenic CO2 emissions to the atmosphere. CO2-saturated brine-rock experiments were carried out to evaluate the effects of multiphase H2O-CO2 fluids on mineral equilibria and the potential for CO2 sequestration in mineral phases within deep-saline aquifers. Experimental results were generally consistent with theoretical thermodynamic calculations. The solubility of CO2 was enhanced in brines in the presence of both limestone and sandstone relative to brines alone. Reactions between CO2 saturated brines and arkosic sandstones were characterized by desiccation of the brine and changes in the chemical composition of the brine suggesting fixation of CO2 in mineral phases. These reactions were occurring on a measurable but kinetically slow time scale at 120??C.

  7. Experimental multi-phase CO2-brine-rock interactions at elevated temperature and pressure: Implications for CO2 sequestration in deep-saline aquifers

    USGS Publications Warehouse

    Rosenbauer, R.J.; Koksalan, T.

    2004-01-01

    Long-term CO2 saturated brine-rock experiments were conducted to evaluate the effects of multiphase H2O-CO2 fluids on mineral equilibria and the potential for CO2 sequestration mineral phases within deep-saline aquifers. Experimental results were consistent with theoretical thermodynamic calculations when CO2-saturated brines were reacted with limestone rocks. The CO2-saturated brine-limestone reactions were characterized by compositional and mineralogical-changes in the aquifer fluid and formation rocks that were dependent on initial brine composition as were the changes in formation porosity, especially dissolved sulfate. The solubility of CO2 was enhanced in brines in the presence of both limestone and sandstone rocks relative to brines alone. Reactions between CO2 saturated brines and arkosic sandstones were characterized by desiccation of the brine and changes in the chemical composition of the brine suggesting fixation of CO2 in mineral phases. These reactions occured on a measurable but kinetically slow time scale at 120??C.

  8. Phase-Amplitude Coupling Is Elevated in Deep Sleep and in the Onset Zone of Focal Epileptic Seizures

    PubMed Central

    Amiri, Mina; Frauscher, Birgit; Gotman, Jean

    2016-01-01

    The interactions between different EEG frequency bands have been widely investigated in normal and pathologic brain activity. Phase-amplitude coupling (PAC) is one of the important forms of this interaction where the amplitude of higher frequency oscillations is modulated by the phase of lower frequency activity. Here, we studied the dynamic variations of PAC of high (gamma and ripple) and low (delta, theta, alpha, and beta) frequency bands in patients with focal epilepsy in different sleep stages during the interictal period, in an attempt to see if coupling is different in more or less epileptogenic regions. Sharp activities were excluded to avoid their effect on the PAC. The results revealed that the coupling intensity was generally the highest in stage N3 of sleep and the lowest in rapid eye movement sleep. We also compared the coupling strength in different regions [seizure onset zone (SOZ), exclusively irritative zone, and normal zone]. PAC between high and low frequency rhythms was found to be significantly stronger in the SOZ compared to normal regions. Also, the coupling was generally more elevated in spiking channels outside the SOZ than in normal regions. We also examined how the power in the delta band correlates to the PAC, and found a mild but statistically significant correlation between slower background activity in epileptic channels and the elevated coupling in these channels. The results suggest that an elevated PAC may reflect some fundamental abnormality, even after exclusion of sharp activities and even in the interictal period. PAC may therefore contribute to understanding the underlying dynamics of epileptogenic brain regions. PMID:27536227

  9. Phase-Amplitude Coupling Is Elevated in Deep Sleep and in the Onset Zone of Focal Epileptic Seizures.

    PubMed

    Amiri, Mina; Frauscher, Birgit; Gotman, Jean

    2016-01-01

    The interactions between different EEG frequency bands have been widely investigated in normal and pathologic brain activity. Phase-amplitude coupling (PAC) is one of the important forms of this interaction where the amplitude of higher frequency oscillations is modulated by the phase of lower frequency activity. Here, we studied the dynamic variations of PAC of high (gamma and ripple) and low (delta, theta, alpha, and beta) frequency bands in patients with focal epilepsy in different sleep stages during the interictal period, in an attempt to see if coupling is different in more or less epileptogenic regions. Sharp activities were excluded to avoid their effect on the PAC. The results revealed that the coupling intensity was generally the highest in stage N3 of sleep and the lowest in rapid eye movement sleep. We also compared the coupling strength in different regions [seizure onset zone (SOZ), exclusively irritative zone, and normal zone]. PAC between high and low frequency rhythms was found to be significantly stronger in the SOZ compared to normal regions. Also, the coupling was generally more elevated in spiking channels outside the SOZ than in normal regions. We also examined how the power in the delta band correlates to the PAC, and found a mild but statistically significant correlation between slower background activity in epileptic channels and the elevated coupling in these channels. The results suggest that an elevated PAC may reflect some fundamental abnormality, even after exclusion of sharp activities and even in the interictal period. PAC may therefore contribute to understanding the underlying dynamics of epileptogenic brain regions. PMID:27536227

  10. Cohort profile: LifeLines DEEP, a prospective, general population cohort study in the northern Netherlands: study design and baseline characteristics

    PubMed Central

    Tigchelaar, Ettje F; Zhernakova, Alexandra; Dekens, Jackie A M; Hermes, Gerben; Baranska, Agnieszka; Mujagic, Zlatan; Swertz, Morris A; Muñoz, Angélica M; Deelen, Patrick; Cénit, Maria C; Franke, Lude; Scholtens, Salome; Stolk, Ronald P; Wijmenga, Cisca; Feskens, Edith J M

    2015-01-01

    Purpose There is a critical need for population-based prospective cohort studies because they follow individuals before the onset of disease, allowing for studies that can identify biomarkers and disease-modifying effects, and thereby contributing to systems epidemiology. Participants This paper describes the design and baseline characteristics of an intensively examined subpopulation of the LifeLines cohort in the Netherlands. In this unique subcohort, LifeLines DEEP, we included 1539 participants aged 18 years and older. Findings to date We collected additional blood (n=1387), exhaled air (n=1425) and faecal samples (n=1248), and elicited responses to gastrointestinal health questionnaires (n=1176) for analysis of the genome, epigenome, transcriptome, microbiome, metabolome and other biological levels. Here, we provide an overview of the different data layers in LifeLines DEEP and present baseline characteristics of the study population including food intake and quality of life. We also describe how the LifeLines DEEP cohort allows for the detailed investigation of genetic, genomic and metabolic variation for a wide range of phenotypic outcomes. Finally, we examine the determinants of gastrointestinal health, an area of particular interest to us that can be addressed by LifeLines DEEP. Future plans We have established a cohort of which multiple data levels allow for the integrative analysis of populations for translation of this information into biomarkers for disease, and which will offer new insights into disease mechanisms and prevention. PMID:26319774

  11. Organohalogen compounds in deep-sea fishes from the western North Pacific, off-Tohoku, Japan: Contamination status and bioaccumulation profiles.

    PubMed

    Takahashi, Shin; Oshihoi, Tomoko; Ramu, Karri; Isobe, Tomohiko; Ohmori, Koji; Kubodera, Tsunemi; Tanabe, Shinsuke

    2010-02-01

    Twelve species of deep-sea fishes collected in 2005 from the western North Pacific, off-Tohoku, Japan were analyzed for organohalogen compounds. Among the compounds analyzed, concentrations of DDTs and PCBs (up to 23,000 and 12,400 ng/g lipid wt, respectively) were the highest. The present study is the foremost to report the occurrence of brominated flame retardants such as PBDEs and HBCDs in deep-sea organisms from the North Pacific region. Significant positive correlations found between delta(15)N ( per thousand) and PCBs, DDTs and PBDEs suggest the high biomagnification potential of these contaminants in food web. The large variation in delta(13)C (per thousand) values observed between the species indicate multiple sources of carbon in the food web and specific accumulation of hydrophobic organohalogen compounds in benthic dwelling carnivore species like snubnosed eel. The results obtained in this study highlight the usefulness of deep-sea fishes as sentinel species to monitor the deep-sea environment. PMID:19837437

  12. 30 CFR 203.43 - To which production do I apply the RSV earned from qualified deep wells or qualified phase 1...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... production occurring on and after the later of: (i) May 3, 2004, for an RSV earned by a qualified deep well... interval the top of which is 16,600 feet TVD SS, which becomes a qualified deep well when production begins... production in August 2009, it would not be a qualified deep well because it started production...

  13. Stable Deep Doping of Vapor-Phase Polymerized Poly(3,4-ethylenedioxythiophene)/Ionic Liquid Supercapacitors.

    PubMed

    Karlsson, Christoffer; Nicholas, James; Evans, Drew; Forsyth, Maria; Strømme, Maria; Sjödin, Martin; Howlett, Patrick C; Pozo-Gonzalo, Cristina

    2016-08-23

    Liquid-solution polymerization and vapor-phase polymerization (VPP) have been used to manufacture a series of chloride- and tosylate-doped poly(3,4-ethylenedioxythiophene) (PEDOT) carbon paper electrodes. The electrochemistry, specific capacitance, and specific charge were determined for single electrodes in 1-ethyl-3-methylimidazolium dicyanamide (emim dca) ionic liquid electrolyte. VPP-PEDOT exhibits outstanding properties with a specific capacitance higher than 300 F g(-1) , the highest value reported for a PEDOT-based conducting polymer, and doping levels as high as 0.7 charges per monomer were achieved. Furthermore, symmetric PEDOT supercapacitor cells with the emim dca electrolyte exhibited a high specific capacitance (76.4 F g(-1) ) and high specific energy (19.8 Wh kg(-1) ). A Ragone plot shows that the VPP-PEDOT cells combine the high specific power of conventional ("pure") capacitors with the high specific energy of batteries, a highly sought-after target for energy storage. PMID:27325487

  14. Analysis of genes that influence sheep follicular development by different nutrition levels during the luteal phase using expression profiling.

    PubMed

    Luo, F; Jia, R; Ying, S; Wang, Z; Wang, F

    2016-06-01

    Nutrition is an important factor that regulates reproductive performance of sheep and affects follicle development. However, the correlation between nutrition and follicle development is poorly understood at the molecular level. To study its possible molecular mechanisms, we performed expression profiling of granulosa cells isolated from sheep that were fed different levels of nutrition levels during the luteal phase. To do this, ewes received a maintenance diet (M), and their estrus was synchronized by intravaginal progestogen sponges for 12 days. Ewes were randomly divided into the short-term dietary-restricted group (R; 0.5 × M) and the nutrient-supplemented group (S; 1.5 × M). RNA samples were extracted from granulosa cells. Transcriptome libraries from each group were constructed by Illumina sequencing. Among 18 468 detected genes, 170 genes were significantly differentially expressed, of which 140 genes were upregulated and 30 genes were downregulated in group S relative to group R. These genes could be candidates regulating follicular development in sheep. Gene Ontology, KEGG and clustering analyses were performed. Genes related to oocyte meiosis, such as ADCY7, were upregulated. We identified two important groups of related genes that were upregulated with improved nutrition: one group comprising the genes PTGS2, UCP2 and steroidogenic acute regulatory protein and the other group comprising interleukin-1A and interleukin-1B. The genes within each group showed similar expression patterns. Additionally, all five genes are involved in the reproduction process. Quantitative real-time PCR was performed to validate the results of expression profiling. These data in our study are an abundant genomic resource to expand the understanding of the molecular and cellular events underlying follicle development. PMID:26970339

  15. Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry.

    PubMed

    Romoli, Riccardo; Papaleo, Maria Cristiana; de Pascale, Donatella; Tutino, Maria Luisa; Michaud, Luigi; LoGiudice, Angelina; Fani, Renato; Bartolucci, Gianluca

    2011-10-01

    Bacteria belonging to the Burkholderia cepacia complex (Bcc) are significant pathogens in Cystic Fibrosis (CF) patients and are resistant to a plethora of antibiotics. In this context, microorganisms from Antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. This is particularly true for bacteria isolated from Antarctic sponges. The aim of this work was to characterize a set of Antarctic bacteria for their ability to produce new natural drugs that could be exploited in the control of infections in CF patients by Bcc bacteria. Hence, 11 bacterial strains allocated to different genera (e.g., Pseudoalteromonas, Arthrobacter and Psychrobacter) were tested for their ability to inhibit the growth of 21 Bcc strains and some other human pathogens. All these bacteria completely inhibited the growth of most, if not all, Bcc strains, suggesting a highly specific activity toward Bcc strains. Experimental evidences showed that the antimicrobial compounds are small volatile organic compounds, and are constitutively produced via an unknown pathway. The microbial volatile profile was obtained by SPME-GC-MS within the m/z interval of 40-450. Solid phase micro extraction technique affords the possibility to extract the volatile compounds in head space with a minimal sample perturbation. Principal component analysis and successive cluster discriminant analysis was applied to evaluate the relationships among the volatile organic compounds with the aim of classifying the microorganisms by their volatile profile. These data highlight the potentiality of Antarctic bacteria as novel sources of antibacterial substances to face Bcc infections in CF patients. PMID:22012672

  16. Transverse-to-longitudinal phase space exchange: a versatile tool for shaping the current and energy profiles of relativistic electron bunches

    SciTech Connect

    Piot, P.; Sun, Y.-E.; Johnson, A.S.; Lumpkin, A.H.; Maxwell, T.; Ruan, J.; Thurman-Keup, R.; /Fermilab

    2010-08-01

    Over the recent years, the emergence of accelerator beamlines capable of exchanging the phase space coordinates between two degrees of freedom have opened the path toward the precise control of phase space distribution and in particular to the production of relativistic electron beams with shaped current profiles. After briefly reviewing the technique, we present its application to produce a train of sub-picosecond microbunches and report on its experimental implementation at the Fermilab's A0 photoinjector facility.

  17. Probing the probe: AFM tip-profiling via nanotemplates to determine Hamaker constants from phase-distance curves.

    PubMed

    Rodriguez, Raul D; Lacaze, Emmanuelle; Jupille, Jacques

    2012-10-01

    A method to determine the van der Waals forces from phase-distance curves recorded by atomic force microscopy (AFM) in tapping mode is presented. The relationship between the phase shift and the tip-sample distance is expressed as a function of the product of the Hamaker constant by tip radius. Silica-covered silicon tips are used to probe silica-covered silicon substrate in dry conditions to avoid capillary effects. Tips being assumed spherical, radii are determined in situ by averaging profiles recorded in different directions on hematite nanocrystals acting as nanotemplates, thus accounting for tip anisotropy. Through a series of reproducible measurements performed with tips of various radii (including the in-situ characterization of a damaged tip), a value of (6.3±0.4)×10(-20) J is found for the Hamaker constant of interacting silica surfaces in air, in good agreement with tabulated data. The results demonstrate that the onset of the tip-surface interaction is dominated by the van der Waals forces and that the total force can be modeled in the framework of the harmonic approximation. Based on the tip radius and the Hamaker constant associated to the tip-substrate system, the model is quite flexible. Once the Hamaker constant is known, a direct estimate of the tip size can be achieved whereas when the tip size is known, a quantitative evaluation of the van der Waals force becomes possible on different substrates with a spatial resolution at the nanoscale. PMID:22922181

  18. The spatial extent of the Deep Western Boundary Current into the Bounty Trough: new evidence from parasound sub-bottom profiling

    NASA Astrophysics Data System (ADS)

    Horn, Michael; Uenzelmann-Neben, Gabriele

    2016-04-01

    Deep currents such as the Pacific Deep Western Boundary Current (DWBC) are strengthened periodically in Milankovitch cycles. We studied periodic fluctuations in seismic reflection pattern and reflection amplitude in order to detect cycles in the sedimentary layers of Bounty Trough and bounty fan, east of New Zealand. There, the occurrence of the obliquity frequency is caused only by the DWBC. Therefore, it provides direct evidence for the spatial extent of the DWBC. We can confirm the extent of the DWBC west of the outer sill, previously only inferred via erosional features at the outer sill. Further, our data allow an estimation of the extent of the DWBC into the Bounty Trough, limiting the DWBC presence to east of 178.15°E. Using the presented method a larger dataset will allow a chronological and areal mapping of sedimentation processes and hence provide information on glacial/interglacial cycles.

  19. The spatial extent of the Deep Western Boundary Current into the Bounty Trough: new evidence from parasound sub-bottom profiling

    NASA Astrophysics Data System (ADS)

    Horn, Michael; Uenzelmann-Neben, Gabriele

    2016-06-01

    Deep currents such as the Pacific Deep Western Boundary Current (DWBC) are strengthened periodically in Milankovitch cycles. We studied periodic fluctuations in seismic reflection pattern and reflection amplitude in order to detect cycles in the sedimentary layers of Bounty Trough and bounty fan, east of New Zealand. There, the occurrence of the obliquity frequency is caused only by the DWBC. Therefore, it provides direct evidence for the spatial extent of the DWBC. We can confirm the extent of the DWBC west of the outer sill, previously only inferred via erosional features at the outer sill. Further, our data allow an estimation of the extent of the DWBC into the Bounty Trough, limiting the DWBC presence to east of 178.15°E. Using the presented method a larger dataset will allow a chronological and areal mapping of sedimentation processes and hence provide information on glacial/interglacial cycles.

  20. Bacterial processes in the intermediate and deep layers of the Ionian Sea in winter 1999: Vertical profiles and their relationship to the different water masses

    NASA Astrophysics Data System (ADS)

    Zaccone, R.; Monticelli, L. S.; Seritti, A.; Santinelli, C.; Azzaro, M.; Boldrin, A.; La Ferla, R.; Ribera D'Alcalã, M.

    2003-09-01

    Dissolved and particulate organic carbon, bacterial biomass, microbial enzymatic activities (EEA: leucine aminopeptidase, β-glucosidase, and alkaline phosphatase), bacterial production, respiration rates, and bacterial growth efficiency were determined in 10 stations of the Ionian Sea (winter 1998-1999) with the aim of characterizing the recycling of biogenic carbon and phosphorus in the different water masses, previously identified on the basis of their hydrographical properties. All microbial activities decreased markedly with depth, with a sharp increase in the benthic boundary layer, where potential remineralization rates of phosphorus up to 1.03 μg P·dm-3d-1 and bacterial carbon production of 0.078 μg C·dm-3 d-1 were recorded. Those rates were close to the surface ones; the bacterial growth efficiency was also around 20%, similar to the surface value, sustaining the microbial food chain at the bottom. The daily hydrolysis of the organic carbon pool estimated by EEA varied from 0.67% (Ionian Surface Water) to 0.02% (Deep Water). Alkaline phosphatase activity was generally low in the intermediate and deep layers, in relation to the higher inorganic P content. The last facts support the hypothesis that deep waters of Ionian Sea, and in general of the entire Mediterranean basin, because of their young age, carry a larger amount of labile dissolved organic carbon, which reduces the need for a high recycling activity by bacterial community. As a matter of fact, a relatively higher activity per cell in carbon production rates was found in the deep layer where a large volume of the very recently formed Cretan Sea Outflow Water was present.

  1. Phase I study of tivantinib in Japanese patients with advanced hepatocellular carcinoma: Distinctive pharmacokinetic profiles from other solid tumors.

    PubMed

    Okusaka, Takuji; Aramaki, Takeshi; Inaba, Yoshitaka; Nakamura, Shinichiro; Morimoto, Manabu; Moriguchi, Michihisa; Sato, Takashi; Ikawa, Yuta; Ikeda, Masafumi; Furuse, Junji

    2015-05-01

    A c-Met inhibitor tivantinib is a candidate anticancer agent for patients with hepatocellular carcinoma (HCC), and CYP2C19 is the key metabolic enzyme for tivantinib. Previous Japanese phase I studies in patients with solid tumors (except HCC) recommend 360 mg twice daily (BID) and 240 mg BID for CYP2C19 extensive metabolizers (EM) and poor metabolizers (PM), respectively. In this study, Japanese patients with HCC in whom sorafenib treatment has failed were enrolled to evaluate the safety, tolerability and pharmacokinetics of oral tivantinib as a single agent. The dose was escalated separately in EM and PM, from 120 mg BID to 240 mg BID, in both capsule and tablet formulations. A total of 28 patients (EM: 21, PM: 7) received tivantinib. At a dose of 120 mg BID, dose-limiting toxicities (DLT) did not develop in 12 EM (capsule: 6, tablet: 6) and 7 PM (capsule: 4, tablet: 3) during the DLT-observation period (for 29 days after first dosing). At this dose, the pharmacokinetic profiles of tivantinib (AUC0-12 and Cmax ) did not remarkably differ between EM and PM. When treated with 240 mg BID, 5 of 9 EM (capsule: 4 of 6, tablet: 1 of 3) developed neutropenia-related DLT accompanying plasma tivantinib concentration higher than expected from the previous studies. Consequently, PM did not receive 240 mg BID. In conclusion, 120 mg BID of tivantinib is recommended among Japanese patients with HCC regardless of CYP2C19 phenotype. PMID:25711511

  2. Ultrasound assisted-deep eutectic solvent based on emulsification liquid phase microextraction combined with microsample injection flame atomic absorption spectrometry for valence speciation of chromium(III/VI) in environmental samples.

    PubMed

    Yilmaz, Erkan; Soylak, Mustafa

    2016-11-01

    A new type of deep eutectic solvents (DESs) have been prepared and used as extraction solvents for ultrasound assisted-deep eutectic solvent based emulsification liquid phase microextraction method (UA-DES-ELPME) for the determination and speciation of total chromium, chromium(III) and chromium(VI). The chromium concentration in DES rich phase (extraction phase) was determined by using microsample injection flame atomic absorption spectrometer (FAAS). The detection limit (LOD), the quantification limit (LOQ), preconcentration factor and relative standard deviation were found as 5.5µgL(-1), 18.2µgL(-1), 20 and 6%, respectively. The accuracy of the developed method was evaluated by the analysis of water the certified reference materials (TMDA-53.3 Fortified environmental water and TMDA-54.4 Fortified Lake Water) and addition-recovery tests for water samples. PMID:27591663

  3. Pharmacokinetic Profile of Subcutaneous Testosterone Enanthate Delivered via a Novel, Prefilled Single‐Use Autoinjector: A Phase II Study

    PubMed Central

    Kaminetsky, Jed; Swerdloff, Ronald S.

    2015-01-01

    , Swerdloff RS. Pharmacokinetic profile of subcutaneous testosterone enanthate delivered via a novel, prefilled single‐use autoinjector: A phase II study. Sex Med 2015;3:263–273. PMID:26797061

  4. Climatological characteristics of deep and shallow precipitation clouds in summer over the Tibetan Plateau

    NASA Astrophysics Data System (ADS)

    Pan, Xiao; Fu, Yunfei

    2016-04-01

    The Tibetan Plateau (TP) is the highest plateau with complex terrain in the world, and it can affect the weather and climate of many regions through its mechanical and thermal effects. The precipitation types over TP are divided into deep (strong deep convection and weak deep convection) and shallow precipitations, according to the particular atmospheric vertical structures of TP. The climatological characteristics of the deep and shallow precipitations including their horizontal distributions, infrared signal characteristics of clouds, vertical structures, diurnal variations and local phase shifts over TP are investigated by utilizing combining measurements of the tropical rainfall measuring mission (TRMM) precipitation radar (PR) and visible and infrared scanner (VIRS) in summer from 1998 to 2007. The results show that the precipitation over TP is mainly in the form of weak deep convection, which occupies 67.8% of total rain, then followed by shallow precipitation with 26.4% and the strong deep convection with 5.8%. The clouds for strong deep convection and weak deep convection are mainly composed of ice and ice-water mixed particles, respectively. The vertical profiles of deep precipitations firstly increase, and then decrease from the storm top altitude to the ground level, with maximizing at about 7.5 km altitude. In contrast, the vertical profile of the shallow precipitation only has an increasing process. The deep precipitations have significant diurnal variations. The precipitation frequencies for strong deep convection and weak deep convection both peak at 16 local time (LT), while the rain intensities for them peak at 13 and 18 LST, respectively. Furthermore, the rain intensity for strong deep convection is characterized by a secondary peak at 00 LT. For the shallow precipitation, the diurnal variation is relatively weaker. The precipitation frequency and intensity both peak at 20 LT, presenting the characteristic of night rain. The diurnal variations of

  5. Fluxes of 230Th and 231Pa to the deep sea: implications for the interpretation of excess 230Th and 231Pa/ 230Th profiles in sediments

    NASA Astrophysics Data System (ADS)

    Yu, E.-F.; Francois, R.; Bacon, M. P.; Fleer, A. P.

    2001-09-01

    Analysis of samples obtained with deep-sea moored sediment traps deployed at 15 sites representing a wide range of oceanic conditions confirms that the flux of 230Th scavenged to the seafloor remains close to its production rate from the decay of 234U in the overlying water column, and generally validates the use of 230Th as a normalizing tool for paleoflux reconstruction. After correction for trapping efficiency, the flux of 230Th measured in the low flux regions amounts to 90±6% of the production rate, with the notable exceptions of one site near the Arabian Sea upwelling and one site in the Weddell Sea. A 230Th flux equivalent to 120% of the production rate was found in Panama Basin. Similar or more extensive scavenging of 230Th may be occurring at a Pacific margin site off California and south of the Polar Front, but these estimates are obscured by large errors on our trapping-efficiency estimates. In contrast, the flux of 231Pa and the 231Pa/ 230Th ratio can vary strongly with particle flux, following distinct trends in different oceanic basins. In the Atlantic Ocean, 231Pa fluxes and 231Pa/ 230Th are low and not sensitive to particle flux. This is because of the short residence time of deep water in this basin resulting from thermohaline circulation, which prevents the full development of lateral concentration gradients and full expression of boundary scavenging. In the Pacific Ocean, the sensitivity of 231Pa/ 230Th to particle flux is highest, reflecting the longer residence time of deep water. In the southern ocean, 231Pa/ 230Th ratios are invariably high, even when particle fluxes are low, reflecting the predominance of opal, which fractionates minimally between the two radionuclides. Interpretation of 231Pa/ 230Th recorded in sediments is thus complex. In the Atlantic, this ratio is better suited for recording past changes in the strength of thermohaline circulation. In the Pacific, it has the best potential for providing synoptic maps of past changes in

  6. Modeling migrations of slab-derived fluid in deep crust based on the considerations of solid phase deformation and wettability of solid-liquid system

    NASA Astrophysics Data System (ADS)

    Ohta, J.; Tokunaga, T.

