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Sample records for em portugal fim

  1. Portugal.

    ERIC Educational Resources Information Center

    Eckles, David; Toro, Leonor, Ed.

    Brief information is provided on the region, climate, agriculture and industry of 10 provinces in Portugal: Tras-os-Montes e Alto Douro, located in Portugal's northeastern region; Beira Alta, the province with Portugal's highest elevation point; Beira Baixa, one of Portugal's poorest regions; Beira Litoral, located in central Portugal along the…

  2. Portugal.

    PubMed

    1985-03-01

    Portugal's 1983 population of 10.04 million was growing at .7%/year. 97% were Roman Catholic, 80% were literate, and 4.1 million were in the labor force in 1984. Portugal is mountainous north of the Tagus River and consists of rolling plains in the central south. The Azores and Madeira islands belong to Portugal but enjoy considerable autonomy. Portugal's dependency of Macau on the Chinese coast is an autonomous entity under Portuguese administration. The former teritories of Goa and Portuguese Timor were annexed by India and Indonesia respectively in 1961 and 1976, and the former territories in Africa achieved independence in 1974 and 1975. Portugal, 1 of the oldest states in Europe, traces its modern history to A.D. 1140 when the 1st king was crowned. The approximate present day boundaries were secured in 1249. Portugal eventually became a massive colonial empire with territories in Africa and Latin America and outposts in the Far East. The Portuguese Republic replaced the monarchy in 1910. After some years of instability, Antonio Salazar became prime minister, and with his successor, ruled Portugal as an authoritarian "corporate" state for 42 years. An almost bloodless coup in 1974 led to establishment of a parliamentary democracy. The 4 main organs of national government are the presidency, the prime minister and council of ministers, the assembly of the republic, and the courts. Portugal's gross domestic product (GDP) in 1984 was $19.4 billion, and per capita income was $1930. After almost a decade of rapid growth, the economy declined in the mid 1970s due to disruption after the 1974 revolution and the large influx of refugees from Portugal's former colonies in Africa. Growth resumed in the late 1970s, but structural problems and an often adverse international economic climate have slowed progress. The proportion of the labor force in agriculture has dropped from 42% to 24.4% since 1960. Agricultural production now contributes only 8.8% to the GDP, and a

  3. Pre-School Education in Portugal = Educacao Pre-Escolar em Portugal. Report.

    ERIC Educational Resources Information Center

    Ministry of Education, Lisbon (Portugal).

    This report, containing both English and Portuguese text, describes the historical background and current state of preschool education systems in Portugal, and presents recommendations on guideline consolidation in the implementation of a common preschool education policy. Part 1 of the report, "Historical Background," discusses the social,…

  4. Crystal Structure of the FimD Usher Bound to its Cognate FimC-FimH Substrate

    SciTech Connect

    G Phan; H Remaut; T Wang; W Allen; K Pirker; A Lebedev; N Henderson; S Geibel; E Volkan; et al.

    2011-12-31

    Type 1 pili are the archetypal representative of a widespread class of adhesive multisubunit fibres in Gram-negative bacteria. During pilus assembly, subunits dock as chaperone-bound complexes to an usher, which catalyses their polymerization and mediates pilus translocation across the outer membrane. Here we report the crystal structure of the full-length FimD usher bound to the FimC-FimH chaperone-adhesin complex and that of the unbound form of the FimD translocation domain. The FimD-FimC-FimH structure shows FimH inserted inside the FimD 24-stranded {beta}-barrel translocation channel. FimC-FimH is held in place through interactions with the two carboxy-terminal periplasmic domains of FimD, a binding mode confirmed in solution by electron paramagnetic resonance spectroscopy. To accommodate FimH, the usher plug domain is displaced from the barrel lumen to the periplasm, concomitant with a marked conformational change in the {beta}-barrel. The amino-terminal domain of FimD is observed in an ideal position to catalyse incorporation of a newly recruited chaperone-subunit complex. The FimD-FimC-FimH structure provides unique insights into the pilus subunit incorporation cycle, and captures the first view of a protein transporter in the act of secreting its cognate substrate.

  5. Crystal structure of the FimD usher bound to its cognate FimC-FimH substrate

    SciTech Connect

    Phan, G.; Li, H.; Remaut, H.; Wang, T.; Allen, W. J.; Pirker, K. F.; Lebedev, A.; Henderson, N. S.; Geibel, S.; Volkan, E.; Yan, J.; Kunze, M. B. A.; Pinkner, J. S.; Ford, B.; Kay, C. W. M.; Hultgren, S. J.; Thanassi, D. G.; Waksman, G.

    2011-06-02

    Type 1 pili are the archetypal representative of a widespread class of adhesive multisubunit fibres in Gram-negative bacteria. During pilus assembly, subunits dock as chaperone-bound complexes to an usher, which catalyses their polymerization and mediates pilus translocation across the outer membrane. Here we report the crystal structure of the full-length FimD usher bound to the FimC-FimH chaperone-adhesin complex and that of the unbound form of the FimD translocation domain. The FimD-FimC-FimH structure shows FimH inserted inside the FimD 24-stranded {beta}-barrel translocation channel. FimC-FimH is held in place through interactions with the two carboxy-terminal periplasmic domains of FimD, a binding mode confirmed in solution by electron paramagnetic resonance spectroscopy. To accommodate FimH, the usher plug domain is displaced from the barrel lumen to the periplasm, concomitant with a marked conformational change in the {beta}-barrel. The amino-terminal domain of FimD is observed in an ideal position to catalyse incorporation of a newly recruited chaperone-subunit complex. The FimD-FimC-FimH structure provides unique insights into the pilus subunit incorporation cycle, and captures the first view of a protein transporter in the act of secreting its cognate substrate.

  6. Crystal structure of the FimD usher bound to its cognate FimC:FimH substrate

    PubMed Central

    Phan, Gilles; Remaut, Han; Wang, Tao; Allen, William J.; Pirker, Katharina F.; Lebedev, Andrey; Henderson, Nadine S.; Geibel, Sebastian; Volkan, Ender; Yan, Jun; Kunze, Micha B.A.; Pinkner, Jerome S.; Ford, Bradley; Kay, Christopher W. M.; Li, Huilin; Hultgren, Scott; Thanassi, David G.; Waksman, Gabriel

    2011-01-01

    Type 1 pili are the archetypal representative of a widespread class of adhesive multisubunit fibres in Gram-negative bacteria. During pilus assembly, subunits dock as chaperone-bound complexes to an usher, which catalyzes their polymerization and mediates pilus translocation across the outer membrane. We report the crystal structure of the full-length FimD usher bound to the FimC:FimH chaperone:adhesin complex and that of the unbound form of the FimD translocation domain. The FimD:FimC:FimH structure shows FimH inserted inside the FimD 24-stranded β-barrel translocation channel. FimC:FimH is held in place through interactions with the two C-terminal periplasmic domains of FimD, a binding mode confirmed in solution by electron paramagnetic resonance spectroscopy. To accommodate FimH, the usher plug domain is displaced from the barrel lumen to the periplasm, concomitant with a dramatic conformational change in the β-barrel. The N-terminal domain of FimD is observed in an ideal position to catalyse incorporation of a newly recruited chaperone:subunit complex. The FimD:FimC:FimH structure provides unique insights into the pilus subunit incorporation cycle, and captures the first view of a protein transporter in the act of secreting its cognate substrate. PMID:21637253

  7. FIM Levels as Ordinal Categories.

    ERIC Educational Resources Information Center

    Linacre, John M.

    2000-01-01

    Discusses levels of the Functional Independence Measure (FIM) as ordinal categories. Presents guidelines developed through the Rasch model that prompt an analyst to investigate whether rating categories produce observations on which meaningful measurement and inference about patient status can be based. (SLD)

  8. Early Childhood Education and Care Policy in Portugal = A Educacao Pre-Escolar e os Cuidados para a Infancia em Portugal.

    ERIC Educational Resources Information Center

    Ministry of Education, Lisbon (Portugal).

    Based on the view that the rapid expansion and development of preschool education requires careful scrutiny of both educational policy and practices, this book presents information on current early childhood education and care policy in Portugal. Section 1 of the book provides a historical framework for the development of early childhood education…

  9. Evaluating the FONE FIM: Part I. Construct Validity.

    ERIC Educational Resources Information Center

    Chang, Wei-Ching; Slaughter, Susan; Cartwright, Deborah; Chan, Chetwyn

    1997-01-01

    Rasch analysis, often used in educational research, was used to evaluate the Motor component of the FONE FIM, the telephone version of the Functional Independence Measure (FIM) (validated by T. Dodds, and others, 1993) with 132 geriatric patients. Rasch difficulty measures demonstrated the similarity of the phone and observational FIM measures.…

  10. Positively selected FimH residues enhance virulence during urinary tract infection by altering FimH conformation

    PubMed Central

    Schwartz, Drew J.; Kalas, Vasilios; Pinkner, Jerome S.; Chen, Swaine L.; Spaulding, Caitlin N.; Dodson, Karen W.; Hultgren, Scott J.

    2013-01-01

    Chaperone–usher pathway pili are a widespread family of extracellular, Gram-negative bacterial fibers with important roles in bacterial pathogenesis. Type 1 pili are important virulence factors in uropathogenic Escherichia coli (UPEC), which cause the majority of urinary tract infections (UTI). FimH, the type 1 adhesin, binds mannosylated glycoproteins on the surface of human and murine bladder cells, facilitating bacterial colonization, invasion, and formation of biofilm-like intracellular bacterial communities. The mannose-binding pocket of FimH is invariant among UPEC. We discovered that pathoadaptive alleles of FimH with variant residues outside the binding pocket affect FimH-mediated acute and chronic pathogenesis of two commonly studied UPEC strains, UTI89 and CFT073. In vitro binding studies revealed that, whereas all pathoadaptive variants tested displayed the same high affinity for mannose when bound by the chaperone FimC, affinities varied when FimH was incorporated into pilus tip-like, FimCGH complexes. Structural studies have shown that FimH adopts an elongated conformation when complexed with FimC, but, when incorporated into the pilus tip, FimH can adopt a compact conformation. We hypothesize that the propensity of FimH to adopt the elongated conformation in the tip corresponds to its mannose binding affinity. Interestingly, FimH variants, which maintain a high-affinity conformation in the FimCGH tip-like structure, were attenuated during chronic bladder infection, implying that FimH’s ability to switch between conformations is important in pathogenesis. Our studies argue that positively selected residues modulate fitness during UTI by affecting FimH conformation and function, providing an example of evolutionary tuning of structural dynamics impacting in vivo survival. PMID:24003161

  11. Bright stars observed by FIMS/SPEAR

    NASA Astrophysics Data System (ADS)

    Jo, Young-Soo; Seon, Kwang-Il; Min, Kyoung-Wook; Choi, Yeon-Ju; Lim, Tae-Ho; Lim, Yeo-Myeong; Edelstein, Jerry; Han, Wonyong

    2016-02-01

    In this paper, we present a catalogue of the spectra of bright stars observed during the sky survey using the Far-Ultraviolet Imaging Spectrograph (FIMS), which was designed primarily to observe diffuse emissions. By carefully eliminating the contamination from the diffuse background, we obtain the spectra of 70 bright stars observed for the first time with a spectral resolution of 2-3 Å over the wavelength of 1370-1710 Å. The far-ultraviolet spectra of an additional 139 stars are also extracted with a better spectral resolution and/or higher reliability than those of the previous observations. The stellar spectral type of the stars presented in the catalogue spans from O9 to A3. The method of spectral extraction of the bright stars is validated by comparing the spectra of 323 stars with those of the International Ultraviolet Explorer (IUE) observations.

  12. Porphyromonas gingivalis Fim-A genotype distribution among Colombians

    PubMed Central

    Jaramillo, Adriana; Parra, Beatriz; Botero, Javier Enrique; Contreras, Adolfo

    2015-01-01

    Introduction: Porphyromonas gingivalis is associated with periodontitis and exhibit a wide array of virulence factors, including fimbriae which is encoded by the FimA gene representing six known genotypes. Objetive: To identify FimA genotypes of P. gingivalis in subjects from Cali-Colombia, including the co-infection with Aggregatibacter actinomycetemcomitans, Treponema denticola, and Tannerella forsythia. Methods: Subgingival samples were collected from 151 people exhibiting diverse periodontal condition. The occurrence of P. gingivalis, FimA genotypes and other bacteria was determined by PCR. Results: P. gingivalis was positive in 85 patients. Genotype FimA II was more prevalent without reach significant differences among study groups (54.3%), FimA IV was also prevalent in gingivitis (13.0%). A high correlation (p= 0.000) was found among P. gingivalis, T. denticola, and T. forsythia co-infection. The FimA II genotype correlated with concomitant detection of T. denticola and T. forsythia. Conclusions: Porphyromonas gingivalis was high even in the healthy group at the study population. A trend toward a greater frequency of FimA II genotype in patients with moderate and severe periodontitis was determined. The FimA II genotype was also associated with increased pocket depth, greater loss of attachment level, and patients co-infected with T. denticola and T. forsythia. PMID:26600627

  13. FimY of Salmonella enterica serovar Typhimurium functions as a DNA-binding protein and binds the fimZ promoter.

    PubMed

    Wang, Ke-Chuan; Hsu, Yuan-Hsun; Huang, Yi-Ning; Lin, Jiunn-Horng; Yeh, Kuang-Sheng

    2014-01-01

    Salmonella enterica serovar Typhimurium produces type 1 fimbriae with binding specificity to mannose residues. Elements involved in fimbrial structural biosynthesis, transport, and regulation are encoded by the fim gene cluster. FimZ, FimY, FimW, STM0551, and an arginine transfer RNA (fimU) were previously demonstrated to regulate fimbrial expression. The amino acid sequences of the C-terminal portion of FimY revealed similarity with those of LuxR-like proteins. Electrophoretic mobility shift assays indicated that FimY possessed DNA-binding capacity and bound a 605-bp DNA fragment spanning the intergenic region between fimY and fimZ, while a FimY protein harboring a double mutation in the C-terminal helix-turn-helix region containing a glycine (G) to aspartate (D) substitution at residue 189 and isoleucine (I) to lysine (K) substitution at residue 195 lost its ability to bind this DNA fragment. A lux box sequence (5'-TCTGTTATTACATAACAAATACT-3') within the fimZ promoter was required for binding. None of the DNA fragments derived from the promoters for fimA, fimY, or fimW was shifted by FimY. Pull-down assays showed that there were physical protein/protein interactions between FimY and FimZ. We propose that in the regulatory circuit of type 1 fimbriae, FimY functions as a DNA-binding protein to activate fimZ, and a FimY-FimZ protein complex may form to regulate other fim genes. Confirming these proposals requires further study. PMID:24462182

  14. Comprehensive mutagenesis of the fimS promoter regulatory switch reveals novel regulation of type 1 pili in uropathogenic Escherichia coli

    PubMed Central

    Zhang, Huibin; Susanto, Teodorus T.; Wan, Yue

    2016-01-01

    Type 1 pili (T1P) are major virulence factors for uropathogenic Escherichia coli (UPEC), which cause both acute and recurrent urinary tract infections. T1P expression therefore is of direct relevance for disease. T1P are phase variable (both piliated and nonpiliated bacteria exist in a clonal population) and are controlled by an invertible DNA switch (fimS), which contains the promoter for the fim operon encoding T1P. Inversion of fimS is stochastic but may be biased by environmental conditions and other signals that ultimately converge at fimS itself. Previous studies of fimS sequences important for T1P phase variation have focused on laboratory-adapted E. coli strains and have been limited in the number of mutations or by alteration of the fimS genomic context. We surmounted these limitations by using saturating genomic mutagenesis of fimS coupled with accurate sequencing to detect both mutations and phase status simultaneously. In addition to the sequences known to be important for biasing fimS inversion, our method also identifies a previously unknown pair of 5′ UTR inverted repeats that act by altering the relative fimA levels to control phase variation. Thus we have uncovered an additional layer of T1P regulation potentially impacting virulence and the coordinate expression of multiple pilus systems. PMID:27035967

  15. Allosteric Coupling in the Bacterial Adhesive Protein FimH*

    PubMed Central

    Rodriguez, Victoria B.; Kidd, Brian A.; Interlandi, Gianluca; Tchesnokova, Veronika; Sokurenko, Evgeni V.; Thomas, Wendy E.

    2013-01-01

    The protein FimH is expressed by the majority of commensal and uropathogenic strains of Escherichia coli on the tips of type 1 fimbriae and mediates adhesion via a catch bond to its ligand mannose. Crystal structures of FimH show an allosteric conformational change, but it remains unclear whether all of the observed structural differences are part of the allosteric mechanism. Here we use the protein structural analysis tool RosettaDesign combined with human insight to identify and synthesize 10 mutations in four regions that we predicted would stabilize one of the conformations of that region. The function of each variant was characterized by measuring binding to the ligand mannose, whereas the allosteric state was determined using a conformation-specific monoclonal antibody. These studies demonstrated that each region investigated was indeed part of the FimH allosteric mechanism. However, the studies strongly suggested that some regions were more tightly coupled to mannose binding and others to antibody binding. In addition, we identified many FimH variants that appear locked in the low affinity state. Knowledge of regulatory sites outside the active and effector sites as well as the ability to make FimH variants locked in the low affinity state may be crucial to the future development of novel antiadhesive and antimicrobial therapies using allosteric regulation to inhibit FimH. PMID:23821547

  16. Family participation in determination of FIM and Barthel indices.

    PubMed

    Kapidzić-Duraković, Suada; Zonić-Imamović, Maida; Mulaosmanović, Ahmet

    2006-05-01

    Functional Independence Measure (FIM) is a basic indicator of severity of disability, and is used for measurement of functional independence. The aim of this paper is to present our initial experiences in the application of FIM. It needs to be noted that we have had an incomplete team in implementation of this project, and also that we have involved the patients and their families in the measurement of FIM. We selected a small sample of 23 patients, and measured their FIM and Barthel index both at the admission and discharge from the hospital. We do accept that this sample may be too small for the purposes of scientific verification. Therefore, we have included patients and their family members in the process of FIM measurement, along with the team members including a physiatrist, physiotherapist, nurse, and a help-nurse. Most of the subjects included in this project were patients with hemiplegia after a stroke (65.21 %), followed by quadriplegic patients (17.39 %), and patients with paraplegia (17.39 %). Gender structure shows 47.82 % male and 52.17 % female patients. The best progress (an increase in score from 4 to 53 points) in FIM was observed in patients with hemiplegia, and in Barthel index in patients with quadriplegia (an increase in score from 0 to 84). The differences are not statistically significant (p>0.05). The patients with hemiplegia were hospitalized in our clinic for the shortest period of time (around one month). Average rehabilitation time in our clinic for paraplegic patients was around 3 months, and for quadriplegic patients around 4 months (including early rehabilitation). We established that the patients who actively participated in FIM measurement had more control over their own medical assistance, and this participation had a supportive effect on patients. Their participation in FIM measurement needed constant encouragement. In order to make our claim that the FIM is a good supportive method as objective as possible, and in order to

  17. 3D sensitivity of 6-electrode Focused Impedance Method (FIM)

    NASA Astrophysics Data System (ADS)

    Masum Iquebal, A. H.; Siddique-e Rabbani, K.

    2010-04-01

    The present work was taken up to have an understanding of the depth sensitivity of the 6 electrode FIM developed by our laboratory earlier, so that it may be applied judiciously for the measurement of organs in 3D, with electrodes on the skin surface. For a fixed electrode geometry sensitivity is expected to depend on the depth, size and conductivity of the target object. With current electrodes 18 cm apart and potential electrodes 5 cm apart, depth sensitivity of spherical conductors, insulators and of pieces of potato of different diameters were measured. The sensitivity dropped sharply with depth gradually leveling off to background, and objects could be sensed down to a depth of about twice their diameters. The sensitivity at a certain depth increases almost linearly with volume for objects with the same conductivity. Thus these results increase confidence in the use of FIM for studying organs at depths of the body.

  18. Lessons from Portugal

    ERIC Educational Resources Information Center

    Harry, Vickie; Mechling, Ken

    2010-01-01

    Portugal has long been touted as a popular destination for savvy travelers, but now educators are touting it as something else--a professional development (PD) powerhouse for inquiry-based science! This article describes Portugal's ongoing efforts to reform its country's elementary science education program through a project known as the…

  19. Tertiary Education in Portugal

    ERIC Educational Resources Information Center

    OECD Publishing (NJ3), 2008

    2008-01-01

    Portugal's tertiary education sector has expanded significantly over the last 20 years, but participation and overall educational attainment levels remain below European standards and enrolment rates have begun to decline. The OECD review recommends that Portugal's national government should focus on strategic direction and leave higher education…

  20. Global map based on the FIMS observations aboard STSAT-1

    NASA Astrophysics Data System (ADS)

    Min, Kyoung Wook

    2016-07-01

    The Far Ultraviolet Imaging Spectrograph (FIMS), a dual-channel instrument with 900 - 1150 A and 1350 - 1750 A passbands, was launched aboard the Korean microsatellite STSAT-1 on 2003 September 27. FIMS, with moderate spectral and angular resolutions while maintaining large fields of view, was optimized for observations of diffuse emissions such as those from hot gases in our Galaxy. About 70 percent of the sky was covered after 18 months of survey with sufficient exposure time for the long wavelength band. The dataset has been used to study the interaction between the hot gas and the cold component as well as the molecular hydrogen fluorescence emission for a variety of targets. Furthermore, it was successfully used to determine the optical properties of dust scattering and thereby the distances for several prominent clouds from the continuum observations. In the present paper, the global distribution of ion and molecular hydrogen lines will be presented although the coverage of the sky is somewhat limited. For example, topics such as the ion lines distribution in the Galactic halo regions and correlation of molecular hydrogen with dust or CO will be discussed.

  1. View FImP miracle (by scale invariance) à la self-interaction

    NASA Astrophysics Data System (ADS)

    Kang, Zhaofeng

    2015-12-01

    Combining feebly interacting massive particle (FIMP) dark matter (DM) with scale invariance (SI) leads to extremely light FIMP (thus the FImP) with FImP miracle, i.e., the mass and relic generations of FImP DM share the same dynamics. In this paper we show that due to the lightness of FImP, it, especially for a scalar FImP, can easily accommodate large DM self-interaction. For a fermionic FImP, such as the sterile neutrino, self-interaction additionally requires a mediator which is another FImP, a scalar boson with mass either much lighter or heavier than the FImP DM. DM self-interaction opens a new window to observe FImP (miracle), which does not leave traces in the conventional DM searches. As an example, FImP can account for the offsets between the centroid of DM halo and stars of galaxies recently observed in the galaxy cluster Abel 3827.

  2. The Conformational Variability of FimH: Which Conformation Represents the Therapeutic Target?

    PubMed

    Eris, Deniz; Preston, Roland C; Scharenberg, Meike; Hulliger, Fabian; Abgottspon, Daniela; Pang, Lijuan; Jiang, Xiaohua; Schwardt, Oliver; Ernst, Beat

    2016-06-01

    FimH is a bacterial lectin found at the tips of type 1 pili of uropathogenic Escherichia coli (UPEC). It mediates shear-enhanced adhesion to mannosylated surfaces. Binding of UPEC to urothelial cells initiates the infection cycle leading to urinary tract infections (UTIs). Antiadhesive glycomimetics based on α-d-mannopyranose offer an attractive alternative to the conventional antibiotic treatment because they do not induce a selection pressure and are therefore expected to have a reduced resistance potential. Genetic variation of the fimH gene in clinically isolated UPEC has been associated with distinct mannose binding phenotypes. For this reason, we investigated the mannose binding characteristics of four FimH variants with mannose-based ligands under static and hydrodynamic conditions. The selected FimH variants showed individually different binding behavior under both sets of conditions as a result of the conformational variability of FimH. Clinically relevant FimH variants typically exist in a dynamic conformational equilibrium. Additionally, we evaluated inhibitory potencies of four FimH antagonists representing different structural classes. Inhibitory potencies of three of the tested antagonists were dependent on the binding phenotype and hence on the conformational equilibrium of the FimH variant. However, the squarate derivative was the notable exception and inhibited FimH variants irrespective of their binding phenotype. Information on antagonist affinities towards various FimH variants has remained largely unconsidered despite being essential for successful antiadhesion therapy. PMID:26991759

  3. FIM/IAP/TEM studies of ion implanted nickel emitters

    SciTech Connect

    Walck, S.D.; Hren, J.J.

    1985-01-01

    Accurate depth profiling of implanted hydrogen and its isotopes in metals is extremely important. Field ion microscopy and atom-probe techniques provide the most accurate depth profiling analytical method of any available. In addition, they are extremely sensitive to hydrogen. This paper reports our early work on hydrogen trapping at defects in metals using the Field Ion Microscope/Imaging Atom Probe (FIM/IAP). Our results deal primarily with the control experiments required to overcome instrumental difficulties associated with in situ implantation and the influence of a high electric field. Transmission Electron Microscopy (TEM) has been used extensively to independently examine the influence of high electric fields on emitters. 11 references, 7 figures.

  4. FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes.

    PubMed Central

    Sokurenko, E V; Courtney, H S; Ohman, D E; Klemm, P; Hasty, D L

    1994-01-01

    We recently reported that the type 1-fimbriated Escherichia coli strains CSH-50 and HB101(pPKL4), both K-12 derivatives, have different patterns of adhesion to yeast mannan, human plasma fibronectin, and fibronectin derivatives, suggesting functional heterogeneity of type 1 fimbriae. In this report, we provide evidence that this functional heterogeneity is due to variations in the fimH genes. We also investigated functional heterogeneity among clinical isolates and whether variation in fimH genes accounts for differences in receptor specificity. Twelve isolates obtained from human urine were tested for their ability to adhere to mannan, fibronectin, periodate-treated fibronectin, and a synthetic peptide copying the 30 amino-terminal residues of fibronectin. CSH-50 and HB101(pPKL4) were tested for comparison. Selected isolates were also tested for adhesion to purified fragments spanning the entire fibronectin molecule. Three distinct functional classes, designated M, MF, and MFP, were observed. The fimH genes were amplified by PCR from chromosomal DNA obtained from representative strains and expressed in a delta fim strain (AAEC191A) transformed with a recombinant plasmid containing the entire fim gene cluster but with a translational stop-linker inserted into the fimH gene (pPKL114). Cloned fimH genes conferred on AAEC191A(pPKL114) receptor specificities mimicking those of the parent strains from which the fimH genes were obtained, demonstrating that the FimH subunits are responsible for the functional heterogeneity. Representative fimH genes were sequenced, and the deduced amino acid sequences were compared with the previously published FimH sequence. Allelic variants exhibiting >98% homology and encoding proteins differing by as little as a single amino acid substitution confer distinct adhesive phenotypes. This unexpected adhesive diversity within the FimH family broadens the scope of potential receptors for enterobacterial adhesion and may lead to a fundamental

  5. Potent In Vitro and In Vivo Activity of Plantibody Specific for Porphyromonas gingivalis FimA.

    PubMed

    Choi, Young-Suk; Moon, Ji-Hoi; Kim, Tae-Geum; Lee, Jin-Yong

    2016-04-01

    Fimbrial protein fimbrillin (FimA), a major structural subunit of Porphyromonas gingivalis, has been suggested as a vaccine candidate to control P. gingivalis-induced periodontal disease. Previously, cDNAs encoding IgG monoclonal antibodies (MAbs) against purified FimA from P. gingivalis 2561 have been cloned, and the MAbs have been produced in rice cell suspension. Here we examined the biological activities of the plant-produced MAb specific for FimA (anti-FimA plantibody) of P. gingivalis in vitro and in vivo. The anti-FimA plantibody recognized oligomeric/polymeric forms of native FimA in immunoblot analysis and showed high affinity for native FimA (KD = 0.11 nM). Binding of P. gingivalis (10(8) cells) to 2 mg of saliva-coated hydroxyapatite beads was reduced by 53.8% in the presence of 1 μg/ml plantibody. Anti-FimA plantibody (10 μg/ml) reduced invasion of periodontal ligament cells by P. gingivalis (multiplicity of infection, 100) by 68.3%. Intracellular killing of P. gingivalis opsonized with the anti-FimA plantibody by mouse macrophages was significantly increased (77.1%) compared to killing of bacterial cells with irrelevant IgG (36.7%). In a mouse subcutaneous chamber model, the number of recoverable P. gingivalis cells from the chamber fluid was significantly reduced when the numbers of bacterial cells opsonized with anti-FimA plantibody were compared with the numbers of bacterial cells with irrelevant IgG, 66.7% and 37.1%, respectively. These in vitro and in vivo effects of anti-FimA plantibody were comparable to those of the parental MAb. Further studies with P. gingivalis strains with different types of fimbriae are needed to investigate the usefulness of anti-FimA plantibody for passive immunization to control P. gingivalis-induced periodontal disease. PMID:26865596

  6. LIS Education in Portugal between Academia and Practice

    ERIC Educational Resources Information Center

    Ribeiro, Fernanda

    2008-01-01

    There is a brief exposition, in a historical perspective, about the evolution of the professional education in Portugal, in the area of Library and Information Science, since the creation of the Curso de Bibliotecario-Arquivista, in 1887, until its closure in 1982, when it was replaced by the Curso de Especializacao em Ciencias Documentais (CECD).…

  7. Functional divergence of FimX in PilZ binding and type IV pilus regulation.

    PubMed

    Qi, Yaning; Xu, Linghui; Dong, Xueming; Yau, Yin Hoe; Ho, Chun Loong; Koh, Siew Lee; Shochat, Susana Geifman; Chou, Shan-Ho; Tang, Kai; Liang, Zhao-Xun

    2012-11-01

    Type IV pili (T4P) are polar surface structures that play important roles in bacterial motility, biofilm formation, and pathogenicity. The protein FimX and its orthologs are known to mediate T4P formation in the human pathogen Pseudomonas aeruginosa and some other bacterial species. It was reported recently that FimX(XAC2398) from Xanthomonas axonopodis pv. citri interacts with PilZ(XAC1133) directly through the nonenzymatic EAL domain of FimX(XAC2398). Here we present experimental data to reveal that the strong interaction between FimX(XAC2398) and PilZ(XAC1133) is not conserved in P. aeruginosa and likely other Pseudomonas species. In vitro and in vivo binding experiments showed that the interaction between FimX and PilZ in P. aeruginosa is below the measurable limit. Surface plasmon resonance assays further confirmed that the interaction between the P. aeruginosa proteins is at least more than 3 orders of magnitude weaker than that between the X. axonopodis pv. citri pair. The N-terminal lobe region of FimX(XAC2398) was identified as the binding surface for PilZ(XAC1133) by amide hydrogen-deuterium exchange and site-directed mutagenesis studies. Lack of several key residues in the N-terminal lobe region of the EAL domain of FimX is likely to account for the greatly reduced binding affinity between FimX and PilZ in P. aeruginosa. All together, the results suggest that the interaction between PilZ and FimX in Xanthomonas species is not conserved in P. aeruginosa due to the evolutionary divergence among the FimX orthologs. The precise roles of FimX and PilZ in bacterial motility and T4P biogenesis are likely to vary among bacterial species. PMID:22942245

  8. Catch-bond mechanism of the bacterial adhesin FimH.

    PubMed

    Sauer, Maximilian M; Jakob, Roman P; Eras, Jonathan; Baday, Sefer; Eriş, Deniz; Navarra, Giulio; Bernèche, Simon; Ernst, Beat; Maier, Timm; Glockshuber, Rudi

    2016-01-01

    Ligand-receptor interactions that are reinforced by mechanical stress, so-called catch-bonds, play a major role in cell-cell adhesion. They critically contribute to widespread urinary tract infections by pathogenic Escherichia coli strains. These pathogens attach to host epithelia via the adhesin FimH, a two-domain protein at the tip of type I pili recognizing terminal mannoses on epithelial glycoproteins. Here we establish peptide-complemented FimH as a model system for fimbrial FimH function. We reveal a three-state mechanism of FimH catch-bond formation based on crystal structures of all states, kinetic analysis of ligand interaction and molecular dynamics simulations. In the absence of tensile force, the FimH pilin domain allosterically accelerates spontaneous ligand dissociation from the FimH lectin domain by 100,000-fold, resulting in weak affinity. Separation of the FimH domains under stress abolishes allosteric interplay and increases the affinity of the lectin domain. Cell tracking demonstrates that rapid ligand dissociation from FimH supports motility of piliated E. coli on mannosylated surfaces in the absence of shear force. PMID:26948702

  9. Catch-bond mechanism of the bacterial adhesin FimH

    PubMed Central

    Sauer, Maximilian M.; Jakob, Roman P.; Eras, Jonathan; Baday, Sefer; Eriş, Deniz; Navarra, Giulio; Bernèche, Simon; Ernst, Beat; Maier, Timm; Glockshuber, Rudi

    2016-01-01

    Ligand–receptor interactions that are reinforced by mechanical stress, so-called catch-bonds, play a major role in cell–cell adhesion. They critically contribute to widespread urinary tract infections by pathogenic Escherichia coli strains. These pathogens attach to host epithelia via the adhesin FimH, a two-domain protein at the tip of type I pili recognizing terminal mannoses on epithelial glycoproteins. Here we establish peptide-complemented FimH as a model system for fimbrial FimH function. We reveal a three-state mechanism of FimH catch-bond formation based on crystal structures of all states, kinetic analysis of ligand interaction and molecular dynamics simulations. In the absence of tensile force, the FimH pilin domain allosterically accelerates spontaneous ligand dissociation from the FimH lectin domain by 100,000-fold, resulting in weak affinity. Separation of the FimH domains under stress abolishes allosteric interplay and increases the affinity of the lectin domain. Cell tracking demonstrates that rapid ligand dissociation from FimH supports motility of piliated E. coli on mannosylated surfaces in the absence of shear force. PMID:26948702

  10. Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube

    PubMed Central

    Zhang, Meng; Zhang, Ruihui; Qu, Xiaolu; Huang, Shanjin

    2016-01-01

    The actin cytoskeleton is increasingly recognized as a major regulator of pollen tube growth. Actin filaments have distinct distribution patterns and dynamic properties within different regions of the pollen tube. Apical actin filaments are highly dynamic and crucial for pollen tube growth. However, how apical actin filaments are generated and properly constructed remains an open question. Here we showed that Arabidopsis fimbrin5 (FIM5) decorates filamentous structures throughout the entire tube but is apically concentrated. Apical actin structures are disorganized to different degrees in the pollen tubes of fim5 loss-of-function mutants. Further observations suggest that apical actin structures are not constructed properly because apical actin filaments cannot be maintained at the cortex of fim5 pollen tubes. Actin filaments appeared to be more curved in fim5 pollen tubes and this was confirmed by measurements showing that the convolutedness and the rate of change of convolutedness of actin filaments was significantly increased in fim5 pollen tubes. This suggests that the rigidity of the actin filaments may be compromised in fim5 pollen tubes. Further, the apical cell wall composition is altered, implying that tip-directed vesicle trafficking events are impaired in fim5 pollen tubes. Thus, we found that FIM5 decorates apical actin filaments and regulates their organization in order to drive polarized pollen tube growth. PMID:27117336

  11. Modernising Portugal's Secondary Schools

    ERIC Educational Resources Information Center

    Heitor, Teresa V.

    2008-01-01

    Portugal has a total of 477 public secondary schools. Some date from the end of the 19th century but the majority were built after 1970, reflecting the period of expansion in the school network and the extension of compulsory schooling. The schools are heterogeneous in terms of building types, architectural features and quality. An assessment of…

  12. Does the Functional Assessment Measure (FAM) Extend the Functional Independence Measure (FIM) Instrument: A Rasch Analysis of Stroke Inpatients.

