Sample records for denshi jh tsshin

  1. JH & JH Properties, LLC Information Sheet

    EPA Pesticide Factsheets

    JH & JH Properties, LLC (the Company) is located in Fargo, North Dakota. The settlement involves renovation activities conducted at a property constructed prior to 1978, located in Fargo, North Dakota.

  2. Coordinated changes in JH biosynthesis and JH hemolymph titers in Aedes aegypti mosquitoes

    PubMed Central

    Hernández-Martínez, Salvador; Rivera-Perez, Crisalejandra; Nouzova, Marcela; Noriega, Fernando G.

    2014-01-01

    Juvenile hormone III (JH) is synthesized by the corpora allata (CA) and plays a key role in mosquito development and reproduction. A decrease in JH titer during the last instar larvae allows pupation and metamorphosis to proceed. As the anti-metamorphic role of JH comes to an end, the CA of the late pupa once again synthesizes JH, which plays an essential role in orchestrating reproductive maturation. In spite of the importance of Aedes aegypti as a vector, a detailed study of the changes of JH hemolymph titers during the gonotrophic cycle has never been performed. In the present studies, using a High Performance Liquid Chromatography coupled to a Fluorescent Detector (HPLC-FD) method, we measured changes in JH levels in the hemolymph of female mosquitoes during the pupal and adult stages. Our results revealed tightly concomitant changes in JH biosynthesis and JH hemolymph titers during the gonotrophic cycle of female mosquito. Feeding high sugar diets resulted in an increase of JH titers, and mating also modified JH titers in hemolymph. In addition these studies confirmed that JH titer in mosquitoes is fundamentally determined by the rate of biosynthesis in the CA. PMID:25445664

  3. Structural mechanism of JH delivery in hemolymph by JHBP of silkworm, Bombyx mori

    PubMed Central

    Suzuki, Rintaro; Fujimoto, Zui; Shiotsuki, Takahiro; Tsuchiya, Wataru; Momma, Mitsuru; Tase, Akira; Miyazawa, Mitsuhiro; Yamazaki, Toshimasa

    2011-01-01

    Juvenile hormone (JH) plays crucial roles in many aspects of the insect life. All the JH actions are initiated by transport of JH in the hemolymph as a complex with JH-binding protein (JHBP) to target tissues. Here, we report structural mechanism of JH delivery by JHBP based upon the crystal and solution structures of apo and JH-bound JHBP. In solution, apo-JHBP exists in equilibrium of multiple conformations with different orientations of the gate helix for the hormone-binding pocket ranging from closed to open forms. JH-binding to the gate-open form results in the fully closed JHBP-JH complex structure where the bound JH is completely buried inside the protein. JH-bound JHBP opens the gate helix to release the bound hormone likely by sensing the less polar environment at the membrane surface of target cells. This is the first report that provides structural insight into JH signaling. PMID:22355650

  4. Effects of juvenile hormone (JH) analog insecticides on larval development and JH esterase activity in two spodopterans.

    PubMed

    El-Sheikh, El-Sayed A; Kamita, Shizuo G; Hammock, Bruce D

    2016-03-01

    Juvenile hormone analog (JHA) insecticides are biological and structural mimics of JH, a key insect developmental hormone. Toxic and anti-developmental effects of the JHA insecticides methoprene, fenoxycarb, and pyriproxyfen were investigated on the larval and pupal stages of Spodoptera littoralis and Spodoptera frugiperda. Bioassays showed that fenoxycarb has the highest toxicity and fastest speed of kill in 2nd instar S. littoralis. All three JHAs affected the development of 6th instar (i.e., final instar) and pupal S. frugiperda. JH esterase (JHE) is a critical enzyme that helps to regulate JH levels during insect development. JHE activity in the last instar S. littoralis and S. frugiperda was 11 and 23 nmol min(-1) ml(-1) hemolymph, respectively. Methoprene and pyriproxyfen showed poor inhibition of JHE activity from these insects, whereas fenoxycarb showed stronger inhibition. The inhibitory activity of fenoxycarb, however, was more than 1000-fold lower than that of OTFP, a highly potent inhibitor of JHEs. Surprisingly, topical application of methoprene, fenoxycarb or pyriproxyfen on 6th instars of S. littoralis and S. frugiperda prevented the dramatic reduction in JHE activity that was found in control insects. Our findings suggest that JHAs may function as JH agonists that play a disruptive role or a hormonal replacement role in S. littoralis and S. frugiperda. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. The JH2 domain and SH2-JH2 linker regulate JAK2 activity: A detailed kinetic analysis of wild type and V617F mutant kinase domains.

    PubMed

    Sanz Sanz, Arturo; Niranjan, Yashavanthi; Hammarén, Henrik; Ungureanu, Daniela; Ruijtenbeek, Rob; Touw, Ivo P; Silvennoinen, Olli; Hilhorst, Riet

    2014-10-01

    JAK2 tyrosine kinase regulates many cellular functions. Its activity is controlled by the pseudokinase (JH2) domain by still poorly understood mechanisms. The V617F mutation in the pseudokinase domain activates JAK2 and causes myeloproliferative neoplasms. We conducted a detailed kinetic analysis of recombinant JAK2 tyrosine kinase domain (JH1) and wild-type and V617F tandem kinase (JH1JH2) domains using peptide microarrays to define the functions of the kinase domains. The results show that i) JAK2 follows a random Bi-Bi reaction mechanism ii) JH2 domain restrains the activity of the JH1 domain by reducing the affinity for ATP and ATP competitive inhibitors iii) V617F decreases affinity for ATP but increases catalytic activity compared to wild-type and iv) the SH2-JH2 linker region participates in controlling activity by reducing the affinity for ATP. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. NMR assignments of juvenile hormone binding protein in complex with JH III.

    PubMed

    Suzuki, Rintaro; Tase, Akira; Fujimoto, Zui; Shiotsuki, Takahiro; Yamazaki, Toshimasa

    2009-06-01

    A hemolymph juvenile hormone binding protein (JHBP) shuttles hydrophobic JH, a key hormone in regulation of the insect life cycle, from the site of the JH biosynthesis to the cells of target organs. We report complete NMR chemical shift assignments of Bombyx mori JHBP in the JH III-bound state.

  7. JH III production, titers and degradation in relation to reproduction in male and female Anthonomus grandis.

    PubMed

    Taub-Montemayor, Tina E; Min, Kyung-Jin; Chen, Zhaorigetu; Bartlett, Terri; Rankin, Mary Ann

    2005-04-01

    Juvenile hormone (JH) is necessary for the production of vitellogenin (Vg) in the boll weevil, Anthonomus grandis. Occurrence of Vg in this species is typically restricted to reproductively competent females, and is not detected in untreated males. However, the JH analog, methoprene stimulates Vg production in intact males and in the isolated abdomens of both male and female boll weevils (where in each case no Vg is detected without treatment), suggesting that males are competent to produce Vg but are normally not stimulated to do so. Preliminary work indicating that male boll weevil corpora allata (CA) produced little or no JH in vitro suggested that failure of males to produce Vg might be due to very low JH levels compared to females. This study re-examines the question of JH in male boll weevils by determining in vitro production of JH III by male CA during the first 10 days after adult emergence, determining hemolymph JH esterase activity during this same time period and hemolymph JH III titers in adults of both sexes. We also re-examine the ability of isolated male abdomens to produce Vg in response to hormonal stimulation, analyzing the effect of a wide range of methoprene and JH III dosages. Results indicate that male A. grandis have circulating JH titers and JH production similar to females. JH esterase activity is slightly but significantly higher in males than females. Vg production by isolated abdomens of both sexes after stimulation with methoprene or JH III was confirmed. Dose response studies indicated that high levels of methoprene were less effective than intermediate doses in stimulating Vg synthesis in both sexes. We conclude that the sexually dimorphic effect of JH on Vg synthesis is not due to differences in JH production or differences in JH titer between the sexes.

  8. Wnt Signaling Cross-Talks with JH Signaling by Suppressing Met and gce Expression

    PubMed Central

    Abdou, Mohamed; Peng, Cheng; Huang, Jianhua; Zyaan, Ola; Wang, Sheng; Li, Sheng; Wang, Jian

    2011-01-01

    Juvenile hormone (JH) plays key roles in controlling insect growth and metamorphosis. However, relatively little is known about the JH signaling pathways. Until recent years, increasing evidence has suggested that JH modulates the action of 20-hydroxyecdysone (20E) by regulating expression of broad (br), a 20E early response gene, through Met/Gce and Kr-h1. To identify other genes involved in JH signaling, we designed a novel Drosophila genetic screen to isolate mutations that derepress JH-mediated br suppression at early larval stages. We found that mutations in three Wnt signaling negative regulators in Drosophila, Axin (Axn), supernumerary limbs (slmb), and naked cuticle (nkd), caused precocious br expression, which could not be blocked by exogenous JHA. A similar phenotype was observed when armadillo (arm), the mediator of Wnt signaling, was overexpressed. qRT-PCR revealed that Met, gce and Kr-h1expression was suppressed in the Axn, slmb and nkd mutants as well as in arm gain-of-function larvae. Furthermore, ectopic expression of gce restored Kr-h1 expression but not Met expression in the arm gain-of-function larvae. Taken together, we conclude that Wnt signaling cross-talks with JH signaling by suppressing transcription of Met and gce, genes that encode for putative JH receptors. The reduced JH activity further induces down-regulation of Kr-h1expression and eventually derepresses br expression in the Drosophila early larval stages. PMID:22087234

  9. Involvement of Met and Kr-h1 in JH-Mediated Reproduction of Female Bactrocera dorsalis (Hendel).

    PubMed

    Yue, Yong; Yang, Rui-Lin; Wang, Wei-Ping; Zhou, Qi-Hao; Chen, Er-Hu; Yuan, Guo-Rui; Wang, Jin-Jun; Dou, Wei

    2018-01-01

    Juvenile hormone (JH) prevents metamorphosis during insect larval stages and promotes adult reproductive processes. Krüppel-homolog 1 (Kr-h1), a zinc finger transcription factor assumed to be induced by JH via the JH receptor methoprene-tolerant (Met), mediates the antimetamorphic effect of JH in insects, but its function in JH-mediated reproductive processes has not been fully explored. In this study, Met and Kr-h1 involved in the JH signaling pathway were first cloned and identified from the oriental fruit fly, Bactrocera dorsalis , an important pest infesting fruit and vegetables worldwide. Subsequent spatiotemporal expression analysis revealed that Met and Kr-h1 were both highly expressed in 7-day-old adults and fat body of female adults, respectively. Treatment with a JH analog (methoprene) significantly induced the expression of JH signaling and vitellogenin ( Vg ) genes and accelerated ovary development. RNA interference (RNAi) further revealed that either Met or Kr-h1 depletion at the adult stage of B. dorsalis impeded ovary development, with significantly lower egg production noted as well. In addition, rescue through methoprene application after RNAi stimulated the expression of JH signaling and Vg genes. Although there were still differences in ovary phenotype between rescued insects and the pre-RNAi control, ovary redevelopment with a larger surface area was observed, consistent with the spatiotemporal expression and phenotypes recorded in the original methoprene experiment. Our data reveal the involvement of Met and Kr-h1 in insect vitellogenesis and egg production, thus indicating the crucial role of the JH signaling pathway in insect reproduction.

  10. Purification of astaxanthin from mutant of Phaffia rhodozyma JH-82 which isolated from forests trees of Iran.

    PubMed

    Golkhoo, Shokufeh; Barantalab, Fatemeh; Ahmadi, Ali Reza; Hassan, Zuhair Muhammad

    2007-03-01

    Astaxanthin have been extracted and purified from mutant isolate of Phaffia rhodozyma JH-82. Purified astaxanthin was identified by spectrophotometric, TLC and HPLC analysis and were compared with synthetic astaxanthin. Results of TLC analysis indicated that isolate of P. rhodozyma JH-82 were able to produce nine different carotenoids and high level of carotenoids was belong to astaxanthin. Results of this study for pure astaxanthin production indicated that mutant of JH-82 of P. rhodozyma (230 microg g(-1)(dried yeast)) produced more astaxanthin than natural isolate JH-80 (140 microg g(-1)(dried yeast)). The HPLC spectrum showed retention time 11 min for both purified and synthetic astaxanthin and solvent was CDCl3.

  11. A novel mutation in the JH4 domain of JAK3 causing severe combined immunodeficiency complicated by vertebral osteomyelitis.

    PubMed

    Qamar, Farah; Junejo, Samina; Qureshi, Sonia; Seleman, Michael; Bainter, Wayne; Massaad, Michel; Chou, Janet; Geha, Raif S

    2017-10-01

    JAK3 is a tyrosine kinase essential for signaling downstream of the common gamma chain subunit shared by multiple cytokine receptors. JAK3 deficiency results in T - B + NK - severe combined immune deficiency (SCID). We report a patient with SCID due to a novel mutation in the JAK3 JH4 domain. The function of the JH4 domain remains unknown. This is the first report of a missense mutation in the JAK3 JH4 domain, thereby demonstrating the importance of the JH4 domain of JAK3 in host immunity. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Effects of protectant and rehydration conditions on the survival rate and malolactic fermentation efficiency of freeze-dried Lactobacillus plantarum JH287.

    PubMed

    Lee, Sae-Byuk; Kim, Dong-Hwan; Park, Heui-Dong

    2016-09-01

    In this study, Lactobacillus plantarum JH287 was used as a malolactic fermentation starter in Campbell Early wine production. L. plantarum JH287 was first lyophilized, and the malolactic fermentation potential of freeze-dried L. plantarum JH287 was investigated. Different protective media and rehydration conditions were tested to improve the survival rate of freeze-dried L. plantarum JH287. Optimal protective medium contained 10 % sorbitol and 10 % skim milk. The optimal rehydration condition was a 1-h rehydration time conducted in the same protective media, and the combination of these two methods produced a survival rate of 86.37 %. In addition, a 77.71 % survival rate was achieved using freeze-dried samples that were stored at 4 °C for 2 months. Freeze-dried L. plantarum JH287 and Saccharomyces cerevisiae Fermivin were used to inoculate the Campbell Early grape must to decrease its malic acid content. Using this mixed-fermentation method, wine showed a decrease in malic acid content after 9 days of fermentation. GC-MS analysis detected 15 volatile ester compounds in the wine. A sensory evaluation showed that the taste and aroma of mix-fermented wine were better than those of the control that had not been inoculated with L. plantarum JH287.

  13. JAK2 JH2 Fluorescence Polarization Assay and Crystal Structures for Complexes with Three Small Molecules.

    PubMed

    Newton, Ana S; Deiana, Luca; Puleo, David E; Cisneros, José A; Cutrona, Kara J; Schlessinger, Joseph; Jorgensen, William L

    2017-06-08

    A competitive fluorescence polarization (FP) assay is reported for determining binding affinities of probe molecules with the pseudokinase JAK2 JH2 allosteric site. The syntheses of the fluorescent 5 and 6 used in the assay are reported as well as K d results for 10 compounds, including JNJ7706621, NVP-BSK805, and filgotinib (GLPG0634). X-ray crystal structures of JAK2 JH2 in complex with NVP-BSK805, filgotinib, and diaminopyrimidine 8 elucidate the binding poses.

  14. JAK2 JH2 Fluorescence Polarization Assay and Crystal Structures for Complexes with Three Small Molecules

    PubMed Central

    2017-01-01

    A competitive fluorescence polarization (FP) assay is reported for determining binding affinities of probe molecules with the pseudokinase JAK2 JH2 allosteric site. The syntheses of the fluorescent 5 and 6 used in the assay are reported as well as Kd results for 10 compounds, including JNJ7706621, NVP-BSK805, and filgotinib (GLPG0634). X-ray crystal structures of JAK2 JH2 in complex with NVP-BSK805, filgotinib, and diaminopyrimidine 8 elucidate the binding poses. PMID:28626520

  15. Use of JH4 joining segment gene by an anti-arsonate antibody that bears the major A-strain cross-reactive idiotype but displays diminished antigen binding.

    PubMed

    Slaughter, C A; Jeske, D J; Kuziel, W A; Milner, E C; Capra, J D

    1984-06-01

    One of the antibody families utilized by the A/J mouse in its response to p-azophenylarsonate (Ars) is characterized by the expression of the major anti-arsonate cross-reactive idiotype (CRI) of the A strain. This family has been termed the Ars-A family. A hybridoma antibody (HP 101F11 ) obtained after immunization of an A/J mouse with Ars was identified initially as displaying the CRI, but was subsequently found to bind antigen at a level much lower than most members of the Ars-A family. The results of binding studies suggested that HP 101F11 possesses reduced avidity for antigen. When isolated light and heavy chains were allowed to recombine with the heavy and light chains of a strongly antigen-binding, strongly CRI-positive antibody of the Ars-A family (HP 93G7 ), the low level of antigen binding by HP 101F11 was found to be due to a structurally variant heavy chain. Whereas antibodies of the Ars-A family with normal avidity for antigen had been shown to use the JH2 joining segment gene, amino acid sequence analysis of HP 101F11 revealed that this antibody has a JH segment with a sequence identical to that encoded by a portion of a different JH gene, JH4 . The implication that 101F11 uses the JH4 gene instead of JH2 was supported by the observation that the productively rearranged gene is associated with an Eco R1 restriction fragment 0.95 Kb smaller than the corresponding fragments of Ars-A hybridomas with normal avidity for antigen. The size difference of 0.95 Kb corresponds exactly to the known distance between the JH2 and JH4 genes in BALB/c germline DNA. In addition to the structural differences immediately attributable to the use of JH4 , HP 101F11 has shown an amino acid interchange in the DH segment, and a single amino acid deletion at the DH-JH boundary. These results show that variation among members of the Ars-A family in the DH and/or JH segments provides alternative structural forms of Ars-A antibodies upon which selective processes can operate

  16. Crystal Structure of Silkworm Bombyx mori JHBP in Complex With 2-Methyl-2,4-Pentanediol: Plasticity of JH-Binding Pocket and Ligand-Induced Conformational Change of the Second Cavity in JHBP

    PubMed Central

    Fujimoto, Zui; Suzuki, Rintaro; Shiotsuki, Takahiro; Tsuchiya, Wataru; Tase, Akira; Momma, Mitsuru; Yamazaki, Toshimasa

    2013-01-01

    Juvenile hormones (JHs) control a diversity of crucial life events in insects. In Lepidoptera which major agricultural pests belong to, JH signaling is critically controlled by a species-specific high-affinity, low molecular weight JH-binding protein (JHBP) in hemolymph, which transports JH from the site of its synthesis to target tissues. Hence, JHBP is expected to be an excellent target for the development of novel specific insect growth regulators (IGRs) and insecticides. A better understanding of the structural biology of JHBP should pave the way for the structure-based drug design of such compounds. Here, we report the crystal structure of the silkworm Bombyx mori JHBP in complex with two molecules of 2-methyl-2,4-pentanediol (MPD), one molecule (MPD1) bound in the JH-binding pocket while the other (MPD2) in a second cavity. Detailed comparison with the apo-JHBP and JHBP-JH II complex structures previously reported by us led to a number of intriguing findings. First, the JH-binding pocket changes its size in a ligand-dependent manner due to flexibility of the gate α1 helix. Second, MPD1 mimics interactions of the epoxide moiety of JH previously observed in the JHBP-JH complex, and MPD can compete with JH in binding to the JH-binding pocket. We also confirmed that methoprene, which has an MPD-like structure, inhibits the complex formation between JHBP and JH while the unepoxydated JH III (methyl farnesoate) does not. These findings may open the door to the development of novel IGRs targeted against JHBP. Third, binding of MPD to the second cavity of JHBP induces significant conformational changes accompanied with a cavity expansion. This finding, together with MPD2-JHBP interaction mechanism identified in the JHBP-MPD complex, should provide important guidance in the search for the natural ligand of the second cavity. PMID:23437107

  17. Insight into resistance mechanism of anaplastic lymphoma kinase to alectinib and JH-VIII-157-02 caused by G1202R solvent front mutation.

    PubMed

    Wang, Han; Wang, Yao; Guo, Wentao; Du, Bin; Huang, Xiaobing; Wu, Riping; Yang, Baoyu; Lin, Xiaoyan; Wu, Yilan

    2018-01-01

    Mutated anaplastic lymphoma kinase (ALK) drives the development of advanced non-small cell lung cancer (NSCLC). Most reported small-molecule inhibitors targeting the ALK domain do not display good inhibition of the G1202R solvent front mutation. The solvent front mutation was assumed to hinder drug binding. However, a different fact could be uncovered by the simulations reported in this study through a structural analog of alectinib (JH-VIII-157-02), which demonstrated potent effects against the G1202R mutation. Molecular docking, conventional molecular dynamics (MD) simulations, free energy calculations, and umbrella sampling (US) simulations were carried out to make clear the principles of the binding preferences of alectinib and JH-VIII-157-02 toward ALK WT and the ALK G1202R (ALK G1202R ) mutation. JH-VIII-157-02 has similar binding affinities to both ALK WT and ALK G1202R whereas it has has a much lower binding affinity for alectinib to ALK G1202R . Analysis of individual energy terms indicate the major variation involves the van der Waals and entropy terms. Structural analysis reveals that the conformational change of the ATP-binding glycine-rich loop was primarily responsible for the alectinib resistance, not JH-VIII-157-02. In addition, US simulations prove JH-VIII-157-02 has similar dissociative processes from both ALK WT and ALK G1202R , while alectinib is more easily dissociated from ALK G1202R than from ALK WT , thus indicating lesser residence time. Both the binding affinity and the drug residence time should be emphasized in rational drug design to overcome the G1202R solvent front mutation in ALK resistance.

  18. Insight into resistance mechanism of anaplastic lymphoma kinase to alectinib and JH-VIII-157-02 caused by G1202R solvent front mutation

    PubMed Central

    Wang, Han; Wang, Yao; Guo, Wentao; Du, Bin; Huang, Xiaobing; Wu, Riping; Yang, Baoyu; Lin, Xiaoyan; Wu, Yilan

    2018-01-01

    Background Mutated anaplastic lymphoma kinase (ALK) drives the development of advanced non-small cell lung cancer (NSCLC). Most reported small-molecule inhibitors targeting the ALK domain do not display good inhibition of the G1202R solvent front mutation. The solvent front mutation was assumed to hinder drug binding. However, a different fact could be uncovered by the simulations reported in this study through a structural analog of alectinib (JH-VIII-157-02), which demonstrated potent effects against the G1202R mutation. Methods Molecular docking, conventional molecular dynamics (MD) simulations, free energy calculations, and umbrella sampling (US) simulations were carried out to make clear the principles of the binding preferences of alectinib and JH-VIII-157-02 toward ALKWT and the ALK G1202R (ALKG1202R) mutation. Results JH-VIII-157-02 has similar binding affinities to both ALKWT and ALKG1202R whereas it has has a much lower binding affinity for alectinib to ALKG1202R. Analysis of individual energy terms indicate the major variation involves the van der Waals and entropy terms. Structural analysis reveals that the conformational change of the ATP-binding glycine-rich loop was primarily responsible for the alectinib resistance, not JH-VIII-157-02. In addition, US simulations prove JH-VIII-157-02 has similar dissociative processes from both ALKWT and ALKG1202R, while alectinib is more easily dissociated from ALKG1202R than from ALKWT, thus indicating lesser residence time. Conclusion Both the binding affinity and the drug residence time should be emphasized in rational drug design to overcome the G1202R solvent front mutation in ALK resistance. PMID:29785088

  19. Exogenous JH and ecdysteroid applications alter initiation of polydnaviral replication in an endoparasitoid wasp, Cotesia plutellae (Braconidae: Hymenoptera).

    PubMed

    Park, Bokri; Kim, Yonggyun

    2011-06-01

    Polydnaviruses are a group of double-stranded DNA viruses and are symbiotically associated with some ichneumonoid wasps. As proviruses, the replication of polydnaviruses occurs in the female reproductive organ at the pupal stage. This study analyzed the effects of two developmental hormones, juvenile hormone (JH) and ecdysteroid, on the viral replication of Cotesia plutellae bracovirus (CpBV). All 23 CpBV segments identified contained a conserved excision/rejoining site ('AGCTTT') from their proviral segments. Using quantitative real-time PCR based on this excision/rejoining site marker, initiation of CpBV replication was determined to have occurred on day 4 on the pupal stage. Pyriproxyfen, a JH agonist, significantly inhibited adult emergence of C. plutellae, whereas RH5992, an ecdysteroid agonist, had no inhibitory effect. Although RH5992 had no effect dose on adult development, it significantly accelerated viral replication. The results of immunoblotting assays against viral coat proteins support the effects of the hormone agonists on viral replication.

  20. Enhancement of D-lactic acid production from a mixed glucose and xylose substrate by the Escherichia coli strain JH15 devoid of the glucose effect.

    PubMed

    Lu, Hongying; Zhao, Xiao; Wang, Yongze; Ding, Xiaoren; Wang, Jinhua; Garza, Erin; Manow, Ryan; Iverson, Andrew; Zhou, Shengde

    2016-02-19

    A thermal tolerant stereo-complex poly-lactic acid (SC-PLA) can be made by mixing Poly-D-lactic acid (PDLA) and poly-L-lactic acid (PLLA) at a defined ratio. This environmentally friendly biodegradable polymer could replace traditional recalcitrant petroleum-based plastics. To achieve this goal, however, it is imperative to produce optically pure lactic acid isomers using a cost-effective substrate such as cellulosic biomass. The roadblock of this process is that: 1) xylose derived from cellulosic biomass is un-fermentable by most lactic acid bacteria; 2) the glucose effect results in delayed and incomplete xylose fermentation. An alternative strain devoid of the glucose effect is needed to co-utilize both glucose and xylose for improved D-lactic acid production using a cellulosic biomass substrate. A previously engineered L-lactic acid Escherichia coli strain, WL204 (ΔfrdBC ΔldhA ΔackA ΔpflB ΔpdhR ::pflBp6-acEF-lpd ΔmgsA ΔadhE, ΔldhA::ldhL), was reengineered for production of D-lactic acid, by replacing the recombinant L-lactate dehydrogenase gene (ldhL) with a D-lactate dehydrogenase gene (ldhA). The glucose effect (catabolite repression) of the resulting strain, JH13, was eliminated by deletion of the ptsG gene which encodes for IIBC(glc) (a PTS enzyme for glucose transport). The derived strain, JH14, was metabolically evolved through serial transfers in screw-cap tubes containing glucose. The evolved strain, JH15, regained improved anaerobic cell growth using glucose. In fermentations using a mixture of glucose (50 g L(-1)) and xylose (50 g L(-1)), JH15 co-utilized both glucose and xylose, achieving an average sugar consumption rate of 1.04 g L(-1)h(-1), a D-lactic acid titer of 83 g L(-1), and a productivity of 0.86 g L(-1) h(-1). This result represents a 46 % improved sugar consumption rate, a 26 % increased D-lactic acid titer, and a 48 % enhanced productivity, compared to that achieved by JH13. These results demonstrated that JH15 has

  1. Contribution of the autolysin AtlA to the bactericidal activity of amoxicillin against Enterococcus faecalis JH2-2.

    PubMed

    Bravetti, Anne-Lise; Mesnage, Stéphane; Lefort, Agnès; Chau, Françoise; Eckert, Catherine; Garry, Louis; Arthur, Michel; Fantin, Bruno

    2009-04-01

    The bactericidal activity of amoxicillin was investigated against Enterococcus faecalis JH2-2 and against an isogenic mutant deficient in the production of the N-acetylglucosaminidase AtlA. Comparison of the two strains indicated that this autolysin contributes to killing by amoxicillin both in vitro and in a rabbit model of experimental endocarditis.

  2. Tyrosine phosphorylation of Jak2 in the JH2 domain inhibits cytokine signaling.

    PubMed

    Feener, Edward P; Rosario, Felicia; Dunn, Sarah L; Stancheva, Zlatina; Myers, Martin G

    2004-06-01

    Jak family tyrosine kinases mediate signaling by cytokine receptors to regulate diverse biological processes. Although Jak2 and other Jak kinase family members are phosphorylated on numerous sites during cytokine signaling, the identity and function of most of these sites remains unknown. Using tandem mass spectroscopic analysis of activated Jak2 protein from intact cells, we identified Tyr(221) and Tyr(570) as novel sites of Jak2 phosphorylation. Phosphorylation of both sites was stimulated by cytokine treatment of cultured cells, and this stimulation required Jak2 kinase activity. While we observed no gross alteration of signaling upon mutation of Tyr(221), Tyr(570) lies within the inhibitory JH2 domain of Jak2, and mutation of this site (Jak2(Y570F)) results in constitutive Jak2-dependent signaling in the absence of cytokine stimulation and enhances and prolongs Jak2 activation during cytokine stimulation. Mutation of Tyr(570) does not alter the ability of SOCS3 to bind or inhibit Jak2, however. Thus, the phosphorylation of Tyr(570) in vivo inhibits Jak2-dependent signaling independently of SOCS3-mediated inhibition. This Tyr(570)-dependent mechanism of Jak2 inhibition likely represents an important mechanism by which cytokine function is regulated.

  3. Modulation of calcium carbonate precipitation by exopolysaccharide in Bacillus sp. JH7.

    PubMed

    Kim, Hyun Jung; Shin, Bora; Lee, Yun Suk; Park, Woojun

    2017-08-01

    Extracellular polymeric substance (EPS) is proposed to facilitate calcium ion supersaturation through its nucleation effect during the microbially induced calcium carbonate precipitation (MICP) process. However, the supersaturation effect of Ca 2+ via EPS in MICP has not been clearly demonstrated. Enhanced exopolysaccharide production of the alkali- and halotolerant MICP-capable bacteria, Bacillus sp. JH7, was achieved through glycerol addition. This was demonstrated by measuring cellular precipitation and Congo red binding. Interestingly, field emission scanning electron microscopy and energy-dispersive X-ray spectrometry analysis demonstrated that there was no MICP under glycerol-amended conditions. Although glycerol promoted exopolysaccharide capture of Ca 2+ ions, Ca 2+ embedded onto EPS did not participate in MICP formation. The pH was reduced in glycerol-added media, which led us to analyze high acetate production under our test conditions. Purified glycerol-induced exopolysaccharide showed a higher capacity of Ca 2+ capture than the control. Quantitative RT-PCR analysis showed that three genes involved in exopolysaccharide production were highly upregulated by glycerol. The amounts of three detected monosaccharides (arabinose, glucose, and mannose) were altered by glycerol. Cell hydrophobicity measurements indicated that glycerol could confer more hydrophilic characteristics to cells, which might enhance Ca 2+ binding onto EPS. Unexpectedly, our data demonstrated, for the first time, that glycerol could promote exopolysaccharide and acetate production under our test condition, which could inhibit MICP by reducing the availability of free Ca 2+ .

  4. Rapid measurement of 3J(H N-H alpha) and 3J(N-H beta) coupling constants in polypeptides.

    PubMed

    Barnwal, Ravi Pratap; Rout, Ashok K; Chary, Kandala V R; Atreya, Hanudatta S

    2007-12-01

    We present two NMR experiments, (3,2)D HNHA and (3,2)D HNHB, for rapid and accurate measurement of 3J(H N-H alpha) and 3J(N-H beta) coupling constants in polypeptides based on the principle of G-matrix Fourier transform NMR spectroscopy and quantitative J-correlation. These experiments, which facilitate fast acquisition of three-dimensional data with high spectral/digital resolution and chemical shift dispersion, will provide renewed opportunities to utilize them for sequence specific resonance assignments, estimation/characterization of secondary structure with/without prior knowledge of resonance assignments, stereospecific assignment of prochiral groups and 3D structure determination, refinement and validation. Taken together, these experiments have a wide range of applications from structural genomics projects to studying structure and folding in polypeptides.

  5. Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.

    PubMed

    He, Mingyue; Hamon, Maureen; Liu, Hong; Corper, Adam L; Taussig, Michael J

    2006-09-01

    The crystal structures of the Fab' fragment of the anti-progesterone monoclonal antibody DB3 and its complexes with steroid haptens have shown that the D-JH junctional residue TrpH100 is a key contributor to binding site interactions with ligands. The indole group of TrpH100 also undergoes a significant conformational change between the bound and unliganded states, effectively opening and closing the combining site pocket. In order to explore the effect of substitutions at this position on steroid recognition, we have carried out mutagenesis on a construct encoding a three-domain single-chain fragment (VH/K) of DB3 expressed in Escherichia coli. TrpH100 was replaced by 13 different amino acids or deleted, and the functional and antigenic properties of the mutated fragments were analyzed. Most substitutions, including small, hydrophobic, hydrophilic, neutral, and negatively charged side chains, were reduced or abolished binding to free progesterone, although binding to progesterone-BSA was partially retained. The reduction in antigen binding was paralleled by alteration of the idiotype associated with the DB3 combining site. In contrast, the replacement of TrpH100 by Arg produced a mutant that retained wild-type antibody affinity and idiotype, but with altered specificity. Significant changes in this mutant included increased relative affinities of 10(4)-fold for progesterone-3-carboxymethyloxime and 10-fold for aetiocholanolone. Our results demonstrate an essential role for the junctional residue H100 in determining steroid-binding specificity and combining site idiotype and show that these properties can be changed by a single amino acid substitution at this position.

  6. Preemptive Approach to Improving Survival in Epithelial Ovarian Cancer

    DTIC Science & Technology

    2012-06-01

    Metab 2008;93:3471–7. 692[87] Cho JH, Lee JG, Yang YI, Kim JH, Ahn JH, Baek NI, Lee KT, Choi JH. Eupatilin, a die- 693tary flavonoid , induces G2/M cell...Metab 2008;93:3471–7. 692[87] Cho JH, Lee JG, Yang YI, Kim JH, Ahn JH, Baek NI, Lee KT, Choi JH. Eupatilin, a die- 693tary flavonoid , induces G2/M

  7. Juvenile Hormone Biosynthesis Gene Expression in the corpora allata of Honey Bee (Apis mellifera L.) Female Castes

    PubMed Central

    Rosa, Gustavo Conrado Couto; Moda, Livia Maria; Martins, Juliana Ramos; Bitondi, Márcia Maria Gentile; Hartfelder, Klaus; Simões, Zilá Luz Paulino

    2014-01-01

    Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showing relatively high transcript abundance was contrasted with CA size, hemolymph JH titer, as well as JH degradation rates and JH esterase (jhe) transcript levels. Gene expression did not match the contrasting JH titers in queen and worker fourth instar larvae, but jhe transcript abundance and JH degradation rates were significantly lower in queen larvae. Consequently, transcriptional control of JHE is of importance in regulating larval JH titers and caste development. In contrast, the same analyses applied to adult worker bees allowed us inferring that the high JH levels in foragers are due to increased JH synthesis. Upon RNAi-mediated silencing of the methyl farnesoate epoxidase gene (mfe) encoding the enzyme that catalyzes methyl farnesoate-to-JH conversion, the JH titer was decreased, thus corroborating that JH titer regulation in adult honey bees depends on this final JH biosynthesis step. The molecular pathway differences underlying JH titer regulation in larval caste development versus adult age polyethism lead us to propose that mfe and jhe genes be assayed when addressing questions on the role(s) of JH in social evolution. PMID:24489805

  8. Effects of juvenile hormone and its analogs on vitellogenin synthesis and ovarian development in Ornithodoros moubata (Acari: Argasidae).

    PubMed

    Chinzei, Y; Taylor, D; Ando, K

    1991-07-01

    Effects of juvenile hormones (JH) and JH analogs on the release of vitellogenin (Vg) into the hemolymph and ovarian development in unfed adult female ticks, Ornithodoros moubata (Murray), were investigated. Topical application of acetone solvent and injection of acetone or oils showed some increase in Vg titer in the hemolymph. Topical application of JH (JH I, JH II, JH III) and JH analogs (methoprene, S21149, S21150, and S31183) dissolved in acetone to unengorged adult females elevated Vg titer in the hemolymph but only to the same level as the acetone controls. These effects were independent of dose. Injection of JH (JH I, JH II, JH III) and methoprene dissolved in mineral oil also did not significantly increase the Vg titer in the hemolymph compared with the controls (mineral oil injection). Electrophoretic analysis of hemolymph from females 5 d after treatment topically or by injection with JH and JH analogs showed faint Vg bands which comigrated with Vg's (Vg-1 and Vg-2) of normal, engorged female hemolymph, but Vg bands were detected more clearly in the hemolymph samples that were collected greater than 2 wk after treatment. However, the same level of Vg also was detected in the hemolymph of females treated with acetone or mineral oil. Vg synthesis in females treated with JH and JH analogs was analyzed by in vivo labeling and fluorography, which showed that Vg synthesis was not induced by application of JH to unengorged females.

  9. Characterization of the Drosophila Methoprene -tolerant gene product. Juvenile hormone binding and ligand-dependent gene regulation.

    PubMed

    Miura, Ken; Oda, Masahito; Makita, Sumiko; Chinzei, Yasuo

    2005-03-01

    Juvenile hormones (JHs) of insects are sesquiterpenoids that regulate a great diversity of processes in development and reproduction. As yet the molecular modes of action of JH are poorly understood. The Methoprene-tolerant (Met) gene of Drosophila melanogaster has been found to be responsible for resistance to a JH analogue (JHA) insecticide, methoprene. Previous studies on Met have implicated its involvement in JH signaling, although direct evidence is lacking. We have now examined the product of Met (MET) in terms of its binding to JH and ligand-dependent gene regulation. In vitro synthesized MET directly bound to JH III with high affinity (Kd = 5.3 +/- 1.5 nm, mean +/- SD), consistent with the physiological JH concentration. In transient transfection assays using Drosophila S2 cells the yeast GAL4-DNA binding domain fused to MET exerted JH- or JHA-dependent activation of a reporter gene. Activation of the reporter gene was highly JH- or JHA-specific with the order of effectiveness: JH III > JH II > JH I > methoprene; compounds which are only structurally related to JH or JHA did not induce any activation. Localization of MET in the S2 cells was nuclear irrespective of the presence or absence of JH. These results suggest that MET may function as a JH-dependent transcription factor.

  10. SCExAO and GPI Y JH band photometry and integral field spectroscopy of the young brown dwarf companion to HD 1160

    DOE PAGES

    Garcia, Eugenio Victor; Currie, Thayne; Guyon, Olivier; ...

    2017-01-10

    Here, we present high signal-to-noise ratio, precise Y JH photometry and Y band (0.957–1.120 μm) spectroscopy of HD 1160 B, a young substellar companion discovered from the Gemini NICI Planet Finding Campaign using the Subaru Coronagraphic Extreme Adaptive Optics instrument and the Gemini Planet Imager. HD 1160 B has typical mid-M dwarf-like infrared colors and a spectral type of M5.5more » $${}_{-0.5}^{+1.0}$$, where the blue edge of our Y band spectrum rules out earlier spectral types. Atmospheric modeling suggests HD 1160 B has an effective temperature of 3000–3100 K, a surface gravity of log g = 4–4.5, a radius of 1.55 ± 0.10 R J, and a luminosity of log L/L ⊙ = –2.76 ± 0.05. Neither the primary's Hertzspring–Russell diagram position nor atmospheric modeling of HD 1160 B show evidence for a subsolar metallicity. Interpretation of the HD 1160 B spectroscopy depends on which stellar system components are used to estimate the age. Considering HD 1160 A, B and C jointly, we derive an age of 80–125 Myr, implying that HD 1160 B straddles the hydrogen-burning limit (70–90 M J). If we consider HD 1160 A alone, younger ages (20–125 Myr) and a brown dwarf-like mass (35–90 M J) are possible. Interferometric measurements of the primary, a precise Gaia parallax, and moderate-resolution spectroscopy can better constrain the system's age and how HD 1160 B fits within the context of (sub)stellar evolution.« less

  11. SCExAO and GPI Y JH band photometry and integral field spectroscopy of the young brown dwarf companion to HD 1160

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Garcia, Eugenio Victor; Currie, Thayne; Guyon, Olivier

    Here, we present high signal-to-noise ratio, precise Y JH photometry and Y band (0.957–1.120 μm) spectroscopy of HD 1160 B, a young substellar companion discovered from the Gemini NICI Planet Finding Campaign using the Subaru Coronagraphic Extreme Adaptive Optics instrument and the Gemini Planet Imager. HD 1160 B has typical mid-M dwarf-like infrared colors and a spectral type of M5.5more » $${}_{-0.5}^{+1.0}$$, where the blue edge of our Y band spectrum rules out earlier spectral types. Atmospheric modeling suggests HD 1160 B has an effective temperature of 3000–3100 K, a surface gravity of log g = 4–4.5, a radius of 1.55 ± 0.10 R J, and a luminosity of log L/L ⊙ = –2.76 ± 0.05. Neither the primary's Hertzspring–Russell diagram position nor atmospheric modeling of HD 1160 B show evidence for a subsolar metallicity. Interpretation of the HD 1160 B spectroscopy depends on which stellar system components are used to estimate the age. Considering HD 1160 A, B and C jointly, we derive an age of 80–125 Myr, implying that HD 1160 B straddles the hydrogen-burning limit (70–90 M J). If we consider HD 1160 A alone, younger ages (20–125 Myr) and a brown dwarf-like mass (35–90 M J) are possible. Interferometric measurements of the primary, a precise Gaia parallax, and moderate-resolution spectroscopy can better constrain the system's age and how HD 1160 B fits within the context of (sub)stellar evolution.« less

  12. Jungle Honey Enhances Immune Function and Antitumor Activity

    PubMed Central

    Fukuda, Miki; Kobayashi, Kengo; Hirono, Yuriko; Miyagawa, Mayuko; Ishida, Takahiro; Ejiogu, Emenike C.; Sawai, Masaharu; Pinkerton, Kent E.; Takeuchi, Minoru

    2011-01-01

    Jungle honey (JH) is collected from timber and blossom by wild honey bees that live in the tropical forest of Nigeria. JH is used as a traditional medicine for colds, skin inflammation and burn wounds as well as general health care. However, the effects of JH on immune functions are not clearly known. Therefore, we investigated the effects of JH on immune functions and antitumor activity in mice. Female C57BL/6 mice were injected with JH (1 mg/mouse/day, seven times intra-peritoneal). After seven injections, peritoneal cells (PC) were obtained. Antitumor activity was assessed by growth of Lewis Lung Carcinoma/2 (LL/2) cells. PC numbers were increased in JH-injected mice compared to control mice. In Dot Plot analysis by FACS, a new cell population appeared in JH-injected mice. The percent of Gr-1 surface antigen and the intensity of Gr-1 antigen expression of PC were increased in JH-injected mice. The new cell population was neutrophils. JH possessed chemotactic activity for neutrophils. Tumor incidence and weight were decreased in JH-injected mice. The ratio of reactive oxygen species (ROS) producing cells was increased in JH-injected mice. The effective component in JH was fractionized by gel filtration using HPLC and had an approximate molecular weight (MW) of 261. These results suggest that neutrophils induced by JH possess potent antitumor activity mediated by ROS and the effective immune component of JH is substrate of MW 261. PMID:19141489

  13. Genome-wide comparison of genes involved in the biosynthesis, metabolism, and signaling of juvenile hormone between silkworm and other insects

    PubMed Central

    Cheng, Daojun; Meng, Meng; Peng, Jian; Qian, Wenliang; Kang, Lixia; Xia, Qingyou

    2014-01-01

    Juvenile hormone (JH) contributes to the regulation of larval molting and metamorphosis in insects. Herein, we comprehensively identified 55 genes involved in JH biosynthesis, metabolism and signaling in the silkworm (Bombyx mori) as well as 35 in Drosophila melanogaster, 35 in Anopheles gambiae, 36 in Apis mellifera, 47 in Tribolium castaneum, and 44 in Danaus plexippus. Comparative analysis showed that each gene involved in the early steps of the mevalonate (MVA) pathway, in the neuropeptide regulation of JH biosynthesis, or in JH signaling is a single copy in B. mori and other surveyed insects, indicating that these JH-related pathways or steps are likely conserved in all surveyed insects. However, each gene participating in the isoprenoid branch of JH biosynthesis and JH metabolism, together with the FPPS genes for catalyzing the final step of the MVA pathway of JH biosynthesis, exhibited an obvious duplication in Lepidoptera, including B. mori and D. plexippus. Microarray and real-time RT-PCR analysis revealed that different copies of several JH-related genes presented expression changes that correlated with the dynamics of JH titer during larval growth and metamorphosis. Taken together, the findings suggest that duplication-derived copy variation of JH-related genes might be evolutionarily associated with the variation of JH types between Lepidoptera and other insect orders. In conclusion, our results provide useful clues for further functional analysis of JH-related genes in B. mori and other insects. PMID:25071411

  14. Heat Shock Protein 83 (Hsp83) Facilitates Methoprene-tolerant (Met) Nuclear Import to Modulate Juvenile Hormone Signaling*

    PubMed Central

    He, Qianyu; Wen, Di; Jia, Qiangqiang; Cui, Chunlai; Wang, Jian; Palli, Subba R.; Li, Sheng

    2014-01-01

    Juvenile hormone (JH) receptors, methoprene-tolerant (Met) and Germ-cell expressed (Gce), transduce JH signals to induce Kr-h1 expression in Drosophila. Dual luciferase assay identified a 120-bp JH response region (JHRR) in the Kr-h1α promoter. Both in vitro and in vivo experiments revealed that Met and Gce transduce JH signals to induce Kr-h1 expression through the JHRR. DNA affinity purification identified chaperone protein Hsp83 as one of the proteins bound to the JHRR in the presence of JH. Interestingly, Hsp83 physically interacts with PAS-B and basic helix-loop-helix domains of Met, and JH induces Met-Hsp83 interaction. As determined by immunohistochemistry, Met is mainly distributed in the cytoplasm of fat body cells of the larval when the JH titer is low and JH induces Met nuclear import. Hsp83 was accumulated in the cytoplasm area adjunct to the nucleus in the presence of JH and Met/Gce. Loss-of-function of Hsp83 attenuated JH binding and JH-induced nuclear import of Met, resulting in a decrease in the JHRR-driven reporter activity leading to reduction of Kr-h1 expression. These data show that Hsp83 facilitates the JH-induced nuclear import of Met that induces Kr-h1 expression through the JHRR. PMID:25122763

  15. The Drosophila FTZ-F1 Nuclear Receptor Mediates Juvenile Hormone Activation of E75A Gene Expression through an Intracellular Pathway*

    PubMed Central

    Dubrovsky, Edward B.; Dubrovskaya, Veronica A.; Bernardo, Travis; Otte, Valerie; DiFilippo, Robert; Bryan, Heather

    2011-01-01

    Juvenile hormone (JH) regulates a wide variety of biological activities in holometabolous insects, ranging from vitellogenesis and caste determination in adults to the timing of metamorphosis in larvae. The mechanism of JH signaling in such a diverse array of processes remains either unknown or contentious. We previously found that the nuclear receptor gene E75A is activated in S2 cells as a primary response to JH. Here, by expressing an intracellular form of JH esterase, we demonstrate that JH must enter the cell in order to activate E75A. To find intracellular receptors involved in the JH response, we performed an RNAi screen against nuclear receptor genes expressed in this cell line and identified the orphan receptor FTZ-F1. Removal of FTZ-F1 prevents JH activation of E75A, whereas overexpression enhances activation, implicating FTZ-F1 as a critical component of the JH response. FTZ-F1 is bound in vivo to multiple enhancers upstream of E75A, suggesting that it participates in direct JH-mediated gene activation. To better define the role of FTZ-F1 in JH signaling, we investigated interactions with candidate JH receptors and found that the bHLH-PAS proteins MET and GCE both interact with FTZ-F1 and can activate transcription through the FTZ-F1 response element. Removal of endogenous GCE, but not MET, prevents JH activation of E75A. We propose that FTZ-F1 functions as a competence factor by loading JH signaling components to the promoter, thus facilitating the direct regulation of E75A gene expression by JH. PMID:21832074

  16. Comparative ovarian microarray analysis of juvenile hormone-responsive genes in water flea Daphnia magna: potential targets for toxicity.

    PubMed

    Toyota, Kenji; Williams, Timothy D; Sato, Tomomi; Tatarazako, Norihisa; Iguchi, Taisen

    2017-03-01

    The freshwater zooplankton Daphnia magna has been extensively employed in chemical toxicity tests such as OECD Test Guidelines 202 and 211. Previously, it has been demonstrated that the treatment of juvenile hormones (JHs) or their analogues to female daphnids can induce male offspring production. Based on this finding, a rapid screening method for detection of chemicals with JH-activity was recently developed using adult D. magna. This screening system determines whether a chemical has JH-activity by investigating the male offspring inducibility. Although this is an efficient high-throughput short-term screening system, much remains to be discovered about JH-responsive pathways in the ovary, and whether different JH-activators act via the same mechanism. JH-responsive genes in the ovary including developing oocytes are still largely undescribed. Here, we conducted comparative microarray analyses using ovaries from Daphnia magna treated with fenoxycarb (Fx; artificial JH agonist) or methyl farnesoate (MF; a putative innate JH in daphnids) to elucidate responses to JH agonists in the ovary, including developing oocytes, at a JH-sensitive period for male sex determination. We demonstrate that induction of hemoglobin genes is a well-conserved response to JH even in the ovary, and a potential adverse effect of JH agonist is suppression of vitellogenin gene expression, that might cause reduction of offspring number. This is the first report demonstrating different transcriptomics profiles from MF and an artificial JH agonist in D. magna ovary, improving understanding the tissue-specific mode-of-action of JH. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  17. Parasitoid-host endocrine relations: self-reliance or co-optation?

    PubMed

    Cole, T J; Beckage, N E; Tan, F F; Srinivasan, A; Ramaswamy, S B

    2002-12-01

    High titers of juvenile hormone (JH) maintain developmental arrest in Manduca sexta larvae parasitized by Cotesia congregata. Parasitized hosts exhibit up to 9.5 times greater amounts of total hemolymph JH (from 0.6+/-0.09 to 2.51+/-0.43ng/ml) compared to non-parasitized controls. Elevated titers are observed throughout the fifth instar, even beyond egression of the parasitoids on day 5. GC-MS analysis revealed that in hemolymph of unparasitized control larvae, JH I is the major homolog and levels of JH III are negligible; in parasitized individuals the amounts of JH I, II, and III rise, and JH III predominates. Neck ligation ensured separation of M. sexta's corpora allata from the posterior section, which contained most of the parasitoids in the infected insects. When the posterior region was sampled, JHs were not detected in the non-parasitzed larvae, but in those parasitized, JH III was found (1.98+/-0.29ng/ml, 24 h post-ligation). JH III was the only homolog produced and secreted by the parasitoid in in vitro culture. This is the first report stating that a parasitoid secretes JH III and may contribute, at least in part, to the circulating titer in the host hemocoel, concurrently promoting host production of JH I and II.

  18. Drosophila Kruppel homolog 1 represses lipolysis through interactions with dFOXO

    USDA-ARS?s Scientific Manuscript database

    Juvenile hormone (JH) is a key endocrine signal involved in insect molting and metamorphosis. Recent studies suggest that JH is involved in not only development programming, but also in metabolic control. However, how JH modulates metabolism remains largely unknown. It has been shown that JH induces...

  19. Methyl Farnesoate Plays a Dual Role in Regulating Drosophila Metamorphosis

    PubMed Central

    Wen, Di; Rivera-Perez, Crisalejandra; Abdou, Mohamed; Jia, Qiangqiang; He, Qianyu; Liu, Xi; Zyaan, Ola; Xu, Jingjing; Bendena, William G.; Tobe, Stephen S.; Noriega, Fernando G.; Palli, Subba R.; Wang, Jian; Li, Sheng

    2015-01-01

    Corpus allatum (CA) ablation results in juvenile hormone (JH) deficiency and pupal lethality in Drosophila. The fly CA produces and releases three sesquiterpenoid hormones: JH III bisepoxide (JHB3), JH III, and methyl farnesoate (MF). In the whole body extracts, MF is the most abundant sesquiterpenoid, followed by JHB3 and JH III. Knockout of JH acid methyl transferase (jhamt) did not result in lethality; it decreased biosynthesis of JHB3, but MF biosynthesis was not affected. RNAi-mediated reduction of 3-hydroxy-3-methylglutaryl CoA reductase (hmgcr) expression in the CA decreased biosynthesis and titers of the three sesquiterpenoids, resulting in partial lethality. Reducing hmgcr expression in the CA of the jhamt mutant further decreased MF titer to a very low level, and caused complete lethality. JH III, JHB3, and MF function through Met and Gce, the two JH receptors, and induce expression of Kr-h1, a JH primary-response gene. As well, a portion of MF is converted to JHB3 in the hemolymph or peripheral tissues. Topical application of JHB3, JH III, or MF precluded lethality in JH-deficient animals, but not in the Met gce double mutant. Taken together, these experiments show that MF is produced by the larval CA and released into the hemolymph, from where it exerts its anti-metamorphic effects indirectly after conversion to JHB3, as well as acting as a hormone itself through the two JH receptors, Met and Gce. PMID:25774983

  20. Wolbachia-induced paternal defect in Drosophila is likely by interaction with the juvenile hormone pathway.

    PubMed

    Liu, Chen; Wang, Jia-Lin; Zheng, Ya; Xiong, En-Juan; Li, Jing-Jing; Yuan, Lin-Ling; Yu, Xiao-Qiang; Wang, Yu-Feng

    2014-06-01

    Wolbachia are endosymbionts that infect many insect species. They can manipulate the host's reproduction to increase their own maternal transmission. Cytoplasmic incompatibility (CI) is one such manipulation, which is expressed as embryonic lethality when Wolbachia-infected males mate with uninfected females. However, matings between males and females carrying the same Wolbachia strain result in viable progeny. The molecular mechanisms of CI are currently not clear. We have previously reported that the gene Juvenile hormone-inducible protein 26 (JhI-26) exhibited the highest upregulation in the 3rd instar larval testes of Drosophila melanogaster when infected by Wolbachia. This is reminiscent of an interaction between Wolbachia and juvenile hormone (JH) pathway in flies. Considering that Jhamt gene encodes JH acid methyltransferase, a key regulatory enzyme of JH biosynthesis, and that methoprene-tolerant (Met) has been regarded as the best JH receptor candidate, we first compared the expression of Jhamt and Met between Wolbachia-infected and uninfected fly testes to investigate whether Wolbachia infection influence the JH signaling pathway. We found that the expressions of Jhamt and Met were significantly increased in the presence of Wolbachia, suggesting an interaction of Wolbachia with the JH signaling pathway. Then, we found that overexpression of JhI-26 in Wolbachia-free transgenic male flies caused paternal-effect lethality that mimics the defects associated with CI. JhI-26 overexpressing males resulted in significantly decrease in hatch rate. Surprisingly, Wolbachia-infected females could rescue the egg hatch. In addition, we showed that overexpression of JhI-26 caused upregulation of the male accessory gland protein (Acp) gene CG10433, but not vice versa. This result suggests that JhI-26 may function at the upstream of CG10433. Likewise, overexpression of CG10433 also resulted in paternal-effect lethality. Both JhI-26 and CG10433 overexpressing males

  1. Neuropeptides affecting the transfer of juvenile hormones from males to females during mating in Spodoptera frugiperda.

    PubMed

    Hassanien, Intisar T E; Grötzner, Manuela; Meyering-Vos, Martina; Hoffmann, Klaus H

    2014-07-01

    In the polyandric moth, Spodopterafrugiperda, juvenile hormone (JH) is transferred from the male accessory reproductive glands (AG) to the female bursa copulatrix (BC) during copulation (see Hassanien et al., 2014). Here we used the RNA interference technique to study the role of allatoregulating neuropeptides in controlling the synthesis and transfer of JH during mating. Knockdown of S. frugiperda allatostatin C (Spofr-AS type C) in freshly emerged males leads to an accumulation of JH in the AG beyond that in the control and mating results in a higher transport of JH I and JH II into the female BC. Knockdown of S. frugiperda allatotropin 2 (Spofr-AT2) significantly reduces the amount of JH in the AG as well as its transfer into the female BC during copulation. Knockdown of S. frugiperda allatostatin A (Spofr-AS type A) and S. frugiperda allatotropin (Spofr-AT; Hassanien et al., 2014) only slightly affects the accumulation of JH in the AG and its transfer from the male to the female. We conclude that Spofr-AS type C and Spofr-AT2 act as true allatostatin and true allatotropin, respectively, on the synthesis of JH I and JH II in the male AG. Moreover, both peptides seem to control the synthesis of JH III in the corpora allata of adult males and its release into the hemolymph. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Genetic Evidence for Function of the bHLH-PAS Protein Gce/Met As a Juvenile Hormone Receptor

    PubMed Central

    Jindra, Marek; Uhlirova, Mirka; Charles, Jean-Philippe; Smykal, Vlastimil; Hill, Ronald J.

    2015-01-01

    Juvenile hormones (JHs) play a major role in controlling development and reproduction in insects and other arthropods. Synthetic JH-mimicking compounds such as methoprene are employed as potent insecticides against significant agricultural, household and disease vector pests. However, a receptor mediating effects of JH and its insecticidal mimics has long been the subject of controversy. The bHLH-PAS protein Methoprene-tolerant (Met), along with its Drosophila melanogaster paralog germ cell-expressed (Gce), has emerged as a prime JH receptor candidate, but critical evidence that this protein must bind JH to fulfill its role in normal insect development has been missing. Here, we show that Gce binds a native D. melanogaster JH, its precursor methyl farnesoate, and some synthetic JH mimics. Conditional on this ligand binding, Gce mediates JH-dependent gene expression and the hormone's vital role during development of the fly. Any one of three different single amino acid mutations in the ligand-binding pocket that prevent binding of JH to the protein block these functions. Only transgenic Gce capable of binding JH can restore sensitivity to JH mimics in D. melanogaster Met-null mutants and rescue viability in flies lacking both Gce and Met that would otherwise die at pupation. Similarly, the absence of Gce and Met can be compensated by expression of wild-type but not mutated transgenic D. melanogaster Met protein. This genetic evidence definitively establishes Gce/Met in a JH receptor role, thus resolving a long-standing question in arthropod biology. PMID:26161662

  3. Gonadotropic and Physiological Functions of Juvenile Hormone in Bumblebee (Bombus terrestris) Workers

    PubMed Central

    Shpigler, Hagai; Amsalem, Etya; Huang, Zachary Y.; Cohen, Mira; Siegel, Adam J.; Hefetz, Abraham; Bloch, Guy

    2014-01-01

    The evolution of advanced sociality in bees is associated with apparent modifications in juvenile hormone (JH) signaling. By contrast to most insects in which JH is a gonadotropin regulating female fertility, in the highly eusocial honey bee (Apis mellifera) JH has lost its gonadotrophic function in adult females, and instead regulates age-related division of labor among worker bees. In order to shed light on the evolution of JH signaling in bees we performed allatectomy and replacement therapies to manipulate JH levels in workers of the "primitively eusocial" bumblebee Bombus terrestris. Allatectomized worker bees showed remarkable reduction in ovarian development, egg laying, Vitellogenin and Krüppel homolog 1 fat body transcript levels, hemolymph Vitellogenin protein abundance, wax secretion, and egg-cell construction. These effects were reverted, at least partially, by treating allatectomized bees with JH-III, the natural JH of bees. Allatectomy also affected the amount of ester component in Dufour's gland secretion, which is thought to convey a social signal relating to worker fertility. These findings provide a strong support for the hypothesis that in contrast to honey bees, JH is a gonadotropin in bumblebees and lend credence to the hypothesis that the evolution of advanced eusociality in honey bees was associated with major modifications in JH signaling. PMID:24959888

  4. Juvenile Hormone Prevents 20-Hydroxyecdysone-induced Metamorphosis by Regulating the Phosphorylation of a Newly Identified Broad Protein*

    PubMed Central

    Cai, Mei-Juan; Liu, Wen; Pei, Xu-Yang; Li, Xiang-Ru; He, Hong-Juan; Wang, Jin-Xing; Zhao, Xiao-Fan

    2014-01-01

    The steroid hormone 20-hydroxyecdysone (20E) initiates insect molting and metamorphosis. By contrast, juvenile hormone (JH) prevents metamorphosis. However, the mechanism by which JH inhibits metamorphosis remains unclear. In this study, we propose that JH induces the phosphorylation of Broad isoform Z7 (BrZ7), a newly identified protein, to inhibit 20E-mediated metamorphosis in the lepidopteran insect Helicoverpa armigera. The knockdown of BrZ7 in larvae inhibited metamorphosis by repressing the expression of the 20E response gene. BrZ7 was weakly expressed and phosphorylated during larval growth but highly expressed and non-phosphorylated during metamorphosis. JH regulated the rapid phosphorylation of BrZ7 via a G-protein-coupled receptor-, phospholipase C-, and protein kinase C-triggered pathway. The phosphorylated BrZ7 bound to the 5′-regulatory region of calponin to regulate its expression in the JH pathway. Exogenous JH induced BrZ7 phosphorylation to prevent metamorphosis by suppressing 20E-related gene transcription. JH promoted non-phosphorylated calponin interacting with ultraspiracle protein to activate the JH pathway and antagonize the 20E pathway. This study reveals one of the possible mechanisms by which JH counteracts 20E-regulated metamorphosis by inducing the phosphorylation of BrZ7. PMID:25096576

  5. Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila.

    PubMed

    Liu, Ying; Sheng, Zhentao; Liu, Hanhan; Wen, Di; He, Qianyu; Wang, Sheng; Shao, Wei; Jiang, Rong-Jing; An, Shiheng; Sun, Yaning; Bendena, William G; Wang, Jian; Gilbert, Lawrence I; Wilson, Thomas G; Song, Qisheng; Li, Sheng

    2009-06-01

    Juvenile hormone (JH) regulates many developmental and physiological events in insects, but its molecular mechanism remains conjectural. Here we report that genetic ablation of the corpus allatum cells of the Drosophila ring gland (the JH source) resulted in JH deficiency, pupal lethality and precocious and enhanced programmed cell death (PCD) of the larval fat body. In the fat body of the JH-deficient animals, Dronc and Drice, two caspase genes that are crucial for PCD induced by the molting hormone 20-hydroxyecdysone (20E), were significantly upregulated. These results demonstrated that JH antagonizes 20E-induced PCD by restricting the mRNA levels of Dronc and Drice. The antagonizing effect of JH on 20E-induced PCD in the fat body was further confirmed in the JH-deficient animals by 20E treatment and RNA interference of the 20E receptor EcR. Moreover, MET and GCE, the bHLH-PAS transcription factors involved in JH action, were shown to induce PCD by upregulating Dronc and Drice. In the Met- and gce-deficient animals, Dronc and Drice were downregulated, whereas in the Met-overexpression fat body, Dronc and Drice were significantly upregulated leading to precocious and enhanced PCD, and this upregulation could be suppressed by application of the JH agonist methoprene. For the first time, we demonstrate that JH counteracts MET and GCE to prevent caspase-dependent PCD in controlling fat body remodeling and larval-pupal metamorphosis in Drosophila.

