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1

Dong quai  

MedlinePLUS

... skin of the penis as part of a multi-ingredient preparation for treating premature ejaculation. In Southeast ... For early orgasm in men (premature ejaculation): a multi-ingredient cream preparation containing Panax ginseng root, dong ...

2

Senescence effects of Angelica sinensis polysaccharides on human acute myelogenous leukemia stem and progenitor cells.  

PubMed

Leukemia stem cells (LSCs) play important roles in leukemia initiation, progression and relapse, and thus represent a critical target for therapeutic intervention. Hence, it is extremely urgent to explore new therapeutic strategies directly targeting LSCs for acute myelogenous leukemia (AML) therapy. We show here that Angelica sinensis polysaccharide (ASP), a major active component in Dong quai (Chinese Angelica sinensis), effectively inhibited human AML CD34+CD38? cell proliferation in vitro culture in a dose-dependent manner while sparing normal hematopoietic stem and progenitor cells at physiologically achievable concentrations. Furthermore, ASP exerted cytotoxic effects on AML K562 cells, especially LSC-enriched CD34+CD38? cells. Colony formation assays further showed that ASP significantly suppressed the formation of colonies derived from AML CD34+CD38? cells but not those from normal CD34+CD38? cells. Examination of the underlying mechanisms revealed that ASP induced CD34+CD38? cell senescence, which was strongly associated with a series of characteristic events, including up-regulation of p53, p16, p21, and Rb genes and changes of related cell cycle regulation proteins P16, P21, cyclin E and CDK4, telomere end attrition as well as repression of telomerase activity. On the basis of these findings, we propose that ASP represents a potentially important agent for leukemia stem cell-targeted therapy. PMID:24377566

Liu, Jun; Xu, Chun-Yan; Cai, Shi-Zhong; Zhou, Yue; Li, Jing; Jiang, Rong; Wang, Ya-Ping

2013-01-01

3

Natural medicine: the genus Angelica.  

PubMed

More than 60 species of medicinal plants belong to the genus Angelica (Family: Apiaceae). Many of these species have long been used in ancient traditional medicine systems, especially in the far-east. Various herbal preparations containing Angelica species are available over-the-counter, not only in the far-eastern countries, but also in the western countries like USA, UK, Germany, etc. For centuries, many species of this genus, e.g. A. acutiloba, A. archangelica, A. atropupurea, A. dahurica, A. japonica, A. glauca, A. gigas, A. koreana, A. sinensis, A. sylvestris, etc., have been used traditionally as anti-inflammatory, diuretic, expectorant and diaphoretic, and remedy for colds, flu, influenza, hepatitis, arthritis, indigestion, coughs, chronic bronchitis, pleurisy, typhoid, headaches, wind, fever, colic, travel sickness, rheumatism, bacterial and fungal infections and diseases of the urinary organs. Active principles isolated from these plants mainly include various types of coumarins, acetylenic compounds, chalcones, sesquiterpenes and polysaccharides. This review evaluates the importance of the genus Angelica in relation to its traditional medicinal uses, alternative medicinal uses in the modern society and potential for drug development, and summarises results of various scientific studies on Angelica species or Angelica-containing preparations for their bioactivities including, antimicrobial, anticancer, antitumour, analgesic, anti-inflammatory, hepatoprotective, nephroprotective, etc. PMID:15180579

Sarker, S D; Nahar, L

2004-06-01

4

[A new phthalide from angelicae sinensis radix].  

PubMed

Angelicae Sinensis Radix, dried roots of Angelicae sinensis, is a well-known traditional Chinese medicine. Six compounds were isolated and purified by silica gel and preparative HPLC. According to physicochemical properties and spectral data, the compounds were identified as senkyunolide H-7-acetate (1), o-phthalic acid (2), diisobutyl phthalate (3), p-hydroxyphenylethanol ferulate (4), ferulic acid (5) and coniferylferulate (6). Compound 1 was a new one. PMID:24754173

Niu, Yan; Wang, Shu-Fang

2014-01-01

5

Ethnobotany of dye plants in Dong communities of China  

PubMed Central

Background Dyes derived from plants have an extensive history of use for coloring food and clothing in Dong communities and other indigenous areas in the uplands of China. In addition to use as coloring agents, Dong communities have historically utilized dye plants for their value for enhancing the nutritive, medicinal and preservative properties of foods. However, the persistence of plant-derived dyes and associated cultural practices and traditional knowledge is threatened with rapid socio-economic change in China. Research is needed to document the ethnobotany of dye plants in indigenous communities towards their conservation and potential commercialization as a sustainable means of supporting local development initiatives. Methods Semi-structured surveys on plants used for coloring agents and associated traditional knowledge were conducted in fifteen Dong villages of Tongdao County in Hunan Province of South Central China during 2011–2012. Transect walks were carried out with key informants identified from semi-structured surveys to collect samples and voucher specimens for each documented plant species for taxonomic identification. Results Dong households at the study sites utilize the flowers, bark, stems, tubers and roots of 13 plant species from 9 families as dyes to color their customary clothing and food. Out of the documented plants, a total of 7 are used for coloring food, 3 for coloring clothing and 3 for both food and clothing. Documented plants consist of 3 species that yield black pigments, 3 for brownish red/russet pigments, 3 for red pigments, 2 for dark blue pigments and 2 for yellow pigments. In addition to dyes, the plants have multiple uses including medicinal, ornamental, sacrificial, edible, and for timber. Conclusions The use of dyes derived from plants persists at the study sites for their important role in expressing Dong cultural identity through customary clothing and food. Further research is needed to evaluate the safety of dye plants, their efficacy in enhancing food items and their commercial potential. Conservation policies and management plans are called for to preserve these ethnobotanical resources in a sustainable manner that supports local livelihoods while maintaining cultural practices.

2014-01-01

6

Tyrosinase-inhibitory furanocoumarin from Angelica dahurica.  

PubMed

An extract of the roots of Angelica dahurica BENTH. et HOOK. (Umbelliferae) showed potential tyrosinase inhibition against mushroom tyrosinase. Employing a bioassay-linked HPLC method, followed by semipreparative HPLC, the active principle was isolated and characterized as 9-hydroxy-4-methoxypsoralen. PMID:15256758

Piao, Xiang Lan; Baek, Seung Hoon; Park, Man Ki; Park, Jeong Hill

2004-07-01

7

[Parasitic diseases in Dong-Eui-Bo-Kam].  

PubMed

Dong-Eui-Bo-Kam is a medical book of Korean traditional medicine, which is of encyclopedic characteristics. Its contents imply almost every field of medicine. There are also descriptions of human parasites in this book under the title of 'Worms'. Worms described in the book are Sam-Shi-Choong, Ku-Choong (nine worms) and Oh-Jang-Choong (worms of five organs). Among these Sam-Shi-Choong are not real worms, but quite mythological ones which have been mentioned in the religious Taoism. It is well-known that Dong-Eui-Bo-Kam has Taoistic characteristics. We can verify this characteristics by the fact that it mentions Sam-Shi-Choong on the top of the title 'Worms'. Dominant pathologic theory in the traditional medicine is a kind of balance theory, which defines the healthy state as the harmonious equilibrium of body elements. On the contrary parasitic diseases suppose real agents as the causes of diseases. This point of view appeares to be quite different from traditional pathologic view. PMID:11618931

Yeo, I S

1993-01-01

8

[Antimutagenic properties of Angelica archangelica L].  

PubMed

The antimutagenic activity of Angelica archangelica L. aqueous and alcohol extracts of thio-TEPA against mutagenicity was examined by the micronucleus test in murine bone marrow cells. The reduction of Thio-TEPA's mutagenic activity was more profound when the extracts were injected 2 hours before thio-TEPA treatment, as seen during simultaneous treatment. The observed reduction of micronuclear frequencies was as high as 77%. PMID:7767122

Salikhova, R A; Poroshenko, G G

1995-01-01

9

A fluorescence spectroscopy study of traditional Chinese medicine Angelica  

NASA Astrophysics Data System (ADS)

By measuring the fluorescence spectra of Chinese medicine (CM) Angelica water solutions with different concentrations from 0.025 to 2.5 mg/mL, results showed that the fluorescence intensity was proportional to the concentration. Through fluorescence spectra of Angelica solution under different pH values, results indicated coumarin compounds were the active ingredients of Angelica. We also observed fluorescence quenching of the Angelica solution in the presence of spherical silver nanoparticles with radius of 12 nm. Keeping a certain value for the volume of the silver nanoparticles, the fluorescence intensity at 402 nm was linearly proportional to the Angelica in the range of 1-3 mg/mL.

Zhao, Hongyan; Song, Feng; Liu, Shujing; Chen, Guiyang; Wei, Chen; Liu, Yanling; Liu, Jiadong

2013-10-01

10

[Furocoumarins in Archangelica officinalis and Angelica silvestris. Distribution of 5-beta-cyclolavandulyloxy-psoralen in the genus Angelica.].  

PubMed

5-beta-Cyclolavandulyloxy-psoralen i.e. 4-[(2,4,4-trimetnyl-1-cyclohexen-1-yl)methoxy]-7H-furo [3,2-g] [1] benzopyran-7-one was isolated from Angelica silvestris L. Detailed analytical data are given. Presence of this coumarin in other Angelica species is reported and discussed. PMID:17402103

Carbonnier, J; Molho, D

1982-03-01

11

Antiproliferative effect of Angelica archangelica fruits.  

PubMed

The aim of this work was to study the antiproliferative effect of a tincture from fruits of Angelica archangelica and the active components using the human pancreas cancer cell line PANC-1 as a model. Significant dose-dependent antiproliferative activity was observed in the tincture with an EC50 value of 28.6 microg/ml. Strong antiproliferative activity resulted from the two most abundant furanocoumarins in the tincture, imperatorin and xanthotoxin. The contribution of terpenes to this activity was insignificant. Imperatorin and xanthotoxin proved to be highly antiproliferative, with EC50 values of 2.7 microg/ml and 3.7 microg/ml, respectively, equivalent to 10 and 17 microM. The results indicate that furanocoumarins account for most of the antiproliferative activity of the tincture. PMID:15813373

Sigurdsson, Steinthor; Ogmundsdottir, Helga M; Gudbjarnason, Sigmundur

2004-01-01

12

Mucilaginibacter angelicae sp. nov., isolated from the rhizosphere of Angelica polymorpha Maxim.  

PubMed

A Gram-negative-staining, non-motile rod, designated GG-w14(T), was isolated from the rhizosphere of Angelica polymorpha Maxim. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolate belonged to the genus Mucilaginibacter and exhibited 93.9-97.4% 16S rRNA gene sequence similarity with recognized members of the genus Mucilaginibacter (closest relative Mucilaginibacter gossypii Gh-67(T)). DNA-DNA relatedness between strain GG-w14(T) and M. gossypii KCTC 22380(T) was <41%. Strain GG-w14(T) grew at 4-35 °C, at pH 5.0-8.0 and with 0-1% (w/v) NaCl. The isolate hydrolysed casein, CM-cellulose and starch and contained menaquinone 7 as the major menaquinone. The major cellular fatty acids were summed feature 3 (C(16:1)?7c and/or iso-C(15:0) 2-OH; 39.9%), iso-C(15:0) (24.2%) and iso-C(17:0) 3-OH (12.4%). The DNA G+C content was 42.5 mol%. These data suggest that strain GG-w14(T) should be considered as a representative of a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter angelicae sp. nov. is proposed. The type strain is GG-w14(T) (=KCTC 23250(T)=NCAIM B 02415(T)). PMID:21317281

Kim, Byung-Chun; Poo, Haryoung; Lee, Kang Hyun; Kim, Mi Na; Kwon, O-Yu; Shin, Kee-Sun

2012-01-01

13

Bioactivities of major constituents isolated from Angelica sinensis (Danggui)  

PubMed Central

Danggui, also known as Angelica sinensis (Oliv.) Diels (Apiaceae), has been used in Chinese medicine to treat menstrual disorders. Over 70 compounds have been isolated and identified from Danggui. The main chemical constituents of Angelica roots include ferulic acid, Z-ligustilide, butylidenephthalide and various polysaccharides. Among these compounds, ferulic acid exhibits many bioactivities especially anti-inflammatory and immunostimulatory effects; Z-ligustilide exerts anti-inflammatory, anti-cancer, neuroprotective and anti-hepatotoxic effects; n-butylidenephthalide exerts anti-inflammatory, anti-cancer and anti-cardiovascular effects.

2011-01-01

14

Pharmacological effects of Radix Angelica Sinensis (Danggui) on cerebral infarction  

PubMed Central

Radix Angelica Sinensis, the dried root of Angelica sinensis (Danggui), is a herb used in Chinese medicine to enrich blood, promote blood circulation and modulate the immune system. It is also used to treat chronic constipation of the elderly and debilitated as well as menstrual disorders. Research has demonstrated that Danggui and its active ingredients, as anti-arthrosclerotic, anti-hypertensive, antioxidant anti-inflammatory agents which would limit platelet aggregation, are effective in reducing the size of cerebral infarction and improving neurological deficit scores.

2011-01-01

15

Organic petrography and geochemistry of three samples from Dong Ho, Vietnam.  

National Technical Information Service (NTIS)

This report presents the results of an organic petrographic and geochemical study of three outcrop samples from Dong Ho, northern Vietnam. The objective of the study is to assess the composition and source rock potential of the organic matter. The analyti...

H. Ingermann Petersen A. Bojesen-Koefoed L. H. Nielsen H. P. Nytoft

1998-01-01

16

Thermochemistry and kinetics of angelica and cognate lactones.  

PubMed

The enthalpies of formation, bond dissociation energies, ionization potentials, and kinetics of reaction with hydrogen atoms and methyl radicals have been systematically calculated for angelica lactone and a number of related furanones. The objective was to provide comprehensive thermodynamic and kinetic data of compounds that are projected to play a role as intermediates in the production of platform chemicals and biofuels. PMID:24828300

Wurmel, Judith; Simmie, John M

2014-06-12

17

Herb-drug interactions  

Microsoft Academic Search

Concurrent use of herbs may mimic, magnify, or oppose the effect of drugs. Plausible cases of herb-drug interactions include: bleeding when warfarin is combined with ginkgo (Ginkgo biloba), garlic (Allium sativum), dong quai (Angelica sinensis), or danshen (Salvia miltiorrhiza); mild serotonin syndrome in patients who mix St John's wort (Hypericum perforatum) with serotonin-reuptake inhibitors; decreased bioavailability of digoxin, theophylline, cyclosporin,

Adriane Fugh-Berman

18

Genetic Polymorphism of Mitochondrial DNA in Dong, Gelao, Tujia, and Yi Ethnic Populations from Guizhou, China  

Microsoft Academic Search

To reveal the genetic structures and relationships of the four ethnic populations from the maternal inheritance and explore the origins and migrations of nationalities, the genetic polymorphism of mtDNA in Dong, Gelao, Tujia, and Yi populations from Guizhou was studied by direct sequencing of hypervariable segment I(HVS I) and PCR-RFLP of coding region. Thirty-seven (sub-) haplogroups were identified in the

Binbin Li; Fuguang Zhong; Hongsheng Yi; Xianran Wang; Liangfang Li; Lilan Wang; Xiaolan Qi; Lifu Wu

2007-01-01

19

A new coumarin from the stem of Angelica dahurica.  

PubMed

One new and three known coumarins were isolated from the CHCl3 soluble fraction of Angelica dahurica stem. On the basis of spectral data, the structures of the isolated compounds were determined to be scopoletin, angelol I, angelol H and 6-[(1S), 2(R)-2, 3-dihydroxy-1-methoxy-3-methylbutyl]-7-methoxycoumarin; the latter being isolated for the first time from a plant source. PMID:11885692

Kwon, Yong Soo; Shin, Soo Jung; Kim, Myong Jo; Kim, Chang Min

2002-02-01

20

Estrogenic activity of furanocoumarins isolated from Angelica dahurica.  

PubMed

In our efforts to discover novel phytoestrogens to treat menopausal symptoms, eleven furanocoumarins were isolated from Angelica dahurica and tested for their estrogenic activity on the Ishikawa cell line. Among the compounds tested, 9-hydroxy-4-methoxypsoralen and alloisoimperatorin showed strong abilities to induce alkaline phosphatase (AP) with EC50 values of 1.1 and 0.8 microg/mL, respectively, whereas the other nine furanocoumarins were weakly or only slightly active. PMID:17024846

Piao, Xiang Lan; Yoo, Hye Hyun; Kim, Hyun Young; Kang, Tak Lim; Hwang, Gwi Seo; Park, Jeong Hill

2006-09-01

21

Agents protecting against sepsis from the roots of Angelica dahurica.  

PubMed

In the course of isolating agents preventing sepsis from the EtOAc extract of the roots of Angelica dahurica, four known furanocoumarins, isoimperatorin (1), oxypeucedanin (2), (+/-)-byakangelicin (3), and (+)-oxypeucedanin hydrate (4), were isolated as active compounds based on the in vivo assay model of sepsis induced by lipopolysaccharide (LPS) and D-galactosamine (D-GalN). Among them, 3 showed the highest survival rate (100% with a dose of 30 mg/kg versus 20% for the control experiment) and decreased the plasma levels of tumor necrosis factor-alpha and alanine aminotransferase in mice adminstered LPS/D-GalN. PMID:15684506

Song, Dong-Keun; Kim, Ji-Yeon; Li, Gao; Lee, Kyeung-Seon; Seo, Chang-Seob; Yan, Ji-Jing; Jung, Jun-Sub; Kim, Hyo-Jin; Chang, Hyun-Wook; Son, Jong-Keun

2005-02-01

22

Acetylcholinesterase inhibitors from the roots of Angelica dahurica.  

PubMed

In the course of finding Korean natural products for acetylcholinesterase (AChE) inhibitory activity, we found that a methanolic extract of the roots of Angelica dahurica showed significant inhibitory effects on AChE. Bioassay-guided fractionation of the methanolic extract resulted in the isolation of three furanocoumarins, isoimperatorin (1), imperatorin (2) and oxypeucedanin (3), as active principles. These compounds inhibited AChE activity in a dose-dependent manner, and the IC50 values of compounds 1-3 were 74.6, 63.7 and 89.1 microM, respectively. PMID:12510838

Kim, Dae Keun; Lim, Jong Pil; Yang, Jae Heon; Eom, Dong Ok; Eun, Jae Soon; Leem, Kang Hyun

2002-12-01

23

Angelica sinensis suppresses human lung adenocarcinoma A549 cell metastasis by regulating MMPs/TIMPs and TGF-?1.  

PubMed

In this study we investigated the potential effects of Angelica sinensis on the growth and metastasis in human lung adenocarcinoma A549 cells. In vitro the Cck-8 assays showed that Angelica sinensis had weak antiproliferative effect on A549 cells only at high concentration. The cell adhesion assay showed that Angelica sinensis decreased the adhesive ability of A549 cells in a dose- and time-dependent manner. Transwell invasion and migration assay showed that Angelica sinensis reduced the invasive and migratory abilities of A549 cells in a dose-dependent manner. In vivo the animal experiments showed that Angelica sinensis suppressed lung metastasis of nude mice at high concentration. Then, we attempted to clarify the mechanisms of anti-metastatic activities of Angelica sinensis. The results showed Angelica sinensis inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), it involved the down-regulation of the expressions of MMP-2 and MMP-9 at both the protein and mRNA levels, which may be associated with Angelica sinensis suppressing the expression of TGF-?1. It also involved the increase of the tissue inhibitors of metalloproteinases TIMP-2, but TIMP-1 decreased upon incubation of A549 cells with Angelica sinensis. The results suggest that Angelica sinensis might exert anti-growth and anti-metastasis activity against lung cancer cells through the decrease of MMP-2, MMP-9, TGF-?1 and TIMP-1 and increase of TIMP-2. PMID:22076386

Gao, Min; Zhang, Jun-Hong; Zhou, Fu-Xiang; Xie, Cong-Hua; Han, Guang; Fang, Sheng-Quan; Zhou, Yun-Feng

2012-02-01

24

Preparation of blood-deficient model and research of angelica polysaccharide on enriching blood in chickens.  

PubMed

In this study cyclophosphamide was used to prepare the blood-deficient model. The red blood cell count and hemoglobin content were measured. The experimental chickens presented the symptoms of blood-deficient syndrome, dullness, shrinkinginto oneself, broken winded, loose feather, waxy eyelid, and pale tongue. At the same time, red blood cell count and hemoglobin content decreased significantly. Angelica polysaccharide as the effective component of Angelica Sinensis could significantly increase the red blood cell count and the hemoglobin content of blood-deficient chickens. The results indicated that cyclophosphamide could significantly reduce the red blood count and hemoglobin content, and make the ideal blood-deficient model successfully. Angelica polysaccharide had the function of enriching blood in different ways. On the one hand Angelica polysaccharide enriched he blood directly, increased the number of RBC and hemoglobin; on the other hand it regulated the hematopoietic factors, enriched the blood indirectly. PMID:22675393

Hou, Haifeng; Bao, Yongzhan; Li, Qian; Shi, Wanyu

2012-01-01

25

[Analysis of the 4th generation outer space bred angelica dahurica by FTIR spectroscopy].  

PubMed

The major components of the 4th generation outer space bred angelica and the ground group were determined and analyzed by Fourier transform infrared spectroscopy (FTIR) and second derivative spectrum, considering the large mutation of the plants with space mutagenesis. The results show that the content of the coumarin (1741 cm(-1)), which is the main active components of the space angelica dahurica increased, and the content of the protein (1 459, 1 419 cm(-1)) and the fat (930 cm(-1)) increased slightly, whereas the content of the starch and the dietary fiber reduced drastically. There are obvious differences between the peak values of the second derivative spectra of the plants, revealing that the outer space angelica dahurica contained amine component at 1 279 cm(-1). Space mutation breeding is favor of breeding angelica with better idiosyncrasy. PMID:22582627

Zhu, Yan-ying; Wu, Peng-le; Liu, Mei-yi; Wang, Zhi-zhou; Guo, Xi-hua; Guan, Ying

2012-03-01

26

Evaluation of Antiseizure Activity of Essential Oil from Roots of Angelica archangelica Linn. in Mice.  

PubMed

In the present study, the effect of essential oil of the root of Angelica archangelica Linn. was evaluated against electrically and chemically induced seizures. The seizures were induced in mice by maximal electroshock and pentylenetetrazol. The effect of essential oil of the root of Angelica archangelica on seizures was compared with standard anticonvulsant agents, phenytoin and diazepam. The essential oil of the root of Angelica archangelica suppressed duration of tonic convulsions and showed recovery in maximal electroshock induced seizures while it delayed time of onset of clonic convulsions and showed mortality protection in pentylenetetrazol induced seizures. The essential oil of the root of Angelica archangelica also produced motor impairment at the antiseizure doses. The study indicated that the essential oil exhibited antiseizure effect. The antiseizure effect may be attributed to the presence of terpenes in the essential oil. PMID:21188050

Pathak, Shalini; Wanjari, M M; Jain, S K; Tripathi, M

2010-05-01

27

Evaluation of Antiseizure Activity of Essential Oil from Roots of Angelica archangelica Linn. in Mice  

PubMed Central

In the present study, the effect of essential oil of the root of Angelica archangelica Linn. was evaluated against electrically and chemically induced seizures. The seizures were induced in mice by maximal electroshock and pentylenetetrazol. The effect of essential oil of the root of Angelica archangelica on seizures was compared with standard anticonvulsant agents, phenytoin and diazepam. The essential oil of the root of Angelica archangelica suppressed duration of tonic convulsions and showed recovery in maximal electroshock induced seizures while it delayed time of onset of clonic convulsions and showed mortality protection in pentylenetetrazol induced seizures. The essential oil of the root of Angelica archangelica also produced motor impairment at the antiseizure doses. The study indicated that the essential oil exhibited antiseizure effect. The antiseizure effect may be attributed to the presence of terpenes in the essential oil.

Pathak, Shalini; Wanjari, M. M.; Jain, S. K.; Tripathi, M.

2010-01-01

28

Phytochemical and pharmacological studies on Radix Angelica sinensis.  

PubMed

The roots of Angelica sinensis (RAS), are a Chinese herbal medicine traditionally used in prescriptions for replenishing blood, treating abnormal menstruation, and other women's diseases. It has also been widely marketed as health food for women's care in Asia, and as a dietary supplement in Europe and America. RAS is well-known for its hematopoietic, antioxidant, and immunoregulatory activities. RAS also possesses anti-cancer, memory, radioprotective, and neuroprotective effects. Phytochemical investigations on this plant led to organic acids, phthalides, polysaccharides, and other metabolites. Based on recent animal studies and clinical trials, RAS has been used in the treatment of gynecologic diseases, cardio-cerebrovascular disease, nervous system diseases, and nephrotic syndrome. In this review, the recent phytochemical and pharmacological studies, drug-drug interactions, clinical applications, and toxicity of RAS are summarized. PMID:24345498

Chen, Xiao-Peng; Li, Wei; Xiao, Xue-Feng; Zhang, Lan-Lan; Liu, Chang-Xiao

2013-11-01

29

Cytotoxic coumarins from the root of Angelica dahurica.  

PubMed

Ten coumarins were isolated from the root of Angelica dahurica by repeated silica gel column chromatography. Their chemical structures were elucidated on the basic of physicochemical and spectroscopic data. Among them, oxypeucedanin hydrate acetonide (7) was isolated for the first time from this plant. Cytotoxicity of coumarins isolated were determined in vitro against L1210, HL-60, K562, and B16F10 tumor cell lines by MTT method. Pangelin (5) and oxypeucedanin hydrate acetonide (7) showed a potent cytotoxic activity with the IC50 values of 8.6 to 14.6 microg/mL against four kinds of tumor cell lines. Other compounds showed the moderate cytotoxic activity or no activity against the tumor cell lines. PMID:15646793

Thanh, Pham Ngoc; Jin, WenYi; Song, GyuYong; Bae, KiHwan; Kang, Sam Sik

2004-12-01

30

Dendritic cell activation by polysaccharide isolated from Angelica dahurica.  

PubMed

Angelica dahurica is used in functional foods for the prevention and treatment of various diseases, such as inflammation and cancer. In the present study, we examined the effect of A. dahurica polysaccharide (ADP) on dendritic cell (DC) maturation. ADP increased the expressions of CD86 and MHC-II molecules, the production of IL-12, IL-1?, and TNF-?, and allogeneic T cell activation ability of DCs, and reduced DC endocytosis. As a mechanism of action, the knockdown of TLR4 with small interfering RNA decreased the ADP-induced production of nitric oxide and IL-12 by DCs, suggesting the membrane receptor candidate of ADP. After binding to TLR4, ADP increased the phosphorylation of ERK, JNK, and p38 MAPKs, and the nuclear translocation of NF-?B p50/p65. These results indicate that ADP activates DCs through TLR4 and downstream signalings. PMID:23246459

Kim, Hyung Sook; Shin, Bo Ram; Lee, Hong Kyung; Park, Yun Soo; Liu, Qing; Kim, Sung Yeon; Lee, Mi Kyeong; Hong, Jin Tae; Kim, Youngsoo; Han, Sang-Bae

2013-05-01

31

The anti-staphylococcal activity of Angelica dahurica (Bai Zhi).  

PubMed

Bioassay-guided fractionation of a hexane extract prepared from the roots of the Chinese drug Angelica dahurica (Bai Zhi) led to the isolation of the polyacetylenic natural product falcarindiol (1). The absolute stereochemistry of this compound was confirmed by careful 1H NMR analysis of its (R)- and (S)-Mosher ester derivatives as the 3(R), 8(S) isomer. Activity was tracked using a Mycobacterium fortuitum screening assay and the purified product was evaluated against multidrug-resistant and methicillin-resistant strains of Staphylococcus aureus (MRSA). The minimum inhibitory concentrations (MIC) of this metabolite ranged from 8 to 32 microg/ml highlighting the potential of the acetylene natural product class as antibiotic-lead compounds. These MIC values compare favourably with some of the newest agents in development for the treatment of MRSA infection and indicate that further evaluation of the antibiotic activity of acetylenes is warranted. PMID:14751304

Lechner, Doris; Stavri, Michael; Oluwatuyi, Moyosoluwa; Pereda-Miranda, Rogelio; Gibbons, Simon

2004-02-01

32

Molecular systematics of Angelica and allied genera (Apiaceae) from the Hengduan Mountains of China based on nrDNA ITS sequences: phylogenetic affinities and biogeographic implications  

Microsoft Academic Search

Maximum parsimony, maximum likelihood, and Bayesian analyses of nuclear ribosomal DNA internal transcribed spacer sequences\\u000a were used to infer the phylogenetic affinities and historical biogeography of Angelica and its allies (Apiaceae tribe Selineae), with emphasis on those species of Angelica and Peucedanum endemic to the Hengduan Mountains of south-central China. Results of these analyses corroborate a monophyletic Angelica (Angelica sensu

Tu Feng; Stephen R. Downie; Yan Yu; Xuemei Zhang; Weiwei Chen; Xingjin He; Shuang Liu

2009-01-01

33

Importance of wine-treated Angelica Sinensis Radix in Si Wu Tang, a traditional herbal formula for treating women's ailments.  

PubMed

Si Wu Tang (Four Agents Decoction), a traditional Chinese decoction composed of Angelica Sinensis Radix, Chuanxiong Rhizoma, Paeoniae Radix Alba, and Rehmanniae Radix Praeparata in a ratio of 1 : 1 : 1 : 1, has been used to treat women's diseases for more than a thousand years. According to the original description of Si Wu Tang, Angelica Sinensis Radix should be treated with wine. However, the importance of this wine-treated Angelica Sinensis Radix in Si Wu Tang's function has not been identified. In this article, the chemical and biological properties of two decoctions processed in different ways (Si Wu Tang with crude Angelica Sinensis Radix and Si Wu Tang with wine-treated Angelica Sinensis Radix) were compared for examination. The herbal decoction Si Wu Tang prepared from wine-treated Angelica Sinensis Radix contained much different amounts of its active compounds. Compared with Si Wu Tang using crude Angelica Sinensis Radix, Si Wu Tang prepared from wine-treated Angelica Sinensis Radix had better biological responses. Therefore, these findings accentuate the functional importance of herbs treated with wine in the Chinese decoction. PMID:23457023

Zhan, Janis Y X; Zheng, Ken Y Z; Zhu, Kevin Y; Zhang, Wendy L; Bi, Cathy W C; Chen, J P; Du, Crystal Y Q; Dong, Tina T X; Lau, David T W; Tsim, Karl W K

2013-05-01

34

Persistent Organic Pollutants in Sediments from Sai Gon–Dong Nai River Basin, Vietnam: Levels and Temporal Trends  

Microsoft Academic Search

Surficial sediment samples were collected from Hochiminh City canals, the Sai Gon–Dong Nai River, and its estuary, one of\\u000a the most predominant industrial areas in Hochiminh City, southern Vietnam, for determination of selected persistent organic\\u000a pollutants (POPs). Contamination pattern was as follows: PCBs ? DDTs > HCB > CHLs > HCHs. Concentrations of PCBs and DDTs\\u000a ranged from 0.50–150 ng\\/g

Nguyen Hung Minh; Tu Binh Minh; Hisato Iwata; Natsuko Kajiwara; Tatsuya Kunisue; Shin Takahashi; Pham Hung Viet; Bui Cach Tuyen; Shinsuke Tanabe

2007-01-01

35

Effect of Bamboo Vinegar on the Growth and Flowering of Potted Paeonia lactiflora 'Dong Fang Hong' in Greenhouse  

Microsoft Academic Search

The aim of this paper is to study the effect of bamboo vinegar,a natural and environmental-friendly liquid extracted from bamboo, on growth and flowering of potted herbaceous peony 'Dong Fang Hong' in greenhouse. The 300,500, 800 and 1000 times diluted solutions of bamboo vinegar were sprayed on the leaves and stems of the peonies respectively, 1 time on the leaf

Jing Han; Wei Wang; Jin-Gen Peng; Yan Liu

2011-01-01

36

Metabolic fingerprinting of Angelica sinensis during growth using UPLC-TOFMS and chemometrics data analysis  

PubMed Central

Background The radix of Angelica sinensis is widely used as a medicinal herbal and metabolomics research of this plant during growth is necessary. Results Principal component analysis of the UPLC-QTOFMS data showed that these 27 samples could be separated into 4 different groups. The chemical markers accounting for these separations were identified from the PCA loadings plot. These markers were further verified by accurate mass tandem mass and retention times of available reference standards. The study has shown that accumulation of secondary metabolites of Angelica sinensis is closely related to the growth periods. Conclusions The UPLC-QTOFMS based metabolomics approach has great potential for analysis of the alterations of secondary metabolites of Angelica sinensis during growth.

2013-01-01

37

Evaluation of acute skin irritation and phototoxicity by aqueous and ethanol fractions of Angelica keiskei  

PubMed Central

In this study, to assess whether aqueous and ethanol fractions of Angelica keiskei induce acute skin irritation and phototoxicity, acute skin irritancy and phototoxicity tests were performed. The skin of rabbits or guinea pigs was treated with these fractions (100 mg/dose) and whether the animals sustained significant skin damage was determined. The data demonstrated that the aqueous and ethanol fractions of Angelica keiskei did not induce acute toxicity in the skin of the animals, as assessed by anatomical and pathological observations. The results from the present study suggest that these aqueous and ethanol fractions of Angelica keiskei have promising potential uses as cosmetic ingredients that do not induce significant levels of skin irritation or phototoxicity.

LEE, SANG-HAN

2013-01-01

38

New cosmetic agents for skin whitening from Angelica dahurica.  

PubMed

To develop a new whitening agent for cosmetics from natural products, Angelica dahurica was selected for its inhibitory effect on melanogenesis in B16 melanoma cells. From the mechanism study, it was clarified that the ethanolic extracts of this plant showed the suppression of tyrosinase synthesis but no inhibition of tyrosinase activity. In order to find the active constituents from this plant, the ethanol extracts were chromatographed repeatedly with silica gel. Two coumarin compounds were isolated from A. dahurica. Their structures were identified by physicochemical and spectral data such as UV, IR, NMR, and MS. It was shown that the active substance was isoimperatorin (10-[(3-methyl-2-butenyl)oxy]-7H-furo[3,2-g][1] benzopyran-7-one) and imperatorin (9-[(3-methyl-2-butenyl)oxy]-7H-furo[3,2-g][1] benzopyran-7-one). They significantly inhibited tyrosinase synthesis in B16 melanoma cells. To elucidate the action mechanism of the active compounds of A. dahurica, we investigated the changes in the mRNA level of tyrosinase using the RT-PCR technique. As a result, the mRNA level of tyrosinase was markedly reduced by active compounds of A. dahurica. From these results, we suggest that these extracts might be useful as a new whitening agent in cosmetics, but the in vitro findings must be verified in in vivo skin-lightening studies. PMID:16676120

Cho, Y H; Kim, J H; Park, S M; Lee, B C; Pyo, H B; Park, H D

2006-01-01

39

[Study of the antimutagenic properties of Angelica archangelica by the micronucleus test].  

PubMed

The antimutagenic activity Angelica archangelica L. water and alcohol extracts thio-tepa against mutagenicity was investigated by the micronucleus test in mouse bone marrow and peripheral blood cells. The reduction of thio-tepa mutagenic activity was more prominent when the extracts were injected 2-hours before thio-tepa treatment as it could be seen at the simultaneous treatment. The observed reduction of micronucleus frequencies was up to 77%. No genotoxic effects of Angelica extracts had been seen at the concentrations 50-100 mg/kg. PMID:8049396

Salikhova, R A; Dulatova, Sh N; Poroshenko, G G

1993-04-01

40

2D NMR spectroscopic analyses of archangelicin from the seeds of Angelica archangelica.  

PubMed

A total of six coumarins, bergapten (1), xanthotoxin (2), imperatorin (3), isoimperatorin (4), phellopterin (5) and archangelicin (6), have been isolated from an n-hexane extract of the seeds of Angelica archangelica. The results of comprehensive 2D NMR analyses of archangelicin are discussed. PMID:15634612

Muller, Melanie; Byres, Maureenx; Jaspars, Marcel; Kumarasamy, Yashodharan; Middleton, Moira; Nahar, Lutfun; Shoeb, Mohammad; Sarker, Satyajit D

2004-12-01

41

Relationship between HPLC fingerprints and in vivo pharmacological effects of a traditional Chinese medicine: Radix Angelicae Dahuricae.  

PubMed

We report the fingerprint development of a traditional Chinese medicine Radix Angelicae Dahuricae root and the correlation of the fingerprint peaks with its in vivo pharmacological effects. The high-performance liquid chromatography (HPLC) methods with the computer aided similarity evaluation were validated and used in serial pharmacological studies in mice. The major constituents of R. Angelicae Dahuricae were successfully separated by the HPLC methods, and the effects of sedation and analgesia were mainly related to the chromatographic peaks of group II. The anti-inflammatory, anti-heat stroke and anti-endotoxemic effects were mainly related to the peaks in group III. These results indicated a correlation between the HPLC fingerprints in groups and the pharmacological effects of R. Angelicae Dahuricae. This simple and accurate method can be used for the identification of the active components of R. Angelicae Dahuricae and for the quality control of its pharmaceutical preparations. PMID:21240762

Nie, Hong; Zhang, Hui; Zhang, Xiao-qi; Luo, Yong; Meng, Lan-zhen; Yin, Zhen; Zhang, Jianyu; Li, Kongyan; Xiong, Ai-hua; Zhong, Ling; Huang, Heqing; Ye, Wen-cai

2011-01-01

42

Ultrafine Angelica gigas Powder Normalizes Ovarian Hormone Levels and Has Antiosteoporosis Properties in Ovariectomized Rats: Particle Size Effect  

PubMed Central

Abstract The root of Angelica gigas (Korean angelica) is traditionally used to treat women's ailments that are caused by an impairment of menstrual blood flow and cycle irregularities. This study evaluated the effect particle size of Korean angelica powder on its efficacy for treating estrogen-related symptoms of menopause. Initially, Korean angelica roots were pulverized into ultrafine powder, and orally administered to the rats at a concentration of 500?mg/kg body weight for 8 weeks. The effects of Korean angelica powder particle size on extraction yield, contents of bioactive compounds (decursin and decursinol angelate), levels of serum ovarian hormones (estradiol and progesterone), reproductive hormones (luteinizing hormone and follicle-stimulating hormone), and experimental osteoporosis parameters (mineral density, strength, and histological features) were determined. A significant increase (fivefold) in the contents of decursin and decursinol angelate in the extract of the ultrafine Korean angelica powder was observed compared to coarse Korean angelica powder. Rats were divided into sham-operated or ovariectomized (OVX) groups that were fed coarse (CRS) or ultrafine (UF) ground Korean angelica root. The serum levels of estradiol in the OVX_UF group were 19.2% and 54.1% higher than that of OVX_CRS group. Serum bone-alkaline phosphatase/total-alkaline phosphatase index in the OVX_UF group was half that of the OVX_CRS group. In addition, less trabecular bone loss and thick cortical areas were observed in rats administered ultrafine powder. Therefore, ultrafine grinding may enhance the bioactivity of herbal medicines and be especially useful when their extracted forms lose bioactivity during processing, storage, and oral intake.

Choi, Kyeong-Ok; Lee, Inae; Paik, Sae-Yeol-Rim; Kim, Dong Eun; Lim, Jung Dae; Kang, Wie-Soo; Ko, Sanghoon

2012-01-01

43

Chemical and biological assessment of angelica roots from different cultivated regions in a chinese herbal decoction danggui buxue tang.  

PubMed

Roots of Angelica sinensis (Danggui) have been used in promoting blood circulation as herbal medicine for over 2000 years in China. Another species of Angelica roots called A. gigas is being used in Korea. To reveal the efficiency of different Angelica roots, the chemical and biological properties of Angelica roots from different cultivated regions were compared. Roots of A. sinensis contained higher levels of ferulic acid, Z-ligustilide, and senkyunolide A, while high amounts of butylphthalide and Z-butylenephthalide were found in A. gigas roots. The extracts deriving from A. gigas roots showed better effects in osteogenic and estrogenic properties than that of A. sinensis from China. However, this difference was markedly reduced when the Angelica roots were being prepared in a Chinese herbal decoction together with Astragali Radix as Danggui Buxue Tang. In contrast, the herbal decoction prepared from A. sinensis roots showed better responses in cell cultures. In addition, the extracts of A. gigas roots showed strong cell toxicity both as single herb and as Danggui Buxue Tang. This result revealed the distinct properties of Angelica roots from China and Korea suggesting the specific usage of herb in preparing a unique herbal decoction. PMID:23476692

Zhang, Wendy L; Zheng, Ken Y Z; Zhu, Kevin Y; Zhan, Janis Y X; Bi, Cathy W C; Chen, J P; Dong, Tina T X; Choi, Roy C Y; Lau, David T W; Tsim, Karl W K

2013-01-01

44

Chemical and Biological Assessment of Angelica Roots from Different Cultivated Regions in a Chinese Herbal Decoction Danggui Buxue Tang  

PubMed Central

Roots of Angelica sinensis (Danggui) have been used in promoting blood circulation as herbal medicine for over 2000 years in China. Another species of Angelica roots called A. gigas is being used in Korea. To reveal the efficiency of different Angelica roots, the chemical and biological properties of Angelica roots from different cultivated regions were compared. Roots of A. sinensis contained higher levels of ferulic acid, Z-ligustilide, and senkyunolide A, while high amounts of butylphthalide and Z-butylenephthalide were found in A. gigas roots. The extracts deriving from A. gigas roots showed better effects in osteogenic and estrogenic properties than that of A. sinensis from China. However, this difference was markedly reduced when the Angelica roots were being prepared in a Chinese herbal decoction together with Astragali Radix as Danggui Buxue Tang. In contrast, the herbal decoction prepared from A. sinensis roots showed better responses in cell cultures. In addition, the extracts of A. gigas roots showed strong cell toxicity both as single herb and as Danggui Buxue Tang. This result revealed the distinct properties of Angelica roots from China and Korea suggesting the specific usage of herb in preparing a unique herbal decoction.

Zhang, Wendy L.; Zheng, Ken Y. Z.; Zhu, Kevin Y.; Zhan, Janis Y. X.; Bi, Cathy W. C.; Chen, J. P.; Dong, Tina T. X.; Choi, Roy C. Y.; Lau, David T. W.; Tsim, Karl W. K.

2013-01-01

45

Characteristic of alkylated chalcones from Angelica keiskei on influenza virus neuraminidase inhibition  

Microsoft Academic Search

As part of our ongoing effort to develop influenza virus neuraminidase (NA) inhibitors from various medicinal plants, we utilized bioassay-guided fractionation to isolated six alkylated chalcones (1–6) from Angelica keiskei. Xanthokeistal A (1) emerged as new compound containing the rare alkyl substitution, 6,6-dimethoxy-3-methylhex-2-enyl. When we tested the ability of these individual alkyl substituted chalcones to inhibit influenza virus NA hydrolysis,

Ji-Young Park; Hyung Jae Jeong; Young Min Kim; Su-Jin Park; Mun-Chual Rho; Ki Hun Park; Young Bae Ryu; Woo Song Lee

2011-01-01

46

[The enhancing effect of Angelica dahurica extracts on absorption of baicalin--the active composition of Scutellaria].  

PubMed

To explore the mechanism of the absorption enhancement of Angelica dahurica extract (Ade), the absorption mechanism of baicalin in the Scutcllaria water extraction as well as the effect of Angelica dahurica extract on absorption of baicalin were investigated. In order to determine the main absorption site, everted intestinal sac model was used to study the effect of Angelica dahurica extract on the absorption of baicalin at duodenum, jejunum, ileum and colon. In situ single pass intestinal perfusion model was performed to study the absorption of various concentrations of baicalin and the effect of Angelica dahurica extract on the absorption of baicalin at the main absorption site. To authenticate the consequence of perfusion by getting the blood from the hepatic portal vein and determine the concentration of the baicalin in the blood. The result showed that baicalin could be absorbed at all of the four intestinal segments with increasing absorption amount per unit as follows: ileum > colon > jejunum > duodenum. The absorption ofbaicalin in the duodenum significantly increased with Angelica dahurica extract, thus, duodenum was chosen to be the studying site. Apparent permeability values (Papp) and absorption rate constant (Ka) of baicalin in the duodenum increased gradually with higher concentrations. When the concentration of baicalin rises to a certain degree, the absorption increase had a saturable process, the absorption of baicalin may be an active transportation. Baicalin may be not a substrate of P-gp as verapamil which had not significantly affected the Papp and Ka of baicalin. The absorption of baicalin in the duodenum significantly increased (P < 0.01) in the two models with Angelica dahurica extract and the concentration of baicalin in the blood from the hepatic portal vein showed that the Angelica dahurica extract can increase the absorption of baicalin. PMID:21542296

Zhu, Jing-yun; Liang, Xin-li; Wang, Guang-fa; Zhao, Guo-wei; Liao, Zheng-gen; Cao, Yun-chao; Chen, Xu-long; Yang, Ming

2011-02-01

47

Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs.  

PubMed

A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures. PMID:15909545

Lu, Guang-Hua; Chan, Kelvin; Liang, Yi-Zeng; Leung, Kelvin; Chan, Chi-Leung; Jiang, Zhi-Hong; Zhao, Zhong-Zhen

2005-05-01

48

Supercritical fluid extraction for the separation of organochlorine pesticides residue in Angelica sinensis.  

PubMed

A method involving the simultaneous extraction and separation of 12 organochlorine pesticides (OCPs) from Angelicae sinensis was developed using supercritical fluid extraction (SFE). The pesticides in the study were alpha-, beta-, gamma- and delta-benzene hexachloride, PCNB (pentachloro- nitrobenzene), PCA (pentachloroaniline), HEPT (heptachlor), MPCPS (methyl-pentachlorophenyl sulfide), pp'-DDE [1,1-dichloro-2,2-bis (p-chlorophenyl) ethylene], op'-DDT [1,1,1,-trichloro-2-(o-chlorophenyl)-2-(p-chlorophenyl) ethane], pp'-DDD [1,1-dichloro-2,2-bis(p-chlorophenyl) ethane], and pp'-DDT [1,1,1,-trichloro-2,2-bis (p-chlorophenyl)ethane]. The extraction conditions were optimized as follows: pure CO(2), extraction pressure 15 MPa, extraction temperature 60 degrees C, extraction time 20 min, and flow-rate 1.5 mL/min. A GC method with electron capture detection was employed to determine the OCPs in Angelicae sinensis. An HPLC method was developed for the quantitative determination of active constituents. The SFE provided high decontamination rate of OCPs and low loss of active constituents in Angelicae sinensis. PMID:12378554

Zhao, Chunjie; Hao, Guiming; Li, Huanxin; Chen, Yingjie

2002-10-01

49

[Immunohistochemical analysis and immuno-gold localization of ECBP21 in Angelica dahurica].  

PubMed

ECBP21 is a calmodulin binding protein (CaMBP) purified from extracellular extracts of suspension-cultured cells of Angelica dahurica, and it is the first reported extracellular CaMBP in plant kingdom. We have recently cloned the full-length cDNA for ECBP21. In this work, using recombinant ECBP21 we prepared rabbit antiserum with high specificity and high titer against ECBP21, and investigated the organ-specific distribution of ECBP21 in Angelica dahurica. ECBP21 was found in all organs examined, particularly abundant in the leaves flowers, and raches, and less in the roots. It was also found in all cells examined, and particularly enriched in the cell wall. These data support the notion that ECBP21 is specifically localized extracellularly, and imply that it may be involved in plant growth and development. In addition, using immunogold transmission electron microscopy method, we studied the subcellular localization of ECBP21 in rachis cells of Angelica dahurica. The results indicated that the ECBP21 was mainly localized in cell wall; this provided a direct evidence of the extracellular existence of ECBP21. PMID:15133894

Ding, Cun Bao; Mao, Guo Hong; Sun, Da Ye

2004-02-01

50

In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis , Fructus bruceae , Caulis spatholobi , Semen aesculi , and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish ( Carassius auratus )  

Microsoft Academic Search

In search of a natural antiparasitic, in vivo anthelmintic activity of petroleum ether, chloroform, ethyl acetate, methanol,\\u000a and aqueous extracts of Angelica pubescens roots (Radix angelicae pubescentis), Brucea javanica fruits (Fructus bruceae), Spatholobus suberectus stems (Caulis spatholobi), Aesculus chinensis Bge. seeds (Semen aesculi), and Pharbitis purpurea (L.) Voigt seeds (Semen pharbitidis) were tested against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius

You-Tao Liu; Fan Wang; Gao-Xue Wang; Jing Han; Yong Wang; Yong-Hua Wang

2010-01-01

51

A Systematic Review of the Mysterious Caterpillar Fungus Ophiocordyceps sinensis in Dong-ChongXiaCao ( D?ng Chóng Xià C?o) and Related Bioactive Ingredients.  

PubMed

The caterpillar fungus Ophiocordyceps sinensis (syn.Cordyceps sinensis), which was originally used in traditional Tibetan and Chinese medicine, is called either "yartsa gunbu" or "DongChongXiaCao ( D?ng Chóng Xià C?o)" ("winter worm-summer grass"), respectively. The extremely high price of DongChongXiaCao, approximately USD $20,000 to 40,000 per kg, has led to it being regarded as "soft gold" in China. The multi-fungi hypothesis has been proposed for DongChongXiaCao; however, Hirsutella sinensis is the anamorph of O. sinensis. In Chinese, the meaning of "DongChongXiaCao" is different for O. sinensis, Cordyceps spp., and Cordyceps sp. Over 30 bioactivities, such as immunomodulatory, antitumor, anti-inflammatory, and antioxidant activities, have been reported for wild DongChongXiaCao and for the mycelia and culture supernatants of O. sinensis. These bioactivities derive from over 20 bioactive ingredients, mainly extracellular polysaccharides, intracellular polysaccharides, cordycepin, adenosine, mannitol, and sterols. Other bioactive components have been found as well, including two peptides (cordymin and myriocin), melanin, lovastatin, ?-aminobutyric acid, and cordysinins. Recently, the bioactivities of O. sinensis were described, and they include antiarteriosclerosis, antidepression, and antiosteoporosis activities, photoprotection, prevention and treatment of bowel injury, promotion of endurance capacity, and learning-memory improvement. H. sinensis has the ability to accelerate leukocyte recovery, stimulate lymphocyte proliferation, antidiabetes, and improve kidney injury. Starting January 1(st), 2013, regulation will dictate that one fungus can only have one name, which will end the system of using separate names for anamorphs. The anamorph name "H. sinensis" has changed by the International Code of Nomenclature for algae, fungi, and plants to O. sinensis. PMID:24716152

Lo, Hui-Chen; Hsieh, Chienyan; Lin, Fang-Yi; Hsu, Tai-Hao

2013-01-01

52

Bioactivity-guided fractionation and GC/MS fingerprinting of Angelica sinensis and Angelica archangelica root components for antifungal and mosquito deterrent activity.  

PubMed

Bioassay-guided fractionation of the chloroform extract from the roots of Angelica sinensis led to isolation and characterization of (Z)-ligustilide using direct-bioautography with Colletotrichum species. The structure of (Z)-ligustilide was confirmed by (1)H and (13)C NMR spectroscopy and GC/MS. (Z)-Ligustilide deterred the biting of two mosquito species more effectively than DEET. Three different A. sinensis accessions and one Angelica archangelica root oil were evauated by GC and GC/MS, and the dominant component in A. sinensis was 61-69% (Z)-ligustilide. Two other prominent compounds in A. sinensis oils were 5.7-9.8% (E)-3-butylidene phthalide and 1.5-2.3% (Z)-3-butylidene phthalide. The main constituents that comprised A. archangelica oil were monoterpene hydrocarbons such as 24.5% alpha-pinene, 13.8% delta-3-carene, 10.1% beta-phellandrene, 8.8% p-cymene, 8.4% limonene, and 6.3% sabinene. Phthalides and monoterpene hydrocarbons were determined to be good systematic markers or chemical fingerprints for A. sinensis and A. archangelica root oils. Chemical fingerprinting by GC/MS of A. sinensis also confirmed the misidentification of one A. archangelica sample sold in the Chinese market. PMID:19113871

Wedge, David E; Klun, Jerome A; Tabanca, Nurhayat; Demirci, Betul; Ozek, Temel; Baser, Kemal Husnu Can; Liu, Zhijun; Zhang, Sui; Cantrell, Charles L; Zhang, Jian

2009-01-28

53

Enhancement of pheochromocytoma nerve cell growth by consecutive fractionization of Angelica gigas Nakai extracts  

PubMed Central

This work was to investigate the effect of flavonoids from Angelica gigas Nakai on the proliferation and differentiation of PC12 cells. Several solvents including hexane, chloroform, ethyl acetate, butanol and water consecutively partitioned. We determined the ethanol crude extract of Angelica gigas Nakai. The hexane fraction was shown to contain the highest number of flavonoids as follows; 21.48 mg/g and the composition of the flavonoids was as follows: 12.24 mg/g of quercetin, 4.39 mg/g of myricetin and 2.58 mg/g of catechin. In addition, this hexane fraction greatly increased both cell growth and outgrowth of the neurite, and whose effects were three times higher than those of the other fractions. The length of the neurites was measured as ca. 110 ?m in adding 50 ?g/mL of the hexane fraction, which was about the same as the case of adding 50 ng/mL of NGF as a positive control. This result indicates that the differentiation of PC12 cells by the addition of the hexane fraction was comparable to the case of adding NGF. The hexane fraction was also determined to prevent apoptosis of PC12 cells by suppressing DNA fragmentation. It is interesting that the mixture of three major flavonoids, quercetin, myricetin and catechin showed stronger activity on, both PC12 cell growth and neuritis outgrowth, than when adding each flavonoid alone. We believe this was due to the synergistic effects of the three flavonoids. The activities of these flavonoids from Angelica gigas Nakai are reported for the first time in this study.

Jeong, Myoung Hoon; Kim, Ji Seon; Zou, Yunyun; Yoon, Chang Soon; Lim, Hye Won; Ahn, Juhee

2010-01-01

54

Isolation, structure and bioactivities of the polysaccharides from Angelica sinensis (Oliv.) Diels: A review.  

PubMed

The root of Angelica sinensis (Oliv.) Diels, a well-known Chinese herbal medicine, has been used historically as a tonic, hematopoietic and anti-inflammatory agent for thousands of years. Modern phytochemistry and pharmacological experiments have proved that polysaccharide is one of the major active ingredients in A. sinensis. It has been demonstrated that A. sinensis polysaccharides had various important biological activities, such as hematopoiesis, immunomodulation, antitumor, antioxidant, radioprotection and hypoglycemic activity. The purpose of the present review is to summarize previous and current references regarding extraction and purification techniques as well as structural characterization and biological activities of A. sinensis polysaccharides. PMID:24750855

Jin, Mingliang; Zhao, Ke; Huang, Qingsheng; Xu, Chunlan; Shang, Peng

2012-07-01

55

beta-secretase inhibitory effects of furanocoumarins from the root of Angelica dahurica.  

PubMed

In the course of screening antidementia agents from natural products, five beta-secretase (BACE1) inhibitors were isolated from the root extract of Angelica dahurica (Umbelliferae). They were identified as furanocoumarins, isoimperatorin (1), imperatorin (2), (+)-oxypeucedanin (3), (+)-byakangelicol (4) and (+)-byakangelicin (5). Among them, compounds 2 and 4 showed significant inhibitory activity against beta-secretase (BACE1) with IC(50) values of 91.8 +/- 7.5 and 104.9 +/- 2.4 microM, respectively. Compounds 1-5 inhibited BACE1 activity in a dose-dependent manner. PMID:20041416

Marumoto, Shinsuke; Miyazawa, Mitsuo

2010-04-01

56

Accumulation of essential oils in relation to root differentiation in Angelica archangelica L.  

PubMed

The accumulation of essential oils in Angelica archangelica subsp. archangelica roots at different developmental stages was investigated through histochemical and chemical analyses. Roots less than 1 mm in diameter showed a primary diarch structure and two primary secretory ducts in the pericycle. These ducts were ephemeral and probably became dysfunctional early on. Oil accumulation was observed only in the secondary secretory ducts formed by cambium activity and located in the secondary phloem. Gas chromatographic analyses revealed that only taproots exceeding 5 mm in diameter contained a high concentration of alpha- and beta-phellandrene, which appreciably influence the oil's aroma. PMID:12685562

Pasqua, G; Monacelli, B; Silvestrini, A

2003-01-01

57

Effect of "jian-pi-zhi-dong decoction" on gamma-aminobutyric Acid in a mouse model of tourette syndrome.  

PubMed

The purpose of this study was to explore the positive effects of Jian-Pi-Zhi-Dong Decoction (JPZDD) on Tourette syndrome (TS) by investigating the expression of gamma-aminobutyric acid (GABA) and its type A receptor (GABAAR) in the striatum of a TS mice model. The model was induced by 3,3'-iminodipropionitrile (IDPN) treatment; then mice were divided into 4 groups (n=22, each); control and IDPN groups were gavaged with saline and the remaining 2 groups were gavaged with tiapride and JPZDD. We recorded the stereotypic behaviors of TS mice and measured the content of GABA in striatum by HPLC and GABAAR expression by immunohistochemistry and real-time PCR. Our results showed that JPZDD inhibited the abnormal behaviors of TS model mice and decreased GABA levels and GABAAR protein and mRNA expression in the striatum of TS model mice. In brief, the mechanism by which JPZDD alleviates TS symptoms may be associated with GABAAR expression downregulation in striatum which may regulate GABA metabolism. PMID:24812567

Zhang, Wen; Yu, Wenjing; Wang, Daohan; Wei, Li; Lee, Minkyoung; Wang, Sumei

2014-01-01

58

Effect of "Jian-Pi-Zhi-Dong Decoction" on Gamma-Aminobutyric Acid in a Mouse Model of Tourette Syndrome  

PubMed Central

The purpose of this study was to explore the positive effects of Jian-Pi-Zhi-Dong Decoction (JPZDD) on Tourette syndrome (TS) by investigating the expression of gamma-aminobutyric acid (GABA) and its type A receptor (GABAAR) in the striatum of a TS mice model. The model was induced by 3,3?-iminodipropionitrile (IDPN) treatment; then mice were divided into 4 groups (n=22, each); control and IDPN groups were gavaged with saline and the remaining 2 groups were gavaged with tiapride and JPZDD. We recorded the stereotypic behaviors of TS mice and measured the content of GABA in striatum by HPLC and GABAAR expression by immunohistochemistry and real-time PCR. Our results showed that JPZDD inhibited the abnormal behaviors of TS model mice and decreased GABA levels and GABAAR protein and mRNA expression in the striatum of TS model mice. In brief, the mechanism by which JPZDD alleviates TS symptoms may be associated with GABAAR expression downregulation in striatum which may regulate GABA metabolism.

Zhang, Wen; Yu, Wenjing; Wei, Li; Lee, Minkyoung; Wang, Sumei

2014-01-01

59

Antitussive principles of Glycyrrhizae radix, a main component of the Kampo preparations Bakumondo-to (Mai-men-dong-tang).  

PubMed

We attempted to elucidate the antitussive principles of Glycyrrhizae radix, a main component of Bakumondo-to (Mai-men-dong-tang). Although the 50% methanol-eluted fraction (100 mg/kg, p.o.) caused a more than 60% reduction in the number of capsaicin-induced coughs, neither the water-eluted nor 100% ethanol-eluted fractions of water extract of G. radix had antitussive effects. The water extract of G. radix contained high levels of liquiritin, liquiritin apioside, isoliquiritin, isoliquiritin apioside and glycyrrhizin. On the other hand, the 50% methanol-eluted fraction contained mainly liquiritin and liquiritin apioside, but not the other compounds. Liquiritin apioside (3-30 mg/kg, p.o.), but not liquiritin, isoliquiritin, isoliquiritin apioside or glycyrrhizin, dose-dependently inhibited the number of coughs. Methysergide, a serotonin receptor antagonist, antagonized the antitussive effect of liquiritin apioside. However, the antitussive effect of liquiritin apioside was not antagonized by naloxone. Pretreatment with glibenclamide (3 mg/kg, i.p.), an ATP-sensitive potassium channel blocker, also significantly reduced the antinociceptive effect of liquiritin apioside. These results suggest that G. radix contains a potent antitussive compound, liquilitin apioside, whose antitussive effect may depend on both peripheral and central mechanisms. PMID:12782198

Kamei, Junzo; Nakamura, Rie; Ichiki, Hiroyuki; Kubo, Masayoshi

2003-05-23

60

A Study of the Phaonia angelicae Group (Diptera: Muscidae), with Descriptions of Six New Species from China  

PubMed Central

The P. angelicae group from China was studied, and six new species are described: P. hanmiensis, n. sp., P. nanlingensis, n. sp., P. reduncicauda, n. sp., P. spargocerca, n. sp., P. subincana, n. sp., and P. varimargina, n. sp. A key to the identification of males of the 40 Chinese species is given.

Xue, Wan-Qi; Zhang, Xiang

2013-01-01

61

Simultaneous quantification of six main active constituents in Chinese Angelica by high-performance liquid chromatography with photodiode array detector  

PubMed Central

Background: Angelica sinensis is a famous traditional Chinese medicinalherb, which is predominantly used in the treatment of gynecological conditions. It is the first report for the simultaneous determination of six major active components in Chinese Angelica, which is important for quality control. Objective: A validated HPLC-PAD method was first developed to evaluate the quality of crude and processed Radix Angelica through simultaneous determination of six bioactive compounds, namely ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, Z/E-ligustilide and Z/E-butylidenephthalide. Materials and Methods: Samples were separated on a Xtimate™C18 column (250 × 4.6 mm, 5 ?m) and detected by PAD. Mobile phase was composed of (A) aqueous phosphoric acid (0.02%, v/v) and (B) acetonitrile (MeCN) (including 10% tetrahydrofuran, v/v) using a gradient elution. Analytes were performed at 30°C with a flow rate of 1.0 mL/min. Results: All calibration curves showed good linear regression (r2 ? 0.9963) within the tested ranges, and the recovery of the method was in the range of 91.927–105.859%. Conclusion: The results demonstrate that the developed method is accurate and reproducible and could be readily utilized as a suitable quality control method for the quantification of Radix Angelica.

Ying, Li; Si-Wang, Wang; Hong-Hai, Tu; Wei, Cao

2013-01-01

62

Pre-Sowing Treatments to Improve Seed Germination in Angelica glauca Edgew, an Endangered Medicinal Herb of the Western Himalayas  

Microsoft Academic Search

Angelica glauca (Apiaceae), endemic to the Himalaya, is an endangered medicinal herb for which, beside in situ conservation, ex situ cultivation is recommended to overcome poor seed germination that limits natural reproduction in the species. To determine the germination potential of the species, the germination of seeds collected from several populations were measured. Germination in seeds treated with KNO3 and

Rajiv K. Vashistha; B. P. Nautiyal; M. C. Nautiyal

2009-01-01

63

Analysis of the monosaccharide components in Angelica polysaccharides by high performance liquid chromatography.  

