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Sample records for del nerium oleander

  1. Larvicidal Activity of Nerium oleander against Larvae West Nile Vector Mosquito Culex pipiens (Diptera: Culicidae)

    PubMed Central

    El-Akhal, Fouad; Guemmouh, Raja; Ez Zoubi, Yassine; El Ouali Lalami, Abdelhakim

    2015-01-01

    Background. Outbreaks of the West Nile virus infection were reported in Morocco in 1996, 2003, and 2010. Culex pipiens was strongly suspected as the vector responsible for transmission. In the North center of Morocco, this species has developed resistance to synthetic insecticides. There is an urgent need to find alternatives to the insecticides as natural biocides. Objective. In this work, the insecticidal activity of the extract of the local plant Nerium oleander, which has never been tested before in the North center of Morocco, was studied on larval stages 3 and 4 of Culex pipiens. Methods. Biological tests were realized according to a methodology inspired from standard World Health Organization protocol. The mortality values were determined after 24 h of exposure and LC50 and LC90 values were calculated. Results. The extract had toxic effects on the larvae of culicid mosquitoes. The ethanolic extract of Nerium oleander applied against the larvae of Culex pipiens has given the lethal concentrations LC50 and LC90 in the order of 57.57 mg/mL and 166.35 mg/mL, respectively. Conclusion. This investigation indicates that N. oleander could serve as a potential larvicidal, effective natural biocide against mosquito larvae, particularly Culex pipiens. PMID:26640701

  2. Synchrotron radiation X-ray fluorescence analysis of trace elements in Nerium oleander for pollution monitoring

    NASA Astrophysics Data System (ADS)

    de Jesus, E. F. O.; Simabuco, S. M.; dos Anjos, M. J.; Lopes, R. T.

    2000-07-01

    This works describes the use of synchrotron radiation fluorescence analysis as a technique for monitoring trace elements in bio-indicators for environmental pollution control. The analyses were performed on leaves of Nerium oleander collected in streets with different levels of traffic flow in Rio de Janeiro, Brazil, with one sample from a rural zone. The leaves were collected from adult trees in December and April. The measurement was made with a white beam of synchrotron radiation calibrated with thin samples from MicroMatter. The results indicate that some metals such as Ti, V, Fe and Zn have major content in samples that were collected in places with a high traffic flow, even in the leaves that have been washed. The levels of Mn, Co, Cu and Ni did not show significant differences between the samples. The Pb level also did not vary significantly. This was expected because in Brazil gasoline without Pb has been used for many years. The results seem to indicate that the leaves from Nerium oleander absorb metals from the atmosphere and may be used as an environmental indicator.

  3. Evaluation of skeletal muscle relaxant activity of aqueous extract of Nerium oleander flowers in Albino rats

    PubMed Central

    Tirumalasetti, Jayasree; Patel, Maulik; Shaikh, Ubedulla; Harini, K.; Shankar, J.

    2015-01-01

    Objectives: Nerium oleander is traditionally used in various diseases because of its medicinal properties. One of its uses is in musculoskeletal disorder. The aim of the study was to evaluate the skeletal muscle relaxant activity of the aqueous extract of Nerium oleander flowers (AENOF) in albino rats in comparison with diazepam. Materials and Methods: A total of 20 Swiss albino rats aged 67 weeks, of either sex, weighing about 100150 g, were taken, and after acute toxicity studies two different doses were selected. The animals were divided into four different groups. The first group was kept as the control (normal saline), second as the standard (diazepam) and the remaining two groups as Test I and Test II, and given different doses of the AENOF. Skeletal muscle relaxant activity (motor coordination) on Rotarod and locomotor activity on photoactometer was performed. Statistical analysis was carried out by using analysis of variance, followed by Dunnett's multiple comparison tests. Results: The result from the Actophotometer test and Rotarod test showed that the extract of AENOF significantly reduced (P < 0.05) the motor coordination of the tested animals. Conclusions: Our data indicates that AENOF possesses skeletal muscle relaxant activities. PMID:26288474

  4. A hydroalcoholic extract from the leaves of Nerium oleander inhibits glycolysis and induces selective killing of lung cancer cells.

    PubMed

    Caldern-Montao, Jos Manuel; Burgos-Morn, Estefana; Orta, Manuel Luis; Mateos, Santiago; Lpez-Lzaro, Miguel

    2013-08-01

    Recent evidence suggests that cardiac glycosides might be used for the treatment of cancer. The ornamental shrub Nerium oleander has been used in traditional medicine for treating several disorders including cancer, and extracts from the leaves of this plant have already entered phase I clinical trials. In this communication, we have prepared a hydroalcoholic extract from the leaves of Nerium oleander (containing 4.75 0.32 % of cardenolides) and have assessed its cytotoxic activity in A549 lung cancer cells vs. MRC5 nonmalignant lung fibroblasts. The results showed that the cytotoxicity of the Nerium oleander extract against the cancer cell line was significantly higher than that against the nonmalignant cell line, with a potency and selectivity similar to those of the anticancer drug cisplatin. Pretreatment of A549 cells with the antioxidants N-acetylcysteine and catalase slightly prevented the cytotoxicity of the extract, therefore suggesting that the formation of reactive oxygen species participates in its cytotoxic activity but does not play a major role. Nerium oleander extract-induced cytotoxicity and DNA damage (gamma-H2AX focus formation) were slightly higher in cells lacking BRCA2 (deficient in homologous recombination repair) than in parental cells; this indicates that the induction of DNA damage may also play a role in the cytotoxicity of the extract. Nerium oleander extract induced a marked inhibition of glycolysis (glucose consumption and lactate production) in A549 cells, comparable to that of the glycolysis inhibitor dichloroacetate (currently in clinical development for cancer therapy). Because platinum compounds are widely used in the treatment of lung cancer, we tested the cytotoxicity of several combinations of cisplatin with the extract and found a moderate synergism when Nerium oleander extract was administered after cisplatin but a moderate antagonism when it was added before cisplatin. Our results suggest that extracts from Nerium oleander might induce anticancer effects in patients with lung cancer and support their possible advancement into phase II clinical trials for the treatment of this type of cancer. PMID:23824549

  5. Antifungal activity of nettle (Urtica dioica L.), colocynth (Citrullus colocynthis L. Schrad), oleander (Nerium oleander L.) and konar (Ziziphus spina-christi L.) extracts on plants pathogenic fungi.

    PubMed

    Hadizadeh, I; Peivastegan, B; Kolahi, M

    2009-01-01

    Anti-mycotic activity of the ethanol extracts from Nettle (Urtica dioica L.), Colocynth (Citrullus colocynthis L. Schrad), Konar (Ziziphus spina-christi L.) and Oleander (Nerium oleander L.) floral parts were screened in vitro against four important plant pathogenic fungi viz.; Alternaria alternate, Fusarium oxysporum, Fusarium solani and Rizoctonia solani using agar dilution bioassay. Extracts showed antifungal activity against all the tested fungi. Among the plants, Nettle and Colocynth were the most effective against A. alternate and R. solani while Oleander possesses the best inhibition on F. oxysporum and F. solani. Konar was the most effective extract by reducing the growth of Rizoctonia solani than other fungi. These results showed that extracts could be considered suitable alternatives to chemical additives for the control of fungal diseases in plants. PMID:19579919

  6. New sightings of Glyphodes onychinalis (Guene) (Pyraloidea: Crambidae: Spilomelinae), a recent arrival to the United States, and description of the larva reared on oleander (Nerium sp.) in California

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In 2007 and 2008, Don Sterba of Culver City, Los Angeles Co., observed the adults of Glyphodes onychinalis (Guene), a non-native crambid, in large numbers. It was first reported new to the United States from Newport Beach, California in 2000 where it was reared from oleander (Nerium sp.). The la...

  7. Metal uptake and distribution in cultured seedlings of Nerium oleander L. (Apocynaceae) from the Ro Tinto (Huelva, Spain).

    PubMed

    Franco, Alejandro; Rufo, Lourdes; Zuluaga, Javier; de la Fuente, Vicenta

    2013-10-01

    Nerium oleander L. (Apocynaceae) is a micro-nano phanerophyte that grows in the riverbanks of the Ro Tinto basin (Southwest Iberian Peninsula). The waters and soils of the Ro Tinto area are highly acidic and have high concentrations of heavy metals. In this environment, N. oleander naturally grows in both extreme acidic (EA) and less extreme acidic (LEA) water courses, excluding, and bioindicating certain metals. In this work, we compared and evaluated the accumulation preferences and capacities, the distribution and processes of biomineralization of metals (Fe, Cu, Zn, Mn, Mg, Ca) in the first stages of growth of EA and LEA oleanders by means of inductively coupled plasma-mass spectrometry, scanning electron microscopy, and energy dispersive X-ray analyzer analysis. Seeds from both environments were grown and treated with a self-made solution simulating the most extreme red waters from the Ro Tinto. LEA plants drastically reduces the metal uptake at the beginning, but later reactivates the uptake reaching concentration values in the same range as the EA plants. The results showed high Mn, Zn and Mg concentrations, accumulation of Fe and Cu in plants from both environments, differing from the metal concentrations of field-grown oleanders. Iron bioformations with traces of other metals were present inside and over epidermal cells and inside vascular cells of stems and roots. They were absent of leaves. The accumulation properties of N. oleander in its early stages of development make it a species to take in consideration in phytoremediation processes but optimized conditions are needed to ensure enough biomass production. PMID:23892697

  8. Evaluation of leaf aqueous extract and synthesized silver nanoparticles using Nerium oleander against Anopheles stephensi (Diptera: Culicidae).

    PubMed

    Roni, Mathath; Murugan, Kadarkarai; Panneerselvam, Chellasamy; Subramaniam, Jayapal; Hwang, Jiang-Shiou

    2013-03-01

    Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and ecofriendly reducing and capping agents. The present study was carried out to establish the larvicidal activity of synthesized silver nanoparticles (AgNPs) using leaf extract of Nerium oleander (Apocynaceae) against the first to fourth instar larvae and pupae of malaria vector, Anopheles stephensi (Diptera: Culicidae). Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by the aqueous extract of the plant parts to generate extremely stable silver nanoparticles in water. The results were recorded from UV-Vis spectrum, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) spectroscopy analysis. The production of the AgNPs synthesized using leaf extract of N. oleander was evaluated through a UV-Vis spectrophotometer in a wavelength range of 200 to 700nm. This revealed a peak at 440nm in N. oleander leaf extracts, indicating the production of AgNPs. The FTIR spectra of AgNPs exhibited prominent peaks at 509.12cm(-1) (C-H bend alkenes), 1,077.05cm(-1) (C-O stretch alcohols), 1,600.63cm(-1) (N-H bend amines), 2,736.49 and 2,479.04cm(-1) (O-H stretch carboxylic acids), and 3,415.31cm(-1) (N-H stretching due to amines group). An SEM micrograph showed 20-35-nm-size aggregates of spherical- and cubic-shaped nanoparticles. EDX showed the complete chemical composition of the synthesized nanoparticles of silver. Larvicidal activity of aqueous leaf extract of N. oleander and synthesized AgNPs was carried out against Anopheles stephensi, and the results showed that the highest larval mortality was found in the synthesized AgNPs against the first to fourth instar larvae and pupae of Anopheles stephensi with the following values: LC(50) of instar larvae 20.60, 24.90, 28.22, and 33.99ppm; LC(90) of instar larvae 41.62, 50.33, 57.78, and 68.41ppm; and LC(50) and LC(90) of pupae 39.55 and 79.10ppm, respectively. The aqueous leaf extract exhibited larval toxicity against the first to fourth instar larvae and pupae of Anopheles stephensi with the following values: LC(50) of instar larvae 232.90, 273.71, 318.94, and 369.96ppm; LC(90) of instar larvae 455.95, 563.10, 639.86, and 730.30ppm; and LC(50) and LC(90) of pupae 426.01 and 805.13ppm, respectively. The chi-square value was significant at p?oleander to synthesize silver nanoparticles is a rapid, environmentally safer, and greener approach for mosquito control. This could lead us to a new possibility in vector-control strategy. PMID:23239092

  9. A case of Mobitz type II atrioventricular block due to Nerium oleander poisoning successfully managed with digoxin-specific Fab antibody fragments.

    PubMed

    Tatl?su, Mustafa Adem; ekirdeki, Elif ?jlal; Akyz, ?kr; Nurkalem, Zekeriya

    2015-10-01

    Nerium oleander is a popular ornamental plant grown in many tropical and subtropical countries and in the Mediterranean region. It is dangerous because it has been shown to contain several types of cardiac glycosides, and hence can cause cardiac arrhythmias resembling digoxin in their toxicologic manifestations. We report a patient presenting to our hospital with Mobitz type II atrioventricular block after drinking herbal tea prepared from oleander leaves. Three hours after admission, a 200-mg empiric dose of digoxin-specific Fab antibody fragments was administered intravenously over 30 minutes. A 12-lead electrocardiogram (ECG) revealed sinus rhythm at the end of infusion. After 72 hours, the patient was discharged without any symptoms. PMID:26536992

  10. Change in Uptake, Transport and Accumulation of Ions in Nerium oleander (Rosebay) as Affected by Different Nitrogen Sources and Salinity

    PubMed Central

    Abdolzadeh, Ahmad; Shima, Kazuto; Lambers, Hans; Chiba, Kyozo

    2008-01-01

    Background and Aims The source of nitrogen plays an important role in salt tolerance of plants. In this study, the effects of NaCl on net uptake, accumulation and transport of ions were investigated in Nerium oleander with ammonium or nitrate as the nitrogen source in order to analyse differences in uptake and cycling of ions within plants. Methods Plants were grown in a greenhouse in hydroponics under different salt treatments (control vs. 100 mm NaCl) with ammonium or nitrate as the nitrogen source, and changes in ion concentration in plants, xylem sap exuded from roots and stems, and phloem sap were determined. Key Results Plant weight, leaf area and photosynthetic rate showed a higher salt tolerance of nitrate-fed plants compared with that of ammonium-fed plants. The total amount of Na+ transported in the xylem in roots, accumulated in the shoot and retranslocated in the phloem of ammonium-fed plants under salt treatment was 18, 19 and 27 times more, respectively, than that of nitrate-treated plants. However, the amount of Na+ accumulated in roots in nitrate-fed plants was about 15 times higher than that in ammonium-fed plants. Similarly, Cl? transport via the xylem to the shoot and its retranslocation via the phloem (Cl? cycling) were far greater with ammonium treatment than with nitrate treatment under conditions of salinity. The uptake and accumulation of K+ in shoots decreased more due to salinity in ammonium-fed plants compared with nitrate-fed plants. In contrast, K+ cycling in shoots increased due to salinity, with higher rates in the ammonium-treated plants. Conclusions The faster growth of nitrate-fed plants under conditions of salinity was associated with a lower transport and accumulation of Na+ and Cl? in the shoot, whereas in ammonium-fed plants accumulation and cycling of Na+ and Cl? in shoots probably caused harmful effects and reduced growth of plants. PMID:18772147

  11. Zeaxanthin and the Heat Dissipation of Excess Light Energy in Nerium oleander Exposed to a Combination of High Light and Water Stress 1

    PubMed Central

    Demmig, Barbara; Winter, Klaus; Krger, Almuth; Czygan, Franz-Christian

    1988-01-01

    Upon termination of watering of plants of Nerium oleander exposed to high light, photochemical efficiency became reduced as leaf water content decreased. Evidence is presented that this type of photoinhibition reflects to a substantial degree radiationless dissipation of excitation energy, probably mediated by the carotenoid zeaxanthin. During the imposition of water stress, the zeaxanthin content of leaves increased at the expense of violaxanthin and ?-carotene as a water deficit developed over a period of several days. The increase in zeaxanthin content was linearly related to an increase in the rate of radiationless energy dissipation in the antenna chlorophyll as calculated from the characteristics of chlorophyll a fluorescence measured with a pulse amplitude modulated fluorometer at room temperature. The increase in the rate of radiationless dissipation was also linearly related to a decrease in PSII photochemical efficiency as indicated by the ratio of variable to maximum fluorescence. Leaves of well-watered shade plants of N. oleander exposed to strong light showed a similar increase in zeaxanthin content as sun leaves of the same species subjected to drought in strong light. Shade leaves possessed the same capacity as sun leaves to form zeaxanthin at the expense of both violaxanthin and ?-carotene. The resistance of this species to the destructive effects of excess light appears to be related to interconversions between ?-carotene and the three carotenoids of the xanthophyll cycle. PMID:16666096

  12. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (nae-8)

    PubMed Central

    Benson, Kathleen F; Newman, Robert A; Jensen, Gitte S

    2015-01-01

    Objective The goal for this study was to evaluate the effects of an Aloe vera-based Nerium oleander extract (NAE-8), compared to an extract of A. vera gel alone (ALOE), and to an aqueous extract of N. oleander (AQ-NOE) in bioassays pertaining to dermatologic potential with respect to antioxidant protection, anti-inflammatory effects, and cytokine profiles in vitro. Methods Cellular antioxidant protection was evaluated in three separate bioassays: The cellular antioxidant protection of erythrocytes (CAP-e) assay, protection of cellular viability and prevention of apoptosis, and protection of intracellular reduced glutathione levels, where the last two assays were performed using human primary dermal fibroblasts. Reduction of intracellular formation of reactive oxygen species (ROS) was tested using polymorphonuclear cells in the absence and presence of oxidative stress. Changes to cytokine and chemokine profiles when whole blood cells and human primary dermal fibroblasts were exposed to test products were determined using a 40-plex Luminex array as a method for exploring the potential cross-talk between circulating and skin-resident cells. Results The NAE-8 provided significantly better antioxidant protection in the CAP-e bioassay than AQ-NOE. NAE-8 and AQ-NOE both protected cellular viability and intracellular reduced glutathione, and reduced the ROS formation significantly when compared to control cells, both under inflamed and neutral culture conditions. ALOE showed minimal effect in these bioassays. In contrast to the NAE-8, the AQ-NOE showed induction of inflammation in the whole blood cultures, as evidenced by the high induction of CD69 expression and secretion of a number of inflammatory cytokines. The treatment of dermal fibroblasts with NAE-8 resulted in selective secretion of cytokines involved in collagen and hyaluronan production as well as re-epithelialization during wound healing. Conclusion NAE-8, a novel component of a commercial cosmetic product, showed beneficial antioxidant protection in several cellular models, without the induction of leukocyte activation and secretion of inflammatory cytokines. The biological efficacy of NAE-8 was unique from both ALOE and AQ-NOE. PMID:26005354

  13. The effect of urban ground cover on microclimate, growth and leaf gas exchange of oleander in Phoenix, Arizona

    NASA Astrophysics Data System (ADS)

    Mueller, Erin C.; Day, Thomas A.

    2005-03-01

    We assessed how small patches of contrasting urban ground cover [mesiscape (turf), xeriscape (gravel), concrete, and asphalt] altered the microclimate and performance of adjacent oleander (Nerium oleander L.) plants in Phoenix, Arizona during fall/winter (September February) and spring/summer (March September). Ground-cover and oleander canopy surface temperatures, canopy air temperatures and pot soil temperatures tended to be lowest in the mesiscape and highest in the asphalt and concrete. Canopy air vapor pressure deficits were lowest in the mesiscape and highest in the asphalt plot. Rates of net photosynthesis of all oleander plants were highest in October and May, and declined through mid-summer (June July), when rates tended to be highest in the cooler mesiscape, particularly when water was limiting. During fall/winter, oleanders in the mesiscape produced 20% less biomass, 13% less leaf area, and had 12% lower relative growth rates (RG) than those in the other ground covers. Lower nighttime temperatures in the mesiscape in December led to oleander frost damage. During spring/summer, oleanders in the mesiscape produced 11% more biomass, 16% more leaf area, and had 3% higher RG than those in the other cover types. The effects of urban ground cover on oleander performance were season-specific; while oleander growth was greatest in the mesiscape during spring/summer, it was lowest during fall/winter and these plants experienced frost damage. Because all oleander plants produced >10 times as much biomass during the spring/summer, on an annual basis oleanders in the mesiscape produced 5 11% more biomass than plants in the warmer ground covers.

  14. The effect of urban ground cover on microclimate, growth and leaf gas exchange of oleander in Phoenix, Arizona.

    PubMed

    Mueller, Erin C; Day, Thomas A

    2005-03-01

    We assessed how small patches of contrasting urban ground cover [mesiscape (turf), xeriscape (gravel), concrete, and asphalt] altered the microclimate and performance of adjacent oleander (Nerium oleander L.) plants in Phoenix, Arizona during fall/winter (September-February) and spring/summer (March-September). Ground-cover and oleander canopy surface temperatures, canopy air temperatures and pot soil temperatures tended to be lowest in the mesiscape and highest in the asphalt and concrete. Canopy air vapor pressure deficits were lowest in the mesiscape and highest in the asphalt plot. Rates of net photosynthesis of all oleander plants were highest in October and May, and declined through mid-summer (June-July), when rates tended to be highest in the cooler mesiscape, particularly when water was limiting. During fall/winter, oleanders in the mesiscape produced 20% less biomass, 13% less leaf area, and had 12% lower relative growth rates (R(G)) than those in the other ground covers. Lower nighttime temperatures in the mesiscape in December led to oleander frost damage. During spring/summer, oleanders in the mesiscape produced 11% more biomass, 16% more leaf area, and had 3% higher R(G) than those in the other cover types. The effects of urban ground cover on oleander performance were season-specific; while oleander growth was greatest in the mesiscape during spring/summer, it was lowest during fall/winter and these plants experienced frost damage. Because all oleander plants produced >10 times as much biomass during the spring/summer, on an annual basis oleanders in the mesiscape produced 5-11% more biomass than plants in the warmer ground covers. PMID:15726447

  15. Nerium oleander indirect leaf photosynthesis and light harvesting reductions after clipping injury or Spodoptera eridania herbivory: High sensitivity to injury

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Variable indirect photosynthetic rate (Pn) responses occur on injured leaves after insect herbivory. It is important to understand factors that influence indirect Pn reductions after injury. The current study examines the relationship between gas exchange and chlorophyll a fluorescence parameters wi...

  16. Cutaneous absorption of Oleander: Fact or fiction.

    PubMed

    Senthilkumaran, S; Saravanakumar, S; Thirumalaikolundusubramanian, P

    2009-01-01

    Cardiac conduction disorders following oral ingestion of Oleander plant materials were documented earlier. Transcutaneous absorption of yellow oleander (Thevetia peruviana) leaf extract applied over non intact skin (raw wound) resulting in reversible cardiac conduction disorder observed in four healthy males who were free from any other systemic or electrolyte or metabolic disorders or exposure to pesticide or toxins is reported for the first time. Their hematological, biochemical, clinical, and echocardiogram status were within normal limits and free of any abnormalities. One among the four, presented for weakness and breathlessness (class II). He had bradycardia with Mobitz II block and hypotension without any other demonstrable localizing signs. The other three were identified in the community and without any symptoms. However, their ECG revealed bradycardia with Mobitz I block in two and complete heart block in the other. All of the four recovered well without any untoward events. Hence, it is suggested that physicians and practitioners have to elicit history and route of administration of unconventional therapy, whenever they are confronted with clinical challenges and during medical emergencies before embarking final decision. PMID:19561955

  17. Cutaneous absorption of Oleander: Fact or fiction

    PubMed Central

    Senthilkumaran, S; Saravanakumar, S; Thirumalaikolundusubramanian, P

    2009-01-01

    Cardiac conduction disorders following oral ingestion of Oleander plant materials were documented earlier. Transcutaneous absorption of yellow oleander (Thevetia peruviana) leaf extract applied over non intact skin (raw wound) resulting in reversible cardiac conduction disorder observed in four healthy males who were free from any other systemic or electrolyte or metabolic disorders or exposure to pesticide or toxins is reported for the first time. Their hematological, biochemical, clinical, and echocardiogram status were within normal limits and free of any abnormalities. One among the four, presented for weakness and breathlessness (class II). He had bradycardia with Mobitz II block and hypotension without any other demonstrable localizing signs. The other three were identified in the community and without any symptoms. However, their ECG revealed bradycardia with Mobitz I block in two and complete heart block in the other. All of the four recovered well without any untoward events. Hence, it is suggested that physicians and practitioners have to elicit history and route of administration of unconventional therapy, whenever they are confronted with clinical challenges and during medical emergencies before embarking final decision. PMID:19561955

  18. Specific detection and identification of Xylella fastidiosa strains causing oleander leaf scorch by polymerase chain reaction

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A pair of PCR primers, QH-OLS05/QH-OLS08, was developed that is specific for strains of Xylella fastidiosa causing oleander leaf scorch. The primers were designed based on DNA sequence of a randomly amplified polymorphic DNA (RAPD)-PCR product unique to oleander strains. The PCR assay using primer p...

  19. Pharmacological aspects of Nerium indicum Mill: A comprehensive review

    PubMed Central

    Dey, Priyankar; Chaudhuri, Tapas Kumar

    2014-01-01

    Phytomedicine is the oldest medical practice known to man. Since the dawn of mankind, various plant resources are used to cure different diseases and also for a long and healthy life. The ancient knowledge of plant based medicine has transferred from generations to generations and accumulated as ethnopharmacological knowledge among different ethnic groups. India is the spanning bed of traditional phytomedicinal system where Ayurveda was born out of the knowledge of traditional medicine. In various other countries of South-Eastern Asia, South America, and in Arabian countries, still today, a great number of people rely primarily on phytomedicines to cure diseases. In the complementary and alternative medicinal systems, Nerium indicum is one such plant which is famed for its therapeutic efficiency in different diseases globally. In the present time, when the pharmaceutical companies are concentrating more toward the plant based traditional medicines to avoid the side-effects and resistance against synthetic drugs, N. indicum has proved its efficiency in different disease models. Therefore, this review comprehensively covers the medicinal and pharmacological activities of different parts of the plant N. indicum. PMID:25125887

  20. A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A TaqMan-based real-time PCR assay is developed for strains of X. fastidiosa causing oleander leaf scorch. The assay uses primers WG-OLS-F1 and WG-OLS-R1 and the fluorescent probe WG-OLS-P1, designed based on unique sequences present only in genomic sequence of oleander strain Ann1. The assay is spe...

  1. The toxic activities of Arisaema erubescens and Nerium indicum mixed with Streptomycete against snails.

    PubMed

    Zhang, Yi; Ke, Wenshan; Yang, Jinglian; Ma, Anning; Yu, Zhensen

    2009-03-01

    The comparative molluscicidal activities of Arisaema erubescens tuber extracts and Nerium indicum leaf extracts mixed with Streptomycete violacerruber dilution (SD) against the snail Oncomlania hupensis and the responses of the isozymes, esterase (EST) and superoxide dismutase (SOD) to the A. erubescens extracts and the mixtures were investigated. The molluscicidal activity of A. erubescens water extracts mixed with S. violacerruber dilution was 4-5 times higher than a single A. erubescens or S. violacerruber dilution after 24-h exposure, and is also higher than that of N. indicum leaf water extracts mixed with S. violacerruber dilution. At the end of exposure to the N-butanol extracts of A. erubescens tubers (NEAT), the EST activity in snail liver decreased and some enzyme bands (EST 1 and EST 3 in exposure to NEAT) disappeared but the activities of SOD 1 increased. The effect was more obvious in mixture treatment than in single NEAT or SD treatment. The results indicated that molluscicidal activities of plant and microorganism could be more effective than single plant. The decline of the detoxic ability in snail liver cells could be the reason of the snail dying. PMID:21783953

  2. A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander.

    PubMed

    Guan, Wei; Shao, Jonathan; Singh, Raghuwinder; Davis, Robert E; Zhao, Tingchang; Huang, Qi

    2013-02-15

    A TaqMan-based real-time PCR assay was developed for specific detection of strains of X. fastidiosa causing oleander leaf scorch. The assay uses primers WG-OLS-F1 and WG-OLS-R1 and the fluorescent probe WG-OLS-P1, designed based on unique sequences found only in the genome of oleander strain Ann1. The assay is specific, allowing detection of only oleander-infecting strains, not other strains of X. fastidiosa nor other plant-associated bacteria tested. The assay is also sensitive, with a detection limit of 10.4fg DNA of X. fastidiosa per reaction in vitro and in planta. The assay can also be applied to detect low numbers of X. fastidiosa in insect samples, or further developed into a multiplex real-time PCR assay to simultaneously detect and distinguish diverse strains of X. fastidiosa that may occupy the same hosts or insect vectors. Specific and sensitive detection and quantification of oleander strains of X. fastidiosa should be useful for disease diagnosis, epidemiological studies, management of oleander leaf scorch disease, and resistance screening for oleander shrubs. PMID:23165115

  3. Oleander poisoning

    MedlinePLUS

    ... Elsevier; 2011:chap 64. Hostetler M, Schreiber S. Poisonous plants. In: Tintinalli JE, Kelen GD, Stapczynski JS, Ma OJ, Cline DM, eds. Emergency Medicine: A Comprehensive Study Guide . 6th ed. New York, NY: McGraw-Hill; ...

  4. A regional analysis of weather mediated competition between a parasitoid and a coccinellid predator of oleander scale.

    PubMed

    Gutierrez, Andrew P; Pizzamiglio, Marina A

    2007-01-01

    The regulation of an asexual population of the oleander scale [Aspidiotus nerii Bouch (Hemiptera: Diaspididae)] on California bay tree [Umbellularia californica (Hopk. & Arn.) Nut.] by two natural enemies; an idiobiont, ectoparasitoid Aphytis chilensis Howard (Hymenoptera: Aphelinidae) and a coccinellid predator (Rhysobius lophanthae (Blaisd.) (Coleoptera: Coccinellidae), was examined using a general weather-driven, tri-trophic, physiologically based age-mass structured demographic model. The model is of intermediate complexity and was parameterized using extensive laboratory data and field observations from Albany, CA. Temperature-dependent physiological indices were estimated from the laboratory data and used to scale per capita growth, fecundity and survivorship rates from maximal values in a time varying environment. The tri-trophic model was integrated in a GIS (geographic information system) and the species dynamics examined across years and across the ecological zones of California. Field data and simulation results suggested the coccinellid predator was the most important regulating agent of oleander scale in the mild climate of Albany. However, multiple linear regression analysis of simulation data across all ecological zones of California shows that the parasitoid A. chilensis is the most important agent in suppressing oleander scale densities in warmer climates, while the predator R. lophanthae increases scale density an average of 9.7% across all regions. PMID:17420864

  5. Effect of various growth hormone concentration and combination on callus induction, nature of callus and callogenic response of Nerium odorum.

    PubMed

    Rashmi, Runa; Trivedi, Maheshwar Prasad

    2014-03-01

    Nerium odorum, Linn. (Apocynaceae) is an important evergreen shrub. It is heat, salinity and drought tolerant. Plants with milky sap have medicinal value, mainly cardenolides, flavonoids and terpenes. It is used for wastewater purification and for restoration of riparian woodlands. In view of these facts, the study was conducted for micropropagation of N. odorum. Murashige and Skoog (MS) media supplemented with different concentrations (0.5-10.0 mg/l) of 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and kinetin (Kin) were used singly and in combinations. Among all the growth hormones, 2,4-D was the best for callus induction (75% in stem and 79% in leaf) and in combination 2,4-D and BAP (78% in stem and 81% in leaf). The day of callus induction started from the 19th to the 37th day. This variation is due to the differences in culture conditions and the age of explants. The fresh and dry weight and moisture content showed good growth of callus, which is used in further studies of alkaloid production. Micropropagation of this plant allows the production of clones at a fast rate and in continuous manner. This work can lead to the development of an efficient protocol for callus induction and other issues. PMID:24407943

  6. Molluscicidal activity of cardiac glycosides from Nerium indicum against Pomacea canaliculata and its implications for the mechanisms of toxicity.

    PubMed

    Dai, Lingpeng; Wang, Wanxian; Dong, Xinjiao; Hu, Renyong; Nan, Xuyang

    2011-09-01

    Cardiac glycosides from fresh leaves of Nerium indicum were evaluated for its molluscicidal activity against Pomacea canaliculata (golden apple snail: GAS) under laboratory conditions. The results showed that LC(50) value of cardiac glycosides against GAS was time dependent and the LC(50) value at 96 h was as low as 3.71 mg/L, which was comparable with that of metaldehyde at 72 h (3.88 mg/L). These results indicate that cardiac glycosides could be an effective molluscicide against GAS. The toxicological mechanism of cardiac glucosides on GAS was also evaluated through changes of selected biochemical parameters, including cholinesterase (ChE) and esterase (EST) activities, glycogen and protein contents in hepatopancreas tissues of GAS. Exposure to sublethal concentrations of cardiac glycosides, GAS showed lower activities of EST isozyme in the later stages of the exposure period as well as drastically decreased glycogen content, although total protein content was not affected at the end of 24 and 48 h followed by a significant depletion at the end of 72 and 96 h. The initial increase followed by a decline of ChE activity was also observed during the experiment. These results suggest that cardiac glycosides seriously impair normal physiological metabolism, resulting in fatal alterations in major biochemical constituents of hepatopancreas tissues of P. canaliculata. PMID:21843803

  7. The Oleander Program - 9 years of Gulf Stream Sampling and Still Going Strong!

    NASA Astrophysics Data System (ADS)

    Rossby, T.

    2001-12-01

    Starting in Fall 1992 we have been monitoring the currents between the mid-Atlantic Bight and the NW Sargasso Sea with an acoustic Doppler current profiler on the freighter CMV Oleander, which makes weekly roundtrips between Port Elizabeth, NJ and Bermuda. In addition, XBTs and surface salts have been taken on a monthly basis since 1979. These systematic observations of the upper ocean are giving us new insights into the structure of the Gulf Stream and adjacent waters. In this overview we will highlight some of the major findings of this ongoing program. One of the more striking observations is perhaps the structural stability of the Gulf Stream itself. Its shape can be characterized as a double-exponential which results from the mixing or homogenization of waters between the current and either side, but not across it. We show that 80 percent of the Eulerian eddy kinetic energy that is observed in the Gulf Stream can be described in terms of the meandering of a rigid double-exponential current. The remaining variability can be accounted for in terms of a few structural modes that are most likely associated with the meandering of the current. We have found that the transport of the current has been conspicuously stable, and will argue that past thoughts about large variations in transport may result from an inability to distinguish between the current itself and adjacent local recirculations of varying intensity. The distinction is made clear thanks to the repeat sampling. However, the Gulf Stream does exhibit significant variations in mean path on interannual time scales. These show a strong correlation with temperature-salinity anomalies in the Slope Sea. We suggest that both result from time-varying transports from the Labrador shelf, but there is presently considerable discussion as to whether the path shifting should be viewed as a thermohaline or a winddriven process. More generally, we use the above examples to argue that with more deliberate planning, the unparalleled and repeat access to in-situ sampling of the oceans provided by commercial shipping and cruise vessels could provide society with far more extensive and valuable information about the ocean and atmospheric conditions at sea. But for this to happen, the instrumentation needs to be optimized for completely automatic and unattended operation. This also means working with the merchant marine community to develop guidelines and procedures for future cooperative efforts.

  8. Sex pheromone of the oleander scale, Aspidiotus nerii: structural characterization and absolute configuration of an unusual functionalized cyclobutane.

    PubMed

    Einhorn, J; Guerrero, A; Ducrot, P H; Boyer, F D; Gieselmann, M; Roelofs, W

    1998-08-18

    The sex pheromone emitted by the female oleander scale, Aspidiotus nerii (Homoptera, Diaspididae), has been isolated and characterized as (1R, 2S)-cis-2-isopropenyl-1-(4'-methyl-4'-penten-1'-yl)cyclobutaneethanol acetate by using advanced MS and NMR spectroscopic methods, as well as a variety of microderivatization sequences. The structure has been confirmed by stereo- and enantioselective synthesis of the four possible stereoisomers. The absolute configuration has been determined by comparison of the activity of the cis (1S,2R) and (1R, 2S) enantiomers with that exhibited by the natural material in greenhouse bioassays and field tests. The structure of this sesquiterpenoid pheromone is new in the coccids and in the pheromone field in general. PMID:9707567

  9. Sex pheromone of the oleander scale, Aspidiotus nerii: Structural characterization and absolute configuration of an unusual functionalized cyclobutane

    PubMed Central

    Einhorn, Jacques; Guerrero, Angel; Ducrot, Paul-Henri; Boyer, Franois-Didier; Gieselmann, Mary; Roelofs, Wendell

    1998-01-01

    The sex pheromone emitted by the female oleander scale, Aspidiotus nerii (Homoptera, Diaspididae), has been isolated and characterized as (1R,2S)-cis-2-isopropenyl-1-(4?-methyl-4?-penten-1?-yl)cyclobutaneethanol acetate by using advanced MS and NMR spectroscopic methods, as well as a variety of microderivatization sequences. The structure has been confirmed by stereo- and enantioselective synthesis of the four possible stereoisomers. The absolute configuration has been determined by comparison of the activity of the cis (1S,2R) and (1R,2S) enantiomers with that exhibited by the natural material in greenhouse bioassays and field tests. The structure of this sesquiterpenoid pheromone is new in the coccids and in the pheromone field in general. PMID:9707567

  10. Recent accelerated warming of the continental shelf off New Jersey: Observations from the CMV Oleander expendable bathythermograph line

    NASA Astrophysics Data System (ADS)

    Forsyth, Jacob Samuel Tse; Andres, Magdalena; Gawarkiewicz, Glen G.

    2015-03-01

    Expendable bathythermographs (XBTs) have been launched along a repeat track from New Jersey to Bermuda from the CMV Oleander through the NOAA/NEFSC Ship of Opportunity Program about 14 times per year since 1977. The XBT temperatures on the Middle Atlantic Bight shelf are binned with 10 km horizontal and 5 m vertical resolution to produce monthly, seasonally, and annually averaged cross-shelf temperature sections. The depth-averaged shelf temperature, Ts, calculated from annually averaged sections that are spatially averaged across the shelf, increases at 0.026 ± 0.001°C yr-1 from 1977 to 2013, with the recent trend substantially larger than the overall 37 year trend (0.11 ± 0.02°C yr-1 since 2002). The Oleander temperature sections suggest that the recent acceleration in warming on the shelf is not confined to the surface, but occurs throughout the water column with some contribution from interactions between the shelf and the adjacent Slope Sea reflected in cross-shelf motions of the shelfbreak front. The local warming on the shelf cannot explain the region's amplified rate of sea level rise relative to the global mean. Additionally, Ts exhibits significant interannual variability with the warmest anomalies increasing in intensity over the 37 year record even as the cold anomalies remain relatively uniform throughout the record. Ts anomalies are not correlated with annually averaged coastal sea level anomalies at zero lag. However, positive correlation is found between 2 year lagged Ts anomalies and coastal sea level anomalies, suggesting that the region's sea level anomalies may serve as a predictor of shelf temperature.

  11. Multilocus sequence typing of Xylella fastidiosa causing Pierce's disease and oleander leaf scorch in the United States.

    PubMed

    Yuan, Xiaoli; Morano, Lisa; Bromley, Robin; Spring-Pearson, Senanu; Stouthamer, Richard; Nunney, Leonard

    2010-06-01

    Using a modified multilocus sequence typing (MLST) scheme for the bacterial plant pathogen Xylella fastidiosa based on the same seven housekeeping genes employed in a previously published MLST, we studied the genetic diversity of two subspecies, X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. sandyi, which cause Pierce's disease and oleander leaf scorch, respectively. Typing of 85 U.S. isolates (plus one from northern Mexico) of X. fastidiosa subsp. fastidiosa from 15 different plant hosts and 21 isolates of X. fastidiosa subsp. sandyi from 4 different hosts in California and Texas supported their subspecific status. Analysis using the MLST genes plus one cell-surface gene showed no significant genetic differentiation based on geography or host plant within either subspecies. Two cases of homologous recombination (with X. fastidiosa subsp. multiplex, the third U.S. subspecies) were detected in X. fastidiosa subsp. fastidiosa. Excluding recombination, MLST site polymorphism in X. fastidiosa subsp. fastidiosa (0.048%) and X. fastidiosa subsp. sandyi (0.000%) was substantially lower than in X. fastidiosa subsp. multiplex (0.240%), consistent with the hypothesis that X. fastidiosa subspp. fastidiosa and sandyi were introduced into the United States (probably just prior to 1880 and 1980, respectively). Using whole-genome analysis, we showed that MLST is more effective at genetic discrimination at the specific and subspecific level than other typing methods applied to X. fastidiosa. Moreover, MLST is the only technique effective in detecting recombination. PMID:20465416

  12. Interannual to decadal temperature variability in the north-west Atlantic: Observations from the MV Oleander XBT line

    NASA Astrophysics Data System (ADS)

    Forsyth, J. S. T.; Andres, M.; Gawarkiwicz, G.

    2014-12-01

    Despite convincing evidence of deep ocean warming, temperature changes over the shelves have proven difficult to quantify as most long-term records lack the spatial and temporal resolution needed to resolve shelf variability. XBT data have been collected for 37 years along a repeat track from New Jersey to Bermuda from the MV Oleander providing the resolution necessary for shelf analysis. The XBT temperature data on the shelf (onshore of the 80 m isobath) were binned with 10 km horizontal and 5 m vertical resolution to produce monthly and annually averaged temperature sections. A climatology produced from the binned data identifies key seasonal temperature features consistent with previous climatologies, showing the utility of the XBT data. Annual spatially-averaged shelf temperatures have trended upwards since the beginning of the record in 1977 (0.025 C/yr), with recent trends (i.e., since 2002, 0.10 C/yr) substantially larger than the overall 37- year trend. Comparison of composite sections for the most anomalous years suggests that the interannual variability in the spatially-averaged temperatures is most heavily influenced by temperature anomalies near the shelf break. The spatially-averaged temperature anomalies are not correlated with annually-averaged coastal sea level anomalies from tide gauges at zero lag, which suggest that interannual variability in coastal sea level is not due to thermo steric effects. However, a strong positive correlation is found between 2-year lagged temperature anomalies and coastal sea level anomalies. This relationship is most pronounced for the shelf break temperature anomalies, with the strongest 2-year lag correlations found in winter and spring. Connections between the observed interannual to decadal temperature variability on the shelf and variability in the AMOC are being investigated in an ongoing effort to better understand open-ocean/shelf interactions in the Northwest Atlantic.

  13. Epidemiology of intoxication of domestic animals by plants in Europe.

    PubMed

    Cortinovis, Cristina; Caloni, Francesca

    2013-08-01

    This review focuses on some of the most important poisonous plants in Europe and provides an overview of the poisoning episodes that have occurred in European countries. Poisoning of livestock and companion animals by plants is a relatively common occurrence. In Europe livestock and horses are commonly poisoned by Datura stramonium (Jimson weed), Senecio spp. (ragworts and groundsels), Quercus spp. (oak), Taxus baccata (European yew), Nerium oleander (oleander), Pteridium aquilinum (bracken fern), Robinia pseudoacacia (black locust) and Rhododendron spp. (rhododendrons and azaleas). Poisoning may occur when the fresh plant is ingested in pasture or when it contaminates hay or silage. In pets, the greatest majority of plant poisonings are the result of ingestion of house or garden plants, such as Cycas revoluta (Sago palm), Ricinus communis (castor bean), Allium spp., Euphorbia pulcherrima (poinsettia), Lilium spp., Convallaria majalis (Lily of the valley), Pyracantha spp. (firethorn), Rhododendron spp. (rhododendrons and azaleas), Melia azedarach (Chinaberry tree), Taxus baccata (European yew) and Nerium oleander (oleander). PMID:23570777

  14. From the CMV Oleander Project: A Study of the Shelfbreak Front of the Middle Atlantic Bight From Long-term ADCP and Hydrographic Data

    NASA Astrophysics Data System (ADS)

    Flagg, C. N.; Dunn, M.; Wang, D.

    2004-12-01

    Utilizing the first decade of shipboard ADCP data as well as XBT and surface salinity data obtained from the CMV Oleander, this study is focused on the mean structure, and seasonal and interannual variability of the frontal zone at the edge of the shelf of the Middle Atlantic Bight. The early analysis showed that more than half the data in the frontal zone were influenced by warm core rings and that removing the confounding influence of the rings was vital if the true structure of the front was to emerge. From the culled data set of 128 transects of the front with sufficient coverage we have proceeded to generate a velocity description following the core of the frontal jet showing a maximum, surface intensified velocity of more than 0.25 ms-1, a vertical extent of roughly 80 m, a half-amplitude width of about 20 km and an alongshore transport of ~0.34 Sv. The maximum mean relative vorticity of the jet is 0.56*f. The alongshore jet is accompanied by a substantial surface intensified convergent flow that implies a maximum down-welling in the center of the jet of ~30 m/day. The seasonally the shelfbreak jet has its minimal velocities during the summer months, increasing to maximal velocities during the winter before decreasing agin in the spring. An interesting feature that emerges from the ADCP data is that while the shelfbreak frontal jet is usually assumed to consist of a single high-speed core, in fact, the jet often exhibits multiple high velocity extrema, the existence of which appears to undergo a seasonal progression.

  15. Thermal emissivity of leaves from trees cultivated using processed wastewater

    SciTech Connect

    Drakatos, P.A.; Kalavrouziotis, I.K.; Skuras, D.G.; Drakatos, S.P.

    1997-07-01

    Wastewater and sludge from wastewater treatment plans were discharged on experimental plantations of the species Nerium oleander, Eucalyptus sp. and Populus tremula. An emissiometer was used to measure the thermal emissitivity of the leaves of the different species. Comparison of thermal emissitivity between control and treatment leaves showed significant differences. There are clear indications that, land disposal of wastewater and sludge affects emissivity of different plant-species.

  16. Flammability of some ornamental species in wildland-urban interfaces in southeastern France: laboratory assessment at particle level.

    PubMed

    Ganteaume, Anne; Jappiot, Marielle; Lampin, Corinne; Guijarro, Mercedes; Hernando, Carmen

    2013-08-01

    Assessment of the flammability of ornamental vegetation (particularly hedges) planted around houses is necessary in light of the increasing urbanization of the wildland-urban interfaces (WUIs) and the high fire occurrence in such areas. The structure and flammability of seven of the species most frequently planted as hedges in Provence (southeastern France) were studied at particle level. Spatial repartition of the different types of fuel particles within plants was assessed by means of the cube method. The leaf flammability was assessed using an epiradiator as a burning device, and measurements of foliar physical characteristics and gross heat of combustion (GHC) helped to explain the results of burning experiments. Co-inertia analysis revealed that species with thin leaves were quick to ignite (Pyracantha coccinea, Phyllostachys sp.) and species with high leaf GHC burned the longest (Pittosporum tobira, Nerium oleander). Species presenting high ignitability (Photinia fraseri, Phyllostachys sp. and Pyracantha coccinea) were characterized by high foliar surface area-to-volume ratio, and species presenting lower ignitability were characterized by high GHC (Pittosporum tobira, Nerium oleander, Cupressus sempervirens). Hierarchical cluster analysis of the flammability variables (ignition frequency, time-to-ignition and flaming duration) categorized the relative flammability of the seven species (including dead Cupressus sempervirens) in five clusters of species from poorly flammable (Pittosporum tobira) to extremely flammable (dead Cupressus sempervirens).This study provides useful information for reducing fire risk in WUIs in the study area. PMID:23765042

  17. Selection of woody species for wastewater enhancement and restoration of riparian woodlands.

    PubMed

    Adrover, M; Forss, A L; Ramon, G; Vadell, J; Moya, G; Taberner, A Martinez

    2008-05-01

    Growth and nutrient uptake of seven tree species were evaluated with the goal of selecting the species that can be used for wastewater enhancement by dendro-purification, or green tree filtering, and for restoration of riparian woodlands. Trees were grown in pots with an inert mixture of perlite and vermiculite and irrigated with either nutrient solution or treated wastewater We measured the effects of species and irrigation water on biomass and nutrient content of leaves, stems and roots. For most of the species, treated wastewater had a positive effect on final biomass and above ground: below ground ratio compared to that of nutrient solution. However, growth of Cupressus sempervirens and Populus nigra were inhibited by water sodium concentration. Nerium oleander, Tamarix africana and Vitex agnus-castus were the species with the greatest final biomass. Pistacia terebinthus had the highest nitrogen and phosphorus content in leaves, stems and roots, while N. oleander and V. agnus-castus showed the best potassium accumulation. In general, P. terebinthus, N. oleander, T. africana and V. agnus-castus were the best qualified species for purification of wastewater. PMID:18972692

  18. Comparison of Water Potentials Measured by In Situ Psychrometry and Pressure Chamber in Morphologically Different Species 1

    PubMed Central

    Turner, Neil C.; Spurway, R. A.; Schulze, E.-D.

    1984-01-01

    Leaf water potentials measured by in situ psychrometry were compared with leaf water potentials measured by the pressure chamber technique at various values of water potential in Helianthus annuus, Helianthus nuttallii, Vigna unguiculata, Nerium oleander, Pistacia vera, and Corylus avellana. In V. unguiculata, the leaf water potentials measured by the in situ psychrometer oscillated at the same periodicity as, and proportional to, the leaf conductance. In all species, potentials measured by in situ psychrometers operating in the psychrometric mode were linearly correlated with potentials measured with the pressure chamber. However, the in situ psychrometers underestimated the leaf water potential in the two Helianthus species at low water potentials and overestimated the water potential in P. vera, N. oleander, and C. avellana. The underestimation in the two Helianthus species at low water potentials resulted from differences in water potential across the leaf. The overestimation in P. vera, N. oleander, and C. avellana was considered to arise from low epidermal conductances in these species even after abrasion of the cuticle. Pressure-volume studies with Lycopersicon esculentum showed that less water was expressed from distal than proximal leaflets when the whole leaf was slowly pressurized. The implication of this for water relations characteristics obtained by pressure-volume techniques is discussed. We conclude that in situ psychrometers are suitable for following dynamic changes in leaf water potential, but should be used with caution on leaves with low epidermal conductances. PMID:16663415

  19. Les Brulures Chimiques Par Le Laurier Rose

    PubMed Central

    Bakkali, H.; Ababou, M.; Nassim Sabah, T.; Moussaoui, A.; Ennouhi, A.; Fouadi, F.Z.; Siah, S.; Ihrai, H.

    2010-01-01

    Summary Le laurier rose ou Nerium oleander est un arbuste qui pousse naturellement dans les régions méditerranéennes. Au Maroc on le trouve dans les lieux humides. Il est réputé par ses risques de toxicité systémique en cas d'empoisonnement à cause de la présence de deux alcaloïdes, surtout l'oléandrine. La littérature illustre des cas d'utilisation locale des feuilles de cette plante contre la gale, les hémorroïdes et les furoncles. Nous rapportons deux cas de brûlures chimiques par le laurier rose de gravité différente. Cela doit aboutir à une information élargie de la population, ainsi qu'une réglementation stricte de sa commercialisation. PMID:21991211

  20. caros del mango

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Los caros constituyen un grupo abundante y diverso que ocupa diferentes hbitats en rboles frutales y la estructura y disposicin del follaje y ramas del mango, contribuyen significativamente a que se presente gran diversidad de caros benficos y dainos asociados a esta especie frutal. En Colomb...

  1. Preguntas y respuestas acerca del Estudio del

    Cancer.gov

    El Estudio del Tamoxifeno y Raloxifeno (STAR, por sus siglas en ingls) es un estudio clnico (un estudio de investigacin conducido con voluntarios) diseado para ver cómo el medicamento raloxifeno (Evista) se compara con el medicamento tamoxifeno (Nolvadex)

  2. Acute death associated with Citrobacter freundii infection in an African elephant (Loxodonta africana).

    PubMed

    Ortega, Joaqun; Corpa, Juan M; Orden, Jos A; Blanco, Jorge; Carbonell, Mara D; Gerique, Amalia C; Latimer, Erin; Hayward, Gary S; Roemmelt, Andreas; Kraemer, Thomas; Romey, Aurore; Kassimi, Labib B; Casares, Miguel

    2015-09-01

    A 21-year-old male African elephant (Loxodonta africana) died suddenly with no previous medical history. Grossly, there were severe multifocal epicardial and endocardial hemorrhages of the atria and ventricles, hydropericardium, multifocal pleural hemorrhages, and severe pulmonary congestion and edema. Histologically, there was fibrinoid vasculitis and thrombosis in the heart and lung and myocardial necrosis. Citrobacter freundii was isolated in abundance in pure culture from liver and heart samples. Low levels of multiples types of elephant endotheliotropic herpesvirus (EEHV-6, EEHV-2B, and EEHV-3A) were detected in spleen samples, but not in heart samples. The levels of EEHV DNA found were much lower than those usually associated with acute EEHV hemorrhagic disease, and many other genomic loci that would normally be found in such cases were evidently below the level of detection. Therefore, these findings are unlikely to indicate lethal EEHV disease. Polymerase chain reaction for encephalomyocarditis virus (EMCV) and toxicology for oleander (Nerium oleander) were negative. Stress, resulting from recent transport, and antimicrobial therapy may have contributed to the death of this animal. PMID:26179092

  3. Do cancer cells in human and meristematic cells in plant exhibit similar responses toward plant extracts with cytotoxic activities?

    PubMed

    Khalifa, Noha S; Barakat, Hoda S; Elhallouty, Salwa; Salem, Dina

    2015-01-01

    We examined the effect of water extracts of Persea americana fruit, and of the leaves of Tabernamontana divericata, Nerium oleander and Annona cherimolia (positive control) on Vicia faba root cells. We had confirmed in our previously published data the cytotoxicity of these plant extracts on four human cancer cell lines: liver (HepG-2), lung (A549), colon (HT-29) and breast (MCF-7). Vicia faba roots were soaked in plant extracts at dilutions of 100, 1,250, 2,500, 5,000, 10,000, 20,000 ppm for 4 and 24 h. All treatments resulted in a significant reduction in the mitotic index in a dose dependant manner. Root cells treated with T. divericata, N. oleander and A. cherimolia exhibited a decrease in prophase cell percentage, increase in micronuclei and chromosomal abnormalities as concentration increased. The P. americana treatment showed the highest cytotoxic effect on cancer cells, prophase cell percentage increased linearly with the applied concentration and no micronuclei were detected. This study shows that root tip assay of beans can be used in initial screening for new plant extracts to validate their use as candidates for containing active cytotoxic agents against malignant cells. This will greatly help in exploring new plant extracts as drugs for cancer treatment. PMID:24705601

  4. Instantnea del cncer de prstata

    Cancer.gov

    Informacin sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cncer de prstata; as como ejemplos de actividades del NCI y adelantos en la investigacin de este tipo de cncer.

  5. Instantnea del cncer de rin

    Cancer.gov

    Informacin sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cncer de rin; as como ejemplos de actividades del NCI y adelantos en la investigacin de este tipo de cncer.

  6. Hojas informativas del NCI

    Cancer.gov

    La colección de hojas informativas del NCI trata de una variedad de temas relacionados con el cáncer. Las hojas informativas se revisan y ponen al día de acuerdo a las investigaciones más recientes sobre el cáncer.

  7. Estudio del CH interestelar

    NASA Astrophysics Data System (ADS)

    Olano, C.; Lemarchand, G.; Sanz, A. J.; Bava, J. A.

    El objetivo principal de este proyecto consiste en el estudio de la distribución y abundancia del CH en nubes interestelares a través de la observación de las líneas hiperfinas del CH en 3,3 GHz. El CH es una molécula de amplia distribución en el espacio interestelar y una de las pocas especies que han sido observadas tanto con técnicas de radio como ópticas. Desde el punto de vista tecnológico se ha desarrollado un cabezal de receptor que permitirá la realización de observaciones polarimétricas en la frecuencia de 3,3 GHz, con una temperatura del sistema de 60 K y un ancho de banda de 140 MHz, y que será instalado en el foco primario de la antena parabólica del IAR. El cabezal del receptor es capaz de detectar señales polarizadas, separando las componentes de polarización circular derecha e izquierda. Para tal fin el cabezal consta de dos ramas receptoras que amplificarán la señal y la trasladarán a una frecuencia más baja (frecuencia intermedia), permitiendo de esa forma un mejor transporte de la señal a la sala de control para su posterior procesamiento. El receptor además de tener características polarimétricas, podrá ser usado en el continuo y en la línea, utilizando las ventajas observacionales y de procesamiento de señal que actualmente posee el IAR.

  8. Nevado del Huila, Columbia

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Nevado del Huila Volcano in Colombia is actually a volcanic chain running north to south, capped by a glacier. With peaks ranging in height from 2,600 to 5,780 meters (8,530 to 18,960 feet), Nevado del Huila is a stratovolcano composed of alternating layers of hardened lava, solidified ash, and volcanic rocks. Its first recorded eruption occurred in the mid-sixteenth century. The long-dormant volcano erupted again in mid-April 2007. A few months before the eruption, the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) on NASA's Terra satellite captured this image of Nevado del Huila, on February 23, 2007. In this image, the bright white area just east of the central summit is ice. Immediately west of the summit are bare rocks, appearing as blue-gray. West of those rocks, white reappears, but this patch of white results from clouds hovering in the nearby valley. In the east, the colors turn to brown (indicating bare rock) and bright green (indicating vegetation). ASTER photographed Nevado del Huila near the end of a long phase of quietude. On April 17, 2007, local authorities recorded seismic activity associated with rock fracturing on the volcano's central summit, according to the ReliefWeb Website. Activity intensified the following day with an eruption and mudflows, forcing thousands of nearby residents to evacuate. As the Associated Press reported, the eruption caused avalanches and floods that wiped away both houses and bridges. It marked the volcano's first recorded eruption since the Spanish colonized the area five centuries earlier. NASA image created by Jesse Allen, using data provided courtesy of the NASA/GSFC/MITI/ERSDAC/JAROS, and U.S./Japan ASTER Science Team.

  9. Atlas del Genoma del Cáncer: Antecedentes

    Cancer.gov

    El Atlas del Genoma del Cáncer es una iniciativa de los Institutos Nacionales de la Salud (NIH) para crear mapas multidimensionales completos de los cambios genómicos clave en los tipos y subtipos principales de cáncer.

  10. Case Study: del Amo Bioventing

    EPA Science Inventory

    The attached presentation discusses the fundamentals of bioventing in the vadose zone. The basics of bioventing are presented. The experience to date with the del Amo Superfund Site is presented as a case study.

  11. Infografa: beneficios y perjuicios de la prueba del PSA para la deteccin del cncer de prstata

    Cancer.gov

    Infografa que muestra los beneficios y perjuicios de la prueba del PSA para la deteccin del cncer de prstata, segn estimaciones de dos estudios sobre exmenes de deteccin del cncer de prstata.

  12. Particle pollution - An environmental magnetism study using biocollectors located in northern Portugal

    NASA Astrophysics Data System (ADS)

    Sant'Ovaia, Helena; Lacerda, Maria João; Gomes, Celeste

    2012-12-01

    In this study the magnetic properties of tree leaves were measured in order to compare their capability to accumulate particles, to establish the relationship between magnetic properties and chemical data and to assess the particle pollution in selected locations in the cities of Braga, Porto, Valongo and Trancoso-Reboleiro, northern Portugal. In Porto, Braga and Valongo, leaves from the evergreen Nerium oleander were sampled each month during a year. N. oleander and deciduous Quercus spp. and Platanus spp. samples were collected in the same site in Porto, in order to determine the ability of these different leaves to accumulate particles. The leaves of deciduous Tilia spp. were collected in Porto and in a rural area (Trancoso-Reboleiro) so that a comparison could be established between them. The results indicated a contrast between the urban and the rural areas. The highest concentration of magnetic particles was found in the sampling site of Valongo and the lowest concentration in the sampling site of Trancoso-Reboleiro. In Porto, the results have shown that the Quercus leaves possessed the highest capability to accumulate particles even though it is a deciduous species. The IRM acquisition curves and the S-300 ratios found in the samples of the urban areas indicated the presence of magnetite-like structures. SIRM/χ ratio revealed particles whose dimensions ranged between 5 μm and 8 μm in urban areas. The chemical elements copper and iron have a significant positive correlation with χ and SIRM, which highlights the use of magnetic properties as a proxy for the concentration of these metals in atmospheric dust. The magnetic properties were interpreted taking into consideration the rainfall peaks and then compared with the PM10 concentration levels monitored in an air quality station in Porto. Our data corroborated that magnetic properties provide a fast and inexpensive tool to evaluate long-term urban pollution from anthropogenic origin, especially heavy traffic.

  13. Vacunas contra los virus del papiloma humano

    Cancer.gov

    Una hoja informativa acerca de las vacunas contra los virus del papiloma humano (VPH) para prevenir infecciones con ciertos tipos de VPH, los cuales son la causa principal del cáncer de cuello del útero o cérvix.

  14. Mal del pinto in Mexico*

    PubMed Central

    Marquez, Francisco; Rein, Charles R.; Arias, Oswaldo

    1955-01-01

    This report deals with the geographical distribution, prevalence, epidemiology, etiology, serological, clinical, and histopathological features, and treatment of mal del pinto, or pinta, in Mexico. Repository penicillin preparations (PAM and Panbiotic) have been found highly effective in the treatment of this endemic, non-venereal treponematosis. ImagesFIG. 1FIG. 2FIG. 3FIG. 4FIG. 5FIG. 6FIG. 7FIG. 8 PMID:13260889

  15. Anti-leukemic activities of alcoholic extracts of two traditional Indian medicinal plants.

    PubMed

    Bhargava, Shipra; Malhotra, Hemant; Rathore, Om Singh; Malhotra, Bharti; Sharma, Pratibha; Batra, Amla; Sharma, Asha; Chiplunkar, Shubhada V

    2015-11-01

    The present work aimed to investigate the anticancer in vitro activity of two plants commonly used in traditional Indian medicine: Zingiber officinale Roscoe and Nerium oleander L. The extracts of these plants were tested in vitro on several human leukemic cell lines, K562, THP-1, MOLT-4 and Jurkat. Cell growth inhibition was observed for both plant extracts with 50% inhibitory concentration (IC50) values ranging between 1 and 28 ?g/mL using SRB (sulphorodamine B) and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assays. Enhanced cell growth inhibition was observed when the extracts were combined with imatinib. Exposed cells showed cell cycle arrest, DNA damage and cytochrome c release, indicating that the mechanism of cytotoxicity could be via mitochondrial mediated apoptotic pathways. Combination of the extracts of these plants with standard cancer treatment may be a way of enhancing responses. Clinical studies in patients with chronic myeloid leukemia are planned at our center. PMID:25772975

  16. Arthrobacter siccitolerans sp. nov., a highly desiccation-tolerant, xeroprotectant-producing strain isolated from dry soil

    PubMed Central

    SantaCruz-Calvo, L.; Gonzlez-Lpez, J.

    2013-01-01

    A novel desiccation-tolerant, xeroprotectant-producing bacterium, designated strain 4J27T, was isolated from a Nerium oleander rhizosphere subjected to seasonal drought in Granada, Spain. Phylogenetic analysis based on 16S rRNA gene sequencing placed the isolate within the genus Arthrobacter, its closest relative being Arthrobacter phenanthrenivorans Shep3 DSM 18606T, with which it showed 99.23?% 16S rRNA gene sequence similarity. DNADNA hybridization measurements showed less than 25?% relatedness between strain 4J27T and Arthrobacter phenanthrenivorans DSM 18606T. The DNA base composition of strain 4J27T was 65.3 mol%. The main fatty acids were anteiso C15?:?0, anteiso C17?:?0, C16?:?0 and iso C16?:?0 and the major menaquinone was MK-9 (H2). The peptidoglycan type was A3? with an l-Lysl-Serl-Thrl-Ala interpeptide bridge. The bacterium tested positive for catalase activity and negative for oxidase activity. Phylogenetic, chemotaxonomic and phenotypic analyses indicated that the desiccation-tolerant strain 4J27T represents a novel species within the genus Arthrobacter, for which the name Arthrobacter siccitolerans is proposed. The type strain is 4J27T (?=?CECT 8257T?=?LMG 27359T). PMID:23771623

  17. Ephedra alte (Joint Pine): An Invasive, Problematic Weedy Species in Forestry and Fruit Tree Orchards in Jordan

    PubMed Central

    Qasem, Jamal R.

    2012-01-01

    A field survey was carried out to record plant species climbed by Ephedra alte in certain parts of Jordan during 20082010. Forty species of shrubs, ornamental, fruit, and forest trees belonging to 24 plant families suffered from the climbing habit of E. alte. Growth of host plants was adversely affected by E. alte growth that extended over their vegetation. In addition to its possible competition for water and nutrients, the extensive growth it forms over host species prevents photosynthesis, smothers growth and makes plants die underneath the extensive cover. However, E. alte did not climb all plant species, indicating a host preference range. Damaged fruit trees included Amygdalus communis, Citrus aurantifolia, Ficus carica, Olea europaea, Opuntia ficus-indica, and Punica granatum. Forestry species that were adversely affected included Acacia cyanophylla, Ceratonia siliqua, Crataegus azarolus, Cupressus sempervirens, Pinus halepensis, Pistacia atlantica, Pistacia palaestina, Quercus coccifera, Quercus infectoria, Retama raetam, Rhamnus palaestina, Rhus tripartita, and Zizyphus spina-christi. Woody ornamentals attacked were Ailanthus altissima, Hedera helix, Jasminum fruticans, Jasminum grandiflorum, Nerium oleander, and Pyracantha coccinea. Results indicated that E. alte is a strong competitive for light and can completely smother plants supporting its growth. A. communis, F. carica, R. palaestina, and C. azarolus were most frequently attacked. PMID:22645486

  18. Zeaxanthin and the Induction and Relaxation Kinetics of the Dissipation of Excess Excitation Energy in Leaves in 2% O2, 0% CO21

    PubMed Central

    Demmig-Adams, Barbara; Winter, Klaus; Krger, Almuth; Czygan, Franz-Christian

    1989-01-01

    The relationship between the carotenoid zeaxanthin, formed by violaxanthin de-epoxidation, and nonphotochemical fluorescence quenching (qNP) in the light was investigated in leaves of Glycine max during a transient from dark to light in 2% O2, 0% CO2 at 100 to 200 micromoles of photons per square meter per second. (a) Up to a qNP (which can vary between 0 and 1) of about 0.7, the zeaxanthin content of leaves was linearly correlated with qNP as well as with the rate constant for radiationless energy dissipation in the antenna chlorophyll (kD). Beyond this point, at very high degrees of fluorescence quenching, only kD was directly proportional to the zeaxanthin content. (b) The relationship between zeaxanthin and kD was quantitatively similar for the rapidly relaxing quenching induced in 2% O2, 0% CO2 at 200 micromoles of photons per square meter per second and for the sustained quenching induced by long-term exposure of Nerium oleander to drought in high light (B Demmig, K Winter, A Krger, F-C Czygan [1988] Plant Physiol 87: 17-24). These findings suggest that the same dissipation process may be induced by very different treatments and that this particular dissipation process can have widely different relaxation kinetics. (c) A rapid induction of strong nonphotochemical fluorescence quenching within about 1 minute was observed exclusively in leaves which already contained a background level of zeaxanthin. PMID:16666893

  19. In vitro antifugal activity of medicinal plant extract against Fusarium oxysporum f. sp. lycopersici race 3 the causal agent of tomato wilt.

    PubMed

    Isaac, G S; Abu-Tahon, M A

    2014-03-01

    Medicinal plant extracts of five plants; Adhatoda vasica, Eucalyptus globulus, Lantana camara, Nerium oleander and Ocimum basilicum collected from Cairo, Egypt were evaluated against Fusarium oxysporum f. sp. lycopersici race 3 in vitro conditions using water and certain organic solvents. The results revealed that cold distilled water extracts of O. basilicum and E. globulus were the most effective ones for inhibiting the growth of F. oxysporum f. sp. lycopersici. Butanolic and ethanolic extracts of the tested plants inhibited the pathogen growth to a higher extent than water extracts. Butanolic extract of O. basilicum completely inhibited the growth of F. oxysporum f. sp. lycopersici at concentrations 1.5 and 2.0% (v/v). Butanolic extracts (2.0%) of tested plants had a strong inhibitory effect on hydrolytic enzymes; β-glucosidase, pectin lyase and protease of F. oxysporum f. sp. lycopersici. This study has confirmed that the application of plant extracts, especially from O. basilicum for controlling F. oxysporum f. sp. lycopersici is environmentally safe, cost effective and does not disturb ecological balance. Investigations are in progress to test the efficacy of O. basilicum extract under in vivo conditions. PMID:24561899

  20. Plant poisoning in domestic animals: epidemiological data from an Italian survey (2000-2011).

    PubMed

    Caloni, F; Cortinovis, C; Rivolta, M; Alonge, S; Davanzo, F

    2013-06-01

    An Italian epidemiological study based on the human Poison Control Centre of Milan (Centro Antiveleni di Milano (CAV)) data related to domestic animal poisoning by exposure to plants, was carried out in collaboration with the Veterinary Toxicology Section of the University of Milan. It encompasses a 12-year period, from the beginning of 2000 to the end of 2011. Calls related to toxic plants accounted for 5.7 per cent of total inquiries (2150) received by CAV. The dog was the most commonly poisoned species (61.8 per cent of calls) followed by the cat (26 per cent). Little information was recorded for other species. Most exposures (73.8 per cent) resulted in mild to moderate clinical signs. The outcome was reported in only 53.7 per cent of cases, and fatal poisoning accounted for 10.6 per cent of these cases. Glycoside, alkaloid, oxalate, toxalbumin, saponin, terpene and terpenoid-containing plants were recorded and found to be responsible for intoxication. Cycas revoluta, Euphorbia pulcherrima, Hydrangea macrophylla, Nerium oleander, Rhododendron species and Prunus species were the plants most frequently involved. Epidemiological data from this Italian survey provide useful information on animal exposure to plants and confirm the importance of plants as causative agents of animal poisoning. PMID:23716536

  1. Caffeoylquinic acids in leaves of selected Apocynaceae species: Their isolation and content

    PubMed Central

    Wong, Siu Kuin; Lim, Yau Yan; Ling, Sui Kiong; Chan, Eric Wei Chiang

    2014-01-01

    Background: Three compounds isolated from the methanol (MeOH) leaf extract of Vallaris glabra (Apocynaceae) were those of caffeoylquinic acids (CQAs). This prompted a quantitative analysis of their contents in leaves of V. glabra in comparison with those of five other Apocynaceae species (Alstonia angustiloba, Dyera costulata, Kopsia fruticosa, Nerium oleander, and Plumeria obtusa), including flowers of Lonicera japonica (Japanese honeysuckle), the commercial source of chlorogenic acid (CGA). Materials and Methods: Compound were isolated by column chromatography, and identified by NMR and MS analyses. CQA content of leaf extracts was determined using reversed-phase HPLC. Results: From the MeOH leaf extract of V. glabra, 3-CQA, 4-CQA, and 5-CQA or CGA were isolated. Content of 5-CQA of V. glabra was two times higher than flowers of L. japonica, while 3-CQA and 4-CQA content was 16 times higher. Conclusion: With much higher CQA content than the commercial source, leaves of V. glabra can serve as a promising alternative source. PMID:24497746

  2. 33 CFR 80.1118 - Marina Del Rey, CA.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Marina Del Rey, CA. 80.1118... NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Coast 80.1118 Marina Del Rey, CA. (a) A line drawn from Marina Del Rey Breakwater South Light 1 to Marina Del Rey Light 4. (b) A line drawn from Marina Del...