    ERIC Educational Resources Information Center

    Linn, Richard T.; Blair, Richard S.; Granger, Carl V.; Harper, Dan W.; O'Hara, Patricia A.; Maciura, Edith

    1999-01-01

    Studied whether adding Functional Assessment Measure (FAM) (K. Hall, 1992) scores to those from the Functional Independence Measure (FIM) adds to the FIM range. Results from 376 Canadian patients suggest that using the FAM as an adjunct to the FIM reduces test efficiency and adds minimal benefits. (SLD)

  13. Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways

    PubMed Central

    Whitchurch, Cynthia B.; Beatson, Scott A.; Comolli, James C.; Jakobsen, Thania; Sargent, Jennifer L.; Bertrand, Jacob J.; West, Joyce; Klausen, Mikkel; Waite, Leslie L.; Kang, Pil Jung; Tolker-Nielsen, Tim; Mattick, John S.; Engel, Joanne N.

    2005-01-01

    Virulence of Pseudomonas aeruginosa involves the co-ordinate expression of a range of factors including type IV pili (tfp), the type III secretion system (TTSS) and quorum sensing. Tfp are required for twitching motility, efficient biofilm formation, and for adhesion and type III secretion (TTS)-mediated damage to mammalian cells. We describe a novel gene (fimL) that is required for tfp biogenesis and function, for TTS and for normal biofilm development in P. aeruginosa. The predicted product of fimL is homologous to the N-terminal domain of ChpA, except that its putative histidine and threonine phosphotransfer sites have been replaced with glutamine. fimL mutants resemble vfr mutants in many aspects including increased autolysis, reduced levels of surface-assembled tfp and diminished production of type III secreted effectors. Expression of vfr in trans can complement fimL mutants. vfr transcription and production is reduced in fimL mutants whereas cAMP levels are unaffected. Deletion and insertion mutants of fimL frequently revert to wild-type phenotypes suggesting that an extragenic suppressor mutation is able to overcome the loss of fimL. vfr transcription and production, as well as cAMP levels, are elevated in these revertants, while Pseudomonas quinolone signal (PQS) production is reduced. These results suggest that the site(s) of spontaneous mutation is in a gene(s) which lies upstream of vfr transcription, cAMP, production, and PQS synthesis. Our studies indicate that Vfr and FimL are components of intersecting pathways that control twitching motility, TTSS and autolysis in P. aeruginosa. PMID:15720546

  14. Structure-Based Drug Design and Optimization of Mannoside Bacterial FimH Antagonists

    PubMed Central

    Han, Zhenfu; Pinkner, Jerome S.; Ford, Bradley; Obermann, Robert; Nolan, William; Wildman, Scott A.; Hobbs, Doug; Ellenberger, Tom; Cusumano, Corinne K.; Hultgren, Scott J.; Janetka, James W.

    2010-01-01

    FimH-mediated cellular adhesion to mannosylated proteins is critical in the ability of uropathogenic E. coli (UPEC) to colonize and invade the bladder epithelium during urinary tract infection. We describe the discovery and optimization of potent small-molecule FimH bacterial adhesion antagonists based on α-D-mannose 1-position anomeric glycosides using X-ray structure-guided drug design. Optimized biaryl mannosides display low nanomolar binding affinity for FimH in a fluorescence polarization assay and sub micromolar cellular activity in a hemagglutination (HA) functional cell assay of bacterial adhesion. X-ray crystallography demonstrates that the biphenyl moiety makes several key interactions with the outer surface of FimH including π-π interactions with Tyr-48 and an H-bonding electrostatic interaction with the Arg-98/Glu-50 salt-bridge. Dimeric analogs linked through the biaryl ring show an impressive 8-fold increase in potency relative to monomeric matched pairs and represent the most potent FimH antagonists identified to date. The FimH antagonists described herein hold great potential for development as novel therapeutics for the effective treatment of urinary tract infections. PMID:20507142

  15. Phytoplankton Bloom Off Portugal

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Turquoise and greenish swirls marked the presence of a large phytoplankton bloom off the coast of Portugal on April 23, 2002. This true-color image was acquired by the Moderate-resolution Imaging Spectroradiometer (MODIS), flying aboard NASA's Terra satellite. There are also several fires burning in northwest Spain, near the port city of A Coruna. Please note that the high-resolution scene provided here is 500 meters per pixel. For a copy of this scene at the sensor's fullest resolution, visit the MODIS Rapidfire site.

  16. Detection of antibodies against fimbria type 3 (Fim3) is useful diagnostic assay for pertussis.

    PubMed

    Oguchi, Kaoru; Miyata, Akiko; Kazuyama, Yukimasa; Noda, Atsuya; Suzuki, Eri; Watanabe, Mineo; Nakayama, Tetsuo

    2015-09-01

    Isolation of Bordetella pertussis and detection of the pertussis genome are not always successful because of low bacterial loads in adult patients with pertussis. Antibodies against pertussis toxin (PT) are measured but have low sensitivity in vaccinated subjects. There is no reliable diagnostic method at present. In this study, a fluorescent-EIA against several pertussis antigens and genome detection were investigated to establish clinical laboratory diagnostic methods for pertussis. The study was conducted in an outpatient clinic between September 2007 and 2013. Subjects consisted of 209 patients including adults suspected of pertussis and 35 staff members of the clinic. Loop-mediated isothermal amplification (LAMP) was performed to detect the pertussis genome in 5' UTR of the pertussis toxin (PT) gene. The catalytic region of the adenylate cyclase toxin (catACT), C-terminal of filamentous hemagglutinin (cFHA), and type 3 fimbria (Fim3) were selected, which are not pertussis vaccine component. Conventional PT and FHA antibodies were examined together with type 2 fimbria (Fim2) antibodies, and these are vaccine antigens. Pertussis DNA was detected in 23 (11%) out of 209. Detection sensitivity was high in young infants. Antibodies against Fim3 showed a higher positive rate in all age groups. Staff members at the pediatric outpatient clinic showed serological booster responses in Fim2 and Fim3 antibodies more sensitively than those in PT antibodies during outbreaks. LAMP was useful for detecting the pertussis genome in young infants, whereas a serological assay for fluorescent-EIA against Fim2 and Fim3 was preferable for adolescents and adults. PMID:26134278

  17. Role of the Bordetella pertussis minor fimbrial subunit, FimD, in colonization of the mouse respiratory tract.

    PubMed Central

    Geuijen, C A; Willems, R J; Bongaerts, M; Top, J; Gielen, H; Mooi, F R

    1997-01-01

    Bordetella pertussis fimbriae are composed of a major subunit, Fim2 or Fim3, and the minor subunit FimD. Using immunoelectron microscopy, we provide evidence that FimD is located at the fimbrial tip. The role of FimD in colonization of the mouse respiratory tract was studied by using two fimbrial mutants: a mutant completely devoid of fimbriae (designated FimD-) and a mutant devoid of the major fimbrial subunits but still producing the minor subunit (designated FimD+). The ability of the two fimbrial mutants to colonize the nasopharynx, trachea, and lungs was compared with those of the wild type parental strain and a filamentous hemagglutinin (FHA) mutant. Of the three mutants studied, the FimD- mutant showed the greatest defect, colonizing less well in the nasopharynx, trachea, and lungs. The most pronounced defect in colonizing ability of the three mutants was observed in the trachea. However, the colonizing defect of the FHA and FimD+ mutants in the trachea was observed only during the first 3 days of infection. After 10 days, the colonization level was nearly restored to wild-type levels. The FHA and FimD+ mutants showed a slight colonization defect in the nasopharynx but no defect in the lungs. A maltose binding protein-FimD fusion protein and a peptide derived from FimD were able to bind to heparin, a member of a class of sulfated sugars which are ubiquitous in the respiratory tract. Recently it was shown (W. L. W. Hazenbos, C. A. W. Geuijen, B. M. van den Berg, F. R. Mooi, and R. van Furth, J. Infect. Dis. 171:924-929, 1995) that FimD also binds to the integrin VLA-5, and our results suggest that the binding of B. pertussis to these two molecules plays an important role in colonization of the respiratory tract of the mouse. PMID:9317030

  18. Oral Immunization with Recombinant Streptococcus gordonii Expressing Porphyromonas gingivalis FimA Domains

    PubMed Central

    Sharma, Ashu; Honma, Kiyonobu; Evans, Richard T.; Hruby, Dennis E.; Genco, Robert J.

    2001-01-01

    Porphyromonas gingivalis, a gram-negative anaerobe, is implicated in the etiology of adult periodontitis. P. gingivalis fimbriae are one of several critical surface virulence factors involved in both bacterial adherence and inflammation. P. gingivalis fimbrillin (FimA), the major subunit protein of fimbriae, is considered an important antigen for vaccine development against P. gingivalis-associated periodontitis. We have previously shown that biologically active domains of P. gingivalis fimbrillin can be expressed on the surface of the human commensal bacterium Streptococcus gordonii. In this study, we examined the effects of oral coimmunization of germfree rats with two S. gordonii recombinants expressing N (residues 55 to 145)- and C (residues 226 to 337)-terminal epitopes of P. gingivalis FimA to elicit FimA-specific immune responses. The effectiveness of immunization in protecting against alveolar bone loss following P. gingivalis infection was also evaluated. The results of this study show that the oral delivery of P. gingivalis FimA epitopes via S. gordonii vectors resulted in the induction of FimA-specific serum (immunoglobulin G [IgG] and IgA) and salivary (IgA) antibody responses and that the immune responses were protective against subsequent P. gingivalis-induced alveolar bone loss. These results support the potential usefulness of the S. gordonii vectors expressing P. gingivalis fimbrillin as a mucosal vaccine against adult periodontitis. PMID:11292708

  19. Early Childhood Education in Portugal.

    ERIC Educational Resources Information Center

    Vasconcelos, Teresa

    This booklet provides an overview of the policy and status of early childhood education in Portugal and includes the text of Law 5/97, the Framework Law for Pre-School Education, as well as the Curriculum Guidelines for Pre-School Education adopted by Portugal's government. Two of Law 5/97's nine main goals are: (1) to promote the child's personal…

  20. Aerosol impact on seasonal prediction using FIM-Chem-iHYCOM coupled model

    NASA Astrophysics Data System (ADS)

    sun, shan; Grell, Georg; Bleck, Rainer

    2016-04-01

    A coupled model consisting of the weather model FIM and the ocean model iHYCOM, both operating on an icosahedral horizontal grid, is being developed for subseasonal to seasonal prediction. Initial results indicate that the model skill is comparable to that of the operational model CFSv2 used by NCEP. In addition, an online atmospheric chemistry module is coupled to FIM. The purpose of onging experiments with the FIM-Chem-iHYCOM combination is to investigate the aerosol impact on the atmospheric and oceanic circulation at the seasonal scale. We compare the model sensitivity with various chemistry emissions, including aerosols, fire and anthropogenic emissions. Additional emphasis of this work is on the effect of aerosols on cloudiness and precipitation, either directly or indirectly through changes in SST. To isolate the latter effect, we conduct parallel experiments with observed SST.

  1. Women in Portugal.

    PubMed

    Barbosa, M

    1981-01-01

    Prior to 1974, women in Portugal were restricted by the Penal Code and had little organizational power. Women's groups were formed within the Catholic Church to teach women about cooking, child care, and home economics. There was no contact with international women's groups. The press only reported events such as bra burning. 80% of all illiterates in Portugal are women. The conditions of Portuguese women are described after the revolution of April 25, 1974. Present roles are discussed for work, health, education, religion, trade unions and political parties, and women's organizations. The Women's Liberation Movement (WLM) appeared in May 1974 among a heterogenous group of women in Lisbon who were concerned about the oppression of women. WLM made feminist issues public amid ridicule and promoted the declaration of equal rights for women in the 1976 Republic Constitution and in the Family Code. Wage discrimination became illegal in 1979. Women represent 32.8% of the labor force. Unemployment is particularly high among women and is increasing. Women's wages and levels of skill are the lowest. The Christian Democratic government is actively engaged in a campaign to keep women at home and has formed the special Ministry of Family Affairs, which encourages large families and women's home activity in order to save jobs for men. There is a crisis in education: large class sizes and limited number of schools. Child care for the working mother is expensive when available and rarely available. An obstacle to women's rights has been the role of the Catholic Church, which fought equal rights legislation, condemned the Family Code and divorce laws, forbade the practice of contraception, and supported the movement against abortion. Only 1 member of government is a women, and she is considered a token. Trade unions have a women's section, but little attention is given to the problems of women. Women's groups within larger organizations have little autonomy. Those with autonomy

  2. Differentiating organic from conventional peppermints using chromatographic and flow-injection mass spectrometric (FIMS) fingerprints

    Technology Transfer Automated Retrieval System (TEKTRAN)

    High performance liquid chromatography (HPLC) and flow-injection mass spectrometric (FIMS) fingerprinting techniques were tested for their potential in differentiating organic and conventional peppermint samples. Ten organic and ten conventional peppermint samples were examined using HPLC-UV and FI...

  3. FIM, a Novel FTIR-Based Imaging Method for High Throughput Locomotion Analysis

    PubMed Central

    Otto, Nils; Löpmeier, Tim; Valkov, Dimitar; Jiang, Xiaoyi; Klämbt, Christian

    2013-01-01

    We designed a novel imaging technique based on frustrated total internal reflection (FTIR) to obtain high resolution and high contrast movies. This FTIR-based Imaging Method (FIM) is suitable for a wide range of biological applications and a wide range of organisms. It operates at all wavelengths permitting the in vivo detection of fluorescent proteins. To demonstrate the benefits of FIM, we analyzed large groups of crawling Drosophila larvae. The number of analyzable locomotion tracks was increased by implementing a new software module capable of preserving larval identity during most collision events. This module is integrated in our new tracking program named FIMTrack which subsequently extracts a number of features required for the analysis of complex locomotion phenotypes. FIM enables high throughput screening for even subtle behavioral phenotypes. We tested this newly developed setup by analyzing locomotion deficits caused by the glial knockdown of several genes. Suppression of kinesin heavy chain (khc) or rab30 function led to contraction pattern or head sweeping defects, which escaped in previous analysis. Thus, FIM permits forward genetic screens aimed to unravel the neural basis of behavior. PMID:23349775

  4. Focused Impedance Method (FIM) and Pigeon Hole Imaging (PHI) for localized measurements - a review

    NASA Astrophysics Data System (ADS)

    Siddique-e Rabbani, K.

    2010-04-01

    This paper summarises up to date development in Focused Impedance Method (FIM) initiated by us. It basically involves taking the sum of two orthogonal tetra-polar impedance measurements around a common central region, giving a localized enhanced sensitivity. Although the basic idea requires 8 electrodes, versions with 6- and 4-electrodes were subsequently conceived and developed. The focusing effect has been verified in 2D and 3D phantoms and through numerical analysis. Dynamic stomach emptying, and ventilation of localized lung regions have been studied successfully suggesting further applications in monitoring of gastric acid secretion, artificial respiration, bladder emptying, etc. Multi-frequency FIM may help identify some diseases and disorders including certain cancers. FIM, being much simpler and having less number of electrodes, appears to have the potential to replace EIT for applications involving large and shallow organs. An enhancement of 6-electrode FIM led to Pigeon Hole Imaging (PHI) in a square matrix through backprojection in two orthogonal directions, good for localising of one or two well separated objects.

  5. RfaH Suppresses Small RNA MicA Inhibition of fimB Expression in Escherichia coli K-12

    PubMed Central

    Moores, Alexandra; Chipper-Keating, Saranna; Sun, Lei; McVicker, Gareth; Wales, Lynn; Gashi, Krishna

    2014-01-01

    The phase variation (reversible on-off switching) of the type 1 fimbrial adhesin of Escherichia coli involves a DNA inversion catalyzed by FimB (switching in either direction) or FimE (on-to-off switching). Here, we demonstrate that RfaH activates expression of a FimB-LacZ protein fusion while having a modest inhibitory effect on a comparable fimB-lacZ operon construct and on a FimE-LacZ protein fusion, indicating that RfaH selectively controls fimB expression at the posttranscriptional level. Further work demonstrates that loss of RfaH enables small RNA (sRNA) MicA inhibition of fimB expression even in the absence of exogenous inducing stress. This effect is explained by induction of σE, and hence MicA, in the absence of RfaH. Additional work confirms that the procaine-dependent induction of micA requires OmpR, as reported previously (A. Coornaert et al., Mol. Microbiol. 76:467–479, 2010, doi:10.1111/j.1365-2958.2010.07115.x), but also demonstrates that RfaH inhibition of fimB transcription is enhanced by procaine independently of OmpR. While the effect of procaine on fimB transcription is shown to be independent of RcsB, it was found to require SlyA, another known regulator of fimB transcription. These results demonstrate a complex role for RfaH as a regulator of fimB expression. PMID:24163336

  6. FimH adhesin from host unrestricted Salmonella Enteritidis binds to different glycoprotein ligands expressed by enterocytes from sheep, pig and cattle than FimH adhesins from host restricted Salmonella Abortus-ovis, Salmonella Choleraesuis and Salmonella Dublin.

    PubMed

    Grzymajło, Krzysztof; Ugorski, Maciej; Kolenda, Rafał; Kędzierska, Anna; Kuźmińska-Bajor, Marta; Wieliczko, Alina

    2013-10-25

    Adhesion to gut tissues and colonization of the alimentary tract, two important stages in the pathogenesis of Salmonella, are mediated by FimH adhesin of type 1 fimbriae. It was suggested that minor differences in the structure of FimH are most likely associated with differences in adhesion specificities, and may determine the tropism of various Salmonella serovars to different species and tissues. We investigated this hypothesis by comparing the binding properties of FimH proteins from three Salmonella enterica serovars with limited (Choleraesuis, Dublin) or restricted (Abortusovis) host ranges to FimH from broad host range S. Enteritidis and mannose inactive FimH from S. Gallinarum. Although all active variants of FimH protein were able to bind mannose-rich glycoproteins (RNase B, HRP and Man-BSA) with comparable affinity measured by surface plasmon resonance, there were significant differences in the binding profiles of the FimH proteins from host restricted serovars and host unrestricted serovar Enteritidis, to glycoproteins from enterocyte cell lines established in vitro and derived from sheep, pig and cattle. When low-binding FimH adhesin from S. Enteritidis was subjected to Western blot analysis, it bound to surface membrane protein of about 130 kDa, and high-binding FimH adhesins from S. Abortusovis, S. Choleraesuis and S. Dublin bound to surface membrane protein of about 55 kDa present in each cell line. Differential binding of FimH proteins from host-restricted and broad-host-range Salmonella to intestinal receptors was confirmed using mutant FimH adhesins obtained by site-directed mutagenesis. It was found that the low-binding variant of FimH from S. Choleraesuis with mutation Leu57Pro lost the ability to bind protein band of 55 kDa, but instead interacted with glycoprotein of about 130 kDa. On the other hand, the high-binding variant of FimH adhesin from S. Enteritids with mutation Asn101Ser did not bind to its receptor of 130 kDa, but instead it

  7. Textiles and Training in Portugal.

    ERIC Educational Resources Information Center

    Andrez, Jaime Serrao; Dias, Mario Caldeira

    Analyzing the role of vocational training in an economic sector that is declining in Portugal, this document consists of five chapters, a bibliography, and a list of training organizations. An introduction tells why the study is important and explains that the major obstacles to development of the Portuguese textile and clothing sector are the…

  8. Portugal's Secondary School Modernisation Programme

    ERIC Educational Resources Information Center

    Heitor, Teresa V.; Freire da Silva, Jose M. R.

    2009-01-01

    The aim of the Secondary School Modernisation Programme, being implemented in Portugal by "Parque Escolar, EPE", is based on the pursuit of quality and makes Portuguese education a potential international benchmark. This paper discusses the strategies adopted to reorganise school spaces. It describes the conceptual model and highlights the…

  9. Second generation of thiazolylmannosides, FimH antagonists for E. coli-induced Crohn's disease.

    PubMed

    Chalopin, T; Alvarez Dorta, D; Sivignon, A; Caudan, M; Dumych, T I; Bilyy, R O; Deniaud, D; Barnich, N; Bouckaert, J; Gouin, S G

    2016-04-28

    The anti-adhesive strategy, consisting of disrupting bacterial attachment to the host cells, is widely explored as an alternative to antibiotic therapies. Recently, thiazolylmannosides (TazMans) have been identified as strong anti-adhesives of E. coli strains implied in the gut inflammation of patients with Crohn's disease. In this work, we developed a second generation of TazMans with improved chemical stability. The anomeric nitrogen was substituted by short linkers and the compounds were assessed against the bacterial adhesin FimH and the clinically isolated LF82 E. coli strain in four in vitro assays. The results obtained on the FimH adhesin alone and the whole bacteria enabled the identification of a candidate for further in vivo evaluations. PMID:27043998

  10. Sex education in Portugal.

    PubMed

    Frade, A; Vilar, D

    1991-05-01

    The article on sex education in Portugal covers background, the educational system, the clashes of the 1960's over sex education, the Committee for the Study of Sexuality and Education (CSSE), the policies, politics and social movements during the period 1974 - 1984, the discussions in Parliament, the 1988 Reform of the Educational System, the Family Planning Association (FPA) and sex education, and the future role of the FPA. It was not until the institution of the multiparity parliamentary system in 1974 that discussing social and political changes was possible, culminating in 1984 with new legislation on abortion, family planning, and sex education. School reform came in 1987/8 with the Ministry of Education primarily responsible for curricula. The 1960's brought with it the influence of the Catholic Church. Change came in the form of progressivism among Catholics who replaced dogma with dialogue and listening. Sex education was considered as preparation for marriage, but masturbation, contraception, and prostitution were also discussed. In addition, the founder of FPA chaired the CSSE in 1971 and opened up debate on sex issues and drafted a bill to establish co-education in Portuguese schools. The revolution of 1974 brought an end to censorship and brought forth a policy of developing family planning. Changed in the Family Code gave women greater equality. UNFPA supported teacher training in non-sexist education. With human reproduction included in the natural sciences, there was still no school sex education policy and contraception was only sometimes represented in the biology curriculum. The focus of FPA was on contraception and abortion. Finally in the 1980's, the first sex education programs were developed for out-of-school youth. Even though in the 1970's there were leftists groups promoting sex education, it took leftist parliamentary power to get legislation on sex education in the schools adopted. The Ministry of Education however was pressured by the

  11. FIM FRAME: a method for assessing and improving emergency plans for floods

    NASA Astrophysics Data System (ADS)

    Lumbroso, D. M.; Di Mauro, M.; Tagg, A. F.; Vinet, F.; Stone, K.

    2012-05-01

    Over the past decade Europe has been hit by a number of severe flood events. Reviews of recent large flood events in England and France have indicated that there is room for improvement in the emergency planning for floods. Methods that can be used for the systematic assessment and improvement of emergency plans are extensively documented in readily available literature. However, those that do exist are often limited to appraising the content of the plans rather than the process that the plan should guide. This paper describes research to develop a systematic method for assessing and improving emergency plans, which is called the FIM FRAME method. The development of the method was informed by research carried out with stakeholders in France, the Netherlands and England, as well as an appraisal of available tools that can be used to develop and improve plans, and an analysis of a selection of flood emergency plans from the three countries. One of the fundamental requirements of the FIM FRAME method was that it should be able to be applied by the relevant stakeholders to a range of emergency plans that mainly focus on flooding. The method comprises a series of steps (known as Appraise, Tackle and Implement) that can assist stakeholders with assessing and improving emergency plans. The method was piloted in the three countries and then refined following feedback from end users. This paper describes the development of the FIM FRAME method and its application in three case studies affected by different types of floods.

  12. Quantitative differences in adhesiveness of type 1 fimbriated Escherichia coli due to structural differences in fimH genes.

    PubMed Central

    Sokurenko, E V; Courtney, H S; Maslow, J; Siitonen, A; Hasty, D L

    1995-01-01

    Type 1 fimbriae are heteropolymeric surface organelles responsible for the D-mannose-sensitive (MS) adhesion of Escherichia coli. We recently reported that variation of receptor specificity of type 1 fimbriae can result solely from minor alterations in the structure of the gene for the FimH adhesin subunit. To further study the relationship between allelic variation of the fimH gene and adhesive properties of type 1 fimbriae, the fimH genes from five additional strains were cloned and used to complement the FimH deletion in E. coli KB18. When the parental and recombinant strains were tested for adhesion to immobilized mannan, a wide quantitative range in the ability of bacteria to adhere was noted. The differences in adhesion do not appear to be due to differences in the levels of fimbriation or relative levels of incorporation of FimH, because these parameters were similar in low-adhesion and high-adhesion strains. The nucleotide sequence for each of the fimH genes was determined. Analysis of deduced FimH sequences allowed identification of two sequence homology groups, based on the presence of Asn-70 and Ser-78 or Ser-70 and Asn-78 residues. The consensus sequences for each group conferred very low adhesion activity, and this low-adhesion phenotype predominated among a group of 43 fecal isolates. Strains isolated from a different host niche, the urinary tract, expressed type 1 fimbriae that conferred an increased level of adhesion. The results presented here strongly suggest that the quantitative variations in MS adhesion are due primarily to structural differences in the FimH adhesin. The observed differences in MS adhesion among populations of E. coli isolated from different host niches call attention to the possibility that phenotypic variants of FimH may play a functional role in populations dynamics. PMID:7601831

  13. Aircraft Configuration and Flight Crew Compliance with Procedures While Conducting Flight Deck Based Interval Management (FIM) Operations

    NASA Technical Reports Server (NTRS)

    Shay, Rick; Swieringa, Kurt A.; Baxley, Brian T.

    2012-01-01

    Flight deck based Interval Management (FIM) applications using ADS-B are being developed to improve both the safety and capacity of the National Airspace System (NAS). FIM is expected to improve the safety and efficiency of the NAS by giving pilots the technology and procedures to precisely achieve an interval behind the preceding aircraft by a specific point. Concurrently but independently, Optimized Profile Descents (OPD) are being developed to help reduce fuel consumption and noise, however, the range of speeds available when flying an OPD results in a decrease in the delivery precision of aircraft to the runway. This requires the addition of a spacing buffer between aircraft, reducing system throughput. FIM addresses this problem by providing pilots with speed guidance to achieve a precise interval behind another aircraft, even while flying optimized descents. The Interval Management with Spacing to Parallel Dependent Runways (IMSPiDR) human-in-the-loop experiment employed 24 commercial pilots to explore the use of FIM equipment to conduct spacing operations behind two aircraft arriving to parallel runways, while flying an OPD during high-density operations. This paper describes the impact of variations in pilot operations; in particular configuring the aircraft, their compliance with FIM operating procedures, and their response to changes of the FIM speed. An example of the displayed FIM speeds used incorrectly by a pilot is also discussed. Finally, this paper examines the relationship between achieving airline operational goals for individual aircraft and the need for ATC to deliver aircraft to the runway with greater precision. The results show that aircraft can fly an OPD and conduct FIM operations to dependent parallel runways, enabling operational goals to be achieved efficiently while maintaining system throughput.

  14. [Demography and employment in Portugal].

    PubMed

    Barata, O S

    1981-01-01

    The population of Portugal showed a period of slow growth between 1950-60; however, in the 1970s, the return of large numbers of former residents in African territories along with a reduction in emigration changed the trend so that the 1981 census showed a significant increase. The Portuguese economy, on the other hand, is in a state of crisis which has resulted in large numbers of unemployed. The growth of the population along with these hard times has made it more difficult to reduce the rate of unemployment. It is also more difficult for those leaving school to find jobs. The better educated generations are seeking jobs in industry and in other services that Portugal will find difficult to offer in sufficient numbers in the immediate future. At present, the Portuguese economy has a large component of agricultural labor. In any case, the means of economic and social intervention to fight unemployment have limited potential. Therefore, many of those unable to find jobs in Portugal will attempt to emigrate. Many Portuguese are already working in Germany, France, and in other Western European countries but migration today is much more difficult. In addition, these countries cannot be expected to recive many more migrant workers in the future. In fact, those better educated workers from Portugal will not be very interested in the low paying jobs which can be found more easily by foreign workers in Western Euorpe. Many will therefore attempt to find jobs in non-European countries. There has been a recent increase of migration to Canada and the US. A renewal of interest in jobs in Brazil and other South Amerian countries is also to be expected. There may also be a future increase in the number of experts, technicians, and other qualified personnel emigrating to Portuguese speaking African countries if there is adequate security and if these countries find the way to expand economic growth. (author's modified) PMID:12339773

  15. Structures of C-mannosylated anti-adhesives bound to the type 1 fimbrial FimH adhesin.

    PubMed

    de Ruyck, Jerome; Lensink, Marc F; Bouckaert, Julie

    2016-05-01

    Selective inhibitors of the type 1 fimbrial adhesin FimH are recognized as attractive alternatives for antibiotic therapies and prophylaxes against Escherichia coli infections such as urinary-tract infections. To construct these inhibitors, the α-d-mannopyranoside of high-mannose N-glycans, recognized with exclusive specificity on glycoprotein receptors by FimH, forms the basal structure. A hydrophobic aglycon is then linked to the mannose by the O1 oxygen inherently present in the α-anomeric configuration. Substitution of this O atom by a carbon introduces a C-glycosidic bond, which may enhance the therapeutic potential of such compounds owing to the inability of enzymes to degrade C-glycosidic bonds. Here, the first crystal structures of the E. coli FimH adhesin in complex with C-glycosidically linked mannopyranosides are presented. These findings explain the role of the spacer in positioning biphenyl ligands for interactions by means of aromatic stacking in the tyrosine gate of FimH and how the normally hydrated C-glycosidic link is tolerated. As these new compounds can bind FimH, it can be assumed that they have the potential to serve as potent new antagonists of FimH, paving the way for the design of a new family of anti-adhesive compounds against urinary-tract infections. PMID:27158502

  16. Structures of C-mannosylated anti-adhesives bound to the type 1 fimbrial FimH adhesin

    PubMed Central

    de Ruyck, Jerome; Lensink, Marc F.; Bouckaert, Julie

    2016-01-01

    Selective inhibitors of the type 1 fimbrial adhesin FimH are recognized as attractive alternatives for antibiotic therapies and prophylaxes against Escherichia coli infections such as urinary-tract infections. To construct these inhibitors, the α-d-mannopyranoside of high-mannose N-glycans, recognized with exclusive specificity on glycoprotein receptors by FimH, forms the basal structure. A hydrophobic aglycon is then linked to the mannose by the O1 oxygen inherently present in the α-anomeric configuration. Substitution of this O atom by a carbon introduces a C-glycosidic bond, which may enhance the therapeutic potential of such compounds owing to the inability of enzymes to degrade C-glycosidic bonds. Here, the first crystal structures of the E. coli FimH adhesin in complex with C-glycosidically linked mannopyranosides are presented. These findings explain the role of the spacer in positioning biphenyl ligands for interactions by means of aromatic stacking in the tyrosine gate of FimH and how the normally hydrated C-glycosidic link is tolerated. As these new compounds can bind FimH, it can be assumed that they have the potential to serve as potent new antagonists of FimH, paving the way for the design of a new family of anti-adhesive compounds against urinary-tract infections. PMID:27158502

  17. 2015-16 results from the FIM global model for medium-range to subseasonal forecast duration

    NASA Astrophysics Data System (ADS)

    Benjamin, Stan; Sun, Shan; Bleck, Rainer; Grell, Georg; Bao, Jian-Wen; Brown, John

    2016-04-01

    NOAA/ESRL has continued to develop numerical, physics, and coupled ocean refinements to the FIM global model (Flow-following finite-volume) Icosahedral Model) with a unique combination of an adaptive largely isentropic vertical grid and an icosahedral horizontal grid. The FIM model is being applied to both medium-range and seasonal prediction with considerable success and is a candidate for NOAA week 3-4 prediction within a multi-model ensemble and as a research earth system simulator with ocean and inline chemistry components already in use. FIM is currently run at down to 10-km resolution in real-time. Design improvements from FIM will be applied, in part, toward NOAA's future Next-Generation Global Prediction System (NGGPS). 2015-2016 performance of the FIM global model vs. GFS and other models for medium-range prediction will be summarized. In 2015 controlled experiments, 30km FIM shows improved anomaly correlation attributable to its dynamic core. The presentation will include detail on its coupled with inline chemistry and a matching icosahedral version of the HYCOM ocean model.

  18. Comprehensive analysis of type 1 fimbriae regulation in fimB-null strains from the multidrug resistant Escherichia coli ST131 clone.

    PubMed

    Sarkar, Sohinee; Roberts, Leah W; Phan, Minh-Duy; Tan, Lendl; Lo, Alvin W; Peters, Kate M; Paterson, David L; Upton, Mathew; Ulett, Glen C; Beatson, Scott A; Totsika, Makrina; Schembri, Mark A

    2016-09-01

    Uropathogenic Escherichia coli (UPEC) of sequence type 131 (ST131) are a pandemic multidrug resistant clone associated with urinary tract and bloodstream infections. Type 1 fimbriae, a major UPEC virulence factor, are essential for ST131 bladder colonization. The globally dominant sub-lineage of ST131 strains, clade C/H30-R, possess an ISEc55 insertion in the fimB gene that controls phase-variable type 1 fimbriae expression via the invertible fimS promoter. We report that inactivation of fimB in these strains causes altered regulation of type 1 fimbriae expression. Using a novel read-mapping approach based on Illumina sequencing, we demonstrate that 'off' to 'on' fimS inversion is reduced in these strains and controlled by recombinases encoded by the fimE and fimX genes. Unlike typical UPEC strains, the nucleoid-associated H-NS protein does not strongly repress fimE transcription in clade C ST131 strains. Using a genetic screen to identify novel regulators of fimE and fimX in the clade C ST131 strain EC958, we defined a new role for the guaB gene in the regulation of type 1 fimbriae and in colonisation of the mouse bladder. Our results provide a comprehensive analysis of type 1 fimbriae regulation in ST131, and highlight important differences in its control compared to non-ST131 UPEC. PMID:27309594

  19. Are D-manno-configured Amadori products ligands of the bacterial lectin FimH?

    PubMed Central

    2015-01-01

    Summary The Amadori rearrangement was employed for the synthesis of C-glycosyl-type D-mannoside analogues, namely 1-propargylamino- and 1-phenylamino-1-deoxy-α-D-manno-heptopyranose. They were investigated as ligands of type 1-fimbriated E. coli bacteria by means of molecular docking and bacterial adhesion studies. It turns out that Amadori rearrangement products have a limited activity as inhibitors of bacterial adhesion because the β-C-glycosidically linked aglycone considerably hampers complexation within the carbohydrate binding site of the type 1-fimbrial lectin FimH. PMID:26199665

  20. Are D-manno-configured Amadori products ligands of the bacterial lectin FimH?

    PubMed

    Gloe, Tobias-Elias; Stamer, Insa; Hojnik, Cornelia; Wrodnigg, Tanja M; Lindhorst, Thisbe K

    2015-01-01

    The Amadori rearrangement was employed for the synthesis of C-glycosyl-type D-mannoside analogues, namely 1-propargylamino- and 1-phenylamino-1-deoxy-α-D-manno-heptopyranose. They were investigated as ligands of type 1-fimbriated E. coli bacteria by means of molecular docking and bacterial adhesion studies. It turns out that Amadori rearrangement products have a limited activity as inhibitors of bacterial adhesion because the β-C-glycosidically linked aglycone considerably hampers complexation within the carbohydrate binding site of the type 1-fimbrial lectin FimH. PMID:26199665

  1. Fim study on the relaxation and crystallization processes of a Cu-Zr amorphous alloy

    NASA Astrophysics Data System (ADS)

    H, Lu; Lu, Hua; D, S. Tang; Tang, Disheng; Y, Y. Xiong; Xiong, Yanyun

    1987-09-01

    The relaxation of the Cu-50at.%Zr amorphous alloy was revealed by FIM as a process of formation of clusters consisting of 2, 3, or 4 atoms, which afterwards migrate towards some definite centers, predominantly the quenched-in "embryos", to construct ordered structure. This dynamic picture, so far as we know, is observed for the first time. Crystallized regions were determined by atom-probe analysis as Cu10Zr7 phase, and the coexisting phase CuZr2 was not revealed simultaneously.

  2. Child Labour and Educational Success in Portugal

    ERIC Educational Resources Information Center

    Goulart, Pedro; Bedi, Arjun S.