    2012-12-01

    In deep crust, it is considered that the solid phase tends to keep its polycrystalline structure while it flows as highly-viscous fluid, and that the fluid phase flows through polycrystalline solid as porous flow. Here, the solid-framework is considered to change its bulk volume due to the changes of fluid volumetric fraction. In addition, wettability of solid-liquid system, which is expressed as solid-liquid dihedral angle, is also considered to affect internal pore structure. Thus, the solid-framework deformation and wettability of solid-liquid system are considered to influence fluid migration. In this research, we firstly constructed the permeability model to formulate a relationship among solid-liquid dihedral angle, fluid fraction, and permeability based on the energetic and textural considerations of grain boundary interface. The permeability and the fluid fraction under minimum interfacial energy condition were expressed as functions of solid-liquid dihedral angle from this model. Then, we found that permeability can be written as functions of the fluid fraction and the permeability under minimum interfacial energy condition. Secondly, we formulated the deformation of solid-framework and fluid flow through the deforming framework. The governing equations included solid bulk viscosity and solid shear viscosity as necessary parameters to describe the behavior. Based on the derived governing equations, the one-dimensional numerical simulations were conducted with different solid viscosities. From the results where solid bulk/shear viscosity was set to be 1020 Pa×s, intervals with relatively high fluid fraction were formed and the intervals showed attenuated fluctuation of their fluid volume fraction. On the other hand, the results where solid bulk/shear viscosity was set to be 1019 Pa×s did not show such fluctuation. The complex interaction among fluid fraction, permeability, and solid viscosity could contribute to the observed phenomena, and further

  7. Simulation of elution profiles in liquid chromatography-I: Gradient elution conditions, and with mismatched injection and mobile phase solvents.

    PubMed

    Jeong, Lena N; Sajulga, Ray; Forte, Steven G; Stoll, Dwight R; Rutan, Sarah C

    2016-07-29

    High-performance liquid chromatography (HPLC) simulators are effective method development tools. The goal of the present work was to design and implement a simple algorithm for simulation of liquid chromatographic separations that allows for characterization of the effect of injection solvent mismatch and injection solvent volume overload. The simulations yield full analyte profiles during solute migration and at elution, which enable a thorough physical understanding of the effects of method variables on chromatographic performance. The Craig counter-current distribution model (the plate model) is used as the basis for simulation, where a local retention factor is assigned for each spatial and temporal element within the simulation. The algorithm, which is an adaptation of an approach originally described by Czok and Guiochon (Ref. [10]), is sufficiently flexible to allow the use of either linear (e.g., Linear Solvent Strength Theory) or non-linear models of solute retention (e.g., Neue-Kuss (Ref. [36])). In this study, both types of models were used, one for simulating separations of a homologous series of alkylbenzenes, and the other for separations of selected amphetamines. The simulation program was validated first by comparison of simulated retention times and peak widths for five amphetamines to predictions obtained using linear solvent strength (LSS) theory, and to results from experimental separations of these compounds. The simulated retention times for the amphetamines agreed within 0.02% and 2.5% compared to theory and experiment, respectively. Secondly, the program was evaluated for simulating the case where there is a compositional mismatch between the mobile phase at the column inlet and the injection solvent (i.e., the sample matrix). This work involved alkylbenzenes, and retention time and peak width predictions from simulations were within 1.5 and 6.0% of experimental values, respectively, even without correction for extra-column dispersion. The

  8. Decrease and increase profile of Cu, Cr and Pb during stable phase of removal by duckweed (Lemna minor L.).

    PubMed

    Uçüncü, Esra; Tunca, Evren; Fikirdeşici, Seyda; Altindağ, Ahmet

    2013-01-01

    The present work details the decrease-increase profiles of Cu, Cr, and Pb by the aquatic plant Lemna minor. A mixture of these metals were utilized at different concentrations. Removal profiles of each metal was determined with water samples taken every 24 h for a 144 h period after the 48 h mark and was examined with correlation analysis. Removal profiles of Cr and Pb by L. minor from the mixture were observed to be highly similar with each other (r = 0.943). High proportion of Cr and Pb were removed compared to Cu and removal equations were defined with the aid of regression analysis. PMID:23488003

  9. Deep eutectic liquid organic salt as a new solvent for liquid-phase microextraction and its application in ligandless extraction and preconcentraion of lead and cadmium in edible oils.

    PubMed

    Karimi, Mehdi; Dadfarnia, Shayessteh; Shabani, Ali Mohammad Haji; Tamaddon, Fatemeh; Azadi, Davood

    2015-11-01

    Deep eutectic liquid organic salt was used as the solvent and a liquid phase microextraction (DES-LPME) combined with electrothermal atomic absorption spectrometry (ETAAS) was developed for separation, preconcentration and determination of lead and cadmium in edible oils. A 4:1 mixture of deep eutectic solvent and 2% nitric acid (200 µL) was added to an oil sample. The mixture was vortexed and transferred into a water bath at 50 °C and stirred for 5 minutes. After the extraction was completed, the phases were separated by centrifugation, and the enriched analytes in the deep eutectic solvent phase were determined by ETAAS. Under optimized extraction conditions and for an oil sample of 28 g, enhancement factors of 198 and 195 and limits of detection (defined as 3 Sb/m) of 8 and 0. 2 ng kg(-1) were achieved for lead and cadmium respectively. The method was successfully applied to the determination of lead and cadmium in various edible oils. PMID:26452873

  10. Deep Earthquakes.

    ERIC Educational Resources Information Center

    Frohlich, Cliff

    1989-01-01

    Summarizes research to find the nature of deep earthquakes occurring hundreds of kilometers down in the earth's mantle. Describes further research problems in this area. Presents several illustrations and four references. (YP)

  11. Reflective Moho beneath Qiangtang terrane, central Tibet, revealed by large explosive shot gathers along SinoProbe deep seismic reflection profile

    NASA Astrophysics Data System (ADS)

    Lu, Z.; Gao, R.; Li, H.; Li, Q.; Li, W.; Xiong, X.

    2013-12-01

    It is difficult to acquire deep seismic reflection data in central Tibet using the standard oil-industry acquisition parameters because of severe topography, rapid velocity and thickness variation of near-surface layer and strong seismic attenuation through the thickest crust of the Earth. Large explosive of 1000 kg seismic sources had been tentatively detonated in Qiangtang terrane and good quality data were acquired. The shot gather data showed clear Moho image in Qiangtang terrane. Moho was characterized by a sharp increase band in reflectivity at the base of the crust showed on shot records of the 1000-kg explosive. Moho reflection appeared at ~ 24 s TWT (~ 75.1 km)in the northmost Lhasa terrane and about 21 ~ 20 s TWT(65.7-62.6 km) beneath the Qiangtang terrane. We speculate that Moho get 9.4 km-12.5 km shallower from Lhasa to Qiangtang terrane rather than a 20 km offset. There is not obvious change of Moho depth across the Shung Hu suture.

  12. Northeastward growth of the Tibetan Plateau along the Tibet-Ordos transition zone-revealed from Liupanshan deep seismic reflection profile

    NASA Astrophysics Data System (ADS)

    Gao, R.; Wang, H.; Guo, X.; Li, W.; Li, H.; Hou, H.; Xiong, X.; Xu, X.; Liang, H.; Li, Q.

    2015-12-01

    Most previous studies of the Tibetan Plateau have focused on the processes of crustal thickening and subsequent lateral extrusion to account for the outward growth of the plateau. However, lithospheric structure across the tectonic boundaries of the Tibetan Plateau has not yet been fully imaged and, therefore, how geological structures evolved in association with the lateral expansion of the northeastern margin in particular remains unclear. Here, together with interpretation of regional geological and geophysical data, we employ a recently acquired 165 km-long deep seismic reflection image that crosses the Liupan shan (Fig. 1) northeastern flank of the Tibetan Plateau to show that crustal shortening, structural integrity, and topographic relief are strongly correlated. The resulting stratigraphic "architecture" suggests that crustal shortening is a primary driver for plateau uplift and expansion of northeastern Tibet and decoupled crustal deformation owing to differential structural integrity is accommodated during the subsequent northeastward growth of the plateau. Figure 1.Showing the seismic reflection line location and the topographic relief of the northeastern Tibetan Plateau and the western Ordos basin (KF: Kunlun Fault; LP Shan: Liupan Shan; HF: Haiyuan Fault; YTSF: Yangtongshan Fault; NSS-LSF: Niushou Shan-Luoshan Fault)XG Shan: Xiaoguan Shan; YJD: Yanjiadian Diorite; GS: Guanshan Shan; CCP: Caochuanpu; LS Complex: Longshan Complex)

  13. Carbon Storage in the Mid- to Deep- Upper Mantle Constrained by Phase Relations in the Fe-Ni-Cu-C-S system

    NASA Astrophysics Data System (ADS)

    Tsuno, K.; Dasgupta, R.

    2014-12-01

    Carbon is a key element for evolution of terrestrial planets as it has influence on the chemistry and habitability of surficial environment as well as impact on mantle processes such as partial melting and element mobility. Because mantle is arguably the largest reservoir of extractable carbon, the stable form of carbon in various mantle domains needs to be constrained. In the reduced, mid- to deep- upper mantle, the host of deep carbon is graphite/diamond and/or Fe-Ni-bearing alloy melt [1]. However, high solubility of carbon in Fe-Ni alloy melt [2] suggests that diamond saturation may be restricted only to C-rich mantle domains. But such suggestions do not take into account the role of sulfides, which must interact with alloy-carbon mantle subsystems. In order to constrain the stable forms of carbon in the reduced mantle where Ni-rich alloy is likely present [3], we explore the phase relations and C solubility in Ni-rich portion of the Fe-Ni-±Cu-C-S systems. Experiments were performed in a MgO capsule using a multi-anvil with six starting mixes (Ni/(Fe+Ni) wt. ratio of 0.50-0.61, 8-16 % wt.% S, 2.0-2.5 wt.% C, and 0-0.7 wt.% Cu) at 6-8 GPa and 800-1400 °C. Low-temperature runs for all starting mixes contain C-bearing, solid Fe-Ni alloy + alloy melt + graphite, and solid alloy-out boundary is constrained, for example, at 1000-1050 °C at 6 GPa and 900-1000 °C at 8 GPa for the S-rich starting mix. The carbon solubility in the alloy melt (0.8~2.1 wt.% at 8 GPa and 1400 °C) decreases with increasing S content from 8 to 24 wt.%, increasing pressure for S-rich (18-24 wt.%) melt, and decreasing Ni/(Fe+Ni) from 0.65 to 0.53. For a mantle with ~0.1 wt.% alloy (~250 km depth) [3], diamond is likely stable coexisting with an S-rich alloy melt for ≥10 ppm bulk C. This is owing to the influence of S, which suppresses the incorporation of C in the alloy melt to stabilize diamond. Our results thus imply that diamond is a stable form of carbon even in depleted mantle

  14. Solid and Dissolved Barium Profiles in Gas Hydrate Systems at Blake Ridge (ODP 164) and Peru Margin (ODP 201): Implications for Long-Term Carbon-Cycling in the Deep Biosphere.

    NASA Astrophysics Data System (ADS)

    Snyder, G. T.; Dickens, G. R.; Castellini, D. G.

    2003-12-01

    maximum of only 44 μ M at 250 m depth, followed by a steady decrease to 10 μ M at 750 mbsf. Although many pore water profiles are similar at the Blake Ridge and Peru Margin, the contrasting Ba profiles indicate some fundamental difference between the two locations. Below 250 m depth at the Blake Ridge, dissolved sulfate slowly rises and barium concentrations are at saturation with respect to barite. The decrease in barium concentrations along this interval may thus result from systematic seawater contamination during drilling, in which case the widely discussed profiles of other species in the Blake Ridge boreholes need to be corrected. Alternatively, unidentified processes in the deep biosphere (e.g., oxidation of sulfide-bearing minerals) release sulfate to pore waters, accounting for the depletion in dissolved barium concentrations. If this is the case, certain biotic processes at great depth are different at Blake Ridge and the Peru Margin.

  15. Analysis of expression profile of mce operon genes (mce1, mce2, mce3 operon) in different Mycobacterium tuberculosis isolates at different growth phases

    PubMed Central

    Singh, Pratibha; Katoch, V.M.; Mohanty, K.K.; Chauhan, Devendra Singh

    2016-01-01

    Background & objectives: Mycobacterium tuberculosis (M. tuberculosis) has four homologous mammalian cell entry (mce) operons (mce1-4) that encode exported proteins and have a possible role in the virulence mechanism of this pathogen. The expression of mce operon is considered to be complex and not completely understood. Although expression of mce operon at different in vitro growth phases has been studied earlier, its expression in different M. tuberculosis isolates under different growth phases is not yet studied. The present preliminary study was conducted on a limited number of isolates to know the trend of expression pattern of mce operon genes in different M. tuberculosis isolates under different growth stages. Methods: In this study, we monitored the transcriptional profile of selected mce operon genes (mce1A, mce1D, mce2A, mce2D, mce3A, mce3C) in different M. tuberculosis isolates (MDR1, MDR2, and sensitive isolate) at early exponential and stationary phases using real-time quantitative PCR. Results: The expression ratio of all selected mce operon genes in all M. tuberculosis isolates was reduced at the initial phase and increased substantially at a later phase of growth. Higher expression of mce1 operon genes was found in all M. tuberculosis isolates as compared to other mce operon genes (mce2 and mce3 operons) at stationary growth phase. Interpretation & conclusions: The higher expression of mce operon genes at stationary phase (as compared to early exponential phase) suggested growth phase dependent expression of mce operon genes. This indicated that the mce operon genes might have a role in M. tuberculosis survival and adaptation on the onset of adverse condition like stationary phase. Identification of differentially expressed genes will add to our understanding of the bacilli involved in adaptation to different growth conditions. PMID:27377506

  16. Identification and profiling of novel and conserved microRNAs during the flower opening process in Prunus mume via deep sequencing.

    PubMed

    Wang, Tao; Pan, Huitang; Wang, Jia; Yang, Weiru; Cheng, Tangren; Zhang, Qixiang

    2014-04-01

    MicroRNAs (miRNAs) are small (20-24 nucleotide) RNAs that are critical regulators of genes involved in diverse plant processes, including development, metabolism, abiotic stress and flowering. Prunus mume is a widely cultivated ornamental plant in East Asia that blooms in early spring, even at temperatures below 0 °C. While miRNAs involved in pistil development have been identified in P. mume, few studies have profiled miRNA expression patterns during flower opening. Here, we used high-throughput sequencing and bioinformatic analysis to identify and profile miRNAs that function during flower opening in P. mume. We identified 47 conserved miRNA sequences belonging to 25 miRNA families from 92 loci in P. mume, along with 33 novel miRNA sequences from 43 loci, including their complementary miRNA* strands. The expression levels of most differentially expressed miRNAs decreased during flower opening, while miR156e-f and miR477b were upregulated at the flowering stage. We predicted 88 target genes for conserved and novel miRNAs using computational analysis and annotated their functions. Seven target genes, encoding squamosa promoter binding protein-like (SPL) and auxin response factor (ARF), scarecrow-like transcription factor (SCL) and APETALA2-like transcription factors (AP2), were verified by 5'-RACE to be the targets of miR156, miR167, miR171 and miR172, respectively. Quantitative real-time PCR validated the expression of the miRNAs and seven target genes. The results help lay the foundation for investigating the roles of miRNAs in the blooming of P. mume. PMID:24343764

  17. Deciphering the Molecular Profile of Plaques, Memory Decline and Neuron Loss in Two Mouse Models for Alzheimer’s Disease by Deep Sequencing

    PubMed Central

    Bouter, Yvonne; Kacprowski, Tim; Weissmann, Robert; Dietrich, Katharina; Borgers, Henning; Brauß, Andreas; Sperling, Christian; Wirths, Oliver; Albrecht, Mario; Jensen, Lars R.; Kuss, Andreas W.; Bayer, Thomas A.

    2014-01-01

    One of the central research questions on the etiology of Alzheimer’s disease (AD) is the elucidation of the molecular signatures triggered by the amyloid cascade of pathological events. Next-generation sequencing allows the identification of genes involved in disease processes in an unbiased manner. We have combined this technique with the analysis of two AD mouse models: (1) The 5XFAD model develops early plaque formation, intraneuronal Aβ aggregation, neuron loss, and behavioral deficits. (2) The Tg4–42 model expresses N-truncated Aβ4–42 and develops neuron loss and behavioral deficits albeit without plaque formation. Our results show that learning and memory deficits in the Morris water maze and fear conditioning tasks in Tg4–42 mice at 12 months of age are similar to the deficits in 5XFAD animals. This suggested that comparative gene expression analysis between the models would allow the dissection of plaque-related and -unrelated disease relevant factors. Using deep sequencing differentially expressed genes (DEGs) were identified and subsequently verified by quantitative PCR. Nineteen DEGs were identified in pre-symptomatic young 5XFAD mice, and none in young Tg4–42 mice. In the aged cohort, 131 DEGs were found in 5XFAD and 56 DEGs in Tg4–42 mice. Many of the DEGs specific to the 5XFAD model belong to neuroinflammatory processes typically associated with plaques. Interestingly, 36 DEGs were identified in both mouse models indicating common disease pathways associated with behavioral deficits and neuron loss. PMID:24795628

  18. Identification and comparative analysis of the oriental river prawn (Macrobrachium nipponense) microRNA expression profile during hypoxia using a deep sequencing approach.

    PubMed

    Sun, Shengming; Fu, Hongtuo; Ge, Xianping; Zhu, Jian; Gu, Zhimin; Xuan, Fujun

    2016-03-01

    Hypoxia refers to a state of oxygen deficiency, which is observed frequently in aquaculture ponds. MicroRNAs (miRNAs) are small non-coding RNAs that are important effectors in regulating gene expression through posttranscriptional mechanisms. They are key elements in the response to hypoxia. The oriental river prawn (Macrobrachium nipponense) is an important commercial aquaculture species, and is sensitive to hypoxia. To date, there are no reports describing M. nipponense miRNAs. In this study, Solexa deep sequencing technology was used for high-throughput analysis of miRNAs in a small RNA library isolated from four M. nipponense tissues (gill, hepatopancreas, muscle and hemocytes). In total, 9,227,356 reads were obtained, 4,293,155 of which were related to 267 unique miRNAs, including 203 conserved and 64 prawn-specific miRNAs. Furthermore, miRNA features including length distribution and end variations were characterized. Annotation of targets revealed a broad range of biological processes and signal transduction pathways regulated by M. nipponense miRNAs. In addition, 880 co-expressed and 39 specific (25 normoxia-specific and 14 hypoxia-specific) miRNAs that may be involved in the response to hypoxia were confirmed using miRNA microarray analysis from the four prawn tissues combined. Real-time quantitative PCR (qPCR) analysis of eight miRNAs in the normoxia and hypoxia groups showed good concordance between the sequencing and qPCR data. This study provides the first large-scale identification and characterization of M. nipponense miRNAs and their potential targets, and represents a foundation for further characterization of their roles in the regulation of the diversity of hypoxia processes. PMID:26829004

  19. Deep sequencing analysis of HIV-1 reverse transcriptase at baseline and time of failure in patients receiving rilpivirine in the phase III studies ECHO and THRIVE.

    PubMed

    Van Eygen, Veerle; Thys, Kim; Van Hove, Carl; Rimsky, Laurence T; De Meyer, Sandra; Aerssens, Jeroen; Picchio, Gaston; Vingerhoets, Johan

    2016-05-01

    Minority variants (1.0-25.0%) were evaluated by deep sequencing (DS) at baseline and virological failure (VF) in a selection of antiretroviral treatment-naïve, HIV-1-infected patients from the rilpivirine ECHO/THRIVE phase III studies. Linkage between frequently emerging resistance-associated mutations (RAMs) was determined. DS (llIumina®) and population sequencing (PS) results were available at baseline for 47 VFs and time of failure for 48 VFs; and at baseline for 49 responders matched for baseline characteristics. Minority mutations were accurately detected at frequencies down to 1.2% of the HIV-1 quasispecies. No baseline minority rilpivirine RAMs were detected in VFs; one responder carried 1.9% F227C. Baseline minority mutations associated with resistance to other non-nucleoside reverse transcriptase inhibitors (NNRTIs) were detected in 8/47 VFs (17.0%) and 7/49 responders (14.3%). Baseline minority nucleoside/nucleotide reverse transcriptase inhibitor (NRTI) RAMs M184V and L210W were each detected in one VF (none in responders). At failure, two patients without NNRTI RAMs by PS carried minority rilpivirine RAMs K101E and/or E138K; and five additional patients carried other minority NNRTI RAMs V90I, V106I, V179I, V189I, and Y188H. Overall at failure, minority NNRTI RAMs and NRTI RAMs were found in 29/48 (60.4%) and 16/48 VFs (33.3%), respectively. Linkage analysis showed that E138K and K101E were usually not observed on the same viral genome. In conclusion, baseline minority rilpivirine RAMs and other NNRTI/NRTI RAMs were uncommon in the rilpivirine arm of the ECHO and THRIVE studies. DS at failure showed emerging NNRTI resistant minority variants in seven rilpivirine VFs who had no detectable NNRTI RAMs by PS. J. Med. Virol. 88:798-806, 2016. © 2015 Wiley Periodicals, Inc. PMID:26412111

  20. Grustal shortening in the Alpine Orogen: Results from deep seismic reflection profiling in the eastern Swiss Alps, Line NFP 20-east

    NASA Astrophysics Data System (ADS)

    Pfiffner, O. A.; Frei, W.; Valasek, P.; StäUble, M.; Levato, L.; Dubois, L.; Schmid, S. M.; Smithson, S. B.

    1990-12-01

    The deep crustal seismic line NFP 20-EAST crosses almost the entire Swiss Alps. Despite the complex geometry of the well-exposed nappe structure and the considerable axial plunge of some of the units, the Vibroseis survey yielded coherent reflections from several individually identifiable nappe contacts. In the northern part of the survey the Vibroseis data closely match the internal structure of the Helvetic nappes and the underlying autochthonous-parautochthonous Mesozoic sediments. On the northern flank of the Aar massif, an external basement uplift, these Mesozoic sediments seem to rise from a depth of approximately 7-8 km below sea level to the surface in a series of steps which is interpreted to represent crustal shortening achieved by a combination of folding and thrusting. In the southern part of the survey it was possible to image a number of thin slivers of Mesozoic carbonates pinched between slabs of Penninic basement nappes as well as nappe contacts between lithologically contrasting units. In addition, it seems that the Insubric fault zone, which marks the contact between the Penninic zone and the Southern Alps and which outcrops about 30 km to the south of the survey, shows up as steeply north dipping reflections. The lower European crust in the northern part of the survey is relatively transparent as opposed to the Adriatic lower crust, whose reflective nature may stem from shear zones associated with Mesozoic crustal stretching. The base of both the European and Adriatic crust coincides with a 1-s-thick band of laterally discontinuous reflections. This reflection Moho drops to greater depths going from the north toward the center of the Alpine chain, where it disappears with a steep southerly dip. The Moho reappears as a reflection band farther south. This Moho gap is situated above the lithospheric root and may be caused by perturbations related to subduction of lower crustal material. The crustal-scale structure obtained from the Vibroseis data

  1. Identification and profiling of growth-related microRNAs of the swimming crab Portunus trituberculatus by using Solexa deep sequencing.

    PubMed

    Ren, Xianyun; Cui, Yanting; Gao, Baoquan; Liu, Ping; Li, Jian

    2016-08-01

    MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate gene expression by post-transcriptional repression of mRNAs. The swimming crab Portunus trituberculatus is one of the most important crustacean species for aquaculture in China. However, to date no miRNAs have been reported to for modulating growth in P. trituberculatus. To investigate miRNAs involved in the growth of this species, we constructed six small RNA libraries for big individuals (BIs) and small individuals (SIs) from a highly inbred family. Six mixed RNA pools of five tissues (eyestalk, gill, heart, hepatopancreas, and muscle) were obtained. By aligning sequencing data with those for known miRNAs, a total of 404 miRNAs, including 339 known and 65 novel miRNAs, were identified from the six libraries. MiR-100 and miR-276a-3p were among the most prominent miRNA species. We identified seven differentially expressed miRNAs between the BIs and SIs, which were validated using real-time PCR. Preliminary analyzes of their putative target genes and GO and KEGG pathway analyzes showed that these differentially expressed miRNAs could play important roles in global transcriptional depression and cell differentiation of P. trituberculatus. This study reveals the first miRNA profile related to the body growth of P. trituberculatus, which would be particularly useful for crab breeding programs. PMID:27095170

  2. Proteomics and Deep Sequencing Comparison of Seasonally Active Venom Glands in the Platypus Reveals Novel Venom Peptides and Distinct Expression Profiles*

    PubMed Central

    Wong, Emily S. W.; Morgenstern, David; Mofiz, Ehtesham; Gombert, Sara; Morris, Katrina M.; Temple-Smith, Peter; Renfree, Marilyn B.; Whittington, Camilla M.; King, Glenn F.; Warren, Wesley C.; Papenfuss, Anthony T.; Belov, Katherine

    2012-01-01

    The platypus is a venomous monotreme. Male platypuses possess a spur on their hind legs that is connected to glands in the pelvic region. They produce venom only during the breeding season, presumably to fight off conspecifics. We have taken advantage of this unique seasonal production of venom to compare the transcriptomes of in- and out-of-season venom glands, in conjunction with proteomic analysis, to identify previously undiscovered venom genes. Comparison of the venom glands revealed distinct gene expression profiles that are consistent with changes in venom gland morphology and venom volumes in and out of the breeding season. Venom proteins were identified through shot-gun sequenced venom proteomes of three animals using RNA-seq-derived transcripts for peptide-spectral matching. 5,157 genes were expressed in the venom glands, 1,821 genes were up-regulated in the in-season gland, and 10 proteins were identified in the venom. New classes of platypus-venom proteins identified included antimicrobials, amide oxidase, serpin protease inhibitor, proteins associated with the mammalian stress response pathway, cytokines, and other immune molecules. Five putative toxins have only been identified in platypus venom: growth differentiation factor 15, nucleobindin-2, CD55, a CXC-chemokine, and corticotropin-releasing factor-binding protein. These novel venom proteins have potential biomedical and therapeutic applications and provide insights into venom evolution. PMID:22899769

  3. Alterations in SiRNA and MiRNA Expression Profiles Detected by Deep Sequencing of Transgenic Rice with SiRNA-Mediated Viral Resistance

    PubMed Central

    Wang, Xifeng; Liang, Chun

    2015-01-01

    RNA-mediated gene silencing has been demonstrated to serve as a defensive mechanism against viral pathogens by plants. It is known that specifically expressed endogenous siRNAs and miRNAs are involved in the self-defense process during viral infection. However, research has been rarely devoted to the endogenous siRNA and miRNA expression changes under viral infection if the resistance has already been genetically engineered in plants. Aiming to gain a deeper understanding of the RNA-mediated gene silencing defense process in plants, the expression profiles of siRNAs and miRNAs before and after viral infection in both wild type and transgenic anti-Rice stripe virus (RSV) rice plants were examined by small RNA high-throughput sequencing. Our research confirms that the newly generated siRNAs, which are derived from the engineered inverted repeat construct, is the major contributor of the viral resistance in rice. Further analysis suggests the accuracy of siRNA biogenesis might be affected when siRNAs machinery is excessively used in the transgenic plants. In addition, the expression levels of many known miRNAs are dramatically changed due to RSV infection on both wild type and transgenic rice plants, indicating potential function of those miRNAs involved in plant-virus interacting process. PMID:25559820

  4. Hollow fiber liquid-phase microextraction coupled with gas chromatography-flame ionization detection for the profiling of fatty acids in vegetable oils.