  3. Applied clinical pharmacology and public health in rural Asia preventing deaths from organophosphorus pesticide and yellow oleander poisoning

    PubMed Central

    Eddleston, Michael

    2013-01-01

    Self-poisoning with pesticides or plants is a major clinical problem in rural Asia, killing several hundred thousand people every year. Over the last 17 years, our clinical toxicology and pharmacology group has carried out clinical studies in the North Central Province of Sri Lanka to improve treatment and reduce deaths. Studies have looked at the effectiveness of anti-digoxin Fab in cardiac glycoside plant poisoning, multiple dose activated charcoal in all poisoning, and pralidoxime in moderate toxicity organophosphorus insecticide poisoning. More recently, using a Haddon matrix as a guide, we have started conducting public health and animal studies to find strategies that may work outside of the hospital. Based on the 2009 GSK Research in Clinical Pharmacology prize lecture, this review shows the evolution of the group's research from a clinical pharmacology approach to one that studies possible interventions at multiple levels, including the patient, the community and government legislation. PMID:22943579

  4. Control del cáncer y salud mundial: noticia del Instituto Nacional del Cáncer (NCI)

    Cancer.gov

    En combinación con una reunión de alto nivel de las Naciones Unidas sobre enfermedades no transmisibles en países en vías de desarrollo, el doctor Harold Varmus, director del NCI, y el doctor Ted L. Trimble, del NCI, han publicado un comentario en Science

  5. Control del dolor: Apoyo para las personas con cncer

    Cancer.gov

    Libro sobre el control del dolor en pacientes con cncer. Cubre los mtodos y medicinas, as como los efectos fsicos y emocionales del dolor. Es una adaptacin cultural para el pblico de habla hispana del libro en ingls Pain Control.

  6. Líneas Vitales: Programas y servicios del NCI

    Cancer.gov

    Artículos y videos sobre los programas y servicios del Instituto Nacional del Cáncer de la serie educativa Líneas Vitales del NCI, la cual está dirigida especialmente a poblaciones multiculturales.

  7. Estudio del sistema simbitico AR Pavonis

    NASA Astrophysics Data System (ADS)

    Quiroga, C.; Brandi, E.; Ferrer, O.; Garca, L.; Barb, R.

    Se presenta un estudio espectroscpico y polarimtrico de la binaria eclipsante AR Pavonis a partir de observaciones obtenidas con el telescopio de 2.15 m. del CASLEO (San Juan). El estudio de la naturaleza del sistema se realiz a travs del anlisis de algunos espectros reunidos entre los aos 1990 y 1995 y de datos polarimtricos correspondientes al intervalo 1995 y 1997. El anlisis espectroscpico indica que AR Pav est compuesta por una gigante roja M3.7 y una componente caliente compacta, con una temperatura mayor a 90000 K y una luminosidad del orden de 500Lsolar. A lo largo del perodo orbital, las variaciones observadas en flujo y en velocidades radiales, reflejan que las emisiones permitidas se forman en una regin que rodea la componente caliente. El estudio de la polarizacin lineal de AR Pav indica que adems de una componente interestelar, existe una componente intrnseca del sistema que vara temporalmente y con la longitud de onda de la luz polarizada. El distinto comportamiento del grado de polarizacin y del ngulo de posicin observado en fases diferentes, sugiere que distintos mecanismos pueden estar actuando, dependiendo de la regin de scattering observado a lo largo de la lnea de la visual.

  8. Instantánea del linfoma

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el linfoma; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  9. Instantánea del sarcoma

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el sarcoma; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  10. Instantánea del melanoma

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el melanoma; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  11. Produccion Gaseosa del Cometa Halley: Erupciones Y Fotodisociacion del Radical OH

    NASA Astrophysics Data System (ADS)

    Silva, A. M.; Mirabel, I. F.

    1990-11-01

    RESUMEN:En este trabajo informamos la detecci6n de 20 erupciones en la li'nea de =18cm (1667MHz) del radical OH en el Cometa Halley.Las observaciones incluyen todos los monitoreos existentes y se extienden desde 120 dias antes del perihelio hasta 90 dias despues.Se detectan bruscos crecimientos en el flujo medido,hasta un factor 1O,seguidos por decaimientos lentos asociados con la fotodisociaci6n del OH. Se obtuvieron valores para el tiempo de vida fotoquimico del OH y del H2O basandose en el modelo desarrollado previamente por Silva(1988). Esos tiempos de vida estan de acuerdo con predicciones teoricas y con las observaciones en el Ultravioleta, y los resultados, los que son fuertemente dependientes de la velocidad heliocentrica del Coineta (variando hasta un factor 6), han sido calculados para varios rangos de velocidad entre +28 y -28 km/seg. Key wo'L :

  12. Gonadal dysgenesis in del (18p) syndrome

    SciTech Connect

    Telvi, L.; Ion, A.; Fouquet, F.

    1995-07-17

    We report on a girl with syndromal gonadal dysgenesis and a de novo del (18p). Genetic factors controlling gonadal development are located not only on the X chromosome, but also on autosomes. The present case suggests that one of these genes is situated on 18p. We conclude that patients with del (18p) syndrome should be evaluated for gonadal dysgenesis. 16 refs., 3 figs.

  13. Calidad de Imagen del Telescopio UNAM212

    NASA Astrophysics Data System (ADS)

    Cobos, F. J.; Teiada de Vargas, C.

    1987-05-01

    El telescopio UNAM2l2, del Observatorio Astronmico Nacional, situado en la Sierra de San Pedro Mrtir (Baja California, Mxico), cumplira en un futuro muy cercano siete aos de uso para fines de investigacin astronmica. Aunque en este tiempo no se ha efectuado un estudio sistemtico acerca de su comportamiento ptico y de los factores que influyen en la calidad de las imgenes, se han realizado pruebas diversas, estudios parciales y reuniones especificas, cuyos resultados no siempre se han difundido ampliamente y generalmente no se han presentado por escrito. Es por ello que hemos creido necesario intentar una recopilacin de la informacin existente para poder con ella establecer un diagnstjco que, aunque no sea definitivo, sirva de base para futuros trabajos tendientes a optimizar el comportamiento ptico del telescopio. Es evidente que un buen nmero de las conclusiones que se presentan son resultado del trabajo de muchas personas de esfuerzos colectivos. Asimismo, hemos tratado de localizar informacin bibliogrfica que pueda ser de utilidad. Nuestro objetivo primordial ha consistido en centrarnos en la ptica del telescopio y su calidad, pero tambin se han considerado otros aspectos que puedan afectar las imgenes obtenidas tales como: celda del primario, `seeing' local y externo, flexiones posibles en la estructura mecnica del telescopio, etc.

  14. Intertextual Sexual Politics: Illness and Desire in Enrique Gomez Carrillo's "Del amor", "del dolor y del vicio" and Aurora Caceres's "La rosa muerta"

    ERIC Educational Resources Information Center

    LaGreca, Nancy

    2012-01-01

    This study explores the intertextuality between Aurora Caceres's "La rosa muerta" (1914) and the novel "Del amor, del dolor y del vicio" (1898) by her ex-husband, Enrique Gomez Carrillo. Caceres strategically mentions Gomez Carrillo's novel in "La rosa muerta" to invite a reading of her work in dialogue with his. Both narratives follow the sexual…

  15. Intertextual Sexual Politics: Illness and Desire in Enrique Gomez Carrillo's "Del amor", "del dolor y del vicio" and Aurora Caceres's "La rosa muerta"

    ERIC Educational Resources Information Center

    LaGreca, Nancy

    2012-01-01

    This study explores the intertextuality between Aurora Caceres's "La rosa muerta" (1914) and the novel "Del amor, del dolor y del vicio" (1898) by her ex-husband, Enrique Gomez Carrillo. Caceres strategically mentions Gomez Carrillo's novel in "La rosa muerta" to invite a reading of her work in dialogue with his. Both narratives follow the sexual

  16. Digitalizacin de diapositivas del Sol en H ?

    NASA Astrophysics Data System (ADS)

    Missio, H.; Montenegro, C.; Montenegro, R.

    El objetivo de este trabajo ha sido el de obtener imgenes digitalizadas de las diapositivas tomadas del Sol en luz de hidrgeno de la lnea correspondiente a H?, y de esta manera llegar a convertir las mismas a un archivo digital para poder ser tratadas luego por computadora y poder contabilizar con exactitud, mediante un programa adecuado para tal fin, las zonas activas del Sol en la imagen digitalizada. En principio, para llegar a esto se pens en la utilizacin de medios accesibles, y como detector se utiliz un fototransistor ubicado dentro de un soporte rectangular sobre dos ejes de desplazamiento X e Y. Se han obtenido con este procedimiento imgenes de buena calidad, construdas a partir de tres datos digitalizados en cada barrido que aportan la posicin X e Y y la intensidad del pixel en ese punto indicada en 255 tonos de grises.

  17. Visiting the Gödel universe.

    PubMed

    Grave, Frank; Buser, Michael

    2008-01-01

    Visualization of general relativity illustrates aspects of Einstein's insights into the curved nature of space and time to the expert as well as the layperson. One of the most interesting models which came up with Einstein's theory was developed by Kurt Gödel in 1949. The Gödel universe is a valid solution of Einstein's field equations, making it a possible physical description of our universe. It offers remarkable features like the existence of an optical horizon beyond which time travel is possible. Although we know that our universe is not a Gödel universe, it is interesting to visualize physical aspects of a world model resulting from a theory which is highly confirmed in scientific history. Standard techniques to adopt an egocentric point of view in a relativistic world model have shortcomings with respect to the time needed to render an image as well as difficulties in applying a direct illumination model. In this paper we want to face both issues to reduce the gap between common visualization standards and relativistic visualization. We will introduce two techniques to speed up recalculation of images by means of preprocessing and lookup tables and to increase image quality through a special optimization applicable to the Gödel universe. The first technique allows the physicist to understand the different effects of general relativity faster and better by generating images from existing datasets interactively. By using the intrinsic symmetries of Gödel's spacetime which are expressed by the Killing vector field, we are able to reduce the necessary calculations to simple cases using the second technique. This even makes it feasible to account for a direct illumination model during the rendering process. Although the presented methods are applied to Gödel's universe, they can also be extended to other manifolds, for example light propagation in moving dielectric media. Therefore, other areas of research can benefit from these generic improvements. PMID:18989011

  18. Radio-Observaciones del OH EN la Coma del Cometa Halley Desde EL Hemisferio Sur

    NASA Astrophysics Data System (ADS)

    Silva, A. M.; Bajaja, E.; Morras, R.; Cersosimo, J. C.; Martin, M. C.; Arnal, E. M.; Poppel, W. G. L.; Colomb, F. R.; Mazzaro, J.; Olalde, J. C.; Boriakoff, V.; Mirabel, I. F.

    1987-05-01

    Se utilizó una antena de 30 metros del Instituto Argentino de Radioastronomía para observaciones diarias Cf ebrero a abril de 1986) de la transición en 1667 MHz ( λ = 18 cm) del OH en la coma del cometa Halley. De las observaciones realizadas se concluye: 1) El número promedio de moléculas de OH en la coma durante 37 días de observación fue de (8.9±3.5)x1034 moléculas, lo que implica una tasa de producción promedio de OH de 1.8x1029 moléculas seg-1 y consecuentemente una pérdida de masa promedio de 17±6 toneladas seg-1 . Este valor está de acuerdo con las mediciones realizadas por las sondas Vega y Giotto. 2) El monitoreo desde el lAR revela la existencia de variaciones bruscas en los flujos de absorción del OH. Estas variaciones son consistentes con los modelos que representan la producción gaseosa a partir de ejecciones y/o desprendimientos discretos de materia congelada del núcleo. 3) Las variaciones en la densidad de flujo son consistentes con las estimaciones de los tiem- pos de vida medios del H2O y del OH en presencia del campo de radiación solar. 4) Se encuentra una correlación entre la intensidad del flujo absorbido y anisotropías en Ia dinamica de la coma.

  19. PASO DEL NORTE SUMMER OZONE STUDY

    EPA Science Inventory

    The Paso del Norte Summer Ozone Study was designed to evaluate potential control strategies for their efficacy in reducing ozone and carbon monoxide concentrations in the El Paso-Ciudad Juarez-Sunland Park international airshed of Texas, New Mexico, and Chihuahua. A field study ...

  20. Andrea del Sarto rehabilitated: a psychoanalytic emendation.

    PubMed

    Trosman, Harry

    2002-01-01

    Ernest Jones's "The Influence of Andrea del Sarto's Wife on His Art" (1913) is an early example of psychoanalysis applied to the study of a prominent painter. Greatly influenced by Freud's Leonardo da Vinci and a Memory of His Childhood, Jones gave excessive credence to Vasari's highly prejudicial account of the life of del Sarto, on which the study relied heavily. Jones attempted to account psychologically for the circumstance that del Sarto, though highly skilled and "faultless," was not the equal of the three preeminent masters of the Italian High Renaissance: Leonardo, Michelangelo, and Raphael. Jones's uncritical acceptance of the Vasari biography encouraged him to view Sarto's assumed deficiency as the result of excessive attachment to his wife, a pathological uxoriousness. A contemporary psychoanalytic perspective, with its emphasis on the emotive response of the analyst, requires us to pay attention to the evocative nature of the work of the artist, an approach Jones neglected. In an examination of several paintings, the artist's sensitivity to the position of the spectator is explored, as is the interest in involving the viewer spatially and emotionally. An appreciation for the viewer's position is consistent with a capacity for using projected internal objects for creative purposes. The presence of this capacity suggests a revised view of del Sarto's contribution to art and of his relationship with his wife. PMID:12580329

  1. Procesamiento Digital de Imagenes del Cometa Halley

    NASA Astrophysics Data System (ADS)

    Ferrin, L.; Fuenmayor, F.; Naranjo, O.; Bulka, P.; Mendoza, C.

    1987-05-01

    Se reportan observaciones fotogrficas del cometa Halley, obtenidas con los telescopios Schmidt de 1-m del CIDA, y de 35 cms de la ULA. Se hicieron exposiciones desde 2 segundos a 30 minutos y se utilizaron emulsiones IIa-O, 103a-F, y 103a-D, guladas manualmente 0 automaticmente. Las imgenes fueron digitalizadas con el microdensitmetro PDS, y procesadas con el sistema HACIENDA del CCIBM. Se experiment con la Transformada de Fourier en dos dimensiones, y con la aplicacin de filtros de paso alto y bajo. Se encontr que el metodo de "autocorrelacin" es el mejor para separar "la vegetacin" de "la montaa". Se aplicaron diversas tcnicas a fin de cubrir ambos extremos: a) enfatizar detalles dbiles en la cola, y b) penetrar en las regiones ms intensas de la coma. Se lograron ambos objetivos. Detalles en la cola permitieron determinar velocidades de propagacin de unos 50 a 90 kms/ seg. Se pudieron detectar no menos de tres perturbaciones en "Y", y una en 5? Co de Cisne). Se cree que las primeras estn asociadas a eventos de desconexin. Se puede separar la cola de gas de la de polvo. Las fotos de color permiten enfatizar diferentes regiones espectrales con mayor claridad an. El "balance" del color puede ser hecho con la computadora.

  2. Near Infrared Photometry of Nova Del 2013

    NASA Astrophysics Data System (ADS)

    Cass, A. C.; Carlon, R. L.; Corgan, D. T.; Dykhoff, D. A.; Gehrz, R. D.; Shenoy, D. P.

    2013-09-01

    We continued to follow Nova Del 2013 during the period from 31.15 August 2013 UT through 25.18 September 2013 UT using an As:Si bolometer mounted on the 0.76-m infrared telescope of the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA). Vega (alpha Lyrae) is used as the standard star.

  3. Comportamiento del Helio en estrellas qumicamente peculiares

    NASA Astrophysics Data System (ADS)

    Malaroda, S. M.; Lpez Garca, Z.; Leone, F.; Catalano, F.

    Las estrellas qumicamente peculiares (CP) se caracterizan por tener deficiencias y sobreabundancias de algunos elementos qumicos de hasta 106 veces la abundancia solar. Adems presentan variaciones en las lneas espectrales. Se piensa que ello se debe a que los campos magnticos presentes en este tipo de estrellas son principalmente dipolares, con un eje de simetra diferente del eje de rotacin. La distribucin de los elementos sobreabundantes y deficientes no es homognea sobre la superficie estelar y las variaciones observadas seran una consecuencia directa de la rotacin estelar. Entre los elementos con abundancia anmala se encuentra el Helio, cuyas lneas tienen intensidades que no son consistentes con una abundancia normal, que no puede ser determinada del modo usual, o sea, considerando una atmsfera con composicin solar. Con el fin de determinar la abundancia de este elemento, se inici un estudio de estrellas anmalas de Helio, Hew y He strong. Adems se determinarn las abundancias de otros elementos anmalos como ser el Si, Cr, Mg, Mn y Fe. Las mismas se determinan del modo tradicional, o sea: a) medida de los anchos equivalentes de las lneas de los distintos elementos analizados; b) adopcin de la temperatura efectiva, gravedad y abundancia del Helio; c) clculo del modelo de atmsfera d) comparacin con las observaciones y reinicio de un proceso iterativo hasta lograr un acuerdo entre todos los parmetros analizados. Las observaciones se llevaron a cabo en el Complejo Astronmico El Leoncito. Se observaron setenta y ocho estrellas anmalas de Helio. En este momento se est procediendo a calcular las abundancias correspondientes a los distintos elementos qumicos. Para ello se hace uso de los modelos de Kurucz, ATLAS9. Los clculos NLTE de las lneas de Helio se llevan a cabo con el programa MULTI y se compararn con los realizados con el programa WIDTH9 de Kurucz (LTE), con el objeto de resaltar la importancia de los efectos NLTE.

  4. Vigilando la Calidad del Agua de los Grandes Rios de la Nacion: El Programa NASQAN del Rio Grande (Rio Bravo del Norte)

    USGS Publications Warehouse

    Lurry, Dee L.; Reutter, David C.; Wells, Frank C.; Rivera, M.C., (translator); Munoz, A.

    1998-01-01

    La Oficina del Estudio Geologico de los Estados Unidos (U.S. Geological Survey, 0 USGS) ha monitoreado la calidad del agua de la cuenca del Rio Grande (Rio Bravo del Norte) desde 1995 como parte de la rediseiiada Red Nacional para Contabilizar la Calidad del Agua de los Rios (National Stream Quality Accounting Network, o NASOAN) (Hooper and others, 1997). EI programa NASOAN fue diseiiado para caracterizar las concentraciones y el transporte de sedimento y constituyentes quimicos seleccionados, encontrados en los grandes rios de los Estados Unidos - incluyendo el Misisipi, el Colorado y el Columbia, ademas del Rio Grande. En estas cuatro cuencas, el USGS opera actualmente (1998) una red de 40 puntos de muestreo pertenecientes a NASOAN, con un enfasis en cuantificar el flujo en masa (la cantidad de material que pasa por la estacion, expresado en toneladas por dial para cada constituyente. Aplicacando un enfoque consistente, basado en la cuantificacion de flujos en la cuenca del Rio Grande, el programa NASOAN esta generando la informacion necesaria para identificar fuentes regionales de diversos contaminantes, incluyendo sustancias qui micas agricolas y trazas elementos en la cuenca. EI efecto de las grandes reservas en el Rio Grande se puede observar segun los flujos de constituyentes discurren a 10 largo del rio. EI analisis de los flujos de constituyentes a escala de la cuenca proveera los medios para evaluar la influencia de la actividad humana sobre las condiciones de calidad del agua del Rio Grande.

  5. Clinical and laboratory update on the DEL variant.

    PubMed

    Nuchnoi, Pornlada; Thongbus, Jairak; Srisarin, Apapan; Kerdpin, Usanee; Prachayasittikul, Virapong

    2014-01-01

    Serological assays for the RhD blood group are based on detection of the RhD antigen on human red blood cells using a specific anti-D antibody. The weak expression of the RhD antigen in the DEL variant hinders the sensitivity of conventional serological assays. Evidence of anti-D immunization in patients with D-negativity who have received DEL-variant blood units has been reported in various populations. This observation has prompted the need for genetic epidemiological and clinical data on the DEL variant in the development of DEL molecular diagnostic testing. This review highlights the molecular features of the DEL variant, the clinical consequences of DEL-blood transfusion, and current approaches for detection of the DEL-variant for donor screening and transfusion. PMID:25316658

  6. Del Norte means north to recycling

    SciTech Connect

    Aquino, J.T.

    1998-06-01

    Del Norte Regional Recycling and Transfer Station is owned by the city of Oxnard, California and operated by BLT Enterprises, Inc. The Del Norte facility--located in southwestern Ventura County about an hour northwest of Los Angeles--processes polyethylene terephthalate (PET) and high-density polyethylene (HDPE) plastic containers, aluminum, steel, glass, old corrugated containers (OCC), newspapers, computer printout paper, white and colored ledger paper, coated book, supermix paper, telephone books, and old magazines. According to the company, there has been virtually no community opposition to the site. The facility has few neighbors, and those are agricultural. To keep the community relationship strong, the facility`s design and location all but eliminated odor and noise complaints. The building was designed against the prevailing wind pattern, and BLT processes odorous material fast. A misting system installed for dust suppression also can be used with a solution for odor control should the need arise.

  7. Morphology, geology and geochemistry of the "Salar del Gran Bajo del Gualicho" (Rio Negro, Argentina)

    USGS Publications Warehouse

    Angelucci, A.; Barbieri, M.; Brodtkorb, A.; Ciccacci, S.; Civitelli, G.; De Barrio, R.; Di, Filippo M.; Fredi, P.; Friedman, I.; Lombardi, S.; Schalamuk, A.I.; Toro, B.

    1996-01-01

    A multidisciplinary study of the Gran Bajo del Gualicho area (Rio Negro - Argentina) was carried out; the aim was to delineate its geological and geomorphological evolution and to estabilish the genesis of salts filling the depression. Climatic conditions were analized first to individuate their role in the present morphogenetic processes; moreover the main morphological features of present landscape were examined as well as the stratigraphy of the outcropping formations, and of the Gran Bajo del Gualicho Formation in particular. Finally, a possible geomorphological evolution of the studied area was traced. Geophysical analyses allowed to estabilish that the paleosurface shaped on the crystalline basement is strongly uneven and shows evidence of the strong tectonic phases it underwent. The result of isotope analyses confirmed that the salt deposits on the Gran Bajo del Gualicho bottom were produced by fresh water evaporation, while strontium isotope ratio suggested that such waters were responsible for solubilization of more ancient evaporitic deposits.

  8. Ethnopharmacological survey of wild medicinal plants in Showbak, Jordan.

    PubMed

    Al-Qura'n, S

    2009-05-01

    Two main research questions are framing this investigation: (1) the main taxa of the medicinal importance value altered the Showbak forest stand and species composition? (2) The most safe species and what are the toxic ones (unsafe). These two research questions are the vital ones to draw a clear image about the wild medicinal plants of this investigated area of Showbak region in Jordan. 79 wild medicinal plant species were investigated in this study which are used in traditional medication for the treatment of various diseases. Most of the locals interviewed dealt with well-known safe medicinal plants such as Aaronsohnia factorovskyi Warb. et Eig., Achillea santolina L., Adiantum capillus-veneris L., Artemisia herba-alba L., Ceratonia siliqua L., Clematis recta L., Herniaria hirsuta L., Malva neglecta Wallr., Rosmarinus officinalis L., Ruta chalepensis L., Salvia triloba L., Sarcopoterium spinosa (L.) Spach., Thymbra capitata (L.) Hof, and Urginea maritima Barker. Many of the wild medicinal plants investigated were toxic and needed to be practiced by practitioners and herbalists rather than the local healers. These plants include Calotropis procera Willd R.Br., Citrullus colocynthis (L.) Sch., Datura stramonium L., Digitalis purpurea L., Ecballium elaterium (L.) A.Rich., Euphorbia helioscopia L., Euphorbia tinctoria Boiss., Glaucium corniculatum (L.) Curt., Hyoscyamus aureus L., Mandragora officinarum L., Nerium oleander L., Ricinus communis L., Solanum nigrum L., Withania somnifera (L.) Dunel. The conservation of medicinal plants and natural resources is becoming increasingly important, so this research is trying to collect information from local population concerning the use of medicinal plants in Showbak; identify the most important specie; determine the relative importance value of the species and calculate the informant consensus factor (ICF) for the medicinal plants. Obtaining results is relied on the interviewee's personal information and the medicinal use of specific plants. PMID:19429338

  9. Application of optical spectroscopic techniques for disease diagnosis

    NASA Astrophysics Data System (ADS)

    Saha, Anushree

    Optical spectroscopy, a truly non-invasive tool for remote diagnostics, is capable of providing valuable information on the structure and function of molecules. However, most spectroscopic techniques suffer from drawbacks, which limit their application. As a part of my dissertation work, I have developed theoretical and experimental methods to address the above mentioned issues. I have successfully applied these methods for monitoring the physical, chemical and biochemical parameters of biomolecules involved in some specific life threatening diseases like lead poisoning and age-related macular degeneration (AMD). I presented optical studies of melanosomes, which are one of the vital organelles in the human eye, also known to be responsible for a disease called age-related macular degeneration (AMD), a condition of advanced degeneration which causes progressive blindness. I used Raman spectroscopy, to first chemically identify the composition of melanosome, and then monitor the changes in its functional and chemical behavior due to long term exposure to visible light. The above study, apart from explaining the role of melanosomes in AMD, also sets the threshold power for lasers used in surgeries and other clinical applications. In the second part of my dissertation, a battery of spectroscopic techniques was successfully applied to explore the different binding sites of lead ions with the most abundant carrier protein molecule in our circulatory system, human serum albumin. I applied optical spectroscopic tools for ultrasensitive detection of heavy metal ions in solution which can also be used for lead detection at a very early stage of lead poisoning. Apart from this, I used Raman microspectroscopy to study the chemical alteration occurring inside a prostate cancer cell as a result of a treatment with a low concentrated aqueous extract of a prospective drug, Nerium Oleander. The experimental methods used in this study has tremendous potential for clinical application and will gain widespread acceptance within next few years from bench to bedside as an inexpensive and non-invasive tool compared to the other technologies.

  10. Copper, zinc and lead biogeochemistry in aquatic and land plants from the Iberian Pyrite Belt (Portugal) and north of Morocco mining areas.

    PubMed

    Durães, Nuno; Bobos, Iuliu; Ferreira da Silva, Eduardo; Dekayir, Abdelilah

    2015-02-01

    The ability of aquatic (Juncus effusus L., Scirpus holoschoenus L., Thypha latifolia L. and Juncus sp.) and land (Cistus ladanifer L., Erica andevalensis C.-R., Nerium oleander L., Isatis tinctoria L., Rosmarinus officinalis L., Cynodon dactylon L. and Hordeum murinum L.) plants from Portugal (Aljustrel, Lousal and São Domingos) and Morocco (Tighza and Zeida) mining areas to uptake, translocate and tolerate heavy metals (Cu, Zn and Pb) was evaluated. The soils (rhizosphere) of the first mining area are characterized by high acidity conditions (pH 2-5), whereas from the second area, by alkaline conditions (pH 7.0-8.5). Physicochemical parameters and mineralogy of the rhizosphere were determined from both areas. Chemical analysis of plants and the rhizosphere was carried out by inductively coupled plasma emission spectrometry. The sequential chemical extraction procedure was applied for rhizosphere samples collected from both mining areas. In the acid conditions, the aquatic plants show a high capacity for Zn bioaccumulation and translocation and less for Pb, reflecting the following metal mobility sequence: Zn > Cu > Pb. Kaolinite detected in the roots by infrared spectroscopy (IR) contributed to metal fixation (i.e. Cu), reducing its translocation to the aerial parts. Lead identified in the roots of land plants (e.g. E. andevalensis) was probably adsorbed by C-H functional groups identified by IR, being easily translocated to the aerial parts. It was found that aquatic plants are more efficient for phytostabilization than bioaccumulation. Lead is more bioavailable in the rhizosphere from Morocco mining areas due to scarcity of minerals with high adsorption ability, being absorbed and translocated by both aquatic and land plants. PMID:25167810

  11. Mistletoes and mutant albino shoots on woody plants as mineral nutrient traps

    PubMed Central

    Lo Gullo, M. A.; Glatzel, G.; Devkota, M.; Raimondo, F.; Trifilò, P.; Richter, H.

    2012-01-01

    Background and Aims Potassium, sulphur and zinc contents of mistletoe leaves are generally higher than in their hosts. This is attributed to the fact that chemical elements which are cycled between xylem and phloem in the process of phloem loading of sugars are trapped in the mistletoe, because these parasites do not feed their hosts. Here it is hypothesized that mutant albino shoots on otherwise green plants should behave similarly, because they lack photosynthesis and thus cannot recycle elements involved in sugar loading. Methods The mineral nutrition of the mistletoe Scurrula elata was compared with that of albino shoots on Citrus sinensis and Nerium oleander. The potential for selective nutrient uptake by the mistletoe was studied by comparing element contents of host leaves on infected and uninfected branches and by manipulation of the haustorium–shoot ratio in mistletoes. Phloem anatomy of albino leaves was compared with that of green leaves. Key Results Both mistletoes and albino leaves had higher contents of potassium, sulphur and zinc than hosts or green leaves, respectively. Hypothetical discrimination of nutrient elements during the uptake by the haustorium is not supported by our data. Anatomical studies of albino leaves showed characteristics of release phloem. Conclusions Both albino shoots and mistletoes are traps for elements normally recycled between xylem and phloem, because retranslocation of phloem mobile elements into the mother plant or the host is low or absent. It can be assumed that the lack of photosynthetic activity in albino shoots and thus of sugars needed in phloem loading is responsible for the accumulation of elements. The absence of phloem loading is reflected in phloem anatomy of these abnormal shoots. In mistletoes the evolution of a parasitic lifestyle has obviously eliminated substantial feeding of the host with photosynthates produced by the mistletoe. PMID:22442343

  12. Contribution to the knowledge of the veterinary science and of the ethnobotany in Calabria region (Southern Italy)

    PubMed Central

    Passalacqua, Nicodemo G; De Fine, Giuseppe; Guarrera, Paolo Maria

    2006-01-01

    Background A series of preliminary research projects on plants used in Calabria (Southern Italy) in veterinary science and in other ethno-botanical fields (minor nourishment, domestic and handicraft sector) was carried out in the last twenty years. From the ethno-botanical point of view, Calabria is one of the most interesting region, since in the ancient times it was subject to the dominant cultures of several people (Greeks, Romans, Byzantines, Arabs, Normans etc.). Until some decades ago the road network was poorly developed and villages were isolated, so that the culture of the "subsistence" and some archaic customs were kept. Methods Data were collected by means of "open" interviews to farmers, shepherds and housewives in the last twenty years. More than 100 informants were interviewed, mostly over 50 years old. Plants were identified by local informants through gathering in the area or through examination of the fresh plants collected by the researchers. The collected data were compared with pharmacobotanical papers mainly of southern Italy and with other studies, in order to highlight novelties or concordances of uses. Results The use of 62 taxa distributed into 34 families are described. Among these, 8 are or were employed in veterinary science, 8 as anti-parasitic agents, 19 in minor nourishment, 5 as seasoning, 38 for other uses. Some toxic species for cattle are also mentioned. Conclusion Among the major findings: the use of Helleborus bocconei for bronchitis of bovines and of Scrophularia canina for lameness in veterinary science; Nerium oleander and Urginea maritima as anti-parasitic agents; Epilobium angustifolium, Centaurea napifolia L. and C. sphaerocephala L. in minor nourishment. PMID:17156472

  13. Del-1 overexpression potentiates lung cancer cell proliferation and invasion.

    PubMed

    Lee, Seung-Hwan; Kim, Dong-Young; Jing, Feifeng; Kim, Hyesoon; Yun, Chae-Ok; Han, Deok-Jong; Choi, Eun Young

    Developmental endothelial locus-1 (Del-1) is an endogenous anti-inflammatory molecule that is highly expressed in the lung and the brain and limits leukocyte migration to these tissues. We previously reported that the expression of Del-1 is positively regulated by p53 in lung endothelial cells. Although several reports have implicated the altered expression of Del-1 gene in cancer patients, little is known about its role in tumor cells. We here investigated the effect of Del-1 on the features of human lung carcinoma cells. Del-1 mRNA was found to be significantly decreased in the human lung adenocarcinoma cell lines A549 (containing wild type of p53), H1299 (null for p53) and EKVX (mutant p53), compared to in human normal lung epithelial BEAS-2B cells and MRC-5 fibroblasts. The decrease of Del-1 expression was dependent on the p53 activity in the cell lines, but not on the expression of p53. Neither treatment with recombinant human Del-1 protein nor the introduction of adenovirus expressing Del-1 altered the expression of the apoptosis regulators BAX, PUMA and Bcl-2. Unexpectedly, the adenovirus-mediated overexpression of Del-1 gene into the lung carcinoma cell lines promoted proliferation and invasion of the lung carcinoma cells, as revealed by BrdU incorporation and transwell invasion assays, respectively. In addition, overexpression of the Del-1 gene enhanced features of epithelial-mesenchymal transition (EMT), such as increasing vimentin while decreasing E-cadherin in A549 cells, and increases in the level of Slug, an EMT-associated transcription regulator. Our findings demonstrated for the first time that there are deleterious effects of high levels of Del-1 in lung carcinoma cells, and suggest that Del-1 may be used as a diagnostic or prognostic marker for cancer progression, and as a novel therapeutic target for lung carcinoma. PMID:26545781

  14. Manual del espectrgrafo multifuncin de Bosque Alegre

    NASA Astrophysics Data System (ADS)

    Daz, R.; Paolantonio, S.; Carranza, G.; Goldes, G.

    Este manual consta de 120 pginas y resume las caractersticas del Espectrgrafo Multifuncin instalado en el telescopio de 1.54 m de la Estacin Astrofsica de Bosque Alegre. En este trabajo se describen, adems, las tcnicas a emplear para su puesta a punto y la secuencia de operaciones para la obtencin de datos en cada uno de los modos que ya se encuentran en funcionamiento. Parte de este trabajo se realiz con el apoyo de la Asociacin Argentina de Astronoma, a travs de becas otorgadas a R.D. y S.P..

  15. Near Infrared Photometry of Nova Del 2013

    NASA Astrophysics Data System (ADS)

    Cass, C. A.; Carlon, L. R.; Corgan, T. D.; Dykhoff, A. D.; Gehrz, D. R.; Shenoy, P. D.

    2013-08-01

    Subjects: Infra-Red, Nova We report RIJHKLM photometry of Nova Del 2013 taken on August 28.13 UT using an As:Si bolometer mounted on the 0.76-m infrared telescope of the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA). Vega (alpha Lyrae) was used as the standard star. On this date we find: R = 5.6 +/- 0.1, I = 5.2 +/- 0.1, J = 4.5 +/- 0.1, H = 4.7 +/- 0.1, K = 4.7 +/- 0.1, L = 3.3 +/- 0.2, M = +1.8 +/- 0.3.

  16. Huave de San Mateo del Mar, Oaxaca (Huave of San Mateo del Mar, Oaxaca).

    ERIC Educational Resources Information Center

    Mexico Coll. (Mexico City)

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Huave, an indigenous language of Mexico spoken in San Mateo del Mar, in the state of Oaxaca. The objective of collecting such a representative sampling of

  17. 77 FR 50080 - Del Norte County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-20

    ... Forest Service Del Norte County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice... recommendations to the Forest Service concerning projects and funding consistent with the title II of the Act. The... at the Del Norte County Unified School District, Redwood Room, 301 West Washington...

  18. 75 FR 63437 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... Forest Service Del Norte Resource Advisory Committee (RAC) AGENCY: Forest Service, USDA. ACTION: Notice... Del Norte County Unified School District, Redwood Room, 301 West Washington Boulevard, Crescent City... Forest, at (707) 441-3673; e-mail: jranieri@fs.fed.us . SUPPLEMENTARY INFORMATION: The meeting is open...

  19. 76 FR 8333 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-14

    ... Forest Service Del Norte Resource Advisory Committee (RAC) AGENCY: Forest Service, USDA. ACTION: Notice... at the Del Norte County Unified School District, Redwood Room, 301 West Washington Boulevard..., Six Rivers National Forest, at (707) 441-3569; e-mail adellinger@fs.fed.us . SUPPLEMENTARY...

  20. 75 FR 78966 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-17

    ... Forest Service Del Norte Resource Advisory Committee (RAC) AGENCY: Forest Service, USDA. ACTION: Notice... Del Norte County Unified School District, Redwood Room, 301 West Washington Boulevard, Crescent City... Forest, at (707) 441-3569; e-mail adellinger@fs.fed.us . SUPPLEMENTARY INFORMATION: The meeting is...

  1. 76 FR 13124 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ... Forest Service Del Norte Resource Advisory Committee (RAC) AGENCY: Forest Service, USDA. ACTION: Notice... Norte County Unified School District, Redwood Room 301, West Washington Boulevard, Crescent City... National Forest, at (707) 441-3569; e-mail adellinger@fs.fed.us . SUPPLEMENTARY INFORMATION: The Del...

  2. Instantánea del sarcoma de Kaposi

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el sarcoma de Kaposi; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  3. 33 CFR 110.65 - Indian River Bay, Del.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Indian River Bay, Del. 110.65 Section 110.65 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas 110.65 Indian River Bay, Del. Beginning at a point...

  4. Pastoral del Nino: Bringing the Abundant Life to Paraguayan Children

    ERIC Educational Resources Information Center

    Austin, Ann Berghout; Aquino, Cyle; Burro, Elizabeth

    2007-01-01

    Pastoral del Nino is transforming children's lives in rural Paraguay. Part of Pastoral Social (Catholic Social Services), Pastoral del Nino's primary focus is to bring "vida en abundancia" (the abundant life) to families by ensuring that mothers survive childbirth and children reach their first birthdays. In addition, the organization promotes…

  5. Formacin del contnuo de Lyman en atmsferas de estrellas B

    NASA Astrophysics Data System (ADS)

    Rohrmann, R. D.

    Se presentan resultados de modelos de atmsferas para estrellas tempranas que poseen una estructura cromosfrica y una atmsfera extendida en expansin. Se analiza con rigurosidad el acople del campo de radiacin en el continuo de Lyman y su relacin con las poblaciones en equilibrio estadstico del H y He.

  6. Instantánea del cáncer colorrectal

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer colorrectal; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  7. Pastoral del Nino: Bringing the Abundant Life to Paraguayan Children

    ERIC Educational Resources Information Center

    Austin, Ann Berghout; Aquino, Cyle; Burro, Elizabeth

    2007-01-01

    Pastoral del Nino is transforming children's lives in rural Paraguay. Part of Pastoral Social (Catholic Social Services), Pastoral del Nino's primary focus is to bring "vida en abundancia" (the abundant life) to families by ensuring that mothers survive childbirth and children reach their first birthdays. In addition, the organization promotes

  8. Se evitaron casi 800 000 muertes por descenso del tabaquismo

    Cancer.gov

    Programas y estrategias de control del tabaco del siglo XX fueron responsables de la prevencin de ms de 795 000 muertes por cncer de pulmn en Estados Unidos de 1975 al 2000. Si todo el tabaquismo en este pas hubiera cesado despus de la publicacin d

  9. [Strengths and future of the Revista Mdica del Instituto Mexicano del Seguro Social].

    PubMed

    Fajardo-Dolci, Germn

    2014-01-01

    The journals of medicine arose as a communication tool more than 200 years ago. At the beginning, their nature was local; later, their aim was to spread medical information along the nation; and, finally, they sought to reach the world distribution. The Revista Mdica del Instituto Mexicano del Seguro Social was published for the first time 52 years ago, and it has walked its way from local to international distribution. This journal has 23 000 subscribers, it is included in Medline and it reached a 0.112 SCImago Journal Rank in 2012. Its website receives around 200 000 visits monthly and 45 % are foreign visits. In the future, the peer review system is going to be strengthened, and the journal is going to offer audio, video, and applications to reinforce interactive participation between authors, readers in order to reach modernity and draw young new attention. PMID:24758842

  10. Illuminating water and life: Emilio Del Giudice.

    PubMed

    Ho, Mae-Wan

    2015-01-01

    The quantum electrodynamics theory of water put forward by Del Giudice and colleagues provides a useful foundation for a new science of water for life. The interaction of light with liquid water generates quantum coherent domains in which the water molecules oscillate between the ground state and an excited state close to the ionizing potential of water. This produces a plasma of almost free electrons favoring redox reactions, the basis of energy metabolism in living organisms. Coherent domains stabilized by surfaces, such as membranes and macromolecules, provide the excited interfacial water that enables photosynthesis to take place, on which most of life on Earth depends. Excited water is the source of superconducting protons for rapid intercommunication within the body. Coherent domains can also trap electromagnetic frequencies from the environment to orchestrate and activate specific biochemical reactions through resonance, a mechanism for the most precise regulation of gene function. PMID:26098522

  11. Estudio teórico del CO2. Orbitales de valencia y del ``core''

    NASA Astrophysics Data System (ADS)

    Olalla Gutiérrez, E.

    Hemos calculado las intensidades de las transiciones E1 a los miembros de las series de Rydberg con origen en los orbitales ``no enlazantes'' del dióxido de carbono, especie de conocida relevancia atmosférica. Se han computado, asimismo, los continuos de fotoionización correspondientes a los distintos canales de ionización, representándolos como densidad espectral de fuerza de oscilador frente a la energía del fotón incidente; mostramos los resultados df/dE para la fotoionización total de esta especie en el intervalo 15-60 eV. Todos los cálculos se han llevado a cabo mediante la formulación Molecular del Método de los Orbitales de Defecto Cuántico, MQDO [1,2]. La calidad de los resultados que presentamos se ha evaluado en base a la comparación con los datos, tanto experimentales como teóricos, disponibles en la bibliografía. El acuerdo encontrado es altamente satisfactorio

  12. Centro para la Salud Mundial (CGH) del NCI

    Cancer.gov

    El Centro para la Salud Mundial (CGH) del NCI coordina actividades de investigación y trabaja con socios nacionales e internacionales para comprender y enfrentar la carga que representa el cáncer a nivel mundial.

  13. PASO DEL NORTE HOTEL, 115117 S. EL PASO ST., SOUTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PASO DEL NORTE HOTEL, 115-117 S. EL PASO ST., SOUTH AND EAST FACADES, WEST SIDE OF STREET - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  14. 88. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    88. Paso Del Norte Hotel, 115-117 South El Paso St., south facade, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  15. 92. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    92. Paso Del Norte Hotel, 115-117 South El Paso St., interior, lobby rotunda - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  16. PASO DEL NORTE HOTEL, 115117 S. EL PASO ST., EAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PASO DEL NORTE HOTEL, 115-117 S. EL PASO ST., EAST FACADE, WEST SIDE OF STREET - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  17. 91. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    91. Paso Del Norte Hotel, 115-117 South El Paso St., east facade, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  18. 65. Photographer unknown March 1931 DEL NORTE COUNTY, SECTION B, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    65. Photographer unknown March 1931 DEL NORTE COUNTY, SECTION B, HIGHWAY 1, 1-DN-1-B #66A, STA. 166.0+00 WIDENED ROADBED BEFORE COVERING LOG CRIB, 3-31. BACK READS: WIDENING ROAD BED WITH LOGS, APPROX 30 SQUARE & 60 LONG ON DN-1 SEC B STA 156+. Stamped office copy, - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  19. Se evitaron casi 800 000 muertes por descenso del tabaquismo

    Cancer.gov

    Programas y estrategias de control del tabaco del siglo XX fueron responsables de la prevención de más de 795 000 muertes por cáncer de pulmón en Estados Unidos de 1975 al 2000. Si todo el tabaquismo en este país hubiera cesado después de la publicación d

  20. Archivo de placas astromtricas del Observatorio de La Plata

    NASA Astrophysics Data System (ADS)

    di Sisto, R.; Orellana, R. B.

    Se ha realizado una base de datos con las placas fotogrficas obtenidas con el Astrogrfico del Observatorio de La Plata. Se han clasificado un total de 3000 placas obtenidas para asteroides y cometas. El acceso a la base de datos se har por FTP y la misma contendr la siguiente informacin: fecha y tiempo de exposicin, coordenadas del centro de placa, tipo de emulsin fotogrfica, estado de la placa, objeto fotografiado.

  1. Mejoras en la exactitud del reloj de ngulo horario del telescopio de 2,15 mts de CASLEO

    NASA Astrophysics Data System (ADS)

    Aballay, J. L.; Pereyra, P. F.; Marn, A. H.

    Para aumentar la exactitud en el control del ngulo horario del telescopio, se est implementando el uso de un reloj con una precisin de 1/100 seg. En conjunto con el encoder que otorga la posicin con un acierto de 0,012 seg. de arco, se podr implementar otro dgito en el reloj de ngulo horario con la posibilidad de ver las dcimas. Esto, sumado a la precisin ya lograda en declinacin, permitir realizar offsets con mayor exactitud.

  2. Modelo semi-emprico de protuberancia solar a partir del diagnstico de densidades

    NASA Astrophysics Data System (ADS)

    Cirigliano, D.; Vial, J. C.; Rovira, M.

    A partir de la observacin del espectro del quintuplete de C III alrededor de 1175 , se ha realizado el diagnstico de la densidad y presin electrnica, basado en el clculo del cociente de las intensidades observadas. Una vez establecida la densidad electrnica, y con el clculo de las velocidades Doppler, hemos investigado el flujo de masa en la protuberancia en funcin de la temperatura. Estableciendo como hiptesis la conservacin del nmero de partculas que ingresan y salen del cuerpo de la protuberancia, se investiga la variacin del rea de un tubo de flujo semi-emprico en funcin de la temperatura. A partir de dicho diagnstico, se examina el comportamiento del radio del tubo magntico en funcin de la temperatura, los que dan cuenta de la abertura de las lneas de campo magntico que confinan el plasma y de la divergencia del campo magntico en diferentes alturas de la atmsfera solar.

  3. El diseo final del espectrgrafo de banco (EBASIM) para CASLEO

    NASA Astrophysics Data System (ADS)

    Simmons, J.; Levato, H.

    Utilizando el cdigo de ptica ACCOS V se ha finalizado el diseo del espectrgrafo de banco para CASLEO. En una comunicacin anterior habamos indicado que utilizaramos un colimador de 150 mm de dimetro con un radio de curvatura de 1540 mm. Para el espejo cmara, que tiene un dimetro de 200 mm, el radio de curvatura es de 1200 mm, ambos radios con una tolerancia no mayor a los 3 mm. En la presente, se informa sobre los detalles finales del clculo del espectrgrafo que incluye el cmputo para 5 longitudes de onda diferentes y alrededor de 100 rayos. En todos los casos el 75 % de energa est dentro de un dimetro de 13 micrones. El diseo ha sido probado entre 3500 hasta 9000 con resultados satisfactorios.

  4. Estudio multifrecuencia del medio interestelar cercano a HD 192281

    NASA Astrophysics Data System (ADS)

    Arnal, E. M.; Cappa, C. E.; Cichowolski, S.; Pineault, S.; St-Louis, N.

    Una de las causas que modifica la estructura y dinmica del medio interestelar es la accin que los vientos de las estrellas de gran masa ejercen sobre el mismo. En este trabajo, mediante el uso de datos interferomtricos obtenidos en la banda de radio en la transicin de ?21-cm del hidrgeno neutro y de imgenes de la emisin de continuo en las bandas de 408 y 1420 MHz, de imgenes HIRES del satlite IRAS en 60 y 100?m, y de observaciones de continuo obtenidas con radiotelescopios de disco simple en 2695, 4850 y 8350 MHz se ha realizado un estudio multifrecuencia de los efectos que los vientos estelares de HD 192281, una estrella de tipo espectral O5,Vn((f))p, han tenido sobre el medio interestelar que rodea a la misma.

  5. Estudio multifrecuencia del medio interestelar cercano a HD 192281

    NASA Astrophysics Data System (ADS)

    Arnal, E. M.; Cappa, C.; Cichowolski, S.; Pineault, S.; St-Louis, N.

    Una de las causas que modifica la estructura y dinmica del medio interestelar es la accin que los vientos de las estrellas de gran masa ejercen sobre el mismo. En este trabajo, mediante el uso de datos interferomtricos obtenidos en la banda de radio en la transicin de 21-cm del Hidrgeno neutro y de imgenes de la emisin de continuo en las bandas de 408 y 1420 MHz, de imgenes HIRES del satlite IRAS en 60 y 100 micrones, y de observaciones de continuo obtenidas con radiotelescopios de disco simple en 2695, 4850 y 8350 MHz se ha realizado un estudio multifrecuencia de los efectos que los vientos estelares de HD 192281, una estrella de tipo espectral O5 Vn((f))p, han tenido sobre el medio interestelar que rodea a la misma.

  6. Fotometra CCD en el campo del cmulo abierto Ruprecht 58

    NASA Astrophysics Data System (ADS)

    Giorgi, E. E.; Vzquez, R. A.; Seggewiss, W.; Ostrov, P.; Solivella, G. R.

    La regin de Vela-Puppis contiene varios cmulos cuyo estudio puede brindarnos informacin acerca de la cinemtica y el estado evolutivo de la poblacin estelar en las zonas externas del disco galctico. A travs de observaciones CCD en los filtros UBV en el campo del cmulo abierto Ruprecht 58 (?2000= 8h 14m 41s, ?2000=-31o 57'), se han determinado por primera vez los parmetros fundamentales de este cmulo tales como distancia, enrojecimiento y edad. Se analiza tambin la estructura de su secuencia principal y la presencia de gigantes y binarias.

  7. Cirugía de los trastornos del comportamiento: el estado del arte

    PubMed Central

    Yampolsky, Claudio; Bendersky, Damián

    2014-01-01

    Introducción: La cirugía de los trastornos del comportamiento (CTC) se está convirtiendo en un tratamiento más común desde el desarrollo de la neuromodulación. Métodos: Este artículo es una revisión no sistemática de la historia, indicaciones actuales, técnicas y blancos quirúrgicos de la CTC. Dividimos su historia en 3 eras: la primera comienza en los inicios de la psicocirugía y termina con el desarrollo de las tícnicas estereotácticas, cuando comienza la segunda era. Ésta se caracteriza por la realización de lesiones estereotácticas. Nos encontramos transitando la tercera era, que comienza cuando la estimulación cerebral profunda (ECP) comienza a ser usada en CTC. Resultados: A pesar de los errores graves cometidos en el pasado, hoy en día, la CTC está renaciendo. Los trastornos psiquiátricos que se más frecuentemente se tratan con cirugía son: depresión refractaria, trastorno obsesivo-compulsivo y síndrome de Tourette. Además, algunos pacientes con agresividad fueron tratados quirúrgicamente. Hay varios blancos estereotácticos descriptos para estos trastornos. La estimulación vagal puede ser usada también para depresión. Conclusión: Los resultados de la ECP en estos trastornos parecen alentadores. Sin embargo, se necesitan más estudios randomizados para establecer la efectividad de la CTC. Debe tenerse en cuenta que una apropiada selección de pacientes nos ayudará a realizar un procedimiento más seguro así como también a lograr mejores resultados quirúrgicos, conduciendo a la CTC a ser más aceptada por psiquiatras, pacientes y sus familias. Se necesita mayor investigación en varios temas como: fisiopatología de los trastornos del comportamiento, indicaciones de CTC y nuevos blancos quirúrgicos. PMID:25165612

  8. Screening of 185DelAG, 1014DelGT and 3889DelAG BRCA1 mutations in breast cancer patients from North-East India.

    PubMed

    Hansa, Jagadish; Kannan, Ravi; Ghosh, Sankar Kumar

    2012-01-01

    Around 1.35 million people of worldwide suffer from breast cancer each year, whereas in India, 1 in every 17 women develops the disease. Mutations of the Breast Cancer 1 (BRCA1) gene account for the majority of breast/ ovarian cancer families. The purpose of study was to provide a prevalence of BRCA1 germline mutations in the North-East Indian population. In relation to the personal and family history with the breast cancer, we found mutations in 6.25% and 12.5% respectively. Three mutations, 185DelAG, 1014DelGT and 3889DelAG, were observed in our North-East Indian patients in exons 2 and 11, resulting in truncation of the BRCA1 protein by forming stop codons individually at amino acid positions 39, 303 and 1265. Our results point to a necessity for an extensive mutation screening study of high risk breast cancer cases in our North-East Indian population, which will provide better decisive medical and surgical preventive options. PMID:23317271

  9. Interdisciplinary Unit: La Isla del Encanto (The Enchanted Island).

    ERIC Educational Resources Information Center

    Ford-Guerrera, Rebecca

    This document presents a series of 14 lesson plans in an interdisciplinary Spanish unit on "La isla del encanto/The Enchanted Island." The materials were prepared for students in grades 5 or 6 who have had basic Spanish instruction in previous grades. The students should also be familiar with basic concepts in English such as math computation, map

  10. 89. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    89. Paso Del Norte Hotel, 115-117 South El Paso St., detail view of roof, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  11. 87. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    87. Paso Del Norte Hotel, 115-117 South El Paso St., south and east facades, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  12. Faculty Activity Analysis in the Universidad Tecnica Del Estado Campuses.

    ERIC Educational Resources Information Center

    Karadima, Oscar

    An analysis of academic activities of college faculty at the eight campuses of Chile's Universidad Tecnica del Estado was conducted. Activities were grouped into seven categories: direct teaching, indirect teaching, research, community services, faculty development, academic administration, and other activities. Following the narrative

  13. del Nido cardioplegia in adult cardiac surgery - scopes and concerns.

    PubMed

    Valooran, George Jose; Nair, Shiv Kumar; Chandrasekharan, Krishnan; Simon, Rahul; Dominic, Cyril

    2016-01-01

    The convenience offered by a single-dose cardioplegia strategy is the avoidance of interruption of the flow of surgery and, more importantly, a significant reduction in the cross-clamp time. del Nido cardioplegia is an extracellular cardioplegic solution which serves these purposes and has been used successfully in pediatric cardiac surgery. The subsequent extrapolation of its use in adult cardiac surgery has returned encouraging results, as evidenced by recent literature. The use of del Nido cardioplegia in adults has been reported to shorten the cross-clamp time, cardiopulmonary bypass time and overall operating time. Intraoperative peak glucose value and insulin requirement have also been reported to be lower with del Nido cardioplegia, which can have prognostic significance. There have been reports of lesser incidence of atrial fibrillation and the number of defibrillations required with use of this technique. However, some unique concerns still remain. The lack of prospective randomized trials, the trend of elevation in CKMB (as reported by one study) and the need for a protocol-based approach to its use in adults are to be given due consideration while adopting its use in adults. This review attempts to have an overview on del Nido cardioplegia, its advantages, the recent studies comparing it with conventional cardioplegia techniques and the potential areas of concern with its use in adult cardiac surgery. PMID:26445810

  14. Design, aerodynamics and autonomy of the DelFly.

    PubMed

    de Croon, G C H E; Groen, M A; De Wagter, C; Remes, B; Ruijsink, R; van Oudheusden, B W

    2012-06-01

    One of the major challenges in robotics is to develop a fly-like robot that can autonomously fly around in unknown environments. In this paper, we discuss the current state of the DelFly project, in which we follow a top-down approach to ever smaller and more autonomous ornithopters. The presented findings concerning the design, aerodynamics and autonomy of the DelFly illustrate some of the properties of the top-down approach, which allows the identification and resolution of issues that also play a role at smaller scales. A parametric variation of the wing stiffener layout produced a 5% more power-efficient wing. An experimental aerodynamic investigation revealed that this could be associated with an improved stiffness of the wing, while further providing evidence of the vortex development during the flap cycle. The presented experiments resulted in an improvement in the generated lift, allowing the inclusion of a yaw rate gyro, pressure sensor and microcontroller onboard the DelFly. The autonomy of the DelFly is expanded by achieving (1) an improved turning logic to obtain better vision-based obstacle avoidance performance in environments with varying texture and (2) successful onboard height control based on the pressure sensor. PMID:22617112

  15. Cosmogenic Radionuclides in the Campo Del Cielo Iron Meteorite

    NASA Technical Reports Server (NTRS)

    Liberman, R. G.; FernandezNiello, J. O.; Reedy, R. C.; Fifield, L. K.; diTada, M. L.

    2001-01-01

    Cosmogenic Be-10, Al-26, Cl-36, Ca-41, and Ni-59 were measured in the Campo del Cielo iron meteorite. Our results led us to conclude that the pre-atmospheric radius might have been approximately 2 m. Comparisons with other big bodies are also presented. Additional information is contained in the original extended abstract.

  16. 78 FR 30268 - Del Norte County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... Forest Service Del Norte County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice... recommendations to the Forest Service concerning projects and funding consistent with the title II of the Act. The... Norte County Unified School District, Redwood Room, 301 West Washington Boulevard, Crescent City...

  17. 78 FR 50025 - Del Norte County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-16

    ... Forest Service Del Norte County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice... collaborative relationships and to provide advice and recommendations to the Forest Service concerning projects..., contact: Lynn Wright, RAC Committee Coordinator, Six Rivers National Forest, 707-441-3562,...

  18. 77 FR 47360 - Del Norte Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... Forest Service Del Norte Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of... recommendations to the Forest Service concerning projects and funding consistent with the title II of the Act. The... Unified School District, Redwood Room, 301 West Washington Boulevard, Crescent City CA 95531....

  19. Interdisciplinary Unit: La Isla del Encanto (The Enchanted Island).

    ERIC Educational Resources Information Center

    Ford-Guerrera, Rebecca

    This document presents a series of 14 lesson plans in an interdisciplinary Spanish unit on "La isla del encanto/The Enchanted Island." The materials were prepared for students in grades 5 or 6 who have had basic Spanish instruction in previous grades. The students should also be familiar with basic concepts in English such as math computation, map…

  20. Determinacion de Caracteristicas Opticas del Telescopio OAN150

    NASA Astrophysics Data System (ADS)

    Galan, M. J.; Cobos, F. J.

    1987-05-01

    En el Observatorio de Calar Alto, en Almera, Espaa, est ubicado un telescopio de 15O-cms de dimetro -construdo por REOSC- perteneciente al Observatorio Astronmico Nacional, con sede en Madrid, Espaa. La infraestructura tcnica del OAN ha sido tradicionalmente dbil y actualmente se est haciendo un esfuerzo por fortalecerla. Existe una informacin muy limitada del telescopio en general; de su ptica en particular se conocan los valores de los parmetros principales pero sin saber si stos corresponden a valores tericos de construccin. Por ello se consider necesario iniciar una investigacin para conocer en detalle los valores reales de las componentes pticas del telescopio, obtenindose algunos resultados de inters. El primario del telescopio OANl5O es aproximadamente F/3 y el siste ma en su conjunto es F/8.2, con su sistema corrector de campo. En trminos generales, la imagen es satisfactoria en todo el campo y, sin sistema corrector, la imagen axial tambin es buena. En un futuro muy cercano se piensa disear instrumentacin adicional para este telescopio. Conocer con mayor precisin sus caractersticas puede ser de gran utilidad para tal fin, pues se efectan los clculos considerando conjuntamente al telescopio y al instrumento.

  1. Síntesis: Resultados iniciales del Estudio Nacional de Ex

    Cancer.gov

    El 4 de noviembre de 2010, el Estudio Nacional de Exámenes de Pulmóndio a conocer resultados iniciales que indican que hubo 20% menos muertes por cáncer de pulmón entre los participantes del estudio evaluados con tomografía computarizada espiral de baja d

  2. Del Mod: A State-Wide System for School Improvement.

    ERIC Educational Resources Information Center

    Neale, Daniel C.

    This is a retrospective account of the Del Mod System, a statewide project to improve science and mathematics education in the elementary and secondary schools of Delaware. The project functioned from 1970-76 under the auspices of a committee including the presidents of institutions of higher education, the state superintendent of public

  3. Molculas orgnicas obtenidas en simulaciones experimentales del medio interestelar.

    NASA Astrophysics Data System (ADS)

    Muoz-Caro, Guillermo Manuel

    Las nubes moleculares son regiones de formacin de estrellas, con temperaturas cinticas entre 10-50 K y densidades de 103-106 tomos cm-3. Su materia est formada por gas y polvo interestelar. Estas partculas de polvo estn cubiertas por una fina capa de hielo, de unos 0.01 ?m, que contiene H2O y a menudo CO, CO2, CH3OH y NH3. El hielo es presumiblemente irradiado por fotones ultravioleta y rayos csmicos en las zonas poco profundas de las nubes moleculares y las regiones circunestelares. En un sistema de vaco, P 10-7 mbar, simulamos la deposicin de hielo a partir de 10 K y la irradiacin ultravioleta por medio de una lmpara de descarga de hidrgeno activada con microondas. La evolucin del hielo se observa por medio de un espectrmetro infrarrojo. De este modo es posible determinar la composicin del hielo observado en el medio interestelar y predecir la presencia de molculas an no detectadas en el espacio, que han sido producto del procesamiento del hielo en nuestros experimentos. Tambin es posible calentar el sistema hasta temperatura ambiente para sublimar el hielo depositado. Cuando el hielo ha sido previamente irradiado, se observa un residuo compuesto por molculas orgnicas complejas, algunas prebiticas, como varios cidos carboxlicos, aminas, amidas, steres y en menor proporcin molculas heterocclicas y aminocidos. Algunas de estas molculas podran detectarse en estado gaseoso por medio de observaciones milimtricas y de radio. Tambin podran estar presentes en el polvo cometario, cuyo anlisis qumico est planeado por las misiones Stardust y Rosetta. Mientras tanto, nuestro grupo est llevando a cabo el anlisis de partculas de polvo interplanetario (IDPs), algunas de las cuales pueden ser de origen cometario. Al igual que ocurre con los productos obtenidos por irradiacin del hielo en nuestros experimentos, algunas IDPs son ricas en material orgnico que contiene oxgeno.

  4. Molecular and cytogenetic characterization of del(9p) syndrome

    SciTech Connect

    Micale, M.A.; Haren, J.M.; Schwartz, S.

    1994-09-01

    The del(9p) syndrome is characterized by mental retardation, trigonocephaly, midface hypoplasia, upward slanting palpebral fissures, a long philtrum, and is typically associated with a breakpoint at 9p22. Seventeen unrelated patients with del(9p) and their parents were analyzed by high resolution chromosome studies, molecular analysis using a series of highly polymorphic dinucleotide repeat markers for 9p, and fluorescence in situ hybridization (FISH) to characterize precisely the extent of chromosome loss. Eleven patients were found to have an interstitial deletion with breakpoints assigned to 9p22.1 and 9p24.3 in most cases. Two patients had familial unbalanced translocations of maternal origin and four had de novo complex rearrangements identified by high resolution analysis and FISH utilizing chromosome libraries. PCR analysis of eleven families with eight minisatellite markers determined paternal origin of the deletion in six cases and maternal origin in five. This suggests that the molecular event resulting in the deletion is equally prevalent in both male and female gametogenesis and that genomic imprinting does not play a role in the pathogenesis of del(9p) syndrome. Increased precision in mapping these minisatellite markers was also possible through this work. This study demonstrates the existence of molecular heterogeneity as six patients with a cytological breakpoint at 9p22.1 revealed three different molecular breakpoints. These findings suggest that genotype-phenotype correlations in deletion syndromes such as this should be based on high-resolution molecular analysis of the deleted chromosome segment. Work is in progress to map a series of cosmid probes by FISH relative to chromosomal breakpoints in these patients. These combined studies should provide a better understanding of the mechanisms involved in the pathogenesis of del(9p) syndrome and may identify loci where genes responsible for one or more aspects of the del(9p) phenotype may reside.

  5. Extensión del Formalismo de Orbitales de Defecto Cuántico al tratamiento del efecto Stark (SQDO).

    NASA Astrophysics Data System (ADS)

    Menéndez, J. M.; Martín, I.; Velasco, A. M.

    El estudio experimental de las interacciones de átomos Rydberg altamente excitados con campos eléctricos ha experimentado un creciente interés durante las dos últimas décadas debido, en gran medida, al desarrollo de nuevas técnicas para crear y estudiar átomos Rydberg en el laboratorio. Acompañando a estas nuevas técnicas experimentales, es necesario el desarrollo de modelos teóricos que nos permitan contrastar sus medidas y conocer mejor los fundamentos de los mismos. Desde el punto de vista teórico el conocimiento del desdoblamiento de los niveles energéticos de un átomo en función de la magnitud del campo eléctrico aplicado (lo que se conoce como mapa Stark) es el mejor punto de partida para la descripción del sistema y un prerrequisito fundamental para el cálculo de distintas propiedades atómicas en presencia del campo eléctrico tales como intensidades de transición, umbrales de ionización de campo eléctrico, tiempos de vida, posición y anchura de cruces evitados, etc. En este trabajo presentamos la adaptación del método de orbitales de defecto cuántico [1,2,3] al tratamiento del efecto Stark (SQDO) [4] y su aplicación al cálculo de los desdoblamientos energéticos y fuerzas de oscilador de estados Rydberg en los átomos de Li, Na y K. El propósito de este estudio es, por un lado, desarrollar métodos fiables para la determinación de propiedades atómicas en presencia de campos eléctricos y, por otro, mostrar la fiabilidad de las funciones de onda QDO en la descripción del efecto Stark en sistemas atómicos.

  6. Analisis del contenido curricular de los Documentos Normativos del Programa de Ciencias en el area de biologia para la escuela superior del sistema de educacion publica de Puerto Rico: 1993-2012

    NASA Astrophysics Data System (ADS)

    Davila Montanez, Melissa

    Esta investigacion de naturaleza cualitativa se ocupo de realizar un analisis de contenido documental de los Documentos Normativos del Programa de Ciencias en el area de biologia de la escuela superior del sistema de educacion publica de Puerto Rico del periodo 1993-2012. Los documentos analizados fueron: Guia Curricular, 1995; Marco Curricular, 2003; Estandares de Excelencia, 1996, 2000 y Estandares de Contenido y Expectativas de Grado, 2007. Se indago si hubo cambios en significados en los Componentes Estructurales: Naturaleza de la ciencia, Paradigmas para la ensenanza de la ciencia, Funcion del curriculo formal, Mision de la ensenanza de la ciencia; Contenidos, destrezas y competencias, Estrategias de ensenanza y Evaluacion/Assessment del aprendizaje. El analisis sugiere que no hubo cambios sustanciales en los significados de los Componentes Estructurales. Los documentos estudiados muestran mayormente caracteristicas similares, aunque los documentos mas recientes eran mas descriptivos, explicativos y especificos.

  7. Genomic Copy Number Variations in the Myelodysplastic Syndrome and Acute Myeloid Leukemia Patients with del(5q) and/or -7/del(7q)

    PubMed Central

    Zhang, Rui; Kim, Young-Mi; Wang, Xianfu; Li, Yan; Lu, Xianglan; Sternenberger, Andrea R.; Li, Shibo; Lee, Ji-Yun

    2015-01-01

    The most common chromosomal abnormalities in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) are -5/del(5q) and -7/del(7q). When -5/del(5q) and -7/del(7q) coexist in patients, a poor prognosis is typically associated. Given that -5/del(5q) and/or -7/del(7q) often are accompanied with additional recurrent chromosomal alterations, genetic change(s) on the accompanying chromosome(s) other than chromosomes 5 and 7 may be important factor(s) affecting leukemogenesis and disease prognosis. Using an integrated analysis of karyotype, FISH and array CGH results in this study, we evaluated the smallest region of overlap (SRO) of chromosomes 5 and 7 as well as copy number alterations (CNAs) on the other chromosomes. Moreover, the relationship between the CNAs and del(5q) and -7/del(7q) was investigated by categorizing the cases into three groups based on the abnormalities of chromosomes 5 and 7 [group I: cases only with del(5q), group II: cases only with -7/del(7q) and group III: concurrent del(5q) and del(7q) cases]. The overlapping SRO of chromosome 5 from groups I and III was 5q31.1-33.1 and of chromosome 7 from groups II and III was 7q31.31-q36.1. A total of 318 CNAs were observed; ~ 78.3% of them were identified on chromosomes other than chromosomes 5 and 7, which were defined as 'other CNAs'. Group III was a distinctive group carrying the most high number (HN) CNAs, cryptic CNAs and 'other CNAs'. The loss of TP53 was highly associated with del(5q). The loss of ETV6 was specifically associated with group III. These CNAs or genes may play a secondary role in disease progression and should be further evaluated for their clinical significance and influence on therapeutic approaches in patients with MDS/AML carrying del(5q) and/or -7/del(7q) in large-scale, patient population study. PMID:26392809

  8. A model of Nevado del Ruiz Volcano, Colombia

    NASA Astrophysics Data System (ADS)

    Williams, Stanley N.; Meyer, Hansjrgen

    A workshop was held at the Observatorio Vulcanologico de Colombia in Manizales, Colombia, March 28 to April 1, 1988, to review the accumulated data on the disastrous November 13, 1985, eruption and related activity of Nevado del Ruiz volcano (Figure 1). Approximately 30 scientists from eight countries presented data, developed a preliminary model, and addressed the future problems of Ruiz. The volcano (see Figure 2) erupted just prior to the meeting, covering the meeting site with 1-2 mm of ash.The workshop was convened by Stanley N. Williams (Louisiana State University, Baton Rouge) and Hansjrgen Meyer (Universidad del Valle, Cali, Colombia). A special volume of the Journal of Volcanology and Geothermal Research is planned for the papers and letters presented at the workshop, with some additional contributions. Publication is expected in mid-1989.

  9. New records of fishes at Isla del Coco, Costa Rica

    USGS Publications Warehouse

    Garrison, V.H.

    1996-01-01

    Isla del Coco lies at 5 degrees 32'N latitude, 87 degrees 04'W longitude and is the sole peak of the Cocos Ridge exposed above sea level. This isolated island formed approximately 2 million years ago. It rises 575 m above the surface of the sea and covers 46 km2 (Castillo et aI., 1988). Five hundred km to the NNE is Costa Rica; 630 km SSW are the Galapagos Islands; 650 km to the E is Isla Malpelo, Colombia; and approximately 8,000 km W lie the Line Islands. Costa Rica claimed Isla del Coco in 1832 and declared it a National Park in 1978. The area of the park was increased to include the adjacent waters 5 km offshore in 1984 and 25 km offshore in 1991.

  10. Prognostic Impact of del(17p) and del(22q) as Assessed by Interphase FISH in Sporadic Colorectal Carcinomas

    PubMed Central

    Gonzlez-Gonzlez, Mara; Muoz-Bellvis, Lus; Mackintosh, Carlos; Fontanillo, Celia; Gutirrez, M. Laura; Abad, M. Mar; Bengoechea, Oscar; Teodosio, Cristina; Fonseca, Emilio; Fuentes, Manuel; De Las Rivas, Javier

    2012-01-01

    Background Most sporadic colorectal cancer (sCRC) deaths are caused by metastatic dissemination of the primary tumor. New advances in genetic profiling of sCRC suggest that the primary tumor may contain a cell population with metastatic potential. Here we compare the cytogenetic profile of primary tumors from liver metastatic versus non-metastatic sCRC. Methodology/Principal Findings We prospectively analyzed the frequency of numerical/structural abnormalities of chromosomes 1, 7, 8, 13, 14, 17, 18, 20, and 22 by iFISH in 58 sCRC patients: thirty-one non-metastatic (54%) vs. 27 metastatic (46%) disease. From a total of 18 probes, significant differences emerged only for the 17p11.2 and 22q11.2 chromosomal regions. Patients with liver metastatic sCRC showed an increased frequency of del(17p11.2) (10% vs. 67%;p<.001) and del(22q11.2) (0% vs. 22%;p?=?.02) versusnon-metastatic cases. Multivariate analysis of prognostic factors for overall survival (OS) showed that the only clinical and cytogenetic parameters that had an independent adverse impact on patient outcome were the presence of del(17p) with a 17p11.2 breakpoint and del(22q11.2). Based on these two cytogenetic variables, patients were classified into three groups: low- (no adverse features), intermediate- (one adverse feature) and high-risk (two adverse features)- with significantly different OS rates at 5-years (p<.001): 92%, 53% and 0%, respectively. Conclusions/Significance Our results unravel the potential implication of del(17p11.2) in sCRC patients with liver metastasis as this cytogenetic alteration appears to be intrinsically related to an increased metastatic potential and a poor outcome, providing additional prognostic information to that associated with other cytogenetic alterations such as del(22q11.2). Additional prospective studies in larger series of patients would be required to confirm the clinical utility of the new prognostic markers identified. PMID:22912721

  11. Solar adaptive optics at the Observatorio del Teide, Tenerife

    NASA Astrophysics Data System (ADS)

    Soltau, Dirk; Berkefeld, Thomas; Schmidt, Dirk; von der Lühe, Oskar

    2013-10-01

    Observing the Sun with high angular resolution is difficult because the turbulence in the atmosphere is strongest during day time. In this paper we describe the principles of solar adaptive optics exemplified by the two German solar telescopes VTT and GREGOR at the Observatorio del Teide. With theses systems we obtain near diffraction limited images of the Sun. Ways to overcome the limits of conventional AO by applying multiconjugate adaptive optics (MCAO) are shown.