  6. Stage-specific regulation of juvenile hormone biosynthesis by ecdysteroid in Bombyx mori.

    PubMed

    Kaneko, Yu; Kinjoh, Terunori; Kiuchi, Makoto; Hiruma, Kiyoshi

    2011-03-30

    In the penultimate (4th) instar larvae of Bombyx mori, juvenile hormone (JH) synthesis by corpora allata (CA) fluctuates. When diet containing 20-hydroxyecdysone (20E) was fed, JH synthetic activity of the CA was first stimulated as the ecdysteroid titer increased, then suppressed slightly by the higher molting concentration of ecdysteroids (>250 ng/ml). The overall JH biosynthetic activity was modulated by the expression of JH biosynthetic enzymes in the CA: primarily JH acid O-methyltransferase (JHAMT), isopentenyl diphosphate isomerase, and farnesyl diphosphate synthase 1. After the last (5th) larval ecdysis, the artificially increased high ecdysteroid level due to the 20E diet activated JH synthesis by the CA, which required intact nervous connections with the brain. A factor(s) from the 20E-activated brain controls mainly JHAMT and HMG Co-A reductase expression to stimulate the JH synthesis. In the normal last instar larvae, the ecdysteroid titer declines so that these activation mechanisms are absent; therefore the decline of the ecdysteroid titer after the final larval ecdysis is one of the factors which induces the cessation of the JH synthesis by CA. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  7. Juvenile hormone prevents 20-hydroxyecdysone-induced metamorphosis by regulating the phosphorylation of a newly identified broad protein.

    PubMed

    Cai, Mei-Juan; Liu, Wen; Pei, Xu-Yang; Li, Xiang-Ru; He, Hong-Juan; Wang, Jin-Xing; Zhao, Xiao-Fan

    2014-09-19

    The steroid hormone 20-hydroxyecdysone (20E) initiates insect molting and metamorphosis. By contrast, juvenile hormone (JH) prevents metamorphosis. However, the mechanism by which JH inhibits metamorphosis remains unclear. In this study, we propose that JH induces the phosphorylation of Broad isoform Z7 (BrZ7), a newly identified protein, to inhibit 20E-mediated metamorphosis in the lepidopteran insect Helicoverpa armigera. The knockdown of BrZ7 in larvae inhibited metamorphosis by repressing the expression of the 20E response gene. BrZ7 was weakly expressed and phosphorylated during larval growth but highly expressed and non-phosphorylated during metamorphosis. JH regulated the rapid phosphorylation of BrZ7 via a G-protein-coupled receptor-, phospholipase C-, and protein kinase C-triggered pathway. The phosphorylated BrZ7 bound to the 5'-regulatory region of calponin to regulate its expression in the JH pathway. Exogenous JH induced BrZ7 phosphorylation to prevent metamorphosis by suppressing 20E-related gene transcription. JH promoted non-phosphorylated calponin interacting with ultraspiracle protein to activate the JH pathway and antagonize the 20E pathway. This study reveals one of the possible mechanisms by which JH counteracts 20E-regulated metamorphosis by inducing the phosphorylation of BrZ7. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. A mosquito hemolymph odorant-binding protein family member specifically binds juvenile hormone

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kim, Il Hwan; Pham, Van; Jablonka, Willy

    Juvenile hormone (JH) is a key regulator of insect development and reproduction. In adult mosquitoes, it is essential for maturation of the ovary and normal male reproductive behavior, but how JH distribution and activity is regulated after secretion is unclear. Here, we report a new type of specific JH-binding protein, given the name mosquito juvenile hormone-binding protein (mJHBP), which circulates in the hemolymph of pupal and adult Aedes aegypti males and females. mJHBP is a member of the odorant-binding protein (OBP) family, and orthologs are present in the genomes of Aedes, Culex, and Anopheles mosquito species. Using isothermal titration calorimetry,more » we show that mJHBP specifically binds JH II and JH III but not eicosanoids or JH derivatives. mJHBP was crystallized in the presence of JH III and found to have a double OBP domain structure reminiscent of salivary “long” D7 proteins of mosquitoes. We observed that a single JH III molecule is contained in the N-terminal domain binding pocket that is closed in an apparent conformational change by a C-terminal domain-derived α-helix. The electron density for the ligand indicated a high occupancy of the natural 10R enantiomer of JH III. Of note, mJHBP is structurally unrelated to hemolymph JHBP from lepidopteran insects. A low level of expression of mJHBP in Ae. aegypti larvae suggests that it is primarily active during the adult stage where it could potentially influence the effects of JH on egg development, mating behavior, feeding, or other processes.« less

  9. Sexual dimorphism of sleep regulated by juvenile hormone signaling in Drosophila

    PubMed Central

    Zhang, Enyan; Du, Juan; Liu, Suning; Price, Jeffrey

    2018-01-01

    Sexually dimorphic phenotypes are a universal phenomenon in animals. In the model animal fruit fly Drosophila, males and females exhibit long- and short-sleep phenotypes, respectively. However, the mechanism is still a mystery. In this study, we showed that juvenile hormone (JH) is involved in regulation of sexually dimorphic sleep in Drosophila, in which gain of JH function enlarges differences of the dimorphic sleep phenotype with higher sleep in males and lower sleep in females, while loss of JH function blurs these differences and results in feminization of male sleep and masculinization of female sleep. Further studies indicate that germ cell-expressed (GCE), one of the JH receptors, mediates the response in the JH pathway because the sexually dimorphic sleep phenotypes cannot be rescued by JH hormone in a gce deletion mutant. The JH-GCE regulated sleep dimorphism is generated through the sex differentiation-related genes -fruitless (fru) and doublesex (dsx) in males and sex-lethal (sxl), transformer (tra) and doublesex (dsx) in females. These are the “switch” genes that separately control the sleep pattern in males and females. Moreover, analysis of sleep deprivation and circadian behaviors showed that the sexually dimorphic sleep induced by JH signals is a change of sleep drive and independent of the circadian clock. Furthermore, we found that JH seems to also play an unanticipated role in antagonism of an aging-induced sleep decrease in male flies. Taken together, these results indicate that the JH signal pathway is critical for maintenance of sexually dimorphic sleep by regulating sex-relevant genes. PMID:29617359

  10. A mosquito hemolymph odorant-binding protein family member specifically binds juvenile hormone.

    PubMed

    Kim, Il Hwan; Pham, Van; Jablonka, Willy; Goodman, Walter G; Ribeiro, José M C; Andersen, John F

    2017-09-15

    Juvenile hormone (JH) is a key regulator of insect development and reproduction. In adult mosquitoes, it is essential for maturation of the ovary and normal male reproductive behavior, but how JH distribution and activity is regulated after secretion is unclear. Here, we report a new type of specific JH-binding protein, given the name mosquito juvenile hormone-binding protein (mJHBP), which circulates in the hemolymph of pupal and adult Aedes aegypti males and females. mJHBP is a member of the odorant-binding protein (OBP) family, and orthologs are present in the genomes of Aedes , Culex , and Anopheles mosquito species. Using isothermal titration calorimetry, we show that mJHBP specifically binds JH II and JH III but not eicosanoids or JH derivatives. mJHBP was crystallized in the presence of JH III and found to have a double OBP domain structure reminiscent of salivary "long" D7 proteins of mosquitoes. We observed that a single JH III molecule is contained in the N-terminal domain binding pocket that is closed in an apparent conformational change by a C-terminal domain-derived α-helix. The electron density for the ligand indicated a high occupancy of the natural 10 R enantiomer of JH III. Of note, mJHBP is structurally unrelated to hemolymph JHBP from lepidopteran insects. A low level of expression of mJHBP in Ae. aegypti larvae suggests that it is primarily active during the adult stage where it could potentially influence the effects of JH on egg development, mating behavior, feeding, or other processes.

  11. TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis.

    PubMed

    Ishimaru, Yoshiyasu; Tomonari, Sayuri; Matsuoka, Yuji; Watanabe, Takahito; Miyawaki, Katsuyuki; Bando, Tetsuya; Tomioka, Kenji; Ohuchi, Hideyo; Noji, Sumihare; Mito, Taro

    2016-05-17

    Although butterflies undergo a dramatic morphological transformation from larva to adult via a pupal stage (holometamorphosis), crickets undergo a metamorphosis from nymph to adult without formation of a pupa (hemimetamorphosis). Despite these differences, both processes are regulated by common mechanisms that involve 20-hydroxyecdysone (20E) and juvenile hormone (JH). JH regulates many aspects of insect physiology, such as development, reproduction, diapause, and metamorphosis. Consequently, strict regulation of JH levels is crucial throughout an insect's life cycle. However, it remains unclear how JH synthesis is regulated. Here, we report that in the corpora allata of the cricket, Gryllus bimaculatus, Myoglianin (Gb'Myo), a homolog of Drosophila Myoglianin/vertebrate GDF8/11, is involved in the down-regulation of JH production by suppressing the expression of a gene encoding JH acid O-methyltransferase, Gb'jhamt In contrast, JH production is up-regulated by Decapentaplegic (Gb'Dpp) and Glass-bottom boat/60A (Gb'Gbb) signaling that occurs as part of the transcriptional activation of Gb'jhamt Gb'Myo defines the nature of each developmental transition by regulating JH titer and the interactions between JH and 20E. When Gb'myo expression is suppressed, the activation of Gb'jhamt expression and secretion of 20E induce molting, thereby leading to the next instar before the last nymphal instar. Conversely, high Gb'myo expression induces metamorphosis during the last nymphal instar through the cessation of JH synthesis. Gb'myo also regulates final insect size. Because Myo/GDF8/11 and Dpp/bone morphogenetic protein (BMP)2/4-Gbb/BMP5-8 are conserved in both invertebrates and vertebrates, the present findings provide common regulatory mechanisms for endocrine control of animal development.

  12. Juvenile hormone levels reflect social opportunities in the facultatively eusocial sweat bee Megalopta genalis (Hymenoptera: Halictidae).

    PubMed

    Smith, Adam R; Kapheim, Karen M; Pérez-Ortega, Betzi; Brent, Colin S; Wcislo, William T

    2013-01-01

    The evolution of eusociality is hypothesized to have involved de-coupling parental care from reproduction mediated by changes in endocrine regulation. While data for obligately eusocial insects are consistent with this hypothesis, we lack information from species representative of the transition from solitary reproduction to eusociality. Here we report the first evidence for a link between endocrine processes and social behavior in a facultatively eusocial bee, Megalopta genalis (Halictidae). Using females that varied in social, reproductive, and ecological context, we measured juvenile hormone (JH), a major regulator of colony caste dynamics in other eusocial species. JH was low at adult emergence, but elevated after 10 days in all nesting females. Females reared in cages with ad lib nutrition, however, did not elevate JH levels after 10 days. All reproductive females had significantly more JH than all age-matched non-reproductive females, suggesting a gonadotropic function. Among females in established nests, JH was higher in queens than workers and solitary reproductives, suggesting a role for JH in social dominance. A lack of significant differences in JH between solitary reproductives and non-reproductive workers suggests that JH content reflects more than reproductive status. Our data support the hypothesis that endocrine modifications are involved in the evolutionary decoupling of reproductive and somatic effort in social insects. These are the first measurements of JH in a solitary-nesting hymenopteran, and the first to compare eusocial and solitary nesting individuals of the same species. Published by Elsevier Inc.

  13. Molecular impact of juvenile hormone agonists on neonatal Daphnia magna.

    PubMed

    Toyota, Kenji; Kato, Yasuhiko; Miyakawa, Hitoshi; Yatsu, Ryohei; Mizutani, Takeshi; Ogino, Yukiko; Miyagawa, Shinichi; Watanabe, Hajime; Nishide, Hiroyo; Uchiyama, Ikuo; Tatarazako, Norihisa; Iguchi, Taisen

    2014-05-01

    Daphnia magna has been used extensively to evaluate organism- and population-level responses to pollutants in acute toxicity and reproductive toxicity tests. We have previously reported that exposure to juvenile hormone (JH) agonists results in a reduction of reproductive function and production of male offspring in a cyclic parthenogenesis, D. magna. Recent advances in molecular techniques have provided tools to understand better the responses to pollutants in aquatic organisms, including D. magna. DNA microarray was used to evaluate gene expression profiles of neonatal daphnids exposed to JH agonists: methoprene (125, 250 and 500 ppb), fenoxycarb (0.5, 1 and 2 ppb) and epofenonane (50, 100 and 200 ppb). Exposure to these JH analogs resulted in chemical-specific patterns of gene expression. The heat map analyses based on hierarchical clustering revealed a similar pattern between treatments with a high dose of methoprene and with epofenonane. In contrast, treatment with low to middle doses of methoprene resulted in similar profiles to fenoxycarb treatments. Hemoglobin and JH epoxide hydrolase genes were clustered as JH-responsive genes. These data suggest that fenoxycarb has high activity as a JH agonist, methoprene shows high toxicity and epofenonane works through a different mechanism compared with other JH analogs, agreeing with data of previously reported toxicity tests. In conclusion, D. magna DNA microarray is useful for the classification of JH analogs and identification of JH-responsive genes. Copyright © 2013 John Wiley & Sons, Ltd.

  14. Increased juvenile hormone levels after long-duration flight in the grasshopper, Melanoplus sanguinipes.

    PubMed

    Min, Kyung Jin; Jones, Nathan; Borst, David W; Rankin, Mary Ann

    2004-06-01

    Although, in many insects, migration imposes a cost in terms of timing or amount of reproduction, in the migratory grasshopper Melanoplus sanguinipes performance of long-duration flight to voluntary cessation or exhaustion accelerates the onset of first reproduction and enhances reproductive success over the entire lifetime of the insect. Since juvenile hormone (JH) is involved in the control of reproduction in most species, we examined JH titer after long flight using a chiral selective radioimmunoassay. JH levels increased on days 5 and 8 in animals flown to exhaustion on day 4 but not in 1-h or non-flier controls. No difference was seen in the diel pattern of JH titer, but hemolymph samples were taken between 5 and 7 h after lights on. Treatment of grasshoppers with JH-III mimicked the effect of long-duration flight in the induction of early reproduction. The increased JH titer induced by performance of long-duration flight is thus at least one component of flight-enhanced reproduction. To test the possibility that post-flight JH titer increases are caused by adipokinetic hormone (AKH) released during long flights, a series of injections of physiological doses of Lom-AKH I were given to unflown animals to simulate AKH release during long flight. This treatment had no effect on JH titers. Thus, although AKH is released during flight and controls lipid mobilization, it is not the factor responsible for increased JH titers after long-duration flight.

  15. Identification and action of juvenile hormone III from sexually mature alate females of the red imported fire ant, Solenopsis invicta.

    PubMed

    Burns, S N.; Teal, P E.A.; Vander Meer, R K.; Nation, J L.; Vogt, J T.

    2002-03-01

    Analysis of extracts of hemolymph obtained from sexually mature alate females of Solenopsis invicta from monogyne colonies resulted in identification of juvenile hormone III (JH III). The average amount of JH III was 0.32+/-0.04 pmol/&mgr;molof hemolymph. Topical application of 0.038 pmol of JH III was sufficient to stimulate alates to shed their wings in the presence of the queen. The time in which alates were induced to dealate decreased linearly with increasing concentrations of JH III from 0.038 to 3.8 pmol. However, higher JH III concentrations deviated from linearity and did not reach dealation times comparable with those that occur after mating flights. Thus, it appears that the mechanism of dealation that occurs when female alates are out of the influence of their queen is different from the one associated with mating flights. Application of 0.42 &mgr;mol of precocene II inhibited dealation of alates in queenless colonies. However, this inhibition was reversed after applying 38 pmol JH III to precocene-treated alates. The sizes of corpora allata (CA) from sexuals treated with JH III did not differ from those of controls. However, the sizes of CA were reduced in alates treated with precocene II. The results indicated that JH was important to dealation.

  16. Enantioselective separation of racemic juvenile hormone III by normal-phase high-performance liquid chromatography and preparation of [(2)H(3)]juvenile hormone III as an internal standard for liquid chromatography-mass spectrometry quantification.

    PubMed

    Ichikawa, Akio; Ono, Hiroshi; Furuta, Kenjiro; Shiotsuki, Takahiro; Shinoda, Tetsuro

    2007-08-17

    Juvenile hormone III (JH III) racemate was prepared from methyl (2E,6E)-farnesoate via epoxidation with 3-chloroperbenzoic acid (mCPBA). Enantioselective separation of JH III was conducted using normal-phase high-performance liquid chromatography (HPLC) on a chiral stationary phase. [(2)H(3)]Methyl (2E,6E)-farnesoate was also prepared from (2E,6E)-farnesoic acid and [(2)H(4)]methanol (methanol-d(4)) using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 4-dimethylaminopyridine (DMAP); the conjugated double bond underwent isomerization to some degree. Epoxidation of [(2)H(3)]methyl (2E,6E)-farnesoate with mCPBA gave a novel deuterium-substituted internal standard [(2)H(3)]JH III (JH III-d(3)). The standard curve was produced by linear regression using the peak area ratios of JH III and JH III-d(3) in liquid chromatography-mass spectrometry (LC-MS).

  17. TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis

    PubMed Central

    Ishimaru, Yoshiyasu; Tomonari, Sayuri; Matsuoka, Yuji; Watanabe, Takahito; Miyawaki, Katsuyuki; Bando, Tetsuya; Tomioka, Kenji; Ohuchi, Hideyo; Noji, Sumihare; Mito, Taro

    2016-01-01

    Although butterflies undergo a dramatic morphological transformation from larva to adult via a pupal stage (holometamorphosis), crickets undergo a metamorphosis from nymph to adult without formation of a pupa (hemimetamorphosis). Despite these differences, both processes are regulated by common mechanisms that involve 20-hydroxyecdysone (20E) and juvenile hormone (JH). JH regulates many aspects of insect physiology, such as development, reproduction, diapause, and metamorphosis. Consequently, strict regulation of JH levels is crucial throughout an insect’s life cycle. However, it remains unclear how JH synthesis is regulated. Here, we report that in the corpora allata of the cricket, Gryllus bimaculatus, Myoglianin (Gb’Myo), a homolog of Drosophila Myoglianin/vertebrate GDF8/11, is involved in the down-regulation of JH production by suppressing the expression of a gene encoding JH acid O-methyltransferase, Gb’jhamt. In contrast, JH production is up-regulated by Decapentaplegic (Gb’Dpp) and Glass-bottom boat/60A (Gb’Gbb) signaling that occurs as part of the transcriptional activation of Gb’jhamt. Gb’Myo defines the nature of each developmental transition by regulating JH titer and the interactions between JH and 20E. When Gb’myo expression is suppressed, the activation of Gb’jhamt expression and secretion of 20E induce molting, thereby leading to the next instar before the last nymphal instar. Conversely, high Gb’myo expression induces metamorphosis during the last nymphal instar through the cessation of JH synthesis. Gb’myo also regulates final insect size. Because Myo/GDF8/11 and Dpp/bone morphogenetic protein (BMP)2/4-Gbb/BMP5–8 are conserved in both invertebrates and vertebrates, the present findings provide common regulatory mechanisms for endocrine control of animal development. PMID:27140602

  18. Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum.

    PubMed

    Minakuchi, Chieka; Ishii, Fumika; Washidu, Yumiko; Ichikawa, Akio; Tanaka, Toshiharu; Miura, Ken; Shinoda, Tetsuro

    2015-09-01

    Juvenile hormone (JH) is synthesized and secreted by the corpora allata. In the final two steps of JH biosynthesis, farnesoic acid (FA) is converted to JH through methylation by JH acid O-methyltransferase (JHAMT) and epoxidation by the cytochrome P450 enzyme CYP15. In the present study, we identified a homolog of CYP15 from the red flour beetle Tribolium castaneum (TcCYP15A1), and analyzed its expression as well as its role in JH biosynthesis. Quantitative RT-PCR analysis showed that the level of TcCYP15A1 mRNA was high in the embryonic stage as well as in the middle of the final larval instar. In the embryonic stage, the transcript level of TcCYP15A1 started to increase 30h after egg laying (AEL), peaked 54-60h AEL, and was followed by an increase of TcJHAMT mRNA, suggesting that JH biosynthesis started at this time point. TcCYP15A1 mRNA was present, but not exclusively so in the larval corpora allata. The recombinant TcCYP15A1 protein epoxidized both FA and methyl farnesoate (MF) in highly stereo-specific manners. These results confirmed that TcCYP15A1 is involved in JH biosynthesis. The RNAi-mediated knockdown of TcCYP15A1 in the pre-final larval instar did not result in precocious metamorphosis to pupa, indicating that MF may exhibit JH-like activity in order to maintain the larval status. The double knockdown of TcJHAMT and TcCYP15A1 resulted in pupae and adults with shorter wings, suggesting that the precursors of JH, JH acid and MF, may be essential for wing expansion. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. Activities of natural methyl farnesoids on pupariation and metamorphosis of Drosophila melanogaster

    USDA-ARS?s Scientific Manuscript database

    Methyl farnesoate (MF) and juvenile hormone (JH III), which respectively bind to the receptors USP and MET, and bisepoxy JH III (bisJHIII) were assessed for several activities during Drosophila larval development, and during prepupal development to eclosed adults. Dietary MF and JH III were similar...

  20. Coupling between H+ transport and anaerobic glycolysis in turtle urinary bladder: effect of inhibitors of H+ ATPase

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Steinmetz, P.R.; Husted, R.F.; Mueller, A.

    1981-03-15

    The coupling between H+ transport (JH) and anaerobic glycolysis was examined in vitro in an anaerobic preparation of turtle urinary bladder. JH was measured as the short-circuit current after Na+ transport was abolished with ouabain and by pH stat titration. The media were gassed with N2 and 1% CO2 (PO2 less than 0.5 mm Hg) and contained 10 mM glucose. Under these conditions, JH was not inhibited by 3 mM serosal (S) cyanide or by 0.1 mM mucosal (M) dinitrophenol. Control anaerobic lactate production (Jlac) of 47 bladders was plotted as a function of simultaneously measured JH. The slope ofmore » Jlac on JH was 0.58« less

  1. The effect of juvenile hormone on Polistes wasp fertility varies with cooperative behavior.

    PubMed

    Tibbetts, Elizabeth A; Sheehan, Michael J

    2012-04-01

    Social insects provide good models for studying how and why the mechanisms that underlie reproduction vary, as there is dramatic reproductive plasticity within and between species. Here, we test how the effect of juvenile hormone (JH) on fertility covaries with cooperative behavior in workers and nest-founding queens in the primitively eusocial wasp Polistes metricus. P. metricus foundresses and workers appear morphologically similar and both are capable of reproduction, though there is variation in the extent of social cooperation and the probability of reproduction across castes. Do the endocrine mechanisms that mediate reproduction co-vary with cooperative behavior? We found dramatic differences in the effect of JH on fertility across castes. In non-cooperative nest-founding queens, all individuals responded to JH by increasing their fertility. However, in cooperative workers, the effect of JH on fertility varies with body weight; large workers increase their fertility in response to JH while small workers do not. The variation in JH response may be an adaptation to facilitate resource allocation based on the probability of independent reproduction. This work contrasts with previous studies in closely related Polistes dominulus paper wasps, in which both foundresses and workers form cooperative associations and both castes show similar, condition-dependent JH response. The variation in JH responsiveness within and between species suggests that endocrine responsiveness and the factors influencing caste differentiation are surprisingly evolutionarily labile. Copyright © 2012 Elsevier Inc. All rights reserved.

  2. Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1

    PubMed Central

    Makihira, Seicho; Shuto, Takahiro; Nikawa, Hiroki; Okamoto, Keishi; Mine, Yuichi; Takamoto, Yuko; Ohara, Masaru; Tsuji, Koichiro

    2010-01-01

    The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration. PMID:20480030

  3. Methyl farnesoate epoxidase (mfe) gene expression and juvenile hormone titers in the life cycle of a highly eusocial stingless bee, Melipona scutellaris.

    PubMed

    Cardoso-Júnior, Carlos Antônio Mendes; Silva, Renato Pereira; Borges, Naiara Araújo; de Carvalho, Washington João; Walter, S Leal; Simões, Zilá Luz Paulino; Bitondi, Marcia Maria Gentile; Ueira Vieira, Carlos; Bonetti, Ana Maria; Hartfelder, Klaus

    2017-08-01

    In social insects, juvenile hormone (JH) has acquired novel functions related to caste determination and division of labor among workers, and this is best evidenced in the honey bee. In contrast to honey bees, stingless bees are a much more diverse group of highly eusocial bees, and the genus Melipona has long called special attention due to a proposed genetic mechanism of caste determination. Here, we examined methyl farnesoate epoxidase (mfe) gene expression, encoding an enzyme relevant for the final step in JH biosynthesis, and measured the hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers. We confirmed that mfe is exclusively expressed in the corpora allata. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation was, however, seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers we found that JH titers are lower in foragers than in nurse bees, while mfe expression did not differ. Stingless bees are, thus, distinct from honey bee workers, suggesting that they have maintained the ancestral gonadotropic function for JH. Hence, the physiological circuitries underlying a highly eusocial life style may be variable, even within a monophyletic clade such as the corbiculate bees. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Depletion of juvenile hormone esterase extends larval growth in Bombyx mori.

    PubMed

    Zhang, Zhongjie; Liu, Xiaojing; Shiotsuki, Takahiro; Wang, Zhisheng; Xu, Xia; Huang, Yongping; Li, Muwang; Li, Kai; Tan, Anjiang

    2017-02-01

    Two major hormones, juvenile hormone (JH) and 20-hydroxyecdysone (20E), regulate insect growth and development according to their precisely coordinated titres, which are controlled by both biosynthesis and degradation pathways. Juvenile hormone esterase (JHE) is the primary JH-specific degradation enzyme that plays a key role in regulating JH titers, along with JH epoxide hydrolase (JHEH) and JH diol kinase (JHDK). In the current study, a loss-of-function analysis of JHE in the silkworm, Bombyx mori, was performed by targeted gene disruption using the transgenic CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/RNA-guided Cas9 nucleases) system. Depletion of B. mori JHE (BmJHE) resulted in the extension of larval stages, especially the penultimate and ultimate larval stages, without deleterious effects to silkworm physiology. The expression of JHEH and JHDK was upregulated in mutant animals, indicating the existence of complementary routes in the JH metabolism pathway in which inactivation of one enzyme will activate other enzymes. RNA-Seq analysis of mutant animals revealed that genes involved in protein processing in the endoplasmic reticulum and in amino acid metabolism were affected by BmJHE depletion. Depletion of JHE and subsequent delayed JH metabolism activated genes in the TOR pathway, which are ultimately responsible for extending larval growth. The transgenic Cas9 system used in the current study provides a promising approach for analysing the actions of JH, especially in nondrosophilid insects. Furthermore, prolonging larval stages produced larger larvae and cocoons, which is greatly beneficial to silk production. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Differential impacts of juvenile hormone, soldier head extract and alternate caste phenotypes on host and symbiont transcriptome composition in the gut of the termite Reticulitermes flavipes

    PubMed Central

    2013-01-01

    Background Termites are highly eusocial insects and show a division of labor whereby morphologically distinct individuals specialize in distinct tasks. In the lower termite Reticulitermes flavipes (Rhinotermitidae), non-reproducing individuals form the worker and soldier castes, which specialize in helping (e.g., brood care, cleaning, foraging) and defense behaviors, respectively. Workers are totipotent juveniles that can either undergo status quo molts or develop into soldiers or neotenic reproductives. This caste differentiation can be regulated by juvenile hormone (JH) and primer pheromones contained in soldier head extracts (SHE). Here we offered worker termites a cellulose diet treated with JH or SHE for 24-hr, or held them with live soldiers (LS) or live neotenic reproductives (LR). We then determined gene expression profiles of the host termite gut and protozoan symbionts concurrently using custom cDNA oligo-microarrays containing 10,990 individual ESTs. Results JH was the most influential treatment (501 total ESTs affected), followed by LS (24 ESTs), LR (12 ESTs) and SHE treatments (6 ESTs). The majority of JH up- and downregulated ESTs were of host and symbiont origin, respectively; in contrast, SHE, LR and LS treatments had more uniform impacts on host and symbiont gene expression. Repeat “follow-up” bioassays investigating combined JH + SHE impacts in relation to individual JH and SHE treatments on a subset of array-positive genes revealed (i) JH and SHE treatments had opposite impacts on gene expression and (ii) JH + SHE impacts on gene expression were generally intermediate between JH and SHE. Conclusions Our results show that JH impacts hundreds of termite and symbiont genes within 24-hr, strongly suggesting a role for the termite gut in JH-dependent caste determination. Additionally, differential impacts of SHE and LS treatments were observed that are in strong agreement with previous studies that specifically investigated soldier caste

  6. Differential impacts of juvenile hormone, soldier head extract and alternate caste phenotypes on host and symbiont transcriptome composition in the gut of the termite Reticulitermes flavipes.

    PubMed

    Sen, Ruchira; Raychoudhury, Rhitoban; Cai, Yunpeng; Sun, Yijun; Lietze, Verena-Ulrike; Boucias, Drion G; Scharf, Michael E

    2013-07-19

    Termites are highly eusocial insects and show a division of labor whereby morphologically distinct individuals specialize in distinct tasks. In the lower termite Reticulitermes flavipes (Rhinotermitidae), non-reproducing individuals form the worker and soldier castes, which specialize in helping (e.g., brood care, cleaning, foraging) and defense behaviors, respectively. Workers are totipotent juveniles that can either undergo status quo molts or develop into soldiers or neotenic reproductives. This caste differentiation can be regulated by juvenile hormone (JH) and primer pheromones contained in soldier head extracts (SHE). Here we offered worker termites a cellulose diet treated with JH or SHE for 24-hr, or held them with live soldiers (LS) or live neotenic reproductives (LR). We then determined gene expression profiles of the host termite gut and protozoan symbionts concurrently using custom cDNA oligo-microarrays containing 10,990 individual ESTs. JH was the most influential treatment (501 total ESTs affected), followed by LS (24 ESTs), LR (12 ESTs) and SHE treatments (6 ESTs). The majority of JH up- and downregulated ESTs were of host and symbiont origin, respectively; in contrast, SHE, LR and LS treatments had more uniform impacts on host and symbiont gene expression. Repeat "follow-up" bioassays investigating combined JH + SHE impacts in relation to individual JH and SHE treatments on a subset of array-positive genes revealed (i) JH and SHE treatments had opposite impacts on gene expression and (ii) JH + SHE impacts on gene expression were generally intermediate between JH and SHE. Our results show that JH impacts hundreds of termite and symbiont genes within 24-hr, strongly suggesting a role for the termite gut in JH-dependent caste determination. Additionally, differential impacts of SHE and LS treatments were observed that are in strong agreement with previous studies that specifically investigated soldier caste regulation. However, it is likely

  7. John Henryism Active Coping, Acculturation, and Psychological Health in Korean Immigrants.

    PubMed

    Logan, Jeongok G; Barksdale, Debra J; James, Sherman A; Chien, Lung-Chang

    2017-03-01

    This study aimed to explore the levels of John Henryism (JH) active coping and its association with acculturation status and psychological health (specifically perceived stress, acculturative stress, anxiety, and depression) in Korean immigrants to the United States. In 102 Korean immigrants, JH active coping was measured by the JH Scale; acculturation by the Bidimensional Acculturation Scale; perceived stress by the Perceived Stress Scale; acculturative stress by the Social, Attitudinal, Familial, and Environmental Scale; anxiety by the State Anxiety Subscale of the Spielberger State-Trait Anxiety Inventory; and depression by the Center for Epidemiological Studies Depression Scale. The levels of JH active coping in this sample of Korean immigrants appear to be lower than the levels reported in other racial groups. Independent of demographic factors, JH active coping was a significant predictor of higher acculturation status and better psychological health as indicated by lower levels of perceived stress, acculturative stress, anxiety, and depressive symptoms.

  8. Developmental link between sex and nutrition; doublesex regulates sex-specific mandible growth via juvenile hormone signaling in stag beetles.

    PubMed

    Gotoh, Hiroki; Miyakawa, Hitoshi; Ishikawa, Asano; Ishikawa, Yuki; Sugime, Yasuhiro; Emlen, Douglas J; Lavine, Laura C; Miura, Toru

    2014-01-01

    Sexual dimorphisms in trait expression are widespread among animals and are especially pronounced in ornaments and weapons of sexual selection, which can attain exaggerated sizes. Expression of exaggerated traits is usually male-specific and nutrition sensitive. Consequently, the developmental mechanisms generating sexually dimorphic growth and nutrition-dependent phenotypic plasticity are each likely to regulate the expression of extreme structures. Yet we know little about how either of these mechanisms work, much less how they might interact with each other. We investigated the developmental mechanisms of sex-specific mandible growth in the stag beetle Cyclommatus metallifer, focusing on doublesex gene function and its interaction with juvenile hormone (JH) signaling. doublesex genes encode transcription factors that orchestrate male and female specific trait development, and JH acts as a mediator between nutrition and mandible growth. We found that the Cmdsx gene regulates sex differentiation in the stag beetle. Knockdown of Cmdsx by RNA-interference in both males and females produced intersex phenotypes, indicating a role for Cmdsx in sex-specific trait growth. By combining knockdown of Cmdsx with JH treatment, we showed that female-specific splice variants of Cmdsx contribute to the insensitivity of female mandibles to JH: knockdown of Cmdsx reversed this pattern, so that mandibles in knockdown females were stimulated to grow by JH treatment. In contrast, mandibles in knockdown males retained some sensitivity to JH, though mandibles in these individuals did not attain the full sizes of wild type males. We suggest that moderate JH sensitivity of mandibular cells may be the default developmental state for both sexes, with sex-specific Dsx protein decreasing sensitivity in females, and increasing it in males. This study is the first to demonstrate a causal link between the sex determination and JH signaling pathways, which clearly interact to determine the

  9. Characteristics of H+ current transients induced by adverse H+ gradient pulses in toad bladder.

    PubMed

    Nero, A C; Schwartz, J H; Furtado, M R

    1987-10-01

    Acidification in the toad bladder occurs as a result of electrogenic H+ secretion (JH). When a pH gradient is applied in a stepwise fashion in the absence of exogenous CO2, JH decreases linearly with the mucosal (M) solution pH and is null when pHm is approximately 4.5. When pHm is returned to initial values (7.4) in a stepwise fashion, JH increases linearly with pHm. However, on this return, higher values of JH are initially obtained. To investigate this hysteresis, hemibladders mounted in chambers were used to measure the change in the H+ current before and after acid pulses were applied to the mucosal solution. In the absence of exogenous CO2, the application of graded acid pulses to mucosa for 1, 2, 4, and 8 min resulted in a graded decrease in JH. The restoration of pHm to 7.4 was followed by an immediate transient overshoot of reversed short-circuit current (Irsc), which was related to the time of exposure and the magnitude of the acid pulse. The longer the acid pulse or the larger the pulse, the greater the Irsc overshoot. The addition of protonophores, dinitrophenol, or salicylate, into the mucosal solution enhanced this overshoot. Similar Irsc overshoots could be obtained with the application of pulses of adverse electrical gradients. Introduction of exogenous CO2 into the system (3%) completely inhibited the overshoot in JH after an acid pulse. In conclusion, when pHm is decreased JH is reduced and the cell pH presumably decreases because of continued exit of alkali at the serosal side of the cell and entry of H+ from the mucosal solution. The decrease in cell pH then triggers the pump to produce a sharp overshoot in JH when pHm returns to 7.4.

  10. Developmental Link between Sex and Nutrition; doublesex Regulates Sex-Specific Mandible Growth via Juvenile Hormone Signaling in Stag Beetles

    PubMed Central

    Gotoh, Hiroki; Miyakawa, Hitoshi; Ishikawa, Asano; Ishikawa, Yuki; Sugime, Yasuhiro; Emlen, Douglas J.; Lavine, Laura C.; Miura, Toru

    2014-01-01

    Sexual dimorphisms in trait expression are widespread among animals and are especially pronounced in ornaments and weapons of sexual selection, which can attain exaggerated sizes. Expression of exaggerated traits is usually male-specific and nutrition sensitive. Consequently, the developmental mechanisms generating sexually dimorphic growth and nutrition-dependent phenotypic plasticity are each likely to regulate the expression of extreme structures. Yet we know little about how either of these mechanisms work, much less how they might interact with each other. We investigated the developmental mechanisms of sex-specific mandible growth in the stag beetle Cyclommatus metallifer, focusing on doublesex gene function and its interaction with juvenile hormone (JH) signaling. doublesex genes encode transcription factors that orchestrate male and female specific trait development, and JH acts as a mediator between nutrition and mandible growth. We found that the Cmdsx gene regulates sex differentiation in the stag beetle. Knockdown of Cmdsx by RNA-interference in both males and females produced intersex phenotypes, indicating a role for Cmdsx in sex-specific trait growth. By combining knockdown of Cmdsx with JH treatment, we showed that female-specific splice variants of Cmdsx contribute to the insensitivity of female mandibles to JH: knockdown of Cmdsx reversed this pattern, so that mandibles in knockdown females were stimulated to grow by JH treatment. In contrast, mandibles in knockdown males retained some sensitivity to JH, though mandibles in these individuals did not attain the full sizes of wild type males. We suggest that moderate JH sensitivity of mandibular cells may be the default developmental state for both sexes, with sex-specific Dsx protein decreasing sensitivity in females, and increasing it in males. This study is the first to demonstrate a causal link between the sex determination and JH signaling pathways, which clearly interact to determine the

  11. Endocrine mediated phenotypic plasticity: condition-dependent effects of juvenile hormone on dominance and fertility of wasp queens.

    PubMed

    Tibbetts, Elizabeth A; Izzo, Amanda S

    2009-11-01

    There has been increasing interest in the mechanisms that mediate behavioral and physiological plasticity across individuals with similar genotypes. Some of the most dramatic plasticity is found within and between social insect castes. For example, Polistes wasp queens can nest alone, dominate a group of cooperative queens, or act as worker-like subordinates who rarely reproduce. Previous work suggests that condition-dependent endocrine responses may play a role in plasticity between castes in the hymenoptera. Here, we test whether condition-dependent endocrine responses influence plasticity within castes in the wasp Polistes dominulus. We experimentally manipulate juvenile hormone (JH) titers in nest-founding queens and assess whether JH mediates variation in behavior and physiology. JH generally increased dominance and fertility of queens, but JH's effects were not uniform across individuals. JH had a stronger effect on the dominance and fertility of large individuals and individuals with facial patterns advertising high quality than on the dominance and fertility of small individuals and those advertising low quality. These results demonstrate that JH has condition-dependent effects. As such, they clarify how JH can mediate different behaviors in well nourished queens and poorly nourished workers. Many Polistes queens nest cooperatively with other queens, so condition-dependent hormonal responses provide a mechanism for queens to adaptively allocate energy based on their probability of successfully becoming the dominant queen. Research on the endocrine basis of plasticity often focuses on variation in endocrine titers alone. However, differential endocrine responses are likely to be a widespread mechanism mediating behavioral and physiological plasticity.

  12. Methoprene influences reproduction and flight capacity in adults of the rice leaf roller, Cnaphalocrocis medinalis (Guenểe) (Lepidoptera: Pyralidae).

    PubMed

    Sun, Bei-Bei; Jiang, Xing-Fu; Zhang, Lei; Stanley, David Warren; Luo, Li-Zhi; Long, Wei

    2013-01-01

    Juvenile hormone (JH) influences many aspects of insect biology, including oogenesis-flight syndrome tradeoffs between migration and reproduction. Drawing on studies of many migratory insects, we posed the hypothesis that JH influences migratory capacity and oogenesis in the rice leaf roller, Cnaphalocrocis medinalis. We treated adults moths (days 1, 2 and 3 postemergence) with the JH analog (JHA), methoprene, and then recorded the influences of JHA treatments on reproduction. JHA treatment on day 1 postemergence, but not on the other days, shortened the preoviposition period, although JHA did not influence total fecundity, oviposition period, or longevity. We infer day 1 postemergence is the JH-sensitive stage to influence reproduction. Therefore, we treated moths on day 1 postemergence with JHA and recorded flight capacity, flight muscle mass, and triacylglycerol (TAG) accumulation. JHA treatments did not influence flight speed, but led to reductions in flight durations and flight distances. At day 3 posttreatment (PT), JHA-treated females flew shorter times and less distance than the controls; JHA-treated males, however, only flew shorter times than the controls. JHA treatments led to reductions in flight muscle mass in females at days 2-3 PT and reductions in TAG content in females at day 3 PT, but, these parameters were not influenced by JHA in males. These findings strongly support our hypothesis, from which we infer that JH is a major driver in C. medinalis oogenesis-flight syndrome tradeoffs. Our data also reveal a JH-sensitive stage in adulthood during which JH influences the oocyte-flight syndrome in C. medinalis. © 2012 Wiley Periodicals, Inc.

  13. Ectomorphic somatotype and joint hypermobility are linked in panic and agoraphobic patients: a case-control study.

    PubMed

    Pailhez, Guillem; Rosado, Silvia; Baeza-Velasco, Carolina; Bulbena, Antonio

    2014-06-01

    To test whether there is an association between somatotype measures, joint hypermobility (JH), and panic and/or agoraphobia (PA). Sociodemographic characteristics, somatotype, and JH status were assessed in 60 patients (30 men and 30 women) with PA and 60 non-clinical controls, matched by age and gender. Patients and controls categorized by gender did not differ in terms of age, educational degree, marital status, or labour situation. There were significant differences between mean somatotype groups both in men and women. Men and women somatotype patients were significantly less endomorphic and more ectomorphic than controls. Hypermobility was significantly more frequent in both male and female patients. In the entire sample, 38.3% of patients and 13.3% of controls were categorized as ectomorphic (χ(2) = 8.5, p = 0.004). After adjusting for age and sex, ectomorphic somatotype was independently related to JH status [OR = 3.25, 95% CI 1.35-7.8, p = 0.008]. Since PA may be associated with JH, it is suggested that the relationship found between panic and ectomorphic somatotype might be mediated through JH.

  14. Molecular mechanism underlying juvenile hormone-mediated repression of precocious larval-adult metamorphosis.

    PubMed

    Kayukawa, Takumi; Jouraku, Akiya; Ito, Yuka; Shinoda, Tetsuro

    2017-01-31

    Juvenile hormone (JH) represses precocious metamorphosis of larval to pupal and adult transitions in holometabolous insects. The early JH-inducible gene Krüppel homolog 1 (Kr-h1) plays a key role in the repression of metamorphosis as a mediator of JH action. Previous studies demonstrated that Kr-h1 inhibits precocious larval-pupal transition in immature larva via direct transcriptional repression of the pupal specifier Broad-Complex (BR-C). JH was recently reported to repress the adult specifier gene Ecdysone-induced protein 93F (E93); however, its mechanism of action remains unclear. Here, we found that JH suppressed ecdysone-inducible E93 expression in the epidermis of the silkworm Bombyx mori and in a B. mori cell line. Reporter assays in the cell line revealed that the JH-dependent suppression was mediated by Kr-h1. Genome-wide ChIP-seq analysis identified a consensus Kr-h1 binding site (KBS, 14 bp) located in the E93 promoter region, and EMSA confirmed that Kr-h1 directly binds to the KBS. Moreover, we identified a C-terminal conserved domain in Kr-h1 essential for the transcriptional repression of E93 Based on these results, we propose a mechanism in which JH-inducible Kr-h1 directly binds to the KBS site upstream of the E93 locus to repress its transcription in a cell-autonomous manner, thereby preventing larva from bypassing the pupal stage and progressing to precocious adult development. These findings help to elucidate the molecular mechanisms regulating the metamorphic genetic network, including the functional significance of Kr-h1, BR-C, and E93 in holometabolous insect metamorphosis.

  15. Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations.

    PubMed

    Martínez-Rincón, Raúl O; Rivera-Pérez, Crisalejandra; Diambra, Luis; Noriega, Fernando G

    2017-01-01

    Juvenile hormone (JH) regulates development and reproductive maturation in insects. The corpora allata (CA) from female adult mosquitoes synthesize fluctuating levels of JH, which have been linked to the ovarian development and are influenced by nutritional signals. The rate of JH biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, which is the outcome of a complex interplay of changes in precursor pools and enzyme levels. A comprehensive study of the changes in enzymatic activities and precursor pool sizes have been previously reported for the mosquito Aedes aegypti JH biosynthesis pathway. In the present studies, we used two different quantitative approaches to describe and predict how changes in the individual metabolic reactions in the pathway affect JH synthesis. First, we constructed generalized additive models (GAMs) that described the association between changes in specific metabolite concentrations with changes in enzymatic activities and substrate concentrations. Changes in substrate concentrations explained 50% or more of the model deviances in 7 of the 13 metabolic steps analyzed. Addition of information on enzymatic activities almost always improved the fitness of GAMs built solely based on substrate concentrations. GAMs were validated using experimental data that were not included when the model was built. In addition, a system of ordinary differential equations (ODE) was developed to describe the instantaneous changes in metabolites as a function of the levels of enzymatic catalytic activities. The results demonstrated the ability of the models to predict changes in the flux of metabolites in the JH pathway, and can be used in the future to design and validate experimental manipulations of JH synthesis.

  16. The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm, Bombyx mori.

    PubMed

    Zeng, Baosheng; Huang, Yuping; Xu, Jun; Shiotsuki, Takahiro; Bai, Hua; Palli, Subba Reddy; Huang, Yongping; Tan, Anjiang

    2017-07-14

    Forkhead box O (FOXO) functions as the terminal transcription factor of the insulin signaling pathway and regulates multiple physiological processes in many organisms, including lifespan in insects. However, how FOXO interacts with hormone signaling to modulate insect growth and development is largely unknown. Here, using the transgene-based CRISPR/Cas9 system, we generated and characterized mutants of the silkworm Bombyx mori FOXO ( BmFOXO ) to elucidate its physiological functions during development of this lepidopteran insect. The BmFOXO mutant (FOXO-M) exhibited growth delays from the first larval stage and showed precocious metamorphosis, pupating at the end of the fourth instar (trimolter) rather than at the end of the fifth instar as in the wild-type (WT) animals. However, different from previous reports on precocious metamorphosis caused by juvenile hormone (JH) deficiency in silkworm mutants, the total developmental time of the larval period in the FOXO-M was comparable with that of the WT. Exogenous application of 20-hydroxyecdysone (20E) or of the JH analog rescued the trimolter phenotype. RNA-seq and gene expression analyses indicated that genes involved in JH degradation but not in JH biosynthesis were up-regulated in the FOXO-M compared with the WT animals. Moreover, we identified several FOXO-binding sites in the promoter of genes coding for JH-degradation enzymes. These results suggest that FOXO regulates JH degradation rather than its biosynthesis, which further modulates hormone homeostasis to control growth and development in B. mori In conclusion, we have uncovered a pivotal role for FOXO in regulating JH signaling to control insect development. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Deciding to have knee or hip replacement

    MedlinePlus

    ... joint replacement surgery References Harkess JW, Crockarell JR. Arthroplasty of the hip. In: Azar FM, Beaty JH, ... Philadelphia, PA: Elsevier; 2017:chap 3. Mihalko WM. Arthroplasty of the knee. In: Azar FM, Beaty JH, ...

  18. Leukemia

    MedlinePlus

    ... JH, Kastan MB, Tepper JE, eds. Abeloff's Clinical Oncology . 5th ed. Philadelphia, PA: Elsevier Saunders; 2014:chap ... JH, Kastan MB, Tepper JE, eds. Abeloff's Clinical Oncology . 5th ed. Philadelphia, PA: Elsevier Saunders; 2014:chap ...

  19. The POU Factor Ventral Veins Lacking/Drifter Directs the Timing of Metamorphosis through Ecdysteroid and Juvenile Hormone Signaling

    PubMed Central

    Chaieb, Leila; Koyama, Takashi; Sarwar, Prioty; Mirth, Christen K.; Smith, Wendy A.; Suzuki, Yuichiro

    2014-01-01

    Although endocrine changes are known to modulate the timing of major developmental transitions, the genetic mechanisms underlying these changes remain poorly understood. In insects, two developmental hormones, juvenile hormone (JH) and ecdysteroids, are coordinated with each other to induce developmental changes associated with metamorphosis. However, the regulation underlying the coordination of JH and ecdysteroid synthesis remains elusive. Here, we examined the function of a homolog of the vertebrate POU domain protein, Ventral veins lacking (Vvl)/Drifter, in regulating both of these hormonal pathways in the red flour beetle, Tribolium castaneum (Tenebrionidae). RNA interference-mediated silencing of vvl expression led to both precocious metamorphosis and inhibition of molting in the larva. Ectopic application of a JH analog on vvl knockdown larvae delayed the onset of metamorphosis and led to a prolonged larval stage, indicating that Vvl acts upstream of JH signaling. Accordingly, vvl knockdown also reduced the expression of a JH biosynthesis gene, JH acid methyltransferase 3 (jhamt3). In addition, ecdysone titer and the expression of the ecdysone response gene, hormone receptor 3 (HR3), were reduced in vvl knockdown larvae. The expression of the ecdysone biosynthesis gene phantom (phm) and spook (spo) were reduced in vvl knockdown larvae in the anterior and posterior halves, respectively, indicating that Vvl might influence ecdysone biosynthesis in both the prothoracic gland and additional endocrine sources. Injection of 20-hydroxyecdysone (20E) into vvl knockdown larvae could restore the expression of HR3 although molting was never restored. These findings suggest that Vvl coordinates both JH and ecdysteroid biosynthesis as well as molting behavior to influence molting and the timing of metamorphosis. Thus, in both vertebrates and insects, POU factors modulate the production of major neuroendocrine regulators during sexual maturation. PMID:24945490

  20. Molecular Determinants of Juvenile Hormone Action as Revealed by 3D QSAR Analysis in Drosophila

    PubMed Central

    Beňo, Milan; Farkaš, Robert

    2009-01-01

    Background Postembryonic development, including metamorphosis, of many animals is under control of hormones. In Drosophila and other insects these developmental transitions are regulated by the coordinate action of two principal hormones, the steroid ecdysone and the sesquiterpenoid juvenile hormone (JH). While the mode of ecdysone action is relatively well understood, the molecular mode of JH action remains elusive. Methodology/Principal Findings To gain more insights into the molecular mechanism of JH action, we have tested the biological activity of 86 structurally diverse JH agonists in Drosophila melanogaster. The results were evaluated using 3D QSAR analyses involving CoMFA and CoMSIA procedures. Using this approach we have generated both computer-aided and species-specific pharmacophore fingerprints of JH and its agonists, which revealed that the most active compounds must possess an electronegative atom (oxygen or nitrogen) at both ends of the molecule. When either of these electronegative atoms are replaced by carbon or the distance between them is shorter than 11.5 Å or longer than 13.5 Å, their biological activity is dramatically decreased. The presence of an electron-deficient moiety in the middle of the JH agonist is also essential for high activity. Conclusions/Significance The information from 3D QSAR provides guidelines and mechanistic scope for identification of steric and electrostatic properties as well as donor and acceptor hydrogen-bonding that are important features of the ligand-binding cavity of a JH target protein. In order to refine the pharmacophore analysis and evaluate the outcomes of the CoMFA and CoMSIA study we used pseudoreceptor modeling software PrGen to generate a putative binding site surrogate that is composed of eight amino acid residues corresponding to the defined molecular interactions. PMID:19547707

  1. Knockdown of the juvenile hormone receptor gene inhibits soldier-specific morphogenesis in the damp-wood termite Zootermopsis nevadensis (Isoptera: Archotermopsidae).

    PubMed

    Masuoka, Yudai; Yaguchi, Hajime; Suzuki, Ryutaro; Maekawa, Kiyoto

    2015-09-01

    The Methoprene-tolerant (Met) protein has been established as a juvenile hormone (JH) receptor. Knockdown of the Met gene caused precocious metamorphosis and suppression of ovarian development. However, the function of Met in caste development of social insects is unclear. In termites, JH acts as a central factor for caste development, especially for soldier differentiation, which involves two molts from workers via a presoldier stage. Increased JH titer in workers is needed for the presoldier molt, and the high JH titer is maintained throughout the presoldier period. Although presoldiers have the fundamental morphological features of soldiers, the nature of the cuticle is completely different from that of soldiers. We expected that JH signals via Met are involved in soldier-specific morphogenesis of the head and mandibles during soldier differentiation, especially in the presoldier period, in natural conditions. To test this hypothesis, we focused on soldier differentiation in an incipient colony of the damp-wood termite Zootermopsis nevadensis. Met homolog (ZnMet) expression in heads increased just after the presoldier molt. This high expression was reduced by ZnMet double stranded (dsRNA) injection before the presoldier molt. Although this treatment did not cause any morphological changes in presoldiers, it caused strong effects on soldiers, their mandibles being significantly shorter and head capsules smaller than those of control soldiers. Injection of ZnMet dsRNA throughout the presoldier stage did not affect the formation of soldier morphology, including cuticle formation. These results suggested that the rapid increase in ZnMet expression and subsequent activation of JH signaling just after the presoldier molt are needed for the formation of soldier-specific weapons. Therefore, besides its established role in insect metamorphosis, the JH receptor signaling also underlies soldier development in termites. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle

    PubMed Central

    Daimon, Takaaki; Uchibori, Miwa; Nakao, Hajime; Sezutsu, Hideki; Shinoda, Tetsuro

    2015-01-01

    Insect juvenile hormones (JHs) prevent precocious metamorphosis and allow larvae to undergo multiple rounds of status quo molts. However, the roles of JHs during the embryonic and very early larval stages have not been fully understood. We generated and characterized knockout silkworms (Bombyx mori) with null mutations in JH biosynthesis or JH receptor genes using genome-editing tools. We found that embryonic growth and morphogenesis are largely independent of JHs in Bombyx and that, even in the absence of JHs or JH signaling, pupal characters are not formed in first- or second-instar larvae, and precocious metamorphosis is induced after the second instar at the earliest. We also show by mosaic analysis that a pupal specifier gene broad, which is dramatically up-regulated in the late stage of the last larval instar, is essential for pupal commitment in the epidermis. Importantly, the mRNA expression level of broad, which is thought to be repressed by JHs, remained at very low basal levels during the early larval instars of JH-deficient or JH signaling-deficient knockouts. Therefore, our study suggests that the long-accepted paradigm that JHs maintain the juvenile status throughout larval life should be revised because the larval status can be maintained by a JH-independent mechanism in very early larval instars. We propose that the lack of competence for metamorphosis during the early larval stages may result from the absence of an unidentified broad-inducing factor, i.e., a competence factor. PMID:26195792

  3. Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle.

    PubMed

    Daimon, Takaaki; Uchibori, Miwa; Nakao, Hajime; Sezutsu, Hideki; Shinoda, Tetsuro

    2015-08-04

    Insect juvenile hormones (JHs) prevent precocious metamorphosis and allow larvae to undergo multiple rounds of status quo molts. However, the roles of JHs during the embryonic and very early larval stages have not been fully understood. We generated and characterized knockout silkworms (Bombyx mori) with null mutations in JH biosynthesis or JH receptor genes using genome-editing tools. We found that embryonic growth and morphogenesis are largely independent of JHs in Bombyx and that, even in the absence of JHs or JH signaling, pupal characters are not formed in first- or second-instar larvae, and precocious metamorphosis is induced after the second instar at the earliest. We also show by mosaic analysis that a pupal specifier gene broad, which is dramatically up-regulated in the late stage of the last larval instar, is essential for pupal commitment in the epidermis. Importantly, the mRNA expression level of broad, which is thought to be repressed by JHs, remained at very low basal levels during the early larval instars of JH-deficient or JH signaling-deficient knockouts. Therefore, our study suggests that the long-accepted paradigm that JHs maintain the juvenile status throughout larval life should be revised because the larval status can be maintained by a JH-independent mechanism in very early larval instars. We propose that the lack of competence for metamorphosis during the early larval stages may result from the absence of an unidentified broad-inducing factor, i.e., a competence factor.

  4. No Detectable Trade-Offs Among Immune Function, Fecundity, and Survival via a Juvenile Hormone Analog in the House Cricket.

    PubMed

    Nava-Sánchez, A; Munguía-Steyer, R; Córdoba-Aguilar, A

    2014-08-01

    Hormones are key regulators of resource allocation among functions and thus play an important role in resource-based trade-offs. The juvenile hormone (JH) is an insect hormone that mediates resource allocation between immunity and life history components. Here, we have tested whether this is the case using the house cricket. We investigated whether increased levels of JH (using methoprene, a JH analog) enable an enhanced survival and fecundity (via egg number) at the cost of reduced hemocyte number (a trait that is associated with immune response in insects) in the house cricket, Acheta domesticus L. We had three groups of adult crickets of both sexes: experimental (methoprene and acetone), positive control (methoprene), and negative control (no manipulation). Prior to and after experimental treatments, we counted the number of hemocytes (for the case of both sexes) and recorded the number of eggs laid and survival of females after the manipulation. There was no difference in hemocyte number, egg number, and survival. These results do not support a JH-mediated trade-off among immune ability, survival, and fecundity. We provide arguments to explain the lack of JH-mediated trade-offs in the house cricket.

  5. Juvenile hormone-binding proteins of Melanoplus bivittatus identified by EFDA photoaffinity labeling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Winder, B.S.

    1988-01-01

    Proteins that bind juvenile hormone in the hemolymph and fat body of the grasshopper, Melanoplus bivittatus were identified by photoaffinity labeling with radiolabeled epoxyfarnesyl diazoacetate ({sup 3}H-EFDA), and were characterized by electrophoretic analysis. A protocol was developed which allowed detection of {sup 3}H-EFDA that was covalently linked to proteins upon exposure to ultraviolet light at 254 nm. Quantification of protein-linked {sup 3}H-EFDA by liquid scintillation spectrometry took advantage of the differential solubility of unlinked {sup 3}H-EFDA in toluene alone, and of the protein-linked {sup 3}H-EFDA in toluene plus the detergent, Triton X-100. Competition between EFDA and juvenile hormone (JH) formore » binding to JH-specific binding sites was measured by hydroxyapatite protein binding assays in the presence of radiolabeled JH or EFDA and competing non-radiolabeled hormone. The protein-linked EFDA was detected on fluorograms of SDS or nondenaturing polyacrylamide gels (PAGE), and by liquid scintillation spectrometry of membranes to which the proteins had been electrophoretically transferred. Proteins which specifically bound JH were identified by photolabeling proteins in the presence and absence of nonlabeled JH-III.« less

  6. TRANSGENERATIONAL EFFECTS OF A JUVENILE HORMONE MIMIC ON THE ESTUARINE MYSID, MYSIDOPSIS BAHIA (CRUSTACEA: MYSIDACEA)

    EPA Science Inventory

    Fenoxycarb is a juvenile hormone (JH) mimic used to control insect pests by interfering with reproductive and developmental processes mediated by JH. Crustaceans are ideal organisms to monitor environmental effects of these endocrine disruptors, since they are dominant aquatic ar...

  7. Genetics Home Reference: complement component 2 deficiency

    MedlinePlus

    ... deficiency Sources for This Page Jönsson G, Sjöholm AG, Truedsson L, Bengtsson AA, Braconier JH, Sturfelt G. ... L, Sturfelt G, Oxelius VA, Braconier JH, Sjöholm AG. Hereditary C2 deficiency in Sweden: frequent occurrence of ...

  8. Methoprene influences reproduction and flight capacity in adults of the rice leaf roller, Cnaphalocrocis medinalis (Guenee) (Lepidoptera: Pyralidae)

    USDA-ARS?s Scientific Manuscript database

    Juvenile hormone (JH) influences many aspects of insect biology, including oogenesis-flight syndrome tradeoffs between migration and reproduction. Drawing on studies of many migratory insects, we posed the hypothesis that JH influences migratory capacity and oogenesis in the rice leaf roller, Cnapha...

  9. ISOLATION OF JUVENILE HORMONES ESTERASE AND ITS PARTIAL CDNA CLONE FROM THE BEETLE, TENEBRIO MOLITOR. (R825433)

    EPA Science Inventory

    Juvenile hormone esterase (JHE) plays an essential role in insect development. It is partially responsible for the clearance of juvenile hormone (JH) which regulates various aspects of insect development and reproduction. Because of its role in regulating JH titer, this enzyme...

  10. Differential gene expression in response to juvenile hormone analog treatment in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Archotermopsidae).

    PubMed

    Cornette, Richard; Hayashi, Yoshinobu; Koshikawa, Shigeyuki; Miura, Toru

    2013-04-01

    Termite societies are characterized by a highly organized division of labor among conspicuous castes, groups of individuals with various morphological specializations. Termite caste differentiation is under control of juvenile hormone (JH), but the molecular mechanism underlying the response to JH and early events triggering caste differentiation are still poorly understood. In order to profile candidate gene expression during early soldier caste differentiation of the damp-wood termite, Hodotermopsis sjostedti, we treated pseudergates (workers) with a juvenile hormone analog (JHA) to induce soldier caste differentiation. We then used Suppressive Subtractive Hybridization to create two cDNA libraries enriched for transcripts that were either up- or downregulated at 24h after treatment. Finally, we used quantitative PCR to confirm temporal expression patterns. Hexamerins represent a large proportion of the genes upregulated following JHA treatment and have an expression pattern that shows roughly an inverse correlation to intrinsic JH titers. This data is consistent with the role of a JH "sink", which was demonstrated for hexamerins in another termite, Reticulitermes flavipes. A putative nuclear protein was also upregulated a few hours after JHA treatment, which suggests a role in the early response to JH and subsequent regulation of transcriptional events associated with soldier caste differentiation. Some digestive enzymes, such as endogenous beta-endoglucanase and chymotrypsin, as well as a protein associated to digestion were identified among genes downregulated after JHA treatment. This suggests that JH may directly influence the pseudergate-specific digestive system. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. All polymer asymmetric Mach-Zehnder interferometer waveguide sensor by imprinting bonding and laser polishing

    NASA Astrophysics Data System (ADS)

    Liu, Yu; Sun, Yue; Yi, Yun-Ji; Tian, Liang; Cao, Yue; Chen, Chang-Ming; Sun, Xiao-Qiang; Zhang, Da-Ming

    2017-12-01

    Not Available Project supported by the National Natural Science Foundation of China (Grant Nos. 61605057, 61475061, and 61575076), the Science and Technology Development Plan of Jilin Province, China (Grant No. 20140519006JH), and the Excellent Youth Foundation of Jilin Province, China (Grant No. 20170520158JH).

  12. Precocene-I inhibits juvenile hormone biosynthesis, ovarian activation, aggression and alters sterility signal production in bumble bee (Bombus terrestris) workers

    USDA-ARS?s Scientific Manuscript database

    Juvenile hormone (JH) is an important regulator of development and physiology in insects. While in many insect species, including bumble bees, JH function as gonadotropin in adults, in some highly eusocial insects its role has shifted to regulate social behavior including division of labor, dominanc...

  13. Self, memory, and imagining the future in a case of psychogenic amnesia.

    PubMed

    Rathbone, Clare J; Ellis, Judi A; Baker, Ian; Butler, Chris R

    2015-01-01

    We report a case of psychogenic amnesia and examine the relationships between autobiographical memory impairment, the self, and ability to imagine the future. Case study JH, a 60-year-old male, experienced a 6-year period of pervasive psychogenic amnesia covering all life events from childhood to the age of 53. JH was tested during his amnesic period and again following hypnotherapy and the recovery of his memories. JH's amnesia corresponded with deficits in self-knowledge and imagining the future. Results are discussed with reference to models of self and memory and processes involving remembering and imagining.