    PubMed

    Siang, Gan Hui; Makahleh, Ahmad; Saad, Bahruddin; Lim, Boey Peng

    2010-12-24

    The development of a two phase hollow fiber liquid-phase microextraction technique, followed by gas-chromatography-flame ionization detection (GC-FID) for the profiling of the fatty acids (FAs) (lauric, myristic, palmitic, stearic, palmitoleic, oleic, linoleic, linolenic and arachidic) in vegetable oils is described. Heptadecanoic acid methyl ester was used as the internal standard. The FAs were transesterified to their corresponding methyl esters prior to the extraction. Extraction parameters such as type of extracting solvent, temperature, extraction time, stirring speed and salt addition were studied and optimized. Recommended conditions were extraction solvent, n-tridecane; extraction time, 35 min; extraction temperature, ambient; without addition of salt. Enrichment factors varying from 37 to 115 were achieved. Calibration curves for the nine FAs were well correlated (r(2)>0.994) within the range of 10-5000 μg L(-1). The limit of detection (signal:noise, 3) was 4.73-13.21 ng L(-1). The method was successfully applied to the profiling of the FAs in palm oils (crude, olein, kernel, and carotino cooking oil) and other vegetable oils (soybean, olive, coconut, rice bran and pumpkin). The encouraging enrichments achieved offer an interesting option for the profiling of the minor and major FAs in palm and other vegetable oils. PMID:21081239

  5. Deep learning

    NASA Astrophysics Data System (ADS)

    Lecun, Yann; Bengio, Yoshua; Hinton, Geoffrey

    2015-05-01

    Deep learning allows computational models that are composed of multiple processing layers to learn representations of data with multiple levels of abstraction. These methods have dramatically improved the state-of-the-art in speech recognition, visual object recognition, object detection and many other domains such as drug discovery and genomics. Deep learning discovers intricate structure in large data sets by using the backpropagation algorithm to indicate how a machine should change its internal parameters that are used to compute the representation in each layer from the representation in the previous layer. Deep convolutional nets have brought about breakthroughs in processing images, video, speech and audio, whereas recurrent nets have shone light on sequential data such as text and speech.

  6. Deep learning.

    PubMed

    LeCun, Yann; Bengio, Yoshua; Hinton, Geoffrey

    2015-05-28

    Deep learning allows computational models that are composed of multiple processing layers to learn representations of data with multiple levels of abstraction. These methods have dramatically improved the state-of-the-art in speech recognition, visual object recognition, object detection and many other domains such as drug discovery and genomics. Deep learning discovers intricate structure in large data sets by using the backpropagation algorithm to indicate how a machine should change its internal parameters that are used to compute the representation in each layer from the representation in the previous layer. Deep convolutional nets have brought about breakthroughs in processing images, video, speech and audio, whereas recurrent nets have shone light on sequential data such as text and speech. PMID:26017442

  7. Reversed-phase high-performance liquid chromatography-electrospray mass spectrometry profiling of transgenic and non-transgenic maize for cultivar characterization.

    PubMed

    López, Ma Concepción García; Garcia-Cañas, Virginia; Alegre, Ma Luisa Marina

    2009-10-23

    A reversed-phase high-performance liquid chromatography-electrospray mass spectrometry (RP-HPLC-ESI-MS (ion trap)) method is developed, for the first time, for profiling transgenic and non-transgenic maize with the aim of cultivar characterization. To optimize chromatographic conditions the following parameters were studied: column, gradient, and ion-pairing reagent. Moreover, the influence in the MS signal of the variation of the capillary voltage and the accumulated ions in the trap was also studied. The developed method was applied to the profiling of different protein fractions (albumin, globulin, prolamin, and glutelin) isolated from Bt transgenic and non-transgenic maize cultivars. Moreover, different maize samples, namely, maize cultivars from different geographical origins (USA, Canada, France, and Spain), transgenic maize samples with certified GMO content, and three transgenic Bt maize cultivars with their isogenic non-transgenic counterparts (Aristis Bt vs. Aristis, PR33P67 vs. PR33P66, and DKC6575 vs. Tietar) were profiled by the developed method. Mass spectra obtained for certain peaks in the maize cultivars studied resulted, in some occasions, useful for cultivar characterization and differentiation. The comparison of UV and MS profiles and mass spectra corresponding to the protein fractions with those of the whole seeds enabled the assignment of some peaks. PMID:19748098

  8. Deep Sequencing–Based Transcriptome Profiling Reveals Comprehensive Insights into the Responses of Nicotiana benthamiana to Beet necrotic yellow vein virus Infections Containing or Lacking RNA4

    PubMed Central

    Fan, Huiyan; Sun, Haiwen; Wang, Ying; Zhang, Yongliang; Wang, Xianbing; Li, Dawei; Yu, Jialin; Han, Chenggui

    2014-01-01

    Background Beet necrotic yellow vein virus (BNYVV), encodes either four or five plus-sense single stranded RNAs and is the causal agent of sugar beet rhizomania disease, which is widely distributed in most regions of the world. BNYVV can also infect Nicotiana benthamiana systemically, and causes severe curling and stunting symptoms in the presence of RNA4 or mild symptoms in the absence of RNA4. Results Confocal laser scanning microscopy (CLSM) analyses showed that the RNA4-encoded p31 protein fused to the red fluorescent protein (RFP) accumulated mainly in the nuclei of N. benthamiana epidermal cells. This suggested that severe RNA4-induced symptoms might result from p31-dependent modifications of the transcriptome. Therefore, we used next-generation sequencing technologies to analyze the transcriptome profile of N. benthamiana in response to infection with different isolates of BNYVV. Comparisons of the transcriptomes of mock, BN3 (RNAs 1+2+3), and BN34 (RNAs 1+2+3+4) infected plants identified 3,016 differentially expressed transcripts, which provided a list of candidate genes that potentially are elicited in response to virus infection. Our data indicate that modifications in the expression of genes involved in RNA silencing, ubiquitin-proteasome pathway, cellulose synthesis, and metabolism of the plant hormone gibberellin may contribute to the severe symptoms induced by RNA4 from BNYVV. Conclusions These results expand our understanding of the genetic architecture of N. benthamiana as well as provide valuable clues to identify genes potentially involved in resistance to BNYVV infection. Our global survey of gene expression changes in infected plants reveals new insights into the complicated molecular mechanisms underlying symptom development, and aids research into new strategies to protect crops against viruses. PMID:24416380

  9. Identification and Comparative Profiling of miRNAs in an Early Flowering Mutant of Trifoliate Orange and Its Wild Type by Genome-Wide Deep Sequencing

    PubMed Central

    Li, Wen-Yang; Guo, Wen-Wu; Deng, Xiu-Xin; Hu, Chun-Gen; Zhang, Jin-Zhi

    2012-01-01

    MicroRNAs (miRNAs) are a new class of small, endogenous RNAs that play a regulatory role in various biological and metabolic processes by negatively affecting gene expression at the post-transcriptional level. While the number of known Arabidopsis and rice miRNAs is continuously increasing, information regarding miRNAs from woody plants such as citrus remains limited. Solexa sequencing was performed at different developmental stages on both an early flowering mutant of trifoliate orange (precocious trifoliate orange, Poncirus trifoliata L. Raf.) and its wild-type in this study, resulting in the obtainment of 141 known miRNAs belonging to 99 families and 75 novel miRNAs in four libraries. A total of 317 potential target genes were predicted based on the 51 novel miRNAs families, GO and KEGG annotation revealed that high ranked miRNA-target genes are those implicated in diverse cellular processes in plants, including development, transcription, protein degradation and cross adaptation. To characterize those miRNAs expressed at the juvenile and adult development stages of the mutant and its wild-type, further analysis on the expression profiles of several miRNAs through real-time PCR was performed. The results revealed that most miRNAs were down-regulated at adult stage compared with juvenile stage for both the mutant and its wild-type. These results indicate that both conserved and novel miRNAs may play important roles in citrus growth and development, stress responses and other physiological processes. PMID:22952759

  10. Delipidation-based solid-phase extraction pretreatment technique for plasma broad-coverage metabolomic profiling to reveal the potential pathogenesis of yeast-induced fever in rats.

    PubMed

    Zhang, Zhixin; Qin, Lingling; Guo, Mingxing; Gao, Shanshan; Zhang, Qingqing; Wang, Qing; Lu, Zhiwei; Zhao, Huizhen; Liu, Yuehong; Wang, Meiling; Fu, Shuang; Bai, Xu; Gao, Xiaoyan

    2016-07-01

    During the process of metabolomics profiling by using ultra high performance liquid chromatography coupled with time-of flight mass spectrometry, blood sample pretreatment is a crucial step for biomarker discovery. Herein, in order to prevent the potential loss of metabolites and ion suppression phenomena caused by the proteins and phospholipids contained in blood fluids, a delipidation-based solid-phase extraction pretreatment technique for plasma broad-coverage metabolomic profiling was performed. This technique can be summarized as a single extraction, a single elution of solid-phase extraction plate, followed by four times measuring with electrospray ionization in positive and negative ion mode, respectively. This approach significantly increased the number of features detected in plasma, and 1572 features in positive mode and 1352 features in negative mode were detected, respectively. Besides, the stability and repeatability of the approach were greatly improved. For these advantages, the approach was employed to elucidate the potential pathogenesis of yeast-induced fever in rats. The biomarkers associated with the pathogenesis of fever were shown to be related to amino acids metabolism and lipid metabolism. The delipidation-based solid-phase extraction pretreatment approach can provide a useful tool to reveal the pathological mechanisms of such systemic pathological process. PMID:27173137

  11. Comparative profiling of differentially expressed microRNAs between the follicular and luteal phases ovaries of goats.

    PubMed

    Zhu, Long; Chen, Tao; Sui, Menghua; Han, Chunyang; Fang, Fugui; Ma, Yuehui; Chu, Mingxing; Zhang, Xiaorong; Liu, Cuiyan; Ling, Yinghui

    2016-01-01

    To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total, 320 known miRNAs were co-expressed in the two phases, 339 and 353 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 45 novel miRNAs were co-expressed in the two phases, 70 and 94 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNA in the two phases, and mir-159 was the highest expressed significantly different novel miRNA in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. GO annotation and KEGG pathway analysis were applied to analyze the target genes of differentially expressed miRNAs detected in the two phases. The results will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries. PMID:27610292

  12. Crustal structure of the Celtic Sea and western approaches from gravity data and deep seismic profiles: Constraints on the formation of continental basins

    NASA Astrophysics Data System (ADS)

    Sibuet, Jean-Claude; Dyment, Jerome; Bois, Christian; Pinet, Bertrand; Ondreas, Helene

    1990-07-01

    A free-air anomaly map of the Celtic Sea and the Western Approaches is presented including all available marine gravimetric data and Bouguer anomalies on land. N60° features are predominant in the area. They correspond to the N60° so-called Caledonian grain which played a major role in the Variscan orogeny and in the formation of the Permo-Triassic sedimentary basins. The fundamental new point which comes out from this gravimetric map is the existence of a closely spaced pattern of N105° features which we regard as late Carboniferous transpressional structures. In the eastern pan of the Celtic Sea, thrusts are in the same N105° direction. West of 6°W longitude, however, folds and thrusts trend preferentially in a N60° direction, following the older grain. There, the N105° lineaments may be regarded as dextral shear structures with a possible compressive component. The Hercynian shortening of the Celtic Sea domain, located between the Variscan Front and the Lizard suture, resulted from the displacement, along the N160° direction of the two adjacent rigid domains. North of the Variscan Front, the N105° and N135° features were possibly active in more recent times. Then, both the N60° and N105° trends controlled the formation of Permo-Triassic basins, the N105° directions being transfer faults. Two-dimensional gravity modelling of the SWAT profiles in the Celtic Sea shows that the base of the reflective lower crust may be associated with the Moho discontinuity everywhere in the Celtic Sea except below the Saint George's Channel basin. The lower crust does not change significantly in depth and thickness beneath the Permo-Triassic basins. None of the available models simply satisfies such conditions. They all require at least a modification of the geometry of the lower crust after the formation of the Celtic Sea basins. Nevertheless, if the simple shear model can be ruled out mainly from structural arguments, the uniform stretching and to a lesser degree the

  13. Direct observation of solid-phase adsorbate concentration profile in powdered activated carbon particle to elucidate mechanism of high adsorption capacity on super-powdered activated carbon.

    PubMed

    Ando, Naoya; Matsui, Yoshihiko; Matsushita, Taku; Ohno, Koichi

    2011-01-01

    Decreasing the particle size of powdered activated carbon (PAC) by pulverization increases its adsorption capacities for natural organic matter (NOM) and polystyrene sulfonate (PSS, which is used as a model adsorbate). A shell adsorption mechanism in which NOM and PSS molecules do not completely penetrate the adsorbent particle and instead preferentially adsorb near the outer surface of the particle has been proposed as an explanation for this adsorption capacity increase. In this report, we present direct evidence to support the shell adsorption mechanism. PAC particles containing adsorbed PSS were sectioned with a focused ion beam, and the solid-phase PSS concentration profiles of the particle cross-sections were directly observed by means of field emission-scanning electron microscopy/energy-dispersive X-ray spectrometry (FE-SEM/EDXS). X-ray emission from sulfur, an index of PSS concentration, was higher in the shell region than in the inner region of the particles. The X-ray emission profile observed by EDXS did not agree completely with the solid-phase PSS concentration profile predicted by shell adsorption model analysis of the PSS isotherm data, but the observed and predicted profiles were not inconsistent when the analytical errors were considered. These EDXS results provide the first direct evidence that PSS is adsorbed mainly in the vicinity of the external surface of the PAC particles, and thus the results support the proposition that the increase in NOM and PSS adsorption capacity with decreasing particle size is due to the increase in external surface area on which the molecules can be adsorbed. PMID:20851447

  14. CO2 dynamics in the Amargosa Desert: Fluxes and isotopic speciation in a deep unsaturated zone

    USGS Publications Warehouse

    Walvoord, M.A.; Striegl, R.G.; Prudic, D.E.; Stonestrom, D.A.

    2005-01-01

    Natural unsaturated-zone gas profiles at the U.S. Geological Survey's Amargosa Desert Research Site, near Beatty, Nevada, reveal the presence of two physically and isotopically distinct CO2 sources, one shallow and one deep. The shallow source derives from seasonally variable autotrophic and heterotrophic respiration in the root zone. Scanning electron micrograph results indicate that at least part of the deep CO2 source is associated with calcite precipitation at the 110-m-deep water table. We use a geochemical gas-diffusion model to explore processes of CO2 production and behavior in the unsaturated zone. The individual isotopic species 12CO2, 13CO2, and 14CO2 are treated as separate chemical components that diffuse and react independently. Steady state model solutions, constrained by the measured PCO2 ??13C (in CO2), and ??14C (in CO2) profiles, indicate that the shallow CO2 source from root and microbial respiration composes ???97% of the annual average total CO2 production at this arid site. Despite the small contribution from deep CO2 production amounting to ???0.1 mol m-2 yr-1, upward diffusion from depth strongly influences the distribution of CO2 and carbon isotopes in the deep unsaturated zone. In addition to diffusion from deep CO2 production, 14C exchange with a sorbed CO2 phase is indicated by the modeled ??14C profiles, confirming previous work. The new model of carbon-isotopic profiles provides a quantitative approach for evaluating fluxes of carbon under natural conditions in deep unsaturated zones.

  15. 30 CFR 203.43 - To which production do I apply the RSV earned from qualified deep wells or qualified phase 1...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... first qualified well that earns your lease the RSV begins production (other than test production). (2... well when production begins in August 2008. The first well earned an RSV of 25 BCF (see § 203.41(a)(1... second well had begun production in August 2009, it would not be a qualified deep well because it...

  16. Deep Lysimeter

    DOEpatents

    Hubbell, Joel M.; Sisson, James B.

    2004-06-01

    A deep lysimeter including a hollow vessel having a chamber, a fill conduit extending into the chamber through apertures, a semi-permeable member mounted on the vessel and in fluid communication with the fill conduit, and a line connection for retrieving the lysimeter.

  17. Is the solvation parameter model or its adaptations adequate to account for ionic interactions when characterizing stationary phases for drug impurity profiling with supercritical fluid chromatography?

    PubMed

    Galea, Charlene; West, Caroline; Mangelings, Debby; Vander Heyden, Yvan

    2016-06-14

    Nine commercially available polar and aromatic stationary phases were characterized under supercritical fluid chromatographic (SFC) conditions. Retention data of 64 pharmaceutical compounds was acquired to generate models based on the linear solvation energy relationship (LSER) approach. Previously, adaptation of the LSER model was done in liquid chromatography by the addition of two solute descriptors to describe the influence of positive (D(+)) and negative (D(-)) charges on the retention of ionized compounds. In this study, the LSER models, with and without the ionization terms for acidic and basic solutes, were compared. The improved fits obtained for the modified models support inclusion of the D(+) and D(-) terms for pharmaceutical compounds. Moreover, the statistical significance of the new terms in the models indicates the importance of ionic interactions in the retention of pharmaceutical compounds in SFC. However, unlike characterization through the retention profiles, characterization of the stationary phases by modelling never explains the retention variance completely and thus seems less appropriate. PMID:27181639

  18. Deep level traps in GaN LEDs grown by metal organic vapour phase epitaxy on an 8 inch Si(111) substrate

    NASA Astrophysics Data System (ADS)

    Nguyen, Xuan Sang; Goh, Xuan Long; Zhang, Li; Zhang, Zeng; Arehart, Aaron R.; Ringel, Steven A.; Fitzgerald, Eugene A.; Chua, Soo Jin

    2016-06-01

    Deep level traps present in GaN LED grown on 8 in. Si substrate were revealed by deep level transient spectroscopy (DLTS). One electron trap located at E C ‑ 0.7 eV was revealed in the n-GaN barrier layer. Two electron traps and one hole trap were observed in the p-GaN layer. They are located at E C ‑ 0.60 eV, E C ‑ 0.79 eV and E V + 0.70 eV. The total trap density in both the n-GaN barrier layer and the p-GaN layer of the LED is in order of 1014 cm‑3, which is comparable with that found in GaN epi-layer grown on sapphire.

  19. Metabolic profile of mephedrone: Identification of nor-mephedrone conjugates with dicarboxylic acids as a new type of xenobiotic phase II metabolites.

    PubMed

    Linhart, Igor; Himl, Michal; Židková, Monika; Balíková, Marie; Lhotková, Eva; Páleníček, Tomáš

    2016-01-01

    Metabolic profile of mephedrone (4-methylmethcathinone, 4-MMC), a frequently abused recreational drug, was determined in rats in vivo. The urine of rats dosed with a subcutaneous bolus dose of 20mg 4-MMC/kg was analysed by LC/MS. Ten phase I and five phase II metabolites were identified by comparison of their retention times and MS(2) spectra with those of authentic reference standards and/or with the MS(2) spectra of previously identified metabolites. The main metabolic pathway was N-demethylation leading to normephedrone (4-methylcathinone, 4-MC) which was further conjugated with succinic, glutaric and adipic acid. Other phase I metabolic pathways included oxidation of the 4-methyl group, carbonyl reduction leading to dihydro-metabolites and ω-oxidation at the position 3'. Five of the metabolites detected, namely, 4-carboxynormephedrone (4-carboxycathinone, 4-CC), 4-carboxydihydronormephedrone (4-carboxynorephedrine, 4-CNE), hydroxytolyldihydro-normephedrone (4-hydroxymethylnorephedrine, 4-OH-MNE) and conjugates of 4-MC with glutaric and adipic acid, have not been reported as yet. The last two conjugates represent a novel, hitherto unexploited, type of phase II metabolites in mammals together with an analogous succinic acid conjugate of 4-MC identified by Pozo et al. (2015). These conjugates might be potentially of great importance in the metabolism of other psychoactive amines. PMID:26541208

  20. Menstrual cycle phase at the time of rape does not affect recovery of semen or amplification of STR profiles of a suspect in vaginal swabs.

    PubMed

    Cerdas, Loreley; Herrera, Fabiola; Arrieta, Glenn; Morelli, Concepción; Álvarez, Karla; Gómez, Aarón

    2016-02-01

    The effect of women menstrual cycle on the forensic analysis of rapes was studied in a random group of 170 victims aged among 10 and 51 years. Participants were grouped according to the day of the menstrual cycle in which they were at the moment of the assault. From each participant, samples of vaginal fluid were taken and analyzed for sperm cells, p30 protein, total human DNA and human male DNA. Moreover, amplification of suspect's autosomal STR and Y-STR was attempted. Suspects' autosomal STR profiles were obtained from 92 of the 101 samples in which spermatozoa were found; and Y-STR haplotype was obtained in 1 of the 9 samples where autosomal STR profiles of a male were not obtained. On the other hand, Y-STR haplotypes were obtained in 2 of the 21 samples negative for sperm cells but positive for p30 protein. Y-STR haplotypes were also obtained in 11 of the 48 samples negative for sperm cells and p30 protein. It was found that groups of participants did not differ on the recovery of sperm cells from the vaginal swabs, quantification of suspect's DNA or amplification of their STR profiles. It is concluded that the menstrual cycle phase at the moment of the sexual assault does not affect the main outcomes of the forensic investigation of rapes. PMID:26734988

  1. Variable neutron star free precession in Hercules X-1 from evolution of RXTE X-ray pulse profiles with phase of the 35-d cycle

    NASA Astrophysics Data System (ADS)

    Postnov, K.; Shakura, N.; Staubert, R.; Kochetkova, A.; Klochkov, D.; Wilms, J.

    2013-10-01

    Accretion of matter on to the surface of a freely precessing neutron star (NS) with a complex non-dipole magnetic field can explain the change of X-ray pulse profiles of Her X-1 observed by RXTE with the phase of the 35-d cycle. We demonstrate this using all available measurements of X-ray pulse profiles in the 9-13 keV energy range obtained with the RXTE/Proportional Counter Array (PCA). The measured profiles guided the elaboration of a geometrical model and the definition of locations of emitting poles, arcs and spots on the NS surface which satisfactorily reproduce the observed pulse profiles and their dependence on free precession phase. We have found that the observed trend of the times of the 35-d turn-ons on the O-C diagram, which can be approximated by a collection of consecutive linear segments around the mean value, can be described by our model by assuming a variable free precession period, with a fractional period change of about a few per cent. Under this assumption and using our model, we have found that the times of phase zero of the NS free precession (which we identify with the maximum separation of the brightest spot on the NS surface with the NS spin axis) occur about 1.6 d after the mean turn-on times inside each `stable' epoch, producing a linear trend on the O-C diagram with the same slope as the observed times of turn-ons. We propose that the 2.5 per cent changes in the free precession period that occur on time scales of several to tens of 35-d cycles can be related to wandering of the principal inertia axis of the NS body due to variations in the patterns of accretion on to the NS surface. The closeness of periods of the disc precession and the NS free precession can be explained by the presence of a synchronization mechanism in the system, which modulates the dynamical interaction of the gas streams and the accretion disc with the NS free precession period.

  2. STIMULATION TECHNOLOGIES FOR DEEP WELL COMPLETIONS

    SciTech Connect

    Stephen Wolhart

    2003-06-01

    The Department of Energy (DOE) is sponsoring a Deep Trek Program targeted at improving the economics of drilling and completing deep gas wells. Under the DOE program, Pinnacle Technologies is conducting a project to evaluate the stimulation of deep wells. The objective of the project is to assess U.S. deep well drilling & stimulation activity, review rock mechanics & fracture growth in deep, high pressure/temperature wells and evaluate stimulation technology in several key deep plays. Phase 1 was recently completed and consisted of assessing deep gas well drilling activity (1995-2007) and an industry survey on deep gas well stimulation practices by region. Of the 29,000 oil, gas and dry holes drilled in 2002, about 300 were drilled in the deep well; 25% were dry, 50% were high temperature/high pressure completions and 25% were simply deep completions. South Texas has about 30% of these wells, Oklahoma 20%, Gulf of Mexico Shelf 15% and the Gulf Coast about 15%. The Rockies represent only 2% of deep drilling. Of the 60 operators who drill deep and HTHP wells, the top 20 drill almost 80% of the wells. Six operators drill half the U.S. deep wells. Deep drilling peaked at 425 wells in 1998 and fell to 250 in 1999. Drilling is expected to rise through 2004 after which drilling should cycle down as overall drilling declines.

  3. Evaluation of Free To Grow, Phase II: Detailed Profile of the Free To Grow Project in California. Final Report.

    ERIC Educational Resources Information Center

    Harrington, Mary

    The Free to Grow pilot project, developed by the Charles R. Drew University of Medicine and Science and its Head Start project, operated between 1994 and 1999. Following a 2-year planning and development stage, 5 project sites went on to complete the 3-year implementation phase in California, Colorado, Kentucky, New York, and Puerto Rico; the…

  4. Biological profiling of the ToxCast Phase II Chemical Library in Primary Human Cell Co-Culture Systems

    EPA Science Inventory

    The U.S. EPA’s ToxCast research project was developed to address the need for high-throughput testing of chemicals and a pathway-based approach to hazard screening. Phase I of ToxCast tested over 300 unique compounds (mostly pesticides and antimicrobials). With the addition of Ph...