PubMed

An analytical method of on-line high performance liquid chromatography (HLPC) was developed to simultaneously separate and identify the monosaccharide composition of three Angelica polysaccharide fractions (APF), named APF1, APF2 and APF3. In this method, APF were hydrolyzed into component monosaccharides and subsequently labeled with 1-phenyl-3-methyl-5-pyrazolone (PMP), and then the labeled monosaccharide derivatives were separated by a reverse-phase C18 column and monitored by UV absorbance at 250 nm. The results showed that nine monosaccharide derivatives have been well separated by HPLC under optimized conditions and the composition analysis of monosaccharides from APF samples could be achieved using acid hydrolysis and a set of monosaccharide standards. With this method, the within-day and day to day precisions of the composition determinations were 3.41-4.87% and 3.12-4.93% (RSD), respectively. The method was successfully applied to the determination of the component monosaccharides of Angelica polysaccharides. PMID:16270574

Yang, Xingbin; Zhao, Yan; Wang, Qingwei; Wang, Haifang; Mei, Qibing

2005-10-01

64

14-Methylpentadecano-15-lactone (muscolide): a new macrocyclic lactone from the oil of Angelica archangelica L.  

PubMed

The chemical composition of seed and root oils from Angelica archangelica L. was investigated. Analyses were performed by GC/MS and GC using two columns of different polarities (polyethylene glycol (DB-Wax) and 5% phenyl/95% polydimethylsiloxane (HP-5)), for the separation of several co-eluting components. A total of 58 compounds were identified, accounting for 96.3% (seed) and 93.5% (root) of the oils, respectively. A high content of beta-phellandrene (74.7%) was found in Angelica seed oil. Root oil contained a larger amount of macrocyclic lactones (1.3%) in comparison to the seed oil (0.4%). Different harvest dates produced only slight changes in the root-oil composition. In root oil harvested in summer, the beta-phellandrene content increased by ca. 36%, but no significant changes in the relative compositions of other components were observed. Fresh root oils were collected in five fractions (constant time intervals) during steam distillation (see Table). The highest-boiling fraction contained 9.3% of macrocyclic lactones such as tridecano-13-lactone (5.0%), 12-methyltridecano-13-lactone (0.4%), tetradecano-14-lactone (0.1%), pentadecano-15-lactone (3.5%), 14-methylpentadecano-15-lactone (1; trace), hexadecano-16-lactone (trace), and heptadecano-17-lactone (0.2%). This is the first report of the occurrence of 14-methylpentadecano-15-lactone (muscolide; 1) in a natural product. PMID:17191826

Lopes, Daíse; Strobl, Herbert; Kolodziejczyk, Paul

2004-12-01

65

Selenylation modification can enhance immune-enhancing activity of Chinese angelica polysaccharide.  

PubMed

On the basis of previous test that selenizing Chinese angelica polysaccharides (sCAPs) with stronger immune-enhancing activity in vitro were picked out, the immune-enhancing activity in vivo of three sCAPs, sCAP2, sCAP6 and sCAP8, at high and low dosage were compared taking the unmodified Chinese angelica polysaccharide (CAP) as control by determination of peripheral lymphocyte proliferation, serum antibody titer, IFN-? and IL-6 contents in chicken vaccinated with Newcastle Disease vaccine. The results showed that three sCAPs at suitable dosage could significantly promote lymphocyte proliferation, enhance serum antibody titer, IFN-? and IL-6 contents as compared with unmodified CAP, sCAP2 at low dosage possessed the strongest action. These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of CAP, sCAP2 possessed the best efficacy and would be as a component drug of new-type immunoenhancer. PMID:23618257

Qin, Tao; Chen, Jin; Wang, Deyun; Hu, Yuanliang; Zhang, Jing; Wang, Mi; Qiu, Shulei; Gao, Zhenzhen; liu, Rongrong; Yu, Yun; Huang, Yee; Wang, Qiaochu; Wang, Qiaoxiu

2013-06-01

66

Characterization and antioxidative activities of polysaccharide in Chinese angelica and its processed products.  

PubMed

Five polysaccharides from unprocessed Chinese angelica (UCAP), parched one with alcohol (ACAP), soil (SCAP), sesame oil (OCAP) and parched into charred (CCAP) were extracted and purified. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), compositions were analyzed by gas chromatography-mass spectrometry (GC-MS) and antioxidative activities were compared by determining MDA contents and SOD activities of liver tissue in mice damaged with CCl4 after gavage. The results showed that the FT-IR spectra of CCAP and OCAP displayed lower transmittance at around 1050cm(-1) in comparison with that of UCAP. Five polysaccharides were all composed of rhamnose, arabinose, mannose, glucose and galactose. In CCAP, ACAP, OCAP and SCAP, the proportions of arabinose were significantly increased in comparison with that of UCAP. The SOD activities in CCAP and SCAP groups were significantly enhanced, and MDA contents in CCAP, OCAP and SCAP groups were significantly decreased as compared with UCAP group. This indicated that processing could change the structure, composition and enhance antioxidative activity of polysaccharide in Chinese angelica, and CCAP possessed the strongest antioxidative activity. PMID:24680904

Ji, Peng; Wei, Yanming; Xue, Wenxin; Hua, Yongli; Zhang, Man; Sun, Hongguo; Song, Zhixue; Zhang, Ling; Li, Jinxia; Zhao, Haifu; Zhang, Wenquan

2014-06-01

67

Authentication of Angelica anomala Avé-Lall cultivars through DNA barcodes.  

PubMed

Angelica anomala Avé-Lall (Chuanbaizhi in Chinese) is an important medicinal plant which can be used in traditional Chinese medicines; however, there are no authentic and universal methods to differentiate this Sichuan famous-region drug of A. anomala from a large number of non-famous-region and false drugs. It has been demonstrated that DNA barcoding is a molecular diagnostic method for species identification, which uses a single standardized DNA fragment. In this study, we tested five DNA barcoding candidates (matK, ITS, ITS2, rbcL, and psbA-trnH), and we found that ITS was the best candidate to authenticate the famous-region drug of A. anomala. Moreover, through comparative analysis of these five DNA barcodes between A. anomala and Angelica dahurica, we found that ITS had the most and ITS2 had more variable regions, but the psbA-trnH, rbcL, and matK regions were identical. Hence, we suggest ITS as the DNA barcoding to identify A. anomala and A. dahurica. Moreover, we are determined to adopt the A. anomala as the accurate Latin name of Chuanbaizhi. PMID:22397381

He, Yang; Hou, Pei; Fan, Gang; Song, Zhen; Arain, Saima; Shu, Hao; Tang, Ce; Yue, Qinghong; Zhang, Yi

2012-04-01

68

[History and the present of cultivation and production of Angelica root, Yamato-Toki, in Japan].  

PubMed

Japanese Angelica root, Toki in Japanese, is prepared from the roots of cultivated Angelica acutiloba or A. acutiloba var. sugiyamae. Since Toki has been frequently used as a crude drug in traditional Chinese formulations, the stable supply and quality of Toki are essential issues in Japanese clinical practice. To clarify the historical and present conditions of Toki, a historical survey on herbal books and a field investigation on the cultivation condition of A. acutiloba in the Fuki area (Wakayama Prefecture) were carried out. From the present historical survey, it was proven that Yamato-Toki produced in the Yamato area, an old local area including the current Nara and Wakayama prefectures in Japan, had been considered to be superior merchandise. It was also proven that a special processing method to prevent flower stalk growth ("Mekuri" in Japanese) is an original method different from Chinese methods. From the present field investigation, it was also proven that the traditional transplant operation of the second year has been handed down since the 1930s and that washing roots in hot water ("Yumomi" in Japanese) is an original method of preparing Yamato-Toki. Toki is one of the precious crude drugs cultivated and prepared in Japan. The present study may help to pass on traditional cultivation culture and contribute to an expansion in the volume of Yamato-Toki produced in Japan. PMID:20527290

Fukuda, Kozo; Murata, Kazuya; Matsuda, Hideaki; Tani, Tadato

2009-01-01

69

Effects of auxins on growth and scopoletin accumulation in cell suspension cultures of Angelica archangelica L.  

PubMed

Scopoletin is a coumarin possessing many interesting biological effects, e.g., spasmolytic, anti-inflammatory, antimutagenic, antioxidant, antifungal, apoptosis-inducing, antiproliferative, acetylcholinesterase-inhibitory, and hypouricemic activities. Plant tissue cultures represent a promising alternative source of valuable plant-derived substances. A number of physical and chemical factors influence the cell growth and secondary metabolite biosynthesis in plant tissue cultures. The mechanism of their action is not completely understood. Besides other factors, plant growth regulators and light conditions play an important role. Effects of four auxins (2,4-dichlorophenoxyacetic acid, 2,4-D, alpha-naphthaleneacetic acid, NAA, beta-indoleacetic acid, IAA or beta-indolebutyric acid, IBA) at four concentrations (0.2, 2, 10 or 20 mg/l) on the culture growth and accumulation of scopoletin in the medium were tested in Angelica archangelica cell suspension cultures cultured under continuous light or in the dark. The highest culture growth was achieved with 2 mg/l 2,4-D, and 10 mg/l IAA. The best scopoletin levels were obtained with 0.2 mg/l 2,4-D, 2 mg/l 2,4-D, 10 mg/l NAA, and 20 mg/l IAA. The effects of light conditions were less marked than those of auxins and their concentrations in influencing both the cell growth and scopoletin accumulation in Angelica archangelica cell suspension cultures. The changes brought about by auxins were modified by light conditions. PMID:18383919

Siatka, T; Kasparová, M

2008-01-01

70

Antidiabetic activities of chalcones isolated from a Japanese Herb, Angelica keiskei.  

PubMed

Diabetes mellitus is a chronic disease that is characterized by hyperglycemia caused by insufficient insulin action. We have explored the edible ingredients from folk medicines in Japan that contain substances complementing insulin action, such as the induction of adipocyte differentiation and the enhancement of glucose uptake. We eventually found that the ethanol extract from a Japanese herb "Ashitaba", Angelica keiskei, contained two major chalcones of 4-hydroxyderricin (4-HD) and xanthoangelol that showed strong insulin-like activities via a pathway independent of the peroxisome proliferator-activated receptor-gamma activation. The 4-HD especially showed the preventive effects on the progression of diabetes in genetically diabetic KK-Ay mice. PMID:17583349

Enoki, Tatsuji; Ohnogi, Hiromu; Nagamine, Kinuko; Kudo, Yoko; Sugiyama, Katsumi; Tanabe, Masashige; Kobayashi, Eiji; Sagawa, Hiroaki; Kato, Ikunoshin

2007-07-25

71

Six new chalcones from Angelica keiskei inducing adiponectin production in 3T3-L1 adipocytes.  

PubMed

Angelica keiskei (Ashitaba in Japanese), a traditional herb in Japan, contains abundant prenylated chalcones. It has been reported that the chalcones from A. keiskei showed such bioactivities as anti-bacterial, anti-cancer and anti-diabetic effects. Xanthoangelol, 4-hydroxyderricin and six new chalcones were isolated in this study from an ethanol extract of A. keiskei by octadecyl silyl (ODS) and silica gel chromatography, and identified by 1D- and 2D-nuclear magnetic resonance (NMR) and high-resolution mass spectrometric analyses. The chalcones from A. keiskei markedly increased the expression of the adiponectin gene and the production of adiponectin in 3T3-L1 adipocytes. These results suggest that the chalcones from A. keiskei might be useful for preventing the metabolic syndrome. PMID:22738967

Ohnogi, Hiromu; Kudo, Yoko; Tahara, Kenichi; Sugiyama, Katsumi; Enoki, Tatsuji; Hayami, Shoko; Sagawa, Hiroaki; Tanimura, Yuko; Aoi, Wataru; Naito, Yuji; Kato, Ikunoshin; Yoshikawa, Toshikazu

2012-01-01

72

Can Rhizoma Chuanxiong replace Radix Angelica sinensis in the traditional Chinese herbal decoction Danggui Buxue Tang?  

PubMed

Herein, we test the hypothesis that a member of a formulated Chinese herbal decoction cannot be replaced by another herb. Danggui Buxue Tang (DBT) is being used as an example for illustration: this is a traditional decoction containing Radix Astragali (RA) and Radix Angelicae Sinensis (RAS) in a weight ratio of 5 to 1. Rhizoma Chuanxiong (RC) and RAS are two chemically very similar herbs but with a distinct function. Following the preparation method of DBT, a herbal decoction, namely Chuanxiong Buxue Tang (CBT), was created, which contained RA and RC in a weight ratio of 5 to 1. The two decoctions, DBT and CBT, were compared in parallel regarding their chemical and biological properties. In all the tested parameters, DBT showed superior properties, both chemically and biologically, to that of CBT. The current results reveal the uniqueness of Chinese herbal decoctions that require a well-defined formulation, which is indispensable for its specific composition. PMID:19204892

Li, Winnie Z M; Li, Jun; Bi, Cathy W C; Cheung, Anna W H; Huang, Wen; Duan, Ran; Choi, Roy C Y; Chen, Ivy S Y; Zhao, Kui J; Dong, Tina T X; Duan, Jin A; Tsim, Karl W K

2009-05-01

73

Antivirus and immune enhancement activities of sulfated polysaccharide from Angelica sinensis.  

PubMed

This study is to synthesize sulfated Angelica polysaccharides (APSs) and investigate the activity of one of the sulfated derivatives APS-1 on murine leukemia virus in vivo. Six sulfated derivatives with degree of sulfation ranging from 0.68 to 1.91 were obtained. And the virus replication was inhibited by APS-1 at the dose of 10 and 30 mg/kg (26% and 30% inhibition respectively). Furthermore, both the percentage of CD4(+) cells and CD4(+)/CD8(+) ratio in peripheral blood cells were significantly enhanced by APS-1 at 3-30 mg/kg. In addition, the reduced thymus/body weight index by murine leukemia virus infection was increased by ASP-1 in a dose dependent manner. These results suggest that APS-1 could not only inhibit virus replication, but also improve the immune function. APS-1 may be a potential new and better antiviral drug. PMID:22155400

Yang, Tiehong; Jia, Min; Zhou, Siyuan; Pan, Feng; Mei, Qibing

2012-04-01

74

Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity.  

PubMed

Chinese angelica polysaccharide (CAP) was extracted by water decoction and ethanol precipitation, purified through eliminating protein by Sevage method and column chromatography of Sephadex G-200, then selenizingly modified by nitric acid-sodium selenite method according to L(9)(3(4)) orthogonal design of three-factors, the usage amount of sodium selenite, reaction temperature and reaction time, at three level to obtain nine selenizing CAPs, sCAP(1)-sCAP(9). Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay taking the non-modified CAP as control. The results showed that selenylation modification could significantly enhance the immune-enhancing activity of CAP, sCAP(2) presented best effect and the optimal modification conditions were 200mg of sodium selenite for 500 mg of CAP, the reaction temperature of 70 °C and the reaction time of 6 h. PMID:23218348

Qin, Tao; Chen, Jin; Wang, Deyun; Hu, Yuanliang; Wang, Mi; Zhang, Jing; Nguyen, The Luong; Liu, Cui; Liu, Xu

2013-01-30

75

Extraction optimization of Angelica sinensis polysaccharides and its antioxidant activity in vivo.  

PubMed

Extraction of Angelica sinensis polysaccharides (ASP) was optimized by the utilization of response surface methodology, RSM. Through the analysis, extraction time and water/solid were found to be the most significant factors. Based on contour plots and variance analysis, optimum operational conditions for maximizing polysaccharides yield (5.6%) were found to be extraction time 130 min, water/solid 5, and extraction number 5. A. sinensis polysaccharides (150 and 300 mg/kg) were administered for 15 days. The hepatoprotective activity was assessed using various biochemical parameters. Serum aspartate aminotransferase (AST), alanine aminotransfere (ALT) and alkaline phosphatase (ALP) levels were significantly restored toward normalization by the extracts (150 and 300 mg/kg body weight). ASP (150 and 300 mg/kg body weight) significantly increased the levels of antioxidant enzymes. It can be concluded that ASP possesses significant protective effect against hepatotoxicity induced by carbon tetrachloride (CCl4). This protective effect appears due to ASP antioxidant properties. PMID:23544518

Yu, Fang; Li, Huali; Meng, Yu; Yang, Di

2013-04-15

76

Antiproliferative effect of furanocoumarins from the root of Angelica dahurica on cultured human tumor cell lines.  

PubMed

A bioassay-guided fractionation of the root extract of Angelica dahurica (Umbelliferae) led to the isolation of six furanocoumarins as active ingredients responsible for the antitumoral property. The hexane soluble part of the extract demonstrated a significant inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nervous system) and HCT-15 (colon) in vitro, whereas the remaining water soluble part exhibited poor inhibition. Intensive investigation of the hexane soluble part of the extract yielded six furanocoumarins, i.e. isoimperatorin, cnidicin, imperatorin, oxypeucedanin, byakangelicol, oxypeucedanin hydrate, all of which exhibited a significant inhibition on cell proliferation in a dose-dependent manner. PMID:17143927

Kim, Young-Kyoon; Kim, Young Sup; Ryu, Shi Yong

2007-03-01

77

Furocoumarin derivatives from radix Angelicae dahuricae and their effects on RXR? transcriptional regulation.  

PubMed

A novel furocoumarin derivative named oxyalloimperatorin (1), together with seventeen furocoumarins 2-18 were isolated from the radix of Angelica dahurica. The chemical structure of new metabolite was characterized by analysis of IR, NMR, and HR-ESI-MS spectroscopic data. Among the isolated compounds, 13, 16, and 18 (each at 20 ?M) could significantly promote the gene transcriptional function of nuclear receptor RXR?. While 7-9, 13, 14, and the new structure 1 (each at 20 ?M) showed significant reduction in RXR? gene transcriptional activities induced by 9-cis-retinoid acid. The findings indicated that these furocoumarin skeleton derivatives might hold beneficial effects on many intractable diseases, such as cancer and metabolic diseases, due to their potential activities on regulating the transcriptional activation function of RXR?. PMID:21792151

Liu, Dong-Ping; Luo, Qiang; Wang, Guang-Hui; Xu, Yang; Zhang, Xiao-Kun; Chen, Quan-Cheng; Chen, Hai-Feng

2011-01-01

78

Inhibitory effects of furanocoumarins isolated from the roots of Angelica dahurica on prostaglandin E2 production.  

PubMed

We have isolated five furanocoumarins, byakangelicin, phellopterin, imperatorin, isoimperatorin, and oxypeucedanin methanolate, from the roots of Angelica dahurica (Umbelliferae) and prepared five semi-synthesized compounds by the partial reduction of each isolated furanocoumarin, and the effects of these compounds on lipopolysaccharide (LPS)-induced prostaglandin E2 (PGE2 ) production in rat peritoneal macrophages were examined. Among these compounds, imperatorin showed the most potent inhibitory activity on the LPS-induced PGE2 production. It also inhibited the LPS-induced expressions of cyclooxygenase (COX)-2 and microsomal prostaglandin E synthase (mPGES). These findings suggest that the inhibitory effect of furanocoumarins on the LPS-induced PGE2 production is due to the inhibition of the expression of COX-2 and mPGES. PMID:12802720

Ban, Hyun Seung; Lim, Soon Sung; Suzuki, Katsuya; Jung, Sang Hoon; Lee, Sanghyun; Lee, Yeon Sil; Shin, Kuk Hyun; Ohuchi, Kazuo

2003-05-01

79

Geographical variation of the furanocoumarin composition of the fruits of Icelandic Angelica archangelica.  

PubMed

Angelica archangelica fruits were collected from 64 locations around Iceland and analysed for furanocoumarins by high-performance liquid chromatography. The average furanocoumarin content was found to be 22.5 mg/g, ranging from 14.0 to 31.6 mg/g. Whereas imperatorin was the main compound in all samples, the order of other compounds was highly diverse. Considerable differences were observed between individuals from the same location and between neighbouring locations. However, strong geographical impact was observed on the composition, with isoimperatorin and bergapten being more pronounced in South Iceland, and oxypeucedanin and an unidentified compound being more pronounced in North Iceland and absent in many samples from South Iceland. PMID:22486035

Sigurdsson, Steinthor; Jonsdottir, Sigridur; Gudbjarnason, Sigmundur

2012-01-01

80

Choice of solvent in the extraction of Angelica archangelica roots with reference to calcium blocking activity.  

PubMed

Twenty solvents were tested in the extraction of compounds from the roots of Angelica archangelica L. (Apiaceae), and the calcium-antagonistic activity of the extracts was investigated. Special attention was paid to the physical and chemical properties of the solvents and their extraction abilities. The calcium antagonistic effect of the extracts was investigated by measuring the inhibition of depolarization-induced Ca2+ uptake in rat pituitary GH4C1 cells. The criteria used in determining the best solvents for the extraction were the yield and the biological activity of the extract, as well as the amount of nonpolar compounds in the extract. The final criterion used in selecting the solvent was its usability with reference to boiling point, chemical interactions (e.g. methylation), etc. Chloroform was found to be the best solvent for the extraction of nonpolar, biologically active compounds from the roots of A. archangelica. PMID:1529031

Härmälä, P; Vuorela, H; Törnquist, K; Hiltunen, R

1992-04-01

81

Inhibition of acetylcholinesterase by extracts and constituents from Angelica archangelica and Geranium sylvaticum.  

PubMed

The aim of this study was to explore the acetylcholinesterase (AChE) inhibition of several Icelandic medicinal herbs. Ethanolic extracts of Angelica archangelica seeds and the aerial parts of Geranium sylvaticum proved effective, with IC50 values of 2.20 mg/ml and 3.56 mg/ml, respectively. The activity of imperatorin and xanthotoxin from A. archangelica was measured. Xanthotoxin proved much more potent than imperatorin, with an IC50 value of 155 microg/ml (0.72 mM) but that for imperatorin was above 274 microg/ml (1.01 mM). However, furanocoumarins seem to have a minor part in the total activity of this extract. Synergistic interaction was observed between the extracts of A. archangelica and G. sylvaticum. Several medicinal herbs (Achillea millefolium, Filipendula ulmaria, Thymus praecox and Matricaria maritima) did not show AChE inhibitory activity. PMID:18069242

Sigurdsson, Steinthor; Gudbjarnason, Sigmundur

2007-01-01

82

[Effect of auxins on production of coumarin in a suspension culture of Angelica archangelica L].  

PubMed

The paper examined the effect of selected auxins (2,4-dichlorophenoxyacetic acid, alpha-naphthalene-acetic acid, beta-indoleacetic acid, beta-indoleburytic acid; each in four concentrations--0.2, 2, 10, and 20 mg/l) on the production of coumarins in the suspension culture of Angelica archangelica L. cultinated in the dark and under permanent lighting(3500 lux). The effect of the light regimen is, in comparison with auxins, less marked--the content of coumarins is mostly comparable both under permanent lighting and in the dark. The highest coumarin content was achieved with the use of alpha-naphthalene-acetic acid in a concentration of 0.2 mg/l with cultivation in the dark. PMID:12924070

Siatka, T; Kasparová, M

2003-07-01

83

Anti-anxiety Activity of Methanolic Extracts of Different Parts of Angelica archangelica Linn.  

PubMed

Angelica archangelica Linn.is a herb distributed in tropical and subtropical regions of the world. In Indian and Chinese system of medicine, it is used for nervous disorders and cerebral diseases. Previously the aqueous extract of the A. archangelica was evaluated for anxiolytic activity and was found to have significant potential for the same. The present study is aimed to evaluate the anxiolytic activity of methanol extract of root (MER), stem (MES), leaf (MEL), fruit (MEF) and whole plant (MEW) of Angelica archangelica Linn. All the extracts (MER, MES, MEL, MEF and MEW) were evaluated for anxiolytic effects using elevated plus maze test (EPM) model in rats. Methanol extracts of different parts of A.archangelica had increased number of entries and time spent in open arms while they decreased the number of entries and duration of time spent in closed arm of the EPM. In a similar fashion, the diazepam increased the percentage of time spent and percentage of arm entries in the open arms (*P <0.05, **P <0.01). Whole plant and the root had the maximum, leaf and fruits showed intermediate, while stem had the least anxiolytic activity (*P <0.05, **P <0.01) in EPM (Figure 1-5). The head dip count in DZ, SMR400, SML400, SMF400 and SMW400 in open arm are significantly shown in Table 1. The DZ, SMF400 and SMW did not show the fecal bolus while other groups were reduced the fecal bolus significantly (**P <0.01) as compared to control (Table 1). Whole plant and leaf showed the most, root and fruit the intermediate and stem the least anxiolytic activity (**P <0.01) in EPM. PMID:24716138

Kumar, Dinesh; Bhat, Zulfiqar Ali

2012-07-01

84

Anti-anxiety Activity of Methanolic Extracts of Different Parts of Angelica archangelica Linn.  

PubMed Central

Angelica archangelica Linn.is a herb distributed in tropical and subtropical regions of the world. In Indian and Chinese system of medicine, it is used for nervous disorders and cerebral diseases. Previously the aqueous extract of the A. archangelica was evaluated for anxiolytic activity and was found to have significant potential for the same. The present study is aimed to evaluate the anxiolytic activity of methanol extract of root (MER), stem (MES), leaf (MEL), fruit (MEF) and whole plant (MEW) of Angelica archangelica Linn. All the extracts (MER, MES, MEL, MEF and MEW) were evaluated for anxiolytic effects using elevated plus maze test (EPM) model in rats. Methanol extracts of different parts of A.archangelica had increased number of entries and time spent in open arms while they decreased the number of entries and duration of time spent in closed arm of the EPM. In a similar fashion, the diazepam increased the percentage of time spent and percentage of arm entries in the open arms (*P <0.05, **P <0.01). Whole plant and the root had the maximum, leaf and fruits showed intermediate, while stem had the least anxiolytic activity (*P <0.05, **P <0.01) in EPM (Figure 1-5). The head dip count in DZ, SMR400, SML400, SMF400 and SMW400 in open arm are significantly shown in Table 1. The DZ, SMF400 and SMW did not show the fecal bolus while other groups were reduced the fecal bolus significantly (**P <0.01) as compared to control (Table 1). Whole plant and leaf showed the most, root and fruit the intermediate and stem the least anxiolytic activity (**P <0.01) in EPM.

Kumar, Dinesh; Bhat, Zulfiqar Ali

2012-01-01

85

Comparison of the toxicity of aqueous and ethanol fractions of Angelica keiskei leaf using the eye irritancy test  

PubMed Central

To determine whether aqueous and ethanol fractions of the Angelica keiskei leaf exert toxicity when used for cosmetic purposes, we performed the acute eye irritancy test. Animals were treated with sample fractions (100 mg/dose) according to standard procedure guidelines. No significant changes or damage was detected in the fraction-treated groups in terms of ocular lesions in the cornea, the size of the cornea with turbidity, swelling of the eyelid and emission discharge. However, sodium dioctyl sulfosuccinate, a positive control, induced severe toxic symptoms. Thus, aqueous and ethanol fractions of Angelica keiskei do not appear to induce acute toxicity in the eye lens, as assessed from anatomical and pathological observations in the rabbit eye. Our results collectively suggest that aqueous and ethanol fractions show promise as cosmetic ingredients that do not cause eye toxicity.

SON, HYEONG-U; YOON, EUN-KYUNG; CHA, YONG-SOO; KIM, MIN-A; SHIN, YONG-KYU; KIM, JONG-MYUNG; CHOI, YONG-HEE; LEE, SANG-HAN

2012-01-01

86

Angelica sinensis isolate SBD.4: composition, gene expression profiling, mechanism of action and effect on wounds, in rats and humans.  

PubMed

This report characterizes an aqueous isolate (SBD.4) of one of the most broadly used Chinese medicinal herbs, Angelica sinensis, from the perspective of its application in skin and wound care. SBD.4 has been chemically defined and was found to increase the strength of healed wounds in retired breeder (older) rats. Furthermore, the mechanism of action of this Angelica sinensis isolate was tested in the zebrafish angiogenesis model, and in human skin substitutes by DNA microarray, revealing a bioactivity profile consistent with skin repair and regeneration. When combined with several types of wound dressings, SBD.4 increased type I collagen production in human dermal fibroblasts, and when formulated in nanosilver hydrocolloid dressing, it was found effective in chronic ulcer management in humans, demonstrating that botanical high-tech wound dressings can be successfully developed to improve the treatment of chronic lesions in humans. PMID:22146555

Zhao, Hui; Deneau, Joel; Che, Ginny O L; Li, Shang; Vagnini, Frederic; Azadi, Parastoo; Sonon, Roberto; Ramjit, Ravi; Lee, Simon M Y; Bojanowski, Krzysztof

2012-01-01

87

Chromatographic fingerprint analysis and characterization of furocoumarins in the roots of Angelica dahurica by HPLC/DAD/ESI-MSn technique.  

PubMed

A high performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry (HPLC/DAD/ESI-MSn) method was used for the chromatographic fingerprint analysis and characterization of furocoumarins in the roots of Angelica dahurica for the first time. Nine "common peaks" were identified by comparing with the retention time, UV and MS spectra of reference furocoumarins. The software "Similarity Evaluation System for Chromatographic Fingerprint of TCM" was used to evaluate the similarities of 13 batches of Baizhi samples collected from Henan, Zhejiang, Sichuan and Anhui provinces of China. The results indicated that the samples from different batches had similar HPLC fingerprints, and the method could be applied for the quality control of the roots of Angelica dahurica. In addition, a total of 20 furocoumarins were identified or tentatively characterized by HPLC/DAD/ESI-MSn technique, and their fragmentation patterns in an electrospray ion trap mass spectrometer were also summarized. PMID:18436412

Kang, Jie; Zhou, Lei; Sun, Jianghao; Han, Jian; Guo, De-An

2008-08-01

88

Bioactivity-guided fractionation for the butyrylcholinesterase inhibitory activity of furanocoumarins from Angelica archangelica L. roots and fruits.  

PubMed

Isolation and identification of the inhibitors of butyrylcholinesterase (BChE), obtained from the extracts of roots and fruits of Angelica archangelica L., are reported. Our results confirmed the weak inhibitory effect of Angelica roots on acetylcholinesterase activity. BChE inhibition was much more pronounced at a concentration of 100 ?g/mL for hexane extracts and attained a higher rate than 50%. The TLC bioautography guided fractionation and spectroscopic analysis led to the isolation and identification of imperatorin from the fruit's hexane extract and of heraclenol-2'-O-angelate from the root's hexane extract. Both compounds showed significant BChE inhibition activity with IC(50) = 14.4 ± 3.2 ?M and IC(50) = 7.5 ± 1.8 ?M, respectively. Only C8-substituted and C5-unsubstituted furanocoumarins were active, which could supply information about the initial structures of specific BChE inhibitors. PMID:21786787

Wszelaki, Natalia; Paradowska, Katarzyna; Jamróz, Marta K; Granica, Sebastian; Kiss, Anna K

2011-09-14

89

Decursin and decursinol angelate from Angelica gigas Nakai induce apoptosis via induction of TRAIL expression on cervical cancer cells  

Microsoft Academic Search

Aim of the studyThe root of Angelica gigas Nakai (Korean “Dang-Gui”) has been used to treat female afflictions and anemia in traditional oriental herbal medicine since ancient times in Korea. The objective of this study is to identify the anti-cancer mechanism induced by A. gigas extract including decursin and decursinol angelate in human cervical cancer cells for scientific evidence regarding

Nam-Hui Yim; Ju Hye Lee; Won-Kyung Cho; Min Chul Yang; Dong Hoon Kwak; Jin Yeul Ma

90

Enhanced accumulation of decursin and decursinol angelate in root cultures and intact roots of Angelica gigas Nakai following elicitation  

Microsoft Academic Search

Angelica gigas root cultures were elicited with various elicitors, including yeast extract, chitin, methyl jasmonate, salicylic acid, and\\u000a copper, with the aim of increasing the production of decursin and decursinol angelate. The treatment of A. gigas root cultures with a combination of yeast extract (2 g l?1) and copper ion (0.5 mM) at the late exponential growth phase increased decursinol angelate accumulation up

Hong Soon Rhee; Hwa-Young Cho; Seok Young Son; Sung-Yong H. Yoon; Jong Moon Park

2010-01-01

91

Use of Sodium Hypochlorite to Enhance Seedling Emergence, Vigor, and Survival of Angelica glauca and Aconitum heterophyllum  

Microsoft Academic Search

Angelica glauca Edgew. and Aconitum heterophyllum Wall., high value, endangered medicinal plants of the Himalayan region, have low germination rates and slow seedling growth. The present study examined the effect of a common seed surface disinfectant, sodium hypochlorite (NaHClO3), on seedling emergence, vigor, and survival of the above species. Seeds of A. glauca soaked (treated) in NaHClO3 for 30 min

Jitendra S. Butola; Hemant K. Badola

2008-01-01

92

Rapid separation and identification of furocoumarins in Angelica dahurica by high-performance liquid chromatography with diode-array detection, time-of-flight mass spectrometry and quadrupole ion trap mass spectrometry.  

PubMed

High-performance liquid chromatography with diode-array detection (HPLC/DAD), time-of-flight mass spectrometry (HPLC/TOFMS) and quadrupole ion trap mass spectrometry (HPLC/QITMS) were used for separation, identification and structural analysis of furocoumarins in Angelica dahurica. Two furocoumarins (imperatorin and isoimperatorin) in Angelica dahurica extract were identified unambiguously by comparing their relative retention times, characteristic ultraviolet information and accurate mass measurement. A formula database of known furocoumarins in Angelica dahurica was established, against which the other 21 furocoumarins were identified effectively based on the accurate extract masses and formulae acquired by HPLC/TOFMS. In order to distinguish the isomers, multi-stage mass spectrometry (MSn, ion trap mass spectrometry) was used. General fragmentation behavior of the furocoumarins in the ion trap mass spectrometer was studied by the two furocoumarin standards, and their fragmentation rules in MS(n) spectra were summarized. These deduced fragmentation rules of furocoumarins were successfully implemented in distinguishing the three groups of isomers in Angelica dahurica by HPLC/QITMS. By using the three different analytical techniques, 23 furocoumarins in Angelica dahurica were tentatively identified within 30 min. Finally, HPLC/TOFMS fingerprints of Angelica dahurica were established by which it can be concluded that a rapid and effective method based on the three analytical techniques for identification of chemical components was established. This can provide help for further quality control of Angelica dahurica and pharmacology mechanism study of furocoumarins in Angelica dahurica. PMID:19530154

Zhang, Hai; Gong, Chungui; Lv, Lei; Xu, Yuanjie; Zhao, Liang; Zhu, Zhenyu; Chai, Yifeng; Zhang, Guoqing

2009-07-01

93

Quantification of ligustilides in the roots of Angelica sinensis and related umbelliferous medicinal plants by high-performance liquid chromatography and liquid chromatography-mass spectrometry.  

PubMed

A reversed phase high-performance liquid chromatographic method was developed for quantifying E-ligustilide (1) and Z-ligustilide (3) in the roots of Angelica sinensis (Oliv.) Diels with confirmation using UV, atmospheric pressure chemical ionisation (APCI) MS and APCI-MS-MS techniques. Based on the UV spectra of compounds 1, E-butylidenephthalide (2), 3 and Z-butylidenephthalide (4), the absorption at 350 nm was chosen as measuring wavelength in which baseline separation of compounds 1 and 3 could be obtained but avoided the interference of compounds 2 and 4. The identity of compounds 1 and 3 in samples were unambiguously determined by the respective quasi-molecular ions ([M+H]+) in APCI-MS. According to the stability data, acetonitrile was chosen for the preparation of standard solutions in order to minimize the isomerization of compound 3. Compounds 1 and 3 were qualitatively and quantitatively analyzed in seven samples of the roots of Angelica sinensis (Oliv.) Diels, Angelica acutiloba Kitagawa, Angelica acutiloba Kitagawa var. sugiyamae Hikino and the rhizome of Ligusticum chuanxiong Hort. Analysis of an extract from a sample root of Angelica gigas Nakai using LC-MS for the first time could not detect the presence of ligustilide in this herb. The overall analytical procedure is rapid and reproducible which is considered suitable for quantitative analysis of large number of samples. PMID:15387176

Lu, Guang-Hua; Chan, Kelvin; Chan, Chi-Leung; Leung, Kelvin; Jiang, Zhi-Hong; Zhao, Zhong-Zhen

2004-08-13

94

An Experimental Novel Study: Angelica sinensis Prevents Epidural Fibrosis in Laminectomy Rats via Downregulation of Hydroxyproline, IL-6, and TGF- ? 1.  

PubMed

With laminectomy being widely accepted as the treatment for lumbar disorders, epidural fibrosis (EF) is a common complication for both the patients and the surgeons alike. Currently, EF is thought to cause recurrent postoperative pain after laminectomy or after discectomy. Angelica sinensis is a traditional Chinese medicine which has shown anti-inflammatory, antifibrotic, and antiproliferative properties. The object of this study was to investigate the effects of Angelica sinensis on the prevention of post-laminectomy EF formation in a rat model. A controlled double-blinded study was conducted in sixty healthy adult Wistar rats that underwent laminectomy at the L1-L2 levels. They were divided randomly into 3 groups according to the treatment method, with 20 in each group: (1) Angelica sinensis treatment group, (2) saline treatment group, and (3) sham group (laminectomy without treatment). All rats were euthanized humanely 4 weeks after laminectomy. The hydroxyproline content, Rydell score, vimentin cells density, fibroblasts density, inflammatory cells density, and inflammatory factors expressions all suggested better results in Angelica sinensis group than the other two groups. Topical application of Angelica sinensis could inhibit fibroblasts proliferation and TGF- ? 1 and IL-6 expressions and prevent epidural scar adhesion in postlaminectomy rat model. PMID:24069047

Zhang, Chao; Kong, Xiaohong; Zhou, Hengxing; Liu, Chang; Zhao, Xuechao; Zhou, Xianhu; Su, Yanhua; Sharma, Hari S; Feng, Shiqing

2013-01-01

95

An ethanolic extract of Angelica gigas improves atherosclerosis by inhibiting vascular smooth muscle cell proliferation  

PubMed Central

The effects of an ethanolic extract of Angelica gigas (EAG) on the vascular smooth muscle cell (VSMC) proliferation and high-cholesterol diet-induced hypercholesterolemia and atherosclerosis were investigated. Rat aortic VSMCs were stimulated with platelet-derived growth factor-BB (25 ng/mL) for the induction of DNA synthesis and cell proliferation. EAG (1-10 µg/mL) significantly inhibited both the thymidine incorporation and cell proliferation in a concentration-dependent manner. Hypercholesterolemia was induced by feeding male New Zealand white rabbits with 0.5% cholesterol in diet for 10 weeks, during which EAG (1% in diet) was given for the final 8 weeks after 2-week induction of hypercholesterolemia. Hypercholesterolemic rabbits exhibited great increases in serum total cholesterol and low-density lipoproteins (LDL) levels, and finally severe atheromatous plaque formation covering 28.4% of the arterial walls. EAG significantly increased high-density lipoproteins (HDL), slightly decreased LDL, and potentially reduced the atheroma area to 16.6%. The results indicate that EAG attenuates atherosclerosis not only by inhibiting VASC proliferation, but also by increasing blood HDL levels. Therefore, it is suggested that EAG could be an alternative or an adjunct therapy for the improvement of hypercholesterolemia and atherosclerosis.

Jang, Ja Young; Kim, Jihyun; Cai, Jingmei; Kim, Youngeun; Shin, Kyungha; Kim, Tae-Su; Lee, Sung-Pyo; Park, Sung Kyeong

2014-01-01

96

Polysaccharide Isolated from Angelica sinensis Inhibits Hepcidin Expression in Rats with Iron Deficiency Anemia  

PubMed Central

Abstract A novel polysaccharide named Angelica sinensis polysaccharide (ASP) was obtained from the powdered and defatted roots of A. sinensis (Oliv.) Diels. The molecular weight of ASP was determined to be 78?kDa and was 95.0% sugars consisting of mostly arabinose, glucose, and galactose with a molar ratio of 1:5.68:3.91. A previous study indicated that ASP may increase plasma iron levels by suppressing the expression of hepcidin, a negative regulator of body iron metabolism, in the liver. The present study aims to clarify the inhibitory effect of ASP on hepcidin expression in rat models of iron deficiency anemia (IDA), and clarify the mechanisms involved. It was demonstrated that ASP significantly reduced hepcidin expression by inhibiting the expression of mothers against decapentaplegic protein 4 (SMAD4) in liver and stimulating the secretion of erythropoietin, which further downregulated hepcidin by repressing CCAAT/enhancer-binding protein ? (C/EBP?) and the phosphorylation of signal transducer and activator of transcription 3/5. The results indicate that ASP can suppress the expression of hepcidin in rats with IDA, and may be useful for the treatment of IDA.

Liu, Jin-Yu; Zhang, Yu; You, Ru-Xu; Zeng, Fang; Guo, Dan

2012-01-01

97

Chromatographic and mass spectrometric fingerprinting analyses of Angelica sinensis (Oliv.) Diels-derived dietary supplements.  

PubMed

Angelica sinensis (Oliv.) Diels ("Danggui" in Chinese) is one of the most commonly used traditional Chinese medicines. It has been used to invigorate blood circulation for the treatment of anemia, hypertension, chronic bronchitis, asthma, rheumatism, and cardiovascular diseases. There are a number of A. sinensis-derived dietary supplements in the US markets. However, no study have been conducted to investigate the quality of these dietary supplements. In this paper, high-performance liquid chromatographic and flow-injection mass spectrometric fingerprints were both evaluated to assess the consistency of A. sinensis-derived dietary supplements. Similarity analysis was carried out on the high-performance liquid chromatographic (HPLC) fingerprints. Meanwhile, principal component analysis (PCA) was performed on the data obtained from flow-injection mass spectrometric (FIMS) fingerprints, which can analyze each sample in 2 min, compared with 30 min required for the chromatographic fingerprint. Both methods show significant chemical differences between samples that may be due to differences in growing locations, growing conditions, harvesting times, and/or botanical processing. The loading plots obtained from PCA singled out the discriminatory ions that were responsible for chemical differences of A. sinensis-derived dietary supplements. PMID:23314619

Zhao, Yang; Sun, Jianghao; Yu, Liangli Lucy; Chen, Pei

2013-05-01

98

Extraction and chemical characterization of Angelica sinensis polysaccharides and its antioxidant activity.  

PubMed

In the present study, the Angelica sinensis polysaccharides (ASP) extraction procedure was optimized by an L9 (3(4)) orthogonal array experimental design (OAD) with four factors at three levels. Under the optimal extraction condition (extraction time 180 min, ratio of water to solid 6, extraction temperature 100°C, and extraction number 4), extraction yield of ASP was 5.6%. Rabbits were fed for 40 days with A. sinensis polysaccharides at a dose of 150 or 300 mg/kg body weight, respectively. At the end of 40 days, animals received cerebral ischemia reperfusion operation. CT perfusion imaging (CTP) analysis showed that rCBF and rCBV were significantly increased, whereas rMTT and rTTP were decreased in the ischemia cerebral tissue compared to CIR group rabbits. ASP significantly decreased oxidative damage, and increased antioxidant enzymes activities in brains of CIR animals. Moreover, ASP significantly enhanced the Ach, Na(+),K(+)-ATPase, Ca(2+),Mg2(+)-ATPase and glucose levels, decreased AChE activity in brain tissue of the experimental animals. These results suggest a potent role of ASP in protection of brain oxidative injury in CIR animals. PMID:23544627

Ai, Songtao; Fan, Xindong; Fan, Linfeng; Sun, Qi; Liu, Yu; Tao, Xiaofeng; Dai, Kerong

2013-05-15

99

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae).  

PubMed

Angelica sinensis is a highly valued medicinal herb, known as female ginseng that is widely cultivated in China. Although A. sinensis is in great demand due to its multiple medicinal and food applications, its early bolting rate (almost 40%) seriously affects crop quality. To better understand its flowering mechanism, cDNA-amplified RFLP analysis was employed to look for gene expression differences between flower bud and shoot apical meristem tissues. Sixty-four primer sets were used, with each primer set amplified to 60 transcript-derived fragments. Some transcript-derived fragments were expressed only in the flower bud. After cloning, sequencing and a homology search, 46 distinct sequences were obtained; 26 of these were found to have homologous sequences in databases. These included trans-caffeoyl-CoA 3-O-methyltransferase, 1-deoxy-d-xylulose 5-phosphate reductoisomerase, 15-cis-zeta-carotene isomerase, isoamylase, and calmodulin-binding protein. These genes are closely related to pollen germination and pollen tube growth, terpenoid backbone biosynthesis, and other metabolic pathways. Confirmation of differential expression of 10 sequences was obtained by semi-quantitative RT-PCR, showing higher expression levels in flower buds. PMID:22535385

Yu, G; Ma, Y X; Duan, J-A; Song, B S; He, Z Q

2012-01-01

100

Polysaccharide isolated from Angelica sinensis inhibits hepcidin expression in rats with iron deficiency anemia.  

PubMed

A novel polysaccharide named Angelica sinensis polysaccharide (ASP) was obtained from the powdered and defatted roots of A. sinensis (Oliv.) Diels. The molecular weight of ASP was determined to be 78?kDa and was 95.0% sugars consisting of mostly arabinose, glucose, and galactose with a molar ratio of 1:5.68:3.91. A previous study indicated that ASP may increase plasma iron levels by suppressing the expression of hepcidin, a negative regulator of body iron metabolism, in the liver. The present study aims to clarify the inhibitory effect of ASP on hepcidin expression in rat models of iron deficiency anemia (IDA), and clarify the mechanisms involved. It was demonstrated that ASP significantly reduced hepcidin expression by inhibiting the expression of mothers against decapentaplegic protein 4 (SMAD4) in liver and stimulating the secretion of erythropoietin, which further downregulated hepcidin by repressing CCAAT/enhancer-binding protein ? (C/EBP?) and the phosphorylation of signal transducer and activator of transcription 3/5. The results indicate that ASP can suppress the expression of hepcidin in rats with IDA, and may be useful for the treatment of IDA. PMID:22985399

Liu, Jin-Yu; Zhang, Yu; You, Ru-Xu; Zeng, Fang; Guo, Dan; Wang, Kai-Ping

2012-10-01

101

Volatile compounds from roots, stems and leaves of Angelica acutiloba growing in Taiwan.  

PubMed

The present study analyzed and compared the volatile compounds in fresh Angelica acutiloba roots, stems and leaves both qualitatively and quantitatively. The volatile compounds were isolated by either steam distillation (SD) or headspace-solid phase microextraction (HS-SPME). A total of 61 compounds were identified using gas chromatography/mass spectrometry (GC/MS). All 61 compounds were verified by SD, with 3n-butyl phthalide, gamma-terpinene, p-cymene and cis-beta-ocimene as the main compounds. Thirty-three compounds were verified by HS-SPME, with gamma-terpinene and p-cymene as the main compounds. The leaf samples contained the highest essential oil content. Compared with SD, HS-SPME sampling resulted in relatively higher amounts of highly volatile monoterpenes and lower amounts of less volatile compounds such as 3n-butyl phthalide. These findings demonstrate that A. acutiloba roots, stems and leaves have high 3n-butyl phthalide contents; thus, all parts of A. acutiloba may be used for further application and development. PMID:24868890

Chen, Hsin-Chun; Tsaia, Yi-; Linb, Li-Yun; Wu, Chin-Sheng; Tai, Shan-Pao; Chen, Yu-Chang; Chiang, Hsiu-Mei

2014-04-01

102

Antibacterial and anti-inflammatory activity of traditional Chinese herb pairs, Angelica sinensis and Sophora flavescens.  

PubMed

The purpose of the present study was to investigate the antibacterial and anti-inflammatory activity of Angelica sinensis extract (AE), Sophora flavescens extract (SE), and herb pair A. sinensis and S. flavescens extract (HPE). Endotoxin-induced uveitis (EIU) was induced in rats by a footpad injection of lipopolysaccharide. The anti-inflammatory potential of AE, SE, and HPE in the regulation of nuclear factor kappa B (NF-?B), maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1? (IL-1?), inducible nitric oxide synthase (iNOS) and tumor necrosis factor-? (TNF-?), adhesion molecule (ICAM-1), and cyclooxygenase-2 (COX-2) was determined by ELISA and immunohistochemistry. HPE showed strong antibacterial activity at all tested concentrations (1.25, 2.5, and 5 ?g/ml) to Escherichia coli, Staphylococcus aureus, and Shigella Castellani and Chalmers. HPE significantly inhibited EIU-induced upregulation of NF-?B activation and the production of IL-1?, TNF-?, iNOS, ICAM-1, and COX-2. Moreover, HPE suppressed MDA and infiltration of PMN. The study supports the hypothesis that the antipimple and anti-eczema activities of Dangguikushen compound recipe are attributed to herb pairs, A. sinensis and S. flavescens, used in combination. PMID:21976127

Han, Chunchao; Guo, Jianyou

2012-06-01

103

Chromatographic and mass spectrometric fingerprinting analyses of Angelica sinensis (Oliv.) Diels-derived dietary supplements  

PubMed Central

Angelica sinensis (Oliv.) Diels (“Danggui” in Chinese) is one of the most commonly used Traditional Chinese Medicines (TCMs). It has been used to invigorate blood circulation for the treatment of anemia, hypertension, chronic bronchitis, asthma, rheumatism and cardiovascular diseases. There are a number of A. sinensis-derived dietary supplements in the U. S. markets. However, no study has been conducted to investigate the quality of these dietary supplements. In this paper, high-performance liquid chromatographic and flow-injection mass spectrometric fingerprints were both evaluated to assess the consistency of A. sinensis-derived dietary supplements. Similarity analysis was carried out on the high-performance liquid chromatographic (HPLC) fingerprints. Meanwhile, principal component analysis (PCA) was performed on the data obtained from flow-injection mass spectrometric (FIMS) fingerprints, which cananalyze each sample in 2 min, compared to 30 min required for the chromatographic fingerprint. Principal component analysis (PCA) of the FIMS fingerprints was performed. Both methods show significant chemical differences between samples that may be due to differences in growing locations, growing conditions, harvesting times, and/or botanical processing. The loading plots obtained from PCA singled out the discriminatory ions that were responsible for chemical differences of A. sinensis-derived dietary supplements.

Zhao, Yang; Sun, Jianghao; Yu, Liangli (Lucy); Chen, Pei

2013-01-01

104

Effects of gamma irradiation on the yields of volatile extracts of Angelica gigas Nakai  

NASA Astrophysics Data System (ADS)

The study was carried out to determine the effects of gamma irradiation on the volatile flavor components including essential oils, of Angelica gigas Nakai. The volatile organic compounds from non- and irradiated A. gigas Nakai at doses of 1, 3, 5, 10 and 20 kGy were extracted by a simultaneous steam distillation and extraction (SDE) method and identified by GC/MS analysis. A total of 116 compounds were identified and quantified from non- and irradiated A. gigas Nakai. The major volatile compounds were identified 2,4,6-trimethyl heptane, ?-pinene, camphene, ?-limonene, ?-eudesmol, ?-murrolene and sphatulenol. Among these compounds, the amount of essential oils in non-irradiated sample were 77.13%, and the irradiated samples at doses of 1, 3, 5, 10 and 20 kGy were 84.98%, 83.70%, 83.94%, 82.84% and 82.58%, respectively. Oxygenated terpenes such as ?-eudesmol, ?-eudesmol, and verbenone were increased after irradiation but did not correlate with the irradiation dose. The yields of active substances such as essential oil were increased after irradiation; however, the yields of essential oils and the irradiation dose were not correlated. Thus, the profile of composition volatiles of A. gigas Nakai did not change with irradiation.

Seo, Hye-Young; Kim, Jun-Hyoung; Song, Hyun-Pa; Kim, Dong-Ho; Byun, Myung-Woo; Kwon, Joog-Ho; Kim, Kyong-Su

2007-11-01

105

Chemical composition and antioxidant activities of different polysaccharides from the roots of Angelica dahurica.  

PubMed

The total crude polysaccharides (CADPs), isolated from the roots of Angelica dahurica by H(2) O extraction, EtOH precipitation, and dialysis, and the four fractions ADP1, ADP2, ADP3, and ADP4, obtained by gel filtration of the CADPs, were analyzed to characterize their composition and evaluated for their antioxidant activity using different in vitro tests such as the malondialdehyde (MDA)-production, the ferrous ion (Fe(2+) )-chelating, and the HO(.) radical-scavenging assays. The predominant neutral monosaccharides in the four fractions were identified as arabinose, galactose, and glucose, while the composition and ratio of the monosaccharides were different between the fractions. The CADPs and its fractions were found to significantly inhibit lipid peroxidation, chelate Fe(2+) , and scavenge HO(.) radicals, indicating that these polysaccharides possessed antioxidant activity. Among the four fractions, ADP4 exhibited the strongest antioxidant activity, which was stronger than that of the control antioxidant vitamin E (Vit E). Taken together, the chemical composition of these polysaccharides might affect their antioxidant activity, and ADP4 could be explored as a source of potential novel natural antioxidants for food and pharmaceutical purposes. PMID:21674784

Xu, Shi-Fang; Ye, Yi-Ping; Li, Xiao-Yu; Chen, Feng-Yang

2011-06-01

106

In vitro GABA-transaminase inhibitory compounds from the root of Angelica dahurica.  

PubMed

The 80% aqueous MeOH extracts from the root of Angelica dahurica, found to inhibit the activities of GABA degradative enzymes GABA transaminase (GABA-T) and succinic semialdehyde dehydrogenase (SSADH), were fractionated using EtOAc, n-BuOH and H2O. Repeated column chromatography for the EtOAc and n-BuOH fractions led to the isolation of two new coumarins, oxypeucedanin hydrate-3''-butyl ether and isopraeroside IV along with six known coumarins, isoimperatorin, imperatorin, phellopterin, oxypeucedanin hydrate, nodakenin and 3'-hydroxymarmesinin, and two polyacetylenes, falcarindiol and octadeca-1,9-dien-4,6-diyn-3,8,18-triol. Of the isolated pure compounds, imperatorin and falcarindiol inactivated the GABA-T activities in both time- and concentration-dependent manners. The kinetic studies showed that imperatorin and falcarindiol reacted with the GABA-T with a second-order rate constant of 2.3 +/- 0.2 mm(-1) min(-1) and 1.5 +/- 0.1 mm(-1) min(-1), respectively. It is postulated that imperatorin and falcarindiol are able to elevate the neurotransmitter GABA levels in the central nervous system by an inhibitory action on the GABA degradative enzyme GABA-T. PMID:16261512

Choi, Soo Young; Ahn, Eun-Mi; Song, Myoung-Chong; Kim, Dae Won; Kang, Jeong Han; Kwon, Oh-Shin; Kang, Tae-Cheon; Baek, Nam-In

2005-10-01

107

Serotonergic Activity-Guided Phytochemical Investigation of the Roots of Angelica sinensis  

PubMed Central

Serotonin receptor (5-HT7) binding assay-directed fractionation of a methanol extract of the dried roots of Angelica sinensis (Apiaceae) led to the isolation and identification of twenty-one compounds including a new phenolic ester, angeliferulate (1), and three new phthalides, 10-angeloylbutylphthalide (2), sinaspirolide (3) and ansaspirolide (4), along with seventeen known compounds, p-hydroxyphenethyl trans-ferulate (5), Z-ligustilide (6), Z-butylidenephthalide (7), senkyunolide I (8), Z-6-hydroxy-7-methoxy-dihydroligustilide (9), N-butylbenzenesulphonamide (10), 11(S),16(R)-dihydroxy-octadeca-9Z,17-dien-12,14-diyn-1-yl acetate (11), (3R,8S)-falcarindiol (12), heptadeca-1-ene-9,10-epoxy-4,6-diyne-3,8-diol (13), oplopandiol (14), 8-hydroxy-1-methoxy-, Z-9-heptadecene-4,6-diyn-3-one (15), imperatorin, ferulic acid, vanillin, stigmasterol, sucrose, and 1,3-di-linolenin. This is the first report of a sulfonamide (10) identified from a higher plant source, although its presence needs further investigation. Biosynthetic pathways for dimeric phthalides 3 and 4 are proposed. Compound 5, 7, 11, 12, 15, and imperatorin exhibited affinity toward 5-HT7 receptors in a competitive binding assay.

Deng, Shixin; Chen, Shao-Nong; Yao, Ping; Nikolic, Dejan; van Breemen, Richard B.; Bolton, Judy L.; Fong, Harry H.S.; Farnsworth, Norman R.; Pauli, Guido F.

2006-01-01

108

[Effect of precursors on the production of coumarins in a suspension culture of Angelica archangelica L].  

PubMed

The effect of potential precursors (cinnamic acid, phenylalanine, tyrosine) in three concentrations (0.01; 0.1; 1 mmol/l) on the growth of the culture and coumarin production was investigated in the suspension culture of Angelica archangelica L. The cultures were cultivated under constant illumination (3500 lux) and in the dark. The growth of the culture in the dark was not affected by the precursors, cinnamic acid in a concentration of 1 mmol/l exerted toxic action. The growth of the culture cultivated under constant illumination was stimulated by tyrosine in a concentration of 0.1 and 1 mmol/l, and phenylalanine in a concentration of 0.1 mmol/l; phenylalanine and cinnamic acid in a concentration of 1 mmol/l inhibited the growth of the culture. In the cultivation in the dark, coumarin production was increased by phenylalanine in a concentration 0.01 mmol/l and by tyrosine in a concentration of 1 mmol/l. In the cultivation under constant illumination, coumarin production was decreased by the action of cinnamic acid, was not affected by tyrosine, and phenylalanine (0.01 and 0.1 mmol/l) increased it in comparison with the culture without precursors. PMID:11910743

Siatka, T; Kasparová, M

2002-01-01

109

Hepatoprotective effect of Angelica archangelica in chronically ethanol-treated mice.  

PubMed

Angelica archangelica (AAA) has been effectively used in folk medicines as a remedy against stomachal and intestinal disturbances, arthritic disease, etc. However, there is still lack of scientific proof about its antioxidant capability. This study aimed at investigating the effects of total AAA against chronic ethanol-induced hepatotoxicity. ICR mice were divided into five groups, each consisting of 10 animals. A single dose of ethanol (70%, 0.1 ml, p.o.) was used to induce hepatotoxicity in these mice which resulted in a significant elevation of the activities of serum GOT and GPT. Treatment of mice with AAA (10, 25, and 50 mg/kg p.o.) after 2 weeks ameliorated the ethanol-induced hepatotoxicity effects. Hepatotoxicity was evidenced by a significant increase in hepatic lipid peroxidation manifested as the presence of malondialdehyde. It was found that AAA inhibits the malondialdehyde formation in mouse liver homogenates both in vitro and in vivo. AAA is cytoprotective agent effective against chronic ethanol-induced hepatotoxicity, possibly through inhibition of the production of oxygen free radicals that cause lipid peroxidation, and hence indirectly protects the liver from oxidative stress. PMID:12711833

Yeh, Mei-Ling; Liu, Chi-Feng; Huang, Chun-Liang; Huang, Tian-Chyuan

2003-06-01

110

Correlation between antioxidant activities and phenolic contents of radix Angelicae sinensis (Danggui).  

PubMed

Radix Angelicae Sinensisis (RAS) is one of the most popular traditional Chinese herbal medicines. In the present study, six RAS extracts (i.e., phenolic extract PE, petroleum ether extract PEE, ethyl acetate extract EAE, absolute ethanol extract AEE, 95% ethanol extract 95 EE, and water extract WE) were prepared and their antioxidant activities measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical), ABTS [2,2'-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid diammonium salt)], Reducing power, *O(2-) and lipid peroxidation assays. In general, PE, PEE and EAE had relatively high antioxidant activity, followed by AEE with moderate activity, as compared with 95 EE and WE that had low activity. Their phenolic contents (including total phenolic, ferulic acid, caffeic acid, same as below) were then determined by HPLC or spectrophotometry. The sequence of phenolic contents was roughly identical with that of antioxidant activity. When the values of 1/IC(50) of various antioxidant assays were used to evaluate the level of antioxidant of the RAS extracts, (plot between 1/IC(50) values and phenolic contents), the correlation coefficient (R) ranged from 0.642 to 0.941, with an average value of 0.839. Significant positive correlations demonstrated that the antioxidant effects of RAS might generally be considered a result of the presence of the phenolic compounds, especially ferulic acid and caffeic acid. PMID:20032898

Li, Xican; Wu, Xiaoting; Huang, Ling

2009-01-01

111

Hydrodeoxygenation of the angelica lactone dimer, a cellulose-based feedstock: simple, high-yield synthesis of branched C7 -C10 gasoline-like hydrocarbons.  

PubMed

Dehydration of biomass-derived levulinic acid under solid acid catalysis and treatment of the resulting angelica lactone with catalytic K2 CO3 produces the angelica lactone dimer in excellent yield. This dimer serves as a novel feedstock for hydrodeoxygenation, which proceeds under relatively mild conditions with a combination of oxophilic metal and noble metal catalysts to yield branched C7 -C10 hydrocarbons in the gasoline volatility range. Considering that levulinic acid is available in >80?% conversion from raw biomass, a field-to-tank yield of drop-in, cellulosic gasoline of >60?% is possible. PMID:24474249

Mascal, Mark; Dutta, Saikat; Gandarias, Inaki

2014-02-10

112

A Systematic Review of the Mysterious Caterpillar Fungus Ophiocordyceps sinensis in Dong-ChongXiaCao (???? D?ng Ch?ng Xi? C?o) and Related Bioactive Ingredients  

PubMed Central

The caterpillar fungus Ophiocordyceps sinensis (syn.† Cordyceps sinensis), which was originally used in traditional Tibetan and Chinese medicine, is called either “yartsa gunbu” or “DongChongXiaCao (???? D?ng Chóng Xià C?o)” (“winter worm-summer grass”), respectively. The extremely high price of DongChongXiaCao, approximately USD $20,000 to 40,000 per kg, has led to it being regarded as “soft gold” in China. The multi-fungi hypothesis has been proposed for DongChongXiaCao; however, Hirsutella sinensis is the anamorph of O. sinensis. In Chinese, the meaning of “DongChongXiaCao” is different for O. sinensis, Cordyceps spp.,‡ and Cordyceps spƒ. Over 30 bioactivities, such as immunomodulatory, antitumor, anti-inflammatory, and antioxidant activities, have been reported for wild DongChongXiaCao and for the mycelia and culture supernatants of O. sinensis. These bioactivities derive from over 20 bioactive ingredients, mainly extracellular polysaccharides, intracellular polysaccharides, cordycepin, adenosine, mannitol, and sterols. Other bioactive components have been found as well, including two peptides (cordymin and myriocin), melanin, lovastatin, ?-aminobutyric acid, and cordysinins. Recently, the bioactivities of O. sinensis were described, and they include antiarteriosclerosis, antidepression, and antiosteoporosis activities, photoprotection, prevention and treatment of bowel injury, promotion of endurance capacity, and learning-memory improvement. H. sinensis has the ability to accelerate leukocyte recovery, stimulate lymphocyte proliferation, antidiabetes, and improve kidney injury. Starting January 1st, 2013, regulation will dictate that one fungus can only have one name, which will end the system of using separate names for anamorphs. The anamorph name “H. sinensis” has changed by the International Code of Nomenclature for algae, fungi, and plants to O. sinensis.

Lo, Hui-Chen; Hsieh, Chienyan; Lin, Fang-Yi; Hsu, Tai-Hao

2013-01-01

113

Mucilaginibacter herbaticus sp. nov., isolated from the rhizosphere of the medicinal plant Angelica sinensis.  