  12. Localizador de Servicios de Apoyo - Instituto Nacional del Cncer

    Cancer.gov

    HealthCare.gov es el primer centro de datos de alternativas de cobertura mdica del Departamento de Salud y Servicios Humanos de EE. UU. que combina los programas pblicos (ej. Medicare, Medicaid) con la informacin de ms de 1 000 planes de seguros privados. El usuario puede buscar en Internet las opciones de cobertura mdica especficas a su situacin y a su comunidad local. Igualmente, Healthcare.gov tiene informacin sobre la reforma sanitaria Affordable Care Act.

  13. Po del Ro Hortega and the discovery of the oligodendrocytes.

    PubMed

    Prez-Cerd, Fernando; Snchez-Gmez, Mara Victoria; Matute, Carlos

    2015-01-01

    Po del Ro Hortega (1882-1945) discovered microglia and oligodendrocytes (OLGs), and after Ramn y Cajal, was the most prominent figure of the Spanish school of neurology. He began his scientific career with Nicols Achcarro from whom he learned the use of metallic impregnation techniques suitable to study non-neuronal cells. Later on, he joined Cajal's laboratory. and Subsequently, he created his own group, where he continued to develop other innovative modifications of silver staining methods that revolutionized the study of glial cells a century ago. He was also interested in neuropathology and became a leading authority on Central Nervous System (CNS) tumors. In parallel to this clinical activity, del Ro Hortega rendered the first systematic description of a major polymorphism present in a subtype of macroglial cells that he named as oligodendroglia and later OLGs. He established their ectodermal origin and suggested that they built the myelin sheath of CNS axons, just as Schwann cells did in the periphery. Notably, he also suggested the trophic role of OLGs for neuronal functionality, an idea that has been substantiated in the last few years. Del Ro Hortega became internationally recognized and established an important neurohistological school with outstanding pupils from Spain and abroad, which nearly disappeared after his exile due to the Spanish civil war. Yet, the difficulty of metal impregnation methods and their variability in results, delayed for some decades the confirmation of his great insights into oligodendrocyte biology until the development of electron microscopy and immunohistochemistry. This review aims at summarizing the pioneer and essential contributions of del Ro Hortega to the current knowledge of oligodendrocyte structure and function, and to provide a hint of the scientific personality of this extraordinary and insufficiently recognized man. PMID:26217196

  14. Descripcin del corongrafo a ser instalado en Argentina (MICA)

    NASA Astrophysics Data System (ADS)

    Stenborg, G.; Francile, C.; Schwenn, R.; Epple, A.; Rovira, M.

    El ``Corongrafo de espejo para Argentina'' es un telescopio solar terrestre a ser colocado en el Observatorio Astronmico Flix Aguilar (El Leoncito), antes de finalizar 1996, como parte de un programa de ciencia bilateral entre Alemania y Argentina. Eclipses fotogrficos de alta resolucin han revelado que la corona solar es altamente estructurada y variable. De hecho, est contnuamente deformada y moldeada por los movimientos convectivos de los extremos de los arcos magnticos en la fotosfera, estando, en muchas oportunidades, afectada por explosivas liberaciones de energa. MICA, en conjuncin con otros telescopios solares espaciales y terrestres, tratar de contribuir al entendimiento de cuestiones fundamentales de la fsica solar. Entre ellas: cmo la corona est siendo calentada, dnde y cmo el viento solar es acelerado, qu causa los transitorios coronales, etc. Para ello investigar la distribucin de los parmetros del plasma y su evolucin con el tiempo, la estructura espacial de la corona en fina y gran escala, procesos que ocurren en los transitorios coronales y factores que los disparan, etc. Para responder a estas cuestiones MICA observar la atmsfera solar por sobre el limbo entre 1.1 y 2 radios solares aproximadamente, usando un nuevo tipo de sistema coronogrfico que permite suprimir el brillo del disco solar suficientemente bien, tomando las imgenes con una cmara CCD de 1024x1024 pixels, codificada en 12 bits, pudiendo el mismo ser operado en forma remota. En la presente exposicin describiremos las caractersticas del instrumento, cmo ser controlado y qu esperamos observar basados en las imgenes obtenidas por los telescopios de similares caractersticas LASCO C1 a bordo del SOHO y PICO (ubicado en el Observatorio de Pic du Midi, Francia).

  15. 3. 1939 DROP STRUCTURE IN DEL MAR PARK SURMOUNTED BY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. 1939 DROP STRUCTURE IN DEL MAR PARK SURMOUNTED BY RECENT PEDESTRIAN BRIDGE. - Highline Canal, Sand Creek Lateral, Beginning at intersection of Peoria Street & Highline Canal in Arapahoe County (City of Aurora), Sand Creek lateral Extends 15 miles Northerly through Araphoe County, City & County of Denver, & Adams County to its end point, approximately 1/4 mile Southest of intersectioin of D Street & Ninth Avenue in Adams County (Rocky Mountain Arsenal, Commerce City Vicinity), Commerce City, Adams County, CO

  16. 2. COURSE OF THE LATERAL THROUGH DEL NORTHWEST MAR PARK. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. COURSE OF THE LATERAL THROUGH DEL NORTHWEST MAR PARK. - Highline Canal, Sand Creek Lateral, Beginning at intersection of Peoria Street & Highline Canal in Arapahoe County (City of Aurora), Sand Creek lateral Extends 15 miles Northerly through Araphoe County, City & County of Denver, & Adams County to its end point, approximately 1/4 mile Southest of intersectioin of D Street & Ninth Avenue in Adams County (Rocky Mountain Arsenal, Commerce City Vicinity), Commerce City, Adams County, CO

  17. Modeling Visibility in the EL Paso del Norte Region

    NASA Astrophysics Data System (ADS)

    Fitzgerald, R. M.; Medina, R.; DuBois, D. W.; Novlan, D.

    2013-12-01

    Poor visibility is a subject of growing public concern throughout the U.S, and an active area of research. Its societal impacts in air quality, aviation transport and traffic are significant. Aerosols play a fundamental role in the attenuation of solar radiation, and also affect visibility. The scattering and extinction coefficients of aerosol particles in the Paso del Norte Region have been calculated using the T- matrix model in conjunction with a laser particle counter. Inter-comparison of the model's results of the scattering and absorption coefficients against the corresponding data from a Photoacustic Extinctiometer instrument (which measures in-situ absorption and scattering coefficients of aerosol particles) shows excellent agreement. In addition, the volume-weighted method is used to determine the composite index of refraction which is representative of the aerosols for the Paso del Norte Airshed to obtain information of the type of aerosol particles present in the Airshed. The Single Scattering Albedo has also been retrieved using our methodology to obtain further insight into the type of aerosols present on a given day. Finally, the Koschmieder equation has been used to calculate the visual range or visibility, and was correlated with the PM2.5 and PM10 particle concentration present in the Airshed. Our methodology will allow a better understanding of the size and type of aerosol particles that are most detrimental to the visibility for the Paso del Norte Region.

  18. Modeling visibility in the Paso del Norte (PDN) Region

    NASA Astrophysics Data System (ADS)

    Medina Calderon, Richard

    Poor visibility is a subject of growing public concern throughout the U.S, and an active area of research. Its societal impacts on air quality, aviation transport and traffic are significant. Aerosols play a fundamental role in the attenuation of solar radiation, and also affect visibility. The scattering and extinction coefficients of aerosol particles in the Paso del Norte Region have been calculated using the T- matrix model in conjunction with a laser particle counter. Inter-comparison of the model's results of the scattering and absorption coefficients against the corresponding data from a Photoacustic extinctiometer instrument (which measures in-situ absorption and scattering coefficients of aerosol particles) shows excellent agreement. In addition, the volume-weighted method is used to determine the composite index of refraction which is representative of the aerosols for the Paso del Norte Region to obtain information of the type of aerosol particles present in the Region. The Single Scattering Albedo has also been retrieved using this methodology to obtain further insight into the type of aerosols present on a given day. Finally, the Koschmieder equation has been used to calculate the visual range or visibility, and was correlated with the PM2.5 and PM10 particle concentration present in the Region. Our methodology will allow a better understanding of the size and type of aerosol particles that are most detrimental to the visibility for the Paso Del Norte Region.

  19. Los plaguicidas y la contaminacion del medio ambiente Venezolano

    USGS Publications Warehouse

    Stickel, L.F.; Stickel, W.H.

    1972-01-01

    RESUMEN DE RECOMENDACIONES Recomendaciones para el Programa de Investigacion: 1. Establecer un sistema de muestreo biologico para detectar los niveles tendencias de los productos quimicos toxicos en un peque?o numero de si tios representativos. 2. Mantener continua vigilancia de la contaminacion ambiental, mediante la seleccion acertadamente dirigida de las zonas afectadas y de las fuentes de contaminacion. 3. Realizar estudios acerca de las poblaciones de animales silvestres, y del exito de los procesos reproductivos de las especies o grupos clayes de animales que se consideran mas gravemente afectados. 4. Preparar recomendaciones para una accion gubernamental de proteccion al hombre, a la fauna silvestre y al medio ambiente. Recomendaciones para la Accion Administrativa: 1. Establecer limites a la tolerancia de los residuos de plaguicidas en los alimentos. Constituye una medida clave para disminuir la contaminacion ambiental. 2. Establecer normas de calidad del agua para las corrientes, represas, la gos y otros cuerpos. Es la segunda medida clave para reducir la contaminacion del ambiente 3. Exigir un tratamiento adecuado de los efluentes industriales, especialmente antes de que se construyan las nuevas plantas. 4. Exigir a los agricultores que en el uso de plaguicidas sigan los consejos tecnicos autorizados y negar a los vendedores el derecho a recomendar productos por su cuenta. 5. Tomar medidas para recoger y eliminar los recipientes y sobrantes de los plaguicidas.

  20. Nuevo sitio web en espaol del Instituto Nacional del Cncer, (NCI, por sus siglas en ingls) Cancer.gov en espaol - Silvia Inz Salazar - transcript

    Cancer.gov

    Transmisiones de radio para promover Cancer.gov en espa%XF1ol | Nuevo sitio web en espa%XF1ol del Instituto Nacional del C%XE1ncer, (NCI, por sus siglas en ingl%XE9s) Cancer.gov en espa%XF1ol | Transcripci%XF3n Transmisiones de radio para promover

  1. The 2012 Reactivation of Nevado del Ruiz Volcano, Colombia

    NASA Astrophysics Data System (ADS)

    Ordoñez, M.; Lopez, C.; Cortes, G. P.; Londoño, J.; Battaglia, M.

    2012-12-01

    Nevado Del Ruiz volcano is most known for its tragic November 13, 1985 explosive event, when a small eruption produced an enormous lahar that buried and destroyed the town of Armero in Tolima, causing an estimated 25,000 deaths. Similar but less deadly incidents occurred in 1595 and 1845, again where a small explosive eruption was followed by a large lahar. Other minor explosive events occurred in 1805, 1828-9, and 1989. The Armero tragedy was a major factor in the establishment of a volcano monitoring program in Colombia. The network monitoring Nevado del Ruiz volcano has dramatically improved since the 1985 eruption and now includes geodetic, geophysical, geochemical and visual monitoring. The activity of Nevado Del Ruiz between 1985 and 1991 saw significant increases in seismicity and significant gas emissions with the main eruptive events of November 13, 1985 and September 1st, 1989. The volcano became seismically active again in 1995 and 2002, whit an increase in seismicity, without any gas emission or eruption. In October 2010 the volcano entered in a new phase of unrest with increased seismicity (HB events), deformation and gas emissions, likely associated with a new magma intrusion. In February, 2012 the volcano had a new and significant increase in seismicity (VT-LP), followed in May and June 2012 by small phreatic and phreato-magmatic eruption. This eruptive activity is still ongoing. Deformation measurements at Nevado Del Ruiz volcano have been carried out since just after the tragic eruption of the 13th of November 1985. In 2007 seven new electronic tilt meters were set up to monitor the deformation. Significant uplift was measured from August 2007 to October 2010, for a total cumulative value of more than 500 microradians. From the end of 2011 to July 2012, moderate changes of uplift were detected for a total of 70 microradians. The analysis of these deformation data shows a pattern of surface displacement associated with an intrusion of new magma coming from a reservoir located approximately at a depth of approximately 8 km and accumulating in shallow reservoir less than 2 km beneath the main crater. We fit the data with both a spherical and a vertical spheroid source. The spherical source indicates the existence of an intrusion at a depth of 1823±1056 m with a volume change of (7±17)×106 m3 . The vertical spheroidal source suggests a source 1855 m deep with a volume change of 6×106 m3 . These results imply that while we have strong bounds on the depth of the intrusion, the actual volume of the source is less certain. Gas emission from Nevado del Ruiz volcano in April 7, 2012.

  2. Estrategia innovadora enfocada en parejas del mismo sexo para disminuir la infección del VIH en hombres Latinos

    PubMed Central

    Martinez, Omar; Wu, Elwin; Sandfort, Theo; Shultz, Andrew Z.; Capote, Jonathan; Chávez, Silvia; Moya, Eva; Dodge, Brian; Morales, Gabriel; Porras, Antonio; Ovejero, Hugo

    2014-01-01

    Resumen El VIH es un problema de salud importante dentro de la comunidad latina de los Estados Unidos. Gracias a los esfuerzos de prevención, los niveles de contagio entre los latinos se han mantenido estables por más de una década. Sin embargo, esta población sigue siendo afectada a niveles muy altos, en particular entre hombres que tienen sexo con hombres (HSH), de origen latino y que hablan principalmente el idioma español. Existen varios factores que contribuyen a la transmisión del VIH entre esta población, como son: el uso de drogas; la violencia dentro de la pareja; la presencia de infecciones de transmisión sexual; relaciones sexuales sin protección, dentro y fuera de la pareja; el evadir la búsqueda de recursos (prueba y tratamiento adecuado) por temor a ser discriminado o por su estatus migratorio; la escasez de recursos económicos o estado de pobreza y los patrones relacionados a la migración. En particular, Investigaciones Epidemiológicas de Comportamientos han determinado: cómo algunas dinámicas en parejas están directamente asociadas a los comportamientos sexuales de riesgos. En consecuencia, es necesaria mayor investigación para identificar esas dinámicas, y a su vez, realizar intervenciones dirigidas a la reducción de conductas de riesgo enfocadas en parejas de hombres del mismo sexo. En este escrito, se describe la importancia del uso de las relaciones de pareja como estrategia en la reducción de la trasmisión del VIH/SIDA en HSH de origen latino y que hablan principalmente el idioma español en los Estados Unidos. PMID:25580466

  3. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants

    PubMed Central

    2011-01-01

    Background Indian medicinal plants used in the Ayurvedic traditional system to treat diabetes are a valuable source of novel anti-diabetic agents. Pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post-prandial hyperglycemia via control of starch breakdown. In this study, seventeen Indian medicinal plants with known hypoglycemic properties were subjected to sequential solvent extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory potential on PPA (porcine pancreatic α-amylase). Preliminary phytochemical analysis of the lead extracts was performed in order to determine the probable constituents. Methods Analysis of the 126 extracts, obtained from 17 plants (Aloe vera (L.) Burm.f., Adansonia digitata L., Allium sativum L., Casia fistula L., Catharanthus roseus (L.) G. Don., Cinnamomum verum Persl., Coccinia grandis (L.) Voigt., Linum usitatisumum L., Mangifera indica L., Morus alba L., Nerium oleander L., Ocimum tenuiflorum L., Piper nigrum L., Terminalia chebula Retz., Tinospora cordifolia (Willd.) Miers., Trigonella foenum-graceum L., Zingiber officinale Rosc.) for PPA inhibition was initially performed qualitatively by starch-iodine colour assay. The lead extracts were further quantified with respect to PPA inhibition using the chromogenic DNSA (3, 5-dinitrosalicylic acid) method. Phytochemical constituents of the extracts exhibiting≥ 50% inhibition were analysed qualitatively as well as by GC-MS (Gas chromatography-Mass spectrometry). Results Of the 126 extracts obtained from 17 plants, 17 extracts exhibited PPA inhibitory potential to varying degrees (10%-60.5%) while 4 extracts showed low inhibition (< 10%). However, strong porcine pancreatic amylase inhibitory activity (> 50%) was obtained with 3 isopropanol extracts. All these 3 extracts exhibited concentration dependent inhibition with IC50 values, viz., seeds of Linum usitatisumum (540 μgml-1), leaves of Morus alba (1440 μgml-1) and Ocimum tenuiflorum (8.9 μgml-1). Acarbose as the standard inhibitor exhibited an IC50 (half maximal inhibitory concentration)value of 10.2 μgml-1. Phytochemical analysis revealed the presence of alkaloids, tannins, cardiac glycosides, flavonoids, saponins and steroids with the major phytoconstituents being identified by GC-MS. Conclusions This study endorses the use of these plants for further studies to determine their potential for type 2 diabetes management. Results suggests that extracts of Linum usitatisumum, Morus alba and Ocimum tenuiflorum act effectively as PPA inhibitors leading to a reduction in starch hydrolysis and hence eventually to lowered glucose levels. PMID:21251279

  4. Fab antibody fragments: some applications in clinical toxicology.

    PubMed

    Flanagan, Robert J; Jones, Alison L

    2004-01-01

    This review provides current information on the use of antigen-binding fragments (Fab) from cleaved antibodies to treat poisoning with digoxin and other potent, low formula mass poisons, such as colchicine and tricyclic antidepressants. Anti-digoxin Fab fragments have been used successfully for many years in the management of severe poisoning with digoxin, digitoxin, and a range of other structurally related compounds, including cardiotoxins from Nerium and Thevetia sp. (oleander) and Bufo sp. (toads). However, their main use remains treating digoxin poisoning. Equimolar doses of anti-digoxin Fab fragments completely bind digoxin in vivo. The approximate dose of Fab fragments (mg) is 80 times the digoxin body burden (mg). If neither the dose ingested nor the plasma digoxin/digitoxin concentration is known, in an adult 380 mg of anti-digoxin Fab fragments should be given. The dose for elderly patients or those with renal impairment should be similar to that for those with normal renal function. Fab fragments have a plasma half-life of 12-20 hours, but this can be prolonged in patients with renal impairment. Analysis of serum ultrafiltrate using an immunoassay shown not to have matrix bias remains the most accurate approach to measuring free digoxin in the presence of anti-digoxin Fab fragments. The antibody fragments are given intravenously over 15-30 minutes after dilution to at least 250 mL with plasma protein solution, 0.9% (w/v) sodium chloride, or deionised water, except in infants where the volume infused can be reduced. Factors limiting the efficacy of Fab fragments are the dose, the duration of the infusion and any delay in administration. Guidelines for Fab fragment administration in children include (i) dilution to a final Fab concentration of 10 g/L in either 5% (w/v) dextrose or 0.9% (w/v) sodium chloride; (ii) infusion through a 0.22 microm filter; (iii) administration of the total dose over a minimum of 30 minutes; and (iv) avoiding coadministration of other drugs and/or electrolyte solutions. Fab fragments are generally well tolerated. Adverse effects attributable to Fab treatment include hypokalaemia and exacerbation of congestive cardiac failure; renal function could be impaired in some patients. Fab fragment preparations for treating acute colchicine and tricyclic antidepressant poisoning have been developed, but are not available commercially. Colchicine poisoning is rare in Western countries, and pharmacological management together with supportive care is usually effective even in severe tricyclic antidepressant overdosage. Attempts have been made to produce anti-paraquat antibodies capable of enhancing paraquat elimination from the lung, but thus far all such attempts have proved unsuccessful. PMID:15554746

  5. Effect of medicinal and aromatic plants on rumen fermentation, protozoa population and methanogenesis in vitro.

    PubMed

    Bhatta, R; Baruah, L; Saravanan, M; Suresh, K P; Sampath, K T

    2013-06-01

    The potential of tannins from 21 medicinal and aromatic plant leaves as antimethanogenic additives in ruminant feeds was investigated. The effect of tannin from these leaves on rumen fermentation parameters, protozoa population and methanogenesis was studied by incubating the samples [200?mg dry matter (DM)] without and with polyethylene glycol (PEG)-6000 (400?mg DM) as a tannin binder during 24-h incubation in the in vitro Hohenheim gas method. Based on the methane percentage estimated in the total gas produced, methane production in millilitre was calculated [methane volume (ml)?=?methane %??total gas produced (ml) in 24?h]. In the samples, crude protein and neutral detergent fibre (g/kg DM) ranged from 113 to 172 and from 352 to 444 respectively. The total phenol (TP; g/kg DM) content was highest in Terminalia chebula (274) followed by Hemigraphis colorata (71) and Sapindus laurifolia (51) respectively. In the remaining samples, it was <43?g/kg DM. Activity of tannins, as represented by the increase in gas volume on addition of PEG, ranged from 0 to 133%, with the highest being recorded in T.chebula. The per cent increase in methane on PEG addition was 0 for Ammi majus, Aristolochia indica, Cascabela thevetia, Ipomea nil and Lantana camara, illustrating that tannins present in these samples had no effect on methane concentration. The PEG addition increased the total protozoa count by >50% in A.indica and C. thevetica. One of the important findings of our study was that of the 21 samples screened, Entodinia population increased in 12 with PEG as compared to 7 where Holotricha increased, indicating higher susceptibility of Entodinia to tannin. There was no increase in the protozoa population with PEG when incubating Cardiospermum halicacabum, Clerodendrum inerme, Dioscorea floribunda, Nerium oleander and Selastras paniculatus, which strongly suggested that methane suppression recorded in these samples was not because of a defaunating effect of their tannins per se. The fermentation pattern reflected increased total volatile fatty acid (TVFA) concentration from 0 to 28.3% with PEG addition among the leaves. Our results confirmed further observations that methanogenesis in vitro is not essentially related to density of protozoa population. Secondly, medicinal and aromatic plants such as C.inerme, Gymnema sylvestre and Sapindus laurifolia containing tannins appear to have a potential to suppress in vitro methanogenesis. PMID:22385477

  6. Cytotoxic effects of oosporein isolated from endophytic fungus Cochliobolus kusanoi

    PubMed Central

    Ramesha, Alurappa; Venkataramana, M.; Nirmaladevi, Dhamodaran; Gupta, Vijai K.; Chandranayaka, S.; Srinivas, Chowdappa

    2015-01-01

    In the present study, oosporein, a fungal toxic secondary metabolite known to be a toxic agent causing chronic disorders in animals, was isolated from fungus Cochliobolus kusanoi of Nerium oleander L. Toxic effects of oosporein and the possible mechanisms of cytotoxicity as well as the role of oxidative stress in cytotoxicity to Madin-Darby canine kidney kidney cells and RAW 264.7 splene cells were evaluated in vitro. Also to know the possible in vivo toxic effects of oosporein on kidney and spleen, Balb/C mouse were treated with different concentrations of oosporein ranging from 20 to 200 μM). After 24 h of exposure histopathological observations were made to know the effects of oosporein on target organs. Oosporein induced elevated levels of reactive oxygen species (ROS) generation and high levels of malondialdehyde, loss of mitochondrial membrane potential, induced glutathione hydroxylase (GSH) production was observed in a dose depended manner. Effects oosporein on chromosomal DNA damage was assessed by Comet assay, and increase in DNA damage were observed in both the studied cell lines by increasing the oosporein concentration. Further, oosporein treatment to studied cell lines indicated significant suppression of oxidative stress related gene (Superoxide dismutase1 and Catalase ) expression, and increased levels of mRNA expression in apoptosis or oxidative stress inducing genes HSP70, Caspase3, Caspase6, and Caspase9 as measured by quantitative real time-PCR assay. Histopathological examination of oosporein treated mouse kidney and splenocytes further revealed that, oosporein treated target mouse tissues were significantly damaged with that of untreated sam control mice and these effects were in directly proportional to the the toxin dose. Results of the present study reveals that, ROS is the principle event prompting increased oosporein toxicity in studied in vivio and in vitro animal models. The high previlance of these fungi in temperate climates further warrants the need of safe food grain storage and processing practices to control the toxic effects of oosporein to humans and live stock. PMID:26388840

  7. Fungal Planet description sheets: 214-280.

    PubMed

    Crous, P W; Shivas, R G; Quaedvlieg, W; van der Bank, M; Zhang, Y; Summerell, B A; Guarro, J; Wingfield, M J; Wood, A R; Alfenas, A C; Braun, U; Cano-Lira, J F; García, D; Marin-Felix, Y; Alvarado, P; Andrade, J P; Armengol, J; Assefa, A; den Breeÿen, A; Camele, I; Cheewangkoon, R; De Souza, J T; Duong, T A; Esteve-Raventós, F; Fournier, J; Frisullo, S; García-Jiménez, J; Gardiennet, A; Gené, J; Hernández-Restrepo, M; Hirooka, Y; Hospenthal, D R; King, A; Lechat, C; Lombard, L; Mang, S M; Marbach, P A S; Marincowitz, S; Marin-Felix, Y; Montaño-Mata, N J; Moreno, G; Perez, C A; Pérez Sierra, A M; Robertson, J L; Roux, J; Rubio, E; Schumacher, R K; Stchigel, A M; Sutton, D A; Tan, Y P; Thompson, E H; van der Linde, E; Walker, A K; Walker, D M; Wickes, B L; Wong, P T W; Groenewald, J Z

    2014-06-01

    Novel species of microfungi described in the present study include the following from South Africa: Cercosporella dolichandrae from Dolichandra unguiscati, Seiridium podocarpi from Podocarpus latifolius, Pseudocercospora parapseudarthriae from Pseudarthria hookeri, Neodevriesia coryneliae from Corynelia uberata on leaves of Afrocarpus falcatus, Ramichloridium eucleae from Euclea undulata and Stachybotrys aloeticola from Aloe sp. (South Africa), as novel member of the Stachybotriaceae fam. nov. Several species were also described from Zambia, and these include Chaetomella zambiensis on unknown Fabaceae, Schizoparme pseudogranati from Terminalia stuhlmannii, Diaporthe isoberliniae from Isoberlinia angolensis, Peyronellaea combreti from Combretum mossambiciensis, Zasmidium rothmanniae and Phaeococcomyces rothmanniae from Rothmannia engleriana, Diaporthe vangueriae from Vangueria infausta and Diaporthe parapterocarpi from Pterocarpus brenanii. Novel species from the Netherlands include: Stagonospora trichophoricola, Keissleriella trichophoricola and Dinemasporium trichophoricola from Trichophorum cespitosum, Phaeosphaeria poae, Keissleriella poagena, Phaeosphaeria poagena, Parastagonospora poagena and Pyrenochaetopsis poae from Poa sp., Septoriella oudemansii from Phragmites australis and Dendryphion europaeum from Hedera helix (Germany) and Heracleum sphondylium (the Netherlands). Novel species from Australia include: Anungitea eucalyptorum from Eucalyptus leaf litter, Beltraniopsis neolitseae and Acrodontium neolitseae from Neolitsea australiensis, Beltraniella endiandrae from Endiandra introrsa, Phaeophleospora parsoniae from Parsonia straminea, Penicillifer martinii from Cynodon dactylon, Ochroconis macrozamiae from Macrozamia leaf litter, Triposporium cycadicola, Circinotrichum cycadis, Cladosporium cycadicola and Acrocalymma cycadis from Cycas spp. Furthermore, Vermiculariopsiella dichapetali is described from Dichapetalum rhodesicum (Botswana), Ophiognomonia acadiensis from Picea rubens (Canada), Setophoma vernoniae from Vernonia polyanthes and Penicillium restingae from soil (Brazil), Pseudolachnella guaviyunis from Myrcianthes pungens (Uruguay) and Pseudocercospora neriicola from Nerium oleander (Italy). Novelties from Spain include: Dendryphiella eucalyptorum from Eucalyptus globulus, Conioscypha minutispora from dead wood, Diplogelasinospora moalensis and Pseudoneurospora canariensis from soil and Inocybe lanatopurpurea from reforested woodland of Pinus spp. Novelties from France include: Kellermania triseptata from Agave angustifolia, Zetiasplozna acaciae from Acacia melanoxylon, Pyrenochaeta pinicola from Pinus sp. and Pseudonectria rusci from Ruscus aculeatus. New species from China include: Dematiocladium celtidicola from Celtis bungeana, Beltrania pseudorhombica, Chaetopsina beijingensis and Toxicocladosporium pini from Pinus spp. and Setophaeosphaeria badalingensis from Hemerocallis fulva. Novel genera of Ascomycetes include Alfaria from Cyperus esculentus (Spain), Rinaldiella from a contaminated human lesion (Georgia), Hyalocladosporiella from Tectona grandis (Brazil), Pseudoacremonium from Saccharum spontaneum and Melnikomyces from leaf litter (Vietnam), Annellosympodiella from Juniperus procera (Ethiopia), Neoceratosperma from Eucalyptus leaves (Thailand), Ramopenidiella from Cycas calcicola (Australia), Cephalotrichiella from air in the Netherlands, Neocamarosporium from Mesembryanthemum sp. and Acervuloseptoria from Ziziphus mucronata (South Africa) and Setophaeosphaeria from Hemerocallis fulva (China). Several novel combinations are also introduced, namely for Phaeosphaeria setosa as Setophaeosphaeria setosa, Phoma heteroderae as Peyronellaea heteroderae and Phyllosticta maydis as Peyronellaea maydis. Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa. PMID:25264390

  8. Contribucin al flujo infrarrojo de las estrellas Be de la recombinacin dielectrnica del MgII

    NASA Astrophysics Data System (ADS)

    Cruzado, A.; di Rocco, H.; Ringuelet, A.

    Para evaluar la contribucin del proceso de recombinacin dielectrnica del tomo de MgII al exceso de flujo infrarrojo observado en las estrellas Be, calculamos la energa emitida en las lneas originadas por este proceso. Se evaluaron los efectos de las condiciones fsicas del medio, como la temperatura electrnica y la densidad electrnica, sobre el flujo emitido. Se consider tambin la influencia de una posible opacidad.

  9. Screening for 185delAG in the Ashkenazim.

    PubMed Central

    Richards, C S; Ward, P A; Roa, B B; Friedman, L C; Boyd, A A; Kuenzli, G; Dunn, J K; Plon, S E

    1997-01-01

    A study was initiated to assess interest, educational effectiveness, and implications of genetic testing for the common BRCA1 mutation, 185delAG, in the Ashkenazim. Of 333 individuals who attended group sessions, 309 (92%) participated in the study. Participants were categorized as having negative family history (67%), positive family history (defined, by a relaxed criterion, as one first-degree relative or two second-degree relatives with breast [premenopausal] or ovarian cancer) (22%), positive personal history (7%), and both positive personal history and positive family history (4%). Group education was effective, as shown by the improvement in participant scores from pre- to posteducation tests. For the 289 individuals (94%) who requested testing, the major reasons included concern for their own risk, concern for the risk of their children, and desire to learn about surveillance options. The most common reason given by participants who declined testing was concern about health insurance. Six participants found to be heterozygous for the 185delAG mutation received results and were offered genetic counseling. Participants had consented for additional testing without receiving results and were screened for the 6174delT mutation in BRCA2, and seven were found to be positive. All identified carriers reported at least one first- or second-degree relative with a history of breast or ovarian cancer, although they did not all meet our study criteria for positive family history. Given these outcomes, we conclude that screening for breast and ovarian cancer susceptibility is most appropriate for individuals with a positive personal or positive family cancer history. We propose a guideline for future studies designed to identify individuals who may benefit from genetic testing for inherited breast and ovarian cancer. Images Figure 1 PMID:9150156

  10. Optical photometry and spectroscopy of Nova Del 2013

    NASA Astrophysics Data System (ADS)

    Tomov, T.; Ilkiewicz, K.; Swierczynski, E.; Belcheva, M.; Dimitrov, D.

    2013-08-01

    We report optical BV photometry and spectroscopy of Nova Del 2013, carried out between August 14.88 UT and August 15.08 UT. Using a 60 cm Cassegrain telescope at the Nicolaus Copernicus University Observatory (Torun, Poland) we estimated the V brightness of the Nova to be 6.31+/-0.02 mag and 6.18+/-0.03 mag on Aug. 14.94 UT and Aug. 15.02 UT respectively. HD 194113 (F2, V=8.00 mag) was used as a comparison star.

  11. Visual and Near-IR Photometry of Nova Del 2013

    NASA Astrophysics Data System (ADS)

    Gehrz, R. D.; Dykhoff, D. A.; Shenoy, D. P.

    2013-08-01

    We report Visual and Near-IR photometry of Nova Del 2013 taken at the University of Minnesota's O'Brien Observatory (Marine-on-St-Croix, Minnesota, USA). VRIJHKLM photometry were obtained on two successive nights using an AsSi bolometer. Vega (alpha Lyrae) was used as the standard star. Our photometry show: August 16.2 UT: V = 4.8 +/- 0.1, R = 5.4 +/- 0.1, I = 5.2 +/- 0.1, J = 4.4 +/- 0.1, H = 4.3 +/- 0.1, K = 4.3 +/- 0.1, L = 4.5 +/- 0.2.

  12. Distribucin del continuo ultravioleta e infrarrojo en estrellas Be

    NASA Astrophysics Data System (ADS)

    Cruzado, A.; Ringuelet, A.; di Rocco, H.

    Estamos interesados en interpretar los excesos de flujo infrarrojo y/o ultravioleta observados en estrellas Be con respecto al de estrellas B normales, as como su posible correlacin. Una envoltura rodeando la estrella distingue una estrella Be de una B normal; para explicar las observaciones es necesario entonces tener en cuenta todos los posibles procesos fsicos que en ella tienen lugar. Adoptando un modelo atmosfrico especfico, hemos estudiado el proceso de recombinacin dielectrnica de metales como MgII y FeII, teniendo en cuenta la posible influencia de la opacidad del medio en el flujo emitido.

  13. Aparicin del cometa Hale - BOPP C / 1995-01

    NASA Astrophysics Data System (ADS)

    Arquiola, A.

    El Cometa Hale - Bopp fue observado a partir de la 10ma. magnitud con el telescopio de 300mm de abertura a f/6.5. En esas primeras observaciones desde luego no hubo mucho detalle. A su vez las posiciones del cometa para nuestro hemisferio no fueron favorables, solamente asom por el horizonte N.O. para nuestra latitud cuando se alejaba de la Tierra. Se registr la observacin visualmente con binoculares 10 x 50, y fotogrficamente con un Telezoom de 210mm a f/4,5 con pelcula 400 ASA color. Todas las imgenes fueron procesadas y digitalizadas a fin de obtener las diferentes escalas de brillo. El aspecto que present para nuestra posicin geogrfica fue discreta ya que tuvo su mximo brillo cuando era visible desde el hemisferio norte. Present la clsica cola de polvo curva, y con su aspecto casi estelar, destacndose entre la luminosidad del crepsculo. Instrumentos Utilizados: Telezoom de 210mm a f/4.5, Telescopio refractor de 80mm a f/15, Binocular 10 x 50

  14. 33 CFR 162.200 - Marina del Rey, Calif.; restricted area.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Marina del Rey, Calif.; restricted area. 162.200 Section 162.200 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.200 Marina del Rey, Calif.; restricted area. (a)...

  15. Aspectos generales de la Red Nacional de Estudios Clnicos del NCI

    Cancer.gov

    Resea general sobre la estructura organizativa de la Red Nacional de Estudios Clnicos del NCI, as como un resumen de los cambios que se estn llevando a cabo y una sinopsis de cmo estos cambios se basan en el xito del programa de Grupos Cooperativos.

  16. [Recommendations to improve the scientific comunication process in the Revista Médica del IMSS].

    PubMed

    Álvarez, Ivón

    2016-01-01

    In order to improve the position of the Revista Médica del Instituto Mexicano del Seguro Social among the different journals, in this editorial we enumerate a series of recommendations to ameliorate the practices of the different actors who participate in the scientific communication process of this journal. PMID:26820191

  17. 60. C.J.T., photographer December 23, 1955 KLAMATH RIVER BRIDGE, DEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    60. C.J.T., photographer December 23, 1955 KLAMATH RIVER BRIDGE, DEL NORTE COUNTY, SECTION A, HIGHWAY 1. DN-1-A #538, KLAMATH RIVER BR. FROM SO. END, 12/23/55, C.J.T. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  18. 62. R.L.T., photographer November 1, 1934 DEL NORTE COUNTY, SECTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    62. R.L.T., photographer November 1, 1934 DEL NORTE COUNTY, SECTION D, HIGHWAY 1. REDWOOD CLEARING ON EXISTING LINE, 1-DN-71-A #26, R.L.T. 11-1-34. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  19. Primer registro para Peru del genero Nielsonia Young, 1977 (Hemiptera: Cicadellidae: Cicadellinae: Cicadellini)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    En este articulo se reporta por primera vez para el Peru una especies del genero Nielsonia Young, 1977, de material procedente del Departamento de Tumbes. El genero ha sido reportada anteriormente de Ecuador, como unico registro para Sudamerica, y America Central. El unico especimen hembra encontra...

  20. Instantánea del cáncer de cuello uterino

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de cuello uterino; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  1. 63. G.F.H., photographer July 30, 1932 DEL NORTE COUNTY, SECTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    63. G.F.H., photographer July 30, 1932 DEL NORTE COUNTY, SECTION A, HIGHWAY 1. 1-DN A #124, STA. 164=00 SHOWING DRAINAGE CONDITIONS, G.F.H., 7-30-32. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  2. 61. M.C., photographer June 22, 1951 KLAMATH, DEL NORTE COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    61. M.C., photographer June 22, 1951 KLAMATH, DEL NORTE COUNTY, SECTION A, HIGHWAY 1. DN-1-A #452 NORTHERLY THROUGH KLAMATH FROM STA. 325+50, 6/22/51, M.C. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  3. Instantánea del cáncer de ovario

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de ovario; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  4. 33 CFR 162.200 - Marina del Rey, Calif.; restricted area.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Marina del Rey, Calif.; restricted area. 162.200 Section 162.200 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.200 Marina del Rey, Calif.; restricted area. (a)...

  5. 66. Photographer unknown March 1920'S DEL NORTE COUNTY, SECTION B, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    66. Photographer unknown March 1920'S DEL NORTE COUNTY, SECTION B, HIGHWAY 1. 1-DN 1-B #9, CORDUROY DETOUR INTO CRESCENT CITY, 2-2 (3?). Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  6. Instantánea del cáncer de estómago

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de estómago; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  7. 33 CFR 162.200 - Marina del Rey, Calif.; restricted area.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Marina del Rey, Calif.; restricted area. 162.200 Section 162.200 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.200 Marina del Rey, Calif.; restricted area. (a)...

  8. Instantánea del cáncer de tiroides

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de tiroides; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  9. 33 CFR 334.110 - Delaware Bay off Cape Henlopen, Del.; naval restricted area.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Del.; naval restricted area. 334.110 Section 334.110 Navigation and Navigable Waters CORPS OF....110 Delaware Bay off Cape Henlopen, Del.; naval restricted area. (a) The area. Beginning at a point on... regulations in this section shall be enforced by the Commandant, Fourth Naval District, and such agencies...

  10. 33 CFR 334.110 - Delaware Bay off Cape Henlopen, Del.; naval restricted area.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Del.; naval restricted area. 334.110 Section 334.110 Navigation and Navigable Waters CORPS OF....110 Delaware Bay off Cape Henlopen, Del.; naval restricted area. (a) The area. Beginning at a point on... regulations in this section shall be enforced by the Commandant, Fourth Naval District, and such agencies...

  11. 33 CFR 334.110 - Delaware Bay off Cape Henlopen, Del.; naval restricted area.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Del.; naval restricted area. 334.110 Section 334.110 Navigation and Navigable Waters CORPS OF....110 Delaware Bay off Cape Henlopen, Del.; naval restricted area. (a) The area. Beginning at a point on... regulations in this section shall be enforced by the Commandant, Fourth Naval District, and such agencies...

  12. 33 CFR 334.110 - Delaware Bay off Cape Henlopen, Del.; naval restricted area.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., Del.; naval restricted area. 334.110 Section 334.110 Navigation and Navigable Waters CORPS OF....110 Delaware Bay off Cape Henlopen, Del.; naval restricted area. (a) The area. Beginning at a point on... regulations in this section shall be enforced by the Commandant, Fourth Naval District, and such agencies...

  13. Instantánea del cáncer de seno (mama)

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de seno (mama); así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  14. Instantánea del cáncer de pulmón

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de pulmón; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  15. Instantánea del cáncer de páncreas

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de páncreas; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  16. Violacin del Principio de Equivalencia en Teoras con Dilatn de Cuerdas

    NASA Astrophysics Data System (ADS)

    Landau, S. J.; Sisterna, P. D.; Vucetich, H.

    Se estudian las violaciones al Principio de Equivalencia en Teoras con Dilatn de Cuerdas. En estos modelos, algunas de las constantes fundamentales dependen del espacio y del tiempo. Se muestra que los experimentos de cada libre no tienen an precisin como para poner lmites a los parmetros de la teora.

  17. Instantánea del cáncer de endometrio

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de endometrio; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  18. Instantánea del cáncer de esófago

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de esófago; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  19. Instantánea del cáncer de vejiga

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre el cáncer de vejiga; así como ejemplos de actividades del NCI y adelantos en la investigación de este tipo de cáncer.

  20. El té y la prevención del cáncer

    Cancer.gov

    Hoja informativa que resume los resultados de estudios realizados sobre el té y la prevención del cáncer. Incluye información sobre los ingredientes del té y las consideraciones de seguridad sobre el consumo de esta bebida.

  1. Efecto del Programa de Entrenamiento “Manejo del Dolor” en la Documentación de Enfermería en el Expediente Electrónico

    PubMed Central

    Monsiváis, María Guadalupe Moreno; Guzmán, Ma. Guadalupe Interial; Flores, Paz Francisco Sauceda; Arreola, Leticia Vázquez

    2012-01-01

    Resumen En el presente trabajo se muestra la importancia de entrenar al personal de enfermería para mejorar la documentación en el expediente electrónico. Se eligió el manejo del dolor por ser un área prioritaria; una alta proporción de pacientes en período post operatorio cursa con dolor, por lo tanto, la documentación debe ser útil para la toma de decisiones clínicas. Se implementó un programa de entrenamiento denominado “Manejo del Dolor” dirigido al personal de enfermería. Se utilizó la tecnología de la información como herramienta para fortalecer el conocimiento con base en la revisión sistemática de la literatura; el personal de enfermería participante seleccionó la mejor evidencia; posteriormente se trabajó en la transferencia de este conocimiento a la práctica a través del diseño de un protocolo para el manejo del dolor. Se concluye que el conocimiento del manejo del dolor es fundamental para que enfermería documente con mayor precisión sus intervenciones. PMID:24199106

  2. Assessing Glacier Hazards At Ghiacciaio Del Belvedere, Macugnaga, Italian Alps

    NASA Astrophysics Data System (ADS)

    Haeberli, W.; Chiarle, M.; Mortara, G.; Mazza, A.

    The uppermost section of the Valle Anzasca behind and above the community of Macugnaga in the Italian Alps is one of the most spectacular high-mountain land- scapes in Europe, with gigantic rock walls and numerous steep hanging glaciers. Its main glacier, Ghiacciaio del Belvedere at the foot of the huge Monte Rosa east face, is a heavily debris-covered glacier flowing on a thick sediment bed. Problems with floods, avalanches and debris flows from this ice body have been known for extended time periods. Most recently, however, the evolution of this highly dynamic environ- ment has become more dramatic. An outburst of Lago delle Locce, an ice-dammed lake at the confluenec of the tributary Ghiacciaio delle Locce with Ghiacciaio del Belvedere, caused heavy damage in 1979 and necessitated site investigation and con- struction work to be done for flood protection. The intermittent glacier growth ten- dency in the 1970es induced strong bulging of the glacier surface and, in places, caused the glacier tongue to override historical morains and to destroy newly-grown forest stands. A surge-type flow acceleration started in the lower parts of the Monte- Rosa east face during summer 2000, leading to strong crevassing and deformation of Ghiacciaio del Belvedere and extreme bulging of its orographic right margin. High water pressure and accelerated movement lasted into winter 2001/2002: the ice now started overriding the LIA moraine near Rifugio Zamboni of the CAI. In addition but rather independently, a most active detachment zone for rock falls and debris flows developed for several years now in the east face of Monte Rosa, somewhat more to the south of the accelerated glacier movement and at an altitude where relatively warm permafrost must be expected. Besides the scientific interest in these phenomena, the growing hazard potential to the local infrastructure must be considered seriously. Es- pecially potentials for the destabilization of large rock and ice masses in the east face of Monte Rosa, for interruption of the access trail to the Rifugio Zamboni by overrid- ing and/or collapse of the lateral moraine, for impacts by ice invading various instal- lations (cable car, restaurant, ski runs, trails) near the moraine hill of Belvedere, and for sudden outbursts of pressurized subglacial water or the newly formed supraglacial lake must be envisaged.

  3. Disruption of cytokeratin-8 interaction with F508del-CFTR corrects its functional defect.

    PubMed

    Colas, Julien; Faure, Grazyna; Saussereau, Emilie; Trudel, Stphanie; Rabeh, Wael M; Bitam, Sara; Guerrera, Ida Chiara; Fritsch, Janine; Sermet-Gaudelus, Isabelle; Davezac, Nolie; Brouillard, Franck; Lukacs, Gergely L; Herrmann, Harald; Ollero, Mario; Edelman, Aleksander

    2012-02-01

    We have previously reported an increased expression of cytokeratins 8/18 (K8/K18) in cells expressing the F508del mutation of cystic fibrosis transmembrane conductance regulator (CFTR). This is associated with increased colocalization of CFTR and K18 in the vicinity of the endoplasmic reticulum, although this is reversed by treating cells with curcumin, resulting in the rescue of F508del-CFTR. In the present work, we hypothesized that (i) the K8/K18 network may interact physically with CFTR, and that (ii) this interaction may modify CFTR function. CFTR was immunoprecipitated from HeLa cells transfected with either wild-type (WT) CFTR or F508del-CFTR. Precipitates were subjected to 2D-gel electrophoresis and differential spots identified by mass spectrometry. K8 and K18 were found significantly increased in F508del-CFTR precipitates. Using surface plasmon resonance, we demonstrate that K8, but not K18, binds directly and preferentially to the F508del over the WT human NBD1 (nucleotide-binding domain-1). In vivo K8 interaction with F508del-CFTR was confirmed by proximity ligation assay in HeLa cells and in primary cultures of human respiratory epithelial cells. Ablation of K8 expression by siRNA in F508del-expressing HeLa cells led to the recovery of CFTR-dependent iodide efflux. Moreover, F508del-expressing mice topically treated with K8-siRNA showed restored nasal potential difference, equivalent to that of WT mice. These results show that disruption of F508del-CFTR and K8 interaction leads to the correction of the F508del-CFTR processing defect, suggesting a novel potential therapeutic target in CF. PMID:22038833

  4. Disruption of cytokeratin-8 interaction with F508del-CFTR corrects its functional defect

    PubMed Central

    Colas, Julien; Faure, Grazyna; Saussereau, Emilie; Trudel, Stphanie; Rabeh, Wael M.; Bitam, Sara; Guerrera, Ida Chiara; Fritsch, Janine; Sermet-Gaudelus, Isabelle; Davezac, Nolie; Brouillard, Franck; Lukacs, Gergely L.; Herrmann, Harald; Ollero, Mario; Edelman, Aleksander

    2012-01-01

    We have previously reported an increased expression of cytokeratins 8/18 (K8/K18) in cells expressing the F508del mutation of cystic fibrosis transmembrane conductance regulator (CFTR). This is associated with increased colocalization of CFTR and K18 in the vicinity of the endoplasmic reticulum, although this is reversed by treating cells with curcumin, resulting in the rescue of F508del-CFTR. In the present work, we hypothesized that (i) the K8/K18 network may interact physically with CFTR, and that (ii) this interaction may modify CFTR function. CFTR was immunoprecipitated from HeLa cells transfected with either wild-type (WT) CFTR or F508del-CFTR. Precipitates were subjected to 2D-gel electrophoresis and differential spots identified by mass spectrometry. K8 and K18 were found significantly increased in F508del-CFTR precipitates. Using surface plasmon resonance, we demonstrate that K8, but not K18, binds directly and preferentially to the F508del over the WT human NBD1 (nucleotide-binding domain-1). In vivo K8 interaction with F508del-CFTR was confirmed by proximity ligation assay in HeLa cells and in primary cultures of human respiratory epithelial cells. Ablation of K8 expression by siRNA in F508del-expressing HeLa cells led to the recovery of CFTR-dependent iodide efflux. Moreover, F508del-expressing mice topically treated with K8-siRNA showed restored nasal potential difference, equivalent to that of WT mice. These results show that disruption of F508del-CFTR and K8 interaction leads to the correction of the F508del-CFTR processing defect, suggesting a novel potential therapeutic target in CF. PMID:22038833

  5. [History of Instituto Nacional de Salud Ocupacional del Peru].

    PubMed

    Cossio-Brazzan, Juan M

    2012-06-01

    In Peru, the industry's development has made economic improvements but at the same time, it has had a major impact on the health of the workers; for that reason, it was necessary to generate control mechanisms. So, in 1940 it was created the Departmento de Higiene Industrial, which in 1956 was changed to Instituto de Salud Ocupacional, but it was deactivated in 1994. However, in 2001 it reappeared into the Ministerio de Salud organizational structure with the name of Instituto de Salud Ocupacional "Alberto Hurtado Abadía". Actually, it is the Centro Nacional de Salud Ocupacional y Protección del Ambiente para la Salud (CENSOPAS), organ of the Instituto Nacional de Salud which continues working in synergy with other institutions and sectors, making research to protect the health of exposed persons (workers and community) to contamination and risks associated with economic activities. PMID:22858781

  6. Clean cities: Award winning coalition -- Paso del Norte

    SciTech Connect

    O'Connor, K.

    1999-10-25

    Designated the 41st Clean Cities coalition in November 1995, the Paso del Norte Clean Cities Coalition (PDNCCC) is the first in the country to gain international participation. Spanning the US-Mexico border; the coalition includes stakeholders from El Paso, Texas; Ciudad Juarez, Mexico; and Las Cruces, New Mexico. PDNCCC developed a comprehensive plan to jump-start its program place, alternative fuel vehicles (AFVs) on the road, and eliminate barriers inhibiting alternative fuel market growth. PDNCC raised more than $2.3 million for alternative fuel activities and clean air initiatives in less than 26 months. In 1998, the US Department of Energy (DOE) recognized that PDNCCC accomplishment with its Rainmaker Award for leveraging the most funds from outside sources. PDNCCC is proud of its efforts to drive the alternative fuels and AFV market in the El Paso/Juarez region.

  7. Energy integrated farm system: Del Valle Hog Farm

    SciTech Connect

    Not Available

    1984-01-01

    The Del Valle Hog Farm, a Texas hog farm with grain crops, is designed to conserve energy through methane generation, alcohol production, and efficient use of electrical energy. The integrated energy concepts to be demonstrated are: methane generation from swine manure to produce electricity and heat for alcohol fermentation and distillation and to produce hot water for heating the digester reactors and the hog feeding pens; and alcohol production from milo and distillation of 170 proof alcohol by use of methane and generated electricity. Specific energy technologies to be implemented are: anaerobic digester; gas compressors to store methane for peak demands; engine generator powered by methane; waste-heat exchanger on engine generator to produce hot water; continuous-process alcohol facility to produce 24 gal/day; and crop management.

  8. Spatially Controlled Fe Isotope Variations at Torres del Paine

    NASA Astrophysics Data System (ADS)

    Gajos, N.; Lundstrom, C.

    2013-12-01

    Recent advances in mass-spectrometry have identified systematic trends of non-traditional stable isotope variation in igneous rocks with differentiation index. We present new Fe isotope data for the Torres del Paine igneous complex in southern Chile. The multi-composition pluton consists of a 1 km vertical exposure of homogenous granite overlying a contemporaneous and possibly cogenetic 0.5 km mafic gabbro suite. Whereas previous isotopic investigations do little to address variations across important magmatic contacts, this study focuses on a first-of-its-kind spatially dependent non-traditional stable isotope investigation of an igneous pluton. Samples were collected at Torres del Paine in spatially significant transects, focusing on major contacts between country rock, granite and mafic units. Results collected by bracketed double spike MC-ICP-MS (2s precision of 0.03) show an increase in ?56Fe towards the high silica margins of the pluton with values as high as ?56Fe 0.36. Additionally, the data show a decrease in ?56Fe toward the mafic center of the pluton with ?56Fe values ranging from ?56Fe -0.05 to 0.18. Samples collected on the contact between the granite and mafic complex show intermediate values of ?56Fe= 0.18() 0.03. Country rock samples in contact with granite show an isotopically light signature of ?56Fe=0.04 () 0.03. Analysis of 50 samples in total show a trend of increasing ?56Fe with SiO2 content. The process responsible for Fe isotope variations remains debated but is suggested to reflect four mechanisms: (1) crustal assimilation, (2) fractional crystallization, (3) late stage fluid exsolution [1] and (4) thermal migration [3]. Preliminary results show that mechanisms #1 and #2 would produce isotopic signatures opposite of those seen at Torres del Paine and other plutonic rocks. Isotopically light Torres country rock samples reveal that assimilation of rocks would not produce the isotopically heavy granites seen at Torres. Based on experimental results [1] fractional crystallization involving magnetite-melt fractionation should produce isotopically light melts, contrary to the isotopic signature seen in Torres. On the other hand, magnetite-fluid experiments show that late-stage fluid exsolution in plutons has the potential to produce isotopic signatures similar to Torres del Paine [1]. However, the amount of fractionation is highly dependent on the amount of Fe saturated in the fluid. The fractionation trend of Fe is also consistent with thermal migration experiments [3]. The spatial partitioning of heavy Fe at the felsic margins of the pluton and light Fe at the mafic base of the pluton is also consistent with this mechanism. The co-variation with other non-traditional stable isotope systems provides a crucial test to assessing the effects of thermal migration. [1] Belinker et al. (2012) AGU Meeting Abstract. [2] Heimann A. et al. (2008) GCA 72, 4379-4396.[3] Lundstrom (2009) GCA 73, 5709-5729.

  9. Circular structures of Bajada del Diablo (Argentina): geophysical signatures

    NASA Astrophysics Data System (ADS)

    Prezzi, C. B.; Orgeira, M. J.; Martinez, O.; Acevedo, R. D.; Ponce, F.; Goldmann, G.; Magneres, I.; Rabassa, J.

    2016-02-01

    Bajada del Diablo is located in the Northern Patagonian Massif, Chubut, Argentina. The study area includes several circular structures found in Miocene olivine basalts of the Quiñelaf Eruptive Complex and in the Late Pliocene/Early Pleistocene Pampa Sastre conglomerates. An impact origin has been proposed for these circular structures. With the aim of further investigate the proposed impact origin, topographic, gravimetric, magnetic, resistivity, paleomagnetic and electromagnetic surveys in two circular structures ("8" and "G") located in Pampa Sastre conglomerates and in basalts of the Quiñelaf Eruptive Complex were carried out. The new geophysical results support the hypothesis of an impact origin. However, the confirmation of such an origin through the findings of shock metamorphism evidences and/or the recovery of meteorites remains elusive.

  10. Ozone studies in the Paso del Norte region

    NASA Astrophysics Data System (ADS)

    Becerra-Davila, Fernando

    The Paso del Norte region forms the largest contiguous bi-national conglomerate on the US-Mexico border. With a combined population of around 2 million inhabitants, the Paso del Norte region is isolated, more than 500 km away from the nearest urban area of comparable size, thus making it an ideal location for air quality studies of an isolated urban environment. The meteorological conditions leading to a high ozone episode in this region, such as the historical ozone episode of June 2006, are analyzed. It is well known that stagnation and minimal winds, high temperatures, and pressure ridges over the region are conducive to high ozone episodes. In addition, the planetary boundary height is studied to understand its impact on high ozone episodes. Several studies report that ground level ozone non-attainment regulations could be caused not only by local emissions, but also by atmospheric transport. In this work the atmospheric advection of pollutants into the region is analyzed using HYSPLIT backward trajectories. Furthermore, a novel backward trajectory clustering technique is implemented for the summer of 2006. The "ozone weekend effect" (OWE) is a phenomenon by which in some geographical regions ambient ozone concentrations tend to be higher on weekends than on weekdays, despite the lower emissions of ozone precursors during those days. The observed local OWE has never previously been studied in terms of the photolysis rates of four of the main ozone precursors. In this research a novel method that allows the calculation of actinic fluxes, photolysis frequencies and photolysis rates with a high degree of accuracy and reliability has been developed. This method utilizes a combination of the experimental data available for this region in conjunction with a radiative transfer model (TUV model). Three weekend-weekday cases during summers 2006, 2009 and 2010 are studied in this work. In this research, the photolysis impact on the local OWE is studied. The results obtained from this photolysis study demonstrate that the local ground level ozone formation is not only influenced by the strong solar radiation and changing aerosol makeup, but also by other heterogeneous factors and reactions. In addition, this research provided good evidence that the ground level ozone precursor regime in El Paso during the ozone episode of June 2006 was mostly VOC-limited. Much of this estimation was derived from measurements of local ambient VOC/NOx ratios. This finding shows that at least during June 2006, the non-linear surface ozone production increased during weekends compared to workdays in a habitually VOC-limited regime. The seasonal variations of columnar ozone as measured by a Multi-filter Rotating Shadowband instrument installed at the UTEP campus are analyzed for the first time for this region and results are presented. This investigation has addressed the problem of ground-level ozone formation in the Paso del Norte region. Urban ozone is a complex problem with many aspects that are not fully understood. In this investigation, a range of techniques has been used to address the study of local surface ozone episodes with the purpose of acquiring new insights and knowledge that will help understand and remediate the diverse atmospheric pollution events that affect this bi-national region recurrently. Innovative techniques were developed and used, ranging from the use of local ambient atmospheric pollution data to the utilization of complex modeling techniques to achieve the best possible computer results. Finally, the influence of ground level ozone concentrations in admissions to hospitals for this region due to respiratory diseases is analyzed. The comprehensive results obtained in this work will help to better understand ozone formation in the Paso del Norte Region for future policy regulation implementations.

  11. Near-IR Photometry of Nova Del 2013

    NASA Astrophysics Data System (ADS)

    Cass, C. A.; Carlon, L. R.; Corgan, T. D.; Dykhoff, A. D.; Gehrz, D. R.; Shenoy, P. D.

    2013-08-01

    Subjects: Infra-Red, Nova We report Near-IR photometry of Nova Del 2013 taken with the 0.76-m infrared telescope at the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA). RIJHKLM photometry were obtained on August 21.17 and 23.13 UT using an AsSi bolometer. Vega (alpha Lyrae) was used as the standard star. Our photometry show: August 21.17 UT: R = 5.1 +/- 0.1, I = 4.6 +/- 0.1, J = 4.6 +/- 0.1, H = 4.1 +/- 0.1, K = 3.9 +/- 0.1, L = 2.7 +/- 0.2, M = +2.2 +/- 0.2.

  12. Renewable energy load assessment for Boquillas Del Carmen Coahuila, Mexico

    SciTech Connect

    Foster, R.

    1995-08-01

    This report outlines the estimates that were made in 1992 of the potential load requirements for Boquillas del Carmen, a small Mexican village on the northern border of the state of Coahuila, Mexico near Big Bend National Park in southern Texas. The study was made to help determine the possibility that village might be electrified by solar or wind energy. Various estimates of are given of the potential load based on estimates ranging from basic use of lights, radio, television, and small household appliances to microwave ovens, refrigerators, and direct evaporative coolers. The low-energy consumption case was estimated to be at 23.0 kWh/month per residence per month, and the high-energy consumption case (with cooling) was 140.7 kWh/month per residence. On average, the typical residence is occupied by five individuals.

  13. Isotope hydrology of groundwaters in the Pampa del Tamarugal, Chile

    NASA Astrophysics Data System (ADS)

    Fritz, P.; Suzuki, O.; Silva, C.; Salati, E.

    1981-09-01

    The water resources in northern Chile are extremely scarce and development is limited by this. This paper discusses the isotope hydrology of groundwaters in the Pampa del Tamarugal an area of extreme aridity (rainfall < 1 mm/yr.) whose groundwater resources are mined for the town of Iquique, as well as for industrial and agricultural purposes. The aim of the project was to obtain information on modern recharge and to delineate, if possible recharge environments. To obtain the necessary background information a precipitation survey in the high Andes, as well as spring- and surface-water studies were carried out. The results show that a well-defined meteoric water line exists where ? 2H = (7.8 ? 18O + 10.3)% , and altitude effects depend on air-mass movements and cannot be defined without a broader regional and detailed sampling programme. However, it is still possible to assign maximum altitudes of recharge to springs in the Andes and at the eastern border of the Pampa del Tamarugal. Comparison of these data with groundwater compositions show, that these groundwaters originate from infiltrating surface water rather than directly infiltrated precipitation. A dependence of individual groundwater systems on specific quebradas (river valleys) is recognized. However, low 14C activities indicate that most of the waters pumped today are fossil and at least several hundreds if not thousands of years old. Some minor subsurface recharge does occur at the foot of the Andes, especially at Pica where high-altitude waters discharge, but even there groundwater appears to be a diminishing resource.

  14. The hydrothermal system of Nevado del Ruiz Volcano, Colombia

    SciTech Connect

    Lopez, D.A.L.

    1992-01-01

    Nevado del Ruiz is an andesitic stratovolcano located 150 km northwest of Bogota, Columbia characterized by a large hydrothermal system with two very distinctive types of water: acid sulfate waters, and bicarbonate and neutral chloride waters. The waters within each group fall in well-defined lines on compositional cross plots with an apparent lack of mixing between the two water types. The acid sulfate waters appear to be related to the north-west striking and seismically active Villamaria-Termales fault. The neutral chloride waters are clustered to the west and south-east of the volcano. The bicarbonate waters are more widespread. Several hydrothermally-altered samples from Nevado del Ruiz volcano hydrothermal system were analyzed using X-ray diffraction techniques. It was possible to distinguish two sets of mineral assemblages which correspond to the two water types. The acid sulfate waters produce cristobalite, sulfates, hematite or pyrite, sulfur and minor amounts of trydimite, kaolinite, smectite, and illite. The alteration products at the neutral chloride and bicarbonate waters include mainly carbonates and cristobalite. Simulation of the reaction between the hydrothermal fluids and the basaltic andesites of the lower volcanic units at Ruiz using the computer program CHILLER yielded mineral assemblages which are consistent with the observed alteration mineralogy. High chloride and sulfate concentrations, and helium and sulfur isotopes suggest that the acid sulfate waters have an important magmatic contribution. The composition of the neutral chloride an bicarbonate waters suggests mineral equilibria with feldspars and carbonates. Both types of waters appear to be the product of mixing of high salinity end members with different proportions of meteoric water. Linear variations in composition of the gases discharged at Ruiz suggested that the gas phase also represents mixing. Faults and contacts play a key role in the circulation of fluids at Ruiz.

  15. Volcanic hazard maps of the Nevado del Ruiz volcano, Colombia

    NASA Astrophysics Data System (ADS)

    Parra, Eduardo; Cepeda, Hector

    1990-07-01

    Although the potential hazards associated with an eruption of Nevado del Ruiz volcano were known to civil authorities before the catastrophic eruption there in November 1985, their low perception of risk and the long quiescent period since the last eruption (140 years), caused them to wait for stronger activity before developing an eruption alert system. Unfortunately, the eruption occurred suddenly after a period of relative quiet, and as a result more than 25,000 people were killed. Although it was accurate and reasonably comprehensive, the hazard map that existed before the eruption was poorly understood by the authorities and even less so by the general population, because the scientific terminology and probabilistic approach to natural hazards were unfamiliar to many of them. This confusion was shared by the communication media, which at critical times placed undue emphasis on the possibility of lava flows rather than on the more imminent threat from mudflows, in keeping with the popular but often inaccurate perception of volcanic eruptions. This work presents an updated hazard map of Nevado del Ruiz that combines information on various hazardous phenomena with their relative probability of occurrence in order to depict numerical "hazard levels" that are easily comprehensible to nonspecialists and therefore less susceptible to misinterpretation. The scale of relative risk is arbitrary, ranging from five to one, and is intended to provide an intuitive indication of danger to people, property and crops. The map is meant to facilitate emergency preparedness and management by political and civil authorities, to educate the public concerning volcanic hazards and to assist in land-use planning decisions.

  16. Instantánea de los cánceres de cerebro y del sistema nervioso central

    Cancer.gov

    Información sobre las tendencias de incidencia, mortalidad y financiamiento del NCI sobre los cánceres de cerebro y del sistema nervioso central; así como ejemplos de actividades del NCI y adelantos en la investigación de estos tipos de cáncer.

  17. DEL-1 restrains osteoclastogenesis and inhibits inflammatory bone loss in nonhuman primates.

    PubMed

    Shin, Jieun; Maekawa, Tomoki; Abe, Toshiharu; Hajishengallis, Evlambia; Hosur, Kavita; Pyaram, Kalyani; Mitroulis, Ioannis; Chavakis, Triantafyllos; Hajishengallis, George

    2015-09-30

    DEL-1 (developmental endothelial locus-1) is an endothelial cell-secreted protein that regulates LFA-1 (lymphocyte function-associated antigen-1) integrin-dependent leukocyte recruitment and inflammation in various tissues. We identified a novel regulatory mechanism of DEL-1 in osteoclast biology. Specifically, we showed that DEL-1 is expressed by human and mouse osteoclasts and regulates their differentiation and resorptive function. Mechanistically, DEL-1 inhibited the expression of NFATc1, a master regulator of osteoclastogenesis, in a Mac-1 integrin-dependent manner. In vivo mechanistic analysis has dissociated the anti-inflammatory from the anti-bone-resorptive action of DEL-1 and identified structural components thereof mediating these distinct functions. Locally administered human DEL-1 blocked inflammatory periodontal bone loss in nonhuman primates-a relevant model of human periodontitis. The ability of DEL-1 to regulate both upstream (inflammatory cell recruitment) and downstream (osteoclastogenesis) events that lead to inflammatory bone loss paves the way to a new class of endogenous therapeutics for treating periodontitis and perhaps other inflammatory disorders. PMID:26424570

  18. Observaciones del CH interestelar y el continuo en 3,3 GHz

    NASA Astrophysics Data System (ADS)

    Olano, C. A.; Combi, J. A.; Pppel, W.; Benaglia, P.; Sanz, A. J.; Bava, J. A.

    Se informa sobre el proyecto que se lleva a cabo en el IAR con el propsito de observar las lneas hiperfinas del estado fundamental del CH y el continuo en la banda de 3,3 GHz. El nuevo receptor construdo en nuestro laboratorio para tal fin se instal sobre uno de los radiotelescopios, funcionando conjuntamente con los sistemas de procesamiento actuales del IAR. Los resultados de las primeras observaciones, realizadas tanto en las lneas espectrales como en el continuo sobre fuentes conocidas, fueron satisfactorios.

  19. Spatial shell structure of the novas DQ Her and HR Del

    SciTech Connect

    Pilyogin, L.S.

    1986-03-01

    This paper considers the formation of the spatial shell structure of the novas DQ Her (1934) and HR Del (1967). It is shown that the existence of polar caps is due to the influence of the second component of the system on the angular distribution of the mass shell depth of the mainshell. The ratio of the mass shell depth in the equatorial ring and in the polar caps is 1.13 for DQ Her and 2.7 for HR Del. The ratio of the maximal densities in the equatorial ring and the polar caps is of order 3 for DQ Her and of order 70 for HR Del.

  20. Resultados del relevamiento de HI en el Cielo Austral: 3. Relevamiento de Nubes de Alta Velocidad

    NASA Astrophysics Data System (ADS)

    Morras, R.; Bajaja, E.; Arnal, E. M.; Pppel, W. G. L.

    Los resultados del relevamiento de HI del Hemisferio Austral fueron reprocesados con el fin de incrementar su sensibilidad. As, se utiliz esta nueva base de datos con el fin de obtener un nuevo relevamiento de Nubes de Alta Velocidad en el cielo austral. El ruido r.m.s. alcanzado es de 0.015-0.020 K, con una resolucin espectral de 8 km/seg. El cubrimiento espacial del relevamiento mejora en un factor 16 al realizado por Bajaja et al (1985).

  1. RASTREO DEL CANCER COLORRECTAL CONOCIMIENTO Y ACTITUD DE LA POBLACION

    PubMed Central

    CASAL, ENRIQUE R.; VELAZQUEZ, ELIZABETH N.; MEJIA, RAUL M.; CUNEO, ALDO; PEREZ-STABLE, ELISEO J.

    2014-01-01

    Resumen El rastreo de cáncer colorrectal (CCR) cuenta con fuertes evidencias en su favor. Datos preliminares indican que a pesar de ello no se lleva a cabo con la frecuencia adecuada. Se intenta aquí determinar, dentro de un Sistema de Salud que cuenta con los recursos necesarios, los elementos que facilitan o generan barreras para concretar esta práctica preventiva, cuántos individuos lo ponen en práctica y qué predice esta conducta. Se realizó una encuesta telefónica a los afiliados de una Obra Social de empleados de la Universidad de Buenos Aires, de los que 132 completaron el cuestionario (tasa de respuesta 70%). Los elementos considerados facilitadores del rastreo obtuvieron respuestas afirmativas en el 64 a 97%, mientras que los que definían barreras un 11 a 27%. En este último grupo, una categoría diferenciada la constituía el miedo a los efectos adversos: 39%, y el sentimiento de vergüenza relacionado con los procedimientos: 30%. Un 33% de los encuestados tenían hecho un método de rastreo, mayoritariamente de sangre oculta (27), sigmoideoscopía (11) y colonoscopía (20). Una mayoría afirmó que “se haría el procedimiento si el médico se lo recomendara” (95%), o “no se lo haría excepto que su médico se lo aconseje” (87%). Contestar afirmativamente que “los médicos hacen lo mejor para los pacientes” se asoció con haberse hecho un método de rastreo de CCR, OR 1.55 (IC 95%: 1.02-2.37) p: 0.04. El grupo de individuos estudiado parece bien predispuesto para el rastreo del CCR, la recomendación médica sería aquí un determinante prominente para ponerlo en práctica. PMID:19414294

  2. Paso del Norte ozone study VOC measurements, 1996

    SciTech Connect

    Seila, R.L.; Main, H.; Arriaga, J.L.; Martinez, G.V.; Ramadan, A.B.