  5. Health-Related Intensity Profiles of Physical Education Classes at Different Phases of the Teaching/Learning Process

    ERIC Educational Resources Information Center

    Bronikowski, Michal; Bronikowska, Malgorzata; Kantanista, Adam; Ciekot, Monika; Laudanska-Krzeminska, Ida; Szwed, Szymon

    2009-01-01

    Study aim: To assess the intensities of three types of physical education (PE) classes corresponding to the phases of the teaching/learning process: Type 1--acquiring and developing skills, Type 2--selecting and applying skills, tactics and compositional principles and Type 3--evaluating and improving performance skills. Material and methods: A…

  6. HST Multicolor (255-1042 nm) Photometry of Saturn's Main Rings. 1; Radial Profiles, Phase and Opening Angle Variations, and Regional Spectra

    NASA Technical Reports Server (NTRS)

    Cuzzi, Jeffrey N.; French, Richard G.; Dones, Luke; DeVincenzi, Donald (Technical Monitor)

    2001-01-01

    The main rings of Saturn were observed with the Planetary Camera of the WFPC2 instrument on the Hubble Space Telescope (HST) from September 1996 to August 2000 as the'ring opening angle to Earth and Sun increased from 4 deg to 24 deg, with a spread of phase angles between 0.3 deg and 6 deg at each opening angle. The rings were routinely observed in the five HST wideband UBVRI filters (F336W, F439W, F555W, F675W, and F814W) and occasionally in the F255W, F785LP, and F1042M filters. The emphasis in this series of papers will be on radial color (implying compositional) variations. In this first paper we describe the analysis technique and calibration procedure, note revisions in a previously published Voyager ring color data analysis, and present new results based on over 100 HST images. In the 300-600 nm spectral range where the rings are red, the 555nm/336nm ratio increases by about 14% as the phase angle increases from 0.3 deg to 6 deg. This effect, never reported previously for the rings, is significantly larger than the phase reddening which characterizes other icy objects, primarily because of the redness of the rings. However, there is no discernible tendency for color to vary with ring opening angle at a given phase angle, and there is no phase variation of color where the spectrum is flat. We infer from this combination of facts that multiple intraparticle scattering, either in a regolith or between facets of an unusually rough surface, is important in these geometries, but that multiple interparticle scattering in a vertically extended layer is not. Voyager color ratios at a phase angle of 14 deg are compatible with this trend, but calibration uncertainties prevent their use in quantitative modeling. Overall ring-average spectra are compatible with those of earlier work within calibration uncertainties, but ring spectra vary noticeably with region. We refine and subdivide the regions previously defined by others. The variation seen between radial profiles of

  7. Optimized Analytical Procedures for the Untargeted Metabolomic Profiling of Human Urine and Plasma by Combining Hydrophilic Interaction (HILIC) and Reverse-Phase Liquid Chromatography (RPLC)-Mass Spectrometry.

    PubMed

    Contrepois, Kévin; Jiang, Lihua; Snyder, Michael

    2015-06-01

    Profiling of body fluids is crucial for monitoring and discovering metabolic markers of health and disease and for providing insights into human physiology. Since human urine and plasma each contain an extreme diversity of metabolites, a single liquid chromatographic system when coupled to mass spectrometry (MS) is not sufficient to achieve reasonable metabolome coverage. Hydrophilic interaction liquid chromatography (HILIC) offers complementary information to reverse-phase liquid chromatography (RPLC) by retaining polar metabolites. With the objective of finding the optimal combined chromatographic solution to profile urine and plasma, we systematically investigated the performance of five HILIC columns with different chemistries operated at three different pH (acidic, neutral, basic) and five C18-silica RPLC columns. The zwitterionic column ZIC-HILIC operated at neutral pH provided optimal performance on a large set of hydrophilic metabolites. The RPLC columns Hypersil GOLD and Zorbax SB aq were proven to be best suited for the metabolic profiling of urine and plasma, respectively. Importantly, the optimized HILIC-MS method showed excellent intrabatch peak area reproducibility (CV < 12%) and good long-term interbatch (40 days) peak area reproducibility (CV < 22%) that were similar to those of RPLC-MS procedures. Finally, combining the optimal HILIC- and RPLC-MS approaches greatly expanded metabolome coverage with 44% and 108% new metabolic features detected compared with RPLC-MS alone for urine and plasma, respectively. The proposed combined LC-MS approaches improve the comprehensiveness of global metabolic profiling of body fluids and thus are valuable for monitoring and discovering metabolic changes associated with health and disease in clinical research studies. PMID:25787789

  8. Phase relations of CaAl 4Si 2O 11 at high-pressure and high-temperature with implications for subducted continental crust into the deep mantle

    NASA Astrophysics Data System (ADS)

    Zhai, Shuangmeng; Ito, Eiji

    2008-04-01

    Phase relations of the CAS phase, CaAl 4Si 2O 11, have been investigated at pressures of 10-23 GPa and at temperatures of 1000-1600 °C by means of the quench method using the Kawai-type high-pressure apparatus. An assemblage of grossular (Gr) + corundum (Cor) + kyanite (Ky) is stable up to about 10 GPa and at the temperatures examined. The assemblage converts into the CAS phase at 1600 °C and higher pressures. Below 1450 °C, however, the assemblage first changes to that of Gr + Cor + stishovite (St), corresponding to the dissociation of Ky into Cor + St, and then converts to the CAS phase with increasing pressure. The Gr + Cor + St-CAS boundary has a negative d P/d T slope, and the Gr + Ky + Cor-CAS boundary has a near horizontal slope. The triple point at which Gr, Cor, Ky, St and the CAS phase coexist is located at around 11.5 GPa and 1450 °C. Stability of the CAS phase up to 23 GPa and 1600 °C indicates that the CAS phase is an important host of Al and Ca in the continental crust subducted into deep mantle. Comparison of the densities between the pyrolitic mantle and the subducted continental crust indicates that the continental crust provides a plunging force to the slab subducted into the upper mantle and transition zone, whereas the continental crust in turn gives a buoyancy in the lower mantle as the consequence of the substantial changes in mineral assemblage in both the continental crust and pyrolitic mantle through the 660 km discontinuity. The formation of the CAS phase plays an important role in changing the density of the subducting continental crust.

  9. Antibodies against acute phase proteins and their functions in the pathogenesis of disease: a collective profile of 25 different antibodies.

    PubMed

    Lakota, Katja; Zigon, Polona; Mrak-Poljsak, Katjusa; Rozman, Blaz; Shoenfeld, Yehuda; Sodin-Semrl, Snezna

    2011-10-01

    The acute phase response is a defense system in which the innate immune response is activated following injury or infection. Positive and negative acute phase proteins (APPs) are crucial for protecting the host organism, as well as returning it to homeostatic levels, the first with elevated concentrations and the latter with decreased concentrations during the acute phase. Reports about the presence of antibodies against APPs are known, however their individual, as well as potentially collective, pathological or physiological roles are still emerging. Some of these autoantibodies are specifically connected with diseases (such as pancreatic secretory trypsin inhibitor and C3, C4 nephritic factors), while others have been reported as natural antibodies. The persistent presence (even if only minor) of autoantibodies in healthy blood donors indicates an overlapping category of autoantibodies, which could become pathogenic, depending on the autoantibody characteristics such as avidity, epitope specificity, changes in the microenvironment leading to different oxidative status and others. This review uses the novel approach of studying the overall autoantibody population against APPs, their functions and connections to diseases. The primary function of autoantibodies against APPs (anti-APPs) is thought to promote their clearance, however autoantibodies against negative APPs have also been found and applying the same role to those is doubtful. There is also the theory of consumption in the stage of inflammation, which could be relevant to anti-APPs. Reports about protective roles of autoantibodies are also emerging, showing lowered levels of antibodies in diseases, which could be interesting for therapeutic intervention. PMID:21718807

  10. Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells

    PubMed Central

    Wang, Yuexi; Yang, Feng; Gritsenko, Marina A.; Wang, Yingchun; Clauss, Therese; Liu, Tao; Shen, Yufeng; Monroe, Matthew E.; Lopez-Ferrer, Daniel; Reno, Theresa; Moore, Ronald J.; Klemke, Richard L.; Camp, David G.; Smith, Richard D.

    2011-01-01

    In this study, we evaluated a concatenated low pH (pH 3) and high pH (pH 10) reversed-phase liquid chromatography strategy as a first dimension for two-dimensional liquid chromatography tandem mass spectrometry (“shotgun”) proteomic analysis of trypsin-digested human MCF10A cell sample. Compared with the more traditional strong cation exchange method, the use of concatenated high pH reversed-phase liquid chromatography as a first-dimension fractionation strategy resulted in 1.8- and 1.6-fold increases in the number of peptide and protein identifications (with two or more unique peptides), respectively. In addition to broader identifications, advantages of the concatenated high pH fractionation approach include improved protein sequence coverage, simplified sample processing, and reduced sample losses. The results demonstrate that the concatenated high pH reversed-phased strategy is an attractive alternative to strong cation exchange for two-dimensional shotgun proteomic analysis. PMID:21500348

  11. Deep smarts.

    PubMed

    Leonard, Dorothy; Swap, Walter

    2004-09-01

    When a person sizes up a complex situation and rapidly comes to a decision that proves to be not just good but brilliant, you think, "That was smart." After you watch him do this a few times, you realize you're in the presence of something special. It's not raw brainpower, though that helps. It's not emotional intelligence, either, though that, too, is often involved. It's deep smarts. Deep smarts are not philosophical--they're not"wisdom" in that sense, but they're as close to wisdom as business gets. You see them in the manager who understands when and how to move into a new international market, in the executive who knows just what kind of talk to give when her organization is in crisis, in the technician who can track a product failure back to an interaction between independently produced elements. These are people whose knowledge would be hard to purchase on the open market. Their insight is based on know-how more than on know-what; it comprises a system view as well as expertise in individual areas. Because deep smarts are experienced based and often context specific, they can't be produced overnight or readily imported into an organization. It takes years for an individual to develop them--and no time at all for an organization to lose them when a valued veteran walks out the door. They can be taught, however, with the right techniques. Drawing on their forthcoming book Deep Smarts, Dorothy Leonard and Walter Swap say the best way to transfer such expertise to novices--and, on a larger scale, to make individual knowledge institutional--isn't through PowerPoint slides, a Web site of best practices, online training, project reports, or lectures. Rather, the sage needs to teach the neophyte individually how to draw wisdom from experience. Companies have to be willing to dedicate time and effort to such extensive training, but the investment more than pays for itself. PMID:15449858

  12. Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography.

    PubMed

    Deschamps, F S; Chaminade, P; Ferrier, D; Baillet, A

    2001-09-14

    Potentialities of polymerized vinyl alcohol on silica gel were assessed for class separation of simple lipids, sphingolipids, glyceroglycolipids and phospholipids by high-performance liquid chromatography. A screening of pure solvents in binary gradient elution and a chemometric approach was used to define a rugged two segment linear gradient formed from four solvents for total lipid class separation. Triethylamine and formic acid were added in all mobile phase components for acidic phospholipid separation and evaporative light scattering response enhancement. Simple analytical procedures are described for the analysis of complex lipid materials. PMID:11587330

  13. Synthesis of dynamic phase profile by the correlation technique for spatial control of optical beams in multiplexing and switching

    NASA Astrophysics Data System (ADS)

    Bugaychuk, Svitlana A.; Gnatovskyy, Vladimir O.; Sidorenko, Andrey V.; Pryadko, Igor I.; Negriyko, Anatoliy M.

    2015-11-01

    New approach for the correlation technique, which is based on multiple periodic structures to create a controllable angular spectrum, is proposed and investigated both theoretically and experimentally. The transformation of an initial laser beam occurs due to the actions of consecutive phase periodic structures, which may differ by their parameters. Then, after the Fourier transformation of a complex diffraction field, the output diffraction orders will be changed both by their intensities and by their spatial position. The controllable change of output angular spectrum is carried out by a simple control of the parameters of the periodic structures. We investigate several simple examples of such management.

  14. Marine Seismic System (MSS) deployment. Phase 5: An investigation of techniques and deployment scenarios for installation of triaxial seismometer in a borehole in the deep ocean

    NASA Astrophysics Data System (ADS)

    Wallerstedt, R. L.

    1984-03-01

    During the period 1979-1983, the Defense Advanced Research Projects Agency (DARPA) sponsored the Marine Seismic System (MSS) program that successfully accomplished two deepwater seismometer installations (MSS'81 Mid-Atlantic and MSS'83 South Pacific Sites) within specially emplaced seabed reentry boreholes. These deployments were accomplished by utilizing the Deep Sea Drilling Project's (DSDP) drillship, Glomar Challenger. Each of the three MSS operations contributed data, equipment refinement, and operational insight into the overall deep ocean deployment technology. Based upon this experience, large seismometers or other delicate instruments can be confidently deployed, utilizing the drillstring reentry technique, into seabed sediment or basalt formations in water depths to 6,096 m (20,000 ft). This report describes the design features, background analyses, and operational approach associated with the MSS Deployment System. All three MSS operations are reviewed but with particular emphasis on the latest MSS'83 South Pacific deployment and recovery activities. Important development problems and/or design uncertainties are also discussed. A list of references is provided as well as a detailed listing of all applicable reports and drawings.

  15. CLASH-VLT: The mass, velocity-anisotropy, and pseudo-phase-space density profiles of the z = 0.44 galaxy cluster MACS J1206.2-0847

    NASA Astrophysics Data System (ADS)

    Biviano, A.; Rosati, P.; Balestra, I.; Mercurio, A.; Girardi, M.; Nonino, M.; Grillo, C.; Scodeggio, M.; Lemze, D.; Kelson, D.; Umetsu, K.; Postman, M.; Zitrin, A.; Czoske, O.; Ettori, S.; Fritz, A.; Lombardi, M.; Maier, C.; Medezinski, E.; Mei, S.; Presotto, V.; Strazzullo, V.; Tozzi, P.; Ziegler, B.; Annunziatella, M.; Bartelmann, M.; Benitez, N.; Bradley, L.; Brescia, M.; Broadhurst, T.; Coe, D.; Demarco, R.; Donahue, M.; Ford, H.; Gobat, R.; Graves, G.; Koekemoer, A.; Kuchner, U.; Melchior, P.; Meneghetti, M.; Merten, J.; Moustakas, L.; Munari, E.; Regős, E.; Sartoris, B.; Seitz, S.; Zheng, W.

    2013-10-01

    Aims: We constrain the mass, velocity-anisotropy, and pseudo-phase-space density profiles of the z = 0.44 CLASH cluster MACS J1206.2-0847, using the projected phase-space distribution of cluster galaxies in combination with gravitational lensing. Methods: We use an unprecedented data-set of ≃600 redshifts for cluster members, obtained as part of a VLT/VIMOS large program, to constrain the cluster mass profile over the radial range ~0-5 Mpc (0-2.5 virial radii) using the MAMPOSSt and Caustic methods. We then add external constraints from our previous gravitational lensing analysis. We invert the Jeans equation to obtain the velocity-anisotropy profiles of cluster members. With the mass-density and velocity-anisotropy profiles we then obtain the first determination of a cluster pseudo-phase-space density profile. Results: The kinematics and lensing determinations of the cluster mass profile are in excellent agreement. This is very well fitted by a NFW model with mass M200 = (1.4 ± 0.2) × 1015 M⊙ and concentration c200 = 6 ± 1, only slightly higher than theoretical expectations. Other mass profile models also provide acceptable fits to our data, of (slightly) lower (Burkert, Hernquist, and Softened Isothermal Sphere) or comparable (Einasto) quality than NFW. The velocity anisotropy profiles of the passive and star-forming cluster members are similar, close to isotropic near the center and increasingly radial outside. Passive cluster members follow extremely well the theoretical expectations for the pseudo-phase-space density profile and the relation between the slope of the mass-density profile and the velocity anisotropy. Star-forming cluster members show marginal deviations from theoretical expectations. Conclusions: This is the most accurate determination of a cluster mass profile out to a radius of 5 Mpc, and the only determination of the velocity-anisotropy and pseudo-phase-space density profiles of both passive and star-forming galaxies for an individual

  16. Deep RNA sequencing of L. monocytogenes reveals overlapping and extensive stationary phase and sigma B-dependent transcriptomes, including multiple highly transcribed noncoding RNAs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Comprehensive, quantitative measurements of the transcriptional responses of bacterial pathogens under a variety of environmental conditions will identify specific genes and gene expression patterns important for bacterial survival, transmission and pathogenesis. The stationary phase stress respons...

  17. Circulating Cytokine Profiles and Their Relationships with Autoantibodies, Acute Phase Reactants, and Disease Activity in Patients with Rheumatoid Arthritis

    PubMed Central

    Meyer, Pieter W. A.; Hodkinson, Bridget; Ally, Mahmood; Musenge, Eustasius; Wadee, Ahmed A.; Fickl, Heidi; Tikly, Mohammed; Anderson, Ronald

    2010-01-01

    Our objective was to analyse the relationship between circulating cytokines, autoantibodies, acute phase reactants, and disease activity in DMARDs-naïve rheumatoid arthritis (RA) patients (n = 140). All cytokines were significantly higher in the RA cohort than in healthy controls. Moderate-to-strong positive intercorrelations were observed between Th1/Th2/macrophage/fibroblast-derived cytokines. RF correlated significantly with IL-1β, IL-2, IL-4, IL-10, IL-12, G-CSF, GM-CSF, IFN-γ, and TNF (P < .0001), and aCCP and aMCV with IL-1β, IL-2, IL-4, and IL-10 (P < .0002), while IL-6 correlated best with the acute phase reactants, CRP, and SAA (P < .0001). In patients with a DAS28 score of ≥5.1, IFN-γ, IL-1β, IL-1Ra, TNF, GM-CSF, and VEGF were significantly correlated (P < .04–.001) with high disease activity (HDA). Circulating cytokines in RA reflect a multifaceted increase in immune reactivity encompassing Th1 and Th2 cells, monocytes/macrophages, and synovial fibroblasts, underscored by strong correlations between these cytokines, as well as their relationships with RF, aCCP, and aMCV, with some cytokines showing promise as biomarkers of HDA. PMID:21437211

  18. Microextraction by Packed Sorbent (MEPS) and Solid-Phase Microextraction (SPME) as Sample Preparation Procedures for the Metabolomic Profiling of Urine

    PubMed Central

    Silva, Catarina; Cavaco, Carina; Perestrelo, Rosa; Pereira, Jorge; Câmara, José S.

    2014-01-01

    For a long time, sample preparation was unrecognized as a critical issue in the analytical methodology, thus limiting the performance that could be achieved. However, the improvement of microextraction techniques, particularly microextraction by packed sorbent (MEPS) and solid-phase microextraction (SPME), completely modified this scenario by introducing unprecedented control over this process. Urine is a biological fluid that is very interesting for metabolomics studies, allowing human health and disease characterization in a minimally invasive form. In this manuscript, we will critically review the most relevant and promising works in this field, highlighting how the metabolomic profiling of urine can be an extremely valuable tool for the early diagnosis of highly prevalent diseases, such as cardiovascular, oncologic and neurodegenerative ones. PMID:24958388

  19. Metabolic profiling of bile acids in human and mouse blood by LC-MS/MS in combination with phospholipid-depletion solid-phase extraction.

    PubMed

    Han, Jun; Liu, Yang; Wang, Renxue; Yang, Juncong; Ling, Victor; Borchers, Christoph H

    2015-01-20

    To obtain a more comprehensive profile of bile acids (BAs) in blood, we developed an ultrahigh performance liquid chromatography/multiple-reaction monitoring-mass spectrometry (UPLC-MRM-MS) method for the separation and detection of 50 known BAs. This method utilizes phospholipid-depletion solid-phase extraction as a new high-efficiency sample preparation procedure for BA assay. UPLC/scheduled MRM-MS with negative ion electrospray ionization enabled targeted quantitation of 43 and 44 BAs, respectively, in serum samples from seven individuals with and without fasting, as well as in plasma samples from six cholestatic gene knockout mice and six age- and gender-matched wild-type (FVB/NJ) animals. Many minor BAs were identified and quantitated in the blood for the first time. Method validation indicated good quantitation precision with intraday and interday relative standard deviations of ≤9.3% and ≤10.8%, respectively. Using a pooled human serum sample and a pooled mouse plasma sample as the two representative test samples, the quantitation accuracy was measured to be 80% to 120% for most of the BAs, using two standard-substance spiking approaches. To profile other potential BAs not included in the 50 known targets from the knockout versus wild-type mouse plasma, class-specific precursor/fragment ion transitions were used to perform UPLC-MRM-MS for untargeted detection of the structural isomers of glycine- and taurine-conjugated BAs and unconjugated tetra-hydroxy BAs. As a result, as many as 36 such compounds were detected. In summary, this UPLC-MRM-MS method has enabled the quantitation of the largest number of BAs in the blood thus far, and the results presented have revealed an unexpectedly complex BA profile in mouse plasma. PMID:25496250

  20. Characterization by solid-phase microextraction-gas chromatography of the volatile profile of protected designation of origin Montasio cheese during ripening.

    PubMed

    Innocente, N; Munari, M; Biasutti, M

    2013-01-01

    Montasio is a typical protected designation of origin (PDO) Italian semi-hard and semi-cooked cheese produced in northeast Italy from raw or thermized cow's milk. The PDO label implies that the product has distinctive characteristics that are connected to traditional production methods. The aim of this work was to precisely characterize the volatile fraction of this Italian cheese. The volatile profile can be considered a fingerprint because the flavor of a cheese variety is the result of a specific balance between the volatile compounds produced during the ripening process. Analysis of the volatile profile of Montasio cheese was performed by solid-phase microextraction-gas chromatography. Six cheesemaking trials were performed, each in a different dairy located within the Montasio cheese production area. Cheeses were analyzed at 5 stages of ripening (60, 90, 170, 300, and 365 d). Only 11 compounds were identified and measured: 5 fatty acids, 3 alcohols, 2 ketones, and 1 ester. The limited number of volatile compounds measured in the headspace of the Montasio cheese is probably due to the specific making process of this cheese, which affects evolution of the microflora and the biochemical processes of ripening. The total volatile fraction profile progressively increased from 60 to 170 d, after which time it remained almost steady. The most important contributors were found to be ethanol, short-chain fatty acids (C(2) to C(6)), diacetyl, and ethyl hexanoate. Ethanol and short-chain fatty acids increased up to 170 d, diacetyl increased up to 300 d and then declined, and ethyl hexanoate increased until the final stage. PMID:23084885

  1. The liquidus phase relations in the MgO-FeO-SiO2 ternary system in the deep lower mantle: Implication for the solidification of a basal magma ocean

    NASA Astrophysics Data System (ADS)

    Morishita, A.; Nomura, R.; Hirose, K.

    2015-12-01

    The large low shear velocity provinces (LLSVPs) observed in the lowermost mantle represent anomalously dense bodies, which may have been formed as a consequence of solidification of a basal magma ocean [Labrosse et al., 2007 Nature; Nomura et al., 2011 Nature]. Recent melting experiments have demonstrated that 1) MgSiO3-rich perovskite (bridgmanite) is the first phase to crystallize from melts with a wide range of (Mg+Fe)/Si ratios in the middle to deep lower mantle and 2) iron is preferentially partitioned into melt rather than solid [Tateno et al., 2014 JGR], suggesting that melts evolve towards an FeO-rich and SiO2-poor composition upon crystallization. However, the mineral assemblage and chemical composition of LLSVPs still remain largely uncertain. Here we carried out melting experiments at both shallow and deep lower mantle pressures using a laser-heated diamond-anvil cell (DAC), in order to examine the melting phase equilibria in the MgO-FeO-SiO2 ternary system. A focused ion beam (FIB) and field-emission-type electron microprobe (FE-EPMA) were used for textural and chemical characterization of samples recovered from the DAC. They exhibited a melting texture with quenched partial melt at the hottest part and one or two solid phases at its outside. Our result demonstrate that a crystallizing solid assemblage changes from bridgmanite, bridgmanite + (Mg,Fe)O ferropericlase, SiO2 seifertite + FeO-rich (Mg,Fe)O magnesiowüstite, and to seifertite + FeO upon fractional crystallization from a pyrolitic melt at the core-mantle boundary pressure. These also suggest that a residual melt left after extensive solidification is strongly enrich in FeO and thus exceedingly heavy and stable at the base of the mantle, which possibly represents an ultralow velocity zone (ULVZ).

  2. Non-separative headspace solid phase microextraction-mass spectrometry profile as a marker to monitor coffee roasting degree.

    PubMed

    Liberto, Erica; Ruosi, Manuela R; Cordero, Chiara; Rubiolo, Patrizia; Bicchi, Carlo; Sgorbini, Barbara

    2013-02-27

    This study describes a non-separative headspace solid phase microextraction-mass spectrometry (HS-SPME-MS) approach, in view of its application to online monitoring of a roasting process. The system can quickly provide representative and diagnostic fingerprints of the volatile fraction of samples and, in combination with appropriate chemometric pattern recognition and regression techniques, can successfully be applied to characterize, discriminate, and/or correlate patterns with the roasting process. Eighty coffee samples of different varieties, geographical origins, and blends were analyzed. The experimental HS-SPME-MS results show that the TIC fingerprint can be used to discriminate the degree of roasting; diagnostic ion abundance(s) or ratios were closely correlated with the roasting process; both could successfully be used as markers or analytical decision makers, to monitor roasting processes online, and to define quality and safety of roasted coffee. PMID:23088249

  3. Analyses of Long Non-Coding RNA and mRNA profiling using RNA sequencing during the pre-implantation phases in pig endometrium.