PubMed

A strictly aerobic, Gram-staining-negative, non-motile and rod-shaped bacterial strain, DR-9(T), was isolated from rhizosphere soil of the medicinal herb Angelica sinensis. Strain DR-9(T) grew at 20-40 °C, at pH 4.0-9.0 and in the presence of 0-1 % (w/v) NaCl. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1?7c and/or C16 : 1?6c and/or iso-C15 : 0 2-OH), MK-7 was the major isoprenoid quinone, and phosphatidylethanolamine and an unidentified aminophospholipid were the major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain DR-9(T) formed a lineage within the genus Mucilaginibacter and was closely related to Mucilaginibacter polysacchareus DRP28(T) (96.1 % sequence similarity), Mucilaginibacter myungsuensis HMD1056(T) (95.9 % sequence similarity), Mucilaginibacter ximonensis XM-003(T) (95.8 %) and Mucilaginibacter boryungensis BDR-9(T) (95.1 %). The status of strain DR-9(T) as a representative of a separate species was confirmed by DNA hybridization, with 38.6, 36.3 and 29.9 % DNA-DNA relatedness with M. polysacchareus DRP28(T), M. ximonensis XM-003(T) and M. boryungensis BDR-9(T), respectively. The genomic DNA G+C content of strain DR-9(T) was 49.8 %. These data suggest that strain DR-9(T) should be considered as a representative of a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter herbaticus sp. nov. is proposed. The type strain is DR-9(T) ( = KACC 16469(T) = NBRC 108839(T)). PMID:23291898

Lee, Hae-Ran; Han, Song-Ih; Rhee, Ki-Hyeong; Whang, Kyung-Sook

2013-08-01

114

Efficacy and Safety Assessment of T. Angelica Herbal Tonic, a Phytomedicinal Product Popularly Used in Nigeria  

PubMed Central

T. Angelica Herbal Tonic (TAHT) is a herbal product indicated for indigestion and constipation and highly patronized in Nigeria. In this study, the efficacy and safety of the herbal tonic in relation to the label claims were assessed. The effect on peristalsis in mice was evaluated by the charcoal meal model and in vitro using guinea pig ileum. The effects of TAHT on behavior, fertility, birth and organ weights were also determined. Teratogenic potential and reproductive toxicity were studied in pregnant rats. Acute toxicity studies showed that at doses above 5000?mg?kg?1, the herbal tonic did not cause lethality and produced no signs of intoxication in mice. The study did not show any gross behavioral changes in mice treated with 1000?mg?kg?1 of TAHT as compared with the negative control treatment. TAHT (400?mg?kg?1) exhibited a dose-dependent enhancement in the gastrointestinal tract motility in mice when compared with the negative control. At concentrations up to 300??g?mL?1, TAHT did not cause any significant effect on acetylcholine, histamine and nicotine-evoked contractions of guinea pig ileum preparation. It took an average of 31.25 ± 4.52 days for the TAHT-treated animals to litter, which is significantly (P < .05) different from the 55 ± 4.51 days recorded for the control treatment group. TAHT exhibited a modest fertility-promoting effect and showed lack of abortifacient and teratogenic properties in the study. Generally, the results of this study showed some favorable pharmacological effects of TAHT in animals which may authenticate some of the label claims.

Esimone, Charles O.; Akah, Peter A.; Nworu, Chukwuemeka S.

2011-01-01

115

Multi-component analysis in sun-dried and sulfur-fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination  

PubMed Central

Background: A new method has been developed for the simultaneous determination of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix before and after sulfur-fumigation using quantitative analysis of multi-components by a single marker (QAMS). Materials and Methods: The feasibility and accuracy of QAMS were checked by the external standard method, and various high-performance liquid chromatographic instruments and chromatographic conditions were investigated to verify its applicability. Using ferulic acid as the internal reference substance, and the contents of senkyunolide A and Z-ligustilide were calculated according to relative correction factors by high-performance liquid chromatography. Meanwhile, the influence of sulfur-fumigation on these chemical components in Angelicae Sinensis Radix were evaluated and discriminated by chromatographic fingerprint and chemometrics. Results: There was no significant difference observed between the QAMS method and the external standard method. Furthermore, sulfur-fumigation reduced the contents of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix by some degree, and the sun-drying and sulfur-fumigation processing could be easily discriminated by chromatographic fingerprint and chemometrics. Conclusion: QAMS is a convenient and accurate approach to analyzing multi-component when reference substances are unavailable, simultaneously, chemometrics is an effective way to discriminate sun-dried and sulfur-fumigated Angelicae Sinensis Radix.

Lou, Yajing; Cai, Hao; Liu, Xiao; Cao, Gang; Tu, Sicong; Li, Songlin; Ma, Xiaoqing; Qin, Kunming; Cai, Baochang

2014-01-01

116

A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach.  

PubMed

Angelica sinensis (AS) is a traditional Chinese herbal medicine that has been formulated clinically to treat various form of skin trauma and to help wound healing. However, the mechanism by which it works remains a mystery. In this study we have established a new platform to evaluate the pharmacological effects of total AS herbal extracts as well as its major active component, ferulic acid (FA), using proteomic and biochemical analysis. Cytotoxic and proliferation-promoting concentrations of AS ethanol extracts (AS extract) and FA were tested, and then the cell extracts were subject to 2D PAGE analysis. We found 51 differentially expressed protein spots, and these were identified by mass spectrometry. Furthermore, biomolecular assays, involving collagen secretion, migration, and ROS measurements, gave results that are consistent with the proteomic analysis. In this work, we have demonstrated a whole range of pharmacological effects associated with Angelica sinensis that might be beneficial when developing a wound healing pharmaceutical formulation for the herbal medicine. PMID:22536285

Hsiao, Chia-Yen; Hung, Ching-Yi; Tsai, Tung-Hu; Chak, Kin-Fu

2012-01-01

117

An Aqueous Extract of Radix Astragali, Angelica sinensis, and Panax notoginseng Is Effective in Preventing Diabetic Retinopathy.  

PubMed

Diabetic retinopathy (DR), in which inflammation has been implicated playing important roles, is one of the most common diabetes complications. Dang Gui Bu Xue Tang (DBT), an aqueous extract of Radix Astragali and Radix Angelica sinensis, is a classical prescription in Traditional Chinese Medicine for treating inflammation and ischemic diseases. Here, we investigated the effects of a modified recipe of DBT, with addition of Panax notoginseng, in treating diabetic retinopathy. An aqueous extract of Radix Astragali, Radix Angelica sinensis, and Panax notoginseng (RRP) was given to Goto-Kakizaki (GK) rats and streptozotocin-induced Sprague-Dawley (SD) rats. Leukostasis, vascular leakage, and acellular capillaries in retinal vasculature of animals were determined. Expression of retinal inflammatory biomarkers was assessed. We found that RRP reduced leukostasis, acellular capillaries, and vascular leakage compared to diabetic control rats. We also found that RRP decreased the expression of inflammatory factors including IL-1 ? , IL-6, TNF- ? , NF- ? B, MCP-1, ICAM-1, or VCAM-1 in the retinas of GK rats and reversed high glucose-induced inhibition of endothelial cell migration and proliferation in vitro. We conclude that RRP has a potent effect in preventing the pathogenesis and/or progression of DR and thus may serve as a promising nontoxic therapeutic approach of DR. PMID:23662142

Gao, Dehong; Guo, Yijuan; Li, Xuejun; Li, Xiumin; Li, Zhipeng; Xue, Mei; Ou, Zhimin; Liu, Ming; Yang, Mingxing; Liu, Suhuan; Yang, Shuyu

2013-01-01

118

[Fast determination of active components in Angelica dahurica extract using capillary electrochromatography with methacrylate ester-based monolithic columns].  

PubMed

The separation and determination of four important active components (imperatorin, isoimperatorin, phelloptorin and falcarindiol) from Angelica dahurica extract has been performed using capillary electrochromatography (CEC) with a methacrylate ester-based monolithic column. The effect of the porogen ratio on the column preparation was studied. The mobile phase composition, such as the concentration of organic solvent, the ionic strength and the pH of the buffer were also optimized. Under the optimized conditions (50% acetonitrile and 50% of a 20 mmol/L sodium dihydrogen phosphate electrolyte at pH 4.95, - 25 kV), a fast and baseline separation of the four analytes was achieved. The calibration curves showed a good linearity (r2 > 0.997) and the limits of detection were lower than 0.34 mg/L. The mean recoveries of the studied components ranged between 95.18% and 98.44%. The method developed is sensitive, reliable and suitable for the quality control. With this CEC system, the quality of Angelica dahurica extracts from 18 various regions was evaluated. PMID:20549974

Wang, Jiajing; Chen, Zhao; Wu, Yutian; Fan, Guorong

2010-03-01

119

Chinese Medicine Formula "Jian-Pi-Zhi-Dong Decoction" Attenuates Tourette Syndrome via Downregulating the Expression of Dopamine Transporter in Mice.  

PubMed

Jian-Pi-Zhi-Dong Decoction (JPZDD) is dedicated to the treatment for Tourette syndrome (TS) with the guidance of the theories of Traditional Chinese Medicine (TCM). This study aims to investigate the expression of dopamine transporter (DAT) in the striatum and stereotyped behavior of TS mice model by intervention of JPZDD. Mice were induced by 3,3'-iminodipropionitrile (IDPN, 350?mg kg(-1) day(-1), i.p.) for 7 days and divided into 4 groups (n = 20, each): control and IDPN groups were gavaged with saline and the remaining 2 groups with Tiapride (Tia, 50?mg kg(-1) day(-1)) and JPZDD (20?g kg(-1) day(-1)), respectively. The results showed that the scores of stereotyped behavior in IDPN+JPZDD group were significantly reduced. A noticeably increased (11)C-?-CFT binding at bilateral striatum was observed after administration of JPZDD versus that of IDPN or Tia. Immunohistochemistry and in situ hybridization studies manifested higher levels of DAT protein and mRNA in IDPN+JPZDD group. These findings not only demonstrated that JPZDD could effectively inhibit the abnormal behaviors of TS mice model, but also increase the level of DAT in striatum. Therefore, JPZDD could be one of potential treatments of patients with TS. PMID:23431337

Wang, Dao-Han; Li, Wei; Liu, Xiao-Fang; Zhang, Jin-Ming; Wang, Su-Mei

2013-01-01

120

Furocoumarins from Angelica dahurica with hepatoprotective activity on tacrine-induced cytotoxicity in Hep G2 cells.  

PubMed

Fractionation of the MeOH extract of Angelica dahurica Benth et Hook resulted in the isolation of six furocoumarins, imperatorin (1), isoimperatorin (2), (+/-)-byakangelicol (3), (+)-oxypeucedanin (4), (+)-byakangelicin (5), and (+)-aviprin (6). Among these, compounds 1 and 5 exhibited strong hepatoprotective activities, displaying EC(50) values of 36.6 +/- 0.98 and 47.9 +/- 4.6 microM, respectively. Compounds 3 and 4 showed moderate activities with EC(50) values of 112.7 +/- 5.35 and 286.7 +/- 6.36 microM, respectively. Silybin as a positive control showed the EC(50) value with 69.0 +/- 3.4 microM. Comparison of hepatoprotective activities for six furocoumarins 1 - 6 suggested that oxy-substitution at the C-9 position increased the hepatoprotective activity. PMID:12058329

Oh, Hyuncheol; Lee, Ho-Sub; Kim, Taewan; Chai, Kyu-Yun; Chung, Hun-Taeg; Kwon, Tae-Oh; Jun, Jung-Yang; Jeong, Ok-Sam; Kim, Youn-Chul; Yun, Young-Gab

2002-05-01

121

Imperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 Cells.  

PubMed

Imperatorin, a biologically active furanocoumarin from the roots of Angelica dahurica (Umbelliferae), was found to induce apoptosis in human promyelocytic leukaemia, HL-60 cells. DNA fragmentation assay, morphology-based evaluation, and flow cytometric analysis demonstrated that imperatorin at micromolar concentrations was able to trigger apoptosis of HL-60 cells. Neither necrosis nor differentiation was observed at cytotoxic micromolar concentrations of imperatorin. Further studies showed that the cytochrome c/caspase-9 pathway was responsible for imperatorin-induced apoptosis; i.e., mitochondrial membrane was depolarized, Bcl-2 was down-regulated, cytochrome c was released from mitochondria, caspase-9 and caspase-3 were activated, and poly(ADP-ribose) polymerase was cleaved. Furthermore, imperatorin-induced apoptosis was significantly blocked by Z-VAD-FMK (a broad spectrum caspase inhibitor), Z-LEHD-FMK (a caspase-9 inhibitor) and Ac-DMQD-CHO (a caspase-3 inhibitor), but not by Z-IEDT-FMK (a caspase-8 inhibitor). PMID:12193260

Pae, Hyun-Ock; Oh, Hyuncheol; Yun, Young-Gab; Oh, Gi-Su; Jang, Seon Il; Hwang, Kee-Myoung; Kwon, Tae-Oh; Lee, Ho-Sub; Chung, Hun-Taeg

2002-07-01

122

Identification and determination of active components in Angelica dahurica Benth and its medicinal preparation by capillary electrophoresis.  

PubMed

A micellar electrokinetic capillary chromatography (MECC) method for the separation of three active components, imperatorin (IM), isoimperatorin (IO) and scopoletin (SC), in Angelica dahurica Benth and its preparation was developed. The optimum separation for these analytes was achieved using 10 mM borate, 20 mM SDS and 10%(v/v) acetonitrile, with applied voltage of 22 kV. All experiments were performed using a 50.0 cm (42.5 cm effective length)x75 mum I.D. fused-silica capillary. The linear calibration range was 12.0-800.0 mug ml(-1) for IM, 10.0-850.0 mug ml(-1) for IO and 3.5-600.0 mug ml(-1) for SC. The recoveries of three constituents were from 91.3 to 108.7%. Also, the dissociation constant (pKa) of SC was determined by the CE method established in this paper. PMID:18968298

Ketai, W; Huitao, L; Xingguo, C; Yunkun, Z; Zhide, H

2001-05-30

123

Identification and characterization of a new virus in the genus Potyvirus from wild populations of Angelica lucida L. and A. genuflexa Nutt., family Apiaceae.  

PubMed

A novel potyvirus was discovered in Angelica lucida L. (wild celery) and A. genuflexa Nutt. (kneeling Angelica) (family Apiaceae) in the Matanuska- Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae (Chenopodium amaranticolor Coste et Reyn and Chenopodium quinoa Willd.), Solanaceae (Nicotiana benthamiana Domin, and N. clevlandii Gray.), and Apiaceae (Anethum graveolens L., Apium graveolens L. var. dulce (Miller)), Daucus carota L. subspecies sativus (Hoffm.) Arcang., and Petroselinum crispum (Miller) Nyman ex A. W. Hilland). The virus contained flexuous rods with an ssRNA genome approximately 9.5-10 k nts and a CP ( approximately 35 kDa) that reacted to a universal potyvirus monoclonal antibody in Western blot analysis. The sequenced genomic 3'-end ( approximately 1850 nt) contained a potyvirus genomic arrangement that included the 3'-terminus of the NIb (nuclear inclusion) gene, the CP (coat protein) gene, and a 3'-UTR (untranslated region) attached to a poly(A)tail. The CP amino acids had between 54 and 70% identity with 12 selected members from the genus Potyvirus. Phylogenetically, the Alaskan potyvirus clustered with three other apiaceous potyviruses from Australia. The novel Alaskan virus was confined to A. lucida L. and A. genuflexa Nutt. in nature, and was classified in the genus Potyvirus, family Potyviridae, and in part named after its natural plant hosts, angelica virus Y (AnVY). PMID:17558540

Robertson, N L

2007-01-01

124

Characterization of a cDNA coding for an extracellular calmodulin-binding protein from suspension-cultured cells of Angelica dahurica.  

PubMed

In order to characterize a specific extracellular 21-kDa calmodulin-binding protein (named: ECBP21) from Angelica dahurica L. suspension-cultured cells, the cDNA coding for the protein has been cloned. Here, Southern blot analysis shows that there are at least two copies of ECBP21 gene in Angelica genome. Using truncated versions of ECBP21 and synthetic peptide in CaM binding assays, we mapped the calmodulin-binding domain to a 16-amino acid stretch (residues 200-215) at the C-terminal region. The ECBP21 was localized in the cell wall area by the immunogold electron microscopy and by GFP labeling method. These results define ECBP21 as a kind of an extracellular calmodulin-binding protein (CaMBP). Furthermore, using Northern blot analysis, we examined the expression dynamics of ecbp21 during the incubation of Angelica suspension-cultured cells and the treatments with some growth regulators. The above studies further provide the molecular evidence for the existence of the gene coding for extracellular CaMBPs and imply a possible role for ECBP21. PMID:15891898

Mao, Guo-Hong; Hou, Li-Xia; Ding, Cun-Bao; Cui, Su-Juan; Sun, Da-Ye

2005-10-01

125

Altered expression of genes profiles modulated by a combination of Astragali Radix and Angelicae Sinensis Radix in obstructed rat kidney.  

PubMed

The decoction of a combination of two Chinese herbs, Astragali Radix (the roots of Astragalus membranaceus var. mongholicus) and Angelicae Sinensis Radix (the roots of Angelica sinensis), here named as A&A, has been demonstrated to have renoprotective effects in several animal models and may be considered as a complementary therapeutic medicine for chronic kidney disease. In this study, genomic approaches were employed to identify expression signatures in the obstructed kidney, which may be linked to the molecular actions associated with anti-fibrotic effects of A&A. Ninety-six male Wistar rats were divided randomly into sham, SAA (sham + A&A), UUO (unilateral ureteral obstruction), and UAA (UUO + A&A) groups. The rats in the SAA and UAA groups were administered A&A (14?g/kg) by oral gavage once daily; the ones in the sham and UUO groups were given equal volumes of water. Eight rats from each group were sacrificed at days 3, 7, and 10 after the operation, respectively. Changes in gene expression in the kidneys were determined using Affymetrix RAE-230A GeneChips. The differential expression of known genes between UAA and UUO was confirmed by RT-PCR. The results revealed that 40, 65, and 104 genes were upregulated and 30, 36, and 40 genes downregulated in UUO compared with the sham group at days 3, 7, and 10, respectively. Compared to the UUO group, eight genes were upregulated and two genes were downregulated at day 3 in the UAA group, and two genes were upregulated at day 10. These genes included transient receptor protein 3 (TRP3), bone marrow stromal cell antigen 1 (BST-1), peroxisomal biogenesis factor 6 (PEX6), xanthine dehydrogenase (XDH), cytochrome P450 subfamily I member A1 (CYP1A1), serine/cysteine proteinase inhibitor clade E member1 (PAI-1), fibroblast growth factor 23 (FGF23), and five ESTs. Among these genes, differential expression of PAI-1, FGF23, and CYP1A1 were further confirmed by RT-PCR. These data provide the evidence that the anti-fibrotic effects of A&A are mediated through multiple pathways in obstructive nephropathy, and novel mechanisms may be involved in the increasing degeneration of ECM, decreasing ROS reaction, and regulation of the calcium-phosphate metabolism. PMID:20195958

Meng, Liqiang; Van Putten, Vicki; Qu, Lei; Nemenoff, Raphael A; Shang, Ming-Ying; Cai, Shao-Qing; Li, Xiaomei

2010-09-01

126

Angelica archengelica extract induced perturbation of rat skin and tight junctional protein (ZO-1) of HaCaT cells  

PubMed Central

Background and purpose of the study Herbal enhancers compared to the synthetic ones have shown less toxis effects. Coumarins have been shown at concentrations inhibiting phospoliphase C-Y (Phc-Y) are able to enhance tight junction (TJ) permeability due to hyperpoalation of Zonolous Occludense-1 (ZO-1) proteins. The purpose of this study was to evaluate the influence of ethanolic extract of Angelica archengelica (AA-E) which contain coumarin on permeation of repaglinide across rat epidermis and on the tight junction plaque protein ZO-1 in HaCaT cells. Methods Transepidermal water loss (TEWL) from the rat skin treated with different concentrations of AA-E was assessed by Tewameter. Scanning and Transmission Electron Microscopy (TEM) on were performed on AA-E treated rat skin portions. The possibility of AA-E influence on the architecture of tight junctions by adverse effect on the cytoplasmic ZO-1 in HaCaT cells was investigated. Finally, the systemic delivery of repaglinide from the optimized transdermal formulation was investigated in rats. Results The permeation of repaglinide across excised rat epidermis was 7-fold higher in the presence of AA-E (5% w/v) as compared to propylene glycol:ethanol (7:3) mixture. The extract was found to perturb the lipid microconstituents in both excised and viable rat skin, although, the effect was less intense in the later. The enhanced permeation of repaglinide across rat epidermis excised after treatment with AA-E (5% w/v) for different periods was in concordance with the high TEWL values of similarly treated viable rat skin. Further, the observed increase in intercellular space, disordering of lipid structure and corneocyte detachment indicated considerable effect on the ultrastructure of rat epidermis. Treatment of HaCaT cell line with AA-E (0.16% w/v) for 6 hrs influenced ZO-1 as evidenced by reduced immunofluorescence of anti-TJP1 (ZO-1) antibody in Confocal Laser Scanning Microscopy studies (CLSM) studies. The plasma concentration of repaglinide from transdermal formulation was maintained higher and for longer time as compared to oral administration of repaglinide. Major conclusion Results suggest the overwhelming influence of Angelica archengelica in enhancing the percutaneous permeation of repaglinide to be mediated through perturbation of skin lipids and tight junction protein (ZO-1).

Kaushal, N.; Naz, S.; Tiwary, AK.

2011-01-01

127

[Effect of combined administration of Angelica polysaccharide and cytarabine on liver of human leukemia NOD/SCID mouse model].  

PubMed

Leukemia is a type of malignant tumors of hematopoietic system with the abnormal increased immature leukemia cells showing metastasis and invasion ability. Liver is one of the main targets of the leukemia cells spread to, where they may continue to proliferate and differentiate and cause liver function damage, even liver failure. Our previous studies showed that Angelica polysscharides (APS), the main effective components in Angelica sinensis of Chinese traditional medicine, was able to inhibit the proliferation and induced differentiation of the leukemia cells, however, its effect on the liver during the treatment remains elucidated. In the present study, the human leukemia NOD/SCID mouse model were established by implantation human leukemia K562 cells line, then the leukemia mouse were treated with APS, Ara-c or APS + Ara-c respectively by peritoneal injection for 14 days, to explore the effect and mechanism of the chemicals on the mouse liver. Compared to the human leukemia NOD/SCID mouse model group with the treatments of APS, Ara-c and APS + Ara-c, We found that severe liver damage and pathological changes of the liver were able to alleviate: First, the number of white blood cells in the peripheral blood was significantly lower and with less transplanted K562 leukemia cells; Second, liver function damage was alleviated as liver function tests showed that alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBiL) were significantly reduced, while the albumin (Alb) was notably increased; Third, liver antioxidant ability was improved as the activities of the antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were significantly increased, and the contents of GSH and malonaldehyde (MDA) were decreased significantly in the liver; Fourth, the inflammation of the liver was relieved as the level of IL-1beta and IL-6, the inflammatory cytokines, were decreased significantly in the liver. Fifth, liver index was increased as the pathological observation showed that leukemia cells with diffused infiltration into the liver lobules were significantly reduced and with a remarkable increase of apoptotic positive cell rate by TUNEL test. Furthermore, the APS + Ara-c combined administration showed an even more significant positive effect. In conclusion, the APS, Ara-c therapy reduced the accumulation of leukemia cells within the liver, reduced the liver function damage and levels of inflammatory factors, improved antioxidant capacity of the liver tissue and thus alleviate the pathological changes of the liver. Moreover, the APS + Ara-c combination therapy may have an additive effect. PMID:24754180

Zhu, Jia-Hong; Xu, Chun-Yan; Mu, Xin-Yi; Liu, Jun; Zhang, Meng-Si; Jia, Dao-Yong; Zhang, Yan-Yan; Huang, Guo-Ning; Wang, Ya-Ping

2014-01-01

128

Effect of fermented Angelicae gigantis Radix on carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats.  

PubMed

This study is aimed to evaluate the protective effect of fermented Angelicae gigantis Radix (AGR) with Monascus purpureus strain on carbon tetrachloride (CCl(4))-induced hepatotoxicity and oxidative stress in rats. The activities of liver marker enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and the levels of lipid peroxidation were increased when CCl(4) was treated but these parameters were significantly decreased by fermented AGR treatment. CCl(4) treatment exhibited decrease in serum concentrations of triglyceride, total cholesterol, HDL-cholesterol, and free fatty acids, and these were also decreased by fermented AGR administration. The level of serum leptin was significantly lower in fermented AGR administration than that in normal control group. CCl(4) treatment significantly increased the concentration of liver triglyceride. The current study observed significant elevations of the thiobarbituric acid-reactive substances (TBARS) levels in the liver homogenate, mitochondrial, and microsomal fractions of CCl(4) control group compared with normal control group. CCl(4) treatment resulted in a significant decrease in the levels of plasma and hepatic glutathione, but these reductions were significantly increased by fermented AGR administration. CCl(4) induced the marked hepatocytes necrosis and fatty accumulation around the central veins. Accordingly, fermented AGR may be an ideal candidate for the hepatoprotective effect in animal model. PMID:21854183

Cha, Jae-Young; Ahn, Hee Young; Moon, Hyung-In; Jeong, Yong-Kee; Cho, Young-Su

2012-04-01

129

Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis  

PubMed Central

This study investigated the effect of Angelica sinensis polysaccharides (APS-3c) on rat osteoarthritis (OA) model in vivo and rat interleukin-1-beta- (IL-1?-) stimulated chondrocytes in vitro. APS-3c was administrated into rat OA knee joints and had protective effects on rat OA cartilage in vivo. Primary rat articular chondrocytes were cotreated with APS-3c and IL-1???in vitro. 2~50??g/mL APS-3c had no effect on chondrocytes viability, whereas it increased the proteoglycans (PGs) synthesis inhibited by IL-1?. Microarray analysis showed that the significant changes were concentrated in the genes which were involved in PGs synthesis. RT-PCR confirmed that treatment with APS-3c increased the mRNA expression of aggrecan and glycosyltransferases (GTs) inhibited by IL-1? but did not affect the mRNA expression of matrix-degrading enzymes. These results indicate that APS-3c can improve PGs synthesis of chondrocytes on rat OA model in vivo and IL-1?-stimulated chondrocytes in vitro, which is due to the promotion of the expression of aggrecan and GTs involved in PGs synthesis but not the inhibition of the expression of matrix-degrading enzymes. Our findings suggest the clinical relevance of APS-3c in the prospective of future alternative medical treatment for OA.

Qin, Jun; Liu, Yan-song; Liu, Jun; Li, Jing; Tan, Yang; Li, Xiao-jun; Magdalou, Jacques; Mei, Qi-bing; Wang, Hui; Chen, Liao-bin

2013-01-01

130

The Methanol Extract of Angelica sinensis Induces Cell Apoptosis and Suppresses Tumor Growth in Human Malignant Brain Tumors.  

PubMed

Glioblastoma multiforme (GBM) is a highly vascularized and invasive neoplasm. The methanol extract of Angelica sinensis (AS-M) is commonly used in traditional Chinese medicine to treat several diseases, such as gastric mucosal damage, hepatic injury, menopausal symptoms, and chronic glomerulonephritis. AS-M also displays potency in suppressing the growth of malignant brain tumor cells. The growth suppression of malignant brain tumor cells by AS-M results from cell cycle arrest and apoptosis. AS-M upregulates expression of cyclin kinase inhibitors, including p16, to decrease the phosphorylation of Rb proteins, resulting in arrest at the G0-G1 phase. The expression of the p53 protein is increased by AS-M and correlates with activation of apoptosis-associated proteins. Therefore, the apoptosis of cancer cells induced by AS-M may be triggered through the p53 pathway. In in vivo studies, AS-M not only suppresses the growth of human malignant brain tumors but also significantly prolongs patient survival. In addition, AS-M has potent anticancer effects involving cell cycle arrest, apoptosis, and antiangiogenesis. The in vitro and in vivo anticancer effects of AS-M indicate that this extract warrants further investigation and potential development as a new antibrain tumor agent, providing new hope for the chemotherapy of malignant brain cancer. PMID:24319475

Lin, Yu-Ling; Lai, Wen-Lin; Harn, Horng-Jyh; Hung, Pei-Hsiu; Hsieh, Ming-Chang; Chang, Kai-Fu; Huang, Xiao-Fan; Liao, Kuang-Wen; Lee, Ming-Shih; Tsai, Nu-Man

2013-01-01

131

Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis.  

PubMed

This study investigated the effect of Angelica sinensis polysaccharides (APS-3c) on rat osteoarthritis (OA) model in vivo and rat interleukin-1-beta- (IL-1 ? -) stimulated chondrocytes in vitro. APS-3c was administrated into rat OA knee joints and had protective effects on rat OA cartilage in vivo. Primary rat articular chondrocytes were cotreated with APS-3c and IL-1 ? ??in vitro. 2~50? ? g/mL APS-3c had no effect on chondrocytes viability, whereas it increased the proteoglycans (PGs) synthesis inhibited by IL-1 ? . Microarray analysis showed that the significant changes were concentrated in the genes which were involved in PGs synthesis. RT-PCR confirmed that treatment with APS-3c increased the mRNA expression of aggrecan and glycosyltransferases (GTs) inhibited by IL-1 ? but did not affect the mRNA expression of matrix-degrading enzymes. These results indicate that APS-3c can improve PGs synthesis of chondrocytes on rat OA model in vivo and IL-1 ? -stimulated chondrocytes in vitro, which is due to the promotion of the expression of aggrecan and GTs involved in PGs synthesis but not the inhibition of the expression of matrix-degrading enzymes. Our findings suggest the clinical relevance of APS-3c in the prospective of future alternative medical treatment for OA. PMID:23861713

Qin, Jun; Liu, Yan-Song; Liu, Jun; Li, Jing; Tan, Yang; Li, Xiao-Jun; Magdalou, Jacques; Mei, Qi-Bing; Wang, Hui; Chen, Liao-Bin

2013-01-01

132

Structural characterization and radioprotection of bone marrow hematopoiesis of two novel polysaccharides from the root of Angelica sinensis (Oliv.) Diels.  

PubMed

Two novel homogeneous polysaccharides, APS-1a and APS-3a were successfully isolated from the root of Angelica sinensis. APS-1a was composed of galactose, arabinose and glucose in a relatively molar percentage of 57.34%, 27.67% and 14.98%, and had a molecular weight of 49.0kDa, whereas APS-3a was composed of galactose, arabinose and glucose in a relatively molar percentage of 84.54%, 6.50%, and 8.96%, and had a molecular weight of 65.4kDa. APS-1a and APS-3a mainly consisted of 1,4-linked galactose, 1,3,6-linked galactose, T-galactose and T-arabinose, and the molar ratio of each linkage was different between APS-1a and APS-3a. The bioactivity analysis showed that APS-1a and APS-3a increased the thymus and spleen index, the number of red blood cell (RBC) and white blood cell (WBC) in peripheral blood and the cellularity of bone marrow cell numbers in irradiated mice, protected mice against radiation-induced micronucleus formation in bone marrow, suggesting that polysaccharides could be used as radioprotective agents, especially for promoting bone marrow hematopoiesis. PMID:23063893

Zhao, Lu; Wang, Yin; Shen, Han-lin; Shen, Xu-dong; Nie, Yan; Wang, Ying; Han, Ting; Yin, Ming; Zhang, Qiao-yan

2012-12-01

133

Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis.  

PubMed

Three exopolysaccharide-producing bacteria, designated strains DRP28(T), DRP29 and DRP31, were isolated from the rhizoplane of Angelica sinensis from the Geumsan, Republic of Korea. Cells were straight rods, Gram reaction-negative, aerobic, non-motile, and catalase- and oxidase- positive. Flexirubin-type pigments were absent. Phylogenetic analysis of the 16S rRNA gene indicated that these bacteria belong to the genus Mucilaginibacter in the phylum Bacteroidetes. 16S rRNA gene sequence similarities to strains of recognized species of the genus Mucilaginibacter were 93.8-97.4%. The major fatty acids were iso-C(15:0) and summed feature 3 (C(16:1)?7c and/or iso-C(15:0) 2-OH). The strains contained MK-7 as the major isoprenoid quinone. Strains DRP28(T), DRP29 and DRP31 formed a single, distinct genomospecies with DNA G+C contents of 41.9-42.7 mol% and DNA hybridization values of 82.6-86.8%; the strains exhibited DNA-DNA hybridization values of only 20.4-41.3% with related species of the genus Mucilaginibacter. On the basis of evidence presented in this study, strains DRP28(T), DRP29 and DRP31 were considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter polysacchareus sp. nov. is proposed. The type strain is DRP28(T) (=KACC 15075(T) =NBRC 107757(T)). PMID:21602363

Han, Song-Ih; Lee, Hyo-Jin; Lee, Hae-Ran; Kim, Ki-Kwang; Whang, Kyung-Sook

2012-03-01

134

Ferulic Acid, an Angelica sinensis-Derived Polyphenol, Slows the Progression of Membranous Nephropathy in a Mouse Model.  

PubMed

Membranous nephropathy (MN) is a leading cause of adult nephrotic syndrome but lacks adequate treatment. Different extracts of Angelica sinensis (AS) and one of its active compounds, ferulic acid (FA), were used to evaluate the therapeutic effects in a MN mouse model. The MN model was grouped into three subgroups: no treatment (N-T), treatment at induction of MN (Pre-T), and treatment after full-blown MN (Post-T). The results showed that the methanol (ME) layer of AS extract exhibited a therapeutic effect on MN-induced proteinuria. The ME layer-enriched compound, FA, improved the hypoalbuminemia, hyperlipidemia, and proteinuria in both Pre-T and Post-T groups. Ferulic acid also reduced the formation of oxidative protein products and increased the synthesis of antioxidant enzymes in groups Pre-T and Post-T. Regarding angiogenesis factors, the antiangiogenic factors in renal glomeruli were increased in group N-T, but, after FA treatment, only one of the antiangiogenic factors, thrombospondin-1, showed a significant decrease. Furthermore, the expression of Th2 predominant showed significant decrease in both Pre-T and Post-T groups when compared to that of N-T group. In summary, FA retarded the progression of MN, and the mechanisms involved the regulation of oxidative stresses, angiogenic and antiangiogenic factors, and attenuation of Th2 response. PMID:22844329

Cheng, Chao-Wen; Chang, Wen-Liang; Chang, Li-Cheng; Wu, Chia-Chao; Lin, Yuh-Feng; Chen, Jin-Shuen

2012-01-01

135

The Methanol Extract of Angelica sinensis Induces Cell Apoptosis and Suppresses Tumor Growth in Human Malignant Brain Tumors  

PubMed Central

Glioblastoma multiforme (GBM) is a highly vascularized and invasive neoplasm. The methanol extract of Angelica sinensis (AS-M) is commonly used in traditional Chinese medicine to treat several diseases, such as gastric mucosal damage, hepatic injury, menopausal symptoms, and chronic glomerulonephritis. AS-M also displays potency in suppressing the growth of malignant brain tumor cells. The growth suppression of malignant brain tumor cells by AS-M results from cell cycle arrest and apoptosis. AS-M upregulates expression of cyclin kinase inhibitors, including p16, to decrease the phosphorylation of Rb proteins, resulting in arrest at the G0-G1 phase. The expression of the p53 protein is increased by AS-M and correlates with activation of apoptosis-associated proteins. Therefore, the apoptosis of cancer cells induced by AS-M may be triggered through the p53 pathway. In in vivo studies, AS-M not only suppresses the growth of human malignant brain tumors but also significantly prolongs patient survival. In addition, AS-M has potent anticancer effects involving cell cycle arrest, apoptosis, and antiangiogenesis. The in vitro and in vivo anticancer effects of AS-M indicate that this extract warrants further investigation and potential development as a new antibrain tumor agent, providing new hope for the chemotherapy of malignant brain cancer.

Lai, Wen-Lin; Harn, Horng-jyh; Hung, Pei-Hsiu; Hsieh, Ming-Chang; Chang, Kai-Fu; Huang, Xiao-Fan; Liao, Kuang-Wen; Lee, Ming-Shih; Tsai, Nu-Man

2013-01-01

136

Blockade of vascular angiogenesis by Aspergillus usamii var. shirousamii-transformed Angelicae Gigantis Radix and Zizyphus jujuba  

PubMed Central

The matrix metalloproteinases (MMP) play an important role in tumor invasion, angiogenesis and inflammatory tissue destruction. Increased expression of MMP was observed in benign tissue hyperplasia and in atherosclerotic lesions. Invasive cancer cells utilize MMP to degrade the extracellular matrix and vascular basement membrane during metastasis, where MMP-2 has been implicated in the development and dissemination of malignancies. The present study attempted to examine the antiangiogenic activity of the medicinal herbs of Aspergillus usamii var. shirousamii-transformed Angelicae Gigantis Radix and Zizyphus jujube (tAgR and tZj) with respect to MMP-2 production and endothelial motility in phorbol 12-myristate 13-acetate (PMA)- or VEGF-exposed human umbilical vein endothelial cells (HUVEC). Nontoxic tAgR and tZj substantially suppressed PMA-induced MMP-2 secretion. In addition, 25 µg/mL tAgR and tZj prevented vascular endothelial growth factor-stimulated endothelial cell transmigration and tube formation. The results reveal that tAgR and tZj dampened endothelial MMP-2 production leading to endothelial transmigration and tube formation. tAgR and tZj-mediated inhibition of endothelial MMP may boost a therapeutic efficacy during vascular angiogenesis.

Kang, Sang-Wook; Choi, Jung-Suk; Bae, Ji-Young; Li, Jing; Kim, Dong Shoo; Kim, Jung-Lye; Shin, Seung-Yong; You, Hyun Ju; Park, Hyoung-Sook; Ji, Geun Eog

2009-01-01

137

Comparison of ferulic acid content in Radix Angelicae Sinensis, Danggui-Buxue-Tang and Danggui-Sini-Tang  

PubMed Central

The aim of the present study was to determine the ferulic acid (FA) content of Radix Angelicae Sinensis (AS), Danggui-Buxue-Tang (DBT) and Danggui-Sini-Tang (DST) using the same ultra performance liquid chromatography system and method. FA was eluted using an Acquity BEH C18 column (100×2.1 mm inner diameter; 1.7 ?m). A mobile phase of methanol and 0.5% acetic acid was used and a flow rate of 0.3 ml/min was selected. The calibration curve exhibited a good linear regression (R2=0.9997). The inter- and intra-day precision measurements of FA ranged between 0.27 and 3.03% and the recovery ranged between 98.44 and 101.64% with relative standard deviation (RSD) values ?4.73%. The method was reliable and simple. The results of the chromatographic analyses indicate that the FA contents of the DBT and DST decoctions were increased compared with that of AS due to the presence of other herbs.

YAO, JIA-MEI; YU, LI-JIN; CHEN, QIONG; CHEN, ZE-QI; WANG, DONG-SHENG; QIU, XIN-JIAN; ZHAO, LIN-LIN

2014-01-01

138

[Structural analysis and anti-tumor activity in vivo of polysaccharide APS-2a from Angelica sinensis].  

PubMed

The polysaccharide APS-2a was isolated from Angelica sinensis (Oliv.) Diels through water extraction, deprotein, ethanol precipitation and DEAE-sephades A-25 column chromatography respectively,and was further purified by Sephacryl S-400 and Sephadex G-100 column chromatography. The phenol-sulfuric acid assay and Bradford method were used to determine the contents of carbohydrate and protein, respectively. The molecular weight was carried out with high-performance size exclusion chromatography (HPSEC) combined with a differential refractometer detector. The monosaccharide compositions were determined by gas chromatography after complete hydrolysis with acid. The models of mice transplanted sarcoma S-180 were used to study the anti-tumor effects in vivo. Thymus indexes, spleen indexes were determined. The HPSEC result showed the APS-2a was a single homogeneous component and its weight average molecular weight was 7.4 x 10(5) Da. The monosaccharide composition of APS-2a was glucose, galactose, arabinose, rhamnose, galcturonic acid. Furthermore, APS-2a (20.50 mg/kg) could inhibit the proliferation of tumor cells in mice transplanted S-180. The thymus indexes and spleen indexes in the groups treated with APS-2a were higher than control group. PMID:18619275

Cao, Wei; Li, Xiao-Qiang; Hou, Ying; Fan, Hui-Ting; Zhang, Xiao-Nan; Mei, Qi-Bing

2008-02-01

139

Supercritical fluid extraction for identification and determination of volatile metabolites from Angelica dahurica by GC-MS.  

PubMed

The volatile components of Angelica dahurica were obtained by supercritical fluid extraction (SFE) method. These oils obtained were analyzed by GC-MS (identification and determination of metabolites). The compounds were identified according to their retention indices and mass spectra (electron impact (EI), 70 eV). The effects of different parameters, such as pressure, temperature, flow rate of CO(2), and the amount of modifier, on the SFE of A. dahurica oil were investigated. A total of 50 compounds of SFE extracts were identified. Phellopterin (PO), isoimperatorin (IO), imperatorin (IM), alloimperatorin (AM), byakangelicin, isooxypeucedanin, and pimpinellin were the major coumarin compounds identified in A. dahurica SFE extracts. The quantitations of PO, IO, IM, and AM were then accomplished. The calibration curves showed good linearity (R(2) >0.99) in the concentration ranges tested. The recoveries were higher than 85%, with RSDs less than 10%. The GC-MS method was successfully validated and applied to the quantitation of A. dahurica. PMID:18705001

Chen, Qinhua; Li, Peng; He, Jing; Zhang, Zhuo; Liu, Jia

2008-10-01

140

Anti-asthmatic effects of Angelica dahurica against ovalbumin-induced airway inflammation via upregulation of heme oxygenase-1.  

PubMed

Asthma is a chronic immune inflammatory disease characterized by variable airflow obstruction. The present study was undertaken to assess the effects of an Angelica dahurica Bentham et Hooker ethanolic extract (AD) on airway inflammation in an ovalbumin (OVA)-induced airway inflammation model. Mice that received AD displayed significantly lower airway eosinophilia, cytokine levels, including interleukin (IL)-4, IL-5, and tumor necrosis factor (TNF)-alpha levels, mucus production and immunoglobulin (Ig)E, compared with OVA-induced mice. In our experiments, AD treatment reduced airway inflammation and suppressed oxidative stress in the OVA-induced asthma model, partly via induction of heme oxygenase (HO)-1. The effects of AD on OVA-induced HO-1 induction were partially reversed by the HO-1 inhibitor, tin protoporphyrin (SnPP). Our results clearly indicate that AD is a suppressor of airway allergic inflammation, and may thus be effectively used as an anti-inflammatory drug in the treatment of asthma. PMID:21146576

Lee, Mee-Young; Seo, Chang-Seob; Lee, Jin-Ah; Lee, Nam-Hun; Kim, Jung-Hoon; Ha, Heykyung; Zheng, Ming-Shan; Son, Jong-Keun; Shin, Hyeun-Kyoo

2011-04-01

141

A new procedure for the preparative separation and isolation of Z-ligustilide from the roots of Angelica sinensis.  

PubMed

A new procedure for the separation and isolation of Z-ligustilide from the roots of Angelica sinensis (AS) was developed, and the storage conditions for Z-ligustilide were optimized. Using the present procedure, Z-ligustilide was enriched by decomposing the Z-ligustilide dimers yielding Z-ligustilide and dissolving the polar impurities in hot water. Then, the crude Z-ligustilide was further purified by a semipreparative HPLC system. The spiked and nonspiked samples were used for the evaluation of the proposed procedure. Recoveries obtained varied from 86.2 to 90.7% and RSDs from 4.0 to 6.6%. The yield and purity of the isolated Z-ligustilide were found to be 4.57 mg/g and 99.6%, respectively. The results of stability tests have shown that the presence of oxidant contributes to Z-ligustilide degradation, therefore argon was chosen as a shielding gas for storage. The overall procedure is efficient and convenient which is considered suitable for the preparative separation of Z-ligustilide from AS. PMID:17638355

Yi, Tao; Zhang, Hao; Xie, Jing; Xue, Dan

2007-08-01

142

Radix Angelica Sinensis that contains the component Z-ligustilide promotes adult neurogenesis to mediate recovery from cognitive impairment.  

PubMed

Radix Angelica Sinensis (RAS) has beneficial effects in patients suffering from cognitive impairment associated with chronic cerebral hypoperfusion. It has previously been demonstrated that RAS prevents the neurotoxic effects of ?-amyloid (A?) in vitro, protects from injuries due to oxidative stress, inflammation and apoptosis and ameliorates scopolamine-induced amnesia in rats. Here, we studied the effects of RAS on cognitive improvement and neurogenic enhancement and examined the possible underlying mechanisms in a rat model with permanent bilateral common carotid artery occlusion, which serves as a model of chronic cerebral hypoperfusion-related neurodegenerative diseases. RAS enhances adult neurogenesis in the hippocampus following chronic cerebral hypoperfusion and improves the cognitive decline associated with hypoperfusion. Long-term ablation of adult hippocampal neurogenesis through cranial irradiation abolishes the protective effects of RAS on cognition. Furthermore, administration of RAS restores the decrease of brain-derived neurotrophic factor (BDNF) expression, the phosphorylation of cAMP-responsive element binding protein (CREB) and the glutamic acid decarboxylase 65 (GAD65) staining intensity in rats with chronic cerebral hypoperfusion. The present study supports the hypothesis that adult neurogenesis is required for RAS to facilitate recovery from the cognitive impairment induced by chronic cerebral hypoperfusion, while neurogenic enhancement secondary to RAS treatment may be due to increased BDNF and phosphorylated cAMP-responsive element binding protein (p-CREB) levels and increased ?-aminobutyric acid (GABA) expression. Based on the possible mechanisms suggested by the present study, this well-known traditional medicine may represent a candidate therapeutic agent for the treatment of dementia associated with vascular injury. PMID:23937197

Xin, Jiawei; Zhang, Junjian; Yang, Ying; Deng, Min; Xie, Xiaofeng

2013-11-01

143

Xanthoangelol and 4-Hydroxyderricin Are the Major Active Principles of the Inhibitory Activities against Monoamine Oxidases on Angelica keiskei K.  

PubMed

Monoamine oxidase inhibitors (MAOI) have been widely used as antidepressants. Recently, there has been renewed interest in MAO inhibitors. The activity-guided fractionation of extracts from Angelica keiskei Koidzumi (A. keiskei K.) led to the isolation of two prenylated chalcones, xanthoangelol and 4-hydroxyderricin and a flavonoid, cynaroside. These three isolated compounds are the major active ingredients of A. keiskei K. to inhibit the MAOs and DBH activities. Xanthoangelol is a nonselective MAO inhibitor, and a potent dopamine ?-hydroxylase (DBH) inhibitor. IC50 values of xanthoangelol to MAO-A and MAO-B were calculated to be 43.4 ?M, and 43.9 ?M. These values were very similar to iproniazid, which is a nonselective MAO inhibitor used as a drug against depression. The IC50 values of iproniazid were 37 ?M, and 42.5 ?M in our parallel examination. Moreover, IC50 value of xanthoangelol to DBH was calculated 0.52 ?M. 4-Hydroxyderricin is a potent selective MAO-B inhibitor and also mildly inhibits DBH activity. The IC50 value of 4-hydroxyderricin to MAO-B was calculated to be 3.43 ?M and this value was higher than that of deprenyl (0.046 ?M) used as a positive control for selective MAO-B inhibitor in our test. Cynaroside is a most potent DBH inhibitor. The IC50 value of cynaroside to DBH was calculated at 0.0410 ?M. Results of this study suggest that the two prenylated chalcones, xanthoangelol and 4-hydroxyderricin isolated from A. keiskei K., are expected for potent candidates for development of combined antidepressant drug. A. keiskei K. will be an excellent new bio-functional food material that has the combined antidepressant effect. PMID:24265870

Kim, Ji Ho; Son, Yeon Kyung; Kim, Gun Hee; Hwang, Keum Hee

2013-05-30

144

Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice  

PubMed Central

We examined whether Angelicae Dahuricae Radix (AR) suppresses the development of atopic dermatitis (AD)-like skin lesions induced by Dermatophagoides farinae in NC/Nga mice. To investigate the effect of AR, we measured the AD severity score, measured plasma levels of IgE and histamine, and performed histological analysis in NC/Nga mice. We also confirmed the anti-inflammatory effects of AR by measuring TARC/CCL17 production from LPS-treated RAW 264.7 cells and mRNA levels of TARC and MDC/CCL22 in TNF-?/IFN-?-treated HaCaT cells. 10?mg/day of AR extract was applied for 4 weeks to NC/Nga mice. Both the AR extract and 0.1% tacrolimus suppressed the development of AD-like skin lesions and reduced dermatitis scores of the back and ear skin. AR extracts caused an inhibition of histological changes induced by repeated application of D. farinae and a reduction of IgE and histamine levels in plasma (P < 0.05). Furthermore, NO production in LPS-treated RAW 264.7 cells was diminished in a dose-dependent manner, and hTARC production and TARC and MDC mRNA levels in TNF-?/IFN-?-treated HaCaT cells were diminished by AR. The inhibitory effect of AR on NO, TARC and MDC production may be associated with the suppression of AD-like skin lesions in D. farinae-induced NC/Nga mice.

Lee, Hoyoung; Lee, Jun Kyoung; Ha, Hyekyung; Lee, Mee-Young; Seo, Chang-Seob; Shin, Hyeun Kyoo

2012-01-01

145

Byakangelicol, isolated from Angelica dahurica, inhibits both the activity and induction of cyclooxygenase-2 in human pulmonary epithelial cells.  

PubMed

We examined the inhibitory mechanism of byakangelicol, isolated from Angelica dahurica, on interleukin-1beta (IL-1beta)-induced cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2) release in human pulmonary epithelial cell line (A549). Byakangelicol (10-50 microM) concentration-dependently attenuated IL-1beta-induced COX-2 expression and PGE2 release. The selective COX-2 inhibitor, NS-398 (0.01-1 microM), and byakangelicol (10-50 microM) both concentration-dependently inhibited the activity of the COX-2 enzyme. Byakangelicol, at a concentration up to 200 microM, did not affect the activity and expression of COX-1 enzyme. IL-1beta-induced p44/42 mitogen-activated protein kinase (MAPK) activation was inhibited by the MAPK/extracellular signal-regulated protein kinase (MEK) inhibitor, PD 98059 (30 microM), while byakangelicol (50 microM) had no effect. Treatment of cells with byakangelicol (50 microM) or pyrrolidine dithiocarbamate (PDTC; 50 microM) partially inhibited IL-1beta-induced degradation of IkappaB-alpha in the cytosol, translocation of p65 NF-kappaB from the cytosol to the nucleus and the NF-kappaB-specific DNA-protein complex formation. Taken together, we have demonstrated that byakangelicol inhibits IL-1beta-induced PGE2 release in A549 cells; this inhibition may be mediated by suppression of COX-2 expression and the activity of COX-2 enzyme. The inhibitory mechanism of byakangelicol on IL-1beta-induced COX-2 expression may be, at least in part, through suppression of NF-kappaB activity. Therefore, byakangelicol may have therapeutic potential as an anti-inflammatory drug on airway inflammation. PMID:12356282

Lin, C H; Chang, C W; Wang, C C; Chang, M S; Yang, L L

2002-09-01

146

Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E 2 and tumor necrosis factor-? via mitogen-activated protein kinases and nuclear factor-?B in macrophages  

Microsoft Academic Search

Angelica dahurica (Umbelliferae) has been used to treat headache of common cold, supraorbital neuralgia, painful swelling on the body, nasal stuffiness, leukorrhea and arthralgia due to wind-dampness in Korean traditional medicine. It is also claimed to be effective in the treatment of acne, erythema, headache, toothache, sinusitis, colds and flu. The present study focused whether the ethyl acetate extract from

Ok-Hwa Kang; Go-Hoon Lee; Hyuk Joon Choi; Pil Sang Park; Hee-Sung Chae; Seung-Il Jeong; Youn-Chul Kim; Dong Hwan Sohn; Hyun Park; John Hwa Lee; Dong-Yeul Kwon

2007-01-01

147

Coniferyl Ferulate, a Strong Inhibitor of Glutathione S-Transferase Isolated from Radix Angelicae sinensis, Reverses Multidrug Resistance and Downregulates P-Glycoprotein  

PubMed Central

Glutathione S-transferase (GST) is the key enzyme in multidrug resistance (MDR) of tumour. Inhibition of the expression or activity of GST has emerged as a promising therapeutic strategy for the reversal of MDR. Coniferyl ferulate (CF), isolated from the root of Angelica sinensis (Oliv.) Diels (Radix Angelicae sinensis, RAS), showed strong inhibition of human placental GST. Its 50% inhibition concentration (IC50) was 0.3??M, which was greater than a known GSTP1-1 inhibitor, ethacrynic acid (EA), using the established high-throughput screening model. Kinetic analysis and computational docking were used to examine the mechanism of GST inhibition by CF. Computational docking found that CF could be fully docked into the gorge of GSTP1-1. The further exploration of the mechanisms showed that CF was a reversible noncompetitive inhibitor with respect to GSH and CDNB, and it has much less cytotoxicity. Apoptosis and the expression of P-gp mRNA were evaluated in the MDR positive B-MD-C1 (ADR+/+) cell line to investigate the MDR reversal effect of CF. Moreover, CF showed strong apoptogenic activity and could markedly decrease the overexpressed P-gp. The results demonstrated that CF could inhibit GST activity in a concentration-dependent manner and showed a potential MDR reversal effect for antitumour adjuvant therapy.

Chen, Chang; Wu, Chuanhong; Lu, Xinhua; Yan, Zhiyong; Gao, Jian; Zhao, Hui; Li, Shaojing

2013-01-01

148

Coniferyl Ferulate, a Strong Inhibitor of Glutathione S-Transferase Isolated from Radix Angelicae sinensis, Reverses Multidrug Resistance and Downregulates P-Glycoprotein.  

PubMed

Glutathione S-transferase (GST) is the key enzyme in multidrug resistance (MDR) of tumour. Inhibition of the expression or activity of GST has emerged as a promising therapeutic strategy for the reversal of MDR. Coniferyl ferulate (CF), isolated from the root of Angelica sinensis (Oliv.) Diels (Radix Angelicae sinensis, RAS), showed strong inhibition of human placental GST. Its 50% inhibition concentration (IC50) was 0.3? ? M, which was greater than a known GSTP1-1 inhibitor, ethacrynic acid (EA), using the established high-throughput screening model. Kinetic analysis and computational docking were used to examine the mechanism of GST inhibition by CF. Computational docking found that CF could be fully docked into the gorge of GSTP1-1. The further exploration of the mechanisms showed that CF was a reversible noncompetitive inhibitor with respect to GSH and CDNB, and it has much less cytotoxicity. Apoptosis and the expression of P-gp mRNA were evaluated in the MDR positive B-MD-C1 (ADR+/+) cell line to investigate the MDR reversal effect of CF. Moreover, CF showed strong apoptogenic activity and could markedly decrease the overexpressed P-gp. The results demonstrated that CF could inhibit GST activity in a concentration-dependent manner and showed a potential MDR reversal effect for antitumour adjuvant therapy. PMID:24058374

Chen, Chang; Wu, Chuanhong; Lu, Xinhua; Yan, Zhiyong; Gao, Jian; Zhao, Hui; Li, Shaojing

2013-01-01

149

The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia.  

PubMed

A crude polysaccharide was obtained by water extraction and ethanol precipitation from the root of Angelica sinensis (AS) to investigate its suppressive effect on hepcidin expression in rats with iron deficiency anemia (IDA). In this study, we showed that the Angelica sinensis polysaccharide (ASP) was mainly composed of arabinose, glucose and galactose in a molar ratio of 1?:?2.5?:?7.5, with the molecular weight of 72?900 Da and the sugar content of 95.1%. ASP markedly decreased hepcidin expression by suppressing the expression of JAK1/2, phospho-JAK1/2, phospho-SMAD1/5/8, phospho-ERK1/2, and promoting the expression of SMAD7 in the liver. These findings suggest that ASP can prevent the janus-kinase (JAK), son of mother against decapentaplegic (SMAD) and extracellular signal-regulated kinase (ERK) pathways to down-regulate hepcidin expression in IDA rats, and may be useful for the treatments of diseases induced by hepcidin over-expression. PMID:24752529

Zhang, Yu; Cheng, Yao; Wang, Na; Zhang, Qilin; Wang, Kaiping

2014-06-25

150

Preparative isolation and purification of coumarins from Angelica dahurica (Fisch. ex Hoffn) Benth, et Hook. f (Chinese traditional medicinal herb) by high-speed counter-current chromatography.  

PubMed

A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of coumarins from Angelica dahurica (Fisch. ex Hoffin) Benth, et Hook. f(Baizhi in Chinese) was successfully established by using n-hexane-methanol-water as the two-phase solvent system in gradient elution mode. The upper phase of n-hexane-methanol-water (5:5:5, v/v) was used as the stationary phase of HSCCC. The mobile phase used in HSCCC was the lower phase of n-hexane-methanol-water (5:5:5, v/v) and n-hexane-methanol-water (5:7:3, v/v) that was changed in gradient. Three major components including imperatorin, isoimperatorin and oxypeucedanine were isolated, each at over 98% purity as determined by high-performance liquid chromatography (HPLC). The peak fractions of HSCCC were identified by 1H-NMR and 13C-NMR. PMID:15527142

Liu, Renmin; Li, Aifeng; Sun, Ailing

2004-10-15

151

Bioactivity-guided fractionation of the volatile oil of Angelica sinensis radix designed to preserve the synergistic effects of the mixture followed by identification of the active principles.  

PubMed

In natural product research, it is a common experience that fractionation of biologically-active crude extracts can lead to the loss of their original activity. This is attributed to synergistic effects, where two or more components are required to be present together for full activity of the sample. Our previous study showed that a volatile oil of Angelica sinensis radix (VOAS) inhibited endothelial cell proliferation in culture. Here we have used a bioactivity-guided fractionation method to preserve any synergistic effects of VOAS combining countercurrent chromatography (CCC), the MTS cell viability assay and gas chromatography (GC). Using a two-phase CCC solvent system (heptane-ethyl acetate-methanol-water at a volume ratio of 27:23:27:23%), forty-five fractions were isolated, nine of which exhibited anti-endothelial properties. GC analysis showed two bioactive alkylphthalides, Z-ligustilide and n-butylidenephthalide (BP) were the major compounds detected in the bioactive fractions, and were absent in non-bioactive fractions. Our results indicate that Z-ligustilide and BP are the main constituents responsible for the anti-endothelial properties of VOAS. This rapid and reliable approach in preserving sample activity while isolating and identifying its active compounds suggests that this protocol can be a powerful tool for drug discovery from natural products. PMID:22458966

Yeh, Ju-Ching; Garrard, Ian J; Cho, Chin-Wen Chantal; Annie Bligh, S W; Lu, Guang-hua; Fan, Tai-Ping; Fisher, Derek

2012-05-01

152

Separation and quantitative analysis of coumarin compounds from Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook. f by pressurized capillary electrochromatography.  

PubMed

A pressurized capillary electrochromatography (pCEC) method with post-column detection cell has been developed for the therapeutically important coumarins from Angelica dahurica extract. The separation of five major coumarins (xanthotoxol, osthenol, imperatorin, oxypeucedanin hydrate, byakangelicin) was optimized with respect to composition of the mobile phase, ionic strength of buffers, pH, and applied voltage. Baseline separation was achieved for the five coumarins in less than 25 min using a mobile phase of methanol-acetonitrile-phosphate buffer (pH 4.8; 15 mM) (22.5:15:62.5, v/v/v). The method showed satisfactory retention time and peak area repeatability with the first use of post-column detection cell in the pCEC instrument. Comparing to capillary high performance liquid chromatography (capillary HPLC) and conventional high performance liquid chromatography (HPLC), higher column efficiency, and shorter analysis time were achieved in pCEC. The five coumarins in the extract samples representing different stages of traditional extraction of A. dahurica were also quantitatively analyzed by pCEC. The calibration curves were linear in the range 37-129, 36-126, 12-41, 88-306, 20-69 microg/ml of the standard solutions containing the five coumarins with correlation coefficients between 0.9976 and 0.9994. PMID:16318908

Chen, Yi; Fan, Guorong; Chen, Bin; Xie, Ying; Wu, Huiling; Wu, Yutian; Yan, Chao; Wang, Jianmin

2006-04-11

153

Anti-inflammatory activity of Angelica dahurica ethanolic extract on RAW264.7 cells via upregulation of heme oxygenase-1.  

PubMed

In this study, we analyzed the anti-inflammatory effects of Angelica dahurica Bentham et Hooker ethanolic extract (ADEE) on RAW264.7 cells, to understand the mechanism underlying its observed effects. ADEE inhibited cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to the suppression of COX-2-derived prostaglandin E(2) and iNOS-derived production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. These inhibitory effects of ADEE were accompanied by the reduced production of tumor necrosis factor ? and interleukin (IL)-6. ADEE also inhibited nuclear factor ?B (NF?B) translocation to the nucleus by interrupting inhibitor kappa B? (I?B?) degradation. ADEE upregulated heme oxygenase 1 expression, and treatment with tin protoporphyrin IX (SnPP), a selective inhibitor of HO-1, reversed the LPS-induced generation of proinflammatory cytokines. ADEE also induced IL-4 and IL-5 expression in concanavalin-A-stimulated splenocytes. These results suggest that ADEE has anti-inflammatory activity, which acts via the suppression of the NF-?B pathway. PMID:21272610

Lee, Mee-Young; Lee, Jin-Ah; Seo, Chang-Seob; Ha, Heykyung; Lee, Hoyoung; Son, Jong-Keun; Shin, Hyeun-Kyoo

2011-05-01

154

Computer-assisted, high-performance liquid chromatography with mass spectrometric detection for the analysis of coumarins in Peucedanum palustre and Angelica archangelica.  

PubMed

A reversed-phase HPLC method with atmospheric pressure chemical ionisation MS detection has been developed for the separation and identification of coumarins in plants of Peucedanum palustre L. (Moench) and Angelica archangelica (L.) var. archangelica. The Turbo Method Development program was utilised to optimise the mobile phase with two organic solvents (acetonitrile and methanol) and two aqueous solutions (1.0% formic acid and 10 mM ammonium acetate). Optimisation of the solvent gradients for the method was performed with the aid of the DryLab program. Analyses were carried out using a Phenomenex Prodigy RP C18 column. Fifty-two peaks (14 of which were associated with coumarins) were separated in 30 min from extracts of P. palustre, and 48 peaks (15 associated with coumarins) from extracts of A. archangelica. A total of 21 different coumarin-type compounds were identified in the aerial and the underground parts of the title plants. Isopimpinellin and pimpinellin were found for the first time in P. palustre and were identified by comparison of retention times and MS data obtained following the analysis of pure standards. This is the first report of the coumarin composition of the umbels of P. palustre. PMID:15202601

Eeva, Manu; Rauha, Jussi-Pekka; Vuorela, Pia; Vuorela, Heikki

2004-01-01

155

Anti-Inflammatory activity of Angelica keiskei through suppression of mitogen-activated protein kinases and nuclear factor-kappaB activation pathways.  