  14. TOR Pathway-Mediated Juvenile Hormone Synthesis Regulates Nutrient-Dependent Female Reproduction in Nilaparvata lugens (Stål)

    PubMed Central

    Lu, Kai; Chen, Xia; Liu, Wen-Ting; Zhou, Qiang

    2016-01-01

    The “target of rapamycin” (TOR) nutritional signaling pathway and juvenile hormone (JH) regulation of vitellogenesis has been known for a long time. However, the interplay between these two pathways regulating vitellogenin (Vg) expression remains obscure. Here, we first demonstrated the key role of amino acids (AAs) in activation of Vg synthesis and egg development in Nilaparvata lugens using chemically defined artificial diets. AAs induced the expression of TOR and S6K (S6 kinase), whereas RNAi-mediated silencing of these two TOR pathway genes and rapamycin application strongly inhibited the AAs-induced Vg synthesis. Furthermore, knockdown of Rheb (Ras homologue enriched in brain), TOR, S6K and application of rapamycin resulted in a dramatic reduction in the mRNA levels of jmtN (juvenile hormone acid methyltransferase, JHAMT). Application of JH III on the RNAi (Rheb and TOR) and rapamycin-treated females partially rescued the Vg expression. Conversely, knockdown of either jmtN or met (methoprene-tolerant, JH receptor) and application of JH III had no effects on mRNA levels of Rheb, TOR and S6K and phosphorylation of S6K. In summary, our results demonstrate that the TOR pathway induces JH biosynthesis that in turn regulates AAs-mediated Vg synthesis in N. lugens. PMID:27043527

  15. TOR Pathway-Mediated Juvenile Hormone Synthesis Regulates Nutrient-Dependent Female Reproduction in Nilaparvata lugens (Stål).

    PubMed

    Lu, Kai; Chen, Xia; Liu, Wen-Ting; Zhou, Qiang

    2016-03-28

    The "target of rapamycin" (TOR) nutritional signaling pathway and juvenile hormone (JH) regulation of vitellogenesis has been known for a long time. However, the interplay between these two pathways regulating vitellogenin (Vg) expression remains obscure. Here, we first demonstrated the key role of amino acids (AAs) in activation of Vg synthesis and egg development in Nilaparvata lugens using chemically defined artificial diets. AAs induced the expression of TOR and S6K (S6 kinase), whereas RNAi-mediated silencing of these two TOR pathway genes and rapamycin application strongly inhibited the AAs-induced Vg synthesis. Furthermore, knockdown of Rheb (Ras homologue enriched in brain), TOR, S6K and application of rapamycin resulted in a dramatic reduction in the mRNA levels of jmtN (juvenile hormone acid methyltransferase, JHAMT). Application of JH III on the RNAi (Rheb and TOR) and rapamycin-treated females partially rescued the Vg expression. Conversely, knockdown of either jmtN or met (methoprene-tolerant, JH receptor) and application of JH III had no effects on mRNA levels of Rheb, TOR and S6K and phosphorylation of S6K. In summary, our results demonstrate that the TOR pathway induces JH biosynthesis that in turn regulates AAs-mediated Vg synthesis in N. lugens.

  16. Hormonal Interference with Pheromone Systems in Parasitic Acarines, Especially Ixodid Ticks.

    DTIC Science & Technology

    1984-05-01

    neuro- secretory morphology of the chicken mite Dermanyssus gallinae (Mesostigmata:Dermanyssidae). J. Morphol. (in press). *12. Oliver, J.H., Jr., J.M...13. Oliver, J.H., Jr., J.M. Pound and G. Severino. Evidence of a juvenile hormone-like compound in reproduction of Dermanyssus gallinae (Acari

  17. After chemotherapy - discharge

    MedlinePlus

    ... References Doroshow JH. Approach to the patient with cancer. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 179. Freifeld AG, Kaul DR. Infection in the patient with cancer. In: Niederhuber JE, Armitage JO, Doroshow JH, Kastan ...

  18. Organizational Assessment of the Depot Level Analysis Center for the Lockheed S-3 Viking Aircraft.

    DTIC Science & Technology

    1987-09-01

    Review, October 1979. 13. Gibson, J.L., Ivancevich , J.M., and Donnelly, J.H., Jr., Organizations, 4th ed., Business Publications, Inc., 1982. 14. Dickson...and Process, West Publishing Co., 1973. Gibson. J.L., Ivancevich , J.M., and Donnelly, J.H., Jr., Organizazions, 4th ed., Business Publications, nc

  19. Juvenile Hormone Regulation of Drosophila Epac - A Guanine Nucleotide Exchange Factor for Rap1 Small GTPase

    USDA-ARS?s Scientific Manuscript database

    Previously, we utilized a microchip array encompassing probes for 14,010 genes of Drosophila melanogaster to analyze the effect of (10R) juvenile hormone III (JH) on genome-wide gene expression in Drosophila S2 cells. Treatment with JH yielded a collection of 32 gene transcripts that demonstrated a ...

  20. Juvenile hormone and insulin suppress lipolysis between periods of lactation during tsetse fly pregnancy

    PubMed Central

    Michalkova, Veronika; Mireji, Paul; Attardo, Geoffrey M.; Moulton, John K.; Wilson, Thomas G.; Aksoy, Serap

    2014-01-01

    Tsetse flies are viviparous insects that nurture a single intrauterine progeny per gonotrophic cycle. The developing larva is nourished by the lipid-rich, milk-like secretions from a modified female accessory gland (milk gland). An essential feature of the lactation process involves lipid mobilization for incorporation into the milk. In this study, we examined roles for juvenile hormone (JH) and insulin/IGF-like (IIS) signaling pathways during tsetse pregnancy. In particular, we examined the roles for these pathways in regulating lipid homeostasis during transitions between non-lactating (dry) and lactating periods. The dry period occurs over the course of oogenesis and embryogenesis, while the lactation period spans intrauterine larvigenesis. Genes involved in the JH and IIS pathways were upregulated during dry periods, correlating with lipid accumulation between bouts of lactation. RNAi suppression of Forkhead Box Sub Group O (FOXO) expression impaired lipolysis during tsetse lactation and reduced fecundity. Similar reduction of the JH receptor Methoprene tolerant (Met), but not its paralog germ cell expressed (gce), reduced lipid accumulation during dry periods, indicating functional divergence between Met and gce during tsetse reproduction. Reduced lipid levels following Met knockdown led to impaired fecundity due to inadequate fat reserves at the initiation of milk production. Both the application of the JH analog (JHA) methoprene and injection of insulin into lactating females increased stored lipids by suppressing lipolysis and reduced transcripts of lactation-specific genes, leading to elevated rates of larval abortion. To our knowledge, this study is the first to address the molecular physiology of JH and IIS in a viviparous insect, and specifically to provide a role for JH signaling through Met in the regulation of lipid metabolism. PMID:23499946

  1. Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

    PubMed Central

    Bilen, Julide; Atallah, Jade; Azanchi, Reza; Levine, Joel D.; Riddiford, Lynn M.

    2013-01-01

    Juvenile hormone (JH) coordinates timing of female reproductive maturation in most insects. In Drosophila melanogaster, JH plays roles in both mating and egg maturation. However, very little is known about the molecular pathways associated with mating. Our behavioral analysis of females genetically lacking the corpora allata, the glands that produce JH, showed that they were courted less by males and mated later than control females. Application of the JH mimic, methoprene, to the allatectomized females just after eclosion rescued both the male courtship and the mating delay. Our studies of the null mutants of the JH receptors, Methoprene tolerant (Met) and germ cell-expressed (gce), showed that lack of Met in Met27 females delayed the onset of mating, whereas lack of Gce had little effect. The Met27 females were shown to be more attractive but less behaviorally receptive to copulation attempts. The behavioral but not the attractiveness phenotype was rescued by the Met genomic transgene. Analysis of the female cuticular hydrocarbon profiles showed that corpora allata ablation caused a delay in production of the major female-specific sex pheromones (the 7,11-C27 and -C29 dienes) and a change in the cuticular hydrocarbon blend. In the Met27 null mutant, by 48 h, the major C27 diene was greatly increased relative to wild type. In contrast, the gce2.5k null mutant females were courted similarly to control females despite changes in certain cuticular hydrocarbons. Our findings indicate that JH acts primarily via Met to modulate the timing of onset of female sex pheromone production and mating. PMID:24145432

  2. Cyp15F1: a novel cytochrome P450 gene linked to juvenile hormone-dependent caste differention in the termite Reticulitermes flavipes.

    PubMed

    Tarver, Matthew R; Coy, Monique R; Scharf, Michael E

    2012-07-01

    Termites are eusocial insects that jointly utilize juvenile hormone (JH), pheromones, and other semiochemicals to regulate caste differentiation and achieve caste homeostasis. Prior EST sequencing from the symbiont-free gut transcriptome of Reticulitermes flavipes unexpectedly revealed a number of unique cytochrome P450 (Cyp) transcripts, including fragments of a Cyp15 family gene (Cyp15F1) with homology to other insect Cyp15s that participate in JH biosynthesis. The present study investigated the role of Cyp15F1 in termite caste polyphenism and specifically tested the hypothesis that it plays a role in JH-dependent caste differentiation. After assembling the full-length Cyp15F1 cDNA sequence, we (i) determined its mRNA tissue expression profile, (ii) investigated mRNA expression changes in response to JH and the caste-regulatory primer pheromones γ-cadinene (CAD) and γ-cadinenal (ALD), and (iii) used RNA interference (RNAi) in combination with caste differentiation bioassays to investigate gene function at the phenotype level. Cyp15F1 has ubiquitous whole-body expression (including gut tissue); is rapidly and sustainably induced from 3 h to 48 h by JH, CAD, and ALD; and functions at least in part by facilitating JH-dependent soldier caste differentiation. These findings provide the second example of a termite caste regulatory gene identified through the use of RNAi, and significantly build upon our understanding of termite caste homeostatic mechanisms. These results also reinforce the concept of environmental caste determination in termites by revealing how primer pheromones, as socioenvironmental factors, can directly influence Cyp15 expression and caste differentiation. © 2012 Wiley Periodicals, Inc.

  3. Elliptic Solvers for Mediterranean Sea Ocean Modeling,

    DTIC Science & Technology

    1984-05-01

    KWSP =21*(112+2*21+6) C PARAMETER (NX=IH-2, NY=JHS-2, KQ=NY*((NX+7)/4+1)+(NY+3)/2+8) 9C DOUBLE PRECISION AX,AY,AC(KH),ACKL DIMENSION HD(IH,JH),HT(IH...JH),RS(IH,JH) C COMMON/BV/ W1(IH,JHS),W2(IH,JHS),W3(IH,JHS),W4(IH,JHS) DOUBLE PRECISION WQ DIMENSION MAP(IH,JHS),WQ(JHS,5),WC( KWSP ) EQUIVALENCE (W1(1...AND ALL OTHER MODES C ( TYPICALLY MXKC1 .GE. MXKC2 .GE .MXKC3 ), C MXKC3 = MAX SIZE OF CV FOR RESTART T.S. C ( TYPICALLY MXKC3 = MXKP*3 ). C KWSP

  4. Production, fertility, survival, and body measurements of Montbéliarde-sired crossbreds compared with pure Holsteins during their first 5 lactations.

    PubMed

    Hazel, A R; Heins, B J; Seykora, A J; Hansen, L B

    2014-01-01

    Two-breed crossbreds of Montbéliarde and Holstein (MO × HO) as well as 3-breed crossbreds of Montbéliarde and Jersey/Holstein (MO × JH) were compared with pure Holstein (HO) cows for production, somatic cell score (SCS), fertility, survival to subsequent calving, mortality, and body measurements during their first 5 lactations. Cows calved for the first time between 2005 and 2010 and were housed in either a confinement herd or a herd that had access to pasture for 165d of the year in the north central region of the United States. Body, hoof, and udder measurements of cows were also objectively measured. The MO × HO crossbred cows were not different from pure HO cows for fat-plus-protein production during any lactation. However, the MO × JH crossbred cows had 5% lower fat-plus-protein production compared with pure HO cows in the confinement herd. On the other hand, the MO × JH crossbred cows were not different for fat-plus-protein production in the third to fifth lactation compared with pure HO cows in the seasonal pasture herd. Across the 2 herds, the MO × HO and MO × JH crossbred cows had 21% higher first-service conception rate, 41 fewer days open, and 12% higher pregnancy rate compared with the pure HO cows. Furthermore, the MO × HO (5%) and MO × JH (12%) crossbred cows had lower mortality rates than the pure HO cows (18%). Because of superior fertility and lower mortality rates, the MO × HO and MO × JH crossbred cows, combined, had greater survival to second (+13%), third (+24%), fourth (+25%), and fifth (+17%) lactation compared with pure HO cows. For body measurements, MO × HO were similar to pure HO cows for hip height and heart girth, but MO × HO cows had more body condition and greater body weight (+39kg) across the first 5 lactations. The MO × JH cows had more body condition but 5cm shorter hip height and 28kg less body weight than pure HO cows across the first 5 lactations. Foot angle was steeper and hoof length was shorter for MO × HO

  5. Toward a Common Language for Measuring Patient Mobility in the Hospital: Reliability and Construct Validity of Interprofessional Mobility Measures.

    PubMed

    Hoyer, Erik H; Young, Daniel L; Klein, Lisa M; Kreif, Julie; Shumock, Kara; Hiser, Stephanie; Friedman, Michael; Lavezza, Annette; Jette, Alan; Chan, Kitty S; Needham, Dale M

    2018-02-01

    The lack of common language among interprofessional inpatient clinical teams is an important barrier to achieving inpatient mobilization. In The Johns Hopkins Hospital, the Activity Measure for Post-Acute Care (AM-PAC) Inpatient Mobility Short Form (IMSF), also called "6-Clicks," and the Johns Hopkins Highest Level of Mobility (JH-HLM) are part of routine clinical practice. The measurement characteristics of these tools when used by both nurses and physical therapists for interprofessional communication or assessment are unknown. The purposes of this study were to evaluate the reliability and minimal detectable change of AM-PAC IMSF and JH-HLM when completed by nurses and physical therapists and to evaluate the construct validity of both measures when used by nurses. A prospective evaluation of a convenience sample was used. The test-retest reliability and the interrater reliability of AM-PAC IMSF and JH-HLM for inpatients in the neuroscience department (n = 118) of an academic medical center were evaluated. Each participant was independently scored twice by a team of 2 nurses and 1 physical therapist; a total of 4 physical therapists and 8 nurses participated in reliability testing. In a separate inpatient study protocol (n = 69), construct validity was evaluated via an assessment of convergent validity with other measures of function (grip strength, Katz Activities of Daily Living Scale, 2-minute walk test, 5-times sit-to-stand test) used by 5 nurses. The test-retest reliability values (intraclass correlation coefficients) for physical therapists and nurses were 0.91 and 0.97, respectively, for AM-PAC IMSF and 0.94 and 0.95, respectively, for JH-HLM. The interrater reliability values (intraclass correlation coefficients) between physical therapists and nurses were 0.96 for AM-PAC IMSF and 0.99 for JH-HLM. Construct validity (Spearman correlations) ranged from 0.25 between JH-HLM and right-hand grip strength to 0.80 between AM-PAC IMSF and the Katz Activities of

  6. The Drosophila Juvenile Hormone Receptor Candidates Methoprene-tolerant (MET) and Germ Cell-expressed (GCE) Utilize a Conserved LIXXL Motif to Bind the FTZ-F1 Nuclear Receptor*

    PubMed Central

    Bernardo, Travis J.; Dubrovsky, Edward B.

    2012-01-01

    Juvenile hormone (JH) has been implicated in many developmental processes in holometabolous insects, but its mechanism of signaling remains controversial. We previously found that in Drosophila Schneider 2 cells, the nuclear receptor FTZ-F1 is required for activation of the E75A gene by JH. Here, we utilized insect two-hybrid assays to show that FTZ-F1 interacts with two JH receptor candidates, the bHLH-PAS paralogs MET and GCE, in a JH-dependent manner. These interactions are severely reduced when helix 12 of the FTZ-F1 activation function 2 (AF2) is removed, implicating AF2 as an interacting site. Through homology modeling, we found that MET and GCE possess a C-terminal α-helix featuring a conserved motif LIXXL that represents a novel nuclear receptor (NR) box. Docking simulations supported by two-hybrid experiments revealed that FTZ-F1·MET and FTZ-F1·GCE heterodimer formation involves a typical NR box-AF2 interaction but does not require the canonical charge clamp residues of FTZ-F1 and relies primarily on hydrophobic contacts, including a unique interaction with helix 4. Moreover, we identified paralog-specific features, including a secondary interaction site found only in MET. Our findings suggest that a novel NR box enables MET and GCE to interact JH-dependently with the AF2 of FTZ-F1. PMID:22249180

  7. Protein Kinase Pathways That Regulate Neuronal Survival and Death

    DTIC Science & Technology

    2004-08-01

    Prehn JH (1999) Mitochondrial depolarization Tagami M, Yamagata K, Nara Y, Fujino H, Kubota A, Numano F, is not required for neuronal apoptosis, J...Poppe M, Krieglstein J, Prehn C, Jones KR, Reichardt LF, Barbacid M (1991 b) The trkB tyrosine pro- JH (2002) p7 5 neurotrophin receptor is required

  8. The inverse microconglomerate test: Definition and application to the preservation of Paleoarchean to Hadean magnetizations in metasediments of the Jack Hills, Western Australia

    NASA Astrophysics Data System (ADS)

    Cottrell, Rory; Tarduno, John; Bono, Richard; Dare, Matthew

    2016-04-01

    We introduce a new paleomagnetic field test, the inverse microconglomerate test. In contrast with traditional conglomerate tests, which target specimens that might preserve primary magnetizations, the inverse microconglomerate test focuses on magnetic carriers having unblocking temperatures less than peak metamorphic temperatures. These mineral carriers are expected to carry a consistent direction of remagnetization. Hence, the inverse microconglomerate test evaluates whether coherent magnetizations are retained on a grain/mineral scale in a given sedimentary rock sample. By defining the remagnetization direction, it also serves as a benchmark for comparison of magnetizations from other grains/minerals having unblocking temperatures higher than peak metamorphic conditions (i.e., potential primary magnetizations). We apply this new test to sediments of the Jack Hills (JH), Yilgarn craton, Western Australia. For the JH sediments we focus on fuchsite, a secondary Cr-mica that contains relict Cr-Fe spinels capable of recording remanent magnetizations. We find that JH fuchsite grains retain consistent magnetic directions at unblocking temperatures between ˜270 and 340 oC, which defines a positive test. This direction does not reproduce a nominal 1078-1070 Ma remagnetization reported by Weiss et al. (EPSL, 2015) that we interpret as an artifact of inappropriate use of averaging and statistics. The thermochemical remanent magnetization recorded by the fuchsite was most likely imparted during peak JH metamorphic conditions at ˜2650 Ma. Our inverse microconglomerate test complements a positive microconglomerate test and large scale positive conglomerate test conducted on JH cobbles (Tarduno and Cottrell, EPSL, 2013), further supporting evidence that JH zircons record Paleoarchean to Hadean primary magnetizations at high (greater than 550 oC) unblocking temperatures (Tarduno et al., Science, 2015). More generally, the new inverse microconglomerate test may aid in

  9. Juvenile hormone and insulin suppress lipolysis between periods of lactation during tsetse fly pregnancy.

    PubMed

    Baumann, Aaron A; Benoit, Joshua B; Michalkova, Veronika; Mireji, Paul; Attardo, Geoffrey M; Moulton, John K; Wilson, Thomas G; Aksoy, Serap

    2013-06-15

    Tsetse flies are viviparous insects that nurture a single intrauterine progeny per gonotrophic cycle. The developing larva is nourished by the lipid-rich, milk-like secretions from a modified female accessory gland (milk gland). An essential feature of the lactation process involves lipid mobilization for incorporation into the milk. In this study, we examined roles for juvenile hormone (JH) and insulin/IGF-like (IIS) signaling pathways during tsetse pregnancy. In particular, we examined the roles for these pathways in regulating lipid homeostasis during transitions between non-lactating (dry) and lactating periods. The dry period occurs over the course of oogenesis and embryogenesis, while the lactation period spans intrauterine larvigenesis. Genes involved in the JH and IIS pathways were upregulated during dry periods, correlating with lipid accumulation between bouts of lactation. RNAi suppression of Forkhead Box Sub Group O (FOXO) expression impaired lipolysis during tsetse lactation and reduced fecundity. Similar reduction of the JH receptor Methoprene tolerant (Met), but not its paralog germ cell expressed (gce), reduced lipid accumulation during dry periods, indicating functional divergence between Met and gce during tsetse reproduction. Reduced lipid levels following Met knockdown led to impaired fecundity due to inadequate fat reserves at the initiation of milk production. Both the application of the JH analog (JHA) methoprene and injection of insulin into lactating females increased stored lipids by suppressing lipolysis and reduced transcripts of lactation-specific genes, leading to elevated rates of larval abortion. To our knowledge, this study is the first to address the molecular physiology of JH and IIS in a viviparous insect, and specifically to provide a role for JH signaling through Met in the regulation of lipid metabolism during insect lactation. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  10. Potentiation Effects of Half-Squats Performed in a Ballistic or Nonballistic Manner.

    PubMed

    Suchomel, Timothy J; Sato, Kimitake; DeWeese, Brad H; Ebben, William P; Stone, Michael H

    2016-06-01

    This study examined and compared the acute effects of ballistic and nonballistic concentric-only half-squats (COHSs) on squat jump performance. Fifteen resistance-trained men performed a squat jump 2 minutes after a control protocol or 2 COHSs at 90% of their 1 repetition maximum (1RM) COHS performed in a ballistic or nonballistic manner. Jump height (JH), peak power (PP), and allometrically scaled peak power (PPa) were compared using three 3 × 2 repeated-measures analyses of variance. Statistically significant condition × time interaction effects existed for JH (p = 0.037), PP (p = 0.041), and PPa (p = 0.031). Post hoc analysis revealed that the ballistic condition produced statistically greater JH (p = 0.017 and p = 0.036), PP (p = 0.031 and p = 0.026), and PPa (p = 0.024 and p = 0.023) than the control and nonballistic conditions, respectively. Small effect sizes for JH, PP, and PPa existed during the ballistic condition (d = 0.28-0.44), whereas trivial effect sizes existed during the control (d = 0.0-0.18) and nonballistic (d = 0.0-0.17) conditions. Large statistically significant relationships existed between the JH potentiation response and the subject's relative back squat 1RM (r = 0.520; p = 0.047) and relative COHS 1RM (r = 0.569; p = 0.027) during the ballistic condition. In addition, large statistically significant relationship existed between JH potentiation response and the subject's relative back squat strength (r = 0.633; p = 0.011), whereas the moderate relationship with the subject's relative COHS strength trended toward significance (r = 0.483; p = 0.068). Ballistic COHS produced superior potentiation effects compared with COHS performed in a nonballistic manner. Relative strength may contribute to the elicited potentiation response after ballistic and nonballistic COHS.

  11. Acute exercise alters skeletal muscle mitochondrial respiration and H2O2 emission in response to hyperinsulinemic-euglycemic clamp in middle-aged obese men

    PubMed Central

    Trewin, Adam J.; Levinger, Itamar; Parker, Lewan; Shaw, Christopher S.; Serpiello, Fabio R.; Anderson, Mitchell J.; McConell, Glenn K.; Hare, David L.

    2017-01-01

    Obesity, sedentary lifestyle and aging are associated with mitochondrial dysfunction and impaired insulin sensitivity. Acute exercise increases insulin sensitivity in skeletal muscle; however, whether mitochondria are involved in these processes remains unclear. The aim of this study was to investigate the effects of insulin stimulation at rest and after acute exercise on skeletal muscle mitochondrial respiratory function (JO2) and hydrogen peroxide emission (JH2O2), and the associations with insulin sensitivity in obese, sedentary men. Nine men (means ± SD: 57 ± 6 years; BMI 33 ± 5 kg.m2) underwent hyperinsulinemic-euglycemic clamps in two separate trials 1–3 weeks apart: one under resting conditions, and another 1 hour after high-intensity exercise (4x4 min cycling at 95% HRpeak). Muscle biopsies were obtained at baseline, and pre/post clamp to measure JO2 with high-resolution respirometry and JH2O2 via Amplex UltraRed from permeabilized fibers. Post-exercise, both JO2 and JH2O2 during ADP stimulated state-3/OXPHOS respiration were lower compared to baseline (P<0.05), but not after subsequent insulin stimulation. JH2O2 was lower post-exercise and after subsequent insulin stimulation compared to insulin stimulation in the rest trial during succinate supported state-4/leak respiration (P<0.05). In contrast, JH2O2 increased during complex-I supported leak respiration with insulin after exercise compared with resting conditions (P<0.05). Resting insulin sensitivity and JH2O2 during complex-I leak respiration were positively correlated (r = 0.77, P<0.05). We conclude that in obese, older and sedentary men, acute exercise modifies skeletal muscle mitochondrial respiration and H2O2 emission responses to hyperinsulinemia in a respiratory state-specific manner, which may have implications for metabolic diseases involving insulin resistance. PMID:29161316

  12. A novel cell penetrating peptide carrier for the delivery of nematocidal proteins drug

    NASA Astrophysics Data System (ADS)

    Kim, Jea Hyun

    Nematodes have recently become a primary source of harmful diseases to the environment that inflict harsh damages to pine trees and marine species. However, nematodes cannot be killed by normal pesticides or chemicals due to their thick outer protective layer mainly composed of collagen and cuticles. Thus, a novel approach to trigger intracellular delivery of chemicals through the layers of nematodes is required. In this study, the selection of the novel CPP was carefully progressed through protein database and serial digested fragmentation, internalization of each amino sequence was analyzed through flow cytometry and confocal microscope. As one of the most effective CPP material, JH 1.6 was compared with other major CPPs and its cellular toxicity was investigated. Furthermore, JH 1.6 was attached to various RNA, DNA, and proteins and internalization efficiency was evaluated for mammalian cells. To examine its effects on nematodes in vivo, JH 1.6 was conjugated with nematocidal protein - botulinum neurotoxin (BnT) and treated in C.elegans as a model animal. The results showed that JH 1.6 had high relative internalization rate and low cellular toxicity compared to other major CPP such as TAT and GV1001 peptides.

  13. Is John Henryism a resilience factor in women experiencing intimate partner violence?

    PubMed

    Kramer, Nicole M; Johnson, Nicole L; Johnson, Dawn M

    2015-01-01

    Research suggests that posttraumatic stress disorder (PTSD) and depression are two common mental health problems in intimate partner violence (IPV) survivors. Research has found that while Black women consistently report higher rates of victimization than White women, they also report less severe PTSD and depressive symptoms, suggesting that Black IPV survivors might be more resilient to PTSD and depression than are White survivors. We implemented a correlational study with 81 Black and 100 White female survivors of IPV to determine if John Henryism (JH; i.e., a predisposed active coping mechanism) contributes to the resilience observed in Black IPV survivors. Participants completed the John Henryism Active Coping Scale, Center for Epidemiological Studies Depression Scale, Davidson Trauma Scale, and the Abusive Behavior Inventory. Results demonstrated that White woman endorsed more severe depressive symptoms as compared to Black women. Severity of PTSD symptoms and JH was not significantly different between races. JH did not moderate the relationship between race and depression; however, for PTSD, JH was found to be protective of PTSD in White women, while demonstrating little impact on Black women. The implications of these findings are discussed in terms of the minority stress model.

  14. Juvenile hormone-dopamine systems for the promotion of flight activity in males of the large carpenter bee Xylocopa appendiculata.

    PubMed

    Sasaki, Ken; Nagao, Takashi

    2013-12-01

    The reproductive roles of dopamine and dopamine regulation systems are known in social hymenopterans, but the knowledge on the regulation systems in solitary species is still needed. To test the possibility that juvenile hormone (JH) and brain dopamine interact to trigger territorial flight behavior in males of a solitary bee species, the effects on biogenic amines of JH analog treatments and behavioral assays with dopamine injections in males of the large carpenter bee Xylocopa appendiculata were quantified. Brain dopamine levels were significantly higher in methoprene-treated males than in control males 4 days after treatment, but were not significantly different after 7 days. Brain octopamine and serotonin levels did not differ between methoprene-treated and control males at 4 and 7 days after treatment. Injection of dopamine caused significantly higher locomotor activities and a shorter duration for flight initiation in experimental versus control males. These results suggest that brain dopamine can be regulated by JH and enhances flight activities in males. The JH-dopamine system in males of this solitary bee species is similar to that of males of the highly eusocial honeybee Apis mellifera.

  15. Juvenile hormone-dopamine systems for the promotion of flight activity in males of the large carpenter bee Xylocopa appendiculata

    NASA Astrophysics Data System (ADS)

    Sasaki, Ken; Nagao, Takashi

    2013-12-01

    The reproductive roles of dopamine and dopamine regulation systems are known in social hymenopterans, but the knowledge on the regulation systems in solitary species is still needed. To test the possibility that juvenile hormone (JH) and brain dopamine interact to trigger territorial flight behavior in males of a solitary bee species, the effects on biogenic amines of JH analog treatments and behavioral assays with dopamine injections in males of the large carpenter bee Xylocopa appendiculata were quantified. Brain dopamine levels were significantly higher in methoprene-treated males than in control males 4 days after treatment, but were not significantly different after 7 days. Brain octopamine and serotonin levels did not differ between methoprene-treated and control males at 4 and 7 days after treatment. Injection of dopamine caused significantly higher locomotor activities and a shorter duration for flight initiation in experimental versus control males. These results suggest that brain dopamine can be regulated by JH and enhances flight activities in males. The JH-dopamine system in males of this solitary bee species is similar to that of males of the highly eusocial honeybee Apis mellifera.

  16. Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors

    PubMed Central

    Joseph, Joan; Fernández-Lloris, Raquel; Pezzat, Elías; Saubi, Narcís; Cardona, Pere-Joan; Mothe, Beatriz; Gatell, Josep Maria

    2010-01-01

    Mycobacterium bovis Bacillus Calmette-Guérin (BCG) as a live vector of recombinant bacterial vaccine is a promising system to be used. In this study, we evaluate the disrupted expression of heterologous HIV-1gp120 gene in BCG Pasteur host strain using replicative vectors pMV261 and pJH222. pJH222 carries a lysine complementing gene in BCG lysine auxotrophs. The HIV-1 gp120 gene expression was regulated by BCG hsp60 promoter (in plasmid pMV261) and Mycobacteria spp. α-antigen promoter (in plasmid pJH222). Among 14 rBCG:HIV-1gp120 (pMV261) colonies screened, 12 showed a partial deletion and two showed a complete deletion. However, deletion was not observed in all 10 rBCG:HIV-1gp120 (pJH222) colonies screened. In this study, we demonstrated that E. coli/Mycobacterial expression vectors bearing a weak promoter and lysine complementing gene in a recombinant lysine auxotroph of BCG could prevent genetic rearrangements and disruption of HIV 1gp120 gene expression, a key issue for engineering Mycobacterial based vaccine vectors. PMID:20617151

  17. Distinct Luminal-Type Mammary Carcinomas Arise from Orthotopic Trp53-Null Mammary Transplantation of Juvenile versus Adult Mice

    DOE PAGES

    Nguyen, David H.; Ouyang, Haoxu; Mao, Jian-Hua; ...

    2014-12-01

    Age and physiologic status, such as menopause, are risk factors for breast cancer. Less clear is what factors influence the diversity of breast cancer. In this study, we investigated the effect of host age on the distribution of tumor subtypes in mouse mammary chimera consisting of wild-type hosts and Trp53 nullizygous epithelium, which undergoes a high rate of neoplastic transformation. Wild-type mammary glands cleared of endogenous epithelium at 3 weeks of age were subsequently transplanted during puberty (5 weeks) or at maturation (10 weeks) with syngeneic Trp53-null mammary tissue fragments and monitored for one year. Tumors arose sooner from adultmore » hosts (AH) compared with juvenile hosts (JH). However, compared with AH tumors, JH tumors grew several times faster, were more perfused, exhibited a two-fold higher mitotic index, and were more highly positive for insulin-like growth factor receptor phosphorylation. Most tumors in each setting were estrogen receptor (ER)-positive (80% JH vs. 70% AH), but JH tumors were significantly more ER-immunoreactive (P = 0.0001) than AH tumors. A differential expression signature (JvA) of juvenile versus adult tumors revealed a luminal transcriptional program. Centroids of the human homologs of JvA genes showed that JH tumors were more like luminal A tumors and AH tumors were more like luminal B tumors. Hierarchical clustering with the JvA human ortholog gene list segregated luminal A and luminal B breast cancers across datasets. Lastly, these data support the notion that age-associated host physiology greatly influences the intrinsic subtype of breast cancer.« less

  18. Genetic architecture of a hormonal response to gene knockdown in honey bees.

    PubMed

    Ihle, Kate E; Rueppell, Olav; Huang, Zachary Y; Wang, Ying; Fondrk, M Kim; Page, Robert E; Amdam, Gro V

    2015-01-01

    Variation in endocrine signaling is proposed to underlie the evolution and regulation of social life histories, but the genetic architecture of endocrine signaling is still poorly understood. An excellent example of a hormonally influenced set of social traits is found in the honey bee (Apis mellifera): a dynamic and mutually suppressive relationship between juvenile hormone (JH) and the yolk precursor protein vitellogenin (Vg) regulates behavioral maturation and foraging of workers. Several other traits cosegregate with these behavioral phenotypes, comprising the pollen hoarding syndrome (PHS) one of the best-described animal behavioral syndromes. Genotype differences in responsiveness of JH to Vg are a potential mechanistic basis for the PHS. Here, we reduced Vg expression via RNA interference in progeny from a backcross between 2 selected lines of honey bees that differ in JH responsiveness to Vg reduction and measured JH response and ovary size, which represents another key aspect of the PHS. Genetic mapping based on restriction site-associated DNA tag sequencing identified suggestive quantitative trait loci (QTL) for ovary size and JH responsiveness. We confirmed genetic effects on both traits near many QTL that had been identified previously for their effect on various PHS traits. Thus, our results support a role for endocrine control of complex traits at a genetic level. Furthermore, this first example of a genetic map of a hormonal response to gene knockdown in a social insect helps to refine the genetic understanding of complex behaviors and the physiology that may underlie behavioral control in general. © The American Genetic Association. 2015.

  19. Genetic Architecture of a Hormonal Response to Gene Knockdown in Honey Bees

    PubMed Central

    Rueppell, Olav; Huang, Zachary Y.; Wang, Ying; Fondrk, M. Kim; Page, Robert E.; Amdam, Gro V.

    2015-01-01

    Variation in endocrine signaling is proposed to underlie the evolution and regulation of social life histories, but the genetic architecture of endocrine signaling is still poorly understood. An excellent example of a hormonally influenced set of social traits is found in the honey bee (Apis mellifera): a dynamic and mutually suppressive relationship between juvenile hormone (JH) and the yolk precursor protein vitellogenin (Vg) regulates behavioral maturation and foraging of workers. Several other traits cosegregate with these behavioral phenotypes, comprising the pollen hoarding syndrome (PHS) one of the best-described animal behavioral syndromes. Genotype differences in responsiveness of JH to Vg are a potential mechanistic basis for the PHS. Here, we reduced Vg expression via RNA interference in progeny from a backcross between 2 selected lines of honey bees that differ in JH responsiveness to Vg reduction and measured JH response and ovary size, which represents another key aspect of the PHS. Genetic mapping based on restriction site-associated DNA tag sequencing identified suggestive quantitative trait loci (QTL) for ovary size and JH responsiveness. We confirmed genetic effects on both traits near many QTL that had been identified previously for their effect on various PHS traits. Thus, our results support a role for endocrine control of complex traits at a genetic level. Furthermore, this first example of a genetic map of a hormonal response to gene knockdown in a social insect helps to refine the genetic understanding of complex behaviors and the physiology that may underlie behavioral control in general. PMID:25596612

  20. Distinct Luminal-Type Mammary Carcinomas Arise from Orthotopic Trp53-Null Mammary Transplantation of Juvenile versus Adult Mice

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Nguyen, David H.; Ouyang, Haoxu; Mao, Jian-Hua

    Age and physiologic status, such as menopause, are risk factors for breast cancer. Less clear is what factors influence the diversity of breast cancer. In this study, we investigated the effect of host age on the distribution of tumor subtypes in mouse mammary chimera consisting of wild-type hosts and Trp53 nullizygous epithelium, which undergoes a high rate of neoplastic transformation. Wild-type mammary glands cleared of endogenous epithelium at 3 weeks of age were subsequently transplanted during puberty (5 weeks) or at maturation (10 weeks) with syngeneic Trp53-null mammary tissue fragments and monitored for one year. Tumors arose sooner from adultmore » hosts (AH) compared with juvenile hosts (JH). However, compared with AH tumors, JH tumors grew several times faster, were more perfused, exhibited a two-fold higher mitotic index, and were more highly positive for insulin-like growth factor receptor phosphorylation. Most tumors in each setting were estrogen receptor (ER)-positive (80% JH vs. 70% AH), but JH tumors were significantly more ER-immunoreactive (P = 0.0001) than AH tumors. A differential expression signature (JvA) of juvenile versus adult tumors revealed a luminal transcriptional program. Centroids of the human homologs of JvA genes showed that JH tumors were more like luminal A tumors and AH tumors were more like luminal B tumors. Hierarchical clustering with the JvA human ortholog gene list segregated luminal A and luminal B breast cancers across datasets. Lastly, these data support the notion that age-associated host physiology greatly influences the intrinsic subtype of breast cancer.« less

  1. Hormonal regulation and developmental role of Krüppel homolog 1, a repressor of metamorphosis, in the silkworm Bombyx mori.

    PubMed

    Kayukawa, Takumi; Murata, Mika; Kobayashi, Isao; Muramatsu, Daisuke; Okada, Chieko; Uchino, Keiro; Sezutsu, Hideki; Kiuchi, Makoto; Tamura, Toshiki; Hiruma, Kiyoshi; Ishikawa, Yukio; Shinoda, Tetsuro

    2014-04-01

    Juvenile hormone (JH) has an ability to repress the precocious metamorphosis of insects during their larval development. Krüppel homolog 1 (Kr-h1) is an early JH-inducible gene that mediates this action of JH; however, the fine hormonal regulation of Kr-h1 and the molecular mechanism underlying its antimetamorphic effect are little understood. In this study, we attempted to elucidate the hormonal regulation and developmental role of Kr-h1. We found that the expression of Kr-h1 in the epidermis of penultimate-instar larvae of the silkworm Bombyx mori was induced by JH secreted by the corpora allata (CA), whereas the CA were not involved in the transient induction of Kr-h1 at the prepupal stage. Tissue culture experiments suggested that the transient peak of Kr-h1 at the prepupal stage is likely to be induced cooperatively by JH derived from gland(s) other than the CA and the prepupal surge of ecdysteroid, although involvement of unknown factor(s) could not be ruled out. To elucidate the developmental role of Kr-h1, we generated transgenic silkworms overexpressing Kr-h1. The transgenic silkworms grew normally until the spinning stage, but their development was arrested at the prepupal stage. The transgenic silkworms from which the CA were removed in the penultimate instar did not undergo precocious pupation or larval-larval molt but fell into prepupal arrest. This result demonstrated that Kr-h1 is indeed involved in the repression of metamorphosis but that Kr-h1 alone is incapable of implementing normal larval molt. Moreover, the expression profiles and hormonal responses of early ecdysone-inducible genes (E74, E75, and Broad) in transgenic silkworms suggested that Kr-h1 is not involved in the JH-dependent modulation of these genes, which is associated with the control of metamorphosis. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Potentiation Following Ballistic and Nonballistic Complexes: The Effect of Strength Level.

    PubMed

    Suchomel, Timothy J; Sato, Kimitake; DeWeese, Brad H; Ebben, William P; Stone, Michael H

    2016-07-01

    Suchomel, TJ, Sato, K, DeWeese, BH, Ebben, WP, and Stone, MH. Potentiation following ballistic and nonballistic complexes: the effect of strength level. J Strength Cond Res 30(7): 1825-1833, 2016-The purpose of this study was to compare the temporal profile of strong and weak subjects during ballistic and nonballistic potentiation complexes. Eight strong (relative back squat = 2.1 ± 0.1 times body mass) and 8 weak (relative back squat = 1.6 ± 0.2 times body mass) males performed squat jumps immediately and every minute up to 10 minutes following potentiation complexes that included ballistic or nonballistic concentric-only half-squat (COHS) performed at 90% of their 1 repetition maximum COHS. Jump height (JH) and allometrically scaled peak power (PPa) were compared using a series of 2 × 12 repeated measures analyses of variance. No statistically significant strength level main effects for JH (p = 0.442) or PPa (p = 0.078) existed during the ballistic condition. In contrast, statistically significant main effects for time existed for both JH (p = 0.014) and PPa (p < 0.001); however, no statistically significant pairwise comparisons were present (p > 0.05). Statistically significant strength level main effects existed for PPa (p = 0.039) but not for JH (p = 0.137) during the nonballistic condition. Post hoc analysis revealed that the strong subjects produced statistically greater PPa than the weaker subjects (p = 0.039). Statistically significant time main effects existed for time existed for PPa (p = 0.015), but not for JH (p = 0.178). No statistically significant strength level × time interaction effects for JH (p = 0.319) or PPa (p = 0.203) were present for the ballistic or nonballistic conditions. Practical significance indicated by effect sizes and the relationships between maximum potentiation and relative strength suggest that stronger subjects potentiate earlier and to a greater extent than weaker subjects during ballistic and nonballistic potentiation

  3. Cloning and expressing a highly functional and substrate specific farnesoic acid o-methyltransferase from the Asian citrus psyllid (Diaphorina citri Kuwayama).

    PubMed

    Van Ekert, Evelien; Shatters, Robert G; Rougé, Pierre; Powell, Charles A; Smagghe, Guy; Borovsky, Dov

    2015-01-01

    The Asian citrus psyllid, Diaphorina citri, transmits a phloem-limited bacterium, Candidatus 'Liberibacter' asiaticus that causes citrus greening disease. Because juvenile hormone (JH) plays an important role in adult and nymphal development, we studied the final steps in JH biosynthesis in D. citri. A putative JH acid methyltransferase ortholog gene (jmtD) and its cognate cDNA were identified by searching D. citri genome database. Expression analysis shows expression in all life stages. In adults, it is expressed in the head-thorax, (containing the corpora allata), and the abdomen (containing ovaries and male accessory glands). A 3D protein model identified the catalytic groove with catalytically active amino acids and the S-adenosyl methionine (SAM)-binding loop. The cDNA was expressed in Escherichia coli cells and the purified enzyme showed high preference for farnesoic acid (FA) and homoFA (kcat of 0.752 × 10(-3) and 0.217 × 10(-3) s(-1), respectively) as compared to JH acid I (JHA I) (cis/trans/cis; 2Z, 6E, 10cis), JHA III (2E, 6E, 10cis), and JHA I (trans/cis/cis; 2E, 2Z, 10cis) (kcat of 0.081 × 10(-3), 0.013 × 10(-3), and 0.003 × 10(-3) s(-1), respectively). This suggests that this ortholog is a DcFA-o-methyl transferase gene (fmtD), not a jmtD, and that JH biosynthesis in D. citri proceeds from FA to JH III through methyl farnesoate (MF). DcFA-o-MT does not require Ca(2+), Mg(2+) or Zn(2+), however, Zn(2+) (1 mM) completely inhibits the enzyme probably by binding H115 at the active groove. This represents the first purified FA-o-MT from Hemiptera with preferred biological activity for FA and not JHA.

  4. Endocrine Control of Exaggerated Trait Growth in Rhinoceros Beetles.

    PubMed

    Zinna, R; Gotoh, H; Brent, C S; Dolezal, A; Kraus, A; Niimi, T; Emlen, D; Lavine, L C

    2016-08-01

    Juvenile hormone (JH) is a key insect growth regulator frequently involved in modulating phenotypically plastic traits such as caste determination in eusocial species, wing polymorphisms in aphids, and mandible size in stag beetles. The jaw morphology of stag beetles is sexually-dimorphic and condition-dependent; males have larger jaws than females and those developing under optimum conditions are larger in overall body size and have disproportionately larger jaws than males raised under poor conditions. We have previously shown that large males have higher JH titers than small males during development, and ectopic application of fenoxycarb (JH analog) to small males can induce mandibular growth similar to that of larger males. What remains unknown is whether JH regulates condition-dependent trait growth in other insects with extreme sexually selected structures. In this study, we tested the hypothesis that JH mediates the condition-dependent expression of the elaborate horns of the Asian rhinoceros beetle, Trypoxylus dichotomus. The sexually dimorphic head horn of this beetle is sensitive to nutritional state during larval development. Like stag beetles, male rhinoceros beetles receiving copious food produce disproportionately large horns for their body size compared with males under restricted diets. We show that JH titers are correlated with body size during the late feeding and early prepupal periods, but this correlation disappears by the late prepupal period, the period of maximum horn growth. While ectopic application of fenoxycarb during the third larval instar significantly delayed pupation, it had no effect on adult horn size relative to body size. Fenoxycarb application to late prepupae also had at most a marginal effect on relative horn size. We discuss our results in context of other endocrine signals of condition-dependent trait exaggeration and suggest that different beetle lineages may have co-opted different physiological signaling mechanisms to

  5. Rad50S alleles of the Mre11 complex: questions answered and questions raised.

    PubMed

    Usui, Takehiko; Petrini, John H J; Morales, Monica

    2006-08-15

    We find that Rad50S mutations in yeast and mammals exhibit constitutive PIKK (PI3-kinase like kinase)-dependent signaling [T. Usui, H. Ogawa, J.H. Petrini, A DNA damage response pathway controlled by Tel1 and the Mre11 complex. Mol. Cell 7 (2001) 1255-1266.; M. Morales, J.W. Theunissen, C.F. Kim, R. Kitagawa, M.B. Kastan, J.H. Petrini, The Rad50S allele promotes ATM-dependent DNA damage responses and suppresses ATM deficiency: implications for the Mre11 complex as a DNA damage sensor. Genes Dev. 19 (2005) 3043-4354.]. The signaling depends on Mre11 complex functions, consistent with its role as a DNA damage sensor. Rad50S is distinct from hypomorphic mutations of Mre11 and Nbs1 in mammals [M. Morales, J.W. Theunissen, C.F. Kim, R. Kitagawa, M.B. Kastan, J.H. Petrini, The Rad50S allele promotes ATM-dependent DNA damage responses and suppresses ATM deficiency: implications for the Mre11 complex as a DNA damage sensor. Genes Dev. 19 (2005) 3043-3054.; J.P. Carney, R.S. Maser, H. Olivares, E.M. Davis, Le M. Beau, J.R. Yates, III, L. Hays, W.F. Morgan, J.H. Petrini, The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. Cell 93 (1998) 477-486.; G.S. Stewart, R.S. Maser, T. Stankovic, D.A. Bressan, M.I. Kaplan, N.G. Jaspers, A. Raams, P.J. Byrd, J.H. Petrini, A.M. Taylor, The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia-telangiectasia-like disorder. Cell 99 (1999) 577-587.; B.R. Williams, O.K. Mirzoeva, W.F. Morgan, J. Lin, W. Dunnick, J.H. Petrini, A murine model of nijmegen breakage syndrome. Curr. Biol. 12 (2002) 648-653.; J.W. Theunissen, M.I. Kaplan, P.A. Hunt, B.R. Williams, D.O. Ferguson, F.W. Alt, J.H. Petrini, Checkpoint failure and chromosomal instability without lymphomagenesis in Mre11(ATLD1/ATLD1) mice. Mol. Cell 12 (2003) 1511-1523.] and the Mre11 complex deficiency in yeast [T. Usui, H. Ogawa, J.H. Petrini, A DNA damage response

  6. Department of Clinical Investigation Annual Research Progress Report, Fiscal Year 1989

    DTIC Science & Technology

    1989-09-30

    and DES. Patients with a history of deep vein thrombosis, cerebral embolus, stroke , congestive heart failure, or ischemic heart disease will not be...Adolescent Health Care 9(6): 498-500, 1988 Weir MR Intravascular Injuries from Intramuscular Penicillin. Clinical Pediatrics 27(2): 85-90, 1988 Weir...Military Medicine 154(9): 455-57, 1989 Piatt JH, Goodkin R Barbiturates in Severe Head Injury . J Neurosurgery 70(3): 501-02, 1989 * Piatt JH

  7. Biodegradable Bioadherent Microcapsules for Orally Administered Sustained Release Vaccines

    DTIC Science & Technology

    1997-05-01

    Fasciola hepatica which is a known bioadhesive’’. The encapsulation method is the classical, well described water in oil technique for the preparation of...immunization, Vaccine 12 (1994) 387-340. 6. Waite, J.H., Rice-Ficht, A.C., Presclerotized eggshell protein from the liver fluke Fasciola hepatica...Biochemistry 26 (1987) 7819-7825. 7. Waite, J.H., Rice-Ficht, A.C., Eggshell precursor proteins of Fasciola hepatica: II. Microheterogeneity in vitelline

  8. Reproductive status, endocrine physiology and chemical signaling in the Neotropical, swarm-founding eusocial wasp Polybia micans

    PubMed Central

    Kelstrup, Hans C.; Hartfelder, Klaus; Nascimento, Fabio S.; Riddiford, Lynn M.

    2014-01-01

    In the evolution of caste-based societies in Hymenoptera, the classical insect hormones juvenile hormone (JH) and ecdysteroids were co-opted into new functions. Social wasps, which show all levels of sociality and lifestyles, are an ideal group in which to study such functional changes. Virtually all studies on the physiological mechanisms underlying reproductive division of labor and caste functions in wasps have been done on independent-founding paper wasps, and the majority of these studies have focused on species specially adapted for overwintering. The relatively little-studied tropical swarm-founding wasps of the Epiponini (Vespidae) are a diverse group of permanently social wasps, with some species maintaining caste flexibility well into the adult phase. We investigated the behavior, reproductive status, JH and ecdysteroid titers in hemolymph, ecdysteroid content of the ovary and cuticular hydrocarbon (CHC) profiles in the caste-monomorphic, epiponine wasp Polybia micans Ducke. We found that the JH titer was not elevated in competing queens from established multiple-queen nests, but increased in lone queens that lack direct competition. In queenless colonies, JH titer rose transiently in young potential reproductives upon challenge by nestmates, suggesting that JH may prime the ovaries for further development. Ovarian ecdysteroids were very low in workers but higher and correlated with the number of vitellogenic oocytes in the queens. Hemolymph ecdysteroid levels were low and variable in both workers and queens. Profiles of P. micans CHCs reflected caste, age and reproductive status, but were not tightly linked to either hormone. These findings show a significant divergence in hormone function in swarm-founding wasps compared with independently founding ones. PMID:24744417

  9. Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance.

    PubMed

    Ni, Mi; Ma, Wei; Wang, Xiaofang; Gao, Meijing; Dai, Yan; Wei, Xiaoli; Zhang, Lei; Peng, Yonggang; Chen, Shuyuan; Ding, Lingyun; Tian, Yue; Li, Jie; Wang, Haiping; Wang, Xiaolin; Xu, Guowang; Guo, Wangzhen; Yang, Yihua; Wu, Yidong; Heuberger, Shannon; Tabashnik, Bruce E; Zhang, Tianzhen; Zhu, Zhen

    2017-09-01

    Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  10. Is Vertical Jump Height an Indicator of Athletes' Power Output in Different Sport Modalities?

    PubMed

    Kons, Rafael L; Ache-Dias, Jonathan; Detanico, Daniele; Barth, Jonathan; Dal Pupo, Juliano

    2018-03-01

    Kons, RL, Ache-Dias, J, Detanico, D, Barth, J, and Dal Pupo, J. Is vertical jump height an indicator of athletes' power output in different sports modalities? J Strength Cond Res 32(3): 708-715, 2018-This study aimed to identify whether the ratio standard is adequate for the scaling of peak power output (PPO) for body mass (BM) in athletes of different sports and to verify classification agreement for athletes involved in different sports using PPO scaled for BM and jump height (JH). One hundred and twenty-four male athletes divided into 3 different groups-combat sports, team sports, and runners-participated in this study. Participants performed the countermovement jump on a force plate. Peak power output and JH were calculated from the vertical ground reaction force. We found different allometric exponents for each modality, allowing the use of the ratio standard for team sports. For combat sports and runners, the ratio standard was not considered adequate, and therefore, a specific allometric exponent for these 2 groups was found. Significant correlations between adjusted PPO for BM (PPOADJ) and JH were found for all modalities, but it was higher for runners (r = 0.81) than team and combat sports (r = 0.63 and 0.65, respectively). Moderate agreement generated by the PPOADJ and JH was verified in team sports (k = 0.47) and running (k = 0.55) and fair agreement in combat sports (k = 0.29). We conclude that the ratio standard seems to be suitable only for team sports; for runners and combat sports, an allometric model seems adequate. The use of JH as an indicator of power output may be considered reasonable only for runners.

  11. Evidence for a Phe-Gly-Leu-amide-like allatostatin in the beetle Tenebrio molitor.

    PubMed

    Elliott, Karen L; Chan, Kuen Kuen; Stay, Barbara

    2010-03-01

    The allatostatins (ASTs) with Phe-Gly-Leu-amide C-terminal sequence are multifunctional neuropeptides discovered as inhibitors of juvenile hormone (JH) synthesis by corpora allata (CA) of cockroaches. Although these ASTs inhibit JH synthesis only in cockroaches, crickets, termites and locusts, isolation of peptides or of cDNA/genomic DNA or analysis of genomes indicates their occurrence in many orders of insects with the exception of coleopterans. The gene for these ASTs has not been found in the genome of the red flour beetle Tribolium castaneum (Family Tenebrionidae). Yet, in view of widespread occurrence of these peptides in insects, crustaceans and nematodes, they would be expected to occur in beetles. This study provides evidence for the presence of FGLa-like ASTs in the tenebrionid beetle, Tenebrio molitor, and scarabid beetle, Popillia japonica. Extract of brain from both beetles inhibited JH synthesis by cockroach CA dose dependently and reversibly. 20 brain equivalents of T. molitor and P. japonica extracts inhibited JH synthesis 64+/-5 and 65+/-0.6% respectively. Antibody against cockroach allatostatin (Diploptera punctata AST-7) used in an enzyme-linked immunosorbent assay reacted with brain extract of these beetles. Antibody against D. punctata AST-5 localized FGLa-like ASTs in the brain and subesophageal ganglion of T. molitor and P. japonica. In addition, pretreatment of T. molitor brain extract with anti-D. punctata AST-5 reduced the inhibition of JH synthesis and pretreatment of anti-D. punctata AST-5 with D. punctata AST-5 diminished the immunoreactivity of the antibody. Thus we predict that FGLa-like allatostatins will be found in beetles. (c) 2009 Elsevier Inc. All rights reserved.

  12. Streptosporangium jiaoheense sp. nov. and Streptosporangium taraxaci sp. nov., actinobacteria isolated from soil and dandelion root (Taraxacum mongolicum Hand.-Mazz.).

    PubMed

    Zhao, Junwei; Guo, Lifeng; Li, Zhilei; Piao, Chenyu; Li, Yao; Li, Jiansong; Liu, Chongxi; Wang, Xiangjing; Xiang, Wensheng

    2016-06-01

    Two novel actinobacteria, designated strains NEAU-Jh1-4T and NEAU-Wp2-0T, were isolated from muddy soil collected from a riverbank in Jiaohe and a dandelion root collected from Harbin, respectively. A polyphasic study was carried out to establish the taxonomic positions of these two strains. The phylogenetic analysis based on the 16S rRNA gene sequences of strains NEAU-Jh1-4T and NEAU-Wp2-0T indicated that strain NEAU-Jh1-4T clustered with Streptosporangium nanhuense NEAU-NH11T (99.32 % 16S rRNA gene sequence similarity), Streptosporangium purpuratum CY-15110T (98.30 %) and Streptosporangium yunnanense CY-11007T (97.95 %) and strain NEAU-Wp2-0T clustered with 'Streptosporangium sonchi  ' NEAU-QS7 (99.39 %), 'Streptosporangium kronopolitis' NEAU-ML10 (99.26 %), 'Streptosporangium shengliense' NEAU-GH7 (98.85 %) and Streptosporangium longisporum DSM 43180T (98.69 %). Moreover, morphological and chemotaxonomic properties of the two isolates also confirmed their affiliation to the genus Streptosporangium. However, the low level of DNA-DNA hybridization and some phenotypic characteristics allowed the isolates to be differentiated from the most closely related species. Therefore, it is proposed that strains NEAU-Jh1-4T and NEAU-Wp2-0T represent two novel species of the genus Streptosporangium, for which the name Streptosporangium jiaoheense sp. nov. and Streptosporangium taraxaci sp. nov. are proposed. The type strains are NEAU-Jh1-4T (=CGMCC 4.7213T=JCM 30348T) and NEAU-Wp2-0T (=CGMCC 4.7217T=JCM 30349T), respectively.

  13. Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors

    PubMed Central

    Spit, Jornt; Holtof, Michiel; Badisco, Liesbet; Vergauwen, Lucia; Vogel, Elise; Knapen, Dries; Vanden Broeck, Jozef

    2016-01-01

    Herbivorous insects evolved adaptive mechanisms to compensate for the presence of plant defensive protease inhibitors (PI) in their food. The underlying regulatory mechanisms of these compensatory responses remain largely elusive. In the current study, we investigated the initiation of this adaptive response in the migratory locust, Locusta migratoria, via microarray analysis of gut tissues. Four hours after dietary uptake of PIs, 114 and 150 transcripts were respectively found up- or downregulated. The results suggest a quick trade-off between compensating for potential loss of digestive activity on the one hand, and stress tolerance, defense, and structural integrity of the gut on the other hand. We additionally addressed the role of a group of related upregulated hexamerin-like proteins in the PI-induced response. Simultaneous knockdown of corresponding transcripts by means of RNA interference resulted in a reduced capacity of the locust nymphs to cope with the effects of PI. Moreover, since insect hexamerins have been shown to bind Juvenile Hormone (JH), we also investigated the effect of JH on the proteolytic digestion in L. migratoria. Our results indicate that JH has a stimulatory effect on the expression of three homologous chymotrypsin genes, while knocking down the JH receptor (methoprene tolerant) led to opposite effects. PMID:27581362

  14. Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors

    NASA Astrophysics Data System (ADS)

    Spit, Jornt; Holtof, Michiel; Badisco, Liesbet; Vergauwen, Lucia; Vogel, Elise; Knapen, Dries; vanden Broeck, Jozef

    2016-09-01

    Herbivorous insects evolved adaptive mechanisms to compensate for the presence of plant defensive protease inhibitors (PI) in their food. The underlying regulatory mechanisms of these compensatory responses remain largely elusive. In the current study, we investigated the initiation of this adaptive response in the migratory locust, Locusta migratoria, via microarray analysis of gut tissues. Four hours after dietary uptake of PIs, 114 and 150 transcripts were respectively found up- or downregulated. The results suggest a quick trade-off between compensating for potential loss of digestive activity on the one hand, and stress tolerance, defense, and structural integrity of the gut on the other hand. We additionally addressed the role of a group of related upregulated hexamerin-like proteins in the PI-induced response. Simultaneous knockdown of corresponding transcripts by means of RNA interference resulted in a reduced capacity of the locust nymphs to cope with the effects of PI. Moreover, since insect hexamerins have been shown to bind Juvenile Hormone (JH), we also investigated the effect of JH on the proteolytic digestion in L. migratoria. Our results indicate that JH has a stimulatory effect on the expression of three homologous chymotrypsin genes, while knocking down the JH receptor (methoprene tolerant) led to opposite effects.

  15. Cloning and sequence analysis of Galleria mellonella juvenile hormone binding protein--a search for ancestors and relatives.

    PubMed

    Rodriguez Parkitna, Jan M; Ozyhar, Andrzej; Wiśniewski, Jacek R; Kochman, Marian

    2002-09-01

    Juvenile hormone binding proteins (JHBPs) serve as specific carriers of juvenile hormone (JH) in insect hemolymph. As shown in this report, Galleria mellonella JHBP is encoded by a cDNA of 1063 nucleotides. The pre-protein consists of 245 amino acids with a 20 amino acid leader sequence. The concentration of the JHBP mRNA reaches a maximum on the third day of the last larval instar, and decreases five-fold towards pupation. Comparison of amino acid sequences of JHBPs from Bombyx mori, Heliothis virescens, Manduca sexta and G. mellonella shows that 57 positions out of 226 are occupied by identical amino acids. A phylogeny tree was constructed from 32 proteins, which function could be associated to JH. It has three major branches: (i) ligand binding domains of nuclear receptors, (ii) JHBPs and JH esterases (JHEs), and (iii) hypothetical proteins found in Drosophila melanogaster genome. Despite the close positioning of JHEs and JHBPs on the tree, which probably arises from the presence of a common JH binding motif, these proteins are unlikely to belong to the same family. Detailed analysis of the secondary structure modeling shows that JHBPs may contain a beta-barrel motif flanked by alpha-helices and thus be evolutionary related to the same superfamily as calycins.

  16. Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors.

    PubMed

    Spit, Jornt; Holtof, Michiel; Badisco, Liesbet; Vergauwen, Lucia; Vogel, Elise; Knapen, Dries; Vanden Broeck, Jozef

    2016-09-01

    Herbivorous insects evolved adaptive mechanisms to compensate for the presence of plant defensive protease inhibitors (PI) in their food. The underlying regulatory mechanisms of these compensatory responses remain largely elusive. In the current study, we investigated the initiation of this adaptive response in the migratory locust, Locusta migratoria, via microarray analysis of gut tissues. Four hours after dietary uptake of PIs, 114 and 150 transcripts were respectively found up- or downregulated. The results suggest a quick trade-off between compensating for potential loss of digestive activity on the one hand, and stress tolerance, defense, and structural integrity of the gut on the other hand. We additionally addressed the role of a group of related upregulated hexamerin-like proteins in the PI-induced response. Simultaneous knockdown of corresponding transcripts by means of RNA interference resulted in a reduced capacity of the locust nymphs to cope with the effects of PI. Moreover, since insect hexamerins have been shown to bind Juvenile Hormone (JH), we also investigated the effect of JH on the proteolytic digestion in L. migratoria. Our results indicate that JH has a stimulatory effect on the expression of three homologous chymotrypsin genes, while knocking down the JH receptor (methoprene tolerant) led to opposite effects.

  17. Reproductive Cost Associated With Juvenile Hormone in Bt-Resistant Strains of Helicoverpa armigera (Lepidoptera: Noctuidae).

    PubMed

    Zhang, W N; Ma, L; Wang, B J; Chen, L; Khaing, M M; Lu, Y H; Liang, G M; Guo, Y Y

    2016-12-01

    Transgenic Bacillus thuringiensis (Bt) crops are increasingly significant in pest control, but resistance development of target pests is a major issue in the sustainable deployment of Bt crops. The fitness cost of resistance in target pests is regarded as one of the main factors delaying resistance when adopting the refuge strategy. In this study, we compared the life-history traits of three independent sets of Helicoverpa armigera (Hübner, 1809) adults, of each there were a susceptible population and a Cry1Ac-resistant population derived by selection from it. Confirming to the previous studies, resistant individuals exhibited fewer progeny, less fecundity, lower egg hatching rate, and longer adult longevity. And poor fecundity in resistant strains was associated with the decline of the mature follicular amount, the ovarian weight ratio, and the length of the longest ovarian tubule. Interestingly, the juvenile hormone (JH) level appeared higher in resistant strains relative to susceptible strains. Application of methoprene (JH analogue) in vivo was effective in reducing fecundity and hatchability with the up-regulation of detected JH titer. These results suggested that resistance against Bt toxin reduced the reproductive capacity of H. armigera, and JH level is affected in the tradeoff between reproductive capacity and Bt resistance. © Crown copyright 2016.