    PubMed

    Wang, Yueying; Xue, Songyi; Liu, Xiaoran; Liu, Huan; Hu, Tao; Qiu, Xiaotian; Zhang, Jinlong; Lei, Minggang

    2016-01-01

    Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated the expression profiles of endometrial tissue on Days 9, 12 and 15 of pregnancy and on Day 12 of non-pregnancy in Yorkshire, and performed a comprehensive analysis of long non-coding RNAs (lncRNAs) in endometrial tissue samples by using RNA sequencing. As a result, 2805 novel lncRNAs, 2,376 (301 lncRNA and 2075 mRNA) differentially expressed genes (DEGs) and 2149 novel transcripts were obtained by pairwise comparison. In agreement with previous reports, lncRNAs shared similar characteristics, such as shorter in length, lower in exon number, lower at expression level and less conserved than protein coding transcripts. Bioinformatics analysis showed that DEGs were involved in protein binding, cellular process, immune system process and enriched in focal adhesion, Jak-STAT, FoxO and MAPK signaling pathway. We also found that lncRNAs TCONS_01729386 and TCONS_01325501 may play a vital role in embryo pre-implantation. Furthermore, the expression of FGF7, NMB, COL5A3, S100A8 and PPP1R3D genes were significantly up-regulated at the time of maternal recognition of pregnancy (Day 12 of pregnancy). Our results first identified the characterization and expression profile of lncRNAs in pig endometrium during pre-implantation phases. PMID:26822553

  4. Analyses of Long Non-Coding RNA and mRNA profiling using RNA sequencing during the pre-implantation phases in pig endometrium

    PubMed Central

    Wang, Yueying; Xue, Songyi; Liu, Xiaoran; Liu, Huan; Hu, Tao; Qiu, Xiaotian; Zhang, Jinlong; Lei, Minggang

    2016-01-01

    Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated the expression profiles of endometrial tissue on Days 9, 12 and 15 of pregnancy and on Day 12 of non-pregnancy in Yorkshire, and performed a comprehensive analysis of long non-coding RNAs (lncRNAs) in endometrial tissue samples by using RNA sequencing. As a result, 2805 novel lncRNAs, 2,376 (301 lncRNA and 2075 mRNA) differentially expressed genes (DEGs) and 2149 novel transcripts were obtained by pairwise comparison. In agreement with previous reports, lncRNAs shared similar characteristics, such as shorter in length, lower in exon number, lower at expression level and less conserved than protein coding transcripts. Bioinformatics analysis showed that DEGs were involved in protein binding, cellular process, immune system process and enriched in focal adhesion, Jak-STAT, FoxO and MAPK signaling pathway. We also found that lncRNAs TCONS_01729386 and TCONS_01325501 may play a vital role in embryo pre-implantation. Furthermore, the expression of FGF7, NMB, COL5A3, S100A8 and PPP1R3D genes were significantly up-regulated at the time of maternal recognition of pregnancy (Day 12 of pregnancy). Our results first identified the characterization and expression profile of lncRNAs in pig endometrium during pre-implantation phases. PMID:26822553

  5. Reversed-Phase Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry for High-Throughput Molecular Profiling of Sea Cucumber Cerebrosides.

    PubMed

    Jia, Zicai; Cong, Peixu; Zhang, Hongwei; Song, Yu; Li, Zhaojie; Xu, Jie; Xue, Changhu

    2015-07-01

    Usually, the chemical structures of cerebrosides in sea creatures are more complicated than those from terrestrial plants and animals. Very little is known about the method for high-throughput molecular profiling of cerebrosides in sea cucumbers. In this study, cerebrosides from four species of edible sea cucumbers, specifically, Apostichopus japonicas, Thelenota ananas, Acaudina molpadioides and Bohadschia marmorata, were rapidly identified using reversed-phase liquid chromatography-quadrupole-time-of-flight mass spectrometry (RPLC-QToF-MS). [M + H](+) in positive electrospray ionization (ESI) mode were used to obtain the product ion spectra. The cerebroside molecules were selected according to the neutral loss fragments of 180 Da and then identified according to pairs of specific products of long-chain bases (LCB) and their precursor ions. A typical predominant LCB was 2-amino-1,3-dihydroxy-4-heptadecene (d17:1), which was acylated to form saturated and monounsaturated non-hydroxy and monohydroxy fatty acids with 17-25 carbon atoms. Simultaneously, the occurrence of 2-hydroxy-tricosenoic acid (C23:1h) was characteristic of sea cucumber cerebrosides, whereas this molecule was rarely discovered in plants, mammals, or fungi. The profiles of LCB and fatty acids (FA) distribution might be related to the genera of sea cucumber. These data will be useful for identification of cerebrosides using RPLC-QToF-MS. PMID:26037520

  6. Efficacy profile of a bivalent Staphylococcus aureus glycoconjugated vaccine in adults on hemodialysis: Phase III randomized study

    PubMed Central

    Fattom, Ali; Matalon, Albert; Buerkert, John; Taylor, Kimberly; Damaso, Silvia; Boutriau, Dominique

    2015-01-01

    In a previous study in end-stage renal disease (ESRD) hemodialysis patients, a single dose of Staphylococcus aureus type 5 and 8 capsular polysaccharides (T5/T8) conjugated to nontoxic recombinant Pseudomonas aeruginosa exotoxin A investigational vaccine showed no efficacy against S. aureus bacteremia 1 year post-vaccination, but a trend for efficacy was observed over the first 40 weeks post-vaccination. Vaccine efficacy (VE) of 2 vaccine doses was therefore evaluated. In a double-blind trial 3359 ESRD patients were randomized (1:1) to receive vaccine or placebo at week 0 and 35. VE in preventing S. aureus bacteremia was assessed between 3–35 weeks and 3–60 weeks post-dose-1. Anti-T5 and anti-T8 antibodies were measured. Serious adverse events (SAEs) were recorded for 42 days post-vaccination and deaths until study end. No significant difference in the incidence of S. aureus bacteremia was observed between vaccine and placebo groups between weeks 3–35 weeks post-dose 1 (VE -23%, 95%CI: -98;23, p = 0.39) or at 3–60 weeks post-dose-1 (VE -8%, 95%CI: -57;26, p = 0.70). Day 42 geometric mean antibody concentrations were 272.4 μg/ml and 242.0 μg/ml (T5 and T8, respectively) in vaccinees. SAEs were reported by 24%/25.3% of vaccinees/placebo recipients. These data do not show a protective effect of either 1 or 2 vaccine doses against S. aureus bacteremia in ESRD patients. The vaccine induced a robust immune response and had an acceptable safety profile. Further investigation suggested possible suboptimal vaccine quality (manufacturing) and a need to expand the antigen composition of the vaccine. This study is registered at www.clinicaltrials.gov NCT00071214. PMID:25483694

  7. Melting of phase D in the lower mantle and implications for recycling and storage of H2O in the deep mantle

    NASA Astrophysics Data System (ADS)

    Ghosh, Sujoy; Schmidt, Max W.

    2014-11-01

    We determined the melting phase relations and conditions of the dense hydrous magnesium silicate phase D (nominally MgSi2O4(OH)2) on composition in MgO-SiO2-H2O (MSH), MgO-Al2O3-SiO2-H2O (MASH), and FeO-MgO-Al2O3-SiO2-H2O (FMASH), and on a mixture of phase D + olivine + enstatite (MSH) at 22-32 GPa and 1000-1800 °C. Contrasting to previous studies, we performed H2O-undersaturated experiments. Bulk compositions were synthetic mixtures of brucite + silica or brucite + olivine + enstatite on the silica-rich side of the tie-line perovskite-H2O. At 22-24 GPa, the maximum thermal stability of phase D is between 1350 and 1400 °C in MSH and FMASH, but 1600 °C at 24 GPa in the Fe-free, Al-bearing bulk composition (MASH). Apparently, addition of Al2O3 increases the stability field of phase D by 200 °C, an effect that is counter balanced by addition of FeO. At 32 GPa, the stability of phase D (MSH and FMASH) is between 1350 and 1400 °C. At 22 GPa, phase D melts to a Mg-rich melt coexisting with MgSi-ilmenite + stishovite, whereas at 24-32 GPa melt coexists with perovskite and stishovite. Even melts from bulk compositions in the silica-rich part of the MSH system (molar bulk Mg/Si < 0.5) are magnesian-rich (Mg/Si molar ratio of 2-5) and are distinct from aqueous fluids and hydrous melts at lower pressures. The temperature stabilities determined in this study indicate that slabs that thermally relax when stagnating on top of the 660-km discontinuity or penetrating into the lower mantle will have their last dense hydrous magnesium silicate phase, i.e., phase D, melting and producing a magnesian and hydrous melt that will rise through the transition zone. Such a melt could be responsible for observed low velocity zones, and may be neutrally buoyant at the 410-km discontinuity and will affect the structure and dynamics of the mantle.

  8. Nanoscale phase separation in deep underdoped Bi2Sr2CuO6+δ and Ca2CuO2Cl2

    NASA Astrophysics Data System (ADS)

    Mistark, Peter; Markiewicz, Robert; Bansil, Arun

    2015-03-01

    We demonstrate that the tunneling spectra from deeply underdoped Bi2Sr2CuO6+δ (Bi2201) and Ca2CuO2Cl2 (CCOC) provide clear evidence for a nanoscale phase separation (NPS), which causes the gap to fill rather than close with doping. The phase separation extends from half-filling to a doping of x ~ 0 . 09 . Assuming that the NPS is in the form of stripes, the nodal gap, which we model as a Coulomb gap, arises from impurity pinning of the charged stripes, and ultimately drives a metal-insulator transition. This work is supported by the U.S.D.O.E.

  9. Reversed-Phase Chromatography with Multiple Fraction Concatenation Strategy for Proteome Profiling of Human MCF10A Cells

    SciTech Connect

    Wang, Yuexi; Yang, Feng; Gritsenko, Marina A.; Wang, Yingchun; Clauss, Therese RW; Liu, Tao; Shen, Yufeng; Monroe, Matthew E.; Lopez-Ferrer, Daniel; Reno, Theresa; Moore, Ronald J.; Klemke, Richard L.; Camp, David G.; Smith, Richard D.

    2011-05-01

    Two dimensional liquid chromatography (2D LC) is commonly used for shotgun proteomics to improve the analysis dynamic range. Reversed phase liquid chromatography (RPLC) has been routinely employed as the second dimensional separation prior to the mass spectrometric analysis. Construction of 2D separation with RP-RP arises a concern for the separation orthogonality. In this study, we applied a novel concatenation strategy to improve the orthogonality of 2D RP-RP formed by low pH (i.e., pH 3) and high pH (i.e., pH 10) RPLC. We confidently identified 3753 proteins (18570 unique peptides) and 5907 proteins (37633 unique peptides) from low pH RPLC-RP and high pH RPLC-RP, respectively, for a trypsin-digested human MCF10A cell sample. Compared with SCX-RP, the high pH-low pH RP-RP approach resulted in 1.8-fold and 1.6-fold in the number of peptide and protein identifications, respectively. In addition to the broader identifications, the High pH-low pH RP-RP approach has advantages including the improved protein sequence coverage, the simplified sample processing, and the reduced sample loss. These results demonstrated that the concatenation high pH-low pH RP-RP strategy is an attractive alternative to SCX for 2D LC shotgun proteomic analysis.

  10. Proteome profiling of the growth phases of Leishmania pifanoi promastigotes in axenic culture reveals differential abundance of immunostimulatory proteins.

    PubMed

    Alcolea, Pedro J; Alonso, Ana; García-Tabares, Francisco; Mena, María del Carmen; Ciordia, Sergio; Larraga, Vicente

    2016-06-01

    Leishmaniasis is a term that encompasses a compendium of neglected tropical diseases caused by dimorphic and digenetic protozoan parasites from the genus Leishmania (Kinetoplastida: Trypanosomatidae). The clinical manifestations of neotropical cutaneous leishmaniasis (NCL) caused by Leishmania pifanoi and other species of the "Leishmania mexicana complex" mainly correspond to anergic diffuse cutaneous leishmaniasis (ADCL), which is the origin of considerable morbidity. Despite the outstanding advances in the characterization of the trypanosomatid genomes and proteomes, the biology of this species has been scarcely explored. However, the close relation of L. pifanoi to the sequenced species L. mexicana and others included in the "L. mexicana complex" allowed us to perform a two-dimension electrophoresis (2DE) approach to the promastigote proteome at the differential expression level. Protein identifications were performed by matrix-assisted laser desorption-ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF). This insight has revealed similarities and differences between L. pifanoi and other species responsible for cutaneous and visceral leishmaniasis. Interestingly, certain proteins that were previously described as immunostimulatory (elongation factor 1β, trypanothione peroxidase, heat shock protein 70, enolase, GDP-forming succinyl-CoA and aldehyde dehydrogenase) are more abundant in the final growth stages of promastigotes (late-logarithmic and/or stationary phase) in the case of L. pifanoi. PMID:26992294

  11. Cytokine profile of a Holstein calf with bovine leukocyte adhesion deficiency during the acute-phase inflammatory response.

    PubMed

    Nagahata, Hajime; Hagiwara, Katsuro; Kasamatsu, Masahiko; Higuchi, Hidetoshi; Kurosawa, Takashi

    2002-12-01

    Changes in interleukin (IL)-1beta, IL-6 and IL-8 in serum, and their mRNA expression on neutrophils from a 4.6-month old Holstein young calf with bovine leukocyte adhesion deficiency (BLAD) during the acute phase were evaluated. IL-1beta concentrations in the serum of the calf with BLAD at age 143-162 days ranged from 8.7 to 16.6 ng/ml, whereas the values were less than 2.7 ng/ml in control calves. Serum IL-6 (0.04 ng/ml) was only detected on the 1st day when the animal was diagnosed with the BLAD. IL-1beta and IL-8 mRNA expression on neutrophils from the affected calf appeared to be similar to those of controls. Serum cytokine levels and their mRNA expression on neutrophils from the calf with BLAD appeared to be little affected by the deficient expression of beta(2)-integrin on leukocytes, and are considered to be modulated by the inflammatory stimuli. PMID:12520109

  12. Controlling the Al-doping profile and accompanying electrical properties of rutile-phased TiO2 thin films.

    PubMed

    Jeon, Woojin; Rha, Sang Ho; Lee, Woongkyu; Yoo, Yeon Woo; An, Cheol Hyun; Jung, Kwang Hwan; Kim, Seong Keun; Hwang, Cheol Seong

    2014-05-28

    The role of Al dopant in rutile-phased TiO2 films in the evaluation of the mechanism of leakage current reduction in Al-doped TiO2 (ATO) was studied in detail. The leakage current of the ATO film was strongly affected by the Al concentration at the interface between the ATO film and the RuO2 electrode. The conduction band offset of the interface increased with the increase in the Al dopant concentration in the rutile TiO2, which reduced the leakage current in the voltage region pertinent to the next-generation dynamic random access memory application. However, the Al doping in the anatase TiO2 did not notably increase the conduction band offset even with a higher Al concentration. The detailed analyses of the leakage conduction mechanism based on the quantum mechanical transfer-matrix method showed that Schottky emission and Fowler-Nordheim tunneling was the dominant leakage conduction mechanism in the lower and higher voltage regions, respectively. The chemical analyses using X-ray photoelectron spectroscopy corroborated the electrical test results. PMID:24749990

  13. Fe-Ni-Cu-C-S phase relations at high pressures and temperatures - The role of sulfur in carbon storage and diamond stability at mid- to deep-upper mantle

    NASA Astrophysics Data System (ADS)

    Tsuno, Kyusei; Dasgupta, Rajdeep

    2015-02-01

    Constraining the stable form of carbon in the deep mantle is important because carbon has key influence on mantle processes such as partial melting and element mobility, thereby affecting the efficiency of carbon exchange between the endogenic and exogenic reservoirs. In the reduced, mid- to deep-upper mantle, the chief host of deep carbon is expected to be graphite/diamond but in the presence of Fe-Ni alloy melt in the reduced mantle and owing to high solubility of carbon in such alloy phase, diamond may become unstable. To investigate the nature of stable, C-bearing phases in the reduced, mid- to deep-upper mantle, here we have performed experiments to examine the effect of sulfur on the phase relations of the Ni-rich portion of Fe-Ni ± Cu-C-S system, and carbon solubility in the Fe-Ni solid and Fe-Ni-S liquid alloys at 6-8 GPa and 800-1400 °C using a multianvil press. Low-temperature experiments for six starting mixes (Ni/(Fe + Ni) ∼ 0.61, 8-16 wt.% S) contain C-bearing, solid Fe-Ni alloy + Fe-Ni-C-S alloy melt + metastable graphite, and the solid alloy-out boundary is constrained, at 1150-1200 °C at 6 GPa and 900-1000 °C at 8 GPa for S-poor starting mix, and at 1000-1050 °C at 6 GPa and 900-1000 °C at 8 GPa for the S-rich starting mix. The carbon solubility in the liquid alloy significantly diminishes from 2.1 to 0.8 wt.% with sulfur in the melt increasing from 8 to 24 wt.%, irrespective of temperature. We also observed a slight decrease of carbon solubility in the liquid alloy with increasing pressure when alloy liquid contains >∼18 wt.% S, and with decreasing Ni/(Fe + Ni) ratio from 0.65 to ∼0.53. Based on our results, diamond, coexisting with Ni-rich sulfide liquid alloy is expected to be stable in the reduced, alloy-bearing oceanic mantle with C content as low as 20 to 5 ppm for mantle S varying between 100 and 200 ppm. Deep, reduced root of cratonic mantle, on the other hand, is expected to have C distributed among solid alloy, liquid alloy

  14. Patterns of GPS-TEC variation over low-latitude regions (African sector) during the deep solar minimum (2008 to 2009) and solar maximum (2012 to 2013) phases

    NASA Astrophysics Data System (ADS)

    Tariku, Yekoye Asmare

    2015-03-01

    Due to the unique geometry of the geomagnetic fields near the magnetic equator and low-latitude regions, the satellite communication system in the African sector is strongly influenced by the effects resulting from the accumulation of electrons in their ionosphere. Hence, this paper investigates the patterns of the vertical total electron content (VTEC) variation detected by the Global Positioning System (GPS) over low-latitude regions during a very low (2008 to 2009) and a high solar activity (2012 to 2013) phases. The study has been carried out by considering eight ground-based dual-frequency GPS receivers installed recently at different regions in Ethiopia. In this work, the diurnal, monthly, and seasonal variations in the GPS-VTEC have been analyzed. It has been found that the diurnal variability of VTEC has shown minimum values at around 0300 UT (0600 local time (LT)) and maximum values nearly between 1000 and 1300 UT (1300 and 1600 LT) during both the low and the high activity phases. Moreover, the maximum and minimum of monthly mean hourly VTEC values are observed in October and July, respectively, during both the low (2009) and the high solar activity (2012) phases. It has also been depicted that seasonal mean hourly VTEC values have shown maxima and minima in the March equinox and the June solstice, respectively, during both the low and the high solar activity phases.

  15. Deep RNA sequencing of L. monocytogenes reveals overlapping and extensive stationary phase and sigma B-dependent transcriptomes, including multiple highly transcribed noncoding RNAs

    PubMed Central

    2009-01-01

    Background Identification of specific genes and gene expression patterns important for bacterial survival, transmission and pathogenesis is critically needed to enable development of more effective pathogen control strategies. The stationary phase stress response transcriptome, including many σB-dependent genes, was defined for the human bacterial pathogen Listeria monocytogenes using RNA sequencing (RNA-Seq) with the Illumina Genome Analyzer. Specifically, bacterial transcriptomes were compared between stationary phase cells of L. monocytogenes 10403S and an otherwise isogenic ΔsigB mutant, which does not express the alternative σ factor σB, a major regulator of genes contributing to stress response, including stresses encountered upon entry into stationary phase. Results Overall, 83% of all L. monocytogenes genes were transcribed in stationary phase cells; 42% of currently annotated L. monocytogenes genes showed medium to high transcript levels under these conditions. A total of 96 genes had significantly higher transcript levels in 10403S than in ΔsigB, indicating σB-dependent transcription of these genes. RNA-Seq analyses indicate that a total of 67 noncoding RNA molecules (ncRNAs) are transcribed in stationary phase L. monocytogenes, including 7 previously unrecognized putative ncRNAs. Application of a dynamically trained Hidden Markov Model, in combination with RNA-Seq data, identified 65 putative σB promoters upstream of 82 of the 96 σB-dependent genes and upstream of the one σB-dependent ncRNA. The RNA-Seq data also enabled annotation of putative operons as well as visualization of 5'- and 3'-UTR regions. Conclusions The results from these studies provide powerful evidence that RNA-Seq data combined with appropriate bioinformatics tools allow quantitative characterization of prokaryotic transcriptomes, thus providing exciting new strategies for exploring transcriptional regulatory networks in bacteria. See minireivew http://jbiol.com/content/8

  16. Profiling of phytohormones and their major metabolites in rice using binary solid-phase extraction and liquid chromatography-triple quadrupole mass spectrometry.

    PubMed

    Cao, Zhao-Yun; Sun, Li-Hua; Mou, Ren-Xiang; Zhang, Lin-Ping; Lin, Xiao-Yan; Zhu, Zhi-Wei; Chen, Ming-Xue

    2016-06-17

    A high-throughput method was developed using liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) for the profiling and quantification of 43 phytohormones and their major metabolites, including auxins, abscisic acid, jasmonic acid, salicylic acid, cytokinins and gibberellins in a single sample extract. Considerable matrix effects (MEs) were observed (with most ME values in the range of 29%-84%, but maximum MEs of more than 115%, even up to 206%, existed) in sample extracts for most of the compounds studied. The application of the proposed binary solid-phase extraction using polymer anion and polymer cation exchange resins, was performed to purify 25 acidic and 18 alkaline phytohormones and their major metabolites prior to the LC-MS/MS analysis, which markedly reduced the MEs to acceptable levels, with ME values in the range of ±15%. Moreover, all of the isomers of cytokinins and their metabolites were fully separated on a sub-2μm particle C18 reverse-phase column with the optimized mobile phase consisting of methanol and 5mM ammonium formate. The method showed good linearity for all 43 analytes with regression coefficients (R(2))>0.991. Limits of detection ranged from 0.19 to 7.57 fmol for auxin, gibberellins, abscisic acid and their metabolites, 29.7 fmol for jasmonic acid, 18.1 fmol for salicylic acid, and from 0.03 to 0.31 fmol for cytokinins and their metabolites. The mean recoveries for all of the analytes were from 70.7 to 118.5%, and the inter-day precisions (n=6) were less than 18.7%, with intra-day precisions (n=6) within 25.4%. Finally, 20 compounds were successfully quantified in rice sample profiles using the proposed method, which will greatly facilitate the understanding of hormone-related regulatory networks that influence rice growth and development. To our knowledge, there are limited reports that measure this level of phytohormone species in rice samples using a single analysis. PMID:27208989

  17. Deep pockets for deep seas

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    Peter Auster, a fisheries ecologist with the National Undersea Research Center in Connecticut, plans to assess degradation of the deep-shelf seafloor from bottom trawling. Magnus Ngoile, an official with Tanzania's National Environmental Management Council, will work on building capacity of poor villagers to protect their coastline. And Alison Rieser, a lawyer with the University of Maine School of Law, will produce a textbook to educate scientists on how to apply the law for marine conservation.These individuals are among 11 recipients of the Pew Charitable Trust's 10th annual marine conservation fellowships, announced on July 12. With each recipient receiving an award of $150,000, the program is the world's largest award for marine conservationists. Other 1999 recipients will be involved with areas including investigating marine pollution in the Arctic region, examining economic incentives for conservation in Baja, Mexico, and establishing a marine conservation biology training program for minority students.

  18. Development of an achiral supercritical fluid chromatography method with ultraviolet absorbance and mass spectrometric detection for impurity profiling of drug candidates. Part I: Optimization of mobile phase composition.

    PubMed

    Lemasson, Elise; Bertin, Sophie; Hennig, Philippe; Boiteux, Hélène; Lesellier, Eric; West, Caroline

    2015-08-21

    Supercritical fluid chromatography (SFC) is a very useful tool in the purpose of impurity profiling of drug candidates, as an adequate selection of stationary phases can provide orthogonal separations so as to maximize the chances to see all impurities. The purpose of the present work is to develop a method for chemical purity assessment. The first part, presented here, focuses on mobile phase selection to ensure adequate elution and detection of drug-like molecules, while the second part focuses on stationary phase selection for optimal separation and orthogonality. The use of additives in the carbon dioxide - solvent mobile phase in SFC is now commonplace, and enables in particular to increase the number of eluted compounds and to improve peak shapes. The objective of this first part was to test different additives (acids, bases, salts and water) for their chromatographic performance assessed in gradient elution with a diode-array detector, but also for the mass responses obtained with a single-quadrupole mass detector, equipped with an electrospray ionization source (Waters ACQUITY QDa). In this project, we used a selection of one hundred and sixty compounds issued from Servier Research Laboratories to screen a set of columns and additives in SFC with a Waters ACQUITY UPC(2) system. The selected columns were all high-performance columns (1.7-1.8μm with totally porous particles or 2.6-2.7μm with superficially porous particles) with a variety of stationary phase chemistries. Initially, eight additives dissolved in the methanol co-solvent were tested on a UPC(2) ACQUITY UPC(2) HSS C18 SB column. A Derringer desirability function was used to classify the additives according to selected criteria: elution capability, peak shapes, UV baseline drift, and UV and mass responses (signal-to-noise ratios). Following these tests, the two best additives (ammonium acetate and ammonium hydroxide) were tested on a larger number of columns (10) where the two additives appeared

  19. Deep frequency modulation interferometry.

    PubMed

    Gerberding, Oliver

    2015-06-01

    Laser interferometry with pm/Hz precision and multi-fringe dynamic range at low frequencies is a core technology to measure the motion of various objects (test masses) in space and ground based experiments for gravitational wave detection and geodesy. Even though available interferometer schemes are well understood, their construction remains complex, often involving, for example, the need to build quasi-monolithic optical benches with dozens of components. In recent years techniques have been investigated that aim to reduce this complexity by combining phase modulation techniques with sophisticated digital readout algorithms. This article presents a new scheme that uses strong laser frequency modulations in combination with the deep phase modulation readout algorithm to construct simpler and easily scalable interferometers. PMID:26072834

  20. Rheological Limitations on Deep Earthquakes

    NASA Astrophysics Data System (ADS)

    Weidner, D. J.; Raterron, P.; Chen, J.; Li, L.

    2002-05-01

    The occurrence of earthquakes deeper than 50 km requires processes that differ from the friction-mitigated phenomena that control fault slip of shallow events. These earthquakes that extend to depths of about 700 km have been useful in delineating plate tectonic processes and stresses, but remain elusive as to why the stress is released as earthquakes and not dissipated by slow plastic flow. Indeed, our understanding of plate tectonic and fracture processes would not be challenged if these events did not occur at all. While the source of deviatoric stress for these earthquakes results from the dynamics of plate tectonics, the ability to store the stress and catastrophically release the stress in an earthquake is controlled by the properties of the minerals that constitute the subducting slab. Guided by our recent experimental results, we propose that the deep earthquake process is largely controlled by the rheology of the subducted material. The core of the slab for depths shallower than 400 km, at temperatures less than 500,aC, is capable of supporting high levels of shear stress with a mildly temperature dependent strength. This region is not seismogenic as it does not have access to stress instabilities. Above 600,aC, olivine becomes too weak to be seismogenic. Between 500 and 600,aC, olivine strength is highly temperature dependent and the region is ripe for runaway plastic instabilities which give rise to earthquakes. This can account for double seismic zones owing to the distorted temperature profile. If the tectonic stress is insufficient in one of these zones, then there will be only a single seismic plane. Deeper than 400 km even though temperature continues to increase, the properties of the high pressure phases of olivine, wadsleyite and ringwoodite, control the seismicity. Higher temperatures are required of these phases to access the plastic instability process, thus allowing seismicity to increase in this region. Our data for perovskite indicate that

  1. Profiling of phytohormones in rice under elevated cadmium concentration levels by magnetic solid-phase extraction coupled with liquid chromatography tandem mass spectrometry.