    1999-11-01

    The results of VOC determinations of ambient air samples collected at surface air quality monitoring sites and near sources of interest on the US and Mexican side of the border during six weeks of the 1996 Paso del Norte Ozone Study are reported. Carbonyl samples were collected on DNPH impregnated cartridges at three surface sites and analyzed by HPLC to quantify 13, C-1 to C-8 species. Whole air samples were collected in electro-polished stainless steel canisters which were returned to laboratory for determination of C-2 to C-10+ hydrocarbons by cryogenic preconcentration capillary gas chromatography with flame ionization detection (gc-fid). Several sources were sampled: rush hour traffic, propane-powered bus exhaust, automobile paint shop emissions, propane fuel, petroleum refinery, and industrial manufacturing site. Spatial and temporal characteristics of VOC species concentrations and compositions are presented. Overall surface TNMOC values ranged from 0.1 to 3.4 ppmC with the highest concentrations recorded in the morning at three vehicle-dominated sites, two in Cuidad Juarez and one in downtown El Paso. Toluene in El Paso samples and propane, which is used as a cooking and transportation fuel in Cuidad Juarez, were the most abundant hydrocarbons.

  3. Paso del Norte ozone study VOC measurements, 1996

    SciTech Connect

    Seila, R.L.; Main, H.; Arriaga, J.L.; Martinez, G.V.; Ramadan, A.B.

    1999-01-01

    The results of VOC determinations of ambient air samples collected at surface air quality monitoring sites and near sources of interest on the US and Mexican side of the border during six weeks of the 1996 Paso del Norte Ozone Study are reported. Carbonyl samples were collected on DNPH impregnated cartridges at three surface sites and analyzed by HPLC to quantify 13, C-1 to C-8 species. Whole air samples were collected in electro-polished stainless steel canisters which were returned to laboratory for determination of C-2 to C-10+ hydrocarbons by cryogenic preconcentration capillary gas chromatography with flame ionization detection (gc-fid). Several sources were sampled: rush hour traffic, propane-powered bus exhaust, automobile paint shop emissions, propane fuel, petroleum refinery, and industrial manufacturing site. Spatial and temporal characteristics of VOC species concentrations and compositions are presented. Overall surface TNMOC values ranged from 0.1 to 3.4 ppmC with the highest concentrations recorded in the morning at three vehicle-dominated sites, two in Cuidad Juarez and one in downtown El Paso. Toluene in El Paso samples and propane, which is used as a cooking and transportation fuel in Cuidad Juarez, were the most abundant hydrocarbons.

  4. Status and perspectives of the INFN Laboratori Nazionali del Sud

    NASA Astrophysics Data System (ADS)

    Lattuada, M.

    2011-12-01

    The nuclear research carried out at the Laboratori Nazionali del Sud is mainly based on two ion accelerators, a 15 MV Tandem and a K800 superconductive cyclotron. The large variety of stable and radioactive beams produced and their wide energy range, together with the availability of high performance detection systems, allow for experimental research in the fields of the nuclear structure, reaction mechanisms and nuclear astrophysics. A continuous upgrading of the beam production and detection facilities is performed to mantain the role of the LNS in the panorama of the European nuclear laboratories. In addition the research activity in astroparticle physics led to the construction of two underwater laboratories in front of the Sicilian coast. They allow to check and validate submarine technologies in view of the realization of the European project KM3NeT, consisting in the installation of an observatory for high energy cosmic neutrinos in deep sea. All these experimental resources also represent formidable tools for applied researches that cover multidisciplinary fields concerning proton therapy, cultural heritage, seismology, environmental sciences and many others.

  5. Seismicity following the 1985 eruption of Nevado del Ruiz, Colombia

    USGS Publications Warehouse

    Zollweg, J.E.

    1990-01-01

    Over 200 of the best-recorded earthquakes occurring near Nevado del Ruiz, Colombia, between December 1985 and May 1986 are examined for spatial patterns, information on local stress orientations, and evidence for seismogenic structures that may be related to magma-transport processes. These earthquakes occurred following the devastating eruption of November 13, 1985. Earthquake activity that is probably related to the volcanic system occurs over a large area, of the order of 50 km2. A pattern of earthquakes beneath the southern half of Ruiz may be the expression of a conduit system, but evidence is inconclusive due to low spatial resolution of most hypocenters. Epicenter and first-motion plots suggest most earthquakes occur on small faults of varying orientation. Most seismicity during this time period was unrelated to mapped faults, including some with Holocene offsets, except for the Villamaria Fault near Ruiz's northeast sector. Composite focal mechanisms show a variation of nearly 90?? in the strike of T-axes and indicate Ruiz is in a zone of local extension. ?? 1990.

  6. 90. Paso Del Norte Hotel, 115117 South El Paso St.,east ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    90. Paso Del Norte Hotel, 115-117 South El Paso St.,east facade, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  7. Medicin de densidades medias de meteoritos: test del mtodo de inmersin

    NASA Astrophysics Data System (ADS)

    Steren, G.

    Se evalu una tcnica simple para medir las densidades medias de meteoritos, basada en el Mtodo de Arqumedes y que utiliza cuentas de vidrio de 40? en lugar de un fludo esto presenta la ventaja de no ser intrusivo ni qumicamente reactivo (D.Britt and G.Consolmagno, 1996, B.A.A.S.28,1106). El estudio, realizado en junio de este ao por participantes de la VI Escuela de Verano del Observatorio del Vaticano, emple 37 muestras de la coleccin del Observatorio del Vaticano, de las cuales 26 eran Condritas, 1 Pallasita y 1 Howardita; algunas de ellas ya habian sido estudiadas por otras tcnicas aunque tambin se incluyeron muestras no estudiadas anteriormente.

  8. The DEL1 mutator gene in Saccharomyces cerevisiae does not act in trans.

    PubMed

    Picologlou, S; Liebman, S W

    1985-01-01

    DEL1 strains of the yeast Saccharomyces cerevisiae exhibit a high rate of deletions of the three linked genes, CYC1, OSM1, and RAD7. Classical genetic methods showed that DEL1 segregated as a single Mendelian gene closely linked to CYC1. In addition, genetic evidence suggested that DEL1 was both cis- and trans-dominant (Liebman et al. 1979). Molecular analysis of deletions isolated from a haploid DEL1 strain established that deletion formation was mediated by recombination between yeast transposable elements, Ty's (Liebman et al. 1981). We now report the molecular characterization of deletions isolated from diploids in the trans configuration. This analysis reveals that these deletions probably arose in a two-step process involving mitotic recombination followed by Ty-mediated deletion formation in cis. PMID:2836088

  9. Qu es la medicina de precisin en el tratamiento del cncer

    Cancer.gov

    Informacin sobre el papel que tiene la medicina de precisin en el tratamiento del cncer. Incluye cmo se identifican los cambios genticos en el cncer de una persona y pueden usarse para seleccionar tratamientos con ms probabilidad de ayudarle.

  10. Mini-mastoidectoma para anastomosis hipogloso-facial con seccin parcial del nervio hipogloso

    PubMed Central

    Campero, lvaro; Ajler, Pablo; Socolovsky, Mariano; Martins, Carolina; Rhoton, Albert

    2012-01-01

    Introduccin: La anastomosis hipogloso-facial es la tcnica de eleccin para la reparacin de la parlisis facial cuando no se dispone de un cabo proximal sano del nervio facial. La tcnica de anastomosis mediante fresado mastoideo y seccin parcial del hipogloso minimiza la atrofia lingual sin sacrificar resultados a nivel facial. Mtodo: La porcin mastoidea del nervio facial transcurre por la pared anterior de la AM, a un promedio de 18+/-3 mm de profundidad respecto de la pared lateral. Se debe reconocer la cresta supramastoidea, desde la cual se marca una lnea vertical paralela al eje mayor de la AM, 1 cm por detrs de la pared posterior del CAE El fresado se comienza desde la lnea medio mastoidea hasta la pared posterior del CAE. Una vez encontrado el nervio facial en el tercio medio del canal mastoideo, el mismo es seguido hacia proximal y distal. Resultados: El abordaje descripto permite acceder al nervio facial intratemporal en su porcin mastoidea, y efectuar un fresado seo sin poner en riesgo al nervio o a estructuras vasculares cercanas. Se trata de un procedimiento tcnicamente ms sencillo que los abordajes amplios habitualmente utilizados al hueso temporal; no obstante su uso debe ser restringido mayormente a la anastomosis hipogloso-facial. Conclusin: Esta es una tcnica relativamente sencilla, que puede ser reproducida por cirujanos sin mayor experiencia en el tema, luego de su paso por el laboratorio de anatoma. PMID:23596555

  11. Unravelling druggable signalling networks that control F508del-CFTR proteostasis.

    PubMed

    Hegde, Ramanath Narayana; Parashuraman, Seetharaman; Iorio, Francesco; Ciciriello, Fabiana; Capuani, Fabrizio; Carissimo, Annamaria; Carrella, Diego; Belcastro, Vincenzo; Subramanian, Advait; Bounti, Laura; Persico, Maria; Carlile, Graeme; Galietta, Luis; Thomas, David Y; Di Bernardo, Diego; Luini, Alberto

    2015-01-01

    Cystic fibrosis (CF) is caused by mutations in CF transmembrane conductance regulator (CFTR). The most frequent mutation (F508del-CFTR) results in altered proteostasis, that is, in the misfolding and intracellular degradation of the protein. The F508del-CFTR proteostasis machinery and its homeostatic regulation are well studied, while the question whether 'classical' signalling pathways and phosphorylation cascades might control proteostasis remains barely explored. Here, we have unravelled signalling cascades acting selectively on the F508del-CFTR folding-trafficking defects by analysing the mechanisms of action of F508del-CFTR proteostasis regulator drugs through an approach based on transcriptional profiling followed by deconvolution of their gene signatures. Targeting multiple components of these signalling pathways resulted in potent and specific correction of F508del-CFTR proteostasis and in synergy with pharmacochaperones. These results provide new insights into the physiology of cellular proteostasis and a rational basis for developing effective pharmacological correctors of the F508del-CFTR defect. PMID:26701908

  12. CHEK2 c.1100delC allele is rarely identified in Greek breast cancer cases.

    PubMed

    Apostolou, Paraskevi; Fostira, Florentia; Papamentzelopoulou, Myrto; Michelli, Maria; Panopoulos, Christos; Fountzilas, George; Konstantopoulou, Irene; Voutsinas, Gerassimos E; Yannoukakos, Drakoulis

    2015-04-01

    The CHEK2 gene encodes a protein kinase that plays a crucial role in maintenance of genomic integrity and the DNA repair mechanism. CHEK2 germline mutations are associated with increased risk of breast cancer and other malignancies. From a clinical perspective, the most significant mutation identified is the c.1100delC mutation, which is associated with an approximately 25% lifetime breast cancer risk. The distribution of this mutation shows wide geographical variation; it is more prevalent in the Northern European countries and less common, or even absent, in Southern Europe. In order to estimate the frequency of the CHEK2 c.1100delC mutation in Greek breast cancer patients, we genotyped 2,449 patients (2,408 females and 41 males), which was the largest series ever tested for c.1100delC. The mean age of female and male breast cancer diagnosis was 49 and 59 years, respectively. All patients had previously tested negative for the Greek BRCA1 founder and recurrent mutations. The CHEK2 c.1100delC mutation was detected in 0.16% (4 of 2,408) of females, all of whom were diagnosed with breast cancer before the age of 50 years. Only one c.1100delC carrier was reported with breast cancer family history. The present study indicates that the CHEK2 c.1100delC mutation does not contribute substantially to hereditary breast cancer in patients of Greek descent. PMID:25835597

  13. Targeting F508del-CFTR to develop rational new therapies for cystic fibrosis

    PubMed Central

    Cai, Zhi-wei; Liu, Jia; Li, Hong-yu; Sheppard, David N

    2011-01-01

    The mutation F508del is the commonest cause of the genetic disease cystic fibrosis (CF). CF disrupts the function of many organs in the body, most notably the lungs, by perturbing salt and water transport across epithelial surfaces. F508del causes harm in two principal ways. First, the mutation prevents delivery of the cystic fibrosis transmembrane conductance regulator (CFTR) to its correct cellular location, the apical (lumen-facing) membrane of epithelial cells. Second, F508del perturbs the Cl− channel function of CFTR by disrupting channel gating. Here, we discuss the development of rational new therapies for CF that target F508del-CFTR. We highlight how structural studies provide new insight into the role of F508 in the regulation of channel gating by cycles of ATP binding and hydrolysis. We emphasize the use of high-throughput screening to identify lead compounds for therapy development. These compounds include CFTR correctors that restore the expression of F508del-CFTR at the apical membrane of epithelial cells and CFTR potentiators that rescue the F508del-CFTR gating defect. Initial results from clinical trials of CFTR correctors and potentiators augur well for the development of small molecule therapies that target the root cause of CF: mutations in CFTR. PMID:21642944

  14. 1996 Paso del Norte ozone study VOC measurements

    SciTech Connect

    Seila, R.L.; Main, H.; Arriaga, J.L.; Martinez, G.; Ramadan, A.B.

    1999-07-01

    Ambient air VOC samples were collected at surface air quality monitoring sites, near sources of interest, and aloft on the US and Mexican side of the border during a six week period of the 1996 Paso del Norte Ozone Study. On nine intensive operations (IOP) days, when high ozone concentrations were forecast, five 2-hr samples were collected at five IOP sites, three on the US side and two on the Mexican side. Six special survey sites on the US side and two on the Mexican side were sampled to characterize up-wind, down-wind and other emissions. In Ciudad Juarez, rush hour traffic, propane-powered bus exhaust, automobile paint shop emissions, propane and butane fuels, and an industrial manufacturing site were sampled. Carbonyl samples were collected at three surface sites. Carbonyl and canister grab samples were also collected during aircraft and hot air balloon flights. Most of the hydrocarbon samples were collected in electro-polished stainless steel canisters which were returned to laboratories for determination of C-2 to C-10+ hydrocarbons by cryogenic preconcentration GC-FID. The carbonyl samples were collected on DNPH impregnated C-18 Sep-Pak cartridges and analyzed by HPLC to quantify 13, C-1 to C-8 species. This paper presents the spatial and temporal characteristics of VOC species concentrations and compositions to examine the differences and similarities of the various locations and time periods. Overall surface TNMOC values ranged from 0.1 to 3.4 ppmC with the highest concentrations being recorded in the morning at three vehicle-dominated sites, two in Ciudad Juarez and one in downtown El Paso. Toluene in El Paso samples and propane, which is used as a cooking and transportation fuel in Ciudad Juarez, were the most abundant hydrocarbons. The most abundant carbonyls were acetaldehyde and acetone.

  15. Bidimensional spectroscopy of HR Del shell with GMOS-IFU

    NASA Astrophysics Data System (ADS)

    Augusto, A.; Diaz, M.

    2006-06-01

    The slow and bright classical nova HR Del 1967 was observed in the optical using GEMINI-NORTH GMOS-IFU in 2002 with a spectral range from 0.4 μm to 1 μm, a resolution of ˜ 300 km/s, and spatial resolution of ˜ 0''.5. Line intensity maps were obtained for Hα +[NII], [OIII] 5007, Hβ and helium lines. The shell showed a bipolar and clumped structure resembling the shell observed in 1998 (Harman and O'Brien 2003). The [OIII] 5007 map showed seven bright knots. Models of the central source, shell and knots spectra were performed using CLOUDY 94 (Ferland et al. 1998) and RA3D (Diaz 2001). Central source spectra were best fitted with a T_{eff} = 60000 K, log(g) = 6 and log(L_*) = 36.6 Rauch atmosphere model. Shell H-density law was modeled using a power law with α parameter -1.2 and mass = 4.1 × 10^{-4} M_⊙. The knots spectra were fitted as gaussian condensations with ˜ 50000cm^{-3} peak H-density and ˜ 20000cm^{-3} average H-density. HeII 4686 emission was found in the knots. The HeII origin is atributted to projected emission from diffuse matter in front of the knots, hypothesis corroborated by about the same HeII intensity observed outside the knots. Physical parameters of the condensations were obtained from our analysis and helium abundances were recalculated. A peak density contrast N/N_S = 270 was found when compared to the medium outside the knots. Electron temperatures ˜ 9500 K, electron densities ˜ 20000cm^{-3} and helium number abundance ˜ 0.2 were derived. Our results showed a constant helium abundance within a narrow radius range which is consistent with impulsive ejection (Kato and Hachisu 1994).

  16. El uso de la neuromodulación para el tratamiento del temblor

    PubMed Central

    Bendersky, Damián; Ajler, Pablo; Yampolsky, Claudio

    2014-01-01

    Introducción: El temblor puede ser un desorden incapacitante y el tratamiento de primera línea para estos pacientes es farmacológico. Sin embargo, este tratamiento puede llevar a una reducción satisfactoria del temblor en sólo el 50% de los pacientes con temblor esencial. La talamotomía era el tratamiento de elección para el temblor refractario al tratamiento médico hasta que comenzó a utilizarse la estimulación cerebral profunda (ECP) del núcleo ventral intermedio (Vim) del tálamo. En la actualidad, raramente se realiza la talamotomía. Métodos: Este artículo es una revisión no sistemática de las indicaciones, resultados, parámetros de programación y técnica quirúrgica de la ECP del Vim para el tratamiento del temblor. Resultados: Aunque los resultados clínicos son similares usando la talamotomía o la ECP del Vim, la primera causa más efectos adversos que la última. Además, la ECP puede ser usada bilateralmente, mientras que la talamotomía tiene un alto riesgo de causar disartria cuando se realiza de ambos lados. La ECP del Vim logró una adecuada mejoría del temblor en varias series de pacientes con temblor causado por temblor esencial, enfermedad de Parkinson o esclerosis múltiple. Además del Vim, hay otros blancos que están siendo usados por varios autores, tales como la zona incerta y las radiaciones prelemniscales. Conclusión: La ECP del Vim es un tratamiento útil para el temblor incapacitante refractario al tratamiento médico. Es esencial realizar una precisa selección de pacientes, así como utilizar una técnica quirúrgica correcta. Aún se desconoce el mejor blanco estereotáctico para el temblor, aunque el Vim es el más usado. PMID:25165613

  17. Manipulating proteostasis to repair the F508del-CFTR defect in cystic fibrosis.

    PubMed

    Esposito, Speranza; Tosco, Antonella; Villella, Valeria R; Raia, Valeria; Kroemer, Guido; Maiuri, Luigi

    2016-12-01

    Cystic fibrosis (CF) is a lethal monogenic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that entails the (diagnostic) increase in sweat electrolyte concentrations, progressive lung disease with chronic inflammation and recurrent bacterial infections, pancreatic insufficiency, and male infertility. Therapies aimed at restoring the CFTR defect have emerged. Thus, a small molecule which facilitates chloride channel opening, the potentiator Ivacaftor, has been approved for the treatment of CF patients bearing a particular class of rare CFTR mutations. However, small molecules that directly target the most common misfolded CFTR mutant, F508del, and improve its intracellular trafficking in vitro, have been less effective than expected when tested in CF patients, even in combination with Ivacaftor. Thus, new strategies are required to circumvent the F508del-CFTR defect. Airway and intestinal epithelial cells from CF patients bearing the F508del-CFTR mutation exhibit an impressive derangement of cellular proteostasis, with oxidative stress, overactivation of the tissue transglutaminase (TG2), and disabled autophagy. Proteostasis regulators such as cysteamine can rescue and stabilize a functional F508del-CFTR protein through suppressing TG2 activation and restoring autophagy in vivo in F508del-CFTR homozygous mice, in vitro in CF patient-derived cell lines, ex vivo in freshly collected primary patient's nasal cells, as well as in a pilot clinical trial involving homozygous F508del-CFTR patients. Here, we discuss how the therapeutic normalization of defective proteostasis can be harnessed for the treatment of CF patients with the F508del-CFTR mutation. PMID:26976279

  18. [Radiological percutaneous gastrostomy (GRP), 17 aos de experiencia, serie de casos del hospital Universitario Virgen del Roco].

    PubMed

    Navarro Falcn, Magnolia Del Carmen; Parejo Campos, Juana; Pereira Cunill, Jos Luis; Garca Luna, Pedro Pablo

    2015-01-01

    When assessing a patient nutritional support the state of the gastrointestinal tract is the most important point to consider, whenever possible we should choose the enteral nutrition (EN) over parenteral nutrition (PN) and recognized by its various advantages. The percutaneous gastrostomy (GRP) were introduced in clinical practice as an alternative to surgical gastrostomy (GQ). The speed, simplicity, low cost, low morbidity and low mortality of these techniques has allowed its rapid development.The feeding tubes types used were the balloon-retained devices catheter, the pig tail and the ballon-retained with gastropexia (gastropexia). It is a retrospective study of all patients who were placed GPR in the Virgen del Roco University Hospital, Sevilla, between September 1996 and September 2013, which aims to study is to describe the characteristics of patients and the complications presented for different types of enteral feeding tubes used. GPR 186 were performed in 176 patients (135 males (76.70%), with an average duration of 303.6 days, the most frequent diseases were cancers of the head and neck 49.46%. The types of feeding tubes used were pigtail 118 (63.44%), balloon-retained devices 22 (11.83%), and gastropexia 46 (24.73%). The most common early complications in the pigtail group were early purulent exudate and early bleeding (2.5 and 3.4% respectively), while in the balloon catheter group the initial output of the probe was most frequent early complication (13.7%), none of these complications were observed in the group of gastropexy. The most common late complication was obstruction probe. The GPR is a safe technique with lower mortality of 1%; Low frequently of early and late complications. A better understanding of this technique can reduce the frequency of complications. PMID:25561125

  19. High mountain soils and periglacial features at the Torres del Paine, National Park Torres del Paine, Chile.

    NASA Astrophysics Data System (ADS)

    Senra, Eduardo; Schaefer, Carlos; Simas, Felipe; Gjorup, Davi

    2015-04-01

    The Torres del Paine National Park (TPNP) is located on the southern limit of the Andean Southern Ice Field, part of the Magallanes and Antartica Chilena region, in the province of Ultima Esperanza. The TPNP has a very heterogeneous climate due to orographic influence and wet air masses from the Pacific. The geology is basically Cretaceous metasedimentary rocks and Miocene granitic plutons and batholiths. We studied the main soils and geoenvironments of Mt Ferrier mountain and its surroundings, based on soils , landforms and vegetation aspects. The geoenvironmental stratification was based on the combined variation and integration of pedo-litho-geomorphological features with the vegetation. WE used detailed geological maps, a DEM and slope maps and WorlView II satellite images. Fifteen soils profiles were sampled and classified according to Soil Taxonomy (2010) at all genovironments, ranging from 50 m a.s.l to the at high plateau just below the permanent snowline, under periglacial conditions (~1004m asl). Three soil temperature and moisture monitoring sites were set, allowing for 24 consecutive months (2011 to 2013). Seven geoenvironments were identified with distinct soil and landform characteristics, all with a similar geological substrate. The landform and vegetation have a strong connection with the landscape dynamic, controlling erosional and depositional processes, resulting from glacier advances and retreats in the Late Quaternary. Wind blown materials is widespread, in the form of loess material, accumulating in the higher parts of the landscape. On the other hand, accumulation of organic matter in the water-saturated depressions is common in all altitudes. Generally the soils are acidic and dystrophic, with little exceptions. The following geoenvironments were identified: Periglacial Tundra, Loess slopes, Talus and scarpmentd, Fluvio-glacial terraces, Fluvio-lacustrine plains, Moraines and Paleodunes. The regional pedology show the occurrence of five soil orders (Soil Taxonomy, 2010): Histosols, Mollisols, Inceptsols, Entisols and Andisols.

  20. Del(20q) in patients with chronic lymphocytic leukemia: A therapy-related abnormality involving lymphoid or myeloid cells

    PubMed Central

    Yin, C. Cameron; Tang, Guilin; Lu, Gary; Feng, Xiaoli; Keating, Michael J.; Medeiros, L. Jeffrey; Abruzzo, Lynne V.

    2015-01-01

    Del(20q), a common cytogenetic abnormality in myeloid neoplasms, is rare in chronic lymphocytic leukemia. We report 64 patients with chronic lymphocytic leukemia and del(20q), as the sole abnormality in 40, a stemline abnormality in 21, and a secondary abnormality in 3 cases. FISH analysis revealed an additional high-risk abnormality, del(11q) or del(17p), in 27/64 (42%) cases. In most cases, the leukemic cells showed atypical cytologic features, unmutated IGHV genes and ZAP70 positivity. The del(20q) was detected only after chemotherapy in all 27 cases with initial karyotypes available. With a median follow-up of 90 months, 30 patients (47%) died, most as a direct consequence of chronic lymphocytic leukemia. Eight patients developed a therapy-related myeloid neoplasm, seven with a complex karyotype. Combined morphologic and FISH analysis for del(20q) performed in 12 cases without morphologic evidence of a myeloid neoplasm localized the del(20q) to the chronic lymphocytic leukemia cells in 5 (42%) cases, and to myeloid/erythroid cells in 7 (58)% cases. The del(20q) was detected in myeloid cells in all 4 cases of myelodysplastic syndrome. In aggregate, these data indicate that chronic lymphocytic leukemia with del(20q) acquired after therapy is heterogeneous. In cases with morphologic evidence of dysplasia, the del(20q) likely resides in the myeloid lineage. However, in cases without morphologic evidence of dysplasia, the del(20q) may represent clonal evolution and disease progression. Combining morphologic analysis with FISH for del(20q) or performing FISH on immunomagnetically-selected subpopulations to localize the cell population with this abnormality may help guide patient management. PMID:25953391

  1. Using Del-1 to Tip the Angiogenic Balance in Endothelial Cells in Modular Constructs

    PubMed Central

    Ciucurel, Ema C.; Vlahos, Alexander E.

    2014-01-01

    Modular tissue engineering is a method of building vascularized tissue-engineered constructs. Submillimeter-sized collagen pieces (modules) coated with a layer of endothelial cells (EC; vascular component), and with embedded functional cells, are self-assembled into a larger, three-dimensional tissue. In this study, we examined the use of developmental endothelial locus-1 (Del-1), an extracellular matrix protein with proangiogenic properties, as a means of tipping the angiogenic balance in human umbilical vein endothelial cells incorporated in modular tissue-engineered constructs. The motivation was to enhance the vascularization of these constructs upon transplantation in vivo, in this case, without the use of exogenous mesenchymal stromal cells. EC were transduced using a lentiviral construct to overexpress Del-1. The Del-1 EC formed more sprouts in a fibrin gel sprouting assay in vitro compared with eGFP (control) transduced EC, as expected. Del-1 EC had a distinct profile of gene expression (upregulation of matrix metalloproteinase-9 [MMP-9], urokinase-type plasminogen activator [uPA/PLAU], vascular endothelial growth factor [VEGF-A], and intercellular adhesion molecule-1 [ICAM-1]; downregulation of angiopoietin-2 [Ang2]), also supporting the notion of “tipping the angiogenic balance”. On the other hand, contrary to our expectations, when Del-1 EC-coated modules were implanted subcutaneously in a severe combined immunodeficient/beige animal model, the proangiogenic effect of Del-1 was less remarkable. There was only a small increase in the number of blood vessels formed in Del-1 implants compared with the eGFP implants, and only few blood vessels formed at the implant site in both cases. This was presumed due to limited EC survival after transplantation. We speculate that if we could improve EC survival in our study (for example, by adding other prosurvival factors or supporting cells), we would see a greater Del-1-induced angiogenic benefit in vivo as a consequence of increased Del-1 secretion by a higher number of surviving cells. PMID:24138448

  2. La implantacion del enfoque constructivista en el aula de ciencia: Estudio de caso multiple

    NASA Astrophysics Data System (ADS)

    Arroyo Betancourt, Luz I.

    Esta investigacion estudia la implantacion del enfoque constructivista en tres aulas de ciencia del contexto puertorriqueno. Se auscultaron las practicas educativas que utilizan maestras consideradas constructivistas y la correspondencia de sus practicas educativas con los elementos esenciales de la didactica que proponen los teoricos de los planteamientos constructivistas. Se ausculto, ademas, a que vision del enfoque constructivista responden las expresiones de las maestras acerca de su practica educativa y como compara con su quehacer, a la luz de los elementos esenciales de las visiones constructivistas piagetiana, social y radical. Se utilizo el diseno de estudio descriptivo de caso multiple. El estudio se baso en entrevistas a profundidad, revision de documentos y observacion no participativa a la sala de clases. El contexto fueron tres escuelas publicas de la Region Educativa de San Juan, una elemental, una intermedia y una superior. Los resultados confirmaron que la transicion hacia el enfoque constructivista es un proceso que toma tiempo, dedicacion y la participacion en adiestramientos y readiestramientos acerca del nuevo enfoque. Las maestras coinciden en la mayoria de las practicas educativas que utilizan para implantar el enfoque constructivista de ensenanza y difieren en algunas debido, probablemente, a que han tenido que adaptarlas a los correspondientes niveles de ensenanza: elemental, intermedio y superior. Dos de las maestras planifican por conceptos generadores, mientras que una de ellas planifica siguiendo la guia que recibe del Departamento de Educacion. Difieren ademas, en el enfasis que confieren al inquirir cientifico. Con relacion a la correspondencia entre la vision manifestada por las maestras a la luz de las visiones piagetiana, social y radical, aparentemente, las preguntas del protocolo de entrevistas no lograron evocar la informacion con suficiente profundidad, por lo que la investigadora tuvo que inferir las visiones de las participantes basado en lo que estas manifestaron en su practica didactica. Dos maestras coinciden en una vision constructivista social de la construccion del conocimiento, del aprendizaje y de los metodos didacticos. La otra manifesto una vision constructivista piagetiana en el aprendizaje, los metodos didacticos y en la construccion del conocimiento. Se espera que este trabajo, ademas de promover los estudios de caso sobre el enfoque constructivista de ensenanza en el contexto puertorriqueno, sirva para que los maestros, que estan transformando su enfoque educativo de uno tradicional a uno constructivista, tengan una vision mas clara de la implantacion de este enfoque. Se espera ademas que sirva para que el Departamento de Educacion y sus programas de adiestramiento y readiestramiento en servicio, asi como las universidades y sus programas de preparacion de maestros, tomen en cuenta los resultados y recomendaciones de este estudio al revisar sus programas.

  3. The RGD finger of Del-1 is a unique structural feature critical for integrin binding

    SciTech Connect

    Schürpf, Thomas; Chen, Qiang; Liu, Jin-huan; Wang, Rui; Springer, Timothy A.; Wang, Jia-huai

    2012-11-13

    Developmental endothelial cell locus-1 (Del-1) glycoprotein is secreted by endothelial cells and a subset of macrophages. Del-1 plays a regulatory role in vascular remodeling and functions in innate immunity through interaction with integrin {alpha}{sub V}{beta}{sub 3}. Del-1 contains 3 epidermal growth factor (EGF)-like repeats and 2 discoidin-like domains. An Arg-Gly-Asp (RGD) motif in the second EGF domain (EGF2) mediates adhesion by endothelial cells and phagocytes. We report the crystal structure of its 3 EGF domains. The RGD motif of EGF2 forms a type II' {beta} turn at the tip of a long protruding loop, dubbed the RGD finger. Whereas EGF2 and EGF3 constitute a rigid rod via an interdomain calcium ion binding site, the long linker between EGF1 and EGF2 lends considerable flexibility to EGF1. Two unique O-linked glycans and 1 N-linked glycan locate to the opposite side of EGF2 from the RGD motif. These structural features favor integrin binding of the RGD finger. Mutagenesis data confirm the importance of having the RGD motif at the tip of the RGD finger. A database search for EGF domain sequences shows that this RGD finger is likely an evolutionary insertion and unique to the EGF domain of Del-1 and its homologue milk fat globule-EGF 8. The RGD finger of Del-1 is a unique structural feature critical for integrin binding.

  4. Biotic association and palaeoenvironmental reconstruction of the "Loma del Pterodaustro" fossil site (Early Cretaceous, Argentina)

    USGS Publications Warehouse

    Chiappe, L.; Rivarola, D.; Cione, A.; Fregenal-Martinez, M.; Sozzi, H.; Buatois, L.; Gallego, O.; Laza, J.; Romero, E.; Lopez-Arbarello, A.; Buscalioni, A.; Marsicano, C.; Adamonis, S.; Ortega, F.; McGehee, S.; Di, Iorio O.

    1998-01-01

    A sedimentological analysis of the basal section of the Early Cretaceous, lacustrine Lagarcito Formation at "Loma del Pterodaustro" (San Luis, Argentina) and a summary of its biological components are presented. Three sedimentological facies can be recognized in the basal sequence of the Lagarcito Formation. Fossil remains are particularly abundant in laminated claystones of a facies interpreted as deposits formed in offshore areas of the lake. The preservation of delicate structures allows recognition of these deposits as a Konservat Lagersta??tte. Up to now, rocks at "Loma del Pterodaustro" have yielded plants, conchostracans, semionotid and pleuropholid fishes, pterodactyloid pterosaurs, and a variety of invertebrate traces. The chronology of the Lagarcito Formation is discussed and it is concluded that this unit is of Albian age. The palaeoenvironment of deposition of the basal sequence of the Lagarcito Formation at "Loma del Pterodaustro" is interpreted as a perennial, shallow lake developed within an alluvial plain, under semiarid climatic conditions.

  5. Integrated global background monitoring network. Preliminary results from Torres del Paine and Olympic National Parks

    SciTech Connect

    Wiersma, G.B.; Kohler, A.; Boelcke, C.; Baker, G.; Harmon, M.; Weber, C.; Gonzales, J.

    1985-10-01

    During 1984, a pilot project was initiated for monitoring pollution at Torres del Paine National Park in southern Chile and Olympic National Park in the United States. These are two of three initial sites that are to be established as part of an integrated global backgound monitoring network. Eventually, the plan is to establish a world-wide system of such sites. We collected and analyzed samples of the soil, water, air, and two species of plants (moss and lichen). We also collected and analyzed samples of the forest litter. We compared the samples of soil and vegetation against reference samples. We also compared samples of soil, vegetation, and of organic material from Torres del Paine against similar samples from Olympic and Sequoia-Kings Canyon National Parks in the United States. Although the data is preliminary, it is in agreement with out initial hypothesis that Torres del Paine and Olympic National Parks are not a polluted sites.

  6. Deep source model for Nevado del Ruiz Volcano, Colombia, constrained by interferometric synthetic aperture radar observations

    NASA Astrophysics Data System (ADS)

    Lundgren, Paul; Samsonov, Sergey V.; López Velez, Cristian Mauricio; Ordoñez, Milton

    2015-06-01

    Nevado del Ruiz is part of a large volcano complex in the northern Andes of Colombia. Interferometric synthetic aperture radar observations from the RADARSAT-2 satellite since 2011 show steady inflation of the volcano since 2012 at 3-4 cm/yr. The broad (>20 km) deformation pattern from both ascending and descending track data constrain source models for either point or spheroidal sources, both located at >14 km beneath the surface (mean elevation 4.2 km) and 10 km SW of Nevado del Ruiz, below nearby Santa Isabel Volcano. Stress change computations for both sources in the context of a compressive regional stress indicate that dikes propagating from the source should become trapped in sills, possibly leading to a more complex pathway to the surface and explaining the significant lateral separation of the source and Nevado del Ruiz Volcano.

  7. Basis for a streamflow forecasting system to Rincn del Bonete and Salto Grande (Uruguay)

    NASA Astrophysics Data System (ADS)

    Talento, Stefanie; Terra, Rafael

    2013-10-01

    This paper presents the basis for the design of streamflow prediction systems for the hydroelectric dams of Rincn del Bonete (Uruguay) and Salto Grande (Uruguay-Argentina). The prediction is made, independently, for each reservoir and each month of the year with two methodologies: data-driven statistical models and hybrid downscaling that includes atmospheric predictors. We determine a set of potential predictors and then fit linear models coupled with variable selection techniques, under the hypothesis of perfectly known predictors. The predictive skill of the schemes outperforms the climatological forecast throughout the year in both reservoirs (except August in Rincn del Bonete). This remains the case even when the forecast lead does not allow for the use of preceding flows as predictors. While in Rincn del Bonete it is not possible to distinguish a period of high predictability, in Salto Grande, there is a robust signal in March-May and October-December.

  8. CASE REPORT: Phenotypic presentation of the Ser63Del MPZ mutation

    PubMed Central

    Miller, Lindsey J.; Patzko, Agnes; Lewis, Richard A.; Shy, Michael E.

    2013-01-01

    Mutations in MPZ cause CMT1B, the second most frequent cause of CMT1. Elegant studies with Ser63del mice suggest that Ser63del MPZ is retained in the ER where it activates the unfolded protein response (UPR) that contributes to the neuropathy. Clinical information about patients with this mutation is limited. We present clinical and electrophysiological data on a large multigenerational family with CMT1B caused by Ser63del MPZ. The patients have a classical CMT1 phenotype that is much less severe than that of patients with Arg98Cys MPZ that also activates the UPR. These results suggest that clinical presentation along cannot predict which MPZ mutations will be retained in the ER and activate the UPR. PMID:22734905

  9. La relativit debole. La fisica dello spazio e del tempo senza paradossi

    NASA Astrophysics Data System (ADS)

    Selleri, Franco

    2011-06-01

    Secondo Einstein e Poincar la simultaneit di eventi che hanno luogo in punti diversi dello spazio pu essere definita solo per convenzione. Dal punto di vista matematico si dimostra, quindi, che due diverse definizioni di simultaneit corrispondono a due diversi valori del coefficiente e1 della variabile spaziale x presente nelle trasformazioni di Lorentz. A partire da premesse normalmente accettate otterremo diverse dimostrazioni della necessit di reintrodurre il concetto di simultaneit assoluta. Le conseguenze cosmologiche della nuova struttura dello spazio e del tempo andranno contro la cosmologia del Big Bang. Inoltre, dopo questi risultati, il relativismo sopravviver in una forma meno aspra ("relativit debole") perch risulter privo di quel campionario di paradossi portato dalla teoria della Relativit Speciale.

  10. Relevamiento total del hemisferio sur celeste en la frecuencia de 1420 MHz

    NASA Astrophysics Data System (ADS)

    Bava, J. A.; Colomb, F. R.; Hurrel, E.; Larrarte, J. J.; Sanz, A. J.; Testori, J. C.; Reich, P.; Reich, W.; Wielebinski, R.

    En el presente artculo se describe el relevamiento del cielo en el Hemisferio Sur Celeste en la frecuencia de 1420 MHz para declinaciones ?<= -19o realizado con la Antena II de 30 metros de dimetro del IAR. Este relevamiento posee igual sensibilidad (3xr.m.s=50 mK) que el realizado en el Hemisferio Norte con el radiotelescopio de 25 metros de Stockert de la Universidad de Bonn, operado por el Max-Planck Institute fr Radioastronomie ( Reich W., 1982, A&ASS 48, 219; Reich P. and Reich W., 1986, A&ASS 63, 205). Con los datos obtenidos por ambos radiotelescopios se posee una base de datos de todo el cielo en esta frecuencia. En esta publicacin presentamos los detalles del sistema receptor, tcnicas de observacin y reduccin de datos, calibracin y discusin de los errores en los resultados.

  11. Pseudomonas aeruginosa Reduces VX-809 Stimulated F508del-CFTR Chloride Secretion by Airway Epithelial Cells

    PubMed Central

    Stanton, Bruce A.; Coutermarsh, Bonita; Barnaby, Roxanna; Hogan, Deborah

    2015-01-01

    Background P. aeruginosa is an opportunistic pathogen that chronically infects the lungs of 85% of adult patients with Cystic Fibrosis (CF). Previously, we demonstrated that P. aeruginosa reduced wt-CFTR Cl secretion by airway epithelial cells. Recently, a new investigational drug VX-809 has been shown to increase F508del-CFTR Cl secretion in human bronchial epithelial (HBE) cells, and, in combination with VX-770, to increase FEV1 (forced expiratory volume in 1 second) by an average of 3-5% in CF patients homozygous for the F508del-CFTR mutation. We propose that P. aeruginosa infection of CF lungs reduces VX-809 + VX-770- stimulated F508del-CFTR Cl secretion, and thereby reduces the clinical efficacy of VX-809 + VX-770. Methods and Results F508del-CFBE cells and primary cultures of CF-HBE cells (F508del/F508del) were exposed to VX-809 alone or a combination of VX-809 + VX-770 for 48 hours and the effect of P. aeruginosa on F508del-CFTR Cl secretion was measured in Ussing chambers. The effect of VX-809 on F508del-CFTR abundance was measured by cell surface biotinylation and western blot analysis. PAO1, PA14, PAK and 6 clinical isolates of P. aeruginosa (3 mucoid and 3 non-mucoid) significantly reduced drug stimulated F508del-CFTR Cl secretion, and plasma membrane F508del-CFTR. Conclusion The observation that P. aeruginosa reduces VX-809 and VX-809 + VX-770 stimulated F508del CFTR Cl secretion may explain, in part, why VX-809 + VX-770 has modest efficacy in clinical trials. PMID:26018799

  12. Correlated occurrence and bypass of frame-shifting insertion-deletions (InDels) to give functional proteins.

    PubMed

    Rockah-Shmuel, Liat; Tth-Petrczy, gnes; Sela, Asaf; Wurtzel, Omri; Sorek, Rotem; Tawfik, Dan S

    2013-10-01

    Short insertions and deletions (InDels) comprise an important part of the natural mutational repertoire. InDels are, however, highly deleterious, primarily because two-thirds result in frame-shifts. Bypass through slippage over homonucleotide repeats by transcriptional and/or translational infidelity is known to occur sporadically. However, the overall frequency of bypass and its relation to sequence composition remain unclear. Intriguingly, the occurrence of InDels and the bypass of frame-shifts are mechanistically related - occurring through slippage over repeats by DNA or RNA polymerases, or by the ribosome, respectively. Here, we show that the frequency of frame-shifting InDels, and the frequency by which they are bypassed to give full-length, functional proteins, are indeed highly correlated. Using a laboratory genetic drift, we have exhaustively mapped all InDels that occurred within a single gene. We thus compared the naive InDel repertoire that results from DNA polymerase slippage to the frame-shifting InDels tolerated following selection to maintain protein function. We found that InDels repeatedly occurred, and were bypassed, within homonucleotide repeats of 3-8 bases. The longer the repeat, the higher was the frequency of InDels formation, and the more frequent was their bypass. Besides an expected 8A repeat, other types of repeats, including short ones, and G and C repeats, were bypassed. Although obtained in vitro, our results indicate a direct link between the genetic occurrence of InDels and their phenotypic rescue, thus suggesting a potential role for frame-shifting InDels as bridging evolutionary intermediates. PMID:24204297

  13. Haplotype analysis of the 185delAG BRCA1 mutation in ethnically diverse populations

    PubMed Central

    Laitman, Yael; Feng, Bing-Jian; Zamir, Itay M; Weitzel, Jeffrey N; Duncan, Paul; Port, Danielle; Thirthagiri, Eswary; Teo, Soo-Hwang; Evans, Gareth; Latif, Ayse; Newman, William G; Gershoni-Baruch, Ruth; Zidan, Jamal; Shimon-Paluch, Shani; Goldgar, David; Friedman, Eitan

    2013-01-01

    The 185delAG* BRCA1 mutation is encountered primarily in Jewish Ashkenazi and Iraqi individuals, and sporadically in non-Jews. Previous studies estimated that this is a founder mutation in Jewish mutation carriers that arose before the dispersion of Jews in the Diaspora ?2500 years ago. The aim of this study was to assess the haplotype in ethnically diverse 185delAG* BRCA1 mutation carriers, and to estimate the age at which the mutation arose. Ethnically diverse Jewish and non-Jewish 185delAG*BRCA1 mutation carriers and their relatives were genotyped using 15 microsatellite markers and three SNPs spanning 12.5?MB, encompassing the BRCA1 gene locus. Estimation of mutation age was based on a subset of 11 markers spanning a region of ?5?MB, using a previously developed algorithm applying the maximum likelihood method. Overall, 188 participants (154 carriers and 34 noncarriers) from 115 families were included: Ashkenazi, Iraq, Kuchin-Indians, Syria, Turkey, Iran, Tunisia, Bulgaria, non-Jewish English, non-Jewish Malaysian, and Hispanics. Haplotype analysis indicated that the 185delAG mutation arose 7501500 years ago. In Ashkenazim, it is a founder mutation that arose 61 generations ago, and with a small group of founder mutations was introduced into the Hispanic population (conversos) ?650 years ago, and into the IraqiJewish community ?450 years ago. The 185delAG mutation in the non-Jewish populations in Malaysia and the UK arose at least twice independently. We conclude that the 185delAG* BRCA1 mutation resides on a common haplotype among Ashkenazi Jews, and arose about 61 generations ago and arose independently at least twice in non-Jews. PMID:22763381

  14. Development and Utilization of InDel Markers to Identify Peanut (Arachis hypogaea) Disease Resistance

    PubMed Central

    Liu, Lifeng; Dang, Phat M.; Chen, Charles Y.

    2015-01-01

    Peanut diseases, such as leaf spot and spotted wilt caused by Tomato Spotted Wilt Virus, can significantly reduce yield and quality. Application of marker assisted plant breeding requires the development and validation of different types of DNA molecular markers. Nearly 10,000 SSR-based molecular markers have been identified by various research groups around the world, but less than 14.5% showed polymorphism in peanut and only 6.4% have been mapped. Low levels of polymorphism limit the application of marker assisted selection (MAS) in peanut breeding programs. Insertion/deletion (InDel) markers have been reported to be more polymorphic than SSRs in some crops. The goals of this study were to identify novel InDel markers and to evaluate the potential use in peanut breeding. Forty-eight InDel markers were developed from conserved sequences of functional genes and tested in a diverse panel of 118 accessions covering six botanical types of cultivated peanut, of which 104 were from the U.S. mini-core. Results showed that 16 InDel markers were polymorphic with polymorphic information content (PIC) among InDels ranged from 0.017 to 0.660. With respect to botanical types, PICs varied from 0.176 for fastigiata var., 0.181 for hypogaea var., 0.306 for vulgaris var., 0.534 for aequatoriana var., 0.556 for peruviana var., to 0.660 for hirsuta var., implying that aequatoriana var., peruviana var., and hirsuta var. have higher genetic diversity than the other types and provide a basis for gene functional studies. Single marker analysis was conducted to associate specific marker to disease resistant traits. Five InDels from functional genes were identified to be significantly correlated to tomato spotted wilt virus (TSWV) infection and leaf spot, and these novel markers will be utilized to identify disease resistant genotype in breeding populations. PMID:26617627

  15. An unexpected effect of TNF-α on F508del-CFTR maturation and function

    PubMed Central

    Bitam, Sara; Urbach, Valérie; Sermet-Gaudelus, Isabelle; Hinzpeter, Alexandre; Edelman, Aleksander

    2015-01-01

    Cystic fibrosis (CF) is a multifactorial disease caused by mutations in the cystic fibrosis transmembrane conductance regulator gene ( CFTR), which encodes a cAMP-dependent Cl - channel. The most frequent mutation, F508del, leads to the synthesis of a prematurely degraded, otherwise partially functional protein. CFTR is expressed in many epithelia, with major consequences in the airways of patients with CF, characterized by both fluid transport abnormalities and persistent inflammatory responses. The relationship between the acute phase of inflammation and the expression of wild type (WT) CFTR or F508del-CFTR is poorly understood. The aim of the present study was to investigate this effect. The results show that 10 min exposure to TNF-alpha (0.5-50ng/ml) of F508del-CFTR-transfected HeLa cells and human bronchial cells expressing F508del-CFTR in primary culture (HBE) leads to the maturation of F508del-CFTR and induces CFTR chloride currents. The enhanced CFTR expression and function upon TNFα is sustained, in HBE cells, for at least 24 h. The underlying mechanism of action involves a protein kinase C (PKC) signaling pathway, and occurs through insertion of vesicles containing F508del-CFTR to the plasma membrane, with TNFα behaving as a corrector molecule. In conclusion, a novel and unexpected action of TNFα has been discovered and points to the importance of systematic studies on the roles of inflammatory mediators in the maturation of abnormally folded proteins in general and in the context of CF in particular. PMID:26594334

  16. PREVENCIN DEL VIH/SIDA EN LOS CIRCUITOS DE LEVANTE HSH: UNA ASIGNATURA PENDIENTE.

    PubMed

    Barreda, Victoria; Carballo-Dieguez, Alex; Marone, Rubn; Baln, Ivn; Pando, Mara ngeles; Avila, Mara Mercedes

    2010-12-01

    A partir de un relevamiento de tipo etnogrfico, se describen lugares de encuentro de HSH en la Ciudad de Buenos Aires y sus prcticas sexuales. El reconocimiento de tales espacios, as como las caractersticas que asumen en ellos los encuentros sexuales entre los HSH, plantean obstculos especficos en la adopcin de comportamientos preventivos y, asimismo, generan nuevos desafos para las actividades de prevencin. Se plantean las dificultades y debates conceptuales que la misma categora presenta, y sus consecuencias en el abordaje preventivo y terico-metodolgico para las ciencias sociales. Adems, se proponen nuevos interrogantes acerca de los alcances y las limitaciones del modelo preventivo del VIH/Sida para HSH. PMID:21874154

  17. Parental origin of de novo chromosome 9 deletions in del(9p) syndrome

    SciTech Connect

    Micale, M.A.; Haren, J.M.; Conroy, J.M.

    1995-05-22

    Parental origin of de novo deletions in the short arm of chromosome 9 in patients with a clinical diagnosis of del(9p) syndrome was assessed in 13 patients using polymerase chain reaction (PCR) analysis of highly polymorphic dinucleotide repeat microsatellite markers located in the putative deleted region. The deletion was found to be of paternal origin in 9 cases and of maternal origin in the remaining 4 cases, suggesting that the molecular event resulting in the deletion occurs in both male and female gametogenesis and that genomic imprinting does not appear to play a role in the pathogenesis of del(9p) syndrome. 22 refs., 1 fig.

  18. Aplicacin del Teorema de Nekhorochev para tiempos de estabilidad en Mecnica Celeste

    NASA Astrophysics Data System (ADS)

    Miloni, O.; Nez, J.; Brunini, A.

    En Mecnica Celeste, uno de los problemas centrales consiste en la determinacin de los tiempos de estabilidad. El teorema de Nekhorochev proporciona un mtodo para dicho estudio, para un sistema determinado por un hamiltoniano descripto en las variables accin-ngulo. El trabajo consiste en la acotacin tanto del potencial perturbador y de la matriz hessiana del hamiltoniano integrable para determinar luego el tiempo de estabilidad de dicho sistema, donde por estabilidad se entiende la separacin en norma infinito en el espacio de las acciones.

  19. A review on Balanites aegyptiaca Del (desert date): phytochemical constituents, traditional uses, and pharmacological activity

    PubMed Central

    Chothani, Daya L.; Vaghasiya, H. U.

    2011-01-01

    Balanites aegyptiaca Del. (Zygophyllaceae), known as ‘desert date,’ is spiny shrub or tree up to l0 m tall, widely distributed in dry land areas of Africa and South Asia. It is traditionally used in treatment of various ailments i.e. jaundice, intestinal worm infection, wounds, malaria, syphilis, epilepsy, dysentery, constipation, diarrhea, hemorrhoid, stomach aches, asthma, and fever. It contains protein, lipid, carbohydrate, alkaloid, saponin, flavonoid, and organic acid. Present review summarizes the traditional claims, phytochemistry, and pharmacology of B. aegyptiaca Del reported in scientific literature. PMID:22096319

  20. Historical survey of the national astronomical observatory in Llano del Hato

    NASA Astrophysics Data System (ADS)

    Martinez Picar, Antonio; Salas Ramirez, Henry

    2007-12-01

    The Centro de Investigaciones de Astronomia (CIDA), the Venezuelan Institute in charge for managing human- and technical resources of the National Astronomical Observatory in Llano del Hato, is one of the younger institutes in its field. However, its foundation is surrounded by a series of curious and interesting eyents that go back to the end of the 18th century. Its equatoriallocation, excellent astronomical equipment and the high-qualified personnel offers a geographical and technical advantage. The paper is a historical oveniew of events, facts and demonstrations of the human activity related to the conception, building and starting the Llano del Hato National Astronomical Observatory.

  1. 75 FR 44720 - Safety Zone; Live-Fire Gun Exercise, M/V Del Monte, James River, VA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Live-Fire Gun Exercise, M/V Del Monte... specified waters of the James River to protect mariners from the hazards associated with live fire and... conduct a live fire and explosive training event onboard the M/V Del Monte in the vicinity of the...

  2. 78 FR 6173 - Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen Kelley, Andrew Wilklund, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF TRANSPORTATION Surface Transportation Board Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen... procedural schedule. SUMMARY: In response to a petition filed on August 1, 2012, by Diana Del Grosso,...

  3. DELIVERING TIMELY AIR QUALITY, TRAFFIC, AND WEATHER INFORMATION TO YOUR COMMUNITY/THE PASO DEL NORTE ENVIRONMENTAL MONITORING PROJECT

    EPA Science Inventory

    EPA has developed a technology transfer handbook for the EMPACT Paso del Norte Project. The EMPACT Paso del Norte Environmental Monitoring Project is a mobile vehicle emissions project that involves the international community of El Paso, TX; Sundland Park, NM; and Juarez, Mexico...

  4. Resultado negativo de prueba del VPH es mejor predictor de riesgo bajo de cáncer de cuello uterino

    Cancer.gov

    De acuerdo con un estudio en el que participaron más de un millón de mujeres, los investigadores del NCI han determinado que el resultado negativo de una prueba para la detección del VPH proporciona mayor certeza o seguridad frente a riesgos futuros de cá

  5. Cambios histricos en el aporte terrgeno de la cuenca del Ro de la Plata sobre la plataforma interna Uruguaya

    NASA Astrophysics Data System (ADS)

    Marrero, Anala; Tudur, Adriana; Prez, Laura; Cua, Caroline; Muniz, Pablo

    2014-12-01

    El Ro de la Plata (RdlP) presenta significativas variaciones naturales (hidrodinmicas y oceanogrficas) asociadas a diferentes condiciones climticas. El propsito de este trabajo es inferir los cambios de aportes continentales de sedimentos y su relacin con las variaciones hidrolgicas del Ro de la Plata, a travs del anlisis de proxies sedimentolgicos y geoqumicos en testigos de sedimentos de la plataforma interna uruguaya que registran los ltimos 100 aos, aproximadamente. A partir de la datacin por 210Pb de dos testigos de sedimentos (GeoB 13813-4 y BAR1) se reconstruy la geocronologa del ambiente, y se relacion con datos de las forzantes climticas Pacific Decadal Oscillation, El Nio/La Nia Southern Oscillation, Atlantic Multidecadal Oscillation, y las anomalas hidrolgicas de los ros Paran y Uruguay. Los valores ms positivos y estables del Southern Oscillation Index, los cuales corresponden a fases La Nia, se observan en el periodo correspondiente entre 1910-1970, respecto al resto de la serie, donde se aprecia una mayor variabilidad y una tendencia hacia valores ms negativos (eventos El Nio). Se hicieron dendrogramas (clustering) jerrquicos para ambos testigos. Para el testigo GeoB 13813-4, se utiliz la relacin Ca/Ti y la granulometra, mientras que para BAR1 se recurri a variables granulomtricas y la tasa de sedimentacin. El mayor aporte continental hacia la regin de la plataforma adyacente al Ro de la Plata registrado a partir del ao 1970, podra ser el factor principal de los agrupamientos observados en los clusters para ambos testigos. Las agrupaciones mostraron una diferenciacin en la dcada de 1970, lo que estara asociado al aumento de los caudales de los ros Paran y Uruguay, durante las ltimas tres dcadas del siglo XX. Por otra parte se observa que la granulometra del testigo BAR1 present un mayor tamao de grano y ms variabilidad que en el caso del testigo GeoB 13813-4. Tambin se determin una mayor acumulacin de sedimentos a travs del tiempo en el cinturn de barro del Ro de la Plata (plataforma continental adyacente), comparado con aquel registrado en la Barra del Indio (lmite entre zona intermedia y externa del estuario). Estas diferencias podran estar relacionadas con la influencia del Ro de la Plata, el cual genera un ambiente altamente dinmico sobre la Barra del Indio y un ambiente ms estable sobre el cinturn de barro en la plataforma continental.

  6. Dropouts, Stopouts, Optouts at Del Mar College: Spring 1991. Report of a Joint Research Project.

    ERIC Educational Resources Information Center

    Luckie, Jo Ann I.; Bonham, L. Adrianne

    A research project investigated 2,313 persons who attended Del Mar College (Texas) during fall 1990 but did not enroll for spring 1991. Four sets of data were collected: (1) limited demographic information on the total student body; (2) demographic information drawn from school records to describe all nonreturnees; (3) demographic information

  7. Community Arts Programs: Cohesion and Difference Case Studies. Henry Street Settlement and El Museo del Barrio

    ERIC Educational Resources Information Center

    Kiebert-Gruen, Cathleen

    2009-01-01

    A comparative case study of two cultural institutions, Henry Street Settlement and El Museo del Barrio, founded almost eighty years apart, were involved in social justice causes and community arts. Although both of these institutions participated in the political activism of their time, they also demonstrated an important adaptability. They were

  8. Publicacin de Propuesta Presupuestaria del NCI para el Ao Fiscal 2017

    Cancer.gov

    Ayer, tuve el honor de presentar al Presidente Barack Obama el Plan Anual del NCI y Propuesta Presupuestaria para el Ao Fiscal 2017. Creo que la Propuesta Presupuestaria, como se conoce ahora, es especialmente importante este ao para la investigacin oncolgica por dos razones.

  9. Tendencias del cncer: Influjo en la atencin y en prioridades de investigacin

    Cancer.gov

    Muchas de las tendencias que observamos en el cncer estn cambiando cmo vemos el cncer y cmo respondemos a l, desde impulsar la investigacin hasta identificar las causas fundamentales de los cnceres que estn aumentando en incidencia para dar forma a la investigacin sobre el tratamiento y la prevencin del cncer.

  10. Se lanza Red Nacional de Estudios Clínicos del NCI

    Cancer.gov

    El Instituto Nacional del Cáncer (NCI) puso en marcha una nueva red de investigación de estudios clínicos con el objetivo de mejorar el tratamiento de más de 1,6 millones de estadounidenses que reciben un diagnóstico de cáncer cada año.

  11. Calcio y la prevención del cáncer

    Cancer.gov

    Hoja informativa que resume los resultados de estudios sobre el calcio y la prevención del cáncer. Incluye información sobre las recomendaciones dietéticas para calcio y la cantidad de calcio en los alimentos y en complementos de calcio.

  12. A Failed Utopia in Marcela Del Ro's "Proceso a Faubritten"

    ERIC Educational Resources Information Center

    Manickam, Samuel

    2014-01-01

    In Marcela del Ro's science fiction novel "Proceso a Faubritten," utopia comes in the form of eternal life for all of humanity, thanks to Dr. Alexander Faubritten's "Bomba L." This polyphonic work includes diaries by Faubritten and his Mexican lover, Mara Corona. In my analysis of these two diaries, I will show how

  13. 33 CFR 334.110 - Delaware Bay off Cape Henlopen, Del.; naval restricted area.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Delaware Bay off Cape Henlopen....110 Delaware Bay off Cape Henlopen, Del.; naval restricted area. (a) The area. Beginning at a point on the south shore of Delaware Bay at longitude 75°06′12″; thence to latitude 38°47′25″, longitude...

  14. Control del dolor: Apoyo para las personas con cáncer

    Cancer.gov

    Contiene información sobre las medicinas contra el dolor para pacientes con cáncer, los planes para controlarlo, cómo hablar con su equipo de atención médica sobre el dolor que usted siente y qué hacer para controlar los efectos físicos y emocionales del

  15. A tectonically controlled basin-fill within the Valle del Cauca, West-Central Colombia

    SciTech Connect

    Rine, J.M.; Keith, J.F. Jr.; Alfonso, C.A.; Ballesteros, I.; Laverde, F.; Sacks, P.E.; Secor, D.T. Jr. ); Perez, V.E.; Bernal, I.; Cordoba, F.; Numpaque, L.E. )

    1993-02-01

    Tertiary strata of the Valle del Cauca reflect a forearc/foreland basin tectonic history spanning a period from pre-uplift of the Cordillera Central to initiation of uplift of the Cordillera Occidental. Stratigraphy of the Valle del Cauca begins with Jurassic-Cretaceous rocks of exotic and/or volcanic provenance and of oceanic origin. Unconformably overlying these are Eocene to Oligocene basal quartz-rich sandstones, shallow marine algal limestones, and fine-grained fluvial/deltaic mudstones and sandstones with coalbeds. These Eocene to Oligocene deposits represent a period of low tectonic activity. During late Oligocene to early Miocene, increased tectonic activity produced conglomeratic sediments which were transported from east to west, apparently derived from uplift of the Cordillera Central, and deposited within a fluvial to deltaic setting. East-west shortening of the Valle del Cauca basin folded the Eocene to early Miocene units, and additional uplift of the Cordillera Central during the later Miocene resulted in syn-tectonic deposition of alluvial fans. After additional fold and thrust deformation of the total Eocene-Miocene basin-fill, tectonic activity abated and Pliocene-Quaternary alluvial and lacustrine strata were deposited. Within the framework of this depositional and tectonic history of the Valle del Cauca, hydrocarbon exploration strategies can be formulated and evaluated.

  16. Exploración del Sistema Solar -- Una mirada hacia el futuro - Duration: 30 seconds.

    NASA Video Gallery

    ¿Piensas que ya sabemos todo acerca de nuestro sistema solar? La realidad es que apenas hemos comenzado con lo que hay para conocer. Únete a la NASA, en el envío de misiones a los confines del sist...

  17. Urban Impact of Dissolved Metals in the Paso del Norte Segment of the Rio Grande

    ERIC Educational Resources Information Center

    Freiwan, Sumayeh Ahmad

    2013-01-01

    The Paso del Norte segment of the Rio Grande experiences two seasons per year; the (wet) irrigation season and the (dry) non-irrigation season. The goal of this study was to improve the understanding of occurrence and contribution of dissolved metals in this region during the non-irrigation season. The objectives of this study were to (1) evaluate

  18. Centro de Contacto del NCIServicio de Informacin sobre el Cncer

    Cancer.gov

    El Instituto Nacional del Cncer (NCI) ofrece informacin actualizada, precisa, confiable y fcil de entender sobre diferentes de temas de cncer. Los especialistas en informacin le pueden ayudar en el telfono 1-800-422-6237 (1-800-4-CANCER).

  19. Use of del Nido Cardioplegia for Adult Cardiac Surgery at the Cleveland Clinic: Perfusion Implications

    PubMed Central

    Kim, Kuna; Ball, Clifford; Grady, Patrick; Mick, Stephanie

    2014-01-01

    Abstract: Cardiac arrest by cardioplegia provides a reproducible and safe method to induce and maintain electromechanical cardiac quiescence. Techniques of intraoperative myocardial protection are constantly evolving. For the past three decades, modified Buckberg cardioplegia solution has been used for adult cardiac surgery at the Cleveland Clinic. This formulation serves as the crystalloid component, which is delivered 4:1 with oxygenated patients blood to crystalloid. Meanwhile, our use of the del Nido cardioplegia solution in adult patients, heretofore primarily used in pediatric cardiac surgical centers, has been increasing over the past several years. Single-dose, cold blood del Nido cardioplegia can be delivered antegrade if the duration of the operation will be limited and if there is no significant coronary artery disease or aortic insufficiency that would limit the distribution of cardioplegia. The addition of del Nido cardioplegia to our cardioplegia armamentarium allows us to customize our myocardial protection strategies for different surgical needs. This article aims to provide information on technical aspects of del Nido cardioplegia in adult cardiac surgery and its use at the Cleveland Clinic in the adult surgical population. PMID:26357803

  20. DISYUNTIVAS EN LAS CONCEPCIONES SOBRE AUTONOMA Y BENEFICENCIA QUE AFECTAN LA TERAPUTICA DEL INTENTO SUICIDA.

    PubMed

    Mondragn, Liliana; Monroy, Zuraya; Ito, Ma Emily; Medina-Mora, Dra Ma Elena

    2010-06-01

    El objetivo del trabajo es conocer las disyuntivas entre los principios de beneficencia y autonoma, que se presentan en la relacin mdico-paciente, durante la teraputica del intento de suicidio.La investigacin se realiz en dos hospitales psiquitricos de la Ciudad de Mxico. La muestra incluy a tres sujetos con intento de suicidio, mayores de 18 aos, que eran atendidos en consulta externa a causa de una lesin autoinfligida en el ltimo ao, y a tres psiquiatras que trataban a estos pacientes. La informacin se obtuvo previo consentimiento informado en entrevistas individuales. Se llev a cabo un anlisis de discurso argumentado para encontrar los significados que los participantes otorgaron a los principios bioticos y las posibles disyuntivas entre stos.Las discordancias entre la beneficencia y la autonoma estuvieron relacionadas con el beneficio del tratamiento, el respeto por los valores y las creencias de los pacientes, entre otros. Este trabajo presenta consideraciones ticas relevantes en el escenario clnico, al ofrecer al psiquiatra un anlisis biotico que le permita actuar de acuerdo con la beneficencia y respetando la autonoma del paciente frente a casos de intento de suicidio y, de esta forma procurar una mejor atencin para ellos. PMID:20830214

  1. Informe del NCI y los CDC sobre el tabaco sin humo

    Cancer.gov

    El primer informe sobre el consumo mundial del tabaco sin humo y sus consecuencias en la salud pública reveló que más de 300 millones de personas en al menos 70 países usan estos productos dañinos.

  2. Shed Some Light on the Subject: Teaching Ramon del Valle-Inclan's "Luces de bohemia"

    ERIC Educational Resources Information Center

    Parker, Jason Thomas

    2011-01-01

    This essay seeks to provide parallel and interchangeable approaches to teaching Ramon del Valle-Inclan's challenging play "Luces de bohemia". A greater understanding of the cultural and mental frameworks of the early twentieth-century Spanish spectator will permit students to penetrate the dense intertextuality that characterizes Valle's

  3. New record of Paralomis spinosissima Birstein & Vinogradov (Decapoda: Anomura: Lithodidae) from Mar del Plata, Argentina.

    PubMed

    Olgun, Nicole; Ocampo, Emiliano H; Farias, Nahuel

    2015-01-01

    The lithodid crab Paralomis spinosissima is previously known only in Sub-Antarctic waters from South Georgia Island to the Drake Passage. Here we recorded a juvenile male obtained off shores of Mar del Plata (~37S), Argentina. This new occurrence extends the distribution range of the species over 1300 km northwards in the Atlantic Ocean. PMID:26249071

  4. Urban Impact of Dissolved Metals in the Paso del Norte Segment of the Rio Grande

    ERIC Educational Resources Information Center

    Freiwan, Sumayeh Ahmad

    2013-01-01

    The Paso del Norte segment of the Rio Grande experiences two seasons per year; the (wet) irrigation season and the (dry) non-irrigation season. The goal of this study was to improve the understanding of occurrence and contribution of dissolved metals in this region during the non-irrigation season. The objectives of this study were to (1) evaluate…

  5. Dolores Del Rio, the First "Latin Invasion", and Hollywood's Transition to Sound

    ERIC Educational Resources Information Center

    Beltran, Mary

    2005-01-01

    The career and promotion of Mexican actress Dolores Del Rio as a case study of how Hollywood's shift to sound film affected Latino and Latina actors is examined. It reveals the way in which the starring opportunities for Latinos dwindled when accent and language increasingly marked them as nonwhite in the 1930s in the talkie films.

  6. Tratamiento del cáncer sin daño al corazón

    Cancer.gov

    Investigadores de los campos de oncología y de cardiología están trabajando para encontrar formas de impedir, manejar y posiblemente aun revertir los efectos secundarios cardiovasculares de ciertas terapias del cáncer.

  7. A Failed Utopia in Marcela Del Río's "Proceso a Faubritten"

    ERIC Educational Resources Information Center

    Manickam, Samuel

    2014-01-01

    In Marcela del Río's science fiction novel "Proceso a Faubritten," utopia comes in the form of eternal life for all of humanity, thanks to Dr. Alexander Faubritten's "Bomba L." This polyphonic work includes diaries by Faubritten and his Mexican lover, María Corona. In my analysis of these two diaries, I will show how…

  8. Functional Rescue of F508del-CFTR Using Small Molecule Correctors

    PubMed Central

    Molinski, Steven; Eckford, Paul D. W.; Pasyk, Stan; Ahmadi, Saumel; Chin, Stephanie; Bear, Christine E.

    2012-01-01

    High-throughput screens for small molecules that are effective in “correcting” the functional expression of F508del-CFTR have yielded several promising hits. Two such compounds are currently in clinical trial. Despite this success, it is clear that further advances will be required in order to restore 50% or greater of wild-type CFTR function to the airways of patients harboring the F508del-CFTR protein. Progress will be enhanced by our better understanding of the molecular and cellular defects caused by the F508del mutation, present in 90% of CF patients. The goal of this chapter is to review the current understanding of defects caused by F508del in the CFTR protein and in CFTR-mediated interactions important for its biosynthesis, trafficking, channel function, and stability at the cell surface. Finally, we will discuss the gaps in our knowledge regarding the mechanism of action of existing correctors, the unmet need to discover compounds which restore proper CFTR structure and function in CF affected tissues and new strategies for therapy development. PMID:23055971

  9. Plan Multinacional de Educacion del Adulto (Multinational Plan for Adult Education).

    ERIC Educational Resources Information Center

    Eduplan Informa, 1971

    1971-01-01

    This document contains part of the final report from the first meeting of the Inter-American Council on Education, Science, and Culture, held in Vina del Mar, Chile, in 1970. The report presents general policy and guidelines which should be followed in the establishment of adult elementary education programs. The general discussion covers literacy

  10. REPRODUCTIVE RHYTHMICITY OF THE ATHERINID FISH, 'COLPICHTHYS REGIS', FROM ESTERO DEL SOLDADO, SONORA, MEXICO

    EPA Science Inventory

    The reproductive rhythmicity of the false grunion, Colpichthys regis, was observed in the Estero del Soldado, Mexico during October 1982 through January 1983. Spawning runs occurred at approximately 2-week intervals during daytime high tides. These high tides coincided with new a...

  11. 78 FR 74008 - Amendment of Class E Airspace; Del Rio, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ... Del Rio, TX, area, creating additional controlled airspace at Laughlin AFB (78 FR 52716) Docket No... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... FR 9565, 3 CFR, 1959-1963 Comp., p. 389. ] Sec. 71.1 0 2. The incorporation by reference in 14 CFR...

  12. 33 CFR 162.35 - Channel of Christina River, Del.; navigation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Channel of Christina River, Del.; navigation. 162.35 Section 162.35 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.35 Channel...

  13. 33 CFR 162.35 - Channel of Christina River, Del.; navigation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Channel of Christina River, Del.; navigation. 162.35 Section 162.35 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.35 Channel...

  14. 33 CFR 162.35 - Channel of Christina River, Del.; navigation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Channel of Christina River, Del.; navigation. 162.35 Section 162.35 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.35 Channel...

  15. 33 CFR 162.35 - Channel of Christina River, Del.; navigation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Channel of Christina River, Del.; navigation. 162.35 Section 162.35 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.35 Channel...

  16. 33 CFR 162.35 - Channel of Christina River, Del.; navigation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Channel of Christina River, Del.; navigation. 162.35 Section 162.35 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS 162.35 Channel...