    2008-01-01

    The current debate on child labour focuses on developing countries. However, Portugal is an example of a relatively developed country where child labour is still a matter of concern as between 8% and 12% of Portuguese children may be classified as workers. This paper studies the patterns of child labour in Portugal and assesses the consequences of…

  3. Portugal to Accede to ESO

    NASA Astrophysics Data System (ADS)

    2000-06-01

    The Republic of Portugal will become the ninth member state of the European Southern Observatory (ESO) [1]. Today, during a ceremony at the ESO Headquarters in Garching (Germany), a corresponding Agreement was signed by the Portuguese Minister of Science and Technology, José Mariano Gago and the ESO Director General, Catherine Cesarsky , in the presence of other high officials from Portugal and the ESO member states (see Video Clip 05/00 below). Following subsequent ratification by the Portuguese Parliament of the ESO Convention and the associated protocols [2], it is foreseen that Portugal will formally join this organisation on January 1, 2001. Uniting European Astronomy ESO PR Photo 16/00 ESO PR Photo 16/00 [Preview - JPEG: 400 x 405 pix - 160k] [Normal - JPEG: 800 x 809 pix - 408k] Caption : Signing of the Portugal-ESO Agreement on June 27, 2000, at the ESO Headquarters in Garching (Germany). At the table, the ESO Director General, Catherine Cesarsky , and the Portuguese Minister of Science and Technology, José Mariano Gago . In his speech, the Portuguese Minister of Science and Technology, José Mariano Gago , stated that "the accession of Portugal to ESO is the result of a joint effort by ESO and Portugal during the last ten years. It was made possible by the rapid Portuguese scientific development and by the growth and internationalisation of its scientific community." He continued: "Portugal is fully committed to European scientific and technological development. We will devote our best efforts to the success of ESO". Catherine Cesarsky , ESO Director General since 1999, warmly welcomed the Portuguese intention to join ESO. "With the accession of their country to ESO, Portuguese astronomers will have great opportunities for working on research programmes at the frontiers of modern astrophysics." "This is indeed a good time to join ESO", she added. "The four 8.2-m VLT Unit Telescopes with their many first-class instruments are nearly ready, and the VLT

  4. ADL structure for nondisabled Japanese children based on the Functional Independence Measure for Children (WeeFIM).

    PubMed

    Tsuji, T; Liu, M; Toikawa, H; Hanayama, K; Sonoda, S; Chino, N

    1999-01-01

    The Functional Independence Measure for Children (WeeFIM) instrument was developed, based on the FIM instrument, to assess disability in children aged 6 mo to 7 yr. Normative data are reported for American and Japanese children, and it is increasingly used for the disabled. Our purpose was to confirm scale quality and to determine the difficulty pattern of the WeeFIM in Japan. The WeeFIM was measured in 225 children (113 girls and 112 boys) aged 6 mo to 7 yr without developmental delays. The scores were converted to an interval scale by Rasch analysis, which assumes unidimensionality of the measurement items, determines the degree of the fit to the assumption, and decides item difficulty. When the WeeFIM items were divided into two groups of motor and cognitive items to minimize misfit, the degree of misfit was acceptable, except for eating, bladder management, tub/shower transfer, and comprehension. For the motor items, grooming, bathing, and bladder control were more difficult, and stairs, bed/chair transfer, and walk or wheelchair were easier. Concerning the cognitive items, expression and comprehension were easier, and problem-solving was most difficult. When we compared item difficulty patterns in the four age groups (6-21 mo, 22-45 mo, 46-62 mo, and 63-100 mo), we found no differences, except in toilet transfer. It was more difficult for younger children, possibly because of its different pattern of chronological change, which shows rapid changes from dependent to independent levels over a short period of time. Our study confirmed the scale quality of the WeeFIM instrument with Rasch analysis and demonstrated the difficulty pattern of the WeeFIM in nondisabled Japanese children. PMID:10340416

  5. Geology and religion in Portugal

    PubMed Central

    Carneiro, Ana; Simoes, Ana; Diogo, Maria Paula; Mota, Teresa Salomé

    2013-01-01

    This paper addresses the relationship between geology and religion in Portugal by focusing on three case studies of naturalists who produced original research and lived in different historical periods, from the eighteenth to the twentieth century. Whereas in non-peripheral European countries religious themes and even controversies between science and religion were dealt with by scientists and discussed in scientific communities, in Portugal the absence of a debate between science and religion within scientific and intellectual circles is particularly striking. From the historiographic point of view, in a country such as Portugal, where Roman Catholicism is part of the religious and cultural tradition, the influence of religion in all aspects of life has been either taken for granted by those less familiar with the national context or dismissed by local intellectuals, who do not see it as relevant to science. The situation is more complex than these dichotomies, rendering the study of this question particularly appealing from the historiographic point of view, geology being by its very nature a well-suited point from which to approach the theme. We argue that there is a long tradition of independence between science and religion, agnosticism and even atheism among local elites. Especially from the eighteenth century onwards, they are usually portrayed as enlightened minds who struggled against religious and political obscurantism. Religion—or, to be more precise, the Roman Catholic Church and its institutions—was usually identified with backwardness, whereas science was seen as the path to progress; consequently men of science usually dissociated their scientific production from religious belief.

  6. Study of the structural and dynamic effects in the FimH adhesin upon α-d-heptyl mannose binding.

    PubMed

    Vanwetswinkel, Sophie; Volkov, Alexander N; Sterckx, Yann G J; Garcia-Pino, Abel; Buts, Lieven; Vranken, Wim F; Bouckaert, Julie; Roy, René; Wyns, Lode; van Nuland, Nico A J

    2014-02-27

    Uropathogenic Escherichia coli cause urinary tract infections by adhering to mannosylated receptors on the human urothelium via the carbohydrate-binding domain of the FimH adhesin (FimHL). Numerous α-d-mannopyranosides, including α-d-heptyl mannose (HM), inhibit this process by interacting with FimHL. To establish the molecular basis of the high-affinity HM binding, we solved the solution structure of the apo form and the crystal structure of the FimHL-HM complex. NMR relaxation analysis revealed that protein dynamics were not affected by the sugar binding, yet HM addition promoted protein dimerization, which was further confirmed by small-angle X-ray scattering. Finally, to address the role of Y48, part of the "tyrosine gate" believed to govern the affinity and specificity of mannoside binding, we characterized the FimHL Y48A mutant, whose conformational, dynamical, and HM binding properties were found to be very similar to those of the wild-type protein. PMID:24476493

  7. Climate change projections for precipitation in Portugal

    NASA Astrophysics Data System (ADS)

    Andrade, C.; Santos, J. A.

    2013-10-01

    The strong irregularity of precipitation in Portugal, which may e.g. trigger severe/extreme droughts and floods, results in a high vulnerability of the country to precipitation inter-annual variability and to its extremes. Furthermore, dryer future climates are projected for Portugal, though there has also been some growing evidence for a strengthening of precipitation extremes. Due to the central role played byprecipitation on many socio-economic sectors and environmental systems, regional climate change assessments for precipitation in Portugal are necessary. This study is focused on analyzing climate change projections for seasonal (3-month) precipitation totals and their corresponding extremes over mainland Portugal. Taking into account the strong seasonality of the precipitation regimes in Portugal, winter (DJF) and summer (JJA) are considered separately. Precipitation datasets generated by a 16-member ensemble of regional climate model experiments from the ENSEMBLES project are used. Percentile-based indices of precipitation are computed and analyzed for a recent past period (1961-2000) and for a near future period (2041-2070). Results for the R5p, R50p and R95p indices highlight significant projected changes in precipitation, with a clear distinction between northwestern Portugal and the rest of the country in both seasons. Overall, precipitation is projected to decrease in both seasons, particularly over northwestern Portugal in winter, despite some significant regional differences. Although precipitation is projected to decrease in most cases, extremely high seasonal precipitations (above the 95th percentile)areexpected to increase in winter.

  8. Initial stages of oxide formation on the Zr surface at low oxygen pressure: An in situ FIM and XPS study.

    PubMed

    Bespalov, I; Datler, M; Buhr, S; Drachsel, W; Rupprechter, G; Suchorski, Y

    2015-12-01

    An improved methodology of the Zr specimen preparation was developed which allows fabrication of stable Zr nanotips suitable for FIM and AP applications. Initial oxidation of the Zr surface was studied on a Zr nanotip by FIM and on a polycrystalline Zr foil by XPS, both at low oxygen pressure (10(-8)-10(-7)mbar). The XPS data reveal that in a first, fast stage of oxidation, a Zr suboxide interlayer is formed which contains three suboxide components (Zr(+1), Zr(+2) and Zr(+3)) and is located between the Zr surface and a stoichiometric ZrO2 overlayer that grows in a second, slow oxidation stage. The sole suboxide layer has been observed for the first time at very early states of the oxidation (oxygen exposure ≤ 4L). The Ne(+) FIM observations are in accord with a two stage process of Zr oxide formation. PMID:25766998

  9. Initial stages of oxide formation on the Zr surface at low oxygen pressure: An in situ FIM and XPS study

    PubMed Central

    Bespalov, I.; Datler, M.; Buhr, S.; Drachsel, W.; Rupprechter, G.; Suchorski, Y.

    2015-01-01

    An improved methodology of the Zr specimen preparation was developed which allows fabrication of stable Zr nanotips suitable for FIM and AP applications. Initial oxidation of the Zr surface was studied on a Zr nanotip by FIM and on a polycrystalline Zr foil by XPS, both at low oxygen pressure (10−8–10−7 mbar). The XPS data reveal that in a first, fast stage of oxidation, a Zr suboxide interlayer is formed which contains three suboxide components (Zr+1, Zr+2 and Zr+3) and is located between the Zr surface and a stoichiometric ZrO2 overlayer that grows in a second, slow oxidation stage. The sole suboxide layer has been observed for the first time at very early states of the oxidation (oxygen exposure ≤4 L). The Ne+ FIM observations are in accord with a two stage process of Zr oxide formation. PMID:25766998

  10. Bordetella pertussis fim3 gene regulation by BvgA: phosphorylation controls the formation of inactive vs. active transcription complexes.

    PubMed

    Boulanger, Alice; Moon, Kyung; Decker, Kimberly B; Chen, Qing; Knipling, Leslie; Stibitz, Scott; Hinton, Deborah M

    2015-02-10

    Two-component systems [sensor kinase/response regulator (RR)] are major tools used by microorganisms to adapt to environmental conditions. RR phosphorylation is typically required for gene activation, but few studies have addressed how and if phosphorylation affects specific steps during transcription initiation. We characterized transcription complexes made with RNA polymerase and the Bordetella pertussis RR, BvgA, in its nonphosphorylated or phosphorylated (BvgA∼P) state at P(fim3), the promoter for the virulence gene fim3 (fimbrial subunit), using gel retardation, potassium permanganate and DNase I footprinting, cleavage reactions with protein conjugated with iron bromoacetamidobenzyl-EDTA, and in vitro transcription. Previous work has shown that the level of nonphosphorylated BvgA remains high in vivo under conditions in which BvgA is phosphorylated. Our results here indicate that surprisingly both BvgA and BvgA∼P form open and initiating complexes with RNA polymerase at P(fim3). However, phosphorylation of BvgA is needed to generate the correct conformation that can transition to competent elongation. Footprints obtained with the complexes made with nonphosphorylated BvgA are atypical; while the initiating complex with BvgA synthesizes short RNA, it does not generate full-length transcripts. Extended incubation of the BvgA/RNA polymerase initiated complex in the presence of heparin generates a stable, but defective species that depends on the initial transcribed sequence of fim3. We suggest that the presence of nonphosphorylated BvgA down-regulates P(fim3) activity when phosphorylated BvgA is present and may allow the bacterium to quickly adapt to the loss of inducing conditions by rapidly eliminating P(fim3) activation once the signal for BvgA phosphorylation is removed. PMID:25624471

  11. A comparison of FUV dayglows measured by STSAT-1/FIMS with the AURIC model in a geomagnetic quiet condition

    NASA Astrophysics Data System (ADS)

    Kam, Hosik; Kim, Yong Ha; Hong, Jun-Seok; Lee, Joon-Chan; Choi, Yeon-Ju; Min, Kyung Wook

    2014-09-01

    The Korea scientific microsatellite, STSAT-1 (Science and Technology Satellite-1), was launched in 2003 and observed far ultraviolet (FUV) airglow from the upper atmosphere with a Far-ultraviolet IMaging Spectrograph (FIMS) at an altitude of 690 km. The FIMS consists of a dual-band imaging spectrograph of 900-1150 Å (S-band) and 1340-1715 Å (L-band). Limb scanning observations were performed only at the S-band, resulting in intensity profiles of OI 989 Å, OI 1026 Å, NII 1085 Å and NI 1134 Å emission lines near the horizon. We compare these emission intensities with those computed by using a theoretical model, the AURIC (Atmospheric Ultraviolet Radiance Integrated Code). The intensities of the OI 1026 Å, NII 1085 Å and NI 1134 Å emissions measured by using the FIMS are overall consistent with the values computed by using AURIC under the thermospheric and solar activity conditions on August 6, 1984, which is close to the FIMS's observation condition. We find that the FIMS dayglow intensity profiles match reasonably well with AURIC intensity profiles for the MSIS90 oxygen atom density profiles within factors of 0.5 and 2. However, the FIMS intensities of the OI 989 Å line are about 2 ˜ 4 times stronger than the AURIC intensities, which is expected because AURIC does not properly simulate resonance scattering of airglow and solar photons at 989 Å by atomic oxygen in the thermosphere. We also find that the maximum tangential altitudes of the oxygen bearing dayglows (OI 989 Å, OI 1026 Å) are higher than those of the nitrogen-bearing dayglows (NII 1085 Å, NI 1134 Å), which is confirmed by using AURIC model calculations. This is expected because the oxygen atoms are distributed at higher altitudes in the thermosphere than the nitrogen molecules. Validations of the qualities of both the FIMS instrument and the AURIC model indicate that AURIC should be updated with improved thermospheric models and with measured solar FUV spectra for better agreement with the

  12. [Carbon monoxide intoxications in Portugal].

    PubMed

    Sá, Márcia Christel; Rodrigues, Rui Paulo; Moura, Daniel

    2011-01-01

    The prevalence of carbon monoxide intoxication in the World shows that this is a common situation. In Portugal, there are no concrete data available in literature and its incidence remains unknown. Currently, the use of hyperbaric oxygen is a valid therapeutic for carbon monoxide poisoning management. However, its effectiveness and its proper handling are still controversial. The first aim of this study was to estimate the incidence of carbon monoxide intoxication in Portugal and to analyze its demographic characteristics. The second objective of this work was to evaluate the possible change in the type of treatment applied in areas near de hyperbaric chamber of Unidade Local de Saúde de Matosinhos, since its opening in June 2006. To achieve these objectives, we conducted a survey on admissions data for carbon monoxide intoxication occured between January first, 2000 and December 31, 2007. These data was collected in seven hospitals and in the Administração Central do Sistema de Saúde, I.P. Nationally, 621 hospitalizations were recorded, which represents an incidence of 5,86/100000 in 8 years. In the seven hospitals, there were 93 hospitalizations due to carbon monoxide intoxication during the same period of time. There was a peak of incidence during winter, between November and March and there was a similar distribution in men (47,3%) and women (52,7%). Since June 2006, date of opening of the hyperbaric chamber, the Unidade Local de Saúde de Matosinhos, E.P.E. recorded a sharp increase in the number of hospitalization for carbon monoxide intoxication. The number of admissions in the 19 months after the chamber opening was double the number of all cases occurred in that institution in the 65 months prior. We concluded that, in Portugal, carbon monoxide intoxication is an uncommon situation but it´s still an important cause of hospitalization. The referral of cases to the Unidade Local de Saúde de Matosinhos, E.P.E. since the opening of hyperbaric chamber

  13. Optometry in Portugal: a historical perspective

    NASA Astrophysics Data System (ADS)

    Teixeira, Eduardo; Baptista, António M. G.; Sousa, Raul A. R. C.

    2011-05-01

    The establishment and development of optometry in Portugal resulted from the committed work of many individuals and institutions. These efforts have had good results in terms of raising the public's awareness of the major role played by optometrists in primary eye care. Back in the late 80's higher education in optometry was started. Ten years ago the results of scientific research on the topic first became available and are now also contributing to the success of optometry in Portugal. In regard to the optometry profession, specific regulations are to be discussed in the national parliament. The Associação de Profissionais Licenciados de Optometria (APLO), as the professional organization representing optometrists in Portugal, has been critically important in this process. This article will present an overview of the history of optometry in Portugal, of change in the foreseeable future and of the APLO's experience and activities.

  14. Astronomy Week in Madeira, Portugal

    NASA Astrophysics Data System (ADS)

    Augusto, P.; Sobrinho, J. L.

    2012-05-01

    The outreach programme Semanas da Astronomia (Astronomy Weeks) is held in late spring or summer on the island of Madeira, Portugal. This programme has been attracting enough interest to be mentioned in the regional press/TV/radio every year and is now, without doubt, the astronomical highlight of the year on Madeira. We believe that this programme is a good case study for showing how to attract the general public to astronomy in a small (population 250 000, area 900 km2) and fairly isolated place such as Madeira. Our Astronomy Weeks have been different each year and have so far included exhibitions, courses, talks, a forum, documentaries, observing sessions (some with blackouts), music and an astro party. These efforts may contribute towards putting Madeira on the map with respect to observational astronomy, and have also contributed to the planned installation of two observatories in the island.

  15. Phytoplankton off the Coast of Portugal

    NASA Technical Reports Server (NTRS)

    2002-01-01

    A large phytoplankton bloom off of the coast of Portugal can be seen in this true-color image taken on April 23, 2002, by the Moderate-resolution Imaging Spectroradiometer (MODIS) aboard NASA's Terra satellite. The bloom is roughly half the size of Portugal and forms a bluish-green cloud in the water. The red spots in northwest Spain denote what are likely small agricultural fires. Image courtesy Jacques Descloitres, MODIS Land Rapid Response Team at NASA GSFC

  16. Lead Optimization Studies on FimH Antagonists: Discovery of Potent and Orally Bioavailable Ortho-substituted Biphenyl Mannosides

    PubMed Central

    Han, Zhenfu; Pinkner, Jerome S.; Ford, Bradley; Chorell, Erik; Crowley, Jan M.; Cusumano, Corinne K.; Campbell, Scott; Henderson, Jeffrey P.; Hultgren, Scott J.; Janetka, James W.

    2012-01-01

    Herein, we describe the X-ray structure-based design and optimization of biaryl mannoside FimH inhibitors. Diverse modifications to the biaryl ring to improve drug-like physical and pharmacokinetic properties of mannosides were assessed for FimH binding affinity based on their effects on hemagglutination and biofilm formation along with direct FimH binding assays. Substitution on the mannoside phenyl ring ortho to the glycosidic bond results in large potency enhancements of several-fold higher than corresponding unsubstituted matched pairs and can be rationalized from increased hydrophobic interactions with the FimH hydrophobic ridge (Ile13) or “tyrosine gate” (Tyr137 and Tyr48) also lined by Ile52. The lead mannosides have increased metabolic stability and oral bioavailability as determined from in vitro PAMPA predictive model of cellular permeability and in vivo pharmacokinetic studies in mice, thereby representing advanced preclinical candidates with promising potential as novel therapeutics for the clinical treatment and prevention of recurring urinary tract infections. PMID:22449031

  17. Structural Analysis of the GGDEF-EAL Domain-Containing c-di-GMP Receptor FimX

    SciTech Connect

    Navarro, M.; De, N; Bae, N; Wang, Q; Sondermann, H

    2009-01-01

    Bacterial pathogenesis involves social behavior including biofilm formation and swarming, processes that are regulated by the bacterially unique second messenger cyclic di-GMP (c-di-GMP). Diguanylate cyclases containing GGDEF and phosphodiesterases containing EAL domains have been identified as the enzymes controlling cellular c-di-GMP levels, yet less is known regarding signal transmission and the targets of c-di-GMP. FimX, a protein from Pseudomonas aeruginosa that governs twitching motility, belongs to a large subfamily containing both GGDEF and EAL domains. Biochemical and structural analyses reveals its function as a high-affinity receptor for c-di-GMP. A model for full-length FimX was generated combining solution scattering data and crystal structures of the degenerate GGDEF and EAL domains. Although FimX forms a dimer in solution via the N-terminal domains, a crystallographic EAL domain dimer suggests modes for the regulation of FimX by c-di-GMP binding. The results provide the structural basis for c-di-GMP sensing via degenerate phosphodiesterases.

  18. Point Mutations in FimH Adhesin of Crohn's Disease-Associated Adherent-Invasive Escherichia coli Enhance Intestinal Inflammatory Response

    PubMed Central

    Dreux, Nicolas; Denizot, Jérémy; Martinez-Medina, Margarita; Mellmann, Alexander; Billig, Maria; Kisiela, Dagmara; Chattopadhyay, Sujay; Sokurenko, Evgeni; Neut, Christel; Gower-Rousseau, Corinne; Colombel, Jean-Frédéric; Bonnet, Richard; Darfeuille-Michaud, Arlette; Barnich, Nicolas

    2013-01-01

    Adherent-invasive Escherichia coli (AIEC) are abnormally predominant on Crohn's disease (CD) ileal mucosa. AIEC reference strain LF82 adheres to ileal enterocytes via the common type 1 pili adhesin FimH and recognizes CEACAM6 receptors abnormally expressed on CD ileal epithelial cells. The fimH genes of 45 AIEC and 47 non-AIEC strains were sequenced. The phylogenetic tree based on fimH DNA sequences indicated that AIEC strains predominantly express FimH with amino acid mutations of a recent evolutionary origin - a typical signature of pathoadaptive changes of bacterial pathogens. Point mutations in FimH, some of a unique AIEC-associated nature, confer AIEC bacteria a significantly higher ability to adhere to CEACAM-expressing T84 intestinal epithelial cells. Moreover, in the LF82 strain, the replacement of fimHLF82 (expressing FimH with an AIEC-associated mutation) with fimHK12 (expressing FimH of commensal E. coli K12) decreased the ability of bacteria to persist and to induce severe colitis and gut inflammation in infected CEABAC10 transgenic mice expressing human CEACAM receptors. Our results highlight a mechanism of AIEC virulence evolution that involves selection of amino acid mutations in the common bacterial traits, such as FimH protein, and leads to the development of chronic inflammatory bowel disease (IBD) in a genetically susceptible host. The analysis of fimH SNPs may be a useful method to predict the potential virulence of E. coli isolated from IBD patients for diagnostic or epidemiological studies and to identify new strategies for therapeutic intervention to block the interaction between AIEC and gut mucosa in the early stages of IBD. PMID:23358328

  19. Silica-gold nanoparticles for atheroprotective management of plaques: results of the NANOM-FIM trial

    NASA Astrophysics Data System (ADS)

    Kharlamov, Alexander N.; Tyurnina, Anastasiya E.; Veselova, Vera S.; Kovtun, Olga P.; Shur, Vladimir Y.; Gabinsky, Jan L.

    2015-04-01

    Background: Atheroregression becomes an attractive target for cardiovascular treatment. Some clinical trials have demonstrated that intensive therapy with rosuvastatin or recombinant ApoA-I Milano can partially reduce the total atheroma volume (TAV) up to 6.38 mm3 or 14.1 mm3 respectively. Our previous bench studies of selected nanotechnologies documented TAV reduction up to an unprecedented 79.4 mm3. Methods: The completed observational three arms (n = 180) first-in-man trial (the NANOM FIM trial) assessed (NCT01270139) the safety and feasibility of two delivery techniques for nanoparticles (NP), and plasmonic photothermal therapy (PPTT). Patients were assigned to receive either (1) nano-intervention with delivery of silica-gold NP in a bioengineered on-artery patch (n = 60), or (2) nano-intervention with delivery of silica-gold iron-bearing NP with targeted micro-bubbles and stem cells using a magnetic navigation system (n = 60) versus (3) stent implantation (n = 60). The primary outcome was TAV at 12 months. Results: The mean TAV reduction at 12 months in the Nano group was 60.3 mm3 (SD 39.5; min 41.9 mm3, max 94.2 mm3; p < 0.05) up to mean 37.8% (95% CI: 31.1%, 51.7%; p < 0.05) plaque burden. The analysis of the event free survival of the ongoing clinical follow-up shows the significantly lower risk of cardiovascular death in the Nano group when compared with others (91.7% vs. 81.7% and 80% respectively; p < 0.05) with no cases of the target lesion-related complications. Conclusions: PPTT using silica-gold NP associated with significant regression of coronary atherosclerosis.Background: Atheroregression becomes an attractive target for cardiovascular treatment. Some clinical trials have demonstrated that intensive therapy with rosuvastatin or recombinant ApoA-I Milano can partially reduce the total atheroma volume (TAV) up to 6.38 mm3 or 14.1 mm3 respectively. Our previous bench studies of selected nanotechnologies documented TAV reduction up to an

  20. Basic Needs and Education in Portugal. The Portugal Project. Document No. XIII.

    ERIC Educational Resources Information Center

    Erasmie, Thord

    The aim of this study was to describe and compare the socio-economic factors that can be expected to influence the education system in Portugal, where urbanization has been very slow. The report examines basic needs in Portugal's 18 districts and makes recommendations about resource allocation to planners of adult education programs. Extensive…

  1. Differentiation of the two major species of Echinacea (E. augustifolia and E. purpurea) using a flow injection mass spectrometric (FIMS) fingerprinting method and chemometric analysis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A rapid, simple, and reliable flow-injection mass spectrometric (FIMS) method was developed to discriminate two major Echinacea species (E. purpurea and E. angustifolia) samples. Fifty-eight Echinacea samples collected from United States were analyzed using FIMS. Principle component analysis (PCA) a...

  2. Effect of a spherical object in 4 electrode Focused Impedance Method (FIM): measurement and simulation

    NASA Astrophysics Data System (ADS)

    Abir, R.; Pettersen, F. J.; Martinsen, O. G.; Rabbani, K. S.

    2013-04-01

    Focused Impedance Method (FIM) gives enhanced localized sensitivity at the centre of a zone defined by a simple system of electrodes, of which a 4-electrode version with electrodes at the corners of a square region has been studied in detail in the present work. The present work studies the effect of a large sphere whose diameter almost equals the dimensions of the central focused zone, or, the Focused Impedance. The sphere is placed at different positions with respect to the centre of the system at the electrode plane. The study has been made using a phantom in which the electrodes are fixed on a side wall while an insulating ball is hung at various positions inside the saline and moved with respect to the electrodes in their vicinity. The same was then simulated by providing appropriate parameters in COMSOL multiphysics, a software package utilizing Finite Element Method, by providing appropriately matching parameters. The measured impedance decreases as the ball is moved away from the centre in the electrode plane or along the depth. The sensitivity also decreases with an increase in electrode spacing. Although the behaviours were similar in both the studies, simulated values by COMSOL deviated from the measured values significantly. It suggests that COMSOL may not give accurate simulations for large objects.

  3. Inhibition profiles of mono- and polyvalent FimH antagonists against 10 different Escherichia coli strains.

    PubMed

    Chalopin, T; Brissonnet, Y; Sivignon, A; Deniaud, D; Cremet, L; Barnich, N; Bouckaert, J; Gouin, S G

    2015-12-14

    Mono- and polyvalent ligands with strong affinities for the mannose-binding adhesin FimH were synthesised, and their anti-adhesive properties against ten E. coli strains were compared in two cell-based assays. The compounds were assessed against the non-pathogenic E. coli K12 and nine strains isolated by coproculture or from patients with osteoarticular infections (OIs), Crohn's disease (CD) and urinary tract infections (UTIs). The results showed that the compounds could inhibit the whole set of bacterial strains but with marked differences in terms of effective concentrations. The relative inhibitory potency of the monovalent compounds was also conserved for the ten strains and in the two assays. These results clearly suggest that a potent monovalent anti-adhesive assessed on a single E. coli strain will probably be effective on a broad range of strains and may treat diverse E. coli infections (OIs, CD and UTIs). In contrast, the polyvalent compounds showed a significant strain-dependancy in preventing E. coli attachment to intestinal cells. The multivalent antiadhesive effect may therefore vary depending on the E. coli strain tested. PMID:26440382

  4. Differentiation of the four major species of cinnamons (C. burmannii, C. verum, C. cassia, and C. loureiroi) using a flow injection mass spectrometric (FIMS) fingerprinting method.

    PubMed

    Chen, Pei; Sun, Jianghao; Ford, Paul

    2014-03-26

    A simple and efficient flow injection mass spectrometric (FIMS) method was developed to differentiate cinnamon (Cinnamomum) bark (CB) samples of the four major species (C. burmannii, C. verum, C. aromaticum, and C. loureiroi) of cinnamon. Fifty cinnamon samples collected from China, Vietnam, Indonesia, and Sri Lanka were studied using the developed FIMS fingerprinting method. The FIMS fingerprints of the cinnamon samples were analyzed using principal component analysis (PCA). The FIMS technique required only 1 min of analysis time per sample. The representative samples from each of the four major species of cinnamon were further examined using an ultrahigh-performance liquid chromatography-high-resolution mass spectrometry system, and the chemical differences between the four species were profiled. The results showed that the 1 min FIMS fingerprinting method successfully differentiated the four cinnamon species studied. PMID:24628250

  5. Differentiation of the Four Major Species of Cinnamons (C. burmannii, C. verum, C. cassia, and C. loureiroi) Using a Flow Injection Mass Spectrometric (FIMS) Fingerprinting Method

    PubMed Central

    2015-01-01

    A simple and efficient flow injection mass spectrometric (FIMS) method was developed to differentiate cinnamon (Cinnamomum) bark (CB) samples of the four major species (C. burmannii, C. verum, C. aromaticum, and C. loureiroi) of cinnamon. Fifty cinnamon samples collected from China, Vietnam, Indonesia, and Sri Lanka were studied using the developed FIMS fingerprinting method. The FIMS fingerprints of the cinnamon samples were analyzed using principal component analysis (PCA). The FIMS technique required only 1 min of analysis time per sample. The representative samples from each of the four major species of cinnamon were further examined using an ultrahigh-performance liquid chromatography–high-resolution mass spectrometry system, and the chemical differences between the four species were profiled. The results showed that the 1 min FIMS fingerprinting method successfully differentiated the four cinnamon species studied. PMID:24628250

  6. Ecole et communaute au Portugal

    NASA Astrophysics Data System (ADS)

    Melo, Alberto

    1980-09-01

    Since 1974 Portugal has experienced some dramatic changes in educational practices at the local level. The school has been opened to the community and the community to the school. Teacher education now includes community studies, designed to prepare teachers for an active role in this process and for the better understanding of the social background of pupils. One new practice is the publication of local newspapers or news-sheets to enhance the understanding of the community by teachers and pupils. The development of a school garden for the cultivation of flowers and vegetables serves as another bridge between school and community. Other examples of community action are adult literacy classes, the creation of voluntary organisations for women, the running of youth clubs, the formation of co-operatives, and local projects like the purchase of an old tram-car to be converted into a library. The introduction of `Civic and Polytechnic Education' for 13-16 year olds was an important innovation. One half-day a week was to be devoted to the integration of the school with the locality, young people's participation in society as agents of change, the linking of study and productive work, and the involvement of young people in the solution of national problems, working from a concrete knowledge of local and regional life. However, since 1976 there has been a more conservative approach, and only in places with a strong consensus amongst teachers has the new relationship between school and community been maintained.

  7. OBSERVATION OF THE FAR-ULTRAVIOLET CONTINUUM BACKGROUND WITH SPEAR/FIMS

    SciTech Connect

    Seon, Kwang-Il; Han, Wonyong; Shinn, Jongho; Kim, Il-Joong; Edelstein, Jerry; Korpela, Eric; Witt, Adolf; Min, Kyoung-Wook; Park, Jae-Woo

    2011-10-01

    We present the general properties of the far-ultraviolet (FUV; 1370-1710 A) continuum background over most of the sky, obtained with the Spectroscopy of Plasma Evolution from Astrophysical Radiation (SPEAR) instrument (also known as FIMS), flown aboard the STSAT-1 satellite mission. We find that the diffuse FUV continuum intensity is well correlated with N{sub HI}, 100 {mu}m, and H{alpha} intensities but anti-correlated with soft X-ray intensity. The correlation of the diffuse background with the direct stellar flux is weaker than the correlation with other parameters. The continuum spectra are relatively flat. However, a weak softening of the FUV spectra toward some sight lines, mostly at high Galactic latitudes, is found not only in direct stellar but also in diffuse background spectra. The diffuse background is relatively softer than the direct stellar spectrum. We also find that the diffuse FUV background averaged over the sky has a bit softer spectrum compared to direct stellar radiation. A map of the ratio of 1370-1520 A to 1560-1710 A band intensity shows that the sky is divided into roughly two parts. However, this map shows a lot of patchy structures on small scales. The spatial variation of the hardness ratio seems to be largely determined by the longitudinal distribution of OB-type stars in the Galactic plane. A correlation of the hardness ratio with the FUV intensity is found at high intensities but an anti-correlation is found at low intensities. We also find evidence that the FUV intensity distribution is log-normal in nature.

  8. Child Poverty in Portugal: Dimensions and Dynamics

    ERIC Educational Resources Information Center

    Bastos, Amelia; Nunes, Francisco

    2009-01-01

    This article analyses the extent and persistence of child poverty in Portugal between 1995 and 2001. Data from the Portuguese component of the European Community Household Panel Survey (ECHP) are used to estimate child poverty rates and children's flows in and out of poverty. The article focuses upon an analysis based on family income and on a set…

  9. Competencia Comunicativa em Portuges (Communicative Competence in Portuguese).

    ERIC Educational Resources Information Center

    Paiva, Ricardo

    A textbook designed to give speech and writing practice to intermediate and advanced students of Portuguese as a second language includes 14 units intended to cover two semesters' work with approximately five hours per week of instruction. The units typically include: a text forming the basis for free conversation and practice of language…

  10. Characterization of FimH adhesins expressed by Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum: reconstitution of mannose-binding properties by single amino acid substitution.

    PubMed

    Kisiela, Dagmara; Sapeta, Anna; Kuczkowski, Maciej; Stefaniak, Tadeusz; Wieliczko, Alina; Ugorski, Maciej

    2005-09-01

    Recombinant FimH adhesins of type 1 fimbriae from Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum, in contrast to those of Salmonella enterica serovar Typhimurium, did not bind to high-mannose oligosaccharides or to human colon carcinoma HT-29 cells. However, mutated FimH proteins from biovar Gallinarum and biovar Pullorum, in which the isoleucine at position 78 was replaced by the threonine found in S. enterica serovar Typhimurium, bound well to glycoproteins carrying high-mannose oligosaccharides and colon carcinoma cells. The loss of sugar-binding properties by biovar Gallinarum and biovar Pullorum FimH adhesins, which are a part of the type 1 fimbriae, is most probably the result of a single T78I mutation, as was proven by site-directed mutagenesis of FimH proteins. PMID:16113346

  11. In silico design of fusion protein of FimH from uropathogenic Escherichia coli and MrpH from Proteus mirabilis against urinary tract infections

    PubMed Central

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Bouzari, Saeid

    2015-01-01

    Background: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) and Proteus mirabilis are the most important pathogens causing UTIs. The FimH from type 1 pili of UPEC and the MrpH from P. mirabilis play critical roles in the UTI process and have presented as ideal vaccine candidates against UTIs. There is no effective vaccine against UTI and the development of an ideal UTI vaccine is required. Materials and Methods: In this study, we planned to design a novel fusion protein of FimH from UPEC and MrpH from P. mirabilis. For this purpose, we modeled fusion protein forms computationally using the Iterative Threading Assembly Refinement (I-TASSER) server and evaluated their interactions with toll-like receptor 4 (TLR4). The best fusion protein was constructed using overlap extension polymerase chain reaction (OE-PCR) and the biological activity of fusion was evaluated by the induction of interleukin-8 (IL-8) in the HT-29 cell line. Results: Our study indicated that based on the Protein Structure Analysis (ProSA)-web and the docking results, MrpH.FimH showed better results than did FimH.MrpH, and it was selected for construction. The results of bioassay on the HT-29 showed that FimH and MrpH.FimH induced significantly higher IL-8 responses than untreated cells or MrpH alone in the cell line tested. Conclusions: In the present study, we designed and constructed the novel fusion protein MrpH.FimH from UPEC and P. mirabilis based on in silico methods. Our bioassay results indicate that the MrpH.FimH fusion protein is active and capable of inducing immune responses. PMID:26605246

  12. Detection of Porphyromonas gingivalis fimA Type I Genotype in Gingivitis by Real-Time PCR–A Pilot Study

    PubMed Central

    Krishnan, Mahalakshmi; Chandrasekaran, S.C.