    PubMed

    Cai, Bao-Dong; Yin, Jia; Hao, Yan-Hong; Li, Yu-Nan; Yuan, Bi-Feng; Feng, Yu-Qi

    2015-08-01

    Phytohormones, a collection of signal small molecules with various structures, regulate a series of physiological processes of plants. For instance, they regulate the growth and development, response to biotic and abiotic stresses. Quantification of trace endogenous phytohormones is essential to elucidate their molecular mechanisms in response to stresses. However, the structural and chemical diversity of phytohormones make it difficult to purify and enrich multiple phytohormones in one-step. In the current study, a method was developed to comprehensively profile phytohormones, including 8 cytokinins (CKs), indole-3-acetic acid (IAA), abscisic acid (ABA), jasmonic acid (JA) and 10 gibberellins (GAs) by Fe3O4@TiO2-based magnetic solid-phase extraction coupled with ultra-performance liquid chromatography-electrospray tandem mass spectrometry (Fe3O4@TiO2-based MSPE-UPLC-MS/MS). In the proposed method, the phytohormones in the acetonitrile extract of plant tissues were captured and purified by one-step MSPE using Fe3O4@TiO2 as a sorbent prior to UPLC-MS/MS analysis. The sensitivity, accuracy and reproducibility of the proposed analytical method were demonstrated to satisfy the profiling of multiple phytohormones in plant tissue. We then further used the Fe3O4@TiO2-based MSPE-UPLC-MS/MS method to explore the change of phytohormones in rice under Cd stress. The results showed that CKs, IAA, ABA, JA and biological active GAs all increased under Cd stress, suggesting that these phytohormones may take part in response to Cd stress. The study may promote the further understanding of the physiological functions of phytohormones in response to Cd stress. PMID:26141271

  2. Acute Toxicity Profile and Compliance to Accelerated Radiotherapy Plus Carbogen and Nicotinamide for Clinical Stage T2-4 Laryngeal Cancer: Results of a Phase III Randomized Trial

    SciTech Connect

    Janssens, Geert O.; Terhaard, Chris H.; Doornaert, Patricia A.; Bijl, Hendrik P.; Ende, Piet van den; Chin, Alim; Pop, Lucas A.; Kaanders, Johannes H.

    2012-02-01

    Purpose: To report the acute toxicity profile and compliance from a randomized Phase III trial comparing accelerated radiotherapy (AR) with accelerated radiotherapy plus carbogen and nicotinamide (ARCON) in laryngeal cancer. Methods and Materials: From April 2001 to February 2008, 345 patients with cT2-4 squamous cell laryngeal cancer were randomized to AR (n = 174) and ARCON (n = 171). Acute toxicity was scored weekly until Week 8 and every 2-4 weeks thereafter. Compliance to carbogen and nicotinamide was reported. Results: Between both treatment arms (AR vs. ARCON) no statistically significant difference was observed for incidence of acute skin reactions (moist desquamation: 56% vs. 58%, p = 0.80), acute mucosal reactions (confluent mucositis: 79% vs. 85%, p = 0.14), and symptoms related to acute mucositis (severe pain on swallowing: 53% vs. 58%, p = 0.37; nasogastric tube feeding: 28% vs. 28%, p = 0.98; narcotic medicines required: 58% vs. 58%, p = 0.97). There was a statistically significant difference in median duration of confluent mucositis in favor of AR (2.0 vs 3.0 weeks, p = 0.01). There was full compliance with carbogen breathing and nicotinamide in 86% and 80% of the patients, with discontinuation in 6% and 12%, respectively. Adjustment of antiemesis prophylaxis was needed in 42% of patients. Conclusion: With the exception of a slight increase in median duration of acute confluent mucositis, the present data reveal a similar acute toxicity profile between both regimens and a good compliance with ARCON for clinical stage T2-4 laryngeal cancers. Treatment outcome and late morbidity will determine the real therapeutic benefit.

  3. Overloaded elution band profiles of ionizable compounds in reversed-phase liquid chromatography: Influence of the competition between the neutral and the ionic species

    SciTech Connect

    Gritti, Fabrice; Guiochon, Georges A

    2008-01-01

    The parameters that affect the shape of the band profiles of acido-basic compounds under moderately overloaded conditions (sample size less than 500 nmol for a conventional column) in RPLC are discussed. Only analytes that have a single pK{sub a} are considered. In the buffer mobile phase used for their elution, their dissociation may, under certain conditions, cause a significant pH perturbation during the passage of the band. Two consecutive injections (3.3 and 10 {micro}L) of each one of three sample solutions (0.5, 5, and 50 mM) of ten compounds were injected on five C{sub 18}-bonded packing materials, including the 5 {micro}m Xterra-C{sub 18} (121 {angstrom}), 5 {micro}m Gemini-C{sub 18} (110 {angstrom}), 5 {micro}m Luna-C{sub 18}(2) (93 {angstrom}), 3.5 {micro}m Extend-C{sub 18} (80 {angstrom}), and 2.7 {micro}m Halo-C{sub 18} (90 {angstrom}). The mobile phase was an aqueous solution of methanol buffered at a constant {sub W}{sup W}pH of 6, with a phosphate buffer. The total concentration of the phosphate groups was constant at 50 mM. The methanol concentration was adjusted to keep all the retention factors between 1 and 10. The compounds injected were phenol, caffeine, 3-phenyl 1-propanol, 2-phenyl butyric acid, amphetamine, aniline, benzylamine, p-toluidine, procainamidium chloride, and propranololium chloride. Depending on the relative values of the analyte pK{sub a} and the buffer solution pH, these analytes elute as the neutral, the cationic, or the anionic species. The influence of structural parameters such as the charge, the size, and the hydrophobicity of the analytes on the shape of its overloaded band profile is discussed. Simple but general rules predict these shapes. An original adsorption model is proposed that accounts for the unusual peak shapes observed when the analyte is partially dissociated in the buffer solution during its elution.

  4. MicroRNA Expression Profile in Bovine Granulosa Cells of Preovulatory Dominant and Subordinate Follicles during the Late Follicular Phase of the Estrous Cycle

    PubMed Central

    Gebremedhn, Samuel; Salilew-Wondim, Dessie; Ahmad, Ijaz; Sahadevan, Sudeep; Hossain, Md Munir; Hoelker, Michael; Rings, Franca; Neuhoff, Christiane; Tholen, Ernst; Looft, Christian; Schellander, Karl; Tesfaye, Dawit

    2015-01-01

    In bovine, ovarian follicles grow in a wave-like fashion with commonly 2 or 3 follicular waves emerging per estrous cycle. The dominant follicle of the follicular wave which coincides with the LH-surge becomes ovulatory, leaving the subordinate follicles to undergo atresia. These physiological processes are controlled by timely and spatially expressed genes and gene products, which in turn are regulated by post-transcriptional regulators. MicroRNAs, a class of short non-coding RNA molecules, are one of the important posttranscriptional regulators of genes associated with various cellular processes. Here we investigated the expression pattern of miRNAs in granulosa cells of bovine preovulatory dominant and subordinate follicles during the late follicular phase of bovine estrous cycle using Illumina miRNA deep sequencing. In addition to 11 putative novel miRNAs, a total of 315 and 323 known miRNAs were detected in preovulatory dominant and subordinate follicles, respectively. Moreover, in comparison with the subordinate follicles, a total of 64 miRNAs were found to be differentially expressed in preovulatory dominant follicles, of which 34 miRNAs including the miR-132 and miR-183 clusters were significantly enriched, and 30 miRNAs including the miR-17-92 cluster, bta-miR-409a and bta-miR-378 were significantly down regulated in preovulatory dominant follicles. In-silico pathway analysis revealed that canonical pathways related to oncogenesis, cell adhesion, cell proliferation, apoptosis and metabolism were significantly enriched by the predicted target genes of differentially expressed miRNAs. Furthermore, Luciferase reporter assay analysis showed that one of the differentially regulated miRNAs, the miR-183 cluster miRNAs, were validated to target the 3´-UTR of FOXO1 gene. Moreover FOXO1 was highly enriched in granulosa cells of subordinate follicles in comparison with the preovulatory dominant follicles demonstrating reciprocal expression pattern with miR-183

  5. Cross-examination of liquid-liquid extraction (LLE) and solid-phase microextraction (SPME) methods for impurity profiling of methamphetamine.

    PubMed

    Lee, Jaesin; Park, Yonghoon; Yang, Wonkyung; Chung, Heesun; Choi, Wonjun; Inoue, Hiroyuki; Kuwayama, Kenji; Park, Jeonghill

    2012-02-10

    Impurities in 48 methamphetamine (MA) samples were analyzed by liquid-liquid extraction (LLE) and headspace solid-phase microextraction (HS-SPME) methods. MPS-2 autosampler was used to improve reproducibility of SPME method, and nonadecane (C(19)) diluted with potassium bromide (KBr) powder was used as an internal standard for standardizing retention time. Impurities identified by SPME method showed different patterns compared with LLE method. Non-volatile impurities like methamphetamine dimer were not identified by SPME method, but some volatile impurities like diphenylketone, caprolactam and lots of unknowns were identified only by SPME method. 1-Phenyl-2-propanone (P2P), 1-phenyl-2-propanol and benzylcyanide peaks could be discriminated clearly by SPME method without interference of amphetamine, an artifact originates from MA degradation. Differences in the impurity patterns resulted in different clustering results. When 48 MA samples were classified into 5 LLE and 5 SPME clusters, cross-matching of the clusters resulted in 8 sub-clusters. It shows that combination of the different extraction methods can distinguish the differences which cannot be distinguished by LLE or SPME method alone, and can improve reliability of the profiling results. PMID:21376486

  6. Metabolic profiling for the identification of Huntington biomarkers by on-line solid-phase extraction capillary electrophoresis mass spectrometry combined with advanced data analysis tools.

    PubMed

    Pont, Laura; Benavente, Fernando; Jaumot, Joaquim; Tauler, Romà; Alberch, Jordi; Ginés, Silvia; Barbosa, José; Sanz-Nebot, Victoria

    2016-03-01

    In this work, an untargeted metabolomic approach based on sensitive analysis by on-line solid-phase extraction capillary electrophoresis mass spectrometry (SPE-CE-MS) in combination with multivariate data analysis is proposed as an efficient method for the identification of biomarkers of Huntington's disease (HD) progression in plasma. For this purpose, plasma samples from wild-type (wt) and HD (R6/1) mice of different ages (8, 12, and 30 weeks), were analyzed by C18 -SPE-CE-MS in order to obtain the characteristic electrophoretic profiles of low molecular mass compounds. Then, multivariate curve resolution alternating least squares (MCR-ALS) was applied to the multiple full scan MS datasets. This strategy permitted the resolution of a large number of metabolites being characterized by their electrophoretic peaks and their corresponding mass spectra. A total number of 29 compounds were relevant to discriminate between wt and HD plasma samples, as well as to follow-up the HD progression. The intracellular signaling was found to be the most affected metabolic pathway in HD mice after 12 weeks of birth, when mice already showed motor coordination deficiencies and cognitive decline. This fact agreed with the atrophy and dysfunction of specific neurons, loss of several types of receptors, and changed expression of neurotransmitters. PMID:26685060

  7. Drug-drug interaction and doping, part 1: an in vitro study on the effect of non-prohibited drugs on the phase I metabolic profile of toremifene.

    PubMed

    Mazzarino, Monica; de la Torre, Xavier; Fiacco, Ilaria; Palermo, Amelia; Botrè, Francesco

    2014-05-01

    The present study was designed to provide preliminary information on the potential impact of metabolic drug-drug interaction on the effectiveness of doping control strategies currently followed by the anti-doping laboratories to detect the intake of banned agents. In vitro assays based on the use of human liver microsomes and recombinant CYP isoforms were designed and performed to characterize the phase I metabolic profile of the prohibited agent toremifene, selected as a prototype drug of the class of selective oestrogen receptor modulators, both in the absence and in the presence of medicaments (fluconazole, ketoconazole, itraconazole, miconazole, cimetidine, ranitidine, fluoxetine, paroxetine, nefazodone) not included in the World Anti-Doping Agency list of prohibited substances and methods and frequently administered to athletes. The results show that the in vitro model developed in this study was adequate to simulate the in vivo metabolism of toremifene, confirming the results obtained in previous studies. Furthermore, our data also show that ketoconazole, itraconazole, miconazole and nefazodone cause a marked modification in the production of the metabolic products (i.e. hydroxylated and carboxylated metabolites) normally selected by the anti-doping laboratories as target analytes to detect toremifene intake; moderate variations were registered in the presence of fluconazole, paroxetine and fluoxetine; while no significant modifications were measured in the presence of ranitidine and cimetidine. This evidence imposes that the potential effect of drug-drug interactions is duly taken into account in anti-doping analysis, also for a broader significance of the analytical results. PMID:24431005

  8. Drug-drug interaction and doping, part 2: an in vitro study on the effect of non-prohibited drugs on the phase I metabolic profile of stanozolol.

    PubMed

    Mazzarino, Monica; de la Torre, Xavier; Fiacco, Ilaria; Botrè, Francesco

    2014-10-01

    The present study was designed to provide preliminary information on the potential impact of metabolic drug-drug interaction on the effectiveness of doping control strategies currently followed by the anti-doping laboratories to detect the intake of prohibited agents. In vitro assays based on the use of human liver microsomes and recombinant cytochrome P450 isoforms were developed and applied to characterize the phase I metabolic profile of the prohibited agent stanozolol, both in the absence and in the presence of substances (ketoconazole, itraconazole, miconazole, cimetidine, ranitidine, and nefazodone) not included in the World Anti-Doping Agency (WADA) list of prohibited substances and methods and frequently administered to athletes. The results show that the in vitro model utilized in this study is adequate to simulate the in vivo metabolism of stanozolol. Furthermore, our data showed that ketoconazole, itraconazole, miconazole, and nefazodone caused a marked modification in the production of the metabolic products (3'-hydroxy-stanozolol, 4β-hydroxy-stanozolol and 16β-hydroxy-stanozolol) normally selected by the anti-doping laboratories as target analytes to detect stanozolol intake. On the contrary, moderate variations were registered in the presence of cimetidine and no significant modifications were measured in the presence of ranitidine. This evidence confirms that the potential effect of drug-drug interactions is duly taken into account also in anti-doping analysis. PMID:24535830

  9. Correction of Excitation Profile in Zero Echo Time (ZTE) Imaging Using Quadratic Phase-Modulated RF Pulse Excitation and Iterative Reconstruction

    PubMed Central

    Li, Cheng; Magland, Jeremy F.; Seifert, Alan C.

    2014-01-01

    Zero-echo Time (ZTE) imaging is a promising technique for magnetic resonance imaging (MRI) of short-T2 tissue nuclei in tissues. A problem inherent to the method currently hindering its translation to the clinic is the presence of a spatial encoding gradient during excitation, which causes the hard pulse to become spatially selective, resulting in blurring and shadow artifacts in the image. While shortening radiofrequency (RF) pulse duration alleviates this problem the resulting elevated RF peak power and specific absorption rate (SAR) in practice impede such a solution. In this work, an approach is described to correct the artifacts by applying quadratic phase-modulated RF excitation and iteratively solving an inverse problem formulated from the signal model of ZTE imaging. A simple pulse sequence is also developed to measure the excitation profile of the RF pulse. Results from simulations, phantom and in vivo studies, demonstrate the effectiveness of the method in correcting image artifacts caused by inhomogeneous excitation. The proposed method may contribute toward establishing ZTE MRI as a routine 3D pulse sequence for imaging protons and other nuclei with quasi solid-state behavior on clinical scanners. PMID:24710164

  10. Photoelectric and luminescence properties of GaSb-Based nanoheterostructures with a deep Al(As)Sb/InAsSb/Al(As)Sb quantum well grown by metalorganic vapor-phase epitaxy

    SciTech Connect

    Mikhailova, M. P.; Andreev, I. A. Ivanov, E. V.; Konovalov, G. G.; Grebentshikova, E. A.; Yakovlev, Yu. P.; Hulicius, E.; Hospodkova, A.; Pangrac, Y.

    2013-08-15

    The luminescence and photoelectric properties of heterostructures with a deep Al(As)Sb/InAsSb/Al(As)Sb quantum well grown on n-GaSb substrates by metalorganic vapor-phase epitaxy are investigated. Intense superlinear luminescence and increased optical power as a function of the pump current in the photon energy range of 0.6-0.8 eV are observed at temperatures of T = 77 and 300 K. The photoelectric, current-voltage, and capacitance characteristics of these heterostructures are studied in detail. The photosensitivity is examined with photodetectors operating in the photovoltaic mode in the spectral range of 0.9-2.0 {mu}m. The sensitivity maximum at room temperature is observed at a wavelength of 1.55 {mu}m. The quantum efficiency, detectivity, and response time of the photodetectors were estimated. The quantum efficiency and detectivity at the peak of the photosensitivity spectrum are as high as {eta} = 0.6-0.7 and D{sub {lambda}max}{sup *} = (5-7) Multiplication-Sign 10{sup 10} cm Hz{sup 1/2} W{sup -1}, respectively. The photodiode response time determined as the rise time of the photoresponse pulse from 0.1 to the level 0.9 is 100-200 ps. The photodiode transmission bandwidth is 2-3 GHz. Photodetectors with a deep Al(As)Sb/InAsSb/Al(As)Sb quantum well grown on n-GaSb substrates are promising foruse in heterodyne detection systems and in information technologies.

  11. Deep Vein Thrombosis

    MedlinePlus

    MENU Return to Web version Deep Vein Thrombosis Overview What is deep vein thrombosis? Deep vein thrombosis (also called DVT) is a blood clot in a vein deep inside your body. These clots usually occur in your leg veins. While DVT is a fairly common condition, it is ...

  12. Deep Vein Thrombosis

    MedlinePlus

    Deep vein thrombosis, or DVT, is a blood clot that forms in a vein deep in the body. Most deep vein clots occur in the ... vein swells, the condition is called thrombophlebitis. A deep vein thrombosis can break loose and cause a serious problem ...

  13. Deep Vein Thrombosis

    MedlinePlus

    Deep vein thrombosis, or DVT, is a blood clot that forms in a vein deep in the body. Most deep vein clots occur in the lower leg or ... vein swells, the condition is called thrombophlebitis. A deep vein thrombosis can break loose and cause a ...

  14. Monitoring of Intense Events of Deep Water Formations in the Northwestern Mediterranean over the last five years

    NASA Astrophysics Data System (ADS)

    Houpert, Loïc; Durrieu de Madron, Xavier; Testor, Pierre; Bosse, Anthony; Mortier, Laurent

    2014-05-01

    A multi-platforms and integrated monitoring system in the framework of the Mediterranean Ocean Observing System on Environment (MOOSE) enables to monitor the deep water formation processes. Since 2007, it provides high frequency in-situ temperature, salinity vertical profiles, derived from CTD measurements on moorings, ships, and gliders, as well as horizontal and vertical currents from moorings. The aim of this study is to investigate the temporal scales associated to the deep convection phases. We also studied the interannual variability of the deep convection and its implication in the evolution of deep water thermohaline characteristics. Recent measurements from the mooring lines reveal the temporal evolution of the physical processes interfering in the phases of deep convection. Horizontal currents were strongly equivalent barotropic during each deployment and strong currents were also recorded during the different events of deep ocean convection: high frequencies vertical velocities exceeded 10 cm.s-1 during the violent vertical mixing phase and strong mesoscale horizontal currents reached 40cm.s-1 during the spreading/restratification phase. Using a eddy-detection method based on a kinematic model, more than 34 eddies crossing the mooring line were detected between November 2009 and July 2012, 19 cyclones and 15 anticyclones. The radii (resp. velocities) ranging from 1.9 km to 20.0 km (resp. 2.5 cm.s-1 to 25.1 cm.s-1 ). The main mode of the distribution of eddies radii is centered at 4km for the cyclones and 5km for the anticyclones. The apparition of newly-formed deep waters was detected in winter 2009, 2010, 2011 and 2012. In winter 2010, two newly-formed deep waters were detected after the deep convection event, both present a different potential temperature but a similar salinity, suggesting that both might be formed in the cyclonic gyre, but in different locations. In 2012, two new deep waters were detected at the mooring location, one was identified as

  15. Syneruptive deep magma transfer and shallow magma remobilization during the 2011 eruption of Shinmoe-dake, Japan—Constraints from melt inclusions and phase equilibria experiments

    NASA Astrophysics Data System (ADS)

    Suzuki, Yuki; Yasuda, Atsushi; Hokanishi, Natsumi; Kaneko, Takayuki; Nakada, Setsuya; Fujii, Toshitsugu

    2013-05-01

    model is consistent with a geophysical model that explains whole crustal deformation as being due to a single source located 7-8 km northwest of the Shinmoe-dake summit. However, even the shallowest estimated source of this deformation (7.5-6.2 km) is deeper than the SA reservoir, which thus requires a contribution of deeper BA magmas to the observed deformation. Remobilization of mush-like SA magma occurred in two stages before the early sub-Plinian event. Firstly, precursor mixing with BA magma and associated heating occurred (925-871 °C; stage-1 of ≥ 350 h), followed by final mixing with BA magma (stage-2). MgO profiles of magnetite phenocrysts define timescales of 0.7-15.2 h from this final mixing to eruption. The mixed and heated magmas, and stagnant mush that existed in the SA reservoir in the precursor stage, were finally erupted together. Magnetite phenocrysts in the Feb 18 ash reveal the occurrence of continuous erosion of the stagnant mush during the course of the 2011 eruptive activity.

  16. Comprehensive profiling of ribonucleosides modification by affinity zirconium oxide-silica composite monolithic column online solid-phase microextraction - Mass spectrometry analysis.

    PubMed

    Jiang, Han-Peng; Chu, Jie-Mei; Lan, Meng-Dan; Liu, Ping; Yang, Na; Zheng, Fang; Yuan, Bi-Feng; Feng, Yu-Qi

    2016-09-01

    More than 140 modified ribonucleosides have been identified in RNA. Determination of endogenous modified ribonucleosides in biological fluids may serve as non-invasive disease diagnostic strategy. However, detection of the modified ribonucleosides in biological fluids is challenging, especially for the low abundant modified ribonucleosides due to the serious matrix interferences of biological fluids. Here, we developed a facile preparation strategy and successfully synthesized zirconium oxide-silica (ZrO2/SiO2) composite capillary monolithic column that exhibited excellent performance for the selective enrichment of cis-diol-containing compounds. Compared with the boronate-based affinity monolith, the ZrO2/SiO2 monolith showed ∼2 orders of magnitude higher extraction capacity and can be used under physiological pH (pH 6.5-7.5). Using the prepared ZrO2/SiO2 composite monolith as the trapping column and reversed-phase C18 column as the analytical column, we further established an online solid-phase microextraction (SPME) in combination with liquid chromatography-mass spectrometry (online SPME-LC-MS/MS) analysis for the comprehensive profiling of ribonucleosides modification in human urine. Our results showed that 68 cis-diol-containing ribosylated compounds were identified in human urine, which is, to the best of our knowledge, the highest numbers of cis-diol-containing compounds were determined in a single analysis. It is worth noting that four modified ribonucleosides were discovered in the human urine for the first time. In addition, the quantification results from the pooled urine samples showed that compared to healthy controls, the contents of sixteen ribose conjugates in the urine of gastric cancer, eleven in esophagus cancer and seven in lymphoma increased more than two folds. Among these ribose conjugates, four ribose conjugates increased more than two folds in both gastric cancer and esophagus cancer; three ribose conjugates increased more than two

  17. A powerful methodological approach combining headspace solid phase microextraction, mass spectrometry and multivariate analysis for profiling the volatile metabolomic pattern of beer starting raw materials.

    PubMed

    Gonçalves, João L; Figueira, José A; Rodrigues, Fátima P; Ornelas, Laura P; Branco, Ricardo N; Silva, Catarina L; Câmara, José S

    2014-10-01

    The volatile metabolomic patterns from different raw materials commonly used in beer production, namely barley, corn and hop-derived products - such as hop pellets, hop essential oil from Saaz variety and tetra-hydro isomerized hop extract (tetra hop), were established using a suitable analytical procedure based on dynamic headspace solid-phase microextraction (HS-SPME) followed by thermal desorption gas chromatography-quadrupole mass spectrometry detection (GC-qMS). Some SPME extraction parameters were optimized. The best results, in terms of maximum signal recorded and number of isolated metabolites, were obtained with a 50/30 μm DVB/CAR/PDMS coating fiber at 40 °C for 30 min. A set of 152 volatile metabolites comprising ketones (27), sesquiterpenes (26), monoterpenes (19), aliphatic esters (19), higher alcohols (15), aldehydes (11), furan compounds (11), aliphatic fatty acids (9), aliphatic hydrocarbons (8), sulphur compounds (5) and nitrogen compounds (2) were positively identified. Each raw material showed a specific volatile metabolomic profile. Monoterpenes in hop essential oil and corn, sesquiterpenes in hop pellets, ketones in tetra hop and aldehydes and sulphur compounds in barley were the predominant chemical families in the targeted beer raw materials. β-Myrcene was the most dominant volatile metabolite in hop essential oil, hop pellets and corn samples while, in barley, the predominant volatile metabolites were dimethyl sulphide and 3-methylbutanal and, in tetra hop, 6-methyl-2-pentanone and 4-methyl-2-pentanone. Principal component analysis (PCA) showed natural sample grouping among beer raw materials. PMID:24799238

  18. Detection of deep water formation from remote sensing chlorophyll in the NW Mediterranean Sea

    NASA Astrophysics Data System (ADS)

    Bernardello, Raffaele; Bahamon, Nixon; Ahumada, Miguel-Angel; Martin, Adrian; Henson, Stephanie

    2015-04-01

    The Northwestern Mediterranean Sea is one of the few regions in the world where Deep Water Formation (DWF) occurs. During wintertime cold and dry winds that typically occur in strong bursts lasting a few days, are able to erode the near-surface stability over this area, exposing the weakly stratified underwaters and initiate a phase of violent mixing and deep convection. DWF is not a steady-state process that recurs every year. Variations in wind stress and heat flux over the winter can induce a marked interannual variability: during some years the process is specially intense and completely absent during others. The extent of the area over which DWF occurs is also uncertain. The interannual variability of the DWF process is also associated to the variability in the seasonal phytoplankton dynamics over the area. The extent of the vertical mixing set the total amount of nutrients available for the phytoplankton during the following spring bloom. However, before the bloom, when deep convection is still active, surface chlorophyll (an index for phytoplankton biomass) is vertically diluted showing low surface concentration. The occurrence of these patches of anomalously low chlorophyll concentration can, in principle, be associated to the presence of active deep convection. In this study we investigate the possibility of exploiting such association in order to quantify the duration of deep convection and the extent of the area over which it occurs. These goals will be achieved through the analysis of remote sensing chlorophyll data and in-situ Argo-floats profiles.