  17. ATMOSPHERIC VOLATILE ORGANIC COMPOUND MEASUREMENTS DURING THE 1996 PASO DEL NORTE OZONE STUDY

    EPA Science Inventory

    Ambient air VOC samples were collected at surface air quality monitoring sites, near sources of interest, and aloft on the US (El Paso) and Mexican (Ciudad Juarez) side of the border during a six-week period of the 1996 Paso del Norte Ozone Study. Samples were collected at five...

  18. Ouabain Mimics Low Temperature Rescue of F508del-CFTR in Cystic Fibrosis Epithelial Cells.

    PubMed

    Zhang, Donglei; Ciciriello, Fabiana; Anjos, Suzana M; Carissimo, Annamaria; Liao, Jie; Carlile, Graeme W; Balghi, Haouaria; Robert, Renaud; Luini, Alberto; Hanrahan, John W; Thomas, David Y

    2012-01-01

    Most cases of cystic fibrosis (CF) are caused by the deletion of a single phenylalanine residue at position 508 of the cystic fibrosis transmembrane conductance regulator (CFTR). The mutant F508del-CFTR is retained in the endoplasmic reticulum and degraded, but can be induced by low temperature incubation (29C) to traffic to the plasma membrane where it functions as a chloride channel. Here we show that, cardiac glycosides, at nanomolar concentrations, can partially correct the trafficking of F508del-CFTR in human CF bronchial epithelial cells (CFBE41o-) and in an F508del-CFTR mouse model. Comparison of the transcriptional profiles obtained with polarized CFBE41o-cells after treatment with ouabain and by low temperature has revealed a striking similarity between the two corrector treatments that is not shared with other correctors. In summary, our study shows a novel function of ouabain and its analogs in the regulation of F508del-CFTR trafficking and suggests that compounds that mimic this low temperature correction of trafficking will provide new avenues for the development of therapeutics for CF. PMID:23060796

  19. Ouabain Mimics Low Temperature Rescue of F508del-CFTR in Cystic Fibrosis Epithelial Cells

    PubMed Central

    Zhang, Donglei; Ciciriello, Fabiana; Anjos, Suzana M.; Carissimo, Annamaria; Liao, Jie; Carlile, Graeme W.; Balghi, Haouaria; Robert, Renaud; Luini, Alberto; Hanrahan, John W.; Thomas, David Y.

    2012-01-01

    Most cases of cystic fibrosis (CF) are caused by the deletion of a single phenylalanine residue at position 508 of the cystic fibrosis transmembrane conductance regulator (CFTR). The mutant F508del-CFTR is retained in the endoplasmic reticulum and degraded, but can be induced by low temperature incubation (29C) to traffic to the plasma membrane where it functions as a chloride channel. Here we show that, cardiac glycosides, at nanomolar concentrations, can partially correct the trafficking of F508del-CFTR in human CF bronchial epithelial cells (CFBE41o-) and in an F508del-CFTR mouse model. Comparison of the transcriptional profiles obtained with polarized CFBE41o-cells after treatment with ouabain and by low temperature has revealed a striking similarity between the two corrector treatments that is not shared with other correctors. In summary, our study shows a novel function of ouabain and its analogs in the regulation of F508del-CFTR trafficking and suggests that compounds that mimic this low temperature correction of trafficking will provide new avenues for the development of therapeutics for CF. PMID:23060796

  20. Determinacion de periodos fundamentales del suelo mediante vibraciones ambientales en el municipio de Humacao, Puerto Rico

    NASA Astrophysics Data System (ADS)

    Cintron Aponte, Rommel

    La tecnica de Nakamura ha sido utilizada a nivel mundial para determinar periodos fundamentales del suelo. La tecnica consiste en calcular y graficar cocientes espectrales H/V de vibraciones ambientales registradas sobre el suelo. Mediciones de vibraciones ambientales fueron tomadas en 151 lugares dentro del municipio de Humacao, localizado al este de Puerto Rico. Los datos se procesaron utilizando espectros de Fourier y espectros de potencia. La tecnica fue validada al compararla con los resultados de cocientes espectrales H/V de registros de sismos debiles y tambien con una modelacion numerica realizada con datos de un ensayo "downhole". Las graficas de los cocientes espectrales H/V fueron divididas en casos y grupos, los cuales dependen de la facilidad para identificar el periodo fundamental pico y amplitudes en frecuencias menores de 1 Hz, respectivamente. Los resultados obtenidos con ambos espectros fueron comparados y se concluye que los mismos se complementan para proveer resultados mas confiables. Se crearon mapas de periodos fundamentales, factores de amplitud, isoperiodos y clasificacion sismica de sitio. Los mapas de isoperiodos fueron realizados en las zonas mas pobladas sobre depositos de suelo. El mapa de periodos fundamentales del suelo mostro buena correlacion con la geologia local. El mapa de clasificacion sismica derivado de periodos de sitio fue comparado con el mapa de clasificacion sismica derivado de barrenos geotecnicos. El mapa de clasificacion obtenido de periodos puede sobreestimar un poco algunas clasificaciones del suelo. Sin embargo, este mapa puede proveer un estimado aproximado de la velocidad de onda de corte promedio del suelo hasta una profundidad de 100 pies (30 metros).

  1. Synonymous codon usage affects the expression of wild type and F508del CFTR.

    PubMed

    Shah, Kalpit; Cheng, Yi; Hahn, Brian; Bridges, Robert; Bradbury, Neil A; Mueller, David M

    2015-03-27

    The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel composed of 1480 amino acids. The major mutation responsible for cystic fibrosis results in loss of amino acid residue, F508 (F508del). Loss of F508 in CFTR alters the folding pathway resulting in endoplasmic-reticulum-associated degradation. This study investigates the role of synonymous codon in the expression of CFTR and CFTR F508del in human HEK293 cells. DNA encoding the open reading frame (ORF) for CFTR containing synonymous codon replacements was expressed using a heterologous vector integrated into the genome. The results indicate that the codon usage greatly affects the expression of CFTR. While the promoter strength driving expression of the ORFs was largely unchanged and the mRNA half-lives were unchanged, the steady-state levels of the mRNA varied by as much as 30-fold. Experiments support that this apparent inconsistency is attributed to nonsense mediated decay independent of exon junction complex. The ratio of CFTR/mRNA indicates that mRNA containing native codons was more efficient in expressing mature CFTR as compared to mRNA containing synonymous high-expression codons. However, when F508del CFTR was expressed after codon optimization, a greater percentage of the protein escaped endoplasmic-reticulum-associated degradation resulting in considerable levels of mature F508del CFTR on the plasma membrane, which showed channel activity. These results indicate that codon usage has an effect on mRNA levels and protein expression, for CFTR, and likely on chaperone-assisted folding pathway, for F508del CFTR. PMID:25676312

  2. Toric K3-fibred Calabi-Yau manifolds with del Pezzo divisors for string compactifications

    NASA Astrophysics Data System (ADS)

    Cicoli, Michele; Kreuzer, Maximilian; Mayrhofer, Christoph

    2012-02-01

    We analyse several explicit toric examples of compact K3-fibred Calabi-Yau three-folds. These manifolds can be used for the study of string dualities and are crucial ingredients for the construction of LARGE Volume type IIB vacua with promising applications to cosmology and particle phenomenology. In order to build a phenomenologically viable model, on top of the two moduli corresponding to the base and the K3 fibre, we demand also the existence of two additional rigid divisors: the first supporting the non-perturbative effects needed to achieve moduli stabilisation, and the second allowing the presence of chiral matter on wrapped D-branes. We clarify the topology of these rigid divisors by discussing the interplay between a diagonal structure of the Calabi-Yau volume and D-terms. Del Pezzo divisors appearing in the volume form in a completely diagonal way are natural candidates for supporting non-perturbative effects and for quiver constructions, while `non-diagonal' del Pezzo and rigid but not del Pezzo divisors are particularly interesting for model building in the geometric regime. Searching through the existing list of four dimensional reflexive lattice polytopes, we find 158 examples admitting a Calabi-Yau hypersurface with a K3 fibration and four Khler moduli where at least one of the toric divisors is a `diagonal' del Pezzo. We work out explicitly the topological details of a few examples showing how, in the case of simplicial polytopes, all the del Pezzo divisors are `diagonal', while `non-diagonal' ones appear only in the case of non-simplicial polytopes. A companion paper will use these results in the study of moduli stabilisation for globally consistent explicit Calabi-Yau compactifications with the local presence of chirality.

  3. Percepcion de los profesores universitarios acerca del concepto cultura cientifica y de sus implicaciones en el nuevo bachillerato del Recinto de Rio Piedras de la Universidad de Puerto Rico

    NASA Astrophysics Data System (ADS)

    Ramos Pastrana, Nilsa

    El Senado Academico del Recinto de Rio Piedras de la Universidad de Puerto Rico aprobo en el ano academico 2005-2006 la Certificacion 46, que contiene los lineamientos de un nuevo bachillerato. Este nuevo bachillerato introdujo cambios significativos en el curriculo tradicional. Entre ellos se encuentra la reduccion del componente de educacion general y el de Ciencias Biologicas en particular. La reduccion de creditos en el componente de Ciencias Biologicas ha obligado a reevaluar el concepto de cultura cientifica que desarrollan esos cursos. El proposito del estudio consistio en auscultar las percepciones de los profesores de las Facultades de Administracion de Empresas, Humanidades, Ciencias Sociales, Ciencias Naturales, Educacion y Estudios Generales del Recinto de Rio Piedras de la Universidad de Puerto Rico en torno al concepto de cultura cientifica, los contenidos disciplinares del curso de Ciencias Biologicas y la reduccion de creditos en el nuevo bachillerato. Las preguntas que guiaron la investigacion fueron: ¿cuales son las percepciones que tienen los profesores de las Facultades de Administracion de Empresas, Ciencias Sociales, Estudios Generales, Ciencias Naturales, Humanidades y Educacion, en torno al concepto de cultura cientifica y los contenidos disciplinares del curso de Ciencias Biologicas? ¿cuales son las percepciones que tienen los profesores de Ciencias Biologicas en torno al concepto cultura cientifica y los contenidos disciplinares del curso de Ciencias Biologicas? ¿existen diferencias significativas por facultad, genero, experiencia, rango y nombramiento en las percepciones que tienen los profesores del Recinto de Rio Piedras de la Universidad de Puerto Rico sobre los elementos que caracterizan la cultura cientifica y los contenidos biologicos que deben tener los egresados del Recinto? ¿que implicaciones curriculares tienen estos testimonios en el desarrollo del concepto de cultura cientifica en el nuevo bachillerato? Para realizar la investigacion se utilizo una metodologia cuantitativa y la herramienta para lograrlo fue una encuesta. La encuesta se analizo estadisticamente mediante analisis de frecuencias y por cientos, prueba ANOVA, prueba t, pruebas Post Hoc de Tukey y de Levene. Tambien se realizo nuevamente una prueba de confiabilidad mediante el alfa de Cronbach. Los resultados reflejaron que los profesores de las Facultades de Administracion de Empresas, Ciencias Sociales, Ciencias Naturales, Educacion, Humanidades, Estudios Generales y el Departamento de Ciencias Biologicas tienen una percepcion muy positiva del concepto de cultura cientifica, los contenidos curriculares de Ciencias Biologicas y del nuevo bachillerato.

  4. Sanger Sequencing for BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del Mutation Screen on Pap Smear Cytology Samples.

    PubMed

    Lee, Sin Hang; Zhou, Shaoxia; Zhou, Tianjun; Hong, Guofan

    2016-01-01

    Three sets of polymerase chain reaction (PCR) primers were designed for heminested PCR amplification of the target DNA fragments in the human genome which include the site of BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del respectively, to prepare the templates for direct Sanger sequencing screen of these three founder mutations. With a robust PCR mixture, crude proteinase K digestate of the fixed cervicovaginal cells in the liquid-based Papanicolaou (Pap) cytology specimens can be used as the sample for target DNA amplification without pre-PCR DNA extraction, purification and quantitation. The post-PCR products can be used directly as the sequencing templates without further purification or quantitation. By simplifying the frontend procedures for template preparation, the cost for screening these three founder mutations can be reduced to about US $200 per test when performed in conjunction with human papillomavirus (HPV) assays now routinely ordered for cervical cancer prevention. With this projected price structure, selective patients in a high-risk population can be tested and each provided with a set of DNA sequencing electropherograms to document the absence or presence of these founder mutations in her genome to help assess inherited susceptibility to breast and ovarian cancer in this era of precision molecular personalized medicine. PMID:26867194

  5. Sanger Sequencing for BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del Mutation Screen on Pap Smear Cytology Samples

    PubMed Central

    Lee, Sin Hang; Zhou, Shaoxia; Zhou, Tianjun; Hong, Guofan

    2016-01-01

    Three sets of polymerase chain reaction (PCR) primers were designed for heminested PCR amplification of the target DNA fragments in the human genome which include the site of BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del respectively, to prepare the templates for direct Sanger sequencing screen of these three founder mutations. With a robust PCR mixture, crude proteinase K digestate of the fixed cervicovaginal cells in the liquid-based Papanicolaou (Pap) cytology specimens can be used as the sample for target DNA amplification without pre-PCR DNA extraction, purification and quantitation. The post-PCR products can be used directly as the sequencing templates without further purification or quantitation. By simplifying the frontend procedures for template preparation, the cost for screening these three founder mutations can be reduced to about US $200 per test when performed in conjunction with human papillomavirus (HPV) assays now routinely ordered for cervical cancer prevention. With this projected price structure, selective patients in a high-risk population can be tested and each provided with a set of DNA sequencing electropherograms to document the absence or presence of these founder mutations in her genome to help assess inherited susceptibility to breast and ovarian cancer in this era of precision molecular personalized medicine. PMID:26867194

  6. Informe trata el descenso del cáncer y los tumores cerebrales

    Cancer.gov

    Los índices de mortalidad en los Estados Unidos de todos los cánceres en hombres y mujeres siguieron bajando entre 2003 y 2007, que es el período más reciente del que se disponen datos, según el último Informe Anual a la Nación sobre el Estado del Cáncer. El informe encuentra también que el índice general combinado de nuevos diagnósticos de cáncer en hombres y mujeres decreció un promedio un poco menos de 1% por año en el mismo período.

  7. How the early voltage clamp studies of Jos del Castillo inform "modern" neuroscience.

    PubMed

    Zottoli, Steven J

    2012-10-01

    The description of ionic currents that flow across the membrane of the squid giant axon during an action potential sparked an interest in determining whether there were similar currents in vertebrates. The preparation of choice was the node of Ranvier in single myelinated fibers in frog. Jos del Castillo spent 3 years on the United States mainland from 1956 to 1959. During that time, he collaborated with Jerome Y. Lettvin and John W. Moore. I discuss how these individuals met one another and some of their scientific discoveries using the voltage clamp to study squid giant axons and frog nodes. Much of this work was conducted at the Marine Biological Laboratory in Woods Hole, MA, and I attempt to convey a sense of the unique scientific "melting pot" that existed at the Marine Biological Laboratory and the broader effect that del Castillo had on "modern" neuroscience. PMID:22653696

  8. Correction of F508del CFTR in airway epithelium using nanoparticles delivering triplex-forming PNAs

    PubMed Central

    McNeer, Nicole Ali; Anandalingam, Kavitha; Fields, Rachel J.; Caputo, Christina; Kopic, Sascha; Gupta, Anisha; Quijano, Elias; Polikoff, Lee; Kong, Yong; Bahal, Raman; Geibel, John P; Glazer, Peter M.; Saltzman, W. Mark; Egan, Marie E.

    2015-01-01

    Cystic fibrosis (CF) is a lethal genetic disorder most commonly caused by the F508del mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. It is not readily amenable to gene therapy because of its systemic nature and challenges including in vivo gene delivery and transient gene expression. Here, we use triplex-forming PNA molecules and donor DNA in biodegradable polymer nanoparticles to correct F508del. We confirm modification with sequencing and a functional chloride efflux assay. In vitro correction of chloride efflux occurs in up to 25% of human cells. Deep sequencing reveals negligible off-target effects in partially homologous sites. Intranasal application of nanoparticles in CF mice produces changes in nasal epithelium potential differences consistent with corrected CFTR, with gene correction also detected in lung tissue. This work represents facile genome engineering in vivo with oligonucleotides using a nanoparticle system to achieve clinically relevant levels of gene editing without off-target effects. PMID:25914116

  9. 78 FR 18783 - Establishment of the R[iacute]o Grande del Norte National Monument

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... projectile points, as well as large ceramic vessels. The area is home to a rich array of archaeological... code 3295-F3 ] TD28MR13.010 [FR Doc. 2013-07406 Filed 3-27-13; 8:45 am] Billing code 4310-10-C #0; ... harshness is known as the R o Grande del Norte, and its extraordinary array of scientific and...

  10. Pío del Río Hortega and the discovery of the oligodendrocytes

    PubMed Central

    Pérez-Cerdá, Fernando; Sánchez-Gómez, María Victoria; Matute, Carlos

    2015-01-01

    Pío del Río Hortega (1882–1945) discovered microglia and oligodendrocytes (OLGs), and after Ramón y Cajal, was the most prominent figure of the Spanish school of neurology. He began his scientific career with Nicolás Achúcarro from whom he learned the use of metallic impregnation techniques suitable to study non-neuronal cells. Later on, he joined Cajal’s laboratory. and Subsequently, he created his own group, where he continued to develop other innovative modifications of silver staining methods that revolutionized the study of glial cells a century ago. He was also interested in neuropathology and became a leading authority on Central Nervous System (CNS) tumors. In parallel to this clinical activity, del Río Hortega rendered the first systematic description of a major polymorphism present in a subtype of macroglial cells that he named as oligodendroglia and later OLGs. He established their ectodermal origin and suggested that they built the myelin sheath of CNS axons, just as Schwann cells did in the periphery. Notably, he also suggested the trophic role of OLGs for neuronal functionality, an idea that has been substantiated in the last few years. Del Río Hortega became internationally recognized and established an important neurohistological school with outstanding pupils from Spain and abroad, which nearly disappeared after his exile due to the Spanish civil war. Yet, the difficulty of metal impregnation methods and their variability in results, delayed for some decades the confirmation of his great insights into oligodendrocyte biology until the development of electron microscopy and immunohistochemistry. This review aims at summarizing the pioneer and essential contributions of del Río Hortega to the current knowledge of oligodendrocyte structure and function, and to provide a hint of the scientific personality of this extraordinary and insufficiently recognized man. PMID:26217196

  11. Antimicrobial activity of flavonoids in medicinal plants from Taf del Valle (Tucumn, Argentina).

    PubMed

    Hernndez, N E; Tereschuk, M L; Abdala, L R

    2000-11-01

    Preliminary studies of flavonoids have been realised in five native species from Taf del Valle (Tucumn, Argentina) used in popular medicine. Most of compounds detected were flavonoids mono and dihydroxylated in B ring. Screening for antimicrobial activity against Gram positive and Gram negative microorganisms has been realised with Lippia turbinata, Satureja parvifolia, Sambucus peruviana, Verbena officinalis and Chenopodium graveolens. The total extracts of flavonoids of each plant were tested and four species studied showed antimicrobial activity. PMID:11025172

  12. Un nuevo estudio del cmulo abierto Tr 14 en la regin de Carina

    NASA Astrophysics Data System (ADS)

    Garca, B.; Malaroda, S.; Levato, H.; Morrell, N.

    Presentamos nuevos datos espectroscpicos de 9 objetos entre los miembros ms brillantes de Tr 14. Hemos medido un total de 80 nuevos espectrogramas para contribuir a la determinacin de la real naturaleza de estos objetos desde el punto de vista de la duplicidad. Del nuevo material debemos concluir que la mayora de las estrellas en la muestra son simples. Sin embargo existen algunos objetos cuyo seguimiento debe continuar ya que no nos es posible efectuar conclusiones definitivas con el presente material.

  13. Observacin y registro fotogrfico del cometa HYAKUTAKE C/1996 B2

    NASA Astrophysics Data System (ADS)

    Arquiola, A. P.; Arquiola, F. E.

    Desde el momento en que nos llega la informacin del descubrimiento, comenzamos la observacin de forma detallada a fin de apreciar y seguir su creciente actividad. La primera observacin se realiz el 17 de febrero de 1996 a las 4h20m UT con nuestro telescopio de 300mm newtoniano de f/6.5, con una muy buena transparencia atmosfrica, estimando una magnitud de 8.5. Realizbamos observaciones casi todas las noches, mientras nos lo permitan las condiciones climticas. Ya el 26 de febrero el cometa presentaba un aspecto muy particular, ligeramente distinto a las primeras observaciones, ya que comenzamos a notar su gran actividad. En primer lugar se destacaba la cola de iones mucho mas extensa que la cola de polvo. Magnitud estimada 7.5. El 19 de marzo ya era visible a simple vista y en especial su cola muy abierta y extensa (3 grados) y no quedaba duda alguna que la ltima noche de observacin del cometa en el horizonte de la Ciudad de Funes, que fue el 24 de marzo de 1996, un da antes del acercamiento a la Tierra, tendramos una apreciacin del cometa en su mximo esplendor. El 24 de marzo de 1996 a las 4hs UT, asom el cometa por el horizonte NE, con su cola espectacular, llegando a medir 23 grados de longitud, muy recta y brillante, y su cabellera muy estelar. A este marco de observacin se sumaron mas de 600 personas que participaron usando instrumentos, siendo la mayora de los concurrentes de la vecina Ciudad de Rosario, ya que su observacin era a muy baja atura sobre el horizonte norte. Instrumentos utilizados : Telescopio Newtoniano 300mm f/6.5, Telescopio Schmidt-Cassegrain 200mm , Binoculares 10 x 50, 7 x 50, Lente normal 50mm a f/1.8, Pelcula 100 - 400 ASA Lugar de Observacin : Ciudad de Funes, zona rural, ``Estancia La Polola" Latitud: - 32o 55' Longitud: 60o 40' Oeste

  14. Segundo Catálogo Estelar del Hemisferio Sur con Astrolabio Fotoeléctrico PAII

    NASA Astrophysics Data System (ADS)

    Manrique, W. T.; Podestá, R. C.; Alonso, E.; Actis, E. V.; Pacheco, A. M.; Bustos, G.; Lizhi, L.; Zezhi, W.; Fanmiao, Z.; Hongqi, W.; Perdomo, R.

    Recordamos que entre el Observatorio Astronómico ``Félix Aguilar'', el Observatorio Astronómico de Beijing y el Observatorio Astronómico de La Plata, se ha convenido en desarrollar un Proyecto de Investigación conjunto, para la observación sistemática de estrellas en el Hemisferio Sur, con el objeto de la elaboración de un Catálogo Estelar Global utilizando un Astrolabio Fotoeléctrico PAII del Observatorio de Beijing, que ha sido usado con éxito en la República de China. En este trabajo se presenta el Segundo Catálogo Estelar del Hemisferio Sur, derivado de las observaciones realizadas con el PAII instalado en el OAFA, durante el períiodo Febrero de 1992 a Marzo de 1997. En este lapso se han observado mas de 400000 pasajes estelares, obteniéndose las correcciones Δ α y Δ δ de 5241 estrellas del FK4, FK5, FK5 Ext., SRS, CAMC y GC. Las precisiones medias son del orden de ± 3,2 ms en ascensión recta y ±0."057 en declinación. Rango de magnitudes : 2,0 a 11,5 Rango de declinaciones : -3o a -60o Epoca Media : 1994.9 Se analizan los residuos en función de la magnitud y tipo espectral, correcciones de grupo y frecuencia de distribución Δ α y Δ δ.

  15. Ground Gravity, Magnetic and Electromagnetic Surveys on a Crater on Basalt of Bajada del Diablo Astrobleme-Strewn Field

    NASA Astrophysics Data System (ADS)

    Acevedo, R. D.; Prezzi, C.; Orgeira, M. J.; Rocca, M.; Martnez, O.; Ponce, J. F.; Corbella, H.; Rabassa, J.; Gonzlez-Guillot, M.; Subas, I.

    2014-09-01

    With the aim of further investigate the circular structures from Bajada del Diablo, we carried out geophysics surveys and we conclude that the geophysical features could be satisfactorily explained assuming an extra-terrestrial projectile impact.

  16. Integrated surveillance of pulmonary tuberculosis and paragonimiasis in Zamboanga del Norte, the Philippines

    PubMed Central

    Belizario, Vicente; Totanes, Francis Isidore; Asuncion, Camille Ann; Leon, Winifreda De; Jorge, Manuel; Ang, Concepcion; Naig, June Rose

    2014-01-01

    Background Pulmonary tuberculosis (PTB) and paragonimiasis remain as health problems in certain areas in the Philippines. Both share similar clinical manifestations, which include chronic productive cough, hemoptysis, dyspnea, fever, weight loss, and night sweats. This study aimed to determine the prevalence of PTB, paragonimiasis, and co-infections in Zamboanga del Norte, Philippines. Methods This study was conducted in selected villages in two municipalities in Zamboanga del Norte. Patients with chronic cough were interviewed, examined, and requested to submit two sputum samples which were processed using ZiehlNeelsen method to detect acid-fast bacilli (AFB), and NaOH concentration technique for the detection of Paragonimus ova. Results A total of 836 patients submitted sputum samples for examination. Prevalence was 6.7% (2.512.7%) for paragonimiasis and 1.9% (0.96.3%) for PTB. Co-infection rate was 0.3%, with two identified cases. Positivity rates for males and females were 9.6 and 5.8% for paragonimiasis and 3.4 and 1.2% for PTB. Conclusion Pulmonary tuberculosis and paragonimiasis are co-endemic in Zamboanga del Norte, suggesting the need to integrate surveillance and control efforts. Strengthening local health systems through collaboration between different sectors is recommended for effective disease control. Development of more sensitive diagnostic tests is important for more accurate disease surveillance. PMID:24601907

  17. Experience the magic of light and color: outreach activity by Universidad del Valle student chapter

    NASA Astrophysics Data System (ADS)

    Valdes, Claudia; Reyes, Camilo; Osorio, Alberto; Solarte, Efrain

    2010-08-01

    During 2007, the Universidad del Valle Student Chapter presented a proposal for developing an educational outreach activity for children from an underprivileged zone to the Optical Society of America Foundation (OSAF) and to SPIE. The activity was carried out jointly by OSA and SPIE Universidad del Valle Student Chapters in the hillsides of Santiago de Cali, in a zone known as "Pueblo Joven" during 2008. It was aimed to boys and girls with ages between 8 and 13 years and was called "Experience the magic of light and color". The main purpose was to bring the children some basic concepts on optics and to encourage them to explore science through optics. The Universidad del Valle Student Chapters designed a series of talks and practical workshops where children participated in hands-on experiments that easily explain the fundamental concepts of light phenomena. Afterwards the children presented their achievements in a small science fair offered to the community and tried to explain in their own words what they learned and built. In this work, we present the most successful experimental designs and the educational standards we tried to develop with this activity.

  18. Agregacin de para-H2 en chorros supersnicos: del dmero al slido

    NASA Astrophysics Data System (ADS)

    Mara Fernndez, Jos

    En este trabajo se mostrarn los estudios de expansiones supersnicas de para-H2 desde toberas criognicas, realizados en el laboratorio de Fluidodinmica Molecular del IEM. Hemos registrado los espectros Raman de dichas expansiones en el intervalo de frecuencias de 4145 a 4162 cm-1, prximo a la lnea Q(0) del monmero no rotante H2, a 4161.18 cm-1. En el espectro Raman, adems de la lnea Q(0) del monmero, se observan unos picos dbiles, que, por su comportamiento con la temperatura de la tobera y la distancia a la misma, asignamos a la transicin homloga en agregados de p-H2. Dependiendo de las condiciones, se observan agregados pequeos (de 2 a 13 molculas de p-H2), agregados medios y masivos, y se llega incluso al lquido y al slido. Las asignaciones se apoyan en un modelo sencillo, basado en la perturbacin, debida al potencial intermolecular, de los estados vibracionales v=0 y v=1 en los agregados de p-H2. Se mostrarn detalles de la instalacin experimental, ejemplos de los espectros obtenidos en diversas condiciones, y detalles de la interpretacin cuantitativa.

  19. Variation in mangrove forest structure and sediment characteristics in Bocas del Toro, Panama

    USGS Publications Warehouse

    Lovelock, C.E.; Feller, Ilka C.; McKee, K.L.; Thompson, R.

    2005-01-01

    Mangrove forest structure and sediment characteristics were examined in the extensive mangroves of Bocas del Toro, Republic of Panama. Forest structure was characterized to determine if spatial vegetation patterns were repeated over the Bocas del Toro landscape. Using a series of permanent plots and transects we found that the forests of Bocas del Toro were dominated by Rhizophora mangle with very few individuals of Avicennia germinans and Laguncularia racemosa. Despite this low species diversity, there was large variation in forest structure and in edaphic conditions (salinity, concentration of available phosphorus, Eh and sulphide concentration). Aboveground biomass varied 20-fold, from 6.8 Mg ha-1 in dwarf forests to 194.3 Mg ha-1 in the forests fringing the land. But variation in forest structure was predictable across the intertidal zone. There was a strong tree height gradient from seaward fringe (mean tree height 3.9 m), decreasing in stature in the interior dwarf forests (mean tree height 0.7 m), and increasing in stature in forests adjacent to the terrestrial forest (mean tree height 4.1 m). The predictable variation in forest structure emerges due to the complex interactions among edaphic and plant factors. Identifying predictable patterns in forest structure will aid in scaling up the ecosystem services provided by mangrove forests in coastal landscapes. Copyright 2005 College of Arts and Sciences.

  20. Observaciones del disco solar y de una protuberancia quiescente en radiación ultravioleta

    NASA Astrophysics Data System (ADS)

    Cirigliano, D.; Vial, J.-C.; Rovira, M.

    Observaciones del disco solar y de una protuberancia quiescente en el rango de longitudes de onda ultravioleta fueron obtenidas con el instrumento CDS (Coronal Diagnostic Spectrograph) y SUMER (Solar Ultraviolet Measurements of emitted radiation) a bordo de la sonda SOHO. El propósito es investigar las velocidades macroscópicas de varias especies metálicas que se observan tanto en el disco solar como en el plasma de las protuberancias. Para calcular las velocidades del disco solar aplicamos una técnica mixta para modelar la distribución de estructuras en UV en el Sol quieto. Las velocidades macroscópicas en las protuberancias se calcularon a partir de los corrimientos Doppler en cada línea espectral y luego se tomaron las del disco solar como referencia. Obtuvimos valores absolutos para las velocidades macroscópicas entre 5 y 40 km/seg. También detectamos comportamientos diferentes en las velocidades de las protuberancias en el centro con respecto a los bordes.

  1. Campano-Maastrichtian foraminifera from onshore sediments in the Rio del Rey Basin, Southwest Cameroon

    NASA Astrophysics Data System (ADS)

    Njoh, Oliver Anoh; Victor, Obiosio; Christopher, Agyingi

    2013-03-01

    Campanian-Maastrichtian marine sediments outcrop in five genetically linked sedimentary basins along the West African coast in the Gulf of Guinea, from the Douala Basin in Cameroon to the Anambra Basin in Nigeria. These sediments in the more centrally located Rio del Rey Basin have been the least studied. Therefore, the geologic history of this region has merely been speculative. The Rio del Rey Basin like the adjacent Niger Delta is producing hydrocarbon from the offshore Tertiary sedimentary interval in which all studies have been focused, neglecting the onshore Cretaceous sediments. Outcrops in the basin are rare, small and highly weathered. Samples from some of these sediments have yielded a few Planktonic and dominantly benthonic foraminiferal assemblages. The long-ranging heterohelix and hedbergellids characterized the planktics while the species Afrobolivina afra which is a well known diagnostic taxon for Campanian-Maastrichtian sediments in West African basins clearly dominate the benthic assemblage. Its occurrence in association with other Upper Cretaceous forms such as Bolivina explicata, Praebulimina exiqua, Gabonita lata, Ammobaculites coprolithiformis amongst others, formed the basis on which this age was assigned to the sediments sampled from the Rio del Rey Basin. Hence, this work has undoubtedly established the much needed link in this regional geologic history and correlates these sediments with the Logbaba and Nkporo Formations in the Douala Basin in Cameroon and the southeastern Nigerian Sedimentary Basins. Thus, these units were all deposited during this same geologic period and probably controlled by the same geologic event.

  2. Lenalidomide induces ubiquitination and degradation of CK1? in del(5q) MDS.

    PubMed

    Krnke, Jan; Fink, Emma C; Hollenbach, Paul W; MacBeth, Kyle J; Hurst, Slater N; Udeshi, Namrata D; Chamberlain, Philip P; Mani, D R; Man, Hon Wah; Gandhi, Anita K; Svinkina, Tanya; Schneider, Rebekka K; McConkey, Marie; Jrs, Marcus; Griffiths, Elizabeth; Wetzler, Meir; Bullinger, Lars; Cathers, Brian E; Carr, Steven A; Chopra, Rajesh; Ebert, Benjamin L

    2015-07-01

    Lenalidomide is a highly effective treatment for myelodysplastic syndrome (MDS) with deletion of chromosome 5q (del(5q)). Here, we demonstrate that lenalidomide induces the ubiquitination of casein kinase 1A1 (CK1?) by the E3 ubiquitin ligase CUL4-RBX1-DDB1-CRBN (known as CRL4(CRBN)), resulting in CK1? degradation. CK1? is encoded by a gene within the common deleted region for del(5q) MDS and haploinsufficient expression sensitizes cells to lenalidomide therapy, providing a mechanistic basis for the therapeutic window of lenalidomide in del(5q) MDS. We found that mouse cells are resistant to lenalidomide but that changing a single amino acid in mouse Crbn to the corresponding human residue enables lenalidomide-dependent degradation of CK1?. We further demonstrate that minor side chain modifications in thalidomide and a novel analogue, CC-122, can modulate the spectrum of substrates targeted by CRL4(CRBN). These findings have implications for the clinical activity of lenalidomide and related compounds, and demonstrate the therapeutic potential of novel modulators of E3 ubiquitin ligases. PMID:26131937

  3. Proteolytic effect of Cynara cardunculus rennet for use in the elaboration of 'Torta del Casar' cheese.

    PubMed

    Ordiales, Elena; Benito, Maria Jos; Martin, Alberto; Fernndez, Margarita; Hernndez, Alejandro; de Guia Crdoba, Maria

    2013-11-01

    The purpose of this work was to analyse the influence of rennet from different Cynara cardunculus plants, selected for its clotting and proteolytic activity on caseins, on the characteristics of manufactured 'Torta del Casar' cheeses. After classifying the cardoon according to proteolytic activity into five groups of greater or lesser activity, 16 batches of cheeses were made with rennet derived from different wild cardoon plants. We observed a major development of the proteolysis during ripening leading to the generation of non-protein nitrogen compounds. Especially noteworthy was the relationship of amino acid nitrogen (AN) generation with rennet clotting activity after 24 h of maceration, and the fact that the production of biogenic amines was not related to the proteolytic activity of the rennet. The activities of the rennet observed 'in vitro' were also developed 'in vivo' in the cheeses, with the different rennets used affecting the final sensory characteristics of cheeses. The rennet with high clotting activity after 24 h of maceration was positively correlated with the creaminess, viscosity, and acceptability of the cheese. However, the high proteolytic activity rennet negatively influenced the acidity, bitterness, and creaminess parameters. Therefore the most appropriate cardoons for making this cheese are those with higher clotting activities and moderate proteolytic activities especially on ?-casein. The use of controlled and characterised cardoons in the manufacturing process of Torta del Casar is fundamental to obtaining the homogeneous product demanded by the Torta del Casar Registry of the Protected Designation of Origin. PMID:24063288

  4. Lenalidomide for myelodysplastic syndromes with del(5q): how long should it last?

    PubMed

    Vozella, Federico; Latagliata, Roberto; Carmosino, Ida; Volpicelli, Paola; Montagna, Chiara; Romano, Angela; Roberto, Amanda; Finsinger, Paola; Mancini, Marco; Breccia, Massimo; Oliva, Esther; Oliva, Esther

    2015-03-01

    Lenalidomide induces in patients with myelodysplastic syndrome (MDS) and del(5q) erythroid and cytogenetic response rates as high as 75% and 50%, respectively. It is still unclear, however, how long lenalidomide treatment should be continued and whether or not the drug could be interrupted. To assess the feasibility of lenalidomide discontinuation, we revised a cohort of 16 low-risk MDS patients with del(5q) treated at our institute in a phase II multicentric Italian study. Among the 12 responding patients, four discontinued lenalidomide while in complete response. All four patients needed during treatment a permanent lenalidomide reduction from 10 to 5?mg/day because of haematological toxicity (three patients) or grade 3 muscular and bone pain (one patient). At lenalidomide discontinuation after 16, 20, 27 and 20?months from the start, respectively, all four patients were in complete hematologic response and three forth in complete cytogenetic response. Three patients are still in response after 36, 30 and 20?months from lenalidomide discontinuation, respectively: The remaining patient relapsed after 20?months, and she is now receiving a new course of lenalidomide. In conclusion, long-lasting remissions are achievable in MDS patients with del(5q) in complete response after lenalidomide discontinuation. PMID:25950027

  5. A Precambrian cratonic block in the west-central Chihuahua - The Sierra del Nido cratonic block

    SciTech Connect

    Goodell, P.C. . Dept. of Geological)

    1993-02-01

    Precambrian rocks in west-central Chihuahua have been recognized by Denison (1969) and Mauger et al. (1983), on the basis of radiometric dating. The rocks are rhyolite clasts, and an allucthonous block, respectively, however their source direction and vergence can be measured. They point back to and are on the edge of a large, uniform, negative Bouguer gravity anomaly, having values greater than 200 milligals. The isotopic geochemical character of several Tertiary felsic fields within this anomalous are has been determined, and initial strontium isotopic ratios are all greater than 0.7055. Outside the anomalous area these ratios are lower, and Basin and Range extension tectonism is more evident. It is proposed that a Precambrian cratonic block, the Sierra del Nido, is present in the crust in west-central Chihuahua. It is reasonable to propose that it was decreted from North America during a Precambrian extensional (1.1. By ) event, from somewhere along the Arizona Transition Zone-Texas Linament region. The Sierra del Nido Block is separated form the ATZ-TL by a region of disrupted craton and extended crust, the Basin and Range Province. Implications of the pressure of the Sierra del Nido Block on other regional tectonic events will be discussed.

  6. La reconstrucción del seno después de una mastectomía

    Cancer.gov

    Hoja informativa que describe la reconstrucción del seno (mama) después de una mastectomía. Contiene información sobre las opciones quirúrgicas, los cuidados de seguimiento y los exámenes de detección del cáncer de seno después de la reconstrucción.

  7. ∆ DNMT3B4-del Contributes to Aberrant DNA Methylation Patterns in Lung Tumorigenesis

    PubMed Central

    Ma, Mark Z.; Lin, Ruxian; Carrillo, José; Bhutani, Manisha; Pathak, Ashutosh; Ren, Hening; Li, Yaokun; Song, Jiuzhou; Mao, Li

    2015-01-01

    Aberrant DNA methylation is a hallmark of cancer but mechanisms contributing to the abnormality remain elusive. We have previously shown that ∆DNMT3B is the predominantly expressed form of DNMT3B. In this study, we found that most of the lung cancer cell lines tested predominantly expressed DNMT3B isoforms without exons 21, 22 or both 21 and 22 (a region corresponding to the enzymatic domain of DNMT3B) termed DNMT3B/∆DNMT3B-del. In normal bronchial epithelial cells, DNMT3B/ΔDNMT3B and DNMT3B/∆DNMT3B-del displayed equal levels of expression. In contrast, in patients with non-small cell lung cancer NSCLC), 111 (93%) of the 119 tumors predominantly expressed DNMT3B/ΔDNMT3B-del, including 47 (39%) tumors with no detectable DNMT3B/∆DNMT3B. Using a transgenic mouse model, we further demonstrated the biological impact of ∆DNMT3B4-del, the ∆DNMT3B-del isoform most abundantly expressed in NSCLC, in global DNA methylation patterns and lung tumorigenesis. Expression of ∆DNMT3B4-del in the mouse lungs resulted in an increased global DNA hypomethylation, focal DNA hypermethylation, epithelial hyperplastia and tumor formation when challenged with a tobacco carcinogen. Our results demonstrate ∆DNMT3B4-del as a critical factor in developing aberrant DNA methylation patterns during lung tumorigenesis and suggest that ∆DNMT3B4-del may be a target for lung cancer prevention. PMID:26629529

  8. Estimaciones de Prevalencia del VIH por Género y Grupo de Riesgo en Tijuana, México: 2006

    PubMed Central

    Iñiguez-Stevens, Esmeralda; Brouwer, Kimberly C.; Hogg, Robert S.; Patterson, Thomas L.; Lozada, Remedios; Magis-Rodriguez, Carlos; Elder, John P.; Viani, Rolando M.; Strathdee, Steffanie A.

    2010-01-01

    OBJETIVO Estimar la prevalencia del VIH en adultos de 15-49 años de edad en Tijuana, México - en la población general y en subgrupos de riesgo en el 2006. METODOS Se obtuvieron datos demográficos del censo Mexicano del 2005, y la prevalencia del VIH se obtuvo de la literatura. Se construyó un modelo de prevalencia del VIH para la población general y de acuerdo al género. El análisis de sensibilidad consistió en estimar errores estándar del promedio-ponderado de la prevalencia del VIH y tomar derivados parciales con respecto a cada parámetro. RESULTADOS La prevalencia del VIH es 0.54%(N = 4,347) (Rango: 0.22%–0.86%, (N = 1,750–6,944)). Esto sugiere que 0.85%(Rango: 0.39%–1.31%) de los hombres y 0.22%(Rango: 0.04%–0.40%) de las mujeres podrían ser VIH-positivos. Los hombres que tienen sexo con hombres (HSH), las trabajadoras sexuales usuarias de drogas inyectables (MTS-UDI), MTS-noUDI, mujeres UDI, y los hombres UDI contribuyeron las proporciones más elevadas de personas infectadas por el VIH. CONCLUSIONES El número de adultos VIH-positivos entre subgrupos de riesgo en la población de Tijuana es considerable, marcando la necesidad de enforcar las intervenciones de prevención en sus necesidades específicas. El presente modelo estima que hasta 1 en cada 116 adultos podrían ser VIH-positivos. PMID:19685824

  9. Identification and functional characterisation of a novel KCNJ2 mutation, Val302del, causing Andersen-Tawil syndrome.

    PubMed

    rdg, Balzs; Hategan, Lidia; Kovcs, Mria; Seprnyi, Gyrgy; Kohajda, Zsfia; Nagy, Istvn; Heged?s, Zoltn; Krnyei, Lszl; Jost, Norbert; Katona, Mrta; Szekeres, Mikls; Forster, Tams; Papp, Julius Gy; Varr, Andrs; Sepp, Rbert

    2015-07-01

    Loss-of-function mutations of the KCNJ2 gene encoding for the inward rectifier potassium channel subunit Kir2.1 cause Andersen-Tawil Syndrome (ATS), a rare genetic disorder characterised by periodic paralysis, ventricular arrhythmias, and dysmorphic features. Clinical manifestations of the disease appear to vary greatly with the nature of mutation, therefore, functional characterisation of ATS-causing mutations is of clinical importance. In this study, we describe the identification and functional analysis of a novel KCNJ2 mutation, Val302del, identified in a patient with ATS. Heterologously expressed wild type (WT) and Val302del mutant alleles showed similar subcellular distribution of the Kir2.1 protein with high intensity labelling from the membrane region, demonstrating normal membrane trafficking of the Val302del Kir2.1 variant. Cells transfected with the WT allele displayed a robust current with strong inward rectification, while no current above background was detected in cells expressing the Val302del Kir2.1 subunit. Co-transfection of CHO cells with the WT and the Val302del Kir2.1 revealed a dose-dependent inhibitory effect of the Val302del Kir2.1 mutant subunit on WT Kir2.1 currents. These observations indicate that the WT and the Val302del mutant subunits co-assemble in the cell membrane and that the mutation affects potassium conductivity and (or) gating of the WT/Val302del heteromeric Kir2.1 channels. PMID:26103554

  10. DEL RBC transfusion should be avoided in particular blood recipient in East Asia due to allosensitization and ineffectiveness*

    PubMed Central

    Shao, Chao-peng; Wang, Bao-yan; Ye, Shi-hui; Zhang, Wen-li; Xu, Hua; Zhuang, Nai-bao; Wu, Xiao-ying; Xu, Heng-gui

    2012-01-01

    Previously, both primary and secondary anti-D alloimmunizations induced by Asian type DEL (RHD1227A allele) were observed in two incidents. We investigated how often these alloimmunization events occur. The transfusions of any D-negative patients were investigated in the First Affiliated Hospital of Xian Jiaotong University Medical College, China, during the entire 2009. The antigens of D, C, c, E, and e were routinely serotyped. The Asian type DEL variant was genotyped and the RHD heterozygote was determined through two published methods. The changes in anti-D levels were monitored by the indirect antiglobulin test (IAT) and flow cytometry. Thirty D-negative transfused patients were included in the study. We focused on 11 recipients who were transfused with packed red blood cells (RBCs) from DEL donors at least one time. Of those 11 recipients, seven were anti-D negative before transfusion and four were anti-D positive (one patient with an autoantibody). One of the seven pre-transfusion anti-D negative patients produced a primary-response anti-D after being transfused with 400 ml of DEL blood twice. All four pre-transfusion antibody positive patients were not observed hemoglobin (Hb) levels increased, as expected after transfusions. Two patients had an increase in anti-D from 1:8 to 1:64 by IAT, which was also shown by flow cytometry. None of the patients experienced an acute hemolytic episode. Our data indicated that the primary anti-D induced by DEL transfusion or the secondary anti-D elevated by DEL in a truly D-negative patient might not be unusual. We suggest that a truly D-negative childbearing-aged woman should avoid DEL transfusion to protect her from primary anti-D allosensitization. In addition, anti-D positive recipients should also avoid DEL red cell transfusion due to the delayed hemolytic transfusion reaction (DHTR). PMID:23125084

  11. Correction of the F508del-CFTR protein processing defect in vitro by the investigational drug VX-809

    PubMed Central

    Van Goor, Fredrick; Hadida, Sabine; Grootenhuis, Peter D. J.; Burton, Bill; Stack, Jeffrey H.; Straley, Kimberly S.; Decker, Caroline J.; Miller, Mark; McCartney, Jason; Olson, Eric R.; Wine, Jeffrey J.; Frizzell, Ray A.; Ashlock, Melissa; Negulescu, Paul A.

    2011-01-01

    Cystic fibrosis (CF) is caused by mutations in the CF transmembrane conductance regulator (CFTR) gene that impair the function of CFTR, an epithelial chloride channel required for proper function of the lung, pancreas, and other organs. Most patients with CF carry the F508del CFTR mutation, which causes defective CFTR protein folding and processing in the endoplasmic reticulum, resulting in minimal amounts of CFTR at the cell surface. One strategy to treat these patients is to correct the processing of F508del-CFTR with small molecules. Here we describe the in vitro pharmacology of VX-809, a CFTR corrector that was advanced into clinical development for the treatment of CF. In cultured human bronchial epithelial cells isolated from patients with CF homozygous for F508del, VX-809 improved F508del-CFTR processing in the endoplasmic reticulum and enhanced chloride secretion to approximately 14% of non-CF human bronchial epithelial cells (EC50, 81 ± 19 nM), a level associated with mild CF in patients with less disruptive CFTR mutations. F508del-CFTR corrected by VX-809 exhibited biochemical and functional characteristics similar to normal CFTR, including biochemical susceptibility to proteolysis, residence time in the plasma membrane, and single-channel open probability. VX-809 was more efficacious and selective for CFTR than previously reported CFTR correctors. VX-809 represents a class of CFTR corrector that specifically addresses the underlying processing defect in F508del-CFTR. PMID:21976485

  12. Role of additional chromosomal changes in the prognostic value of t(4;14) and del(17p) in multiple myeloma: the IFM experience.

    PubMed

    Hebraud, Benjamin; Magrangeas, Florence; Cleynen, Alice; Lauwers-Cances, Valerie; Chretien, Marie-Lorraine; Hulin, Cyrille; Leleu, Xavier; Yon, Edwige; Marit, Gerald; Karlin, Lionel; Roussel, Murielle; Stoppa, Anne-Marie; Belhadj, Karim; Voillat, Laurent; Garderet, Laurent; Macro, Margaret; Caillot, Denis; Mohty, Mohamad; Facon, Thierry; Moreau, Philippe; Attal, Michel; Munshi, Nikhil; Corre, Jill; Minvielle, Stephane; Avet-Loiseau, Herve

    2015-03-26

    In multiple myeloma, cytogenetic changes are important predictors of patient outcome. In this setting, the most important changes are deletion 17p, del(17p), and translocation of chromosomes 4 and 14, t(4;14), conferring a poor outcome. However, a certain degree of heterogeneity is observed in the survival of these high-risk patients. We hypothesized that other chromosomal changes may impact the outcome. We retrospectively analyzed a large series of 242 patients displaying either t(4;14) (157 patients) or del(17p) (110 patients), 25 patients presenting both abnormalities, using single nucleotide polymorphism array. In patients with t(4;14), del(1p32), del22q, and >30 chromosomal structural changes negatively impacted progression-free survival (PFS). For overall survival (OS), del(13q14), del(1p32), and the number of chromosomal structural changes worsened the prognosis of patients. For patients with del(17p), del6q worsened the prognosis of patients, whereas trisomy 15 and monosomy 14 were found to have a protective effect on PFS. For OS, del(1p32) worsened the prognosis of patients, whereas having >8 numerical changes was found to have a protective effect on survival. This study, which is the largest series of high-risk patients analyzed with the most modern genomic technique, identified 1 main factor negatively impacting survival: del(1p32). PMID:25636340

  13. Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3β-C/EBPβ pathway.

    PubMed

    Maekawa, Tomoki; Hosur, Kavita; Abe, Toshiharu; Kantarci, Alpdogan; Ziogas, Athanasios; Wang, Baomei; Van Dyke, Thomas E; Chavakis, Triantafyllos; Hajishengallis, George

    2015-01-01

    Del-1 is an endothelial cell-secreted anti-inflammatory protein. In humans and mice, Del-1 expression is inversely related to that of IL-17, which inhibits Del-1 through hitherto unidentified mechanism(s). Here we show that IL-17 downregulates human endothelial cell expression of Del-1 by targeting a critical transcription factor, C/EBPβ. Specifically, IL-17 causes GSK-3β-dependent phosphorylation of C/EBPβ, which is associated with diminished C/EBPβ binding to the Del-1 promoter and suppressed Del-1 expression. This inhibitory action of IL-17 can be reversed at the GSK-3β level by PI3K/Akt signalling induced by D-resolvins. The biological relevance of this regulatory network is confirmed in a mouse model of inflammatory periodontitis. Intriguingly, resolvin-D1 (RvD1) confers protection against IL-17-driven periodontal bone loss in a Del-1-dependent manner, indicating an RvD1-Del-1 axis against IL-17-induced pathological inflammation. The dissection of signalling pathways regulating Del-1 expression provides potential targets to treat inflammatory diseases associated with diminished Del-1 expression, such as periodontitis and multiple sclerosis. PMID:26374165

  14. Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3β-C/EBPβ pathway

    PubMed Central

    Maekawa, Tomoki; Hosur, Kavita; Abe, Toshiharu; Kantarci, Alpdogan; Ziogas, Athanasios; Wang, Baomei; Van Dyke, Thomas E.; Chavakis, Triantafyllos; Hajishengallis, George

    2015-01-01

    Del-1 is an endothelial cell-secreted anti-inflammatory protein. In humans and mice, Del-1 expression is inversely related to that of IL-17, which inhibits Del-1 through hitherto unidentified mechanism(s). Here we show that IL-17 downregulates human endothelial cell expression of Del-1 by targeting a critical transcription factor, C/EBPβ. Specifically, IL-17 causes GSK-3β-dependent phosphorylation of C/EBPβ, which is associated with diminished C/EBPβ binding to the Del-1 promoter and suppressed Del-1 expression. This inhibitory action of IL-17 can be reversed at the GSK-3β level by PI3K/Akt signalling induced by D-resolvins. The biological relevance of this regulatory network is confirmed in a mouse model of inflammatory periodontitis. Intriguingly, resolvin-D1 (RvD1) confers protection against IL-17-driven periodontal bone loss in a Del-1-dependent manner, indicating an RvD1-Del-1 axis against IL-17-induced pathological inflammation. The dissection of signalling pathways regulating Del-1 expression provides potential targets to treat inflammatory diseases associated with diminished Del-1 expression, such as periodontitis and multiple sclerosis. PMID:26374165

  15. 500 days of Stromgren b, y and narrow-band [OIII], H ? photometric evolution of gamma-ray Nova Del 2013 (=V339 Del)

    NASA Astrophysics Data System (ADS)

    Munari, Ulisse; Maitan, Alessandro; Moretti, Stefano; Tomaselli, Salvatore

    2015-10-01

    We present and discuss highly accurate photometry obtained through medium Stromgren y, b bands and narrow [OIII], H? bands covering 500 days of the evolution of Nova Del 2013 since its maximum brightness. This is by far the most complete study of any nova observed in such photometric systems. The nova behavior in these photometric bands is very different from that observed with the more conventional broad bands like UBVRC IC or u?g?r?i?z? , providing unique information about extent and ionization of the ejecta, the onset of critical phases like the transition between optically thick and thin conditions, and re-ionization by the central super-soft X-ray source. The actual transmission profiles of the y, b , [OIII] and H? photometric filters have been accurately measured at different epochs and different illumination angles, to evaluate in detail their performance under exact operating conditions. The extreme smoothness of both the H? and [OIII] lightcurves argues for absence of large and abrupt discontinuities in the ejecta of Nova Del 2013. Should they exist, glitches in the lightcurves would have appeared when the ionization and/or recombination fronts overtook them. During the period of recorded very large variability (up to 100 over a single day) in the super-soft X-ray emission (from day +69 to +86 past V maximum), no glitch in excess of 1% was observed in the optical photometry, either in the continuum (Stromgren y) or in the lines ([OIII] and H? filters), or in a combination of the two (Stromgren b, Johnson B and V). Considering that the recombination time scale in the ejecta was one week at that time, this excludes global changes of the white dwarf as the source of the X-ray variability and supports instead clumpy ejecta passing through the line of sight to us as its origin.

  16. Restoration of CFTR function in patients with cystic fibrosis carrying the F508del-CFTR mutation

    PubMed Central

    Stefano, Daniela De; Villella, Valeria R; Esposito, Speranza; Tosco, Antonella; Sepe, Angela; Gregorio, Fabiola De; Salvadori, Laura; Grassia, Rosa; Leone, Carlo A; Rosa, Giuseppe De; Maiuri, Maria C; Pettoello-Mantovani, Massimo; Guido, Stefano; Bossi, Anna; Zolin, Anna; Venerando, Andrea; Pinna, Lorenzo A; Mehta, Anil; Bona, Gianni; Kroemer, Guido; Maiuri, Luigi; Raia, Valeria

    2014-01-01

    Restoration of BECN1/Beclin 1-dependent autophagy and depletion of SQSTM1/p62 by genetic manipulation or autophagy-stimulatory proteostasis regulators, such as cystamine, have positive effects on mouse models of human cystic fibrosis (CF). These measures rescue the functional expression of the most frequent pathogenic CFTR mutant, F508del, at the respiratory epithelial surface and reduce lung inflammation in CftrF508del homozygous mice. Cysteamine, the reduced form of cystamine, is an FDA-approved drug. Here, we report that oral treatment with cysteamine greatly reduces the mortality rate and improves the phenotype of newborn mice bearing the F508del-CFTR mutation. Cysteamine was also able to increase the plasma membrane expression of the F508del-CFTR protein in nasal epithelial cells from F508del homozygous CF patients, and these effects persisted for 24h after cysteamine withdrawal. Importantly, this cysteamine effect after washout was further sustained by the sequential administration of epigallocatechin gallate (EGCG), a green tea flavonoid, both in vivo, in mice, and in vitro, in primary epithelial cells from CF patients. In a pilot clinical trial involving 10 F508del-CFTR homozygous CF patients, the combination of cysteamine and EGCG restored BECN1, reduced SQSTM1 levels and improved CFTR function from nasal epithelial cells in vivo, correlating with a decrease of chloride concentrations in sweat, as well as with a reduction of the abundance of TNF/TNF-alpha (tumor necrosis factor) and CXCL8 (chemokine [C-X-C motif] ligand 8) transcripts in nasal brushing and TNF and CXCL8 protein levels in the sputum. Altogether, these results suggest that optimal schedules of cysteamine plus EGCG might be used for the treatment of CF caused by the F508del-CFTR mutation. PMID:25350163

  17. The Torres del Paine intrusion as a model for a shallow magma chamber

    NASA Astrophysics Data System (ADS)

    Baumgartner, Lukas; Bodner, Robert; Leuthold, Julien; Muntener, Othmar; Putlitz, Benita; Vennemann, Torsten

    2014-05-01

    The shallow magmatic Torres del Paine Intrusive Complex (TPIC) belongs to a series of sub-volcanic and plutonic igneous bodies in Southern Chile and Argentina. This trench-parallel belt is located in a transitional position between the Patagonia Batholith in the West, and the alkaline Cenozoic plateau lavas in the East. While volumetrically small amounts of magmatism started around 28 my ago in the Torres del Paine area, and a second period occurred between 17-16 Ma, it peaked with the TPIC 12.59-12.43 Ma ago. The spectacular cliffs of the Torres del Paine National park provide a unique opportunity to study the evolution of a very shallow magma chamber and the interaction with its host rocks. Intrusion depth can be estimated based on contact metamorphic assemblages and granite solidus thermobarometry to 750250 bars, corresponding to an intrusion depth of ca. 3km, ca. 500m above the base of the intrusion. Hornblende thermobarometry in mafic rocks agrees well with these estimates (Leuthold et al., 2014). The TPIC is composed of a granitic laccolith emplaced over 90ka (Michel et al., 2008) in 3 major, several 100m thick sheets, forming an overall thickness of nearly 2 km. Contacts are sharp between sheets, with the oldest sheet on the top and the youngest on the bottom (Michel et al., 2008). The granitic laccolith is under-plated by a ca. 400m thick mafic laccolith, built up over ca. 50ka (Leuthold et al. 2012), constructed from the bottom up. Granitic and mafic sheets are themselves composed of multiple metric to decametric pulses, mostly with ductile contacts between them, resulting in outcrop patterns resembling braided stream sediments. The contact of the TPIC with the Cretaceous flysch sediments document intrusion mechanism. Pre-existing sub-horizontal fold axes are rotated in the roof of the TPIC, clearly demonstrating ballooning of the roof; no ballooning was observed in the footwall of the intrusion. Extension during ballooning of the roof is indicated by dykes emanating from the roof of the intrusion into the host rocks. The dykes are perpendicular to the contact, radially shooting into the country rocks. Since the oldest granite is found in the roof, it is also permeated by dykes of successive intrusions. Contact metamorphism can be used to constrain the granite intrusion temperature to ca. 1000C. Intrusion occurred in multiple pulses along the granite-host rock contact, in rapid succession, before significant cooling occurred in the aureole. Hydration of the biotite and feldspar of the immature sediments in the outer aureole contributed significantly to the overall thermal signature of the host-rock-TPIC system. In contrast, stable isotopes do not document significant fluid circulation. Leuthold, J., Mntener, O., Baumgartner, L., Putlitz, B., Ovtcharova, M., Schaltegger, U. (2012) Time resolved construction of a granitic to mafic laccolith (Torres del Paine, Patagonia). Earth Plan Sci Lett. 325. 85-92 Leuthold J., Mntener O., Baumgartner L.P., Putlitz B., (2014) Recycling of mafic crystal mush, transport and emplacement in the Torres del Paine mafic complex (Patagonia). J. Petrology; accepted pending revision Michel, J., Baumgartner, L.P. Putlitz, B., Ovtcharova, M., Schaltegger, U. (2008) Incremental growth of a shallow crustal laccolith over 80 kyrs: the Torres del Paine Granite, Patagonia. Geology vol. 36, 459-462

  18. NFKB1 -94ins/del ATTG polymorphism increases osteosarcoma risk in a Chinese Han population.

    PubMed

    Li, Xiyi; Zhang, Chenglin; Qiao, Wei; Zhou, Xuhui; Sun, Menghong

    2015-01-01

    Osteosarcoma is one of the most common bone malignancies. The Nuclear factor-?B1 (NFKB1) gene plays an important role in the pathogenesis of osteosarcoma. The objective of this study aimed to detect the potential association between NFKB1 -94 ins/del ATTG polymorphism and osteosarcoma susceptibility in Chinese Han population. We recruited 220 osteosarcoma patients and 222 cancer-free controls in this case-control study. The NFKB1 -94 ins/del ATTG polymorphism by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Patients with ID genotype and II genotype showed higher risk of osteosarcoma than individuals with DD genotype (OR=1.54, 95% CI 1.00-2.44, P=0.05; OR=1.72, 95% CI 1.01-2.93, P=0.04), respectively. Subjects with ID or II genotype also showed increased risk of osteosarcoma (OR=1.60, 95% CI 1.04-2.47, P=0.03). In addition, I allele was significantly associated with osteosarcoma risk (OR=1.31, 95% CI 1.01-1.71, P=0.04). We also found that this polymorphism was significantly associated with advanced osteosarcoma risk (OR=3.43, 95% CI 1.61-7.36, P=0.001) and metastatic osteosarcoma risk (OR=2.33, 95% CI 1.22-5.03, P=0.01). In conclusion, our findings indicate that osteosarcoma is associated with the NFKB1 promoter -94ins/del ATTG polymorphism. PMID:25785149

  19. Paleomagnetic evidence for an episodic eruptive history of the Cerros del Rio volcanic field, New Mexico

    NASA Astrophysics Data System (ADS)

    Hudson, M. R.; Thompson, R. A.

    2011-12-01

    The Pliocene to Quaternary (~2.6-1.14 Ma) Cerros del Rio volcanic field of northern New Mexico forms a dissected basaltic plateau sourced by multiple eruptive centers. Paleomagnetic data compliment geologic mapping, geochronologic and geochemical data to define the spatial and temporal eruptive history of Cerros del Rio volcanic deposits. The preserved stratigraphic sequence reflects three principal phases of volcanism; 1) 2.7-2.6 Ma, 2) 2.5-2.2 Ma, and 3) 1.5-1.1 Ma. Paleomagnetic data collected from 85 sites that span the area of the volcanic field largely sample phase-1 deposits that record the Guass normal-polarity chron or phase-2 deposits that record the Matuyama reversed-polarity chron. A grand mean of individual sites (excluding transitional directions) is D = 352.8, I = 49.7, k= 14, a95 = 3.9. However, normal- and reversed-polarity group means are not statistically antipodal, with the normal-polarity inclination being significantly shallower than an expected (55) dipole inclination. This failed reversal test suggests that paleosecular variation has not be fully averaged within both polarity groups, despite a basis on abundant data from multiple eruptive centers. Compared to variation recorded by the full volcanic field, site directions from individual eruptive centers have restricted dispersion, indicating that the centers formed quickly relative to paleosecular variation. Grouping data within individual eruptive centers to calculate eruptive-group means (EGM), directions of the normal- and reversed-polarity EGM remain skewed from antipodal. Modal analysis demonstrates the presence of multiple directional clusters among the normal-polarity EGM whereas the frequency distribution of reversed polarity EGM are symmetrical about their maximum. These paleomagnetic directional characteristics indicate that voluminous phase-1 deposits of the Cerros del Rio volcanic field probably erupted episodically during short time intervals and that several individual volcanic centers were erupted nearly synchronously.

  20. Biophysical Characterisation of Calumenin as a Charged F508del-CFTR Folding Modulator

    PubMed Central

    Tripathi, Rashmi; Benz, Nathalie; Culleton, Bridget; Trouv, Pascal; Frec, Claude

    2014-01-01

    The cystic fibrosis transmembrane regulator (CFTR) is a cyclic-AMP dependent chloride channel expressed at the apical surface of epithelial cells lining various organs such as the respiratory tract. Defective processing and functioning of this protein caused by mutations in the CFTR gene results in loss of ionic balance, defective mucus clearance, increased proliferation of biofilms and inflammation of human airways observed in cystic fibrosis (CF) patients. The process by which CFTR folds and matures under the influence of various chaperones in the secretory pathway remains incompletely understood. Recently, calumenin, a secretory protein, belonging to the CREC family of low affinity calcium binding proteins has been identified as a putative CFTR chaperone whose biophysical properties and functions remain uncharacterized. We compared hydropathy, instability, charge, unfoldability, disorder and aggregation propensity of calumenin and other CREC family members with CFTR associated chaperones and calcium binding proteins, wild-type and mutant CFTR proteins and intrinsically disordered proteins (IDPs). We observed that calumenin, along with other CREC proteins, was significantly more charged and less folded compared to CFTR associated chaperones. Moreover like IDPs, calumenin and other CREC proteins were found to be less hydrophobic and aggregation prone. Phylogenetic analysis revealed a close link between calumenin and other CREC proteins indicating how evolution might have shaped their similar biophysical properties. Experimentally, calumenin was observed to significantly reduce F508del-CFTR aggregation in a manner similar to AavLEA1, a well-characterized IDP. Fluorescence microscopy based imaging analysis also revealed altered trafficking of calumenin in bronchial cells expressing F508del-CFTR, indicating its direct role in the pathophysiology of CF. In conclusion, calumenin is characterized as a charged protein exhibiting close similarity with IDPs and is hypothesized to regulate F508del-CFTR folding by electrostatic effects. This work provides useful insights for designing optimized synthetic structural correctors of CFTR mutant proteins in the future. PMID:25120007

  1. Multimodal Assessment of Protein Functional Deficiency Supports Pathogenicity of BRCA1 p.V1688del

    PubMed Central

    De Nicolo, Arcangela; Parisini, Emilio; Zhong, Quan; Palma, Maurizia Dalla; Stoeckert, Kathryn A.; Domchek, Susan M.; Nathanson, Katherine L.; Caligo, Maria A.; Vidal, Marc; Cusick, Michael E.; Garber, Judy E.

    2009-01-01

    Unequivocal discrimination between neutral variants and deleterious mutations is crucial for appropriate counseling of individuals with a BRCA1 or BRCA2 sequence change. An increasing number of variants of uncertain significance (VUSs) are being identified, whose unclassified biological effect poses clinical concerns. A multifactorial likelihood-based approach recently suggested disease causality for BRCA1 p.V1688del, a VUS recurrent in Italian breast/ovarian cancer families. Whether and how this single amino acid deletion in the BRCA1 BRCT domain affects the function of the mutant protein (ΔValBRCA1) has not been elucidated. We undertook comprehensive functional characterization of ΔValBRCA1, comprising comparative structural modeling, analysis of protein stability and associations, and analysis of DNA repair function. Our model predicted BRCT domain destabilization and folding disruption caused by BRCA1 p.V1688del. Consistently, the recombinant ΔValBRCA1 was less stable than wtBRCA1 and, unlike the latter, failed to associate with BRIP1, CtIP, and Rap80, and to re-localize to sites of DNA damage. Yeast two-hybrid analysis revealed a compromised interaction with FHL2 and with KPNA2, which is likely responsible for improper subcellular localization of ΔValBRCA1. In addition, we found four new breast/ovarian cancer families of Italian ancestry who carried this sequence alteration. These results provide the first evidence of the effect of BRCA1 p.V1688del on protein stability and function, supporting the view that it is a deleterious mutation. Multimodal analyses like ours could advance understanding of tumor suppression by BRCA1, and ultimately contribute to developing efficient strategies for screening and characterization of VUSs. PMID:19706752

  2. Petroleum geology of the deltaic sequence, Rio Del Rey basin, offshore Cameroon

    SciTech Connect

    Coughlin, R.M.; Bement, W.O.; Maloney, W.V. )

    1993-09-01

    The Rio Del Rey Basin of offshore Cameroon comprises the easternmost portion of the Niger delta complex. In the delta flank setting, the overall package is thinner and stratigraphic correlation simpler than in the depocenter to the west so the distribution of reservoirs and seals is well defined. Productive reservoirs are shallow, typically less than 2000 m, and mostly hydropressured to slightly overpressured. Reservoir properties generally are excellent and seismic bright-spot technology has played a major role in exploration and development efforts. Rio Del Rey is characterized by three main structural styles from north to south: (1) detachment-based growth faults, (2) highly faulted, mobile shale cored domes and ridges, and (3) toe thrusts and folds. Virtually all individual traps are fault dependent and lack of sufficient internal seals in sand-rich wave-dominated deltaic sequences can limit the objective window. Most fields are downthrown fault traps in which hanging-wall reservoirs are effectively sealed against older prodelta and marine shales. All large oil fields are located in the dome and ridge province and are potential analogs for similar structures now being explored on the upper slope off Nigeria. The best quality source rocks identified thus far are Paleocene to Eocene marine shales. Oil vs. gas distribution in the basin is a function of variations in the kerogen type, organic richness, and maturity of these source rocks. A significant quantity of the gas found to date in Rio Del Rey is biogenic in origin. The Cameroon charge model may aid in better understanding hydrocarbon distribution on offshore Nigeria, where the Miocene deltaic section is much thicker and potential Paleocene-Eocene source rocks likely are unpenetrated. The shallow deltaic play in Cameroon is relatively mature. Future exploration likely will focus on identifying deeper, nonbright-spot-supported opportunities including potential stratigraphic traps.

  3. Online monitor detector for the protontherapy beam at the INFN Laboratori Nazionali del Sud-Catania

    NASA Astrophysics Data System (ADS)

    Givehchi, N.; Marchetto, F.; Boriano, A.; Attili, A.; Bourhaleb, F.; Cirio, R.; Cirrone, G. A. P.; Cuttone, G.; Di Rosa, F.; Donetti, M.; Garella, M. A.; Giordanengo, S.; Iliescu, S.; La Rosa, A.; Lojacono, P. A.; Nicotra, P.; Peroni, C.; Pecka, A.; Pitta, G.; Raffaele, L.; Russo, G.; Sabini, M. G.; Valastro, L. M.

    2007-03-01

    A detector to monitor online the protontherapy beam at the Istituto Nazionale di Fisica Nucleare (INFN), Laboratori Nazionali del Sud (LNS) has been built and characterized. The detector is made of two ionization chambers: each chamber has the anode splitted into 256 0.5 mm strips with vertical and horizontal orientation. The chambers are part of the beam line: signals can be processed online at a speed up to 100 Hz and results are promptly available. Thus the beam geometry can be controlled continuously during patient treatment, and in case of deviation from the required conditions, the treatment can be directly concluded.

  4. Stratigraphic context and direct dating of the Neandertal mandible from Cova del Gegant (Sitges, Barcelona).

    PubMed

    Daura, J; Sanz, M; Pike, A W G; Subir, M E; Forns, J J; Fullola, J M; Juli, R; Zilho, J

    2010-07-01

    Stratigraphic study of the Cova del Gegant's sedimentary fill revealed different cycles of accumulation of typical interior cave and delta facies. A precise chronology for these deposits, the faunal remains and stone tools contained therein was obtained by radiocarbon, U-Th and OSL. Our results indicate that the Upper Pleistocene archaeological sequence dates between 49.3 +/- 1.8 ka BP, the U-Th age of the overlying flowstone, and 60.0 +/- 3.9 ka BP, the OSL age of the basal deposits. We have also directly dated the site's Neandertal mandible to 52.3 +/- 2.3 ka by U-Th. PMID:20570316

  5. Identifying bipolar knapping in the Mesolithic site of Font del Ros (northeast Iberia).

    PubMed

    Roda Gilabert, Xavier; Mora, Rafael; Martnez-Moreno, Jorge

    2015-11-19

    Despite recent advances in the identification of bipolar knapping, its role in many sites is not well known. We propose to assess the significance of this technique in the context of changes that occur in the Mesolithic. A lithic assemblage was recovered from unit SG at Font del Ros (Catalunya, Spain) in which pitted stones, cores and products arising from bipolar reduction (flakes, fragments and splintered pieces) were identified. This study indicates that the bipolar technique is fundamental in the settlement. These results are key to defining the organization of Holocene hunter-gatherer subsistence in northeast Iberia. PMID:26483532

  6. [Nematodes parasitizing fishes from Termas de Rio Hondo Pond, Santiago del Estero, Argentina].