  18. Electrogenic active proton pump in Rana esculenta skin and its role in sodium ion transport.

    PubMed

    Ehrenfeld, J; Garcia-Romeu, F; Harvey, B J

    1985-02-01

    Kinetic and electrophysiological studies were carried out in the in vitro Rana esculenta skin, bathed in dilute sodium solution, to characterize the proton pump and coupling between sodium absorption (JNa+n) and proton excretion (JH+n). JNa+n and JH+n were both dependent on transepithelial potential (psi ms); hyperpolarizing the skin decreased JNa+n and increased JH+n; depolarization produced the opposite effects. Amiloride (5 X 10(-5) M) at a clamped psi ms of +50 mV inhibited JNa+n without affecting JH+n. Variations of psi ms or pH had identical effects on JH+n. Ethoxzolamide inhibited JH+n and simultaneously increased psi ms by 15-30 mV. These changes were accompanied by depolarization of the apical membrane potential psi mc from -47 to -25 mV and an increase in apical membrane resistance of 30%; no significant effects on basolateral membrane potential (psi cs) and resistance (Rb) nor on shunt resistance (Rj) were observed. The proton pump appears to be localized at the apical membrane. The proton pump was also inhibited by deoxygenation, oligomycin, dicyclohexylcarbodiimide and vanadate (100, 78, 83 and 100% inhibition respectively). The variations of JH+n and of the measured electrical currents were significantly correlated. These findings are supportive evidence of a primary active proton pump, electrogenic and strictly linked to aerobic metabolism. The current-voltage (I-V) relation of the proton pump was obtained as the difference in the I-V curves of the apical membrane extracted before and after proton-pump inhibition by ethoxzolamide during amiloride block of sodium transport. The proton-pump current (IP) was best described by a saturable exponential function of psi mc. Maximal pump current (ImaxP) was calculated to be 200 nequiv h-1 cm-2 at a psi mc of +50 mV and the pump reversal potential ERP was -130 mV. The effect of ethoxzolamide to depolarize psi mc was dependent on the relation between psi mc and ERP. Maximal induced depolarization occurred at a

  19. Electrogenic active proton pump in Rana esculenta skin and its role in sodium ion transport.

    PubMed Central

    Ehrenfeld, J; Garcia-Romeu, F; Harvey, B J

    1985-01-01

    Kinetic and electrophysiological studies were carried out in the in vitro Rana esculenta skin, bathed in dilute sodium solution, to characterize the proton pump and coupling between sodium absorption (JNa+n) and proton excretion (JH+n). JNa+n and JH+n were both dependent on transepithelial potential (psi ms); hyperpolarizing the skin decreased JNa+n and increased JH+n; depolarization produced the opposite effects. Amiloride (5 X 10(-5) M) at a clamped psi ms of +50 mV inhibited JNa+n without affecting JH+n. Variations of psi ms or pH had identical effects on JH+n. Ethoxzolamide inhibited JH+n and simultaneously increased psi ms by 15-30 mV. These changes were accompanied by depolarization of the apical membrane potential psi mc from -47 to -25 mV and an increase in apical membrane resistance of 30%; no significant effects on basolateral membrane potential (psi cs) and resistance (Rb) nor on shunt resistance (Rj) were observed. The proton pump appears to be localized at the apical membrane. The proton pump was also inhibited by deoxygenation, oligomycin, dicyclohexylcarbodiimide and vanadate (100, 78, 83 and 100% inhibition respectively). The variations of JH+n and of the measured electrical currents were significantly correlated. These findings are supportive evidence of a primary active proton pump, electrogenic and strictly linked to aerobic metabolism. The current-voltage (I-V) relation of the proton pump was obtained as the difference in the I-V curves of the apical membrane extracted before and after proton-pump inhibition by ethoxzolamide during amiloride block of sodium transport. The proton-pump current (IP) was best described by a saturable exponential function of psi mc. Maximal pump current (ImaxP) was calculated to be 200 nequiv h-1 cm-2 at a psi mc of +50 mV and the pump reversal potential ERP was -130 mV. The effect of ethoxzolamide to depolarize psi mc was dependent on the relation between psi mc and ERP. Maximal induced depolarization occurred at a

  20. Outcome of Hip Impingement Surgery: Does Generalized Joint Hypermobility Matter?

    PubMed

    Naal, Florian D; Müller, Aileen; Varghese, Viju D; Wellauer, Vanessa; Impellizzeri, Franco M; Leunig, Michael

    2017-05-01

    Generalized joint hypermobility (JH) might negatively influence the results of surgical femoroacetabular impingement (FAI) treatment, as JH has been linked to musculoskeletal pain and injury incidence in athletes. JH may also be associated with worse outcomes of FAI surgery in thin females. To (1) determine the results of FAI surgery at a minimum 2-year follow-up by means of patient-reported outcome measures (PROMs) and failure rates, (2) assess the prevalence of JH in FAI patients and its effect on outcomes, and (3) identify other risk factors associated with treatment failure. Cohort study; Level of evidence, 3. We included 232 consecutive patients (118 females; mean age, 36 years) with 244 hips surgically treated for symptomatic FAI between 2010 and 2012. All patients completed different PROMs preoperatively and at a mean follow-up of 3.7 years. Satisfaction questions were used to define subjective failure (answering any of the 2 subjective questions with dissatisfied/ very dissatisfied and/or didn't help/ made things worse). Conversion to total hip replacement (THR) was defined as objective failure. JH was assessed using the Beighton score. All PROM values significantly ( P < .001) improved from preoperative measurement to follow-up (Oxford Hip Score: 33.8 to 42.4; University of California at Los Angeles Activity Scale: 6.3 to 7.3; EuroQol-5 Dimension Index: 0.58 to 0.80). Overall, 34% of patients scored ≥4 on the Beighton score, and 18% scored ≥6, indicating generalized JH. Eleven hips (4.7%) objectively failed and were converted to THR. Twenty-four patients (10.3%) were considered as subjective failures. No predictive risk factors were identified for subjective failure. Tönnis grade significantly ( P < .001) predicted objective failure (odds ratio, 13; 95% CI, 4-45). There was a weak inverse association ( r = -0.16 to -0.30) between Beighton scores and preoperative PROM values. There were no significant associations between Beighton scores and

  1. The Nanophysics of Electron Emission and Breakdown for High Power Microwave Source

    DTIC Science & Technology

    2009-12-21

    Professor John E. Scharer, Professor Dane Morgan, Professor Xin He, Graduate Student Nishant Sule, Graduate Student Vasilios Vlahos , Graduate...A231-A260 (2005), invited. 52. Vlahos , V., Booske, J.H., Morgan, D.D., “The Effects of Thin CsI coatings on the Work Function of Graphite Cathodes...using Ab-initio Modeling,” Appl. Phys. Lett. 91, paper 144102, 3 pages (2007). 53. Vlahos , V., Lee, Y.-L., Booske, J.H., Morgan, D., Turek, L

  2. The Development and Study of Molecular Electronic Switches and their Field-Effect Transistor (FET) Device Properties

    DTIC Science & Technology

    2015-02-27

    Grand Rapids, MI) meetings and conferences. References 1. L.N Lucas, J.J.D. de Jong, J.H. van Esch, R.M. Kellogg , B.L. Berringa. Eur. J. Org...R.M. Kellogg , H.L. Feringa, H.L. Eur. J. Org. Chem., 2003, 155-166. 6. B. Qin, R. Yao, X. Zhao, H. Tian. Org. Biomol. Chem., 1, 2003, 2187-2191...7. L.N. Lucas, J.H. van Euch, R.M. Kellogg , B. Feringa. Chem. Comm., 1998, 2313-2314.

  3. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    The J.H. Baxter site is in Weed Siskiyou County, north-central California, and consists of the 33-acre J.H. Baxter facility and the adjacent 870-acre Roseburg Forest Products facility. Since 1937, wood treatment operations at the site have involved a variety of chemicals including ammonical copper-zinc-arsenate, creosote, and PCP. The primary contaminants of concern affecting the soil, sediment, ground water, and surface water are organics including PAHs and dioxins; and metals including arsenic.

  4. Gustatory perception and fat body energy metabolism are jointly affected by vitellogenin and juvenile hormone in honey bees.

    PubMed

    Wang, Ying; Brent, Colin S; Fennern, Erin; Amdam, Gro V

    2012-06-01

    Honey bees (Apis mellifera) provide a system for studying social and food-related behavior. A caste of workers performs age-related tasks: young bees (nurses) usually feed the brood and other adult bees inside the nest, while older bees (foragers) forage outside for pollen, a protein/lipid source, or nectar, a carbohydrate source. The workers' transition from nursing to foraging and their foraging preferences correlate with differences in gustatory perception, metabolic gene expression, and endocrine physiology including the endocrine factors vitellogenin (Vg) and juvenile hormone (JH). However, the understanding of connections among social behavior, energy metabolism, and endocrine factors is incomplete. We used RNA interference (RNAi) to perturb the gene network of Vg and JH to learn more about these connections through effects on gustation, gene transcripts, and physiology. The RNAi perturbation was achieved by single and double knockdown of the genes ultraspiracle (usp) and vg, which encode a putative JH receptor and Vg, respectively. The double knockdown enhanced gustatory perception and elevated hemolymph glucose, trehalose, and JH. We also observed transcriptional responses in insulin like peptide 1 (ilp1), the adipokinetic hormone receptor (AKHR), and cGMP-dependent protein kinase (PKG, or "foraging gene" Amfor). Our study demonstrates that the Vg-JH regulatory module controls changes in carbohydrate metabolism, but not lipid metabolism, when worker bees shift from nursing to foraging. The module is also placed upstream of ilp1, AKHR, and PKG for the first time. As insulin, adipokinetic hormone (AKH), and PKG pathways influence metabolism and gustation in many animals, we propose that honey bees have conserved pathways in carbohydrate metabolism and conserved connections between energy metabolism and gustatory perception. Thus, perhaps the bee can make general contributions to the understanding of food-related behavior and metabolic disorders.

  5. Role of juvenile hormone and allatotropin on nutrient allocation, ovarian development and survivorship in mosquitoes

    PubMed Central

    Hernández-Martínez, Salvador; Mayoral, Jaime G.; Li, Yiping; Noriega, Fernando G.

    2009-01-01

    Teneral reserves are utilized to initiate previtellogenic ovarian development in mosquitoes. Females having emerged with low teneral reserves have reduced juvenile hormone (JH) synthesis and previtellogenic development. We investigated what role JH, allatotropin (AT) and other head-factors play in the regulation of previtellogenic ovarian development and adult survivorship. Factors from the head are essential for corpora allata (CA) activation and reproductive maturation. We have shown that decapitation of females within 9–12 h after adult ecdysis prevented normal development of the previtellogenic follicles; however maximum previtellogenic ovarian development could be induced in decapitated females by topically applying a JH analog. When females were decapitated 12 or more hours after emergence nutritional resources had been committed to ovarian development and survivorship was significantly reduced. To study if allatotropin levels correlated with teneral reserves, we measured AT titers in the heads of two adult phenotypes (large and small females) generated by raising larvae under different nutritional diets. In large mosquitoes AT levels increased to a maximum of 45 fmol in day 4; in contrast, the levels of allatotropin in the heads of small mosquitoes remained below 9 fmol during the 7 days evaluated. These results suggest that only when nutrients are appropriate, factors released from the brain induce the CA to synthesize enough JH to activate reproductive maturation. PMID:17070832

  6. Nutrition-dependent fertility response to juvenile hormone in non-social Euodynerus foraminatus wasps and the evolutionary origin of sociality.

    PubMed

    Tibbetts, Elizabeth A; Mettler, Alexander; Donajkowski, Kellie

    2013-03-01

    The reproductive ground plan hypothesis (RGPH) proposes that the ovarian cycle in solitary insects provides the basis for social evolution, so similar mechanisms are predicted to influence reproductive plasticity in social and solitary species. Specifically, reproductive plasticity in social species originated via modification of nutrition-dependent fertility response to juvenile hormone (JH) in solitary insects. Testing this prediction requires information about the factors that influence fertility in non-social relatives of the eusocial hymenoptera. However, no previous studies have examined how JH or nutritional condition influence fertility in Eumenines, the non-social group most closely related to social wasps. Here, we find support for the RGPH, as JH increases Euodynerus foraminatus fertility. Fertility is also condition-dependent, as heavy E. foraminatus are more fertile than light E. foraminatus. In addition, we measure the factors associated with mating success in E. foraminatus, finding that multiple factors influence mating success, including male weight, male mating experience, and female age. There is also higher variance in male than female reproductive success, suggesting that males may experience substantial sexual selection in this species. Overall, the relationships between JH, body weight, and fertility in E. foraminatus support the RGPH for the origin of sociality by demonstrating that there are strong parallels in the mechanisms that mediate fertility of social and non-social wasps. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. Data-driven Techniques to Estimate Parameters in the Homogenized Energy Model for Shape Memory Alloys

    DTIC Science & Technology

    2011-11-01

    sensor. volume 79781K. Proceedings of the SPIE 7978, 2011. [9] D.J. Hartl , D.C. Lagoudas, F.T. Calkins, and J.H. Mabe . Use of a ni60ti shape memory...alloy for active jet engine chevron application: I. thermomechanical characterization. Smart Materials and Structures, 19:1–14, 2010. [10] D.J. Hartl ...D.C. Lagoudas, F.T. Calkins, and J.H. Mabe . Use of a ni60ti shape memory alloy for active jet engine chevron application: II. experimentally validated

  8. The 3D Structure of the Immunoglobulin Heavy-Chain Locus: Implications for Long-Range Genomic Interactions

    PubMed Central

    Jhunjhunwala, Suchit; van Zelm, Menno C.; Peak, Mandy M.; Cutchin, Steve; Riblet, Roy; van Dongen, Jacques J.M.; Grosveld, Frank G.; Knoch, Tobias A.; Murre, Cornelis

    2009-01-01

    SUMMARY The immunoglobulin heavy-chain (Igh) locus is organized into distinct regions that contain multiple variable (VH), diversity (DH), joining (JH) and constant (CH) coding elements. How the Igh locus is structured in 3D space is unknown. To probe the topography of the Igh locus, spatial distance distributions were determined between 12 genomic markers that span the entire Igh locus. Comparison of the distance distributions to computer simulations of alternative chromatin arrangements predicted that the Igh locus is organized into compartments containing clusters of loops separated by linkers. Trilateration and triple-point angle measurements indicated the mean relative 3D positions of the VH, DH, JH, and CH elements, showed compartmentalization and striking conformational changes involving VH and DH-JH elements during early B cell development. In pro-B cells, the entire repertoire of VH regions (2 Mbp) appeared to have merged and juxtaposed to the DH elements, mechanistically permitting long-range genomic interactions to occur with relatively high frequency. PMID:18423198

  9. Downregulation of vitellogenin gene activity increases the gustatory responsiveness of honey bee workers (Apis mellifera)

    PubMed Central

    Amdam, Gro V.; Norberg, Kari; Page, Robert E.; Erber, Joachim; Scheiner, Ricarda

    2008-01-01

    In the honey bee (Apis mellifera), young workers usually perform tasks in the nest while older workers forage in the field. The behavioral shift from nest-task to foraging activity is accompanied by physiological and sensory changes so that foragers can be characterized by a higher juvenile hormone (JH) level, a lower vitellogenin protein titer, and an increased responsiveness to water and sucrose stimuli. JH was hypothesized to be the key mediator of behavioral development, physiology, and sensory sensitivity in honey bee workers. Recent research, however, has shown that JH is controlled by the hemolymph vitellogenin level, which implies that the fat body specific vitellogenin gene can be a key regulator of behavioral change. Here, we show that downregulation of vitellogenin activity by RNA interference (RNAi) causes an increase in the gustatory responsiveness of worker bees. Our observations suggest that vitellogenin is an important regulator of long-term changes in honey bee behavior. PMID:16466813

  10. Determination of Hund's coupling in 5 d oxides using resonant inelastic x-ray scattering

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yuan, Bo; Clancy, J. P.; Cook, A. M.

    2017-06-01

    We report resonant inelastic x-ray scattering (RIXS) measurements on ordered double-perovskite samples containing Re5+ and Ir5+ with 5d(2) and 5d(4) electronic configurations, respectively. In particular, the observedRIXS spectra of Ba2YReO6 and Sr2MIrO6 (M = Y, Gd) show sharp intra-t(2g) transitions, which can be quantitatively understood using a minimal "atomic" Hamiltonian incorporating spin-orbit coupling. and Hund's coupling J(H). Our analysis yields lambda = 0.38(2) eV with J(H) = 0.26(2) eV for Re5+ and lambda = 0.42(2) eV with J(H) = 0.25(4) eV for Ir5+. Our results provide sharp estimates for Hund's coupling in 5d oxides and suggest that it should bemore » treated on equal footing with spin-orbit interaction in multiorbital 5d transition-metal compounds.« less

  11. Impact of Wetting/Oven-Drying Cycles on the Mechanical and Physical Properties of Birch Plywood

    NASA Astrophysics Data System (ADS)

    Sooru, M.; Kasepuu, K.; Kask, R.; Lille, H.

    2015-11-01

    The objective of this study was to explore some physical and mechanical properties and the dimensional stability of birch (Betula sp.) nine-ply veneers glued with phenol-formaldehyde (PF) after 10 cycles of soaking/oven-drying. The properties to be determined were bending strength (BS), modulus of elasticity in bending (MOE), Janka hardness (JH) and thickness swelling (TS), which were tested according to the European Standards (EN). An analytical equation was used for approximation of the change in the physical and mechanical properties of the samples depending on the number of cycles. It was shown that the values of the studied properties were affected most by the first soaking and drying cycles after which BS and MOE decreased continuously while the values of JH and TS stabilized. After 10 cycles the final values of BS, MOE, JH and TS accounted for 75-81%, 95%, 82% and 98.5% of the initial values, respectively.

  12. Krüppel homolog 1 and E93 mediate Juvenile hormone regulation of metamorphosis in the common bed bug, Cimex lectularius.

    PubMed

    Gujar, Hemant; Palli, Subba Reddy

    2016-05-17

    The common bed bug is an obligate hematophagous parasite of humans. We studied the regulation of molting and metamorphosis in bed bugs with a goal to identify key players involved. qRT-PCR studies on the expression of genes known to be involved in molting and metamorphosis showed high levels of Krüppel homolog 1 [Kr-h1, a transcription factor that plays key roles in juvenile hormone (JH) action] mRNA in the penultimate nymphal stage (N4). However, low levels of Kr-h1 mRNA were detected in the fifth and last nymphal stage (N5). Knockdown of Kr-h1 in N4 resulted in a precocious development of adult structures. Kr-h1 maintains the immature stage by suppressing E93 (early ecdysone response gene) in N4. E93 expression increases during the N5 in the absence of Kr-h1 and promotes the development of adult structures. Knockdown of E93 in N5 results in the formation of supernumerary nymphs. The role of JH in the suppression of adult structures through interaction with Kr-h1 and E93 was also studied by the topical application of JH analog, methoprene, to N5. Methoprene induced Kr-h1 and suppressed E93 and induced formation of the supernumerary nymph. These data show interactions between Kr-h1, E93 and JH in the regulation of metamorphosis in the bed bugs.

  13. Quantitative determination of juvenile hormone III and 20-hydroxyecdysone in queen larvae and drone pupae of Apis mellifera by ultrasonic-assisted extraction and liquid chromatography with electrospray ionization tandem mass spectrometry.

    PubMed

    Zhou, Jinhui; Qi, Yitao; Hou, Yali; Zhao, Jing; Li, Yi; Xue, Xiaofeng; Wu, Liming; Zhang, Jinzhen; Chen, Fang

    2011-09-01

    In this paper, a method for the rapid and sensitive analysis of juvenile hormone III (JH III) and 20-hydroxyecdysone (20E) in queen larvae and drone pupae samples was presented. Ultrasound-assisted extraction provided a significant shortening of the leaching time for the extraction of JH III and 20E and satisfactory sensitivity as compared to the conventional shake extraction procedure. After extraction, determination was carried out by liquid chromatography-tandem mass spectrometry (LC-MS/MS) operating in electrospray ionization positive ion mode via multiple reaction monitoring (MRM) without any clean-up step prior to analysis. A linear gradient consisting of (A) water containing 0.1% formic acid and (B) acetonitrile containing 0.1% formic acid, and a ZORBAX SB-Aq column (100 mm × 2.1 mm, 3.5 μm) were employed to obtain the best resolution of the target analytes. The method was validated for linearity, limit of quantification, recovery, matrix effects, precision and stability. Drone pupae samples were found to contain 20E at concentrations of 18.0 ± 0.1 ng/g (mean ± SD) and JH III was detected at concentrations of 0.20 ± 0.06 ng/g (mean ± SD) in queen larvae samples. This validated method provided some practical information for the actual content of JH III and 20E in queen larvae and drone pupae samples. Copyright © 2011 Elsevier B.V. All rights reserved.

  14. Krüppel homolog 1 and E93 mediate Juvenile hormone regulation of metamorphosis in the common bed bug, Cimex lectularius

    PubMed Central

    Gujar, Hemant; Palli, Subba Reddy

    2016-01-01

    The common bed bug is an obligate hematophagous parasite of humans. We studied the regulation of molting and metamorphosis in bed bugs with a goal to identify key players involved. qRT-PCR studies on the expression of genes known to be involved in molting and metamorphosis showed high levels of Krüppel homolog 1 [Kr-h1, a transcription factor that plays key roles in juvenile hormone (JH) action] mRNA in the penultimate nymphal stage (N4). However, low levels of Kr-h1 mRNA were detected in the fifth and last nymphal stage (N5). Knockdown of Kr-h1 in N4 resulted in a precocious development of adult structures. Kr-h1 maintains the immature stage by suppressing E93 (early ecdysone response gene) in N4. E93 expression increases during the N5 in the absence of Kr-h1 and promotes the development of adult structures. Knockdown of E93 in N5 results in the formation of supernumerary nymphs. The role of JH in the suppression of adult structures through interaction with Kr-h1 and E93 was also studied by the topical application of JH analog, methoprene, to N5. Methoprene induced Kr-h1 and suppressed E93 and induced formation of the supernumerary nymph. These data show interactions between Kr-h1, E93 and JH in the regulation of metamorphosis in the bed bugs. PMID:27185064

  15. IRS and TOR nutrient-signaling pathways act via juvenile hormone to influence honey bee caste fate

    PubMed Central

    Mutti, Navdeep S.; Dolezal, Adam G.; Wolschin, Florian; Mutti, Jasdeep S.; Gill, Kulvinder S.; Amdam, Gro V.

    2011-01-01

    SUMMARY Regardless of genetic makeup, a female honey bee becomes a queen or worker depending on the food she receives as a larva. For decades, it has been known that nutrition and juvenile hormone (JH) signaling determine the caste fate of the individual bee. However, it is still largely unclear how these factors are connected. To address this question, we suppressed nutrient sensing by RNA interference (RNAi)-mediated gene knockdown of IRS (insulin receptor substrate) and TOR (target of rapamycin) in larvae reared on queen diet. The treatments affected several layers of organismal organization that could play a role in the response to differential nutrition between castes. These include transcript profiles, proteomic patterns, lipid levels, DNA methylation response and morphological features. Most importantly, gene knockdown abolished a JH peak that signals queen development and resulted in a worker phenotype. Application of JH rescued the queen phenotype in either knockdown, which demonstrates that the larval response to JH remains intact and can drive normal developmental plasticity even when IRS or TOR transcript levels are reduced. We discuss our results in the context of other recent findings on honey bee caste and development and propose that IRS is an alternative substrate for the Egfr (epidermal growth factor receptor) in honey bees. Overall, our study describes how the interplay of nutritional and hormonal signals affects many levels of organismal organization to build different phenotypes from identical genotypes. PMID:22071189

  16. IRS and TOR nutrient-signaling pathways act via juvenile hormone to influence honey bee caste fate.

    PubMed

    Mutti, Navdeep S; Dolezal, Adam G; Wolschin, Florian; Mutti, Jasdeep S; Gill, Kulvinder S; Amdam, Gro V

    2011-12-01

    Regardless of genetic makeup, a female honey bee becomes a queen or worker depending on the food she receives as a larva. For decades, it has been known that nutrition and juvenile hormone (JH) signaling determine the caste fate of the individual bee. However, it is still largely unclear how these factors are connected. To address this question, we suppressed nutrient sensing by RNA interference (RNAi)-mediated gene knockdown of IRS (insulin receptor substrate) and TOR (target of rapamycin) in larvae reared on queen diet. The treatments affected several layers of organismal organization that could play a role in the response to differential nutrition between castes. These include transcript profiles, proteomic patterns, lipid levels, DNA methylation response and morphological features. Most importantly, gene knockdown abolished a JH peak that signals queen development and resulted in a worker phenotype. Application of JH rescued the queen phenotype in either knockdown, which demonstrates that the larval response to JH remains intact and can drive normal developmental plasticity even when IRS or TOR transcript levels are reduced. We discuss our results in the context of other recent findings on honey bee caste and development and propose that IRS is an alternative substrate for the Egfr (epidermal growth factor receptor) in honey bees. Overall, our study describes how the interplay of nutritional and hormonal signals affects many levels of organismal organization to build different phenotypes from identical genotypes.

  17. Juvenile hormone, but not nutrition or social cues, affects reproductive maturation in solitary alkali bees (Nomia melanderi).

    PubMed

    Kapheim, Karen M; Johnson, Makenna M

    2017-10-15

    Eusocial insect colonies are defined by extreme variation in reproductive activity among castes, but the ancestral conditions from which this variation arose are unknown. Investigating the factors that contribute to variation in reproductive physiology among solitary insects that are closely related to social species can help to fill this gap. We experimentally tested the role of nutrition, juvenile hormone (JH) and social cues on reproductive maturation in solitary alkali bees (Halictidae: Nomia melanderi ). We found that alkali bee females emerge from overwintering with small Dufour's glands and small ovaries, containing oocytes in the early stages of development. Oocyte maturation occurs rapidly, and is staggered between the two ovaries. Lab-reared females reached reproductive maturity without access to mates or nesting opportunities, and many had resorbed oocytes. Initial activation of these reproductive structures does not depend on pollen consumption, though dietary protein or lipids may be necessary for long-term reproductive activity. JH is likely to be a limiting factor in alkali bee reproductive activation, as females treated with JH were more likely to develop mature oocytes and Dufour's glands. Unlike for related social bees, the effects of JH were not suppressed by the presence of older, reproductive females. These results provide valuable insight into the factors that influence reproductive activity in an important native pollinator, and those that may have been particularly influential in the evolution of reproductive castes. © 2017. Published by The Company of Biologists Ltd.

  18. Modeling serotonin uptake in the lung shows endothelial transporters dominate over cleft permeation

    PubMed Central

    Bassingthwaighte, James B.

    2013-01-01

    A four-region (capillary plasma, endothelium, interstitial fluid, cell) multipath model was configured to describe the kinetics of blood-tissue exchange for small solutes in the lung, accounting for regional flow heterogeneity, permeation of cell membranes and through interendothelial clefts, and intracellular reactions. Serotonin uptake data from the Multiple indicator dilution “bolus sweep” experiments of Rickaby and coworkers (Rickaby DA, Linehan JH, Bronikowski TA, Dawson CA. J Appl Physiol 51: 405–414, 1981; Rickaby DA, Dawson CA, and Linehan JH. J Appl Physiol 56: 1170–1177, 1984) and Malcorps et al. (Malcorps CM, Dawson CA, Linehan JH, Bronikowski TA, Rickaby DA, Herman AG, Will JA. J Appl Physiol 57: 720–730, 1984) were analyzed to distinguish facilitated transport into the endothelial cells (EC) and the inhibition of tracer transport by nontracer serotonin in the bolus of injectate from the free uninhibited permeation through the clefts into the interstitial fluid space. The permeability-surface area products (PS) for serotonin via the inter-EC clefts were ∼0.3 ml·g−1·min−1, low compared with the transporter-mediated maximum PS of 13 ml·g−1·min−1 (with Km = ∼0.3 μM and Vmax = ∼4 nmol·g−1·min−1). The estimates of serotonin PS values for EC transporters from their multiple data sets were similar and were influenced only modestly by accounting for the cleft permeability in parallel. The cleft PS estimates in these Ringer-perfused lungs are less than half of those for anesthetized dogs (Yipintsoi T. Circ Res 39: 523–531, 1976) with normal hematocrits, but are compatible with passive noncarrier-mediated transport observed later in the same laboratory (Dawson CA, Linehan JH, Rickaby DA, Bronikowski TA. Ann Biomed Eng 15: 217–227, 1987; Peeters FAM, Bronikowski TA, Dawson CA, Linehan JH, Bult H, Herman AG. J Appl Physiol 66: 2328–2337, 1989) The identification and quantitation of the cleft pathway conductance from these

  19. Feasibility of a wireless gamma probe in radioguided surgery.

    PubMed

    Park, Hye Min; Joo, Koan Sik

    2016-06-21

    Radioguided surgery through the use of a gamma probe is an established practice, and has been widely applied in the case of sentinel lymph node biopsies. A wide range of intraoperative gamma probes is commercially available. The primary characteristics of the gamma probes include their sensitivity, spatial resolution, and energy resolution. We present the results obtained from a prototype of a new wireless gamma probe. This prototype is composed of a 20 mm thick cerium-doped gadolinium aluminum gallium garnet (Ce:GAGG) inorganic scintillation crystal from Furukawa Denshi and a Hamamatsu S12572-100C multi-pixel photon counter equipped with a designed electronics. The measured performance characteristics include the energy resolution, energy linearity, angular aperture, spatial resolution and sensitivity. Measurements were carried out using (57)Co, (133)Ba, (22)Na, and (137)Cs sources. The energy resolutions for 0.122 and 0.511 MeV were 17.2% and 6.9%, respectively. The designed prototype consumes an energy of approximately 4.4 W, weighs about 310 g (including battery) having a dimension of 20 mm (D)  ×  130 mm (L).

  20. Feasibility of a wireless gamma probe in radioguided surgery

    NASA Astrophysics Data System (ADS)

    Park, Hye Min; Joo, Koan Sik

    2016-06-01

    Radioguided surgery through the use of a gamma probe is an established practice, and has been widely applied in the case of sentinel lymph node biopsies. A wide range of intraoperative gamma probes is commercially available. The primary characteristics of the gamma probes include their sensitivity, spatial resolution, and energy resolution. We present the results obtained from a prototype of a new wireless gamma probe. This prototype is composed of a 20 mm thick cerium-doped gadolinium aluminum gallium garnet (Ce:GAGG) inorganic scintillation crystal from Furukawa Denshi and a Hamamatsu S12572-100C multi-pixel photon counter equipped with a designed electronics. The measured performance characteristics include the energy resolution, energy linearity, angular aperture, spatial resolution and sensitivity. Measurements were carried out using 57Co, 133Ba, 22Na, and 137Cs sources. The energy resolutions for 0.122 and 0.511 MeV were 17.2% and 6.9%, respectively. The designed prototype consumes an energy of approximately 4.4 W, weighs about 310 g (including battery) having a dimension of 20 mm (D)  ×  130 mm (L).

  1. Calcium Carbonate Precipitation by Bacillus and Sporosarcina Strains Isolated from Concrete and Analysis of the Bacterial Community of Concrete.

    PubMed

    Kim, Hyun Jung; Eom, Hyo Jung; Park, Chulwoo; Jung, Jaejoon; Shin, Bora; Kim, Wook; Chung, Namhyun; Choi, In-Geol; Park, Woojun

    2016-03-01

    Microbially induced calcium carbonate precipitation (CCP) is a long-standing but re-emerging environmental engineering process for production of self-healing concrete, bioremediation, and long-term storage of CO2. CCP-capable bacteria, two Bacillus strains (JH3 and JH7) and one Sporosarcina strain (HYO08), were isolated from two samples of concrete and characterized phylogenetically. Calcium carbonate crystals precipitated by the three strains were morphologically distinct according to field emission scanning electron microscopy. Energy dispersive X-ray spectrometry mapping confirmed biomineralization via extracellular calcium carbonate production. The three strains differed in their physiological characteristics: growth at alkali pH and high NaCl concentrations, and urease activity. Sporosarcina sp. HYO08 and Bacillus sp. JH7 were more alkali- and halotolerant, respectively. Analysis of the community from the same concrete samples using barcoded pyrosequencing revealed that the relative abundance of Bacillus and Sporosarcina species was low, which indicated low culturability of other dominant bacteria. This study suggests that calcium carbonate crystals with different properties can be produced by various CCP-capable strains, and other novel isolates await discovery.

  2. Evaluation of tunnel seismic prediction (TSP) result using the Japanese highway rock mass classification system for Pahang-Selangor Raw Water Transfer Tunnel

    NASA Astrophysics Data System (ADS)

    Von, W. C.; Ismail, M. A. M.

    2017-10-01

    The knowing of geological profile ahead of tunnel face is significant to minimize the risk in tunnel excavation work and cost control in preventative measure. Due to mountainous area, site investigation with vertical boring is not recommended to obtain the geological profile for Pahang-Selangor Raw Water Transfer project. Hence, tunnel seismic prediction (TSP) method is adopted to predict the geological profile ahead of tunnel face. In order to evaluate the TSP results, IBM SPSS Statistic 22 is used to run artificial neural network (ANN) analysis to back calculate the predicted Rock Grade Points (JH) from actual Rock Grade Points (JH) using Vp, Vs and Vp/Vs from TSP. The results show good correlation between predicted Rock Grade points and actual Rock Grade Points (JH). In other words, TSP can provide geological profile prediction ahead of tunnel face significantly while allowing continuously TBM excavation works. Identifying weak zones or faults ahead of tunnel face is crucial for preventative measures to be carried out in advance for a safer tunnel excavation works.

  3. Cloning and characterization of a riboflavin-binding hexamerin from the larval fat body of a lepidopteran stored grain pest, Corcyra cephalonica.

    PubMed

    Rao, V Venkat; Ningshen, Thuirei Jacob; Chaitanya, R K; Senthilkumaran, B; Dutta-Gupta, Aparna

    2016-01-01

    In the present study, a riboflavin-binding hexamerin (RbHex) was cloned and characterized from the larval fat body of Corcyra cephalonica. The complete cDNA (2121bp) encodes a 706-amino acid protein with a molecular mass ~82kDa. Expression of RbHex 82 was predominant in fat body among larval tissues. Further, it is prominently expressed during the last instar larval development. Homology modeling and docking studies predicted riboflavin binding site of the hexamerin. Spectrofluorimetric analysis further confirmed riboflavin release from the hexamerin fraction. Quantitative RT-PCR studies demonstrated hormonal regulation of RbHex 82. 20-Hydroxyecdysone (20HE) had a stimulatory effect on its transcription whereas JH alone did not show any effect. However, JH in the presence of 20HE maintains the RbHex 82 expression which indicates the JH's role as a status quo factor. This study is the first to report the characterization of riboflavin-binding hexamerin in a lepidopteran pest. Further, the possibility of RbHex 82 as a pest control target is discussed. Copyright © 2016 Elsevier Inc. All rights reserved.

  4. Implications of using broadband photometry for compositional remote sensing of icy objects. [including natural satellites and asteroids

    NASA Technical Reports Server (NTRS)

    Clark, R. N.

    1982-01-01

    The validity and limitations of assuming bright surfaces are icy and dark surfaces are stony are investigated, and the limitations of JHK colorimetry for distinguishing icy versus stony objects are studied. The broadband JHK reflectances of a large range of minerals and mineral assemblages were computed, the visual albedo obtained, and the J-H and H-K colors computed. Visual reflectance was found to vary easily from 0.15 to 1.0 when the surface contains 99 percent or more water by weight. The effect of varying particulate weight fraction and grain size are described. Visual albedo is found to give no indication of the purity of an icy surface. The JHK colors of an ice and particulate mixture can fall anywhere in the classical J-H versus H-K diagram, and thus the diagram cannot be used to distinguish a predominantly rock surface from a predominantly ice one in a specific case, except where both J-H and H-K colors are less than about -0.2.

  5. Atypical ethanol production by carbon catabolite derepressed lactobacilli.

    PubMed

    Kim, Jae-Han; Block, David E; Shoemaker, Sharon P; Mills, David A

    2010-11-01

    Cost effective use of lignocellulosic biomass for bio-based chemical production requires the discovery of novel strains and processes. Lactobacillus pentosus JH5XP5 is a carbon catabolite repression negative mutant which utilizes glucose and pentoses derived from lignocellulosic biomass in the media simultaneously. With a broad range of carbon substrates, L. pentosus JH5XP5 produced a significant amount of ethanol without acetate formation. The yields of ethanol were 2.0- to 2.5-fold higher than those of lactate when glucose, galactose or maltose was used either as a single carbon source or simultaneously with glucose. L. pentosus JH5XP5 was successfully used in an integrated process of simultaneous saccharification and mixed sugar fermentation of rice straw hydrolysate. During the fermentation, the enzyme activities for the saccharification of cellulose were not diminished. Moreover glucose, xylose, and arabinose sugars derived from rice straw hyrolysate were consumed concurrently as if a single carbon source existed and no sugars or cellulosic fiber remained after the fermentation.

  6. Conifer Diterpene Resin Acids Disrupt Juvenile Hormone-Mediated Endocrine Regulation in the Indian Meal Moth Plodia interpunctella.

    PubMed

    Oh, Hyun-Woo; Yun, Chan-Seok; Jeon, Jun Hyoung; Kim, Ji-Ae; Park, Doo-Sang; Ryu, Hyung Won; Oh, Sei-Ryang; Song, Hyuk-Hwan; Shin, Yunhee; Jung, Chan Sik; Shin, Sang Woon

    2017-07-01

    Diterpene resin acids (DRAs) are important components of oleoresin and greatly contribute to the defense strategies of conifers against herbivorous insects. In the present study, we determined that DRAs function as insect juvenile hormone (JH) antagonists that interfere with the juvenile hormone-mediated binding of the JH receptor Methoprene-tolerant (Met) and steroid receptor coactivator (SRC). Using a yeast two-hybrid system transformed with Met and SRC from the Indian meal moth Plodia interpunctella, we tested the interfering activity of 3704 plant extracts against JH III-mediated Met-SRC binding. Plant extracts from conifers, especially members of the Pinaceae, exhibited strong interfering activity, and four active interfering DRAs (7α-dehydroabietic acid, 7-oxodehydroabietic acid, dehydroabietic acid, and sandaracopimaric acid) were isolated from roots of the Japanese pine Pinus densiflora. The four isolated DRAs, along with abietic acid, disrupted the juvenile hormone-mediated binding of P. interpunctella Met and SRC, although only 7-oxodehydroabietic acid disrupted larval development. These results demonstrate that DRAs may play a defensive role against herbivorous insects via insect endocrine-disrupting activity.

  7. The role of juvenile hormone in immune function and pheromone production trade-offs: a test of the immunocompetence handicap principle.

    PubMed Central

    Rantala, Markus J; Vainikka, Anssi; Kortet, Raine

    2003-01-01

    The immunocompetence handicap hypothesis postulates that secondary sexual traits are honest signals of mate quality because the hormones (e.g. testosterone) needed to develop secondary sexual traits have immunosuppressive effects. The best support for predictions arising from the immunocompetence handicap hypothesis so far comes from studies of insects, although they lack male-specific hormones such as testosterone. In our previous studies, we found that female mealworm beetles prefer pheromones of immunocompetent males. Here, we tested how juvenile hormone (JH) affects male investment in secondary sexual characteristics and immune functions in the mealworm beetle, Tenebrio molitor. We injected male mealworm beetles with JH (type III) and found that injection increased the attractiveness of male pheromones but simultaneously suppressed immune functions (phenoloxidase activity and encapsulation). Our results suggest that JH, which is involved in the control of reproduction and morphogenesis, also plays a central role in the regulation of a trade-off between the immune system and sexual advertisement in insects. Thus, the results reflect a general mechanism by which the immunocompetence handicap hypothesis may work in insects. PMID:14613612

  8. RNAi-mediated silencing of vitellogenin gene function turns honeybee ( Apis mellifera) workers into extremely precocious foragers

    NASA Astrophysics Data System (ADS)

    Marco Antonio, David Santos; Guidugli-Lazzarini, Karina Rosa; Do Nascimento, Adriana Mendes; Simões, Zilá Luz Paulino; Hartfelder, Klaus

    2008-10-01

    The switch from within-hive activities to foraging behavior is a major transition in the life cycle of a honeybee ( Apis mellifera) worker. A prominent regulatory role in this switch has long been attributed to juvenile hormone (JH), but recent evidence also points to the yolk precursor protein vitellogenin as a major player in behavioral development. In the present study, we injected vitellogenin double-stranded RNA (dsVg) into newly emerged worker bees of Africanized genetic origin and introduced them together with controls into observation hives to record flight behavior. RNA interference-mediated silencing of vitellogenin gene function shifted the onset of long-duration flights (>10 min) to earlier in life (by 3 4 days) when compared with sham and untreated control bees. In fact, dsVg bees were observed conducting such flights extremely precociously, when only 3 days old. Short-duration flights (<10 min), which bees usually perform for orientation and cleaning, were not affected. Additionally, we found that the JH titer in dsVg bees collected after 7 days was not significantly different from the controls. The finding that depletion of the vitellogenin titer can drive young bees to become extremely precocious foragers could imply that vitellogenin is the primary switch signal. At this young age, downregulation of vitellogenin gene activity apparently had little effect on the JH titer. As this unexpected finding stands in contrast with previous results on the vitellogenin/JH interaction at a later age, when bees normally become foragers, we propose a three-step sequence in the constellation of physiological parameters underlying behavioral development.

  9. Dynamics of dissolved organic matter during four storm events in two forest streams: source, export, and implications for harmful disinfection byproduct formation.

    PubMed

    Yang, Liyang; Hur, Jin; Lee, Sonmin; Chang, Soon-Woong; Shin, Hyun-Sang

    2015-06-01

    Dynamics of river dissolved organic matter (DOM) during storm events have profound influences on the downstream aquatic ecosystem and drinking water safety. This study investigated temporal variations in DOM during four storm events in two forest headwater streams (the EH and JH brooks, South Korea) and the impacts on the disinfection byproducts (DBPs) formation potential. The within-event variations of most DOM quantity parameters were similar to the flow rate in the EH but not in the larger JH brook. The dissolved organic carbon (DOC) showed clockwise and counterclockwise hysteresis with the flow rate in the EH and JH brooks, respectively, indicating the importance of both flow path and DOM source pool size in determining the effects of storm events. The stream DOM became less aromatic/humified from the first to the last event in both brooks, probably due to the increasing fresh plant pool and the decreasing leaf litter pool during the course of rainy season. The DOC export during each event increased 1.3-2.7- and 1.1-7.0-fold by stormflows in the EH and JH brooks, respectively. The leaf litter and soil together was the major DOM source, particularly during early events. The enhanced DOM export probably increases the risks of DBPs formation in disinfection, as indicated by a strong correlation observed between DOC and trihalomethanes formation potential (THMFP). High correlations between two humic-like fluorescent components and THMFP further suggested the potential of assessing THMFP with in situ fluorescence sensors during storms.

  10. Reactive Strength Index: A Poor Indicator of Reactive Strength?

    PubMed

    Healy, Robin; Kenny, Ian; Harrison, Drew

    2017-11-28

    The primary aim was to assess the relationships between reactive strength measures and associated kinematic and kinetic performance variables achieved during drop jumps. A secondary aim was to highlight issues with the use of reactive strength measures as performance indicators. Twenty eight national and international level sprinters, consisting of fourteen men and women, participated in this cross-sectional analysis. Athletes performed drop jumps from a 0.3 m box onto a force platform with dependent variables contact time (CT), landing time (TLand), push-off time (TPush), flight time (FT), jump height (JH), reactive strength index (RSI, calculated as JH / CT), reactive strength ratio (RSR, calculated as FT / CT) and vertical leg spring stiffness (Kvert) recorded. Pearson's correlation test found very high to near perfect relationships between RSI and RSR (r = 0.91 to 0.97), with mixed relationships found between RSI, RSR and the key performance variables, (Men: r = -0.86 to -0.71 between RSI/RSR and CT, r = 0.80 to 0.92 between RSI/RSR and JH; Women: r = -0.85 to -0.56 between RSR and CT, r = 0.71 between RSI and JH). This study demonstrates that the method of assessing reactive strength (RSI versus RSR) may be influenced by the performance strategies adopted i.e. whether an athlete achieves their best reactive strength scores via low CTs, high JHs or a combination. Coaches are advised to limit the variability in performance strategies by implementing upper and / or lower CT thresholds to accurately compare performances between individuals.

  11. Comparative Genomics of Klebsiella pneumoniae Strains with Different Antibiotic Resistance Profiles▿†

    PubMed Central

    Kumar, Vinod; Sun, Peng; Vamathevan, Jessica; Li, Yong; Ingraham, Karen; Palmer, Leslie; Huang, Jianzhong; Brown, James R.

    2011-01-01

    There is a global emergence of multidrug-resistant (MDR) strains of Klebsiella pneumoniae, a Gram-negative enteric bacterium that causes nosocomial and urinary tract infections. While the epidemiology of K. pneumoniae strains and occurrences of specific antibiotic resistance genes, such as plasmid-borne extended-spectrum β-lactamases (ESBLs), have been extensively studied, only four complete genomes of K. pneumoniae are available. To better understand the multidrug resistance factors in K. pneumoniae, we determined by pyrosequencing the nearly complete genome DNA sequences of two strains with disparate antibiotic resistance profiles, broadly drug-susceptible strain JH1 and strain 1162281, which is resistant to multiple clinically used antibiotics, including extended-spectrum β-lactams, fluoroquinolones, aminoglycosides, trimethoprim, and sulfamethoxazoles. Comparative genomic analysis of JH1, 1162281, and other published K. pneumoniae genomes revealed a core set of 3,631 conserved orthologous proteins, which were used for reconstruction of whole-genome phylogenetic trees. The close evolutionary relationship between JH1 and 1162281 relative to other K. pneumoniae strains suggests that a large component of the genetic and phenotypic diversity of clinical isolates is due to horizontal gene transfer. Using curated lists of over 400 antibiotic resistance genes, we identified all of the elements that differentiated the antibiotic profile of MDR strain 1162281 from that of susceptible strain JH1, such as the presence of additional efflux pumps, ESBLs, and multiple mechanisms of fluoroquinolone resistance. Our study adds new and significant DNA sequence data on K. pneumoniae strains and demonstrates the value of whole-genome sequencing in characterizing multidrug resistance in clinical isolates. PMID:21746949

  12. A Generalization of Snell’s Law

    DTIC Science & Technology

    1990-06-01

    Weinstock [Ref. 2:pp. 20-22]. We know that f(x+h) - f(x) = f’(x)h + higher order terms in h. We apply this to our simplest variational problem by considering...higher order terms . We also define 6J[h] to be the first variation : 8J[h] =f’b (Fy h + Fyh ’)& Setting the first variation equal to zero, and integrating...tC.’~.’’ 82 16. SUPPLEMENTARY NOTATION o r-’ ~ A I~ ~rt~i ra -Ir C’t t.t ) o 17, COSATI CODES 18, SyBJECT TERMS (Continue on reverse if necessary

  13. Effects of forage level in feedlot finishing diets on carcass characteristics and palatability of Jersey beef.

    PubMed

    Arnett, E J; Fluharty, F L; Loerch, S C; Zerby, H N; Zinn, R A; Kuber, P S

    2012-03-01

    Jersey cattle are known for producing carcasses with a greater amount of marbling, but they require more days on feed to achieve acceptable market weights compared with other breeds. The objective of this study was to evaluate the effect of dietary forage (12 vs. 24% sudangrass:alfalfa hay, DM basis) in steam-flaked, corn-based finishing diets on carcass characteristics, beef palatability, and retail color stability of steaks from Jersey beef compared with conventionally fed commodity beef strip loins (COM) of identified quality (Choice(-) and Select(+)). Jersey steers (n = 77) were blocked by BW and randomly assigned to 1 of the following treatments for a 383-d trial period: Jersey low 12% (JL; n = 38) or Jersey high 24% (JH; n = 39) forage (DM basis). A comparison group was selected from conventionally fed cattle on the same day of slaughter as the Jersey treatments, and strip loins from USDA Select(+) (COM; n = 20) and Choice(-) (COM; n = 20) were removed for data analysis. Seventy-two hours postmortem, strip loins were removed, vacuum-packaged, and aged at 3°C for 18 d postmortem. After the aging period, steaks from the LM were sliced, vacuum-packaged, and frozen (-20°C) until analyzed. Jersey steaks had reduced (P < 0.05) Warner-Bratzler shear force values compared with COM steaks. Trained sensory panelists rated JL greater (P < 0.05) for initial and sustained tenderness and initial juiciness than COM, whereas JH was intermediate. As expected, marbling was greater (P < 0.05) for both JL and JH compared with COM, and trained sensory panel sustained juiciness, beef flavor intensity, and overall acceptability scores were greater (P < 0.05) for both JL and JH compared with COM; however, no differences (P = 0.14) were reported for consumer tenderness and flavor. Objective color (L*, a*, b*) measurements decreased (P < 0.05) over time across treatments. There were no differences among treatments for lightness (L*); however, overall during retail display JL were

  14. Direct means of obtaining CAVI0-a corrected cardio-ankle vascular stiffness index (CAVI)-from conventional CAVI measurements or their underlying variables.

    PubMed

    Spronck, Bart; Mestanik, Michal; Tonhajzerova, Ingrid; Jurko, Alexander; Jurko, Tomas; Avolio, Alberto P; Butlin, Mark

    2017-09-21

    Cardio-ankle vascular index (CAVI) as measured using the VaSera device (CAVI VS , Fukuda Denshi), has been proposed as a stiffness index that does not depend on blood pressure. We have recently shown theoretically that CAVI VS still exhibits blood pressure dependence, and proposed the corrected index CAVI 0 . The present study aims to establish a method of calculating [Formula: see text] either (i) from VaSera-reported values of cardiac-brachial and brachial-ankle pulse transit times (tb and tba, respectively) and blood pressure, or (ii) directly from CAVI VS . To derive this method, the relationship among CAVI VS and its scale constants a and b, tb, tba, and blood pressure has to be established. From data of 497 subjects, eight candidate CAVI parameters were defined and calculated, containing all combinations of left or right tb/tba/blood pressure. Candidates were evaluated through correlation with measured left and right CAVI VS . Correlations were compared statistically. Once the correct candidates were determined, two constants (a and b) required for converting CAVI VS to CAVI 0 were estimated through linear regression. Left and right CAVI VS are calculated using left and right tba; however, both left and right CAVI VS are calculated using right brachial blood pressures and right tb. Constants a and b for conversion of CAVI VS to CAVI 0 were estimated to be 0.842 [0.836 0.848] and 0.753 [0.721 0.786] (mean [95%CI]), respectively. Equations to estimate CAVI 0 from CAVI VS , and to directly calculate CAVI 0 from the VaSera output are provided in this paper, as well as in a directly usable spreadsheet supplement. Our results permit straightforward calculation of [Formula: see text] during a study, as well as retrospective estimation of [Formula: see text] from CAVI VS in already published studies or where the original transit time values are not available, paving the way for thorough comparison of CAVI 0 to CAVI VS in clinical and research settings. Novelty and

  15. Is POEM the Answer for Management of Spastic Esophageal Disorders? A Systematic Review and Meta-Analysis.

    PubMed

    Khan, Muhammad Ali; Kumbhari, Vivek; Ngamruengphong, Saowanee; Ismail, Amr; Chen, Yen-I; Chavez, Yamile Haito; Bukhari, Majidah; Nollan, Richard; Ismail, Mohammad Kashif; Onimaru, Manabu; Balassone, Valerio; Sharata, Ahmed; Swanstrom, Lee; Inoue, Haruhiro; Repici, Alessandro; Khashab, Mouen A

    2017-01-01

    Spastic esophageal disorders (SEDs) include spastic achalasia (type III), diffuse esophageal spasm (DES), and nutcracker/jackhammer esophagus (JH). Per-oral endoscopic myotomy (POEM) has demonstrated efficacy and safety in the treatment of achalasia. Recently, POEM has been indicated for the treatment of SEDs. We conducted a systematic review and meta-analysis to determine the clinical success and safety of POEM in SEDs. We searched several databases from 01/01/2007 to 01/10/2016 to identify studies (with five or more patients) on POEM for the treatment of SEDs. Weighted pooled rates (WPRs) for clinical success and adverse events (AEs) were calculated for all SEDs. Clinical success was defined as Eckardt scores of ≤3 and/or improvement in severity of dysphagia based on achalasia disease-specific health-related quality of life questionnaire. The WPRs for clinical success and AEs were analyzed using fixed- or random-effects model based on heterogeneity. The proportionate difference in clinical success and post-procedure adverse event rates among individual types of SEDs was also calculated. A total of eight observational studies with 179 patients were included in the final analysis. Two studies were of good quality and six were of fair quality based on the National Institutes of Health quality assessment tool. The WPR with 95% confidence interval (CI) for cumulative clinical success of POEM in all SEDs was 87% (78, 93%), I 2  = 37%. The total number of patients for individual disorders, i.e., type III achalasia, JH, and DES, was 116, 37, and 18, respectively. The WPRs for clinical success of POEM for type III achalasia, DES, and JH were 92, 88, and 72%, respectively. Proportion difference of WPR for clinical success was significantly higher for type III achalasia in comparison with JH (20%, P = 0.01). The WPR with 95% CI for AEs of POEM in all SEDs was 14% (9, 20%), I 2  = 0%. The WPRs for post-procedure adverse events for type III achalasia, DES, and JH were

  16. Validity and Reliability of the 30-s Continuous Jump for Anaerobic Power and Capacity Assessment in Combat Sport

    PubMed Central

    Čular, Drazen; Ivančev, Vladimir; Zagatto, Alessandro M.; Milić, Mirjana; Beslija, Tea; Sellami, Maha; Padulo, Johnny

    2018-01-01

    Cycling test such Wingate anaerobic test (WAnT) is used to measure anaerobic power (AP), but not anaerobic capacity (AC, i.e., the metabolic energy demand). However, in sports that do not involve cycling movements (Karate), the continuous jump for 30 s (vertical jumps for 30 s) has been extensively used to measure anaerobic performance in all young athletes. Limited information’s are available concerning its validity and reliability especially in children. As such, the current study aimed to test validity and reliability of a continuous jumps test (the CJ30s), using WAnT as a reference. Thirteen female Karate kids (age: 11.07 ± 1.32 years; mass: 41.76 ± 15.32 kg; height: 152 ± 11.52 cm; training experience: 4.38 ± 2.14 years) were tested on three separate sessions. The first and second sessions were used to assess the reliability using Intra-class correlation coefficient (ICC) of CJ30s, whereas on the third session WAnT was administered. Following CJ30s and WAnT, we assessed AP (1/CJ30s, as jump height [JH], fatigue index [FI], and blood lactate [BL]; 2/WAnT, as mechanical power [P], FI, and BL) and AC as the excess post-exercise oxygen consumption (EPOC). Large/highly significant correlations were found between CJ30s and WAnT EPOCs (r = 0.730, P = 0.003), and BLs (r = 0.713, P = 0.009). Moderate/significant correlations were found between CJ30s and WAnT FIs (r = 0.640, P = 0.014), CJ30s first four jumps mean JH and WAnT peak P (r = 0.572, P = 0.032), and CJ30s mean JH and WAnT mean P (r = 0.589, P = 0.021). CJ30s showed excellent and moderate reliability (ICC) for AP (maximal JH 0.884, mean JH 0.742, FI 0.657, BL 0.653) and AC (EPOC 0.788), respectively. Correlations observed especially in terms of AC between CJ30s and WAnT provide evidence that former may adequately assess anaerobic performance for the young combat athlete. CJ30 is a reliable test and allow an easy assessment of AP and AC in karate children. PMID:29867580

  17. pHTβ-promoted mobilization of non-conjugative resistance plasmids from Enterococcus faecium to Enterococcus faecalis.

    PubMed

    Di Sante, Laura; Morroni, Gianluca; Brenciani, Andrea; Vignaroli, Carla; Antonelli, Alberto; D'Andrea, Marco Maria; Di Cesare, Andrea; Giovanetti, Eleonora; Varaldo, Pietro E; Rossolini, Gian Maria; Biavasco, Francesca

    2017-09-01

    To analyse the recombination events associated with conjugal mobilization of two multiresistance plasmids, pRUM17i48 and pLAG (formerly named pDO1-like), from Enterococcus faecium 17i48 to Enterococcus faecalis JH2-2. The plasmids from two E. faecalis transconjugants (JH-4T, tetracycline resistant, and JH-8E, erythromycin resistant) and from the E. faecium donor (also carrying a pHTβ-like conjugative plasmid, named pHTβ17i48) were investigated by several methods, including PCR mapping and sequencing, S1-PFGE followed by Southern blotting and hybridization, and WGS. Two locations of repApHTβ were detected in both transconjugants, one on a ∼50 kb plasmid (as in the donor) and the other on plasmids of larger sizes. In JH-4T, WGS disclosed an 88.6 kb plasmid resulting from the recombination of pHTβ17i48 (∼50 kb) and a new plasmid, named pLAG (35.3 kb), carrying the tet(M), tet(L), lsa(E), lnu(B), spw and aadE resistance genes. In JH-8E, a 75 kb plasmid resulting from the recombination of pHTβ17i48 and pRUM17i48 was observed. In both cases, the cointegrates were apparently derived from replicative transposition of an IS1216 present in each of the multiresistance plasmids into pHTβ17i48. The cointegrates could resolve to yield the multiresistance plasmids and a pHTβ17i48 derivative carrying an IS1216 (unlike the pHTβ17i48 of the donor). Our results completed the characterization of the multiresistance plasmids carried by the E. faecium 17i48, confirming the role of pHT plasmids in the mobilization of non-conjugative antibiotic resistance elements among enterococci. Results also revealed that mobilization to E. faecalis was associated with the generation of cointegrate plasmids promoted by IS1216-mediated transposition. © The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Relationship between relative net vertical impulse and jump height in jump squats performed to various squat depths and with various loads.

    PubMed

    McBride, Jeffrey M; Kirby, Tyler J; Haines, Tracie L; Skinner, Jared

    2010-12-01

    The purpose of the current investigation was to determine the relationship between relative net vertical impulse (net vertical impulse (VI)) and jump height in the jump squat (JS) going to different squat depths and utilizing various loads. Ten males with two years of jumping experience participated in this investigation (Age: 21.8 ± 1.9 y; Height: 176.9 ± 5.2 cm; Body Mass: 79.0 ± 7.1 kg, 1RM: 131.8 ± 29.5 kg, 1RM/BM: 1.66 ± 0.27). Subjects performed a series of static jumps (SJS) and countermovement jumps (CMJJS) with various loads (Body Mass, 20% of 1RM, 40% of 1RM) in a randomized fashion to a depth of 0.15, 0.30, 0.45, 0.60, and 0.75 m and a self-selected depth. During the concentric phase of each JS, peak force (PF), peak power (PP), jump height (JH) and relative VI were recorded and analyzed. Increasing squat depth corresponded to a decrease in PF and an increase in JH, relative VI for both SJS and CMJJS during all loads. Across all squat depths and loading conditions relative VI was statistically significantly correlated to JH in the SJS (r = .8956, P < .0001, power = 1.000) and CMJJS (r = .6007, P < .0001, power = 1.000). Across all squat depths and loading conditions PF was statistically nonsignificantly correlated to JH in the SJS (r = -0.1010, P = .2095, power = 0.2401) and CMJJS (r = -0.0594, P = .4527, power = 0.1131). Across all squat depths and loading conditions peak power (PP) was significantly correlated with JH during both the SJS (r = .6605, P < .0001, power = 1.000) and the CMJJS (r = .6631, P < .0001, power = 1.000). PP was statistically significantly higher at BM in comparison with 20% of 1RM and 40% of 1RM in the SJS and CMJJS across all squat depths. Results indicate that relative VI and PP can be used to predict JS performance, regardless of squat depth and loading condition. However, relative VI may be the best predictor of JS performance with PF being the worst predictor of JS performance.

  19. Returners Exhibit Greater Jumping Performance Improvements During a Peaking Phase Compared With New Players on a Volleyball Team.

    PubMed

    Bazyler, Caleb D; Mizuguchi, Satoshi; Kavanaugh, Ashley A; McMahon, John J; Comfort, Paul; Stone, Michael H

    2018-06-21

    To determine if jumping-performance changes during a peaking phase differed among returners and new players on a female collegiate volleyball team and to determine which variables best explained the variation in performance changes. Fourteen volleyball players were divided into 2 groups-returners (n = 7) and new players (n = 7)-who completed a 5-wk peaking phase prior to conference championships. Players were tested at baseline before the preseason on measures of the vastus lateralis cross-sectional area using ultrasonography, estimated back-squat 1-repetition maximum, countermovement jump height (JH), and relative peak power on a force platform. Jumping performance, rating of perceived exertion training load, and sets played were recorded weekly during the peaking phase. There were moderate to very large (P < .01, Glass Δ = 1.74) and trivial to very large (P = .07, Δ = 1.09) differences in JH and relative peak power changes in favor of returners over new players, respectively, during the peaking phase. Irrespective of group, 7 of 14 players achieved peak JH 2 wk after the initial overreach. The number of sets played (r = .78, P < .01) and the athlete's preseason relative 1-repetition maximum (r = .54, P = .05) were the strongest correlates of JH changes during the peaking phase. Returners achieved greater improvements in jumping performance during the peaking phase compared with new players, which may be explained by the returners' greater relative maximal strength, time spent competing, and training experience. Thus, volleyball and strength coaches should consider these factors when prescribing training during a peaking phase to ensure their players are prepared for important competitions.

  20. Biopharmaceutical characterization of decursin and their derivatives for drug discovery.

    PubMed

    Mahat, Bimit; Chae, Jung-woo; Baek, In-hwan; Song, Gyu-yong; Song, Jin-sook; Ma, Jin-yeul; Kwon, Kwang-il

    2013-10-01

    Angelica gigas Nakai and its components are known to have neuroprotective, antiplatelet, and anticancer activities. The present study evaluated the in vitro and in vivo biopharmaceutical characterization of Angelica gigas component substances, including decursin (the main substance), decursinol angelate (decursin isomer), JH714 (ether form of decursin) and epoxide decursin (epoxide form of decursin). Decursin, decursinol angelate and JH714 exhibited acceptable metabolic stability (>50%) in liver microsomes from human and higher bound fraction (>90%) in human plasma operating ultrafiltration. Decursin and decursinol angelate in CYP1A2 and CYP2C19 indicated less than 50% CYP activity, suggesting inhibition of the CYP isoforms using Vivid® CYP screening kit. JH714 only showed an apparent permeability coefficient of <10 × 10⁻⁶ cm/s in MDCK cells, suggesting that it is poorly absorbed. Blood brain barrier permeability was examined after oral administration to male Sprague-Dawley (SD) rats, and pharmacokinetic studies were performed after oral and intravenous administration of 10 mg/kg compounds. Decursin, decursinol angelate and JH714 showed ratios of compound concentration in brain with respect to plasma (Cbrain/Cplasma) of >1.5, suggesting good brain/plasma ratio at 0.5, 1, 3, and 5 h. In contrast, Cbrain/Cplasma was <0.5 for epoxide decursin. For all test compounds, >1.5% of the dose remained in GI tract after 8 h, and the excretion rate in urine was <0.5% which suggests that gastro intestinal tract may be major site of disposition following oral administration. Finally, these results may be useful for the design of dosage regimens of decursin and its derivatives.