PubMed

Angelica keiskei has been shown to exhibit antitumor, antioxidant, and antidiabetic activities, and the fresh leaves and dry powder are used for health food. In spite of several beneficial effects, however, the molecular mechanism or mechanisms behind anti-inflammatory activities of A. keiskei remain unclear. Thus, we investigated the effects of A. keiskei on the activities of inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. We found that the n-hexane fraction of A. keiskei (HAK) significantly inhibited LPS-induced NO and prostaglandin E(2) production and tumor necrosis factor-alpha secretion. HAK also inhibited the expression of LPS-induced iNOS and COX-2 proteins and their mRNA levels. Furthermore, we hypothesize that anti-inflammatory effects by HAK can be linked to interference with the signaling pathway of mitogen-activated protein kinases (MAPKs) and the activation pathway of nuclear factor kappaB (NF-kappaB). HAK suppressed LPS-induced c-Jun NH(2)-terminal kinase, p38, and p44/p42 MAPK activation. We also found that the cell-based assay system showed that HAK suppressed LPS-induced NF-kappaB activity in transfectant RAW 264.7 cells. In addition, the electrophoretic mobility shift assay showed the same result as in the cell-based assay system. Our data suggest that the anti-inflammatory effect of HAK is mediated through down-modulation of iNOS and COX-2 gene products by blocking the signaling pathways of MAPKs and NF-kappaB. PMID:20521991

Lee, Hyoung Joo; Choi, Tae Won; Kim, Hyun Jung; Nam, Dongwoo; Jung, Sang Hoon; Lee, Eun Ha; Lee, Hee Ju; Shin, Eun Myoung; Jang, Hyeung-Jin; Ahn, Kyoo Seok; Shim, Bum Sang; Choi, Seung-Hoon; Kim, Sung-Hoon; Sethi, Gautam; Ahn, Kwang Seok

2010-06-01

156

Identification and comparative determination of senkyunolide A in traditional Chinese medicinal plants Ligusticum chuanxiong and Angelica sinensis by HPLC coupled with DAD and ESI-MS.  

PubMed

Using the HPLC/DAD/ESI/MS method, the qualitative and quantitative analysis of senkyunolide A (SA) in the rhizomes of Ligusticum chuanxiong (Rhizoma chuanxiong; CX) and roots of Angelica sinensis (DG) was established. As a result, it was found that SA is a characteristic standard compound for the quality evaluation and chemical differentiation between CX and DG. Methanol was chosen in the preparation of standard solutions and extraction of samples based on the stability data. The identity of SA in CX and DG was unambiguously determined based on the quasimolecular ions in ESI-MS. A comprehensive validation of the method, including sensitivity, linearity, reproducibility and recovery, was conducted using the optimized chromatographic conditions. The linear calibration curve was acquired with R2>0.999 and limit of detection (S/N=3) was estimated to be 12.5 mug/g. The reproducibility was evaluated by repeated sample injection and replicated analysis of samples with the relative standard deviation (RSD) value found within 0.68%. The recovery rates of SA varied within the range of 96.91-101.50% with RSD less than 2.38%. In the present work, the contents of SA were quantified within 3.94-9.14 mg/g and 0.108-0.588 mg/g for 12 batches each of CX and DG. The results demonstrated that SA is a useful standard compound for the quality evaluation and chemical differentiation between CX and DG. The analytical procedure is precise and reproducible and thus suitable for the analysis of a large number of samples. PMID:16272738

Yi, Tao; Leung, Kelvin Sze-Yin; Lu, Guang-Hua; Zhang, Hao; Chan, Kelvin

2005-11-01

157

Chemical changes of Angelicae Sinensis Radix and Chuanxiong Rhizoma by wine treatment: chemical profiling and marker selection by gas chromatography coupled with triple quadrupole mass spectrometry  

PubMed Central

Background Angelicae Sinensis Radix (ASR) and Chuanxiong Rhizoma (CR) can be treated with wine to promote their biological functions in Chinese medicine. Both ASR and CR contain similar volatile chemicals that could be altered after wine treatment. This study aims to identify the differential chemical profiles and to select marker chemicals of ASR and CR before and after wine treatment. Methods Chemical analyses were carried out by gas chromatography-triple quadrupole mass spectrometry (GC-QQQ-MS/MS) coupled with multivariate statistical analysis. Characterization of the compositions of essential oils was performed by automated matching to the MS library and comparisons of their mass spectra (NIST08 database). For ferulic acid, butylphthalide, Z-butylidenephthalide, senkyunolide A and Z-ligustilide, the mass spectrometer was operated in electron ionization mode, the selection reaction monitoring mode was used and an evaluation of the stability and sensitivity of the chromatographic system was performed for the tested extraction. Results Principal component analysis (PCA) simultaneously distinguished ASR and CR from different forms. Ferulic acid, Z-butylidenephthalide, Z-ligustilide, butylphthalide and senkyunolide A were screened by PCA loading plots and can be used as chemical markers for discrimination among different groups of samples. Conclusion Different chemical profiles of ASR and CR after wine treatment could be identified by GC-QQQ-MS/MS. The five marker chemicals selected by PCA, namely ferulic acid, butylphthalide, Z-butylidenephthalide, senkyunolide A and Z-ligustilide, were sufficient to distinguish between the crude and corresponding wine-treated forms of ASR and CR.

2013-01-01

158

[Interaction of nourishing and tonifying blood effects of the combination of Angelicae sinensis Radix and Astragali Radix studied by response surface method].  

PubMed

The combination of Angelicae sinensis Radix (Danggui, DG) and Astragali Radix (Huangqi, HQ) is a popular herb pair commonly used in clinic for the treatment of blood deficiency syndrome in China. The aim of this paper is to study the interaction of DG and HQ nourishing and tonifying blood effects by response surface method. The blood deficiency mice were induced by injecting N-acetylphenylhydrazine (sc) and cyclophosphamide (ip). The blood deficiency mice were administrated intragastrically with DG-HQ extracts (0:1, 1: 5, 2:5, 2:3, 1:1, 3:2, 5:2, 5:1, 1:0). The changes of the peripheral blood indexes and organ indexes were observed. The indexes were integrated by comprehensive index method; the interactions of DG and HQ were analyzed by the response surface diagram established with Matlab software. The results showed that DG and HQ at most of their combination ratios had synergic effect. Within the range of 1:5 - 5:1, all of the extracts of DG-HQ showed synergic effect, and among which, high-doses had better effects than low-doses. The highest value (-1) of the synergic effect was showed when DG was 10 - 40 g at the same time of HQ as 90 -180 g, and DG was 50 - 100 g at the same time of HQ as 20 - 100 g. DG-HQ at all combination dosages within Chinese Pharmacopeia (DG: 6 - 12 g, HQ: 9 - 30 g) had certain synergic effect, and Danggui Buxue Decoction (DG: 6 g, HQ: 30 g) also was at this range. The results provided scientific basis to the clinical application of DG and HQ. And the response surface method was firstly applied to quantitatively evaluate the bio-activity change of herb combination, which provided a novel way for modern basic research on the interaction of herbs. PMID:23289152

Shi, Xu-qin; Shang, Er-xin; Tang, Yu-ping; Zhu, Hua-xu; Guo, Jian-ming; Huang, Mei-yan; Li, Wei-xia; Duan, Jin-ao

2012-10-01

159

Effects of natural phytochemicals in Angelica sinensis (Danggui) on Nrf2-mediated gene expression of phase II drug metabolizing enzymes and anti-inflammation.  

PubMed

The root of Angelica sinensis (Oliv.) Diels (abbreviated as AS) (Danggui) has a long history in Asian herbal medicine. Recently, it was demonstrated that AS possesses anti-cancer and anti-oxidant activities. Because the transcription factor Nrf2 mediates the expression of many cellular anti-oxidative stress genes, including genes that are involved in phase II drug metabolism and anti-oxidative stress, this study sought to investigate whether pure compounds from AS or an AS extract could activate antioxidant response element (ARE)-mediated gene expression and induce anti-inflammatory activities. Z-Ligustilide (Ligu), 3-butylidenephthalide (Buty) and CO2 supercritical fluid-extracted lipophilic AS extract (SFE) were tested in HepG2-C8 cells stabilized with ARE luciferase reporter gene. Ligu and Buty caused significant toxicity only at 100 ?m. All three samples induced ARE-luciferase activity; however, SFE at 8.5 µg/ml induced ARE-luciferase activity 2-3 fold more potently than did either of the pure compounds. SFE also significantly increased the endogenous mRNA of Nrf2 and the Nrf2 target anti-oxidative gene NAD(P)H dehydrogenase, quinone 1 (NQO1). The protein expression of NQO1 was also significantly induced by SFE. In RAW 264.7 cells, SFE suppressed lipopolysaccharide (LPS)-induced IL-1? and TNF-? expression about 2 fold stronger than sulforaphane, whereas both pure compounds and SFE suppressed inflammatory nitric oxide (NO) production. In summary, this study demonstrates that AS has anti-inflammatory effects and activates the Nrf2 pathway, which protects against oxidative stress. PMID:23640758

Saw, Constance Lay Lay; Wu, Qing; Su, Zheng-Yuan; Wang, Hu; Yang, Yinhua; Xu, Xiaoting; Huang, Ying; Khor, Tin Oo; Kong, Ah-Ng Tony

2013-09-01

160

The natural compound n-butylidenephthalide derived from the volatile oil of Radix Angelica sinensis inhibits angiogenesis in vitro and in vivo.  

PubMed

Radix Angelica sinensis is a Chinese medicinal herb that has been used extensively in the East for the treatment of cardiovascular diseases (CVDs). Angiogenesis plays an important role in the pathogenesis of CVDs. We hypothesized that Radix A. sinensis may contain angiogenesis modulators. In the current study, we investigated the effects of a volatile oil of Radix A. sinensis (VOAS) and n-butylidenephthalide (BP), one of the bioactive components in VOAS, on angiogenesis in vitro and in vivo. The results suggested that VOAS exerted anti-angiogenic effects by inhibiting human umbilical vein endothelial cell proliferation, migration and capillary-like tube formation on Matrigel. BP was also shown to be anti-angiogenic and its mechanisms were through inhibition of cell cycle progression and induction of apoptosis. Western blotting analysis indicated that the anti-angiogenic actions of BP were associated with the activation of p38 and ERK 1/2 but not SAPK/JNK and Akt signaling pathways. Further investigations showed that BP inhibited endothelial sprouting in an ex vivo mouse aortic ring model and was a potent inhibitor of the development of zebrafish subintestinal vessels in vivo. Our data using the volatile oil contrast with previous findings, which showed an aqueous extract of Radix A. sinensis was pro-angiogenic. This highlights the importance of identifying pro- and anti-angiogenic substances in Radix A. sinensis, not only for the development of novel angiogenesis modulators for the treatment of CVDs, but also to ensure the proper use of Radix A. sinensis as a nutraceutical. PMID:21327473

Yeh, Ju-Ching; Cindrova-Davies, Tereza; Belleri, Mirella; Morbidelli, Lucia; Miller, Nigel; Cho, Chin-Wen Chantal; Chan, Kelvin; Wang, Yi-Tao; Luo, Guo-An; Ziche, Marina; Presta, Marco; Charnock-Jones, David Stephen; Fan, Tai-Ping

2011-05-01

161

Pharmacokinetic Comparison of Ferulic Acid in Normal and Blood Deficiency Rats after Oral Administration of Angelica sinensis, Ligusticum chuanxiong and Their Combination  

PubMed Central

Radix Angelica Sinensis (RAS) and Rhizome Ligusticum (RLC) combination is a popular herb pair commonly used in clinics for treatment of blood deficiency syndrome in China. The aim of this study is to compare the pharmacokinetic properties of ferulic acid (FA), a main bioactive constituent in both RAS and RLC, between normal and blood deficiency syndrome animals, and to investigate the influence of compatibility of RAS and RLC on the pharmacokinetic of FA. The blood deficiency rats were induced by injecting 2% Acetyl phenylhydrazine (APH) on the first day, every other day, to a total of five times, at the dosage of 100, 50, 50, 30, 30 mg/kg body mass, respectively. Quantification of FA in rat plasma was achieved by using a simple and rapid HPLC method. Plasma samples were collected at different time points to construct pharmacokinetic profiles by plotting drug concentration versus time, and estimate pharmacokinetic parameters. Between normal and blood deficiency model groups, both AUC(0–t) and Cmax of FA in blood deficiency rats after RAS-RLC extract administration increased significantly (P < 0.05), while clearance (CL) decreased significantly. Among three blood deficiency model groups, t1/2?, Vd, AUC(0–t) and AUC(0–?) all increased significantly in the RAS-RLC extract group compared with the RAS group. The results indicated that FA was absorbed better and eliminated slower in blood deficiency rats; RLC could significantly prolong the half-life of distribution, increase the volume of distribution and the absorption amount of FA of RAS in blood deficiency rats, which may be due to the synergic action when RAS and RLC were used together to treat blood deficiency syndrome.

Li, Weixia; Guo, Jianming; Tang, Yuping; Wang, Huan; Huang, Meiyan; Qian, Dawei; Duan, Jin-Ao

2012-01-01

162

The natural compound n-butylidenephthalide derived from Angelica sinensis inhibits malignant brain tumor growth in vitro and in vivo3  

PubMed Central

The naturally-occurring compound, n-butylidenephthalide (BP), which is isolated from the chloroform extract of Angelica sinensis (AS-C), has been investigated with respect to the treatment of angina. In this study, we have examined the anti-tumor effects of n-butylidenephthalide on glioblastoma multiforme (GBM) brain tumors both in vitro and in vivo. In vitro, GBM cells were treated with BP, and the effects of proliferation, cell cycle and apoptosis were determined. In vivo, DBTRG-05MG, the human GBM tumor, and RG2, the rat GBM tumor, were injected subcutaneously or intracerebrally with BP. The effects on tumor growth were determined by tumor volumes, magnetic resonance imaging and survival rate. Here, we report on the potency of BP in suppressing growth of malignant brain tumor cells without simultaneous fibroblast cytotocixity. BP up-regulated the expression of Cyclin Kinase Inhibitor (CKI), including p21 and p27, to decrease phosphorylation of Rb proteins, and down-regulated the cell-cycle regulators, resulting in cell arrest at the G0/G1 phase for DBTRG-05MG and RG2 cells, respectively. The apoptosis-associated proteins were dramatically increased and activated by BP in DBTRG-05MG cells and RG2 cells, but RG2 cells did not express p53 protein. In vitro results showed that BP triggered both p53-dependent and independent pathways for apoptosis. In vivo, BP not only suppressed growth of subcutaneous rat and human brain tumors but also, reduced the volume of GBM tumors in situ, significantly prolonging survival rate. These in vitro and in vivo anti-cancer effects indicate that BP could serve as a new anti-brain tumor drug.

Tsai, Nu-Man; Chen, Yi-Lin; Lee, Chau-Chin; Lin, Po-Chen; Cheng, Yeung-Leung; Chang, Wen-Liang; Lin, Shinn-Zong; Harn, Horng-Jyh

2006-01-01

163

Effects of zinc and cadmium ions on cell growth and production of coumarins in cell suspension cultures of Angelica archangelica L.  

PubMed

The plant cell may respond to the excess of heavy metals in its environment by various mechanisms, including enhanced biosynthesis of secondary metabolites. In this study, zinc (0 to 1500 ?M) and cadmium ions (0 to 100 ?M) were tested as potential elicitors of the production of coumarins in angelica cell suspension cultures. In addition, the toxicity of both metals was assessed by evaluating their effect on cell growth (characterized by fresh and dry biomass at the end of a two-week subculture). It has been found that fresh biomass was not influenced up to zinc concentrations of 150 and 300 ?M in the dark-grown and light-grown cultures, resp. Then it declined with an increasing zinc level. Zinc at 1500 ?M diminished it by 54% and 24% in the dark-grown and light-grown cultures, resp. Dry biomass was influenced in a similar way. Zinc at 1500 ?M reduced dry cell weight by 30% and 20% in cultures in the dark and in the light, resp. Cadmium ions did not affect fresh and dry weights of cells up to concentrations of 10 ?M and 50 ?M in cultures in the dark and in the light, resp. Toxic concentrations of cadmium are by an order of magnitude lower than those of zinc. Cadmium at 50 ?M reduced fresh and dry cell weights by 66% and 59%, resp., in the dark-grown cultures. Cadmium at 100 ?M caused a decrease in fresh and dry biomass by 40% and 44%, resp., in the light-grown cultures. Neither zinc nor cadmium improved production of coumarins. PMID:23387854

Siatka, Tomáš; Kašparová, Marie; Spilková, Ji?ina

2012-12-01

164

Angelica sinensis and its Alkylphthalides Induce the Detoxification enzyme NAD(P)H: Quinone OxidoReductase 1 by Alkylating KEAP1  

PubMed Central

The roots of Angelica sinensis (Oliv.), Diels (Dang Gui; Apiaceae) have a long history in traditional Chinese medicine as a remedy for women's disorders, and are often called “lady's ginseng”. Currently, extracts of A. sinensis are commonly included in numerous dietary supplements used for women's health and as anti-aging products. In the present study, we examined the potential chemopreventive activity of A. sinensis extracts by measuring the relative ability to induce the detoxification enzyme, NAD(P)H:quinone oxidoreductase 1 (NQO1). The lipophilic partitions showed strong NQO1 induction with concentrations to double the enzyme activity (CD) of 5.5 ± 0.7 ?g/mL (petroleum ether) and 3.9 ± 0.5 ?g/mL (chloroform). Fractionation led to the isolation of phenolic esters and alkylphthalides, especially Z-ligustilide, the main lipophilic compound, which showed strong NQO1 inducing properties (CD = 6.9 ± 1.9 ?M). Transcription of many detoxifying enzymes is regulated through the antioxidant response element (ARE) and its transcription factor Nrf2, which is repressed under basal conditions by Keap1. However, exposure to electrophilic inducers that alkylate Keap1 results in a higher concentrations of free Nrf2 and ARE activation. The ARE reporter activity was therefore analyzed in HepG2-ARE-C8 cells after incubation with lipophilic extracts of A. sinensis or ligustilide for 24 h. Under these conditions, both the extract and ligustilide increased ARE-luciferase reporter activity in a dose-dependent manner. Incubation of ligustilide with GSH and subsequent LC-MS-MS analysis revealed that ligustilide as well as oxidized ligustilide species covalently modified GSH. In addition, using MALDI-TOF mass spectrometry and LC-MS-MS, it was demonstrated that the lipophilic extracts, ligustilide, and monooxygenated ligustilide alkylated important cysteine residues in human Keap1 protein, thus activating Nrf2 and transcription of ARE regulated genes. These observations suggest that A. sinensis dietary supplements standardized to ligustilide have potential as chemopreventive agents through induction of detoxification enzymes.

Dietz, Birgit M.; Liu, Dongting; Hagos, Ghenet K.; Yao, Ping; Schinkovitz, Andreas; Pro, Samuel M.; Deng, Shixin; Farnsworth, Norman R.; Pauli, Guido F.; van Breemen, Richard B.; Bolton, Judy L.

2008-01-01

165

Simultaneous determination of bioactive components of Radix Angelicae Sinensis-Radix Paeoniae Alba herb couple in rat plasma and tissues by UPLC-MS/MS and its application to pharmacokinetics and tissue distribution.  

PubMed

A highly sensitive and rapid ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been developed and validated for simultaneous quantification of seven components in rat plasma and five components in rat tissues after oral administration of the extracts of different combination Radix Angelicae Sinensis-Radix Paeoniae Alba herb couple and has been applied to compare the different pharmacokinetics and tissue distribution properties of these bioactive components. The extracts of Radix Angelicae Sinensis (RAS), Radix Paeoniae Alba (RPA) and Radix Angelicae Sinensis-Radix Paeoniae Alba herb couple (RRHC) were orally administrated to rats, respectively. The concentrations of ferulic acid, caffeic acid, vanillic acid, ligustilide, paeoniflorin, albiflorin and oxypaeoniflorin in rat plasma and the concentrations of ferulic acid, vanillic acid, paeoniflorin, albiflorin and oxypaeoniflorin in tissues were determined by UPLC-MS/MS. The plasma samples were pretreated by protein precipitation with methanol and the tissue samples were homogenated with water and pretreated by protein precipitation with methanol. Chromatographic separation was performed on a C18 column using 0.1% formic acid-acetonitrile as mobile phase for gradient elution. A triple quadrupole (TQ) tandem mass spectrometry equipped with an electrospray ionization source was used as detector operating both in positive and negative ionization mode and operated by multiple-reaction monitoring (MRM) scanning. Noncompartmental pharmacokinetic parameters were calculated by DAS 2.0 program. The differences between each group were compared by SPSS 16.0 with Independent-Samples T-test. The pharmacokinetic parameters (such as Cmax, Tmax, T1/2, AUC0-T, MRT0-T, Vz/F or CLz/F) of all the detected components between the single herb (RAS or RPA) and herb pair (RRHP) showed significant differences (P<0.05). It indicated that the compatibility of RAS and RPA could alter the pharmacokinetics features of each component. Tissue distribution results showed that ferulic acid, vanillic acid, paeoniflorin, albiflorin and oxypaeoniflorin mostly distributed in liver and kidney both in herb couple and single herb distributed most in liver and kidney. Compared with single herb, RRHC could increase or decrease the concentrations of five components at different time points compared with the sing herb. The results indicated the method was successfully applied to the comparative study on pharmacokinetics and tissue distribution of different combination of RRHC in rats. The compatibility of two Chinese herbs could alter the pharmacokinetics and tissue distribution properties of major bio-active components in the single herb. The results might be helpful for further investigation of compatibility mechanism of RRHC. PMID:24927419

Luo, Niancui; Li, Zhenhao; Qian, Dawei; Qian, Yefei; Guo, Jianming; Duan, Jin-Ao; Zhu, Min

2014-07-15

166

Preparative isolation of imperatorin, oxypeucedanin and isoimperatorin from traditional Chinese herb "bai zhi"Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook using multidimensional high-speed counter-current chromatography.  

PubMed

Preparative high-speed counter-current chromatography (CCC) was successfully used for isolation and purification of imperatorin, oxypeucedanin and isoimperatorin from traditional Chinese herb "bai zhi"Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook using multidimensional counter-current chromatography with a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water at volume ratios of 1:1:1:1 (v/v) and 5:5:4.5:5.5 (v/v), which had been selected by analytical high-speed counter-current chromatography (HSCCC). Using two preparative units of the CCC centrifuge, which are connected by a column switching valve, about a 300 mg amount of the crude extract was separated, yielding 19.9 mg of imperatorin, 8.6 mg of oxypeucedanin and 10.4 mg of isoimperatorin all at a high purity of over 98%. PMID:16542667

Wei, Yun; Ito, Yoichiro

2006-05-19

167

5-Methoxy-8-(2-hydroxy-3-buthoxy-3-methylbutyloxy)-psoralen isolated from Angelica dahurica inhibits cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells.  

PubMed

5-Methoxy-8-(2-hydroxy-3-buthoxy-3-methylbutyloxy)-psoralen (MP) is a medicinal herbal product isolated from Angelica dahurica that inhibits the cyclooxygenase-2 (COX-2)-dependent phase of prostaglandin D(2) (PGD(2)) generation in bone marrow-derived mast cells (IC(50), 23.5 microM). Western blotting with specific anti-COX-2 antibodies showed that the decrease in PGD(2) production was accompanied by a decrease in COX-2 protein levels. In addition, this compound consistently inhibited the production of leukotriene C(4) in a dose dependent manner, (IC(50), 2.5 microM). These results demonstrate that MP inhibits both cyclooxygenase-2 and 5-lipoxygenase activity. Furthermore, this compound also inhibited the degranulation reaction (IC(50), 4.1 microM). Therefore, this compound might provide a basis for novel anti-inflammatory drug development. PMID:18481018

Hua, Jin Mei; Moon, Tae Chul; Hong, Tae Gyun; Park, Kyong Min; Son, Jong Keun; Chang, Hyeun Wook

2008-05-01

168

Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E2 and tumor necrosis factor-alphavia mitogen-activated protein kinases and nuclear factor-kappaB in macrophages.  

PubMed

Angelica dahurica (Umbelliferae) has been used to treat headache of common cold, supraorbital neuralgia, painful swelling on the body, nasal stuffiness, leukorrhea and arthralgia due to wind-dampness in Korean traditional medicine. It is also claimed to be effective in the treatment of acne, erythema, headache, toothache, sinusitis, colds and flu. The present study focused whether the ethyl acetate extract from Angelica Dahuricae Radix (EAAD) inhibits production of nitric oxide (NO), prostaglandin E(2) (PGE(2)) and tumor necrosis factor (TNF)-alpha, as well as expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinases (MAPKs) in lipopolysaccharide (LPS)-stimulated macrophages. EAAD inhibited LPS-induced NO, PGE(2) and TNF-alpha production as well as expression of iNOS and COX-2 in RAW 264.7 cells. EAAD inhibited LPS-induced TNF-alpha production in THP-1 cells. Furthermore, EAAD suppressed LPS-induced phosphorylation of p38 MAPK and extracellular-signal regulated kinases 1/2 (ERK1/2), I-kappaBalpha degradation, and NF-kappaB activation in RAW 264.7 cells. These results suggest that EAAD has the inhibitory effects on LPS-induced TNF-alpha, NO and PGE(2) production, and expression of iNOS and COX-2 in macrophage through blockade in the phosphorylation of MAPKs, following I-kappaBalpha degradation and NF-kappaB activation. PMID:17229575

Kang, Ok-Hwa; Lee, Go-Hoon; Choi, Hyuk Joon; Park, Pil Sang; Chae, Hee-Sung; Jeong, Seung-Il; Kim, Youn-Chul; Sohn, Dong Hwan; Park, Hyun; Lee, John Hwa; Kwon, Dong-Yeul

2007-04-01

169

A Chinese Herbal Decoction, Dang Gui Bu Xue Tang, Prepared from Radix Astragali and Radix Angelicae sinensis, Ameliorates Insulin Resistance Induced by A High-Fructose Diet in Rats  

PubMed Central

Dang Gui Bu Xue Tang (DBT), a Chinese medicinal decoction contains Radix Angelicae sinensis (Danggui) and Radix Astragali (Huangqi) at a ratio of 1?:?5, is used commonly for treating women's ailments. This study was conducted to explore the effects of this preparation on insulin resistance in rats fed with 6-week diet containing 60% fructose. Similar to the action of rosiglitazone (4?mg?kg?1 per day by an oral administration), repeated oral administration of DBT (2.5?g?kg?1 per day) for 14 days was found to significantly alleviate the hyperglycemia but made no influence on plasma lipid profiles nor weight gain in fructose chow-fed rats. Also, the higher degree of insulin resistance as measured by homeostasis model assessment of basal insulin resistance in fructose chow-fed rats was significantly decreased by repeated DBT treatment. DBT displays the characteristic of rosiglitazone by increasing the whole-body insulin sensitivity in fructose chow-fed rats after 2-week treatment, as evidenced by the marked elevation of composite whole-body insulin sensitivity index during the oral glucose tolerance test. DBT improves insulin sensitivity through increased post-receptor insulin signaling mediated by enhancements in insulin receptor substrate-1-associated phosphatidylinositol 3-kinase step and glucose transporter subtype 4 translocation in soleus muscles of animals exhibiting insulin resistance. DBT is therefore proposed as potentially useful adjuvant therapy for patients with insulin resistance and/or the patients who wish to increase insulin sensitivity.

Liu, I-Min; Tzeng, Thing-Fong; Liou, Shorong-Shii

2011-01-01

170

Fo Shou San, an ancient herbal decoction prepared from Angelicae Sinensis Radix and Chuanxiong Rhizoma, induces erythropoietin expression: a signaling mediated by the reduced degradation of hypoxia-inducible factor in cultured liver cells.  

PubMed

Fo Shou San (FSS) is an ancient herbal decoction composed of Angelicae Sinensis Radix (ASR; Danggui) and Chuanxiong Rhizoma (CR; Chuanxiong) in a ratio of 3:2. FSS is mainly prescribed for patients having a deficiency of blood supply, and it indeed has been shown to stimulate the production of erythropoietin (EPO) in cultured cells. In order to reveal the mechanism of this FSS-induced EPO gene expression, the upstream regulatory cascade, via hypoxia-induced signaling, was revealed here in cultured hepatocellular carcinoma cell line Hep3B. The induction of EPO gene expression, triggered by FSS, was revealed in cultured hepatocytes by: (i) the increase of EPO mRNA; and (ii) the activation of the hypoxia response element (HRE), an upstream regulator of the EPO gene. The FSS-induced EPO gene expression was triggered by an increased expression of hypoxia-inducible factor-1 ? (HIF-1 ?) protein; however, the mRNA expression of HIF-1 ? was not altered by the treatment of FSS. The increased HIF-1 ? was a result of reduced protein degradation after the FSS treatment. The current results therefore provide one of the molecular mechanisms of this ancient herbal decoction for its hematopoietic function. PMID:22095261

Bi, Cathy W C; Xu, Li; Zhang, Wendy L; Zhan, Janis Y X; Fu, Qiang; Zheng, Ken Y Z; Chen, Vicky P; Lau, David T W; Choi, Roy C Y; Wang, Tie J; Dong, Tina T X; Tsim, Karl W K

2012-01-01

171

Decursin Isolated from Angelica gigas Nakai Rescues PC12 Cells from Amyloid ?-Protein-Induced Neurotoxicity through Nrf2-Mediated Upregulation of Heme Oxygenase-1: Potential Roles of MAPK  

PubMed Central

Decursin (D), purified from Angelica gigas Nakai, has been proven to exert neuroprotective property. Previous study revealed that D reduced A?25?35-induced cytotoxicity in PC12 cells. Our study explored the underlying mechanisms by which D mediates its therapeutic effects in vitro. Pretreatment of cells with D diminished intracellular generation of ROS in response to A?25?35. Western blot revealed that D significantly increased the expression and activity of HO-1, which was correlated with its protection against A?25?35-induced injury. Addition of ZnPP, an HO-1 competitive inhibitor, significantly attenuated its protective effect in A?25?35-treated cells, indicating the vital role of HO-1 resistance to oxidative injury. Moreover, D induced Nrf2 nuclear translocation, the upstream of HO-1 expression. While investigating the signaling pathways responsible for HO-1 induction, D activated ERK and dephosphorylated p38 in PC12 cells. Addition of U0126, a selective inhibitor of ERK, blocked D-induced Nrf2 activation and HO-1 induction and meanwhile reversed the protection of D against A?25?35-induced cell death. These findings suggest D augments cellular antioxidant defense capacity through both intrinsic free radical scavenging activity and activation of MAPK signal pathways that leads to Nrf2 activation, and subsequently HO-1 induction, thereby protecting the PC12 cells from A?25?35-induced oxidative cytotoxicity.

Li, Li; Du, Ji-kun; Zou, Li-yi; Wu, Tie; Lee, Yong-woo; Kim, Yong-ho

2013-01-01

172

Epigenetic reactivation of Nrf2 in murine prostate cancer TRAMP C1 cells by natural phytochemicals Z-ligustilide and Radix angelica sinensis via promoter CpG demethylation.  

PubMed

Cancer development has been linked to epigenetic modifications of cancer oncogenes and tumor suppressor genes; in advanced metastatic cancers, severe epigenetic modifications are present. We previously demonstrated that the progression of prostate tumors in TRAMP mice is associated with methylation silencing of the Nrf2 promoter and a reduced level of transcription of Nrf2 and Nrf2 target genes. Radix Angelicae Sinensis (RAS; Danggui) is a medicinal herb and health food supplement that has been widely used in Asia for centuries. Z-Ligustilide (Lig) is one of the bioactive components of RAS. We investigated the potential of Lig and RAS to restore Nrf2 gene expression through epigenetic modification in TRAMP C1 cells. Lig and RAS induced the mRNA and protein expression of endogenous Nrf2 and Nrf2 downstream target genes, such as HO-1, NQO1, and UGT1A1. Bisulfite genomic sequencing revealed that Lig and RAS treatment decreased the level of methylation of the first five CpGs of the Nrf2 promoter. A methylation DNA immunoprecipitation assay demonstrated that Lig and RAS significantly decreased the relative amount of methylated DNA in the Nrf2 gene promoter region. Lig and RAS also inhibited DNA methyltransferase activity in vitro. Collectively, these results suggest that Lig and RAS are able to demethylate the Nrf2 promoter CpGs, resulting in the re-expression of Nrf2 and Nrf2 target genes. Epigenetic modifications of genes, including Nrf2, may therefore contribute to the overall health benefits of RAS, including the anticancer effect of RAS and its bioactive component, Lig. PMID:23441843

Su, Zheng-Yuan; Khor, Tin Oo; Shu, Limin; Lee, Jong Hun; Saw, Constance Lay-Lay; Wu, Tien-Yuan; Huang, Ying; Suh, Nanjoo; Yang, Chung S; Conney, Allan H; Wu, Qing; Kong, Ah-Ng Tony

2013-03-18

173

Verification of the formulation and efficacy of Danggui Buxue Tang (a decoction of Radix Astragali and Radix Angelicae Sinensis): an exemplifying systematic approach to revealing the complexity of Chinese herbal medicine formulae  

PubMed Central

This article exemplifies a systematic approach to revealing the complexity of Chinese herbal medicine formulae through three levels of scientific research: standardization of herbs, verification of ancient formulae and mechanism studies. We use Danggui Buxue Tang (DBT) as an example for this approach. Among thousands of traditional Chinese medicine herbal formulae, almost all of which consist of multiple herbs, DBT is one of the simplest. Containing only two herbs, namely Radix Astragali (RA) and Radix Angelicae Sinensis (RAS), DBT is traditionally used to treat ailments in women. The weight ratio of RA to RAS in DBT was prescribed to be 5:1 as early as in 1247 AD. In addition to advanced chemical analysis of herbal constituents, DNA genotyping techniques have been developed for reliable standardization of RA and RAS. Chemical evaluation shows that main active constituents in DBT, including astragaloside IV, calycosin, formononetin and ferulic acid, were most abundant after extraction at the RA to RAS ratio of 5:1, whereas other tested RA to RAS ratios only gave sub-optimal levels of the active constituents. Biological evaluation indicates that bioactivities of DBT, e.g. immuno-modulatory, oesteotropic and estrogenic effects are also best exerted at the RA to RAS ratio of 5:1. Correlation analysis demonstrates statistically significant relationship between the tested chemical constituents and tested bioactivities. Up- and down-regulation of expression of some genes as potential biomarkers has been detected by using gene chip technology. This systematic approach on the basis of herbal standardization, chemical and biological verification and mechanism studies, as exemplified in this article, will be useful to reveal the complexity of not only DBT but also other Chinese medicine herbal formulae.

Gao, Qiutao; Li, Jun; Cheung, Jerry Ka Hei; Duan, Jinao; Ding, Anwei; Cheung, Anna Wing Han; Zhao, Kuijun; Li, Winnie Zhuoming; Dong, Tina Tingxia; Tsim, Karl Wah Keung

2007-01-01

174

High-performance liquid chromatographic method for the determination and pharmacokinetic study of oxypeucedanin hydrate and byak-angelicin after oral administration of Angelica dahurica extracts in mongrel dog plasma.  

PubMed

A high-performance liquid chromatographic method was developed and validated for the determination and pharmacokinetic study of oxypeucedanin hydrate and byak-angelicin after oral administration of Angelica dahurica extracts in mongrel dog plasma. The coumarin components and the internal standard isopsoralen were extracted from plasma samples with the mixture of tert-butyl methyl ether and n-hexane (4:1, v/v). Chromatographic separation was performed on a C(18) column (200 mm x 4.6mm, 5 microm) with the mobile phase acetonitrile-methanol-water-acetic acid (20:15:65:2, v/v/v/v) at a flow-rate of 1.0 ml/min. Only the peak of oxypeucedanin hydrate and byak-angelicin could be detected in dog plasma after oral administration of ethanol extracts of A. dahurica mainly containing xanthotoxol, osthenol, imperatorin, oxypeucedanin hydrate and byak-angelicin. The calibration curves of oxypeucedanin hydrate and byak-angelicin were linear over a range of 22.08-8830.00 and 6.08-2430.00 ng/ml in dog plasma, respectively. The quantification limit of oxypeucedanin hydrate and byak-angelicin in dog plasma was 22.08 and 6.08 ng/ml, respectively. The intra- and inter-day precision was less than 7.6% and 8.5% and the accuracy was from 91.9% to 106.1%. The lowest absolute recoveries of oxypeucedanin hydrate and byak-angelicin were 85.7% and 87.0%, respectively. The method was successfully applied to the pharmacokinetic studies of oxypeucedanin hydrate and byak-angelicin in dog plasma after oral administration of ethanol extracts from A. dahurica. PMID:17344014

Xie, Ying; Chen, Yi; Lin, Mei; Wen, Jun; Fan, Guorong; Wu, Yutian

2007-05-01

175

Safrole-2',3'-oxide induces cytotoxic and genotoxic effects in HepG2 cells and in mice.  

PubMed

Safrole-2',3'-oxide (SAFO) is a reactive electrophilic metabolite of the hepatocarcinogen safrole, the main component of sassafras oil. Safrole occurs naturally in a variety of spices and herbs, including the commonly used Chinese medicine Xi xin (Asari Radix et Rhizoma) and Dong quai (Angelica sinensis). SAFO is the most mutagenic metabolite of safrole tested in the Ames test. However, little or no data are available on the genotoxicity of SAFO in mammalian systems. In this study, we investigated the cytotoxicity and genotoxicity of SAFO in human HepG2 cells and male FVB mice. Using MTT assay, SAFO exhibited a dose- and time-dependent cytotoxic effect in HepG2 cells with TC(50) values of 361.9?M and 193.2?M after 24 and 48h exposure, respectively. In addition, treatment with SAFO at doses of 125?M and higher for 24h in HepG2 cells resulted in a 5.1-79.6-fold increase in mean Comet tail moment by the alkaline Comet assay and a 2.6-7.8-fold increase in the frequency of micronucleated binucleated cells by the cytokinesis-block micronucleus assay. Furthermore, repeated intraperitoneal administration of SAFO (15, 30, 45, and 60mg/kg) to mice every other day for a total of twelve doses caused a significant dose-dependent increase in mean Comet tail moment in peripheral blood leukocytes (13.3-43.4-fold) and in the frequency of micronucleated reticulocytes (1.5-5.8-fold). Repeated administration of SAFO (60mg/kg) to mice caused liver lesions manifested as a rim of ballooning degeneration of hepatocytes immediately surrounding the central vein. Our data clearly demonstrate that SAFO significantly induced cytotoxicity, DNA strand breaks, micronuclei formation both in human cells in vitro and in mice. More studies are needed to explore the role SAFO plays in safrole-induced genotoxicity. PMID:21986196

Chiang, Su-yin; Lee, Pei-yi; Lai, Ming-tsung; Shen, Li-ching; Chung, Wen-sheng; Huang, Hui-fen; Wu, Kuen-yuh; Wu, Hsiu-ching

2011-12-24

176

An Antioxidant Phytotherapy to Rescue Neuronal Oxidative Stress  

PubMed Central

Oxidative stress is involved in the pathogenesis of ischemic neuronal injury. A Chinese herbal formula composed of Poria cocos (Chinese name: Fu Ling), Atractylodes macrocephala (Chinese name: Bai Zhu) and Angelica sinensis (Chinese names: Danggui, Dong quai, Donggui; Korean name: Danggwi) (FBD), has been proved to be beneficial in the treatment of cerebral ischemia/reperfusion (I/R).This study was carried out to evaluate the protective effect of FBD against neuronal oxidative stress in vivo and in vitro. Rat I/R were established by middle cerebral artery occlusion (MCAO) for 1?h, followed by 24?h reperfusion. MCAO led to significant depletion in superoxide dismutase and glutathione and rise in lipid peroxidation (LPO) and nitric oxide in brain. The neurological deficit and brain infarction were also significantly elevated by MCAO as compared with sham-operated group. All the brain oxidative stress and damage were significantly attenuated by 7 days pretreatment with the aqueous extract of FBD (250?mg?kg?1, p.o.). Moreover, cerebrospinal fluid sampled from FBD-pretreated rats protected PC12 cells against oxidative insult induced by 0.2?mM hydrogen peroxide, in a concentration and time-dependent manner (IC50 10.6%, ET50 1.2?h). However, aqueous extract of FBD just slightly scavenged superoxide anion radical generated in xanthine–xanthine oxidase system (IC50 2.4?mg?ml?1) and hydroxyl radical generated in Fenton reaction system (IC50 3.6?mg?ml?1). In conclusion, FBD was a distinct antioxidant phytotherapy to rescue neuronal oxidative stress, through blocking LPO, restoring endogenous antioxidant system, but not scavenging free radicals.

Lin, Zhihong; Zhu, Danni; Yan, Yongqing; Yu, Boyang; Wang, Qiujuan; Shen, Pingniang; Ruan, Kefeng

2011-01-01

177

Managing musical success In a small island environment: Vanessa Quai and the Vanuatu music scene  

Microsoft Academic Search

Vanuatu, formerly known as the New Hebrides (during the period of its Anglo-French colonial condominium) comprises a group of 83 islands in the South West Pacific, north east of New Caledonia and north west of Fiji. Vanuatu is home to a rich Melanesian culture where more than 100 different languages are spoken (in addition to the national pidgin language bislama).

Philip Hayward; Guy Morrow

2007-01-01

178

Displaying colonial artifacts in Paris at the Musée Permanent des Colonies to Musée du Quai Branly  

Microsoft Academic Search

The vast cultural artifacts plundered from Africa, Asia, the Pacific region, and the Americas – regions in which France once had colonies, have been distributed among various museums in Paris. For the past seven decades, much of these artifacts from the colonies were at various museums including the Musée Permanent des Colonies (whose name has changed several times) until President

Fassil Demissie

2009-01-01

179

Menopause: a review of botanical dietary supplements.  

PubMed

Since the release of the Women's Health Initiative (WHI) findings, an increasing number of dietary supplement products specifically targeting women in menopause have appeared in the American marketplace. This growth highlights the need for a critical evaluation of the tolerability and effectiveness of these products. The purpose of this article is to assess the evidence for safety and benefit of botanical monopreparations used for relief of menopause-related symptoms. The Cochrane Library and Medline databases were searched from January 1966 to October 2004, using a detailed list of terms related to botanicals and menopausal symptoms. Studies were considered eligible (1) if they were controlled trials of a botanical monopreparation administered orally for a minimum of 6 weeks to perimenopausal or postmenopausal women with hot flashes and (2) if they included a placebo or comparative treatment arm. Topical preparations, botanical combinations, and dietary interventions, such as soy food or protein, were not included. No language restrictions were imposed on the search. A total of 19 studies met the inclusion criteria. The majority of studies indicate that extract of black cohosh (Actaea racemosa L.) improves menopause-related symptoms; however, methodologic shortcomings in the trials were identified. To date, 4 case reports of possible hepatotoxicity have been published, although previous safety reviews suggest that black cohosh is well tolerated and that adverse events are rare when it is used appropriately. The results of 6 clinical studies on soy (Glycine max L.) isoflavone extracts are mixed. Moreover, the composition and dose of soy supplements varies widely across studies, making comparisons and definitive conclusions difficult. One study challenged the long-term safety of high-dose soy isoflavone extract (150 mg/day for 5 years) on the uterine endometrium. Clinical data from 5 controlled trials assessing the efficacy of semipurified isoflavone red clover (Trifolium pratense L.) leaf extracts to reduce hot flash frequency and severity or to relieve symptoms associated with the domains of the Greene Menopausal Symptom Scale are contradictory. The largest study showed no benefit for reducing symptoms associated with menopause for 2 different red clover isoflavone products compared with placebo. No significant adverse events have been reported in the literature. Single clinical trials do not support the use of dong quai (Angelica sinensis L.), ginseng (Panax ginseng C.A. Mey), or evening primrose seed oil (Oenothera biennis L.) for improving menopausal symptoms. We conclude that black cohosh extracts appear to ease menopausal symptoms; ongoing studies funded by the National Institutes of Health (NIH) will provide more definitive safety and efficacy data. Soy isoflavone extracts appear to have minimal to no effect, although definitive conclusions are difficult given the wide variation in product composition and dose. Long-term safety of higher dosage (150 mg/day) soy isoflavone extracts is uncertain. Semipurified isoflavone red clover leaf extracts have minimal to no effect in reducing menopausal symptoms. Dong quai, ginseng extract, and evening primrose seed oil appear to be ineffective in ameliorating menopausal symptoms at the dosages and in the preparations used in these studies. PMID:16414334

Low Dog, Tieraona

2005-12-19

180

Qiang-Dong proper quantization rule and its applications to exactly solvable quantum systems  

SciTech Connect

We propose proper quantization rule, x{sub A}(x{sub B})k(x)dx-x{sub 0A}(x{sub 0B})k{sub 0}(x)dx=n{pi}, where k(x)={radical}(2M[E-V(x)])/({h_bar}/2{pi}). The x{sub A} and x{sub B} are two turning points determined by E=V(x), and n is the number of the nodes of wave function {psi}(x). We carry out the exact solutions of solvable quantum systems by this rule and find that the energy spectra of solvable systems can be determined only from its ground state energy. The previous complicated and tedious integral calculations involved in exact quantization rule are greatly simplified. The beauty and simplicity of the rule come from its meaning--whenever the number of the nodes of {phi}(x) or the number of the nodes of the wave function {psi}(x) increases by 1, the momentum integral x{sub A}(x{sub B})k(x)dx will increase by {pi}. We apply this proper quantization rule to carry out solvable quantum systems such as the one-dimensional harmonic oscillator, the Morse potential and its generalization, the Hulthen potential, the Scarf II potential, the asymmetric trigonometric Rosen-Morse potential, the Poeschl-Teller type potentials, the Rosen-Morse potential, the Eckart potential, the harmonic oscillator in three dimensions, the hydrogen atom, and the Manning-Rosen potential in D dimensions.

Serrano, F. A. [Instituto Politecnico Nacional, ESIME-Culhuacan Av., Santa Ana 1000, Distrito Federal 04430 (Mexico); Gu Xiaoyan [Department of Physics, East China University of Science and Technology, Shanghai 200237 (China); Dong, Shi-Hai [Escuela Superior de Fisica y Matematicas, Instituto Politecnico Nacional, Edificio 9, Unidad Profesional Adolfo Lopez Mateos, Mexico, Distrito Federal 07738 (Mexico)

2010-08-15

181

Qiang-Dong proper quantization rule and its applications to exactly solvable quantum systems  

NASA Astrophysics Data System (ADS)

We propose proper quantization rule, xAxBk(x)dx-x0Ax0Bk0(x)dx=n?, where k(x)=2M[E-V(x)]/?. The xA and xB are two turning points determined by E=V(x), and n is the number of the nodes of wave function ?(x). We carry out the exact solutions of solvable quantum systems by this rule and find that the energy spectra of solvable systems can be determined only from its ground state energy. The previous complicated and tedious integral calculations involved in exact quantization rule are greatly simplified. The beauty and simplicity of the rule come from its meaning-whenever the number of the nodes of ?(x) or the number of the nodes of the wave function ?(x) increases by 1, the momentum integral xAxBk(x)dx will increase by ?. We apply this proper quantization rule to carry out solvable quantum systems such as the one-dimensional harmonic oscillator, the Morse potential and its generalization, the Hulthén potential, the Scarf II potential, the asymmetric trigonometric Rosen-Morse potential, the Pöschl-Teller type potentials, the Rosen-Morse potential, the Eckart potential, the harmonic oscillator in three dimensions, the hydrogen atom, and the Manning-Rosen potential in D dimensions.

Serrano, F. A.; Gu, Xiao-Yan; Dong, Shi-Hai

2010-08-01

182

How to Continue the Beauty of Traditional Architecture of Dong Village ------the exploration of cultural protection  

NASA Astrophysics Data System (ADS)

Based on architectural culture of ethnic minority and folk customs, keep multiple characteristics of traditional architecture, inject new connotation, meet people's new demand of living condition. Explore a feasible approach to inherit traditional dwellings and diversify architecture. Ensure the healthy development of traditional architecture.

Wen, Fan; Xiaohua, Zhu

183

Effectiveness of lifesaving skills training and improving institutional emergency obstetric care readiness in Lam Dong, Vietnam.  

PubMed

Essential obstetric care is promoted as the prime strategy to save women's lives in developing countries. We measured the effect of improving lifesaving skills (LSS) capacity in Vietnam, a country in which most women deliver in health facilities. A quasi-experimental study was implemented to assess the impact of LSS training and readiness (availability of essential obstetric equipment, supplies, and medication) on the diagnosis of life-threatening obstetric conditions and appropriate management of labor and birth. The intervention (LSS training and readiness) was provided to all clinics and hospitals from 1 of 3 demographically similar districts in southcentral Vietnam, to hospitals only in another district, with the third district serving as the comparison group. Detection of life-threatening obstetric conditions increased in both experimental clinics and hospitals, but the intervention only improved the management of these conditions in hospitals. Management of life-threatening obstetric conditions is most effective in hospitals. The intervention did not clearly benefit women delivering in clinics. PMID:15973269

Sloan, Nancy L; Nguyen, Thi Nhu Ngoc; Do, Trong Hieu; Quimby, Charlotte; Winikoff, Beverly; Fassihian, Goli

2005-01-01

184

Two Objects Not the Same = Liang ge dong xi bu yi yang.  

ERIC Educational Resources Information Center

Studies how Singaporean children differentiate in drawing between a sphere and an elongated, straight-sided ovoid. Tests Piaget's and Inhelder's beliefs that very young children are unable to differentiate in their drawings between differently contoured shapes. Finds that children are able to show the difference in drawings between the two shapes.…

Matthews, John

1997-01-01

185

Governing "Suzhi" and Curriculum Reform in Rural Ethnic China: Viewpoints from the Miao and Dong Communities in Qiandongnan  

ERIC Educational Resources Information Center

This article examines the uptake of "suzhi"--roughly glossed as "quality"--in China's recent curriculum reform called "suzhi jiaoyu" (Education for Quality) in the rural ethnic context of Qiandongnan. It engages with three layers of analysis. First is a brief etymological overview of "suzhi" to map out its cultural politics in contemporary China.…

Wu, Jinting

2012-01-01

186

Mud deposition on the meso-tidal beach due to Typhoon Durian on 4-5th December 2006 in BaDong beach, Vietnam  

NASA Astrophysics Data System (ADS)

BaDnog beach is located in the southeastern margin of the Mekong River Delta, Tra Vinh province, southern Vietnam"CIt is a typical meso-tidal beach system. Since November 2005 we have repeatedly studied surface sedimentation and morphological change controlled by Asian monsoon effects. On March 2007, we described strange mud layers and mud clasts around the high-tide shoreline on Lines A and BT. The diameters of the mud clasts are 1-30 cm and the roundness varies from angular block shape to ellipse spherical shape. Furthermore, we dug among sand bars near the coast and high-tide shoreline. Then we found some mud layers under the beach and sand bars. According to comparison with field data of March 2007 and November 2006, we summarized five important characteristics: (1) Mud layers were found only in the south area such as Lines A and BT because this area had a depositional tendency during March 2007 and November 2006. (2) Mud layers were distributed between the surfaces of March 2007 and November 2006, and mostly spread over the surface of November 2006. (3) The thickness of the mud layers is 1-30 cm. The maximum number of mud layers is three. These are associated with thin sand layers, which consist of very fine sand, derived from beach and associated traction structures such as plane bed and wave ripple. (4) The mud includes much organic materials and its color suggests a subtidal mud origin. The southern Vietnam region has rarely been damaged by strong typhoon since 1997. But, Typhoon Durian hit Vietnam's southern regions on 4-5th December, 2006. We guess the mud layers were brought from the subtidal area to the high-tide shoreline due to the strong waves of this typhoon. Also we guess the mud clasts were washed out from buried mud layers under the beach and sand bars due to the strong waves during the winter monsoon after the typhoon.

Nanayama, F.; Horaguchi, K.; Ta, O. T.; Watanabe, K.; Tateishi, R.; Yamaguchi, T.; Tamura, T.; Nguyen, L. V.; Murakami, F.; Saito, Y.; Tateishi, M.

2008-12-01

187

[Effect of vanadium compounds on the growth and production of coumarins in the suspension culture of Angelica archangelica L].  

PubMed

Plant tissue cultures represent a promising source of substances of natural origin. The main problem of their use is a low production of the majority of secondary metabolites. One of the methods of increasing the production of these substances is elicitation, because the biosynthesis of many secondary metabolites in plant cells is part of the defensive reaction against biological or abiotic stress influences. The paper tested vanadium compounds as potential elicitors of the production of coumarins: sodium vanadate (0.2; 1; 10; 100 and 1000 microM/l of medium) and vanadyl sulfate (10; 20; 50; 100; 200 and 500 microM/l of medium). The toxicity of these substances for the culture was simultaneously monitored by means of the evaluation of the effects on growth (characterized by fresh and dry weights of biomass at the end of two-week cultivation). The cultures were grown both in the light and dark. The growth of the cultures was not influenced by sodium vanadate at concentrations of 0.2 to 100 microM. A vanadate concentration of 1000 microM acted already toxically (in comparison with the control culture, the fresh weight was decreased by 28 % and the dry weight by 41% when cultivated in the light; the fresh weight by 69% and the dry weight by 66% when cultivated in the dark). Vanadyl sulfate in concentrations of 10 to 50 microM did not affect the growth of the culture, at higher concentrations it decreased it gradually: a concentration of 500 microM acted already toxically, again more markedly when cultivated in the light (in comparison with the control culture, the fresh weight was decreased by 27% and dry weight by 38% when cultivated in the light; the fresh weight by 65% and the dry weight by 61% when cultivated in the dark). The production of coumarins was stimulated by sodium vanadate in a concentration of 0.2 and 1 microM when cultivated in the light. The content of coumarins increased in comparison with the control culture mainly in the medium by 46% and by 25% at vanadate concentrations of 0.2 and 1 microM, respectively. Vanadyl sulfate did not increase the production of coumarins. PMID:18064804

Siatka, T; Kasparová, M

2007-10-01

188

Lithium, Sodium, and Potassium Abundances in Sharp-Lined A-Type Stars Takeda, Yoichi; Kang, Dong-Il; Han, Inwoo; Lee, Byeong-Cheol; Kim, Kang-Min; Kawanomoto, Satoshi; Ohishi, Naoko;  

NASA Astrophysics Data System (ADS)

The abundances of alkali elements (Li, Na, and K) were determined from the Li I 6708, Na I 5682/5688, and K I 7699 lines by taking into account the non-LTE effect for 24 sharp-lined A-type stars (ve sin i ? 50 km s-1, 7000 K &lesssim Teff &lessim 10000 K, many showing Am peculiarities to different degrees), based on high-dispersion and high-S/N spectral data secured at BOAO (Korea) and OAO (Japan). We found a significant trend that A(Na) tightly scales with A(Fe) irrespective of Teff, which means that Na becomes enriched similarly to Fe in accordance with the degree of Am peculiarity. Regarding lithium, A(Li) mostly ranges between ˜ 3 and ˜ 3.5 (i.e., almost the same as or slightly less than the solar system abundance of 3.3) with a weak decreasing tendency with a lowering of Teff at Teff &lesssim 8000 K, though several stars exceptionally show distinctly larger depletion. The abundances of potassium also revealed an apparent Teff-dependence in the sense that A(K) in late-A stars tends to be mildly subsolar [possibly with a weak anti-correlation with A(Fe)] systematically decreasing from ˜ 5.0 (Teff ˜ 8500 K) to ˜ 4.6 (Teff ˜ 7500 K), while those for early-A stars remain near-solar around ˜ 5.0-5.2. These observational facts may serve as important constraints for any theory aiming to explain chemical anomalies of A-type stars.

2012-04-01

189

77 FR 14002 - Stainless Steel Butt-Weld Pipe Fittings From Italy, Malaysia, and the Philippines: Final Results...  

Federal Register 2010, 2011, 2012, 2013

...respondent interested party Filmag Italia, srl (Filmag). In its incomplete response...2\\ See Letter to Filmag Italia, srl, from Angelica L. Mendoza, Program Manager...3\\ See Letter to Filmag Italia, srl, from Angelica L. Mendoza, Program...

2012-03-08

190

Decursin from Angelica gigas Nakai induces apoptosis in RC58T\\/h\\/SA#4 primary human prostate cancer cells via a mitochondria-related caspase pathway  

Microsoft Academic Search

Decursin is a major biological active component of Angelicagigas Nakai and is known to induce apoptosis of metastatic prostatic cancer cells. However, the apoptotic mechanism of decursin using primary malignant tumor (RC-58T\\/h\\/SA#4)-derived human prostate cells is not known. In the present study, we show that treatment of prostate cancer cells with decursin inhibited cell proliferation in a dose-dependent manner. Decursin

Sa-Ra Choi; Ju-Hye Lee; Jae-Yong Kim; Kyoung-Wuk Park; Il-Yun Jeong; Ki-Hwan Shim; Mi-Kyung Lee; Kwon-Il Seo

2011-01-01

191

Analysis of the Correlation between Commodity Grade and Quality of Angelica sinensis by Determination of Active Compounds Using Ultraperformance Liquid Chromatography Coupled with Chemometrics  

PubMed Central

The contents of ferulic acid, senkyunolide A, butylidenephthalide, ligustilide, and n-butylphthalide were determined by UPLC analytical method; the correlation among the grade, average weight, and content was explored by correlation analysis and analysis of variance (ANOVA); the different commercial grades with average weight and content were revealed by principal component analysis (PCA) and then rationality analysis grade classification of A. sinensis. The results showed that various commercial grades can be distinguished by PCA analysis. And there was significant negative correlation between the commodity grades and average weight, commodity, and the content of bioactive compounds, while the content of senkyunolide A had significant negative correlation with commodity grades (P < 0.01). Average weight had no correlation with chemicals compounds. Additionally, there was significant positive correlation among the bioactive compounds (content of ferulic acid and phthalides) of different grades of A. sinensis. The content of senkyunolide A, butylidenephthalide, and ligustilide had significant positive correlation with the content of ferulic acid. The content of ligustilide and butylidenephthalide had significant positive correlation with the content of senkyunolide A. The content of ligustilide had significant positive correlation with the content of butylidenephthalide. The basis of grades classification is related with the difference levels of the bioactive compounds.

Wang, Zenghui; Wang, Dongmei; Huang, Linfang

2014-01-01

192

Anti-tumor effects of various furocoumarins isolated from the roots, seeds and fruits of Angelica and Cnidium species under ultraviolet A irradiation.  

PubMed

We examined the effects on cell proliferation of 10 methoxyfurocoumarins and 7 dihydrofurocumarins isolated from Umbelliferae medicinal plants, and their mechanisms of action against B16F10 melanoma cells or in melanin-possessing hairless mice implanted with B16F10 melanoma cells, under UVA irradiation. Furocoumarins having a methoxy group, such as bergapten (1), xanthotoxin (2), phellopterin (4), byakangelicin (6), neobyakangelicin (8), isobergapten (9) and sphondin (10), showed anti-proliferative activity and caused G2/M arrest at concentrations of 0.05-15.0 ?M. The 7 dihydrofurocoumarins had no effect. UVA plus 1, 2, 4, 6 and sec-O-acetylbyakagelicin (7), having one methoxy group at the C-5 position and a linear-type conformation, reduced tumor growth and final tumor weight in B16F10-bearing mice at 0.5 or 1.0 mg/kg (intraperitoneal injection). UVA plus 1 and 2 increased Chk1 phosphorylation and decreased cdc2 (Thr 161) phosphorylation in the melanoma cells. The anti-tumor actions of UVA plus furocoumarins having a methoxy group might be due to the arrest of the cell cycle at G2/M through an increase in phospho-Chk1 and reduction in phospho-cdc2. PMID:23649674

Sumiyoshi, Maho; Sakanaka, Masahiro; Taniguchi, Masahiko; Baba, Kimiye; Kimura, Yoshiyuki

2014-01-01

193

[Effect of manganese (II), cobalt (II), and nickel (II) ions on the growth and production of coumarins in the suspension culture of Angelica archangelica L].  

PubMed

The plant cell reacts to an increased concentration of metals in the environment by various mechanisms. They include an increase in the formation of heat-shock proteins, metallothioneins, phytochelatins, amino acids (cysteine, histidine), organic acids (citric, malic), or secondary metabolites. The latter mechanism is being investigated for its possible use in explant cultures for the stimulation of secondary metabolism, which is the source of substances of pharmaceutical importance. The study tested manganese (II) (0, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, and 50 mM in the medium), cobalt (II), and nickel (II) ions (0, 0.1, 0.5, 1, 5, 10, 50, 100, 200, and 500 microM in the medium) as potential elicitors of coumarin production. At the same time, toxicity of these metals for the culture was examined by evaluating their effect on growth (characterized by fresh and dry weight of biomass at the end of a two-week cultivation). Cultures were cultivated in the dark and in the light. It has been found that the growth of cultures is not influenced by manganese in concentrations ranging from 0 to 2 mM, then it slightly decreases, at a concentration of 50 mM it is lower by 20 % when cultivated in the dark and by 30 % when cultivated in the light in comparison with the control. Cobalt in concentrations of 0 to 50 microM does not significantly influence the growth of the culture, higher concentrations decrease the biomass yields, more markedly when cultivated in the light (at 500 microM Co by 60 %, in the dark only by 30 % in comparison with the controls). Nickel in concentrations of 0.1 to 200 microM does not influence growth, and in a concentration of 500 microM decreases it by approximately 30 % in comparison with the control both in the light and dark. Production of coumarins was not stimulated by any metal in comparison with the control cultures, only the removal of manganese from the medium in the culture cultivated in the dark increased production by about 15 % versus the control. PMID:15751796

Siatka, T; Kasparová, M; Sklenárová, H; Solich, P

2005-01-01

194

Botanical modulation of menopausal symptoms: Mechanisms of action?  

PubMed Central

Menopausal women suffer from a variety of symptoms, including hot flashes and night sweats which can affect quality of life. Although hormone therapy (HT) has been the treatment of choice for relieving these symptoms, HT has been associated with increased breast cancer risk leading many women to search for natural, efficacious, and safe alternatives such as botanical supplements. Data from clinical trials suggesting that botanicals have efficacy for menopausal symptom relief, have been controversial and several mechanisms of action have been proposed including estrogenic, progestogenic, and serotonergic pathways. Plant extracts with potential estrogenic activities include soy, red clover, kudzu, hops, licorice, rhubarb, yam, and chasteberry. Botanicals with reported progestogenic activities are red clover, hops, yam, and chasteberry. Serotonergic mechanisms have also been proposed since women taking antidepressants often report reduction in hot flashes and night sweats. Black cohosh, kudzu, kava, licorice, and dong quai all either have reported 5-HT7 ligands or inhibit serotonin re-uptake, therefore have potential serotonergic activities. Understanding the mechanisms of action of these natural remedies used for women’s health, could lead to more efficacious formulations and to the isolation of active components which have the potential of becoming effective medications in the future.

Hajirahimkhan, Atieh; Dietz, Birgit M.; Bolton, Judy L.

2013-01-01

195

[Microscopic and TLC identification on the fruits of ten species plants for Umbelliferae].  

PubMed

The fruits of ten species plants of Umbelliferae, including the fruits of Peucedanum decursiyum, Saposhnikovia divaricata, Peucedanum terebinthaceun, Anethum graveolens, Cnidium monnieri, Angelica sinensis, Foeniculum vulgate, Angelica polymorpha, Ferula tunnshanica and Cicuta virosa were identified on histology and TLC. PMID:12569827

Peng, Y; Shi, J; Tan, P; Jing, X

1998-10-01

196

Lithuania training needs analysis.  