  19. Deep learning for computational biology.

    PubMed

    Angermueller, Christof; Pärnamaa, Tanel; Parts, Leopold; Stegle, Oliver

    2016-01-01

    Technological advances in genomics and imaging have led to an explosion of molecular and cellular profiling data from large numbers of samples. This rapid increase in biological data dimension and acquisition rate is challenging conventional analysis strategies. Modern machine learning methods, such as deep learning, promise to leverage very large data sets for finding hidden structure within them, and for making accurate predictions. In this review, we discuss applications of this new breed of analysis approaches in regulatory genomics and cellular imaging. We provide background of what deep learning is, and the settings in which it can be successfully applied to derive biological insights. In addition to presenting specific applications and providing tips for practical use, we also highlight possible pitfalls and limitations to guide computational biologists when and how to make the most use of this new technology. PMID:27474269

  20. Taoism and Deep Ecology.

    ERIC Educational Resources Information Center

    Sylvan, Richard; Bennett, David

    1988-01-01

    Contrasted are the philosophies of Deep Ecology and ancient Chinese. Discusses the cosmology, morality, lifestyle, views of power, politics, and environmental philosophies of each. Concludes that Deep Ecology could gain much from Taoism. (CW)

  1. Deep Ecology and Subjectivity.

    ERIC Educational Resources Information Center

    Foley, Grover

    1988-01-01

    Describes Deep Ecology and criticizes its limitations. Discusses mysticism, the bomb, freedom, subjectivity and power as they are addressed by Deep Ecology. Stresses the need to teach ecological balance. (CW)

  2. Deep vein thrombosis - discharge

    MedlinePlus

    You were treated for deep venous thrombosis (DVT). This is a condition in which a blood clot forms in a vein that is not on ... especially if it gets worse upon taking a deep breath in You cough up blood

  3. Deep venous thrombosis

    MedlinePlus

    Deep venous thrombosis is a condition that occurs when a blood clot forms in a vein deep inside a part ... M, et al. Executive Summary: Antithrombotic Therapy and Prevention of Thrombosis. 9th ed. American College of Chest ...

  4. Preventing Deep Vein Thrombosis

    MedlinePlus

    ... Patient Education FAQs Preventing Deep Vein Thrombosis Patient Education Pamphlets - Spanish Preventing Deep Vein Thrombosis FAQ174, August 2011 PDF ... Your Practice Patient Safety & Quality Payment Reform (MACRA) Education & Events Annual ... Pamphlets Teen Health About ACOG About Us Leadership & ...

  5. Deep atomic force microscopy

    SciTech Connect

    Barnard, H.; Drake, B.; Randall, C.; Hansma, P. K.

    2013-12-15

    The Atomic Force Microscope (AFM) possesses several desirable imaging features including the ability to produce height profiles as well as two-dimensional images, in fluid or air, at high resolution. AFM has been used to study a vast selection of samples on the scale of angstroms to micrometers. However, current AFMs cannot access samples with vertical topography of the order of 100 μm or greater. Research efforts have produced AFM scanners capable of vertical motion greater than 100 μm, but commercially available probe tip lengths are still typically less than 10 μm high. Even the longest probe tips are below 100 μm and even at this range are problematic. In this paper, we present a method to hand-fabricate “Deep AFM” probes with tips of the order of 100 μm and longer so that AFM can be used to image samples with large scale vertical topography, such as fractured bone samples.

  6. Deep Vein Thrombosis

    MedlinePlus

    ... page from the NHLBI on Twitter. What Is Deep Vein Thrombosis? Español Deep vein thrombosis (throm-BO-sis), or DVT, is a blood clot that forms in a vein deep in the body. Blood clots occur when blood ...

  7. Deep Web video

    SciTech Connect

    None Available

    2009-06-01

    To make the web work better for science, OSTI has developed state-of-the-art technologies and services including a deep web search capability. The deep web includes content in searchable databases available to web users but not accessible by popular search engines, such as Google. This video provides an introduction to the deep web search engine.

  8. Deep Web video

    ScienceCinema

    None Available

    2012-03-28

    To make the web work better for science, OSTI has developed state-of-the-art technologies and services including a deep web search capability. The deep web includes content in searchable databases available to web users but not accessible by popular search engines, such as Google. This video provides an introduction to the deep web search engine.

  9. Deep Space Telecommunications

    NASA Technical Reports Server (NTRS)

    Kuiper, T. B. H.; Resch, G. M.

    2000-01-01

    The increasing load on NASA's deep Space Network, the new capabilities for deep space missions inherent in a next-generation radio telescope, and the potential of new telescope technology for reducing construction and operation costs suggest a natural marriage between radio astronomy and deep space telecommunications in developing advanced radio telescope concepts.

  10. Toxicity Profile and Pharmacokinetic Study of A Phase I Low-Dose Schedule-Dependent Radiosensitizing Paclitaxel Chemoradiation Regimen for Inoperable Non-Small-Cell Lung Cancer

    SciTech Connect

    Chen, Yuhchyau Pandya, Kishan J.; Feins, Richard; Johnstone, David W.; Watson, Thomas; Smudzin, Therese; Keng, Peter C.

    2008-06-01

    Purpose: We report the toxicity profile and pharmacokinetic data of a schedule-dependent chemoradiation regimen using pulsed low-dose paclitaxel for radiosensitization in a Phase I study for inoperable non-small-cell lung cancer. Methods and Materials: Paclitaxel at escalating doses of 15 mg/m{sup 2}, 20 mg/m{sup 2}, and 25 mg/m{sup 2} were infused on Monday, Wednesday, and Friday with daily chest radiation in cohorts of 6 patients. Daily radiation was delayed for maximal G2/M arrest and apoptotic effect, an observation from preclinical investigations. Plasma paclitaxel concentration was determined by high-performance liquid chromatography. Results: Dose-limiting toxicities included 3 of 18 patients with Grade 3 pneumonitis and 3 of 18 patients with Grade 3 esophagitis. There was no Grade 4 or 5 pneumonitis or esophagitis. There was also no Grade 3 or 4 neutropenia, thrombocytopenia, anemia or neuropathy. For Dose Levels I (15 mg/m{sup 2}), II (20 mg/m{sup 2}), and III (25 mg/m{sup 2}), the mean peak plasma level was 0.23 {+-} 0.06 {mu}mol/l, 0.32 {+-} 0.05 {mu}mol/l, and 0.52 {+-} 0.14 {mu}mol/l, respectively; AUC was 0.44 {+-} 0.09 {mu}mol/l, 0.61 {+-} 0.1 {mu}mol/l, and 0.96 {+-} 0.23 {mu}mol/l, respectively; and duration of drug concentration >0.05 {mu}mol/l (t > 0.05 {mu}mol/l) was 1.6 {+-} 0.3 h, 1.9 {+-} 0.2 h, and 3.0 {+-} 0.9 h, respectively. Conclusion: Pulsed low-dose paclitaxel chemoradiation is associated with low toxicity. Pharmacokinetic data showed that plasma paclitaxel concentration >0.05 {mu}mol/l for a minimum of 1.6 h was sufficient for effective radiosensitization.

  11. Oceanic crust deep seismic survey

    NASA Astrophysics Data System (ADS)

    McBride, J. H.; White, R. S.

    In September 1991, the British Institutions Reflection Profiling Syndicate (BIRPS) collected 578 km of deep seismic reflection profiles over the oceanic crust beneath the Cape Verde abssyal plain in approximately 4900 m of water (Fig. 1). The survey, under the direction of J. H. McBride, was undertaken in response to a proposal made by R. S. White at the 1990 BIRPS open syndicate meeting in Birmingham, England, and was acquired using GECO-PRAKLA'S M/V Bin Hai 511. The survey consisted of two strike lines parallel to magnetic sea-floor lineations and nine orthogonal crossing lines oriented parallel to the spreading direction (Fig. 2). Adjacent lines are spaced at 4 km. For the first time, this provides the ability to map oceanic crust in “3D,” since the line spacing is less than or equal to the Fresnel-zone diameter for the lower crust.

  12. Modeling ocean deep convection

    NASA Astrophysics Data System (ADS)

    Canuto, V. M.; Howard, A.; Hogan, P.; Cheng, Y.; Dubovikov, M. S.; Montenegro, L. M.

    The goal of this study is to assess models for Deep Convection with special emphasis on their use in coarse resolution ocean general circulation models. A model for deep convection must contain both vertical transport and lateral advection by mesoscale eddies generated by baroclinic instabilities. The first process operates mostly in the initial phases while the second dominates the final stages. Here, the emphasis is on models for vertical mixing. When mesoscales are not resolved, they are treated with the Gent and McWilliams parameterization. The model results are tested against the measurements of Lavender, Davis and Owens, 2002 (LDO) in the Labrador Sea. Specifically, we shall inquire whether the models are able to reproduce the region of " deepest convection," which we shall refer to as DC (mixed layer depths 800-1300 m). The region where it was measured by Lavender et al. (2002) will be referred to as the LDO region. The main results of this study can be summarized as follows. 3° × 3° resolution. A GFDL-type OGCM with the GISS vertical mixing model predicts DC in the LDO region where the vertical heat diffusivity is found to be 10 m 2 s -1, a value that is quite close to the one suggested by heuristic studies. No parameter was changed from the original GISS model. However, the GISS model also predicts some DC in a region to the east of the LDO region. 3° × 3° resolution. A GFDL-type OGCM with the KPP model (everything else being the same) does not predict DC in the LDO region where the vertical heat diffusivity is found to be 0.5 × 10 -4 m 2 s -1 which is the background value. The KPP model yields DC only to the east of the LDO region. 1° × 1° resolution. In this case, a MY2.5 mixing scheme predicts DC in the LDO region. However, it also predicts DC to the west, north and south of it, where it is not observed. The behavior of the KPP and MY models are somewhat anti-symmetric. The MY models yield too low a mixing in stably stratified flows since they

  13. Basin-Wavelength Equatorial Deep Jet Signals Across Three Oceans

    NASA Astrophysics Data System (ADS)

    Youngs, M. K.; Johnson, G. C.

    2015-12-01

    Equatorial Deep Jets (EDJs) are equatorially trapped, stacked, zonal currents that reverse direction every few hundred meters in depth throughout much of the water column. This study evaluates their structure observationally in all three oceans using new high vertical resolution Argo float conductivity-temperature-depth (CTD) instrument profiles from 2010--2014 augmented with historical shipboard CTD from 1972--2014 and lower vertical resolution Argo float profiles from 2007--2014. Vertical strain of density is calculated from the profiles and analyzed in a stretched vertical coordinate system determined from the mean vertical density structure. The power spectra of vertical strain in each basin are analyzed using a wavelet decomposition. In the Indian and Pacific oceans, there are two distinct peaks in the power spectra, one Kelvin-wave-like and the other entirely consistent with the dispersion relation of a linear first-meridional-mode equatorial Rossby wave. In the Atlantic Ocean, the first-meridional-mode Rossby wave signature is very strong, and dominates. In all three ocean basins Rossby-wave-like signatures are coherent across the basin width, and appear to have wavelengths the scale of the basin width, with periods of about 5 years in the Indian and Atlantic oceans and about 12 years in the Pacific Ocean. Their observed meridional scales are about 1.5 times the linear theoretical values. Their phase propagation is downward with time, implying upward energy propagation if linear wave dynamics hold.

  14. Cassini-VIMS observations of Saturn's main rings: I. Spectral properties and temperature radial profiles variability with phase angle and elevation

    NASA Astrophysics Data System (ADS)

    Filacchione, G.; Ciarniello, M.; Capaccioni, F.; Clark, R. N.; Nicholson, P. D.; Hedman, M. M.; Cuzzi, J. N.; Cruikshank, D. P.; Dalle Ore, C. M.; Brown, R. H.; Cerroni, P.; Altobelli, N.; Spilker, L. J.

    2014-10-01

    The spectral properties and thermal behavior of Saturn's rings are determined from a dataset of ten radial mosaics acquired by Cassini-VIMS (Visual and Infrared Mapping Spectrometer) between October 29th 2004 and January 27th 2010 with phase angle ranging between 5.7° and 132.4° and elevation angles between -23.5° and 2.6°. These observations, after reduction to spectrograms, e.g. 2D arrays containing the VIS-IR (0.35-5.1 μm) spectral information versus radial distance from Saturn (from 73.500 to 141.375 km, 400 km/bin), allow us to compare the derived spectral and thermal properties of the ring particles on a common reference. Spectral properties: rings spectra are characterized by an intense reddening at visible wavelengths while they maintain a strong similarity with water ice in the infrared domain. Significant changes in VIS reddening, water ice abundance and grain sizes are observed across different radial regions resulting in correlation with optical depth and local structures. The availability of observations taken at very different phase angles allows us to examine spectrophotometric properties of the ring's particles. When observed at high phase angles, a remarkable increase of visible reddening and water ice band depths is found, probably as a consequence of the presence of a red-colored contaminant intimately mixed within water ice grains and of multiple scattering. At low phases the analysis of the 3.2-3.6 μm range shows faint spectral signatures at 3.42-3.52 μm which are compatible with the CH2 aliphatic stretch. The 3.29 μm PAH aromatic stretch absorption is not clearly detectable on this dataset. VIMS results indicate that ring particles contain about 90-95% water ice while the remaining 5-10% is consistent with different contaminants like amorphous carbon or tholins. However, we cannot exclude the presence of nanophase iron or hematite produced by iron oxidation in the rings tenuous oxygen atmosphere, intimately mixed with the ice grains

  15. Modeling ocean deep convection

    NASA Astrophysics Data System (ADS)

    Canuto, V. M.; Howard, A.; Hogan, P.; Cheng, Y.; Dubovikov, M. S.; Montenegro, L. M.

    The goal of this study is to assess models for Deep Convection with special emphasis on their use in coarse resolution ocean general circulation models. A model for deep convection must contain both vertical transport and lateral advection by mesoscale eddies generated by baroclinic instabilities. The first process operates mostly in the initial phases while the second dominates the final stages. Here, the emphasis is on models for vertical mixing. When mesoscales are not resolved, they are treated with the Gent and McWilliams parameterization. The model results are tested against the measurements of Lavender, Davis and Owens, 2002 (LDO) in the Labrador Sea. Specifically, we shall inquire whether the models are able to reproduce the region of " deepest convection," which we shall refer to as DC (mixed layer depths 800-1300 m). The region where it was measured by Lavender et al. (2002) will be referred to as the LDO region. The main results of this study can be summarized as follows. 3° × 3° resolution. A GFDL-type OGCM with the GISS vertical mixing model predicts DC in the LDO region where the vertical heat diffusivity is found to be 10 m 2 s -1, a value that is quite close to the one suggested by heuristic studies. No parameter was changed from the original GISS model. However, the GISS model also predicts some DC in a region to the east of the LDO region. 3° × 3° resolution. A GFDL-type OGCM with the KPP model (everything else being the same) does not predict DC in the LDO region where the vertical heat diffusivity is found to be 0.5 × 10 -4 m 2 s -1 which is the background value. The KPP model yields DC only to the east of the LDO region. 1° × 1° resolution. In this case, a MY2.5 mixing scheme predicts DC in the LDO region. However, it also predicts DC to the west, north and south of it, where it is not observed. The behavior of the KPP and MY models are somewhat anti-symmetric. The MY models yield too low a mixing in stably stratified flows since they

  16. Influence of ocean surface conditions on atmospheric vertical thermodynamic structure and deep convection

    NASA Technical Reports Server (NTRS)

    Fu, Rong; Del Genio, Anthony D.; Rossow, William B.

    1994-01-01

    The authors analyze the influence of Sea Surface Temperature (SST) and surface wind divergence on atmospheric thermodynamic structure and the resulting effects on the occurrence of deep convection using National Meteorological Center radiosonde data and International Satellite Cloud Climatology Program data for July 1983-July 1985. The onset of deep convection requires not only the existence of positive convective available potential energy (CAPE), but also an unstable planetary boundary layer (PBL). A stable PBL is observed to suppress deep convection even when CAPE is positive. Variations of SST have a major effect on CAPE, but surface wind divergence can also affect deep convection by changing the lapse rate in the lower troposphere and humidity in the PBL. Specifically, when SST is greater than or equal to 28 C, CAPE is always positive, and surface wind divergence does not qualitatively change the buoyancy profile above the PBL. Strong surface wind divergence, however, stabilizes the PBL so as to suppress the initiation of deep convection. In warm SST regions, CAPE is greater than 0 regardless of assumptions about condensate loading, although the pseudoadiabatic limit is more consistent with the observed deep convection than the reversible moist-adiabatic limit under these circumstances. When SST is less than 27 C, CAPE is usually negative and inhibits convection, but strong surface wind convergence can destabilize the inversion layer and moisten the PBL enough to make the atmosphere neutrally stable in the mean. As a result, deep convection is generally enhanced either when SST is greater than or equal to 28 C in the absence of strong surface wind divergence or when strong surface wind convergence occurs even if SST is less than 27 C. The anomalous suppression of deep convection in the warm area of the equatorial west Pacific lying between the intertropical convergence zone (ITCZ) and south Pacific convergence zone (SPCZ) is probably caused by dryness in the

  17. Evaluation of mobile phase characteristics on three zwitterionic columns in hydrophilic interaction liquid chromatography mode for liquid chromatography-high resolution mass spectrometry based untargeted metabolite profiling of Leishmania parasites.

    PubMed

    Zhang, Rong; Watson, David G; Wang, Lijie; Westrop, Gareth D; Coombs, Graham H; Zhang, Tong

    2014-10-01

    It has been reported that HILIC column chemistry has a great effect on the number of detected metabolites in LC-HRMS-based untargeted metabolite profiling studies. However, no systematic investigation has been carried out with regard to the optimisation of mobile phase characteristics. In this study using 223 metabolite standards, we explored the retention mechanisms on three zwitterionic columns with varied mobile phase composition, demonstrated the interference from poor chromatographic peak shapes on the output of data extraction, and assessed the quality of chromatographic signals and the separation of isomers under each LC condition. As expected, on the ZIC-cHILIC column the acidic metabolites showed improved chromatographic performance at low pH which can be attributed to the opposite arrangement of the permanently charged groups on this column in comparison with the ZIC-HILIC column. Using extracts from the protozoan parasite Leishmania, we compared the numbers of repeatedly detected LC-HRMS features under different LC conditions with putative identification of metabolites not amongst the standards being based on accurate mass (±3ppm). Besides column chemistry, the pH of the mobile phase plays a key role in not only determining the retention mechanisms of solutes but also the output of the LC-HRMS data processing. Fast evaporation of ammonium carbonate produced less ion suppression in ESI source and consequently improved the detectability of the metabolites in low abundance in comparison with other ammonium salts. Our results show that the combination of a ZIC-pHILIC column with an ammonium carbonate mobile phase, pH 9.2, at 20mM in the aqueous phase or 10mM in both aqueous and organic mobile phase components, provided the most suitable LC conditions for LC-HRMS-based untargeted metabolite profiling of Leishmania parasite extracts. The signal reliability of the mass spectrometer used in this study (Exactive Orbitrap) was also investigated. PMID:25160959

  18. Experimental Profiling of a Non-truncated Focused Gaussian Beam and Fine-tuning of the Quadratic Phase in the Fresnel Gaussian Shape Invariant

    SciTech Connect

    S., Juan Manuel Franco; Cywiak, Moises; Cywiak, David; Mourad, Idir

    2015-06-24

    A homodyne profiler is used for recording the intensity distribution of focused non-truncated Gaussian beams. The spatial distributions are obtained at planes in the vicinity of the back-focal plane of a focusing lens placed at different distances from a He–Ne laser beam with a Gaussian intensity profile. Comparisons of the experimental data with those obtained from the analytical equations for an ideal focusing lens allow us to propose formulae to fine-tune the quadratic term in the Fresnel Gaussian shape invariant at each interface of the propagated field. Furthermore, we give analytical expressions to calculate adequately the propagation of the field through an optical system.

  19. Thorium-230 profiling in deep-sea sediments: High-resolution records of flux and dissolution of carbonate in the equatorial Atlantic during the last 24,000 years

    NASA Astrophysics Data System (ADS)

    Francois, Roger; Bacon, Michael P.; Suman, Daniel O.

    1990-10-01

    Variations in carbonate flux and dissolution, which occurred in the equatorial Atlantic during the last 24,000 years, have been estimated by a new approach that allows the point-by-point determination of paleofluxes to the seafloor. An unprecedented time resolution can thus be obtained which allows sequencing of the relatively rapid events occurring during deglaciation. The method is based on observations that the flux of unsupported 230Th into deep-sea sediments is nearly independent of the total mass flux and is close to the production rate. Thus excess 230Th activity in sediments can be used as a reference against which fluxes of other sedimentary components can be estimated. The study was conducted at two sites (Ceará Rise; western equatorial Atlantic, and Sierra Leone Rise; eastern equatorial Atlantic) in cores raised from three different depths at each site. From measurements of 230Th and CaCO3, changes in carbonate flux with time and depth were obtained. A rapid increase in carbonate production, starting at the onset of deglaciation, was found in both areas. This event may have important implications for the postglacial increase in atmospheric CO2 by increasing the global carbonate carbon to organic carbon rain ratio and decreasing the alkalinity of surface waters (and possibly the North Atlantic Deep Water). Increased carbonate dissolution occurred in the two regions during deglaciation, followed by a minimum during mid-Holocene and renewed intensification of dissolution in late Holocene. During the last 16,000 years, carbonate dissolution was consistently more pronounced in the western than in the eastern basin, reflecting the influence of Antarctic Bottom Water in the west. This trend was reversed during stage 2, possibly due to the accumulation of metabolic CO2 below the level of the Romanche Fracture Zone in the eastern basin.

  20. Progress in deep lithospheric exploration of the continental China: A review of the SinoProbe

    NASA Astrophysics Data System (ADS)

    Dong, Shu-Wen; Li, Ting-Dong; Lü, Qing-Tian; Gao, Rui; Yang, Jing-Sui; Chen, Xuan-Hua; Wei, Wen-Bo; Zhou, Qi

    2013-10-01

    The SinoProbe, deep exploration in China, is a multidisciplinary earth science research program that aims at revealing the composition, structure and evolution of the continental lithosphere in China. The SinoProbe (2008-2012) has successfully conducted research and field experiments on determining the crustal and mantle structures using new deep seismic and magnetotelluric (MT) exploration. This has allowed the accumulation of new exciting data that have significantly accelerated China's development on deep lithospheric exploration. The new data also led to new understandings on the Mesozoic and Cenozoic geological evolution of the continental China. The main results of the SinoProbe so far include (1) a collection of ca.6000 km long seismic reflection profile data, (2) a nation-wide geochemical baseline, (3) a nation-wide 4° × 4° MT array and regional 1° × 1° MT arrays in the North China and the Qinghai-Tibet Plateau, (4) three dimensional (3-D) exploration of ore districts in the eastern China, (5) several continental scientific drilling holes, (6) regional in-situ stress monitoring networks, (7) geodynamic modeling of the lithosphere underneath the continental China, and (8) instrumentation development for deep exploration in China, etc. For the first time, the SinoProbe has obtained deep seismic reflection evidence for the Moho surface below the thick crust of the central Qinghai-Tibet Plateau. It also reveals dipping fabrics in the lithospheric mantle beneath the northeastern China. The preliminary results from MT array observation of the SinoProbe show an abnormal electric-conductivity structure of the lithosphere beneath the Ordos basin, providing important evidence for the evolution mechanism of the North China craton (NCC). Generally, the SinoProbe has conducted successfully during its initial phase (2008-2012), which has settled a foundation for the next phase of the SinoProbe in the future.

  1. The deep space network

    NASA Technical Reports Server (NTRS)

    1977-01-01

    Presented is Deep Space Network (DSN) progress in flight project support, tracking and data acquisition (TDA) research and technology, network engineering, hardware and software implementation, and operations.

  2. The deep space network

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Summaries are given of Deep Space Network progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implementation, and operations.

  3. Deep space antennas

    NASA Astrophysics Data System (ADS)

    Bell, Peter M.

    Three 26-m tracking antennas operated by the NASA Deep Space Network at Goldstone, Calif.; Madrid, Spain; and near Canberra, Australia, will cease operations on Dec. 1, 1981. The stations will continue to operate 64-m and 34-m deep space tracking antennas. Ending operation of the 26-m antennas will cause a reduction of about 30%; of the Deep Space Network tracking and data acquisition capability. This means less support for NASA planetary spacecraft. Currently, the Deep Space Network is supporting Voyagers 1 and 2, Helios 1, the Mars Viking 1 Lander and Pioneers 6 through 12.

  4. Computer simulation of deep sulfate reduction in sediments of the Amazon Fan

    NASA Astrophysics Data System (ADS)

    Adler, M.; Hensen, C.; Kasten, S.; Schulz, H. D.

    Pore water concentration profiles of sediments at a site on the Amazon Fan were investigated and simulated with the numerical model CoTReM (column transport and reaction model) to reveal the biogeochemical processes involved. The pore water profiles for gravity core GeoB 4417-7 showed a distinct sulfate-methane transition zone in which deep sulfate reduction occurs. Only a small sulfide peak could be observed at the reaction zone. Due to high amounts of iron minerals, the produced sulfide is instantaneously precipitated in form of iron sulfides. We present a simulation which starts from a steady state system with respect to pore water profiles for methane and sulfate. Furthermore, sulfide, iron, pH, pE, calcium and total inorganic carbon (TIC) were included in the simulation. The program calculated mineral equilibria to mackinawite, iron sulfides (more stable than mackinawite), iron hydroxides and calcite via saturation indices (SI) by a module incorporating the program PHREEQC (Parkhurst 1995). The measured sulfide and iron profiles are obtained in the simulation output by using a constant SI (=0) for mackinawite and calcite, while a depth dependent SI distribution is applied for the PHREEQC phases ``Pyrite'' and ``Fe(OH)3(a)'', representing a composition and the kinetics of different iron sulfides and iron hydroxides. These SI distributions control the results of sulfide and iron pore water profiles, especially conserving the sulfide profile at the reaction zone during the simulation. The results suggest that phases of iron hydroxides are dissolved, mackinawite is precipitated within, and other iron sulfides are precipitated below the reaction zone. The chemical reactivity of iron hydroxides corresponds to the rate of sulfide production. The system H2O-CO2-CaCO3 is generally successfully maintained during the simulation. Deviations to the measured pH profile suggest that further processes are active which are not included in the simulation yet.