    2016-01-01

    Introduction Published literature till date reveals a high prevalence of Porphyromonas gingivalis fimA type I genotype among healthy subjects. Quite a few studies have reported its prevalence also in periodontitis patients. Nevertheless incidence of this genotype in gingivitis is lacking in adult population. Aim The present study was chosen to detect P. gingivalis fimA type I genotype among chronic gingivitis patients. Materials and Methods A total of 46 subgingival plaque samples collected from chronic marginal gingivitis (n=23) and chronic periodontitis subjects (control group) (n=23) were subjected to Real-Time Polymerase Chain Reaction to detect the P. gingivalis fimA type I gene. Statistical analysis was performed using chi-square test. Results Prevalence of P. gingivalis fimA type I gene among chronic periodontitis and chronic gingivitis patients were 8.7% and 30.4% respectively. P. gingivalis fimA type I genotype prevalence was found to be statistically insignificant between the two study groups (p=0.135). Conclusion The avirulent P. gingivalis fimA type I genotype, occurred in high prevalence among chronic gingivitis patients, while its presence was low in chronic periodontitis patients. Presence of this avirulent genotype in chronic marginal gingivitis signifies its reversible condition. PMID:27504406

  13. Multivariate statistical analysis of wildfires in Portugal

    NASA Astrophysics Data System (ADS)

    Costa, Ricardo; Caramelo, Liliana; Pereira, Mário

    2013-04-01

    Several studies demonstrate that wildfires in Portugal present high temporal and spatial variability as well as cluster behavior (Pereira et al., 2005, 2011). This study aims to contribute to the characterization of the fire regime in Portugal with the multivariate statistical analysis of the time series of number of fires and area burned in Portugal during the 1980 - 2009 period. The data used in the analysis is an extended version of the Rural Fire Portuguese Database (PRFD) (Pereira et al, 2011), provided by the National Forest Authority (Autoridade Florestal Nacional, AFN), the Portuguese Forest Service, which includes information for more than 500,000 fire records. There are many multiple advanced techniques for examining the relationships among multiple time series at the same time (e.g., canonical correlation analysis, principal components analysis, factor analysis, path analysis, multiple analyses of variance, clustering systems). This study compares and discusses the results obtained with these different techniques. Pereira, M.G., Trigo, R.M., DaCamara, C.C., Pereira, J.M.C., Leite, S.M., 2005: "Synoptic patterns associated with large summer forest fires in Portugal". Agricultural and Forest Meteorology. 129, 11-25. Pereira, M. G., Malamud, B. D., Trigo, R. M., and Alves, P. I.: The history and characteristics of the 1980-2005 Portuguese rural fire database, Nat. Hazards Earth Syst. Sci., 11, 3343-3358, doi:10.5194/nhess-11-3343-2011, 2011 This work is supported by European Union Funds (FEDER/COMPETE - Operational Competitiveness Programme) and by national funds (FCT - Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124-FEDER-022692, the project FLAIR (PTDC/AAC-AMB/104702/2008) and the EU 7th Framework Program through FUME (contract number 243888).

  14. The Shaft of the Type 1 Fimbriae Regulates an External Force to Match the FimH Catch Bond

    PubMed Central

    Zakrisson, Johan; Wiklund, Krister; Axner, Ove; Andersson, Magnus

    2013-01-01

    Type 1 fimbriae mediate adhesion of uropathogenic Escherichia coli to host cells. It has been hypothesized that due to their ability to uncoil under exposure to force, fimbriae can reduce fluid shear stress on the adhesin-receptor interaction by which the bacterium adheres to the surface. In this work, we develop a model that describes how the force on the adhesin-receptor interaction of a type 1 fimbria varies as a bacterium is affected by a time-dependent fluid flow mimicking in vivo conditions. The model combines in vivo hydrodynamic conditions with previously assessed biomechanical properties of the fimbriae. Numerical methods are used to solve for the motion and adhesion force under the presence of time-dependent fluid profiles. It is found that a bacterium tethered with a type 1 pilus will experience significantly reduced shear stress for moderate to high flow velocities and that the maximum stress the adhesin will experience is limited to ∼120 pN, which is sufficient to activate the conformational change of the FimH adhesin into its stronger state but also lower than the force required for breaking it under rapid loading. Our model thus supports the assumption that the type 1 fimbria shaft and the FimH adhesin-receptor interaction are optimized to each other, and that they give piliated bacteria significant advantages in rapidly changing fluidic environments. PMID:23708354

  15. Stamina pistilloida, the Pea ortholog of Fim and UFO, is required for normal development of flowers, inflorescences, and leaves.

    PubMed

    Taylor, S; Hofer, J; Murfet, I

    2001-01-01

    Isolation and characterization of two severe alleles at the Stamina pistilloida (Stp) locus reveals that Stp is involved in a wide range of developmental processes in the garden pea. The most severe allele, stp-4, results in flowers consisting almost entirely of sepals and carpels. Production of ectopic secondary flowers in stp-4 plants suggests that Stp is involved in specifying floral meristem identity in pea. The stp mutations also reduce the complexity of the compound pea leaf, and primary inflorescences often terminate prematurely in an aberrant sepaloid flower. In addition, stp mutants were shorter than their wild-type siblings due to a reduction in cell number in their internodes. Fewer cells were also found in the epidermis of the leaf rachis of stp mutants. Examination of the effects of stp-4 in double mutant combinations with af, tl, det, and veg2-2-mutations known to influence leaf, inflorescence, and flower development in pea-suggests that Stp function is independent of these genes. A synergistic interaction between weak mutant alleles at Stp and Uni indicated that these two genes act together, possibly to regulate primordial growth. Molecular analysis revealed that Stp is the pea homolog of the Antirrhinum gene Fimbriata (Fim) and of UNUSUAL FLORAL ORGANS (UFO) from Arabidopsis. Differences between Fim/UFO and Stp mutant phenotypes and expression patterns suggest that expansion of Stp activity into the leaf was an important step during evolution of the compound leaf in the garden pea. PMID:11158527

  16. Transurethral instillation with fusion protein MrpH.FimH induces protective innate immune responses against uropathogenic Escherichia coli and Proteus mirabilis.

    PubMed

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Bouzari, Saeid

    2016-06-01

    Urinary tract infections (UTIs) are among the most common infections in human. Innate immunity recognizes pathogen-associated molecular patterns (PAMPs) by Toll-like receptors (TLRs) to activate responses against pathogens. Recently, we demonstrated that MrpH.FimH fusion protein consisting of MrpH from Proteus mirabilis and FimH from Uropathogenic Escherichia coli (UPEC) results in the higher immunogenicity and protection, as compared with FimH and MrpH alone. In this study, we evaluated the innate immunity and adjuvant properties induced by fusion MrpH.FimH through in vitro and in vivo methods. FimH and MrpH.FimH were able to induce significantly higher IL-8 and IL-6 responses than untreated or MrpH alone in cell lines tested. The neutrophil count was significantly higher in the fusion group than other groups. After 6 h, IL-8 and IL-6 production reached a peak, with a significant decline at 24 h post-instillation in both bladder and kidney tissues. Mice instilled with the fusion and challenged with UPEC or P. mirabilis showed a significant decrease in the number of bacteria in bladder and kidney compared to control mice. The results of these studies demonstrate that the use of recombinant fusion protein encoding TLR-4 ligand represents an effective vaccination strategy that does not require the use of a commercial adjuvant. Furthermore, MrpH.FimH was presented as a promising vaccine candidate against UTIs caused by UPEC and P. mirabilis. PMID:26918627

  17. Population diversity of Theileria annulata in Portugal.

    PubMed

    Gomes, Jacinto; Salgueiro, Patrícia; Inácio, João; Amaro, Ana; Pinto, João; Tait, Andy; Shiels, Brian; Pereira da Fonseca, Isabel; Santos-Gomes, Gabriela; Weir, William

    2016-08-01

    The tick-borne protozoan parasite Theileria annulata causes tropical theileriosis, a severe disease of cattle that occurs across the Mediterranean littoral, the Middle East and Southern Asia. In the Mediterranean region, the disease has long been perceived as being a constraint to livestock production in North Africa and Turkey but was believed to have minimal impact in Southern European countries. It has recently been demonstrated that in Southern Portugal the prevalence of T. annulata is approximately 30%. While the population genetics of the parasite and the multiplicity of infection in the bovine host have been studied in a number of countries, no information is currently available on the composition of the parasite population in Southern Europe or its relationship to populations in bordering regions. A parasite genotyping system, based on micro- and mini-satellite amplification, was used to perform genetic analysis of T. annulata populations from T. annulata infected cattle in twelve farms in Southern Portugal. A diversity of genotypes and a high multiplicity of infection were found, suggesting that the parasite possesses a panmictic population in this region. In comparison with genotypes found in Tunisia and Turkey, parasites from Portugal form a genetically distinct group and show lower genetic diversity. PMID:27109468

  18. Municipal solid waste disposal in Portugal

    SciTech Connect

    Magrinho, Alexandre; Didelet, Filipe; Semiao, Viriato . E-mail: ViriatoSemiao@ist.utl.pt

    2006-07-01

    In recent years municipal solid waste (MSW) disposal has been one of the most important environmental problems for all of the Portuguese regions. The basic principles of MSW management in Portugal are: (1) prevention or reduction, (2) reuse, (3) recovery (e.g., recycling, incineration with heat recovery), and (4) polluter-pay principle. A brief history of legislative trends in waste management is provided herein as background for current waste management and recycling activities. The paper also presents and discusses the municipal solid waste management in Portugal and is based primarily on a national inquiry carried out in 2003 and directed to the MSW management entities. Additionally, the MSW responsibility and management structure in Portugal is presented, together with the present situation of production, collection, recycling, treatment and elimination of MSW. Results showed that 96% of MSW was collected mixed (4% was separately collected) and that 68% was disposed of in landfill, 21% was incinerated at waste-to-energy plants, 8% was treated at organic waste recovery plants and 3% was delivered to sorting. The average generation rate of MSW was 1.32 kg/capita/day.

  19. [Can the classification of patients for phase B, C and D of neurological rehabilitation be done by FIM(tm) scores?].

    PubMed

    Nosper, M

    2002-02-01

    The classification of patients for phase B, C and D of neurological rehabilitation follows the suggestions of the Bundesarbeitsgemeinschaft für Rehabilitation (BAR) based on defined clinical criteria and on neurological rehabilitation assessment. The focus of this study is to define the intervals of the complete FIM(tm)-index, intervals covered empirically as well as by evaluations of physicians, that permit utmost accuracy in assigning patients to phase B, C and D of neurological rehabilitation. Therefore, data records of 3686 patients from 4 neurological rehabilitation centres were evaluated. The patients' functional autonomy was classified by FIM(tm) on admission, in intervals of 14 days and at discharge, at the same time all patients in addition were assigned to phases B, C or D by the rehabilitation centre physicians. Statistical analysis of a total of 11,247 links of the phase classifications and FIM-indexes at 6 measurement points showed that correct correlation to phase B, C and D could be obtained on average in 79 to 89 % of the cases, based on the assumption that 18 - 36 points of the FIM-index assign to phase B, 37 - 90 points to phase C and 91 - 126 points to phase D. Discrimination between phases B and C could be obtained accurately in an average of 84 %, discrimination between phases C and D in an average of 89 %. Conformance of the FIM-intervals with TAR-based groups of care efforts compared to the evaluation by physicians indicate that the FIM(tm) represents the need for care with greater validity. If assignment to phases B, C and D would have been done on the basis of the FIM-index instead of evaluation by a physician, 8,9 % fewer patients would on admission have been classified for phase C but, instead, 4,5 % more patients for phase B and 4,4 % for phase D. In case of using the FIM-intervals for classification, 12,1 % more phase B patients could have changed to phases C or D. Of the phase C cases, 17,7 % could have been transferred to phase D

  20. Two autonomous structural modules in the fimbrial shaft adhesin FimA mediate Actinomyces interactions with streptococci and host cells during oral biofilm development

    SciTech Connect

    Mishra, Arunima; Devarajan, Bharanidharan; Reardon, Melissa E.; Dwivedi, Prabhat; Krishnan, Vengadesan; Cisar, John O.; Das, Asis; Narayana, Sthanam V.L.; Ton-That, Hung

    2011-09-06

    By combining X-ray crystallography and modelling, we describe here the atomic structure of distinct adhesive moieties of FimA, the shaft fimbrillin of Actinomyces type 2 fimbriae, which uniquely mediates the receptor-dependent intercellular interactions between Actinomyces and oral streptococci as well as host cells during the development of oral biofilms. The FimA adhesin is built with three IgG-like domains, each of which harbours an intramolecular isopeptide bond, previously described in several Gram-positive pilins. Genetic and biochemical studies demonstrate that although these isopeptide bonds are dispensable for fimbrial assembly, cell-cell interactions and biofilm formation, they contribute significantly to the proteolytic stability of FimA. Remarkably, FimA harbours two autonomous adhesive modules, which structurally resemble the Staphylococcus aureus Cna B domain. Each isolated module can bind the plasma glycoprotein asialofetuin as well as the polysaccharide receptors present on the surface of oral streptococci and epithelial cells. Thus, FimA should serve as an excellent paradigm for the development of therapeutic strategies and elucidating the precise molecular mechanisms underlying the interactions between cellular receptors and Gram-positive fimbriae.

  1. A Decision Support Matrix (DSM) approach to mapping the impacts of flooding mitigation using a Flood Impact Model (FIM)

    NASA Astrophysics Data System (ADS)

    Owen, Gareth; Quinn, Paul; ODonnell, Greg

    2014-05-01

    The village of Great Ayton on the River Leven catchment (35 km2), UK, has been impacted by a number of floods in recent years. A flood management scheme based on the installation of Natural Flood Management (NFM) interventions has commenced to deal with this issue. Evidence gathered within the subtributaries (less than 10 km2) demonstrates the potential for NFM techniques to have some effect on the local flood peaks. However, the problem arises as to how to upscale these results to the catchment scale, which involves consideration of the timing of the delivery of water from the landscape units, interactions flows at confluences, the spatial patterns of rainfall, antecedent conditions and so forth. This study uses a novel Flood Impact Model (FIM) and a nested network of observed flow measurements. Essentially the approach involves the disaggregation of the outlet hydrograph by making assumptions about the spatial distribution of runoff generation. The channel network is parameterised through a comparison of the timing of observed hydrographs at a number of nested locations within the catchment. The greater the number of flow recording instruments installed within the catchment the better network is thus defined. The Environment Agency, North Yorkshire Moor National Park, the Forestry Commission (England), local farming and town residents formed a catchment consortium for the Leven. An initial catchment plan was created and local evidence was gathered, with a flow instrumentation network installed. A simple visual Decision Support Matrix (DSM) was then used to convey the likely impacts that arise from any area undergoing future flood management measures. The DSM encourages end users to create a NFM scheme by studying the degree to which runoff can be altered locally and how that flow will be propagated through the network to the point of impact. The model contains a simple runoff generator based on the UK Flood Estimation Handbook (FEH) method (though any runoff

  2. OECD Review of the Secondary School Modernisation Programme in Portugal

    ERIC Educational Resources Information Center

    Blyth, Alastair

    2010-01-01

    On 26 February in Lisbon a team from the OECD's Centre for Effective Learning Environments (CELE) presented its first review of a national school building programme. The school building renovation programme in Portugal, which began in March 2007, involves the renovation of 332 schools by 2015. However, Portugal plans to complete 205 by the end of…

  3. Immigration and Supplementary Ethnic Schooling: Ukrainian Students in Portugal

    ERIC Educational Resources Information Center

    Tereshchenko, Antonina; Grau Cárdenas, Valeska Valentina

    2013-01-01

    Immigration from Eastern European countries to Portugal is a recent phenomenon. Within the last decade, economic migrants from Ukraine, Russia, Romania and Moldova set up a number of supplementary schools across the country. No academic attention has been given to the phenomenon of supplementary ethnic schools in Portugal, whilst there is a…

  4. Characterization of organic and conventional sweet basil leaves using chromatographic and flow-injection mass spectrometric (FIMS) fingerprints combined with principal component analysis

    PubMed Central

    Lu, Yingjian; Gao, Boyan; Chen, Pei; Charles, Denys; Yu, Liangli (Lucy)

    2014-01-01

    Sweet basil, Ocimum basilicum., is one of the most important and wildly used spices and has been shown to have antioxidant, antibacterial, and anti-diarrheal activities. In this study, high performance liquid chromatographic (HPLC) and flow-injection mass spectrometric (FIMS) fingerprinting techniques were used to differentiate organic and conventional sweet basil leaf samples. Principal component analysis (PCA) of the fingerprints indicated that both HPLC and FIMS fingerprints could effectively detect the chemical differences in the organic and conventional sweet basil leaf samples. This study suggested that the organic basil sample contained greater concentrations of almost all the major compounds than its conventional counterpart on a per same botanical weight basis. The FIMS method was able to rapidly differentiate the organic and conventional sweet basil leaf samples (1 min analysis time), whereas the HPLC fingerprints provided more information about the chemical composition of the basil samples with a longer analytical time. PMID:24518341

  5. Sensitivity of dust emissions to aerosol feedback and the impact of dust loading on climate forcing with varied resolutions using FIM-Chem

    NASA Astrophysics Data System (ADS)

    Zhang, Li; Grell, Georg; Henze, Daven; Mckeen, Stuart; Sun, Shan; Li, Haiqin

    2016-04-01

    Meteorological conditions directly impact aerosol loading, especially dust emissions. Variations in dust emissions on the other hand, will also impact meteorology and climate through direct and indirect aerosol forcing. To study these impacts in more detail we use the global Flow-following finite-volume Icosahedra Model (FIM, http://fim.noaa.gov/), a new global weather prediction model currently under development in the Global Systems Division of NOAA/ESRL, as it is coupled online with the aerosol modules from the Goddard Gobal Ozone Chemistry Aerosol Radiation and Transport (GOCART) model (FIM-Chem). FIM-Chem includes direct and semi direct feedback, and uses the dust schemes of GOCART and the Air Force Weather Agency (AFWA). FIM-Chem is able to investigate the contribution of climate feedbacks to simulated hyperspectral data by considering a range of simulations with different dust emissions and different levels of aerosol feedbacks enabled at four different spatial resolutions. The emitted dust flux and total emissions are highly depending on the wind, soil moisture and model resolution. We compare the dust emissions by including and excluding the aerosol radiative feedback in the simulations to quantify the sensitivity of dust emissions to aerosol feedback. The results show that all aerosol-induced dust emissions increase about 10% globally, which is mainly dominated by the contributions of anthropogenic black carbon (EC) aerosol. While the dust-induced percentage changes of dust emissions are about -5.5%, that indicates reduction effect globally. Also, the simulations based on different resolutions of 240x240 km, 120x120 km, 60x60 km and 30x30 km are performed to test the impacts of model resolution on total dust emissions. By comparing the dust emission sensitivity to aerosol feedback and model resolution, we can estimate the uncertainty of model resolution versus aerosol feedback. We also conduct FIM-Chem simulations to investigate the sensitivity of dust

  6. Prevalence of Porphyromonas gingivalis fimA genotypes in the peri-implant sulcus of Koreans assessed using a new primer

    PubMed Central

    2016-01-01

    Purpose Porphyromonas gingivalis fimA is a virulence factor associated with periodontal diseases, but its role in the pathogenesis of peri-implantitis remains unclear. We aimed to evaluate the relationship between the condition of peri-implant tissue and the distribution of P. gingivalis fimA genotypes in Koreans using a new primer. Methods A total of 248 plaque samples were taken from the peri-implant sulci of 184 subjects. The control group consisted of sound implants with a peri-implant probing depth (PD) of 5 mm or less with no bleeding on probing (BOP). Test group I consisted of implants with a peri-implant PD of 5 mm or less and BOP, and test group II consisted of implants with a peri-implant PD of more than 5 mm and BOP. DNA was extracted from each sample and analyzed a using a polymerase chain reaction (PCR) with P. gingivalis-specific primers, followed by an additional PCR assay to differentiate the fimA genotypes in P. gingivalis-positive subjects. Results The Prevalence of P. gingivalis in each group did not significantly differ (P>0.05). The most predominant fimA genotype in all groups was type II. The prevalence of type Ib fimA was significantly greater in test group II than in the control group (P<0.05). Conclusions The fimA type Ib genotype of P. gingivalis was found to play a critical role in the destruction of peri-implant tissue, suggesting that it may be a distinct risk factor for peri-implantitis. PMID:26937292

  7. Phylogenetic analysis of Dichelobacter nodosus serogroup-specific fimA gene from ovine footrot in Andhra Pradesh

    PubMed Central

    Kumar, N. Vinod; Karthik, A.; Vijayalakhsmi, S.; Sreenivasulu, D.

    2015-01-01

    Aim: Identification of different serogroups of Dichelobacter nodosus prevailing in the region and to understand the degree of genetic heterogeneities among the different isolates of D. nodosus. Materials and Methods: A total of 150 exudate samples of footrot lesions with a lesion score of 2-4 were collected from naturally infected sheep. The samples were screened by polymerase chain reaction (PCR) targeting D. nodosus specific 16srRNA. Of 150 samples screened, 70 samples were found to be positive. The positive samples were attempted for isolation of D. nodosus, out of which 16 isolates were recovered. All the isolates were subjected to serogrouping by multiplex PCR targeting fimA gene using A-I serogroup specific primers. Results: Of 16 isolates, 7 (43.75%) isolates were serogroup B, 4 (25.00%) isolates were serogroup A, 3 isolates (18.75%) were serogroup I and 2 (12.5%) isolates yielded both serogroup A and B. phylogenetic analysis was performed using neighbor-joining algorithm of the ClustelX2 software in order to study whether the serogroups isolated in the present investigation differed genetically from other published serogroups. The fimA gene sequence of present isolates of serogroups A, B, and I were segregated into three distinct groups with high bootstrap values. The serogroup B clustered with Australian isolate of serotype B1 suggesting high genetic similarity of the present isolate with serotype B1. Conclusions: The clinical samples were collected from suspected outbreaks of footrot and identified the prevalence of D. nodosus by PCR targeting 16srRNA gene. Identified serogroups A, B, and I from different districts of Andhra Pradesh. The phylogenetic analysis will help for the tentative identification of serotypes present in the serogroup and to understand the degree of genetic heterogeneities among the different isolates of D. nodosus. PMID:27047136

  8. Portugal takes step back on abortion legalization.

    PubMed

    1998-07-01

    According to international press reports, a law that would have allowed Portuguese women abortions through the 10th week of pregnancy and into the 16th week if their physical or mental health was at risk has been rescinded after a referendum to determine the statute's future was voided because of low voter turnout. Passed in February, the law was a liberalization of Portugal's strict anti-abortion laws, which ban all abortions except for narrowly defined medical reasons or in the case of rape (and those are permitted only until the 12th week of pregnancy). Because the issue is such a controversial one, politicians had turned to a national referendum asking Portuguese voters to overturn or ratify the new law. The referendum was the first in the country since the end of its right-wing dictatorship in 1974, and 50% participation was required. Only 31.5% of the country's 8.5 million eligible voters went to the polls on June 28. Of those voting, 50.9% voted against the liberalized new legislation. Sunny weather and World Cup soccer matches were both pointed to as reasons for the low turnout. Officials estimate there are some 20,000 illegal abortions annually in Portugal. Abortion-rights activists in the mostly Roman-Catholic country say hospitals see roughly 10,000 women a year suffering from complications from illegal abortions, and that at least 800 women die each year from the procedure. In the next day's Diario de Noticias, a daily paper in Portugal, the entire front page was filled with a giant question mark. "What now, lawmakers?" the headline read. PMID:12293809

  9. Interpersonal violence among immigrants in Portugal.

    PubMed

    Dias, Sónia; Fraga, Sílvia; Barros, Henrique

    2013-02-01

    To assess prevalence of interpersonal violence among a mixed gender sample of immigrants in Portugal, describing the type of violence and associated factors. A cross-sectional study was conducted between October 2008 and May 2009, evaluating a sample of 702 immigrants residing in the Lisbon region. Information was obtained by trained interviewers using a structured questionnaire. Overall, 15.1 % (15.5 % females and 14.7 % males; p = 0.844) of the immigrants reported to be victims of at least one episode of violence during the last year, regardless of which type of violence was involved. The prevalence of intimate-partner violence was 4.1 %, and it was significantly higher among women than men (7.1 % vs. 0.9 %, respectively, p < 0.001). Women who reported being victims of violence during the previous year stated that the episodes occurred more often at home (54.4 %) with the partner as the perpetrator (43.9 %). On the other hand, male victims stated that the violent episodes occurred mostly in public spaces (40.8 %); men indicated that the perpetrator was frequently a stranger (28.6 %) or a co-worker (18.4 %). Violence is a frequent problem among both female and male immigrants living in Portugal, with different gender patterns regarding the perpetrators and settings of abuse. PMID:22618354

  10. Space Radar Image of Lisbon, Portugal

    NASA Technical Reports Server (NTRS)

    1994-01-01

    This radar image of Lisbon, Portugal illustrates the different land use patterns that are present in coastal Portugal. Lisbon, the national capital, lies on the north bank of the Rio Tejo where the river enters the Atlantic Ocean. The city center appears as the bright area in the center of the image. The green area west of the city center is a large city park called the Parque Florestal de Monsanto. The Lisbon Airport is visible east of the city. The Rio Tejo forms a large bay just east of the city. Many agricultural fields are visible as a patchwork pattern east of the bay. Suburban housing can be seen on the southern bank of the river. Spanning the river is the Ponte 25 de Abril, a large suspension bridge similar in architecture to San Francisco's Golden Gate Bridge. The image was acquired on April 19, 1994 and is centered at 38.8 degrees north latitude, 9.2 degrees west longitude. North is towards the upper right. The image is 50 kilometers by 30 kilometers (31 miles by 19 miles). The colors in this image represent the following radar channels and polarizations: red is L-band, horizontally transmitted and received; green is L-band, horizontally transmitted and vertically received; and blue is C-band, horizontally transmitted and vertically received. SIR-C/X-SAR, a joint mission of the German, Italian, and the United States space agencies, is part of NASA's Mission to Planet Earth.

  11. Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From Patients With Urinary Tract Infection

    PubMed Central

    Rahdar, Masoud; Rashki, Ahmad; Miri, Hamid Reza; Rashki Ghalehnoo, Mehdi

    2015-01-01

    Background: Uropathogenic Escherichia coli (UPEC) with its virulence factors is the most prevalent cause of urinary tract infection (UTI). Objectives; This study aimed to determine the occurrence of fim, pap, sfa, and afa genes among 100 UPEC isolates collected from patients diagnosed with UTI. Materials and Methods A total of 100 UPEC isolates were obtained from urine samples of patients with UTI. The prevalence of 5 virulence genes encoding type 1 fimbriae (fimH), pili associated with pyelonephritis (pap), S and F1C fimbriae (sfa and foc) and afimbrial adhesins (afa) were determined through PCR method. We also investigated the phylogenetic background of all isolates. In addition, the distribution of adhesin-encoding operons between the phylogroups was assessed. Results: The prevalence of genes encoding for fimbrial adhesive systems was 95% for fim, 57% for pap, 16% for foc, and 81% for sfa. The operons encoding for afa afimbrial adhesins were identified in 12% of isolates. The various combinations of detected genes were designated as virulence patterns. The fim gene, which occurred in strains from all phylogenetic groups (A, B1, B2, and D) was evaluated and no significant differences were found among these groups. Conversely, significant differences were observed in relation to pap, afa, foc, and sfa operons. Conclusions: These results indicate that the PCR method is a powerful genotypic assay for the detection of adhesin-encoding operons. Thus, this assay can be recommended for clinical use to detect virulent urinary E. coli strains, as well as epidemiological studies. PMID:26464770

  12. Differentiation of the four major types (C. Burmannii, C. Verum, C. cassia, And C. Loureiroi) of cinnamons using a flow-injection mass spectrometric (FIMS) fingerprinting method

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A simple and efficient flow-injection mass spectrometric (FIMS) method was developed to differentiate cinnamon (Cinnamomum) bark (CB) samples of the four major species (C. burmannii, C. verum, C. aromaticum, and C. loureiroi) of cinnamon. Fifty cinnamon samples collected from China, Vietnam, Indon...

  13. Improved detection of low vapor pressure compounds in air by serial combination of single-sided membrane introduction with fiber introduction mass spectrometry (SS-MIMS-FIMS).

    PubMed

    Cotte-Rodríguez, Ismael; Handberg, Eric; Noll, Robert J; Kilgour, David P A; Cooks, R Graham

    2005-05-01

    The use of two methods in tandem, single-sided membrane introduction mass spectrometry (SS-MIMS) and fiber introduction mass spectrometry (FIMS), is presented as a technique for field analysis. The combined SS-MIMS-FIMS technique was employed in both a modified commercial mass spectrometer and a miniature mass spectrometer for the selective preconcentration of the explosive simulant o-nitrotoluene (ONT) and the chemical warfare agent simulant, methyl salicylate (MeS), in air. A home-built FIMS inlet was fabricated to allow introduction of the solid-phase microextraction (SPME) fiber into the mass spectrometer chamber and subsequent desorption of the trapped compounds using resistive heating. The SS-MIMS preconcentration system was also home-built from commercial vacuum parts. Optimization experiments were done separately for each preconcentration system to achieve the best extraction conditions prior to use of the two techniques in combination. Improved limits of detection, in the low ppb range, were observed for the combination compared to FIMS alone, using several SS-MIMS preconcentration cycles. The SS-MIMS-FIMS response for both instruments was found to be linear over the range 50 to 800 ppb. Other parameters studied were absorption time profiles, effects of sample flow rate, desorption temperature, fiber background, memory effects, and membrane fatigue. This simple, sensitive, accurate, robust, selective, and rapid sample preconcentration and introduction technique shows promise for field analysis of low vapor pressure compounds, where analyte concentrations will be extremely low and the compounds are difficult to extract from a matrix like air. PMID:15852137

  14. Cellular immunity survey against urinary tract infection using pVAX/fimH cassette with mammalian and wild type codon usage as a DNA vaccine

    PubMed Central

    Bagherpour, Ghasem; Khoramabadi, Nima; Fallah Mehrabadi, Jalil; Mahdavi, Mehdi; Halabian, Raheleh; Amin, Mohsen; Izadi Mobarakeh, Jalal; Einollahi, Behzad

    2014-01-01

    Purpose FimH (the adhesion fragment of type 1 fimbriae) is implicated in uropathogenic Escherichia coli (UPEC) attachment to epithelial cells through interaction with mannose. Recently, some studies have found that UPEC can thrive intracellularly causing recurrent urinary tract infection (UTI). Almost all vaccines have been designed to induce antibodies against UPEC. Yet, the humoral immune response is not potent enough to overcome neither the primary UTI nor recurrent infections. However, DNA vaccines offer the possibility of inducing cell mediated immune responses and may be a promising preventive tool. Materials and Methods In this study, we employed two different open reading frames within mammalian (mam) and wild type (wt) codons of fimH gene. Optimized fragments were cloned in pVAX-1. Expression of the protein in COS-7 was confirmed by western blot analysis after assessing pVAX/fimH(mam) and pVAX/fimH(wt). The constructs were injected to BALB/c mice at plantar surface of feet followed by electroporation. Results The mice immunized with both constructs following booster injection with recombinant FimH showed increased interferon-γ and interleukin-12 responses significantly higher than non-immunized ones (p<0.05). The immunized mice were challenged with UPEC and then the number of bacteria recovered from the immunized mice was compared with the non-immunized ones. Decreased colony count in immunized mice along with cytokine responses confirmed the promising immune response by the DNA vaccines developed in this study. Conclusion In conclusion, DNA vaccines of UPEC proteins may confer some levels of protection which can be improved by multiple constructs or boosters. PMID:25003092

  15. Occupational exposure in Portugal in 1999.

    PubMed

    Alves, J G; Martins, M B; Amaral, E M

    2001-01-01

    This study reports the occupational radiation doses for external exposure received in 1999 by the radiation workers monitored by the Radiological Protection and Nuclear Safety Department (DPRSN) in Portugal. Occupational exposures arise from conventional industry, research laboratories, the health or medical sector, and mining. There are no nuclear power plants in the country. There are two dosimetry systems running simultaneously at DPRSN, one based on film dosimetry and the other on thermoluminescence dosimetry (TLD). In 1999, 8400 persons were monitored, 3100 with film and 5300 with TLD and the data presented in this report were obtained by using both technologies. The annual mean effective doses received from external radiation in the different fields of activity and the distribution of the annual effective dose by dose intervals are presented. The collective annual dose by field of activity is estimated and the contribution to the total annual collective dose is determined. PMID:11586752

  16. The determinants of lottery sales in Portugal.

    PubMed

    Kaizeler, Maria João; Faustino, Horácio C; Marques, Rafael

    2014-09-01

    In sum, this paper examines the core determinants of lottery sales in Portugal. With panel data analysis covering 18 Portuguese districts and 5 years, this study explains the variations in a district's per-capita lottery sales in terms of key theoretical hypotheses such as per capita district income, age, education index, gender and religion. This paper concludes that the richer Portuguese districts spend more than the poorer districts and there is an inverted-U relationship between lottery sales and per capita income. The paper also concludes that married Catholic citizens spend more on lottery products than others and that the level of education returns a negative effect on lottery spending. PMID:23760689

  17. KLIMHIST: A Project on Historical Climatology in Portugal

    NASA Astrophysics Data System (ADS)

    Fragoso, Marcelo; João Alcoforado, Maria; Santos, João A.

    2013-04-01

    Climatic variability from the beginning of regular meteorological observations is now acknowledged. However, climate change prior to 1900 is far from being well known in Portugal, except for the 1675-1800 period in Southern Portugal. An interdisciplinary team is working in the frame of the KLIMHIST PROJECT ("Reconstruction and model simulations of past climate in Portugal using documentary and early instrumental sources, 17th-19th century)", since May 2012. The main objectives of the project are: (i) to contribute to the creation of a long-term history of climate in Portugal by producing databases of documentary evidence and of instrumental data since 1645, a period of natural climate variability that includes the Maunder Minimum and the Dalton Minimum; (ii) to search systematically for the first simultaneous documentary and instrumental data in order to calibrate the series; (iii) to analyse simulated multi-decadal trends over Portugal generated by climate models; (iv) to compare results with those obtained from dendroclimatology and from geothermal studies regarding Portugal and (v) to study extreme events of the past, their impacts and the vulnerability of societies to weather during the last 350 years, and compare them with current analogues. With these tasks, we expect to help completing the spatial coverage of past European climate, as the data gap over SW Europe is often mentioned. As the team members come from four different Universities in Portugal (Évora, Lisbon, Oporto and UTAD), we expect to obtain a good spatial representation of documentary evidence. Teams are now progressing in data search activities in archives. An Access database frame was constructed. Some 18th century extreme events have been and are being studied (Barbara storm, Dec.1739, among others). The first workshop took place in Lisbon (October 2012): Prof Brázdil and Dr. Domínguez-Castro (two of our consultants) were keynote speakers. Key-words: Climate reconstruction, Documentary

  18. Analysis of the Type IV Fimbrial-Subunit Gene fimA of Xanthomonas hyacinthi: Application in PCR-Mediated Detection of Yellow Disease in Hyacinths

    PubMed Central

    van Doorn, J.; Hollinger, T. C.; Oudega, B.