National Technical Information Service (NTIS)

The present assessment of training needs in the Lithuanian natural gas sector is one of a total of four programmes on which Danish Oil and Natural Gas (DONG) and Lithuanian Gas (LG) cooperate. DONG's contribution is financed by the Danish Foreign Ministry...

1993-01-01

197

Eesti Gaas. Inspection of Kohtal-Jaerve - Tallinn pipeline. Final report.  

National Technical Information Service (NTIS)

The project 'Inspection of Pipelines in Estonia' was funded by Danish government and was carried out in collaboration between DONG, Balslev and FORCE. By agreement between Eesti Gaas and DONG, the pipeline system between Kohtla-Jaerve and Tallinn was sele...

1996-01-01

198

78 FR 8493 - Wooden Bedroom Furniture From the People's Republic of China: Preliminary Results of Antidumping...  

Federal Register 2010, 2011, 2012, 2013

...Alexandre Group, Billy Wood, Huanghekou, and Sheng Jing as part of the PRC-wide entity. As noted above...follows: (1) Billy Wood Industrial (Dong Guan...11\\ The PRC-wide entity includes, among...Furniture Factory; Billy Wood Industrial (Dong...

2013-02-06

199

Herbal medication: potential for adverse interactions with analgesic drugs.  

PubMed

The use of herbal supplements in the US has increased dramatically in recent years. These products are not regulated by the Food and Drug Administration (FDA) with the same scrutiny as conventional drugs. Patients who use herbal supplements often do so in conjunction with conventional drugs. This article is a review of potential adverse interactions between some of the commonly used herbal supplements and analgesic drugs. Non-steroidal anti-inflammatory drugs (NSAIDs), particularly aspirin, have the potential to interact with herbal supplements that are known to possess antiplatelet activity (ginkgo, garlic, ginger, bilberry, dong quai, feverfew, ginseng, turmeric, meadowsweet and willow), with those containing coumarin (chamomile, motherworth, horse chestnut, fenugreek and red clover) and with tamarind, enhancing the risk of bleeding. Acetaminophen may also interact with ginkgo and possibly with at least some of the above herbs to increase the risk of bleeding. Further, the incidences of hepatotoxicity and nephrotoxicity may be augmented by acetaminophen when concomitantly used with the potentially hepatotoxic herbs Echinacea and kava, and with herbs containing salicylate (willow, meadowsweet), respectively. The concomitant use of opioid analgesics with the sedative herbal supplements, valerian, kava and chamomile, may lead to increased central nervous system (CNS) depression. The analgesic effect of opioids may also be inhibited by ginseng. It is suggested that health-care professionals should be more aware of the potential adverse interactions between herbal supplements and analgesic drugs, and take appropriate precautionary measures to avoid their possible occurrences. However, as most of the interaction information available is based on individual case reports, animal studies and in vitro data, further research is needed to confirm and assess the clinical significance of these potential interactions. PMID:12472978

Abebe, W

2002-12-01

200

Determination of fumonisin B1 in botanical roots by liquid chromatography with fluorescence detection: single-laboratory validation.  

PubMed

The accuracy, repeatability, and reproducibility characteristics of a published method for measuring levels of fumonisin B1 (FB1) in botanical root products were determined by an AOAC single-laboratory validation procedure. Replicates of 10 test portions of each powdered root product (black cohosh, echinacea, ginger, ginseng, valerian, dong quai, and turmeric) at each spiking level (FB1 at 0, 50, 100, and 200 ng/g) were analyzed on 3 separate days. Test samples were extracted with methanol-acetonitrile-water (25 + 25 + 100, v/v/v). The extracts were centrifuged, the supernatants diluted with phosphate-buffered saline (PBS) containing 1% Tween 20 and filtered, and the filtrates applied to an immunoaffinity column containing antibodies specific for fumonisins. After the column was washed sequentially with PBS and water, the toxin was eluted from the column with 80% methanol, and the eluate dried by lyophilization. The residue was reconstituted with 50% acetonitrile. FB1 was derivatized with a mixture of o-phthaldialdehyde and mercaptoethanol by using an LC autoinjector. Separations were performed with an RP-LC column, and the FB1 derivative was quantified by fluorescence detection. All root products were found to contain FB1 at <10 ng/g. Average within- and between-day recoveries of FB1 from the botanical roots ranged from 67 to 95% and from 68 to 100%, respectively. Total RSD values for within- and between-day repeatability ranged from 5.5 to 26.4%. HorRat values were <1.3 for all of the matrixes examined. The method meets the AOAC method performance criteria at levels of >50 ng/g for the seven botanical roots tested. PMID:20922947

Oles, Carolyn J; Trucksess, Mary W

2010-01-01

201

76 FR 72164 - Circular Welded Carbon-Quality Steel Pipe From India, the Sultanate of Oman, the United Arab...  

Federal Register 2010, 2011, 2012, 2013

...the Sultanate of Oman, the United Arab Emirates, and the Socialist Republic of Vietnam...Robert James (India, the United Arab Emirates, and Vietnam), or Angelica Mendoza...Sultanate of Oman (Oman), the United Arab Emirates (UAE), and the Socialist...

2011-11-22

202

[The herbalogical textual research on "bai zhi"].  

PubMed

The folowing grounds of "Bai Zhi" (Angelica dahurica) have been researched on the herbalogy, such as medicinal and cultivated history, change of original plant, collecting time, processing method, quality assessement and medicinal part etc. PMID:15376396

Wang, Mengyue; Jia, Minru

2004-05-01

203

Purification of Pharmaceuticals and Nutraceutical Compounds by Sub- and Supercritical Chromatography and Extraction.  

National Technical Information Service (NTIS)

This thesis discusses the use of sub- and supercritical fluids as the medium in extraction and chromatography. Super- and subcritical extraction was used to separate essential oils from herbal plant 'Angelica archangelica'. The effect of extraction parame...

M. Alkio

2008-01-01

204

76 FR 46898 - Quarterly Publication of Individuals, Who Have Chosen To Expatriate, as Required by Section 6039G  

Federal Register 2010, 2011, 2012, 2013

...DELEEDE ANTHONY G DESAI ANAND R DESPERT ALAIN FERNAND DIEMER INGRID H DINGER JR CARL GENE DIPUMA FRANK J DISKO STEFAN C DOMINGO TATIANA SANTO DONG JING ...................... DONOHOE JAMES JPSEPH DONOHUE DAVID LEE DONOHUE...

2011-08-03

205

76 FR 61076 - Initiation of Antidumping and Countervailing Duty Administrative Reviews and Requests for...  

Federal Register 2010, 2011, 2012, 2013

...Food Company, Ltd. QVD Dong Thap Food Co., Ltd. Saigon-Mekong Fishery Co., Ltd. (SAMEFICO) Southern Fishery Industries...Region International Co., Ltd. Richard Hung Ent. Co. Ltd. River Display Ltd. Rizhao Changxing Nail-Making Co., Ltd....

2011-10-03

206

Chemo Might Give Certain Lung Cancer Patients an Edge  

MedlinePLUS

... Dr. Dong-Wan Kim, of the department of internal medicine at Seoul National University Hospital in South Korea. ... M.D., Ph.D., associate professor, department of internal medicine, Seoul National University Hospital, South Korea; Suresh Ramalingam, ...

207

75 FR 9869 - Initiation of Administrative Review of the Antidumping Duty Order on Wooden Bedroom Furniture...  

Federal Register 2010, 2011, 2012, 2013

...Furniture Co., Ltd Sheng Jing Wood Products (Beijing) Co...Dong Guan) Co., Ltd.* World Design International Co...Factory of Yangchun * Yeh Brothers World Trade Inc.* Yuexing Group Co., Ltd. Zhang Zhou Sanlong Wood Product Co., Ltd.*...

2010-03-04

208

76 FR 53409 - Wooden Bedroom Furniture From the People's Republic of China: Corrected Notice of Court Decision...  

Federal Register 2010, 2011, 2012, 2013

...determined for the PRC- wide entity remains unchanged...International Billy Wood Industrial (Dong Guan...Ltd. Sheng Jing Wood Products (Beijing...Ltd. Yeh Brothers World Trade, Inc. Zhang Zhou Sanlong Wood Product Co.,...

2011-08-26

209

78 FR 51209 - Certain Tires and Products Containing Same Notice of Receipt of Complaint; Solicitation of...  

Federal Register 2010, 2011, 2012, 2013

...Number 161 (Tuesday, August 20, 2013)] [Notices] [Pages 51209-51210] [FR Doc No: 2013-20219...Manufacturing Inc. on August 14, 2013. The complaint alleges violations...of China; Doublestar Dong Feng Tyre Co., Ltd. of...

2013-08-20

210

[Research on the mechanism of blood-tonifying effect of danggui buxue decoction].  

PubMed

Danggui Buxue Decoction has shown the effects of significantly promoting the splenic capacity of healthy mice in generating clone-stimulating factors (CSFs) both in spleen conditioned medium (SCM) and in lung conditioned medium (LCM), and markedly enhancing the capacity of the lungs of blood-deficient mice in generating CSFs in spleen conditioned medium. CSFs generation in SCM is promoted in the spleen of healthy mice given Angelica sinensis, but markedly inhibited if Astragulus menbranacous is used. The results suggest that blood-tonifying and blood-activating effects of Danggui Buxue Decoction are associated with its CSFs secretion-stimulating action, which is brought about by Angelica sinensis. PMID:8011126

Chen, Y C; Gao, Y Q

1994-01-01

211

Cryptanalysis and improvement of a DSQC using four-particle entangled state and entanglement swapping  

NASA Astrophysics Data System (ADS)

The security of a deterministic secure quantum communication using four-particle genuine entangled state and entanglement swapping [X.M. Xiu, H.K. Dong, L. Dong, Y.J. Cao, F. Chi, Opt. Commun. 282 (2009) 2457] is analyzed. It is shown that an eavesdropper can entangle an ancilla without introducing any error in the security test utilizing a speciality of the four-particle genuine entangled state. Moreover, the eavesdropper can distill a quarter of the secret information from her entangled ancilla. Finally, a simple improvement to resist this attack is proposed.

Qin, Su-Juan; Wen, Qiao-Yan; Lin, Song; Guo, Fen-Zhuo; Zhu, Fu-Chen

2009-10-01

212

[Phospholipid components of danggui].  

PubMed

This paper deals with the phospholipid components in Danggui (Angelica sinensis and Levisticum officinale) by using thin-layer chromatographic scanning and the corrective method of absorbance proportional coefficient. Seven known and two unknown phospholipid components were separated and determined. The authors have compared the composition of phospholipids for six Danggui samples. PMID:1811671

Guo, R; Lu, J; Xu, Y

1991-12-01

213

"Hay Que Seguir Luchando": Struggles that Shaped English Language Learning of Four Cuban Immigrant Women  

ERIC Educational Resources Information Center

Newly arrived from Cuba, Angelica, Dora, Marina, and Damaris attempted to negotiate new surroundings and immigrant identities, building a sense of home for themselves and their families. Data from qualitative interviews, classroom observations, and focus group conversations revealed hopes that by acquiring English language skills, they would…

Butcher, John S.; Townsend, Jane S.

2011-01-01

214

Comparison of two kinds of magnetic nanoparticles in vivo and in vitro.  

PubMed

This study compared a new type of polysaccharide-coated magnetic nanoparticles (in which the polysaccharide is derived from Angelica sinensis) with the dextran magnetic nanoparticles in terms of preparation, biocompatibility and tissue distribution in vivo and in vitro in order to examine the potential application of Angelica polysaccharide as a novel carrier in magnetic drug targeting (MDT). Magnetic nanoparticles were prepared by chemical co-precipitation. Their physical and chemical properties were determined by using the transmission electron microscope (TEM), laser particle size analyzer (DLS) and vibrating sample magnetometer (VSM), and their purity and structure by using X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The atomic absorption spectrometric method was performed for quantification of the iron content in different tissues. Histological sections were stained by Prussian blue staining to observe the disposition of magnetic nanoparticles in the liver and kidney. The results showed that both kinds of magnetic nanoparticles possessed small particle size, good dispersion and good magnetic properties. XRD showed the main component of the two magnetic nanoparticles was Fe(3)O(4) crystals, and FTIR proved Fe(3)O(4) was successfully coated by each polysaccharide, respectively. In vivo, Fe(3)O(4)-dextran accumulated in the liver, spleen and lung and Fe(3)O(4)-Angelica polysaccharide only in the spleen and lung. It was concluded that Angelica polysaccharide may be applied as a novel carrier in the preparation of magnetic nanoparticles. PMID:22684573

Wang, Liu; Zhang, Yu; Li, Shijun; Wang, Yujuan; Wang, Kaiping

2012-06-01

215

‘Hay que seguir luchando’: struggles that shaped English language learning of four Cuban immigrant women  

Microsoft Academic Search

Newly arrived from Cuba, Angelica, Dora, Marina, and Damaris attempted to negotiate new surroundings and immigrant identities, building a sense of home for themselves and their families. Data from qualitative interviews, classroom observations, and focus group conversations revealed hopes that by acquiring English language skills, they would improve their quality of life in their new country. Struggles included personal factors

John S. Butcher; Jane S. Townsend

2010-01-01

216

‘Hay que seguir luchando’: struggles that shaped English language learning of four Cuban immigrant women  

Microsoft Academic Search

Newly arrived from Cuba, Angelica, Dora, Marina, and Damaris attempted to negotiate new surroundings and immigrant identities, building a sense of home for themselves and their families. Data from qualitative interviews, classroom observations, and focus group conversations revealed hopes that by acquiring English language skills, they would improve their quality of life in their new country. Struggles included personal factors

John S. Butcher; Jane S. Townsend

2011-01-01

217

Monitoring and Modeling of Pavement Response and Performance. Task B: New York. Volume 1: I490, RT9A, and I86 AC Pavement.  

National Technical Information Service (NTIS)

This volume reports on experiments at three pavement sites in New York, I490 in Rochester, RT9A in New York City, and I86 near Angelica. I490 included JPCP sections instrumented to monitor loss of support from curling and warping during curing and early u...

I. S. Khoury J. Morrison S. M. Sargand

2012-01-01

218

Six Week Slavery Novel Unit.  

ERIC Educational Resources Information Center

Developed in conjunction with a graduate course and used in classrooms with all types of learners, this paper presents a 6-week unit of study on slavery based on two adolescent novels--"NIGHTJOHN" by Gary Paulson and "My Name Is not Angelica" by Scott O'Dell. After a brief introduction to the unit, the paper presents the 14 activities of the unit:…

Jones, Darolyn Lyn

219

The anticoagulant ability of ferulic acid and its applications for improving the blood compatibility of silk fibroin  

Microsoft Academic Search

The hemocompatibility of silk fibroin (SF) was improved with ferulic acid (FA) by graft polymerization. Ferulic acid is an active ingredient of many Chinese herbal medicines, such as Chuanxiong (Rhizoma ligustici wallichii), Danggui (Angelica sinensis) and Awei (Asafoetida giantfennel), which have been used to treat cardiovascular diseases by Chinese physicians for thousands of years. The inhibitory functions of FA on

Song Wang; Zhen Gao; Xiaomeng Chen; Xiaojie Lian; Hesun Zhu; Jun Zheng; Lizhong Sun

2008-01-01

220

Milk thistle  

MedlinePLUS

Milk thistle is a plant. The above ground parts and seeds are used to make medicine. The seeds are more commonly used. Milk thistle is ... peppermint leaf, German chamomile, caraway, licorice, clown’s mustard plant, celandine, angelica, and lemon balm. When used daily ...

221

Seven new bifuranocoumarins, dahuribirin A-G, from Japanese Bai Zhi.  

PubMed

Five new spirobifuranocoumarins, dahuribirins A-E (1-5) and two new bifuranocoumarins, dahuribirins F and G (6 and 7) were isolated from Japanese Bai Zhi (the root of Angelica dahurica Benth. et Hook. var. dahurica Benth. et Hook.) and their structures were established by chemical and spectral means. PMID:11558591

Wang, N H; Yoshizaki, K; Baba, K

2001-09-01

222

Revista de Documentacao de Estudos em Linguistica Teorica e Aplicada (DELTA): Novos Estudos em Gamatica Gerativa, 2001 (Journal of Documentary Studies in Theoretical and Applied Linguistics [DELTA]: New Studies in Generative Grammar, 2001).  

ERIC Educational Resources Information Center

These two issues of volume 17, include the following articles: "The Competing Motivation Model in the Functional Domains of Negation" (M. Angelica Furtado da Cunha); "Discursive Resonance and Politeness in Reading and Writing Practices" (Silvana Serrani Infante); "The Acquisition of Relative Clauses in Brazilian Portuguese" (Maria Cecilia…

Barbara, Leila, Ed.; Rajagopalan, Kanavillil, Ed.

2001-01-01

223

Pattern diversity with multi-mode circular patch antennas in clustered MIMO channels  

Microsoft Academic Search

The throughput that a MIMO channel can support is a function of different array parameters. Another technique, for miniaturized antenna designs, is polarization\\/pattern diversity where the antennas are designed to radiate with orthogonal radiation patterns and polarizations as a means to create uncorrelated channels across different array elements. The benefits of pattern diversity have been shown by L. Dong et

Antonio Forenza; Frank Sun; Robert W. Heath Jr

2005-01-01

224

Increase of Farmers' Knowledge through Farmer Seed Production Schools in Vietnam as Assessed on the Basis of Ex-Ante and Ex-Post Tests  

ERIC Educational Resources Information Center

The study was designed to assess changes in farmers' knowledge of farmer seed production through schools (FSPSs) in Vietnam. A set of 25 questions covering five technical areas of the seed production process was used for pre and post knowledge testing at 12 FSPSs in the provinces Binh Dinh, Nam Dinh, Nghe An and Dong Thap. The main findings show…

Tin, Huynh Q.; Struik, Paul C.; Price, Lisa L.; Tuyen, Nguyen P.; Hoan, Nguyen P.; Bos, Heleen

2010-01-01

225

Where does innate immunity stop and adaptive immunity begin?  

PubMed

The regulation of alternative splicing in the immune effector Dscam reported by Dong et al. (2012) in this issue of Cell Host & Microbe raises important questions about the nature of immune responses. Can we clearly define "adaptive" as being different from "innate" immunity, or is it time for a more flexible description? PMID:23084909

Ziauddin, Junaid; Schneider, David S

2012-10-18

226

How to improve the design of the electrical system in future wind power plants  

Microsoft Academic Search

HIS paper presents three topics which are important for better performance of future wind farms. The topics are investigated in three coordinated Ph.D. projects ongoing at the Technical University of Denmark (DTU), Aalborg University (AAU) and DONG Energy. The objective of all projects is to improve the understanding of the main electrical components in wind farms, based on available information,

I. Arana; L. Kocewiak; J. Holbøll; C. L. Bak; A. H. Nielsen; A. Jensen; J. Hjerrild; T. Sørensen

227

A Canon, a Norm and an Attitude--Ideological Dimensions in Foreign Language Education in China.  

ERIC Educational Resources Information Center

An examination of China's policy, "making the ancient serve the present and foreign things serve China," introduced by Mao Ze-dong, is intended to provoke further discussion of the ideological dimension of foreign language education (FLE) in China. Some observers suggest that this has been an important guiding doctrine in FLE since 1949, both…

Feng, Anwei

228

Information Technology in Education and Training (IT@EDU98). Proceedings, Session 1.  

ERIC Educational Resources Information Center

The first session of IT@EDU98 consisted of four papers and was chaired by Dong Thi Bich Thuy (University of Natural Sciences, Ho Chi Minh City, Vietnam). "Technology for Learning: The Present and Future in the United States" (Thomas Owens, Carolyn Cohen) focuses on how technology is changing learning, looks at the most promising opportunities as…

Northwest Regional Educational Lab., Portland, OR.

229

Danish Natural Gas Network. 1980 Status Report and Financial Review.  

National Technical Information Service (NTIS)

Effective August 15, 1978, Dansk Olie og Naturgas A/S (''D.O.N.G. A/S'') retained Kuhn Loeb Lehman Brothers International (''KLLB'') to act as financial advisor to the proposed Danish Natural Gas Transmission and Distribution System (the ''Project''). KLL...

1980-01-01

230

Arsenic and Other Metal Contamination of Groundwater in the Mekong River Delta, Vietnam  

Microsoft Academic Search

High levels of arsenic (As) contamination are found in the groundwater of Vietnam. To determine the distribution of arsenic and other metal contami- nation in the groundwater of the Mekong River Delta, we examined the contamination status of As and other metals in two regions, Tien Giang Province and Dong Thap Province. The concentration of total As in the groundwater,

Yasuhiro Shinkai; Duong Van Truc; Daigo Sumi; Doan Canh; Yoshito Kumagai

2007-01-01

231

New Horizons in Education, 2003.  

ERIC Educational Resources Information Center

This journal, written in English and Chinese, includes the following papers: "Values for Creativity: A Study among Undergraduates in Hong Kong and Guangzhou" (Xia Dong Yue and Kok Leung); "The Present Situation of Family Education at the Turn of the Century: An Investigation in Three South-Eastern Provinces in China" (Feng Luo); "Philosophers…

Ho, Kwok Keung, Ed.

2003-01-01

232

Assessing the use of language learning strategies worldwide with the ESL\\/EFL version of the Strategy Inventory for Language Learning (SILL)  

Microsoft Academic Search

With factor analysis contributions by Neil Anderson, Ohio University, USA; Deena Boraie, American University in Cairo, Egypt; John Green, University of Puerto Rico at Mayaguez and Salem State College, USA; Gene Halleck, Oklahoma State University, USA; Omneya Kassabgy, Career Development Center, Cairo, Egypt; Victoria Talbott, Skagit Valley Junior College, USA; Yoshinori Watanabe, Japan; Nae-Dong Yang, National Taiwan University, Taiwan, ROC;

Rebecca L. Oxford; Judith A. Burry-Stock

1995-01-01

233

English as a Third Language in Rural China: Lessons from the Zaidang Kam-Mandarin Bilingual Education Project  

ERIC Educational Resources Information Center

This article explores the findings of a study that compared 2 groups of 5th-grade first-language Kam-Dong minority students as they learned English as a third language (L3) in the remote mountain village of Zaidang, in Rongjiang county, Guizhou Province, P.R. China. One group had previously been taught using Mandarin only (MO), whereas the other…

Finifrock, Jacob E.

2010-01-01

234

77 FR 59168 - Initiation of Antidumping and Countervailing Duty Administrative Reviews and Request for...  

Federal Register 2010, 2011, 2012, 2013

...Food Company, Ltd. QVD Dong Thap Food Co., Ltd. Saigon-Mekong Fishery Co., Ltd. (also known as SAMEFICO) Seafood Joint...Rich Shipping Company Limited Richard Hung Ent. Co. Ltd. River Display Ltd. Rizhao Changxing Nail-Making Co., Ltd....

2012-09-26

235

78 FR 35249 - Wooden Bedroom Furniture From the People's Republic of China: Final Results of Antidumping Duty...  

Federal Register 2010, 2011, 2012, 2013

...5) Yeh Brothers World Trade, Inc...PRC-wide Entity \\5...5\\ The PRC-wide entity includes...Furniture Factory; Billy Wood Industrial (Dong...Ltd.; Sheng Jing Wood Products (Beijing...will be the PRC-wide rate of 216.01...

2013-06-12

236

Comments on \\  

Microsoft Academic Search

Table of Contents: Herbert Giersch, Gottfried Haberler, Jan Tumlir, Juergen B. Donges, Bela Balassa, Europe's Role in the World Economy; Fritz Machlup, Johann Schollhorn, Norbert Walter Pascal Salin, Roland Vaubel, Roads to Monetary Union; Willi Albers, Fritz Neumark, Carl S. Shoup, Dieter Biehl, Fiscal Harmonization; Dieter Biehl, Bela Balassa, Claus Noe, Sebastian Schnyder, The Scope of a Common Regional Policy;

Herbert Giersch; Gottfried Haberler; Jan Tumlir; Juergen B. Donges; Bela Balassa; Fritz Machlup; Johann Schollhorn; Norbert Walter; Pascal Salin; Roland Vaubel; Willi Albers; Fritz Neumark; Carl S. Shoup; Dieter Biehl; Claus Noe; Sebastian Schnyder; Adolf Weber; Tim Josling; Gerhard Prosi; Gerhard Fels; Klaus Stegemann

1975-01-01

237

A Review on Grain and Nut Deterioration and Design of the Dryers for Safe Storage with Special Reference to Turkish Hazelnuts  

Microsoft Academic Search

Referee: Dr. X. Dong Chen, Department of Chemical and Materials Engineering, The University of Auckland, Private Bag 92019, Auckland New Zealand Turkey produces about 80% of the total hazelnut crop of the world. About 75% of the production are exported. In Turkey hazelnuts are traditionally sun dried, and may be subject to mold growth and subsequent mycotoxin formation due to

Mustafa Özilgen; Murat Özdemir

2001-01-01

238

CUHK Papers in Linguistics, Number 3.  

ERIC Educational Resources Information Center

Papers in this volume include the following: "Constraints on Dative Acquisition by Chinese ESL Learners" (Hua Dong Fan); "The Learnability of Locality Conditions on Quantification" (Thomas Lee); "Do Learning Environments Make a Difference? A Study on the Acquisition of the English Interrogatives by Three Types of Cantonese Classroom Learners"…

Yip, Virginia, Ed.

1991-01-01

239

The Asian Inspiration: Chinese Influences in the Solo Piano Music of Chen Yi  

Microsoft Academic Search

This Treatise is a study of Chen Yi's compositional style, as shaped by her distinctive educational and cultural background. The Treatise covers her most significant solo piano compositions to date, which include Singing in the Mountain, Guessing, Ji-Dong-Nuo, Duo Ye and Ba Ban. Her compositions achieve an impressive fusion of Chinese and Western musical idioms, displaying excellent craftsmanship that is

Chia-Ching Shen

2011-01-01

240

78 FR 78384 - Certain Tires and Products Containing Same; Commission Determination Not to Review an Initial...  

Federal Register 2010, 2011, 2012, 2013

...C. 1337, on September 20, 2013, based on a complaint filed...78 F . 57882 (Sept. 20, 2013). The respondents are Hong...City, China; Doublestar Dong Feng Tyre Co., Ltd. of Shiyan...settlement agreement. On October 24, 2013, complainants Toyo moved...

2013-12-26

241

Are Modern Concepts of Complex Systems Science Useful for Earth Sciences? (Lewis Fry Richardson Medal Lecture)  

NASA Astrophysics Data System (ADS)

The application of methods of complex systems science has a rich tradition in Earth sciences and has enabled substantially new insights into various complex processes there. However, some approaches and findings have been controversially discussed over the last decades. One reason is that they are often basing on strong restrictions and their violation may lead to pitfalls and misinterpretations. Here, we discuss three general concepts of complex systems science: synchronization, recurrence and complex networks and explain that they are indeed useful for better understanding phenomena as recent and past monsoon or El Nino, to detect paleoclimate-variability transitions which are related to human evolution and to identify teleconnections. References Marwan, N., Romano, M., Thiel, M., Kurths, J., Physics Reports 438, 237-329 (2007). Arenas, A., Diaz-Guilera, A., Kurths, J., Moreno, Y., Zhou, C., Physics Reports 469, 93-153 (2008). Marwan, N., Donges, J.F., Zou, Y., Donner, R. and Kurths, J., Phys. Lett. A 373, 4246 (2009). Donges, J.F., Zou, Y., Marwan, N. and Kurths, J. Europhys. Lett. 87, 48007 (2009). Donner, R., Zou, Y., Donges, J.F., Marwan, N. and Kurths, J., Phys. Rev. E 81, 015101(R) (2010). Mokhov, I. I., D. A. Smirnov, P. I. Nakonechny, S. S. Kozlenko, E. P. Seleznev, and J. Kurths, Geophys. Res. Lett. 38, L00F04 (2011). Donges, J., H. Schultz, N. Marwan, Y. Zou, J. Kurths, Eur. J. Phys. B 84, 635-651 (2011). Donges, J., R. Donner, M. Trauth, N. Marwan, H.J. Schellnhuber, and J. Kurths, PNAS 108, 20422-20427 (2011). Malik, N., B. Bookhagen, N. Marwan, and J. Kurths, Climate Dynamics 39, 971 (2012). Runge, J. , J. Heitzig, V. Petoukhov, J. Kurths, Phys. Rev. Lett. 108, 258701 (2012). Menck, P., J. Heitzig, N. Marwan, J. Kurths, Nature Physics (2013).

Kurths, Jürgen

2013-04-01

242

Antioxidant properties of selected Oriental non-culinary\\/nutraceutical herb extracts as evaluated in raw and cooked meat  

Microsoft Academic Search

Ethanol extracts of white peony (WP), red peony (RP), sappanwood (SW), Moutan peony (MP), rehmania (RE) or angelica (AN) were individually added to ground goat meat at 0.5–2.0% (g dry extract\\/100 g final meat sample), and raw and cooked samples were aerobically refrigerated for 0, 3 or 6 days. These extracts and rosemary extract (RO) were also individually added to

J. Han; K. S. Rhee

2005-01-01

243

Direct Catalytic Asymmetric Vinylogous Conjugate Addition of Unsaturated Butyrolactones to ?,?-Unsaturated Thioamides.  

PubMed

Soft Lewis acid/Brønsted base cooperative catalysts have enabled direct catalytic asymmetric vinylogous conjugate addition of ?,?- and ?,?-unsaturated butyrolactones to ?,?-unsaturated thioamides with perfect atom economy. When using ?-angelica lactone and its derivatives as pronucleophiles, as little as 0.5?mol?% catalyst loading was sufficient to complete the reaction necessary to construct consecutive tri- and tetrasubstituted stereogenic centers in a highly diastereo- and enantioselective fashion. PMID:24740836

Yin, Liang; Takada, Hisashi; Lin, Shaoquan; Kumagai, Naoya; Shibasaki, Masakatsu

2014-05-19

244

Inhibition of Trypanosoma cruzi growth by medical plant extracts  

Microsoft Academic Search

This study describes the screening of extracts obtained from 18 plants and two fungi used in the Chinese and Mediterranean traditional medicines on epimastigote forms of Trypanosoma cruzi. The extracts were tested against epimastigote of T. cruzi Bra C15C2 clone in vitro at 27 °C and at a concentration of 250 ?g\\/ml in axenic culture. Angelica dahurica, A. pubescens, A.

G. R Schinella; H. A Tournier; J. M Prieto; J. L R??os; H Buschiazzo; A Zaidenberg

2002-01-01

245

Identification and determination of the major constituents in Traditional Chinese Medicinal formula Danggui-Shaoyao-San by HPLC–DAD–ESI-MS\\/MS  

Microsoft Academic Search

Danggui-Shaoyao-San (DSS), a famous traditional Chinese medicine formula consisting of six herbal medicines (Paeonia lactiflora, Angelica sinensis, Ligusticum chuanxiong, Poria cocos, Atractylodis macrocephalae and Rhizoma Alismatis), has been used as a classical gynecological remedy in China for centuries. However, its active substances have remained unknown. In this paper, an HPLC\\/DAD\\/ESI-MS\\/MS method was developed for the qualitative and quantitative analysis of

Linlin Chen; Jin Qi; Yan-xu Chang; Danni Zhu; Boyang Yu

2009-01-01

246

Protective effects of CVPM on vascular endothelium in rats fed cholesterol diet  

Microsoft Academic Search

Background: The cardiovascular protective mixture (CVPM) is a concoction of nine Chinese traditional medicines: Dan-shen root, Szechwan lovge rhizome, Chinese angelica, Hawthorn fruit, Safflower, Peach seed, Red peony root, earthworm, and membranous milkvetch root. These medicines are used to cure cardiovascular disease in China. Methods: Animal models were established by feeding the Sprague–Dawley (SD) rats with lipid-rich forage. Serum total

Zhigang Tu; Xiaodong Han; Xuying Wang; Yayi Hou; Boyu Shao; Xin Wang; Qizhen Zhou; Qusheng Fan

2003-01-01

247

New anti-inflammatory formulation containing Synurus deltoides extract  

Microsoft Academic Search

Synurus deltoides was previously found to possess significant anti-inflammatory activity especially against chronic inflammation, and strong\\u000a analgesic activityin vivo. In this study, new anti-inflammatory formulation containingS. deltoides extract as a major ingredient was prepared andin vivo activity was evaluated. The plausible action mechanism was also investigated. The new formulation (SAG) contains 1 part ofS. deltoides extract, 0.9 part ofAngelica gigas

Yong Hwan Choi; Kun Ho Son; Hyeun Wook Chang; KiHwan Bae; Sam Sik Kang; Hyun Pyo Kim

2005-01-01

248

Imperatorin sustained-release tablets: In Vitro and pharmacokinetic studies  

Microsoft Academic Search

We prepared and evaluated imperatorin (IMP) sustained-release tablets. IMP is an active compound in Angelica dahuricae, a\\u000a Chinese herbal medicine. We used different polymers, such as hydroxypropyl methylcellulose (HPMC K4M, K15M, and K100M), carbopol\\u000a 934P, sodium carboxymethyl cellulose (CMC-Na), and their combinations to prepare the matrix tablets and achieve the desired\\u000a sustained release profile. The in vitro release profiles of

Jingjing Pan; Wen Lu; Changhui Li; Sicen Wang; Langchong He

2010-01-01

249

Science Signaling Podcast: 14 May 2013  

NSDL National Science Digital Library

This Podcast features an interview with Chen Dong, senior author of a Research Article that appears in the 14 May 2013 issue of Science Signaling. A group led by Dong found that the deubiquitinase USP25 modulates inflammatory signaling initiated by Toll-like receptor 4 (TLR4). Activation of TLR4 by the bacterial cell wall component lipopolysaccharide (LPS) activates two signaling pathways that promote the production of various cytokines that control inflammation. Zhong et al. report that USP25 affected signaling through one of these pathways to prevent excessive inflammation, which can damage tissues. Compared to wild-type controls, mice lacking USP25 exhibited an excessive inflammatory response and were more susceptible to septic shock following LPS treatment. Understanding the mechanisms that ensure balance in the inflammatory response is important for developing strategies to treat diseases that result from excessive inflammation.

Chen Dong (Houston;MD Anderson Cancer Center REV); Annalisa M. VanHook (American Association for the Advancement of Science;Science Signaling REV)

2013-05-14

250

Irritant contact dermatitis risk of common topical traditional chinese medicines used for skin-lightening: a pilot clinical trial with 30 volunteers.  

PubMed

Topical traditional Chinese medicine- (TTCM-) related contact dermatitis is not uncommon but ignored. Patch and photopatch tests using 6 individual herbal ingredients and Bai-Zhi-Kao (BZK; ), a skin-lightening TTCM preparation, were conducted on 30 participants. Twenty-five subjects showed at least 1 positive reaction, including 6 (20.0%) participants who reacted to BZK. The majority reacted to Radix Ampelopsis japonica (Bai-Lian; ) (60.0%), whereas few reacted to Rhizoma Bletilla striata (Bai-Ji; ) (16.7%), Rhizoma Atractylodis macrocephalae (Bai-Zhu; ) (10.0%), Radix Angelicae dahuricae (Bai-Zhi; ) (3.3%), and Herba asari (Xi-Xin; ) (3.3%). In the photopatch test, 3 participants (10.0%) reacted positively to BZK and 10 to ?1 constituent; however, all reacted to Radix Angelicae dahuricae (26.7%), Radix Ampelopsis japonica (13.3%), and Rhizoma Bletilla striata (3.3%). In contrast, no subjects showed positive reactions to Sclerotium Poria cocos (Bai-Fu-Ling; ). Thus, BZK and its constituents might present potential latent risk of contact dermatitis owing to the possible presence of Radix Ampelopsis japonica and Radix Angelicae dahuricae. Furthermore, TTCMs, particularly cosmetic products, must be used carefully, with ample warning of potential contact dermatitis risk. PMID:24817899

Tsai, Kao-Sung; Lin, Tzu-Chun; Wu, Meng-Tse; Shen, Jui-Lung; Mao, Ming-Ya; Chen, Huey-Yi; Chen, Yung-Hsiang; Chen, Wen-Chi

2014-01-01

251

Irritant Contact Dermatitis Risk of Common Topical Traditional Chinese Medicines Used for Skin-Lightening: A Pilot Clinical Trial with 30 Volunteers  

PubMed Central

Topical traditional Chinese medicine- (TTCM-) related contact dermatitis is not uncommon but ignored. Patch and photopatch tests using 6 individual herbal ingredients and Bai-Zhi-Kao (BZK; ???), a skin-lightening TTCM preparation, were conducted on 30 participants. Twenty-five subjects showed at least 1 positive reaction, including 6 (20.0%) participants who reacted to BZK. The majority reacted to Radix Ampelopsis japonica (Bai-Lian; ??) (60.0%), whereas few reacted to Rhizoma Bletilla striata (Bai-Ji; ??) (16.7%), Rhizoma Atractylodis macrocephalae (Bai-Zhu; ??) (10.0%), Radix Angelicae dahuricae (Bai-Zhi; ??) (3.3%), and Herba asari (Xi-Xin; ??) (3.3%). In the photopatch test, 3 participants (10.0%) reacted positively to BZK and 10 to ?1 constituent; however, all reacted to Radix Angelicae dahuricae (26.7%), Radix Ampelopsis japonica (13.3%), and Rhizoma Bletilla striata (3.3%). In contrast, no subjects showed positive reactions to Sclerotium Poria cocos (Bai-Fu-Ling; ???). Thus, BZK and its constituents might present potential latent risk of contact dermatitis owing to the possible presence of Radix Ampelopsis japonica and Radix Angelicae dahuricae. Furthermore, TTCMs, particularly cosmetic products, must be used carefully, with ample warning of potential contact dermatitis risk.

Tsai, Kao-Sung; Lin, Tzu-Chun; Wu, Meng-Tse; Shen, Jui-Lung; Mao, Ming-Ya; Chen, Huey-Yi; Chen, Wen-Chi

2014-01-01

252

Macromol. Rapid commun. 11/2014.  

PubMed

Front Cover: A facile method is developed for entrapment of proteins into porous poly(D,L-lactide-co-glycolide) (PLGA) microspheres to achieve sustained delivery of the proteins. The PLGA microspheres with large inner pores and closed surface pores can release proteins without denaturation for a period of over two months. Further information can be found in the article by Dong-Hyun Paik and Sung-Wook Choi* on page 1033. PMID:24889011

Paik, Dong-Hyun; Choi, Sung-Wook

2014-06-01

253

The slope seismic response monitoring of Wenchuan aftershocks in Qingchuan  

NASA Astrophysics Data System (ADS)

This work reports some new progress of rock slope inside seismic response monitoring results in the area of Mountain Dong and Mountain Shizi (Qingchuan county), located more than 250 km NE of Yingxiu epicenter (2008 Wenchuan earthquake), Sichuan province. Five adits with the maximum depth of 15 m had been excavated in different elevation on both sides slope. Stations were emplaced at middle of the adits, from September 2009 to May 2010 more than 60 Wenchuan aftershocks had been monitored, 22 typical aftershocks had been analysis, whose magnitude varied between 2.3 ~ 5.2 and epicentral distance was from a few to 45 km. A comparison analysis of recordings provided evidence of the presence amplification effect at the Q4 station of Mt. Dong, which the peak horizontal acceleration amplification factor is between 1.0 ~ 2.5. But this amplification effect had no stronger at other stations. Comprehensive studies show that the relative height to riverbed is an important factor of Q4 seismic amplification effect. Otherwise the topography of Q4 site is conducive to horizontal amplification, not the vertical amplification. Moreover the calculation of Arias intensity (Ia) had the same amplification effect as the PGA, only the amplification factor is between 1.0 ~ 3.47 much bigger than the latter. On the other hand, the calculation of horizontal to vertical spectral ratio (HVSR) at Q4 shows the curves have multiple peaks corresponding with different dominant frequencies, which the amplification factor is always bigger than other stations at Mt. Dong. Sufficient evidence indicates that the Mt. Dong amplification effect is stronger than Mt. Shizi.

Luo, Y. H.; Huang, R.; Wang, Y.

2014-06-01

254

In situ monitoring of animal micronuclei before the operation of Daya Bay Nuclear Power Station  

Microsoft Academic Search

Daya Bay Nuclear Power Station, a newly-built nuclear power station in southern mainland China, started its operation in 1993. We examined micro-nucleated cells of Invertibrate (Bivalves) and Vertibrate (Fish and Amphibia) in different spots within the 50km surroundings of the Power Station during 1986-1993. This paper reports the results of the investigation carried out in Dong Shan, a place 4.7km

Y. N. Cai; H. Y. He; L. M. Qian; G. C. Sun; J. Y. Zhao

1994-01-01

255

THERMOELASTICITY OF CIRCULAR CYLINDRICAL SHELLS LAMINATED OF BIMODULUS COMPOSITE MATERIALS  

Microsoft Academic Search

Closed-form and finite-element solutions are presented for the thermoelastic behavior of laminated composite shells. The material of each layer is assumed to be thermoelastically orthotropic and bimodular, that is, having different properties depending upon whether the fiber-direction normal strain is tensile or compressive. The formulations are based on the thermoelastic generalization of Dong and Tso 's laminated shell theory, which

Y. S. Hsu; J. N. Reddy; C. W. Bert

1981-01-01

256

On regularity criterion for the dissipative quasi-geostrophic equations  

NASA Astrophysics Data System (ADS)

In this paper we consider the 2D dissipative quasi-geostrophic equations and study the regularity criterion of the solutions. By means of a commutator estimate based on frequency localization and Bony's paraproduct decomposition, we obtain a regularity criterion which is an obvious extension of the result in [B. Dong, Z. Chen, A remark on regularity criterion for the dissipative quasi-geostrophic equations, J. Math. Anal. Appl. 329 (2007) 1212-1217] where their restrictive condition on p is .

Yuan, Jia

2008-04-01

257

Tectonics, granitoids and mesozoic gold deposits in East Shandong, China  

Microsoft Academic Search

Mesozoic granitoids in East Shandong1East Shandong gold province is also called Jiaodong gold province, where “Jiao” refers to the Jiaozhou Peninsula, and “dong” stands for east.1 contain some 30 Moz gold, making East Shandong the largest granitoid-hosted gold province in the world. The gold province is located along the northern boundary of the suture zone between the North China and

Taihe Zhou; Guxian Lü

2000-01-01

258

The oribatid mite genus Papillocepheus (Acari, Oribatida, Tetracondylidae), with description of a new species from southern Vietnam.  

PubMed

The genus Papillocepheus is recorded in the Oriental region for the first time. A new species, Papillocepheus primus sp. n., is described from southern Vietnam; the description is based on specimens collected from semidecayed leaves and litter of Dong Nai Biosphere Reserve and Bu Gia Map National Park. The new generic diagnosis of Papillocepheus and an identification key to the known species of this genus are given. PMID:24624013

Ermilov, Sergey G; Anichkin, Alexander E; Tolstikov, Andrei V

2014-01-01

259

2006 Bibliography — CBIIT: Welcome to the NCI Center for Biomedical Informatics and Information Technology  

Cancer.gov

Bakay M, Wang Z, Melcon G, Schiltz L, Xuan J, Zhao P, Sartorelli V, Seo J, Pegoraro E, Angelini C, Shneiderman B, Escolar D, Chen YW, Winokur ST, Pachman LM, Fan C, Mandler R, Nevo Y, Gordon E, Zhu Y, Dong Y, Wang Y, Hoffman EP. Nuclear envelope dystrophies show a transcriptional fingerprint suggesting disruption of Rb-MyoD pathways in muscle regeneration. Brain.

260

Erosion and Nutrient Loss on Sloping Land under Intense Cultivation in Southern Vietnam  

Microsoft Academic Search

To help improve the well-being of the local people, a joint Vietnamese-UK team set out to establish a way of estimating soil and nutrient losses under different land management scenarios, using field data extrapolated through remote sensing and GIS, to obtain catchment-wide estimates of the impact of land cover change. Immigration from remote provinces to the Dong Phu District of

NGUYEN VAN DE; IAN DOUGLAS; JULIA MCMORROW; SARAH LINDLEY; DAO KIM NGUYEN THUY BINH; TRAN THI VAN; LE HUU THANH; NGUYEN THO

2008-01-01

261

English as a Third Language in Rural China: Lessons From the Zaidang Kam–Mandarin Bilingual Education Project  

Microsoft Academic Search

This article explores the findings of a study that compared 2 groups of 5th-grade first-language Kam–Dong minority students as they learned English as a third language (L3) in the remote mountain village of Zaidang, in Rongjiang county, Guizhou Province, P.R. China. One group had previously been taught using Mandarin only (MO), whereas the other had received additive bilingual intervention in

Jacob E. Finifrock

2010-01-01

262

Nonlinear Time Series Analysis in Earth Sciences - Potentials and Pitfalls  

NASA Astrophysics Data System (ADS)

The application of methods of nonlinear time series analysis has a rich tradition in Earth sciences and has enabled substantially new insights into various complex processes there. However, some approaches and findings have been controversially discussed over the last decades. One reason is that they are often bases on strong restrictions and their violation may lead to pitfalls and misinterpretations. Here, we discuss three general concepts of nonlinear dynamics and statistical physics, synchronization, recurrence and complex networks and explain how to use them for data analysis. We show that the corresponding methods can be applied even to rather short and non-stationary data which are typical in Earth sciences. References Marwan, N., Romano, M., Thiel, M., Kurths, J.: Recurrence plots for the analysis of complex systems, Physics Reports 438, 237-329 (2007) Arenas, A., Diaz-Guilera, A., Kurths, J., Moreno, Y., Zhou, C.: Synchronization in complex networks, Physics Reports 469, 93-153 (2008) Marwan, N., Donges, J.F., Zou, Y., Donner, R. and Kurths, J., Phys. Lett. A 373, 4246 (2009) Donges, J.F., Zou, Y., Marwan, N. and Kurths, J. Europhys. Lett. 87, 48007 (2009) Donner, R., Zou, Y., Donges, J.F., Marwan, N. and Kurths, J., Phys. Rev. E 81, 015101(R) (2010)

Kurths, Jürgen; Donges, Jonathan F.; Donner, Reik V.; Marwan, Norbert; Zou, Yong

2010-05-01

263

Simultaneous analysis of nine components in patch preparations of Ru-Yi-Jin-Huang-San by high-performance liquid chromatography  

Microsoft Academic Search

A facile high-performance liquid chromatography (HPLC) method for the resolution and quantitative measurement of nine marker\\u000a substances, the active ingredients in patch preparations of Ru-Yi-Jin-Huang-San, was established using gradient elution in\\u000a the reversed-phase mode. These marker substances included berberine (Phellodendri Cortex), curcumin (Curcumae Rhizoma), imperatorin\\u000a (Angelicae Dahuricae Radix), magnolol (Magnoliae Cortex), hesperidin (Citri Leiocarpae Exocarpium), glycyrrhizin (Glycyrrhizae\\u000a Radix), and emodin,

Horng-Liang Lay; Chia-Chi Chen; Shiow-Chyn Huang; Thau-Ming Cham; Tian-Shung Wu; I-Hsin Lin

2010-01-01

264

Susceptibilities of Mycoplasma hominis to herbs.  

PubMed

To determine the susceptibilities of Mycoplasma homonis (M. hominis) to Chinese medicinal herbs in vitro, 30 clinical strains of M. hominis were isolated and identified from the clinical specimen. The susceptibilities of M. hominis to 19 herbs were determined by serial dilution methods in vitro. The results showed that M. hominis was susceptible to Radix Isatidis, Radix Angelicae Dahuricae, Cortex Phellodendri, Radix et Rhizoma Rhei, Fructus Kochiae and Herba Houttuyniae. These findings laid a foundation in treating M. hominis infection with Chinese herbs. PMID:15974478

Che, Ya-Min; Mao, Shu-He; Jiao, Wen-Ling; Fu, Zhi-Yi

2005-01-01

265

[Research progress studies on pharmacology and pharmacokinetics of ligustilide].  

PubMed

Ligustilide is contained highly or around 1% in such umbelliferous plants as Angelica sinensis and Ligusticum chuanxiong, is one of main bioactive constituents. It shows many pharmacological activities related to their efficacy. At present, ligustilide has attracted extensive attention and more and more studies have been reported, indicating that it is a promising compound. This essay summarizes the progress of pharmacological effects of ligustilide on neuroprotection, vasodilatation, anti-caner and anti-tumor, analgesia and anti-inflammation, and pharmacokinetics including absorption, distribution, metabolism and excretion, providing basis for further studies and development of ligustilide. PMID:23373200

Zuo, Ai-Hua; Wang, Li; Xiao, Hong-Bin

2012-11-01

266

[Analysis and identification of chemical constituents in Siwu decoction by UPLC-Q-TOF-MS(E)].  

PubMed

This research analyzed the chemical constituents of Siwu decoction by UPLC-Q-TOF-MS(E). Base on the data of mass and related-literatures, 43 peaks were profiled and 25 compounds, which contain 8 monoterpene glycosides from Paeonia lactiflora and 13 phthalides from Rhizoma chuanxiong and Radix angelica sinensis mainly, were identified in both positive and negative mode respectively. Meanwhile, chemical constituents of water extract and 60% ethanol extract of Siwu decoction were compared by the principal constituent analysis with MarkerLynx software, which provides the basis for the active ingredients of Siwu decoction. PMID:24494558

Wang, Zhen-Fang; Zhao, Yang; Pang, Xu; Yu, He-Shui; Kang, Li-Ping; Gao, Yue; Ma, Bai-Ping

2013-11-01

267

An update on antitumor activity of naturally occurring chalcones.  

PubMed

Chalcones, which have characteristic 1,3-diaryl-2-propen-1-one skeleton, are mainly produced in roots, rhizomes, heartwood, leaves, and seeds of genera Angelica, Sophora, Glycyrrhiza, Humulus, Scutellaria, Parartocarpus, Ficus, Dorstenia, Morus, Artocarpus, and so forth. They have become of interest in the research and development of natural antitumor agents over the past decades due to their broad range of mechanisms including anti-initiation, induction of apoptosis, antiproliferation, antimetastasis, antiangiogenesis, and so forth. This review summarizes the studies on the antitumor activity of naturally occurring chalcones and their underlying mechanisms in detail during the past decades. PMID:23690855

Zhang, En-Hui; Wang, Ru-Feng; Guo, Shu-Zhen; Liu, Bin

2013-01-01

268

Antiproliferative constituents in Umbelliferae plants II. Screening for polyacetylenes in some Umbelliferae plants, and isolation of panaxynol and falcarindiol from the root of Heracleum moellendorffii.  

PubMed

Methanol extracts of 36 samples of 21 Umbelliferae plants were screened for polyacetylenic compounds using the ELISA for panaxytriol, and their antiproliferative activity was checked by MTT assay using the tumor cell lines MK-1, HeLa and B16F10. The presence of antiproliferative polyacetylenes was suggested in Angelica acutiloba (fruit), Anethum graveolens (root), Bupleurum rotundifolium (fruit), Carum carvi (fruit and root), Coriandrum sativum (fruit), Cryptotaenia japonica (leaf), Glehnia littoralis (fruit), Heracleum moellendorffii (root) and Torilis japonica (fruit). Panaxynol and falcarindiol were successfully isolated from the root of Heracleum moellendorffii as antiproliferative polyacetylenes. PMID:9556156

Nakano, Y; Matsunaga, H; Saita, T; Mori, M; Katano, M; Okabe, H

1998-03-01

269

[Trading of crude drugs between China and Russia as recorded in 3 Chinese versions of Russian works].  

PubMed

Trading of crude drugs between China and Russia appeared before the 19(th) century. By then, exports of Chinese crude drugs to Russia were mainly plants, with Radix et Rhizoma Rhei as the most exported one, followed by both dietary and medicinal aromatic plants, such as fennel, cinnamon bark, pepper, ginger, and Radix Angelica sinensis, and quinine. Imports of Russian drugs were mainly of animal ones, especially those valuable animals like musk sac. In 1854, the importation of a large amount of such sac, numbering as many as 70 000, was very remarkable. Other drugs included stag antler, tender horn of deer, demonstrating typically the regional and productive characteristics of Russia. PMID:24774893

Ding, Zhaoping; Wang, Zhenguo

2014-01-01

270

Network of Networks and the Climate System  

NASA Astrophysics Data System (ADS)

Network of networks is a new direction in complex systems science. One can find such networks in various fields, such as infrastructure (power grids etc.), human brain or Earth system. Basic properties and new characteristics, such as cross-degree, or cross-betweenness will be discussed. This allows us to quantify the structural role of single vertices or whole sub-networks with respect to the interaction of a pair of subnetworks on local, mesoscopic, and global topological scales. Next, we consider an inverse problem: Is there a backbone-like structure underlying the climate system? For this we propose a method to reconstruct and analyze a complex network from data generated by a spatio-temporal dynamical system. This technique is then applied to 3-dimensional data of the climate system. We interpret different heights in the atmosphere as different networks and the whole as a network of networks. This approach enables us to uncover relations to global circulation patterns in oceans and atmosphere. The global scale view on climate networks offers promising new perspectives for detecting dynamical structures based on nonlinear physical processes in the climate system. This concept is applied to Indian Monsoon data in order to characterize the regional occurrence of strong rain events and its impact on predictability. References: Arenas, A., A. Diaz-Guilera, J. Kurths, Y. Moreno, and C. Zhou, Phys. Reports 2008, 469, 93. Donges, J., Y. Zou, N. Marwan, and J. Kurths, Europhys. Lett. 2009, 87, 48007. Donner, R., Y. Zou, J. Donges, N. Marwan, and J. Kurths, Phys. Rev. E 2010, 81, 015101(R ). Mokhov, I. I., D. A. Smirnov, P. I. Nakonechny, S. S. Kozlenko, E. P. Seleznev, and J. Kurths, Geophys. Res. Lett. 2011, 38, L00F04. Malik, N., B. Bookhagen, N. Marwan, and J. Kurths, Climate Dynamics, 2012, 39, 971. Donges, J., H. Schultz, N. Marwan, Y. Zou, J. Kurths, Eur. J. Phys. B 2011, 84, 635-651. Donges, J., R. Donner, M. Trauth, N. Marwan, H.J. Schellnhuber, and J. Kurths, PNAS 2011, 108, 20422-20427. Runge, J. , J. Heitzig, V. Petoukhov, J. Kurths, Phys. Rev. Lett. 2012, 108, 258701.

Kurths, Jürgen; Boers, Niklas; Bookhagen, Bodo; Donges, Jonathan; Donner, Reik; Malik, Nishant; Marwan, Norbert; Stolbova, Veronika

2013-04-01

271

Assessing genetic diversity of Vietnamese local chicken breeds using microsatellites.  

PubMed

This study aimed to assess genetic diversity within and between nine Vietnamese local chicken breeds and two Chinese breeds included for comparison. Genotyping 29 microsatellites revealed high diversity of both Vietnamese and Chinese breeds. Cluster analysis using the STRUCTURE software suggested six clusters as the most likely grouping of the 11 breeds studied. These groups encompassed four homogeneous clusters, one formed by the two Chinese breeds and the other three representing a single breed each: the Mekong Delta breed Ac, the South Central Coast breed Choi, and the Red River Delta breed Dong Tao. The six remaining breeds formed two additional admixed clusters. PMID:20394606

Cuc, N T K; Simianer, H; Eding, H; Tieu, H V; Cuong, V C; Wollny, C B A; Groeneveld, L F; Weigend, S

2010-10-01

272

STRUCTURAL BIOLOGY: Flipping Lipids: Is the Third Time the Charm?  

NSDL National Science Digital Library

Access to the article is free, however registration and sign-in are required. Certain membrane-spanning proteins expel hydrophobic molecules such as lipids and drugs from cells, but just how they accomplish this has been unclear. As Davidson and Chen describe in their Perspective, two new studies (Dong et al., and Reyes et al.) explain how a transporter protein called MsbA expends energy and changes its organization to flip a molecule from one side of the membrane to the other.

Amy L. Davidson (Baylor College of Medicine;Department of Molecular Virology and Microbiology); Jue Chen (Purdue University;Department of Biological Sciences)

2005-05-13

273

Unacceptably High Mortality Related to Measles Epidemics in Niger, Nigeria, and Chad  

PubMed Central

Background Despite the comprehensive World Health Organization (WHO)/United Nations Children's Fund (UNICEF) measles mortality–reduction strategy and the Measles Initiative, a partnership of international organizations supporting measles mortality reduction in Africa, certain high-burden countries continue to face recurrent epidemics. To our knowledge, few recent studies have documented measles mortality in sub-Saharan Africa. The objective of our study was to investigate measles mortality in three recent epidemics in Niamey (Niger), N'Djamena (Chad), and Adamawa State (Nigeria). Methods and Findings We conducted three exhaustive household retrospective mortality surveys in one neighbourhood of each of the three affected areas: Boukoki, Niamey, Niger (April 2004, n = 26,795); Moursal, N'Djamena, Chad (June 2005, n = 21,812); and Dong District, Adamawa State, Nigeria (April 2005, n = 16,249), where n is the total surveyed population in each of the respective areas. Study populations included all persons resident for at least 2 wk prior to the study, a duration encompassing the measles incubation period. Heads of households provided information on measles cases, clinical outcomes up to 30 d after rash onset, and health-seeking behaviour during the epidemic. Measles cases and deaths were ascertained using standard WHO surveillance-case definitions. Our main outcome measures were measles attack rates (ARs) and case fatality ratios (CFRs) by age group, and descriptions of measles complications and health-seeking behaviour. Measles ARs were the highest in children under 5 y old (under 5 y): 17.1% in Boukoki, 17.2% in Moursal, and 24.3% in Dong District. CFRs in under 5-y-olds were 4.6%, 4.0%, and 10.8% in Boukoki, Moursal, and Dong District, respectively. In all sites, more than half of measles cases in children aged under 5 y experienced acute respiratory infection and/or diarrhoea in the 30 d following rash onset. Of measles cases, it was reported that 85.7% (979/1,142) of patients visited a health-care facility within 30 d after rash onset in Boukoki, 73.5% (519/706) in Moursal, and 52.8% (603/1,142) in Dong District. Conclusions Children in these countries still face unacceptably high mortality from a completely preventable disease. While the successes of measles mortality–reduction strategies and progress observed in measles control in other countries of the region are laudable and evident, they should not overshadow the need for intensive efforts in countries that have just begun implementation of the WHO/UNICEF comprehensive strategy.

Grais, R. F; Dubray, C; Gerstl, S; Guthmann, J. P; Djibo, A; Nargaye, K. D; Coker, J; Alberti, K. P; Cochet, A; Ihekweazu, C; Nathan, N; Payne, L; Porten, K; Sauvageot, D; Schimmer, B; Fermon, F; Burny, M. E; Hersh, B. S; Guerin, P. J

2007-01-01

274

Numerical Simulation of Petroleum Generation and Migration in the Song Hong Basin, Vietnam  

NASA Astrophysics Data System (ADS)

The numerical modeling of petroleum systems is an effective tool to understand generation, migration and accumulation of hydrocarbons in a sedimentary basin and hence to determine future targets for the hydrocarbon exploration. The numerical modeling identifies two petroleum systems in the Song Hong Basin, which is a petroliferous Cenozoic basin, offshore eastern Vietnam. These petroleum systems were named DinhCao-PhuCu(.) Petroleum System and SongHuong-BienDong(.) Petroleum System. DinhCao-PhuCu(.) Petroleum System covers northern and central parts of the Song Hong basin with Oligocene shale and coaly shale source rocks of Dinh Cao formation, which are dominated by type II-III kerogens. The hydrocarbon generation starts at 13 Ma within deeply buried Oligocene strata located in the centre of the basin. The hydrocarbon expels from the Oligocene source rock and migrates laterally and then up dip toward marginal areas where Middle Miocene sandstones of Phu Cu formation are present as major reservoirs. The numerical model shows that the critical moment occurs at about 3.5 Ma. The DinhCao-PhuCu(.) petroleum system is confirmed by sparse occurrence of oil and gas along the coast of eastern Vietnam. SongHuong-BienDong(.) Petroleum System is identified in limited areas of the central and southern Song Hong basin. The major source rock of this petroleum system is Lower Miocene dark claystones of Song Huong formation which contain gas prone, type III kerogen. The migration model shows that hydrocarbons are generated from the Miocene source rocks in the center of the basin at about 12 Ma, and migrates updip through sand bodies of Quang Ngai formation to the major boundaries faults, and further moves into highly permeable up-dipping units, the Bien Dong formation. The best depiction of the generation-migration-accumulation of hydrocarbons occurs at about 2 Ma. The presence of the SongHuong-BienDong(.) Petroleum System is indicated by the large gas fields in the central and southern Song Hong Basin.

Son, Byeong-Kook; Thi Nguyen, Hong; Park, Mee-Sook

2014-05-01

275

Estrogen and Stroke Risk  

NSDL National Science Digital Library

"New study shows that a long period of estrogen deprivation can lead to loss of sensitivity and protective effects in the brain and can weaken areas normally resistant to stroke damage." This press release describes the experimental design and findings from the published study "Estrogen Attenuates Ischemic Oxidative Damage via an Estrogen Receptor alpha-Mediated Inhibition of NADPH Oxidase Activation" by Quang-Guang Zhang, Limor Raz, Ruimin Wang, Dong Han, Liesl De Sevilla, Fang Yang, Ratna K. Vadlamudi, and Darrell W. Brann in The Journal of Neuroscience, November 2009.

APS Communications Office (American Physiological Society Communications Office)

2009-11-03

276

Communications, customs, organization slowed project  

SciTech Connect

This paper reports that completion of a recently commissioned pipeline automation system in China overcame delays caused by serious difficulties in communications, organization, customs, and distance. The 153 mile, 28 in. hot-oil pipeline is located in the Shangdong Province, east of Beijing, China, and runs from Dongying, in the Shengli oil field, to the port of Huangdao on the Yellow Sea. The Shengli Oil Pipeline Co. (SOPC) now operates the fully automated Dong-Huang liquid pipeline with capability for central control in Weifang.

Blair, J.T. (Novacorp International Consulting Inc., Calgary (CA))

1992-09-21

277

An automated dual-gradient liquid chromatography-MS/MS method for the simultaneous determination of ferulic acid, ligustrazine and ligustilide in rat plasma and its application to a pharmacokinetic study.  

PubMed

An automated on-line SPE and innovative fast polarity switch bioanalysis method employing dual-gradient liquid chromatography (DGLC) coupled with mass spectrometry (DGLC-MS/MS) was established and validated for the simultaneous determination of ferulic acid, ligustrazine and ligustilide in rat plasma after administration of Rhizoma Chuanxiong, Angelica sinensis extract or monomer. The proteins in plasma samples were precipitated using acetonitrile: methanol (1:1, v/v). Sulfamethoxazole was used as an internal standard. The DGLC system contains two high-pressure pumps. The first pump was used for on-line solid phase extraction with a Cyclone™ SPE column. Chromatographic separations were performed with the other pump on a Syncronis C18 rapid analytical column. The analytical column was eluted by a gradient program that featured an acetonitrile/methanol/water gradient (flow-rate, 0.4ml/min). DGLC afforded greater convenience for bioanalysis. All analytes were simultaneously monitored in positive- and negative-ion mode by SRM (selective reaction monitoring) using the fast polarity switch speed of TSQ Vantage™. Method validation of the assay was implemented. No significant matrix effect was observed. The LLOQ of all analytes were <1.0ng/ml. The precision, recovery and linearity of the analysis met the pre-established requirements. The method was applied to the pharmacokinetics of ferulic acid, ligustrazine and ligustilide in Rhizoma Chuanxiong or Angelica sinensis extracts or monomers. PMID:24140450

Zeng, Mingfei; Zhang, Jing; Yang, Yifang; Jin, Yan; Xiao, Wei; Wang, Zhenzhong; Ding, Gang; Yan, Renjie

2014-01-25

278

Prescription pattern of chinese herbal products for breast cancer in taiwan: a population-based study.  