    PubMed

    Ramallo, G

    1999-01-01

    Cucullanus pinnai and Spinitectus jamundensis are cited parasitizing three freshwater fish species: Pimelodus albicans, Leporinus obtusidens and Prochilodus lineatus, captured in Termas de Ro Hondo pond, province of Santiago del Estero, Argentina, in 1992 and 1995. The three fish species were parasited by S. jamundensis, but C. pinnai was found only in P. albicans and L. obtusidens. Morphologic and morphometric parasitological studies were carried out. The specimens described were drawned. In addition, the following parasitic infection indexes were calculated: prevalence, mean and maximum intensity. The two nematode species identified are described for the first time in Argentina and increased the host range cited for the parasite species studied in this work. PMID:10488583

  7. Investigacin del USGS sobre el ecosistema de arrecifes de coral en el Atlntico

    USGS Publications Warehouse

    Kuffner, Ilsa B.; Yates, Kimberly K.; Zawada, David G.; Richey, Julie N.; Kellogg, Christina A.; Toth, Lauren T.; Torres-Garcia, Legna M.

    2015-01-01

    Los arrecifes de coral son estructuras slidas, biomineralizadas que protegen comunidades costeras actuando como barreras protectoras de peligros tales como los huracanes y los tsunamis. Estos proveen arena a las playas a travs de procesos naturales de erosin, fomentan la industria del turismo, las actividades recreacionales y proveen hbitats pesqueros esenciales. La conti-nua degradacin mundial de ecosistemas de arrecifes de coral est bien documentada. Existe la necesidad de enfoque y organizacin de la ciencia para entender los procesos complejos fsicos y biolgicos e interacciones que estn afectando el estado de los arrecifes coralinos y su capacidad para responder a un entorno cambiante.

  8. [Hierba del clavo (Geum chiloense) modifying cyclosporine levels: potential risk for transplanted people].

    PubMed

    Duclos, J; Goecke, H

    2001-07-01

    We report a 54 years old male that received a renal allograft without complications. One year after the transplantation, she was receiving cyclosporine doses of 2 to 3 mg/kg and maintained serum levels of 60 to 90 mg/dl. An abrupt increase in cyclosporine serum levels to 469 and 600 mg/dl was noted after 15 months of transplantation. After a careful interrogation the patient admitted the use of Geum chiloense ("hierba del clavo"). Discontinuing this herbal remedy, cyclosporine levels decreased to 55 mg/dl, despite the maintenance of the same cyclosporine dose. The potential side effects of herbal remedies must be borne in mind. PMID:11552449

  9. Eruption of the nevado del ruiz volcano, Colombia, on 13 november 1985: tephra fall and lahars.

    PubMed

    Naranjo, J L; Sigurdsson, H; Carey, S N; Fritz, W

    1986-08-29

    A small Plinian eruption of the Nevado del Ruiz volcano in Colombia ejected 3.5 x 10(10) kilograms of mixed dacite and andesite tephra on 13 November 1985, with a maximum column height of 31 kilometers above sea level. Small pyroclastic flows and surges, generated during the initial stage of the eruption, caused surface melting of approximately 10% of the volcano's ice cap, leading to meltwater floods. The erosive floods incorporated soils and loose sediments from the volcano's flanks and developed into lahars, which claimed at least 25,000 lives. PMID:17732038

  10. Early neogene history of the central American arc from Bocas del Toro, western Panama

    USGS Publications Warehouse

    Coates, A.G.; Aubry, M.-P.; Berggren, W.A.; Collins, Luke S.; Kunk, M.

    2003-01-01

    A newly discovered sequence of lower to middle Miocene rocks from the eastern Bocas del Toro archipelago, western Panama, reveals the timing and environment of the earliest stages in the rise of the Isthmus of Panama in this region. Two new formations, the Punta Alegre Formation (lower Miocene, Aquitanian to Burdigalian) and the Valiente Formation (middle Miocene, Langhian to Serravallian), are here named and formally described. The Punta Alegre Formation contains a diagnostic microfauna of benthic and planktic foraminifera and calcareous nannofossils that indicate deposition in a 2000-m-deep pre-isthmian neotropical ocean from as old as 21.5-18.3 Ma. Its lithology varies from silty mudstone to muddy foraminiferal ooze with rare thin microturbidite layers near the top. The Valiente Formation, which ranges in age from 16.4 to ca. 12.0 Ma, lies with slight angular unconformity on the Punta Alegre Formation and consists of five lithofacies: (1) columnar basalt and flow breccia, (2) pyroclastic deposits, (3) coarse-grained volcaniclastic deposits, (4) coral-reef limestone with diverse large coral colonies, and (5) marine debris-flow deposits and microturbidities. These lithofacies are interpreted to indicate that after ca. 16 Ma a volcanic arc developed in the region of Bocas del Toro and that by ca. 12 Ma an extensively emergent archipelago of volcanic islands had formed. 39Ar/40Ar dating of basalt flows associated with the fossiliferous sedimentary rocks in the upper part of the Valiente Formation strongly confirms the ages derived from planktic foraminifera and nannofossils. Paleobathymetric analysis of the two new formations in the Valiente Peninsula and Popa Island, in the Bocas del Toro archipelago, shows a general shallowing from lower- through upper-bathyal to upper-neritic and emergent laharic and fluviatile deposits from ca. 19 to 12 Ma. The overlying nonconformable Bocas del Toro Group contains a lower transgressive sequence ranging from basal nearshore sandstone to upper-bathyal mudstone (ca. 8.1-5.3. Ma) and an upper regressive sequence (5.3-3.5 Ma). A similar paleobathymetric pattern is observed from the Gatun to Chagres Formations (12-6 Ma) in the Panama Canal Basin area and in the Uscari, Rio Banana, Quebrada Chocolate, and Moin Formations (8-1.7 Ma) in the southern Limo??n Basin of Costa Rica.

  11. Uranium series dating of human skeletal remains from the Del Mar and Sunnyvale sites, California

    SciTech Connect

    Bischoff, J.L.; Rosenbauer, R.J.

    1981-08-28

    Uranium series analyses of human bone samples from the Del Mar and Sunnyvale sites indicate ages of 11,000 and 8300 years, respectively. The dates are supported by internal concordancy between thorium-230 and protactinium-231 decay systems. These ages are significantly younger than the estimates of 48,000 and 70,000 years based on amino acid racemization, and indicate that the individuals could derive from the population waves that came across the Bering Strait during the last sea-level low.

  12. Uranium series dating of human skeletal remains from the del mar and sunnyvale sites, california.

    PubMed

    Bischoff, J L; Rosenbauer, R J

    1981-08-28

    Uranium series analyses of human bone samples from the Del Mar and Sunnyvale sites indicate ages of 11,000 and 8,300 years, respectively. The dates are supported by internal concordancy between thorium-230 and protactinium-231 decay systems. These ages are significantly younger than the estimates of 48,000 and 70,000 years based on amino acid racemization, and indicate that the individuals could derive from the population waves that came across the Bering Strait during the last sea-level low. PMID:17789030

  13. 2.3-11.6 Micron Measurements of Nova Del 2013 Consistent with Presence of Dust

    NASA Astrophysics Data System (ADS)

    Cass, A. C.; Carlon, R. L.; Corgan, D. T.; Dykhoff, D. A.; Gehrz, R. D.; Shenoy, D. P.

    2013-11-01

    Our 2.3-11.6 micron measurements of Nova Del 2013 on 24.04 November 2013 UT using an As:Si bolometer mounted on the 0.76-m infrared telescope of the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA) yielded the following magnitudes: K = +5.24 +/- 0.15, L = +3.25 +/- 0.38, M = +2.00 +/- 0.26, [10.3] = -0.17 +/- 0.38, and [11.6] = -0.19 +/- 0.21. An additional measurement on 19.08 November 2013 yielded [10.3] = -0.10 +/- 0.36.

  14. L-Band Measurement of Nova Del 2013 Consistent with Presence of Dust

    NASA Astrophysics Data System (ADS)

    Cass, A. C.; Carlon, R. L.; Corgan, D. T.; Dykhoff, D. A.; Gehrz, R. D.; Shenoy, D. P.

    2013-09-01

    An L-band measurement of Nova Del 2013 on 29.17 September 2013 UT using an As:Si bolometer mounted on the 0.76-m infrared telescope of the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA) yielded a magnitude of L = +2.6 +/- 0.3. Vega (alpha Lyrae) was used as the standard star. This flux level is consistent with a small amount of dust having formed recently as reported in ATEL 5431.

  15. Evolution of the etna volcano: Information from the southern wall of the Valle Del Bove Caldera

    NASA Astrophysics Data System (ADS)

    McGuire, W. J.

    1982-08-01

    The greater part of Etna can be regarded as a complex strato-shield volcano constructed from the overlapping products of several centres of trachy-basaltic activity. The Valle del Bove is a horse-shoe-shaped caldera, 8 km long and 5 km wide, cut into the eastern flanks of Etna. The caldera is one of the few areas on the volcano where historic eruptions have not obscured the products of pre-historic centres of activity and these are well exposed in the cliff walls surrounding the caldera. Examination of these older volcanics provides important information on the eruptive style and internal plumbing of the Etna volcano during pre-historic times, and suggests that both were significantly different from the present day. Much of the southern wall of the Valle del Bove represents a surviving portion of the Trifoglietto II volcano, the largest pre-historic centre of activity. A stratigraphy is constructed for the southern wall, the Trifoglietto II lavas and pyroclastics rest unconformably upon the eroded remnants of an older centre, and are themselves overlain by the products of younger centres. All the lavas exposed in the southern wall are of alkalic affinity and comprise a trachybasaltic suite ranging from hawaiite to benmoreite. Variation in the chemistry of the lavas can be explained by their differentiation at high levels in the crust from a more basic magma of alkali olivine-basalt/hawaiite composition. An anomalous trend in the TiO 2 content of the Trifoglietto II lavas may be explained by the fractionation of kaersutite (Ti-rich amphibole). A study has been made of the numerous dykes exposed in the walls of the Valle del Bove, the alignments of which parallel trends which are important on Etna at the present time. It is proposed that the initial opening of the Valle del Bove occurred sometime between 20,000 and 10,000 y. B.P., as a result of a phreatic or phreato-magmatic explosion near the base of the eastern flank of Trifoglietto II. This is visualised as triggering a slope failure and resulting in the destruction of much of the centre by a catastrophic landslide. This mechanism has much in common with the explosive eruptions which produced both the Bandai-san (Japan) caldera in 1888, and the Mount St Helens caldera in May, 1980.

  16. Frequency of CHEK2*1100delC in New York breast cancer cases and controls

    PubMed Central

    Offit, Kenneth; Pierce, Heather; Kirchhoff, Tomas; Kolachana, Prema; Rapaport, Beth; Gregersen, Peter; Johnson, Steven; Yossepowitch, Orit; Huang, Helen; Satagopan, Jaya; Robson, Mark; Scheuer, Lauren; Nafa, Khedoudja; Ellis, Nathan

    2003-01-01

    Background The 1100delC CHEK2 allele has been associated with a 1.4–4.7 fold increased risk for breast cancer in women carrying this mutation. While the frequency of 1100delC was 1.1–1.4% in healthy Finnish controls, the frequency of this allele in a North American control population and in North American breast cancer kindreds remains unclear. Methods We genotyped 1665 healthy New York volunteers and 300 cases of breast cancer for the CHEK2*1100delC. Results The overall frequency of the 1100delC was 3/300 (1.0%) among all cases with either a family history of breast cancer (n = 192) or a personal history of breast cancer (n = 108, of which 46 were bilateral, 46 unilateral, and 16 were male breast cancer cases), compared to a frequency of 5/1665 (0.3%) in healthy controls (p = 0.1). There was no difference in allele frequency among Ashkenazi and non-Ashkenazi controls. Conclusion The relatively low breast cancer penetrance of this allele, along with the low population frequency, will limit the clinical applicability of germline testing for CHEK2*1100delC in North American kindreds. PMID:12529183

  17. Astronoma para ciegos y amblopes. Proyecto de construccin de un planetario especial en la ciudad de Mar del Plata

    NASA Astrophysics Data System (ADS)

    Musso, S.

    ?`Qu es la Astronoma para Ciegos?. El trabajo es una adaptacin en base a la escala de magnitudes de Hipparco que cambia el concepto de luz por una adaptacin sonora, donde las estrellas de magnitud 6 se escuchan en 10 dB, ms o menos lo que consideramos el umbral de la audicin humana. Quienes no escuchan muy bien no pueden escuchar las magnitudes 6, de la misma manera que muchos de nosotros, que no poseemos una visin perfecta, no podemos observar esas mismas estrellas en el cielo (ms all de la polucin). A los astros de magnitud 5 vamos a relacionarlos a un sonido en 20 dB. Y as sucesivamente. Tambin los colores estarn representados en una convencin de graves a agudos y lo mismo algunas caractersticas del cielo. Por ejemplo, la Va Lctea se mostrar como un ``ruido", como bien nos lo hicieron ver nuestros futuros destinatarios. En Mar del Plata nos encontramos ya trabajando en un proyecto que tiene como objetivo final la construccin del Primer Planetario Acstico del Mundo, una herramienta para la enseanza de la astronoma, un espacio para la lucha contra la discriminacin del discapacitado y una posibilidad de ``ver el cielo de un modo diferente".

  18. Developing market class specific InDel markers from next generation sequence data in Phaseolus vulgaris L.

    PubMed Central

    Moghaddam, Samira Mafi; Song, Qijian; Mamidi, Sujan; Schmutz, Jeremy; Lee, Rian; Cregan, Perry; Osorno, Juan M.; McClean, Phillip E.

    2013-01-01

    Next generation sequence data provides valuable information and tools for genetic and genomic research and offers new insights useful for marker development. This data is useful for the design of accurate and user-friendly molecular tools. Common bean (Phaseolus vulgaris L.) is a diverse crop in which separate domestication events happened in each gene pool followed by race and market class diversification that has resulted in different morphological characteristics in each commercial market class. This has led to essentially independent breeding programs within each market class which in turn has resulted in limited within market class sequence variation. Sequence data from selected genotypes of five bean market classes (pinto, black, navy, and light and dark red kidney) were used to develop InDel-based markers specific to each market class. Design of the InDel markers was conducted through a combination of assembly, alignment and primer design software using 1.6 to 5.1 coverage of Illumina GAII sequence data for each of the selected genotypes. The procedure we developed for primer design is fast, accurate, less error prone, and higher throughput than when they are designed manually. All InDel markers are easy to run and score with no need for PCR optimization. A total of 2687 InDel markers distributed across the genome were developed. To highlight their usefulness, they were employed to construct a phylogenetic tree and a genetic map, showing that InDel markers are reliable, simple, and accurate. PMID:24860578

  19. Genetic Inhibition Of The Ubiquitin Ligase Rnf5 Attenuates Phenotypes Associated To F508del Cystic Fibrosis Mutation

    PubMed Central

    Tomati, Valeria; Sondo, Elvira; Armirotti, Andrea; Caci, Emanuela; Pesce, Emanuela; Marini, Monica; Gianotti, Ambra; Ju Jeon, Young; Cilli, Michele; Pistorio, Angela; Mastracci, Luca; Ravazzolo, Roberto; Scholte, Bob; Ronai, Ze’ev; Galietta, Luis J. V.; Pedemonte, Nicoletta

    2015-01-01

    Cystic fibrosis (CF) is caused by mutations in the CFTR chloride channel. Deletion of phenylalanine 508 (F508del), the most frequent CF mutation, impairs CFTR trafficking and gating. F508del-CFTR mistrafficking may be corrected by acting directly on mutant CFTR itself or by modulating expression/activity of CFTR-interacting proteins, that may thus represent potential drug targets. To evaluate possible candidates for F508del-CFTR rescue, we screened a siRNA library targeting known CFTR interactors. Our analysis identified RNF5 as a protein whose inhibition promoted significant F508del-CFTR rescue and displayed an additive effect with the investigational drug VX-809. Significantly, RNF5 loss in F508del-CFTR transgenic animals ameliorated intestinal malabsorption and concomitantly led to an increase in CFTR activity in intestinal epithelial cells. In addition, we found that RNF5 is differentially expressed in human bronchial epithelia from CF vs. control patients. Our results identify RNF5 as a target for therapeutic modalities to antagonize mutant CFTR proteins. PMID:26183966

  20. miR-16 rescues F508del-CFTR function in native cystic fibrosis epithelial cells.

    PubMed

    Kumar, P; Bhattacharyya, S; Peters, K W; Glover, M L; Sen, A; Cox, R T; Kundu, S; Caohuy, H; Frizzell, R A; Pollard, H B; Biswas, R

    2015-11-01

    Cystic fibrosis (CF) is due to mutations in the CFTR gene, which prevents correct folding, trafficking and function of the mutant cystic fibrosis transmembrane conductance regulator (CFTR) protein. The dysfunctional effect of CFTR mutations, principally the F508del-CFTR mutant, is further manifested by hypersecretion of the pro-inflammatory chemokine interleukin-8 into the airway lumen, which further contributes to morbidity and mortality. We have hypothesized that microRNA (miR)-based therapeutics could rescue the dysfunctional consequences of mutant CFTR. Here we report that a miR-16 mimic can effectively rescue F508del-CFTR protein function in airway cell lines and primary cultures, of differentiated human bronchial epithelia from F508del homozygotes, which express mutant CFTR endogenously. We also identify two other miRs, miR-1 and miR-302a, which are also active. Although miR-16 is expressed at basal comparable levels in CF and control cells, miR-1 and miR-302a are undetectable. When miR mimics are expressed in CF lung or pancreatic cells, the expression of the F508del-CFTR protein is significantly increased. Importantly, miR-16 promotes functional rescue of the cyclic AMP-activated apical F508del-CFTR chloride channel in primary lung epithelial cells from CF patients. We interpret these findings to suggest that these miRs may constitute novel targets for CF therapy. PMID:26133785

  1. Developing market class specific InDel markers from next generation sequence data in Phaseolus vulgaris L.

    PubMed

    Moghaddam, Samira Mafi; Song, Qijian; Mamidi, Sujan; Schmutz, Jeremy; Lee, Rian; Cregan, Perry; Osorno, Juan M; McClean, Phillip E

    2014-01-01

    Next generation sequence data provides valuable information and tools for genetic and genomic research and offers new insights useful for marker development. This data is useful for the design of accurate and user-friendly molecular tools. Common bean (Phaseolus vulgaris L.) is a diverse crop in which separate domestication events happened in each gene pool followed by race and market class diversification that has resulted in different morphological characteristics in each commercial market class. This has led to essentially independent breeding programs within each market class which in turn has resulted in limited within market class sequence variation. Sequence data from selected genotypes of five bean market classes (pinto, black, navy, and light and dark red kidney) were used to develop InDel-based markers specific to each market class. Design of the InDel markers was conducted through a combination of assembly, alignment and primer design software using 1.6 to 5.1 coverage of Illumina GAII sequence data for each of the selected genotypes. The procedure we developed for primer design is fast, accurate, less error prone, and higher throughput than when they are designed manually. All InDel markers are easy to run and score with no need for PCR optimization. A total of 2687 InDel markers distributed across the genome were developed. To highlight their usefulness, they were employed to construct a phylogenetic tree and a genetic map, showing that InDel markers are reliable, simple, and accurate. PMID:24860578

  2. Lumacaftor alone and combined with ivacaftor: preclinical and clinical trial experience of F508del CFTR correction.

    PubMed

    Brewington, John J; McPhail, Gary L; Clancy, John P

    2016-01-01

    Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator protein (CFTR), leading to significant morbidity and mortality. CFTR is a chloride and bicarbonate channel at the epithelial cell membrane. The most common CFTR mutation is F508del, resulting in minimal CFTR at the plasma membrane. Current disease management is supportive, whereas an ultimate goal is to develop therapies to restore CFTR activity. We summarize experience with lumacaftor, a small molecule that increases F508del-CFTR levels at the plasma membrane. Lumacaftor in combination with ivacaftor, a modulator of CFTR gating defects, improves clinical outcome measures in patients homozygous for the F508del mutation. Lumacaftor represents a significant advancement in the treatment of biochemical abnormalities in CF. Further development of CFTR modulators will improve upon current therapies, although it remains unclear whether this approach will provide therapies for all CFTR mutations. PMID:26581802

  3. Loss of helminth species diversity in the large hairy armadillo Chaetophractus villosus on the Tierra del Fuego Island, Argentina.

    PubMed

    Ezquiaga, M C; Abba, A M; Navone, G T

    2016-03-01

    The aim of this work is to compare the taxonomic diversity of parasite species of the large hairy armadillo Chaetophractus villosus in its native range and in another recently introduced population (Tierra del Fuego island), and to evaluate whether the isolation of the latter determines a decrease in its parasitic diversity. Forty specimens from Buenos Aires and Tierra del Fuego Provinces were collected and examined for helminths. Eleven parasite species were found in the native population, and only one species was present in Tierra del Fuego (Trichohelix tuberculata). This may be explained because isolation and climatic conditions prevent encounters between potential host species and infective forms of parasites. Further sampling will be needed throughout the entire Patagonia steppe to confirm how the characteristic parasitic fauna of C. villosus behaves across the armadillo's southern distribution. PMID:25673233

  4. Role of casein kinase 1A1 in the biology and targeted therapy of del(5q) MDS.

    PubMed

    Schneider, Rebekka K; Adem, Vera; Heckl, Dirk; Jrs, Marcus; Mallo, Mar; Lord, Allegra M; Chu, Lisa P; McConkey, Marie E; Kramann, Rafael; Mullally, Ann; Bejar, Rafael; Sol, Francesc; Ebert, Benjamin L

    2014-10-13

    The casein kinase 1A1 gene (CSNK1A1) is a putative tumor suppressor gene located in the common deleted region for del(5q) myelodysplastic syndrome (MDS). We generated a murine model with conditional inactivation of Csnk1a1 and found that Csnk1a1 haploinsufficiency induces hematopoietic stem cell expansion and a competitive repopulation advantage, whereas homozygous deletion induces hematopoietic stem cell failure. Based on this finding, we found that heterozygous inactivation of Csnk1a1 sensitizes cells to a CSNK1 inhibitor relative to cells with two intact alleles. In addition, we identified recurrent somatic mutations in CSNK1A1 on the nondeleted allele of patients with del(5q) MDS. These studies demonstrate that CSNK1A1 plays a central role in the biology of del(5q) MDS and is a promising therapeutic target. PMID:25242043

  5. La meridiana di Egnazio Danti nella Torre dei Venti in Vaticano: un'icona della riforma Gregoriana del calendario

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2014-05-01

    La Torre dei Venti domina l’angolo Sud Ovest del cortile della Pigna (nell'area dei Musei Vaticani), ed è inclusa negli ambienti dell'Archivio Segreto Vaticano. Non è aperta al pubblico, ma è universalmente nota per la fama che da oltre quattrocento anni la circonda, legata alle vicende della riforma Gregoriana del calendario. La meridiana tracciata da padre Egnazio Danti (1536-1586) nella torre dei Venti, fu visitata anche da Gregorio XIII, probabilmente il 21 marzo 1581 come suppone il padre Stein, per convincersi dell'anticipo ormai arrivato a dieci giorni dell'equinozio di primavera sulla data che il concilio di Nicea aveva fissato al 21 marzo per il computo pasquale. La ricognizione astrometrica del febbraio-marzo 2009 fatta dall'autore viene qui presentata.

  6. Role of casein kinase 1A1 in the biology and targeted therapy of del(5q) MDS

    PubMed Central

    Schneider, Rebekka K.; Adem, Vera; Heckl, Dirk; Jrs, Marcus; Mallo, Mar; Lord, Allegra M.; Chu, Lisa P.; McConkey, Marie E.; Kramann, Rafael; Mullally, Ann; Bejar, Rafael; Sol, Francesc; Ebert, Benjamin L.

    2014-01-01

    Summary The Casein kinase 1A1 gene (CSNK1A1) is a putative tumor suppressor gene located in the common deleted region for del(5q) myelodysplastic syndrome (MDS). We generated a murine model with conditional inactivation of Csnk1a1 and found that Csnk1a1 haploinsufficiency induces hematopoietic stem cell expansion and a competitive repopulation advantage whereas homozygous deletion induces hematopoietic stem cell failure. Based on this finding, we found that heterozygous inactivation of Csnk1a1 sensitizes cells to a CSNK1 inhibitor relative to cells with two intact alleles. In addition, we identified recurrent somatic mutations in CSNK1A1 on the non-deleted allele of patients with del(5q) MDS. These studies demonstrate that CSNK1A1 plays a central role in the biology of del(5q) MDS and is a promising therapeutic target. PMID:25242043

  7. Expression of a cyanobacterial {del}{sup 6}-desaturase gene results in {gamma}-linolenic acid production in transgenic plants

    SciTech Connect

    Reddy, A.S.; Thomas, T.L.

    1996-05-01

    Gamma-linolenic acid (GLA), a nutritionally important fatty acid in human and animal diets, is not produced in oil seed crops. Many oil seed plants, however, produce significant quantities of linoleic acid, a fatty acid that could be converted to GLA by the enzyme {del}{sup 6}-desaturase if it were present. As a first step to producing GLA in oil seed crops, we have cloned a cyanobacterial {del}{sup 6}-desaturase gene. Expression of this gene in transgenic tobacco resulted in GLA accumulation. Octadecatetraenoic acid, a highly unsaturated, industrially important fatty acid, was also found in transgenic tobacco plants expressing the cyanobacterial {del}{sup 6}-desaturase. This is the first example of engineering the production of `novel` polyunsaturated fatty acids in transgenic plants. 28 refs., 4 figs., 1 tab.

  8. VizieR Online Data Catalog: 1 Del spectroscopy (Kubat+, 2016)

    NASA Astrophysics Data System (ADS)

    Kubat, J.; Kubatova, B.; Dolezalova, B.; Iliev, L.; Slechta, M.

    2016-02-01

    Visual spectra were observed with the Perek 2-m telescope at the Ondrejov Observatory (Czech Republic) with the slit spectrograph in coude focus. We obtained medium-resolution spectra, (R~13000) with the 700-mm spectrograph focus, in which the SITe CCD 2030x800 chip of 15um pixels was used. Our spectra of 1 Del were taken on 16 September 2007. The data were reduced (bias subtracted, flat-fielded, and wavelength calibrated) using the Image Reduction and Analysis Facility (IRAF1). The spectra were normalized to continuum with a straight line to fit the far wings of the Hα line. SINFONI observations were obtained in service mode at the VLT-UT4 (Yepun) telescope at ESO under Programme-ID 093.D-0172(A) during two nights (6 and 10 June 2014). Spectroscopy of 1 Del was carried out with the SINFONI, the AO-assisted integral field spectrograph in all available near-infrared bands (J, H, and K). (2 data files).

  9. Mesoscopic faults in the granite of Isola del Giglio (Tuscan Archipelago)

    NASA Astrophysics Data System (ADS)

    Passerini, P.; Marcucci, M.

    1992-06-01

    Faults at the mesoscopic scale in the granite at Isola del Giglio are examined by considering the strike, dip, pitch of the striae and minerals on the fault planes. The pattern of faulting is areally variable. Dykes and veins are often parallel to faults, suggesting a genetic connection. A major system of faults, mainly reverse and steeply dipping, strikes north-northwest and is characterized by the frequent occurrence of crushed tourmaline, these faults are often developed along pegmatite veinlets or dykes, and were probably produced by a combination of hydraulic and shear fracturing in the final stage of cooling of the granite. Another prominent phase of faulting is testified to by ENE-striking fault planes, strike-slip to oblique-slip, devoid of tourmaline and mostly coated with chlorite. p ]The prevalence of reverse and strike-slip faults over normal faults contradicts the traditional views about dominantly extensional tectonics in this area during the Pliocene and Quaternary. Extensional deformation at Isola del Giglio ended with the granite intrusion; crustal melting and extension ended at about 5 Ma, after which subvertical and strike-slip movements became dominant.

  10. Large residuals on geoidal heights determined on the Fagnano Lake, Tierra del Fuego-Argentina

    NASA Astrophysics Data System (ADS)

    Gomez, M. E.; Del Cogliano, D.; Perdomo, R.

    2013-05-01

    A new geoid model was developed in Tierra del Fuego and it was evaluated in the area of Fagnano Lake. The model was developed by means of the Equivalent Source Technique combining gravity data, levelling information measured on the province and observations of a GPS buoy on the Fagnano Lake. Those GPS buoy measurements provide information of the mean lake level surface (Del Cogliano et al., 2007). A cross validation process was realized in order to evaluate the model on the lake. What allowed determining a 6 cm geoid in the area of Fagnano Lake. Also, an evaluation of the EGM2008 (Pavlis et al., 2008) was made on the lake. Its behaviour was compared to that observed on the levelling lines. Differences of several decimetres were found when EGM2008 undulations were compared to observed geoid undulations in the lake area. In the regions where EGM2008 has included real gravimetric observations, differences between model and observations were only of a few centimetres. Such model has the particularity that includes fill-in gravity in that region. The above mentioned evaluation derived in an analysis of the effect that not representative gravity information could have on the estimation of geoid undulations in high mountainous regions. We found that this effect could be significant if there is no real information in the computing area (Gomez et al, 2012).

  11. Structural evolution of the Sarand del Y Shear Zone, Uruguay: kinematics, deformation conditions and tectonic significance

    NASA Astrophysics Data System (ADS)

    Oriolo, S.; Oyhantabal, P.; Heidelbach, F.; Wemmer, K.; Siegesmund, S.

    2015-10-01

    The Sarand del Y Shear Zone is a crustal-scale shear zone that separates the Piedra Alta Terrane from the Nico Prez Terrane and the Dom Feliciano Belt in southern Uruguay. It represents the eastern margin of the Ro de la Plata Craton and, consequently, one of the main structural features of the Precambrian basement of Western Gondwana. This shear zone first underwent dextral shearing under upper to middle amphibolite facies conditions, giving rise to the reactivation of pre-existing crustal fabrics in the easternmost Piedra Alta Terrane. Afterwards, pure-shear-dominated sinistral shearing with contemporaneous magmatism took place under lower amphibolite to upper greenschist facies conditions. The mylonites resulting from this event were then locally reactivated by a cataclastic deformation. This evolution points to strain localization under progressively retrograde conditions with time, indicating that the Sarand del Y Shear Zone represents an example of a thinning shear zone related to the collisional to post-collisional evolution of the Dom Feliciano Belt that occurred between the Meso- to Neoproterozoic (>600 Ma) and late Ediacaran-lower Cambrian times.

  12. Phenomena that influence high ozone concentrations in the Paso del Norte area

    SciTech Connect

    MacDonald, C.P.; Roberts, P.T.; Main, H.H.; Dye, T.S.; Yarbrough, J.

    1998-12-31

    This paper summarizes the results of analyses of meteorological and air quality data for the 1996 Paso del Norte Ozone Study. The purpose of these data analyses was to develop an understanding of the significant chemical and physical processes which lead to high concentrations of ozone in the Paso del Norte study region which includes El Paso, TX; Sunland Park, NM; and Juarez, Mexico. This analysis has shown that the high ozone concentrations are a result of slow convective boundary layer growth, reduced convective boundary layer depth, light surface winds, and high morning surface ozone precursor concentrations. Overlying meteorological conditions observed during high ozone episodes include an upstream aloft high pressure system, aloft warming, and weak surface pressure gradients. The analysis has also shown that aloft carryover of ozone and ozone precursors does not significantly contribute to the surface ozone concentration. Furthermore, hydrocarbon and NO{sub x} data, although spatially limited, show that the system is most often NO{sub x} rather than VOC limited.

  13. Light scattering from aerosol particles in the El Paso del Norte region / the effect of humidity

    NASA Astrophysics Data System (ADS)

    Medina Calderon, Richard

    Atmospheric aerosols play an important role in climate forcing, through scattering and absorption of the incoming solar radiation. The extinction of light by the presence of atmospheric aerosols was studied using two first-principle models, and corresponding computer codes. In the first model the extinction of light from irregularly shaped aerosol particles was analyzed. In the second model it was assumed that the irregularly shaped aerosol particles were covered by a film of water, and the hygroscopicity and the extinction of light by the aerosols was analyzed. These models were then applied to the Paso del Norte region and their light extinction results compared with a local extinctiometer. The inter-comparison of the models extinction results and the extinctiometer values were well correlated. It was observed that for high humidity days the model that used an aerosol particle covered with a water film correlated better with the experimental extinctiometer measurements. While these two models were validated in the Paso del Norte region, they are also applicable to any other region, under humid or dry atmospheric conditions.

  14. Paso del Norte pilot border study of ozone precursors and air toxics

    SciTech Connect

    Zielinska, B.; Sheetz, L.; Harshfield, G.

    1996-12-31

    A comprehensive monitoring program for ozone precursors and air toxics in the Paso del Norte border area is planned by the U.S. EPA for the Summer of 1996. A pilot study was carried out in October 1995 in the Paso del Norte area (El Paso, Texas, Ciudad Juarez, Chihuahua, and Sunland Park, New Mexico) to test the appropriateness of proposed sampling and analysis methods and to provide preliminary data to be used for planning the Summer 1996 study. Two monitoring sites were selected, one in Ciudad Juarez, and one in the El Paso area. Samples were collected every second day from October 21 to October 31, from 0300 to 0900 hr using stainless steel canisters (for VOC in the C{sub 2}-C{sub 12} range), Tenax-TA solid adsorbent cartridges (for C{sub 8}-C{sub 20} hydrocarbons). DNPH impregnated C{sub 18} Sep-Pack cartridges (for carbonyl compounds) and Teflon impregnated glass fiber filters followed by PUF/YAD/PUF {open_quotes}sandwich{close_quotes} cartridges (for SVOC). This paper discusses the data set obtained from the analyses of these samples. 6 refs., 7 figs.

  15. Cystic fibrosis patients from the Black Sea region: the 1677delTA mutation.

    PubMed

    Angelicheva, D; Boteva, K; Jordanova, A; Savov, A; Kufardjieva, A; Tolun, A; Telatar, M; Akarsuba?i, A; Kprba?i, F; Aydo?du, S

    1994-01-01

    A 2 bp deletion in exon 10 of the CFTR gene, 1677delTA, which is very rare among CF chromosomes worldwide, was found to be a relatively common cause of cystic fibrosis in countries located in the region of the Black Sea. The frequency of the mutation was compared among cystic fibrosis patients from several populations, namely Bulgarians, Turks, Greek-Cypriots, Georgians, and Russians. The deletion is most common among Georgian CF patients and gradually declines in frequency in neighbouring populations. It is invariably related to a common polymorphic haplotype which is rare among normal chromosomes in Bulgaria but was found to be common in Turkey. The geographic gradient in the frequency of the mutation, along with findings on polymorphic haplotype distribution, suggest that the mutation is relatively young in evolutionary terms and spread as the result of west and south-bound migrations originating from Georgia. The 1677delTA mutation is related to a severe clinical phenotype with a high early mortality rate among homozygotes and possibly to an increased risk of meconium ileus. PMID:8081388

  16. Receiver Function Analyses of Uturuncu Volcano, Bolivia and Lastarria/Cordon Del Azufre Volcanoes, Chile

    NASA Astrophysics Data System (ADS)

    Mcfarlin, H. L.; Christensen, D. H.; Thompson, G.; McNutt, S. R.; Ryan, J. C.; Ward, K. M.; Zandt, G.; West, M. E.

    2014-12-01

    Uturuncu Volcano and a zone between Lastarria and Cordon del Azufre Volcanoes (also calledLazufre), have seen much attention lately because of significant and rapid inflation of one to twocentimeters per year over large areas. Uturuncu is located near the Bolivian-Chilean border, andLazufre is located near the Chilean-Argentine border. The PLUTONS Project deployed 28broadband seismic stations around Uturuncu Volcano, from April 2009 to Octobor 2012, and alsodeployed 9 stations around Lastarria and Cordon del Azufre volcanoes, from November, 2011 toApril 2013. Teleseismic receiver functions were generated using the time-domain iterativedeconvolution algorithm of Ligorria and Ammon (1999) for each volcanic area. These receiverfunctions were used to better constrain the depths of magma bodies under Uturuncu and Lazufre,as well as the ultra low velocity layer within the Altiplano-Puna Magma Body (APMB). Thelow velocity zone under Uturuncu is shown to have a top around 10 km depth b.s.l and isgenerally around 20 km thick with regional variations. Tomographic inversion shows a well resolved,near vertical, high Vp/Vs anomaly directly beneath Uturuncu that correlates well with adisruption in the receiver function results; which is inferred to be a magmatic intrusion causing alocal thickening of the APMB. Preliminary results at Lazufre show the top of a low velocityzone around 5-10 km b.s.l with a thickness of 15-30 km.

  17. BRCA1 185delAG Mutation Enhances Interleukin-1? Expression in Ovarian Surface Epithelial Cells

    PubMed Central

    Woolery, Kamisha T.; Mohamed, Mai; Linger, Rebecca J.; Dobrinski, Kimberly P.; Roman, Jesse; Kruk, Patricia A.

    2015-01-01

    Familial history remains the strongest risk factor for developing ovarian cancer (OC) and is associated with germline BRCA1 mutations, such as the 185delAG founder mutation. We sought to determine whether normal human ovarian surface epithelial (OSE) cells expressing the BRCA1 185delAG mutant, BRAT, could promote an inflammatory phenotype by investigating its impact on expression of the proinflammatory cytokine, Interleukin-1? (IL-1?). Cultured OSE cells with and without BRAT were analyzed for differential target gene expression by real-time PCR, western blot, ELISA, luciferase reporter, and siRNA assays. We found that BRAT cells expressed increased cellular and secreted levels of active IL-1?. BRAT-expressing OSE cells exhibited 3-fold enhanced IL-1? mRNA expression, transcriptionally regulated, in part, through CREB sites within the (?1800) to (?900) region of its promoter. In addition to transcriptional regulation, BRAT-mediated IL-1? expression appears dualistic through enhanced inflammasome-mediated caspase-1 cleavage and activation of IL-1?. Further investigation is warranted to elucidate the molecular mechanism(s) of BRAT-mediated IL-1? expression since increased IL-1? expression may represent an early step contributing to OC. PMID:26357657

  18. DelPhi webserver: Comprehensive suite for electrostatic calculations of biological macromolecules and their complexes

    NASA Astrophysics Data System (ADS)

    Witham, Shawn; Boylen, Brett; Owesen, Barr; Rocchia, Walter; Alexov, Emil

    2011-03-01

    Electrostatic forces and energies are two of the major components that contribute to the stability, function and interaction of biological macromolecules. The calculations of the electrostatic potential distribution in such systems, which are comprised of irregularly shaped objects immersed in a water phase, is not a trivial task. In addition, an accurate model requires any missing hydrogen atoms of the corresponding structural files (Protein Data Bank, or, PDB files) to be generated in silico and, if necessary, missing atoms or residues to be predicted as well. Here we report a comprehensive suite, an academic DelPhi webserver, which allows the users to upload their structural file, calculate the components of the electrostatic energy, generate the corresponding potential (and/or concentration/dielectric constant) distribution map, and choose the appropriate force field. The webserver utilizes modern technology to take user input and construct an algorithm that suits the users specific needs. The webserver uses Clemson University's Palmetto Supercomputer Cluster to handle the DelPhi calculations, which can range anywhere from small and short computation times, to extensive and computationally demanding runtimes. The work was supported by a grant from NIGMS, NIH, grant number 1R01GM093937-01.

  19. Small molecule correctors of F508del-CFTR discovered by structure-based virtual screening

    PubMed Central

    Mense, Martin; Fischman, Sharon; Shitrit, Alina; Bihler, Hermann; Ben-Zeev, Efrat; Schutz, Nili; Pedemonte, Nicoletta; Thomas, Philip J.; Bridges, Robert J.; Wetmore, Diana R.; Marantz, Yael; Senderowitz, Hanoch

    2014-01-01

    Folding correctors of F508del-CFTR were discovered by in silico structure-based screening utilizing homology models of CFTR. The intracellular segment of CFTR was modeled and three cavities were identified at inter-domain interfaces: (1) Interface between the two Nucleotide Binding Domains (NBDs); (2) Interface between NBD1 and Intracellular Loop (ICL) 4, in the region of the F508 deletion; (3) multi-domain interface between NBD1:2:ICL1:2:4. We hypothesized that compounds binding at these interfaces may improve the stability of the protein, potentially affecting the folding yield or surface stability. In silico structure-based screening was performed at the putative binding-sites and a total of 496 candidate compounds from all three sites were tested in functional assays. A total of 15 compounds, representing diverse chemotypes, were identified as F508del folding correctors. This corresponds to a 3% hit rate, ∼tenfold higher than hit rates obtained in corresponding high-throughput screening campaigns. The same binding sites also yielded potentiators and, most notably, compounds with a dual corrector-potentiator activity (dual-acting). Compounds harboring both activity types may prove to be better leads for the development of CF therapeutics than either pure correctors or pure potentiators. To the best of our knowledge this is the first report of structure-based discovery of CFTR modulators. PMID:20976528

  20. La estructura dinmica del cinturn de Kuiper interior y la formacin de Urano y Neptuno

    NASA Astrophysics Data System (ADS)

    Melita, M. D.; Brunini, A.

    De acuerdo a los ms recientes estudios sobre la formacin de Urano y Neptuno, stos podran haber sufrido una importante migracin radial hacia el exterior, debido al intercambio de momento angular con los planetesimales presentes en esa regin durante las ltimas etapas de la formacin del Sistema Solar. Esta migracin radial habra tenido importantes consecuencias sobre la presente estructura dinmica del cinturn cometario de Kuiper. Tal como lo reflejan las observaciones, la mayora de los cuerpos observados en el cinturn de Kuiper se encuentran en resonancia de movimientos medios exteriores con Neptuno, hecho que tambin se refleja en la presente rbita de Plutn. En el presente trabajo se estudia tanto numrica como analticamente la influencia de la variacin de las masas de Urano y Neptuno durante el proceso de migracin sobre las probabilidades de captura en algunas de las resonancias exteriores de Neptuno. Se estudia tambin la influencia de encuentros gravitacionales mutuos sobre la estabilidad de las rbitas resonantes. Este estudio ofrece argumentos de plausibilidad para explicar por qu ninguno de los objetos descubiertos en el cinturn de Kuiper se encontrara en la resonancia 2:1.

  1. Implementation of tsunami disaster prevention measures in the municipality of San Rafael del Sur, Nicaragua

    NASA Astrophysics Data System (ADS)

    Strauch, W.; Talavera, E.; Acosta, N.; Sanchez, M.; Mejia, E.

    2007-05-01

    The Nicaraguan Pacific coast presents considerable tsunami risk. On September 1, 1992, a tsunami caused enormous damage in the infrastructure and killed more than 170 people. A pilot project was conducted between 2006 and 2007 in the municipality of San Rafel del Sur, area of Masachapa, The project included multiple topics of tsunami prevention measures and considering the direct participation of the local population, as: -General education on disaster prevention, participative events; -Investigation of awareness level and information needs for different population groups; -Specific educational measures in the schools; -Publication of brochures, calendars, news paper articles, radio programs, TV spots -Development of local tsunami hazard maps, 1:5,000 scale; (based on previous regional tsunami hazard mapping projects and local participation) -Development of a tsunami warning plan; -Improvements of the national tsunami warning system. -Installation of sirens for tsunami warning -Installation of tsunami signs, indicating hazardous areas, evacuation routes, safe places; -Realization of evacuation drills in schools. Based on the experiences gained in Masachapa it is planned to run similar projects in other areas along the Nicaraguan Pacific coast. In the project participated the local municipality and local stakeholders of San Rafael del Sur, Ministry of Education, National Police, Nicaraguan Red Cross, Ministry of Health, Ministry of Tourism, Nicaraguan Geosciences Institute (INETER), National System for Disaster Prevention (SINAPRED), Swiss Agency for Development and Cooperation (SDC). It was financed by SDC and INETER.

  2. Estudio del comportamiento tribologico y de las interacciones de superficie de nuevos nanofluidos ionicos

    NASA Astrophysics Data System (ADS)

    Espinosa Rodriguez, Tulia

    Ionic liquids are molten salts which are liquid at room temperature or at low temperatures and present a unique combination of properties. In the present work, we focus on their use as lubricants in complex tribological problems such as the lubrication of metals that slide against themselves, the development of water based lubricants and new self-lubricated surfaces. When it is difficult to reduce friction and wear by lubrication, as in the case of magnesium alloys, ionic liquids are studied as protective coatings precursors. Surface interactions and corrosion processes with protic and aprotic ionic liquids on copper and steel have been determined in order to develop new lubricants and lubricant additives. In the copper/copper contact, all ionic liquids present better tribological performance than the polyalphaolefin synthetic oil, except for the oleate derivative. New protic ionic liquids are not only exceptional lubricants of the steel/sapphire contact as neat lubricants, but when they are used as additives in water, the formation of a boundary layer after water evaporation occurs, thus reducing friction and wear. The formation of this boundary layer on steel under static conditions is described in order to prevent the running-in period with respect to the solution of ionic liquid in water. The best lubricating behaviour for the copper/copper contact and also for the steel/sapphire contact is obtained for the diprotic ammonium dianionic adipate, that has two carboxylate groups in its anion. A higher polarity and a higher number of ammonium protons, carboxylate and hydroxyl groups would give rise to stronger surface interaction with the metal surfaces and more stable boundary films. The tribological performance of new aprotic thiazolium ionic liquids and commercial aprotic imidazolium ionic liquids has been compared as lubricants of the steel/sapphire contact, obtaining the best results for the bis(trifluoromethanesulfonyl)imide derivatives, and also preventing tribocorrosion processes. The formation of a coating layer on magnesium alloys from phosphonate imidazolium ionic liquids by immersion and by chronoamperometry has been described. The new coatings reduce the abrasive wear in the magnesium-aluminium alloy but they are not effective in the magnesium-zinc alloy, which prevent the formation of continuous coatings. Los liquidos ionicos son sales liquidas a temperatura ambiente o bajas temperaturas que presentan excelentes propiedades fisico-quimicas. En el presente trabajo se estudian como lubricantes en problemas tribologicos complejos como la lubricacion de metales contra si mismos, el desarrollo de lubricantes base agua y de nuevas superficies autolubricadas. Cuando no es posible reducir la friccion y desgaste mediante lubricacion, como en las aleaciones de magnesio, los liquidos ionicos se han estudiado como precursores de recubrimientos protectores. Se han determinado las interacciones superficiales y los procesos de corrosion sobre cobre y sobre acero con diferentes liquidos ionicos proticos y aproticos para desarrollar nuevos lubricantes y aditivos. En el contacto cobre/cobre, excepto el liquido ionico protico derivado del oleato, todos los liquidos ionicos estudiados presentan mejor comportamiento tribologico que el lubricante comercial Polialfaolefina 6. En el contacto acero/zafiro, los nuevos liquidos ionicos proticos son buenos lubricantes cuando se utilizan en estado puro, y, como aditivos en agua, generan peliculas adsorbidas sobre la superficie del metal reduciendo la friccion y el desgaste tras la evaporacion del agua. Para evitar el periodo de alta friccion inicial en presencia de agua, se han generado peliculas superficiales de liquido ionico sobre el acero en condiciones estaticas. El mejor comportamiento lubricante tanto en el contacto cobre/cobre como en el contacto acero/zafiro se obtiene para el liquido ionico protico derivado del anion adipato, con dos grupos carboxilicos. Las interacciones de los grupos hidroxilo y carboxilato con la superficie metalica y los puentes de hidrogeno entre cation y anion podrian ser los responsables del buen comportamiento tribologico. Se ha comparado el comportamiento tribologico de los liquidos ionicos aproticos derivados de imidazolio y tiazolio como lubricantes del contacto acero/zafiro, obteniendose los mejores resultados para los derivados del anion bis(trifluorometanosulfonil)imida, que evita a su vez los procesos de tribocorrosion. Se han generado recubrimientos sobre aleaciones de magnesio con tres liquidos ionicos derivados del anion fosfonato, tanto por inmersion como mediante cronoamperometria. Los nuevos recubrimientos reducen el dano superficial por deslizamiento o abrasion de la aleacion magnesio-aluminio pero no de la aleacion magnesio-cinc, que impide la formacion de recubrimientos continuos.

  3. EU Del: exploring the onset of pulsation-driven winds in giant stars

    NASA Astrophysics Data System (ADS)

    McDonald, I.; Zijlstra, A. A.; Sloan, G. C.; Lagadec, E.; Johnson, C. I.; Uttenthaler, S.; Jones, O. C.; Smith, C. L.

    2016-03-01

    We explore the wind-driving mechanism of giant stars through the nearby (117 pc), intermediate-luminosity (L ≈ 1600 L⊙) star EU Del (HIP 101810, HD 196610). Atacama Pathfinder Experiment observations of the CO (3-2) and (2-1) transitions are used to derive a wind velocity of 9.51 ± 0.02 km s-1, a 12C/13C ratio of 14^{+9}_{-4} and a mass-loss rate of a few × 10-8 M⊙ yr-1. Analysis of published spectra show the star has a metallicity of [Fe/H] = -0.27 ± ˜0.30 dex. The star's dusty envelope lacks a clear 10-μm silicate feature, despite the star's oxygen-rich nature. Radiative transfer modelling cannot fit a wind acceleration model which relies solely on radiation pressure on condensing dust. We compare our results to VY Leo (HIP 53449), a star with similar temperature and luminosity, but different pulsation properties. We suggest the much stronger mass-loss from EU Del may be driven by long-period stellar pulsations, due to its potentially lower mass. We explore the implications for the mass-loss rate and wind velocities of other stars.

  4. Isolation of Sphaerotilus-Leptothrix strains from iron bacteria communities in Tierra del Fuego wetlands.

    PubMed

    Schmidt, Bertram; Snchez, Leandro A; Fretschner, Till; Kreps, Gastn; Ferrero, Marcela A; Sieriz, Faustino; Szewzyk, Ulrich

    2014-11-01

    Sheath-forming iron- and manganese-depositing bacteria belonging to the Sphaerotilus-Leptothrix group (SLG) are widespread in natural and artificial water systems. Known requirements for their growth include the presence of organic substrates and molecular oxygen. High concentrations of reduced iron or manganese, although not necessary for most species, make their growth a noticeable phenomenon. Such microbial communities have been studied mostly in the Northern Hemisphere. Here, we present descriptions of diverse ochre-depositing microbial communities in Tierra del Fuego, Argentina, using a combined approach of microscopical examination, clone library construction and cultivation focused on SLG bacteria. To date, only few SLG type strains are available. The present work increases the number and diversity of cultivated SLG bacteria by obtaining isolates from biofilms and sediment samples of wetlands in Tierra del Fuego. Thirty isolates were selected based on morphological features such as sheath formation and iron/manganese deposition. Five operational taxonomic units (OTUs) were deduced. Sequencing of 16S rRNA genes showed that one OTU is identical to the Leptothrix mobilis Feox-1(T) -sequence while the four remaining OTUs show similarity values related to previously described type strains. Similarity values ranged from 96.5% to 98.8%, indicating possible new species and subspecies. PMID:25098830

  5. The Last Glacial Maximum and Termination in the Torres del Paine Region, Southern South America

    NASA Astrophysics Data System (ADS)

    Garcia, J.; Hall, B. L.; Kaplan, M. R.; Vega, R. M.; Binnie, S.; Gmez, G.; Santana, F.

    2012-12-01

    Deciphering the timing, structure and termination of the local last glacial maximum (LGM) throughout Patagonia (42-55 S) remains one of the key unsolved paleoclimate questions in Quaternary sciences. During the last glaciation, the Patagonian ice sheet formed one ice body along the Patagonian Andes (42-55 S) in southern South America, but previous work has revealed different spatiotemporal ice dynamics along the eastern and western ice margins. The Patagonian Andes is the only landmass that exists at this latitude confronting the southern westerly wind belt, which seems to have played a key role in past glacial and climate changes. Therefore, reconstructing southern Andes glacier history constitutes a key element for understanding the causes of glaciations in the Southern Hemisphere. Major progress has been made to document the local Late-Pleistocene glacier history, particularly in response to recent application of exposure-cosmogenic dating technique in the region, although only sparse well-dated paleoclimate records exist in this vast area. LGM moraine-based records in south Patagonia (~48-55 S) have been developed for the Strait of Magellan area, where full glacial conditions seems to have occurred between ~28.0 - 17.5 ka. Despite that these data seem to confirm previous glacial chronologies developed in north Patagonia and the Chilean Lake District (40-42 S), recent works in Torres del Paine and ltima Esperanza basins (50-51 S), suggest that glacial maximum conditions may have occurred earlier (i.e., during Marine Isotope Stage 3) and that ice extent could have been twice the size of previously thought. Here, we discuss paleoclimatological implications from our 10Be and 26Al-dating program of moraines in the Torres del Paine region in southern Patagonia. We focused our efforts in the previously undated Ro de las Viscachas (RV) I and II moraines, which occur distal to the late-glacial TDP II, III and IV moraines that enclose present lake bodies at the Torres del Paine National Park (e.g., Lagos del Toro, Sarmiento and Azul). RV moraines parallel each other and can be tracked continuously for several km along the southern slope of Sierra Contreras in Chile. To the east, in present day Argentina, they constitute concentric wide moraine arcs that include tens of frontal moraine ridges. We collected boulders embedded in the relatively sharp RV I and II moraines for 10Be and 26Al cosmogenic-exposure dating to reconstruct the last glacial period. We also sampled and dated cobbles from the main glaciofluvial plain grading to the outer RV I landforms, to crosscheck with the cosmogenic ages obtained from boulders embedded at moraine tops. Additionally, we present new 10Be cosmogenic ages from boulders in TDP I and TDP IV moraines at Lago del Toro basin, which help us to define the structure of the local termination.

  6. L10P and P158DEL DJ-1 Mutations Cause Protein Instability, Aggregation, and Dimerization Impairments

    PubMed Central

    Ramsey, Chenere P.; Giasson, Benoit I.

    2010-01-01

    A variety of mutations in the gene encoding DJ-1 protein are causal of autosomal recessive early-onset parkinsonism. Recently, a novel pathogenic homozygous DJ-1 missense mutation resulting in the L10P amino acid substitution was reported. In a separate study, a novel homozygous mutation resulting in the deletion of DJ-1 residue P158 was also reported to be causative of disease. The specific effects of the novel L10P and P158DEL mutations on protein function have not been studied. Herein, L10P and P158DEL DJ-1 proteins were assessed for protein stability, dimerization, solubility, subcellular localization, and protective function in comparison to WT and the L166P DJ-1 pathogenic variant. It was discovered that in comparison to WT protein, L10P, L166P, and P158DEL DJ-1 variants exhibited dramatically reduced protein stabilities. Degradation of each of the respective pathogenic mutants appeared to be mediated in-part by the proteasome. Interestingly, unlike L166P DJ-1, the L10P and P158DEL DJ-1 variants retained the ability to dimerize with WT DJ-1 protein; however, neither of these mutants was able to form homodimers. Additionally, the L10P, L166P, and P158DEL DJ-1 variants exhibited altered profiles on size-exclusion chromatography and demonstrated reduced solubilities in comparison to WT protein, and the latter aberration could be exacerbated in the presence of MG-132. Further, cells stably expressing L10P DJ-1 were more vulnerable to treatments with proteasome inhibitors, suggesting that L10P DJ-1 may be toxic to cells under conditions of proteasome stress. Taken together, these findings suggest that diverse aberrant mechanisms, including alterations in protein stability and protein folding, are associated with the pathogenicity of the L10P and P158DEL DJ-1 variants. PMID:20806408

  7. Health assessment for Del Norte County Pesticide Storage Area, Cresent City, Del Norte County, California, Region 9. CERCLIS No. CAD000626176. Final report

    SciTech Connect

    Not Available

    1989-04-18

    The Del Norte County Pesticide Storage Area is located northwest of Cresent City, California. The site soils and ground water were contaminated with a myriad of pesticides and herbicides. The data also indicated an elevated concentration of chromium was present on-site and off-site; however, it does not appear to be related to the activities involving the use of the site as a pesticide storage area. The site was included on the National Priorities List in 1983. The storage area operated from 1970 until 1981, accepting containers from local agricultural and forestry-related industries. The intended use of the site was as an interim or emergency storage area for pesticide containers which had been triple rinsed and punctured prior to coming to the site. There were 9 private wells monitored within 0.50 miles of the site and the results indicated these wells were not contaminated by site contaminants. This site is of public health concern because of the potential for exposure to pesticides, herbicides, and chromium at concentrations of health concern.

  8. 77 FR 4897 - Safety Zone; M/V Del Monte Live-Fire Gun Exercise, James River, Isle of Wight, VA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-01

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; M/V Del Monte Live-Fire Gun Exercise, James... live-fire gun exercises on the M/V Del Monte. This action is intended to restrict vessel traffic movement to protect mariners from the hazards associated with the live-fire gun exercise. DATES: This...

  9. Aspectos generales de los exámenes de detección del cáncer (PDQ)—Versión para profesionales de salud

    Cancer.gov

    Información sobre la medición de la eficacia de las pruebas de detección del cáncer y la evaluación de la solidez de las pruebas obtenidas en estudios de investigación de detección del cáncer.

  10. Estudio del NCI revela que la obesidad extrema puede acortar la esperanza de vida hasta en 14 años

    Cancer.gov

    Los adultos con obesidad extrema tienen mayor riesgo de morir a edad más joven por cáncer y muchas otras causas entre ellas, enfermedades cardíacas, accidentes cerebrovasculares, diabetes y enfermedades del hígado y los riñones, según estudio del NCI.

  11. Aspectos generales de los exámenes de detección del cáncer (PDQ)—Versión para pacientes

    Cancer.gov

    Información sobre la medición de la eficacia de los exámenes de detección del cáncer y la evaluación de la solidez de las pruebas obtenidas en estudios de investigación de detección del cáncer.

  12. Centro para la Salud Mundial del NCI anuncia becas de investigación para tecnologías portátiles

    Cancer.gov

    El Centro para la Salud Mundial del NCI (CGH) anunció el otorgamiento de subvenciones que apoyarán el desarrollo y la validación de tecnologías portátiles y de bajo costo para mejorar la detección temprana, el diagnóstico y el tratamiento del cáncer.

  13. Genetic polymorphism in HLA-G 3'UTR 14-bp ins/del and risk of cancer: a meta-analysis of case-control study.

    PubMed

    Li, Tao; Huang, Haohai; Liao, Dan; Ling, Huahuang; Su, Bingguang; Cai, Maode

    2015-08-01

    Accumulating evidence has suggested that the human leucocyte antigen-G (HLA-G) 14 bp ins/del polymorphism might be related to cancer susceptibility. However, epidemiologic findings have been inconsistent. Therefore, we performed a meta-analysis of case-control study to derive a more precise estimation of this association. Electronic databases were searched to identify all eligible studies of HLA-G 14 bp ins/del polymorphism and cancer risk. Odds ratios (ORs) with 95 % confidence intervals (CIs) were calculated to evaluate the strength of the association in fixed-effects model or random-effects model according to heterogeneity. Publication bias, sensitivity analysis and subgroup analyses based on cancer type, ethnicity, source of controls and sample size were also performed. A total of 14 case-control studies, involving 2,757 cases and 3,972 controls, were included in the present meta-analysis. The pooled analysis showed that there is no significant relationship between the HLA-G 14 bp ins/del polymorphism and cancer susceptibility under the genetic models (for the allele model del vs. ins: OR 1.13, 95 % CI 1.00-1.27; for the homozygote comparison model del/del vs. ins/ins: OR 1.22, 95 % CI 0.95-1.56; for the dominant model del/del + ins/del vs. ins/ins: OR 1.15, 95 % CI 0.94-1.42; for recessive model del/del vs. ins/del + ins/ins: OR 1.13, 95 % CI 0.96-1.34; respectively). Subgroup analyses indicated significant association among breast cancer, population based control and the large sample size group in some genetic models. Our investigations demonstrate that genotypes for the HLA-G 14 bp ins/del polymorphism may be not associated with overall cancer risk. In a subgroup meta-analysis, however, HLA-G 14-bp ins/del polymorphism might contribute to breast cancer susceptibility. PMID:25573780

  14. LumacaftorIvacaftor in Patients with Cystic Fibrosis Homozygous for Phe508del CFTR

    PubMed Central

    Wainwright, C.E.; Elborn, J.S.; Ramsey, B.W.; Marigowda, G.; Huang, X.; Cipolli, M.; Colombo, C.; Davies, J.C.; De Boeck, K.; Flume, P.A.; Konstan, M.W.; McColley, S.A.; McCoy, K.; McKone, E.F.; Munck, A.; Ratjen, F.; Rowe, S.M.; Waltz, D.; Boyle, M.P.

    2016-01-01

    BACKGROUND Cystic fibrosis is a life-limiting disease that is caused by defective or deficient cystic fibrosis transmembrane conductance regulator (CFTR) protein activity. Phe508del is the most common CFTR mutation. METHODS We conducted two phase 3, randomized, double-blind, placebo-controlled studies that were designed to assess the effects of lumacaftor (VX-809), a CFTR corrector, in combination with ivacaftor (VX-770), a CFTR potentiator, in patients 12 years of age or older who had cystic fibrosis and were homozygous for the Phe508del CFTR mutation. In both studies, patients were randomly assigned to receive either lumacaftor (600 mg once daily or 400 mg every 12 hours) in combination with ivacaftor (250 mg every 12 hours) or matched placebo for 24 weeks. The primary end point was the absolute change from baseline in the percentage of predicted forced expiratory volume in 1 second (FEV1) at week 24. RESULTS A total of 1108 patients underwent randomization and received study drug. The mean baseline FEV1 was 61% of the predicted value. In both studies, there were significant improvements in the primary end point in both lumacaftorivacaftor dose groups; the difference between active treatment and placebo with respect to the mean absolute improvement in the percentage of predicted FEV1 ranged from 2.6 to 4.0 percentage points (P<0.001), which corresponded to a mean relative treatment difference of 4.3 to 6.7% (P<0.001). Pooled analyses showed that the rate of pulmonary exacerbations was 30 to 39% lower in the lumacaftorivacaftor groups than in the placebo group; the rate of events leading to hospitalization or the use of intravenous antibiotics was lower in the lumacaftorivacaftor groups as well. The incidence of adverse events was generally similar in the lumacaftorivacaftor and placebo groups. The rate of discontinuation due to an adverse event was 4.2% among patients who received lumacaftorivacaftor versus 1.6% among those who received placebo. CONCLUSIONS These data show that lumacaftor in combination with ivacaftor provided a benefit for patients with cystic fibrosis homozygous for the Phe508del CFTR mutation. (Funded by Vertex Pharmaceuticals and others; TRAFFIC and TRANSPORT ClinicalTrials.gov numbers, NCT01807923 and NCT01807949.) PMID:25981758

  15. Structure of Mesita del Buey at TA-54, Los Alamos National Laboratory, New Mexico

    SciTech Connect

    Reneau, S.L.; Broxton, D.E.; Carney, J.S.; LaDelfe, C.

    1998-04-23

    The geological structure of Mesita del Buey at Technical Area 54 (TA-54) was examined using precise surveying of the contact between units 1v and 2 of the Tshirege Member of the Bandelier Tuff at 3.5 km along the north wall of Pajarito Canyon and 0.6 km along the north wall of a tributary to Canada del Buey. Estimated structure contours on this contact indicate typical strikes of N40E to N70E along this part of Mesita del Buey, although the apparent strike of the tuff is E-W at the western part of the survey. Typical dips are 1.0{degree} to 2.0{degree} to the east or southeast, with an estimated maximum dip of 3.2{degree} near the west end of Material Disposal Area G. Thirty seven faults with vertical displacements of 5 to 65 cm were observed in outcrop along the Pajarito Canyon traverse, and, due to the incomplete exposure of the unit 1v-unit 2 contact, many more faults of this magnitude undoubtedly exist. The faults have a wide range in strike and have either down-to-the-west and down-to-the-east components of offset, although about 65% of the observed displacement is down-to-the-west or northwest. These faults are not clearly associated with major fault zones, indicated by the general absence of larger-scale offsets or inflections along the unit 1v-unit 2 contact in areas where the small-scale faults were observed; they may instead record distributed secondary deformation across the Pajarito Plateau associated with large earthquakes on the main Pajarito fault zone 8--11 km to the west, or perhaps other faults in the region. The survey data also suggest that a 150--250 m wide zone of greater magnitude faulting is present near the west end of the traverse associated with a horst-and-graben structure displaying about 1.5--3.5 m of offset on individual faults, although the total amount of offset across this structure and its orientation are not known.

  16. Neurofibromatosis 1 prevalence in children aged 9-11 years, Pinar del Ro Province, Cuba.

    PubMed

    Orraca, Miladys; Morejn, Griselda; Cabrera, Niurka; Menndez, Reinaldo; Orraca, Odalys

    2014-01-01

    INTRODUCTION Neurofibromatosis 1 is one of the most common heritable genetic disorders in humans. It is characterized by formation of neurofibromas, with marked variability in expression. Half the cases are due to autosomal dominant inheritance; the rest arise from de novo mutations. Prevalence varies by population, and prevalence in Cuba is unknown. OBJECTIVE Determine the prevalence of neurofibromatosis 1 in a population of Cuban children aged 9-11 years old in Pinar del Ro Province, Cuba. METHODS A descriptive cross-sectional study was carried out in Pinar del Ro Province in 2004, in which 19,392 children were assessed for neurofibromatosis 1. The study was conducted in two phases: the first, a survey of the entire population aged 9-11 years by genetic counselors in the province's schools; the second, assessment by clinical geneticists of children who met criteria for referral to the Provincial Medical Genetics Center. Neurofibromatosis 1 cases and first-degree relatives were examined to identify the origin of the mutation (de novo or inherited). Neurofibromatosis 1 prevalence was calculated, as well as history of a first-degree relative with the disease and frequency of several principal clinical signs-caf au lait spots, freckles in places unexposed to sunlight, presence of neurofibromas, Lisch nodules and characteristic bone lesions. RESULTS Of the eligible population, 99.3% was screened (10,034 boys and 9358 girls). Active case finding resulted in referral of 200 children to medical geneticists and the disease was confirmed in 17, for a prevalence of one case per 1141 children aged 9-11 years old. Caf au lait spots were the most frequent sign (100%), followed by freckles in areas unexposed to sunlight (82.4%) and characteristic bone lesions (41.2%). Only 4 of the 17 cases were previously being treated for the disease. CONCLUSIONS Neurofibromatosis 1 has high prevalence in the group studied in Pinar del Rio Province and most cases are not detected in primary health care settings. PMID:25208116

  17. Application of GIS and remote sensing methods to the paleohydrography of the Paso del Norte region

    NASA Astrophysics Data System (ADS)

    Kennedy, John F.

    The purpose of this project is to use geographic information system (GIS) and remote sensing methods to investigate the paleohydrology of the Paso del Norte region during the latest pluvial event. During the research I gathered the best available geologic and hydrographic data sets and integrated these data within a GIS in order to create a digital paleohydrologic database for the region. In particular, I used the GIS to reconstruct the hydrography (i.e. streams, lakes, and watershed boundaries) that existed as a results of increased precipitation and a decrease in temperature in the recent geologic past. The existence of the pluvial lakes had an impact on ground-water and surface-water flow systems in this region. The results of my research are presented as two papers ready for peer-review publication submission and a third paper that is a previously published work. (1) Using GIS and Remote Sensing to Reconstruct Late Quaternary and Early Holocene Paleo-Hydrography Using Climate Data and Modern Topography---an Example from the Southwestern US. This paper describes the methods used to create a paleo-hydrography system for the Paso del Norte region, and the development of a water-balance equation that estimates the inputs and outputs of this system. (2) Estimating Water-Balance Equation for Playas in the Tularosa Basin of Southern New Mexico. This paper describes the calibration and testing of the GIS model presented in the first paper. Before it can be applied to paleohydrologic systems, it must be shown to reasonably approximate playa systems in the region under current conditions. Water balances were calculated for several playas and their associated drainages. (3) The Hydrogeologic Framework of Basin-Fill Aquifers and Associated Ground-water flow Systems in Southwestern New Mexico---An Overview. The paper is an overview of work done by many hydrogeologists in the Paso del Norte region; however, the primary source of most of our current knowledge is based on the lifetime of work supplied by this paper's co-author: John W. Hawley. (Abstract shortened by UMI.)

  18. Early evolution of the extraordinary Nova Delphini 2013 (V339 Del)

    NASA Astrophysics Data System (ADS)

    Skopal, A.; Drechsel, H.; Tarasova, T.; Kato, T.; Fujii, M.; Teyssier, F.; Garde, O.; Guarro, J.; Edlin, J.; Buil, C.; Antao, D.; Terry, J.-N.; Lemoult, T.; Charbonnel, S.; Bohlsen, T.; Favaro, A.; Graham, K.

    2014-09-01

    Aims: We determine the temporal evolution of the luminosity (LWD), radius (RWD) and effective temperature (Teff) of the white dwarf (WD) pseudophotosphere of V339 Del from its discovery to around day 40. Another main objective was studying the ionization structure of the ejecta. Methods: These aims were achieved by modelling the optical/near-IR spectral energy distribution (SED) using low-resolution spectroscopy (3500-9200 Å), UBVRCIC and JHKLM photometry. Important insights in the physical conditions of the ejecta were gained from an analysis of the evolution of the Hα and Raman-scattered 6825 Å O vi line using medium-resolution spectroscopy (R ~ 10 000). Results: During the fireball stage (Aug. 14.8-19.9, 2013), Teff was in the range of 6000-12 000 K, RWD was expanding non-uniformly in time from ~66 to ~300 (d/ 3 kpc) R⊙, and LWD was super-Eddington, but not constant. Its maximum of ~9 × 1038 (d/ 3 kpc)2 erg s-1 occurred around Aug. 16.0, at the maximum of Teff, half a day before the visual maximum. After the fireball stage, a large emission measure of 1.0-2.0 × 1062 (d/ 3 kpc)2 cm-3 constrained the lower limit of LWD to be well above the super-Eddington value. The mass of the ionized region was a few × 10-4 M⊙, and the mass-loss rate was decreasing from ~5.7 (Aug. 22) to ~0.71 × 10-4 M⊙ yr-1 (Sept. 20). The evolution of the Hα line and mainly the transient emergence of the Raman-scattered O vi 1032 Å line suggested a biconical ionization structure of the ejecta with a disk-like H i region persisting around the WD until its total ionization, around day 40. On Sept. 20 (day 35), the model SED indicated a dust emission component in the spectrum. The dust was located beyond the H i zone, where it was shielded from the hard, ≳105 K, radiation of the burning WD at that time. Conclusions: Our extensive spectroscopic observations of the classical nova V339 Del allowed us to map its evolution from the very early phase after its explosion. It is evident that the nova was not evolving according to the current theoretical prediction. The unusual non-spherically symmetric ejecta of nova V339 Del and its extreme physical conditions and evolution during and after the fireball stage represent interesting new challenges for the theoretical modelling of the nova phenomenon. Based on data collected by amateur astronomers.