  1. A prospective cohort study investigating the effect of generalized joint hypermobility on outcomes after arthroscopic anterior shoulder stabilization.

    PubMed

    Koyonos, Loukas; Kraeutler, Matthew J; O'Brien, Daniel F; Ciccotti, Michael G; Dodson, Christopher C

    2016-11-01

    Generalized joint laxity has been proposed as a significant risk factor for failure after arthroscopic anterior shoulder stabilization. The purpose of this study was to prospectively measure joint mobility in patients undergoing arthroscopic anterior shoulder stabilization and to determine whether hypermobility is a risk factor for worse outcomes compared with patients having normal joint mobility. Patients with anterior shoulder instability were prospectively enrolled. Generalized joint hypermobility was measured using the Beighton Hypermobility Score and the Rowe, UCLA, SANE, SST, and WOSI scores were administered and reported as patient outcomes preoperatively and following arthroscopic anterior shoulder stabilization at 6 weeks, 6 months, 12 months, and 24 months postoperatively. Patients were stratified into two groups based on their Beighton Hypermobility Score, with scores ≥ 4/9 indicative of joint hypermobility. Sixteen patients with joint hypermobility (JH) and 18 non-hypermobile patients (NJH) were enrolled. At baseline, there were no significant differences in demographic characteristics or baseline patient-reported outcomes. Significantly more patients in the NJH group had SLAP tears (n = 10) compared to the JH group (n = 2) (p = .013). At all follow-up times, there were no significant differences between the NJH and JH groups with regard to patient-reported outcome scores (p > .05). In the JH group, 17% of patients reported recurrent instability at two years postoperatively compared to 25% of patients in the NJH group. There was no significant difference in failure rate (p = .67). There was no significant difference in patient-reported outcomes or recurrent instability in patients with versus without joint hypermobility undergoing arthroscopic anterior shoulder stabilization.

  2. The effects of juvenile hormone on Lasius niger reproduction.

    PubMed

    Pamminger, T; Buttstedt, A; Norman, V; Schierhorn, A; Botías, C; Jones, J C; Basley, K; Hughes, W O H

    2016-12-01

    Reproduction has been shown to be costly for survival in a wide diversity of taxa. The resulting trade-off, termed the reproduction-survival trade-off, is thought to be one of the most fundamental forces of life-history evolution. In insects the pleiotropic effect of juvenile hormone (JH), antagonistically regulating reproduction and pathogen resistance, is suggested to underlie this phenomenon. In contrast to the majority of insects, reproductive individuals in many eusocial insects defy this trade-off and live both long and prosper. By remodelling the gonadotropic effects of JH in reproductive regulation, the queens of the long-lived black garden ant Lasius niger (living up to 27 years), have circumvented the reproduction-survival trade off enabling them to maximize both reproduction and pathogen resistance simultaneously. In this study we measure fertility, vitellogenin gene expression and protein levels after experimental manipulation of hormone levels. We use these measurements to investigate the mechanistic basis of endocrinological role remodelling in reproduction and determine how JH suppresses reproduction in this species, rather then stimulating it, like in the majority of insects. We find that JH likely inhibits three key aspects of reproduction both during vitellogenesis and oogenesis, including two previously unknown mechanisms. In addition, we document that juvenile hormone, as in the majority of insects, has retained some stimulatory function in regulating vitellogenin expression. We discuss the evolutionary consequences of this complex regulatory architecture of reproduction in L. niger, which might enable the evolution of similar reproductive phenotypes by alternate regulatory pathways, and the surprising flexibility regulatory role of juvenile hormone in this process. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Improving the Signal-To-Noise Ratio When Monitoring Countermovement Jump Performance.

    PubMed

    Kennedy, Rodney A; Drake, David

    2018-05-08

    Kennedy, RA and Drake, D. Improving the signal-to-noise ratio when monitoring countermovement jump performance. J Strength Cond Res XX(X): 000-000, 2018-Countermovement jump (CMJ) performance has been routinely used to monitor neuromuscular status. However, the protocol used to establish the criterion score is not well documented. The purpose of this study was to examine how the protocol used would influence of the sensitivity of CMJ variables in rugby union players. Fifteen male (age: 19.7 ± 0.5 years) rugby union players performed 8 CMJs on 2 occasions, separated by 7 days. The between-session coefficient of variation (CV) was calculated using 2 techniques for treating multiple trials, the average, and the trial with the best jump height (JH), and then compared with the smallest worthwhile change (SWC). The signal-to-noise ratio was measured as the group mean change in a variable divided by the CV. Using the average value across multiple trials is superior to the best trial method, based on lower CVs for all variables. Only the average performance across 6 or more trials was classified as ideal (CV < 0.5 × SWC) for peak velocity (PV). In addition, the signal-to-noise ratio for peak concentric power (PCP), PV, and JH were classified as good, irrespective of the treatment method. Although increasing the number of trials can reduce the random error, it may be pragmatic to simply take the average from 2 to 3 trials, facilitating a CV < SWC for PV, PCP, and JH. Due to its simplicity, JH may be considered the principal variable to monitor neuromuscular fatigue.

  4. Using Value Stream Mapping to improve quality of care in low-resource facility settings.

    PubMed

    Ramaswamy, Rohit; Rothschild, Claire; Alabi, Funmi; Wachira, Eric; Muigai, Faith; Pearson, Nick

    2017-11-01

    Jacaranda Health (JH) is a Kenya-based organization that attempts to provide affordable, high-quality maternal and newborn healthcare through a chain of private health facilities in Nairobi. JH needed to adopted quality improvement as an organization-wide strategy to optimize effectiveness and efficiency. Value Stream Mapping, a Lean Management tool, was used to engage staff in prioritizing opportunities to improve clinical outcomes and patient-centered quality of care. Implementation was accomplished through a five-step process: (i) leadership engagement and commitment; (ii) staff training; (iii) team formation; (iv) process walkthrough; and (v) construction and validation. The Value Stream Map allowed the organization to come together and develop an end-to-end view of the process of care at JH and to select improvement opportunities for the entire system. The Value Stream Map is a simple visual tool that allows organizations to engage staff at all levels to gain commitment around quality improvement efforts. © The Author 2017. Published by Oxford University Press in association with the International Society for Quality in Health Care. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

  5. Attention-deficit/hyperactivity disorder, joint hypermobility-related disorders and pain: expanding body-mind connections to the developmental age.

    PubMed

    Baeza-Velasco, Carolina; Sinibaldi, Lorenzo; Castori, Marco

    2018-02-14

    Attention-deficit/hyperactivity disorder (ADHD) and generalized joint hypermobility (JH) are two separated conditions, assessed, and managed by different specialists without overlapping interests. Recently, some researchers highlighted an unexpected association between these two clinical entities. This happens in a scenario of increasing awareness on the protean detrimental effects that congenital anomalies of the connective tissue may have on human health and development. To review pertinent literature to identify possible connections between ADHD and GJH, special emphasis was put on musculoskeletal pain and syndromic presentations of GJH, particularly the hypermobile Ehlers-Danlos syndrome. A comprehensive search of scientific databases and references lists was conducted, encompassing publications based on qualitative and quantitative research. Impaired coordination and proprioception, fatigue, chronic pain, and dysautonomia are identified as potential bridges between ADHD and JH. Based on these findings, a map of the pathophysiological and psychopathological pathways connecting both conditions is proposed. Although ADHD and JH are traditionally separated human attributes, their association may testify for the dyadic nature of mind-body connections during critical periods of post-natal development. Such a mixed picture has potentially important consequences in terms of disability and deserves more clinical and research attention.

  6. Hypermetabolic Conversion of Plant Oil into Water: Endothermic Biochemical Process Stimulated by Juvenile Hormone in the European Firebug, Pyrrhocoris apterus L.

    PubMed

    Sláma, Karel; Lukáš, Jan

    2016-01-01

    The physiological and biochemical mechanisms that enable insects to feed on dry food to secure enough water for larval growth were investigated. The study was carried out with a plethora of physiological methods, ranging from the simple volumetric determination of O 2 consumption and water intake to more advanced methods such as scanning microrespirography and thermovision imaging of insect's body temperature. The experiments were done on the European firebug, Pyrrhocoris apterus , which feeds exclusively on dry linden seeds. In order to survive, it needs to drink water or suck a sap from plants occasionally. It was found that the young larval instars compensate the occasional water deficiency by the increased production of metabolic water. The juvenile hormone (JH)-dependent production of metabolic water, which was previously found in other species consuming dry food, was achieved in P. apterus by total metabolic combustion of the dietary lipid (neutral seed oil). The water-producing, hypermetabolic larvae were heated from inside by endothermic energy released from the uncoupling of oxidation from oxidative phosphorylation. The "warm", hypermetabolic larvae burning the dietary oil into CO 2 and water showed the increased rates of respiratory metabolism. Microrespirographic recording of these larvae revealed the ratio of the respiratory quotient (RQ, CO 2 /O 2 ) of 0.7, which indicated the breakdown of a pure triglyceride. The warm hypermetabolic larvae could be easily spotted and distinguished from the "cold" larvae on the screen of a thermovision camera. The last instar larvae lacking the JH were always only cold. They metabolized a carbohydrate substrate exclusively (RQ = 1.0), while the dietary lipid was stored in the fat body. In comparison with the hypermetabolic larvae of some other species fed on dry food, which exhibited the highest rates of O 2 consumption ever recorded in a living organism (10-20 mL O 2 /g per hour), the metabolic difference between the

  7. Mitochondrial antioxidative capacity regulates muscle glucose uptake in the conscious mouse: effect of exercise and diet.

    PubMed

    Kang, Li; Lustig, Mary E; Bonner, Jeffrey S; Lee-Young, Robert S; Mayes, Wesley H; James, Freyja D; Lin, Chien-Te; Perry, Christopher G R; Anderson, Ethan J; Neufer, P Darrell; Wasserman, David H

    2012-10-15

    The objective of this study was to test the hypothesis that exercise-stimulated muscle glucose uptake (MGU) is augmented by increasing mitochondrial reactive oxygen species (mtROS) scavenging capacity. This hypothesis was tested in genetically altered mice fed chow or a high-fat (HF) diet that accelerates mtROS formation. Mice overexpressing SOD2 (sod2(Tg)), mitochondria-targeted catalase (mcat(Tg)), and combined SOD2 and mCAT (mtAO) were used to increase mtROS scavenging. mtROS was assessed by the H(2)O(2) emitting potential (JH(2)O(2)) in muscle fibers. sod2(Tg) did not decrease JH(2)O(2) in chow-fed mice, but decreased JH(2)O(2) in HF-fed mice. mcat(Tg) and mtAO decreased JH(2)O(2) in both chow- and HF-fed mice. In parallel, the ratio of reduced to oxidized glutathione (GSH/GSSG) was unaltered in sod2(Tg) in chow-fed mice, but was increased in HF-fed sod2(Tg) and both chow- and HF-fed mcat(Tg) and mtAO. Nitrotyrosine, a marker of NO-dependent, reactive nitrogen species (RNS)-induced nitrative stress, was decreased in both chow- and HF-fed sod2(Tg), mcat(Tg), and mtAO mice. This effect was not changed with exercise. Kg, an index of MGU was assessed using 2-[(14)C]-deoxyglucose during exercise. In chow-fed mice, sod2(Tg), mcat(Tg), and mtAO increased exercise Kg compared with wild types. Exercise Kg was also augmented in HF-fed sod2(Tg) and mcat(Tg) mice but unchanged in HF-fed mtAO mice. In conclusion, mtROS scavenging is a key regulator of exercise-mediated MGU and this regulation depends on nutritional state.

  8. Transcriptome reprogramming of resistant and susceptible peach genotypes during Xanthomonas arboricola pv. pruni early leaf infection

    PubMed Central

    Gervasi, Fabio; Ferrante, Patrizia; Dettori, Maria Teresa; Scortichini, Marco

    2018-01-01

    Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is a major threat to Prunus species worldwide. The molecular mechanisms of peach resistance to Xap during early leaf infection were investigated by RNA-Seq analysis of two Prunus persica cultivars, ‘Redkist’ (resistant), and ‘JH Hale’ (susceptible) at 30 minutes, 1 and 3 hours-post-infection (hpi). Both cultivars exhibited extensive modulation of gene expression at 30 mpi, which reduced significantly at 1 hpi, increasing again at 3 hpi. Overall, 714 differentially expressed genes (DEGs) were detected in ‘Redkist’ (12% at 30 mpi and 1 hpi and 88% at 3 hpi). In ‘JH Hale’, 821 DEGs were identified (47% at 30 mpi and 1 hpi and 53% at 3 hpi). Highly up-regulated genes (fold change > 100) at 3 hpi exhibited higher fold change values in ‘Redkist’ than in ‘JH Hale’. RNA-Seq bioinformatics analyses were validated by RT-qPCR. In both cultivars, DEGs included genes with putative roles in perception, signal transduction, secondary metabolism, and transcription regulation, and there were defense responses in both cultivars, with enrichment for the gene ontology terms, ‘immune system process’, ‘defense response’, and ‘cell death’. There were particular differences between the cultivars in the intensity and kinetics of modulation of expression of genes with putative roles in transcriptional activity, secondary metabolism, photosynthesis, and receptor and signaling processes. Analysis of differential exon usage (DEU) revealed that both cultivars initiated remodeling their transcriptomes at 30 mpi; however, ‘Redkist’ exhibited alternative exon usage for a greater number of genes at every time point compared with ‘JH Hale’. Candidate resistance genes (WRKY-like, CRK-like, Copper amine oxidase-like, and TIR-NBS-LRR-like) are of interest for further functional characterization with the aim of elucidating their role in Prunus spp. resistance to Xap. PMID:29698473

  9. Transcriptome reprogramming of resistant and susceptible peach genotypes during Xanthomonas arboricola pv. pruni early leaf infection.

    PubMed

    Gervasi, Fabio; Ferrante, Patrizia; Dettori, Maria Teresa; Scortichini, Marco; Verde, Ignazio

    2018-01-01

    Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is a major threat to Prunus species worldwide. The molecular mechanisms of peach resistance to Xap during early leaf infection were investigated by RNA-Seq analysis of two Prunus persica cultivars, 'Redkist' (resistant), and 'JH Hale' (susceptible) at 30 minutes, 1 and 3 hours-post-infection (hpi). Both cultivars exhibited extensive modulation of gene expression at 30 mpi, which reduced significantly at 1 hpi, increasing again at 3 hpi. Overall, 714 differentially expressed genes (DEGs) were detected in 'Redkist' (12% at 30 mpi and 1 hpi and 88% at 3 hpi). In 'JH Hale', 821 DEGs were identified (47% at 30 mpi and 1 hpi and 53% at 3 hpi). Highly up-regulated genes (fold change > 100) at 3 hpi exhibited higher fold change values in 'Redkist' than in 'JH Hale'. RNA-Seq bioinformatics analyses were validated by RT-qPCR. In both cultivars, DEGs included genes with putative roles in perception, signal transduction, secondary metabolism, and transcription regulation, and there were defense responses in both cultivars, with enrichment for the gene ontology terms, 'immune system process', 'defense response', and 'cell death'. There were particular differences between the cultivars in the intensity and kinetics of modulation of expression of genes with putative roles in transcriptional activity, secondary metabolism, photosynthesis, and receptor and signaling processes. Analysis of differential exon usage (DEU) revealed that both cultivars initiated remodeling their transcriptomes at 30 mpi; however, 'Redkist' exhibited alternative exon usage for a greater number of genes at every time point compared with 'JH Hale'. Candidate resistance genes (WRKY-like, CRK-like, Copper amine oxidase-like, and TIR-NBS-LRR-like) are of interest for further functional characterization with the aim of elucidating their role in Prunus spp. resistance to Xap.

  10. Ingrown toenail

    MedlinePlus

    ... In: Azar FM, Beaty JH, Canale ST, eds. Campbell's Operative Orthopaedics . 13th ed. Philadelphia, ... by: Thomas N. Joseph, MD, Private Practice specializing in Orthopaedics, Subspecialty Foot ...

  11. Taking care of your new hip joint

    MedlinePlus

    Hip arthroplasty - precautions; Hip replacement - precautions; Osteoarthritis - hip; Osteoarthritis - knee ... Saunders; 2015:chap 61. Harkess JW, Crockarell JR. Arthroplasty of the hip. In: Azar FM, Beaty JH, ...

  12. Physiological and Behavioral Changes in Honey Bees (Apis mellifera) Induced by Nosema ceranae Infection

    PubMed Central

    Goblirsch, Mike; Huang, Zachary Y.; Spivak, Marla

    2013-01-01

    Persistent exposure to mite pests, poor nutrition, pesticides, and pathogens threaten honey bee survival. In healthy colonies, the interaction of the yolk precursor protein, vitellogenin (Vg), and endocrine factor, juvenile hormone (JH), functions as a pacemaker driving the sequence of behaviors that workers perform throughout their lives. Young bees perform nursing duties within the hive and have high Vg and low JH; as older bees transition to foraging, this trend reverses. Pathogens and parasites can alter this regulatory network. For example, infection with the microsporidian, Nosema apis, has been shown to advance behavioral maturation in workers. We investigated the effects of infection with a recent honey bee pathogen on physiological factors underlying the division of labor in workers. Bees infected with N. ceranae were nearly twice as likely to engage in precocious foraging and lived 9 days less, on average, compared to controls. We also show that Vg transcript was low, while JH titer spiked, in infected nurse-aged bees in cages. This pattern of expression is atypical and the reverse of what would be expected for healthy, non-infected bees. Disruption of the basic underpinnings of temporal polyethism due to infection may be a contributing factor to recent high colony mortality, as workers may lose flexibility in their response to colony demands. PMID:23483987

  13. Juvenile hemochromatosis: HAMP mutation and severe iron overload treated with phlebotomies and deferasirox.

    PubMed

    Lescano, Manuel A; Tavares, Letícia C; Santos, Paulo C J L

    2017-10-16

    Juvenile hemochromatosis (JH) is a rare condition classified as an autosomal recessive disorder that leads to severe iron absorption. JH usually affects people under the age of 30 and presents symptoms such as chronic liver damage, hypogonadotropic hypogonadism, cardiac diseases and endocrine dysfunctions. The present case reports a 29-year-old Brazilian woman with JH condition due to HAMP mutation (g.47G>A), treated with phlebotomies and deferasirox. She presented symptoms such as weakness, skin hyperpigmentation, joint pain in the shoulders and hands and amenorrhea. First laboratory tests showed altered biochemical parameters [serum ferritin (SF): 5696 ng/mL, transferrin saturation (TS): 85%]. After sessions of phlebotomies (450 mL every 15 d), the patient presented partial symptomatic improvements and biochemical parameters (SF: 1000 ng/mL, Hb: 11 g/dL). One year later, deferasirox (15 mg/kg per day) was introduced to the treatment, and the patient showed total symptomatic improvement, with significant clearing of the skin, SF: 169 ng/mL, and TS: 50%. Furthermore, after the combined deferasirox-phlebotomy therapy, magnetic resonance imaging measurements revealed normalized level for liver iron (30 μmol/g; reference value < 36 μmol/g). In conclusion, combined deferasirox-phlebotomy treatment was able to normalize iron levels and improve symptoms.

  14. Juvenile hemochromatosis: HAMP mutation and severe iron overload treated with phlebotomies and deferasirox

    PubMed Central

    Lescano, Manuel A; Tavares, Letícia C; Santos, Paulo C J L

    2017-01-01

    Juvenile hemochromatosis (JH) is a rare condition classified as an autosomal recessive disorder that leads to severe iron absorption. JH usually affects people under the age of 30 and presents symptoms such as chronic liver damage, hypogonadotropic hypogonadism, cardiac diseases and endocrine dysfunctions. The present case reports a 29-year-old Brazilian woman with JH condition due to HAMP mutation (g.47G>A), treated with phlebotomies and deferasirox. She presented symptoms such as weakness, skin hyperpigmentation, joint pain in the shoulders and hands and amenorrhea. First laboratory tests showed altered biochemical parameters [serum ferritin (SF): 5696 ng/mL, transferrin saturation (TS): 85%]. After sessions of phlebotomies (450 mL every 15 d), the patient presented partial symptomatic improvements and biochemical parameters (SF: 1000 ng/mL, Hb: 11 g/dL). One year later, deferasirox (15 mg/kg per day) was introduced to the treatment, and the patient showed total symptomatic improvement, with significant clearing of the skin, SF: 169 ng/mL, and TS: 50%. Furthermore, after the combined deferasirox-phlebotomy therapy, magnetic resonance imaging measurements revealed normalized level for liver iron (30 μmol/g; reference value < 36 μmol/g). In conclusion, combined deferasirox-phlebotomy treatment was able to normalize iron levels and improve symptoms. PMID:29085829

  15. Effects of photoperiod and temperature on the rate of larval development, food conversion efficiency, and imaginal diapause in Leptinotarsa decemlineata.

    PubMed

    Dolezal, Petr; Habustová, Oxana; Sehnal, Frantisek

    2007-08-01

    Larvae of Leptinotarsa decemlineata developed faster and consumed less food under short-day (Sd, 12:12 h light:darkness) than under long-day (Ld, 18:6 h L:D) conditions. The average index of food conversion efficiency was 5.4 in the Ld (25 degrees C), and 7.2 and 11.9 (at 20 and 25 degrees C, respectively) in the Sd insects. Pupae were smaller under the Ld conditions due to a greater loss of biomass during the prepupal period that was nearly twice longer than in the Sd insects. Virgin Ld females laid eggs for 6 months and survived 13 months. The lack of oviposition, reduced food intake, and behavioural changes characterised diapause in the Sd adults. Application of 100 microg JH III to newly ecdysed adults was used to probe diapause intensity. At 25 degrees C, the treatment elicited oviposition most effectively in females that were just transferred from the Ld to the Sd conditions. A distinctly lower response occurred in insects that had been kept under Sd conditions since hatching; their transfer to Ld conditions at the time of treatment had little effect on JH sensitivity. JH application to Sd females reared at 20 degrees C caused enlargement of the germaria but no eggs were formed.

  16. A high stability Ni-La0.5Ce0.5O2-δ asymmetrical metal-ceramic membrane for hydrogen separation and generation

    NASA Astrophysics Data System (ADS)

    Zhu, Zhiwen; Sun, Wenping; Wang, Zhongtao; Cao, Jiafeng; Dong, Yingchao; Liu, Wei

    2015-05-01

    In this work, hydrogen permeation properties of Ni-La0.5Ce0.5O2-δ (LDC) asymmetrical cermet membrane are investigated, including hydrogen fluxes (JH2) under different hydrogen partial pressures, the influence of water vapor on JH2 and the long-term stability of the membrane operating under the containing-CO2 atmosphere. Ni-LDC asymmetrical membrane shows the best hydrogen permeability among LDC-based hydrogen separation membranes, inferior to Ni-BaZr0.1Ce0.7Y0.2O3-δ asymmetrical membrane. The water vapor in feed gas is beneficial to hydrogen transport process, which promote an increase of JH2 from 5.64 × 10-8 to 6.83 × 10-8 mol cm-2 s-1 at 900 °C. Stability testing of hydrogen permeation suggests that Ni-LDC membrane remains stable against CO2. A dual function of combining hydrogen separation and generation can be realized by humidifying the sweep gas and enhance the hydrogen output by 1.0-1.5 times. Ni-LDC membrane exhibits desirable performance and durability in dual-function mode. Morphologies and phase structures of the membrane after tests are also characterized by SEM and XRD.

  17. Urban Obsolescence and Its Educational Implications: A Spatial Perspective

    ERIC Educational Resources Information Center

    Emery, J. S.

    1973-01-01

    In a comparative study of six sample London districts the influence of urban obsolescence, socio-economic class, educational provision, and measured intelligence is correlated with pupil achievement. (JH)

  18. Some Effects of Teaching Adolescents: Some Creative, Peaceful Conflict Resolution Strategies

    ERIC Educational Resources Information Center

    Fogg, Richard

    1974-01-01

    Twenty-seven approaches to conflict resolution, representing the creativity of behavioral scientists, are included to indicate the inexhaustible number of means for dealing with conflict without using violence. (JH)

  19. Hormones, metabolites, and reproduction in Holsteins, Jerseys, and their crosses.

    PubMed

    Brown, K L; Cassell, B G; McGilliard, M L; Hanigan, M D; Gwazdauskas, F C

    2012-02-01

    Holsteins (HH), Jerseys (JJ), and their crosses in first (n=157) and second (n=107) lactation were used to determine if reproduction, progesterone (P4), insulin-like growth factor 1 (IGF-1), insulin, nonesterified fatty acids (NEFA), and milk production differed between genetic groups. Thirty-four cows were Holstein-Jersey (HJ) crosses, 46 were Jersey-Holstein (JH) crosses, 48 were purebred Holsteins (HH), and 29 were purebred Jerseys (JJ) in first lactation, whereas the second-lactation animals included 23 HJ, 35 JH, 35 HH, and 14 JJ. Blood samples were collected weekly for the first 10 wk postpartum. Analyses were conducted using the MIXED, chi-square, and GLIMMIX procedures (SAS Institute Inc., Cary, NC). Seasons of calving were cold (November to May) and hot (June to October) and were combined with year to form 8 year-seasons. Days open and number of services were affected by genetic group. The HH were open 169±8 d, which was greater than HJ (143±9 d), JJ (132±10 d), and JH (127±8 d). The HH had 2.4±0.1 services per pregnancy, which was greater than JH (1.9±0.1), but not different from HJ (2.1±0.2) or JJ (2.1±0.2). Concentrations of NEFA were greater in lactation 2 (0.52±0.02 mEq/L) than in lactation 1 (0.45±0.02 mEq/L) and decreased over the 10-wk period. Concentrations of NEFA were greater in the cold season except in yr 3. Insulin in lactation 1 (0.81±0.03 ng/mL) was greater than in lactation 2 (0.72±0.03 ng/mL); insulin decreased to wk 2 then gradually increased. The HJ had the greatest insulin concentrations (0.87±0.04 ng/mL) and the JJ had the lowest (0.66±0.04 ng/mL), and IGF-1 gradually increased over the 10-wk period. Milk production (actual yield in the first 305 d, not adjusted for fat and protein) was affected by genetic group, lactation number, year-season, and wk 1 insulin. The HH produced 10,348±207 kg of milk, which was greater than the HJ (9,129±230 kg), the JH (9,384±190 kg), and the JJ (7,080±240 kg). Milk production in

  20. 76 FR 14682 - Receipt of Applications for Endangered Species Permits

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-17

    ... Tennessee yellow-eyed grass Xyris tennesseensis Etowah Darter Etheostoma etowahae Amber Darter Percina.... Applicant: Dr. J.H. Carter III and Assoc., TE-807672. The applicant requests renewal of authorization for...

  1. Children's Literature in the Soviet Union

    ERIC Educational Resources Information Center

    Miller, D. D.; And Others

    1976-01-01

    Children's literature in the Soviet Union is of four types: 17 stories based on old tales, adaptations from great Russian literature, original writings for children, and translations from foreign works. (JH)

  2. Immunotherapy: questions to ask your doctor

    MedlinePlus

    ... Schroeder H, Frew AJ, Weyand CM, eds. Clinical Immunology . 4th ed. Philadelphia, PA: Elsevier Saunders; 2013:chap 77. Pardoll D. Cancer immunology. In: Niederhuber JE, Armitage JO, Doroshow JH, Kastan ...

  3. Ankle replacement

    MedlinePlus

    ... C, Anderson PA, eds. Skeletal Trauma: Basic Science, Management, and Reconstruction . 5th ed. Philadelphia, PA: Elsevier Saunders; 2015:chap 68. Murphy GA. Total ankle arthroplasty. In: Azar FM, Beaty JH, Canale ...

  4. Effects of soldier-derived terpenes on soldier caste differentiation in the termite Reticulitermes flavipes.

    PubMed

    Tarver, Matthew R; Schmelz, Eric A; Rocca, James R; Scharf, Michael E

    2009-02-01

    Primer pheromones play key roles in regulating division of labor, which is a fundamental and defining aspect of insect sociality. Primer pheromones are chemical messengers that transmit hormone-like messages among colony members; in recipients, these messages can either induce or suppress phenotypic caste differentiation. Here, we investigated soldier caste-derived chemicals as possible primer pheromones in the lower termite Reticulitermes flavipes, a species for which no primer pheromones have yet been identified. We determined that soldier head extracts (SHE), when provided to totipotent workers along with the insect morphogenetic juvenile hormone (JH), significantly enhanced soldier caste differentiation. When applied alone, however, SHE had no impacts on caste differentiation, survivorship, or any other aspect of worker biology. These findings support a function of soldier chemicals as primer pheromones that enhance the action of the endogenous JH. In accord with previous studies, gamma-cadinene and the corresponding aldehyde, gamma-cadinenal, were identified by gas chromatography-mass spectrometry and nuclear magnetic resonance analyses as the two most abundant components of R. flavipes SHE. Validative bioassays with commercially available cadinene confirmed activity. Several other terpenes, previously identified in R. flavipes soldiers, also were found to be active. These findings reveal a novel primer pheromone-like function for soldier-derived terpenes in termites and further suggest convergent evolution of terpene functions in enhancing JH-dependent soldier caste differentiation.

  5. Spatial Representation by Blind and Sighted Children

    ERIC Educational Resources Information Center

    Millar, Susanna

    1976-01-01

    Problem studied: How children represent haptic spatial information in memory. Question aimed at: Whether, and if so in what ways, children's spatial representations differ according to the main modality of prior experience. (JH)

  6. Closed reduction of a fractured bone - aftercare

    MedlinePlus

    ... C, Anderson PA, eds. Skeletal Trauma: Basic Science, Management, and Reconstruction . 5th ed. Philadelphia, PA: Elsevier Saunders; 2015:chap 6. Wood GW. General principles of fracture treatment. In: Canale ST, Beaty JH, ...

  7. Closed reduction of a fractured bone

    MedlinePlus

    ... C, Anderson PA, eds. Skeletal Trauma: Basic Science, Management, and Reconstruction . 5th ed. Philadelphia, PA: Elsevier Saunders; 2015:chap 6. Wood GW. General principles of fracture treatment. In: Canale ST, Beaty JH, ...

  8. The acute effects of back squats on vertical jump performance in men and women.

    PubMed

    Witmer, Chad A; Davis, Shala E; Moir, Gavin L

    2010-01-01

    The aim of the present study was to investigate the acute effects of performing back squats on subsequent performance during a series of vertical jumps in men and women. Twelve men and 12 women were tested on three separate occasions, the first of which was used to determine their 1-repetition maximum (1-RM) parallel back squat. Following this, subjects performed a potentiation and a control treatment in a counterbalanced order. The potentiation treatment culminated with subjects performing parallel back squats with a load equivalent to 70% 1- RM for three repetitions, following which they performed one countermovement vertical jump (CMJ) for maximal height every three minutes for a total of 10 jumps. During the control treatment, subjects performed only the CMJs. Jump height (JH) and vertical stiffness (VStiff) were calculated for each jump from the vertical force signal recorded from a force platform. There were no significant changes in JH or VStiff following the treatments and no significant differences in the responses between men and women (p > 0.05). Correlations between normalized 1-RM back squat load and the absolute change in JH and VStiff were small to moderate for both men and women, with most correlations being negative. Large variations in response to the back squats were noted in both men and women. The use of resistance exercises performed prior to a series of vertical jumps can result in improvements in performance in certain individuals, although the gains tend to be small and dependent upon the mechanical variable measured. There does not seem to be any differences between men and women in the response to dynamic potentiation protocols. Key pointsSubstantial individual responses were noted in both men and women in response to the PAP protocol used in the present study.The choice of dependent variable influences the ef-ficacy of the PAP protocol, with JH and VStiff demonstrating disparate responses in individual sub-jects.Such individual responses

  9. Mature and Fresh Surfaces on New-Born Asteroid Karin

    NASA Astrophysics Data System (ADS)

    Sasaki, S.; Sasaki, T.; Watanabe, J.; Sekiguchi, T.; Yoshida, F.; Ito, T.; Kawakita, H.; Fuse, T.; Takato, N.; Dermawan, B.

    2004-11-01

    We report a near-infrared (J, H, and K bands) spectroscopy of the brightest asteroid 832 Karin among the Karin cluster group, which was formed by collisional breakup only 5.8 million years ago. The spectroscopic observation was performed by the Subaru telescope with Cooled Infrared Spectrograph and Camera for OHS (CISCO) on 2003 September 14. To obtain a wide range spectrum, grisms named zJ (0.88-1.40 micron), JH (1.06-1.82 micron), and wK (1.85-2.51 micron) were used. We obtained 3 sets of spectra corresponding to the rotational phase 0.30-0.34, 0.35-0.38, and 0.45-0.50 in comparison with lightcurve observations. Near infrared (0.9-1.4micron) reflectance slope of the 1st set was twice as steep as that of later spectra. The range, where the most significant spectral change was detected, was observed both by zJ and JH bands. Gradual change of the spectral slope is detected through zJ(1st) - JH(1st) - zJ(2nd) - JH(2nd) data . We verified that spectra of a reference star SAO165395 (zJ) were not changed before the 1st set and after the 2nd set of Karin observation, which should remove the possibility that the spectral change was caused by instrumental or atmospheric (and hour angle) effect through the observation of the 1st set and the 2nd set of Karin. For different rotational phases of Karin, we derived different spectra such as a reddened spectrum like that of S-type asteroid and an un-reddened spectrum like that of ordinary chondrite. Karin would be an impact fragment which not only has new surface but also preserves old surface. Probably it would be one of cone-shaped fragments at low-velocity impact forming Karin cluster group. Our result supports the idea that S-type asteroids are parent bodies of ordinary chondrites.

  10. Plant-derived juvenile hormone III analogues and other sesquiterpenes from the stem bark of Cananga latifolia.

    PubMed

    Yang, Heejung; Kim, Hye Seong; Jeong, Eun Ju; Khiev, Piseth; Chin, Young-Won; Sung, Sang Hyun

    2013-10-01

    Juvenile hormone III (JH III) is a larval metamorphosis-regulating hormone present in most insect species. JH III was first isolated from the plant, Cyperus iria L., but the presence of JH III has not been reported in other plant species. In the present study, proof of the existence of JH III and its analogues from Cananga latifolia was established. From an aqueous MeOH extract of C. latifolia stem bark, six compounds were isolated along with nine known compounds. These were identified by using spectroscopic analyses as: (2E,6E,10R)-11-butoxy-10-hydroxy-3,7,11-trimethyldodeca-2,6-dienoic acid methyl ester, (2E,6E)-3,7,11-trimethyl-10-oxododeca-2,6-dienoic acid methyl ester, (2E)-3-methyl-5-[(1S,2R,6R)-1,2,6-trimethyl-3-oxocyclohexyl]-pent-2-enoic acid methyl ester, 1β-hydroxy-3-oxo-4β, 5α,7α-H-eudesmane 11-O-α-l-rhamnopyranoside, 4-epi-aubergenone 11-O-2',3'-di-O-acetyl-α-l-rhamnopyranoside and 4-epi-aubergenone 11-O-2',4'-di-O-acetyl-α-l-rhamnopyranoside. Three of the previously known compounds, (2E,6E,10R)-10-hydroxy-3,7,11-trimethyldodeca-2,6,11-trienoaic acid methyl ester, (2E,6E,10R)-10,11-dihydroxy-3,7,11-trimethyldodeca-2,6-dienoic acid and (2E,6S)-3-methyl-6-hydroxy-6-[(2R,5R)-5-(2-hydroxypropan-2-yl)-2-methyltetrahydrofuran-2-yl]-hex-2-enoaic acid methyl ester have now been found in a plant species. Ultra performance liquid chromatography-quadruple time-of-flight mass spectroscopy (UPLC-QTOF/MS) analysis of the chemical constituents of C. latifolia showed that several were predominant in the sub-fractions of a C. latifolia stem bark extract. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. Aging changes in the face

    MedlinePlus

    ... this page: //medlineplus.gov/ency/article/004004.htm Aging changes in the face To use the sharing ... face with age References Brodie SE, Francis JH. Aging and disorders of the eye. In: Fillit HM, ...

  12. TMJ disorders

    MedlinePlus

    ... conditions can cause TMJ symptoms, from arthritis to whiplash injuries. Experts who are specially trained in facial ... Garza I, Schwedt TJ, Robertson CE, Smith JH. Headache and other craniofacial pain. In: Daroff RB, Jankovic ...

  13. Changing Formulations of the Man-Environment Relationship in Anglo-American Geography

    ERIC Educational Resources Information Center

    Jeans, D. N.

    1974-01-01

    The following six formulations of the Man-Environment relationship have held successive favor in Geography since the 1900's: Economic Determinism, Possibilism, Cultural Relativism, the Landscape School, Perception of Environment, and Ecological Approach. (JH)

  14. Student Attitudes Toward Mental Illness

    ERIC Educational Resources Information Center

    Hare-Mustin, Rachel T.; Garvine, Richard

    1974-01-01

    Inquiry into the initial attitudes toward mental illness of students taking an abnormal psychology class indicates students' concerns and preconceptions and provides a basis for shaping the course to respond to student needs. (JH)

  15. Fungal Diseases: Ringworm Risk & Prevention

    MedlinePlus

    ... H, Parish LC. Manual of Medical Mycology, Blackwell Science, Cambridge 1995. p.36. Wilson EK, Deweber K, Berry JW, Wilckens JH. Cutaneous infections in wrestlers. Sports health 2013;5:423-37. Pecci M, Comeau ...

  16. Granulomatosis with polyangiitis

    MedlinePlus

    ... ncbi.nlm.nih.gov/pubmed/21372195 . Specks U, Merkel PA, Seo P, et al. Efficacy of remission- ... PA: Elsevier Saunders; 2016:chap 270. Stone JH, Merkel PA, Spiera R, et al. Rituximab versus cyclophosphamide ...

  17. Effects of Anti-diarrhoeal Herbs on Growth Performance, Nutrient Digestibility, and Meat Quality in Pigs

    PubMed Central

    Cho, J. H.; Zhang, S.; Kim, I. H.

    2012-01-01

    Two studies were conducted to investigate the effects of anti-diarrhoeal herbs on growth performance, nutrient digestibility, and meat quality in pigs. In Exp 1, 150 weanling-growing piglets (average BW = 7.5±0.24 kg, average age = 27±1 d) were allotted into one of the five dietary treatments, including: i) CON, basal diet, ii) DP, basal diet+1 g/kg date pits, iii) JH, basal diet+0.5 g/kg Japanese-honeysuckle, iv) HCT, basal diet+1 g/kg houttuynia cordata thunb, and v) LE, basal diet+1 g/kg laquer tree extract. From wk 0 to 5, the JH, HCT and LE groups presented higher (p<0.05) ADFI, ADG and gain/feed ratio (G/F) than CON and DP groups. During wk 5 to 10, Pigs fed JH, HCT and LE diets indicated higher (p<0.05) ADG and ADFI than the pigs fed CON and DP diets. During the entire experimental period, a significant increase of ADG appeared in JH, HCT and LE (p<0.05). Pigs fed JH, HCT and LE diets got a higher (p<0.05) ADFI than the pigs fed CON and DP diets. Pigs fed diets with supplementations of herb additives revealled lower (p<0.05) score of diarrhea pigs during d 2 to d 6 compared with pigs fed CON diet. In Exp 2, 60 growing-finishing barrows and gilts (average BW = 54.10±1.20 kg, average age = 54±3 d) were allotted to three treatments: i) CON, basal diet; ii) YG, basal diet+1 g/kg yellow ginger and iii) HR, basal dietary+1 g/kg hoantchy root, respectively. From wk 0 to 5, Dietary supplementation of YG and HR enhanced (p<0.05) ADG. No difference was found between YG and HR treatments. During, wk 5 to 10, ADG also was observed higher in YG and HR treatments than CON group (p<0.05). Additional, YG had the highest ADG (p<0.05) among treatments. There was always an increase of ADG in YG and HR (p<0.05) through all periods. HR treatment showed a lower (p<0.05) score of diarrhoeal pigs on d 1and d 2 compared with CON treatment. Pigs fed YG and HR diets had a higher (p<0.05) longissimus muscle area (LMA) than pigs fed CON diet. In conclusion, anti-diarrhoeal herbs can

  18. Neck x-ray

    MedlinePlus

    ... Van Goethem JW, Parizel PM. Imaging techniques and anatomy. In: Adam A, Dixon AK, Gillard JH, Schaefer-Prokop CM, eds. Grainger & Allison's Diagnostic Radiology: A Textbook of Medical Imaging . 6th ed. Philadelphia, PA: Elsevier ...

  19. Targeted therapy: questions to ask your doctor

    MedlinePlus

    ... References Garraway LA, Verweij J, Ballman KV. Precision oncology: an overview. J Clin Oncol . 2013;31(15): ... JH, Kastan MB, Tepper JE, eds. Abeloff's Clinical Oncology . 5th ed. Philadelphia, PA: Elsevier Saunders; 2014:chap ...

  20. Pancreatic islet cell tumor

    MedlinePlus

    ... JH, Kastan MB, Tepper JE, eds. Abeloff's Clinical Oncology . 5th ed. Philadelphia, PA: Elsevier Saunders; 2014:chap ... Cancer Network website. NCCN clinical practice guidelines in oncology (NCCN guidelines): pancreatic adenocarcinoma. Version 3.2017. www. ...

  1. Hepatocellular carcinoma

    MedlinePlus

    ... JH, Kastan MB, Tepper JE, eds. Abeloff's Clinical Oncology . 5th ed. Philadelphia, PA: Elsevier Saunders; 2014:chap ... Cancer Network website. NCCN clinical practice guidelines in oncology: hepatobiliary cancers. Version 3.2017. www.nccn.org/ ...

  2. Pancreatic cancer

    MedlinePlus

    ... JH, Kastan MB, Tepper JE, eds. Abeloff's Clinical Oncology . 5th ed. Philadelphia, PA: Elsevier Saunders; 2014:chap ... Cancer Network website. NCCN clinical practice guidelines in oncology: pancreatic adenocarcinoma. Version 1.2018. www.nccn.org/ ...

  3. When you have diarrhea

    MedlinePlus

    ... small meals throughout the day, instead of 3 big meals. Eat some salty foods, such as pretzels, ... Edition . 8th ed. Philadelphia, PA: Elsevier Saunders; 2015:chap 100. Shiller LR, Sellin JH. Diarrhea. In: Feldman ...

  4. Research Reports: Hallucinogens and Dissociative Drugs

    MedlinePlus

    ... 2001;54(1):27-29. Halpern JH, Sherwood AR, Hudson JI, Yurgelun-Todd D, Pope HG Jr. ... SMA) 14-4887. NSDUH Series H-49. Winstock AR, Kaar S, Borschmann R. Dimethyltryptamine (DMT): prevalence, user characteristics ...

  5. Polydactyly

    MedlinePlus

    ... The upper limb. In: Kliegman RM, Stanton BF, St. Geme JW, Schor NF, eds. Nelson Textbook of ... the hand. In: Azar FM, Beaty JH, Canale ST, eds. Campbell's Operative Orthopaedics . 13th ed. Philadelphia, PA: ...

  6. Thromboangiitis obliterans

    MedlinePlus

    ... or feet: Ultrasound of the extremity, called plethysmography Doppler ultrasound of the extremity Blood tests for other ... chap 79. Stone JH. The systemic vasculitudes. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine . 25th ...

  7. Head and face reconstruction

    MedlinePlus

    ... of the face. That is why sometimes a plastic surgeon (for skin and face) and a neurosurgeon ( ... Saunders; 2015:chap 24. McGrath MH, Pomerantz JH. Plastic surgery. In: Townsend CM Jr, Beauchamp RD, Evers ...

  8. Immunotherapy for cancer

    MedlinePlus

    ... 2017. Accessed February 15, 2018. Pardoll D. Cancer immunology. In: Niederhuber JE, Armitage JO, Doroshow JH, Kastan ... D.A.M. Editorial team. Related MedlinePlus Health Topics Cancer Immunotherapy Browse the Encyclopedia A.D.A. ...

  9. Geographic Education for Spaceship Earth

    ERIC Educational Resources Information Center

    Blachford, Kevin

    1974-01-01

    Six unsatisfactory ways of approaching geographic concepts in the classroom are presented. An alternative that would promote global-mindedness and the development of accurate mental maps in students is discussed in terms of teaching techniques. (JH)

  10. The Relation of Birth Order, Social Class, and Need Achievement to Independent Judgement

    ERIC Educational Resources Information Center

    Rhine, W. Ray

    1974-01-01

    This article reports an investigation in which the brith order, social class, and level of achievement arousal are the variables considered when fifth and sixth-grade girls make independent judgements in performing a set task. (JH)

  11. Genetics Home Reference: myoclonus-dystonia

    MedlinePlus

    ... Page Esapa CT, Waite A, Locke M, Benson MA, Kraus M, McIlhinney RA, Sillitoe RV, Beesley PW, ... PubMed Gerrits MC, Foncke EM, Koelman JH, Tijssen MA. Pediatric writer's cramp in myoclonus-dystonia: maternal imprinting ...

  12. Questions to ask your doctor before knee replacement

    MedlinePlus

    ... before; Before knee replacement - doctor questions; Before knee arthroplasty - what to ask your doctor ... Mihalko WM. Arthroplasty of the knee. In: Azar FM, Beaty JH, Canale ST, eds. Campbell's Operative Orthopaedics . 13th ed. Philadelphia, PA: ...

  13. Recent Growth of Aerial Photographic Interpretation/Remote Sensing in Geography in the United States

    ERIC Educational Resources Information Center

    Estes, John E.; Thaman, Konai

    1974-01-01

    This paper traces the history and growth of air photo interpretation and remote sensing within the field of geography. Courses offered in these fields, factors influencing growth, research findings, and professional geographic interest are discussed. (JH)

  14. Argentina: Its Physical-Cultural Backgrounds and Implications for United States Foreign Policy

    ERIC Educational Resources Information Center

    Vent, Herbert J.

    1974-01-01

    This article provides a short history of political events in Argentina and a look at the people, geography, and economy of the country in order to consider current relations between Argentina and the United States. (JH)

  15. Pubic lice

    MedlinePlus

    ... humans. Other types of lice include: Body lice Head lice You are at greater risk for pubic lice ... Images Crab louse, female Pubic louse-male Crab lice Head louse and pubic louse References Diaz JH. Lice ( ...

  16. Questions to ask your doctor after knee replacement

    MedlinePlus

    ... replacement - after - what to ask your doctor; Knee arthroplasty - after - what to ask your doctor ... Mihalko WM. Arthroplasty of the knee. In: Azar FM, Beaty JH, Canale ST, eds. Campbell's Operative Orthopaedics . 13th ed. Philadelphia, PA: ...

  17. Bleeding during cancer treatment

    MedlinePlus

    Doroshow JH. Approach to the patient with cancer. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 179. National Cancer Institute website. Chemotherapy and you: support for people ...

  18. Radiation therapy

    MedlinePlus

    Doroshow JH. Approach to the patient with cancer. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 179. National Cancer Institute. Radiation therapy and you: support for people ...

  19. Metastasis

    MedlinePlus

    Doroshow JH. Approach to the patient with cancer. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 179. Rankin EB. Erler J. Giaccia AJ. The cellular microenvironment and ...

  20. The Family and Bilingual Socialization: A Sociolinguistic Study of a Sample of Chinese Children in the United States

    ERIC Educational Resources Information Center

    Kuo, Eddie Chen-Yu

    1974-01-01

    The relationship among the family and the bilingual socialization of the child are explored in this sociolinguistic study of a sample of preschool Chinese children. The importance of the family as socializing agent is clarified. (Author/JH)

  1. Comparative proteomics and expression analysis of five genes in Epicauta chinensis larvae from the first to fifth instar.

    PubMed

    Li, Qiurong; Wang, Dun; Lv, Shumin; Zhang, Yalin

    2014-01-01

    Blister beetle is an important insect model for both medicinal and pure research. Previous research has mainly focused on its biology and biochemistry, but very little data is yet available in the molecular biology. This study uses differential proteomics technology to analyze the soluble proteins extracted from each of the 5 instars larvae of Epicauta chinensis. 42 of the differentially-expressed proteins were identified successfully by MALDI-TOF/TOF-MS. Some of these proteins' function and their expression profiles are analyzed. Our analysis revealed dynamics regulation of the following proteins: Axin-like protein pry-1 (APR-1), dihydrolipoyl dehydrogenase (DLD), vitellogenin (Vg) and lysozyme C (Lmz-S). APR-1 negatively regulates the Wnt signaling pathway. Its overexpression could result in embryo, leg, eye and ovary ectopica or malformation. DLD catalyzes the pyruvate into acetyl-CoA, the latter is the starting material of juvenile hormone (JH) and ipsdienol biosynthesis through the MVA pathway in insects. While Vg synthesis can be regulated by JH and stimulated by food factors. So DLD may affect the synthesis of JH, ipsdienol and Vg indirectly. The activity of lysozyme is an indicator of the immunity. Nutrition/food should be taken into account for its potential role during the development of larva in the future. Among the five genes and their corresponding proteins' expression, only hsc70 gene showed a good correspondence with the protein level. This reflects the fluctuating relationship between mRNA and protein levels.

  2. Countermovement-Jump-Phase Characteristics of Senior and Academy Rugby League Players.

    PubMed

    McMahon, John J; Murphy, Shannon; Rej, Sophie J E; Comfort, Paul

    2017-07-01

    Gross measures of countermovement-jump (CMJ) performance are commonly used to track maturational changes in neuromuscular function in rugby league (RL). The purpose of this study was to conduct both a gross and a more detailed temporal-phase analysis of the CMJ performances of senior and academy RL players, to provide greater insight into how neuromuscular function differs between these groups. Twenty senior and 14 academy (under-19) male RL players performed 3 maximal-effort CMJs on a force platform, with forward dynamics subsequently employed to allow gross performance measures and entire kinetic- and kinematic-time curves to be compared between groups. Jump height (JH), reactive strength index modified, concentric displacement, and relative concentric impulse (C-IMP) were the only gross measures that were greater for senior players (d = 0.58-0.91) than for academy players. The relative force- and displacement-time curves were similar between groups, but the relative power- and velocity-time curves were greater (d = 0.59-0.97) for the senior players at 94-96% and 89-100% of the total movement time, respectively. The CMJ distinguished between senior and academy RL players, with seniors demonstrating greater JH through applying a larger C-IMP and thus achieving greater velocity throughout the majority of the concentric phase and at takeoff. Therefore, academy RL players should train to improve triple (ie, ankle, knee, and hip) extension velocity during the CMJ to bring their JH scores in line with those attained by senior players.

  3. DNA methylation affects the lifespan of honey bee (Apis mellifera L.) workers - Evidence for a regulatory module that involves vitellogenin expression but is independent of juvenile hormone function.

    PubMed

    Cardoso-Júnior, Carlos A M; Guidugli-Lazzarini, Karina R; Hartfelder, Klaus

    2018-01-01

    The canonic regulatory module for lifespan of honey bee (Apis mellifera) workers involves a mutual repressor relationship between juvenile hormone (JH) and vitellogenin (Vg). Compared to vertebrates, however, little is known about a possible role of epigenetic factors. The full genomic repertoire of DNA methyltransferases (DNMTs) makes the honey bee an attractive emergent model for studying the role of epigenetics in the aging process of invertebrates, and especially so in social insects. We first quantified the transcript levels of the four DNMTs encoding genes in the head thorax and abdomens of workers of different age, showing that dnmt1a and dnmt3 expression is up-regulated in abdomens of old workers, whereas dnmt1b and dnmt2 are down-regulated in heads of old workers. Pharmacological genome demethylation by RG108 treatment caused an increase in worker lifespan. Next, we showed that the genomic DNA methylation status indirectly affects vitellogenin gene expression both in vitro and in vivo in young workers, and that this occurs independent of caloric restriction or JH levels, suggesting that a non-canonical circuitry may be acting in parallel with the JH/Vg module to regulate the adult life cycle of honey bee workers. Our data provide evidence that epigenetic factors play a role in regulatory networks associated with complex life history traits of a social insect. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Developmental and hormone-induced changes of mitochondrial electron transport chain enzyme activities during the last instar larval development of maize stem borer, Chilo partellus (Lepidoptera: Crambidae).

    PubMed

    VenkatRao, V; Chaitanya, R K; Naresh Kumar, D; Bramhaiah, M; Dutta-Gupta, A

    2016-12-01

    The energy demand for structural remodelling in holometabolous insects is met by cellular mitochondria. Developmental and hormone-induced changes in the mitochondrial respiratory activity during insect metamorphosis are not well documented. The present study investigates activities of enzymes of mitochondrial electron transport chain (ETC) namely, NADH:ubiquinone oxidoreductase or complex I, Succinate: ubiquinone oxidoreductase or complex II, Ubiquinol:ferricytochrome c oxidoreductase or complex III, cytochrome c oxidase or complex IV and F 1 F 0 ATPase (ATPase), during Chilo partellus development. Further, the effect of juvenile hormone (JH) analog, methoprene, and brain and corpora-allata-corpora-cardiaca (CC-CA) homogenates that represent neurohormones, on the ETC enzyme activities was monitored. The enzymatic activities increased from penultimate to last larval stage and thereafter declined during pupal development with an exception of ATPase which showed high enzyme activity during last larval and pupal stages compared to the penultimate stage. JH analog, methoprene differentially modulated ETC enzyme activities. It stimulated complex I and IV enzyme activities, but did not alter the activities of complex II, III and ATPase. On the other hand, brain homogenate declined the ATPase activity while the injected CC-CA homogenate stimulated complex I and IV enzyme activities. Cumulatively, the present study is the first to show that mitochondrial ETC enzyme system is under hormone control, particularly of JH and neurohormones during insect development. Copyright © 2015 Elsevier Inc. All rights reserved.

  5. Responses of Chinese University Students to the Thematic Apperception Test

    ERIC Educational Resources Information Center

    Han, Elizabeth Yeo-hsien

    1974-01-01

    Murray's original Thematic Apperception Test (TAT), Cards I to XX, was administered in two sessions to 80 Chinese male and female undergraduate students at the National Taiwan Normal University. The results are compared with American averages. (Author/JH)

  6. Genetics Home Reference: spastic paraplegia type 3A

    MedlinePlus

    ... on PubMed or Free article on PubMed Central Blackstone C. Cellular pathways of hereditary spastic paraplegia. Annu ... PP, Soderblom C, Tao-Cheng JH, Stadler J, Blackstone C. SPG3A protein atlastin-1 is enriched in ...

  7. Food and History: Teaching Social History Through the Study of Cuisine Patterns

    ERIC Educational Resources Information Center

    Gordon, Bertram M.

    1974-01-01

    Some interesting relationships between food and history -- working women and convenience food, exploration and the importation of new vegetables, reliance on potatoes and Irish emigration -- are suggested to indicate the usefulness of exploring history through eating habits. (JH)

  8. Stools - floating

    MedlinePlus

    ... diagnosis. Alternative Names Floating stools Images Lower digestive anatomy References Schiller LR, Sellin JH. Diarrhea. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease . 10th ed. Philadelphia, PA: Elsevier Saunders; 2016: ...

  9. 9. Charles B. Wheelock, Photographer ca. 1890 'VIEW OF WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Charles B. Wheelock, Photographer ca. 1890 'VIEW OF WEST AUSTIN TAKEN FROM CAPITOL DOME' SHOWING J.H. HOUGHTON HOUSE WITH TAYLOR-HUNNICUTT HOUSE JUST BEHIND - John H. Houghton House, 307 West Twelfth Street, Austin, Travis County, TX

  10. Publications - PDF 98-39 | Alaska Division of Geological & Geophysical

    Science.gov Websites

    content DGGS PDF 98-39 Publication Details Title: Rock geochemistry from the Manley mining district ., Bundtzen, T.K., Newberry, R.J., Dover, J.H., and Blodgett, R.B., 1998, Rock geochemistry from the Manley

  11. Radiation therapy -- skin care

    MedlinePlus

    ... References Doroshow JH. Approach to the patient with cancer. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 179. National Cancer Institute website. Radiation therapy and you: support for ...

  12. Cancer treatments

    MedlinePlus

    ... 2017. Doroshow JH. Approach to the patient with cancer. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 179. National Cancer Institute website. Types of cancer treatment. www.cancer. ...

  13. Ligand binding pocket function of drosophila USP is necessary for metamorphosis

    USDA-ARS?s Scientific Manuscript database

    The widely accepted paradigm that epoxidized methyl farnesoates (“juvenile hormones,” JHs) are the principle sesquiterpenoid hormones regulating insect metamorphosis was assessed in Drosophila melanogaster. GC-MS analysis showed that methyl farnesoate, rather than methyl epoxyfarnesoate (= JH III), ...

  14. The Child Welfare Agency as School Consultant

    ERIC Educational Resources Information Center

    Schild, Judith S.; And Others

    1976-01-01

    Describes the School Consultation Program offered by the Jewish Children's Bureau (JCB) to the special education personnel of four elementary schools in a suburban school district. Discusses problems and special techniques of applying child welfare to school consultation. (JH)

  15. Theories of Motivation--Borrowing the Best.

    ERIC Educational Resources Information Center

    Terpstra, David E.

    1979-01-01

    Five theories of motivation are discussed: Maslow's Need Hierarchy, Herzberg's dual-factor or motivation-hygiene theory, goal setting or task motivation, expectancy/valence-theory (also known as instrumentality theory, valence-instrumentality-expectancy theory, or expectancy theory), and reinforcement. (JH)

  16. Publications - PDF 97-29G | Alaska Division of Geological & Geophysical

    Science.gov Websites

    content DGGS PDF 97-29G Publication Details Title: Rock geochemistry from the Rampart mining district , K.H., Bundtzen, T.K., Newberry, R.J., Dover, J.H., and Blodgett, R.B., 1997, Rock geochemistry from

  17. Genetics Home Reference: 16p11.2 deletion syndrome

    MedlinePlus

    ... Schuurs-Hoeijmakers JH, van Haeringen A, Fransen van de Putte DE, Anderlid BM, Lundin J, Lapunzina P, Pérez Jurado ... Marshall CR, Scherer SW. Phenotypic spectrum associated with de novo and inherited deletions and duplications at 16p11. ...

  18. Human Revolution: Confronting the Realities of Them and Us

    ERIC Educational Resources Information Center

    Jourard, Sidney M.

    1970-01-01

    The nation's schools and universities have been largely engaged in training and indoctrination as opposed to"education." An educational system is needed that provides time and opportunities for self-renewal and personal growth for both teachers and students. (JH)

  19. 77 FR 17505 - Morris W. Cochran, M.D.: Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... as cocaine * * * or marijuana at that time.'' Id. at 483. However, JB's file does not contain the... marijuana use. GX 5R, at 12. Respondent believed his care of JH was appropriate. Tr. 492. KP On June 2, 2010...

  20. Cloning and expressing a highly functional and substrate specific farnesoic acid o-methyltransferase from the Asian citrus psyllid (Diaphorina citri Kuwayama)

    USDA-ARS?s Scientific Manuscript database

    The Asian citrus psyllid, Diaphorina citri, transmits a phloem-limited bacterium, Candidatus Liberibacter asiaticus that causes citrus greening disease. Because juvenile hormone (JH) plays an important role in adult and nymphal development, we studied the final steps in juvenile hormone biosynthesis...

  1. U.S. EPA, Pesticide Product Label, MORGRO EPTAM 2.3 GRANULAR, 08/11/1967

    EPA Pesticide Factsheets

    2011-04-13

    ... i_. '" v_"", -- fpc,;:;. Li..l~~ :x.- ~.fttH~ ~n:t tlrlilP Ju-,ln~ tli( fll ..... l:li jt:.:;. 1\\6 111 .n~ Ml'Clur,;~ ;-OW''':lti, '_:~es . .lhr~Jh~~ ar.~ ~r:OlllJ "-('v._r ..... ...

  2. The Impact of Social Cues on Children's Behavior

    ERIC Educational Resources Information Center

    Dweck, Carol S.; And Others

    1976-01-01

    Introduces purpose of symposium: to discuss research which explores the factors determining how a child, faced with obtaining some goal or fulfilling some criterion of performance, responds to given instructional or evaluative cues. Delineates variety of research strategies employed. (JH)

  3. Cancer treatment - dealing with pain

    MedlinePlus

    Byker GL, Leskuski D. Nonpharmacologic management of pain. In: Berger AM, Shuster JL, Von Roenn JH, eds. Principles and Practice of Palliative Care and Supportive Oncology . 4th ed. Philadelphia, PA: Lippincott Williams & Wilkins; 2013:chap 3. Grossman SA, Nesbit S. ...

  4. Genetics Home Reference: Schimke immuno-osseous dysplasia

    MedlinePlus

    ... Smithson SF, Sloan EA, Lou S, Huang Y, Choi K, Lücke T, Alpay H, André JL, Asakura Y, ... Collard L, Cordeiro I, Cormier-Daire V, Cransberg K, Cutka K, Deschenes G, Ehrich JH, Fründ S, Georgaki ...

  5. The Co-Op Industrial Education Experiment, 1900-1917

    ERIC Educational Resources Information Center

    McBride, Paul W.

    1974-01-01

    Cooperative education or industrial education, initiated to create an efficient work force, was exploitive of both students and the general public. It practically endentured students, educated them to docility, and procured public tax funds to subsidize the industries involved. (JH)

  6. Curriculum Design in Canadian Studies

    ERIC Educational Resources Information Center

    Love, James H.

    1974-01-01

    Curriculum development in Canadian studies can be based effectively in the attainment of skills through an inquiry approach. Emphasis on skills and inquiry would implement the following criteria: relevance to students and school systems, interdisciplinary content, and evaluateable goals. (JH)

  7. Pituitary tumor

    MedlinePlus

    ... during the person's lifetime. The pituitary is part of the endocrine system. The pituitary helps control the release of hormones ... Hollander AB, Alonso-Basanta M, et al. Cancer of the central nervous system. In: Niederhuber JE, Armitage JO, Doroshow JH, Kastan ...

  8. Sinus x-ray

    MedlinePlus

    Paranasal sinus radiography; X-ray - sinuses ... sinus x-ray is taken in a hospital radiology department. Or the x-ray may be taken ... Brown J, Rout J. ENT, neck, and dental radiology. In: Adam A, Dixon AK, Gillard JH, Schaefer- ...

  9. The four hexamerin genes in the honey bee: structure, molecular evolution and function deduced from expression patterns in queens, workers and drones.