PubMed

Background. Chinese herbal products (CHPs) given as a therapy for symptom relief have gained widespread popularity among women with breast cancer. The aim of this study was to analyze the utilization of CHP among women with breast cancer in Taiwan. Methods. The usage, frequency of services, and CHP prescribed for breast cancer among women with breast cancer were evaluated, recruited from a randomly sampled cohort of 1,000,000 beneficiaries from the National Health Insurance Research Database. The logistic regression method was employed to estimate the odds ratios (ORs) for utilization of CHP. Results. 81.5 percent (N = 2, 236) of women with breast cancer utilized traditional Chinese medicine (TCM) and 18% of them sought TCM with the intent of treating their breast cancer. Jia-wei-xiao-yao-san (Augmented Rambling Powder) was the most frequently prescribed formula for treating breast cancer. Among the top 10 most frequently prescribed CHP for treating breast cancer, seven contained dang qui (Angelica sinensis-radix) and six contained ren shen (Panax ginseng-radix), which are reported to have potential beneficial synergistic effects on breast cancer cells. Conclusion. CHP containing dang qui (Angelica sinensis-radix) or ren shen (Panax ginseng-radix) are the most frequently prescribed for breast cancer and their effects should be taken into account by healthcare providers. PMID:22685488

Lai, Jung-Nien; Wu, Chien-Tung; Wang, Jung-Der

2012-01-01

279

Role of cuticular hydrocarbons of aphid parasitoids in their relationship to aphid-attending ants.  

PubMed

Lysiphlebus cardui, the dominant aphidiid parasitoid of the black bean aphid,Aphis fabae cirsiiacanthoidis (Afc), on creeping thistle, is able to forage in ant-attended aphid colonies without being attacked by ants. Several behavioral observations and experimental studies led to the hypothesis thatL. cardui mimics the cuticular hydrocarbon profile of its host aphid. Chemical analysis of the cuticular extracts revealed that bothL. cardui and Afc exclusively possess saturated hydrocarbons:n-alkanes, monomethyl (MMA), dimethyl (DMA), and trimethyl alkanes (TMA). Comparison of the hydrocarbon profiles of parasitoid and aphid showed great qualitative resemblance between parasitoid and host:L. cardui possesses almost all host-specific compounds in addition to species-specific hydrocarbons of mainly higher molecular weight (>C30). However, there is a lesser quantitative correspondence between parasitoid and host aphid. Furthermore, we analyzed the cuticular hydrocarbon profile of another parasitoid of Afc,Trioxys angelicae. This aphidiid species is vigorously attacked and finally killed by honeydewcollecting ants when encountered in aphid colonies. Its cuticular hydrocarbon profile is characterized by the presence of large amounts of (Z)-11-alkenes of chain lenghts C27, C29, C31, and C33, in addition to alkanes and presumably trienes. The role of the unsaturated hydrocarbons onT. angelicae as recognition cues for aphid-attending ants is discussed. PMID:24227578

Liepert, C; Dettner, K

1996-04-01

280

A parallel, randomized, double-blind, placebo-controlled study to investigate the effect of SagaPro on nocturia in men  

PubMed Central

Objective. This study aimed to investigate the effect of SagaPro, a product derived from Angelica archangelica leaf, on nocturia. Material and methods. Sixty-nine male patients 45 years or older with at least two nocturnal voids were randomized to receive SagaPro or placebo in a double-blind design for 8 weeks. Voiding diaries were assessed before and after the treatment. Results. The results indicate that SagaPro is safe. The actual number of nocturnal voids (ANV), nocturnal polyuria index (NPi) and nocturnal bladder capacity index (NBC index) decreased in the test population, but there was no significant difference between the treatment groups. Subsequent subgroup analysis showed that SagaPro significantly reduced the NBC index and nocturnal voids per sleeping hour in comparison to the placebo in participants with baseline NBC index above 1.3. When participants with sleep disorders were excluded from this group, ANV was also significantly reduced for the SagaPro group in comparison to the placebo group. Conclusion. SagaPro, made from an extract of the medicinal herb Angelica archangelica, is safe. This study did not show that SagaPro improved nocturia overall compared to placebo. Subgroup analysis suggested a beneficial effect in individuals with decreased nocturnal bladder capacity, which warrants further study.

Geirsson, Gudmundur; Gudmundsdottir, Hrefna; Egilsdottir, Perla B.; Gudbjarnason, Sigmundur

2013-01-01

281

Ultrasensitive Detection of Ferulic Acid Using Poly(diallyldimethylammonium chloride) Functionalized Graphene-Based Electrochemical Sensor  

PubMed Central

The electrochemical redox of ferulic acid (FA) was investigated systematically by cyclic voltammetry (CV) with a poly(diallyldimethylammonium chloride) functionalized graphene-modified glassy carbon electrode (PDDA-G/GCE) as a working electrode. A simple and sensitive differential pulse voltammetry (DPV) technique was proposed for the direct quantitative determination of FA in Angelica sinensis and spiked human urine samples for the first time. The dependence of the intensities of currents and potentials on nature of the supporting electrolyte, pH, scan rate, and concentration was investigated. Under optimal conditions, the proposed sensor exhibited excellent electrochemical sensitivity to FA, and the oxidation peak current was proportional to FA concentration in the range of 8.95 × 10?8?M ~5.29 × 10?5?M, with a relatively low detection limit of 4.42 × 10?8?M. This fabricated sensor also displayed acceptable reproducibility, long-term stability, and high selectivity with negligible interferences from common interfering species. Besides, it was applied to detect FA in Angelica sinensis and biological samples with satisfactory results, making it a potential alternative tool for the quantitative detection of FA in pharmaceutical analysis.

Liu, Lin-jie; Gao, Xia; Zhang, Pei; Feng, Shi-lan; Hu, Fang-di; Li, Ying-dong; Wang, Chun-ming

2014-01-01

282

Inhibitory potential of herbal medicines on human cytochrome P450-mediated oxidation: properties of umbelliferous or citrus crude drugs and their relative prescriptions.  

PubMed

To investigate the possible drug interaction with herbal medicine, hot water decoctions or 40% ethanol infusions of several Umbelliferous or Citrus crude drugs and their prescriptions were examined in vitro for their abilities to inhibit human cytochrome P450 3A (CYP3A). Addition of each decoction or infusion from Baizhi (Angelica dahurica and varieties), Qianghuo (Notopterygium incisum or N. forbesii), Duhuo (Angelica biserrata), Fangfeng (Saposhnikovia divaricata), Danggui (Angelicasinensis), Zhishi or Zhiqiao (Citrus aurantium) resulted in various degrees of human CYP3A inhibition as determined by microsomal testosterone 6beta-hydroxylation. The inhibitory potency was consistent with the abundance of the hydrophobic components for each sample. Experiments on the infusion of a Japanese Baizhi (BZ1) showed the major role of furanocoumarins on human CYP3A inhibition. Some of the crude drugs and a related prescription showed increased inhibition after the preincubation, suggesting the involvement of a mechanism-based inhibition. Some formulated prescriptions, however, showed intense inhibition with their hydrophobic fractions rather than with their hydrophobic fractions, suggesting that components other than furanocoumarins in herbal prescriptions may also cause CYP3A inhibition. These results indicate the necessity of intensive investigations on the possible drug interaction with traditional medicines. PMID:11388644

Guo, L Q; Taniguchi, M; Chen, Q Y; Baba, K; Yamazoe, Y

2001-04-01

283

Influence of traditional Chinese anti-inflammatory medicinal plants on leukocyte and platelet functions.  

PubMed

The enzymes 5-lipoxygenase and elastase are therapeutic targets in dermatological disorders such as psoriasis. Fifteen extracts from traditional Chinese medicinal plants used to treat topical inflammations were screened for their inhibitory effect on lipoxygenase, cyclooxygenase and elastase activity in intact leukocytes and platelets. Astragalus membranaceus, Forsythia suspensa and Poria cocos inhibited 5-lipoxygenase, with IC50 values of 141, 80 and 141 microg mL(-1), respectively. The latter two species, along with Angelica dahurica and Angelica pubescens, also inhibited elastase (IC50 values of 80, 123, 68 and 93 microg mL(-1), respectively), while A. pubescens, Atractylodes macrocephala, Lentinus edodes, Rehmannia glutinosa and Paeonia lactiflora selectively inhibited 12-(S)-HHTrE production, a valid marker of cyclooxygenase activity. The inhibition of phospholipase A(2) activity by P. cocos is discussed. Dehydrotumulosic and pachymic acids, which have been isolated from P. cocos, were shown to inhibit leukotriene B(4) release. The results indicate that both P. cocos and F. suspensa are potentially valuable species in the management of skin pathologies involving chronic inflammation. PMID:14604471

Prieto, J M; Recio, M C; Giner, R M; Máñez, S; Giner-Larza, E M; Ríos, J L

2003-09-01

284

On-line concentration by field-enhanced sample injection with reverse migrating micelles in micellar electrokinetic capillary chromatography for the analysis of coumarins from traditional Chinese medicine and human serum.  

PubMed

In this work, a simple, reproducible and sensitive micellar electrokinetic chromatography method was developed for the separation and determination of three coumarins, imperatorin (IM), isoimperatorin (IO) and osthole (OS) from traditional Chinese medicine and human serum. Field-enhanced sample injection with reverse migrating micelles was used for on-line concentration of the coumarins. The optimum buffer contained 50 mM H(3)PO(4), 160 mM sodium dodecyl sulfate, 20% acetonitrile and 15% 2-propanol, and the pH of buffer was 2.0. The sample solution was diluted with water containing 5 mM sodium dodecyl sulfate and injected for 15 s with -8 kV after injection of 2 s water plug. The effects of concentrations of sodium dodecyl sulfate and organic modifier, the sample matrix, the injection time of water plug, the injection voltage and injection time of sample on the separation and stacking efficiency were investigated. Under the optimum conditions, the analytes were well separated and by optimizing the stacking conditions, about 93, 195 and 136 fold improvement in the detection sensitivity was obtained for IM, IO and OS. The contents of three coumarins in Angelica dahurica Benth, Radix Angelicae Pubescentis and Fructus Cnidii were successfully determined with satisfactory repeatability and recovery. The possibilities of using this method for the determination of three coumarins in spiked human serum were also tested. PMID:19795362

Jiang, Ting-Fu; Lv, Zhi-Hua; Wang, Yuan-Hong; Yue, Mei-E

2010-06-01

285

Comparison of a novel distillation method versus a traditional distillation method in a model gin system using liquid/liquid extraction.  

PubMed

This research studied a novel form of distillation (high vacuum distillation) as a method for preserving volatile aroma chemicals important to the organoleptic attributes of a four botanical model gin as well as the degradation products generated during the heating required in traditional methods of gin distillation. A 2 (5) factorial experiment was conducted in a partially confounded incomplete block design and analyzed using the PROC MIXED procedure from SAS. A model gin was made of dried juniper berries (Juniperus communis), coriander seed (Coriandrum sativum), angelica root (Angelica archangelica), and dry lemon peel (Citrus limonum). This was distilled on a traditional still utilizing atmospheric pressure and a heating mantel to initiate phase separation as well as a novel still (high vacuum) utilizing high vacuum pressures below 0.1 mmHg and temperatures below -15 degrees C to initiate phase separation. The degradation products (alpha-pinene, alpha-phellandrene, E-caryophyllene, and beta-myrcene) were present at greater levels (approximately 10 times) in the traditional still-made gin as compared to the novel gin. PMID:18771269

Greer, Derek; Pfahl, Les; Rieck, Jim; Daniels, Tim; Garza, Oscar

2008-10-01

286

Auraptenol attenuates vincristine-induced mechanical hyperalgesia through serotonin 5-HT1A receptors  

PubMed Central

Common chemotherapeutic agents such as vincristine often cause neuropathic pain during cancer treatment in patients. Such neuropathic pain is refractory to common analgesics and represents a challenging clinical issue. Angelicae dahuricae radix is an old traditional Chinese medicine with demonstrated analgesic efficacy in humans. However, the active component(s) that attribute to the analgesic action have not been identified. This work described the anti-hyperalgesic effect of one coumarin component, auraptenol, in a mouse model of chemotherapeutic agent vincristine-induced neuropathic pain. We reported that auraptenol dose-dependently reverted the mechanical hyperalgesia in mice within the dose range of 0.05–0.8?mg/kg. In addition, the anti-hyperalgesic effect of auraptenol was significantly blocked by a selective serotonin 5-HT1A receptor antagonist WAY100635 (1?mg/kg). Within the dose range studied, auraptenol did not significantly alter the general locomotor activity in mice. Taken together, this study for the first time identified an active component from the herbal medicine angelicae dahuricae radix that possesses robust analgesic efficacy in mice. These data support further studies to assess the potential of auraptenol as a novel analgesic for the management of neuropathic pain.

Wang, Yunfei; Cao, Shu-e; Tian, Jianmin; Liu, Guozhe; Zhang, Xiaoran; Li, Pingfa

2013-01-01

287

Antidepressant-like effects of auraptenol in mice  

PubMed Central

Depression is a major psychiatric disorder affecting nearly 21% of the world population and imposes a substantial health burden on society. Current available antidepressants are not adequate to meet the clinical needs. Here we report that auraptenol, an active component of the traditional Chinese medicine, angelicae dahuricae radix, had antidepressant-like effects in mice models of depression. In mouse forced swimming test and tail suspension test, two validated models of depression, auraptenol dose-dependently decreased the immobility duration within the dose range of 0.05–0.4?mg/kg. In addition, the antidepressant-like effects of auraptenol was significantly averted by a selective serotonin 5-HT1A receptor antagonist WAY100635 (1?mg/kg). These doses that affected the immobile response did not affect locomotor activity. In summary, this study for the first time identified an active component from the herbal medicine angelicae dahuricae radix that possesses robust antidepressant-like efficacy in mice. These data support further exploration for the possibility of developing auraptenol as a novel antidepressant agent in the treatment of major depression disorders.

Gu, Xiaosu; Zhou, Yong; Wu, Xiaomei; Wang, Fen; Zhang, Cai-Yi; Du, Chenchen; Shen, Lihua; Chen, Xiang; Shi, Jiansheng; Liu, Chunfeng; Ke, Kaifu

2014-01-01

288

Inhibition of sulfide mineral oxidation by surface coating agents: batch and field studies.  

PubMed

The potential of several surface coating agents to inhibit the oxidation of metal sulfide minerals from Young-Dong coal mine and the Il-Gwang gold mine was examined by conducting laboratory scale batch experiments and field tests. Powdered pyrite as a standard sulfide mineral and rock samples from two mine outcrops were mixed with six coating agents (KH(2)PO(4), MgO and KMnO(4) as chemical agents, and apatite, cement and manganite as mineral agents) and incubated with oxidizing agents (H(2)O(2) or NaClO). For the observed time period (8 days), Young-Dong coal mine samples exhibited the least sulfate (SO(4)(2-)) production in the presence of KMnO(4) (16%) or cement (4%) while, for Il-Gwang mine samples, the least SO(4)(2-) production was observed in presence of KH(2)PO(4) (8%) or cement (2%) compared to control. Field-scale pilot tests at the Il-Gwang site also showed that addition of KH(2)PO(4) decreased SO(4)(2-) production from 200 to 13 mg L(-1) and it also reduced Cu and Mn from 8 and 3 mg L(-1), respectively to <0.05 mg L(-1) (below ICP-OES detection limits). The experimental results suggested that the use of surface coating agents is a promising alternative for sulfide oxidation inhibition at acid mine drainage sites. PMID:22727481

Ji, Min-Kyu; Gee, Eun-Do; Yun, Hyun-Shik; Lee, Woo-Ram; Park, Young-Tae; Khan, Moonis Ali; Jeon, Byong-Hun; Choi, Jaeyoung

2012-08-30

289

Magnetic and orbital order in (RMnO3)n/(AMnO3)2n superlattices  

NASA Astrophysics Data System (ADS)

The magnetic and orbital orders in (RMnO3)n/(AMnO3)2n (R: rare earths; A: alkaline earths, n=1 and 2) superlattices have been studied using both the double-exchange model and density functional theory calculations. For large bandwidth manganites, the A-type antiferromagnetic order is found to be robust when the superlattices are grown on a SrTiO3 substrate, as in recent experiments on (LaMnO3)n/(SrMnO3)2n. In addition, a C-type antiferromagnetic state is predicted for these superlattices when using substrates like LaAlO3 with smaller lattice constants. The physical mechanism for the stabilization of the A- and C- magnetic transitions is driven by the orbital splitting of the x2-y2 and 3z2-r2 orbitals, which is induced by the Q3 mode of Jahn-Teller distortions created by the strain induced by the substrates. If the superlattices were prepared employing narrow bandwidth manganites, several non-homogeneous magnetic profiles are predicted to exist, highlighting the importance of carrying out investigations in this mostly unexplored area of research. [1] S. Dong, Q.F. Zhang, S. Yunoki, J.-M. Liu, and E. Dagotto, Phys. Rev. B in press. (ArXiv: 1211.1943) [2] Q.F. Zhang, S. Dong, B.L. Wang, and S. Yunoki, Phys. Rev. B 86, 094403 (2012).

Dong, Shuai; Zhang, Qinfang; Dagotto, Elbio

2013-03-01

290

Retrieval of continental and Marine Low-level cloud microphysical properties: Challenge and Difficulty to retrieve its profile  

NASA Astrophysics Data System (ADS)

Xiquan Dong (dong@aero.und.edu) and Baike Xi, University of North Dakota Knowledge of the vertical structure of a cloud's microphysical characteristics is important for a variety of reasons. The profiles of cloud-droplet effective radius (re) and liquid water content (lwc) affect the cloud's interaction with solar and infrared radiation that ultimately contribute to the energy budget at the surface and top of atmosphere (TOA). Knowledge of the microphysical structure also increases our understanding of the process acting to form and maintain these cloud systems. This understanding may lead to improved numerical models and parameterizations of clouds. Several algorithms have been developed in recent years that combine millimeter wavelength radar (MMCR) observations with other data sources to derive the microphysical properties of low-level stratus clouds. The multi-parameter algorithms developed for low-level liquid-phase stratus clouds use radar reflectivity observations and microwave radiometer-derived cloud liquid water path (lwp) combined with various assumptions to retrieve the microphysical properties of the cloud layer. Since these techniques tend to assume a functional form (lognormal or gamma) for the particle size distribution, three independent parameters are generally needed to estimate the characteristics of the particle size distribution. In this study, we will investigate the strengths and weaknesses of different retrieval methods using the aircraft in-situ measurements as a ground-truth.

Dong, X.

2013-12-01

291

US Naval Forces, Vietnam Monthly Historical Supplement for November 1966.  

National Technical Information Service (NTIS)

Contents: Chronology of Significant Events; Operation QUAI VAT; River Patrol Force: Arrival of GAME WARDEN Support Ship (LST-846), Delta River Patrol Group, Rung Sat Special Zone Patrol Group, GAME WARDEN Unit Changes. Coastal Surveillance Force: Northern...

1967-01-01

292

Use of Yokukansan (TJ-54) in the treatment of neurological disorders: a review.  

PubMed

Kampo herbal remedies are reported to have a wide range of indications and have attracted attention due to reports suggesting that these remedies are effective when used in disease treatment while maintaining a favourable quality of life. Yokukansan, also known as TJ-54, is composed of seven herbs; Angelica acutiloba, Atractylodes lancea, Bupleurum falcatum, Poria cocos, Glycyrrhiza uralensis, Cnidium officinale and Uncaria rhynchophylla. Yokukansan is used to treat insomnia and irritability as well as screaming attacks, sleep tremors and hypnic myoclonia, and neurological disorders which include dementia and Alzheimer's disease - the focus of this article. It is concluded that Yokukansan is a versatile herbal remedy with a variety of effects on various neurological states, without reported adverse effects. Traditional herbal medicines consist of a combination of constituents which account for the clinical effect seen. Likewise, the benefits of Yokukansan are probably attributable to the preparation as a whole, rather than to individual compounds. PMID:20812276

de Caires, S; Steenkamp, V

2010-09-01

293

Bisabolangelone inhibits dendritic cell functions by blocking MAPK and NF-?B signaling.  

PubMed

Bisabolangelone (BISA), isolated from the roots of Angelica koreana, has many pharmacological activities, such as anti-tumor, anti-microbial, antioxidant, and anti-inflammatory activities. In this study, we investigated the anti-inflammatory mechanisms of BISA in dendritic cells (DCs), which play an essential role in innate and adaptive immune responses. BISA attenuated the production of pro-inflammatory cytokines including interleukin (IL)-12, IL-1?, and tumor necrosis factor-alpha (TNF-?), migration to macrophage inflammatory protein-3 beta, and allo-T cell activating ability of DCs. In addition, BISA affected endocytosis of DCs. Molecular studies showed that BISA suppressed MAPK phosphorylation and nuclear translocation of NF-?B p50/p65. Taken together, our data suggest that BISA inhibited DC functions by blocking MAPK and NF-?B signaling. PMID:23727177

Kim, Hyung Sook; Lee, Young June; Lee, Hong Kyung; Kim, Ji Sung; Park, Yunsoo; Kang, Jong Soon; Hwang, Bang Yeon; Hong, Jin Tae; Kim, Youngsoo; Han, Sang-Bae

2013-09-01

294

Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping--enzymatic digestion followed by chromatographic analysis.  

PubMed

Polysaccharides isolated from traditional Chinese medicines (TCMs) exhibit multiple pharmacological activities. However, quality control of polysaccharides is a challenge because of their complicate structure and macro-molecular mass. In this study, saccharide mapping based on specific enzymatic digestion of polysaccharides and chromatographic analysis was proposed to discriminate the polysaccharides from different TCMs. Endo-carbohydrase such as glucanase, arabinanase, xylanase, galactanase, cellulase, amylase and pectinase were used for enzymatic digestion of polysaccharides from 9 TCMs namely Panax ginseng, P. notoginseng, P. quinquefolium, Cordyceps sinensis, C. militaris, Ganoderma lucidum, G. sinense, Astragalus membranaceus and Angelica sinensis. By using high performance size exclusion chromatography (HPSEC) as well as derivatization with 1-Phenyl-3-methyl-5-pyrazolone (PMP) and HPLC analysis, the enzymatic hydrolysis properties of polysaccharides and their saccharide mapping were determined. The polysaccharides from 9 TCMs were firstly successfully distinguished based on their characteristic saccharide maps, which is helpful to improve the quality control of polysaccharides. PMID:19850432

Guan, J; Li, S P

2010-02-01

295

Herbal Extract SH003 Suppresses Tumor Growth and Metastasis of MDA-MB-231 Breast Cancer Cells by Inhibiting STAT3-IL-6 Signaling  

PubMed Central

Cancer inflammation promotes cancer progression, resulting in a high risk of cancer. Here, we demonstrate that our new herbal extract, SH003, suppresses both tumor growth and metastasis of MDA-MB-231 breast cancer cells via inhibiting STAT3-IL-6 signaling path. Our new herbal formula, SH003, mixed extract from Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii Maximowicz, suppressed MDA-MB-231 tumor growth and lung metastasis in vivo and reduced the viability and metastatic abilities of MDA-MB-231 cells in vitro. Furthermore, SH003 inhibited STAT3 activation, which resulted in a reduction of IL-6 production. Therefore, we conclude that SH003 suppresses highly metastatic breast cancer growth and metastasis by inhibiting STAT3-IL-6 signaling path.

Woo, Sang-Mi; Park, Sunju; Shin, Yong Cheol; Ko, Seong-Gyu

2014-01-01

296

Antiinflammatory activity of an herbal preparation (HemoHIM) in rats.  

PubMed

This study evaluated a new herbal preparation, HemoHIM, for its antiinflammatory activity against carrageenan-induced edema, the formation of granulation tissues by cotton pellet and experimental colitis by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The HemoHIM was prepared by adding its ethanol-insoluble polysaccharide fraction to the total water extract of Angelica Radix, Cnidii Rhizoma and Paeonia Radix. The preparation (4 mg of solids/mL of drinking water, p.o., 50-100 mg/kg of body weight, i.p.) produced a dose-related inhibition of carrageenan-induced paw edema and cotton pellet-induced granuloma in rats. In addition, HemoHIM also reduced the degree of TNBS-induced colitis and improved the gross and histological changes such as thickening, dilatation, ulceration, and infiltration by polymorphonuclear leukocytes and multiple erosive lesions. These results demonstrate that the HemoHIM has a potent antiinflammatory effect. PMID:17486680

Jo, Sung Kee; Lee, Hae June; Kim, Se Ra; Kim, Jong Choon; Bae, Chun Sik; Jung, Uhee; Park, Hae Ran; Jang, Jong Sik; Kim, Sung Ho

2007-07-01

297

[Determination of geographical origins of Chinese medical herbs by NIR and pattern recognition].  

PubMed

Geographical origin of medical herbs is an important factor of the quality of many traditional Chinese herbal medicines. The objective of the present study is to investigate whether NIR spectroscopy coupled with pattern recognition techniques could effectively discriminate geographical origins of medical herbs. Nearest neighbor method (NNM) and a SVM-based multiclass classifier were employed to discriminate 269 Angelicae Dahuricae Radix (ADR) samples from 4 provinces and 380 Salviae Miltiorrhizae Radix (SMR) samples from 6 provinces in China. The multiclass classifier achieves leave-one-out cross-validation accuracy of 99% for (ADR) and 95% (SMR). This classification scheme can be a highly accurate approach to the rapid and nondestructive discrimination of medical herbs of different origins. PMID:16836125

Liu, Shu-hua; Zhang, Xue-gong; Zhou, Qun; Sun, Su-qin

2006-04-01

298

[Use of FTIR and pattern recognition to determine geographical origins of Chinese medical herbs].  

PubMed

Geographical origin of medical herbs is an important factor of the quality of many traditional Chinese herbal medicines. The objective of this study is to investigate whether FTIR spectroscopy coupled with pattern recognition techniques could effectively discriminate geographical origins of medical herbs. Nearest neighbor method (NNM) and a SVM-based multiclass classifier were employed to discriminate 269 angelicae dahuricae radix (ADR) samples from 4 provinces in China and 380 salviae miltiorrhizae radix (SMR) samples from 6 provinces. A leave-one-out cross-validation accuracy of 99% was achieved by the multiclass classifier. The study shows this classification scheme can be a highly accurate approach for the discrimination of medical herbs of different origins. PMID:16201362

Liu, Shu-hua; Zhang, Xue-gong; Zhou, Qun; Sun, Su-qin

2005-06-01

299

Anti-acne effects of Oriental herb extracts: a novel screening method to select anti-acne agents.  

PubMed

The acne-therapeutic effects of Oriental herb extracts were investigated in terms of antichemotactic effect on polymorphonuclear leucocytes, antilipogenic actions, antibacterial activity against Propionibacterium acnes and resistance induction potency in the bacteria. The ethanol extract (0.01%) of Angelica dahurica markedly suppressed neutrophil chemotaxis, comparable to the effect of erythromycin (0.01%), whereas a strong antilipogenic effect was obtained with rhizoma coptidis (Coptis chinensis) extract (0.01%), leading to a higher efficacy than that of retinoic acid (0.01%). Interestingly, only Glycyrrhiza glabra showed a remarkable antibacterial activity against P. acnes, resulting in negligible induction of resistance, in comparison with a marked development of resistance in the bacteria treated with erythromycin. We suggest that an appropriate formulation containing A. dahurica, rhizoma coptidis and G. glabra could be helpful for the prevention and treatment of acne lesions. PMID:12637783

Nam, C; Kim, S; Sim, Y; Chang, I

2003-01-01

300

Lead compounds for anti-inflammatory drugs isolated from the plants of the traditional oriental medicine in Korea.  

PubMed

Effects of compounds isolated from medicinal plants in Korea on prostaglandin E2 (PGE2) production in rat peritoneal macrophages were examined, and mechanism of action of the active constituents was analyzed. The active constituents were as follows; tectorigenin and tectoridin isolated from the rhizomes of Belamcanda chinensis, platycodin D isolated from the roots of Platycodon grandiflorum, imperatorin isolated from the roots of Angelica dahurica, and hyperin isolated from the roots of Acanthopanax chiisanensis. These compounds inhibit the induction of cyclooxygenase-2 (COX-2), thus inhibiting PGE2 production. The chemically synthesized chalcone derivative, 2'-hydroxy-4'-methoxychalcone, also inhibits PGE2 production by suppressing COX-2 induction. In contrast, taiwanin C isolated from the roots of Acanthopanax chiisanensis inhibited PGE2 production by direct inhibition of COX-1 and COX-2. PMID:18782027

Hong, JangJa; Shin, Kuk Hyun; Lim, Soon Sung; Kwak, Jong Hwan; Zee, OkPyo; Ishihara, Kenji; Hirasawa, Noriyasu; Seyama, Toshio; Ohuchi, Kazuo

2008-09-01

301

Effect of oral imperatorin on memory in mice.  

PubMed

The aim of this study was to explore the effect of the acetylcholinesterase inhibiting mixture of extracts of Angelica archangelica fruit and Geranium sylvaticum on memory. Furthermore the effect of the main compound, the furanocoumarin imperatorin, which has been shown to affect several neurotransmitters, was studied. Passive avoidance was measured by step-down latency and step-through latency of 10 months old mice receiving 0.79 mg/kg of imperatorin daily, pure or as part of the extracts, for 14 days or longer. Step-down latency was significantly higher in both groups receiving imperatorin than in the control group. In contrast, no difference was found between treatment groups regarding step-through latency. The results indicate that the imperatorin is the main active component of the extract mixture. PMID:24140410

Sigurdsson, Steinthor; Gudbjarnason, Sigmundur

2013-11-15

302

Suppression of voltage-gated Na(+) channels and neuronal excitability by imperatorin.  

PubMed

Imperatorin is a naturally occurring furocoumarin compound isolated from plants such as Angelica archangelica and Cnidium monnieri. It has multiple pharmacological effects including anticonvulsant effects. Here we determined the effects of imperatorin on voltage-gated Na(+) channels (VGSC) using whole-cell patch clamp techniques in differentiated neuronal NG108-15 cells. We showed that imperatorin inhibited VGSC; such inhibition did not show state-dependence. Imperatorin caused a left shift in the steady-state inactivation curve without affecting activation gating. The inhibition of VGSC by imperatorin displayed a mild frequency-dependence. Imperatorin was also shown to inhibit VGSC and action potential amplitude without affecting voltage-gated K(+) channels in rat hippocampal CA1 neurons. In conclusion, our results suggest that imperatorin dampens neuronal excitability by inhibiting VGSC. PMID:24113522

Wu, King-Chuen; Chen, Yi-Hung; Cheng, Ka-Shun; Kuo, Yueh-Hsiung; Yang, Chin-Tsang; Wong, Kar-Lok; Tu, Yuan-Kun; Chan, Paul; Leung, Yuk-Man

2013-12-01

303

The Effect of Z-Ligustilide on the Mobility of Human Glioblastoma T98G Cells  

PubMed Central

Z-ligustilide (LIG), an essential oil extract from Radix Angelica sinensis, has broad pharmaceutical applications in treating cardio-vascular diseases and ischemic brain injury. Recently, LIG has been connected to Glioblastoma multiforme (GBM) because of its structural similarity to 3-n-alkyphthalide (NBP), which is specifically cytotoxic to GBM cells. Hence, we investigated LIG’s effect on GBM T98G cells. The study shows that LIG can significantly reduce T98G cells’ migration in a dose-dependent manner. Furthermore, the attenuation of cellular mobility can be linked to the activity of the Rho GTPases (RhoA, Rac1 and Cdc42), the three critical molecular switches governing cytoskeleton remodeling; thus, regulating cell migration. LIG significantly reduces the expression of RhoA and affects in a milder manner the expression of Cdc42 and Rac1.

Yin, Jun; Wang, Chengyuan; Mody, Aaron; Bao, Lin; Hung, Shen-Hsiu; Svoronos, Spyros A.; Tseng, Yiider

2013-01-01

304

[Comparison on content of ligustilides in different danggui samples].  

PubMed

Bioactivity of Danggui is linked to the content of ligustilide, but the relationship between ligustilide with herb shape, cultivating areas and plant species is still unknown. The relationship was investigated by quantifying on the amounts of Z-ligustilide and E-ligustilide by HPLC-DAD-MS method, and then comparing the content of ligustilides (the sum of Z-ligustilide and E-ligustilide) among forty-four various "Danggui" samples containing thirty Chinese Danggui (CDG), six Japanese Danggui (JDG), four Korea Danggui (KDG) and four European Danggui (EDG). Results showed that the content of ligustilides in CDG samples (Angelica sinensis) was in the range of 5.63-24.53 mg x g(-1) with the mean of 11.02 mg x g(-1) (n = 30). Ligustilides amounts were varied among samples cultivated in different areas in China, i. e. 13.90 mg x g(-1) (n = 6) in Yannan, 12.51 mg x g(-1) (n = 6) in Sichuan and 10.04 mg x g(-1) (n = 13) in Gansu. It was also found that ligustilides content was related to the shape, color and fragrance of herb, e. g. the relative larger amount of ligustilides was in the small main root, long rootlet and perfumed sample. Further, ligustilides contents were estimated to be 1.00 mg x g(-1) (n = 6) in JDG samples (A. acutiloba and A. acutiloba var. sugiyamae) and 2.78 mg x g(-1) (n = 2) in EDG samples (lovage root, Levisticum officinale). However, ligustilides could not be detected in the four KDG samples (A. gigas) and two EDG samples (angelica root, A. archangelica). It has been concluded that ligustilide is significant variant among plant species, which may result in the variety of bioactivity and therapeutic effect. PMID:24380307

Li, Xi; Zhang, Li-Hong; Lv, Guang-Hua; Wang, Xiao-Xiao; Jiang, Wei-Dong; Jin, Yu-Qing; Zhao, Zhong-Zhen

2013-09-01

305

Synergistic promotion of blood vessel regeneration by astragaloside IV and ferulic acid from electrospun fibrous mats.  

PubMed

The promotion of blood vessel initiation and growth plays an important role in the realization of therapeutic vascularization and regeneration of functional tissues. Astragalus membranaceus and angelica sinensis are commonly used traditional Chinese medicines for enriching the blood. In the current study astragaloside IV (AT, the main active ingredient of astragalus) and ferulic acid (FA, the main ingredient of angelica) were loaded into electrospun fibrous scaffolds to provide abundant and sustained biological factors required to initiate vascularization and bring it to maturity. The cell viability after AT and FA treatment was dose-dependent with an optimal concentration of around 50 ?g/mL, and the most significant synergistic effect was demonstrated for the combined treatment with AT and FA with the ratio of 7/3 on both primary endothelial and smooth muscle cells. The in vitro release study showed that the amount of AT and FA release could be regulated by their loading amount and ratios in electrospun fibers. The localized and sustained codelivery of AT and FA indicated significantly high cell viability and secretion of extracellular matrices for both endothelial and smooth muscle cells, and induced significantly high densities of vascular structures after subcutaneous implantation. The most significant angiogenesis promotion with few inflammatory reactions was demonstrated for electrospun fibers containing AT and FA with the ratio of 7/3. It was suggested that the integration of the synergistic effect of Chinese medicine into electrospun fibrous scaffolds should provide clinical relevance for therapeutic vascularization, full vascularization in engineered tissues, and regeneration of blood vessel substitutes. PMID:23651405

Wang, Huan; Zhang, Yun; Xia, Tian; Wei, Wei; Chen, Fang; Guo, Xueqin; Li, Xiaohong

2013-06-01

306

Biopharmaceutical characterization of decursin and their derivatives for drug discovery.  

PubMed

Angelica gigas Nakai and its components are known to have neuroprotective, antiplatelet, and anticancer activities. The present study evaluated the in vitro and in vivo biopharmaceutical characterization of Angelica gigas component substances, including decursin (the main substance), decursinol angelate (decursin isomer), JH714 (ether form of decursin) and epoxide decursin (epoxide form of decursin). Decursin, decursinol angelate and JH714 exhibited acceptable metabolic stability (>50%) in liver microsomes from human and higher bound fraction (>90%) in human plasma operating ultrafiltration. Decursin and decursinol angelate in CYP1A2 and CYP2C19 indicated less than 50% CYP activity, suggesting inhibition of the CYP isoforms using Vivid® CYP screening kit. JH714 only showed an apparent permeability coefficient of <10?×?10?? cm/s in MDCK cells, suggesting that it is poorly absorbed. Blood brain barrier permeability was examined after oral administration to male Sprague-Dawley (SD) rats, and pharmacokinetic studies were performed after oral and intravenous administration of 10?mg/kg compounds. Decursin, decursinol angelate and JH714 showed ratios of compound concentration in brain with respect to plasma (Cbrain/Cplasma) of >1.5, suggesting good brain/plasma ratio at 0.5, 1, 3, and 5?h. In contrast, Cbrain/Cplasma was <0.5 for epoxide decursin. For all test compounds, >1.5% of the dose remained in GI tract after 8?h, and the excretion rate in urine was <0.5% which suggests that gastro intestinal tract may be major site of disposition following oral administration. Finally, these results may be useful for the design of dosage regimens of decursin and its derivatives. PMID:23003006

Mahat, Bimit; Chae, Jung-woo; Baek, In-hwan; Song, Gyu-yong; Song, Jin-sook; Ma, Jin-yeul; Kwon, Kwang-il

2013-10-01

307

The effects of imperatorin on anxiety and memory-related behavior in male Swiss mice.  

PubMed

The purpose of the reported experiments was to examine the effects of imperatorin [9-(3-methylbut-2-enyloxy)-7H-furo[3,2-g]chromen-7-one], a bioactive furanocoumarin isolated from the fruits of Angelica archangelica (Angelica officinalis) on anxiety and memory-related behaviors of mice. Male Swiss mice were tested for anxiety and cognition, in the elevated plus maze test (EPM), using two different procedures. In the present experiments, imperatorin was administered acutely (at the doses of 5, 10, 20, 30, and 50 mg/kg); injections were made 15, 30, and 60 min before test (anxiety); 30 min before the first trial (memory acquisition); or immediately after the first trial (memory consolidation), as well as subchronically, twice a day for 6 days. On the seventh day, the mice were injected once with imperatorin (10 and 20 mg/kg, i.p.) 30 min before the test (anxiety) and 30 min before the first trial (memory acquisition), or immediately after the first trial (memory consolidation). We observed that imperatorin when administered acutely and repeatedly, at the doses of 10 and 20 mg/kg, exerted an anxiolytic effect on mice tested 30 min after the injection measured in the EPM test. By contrast, no such effect was observed after the acute administration of imperatorin at the doses of 5, 30 and 50 mg/kg. Moreover, other observations carried out 15 and 60 min after a single injection of the drug did not reveal any effect of imperatorin on anxiety behavior in the EPM test. Furthermore, acute and repeated administration of imperatorin (10 and 20 mg/kg) improved different stages of memory processes (both acquisition and consolidation) in a modified EPM test (mEPM). The results of our research suggest imperatorin to be an interesting therapeutical option in disorders with high anxiety levels and memory impairment. PMID:22686497

Budzynska, Barbara; Kruk-Slomka, Marta; Skalicka-Wozniak, Krystyna; Biala, Grazyna; Glowniak, Kazimierz

2012-08-01

308

Monitoring Geocenter and Scale Variations Using DORIS Data: Monthly and Weekly Comparisons Toward ITRF References  

NASA Astrophysics Data System (ADS)

Since 1992, the Institut Geographique National is using the GIPSY/OASIS II Software, developped by the Jet Propulsion Laboratory, for DORIS data processing [Willis et al, 1994]. Main applications are precise geodetic positioning for the stations of the ground tracking network, precise orbit determination (Topex/Posei'don) and also polar motion monitoring in the scope of the International Earth Rotation Service (IERS) [Willis 1996a; Willis 1997]. Within the Geocenter campaign organized by the IERS, IGN has derived monthly and weekly coordinates data sets from DORIS data analysis than can be used to estimate possible variationsin location of the geocenter. Several authors [Stolz 1976; Dong 1997] have shown, than due to geophysical changes in the geographical distribution of the Earth masses, the Earth center of mass should have some small variations mostly at an annual period.

Willis, Pascal; Sillard, Patrick

309

Uniqueness of boundary blow-up solutions on exterior domain of RN  

NASA Astrophysics Data System (ADS)

In this paper, we consider the existence and uniqueness of positive solutions of the degenerate logistic type elliptic equation where N[greater-or-equal, slanted]2, D[subset of]RN is a bounded domain with smooth boundary and a(x), b(x) are continuous functions on RN with b(x)[greater-or-equal, slanted]0, b(x)[not identical with]0. We show that under rather general conditions on a(x) and b(x) for large x, there exists a unique positive solution. Our results improve the corresponding ones in [W. Dong, Y. Du, Unbounded principal eigenfunctions and the logistic equation on RN, Bull. Austral. Math. Soc. 67 (2003) 413-427] and [Y. Du, L. Ma, Logistic type equations on RN by a squeezing method involving boundary blow-up solutions, J. London Math. Soc. (2) 64 (2001) 107-124].

Dong, Wei; Pang, Changci

2007-06-01

310

The French Atlantic littoral and the Massif Armoricain, part 1. [Island of Jersey, Fromentine, Loire Estuary, and Mont Saint-Michel Bay  

NASA Technical Reports Server (NTRS)

The author has identified the following significant results. For interpretation of Isle of Jersey imagery, two types of taxons were defined according to their variability in time. On the whole, taxons with a similar spectral signature were opposed to those with strongly varying spectral signature. The taxon types were low diachronic variations and strong diachronic variation. Imagery interpretation was restricted to the landward part of the Fromentine area, including the sand beaches which were often difficult to spectrally separate from the barren coastal dunes in the southern part of Noirmoutier Island as well as along the Breton marsh. From 1972 to 1976, sandbanks reduced in area. Two high river discharge images showed over a two year period an identical outline for the Bilho bank to seaward, whereas upstream, the bank has receeded in the same time to a line joining Paimboeuf to Montoir. The Brillantes bank has receeded at both ends, partly due to dredging operations in the access channel to Donges harbor.

Verger, F. (principal investigator); Monget, J. M.; Guerin, O.; Poisson, R. M.; Thomas, Y.

1977-01-01

311

Nova Scorpii 2012 = MOA 2012 BLG-320  

NASA Astrophysics Data System (ADS)

Nova Scorpii 2012 = MOA 2012 BLG-320 discovered by: R. M. Wagner (Ohio State University (OSU) and Large Binocular Telescope Observatory), S. Dong (Institute for Advanced Study, Princeton), T. Bensby (Lund Observatory), J. Prieto (Princeton University), I. Bond (Massey University), P. Tristram (Mt. John University Observatory), D. Bennett (University of Notre Dame), K. Wada and T. Sumi (Osaka University), and the Microlensing Observations in Astrophysics (MOA) collaboration; A. Gould (OSU), and the Microlensing Follow-Up Network (microFUN) collaboration; and S. Starrfield (Arizona State University) Discovery Magnitude: magnitude I = 18.5 Discovery date: 2012 May 22.80 UT Coordinates: RA 17 50 53.90, Dec -32 37 20.5 (2000.0) Spectra: A high-resolution optical spectrum obtained June 4.08 UT with the Very Large Telescope (+ UVES) indicates the object is most likely a slow Fe II-type nova. Report observations to the AAVSO International Database.

Waagen, Elizabeth O.

2012-06-01

312

DW1029M, a novel botanical drug candidate, inhibits advanced glycation end-product formation, rat lens aldose reductase activity, and TGF-?1 signaling.  

PubMed

DW1029M is a botanical extract consisting of Morus bark and Puerariae radix, produced by Dong-Wha Pharmaceutical, for nephroprotective drug development; it has been in phase II clinical trials in Korea. In our mechanistic investigations, we found that DW1029M inhibits advanced glycation end products (AGEs), rat lens aldose reductase (RLAR), and transforming growth factor (TGF)-?1 signaling, all of which are implicated in diabetic complications such as diabetic nephropathy and diabetic retinopathy. DW1029M inhibits AGE formation via Fe(2+) chelation. The extract contains 13 active constituents that inhibit AGE formation, 8 active constituents that inhibit RLAR activity, and 1 inhibitor of TGF-?1 signaling. Our results suggest DW1029M protects against diabetic nephropathy via blockade of AGE formation, RLAR activity, and TGF-?1 signaling. PMID:24694590

Yoon, Joobyoung; Lee, Hyunyong; Chang, Hwan Bong; Choi, Hyunsik; Kim, Yong Sung; Rho, Yang Kook; Seong, Seungkyoo; Choi, Dong Hwa; Park, Dongeun; Ku, Bonchul

2014-05-15

313

Calculation of ?-decay energies of superheavy nuclei in a hybrid method  

NASA Astrophysics Data System (ADS)

A recent proposed method for ?-decay energies (Q?) [J. M. Dong, W. Zuo, and W. Scheid, Phys. Rev. Lett.PRLTAO0031-900710.1103/PhysRevLett.107.012501 107, 012501 (2011)] can reproduce experimental data of superheavy nuclei (SHN) with an rms value of less than 100 keV. However, a sinusoid-like periodic deviation from experiments, which limits the accuracy in predictions, is observed when using different reference nuclei. In this paper, we have further extended this hybrid method, i.e., to predict Q? of the as-yet-unobserved SHN with the help of known nuclei. It is found that the systematic deviation seen in the previous study is rooted in the nuclear mass model employed. By further analyzing the source of errors, different nuclear mass models are evaluated based on the same procedure.

Li, Z.; Sun, B.; Shen, C. H.; Zuo, W.

2013-11-01

314

Advanced development in chemical analysis of Cordyceps.  

PubMed

Cordyceps sinensis, also called DongChongXiaCao (winter worm summer grass) in Chinese, is a well-known and valued traditional Chinese medicine. In 2006, we wrote a review for discussing the markers and analytical methods in quality control of Cordyceps (J. Pharm. Biomed. Anal. 41 (2006) 1571-1584). Since then this review has been cited by others for more than 60 times, which suggested that scientists have great interest in this special herbal material. Actually, the number of publications related to Cordyceps after 2006 is about 2-fold of that in two decades before 2006 according to the data from Web of Science. Therefore, it is necessary to review and discuss the advanced development in chemical analysis of Cordyceps since then. PMID:23688494

Zhao, J; Xie, J; Wang, L Y; Li, S P

2014-01-01

315

A new Cyrtodactylus (Squamata: Gekkonidae) from Khanh Hoa Province, southern Vietnam.  

PubMed

We describe a new species of the genus Cyrtodactylus from southern Vietnam, based on morphological and molecular datasets. Cyrtodactylus cucdongensis sp. nov. is described on the basis of seven specimens collected from Cuc Dong Cape, Khanh Hoa Province. The new species can be distinguished from the remaining bent-toed geckos by a combination of the following characters: maximum SVL 65.9 mm; 16-19 dorsal tubercle rows; 41-44 ventral scales at midbody; 5 or 6 precloacal pores in males, 4-6 pitted precloacal scales in females; no femoral pores; 6-13 enlarged precloacal scales; 5-9 enlarged femoral scales; no transversally enlarged subcaudals; dorsal pattern consisting of irregular dark bands. This is the 33rd species of Cyrtodactylus known from Vietnam. PMID:24872243

Schneider, Nicole; Phung, Trung My; Le, Minh Duc; Nguyen, Truong Quang; Ziegler, Thomas

2014-01-01

316

Magnetism and magnetic anisotropy of Ni xPd 1-x alloy  

NASA Astrophysics Data System (ADS)

Single-crystalline Ni xPd 1-x thin films with a face-centered cubic structure for the whole stoichiometry 0? x?1 have been achieved on Cu(1 0 0) via molecular beam epitaxy (MBE). The ferromagnetism shows up at x?0.25 at 300 K and the total magnetization decreases as the Pd concentration increases, which confirms our earlier first-principles calculations (Y.S. Shi, M.F. Wang, D. Qian, G.S. Dong, X.F. Jin, D.S. Wang, J. Magn. Magn. Mater. 277 (2004) 71). The magnetocrystalline anisotropy of Ni xPd 1-x is determined to be cubic with a negative K1, as measured by the magneto-optical Kerr effect (MOKE) technique with a rotating magnetic field (ROTMOKE).

Tang, Z. B.; Tian, C. S.; Yin, L. F.; Dong, G. S.; Jin, Xiaofeng

2007-03-01

317

Deep Water Oceanic Wakes: a simple case study  

NASA Astrophysics Data System (ADS)

In present study we investigate the formation and evolution of oceanic wakes generated by obstacle of a real island shape (in our case - Madeira island) but without consideration of bathymetry around it. Numerical simulations using Regional Ocean Modeling System (ROMS) are presented. ROMS is a free-surface, terrain-following, primitive equations ocean model (Shchepetkin and McWilliams, 2005). Numerical models are often used to study the formation and evolution of the leeward, mesoscale and sub-mesoscale flows around of islands (Dietrich et al., 1996; Dong et al., 2007; Heywood et al., 1996). Madeira archipelago is a group of deep-sea islands located at Northeast Atlantic at about 33°N, 17°W. The biggest island of the archipelago is Madeira Island with about 50 km in east-west and 20 km in north-south direction. Its obstruction to the incoming oceanic and atmospheric flows induces leeward wake instabilities. This phenomena is observed using remote sensing and field data (Caldeira et al., 2002). We use the similar methodology to study Madeira island wakes problem as it was presented at Dong et al., 2007. The main difference between their study and ours is that they carried out experiences with an idealized cylindrical obstacle and we are using an island with its real shape at the surface and with vertical sides. The island was centered in a geostrophic channel like configuration with a prescribed surface intensified meridional (southward) inflow at the upstream boundary (i.e., our study is dedicated to the wakes, generated at the eastern and western part of Madeira Island). Eastern and Western channel boundaries were set to slippery-tangential and zero normal conditions, whereas boundaries around the island were set to zero-normal and no-slip flow. A clamped condition with a sponge layer was applied at the southern outflow boundary for outgoing current and density profile. The initial conditions for the entire domain were set equal to the upstream boundary condition expect at the island points. Our numerical simulations were devoted to study of various dynamical flow regimes. Obtained results showed that oceanic wakes formations were sensitive to three dimensionless parameters that representing a ratio between inertial and frictional forces - Reynolds number (Re), rotational effects - Rossby number (Ro) and stratification effects - Burger number (Bu). Wake asymmetries induce different behaviour for cyclonic and anti-cyclonic eddies than that showed by Dong et al., 2007. References: Caldeira, R.M.A., S. Groom, P. Miller, D. Pilgrim and N. Nezlin, 2002: Sea-surface signatures of the island mass effect phenomena around Madeira Island, Northeast Atlantic, Remote Sensing of the Environment, 80, 336-360. Dietrich, D.E., M.J. Bowman, C.A. Lin and A. Mestas-Nunez, 1996: Numerical studies of small island wakes, Geophysics, Astrophysics and Fluid Dynamics, 83, 195-231. Dong, C., J.C. McWilliams and A. Shchepetkin, 2007: Island Wakes in Deep Water, Journal of Physical Oceanography, 37, 962-981. Heywood, K.J., D.P Stevens, G.R. Bigg, 1996: Eddy formation behind the tropical island of Aldabra, Deep-Sea Research I, 43, 555-578. Shchepetkin, A.F., and J.C. McWilliams, 2005: The Regional Ocean Modeling System: A split-explicit, free-surface, topography-following-coordinate oceanic model, Ocean Modelling, 9, 347-404.

Luis, E. A.; Boutov, D.

2009-04-01

318

Investigating Cultural Evolution Using Phylogenetic Analysis: The Origins and Descent of the Southeast Asian Tradition of Warp Ikat Weaving  

PubMed Central

The warp ikat method of making decorated textiles is one of the most geographically widespread in southeast Asia, being used by Austronesian peoples in Indonesia, Malaysia and the Philippines, and Daic peoples on the Asian mainland. In this study a dataset consisting of the decorative characters of 36 of these warp ikat weaving traditions is investigated using Bayesian and Neighbornet techniques, and the results are used to construct a phylogenetic tree and taxonomy for warp ikat weaving in southeast Asia. The results and analysis show that these diverse traditions have a common ancestor amongst neolithic cultures the Asian mainland, and parallels exist between the patterns of textile weaving descent and linguistic phylogeny for the Austronesian group. Ancestral state analysis is used to reconstruct some of the features of the ancestral weaving tradition. The widely held theory that weaving motifs originated in the late Bronze Age Dong-Son culture is shown to be inconsistent with the data.

Buckley, Christopher D.

2012-01-01

319

Global Performing Arts Database  

NSDL National Science Digital Library

The Global Performing Arts Database (GloPAD) is a trove of "detailed, multilingual descriptions of digital images, texts, video clips, sound recordings, and complex media objects related to the performing arts around the world." Currently, the site contains over 4,500 records and was made possible by funds provided by the Institute of Museum and Library Services. New visitors may wish to peruse the About GloPAD area, though detailed search information can be found via the Using GloPAD section. The homepage contains some fine Suggested Searches, including Show Boat, The Government Inspector, and Bernard Shaw. The Production Records area is quite a find, as it contains detailed information for shows as diverse as the 1944 Broadway production of the "On the Town" and the 1996 water puppetry show from the Kim Dong Theatre in Hanoi. [KMG

2006-01-01

320

Investigating cultural evolution using phylogenetic analysis: the origins and descent of the southeast Asian tradition of warp ikat weaving.  

PubMed

The warp ikat method of making decorated textiles is one of the most geographically widespread in southeast Asia, being used by Austronesian peoples in Indonesia, Malaysia and the Philippines, and Daic peoples on the Asian mainland. In this study a dataset consisting of the decorative characters of 36 of these warp ikat weaving traditions is investigated using Bayesian and Neighbornet techniques, and the results are used to construct a phylogenetic tree and taxonomy for warp ikat weaving in southeast Asia. The results and analysis show that these diverse traditions have a common ancestor amongst neolithic cultures the Asian mainland, and parallels exist between the patterns of textile weaving descent and linguistic phylogeny for the Austronesian group. Ancestral state analysis is used to reconstruct some of the features of the ancestral weaving tradition. The widely held theory that weaving motifs originated in the late Bronze Age Dong-Son culture is shown to be inconsistent with the data. PMID:23272211

Buckley, Christopher D

2012-01-01

321

Division X: Radio Astronomy  

NASA Astrophysics Data System (ADS)

The business meeting of Division X in the IAU 2009GA took place in three sessions during the day of August 6, 2009. The meeting, being well attended, started with the approval for the meeting agenda. Then the triennium reports were made in the first session by the president of Division X, Ren-Dong Nan, and by the chairs of three working groups: “Historic Radio Astronomy WG” by Wayne Orchiston, “Astrophysically Important Lines WG” by Masatoshi Ohishi, and “Global VLBI WG” by Tasso Tzioumis (proxy chair appointed by Steven Tingay). Afterwards, a dozen reports from observatories and worldwide significant projects have been presented in the second session. Business meeting of “Interference Mitigation WG” was located in the third session.

Nan, Ren-Dong; Taylor, Russ; Rodriguez, Luis F.; Chapman, Jessica; Dubner, Gloria; Garrett, Michael; Goss, W. Miller; Torrelles, Jose M.; Hirabayashi, Hisashi; Carilli, Chris; Hills, Richard; Shastri, Prajval

2010-05-01

322

Impact of Health Insurance on Health Care Treatment and Cost in Vietnam: A Health Capability Approach to Financial Protection  

PubMed Central

We applied an alternative conceptual framework for analyzing health insurance and financial protection grounded in the health capability paradigm. Through an original survey of 706 households in Dai Dong, Vietnam, we examined the impact of Vietnamese health insurance schemes on inpatient and outpatient health care access, costs, and health outcomes using bivariate and multivariable regression analyses. Insured respondents had lower outpatient and inpatient treatment costs and longer hospital stays but fewer days of missed work or school than the uninsured. Insurance reform reduced household vulnerability to high health care costs through direct reduction of medical costs and indirect reduction of income lost to illness. However, from a normative perspective, out-of-pocket costs are still too high, and accessibility issues persist; a comprehensive insurance package and additional health system reforms are needed.

Nguyen, Kim Thuy; Khuat, Oanh Thi Hai; Pham, Duc Cuong; Khuat, Giang Thi Hong

2012-01-01

323

The North Korean missile program: How advanced is it?  

SciTech Connect

For the past three years there have been increasing numbers of reports that North Korea is developing a 1,000-1,300-kilometer range missile generally referred to as the NoDongg-1. Pyongyang`s missile program has generated international concern because of North Korea`s potential nuclear capabilities, its proximity to South Korea and Japan and its reported missile sales to Iran, Syria and Libya. In June 1993, Japanese and South Korean wire services reported that North Korea had test fired several missiles into the Sea of Japan in late May, at least two of which were though to be NoDong-1 missiles. A missile with a 1,300-kilometer range would give North Korea the capability to reach all of Japan, and give Iran and Libya the capability to reach all of Israel.

Wright, D.; Kadyshev, T.

1994-04-01

324

Magneto-optical contrast in liquid-state optically-detected NMR spectroscopy  

NASA Astrophysics Data System (ADS)

We use optical Faraday rotation (OFR) to probe nuclear spins in real time at high-magnetic field in a range of diamagnetic sample fluids [1]. This technique is shown to speciate functional groups with the same chemical shifts as is seen in conventional NMR, however, the intensities of the OFR-NMR peaks are influenced by optical detuning and hyperfine couplings. We investigate protons at chemically-distinct sites and other lower-gyromagnetic-ratio nuclei including carbon, fluorine and phosphorous [2]. Binary mixtures for protonated systems were also tested and the results suggest that the present approach is sensitive to the solvent-solute dynamics in ways complementary to those known in inductive NMR. [4pt] [1] D. Pagliero, W. Dong, D. Sakellariou and C. A. Meriles. J. Chem. Phys. 133, 154505 (2010). [0pt] [2] D. Pagliero and C. A. Meriles. Proc. Natl. Aca. Sci. USA (2011) in press.

Pagliero, Daniela; Meriles, Carlos

2012-02-01

325

Differential autumn migration of the aquatic warbler Acrocephalus paludicola  

NASA Astrophysics Data System (ADS)

Relatively little attention has been paid to sex differences in the migration of birds in autumn. We studied the autumn migration strategy of molecularly sexed males and females in the globally threatened aquatic warbler Acrocephalus paludicola. We captured 176 birds at a stopover site in the Loire estuary at Donges, France. The median date for the passage of adults was 8 days earlier in males than females, although the timing of migration in first-year males and females was similar. This indicates that males, who are without parental duties, can start their migration earlier than females and first-year birds. Adults were significantly heavier than immature birds but did not have higher fat scores. In both age categories, more males (two to three times more) were captured. However, various factors (including tape-luring) can affect observed sex ratio.

Wojczulanis-Jakubas, Katarzyna; Jakubas, Dariusz; Foucher, Julien; Dziarska-Pa?ac, Joanna; Dugué, Hubert

2013-11-01

326

The anatomy, taphonomy, taxonomy and systematic affinity of Markuelia: Early Cambrian to Early Ordovician scalidophorans  

USGS Publications Warehouse

Markuelia is a vermiform, annulated introvertan animal known as embryonic fossils from the Lower Cambrian to Lower Ordovician. Analysis of an expanded and revised dataset for Introverta shows that the precise position of Markuelia within this clade is dependent on the taxa included. As a result, Markuelia is assigned to the scalidophoran total group to reflect uncertainty as to whether it is a stem-scalidophoran or a stem-priapulid. The taxonomy of the genus is revised to provide an improved taxonomic framework for material assigned to Markuelia. Five species are recognized: M. secunda Val'kov, M. hunanensis Dong and Donoghue, M. lauriei Haug et al., M. spinulifera sp. nov. and M. waloszeki sp. nov. Finally, the preservation of Markuelia is evaluated in the light of both the taphonomy of the fossil embryos themselves and the experimental taphonomy of the priapulid Priapulus caudatus, which has been proposed as both a close relative and an anatomical analogue of Markuelia. ?? The Palaeontological Association.

Dong, X. -P.; Bengtson, S.; Gostling, N. J.; Cunningham, J. A.; Harvey, T. H. P.; Kouchinsky, A.; Val'Kov, A. K.; Repetski, J. E.; Stampanoni, M.; Marone, F.; Donoghue, P. C. J.

2010-01-01

327

Compilation of Published Estimates of Annual Geocenter Motions Using Space Geodesy  

NASA Technical Reports Server (NTRS)

The definition of the term "geocenter motion" depends on the adopted origin of the reference frame. Common reference frames used in Space Geodesy include: the center of mass of the whole Earth (CM), the center of mass of the Solid Earth without mass load (CE), and the center of figure of the outer surface of the Solid Earth (CF). There are two established definitions of the term geocenter: one, the vector offset of CF relative to CM and, two, the reverse, the vector offset of CM relative to CF. Obviously, their amplitude is the same and their phase differs by 180 deg. Following Dong et al. [2003], we label the first X(sub CF, sup CM) and the second X(sup CF, sup CM) (i.e., the superscript represents the frame, the subscript represents any point in the frame).

Elosegui, P.

2005-01-01

328

Improved method of laser photovaporization for endometrium cases  

NASA Astrophysics Data System (ADS)

Endometrium was destroyed by Nd-YAG laser under B ultrasonic monitoring through direct application of a fibrous catheter specially designed by Dong-lin Lee (an optical engineer). Of the 12 patients 11 cases had dysfunctional uterine bleeding and one case had postmenopausal uterine bleeding. The mean time of laser treatment was 5' 45', the mean volume of irrigating solution was 850 ml, and the mean estimated blood loss was less than 9 ml. All patients showed neither evidence of infection nor uterine perforation. Follow-up examination for 3 - 16 months showed amenorrhea in 4 cases, spotting in 4 cases, and obviously reduced menstrual flow in 3 cases. One patient, partly treated, had normal menstruation.

Xia, En-Ju; Lu, Hua; Chen, En-Ling; Wan, Hong-Yue

1995-03-01

329

Distribution of charged nanograins in the Enceladus plume  

NASA Astrophysics Data System (ADS)

Charged nanometer sized grains in the Enceladus south-pole eruptive plume have been detected by the Cassini Plasma Spectrometer (CAPS) during the two encounters E3 and E5 (Jones et al., 2009). A much weaker signal of negatively charged nanograins (but no positively charged ones) was also detected by CAPS during E7 (Hill et al., 2011 MOP conference). We will analyze the observations of the nanograins during these three encounters and compare the nanograin measurements with our previous analysis of the Cassini Ion and Neutral Mass Spectrometer (INMS) measurements of the water vapor plumes (Dong et al., 2011). We will also study the motion and the charging mechanism of the nanograins, and analyze the corresponding results from the upcoming E14 encounter (Oct. 1, 2011).