  5. A deep reef in deep trouble

    USGS Publications Warehouse

    Menza, Charles; Kendall, M.; Rogers, C.; Miller, J.

    2007-01-01

    The well-documented degradation of shallower reefs which are often closer to land and more vulnerable to pollution, sewage and other human-related stressors has led to the suggestion that deeper, more remote offshore reefs could possibly serve as sources of coral and fish larvae to replenish the shallower reefs. Yet, the distribution, status, and ecological roles of deep (>30 m) Caribbean reefs are not well known. In this report, an observation of a deep reef which has undergone a recent extensive loss of coral cover is presented. In stark contrast to the typical pattern of coral loss in shallow reefs, the deeper corals were most affected. This report is the first description of such a pattern of coral loss on a deep reef.

  6. Unexpected bismuth concentration profiles in metal-organic vapor phase epitaxy-grown Ga(As{sub 1−x}Bi{sub x})/GaAs superlattices revealed by Z-contrast scanning transmission electron microscopy imaging

    SciTech Connect

    Wood, A. W.; Babcock, S. E.; Guan, Y.; Forghani, K.; Anand, A.; Kuech, T. F.

    2015-03-01

    A set of GaAs{sub 1−x}Bi{sub x}/GaAs multilayer quantum-well structures was deposited by metal-organic vapor phase epitaxy at 390 °C and 420 °C. The precursor fluxes were introduced with the intent of growing discrete and compositionally uniform GaAs{sub 1−x}Bi{sub x} well and GaAs barrier layers in the epitaxial films. High-resolution high-angle annular-dark-field (or “Z-contrast”) scanning transmission electron microscopy imaging revealed concentration profiles that were periodic in the growth direction, but far more complicated in shape than the intended square wave. The observed composition profiles could explain various reports of physical properties measurements that suggest compositional inhomogeneity in GaAs{sub 1−x}Bi{sub x} alloys as they currently are grown.

  7. Characterization of Deep Sea Fish Gut Bacteria with Antagonistic Potential, from Centroscyllium fabricii (Deep Sea Shark).

    PubMed

    Bindiya, E S; Tina, K J; Raghul, Subin S; Bhat, Sarita G

    2015-06-01

    The bacterial isolates from Centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. This study focuses on strain BTSS-3, which showed antimicrobial activity against pathogenic bacteria including Salmonella Typhimurium, Proteus vulgaris, Clostridium perfringens, Staphylococcus aureus, Bacillus cereus, Bacillus circulans, Bacillus macerans and Bacillus pumilus. BTSS3 was subjected to phenotypic characterization using biochemical tests, SEM imaging, exoenzyme profiling and antibiotic susceptibility tests. Comparative 16S rDNA gene sequence analysis indicated that this strain belonged to the genus Bacillus, with high (98%) similarity to 16S rDNA sequences of Bacillus amyloliquefaciens. The chemical nature of the antibacterial substance was identified by treatment with proteolytic enzymes. The antibacterial activity was reduced by the action of these enzymes pointing out its peptide nature. It was observed from the growth and production kinetics that the bacteriocin was produced in the eighth hour of incubation, i.e., during the mid-log growth phase of the bacteria. PMID:25740801

  8. Complex Variability of the Hα Emission Line Profile of the T Tauri Binary System KH 15D: The Influence of Orbital Phase, Occultation by the Circumbinary Disk, and Accretion Phenomena

    NASA Astrophysics Data System (ADS)

    Hamilton, Catrina M.; Johns-Krull, Christopher M.; Mundt, Reinhard; Herbst, William; Winn, Joshua N.

    2012-06-01

    We have obtained 48 high-resolution echelle spectra of the pre-main-sequence eclipsing binary system KH 15D (V582 Mon, P = 48.37 days, e ~ 0.6, MA = 0.6 M ⊙, MB = 0.7 M ⊙). The eclipses are caused by a circumbinary disk (CBD) seen nearly edge on, which at the epoch of these observations completely obscured the orbit of star B and a large portion of the orbit of star A. The spectra were obtained over five contiguous observing seasons from 2001/2002 to 2005/2006 while star A was fully visible, fully occulted, and during several ingress and egress events. The Hα line profile shows dramatic changes in these time series data over timescales ranging from days to years. A fraction of the variations are due to "edge effects" and depend only on the height of star A above or below the razor sharp edge of the occulting disk. Other observed variations depend on the orbital phase: the Hα emission line profile changes from an inverse P-Cygni-type profile during ingress to an enhanced double-peaked profile, with both a blue and a red emission component, during egress. Each of these interpreted variations are complicated by the fact that there is also a chaotic, irregular component present in these profiles. We find that the complex data set can be largely understood in the context of accretion onto the stars from a CBD with gas flows as predicted by the models of eccentric T Tauri binaries put forward by Artymowicz & Lubow, Günther & Kley, and de Val-Borro et al. In particular, our data provide strong support for the pulsed accretion phenomenon, in which enhanced accretion occurs during and after perihelion passage.

  9. COMPLEX VARIABILITY OF THE H{alpha} EMISSION LINE PROFILE OF THE T TAURI BINARY SYSTEM KH 15D: THE INFLUENCE OF ORBITAL PHASE, OCCULTATION BY THE CIRCUMBINARY DISK, AND ACCRETION PHENOMENA

    SciTech Connect

    Hamilton, Catrina M.; Johns-Krull, Christopher M.; Mundt, Reinhard; Herbst, William; Winn, Joshua N. E-mail: cmj@rice.edu E-mail: wherbst@wesleyan.edu

    2012-06-01

    We have obtained 48 high-resolution echelle spectra of the pre-main-sequence eclipsing binary system KH 15D (V582 Mon, P = 48.37 days, e {approx} 0.6, M{sub A} = 0.6 M{sub Sun }, M{sub B} = 0.7 M{sub Sun }). The eclipses are caused by a circumbinary disk (CBD) seen nearly edge on, which at the epoch of these observations completely obscured the orbit of star B and a large portion of the orbit of star A. The spectra were obtained over five contiguous observing seasons from 2001/2002 to 2005/2006 while star A was fully visible, fully occulted, and during several ingress and egress events. The H{alpha} line profile shows dramatic changes in these time series data over timescales ranging from days to years. A fraction of the variations are due to 'edge effects' and depend only on the height of star A above or below the razor sharp edge of the occulting disk. Other observed variations depend on the orbital phase: the H{alpha} emission line profile changes from an inverse P-Cygni-type profile during ingress to an enhanced double-peaked profile, with both a blue and a red emission component, during egress. Each of these interpreted variations are complicated by the fact that there is also a chaotic, irregular component present in these profiles. We find that the complex data set can be largely understood in the context of accretion onto the stars from a CBD with gas flows as predicted by the models of eccentric T Tauri binaries put forward by Artymowicz and Lubow, Guenther and Kley, and de Val-Borro et al. In particular, our data provide strong support for the pulsed accretion phenomenon, in which enhanced accretion occurs during and after perihelion passage.

  10. Deep-diving dinosaurs

    NASA Astrophysics Data System (ADS)

    Hayman, John

    2012-08-01

    Dysbaric bone necrosis demonstrated in ichthyosaurs may be the result of prolonged deep diving rather than rapid ascent to escape predators. The bone lesions show structural and anatomical similarity to those that may occur in human divers and in the deep diving sperm whale, Physeter macrocephalus.

  11. The Deep Space Network

    NASA Technical Reports Server (NTRS)

    1979-01-01

    Deep Space Network progress in flight project support, tracking and data acquisition, research and technology, network engineering, hardware and software implementation, and operations is cited. Topics covered include: tracking and ground based navigation; spacecraft/ground communication; station control and operations technology; ground communications; and deep space stations.

  12. The deep space network

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The progress is reported of Deep Space Network (DSN) research in the following areas: (1) flight project support, (2) spacecraft/ground communications, (3) station control and operations technology, (4) network control and processing, and (5) deep space stations. A description of the DSN functions and facilities is included.

  13. Deep-diving dinosaurs.

    PubMed

    Hayman, John

    2012-08-01

    Dysbaric bone necrosis demonstrated in ichthyosaurs may be the result of prolonged deep diving rather than rapid ascent to escape predators. The bone lesions show structural and anatomical similarity to those that may occur in human divers and in the deep diving sperm whale, Physeter macrocephalus. PMID:22824942

  14. Characterisation of the aroma profiles of different honeys and corresponding flowers using solid-phase microextraction and gas chromatography-mass spectrometry/olfactometry.

    PubMed

    Seisonen, Sirli; Kivima, Evelin; Vene, Kristel

    2015-02-15

    The aroma profiles of thirteen different honey samples from four botanical origins: heather (Calluna vulgaris), raspberry (Rubus idaeus), rape (Brassica napus), alder buckthorn (Frangula alnus) and the blossoms of the four corresponding flowers were investigated to find odour-active compounds exclusively representing specific honeys based on odour-active compounds from the blossoms. Gas-chromatography-mass spectrometry (GC-MS) and gas-chromatography-olfactometry were used to determine and identify the odour-active compounds. Data was analysed using agglomerative hierarchical clustering and correspondence analysis. Honeys from the same botanical origin clustered together; however, none of the identified compounds were exclusive to a particular honey/blossom combination. Heather honey had the flavour profile most different to the others. Isophorone and 2-methylbutyric acid were found only in heather honeys. Heather honey was characterised by having more "sweet" and "candy-like" notes, raspberry honeys had more "green" notes, while alder buckthorn had more "honey" and "floral" notes. PMID:25236195

  15. Presence of monoterpene synthase in four Labiatae species and Solid-Phase Microextraction- Gas chromatography-Mass Spectroscopy analysis of their aroma profiles

    PubMed Central

    Saeidnia, Soodabeh; Gohari, Ahmad Reza; Haddadi, Azita; Amin, Gholamreza; Nikan, Marjan; Hadjiakhoondi, Abbass

    2014-01-01

    Background: The family Lamiaceae (Labiatae) has included some medicinal plants. some monoterpene synthases, including linalool and limonene synthases, have been cloned and functionally characterized from several plants of Labiatae family. Materials and Methods: In this study, presence of linalool and limonene synthases, in four species of Labiatae family including Nepeta cataria, Lavandula angustifolia, Hyssopus officinalis and Salvia sclarea has been determined by molecular biological techniques together with the Head space SPME – GC-MS analysis of the aroma profile of these species. Results: Indicated that none of the plant species produced distinguishable bands with primer pairs related to d-limonene synthase. Distinguishable bands around 1800 bp in cDNA samples of L. angustifolia, H. officinalis and S. sclarea were observed regarding to the presence of linalool synthase. Head space SPME-GC-MS analysis of the aroma profiles of the above-mentioned plants showed that linalool (31.0%), linalyl acetate (18.2%), were found as the major compounds of L. angustifolia, while geraniol (5.5%), nerol (34.0%) and α- citral (52.0%) were identified as the main compounds of the N. cataria. The major components of H. officinalis and S. sclarea oils were determined as cis-pinocamphone (57.3%), and linalool (19.0%), linalyl acetate (51.5%), respectively. Conclusion: H. officinalis was rich of cyclic monoterpenes, L. angustifolia, N. cataria and S. sclarea showed considerable amount of linear monoterpenes. The aroma profile of the above-mentioned plants contained low concentration of sesquiterpenes except N. cataria, which indicated no sesquiterpene. The profiles of the main components of these plants are in agreement with molecular assays. PMID:24761118

  16. [Deep neck infections].

    PubMed

    Nowak, Katarzyna; Szyfter, Witold

    2006-01-01

    Deep neck infection is relatively rare but potentially life threatening complication of common oropharyngeal infections. This retrospective study was aimed at analyzing the occurrence of complications, diagnostic methods and proper management of deep neck infection. A review was conducted in 32 cases who were diagnosed as having deep neck infection from 1995 to 2005. The causes of deep neck infections were tonsillitis (16 cases), tooth diseases (6 cases), paratonsillar abscess (4 cases), parotitis (1 case), pussy lymphonodes after tonsillectomy (2 cases), pussy congenital neck cyst (1 case), chronic otitis media (1 case), parotitis (1 case), foreign body of the esophagus (1 case). All the puss bacterial cultivation were positive. All the patients were treated by different ways of chirurgical drainage and use of large dosage of antibiotics. Deep neck infection should be suspected in patients with long lasting fever and painful swelling of the neck and treatment should begin quick as possible. PMID:17152800

  17. Advanced transponders for deep space applications

    NASA Technical Reports Server (NTRS)

    Nguyen, Tien M.; Kayalar, Selahattin; Yeh, Hen-Geul; Kyriacou, Charles

    1993-01-01

    Three architectures for advanced deep space transponders are proposed. The architectures possess various digital techniques such as fast Fourier transform (FFT), digital phase-locked loop (PLL), and digital sideband aided carrier detection with analog or digital turn-around ranging. Preliminary results on the design and conceptual implementation are presented. Modifications to the command detector unit (CDU) are also presented.

  18. Development of an achiral supercritical fluid chromatography method with ultraviolet absorbance and mass spectrometric detection for impurity profiling of drug candidates. Part II. Selection of an orthogonal set of stationary phases.

    PubMed

    Lemasson, Elise; Bertin, Sophie; Hennig, Philippe; Boiteux, Hélène; Lesellier, Eric; West, Caroline

    2015-08-21

    Impurity profiling of organic products that are synthesized as possible drug candidates requires complementary analytical methods to ensure that all impurities are identified. Supercritical fluid chromatography (SFC) is a very useful tool to achieve this objective, as an adequate selection of stationary phases can provide orthogonal separations so as to maximize the chances to see all impurities. In this series of papers, we have developed a method for achiral SFC-MS profiling of drug candidates, based on a selection of 160 analytes issued from Servier Research Laboratories. In the first part of this study, focusing on mobile phase selection, a gradient elution with carbon dioxide and methanol comprising 2% water and 20mM ammonium acetate proved to be the best in terms of chromatographic performance, while also providing good MS response [1]. The objective of this second part was the selection of an orthogonal set of ultra-high performance stationary phases, that was carried out in two steps. Firstly, a reduced set of analytes (20) was used to screen 23 columns. The columns selected were all 1.7-2.5μm fully porous or 2.6-2.7μm superficially porous particles, with a variety of stationary phase chemistries. Derringer desirability functions were used to rank the columns according to retention window, column efficiency evaluated with peak width of selected analytes, and the proportion of analytes successfully eluted with good peak shapes. The columns providing the worst performances were thus eliminated and a shorter selection of columns (11) was obtained. Secondly, based on 160 tested analytes, the 11 columns were ranked again. The retention data obtained on these columns were then compared to define a reduced set of the best columns providing the greatest orthogonality, to maximize the chances to see all impurities within a limited number of runs. Two high-performance columns were thus selected: ACQUITY UPC(2) HSS C18 SB and Nucleoshell HILIC. PMID:26195036

  19. Deep Moonquakes: Remaining Problems

    NASA Technical Reports Server (NTRS)

    Nakamura, Y.

    2004-01-01

    We have recently reexamined more than 9000 United States previously unidentified seismic events catalogued during the Apollo landing missions and positively identified for the first time about 30 deep moonquake nests on the far side of the Moon. Although only a few of them are currently locatable, the relative arrival times among stations for the rest and presence or absence of seismic signals at particular stations suggest that either (a) the region within about $40\\deg$ of the antipode is aseismic or (b) the deep interior of the Moon severely attenuates or deflects seismic waves. Aside from the obvious question of how to distinguish between such hypothetical models, this effort raised several more general questions concerning the use of deep moonquake signals to infer the structure and dynamics of the deep interior of the Moon. Among more important ones are: (1) How reliable are the seismic arrival picks from which to compute the seismic velocity variations in the Moon? (2) How do the possible lateral variations in seismic velocity affect the computed radial variation in seismic velocity at depth? (3) Can we tell more about the distribution and mechanism of deep moonquakes from the newly expanded database of identified deep moonquakes? Questions (1) and (2) are especially important because the inferred deep internal structure of the Moon depends critically on their answers. Answering these questions may demand additional data collected on future lunar missions, but some may be resolved with further examination of the existing data.

  20. Seafloor bathymetry in deep and shallow water marine CSEM responses of Nigerian Niger Delta oil field: Effects and corrections

    NASA Astrophysics Data System (ADS)

    Folorunso, Adetayo Femi; Li, Yuguo

    2015-12-01

    Topography distortions in bathymetrically acquired marine Controlled-Source Electromagnetic (mCSEM) responses are capable of misleading interpretation to the presence or absence of the target if not corrected for. For this reason, the effects and correction of bathymetry distortions on the deep and shallow seafloor mCSEM responses of the Niger Delta Oil province were examined in this paper. Marine CSEM response of the Niger Delta geological structure was modelled by using a 2.5D adaptive finite element forward modelling code. In both the deep water and shallow water cases, the bathymetry distortions in the electric field amplitude and phase were found to get smaller with increasing Tx-Rx offsets and contain short-wavelength components in the amplitude curves which persist at all Tx-Rx offsets. In the deep water, topographic effects on the reservoir signatures are not significant, but as water depth reduces, bathymetric distortions become more significant as a result of the airwave effects, masking the target signatures. The correction technique produces a good agreement between the flat-seafloor reservoir model and its equivalent bathymetric model in deep water at 0.25 Hz, while in shallow water, the corrected response only shows good agreement at shorter offsets but becomes complicated at longer offsets due to airwave effects. Transmission frequency was extended above and below 0.25 Hz in the frequency spectrum and the correction method applied. The bathymetry correction at higher frequency (1.75 Hz) is not effective in removing the topographic effects in either deep or shallow water. At 0.05 Hz for both seafloor scenarios, we obtained the best corrected amplitude profiles, removing completely the distortions from both topographic undulation and airwave effects in the shallow water model. Overall, the work shows that the correction technique is effective in reducing bathymetric effects in deep water at medium frequency and in both deep and shallow waters at a low

  1. The deep Ionian Basin revisited

    NASA Astrophysics Data System (ADS)

    Tugend, Julie; Chamot-Rooke, Nicolas; Arsenikos, Stavros; Frizon de Lamotte, Dominique; Blanpied, Christian

    2016-04-01

    The deep Eastern Mediterranean Basins (Ionian and Herodotus) are characterized by thick sedimentary sequences overlying an extremely thinned basement evidenced from different geophysical methods. Yet, the nature of the crust (continental or oceanic) and the timing of the extreme crustal and lithosphere thinning in the different sub-basins remain highly controversial, casting doubts on the tectonic setting related to the formation of this segment of the North Gondwana paleo-margin. We focus on the Ionian Basin located at the western termination of the Eastern Mediterranean with the aim of identifying, characterizing and mapping the deepest sedimentary sequences. We present tentative age correlations relying on calibrations and observations from the surrounding margins and basins (Malta shelf and Escarpment, Cyrenaica margin, Sirte Basin, Apulian Platform). Two-ship deep refraction seismic data (Expanding Spread Profiles from the PASIPHAE cruise) combined with reprocessed reflection data (from the ARCHIMEDE survey) enabled us to present a homogeneous seismic stratigraphy across the basin and to investigate the velocity structure of its basement. Based on our results, and on a review of geological and geophysical observations, we suggest an Upper Triassic-Early Dogger age for the formation of the deep Ionian Basin. The nature of the underlying basement remains uncertain, both highly-thinned continental and slow-spreading type oceanic crust being compatible with the available constraints. The narrow size and relatively short-lived evolution of the Ionian Basin lead us to suggest that it is more likely the remnant of an immature oceanic basin than of a stable oceanic domain. Eventually, upscaling these results at the scale of the Eastern Mediterranean Basins highlights the complex interaction observed between two propagating oceans: The Central Atlantic and Neo-Tethys.

  2. Deep subsurface microbial processes

    USGS Publications Warehouse

    Lovley, D.R.; Chapelle, F.H.

    1995-01-01

    Information on the microbiology of the deep subsurface is necessary in order to understand the factors controlling the rate and extent of the microbially catalyzed redox reactions that influence the geophysical properties of these environments. Furthermore, there is an increasing threat that deep aquifers, an important drinking water resource, may be contaminated by man's activities, and there is a need to predict the extent to which microbial activity may remediate such contamination. Metabolically active microorganisms can be recovered from a diversity of deep subsurface environments. The available evidence suggests that these microorganisms are responsible for catalyzing the oxidation of organic matter coupled to a variety of electron acceptors just as microorganisms do in surface sediments, but at much slower rates. The technical difficulties in aseptically sampling deep subsurface sediments and the fact that microbial processes in laboratory incubations of deep subsurface material often do not mimic in situ processes frequently necessitate that microbial activity in the deep subsurface be inferred through nonmicrobiological analyses of ground water. These approaches include measurements of dissolved H2, which can predict the predominant microbially catalyzed redox reactions in aquifers, as well as geochemical and groundwater flow modeling, which can be used to estimate the rates of microbial processes. Microorganisms recovered from the deep subsurface have the potential to affect the fate of toxic organics and inorganic contaminants in groundwater. Microbial activity also greatly influences 1 the chemistry of many pristine groundwaters and contributes to such phenomena as porosity development in carbonate aquifers, accumulation of undesirably high concentrations of dissolved iron, and production of methane and hydrogen sulfide. Although the last decade has seen a dramatic increase in interest in deep subsurface microbiology, in comparison with the study of

  3. Dispersion retrieval from multi-level ultra-deep reactive-ion-etched microstructures for terahertz slow-wave circuits

    SciTech Connect

    Baik, Chan-Wook Young Ahn, Ho; Kim, Yongsung; Lee, Jooho; Hong, Seogwoo; Hee Choi, Jun; Kim, Sunil; Hun Lee, Sang; Min Kim, Jong; Hwang, Sungwoo; Yeon Jun, So; Yu, SeGi; Lawrence Ives, R.

    2014-01-13

    A multi-level microstructure is proposed for terahertz slow-wave circuits, with dispersion relation retrieved by scattering parameter measurements. The measured return loss shows strong resonances above the cutoff with negligible phase shifts compared with finite element analysis. Splitting the circuit into multi levels enables a low aspect ratio configuration that alleviates the loading effect of deep-reactive-ion etching on silicon wafers. This makes it easier to achieve flat-etched bottom and smooth sidewall profiles. The dispersion retrieved from the measurement, therefore, corresponds well to the theoretical estimation. The result provides a straightforward way to the precise determination of dispersions in terahertz vacuum electronics.

  4. Deep Space 1 Technology Demonstrator

    NASA Technical Reports Server (NTRS)

    1998-01-01

    The completely assembled Deep Space 1 (DS-1) technology demonstrator spacecraft. The DS-1 spacecraft incorporates a number of advanced technology concepts in its mission, but none so 'high profile' as its Ion propulsion system. The name itself evokes visions of Star Trek and science fiction fantasy, although the idea actually dates from the 1950s. However, unlike the 'Warp Drive' propulsion system that zings the fictional starship Enterprise across the cosmos in minutes, the almost imperceptible thrust from the ion propulsion system is equivalent to the pressure exerted by a sheet of paper held in the palm of your hand. The ion engine is very slow to pick up speed, but over the long haul it can deliver 10 times as much thrust per pound of fuel as more traditional rockets. Unlike the fireworks of most chemical rockets using solid or liquid fuels, the ion drive emits only an eerie blue glow as ionized (electrically charged) atoms of xenon are pushed out of the engine. Xenon is the same gas found in photo flash tubes and many lighthouse bulbs. Deep Space 1 was launched in October 1998 as part of NASA's New Millennium Program, which is managed by JPL for NASA's Office of Space Science, Washington, DC. The California Institute of Technology in Pasadena manages JPL for NASA.

  5. Deep venous reconstructive surgery.

    PubMed

    Maleti, Oscar; Lugli, Marzia; Tripathi, Ramesh K

    2015-03-01

    Surgical correction of deep venous reflux is a valuable adjunct in treatment of selected patient with lower limb venous ulcer. Deep venous obstruction and superficial reflux is must be corrected first. Sustained venous ulcer healing and reduced ambulatory venous hypertension can be achieved in patients with both primary and secondary deep venous insufficiency. When direct valve repair is possible, valvuloplasty is the best option, but when this is not feasible, other techniques can be used, including femoral vein transposition into the great saphenous vein, vein valve transplant, neovalve construction, or nonautologous artificial venous valve. PMID:26358308

  6. Deep Downhole Seismic Testing at the Waste Treatment Plant Site, Hanford, WA. Volume V S-Wave Measurements in Borehole C4996 Seismic Records, Wave-Arrival Identifications and Interpreted S-Wave Velocity Profile.

    SciTech Connect

    Stokoe, Kenneth H.; Li, Song Cheng; Cox, Brady R.; Menq, Farn-Yuh

    2007-06-06

    Velocity measurements in shallow sediments from ground surface to approximately 370 to 400 feet bgs were collected by Redpath Geophysics using impulsive S- and P-wave seismic sources (Redpath 2007). Measurements below this depth within basalt and sedimentary interbeds were made by UTA between October and December 2006 using the T-Rex vibratory seismic source in each of the three boreholes. Results of these measurements including seismic records, wave-arrival identifications and interpreted velocity profiles are presented in the following six volumes: I. P-Wave Measurements in Borehole C4993 II. P-Wave Measurements in Borehole C4996 III. P-Wave Measurements in Borehole C4997 IV. S-Wave Measurements in Borehole C4993 V. S-Wave Measurements in Borehole C4996 VI. S-Wave Measurements in Borehole C4997 In this volume (V), all S-wave measurements are presented that were performed in Borehole C4996 at the WTP with T-Rex as the seismic source and the Lawrence Berkeley National Laboratory (LBNL) 3-D wireline geophone as the at-depth borehole receiver.

  7. Deep Downhole Seismic Testing at the Waste Treatment Plant Site, Hanford, WA. Volume VI S-Wave Measurements in Borehole C4997 Seismic Records, Wave-Arrival Identifications and Interpreted S-Wave Velocity Profile.

    SciTech Connect

    Stokoe, Kenneth H.; Li, Song Cheng; Cox, Brady R.; Menq, Farn-Yuh

    2007-06-06

    Velocity measurements in shallow sedimen