  19. Geology and depositional environments of the Guadalupian rocks of the northern Del Norte Mountains, West Texas

    USGS Publications Warehouse

    Rudine, S.F.; Wardlaw, B.R.; Rohr, D.M.; Grant, R.E.

    2000-01-01

    The Guadalupian rocks of the northern Del Norte Mountains were deposited in a foreland basin between land of the Marathon orogen and a carbonate shoal established on the geanticline separating the foreland basin from the Delaware basin. Deposition was alternately influenced by coarse clastic input from the orogen and carbonate shoal, which interrupted shallow basinal siltstone depletion. Relatively deeper-water deposition is characterized by carbonate input from the shoal, and relatively shallow-water deposition is characterized by sandstone input from the orogen. Deposition was in five general transgressive-regressive packages that include (1) the Road Canyon Formation and the first siltstone member and first sandstone member of the Word Formation, (2) the second siltstone member, Appel Ranch Member, and limy sandy siltstone member of the Word Formation, (3) the Vidrio Formation, (4) the lower and part of the middle members of the Altuda Formation, and (5) part of the middle and upper members of the Altuda Formation.

  20. Dynamic response to valley breeze circulation in Santa Mara del Oro, a volcanic lake in Mexico

    NASA Astrophysics Data System (ADS)

    Serrano, David; Filonov, Anatoliy; Tereshchenko, Irina

    2002-07-01

    The paper discusses the dynamic response to valley breeze circulation in Santa Maria del Oro, a volcanic lake in Mexico. Hourly records of wind measurements were used to construct a hydrodynamical model of the level fluctuations of the lake's water as well as integrated drift currents. The calculations show that the valley breeze circulation stimulates barotropic seiches in the lake with a period of 2.6 minutes and maximal level in the southwest part up to 18 mm. The drift current form two circulating rings having an opposite directions: anticyclonical in northern part of the lake and cyclonical in the southern. At the external edge of these rings the current speed can reach 20 cm/s. The measurements have shown that most part of the year the lake is strongly stratified. The maximal vertical temperature gradients are over 1C/m in November and August, in a layer of 17-20 m.

  1. Discovering research value in the Campo del Cielo, Argentina, meteorite craters

    NASA Astrophysics Data System (ADS)

    Cassidy, William A.; Renard, Marc L.

    1996-07-01

    The Campo del Cielo meteorite crater field in Argentina contains at least 20 small meteorite craters, but a recent review of the field data and a remote sensing study suggest that there may be more. The fall occurred 4000 years ago into a uniform loessy soil, and the craters are well enough preserved so that some of their parameters of impact can be determined after excavation. The craters were formed by multi-ton fragments of a type IA meteoroid with abundant silicate inclusions. Relative to the horizontal, the angle of infall was 9. Reflecting the low angle of infall, the crater field is elongated with apparent dimensions of 3 18.5 km. The largest craters are near the center of this ellipse. This suggests that when the parent meteoroid broke apart, the resulting fragments diverged from the original trajectory in inverse relation to their masses and did not undergo size sorting due to atmospheric deceleration. The major axis of the crater field as we know it extends along N63E, but the azimuths of infall determined by excavation of Craters 9 and 10 are N83.5E and N75.5E, respectively. This suggests that the major axis of the crater field is not yet well determined. The three or four largest craters appear to have been formed by impacts that disrupted the projectiles, scattering fragments around the outsides of the craters and leaving no large masses within them; these are relatively symmetrical in shape. Other craters are elongated features with multi-ton masses preserved within them and no fragmentation products outside. There are two ways in which field research on the Campo del Cielo crater field is found to be useful. (1) Studies exist that have been used to interpret impact craters on planetary surfaces other than the Earth. This occurrence of a swarm of projectiles impacting at known angles and similar velocities into a uniform target material provides an excellent field site at which to test the applicability of those studies. (2) Individual craters at Campo del Cielo can yield the masses of the projectiles that formed them and their velocities, angles and azimuths of impact. From these data, there is a possibility to estimate parameters for the parent meteoroid at entry and, thus, learn enough about its orbit to judge whether or not it was compatible with an asteroidal origin. Preliminary indications are that it was. Campo del Cielo is a IA iron meteorite and Sikhote-Alin, an observed fall, is a IIB iron meteorite in Wasson's classification. The Sterlitamak iron, also an observed fall, is a medium octahedrite in the Prior-Hey classification. It would be interesting to compare their orbital parameters.

  2. Novel Vascular Malformation in an Affected Newborn with Deletion Del(4)(q31.3)

    PubMed Central

    de Len Ojeda, Norma Elena; Soriano-Torres, Michel; Cabrera, Mercedes J.; Bentez Ramos, Dunia Brbara

    2012-01-01

    We report on a newborn male patient with a terminal deletion in the long arm of the chromosome 4 with a congenital heart defect unreported before in association with this syndrome. The patient had multiple congenital anomalies including a pointed duplicated fingernail, low set posteriorly rotated ears, large anterior fontanel, micrognathia, glabellar capillary vascular malformation, and Interrupted Aortic Arch type C. The patient died due to multiple congenital malformations; a peripheral chromosome analysis showed 46, XY, del(4)(q31.3) de novo. The only reported case with the same deletion was a male newborn that exhibited the pattern of minor anomalies of deletion 4q31 syndrome. The parents were cytogenetically normal. We compare clinical signs to other cases with a deletion in long arm of chromosome 4. PMID:23320208

  3. [VI Ibero-American Summit of heads of state and government: Declaration of Via del Mar].

    PubMed

    1997-09-01

    The leaders of 21 Ibero-American countries came to Via del Mar (Chile) for the sixth meeting of the now-traditional Ibero-American Summits. The Declaration that resulted from the Summit reiterated the commitment of these nations to the principles of democracy, the rule of law, and political pluralism. The development of efficient and participatory systems of democratic government was one of the most important of the topics dealt with at the meeting and served as the framework for the discussion and the search for solutions to common problems, such as poverty, arms trafficking, unequal opportunity for men and women, and inappropriate government practices. The leaders also supported a series of initiatives and international programs in the technological and cultural arenas that will strengthen the ties and unite the Spanish- and Portuguese-speaking countries. PMID:9445767

  4. PaDEL-DDPredictor: open-source software for PD-PK-T prediction.

    PubMed

    He, Yuye; Liew, Chin Yee; Sharma, Nitin; Woo, Sze Kwang; Chau, Yi Ting; Yap, Chun Wei

    2013-03-15

    ADMET (absorption, distribution, metabolism, excretion, and toxicity)-related failure of drug candidates is a major issue for the pharmaceutical industry today. Prediction of PD-PK-T properties using in silico tools has become very important in pharmaceutical research to reduce cost and enhance efficiency. PaDEL-DDPredictor is an in silico tool for rapid prediction of PD-PK-T properties of compounds from their chemical structures. It is free and open-source software that, has both graphical user interface and command line interface, can work on all major platforms (Windows, Linux, and MacOS) and supports more than 90 different molecular file formats. The software can be downloaded from http://padel.nus.edu.sg/software/padelddpredictor. PMID:23114987

  5. [Historical notes about scientific research in the Instituto Mexicano del Seguro Social.

    PubMed

    Zrate, Arturo; Basurto-Acevedo, Lourdes

    2013-01-01

    Medical research in the Instituto Mexicano del Seguro Social has been considered one of the most important in this country for quality and quantity. Thanks to the work and leadership of Benito Coquet, who initiated the building of the National Medical Center in 1961, and the work of two pillars of research, Luis Castelazo and Bernardo Seplveda, the Institute successfully improved scientific research. In the years that followed, the Institute fostered the professionalization of research, the creation of research units in different areas of science, the incorporation of consolidated groups of researchers, the relationship with other institutions, the incorporation to the Sistema Nacional de Investigadores, the editing of a journal to expose outside the work done within the Institute, and the formation of a trust to raise funds for financing. Thanks to all that, institutional research strengthened in all lines, and it was placed first, at certain times, at the national level. PMID:24290017

  6. Radiogenic Xenon-129 in Silicate Inclusions in the Campo Del Cielo Iron Meteorite

    NASA Technical Reports Server (NTRS)

    Meshik, A.; Kurat, G.; Pravdivtseva, O.; Hohenberg, C. M.

    2004-01-01

    Iron meteorites present a challenge for the I-Xe dating technique because it is usually the inclusions, not metal, that contain radiogenic xenon and iodine. Silicate inclusions are frequent in only types IAB and IIE, and earlier studies of irons have demonstrated that I-Xe system can survive intact in these inclusions preserving valuable age information. Our previous studies of the I-Xe record in pyroxene grains from Toluca iron suggested an intriguing relationship between apparent I-Xe ages and (Mg+Fe)/Fe ratios. The I-Xe system in K-feldspar inclusions from Colomera (IIE) had the fingerprint of slow cooling, with an indicated cooling rate of 2-4 C/Ma. Here we present studies of the iodine-xenon system in a silicate-graphite-metal (SiGrMet) inclusion of the IA Campo del Cielo iron meteorite from the collection of the Museum of Natural History in Vienna.

  7. Geophysical characterization of two circular structures at Bajada del Diablo (Patagonia, Argentina): Indication of impact origin

    NASA Astrophysics Data System (ADS)

    Prezzi, Claudia B.; Orgeira, María Julia; Acevedo, Rogelio D.; Ponce, Juan Federico; Martinez, Oscar; Rabassa, Jorge O.; Corbella, Hugo; Vásquez, Carlos; González-Guillot, Mauricio; Subías, Ignacio

    2012-02-01

    An impact origin has been proposed for the circular structures found in Bajada del Diablo, Patagonia, Argentina. Taking into account its extension and the number of impact structures, Bajada del Diablo would be the largest meteoritic impact areas known on Earth, being an extremely interesting area for the research of impact events and processes. Moreover, the global distribution of known impact structures shows a surprising asymmetry. Particularly, South America has only seven described areas. It is evident that this situation is an artifact, highlighting the importance of intensifying the research in the least studied areas such as Argentina. Circular structures in Bajada del Diablo have been identified on two rock types: the Quiñelaf eruptive complex and Pampa Sastre Formation. In the first case, circular structures are placed in olivine basalts. On the other hand, Pampa Sastre Formation (late Pliocene/early Pleistocene) corresponds to conglomerate layers with basalt clasts boulder and block in size in a coarse sandy matrix. With the aim of further the investigation of the proposed impact origin for these circular structures, we carried out detailed topographic, magnetic and electromagnetic ground surveys in two circular structures ("8" and "A") found in Pampa Sastre conglomerates. Both circular structures are simple, bowl-shaped with rim diameters of 300 m and maximum depths of 10 m. They have been partially filled in by debris flows from the rims and wind-blown sands. Two preliminary magnetic profiles have also been carried out in circular structure "G" found in Quiñelaf basalts. The magnetic anomalies show a circular pattern with a slightly negative and relatively flat signal in the circular structures' bases. Furthermore in the circular structures' rims, high-amplitude, conspicuous and localized (short wavelength) anomalies are observed. Such large amplitude and short wavelength anomalies are not detected outside the circular structures. For all used frequencies, the electromagnetic profiles show lower apparent electrical conductivities in the circular structures' base, while the rims present notably higher values. Curvature attributes, analytic signal, horizontal gradient and Euler solutions were calculated for the magnetic data. 2.5D magnetic models were developed across the studied circular structures. Our results suggest that in the circular structures' bases up to 12 m of Pampa Sastre conglomerate would have been removed. On the contrary, the circular structures' rims exhibit high-amplitude, localized magnetic anomalies and higher apparent electrical conductivities, which would be related to the anomalous accumulation of basalt boulders and blocks remanently magnetized. Such high-amplitude anomalies are not present outside the surveyed circular structures. The geomorphological, geological and geophysical features of the studied circular structures can only be explained by means of an extra-terrestrial projectile impact. We conclude that, considering the results obtained to date, Bajada del Diablo should be envisaged as a focus of further research, which could provide novel information about impact events, associated processes and their evidences. Particularly, the data produced in this study could represent one of the first documented geophysical signatures of the impact of a comet nucleus on Earth.

  8. Modelo analítico del efecto de PRS sobre satélites GPS

    NASA Astrophysics Data System (ADS)

    Meza, A.; Brunini, C.; Usandivaras, J. C.

    El sistema GPS (Global Position System) es, hoy en día, la herramienta de navegación y posicionamiento más potente y lo será sin duda en la próxima década. Gran parte de su valiosa utilidad se debe a la alta precisión que permite lograr y ésta, a su vez, depende, entre otras causas, de la precisión con que se conocen las órbitas de los satélites. La presión de radiación solar (PRS) fija el límite de la precisión con que pueden calcularse en la actualidad las efemérides satelitarias. El objetivo de este trabajo es proponer una mejor resolución de este fenómeno. El modelo analítico aquí presentado, se basa en el análisis del comportamiento de los residuos de un ajuste por mínimos cuadrados en el que se utiliza el modelo de PRS propuesto por Beutler. El mismo consiste en un modelo determinista del fenómeno con dos parámetros libres. Los resultados obtenidos ponen de manifiesto que, aún después de aplicar dichos parámetros, prevalecen en los residuos efectos semidiurnos en las componentes radial,tangencial y normal. Estos resultados obtenidos se comparan con los de un trabajo desarrollado por el Instituto de Berne (Beutler et al., 1994), en el que se utilizaron como pseudo-observaciones las órbitas precisas del IGS (CODE). El intervalo de integración escogido por este centro fueron las semanas 680 y 681. En resumen se tienen arcos de 14 días para todos los satélites, donde las efemérides precisas de los mismos para los 14 días fueron utilizados como pseudo-observaciones. El modelo de fuerza que empleó dicho centro fue básicamente el tradicional en lo que respecta al modelo de las fuerzas gravitacionales, y para la PRS utilizo el modelo standard de Beutler. Los parámetros de este modelo junto con las 6 condiciones iniciales (posición y velocidad) fueron ajustados por el método general de mínimos cuadrados. Los residuos en la componente radial, tangencial y normal, para los satélites con un buen comportamiento, presentan una componente semidiurna. El modelo analítico planteado en este trabajo, predice el comportamiento de los residuos que se observan en las publicaciones más recientes. Esto abre el camino para plantear una estimación distinta de las incógnitas del problema, basado en el método de colocación por mínimos cuadrados. Ello requiere modelar estadísticamente la señal debida a las componentes de la PRS que no son tomadas en cuenta en el modelo determinista.

  9. RELACIÓN MÉDICO PACIENTE: DERECHOS DEL ADULTO MAYOR

    PubMed Central

    Barrantes-Monge, Melba; Rodríguez, Eduardo; Lama, Alexis

    2009-01-01

    Existen prejuicios en relación con la vejez, incluso entre los profesionales que se dedican a la gerontología. Uno común y peligroso es considerar que los viejos son todos enfermos o discapacitados. La relación médico-paciente es la piedra angular de la práctica y ética médicas. Para alcanzar el respeto por los adultos mayores es necesaria una medicina prudente, basada en una práctica en la cual la reflexión ética y clínica pueda contribuir. Esto último es posible si se hacen valer los derechos del adulto mayor, en particular como paciente para la toma de decisiones. PMID:20379380

  10. Heavy metal concentrations in litteral sediments from the Beagle Channel, Tierra del Fuego, Argentina.

    PubMed

    Amin, O; Ferrer, L; Marcovecchio, J

    1996-07-01

    For the first time the concentration of trace metals (Fe, Pb, Cu, Zn, Cd and total Hg) of sediments from the coastal zone of the Beagle Channel (Tierra del Fuego, in Southern Argentina) were measured. Atomic absorption spectrophotometry was utilized in order to determine the metal contents. The level of metals as observed in the sediments was recognized as the natural background, even though the use of normalization of lead, copper, and zinc to iron allowed the identification of the main sources of metal pollution for this environment. In order to develop future environmental monitoring programmes for the area of Ushuaia city and the Beagle Channel, the present results need to be considered. PMID:24193396

  11. Saprolegnia oliviae sp. nov. isolated from an Argentine river (Tierra del Fuego Province, Argentina).

    PubMed

    Steciow, Mnica Mirta

    2003-02-28

    Saprolegnia oliviae sp. nov. is described from litter (floating dead twigs, leaves and roots) in the Olivia River, Ushuaia Department, Tierra del Fuego Province (Argentina). The new species is illustrated and compared with other species of the genus. Distinguishing characteristics of S. oliviae are the production of smooth oogonia (with some lateral or terminal projections) and the absence of antheridial branches on the majority of the oogonia, but when present, they are mostly diclinous, at times oogonia are supplied with androgynous and monoclinous antheridial branches. The oogonial stalks are predominantly short and straight or long and bent, curved or many times coiled; oospores are distinctive subcentric, (1-) 15-50 (-70) per oogonium. Morphological details of the new species and its comparison with other described species are discussed here. PMID:12620629

  12. The DrosDel Deletion Collection: A Drosophila Genomewide Chromosomal Deficiency Resource

    PubMed Central

    Ryder, Edward; Ashburner, Michael; Bautista-Llacer, Rosa; Drummond, Jenny; Webster, Jane; Johnson, Glynnis; Morley, Terri; Chan, Yuk Sang; Blows, Fiona; Coulson, Darin; Reuter, Gunter; Baisch, Heiko; Apelt, Christian; Kauk, Andreas; Rudolph, Thomas; Kube, Maria; Klimm, Melanie; Nickel, Claudia; Szidonya, Janos; Mary, Peter; Pal, Margit; Rasmuson-Lestander, sa; Ekstrm, Karin; Stocker, Hugo; Hugentobler, Christoph; Hafen, Ernst; Gubb, David; Pflugfelder, Gert; Dorner, Christian; Mechler, Bernard; Schenkel, Heide; Marhold, Joachim; Serras, Florenci; Corominas, Montserrat; Punset, Adri; Roote, John; Russell, Steven

    2007-01-01

    We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering ?77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions. PMID:17720900

  13. 3 CFR 8946 - Proclamation 8946 of March 25, 2013. Establishment of the Río Grande del Norte National Monument

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... projectile points, as well as large ceramic vessels. The area is home to a rich array of archaeological... is known as the Río Grande del Norte, and its extraordinary array of scientific and historic... Apache and Ute Tribes, and hosts a dazzling array of rock art. Carved into the boulders and cliffs...

  14. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... River to Chesapeake Bay, Del. and Md., between Reedy Point, Delaware River, and Old Town Point Wharf... vessels using the waterway. The District Engineer's representative is the Chesapeake City Resident Engineer. The Chesapeake City Resident Engineer through the dispatcher on duty will enforce...

  15. Attic dust analysis approach for evaluation of heavy metal deposition in the El Paso Del Norte Region

    Technology Transfer Automated Retrieval System (TEKTRAN)

    During the 90 years from 1887 to 1977, a large smelter in the El Paso Del Norte region of North America smelted many ores including copper, lead, and zinc. In order to identify the patterns of heavy metal dispersion from the smelter, we sampled attic dust and dust from undisturbed surfaces in 15 bu...

  16. Strategic Planning for Institutions of Higher Education: A Content Analysis for the Universidad Tecnica del Estado Planning System.

    ERIC Educational Resources Information Center

    Karadima, Oscar

    Ten-year development plans of each of the eight campuses of the Universidad de Santiago de Chile, formerly called Universidad Tecnica del Estado, are evaluated, using content analysis. In addition to narrative descriptions, diagrams illustrate the features of each plan, which covers the period 1983-1993. Topics covered by the plans were grouped

  17. The Opera del Vocabolario Italiano Database: Full-Text Searching Early Italian Vernacular Sources on the Web.

    ERIC Educational Resources Information Center

    DuPont, Christian

    2001-01-01

    Introduces and describes the functions of the Opera del Vocabolario Italiano (OVI) database, a powerful Web-based, full-text, searchable electronic archive that contains early Italian vernacular texts whose composition may be dated prior to 1375. Examples are drawn from scholars in various disciplines who have employed the OVI in support of their

  18. Correction of human phospholamban R14del mutation associated with cardiomyopathy using targeted nucleases and combination therapy

    PubMed Central

    Karakikes, Ioannis; Stillitano, Francesca; Nonnenmacher, Mathieu; Tzimas, Christos; Sanoudou, Despina; Termglinchan, Vittavat; Kong, Chi-Wing; Rushing, Stephanie; Hansen, Jens; Ceholski, Delaine; Kolokathis, Fotis; Kremastinos, Dimitrios; Katoulis, Alexandros; Ren, Lihuan; Cohen, Ninette; Gho, Johannes M.I.H.; Tsiapras, Dimitrios; Vink, Aryan; Wu, Joseph C.; Asselbergs, Folkert W.; Li, Ronald A.; Hulot, Jean-Sebastien; Kranias, Evangelia G.; Hajjar, Roger J.

    2015-01-01

    A number of genetic mutations is associated with cardiomyopathies. A mutation in the coding region of the phospholamban (PLN) gene (R14del) is identified in families with hereditary heart failure. Heterozygous patients exhibit left ventricular dilation and ventricular arrhythmias. Here we generate induced pluripotent stem cells (iPSCs) from a patient harbouring the PLN R14del mutation and differentiate them into cardiomyocytes (iPSC-CMs). We find that the PLN R14del mutation induces Ca2+ handling abnormalities, electrical instability, abnormal cytoplasmic distribution of PLN protein and increases expression of molecular markers of cardiac hypertrophy in iPSC-CMs. Gene correction using transcription activator-like effector nucleases (TALENs) ameliorates the R14del-associated disease phenotypes in iPSC-CMs. In addition, we show that knocking down the endogenous PLN and simultaneously expressing a codon-optimized PLN gene reverses the disease phenotype in vitro. Our findings offer novel strategies for targeting the pathogenic mutations associated with cardiomyopathies. PMID:25923014

  19. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and navigation. 207.100 Section 207.100 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION...

  20. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and navigation. 207.100 Section 207.100 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION...

  1. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and navigation. 207.100 Section 207.100 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION...

  2. Cmo interpretar los nmeros: lo que verdaderamente nos dicen las estadsticas de los exmenes de deteccin del cncer

    Cancer.gov

    Los estudios sobre los exmenes de deteccin del cncer han mostrado que la realizacin de una mayor cantidad de exmenes no se traduce necesariamente en un menor nmero de muertes por cncer. Este artculo explica cmo interpretar las estadsticas utilizadas para describir los resultados de los exmenes de deteccin.

  3. Disseminated Medulloblastoma in a Child with Germline BRCA2 6174delT Mutation and without Fanconi Anemia

    PubMed Central

    Xu, Jingying; Margol, Ashley Sloane; Shukla, Anju; Ren, Xiuhai; Finlay, Jonathan L.; Krieger, Mark D.; Gilles, Floyd H.; Couch, Fergus J.; Aziz, Meraj; Fung, Eric T.; Asgharzadeh, Shahab; Barrett, Michael T.; Erdreich-Epstein, Anat

    2015-01-01

    Medulloblastoma, the most common malignant brain tumor in children, occurs with increased frequency in individuals with Fanconi anemia who have biallelic germline mutations in BRCA2. We describe an 8-year-old child who had disseminated anaplastic medulloblastoma and a deleterious heterozygous BRCA2 6174delT germline mutation. Molecular profiling was consistent with Group 4 medulloblastoma. The posterior fossa mass was resected and the patient received intensive chemotherapy and craniospinal irradiation. Despite this, the patient succumbed to a second recurrence of his medulloblastoma, which presented 8?months after diagnosis as malignant pleural and peritoneal effusions. Continuous medulloblastoma cell lines were isolated from the original tumor (CHLA-01-MED) and the malignant pleural effusion (CHLA-01R-MED). Here, we provide their analyses, including in vitro and in vivo growth, drug sensitivity, comparative genomic hybridization, and next generation sequencing analysis. In addition to the BRCA2 6174delT, the medulloblastoma cells had amplification of MYC, deletion at Xp11.2, and isochromosome 17, but no structural variations or overexpression of GFI1 or GFI1B. To our knowledge, this is the first pair of diagnosis/recurrence medulloblastoma cell lines, the only medulloblastoma cell lines with BRCA2 6174delT described to date, and the first reported case of a child with medulloblastoma associated with a germline BRCA2 6174delT who did not also have Fanconi anemia. PMID:26380221

  4. Mineralogy of Inclusions in the Campo Del Cielo and Mont Dieu Irons and Segregation of Partial Melts

    NASA Astrophysics Data System (ADS)

    Takeda, H.; Ishii, T.; Otsuki, M.

    2001-03-01

    To find partial melt segregation, we studied two new irons. Segregations of albite and chromite were found in Campo del Cielo and ameboidal FeS in Mont Dieu with the same shape as in IIE irons together with minor Cr diopside and Na,K-rich glass.

  5. Characterization of a mutant form of human apolipoprotein B (Thr26_Tyr27del) associated with familial hypobetalipoproteinemia.

    PubMed

    Magnolo, Lucia; Noto, Davide; Cefalù, Angelo B; Averna, Maurizio; Calandra, Sebastiano; Yao, Zemin; Tarugi, Patrizia

    2016-04-01

    We have previously identified a deletion mutant of human apoB [apoB (Thr26_Tyr27del)] in a subject with primary hypobetalipoproteinemia. The present study determined the effect of Thr26_Tyr27del mutation on apoB secretion using transfected McA-RH7777 cells. Transient or stable transfection of apoB-48 containing the Thr26_Tyr27del mutation showed drastically reduced secretion of the mutant as compared to wild-type apoB-48. No lipoproteins containing the mutant apoB-48 were secreted into the medium. Incubation of transfected cells in a lipid-rich medium in the presence of cycloheximide showed rapid turnover of cell-associated mutant apoB-48 as compared to that of wild-type apoB-48. Immunofluorescence experiments showed that the mutant apoB-48 was mostly localized in the endoplasmic reticulum. Treatment with the proteasomal inhibitor MG132 markedly attenuated the turnover of cell-associated mutant apoB-48, whereas treatment with inhibitors of autophagosomal/lysosomal function (e.g. 3-MA or ammonium chloride) had no effect. Taken together, these results indicated that the defective secretion of the Thr26_Tyr27del mutant was associated with increased intracellular degradation of apoB through the proteasome-dependent pathway. PMID:26825690

  6. The Constitutive Force of the "Catecismo del Pueblo" in Puerto Rico's Popular Democratic Party Campaign of 1938-1940

    ERIC Educational Resources Information Center

    Cordova, Nathaniel I.

    2004-01-01

    This paper explores the formation of a political order, a constituency, during the 1938-1940 senatorial campaign of the "Partido Popular Democratico" in Puerto Rico. In particular it examines the constitutive force of the "Catecismo del Pueblo," a small booklet in the form of questions and answers regarding the party's basic assumptions and

  7. Informe Anual a la Nación sobre el Estado del Cáncer, 1975 a 2012

    Cancer.gov

    El Informe Anual a la Nación sobre el Estado del Cáncer, 1975 a 2012, es una actualización de los índices de casos nuevos, muertes y tendencias de los cánceres más comunes en los Estados Unidos.

  8. Análisis del antígeno prostático específico (PSA)

    Cancer.gov

    Hoja informativa sobre la prueba del antígeno prostático específico (PSA) y los exámenes selectivos de detección para el cáncer de próstata, y clarifica los beneficios y limitaciones de dicha prueba.

  9. Informe Anual a la Nacin sobre el Estado del Cncer con una seccin especial sobre la prevalencia d

    Cancer.gov

    El Informe Anual a la Nacin sobre el Estado del Cncer (1975 a 2010), mostr un descenso ms acelerado que en aos anteriores de los ndices de mortalidad por cncer de pulmn. Tambin contiene una seccin especial que destaca los efectos significativos

  10. Developing Flexible Dual Master's Degree Programs at UPAEP (Universidad Popular Autonoma del Estado de Puebla) and OSU (Oklahoma State University)

    ERIC Educational Resources Information Center

    Fabregas-Janeiro, Maria G.; de la Parra, Pablo Nuno

    2012-01-01

    In 2006, UPAEP (Universidad Popular Autonoma del Estado de Puebla) and OSU (Oklahoma State University) signed a MOU (memorandum of understanding) to develop more than 20 dual master's degree programs. This special partnership has allowed students from Mexico and the United States to study two master degree programs, in two languages, in two…

  11. Exploración del Nuevo Laboratorio Científico de Marte - Duration: 2 minutes, 51 seconds.

    NASA Video Gallery

    Únase a Fernando Abilleira, un analista de trayectoria de la NASA para la Oficina de Exploración de Marte, y conozca las nuevas tecnologías que el nuevo robot Curiosity del Laboratorio Científico d...

  12. Del(18)(q12.2q21.1) syndrome: a case report and clinical review of the literature.

    PubMed

    Imataka, G; Ohwada, Y; Shimura, N; Yoshihara, S; Arisaka, O

    2015-09-01

    The terminal deletion of the long arm of chromosome 18 is relatively common among cytogenetic abnormalities, which occur incidentally in approximately 1 in 40,000 live births. Proximal interstitial deletions of the long arm of chromosome 18 are less frequent. The critical region on chromosome 18 of this syndrome is del(18)(q12.2q21.1) and has recently been recognized as a new clinical entity. We describe a 8-year-old boy with developmental delay, obesity, and epilepsy, with characteristic facial anomalies in whom G-banding chromosome analysis revealed a unique karyotype of 46, XY, del(18)(q12.2q21.1). The patient was diagnosed with interstitial deletion chromosome 18q-syndrome. He received weight control therapy from a medical dietitian. For his epilepsy, he was administered oral carbamazepine at 4 mg/kg/day. At age six, he entered a special needs elementary school. After entering school, he often showed hyperkinesis, and was diagnosed with attention deficit hyperactivity disorder with mild mental retardation. Because patients with only del(18)(q12.2q21.1) have no serious associated malformations, physicians should be aware that even adult patients may have 18q-syndrome. Therefore, if epilepsy occurs in patients with minor facial anomalies, psychomotor retardation, obesity, and the possibility of 18q-syndrome with del(18)(q12.2q21.1) should be kept in mind, and chromosome testing should be performed. PMID:26400529

  13. The Opera del Vocabolario Italiano Database: Full-Text Searching Early Italian Vernacular Sources on the Web.

    ERIC Educational Resources Information Center

    DuPont, Christian

    2001-01-01

    Introduces and describes the functions of the Opera del Vocabolario Italiano (OVI) database, a powerful Web-based, full-text, searchable electronic archive that contains early Italian vernacular texts whose composition may be dated prior to 1375. Examples are drawn from scholars in various disciplines who have employed the OVI in support of their…

  14. Decadal increase in seagrass biomass and temperature at the CARICOMP site in Bocas del Toro, Panama.

    PubMed

    Lpez-Caldern, Jorge M; Guzmn, Hector M; Jcome, Gabriel E; Barnes, Penlope A G

    2013-12-01

    The Caribbean Coastal Marine Productivity Program (CARICOMP) was launched in 1993 to study regional long-term interactions between land and sea, taking standardized measurements of productivity and biomass of mangroves, coral reefs and seagrasses. Since 1999 continuous measurements of seagrass (Thalassia testudinum) parameters as well as environmental data have been recorded in Caribbean Panama. Replicate stations were selected near the Smithsonian Tropical Research Institute in Bocas del Toro. Sediment cores and quadrants were placed there to estimate biomass and productivity, respectively. Mean values for productivity, standing crop, turnover rate, total dry biomass, and Leaf Area Index were 1.74 gDW/m2/d, 66.6 gDW/m2, 2.62%/d, 1481 gDW/m2, and 4.65, respectively. Total dry biomass (shoots, rhizomes and roots) and LAI of T. testudinum increased significantly during the study period. Mean values for total rainfall, Secchi disk depth, sea surface temperature, and salinity were 3498 mm, 8.24 m, 28.79 degrees C, and 32.26 psu, respectively. Sea surface temperature was the only environmental variable with a statistically significant change, increasing from 1999 to 2010. Correlation between sea surface temperature and 7 testudinum parameters (total biomass and LAI) were both positive and significant. Human population has increased dramatically over the last ten years in Bocas del Toro region, increasing pressure (deforestation, runoff, wastewater) over coastal ecosystems (seagrasses, mangroves, coral reefs). Change in the abundance of 7 testudinum may be linked to ocean warming, as a consequence to satisfy plant's metabolic requirements, although other local factors need to be analyzed (reduced grazing and increased eutrophication). A further warming of the ocean could have a negative effect on T. testudinum population, increasing respiratory demands and microbial metabolism. PMID:24432536

  15. A nested large-eddy simulation study of the Ora del Garda wind in the Alps

    NASA Astrophysics Data System (ADS)

    Giovannini, Lorenzo; Laiti, Lavinia; Zardi, Dino

    2015-04-01

    High-resolution numerical simulations performed with the Weather Research and Forecasting (WRF) model are analyzed to investigate the atmospheric boundary layer (ABL) structures associated with the development of a lake-breeze and valley-wind coupled system developing in the southeastern Italian Alps, the so-called "Ora del Garda" wind. Five domains were nested for the simulations: three mesoscale domains, forced by reanalysis data field, are used to drive the finest two domains, in which the large-eddy technique is used, achieving a final horizontal resolution of 80 m. Model results complement an existing dataset composed of a series of measurement flights and surface observations. The flights explored specific valley sections at key locations in the study area, namely over the lake's shore, at half valley and at the end of the valley where the breeze blows. Model results display a good agreement with the experimental dataset. In particular, the surface diurnal cycles of radiation, wind, air temperature and sensible heat flux are satisfactorily reproduced, despite some discrepancies in the timing of thermally-driven circulation onset and offset. The typical structure of the valley ABL, characterized by shallow or even absent mixed layers surmounted by slightly stable layers extending up to the lateral crest level, is also well reproduced in the simulated fields. Moreover, the simulations confirm characteristic local-scale features of the thermally-driven wind field suggested by the analysis of the airborne dataset as well as from previous observations in the area. For example, the model shows the development of inhomogeneities in the cross-valley thermal field, caused by the propagation of the lake breeze and by the different heating between the sidewalls of the valley, as well as the formation of a hydraulic jump in the area where the Ora del Garda flows down into an adjacent valley from an elevated saddle.

  16. Numerical simulations of the Ora del Garda wind in the Alps

    NASA Astrophysics Data System (ADS)

    Giovannini, Lorenzo; Laiti, Lavinia; Zardi, Dino

    2014-05-01

    High-resolution numerical simulations performed with the WRF model are analyzed to investigate the atmospheric boundary layer (ABL) structures associated with the development of a lake-breeze and valley-wind coupled system developing in the southeastern Italian Alps, the so-called "Ora del Garda" wind. Four nested grids are used, achieving a final horizontal resolution of 0.4 km. High-resolution orography and land use datasets are adopted for the domain initialization, while NCEP reanalysis provides initial and boundary conditions for the meteorological fields. Model results complement an existing dataset composed of a series of measurement flights, performed by means of an instrumented motorglider, and surface observations, which is also adopted to validate model results. The flights explored specific valley sections at key locations in the study area, namely over the lake's shore, at half valley and at the end of the valley where the breeze blows. Air pressure, temperature and relative humidity measurements were recorded. Model results display a good agreement with the experimental dataset. In particular, the surface diurnal cycles of radiation, wind and air temperature are satisfactorily reproduced, despite some discrepancies in the timing of thermally-driven circulation onset and offset. The typical structure of the valley ABL, characterized by shallow or even absent mixed layers surmounted by slightly stable layers extending up to the lateral crest level, is also qualitatively well reproduced in the simulated fields. Moreover, the simulations confirm characteristic local-scale features of the thermally-driven wind field suggested by the analysis of the airborne dataset as well as from previous observations in the area. For example, the model shows the development of a well-defined lake breeze front in the lake's shoreline area, as well as the formation of a hydraulic jump structure in the area where the Ora del Garda flows down into an adjacent valley from an elevated saddle.

  17. The del22q11.2 candidate gene Tbx1 regulates branchiomeric myogenesis.

    TOXLINE Toxicology Bibliographic Information

    Kelly RG; Jerome-Majewska LA; Papaioannou VE

    2004-11-15

    Formation and remodeling of the pharyngeal arches play central roles in craniofacial development. TBX1, encoding a T-box-containing transcription factor, is the major candidate gene for del22q11.2 (DiGeorge or velo-cardio-facial) syndrome, characterized by craniofacial defects, thymic hypoplasia, cardiovascular anomalies, velopharyngeal insufficiency and skeletal muscle hypotonia. Tbx1 is expressed in pharyngeal mesoderm, which gives rise to branchiomeric skeletal muscles of the head and neck. Although the genetic control of craniofacial muscle development is known to involve pathways distinct from those operational in the trunk, the regulation of branchiomeric myogenesis has remained enigmatic. Here we show that branchiomeric muscle development is severely perturbed in Tbx1 mutant mice. In the absence of Tbx1, the myogenic determination genes Myf5 and MyoD fail to be normally activated in pharyngeal mesoderm. Unspecified precursor cells expressing genes encoding the transcriptional repressors Capsulin and MyoR are present in the mandibular arch of Tbx1 mutant embryos. Sporadic activation of Myf5 and MyoD in these precursor cells results in the random presence or absence of hypoplastic mandibular arch-derived muscles at later developmental stages. Tbx1 is also required for normal expression of Tlx1 and Fgf10 in pharyngeal mesoderm, in addition to correct neural crest cell patterning in the mandibular arch. Tbx1 therefore regulates the onset of branchiomeric myogenesis and controls normal mandibular arch development, including robust transcriptional activation of myogenic determination genes. While no abnormalities in branchiomeric myogenesis were detected in Tbx1(+/-) mice, reduced TBX1 levels may contribute to pharyngeal hypotonia in del22q11.2 patients.

  18. Antibacterial Activity, Antioxidant Effect and Chemical Composition of Propolis from the Regin del Maule, Central Chile.

    PubMed

    Nina, Nlida; Quispe, Cristina; Jimnez-Aspee, Felipe; Theoduloz, Cristina; Feresn, Gabriela Egly; Lima, Beatriz; Leiva, Elba; Schmeda-Hirschmann, Guillermo

    2015-01-01

    Propolis is commercialized in Chile as an antimicrobial agent. It is obtained mainly from central and southern Chile, but is used for the same purposes regardless of its origin. To compare the antimicrobial effect, the total phenolic (TP), the total flavonoid (TF) content and the phenolic composition, 19 samples were collected in the main production centers in the Regin del Maule, Chile. Samples were extracted with MeOH and assessed for antimicrobial activity against Gram (+) and Gram (-) bacteria. TP and TF content, antioxidant activity by the DPPH, FRAP and TEAC methods were also determined. Sample composition was assessed by HPLD-DAD-ESI-MS/MS. Differential compounds in the samples were isolated and characterized. The antimicrobial effect of the samples showed MICs ranging from 31.5 to > 1000 g/mL. Propolis from the central valley was more effective as antibacterial than those from the coastal area or Andean slopes. The samples considered of interest (MIC ? 62.5 g/mL) showed effect on Escherichia coli, Pseudomonas sp., Yersinia enterocolitica and Salmonella enteritidis. Two new diarylheptanoids, a diterpene, the flavonoids pinocembrin and chrysin were isolated and elucidated by spectroscopic and spectrometric means. Some 29 compounds were dereplicated by HPLC-MS and tentatively identified, including nine flavones/flavonol derivatives, one flavanone, eight dihydroflavonols and nine phenyl-propanoids. Propolis from the Regin del Maule showed large variation in antimicrobial effect, antioxidant activity and composition. So far the presence of diarylheptanoids in samples from the coastal area of central Chile can be considered as a marker of a new type of propolis. PMID:26457694

  19. Vesicularity variation to pyroclasts from silicic eruptions at Laguna del Maule volcanic complex, Chile

    NASA Astrophysics Data System (ADS)

    Wright, H. M. N.; Fierstein, J.; Amigo, A.; Miranda, J.

    2014-12-01

    Crystal-poor rhyodacitic to rhyolitic volcanic eruptions at Laguna del Maule volcanic complex, Chile have produced an astonishing range of textural variation to pyroclasts. Here, we focus on eruptive deposits from two Quaternary eruptions from vents on the northwestern side of the Laguna del Maule basin: the rhyolite of Loma de Los Espejos and the rhyodacite of Laguna Sin Puerto. Clasts in the pyroclastic fall and pyroclastic flow deposits from the rhyolite of Loma de Los Espejos range from dense, non-vesicular (obsidian) to highly vesicular, frothy (coarsely vesicular reticulite); where vesicularity varies from <1% to >90%. Bulk compositions range from 75.6-76.7 wt.% SiO2. The highest vesicularity clasts are found in early fall deposits and widely dispersed pyroclastic flow deposits; the frothy carapace to lava flows is similarly highly vesicular. Pyroclastic deposits also contain tube pumice, and macroscopically folded, finely vesicular, breadcrusted, and heterogeneously vesiculated textures. We speculate that preservation of the highest vesicularities requires relatively low decompression rates or open system degassing such that relaxation times were sufficient to allow extensive vesiculation. Such an inference is in apparent contradiction to documentation of Plinian dispersal to the eruption. Clasts in the pyroclastic fall deposit of the rhyodacite (68-72 wt.% SiO2) of Laguna Sin Puerto are finely vesicular, with vesicularity modes at ~50% and ~68% corresponding to gray and white pumice colors, respectively. Some clasts are banded in color (and vesicularity). All clasts were fragmented into highly angular particles, with subplanar to slightly concave exterior surfaces (average Wadell Roundness of clast margins between 0.32 and 0.39), indicating brittle fragmentation. In contrast to Loma de Los Espejos, high bubble number densities to Laguna Sin Puerto rhyodacite imply high decompression rates.

  20. The Neandertals of northeastern Iberia: new remains from the Cova del Gegant (Sitges, Barcelona).

    PubMed

    Quam, Rolf; Sanz, Montserrat; Daura, Joan; Robson Brown, Kate; García-González, Rebeca; Rodríguez, Laura; Dawson, Heidi; Rodríguez, Rosa Flor; Gómez, Sandra; Villaescusa, Lucía; Rubio, Ángel; Yagüe, Almudena; Ortega Martínez, María Cruz; Fullola, Josep Maria; Zilhão, João; Arsuaga, Juan Luis

    2015-04-01

    The present study describes a new juvenile hominin mandible and teeth and a new juvenile humerus from level V of the GP2 gallery of Cova del Gegant (Spain). The mandible (Gegant-5) preserves a portion of the right mandibular corpus from the M1 distally to the socket for the dc mesially, and the age at death is estimated as 4.5-5.0 years. Gegant-5 shows a single mental foramen located under the dm1/dm2 interdental septum, a relatively posterior placement compared with recent hominins of a similar developmental age. The mental foramen in Gegant-5 is also placed within the lower half of the mandibular corpus, as in the previously described late adolescent/adult mandible (Gegant-1) from this same Middle Paleolithic site. The Gegant-5 canine shows pronounced marginal ridges, a distal accessory ridge, and a pronounced distolingual tubercle. The P3 shows a lingually-displaced protoconid cusp tip and a distal accessory ridge. The P4 shows a slightly asymmetrical crown outline, a continuous transverse crest, a mesially placed metaconid cusp tip, a slight distal accessory ridge, and an accessory lingual cusp. The M1 shows a Y5 pattern of cusp contact and a well-developed and deep anterior fovea bounded posteriorly by a continuous midtrigonid crest. Gegant-4 is the distal portion of a left humerus from a juvenile estimated to be between 5 and 7 years old at death. The specimen shows thick cortical bone. Although fragmentary, the constellation of morphological and metric features indicates Neandertal affinities for these specimens. Their spatial proximity at the site and similar ages at death suggest these remains may represent a single individual. The addition of these new specimens brings the total number of Neandertal remains from the Cova del Gegant to five, and this site documents the clearest evidence for Neandertal fossils associated with Middle Paleolithic stone tools in this region of the Iberian Peninsula. PMID:25766902

  1. Association between Inflammatory Infiltrates and Isolated Monosomy 22/del(22q) in Meningiomas

    PubMed Central

    Domingues, Patrcia Henriques; Teodsio, Cristina; Otero, lvaro; Sousa, Pablo; Ortiz, Javier; Macias, Mara del Carmen Garca; Gonalves, Jess Mara; Nieto, Ana Beln; Lopes, Mara Celeste; de Oliveira, Catarina

    2013-01-01

    Meningiomas contain highly variable levels of infiltrating tissue macrophages (TiMa) and other immune cells. In this study we investigated the potential association between the number and immunophenotype of inflammatory and other immune cells infiltrating the tumor as evaluated by multiparameter flow cytometry, and the clinico-biological, cytogenetic and gene expression profile (GEP) of 75 meningioma patients. Overall, our results showed a close association between the amount and cellular composition of the inflammatory and other immune cell infiltrates and the cytogenetic profile of the tumors. Notably, tumors with isolated monosomy 22/del(22q) showed greater numbers of TiMa, NK cells and (recently)-activated CD69+ lymphocytes versus meningiomas with diploid and complex karyotypes. In addition, in the former cytogenetic subgroup of meningiomas, tumor-infiltrating TiMa also showed a more activated and functionally mature phenotype, as reflected by a greater fraction of CD69+, CD63+, CD16+ and CD33+ cells. GEP at the mRNA level showed a unique GEP among meningiomas with an isolated monosomy 22/del(22q) versus all other cases, which consisted of increased expression of genes involved in inflammatory/immune response, associated with an M1 TiMa phenotype. Altogether, these results suggest that loss of expression of specific genes coded in chromosome 22 (e.g. MIF) is closely associated with an increased homing and potentially also anti-tumoral effect of TiMa, which could contribute to explain the better outcome of this specific good-prognosis cytogenetic subgroup of meningiomas. PMID:24098347

  2. Molecular epidemiology and genetic diversity of hepatitis B virus in Mar del Plata city, Argentina.

    PubMed

    Barbini, Luciana; Elizalde, Mercedes; Torres, Carolina; Campos, Rodolfo

    2013-10-01

    The aim of this work was to describe the current molecular epidemiology and genetic diversity of HBV in Mar del Plata, an important Argentinean touristic city. The phylogenetic analysis of 29 HBV DNA positive serum samples showed that F1b was the predominant subgenotype (sgt, 62.1%), followed by sgt A2 (13.8%) and sgt F4, gt D and gt G (6.9% each). Among anti-HBc IgM positive samples, 75.0% were sgt F1b, followed by sgt F4 (12.5%), sgt A2 (6.25%) and sgt D (6.25%). Three recombinant full length genomes were found: two G/F1b (some of the first gt G detected in Argentina) and one F4/D2. The circulation of clinical important mutations in the city was described. Mutations at the HBsAg were detected in 34.5% of the analyzed samples, associated with laboratory diagnosis and antiviral treatment failures, immune escape and hepatocellular carcinoma. Most of the samples presented wild type BCP/PC sequences. Coalescence analysis for the most prevalent sgt F1b estimated that the diversification mainly occured during mid '90s and the tMRCA was estimated in 1987. Finally, the high presence of the autochthonous sgt F1b, associated with the anti-HBc IgM positive infection and its present-day diversification process, shows the strong impact of internal human migratory movements into the current population of Mar del Plata. PMID:23871776

  3. Bryophyte-Cyanobacteria Associations during Primary Succession in Recently Deglaciated Areas of Tierra del Fuego (Chile)

    PubMed Central

    Arróniz-Crespo, María; Pérez-Ortega, Sergio; De los Ríos, Asunción; Green, T. G. Allan; Ochoa-Hueso, Raúl; Casermeiro, Miguel Ángel; de la Cruz, María Teresa; Pintado, Ana; Palacios, David; Rozzi, Ricardo; Tysklind, Niklas; Sancho, Leopoldo G.

    2014-01-01

    Bryophyte establishment represents a positive feedback process that enhances soil development in newly exposed terrain. Further, biological nitrogen (N) fixation by cyanobacteria in association with mosses can be an important supply of N to terrestrial ecosystems, however the role of these associations during post-glacial primary succession is not yet fully understood. Here, we analyzed chronosequences in front of two receding glaciers with contrasting climatic conditions (wetter vs drier) at Cordillera Darwin (Tierra del Fuego) and found that most mosses had the capacity to support an epiphytic flora of cyanobacteria and exhibited high rates of N2 fixation. Pioneer moss-cyanobacteria associations showed the highest N2 fixation rates (4.60 and 4.96 µg N g−1 bryo. d−1) very early after glacier retreat (4 and 7 years) which may help accelerate soil development under wetter conditions. In drier climate, N2 fixation on bryophyte-cyanobacteria associations was also high (0.94 and 1.42 µg N g−1 bryo. d−1) but peaked at intermediate-aged sites (26 and 66 years). N2 fixation capacity on bryophytes was primarily driven by epiphytic cyanobacteria abundance rather than community composition. Most liverworts showed low colonization and N2 fixation rates, and mosses did not exhibit consistent differences across life forms and habitat (saxicolous vs terricolous). We also found a clear relationship between cyanobacteria genera and the stages of ecological succession, but no relationship was found with host species identity. Glacier forelands in Tierra del Fuego show fast rates of soil transformation which imply large quantities of N inputs. Our results highlight the potential contribution of bryophyte-cyanobacteria associations to N accumulation during post-glacial primary succession and further describe the factors that drive N2-fixation rates in post-glacial areas with very low N deposition. PMID:24819926

  4. Bryophyte-cyanobacteria associations during primary succession in recently Deglaciated areas of Tierra del Fuego (Chile).

    PubMed

    Arróniz-Crespo, María; Pérez-Ortega, Sergio; De Los Ríos, Asunción; Green, T G Allan; Ochoa-Hueso, Raúl; Casermeiro, Miguel Ángel; de la Cruz, María Teresa; Pintado, Ana; Palacios, David; Rozzi, Ricardo; Tysklind, Niklas; Sancho, Leopoldo G

    2014-01-01

    Bryophyte establishment represents a positive feedback process that enhances soil development in newly exposed terrain. Further, biological nitrogen (N) fixation by cyanobacteria in association with mosses can be an important supply of N to terrestrial ecosystems, however the role of these associations during post-glacial primary succession is not yet fully understood. Here, we analyzed chronosequences in front of two receding glaciers with contrasting climatic conditions (wetter vs drier) at Cordillera Darwin (Tierra del Fuego) and found that most mosses had the capacity to support an epiphytic flora of cyanobacteria and exhibited high rates of N2 fixation. Pioneer moss-cyanobacteria associations showed the highest N2 fixation rates (4.60 and 4.96 µg N g-1 bryo. d-1) very early after glacier retreat (4 and 7 years) which may help accelerate soil development under wetter conditions. In drier climate, N2 fixation on bryophyte-cyanobacteria associations was also high (0.94 and 1.42 µg N g-1 bryo. d-1) but peaked at intermediate-aged sites (26 and 66 years). N2 fixation capacity on bryophytes was primarily driven by epiphytic cyanobacteria abundance rather than community composition. Most liverworts showed low colonization and N2 fixation rates, and mosses did not exhibit consistent differences across life forms and habitat (saxicolous vs terricolous). We also found a clear relationship between cyanobacteria genera and the stages of ecological succession, but no relationship was found with host species identity. Glacier forelands in Tierra del Fuego show fast rates of soil transformation which imply large quantities of N inputs. Our results highlight the potential contribution of bryophyte-cyanobacteria associations to N accumulation during post-glacial primary succession and further describe the factors that drive N2-fixation rates in post-glacial areas with very low N deposition. PMID:24819926

  5. Reciprocal relation between GADD153 and Del-1 in regulation of salivary gland inflammation in Sjgren syndrome.

    PubMed

    Baban, Babak; Liu, Jun Yao; Abdelsayed, Rafik; Mozaffari, Mahmood S

    2013-12-01

    Endoplasmic reticulum (ER) stress response is a pivotal regulator of inflammation and cell death. An integral component of ER stress-induced apoptosis is expression of growth arrest- and DNA damage-inducible protein 153 (GADD153). Further, ER stress response is implicated in leukocyte adhesion and recent studies have discovered endogenous inhibitors of leukocyte adhesion including the developmental endothelial locus-1 (Del-1). Accordingly, we tested the hypothesis that Sjgren's syndrome (SS) is associated with increased salivary gland expression of GADD153 and increased leukocyte infiltration in association with decreased Del-1 thereby contributing to inflammation and cell death. We utilized the non-obese diabetic (NOD) mice, a model of SS-like disease, in association with immunostaining and flow cytometry-based studies. Salivary glands of 14-week-old NOD mice displayed a) increased GADD153 expression, b) marked reduction in Del-1, c) inflammatory cell infiltrates including CD3+ T and CD19+ B lymphocytes as well as M1 and M2 macrophages and d) increased pro-inflammatory interleukin (IL)-17 but reduced anti-inflammatory cytokine, IL-10. These changes were accompanied with disruption of mitochondrial membrane potential and significant increase in apoptosis and necrosis of salivary gland cells of NOD than control mice. Our collective observations suggested that GADD153 directly and/or indirectly through downregulation of Del-1 contributes importantly to salivary gland inflammation and cell death. To establish the relevance of GADD153 and Del-1 for the human condition, lower lip biopsy samples of non-SS subjects and those with a diagnosis of SS were subjected to immunohistochemistry. The results show intense GADD153 immunostaining but marked reduction in Del-1 expression in biopsy samples of SS compared to non-SS subjects. Collectively, the results indicate that GADD153 regulates inflammation and cell death in salivary gland in SS. Further, Del-1 expression likely provides a mechanistic link between increased GADD153 and leukocyte infiltration and accompanying inflammation of salivary gland tissue in this condition. PMID:24060278

  6. Glacial geomorphology of the Torres del Paine region (southern Patagonia): Implications for glaciation, deglaciation and paleolake history

    NASA Astrophysics Data System (ADS)

    Garca, Juan-Luis; Hall, Brenda L.; Kaplan, Michael R.; Vega, Rodrigo M.; Strelin, Jorge A.

    2014-01-01

    The processes affecting paleoclimate variability and Pleistocene glacial landscape development in the southern mid-latitudes remain poorly understood, in part because of the scarcity of comprehensive, well-studied records. Glacial landforms are invaluable for reconstructing past ice-sheet, climate, and associated environmental changes along the southern Andes, but there are significant spatial and temporal gaps in existing data. In this paper, we present new geomorphic and sedimentologic analyses, including surficial maps, for the Torres del Paine region (51S, 73W), southern South America. Our findings provide a new framework for understanding changes in the regional glacier history and Pleistocene landscape development. Glacial extent during the local last glacial maximum (LGM) remains unknown but new chronological data supported by geomorphic evidence afford evidence for a larger ice sheet at Torres del Paine than previously assumed. Deglaciation from the local LGM was underway by 17,400 200 (1?) cal. yr. BP. As opposed to previous suggestions, we have found that most of the moraines fringing the lakes in the Torres del Paine national park were deposited during a late-glacial expansion that occurred between 14,100 and 12,500 cal. yr. BP. Late-glacial advances also have been documented recently for the ltima Esperanza and Lago Argentino basins to the south and north of Torres del Paine, respectively, suggesting an overall regional ice response to a climate signal. The Tehuelche paleolake accompanied each of the ice-sheet fluctuations in Torres del Paine. New data document at least three main phases of this paleolake, which drained eastward to the Atlantic Ocean, while the Andes gaps were blocked with ice. During the late phase of glacial lake formation, when water levels reached 125-155 m a.s.l., the lake likely merged with paleolake Consuelo in the ltima Esperanza area at the end of the last glaciation. Lake Tehuelche in Torres del Paine had drained into the Pacific Ocean by the late-glacial period, suggesting that ice southwest of Torres del Paine may have retreated back into the mountains by this time.

  7. High Host Specificity in Encarsia diaspidicola (Hymenoptera: Aphelinidae), a Biological Control Candidate Against the White Peach Scale in Hawaii

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pre-introductory host specificity tests were performed with Encarsia diaspidicola, a biological control candidate against the invasive white peach scale, Pseudaulacaspis pentagona. False oleander scale, P. cockerelli, coconut scale, Aspidiotus destructor, cycad scale, Aulacaspis yasumatsui, greenh...

  8. Prevalencia y tamizaje del Trastorno por Déficit de Atención con Hiperactividad en Costa Rica

    PubMed Central

    Weiss, Nicholas T.; Schuler, Jovita; Monge, Silvia; McGough, James J.; Chavira, Denise; Bagnarello, Monica; Herrera, Luis Diego; Mathews, Carol A.

    2015-01-01

    Resumen La investigación tuvo como propósito estimar la prevalencia del Trastorno por Déficit de Atención con Hiperactividad (TDAH) en Costa Rica y determinar si la versión en español del cuestionario Swanson Nolan and Pelham Scale IV (SNAP-IV) es un instrumento de tamizaje útil en una población de niños y niñas escolares costarricenses. El instrumento fue entregado a padres y maestros de 425 niños entre 5 y 13 años de edad (promedio = 8.8). Todos fueron evaluados con el instrumento Swanson, Kotkin, Agler, M-Flynn and Pelham Scale (SKAMP). Su diagnóstico fue confirmado con una entrevista clínica. La sensibilidad y la especificidad del SNAP-IV fueron evaluadas como predictores de criterios de diagnóstico según el DSM-IV. La prevalencia puntual en la muestra del TDAH fue del 5%. El tamizaje más preciso lo hizo el SNAP-IV completado por el maestro en un corte de 20%, con una sensibilidad de 96% y una especificidad de un 82%. La sensibilidad de los instrumentos completados por los padres fue más baja que aquella de los maestros. El SNAP-IV completado por las maestras con un corte aislando el 20% de los mayores puntajes categorizó correctamente a un 87% de los sujetos. PMID:22432094

  9. Targeted re-sequencing analysis of 25 genes commonly mutated in myeloid disorders in del(5q) myelodysplastic syndromes

    PubMed Central

    Fernandez-Mercado, Marta; Burns, Adam; Pellagatti, Andrea; Giagounidis, Aristoteles; Germing, Ulrich; Agirre, Xabier; Prosper, Felipe; Aul, Carlo; Killick, Sally; Wainscoat, James S.; Schuh, Anna; Boultwood, Jacqueline

    2013-01-01

    Interstitial deletion of chromosome 5q is the most common chromosomal abnormality in myelodysplastic syndromes. The catalogue of genes involved in the molecular pathogenesis of myelodysplastic syndromes is rapidly expanding and next-generation sequencing technology allows detection of these mutations at great depth. Here we describe the design, validation and application of a targeted next-generation sequencing approach to simultaneously screen 25 genes mutated in myeloid malignancies. We used this method alongside single nucleotide polymorphism-array technology to characterize the mutational and cytogenetic profile of 43 cases of early or advanced del(5q) myelodysplastic syndromes. A total of 29 mutations were detected in our cohort. Overall, 45% of early and 66.7% of advanced cases had at least one mutation. Genes with the highest mutation frequency among advanced cases were TP53 and ASXL1 (25% of patients each). These showed a lower mutation frequency in cases of 5q- syndrome (4.5% and 13.6%, respectively), suggesting a role in disease progression in del(5q) myelodysplastic syndromes. Fifty-two percent of mutations identified were in genes involved in epigenetic regulation (ASXL1, TET2, DNMT3A and JAK2). Six mutations had allele frequencies <20%, likely below the detection limit of traditional sequencing methods. Genomic array data showed that cases of advanced del(5q) myelodysplastic syndrome had a complex background of cytogenetic aberrations, often encompassing genes involved in myeloid disorders. Our study is the first to investigate the molecular pathogenesis of early and advanced del(5q) myelodysplastic syndromes using next-generation sequencing technology on a large panel of genes frequently mutated in myeloid malignancies, further illuminating the molecular landscape of del(5q) myelodysplastic syndromes. PMID:23831921

  10. Targeted re-sequencing analysis of 25 genes commonly mutated in myeloid disorders in del(5q) myelodysplastic syndromes.

    PubMed

    Fernandez-Mercado, Marta; Burns, Adam; Pellagatti, Andrea; Giagounidis, Aristoteles; Germing, Ulrich; Agirre, Xabier; Prosper, Felipe; Aul, Carlo; Killick, Sally; Wainscoat, James S; Schuh, Anna; Boultwood, Jacqueline

    2013-12-01

    Interstitial deletion of chromosome 5q is the most common chromosomal abnormality in myelodysplastic syndromes. The catalogue of genes involved in the molecular pathogenesis of myelodysplastic syndromes is rapidly expanding and next-generation sequencing technology allows detection of these mutations at great depth. Here we describe the design, validation and application of a targeted next-generation sequencing approach to simultaneously screen 25 genes mutated in myeloid malignancies. We used this method alongside single nucleotide polymorphism-array technology to characterize the mutational and cytogenetic profile of 43 cases of early or advanced del(5q) myelodysplastic syndromes. A total of 29 mutations were detected in our cohort. Overall, 45% of early and 66.7% of advanced cases had at least one mutation. Genes with the highest mutation frequency among advanced cases were TP53 and ASXL1 (25% of patients each). These showed a lower mutation frequency in cases of 5q- syndrome (4.5% and 13.6%, respectively), suggesting a role in disease progression in del(5q) myelodysplastic syndromes. Fifty-two percent of mutations identified were in genes involved in epigenetic regulation (ASXL1, TET2, DNMT3A and JAK2). Six mutations had allele frequencies <20%, likely below the detection limit of traditional sequencing methods. Genomic array data showed that cases of advanced del(5q) myelodysplastic syndrome had a complex background of cytogenetic aberrations, often encompassing genes involved in myeloid disorders. Our study is the first to investigate the molecular pathogenesis of early and advanced del(5q) myelodysplastic syndromes using next-generation sequencing technology on a large panel of genes frequently mutated in myeloid malignancies, further illuminating the molecular landscape of del(5q) myelodysplastic syndromes. PMID:23831921

  11. Effect of human activated NRAS on replication of delNS1 H5N1 influenza virus in MDCK cells

    PubMed Central

    2011-01-01

    Background RAS, coded by ras proto-oncogenes, played an important role in signal transmission to regulate cell growth and differentiation. Host activation of RAS was significant for IFN-sensitive vaccinia virus (delE3L) or attenuate influenza virus in unallowable cells. Results Huamn NRAS gene was activated by mutating in codon 61. Then the activation of NRAS was detected by western blot in MDCK cells. The delNS1 H5N1 influenza virus with deletion of NS1 eIF4GI binding domain was weak multiplication in MDCK cells. And the replication of delNS1 virus and expression of IFN-beta and IRF-3 were detected by Real-time PCR in MDCK cells infected with delNS1 virus. It was found that the delNS1 virus had a significant increase in MDCK cells when the NRAS was activated, and yet, expression of IRF-3 and IFN-beta were restrained. Conclusions The study demonstrated that activated NRAS played an important part for delNS1 virus replication in MDCK cells. Activated NRAS might be down-regulating the expression of antiviral cellular factors in delNS1 virus infected cells. PMID:21595878

  12. La utilizacion de los mapas conceptuales en la ensenanza de biologia y su efecto sobre el dominio del proceso de fotosintesis en los estudiantes universitarios

    NASA Astrophysics Data System (ADS)

    Gonzalez Rivera, Maria M.

    Se investigo el efecto de los mapas conceptuales sobre el dominio del proceso de fotosintesis en estudiantes universitarios. La investigacion utilizo dos estrategias: mapas conceptuales individuales y mapas conceptuales colaborativos, con el fin de investigar si existen diferencias significativas en el dominio del proceso de fotosintesis. El analisis de los datos incluyo aspectos cualitativos y cuantitativos. Se desprende del estudio que el 80% de los estudiantes describen la utilizacion de los mapas conceptuales como una experiencia beneficiosa. El 70% de los estudiantes expreso que los mapas conceptuales son utiles en el aprendizaje del proceso de fotosintesis y el 61% indico que facilitan la comprension de los conceptos. Los hallazgos mas importantes del analisis cuantitativo indican que los estudiantes que utilizaron los mapas conceptuales mejoraron significativamente su desempeno en la posprueba global. Se utilizo la prueba Mann-Whitney para investigar si existian diferencias significativas en la posprueba y preprueba global, el valor de W = 1945.0, para un valor p de 0.00, lo cual establece diferencias significativas. Para determinar si existian diferencias significativas entre la posprueba y preprueba del grupo individual, se realizo la prueba nuevamente. El valor de W correspondio a 490.5, que es significativo, con un valor p de 0.00. Se concluye que existen diferencias significativas entre la ejecucion de la posprueba y preprueba del grupo individual. Los datos proveen suficiente evidencia para sostener que los estudiantes que utilizaron la estrategia de mapas conceptuales individuales mejoraron el dominio del proceso de fotosintesis significativamente. Se realizo nuevamente la prueba para los resultados de posprueba y preprueba del grupo colaborativo. El valor de W correspondio a 446 con un valor p de 0.00. Se concluyo que existen diferencias significativas entre la ejecucion de la posprueba y preprueba del grupo colaborativo. Finalmente, se efectuo una prueba Mann-Whitney comparando la posprueba de ambos grupos. El valor de W fue de a 777, para un valor p de 0.2782. Aunque las puntuaciones de las pospruebas del grupo colaborativo fueron mayores que las puntuaciones de la posprueba del grupo individual, la diferencia no fue estadisticamente significativa.

  13. Spatial variability of cv Tempranillo phenology within the Ribera del Duero DO (Spain) related to soil type and plot characteristics

    NASA Astrophysics Data System (ADS)

    Ramos, Maria C.; Jones, Gregory V.; Yuste, Jess

    2014-05-01

    Vineyards in the Ribera del Duero area (Spain) date back to the roman time, with significant fluctuations throughout the centuries, reaching consolidation and stable production in the 10th and 11th centuries. The present Ribera del Duero Designation of Origen (DO) started in 1980 and has increased from less than 6,000 ha planted to more than 21,000 ha planted by 2010 and becoming one the top regions for wine production in Spain with success in the global wine market. Vineyards of Ribera del Duero are found along 115 km of the Duero River. Within this distance, differences in elevation and topography produce differences in climate, which provide the means by which a zonification for vineyard management can be assessed. In this work, the differences in phenological growth characteristics for the Tempranillo variety were analyzed within the Ribera del Duero DO and related to soil and plot characteristics. Phenology dates, referenced to different Baggiolini classification stages, were evaluated in 20 control plots distributed throughout the Ribera del Duero DO during the period 2004-2012. These years presented different climatic characteristics, which were analyzed from daily temperature and precipitation series recorded from 1980 to 2012 at five stations located along the Duero River. Most vineyards were cultivated under rainfed conditions. In order to evaluate the spatial variability in phenology, climate and soils, the plots were classified using a hierarchical cluster multivariate analysis. Parameters such as the elevation, the distance to river and the soil type were taken into account in the classification. According to the WRB classification (2006) the main soil types in the area are Calcaric Cambisols, Eutric Cambisols, Calcic Luvisol, Calcaric fluvisols, Eutric fluvisols and in less proportion Lithic Leptosols and Calcaric Rogosols. Four different groups were observed, with differences in elevation, distance to the Duero River and the soil type. The differences in phenology among groups started in stage G and were observed until the end of the growing cycle. Despite the high variability driven by the year to year in climate characteristics, it was possible to define the soil and plot characteristics that favour advanced phenology within the Ribera del Duero DO.

  14. [Teoria della Mente e funzionamento sociale nella schizofrenia: correlazione con anomalie del linguaggio figurato, sintomatologia clinica e intelligenza generale].

    PubMed

    Piovan, Cristiano; Gava, Laura; Campeol, Mara

    2016-01-01

    RIASSUNTO. Scopo. Negli ultimi decenni gli studi hanno mostrato come la Teoria della Mente (ToM) non sia un processo unitario, ma un sistema che include aspetti cognitivi e affettivi. Nell'ambito dei domini che definiscono la social cognition, la ToM rappresenta il miglior predittore dello scarso funzionamento sociale nella schizofrenia. Lo scopo del presente lavoro è stato di esaminare la competenza di un gruppo di pazienti ambulatoriali affetti da schizofrenia in compiti di ToM, di riconoscimento di aspetti metaforici e idiomatici del linguaggio, in una prova di rispetto di regole conversazionali e di indagarne la relazione con il funzionamento sociale. Metodi. Sono stati reclutati 30 pazienti ambulatoriali con diagnosi di schizofrenia e 24 controlli sani. Sono stati somministrati il TIB per il calcolo del QI premorboso, la PANSS, il Theory of Mind Picture Sequencing Task, un test di comprensione di metafore e idiomi e un test conversazionale. Il funzionamento sociale è stato valutato con la PSP. Risultati. Non vi era differenza significativa tra i valori medi del QI premorboso del gruppo dei pazienti e dei controlli. Ai test di ToM e di competenza pragmatica, la differenza tra i gruppi è risultata altamente significativa, con i pazienti che hanno eseguito le prove in modo peggiore. È emersa una correlazione tra la comprensione di metafore e idiomi e le false credenze di secondo ordine. La PSP è risultata correlata con la PANSS e con la quota di ToM cognitiva, ma non con la ToM affettiva. Conclusioni. I risultati hanno mostrato che i soggetti affetti da schizofrenia, in condizione clinica di stabilizzazione, hanno evidenti difficoltà nelle prove di ToM e di comprensione del linguaggio figurato. Nel nostro modello teorico, la correlazione evidenziata tra la ToM cognitiva, i deficit pragmatici, lo stato clinico e il livello di funzionamento sociale suggerisce l'utilità di interventi riabilitativi di recupero delle funzioni metacognitive e delle abilità pragmatiche, al fine di ridurre la disabilità sociale nella schizofrenia. PMID:27030346

  15. Analysis of strong rainfall events at Cividale del Friuli (Northeastern Italy) from 1920 to 2007

    NASA Astrophysics Data System (ADS)

    Colucci, R. R.; Cucchi, F.; Stravisi, F.; Zini, L.