Dong, Y.; Hill, T. W.

2011-12-01

330

The oldest flora of the South China Block, and the stratigraphic bearings of the plant remains from the Ngoc Vung Series, northern Vietnam  

NASA Astrophysics Data System (ADS)

Several outcrops of the Late Silurian and Devonian of the Ngoc Vung Series, northern Vietnam, yielded plant remains. The Late Silurian localities delivered the earliest known flora of the South China block. Although the fossils are fragmentary, they complement our knowledge about the global composition of the flora. The major components of the flora are plants with dichotomous habit and terminal bivalvate sporangia, which are close relatives to zosterophylls, and zosterophylls. Plants with possible euphyllophyte affinities and bryophytes are occasionally present. This floral composition is similar to that of the rich, younger South China block assemblages from the Posongchong and Xujiachong Formations of China, considered Pragian in age. The South China block flora is therefore likely to have been dominated by zosterophylls and pre-zosterophylls at least from the Late Silurian to the Pragian (i.e. a 20 million years long period). It also strengthens the hypothesis that more derived plants were present on eastern Gondwana earlier that elsewhere, in the first steps of tracheophyte evolution. The Devonian localities of the Ngoc Vung Series delivered a thick fibrous stem fragment and a basal euphyllophyte. These latter plant remains provide some stratigraphic data. The large stem fragment is consistent with an Eifelian age for the Duong Dong Formation (part of the Ngoc Vung Series), as suggested by the brachiopod fauna. The accompanying basal euphyllophyte displays a combination of characters (axes 3-4 mm wide and lateral branchings) that is also consistent with an Eifelian age, but possibly more characteristic of the Emsian flora. It is therefore suggested that the stratigraphic range of the Duong Dong Formation might be extended down to the Emsian.

Gonez, Paul; Nguyên Huu, Hung; Ta Hoa, Phuong; Clément, Gaël; Janvier, Philippe

2012-01-01

331

[Distribution and health risk assessment of heavy metals of groundwaters in the irrigation district of the lower reaches of Yellow River].  

PubMed

In order to investigate the contamination levels of groundwater in the irrigation districts of the lower reaches of Yellow River, fifty-nine groundwater samples collected from the irrigation districts of Henan and Shandong provinces, were analyzed. Health risks associated with eleven metals (Ba, Cd, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Se and Zn) were assessed using water pollution index and USEPA health risk assessment model. The average concentrations of Fe with 0.496 mg x L(-1) and Zn with 0.445 mg x L(-1) were higher than the concentrations of other heavy metals. Concentrations of Fe, Mn, Se and Zn exceeded the relevant standards and the over-standard rates by 27.12%, 27.12%, 15.25% and 5.09%, respectively. Inverse distance weighted method was applied to estimate the unobserved points and their distribution maps were obtained, which indicated that areas of over-standard heavy metals were Wucheng country, Fan country, Dong'e country, Yucheng city and Guan country. Health risks of ingestion of water for all non-carcinogenic metals are higher than those of dermal absorption, while health risks of ingestion of water for carcinogenic metals is lower than those of dermal absorption. Among the health risks caused by the carcinogenic metals in drinking water and dermal absorption, the highest risks associated with Cr, are seven times and twenty-eight times as that of Cd, respectively, but both were significantly lower than the maximum allowance levels recommended by ICRP (5 x 10(-5) a (-1)). The non-carcinogenic metal risks( Ba, Cu, Fe, Mn, Mo, Ni, Pb, Se and Zn), ranging from 1.73 x 10(-13) to 3.46 x 10(-10) a(-1) in dermal absorption and from 1.13 x 10(-9) to 6.06 x 10(-8) a(-1) in drinking water, were much lower than the maximum allowance levels. PMID:23487927

Zhang, Yan; Li, Fa-Dong; Ouyang, Zhu; Zhao, Guang-Shuai; Li, Jing; Liu, Qiang

2013-01-01

332

Reconhecimento de faces humano: Uma modelagem computacional baseada em componentes polares  

Microsoft Academic Search

O sistema visual ? comumente considerado sob uma ?tica Cartesiana, mas v?rios \\u000aestudos nos quais utilizaram-se t?cnicas eletrofisiol?gicas e psicof?sicas levantaram \\u000aevid?ncias de um suposto processamento elementar em coordenadas polares \\u000a[Mahon & De Valois, 2001; Gallant et al. 1993; Gallant et al. 1996; Wilson & Wilkinson, 1998]. \\u000aConsiderando a import?ncia dessa possibilidade, definimos quatro quest?es fundamentais: \\u000a(1) Quais s?o as

Y. Zana; J. P. Mena-Chalco

2006-01-01

333

Teoria das Ondas de Elliott- Uma aplicação ao mercado de ações aa Bm&Fbovespa  

Microsoft Academic Search

Os preços dos ativos negociados em bolsas de valores, assim como qualquer outro tipo de commodity do mercado financeiro, oscilam naturalmente com a procura por esses produtos. Essas oscilações, juntamente com a assimetria das informações acerca dos preços desses produtos geram processos de volatilidade. Charles Dow, no início do século XX criou índices setoriais, nos quais reunia papéis da mesma

Daniele Ferreira de Sousa

334

Development of a Competitive Binding Assay System with Recombinant Estrogen Receptors from Multiple Species  

EPA Science Inventory

ABSTRACT In the current study, we developed a new system using full-length recombinant baculovirus-expressed estrogen receptors which allows for direct comparison of binding across species. Estrogen receptors representing five vertebrate classes were compared: human (hERa), quai...

335

DIFERENCIAÇÃO OU NECESSIDADE NA CERTIFICAÇÃO ISO 9001: UMA ANÁLISE EM DUAS EMPRESAS AGROINDUSTRIAIS  

Microsoft Academic Search

O presente estudo de caso foi realizado em duas organizações agroindustriais, a AGCO do Brasil Comércio e Indústria Ltda. e a Tecno Moageira S\\/A, tratando-se de um estudo de caso de natureza exploratória e descritiva, que objetivou analisar os motivos pelos quais essas organizações implementaram a certificação ISO 9001, em torno da seguinte problemática: Qual o real interesse da certificação

Alexandre Melo Abicht; Alessandra Carla Ceolin; Augusto Faria Correa; Paulo Rodrigo Pereira; Tania Nunes da Silva

2008-01-01

336

O Potencial Distributivo do Imposto de Rendapessoa Física (IRPF)  

Microsoft Academic Search

Este texto argumenta a favor de níveis mais elevados de Imposto de Renda-Pessoa Física (IRPF). Verificamos que, de todos os países para os quais existem informações, o Brasil é o que menos arrecada IRPF relativo à Carga Tributária Bruta (CTB). O IRPF é responsável por algo em torno de 6% da CTB, um pouco mais que 2% do Produto Interno

Sergei Soares; Fernando Gaiger Silveira; Claudio Hamilton dos Santos; Fábio Monteiro Vaz; André Luis Souza

2009-01-01

337

AVALIAÇÃO IN VITRO DA INFILTRAÇÃO MARGINAL EM PERFURAÇÕES DE FURCA UTILIZANDO-SE AGREGADO TRIÓXIDO MINERAL E RESINA COMPOSTA IN VITRO EVALUATION OF THE SEALING IN FURCAL PERFORATION USING MINERAL TRIOXIDE AGGREGATE AND COMPOSITE RESIN  

Microsoft Academic Search

RESUMO Métodos: Avaliou-se in vitro a infiltração marginal em perfurações de furca do Agregado Trióxido Mine- ral (MTA) isolado ou associado à resina composta com ou sem condicionamento ácido e a porcentagem de extrusão dos materiais. Utilizaram-se 36 molares humanos extraídos, os quais foram divididos em três grupos experimentais (n=10): Grupo 1 - MTA, Grupo 2 - MTA + resina

Beatriz Belardinelli; Érico de Mello Lemos; Danilo Minor Shimabuko

338

Estudo do sector eléctrico e electrónico: uma análise desagregada, ao nível dos 40 principais produtos do comércio bilateral comunitário e ao nível das principais empresas, para o período 1980 - 1992  

Microsoft Academic Search

Neste artigo faz-se: I) a caracterização do sector eléctrico - electrónico utilizando vários indicadores, dos quais destacamos as medidas de capital humano, em termos de stock e de fluxo, as medidas de concentração industrial e da escala mínima de eficiência (economias de escala) as medidas do peso das PMEs e as medidas da inovação tecnológica; ÍI) o ranking dos principais

Horácio Crespo Faustino

1995-01-01

339

O “TRATORAÇO”, E A VULNERABILIDADE DO MONOCULTIVO DE GRÃOS NA AGRICULTURA BRASILEIRA  

Microsoft Academic Search

Em junho de 2005 houve em todo o Brasil uma intensa mobilização de produtores rurais a qual culminou com manifestações em praças e estradas nas quais os tratores se faziam presentes ruidosamente, bloqueando o acesso e impedindo o trânsito. Foi intensa a cobertura da imprensa a estas manifestações que levaram a concessões governamentais e mudanças nos instrumentos de política agrícola

Amilcar Baiardi; Januzia Mendes; Maria Lucia Sodre; Elizabeth Maria Ramos; Vivian Liborio Pinto

2008-01-01

340

Os determinantes da desigualdade de renda no Brasil: Luta de classes ou heterogeneidade educacional?  

Microsoft Academic Search

Este trabalho considera a questão: quais os principais determinantes da desigualdade de renda no Brasil? Após lembrar a importância da questão para a nossa economia, o artigo sugere um arcabouço genérico para categorizar os determinantes da distribuição de renda, baseado em cinco grupos de fatores: caraterísticas natas dos indivíduos; suas características adquiridas; o papel do mercado de trabalho; o papel

Francisco de Hollanda Guimarães Ferreira

2000-01-01

341

La violenza sulle donne immigrate in Europa  

Microsoft Academic Search

Este texto caracteriza a situação das mulhres migrantes na União Européia. Na Europa, em 2006, havia um contingente de 18.5 milhões de migrantes procedentes de países do Terceiro Mundo, 54% dos quais eram mulheres. As mulheres migrantes sofrem vulnerabilidades ligadas ao trabalho, participação política e social, maior exposição às violências e sexismo. As autoras apresentam a legislação atual referente à

Anne Van der Troost; Sandra Regina Martini Vial

2008-01-01

342

Levantamento dos Fatores Limitantes da Produtividade Leiteira na Regi- ão de Anápolis-Go  

Microsoft Academic Search

O presente trabalho foi realizado no município de Anápolis-GO no mês de julho de 1995. Consistiu de uma avaliação da atividade pecuária em fazendas leiteiras com o objetivo de, através da análise dos principais índices zootécnicos, determinar quais os fatores limitantes para o aumento da produtividade. Constou da aplicação de um questionário de levantamento sócio-econômico da produção em uma amostra

EDMUNDO BENEDETTI; RENAN RODRIGUES DE CARVALHO

343

De Mestre Valentim a Roberto Burle Marx: los jardines históricos brasileros  

Microsoft Academic Search

A América nasceu sobre o signo do paraíso perdido no imaginário do europeu, navegador e renascentista. O novo território prestou-se como um campo de experimentação, os conquistadores dominaram seus nativos, organizaram cidades e campos, e na dialética das culturas, criou-se o laboratório americano. No clássico confronto entre civilização e natureza, o território brasileiro acomodou peculiares experimentações, as quais valeriam descrever

Hugo Segawa; Guilherme Mazza Dourado

344

Development of the scintillator-based probe for fast-ion losses in the HL-2A tokamak.  

PubMed

A new scintillator-based lost fast-ion probe (SLIP) has been developed and operated in the HL-2A tokamak [L. W. Yan, X. R. Duan, X. T. Ding, J. Q. Dong, Q. W. Yang, Yi Liu, X. L. Zou, D. Q. Liu, W. M. Xuan, L. Y. Chen, J. Rao, X. M. Song, Y. Huang, W. C. Mao, Q. M. Wang, Q. Li, Z. Cao, B. Li, J. Y. Cao, G. J. Lei, J. H. Zhang, X. D. Li, W. Chen, J. Chen, C. H. Cui, Z. Y. Cui, Z. C. Deng, Y. B. Dong, B. B. Feng, Q. D. Gao, X. Y. Han, W. Y. Hong, M. Huang, X. Q. Ji, Z. H. Kang, D. F. Kong, T. Lan, G. S. Li, H. J. Li, Qing Li, W. Li, Y. G. Li, A. D. Liu, Z. T. Liu, C. W. Luo, X. H. Mao, Y. D. Pan, J. F. Peng, Z. B. Shi, S. D. Song, X. Y. Song, H. J. Sun, A. K. Wang, M. X. Wang, Y. Q. Wang, W. W. Xiao, Y. F. Xie, L. H. Yao, D. L. Yu, B. S. Yuan, K. J. Zhao, G. W. Zhong, J. Zhou, J. C. Yan, C. X. Yu, C. H. Pan, Y. Liu, and the HL-2A Team, Nucl. Fusion 51, 094016 (2011)] to measure the losses of neutral beam ions. The design of the probe is based on the concept of the ?-particle detectors on Tokamak Fusion Test Reactor (TFTR) using scintillator plates. The probe is capable of traveling across an equatorial plane port and sweeping the aperture angle rotationally with respect to the axis of the probe shaft by two step motors, in order to optimize the radial position and the collimator angle. The energy and the pitch angle of the lost fast ions can be simultaneously measured if the two-dimensional image of scintillation light intensity due to the impact of the lost fast ions is detected. Measurements of the fast-ion losses using the probe have been performed during HL-2A neutral beam injection discharges. The clear experimental evidence of enhanced losses of beam ions during disruptions has been obtained by means of the SLIP system. A detailed description of the probe system and the first experimental results are reported. PMID:24880364

Zhang, Y P; Liu, Yi; Luo, X B; Isobe, M; Yuan, G L; Liu, Y Q; Hua, Y; Song, X Y; Yang, J W; Li, X; Chen, W; Li, Y; Yan, L W; Song, X M; Yang, Q W; Duan, X R

2014-05-01

345

Possible polyphase metamorphic evolution of high grade metabasic rocks from the Songshugou ophiolite, Qinling orogen, China  

NASA Astrophysics Data System (ADS)

The Proterozoic Songshugou ophiolite consists of a series of ultrabasic and tholeitic metabasic rocks. They were emplaced as a lense shaped body into the southern margin of the Qinling Group. Isotope composition and trace element geochemistry display an E-MORB and T-MORB signature for the mafic rocks (Dong et al., 2008). Within the ophiolite sequence some rudimental fresh peridotites (dunites and harzburgites) within serpentines display low CaO (<0.39 wt.%) and Al2O3 (<0.51 wt.%) as well as high MgO (41-48 wt.%) contents, which can be classified as depleted non-fertile mantle rocks. The metabasic rocks comprise the mineral assemblage garnet, amphibole, symplectitic pyroxenes, ilmenite, apatite, ±zoisite, ±sphene and show a strong retrograde metamorphic overprint. Garnet typically contains many inclusions within the core but are nearly inclusion free at the rim. The cores have sometimes snowball textures indicating initially syndeformative growth. Albite and prehnite were found in central parts of garnet. In the outer portions, pargasitic amphibole, rutile and a bluish amphibole, probably glaukophane were found. Garnet zoning pattern clearly show a discontinous growth seen in an sudden increase in grossular and decrease in almandine components. The symplectitic pyroxenes are of diopsidic composition which enclose typically prehnite and not albite, as common in retrograde eclogitic rocks. Different stages of garnet breakdown to plagioclase and amphibole, from thin plagioclase rims surrounding the garnets to plagioclase rich pseudomorphs, can be observed in different samples. Based on symplectitic pyroxenes a high pressure metamorphic event can be concluded (Zhang, 1999). The garnet breakdown to plagioclase and the symplectites clearly indicate a rapid exhumation phase. The age of the metamorphic event is probably related to the closure of the Shangdan ocean during the early Paleozoic. It is unclear if the garnet rims grew during a later stage of the metamorphic cycle or developed during a separate event. The financial support by Eurasia-Pacific Uninet is gratefully acknowledged. Dong, Y.P., Zhou, M.F., Zhang, G.W., Zhou, D.W., Liu, L., Zhang, Q., 2008. The Grenvillian Songshugou ophiolite in the Qinling Mountains, Central China: implications for the tectonic evolution of the Qinling orogenic belt. Journal of Asian Earth Science 32 (5-6), 325-335. Zhang, Z.J., 1999. Metamorphic evolution of garnet-clinopyroxene-amphibole rocks from the Proterozoic Songshugou mafic-ultramafic complex, Qinling Mountains, central China. The Island Arc, 8, 259-280.

Belic, Maximilian; Hauzenberger, Christoph; Dong, Yunpeng; Chen, Danling

2014-05-01

346

Petrology of metabasic and peridotitic rocks of the Songshugou ophiolite, Qinling orogen, China  

NASA Astrophysics Data System (ADS)

The Proterozoic Songshugou ophiolite outcrops as a rootless nappe which was emplaced into the southern margin of the Qinling Group. It consists mainly of amphibolite facies metamafic and -ultramafic rocks. Trace element geochemistry and isotope composition show that the mafic rocks are mainly E-MORB and T-MORB metabasalts (Dong et al., 2008b). Within the ophiolite sequence, ultramafic rocks consist mainly of peridotites and serpentinites. Particularly, extremely fresh dunites and harzburgites, are found which do not display a conspicuous metamorphic overprint. The low CaO (<0.39 wt.%) and Al2O3 (<0.51 wt.%) as well as high MgO (41-48 wt.%) contents classify them as depleted non-fertile mantle rocks. Chromite is found as disseminated phase but can sometimes form massive chromite bands. The platinumgroup mineral Laurite (RuS2) could be identified as inclusion in chromites. Usually part of Ru is substituted by Os and Ir. The metamafic rocks consist of garnet, amphibole, symplectitic pyroxenes, ilmenite, apatite, ±zoisite, ±sphene and show a strong metamorphic overprint. Garnet contains numerous inclusions in the core but are nearly inclusion free at the rim. The cores have sometimes snowball textures indicating initially syndeformative growth. Pure albite and prehnite were found in the central parts of the garnets. In the outer portions, pargasitic amphibole, rutile and rarely glaukophane were found. The symplectitic pyroxenes are of diopsidic composition which enclose prehnite and not albite, as common in retrograde eclogitic rocks. Different stages of garnet breakdown to plagioclase and amphibole, from thin plagioclase rims surrounding the garnets to plagioclase rich pseudomorphs, can be observed in different samples. Based on the glaukophane inclusions and symplectitic pyroxenes a high pressure metamorphic event can be concluded. The garnet breakdown to plagioclase and the symplectites clearly indicate a rapid exhumation phase. The age of the metamorphic event is unclear but probably related to the closure of the Shangdan ocean during the early Paleozoic. The financial support by Eurasia-Pacific Uninet is gratefully acknowledged. Dong, Y.P., Zhou, M.F., Zhang, G.W., Zhou, D.W., Liu, L., Zhang, Q., 2008. The Grenvillian Songshugou ophiolite in the Qinling Mountains, Central China: implications for the tectonic evolution of the Qinling orogenic belt. Journal of Asian Earth Science 32 (5-6), 325-335.

Belic, Maximilian; Hauzenberger, Christoph; Dong, Yunpeng

2013-04-01

347

Development of the scintillator-based probe for fast-ion losses in the HL-2A tokamak  

NASA Astrophysics Data System (ADS)

A new scintillator-based lost fast-ion probe (SLIP) has been developed and operated in the HL-2A tokamak [L. W. Yan, X. R. Duan, X. T. Ding, J. Q. Dong, Q. W. Yang, Yi Liu, X. L. Zou, D. Q. Liu, W. M. Xuan, L. Y. Chen, J. Rao, X. M. Song, Y. Huang, W. C. Mao, Q. M. Wang, Q. Li, Z. Cao, B. Li, J. Y. Cao, G. J. Lei, J. H. Zhang, X. D. Li, W. Chen, J. Chen, C. H. Cui, Z. Y. Cui, Z. C. Deng, Y. B. Dong, B. B. Feng, Q. D. Gao, X. Y. Han, W. Y. Hong, M. Huang, X. Q. Ji, Z. H. Kang, D. F. Kong, T. Lan, G. S. Li, H. J. Li, Qing Li, W. Li, Y. G. Li, A. D. Liu, Z. T. Liu, C. W. Luo, X. H. Mao, Y. D. Pan, J. F. Peng, Z. B. Shi, S. D. Song, X. Y. Song, H. J. Sun, A. K. Wang, M. X. Wang, Y. Q. Wang, W. W. Xiao, Y. F. Xie, L. H. Yao, D. L. Yu, B. S. Yuan, K. J. Zhao, G. W. Zhong, J. Zhou, J. C. Yan, C. X. Yu, C. H. Pan, Y. Liu, and the HL-2A Team, Nucl. Fusion 51, 094016 (2011)] to measure the losses of neutral beam ions. The design of the probe is based on the concept of the ?-particle detectors on Tokamak Fusion Test Reactor (TFTR) using scintillator plates. The probe is capable of traveling across an equatorial plane port and sweeping the aperture angle rotationally with respect to the axis of the probe shaft by two step motors, in order to optimize the radial position and the collimator angle. The energy and the pitch angle of the lost fast ions can be simultaneously measured if the two-dimensional image of scintillation light intensity due to the impact of the lost fast ions is detected. Measurements of the fast-ion losses using the probe have been performed during HL-2A neutral beam injection discharges. The clear experimental evidence of enhanced losses of beam ions during disruptions has been obtained by means of the SLIP system. A detailed description of the probe system and the first experimental results are reported.

Zhang, Y. P.; Liu, Yi; Luo, X. B.; Isobe, M.; Yuan, G. L.; Liu, Y. Q.; Hua, Y.; Song, X. Y.; Yang, J. W.; Li, X.; Chen, W.; Li, Y.; Yan, L. W.; Song, X. M.; Yang, Q. W.; Duan, X. R.

2014-05-01

348

Immunomodulatory and Antidiabetic Effects of a New Herbal Preparation (HemoHIM) on Streptozotocin-Induced Diabetic Mice  

PubMed Central

HemoHIM (a new herbal preparation of three edible herbs: Angelica gigas Nakai, Cnidium officinale Makino, and Paeonia japonica Miyabe) was developed to protect immune, hematopoietic, and self-renewal tissues against radiation. This study determined whether or not HemoHIM could alter hyperglycemia and the immune response in diabetic mice. Both nondiabetic and diabetic mice were orally administered HemoHIM (100?mg/kg) once a day for 4 weeks. Diabetes was induced by single injection of streptozotocin (STZ, 200?mg/kg, i.p.). In diabetic mice, HemoHIM effectively improved hyperglycemia and glucose tolerance compared to the diabetic control group as well as elevated plasma insulin levels with preservation of insulin staining in pancreatic ?-cells. HemoHIM treatment restored thymus weight, white blood cells, lymphocyte numbers, and splenic lymphocyte populations (CD4+ T and CD8+ T), which were reduced in diabetic mice, as well as IFN-? production in response to Con A stimulation. These results indicate that HemoHIM may have potential as a glucose-lowering and immunomodulatory agent by enhancing the immune function of pancreatic ?-cells in STZ-induced diabetic mice.

Kim, Jong-Jin; Choi, Jina; Lee, Mi-Kyung; Kang, Kyung-Yun; Paik, Man-Jeong; Jo, Sung-Kee; Jung, Uhee; Park, Hae-Ran; Yee, Sung-Tae

2014-01-01

349

Snails and trematode infection after Indian Ocean tsunami in Phang-Nga Province, southern Thailand.  

PubMed

The tsunami and non-tsunami affected areas of Takua Pa District, Phang-Nga Province were investigated for fresh- and brackish-water snails that transmit human parasitic diseases during 2006 and 2007. Among 46 snail species found, 17 species of 8 families were freshwater snails, 28 species of another 7 families were brackish-water snails, and 1 species was a land snail. Of these species, 11 freshwater snails, 4 brackish-water snails and 1 land snail were of medical importance. The fresh-water snails were Pomacea canaliculata, Pila angelica, P. gracilis, P. polita, Filopaludina (S.) martensi, F. (F.) s. polygramma, Melanoides tuberculata, Indoplanorbis exuxtus, Radix rubiginosa, Helicorbis umbilicalis, Gyraulus convexiusculus. Four brackish-water snails were Cerithidea cingulata, C. djadjarensis, C. alata, Sermyla riqueti and Achatina fulica was the land snail. I. exutus, M. tuberculata and F. (F.) s. polygramma harbored Xiphidio, Microcercus, Furocercus, Echinostome cercariae, and cercaria without eyespots or tail with hair. Three species of brackish-water snails, Cerithidia cingulata, C. djadjariensis, and C. alata presented with 6 types of trematode cercariae and rediae. Knowledge of medically important snails and their parasitic diseases, and prevention were given to Takua Pa people by poster, pamphlets and broadcasting through community radio. PMID:20578482

Sri-Aroon, Pusadee; Chusongsang, Phiraphol; Chusongsang, Yupa; Pornpimol, Surinthwong; Butraporn, Piyarat; Lohachit, Chantima

2010-01-01

350

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by (1)H-NMR Metabolic Profiling.  

PubMed

Danggui Buxue Tang (DBT) is one of the simplest traditional Chinese medicine (TCM) decoctions, first described in China in 1247 AD. DBT is composed of 2 herbs, Astragali Radix (AR) and Angelica Sinensis Radix (ASR), boiled together in a 5?:?1 ratio. Clinically, DBT is prescribed to women as a remedy for menopausal symptoms. Here, H-NMR metabolic profiling was conducted for DBT and the water extracts of AR or ASR, to evaluate the potential of this chemical profiling method for quality control of the herbal decoction. Principal component analysis (PCA) showed that DBT could be readily distinguished from the water extracts of its constituent herbs by the metabolic profiles. More interestingly, the metabolic profile of DBT was not a simple sum of that of AR and ASR. Asparagine was found at significantly higher concentration in DBT than that in either AR or ASR extract, contributing mainly to the discrimination of DBT sample. In addition, we employed the same method to profile a commercial DBT powder, verifying its authenticity as compared to our prepared DBT. This study is the first to employ H-NMR metabolic profiling for the quality control of traditional Chinese medicine decoctions. PMID:24826194

Chan, Pui Hei; Zhang, Wendy L; Cheung, Chi Yuen; Tsim, Karl W K; Lam, Henry

2014-01-01

351

Hypothesis of active components in volatile oil from a Chinese herb formulation, 'Shao-Fu-Zhu-Yu decoction', using GC-MS and chemometrics.  

PubMed

Traditional Chinese medicine (TCM) with few or no side effects has increasingly attracted attention all over the world. However, the bioactive components and the therapeutic mechanisms are usually not understood because of the complex chemical compositions of these medicines. In this paper, GC-MS coupled with a chemometric method was developed for analysis of active components in volatile oil from a Chinese herb formulation, "Shao-Fu-Zhu-Yu Decoction". The volatile oils, obtained by hydrodistillation from "Shao-Fu-Zhu-Yu Decoction" (SFZYD) and its constituent herbs with abundant volatile oil (Angelica sinensis, Ligusticum chuanxiong, Cinnamomum cassia, Foeniculum vulgare, Zingiber officinale), were chemically analyzed using GC-MS and bioassayed using oxytocin-induced uterine contraction assay in vitro. Then, a mathematic model relating the chemical compositions and their activities in inhibiting mice uterine contraction was established for hypothesis of the bioactive compounds based on chemometrics. As a result, nine compounds which might contribute to the inhibition of oxytocin-induced uterine contraction were selected, and the activities of some of them were further confirmed by our experiments and/or the literature. The data suggest that the developed method is helpful for screening bioactive components from complex mixtures, such as the extracts of TCM. PMID:18338402

Su, Shulan; Hua, Yongqing; Duan, Jin-Ao; Shang, Erxin; Tang, Yuping; Bao, Xingjie; Lu, Yin; Ding, Anwei

2008-04-01

352

New anticancer agents: in vitro and in vivo evaluation of the antitumor and antimetastatic actions of various compounds isolated from medicinal plants.  

PubMed

In this review, in the search for the development of new anticancer drugs, the effects of compounds isolated from various medicinal plants on tumor growth and metastasis, using mice bearing a highly metastatic drug-resistant mouse tumor, were studied. The antitumor and antimetastatic actions of stilbene derivatives isolated from Polygonum and Cassia species were examined. Among the stilbene derivatives, resveratrol and cassiagrol A (stilbene dimer) displayed antitumor and antimetastatic actions through the inhibition of tumor-induced neovascularization in in vitro and in vivo models. It was found that two chalcone derivatives from Angelica keiskei roots also inhibited tumor growth and metastasis in tumor-bearing mice through the inhibition of tumor-induced neovascularization and/or the inhibition of immune suppression caused by tumors. Recently, basidiomycete fungi have been used for the treatment of cancer. Then, the low molecular weight substances were isolated from Agaricus blazei and Ganoderma lucidum as antitumor and antimetastatic substances. It is suggested that these substances of basidiomycete also inhibited tumor growth and metastasis to the lung through the inhibition of tumor-induced neovascularization and/or the inhibition of immune suppression caused by tumors. PMID:15796155

Kimura, Yoshiyuki

2005-01-01

353

The Chinese Herbal Decoction Danggui Buxue Tang Inhibits Angiogenesis in a Rat Model of Liver Fibrosis  

PubMed Central

In this study, we investigated the anti-angiogenic effect of the Chinese herbal decoction Danggui Buxue Tang (DBT; Radix Astragali and Radix Angelicae sinensis in 5?:?1 ratio) in a rat model of liver fibrosis, in order to elucidate its mechanisms of action against liver fibrosis. Liver fibrosis was induced with CCl4 and high-fat food for 6 weeks, and the rats were treated with oral doses of DBT (6?g raw herbs/kg/d) and N-Acetyl-L-cysteine (NAC; 0.1?g/kg/d). The results showed that both DBT and NAC attenuated liver fibrosis and neo-angiogenesis. Furthermore, DBT and NAC improved SOD activity but decreased MDA content and 8-OH-dG in fibrotic livers, with DBT being more effective than NAC. DBT decreased the expression of VEGF, Ang1 and TGF-?1 and their signaling mediators, whereas NAC had no effect on VEGF and VEGFR2 expression. Both DBT and NAC reduced HIF-1? gene and protein expression in fibrotic livers, with DBT being more effective. These data clearly demonstrate that the anti-fibrotic properties of DBT are related to its ability to inhibit angiogenesis and its anti-angiogenic mechanisms are associated with improving oxidative stress, regulating the expression and signaling of angiogenic factors, and especially modulating HIF-1? in fibrotic livers.

Lv, Jing; Zhao, Zhimin; Chen, Yuan; Wang, Qinglan; Tao, Yanyan; Yang, Li; Fan, Tai-Ping; Liu, Chenghai

2012-01-01

354

Decursin and Doxorubicin Are in Synergy for the Induction of Apoptosis via STAT3 and/or mTOR Pathways in Human Multiple Myeloma Cells  

PubMed Central

Background. Combination cancer therapy is one of the attractive approaches to overcome drug resistance of cancer cells. In the present study, we investigated the synergistic effect of decursin from Angelica gigas and doxorubicin on the induction of apoptosis in three human multiple myeloma cells. Methodology/Principal Findings. Combined treatment of decursin and doxorubicin significantly exerted significant cytotoxicity compared to doxorubicin or decursin in U266, RPMI8226, and MM.1S cells. Furthermore, the combination treatment enhanced the activation of caspase-9 and -3, the cleavage of PARP, and the sub G1 population compared to either drug alone in three multiple myeloma cells. In addition, the combined treatment downregulated the phosphorylation of mTOR and its downstream S6K1 and activated the phosphorylation of ERK in three multiple myeloma cells. Furthermore, the combined treatment reduced mitochondrial membrane potential, suppressed the phosphorylation of JAK2, STAT3, and Src, activated SHP-2, and attenuated the expression of cyclind-D1 and survivin in U266 cells. Conversely, tyrosine phosphatase inhibitor pervanadate reversed STAT3 inactivation and also PARP cleavage and caspase-3 activation induced by combined treatment of doxorubicin and decursin in U266 cells. Conclusions/Significance. Overall, the combination treatment of decursin and doxorubicin can enhance apoptotic activity via mTOR and/or STAT3 signaling pathway in multiple myeloma cells.

Jang, Jinsil; Jeong, Soo-Jin; Kwon, Hee-Young; Jung, Ji Hoon; Sohn, Eun Jung; Lee, Hyo-Jung; Kim, Ji-Hyun; Kim, Sun-Hee; Kim, Jin Hyoung; Kim, Sung-Hoon

2013-01-01

355

The Dynamic Nature of the Ligustilide Complex  

PubMed Central

Monomeric phthalides like Z-ligustilide (1) and Z-butylidenephthalide (2) are major constituents of medicinal plants of the Apiaceae family. While 1 has been associated with a variety of observed biological effects, it is also known for its instability and rapid chemical degradation. For the purpose of isolating pure 1 and 2, a gentle and rapid 2-step countercurrent isolation procedure was developed. From a supercritical CO2 fluid extract of Angelica sinensis roots, the phthalides were isolated with high GC-MS purities of 99.4 % for 1 and 98.9 % for 2, and consistently lower qHNMR purities of 98.1 % and 96.4 %, respectively. Taking advantage of molarity-based qHNMR methodology, a time-resolved study of the dynamic changes and residual complexity of pure 1 was conducted. GC-MS and (qH)NMR analysis of artificially degraded 1 provided evidence for the phthalide degradation pathways and optimized storing conditions. Parallel qHNMR analysis led to the recognition of variations in time- and process-dependant sample purity, and has impact on the overall assessment of time dependent changes in complex natural products systems. The study underscores the importance of independent quantitative monitoring as a prerequisite for the biological evaluation of labile natural products such as monomeric phthalides.

Schinkovitz, Andreas; Pro, Samuel M.; Main, Matthew; Chen, Shao-Nong; Jaki, Birgit U.; Lankin, David C.; Pauli, Guido F.

2008-01-01

356

Treatment of Rheumatoid Arthritis with Traditional Chinese Medicine  

PubMed Central

Rheumatoid arthritis (RA) is a chronic inflammatory disease that will affect quality of life and, working efficiency, and produce negative thoughts for patients. Current therapy of RA is treated with disease-modifying antirheumatic drugs (DMARDs). Although most of these treatment methods are effective, most patients still have a pleasant experience either due to poor efficacy or side effects or both. Interleukin-6 receptor (IL6R) is important in the pathogenesis of RA. In this study, we would like to detect the potential candidates which inhibit IL6R against RA from traditional Chinese medicine (TCM). We use TCM compounds from the TCM Database@Taiwan for virtually screening the potential IL6R inhibitors. The TCM candidate compound, calycosin, has potent binding affinity with IL6R protein. The molecular dynamics simulation was employed to validate the stability of interaction in the protein complex with calycosin. The analysis indicates that protein complex with calycosin is more stable. In addition, calycosin is known to be one of the components of Angelica sinensis, which has been indicated to have an important role in the treatment of rheumatoid arthritis. Therefore, calycosin is a potential candidate as lead compounds for further study in drug development process with IL6R protein against rheumatoid arthritis.

Lee, Wen-Yuan; Chen, Hsin-Yi

2014-01-01

357

Multidimensional liquid chromatography system with an innovative solvent evaporation interface.  

PubMed

An orthogonal two-dimensional liquid chromatographic (2D-LC) system was developed by using a vacuum-evaporation loop-type valve interface. Normal-phase liquid chromatography (NPLC) with a bonded CN phase column was used as the first dimension, and reversed-phase liquid chromatography (RPLC) with a C(18) column was used as the second dimension. All the solvents in the loop of the interface were evaporated at 90 degrees C under vacuum conditions, leaving the analytes on the inner wall of the loop. The mobile phase of the second dimension dissolved the analytes in the loop and injected them onto the secondary column, allowing an on-line solvent exchange of a selected fraction from the first dimension to the second dimension. The chromatographic resolution of analytes on the two dimensions was maintained at their optimal condition. Sample loss due to evaporation in the interface was observed that depended on the boiling point of the compound. Separation of sixteen polycyclic aromatic hydrocarbon mixtures and a traditional Chinese medicine Angelica dahurica was demonstrated. PMID:17084846

Tian, Hongzhe; Xu, Jing; Xu, Yuan; Guan, Yafeng

2006-12-22

358

Polyphenols and neuroprotection against ischemia and neurodegeneration.  

PubMed

Neuroprotection of polyphenols in medical plants is getting attention in the world. Scutellaria baicalensis, paeonia veitchii and paeonia suffruticosa have been extensively studied in the last 10 years and show multi-function. They are neuroprotectants, antioxidants, anti-inflammatory and antithrombic agents as well as vasoconstriction inhibitors and amyloid-peptide (A?) cleaners by means of their polyphenols: baicalin, baicalein, wogonin (in scutellaria), and paeonol, paeonoside, paeoniflorin (PF) and 1, 2, 3, 4, 6-Penta-O-galloyl-beta-D-glucose (PGG) (in paeonia veitchii and paeonia suffruticosa). Other 4 medical plants: astragali, ligusticum wallichii, angelica sinensis and carthamus tinctorius (saffron) have been the major medicines to treat ischemia for hundreds of years in China, Korea and Japan. Our recent experimental studies demonstrated the neuroprotective efficacy of the combination of these phyotmedicines on mitigating brain infarction and global ischemia as well as preventing the neurodegeneration following ischemia. Owing to their multi-function, including improving cerebral blood circulation, they therefore have the potential to alleviate the symptoms of degenerative diseases, Alzheimer's disease (AD) and Parkinson's disease (PD). Pharmacology of the 7 herbs and their major relative polyphenols is depicted in the article. PMID:22070681

Lin, B

2011-12-01

359

Optimizing Prescription of Chinese Herbal Medicine for Unstable Angina Based on Partially Observable Markov Decision Process  

PubMed Central

Objective. Initial optimized prescription of Chinese herb medicine for unstable angina (UA). Methods. Based on partially observable Markov decision process model (POMDP), we choose hospitalized patients of 3 syndrome elements, such as qi deficiency, blood stasis, and turbid phlegm for the data mining, analysis, and objective evaluation of the diagnosis and treatment of UA at a deep level in order to optimize the prescription of Chinese herb medicine for UA. Results. The recommended treatment options of UA for qi deficiency, blood stasis, and phlegm syndrome patients were as follows: Milkvetch Root?+?Tangshen?+?Indian Bread?+?Largehead Atractylodes Rhizome (ADR = 0.96630); Danshen Root?+?Chinese Angelica?+?Safflower?+?Red Peony Root?+?Szechwan Lovage Rhizome Orange Fruit (ADR = 0.76); Snakegourd Fruit?+?Longstamen Onion Bulb?+?Pinellia Tuber?+?Dried Tangerine peel?+?Largehead Atractylodes Rhizome?+?Platycodon Root (ADR = 0.658568). Conclusion. This study initially optimized prescriptions for UA based on POMDP, which can be used as a reference for further development of UA prescription in Chinese herb medicine.

Feng, Yan; Qiu, Yu; Zhou, Xuezhong; Wang, Yixin; Xu, Hao; Liu, Baoyan

2013-01-01

360

A chinese herbal decoction, danggui buxue tang, stimulates proliferation, differentiation and gene expression of cultured osteosarcoma cells: genomic approach to reveal specific gene activation.  

PubMed

Danggui Buxue Tang (DBT), a Chinese herbal decoction used to treat ailments in women, contains Radix Astragali (Huangqi; RA) and Radix Angelicae Sinensis (Danggui; RAS). When DBT was applied onto cultured MG-63 cells, an increase of cell proliferation and differentiation of MG-63 cell were revealed: both of these effects were significantly higher in DBT than RA or RAS extract. To search for the biological markers that are specifically regulated by DBT, DNA microarray was used to reveal the gene expression profiling of DBT in MG-63 cells as compared to that of RA- or RAS-treated cells. Amongst 883 DBT-regulated genes, 403 of them are specifically regulated by DBT treatment, including CCL-2, CCL-7, CCL-8, and galectin-9. The signaling cascade of this DBT-regulated gene expression was also elucidated in cultured MG-63 cells. The current results reveal the potential usage of this herbal decoction in treating osteoporosis and suggest the uniqueness of Chinese herbal decoction that requires a well-defined formulation. The DBT-regulated genes in the culture could serve as biological responsive markers for quality assurance of the herbal preparation. PMID:19131392

Choi, Roy C Y; Gao, Qiu T; Cheung, Anna W H; Zhu, Judy T T; Lau, Faye T C; Li, Jun; Li, Winnie Z M; Chu, Glanice K Y; Duan, Ran; Cheung, Jerry K H; Ding, An W; Zhao, Kui J; Dong, Tina T X; Tsim, Karl W K

2011-01-01

361

HemoHIM ameliorates the persistent down-regulation of Th1-like immune responses in fractionated ?-irradiated mice by modulating the IL-12p70-STAT4 signaling pathway.  

PubMed

Whole body irradiated mice appear to experience a down-regulation of the helper T (Th)1-like immune response, and maintain a persistent immunological imbalance. In the current study, we evaluated the effect of HemoHIM (an herbal product made from Angelica Radix, Cnidium officinale , and Paeonia japonica cultivated in Korea) to ameliorate the immunological imbalance induce in fractionated ?-irradiated mice. The mice were exposed to ? rays twice a week (0.5 Gy fractions) for a total dose of 5 Gy, and HemoHIM was administrated orally from 1 week before the first irradiation to 1 week before the final analysis. All experiments were performed 4 and 6 months after their first exposure. HemoHIM ameliorated the Th1- and Th2-related immune responses normally occur in irradiated mice with or without dinitrophenylated keyhole limpet hemocyanin immunization. HemoHIM also restored the natural killer cell activities without changing the percentage of natural killer cells in irradiated mice. Furthermore, the administration of HemoHIM prevented the reduction in levels of interleukin-12p70 in irradiated mice. Finally, we found that HemoHIM enhanced the phosphorylation of signal transducer and activator of transcription (STAT) 4 that was reduced in irradiated mice. Our findings suggest that HemoHIM ameliorates the persistent down-regulation of Th1-like immune responses by modulating the IL-12p70/pSTAT4 signaling pathway. PMID:22439601

Park, Hae-Ran; Jo, Sung-Kee; Choi, Nam-Hee; Jung, Uhee

2012-05-01

362

Restoration of the immune functions in aged mice by supplementation with a new herbal composition, HemoHIM.  

PubMed

The effect of a new herbal composition, HemoHIM, on immune functions was examined in aged mice, in which various immune responses had been impaired. The composition HemoHIM was prepared by adding the ethanol-insoluble fraction to the total water extract of a mixture of three edible herbs, Angelica Radix, Cnidium Rhizoma and Paeonia Radix. Supplementation to the aged mice with HemoHIM restored the proliferative response and cytokine production of splenocytes with a response to ConA. Also, HemoHIM recovered the NK cell activity which had been impaired in the aged mice. Meanwhile aging is known to reduce the Th1-like function, but not the Th2-like function, resulting in a Th1/Th2 imbalance. HemoHIM restored the Th1/Th2 balance in the aged mice through enhanced IFN-gamma and IgG2a production, and conversely a reduced IL-4 and IgG1 production. It was found that one factor for the Th1/Th2 imbalance in the aged mice was a lower production of IL-12p70. However, HemoHIM restored the IL-12p70 production in the aged mice. These results suggested that HemoHIM was effective for the restoration of impaired immune functions of the aged mice and therefore could be a good recommendation for immune restoration in elderly humans. PMID:17705143

Park, Hae-Ran; Jo, Sung-Kee; Jung, Uhee; Yee, Sung-Tae

2008-01-01

363

Preventative Effect of an Herbal Preparation (HemoHIM) on Development of Airway Inflammation in Mice via Modulation of Th1/2 Cells Differentiation  

PubMed Central

HemoHIM, an herbal preparation of three edible herbs (Angelica gigas Nakai, Cnidium officinale Makino, Paeonia japonica Miyabe) is known to increase the Th1 immune response as well as reduce the allergic response in human mast cells. Here, our goal was to determine whether or not HemoHIM could induce Th1 cell differentiation as well as inhibit the development of airway inflammation. To study Th1/Th2 cell differentiation, naive CD4+ T cells isolated from C57BL/6 mouse spleens were cultured with or without HemoHIM. To examine airway inflammation, C57BL/6 mice were fed HemoHIM for 4 weeks before sensitization and provocation with ovalbumin (OVA). In an in vitro experiment, naive CD4+ T cells displayed increased Th1 (IFN-?+ cell) as well as decreased Th2 (IL-4+ cell) differentiation in a HemoHIM concentration-dependent manner. Furthermore, in an airway inflammation mice model, eosinophil numbers in BALF, serum levels of OVA-specific IgE and IgG1, and cytokine (IL-4, IL-5, and IL-13) levels in BALF and the supernatant of splenocytes all decreased upon HemoHIM (100 mg/kg body weight) pretreatment (4 weeks). These results show that HemoHIM attenuated allergic airway inflammation in the mouse model through regulation of the Th1/Th2 balance.

Kim, Jong-Jin; Cho, Hyun Wook; Park, Hae-Ran; Jung, Uhee; Jo, Sung-Kee; Yee, Sung-Tae

2013-01-01

364

Preventative effect of an herbal preparation (HemoHIM) on development of airway inflammation in mice via modulation of Th1/2 cells differentiation.  

PubMed

HemoHIM, an herbal preparation of three edible herbs (Angelica gigas Nakai, Cnidium officinale Makino, Paeonia japonica Miyabe) is known to increase the Th1 immune response as well as reduce the allergic response in human mast cells. Here, our goal was to determine whether or not HemoHIM could induce Th1 cell differentiation as well as inhibit the development of airway inflammation. To study Th1/Th2 cell differentiation, naive CD4(+) T cells isolated from C57BL/6 mouse spleens were cultured with or without HemoHIM. To examine airway inflammation, C57BL/6 mice were fed HemoHIM for 4 weeks before sensitization and provocation with ovalbumin (OVA). In an in vitro experiment, naive CD4(+) T cells displayed increased Th1 (IFN-?(+) cell) as well as decreased Th2 (IL-4(+) cell) differentiation in a HemoHIM concentration-dependent manner. Furthermore, in an airway inflammation mice model, eosinophil numbers in BALF, serum levels of OVA-specific IgE and IgG1, and cytokine (IL-4, IL-5, and IL-13) levels in BALF and the supernatant of splenocytes all decreased upon HemoHIM (100 mg/kg body weight) pretreatment (4 weeks). These results show that HemoHIM attenuated allergic airway inflammation in the mouse model through regulation of the Th1/Th2 balance. PMID:23844220

Kim, Jong-Jin; Cho, Hyun Wook; Park, Hae-Ran; Jung, Uhee; Jo, Sung-Kee; Yee, Sung-Tae

2013-01-01

365

HemoHIM enhances the therapeutic efficacy of ionizing radiation treatment in tumor-bearing mice.  

PubMed

Although radiotherapy is commonly used for a variety of cancers, radiotherapy alone does not achieve a satisfactory therapeutic outcome. In this study, we examined the possibility that HemoHIM can enhance the anticancer effects of ionizing radiation (IR) in melanoma-bearing mice. The HemoHIM was prepared by adding the ethanol-insoluble fraction to the total water extract of a mixture of three edible herbs-Angelica Radix, Cnidium Rhizoma, and Paeonia Radix. Anticancer effects of HemoHIM were evaluated in melanoma-bearing mice exposed to IR. IR treatment (5 Gy at 7 days after melanoma cell injection) reduced the weight of the solid tumors, and HemoHIM supplementation with IR enhanced the decreases in tumor weight (P < .03). In the melanoma-bearing mice treated with IR, HemoHIM administration also increased the activity of natural killer cells and cytotoxic T cells, although the proportions of these cells in spleen were not different. In addition, HemoHIM administration increased the interleukin-2 and tumor necrosis factor-alpha secretion from lymphocytes stimulated with concanavalin A, which seemed to contribute to the enhanced efficacy of HemoHIM in tumor-bearing mice treated with IR. In conclusion, HemoHIM may be a beneficial supplement during radiotherapy for enhancing the antitumor efficacy. PMID:20136435

Park, Hae-Ran; Ju, Eun-Jin; Jo, Sung-Kee; Jung, Uhee; Kim, Sung-Ho

2010-02-01

366

Using Complementary and Alternative Medicines to Target the Host Response during Severe Influenza  

PubMed Central

It is now accepted that an overwhelming inflammatory response is the cause of human deaths from avian H5N1 influenza infection. With this in mind we sought to examine the literature for examples of complementary and alternative medicines that reduce inflammation, and to place the results of this search in the context of our own work in a mouse model of influenza disease, using a pharmaceutical agent with anti-inflammatory properties. Two Chinese herbs, Angelica sinensis (Dang Gui) and Salvia miltiorrhiza (Danshen), have been recently shown to protect mice during lethal experimental sepsis via inhibition of the novel inflammatory cytokine High Mobility Group Box 1 protein (HMGB1). Biochanin A, a ligand of the peroxisome proliferator activated receptors (PPAR) alpha and gamma and the active isoflavone in Trifolium pratense (red clover), has anti-inflammatory properties, and thus could be used as an influenza treatment. This is of great interest since we have recently shown that gemfibrozil, a drug used to treat hyperlipidemia in humans and a synthetic ligand of PPAR alpha, significantly reduces the mortality associated with influenza infections in mice. The inflammation-modulating abilities of these natural agents should be considered in light of what is now known about the mechanisms of fatal influenza, and tested as potential candidates for influenza treatments in their own right, or as adjunct treatments to antivirals.

Cai, Charles; Clark, Ian A.

2010-01-01

367

Simultaneous analysis of nine components in patch preparations of Ru-Yi-Jin-Huang-San by high-performance liquid chromatography.  

PubMed

A facile high-performance liquid chromatography (HPLC) method for the resolution and quantitative measurement of nine marker substances, the active ingredients in patch preparations of Ru-Yi-Jin-Huang-San, was established using gradient elution in the reversed-phase mode. These marker substances included berberine (Phellodendri Cortex), curcumin (Curcumae Rhizoma), imperatorin (Angelicae Dahuricae Radix), magnolol (Magnoliae Cortex), hesperidin (Citri Leiocarpae Exocarpium), glycyrrhizin (Glycyrrhizae Radix), and emodin, sennoside A, sennoside B (Rhei Rhizoma). The ingredients in the water-based and oil-based patches of the formula from different manufactures were also analyzed for quality evaluation. Extracted samples were analyzed by HPLC using a reversed-phase column (Inertsil 5 ODS-2, 4.6-mm I.D. x 250 mm) at 30 degrees C and eluted with a mixture of 20 and 70% acetonitrile aqueous solution in gradient manner at a flow rate of 1.0 ml/min. The detection wavelength varied with time as follows: 275 nm, 0-72 min; 250 nm, 72-105 min; 220 nm, 105-145 min. Relative coefficients of variations of intra- and interday analysis were less than 5%. All the recoveries were 93.30-113.63%. This method could be applied for the simultaneous determination of nine marker substances in Ru-Yi-Jin-Huang-San. PMID:20082147

Lay, Horng-Liang; Chen, Chia-Chi; Huang, Shiow-Chyn; Cham, Thau-Ming; Wu, Tian-Shung; Lin, I-Hsin

2010-04-01

368

[Use of crude drugs in "PiWeiLun" in comparison to those used in "NeiWaiShangBianHuoLun".].  

PubMed

PiWeiLun (Hi-i-ron in Japanese) is a traditional Chinese medical formulary written in the 13th century that discusses endogenous disease. For curing endogenous diseases (Naisho) caused by functional deficiency of pi- and wei qi (Hi-I-Ki-Kyo), PiWeiLun was recommended for QingShuYiQi-Tang (Seisho-ekki-to), Ban-XiaBaiZhuTianMaTang (Hange-byakuju-tsu-tenma-to) and BuZhongYiQiTang (Ho-chu-ekki-to), which are described in NeiWaiShangBianHuoLun (Naigaisho-benwa-ku-ron).A characteristic of PiWeiLun is that it discusses combining the use of Phellodendri Cortex with sweet Qi-tonics (Hoki-yaku) as Ginseng and Atractylodis Macrocephalae Rhizome for improving false heat syndrome (Kyo-netsu) due to yin-deficiency (In-kyo). In PiWeiLun Cimicifugae Rhizome, Angelicae Radux and Atractylodes Rhizome are more frequently used than in NeiWaiShangBianHuoLun.The use of drugs presented in PiWeiLun may be useful to devise new crude drug formulations good for modern deficiency syndrome experienced by persons having post-operative complications. PMID:16217902

Fuwa, Takako; Kosoto, Hiroshi; Tani, Tadato

2005-01-01

369

Screening of 20 Commonly Used Iranian Traditional Medicinal Plants Against Urease  

PubMed Central

Infection with Helicobacter pyloriis the most common cause of stomach and duodenal ulcers. About more than 80 % of people are infected with H. pylori in developing countries. H. pylori uses urease enzyme product “ammonia” in order to neutralize and protect itself from the stomach acidic condition and urease enzyme activity has been shown to be essential to the colonization of H. pylori. Inhibitory activity of 20 traditional medicinal plants were examined and evaluated against Jack bean urease activity by Berthelot reaction to obtains natural sources of urease inhibitors. Each herb was extracted using 80% aqueous methanol, then tested its IC50 value was determined. Eight of the whole 20 studied plants crude extracts were found the most effective with IC50 values of less than 100 ?g/mL including Laurus nobilis, Zingiber officinale, Nigella sativa, Angelica archangelica, Acorus calamus, Allium sativum,Curcuma longa, and Citrus aurantium extracts, from which most potent urease inhibitory was observed for Zingiber officinale, Laurus nobilis, and Nigella sativa with IC50 values of 48.54, 48.69 and 59.10 ?g/mL, respectively.

Biglar, Mahmood; Sufi, Hessameddin; Bagherzadeh, Kowsar; Amanlou, Massoud; Mojab, Faraz

2014-01-01

370

Ligustilide prevents the apoptosis effects of tumour necrosis factor-alpha during C2C12 cell differentiation.  

PubMed

Ligustilide, the major component of Angelica sinensis, is also thought to be the most potent bioactive constituent of this plant. Ligustilide has been reported to markedly protect neural tissue against apoptosis. However, little is known regarding ligustilide's anti-apoptosis effect in muscle tissue. The aim of the study was to investigate the anti-apoptosis effects of ligustilide on TNF-?-induced C2C12 cells during differentiation. It was revealed that ligustilide at various concentrations significantly prevented the apoptosis of C2C12 cells incubated in TNF-? as assessed by apoptosis index and DNA fragmentation. Moreover, ligustilide-treated groups exhibited a significant increase in the bcl-2/bax ratio, pro-caspase 3 and pro-caspase 8 compared with the TNF-?-control group in a dose-dependent manner. Meanwhile, ligustilide-treated groups presented a significantly increased level of phosphorylated Akt and suppressed expression of the myogenin protein. Therefore, the findings derived suggested that ligustilide protected C2C12 cells from TNF-?-induced apoptosis during differentiation by reducing apoptosis and inducing cell proliferation. PMID:24560902

Shi, Ying; Wang, Dongtao; Lu, Lu; Yin, Yi; Wang, Ming; Li, Chengjie; Diao, Jianxin; Wang, Yanjing; Wei, Lianbo

2014-04-01

371

Ferulic acid regulates the AKT/GSK-3?/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model  

PubMed Central

Ferulic acid, a component of the plants Angelica sinensis (Oliv.) Diels and Ligusticum chuanxiong Hort, exerts a neuroprotective effect by regulating various signaling pathways. This study showed that ferulic acid treatment prevents the injury-induced increase of collapsin response mediator protein 2 (CRMP-2) in focal cerebral ischemia. Glycogen synthase kinase-3? (GSK-3?) regulates CRMP-2 function through phosphorylation of CRMP-2. Moreover, the pro-apoptotic activity of GSK-3? is inactivated by phosphorylation by Akt. This study investigated whether ferulic acid modulates the expression of CRMP-2 and its upstream targets, Akt and GSK-3?, in focal cerebral ischemia. Male rats were treated immediately with ferulic acid (100 mg/kg, i.v.) or vehicle after middle cerebral artery occlusion (MCAO), and then cerebral cortices were collected 24 hr after MCAO. MCAO resulted in decreased levels of phospho-Akt and phospho-GSK-3?, while ferulic acid treatment prevented the decrease in the levels of these proteins. Moreover, phospho-CRMP-2 and CRMP-2 levels increased during MCAO, whereas ferulic acid attenuated these injury-induced increases. These results demonstrate that ferulic acid regulates the Akt/GSK-3?/CRMP-2 signaling pathway in focal cerebral ischemic injury, thereby protecting against brain injury.

Gim, Sang-A; Sung, Jin-Hee; Shah, Fawad-Ali; Kim, Myeong-Ok

2013-01-01

372

Effects of Jin-Ying-Tang on Staphylococcus aureus-induced mastitis in rabbit.  

PubMed

The present study was performed to investigate the effects of Jin-Ying-Tang (JYT), a Chinese herbal formula containing Lonicera japonica, Herba taraxaci, Fructus trichosanthis, Fructus forsythia, Radix et rhizoma rhei, Astragalus membranaceus, Angelica sinensis, on rabbit mastitis induced by Staphylococcus aureus. Suckling rabbits were challenged with 1.5 × 10(7) colony forming unit (CFU) of S. aureus at the base of the third pair teats, and they were treated and pretreated with JYT to detect the formula effects. The results showed that JYT could reduce the occurrence of Staphylococcal mastitis in rabbit model. To further investigate the action mechanism of JYT, we examined the leukocyte counts and inflammatory mediator levels such as TNF-? and IL-6 in blood and infected tissue. From histological study and blood analysis, we found that JYT could suppress leukocyte infiltration in infected mammary gland tissue and significantly inhibit the total leukocyte counts and lymphocytes (LYM), monocytes (MON) and granulocytes (GRA) fractions of leukocyte counts in blood. Enzyme-linked immunosorbent assay (ELISA) results showed JYT significantly decreased the TNF-? and IL-6 concentrations in serum and mammary gland. The analysis of these data suggested that JYT effectively inhibited inflammatory responses to reduce the occurrence of mastitis in rabbit model. PMID:22303901

Wang, L U; He, Chang-Liang; He, Bing-Kun; Guo, Qing; Xiao, Chun-Gang; Yi, Qiong

2012-10-01

373

Antioxidant properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells in vitro.  

PubMed

A new chemical component, cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide, was isolated from Angelica sinensis and its structure elucidated from its NMR and MS spectra and confirmed by X-ray single crystal diffraction analysis. We also explored the antioxidative properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells (HUVECs) against injuries induced by hydrogen peroxide (H(2)O(2)) using an MTT assay and flow cytometry analysis. In addition, the activities of superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH), nitric oxide (NO) and reactive oxygen species (ROS) were determined. We found that cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide increased the viability of HUVECs injured by H(2)O(2) in a dose-dependent manner, reduced the apoptosis of HUVEC, and enhanced HUVEC proliferation. Our results demonstrated the remarkable in vitro antioxidative activities of this compound, indicating that it could be a potential antioxidant with protective effects against H(2)O(2)-induced HUVEC injuries. PMID:23282538

Li, Weidong; Wu, Yu; Liu, Xuedong; Yan, Cuiping; Liu, Dan; Pan, Yang; Yang, Guangming; Yin, Fangzhou; Weng, Zebin; Zhao, Ding; Chen, Zhipeng; Cai, Baochang

2013-01-01

374

Z-ligustilide activates the Nrf2/HO-1 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro.  

PubMed

Z-ligustilide (LIG), the main lipophilic component of Radix Angelica sinensis, has been shown to protect against brain ischemic damage in rodents by oral and intra-peritoneal treatments. The present study aimed to confirm the therapeutic effect of LIG administered intravenously on 2h middle cerebral artery occlusion (MCAO) and 22 h reperfusion injury in rats since oral administration has low bioavailability, slow absorption and distribution. Moreover, whether LIG activated the NF-E2-related factor 2/ heme oxygenase-1 (Nrf2/HO-1) pathway was also investigated in vivo and in vitro to further elucidate the precise protective mechanisms. In vivo, rats treated intravenously with LIG immediately after the surgery was finished had less neurological dysfunction and smaller infarct volume than that of the vehicle-treated rats. Additionally, LIG promoted Nrf2 nuclear translocation, and further remarkably increased Nrf2 and HO-1 protein expression. In vitro, LIG induced Nrf2 nuclear translocation and up-regulated HO-1 expression in a time-dependent and concentration-dependent manner. Furthermore, LIG treatment reduced cell death induced by OGD, however, the protective action was abolished while Nrf2/HO-1 expression was knockdown by RNA interference. These results noted that intravenous post-treatment with LIG exhibits noticeable neuroprotective properties against brain damage by ischemia-reperfusion and the ability of LIG to activate Nrf2/HO-1 pathway may be partly responsible for it. PMID:23688544

Peng, Bin; Zhao, Peng; Lu, Ya-Peng; Chen, Miao-Miao; Sun, Hua; Wu, Xiao-Mei; Zhu, Li

2013-07-01

375

Ferulic acid regulates the AKT/GSK-3?/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model.  

PubMed

Ferulic acid, a component of the plants Angelica sinensis (Oliv.) Diels and Ligusticum chuanxiong Hort, exerts a neuroprotective effect by regulating various signaling pathways. This study showed that ferulic acid treatment prevents the injury-induced increase of collapsin response mediator protein 2 (CRMP-2) in focal cerebral ischemia. Glycogen synthase kinase-3? (GSK-3?) regulates CRMP-2 function through phosphorylation of CRMP-2. Moreover, the pro-apoptotic activity of GSK-3? is inactivated by phosphorylation by Akt. This study investigated whether ferulic acid modulates the expression of CRMP-2 and its upstream targets, Akt and GSK-3?, in focal cerebral ischemia. Male rats were treated immediately with ferulic acid (100 mg/kg, i.v.) or vehicle after middle cerebral artery occlusion (MCAO), and then cerebral cortices were collected 24 hr after MCAO. MCAO resulted in decreased levels of phospho-Akt and phospho-GSK-3?, while ferulic acid treatment prevented the decrease in the levels of these proteins. Moreover, phospho-CRMP-2 and CRMP-2 levels increased during MCAO, whereas ferulic acid attenuated these injury-induced increases. These results demonstrate that ferulic acid regulates the Akt/GSK-3?/CRMP-2 signaling pathway in focal cerebral ischemic injury, thereby protecting against brain injury. PMID:23825478

Gim, Sang-A; Sung, Jin-Hee; Shah, Fawad-Ali; Kim, Myeong-Ok; Koh, Phil-Ok

2013-06-01

376

[Textual research on adulteration of Chinese materia medica in ancient China].  