    PubMed

    Martins, Juliana R; Nunes, Francis M F; Cristino, Alexandre S; Simões, Zilá L P; Bitondi, Márcia M G

    2010-03-26

    Hexamerins are hemocyanin-derived proteins that have lost the ability to bind copper ions and transport oxygen; instead, they became storage proteins. The current study aimed to broaden our knowledge on the hexamerin genes found in the honey bee genome by exploring their structural characteristics, expression profiles, evolution, and functions in the life cycle of workers, drones and queens. The hexamerin genes of the honey bee (hex 70a, hex 70b, hex 70c and hex 110) diverge considerably in structure, so that the overall amino acid identity shared among their deduced protein subunits varies from 30 to 42%. Bioinformatics search for motifs in the respective upstream control regions (UCRs) revealed six overrepresented motifs including a potential binding site for Ultraspiracle (Usp), a target of juvenile hormone (JH). The expression of these genes was induced by topical application of JH on worker larvae. The four genes are highly transcribed by the larval fat body, although with significant differences in transcript levels, but only hex 110 and hex 70a are re-induced in the adult fat body in a caste- and sex-specific fashion, workers showing the highest expression. Transcripts for hex 110, hex 70a and hex70b were detected in developing ovaries and testes, and hex 110 was highly transcribed in the ovaries of egg-laying queens. A phylogenetic analysis revealed that HEX 110 is located at the most basal position among the holometabola hexamerins, and like HEX 70a and HEX 70c, it shares potential orthology relationship with hexamerins from other hymenopteran species. Striking differences were found in the structure and developmental expression of the four hexamerin genes in the honey bee. The presence of a potential binding site for Usp in the respective 5' UCRs, and the results of experiments on JH level manipulation in vivo support the hypothesis of regulation by JH. Transcript levels and patterns in the fat body and gonads suggest that, in addition to their primary

  10. Escherichia coli Heat-Stable Enterotoxin Mediates Na+/H+ Exchanger 4 Inhibition Involving cAMP in T84 Human Intestinal Epithelial Cells.

    PubMed

    Beltrán, Ana R; Carraro-Lacroix, Luciene R; Bezerra, Camila N A; Cornejo, Marcelo; Norambuena, Katrina; Toledo, Fernando; Araos, Joaquín; Pardo, Fabián; Leiva, Andrea; Sanhueza, Carlos; Malnic, Gerhard; Sobrevia, Luis; Ramírez, Marco A

    2015-01-01

    The enterotoxigenic Escherichia coli strains lead to diarrhoea in humans due to heat-labile and heat-stable (STa) enterotoxins. STa increases Cl-release in intestinal cells, including the human colonic carcinoma T84 cell line, involving increased cGMP and membrane alkalization due to reduced Na+/H+ exchangers (NHEs) activity. Since NHEs modulate intracellular pH (pHi), and NHE1, NHE2, and NHE4 are expressed in T84 cells, we characterized the STa role as modulator of these exchangers. pHi was assayed by the NH4Cl pulse technique and measured by fluorescence microscopy in BCECF-preloaded cells. pHi recovery rate (dpHi/dt) was determined in the absence or presence of 0.25 μmol/L STa (30 minutes), 25 μmol/L HOE-694 (concentration inhibiting NHE1 and NHE2), 500 μmol/L sodium nitroprusside (SNP, spontaneous nitric oxide donor), 100 μmol/L dibutyryl cyclic GMP (db-cGMP), 100 nmol/L H89 (protein kinase A inhibitor), or 10 μmol/L forskolin (adenylyl cyclase activator). cGMP and cAMP were measured in cell extracts by radioimmunoassay, and buffering capacity (ßi) and H+ efflux (JH+) was determined. NHE4 protein abundance was determined by western blotting. STa and HOE-694 caused comparable reduction in dpHi/dt and JH+ (~63%), without altering basal pHi (range 7.144-7.172). STa did not alter ßi value in a range of 1.6 pHi units. The dpHi/dt and JH+ was almost abolished (~94% inhibition) by STa + HOE-694. STa effect was unaltered by db-cGMP or SNP. However, STa and forskolin increased cAMP level. STa-decreased dpHi/dt and JH+ was mimicked by forskolin, and STa + HOE-694 effect was abolished by H89. Thus, incubation of T84 cells with STa results in reduced NHE4 activity leading to a lower capacity of pHi recovery requiring cAMP, but not cGMP. STa effect results in a causal phenomenon (STa/increased cAMP/increased PKA activity/reduced NHE4 activity) ending with intracellular acidification that could have consequences in the gastrointestinal cells function promoting human

  11. The Acute Effects of Back Squats on Vertical Jump Performance in Men and Women

    PubMed Central

    Witmer, Chad A.; Davis, Shala E.; Moir, Gavin L.

    2010-01-01

    The aim of the present study was to investigate the acute effects of performing back squats on subsequent performance during a series of vertical jumps in men and women. Twelve men and 12 women were tested on three separate occasions, the first of which was used to determine their 1-repetition maximum (1-RM) parallel back squat. Following this, subjects performed a potentiation and a control treatment in a counterbalanced order. The potentiation treatment culminated with subjects performing parallel back squats with a load equivalent to 70% 1- RM for three repetitions, following which they performed one countermovement vertical jump (CMJ) for maximal height every three minutes for a total of 10 jumps. During the control treatment, subjects performed only the CMJs. Jump height (JH) and vertical stiffness (VStiff) were calculated for each jump from the vertical force signal recorded from a force platform. There were no significant changes in JH or VStiff following the treatments and no significant differences in the responses between men and women (p > 0.05). Correlations between normalized 1-RM back squat load and the absolute change in JH and VStiff were small to moderate for both men and women, with most correlations being negative. Large variations in response to the back squats were noted in both men and women. The use of resistance exercises performed prior to a series of vertical jumps can result in improvements in performance in certain individuals, although the gains tend to be small and dependent upon the mechanical variable measured. There does not seem to be any differences between men and women in the response to dynamic potentiation protocols. Key points Substantial individual responses were noted in both men and women in response to the PAP protocol used in the present study. The choice of dependent variable influences the ef-ficacy of the PAP protocol, with JH and VStiff demonstrating disparate responses in individual sub-jects.Such individual

  12. Promoting mobility and reducing length of stay in hospitalized general medicine patients: A quality-improvement project.

    PubMed

    Hoyer, Erik H; Friedman, Michael; Lavezza, Annette; Wagner-Kosmakos, Kathleen; Lewis-Cherry, Robin; Skolnik, Judy L; Byers, Sherrie P; Atanelov, Levan; Colantuoni, Elizabeth; Brotman, Daniel J; Needham, Dale M

    2016-05-01

    To determine whether a multidisciplinary mobility promotion quality-improvement (QI) project would increase patient mobility and reduce hospital length of stay (LOS). Implemented using a structured QI model, the project took place between March 1, 2013 and March 1, 2014 on 2 general medicine units in a large academic medical center. There were 3352 patients admitted during the QI project period. The Johns Hopkins Highest Level of Mobility (JH-HLM) scale, an 8-point ordinal scale ranging from bed rest (score = 1) to ambulating ≥250 feet (score = 8), was used to quantify mobility. Changes in JH-HLM scores were compared for the first 4 months of the project (ramp-up phase) versus 4 months after project completion (post-QI phase) using generalized estimating equations. We compared the relative change in median LOS for the project months versus 12 months prior among the QI units, using multivariable linear regression analysis adjusting for 7 demographic and clinically relevant variables. Comparing the ramp-up versus post-QI phases, patients reaching JH-HLM's ambulation status increased from 43% to 70% (P < 0.001), and patients with improved JH-HLM mobility scores between admission and discharge increased from 32% to 45% (P < 0.001). For all patients, the QI project was associated with an adjusted median LOS reduction of 0.40 (95% confidence interval [CI]: -0.57 to -0.21, P < 0.001) days compared to 12 months prior. A subgroup of patients expected to have a longer LOS (expected LOS >7 days), were associated with a significantly greater adjusted median reduction in LOS of 1.11 (95% CI: -1.53 to -0.65, P < 0.001) days. Increased mobility was not associated with an increase in injurious falls compared to 12 months prior on the QI units (P = 0.73). Active prevention of a decline in physical function that commonly occurs during hospitalization may be achieved with a structured QI approach. In an adult medicine population, our QI project was associated with improved

  13. Aedes aegypti juvenile hormone acid methyl transferase, the ultimate enzyme in the biosynthetic pathway of juvenile hormone III, exhibits substrate control

    USDA-ARS?s Scientific Manuscript database

    We report on the cloning, sequencing, characterization, 3D modeling and docking of Aedes aegypti juvenile hormone acid methyl transferase (AeaJHAMT), the enzyme that converts juvenile hormone acid (JHA) into juvenile hormone (JH). Purified recombinant AeaJHAMT was extensively characterized for enzym...

  14. A Parent's Guide to the Social Studies

    ERIC Educational Resources Information Center

    Roselle, Daniel

    1974-01-01

    This insert, an excerpt from a forthcoming National Council for the Social Studies booklet, explains to parents the meaning, functions, and programs of social studies and includes suggestions of ways parents may help their children get the most out of social studies. (JH)

  15. CONSISTENT INFLAMMATORY RESPONSE FOLLOWING EXPOSURE TO CONCENTRATED AMBIENT PARTICLES (CAPS) DURING FALL SEASON IN WISTAR-KYOTO RATS

    EPA Science Inventory

    CONSISTENT INFLAMMATORY RESPONSE FOLLOWING EXPOSURE TO CONCENTRATED AMBIENT PARTICLES (CAPs) DURING FALL SEASON IN WISTAR-KYOTO RATS.
    UP Kodavanti, MC Schladweiler, AD Ledbetter, LC Walsh, PS Gilmour, MI Gilmour, WP Watkinson, JP Nolan, JH Richards, D Andrews, DL Costa. US EPA...

  16. Theoretical, Practical and Personal Domains in the Curriculum of Teacher Education

    ERIC Educational Resources Information Center

    Ingram, James B.

    1974-01-01

    This survey of teacher trainers in Rhodesia considers their intentions in teaching with respect to three domains of teacher education -- theoretical, practical, and personal. The study assumes that the effectiveness of a curriculum is ultimately dependent on the intentions of its teachers. (JH)

  17. The Relationship Between Economic Understanding and the Social Studies Curriculum

    ERIC Educational Resources Information Center

    Boddy, Edward; Tocco, Thomas S.

    1974-01-01

    The purpose of the study was to describe the contribution made by the social studies curriculum, in general, and the Americanism vs. Communism course in particular, to the development of overall economic understanding among high school seniors in Southwest Florida. (Author/JH)

  18. Endocrine control of exaggerated traits in rhinoceros beetles

    USDA-ARS?s Scientific Manuscript database

    Juvenile hormone (JH) is a key insect growth regulator involved in modulating phenotypically plastic traits in insects such as caste determination in eusocial species, wing polymorphisms in aphids, and mandible size in stag beetle. Male stag beetles have sexually-dimorphic, condition-dependent expre...

  19. Socio-Economic Influences on School Attendance: A study of a Canadian Country in 1871

    ERIC Educational Resources Information Center

    Denton, Frank T.; George, Peter J.

    1974-01-01

    Cross-section regression techniques are applied to census data from the year 1871 in Wentworth County, Ontario, to ascertain the influence of variables such as age, origin, locale -- urban or rural -- and family size on school attendance among children 10-16. (JH)

  20. Public Policy and Private Enterprise in the Development of Flood Plains: A Laboratory Exercise in Physical Geography

    ERIC Educational Resources Information Center

    Nunnally, Nelson R.; And Others

    1974-01-01

    This activity is designed to introduce college students to the concept of floods as natural hazards, to flood frequency analysis, to hazard adjustment, and to the mechanics of public policy formulation through a six hour laboratory exercise, culminating in a simulation game. (JH)

  1. GENETIC INFLUENCES ON IN VTIRO PARTICULATE MATTER-INDUCED AIRWAY EPITHELIAL INJURY AND INFLAMMATORY MEDIATOR RELEASE

    EPA Science Inventory

    GENETIC INFLUENCES ON IN VITRO PARTICULATE MATTER-INDUCED AIRWAY EPITHELIAL INJURY AND INFLAMMATORY MEDIATOR RELEASE.
    JA Dye, JH Richards, DA Andrews, UP Kodavanti. US EPA, RTP, NC, USA.

    Particulate matter (PM) air pollution is capable of damaging the airway epitheli...

  2. Elements of Warfare in the Sixth- and Seventh-Grade Physics Course

    ERIC Educational Resources Information Center

    Enokhovich, A. S.

    1973-01-01

    Mechanical movement, pressure, floating bodies, heat phenomena, electrical phenomena, when applied to military problems "for the patriotic edification and military instruction of the children" excite interest and place theory on concrete foundations. Examples of applied physical concepts follow in this article. (Author/JH)

  3. VARIATION IN GROWTH, LIPID CLASS AND FATTY ACID COMPOSITION OF THE MUD CRAB, RHITHROPANOPEUS HARRISII (GOULD) DURING LARVAL DEVELOPMENT FOLLOWING EXPOSURE TO AN INSECT JUVENILE HORMONE ANALOG (FENOXYCARB(R))

    EPA Science Inventory

    This study examines the effects of fenoxycarb?, an insect juvenile hormone (JH) analog, on larval growth, and lipid class and fatty acid composition in first crabs of the mud crab Rhithropanopeus harrisii reared through total larval development in nominal water concentrations fr...

  4. Juvenile hormone mediates developmental integration between exaggerated traits and supportive traits in the horned flour beetle Gnatocerus cornutus.

    PubMed

    Okada, Yasukazu; Gotoh, Hiroki; Miura, Toru; Miyatake, Takahisa; Okada, Kensuke

    2012-07-01

    Sexually selected exaggerated traits are often coupled with modifications in other nontarget traits. In insects with weapons, enlargements of nontarget characters that functionally support the weapon often occur (i.e. supportive traits). The support of sexual traits requires developmental coordination among functionally related multiple traits-an explicit example of morphological integration. The genetic theory predicts that developmental integration among different body modules, for which development is regulated via different sets of genes, is likely to be coordinated by pleiotropic factors. However, the developmental backgrounds of morphological integrations are largely unknown. We tested the hypothesis that the juvenile hormone (JH), as a pleiotropic factor, mediates the integration between exaggerated and supportive traits in an armed beetle Gnatocerus cornutus. During combat, males of this beetle use exaggerated mandibles to lift up their opponents with the supportive traits, that is, the head and prothoracic body parts. Application of methoprene, a JH analog (JHA), during the larval to prepupal period, induced the formation of large mandibles relative to the body sizes in males. Morphometric examination of nontarget traits elucidated an increase in the relative sizes of supportive traits, including the head and prothoracic body parts. In addition, reductions in the hind wing area and elytra length, which correspond to flight and reproductive abilities, were detected. Our findings are consistent with the genetic theory and support the idea that JH is a key pleiotropic factor that coordinates the developmental integration of exaggerated traits and supportive characters, as well as resource allocation trade-offs. © 2012 Wiley Periodicals, Inc.

  5. The essence of insect metamorphosis and aging: electrical rewiring of cells driven by the principles of juvenile hormone-dependent Ca(2+)-homeostasis.

    PubMed

    De Loof, Arnold; De Haes, Wouter; Janssen, Tom; Schoofs, Liliane

    2014-04-01

    In holometabolous insects the fall to zero of the titer of Juvenile Hormone ends its still poorly understood "status quo" mode of action in larvae. Concurrently it initiates metamorphosis of which the programmed cell death of all internal tissues that actively secrete proteins, such as the fat body, midgut, salivary glands, prothoracic glands, etc. is the most drastic aspect. These tissues have a very well developed rough endoplasmic reticulum, a known storage site of intracellular Ca(2+). A persistent high [Ca(2+)]i is toxic, lethal and causal to apoptosis. Metamorphosis becomes a logical phenomenon if analyzed from: (1) the causal link between calcium toxicity and apoptosis; (2) the largely overlooked fact that at least some isoforms of Ca(2+)-ATPases have a binding site for farnesol-like endogenous sesquiterpenoids (FRS). The Ca(2+)-ATPase blocker thapsigargin, like JH a sesquiterpenoid derivative, illustrates how absence of JH might work. The Ca(2+)-homeostasis system is concurrently extremely well conserved in evolution and highly variable, enabling tissue-, developmental-, and species specificity. As long as JH succeeds in keeping [Ca(2+)]i low by keeping the Ca(2+)-ATPases pumping, it acts as "the status quo" hormone. When it disappears, its various inhibitory effects are lifted. The electrical wiring system of cells, in particular in the regenerating tissues, is subject to change during metamorphosis. The possibility is discussed that in vertebrates an endogenous farnesol-like sesquiterpenoid, probably farnesol itself, acts as a functional, but hitherto completely overlooked Juvenile anti-aging "Inbrome", a novel concept in signaling. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. Molecular characterization of the gene feminizer in the stingless bee Melipona interrupta (Hymenoptera: Apidae) reveals association to sex and caste development.

    PubMed

    Brito, Diana V; Silva, Carlos Gustavo N; Hasselmann, Martin; Viana, Luciana S; Astolfi-Filho, Spartaco; Carvalho-Zilse, Gislene A

    2015-11-01

    In highly eusocial insects, development of reproductive traits are regulated not only by sex determination pathway, but it also depends on caste fate. The molecular basis of both mechanisms in stingless bees and possible interaction with each other is still obscure. Here, we investigate sex determination in Melipona interrupta, focusing on characterization and expression analysis of the feminizer gene (Mi-fem), and its association to a major component of caste determination, the juvenile hormone (JH). We present evidence that Mi-fem mRNA is sex-specifically spliced in which only the female splice variant encodes the full length protein, following the same principle known for other bee species. We quantified Mi-fem expression among developmental stages, sexes and castes. Mi-fem expression varies considerably throughout development, with higher expression levels in embryos. Also, fem levels in pupae and newly emerged adults were significantly higher in queens than workers and males. Finally, we ectopically applied JH in cocoon spinning larvae, which correspond to the time window where queen/worker phenotypes diverge. We observed a significantly increase in Mi-fem expression compared to control groups. Since up to 100% of females turn into queens when treated with JH (while control groups are composed mainly of workers), we propose that fem might act to regulate queens' development. Our findings provide support for the conserved regulatory function of fem in Melipona bees and demonstrate a significant correlation between key elements of sex and caste determination pathways, opening the avenue to further investigate the molecular basis of these complex traits. Copyright © 2015 Elsevier Ltd. All rights reserved.

  7. Differential gene expression underlying ovarian phenotype determination in honey bee, Apis mellifera L., caste development.

    PubMed

    Lago, Denyse Cavalcante; Humann, Fernanda Carvalho; Barchuk, Angel Roberto; Abraham, Kuruvilla Joseph; Hartfelder, Klaus

    2016-12-01

    Adult honey bee queens and workers drastically differ in ovary size. This adult ovary phenotype difference becomes established during the final larval instar, when massive programmed cell death leads to the degeneration of 95-99% of the ovariole anlagen in workers. The higher juvenile hormone (JH) levels in queen larvae protect the ovaries against such degeneration. To gain insights into the molecular architecture underlying this divergence critical for adult caste fate and worker sterility, we performed a microarray analysis on fourth and early fifth instar queen and worker ovaries. For the fourth instar we found nine differentially expressed genes (DEGs) with log 2 FC > 1.0, but this number increased to 56 in early fifth-instar ovaries. We selected 15 DEGs for quantitative PCR (RT-qPCR) analysis. Nine differed significantly by the variables caste and/or development. Interestingly, genes with enzyme functions were higher expressed in workers, while those related to transcription and signaling had higher transcript levels in queens. For the RT-qPCR confirmed genes we analyzed their response to JH. This revealed a significant up-regulation for two genes, a short chain dehydrogenase reductase (sdr) and a heat shock protein 90 (hsp90). Five other genes, including hsp60 and hexamerin 70b (hex70b), were significantly down-regulated by JH. The sdr gene had previously come up as differentially expressed in other transcriptome analyses on honey bee larvae and heat shock proteins are frequently involved in insect hormone responses, this making them interesting candidates for further functional assays. Copyright © 2016. Published by Elsevier Ltd.

  8. Neural Responses to Injury: Prevention, Protection, and Repair.

    DTIC Science & Technology

    1998-10-01

    opioid-sensitive circuitry by electroacupuncture can suppress c-fos expression (21). Anesthetic agents and system- ically administered morphine can...ders Co., 1995, pp 397-460. 21. Lee JH, Beitz AJ: Electroacupuncture modifies the expression of c-fos in the spinal cord induced by noxious

  9. Hormonal-Pheromonal Interrelationships in Ticks and Parasitic Mites.

    DTIC Science & Technology

    1986-12-01

    variabilis (Say). J. Parasitol. 1981 Oliver, J.H., Jr. Sex chromosomes, parthenogenesis , and polyploidy in ticks. In Atchley,. W.R. and D.S. Woodruff...reproduces oisexually, although there is a tendency for parthenogenesis in some individual females. The genetics of parthenogenesis is not understood

  10. METAL-INDUCED LATE PULMONARY INJURY IS REDUCED BY OZONE (O3) COEXPOSURE

    EPA Science Inventory

    METAL-INDUCED LATE PULMONARY INJURY IS REDUCED BY OZONE (O3) COEXPOSURE. UP Kodavanti, MCJ Schladweiler, WP Watkinson, JP Nolan, PA Evansky, ER Lappi, G Ross, JH Richards, and DL Costa. NHEERL, ORD, US Environmental Protection Agency, Research Triangle Park, NC USA.
    Ambient ...

  11. DOSIMETRY MODELING OF INHALED FORMALDEHYDE: BINNING NASAL FLUX PREDICTIONS FOR QUANTITATIVE RISK ASSESSMENT

    EPA Science Inventory

    Dosimetry Modeling of Inhaled Formaldehyde: Binning Nasal Flux Predictions for Quantitative Risk Assessment. Kimbell, J.S., Overton, J.H., Subramaniam, R.P., Schlosser, P.M., Morgan, K.T., Conolly, R.B., and Miller, F.J. (2001). Toxicol. Sci. 000, 000:000.

    Interspecies e...

  12. Assessing Organization Culture Readiness for Knowledge Management Implementation: The Case of Aeronautical Systems Center Directorate of Contracting

    DTIC Science & Technology

    2003-03-01

    effective change management techniques (Gibson, Ivancevich , & Donnelly, 2001). Based on case study 15 research, KM projects require more...Englewood Cliffs, NJ: Prentice Hall. 1986. Gibson, J.L., J.M. Ivancevich , and J.H. Donnelly Jr. Organizations. (10th ed.). Boston: Irwin McGraw-Hill

  13. Education and Professional Life Styles: Law and Medicine in the Nineteenth Century

    ERIC Educational Resources Information Center

    Johnson, William R.

    1974-01-01

    The history of medical and legal professional education in the 1800's is directly related to the social and intellectual context of the respective professions. Doctors required the establishment of a recognized "school" of medicine and lawyers established legitimacy through competent court room technique. (JH)

  14. A World-Wide Overview of Migratory Movements. The Education of Migrant Workers -- Where Do We Stand?

    ERIC Educational Resources Information Center

    Blanchard, Francis

    1974-01-01

    A survey of world migration patterns prefaces a declaration of educational problems and ways of solving them as viewed by the International Labour Organization (ILO). The problems are conceptualized on the socio-cultural and occupational levels and involve both the worker and his family. (JH)

  15. Technological Revolutions: Some Implications for the Social Studies

    ERIC Educational Resources Information Center

    Kane, Paul W.

    1975-01-01

    The advantages of a social studies program, centered on the various technological revolutions -- human, agricultural, urban, industrial, and humanizing -- are a multi-disciplinary approach, the use of inquiry skills, wide coverage of history and nations through a framework of concepts, and discussion of ethnocentrism. (JH)

  16. 40 CFR 73.10 - Initial allocations for phase I and phase II.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 1557 Shawnee 10 9902 268 Maryland C.P. Crane 1 10058 272 2 8987 243 Chalk Point 1 21333 577 2 23690 640 Morgantown 1 34332 928 2 37467 1013 Michigan J.H. Campbell 1 18773 507 2 22453 607 Minnesota High Bridge 6...

  17. The University -- Old Ghosts and New

    ERIC Educational Resources Information Center

    Perkins, James A.

    1974-01-01

    Successful outcomes of the immediate past must not overshadow four challenges in the future for the university -- the demand for equal education; the demand for public accountability; political and military nationalism combined with cultural pluralism; and limited resources for food, fuel, and space. (JH)

  18. Ultra-endurance sports have no negative impact on indices of arterial stiffness.

    PubMed

    Radtke, Thomas; Schmidt-Trucksäss, Arno; Brugger, Nicolas; Schäfer, Daniela; Saner, Hugo; Wilhelm, Matthias

    2014-01-01

    Marathon running has been linked with higher arterial stiffness. Blood pressure is a major contributor to pulse wave velocity (PWV). We examined indices of arterial stiffness with a blood pressure-independent method in marathon runners and ultra-endurance athletes. Male normotensive amateur runners were allocated to three groups according to former participation in competitions: group I (recreational athletes), group II (marathon runners) and group III (ultra-endurance athletes). Indices of arterial stiffness were measured with a non-invasive device (VaSera VS-1500N, Fukuda Denshi, Japan) to determine the cardio-ankle vascular index (CAVI, primary endpoint) and brachial-ankle PWV (baPWV). Lifetime training hours were calculated. Cumulative competitions were expressed as marathon equivalents. Linear regression analysis was performed to determine predictors for CAVI and baPWV. Measurements of arterial stiffness were performed in 51 subjects (mean age 44.6 ± 1.2 years): group I (n = 16), group II (n = 19) and group III (n = 16). No between-group differences existed in age, anthropometric characteristics and resting BP. CAVI and baPWV were comparable between all groups (P = 0.604 and P = 0.947, respectively). In linear regression analysis, age was the only independent predictor for CAVI (R(2) = 0.239, β = 0.455, P = 0.001). Systolic BP was significantly associated with baPWV (R(2) = 0.225, β = 0.403, P = 0.004). In middle-aged normotensive athletes marathon running and ultra-endurance sports had no negative impact on arterial stiffness.

  19. "Forty Acres and a Mule," With Interest: The Constitutionality of Black Capitalism, Benign School Quotas, and Other Statutory Racial Classifications

    ERIC Educational Resources Information Center

    Elden, Gary

    1969-01-01

    This article traces the growth and assesses contemporary patterns and consequences of, discrimination against the American Negro. The author contends that public policies designed to support black capitalism" and implement school racial quotas, are not only legal but necessary means toward achieving true equality of opportunity. (JH)

  20. Identification of methyl farnesoate from the hemolymph of insects

    USDA-ARS?s Scientific Manuscript database

    Juvenile hormones (JH) have been a focal point of study in insect endocrinology for more than 80 years and are implicated in regulation of more physiological and behavioral functions than any other insect hormone. Indeed, evidence has suggested that JHs are the only sesquiterpene hormone products s...

  1. Genetics Home Reference: Ellis-van Creveld syndrome

    MedlinePlus

    ... CREVELD SYNDROME Sources for This Page Baujat G, Le Merrer M. Ellis-van Creveld syndrome. Orphanet J Rare Dis. 2007 Jun ... CB, Kochilas L, Schimmenti LA, Moller JH. Ellis-van Creveld syndrome and congenital heart defects: presentation of an additional 32 cases. Pediatr Cardiol. 2011 Oct;32( ...

  2. It's Time to Teach Jurisprudence in High School

    ERIC Educational Resources Information Center

    Kahn, Stephen C.

    1975-01-01

    The concept of a "government of laws and not of men," representing the philosophy called legal positivism, is developed historically as it might be presented to a secondary school class. Some of the practical benefits from the teaching of the philosophy of law are also discussed. (JH)

  3. Heterotopic Ossification Following Combat-Related Trauma

    DTIC Science & Technology

    2010-01-01

    Nylen E, Brown TS, Rose MW, Stojadinovic A, Becker KL, McGuigan FX. Correlation of procalcitonin and cytokine expression with dehiscence of wartime...macular dystrophies . Arch Ophthalmol. 2003;121:967-72. 83. Schulmerich MV, Cole JH, Kreider JM, Esmonde-White F, Dooley KA, Goldstein SA, Morris MD

  4. Detection of Explosives on Surfaces Using UV Raman Spectroscopy: Effect of Substrate Color

    DTIC Science & Technology

    2017-10-01

    Jr, editors. Proc. SPIE 5794; Detection and Remediation Technologies for Mines and Minelike Targets X; 2005; Orlando, FL. Bellingham (WA): Society of...detection of 2,4-DNT in contact with sand particles. In: Harmon RS, Broach JT, Holloway JH Jr, editors. Proc. SPIE 6217; Detection and Remediation

  5. Genome-Wide Association Mapping for Intelligence in Military Working Dogs: Development of Advanced Classification Algorithm for Genome-Wide Single Nucleotide Polymorphism (SNP) Data Analysis

    DTIC Science & Technology

    2011-04-01

    critical. 5. REFERENCES Almasy, L, Blangero, J. (2009) “Human QTL linkage mapping.” Genetica 136:333-340. Amos, CI. (2007) “Successful...quantitative trait loci.” Genetica 136:237-243. Ward, JH, Hook, ME. “A Hierarchical Grouping Procedure Applied to a Problem of Grouping Profiles

  6. Performance of enhanced biological SBR process for aniline treatment by mycelial pellet as biomass carrier.

    PubMed

    Zhang, Si; Li, Ang; Cui, Di; Yang, Jixian; Ma, Fang

    2011-03-01

    Mycelial pellet of Aspergillus niger Y3 was used as a biomass carrier to immobilize the aniline-degrading bacterium, Acinetobacter calcoaceticus JH-9 and the mix culture of the COD rapid degradation bacteria. In order to investigate its removal effect on aniline and COD, the combined mycelial pellets were applied in the SBR. Comparison of the performances was conducted between another SBR inoculated with sole strain JH-9 and the above SBR. The results showed that the stable degradations of aniline and COD were observed in both reactors. In the SBR with combined mycelial pellet, the biological removal efficiency was about 0.9 mg aniline/(L·d). It was much higher than that in the activated sludge reactor. Meanwhile, the performances of the sedimentation velocity, liquid-solid phase separation and the effluent quality were better in the SBR. According to SEM images and PCR-DGGE analysis, the species immobilized on the biomass carrier were more predominant in this system. Copyright © 2010 Elsevier Ltd. All rights reserved.

  7. Juvenile hormone and colony conditions differentially influence cytochrome P450 gene expression in the termite Reticulitermes flavipes.

    PubMed

    Zhou, X; Song, C; Grzymala, T L; Oi, F M; Scharf, M E

    2006-12-01

    In lower termites, the worker caste is a totipotent immature stage that is capable of differentiating into other adult caste phenotypes. We investigated the diversity of family 4 cytochrome P450 (CYP4) genes in Reticulitermes flavipes workers, with the specific goal of identifying P450s potentially involved in regulating caste differentiation. Seven novel CYP4 genes were identified. Quantitative real-time PCR revealed the tissue distribution of expression for the seven CYP4s, as well as temporal expression changes in workers in association with a release from colony influences and during juvenile hormone (JH)-induced soldier caste differentiation. Several fat-body-related CYP4 genes were differentially expressed after JH treatment. Still other genes changed expression in association with removal from colony influences, suggesting that primer pheromones and/or other colony influences impact their expression. These findings add to a growing database of candidate termite caste-regulatory genes, and provide explicit evidence that colony factors influence termite gene expression.

  8. A whole genome screening and RNA interference identify a juvenile hormone esterase-like gene of the diamondback moth, Plutella xylostella.

    PubMed

    Gu, Xiaojun; Kumar, Sunil; Kim, Eunjin; Kim, Yonggyun

    2015-09-01

    Juvenile hormone (JH) plays a crucial role in preventing precocious metamorphosis and stimulating reproduction. Thus, its hemolymph titer should be under a tight control. As a negative controller, juvenile hormone esterase (JHE) performs a rapid breakdown of residual JH in the hemolymph during last instar to induce a larval-to-pupal metamorphosis. A whole genome of the diamondback moth (DBM), Plutella xylostella, has been annotated and proposed 11 JHE candidates. Sequence analysis using conserved motifs commonly found in other JHEs proposed a putative JHE (Px004817). Px004817 (64.61 kDa, pI=5.28) exhibited a characteristic JHE expression pattern by showing high peak at the early last instar, at which JHE enzyme activity was also at a maximal level. RNA interference of Px004817 reduced JHE activity and interrupted pupal development with a significant increase of larval period. This study identifies Px004817 as a JHE-like gene of P. xylostella. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Discovery of inhibitors that overcome the G1202R ALK Resistance Mutation

    PubMed Central

    Choi, Hwan Geun; Gao, Yang; Sim, Taebo; George, Rani; Jänne, Pasi A.; Gray, Nathanael S.

    2016-01-01

    The treatment of patients with advanced non-small cell lung cancer (NSCLC) harboring chromosomal rearrangements of anaplastic lymphoma kinase (ALK) has been revolutionized by the development of crizotinib, a small molecule inhibitor of ALK, ROS1, and MET. However, resistance to crizotinib inevitably develops through a variety of mechanisms leading to relapse both systemically and in the central nervous system (CNS). This has motivated the development of ‘second generation’ ALK inhibitors, including alectinib and ceritinib that overcome some of the mutations leading to resistance. However, most of the reported ALK inhibitors do not show inhibition of the G1202R mutant, which is one of the most common mutations. Herein, we report the development of a structural analogue of alectinib (JH-VIII-157-02) that is potent against the G1202R mutant as well as a variety of other frequently observed mutants. In addition, JH-VIII-157-02 is capable of penetrating the CNS of mice following oral dosing. PMID:26568289

  10. Influence of anionic substitution on the electrolyte electroreflectance study of band edge transitions in single crystal Cu2ZnSn(SxSe1-x)4 solid solutions

    NASA Astrophysics Data System (ADS)

    Levcenco, S.; Dumcenco, D.; Wang, Y. P.; Huang, Y. S.; Ho, C. H.; Arushanov, E.; Tezlevan, V.; Tiong, K. K.

    2012-06-01

    Single crystals of Cu2ZnSn(SxSe1-x)4 (CZTSSe) solid solutions were grown by chemical vapor transport technique using iodine trichloride as a transport agent. As confirmed by X-ray investigations, the as-grown CZTSSe solid solutions are single phase and crystallized in kesterite structure. The lattice parameters of CZTSSe were determined and the S contents of the obtained crystals were estimated by Vegard's law. The composition dependent band gaps of CZTSSe solid solutions were studied by electrolyte electroreflectance (EER) measurements at room temperature. From a detailed lineshape fit of the EER spectra, the band gaps of CZTSSe were determined accurately and were found to decrease almost linearly with the increase of Se content, which agreed well with the recent theoretical first-principle calculations by S. Chen, A. Walsh, J.H. Yang, X.G. Gong, L. Sun, P. X. Yang, J.H. Chu, S.H. Wei, Phys. Rev. B 83 (2011) 125201 (5pp).

  11. 75 FR 52756 - Government-Owned Inventions; Availability for Licensing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-27

    ... for evaluation of chemical species having unpaired electrons such as free radicals and transition.... Durnin J, Micheli J Jr, Eberly JH. Diffraction-free beams. Phys Rev Lett. 1987 Apr 13;58(15):1499-1501. 2... quantify pharmaco-kinetics and metabolic degradation kinetics of bioactive and redox sensitive free...

  12. North-South Migration of West Coast Low Pressure Centers

    ERIC Educational Resources Information Center

    McIntosh, C. Barron

    1974-01-01

    Monthly maps of low pressure centers are presented here to attempt a concrete representation that may help students to understand the seasonal change from dry months to wet months along the mid-latitude west coast as a seasonal north-south migration of factors controlling rain and drought. (Author/JH)

  13. Methyl farnesoate action, and morphogenetic signaling through the ligand binding pocket of the ortholog of the retinoid X receptor, in higher dipter

    USDA-ARS?s Scientific Manuscript database

    Most attention on metamorphic signaling by small terpenoids has focused action by juvenile hormone (JH) through bHLH-PAS proteins (e.g., MET and GCE), especially as that signaling axis intersects with ecdysteroid action through the receptor EcR. However, a long-standing series of endocrine and pharm...

  14. Examinations of Home Economics Textbooks for Sex Bias.

    ERIC Educational Resources Information Center

    Weis, Susan F.

    1979-01-01

    Four analyses were conducted on a sample of 100 randomly selected, secondary home economics textbooks published between 1964 and 1974. Results indicated that the contents presented sex bias in language usage, in pictures portraying male and female role environments, and in role behaviors and expectations emphasized. (Author/JH)

  15. Beyond the University to Mass Higher Education

    ERIC Educational Resources Information Center

    Maclure, Stuart

    1973-01-01

    "Life-long education," a spin-off mass secondary education, born of egalitarianism in education, reinforced by technological exigences, is a concept that needs close examination for it contains the seed of illiberality just as it offers a means of social engineering and a commitment to an open society. (JH)

  16. Integrated Thin Film DC Squids.

    DTIC Science & Technology

    1979-10-01

    3. G.B. Donaldson and H. Faghihi -Nejad, in Future Trends in Superconductive Electronis (B.S. Deaver, C.M. Falco, J.H. Harris and S.A. Wolf, Eds...STUDIED BY ELLIPSOMETRY G.B. Donaldcon, Department of Applied Physics, University cf Strathclyde, Glasgow, Scotland. H. Faghihi -Nejad*, Department of

  17. Good Things to Color and Draw. Cosas Buenas para Colorear y Dibujar.

    ERIC Educational Resources Information Center

    Florida Atlantic Univ., Boca Raton.

    Personal hygiene and self care skills are emphasized in a coloring book for bilingual students. This teaching aid is produced with Spanish and English captions or with only English captions. Opportunity for composing pictures by connecting dots and for freehand drawing as directed by captions is provided. (JH)

  18. DEVELOPMENTAL TOXICITY OF PERFLUOROOCATANE SULFONATE (PFOS) IN THE RAT AND MOUSE

    EPA Science Inventory

    1Lau, C., 1J.M. Rogers, 1R.G. Hanson*, 1B.D. Barbee*, 1M.G. Narotsky, 1J.E. Schmid* and 2J.H. Richards*. 1Reproductive Toxicology Division, and 2Environmental Toxicology Division, NHEERL, US EPA, Research Triangle Park, North Carolina. Developmental toxicity of Perfluorooctane ...

  19. Changes in power and force generation during coupled eccentric-concentric versus concentric muscle contraction with training and aging.

    PubMed

    Caserotti, Paolo; Aagaard, Per; Puggaard, Lis

    2008-05-01

    Age-related decline in maximal concentric muscle power is associated with frailty and functional impairments in the elderly. Compared to concentric contraction, mechanical muscle output is generally enhanced when muscles are rapidly pre-stretched (eccentric contraction), albeit less pronounced with increasing age. Exercise has been recommended to prevent loss of muscle power and function and recent guidelines indicate training program for increasing muscle power highly relevant for elderly subjects. This study examined the differences in muscle power, force and movement pattern during concentric-alone and coupled eccentric-concentric contraction and selected functional motor performances before and after 36-week multicomponent training including aerobic, strength, balance, flexibility and coordination components in elderly males. Vertical force, excursion, velocity, power and acceleration of the body center of mass were measured in two standardised vertical jumps (squatting jump, SQJ; countermovement jump, CMJ). Pre-stretch enhancement during CMJ did not improve performance [i.e., no enhanced maximal muscle power (Ppeak) and jump height (JH)] compared to concentric-alone muscle contraction (SQJ). Nevertheless, pre-stretch enhancement occurred as for similar SQJ and CMJ maximal performance, elderly people employed lower mechanical work, higher mean muscle power (Pmean), shorter concentric phase duration and shorter body center of mass displacement during CMJ. Post training, CMJ Ppeak, Pmean and JH increased in training group (P<0.05) while Ppeak and JH decreased in control group during the CMJ and SQJ (P<0.05). In conclusion, long-term training counteracted the age-related decline in muscle power and functional performance observed in the control subjects, while substantial gains in muscular performance were observed in the trained elderly.

  20. Effect of juvenile hormone and serotonin (5-HT) on mixis induction of the rotifer Brachionus plicatilis Muller.

    PubMed

    Gallardo; Hagiwara; Snell

    2000-09-05

    Juvenile hormone (JH) and serotonin (5-HT) were previously shown to enhance mictic (sexual) female production of the rotifer Brachionus plicatilis in batch cultures. To explore the basis of these effects, experiments were conducted on isolated individuals. JH treatment of maternal rotifers with 5 and 50 µgml(-1) (18.8 and 187.7 µM) resulted in significantly higher (P<0.05) mictic female production in the second (F(2)) and third (F(3)) generations. JH treatment was effective even at a lower food concentration of 7x10(5) cellsml(-1), but it was not effective when free ammonia was added at 2.4 and 3.1 µgml(-1). Mictic female production was not increased with exposure to 5-HT up to 50 µgml(-1) (129.1 µM) concentrations. When food level was reduced to 7x10(5) cellsml(-1), however, 5-HT-treated rotifers produced significantly (P<0.05) more mictic females than the control, particularly in F(3) generation. Mictic female production of 5-HT-treated rotifers did not differ from that of the control with or without free ammonia, but the intrinsic rate of natural increase (r) of 5-HT-treated rotifers at 3.1 µgml(-1) free ammonia was significantly higher than the control. These results show that juvenile hormone increases mictic female production under optimum and sub-optimum food levels, whereas 5-HT increases both mictic female production at low food level and population growth rate at high free ammonia concentrations. These compounds could be used to manage rotifer cultures and probe the mechanisms controlling the rotifer life cycle as it switches to mictic reproduction.

  1. RNAi-mediated knockdown of SPOOK reduces ecdysteroid titers and causes precocious metamorphosis in the desert locust Schistocerca gregaria.

    PubMed

    Sugahara, Ryohei; Tanaka, Seiji; Shiotsuki, Takahiro

    2017-09-01

    The Halloween gene SPOOK (SPO) is involved in the production of the active metabolite of ecdysteroid, 20-hydroxyecdysone (20E), in insects. A previous study showed that RNAi-mediated knockdown of SPO in Schistocerca gregaria last instar nymphs markedly reduced the hemolymph 20E titer, but did not affect metamorphosis. In the present study, the effects of SPO interference on development were re-examined in this locust. Injections of SPO double-stranded RNA (dsSPO) into nymphs at mid and late instars significantly delayed nymphal development and interfered with molting. The 20E levels of dsSPO-treated nymphs were generally low, with a delayed, small peak, suggesting that disturbance of the 20E levels caused the above developmental abnormalities. A small proportion of the dsSPO-injected nymphs metamorphosed precociously, producing adults and adultoids. Precocious adults were characterized by small body size, short wings with abbreviated venation, and normal reproductive activity. Fourth instar nymphs that precociously metamorphosed at the following instar exhibited temporal expression patterns of ecdysone-induced protein 93F and the juvenile hormone (JH) early-inducible gene Krüppel homolog 1 similar to those observed at the last instar in normal nymphs. Adultoids displayed mating behavior and adultoid females developed eggs, but never laid eggs. JH injection around the expected time of the 20E peak in the dsSPO-injected nymphs completely inhibited the appearance of adultoids, suggesting that appearance of adultoids might be due to a reduced titer of JH rather than of 20E. These results suggest that SPO plays an important role in controlling morphogenesis, metamorphosis, and reproduction in S. gregaria. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. A novel gene from the takeout family involved in termite trail-following behavior.

    PubMed

    Schwinghammer, Margaret A; Zhou, Xuguo; Kambhampati, Srinivas; Bennett, Gary W; Scharf, Michael E

    2011-03-15

    This study investigated physiological and behavioral functions of a novel gene identified from the termite Reticulitermes flavipes. The gene, named deviate, encodes an apparent ligand binding protein from the takeout-homologous family. Initial studies were conducted to investigate deviate mRNA expression among termite castes and body regions, and changes in response to light-dark conditions, starvation, temperature, and juvenile hormone (JH). Deviate has ubiquitous caste and tissue expression, including antennal expression. Consistent with characteristics of other takeout family members, deviate expression is responsive to photophase conditions (p<0.1), and feeding, temperature, and JH (p<0.05). Using RNA-interference (RNAi) techniques, short-interfering RNAs (siRNAs) homologous to the deviate gene were synthesized and injected into worker termites, which were then subjected to bioassays designed to (1) induce caste differentiation or (2) measure various behavioral aspects of foraging and trail following. No impacts on JH-dependent caste differentiation were observable. However, trail following accuracy was significantly reduced in termites that received deviate siRNA injections, and this pattern generally mirrored deviate mRNA attenuation and recovery after RNAi. In a subsequent distance foraging bioassay, deviate-silenced termites exhibited equal feeding levels to controls, suggesting the deviate gene is not linked to general vigor or the ability/motivation of termites to move and forage. These findings are among the first linking the expression of a termite gene with eusocial behavior; they illustrate the connection between deviate expression and trailing behavior, which is a key evolutionary adaptation vital to subterranean social insects such as termites and ants. Copyright © 2010 Elsevier B.V. All rights reserved.

  3. The Development of Adultoid Reproductives and Brachypterous Neotenic Reproductives From the Last Instar Nymphs in Reticulitermes labralis (Isoptera: Rhinotermitidae): A Comparative Study

    PubMed Central

    Su, Xiao Hong; Xue, Wei; Liu, He; Chen, Jiao Ling; Zhang, Xiao Jing; Xing, Lian Xi; Liu, Ming Hua

    2015-01-01

    Secondary reproductives develop primarily from nymphs. However, they have been rarely studied; in particular, the development of adultoid reproductives (AR) with floppy wings is still unclear. In this study, the change in juvenile hormone (JH) levels, vitellogenin gene expression, and oogenesis during the development of AR and brachypterous neotenic reproductives (BN) from the last instar nymphs of Reticulitermes labralis are investigated and compared. The results showed that the AR derived from the last instar nymphs by molting, and they were more similar to neotenic reproductives in morphology. In addition, the paired AR were not able to survive in the absence of workers. In R. labralis, the process of the last instar nymphs developing into AR and BN took an increase in JH level as a starting point. The JH level of the last instar nymphs molting into BN was approximately 1.5-fold higher than that of the AR. Additionally, The JHIII level of BN peaked on day 5, and that of AR peaked on day 10, which induced the onset of vitellogenesis in BN and AR, respectively. After molting, the vitellogenin gene expression levels of both BN and AR initially increased and then declined, and the expression levels in the BN were significantly higher than those in the AR. In addition, the oocytes of BN matured earlier than those of the AR, and the number of eggs laid by the BN was higher than the number laid by the AR. Our results demonstrate that, in R. labralis, the last instar nymphs can develop into AR, which are significantly different from BN in their development. PMID:26494776

  4. Relationships Between Concentric and Eccentric Strength and Countermovement Jump Performance in Resistance Trained Men.

    PubMed

    Bridgeman, Lee A; McGuigan, Michael R; Gill, Nicholas D; Dulson, Deborah K

    2018-01-01

    Bridgeman, LA, McGuigan, MR, Gill, ND, and Dulson, DK. Relationships between concentric and eccentric strength and countermovement jump performance in resistance trained men. J Strength Cond Res 32(1): 255-260, 2018-The purpose of this study was to investigate the relationships between concentric and eccentric peak force (PF) and countermovement jump (CMJ) performance in resistance trained men. Subjects were 12 men (mean ± SD; age: 25.4 ± 3.5 years; height: 177.2 ± 4.5 cm; mass: 84.0 ± 10.1 kg). The men were tested for concentric and eccentric PF using the Exerbotics squat device. Subjects then completed 3 CMJs to allow for the calculation of peak power (PP), peak ground reaction force, and jump height (JH). Correlations between the variables of interest were calculated using Pearson product-moment correlation coefficients. A large relationship was found between absolute concentric PF and absolute CMJ PP (r = 0.66, p ≤ 0.05). Absolute eccentric PF had a very large relationship with absolute CMJ PP and CMJ JH (r = 0.74, p < 0.01 and r = 0.74, p < 0.001, respectively). In addition, absolute eccentric PF was found to have a moderate relationship with relative CMJ PP (r = 0.58, p ≤ 0.05). Relative eccentric PF was had a very large relationship with relative CMJ PP and CMJ JH (r = 0.73, p < 0.001 and r = 0.79, p < 0.001, respectively). Based on these findings, strength and conditioning coaches and athletes who wish to enhance CMJ performance may wish to include exercises, which enhance lower-body eccentric strength within their training.

  5. Countermovement jump height: gender and sport-specific differences in the force-time variables.

    PubMed

    Laffaye, Guillaume; Wagner, Phillip P; Tombleson, Tom I L

    2014-04-01

    The goal of this study was to assess (a) the eccentric rate of force development, the concentric force, and selected time variables on vertical performance during countermovement jump, (b) the existence of gender differences in these variables, and (c) the sport-specific differences. The sample was composed of 189 males and 84 females, all elite athletes involved in college and professional sports (primarily football, basketball, baseball, and volleyball). The subjects performed a series of 6 countermovement jumps on a force plate (500 Hz). Average eccentric rate of force development (ECC-RFD), total time (TIME), eccentric time (ECC-T), Ratio between eccentric and total time (ECC-T:T) and average force (CON-F) were extracted from force-time curves and the vertical jumping performance, measured by impulse momentum. Results show that CON-F (r = 0.57; p < 0.001) and ECC-RFD (r = 0.52, p < 0.001) are strongly correlated with the jump height (JH), whereas the time variables are slightly and negatively correlated (r = -0.21-0.23, p < 0.01). Force variables differ between both sexes (p < 0.01), whereas time variables did not differ, showing a similar temporal structure. The best way to jump high is to increase CON-F and ECC-RFD thus minimizing the ECC-T. Principal component analysis (PCA) accounted for 76.8% of the JH variance and revealed that JH is predicted by a temporal and a force component. Furthermore, the PCA comparison made among athletes revealed sport-specific signatures: volleyball players revealed a temporal-prevailing profile, a weak-force with large ECC-T:T for basketball players and explosive and powerful profiles for football and baseball players.

  6. The four hexamerin genes in the honey bee: structure, molecular evolution and function deduced from expression patterns in queens, workers and drones

    PubMed Central

    2010-01-01

    Background Hexamerins are hemocyanin-derived proteins that have lost the ability to bind copper ions and transport oxygen; instead, they became storage proteins. The current study aimed to broaden our knowledge on the hexamerin genes found in the honey bee genome by exploring their structural characteristics, expression profiles, evolution, and functions in the life cycle of workers, drones and queens. Results The hexamerin genes of the honey bee (hex 70a, hex 70b, hex 70c and hex 110) diverge considerably in structure, so that the overall amino acid identity shared among their deduced protein subunits varies from 30 to 42%. Bioinformatics search for motifs in the respective upstream control regions (UCRs) revealed six overrepresented motifs including a potential binding site for Ultraspiracle (Usp), a target of juvenile hormone (JH). The expression of these genes was induced by topical application of JH on worker larvae. The four genes are highly transcribed by the larval fat body, although with significant differences in transcript levels, but only hex 110 and hex 70a are re-induced in the adult fat body in a caste- and sex-specific fashion, workers showing the highest expression. Transcripts for hex 110, hex 70a and hex70b were detected in developing ovaries and testes, and hex 110 was highly transcribed in the ovaries of egg-laying queens. A phylogenetic analysis revealed that HEX 110 is located at the most basal position among the holometabola hexamerins, and like HEX 70a and HEX 70c, it shares potential orthology relationship with hexamerins from other hymenopteran species. Conclusions Striking differences were found in the structure and developmental expression of the four hexamerin genes in the honey bee. The presence of a potential binding site for Usp in the respective 5' UCRs, and the results of experiments on JH level manipulation in vivo support the hypothesis of regulation by JH. Transcript levels and patterns in the fat body and gonads suggest that

  7. Effect of microbially mediated iron mineral transformation on temporal variation of arsenic in the Pleistocene aquifers of the central Yangtze River basin.

    PubMed

    Deng, Yamin; Zheng, Tianliang; Wang, Yanxin; Liu, Lun; Jiang, Hongchen; Ma, Teng

    2018-04-01

    Significant seasonal variation of groundwater arsenic (As) concentrations in shallow aquifers of the Jianghan Plain, central Yangtze River Basin has been reported recently, but the underlying mechanisms remain not well understood. To elaborate biogeochemical processes responsible for the observed As concentration variation, 42-day incubation experiments were done using sediment samples collected respectively from the depth of 26, 36 and 60m of the As-affected aquifer which were labeled respectively as JH26, JH36, JH60. Where JH denotes Jianghan Plain, and the number indicates the depth of the sediment sample. The results indicated that As could be mobilized from the sediments of 26m and 36m depth under the stimulation of exogenous organic carbon, with the maximum As release amount of 1.60 and 1.03mgkg -1 , respectively, while the sediments at 60m depth did not show As mobilization. The microbially mediated reductive dissolution of amorphous iron oxides and reduction of As(V) to As(III) could account for the observed As mobilization. The 16S rRNA high-throughput sequencing results indicated that the variation of microbial community correlated with the released As concentration (R=0.7, P<0.05) and the iron-reducing bacteria, including Pseudomonas, Clostridium and Geobacter, were the main drivers for the As mobilization from the sediments at 26m and 36m depth. The increase of arsC gene abundance (up to 1.4×10 5 copies g -1 ) during As release suggested that As reduction was mediated by the resistant reduction mechanism. By contrast, in the 60m sediments where the Fe and As release was absent, the iron-reducing bacteria accounted for a very minor proportion and sulfate-reducing bacteria were predominant in the microbial community. In addition, after 30days of incubation, the released As in the 26m sediments was immobilized via co-precipitation with or adsorption onto the Fe-sulfide mineral newly-formed by the bacterial sulfate reduction. These results are consistent

  8. Thinking about Faculty

    ERIC Educational Resources Information Center

    Light, Donald, Jr.

    1974-01-01

    Research and teaching do not integrate easily. There have been alternate models for higher education which did not rely on this combination. Restructuring the academic professions to conform with the reality of academic life can be accomplished by accepting an alternate model such as that of the good undergraduate teacher. (JH)

  9. Encoding Processes and Sex-Role Preferences

    ERIC Educational Resources Information Center

    Kail, Robert V., Jr.; Levine, Laura E.

    1976-01-01

    Seven and 10-year-olds were tested on memory and sex-role preference tasks. The memory task was the Wickens release from proactive inhibition paradigm in which short-term recall of words is tested on successive trials. Children selected favorite pictures from an array including masculine and feminine items. (JH)

  10. Faster vortex core switching with lower current density using three-nanocontact spin-polarized currents in a confined structure

    NASA Astrophysics Data System (ADS)

    Li, Hua-Nan; Hua, Zhong; Li, Dong-Fei

    2017-01-01

    Not Available Project supported by the China Postdoctoral Science Foundation (Grant No. 2013M541286), the Science and Technology Planning Project of Jilin Province, China (Grant Nos. 20140520109JH and 20150414003GH), and the “Twelfth Five year” Scientific and Technological Research Project of Department of Education of Jilin Province, China.

  11. Errors in Human Performance

    DTIC Science & Technology

    1980-08-15

    Instructional system, Including the works of Burton & -77 -;:-7 Final Report 3951 Brown (1979), Miller (1979), Goldstein (1979), and Stevens and Collins...rea-daAm i_=E1jJh. Providence, R.I.: Brown University Press, 1967. LaBerge , D., & Samuels, S. J. Toward a theory of automatic information processing

  12. Joint Staff Study, ICEBERG

    DTIC Science & Technology

    1944-12-21

    il UARINE OIVISION ltNMINE DIVISION 165- 170- «™., iig *™,^, . f .?ao-124-jh ICEBERG Appendix D Submarine. Operations 1. DISCUSSION. Commencing about...tender in the area, will continue in circulation and will b© inter—changeable at par with the . Supplementary Military Yen. Transaction in any other

  13. The Self, Political Socializatioon, and Education: Kenya as a Case Study

    ERIC Educational Resources Information Center

    Hopkins, Richard L.

    1973-01-01

    The political socialization of various African Kenyan leaders is used to illustrate the author's belief in H. G. Barnett's theory that the individual exerts an influence on the process of cultural change which is in opposition to Gabriel Almond's view that the individual is only a passive participant in society. (JH)

  14. Children's Interpretation of Evaluative Feedback: The Effect of Social Cues on Learned Helplessness

    ERIC Educational Resources Information Center

    Dweck, Carol S.

    1976-01-01

    Examines ways in which social cues, in conjunction with a child's history, influence the child's interpretation of and reaction to failure feedback in evaluative settings. It is suggested that the way in which a child reacts to another's behavior is largely dependent upon subtle but powerful social cues within situation. (JH)

  15. Do People Eat in Dining Halls as Often as They Say They Do

    DTIC Science & Technology

    1976-08-01

    Journal of Advertising Research , 1965...11, 1965, 11 Parfitt, J.H. A comparison of purchase recall with diary panel records. Journal of Advertising Research , 1967, September-, 31, 12See...purchase and use after six years. Journal of Advertising Research , 1965, September, 16. Haley, R.I., & Gatty, R. Monitor your market continously.

  16. Modelling Conceptual Development: A computational Account of Conservation Learning

    DTIC Science & Technology

    1990-02-28

    6 year-old age range are unable to obtain specific quantitative values for larger sets of objects e.g. (Gelman & Gallistel , 1978). 3. Children who...Piagetian concepts. In J.H. Flavell & E.M. Markman (Eds.), Handbook of Child Psychology(Fourth ed.). New York: Wiley. Gelman, R. & Gallistel , R.C. (1978

  17. OVERVIEW.

    ERIC Educational Resources Information Center

    ROSENBERG, SHELDON

    THIS OVERVIEW CHAPTER INTRODUCES THE FORTHCOMING "DEVELOPMENTS IN APPLIED PSYCHOLINGUISTICS RESEARCH," S. ROSENBERG AND J.H. KOPLIN, EDITORS, WHICH WILL BE PUBLISHED IN 1968 BY MACMILLAN COMPANY. IT WAS DESIGNED TO SERVE AN INTEGRATIVE FUNCTION--TO IDENTIFY SOME OF THE MAJOR IDEAS AND CONCERNS OF THE CONTRIBUTORS, TO IDENTIFY SOME OF THEIR…

  18. Pointing to Parallels in Ability-Related Differences in the Use of Metacognition in Academic and Psychomotor Tasks

    ERIC Educational Resources Information Center

    Martini, Rose; Shore, Bruce M.

    2008-01-01

    This paper offers a brief review of the use of metacognition by proficient and poor performers in academic and psychomotor tasks as well as highlights the parallels and provides directions for future research. Metacognition is knowledge about one's own cognitive processes [Flavell, J.H. (1979). "Metacognition and cognitive monitoring: A new area…

  19. Non-Linear Vibrations, Stability, and Dynamics of Structures and Mechanisms

    DTIC Science & Technology

    1989-08-01

    account. This kinematic hypothesis has been employed by many investigators ( Antman and Jordan 1975, Reissner 1973, 1981, and Simo and Vu-Quoc 1986). In...the preparation of this manuscript is thankfully acknowledged. 1. S.S. Antman and K.B. Jordan, Proc. R. Soc. Edinb. 73A (5), 85-105 (1975). 2. J.H

  20. Fashions in Education

    ERIC Educational Resources Information Center

    Lawrence, Frances

    1973-01-01

    In this historical view of the 19th century British initiatives to learn about Continental education methods and problems, the author challenges present-day educationists to reestablish that initiative and enthusiasm, cut off by World War I, so that today more than strictly comparative data can be shared in European education. (JH)

  1. Sex Discrimination in Professional Employment: A Case Study.

    ERIC Educational Resources Information Center

    Osterman, Paul

    1979-01-01

    A study analyzed sex discrimination with data on over 700 professional employees in a metropolitan publishing firm. It was found that the sex differential in earnings within clusters of similar jobs was much greater if marriage and children variables were excluded: men received a large "payoff" from being married and having children. (JH)

  2. Education toward a More Economic Life Style.

    ERIC Educational Resources Information Center

    Paynton, Naomi

    1979-01-01

    A community-based project was carried out in two low-income, high density areas in Tel Aviv, Israel, to help mothers of large families gain more from the existing family budget. Areas covered included clothing, nutrition, household maintenance, and savings and insurance. Behavioral changes were greatest in the area of clothing. (Author/JH)

  3. Student-Prepared Games as a Teaching Strategy

    ERIC Educational Resources Information Center

    Brisk, William J.

    1974-01-01

    A course on game preparation attended by students from different disciplines involved an exposure to various types of games, leading up to individual and group development of games. Two of the games developed, Senate and Disarmament, described in the article, exemplify the benefit in hard deductive reasoning required by game preparation. (JH)

  4. Genomic analysis of the interaction between pesticide exposure and nutrition in honey bees (Apis mellifera).

    PubMed

    Schmehl, Daniel R; Teal, Peter E A; Frazier, James L; Grozinger, Christina M

    2014-12-01

    Populations of pollinators are in decline worldwide. These declines are best documented in honey bees and are due to a combination of stressors. In particular, pesticides have been linked to decreased longevity and performance in honey bees; however, the molecular and physiological pathways mediating sensitivity and resistance to pesticides are not well characterized. We explored the impact of coumaphos and fluvalinate, the two most abundant and frequently detected pesticides in the hive, on genome-wide gene expression patterns of honey bee workers. We found significant changes in 1118 transcripts, including genes involved in detoxification, behavioral maturation, immunity, and nutrition. Since behavioral maturation is regulated by juvenile hormone III (JH), we examined effects of these miticides on hormone titers; while JH titers were unaffected, titers of methyl farnesoate (MF), the precursor to JH, were decreased. We further explored the association between nutrition- and pesticide-regulated gene expression patterns and demonstrated that bees fed a pollen-based diet exhibit reduced sensitivity to a third pesticide, chlorpyrifos. Finally, we demonstrated that expression levels of several of the putative pesticide detoxification genes identified in our study and previous studies are also upregulated in response to pollen feeding, suggesting that these pesticides and components in pollen modulate similar molecular response pathways. Our results demonstrate that pesticide exposure can substantially impact expression of genes involved in several core physiological pathways in honey bee workers. Additionally, there is substantial overlap in responses to pesticides and pollen-containing diets at the transcriptional level, and subsequent analyses demonstrated that pollen-based diets reduce workers' pesticide sensitivity. Thus, providing honey bees and other pollinators with high quality nutrition may improve resistance to pesticides. Copyright © 2014 Elsevier Ltd. All

  5. Dissection of Rovibronic Structure by Polarization-Resolved Two-Color Resonant Four-Wave Mixing Spectroscopy

    NASA Astrophysics Data System (ADS)

    Murdock, Daniel; Burns, Lori A.; Vaccaro, Patrick H.

    2009-08-01

    A synergistic theoretical and experimental investigation of stimulated emission pumping (SEP) as implemented in the coherent framework of two-color resonant four-wave mixing (TC-RFWM) spectroscopy is presented, with special emphasis directed toward the identification of polarization geometries that can distinguish spectral features according to their attendant changes in rotational quantum numbers. A vector-recoupling formalism built upon a perturbative treatment of matter-field interactions and a state-multipole expansion of the density operator allowed the weak-field signal intensity to be cast in terms of a TC-RFWM response tensor, RQ(K)(ɛ4*ɛ3ɛ2*ɛ1;Jg,Je,Jh,Jf), which separates the transverse characteristics of the incident and generated electromagnetic waves (ɛ4*ɛ3ɛ2*ɛ1) from the angular momentum properties of the PUMP and DUMP resonances (Jg,Je,Jh,Jf). For an isolated SEP process induced in an isotropic medium, the criteria needed to discriminate against subsets of rovibronic structure were encoded in the roots of a single tensor element, R0(0)(ɛ4*ɛ3ɛ2*ɛ1;Jg,Je,Jh,Je). By assuming all optical fields to be polarized linearly and invoking the limit of high quantum numbers, specific angles of polarization for the detected signal field were found to suppress DUMP resonances selectively according to the nature of their rotational branch and the rotational branch of the meshing PUMP line. These predictions were corroborated by performing SEP measurements on the ground electronic potential energy surface of tropolone in two distinct regimes of vibrational excitation, with the near-ultraviolet Ã1B2-X˜1A1 (π* ← π) absorption system affording the requisite PUMP and DUMP transitions.