    2001-01-01

    A sensitive and specific detection method was developed for Xanthomonas hyacinthi; this method was based on amplification of a subsequence of the type IV fimbrial-subunit gene fimA from strain S148. The fimA gene was amplified by PCR with degenerate DNA primers designed by using the N-terminal and C-terminal amino acid sequences of trypsin fragments of FimA. The nucleotide sequence of fimA was determined and compared with the nucleotide sequences coding for the fimbrial subunits in other type IV fimbria-producing bacteria, such as Xanthomonas campestris pv. vesicatoria, Neisseria gonorrhoeae, and Moraxella bovis. In a PCR internal primers JAAN and JARA, designed by using the nucleotide sequences of the variable central and C-terminal region of fimA, amplified a 226-bp DNA fragment in all X. hyacinthi isolates. This PCR was shown to be pathovar specific, as assessed by testing 71 Xanthomonas pathovars and bacterial isolates belonging to other genera, such as Erwinia and Pseudomonas. Southern hybridization experiments performed with the labelled 226-bp DNA amplicon as a probe suggested that there is only one structural type IV fimbrial-gene cluster in X. hyacinthi. Only two Xanthomonas translucens pathovars cross-reacted weakly in PCR. Primers amplifying a subsequence of the fimA gene of X. campestris pv. vesicatoria (T. Ojanen-Reuhs, N. Kalkkinen, B. Westerlund-Wikström, J. van Doorn, K. Haahtela, E.-L. Nurmiaho-Lassila, K. Wengelink, U. Bonas, and T. K. Korhonen, J. Bacteriol. 179: 1280–1290, 1997) were shown to be pathovar specific, indicating that the fimbrial-subunit sequences are more generally applicable in xanthomonads for detection purposes. Under laboratory conditions, approximately 1,000 CFU of X. hyacinthi per ml could be detected. In inoculated leaves of hyacinths the threshold was 5,000 CFU/ml. The results indicated that infected hyacinths with early symptoms could be successfully screened for X. hyacinthi with PCR. PMID:11157222

  19. Real-Time Seismology in Portugal

    NASA Astrophysics Data System (ADS)

    Custodio, S.; Marreiros, C.; Carvalho, S.; Vales, D.; Lima, V.; Carrilho, F.

    2012-12-01

    Portugal is located next to the plate boundary between Eurasia (Iberia) and Africa (Nubia). The country has been repeatedly affected by some of the largest earthquakes, both onshore and offshore, in the historical European record, including the largest historical European earthquake, the great Lisbon earthquake of 1755 (~M8.5). The Portuguese territory has suffered directly the consequences of strong ground shaking (collapse of buildings, etc) and also some of the most destructive consequences of earthquakes (e.g. tsunamis, fires, etc). However, the rate of tectonic deformation in the Portuguese territory is low (the Eurasian-African plates converge at a rate of ~ 5 mm/yr), which results in long recurrence intervals between earthquakes. This low to moderate rate of seismic activity has two major negative effects: 1) it is difficult to study the regional seismo-tectonics with traditional passive methods; 2) the population is little aware of earthquake risk and unprepared to react in case of disaster. In this scenario, real-time seismology is key to monitoring earthquake crisis in real-time, providing early warnings about potentially destructive events, and assisting in the channeling of recovery efforts in case of disaster. In this paper we will present the real-time algorithms implemented at Instituto de Meteorologia (IM), the institution responsible for seismic monitoring in Portugal. In particular, we will focus on the following aspects: 1) Data collection and real-time transmission to the headquarters. Broadband seismological stations are owned and operated by five different institutions. The last years have witnessed an effort for integration, and presently most data arrives at IM lab in real-time. 2) Earthquake location and local magnitude determination. Data is automatically analyzed in order to obtain a first earthquake hypocenter and ML. While this process is mostly automatic, it still requires the revision by an operator, who is available 24h. 3

  20. The Divergently Transcribed Streptococcus parasanguis Virulence-Associated fimA Operon Encoding an Mn2+-Responsive Metal Transporter and pepO Encoding a Zinc Metallopeptidase Are Not Coordinately Regulated

    PubMed Central

    Oetjen, Joyce; Fives-Taylor, Paula; Froeliger, Eunice H.

    2002-01-01

    The study of how bacteria respond to and obtain divalent metal ions provides insight into the regulation of virulence factors in the host environment. Regulation of metal permease operons in gram-positive bacteria may involve the binding of metal-responsive repressors to palindromic domains in their control regions. The Streptococcus parasanguis fimA operon, which encodes an ATP-binding cassette (ABC) transporter system with sequence homology to the LraI family of metal transporters, possesses a palindromic regulatory region with high homology to that of the Streptococcus gordonii ScaR binding domain. Mapping of the promoter and regulatory regions of fimA and the divergently transcribed pepO gene, which encodes a zinc metalloendopeptidase, indicated that their promoter and regulatory elements overlap. fimA had one transcriptional start site, whereas pepO had three. Analysis of truncated versions of the pepO promoter suggested that all three transcriptional start sites are functional. Analysis of promoter activity under various environmental conditions indicated that the fimA operon promoter and the pepO promoter are not coordinately regulated. The fimA operon is responsive to changes in Mn2+ concentration, but the pepO promoter is not. A S. parasanguis fimA mutant showed a growth deficiency under conditions of limiting Mn2+. This deficiency was not alleviated by compensation with either Mg2+ or Fe3+. Wild-type S. parasanguis could take up Mn2+ and Fe3+, while the fimA mutant showed a marked reduction in this ability. These data suggested that FimA is a component of a metal transporter system capable of transporting both Mn2+ and Fe3+. FimA expression itself was shown to be responsive to Mn2+ concentration, but not to availability of Fe3+ or Mg2+. PMID:12228300

  1. [Approach and Registry of Anaphylaxis in Portugal].

    PubMed

    Mota, Inês; Pereira, Ana Margarida; Pereira, Celso; Tomaz, Elza; Ferreira, Manuel Branco; Sabino, Filipa; Coelho, Anabela; Santos, Anabela; Martins, Henrique; Morais-Almeida, Mário

    2015-01-01

    Anaphylaxis has a growing incidence, especially in children. It represents a medical emergency and its successful therapy depends on early and proper intervention. Intramuscular epinephrine, with dose adjustment according to weight and age, is the drug of choice for anaphylaxis treatment. After resolution of the acute reaction, the patient should be kept under clinical surveillance for 6 to 24 hours, due to possible biphasic reactions. Prescription of an epinephrine auto injectable device should be considered in all patients with diagnosed or suspected anaphylaxis; additionally these patients should always be referred to an Immunoallergy consultation, to perform adequate investigation and management in order to reduce future risk. All anaphylaxis episodes must be recorded in The Portuguese Catalogue of Allergies and other Adverse Reactions (Catálogo Português de Alergias e outras Reações Adversas, CPARA), which represents a fundamental tool to share clinical information within the Health System. The present manuscript intends to disclose the most recent Portuguese guidelines for the diagnosis and treatment of anaphylaxis, making its clinical approach more effective and homogeneous, and to promote the use of The Portuguese Catalogue of Allergies and other Adverse Reactions as an essential tool to register and share information on anaphylaxis in Portugal. PMID:26849768

  2. Spatial and temporal variability of droughts in Portugal

    NASA Astrophysics Data System (ADS)

    Santos, JoãO. Filipe; Pulido-Calvo, Inmaculada; Portela, Maria Manuela

    2010-03-01

    An analysis of droughts in mainland Portugal based on monthly precipitation data, from September 1910 to October 2004, in 144 rain gages distributed uniformly over the country is presented. The drought events were characterized by means of the Standardized Precipitation Index (SPI) applied to different time scales (1, 6, and 12 consecutive months and 6 months from April to September and 12 months from October to September). To assess spatial and temporal patterns of droughts, a principal component analysis (PCA) and K-means clustering (KMC) were applied to the SPI series. In this way, three different and spatially well-defined regions with different temporal evolution of droughts were identified (north, central, and south regions of Portugal). A spectral analysis of the SPI patterns obtained with principal component analysis and clusters analysis, using the fast Fourier transform algorithm (FFT), showed that there is a manifest 3.6-year cycle in the SPI pattern in the south of Portugal and evident 2.4-year and 13.4-year cycles in the north of Portugal. The observation of the drought periods supports the occurrence of more frequent cycles of dry events in the south (droughts from moderate to extreme approximately every 3.6 years) than in the north (droughts from severe to extreme approximately every 13.4 years). These results suggest a much stronger immediate influence of the NAO in the south than in the north of Portugal, although these relations remain a challenging task.

  3. Intervening with Urinary Tract Infections Using Anti-Adhesives Based on the Crystal Structure of the FimH–Oligomannose-3 Complex

    PubMed Central

    Nguyen, Hien; Van Gerven, Nani; Slättegård, Rikard; Hernalsteens, Jean-Pierre; Wyns, Lode; Oscarson, Stefan; De Greve, Henri; Hultgren, Scott; Bouckaert, Julie

    2008-01-01

    Background Escherichia coli strains adhere to the normally sterile human uroepithelium using type 1 pili, that are long, hairy surface organelles exposing a mannose-binding FimH adhesin at the tip. A small percentage of adhered bacteria can successfully invade bladder cells, presumably via pathways mediated by the high-mannosylated uroplakin-Ia and α3β1 integrins found throughout the uroepithelium. Invaded bacteria replicate and mature into dense, biofilm-like inclusions in preparation of fluxing and of infection of neighbouring cells, being the major cause of the troublesome recurrent urinary tract infections. Methodology/Principal Findings We demonstrate that α-d-mannose based inhibitors of FimH not only block bacterial adhesion on uroepithelial cells but also antagonize invasion and biofilm formation. Heptyl α-d-mannose prevents binding of type 1-piliated E. coli to the human bladder cell line 5637 and reduces both adhesion and invasion of the UTI89 cystitis isolate instilled in mouse bladder via catheterization. Heptyl α-d-mannose also specifically inhibited biofilm formation at micromolar concentrations. The structural basis of the great inhibitory potential of alkyl and aryl α-d-mannosides was elucidated in the crystal structure of the FimH receptor-binding domain in complex with oligomannose-3. FimH interacts with Manα1,3Manβ1,4GlcNAcβ1,4GlcNAc in an extended binding site. The interactions along the α1,3 glycosidic bond and the first β1,4 linkage to the chitobiose unit are conserved with those of FimH with butyl α-d-mannose. The strong stacking of the central mannose with the aromatic ring of Tyr48 is congruent with the high affinity found for synthetic inhibitors in which this mannose is substituted for by an aromatic group. Conclusions/Significance The potential of ligand-based design of antagonists of urinary tract infections is ruled by the structural mimicry of natural epitopes and extends into blocking of bacterial invasion, intracellular

  4. Ground Penetrating Radar technique for railway track characterization in Portugal

    NASA Astrophysics Data System (ADS)

    De Chiara, Francesca; Fontul, Simona; Fortunato, Eduardo; D'Andrea, Antonio

    2013-04-01

    Maintenance actions are significant for transport infrastructures but, today, costs have to be necessary limited. A proper quality control since the construction phase is a key factor for a long life cycle and for a good economy policy. For this reason, suitable techniques have to be chosen and non-destructive tests represent an efficient solution, as they allow to evaluate infrastructure characteristics in a continuous or quasi-continuous way, saving time and costs, enabling to make changes if tests results do not comply with the project requirements. Ground Penetrating Radar (GPR) is a quick and effective technique to evaluate infrastructure condition in a continuous manner, replacing or reducing the use of traditional drilling method. GPR application to railways infrastructures, during construction and monitoring phase, is relatively recent. It is based on the measuring of layers thicknesses and detection of structural changes. It also enables the assessment of materials properties that constitute the infrastructure and the evaluation of the different types of defects such as ballast pockets, fouled ballast, poor drainage, subgrade settlement and transitions problems. These deteriorations are generally the causes of vertical deviations in track geometry and they cannot be detected by the common monitoring procedures, namely the measurements of track geometry. Moreover, the development of new GPR systems with higher antenna frequencies, better data acquisition systems, more user friendly software and new algorithms for calculation of materials properties can lead to a regular use of GPR. Therefore, it represents a reliable technique to assess track geometry problems and consequently to improve maintenance planning. In Portugal, rail inspection is performed with Plasser & Theurer EM120 equipment and recently 400 MHz IDS antennas were installed on it. GPR tests were performed on the Portuguese rail network and, as case study in this paper, a renewed track was

  5. Tetracycline antibiotics in hospital and municipal wastewaters: a pilot study in Portugal.

    PubMed

    Pena, A; Paulo, M; Silva, L J G; Seifrtová, M; Lino, C M; Solich, P

    2010-04-01

    This study investigated the occurrence of tetracyclines (TCs), namely minocycline (MIN), TC, and its epimer epitetracycline (ETC), and doxycycline (DC), in four hospital wastewater effluents and its fate in municipal wastewater treatment plants (WWTPs), in Coimbra, Portugal. Analytical determination was carried out by solid-phase extraction followed by liquid chromatography with fluorescence detection. A gradient system with a mobile phase containing oxalic acid 0.02 M and acetonitrile was used. After postcolumn derivatization with magnesium reagent, TCs were detected at lambda(exc) 386 nm and lambda(em) 500 nm. The proposed method allowed good sensitivity, accuracy, and precision. LOQs were 0.5 microg l(-1) for ETC and TC and 15 and 5 microg l(-1) for MIN and DC, respectively. The recovery values ranged between 66.4% and 117.1%, and intraday and interday repeatability was lower than 6.8%. The method was successfully used to determine the presence of the above-mentioned TCs in 24 wastewater composite samples obtained from hospital effluents and from influent and effluent of the WWTP located in Coimbra, Portugal. MIN and TC were found in 41.7% of the samples; ETC and DC were found in 25% and 8.3% of the samples, respectively. The levels found ranged from 6 to 531.7 microg l(-1) in hospital effluents, while its concentrations in WWTP ranged from 95.8 to 915.3 microg l(-1). A seasonal influence in the concentrations found has also been observed, the levels found in samples collected during spring being higher than those observed in samples collected during autumn; however, these are only preliminary results. The WWTP removal rate ranged between 89.5% and 100%. PMID:20213163

  6. Pests of Blueberries on Sao Miguel, Azores, Portugal

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In February and March 2006, two plots of 154 plants of two southern high bush blueberries cultivars, Vaccinium corymbosum cv. ‘Emerald’ and ‘Jewel,’ a rabbiteye cultivar, V. virgatum cv. ‘Spring High,’ were planted in two locations on São Miguel Island, Açores, Portugal. One plot was planted near t...

  7. Making Sense of TV for Children: The Case of Portugal

    ERIC Educational Resources Information Center

    Pereira, Sara; Pinto, Manuel

    2011-01-01

    Empowering children for a critical and judicious use and consumption of media is a main objective of media literacy. This paper aims to examine the range of television programs available for children in Portugal through a comparative analysis of the programming for children broadcast by the four Portuguese terrestrial channels (RTP1, RTP2, SIC and…

  8. Technological Specialisation Courses in Portugal: Description and Suggested Improvements

    ERIC Educational Resources Information Center

    da Costa, Nilza Maria Vilhena Nunes; Simoes, Ana Raquel; Pereira, Giselia Antunes; Pombo, Lucia

    2009-01-01

    This study is a part of the "Post-secondary Vocational Training in Portugal Project: from a description through to suggestions to improve training quality", which ran from 2003 to 2006. This article, which makes use of data obtained from interviews with Directors of Schools which offer technological specialisation courses (CETs) and from…

  9. School Psychology in Portugal: Practitioners' Characteristics and Practices

    ERIC Educational Resources Information Center

    Mendes, Sofia A.; Abreu-Lima, Isabel; Almeida, Leandro S.; Simeonsson, Rune J.

    2014-01-01

    Little empirical evidence is available on the professional characteristics and practices of school psychologists in Portugal. This study surveyed a total of 477 Portuguese school psychologists employed in public (80%) and private schools (20%). Portuguese school psychologists are described with regard to demographic, professional, and educational…

  10. Predicting Child Outcomes from Preschool Quality in Portugal

    ERIC Educational Resources Information Center

    Abreu-Lima, Isabel M. P.; Leal, Teresa B.; Cadima, Joana; Gamelas, Ana Madalena

    2013-01-01

    The main purpose of this study is to analyze whether quality of preschool classrooms relates to 4- and 5-year-old children developmental outcomes. The study was conducted in 60 classrooms in Porto Metropolitan Area, Portugal. Children (N = 215) were evaluated in the literacy, math, and behavior domains. Preschool quality was assessed through…

  11. Emerging Models of Teacher Training: The Case of Portugal

    ERIC Educational Resources Information Center

    Alexandre, Fernando; Ferreira, Manuela; Miranda, Branca

    2004-01-01

    The structural reforms of geography teacher training in Portugal have been justified by the global evolution of the educational system and took into account: (1) the political will to extend the period of compulsory schooling, which now covers the whole of lower secondary education, but will soon cover upper secondary education as well; (2) the…

  12. Open Plan Schools in Portugal: Failure or Innovation?

    ERIC Educational Resources Information Center

    Martinho, Miguel; Freire da Silva, Jose M. R.

    2008-01-01

    Open plan schools have been largely contested in Portugal; many teachers, administrators and even parents consider this model of schooling inappropriate and therefore a failure. Recently however the Escola da Ponte, one of the open plan schools that has survived, was recognised as one of the country's most innovative educational facilities.…

  13. Research Management in Portugal: A Quest for Professional Identity

    ERIC Educational Resources Information Center

    Trindade, Margarida; Agostinho, Marta

    2014-01-01

    Research managers at science-intensive institutions appear as a continuously evolving group of professionals whose identity is somewhat fragmented, even to themselves. In Portugal, specialized research manager roles have rapidly emerged over the last years alongside the development of a small but consolidated scientific system. In order to get an…

  14. Quality of Life in Ageing Societies: Italy, Portugal, and Turkey

    ERIC Educational Resources Information Center

    Arun, Ozgur; Cevik, Aylin Cakiroglu

    2011-01-01

    The aim of this study was to compare and analyze the quality of life of the elderly in three aging countries: Italy, Portugal, and Turkey. This was done by using data provided by the European Quality of Life Survey completed in 2004. By doing so, we could then operationalize the conditions of the elderly in Turkey who have a rapid aging process…

  15. Corporate Blended Learning in Portugal: Current Status and Future Directions

    ERIC Educational Resources Information Center

    Marcal, Julia; Caetano, Antonio

    2010-01-01

    The aim of this study is to characterize the current status of blended learning in Portugal, given that b-learning has grown exponentially in the Portuguese market over recent years. 38 organizations (representing 68% of all institutions certified to provide distance training by the Government Labour Office--DGERT-) participated in this study. The…

  16. Christmas, Natal, Navidad in Cuba, Mexico, and Portugal.

    ERIC Educational Resources Information Center

    Fontes, Maria Joao, Ed.; Fontes, Manuel da Costa, Ed.

    This material is a compilation of junior high school student-developed stories and games about the way Christmas is celebrated in Portugal, Mexico, and Cuba. The material was developed in the classroom by Portuguese, Mexican, and Cuban immigrant students and by American students who do not have a recent immigrant background, all of whom were…

  17. Early Intervention in Portugal: Family Support and Benefits

    ERIC Educational Resources Information Center

    Correia Leite, Carina Sofia; Da Silva Pereira, Ana Paula

    2013-01-01

    This study investigated the support and benefits of early intervention (EI) in families with children with special needs. Data were gathered through a written questionnaire, "Family Benefits Inventory," completed by 126 families with children with special needs supported by EI teams, with ages from six months to six years in Portugal.…

  18. School-Based Sexuality Education in Portugal: Strengths and Weaknesses

    ERIC Educational Resources Information Center

    Rocha, Ana Cristina; Leal, Cláudia; Duarte, Cidália

    2016-01-01

    Portugal, like many other countries, faces obstacles regarding school-based sexuality education. This paper explores Portuguese schools' approaches to implementing sexuality education at a local level, and provides a critical analysis of potential strengths and weaknesses. Documents related to sexuality education in a convenience sample of 89…

  19. Science Policy and the Internationalisation of Research in Portugal

    ERIC Educational Resources Information Center

    Patricio, Maria Teresa

    2010-01-01

    This article will examine Portuguese and European science and higher education policies and their impact on international collaborations. It will do so by looking at two key indicators: international academic mobility and the coauthored international scientific publications. The data show Portugal as an attractive country for foreign students and…

  20. School Closures and Community Revitalisation: The Case of Obidos, Portugal

    ERIC Educational Resources Information Center

    Godinho, Ana Sofia

    2012-01-01

    Rural communities in many countries are faced with the need to restructure their school networks and close some smaller facilities. Obidos, a town in western Portugal, provides a case study of how to meet these challenges by creating new school complexes that offer improved educational opportunities to the teaching staff, students and local…

  1. Hydrogeology framework of the Azores archipelago (Portugal)

    NASA Astrophysics Data System (ADS)

    Cruz, J. V.; Coutinho, R. M.

    2011-12-01

    The archipelago of the Azores is made of nine islands located in the North Atlantic Ocean, spread along a 600 km-long NW-SE strip, approximately 1600 km from Portugal mainland. All the islands are of volcanic origin and only in Santa Maria, the eastern most island of the Azores, are there sedimentary units interbedded in the volcanic succession of basic nature. Groundwater resources in the Azores are essential both as drinking water source and for ecosystem support. The Azores climate can be considered as marine temperate, with an average annual precipitation at the Azores equal to 1930 mm, exceeding by far the average annual actual evapotranspiration (581 mm). From October to March about 75% of the annual precipitation is registered, which also influences recharge distribution along the year. Recharge rates range from 8.5% to 62.1%, with the highest values been observed at Pico, Terceira, Faial, São Miguel and Graciosa islands, especially in areas where the terrain is covered by recent basaltic lava flows and the soil cover is thin, and the groundwater resources estimates point to a total volume of about 1600x106 m3/yr. Despite differences in the volcanic successions observed in the several islands, resulting from volcanic eruptions of various types, groundwater occurrence can be generally described in terms of two main aquifers systems: (1) the basal aquifer system, which corresponds to fresh-water lenses floating on underlying salt water, and (2) perched-water bodies, which are usually drained by springs spread in the volcanic edifices slopes. Specific well capacity ranges between 1.40x10-2 L/sm and 266.67 L/sm, with a median of 32.29 L/sm (Cruz 2001a, 2004). The values exhibit marked variability among the wells from the different islands. The highest specific capacity is observed on Pico and Graciosa, with wells drilled in recent basaltic lava flows, which generally are thin and fractured with frequently interbedded clincker levels. Transmissivity values also

  2. Human case of West Nile neuroinvasive disease in Portugal, summer 2015.

    PubMed

    Zé-Zé, Líbia; Proença, Paula; Osório, Hugo C; Gomes, Salomé; Luz, Teresa; Parreira, Paulo; Fevereiro, Miguel; Alves, Maria João

    2015-01-01

    A case of West Nile virus (WNV) infection was reported in the Algarve region, Portugal, in the first week of September 2015. WNV is known to circulate in Portugal, with occasional reports in horses and birds (2004 to 2011) and very sporadically human cases (in 2004 and in 2010). Here we present the clinical and laboratory aspects related to the first human case of West Nile neuroinvasive disease reported in Portugal. PMID:26535915

  3. Portugal and United States cooperative energy assessment. Volume 3. Reference reports, Part 1

    SciTech Connect

    Not Available

    1981-09-01

    Statistical data on energy production and consumption and supporting information were obtained from US Bureau of Mines records supplemented by additional data obtained in Portugal. Geologic descriptions and analysis of known areas and of areas having possible future potential have been prepared by the US Geological Survey. Portugal lacks sufficient indigenous supplies of organic fuels to meet its energy demands, and so must import large quantities of petroleum and coal. Approximately 80% of Portugal's electric energy is produced by hydroelectric stations; thermal stations produce the other 20%. Portugal has produced no crude oil, natural gas, or condensate; no resources or reserves in these categories are listed for Portugal in the 1976 World Energy Conference report. Until the last year or so (1980), no significant onshore petroleum exploration had been done in Portugal since 1963. Production of coal in Portugal has declined steadily to the present annual yield of about 200,000 metric tons. On the basis of estimates in only three coal fields, resources of coal of all ranks in Portugal total at least 76 million (10/sup 6/) metric tons. Uranium is mined near Viseu and Guarda in the northern part of Portugal; the Nisa mine in east-central Portugal will begin producing uranium ore in 1985 after installation of a processing plant. Portugal produced 95 metric tons of uranium oxide (U/sub 3/O/sub 8/) from ore stocks in each year from 1972 through 1974; production is assumed to have continued at the same rate since then. Geothermal energy has not been developed in mainland Portugal; however, hot springs that may have geothermal energy potential are known in the Minho district in the northwest. Geothermal energy resources exist in the Azores and a program of evaluation and exploration with technical assistance from the USGS is presently in progress there.

  4. Zika virus infections imported from Brazil to Portugal, 2015

    PubMed Central

    Zé-Zé, L.; Prata, M.B.; Teixeira, T.; Marques, N.; Mondragão, A.; Fernandes, R.; Saraiva da Cunha, J.; Alves, M.J.

    2016-01-01

    Zika virus is an emerging arbovirus transmitted by Aedes sp. mosquitoes like the Dengue and Chikungunya viruses. Zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. In 2007, an epidemic in Micronesia initiated the turnover in the epidemiological history of Zika virus and more recently, the potential association with congenital microcephaly cases in Brazil 2015, still under investigation, led the World Health Organization (WHO) to declare a Public Health Emergency of International Concern on February 1, 2016. Here, we present the clinical and laboratory aspects related to the first four imported human cases of Zika virus in Portugal from Brazil, and alert, regarding the high level of traveling between Portugal and Brazil, and the ongoing expansion of this virus in the Americas, for the threat for Zika virus introduction in Europe and the possible introduction to Madeira Island where Aedes aegypti is present. PMID:27134823

  5. Molecular Identification of Borrelia miyamotoi in Ixodes ricinus from Portugal.

    PubMed

    Nunes, Mónica; Parreira, Ricardo; Lopes, Nádia; Maia, Carla; Carreira, Teresa; Sousa, Carmelita; Faria, Sofia; Campino, Lenea; Vieira, M Luísa

    2015-08-01

    Borrelia miyamotoi, a relapsing fever spirochete, has been found recently in Ixodes ricinus ticks; however, little is known about its spatial distribution and potential local impact on human health. A total of 640 ticks (447 nymphs and 193 adults) collected throughout Portugal were analyzed using two nested PCR protocols, one targeting the flagellin gene and the other the internal transcribed space region between the 5S and the 23S rRNA. As a result, B. miyamotoi was detected, for the first time, in one guesting I. ricinus nymph collected in the Lisboa district. In addition, a prevalence of 11% (71/640) for B. burgdorferi sensu lato was obtained. Even though no human relapsing fever cases due to infection by B. miyamotoi have been reported yet in Portugal, surveillance must be improved to provide better insight into the prevalence and distribution of this spirochete in ticks. PMID:26273814

  6. Detection of Dirofilaria repens microfilariae in a dog from Portugal.

    PubMed

    Maia, Carla; Lorentz, Susanne; Cardoso, Luís; Otranto, Domenico; Naucke, Torsten J

    2016-01-01

    Dirofilaria repens causes subcutaneous infection in dogs and cats, and is the main agent of human dirofilariosis in Europe. Detection of D. repens is described in a dog from Portugal, a finding that simultaneously represents the first presumable case of autochthonous infection with the parasite in any animal host species in the country. A mixed D. repens/Dirofilaria immitis infection (20:1 ratio) was found, with an overall density of 1267 microfilariae per millilitre of blood. Morphological features, including morphometry, and acid phosphatase histochemical staining confirmed identity of the two filarioids. Distribution of D. repens in Portugal should be further assessed at the population level both in vertebrate hosts and in vectors. Due to the zoonotic potential of the parasite, preventive measures such as the administration of microfilaricides and insect repellents should be put into practice to protect animals and public health. PMID:26486944

  7. Zika virus infections imported from Brazil to Portugal, 2015.

    PubMed

    Zé-Zé, L; Prata, M B; Teixeira, T; Marques, N; Mondragão, A; Fernandes, R; Saraiva da Cunha, J; Alves, M J

    2016-01-01

    Zika virus is an emerging arbovirus transmitted by Aedes sp. mosquitoes like the Dengue and Chikungunya viruses. Zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. In 2007, an epidemic in Micronesia initiated the turnover in the epidemiological history of Zika virus and more recently, the potential association with congenital microcephaly cases in Brazil 2015, still under investigation, led the World Health Organization (WHO) to declare a Public Health Emergency of International Concern on February 1, 2016. Here, we present the clinical and laboratory aspects related to the first four imported human cases of Zika virus in Portugal from Brazil, and alert, regarding the high level of traveling between Portugal and Brazil, and the ongoing expansion of this virus in the Americas, for the threat for Zika virus introduction in Europe and the possible introduction to Madeira Island where Aedes aegypti is present. PMID:27134823

  8. Epidemiological review of Toxoplasma gondii infection in humans and animals in Portugal.

    PubMed

    Lopes, A P; Dubey, J P; Dardé, M-L; Cardoso, L

    2014-11-01

    Toxoplasmosis is a worldwide zoonosis. However, data from Portugal are limited and a considerable part of the literature is in Portuguese. Currently, the rate of congenital infection in Portugal is unknown, and almost nothing is known of sequelae of congenital toxoplasmosis. There is no recent general population-based serological survey of Toxoplasma gondii in humans in Portugal. In addition, there is little information on genetic characteristics of T. gondii in animals and humans. In the present paper, we review prevalence, clinical spectrum and epidemiology of T. gondii in humans and animals in Portugal. This knowledge should be useful to biologists, public health workers, physicians and veterinarians. PMID:25215422

  9. Asthma in an Urban Population in Portugal: A prevalence study

    PubMed Central

    2011-01-01

    Background The prevalence and incidence of asthma are believed to be increasing but research on the true incidence, prevalence and mortality from asthma has met methodological obstacles since it has been difficult to define and diagnose asthma in epidemiological terms. New and widely accepted diagnostic criteria for asthma present opportunities for progress in this field. Studies conducted in Portugal have estimated the disease prevalence between 3% and 15%. Available epidemiological data present a significant variability due to methodological obstacles. Aim To estimate the true prevalence of asthma by gender and age groups in the population of the area covered by one urban Health Centre in Portugal. Method An observational study was conducted between February and July 2009 at the Horizonte Family Health Unit in Matosinhos, Portugal. A random sample of 590 patients, stratified by age and gender was obtained from the practice database of registered patients. Data was collected using a patient questionnaire based on respiratory symptoms and the physician's best knowledge of the patient's asthma status. The prevalence of asthma was calculated by age and gender. Results Data were obtained from 576 patients (97.6% response rate). The mean age for patients with asthma was 27.0 years (95% CI: 20.95 to 33.16). This was lower than the mean age for non-asthmatics but the difference was not statistically significant. Asthma was diagnosed in 59 persons giving a prevalence of 10.24% (95% CI: 8.16 to 12.32). There was no statistically significant difference in the prevalence of asthma by gender. Conclusion The prevalence of asthma found in the present study was higher than that found in some studies, though lower than that found in other studies. Further studies in other regions of Portugal are required to confirm these findings. PMID:21595928

  10. Syphilis hospitalisations in Portugal over the last decade.

    PubMed

    Sousa-Pinto, B; Freitas, A; Lisboa, C

    2016-02-01

    Although several studies have reported an increase of syphilis incidence over the last decade in Western Europe, information concerning syphilis epidemiology in Portugal remains scarce. Therefore, we sought to characterise acquired syphilis-associated hospitalisations in Portugal according to demographic and clinical data. We used a database containing all hospitalisations that occurred in mainland Portugal public hospitals with discharges between 2000 and 2014. We analysed all hospitalisations associated with ICD-9-CM codes 091-097.x (corresponding to acquired syphilis diagnosis) concerning inpatients' gender, age and comorbidities. The median length of stay and in-hospital mortality rates were also studied. Between 2000 and 2014, there were a total of 8974 syphilis-related hospitalisations in mainland Portugal. The rate of acquired syphilis hospitalisations per 100,000 inhabitants increased by 33 % during the studied period. Syphilis hospitalisation rates increased by 70 % in males and 139 % among patients aged over 55 years. On the other hand, they declined by 10 % in females and 20 % among patients younger than 55 years old. The percentage of syphilis episodes presenting cardiovascular and neuropsychiatric comorbidities increased, while the percentage of syphilis episodes presenting HIV co-infection decreased by 69 %. A fatal outcome was reported in 5 % of episodes; 4.6 % of them had acquired syphilis as the main reason for hospitalisation. This study illustrates that, despite being a preventable infection, syphilis remains a public health problem. The analysis of hospitalisation and administrative data helps to understand syphilis epidemiology and provides a supplement to traditional case notifications. PMID:26581424

  11. Chewing lice (Phthiraptera) from wild birds in southern Portugal.

    PubMed

    Tomás, André; Palma, Ricardo L; Rebelo, Maria Teresa; da Fonseca, Isabel Pereira

    2016-06-01

    This study was carried out to determine chewing louse species of wild birds in the Ria Formosa Natural Park, located in southern Portugal. In addition, the hypothesis that bird age, avian migration and social behaviour have an impact on the louse prevalence was tested. Between September and December of 2013, 122 birds (belonging to 10 orders, 19 families, 31 genera and 35 species) captured in scientific ringing sessions and admitted to the Wildlife Rehabilitation and Investigation Centre of Ria Formosa were examined for lice. Twenty-six (21.3%) birds were found to be infested with at least one chewing louse species. The chewing lice identified include 18 species. Colonial birds (34.9%) and migratory birds (29.5%) had statistically significant higher prevalence than territorial birds (6.8%) and resident birds (13.1%), respectively. This paper records 17 louse species for the first time in southern Portugal: Laemobothrion maximum, Laemobothrion vulturis, Actornithophilus piceus lari, Actornithophilus umbrinus, Austromenopon lutescens, Colpocephalum heterosoma, Colpocephalum turbinatum, Eidmanniella pustulosa, Nosopon casteli, Pectinopygus bassani, Pseudomenopon pilosum, Trinoton femoratum, Trinoton querquedulae, Craspedorrhynchus platystomus, Degeeriella fulva, Falcolipeurus quadripustulatus, Lunaceps schismatus. Also a nymph of the genus Strigiphilus was collected from a Eurasian eagle-owl. These findings contribute to the knowledge of avian chewing lice from important birds areas in Portugal. PMID:26899014

  12. Differentiation of Aurantii Fructus Immaturus from Poniciri Trifoliatae Fructus Immaturus using Flow- injection Mass spectrometric (FIMS) Metabolic Fingerprinting Method Combined with Chemometrics

    PubMed Central

    Zhao, Yang; Chang, Yuan-Shiun; Chen, Pei

    2015-01-01

    A flow-injection mass spectrometric metabolic fingerprinting method in combination with chemometrics was used to differentiate Aurantii Fructus Immaturus from its counterfeit Poniciri Trifoliatae Fructus Immaturus. Flow-injection mass spectrometric (FIMS) fingerprints of 9 Aurantii Fructus Immaturus samples and 12 Poniciri Trifoliatae Fructus Immaturus samples were acquired and analyzed using principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA). The authentic herbs were differentiated from their counterfeits easily. Eight characteristic components which were responsible for the difference between the samples were tentatively identified. Furthermore, three out of the eight components, naringin, hesperidin, and neohesperidin, were quantified. The results are useful to help identify the authenticity of Aurantii Fructus Immaturus. PMID:25622204

  13. Atmospheric circulation classification comparison based on wildfires in Portugal

    NASA Astrophysics Data System (ADS)

    Pereira, M. G.; Trigo, R. M.