PubMed

By investigating the mainstream works of herbal classics of successive ages, it is found that adulteration of Chinese materia medica appeared early in ancient China. The main methods of adulteration was producing fraud medicines in the Northern-Southern Dynasties, fake medicines began to appear in the Tang Dynasty, and status of adulteration of Chinese materia medica ran unchecked since the Ming and Qing Dynasty. By statistics, there were 76 kinds of adulteration varieties before the Republican period. The main varieties were precious drugs, animal drugs and artifacts. Commonly methods used in the process included forging and adulterating, dealing with 11 kinds and 68 kinds respectively. Adulteration probably lead to the result of imposing the changes of the used medicinal parts of Herba Pogostemonis; Radix Aconiti Lateralis prepared by adding salt, Radix Angelica Sinensis processed by wine, and Radix Astragalis seu Hedysaris processed with bee honey. However, the root cause of adulteration in Chinese materia medica was the dissociation of professional physician and pharmacist, resulting in the ignorance of medical practitioners became unable to recognize Chinese materia medica; and the immorality of medicinal merchants. Besides, rating the quality of materia medica based on its producing areas without differentiating the false from the genuine may also contribute to this result passively. PMID:24429032

Xie, Jin; Wang, De-Qun

2013-09-01

377

Traditional chinese medicine for senile dementia.  

PubMed

Traditional Chinese Medicine (TCM) has a 3000 years' history of human use. A literature survey addressing traditional evidence from human studies was done, with key result that top 10 TCM herb ingredients including Poria cocos, Radix polygalae, Radix glycyrrhizae, Radix angelica sinensis, and Radix rehmanniae were prioritized for highest potential benefit to dementia intervention, related to the highest frequency of use in 236 formulae collected from 29 ancient Pharmacopoeias, ancient formula books, or historical archives on ancient renowned TCM doctors, over the past 10 centuries. Based on the history of use, there was strong clinical support that Radix polygalae is memory improving. Pharmacological investigation also indicated that all the five ingredients mentioned above can elicit memory-improving effects in vivo and in vitro via multiple mechanisms of action, covering estrogen-like, cholinergic, antioxidant, anti-inflammatory, antiapoptotic, neurogenetic, and anti-A? activities. Furthermore, 11 active principles were identified, including sinapic acid, tenuifolin, isoliquiritigenin, liquiritigenin, glabridin, ferulic acid, Z-ligustilide, N-methyl-beta-carboline-3-carboxamide, coniferyl ferulate and 11-angeloylsenkyunolide F, and catalpol. It can be concluded that TCM has a potential for complementary and alternative role in treating senile dementia. The scientific evidence is being continuously mined to back up the traditional medical wisdom. PMID:21808655

Lin, Zhihong; Gu, Jie; Xiu, Jin; Mi, Tingyan; Dong, Jie; Tiwari, Jyoti Kumar

2012-01-01

378

Chinese herbs and their active ingredients for activating xue (blood) promote the proliferation and differentiation of neural stem cells and mesenchymal stem cells  

PubMed Central

Some Chinese herbs are anti-thrombolysis, and anti-inflammatory, improves brain RNA content, promotes brain protein synthesis, enhances dopamine function, regulates brain hormones, and improves microcirculation in central nervous system that might improve, repair and rehabilitation from the stroke and brain injury. Specific Chinese herbs and their components, such as Acanthopanax, Angelica, could maintain the survival of neural stem cells, and Rhodiola, Ganoderma spore Polygala, Tetramethylpyrazine, Gardenia, Astragaloside and Ginsenoside Rg1 promoted proliferation of neural stem cells, and Rhodiola, Astragaloside promoted differentiation of neural stem cell into neuron and glia in vivo. Astragalus, Safflower, Musk, Baicalin, Geniposide, Ginkgolide B, Cili polysaccharide, Salidroside, Astragaloside, Antler polypeptides, Ginsenoside Rg1, Panax notoginseng saponins promoted proliferation and differentiation of neural stem cells in vitro. Salvia, Astragalus, Ginsenoside Rg1, P. notoginseng saponins, Musk polypeptide, Muscone and Ginkgolide B promoted neural-directed differentiation of MSCs into nerve cells. These findings are encouraging further research into the Chinese herbs for developing drugs in treating patients of stroke and brain injury.

2014-01-01

379

Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells.  

PubMed

Acetylcholinesterase inhibitors are prominent alternative in current clinical treatment for AD patients. Therefore, there is a continued need to search for novel AChEIs with good clinical efficacy and less side effects. By using our in-house natural product database and AutoDock Vina as a tool in docking study, we have identified twelve phytochemicals (emodin, aloe-emodin, chrysophanol, and rhein in Rhei Radix Et Rhizoma; xanthotoxin, phellopterin, alloisoimperatorin, and imperatorin in Angelicae dahuricae Radix; shikonin, acetylshikonin, isovalerylshikonin, and ?,?-dimethylacrylshikonin in Arnebiae Radix) as candidates of AChEIs that were not previously reported in the literature. In addition to AChEI activity, a series of cell-based experiments were conducted for the investigation of their neuroprotective activities. We found that acetylshikonin and its derivatives prevented apoptotic cell death induced by hydrogen peroxide in human and rat neuronal SH-SY5Y and PC12 cells at 10??M. We showed that acetylshikonin exhibited the most potent antiapoptosis activity through the inhibition of the generation of reactive oxygen species as well as protection of the loss of mitochondria membrane potential. Furthermore, we identified for the first time that the upregulation of heme oxygenase 1 by acetylshikonin is a key step mediating its antiapoptotic activity from oxidative stress in SH-SY5Y cells. PMID:24302971

Wang, Yan; Pan, Wen-Liang; Liang, Wei-Cheng; Law, Wai-Kit; Tsz-Ming Ip, Denis; Ng, Tzi-Bun; Miu-Yee Waye, Mary; Chi-Cheong Wan, David

2013-01-01

380

Identification of marker compounds in herbal drugs on TLC with DART-MS.  

PubMed

This study was conducted to provide a more versatile and specific information on Thin Layer Chromatographic (TLC) analysis of medicinal plants. TLC plates developed with the extract of herbal medicines were analyzed with direct analysis in real time (DART) ion source. Three well known herbal drugs were extracted and developed on a silica-coated TLC plate with the conditions pre-established in Korean Pharmacopoeia IX. The developed plate was placed between the DART ion source and TOF-MS analyzer to get real time mass spectra from the bands on the TLC plate directly. The marker coumarin compounds, decursin and decursinol were successfully identified from the TLC plate developed with Angelicae gigantis radix, along with alkaloid compounds of rutaecarpine and evodiamine from Evodiae fructus, and lignan molecules of gomisin A, N, and schisandrin from Schisandrae fructus. This hyphenation system of TLC and DART-MS could provide unique and specific information on the major constituents of crude plant drug on TLC through uncovering high resolution mass number of each band on the TLC plate directly in real time. PMID:20945134

Kim, Hye Jin; Jee, Eun Hye; Ahn, Kwang Sung; Choi, Hyo Sook; Jang, Young Pyo

2010-09-01

381

Identification of metabolites of Buyang Huanwu decoction in rat urine using liquid chromatography-quadrupole time-of-flight mass spectrometry.  

PubMed

In the present study, rapid resolution liquid chromatography was coupled with quadrupole time-of-flight tandem mass spectrometry (RRLC-Q-TOF-MS) to identify the absorbed components and metabolites in rat urine after oral administration of Buyang Huanwu decoction (BYHWD). After oral administration of BYHWD, urine samples were collected and pretreated by solid phase extraction. The mass measurements were accurate within 5 ppm of error for all the protonated molecules, and subsequent fragment ions offered higher quality structural information for interpretation of the fragmentation pathways of various compounds. A total of 50 compounds were detected in rat urine samples within 20 min, including 12 parent compounds and 38 metabolites. Except for three prototype components (Hydroxysafflor yellow A, Paeoniflorin, and Amygdalin), the metabolites identified mainly came from Radix Astragali, Radix Angelicae Sinensis, and Rhizoma Chuanxiong. The results indicated that glucuronidation and sulfation were the major metabolic pathways of isoflavonoids, while glutathione conjugation, glucuronidation and sulfation were the main metabolic pathways of phthalides. No saponin-related metabolites were detected. The present study provided important structural information on the metabolism of BYHWD. Furthermore, the results of this work have demonstrated the feasibility of the RRLC/ESI-Q-TOF-MS approach for rapid and reliable characterization of metabolites from herbal medicines. PMID:22591804

Wen, Xiao-Dong; Liu, E-Hu; Yang, Jie; Li, Chang-Yin; Gao, Wen; Qi, Lian-Wen; Wang, Chong-Zhi; Yuan, Chun-Su; Li, Ping

2012-01-01

382

Identification of metabolites of Buyang Huanwu Decoction in rat urine using liquid chromatography-quadrupole time-of-flight mass spectrometry  

PubMed Central

In the present study, rapid resolution liquid chromatography was coupled with quadrupole time-of-flight tandem mass spectrometry (RRLC-Q-TOF-MS) to identify the absorbed components and metabolites in rat urine after oral administration of Buyang Huanwu decoction (BYHWD). After oral administration of BYHWD, urine samples were collected and pretreated by solid phase extraction. The mass measurements were accurate within 5 ppm of error for all the protonated molecules, and subsequent fragment ions offered higher quality structural information for interpretation of the fragmentation pathways of various compounds. A total of 50 compounds were detected in rat urine samples within 20 min, including 12 parent compounds and 38 metabolites. Except for three prototype components (Hydroxysafflor yellow A, Paeoniflorin, and Amygdalin), the metabolites identified mainly came from Radix Astragali, Radix Angelicae Sinensis, and Rhizoma Chuanxiong. The results indicated that glucuronidation and sulfation were the major metabolic pathways of isoflavonoids, while glutathione conjugation, glucuronidation and sulfation were the main metabolic pathways of phthalides. No saponin-related metabolites were detected. The present study provided important structural information on the metabolism of BYHWD. Furthermore, the results of this work have demonstrated the feasibility of the RRLC/ESI-QTOF-MS approach for rapid and reliable characterization of metabolites from herbal medicines.

Wen, Xiao-Dong; Liu, E-Hu; Yang, Jie; Li, Chang-Yin; Gao, Wen; Qi, Lian-Wen; Wang, Chong-Zhi; Yuan, Chun-Su; Li, Ping

2012-01-01

383

Vegetational response to human colonisation of the coastal and volcanic environments of Ketilsstaðir, southern Iceland  

NASA Astrophysics Data System (ADS)

Tephra-dated, high-resolution pollen profiles from Ketilsstaðir, southern Iceland, indicate a largely unwooded pre-settlement environment, a probable consequence of the exposed coastal location. The degree of change associated with the Norse landnám is more limited than in many Icelandic pollen diagrams. There are three main periods of change in the post-settlement vegetational development of the area. Firstly, Norse settlement affected the hydrology of the bog, resulting in the near-disappearance of Sphagnum and agricultural activity led to a reduction of some species (e.g. Angelica spp. and, Salix). Secondly, the establishment of probable permanent settlement in the mid-11th century AD initiated expansion of such apophytic taxa as Plantago spp. Lactuceae, Ranunculus spp. and Pteridophytes. Thirdly, the ? 10 cm thick Katla tephra, deposited in AD 1357, enhanced drainage of the bog surface, favouring dryland taxa (e.g. Poaceae, Galium and Lactuceae). The tephra deposit and the associated drainage probably caused or contributed to the local extinction of the wetland beetle Hydraena britteni. The study has enabled a series of natural and humanly-related issues to be addressed including tephra-vegetation relationships, the anthropogenic reduction in plant diversity, and comparisons between historical and environmental settlement records.

Erlendsson, Egill; Edwards, Kevin J.; Buckland, Paul C.

2009-09-01

384

Position of the Upper Devonian Frasnian--Famennian boundary in the central Appalachians  

SciTech Connect

Biostratigraphic analysis of eight Upper Devonian sections in VA and WV reveals that the section at Huttonsville, Randolph County, WV, is a key locality for determining the Frasnian-Famennian boundary. The Foreknobs Formation at Huttonsville indicates a higher stratigraphic placement of the Frasnian-Famennian boundary than has been generally assumed. Conodonts are not known within that section, so placement of the boundary uses the last occurrence of tentaculitids and the last and first occurrences of several species of brachiopods. It is believed that the Frasnian-Famennian boundary can be identified independently of the atrypoid brachiopods. Stratigraphic ranges of the cricoconarid Tentaculites discusses and the brachiopod Tropidoleptus carinatus, both considered Frasnian marker fossils, indicate that the Frasnian extends well into the Red Lick Member of the Foreknobs Formation at Huttonsville, with T. carinatus occurring up to 70% of the stratigraphic thickness of the Red Lick. The Famennian marker fossils A. angelica and C. sulcifer are both found at Huttonsville above the last recorded occurrence of all the Frasnian marker fossils. To the northeast of Huttonsville the Frasnian-Famennian series boundary has been placed by other workers within or at the top of the Pound Member of the Foreknobs Formation. This discrepancy implies that either the Pound Member is diachronous or that to the northeast paleoecologic factors caused local disappearances of critical fossils before their extinction at Huttonsville.

Rossbach, T.J. (Univ. of North Carolina, Chapel Hill, NC (United States). Dept. of Geology)

1992-01-01

385

Genetic diversity in populations of plants with different breeding and dispersal strategies in a free-flowing boreal river system.  

PubMed

We have studied the genetic diversity of three plant species: Angelica archangelica (Apiacieae), Bistorta vivipara (Polygonaceae) and Viscaria alpina (Caryophyllaceae) along the free-flowing Vindel River in northern Sweden. The plants differ in reproductive strategy. A archangelica and V. alpina are insect pollinated outbreeders while B. vivipara reproduces with apomixis through bulbils. The seeds of A. archangelica may float for over a year, while the propagules (seeds and bulbils, respectively) of V. alpina and B. vivipara float for less than two days. Genetic diversity was assessed using starch gel electrophoresis. The clonal diversity of B. vivipara measured by Simpson's index (D) ranged between 0.78 and 0.99. Only a few clones were shared between localities. The average percentages polymorphic loci and mean He based on polymorphic loci for V. alpina over all localities were 23.1 and 0.15, respectively. Wright's F-statistics showed a significant overall deficit of heterozygotes. The diversity of A. archangelica was found to increase downstream. Genetic diversity of each species is sufficiently high to be used in studies on hydrochory. Dispersal appears to be related to the floating ability of progagules. PMID:12035618

Lundqvist, E; Andersson, E

2001-01-01

386

Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination.  

PubMed

Extracts from the plants Iberis amara, Melissa officinalis, Matricaria recutita, Carum carvi, Mentha x piperita, Glycyrrhiza glabra, Angelica archangelica, Silybum marianum and Chelidonium majus, singly and combined in the form of a commercial preparation, STW 5 (Iberogast) and a modified formulation, STW 5-II, lacking the last 3 constituents, were tested for their potential anti-ulcerogenic activity against indometacin induced gastric ulcers of the rat as well as for their antisecretory and cytoprotective activities. All extracts produced a dose dependent anti-ulcerogenic activity associated with a reduced acid output and an increased mucin secretion, an increase in prostaglandin E2 release and a decrease in leukotrienes. The effect on pepsin content was rather variable and did not seem to bear a relationship with the anti-ulcerogenic activity. The most beneficial effects were observed with the combined formulations STW 5 and STW 5-II in a dose of 10 ml/kg b.w., comparable with cimetidine in a dose of 100 mg/kg b.w. The anti-ulcerogenic activity of the extracts was also confirmed histologically. The cytoprotective effect of the extracts could be partly due to their flavonoid content and to their free radical scavenging properties. PMID:11505785

Khayyal, M T; el-Ghazaly, M A; Kenawy, S A; Seif-el-Nasr, M; Mahran, L G; Kafafi, Y A; Okpanyi, S N

2001-01-01

387

Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in RAW264.7 cells  

PubMed Central

The objective of the present study was to analyze the effect of a mixture of medicinal plants [Angelica gigas Nakai, Panax ginseng and Rhus verniciflua Stokes (APR)] on lipopolysaccharide (LPS)-induced inflammatory responses in the murine macrophage cell line RAW264.7. Cells were treated with APR and LPS at various concentrations and indicated times. WST assay, trypan blue assay and quantification of activated cells demonstrated that APR suppressed cell proliferation in a dose-dependent manner. APR induced G1 cell cycle arrest and inhibited the LPS-induced phosphorylation of protein kinase B (AKT), extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (p38) and necrosis factor ?B (NF-?B). APR also suppressed nitric oxide synthase isoform (iNOS) and prostaglandin endoperoxide synthase 2 (Cox-2) messenger ribonucleic acid (mRNA) expression induced by LPS. Furthermore, APR decreased LPS-induced intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, as well as induced PARP and caspase-3 cleavage, suggesting that APR causes apoptosis. In conclusion, the present study indicated that APR may be advantageous in treating inflammatory disease.

CHOI, HAN-SEOK; SEO, HYE SOOK; KIM, SOON RE; CHOI, YOUN KYUNG; SHIN, YONG-CHEOL; KO, SEONG-GYU

2014-01-01

388

Antibacterial and anti-inflammatory effects of Jeju medicinal plants against acne-inducing bacteria.  

PubMed

Propionibacterium acnes and Staphylococcus epidermidis are pus-forming bacteria that trigger inflammation in acne. The present study was conducted to evaluate the antimicrobial activities of Jeju medicinal plants against these etiologic agents of acne vulgaris. Ethanol extracts of Jeju plants were tested for antimicrobial activities by disc diffusion and broth dilution methods. The results from the disc diffusion assays revealed that four medicinal plants, Mollugo pentaphylla, Angelica anomala, Matteuccia orientalis, and Orixa japonica inhibited the growth of both pathogens. Among these, A. anomala had strong inhibitory effects. Its MIC values were 15.6 microg/ml and 125 microg/ml against P. acnes and S. epidermidis, respectively. The cytotoxic effects of the four extracts were determined by colorimetric MTT assays using two animal cell lines: human dermal fibroblasts and HaCaT cells. Although the M. orientalis root extract had moderate cytotoxicity in HaCaT cells at 200 microg/ml, most extracts exhibited low cytotoxicity at 200 microg/ml in both cell lines. In addition, the extracts reduced the P. acnes-induced secretion of interleukin-8 and tumor necrosis factor-alpha (TNF-alpha) in THP-1 cells, an indication of their anti-inflammatory effects. Based on these results, we suggest that M. pentaphylla, A. anomala, M. orientalis, and O. japonica are attractive acne-mitigating candidates for topical application. PMID:18497484

Kim, Sang-Suk; Kim, Ji-Young; Lee, Nam Ho; Hyun, Chang-Gu

2008-04-01

389

Study on structural and spectral properties of isobavachalcone and 4-hydroxyderricin by computational method  

NASA Astrophysics Data System (ADS)

Isobavachalcone and 4-hydroxyderricin, two major chalcone constituents isolated from the roots of Angelica keiskei KOIDZUMI, exhibit numerous biological activities. Quantum chemical methods have been employed to investigate their structural and spectral properties. The ground state structures were optimized using density functional B3LYP method with 6-311G (d, p) basis set in both gas and solvent phases. Based on the optimized geometries, the harmonic vibrational frequency, the 1H and 13C nuclear magnetic resonance (NMR) chemical shift using the GIAO method were calculated at the same level of theory, with the aim of verifying the experimental values. Results reveal that B3LYP has been a good method to study their vibrational spectroscopic and NMR spectral properties of the two chalcones. The electronic absorption spectra were calculated using the time-dependent density functional theory (TDDFT) method. The solvent polarity effects were considered and calculated using the polarizable continuum model (PCM). Results also show that substitutions of different electron donating groups can alter the absorption properties and shift the spectra to a higher wavelength region.

Rong, Yuzhi; Wu, Jinhong; Liu, Xing; Zhao, Bo; Wang, Zhengwu

390

Application of the equivalency factor concept to the phototoxicity and -genotoxicity of furocoumarin mixtures.  

PubMed

Furocoumarins (FCs) are natural constituents widely occurring in plants used as food or in phytomedicines, cosmetics, etc. Some FCs exert dermal photo-toxicity and -genotoxicity when combined with UVA irradiation. For a few congeners, skin tumor formation has been described in humans and laboratory animals. Since almost no information is available on the photo-toxic properties of several congeners, we analyzed the photo-cytotoxic, photo-mutagenic, and photo-clastogenic properties in V79 cells for thirteen naturally occurring FCs, and for the coumarin limettin. Furthermore, nine FC mixtures including one mixture based on the FC pattern of an Angelica archangelica extract were tested in the same assays. We found that the concept of relative potency factors for photo-cytotoxic, -mutagenic, and -clastogenicpotencies of FCs, setting the value for 5-methoxypsoralen at 1.00, was applicable to all congeners tested. The concept was used successfully to describe the photo-toxic properties of binary mixtures of 5- and 8-methoxypsoralen. Furthermore, the photo-genotoxic (photo-mutagenic and -clastogenic) properties of complex FC mixtures comprising up to nine different congeners could be predicted. These data suggest that FCs can differ widely in their photo-toxic and photo-genotoxic properties but show relatively strict additivity with respect to their on target-effects when occurring as complex mixtures. PMID:24680798

Raquet, Nicole; Schrenk, Dieter

2014-06-01

391

Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1 (TRPV1) channel.  

PubMed

Furanocoumarin imperatorin is the major active component of Angelica dahurica root extracts, widely used in traditional medicine to treat headache, toothache, and orbital eye pain. In this study, we investigated the mechanisms that may underlie the pain-relieving effects of the compound. We found that imperatorin significantly inhibited formalin- and capsaicin-induced nocifensive responses but did not alter baseline thermal withdrawal thresholds in the rat. We established that imperatorin is a weak agonist of TRPV1, a channel implicated in detecting several noxious stimuli, exhibiting a 50% effective concentration (EC50) of 12.6 ± 3.2 ?M. A specific TRPV1 antagonist, JNJ-17203212 (0.5 ?M), potently inhibited imperatorin-induced TRPV1 activation. Site-directed mutagenesis studies revealed that imperatorin most likely acted via a site adjacent to or overlapping with the TRPV1 capsaicin-binding site. TRPV1 recovery from desensitization was delayed in the presence of imperatorin. Conversely, imperatorin sensitized TRPV1 to acid activation but did not affect the current amplitude and/or the activation-inactivation properties of Na(v)1.7, a channel important for transmission of nociceptive information. Thus, our data indicate that furanocoumarins represent a novel group of TRPV1 modulators that may become important lead compounds in the drug discovery process aimed at developing new treatments for pain management. PMID:24569998

Chen, Xingjuan; Sun, Weiyang; Gianaris, Nicholas G; Riley, Ashley M; Cummins, Theodore R; Fehrenbacher, Jill C; Obukhov, Alexander G

2014-04-01

392

Hydrolysis of Glycosidic Flavonoids during the Preparation of Danggui Buxue Tang: An Outcome of Moderate Boiling of Chinese Herbal Mixture.  

PubMed

Chemical change during boiling of herbal mixture is a puzzle. By using Danggui Buxue Tang (DBT), a herbal decoction that contains Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), we developed a model in analyzing the hydrolysis of flavonoid glycosides during the boiling of herbal mixture in water. A proper preparation of DBT is of great benefit to the complete extraction of bioactive ingredients. Boiling of DBT in water increased the solubility of AR-derived astragaloside IV, calycosin, formononetin, calycosin-7-O- ? -D-glucoside, and ononin in a time- and temperature-dependent manner: the amounts of these chemicals reached a peak at 2?h. The glycosidic resides of AR, calycosin-7-O- ? -D-glucoside, and ononin could be hydrolyzed during the moderate boiling process to form calycosin and formononetin, respectively. The hydrolysis efficiency was strongly affected by pH, temperature, and amount of herbs. Interestingly, the preheated herbs were not able to show this hydrolytic activity. The current results supported the rationality of ancient preparation of DBT in boiling water by moderate heat. PMID:24744813

Zhang, Wendy Li; Chen, Jian-Ping; Lam, Kelly Yin-Ching; Zhan, Janis Ya-Xian; Yao, Ping; Dong, Tina Ting-Xia; Tsim, Karl Wah-Keung

2014-01-01

393

Chinese herbs and their active ingredients for activating?xue?(blood)?promote the proliferation and differentiation of neural stem cells and mesenchymal stem cells.  

PubMed

Some Chinese herbs are anti-thrombolysis, and anti-inflammatory, improves brain RNA content, promotes brain protein synthesis, enhances dopamine function, regulates brain hormones, and improves microcirculation in central nervous system that might improve, repair and rehabilitation from the stroke and brain injury. Specific Chinese herbs and their components, such as Acanthopanax, Angelica, could maintain the survival of neural stem cells, and Rhodiola, Ganoderma spore Polygala, Tetramethylpyrazine, Gardenia, Astragaloside and Ginsenoside Rg1 promoted proliferation of neural stem cells, and Rhodiola, Astragaloside promoted differentiation of neural stem cell into neuron and glia in vivo. Astragalus, Safflower, Musk, Baicalin, Geniposide, Ginkgolide B, Cili polysaccharide, Salidroside, Astragaloside, Antler polypeptides, Ginsenoside Rg1, Panax notoginseng saponins promoted proliferation and differentiation of neural stem cells in vitro. Salvia, Astragalus, Ginsenoside Rg1, P. notoginseng saponins, Musk polypeptide, Muscone and Ginkgolide B promoted neural-directed differentiation of MSCs into nerve cells. These findings are encouraging further research into the Chinese herbs for developing drugs in treating patients of stroke and brain injury. PMID:24716802

Si, Yin-Chu; Li, Qiang; Xie, Chun-E; Niu, Xin; Xia, Xiao-Hui; Yu, Chang-Yuan

2014-01-01

394

Estonian folk traditional experiences on natural anticancer remedies: From past to the future.  

PubMed

Abstract Context: Despite diagnostic and therapeutic advancements, the burden of cancer is still increasing worldwide. Toxicity of current chemotherapeutics to normal cells and their resistance to tumor cells highlights the urgent need for new drugs with minimal adverse side effects. The use of natural anticancer agents has entered into the area of cancer research and increased efforts are being made to isolate bioactive products from medicinal plants. Objective: To lead the search for plants with potential cytotoxic activity, ethnopharmacological knowledge can give a great contribution. Therefore, the attention of this review is devoted to the natural remedies traditionally used for the cancer treatment by Estonian people over a period of almost 150 years. Methods: Two massive databases, the first one stored in the Estonian Folklore Archives and the second one in the electronic database HERBA ( http://herba.folklore.ee/ ), containing altogether more than 30?000 ethnomedicinal texts were systematically reviewed to compile data about the Estonian folk traditional experiences on natural anticancer remedies. Results and conclusion: As a result, 44 different plants with potential anticancer properties were elicited, 5 of which [Angelica sylvestris L. (Apiaceae), Anthemis tinctoria L. (Asteraceae), Pinus sylvestris L. (Pinaceae), Sorbus aucuparia L. (Rosaceae), and Prunus padus L. (Rosaceae)] have not been previously described with respect to their tumoricidal activities in the scientific literature, suggesting thus the potential herbal materials for further investigations of natural anticancer compounds. PMID:24920231

Sak, Katrin; Jürisoo, Kadi; Raal, Ain

2014-07-01

395

Aphid parasitoid complex in potato in the context of IPM in Belgium.  

PubMed

Parasitic hymenoptera of potato aphids were collected and identified in 2000 and 2001 in 11 potato fields in Belgium. Nine Aphidiidae species, parasiting seven aphid species, were found: Aphidius ervi, Aphidius matricariae, Aphidius picipes, Binodoxys angelicae, Diaeretiella rapae, Praon abjectum, Praon gallicium, Praon volucre and Toxares deltiger. A. ervi and A. picipes were the dominant and sub-dominant species, with 54% and 28% of the primary parasite collected, respectively. Both species and T. deltiger were found on Aphis nasturti, Aulacorthum solani, Macrosiphum euphorbiae and Myzus persicae, the four most important aphid potato in Belgium. Parasitism rate of A. nasturtii and, to a lesser extent, M. euphorbiae was low compared to A. solani and M. persicae. Parasitism of A. solani was particularly high, with 63.5% in 2000 and 89.2% in 2001, and this species was the preferred host of several Aphidiid species. The abundance of alternative hosts as other crops pest aphids or non-pest aphids on wild plants in agroecosystems could explains the efficacy of these species. The biological control of A. nasturtii and M. euphorbiae by parasitic hymenoptera was poorer, and several studies need to be undertaken to find suitable parasitic hymenoptera species effective on these aphids and agro-environmental measures able to promote them. PMID:16628888

Jansen, J-P

2005-01-01

396

Screening of Chinese herbal drug extracts for inhibitory activity on nitric oxide production and identification of an active compound of Zanthoxylum bungeanum.  

PubMed

Sixty-eight water and methanol extracts from 34 Chinese herbal drugs, most of which are used for inflammatory diseases, were screened for their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated J774.1 macrophages and in LPS/interferon (IFN)-gamma-stimulated mouse peritoneal exudate macrophages. Among the extracts, methanol extracts of Myristica fragrans, Plantago asiatica, Rubia cordifolia, and Zanthoxylum bungeanum showed significant inhibition in J774.1 macrophages, while in mouse peritoneal exudate macrophages, water extracts of Ru. cordifolia and Scutellaria baicalensis and methanol extracts of Angelica megaphylla, My. fragrans, and Z. bungeanum inhibited the NO production. Among them, inhibition of water extract of Sc. baicalensis was found to be mainly due to direct scavenging of NO radicals, through an examination of its scavenging activity on PAPA NONOate-generated NO radicals, while water extract of Ru. cordifolia and methanol extracts of An. megaphylla, My. fragrans, P. asiatica, and Z. bungeanum showed inhibition on iNOS mRNA expression. At last, an inhibitory compound on iNOS mRNA expression was isolated from a methanol extract of Z. bungeanum and identified as 4-O-beta-D-glucopyranosyldihydroferulic acid by NMR spectral analyses and chemical synthesis. PMID:11535366

Tezuka, Y; Irikawa, S; Kaneko, T; Banskota, A H; Nagaoka, T; Xiong, Q; Hase, K; Kadota, S

2001-10-01

397

Antioxidant and antimicrobial capacity of Chinese medicinal herb extracts in raw sheep meat.  

PubMed

Antioxidant and antimicrobial properties of 10 Chinese medicinal herb extracts were evaluated by dipping raw sheep meat in extracts, packaging the samples in polyethylene, and refrigerating them at 4 degrees C. The optimum concentrations of Codonopsis pilosula, Platycodon grandiflorum, Artemisia capillaris, Cinnamomum cassia, Rheum palmatum, Ziziphus jujuba, Gardenia jasminoides, Santalum album, Angelica sinensis, and Bletilla striata were 0.10, 0.10, 0.25, 0.10, 0.25, 0.25, 0.25, 0.10, 0.25, and 0.25%, respectively. Analysis revealed that test ingredients were more effective in reducing lipid oxidation and microbial counts in raw sheep meat. Correlation analysis revealed a significant negative linear relationship between the inhibition of hydroxyl and lipid oxidation, and inhibition of hydroxyl was the main factor affecting lipid oxidation. A. capillaris (0.25%), C. pilosula (0.10%), and P. grandiflorum (0.10%) were identified as the most effective antioxidants. S. album (0.10%), A. capillaris (0.10%), and C. cassia (0.10%) were the most effective antimicrobials. A. capillaris (0.25%), C. pilosula (0.10%), and P. grandiflorum (0.10%) increased meat redness significantly (P < 0.05) when compared with the control samples on days 0, 3, 5, 7, 9, 10, and 11. The pH values of sheep meat treated with C. pilosula (0.10%) and A. capillaris (0.10%) were lower than those of meat treated with other extracts. PMID:17612075

Luo, Hongxia; Lin, Shaohua; Ren, Fazheng; Wu, Liping; Chen, Lishui; Sun, Yan

2007-06-01

398

Imperatorin induces Mcl-1 degradation to cooperatively trigger Bax translocation and Bak activation to suppress drug-resistant human hepatoma.  

PubMed

Imperatorin is a small molecule nature compound isolated from the root of Angelica dahurica, and has been shown to exhibit multiple bioeffector functions, including anti-cancer activity. However, the molecular mechanism underlying imperatorin in suppression of tumor growth is unknown. In this study, we aimed at elucidating the molecular mechanisms underlying imperatorin function and determining the efficacy of imperatorin in suppression of drug-resistant human liver cancer. We observed that imperatorin suppresses tumor cell growth through inducing apoptosis, and imperatorin is more effective in induction of multidrug-resistant human liver cancer cells in vitro. We further determined that imperatorin induces apoptosis through both extrinsic and intrinsic apoptosis pathway. At the molecular level, we identified Mcl-1 as the molecular target of imperatorin and determined that imperatorin induces proteosome-dependent Mcl-1 degradation to release Bak and Bax to trigger apoptosis in liver cancer cells. Consistent with its in vitro apoptosis induction activity, imperatorin exhibited potent activity against multidrug-resistant liver cancer xenograft growth in vivo. Taken together, we determined that imperatorin is a Mcl-1 degradation inducer that can effectively suppress multidrug-resistant human liver cancer growth in vivo, and thus holds great promise for development as an effective small molecule anti-cancer agent in human liver cancer therapy to overcome drug resistance. PMID:24680709

Li, Xia; Zeng, Xueli; Sun, Jianguo; Li, Hua; Wu, Ping; Fung, Kwok-Pui; Liu, Feiyan

2014-06-28

399

Melanogenesis stimulation in murine b16 melanoma cells by umberiferae plant extracts and their coumarin constituents.  

PubMed

Melanogenesis stimulation activities of seven ethanolic extracts obtained from Umbelliferae plants used as Chinese crude drugs, namely the roots of Angelica dahurica BENTH. et HOOK., A. biserrata SHEN et YUAN, Notopterygium incisum TING, Heracleum lanatum MICHX., and H. candicans WALL., and the fruits of Cinidium monnieri (L.) CUSSON and C. formosanum YABE, were examined by using cultured murine B16 melanoma cells. Among them, the extract (5, 25 microg/ml) of H. lanatum showed a potent stimulatory effect on melanogenesis with significant enhancement of cell proliferation in a dose-dependent manner. The melanogenesis stimulatory effects of sixteen coumarins (1-16) isolated from the seven Umbelliferae crude drugs were also examined. Among them, linear-furocoumarins [psoralen (1), xanthotoxin (2), bergapten (3), and isopimpinellin (4)] and angular-furocoumarin [sphondin (13)] exhibited potent melanogenesis stimulation activity. From the view point of structure-activity relationships, it may be assumed that a linear-furocoumarin ring having a hydrogen and/or methoxyl group at 5 and 8 positions such as 1, 2, 3 and 4 was preferable for the melanogenesis stimulation activity. The introduction of a prenyl group into the furocoumarin ring was disadvantageous. Coumarin derivatives having a simple coumarin ring were inactive. PMID:15997104

Matsuda, Hideaki; Hirata, Noriko; Kawaguchi, Yoshiko; Yamazaki, Miho; Naruto, Shunsuke; Shibano, Makio; Taniguchi, Masahiko; Baba, Kimiye; Kubo, Michinori

2005-07-01

400

Pharmacokinetic interactions between herbal remedies and medicinal drugs.  

PubMed

1. The use of herbal products to treat a wide range of conditions is rising rapidly, leading to increased intake of phytochemicals. Recent studies revealed potentially fatal interactions between herbal remedies and traditional drugs. 2. In transplant patients, self-medication with St John's wort (Hypericum perforatum) has led to a drop in plasma levels of the immunosuppressant drug cyclosporine, causing tissue rejection. 3. Intake of St John's wort increases the expression of intestinal P-glycoprotein and the expression of CYP3A4 in the liver and intestine. The combined up-regulation in intestinal P-glycoprotein and hepatic and intestinal CYP3A4 impairs the absorption and stimulates the metabolism of cyclosporine, leading to subtherapeutic plasma levels. The St John's wort component, hyperforin, contributes to the induction of CYP3A4. 4. St John's wort also enhances the metabolism of other CYP3A4 substrates including the protease inhibitors indinavir and nevirapine, oral contraceptives, and tricyclic antidepressants such as amitriptyline. 5. Other herbal remedies with the potential to modulate cytochrome P450 activity and thus participate in interactions with conventional drugs include Milk thistle, Angelica dahurica, ginseng, garlic preparations, Danshen and liquorice. 6. Herbal products are currently not subject to the rigorous testing indispensable for conventional drugs. However, if potential drug interactions are to be predicted, it is essential that the ability of herbal products to interfere with drug-metabolizing enzyme systems is fully established. PMID:12160480

Ioannides, C

2002-06-01

401

Inhibition of Trypanosoma cruzi growth by medical plant extracts.  

PubMed

This study describes the screening of extracts obtained from 18 plants and two fungi used in the Chinese and Mediterranean traditional medicines on epimastigote forms of Trypanosoma cruzi. The extracts were tested against epimastigote of T. cruzi Bra C15C2 clone in vitro at 27 degrees C and at a concentration of 250 microg/ml in axenic culture. Angelica dahurica, A. pubescens, A. sinensis, Astragalus membranaceus, Coptis chinensis, Haplophyllum hispanicum, Phellodendron amurense, Poria cocos, Ranunculus sceleratus and Scutellaria baicalensis showed significant effects against the parasite with a percentage of growth inhibition between 20 and 100%. C. chinensis and R. sceleratus showed the greatest activity with IC(50) values of 1.7 microg/ml for C. chinensis and 10.7 microg/ml for R. sceleratus. These activities are greater than that of allopurinol. C. chinesis and R. sceleratus extracts did not show cytotoxic effects on rat polimorphonuclear cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and lactic dehydrogenase assays. These results allowed us to suggest that R. sceleratus and C. chinensis could be a source of new compounds clinically active against T. cruzi. PMID:12490214

Schinella, G R; Tournier, H A; Prieto, J M; Ríos, J L; Buschiazzo, H; Zaidenberg, A

2002-12-01

402

Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells.  

PubMed

The anoikis-sensitization activity of imperatorin, an active furanocoumarin component of Angelica dahurica root, is reported herein for the first time. The present study demonstrated that the imperatorin treatment at sub-toxic concentrations enhanced human lung cancer H23 cell apoptosis after detachment. A Western blot analysis showed that imperatorin significantly enhanced the p53 protein level, which subsequently down-regulated Mcl-1 protein and up-regulated Bax, while it had a minimal effect on Bcl-2 expression. In addition, an anchorage-independent growth assay was performed to support the anti-metastasis potential of imperatorin. Consistent with anoikis assay, imperatorin exhibited a strong inhibitory effect on the anchorage-independent growth of the cells. Further, this study demonstrated that imperatorin sensitizes anoikis in other lung cancer cells, namely, H292 and A549. Because anoikis was shown to be a critical hindrance in preventing cancer cell metastasis, the knowledge regarding such an activity and an underlying mechanism may lead to the development of this compound for a cancer therapy. PMID:23108812

Choochuay, Kanuengnit; Chunhacha, Preedakorn; Pongrakhananon, Varisa; Luechapudiporn, Rataya; Chanvorachote, Pithi

2013-07-01

403

Effects of oxypeucedanin on hKv1.5 and action potential duration.  

PubMed

A furocoumarin derivative, oxypeucedanin, was purified from Angelica dahurica, and its effects on the human Kv1.5 (hKv1.5) channel and on the cardiac action potential duration (APD), were examined using the patch-clamp technique and the conventional microelectrode technique. Oxypeucedanin inhibited the hKv1.5 current in a concentration-dependent manner, with an IC(50) value of 76 nM, while it had no effect on human eag-related gene (HERG) current. Oxypeucedanin induced an initial fast decline of hKv1.5 current during depolarizations. The inhibition of hKv1.5 channel by oxypeucedanin was voltage-dependent, especially at depolarizing pulses between -40 and 0 mV which corresponds to the voltage range of the channel's opening. Oxypeucedanin also slowed the deactivation time course, resulting in a tail crossover phenomenon. Additionally, oxypeucedanin prolonged the APD of rat atrial and ventricular muscles in a dose-dependent manner. These results suggest that oxypeucedanin is a kind of open-channel blocker of the hKv1.5 channel and it prolongs the APD; therefore, it is an excellent candidate as an antiarrhythmic drug for atrial fibrillation. PMID:15802805

Eun, Jae Soon; Park, Jung Ah; Choi, Bok Hee; Cho, Sun Kyung; Kim, Dae Keun; Kwak, Yong Geun

2005-04-01

404

Simultaneous determination of byak-angelicin and oxypeucedanin hydrate in rat plasma by column-switching high-performance liquid chromatography with ultraviolet detection.  

PubMed

A simple and sensitive column-switching HPLC method was developed for the simultaneous determination of two furocoumarin compounds, byak-angelicin and oxypeucedanin hydrate, which are the main components of hot water extract of Angelica dahurica root (AE), in rat plasma. Plasma sample was simply deproteinated with perchloric acid. After centrifugation, the supernatant was injected into a column-switching HPLC system consisting of a clean-up column (Symmetry Shield RP 8, 20x3.9 mm I.D.) and analytical column (Symmetry C18, 75x4.6 mm I.D.) which were connected with a six-port switching valve. The flow-rate of the mobile phase (acetonitrile-water, 20:80) was maintained at 1 ml/min. Detection was carried out at wavelength 260 nm with a UV detector. The column temperature was maintained at 40 degrees C. The calibration curves of byak-angelicin and oxypeucedanin hydrate were linear over the ranges 19.6 to 980 ng/ml (r2>0.997). The accuracy of these analytes was less than 4.4%. The intra- and inter-day relative standard deviations of byak-angelicin and oxypeucedanin hydrate were within 12.0% and 12.7%, respectively. The present method was applied for the analysis of plasma concentration from rats after administration of AE. PMID:11334345

Ishihara, K; Fukutake, M; Asano, T; Mizuhara, Y; Wakui, Y; Yanagisawa, T; Kamei, H; Ohmori, S; Kitada, M

2001-04-01

405

A bioassay using Artemia salina for detecting phototoxicity of plant coumarins.  

PubMed

Artemia salina (brine shrimp) has been successfully used for toxicity testing, and a screening test for phototoxicity has been developed based on this method. The ability of the method to test the phototoxic potential of seven known compounds was investigated. Athamantin (an angular furanocoumarin) and umbelliferone (a simple coumarin) showed no phototoxicity, while linear furanocoumarins exhibited phototoxic activity in the following order: psoralen > bergapten > peucedanin > xanthotoxin. The applicability of this method was also tested in screening the phototoxicity of plant material. Six plants from Apiaceae [Aegopodium podagraria L., Anethum graveolens L., Angelica archangelica L., Levisticum officinalis Koch, Petroselinum crispum (P. Mill) A. W. Hill., and Peucedanum palustre (L.) Moench] and one from Rutaceae (Ruta graveolens L.) were selected, all of them known to contain furanocoumarins. Extracts from leaves collected at different times during the growth period were used in the screening. Our results were in accordance with the furanocoumarin content of these plants and with the results of other phototoxicity tests. The Artemia salina method proved to be rapid, simple and inexpensive, and is therefore ideal in the initial biological screening of large numbers of samples for simultaneous detection of both toxicity and phototoxicity. PMID:10630112

Ojala, T; Vuorela, P; Kiviranta, J; Vuorela, H; Hiltunen, R

1999-12-01

406

Time-course and dose-response relationships of imperatorin in the mouse maximal electroshock seizure threshold model.  

PubMed

This study was designed to evaluate the anticonvulsant effects of imperatorin (a furanocoumarin isolated from fruits of Angelica archangelica) in the mouse maximal electroshock seizure threshold model. The threshold for electroconvulsions in mice was determined at several times: 15, 30, 60 and 120 min after i.p. administration of imperatorin at increasing doses of 10, 20, 30, 40, 50 and 100 mg/kg. The evaluation of time-course relationship for imperatorin in the maximal electroshock seizure threshold test revealed that the agent produced its maximum antielectroshock action at 30 min after its i.p. administration. In this case, imperatorin at doses of 50 and 100 mg/kg significantly raised the threshold for electroconvulsions in mice by 38 and 68% (P<0.05 and P<0.001), respectively. The antiseizure effects produced by imperatorin at 15, 60 and 120 min after its systemic (i.p.) administration were less expressed than those observed for imperatorin injected 30 min before the maximal electroshock seizure threshold test. Based on this study, one can conclude that imperatorin produces the anticonvulsant effect in the maximal electroshock seizure threshold test in a dose-dependent manner. PMID:17602770

Luszczki, Jarogniew J; Glowniak, Kazimierz; Czuczwar, Stanislaw J

2007-09-01

407

Effect of a novel botanical agent Drynol Cibotin on human osteoblast cells and implications for osteoporosis: promotion of cell growth, calcium uptake and collagen production.  

PubMed

Osteoporosis is a widespread problem afflicting millions of people. Drynol Cibotinis is a newly developed proprietary botanical combination of eight botanicals including Angelica sinensis, Glycine max, Wild yam, Ligustrum lucidum, Astragalus membranaceus, Cuscuta chinensis, Psoraleae corylifoliae, and Drynaria fortune. Each of the botanicals has been used in traditional Chinese medicine to treat osteoporosis. The effect of Drynol Cibotinis, with the specific combination of these anti-osteoporosis botanicals for promoting bone growth, was examined in this study. The effects of Drynol Cibotin on cell growth, apoptosis, cell spreading, calcium uptake and production of bone matrix proteins Collagen I and Laminin B2 on human osteoblast cells were assessed by BrdU incorporation, TUNEL assay, cell staining, intracellular Ca2+ measurement and Western blot analysis. The results showed that Drynol Cibotin significantly increased cell proliferation and inhibited apoptosis in osteoblasts (P < 0.01). In addition, Drynol Cibotin was found to promote cell spreading and greatly increase calcium uptake both instantaneously and in the long term (P < 0.01). Furthermore, Drynol Cibotin significantly increased production of two key extracellular matrix proteins in bone cells: Collagen I and Laminin B2. These results indicate that Drynol Cibotin alone or in combination with amino acids and vitamins may have prophylactic potentials in osteoporosis. PMID:19953582

Wegiel, Barbara; Persson, Jenny L

2010-06-01

408

Evaluation of free hydroxyl radical scavenging activities of some Chinese herbs by capillary zone electrophoresis with amperometric detection.  

PubMed

Due to the severe damage caused by free hydroxyl radicals (OH.) to cells and tissues, there is much interest in finding and studying effective and non-toxic OH. scavengers, including traditional Chinese herbs. In this paper, the simple and highly-sensitive technique of capillary zone electrophoresis with amperometric detection (CZE-AD) was used to study the OH. scavenging activities of aqueous extracts from some traditional Chinese herbs. Salicylic acid (SAL) was used as an OH. trap, and the content of OH. could be determined by assaying their products, 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihydroxybenzoic acid (2,5-DHBA). The optimum conditions for CZE-AD for the determination of 2,3-DHBA and 2,5-DHBA were explored. The linearity ranges of 2,3-DHBA and 2,5-DHBA were 1.0 x10(-7) approximately 1.0 x10(-4) mol L(-1), and their detection limits were as low as 2 x 10(-8) mol L(-1), which were much better than the CE-UV method often used. The traditional Chinese herbs studied included Radix angelicae sinensis, Rhizoma coptidis, Ligustrum lucidum, Ligusticum wallichii, Radices glycyrrhizae and Semen plantaginis. The experiments showed that the aqueous extracts from all of the above traditional Chinese herds had free OH. scavenging activities, although to different degrees. PMID:14985912

Li, Hui; Wang, Qingjiang

2004-04-01

409

Molecular screening of Chinese medicinal plants for progestogenic and anti-progestogenic activity.  

PubMed

Estrogen and progestins have adverse effects, and many of these adverse effects are caused by progestins. Due to this, many women choose to use botanical alternatives for hormone replacement therapy, which does not trigger steroidogenic properties. Therefore, it is necessary to screen these herbs for progestogenic and anti-progestogenic properties. Extract of 13 Chinese medicinal plants were analysed for progestogenic and anti-progestogenic activities by using progesterone response element-driven luciferase reporter gene bioassay. MTT assay was carried out to investigate the cytotoxic effect of herb extract on PAE cells. Among the 13 herbs, Dipsacus asperoides extract exhibited progestogenic activity, and 10 species - Cortex eucommiae, Folium artemisiae argyi, Glycyrrhiza uralensis, Angelica sinensis, Atractylodes macrocephala koidz, Scutellaria baicalensis, Cuscuta chinensis, Euscaphis japonica, Ailanthus altissima, and Dioscorea opposita - were recognized to have anti-progestogenic like activities. Extract of Dipsacus asperoides demonstrated dose-dependent progestogenic activity, and the progestogenic activity of 100 (mu)g/mL extracts was equivalent to 31.45 ng/mL progesterone activity. Herbs extracts that exhibited anti-progestogenic-like activity also inhibited the 314.46 ng/mL progesterone activity in a dose-response manner. None of the herb extracts shown significant toxic effect on PAE cells at 40-100 (mu)g/mL compared to control. This discovery will aid selection of suitable herbs for hormone replacement therapy. PMID:24845509

Ahmed, Hm Manir; Yeh, Jan-Ying; Tang, Yi-Chia; Cheng, Winston Teng-Kuei; Ou, Bor-Rung

2014-06-01

410

Extraction, separation and quantitative structure-retention relationship modeling of essential oils in three herbs.  

PubMed

The essential oils extracted from three kinds of herbs were separated by a 5% phenylmethyl silicone (DB-5MS) bonded phase fused-silica capillary column and identified by MS. Seventy-four of the compounds identified were selected as origin data, and their chemical structure and gas chromatographic retention times (RT) were performed to build a quantitative structure-retention relationship model by genetic algorithm and multiple linear regressions analysis. The predictive ability of the model was verified by internal validation (leave-one-out, fivefold, cross-validation and Y-scrambling). As for external validation, the model was also applied to predict the gas chromatographic RT of the 14 volatile compounds not used for model development from essential oil of Radix angelicae sinensis. The applicability domain was checked by the leverage approach to verify prediction reliability. The results obtained using several validations indicated that the best quantitative structure-retention relationship model was robust and satisfactory, could provide a feasible and effective tool for predicting the gas chromatographic RT of volatile compounds and could be also applied to help in identifying the compound with the same gas chromatographic RT. PMID:20506431

Wei, Yuhui; Xi, Lili; Chen, Dongxia; Wu, Xin'an; Liu, Huanxiang; Yao, Xiaojun

2010-07-01

411

Screening of 20 commonly used Iranian traditional medicinal plants against urease.  

PubMed

Infection with Helicobacter pyloriis the most common cause of stomach and duodenal ulcers. About more than 80 % of people are infected with H. pylori in developing countries. H. pylori uses urease enzyme product "ammonia" in order to neutralize and protect itself from the stomach acidic condition and urease enzyme activity has been shown to be essential to the colonization of H. pylori. Inhibitory activity of 20 traditional medicinal plants were examined and evaluated against Jack bean urease activity by Berthelot reaction to obtains natural sources of urease inhibitors. Each herb was extracted using 80% aqueous methanol, then tested its IC50 value was determined. Eight of the whole 20 studied plants crude extracts were found the most effective with IC50 values of less than 100 ?g/mL including Laurus nobilis, Zingiber officinale, Nigella sativa, Angelica archangelica, Acorus calamus, Allium sativum,Curcuma longa, and Citrus aurantium extracts, from which most potent urease inhibitory was observed for Zingiber officinale, Laurus nobilis, and Nigella sativa with IC50 values of 48.54, 48.69 and 59.10 ?g/mL, respectively. PMID:24711846

Biglar, Mahmood; Sufi, Hessameddin; Bagherzadeh, Kowsar; Amanlou, Massoud; Mojab, Faraz

2014-01-01

412

Study on structural and spectral properties of isobavachalcone and 4-hydroxyderricin by computational method.  

PubMed

Isobavachalcone and 4-hydroxyderricin, two major chalcone constituents isolated from the roots of Angelica keiskei KOIDZUMI, exhibit numerous biological activities. Quantum chemical methods have been employed to investigate their structural and spectral properties. The ground state structures were optimized using density functional B3LYP method with 6-311G (d, p) basis set in both gas and solvent phases. Based on the optimized geometries, the harmonic vibrational frequency, the (1)H and (13)C nuclear magnetic resonance (NMR) chemical shift using the GIAO method were calculated at the same level of theory, with the aim of verifying the experimental values. Results reveal that B3LYP has been a good method to study their vibrational spectroscopic and NMR spectral properties of the two chalcones. The electronic absorption spectra were calculated using the time-dependent density functional theory (TDDFT) method. The solvent polarity effects were considered and calculated using the polarizable continuum model (PCM). Results also show that substitutions of different electron donating groups can alter the absorption properties and shift the spectra to a higher wavelength region. PMID:24607475

Rong, Yuzhi; Wu, Jinhong; Liu, Xing; Zhao, Bo; Wang, Zhengwu

2014-05-21

413

Immunostimulatory effects of artificial feed supplemented with a Chinese herbal mixture on Oreochromis niloticus against Aeromonas hydrophila.  

PubMed

The effects of a Chinese herbal mixture (CHM) composed of astragalus, angelica, hawthorn, Licorice root and honeysuckle on immune responses and disease resistant of Nile tilapia (Oreochromis niloticus GIFT strain) were investigated in present study. Fish were fed diets containing 0 (control), 0.5%, 1.0%, 1.5% or 2.0% CHM (w/w) for 4 weeks. And series of immune parameters including lysozyme, cytokine genes TNF-? and IL-1?, superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA) were measured during test period. After four weeks of feeding, fish were infected with Aeromonas hydrophila and mortalities were recorded. Results of this study showed that feeding Nile tilapia with CHM-supplementation diet stimulated lysozyme activity, SOD activity and POD activity in serum, induced TNF-? and IL-1? mRNA expression in head kidney and spleen, but decreased serum MDA content. All CHM-supplemental groups showed reduced mortalities following A. hydrophila infection compared with the group fed the control diet. These results suggested that this CHM can be applied as a tilapia feed supplement to elevate fish immunity and disease resistance against A. hydrophila. PMID:24925761

Tang, Jufen; Cai, Jia; Liu, Ran; Wang, Jiamin; Lu, Yishan; Wu, Zaohe; Jian, Jichang

2014-08-01

414

Antioxidant properties of selected Oriental non-culinary/nutraceutical herb extracts as evaluated in raw and cooked meat.  

PubMed

Ethanol extracts of white peony (WP), red peony (RP), sappanwood (SW), Moutan peony (MP), rehmania (RE) or angelica (AN) were individually added to ground goat meat at 0.5-2.0% (g dry extract/100 g final meat sample), and raw and cooked samples were aerobically refrigerated for 0, 3 or 6 days. These extracts and rosemary extract (RO) were also individually added to salted or unsalted ground beef at 0.01-0.25% and refrigerated as raw or cooked patties. WP, RP, RE, SW and MP markedly reduced (P<0.05) lipid oxidation in cooked-stored goat meat. With 0.25% of WP, RP, SW, MP or RO in beef, lipid oxidation during storage was minimal in raw and cooked patties (plain or salted); raw patty redness values at day 6 were higher (P<0.05) for SW, WP, RP or MP than RO treatment or the control. At 0.01%, SW was more antioxidative (P<0.05) than the other extracts. PMID:22063277

Han, J; Rhee, K S

2005-05-01

415

Imperatorin sustained-release tablets: In vitro and pharmacokinetic studies.  

PubMed

We prepared and evaluated imperatorin (IMP) sustained-release tablets. IMP is an active compound in Angelica dahuricae, a Chinese herbal medicine. We used different polymers, such as hydroxypropyl methylcellulose (HPMC K4M, K15M, and K100M), carbopol 934P, sodium carboxymethyl cellulose (CMC-Na), and their combinations to prepare the matrix tablets and achieve the desired sustained release profile. The in vitro release profiles of these formulations were examined and fit to various kinetic release models. We also tested the effects of polymer combination ratios on the in vitro release rate. In vivo studies were performed for the optimized formulation in six beagle dogs, and pharmacokinetic parameters were compared with plain IMP tablets. IMP sustained-release tablets exhibited a more sustained plasma concentration than the plain tablets, with a relative bioavailability of 127.25%. The in vitro releases rates and in vivo absorption correlated for the initial 8 hours. These results demonstrate that the sustained-release tablet system can effectively control the release of IMP. PMID:20803124

Pan, Jingjing; Lu, Wen; Li, Changhui; Wang, Sicen; He, Langchong

2010-08-01

416

Quality Control of Danggui Buxue Tang, a Traditional Chinese Medicine Decoction, by 1H-NMR Metabolic Profiling  

PubMed Central

Danggui Buxue Tang (DBT) is one of the simplest traditional Chinese medicine (TCM) decoctions, first described in China in 1247 AD. DBT is composed of 2 herbs, Astragali Radix (AR) and Angelica Sinensis Radix (ASR), boiled together in a 5?:?1 ratio. Clinically, DBT is prescribed to women as a remedy for menopausal symptoms. Here, H-NMR metabolic profiling was conducted for DBT and the water extracts of AR or ASR, to evaluate the potential of this chemical profiling method for quality control of the herbal decoction. Principal component analysis (PCA) showed that DBT could be readily distinguished from the water extracts of its constituent herbs by the metabolic profiles. More interestingly, the metabolic profile of DBT was not a simple sum of that of AR and ASR. Asparagine was found at significantly higher concentration in DBT than that in either AR or ASR extract, contributing mainly to the discrimination of DBT sample. In addition, we employed the same method to profile a commercial DBT powder, verifying its authenticity as compared to our prepared DBT. This study is the first to employ H-NMR metabolic profiling for the quality control of traditional Chinese medicine decoctions.

Zhang, Wendy L.; Cheung, Chi Yuen; Tsim, Karl W. K.

2014-01-01

417

Simultaneous Determination of 11 Major Components in Palmul-tang by HPLC-DAD and LC-MS-MS.  

PubMed

Palmul-tang, a traditional herbal medicine, is composed of eight herbs (Ginseng radix, Glycyrrhizae radix, Hoelen, Atractylodis rhizoma, Angelicae gigantis radix, Cnidii rhizoma, Paeoniae radix and Rehmanniae radix) and exhibits various bioactivities, including antiallergic and antitumor effects. In this study, an effective, reliable and accurate high-performance liquid chromatography method has been developed for the simultaneous determination of 11 marker components in Palmul-tang: hydroxymethylfurfural, albiflorin, paeoniflorin, ferulic acid, nodakenin, ginsenoside Rg1, decursinol, glycyrrhizin, 6-gingerol, ginsenoside Rg3 and decursin. All calibration curves of the 11 components indicated excellent linearity (correlation coefficient > 0.9997) within the test range. The limits of detection and quantification of each component were in the ranges of 0.08-1.03 and 0.23-3.11 µg/mL, respectively. The intra-day and inter-day relative standard deviation values were within 1.65 and 2.71%, respectively. The mean recovery values were 94.49 to 101.10%. The established method was successfully applied to the simultaneous determination of 11 major components in 12 commercial samples of Palmul-tang. The developed analytical method is simple and suitable for the quality control of Palmul-tang. PMID:23741048

Lee, Bohyoung; Weon, Jin Bae; Yun, Bo-Ra; Lee, Jiwoo; Eom, Min Rye; Ma, Choong Je

2014-07-01

418

Hydrolysis of Glycosidic Flavonoids during the Preparation of Danggui Buxue Tang: An Outcome of Moderate Boiling of Chinese Herbal Mixture  

PubMed Central

Chemical change during boiling of herbal mixture is a puzzle. By using Danggui Buxue Tang (DBT), a herbal decoction that contains Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), we developed a model in analyzing the hydrolysis of flavonoid glycosides during the boiling of herbal mixture in water. A proper preparation of DBT is of great benefit to the complete extraction of bioactive ingredients. Boiling of DBT in water increased the solubility of AR-derived astragaloside IV, calycosin, formononetin, calycosin-7-O-?-D-glucoside, and ononin in a time- and temperature-dependent manner: the amounts of these chemicals reached a peak at 2?h. The glycosidic resides of AR, calycosin-7-O-?-D-glucoside, and ononin could be hydrolyzed during the moderate boiling process to form calycosin and formononetin, respectively. The hydrolysis efficiency was strongly affected by pH, temperature, and amount of herbs. Interestingly, the preheated herbs were not able to show this hydrolytic activity. The current results supported the rationality of ancient preparation of DBT in boiling water by moderate heat.

Zhang, Wendy Li; Chen, Jian-Ping; Lam, Kelly Yin-Ching; Zhan, Janis Ya-Xian; Yao, Ping; Dong, Tina Ting-Xia; Tsim, Karl Wah-Keung

2014-01-01

419

Nam Con Son Basin  

SciTech Connect

The Nam Con Son basin is the largest oil and gas bearing basin in Vietnam, and has a number of producing fields. The history of studies in the basin can be divided into four periods: Pre-1975, 1976-1980, 1981-1989, and 1990-present. A number of oil companies have carried out geological and geophysical studies and conducted drilling activities in the basin. These include ONGC, Enterprise Oil, BP, Shell, Petro-Canada, IPL, Lasmo, etc. Pre-Tertiary formations comprise quartz diorites, granodiorites, and metamorphic rocks of Mesozoic age. Cenozoic rocks include those of the Cau Formation (Oligocene and older), Dua Formation (lower Miocene), Thong-Mang Cau Formation (middle Miocene), Nam Con Son Formation (upper Miocene) and Bien Dong Formation (Pliocene-Quaternary). The basement is composed of pre-Cenozoic formations. Three fault systems are evident in the basin: north-south fault system, northeast-southwest fault system, and east-west fault system. Four tectonic zones can also be distinguished: western differentiated zone, northern differentiated zone, Dua-Natuna high zone, and eastern trough zone.

Tin, N.T.; Ty, N.D.; Hung, L.T.

1994-07-01

420

Accelerated transport and growth with symmetrized dynamics.  

PubMed

In this paper we consider a model of accelerated dynamics with the rules modified from those of the recently proposed [Dong et al., Phys. Rev. Lett. 109, 130602 (2012)] accelerated exclusion process (AEP) such that particle-vacancy symmetry is restored to facilitate a mapping to a solid-on-solid growth model in 1+1 dimensions. In addition to kicking a particle ahead of the moving particle, as in the AEP, in our model another particle from behind is drawn, provided it is within the "distance of interaction" denoted by ?(max). We call our model the doubly accelerated exclusion process (DAEP). We observe accelerated transport and interface growth and widening of the cluster size distribution for cluster sizes above ?(max), when compared with the ordinary totally asymmetric exclusion process (TASEP). We also characterize the difference between the TASEP, AEP, and DAEP by computing a "staggered" order parameter, which reveals the local order in the steady state. This order in part explains the behavior of the particle current as a function of density. The differences of the steady states are also reflected by the behavior of the temporal and spatial correlation functions in the interface picture. PMID:24483416

Merikoski, Juha

2013-12-01

421

On representations of conformal field theories and the construction of orbifolds  

NASA Astrophysics Data System (ADS)

We consider representations of meromorphic bosonic chiral conformal field theories and demonstrate that such a representation is completely specified by a state within the theory. The necessary and sufficient conditions upon this state are derived and, because of their form, we show that we may extend the representation to a representation of a suitable larger conformal field theory. In particular, we apply this procedure to the (untwisted) lattice conformal field theories (i.e. corresponding to the propagation of a bosonic string on a torus), and deduce that Dong's proof of the uniqueness of the twisted representation for the reflection-twisted projection of the Leech lattice conformal field theory generalises to an arbitrary even (self-dual) lattice. As a consequence, we see that the reflection-twisted lattice theories of Dolan, Goddard and Montague are truly self-dual, extending the analogies with the theories of lattices and codes which were being pursued. Some comments are also made on the general concept of the definition of an orbifold of a conformal field theory in relation to this point of view.

Montague, P. S.