  6. Dissection of rovibronic structure by polarization-resolved two-color resonant four-wave mixing spectroscopy.

    PubMed

    Murdock, Daniel; Burns, Lori A; Vaccaro, Patrick H

    2009-11-26

    A synergistic theoretical and experimental investigation of stimulated emission pumping (SEP) as implemented in the coherent framework of two-color resonant four-wave mixing (TC-RFWM) spectroscopy is presented, with special emphasis directed toward the identification of polarization geometries that can distinguish spectral features according to their attendant changes in rotational quantum numbers. A vector-recoupling formalism built upon a perturbative treatment of matter-field interactions and a state-multipole expansion of the density operator allowed the weak-field signal intensity to be cast in terms of a TC-RFWM response tensor, RQ(K)(epsilon4*epsilon3epsilon2*epsilon1;Jg,Je,Jh,Jf), which separates the transverse characteristics of the incident and generated electromagnetic waves (epsilon4*epsilon3epsilon2*epsilon1) from the angular momentum properties of the PUMP and DUMP resonances (Jg,Je,Jh,Jf). For an isolated SEP process induced in an isotropic medium, the criteria needed to discriminate against subsets of rovibronic structure were encoded in the roots of a single tensor element, R0(0)(epsilon4*epsilon3epsilon2*epsilon1;Jg,Je,Jh,Je). By assuming all optical fields to be polarized linearly and invoking the limit of high quantum numbers, specific angles of polarization for the detected signal field were found to suppress DUMP resonances selectively according to the nature of their rotational branch and the rotational branch of the meshing PUMP line. These predictions were corroborated by performing SEP measurements on the ground electronic potential energy surface of tropolone in two distinct regimes of vibrational excitation, with the near-ultraviolet 1B2-1A1 (pi*<--pi) absorption system affording the requisite PUMP and DUMP transitions.

  7. Elastic recoil detection analysis for the determination of hydrogen concentration profiles in switchable mirrors

    NASA Astrophysics Data System (ADS)

    Huisman, M. C.; van der Molen, S. J.; Vis, R. D.

    1999-10-01

    Switchable mirrors [J.N. Huiberts, R. Griessen, J.H. Rector, R.J. Wijngaarden, J.P. Dekker, D.G. de Groot, N.J. Koeman, Nature 380 (1996) 231; J.N Huiberts, J.H. Rector, R.J. Wijngaarden, S. Jetten, D. de Groot, B. Dam, N.J.. Koeman, R. Griessen, B. Hjörvarsson, S Olafsson, Y.S. Cho, J. Alloys and Compounds 239 (1996) 158; F.J.A. den Broeder, S.J. van der Molen, M. Kremers, J. N. Huiberts, D.G. Nagengast, A.T.M. van Gogh, W.H. Huisman, N. J. Koeman, B. Dam, J.H. Rector, S. Plota, M. Haaksma, R.M.N. Hanzen, R.M. Jungblut, P.A. Duine, R. Griessen, Nature 394 (1998) 656] made of thin films of Y, La or rare-earth (RE) metals exhibit spectacular changes in their optical and electrical properties upon hydrogen loading. The study of these materials has indicated that the occurring phenomena are highly sensitive to the actual hydrogen concentration in these materials. In this paper elastic recoil detection analysis (ERDA) is used as a tool to measure hydrogen concentrations on a micrometer scale. The measurements have been performed using a 4He 2+ ion beam from a 1.7 MV Pelletron accelerator. The ion beam can be focused routinely to a spot size of approximately 10 μm 2. The experimental set-up enables the simultaneous measurement of Rutherford backscattering spectrometry (RBS) as well as particle induced X-ray emission (PIXE) spectra, which provide complementary information. The results of ERDA measurements on laterally diffused YH x (0< x<3) samples with a qualitatively known hydrogen concentration profile are presented and discussed. The calibration of the microbeam set-up and possible improvement of the measurement technique are described.

  8. The 3D-HST Survey: Hubble Space Telescope WFC3/G141 Grism Spectra, Redshifts, and Emission Line Measurements for ~ 100,000 Galaxies

    NASA Astrophysics Data System (ADS)

    Momcheva, Ivelina G.; Brammer, Gabriel B.; van Dokkum, Pieter G.; Skelton, Rosalind E.; Whitaker, Katherine E.; Nelson, Erica J.; Fumagalli, Mattia; Maseda, Michael V.; Leja, Joel; Franx, Marijn; Rix, Hans-Walter; Bezanson, Rachel; Da Cunha, Elisabete; Dickey, Claire; Förster Schreiber, Natascha M.; Illingworth, Garth; Kriek, Mariska; Labbé, Ivo; Ulf Lange, Johannes; Lundgren, Britt F.; Magee, Daniel; Marchesini, Danilo; Oesch, Pascal; Pacifici, Camilla; Patel, Shannon G.; Price, Sedona; Tal, Tomer; Wake, David A.; van der Wel, Arjen; Wuyts, Stijn

    2016-08-01

    We present reduced data and data products from the 3D-HST survey, a 248-orbit HST Treasury program. The survey obtained WFC3 G141 grism spectroscopy in four of the five CANDELS fields: AEGIS, COSMOS, GOODS-S, and UDS, along with WFC3 H 140 imaging, parallel ACS G800L spectroscopy, and parallel I 814 imaging. In a previous paper, we presented photometric catalogs in these four fields and in GOODS-N, the fifth CANDELS field. Here we describe and present the WFC3 G141 spectroscopic data, again augmented with data from GO-1600 in GOODS-N (PI: B. Weiner). We developed software to automatically and optimally extract interlaced two-dimensional (2D) and one-dimensional (1D) spectra for all objects in the Skelton et al. (2014) photometric catalogs. The 2D spectra and the multi-band photometry were fit simultaneously to determine redshifts and emission line strengths, taking the morphology of the galaxies explicitly into account. The resulting catalog has redshifts and line strengths (where available) for 22,548 unique objects down to {{JH}}{IR}≤slant 24 (79,609 unique objects down to {{JH}}{IR}≤slant 26). Of these, 5459 galaxies are at z\\gt 1.5 and 9621 are at 0.7\\lt z\\lt 1.5, where Hα falls in the G141 wavelength coverage. The typical redshift error for {{JH}}{IR}≤slant 24 galaxies is {σ }z≈ 0.003× (1+z), I.e., one native WFC3 pixel. The 3σ limit for emission line fluxes of point sources is 2.1× {10}-17 erg s-1 cm-2. All 2D and 1D spectra, as well as redshifts, line fluxes, and other derived parameters, are publicly available.18

  9. Precocious Metamorphosis in the Juvenile Hormone–Deficient Mutant of the Silkworm, Bombyx mori

    PubMed Central

    Daimon, Takaaki; Kozaki, Toshinori; Niwa, Ryusuke; Kobayashi, Isao; Furuta, Kenjiro; Namiki, Toshiki; Uchino, Keiro; Banno, Yutaka; Katsuma, Susumu; Tamura, Toshiki; Mita, Kazuei; Sezutsu, Hideki; Nakayama, Masayoshi; Itoyama, Kyo; Shimada, Toru; Shinoda, Tetsuro

    2012-01-01

    Insect molting and metamorphosis are intricately governed by two hormones, ecdysteroids and juvenile hormones (JHs). JHs prevent precocious metamorphosis and allow the larva to undergo multiple rounds of molting until it attains the proper size for metamorphosis. In the silkworm, Bombyx mori, several “moltinism” mutations have been identified that exhibit variations in the number of larval molts; however, none of them have been characterized molecularly. Here we report the identification and characterization of the gene responsible for the dimolting (mod) mutant that undergoes precocious metamorphosis with fewer larval–larval molts. We show that the mod mutation results in complete loss of JHs in the larval hemolymph and that the mutant phenotype can be rescued by topical application of a JH analog. We performed positional cloning of mod and found a null mutation in the cytochrome P450 gene CYP15C1 in the mod allele. We also demonstrated that CYP15C1 is specifically expressed in the corpus allatum, an endocrine organ that synthesizes and secretes JHs. Furthermore, a biochemical experiment showed that CYP15C1 epoxidizes farnesoic acid to JH acid in a highly stereospecific manner. Precocious metamorphosis of mod larvae was rescued when the wild-type allele of CYP15C1 was expressed in transgenic mod larvae using the GAL4/UAS system. Our data therefore reveal that CYP15C1 is the gene responsible for the mod mutation and is essential for JH biosynthesis. Remarkably, precocious larval–pupal transition in mod larvae does not occur in the first or second instar, suggesting that authentic epoxidized JHs are not essential in very young larvae of B. mori. Our identification of a JH–deficient mutant in this model insect will lead to a greater understanding of the molecular basis of the hormonal control of development and metamorphosis. PMID:22412378

  10. Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex.

    PubMed

    Toyota, Kenji; Miyakawa, Hitoshi; Hiruta, Chizue; Furuta, Kenjiro; Ogino, Yukiko; Shinoda, Tetsuro; Tatarazako, Norihisa; Miyagawa, Shinichi; Shaw, Joseph R; Iguchi, Taisen

    2015-09-01

    Sex-determination systems can be divided into two groups: genotypic sex determination (GSD) and environmental sex determination (ESD). ESD is an adaptive life-history strategy that allows control of sex in response to environmental cues in order to optimize fitness. However, the molecular basis of ESD remains largely unknown. The micro crustacean Daphnia pulex exhibits ESD in response to various external stimuli. Although methyl farnesoate (MF: putative juvenile hormone, JH, in daphnids) has been reported to induce male production in daphnids, the role of MF as a sex-determining factor remains elusive due to the lack of a suitable model system for its study. Here, we establish such a system for ESD studies in D. pulex. The WTN6 strain switches from producing females to producing males in response to the shortened day condition, while the MFP strain only produces females, irrespective of day-length. To clarify whether MF has a novel physiological role as a sex-determining factor in D. pulex, we demonstrate that a MF/JH biosynthesis inhibitor suppressed male production in WTN6 strain reared under the male-inducible condition, shortened day-length. Moreover, we show that juvenile hormone acid O-methyltransferase (JHAMT), a critical enzyme of MF/JH biosynthesis, displays MF-generating activity by catalyzing farnesoic acid. Expression of the JHAMT gene increased significantly just before the MF-sensitive period for male production in the WTN6 strain, but not in the MFP strain, when maintained under male-inducible conditions. These results suggest that MF synthesis regulated by JHAMT is necessary for male offspring production in D. pulex. Our findings provide novel insights into the genetic underpinnings of ESD and they begin to shed light on the physiological function of MF as a male-fate determiner in D. pulex. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Obesogens beyond Vertebrates: Lipid Perturbation by Tributyltin in the Crustacean Daphnia magna

    PubMed Central

    Jordão, Rita; Casas, Josefina; Fabrias, Gemma; Campos, Bruno; Piña, Benjamín; Lemos, Marco F.L.; Soares, Amadeu M.V.M.; Tauler, Romà

    2015-01-01

    Background The analysis of obesogenic effects in invertebrates is limited by our poor knowledge of the regulatory pathways of lipid metabolism. Recent data from the crustacean Daphnia magna points to three signaling hormonal pathways related to the molting and reproductive cycles [retinoic X receptor (RXR), juvenile hormone (JH), and ecdysone] as putative targets for exogenous obesogens. Objective The present study addresses the disruptive effects of the model obesogen tributyltin (TBT) on the lipid homeostasis in Daphnia during the molting and reproductive cycle, its genetic control, and health consequences of its disruption. Methods D. magna individuals were exposed to low and high levels of TBT. Reproductive effects were assessed by Life History analysis methods. Quantitative and qualitative changes in lipid droplets during molting and the reproductive cycle were studied using Nile red staining. Lipid composition and dynamics were analyzed by ultra-performance liquid chromatography coupled to a time-of-flight mass spectrometer. Relative abundances of mRNA from different genes related to RXR, ecdysone, and JH signaling pathways were studied by qRT-PCR. Results and Conclusions TBT disrupted the dynamics of neutral lipids, impairing the transfer of triacylglycerols to eggs and hence promoting their accumulation in adult individuals. TBT’s disruptive effects translated into a lower fitness for offspring and adults. Co-regulation of gene transcripts suggests that TBT activates the ecdysone, JH, and RXR receptor signaling pathways, presumably through the already proposed interaction with RXR. These findings indicate the presence of obesogenic effects in a nonvertebrate species. Citation Jordão R, Casas J, Fabrias G, Campos B, Piña B, Lemos MF, Soares AM, Tauler R, Barata C. 2015. Obesogens beyond vertebrates: lipid perturbation by tributyltin in the crustacean Daphnia magna. Environ Health Perspect 123:813–819; http://dx.doi.org/10.1289/ehp.1409163 PMID

  12. Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris.

    PubMed

    Jedlička, Pavel; Ernst, Ulrich R; Votavová, Alena; Hanus, Robert; Valterová, Irena

    2016-01-01

    Understanding the social evolution leading to insect eusociality requires, among other, a detailed insight into endocrine regulatory mechanisms that have been co-opted from solitary ancestors to play new roles in the complex life histories of eusocial species. Bumblebees represent well-suited models of a relatively primitive social organization standing on the mid-way to highly advanced eusociality and their queens undergo both, a solitary and a social phase, separated by winter diapause. In the present paper, we characterize the gene expression levels of major endocrine regulatory pathways across tissues, sexes, and life-stages of the buff-tailed bumblebee, Bombus terrestris , with special emphasis on critical stages of the queen's transition from solitary to social life. We focused on fundamental genes of three pathways: (1) Forkhead box protein O and insulin/insulin-like signaling, (2) Juvenile hormone (JH) signaling, and (3) Adipokinetic hormone signaling. Virgin queens were distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and JH signaling, indicated by low expression of methyl farnesoate epoxidase ( MFE ) and transcription factor Krüppel homolog 1 ( Kr-h1 ). Diapausing queens showed the expected downregulation of JH signaling in terms of low MFE and vitellogenin ( Vg ) expressions, but an unexpectedly high expression of Kr-h1 . By contrast, reproducing queens revealed an upregulation of MFE and Vg together with insulin signaling. Surprisingly, the insulin growth factor 1 (IGF-1) turned out to be a queen-specific hormone. Workers exhibited an expression pattern of MFE and Vg similar to that of reproducing queens. Males were characterized by high Kr-h1 expression and low Vg level. The tissue comparison unveiled an unexpected resemblance between the fat body and hypopharyngeal glands across all investigated genes, sexes, and life stages.

  13. Transformation and Precipitation of Toxic Metals by ’Pseudomonas maltophilia’

    DTIC Science & Technology

    1991-05-31

    site" R.C. Blake, D.M. Choate, S.H. Bardhan , N.H. Revis, L.L. Barton, and T.G. Zocco; submitted to Environmental Toxicology and Chemistry 4 (iii) In...D.M Choate, S.H. Bardhan , N.H. Revis, and J.H. Jackson; to be submitted to the Journal of Biological Chemistry D. PROFESSIONAL PERSONNEL (i

  14. Signaling Pathways that Mediate Neurotoxin-Induced Death of Dopamine Neurons

    DTIC Science & Technology

    2008-11-01

    lessons from the analysis of mutant mice. J. Cell Biol. 157, 441–453. Reimertz C., Kogel D., Rami A., Chittenden T. and Prehn J. H. (2003) Gene expression...Bauerbach E, Poppe M, Krieglstein J, Prehn JH (2002) p75 neurotrophin receptor is required for constitutive and NGF-induced survival signalling in PC12

  15. Visitors from the Halo: 11 Gyr Old White Dwarfs in the Solar Neighborhood

    DTIC Science & Technology

    2010-05-20

    nearby Two Micron All Sky Survey ( 2MASS ) stars to calibrate the photometry. The optical and near-infrared photometry of our targets, as well as proper...motions, are presented in Table 1. The optical photometry is in the AB system and the JH photometry is in the 2MASS (Vega) system. We use the

  16. Lotus alianus, a new species from Cabo Verde and nomeenclatural notes on Lotus section Pedrosia (Fabaceae)

    USDA-ARS?s Scientific Manuscript database

    Lotus alianus J.H. Kirkbr., sp. nov., is described and illustrated. It is a rare endemic species from the Republic of Cape Verde, and is found in dry habitats on just two islands, Ilhas de Santo Antao and Sao Vicente. In addition, two species names are synonymized with L. creticus L., and a lectotyp...

  17. Cyclic stress induced phase transformation in super-bainitic microstructure

    NASA Astrophysics Data System (ADS)

    Xiu, Wencui; Han, Ying; Liu, Cheng; Wu, Hua; Liu, Yunxu

    2017-03-01

    Not Available Project supported by the National Natural Science Foundation of China (Grant Nos. 51171030 and 51604034), the Scientific and Technological Planning Project of Jilin Province, China (Grant No. 20150520030JH), and the Scientific and Technological Research Fund of Jilin Provincial Education Department during the Twelfth Five-year Plan Period, China (Grant No. 2015-95).

  18. A Laboratory Experiment on Oil Weathering under Arctic Conditions.

    DTIC Science & Technology

    1982-09-01

    water ( Fazal and Milgram, 1977), and both on the surface and underneath a smooth solid ice sheet (Cox et al., 1980). In addition, Free et al. (1981...Covered Waters of Buzzards Bay," NOAA OCSEAP Report, Boulder, Colorado, June 1977. 3. Fazal , R.A. and J.H. Milgram, "The Effects of Surface Phenomena

  19. Targeting Paclitaxel-Loaded Nanoparticles to Ovarian Cancer

    DTIC Science & Technology

    2011-05-01

    with each other causes the polymer to collapse to form a nanoparticle of ~20 nm in aqueous solutions as determined by dynamic light scattering (2, 8...molecular target in tumor cells and tumor stroma. Cancer Res. 2008;68:7210-8. 19. von Maltzahn G, Ren Y, Park JH, Min DH, Kotamraju VR, Jayakumar J, et

  20. Genetics Home Reference: Greig cephalopolysyndactyly syndrome

    MedlinePlus

    ... Bocian M, Bodurtha JN, Cox GF, Curry CJ, Day R, Donnai D, Field M, Fujiwara I, Gabbett M, Gal M, Graham JM, Hedera P, Hennekam RC, Hersh JH, Hopkin RJ, Kayserili H, Kidd AM, Kimonis V, Lin AE, Lynch SA, Maisenbacher M, Mansour S, McGaughran J, Mehta L, Murphy H, Raygada M, Robin NH, ...

  1. Genetics Home Reference: Pallister-Hall syndrome

    MedlinePlus

    ... Bocian M, Bodurtha JN, Cox GF, Curry CJ, Day R, Donnai D, Field M, Fujiwara I, Gabbett M, Gal M, Graham JM, Hedera P, Hennekam RC, Hersh JH, Hopkin RJ, Kayserili H, Kidd AM, Kimonis V, Lin AE, Lynch SA, Maisenbacher M, Mansour S, McGaughran J, Mehta L, Murphy H, Raygada M, Robin NH, ...

  2. Archeological and Historic Cultural Resources Inventory for a Proposed Flood Control Project at Devils Lake, Ramsey County, North Dakota.

    DTIC Science & Technology

    1983-10-01

    Chautauqua Association signed a contract with J.H. McCulloch to secure a right-of- way for the Chautauqua Railway. McCulloch received a 30 year franchise in...excavation at Bakery of U.S. military fort Palo Duro St. Park, TX; Test excavation at historic dugout Sea Rim State Park, TX; Archaeological survey San

  3. Endocrine remodelling of the adult intestine sustains reproduction in Drosophila

    PubMed Central

    Reiff, Tobias; Jacobson, Jake; Cognigni, Paola; Antonello, Zeus; Ballesta, Esther; Tan, Kah Junn; Yew, Joanne Y; Dominguez, Maria; Miguel-Aliaga, Irene

    2015-01-01

    The production of offspring is energetically costly and relies on incompletely understood mechanisms that generate a positive energy balance. In mothers of many species, changes in key energy-associated internal organs are common yet poorly characterised functionally and mechanistically. In this study, we show that, in adult Drosophila females, the midgut is dramatically remodelled to enhance reproductive output. In contrast to extant models, organ remodelling does not occur in response to increased nutrient intake and/or offspring demands, but rather precedes them. With spatially and temporally directed manipulations, we identify juvenile hormone (JH) as an anticipatory endocrine signal released after mating. Acting through intestinal bHLH-PAS domain proteins Methoprene-tolerant (Met) and Germ cell-expressed (Gce), JH signals directly to intestinal progenitors to yield a larger organ, and adjusts gene expression and sterol regulatory element-binding protein (SREBP) activity in enterocytes to support increased lipid metabolism. Our findings identify a metabolically significant paradigm of adult somatic organ remodelling linking hormonal signals, epithelial plasticity, and reproductive output. DOI: http://dx.doi.org/10.7554/eLife.06930.001 PMID:26216039

  4. Evaluation of tranche in securitization and long-range Ising model

    NASA Astrophysics Data System (ADS)

    Kitsukawa, K.; Mori, S.; Hisakado, M.

    2006-08-01

    This econophysics work studies the long-range Ising model of a finite system with N spins and the exchange interaction J/N and the external field H as a model for homogeneous credit portfolio of assets with default probability Pd and default correlation ρd. Based on the discussion on the (J,H) phase diagram, we develop a perturbative calculation method for the model and obtain explicit expressions for Pd,ρd and the normalization factor Z in terms of the model parameters N and J,H. The effect of the default correlation ρd on the probabilities P(Nd,ρd) for Nd defaults and on the cumulative distribution function D(i,ρd) are discussed. The latter means the average loss rate of the“tranche” (layered structure) of the securities (e.g. CDO), which are synthesized from a pool of many assets. We show that the expected loss rate of the subordinated tranche decreases with ρd and that of the senior tranche increases linearly, which are important in their pricing and ratings.

  5. Lepidopteran HMG-CoA reductase is a potential selective target for pest control

    PubMed Central

    Li, Yuan-mei; Huang, Juan; Tobe, Stephen S.

    2017-01-01

    As a consequence of the negative impacts on the environment of some insecticides, discovery of eco-friendly insecticides and target has received global attention in recent years. Sequence alignment and structural comparison of the rate-limiting enzyme HMG-CoA reductase (HMGR) revealed differences between lepidopteran pests and other organisms, which suggested insect HMGR could be a selective insecticide target candidate. Inhibition of JH biosynthesis in vitro confirmed that HMGR inhibitors showed a potent lethal effect on the lepidopteran pest Manduca sexta, whereas there was little effect on JH biosynthesis in Apis mellifera and Diploptera punctata. The pest control application of these inhibitors demonstrated that they can be insecticide candidates with potent ovicidal activity, larvicidal activity and insect growth regulatory effects. The present study has validated that Lepidopteran HMGR can be a potent selective insecticide target, and the HMGR inhibitors (especially type II statins) could be selective insecticide candidates and lead compounds. Furthermore, we demonstrated that sequence alignment, homology modeling and structural comparison may be useful for determining potential enzymes or receptors which can be eco-friendly pesticide  targets. PMID:28133568

  6. Cloning of the cDNA encoding Scg-SPRP, an unusual Ser-protease-related protein from vitellogenic female desert locusts (Schistocerca gregaria).

    PubMed

    Chiou, S J; Vanden Broeck, J; Janssen, I; Borovsky, D; Vandenbussche, F; Simonet, G; De Loof, A

    1998-10-01

    The cDNA coding for a Ser-protease-related protein (Scg-SPRP) was cloned from desert locust (Schistocerca gregaria) midgut. The derived amino acid sequence consists of 260 residues and shows strong sequence similarity to insect trypsin-like molecules. It is, however, likely that Scg-SPRP is not a proteolytically active enzyme and that it plays another physiologically relevant role, since two out of three residues which are indispensable for catalytic activity of Ser-proteases are replaced. Northern analysis revealed that the Scg-SPRP gene is expressed in midgut tissue and that this expression is strongly induced in adult female locusts. Moreover, the occurrence of the transcript (1.2 kb) fluctuates during the molting cycle and during the female reproductive cycle. Juvenile hormone (JH III) dependence of transcription was investigated by chemical allatectomy (precocene I) of adult females. This resulted in inhibition of vitellogenesis and in disappearance of the Scg-SPRP transcript. Expression of Scg-SPRP in precocene-treated locusts could be reinduced by additional treatment with JH III or with 20-OH-ecdysone.

  7. Structure-Based Discovery of Nonpeptide Allatostatin Analogues for Pest Control.

    PubMed

    Huang, Shan-Shan; Chen, Shan-Shan; Zhang, Hong-Ling; Yang, Han; Yang, Hui-Juan; Ren, Yu-Jie; Kai, Zhen-Peng

    2018-04-11

    FGLamide allatostatins (ASTs) are regarded as possible insecticide candidates, although their lack of in vivo effects, rapid degradation, poor water solubility, and high production costs preclude their practical use in pest control. In contrast to previous research, the C-terminal tripeptide (FGLa) was selected as the lead compound in this study. Five nonpeptide AST analogues (2-amino-1-[3-oxo-3-(substituted-anilino)propyl]pyridinium nitrate derivatives) were designed on the basis of the structure-activity relationship and docking results of FGLa. All of the nonpeptide analogues (S1-S5) were more potent against juvenile-hormone (JH) biosynthesis than the lead compound. They significantly inhibited the biosynthesis of JH in vivo following injection. A pest-control application demonstrated that S1 and S3 have larvicidal effects following oral administration (the IC 50 values were 0.020 and 0.0016 mg/g, respectively). The good oral toxicities and excellent water solubilities of S1 and S3 suggest that they have considerable potential as insecticides for pest management.

  8. Lepidopteran HMG-CoA reductase is a potential selective target for pest control.

    PubMed

    Li, Yuan-Mei; Kai, Zhen-Peng; Huang, Juan; Tobe, Stephen S

    2017-01-01

    As a consequence of the negative impacts on the environment of some insecticides, discovery of eco-friendly insecticides and target has received global attention in recent years. Sequence alignment and structural comparison of the rate-limiting enzyme HMG-CoA reductase (HMGR) revealed differences between lepidopteran pests and other organisms, which suggested insect HMGR could be a selective insecticide target candidate. Inhibition of JH biosynthesis in vitro confirmed that HMGR inhibitors showed a potent lethal effect on the lepidopteran pest Manduca sexta , whereas there was little effect on JH biosynthesis in Apis mellifera and Diploptera punctata . The pest control application of these inhibitors demonstrated that they can be insecticide candidates with potent ovicidal activity, larvicidal activity and insect growth regulatory effects. The present study has validated that Lepidopteran HMGR can be a potent selective insecticide target, and the HMGR inhibitors (especially type II statins) could be selective insecticide candidates and lead compounds. Furthermore, we demonstrated that sequence alignment, homology modeling and structural comparison may be useful for determining potential enzymes or receptors which can be eco-friendly pesticide  targets.

  9. Social reversal of immunosenescence in honey bee workers

    PubMed Central

    Amdam, Gro V.; Aase, Anne Lene T. O.; Seehuus, Siri-Christine; Fondrk, M. Kim; Norberg, Kari; Hartfelder, Klaus

    2008-01-01

    A striking example of immunosenescence is seen in the honey bee (Apis mellifera) worker caste. The bees’ age-associated transition from hive duties to more risky foraging activities is linked to a dramatic decline in immunity. Explicitly, it has been shown that an increase in the juvenile hormone (JH) level, which accompanies onset of foraging behavior, induces extensive hemocyte death through nuclear pycnosis. Here, we demonstrate that foragers that are forced to revert to hive-tasks show reversal of immunosenescence, i.e. a recovery of immunity with age. This recovery, which is triggered by a social manipulation, is accompanied by a drop in the endogenous JH titer and an increase in the hemolymph vitellogenin level. Vitellogenin is a zinc binding glycolipoprotein that has been implicated in the regulation of honey bee immune integrity. We also establish that worker immunosenescence is mediated by apoptosis, corroborating that reversal of immunosenescence emerges through proliferation of new cells. The results presented here, consequently, reveal a unique flexibility in honey bee immunity—a regulatory plasticity that may be of general biological interest. PMID:16169181

  10. Magnetic and metal-insulator transitions in coupled spin-fermion systems

    DOE PAGES

    Mondaini, R.; Paiva, T.; Scalettar, R. T.

    2014-10-14

    We use quantum Monte Carlo to determine the magnetic and transport properties of coupled square lattice spin and fermionic planes as a model for a metal-insulator interface. Specifically, layers of Ising spins with an intra-layer exchange constant J interact with the electronic spins of several adjoining metallic sheets via a coupling JH. When the chemical potential cuts across the band center, that is, at half-filling, the Neel temperature of antiferromagnetic (J > 0) Ising spins is enhanced by the coupling to the metal, while in the ferromagnetic case (J < 0) the metallic degrees of freedom reduce the ordering temperature.more » In the former case, a gap opens in the fermionic spectrum, driving insulating behavior, and the electron spins also order. This induced antiferromagnetism penetrates more weakly as the distance from the interface increases, and also exhibits a non-monotonic dependence on JH. For doped lattices an interesting charge disproportionation occurs where electrons move to the interface layer to maintain half-filling there.« less

  11. The JH1 Haplotype-a newly discovered marker for infertility in the jersy breed

    USDA-ARS?s Scientific Manuscript database

    The focus on production traits in genetic selection programs with little consideration for traits associated with reproduction has contributed to the decline in reproductive function. Moreover, there is a negative genetic correlation between milk yield and reproduction so that selection for yield ca...

  12. On Integrated Social and QoS Trust-Based Routing in Delay Tolerant Networks

    DTIC Science & Technology

    2011-05-15

    according to our protocol. This leads to Tik(t + At) < Tmin because /?j + f32 = 1 and T, fc (t) < Tmin is given (in the if part). Therefore, it is...Disruption-Tolerant Networking," IEEE INFOCOM 2006. Barcelona . Spain. April 2006, pp. 1-11. [3] J.H. Cho, A. Swami and I.R. Chen, "Modeling and

  13. Biotransformation of Toxic Metals by Bacteria

    DTIC Science & Technology

    1993-05-31

    toxic waste site" R.C. Blake, D.M. Choate, S.H. Bardhan , N.H. Revis, L.L. Barton, and T.G. Zocco; Environmental Toxicology and Chemistry, in press...34; R.C. Blake, D.M Choate, S.H. Bardhan , N.H. Revis, and J.H. Jackson; submitted to the Journal of Bioloaical Chemistry (iii) Tn preparation - none D

  14. Preliminary Study for the Modeling of an Artificial Icing Cloud.

    DTIC Science & Technology

    1983-08-01

    C.E. and Schulz, R.J., "Analytical Study of Icing Simulation for Turbine Engines in Altitude Test Cells". Arnold Engineering Devel- opment Center...Dept. SAMSO-TR-79-31, May 1979. 7. Keenan, J.H. and Keyes, F.G., "Thermodynamic Properties of Steam", John Wiley and Sons, Inc., N.Y., i961. 8. Pelton

  15. MATERNAL AND DEVELOPMENTAL TOXICITY OF PERFLUOROOCATANE SULFONATE (PFOS) IN THE RAT

    EPA Science Inventory

    MATERNAL AND DEVELOPMENTAL TOXICITY OF PERFLUOROOCTANE SULFONATE (PFOS) IN THE RAT. C. Lau1, J.M. Rogers1, J.R. Thibodeaux1, R.G. Hanson1, B.E. Grey1, B.D. Barbee1, J.H. Richards2, J.L. Butenoff3. 1Reprod. Tox. Div., 2Exp. Tox. Div., NHEERL, USEPA, Research Triangle Park, NC, 3...

  16. U.S. EPA, Pesticide Product Label, , 09/10/1982

    EPA Pesticide Factsheets

    2011-04-21

    ... h·fort'.1 I~, 1!:i Int:hi!~ Ii qh U,. fli11I,'/':H (i1o','I() lilt· t.". I l,t"·,1·>I(\\(; ,1/ 1',,', 1'JJ(Jh <;tat,e'i Rctlu. ,. S[)I.lY dl'" 11) kPI'p'''q ,t·,-I" I\\. 'II' . If ,11 "I,f ij, !,,. ...

  17. PHILLS-1 Hyperspectral Data Processing: 2001 LEO-15 Deployment

    DTIC Science & Technology

    2004-05-26

    2002. 4. R.A. Leathers, T.V. Downes, W.A. Snyder, J.H. Bowles, C.O. Curtiss, M.E. Kappus , W. Chen, D. Korwan, M.J. Montes, W.J. Rhea, and M.A. Carney...5. C. Davis, M. Kappus , J. Bowles, J. Fisher, J. Antoniades, and M. Carney, “Calibration, Characterization and First Results with the Ocean PHILLS

  18. Monoamine Oxidase A: A Novel Target for Progression and Metastasis of Prostate Cancer

    DTIC Science & Technology

    2013-10-01

    Paik, J.H. 2011. FoxO family members in cancer. Cancer biology & therapy 12:253-259. 31. Myatt, S.S., and Lam , E.W. 2007. The emerging roles of...J.B., Chen, K., Li, Y., Lau , Y.F., and Shih, J.C. 2009. Regulation of monoamine oxidase A by the SRY gene on the Y chromosome. FASEB journal

  19. A Proposal for Kelly CriterionBased Lossy Network Compression

    DTIC Science & Technology

    2016-03-01

    warehousing and data mining techniques for cyber security. New York (NY): Springer; 2007. p. 83–108. 34. Münz G, Li S, Carle G. Traffic anomaly...p. 188–196. 48. Kim NU, Park MW, Park SH, Jung SM, Eom JH, Chung TM. A study on ef- fective hash-based load balancing scheme for parallel nids. In

  20. Freedom to Make Choices for Health: Plus 40 Years

    ERIC Educational Resources Information Center

    Airhihenbuwa, Collins O.; Iwelunmor, Juliet

    2010-01-01

    In the 1969 inaugural issue of the "School Health Review", Douglass J.H. examined four major issues he felt were central to the question of choices one has about health: (1) problems with health care delivery methods; (2) persistent poverty in our population and its impact on health; (3) systemic problems inherent in social and institutional…

  1. Environmental Assessment: Construction Projects at the 189th Airlift Wing, Arkansas Air National Guard

    DTIC Science & Technology

    2003-09-12

    Magnet Cove and PrJtaJ~h Sulphur Springs, 1tocQ at G"~"anite Mountain a’lld ift. Saline Cou.11t],i, nd dike~ and sill.s (principalltl trachyt,f...and breccias (Benton), atui dWs and tiUs (principalllffourckite, 01£aehitiU, and moruJh-iqaiteJ OUACHITA MOUNTAINS REGION ~ SOAPSTONE -SERPENTINE

  2. Synthesis and antiprotozoan evaluation of new alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids.

    PubMed

    Coro, Julieta; Pérez, Rolando; Rodríguez, Hortensia; Suárez, Margarita; Vega, Celeste; Rolón, Miriam; Montero, David; Nogal, Juan José; Gómez-Barrio, Alicia

    2005-05-16

    Two new series of several alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids were synthesized in a two step procedure from the corresponding alkyl bis-dithiocarbamic salt intermediary. The novel compounds were evaluated for their activity in vitro against Trypanosoma cruzi strain CL (clone CL B5) and Trichomonas vaginalis strain JH 31A.

  3. Hormonal Interference with Pheromone Systems in Parasitic Acarines, Especially Ixodid Ticks.

    DTIC Science & Technology

    1983-05-01

    Genital Pheromone(s); Precocene-2; Hyalomma dromedarii; Dermacentor variabilis; Radioimmunoassay; juvenile hormone; Dermanyssus gallinae 19. KEY...dromedarii; Dermacentor variabilis; Radioimmunoassay; juvenile hormone; Dermanyssus gallinae . 20. A STRACT (Continue on reverse side I nfecessry and...allatotropin Precocene-2 and the juvenile hormone, JH3, on reporduction in the chicken mite, Dermanyssus gallinae is described. These studies are part of

  4. MATERNAL AND DEVELOPMENTAL TOXICITY OF PERFLUOROOCTANE SULFONATE (PFOS) IN THE MOUSE

    EPA Science Inventory

    MATERNAL AND DEVELOPMENTAL TOXICITY OF PERFLUOROOCTANE SULFONATE (PFOS) IN THE MOUSE. J.R. Thibodeaux1, R.G. Hanson1, B.E. Grey1, B.D. Barbee1, J.H. Richards2, J.L. Butenhoff3, J.M. Rogers1, C. Lau1. 1Reprod. Tox. Div., 2Exp. Tox. Div., NHEERL, ORD, US EPA, Research Triangle Pa...

  5. Infrared Color Selection of Massive Galaxies at z > 3

    NASA Astrophysics Data System (ADS)

    Wang, T.; Elbaz, D.; Schreiber, C.; Pannella, M.; Shu, X.; Willner, S. P.; Ashby, M. L. N.; Huang, J.-S.; Fontana, A.; Dekel, A.; Daddi, E.; Ferguson, H. C.; Dunlop, J.; Ciesla, L.; Koekemoer, A. M.; Giavalisco, M.; Boutsia, K.; Finkelstein, S.; Juneau, S.; Barro, G.; Koo, D. C.; Michałowski, M. J.; Orellana, G.; Lu, Y.; Castellano, M.; Bourne, N.; Buitrago, F.; Santini, P.; Faber, S. M.; Hathi, N.; Lucas, R. A.; Pérez-González, P. G.

    2016-01-01

    We introduce a new color selection technique to identify high-redshift, massive galaxies that are systematically missed by Lyman-break selection. The new selection is based on the H160 (H) and Infrared Array Camera (IRAC) 4.5 μm bands, specifically H-[4.5]\\gt 2.25 mag. These galaxies, called “HIEROs,” include two major populations that can be separated with an additional J - H color. The populations are massive and dusty star-forming galaxies at z\\gt 3 ({JH}-{blue}) and extremely dusty galaxies at z≲ 3 ({JH}-{red}). The 350 arcmin2 of the GOODS-North and GOODS-South fields with the deepest Hubble Space Telescope (HST)/Wide Field Camera 3 (WFC3) near-infrared and IRAC data contain as many as 285 HIEROs down to [4.5]\\lt 24 mag. Inclusion of the most extreme HIEROs, not even detected in the H band, makes this selection particularly complete for the identification of massive high-redshift galaxies. We focus here primarily on {JH}-{blue} (z\\gt 3) HIEROs, which have a median photometric redshift < z> ˜ 4.4 and stellar mass {M}*˜ {10}10.6 {M}⊙ and are much fainter in the rest-frame UV than similarly massive Lyman-break galaxies (LBGs). Their star formation rates (SFRs), derived from their stacked infrared spectral energy distributions (SEDs), reach ˜240 {M}⊙ yr-1, leading to a specific SFR, {{sSFR}}\\equiv {{SFR}}/{M}*˜ 4.2 Gyr-1, suggesting that the sSFRs for massive galaxies continue to grow at z\\gt 2 but at a lower growth rate than from z = 0 to z = 2. With a median half-light radius of 2 kpc, including ˜ 20% as compact as quiescent (QS) galaxies at similar redshifts, {JH}-{blue} HIEROs represent perfect star-forming progenitors of the most massive ({M}*≳ {10}11.2 {M}⊙ ) compact QS galaxies at z˜ 3 and have the right number density. HIEROs make up ˜ 60% of all galaxies with {M}*\\gt {10}10.5 {M}⊙ identified at z\\gt 3 from their photometric redshifts. This is five times more than LBGs with nearly no overlap between the two populations

  6. Identifying microRNAs that Regulate Neuroblastoma Cell Differentiation

    DTIC Science & Technology

    2014-09-01

    Watters, K. M., Das, S., Bryan, K., Bernas, T., Prehn , J. H., and Stallings, R. L. (2011) MicroRNAs 10a and 10b are potent inducers of neuroblastoma...Cell Biol 2009;29(19):5290-5305. 30. Foley NH, Bray I, Watters KM, Das S, Bryan K, Bernas T, Prehn JH, Stallings RL. MicroRNAs 10a and 10b are potent

  7. Preparation of Copper (Cu)-Nickel (Ni) Alloy Thin Films for Bilayer Graphene Growth

    DTIC Science & Technology

    2016-02-01

    public release; distribution is unlimited. 5 0 50 100 150 200 250 300 350 40 60 80 100 In te ns ity 2 Theta (°) 6:1 Cu/Ni, 15 mT, 400°C...JY, Hong BH. Large-scale pattern growth of graphene films for stretchable transparent electrodes. Nature. 2009;457:706−710 4. Li XS , Cai WW, An JH

  8. Analysis and Synthesis of Adaptive Neural Elements and Assembles

    DTIC Science & Technology

    1992-02-17

    effects of neuromodulators on electrically activity. Based on the simulations it appears that there are potentially novel mechanisms with which modulatory...and Byrne, J.H. A learning rule based on empirically-derived activity-dependent neuromodulation supports operant conditioning in a small network...dependent neuromodulation can support operant conditioning in a small oscillatory network". 2. Society for Neuroscience Short Course on Neural

  9. Development of Silicon-Coated Superparamagnetic Iron Oxide Nanoparticles for Targeted Molecular Imaging and Hyperthermic Therapy of Prostate Cancer

    DTIC Science & Technology

    2015-08-01

    nanoparticles have received recent interest as targeted diagnostic and drug delivery vehicles, due to their biocompatibility, biodegradability , and...vivo tumor diagnosis. Cancer Research 66, 10855–10860 (2006). 20. Park, J.-H. et al. Biodegradable luminescent porous silicon nanoparticles for in...biocompatibility, biodegradability , and simple surface chemistry that is amenable to drug loading and targeting. A method of hyperpolarizing silicon particles

  10. Research Area 4.1 Nano- and Bio-Electronics: Lester Eastman Conference on High-Performance Devices

    DTIC Science & Technology

    2017-06-02

    temperatures higher than 1600 oC, in-situ generation of carbon vacancies overcomes the effect of carbon implantation, and results in a The authors would like...passivation layers are used in these measurements. B. High Temperature Oxidation The effect of high temperature oxidation treatment on carrier... Temperature Characteristics of In0.7 Ga0.3As PHEMTs ............................................................................................12 S‐W Son, J.H

  11. Demonstration of a Fractured Rock Geophysical Toolbox (FRGT) for Characterization and Monitoring of DNAPL Biodegradation in Fractured Rock Aquifers

    DTIC Science & Technology

    2016-01-01

    USER’S GUIDE Demonstration of a Fractured Rock Geophysical Toolbox (FRGT) for Characterization and Monitoring of DNAPL Biodegradation in...Toolbox (FRGT) for Characterization and Monitoring of DNAPL Biodegradation in Fractured Rock Aquifers F.D. Day-Lewis, C.D. Johnson, J.H. Williams, C.L...are doomed to failure. DNAPL biodegradation charactrization and monitoring, remediation, fractured rock aquifers. Unclassified Unclassified UU UL 6

  12. Hormonal interference with pheromone systems in parasitic acarines, especially ixodid ticks. Annual technical report No. 4, 1 May 1983-30 April 1984

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sonenshine, D.E.; Oliver, J.H. Jr.; Homsher, P.J.

    1984-05-01

    The most important result of recent project research was the demonstration of the juvenoid JH III by radioimmunoassay. This assay revealed an estimated 78 pg/tick in the hemolymph of partially fed Hyalomma dromedarii females, and an estimated 3 pg/tick in the hemolymph of partially fed D. variabilis. Other studies, especially digestion of tritium labelled JH III, provided additional evidence suggesting the presence of this hormone in adult ticks. The implications of these findings for our understanding of sex pheromone regulation in ticks is discussed. Other studies described in this report deal with the source of ecdysteroid in teh camel tick,more » Hyalomma dromedarii, the American dog tick, Dermacentor variabilis, and the soft tick, Ornithodoros parkeri. Studies done at ODU, using radioimmunoassay high performance liquid chromatography, and autoradiography, provide new evidence implicating the tick synganglion - lateral nerve plexus as an important site of ecdysteroid activity in the ixodid ticks. Other studies with ecdysteriods suggest that metabolism of ecdysone or 20-hydroxyecdysone (or both) to inactive metabolites, possibly including polar conjugates. If confirmed, these findings indicate the presence of only a single active ecdysteriod hormone in ticks, 20-hydroxyecdysone.« less

  13. Effects of treatment of the fat body trophocytes of Melipona quadrifasciata anthidioides nurse workers and virgin queens in culture by juvenile hormone III and ecdysterone (20-HE).

    PubMed

    Paes-De-Oliveira, Vagner Tadeu; Berger, Bruno; Poiani, Silvana Beani; Paulino Simões, Zilá Luz; Da Cruz-Landim, Carminda

    2013-01-01

    The fat body (FB) consists of two types of cells: throphocytes and oenocytes. Throphocytes are related to intermediary metabolism storing lipids, carbohydrates, and proteins while oenocytes play role in the lipids and lipoproteins production. The vitellogenin is the precursor of egg yolk (vitelline) and is synthesized on FB. The aim of this work was to analyze the effects of hormones acting in bee reproduction, as juvenile hormone (JH) and ecdisteroids (20 HE) on FB cells, where vitellogenin is synthesized. For the study were chose nurse workers that in Melipona quadrifasciata anthidioides present activated ovaries and produce eggs, and virgin queens whose ovaries are not yet activated, presenting only previtellogenic follicles. FB trophocytes from these classes of bees were cultivated in media containing different amounts of JH and 20-HE. The effects on trophocytes cytoplasm reserves of lipids, proteins, and activity of acid phosphatase were compared by observing preparations from cultured FB, treated and control, by transmission electron microscopy (TEM). The results showed that the hormones effects are related to the bee's caste and functional ovary stage. The role of acid phosphatase on mobilization of the trophocyte reserves was also determined. Copyright © 2012 Wiley Periodicals, Inc.

  14. The TUNEL assay suggests mandibular regression by programmed cell death during presoldier differentiation in the nasute termite Nasutitermes takasagoensis

    NASA Astrophysics Data System (ADS)

    Toga, Kouhei; Yoda, Shinichi; Maekawa, Kiyoto

    2011-09-01

    Termite soldiers are the most specialized caste of social insects in terms of their morphology and function. Soldier development requires increased juvenile hormone (JH) titer and the two molts via a presoldier stage. These molts are accompanied by dramatic morphological changes, including the exaggeration and regression of certain organs. Soldiers of the most apical termitid subfamily Nasutitermitinae possess not only a horn-like frontal tube, called the nasus, for the projection of defensive chemicals from the frontal gland reservoir but also regressed mandibles. Although candidate genes regulating soldier mandibular growth were reported in a relatively basal termite species, the regulatory mechanisms of mandibular regression remain unknown. To clarify these mechanisms, we performed morphological and histological examinations of the mandibles during soldier differentiation in Nasutitermes takasagoensis. Mandibular size reduced dramatically during soldier differentiation, and mandibular regression occurred just prior to the presoldier molt. Spotted TUNEL signals were observed in regressing mandibles of presoldiers, suggesting that the regression involved programmed cell death. Because soldiers of N. takasagoensis possess exaggerated organs (nasus and frontal gland), the present results suggest that JH-dependent regressive mechanisms exist in the mandibles without interfering with the formation of the exaggerated organs.

  15. Larval leg integrity is maintained by Distal-less and is required for proper timing of metamorphosis in the flour beetle, Tribolium castaneum

    PubMed Central

    Suzuki, Yuichiro; Squires, Diego C.; Riddiford, Lynn M.

    2009-01-01

    The dramatic transformation from a larva to an adult must be accompanied by a coordinated activity of genes and hormones that enable an orchestrated transformation from larval to pupal/adult tissues. The maintenance of larval appendages and their subsequent transformation to appendages in holometabolous insects remains elusive at the developmental genetic level. Here the role of a key appendage patterning gene Distal-less (Dll) was examined in mid- to late- larval stages of the flour beetle, Tribolium castaneum. During late larval development, Dll was expressed in appendages in a similar manner as previously reported for the tobacco hornworm, Manduca sexta. Removal of this late Dll expression resulted in disruption of adult appendage patterning. Intriguingly, earlier removal resulted in dramatic loss of structural integrity and identity of larval appendages. A large amount of variability in appendage morphology was observed following Dll dsRNA injection, unlike larvae injected with dachshund dsRNA. These Dll dsRNA-injected larvae underwent numerous supernumerary molts, which could be terminated with injection of either JH methyltransferase or Methoprene-tolerant dsRNA. Apparently, the partial dedifferentiation of the appendages in these larvae acts to maintain high JH and, hence, prevents metamorphosis. PMID:19022238

  16. Children’s Hospital of Pittsburgh and Diabetes Institute of the Walter Reed Health Care System Genetic Screening in Diabetes: Candidate Gene Analysis for Diabetic Retinopathy

    DTIC Science & Technology

    2008-05-22

    available and consents to be in the study. • In addition to referrals from Diabetes Institute nurse practitioners, endocrinologists, and diabetes ...coronary artery disease in juvenile - onset, insulin dependent diabetes mellitus. Am J Cardiol. 59:750-755, 1987a. Krolewski AS, Warram JH, Rand LI...1-0313 TITLE: Children’s Hospital of Pittsburgh and Diabetes Institute of the Walter Reed Health Care System Genetic Screening in

  17. Medical and Non-Medical Predictors of Disability Discharge Disposition for Navy Personnel with a Back Problem: A Focus on Entitlement

    DTIC Science & Technology

    1988-03-28

    Washington D.C. 20350-1000. Stryker, S., & Gottlieb, A. (1981). Attribution theory and symbolic interactionism : A comparison. In J.H. Howes, W...ERFORMIING ORGANIZATION 6b OFFiCE SYMBOL 7a NAME OF MONITORING ORGANIZATION (If applicable) Naval Health Research Center 40 Commander, Naval Medical Command...Washington, DC 20372 ea NAME OF FUNDING/SPONSORING Bb OFFICE SYMBOL 9. PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER ORGANIZATION Naval Medical (If applicable

  18. Carrion use by northern goshawks

    Treesearch

    John R. Squires

    1995-01-01

    Northern goshawks (Accipiter gentilis) feed on a wide variety of birds and mammals (R. T. Reynolds, et al. 1992, USDA For. Ser. Gen. Tech. Rep. RM-217. Fort Collins, CO U.S.A.), but few accounts describe goshawks feeding on carrion. J.H. Schnell (1958, Condor 60:377-403) stated that, "It seems highly unlikely that the goshawk would forage for carrion under normal...

  19. The Use of Satellite Observed Cloud Patterns in Northern Hemisphere 300 mb and 1000/300 mb Numerical Analysis.

    DTIC Science & Technology

    1984-02-01

    prediction Extratropical cyclones Objective analysis Bogus techniques 20. ABSTRACT (Continue on reverse aide If necooearn mid Identify by block number) Jh A...quasi-objective statistical method for deriving 300 mb geopotential heights and 1000/300 mb thicknesses in the vicinity of extratropical cyclones 0I...with the aid of satellite imagery is presented. The technique utilizes satellite observed extratropical spiral cloud pattern parameters in conjunction

  20. Development of Antibacterials Targeting the MEP Pathway of Select Agents

    DTIC Science & Technology

    2013-02-01

    based assays for lead inhibitor discovery, evaluation of lead inhibitors in microbial growth assays, determining X- ray crystal structures of MEP pathway...inhibitors. • On-demand production and delivery of recombinant proteins to WRAIR for X- ray crystallography. Reportable Outcomes...characterization and phosphoregulation. PLoS ONE 6: e20884. doi:10.1371/journal.pone.0020884. 3. Zhang JH, Chung TD, Oldenburg KR (1999) A Simple

  1. 14. DREDGING MAP. United Engineering Company Ltd., Alameda Shipyard. Ship ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. DREDGING MAP. United Engineering Company Ltd., Alameda Shipyard. Ship repair facilities dredging map. No architect noted. Drawn by "J.H." (John Hudspeth?). Sheet 1. Plan no. 10,529. Scale one inch to 50 feet. September 22, 1943. U.S. Navy, Bureau of Yards & Docks, Contract no. bs 76. Approved for construction October 18, 1943. blueprint - United Engineering Company Shipyard, 2900 Main Street, Alameda, Alameda County, CA

  2. Implicit Learning of Complex Visual Contexts Under Non-Optimal Conditions

    DTIC Science & Technology

    2007-07-27

    Perception & Performance, 31(6), 1439-1448. 3. Jiang Y, Song J-H, Rigas A (2005). High-capacity spatial contextual memory. Psychonomic Bulletin & Review , 12...Makovski T., & Jiang YV (in press). Distributing versus focusing attention in visual short-term memory. Psychonomic Bulletin & Review . 8. Rausei V...Implicit learning of ignored visual context. Psychonomic Bulletin & Review , 12(1), 100-106. Jiang, Y. H., & Song, J. H. (2005). Spatial context

  3. The Use of Position-Tracking Drifters in Riverine Environments

    DTIC Science & Technology

    2010-06-01

    provide information of the particle pathways and material transport for sediment, biotic , abiotic and pollutants . Moreover, drifter position data can...Measurements. Proceedings, Lamont Geological Observatory Symposium on Diffusion in Oceans and Fresh Waters. Pallisades, N.Y., 1964-1965. pp. 1-18. [3...38(8), 927-957. [19] LaCasce, J.H., 2008. Lagrangian statistics from oceanic and atmospheric observations. Lect. Notes Phys. 744, 165-218. [20

  4. Investigation of europium(III)-doped ZnS for immunoassay

    NASA Astrophysics Data System (ADS)

    Zhu, Chao-Fan; Sha, Xue; Chu, Xue-Ying; Li, Jin-Hua; Xu, Ming-Ze; Jin, Fang-Jun; Xu, Zhi-Kun

    2018-02-01

    Not Available Project supported by the National Natural Science Foundation of China (Grant No. 61205193), the Project of Science and Technology of Jilin Province, China (Grant No. 20140520107JH), the Technology Foundation of Jilin Provincial Department of Human Resources and Social Security, China (Grant No. RL201306), and the Science Foundation for Young Scientists of Changchun University of Science and Technology, China (Grant No. XQNJJ-2015-03).

  5. Anatomy of a Natural Composite Material

    DTIC Science & Technology

    1989-11-01

    from the vitellaria of the liver fluke, Fasciola hepatica. Biochemistry 28, 6104-6110. 2. Waite, J.H., Hansen, D.C., & Little, K.T. (1989) The glue...certainly belong to distinct genera and inhabit distinct ecological niches. Adhesive protein sequences from two non-molluscan species, Fasciola hepatica...choros), Gd (Geukensia demissa), Fh ( Fasciola hepatica), Me (Mytilus edulis), Mg (Mytella guyanensis), Mms (Modiolus modiolus squamosus), Pc

  6. Archaeological Testing of the Confederate Obstructions. 1Mb28, Mobile Harbor, Alabama.

    DTIC Science & Technology

    1985-07-01

    Historical Society of America, Staten Island, New York. 58 - . . -. . Maury, Dabney H. - 1864 Statement Concerning Placement of Torpedoes Across...Minnesota Historical Society , St. Paul. Merrill, Col. W. E. 1866 Report on the Present Condition of the Harbor of Mobile. MSon file, National...194. Polk, James K. 1971 Pensacola Commerce and Industry: 1821-1860. MSon file, Pensacola Historical Society , Pensacola. Scruggs, J.H., Jr. 1953

  7. Proceedings of the Annual Conference on Manual Control (10th) held at Wright-Patterson AFB, Ohio on 9-11 April 1974

    DTIC Science & Technology

    1974-04-01

    A Jh .*H-M,fcflTlt FUTURE TERMINAL AIR TRAFFIC MANAGEMENT CONCEPTS John G. Kreifeldt Department of Engineering Design Tufts University,Medford...wright-patterson afb,ohio Hair force institute of technology > air force flight dynamics laboratory 1 V& t \\ jfr£ Approved for public release...report should not be returned unless return is required by security considerationsi contractual obligations, or notice on a specific document. AIR

  8. Characterization and Expression of Drug Resistance Genes in MDROs Originating from Combat Wound Infections

    DTIC Science & Technology

    2014-10-01

    media so only adherent/close proximity bacterial cells were harvested . Cells were then treated with RNAprotect Cell Reagent (Qiagen) to immediately...2004, 32:2386-2395. 4. Chen Y, Stine OC, Badger JH, Gil AI, Nair GB, Nishibuchi M, Fouts DE: Comparative Genomic Analysis of Vibrio ...antigen biosynthesis regions in Vibrio cholerae. Applied and environmental microbiology 2011, 77:2247-2253. 6. Jacobsen A, Hendriksen RS, Aaresturp FM

  9. Detail of plaque beneath column on the south parapet at ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of plaque beneath column on the south parapet at the west end of the bridge. The plaque reads “1914; Mayor E.J. Drussel; Councilmen E.S. Henry, E.F. Hogan, R.P. Lamdin, C.F. Ross, J.H. Shuppert; Leonard & Day, Engineers; C.H. Gildersleeve, Builder.” - First Street Bridge, Spanning Napa River at First Street between Soscol Avenue & Juarez Street, Napa, Napa County, CA

  10. Molecular determinants of caste differentiation in the highly eusocial honeybee Apis mellifera.

    PubMed

    Barchuk, Angel R; Cristino, Alexandre S; Kucharski, Robert; Costa, Luciano F; Simões, Zilá L P; Maleszka, Ryszard

    2007-06-18

    In honeybees, differential feeding of female larvae promotes the occurrence of two different phenotypes, a queen and a worker, from identical genotypes, through incremental alterations, which affect general growth, and character state alterations that result in the presence or absence of specific structures. Although previous studies revealed a link between incremental alterations and differential expression of physiometabolic genes, the molecular changes accompanying character state alterations remain unknown. By using cDNA microarray analyses of >6,000 Apis mellifera ESTs, we found 240 differentially expressed genes (DEGs) between developing queens and workers. Many genes recorded as up-regulated in prospective workers appear to be unique to A. mellifera, suggesting that the workers' developmental pathway involves the participation of novel genes. Workers up-regulate more developmental genes than queens, whereas queens up-regulate a greater proportion of physiometabolic genes, including genes coding for metabolic enzymes and genes whose products are known to regulate the rate of mass-transforming processes and the general growth of the organism (e.g., tor). Many DEGs are likely to be involved in processes favoring the development of caste-biased structures, like brain, legs and ovaries, as well as genes that code for cytoskeleton constituents. Treatment of developing worker larvae with juvenile hormone (JH) revealed 52 JH responsive genes, specifically during the critical period of caste development. Using Gibbs sampling and Expectation Maximization algorithms, we discovered eight overrepresented cis-elements from four gene groups. Graph theory and complex networks concepts were adopted to attain powerful graphical representations of the interrelation between cis-elements and genes and objectively quantify the degree of relationship between these entities. We suggest that clusters of functionally related DEGs are co-regulated during caste development in honeybees

  11. Clonal immunoglobulin heavy chain and T-cell receptor γ gene rearrangements in primary gastric lymphoma.

    PubMed

    Shan, Guo-Dong; Hu, Feng-Ling; Yang, Ming; Chen, Hong-Tan; Chen, Wen-Guo; Wang, Yun-Gui; Chen, Li-Hua; Li, You-Ming; Xu, Guo-Qiang

    2013-09-14

    To study the diagnostic value of immunoglobulin heavy chain (IgH) and T-cell receptor γ (TCR-γ) gene monoclonal rearrangements in primary gastric lymphoma (PGL). A total of 48 patients with suspected PGL at our hospital were prospectively enrolled in this study from January 2009 to December 2011. The patients were divided into three groups (a PGL group, a gastric linitis plastica group, and a benign gastric ulcer group) based on the pathological results (gastric mucosal specimens obtained by endoscopy or surgery) and follow-up. Endoscopic ultrasonography (EUS) and EUS-guided biopsy were performed in all the patients. The tissue specimens were used for histopathological examination and for IgH and TCR-γ gene rearrangement polymerase chain reaction analyses. EUS and EUS-guided biopsy were successfully performed in all 48 patients. In the PGL group (n = 21), monoclonal IgH gene rearrangements were detected in 14 (66.7%) patients. A positive result for each set of primers was found in 12 (57.1%), 8 (38.1%), and 4 (19.0%) cases using FR1/JH, FR2/JH, and FR3/JH primers, respectively. Overall, 12 (75%) patients with mucosal-associated lymphoid tissue lymphoma (n = 16) and 2 (40%) patients with diffuse large B-cell lymphoma (n = 5) were positive for monoclonal IgH gene rearrangements. No patients in the gastric linitis plastica group (n = 17) and only one (10%) patient in the benign gastric ulcer group (n = 10) were positive for a monoclonal IgH gene rearrangement. No TCR-γ gene monoclonal rearrangements were detected. The sensitivity of monoclonal IgH gene rearrangements was 66.7% for a PGL diagnosis, and the specificity was 96.4%. In the PGL group, 8 (100%) patients with stage IIE PGL (n = 8) and 6 (46.1%) patients with stage IE PGL (n = 13) were positive for monoclonal IgH gene rearrangements. IgH gene rearrangements may be associated with PGL staging and may be useful for the diagnosis of PGL and for differentiating between PGL and gastric linitis plastica.

  12. The acute effects of manipulating volume and load of back squats on countermovement vertical jump performance.

    PubMed

    Moir, Gavin L; Mergy, David; Witmer, Ca; Davis, Shala E

    2011-06-01

    The acute effects of manipulating the volume and load of back squats on subsequent countermovement vertical jump performance were investigated in the present study. Eleven National Collegiate Athletic Association division II female volleyball players performed 10 countermovement vertical jumps (CMJs) on a force platform 2 minutes after the last squat repetition of a high-load (HL) or high-volume (HV) squat protocol. Two minutes of rest was provided between each CMJ. The HL protocol culminated in the subjects having to perform 3 repetitions with a load equivalent to 90% 1 repetition maximum (1RM) back squat, whereas 12 repetitions with a load equivalent to 37% 1RM were performed in the HV protocol. During an initial familiarization session, knee angles were recorded during a series of CMJs, and these angles were used to control the depth of descent during all subsequent back squats. Jump height (JH) and vertical stiffness (VStiff) were calculated during each of the 10 CMJ, and the change in these variables after the 2 squat protocols was assessed using an analysis of variance model with repeated measures on 2 factors (Protocol [2-levels]; Time [2-levels]). There was no significant difference in JH after the HL and HV protocols (p > 0.05). A significant Protocol × Time interaction for VStiff resulted from the increase after the HL protocol being greater than that after the HV protocol (p = 0.03). The knee angles before the HL and HV protocols were significantly greater than those measured during the initial familiarization session (p = 0.001). Although neither squat protocol provided any benefit in improving JH, the heavy squat protocol produced greater increases in VStiff during the CMJ. Because of the increased VStiff caused by the HL protocol, volleyball coaches may consider using such protocols with their players to improve performance in jumps performed from a run such as the spike and on-court agility.