    2009-04-01

    Atmospheric circulation classifications are not a simple description of atmospheric states but a tool to understand and interpret the atmospheric processes and to model the relation between atmospheric circulation and surface climate and other related variables (Radan Huth et al., 2008). Classifications were initially developed with weather forecasting purposes, however with the progress in computer processing capability, new and more robust objective methods were developed and applied to large datasets prompting atmospheric circulation classification methods to one of the most important fields in synoptic and statistical climatology. Classification studies have been extensively used in climate change studies (e.g. reconstructed past climates, recent observed changes and future climates), in bioclimatological research (e.g. relating human mortality to climatic factors) and in a wide variety of synoptic climatological applications (e.g. comparison between datasets, air pollution, snow avalanches, wine quality, fish captures and forest fires). Likewise, atmospheric circulation classifications are important for the study of the role of weather in wildfire occurrence in Portugal because the daily synoptic variability is the most important driver of local weather conditions (Pereira et al., 2005). In particular, the objective classification scheme developed by Trigo and DaCamara (2000) to classify the atmospheric circulation affecting Portugal have proved to be quite useful in discriminating the occurrence and development of wildfires as well as the distribution over Portugal of surface climatic variables with impact in wildfire activity such as maximum and minimum temperature and precipitation. This work aims to present: (i) an overview the existing circulation classification for the Iberian Peninsula, and (ii) the results of a comparison study between these atmospheric circulation classifications based on its relation with wildfires and relevant meteorological

  14. Training in the Retail Trade in Portugal. Report for the FORCE Programme. Retail Sector.

    ERIC Educational Resources Information Center

    Cerdeira, Maria Conceicao; And Others

    A study examined training in the retail trade in Portugal. Employment, work, and training patterns in Portugal's retail sector were researched, and case studies of two firms in the supermarket/hypermarket group, one firm in the stationary/cigarette shop sector, and one clothing manufacturer/retailer were conducted. Teams of researchers interviewed…

  15. Developmental Dyslexia: Perspectives on Teacher Training and Learning Disabilities in Portugal

    ERIC Educational Resources Information Center

    Carvalhais, Lenia; da Silva, Carlos Fernandes

    2010-01-01

    The focus of this study was to explore primary teachers' attitudes and perceptions toward their training experiences, instructional practices, and supports received in their schools toward working with students with dyslexia in Portugal. Primary school teachers from the centre of Portugal, working with children between the first and fourth grades,…

  16. Assessing International Product Design and Development Graduate Courses: The MIT-Portugal Program

    ERIC Educational Resources Information Center

    Dori, Yehudit Judy; Silva, Arlindo

    2010-01-01

    The Product Design and Development (PDD) course is part of the graduate curriculum in the Engineering Design and Advanced Manufacturing (EDAM) study in the MIT-Portugal Program. The research participants included about 110 students from MIT, EDAM, and two universities in Portugal, Instituto Superior Técnico-Universidade Técnica de Lisboa (IST) and…

  17. The Financing of Vocational Education and Training in Portugal. Financing Portrait. CEDEFOP Panorama Series.

    ERIC Educational Resources Information Center

    Centeno, Luis Gomes; Sarmento, Ana Leonor

    The financing of vocational education and training (VET) in Portugal was examined. The examination focused on the following topics: (1) the economic, political, legal, and administrative contexts of funding; (2) the funding framework for VET in Portugal; (3) initial vocational training (IVT); (4) continuing vocational training (CVT); and (5)…

  18. Epidemiological review of Toxoplasma gondii infection in humans and animals in Portugal

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Toxoplasmosis is a worldwide zoonosis. However, data from Portugal are limited and a considerable part of the literature is in Portuguese. Currently, the rate of congenital infection in Portugal is unknown, and almost nothing is known of sequelae of congenital toxoplasmosis. There is no general popu...

  19. Differentiating organically and conventionally grown oregano using ultraperformance liquid chromatography mass spectrometry (UPLC-MS), headspace gas chromatography with flame ionization detection (headspace-GC-FID), and flow injection mass spectrum (FIMS) fingerprints combined with multivariate data analysis.

    PubMed

    Gao, Boyan; Qin, Fang; Ding, Tingting; Chen, Yineng; Lu, Weiying; Yu, Liangli Lucy

    2014-08-13

    Ultraperformance liquid chromatography mass spectrometry (UPLC-MS), flow injection mass spectrometry (FIMS), and headspace gas chromatography (headspace-GC) combined with multivariate data analysis techniques were examined and compared in differentiating organically grown oregano from that grown conventionally. It is the first time that headspace-GC fingerprinting technology is reported in differentiating organically and conventionally grown spice samples. The results also indicated that UPLC-MS, FIMS, and headspace-GC-FID fingerprints with OPLS-DA were able to effectively distinguish oreganos under different growing conditions, whereas with PCA, only FIMS fingerprint could differentiate the organically and conventionally grown oregano samples. UPLC fingerprinting provided detailed information about the chemical composition of oregano with a longer analysis time, whereas FIMS finished a sample analysis within 1 min. On the other hand, headspace GC-FID fingerprinting required no sample pretreatment, suggesting its potential as a high-throughput method in distinguishing organically and conventionally grown oregano samples. In addition, chemical components in oregano were identified by their molecular weight using QTOF-MS and headspace-GC-MS. PMID:25050447

  20. Differentiation of leaf and whole-plant samples of di- and tetraploid Gynostemma pentaphyllum (Thunb.) Makino using flow-injection mass spectrometric(FIMS) fingerprinting method combined with chemometric approaches

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the present study, the feasibility and advantages of employing a flow-injection mass spectrometry (FIMS) fingerprinting method combined with chemometric analyses for assessment of di- and tetraploid leaf and whole-plant Gynostemma. pentaphyllum (Thunb.) Makino samples were investigated for the fi...

  1. Differentiation of leaf and whole-plant samples of di- and tetraploid Gynostemma pentaphyllum (Thunb.) Makino using flow-injection mass spectrometric(FIMS) fingerprinting method combined with chemometric approaches

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the present study, the feasibility and advantages of employing a flow-injection mass spectrometry (FIMS) fingerprinting method combined with chemometric analyses for quality assessment of di- and tetraploid leaf and whole-plant Gynostemma. pentaphyllum (Thunb.) Makino samples were investigated fo...

  2. Holocene estuary development in the Algarve Region (Southern Portugal)

    NASA Astrophysics Data System (ADS)

    Schneider, Heike; Höfer, Dana; Trog, Carmen; Hempel, Rita; Daut, Gerhard; Mäusbacher, Roland

    2010-05-01

    Former coastal estuaries in the Algarve region of South Portugal are used for reconstruction coastal evolution since first marine transgression processes at about 8.000 years before. The sediments of these archives allow high resolution analyses of geochemical and palynological signals. Drillings in different lagoons of the Algarve region contain the sequences from the fluvial sediments during the early Holocene, marine transgression facies during the middle Holocene and the marine/fluvial sediment deposits until present. The results of the sedimentological, geochemical and palynological analyses show that each estuary developed differently, depending on the morphology of the paleovalley, environmental conditions and especially the influence of the sea and the formation of barrier systems. The estuaries were flooded between 7500 and 5500 cal a BP by sea level rise and were almost completely filled by sediment by the beginning of the Roman occupation (226 y BC / 2176 cal a BP). A clear change in sedimentological processes is evident in the estuaries between 5500 and 3000 cal a BP and is interpreted as a result of high energy events such as storms or tsunamis (Schneider et al. 2009, Hilbich et al. 2008 ). Palynological as well as archaeological investigations show distinct anthropogenic influences since 3500 cal. BP by increasing values in maquies, cereals and open land communities. References Hilbich, C., Mügler, I., Daut, G., Frenzel, P., van der Borg, K., Mäusbacher, R. (2008): Reconstruction of the depositional history of the former coastal lagoon of Vilamoura (Algarve, Portugal): A sedimentological, microfaunal and geophysical approach.- Journal of Coastal Research 24(2B), 83-91. Schneider, H., Höfer, D., Trog, C., Busch, S., Schneider, M., Baade, J., Daut, G. & R. Mäusbacher (2009): Holocene estuary development in the Algarve Region (Southern Portugal) - A reconstruction of sedimentological and ecological evolution. - Quaternary International (In Press

  3. Determinants of birth weight in Portugal: 1988 to 2011.

    PubMed

    Fuster, Vicente; Santos, Carlota

    2016-01-01

    The objective of this paper is to analyse temporal birth weight variation, its relationship to the frequency of premature births in Portugal, and the influence of native and immigrant mothers' characteristics as well as to determine the possible existence of a pattern of temporal change in birth weight in the Iberian Peninsula as a whole. Individual mother-child data from the Portuguese National Institute of Statistics regarding live births (N = 2,661,542) permitted an analysis, for the first time, of weight at birth in Portugal from a bio-demographic perspective. The results obtained show that from 1988 to 2011 there was a gradual decline in the average weight at birth in Portugal that may be related to shifts in the duration of gestation. An initial rapid decline in the relative frequency of post-term births took place, followed by small variations from 1995 on. Logistic regressions indicated a pattern unaffected by maternal origin or the sex of the newborn. With regard to weeks of gestation, the odds values obtained were < 1 when the reference category was < 28 weeks. For this factor, no significant differences were found in relation to the mother's origin. Portuguese mothers over 35 years were associated with a higher incidence of low birth weight. Regardless of maternal origin, being a newborn of parity 1, and with the mother not in a couple, resulted in unfavourable outcomes with regard to low birth weight. On the other hand, long gestation periods and having secondary or university education constituted a protective factor. PMID:26754741

  4. Drought and vegetation stress monitoring in Portugal using satellite data

    NASA Astrophysics Data System (ADS)

    Gouveia, C.; Trigo, R. M.; Dacamara, C. C.

    2009-02-01

    Remote sensed information on vegetation and soil moisture, namely the Normalised Difference Vegetation Index (NDVI) and the Soil Water Index (SWI), is employed to monitor the spatial extent, severity and persistence of drought episodes over Continental Portugal, from 1999 to 2006. The severity of a given drought episode is assessed by evaluating the cumulative impact over time of drought conditions on vegetation. Special attention is given to the drought episodes that have occurred in the last decade, i.e., 1999, 2002 and particularly the major event of 2005. During both the 1999 and 2005 drought episodes negative anomalies of NDVI are observed over large sectors of Southern Portugal for up to nine months (out of eleven) of the vegetative cycle. On the contrary, the 2002 event was characterized by negative anomalies in the northern half of Portugal and for a shorter period (eight out of eleven months). The impact of soil moisture on vegetation dynamics is evaluated by analyzing monthly anomalies of SWI and by studying the annual cycle of SWI vs. NDVI. While in the case of the drought episode of 1999 the scarcity of water in the soil persisted until spring, in the recent episode of 2005 the deficit in greenness was already apparent at the end of summer. The impact of dry periods on vegetation is clearly observed in both arable land and forest, and it is found that arable land presents a higher sensitivity. From an operational point of view, obtained results reveal the possibility of using the developed methodology to monitor, in quasi real-time, vegetation stress and droughts in Mediterranean ecosystems.

  5. Weather types and the regime of wildfires in Portugal

    NASA Astrophysics Data System (ADS)

    Pereira, M. G.; Trigo, R. M.; Dacamara, C. C.

    2009-04-01

    An objective classification scheme, as developed by Trigo and DaCamara (2000), was applied to classify the daily atmospheric circulation affecting Portugal between 1980 and 2007 into a set of 10 basic weather types (WTs). The classification scheme relies on a set of atmospheric circulation indices, namely southerly flow (SF), westerly flow (WF), total flow (F), southerly shear vorticity (ZS), westerly shear vorticity (ZW) and total vorticity (Z). The weather-typing approach, together with surfacemeteorological variables (e.g. intensity and direction of geostrophic wind, maximum and minimum temperature and precipitation) were then associated to wildfire events as recorded in the official Portuguese fire database consisting of information on each fire occurred in the 18 districts of Continental Portugal within the same period (>450.000 events). The objective of this study is to explore the dependence of wildfire activity on weather and climate and then evaluate the potential of WTs to discriminate among recorded wildfires on what respects to their occurrence and development. Results show that days characterised by surface flow with an eastern component (i.e. NE, E and SE) account for a high percentage of daily burnt area, as opposed to surface westerly flow (NW, W and SW), which represents about a quarter of the total number of days but only accounts for a very low percentage of active fires and of burnt area. Meteorological variables such as minimum and maximum temperatures, that are closely associated to surface wind intensity and direction, also present a good ability to discriminate between the different types of fire events.. Trigo R.M., DaCamara C. (2000) "Circulation Weather Types and their impact on the precipitation regime in Portugal". Int J of Climatology, 20, 1559-1581.

  6. [Fever and dry cough in a construction worker from Portugal].

    PubMed

    Wiesli, P; Flepp, M; Greminger, P

    1997-07-30

    A 33-year-old Portugese worker presented with a one-week history of nonproductive cough and fever. A presumptive diagnosis "viral infection of the respiratory tract" was made. However, because of persisting cough and fever further investigations were necessary, and finally Brucella melitensis was isolated in blood cultures. Three months before admission to the hospital the man was dressing the carcasses of a goat in Portugal and consumpted fresh goats milk cheese. Antibiotic therapy with Rifampicin and Trimethoprim/Sulfamethoxazol over 6 weeks improved the signs and symptoms of the infection. PMID:9340710

  7. Ochratoxin A in Portugal: A Review to Assess Human Exposure

    PubMed Central

    Duarte, Sofia C.; Pena, Angelina; Lino, Celeste M.

    2010-01-01

    In Portugal, the climate, dietary habits, and food contamination levels present the characteristics for higher population susceptibility to ochratoxin A (OTA), one of the known mycotoxins with the greatest public health and agro-economic importance. In this review, following a brief historical insight on OTA research, a summary of the available data on OTA occurrence in food (cereals, bread, wine, meat) and biological fluids (blood, urine) is made. With this data, an estimation of intake is made to ascertain and update the risk exposure estimation of the Portuguese population, in comparison to previous studies and other populations. PMID:22069635

  8. Evolution of upwelling fronts off the coast of Portugal

    NASA Technical Reports Server (NTRS)

    1994-01-01

    We have analyzed the spatial and temporal variability of chlorophyll in the Atlantic Ocean off the Portugal coast using satellite imagery from 1979 to 1986 in conjunction with wind data. The spatial patterns in the imagery show the typical inshore-offshore and east-west gradients, while the temporal patterns show seasonal high winter values and low summer values. In the satellite imagery for the period 1979-1986, stable mesoscale and large scale patterns recurred despite interannual differences in absolute satellite-derived pigment concentrations.

  9. Inhibition and Reversal of Microbial Attachment by an Antibody with Parasteric Activity against the FimH Adhesin of Uropathogenic E. coli

    PubMed Central

    Friend, Della; Jalan, Aachal; Gupta, Shivani; Interlandi, Gianluca; Liu, Yan; Tchesnokova, Veronika; Rodriguez, Victoria B.; Sumida, John P.; Strong, Roland K.; Wu, Xue-Ru; Thomas, Wendy E.; Sokurenko, Evgeni V.

    2015-01-01

    Attachment proteins from the surface of eukaryotic cells, bacteria and viruses are critical receptors in cell adhesion or signaling and are primary targets for the development of vaccines and therapeutic antibodies. It is proposed that the ligand-binding pocket in receptor proteins can shift between inactive and active conformations with weak and strong ligand-binding capability, respectively. Here, using monoclonal antibodies against a vaccine target protein - fimbrial adhesin FimH of uropathogenic Escherichia coli, we demonstrate that unusually strong receptor inhibition can be achieved by antibody that binds within the binding pocket and displaces the ligand in a non-competitive way. The non-competitive antibody binds to a loop that interacts with the ligand in the active conformation of the pocket but is shifted away from ligand in the inactive conformation. We refer to this as a parasteric inhibition, where the inhibitor binds adjacent to the ligand in the binding pocket. We showed that the receptor-blocking mechanism of parasteric antibody differs from that of orthosteric inhibition, where the inhibitor replaces the ligand or allosteric inhibition where the inhibitor binds at a site distant from the ligand, and is very potent in blocking bacterial adhesion, dissolving surface-adherent biofilms and protecting mice from urinary bladder infection. PMID:25974133

  10. Artisanal salt production in Aveiro/Portugal - an ecofriendly process.

    PubMed

    Rodrigues, Carolina M; Bio, Ana; Amat, Francisco; Vieira, Natividade

    2011-01-01

    Solar salinas are man-made systems exploited for the extraction of salt, by solar and wind evaporation of seawater. Salt production achieved by traditional methods is associated with landscapes and environmental and patrimonial values generated throughout history. Since the mid-twentieth century, this activity has been facing a marked decline in Portugal, with most salinas either abandoned or subjected to destruction, making it necessary to find a strategy to reverse this trend.It is, however, possible to generate revenue from salinas at several levels, not merely in terms of good quality salt production, but also by obtaining other products that can be commercialized, or by exploring their potential for tourism, and as research facilities, among others. Furthermore, with an adequate management, biodiversity can be restored to abandoned salinas, which constitute important feeding and breeding grounds for resident and migratory aquatic birds, many of which are protected by European Community Directives.The aims of this manuscript are to present a brief overview on the current state of sea salt exploitation in Portugal and to stress the importance of recovering these salinas for the conservation of this particular environment, for the regional economy, the scientific community and the general public. The Aveiro salina complex is presented in detail, to exemplify salina structure and functioning, as well as current problems and potential solutions for artisanal salinas. PMID:22053788

  11. Portugal's contribution to navigation and discovery of the New World.

    PubMed

    da Silva, M L

    1993-01-01

    Even before the birth of Prince Henry the Navigator (1394) Portugal had displayed a maritime calling due to its 500-mile shore line and numerous natural bays. Inspired by the riches of India he saw during the Portuguese exploration along the coast of West Africa, Prince Henry set out methodically to collect information by bringing together Jews and Moors with geographical knowledge to found his School of Navigation. From 1415 until his death in 1460 he attracted to his school the foremost contemporary scholars in mathematics, astronomy, and cartography along with experts in knowledge of the compass, astrolabe, water currents, and the winds. This concentration of talent yielded the invention of the caravel, the most important navigational advancement of the time, crucial for long voyages across the high seas. Although the rest of Europe busied itself in political and religious wars, for 70 years (1415-1492) Portugal alone pursued the discovery of the Atlantic. "No nation in the 15th century exhibited so great a spirit of maritime enterprise as the Portuguese." PMID:8354483

  12. Artisanal salt production in Aveiro/Portugal - an ecofriendly process

    PubMed Central

    2011-01-01

    Solar salinas are man-made systems exploited for the extraction of salt, by solar and wind evaporation of seawater. Salt production achieved by traditional methods is associated with landscapes and environmental and patrimonial values generated throughout history. Since the mid-twentieth century, this activity has been facing a marked decline in Portugal, with most salinas either abandoned or subjected to destruction, making it necessary to find a strategy to reverse this trend. It is, however, possible to generate revenue from salinas at several levels, not merely in terms of good quality salt production, but also by obtaining other products that can be commercialized, or by exploring their potential for tourism, and as research facilities, among others. Furthermore, with an adequate management, biodiversity can be restored to abandoned salinas, which constitute important feeding and breeding grounds for resident and migratory aquatic birds, many of which are protected by European Community Directives. The aims of this manuscript are to present a brief overview on the current state of sea salt exploitation in Portugal and to stress the importance of recovering these salinas for the conservation of this particular environment, for the regional economy, the scientific community and the general public. The Aveiro salina complex is presented in detail, to exemplify salina structure and functioning, as well as current problems and potential solutions for artisanal salinas. PMID:22053788

  13. Formaldehyde and acetaldehyde emissions from residential wood combustion in Portugal

    NASA Astrophysics Data System (ADS)

    Cerqueira, Mário; Gomes, Luís; Tarelho, Luís; Pio, Casimiro

    2013-06-01

    A series of experiments were conducted to characterize formaldehyde and acetaldehyde emissions from residential combustion of common wood species growing in Portugal. Five types of wood were investigated: maritime pine (Pinus pinaster), eucalyptus (Eucalyptus globulus), cork oak (Quercus suber), holm oak (Quercus rotundifolia) and pyrenean oak (Quercus pyrenaica). Laboratory experiments were performed with a typical wood stove used for domestic heating in Portugal and operating under realistic home conditions. Aldehydes were sampled from diluted combustion flue gas using silica cartridges coated with 2,4-dinitrophenylhydrazine and analyzed by high performance liquid chromatography with diode array detection. The average formaldehyde to acetaldehyde concentration ratio (molar basis) in the stove flue gas was in the range of 2.1-2.9. Among the tested wood types, pyrenean oak produced the highest emissions for both formaldehyde and acetaldehyde: 1772 ± 649 and 1110 ± 454 mg kg-1 biomass burned (dry basis), respectively. By contrast, maritime pine produced the lowest emissions: 653 ± 151 and 371 ± 162 mg kg-1 biomass (dry basis) burned, respectively. Aldehydes were sampled separately during distinct periods of the holm oak wood combustion cycles. Significant variations in the flue gas concentrations were found, with higher values measured during the devolatilization stage than in the flaming and smoldering stages.

  14. Ditched enclosures in Southern Portugal: an Archaeoastronomical view

    NASA Astrophysics Data System (ADS)

    Mejuto, A J.; Valera, A. Carlos; Castaño, J. Gómez; Rodríguez-Caderot, G.; Becker, Helmut

    2015-05-01

    Since the very first ages, human beings have attempted to understand and manage their environment in order to survive. This is the case regarding wildlife, weather cycles and gathering periods. This is also reflected in the areas surrounding sites where individuals live; they changed the landscape with different aims. This type of archaeology has risen very recently - in the last few years - and is usually called Landscape Archaeology. Traditional landscape archaeology has dealt with earth and location related studies; the relationship of ancient peoples with the sky has been disregarded. The archaeoastronomical studies have mitigated this fact. Archaeoastronomy has revealed an important number of archaeological sites; many of them reveal a clear intention of astronomically designed buildings or structures. This implies a planned detailed design and obviously a deep understanding of astronomical knowledge. As examples of these sites a number of megalithic ditched enclosures sited in Portugal will be shown which were studied inside the project "Ditched enclosures plants and Neolithic cosmologies: A landscape, archaeoastronomical and geophysical perspective". The ideological and astronomical aspects inside the architecture of these types of sites will be explained. In this paper we present a new methodology applied in the archaeoastronomical calculations for southern Portugal sites. It includes GIS techniques and the development of an archaeoastronomical layer that can be used to display the computations over cartographic information from the archaeological sites. A Spatial Data Infrastructure is also created in order to expose the results.

  15. The 23 April 1909 Benavente earthquake (Portugal): macroseismic field revision

    NASA Astrophysics Data System (ADS)

    Teves-Costa, Paula; Batlló, Josep

    2011-01-01

    The 23 April 1909 earthquake, with epicentre near Benavente (Portugal), was the largest crustal earthquake in the Iberian Peninsula during the twentieth century ( M w = 6.0). Due to its importance, several studies were developed soon after its occurrence, in Portugal and in Spain. A perusal of the different studies on the macroseismic field of this earthquake showed some discrepancies, in particular on the abnormal patterns of the isoseismal curves in Spain. Besides, a complete list of intensity data points for the event is unavailable at present. Seismic moment, focal mechanism and other earthquake parameters obtained from the instrumental records have been recently reviewed and recalculated. Revision of the macroseismic field of this earthquake poses a unique opportunity to study macroseismic propagation and local effects in central Iberian Peninsula. For this reasons, a search to collect new macroseismic data for this earthquake has been carried out, and a re-evaluation of the whole set has been performed and it is presented here. Special attention is paid to the observed low attenuation of the macroseismic effects, heterogeneous propagation and the distortion introduced by local amplifications. Results of this study indicate, in general, an overestimation of the intensity degrees previously assigned to this earthquake in Spain; also it illustrates how difficult it is to assign an intensity degree to a large town, where local effects play an important role, and confirms the low attenuation of seismic propagation inside the Iberian Peninsula from west and southwest to east and northeast.

  16. Air Liquide builds H{sub 2} plant in Portugal

    SciTech Connect

    1996-06-19

    Air Liquide will spend $18 million to build a naphtha steam reforming unit in Estarreja, Portugal that will produce 3,700 cu meters/hour of hydrogen (H{sub 2}). The new plant will raise Air Liquide`s H{sub 2} capacity at the site to 8,000 cu meters/hour. The company supplies Anilina de Portugal with H{sub 2}. In addition, Air Liquide supplies Dow Chemical with carbon monoxide used in its methylene di-para-phenylene isocyanate plant at the site. Anilina is spending Esc1.8 billion ($11.3 million) to expand aniline capacity from 60,000 m.t./year to 95,000 m.t./year by the end of 1997 and nitrobenzene from 100,000 m.t./year to 170,000 m.t./year. This year Dow will buy more than 50,000 m.t./year of aniline from the Portuguese firm for its MDI production.

  17. Trichinella britovi in a red fox (Vulpes vulpes) from Portugal.

    PubMed

    Lopes, Ana Patrícia; Vila-Viçosa, Maria João; Coutinho, Teresa; Cardoso, Luís; Gottstein, Bruno; Müller, Norbert; Cortes, Helder C E

    2015-06-15

    Trichinellosis is one of the most important foodborne parasitic zoonoses, caused by nematodes of the genus Trichinella. Pigs and other domestic and wild animals, including red foxes (Vulpes vulpes), are sources of Trichinella infection for human beings. Trichinella britovi is the major agent of infection in sylvatic animals and the most important species circulating in the European wildlife. The present study aimed at assessing Trichinella spp. infection in red foxes from the North of Portugal. Forty-seven carcasses of wild red foxes shot during the official hunting season or killed in road accidents were obtained between November 2008 and March 2010. In order to identify the presence of Trichinella spp. larvae in red foxes, an individual artificial digestion was performed using approximately 30 g of muscle samples. Larvae of Trichinella spp. were detected in one (2.1%) out of the 47 assessed foxes. After a multiplex polymerase chain reaction analysis, T. britovi was molecularly identified as the infecting species. The recognition of T. britovi in a red fox confirms that a sylvatic cycle is present in the North of Portugal and that the local prevalence of Trichinella infection in wildlife must not be ignored due to its underlying zoonotic risks. PMID:25934252

  18. Empirical rainfall thresholds for landslide occurrence in Portugal

    NASA Astrophysics Data System (ADS)

    Zêzere, José Luis; Vaz, Teresa; Pereira, Susana; Oliveira, Sérgio C.; Marques, Rui; Garcia, Ricardo A. C.

    2015-04-01

    Rainfall is the most important physical process responsible for the landslide triggering in Portugal. Following the work of Zêzere et al. (2014), we present the state of the art concerning the proposition of empirical rainfall thresholds in Portugal for different types of landslides observed in different zones of the country: the Lisbon region, the Douro Valley and the NW Mountains, and the Povoação Municipality in São Miguel Island (Azores). The empirical thresholds applied in Portugal are based on the identification of 120 landslide events and include (i) the computation of antecedent rainfall threshold defined by linear regression, (ii) the normalization of rainfall by the mean annual precipitation, (iii) the definition of combined rainfall thresholds, which integrates the rainfall event and the antecedent rainfall for different time periods, and (iv) the definition of lower limit and upper limit rainfall thresholds. The intensity-duration (ID) threshold is the empirical rainfall threshold more used worldwide. In mainland Portugal, the highest ID rainfall threshold is registered in the NW Mountains, which is the rainiest zone of the country. The Lisbon Region typically receives less rain per year and the corresponding ID threshold is lower than that obtained in the north part of the country. The Povoação study area evidence a contrasting situation, which is associated to the highest value of the negative exponential of the threshold (-0.66). As a consequence, for short duration (< 10 h) this threshold is only exceeded in the NW Mountains, while for long durations (>1,000 h) it is below the remaining thresholds. The normalization of the ID threshold by the mean annual precipitation (MAP) has showed that, in relative terms: (i) the ID threshold is highest in Lisbon Region for duration less than 50 h; (ii) in the north of the country, the ID threshold is more exigent in the Douro Valley than in the NW Mountains and (iii) the ID threshold in Povoa

  19. The Non-Fimbriate Phenotype Is Predominant among Salmonella enterica Serovar Choleraesuis from Swine and Those Non-Fimbriate Strains Possess Distinct Amino Acid Variations in FimH

    PubMed Central

    Lee, Chien-An; Yeh, Kuang-Sheng

    2016-01-01

    Although most Salmonella serovars are able to infect a range of animal hosts, some have acquired the ability to cause systemic infections of specific hosts. For example, Salmonella enterica serovar Choleraesuis is primarily associated with systemic infection in swine. Adherence to host epithelial cells is considered a prerequisite for initial infection, and fimbrial appendages on the outer membrane of the bacteria are implicated in this process. Although type 1 fimbriae encoded by the fim gene cluster are commonly found in Salmonella serovars, it is not known whether S. Choleraesuis produces this fimbrial type and if and how fimbriae are involved in pathogenesis. In the present study, we demonstrated that only four out of 120 S. Choleraesuis isolates from pigs with salmonellosis produced type 1 fimbriae as assayed by the yeast agglutination test and electron microscopy. One of the 116 non-type 1 fimbria-producing isolates was transformed with plasmids carrying different fim genes from S. Typhimurium LB5010, a type 1 fimbria-producing strain. Our results indicate that non-type 1 fimbria-producing S. Choleraesuis required only an intact fimH to regain the ability to produce fimbrial appendages. Sequence comparison revealed six amino acid variations between the FimH of the non-type 1 fimbria-producing S. Choleraesuis isolates and those of the type 1 fimbria-producing S. Choleraesuis isolates. S. Choleraesuis that produced type 1 fimbriae contained FimH with an amino acid sequence identical to that of S. Typhimurium LB5010. Site-directed mutagenesis leading to the replacement of the non-conserved residues revealed that a change from glycine to valine at position of 63 (G63V) resulted in a non-type 1 fimbria-producing S. Choleraesuis being able to express type 1 fimbriae on its outer membrane. It is possible that this particular amino acid change prevents this polypeptide from proper interaction with other Fim subunits required for assembly of an intact type 1 fimbrial

  20. Proteins with molecular masses of 50 and 80 kilodaltons encoded by genes downstream from the fimbrilin gene (fimA) are components associated with fimbriae in the oral anaerobe Porphyromonas gingivalis.

    PubMed Central

    Yoshimura, F; Takahashi, Y; Hibi, E; Takasawa, T; Kato, H; Dickinson, D P

    1993-01-01

    Flanking DNA regions of the fimbrilin gene (designated fimA), which encodes the major subunit protein of Porphyromonas (Bacteroides) gingivalis fimbriae, were cloned in several manners from the P. gingivalis chromosome into Escherichia coli by screening with probes derived from a 2.5-kb SacI DNA fragment previously cloned. A total of 10.4 kb of DNA fragments from the P. gingivalis genome was cloned in the pUC plasmid. Expression of the fimA gene and possible flanking genes in the fragments cloned was examined in a pUC plasmid vector system and in a bacteriophage T7 RNA polymerase-promoter expression vector system. The results show that in the pUC plasmid system, a 45-kDa protein, a product of fimA, was only poorly expressed as a precursor of the fimbrilin protein (FimA) and could be detected from cell extracts in Western blotting (immunoblotting) analysis as a sharp band but not in colony immunoblotting analysis. On the other hand, in the T7 RNA polymerase-promoter system, the product of fimA and products of the possible flanking genes responsible for fimbriation were overproduced as thick bands of the 45-kDa protein and as 63-, 50-, and 80-kDa proteins, respectively, in stained electrophoresis gels. All of the recombinant proteins were insoluble and seemed to be expressed as precursors with leader peptides. The 63-kDa, 45-k*Da (a truncated protein of the 50-kDa protein), and 80-kDa proteins were purified after solubilization with sodium dodecyl sulfate. N-terminal amino acid sequences of the 45-k*Da and 80-kDa proteins were analyzed up to the first 35 residues with a gas-phase sequencer. Monospecific antibodies directed to the recombinant proteins, i.e., the 63-kDa, 45-k*Da, and 80-kDa proteins, were raised in rabbits. By using the antibodies, localization of their matured proteins in P. gingivalis was investigated by Western blotting analysis. Immunoblotting analysis suggests that at least the 50- and 80-kDa proteins, encoded by genes downstream from the fim

  1. Anisotropic Tomography of Portugal (West Iberia) from ambient seismic noise

    NASA Astrophysics Data System (ADS)

    Silveira, Graça; Stutzmann, Éléonore; Schimmel, Martin; Dias, Nuno; Kiselev, Sergey; Custódio, Susana; Dundar, Suleyman

    2016-04-01

    Located on the western Iberian Peninsula, Portugal constitutes a key area for accretionary terrane and basin research, providing the best opportunity to probe a crustal formation shaped by the Paleozoic Variscan orogeny followed by the Mesozoic-Cenozoic extensions. The geology of Portugal documents a protracted history from Paleozoic basement formation to the Mesozoic opening of the North Atlantic Ocean. The inheritance of such complex geologic history is yet to be fully determined, playing an important role in the current geodynamic framework influencing, for example, the observed regional seismicity. The physical properties of its crust have largely remained undetermined so far, with unevenly distributed knowledge on the spatial distributions of a detailed crustal structure. Also, the deep seismic reflection/refraction surveys conducted in Western Iberia do not provide a clear picture of the regional characteristics of the crust. Using Seismic Broad Band observations from a dense temporary deployment, conducted between 2010 and 2012 in the scope of the WILAS project and covering the entire Portuguese mainland, we computed a 3D anisotropic model from ambient seismic noise. The dispersion measurements were computed for each station pair using empirical Green's functions generated by cross-correlating one-day-length seismic ambient-noise records. After dispersion analysis, group velocity measurements were regionalized to obtain 2D anisotropic tomographic images. Afterwards, the dispersion curves, extracted from each cell of the 2D group velocity maps, were inverted as a function of depth to obtain a 3D shear wave anisotropic model, using a bayesian approach. A simulated annealing method, in which the number of splines that describes the model, is adapted within the inversion. The models are jointly interpreted with the models gathered from Ps receiver functions as well as with the regional seismicity, enabling to obtain a more detailed picture of the crustal

  2. Multivariate cluster analysis of forest fire events in Portugal

    NASA Astrophysics Data System (ADS)

    Tonini, Marj; Pereira, Mario; Vega Orozco, Carmen; Parente, Joana

    2015-04-01

    Portugal is one of the major fire-prone European countries, mainly due to its favourable climatic, topographic and vegetation conditions. Compared to the other Mediterranean countries, the number of events registered here from 1980 up to nowadays is the highest one; likewise, with respect to the burnt area, Portugal is the third most affected country. Portuguese mapped burnt areas are available from the website of the Institute for the Conservation of Nature and Forests (ICNF). This official geodatabase is the result of satellite measurements starting from the year 1990. The spatial information, delivered in shapefile format, provides a detailed description of the shape and the size of area burnt by each fire, while the date/time information relate to the ignition fire is restricted to the year of occurrence. In terms of a statistical formalism wildfires can be associated to a stochastic point process, where events are analysed as a set of geographical coordinates corresponding, for example, to the centroid of each burnt area. The spatio/temporal pattern of stochastic point processes, including the cluster analysis, is a basic procedure to discover predisposing factorsas well as for prevention and forecasting purposes. These kinds of studies are primarily focused on investigating the spatial cluster behaviour of environmental data sequences and/or mapping their distribution at different times. To include both the two dimensions (space and time) a comprehensive spatio-temporal analysis is needful. In the present study authors attempt to verify if, in the case of wildfires in Portugal, space and time act independently or if, conversely, neighbouring events are also closer in time. We present an application of the spatio-temporal K-function to a long dataset (1990-2012) of mapped burnt areas. Moreover, the multivariate K-function allowed checking for an eventual different distribution between small and large fires. The final objective is to elaborate a 3D

  3. Integration of care systems in Portugal: anatomy of recent reforms

    PubMed Central

    Santana, Silvina; Szczygiel, Nina; Redondo, Patrícia

    2014-01-01

    Background Integrated care is increasingly present in the agenda of policy-makers, health professionals and researchers as a way to improve care services in relation to access, quality, user satisfaction and efficiency. These are overarching objectives of most sectoral reforms. However, health care and social care services and systems are more and more dependent on the performance of each other, imposing the logic of network. Demographic, epidemiologic and cultural changes result in pressure to increase efficiency and efficacy of services and organisations in both sectors and that is why integrated care has become so relevant in the last years. Methods We first used concept maps to organise and systematise information that we had gathered through deep literature review in order to set a framework where to base the subsequent work. Then, we interviewed informants at several levels of the health and social care systems and we built a list of major recent reforms addressing integrated care in Portugal. In a third step, we conducted two independent focus groups where those reforms were discussed and evaluated within the context of the concepts and frameworks identified from the literature. Results were confronted and reconciled, giving place to a list of requisites and guidelines that oriented further search for documentation on those reforms. Results Several important health reforms are in course in primary and hospital care in Portugal, while a so-called third level of care has been introduced with the launch of the National Network of Long-Term Integrated Care (RNCCI – Rede Nacional de Cuidados Continuados Integrados). The social care sector has itself been a subject of alternative models springing from opposite political orientations. All these changes are having repercussions on the way the systems work with each other as they are leading to ongoing and ill-evaluated reformulations on the way they are governed, financed, structured and operated. Conclusions Care

  4. The Ançã limestones, Coimbra, Portugal

    NASA Astrophysics Data System (ADS)

    Quinta-Ferreira, Mário; Gil Catarino, Lídia; Delgado Rodrigues, José

    2016-04-01

    Ançã is located in the Lusitanian Basin (western Meso-Cenozoic sedimentary basin), in the municipality of Cantanhede, close to Coimbra, Portugal. This constitutes the northernmost Dogger (Bajocian) limestone sequence in Portugal. The use of the Ançã limestones is documented since the Roman occupation of the Iberian Peninsula. It was used for the construction of houses, palaces, churches, fine sculptures, carving, paving and for the production of lime. These limestones vary from white and very soft varieties, with very high porosity used for sculpture and carving to white and hard varieties used for masonry and as aggregates and to white to bluish with low porosity and high strength varieties, mainly used for paving. The softer and whiter variety is worldwide known as Ançã Stone (Pedra de Ançã) exhibiting a porosity of 26-29 %. It became famous after being largely used by Coimbra most famous Renaissance sculptors like João de Ruão and Nicolau de Chanterenne. The Pedra de Ançã was used mainly in the region of Coimbra, but also in several other places in Portugal, in Santiago de Compostela, Spain, and Brazil. Some examples of heritage in Coimbra using the Pedra de Ançã are the renaissance portal of the Saint Cross Church, the tombs of the first two Portuguese kings located in this church, the altar of the Saint Cross Church or of the Old Cathedral, or in sculptures at the University of Coimbra. It is quite prone to deteriorate when exposed to atmospheric agents and to soluble salts, mainly due to its high porosity. Deteriorated surfaces needing treatment constitute difficult conservation problems, especially when consolidation and protection treatments are required. The less porous varieties of the Ançã limestones (< 20 % porosity) were mainly used for masonry, paving and production of lime. The royal Palace of Buçaco is a remarkable masonry building constructed at the end of the XIX century with the less porous varieties of the Ançã limestones

  5. Will new gender policies stop the decrease of women physicists in Portugal?

    NASA Astrophysics Data System (ADS)

    Rosa, Carla Carmelo; Peña, Maria Teresa; Saavedra, Luisa; Providência, Constança

    2013-03-01

    The present context of women physicists in Portugal is discussed, updating our report for the 2002 IUPAP International Conference on Women in Physics, in which the 30 years prior to 2000 were analyzed.