    2009-04-01

    Strong rainfall events have been selected from a detailed statistical investigation of the rainfall regime recorded at Cividale del Friuli (which is a town situated in the piedmont area of the Italian Prealpi Giulie), by applying suitable thresholds on the set of monthly and daily rainfall. On the basis of these data the following aspects as been explored: Time evolution of the location of the most rainy month, for each year, in the meteorological seasons; Return period, in years, of the maximum monthly precipitation, for each year; Return period, in years, of the maximum daily precipitation, for each year; Return period, in years, of the maximum precipitation during two consecutive days, for each year; Trend of the series of rainy days above a given thresholds, for different thresholds; Long term evolution of the infra-annual precipitation by averaging four 21-years cycles of rainfall for the periods 1924-44, 1945-65, 1966-86, 1987-2007; Comparison of the above rainfall series with that of Trieste which is a coastal town situated at the north-eastern most part of the Adriatic sea, about 70 km far from Cividale del Friuli (which is usually much more rainy than Trieste). Unlike the almost constant linear trend for Trieste, that of Cividale del Friuli is markedly decreasing in the whole period 1920-2007. This phenomenon is ascribed to a recent climatic change in the mesoscale wind regime in which winds from NW-N-NE are more frequent than those zonal. Therefore in the areas more close to the mountains a lesser and lesser quantity of rain is recorded relatively to the areas far enough from the mountains like Trieste. The different behaviour is more evident during spring and summer which are the seasons with the higher occurrence of thunderstorms. Winter period shows a different behaviour between the years before and after 1980; in the last 30 years the decreasing of the differences has been very pronounced. Further conclusions are the following: Total yearly rainfalls show a relevant decrease of about -277mm/100y; The most rainy month is July for the whole interval 1920-2007, but this month undergoes an important climatic change with the tendency to become a typical late summer month; The soul month in which rainfalls has increased is September, on the other hand January, July, October and December do not exhibit significant variations while the remaning months tend to become more dry; Total rainfall on September 1965, that is 708 mm, is quite exceptional and probably such values has a return period much longer than those analyzed in this work; The number of days with strong rainfalls (50-75 mm/d) is constant in the considered interval and this implies the tendency to less frequent but more intense rainfall events; Intense rainfall during two days take placed more frequently from Maj to June and from September to November and 17 cases have been recorded with intensity higher than 150mm/2d; In general, the return period of a rainfall with 100mm/2d is 1.5 years, of one with 150mm/2d is about 5 years and of one over 200mm/2d is beyond 30 years; The maximum rainfall that has taken placed on two consecutive days reached 210.4 mm;

  16. Presence of c.3956delC mutation in familial adenomatous polyposis patients from Brazil

    PubMed Central

    Moreira-Nunes, Caroline Aquino; Alcntara, Diego di Felipe vila; Lima-Jnior, Srgio Figueiredo; Cavallro, Sandro Roberto de Arajo; Rey, Juan Antonio; Pinto, Giovanny Rebouas; de Assumpo, Paulo Pimentel; Burbano, Rommel Rodriguez

    2015-01-01

    AIM: To characterize APC gene mutations and correlate them with patient phenotypes in individuals diagnosed with familial adenomatous polyposis (FAP) in northern Brazil. METHODS: A total of 15 individuals diagnosed with FAP from 5 different families from the north of Brazil were analyzed in this study. In addition to patients with histopathological diagnosis of FAP, family members who had not developed the disease were also tested in order to identify mutations and for possible genetic counseling. All analyzed patients or their guardians signed a consent form approved by the Research Ethics Committee of the Joo de Barros Barreto University Hospital (Belem, Brazil). DNA extracted from the peripheral blood of a member of each of the affected families was subjected to direct sequencing. The proband of each family was sequenced to identify germline mutations using the Ion Torrent platform. To validate the detected mutations, Sanger sequencing was also performed. The samples from all patients were also tested for the identification of mutations by real-time quantitative polymerase chain reaction using the amplification refractory mutation system. RESULTS: Through interviews with relatives and a search of medical records, it was possible to construct genograms for three of the five families included in the study. All 15 patients from the five families with FAP exhibited mutations in the APC gene, and all mutations were detected in exon 15 of the APC gene. In addition to the patients with a histological diagnosis of FAP, family members without disease symptoms showed the mutation in the APC gene. In the present study, we detected two of the three most frequent germline mutations in the literature: the mutation at codon 1309 and the mutation at codon 1061. The presence of c.3956delC mutation was found in all families from this study, and suggests that this mutation was introduced in the population of the State of Par through ancestor immigration (i.e., a de novo mutation that arose in one member belonging to this state from Brazil). CONCLUSION: Regardless of its origin, the c.3956delC mutation is a strong candidate biomarker of this hereditary cancer syndrome in families of northern Brazil. PMID:26309368

  17. Impacts of acid emissions from Nevado del Ruiz volcano, Colombia, on selected terrestrial and aquatic ecosystems

    NASA Astrophysics Data System (ADS)

    Parnell, Roderic A.; Burke, Kelly J.

    1990-07-01

    Emissions of acidic gases and thermal waters from Nevado del Ruiz volcano have recently increased in concert with the November 13, 1985 eruption. This study examines the downwind and downstream effects of these emissions on alpine ecosystems high on the slopes of the volcano (4100 m) and on coffee plantations at lower elevations (< 2000 m) and greater distances from the active vent (> 30 km). Samples of bulk deposition, rain, soils, soil solutions, and streams were collected over a six-month period (January-July, 1987) to examine the impacts of this volcanogenic acidity. Bulk deposition falling on the higher slopes of the volcano is usually acidified; however, deposition reaching the distal coffee plantations seldom is acidic. The sources of the acids are hydrogen chloride and sulfur dioxide in the plume of the volcano. Although sulfur dioxide is by far the more abundant gas, hydrogen chloride is most responsible for acidification of rain falling on the slopes of the volcano. With distance from the vent, the chloride/sulfate ratio drops exponentially. The only major influence on regional precipitation chemistry in addition to the volcano appears to be land-use-related activities around the coffee plantations. Deposition on these areas is enriched by an order of magnitude in nitrate and base cations, compared to all other stations. Throughfall chemistry in the coffee plantations shows a dramatic response to occasional acid-rain events. A base-leaching process on coffee plant leaves is triggered by acid rain. For each equivalent of hydrogen ion in rain on the leaf surface, over 23 equivalents of potassium ion are leached from the leaf. In spite of this dramatic response by the vegetation, the plantation soils appear relatively unaffected by acidic deposition. In contrast, the alpine soils on the volcano exhibit low pHs, high sulfate and chloride concentrations in soil solutions, and high extractable sulfate concentrations. All of these factors indicate that these soils have undergone significant acid loading. While the deposition of the region is acidified by hydrogen chloride, the streams flowing off the volcano are apparently acidified by sulfuric acid in thermal waters discharging into the streams. The acidity of these streams decreases downstream, while the silica concentrations increase downstream. The composition of stream water is most influenced by thermal-water discharges as well as equilibrium dissolution of amorphous silica glass and non-equilibrium leaching of unweathered ash. The impacts of acid gases and thermal water released from the volcano appear to be restricted to ecosystems on the slope of the volcano. The only impact of Nevado del Ruiz on surrounding coffee plantations appears to be potassium leaching of coffee leaves from occasional acid-deposition events.

  18. Assessing the ecological effects of human impacts on coral reefs in Bocas del Toro, Panama.

    PubMed

    Seemann, Janina; González, Cindy T; Carballo-Bolaños, Rodrigo; Berry, Kathryn; Heiss, Georg A; Struck, Ulrich; Leinfelder, Reinhold R

    2014-03-01

    Environmental and biological reef monitoring was conducted in Almirante Bay (Bahía Almirante) in Bocas del Toro, Panama, to assess impacts from anthropogenic developments. An integrated monitoring investigated how seasonal temperature stress, turbidity, eutrophication and physical impacts threatened reef health and biodiversity throughout the region. Environmental parameters such as total suspended solids [TSS], carbon isotopes (δ(13)C), C/N ratios, chlorophyll a, irradiance, secchi depth, size fractions of the sediments and isotope composition of dissolved inorganic carbon [DIC] of the water were measured throughout the years 2010 and 2011 and were analysed in order to identify different impact sources. Compared to data from Collin et al. (Smithsonian Contributions to the Marine Sciences 38:324-334, 2009) chlorophyll a has doubled at sites close to the city and the port Almirante (from 0.46-0.49 to 0.78-0.97 μg l(-1)) and suspension load increased, visible by a decrease in secchi depth values. Visibility decreased from 9-13 m down to 4 m at the bay inlet Boca del Drago, which is strongly exposed to river run off and dredging for the shipping traffic. Eutrophication and turbidity levels seemed to be the determining factor for the loss of hard coral diversity, most significant at chlorophyll a levels higher than 0.5 μg l(-1) and TSS levels higher than 4.7 mg l(-1). Hard coral cover within the bay has also declined, at some sites down to <10 % with extremely low diversities (7 hard coral species). The hard coral species Porites furcata dominated the reefs in highly impacted areas and showed a strong recovery after bleaching and a higher tolerance to turbidity and eutrophication compared to other hard coral species in the bay. Serious overfishing was detected in the region by a lack of adult and carnivorous fish species, such as grunts, snappers and groupers. Study sites less impacted by anthropogenic activities and/or those with local protection showed a higher hard coral cover and fish abundance; however, an overall loss of hard coral diversity was observed. PMID:24254491

  19. Junctional abnormalities in human airway epithelial cells expressing F508del CFTR.

    PubMed

    Molina, Samuel A; Stauffer, Brandon; Moriarty, Hannah K; Kim, Agnes H; McCarty, Nael A; Koval, Michael

    2015-09-01

    Cystic fibrosis (CF) has a profound impact on airway physiology. Accumulating evidence suggests that intercellular junctions are impaired in CF. We examined changes to CF transmembrane conductance regulator (CFTR) function, tight junctions, and gap junctions in NuLi-1 (CFTR(wt/wt)) and CuFi-5 (CFTR(?F508/?F508)) cells. Cells were studied at air-liquid interface (ALI) and compared with primary human bronchial epithelial cells. On the basis of fluorescent lectin binding, the phenotype of the NuLi-1 and CuFi-5 cells at week 8 resembled that of serous, glycoprotein-rich airway cells. After week 7, CuFi-5 cells possessed 130% of the epithelial Na(+) channel activity and 17% of the CFTR activity of NuLi-1 cells. In both cell types, expression levels of CFTR were comparable to those in primary airway epithelia. Transepithelial resistance of NuLi-1 and CuFi-5 cells stabilized during maturation in ALI culture, with significantly lower transepithelial resistance for CuFi-5 than NuLi-1 cells. We also found that F508del CFTR negatively affects gap junction function in the airway. NuLi-1 and CuFi-5 cells express the connexins Cx43 and Cx26. While both connexins were properly trafficked by NuLi-1 cells, Cx43 was mistrafficked by CuFi-5 cells. Cx43 trafficking was rescued in CuFi-5 cells treated with 4-phenylbutyric acid (4-PBA), as assessed by intracellular dye transfer. 4-PBA-treated CuFi-5 cells also exhibited an increase in forskolin-induced CFTR-mediated currents. The Cx43 trafficking defect was confirmed using IB3-1 cells and found to be corrected by 4-PBA treatment. These data support the use of NuLi-1 and CuFi-5 cells to examine the effects of F508del CFTR expression on tight junction and gap junction function in the context of serous human airway cells. PMID:26115671

  20. Rock Magnetic Properties of Laguna Carmen (Tierra del Fuego, Argentina): Implications for Paleomagnetic Reconstruction

    NASA Astrophysics Data System (ADS)

    Gogorza, C. G.; Orgeira, M. J.; Ponce, F.; Fernndez, M.; Laprida, C.; Coronato, A.

    2013-05-01

    We report preliminary results obtained from a multi-proxy analysis including paleomagnetic and rock-magnetic studies of two sediment cores of Laguna Carmen (5340'60" S 6819'0" W, ~83m asl) in the semiarid steppe in northern Tierra del Fuego island, Southernmost Patagonia, Argentina. Two short cores (115 cm) were sampled using a Livingstone piston corer during the 2011 southern fall. Sediments are massive green clays (115 to 70 cm depth) with irregularly spaced thin sandy strata and lens. Massive yellow clay with thin sandy strata continues up to 30 cm depth; from here up to 10 cm yellow massive clays domain. The topmost 10 cm are mixed yellow and green clays with fine sand. Measurements of intensity and directions of Natural Remanent Magnetization (NRM), magnetic susceptibility, isothermal remanent magnetization, saturation isothermal remanent magnetization (SIRM), back field and anhysteretic remanent magnetization at 100 mT (ARM100mT) were performed and several associated parameters calculated (ARM100mT/k and SIRM/ ARM100mT). Also, as a first estimate of relative magnetic grain-size variations, the median destructive field of the NRM (MDFNRM), was determined. Additionally, we present results of magnetic parameters measured with vibrating sample magnetometer (VSM). The stability of the NRM was analyzed by alternating field demagnetization. The magnetic properties have shown variable values, showing changes in both grain size and concentration of magnetic minerals. It was found that the main carrier of remanence is magnetite with the presence of hematite in very low percentages. This is the first paleomagnetic study performed in lakes located in the northern, semiarid fuegian steppe, where humid-dry cycles have been interpreted all along the Holocene from an aeolian paleosoil sequence (Orgeira et el, 2012). Comparison between paleomagnetic records of Laguna Carmen and results obtained in earlier studies carried out at Laguna Potrok Aike (Gogorza et al., 2012) were performed. References Gogorza, C.S.G., Irurzun, M.A., Sinito, A.M., Lis-Pronovost, A., St-Onge, G., Haberzettl, T., Ohlendorf, C., Kastner, S., Zolitschka, B., 2012. High-resolution paleomagnetic records from Laguna Potrok Aike (Patagonia, Argentina) for the last 16,000 years. Geochemistry Geophysics Geosystems. 13, Q12Z37. Orgeira, M.J., Vsquez, C.A., Coronato, A., Ponce, F., Moreto, A., Osterrieth, M, Egli, R., Onorato, R., 2012. Magnetic properties of Holocene edaphized silty eolian sediments from Tierra del Fuego (Argentina). Revista de la Sociedad Geolgica de Espaa. 25 (1-2), 45-56.

  1. EJERCICIO Y LA DETECCION DEL MAL AGUDO DE MONTAÑA GRAVE

    PubMed Central

    Garófoli, Adrián; Montoya, Paola; Elías, Carlos; Benzo, Roberto

    2012-01-01

    El Mal Agudo de Montaña (MAM) es un conjunto de síntomas inespecíficos padecidos por sujetos que ascienden rápidamente desde baja a alta altura sin adecuada aclimatación. Usualmente es autolimitado, pero las formas graves (edema pulmonar y cerebral) pueden causar la muerte. La hipoxemia exagerada en reposo está relacionada con el desarrollo de MAM pero su valor predictivo es limitado. Dado que el ejercicio en altura se acompaña de mayor hipoxemia y síntomas, postulamos el valor predictivo de un simple test de ejercicio para pronosticar MAM grave. Se estudió el valor predictivo de la saturación de oxígeno en reposo y ejercicio submáximo a 2 700m y 4 300m en 63 sujetos que ascendían al cerro Aconcagua (6 962m). Se consideró desaturación de oxígeno con ejercicio a una disminución >=5% respecto al reposo. Se utilizó la escala de Lake-Louise para establecer la presencia de MAM grave. 6 sujetos presentaron MAM grave (9.5%) y requirieron evacuación. La saturación de oxígeno en reposo a 2 700m no fue significativa para clasificar sujetos que luego desarrollaron MAM grave. Por el contrario, la asociación de desaturación durante el ejercicio a 2 700m más la saturación inapropiada en reposo a 4 300m fue significativa para clasificar a los sujetos que desarrollaron MAM grave con un valor predictivo positivo de 80% y un valor predictivo negativo del 97%. Nuestros resultados son relevantes para el montañismo y sugieren la adición de un simple test de ejercicio en la predicción del MAM grave. PMID:20228017

  2. EL PROTEOMA DEL TEJIDO ADIPOSO SUBCUTÁNEO MUESTRA HETEROGENEIDAD ANATÓMICA

    PubMed Central

    Martos-Moreno, G.A.; Sackmann-Sala, L.; Berryman, D.E.; Blome, D.W.; Argente, J.; Kopchick, y J.J.

    2015-01-01

    Introducción El tejido adiposo blanco (TAB) subcutáneo (Sc) humano podría variar dependiendo de su localización anatómica, con diferencias en su perfil proteómico. Pacientes y métodos Se obtuvieron aspirados de TAB-Sc de seis mujeres con IMC >25 kg/m2, sometidas a liposucción. Dicho TAB-Sc se obtuvo de seis localizaciones anatómicas: abdominal superior e inferior, muslo, dorsal, flanco y cadera, analizándose su perfil proteómico mediante electroforesis bidimensional. En muslo y abdomen superior se compararon, además, las muestras obtenidas de las dos capas del TAB-Sc (profunda y superficial). Resultados Se detectaron 21 proteínas que mostraban una intensidad de expresión diferente entre las seis localizaciones anatómicas y 14 entre las capas superficial y profunda de una misma región. Entre las proteínas identificadas se incluyen: vimentina (proteína estructural); proteínas “heat-shock” (HSPs), superóxido-dismutasa, (estrés/chaperoninas); proteína fijadora de ácidos grasos 4 (FABP-4) y alfa-enolasa (metabolismo lipídico y de los hidratos de carbono, respectivamente) y ATP-sintetasa (producción de energía). Entre las regiones estudiadas, el TAB-Sc dorsal mostraba un perfil proteómico particular, con menor expresión de proteínas implicadas en la producción de energía y metabolismo (ATP-sintetasa, alfa-enolasa, HSPs y FABP-4) que el resto de regiones. Conclusiones Los niveles de expresión de diversas proteínas en el TAB-Sc humano no son homogéneos, difiriendo entre localizaciones anatómicas. Esto sugiere la existencia de diferencias funcionales en el TAB-Sc de acuerdo con su localización anatómica, lo que debe considerarse antes de asumir la extrapolación de los datos derivados del TAB-Sc de una determinada localización al de otras partes de la anatomía. PMID:23228439

  3. PREVENCIÓN DEL VIH/SIDA EN LOS CIRCUITOS DE LEVANTE HSH: UNA ASIGNATURA PENDIENTE1

    PubMed Central

    Barreda, Victoria; Carballo-Dieguez, Alex; Marone, Rubén; Balán, Iván; Pando, María Ángeles; Ávila, María Mercedes

    2011-01-01

    Resumen A partir de un relevamiento de tipo etnográfico, se describen lugares de encuentro de HSH en la Ciudad de Buenos Aires y sus prácticas sexuales. El reconocimiento de tales espacios, así como las características que asumen en ellos los encuentros sexuales entre los HSH, plantean obstáculos específicos en la adopción de comportamientos preventivos y, asimismo, generan nuevos desafíos para las actividades de prevención. Se plantean las dificultades y debates conceptuales que la misma categoría presenta, y sus consecuencias en el abordaje preventivo y teórico-metodológico para las ciencias sociales. Además, se proponen nuevos interrogantes acerca de los alcances y las limitaciones del modelo preventivo del VIH/Sida para HSH. PMID:21874154

  4. Butyltin and PAH Contamination of Mar del Plata Port (Argentina) Sediments and Their Influence on Adjacent Coastal Regions.

    PubMed

    Laitano, María V; Castro, Ítalo B; Costa, Patrícia G; Fillmann, G; Cledón, M

    2015-10-01

    The distribution of butyltins (BTs) and polycyclic aromatic hydrocarbons (PAHs) were studied in surface sediments to assess how relevant is Mar del Plata port (Argentina) as a source of contamination to the surrounding environments. Within the port, TBT concentrations ranged from 24.2 to 150 ng Sn g(-1) and PAHs (Σ16) from 180 to 17,094 ng g(-1). At the surrounding beaches, PAHs were detected at low concentrations and TBT concentrations reached 10.9 ng Sn g(-1). Although those low levels indicate that the Port might not be an important source of contamination to the surrounding beaches, the very low TOC content and the coarse grain size of the beaches sediments could explain the sedimentary levels. The results show a reduction in TBT levels in Mar del Plata port after national and international use restrictions of TBT-based antifouling paints. PMID:26310126

  5. Argentinean adaptation of the Social Skills Inventory IHS-Del-Prette.

    PubMed

    Olaz, Fabián Orlando; Medrano, Leonardo; Greco, María Eugenia; Del Prette, Zilda Aparecida Pereira

    2009-11-01

    We present the results of the adaptation of the IHS-Del-Prette (Inventario de Habilidades Sociales, in English, Social Skills Inventory) to a sample of Argentinean college students. Firstly, we addressed the backward translation and carried out an equivalence study of the Portuguese and Spanish versions of the scale. The results showed the two versions were equivalent, as we obtained correlations lower than .50 in only 5 items. Secondly, we performed item analysis by calculating discrimination indexes and item-total correlations. Results indicated that the items are sensitive to differentiate between high and low social-skill groups. Exploratory factor analysis carried out with a sample of 602 college students yielded five factors that explained 26.5% of the total variance, although our data did not completely match the original factor structure. We also obtained moderate alpha values for the subscales, but high reliability for the total scale. Lastly, group differences between males and females are presented to provide evidence of validity. We discuss the implications of the results and present future lines of inquiry. PMID:19899676

  6. Late Tertiary northwestward-vergent thrusting in Valle del Cauca, Colombian Andes

    SciTech Connect

    Alfonso, C.A.; Sacks, P.E.; Secor, D.T. Jr.; Cordoba, F.

    1989-03-01

    The Valle del Cauca is a topographic basin situated between the Cordillera Central and the Cordillera Occidental in the Colombian Andes. The basement is Mesozoic mafic igneous rock of the Volcanic and Amaime Formations and clastic sediments and chert of the Espinal and Cisneros Formations. The basement was intruded by middle Cretaceous granodiorites (including the Batolito de Buga) and was deformed and metamorphosed to greenschist facies. The Mesozoic rocks originated in an oceanic setting and were accreted to northwestern South America during the Cretaceous or early Tertiary. Unconformably overlying the Mesozoic basement are the Eocene and Oligocene Vijes (marine limestone) and Guachinte and Cinta de Piedra (fluvial and deltaic sandstone and mudstone). In the Cordillera Central, the Cinta de Piedra is unconformably overlain by fanglomerate of the Miocene La Paila Formation. These clastics coarsen and thicken eastward. Geologic mapping and structural analyses show that the Mesozoic basement and its Tertiary cover are faulted and folded. Folds are asymmetric and overturned westward. Faults dip at shallow to moderate angles to the east and carry older sedimentary or basement rocks westward over younger rocks.

  7. Estudio ab initio del mecanismo de la reacción HSO + O3

    NASA Astrophysics Data System (ADS)

    Nebot Gil, I.

    La reacción entre el radical HSO y el ozono ha sido ampliamente estudiada desde el punto de vista experimental debido a la importancia que tiene el radical HSO en la oxidación de los compuestos de azufre reductores y a que puede contribuir a la producción de H2SO4 [1-4]. Se realizaron diversos estudios teóricos sobre la cinética de la reacción entre el radical HSO y el ozono. La reacción del HSO con el ozono presenta tres canales diferentes : HSO + O3 &rightarrow &HSO2 + O2 &rightarrow &HS + 2 O2 &rightarrow &SO + OH + O2 La controversia existente entre los grupos experimentales sobre cuál de las tres vías es la predominante, se ha resuelto mediante un estudio teórico de todas ellas utilizando métodos ab initio. La estructura de todos los reactivos, productos, intermedios y estados de transición ha sido optimizada a nivel ab initio utilizando los métodos UMP2 /6-31G** y QCISD/6-31G**.

  8. Afebrile pneumonia (whooping cough) syndrome in infants at Hospital Universitario del Valle, Cali, 2001-2007

    PubMed Central

    Villegas, Dolly; Echanda-Villegas, Connie Alejandra

    2012-01-01

    Introduction: Afebrile pneumonia syndrome in infants, also called infant pneumonitis, pneumonia caused by atypical pathogens or whooping cough syndrome is a major cause of severe lower respiratory infection in young infants, both in developing countries and in developed countries. Objective: To describe children with afebrile pneumonia syndrome. Methods: Through a cross-sectional study, we reviewed the medical records of children diagnosed with afebrile pneumonia treated at Hospital Universitario del Valle, a reference center in southwestern Colombia, between June 2001 and December 2007. We obtained data on maternal age and origin, prenatal care, the childs birth, breastfeeding, vaccination status, symptoms, signs, diagnosis, treatment, and complications. Results: We evaluated 101 children with this entity, noting a stationary presentation: June-August and November- December. A total of 73% of the children were under 4 months of age; the most common symptoms were: cyanotic and spasmodic cough (100%), respiratory distress (70%), and unquantified fever (68%). The most common findings: rales (crackles) (50%), wheezing and expiratory stridor (37%); 66% were classified as mild and of the remaining 33%, half of them required attention in the intensive care unit. In all, there was clinical diagnosis of afebrile pneumonia syndrome in infants, but no etiologic diagnosis was made and despite this, 94% of the children received macrolides. Conclusions: These data support the hypothesis that most of these patients acquired the disease by airway, possibly caused by viral infection and did not require the indiscriminate use of macrolides. PMID:24893051

  9. Explosive lithium production in the classical nova V339 Del (Nova Delphini 2013)

    NASA Astrophysics Data System (ADS)

    Tajitsu, Akito; Sadakane, Kozo; Naito, Hiroyuki; Arai, Akira; Aoki, Wako

    2015-02-01

    The origin of lithium (Li) and its production process have long been uncertain. Li could be produced by Big Bang nucleosynthesis, interactions of energetic cosmic rays with interstellar matter, evolved low-mass stars, novae, and supernova explosions. Chemical evolution models and observed stellar Li abundances suggest that at least half the Li may have been produced in red giants, asymptotic giant branch (AGB) stars, and novae. No direct evidence, however, for the supply of Li from evolved stellar objects to the Galactic medium has hitherto been found. Here we report the detection of highly blue-shifted resonance lines of the singly ionized radioactive isotope of beryllium, 7Be, in the near-ultraviolet spectra of the classical nova V339 Del (Nova Delphini 2013) 38 to 48 days after the explosion. 7Be decays to form 7Li within a short time (half-life of 53.22 days). The 7Be was created during the nova explosion via the alpha-capture reaction 3He(?,?)7Be (ref. 5). This result supports the theoretical prediction that a significant amount of 7Li is produced in classical nova explosions.

  10. Meteorological simulations of ozone episode case days during the 1996 Paso del Norte ozone study

    SciTech Connect

    Brown, M.J.; Costigan, K.; Muller, C.; Wang, G.

    1999-07-01

    Meteorological simulations centered around the border cities of El Paso and Ciudad Juarez have been performed during an ozone episode that occurred on Aug. 13, 1996 during the 1996 Paso del Norte Ozone Study field campaign. Simulations were performed using the HOTMAC mesoscale meteorological model using a 1, 2, 4, and 8 km horizontal grid size nested mesh system. Investigation of the vertical structure and evolution of the atmospheric boundary layer for the Aug. 11--13 time period is emphasized in this paper. Comparison of model-produced wind speed profiles to rawinsonde and radar profiler measurements shows reasonable agreement. A persistent upper-level jet was captured in the model simulations through data assimilation. In the evening hours, the model was not able to produce the strong wind direction shear seen in the radar wind profiles. Based on virtual potential temperature profile comparisons, the model appears to correctly simulate the daytime growth of the convective mixed layer. However, the model underestimates the cooling of the surface layer at night. The authors found that the upper-level jet significantly impacted the turbulence structure of the boundary layer, leading to relatively high turbulent kinetic energy (tke) values aloft at night. The model indicates that these high tke values aloft enhance the mid-morning growth of the boundary layer. No upper-level turbulence measurements were available to verify this finding, however. Radar profiler-derived mixing heights do indicate relatively rapid morning growth of the mixed layer.

  11. Block modeling of crustal deformation in Tierra del Fuego from GNSS velocities

    NASA Astrophysics Data System (ADS)

    Mendoza, L.; Richter, A.; Fritsche, M.; Hormaechea, J. L.; Perdomo, R.; Dietrich, R.

    2015-05-01

    The Tierra del Fuego (TDF) main island is divided by a major transform boundary between the South America and Scotia tectonic plates. Using a block model, we infer slip rates, locking depths and inclinations of active faults in TDF from inversion of site velocities derived from Global Navigation Satellite System observations. We use interseismic velocities from 48 sites, obtained from field measurements spanning 20 years. Euler vectors consistent with a simple seismic cycle are estimated for each block. In addition, we introduce far-field information into the modeling by applying constraints on Euler vectors of major tectonic plates. The difference between model and observed surface deformation near the Magallanes Fagnano Fault System (MFS) is reduced by considering finite dip in the forward model. For this tectonic boundary global plate circuits models predict relative movements between 7 and 9 mm yr- 1, while our regional model indicates that a strike-slip rate of 5.9 ± 0.2 mm yr- 1 is accommodated across the MFS. Our results indicate faults dipping 66- 4+ 6° southward, locked to a depth of 11- 5+ 5 km, which are consistent with geological models for the MFS. However, normal slip also dominates the fault perpendicular motion throughout the eastern MFS, with a maximum rate along the Fagnano Lake.

  12. Sensitivity Modeling Study for an Ozone Occurrence during the 1996 Paso Del Norte Ozone Campaign

    PubMed Central

    Lu, Duanjun; Reddy, Remata S.; Fitzgerald, Rosa; Stockwell, William R.; Williams, Quinton L.; Tchounwou, Paul B.

    2008-01-01

    Surface ozone pollution has been a persistent environmental problem in the US and Europe as well as the developing countries. A key prerequisite to find effective alternatives to meeting an ozone air quality standard is to understand the importance of local anthropogenic emissions, the significance of biogenic emissions, and the contribution of long-range transport. In this study, an air quality modeling system that includes chemistry and transport, CMAQ, an emission processing model, SMOKE, and a mesoscale numerical meteorological model, WRF, has been applied to investigate an ozone event occurring during the period of the 1996 Paso del Norte Ozone Campaign. The results show that the modeling system exhibits the capability to simulate this high ozone occurrence by providing a comparable temporal variation of surface ozone concentration at one station and to capture the spatial evolution of the event. Several sensitivity tests were also conducted to identify the contributions to high surface ozone concentration from eight VOC subspecies, biogenic VOCs, anthropogenic VOCs and long-range transportation of ozone and its precursors. It is found that the reductions of ETH, ISOP, PAR, OLE and FORM help to mitigate the surface ozone concentration, and like anthropogenic VOCs, biogenic VOC plays a nonnegligible role in ozone formation. But for this case, long-range transport of ozone and its precursors appears to produce an insignificant contribution. PMID:19190351

  13. Sensitivity modeling study for an ozone occurrence during the 1996 Paso Del Norte Ozone Campaign.

    PubMed

    Lu, Duanjun; Reddy, Remata S; Fitzgerald, Rosa; Stockwell, William R; Williams, Quinton L; Tchounwou, Paul B

    2008-12-01

    Surface ozone pollution has been a persistent environmental problem in the US and Europe as well as the developing countries. A key prerequisite to find effective alternatives to meeting an ozone air quality standard is to understand the importance of local anthropogenic emissions, the significance of biogenic emissions, and the contribution of long-range transport. In this study, an air quality modeling system that includes chemistry and transport, CMAQ, an emission processing model, SMOKE, and a mesoscale numerical meteorological model, WRF, has been applied to investigate an ozone event occurring during the period of the 1996 Paso del Norte Ozone Campaign. The results show that the modeling system exhibits the capability to simulate this high ozone occurrence by providing a comparable temporal variation of surface ozone concentration at one station and to capture the spatial evolution of the event. Several sensitivity tests were also conducted to identify the contributions to high surface ozone concentration from eight VOC subspecies, biogenic VOCs, anthropogenic VOCs and long-range transportation of ozone and its precursors. It is found that the reductions of ETH, ISOP, PAR, OLE and FORM help to mitigate the surface ozone concentration, and like anthropogenic VOCs, biogenic VOC plays a nonnegligible role in ozone formation. But for this case, long-range transport of ozone and its precursors appears to produce an insignificant contribution. PMID:19190351

  14. Structure of the Pliocene Camp dels Ninots maar-diatreme (Catalan Volcanic Zone, NE Spain)

    NASA Astrophysics Data System (ADS)

    Oms, O.; Bolós, X.; Barde-Cabusson, S.; Martí, J.; Casas, A.; Lovera, R.; Himi, M.; Gómez de Soler, B.; Campeny Vall-Llosera, G.; Pedrazzi, D.; Agustí, J.

    2015-11-01

    Maar volcanoes expose shallower or deeper levels of their internal structure as a function of the degree of erosion. In El Camp dels Ninots maar-diatreme (Catalan Volcanic Zone, Spain), the tephra ring has been largely eroded, and the remaining volcanic deposits infilling the diatreme are hidden under a lacustrine sedimentary infill of the crater. The volcano shows hardly any exposure, so its study needs the application of direct (e.g., boreholes) and indirect (shallow geophysics) subsurface exploration techniques. Additionally, this maar-diatreme was built astride two different substrates (i.e., mixed setting) as a result of its location in a normal fault separating Neogene sediments from Paleozoic granites. In order to characterize the internal structure and post-eruption stratigraphy of the maar-diatreme, we did geological studies (mapping, continuous core logging, and description of the tephra ring outcrops) and near-surface geophysics, including nine transects of electric resistivity tomography and a gravity survey. Results show that the deeper part of the diatreme is excavated into granites and is relatively steep and symmetrical. The uppermost diatreme is asymmetrical because of mechanical contrast between granites and Pliocene sands. The maar crater contained a lake permanently isolated from the surrounding relief and was deep enough to host anoxic bottom waters while its margins had shallower waters. These lake conditions preserved the remarkable Pliocene fossil record found in the lacustrine sediments.

  15. Using DelPhi capabilities to mimic conformational reorganization with amino acid specific dielectric constants

    NASA Astrophysics Data System (ADS)

    Wang, Lin; Sarhar, Subhra; Rocchia, Walter; Alexov, Emil

    2011-03-01

    Many molecular events are associated with small or large conformational changes occurring in the corresponding proteins. Modeling such changes is a challenge and requires significant amount of computing time. From point of view of electrostatics, these changes can be viewed as a reorganization of local charges and dipoles in response to the changes of the electrostatic field, if the cause is insertion or deletion of a charged amino acid. Here we report a large scale investigation of modeling the changes of the folding energy due to single mutations involving charge group. This allows the changes of the folding energy to be considered mostly electrostatic in origin and to be calculated with DelPhi assigning residue-specific value of the internal dielectric constant of protein in the range from 2 to 20. The predicted energy changes are benchmarked against experimentally measured changes of the folding energy on a set of 300 single mutations. The best fit between experimental values and predicted changes is used to find out the effective value of the internal dielectric constant for each type of amino acid. The work was supported by a grant from NIGMS, NIH, grant number 1R01GM093937-01.

  16. The Influence of Photolysis Rate Constants in Ozone Production for the Paso del Norte Region

    NASA Astrophysics Data System (ADS)

    Becerra, Fernando; Fitzgerald, Rosa

    2012-03-01

    In this research work we are focusing on understanding the relationship between photolysis rates and the photochemical ozone changes observed in the Paso del Norte region. The city of El Paso, Texas together with Ciudad Juarez, Mexico, forms the largest contiguous bi-national metropolitan area. This region suffers year-round ozone pollution events, and a better understanding is needed to mitigate them. Previous studies have found that ambient ozone concentrations tend to be higher on weekends rather than on weekdays, this phenomenon being referred to, as the ``weekend effect.'' If the ozone standard is exceeded more frequently on weekends, then this phenomenon must be considered in the design of ozone control strategies. In this work we investigate some of the most representative weekend ozone episodes at El Paso, TX, during the years 2009, 2010 and 2011 using the ozone photolysis rates. In this research the TUV radiative-transfer model is used to calculate the local photolysis rates and a UV MFRSR instrument is used to obtain experimental parameters. Seasonal variations and the weekday-weekend effect is studied. The results of this research will help to understand the underlying behavior of the photolysis rate constants when different atmospheric conditions are present.

  17. Meteorological Simulations of Ozone Episode Case Days during the 1996 Paso del Norte Ozone Study

    SciTech Connect

    Brown, M.J.; Costigan, K.; Muller, C.; Wang, G.

    1999-02-01

    Meteorological simulations centered around the border cities of El Paso and Ciudad Juarez have been performed during an ozone episode that occurred on Aug. 13,1996 during the 1996 Paso del Norte Ozone Study field campaign. Simulations were petiormed using the HOTMAC mesoscale meteorological model using a 1,2,4, and 8 km horizontal grid size nested mesh system. Investigation of the vertical structure and evolution of the atmospheric boundary layer for the Aug. 11-13 time period is emphasized in this paper. Comparison of model-produced wind speed profiles to rawirisonde and radar profiler measurements shows reasonable agreement. A persistent upper-level jet was captured in the model simulations through data assimilation. In the evening hours, the model was not able to produce the strong wind direction shear seen in the radar wind profiles. Based on virtual potential temperature profile comparisons, the model appears to correctly simulate the daytime growth of the convective mixed layer. However, the model underestimates the cooling of the surface layer at night. We found that the upper-level jet significantly impacted the turbulence structure of the boundary layer, leading to relatively high turbulent kinetic energy (tke) values aloft at night. The model indicates that these high tke values aloft enhance the mid-morning growth of the boundary layer. No upper-level turbulence measurements were available to verify this finding, however. Radar profiler-derived mixing heights do indicate relatively rapid morning growth of the mixed layer.

  18. Characterization of Triatoma guasayana biotopes in a hardwood forest of Santiago del Estero, Argentina.

    PubMed

    Vezzani, D; Schweigmann, N J; Pietrokovsky, S M; Wisnivesky-Colli, C

    2001-05-01

    Triatoma guasayana is a silvatic triatomine species distributed in Argentina, Bolivia and Paraguay. The study was performed in a secondary forest of Santiago del Estero, Argentina. The abundance of T. guasayana was evaluated by census in the following wild biotopes: quimiles (Opuntia quimilo), chaguares (dry bromeliads), logs and underground burrows. Ten biotopes of each type were dismantled in winter (August) and another 40 in summer (January); all fauna was recorded. The biotopes most infested by T. guasayana were quimiles (65%), followed by chaguares (55%), and logs (25%). Quimiles and chaguares were infested in both seasons, whereas logs were positive only in summer and burrows were never infested. Infestation and abundance were higher in summer than in winter. The biotope structure is a key factor for T. guasayana colonization. The larger number of refuges, the constant presence of blood sources and suitable inner microclimatic conditions offered by quimiles may favour the persistence of T. guasayana colonies. The richness of invertebrate fauna per type of biotope was ranked in the same order as that of T. guasayana, suggesting similar microhabitat requirements for all studied arthropods. PMID:11391416

  19. Selection of time-variant features for earthquake classification at the Nevado-del-Ruiz volcano

    NASA Astrophysics Data System (ADS)

    Cárdenas-Peña, David; Orozco-Alzate, Mauricio; Castellanos-Dominguez, German

    2013-02-01

    Seismic event recognition is an important task for hazard assessment, eruption prediction and risk mitigation, since it can be used to determine the state of a volcano. Usually, expert technicians read features extracted from the seismogram, such as, cepstral derived coefficients, energy centroids, instant frequency, instant envelop, among others. However, there are few studies about the selection of important features for classifying several types of seismic events, i.e., taking into account the temporal contribution of each considered feature. This paper presents a feature selection strategy based on a relevance measure of time-variant features for seismic event classification. In this research, features are selected as those with the maximal information preserved within the time analysis. Since features selection stage is performed by incremental training, a simple k-nearest neighbor classification rule is used to properly determine the dimension of the final feature set. The employed feature extraction and feature selection methodologies are tested on an isolated event recognition task. The database used to test the methodology is composed of the following classes: volcano-tectonic, long period earthquakes, tremors and hybrid events. Data was recorded at the seismic monitoring stations located at the Nevado-del-Ruiz volcano, Colombia. Using a classifier based on hidden Markov models, accomplished results exhibit a performance improvement from 78% to 88% using the proposed methodology in comparison to the state-of-the-art feature sets.

  20. RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes

    PubMed Central

    Wons, Ewa; Furmanek-Blaszk, Beata; Sektas, Marian

    2015-01-01

    DNA-dependent T7 RNA polymerase (T7 RNAP) is the most powerful tool for both gene expression and in vitro transcription. By using a Next Generation Sequencing (NGS) approach we have analyzed the polymorphism of a T7 RNAP-generated mRNA pool of the mboIIM2 gene. We find that the enzyme displays a relatively high level of template-dependent transcriptional infidelity. The nucleotide misincorporations and multiple insertions in A/T-rich tracts of homopolymers in mRNA (0.20 and 0.089%, respectively) cause epigenetic effects with significant impact on gene expression that is disproportionally high to their frequency of appearance. The sequence-dependent rescue of single and even double InDel frameshifting mutants and wild-type phenotype recovery is observed as a result. As a consequence, a heterogeneous pool of functional and non-functional proteins of almost the same molecular mass is produced where the proteins are indistinguishable from each other upon ordinary analysis. We suggest that transcriptional infidelity as a general feature of the most effective RNAPs may serve to repair and/or modify a protein function, thus increasing the repertoire of phenotypic variants, which in turn has a high evolutionary potential. PMID:25824942

  1. Albright hereditary osteodystrophy and del(2)(q37.3) in four unrelated individuals

    SciTech Connect

    Phelan, M.C.; Rogers, R.C.; Clarkson, K.B.

    1995-07-31

    Albright hereditary osteodystrophy (AHO) is a condition with characteristic physical findings (short stature, obesity, round face, brachydactyly) but variable biochemical changes (pseudohypoparathyroidism, pseudopseudohypoparathyroidism). Most patients with AHO have decreased activity of the guanine nucleotide-binding protein (G{sub s} protein) that stimulates adenylyl cyclase. The gene encoding the a subunit of the G{sub s} protein (GNAS1) has been mapped to the long arm of chromosome 20. We describe 4 unrelated individuals with apparent AHO, associated with small terminal deletions of chromosome 2. All 4 patients had normal serum calcium levels consistent with pseudopseudohypoparathyroidism. Del(2)(q37) is the first consistent karyotypic abnormality that has been documented in AHO. The finding of the same small terminal deletion in 4 unrelated individuals with a similar phenotype suggests that a gene locus in the 2q37 region is important in the pathogenesis of Albright syndrome. The association of Albright syndrome and the GNAS1 locus on chromosome 20 is well documented. The observation of a second potential disease locus on chromosome 2 may help explain the heterogeneity observed in this disorder. 44 refs., 7 figs.

  2. RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes.

    PubMed

    Wons, Ewa; Furmanek-Blaszk, Beata; Sektas, Marian

    2015-04-30

    DNA-dependent T7 RNA polymerase (T7 RNAP) is the most powerful tool for both gene expression and in vitro transcription. By using a Next Generation Sequencing (NGS) approach we have analyzed the polymorphism of a T7 RNAP-generated mRNA pool of the mboIIM2 gene. We find that the enzyme displays a relatively high level of template-dependent transcriptional infidelity. The nucleotide misincorporations and multiple insertions in A/T-rich tracts of homopolymers in mRNA (0.20 and 0.089%, respectively) cause epigenetic effects with significant impact on gene expression that is disproportionally high to their frequency of appearance. The sequence-dependent rescue of single and even double InDel frameshifting mutants and wild-type phenotype recovery is observed as a result. As a consequence, a heterogeneous pool of functional and non-functional proteins of almost the same molecular mass is produced where the proteins are indistinguishable from each other upon ordinary analysis. We suggest that transcriptional infidelity as a general feature of the most effective RNAPs may serve to repair and/or modify a protein function, thus increasing the repertoire of phenotypic variants, which in turn has a high evolutionary potential. PMID:25824942

  3. Study of the Local Horizon. (Spanish Title: Estudio del Horizonte Local.) Estudo do Horizonte Local

    NASA Astrophysics Data System (ADS)

    Ros, Rosa M.

    2009-12-01

    The study of the horizon is fundamental to easy the first observations of the students at any education center. A simple model, to be developed in each center, allows to easy the study and comprehension of the rudiments of astronomy. The constructed model is presented in turn as a simple equatorial clock, other models (horizontal and vertical) may be constructed starting from it. El estudio del horizonte es fundamental para poder facilitar las primeras observaciones de los alumnos en un centro educativo. Un simple modelo, que debe realizarse para cada centro, nos permite facilitar el estudio y la comprensin de los primeros rudimentos astronmicos. El modelo construido se presenta a su vez como un sencillo modelo de reloj ecuatorial y a partir de l se pueden construir otros modelos (horizontal y vertical). O estudo do horizonte fundamental para facilitar as primeiras observaes dos alunos num centro educativo. Um modelo simples, que deve ser feito para cada centro, permite facilitar o estudo e a compreenso dos primeiros rudimentos astronmicos. O modelo construdo apresenta-se, por sua vez, como um modelo simples de relgio equatorial e a partir dele pode-se construir outros modelos (horizontal e vertical)

  4. Structure and chemical composition of the envelope of Nova V339 del in the nebular phase

    NASA Astrophysics Data System (ADS)

    Tarasova, T. N.; Skopal, A.

    2016-01-01

    Based on our spectrophotometric observations, we have investigated the envelope of Nova V339 Del in the nebular phase. Our modeling of the H α line profiles and their comparison with the observed profiles have shown that the Nova envelope consists of circumpolar ejecta and a disk-shaped central component. The inclination of the orbital plane of the binary system, 65°, has been determined in the same way. We have estimated the mean electron density to be ~106 cm-3. Our estimates of the abundances of some chemical elements in the Nova envelope have shown that the concentrations of helium, neon, and iron are nearly solar, while the concentrations of nitrogen and oxygen exceed the solar ones by a factor of 120 ± 60 and 8 ± 1.6, respectively. The mass of the emission envelope in the nebular phase (from 253 to 382 days after the brightness maximum) has been estimated to be ~7 × 10-5 M ⊙.

  5. Isolation and assessment of phytate-hydrolysing bacteria from the DelMarVa Peninsula.

    PubMed

    Hill, Jane E; Kysela, David; Elimelech, Menachem

    2007-12-01

    The Delaware-Maryland-Virginia (DelMarVa) Peninsula, flanking one side of the Chesapeake Bay, is home to a substantial broiler chicken industry. As such, it produces a significant amount of manure that is typically composted and spread onto local croplands as a fertilizer. Phytate (myo inositol hexakisphosphate), the major form of organic phosphorus in the manure, can be hydrolysed by microorganisms to produce orthophosphate. Orthophosphate is a eutrophication agent which can lead to algal blooms, hypoxia and fish kills in the Chesapeake Bay and its tributaries. This transect study reveals a subpopulation of heterotrophic, thiosulfate-utilizing bacteria that can degrade phytate within the watershed as well as its receiving water sediment. Aerobic isolates were typical soil bacteria, e.g. Pseudomonad, Bacillus and Arthrobacter species, as well as a less common Staphylococcus inhabitant. Bacillus pumilus, Staphyloccocus equorum, Arthrobacter bergei and Pseudomonas marginalis strains have not been previously described as phytate-degrading. Each site along the transect - from manure pile to receiving sediment - was host to a population of bacteria that can degrade phytate and hence, each is a possible non-point source of orthophosphate pollution. Each new isolate could provide an enzyme additive for monogastric feed, thus reducing the impact of excessive phytate load on the environment. PMID:17991037

  6. Are pesticide residues associated to rice production affecting oyster production in Delta del Ebro, NE Spain?

    PubMed

    Ochoa, Victoria; Riva, Carmen; Faria, Melissa; Köck-Schulmeyer, Marianne; de Alda, Miren López; Barceló, Damià; Fernandez Tejedor, Margarita; Roque, Ana; Ginebreda, Antoni; Barata, Carlos

    2012-10-15

    Pesticide usage in Delta del Ebro (NE Spain) during the rice growing season has been associated with oyster episodes of mortality that occur early in summer. However, there are no studies that have directly evaluated pesticide levels and effects in oysters (Crassotrea gigas) cultured in Ebro's Bays. In this study pesticide levels in water, metal body burdens and up to 12 different biochemical markers were monitored in gills and digestive glands of oysters transplanted from May to June in 2008 and 2009. Biochemical responses evidenced clear differences in oysters from 2008 and 2009. Oysters transplanted in 2009 showed their antioxidant defenses unaffected from May to June and consequently increased levels of tissue damage measured as lipid peroxidation and DNA strand breaks and of mortality rates. Conversely oysters transplanted in 2008 increase their antioxidant defenses from May to June, had low levels of lipid peroxidation and DNA damage and low mortality rates. Some pesticides in water such as bentazone and propanil together with high temperatures and salinity levels were related with tissue damage in oyster transplanted in 2008 but the observed large differences between years indicate that abiotic factors alone could not explain the high mortalities observed in 2009. An analysis of recent reported studies pointed out in the direction that in addition to abiotic factors the use of oysters sensitive to diseases may explain the observed responses. PMID:22940045

  7. Fracture Characteristics in a Disposal Pit on Mesita del Buey, Los Alamos National Laboratory

    SciTech Connect

    David T. Vaniman; Steven L. Reneau

    1998-12-01

    The characteristics of fractures in unit 2 of the Tshirege Member of the Bandelier Tuff were documented in Pit 39, a newly excavated 13.7 m deep disposal pit at Material Disposal Area G on Mesita del Buey. The average spacing between fractures is about 1.0 to 1.3 m, the average fracture aperture is about 3 to 5 mm, and the average fracture dip is about 76o to 77o. Fracture spacing and dip in Pit 39 are generally consistent with that reported from other fracture studies on the Pajarito Plateau, although the fracture apertures in Pit 39 are less than reported elsewhere. Measured fracture orientations are strongly affected by biases imparted by the orientations of the pit walls, which, combined with a small data set, make identification of potential preferred orientations dlfflcult. The most prominent fracture orientations observed in Pit 39, about E-W and N20E, are often not well represented elsewhere on the Pajarito Plateau. Fracture fills contain smectite to about 3 m depth, and calcite and opal may occur at all depths, principally associated with roots or root fossils (rhizoliths). Roots of pifion pine extend in fractures to the bottom of the pit along the north side, perhaps indicating a zone of preferred infiltration of water. Finely powdered tuff with clay-sized particles occurs within a number of fractures and may record abrasive disaggregation associated with small amounts of displacement on minor local faults.

  8. Paleoseismic observations of an onshore transform boundary: The Magallanes-Fagnano fault, Tierra del Fuego, Argentina

    USGS Publications Warehouse

    Costa, C.H.; Smalley, R., Jr.; Schwartz, D.P.; Stenner, H.D.; Ellis, M.; Ahumada, E.A.; Velasco, M.S.

    2006-01-01

    We present preliminary information on the geomorphologic features and paleoseismic record associated with the ruptures of two Ms 7.8 earthquakes that struck Tierra del Fuego and the southernmost continental margin of South America on December 17, 1949. The fault scarp was surveyed in several places cast of Lago Fagnano and a trench across a secondary fault trace of the Magallanes-Fagnano fault was excavated at the Ri??o San Pablo. The observed deformation in a 9 kyr-old peat bog sequence suggests evidence for two, and possibly three pre-1949 paleoearthquakes is preserved in the stratigraphy. The scarp reaches heights up to 11 m in late Pleistocene-Holocence(?) deposits, but the vertical component of the 1949 events was always less than ???1 m. This observation also argues for the occurrence of previous events during the Quaternary. Along die part of the fault we investigated east of Lago Fagnano, the horizontal component of the 1949 rupture does not exceed 4 m and is likely lower than 0.4 m, which is consistent with the kinematics of a local releasing bend, or at the end of a strike-slip rupture zone. ?? 2006 Revista de la Asociacio??n Geolo??gica Argentina.

  9. Spectral characteristics of volcano-tectonic earthquake swarms in Nevado del Ruiz Volcano, Colombia

    NASA Astrophysics Data System (ADS)

    Londono, John M.; Sudo, Yasuaki

    2001-12-01

    Spectral analyses for volcano-tectonic earthquakes were carried out at Nevado del Ruiz Volcano (NRV) for the period 1985-1996 for several earthquake swarms around the volcano, named North, East, West, South and Crater swarm zones. Important spectral peaks for each earthquake swarm zone were found by counting the number of spectra that had the same spectral peaks. Each swarm zone showed some characteristic peaks, which could help to differentiate between them; however, the most important peaks were similar for all the zones. These results suggest that the earthquake swarms at NRV were influenced directly by the source (activity of the volcano) and could also be influenced by the site effect. Some temporal changes were observed in spectral parameters such as a change in the frequency contents in almost all the swarm zones, and the frequency of the P-waves in the West earthquake swarm zone. Before the eruptions on November 13, 1985 and September 1, 1989, P-waves showed low frequencies (1-2 Hz) at the West earthquake swarm. After the eruptions, the frequencies of P increased (2-4 Hz). This fact showed that changes (decreasing of frequencies) in the spectra of P-waves at the West earthquake swarm could help in the monitoring of volcanic activity at NRV. This swarm zone seems to be related directly with the most important volcanic crises that have occurred. This suggests that the West swarm zone should be monitored in more detail in the future.

  10. Explosive lithium production in the classical nova V339 Del (Nova Delphini 2013).

    PubMed

    Tajitsu, Akito; Sadakane, Kozo; Naito, Hiroyuki; Arai, Akira; Aoki, Wako

    2015-02-19

    The origin of lithium (Li) and its production process have long been uncertain. Li could be produced by Big Bang nucleosynthesis, interactions of energetic cosmic rays with interstellar matter, evolved low-mass stars, novae, and supernova explosions. Chemical evolution models and observed stellar Li abundances suggest that at least half the Li may have been produced in red giants, asymptotic giant branch (AGB) stars, and novae. No direct evidence, however, for the supply of Li from evolved stellar objects to the Galactic medium has hitherto been found. Here we report the detection of highly blue-shifted resonance lines of the singly ionized radioactive isotope of beryllium, (7)Be, in the near-ultraviolet spectra of the classical nova V339 Del (Nova Delphini 2013) 38 to 48 days after the explosion. (7)Be decays to form (7)Li within a short time (half-life of 53.22 days). The (7)Be was created during the nova explosion via the alpha-capture reaction (3)He(α,γ)(7)Be (ref. 5). This result supports the theoretical prediction that a significant amount of (7)Li is produced in classical nova explosions. PMID:25693569

  11. Baseline trace metals in gastropod mollusks from the Beagle Channel, Tierra del Fuego (Patagonia, Argentina).

    PubMed

    Conti, Marcelo Enrique; Stripeikis, Jorge; Finoia, Maria Grazia; Tudino, Mabel Beatriz

    2012-05-01

    With the aim to evaluate the mollusk Nacella (P)magellanica as biomonitor of elemental pollution in seawater of the Beagle Channel, more than one hundred individuals of the gastropod were sampled, separated in viscera and muscle, and then examined with respect to the accumulation of Cd, Cr, Cu, Ni, Pb and Zn. Collection was performed in seven strategic locations along 170 km of the coastal area of the Beagle Channel (Tierra del Fuego, Argentina) in two campaigns during 2005 and 2007. Samples of surrounding seawater in the different sites were obtained and tested for the same metals as well. The accumulation capacity of Nacella (P)magellanica and thus its aptitude as biomonitor, was evaluated through the calculus of the preconcentration factors of the metals assayed. A discussion involving the comparison with other mollusks previously tested will be given. Several statistical approaches able to analyze data with environmental purposes were applied. Non parametric univariate tests such as Kruskal-Wallis and Mann-Whitney were carried out to assess the changes of the metal concentrations with time (2005 and 2007) in each location. Multivariate methods (linear discriminant analysis on PCA factors) were also applied to obtain a more reliable site classification. Johnson's probabilistic method was carried out for comparison between different geographical areas. The possibility of employing these results as heavy metals' background levels of seawater from the Beagle Channel will be debated. PMID:22350107

  12. DISYUNTIVAS EN LAS CONCEPCIONES SOBRE AUTONOMÍA Y BENEFICENCIA QUE AFECTAN LA TERAPÉUTICA DEL INTENTO SUICIDA1

    PubMed Central

    Mondragón, Liliana; Monroy, Zuraya; Ito, Ma. Emily; Medina-Mora, Dra. Ma. Elena

    2010-01-01

    El objetivo del trabajo es conocer las disyuntivas entre los principios de beneficencia y autonomía, que se presentan en la relación médico-paciente, durante la terapéutica del intento de suicidio. La investigación se realizó en dos hospitales psiquiátricos de la Ciudad de México. La muestra incluyó a tres sujetos con intento de suicidio, mayores de 18 años, que eran atendidos en consulta externa a causa de una lesión autoinfligida en el último año, y a tres psiquiatras que trataban a estos pacientes. La información se obtuvo previo consentimiento informado en entrevistas individuales. Se llevó a cabo un análisis de discurso argumentado para encontrar los significados que los participantes otorgaron a los principios bioéticos y las posibles disyuntivas entre éstos. Las discordancias entre la beneficencia y la autonomía estuvieron relacionadas con el beneficio del tratamiento, el respeto por los valores y las creencias de los pacientes, entre otros. Este trabajo presenta consideraciones éticas relevantes en el escenario clínico, al ofrecer al psiquiatra un análisis bioético que le permita actuar de acuerdo con la beneficencia y respetando la autonomía del paciente frente a casos de intento de suicidio y, de esta forma procurar una mejor atención para ellos. PMID:20830214

  13. Inventario mundial de la calidad del carbon mineral (WoCQI) [The world coal quality inventory (WoCQI)

    USGS Publications Warehouse

    Finkelman, R.B.; Lovern, V.S.

    2001-01-01

    Los oficiales encargados de la politica comercial de cada pais requieren informacion clara y precisa sobre el recurso del carbon mineral, particularmente sobre sus propiedades y caracteristicas, para tomar decisiones bien fundamentadas con respecto al mejor uso de los recursos naturales, necesidades de importacion y oportunidades de exportacion, objetivos de politica interna y externa, oportunidades de transferencia tecnologica, posibilidades de inversion externa, estudios ambientales y de salud, y asuntos relacionados con el uso de productos secundarios y su disposicion.

  14. [Spirocamallanus hilarii (Nematoda, Camallanidae) fresh water fishes parasite from the dam of Termas de Ro Hondo, Santiago del Estero, Argentina].

    PubMed

    Ramallo, G

    1997-01-01

    Spirocamallanus hilarii (Nematoda, Camallanidae) is cited parasitizing five fresh water fish species: Salminus maxillosus Leporinus obtusidens, Hoplias malabaricus malabaricus, Oligosarcus jenynsii and Pimelodus albicans, captured in Termas de Ro Hondo pond, province of Santiago del Estero, Argentina. Morphologic and morphometric parasitological studies were done. The specimens described were drawned and photographed. In addition, the following parasitic infection indexes were calculated: Prevalence 26.5%, Mean 6.6 and Maximum Intensity 27. PMID:9640682

  15. Short- and long-term benefits of lenalidomide treatment in patients with lower-risk del(5q) myelodysplastic syndromes

    PubMed Central

    Komrokji, R. S.; List, A. F.

    2016-01-01

    The treatment of patients with myelodysplastic syndromes (MDS) begins with assessment of karyotype and risk. Lenalidomide is approved for the treatment of patients who have transfusion-dependent anemia due to lower-risk MDS with chromosome 5q deletion (del(5q)) with or without additional cytogenetic abnormalities, and isolated del(5q) only in the European Union. Mounting evidence suggests that lenalidomide is effective not only in reducing red blood cell (RBC) transfusion burden, but also in modifying the disease natural history by suppressing the malignant clone. Data discussed here from the pivotal phase 2 (MDS-003) and phase 3 (MDS-004) studies of lenalidomide demonstrate that lenalidomide treatment was associated with both short- and long-term benefits. These clinical benefits included high rates of RBC-transfusion independence (TI) with prolonged durations of response, high rates of cytogenetic response (CyR) associated with achievement of durable RBC-TI, no significant difference in rates of progression to acute myeloid leukemia (AML), and improvements in health-related quality of life (HRQOL). Achievement of RBC-TI and CyR with lenalidomide treatment was associated with extended survival and time to AML progression. Achievement of RBC-TI and hemoglobin response was additionally associated with HRQOL benefits. Recent data describing the impact of TP53 mutations and p53 expression on the prognosis of patients with del(5q) and the impact on response to lenalidomide are also discussed. The authors provide practical recommendations for the use of lenalidomide in patients with lower-risk del(5q) MDS. PMID:26504152

  16. El Informe a la Nación sobre el Estado del Cáncer, 1975-2012

    Cancer.gov

    El Informe a la Nación sobre el Estado del Cáncer (1975-2012) indica que continuó la baja de tasas de mortalidad de todos los cánceres juntos, así como de la mayoría de los cánceres en hombres y mujeres de todos los grupos raciales y étnicos principales.

  17. Terminal deletion of the long arm of chromosome 3 [46,XX,del(3)(q27{r_arrow}qter)

    SciTech Connect

    Chitayat, D.; Babul, R.; Silver, M.M.

    1996-01-02

    We report on a terminal deletion of the long arm of chromosome 3[46,XX,del(3)(q27{r_arrow}qter)] in a female newborn infant who died 45 hours after delivery and had multiple congenital abnormalities including bilateral anophthalmia, congenital heart disease, and abnormal genitalia. The findings are compared to those of four previously reported cases with terminal de (3q). 8 refs., 4 figs., 1 tab.

  18. Short- and long-term benefits of lenalidomide treatment in patients with lower-risk del(5q) myelodysplastic syndromes.

    PubMed

    Komrokji, R S; List, A F

    2016-01-01

    The treatment of patients with myelodysplastic syndromes (MDS) begins with assessment of karyotype and risk. Lenalidomide is approved for the treatment of patients who have transfusion-dependent anemia due to lower-risk MDS with chromosome 5q deletion (del(5q)) with or without additional cytogenetic abnormalities, and isolated del(5q) only in the European Union. Mounting evidence suggests that lenalidomide is effective not only in reducing red blood cell (RBC) transfusion burden, but also in modifying the disease natural history by suppressing the malignant clone. Data discussed here from the pivotal phase 2 (MDS-003) and phase 3 (MDS-004) studies of lenalidomide demonstrate that lenalidomide treatment was associated with both short- and long-term benefits. These clinical benefits included high rates of RBC-transfusion independence (TI) with prolonged durations of response, high rates of cytogenetic response (CyR) associated with achievement of durable RBC-TI, no significant difference in rates of progression to acute myeloid leukemia (AML), and improvements in health-related quality of life (HRQOL). Achievement of RBC-TI and CyR with lenalidomide treatment was associated with extended survival and time to AML progression. Achievement of RBC-TI and hemoglobin response was additionally associated with HRQOL benefits. Recent data describing the impact of TP53 mutations and p53 expression on the prognosis of patients with del(5q) and the impact on response to lenalidomide are also discussed. The authors provide practical recommendations for the use of lenalidomide in patients with lower-risk del(5q) MDS. PMID:26504152

  19. Ephemeral skin-flows on talus affected by permafrost degradation (Corral del Veleta, Spain)

    NASA Astrophysics Data System (ADS)

    Tanarro, L. M.; Palacios, D.; Zamorano, J. J.; Gómez, A.

    2009-04-01

    In mountain environments, talus formed at the foot of valley sides are frequently affected by hillslope processes, such as skin-flows. The main characteristic of this type of flow is that it only causes the movement of a thin layer of soil, regolith or debris over an inclined plane parallel to the topographical surface of the talus. Some examples of this movement have already been described (Rapp, 1960; Akerman; 1984; White, 1981, Benedict, 1970; Jahn, 1967; Söderman, 1980; Lewkowicz, 1988, Harris, 1987). The causes of the formation of these movements vary, but in general they have been linked primarily with rapid snow melt and/or with degraded permafrost levels (Jahn, 1967; Nyberg, 1991; Rapp and Nyberg, 1988; Strömquist, 1985; Hall, 1985; Thorn, 1988; McRoberts and Morgenstern, 1974, Caine, 1976). Within this context, the aims of this paper are: first, to present and describe the characteristics and temporal and geomorphological evolution of a series of skin-flows which have occurred on the talus which lies at the foot of the Corral del Veleta glacial cirque, and second, to analyze the factors that appear to have triggered them. The Pico del Veleta mountain (3398 m a.s.l.) is one of the main summits in the Sierra Nevada Massif, a group of mountains forming part of the Cordilleras Béticas, in SE Spain (37°03´N, 3°22´W). The Corral del Veleta is a glacial cirque on the northeastern face of the Pico del Veleta, c. 600 m long in a NW-SE direction, with a head is formed by a steep wall which falls more than 250 meters to meet with a wide and irregular talus. The cirque headwall is composed of metamorphic rocks (mainly micaschist), lined up in structural steps or shelves tilted towards the NNW. One of these shelves forms the base of the cirque, on which a series of moraine ridges, tardiglacial or from the Little Ice Age, which close off the cirque towards the North (Gómez et al., 2001). The talus is formed from abundant debris resulting from weathering (gelifraction) and hillslope dynamics (rockfall activity) which affect the headwall (Gómez et al., 2003) and is formed mainly by various talus cones which are irregular in shape and stepped as the accumulated debris covers the remains of the stepped structural shelves which were not destroyed by the glacial erosion. Although the talus debris is basically made up of blocks, it is important to point out the abundant presence of fine material, produced by the weathering of the micaschist. (Castillo and Fedeli, 2002; Gómez et al., 2003). Field work carried out over the last ten years (1998-2008) has allowed observation of the triggering and formation in some years of various skin flows in different sectors of the talus, especially in the late summer of 2002 when four skin flows occurred. Within this timeframe monitoring and analysis of this kind of skin flow has been carried out. On the one hand, the description of the morphology, morphometry and sedimentology of each flow has been completed with the production of detailed geomorphological mapping and from sedimentological analysis. The geomorphological mapping has, in turn, allowed the observation of the geomorphological evolution of the flows from the time they occurred. On the other hand, a study has been made of the variables or factors which seem a priori to control the triggering of the skin flow landslips: the snow melt and the presence of permafrost in the detrital talus. The former has been monitored through photographic control of the snow cover at the end of the summer season, so that for each date analyzed a map was obtained of the snow cover, superimposing in turn the location of the skin flows at that date. A GIS processing of the different snow covers has also allowed a map to be produced with the areas of maximum summer snow cover, which was compared with the sites of the skin flow landslips. The existence of permafrost and its presence in the detrital slope has been detected through the monitoring of the ground temperature (BTS measurements, miniature temperature dataloggers) and geophysical surveys (Gómez et al., 2001, 2003). However, these methods for detecting permafrost (pending more detailed surveys) have shown that the presence of permafrost or buried ice in the slope is discontinuous. The geomorphological interpretation shows that the flows are small scale. The maximum length of the largest flow occurring during the observation period is around 50 m, and its width oscillates between 25-30 m. In general the flow only affects a layer of debris of less than 30 cm. The dislodged layer is made up of fine material and small clasts or gravel and pebble sized fragments, with a significant presence of numerous multiple flows which diverge or divide when they encounter a large block and end up forming small lobes. The interior of these is made up of fine material (clay and silt) and the lobe itself is made of small, tightly compressed fragments. Here it should also be noted that many of the flows are stopped or ended when they collide with a block of larger size, forming a series of lobes. It seems clear that many of these flows, as they are moving only a thin layer of fine material, are controlled by the presence of larger blocks which remain stable on the slope. The location of the skin flows at the base of the snowpatches in late summer seems to be the main factor which triggers their genesis. Here, as has been observed in other areas, the absence of vegetation and the abundant water delivered by the rapid nival fusion or snow melt, stronger here as this is an area with Mediterranean climate and also linked to the presence of abundant fine material, provide the conditions needed to favor the triggering of this kind of movement. However it was observed that some skin flows develop in sectors of the slope where the permafrost is absent and so their origin seems to be clearly related to the snow melt. In addition in these cases, the morphology of the flows once they are formed is not destroyed but rather tends to ‘blur' as the years pass, although it does not completely lose its original shape: the fine material inside the lobes becomes compacted and the multiple lobes become blurred. In contrast, in other sectors of the slope, where the presence of permafrost levels or buried ice has been detected, the skin flows, once formed, change their physiognomy from year to year, which may be related to the influence of the nival dynamic along with the degradation of the permafrost and the dislodgement of the active layer. References Akerman, H, .J, 1984: Notes on talus morphology and processes in Spitsbergen. Geografiska Annaler 66A (4): 267-284. Benedict, J. B. 1970. Downslope soil movement in a Colorado alpine region: rates, processes, and climatic significance, Arctic and Alpine Research, 2(3): 165-226 Caine N (1976). The influence of snow and increased snowfall on contemporary geomorphic processes in alpine areas. In: Steinoff HW, Ives JD (eds) Ecological impacts of snowpack augmentation in the San Juan Mountains. Colorado State University, Fort Collins, pp 145-200 Castillo, A. and Fedeli, B. 2002. Algunas pautas del comportamiento hidrogeológico de rocas duras afectadas por glaciarismo y periglaciarismo en Sierra Nevada (España). Geogaceta, 32: 195-197. Gómez, A., Palacios, D., Ramos, M., Tanarro, L.M., Schulte, L and Salvador, F., 2001: Location of permafrost in marginal regions: Corral del Veleta, Sierra Nevada. Permafrost and Periglacial Processes, 12: 93-110. Gómez, A.; Palacios, D.; Luengo, E.; Tanarro, L. M.; Schulte, L. & Ramos, M. 2003. Talus instability in a recent deglaciation area and its relantionship to buried ice and snow cover evolution (Picacho del Veleta, Sierra Nevada, Spain). Geografiska Annaler, 85 A(2): 165-182. Hall, K., 1985: Some observations on ground temperatures and transport processes at a nivation site in northern Norway. Norsk Geografisk Tidsskrift, 39: 27-37. Harris, C., 1987: Mechanisms of mass movement in periglacial environments. In: Anderson, M.G. and Richards, K.S. (eds): Slope Stability. John Wiley and Sons. Chichester. 531-559. Jahn, A., 1967: Some features of mass movement on Spitsbergen slopes. Geografiska Annaler, 49A: 213-225. Lambiel, C. and Pieracci, K. 2008. Permafrost distribution in talus slopes located within the alpine periglacial belt, Swiss Alps. Permafrost and Periglacial Processes, 19: 293-304. Lewkowicz, A.G.1988: Slope processes. In: Clark, M.J. (eds): Advances in Periglacial Geomorphology. John Wiley and Sons. Chichester. 325-368. McRoberts, E.C. and Morgenstern, N.R., 1974: The stability of the thawing slopes. Canadian Geotechnical Journal, 11: 447-469. Nyberg, R., 1991: Geomorphic processes at snowpatch sites in the Abisko mountains, northern Sweden. Zeitschrift für Geomorphologie N.F., 35(3): 321-343. Rapp, A. and Nyberg, R., 1988: Mass movements, nivation processes and climatic fluctuations in northern Scandinavian mountains. Norsk Geografisk Tidsskrift, 42: 245-253. Rapp, A., 1960: Recent development of mountain slopes in Kärkevagge and surroundings, Northen Scandinavia. Geografiska Annaler, 42A(2-3): 1-199. Soderman, G., 1980: Slope processes in cold environments of northern Finland. Fennia, 158: 83-152. Strömquist, L., 1985: Geomorphic impact of snowmelt on slope erosion and sediment production. Zeitschrift für Geomorphologie N. F., 29: 129-138. Thorn, C. E., 1988: Nivation: a geomorphic chimera. In: Clark, M. J. (ed.): Advances in Periglacial Geomorphology: John Wiley and Sons. Chichester. 3-31. White, S.E. 1981: Alpine mass movement forms (non-catastrophic). Arctic and Alpine Research 13: 127-37.

  20. Is BRCA1-5083del19, identified in breast cancer patients of Sicilian origin, a Calabrian founder mutation?

    PubMed

    Russo, Antonio; Cal, Valentina; Bruno, Loredana; Schir, Valentina; Agnese, Valentina; Cascio, Sandra; Foddai, Elena; Fanale, Daniele; Rizzo, Sergio; Di Gaudio, Francesca; Gulotta, Eliana; Surmacz, Eva; Di Fede, Gaetana; Bazan, Viviana

    2009-01-01

    Various studies have been published in Italy regarding the different BRCA1 mutations, but only the BRCA1-5083del19 mutation is recurrent and specific to individuals of Italian descent with a founder effect on the Calabrian population. In our previous study, BRCA1-5083del19 mutation carriers were found in four index cases of 106 Sicilian patients selected for familial and/or hereditary breast/ovarian cancers. The high frequency rate of this mutation identified in the Sicilian population led us to perform haplotype analysis in all family carriers. Five highly polymorphic microsatellite markers were used (D17S1320, D17S932, D17S1323, D17S1326, D17S1325) to establish whether or not all these families had a common ancestor. This analysis showed that all mutation carriers of these families had a common allele. None of the non-carriers of the mutation or of the 50 healthy Sicilian controls showed this haplotype. This allelotype analysis highlighted the presence of a common allele (ancestor), thus suggesting the presence of a founder effect in the Sicilian population. Our results are in contrast with other studies but only the allelotype analysis of all the BRCA1-5083del19 mutation carriers of two neighboring regions of the south of Italy (Calabria and Sicily) will make it possible to identify the real ancestor of this mutation. PMID:18228134