  13. Molecular determinants of caste differentiation in the highly eusocial honeybee Apis mellifera

    PubMed Central

    Barchuk, Angel R; Cristino, Alexandre S; Kucharski, Robert; Costa, Luciano F; Simões, Zilá LP; Maleszka, Ryszard

    2007-01-01

    Background In honeybees, differential feeding of female larvae promotes the occurrence of two different phenotypes, a queen and a worker, from identical genotypes, through incremental alterations, which affect general growth, and character state alterations that result in the presence or absence of specific structures. Although previous studies revealed a link between incremental alterations and differential expression of physiometabolic genes, the molecular changes accompanying character state alterations remain unknown. Results By using cDNA microarray analyses of >6,000 Apis mellifera ESTs, we found 240 differentially expressed genes (DEGs) between developing queens and workers. Many genes recorded as up-regulated in prospective workers appear to be unique to A. mellifera, suggesting that the workers' developmental pathway involves the participation of novel genes. Workers up-regulate more developmental genes than queens, whereas queens up-regulate a greater proportion of physiometabolic genes, including genes coding for metabolic enzymes and genes whose products are known to regulate the rate of mass-transforming processes and the general growth of the organism (e.g., tor). Many DEGs are likely to be involved in processes favoring the development of caste-biased structures, like brain, legs and ovaries, as well as genes that code for cytoskeleton constituents. Treatment of developing worker larvae with juvenile hormone (JH) revealed 52 JH responsive genes, specifically during the critical period of caste development. Using Gibbs sampling and Expectation Maximization algorithms, we discovered eight overrepresented cis-elements from four gene groups. Graph theory and complex networks concepts were adopted to attain powerful graphical representations of the interrelation between cis-elements and genes and objectively quantify the degree of relationship between these entities. Conclusion We suggest that clusters of functionally related DEGs are co-regulated during

  14. Far Forward Battlefield Telemedicine: Ultrasonic Guidance in Diagnosis and Emergency Therapeutics

    DTIC Science & Technology

    2006-08-01

    bicuspid aortic stenosis . J Am Soc Echocardiogr. 2005 Dec;18(12):1392-8. 44. Eto Y, Yamada H, Shin JH, Agler DA, Tsujino H, Qin JX, Saracino G, Greenberg...nitroprusside in aortic stenosis associated with severe heart failure: pressure- volume loop analysis using a numerical model. Am J Physiol Heart Circ...3D echocardiography, including exercise and intraoperative (epicardial) examinations, with quantitative validation in aneurysmal ventricles,3 aortic

  15. Advanced High Cycle Fatigue (HCF) Life Assurance Methodologies

    DTIC Science & Technology

    2004-07-01

    Fatigue in 2024 - T351 Aluminum Alloy , Wear, 221(1), pp 24-36 (1998) 27. Doner, M., Bain, K.R., and Adams, J.H... alloy , PWA 1484, where temperature and orientation effects both have to be taken into account. Both fracture mechanics and fatigue life methods... effect on predicted fatigue life . On average, the fatigue life is several orders of magnitude less when residual stresses are included. The

  16. Recommendations for Model Driven Paradigms for Integrated Approaches to Cyber Defense

    DTIC Science & Technology

    2017-03-06

    analogy (e.g., Susceptible, Infected, Recovered [SIR]) • Abstract wargaming: game -theoretic model of cyber conflict without modeling the underlying...malware. 3.7 Abstract Wargaming Here, a game -theoretic process is modeled with moves and effects inspired by cyber conflict but without modeling the...underlying processes of cyber attack and defense. Examples in literature include the following: • Cho J-H, Gao J. Cyber war game in temporal networks

  17. Evaluation of a PK/PBAN Analog with an (E)-Alkene, trans-Pro Isostere Identifies the Pro Orientation for Activity in Four Diverse PK/PBAN Bioassays

    DTIC Science & Technology

    2009-01-01

    member of the family, leucopyrokinin (LPK), was isolated from the cockroach Leucophaea maderae [12] with over 30members of this peptide class...myotropic bioassays The results of an evaluation of trans-Pro isosteric analog PK- Etz in the larval pupariation assay of N. bullata and isolated ...001-01R) (RJN). References [1] Abernathy RL, Nachman RJ, Teal PEA , Yamashita O, Tumlinson JH. Pheromo- notropic activity of naturally-occurring

  18. Molecular Strategies Against Sulfur Mustard Toxicity

    DTIC Science & Technology

    2010-04-01

    Stephenson JM, Abrahamian H, Keipes M, Castellarin A, Rogulja- Pepeonik Z, Fuller JH. Effect of lisinopril on progression of retinopathy in normotensive...people with type 1 diabetes . The EUCLID Study Group. EURODIAB Controlled Trial of Lisinopril in Insulin- Dependent Diabetes Mellitus. Lancet 1998;351:28...metabolic syndrome: new perspective against the epidemic. Diabetes 2005;54:1899. [46] McLachlan JA, Burow M, Chiang TC, Li SF. Gene imprinting in

  19. Placebo Controlled Study of Repetitive Transcranial Magnetic Stimulation for the Treatment of Parkinson’s Disease

    DTIC Science & Technology

    2004-03-01

    References Aarsland D., Larsen J.P., Waage 0., Langeveld J.H. Maintenance electroconvulsive therapy for Parkinson’s disease. Convuls Ther. 1997; 13: 274-277...following electroconvulsive therapy . 1: Psychopharmacology (Berl). 1982; 76: 371-376. Belmaker R.H., Grisaru N. Magnetic stimulation of the brain in...rTMS might have any long-lasting therapeutic effects (weeks or months) in persons with PD who are already receiving optimal available therapy

  20. The Feasibility of Oil Analysis for Air Force Diesel Engines

    DTIC Science & Technology

    1979-06-01

    FERROGRAPHY ...... ........ .............. oe..*8 BILORPY......... 3 -. LIST OF ILLUSTRATIONS Figure Page 1 End Item Inventory by EquipmentType...Wearmetal (General) a Assessment, Fluid 0 Condition, Fluid • Corrosion 0 • Debris, Wear 63 i’ ’J ’ Ii 4 0 Diagnosis, Machine * Ferrography * Filters...Paper No. 770829. 36. Hofman, M.V., and Johnson, J.H. (Michigan Technological Univ.), "The Development and Application of Ferrography to the Study of

  1. Mathematical Modeling of Physical and Cognitive Performance Decrement from Mechanical and Inhalation Insults

    DTIC Science & Technology

    2009-12-01

    INHALATION TOXICOLOGY RESEARCH 2.1.1 Development of a Fatigue Model & Blood Oxygen-based Parameter Corre- lates Liu et al. (2002) introduced a muscle ...and Stuhmiller, J.H. “Generalization of a ‘phenomenological’ muscle fatigue model.” Technical report J0287-10-382 (in preparation). Product 3. Sih...physiologic response to exercise and a model of muscle fatigue which have been developed and validated separately are integrated. Integration occurs through

  2. The Treatment of BRCA1/2 Hereditary Breast Cancer and Sporadic Breast Cancer with Poly(ADP-ribose) PARP-1 Inhibitors and Chemotherapy

    DTIC Science & Technology

    2008-09-01

    American population d) D) Obesity, and breast cancer J. of Nursing and Bariatric Surgery . 2008 submitting. This paper uses in part mechanisms worked...National Med. Society. 2008 submitting D) Obesity, and breast cancer J. of Nursing and Bariatric Surgery . 2008 submitting Abstracts: A) De Soto JA...submitting De Soto JA. Obesity, breast cancer and bariatric surgery . J. of Nursing and Bariatric Surgery . 2008 submitting Davis JH, De Soto JA

  3. Basic Studies of Distributed Discharge Limiters

    DTIC Science & Technology

    2014-02-10

    Sputtered Lanthanum Hexaboride Film Thickness on Field Emission from Metallic Knife Edge Cathodes,” M.P. Kirley, B. Novakovic , N. Sule, M. J. Weber, I...IEEE ICOPS, San Diego, CA (2009). 99. Nishant Sule, Matt Kirley, Bozidar Novakovic , John Scharer, Irena Knezevic and John H. Booske...M. Kirley, B. Novakovic , J. Scharer, I. Knezevic, and J.H. Booske, “Field emission from low work function cathode coatings,”, Intl. Conf. Plasma

  4. Rational Design of Rho Protein Inhibitors

    DTIC Science & Technology

    2006-09-01

    X- ray crystallographic techniques for structure determination. This training regimen has consisted of formal training either individually from...facility (Brenda Temple, Ph.D.), members of the LDDN at Harvard University (Ross Stein, Ph.D. and Li-An Yeh, Ph.D.), the director of the X- ray core...initiation but essential for metastasis. Genes Dev, 2005. Electronic publication ahead of print. 23. Zhang J-H, Chung TDY, and Oldenburg KR. A

  5. Floating Ocean Platform

    DTIC Science & Technology

    2003-08-15

    floating structures create novel habitats for subtidal epibiota?, MARINE ECOLOGY -PROGRESS SERIES, 43-52 Mar. Ecol.- Prog. Ser., 2002 Vegueria, SFJ Godoy... ECOLOGICAL APPLICATIONS, 350-366 Ecol. Appl., 2000 Niedzwecki, JM van de Lindt, JW Gage, JH Teigen, PS, Design estimates of surface wave interaction with...The ecological effects beyond the offshore platform, Coastal Zone: Proceedings of the Symposium on Coastal and Ocean Management, v 2, n pt2, 1989, p

  6. CG-MS/MS Analyses of Biological Samples in Support of Developmental Toxic Effects on Whole-Body Exposure of Rats to GB

    DTIC Science & Technology

    2015-03-01

    Sensitivity to Organophosphorous Anticholinesterase Compounds. Prog. Neurobiol. 1987, 28, 97–129. Shih, T-M.; Penetar, D.M.; McDonough, J.H. Jr.; Romano...J.A.; King, J.M. Age- related Differences in Soman Toxicity and in Blood and Brain Regional Cholinesterase Activity . Brain Res. Bull. 1990, 24...Organophosphates. Toxicol. Appl. Pharmacol. 2004, 198, 132–151. Sterri, S.H.; Berge, G.; Fonnum, F. Esterase Activities and Soman Toxicity in

  7. ISPI | CTIO

    Science.gov Websites

    Visitor's Computer Guidelines Network Connection Request Instruments Instruments by Telescope IR Instruments ‹› You are here CTIO Home » Astronomers » Instruments » IR Instruments » ISPI ISPI The CTIO matched to f/8 IR image quality of ~0.6 arcsec 10.25 x 10.25 arcmin field of view, broad band J,H and Ks

  8. Fundamental Automated Scheduling System (FASS): A Second Look.

    DTIC Science & Technology

    1986-12-01

    Mr. J.H. Shoemaker and Mr. Ron Flatley of Norfolk Naval Shipyard; Mr. Bob Brunner of Long Beach Naval Shipyard; Mr. Barry Brinson of Charleston...Management, McGraw-Hill, 1978. Pressman , Roger S.. Software Engineering: A Practitioner’s Approach, McGraw-Hill, 1982. Project Systems Consultants Inc...J.H. Shoemaker Code 377 Norfolk Naval Shipyard Portsmouth. Vireinih 23709-5000 13. Mr. Barry Brinson-I Code 377 Charleston Naval Shipyard Charleston

  9. Theoretical Analysis of Microwave Propagation.

    DTIC Science & Technology

    1984-04-01

    effects of the turbulent atmosphere on wave propagation, US Department of Commerce, NTIS, Springfield, VA. * VanVleck, J.H. (1947), "The absorption of... turbulent atmosphere on wave propagation, US Department of Commerce, NTIS, Sprinifield, VA. -. A. Vigants (1975), "Space diversity engineering," BSTJ, Vol... Turbulence ., ...... 2-26 2.5 ATMOSPHERIC ABSORPTION. 2-26 2 .6 RAIN ATTENUATION ....... 2-29 3 TROPOSCATTER PROPAGATION .................... 3-1 3.1

  10. First Time Authentication for Airborne Networks (FAAN)

    DTIC Science & Technology

    2010-01-01

    21] “An Improved Protocol for Demonstrating Possession of Discrete Logarithms and Some Generalizations”, D Chaum , J-H Evertse, J van de Graaf...System Sciences, p. 1-9, 1998. [5] D . Micciancio, The Shortest Vector in a Lattice is Hard to Approximate to within Some Constant, Proc. 39th...1999. [7] D . Micciancio and E. Petrank, Efficient and Concurrent Zero-Knowledge from any public coin HVZK protocol, Proceedings of Advances in

  11. Modeling Respiratory Gas Dynamics in the Aviator’s Breathing System. Volume 2. Appendices

    DTIC Science & Technology

    1994-05-01

    Rideout, at at. Dfference-Differentlat Equations for Fluid C... Flow in Distensible Tubes. IEEE Transactions on Bio-Medlcat C... Enginhering. Vot INE-14...McGraw-Hill; 1970; Chapter 13: 433-450. 12. Astrand, PO; Saltin, B. Oxygen uptake during the first minutes of heavy muscular exercise. J Appl Physiol...1802-1814; 1986. 233. Linehan, JH; Haworth, ST; Nelin, LD; Krenz, GS; Dawson, CA. A Simple Distensible Vessel Model for Interpreting Pulmonary

  12. Bode's Astronomisches Jahrbuch as an international archive journal. (German Title: Bodes Astronomisches Jahrbuch als internationales Archivjournal)

    NASA Astrophysics Data System (ADS)

    Kokott, Wolfgang

    The Astronomisches Jahrbuch, published from 1776 onwards by the Royal Academy of Sciences at Berlin, was to include ``a collection of the most recent observations, news, remarks and contributions''. Established by J.H. Lambert and edited for five decades by J.E. Bode, this almanac became from the start a high ranking international publication, with Bode's modest Berlin Observatory serving as a clearinghouse of information originating from virtually all European countries.

  13. Roles of germline JAK2 activation mutation JAK2 V625F in the pathology of myeloproliferative neoplasms.

    PubMed

    Wu, Qing-Yun; Ma, Meng-Meng; Fu, Lin; Zhu, Yuan-Yuan; Liu, Yang; Cao, Jiang; Zhou, Ping; Li, Zhen-Yu; Zeng, Ling-Yu; Li, Feng; Wang, Xiao-Yun; Xu, Kai-Lin

    2018-05-18

    Janus tyrosine kinase 2 (JAK2) mediates downstream signaling of cytokine receptors in all hematological lineages, constitutively active somatic JAK2 mutations play key roles in the pathology of myeloproliferative neoplasms (MPNs). Recently, germline JAK2 mutations are also associated with triple-negative MPNs. A novel germline mutation JAK2 V625F is reported to be involved in a subset of MPNs patients. However, the pathogenesis of this mutation caused MPN is still unclear. In this study, the homology models of JAK2 V625F showed that the newly formed interaction between F625 and Y613 disrupted the JAK2 JH1-JH2 domain interactions was responsible for its activation, when F625 and Y613 interaction was disrupted, its activity significantly decreased. While, when this interaction was repaired whether by forming hydrogen bond or salt bond, it would cause JAK2 activation. Biochemical studies also demonstrated that JAK2 V625F mutation led to JAK2-STAT5 pathway activation and promoted the proliferation of BaF3 cells. Thus, our results herein provide clues to understand the mechanism JAK2 V625F mutation caused MPNs and give information for the development of JAK2 mutation specific inhibitors. Copyright © 2018 Elsevier B.V. All rights reserved.

  14. Identifying artificial selection signals in the chicken genome.

    PubMed

    Ma, Yunlong; Gu, Lantao; Yang, Liubin; Sun, Chenghao; Xie, Shengsong; Fang, Chengchi; Gong, Yangzhang; Li, Shijun

    2018-01-01

    Identifying the signals of artificial selection can contribute to further shaping economically important traits. Here, a chicken 600k SNP-array was employed to detect the signals of artificial selection using 331 individuals from 9 breeds, including Jingfen (JF), Jinghong (JH), Araucanas (AR), White Leghorn (WL), Pekin-Bantam (PB), Shamo (SH), Gallus-Gallus-Spadiceus (GA), Rheinlander (RH) and Vorwerkhuhn (VO). Per the population genetic structure, 9 breeds were combined into 5 breed-pools, and a 'two-step' strategy was used to reveal the signals of artificial selection. GA, which has little artificial selection, was defined as the reference population, and a total of 204, 155, 305 and 323 potential artificial selection signals were identified in AR_VO, PB, RH_WL and JH_JF, respectively. We also found signals derived from standing and de-novo genetic variations have contributed to adaptive evolution during artificial selection. Further enrichment analysis suggests that the genomic regions of artificial selection signals harbour genes, including THSR, PTHLH and PMCH, responsible for economic traits, such as fertility, growth and immunization. Overall, this study found a series of genes that contribute to the improvement of chicken breeds and revealed the genetic mechanisms of adaptive evolution, which can be used as fundamental information in future chicken functional genomics study.

  15. An optimal stratified Simon two-stage design.

    PubMed

    Parashar, Deepak; Bowden, Jack; Starr, Colin; Wernisch, Lorenz; Mander, Adrian

    2016-07-01

    In Phase II oncology trials, therapies are increasingly being evaluated for their effectiveness in specific populations of interest. Such targeted trials require designs that allow for stratification based on the participants' molecular characterisation. A targeted design proposed by Jones and Holmgren (JH) Jones CL, Holmgren E: 'An adaptive Simon two-stage design for phase 2 studies of targeted therapies', Contemporary Clinical Trials 28 (2007) 654-661.determines whether a drug only has activity in a disease sub-population or in the wider disease population. Their adaptive design uses results from a single interim analysis to decide whether to enrich the study population with a subgroup or not; it is based on two parallel Simon two-stage designs. We study the JH design in detail and extend it by providing a few alternative ways to control the familywise error rate, in the weak sense as well as the strong sense. We also introduce a novel optimal design by minimising the expected sample size. Our extended design contributes to the much needed framework for conducting Phase II trials in stratified medicine. © 2016 The Authors Pharmaceutical Statistics Published by John Wiley & Sons Ltd. © 2016 The Authors Pharmaceutical Statistics Published by John Wiley & Sons Ltd.

  16. Brain gene expression changes elicited by peripheral vitellogenin knockdown in the honey bee.

    PubMed

    Wheeler, M M; Ament, S A; Rodriguez-Zas, S L; Robinson, G E

    2013-10-01

    Vitellogenin (Vg) is best known as a yolk protein precursor. Vg also functions to regulate behavioural maturation in adult honey bee workers, but the underlying molecular mechanisms by which it exerts this novel effect are largely unknown. We used abdominal vitellogenin (vg) knockdown with RNA interference (RNAi) and brain transcriptomic profiling to gain insights into how Vg influences honey bee behavioural maturation. We found that vg knockdown caused extensive gene expression changes in the bee brain, with much of this transcriptional response involving changes in central biological functions such as energy metabolism. vg knockdown targeted many of the same genes that show natural, maturation-related differences, but the direction of change for the genes in these two contrasts was not correlated. By contrast, vg knockdown targeted many of the same genes that are regulated by juvenile hormone (JH) and there was a significant correlation for the direction of change for the genes in these two contrasts. These results indicate that the tight coregulatory relationship that exists between JH and Vg in the regulation of honey bee behavioural maturation is manifest at the genomic level and suggest that these two physiological factors act through common pathways to regulate brain gene expression and behaviour. © 2013 Royal Entomological Society.

  17. National Program for Inspection of Non-Federal Dams. Muddy cove Pond Dam (MA 00793) Taunton River Basin, Dighton, Massachusetts. Phase I Inspection Report.

    DTIC Science & Technology

    1979-11-01

    Oinerra bra msoe Frew T..Tretion................. at. C*rpw ftA Tel Jh 5*1tut __ _ 3 ea at. a the atypon sto TV. T’. CA tya. rs (if mw) e.g...X APPENDIX D)-7 civ .~ CUiMfl co1mI dor FW05 JSO30621 PAGE POJECT M z൒ DATE CHECKED~ ± GLMI--t ATE OSAIL fMD = dopvfCHCDBY1F -A4 *y ourvsrawm_,F -W

  18. Patterns of Care and Disparities in the Treatment of Early Breast Cancer

    DTIC Science & Technology

    2008-09-30

    renal impairment in heart failure : mortality in blacks versus whites. Circulation. 2005 Mar 15;111(10):1270-7. 19. Smith BD, Smith GL, Haffty BG...Lichtman JH, Krumholz HM. Functional status and quality of life in heart failure patients with renal impairment. Circulation 106(16):e76, 2002...Theses 1. Ph.D.: Smith GL. Renal Impairment in Heart Failure : Prevalence, Prognosis, and Detection. 2007. 41 2. M.D.: Smith GL. Patterns of

  19. Mechanisms of Angiogenesis and Lymphangiogenesis in TSC Skin Tumors

    DTIC Science & Technology

    2011-09-16

    associated macrophage PKD Polycystic kidney disease TGF-β Transforming growth factor-β pS6 Phospho ribosomal protein S6 TNF-α Tumor necrosis factor...blood supply. They are found in the skin and other tissues except the central nervous system, bone marrow and avascular structures including epidermis...273:8413-8418 232. Cha HS, Bae EK, Koh JH, Chai JY, Jeon CH, Ahn KS, Kim J, Koh EM: Tumor necrosis factor-alpha induces vascular endothelial

  20. Analysis of Low-Frequency Unsteadiness in Shock and Turbulent Boundary Layer Interactions Using Direct Numerical Simulation Data

    DTIC Science & Technology

    2011-02-09

    1991; Beresh , Clemens, & Dolling, 2002; Ganapathisubramani, Clemens & Dolling, 2007) and (b) the downstream separated flow (Thomas, Putnam & Chu...Ferziger, J.H., Reynolds, W.C. (1980) “Improved subgrid-scale models for large eddy simulation,” AIAA Paper 1980-1357. 5. Beresh , S.J., Clemens, N.T...AIAA Journal 25, 5, 668-675. 34. Lempert, W.R., Wu, and Miles, R.B. (1997) “Filtered Rayleigh scattering measurements using a MHz rate pulse

  1. Asymptotic Description of Damping Mistuning Effects on the Forced Response of Turbomachinery Bladed Disks

    DTIC Science & Technology

    2012-07-31

    damping produced at friction and interface joints. For example, in the case of the turbine wheels produced for turbochargers for the automotive industry...effect of damping mistuning. The AMM can be regarded as an extension of the Fundamental Mistuning Model (FMM) [8, 9, 10] for the very frequent...blisks. presented at the ASME Turbo Expo, Vancouver, Canada, 2011, GT2011- 45611, 2011. [8] D.M. Feiner and J.H. Griffin. A fundamental model of mistuning

  2. Multidisciplinary Analysis of Cyclophilin A Function in Human Breast Cancer

    DTIC Science & Technology

    2010-03-01

    between prolyl isomerases and cell surface receptors. Figure 1. Regulation of the prolactin receptor/Janus kinase 2 (PRLr...334 in the X-box motif of the PRLr; the red circle containing P indicates phosphorylation of JAK2 kinase , PRLr, and signal transducer and activator of...shares seven significant Janus homology domains (JH1-7) (from the C- to the N-terminus) to other members of the Jak kinase family as described below: (1

  3. Brain and Plasma Molecular Characterization of the Pathogenic TBI-AD Interrelationship in Mouse Models

    DTIC Science & Technology

    2015-10-01

    collegiate football players: the NCAA Concussion Study. JAMA 290, 2549-2555. Hinkebein, J.H., Martin, T.A., Callahan, C.D., and Johnstone, B. (2003). Concept...al., 2014). We have also developed a novel mouse model of mild TBI (mTBI)/ concussion in which we have demonstrated cognitive dysfunction at 6, 12...2010). Boxing-acute complications and late sequelae: from concussion to dementia. Dtsch Arztebl Int 107, 835-839. Gaetz, M., and Weinberg, H

  4. An Advanced Computational Approach to System of Systems Analysis & Architecting Using Agent-Based Behavioral Model: Phase 2

    DTIC Science & Technology

    2013-11-18

    for each valid interface between the systems. The factor is proportional to the count of feasible interfaces in the meta-architecture framework... proportional to the square root of the sector area being covered by each type of system, plus some time for transmitting data to, and double checking by, the...22] J.-H. Ahn, "An Archietcture Description method for Acknowledged System of Systems based on Federated Architeture ," in Advanced Science and

  5. Mechanisms of Protective Immunogenicity of Microbial Vaccines of Military Medical Significance.

    DTIC Science & Technology

    1981-01-30

    serodiagnosis of Q fever. We have had the opportunity to test a large number of positive samples from recent laboratory associated infections by both...note a remarkable stability of this assay in the uninfected individuals. The infected individuals show a clear change in FIAX activity after 0 fever...1979. { 3002 Brody, J.P., J.H. Binkley, and S.A. Harding. Evaluation and comparison of two assays for detection of immunity to rubella infection

  6. European Conference on Organized Thin Films (4th) Held in Bangor on September 10 - 12, 1992

    DTIC Science & Technology

    1992-09-12

    Blodgett films from discotic liquid-crystalline compounds . V.V.Tsukruk, J.H. Wendorff, O.Karthaus, and H.Ringsdorf (Germany/Ukraine) DP2 Alternate...backbone and yield two different domain shapes observ, j in the LE/LC phase coexistence range: one compound exhibits a mostly hexagonal sh:.pe, since...No.: 91113 464.1- films with predetermined alternation of three or more;. /4/ Y. Lvov, 0. Decher arnd H. Mbhwald different compounds in a ABAB...CDCD

  7. Atypical Pupillary Light Reflex in Individuals With Autism

    DTIC Science & Technology

    2012-07-01

    services at the University of Missouri Thompson Center for  Autism   and  Neurodevelopmental  Disorders, an interdisciplinary academic medical center...Center for Autism & Neurodevelopment Disorders, University of Missouri, Columbia, MO 65201, USA. (e-mail: MilesJH@missouri.edu) S. E. Christ is with...Department of Radiology, Department of Neurology, Department of Psychological Sciences, and Thompson Center for Autism & Neurodevelopment Disorders

  8. A Computational Comparison of High Strain Rate Strength and Failure Models for Glass

    DTIC Science & Technology

    2012-11-05

    many researchers, however accuracy across a broad range of impact conditions is still not always achievable. Glasses , including soda - lime - silica ...plug/cone failure appearance when testing soda - lime - silica glass (see Fig. 5 from Ref. [7]). He notes that at 60 µs, the plug begins to break up and...material model. Although the JH-2 model has been adapated to provide reasonably accurate predictions for soda - lime glass , the Holmquist-Johnson model

  9. Defining Protein Electrostatic Recognition Processes

    DTIC Science & Technology

    1989-11-30

    of the electrostatic potentiai on the molecular surface of negatively charged Asp-101 in the fifth residue of JH1. the hapten and the V regions of...making and aligning expanded molecular dot surfaces for each molecule and checking these surfaces for interpenetration. The program TURNIP used these...the molecular surfaces are separated by 6 and 12A. All orientations have the exposed heme edge of cytochrome c facing the acidic patch of plastocyanin

  10. Development of Magnetic Resonance Imaging Biomarkers for Traumatic Brain Injury

    DTIC Science & Technology

    2012-07-01

    months) stages after injury, the patients will be brought back to repeat both the MRI scans and neurocognitive evaluations. Age/gender/ education ...J.H., 2010. On the contribution of deoxy- hemoglobin to MRI gray-white matter phase contrast at high field. Neuro- image 49, 193–198. Li, C., Langham...provides physical and structural support of neuronal and glial cells. From a physiological point of view, the blood flow provides nutritional sup- port

  11. Hybrid Molecule-Nanocrystal Photon Upconversion Across the Visible and Near-Infrared

    DTIC Science & Technology

    2015-07-10

    applications in solar energy, biological imaging , and data storage. In this Letter, CdSe and PbSe semiconductor nanocrystals are combined with molecular...Goldschmidt, J. C. Absolute Upconversion Quantum Yield of β-NaYF4 Doped with Er3+ and External Quantum Efficiency of Upconverter Solar Cell Devices...C. Peak External Photocurrent Quantum Efficiency Exceeding 100% via MEG in a Quantum Dot Solar Cell . Science 2011, 334, 1530−1533. (37) Choi, J.-H

  12. A Dynamic Model for Induced Reactivation of Latent Virus

    DTIC Science & Technology

    2005-12-04

    with human herpesvirus-6 in EBV -negative infectious mononucleosis , Journal of Medical Virology 71 (4), 569-577, 2003. [35] Wu FY, Ahn JH, Alcendor DJ...model that describes the reactivation of latent herpes virus by metabolic end products of anaerobic bacteria. Herpes viruses are double-stranded DNA ...cells that may be different from the types of cells targeted for primary infection. Latent virus persists in the cell nucleus as episomal DNA until it

  13. Molecular Mechanisms of Prostate Cancer Progression

    DTIC Science & Technology

    2005-01-01

    Beijersbergen RL, Knoll JH, Meyerson M, Weinberg RA (1999) Inhibition of telomerase limits the growth of human cancer cells. Nat Med 5:1164-70. Hayflick ...nontumorigenic cells and show an increase in p23 without a concomitant increase in telomerase activity, suggesting that p23 is not limiting in these cells...without an increase in assembly as chaperones are limiting . Interestingly, we observe a significant increase in activity after hTERT expression (see

  14. Cerebral Acetylcholine and Choline Contents and Turnover Following Low-Dose Acetylcholinesterase Inhibitor Treatment in Rats

    DTIC Science & Technology

    2006-05-01

    JH, Jr., Romano JA, King JM (1990) Age-related differences in soman toxicity and in blood and brain regional cholinesterase activity . Brain Res.Bull...of OP AChE inhibitors when given in anticipation of exposure to toxic nerve agents. The mechanism of this protection seems to be the pre- occupation of...has indicated effects on blood AChE activity during and shortly after treatment and delayed effects, 2 to 16 weeks post-treatment, on exploratory

  15. PSA-Based Screening Outcomes, Dietary Heterocyclic Amine Exposure, and Prostate Cancer Risk in African-Americans

    DTIC Science & Technology

    2006-01-01

    associated micronutrients , and risk of prostate cancer. Epidemiol. Rev. 2001a: 23: 82-86. Chan J., and Giovannucci E. Dairy products, calcium, and...118. Chan J., and Giovannucci E. Vegetables, fruits, associated micronutrients , and risk of prostate cancer. Epidemiol. Rev. 2001a: 23: 82-86...Chan J., and Giovannucci E. Dairy products, calcium, and vitamin D, and risk of prostate cancer. Epidemiol. Rev. 2001b: 23: 87-92. Cohen J.H., Kristal

  16. Physical Property Data Review of Selected Chemical Agents and Related Compounds: Updating Field Manual 3-9 (FM 3-9)

    DTIC Science & Technology

    2003-09-01

    Ed.; Reinhold Book Corporation: New York, 1968. Daroff, P.M.; Metz, D.; Roberts, A.; Adams, J.A.; Jenkins, W. Oleoresin Capsicum : An Effective Less...Capsaicin, The Pungent Principle of Capsicum . III. J.Am. Chem. Soc. 1923, 45, p 2179. Newman, J.H., Edgewood Arsenal Notebook # NB 9298, p 64 (U...Service: Edgewood Arsenal, MD, 1921, UNCLASSIFIED Report (AD-B955153). Steadman, A. Isolation ofCapsaicin from Capsicum ; EACD 188; U.S. Army Chemical

  17. Thermal Runaway Due to Strain-Heading Feedback,

    DTIC Science & Technology

    1985-05-28

    ApprovedREPORT DOCUMENTATION PAGE OMBNo. 0704-0188 la. REPORT SECURITY CLASSIFICATION lb. RESTRICTIVE MARKINGS 2a. SECURITY CLASSIFICATION AUTHORITY 3...instability. The thermal runaway phenomenon has been discussed in the geophysics literature (e.g. Brun and Cobbold 1980, Cary et al. 1979 and Wan et al...pp. 325-342. Boley, B.A. and Weiner, J.H., 1960, Theory of Thermal Stresses, John Wiley and Sons, Inc. Brun, Y.P. and Cobbold , P.R., 1980, Strain

  18. A Meta-Analysis of the Correlates of Role Conflict and Ambiguity.

    DTIC Science & Technology

    1982-05-01

    Lyons , 1971; Stead and Scamell, 198; Ivancevich and Donnelly, 1974), tenure (Brief, Aldag, Van Sell, Melone,. 1979), higher order need strength (Beehr...JourDnal o _ ft is Psucholoau. 1965, 4 24-33. Lyons .. T.F. Role clarity, need for clarity, satisfaction, tension, * and withdrawal. Oranizational Behavior...82. Ivancevich , J.M. and Donnelly, J.H., Jr. A study of role clarity and need for clarity for three occupational groups. Academ af jt journal. 1974

  19. JPRS Report, Science & Technology, 16th International Congress of the International Society for Photogrammetry and Remote Sensing -- Volume 2

    DTIC Science & Technology

    1989-01-27

    Rego Barbosa, Osvaldo Caldas; MCT - MINISTERIO DA CIENCIA E TECNOLOGIA , INPE - INSTITUTO DE PESQUISAS ESPACIAIS, Rod. Presidente Dutra, km 40 12630...economic crisis tne country J.- xa^+ y recoanition from the governmental planners of the priorities ana importance of INPE’s role...87 8, 216 430 8, 646 The situation changed and in the last three years a clear -cove^is being observed. Not only Jh«* •=>«* fche u-er tenden y

  20. The Shock and Vibration Digest. Volume 14, Number 5

    DTIC Science & Technology

    1982-05-01

    Engrg., Santiago de Chile ,_[_, paper B3, pp 117- 129(1969). 13. Crandall, S.H., Lee, S.S., and Williams, J.H., Jr., "Accumulated Slip of a...Temperature, en - vironment, cumulative damage, and shock loading factors can also contribute to Cf depending on the operating conditions of the...Cycle Fatigue," ASME Proc. Intl. Conf. Reliability, Stress Analy- sis Failure Prevention Methods Mech. Des ., pp 237-245 (1980). 18. Shure, J.R. and

  1. Addition of Epidermal Growth Factor Improves the Rate of Sulfur Mustard Wound Healing in an In Vitro Model

    DTIC Science & Technology

    2008-03-26

    the wound site.1 Inflammation begins within minutes after the injury. Neutrophils clear out contami- nating bacteria and release cytokines to activate...of an in vitro wound healing model for periodontal cells. J Periodontol. 2000;71:226–37. 27. Oates TW, Mumford JH, Carnes DL, Cochran DL...Characterization of proliferation and cellular wound fill in periodontal cells using an in vitro wound model. J Periodontol. 2001;72:324–30. 28. Chuang AH

  2. Discovery of Sound in the Sea 2013 Annual Report

    DTIC Science & Technology

    2013-09-30

    develop and maintain resources that address the long-term goal. The resources include a website (Figure 1), a tri-fold educational pamphlet (available in...on whale watches during the winter months. The DOSITS tri-fold brochure was translated to French for distribution at the 21st International...University of Rhode Island. (tri-fold pamphlet ) Vigness-Raposa, K.J., Scowcroft, G., Miller, J.H., and Ketten, D.R. 2012. Discovery of Sound in

  3. Characterization of a highly polymorphic region 5′ to JH in the human immunoglobulin heavy chain

    PubMed Central

    Silva, Alcino J.; Johnson, John P.; White, Raymond L.

    1987-01-01

    A cloned DNA segment 1.25 kilobases (kb) upstream from the joining segments of the human heavy chain immunoglobulin gene revealed extensive polymorphic variation at this locus, and the polymorphic pattern was stably transmitted to the next generation. Genomic restriction analysis showed that the polymorphism was caused by insertions/deletions within an MspI/BamHI fragment. Sequencing of one allele, 848 base pairs (bp) long, revealed eleven 50-base-pair tandem repeats. A second allele, 648 bp long, was cloned from a human genomic cosmid library, sequenced, and found to contain four fewer repeats than the first allele. A survey of 186 chromosomes from unrelated individuals of primarily northern European descent revealed at least six alleles. Images PMID:2884636

  4. Trust Management for Encounter-Based Routing in Delay Tolerant Networks

    DTIC Science & Technology

    2010-05-15

    8217 4 O-i 0.5 E 1.6 a 90% M 300 i / fc -vfc 0.8:0.2 « 2 1 IDS 6OO5 Oil (0, 2] mis D WS p/DS 0.5% Eo [12,24] hrs I/;.• [160,320 480] min. Below we...34 IEEE Infocom, Barcelona , Spain, Apr. 2006, pp. 1-11. [3] J.H. Cho, A. Swami and I.R. Chen, "Modeling and Analysis of Trust Management for Cognitive

  5. Screening for and Inheritance of Resistance to Barley Leaf Stripe (Drechslera graminea),

    DTIC Science & Technology

    1987-12-01

    JORGENSEN, J.H. (1986,. Field assessment of partial resistance to powdery mildew in spring barley . Euphytica 35, 233-243. KRISTIANSSON, B. and NILSSON, B...the Laevigatum powdery mildew resistance via ’Vada’ and ’Minerva’. This suggests this resistance to occur in many varieties descending from ’Vada...kept free from powdery mildew by spraying with Bayleton (25% triadimefon WP) both in the greenhouse and in the field. This fungicide does not affect the

  6. Back Pain and Endurance Training of Back Muscles: Justification for Further Study in Helicopter Pilots.

    DTIC Science & Technology

    1997-05-29

    osteopenic women. J Am Geriatr Soc 1996 Jul;44(7):756-762. 12. Chaffin DB. Human strength capability and low-back pain. J Occup Med 1974 Apr;16(4... geriatric hospital. Scand J Rehabil Med 1978;10(4):201-209. 16. Donchin M, Woolf O, Kaplan L, Floman Y. Secondary prevention of low-back pain. A...FR, Bendix T, Skov P, Jensen CV, Kristensen JH, Krohn L, Schoeler H. Intensive, dynamic back-muscle exercises, conventional physiotherapy , or

  7. DoD Space Radiation Concerns.

    DTIC Science & Technology

    1992-07-15

    cosmic - ray transport. NASA TM X-2440, 1972:117-122. DoD Space Radiation Concerns 8 2. Atkins SG, Small JT, McFarland TH. Military Man-in Space (MMIS...136. 29. Silberberg R, Tsao CH, Adams JH Jr., Letaw JR. Radiation doses and LET distributions of cosmic rays . Rad. Res., 1984, 98:209-226. 30. Stauber...levels on mission success and completion. Natural Radiation Trapped Radiation Belts Galactic Cosmic Rays (GCR) Solar Particle Events (SPEs) Man-Made

  8. Regression Models of Quarterly Indirect Labor Hours for NARF (Naval Air Rework Facility) Alameda.

    DTIC Science & Technology

    1987-09-01

    0 slim ~S74 88 .0 4x, Cy. be UU SN 89 z00 000 = S;JH 000 09 VI C.Cc z 0Y 0 CN Lam N~ 0 0000 009 ooo OG4 I-N C.91 pr- I- LU 001 00001 0002 * 0000t 000ge...1509 3286 7599 11345 9938 40906 16479 15204 13901 853 5888 1176 6975 12418 7970 41059 16078 13423 12030 854 7962 3699 9505 19587 10539 56100 25767 18647

  9. Psychiatric and Non-Psychiatric Predictors of Disability Discharge Disposition for Navy Personnel with a Mental Health Problem: A Replication and Extension

    DTIC Science & Technology

    1988-10-05

    Gottlieb, A. (1981). Attribution theory and symbolic interactionism : A comparison. In J.H. Howes, W. Ickes, & R.F. Kidd (Eds.), New directions in...ORGANIZATION REPORT NUMBER(S) NHRC Report No. 88-40 6a NAME OF PERFORMING ORGANIZATION 6b OFFICE SYMBOL 7a NAME OF MONITORING ORGANIZATION (If applicable) Naval...85122 Department of the Navy San Diego, CA 92138-9174 Washington, DC 20372 8a. NAME OF FUNDING/SPONSORING 8b OFFICE SYMBOL 9 PROCUREMENT INSTRUMENT

  10. Dugway Proving Ground: Closure Plan Module 2, for SWMUs 20, 164, 166 and 170

    DTIC Science & Technology

    1997-07-01

    Lewin and J.H. Holmes, 1972. Syndrome of Dyspraxia and Multifocal Seizure Associated with Chronic Hemodialysis. Trans. Am. Soc. Artif. Intern. Organs. 18...Many cases of nasal mucosal injury (inflamed mucosa, ulcerated or perforated septum) in workers exposed to Cr0 3 have been reported (Bloomfield and...mg/kg chromium for 3 or 6 weeks. These changes included neuronal degeneration in the cerebral cortex, marked O DUG\\0566 07/11/96 4:19pm bpw B.3-69

  11. Diet Restriction and Fasting Exacerbate the Toxicity of Soman in Young and Old Guinea Pigs

    DTIC Science & Technology

    2012-09-01

    1987;39:35-42. McDonough JH, Benjamin A, McMonagle JD, Rowland T, Shih TM. Effects of fosphenytoin on nerve agent-induced status epilepticus . Drug Chem...animals showed greater toxicity to soman than old animals. The notable exception was old DR-F animals. Guinea pigs of similar age and dietary status are...toxicity with overt toxicity. Alternatively, verifying the LD50 in a subset of animals with the same age, dietary restriction, and fasting status for

  12. Response of 40 and Over Aged Military Personnel to an Unsupervised, Self-Administered Aerobic Training Program,

    DTIC Science & Technology

    1982-01-06

    years of age and older . Personnel in this age group are almost exclusively senior staff personnel who often do not have the opportunity to train on a...1977. Physical fitness programs for adults In: Exercise in Cardiovascular Health and Disease. E.A. Amsterdam, J.H. Wilmore, and A.N. DeMaria, Eds...recently extended mandatory physical training and testing to in- clude personnel 40 yrs of age and older . The purpose of this study was to describe the

  13. Metallurgical Characterization of the Interfaces and the Damping Mechanisms in Metal Matrix Composites.

    DTIC Science & Technology

    1986-04-15

    Technical Reports - none IL.. Publications - none 11.g. Honors, Awards - none 11.h. Participants Mr. Asok Ray ; Graduate Student working towards his...Invited Presentation at Topical or Scientific/Technical Society Conferences i) A.K. Ray and V.K. Kinra, "Measurement of Damping in Continuous Fiber...Rawal, J.H. Armstrong, M.S. Misra, A.K. Ray and V.K. Kinra, "Damping Measurements of Gr/Al Composites", to be presented at the symposium on Dynamic

  14. Anatomical and Physiological Characteristics of the Ferret Lateral Rectus Muscle and Abducena Nucleus

    DTIC Science & Technology

    2007-01-25

    concerned with maintaining gaze control and the ability to acquire visual targets (36). A great deal has been written on the physiology of EOM in animal...borrows, the need for rapid nystagmus control is reduced. The ferret eyes are more laterally placed than either cats or monkeys which increases the visual...20. Hein A, Courjon JH, Flandrin JM and Arzi M. Optokinetic nystagmus in the ferret: including selected comparisons with the cat. Exp Brain Res 79

  15. Triterpenoidal Saponins: Bioactive Secondary Metabolites from Zygophyllum coccineum

    DTIC Science & Technology

    2011-01-22

    isolation of nine ursane-type triterpene saponins (1?9), including the new one; zygophylloside S (1), together with a known flavonoid glycoside (10) and a...1–9), including the new one; zygophylloside S (1), to- gether with a known flavonoid glycoside (10) and a sterol glyco- side (11).The isolated...Moghazy AM. Zygophyllum coccineum. V. The chemistry of the leaf and stem. J Pharm Sci 1960; 1: 135 4 Eskander EF, Won JH. Hypoglycaemic and

  16. Hormonal Interference with Pheromone Systems in Parasitic Acarines, Especially Ixodid Ticks.

    DTIC Science & Technology

    1985-05-01

    was found in mites and ticks, Studies done by Dr. Oliver and his colleagues demonstrated partial loss o reproductive capacity in Dermanyssus gallinae ...involved the use of the anti-JH compound precocene 2 (P2) applied to ,.. the chicken mite, Dermanyssus gallinae . Experiments were designed to deter...in acarines similarly as in insects. Two mg of P2 were exposed to fed female Dermanyssus gallinae for vari- ous lengths of time (24-96 h) and at

  17. Index to FAA Office of Aerospace Medicine Reports

    DTIC Science & Technology

    2011-01-01

    Development and evaluation of a radar air traffic control research task. AD660198 65-32 Gogel WC, Mertens HW: Problems in depth perception: A method of...and the air traffic control specialist: Some conclusions from a decade of research . ADA093266/5 80-15 Boone JO, Van Buskirk L, Steen JA: The Federal... control applicants. ADA328998 97-18 Marcus JH: A flexible cabin simulator. ADA328996 97-19 Broach D: Designing selection tests for the future National

  18. Insurgency and Counterinsurgency in Central America

    DTIC Science & Technology

    1983-06-01

    broadly based. In Guatemala, the revolution is at a different stage, and in Honduras, it is an embryo , although the condition? "üJ" ’"MwMfc Jh...who declared "The people of this continent alone have, the right to decide their own destiny ," and by President Theodore Roosevelt in 1904 when he...becoming an embryo government-in-exile. Although in many respects an international propaganda organization, the FDR does have one Important body--the

  19. Transmission Electron Microscopy (TEM) Observations of Female Oocytes From Nilaparvata lugens (Hemiptera: Delphacidae): Antibiotic Jinggangmycin (JGM)-Induced Stimulation of Reproduction and Associated Changes in Hormone Levels.

    PubMed

    Xu, Bing; You, Lin-Lin; Wu, You; Ding, Jun; Ge, Lin-Quan; Wu, Jin-Cai

    2016-08-01

    Previous studies have demonstrated that the agricultural antibiotic jinggangmycin (JGM) stimulates reproduction in the brown planthopper Nilaparvata lugens Stål and that the stimulation of brown planthopper reproduction induced by JGM is regulated by the fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) genes. However, a key issue in the stimulation of reproduction induced by pesticides involves the growth and development of oocytes. Therefore, the present study investigated oocyte changes via transmission electron microscopy (TEM) and changes in hormone levels (juvenile hormones (JH) and 20-hydroxyecdysone (20 E)) in JGM-treated females. TEM observations showed that the size of the lipid droplets in the oocytes of JGM-treated females, compared with those in the oocytes of the control females, significantly reduced by 32.6 and 29.8% at 1 and 2 d after emergence (1 and 2 DAE), respectively. In addition, the JH levels of JGM-treated females at 1 and 2 DAE were increased by 49.7 and 45.7%, respectively, whereas 20 E levels decreased by 36.0 and 30.0%, respectively. We conclude that JGM treatments lead to substantial changes in lipid metabolism, which are directly and indirectly related to stimulation of reproduction of brown planthopper together with our previous findings. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America.

  20. Hormone response to bidirectional selection on social behavior.

    PubMed

    Amdam, Gro V; Page, Robert E; Fondrk, M Kim; Brent, Colin S

    2010-01-01

    Behavior is a quantitative trait determined by multiple genes. Some of these genes may have effects from early development and onward by influencing hormonal systems that are active during different life-stages leading to complex associations, or suites, of traits. Honey bees (Apis mellifera) have been used extensively in experiments on the genetic and hormonal control of complex social behavior, but the relationships between their early developmental processes and adult behavioral variation are not well understood. Bidirectional selective breeding on social food-storage behavior produced two honey bee strains, each with several sublines, that differ in an associated suite of anatomical, physiological, and behavioral traits found in unselected wild type bees. Using these genotypes, we document strain-specific changes during larval, pupal, and early adult life-stages for the central insect hormones juvenile hormone (JH) and ecdysteroids. Strain differences correlate with variation in female reproductive anatomy (ovary size), which can be influenced by JH during development, and with secretion rates of ecdysteroid from the ovaries of adults. Ovary size was previously assigned to the suite of traits of honey bee food-storage behavior. Our findings support that bidirectional selection on honey bee social behavior acted on pleiotropic gene networks. These networks may bias a bee's adult phenotype by endocrine effects on early developmental processes that regulate variation in reproductive traits. © 2010 Wiley Periodicals, Inc.

  1. Fragrance material review on 16-hydroxy-7-hexadecenoic acid lactone.

    PubMed

    McGinty, D; Letizia, C S; Api, A M

    2011-12-01

    A toxicologic and dermatologic review of 16-hydroxy-7-hexadecenoic acid lactone when used as a fragrance ingredient is presented. 16-Hydroxy-7-hexadecenoic acid lactone is a member of the fragrance structural group macrocyclic lactone and lactide derivatives. The fragrance ingredient described herein is one of 12 structurally diverse C14, C15 and C16 compounds that include (1) saturated mono-and (2) saturated di-ester lactones and (3) unsaturated lactones. For the latter, the double bond is not adjacent to (in conjugation with) the ester group. This review contains a detailed summary of all available toxicology and dermatology papers that are related to 16-hydroxy-7-hexadecenoic acid lactone and is not intended as a stand-alone document. Available data were evaluated, then summarized, and include physical properties data. A safety assessment of the entire macrocyclic lactone and lactide derivatives will be published simultaneously with this document. Please refer to Belsito et al., 2011 for an overall assessment of the safe use of this material and all macrocyclic lactone and lactide derivatives in fragrances. Belsito, D., Bickers, D., Bruze, M., Calow, P., Dagli, M., Fryer, A.D., Greim, H., Hanifin, J.H., Miyachi, Y., Saurat, J.H., Sipes, I.G., 2011. A toxicologic and dermatologic assessment of macrocylic lactones and lactide derivatives when used as fragrance ingredients. Copyright © 2011. Published by Elsevier Ltd.

  2. Effects of tea saponin on growth and development, nutritional indicators, and hormone titers in diamondback moths feeding on different host plant species.

    PubMed

    Cai, Hongjiao; Bai, Yan; Wei, Hui; Lin, Shuo; Chen, Yixin; Tian, Houjun; Gu, Xiaojun; Murugan, Kadarkarai

    2016-07-01

    The diamondback moth (DBM) is an important worldwide pest. This insect has been studied for several decades; however, its control remains problematic. Numerous chemical insecticides have become ineffective and chemical residues constitute an important problem. In the present study, we determined the mortality of 3rd instar DBM larvae feeding on three different host plant species and exposed to various concentrations of tea saponin (TS). In addition, we evaluated growth and development parameters, nutritional indicators, and juvenile hormone (JH) and molting hormone (MH) titers in 2nd instar larvae exposed to LC20 and LC50 doses of TS. We found that treatment of DBM larvae with LC20 and LC50 doses of TS led to lower growth rates, decreased feed consumption, reduced frass production, lower pupal weights, reduced percentage pupation, slower adult emergence percentages, and diminished fecundity, but prolonged durations of the larval and pupal periods. The efficiency of conversion of ingested and digested food increased, but the approximate digestibility did not differ significantly between treatments and controls. JH and MH titers were higher after TS treatment; these increases varied according to the host species and TS concentration. Our results indicate that TS represents a potential new alternative insecticide based on its natural origin, low cost, and minimum environmental impact. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Reliability of Multilayer Copper/Polyimide

    DTIC Science & Technology

    1993-11-01

    LOIS H. WALSH R eliability R esearch E ng ineer -, . .....................-------- - - Reliability Physics Branch i-1’ , ý, Ay cci~i• es "AL f, , or Dist...average of 925 hours The chamber "as operated at 85 C󈨙 10 r h All the samples showed initial dielectric constant values consistent w%,ith previous...19. Matsumoto, 0., Katagiri, T., Thin Solid Films, 146, 283 (1987) 20. Malladi , D.P., J Membrane Sci., 19, 209 (1984) 21. Schubert, P.J., Nevin, J.H

  4. A Search for Gene Fusions/Translocations in Breast Cancer

    DTIC Science & Technology

    2013-11-01

    Ramnarayanan K, Brenner JC, Yu J , Kim JH, Han B, Tan P, Kumar-Sinha C, Lonigro RJ, Palanisamy N, Maher CA, Chinnaiyan AM. Science. 2008 Dec 12;322...Barrette TR, Grasso C, Yu J , Lonigro RJ, Schroth G, Kumar-Sinha C, Chinnaiyan AM. Proc Natl Acad Sci U S A. 2009 Jul 10. [Epub ahead of print]. PMID...CA, Palanisamy N, Mehra R, Kominsky HD, Siddiqui J , Yu J , Qin ZS, Chinnaiyan AM. Deep sequencing reveals distinct patterns of DNA methylation in

  5. Thermal Relaxation Processes and Stability in Poled Electro-Optic Polymers

    DTIC Science & Technology

    1994-06-30

    34, Gordon Research Conference on Dielectric Phenomena, Holderness School, NH July 31-August 5, 1994. 2. K.D. Singer, R. Dureiko, J. Khaydarov , and R...Fuerst, "Relaxation in Poled Electro- optic Polymers", 4th Iketani Conference, Hawaii, May 17-20, 1994. 3. J.H. Andrews, J.D.V. Khaydarov , and K.D. Singer...Dureiko, J. Khaydarov , and R. Fuerst, "Relaxation Phenomena in Poled Electro-Optic Polymers", Proc. Mat. Res. Soc. 328, 499 (1994). 5. R.A. Fuerst, "Thermal

  6. Toward Active Control of Noise from Hot Supersonic Jets

    DTIC Science & Technology

    2014-04-21

    regions of the jet. A retro -reflective shadowgraph setup was used to record the images. The near-nozzle region exhibits a large number of shock-like...jet exit plane; nearly identical observations have been made in the rocket noise community [15, 29| . The only discrepancies in figure 9b are with the...noise surveys of solid-fuel rocket engines for a range of nozzle exit pressures," NASA TN D-21, August, 1959. [16] Potter, R.C. and Jones, J.H., "An

  7. Pharmacological Sparing of Protein in Burn Injury

    DTIC Science & Technology

    1989-05-01

    skeletal muscle. Diabetes 27:1065-1074, 1978. 7. Gup, F.E., C.L. Long, J.W. Geiger, J.H. Kinney. The significance of altered gluconeogenesis in surgical...humans. Am. J. Physiol. 251(Endocrinol. Metab. 14):E334-E342, 1986. 30. Unger, R.H. Glucagon and insulin: Glucagn ratio in diabetes and other catabolic...illnesses. Diabetes 20:834-838, 1971. 31. Wolfe, R.R., D.N. Herndon, E.J. Peters, F. Jahoor, M.H. Desai, O.B. Holland. Regulation of lipolysis in

  8. Effect of Bioaerosol on the Release of Iodine from Iodine Treated Filters

    DTIC Science & Technology

    2011-12-01

    bacteriophage aerosols”, Journal of Applied Microbiology , 107, 1912–1923. (7) Prescott , L.M., Harley, J.P. and Klein, D.A. (2002). Microbiology . 5th Ed... Prescott et al., 2002; Golmohammadi et al., 1993). MS2 is hydrophilic and negatively charged (Valegård et al, 1990). 2.2. Experimental system Figure 1...and ultrafine virus aerosol particles. Journal of Applied Microbiology , 99, 1422-1434. (6) Lee, J.H., Wu, C.Y., Lee, C.N., Anwar, D., Wysocki, K.M

  9. Developing a Zebrafish Model of NF1 for Structure-Function Analysis and Identification of Modifier Genes

    DTIC Science & Technology

    2012-04-01

    nuclease (ZFN) technology (Figure 34). We were fortunate to have the opportunity to collaborate closely with the laboratories Nathan Lawson and Scot Wolfe...controls tumour cell growth. Nature 441, 424–430. 32. Postlethwait, J.H., Woods , I.G., Ngo-Hazelett, P., Yan, Y.L., Kelly, P.D., Chu, F., Huang, H...Michael Granato2, Nathan D. Lawson4, Scot A. Wolfe4, Seok-Hyung Kim5, Lilianna Solnica- Krezel6, John P. Kanki1, Keith L. Ligon3, Jonathan A. Epstein2

  10. 50th Anniversary Celebration: 46th Sagamore Army Materials Research Conference on Advances and Needs in Multi-Spectral Transparent Materials Technology

    DTIC Science & Technology

    2008-09-01

    68 (4), 883–887. Li, C-W.; Yamanishi, J. Ceram. Eng. Sci. Proc. 1989, 10, 632–645. Li, J.-G.; Ikegami , T .; Lee, J.-H.; Mori, T .; J. Am. Ceram. Soc...and Opening Remarks Dr. Allen Grum Associate Director for Science and Technology (S& T ), ARL The Impact of Research on Soldier Protection Ms. Jill...Crystal Systems, Dr. Chandra Khattak 3. MSI, Dr. Les Bowen 4. TA& T , Dr. Larry Fehrenbacher 5. CeraNova, Dr. Marina Pascucci Session IV

  11. New Approaches to Novel Organosilanes

    DTIC Science & Technology

    1987-12-01

    Dean Seidler Mr. Sean Ouyang PUBLICATIONS resulting from support through AFOSR 88-0060 56. "A Convenient, Naphthalene-Catalyzed Synthesis of Alkali...and P. Boudjouk, Synthesi , (1989) 306. 91 8 0 6 2 03 Approve 6 ~or p,.o r aleese; dis5t r Ibut 0. u" 61. "Inorganic and Organometallic Synthesis with...34Dehydration of Metal Hydrates with Trimethylchlorosilane. A Simple and Convenient Route to Anhydrous Complexes ." P. Boudjouk and J.-H. So, Inorg. Chem

  12. Combat Automation for Airborne Weapon Systems: Man/Machine Interface Trends and Technologies (L’Automatisation du Combat Aerien: Tendances et Technologies pour l’Interface Homme/Machine)

    DTIC Science & Technology

    1993-04-01

    Homme /Machine) Aocesion For ; 1 [ NTIS ’ D:i: Ü J-H CRA& l TAB 3...I’utilisateur. - Enfm, utilise avec le bouton droit de la souris, le poten- tiom&tre de temps 6coul6 permet de charger une alterna- tive dans le syst&me...a a a a rn£Q £ OB E o 15 l | I? ^©J&Mß) NATO ^ OTAN 7 RUE ANCELLE • 92200 NEUILLY-SÜR-SEINE DIFFUSION DES PUBLICATIONS FRANCE AGARD

  13. Why are [P(C6H5)4]+N3- and [As(C6H5)4]+N3- Ionic Salts and Sb(C6H5)4N3 and Bi(C6H5)4N3 are Covalent Solids? A Theoretical Study Provides an Unexpected Answer

    DTIC Science & Technology

    2011-03-04

    Research, the Defense Threat Reduction Agency, and the Department of Energy. DAD is indebted to the Robert Ramsay Endowment of the University of Alabama...Schmidt, M. W., Baldridge, K. K., Boatz, J.A., Elbert, S.T., Gordon , M.S., Jensen, J.H., Koseki, S., Matsunaga, N., Nguyen, K.A., Su, S., Windus, T.L...Dupuis, M., Montgomery, J.A.; J. Comput. Chem., 1993, 14, 1347-1363.; Gordon , M. S., Schmidt, M. W., pp. 1167-1189, in "Theory and Applications of

  14. Molecular Level Understanding of Electrocatalysis in High pH Environment

    DTIC Science & Technology

    2015-07-08

    consisting of alkali metal hydroxide doped PBI membrane with 2.0 mgPtRu cm-2 anode and 1.0 mgPt cm-2 loadings at the anode and cathode, respectively...Direct!ethanol!fuel!cells!using!an!anion! exchange!membrane.!J!Power!Sources.!2008;185:621*6.! [4]!Hou!H,!Sun!G,!He!R,!Wu!Z,!Sun!B.!Alkali! doped ...electrocatalysts! for!oxygen!reduction! derived!from!polyaniline,!iron,!and! cobalt .!Science!(Washington,!DC,!U!S).!2011;332:443*7.! [17]! Zagal! JH

  15. Vertical Alignment of Single-Walled Carbon Nanotubes on Nanostructure Fabricated by Atomic Force Microscope

    DTIC Science & Technology

    2007-02-16

    SWNT films by Langmuir - Blodgett methods,8 and chemical assembly of SWNTs on a large substrate.9 Al- though these methods provide a good way to control... Langmuir - Blodgett to Self-Assembly. Academic: New York, (1991). [10] Moon, J.H., Shin, J.W., Kim, S.Y., Park, J.W. Langmuir , 12, 4621, (1996...aligning CNTs in solu- tion by applying an electric field5 or a magnetic field,6 align- ing SWNTs by blending them with liquid crystal,7 assem- bling

  16. The Energy Cost and Heart Rate Response of Trained and Untrained Subjects Walking and Running in Shoes and Boots,

    DTIC Science & Technology

    1983-01-01

    CH, Morrison JF, Viljoen JH, Heyns Aj (1968) The influence of boot weight on the energy expenditure of men walking on a treadmill and climbing stairs ...speed (4.0 km "h). These data indicate that energy expenditure is increased by wearing boots. A large portion of this increase may be attributed to...both shoes and boots except at the slowest walking speed (4.0 km ° h-1 ). These data indicate that energy expenditure is increased by wearing boots

  17. Electronic GaAs-on-Silicon Material for Advanced High-Speed Optoelectronic Devices

    DTIC Science & Technology

    1991-01-01

    of the substrate in contact with the growth ambient. Ira is the surface energy of the nucleated crystallite for a crystallographic orientation ( hkl ). A...Liquid-Phase Epitaxy" Journal of Applied Physics 63,4 (15 February 1988) 1075 -1079. 41. S. Sakai, S.S. Chang, R.V. Ramaswamy, J-H. Kim, G. Radha- krishnan...and Ge-Si-Pb Ternary Phase Diagram," Journal of the Electrochemical Society 117,8 (August 1970) 1075 -1079. 72. N.A. Yukasheva, K.S. Zhuravlev, S.I

  18. The Archaeology of Coralville Lake, Iowa. Volume 3. Sample Survey (Interim Report 1).

    DTIC Science & Technology

    1985-03-01

    n t ho I aite 19 60 ’ s , Air ian Anderson di rectedl a se-r ies coI Li in a ner e I cd s c1. ooIs v It 1h s tude ntLs f r om the tini v-er’si ty of r...wash cap the surface (Figure 13). 7 0- ii NORTH PROFILE 13 JH 479- UNIT 2 SIL WY 3/ c(HISTOWiCAL ALLUVIUM) stl ~SILT WVITH MINk CLAY JOYN 3/3 SCALE I

  19. The Role of IL-17 in the Angiogenesis of Rheumatoid Arthritis

    DTIC Science & Technology

    2013-07-01

    model of contact dermatitis . Am. J. Pathol. 144: 244–259. 34. Shahrara, S., A. E. Proudfoot, J. M. Woods, J. H. Ruth, M. A. Amin, C. C. Park, C. S. Haas...vascular endothelium: up- regulation in an in vivo model of contact dermatitis . Am J Pathol 1994;144:244–59. 27. Haas CS, Amin MA, Ruth JH, Allen BL, Ahmed...recognizing a novel antigen expressed on aberrant vascular endothelium. Upregulation in an in vivo model of contact dermatitis . Am J Pathol 144:244

  20. Pharmacological Studies of NOP Receptor Agonists as Novel Analgesics

    DTIC Science & Technology

    2010-05-01

    hydroxymethyl-4-piperidyl]-3-ethyl-1, 3-dihydro- 2H- benzimidazol -2-one (J-113397). J Med Chem 42: 5061–5063. Ko MC, Butelman ER, Traynor JR, Woods JH (1998a...ethyl-1,3-dihydro-2H- benzimidazol -2-one) was used to compare their antagonist effects against both morphine (100 nmol)- and UFP-112 (10 nmol)-induced...3S,4S)-1- (Cyclooctylmethyl)-3-(hydroxymethyl)-4-piperidinyl]-3-ethyl- 1,3-dihydro-2H- benzimidazol -2-one) (Tocris Bioscience, Ellisville, MO), and