  6. Assessing fire risk in Portugal during the summer fire season

    NASA Astrophysics Data System (ADS)

    Dacamara, C. C.; Pereira, M. G.; Trigo, R. M.

    2009-04-01

    Since 1998, Instituto de Meteorologia, the Portuguese Weather Service has relied on the Canadian Fire Weather Index (FWI) System (van Wagner, 1987) to produce daily forecasts of fire risk. The FWI System consists of six components that account for the effects of fuel moisture and wind on fire behavior. The first three components, i.e. the Fine Fuel Moisture Code (FFMC), the Duff Moisture Code (DMC) and the Drought Code (DC) respectively rate the average moisture content of surface litter, decomposing litter, and organic (humus) layers of the soil. Wind effects are then added to FFMC leading to the Initial Spread Index (ISI) that rates fire spread. The remaining two fuel moisture codes (DMC and DC) are in turn combined to produce the Buildup Index (BUI) that is a rating of the total amount of fuel available for combustion. BUI is finally combined with ISI to produce the Fire Weather Index (FWI) that represents the rate of fire intensity. Classes of fire danger and levels of preparedness are commonly defined on an empirical way for a given region by calibrating the FWI System against wildfire activity as defined by the recorded number of events and by the observed burned area over a given period of time (Bovio and Camia, 1998). It is also a well established fact that distributions of burned areas are heavily skewed to the right and tend to follow distributions of the exponential-type (Cumming, 2001). Based on the described context, a new procedure is presented for calibrating the FWI System during the summer fire season in Portugal. Two datasets were used covering a 28-year period (1980-2007); i) the official Portuguese wildfire database which contains detailed information on fire events occurred in the 18 districts of Continental Portugal and ii) daily values of the six components of the FWI System as derived from reanalyses (Uppala et al., 2005) of the European Centre for Medium-Range Weather Forecasts (ECMWF). Calibration of the FWI System is then performed in two

  7. Holocene morphologic changes offshore the Quarteira region, Algarve, south Portugal

    NASA Astrophysics Data System (ADS)

    Noiva, João.; Duarte, Henrique; Terrinha, Pedro; Gonçalves, Daniela

    2010-05-01

    A 160 km long SPARKER single channel seismic reflection survey was acquired in November 2008 on the shallow continental shelf of south Portugal to investigate the recent tectonics of the S.Marcos - Quarteira Fault (SMQF) and of the Carcavai Fault. The NW-SE trending S.Marcos - Quarteira Fault (SMQF) can be traced on the onshore-offshore region of south Portugal for an extension of 160 km. It is a Variscan (Hercynian) orogenic thrust that was inverted as an extensional fault during the Mesozoic rifting in south Portugal, during which it played a major role in the segmentation of the Algarve Basin. The SMQF was re-inverted as a transpressive fault during the Paleogene-Miocene compression and folding and thrusting of Pleistocene terrigenous strata attest for its present day tectonic activity. The Carcavai fault trends NE-SW, intersects the SMQF. The Carcavai fault also played the role of rift fault during the Mesozoic and was inverted as a thrust during the Cenozoic compression. The mapping of both SMQ and Carcavai Faults offshore south Portugal attests for a complex interaction between the two in what concerns the Holocene and tectonics and paleogeography. The Carcavai Fault accommodates most of the Pliocene-Quaternary compression on its hanging-wall, whilst the SMQF takes up the deformation on the foot-wall of the Carcavai Fault. In what refers the early Holocene paleogeography, the interpretation of the seismic profiles shows the existence of two sedimentary bodies of possible Holocene age separated by a peninsula that consists of Pliocene-Pleistocene prograding clastics that make up the present day coastal cliff. This peninsula is parallel to the present day coastline, which is also parallel to the SMQF. The two bodies have different seismic facies. The offshore Holocene displays fairly continuous reflections, whilst the internal Holocene is made up of more discontinuous reflections. The isobaths map of the base of the external Holocene unit shows a smooth gently

  8. Conservation and sustainability in freshwater ecosystems in Tavira (Portugal)

    NASA Astrophysics Data System (ADS)

    Gonçalves, Esmeralda; Fonseca, José; Lopes, Luís; João Costa, Maria; Cunha, Miguel

    2013-04-01

    This interdisciplinary project carried out with 8th and 9th grade students involved five teachers from three different subjects (Physics and Chemistry, Natural Science and Geography). Framed in the Water Framework Directive, it aimed at verifying the ecological quality of water in two rivers in the municipality of Tavira (South Portugal). The development of this project has been structured in accordance with the following objectives: evaluate the quality of freshwater ecosystems through the existence of certain living organisms; present proposals on ways to preserve water resources in a sustainable perspective; sensitize students to the importance of their participation in collective action by volunteering for ecological protection. This is an innovative educational experience that allowed students an integrated approach to content and procedures applied to real problems in their local environment.

  9. Elemental composition of four farmed fish produced in Portugal.

    PubMed

    Lourenço, Helena M; Afonso, Cláudia; Anacleto, Patrícia; Martins, Maria F; Nunes, Maria L; Lino, Ana R

    2012-11-01

    Farmed gilthead sea bream (Sparus aurata), European sea bass (Dicentrarchus labrax), rainbow trout (Oncorhynchus mykiss) and turbot (Psetta maxima) produced in Portugal were analysed in order to characterize their elemental composition. Atomic absorption (flame and cold vapour) and molecular absorption spectrometry techniques were used to determine all the studied elements. Similar patterns of macro, trace and ultra trace elements were observed for all fish species. The main elements were potassium (K), sodium (Na), phosphorus (P), magnesium (Mg) and calcium (Ca), followed by zinc (Zn), iron (Fe), copper (Cu), chromium (Cr), manganese (Mn) and nickel (Ni). Cadmium (Cd), mercury (Hg) and lead (Pb) concentrations, obtained in this study, allow concluding that these species do not present a hazard for human consumption. In addition, they contain almost all essential elements at concentrations sufficient to suit the dietary reference intake. Nevertheless, P. maxima nutritious trace element content is relatively low compared with the other three species. PMID:22574998

  10. Comparing Consumer Resistance to Mobile Banking in Finland and Portugal

    NASA Astrophysics Data System (ADS)

    Laukkanen, Tommi; Cruz, Pedro

    This study compares two distinct European countries namely Finland and Portugal in terms of consumer resistance to mobile banking services. We explored how the five adoption barriers namely usage, value, risk, tradition and image, derived from the earlier literature, differ between these two countries. Using an Internet questionnaire a total of 3.597 usable responses was collected. A confirmatory factor analysis was implemented with SEM to build the constructs’ latent score levels. Using non-parametric difference tests we concluded that the resistance is significantly lower among the Portuguese online bank customers in terms of four out of the five barriers. The results can be used for a better understanding and enhancement of adoption of this specific case of mobile communication.

  11. [Epidemiological study of lung cancer in Portugal (2000/2002)].

    PubMed

    Parente, Bárbara; Queiroga, H; Teixeira, E; Sotto-Mayor, R; Barata, F; Sousa, A; Melo, M J; João, F; Neveda, R; Cunha, J; Fernandes, A; Manuel, M; Cardoso, T; Ferreira, L; Nogueira, F; Duarte, J; Semedo, E; Brito, U; Pimentel, F; Barros, S; Costa, F; Almodôvar, T; Araújo, A

    2007-01-01

    Lung cancer is the most common form of cancer death in the world. Five-year survival is about 15%, without any change to this picture envisaged. It is the 3rd most prevalent type of cancer in Portugal and the primary cause of cancer death. 85% of lung cancer cases are attributable to smoking. One study performed in Portugal for 3 years (2000/2002) by the Lung Oncology Work Committee of the Portuguese Society of Pulmonology in 22 Hospitals showed that of a total of 4396 patients with lung cancer, 81.8% were male and 18.2% were female, with a mean age of 64.49 +/- 11.28 years. About 70% of patients were smokers or former smokers, with 50.3% of patients presenting with performance status (Zubrod) 1. Histologically, 37.5% were adenocarcinoma, followed by squamous carcinoma in 30.5% of cases, and small cell lung cancer in 12.5%; neuroendocrine carcinoma presented in 1.4% of cases; non small cell lung cancer in 10.5%; mixed carcinoma in 0.7%; large cell carcinoma in 2.3%; and others/not specified in 4.6% of cases. Staging (known in 4097 patients), showed 113 patients in stage IA (2.8%)and 250 patients in stage IB (6.1%); only 0.8% in stage IIA and 4.5% in stage IIB; 9.1% in stage IIIA and 29.9% in stage IIIB; 46.9% were already in stage IV by the time of diagnosis. The first therapeutic option was known in 3855 patients. Surgery was performed in 8.2% and 21.8% of cases were treated with combined therapies (surgery and chemotherapy or radiotherapy, or combination of chemotherapy and radiotherapy); chemotherapy alone was first choice in 43.7% of patients and in 20.3% only best support therapy was chosen. PMID:17571453

  12. Influence of meteorological conditions on RSV infection in Portugal

    NASA Astrophysics Data System (ADS)

    Oliveira-Santos, M.; Santos, J. A.; Soares, J.; Dias, A.; Quaresma, M.

    2016-04-01

    Acute viral bronchiolitis is a common cause for infant hospital admissions. Of all etiological agents, respiratory syncytial virus (RSV) is commonly the most frequent. The present study assesses relationships between atmospheric factors and RSV infections in under 3-year-old patients admitted to the Inpatient Paediatric Service of Vila Real (North of Portugal). For this purpose, (1) clinical files of children admitted with a diagnosis of acute bronchiolitis from September 2005 to December 2015 (>10 years) were scrutinised and (2) local daily temperature/precipitation series, as well as six weather types controlling meteorological conditions in Portugal, were used. Fifty-five percent of all 770 admitted children were effectively infected with a given virus, whilst 48 % (367) were RSV+, i.e. 87 % of virus-infected children were RSV+. The bulk of incidence is verified in the first year of age (82 %, 302), slightly higher in males. RSV outbreaks are typically from December to March, but important inter-annual variability is found in both magnitude and shape. Although no clear connections were found between monthly temperatures/precipitation and RSV outbreaks apart from seasonality, a linkage to wintertime cold spells is apparent on a daily basis. Anomalously low minimum temperatures from the day of admittance back to 10 days before are observed. This relationship is supported by anomalously high occurrences of the E and AA weather types over the same period, which usually trigger dry and cold weather. These findings highlight some predictability in the RSV occurrences, revealing potential for modelling and risk assessments.

  13. Tsunami flooding along Tagus estuary, Portugal, the 1531 event

    NASA Astrophysics Data System (ADS)

    Baptista, M. A.; Miranda, J. M.; Batlo, J.; Ferreira, H.

    2012-04-01

    TSUNAMI FLOODING ALONG TAGUS ESTUARY (PORTUGAL), THE 1531 EVENT The city of Lisbon one of the main towns in Europe between the XVI and XVIII centuries was severely damaged by two strong earthquakes: 1531-01-26 and 1755-11-01 and the companion tsunamis. In this study we present a re-evaluation of the data available for this event. The 26 January 1531 earthquake occurred between 4 and 5 am and was felt mainly in Lisbon and surroundings dwellings along the Tagus river estuary. The shock heavily destroyed Lisbon downtown causing approximately 1000 casualties Two foreshocks preceded the event: on the 2nd and the 7th January 1531, respectively. The maximum MSK intensity is IX, making it one of the most disastrous earthquakes in the recent history of Portugal. The historical descriptions clearly describe the observation of high waves and the ships touching the riverbed. Although, the difference between tsunamis and storms is sometimes unclear in some historical documents, in this case, the occurrence of the of the earthquake definitively states clearly excludes the hypothesis of a storm. Moreover, the king's chronicle clearly states the observation of high waves and the lack of wind. Other reports consistent with the occurrence of a tsunami are the observation of strong fluxes and refluxes in the river the division of islands into smaller ones and the observation of the riverbed. In this study we present a re-appraisal of the historical information available, a new isoseismal map and the relocation of the epicentre. Finally, we present a tsunami simulation and propagation along a section of 70 km along Tagus estuary compatible with the earthquake data and the historical accounts.

  14. Parasite communities in stray cat populations from Lisbon, Portugal.

    PubMed

    Waap, H; Gomes, J; Nunes, T

    2014-12-01

    Stray cats live in high-density colonies in urban areas and pose a health hazard to household cats and humans. In Portugal, information on the parasitic fauna of stray cats is limited and relies mostly on results from faecal analysis. The present survey aimed to determine the prevalence, diversity and intensity of parasites in stray cats from the urban area of Lisbon by means of parasitological necropsy. Internal organs were collected from 162 cats captured in different areas of the city and systematically subjected to parasitological dissection. Helminths were identified by macro- and microscopic examination and protozoa by faecal floatation and sedimentation techniques. The overall prevalence of parasites was 90.7% (95% confidence interval (CI): 85.3-94.6%). A total of 12 parasite species was recorded: Cystoisospora felis (14.2%), Cystoisospora rivolta (46.3%), Sarcocystis sp. (1.2%), Ancylostoma tubaeforme (19.1%), Toxocara cati (38.3%), Ollulanus tricuspis (30.9%), Aelurostrongylus abstrusus (12.4%), Eucoleus aerophilus (0.6%), Taenia taeniaeformis (3.1%), Dipylidium caninum (53.1%), Joyeuxiella pasqualei (15.4%) and Diplopylidium nölleri (3.7%). Overall mean species richness was 2.36 ±  1.52. Helminth mean intensity was highest for O. tricuspis (285.8), followed by D. caninum (42.4), J. pasqualei (14.4), A. tubaeforme (8.1) and T. cati (5.9). The prevalence and variety of parasites found in our sampling are substantially higher than the numbers previously reported in Portugal. Some of the parasites, including T. cati and A. tubaeforme, are zoonotic, which emphasizes the need for parasite control strategies based on demographic containment of stray cat populations in urban areas to promote public health protection. PMID:23719370

  15. Suicides in the Centre of Portugal: seven years analysis.

    PubMed

    Dias, Daniel; Mendonça, M Cristina; Real, Francisco Corte; Vieira, Duarte Nuno; Teixeira, Helena M

    2014-01-01

    As one of the more specific and distinctive problems of human beings, suicide has been investigated with increasing attention all over the world. Several risk factors have been described as well as limitations arising from their study. The World Health Organization estimates that this scourge affects one million people annually, which corresponds to one death every 40s worldwide. According to recent studies, Portugal, despite the good rates (10 suicide deaths per 100,000 inhabitants), had shown an increasing trend among younger people. This work aims to characterize the evolution of the suicidal profile autopsied at the Forensic Pathology Department of the Centre Branch of the National Institute of Legal Medicine and Forensic Sciences of Portugal, analyzing several variables: age, gender, marital status, employment status, suicidal methodology, toxicological analysis and some conditions/behaviors regarding personal history (alcoholism, suicidal ideation, suicide attempts, physical illness, psychiatric disorder). All the autopsies from the 1 January, 2003 to 31 December, 2009 were analyzed. The suicide profile achieved corresponded to a man (77%), aged between 65 and 74 years old (20.4%), married (54.5%), employed, who committed suicide by hanging, in September, May or February. Clinical records include an organic health problem or psychiatric one, in addition to risk behaviors such as alcoholism, suicidal thoughts or suicide attempts. The number of suicides autopsied at the Centre Branch has increased, resembling the profile to the result of many other authors. However, new medical and social developments place hanging as the favorite suicide method in our study. Many barriers remain to overthrow but several prevention programs begin to be designed and implemented. Future evaluations and interventions at the social and medical level, including the death certification process, will be fundamental to a better realistic understanding of this phenomenon. PMID:24378298

  16. Prevalence of asthma in Portugal - The Portuguese National Asthma Survey

    PubMed Central

    2012-01-01

    Background Asthma is a frequent chronic respiratory disease in both children and adults. However, few data on asthma prevalence are available in Portugal. The Portuguese National Asthma Survey is the first nationwide study that uses standardized methods. We aimed to estimate the prevalence of current asthma in the Portuguese population and to assess the association between ‘Current asthma’ and comorbidities such as upper airways disease. Methods A cross-sectional, population-based, telephone interview survey including all municipalities of Portugal was undertaken. Participants were randomly selected to answer a questionnaire based on the Portuguese version of the GA2LEN survey. ‘Current asthma’ was defined as self-reported lifetime asthma and at least one of 3 symptoms in the last 12 months: wheezing, waking with breathlessness or having an asthma attack. Results Data were obtained for 6 003 respondents, with mean age of 38.9 (95%CI 38.2-39.6) years and 57.3% females. In the Portuguese population, the prevalence of ‘Current asthma’ was 6.8% (95%CI 6.0-7.7) and of ‘Lifetime asthma’ was 10.5% (95%CI 9.5-11.6) Using GA2LEN definition for asthma, our prevalence estimate was 7.8% (95%CI 7.0-8.8). Rhinitis had a strong association with asthma (Adjusted OR 3.87, 95%CI 2.90-5.18) and the association between upper airway diseases and asthma was stronger in patients with both rhinitis and sinusitis (Adjusted OR 13.93, 95%CI 6.60-29.44). Conclusions Current asthma affects 695 000 Portuguese, with a prevalence of 6.8%. People who reported both rhinitis and sinusitis had the highest risk of having asthma. PMID:22931550

  17. Thromboprophylaxis in critically ill children in Spain and Portugal

    PubMed Central

    Nñnez, A. Rodríguez; Fonte, M.; Faustino, E.V.S.

    2015-01-01

    Introduction Although critically ill children may be at risk from developing deep venous thrombosis (DVT), data on its incidence and effectiveness of thromboprophylaxis are lacking. Objective To describe the use of thromboprophylaxis in critically ill children in Spain and Portugal, and to compare the results with international data. Material and methods Secondary analysis of the multinational study PROTRACT, carried out in 59 PICUs from 7 developed countries (4 from Portugal and 6 in Spain). Data were collected from patients less than 18 years old, who did not receive therapeutic thromboprophylaxis. Results A total of 308 patients in Spanish and Portuguese (Iberian) PICUS were compared with 2176 admitted to international PICUs. Risk factors such as femoral vein (P = .01), jugular vein central catheter (P < .001), cancer (P = .03), and sepsis (P < .001), were more frequent in Iberian PICUs. The percentage of patients with pharmacological thromboprophylaxis was similar in both groups (15.3% vs. 12.0%). Low molecular weight heparin was used more frequently in Iberian patients (P < .001). In treated children, prior history of thrombosis (P = .02), femoral vein catheter (P < .001), cancer (P = .02) and cranial trauma or craniectomy (P = .006), were more frequent in Iberian PICUs. Mechanical thromboprophylaxis was used in only 6.8% of candidates in Iberian PICUs, compared with 23.8% in the international PICUs (P < .001). Conclusions Despite the presence of risk factors for DVT in many patients, thromboprophylaxis is rarely prescribed, with low molecular weight heparin being the most used drug. Passive thromboprophylaxis use is anecdotal. There should be a consensus on guidelines of thromboprophylaxis in critically ill children. PMID:24907863

  18. Linguistic isolates in Portugal: insights from the mitochondrial DNA pattern.

    PubMed

    Mairal, Quim; Santos, Cristina; Silva, Marina; Marques, Sofia L; Ramos, Amanda; Aluja, Maria Pilar; Amorim, Antonio; Prata, Maria João; Alvarez, Luis

    2013-12-01

    Miranda do Douro, located in the northeastern region of Portugal, has notable characteristics not only from a geographic or naturalistic point of view, but also from a cultural perspective. A remarkable one is the coexistence of two different languages: Portuguese and Mirandese, the second being an Astur-Leonese dialect. The current persistence of the Astur-Leonese dialect in this population falls on the singularity of the region: relative isolation, implying difficulties to communicate with other Portuguese regions, while the same location facilitated the establishment of social and commercial relationships with adjacent Spanish territories, origin of the Astur-Leonese language. The objective of this study was to characterize the population from Miranda through the analysis of maternal lineages in order to evaluate whether its mitochondrial DNA diversity fitted the patterns previously reported for other populations from the Iberian Peninsula. Viewing that, the entire control region of mitochondrial DNA from 121 individuals was examined. Miranda showed a haplogroup composition usual for a Western European population, in the sense that as high as 63.6% of sequences belonged to macro-haplogroup R0. Lineages ascribed to have an African (L2a and L1b) origin, were detected, but reaching an amount commonly found in Portugal. Miranda also presented a few haplogroups typically found in Jewish populations, while rarely observed in other Iberian populations. The finding can be explained by gene flow with crypto-Jew communities that since long are known to be established in the region where Miranda is located. In Miranda, both genetic and nucleotide diversities presented low values (0.9292 ± 0.0180 and 0.01101 ± 0.00614 respectively) when compared to populations from its micro-geographical framework, which constitute a sign of population isolation that certainly provided conditions for the survival of the Astur-Leonese dialect in the region. PMID:24041913

  19. Spatio-temporal clustering of wildfires in Portugal

    NASA Astrophysics Data System (ADS)

    Costa, R.; Pereira, M. G.; Caramelo, L.; Vega Orozco, C.; Kanevski, M.

    2012-04-01

    Several studies have shown that wildfires in Portugal presenthigh temporal as well as high spatial variability (Pereira et al., 2005, 2011). The identification and characterization of spatio-temporal clusters contributes to a comprehensivecharacterization of the fire regime and to improve the efficiency of fire prevention and combat activities. The main goalsin this studyare: (i) to detect the spatio-temporal clusters of burned area; and, (ii) to characterize these clusters along with the role of human and environmental factors. The data were supplied by the National Forest Authority(AFN, 2011) and comprises: (a)the Portuguese Rural Fire Database, PRFD, (Pereira et al., 2011) for the 1980-2007period; and, (b) the national mapping burned areas between 1990 and 2009. In this work, in order to complement the more common cluster analysis algorithms, an alternative approach based onscan statistics and on the permutation modelwas used. This statistical methodallows the detection of local excess events and to test if such an excess can reasonably have occurred by chance.Results obtained for different simulations performed for different spatial and temporal windows are presented, compared and interpreted.The influence of several fire factors such as (climate, vegetation type, etc.) is also assessed. Pereira, M.G., Trigo, R.M., DaCamara, C.C., Pereira, J.M.C., Leite, S.M., 2005:"Synoptic patterns associated with large summer forest fires in Portugal".Agricultural and Forest Meteorology. 129, 11-25. Pereira, M. G., Malamud, B. D., Trigo, R. M., and Alves, P. I.: The history and characteristics of the 1980-2005 Portuguese rural fire database, Nat. Hazards Earth Syst. Sci., 11, 3343-3358, doi:10.5194/nhess-11-3343-2011, 2011 AFN, 2011: AutoridadeFlorestalNacional (National Forest Authority). Available at http://www.afn.min-agricultura.pt/portal.

  20. The UK Functional Assessment Measure (UK FIM+FAM): Psychometric Evaluation in Patients Undergoing Specialist Rehabilitation following a Stroke from the National UK Clinical Dataset.

    PubMed

    Nayar, Meenakshi; Vanderstay, Roxana; Siegert, Richard J; Turner-Stokes, Lynne

    2016-01-01

    The UK Functional Assessment Measure (UKFIM+FAM) is the principal outcome measure for the UK Rehabilitation Outcomes Collaborative (UKROC) national database for specialist rehabilitation. Previously validated in a mixed neurorehabilitation cohort, this study is the first to explore its psychometric properties in a stroke population, and compare left and right hemispheric strokes (LHS vs RHS). We analysed in-patient episode data from 62 specialist rehabilitation units collated through the UKROC database 2010-2013. Complete data were analysed for 1,539 stroke patients (LHS: 588, RHS: 566 with clear localisation). For factor analysis, admission and discharge data were pooled and randomised into two equivalent samples; the first for exploratory factor analysis (EFA) using principal components analysis, and the second for confirmatory factor analysis (CFA). Responsiveness for each subject (change from admission to discharge) was examined using paired t-tests and differences between LHS and RHS for the entire group were examined using non-paired t-tests. EFA showed a strong general factor accounting for >48% of the total variance. A three-factor solution comprising motor, communication and psychosocial subscales, accounting for >69% total variance, provided acceptable fit statistics on CFA (Root Mean Square Error of Approximation was 0.08 and Comparative Fit Index/ Tucker Lewis Index 0.922/0.907). All three subscales showed significant improvement between admission and discharge (p<0.001) with moderate effect sizes (>0.5). Total scores between LHS and RHS were not significantly different. However, LHS showed significantly higher motor scores (Mean 5.7, 95%CI 2.7, 8.6 p<0.001), while LHS had significantly lower cognitive scores, primarily in the communication domain (-6.8 95%CI -7.7, -5.8 p<0.001). To conclude, the UK FIM+FAM has a three-factor structure in stroke, similar to the general neurorehabilitation population. It is responsive to change during in

  1. The UK Functional Assessment Measure (UK FIM+FAM): Psychometric Evaluation in Patients Undergoing Specialist Rehabilitation following a Stroke from the National UK Clinical Dataset

    PubMed Central

    Nayar, Meenakshi; Vanderstay, Roxana; Siegert, Richard J.; Turner-Stokes, Lynne

    2016-01-01

    The UK Functional Assessment Measure (UKFIM+FAM) is the principal outcome measure for the UK Rehabilitation Outcomes Collaborative (UKROC) national database for specialist rehabilitation. Previously validated in a mixed neurorehabilitation cohort, this study is the first to explore its psychometric properties in a stroke population, and compare left and right hemispheric strokes (LHS vs RHS). We analysed in-patient episode data from 62 specialist rehabilitation units collated through the UKROC database 2010–2013. Complete data were analysed for 1,539 stroke patients (LHS: 588, RHS: 566 with clear localisation). For factor analysis, admission and discharge data were pooled and randomised into two equivalent samples; the first for exploratory factor analysis (EFA) using principal components analysis, and the second for confirmatory factor analysis (CFA). Responsiveness for each subject (change from admission to discharge) was examined using paired t-tests and differences between LHS and RHS for the entire group were examined using non-paired t-tests. EFA showed a strong general factor accounting for >48% of the total variance. A three-factor solution comprising motor, communication and psychosocial subscales, accounting for >69% total variance, provided acceptable fit statistics on CFA (Root Mean Square Error of Approximation was 0.08 and Comparative Fit Index/ Tucker Lewis Index 0.922/0.907). All three subscales showed significant improvement between admission and discharge (p<0.001) with moderate effect sizes (>0.5). Total scores between LHS and RHS were not significantly different. However, LHS showed significantly higher motor scores (Mean 5.7, 95%CI 2.7, 8.6 p<0.001), while LHS had significantly lower cognitive scores, primarily in the communication domain (-6.8 95%CI -7.7, -5.8 p<0.001). To conclude, the UK FIM+FAM has a three-factor structure in stroke, similar to the general neurorehabilitation population. It is responsive to change during in

  2. Hereditary anaemias in Portugal: epidemiology, public health significance, and control.

    PubMed Central

    Martins, M C; Olim, G; Melo, J; Magalhães, H A; Rodrigues, M O

    1993-01-01

    A countrywide prospective study aimed at establishing the prevalence of the haemoglobinopathy genes in the Portuguese population was carried out by screening 15,208 randomly selected blood samples from young males. This male based survey provided the opportunity of assessing simultaneously the prevalence of the red cell enzyme glucose-6-phosphate dehydrogenase (G6PD) deficiency, thus giving a picture of these important hereditary anaemias in Portugal. The results showed a low average frequency of beta thalassaemia (0.45%) and haemoglobin S (0.32%) carriers as well as G6PD deficiency (0.51%). However, these disorders are unevenly distributed throughout the country with a higher prevalence in some areas, mainly in the south. The relationship of this pattern of haemoglobinopathies to the known haplotypes linked to beta thalassaemia and sickle cell disease, relevant historical events, and local selective pressure was investigated. Hb D and Hb J are the commonest other structural variants. The implemented programme for control of these hereditary anaemias is described. PMID:8474108

  3. Anaplasma marginale and Theileria annulata in questing ticks from Portugal.

    PubMed

    Antunes, S; Ferrolho, J; Domingues, N; Santos, A S; Santos-Silva, M M; Domingos, A

    2016-09-01

    Ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. Monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. In this study 428 host-seeking ticks, belonging to nine species of Ixodidae and collected from 17 of the 23 Portuguese mainland subregions, were screened for several tick-borne agents with veterinary relevance: Anaplasma marginale, Anaplasma ovis, Anaplasma centrale, Babesia spp., Coxiella burnetii and Theileria spp. Prevalence was assessed by PCR and amplified amplicons sequenced for validation of results. Twenty ticks, in a total of 428, were found positive: one Ixodes ventalloi for Theileria annulata and four Dermacentor marginatus, one Haemaphysalis punctata, five Ixodes ricinus, five I. ventalloi, and four Rhipicephalus sanguineus sensu lato for A. marginale. According to the reviewed literature, this is the first report of A. marginale and T. annulata detection in I. ventalloi. Furthermore, the amplification of A. marginale DNA in several tick species suggests a broad range for this agent in Portugal that might include other uncommon species as R. sanguineus s.l. This work provides new data towards a better understanding of tick-pathogen associations and also contributes to the surveillance of tick-borne agents in geographic areas with limited information. PMID:27394441

  4. Isolation of Besnoitia besnoiti from infected cattle in Portugal.

    PubMed

    Cortes, H C E; Reis, Y; Waap, H; Vidal, R; Soares, H; Marques, I; Pereira da Fonseca, I; Fazendeiro, I; Ferreira, M L; Caeiro, V; Shkap, V; Hemphill, A; Leitão, A

    2006-11-01

    Besnoitia besnoiti, an obligate intracellular protozoan parasite belonging to the phylum apicomplexa, is the causative agent of bovine besnoitiosis. Besnoitiosis is responsible for significant losses in the cattle industry of Africa and Mediterranean countries due to the high morbidity rate, abortion and infertility in males. The acute stage of disease is associated with the proliferative forms (tachyzoites) and is characterized by fever, whimpery, general weakness and swelling of the superficial lymph nodes. During the following chronic stage, a huge number of cysts are formed mainly in the subcutaneous tissues. This process is non-reversible, and chronic besnoitiosis is characterized by hyper-sclerodermia, hyperkeratosis, alopecia and, in bulls, atrophy, sclerosis and focal necrosis that cause irreversible lesions in the testis. In this paper we report on the identification of large cysts in the skin of a cow and a bull in Portugal, which presented loss of hair and enlargement and pachydermis all over the body. The observation of a two-layered cyst wall within the host cell, the encapsulation of the host cell by a large outer cyst wall, and the subcutaneous localization of the cysts within the host, were characteristic for B. besnoiti. The parasites were isolated from the infected animals and successfully propagated in Vero cells without prior passages in laboratory animals. Morphological characterization of B. besnoiti tachyzoites and the amplification of the 149 bp segment from the internal transcribed spacer 1 (ITS1), aided with specific primers, confirmed the identification of B. besnoiti. PMID:16822614

  5. Burkholderia pseudomallei: First case of melioidosis in Portugal.

    PubMed

    Pelerito, Ana; Nunes, Alexandra; Coelho, Susana; Piedade, Cátia; Paixão, Paulo; Cordeiro, Rita; Sampaio, Daniel; Vieira, Luís; Gomes, João Paulo; Núncio, Sofia

    2016-01-01

    Burkholderia pseudomallei is a Gram-negative bacillus and the causative agent of melioidosis, a serious infection associated with high mortality rate in humans. It can be naturally found as an environmental saprophyte in soil or stagnant water, and rice paddies that predominate in regions of endemicity such as Northeast Thailand. B. pseudomallei is a Biosafety Level 3 organism due to risks of aerosolization and severe disease and is now included in formal emergency preparedness plans and guidelines issued by various authorities in the United States and Europe. Here, we report the first case of imported melioidosis in Portugal. B. pseudomallei was isolated from the patient's blood as well as from a left gluteal abscess pus. The isolate strain showed the unusual resistance profile to first-line eradication therapy trimethroprim/sulfamethoxazole. Whole genome sequencing revealed its similarity with isolates from Southeast Asia, suggesting the Thai origin of this Portuguese isolate, which is in agreement with a recent patient's travel to Thailand. PMID:26962474

  6. Evaluation of compliance with national legislation on emissions in Portugal.

    PubMed

    Gomes, João F P

    2005-04-01

    More than 13 years after publication of the first air quality laws in Portugal and more than 10 years after the publication of the respective emission limits, it seems appropriate to analyze the degree of compliance by the Portuguese manufacturing industry. Using the data from emission measurements made regularly by the Instituto de Soldadura e Qualidade, the only officially accredited laboratory according to standard ISO 17025, I analyzed a set of approximately 400 sources in terms of compliance with the emission limits regarding total suspended particulates, sulfur dioxide, nitrogen oxides, and volatile organic compounds. I evaluated compliance through a nondimensional parameter and plotted it versus the emission flow rate to derive conclusions: the results indicate that emission limits are generally met regarding sulfur dioxide and nitrogen oxides but not for the other pollutants considered in this study. However, noncompliance occurs mainly for very low emission flow rates, which suggests some alterations in the emission limits, which are being revised at the moment. These alterations will include the exemption of measurements in minor sources. PMID:15887892

  7. The Great Recession in Portugal: impact on hospital care use.

    PubMed

    Perelman, Julian; Felix, Sónia; Santana, Rui

    2015-03-01

    The Great Recession started in Portugal in 2009, coupled with severe austerity. This study examines its impact on hospital care utilization, interpreted as caused by demand-side effects (related to variations in population income and health) and supply-side effects (related to hospitals' tighter budgets and reduced capacity). The database included all in-patient stays at all Portuguese NHS hospitals over the 2001-2012 period (n=17.7 millions). We analyzed changes in discharge rates, casemix index, and length of stay (LOS), using a before-after methodology. We additionally measured the association of health care indicators to unemployment. A 3.2% higher rate of discharges was observed after 2009. Urgent stays increased by 2.5%, while elective in-patient stays decreased by 1.4% after 2011. The LOS was 2.8% shorter after the crisis onset, essentially driven by the 4.5% decrease among non-elective stays. A one percentage point increase in unemployment rate was associated to a 0.4% increase in total volume, a 2.3% decrease in day cases, and a 0.1% decrease in LOS. The increase in total and urgent cases may reflect delayed out-patient care and health deterioration; the reduced volume of elective stays possibly signal a reduced capacity; finally, the shorter stays may indicate either efficiency-enhancing measures or reduced quality. PMID:25583679

  8. [A sociography of elderly victims of family violence in Portugal].

    PubMed

    Gil, Ana Paula; Santos, Ana João; Kislaya, Irina; Santos, César; Mascoli, Luísa; Ferreira, Alexandra Inácio; Vieira, Duarte Nuno

    2015-06-01

    This article presents data on violence against persons aged 60 years or older and living in Portugal for at least 12 months. The cross-sectional descriptive study collected data with a face-to-face questionnaire applied to victims that had sought assistance at three government institutions and one non-governmental support service for victims. The non-probabilistic sample included 510 victims of violence in family settings. Physical and psychological violence were the most common (87.8% and 69.6%, respectively). Financial violence was reported by 47.5% of the victims, followed by sexual assault (7.5%) and neglect (6.5%). The majority of victims (74.1%) reported more than one type of violence. Victims were mostly women, and mean age was 70.7 years. Most of the perpetrators belonged to the nuclear family, namely spouses or partners, sons/sons-in-law, and daughters/daughters-in-law. However, differences were observed according to victim's gender. The results indicate that violence is not a uniform phenomenon, presenting different configurations. PMID:26200371

  9. A strategic approach for Water Safety Plans implementation in Portugal.

    PubMed

    Vieira, Jose M P

    2011-03-01

    Effective risk assessment and risk management approaches in public drinking water systems can benefit from a systematic process for hazards identification and effective management control based on the Water Safety Plan (WSP) concept. Good results from WSP development and implementation in a small number of Portuguese water utilities have shown that a more ambitious nationwide strategic approach to disseminate this methodology is needed. However, the establishment of strategic frameworks for systematic and organic scaling-up of WSP implementation at a national level requires major constraints to be overcome: lack of legislation and policies and the need for appropriate monitoring tools. This study presents a framework to inform future policy making by understanding the key constraints and needs related to institutional, organizational and research issues for WSP development and implementation in Portugal. This methodological contribution for WSP implementation can be replicated at a global scale. National health authorities and the Regulator may promote changes in legislation and policies. Independent global monitoring and benchmarking are adequate tools for measuring the progress over time and for comparing the performance of water utilities. Water utilities self-assessment must include performance improvement, operational monitoring and verification. Research and education and resources dissemination ensure knowledge acquisition and transfer. PMID:21301119

  10. Salt Fluxes in a Complex River Mouth System of Portugal

    PubMed Central

    Vaz, Nuno; Lencart e Silva, João D.; Dias, João Miguel

    2012-01-01

    Measurements of velocity and salinity near the mouth and head of the Espinheiro channel (Ria de Aveiro lagoon, Portugal) are used to study the local variation of physical water properties and to assess the balance, under steady conditions, between the seaward salt transport induced by river discharge and the landward dispersion induced by various mixing mechanisms. This assessment is made using data sampled during complete tidal cycles. Under the assumption that the estuarine tidal channel is laterally homogeneous and during moderate tidal periods (except for one survey), currents and salinity data were decomposed into various spatial and temporal means and their deviations. Near the channel's mouth, the main contributions to the salt transport are the terms due to freshwater discharge and the tidal correlation. Near the channel's head, this last term is less important than the density driven circulation, which is enhanced by the increase in freshwater discharge. The remaining terms, which are dependent on the deviations from the mean depth have a smaller role in the results of salt transport. The computed salt transport per unit width of a section perpendicular to the mean flow is in close agreement to the sum of the advective and dispersive terms (within or very close to 12%). An imbalance of the salt budget across the sections is observed for all the surveys. Considerations are made on how this approach can inform the management of hazardous contamination and how to use these results to best time the release of environmental flows during dry months. PMID:23071793

  11. Distribution of materials in construction and demolition waste in Portugal.

    PubMed

    Coelho, André; de Brito, Jorge

    2011-08-01

    It may not be enough simply to know the global volume of construction and demolition waste (CDW) generated in a certain region or country if one wants to estimate, for instance, the revenue accruing from separating several types of materials from the input entering a given CDW recycling plant. A more detailed determination of the distribution of the materials within the generated CDW is needed and the present paper addresses this issue, distinguishing different buildings and types of operation (new construction, retrofitting and demolition). This has been achieved by measuring the materials from buildings of different ages within the Portuguese building stock, and by using direct data from demolition/retrofitting sites and new construction average values reported in the literature. An attempt to establish a benchmark with other countries is also presented. This knowledge may also benefit industry management, especially that related to CDW recycling, helping to optimize procedures, equipment size and operation and even industrial plant spatial distribution. In an extremely competitive market, where as in Portugal low-tech and high environmental impact procedures remain the norm in the construction industry (in particular, the construction waste industry), the introduction of a successful recycling industry is only possible with highly optimized processes and based on a knowledge-based approach to problems. PMID:20498131

  12. Female independence in Portugal: effect on suicide rates.

    PubMed

    de Castro, E F; Pimenta, F; Martins, I

    1988-08-01

    The greater incidence of suicide among males could be ascribed to the distinct roles still attributed to each sex. Progress towards female independence could reduce this different incidence. We analysed the following in Portugal: male and female suicide rates; profiles; and male/female suicide ratio before (1955-1969) and after the development of a movement for women's independence (1970-85). Concomitant with progress towards female independence there is a significant rise in female suicide and a decrease in male/female suicide ratio. The highest rates are among professional/technical women living in urban areas. In professional groups there is significant correlation between deaths caused by suicide and by liver cirrhosis. It is concluded that alcoholism often leads to suicide; in women, taboos about alcoholism and suicide explain a higher incidence of suicide among culturally freer professional groups; female independence will catalyse a rise in alcoholism, which together with other factors resulting from that independence will lead to a predictable increase of suicide among Portuguese women and a reduced difference in rates of suicide between the sexes. PMID:3265573

  13. Granite geomorphology and its geological controls, Serra da Estrela, Portugal

    NASA Astrophysics Data System (ADS)

    Migoń, Piotr; Vieira, Gonçalo

    2014-12-01

    Serra da Estrela is an elevated granite massif in central Portugal, characterized by extensive plateau surfaces incised by deep valleys affected by Quaternary glaciation, bounded by steep fault-generated escarpments. The presence of seven major textural variants of granite provides an opportunity to study the relationships between lithology and relief, whereas DEM analysis helped to show the relationships between lithology and topography objectively. The higher ground is associated with fine- to medium-grained granites and is typified by planar surfaces of low gradient, with occasional angular tors and rock pedestals. Block fields built by angular material are common in the parts that were not previously glaciated. Less elevated parts of the plateau are supported by medium- to coarse-grained granites and show more varied topography, with an abundance of tors, boulder piles, and depressions. Lithological boundaries locally coincide with slope breaks but this is not the rule. In the northern part of the massif a deep topographic basin has evolved in biotite granite, whereas deeply incised valleys follow major fault lines. Geological controls show a hierarchy, in that gross relief reflects the pattern of tectonic uplift and subsidence, whereas lithology and then fracture patterns become more and more important if one focuses on smaller and smaller landforms.

  14. Exploratory study of air quality in elementary schools, Coimbra, Portugal

    PubMed Central

    Ferreira, Ana Maria Conceição; Cardoso, Salvador Massano

    2013-01-01

    OBJECTIVE To analyze the air quality in elementary schools and their structural and functional conditions. METHODS Air quality in 51 elementary schools (81 classrooms) in the city of Coimbra, Portugal, both inside and outside of the rooms was evaluated during the four seasons, from 2010 to 2011. Temperature (T°), relative humidity (Hr), concentrations of carbon monoxide (CO), carbon dioxide (CO2), ozone (O3), nitrogen dioxide (NO2), sulfur dioxide (SO2), compounds were evaluated, as were volatile organics (VOC), formaldehyde and particulate matter (PM10), from November 2010 to February 2011 (autumn/winter) and March 2011 to June 2011 (spring/summer). A grid characterizing the structural and functional conditions of the schools was created. The statistical Student t test for paired samples and the Wilcoxon t test were applied. RESULTS In 47 schools, the average CO2concentrations were above the maximum reference concentration (984 ppm) mentioned in Portuguese legislation. The maximum concentration values found inside the rooms were critical, especially in the fall/winter (5,320 ppm). In some schools the average concentrations of VOC and PM10within the maximum concentration exceeded the reference legislated. The values (risk) of CO, formaldehyde, NO2, SO2and O3detected were not relevant. CONCLUSIONS There was a higher concentration of pollutants inside the rooms compared with outside. Inadequate ventilation is associated with high CO2concentration in the classroom. PMID:24626544

  15. Litter in submarine canyons off the west coast of Portugal

    NASA Astrophysics Data System (ADS)

    Mordecai, Gideon; Tyler, Paul A.; Masson, Douglas G.; Huvenne, Veerle A. I.

    2011-12-01

    Marine litter is of global concern and is present in all the world's oceans, including deep benthic habitats where the extent of the problem is still largely unknown. Litter abundance and composition were investigated using video footage and still images from 16 Remotely Operated Vehicle (ROV) dives in Lisbon, Setúbal, Cascais and Nazaré Canyons located west of Portugal. Litter was most abundant at sites closest to the coastline and population centres, suggesting the majority of the litter was land sourced. Plastic was the dominant type of debris, followed by fishing gear. Standardised mean abundance was 1100 litter items km -2, but was as high as 6600 litter items km -2 in canyons close to Lisbon. Although all anthropogenic material may be harmful to biota, debris was also used as a habitat by some macro-invertebrates. Litter composition and abundance observed in the canyons of the Portuguese margin were comparable to those seen in other deep sea areas around the world. Accumulation of litter in the deep sea is a consequence of human activities both on land and at sea. This needs to be taken into account in future policy decisions regarding marine pollution.

  16. Burkholderia pseudomallei: First case of melioidosis in Portugal

    PubMed Central

    Pelerito, Ana; Nunes, Alexandra; Coelho, Susana; Piedade, Cátia; Paixão, Paulo; Cordeiro, Rita; Sampaio, Daniel; Vieira, Luís; Gomes, João Paulo; Núncio, Sofia

    2016-01-01

    Burkholderia pseudomallei is a Gram-negative bacillus and the causative agent of melioidosis, a serious infection associated with high mortality rate in humans. It can be naturally found as an environmental saprophyte in soil or stagnant water, and rice paddies that predominate in regions of endemicity such as Northeast Thailand. B. pseudomallei is a Biosafety Level 3 organism due to risks of aerosolization and severe disease and is now included in formal emergency preparedness plans and guidelines issued by various authorities in the United States and Europe. Here, we report the first case of imported melioidosis in Portugal. B. pseudomallei was isolated from the patient's blood as well as from a left gluteal abscess pus. The isolate strain showed the unusual resistance profile to first-line eradication therapy trimethroprim/sulfamethoxazole. Whole genome sequencing revealed its similarity with isolates from Southeast Asia, suggesting the Thai origin of this Portuguese isolate, which is in agreement with a recent patient's travel to Thailand. PMID:26962474

  17. Practice of biopsychosocial medicine in Portugal: perspectives of professionals involved.

    PubMed

    Pereira, M Graça; Fachada, Alfonso Alonso; Smith, Thomas Edward

    2009-05-01

    Although, recently, the biopsychosocial approach has been emphasized in the practice of family medicine, how psychologists and physicians interact in collaborative family health care practice is still emerging in Portugal. This article describes a qualitative study that focused on the understanding of psychologists and family physicians' perceptions of their role and the collaborative approach in health care. A questionnaire gathered information regarding collaboration, referral, training and the practice of biopsychosocial medicine. A content analysis on respondents' discourse was performed. Results show that both physicians and psychologists agree on the importance of the biopsychosocial model and interdisciplinary collaboration. However, they also mentioned several difficulties that have to do with the lack of psychologists working full time in health care centers, lack of communication and different expectancies regarding each other roles in health care delivery. Both physicians and psychologists acknowledge the lack of academic training and consider the need for multidisciplinary teams in their training and practice to improve collaboration and integrative care. Implications for future research and for the practice of biopsychosocial medicine are addressed. PMID:19476234

  18. New insights into the reconstructed temperature in Portugal over the last 400 years

    NASA Astrophysics Data System (ADS)

    Santos, J. A.; Carneiro, M. F.; Correia, A.; Alcoforado, M. J.; Zorita, E.; Gómez-Navarro, J. J.

    2015-06-01

    The consistency of an existing reconstructed annual (December-November) temperature series for the Lisbon region (Portugal) from 1600 onwards, based on a European-wide reconstruction, with (1) five local borehole temperature-depth profiles; (2) synthetic temperature-depth profiles, generated from both reconstructed temperatures and two regional paleoclimate simulations in Portugal; (3) instrumental data sources over the twentieth century; and (4) temperature indices from documentary sources during the late Maunder Minimum (1675-1715) is assessed. The low-frequency variability in the reconstructed temperature in Portugal is not entirely consistent with local borehole temperature-depth profiles and with the simulated response of temperature in two regional paleoclimate simulations driven by reconstructions of various climate forcings. Therefore, the existing reconstructed series is calibrated by adjusting its low-frequency variability to the simulations (first-stage adjustment). The annual reconstructed series is then calibrated in its location and scale parameters, using the instrumental series and a linear regression between them (second-stage adjustment). This calibrated series shows clear footprints of the Maunder and Dalton minima, commonly related to changes in solar activity and explosive volcanic eruptions, and a strong recent-past warming, commonly related to human-driven forcing. Lastly, it is also in overall agreement with annual temperature indices over the late Maunder Minimum in Portugal. The series resulting from this post-reconstruction adjustment can be of foremost relevance to improve the current understanding of the driving mechanisms of climate variability in Portugal.

  19. The Eclipse, the Astronomer and His Audience: Frederico Oom and the Total Solar Eclipse of 28 May 1900 in Portugal

    ERIC Educational Resources Information Center

    Carolino, Luis Miguel; Simoes, Ana

    2012-01-01

    This study offers a detailed analysis of an episode of the popularization of astronomy which took place in Portugal, a peripheral country of Europe, and occurring in the early twentieth century. The episode was driven by the 28 May 1900 total solar eclipse which was seen on the Iberian Peninsula (Portugal and Spain). Instead of focusing on one of…

  20. Genetic characterization of Toxoplasma gondii isolates from Portugal, Austria, and Israel reveals higher genetic variability within the type II lineage

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study compared genetic diversity of Toxoplasma gondii isolates from Portugal, Austria and Israel. For this, we genotyped 90 T. gondii isolates (16 from Portugal, 67 from Austria and 7 from Israel) using 10 nested PCR-restriction length polymorphism (RFLP) genetic markers and 15 microsatellite (...

  1. The impact of winter cold weather on acute myocardial infarctions in Portugal.

    PubMed

    Vasconcelos, João; Freire, Elisabete; Almendra, Ricardo; Silva, Giovani L; Santana, Paula

    2013-12-01

    Mortality due to cardiovascular diseases shows a seasonal trend that can be associated with cold weather. Portugal is the European country with the highest excess winter mortality, but nevertheless, the relationship between cold weather and health is yet to be assessed. The main aim of this study is to identify the contribution of cold weather to cardiovascular diseases within Portugal. Poisson regression analysis based on generalized additive models was applied to estimate the influence of a human-biometeorological index (PET) on daily hospitalizations for myocardial infarction. The main results revealed a negative effect of cold weather on acute myocardial infarctions in Portugal. For every degree fall in PET during winter, there was an increase of up to 2.2% (95% CI = 0.9%; 3.3%) in daily hospital admissions. This paper shows the need for public policies that will help minimize or, indeed, prevent exposure to cold. PMID:23410618

  2. Surveillance of norovirus in Portugal and the emergence of the Sydney variant, 2011-2013.

    PubMed

    Costa, I; Mesquita, J R; Veiga, E; Oleastro, M; Nascimento, M J S

    2015-09-01

    This report presents the results of the national surveillance system of diarrhea etiology of the National Institute of Health of Portugal concerning norovirus (NoV) during a two-year period, May 2011-2013. Of the total 580 stool samples collected from patients hospitalized for acute diarrhea in 13 Hospitals of Portugal, 67 (11.6%) tested positive for NoV. From May 2011 to March 2012 the GII.4 variant New Orleans 2009 was the most predominant strain having been replaced by the new GII.4 variant Sydney 2012 since then till the end of the survey. To our knowledge this is the first study showing the circulation of GII.4 as the norovirus strain most commonly associated to gastroenteritis and the first to report the replacement of GII.4 New Orleans by GII.4 Sydney 2012 variant in Portugal. PMID:26305815

  3. Mathematics diagnostic testing in engineering: an international comparison between Ireland and Portugal

    NASA Astrophysics Data System (ADS)

    Carr, M.; Fidalgo, C.; Bigotte de Almeida, M. E.; Branco, J. R.; Santos, V.; Murphy, E.; Fhloinn, E. Ní

    2015-09-01

    Concern has been expressed throughout Europe about the significant deficiencies in the basic mathematical skills of many engineering undergraduates. Mathematics diagnostic tests in the UK, Ireland and Portugal have shown these shortcomings, which provide a challenge to those striving to introduce more innovative educational practices into engineering education, such as projects or real-world problems. Every year, in the Dublin Institute of Technology (Ireland) and the Instituto Superior de Engenharia de Coimbra (Portugal), a diagnostic test is given to incoming first-year students. A comparison showed some potentially interesting differences between these students. In September 2013, a project was undertaken to compare mathematical competencies of incoming engineering students in both countries. A modified diagnostic test was devised and the results were then compared to ascertain if there are common areas of difficulty between students in Ireland and Portugal, or evidence of one group significantly outperforming the other in a particular area.

  4. Optical fiber sensors: a route from University of Kent to Portugal

    NASA Astrophysics Data System (ADS)

    Santos, José L.; Lobo Ribeiro, António B.

    2011-06-01

    In this work the authors first summarily describe the main topics that were the subject of their post-graduate activity in fiber sensing at the Applied Optics Group of University of Kent in the late 1980s and early 1990s. After their return to Porto, Portugal, the know-how acquired during their stay at Kent and the collaboration paths that followed between the University of Porto and University of Kent were instrumental in the start-up and progress of optical fiber sensing activity in Portugal. The main topics addressed in this field, the description of some of the relevant developments achieved in recent years, the present situation and the guidelines for the future research and development activity in Portugal in fiber sensing will be the core of this work.

  5. Human immunodeficiency virus type 1 recombinant B/G subtypes circulating in Coimbra, Portugal.

    PubMed

    Duque, V; Holguín, A; Silvestre, M; González-Lahoz, J; Soriano, V

    2003-05-01

    An increasing prevalence of HIV-1 non-B variants is being noticed in several European regions, particularly in countries such as Portugal, which have closer contacts with African endemic areas, where multiple HIV subtypes cocirculate. HIV-1 subtyping by phylogenetic analyses of reverse transcriptase, protease and env (C2-V3) genomic regions was carried out in plasma collected from 18 HIV-1-infected subjects living in Coimbra, Portugal, and suspected to be infected with non-B variants. Three (16.7%) subjects carried recombinant B/G viruses (BV3/BRT/Gpro; GV3/URT/Bpro; AV3/GRT/Bpro), whereas all the remaining individuals were infected with HIV-1 subtype B. This is the first report of recombinant B/G subtypes in Portugal. PMID:12848757

  6. Eucisia schist a natural stone from Northern Portugal

    NASA Astrophysics Data System (ADS)

    Aires, Silvia; Carvalho, Cristina; Noronha, Fernando

    2013-04-01

    : CS 53 Mpa, FS 16.3 MPa, AD 25220 kg/m3 , OP 9.3%, WA 1,2 % AR 26.0mm and RTS resistant. For settling the recommended applications, European standards for natural stone products were considered. Considering the technical specifications that exist in some European countries, "Eucisia schist" can be applied on rustic masonary units, on colums, on paving (low traffic, mainly indoors) and on cladding (mainly indoors). This work was performed under the project PTDC/CTE-GIN/70704/2006, "SCHISTRESOURCE funded by FCT (Portuguese Science and Technology Foundation). SFRH/BD/86641/2012 a FCT grant financially supports Silvia Aires. [1] A.Ribeiro, E. Pereira and R. Dias, in Pre-Mesozoic Geology of Iberia edited by R.D. Dallmeyer and E. Martinez-Garcia, Berlin Heidelberg: Springer-Verlag. (1990). [2] E. Pereira, in: E. Pereira, coord. Carta Geológica de Portugal. Notícia Explicativa da Folha 2. (2006) [3] E. Pereira, A. Ribeiro, in: E. Pereira, coord. Carta Geológica de Portugal. Notícia Explicativa da Folha 2. (2006).

  7. Surveillance of imported hospital requiring malaria in Portugal: can it be improved?

    PubMed

    Fonseca, Ana Glória; Simões Dias, Sara; Baptista, João Luís; Torgal, Jorge

    2016-06-01

    Although eradicated in Portugal, malaria keeps taking its toll on travellers and migrants from endemic countries. Completeness of hospital requiring malaria notification in Portugal 2000-11 was estimated, using two-source capture-recapture method. Data sources were: national surveillance database of notifiable diseases and the national database of the Diagnosis-Related Groups resulting from National Health Service (NHS) hospital episodes. The completeness of notification was 21,2% for all malaria cases and 26,5% for malaria deaths, indicating significant underreporting and urging for complementary data source in surveillance, for disease burden estimates and retrospective monitoring, namely hospital episodes statistics. PMID:27069002

  8. Feasibility Studies and Design of High-Speed Railway (TGV) Projects in Portugal

    NASA Astrophysics Data System (ADS)

    Oliveira, Ricardo; Abreu, Rui

    Portugal is starting a large program of high-speed train routes connecting the main cities in the country and these cities to central Europe throughout Spain. The country has a very wide range of lithological types and the main routes cross some important hilly zones. These facts, together with the severe geometric restrictions associated with the High-Speed Trains, attach an important role to Engineering Geology both at the Feasibility stage and at the Design stage of the projects. The paper illustrates this important role with examples of studies currently under way in Portugal.

  9. Risk assessment in submarine outfall projects: the case of Portugal.

    PubMed

    Mendonça, Ana; Losada, Miguel Ángel; Reis, Maria Teresa; Neves, Maria Graça

    2013-02-15

    Submarine outfalls need to be evaluated as part of an integrated environmental protection system for coastal areas. Although outfalls are tight with the diversity of economic activities along a densely populated coastline being effluent treatment and effluent reuse a sign of economic prosperity, precautions must be taken in the construction of these structures. They must be designed so as to have the least possible impact on the environment and at the same time be economically viable. This paper outlines the initial phases of a risk assessment procedure for submarine outfall projects. This approach includes a cost-benefit analysis in which risks are systematically minimized or eliminated. The methods used in this study also allow for randomness and uncertainty. The input for the analysis is a wide range of information and data concerning the failure probability of outfalls and the consequences of an operational stoppage or failure. As part of this risk assessment, target design levels of reliability, functionality, and operationality were defined for the outfalls. These levels were based on an inventory of risks associated with such construction projects, and thus afforded the possibility of identifying possible failure modes. This assessment procedure was then applied to four case studies in Portugal. The results obtained were the values concerning the useful life of the outfalls at the four sites and their joint probability of failure against the principal failure modes assigned to ultimate and serviceability limit states. Also defined were the minimum operationality of these outfalls, the average number of admissible technical breakdowns, and the maximum allowed duration of a stoppage mode. It was found that these values were in consonance with the nature of the effluent (tourist-related, industrial, or mixed) as well as its importance for the local economy. Even more important, this risk assessment procedure was able to measure the impact of the outfalls on

  10. Late Pleistocene raised beaches of coastal Estremadura, central Portugal

    NASA Astrophysics Data System (ADS)

    Benedetti, Michael M.; Haws, Jonathan A.; Funk, Caroline L.; Daniels, J. Michael; Hesp, Patrick A.; Bicho, Nuno F.; Minckley, Thomas A.; Ellwood, Brooks B.; Forman, Steven L.

    2009-12-01

    We present new stratigraphic, sedimentological, and chronological data for a suite of tectonically raised beaches dating to Marine Isotope Stages 5, 4, and 3 along the Estremadura coast of west-central Portugal. The beach deposits are found in association with ancient tidal channels and coastal dunes, pollen bearing mud and peat, and Middle Paleolithic archaeological sites that confirm occupation of the coastal zone by Neanderthal populations. The significance of these deposits is discussed in terms of the archaeological record, the tectonic and geomorphic evolution of the coast, and correlation with reconstructions of global climate and eustatic sea-level change. Direct correlation between the Estremadura beach sections is complicated by the tectonic complexity of the area and the age of the beach deposits (which are near or beyond the limit of radiocarbon dating). Evidence from multiple sites dated by AMS radiocarbon and optical luminescence methods suggests broad synchroneity in relative sea-level changes along this coast during Marine Isotope Stage 3. Two beach complexes with luminescence and radiocarbon age control date to about 35 ka and 42 ka, recording a rise in relative sea level around the time of Heinrich Event 4 at 39 ka. Depending on assumptions about eustatic sea level at the time they were deposited, we estimate that these beaches have been uplifted at rates of 0.4-4.3 mm yr -1 by the combined effects of tectonic, halokinetic, and isostatic processes. Uplift rates of 1-2 mm yr -1 are likely if the beaches represent sea level stands at roughly 40 m below modern, as suggested by recent eustatic sea level reconstructions. Evidence from coastal bluffs and the interior of the study area indicates extensive colluvial, fluvial, and aeolian sedimentation beginning around 31 ka and continuing into the Holocene. These geomorphic adjustments are related to concomitant changes in climate and sea level, providing context that improves our understanding of Late

  11. Modeling fecal contamination in the Aljezur coastal stream (Portugal)

    NASA Astrophysics Data System (ADS)

    Rodrigues, Marta; Oliveira, Anabela; Guerreiro, Martha; Fortunato, André Bustorff; Menaia, José; David, Luís Mesquita; Cravo, Alexandra

    2011-06-01

    This study aims at understanding the fecal contamination behavior in a small coastal stream (Aljezur, Portugal), which has significant economic and ecological values. Like in most small coastal systems, circulation and water renewal in the Aljezur stream exhibit a strong variability due to their dependence on tides, waves, intermittent river flows, and a highly variable morphology. Hence, the problem was approached through a combination of field surveys and the development and application of a hard-coupled three-dimensional hydrodynamic and fecal contamination model. Salinity and temperature results have shown that mixing and transport in the stream are very sensitive to the river flow and wind forcing. The model is able to represent the main patterns and trends observed in Escherichia coli and fecal enterococcus concentrations along the stream, for different environmental and contamination conditions, suggesting die-off rates on the order of 0.50-0.55 day-1. Die-off rate and the representation of the sediment-associated processes were identified as the major remaining sources of uncertainty in the model. Results show that, owing to the processes that occur along the stream, fecal bacteria reach the beaches water in numbers that comply with the European Bathing Waters Directive, even during the summer periods when the upstream concentrations are larger. In particular, results suggest a direct relation between the tidal propagation upstream and the reduction of the fecal bacteria concentrations along the stream that can be relevant for the development of a strategy for the management of the system's water safety.

  12. Rehabilitation of adobe buildings. Understanding different materials from Portugal

    NASA Astrophysics Data System (ADS)

    Costa, Cristiana; Rocha, Fernando; Velosa, Ana

    2016-04-01

    Earth construction is the oldest building material known, with documented cases of the use of earth bricks since Mesopotamia around 10 000 BC (Heathcote, 1995). The earth construction exists throughout the majority of the world in different cultures, and for some countries, nowadays it continues to be the main process of construction (Vega et al, 2011). Around 30% of the world's population lives in buildings made of earth materials. Earthen construction is an environmentally friendly technique with a social and cultural contribution; this advantage is increased when this type of construction is applied in developing countries where the material costs counterbalance with labour costs, and where other materials and techniques cannot be available (Ciancio et al, 2013). Studies of materials characterization are required in order to understand the composition and specific properties of the earth buildings, their heterogeneity and their degradation mechanisms. Some adobes from different buildings, ages and regions of Portugal were collected in order to characterize them (mineralogically, chemically and physically). It was possible to understand the composition of these materials and their differences. Main minerals are quartz, feldspars, calcite and phyllosilicates (mica and kaolinite). The mechanical behaviour of these materials isn't the best, but it is possible to improve it with some simple and cheap natural additives (kaolinitic soils). The characterization of these materials allows us to understand the differences between the materials from the different regions (controlled by locally available raw materials). Understanding these materials, and their properties, it is possible to formulate new ones for repair, conservation and rehabilitation works. The adobe bricks are an alternative of kiln baked bricks which has several advantages and one of the most important is that these materials are recyclable. Adobes are an excellent option for building rehabilitation, if

  13. Occurrence of springs in massifs of crystalline rocks, northern Portugal

    NASA Astrophysics Data System (ADS)

    Pacheco, Fernando António Leal; Alencoão, Ana Maria Pires

    2002-02-01

    An inventory of artesian springs emerging from fractures (fracture springs) was conducted in the Pinhão River Basin and Morais Massif, northern Portugal, comprising an area of approximately 650 km2. Over 1,500 springs were identified and associated with geological domains and fracture sets. Using cross-tabulation analysis, spring distributions by fracture sets were compared among geological environments, and the deviations related to differences in rock structure and, presumably, to differences in deformational histories. The relation between spring frequencies and rock structures was further investigated by spectral determination, the model introduced in this study. Input data are the spring frequencies and fracture lengths in each geological domain, in addition to the angles between fracture strikes and present-day stress-field orientation (θ). The model's output includes the so-called intrinsic densities, a parameter indexing spring occurrence to factors such as fracture type and associated deformational regime and age. The highest densities (12.2 springs/km of lineament) were associated with young shear fractures produced by brittle deformation, and the lowest (0.1) with old tensional and ductile fractures. Spectral determination also relates each orientation class to a dominant structural parameter: where spring occurrence is controlled by θ, the class is parallel to the present-day stress-field orientation; where the control is attributed to the length of fractures, the spring occurrence follows the strike of large-scale normal faults crossing the region. Résumé. Un inventaire des sources artésiennes émergeant de fractures (sources de fractures) a été réalisé dans le bassin de la rivière Pinhão et dans le massif de Morais, dans le nord du Portugal, dans une région couvrant environ 650 km2. Plus de 1,500 sources ont été identifiées et associées à des domaines géologiques et à des ensembles de fractures. Grâce à une analyse de tableaux

  14. Reinterpreting Lifelong Learning: Meanings of Adult Education Policy in Portugal, 1999-2010

    ERIC Educational Resources Information Center

    Guimaraes, Paula

    2013-01-01

    This article analyses Portugal's adult education policy between 1999 and 2010. Our empirical material consists of Portuguese as well as supranational policy documents. We use a theoretical framework which distinguishes three models of public policy, with different views on the roles of public policy and of education: (1) participative…

  15. Teacher Professionalisation and Professionalism in Portugal and Brazil: What Do the Policy Documents Tell?

    ERIC Educational Resources Information Center

    Flores, Maria Assuncao; Shiroma, Enida

    2003-01-01

    Examines teacher education policy in Portugal and Brazil over the past decade, discussing implications for teacher professionalization and professionalism; identifying the emergence of competing discourses arising from policy texts and other formal documents; and asserting that the intertwined concepts of professionalization and professionalism,…

  16. The Democratisation of Access and Success in Higher Education: The Case of Portugal and Brazil

    ERIC Educational Resources Information Center

    Dias, Diana; Marinho-Araujo, Claisy; Almeida, Leandro; Amaral, Alberto

    2011-01-01

    Given that higher education systems everywhere have opened to the masses, this paper analyses to what extent this phenomenon has really been accompanied by an effective democratisation of access and success in Portugal and Brazil. It looks at the expansion of higher education and discusses how the political system and higher education institutions…

  17. Educational Journals and Worldwide Circulation of Pedagogical Knowledge: Brazil and Portugal (1921?1935)

    ERIC Educational Resources Information Center

    Francisco, Jaime; Cordeiro, Parreira; Carvalho, Luis Miguel

    2005-01-01

    This article focuses on the circulation of educational models between Portugal and Brazil (from 1920 to 1935), within a broader context of an intensive international transfer of "ways of thinking" about education. It describes and analyses the results from research on two educational journals, one Portuguese (Revista Escolar) the other from the…

  18. Continuing Vocational Training in Local Government in Portugal, 2000-05--What Has Changed?

    ERIC Educational Resources Information Center

    Cabrito, Belmiro Gil; Simao, Ana Margarida Veiga; Alves, Mariana Gaio; Almeida, Antonio

    2009-01-01

    Local government in Portugal had a good opportunity to modernise through the Programa de Formacao para as Autarquias Locais (Foral) [Training programme for local authorities], implemented between 2000 and 2005. Substantial financial resources were made available through the programme to retrain local government human resources in order to improve…

  19. Translating University-Industry Relations: The Development of a Knowledge Link in Portugal

    ERIC Educational Resources Information Center

    Graca, Manuel

    2005-01-01

    INESC was created in 1980 in Portugal to link universities with industry. Starting within a university as the initiative of three young academics, the organization soon became a pivotal player in the modernization of the country through the development of applied research in science and technology to enhance the competitiveness of Portuguese…

  20. School Effectiveness Research Findings in the Portuguese Speaking Countries: Brazil and Portugal

    ERIC Educational Resources Information Center

    Ferrão, Maria Eugénia

    2014-01-01

    This paper provides findings of research on school effectiveness and discusses implications for evaluation in Brazil and Portugal. Most findings reported over the last decade have been published in Brazilian or Portuguese refereed journals. Thus, a brief literature review of such studies enables that knowledge to reach international scholars and…

  1. Teaching Writing to Middle School Students in Portugal and in Brazil: An Exploratory Study

    ERIC Educational Resources Information Center

    Veiga Simão, Ana Margarida; Malpique, Anabela Abreu; Frison, Lourdes Maria Bragagnolo; Marques, André

    2016-01-01

    Learning how to write is a challenging process, typically developed in schools. Teachers' practices in teaching writing, however, have been under researched. The aim of this study was to survey a sample of teachers from Portugal (n = 96) and Brazil (n = 99) about their practices for and perceptions about writing instruction. Teachers reported on…

  2. Epidemiology of Autism Spectrum Disorder in Portugal: Prevalence, Clinical Characterization, and Medical Conditions

    ERIC Educational Resources Information Center

    Oliveira, Guiomar; Ataide, Assuncao; Marques, Carla; Miguel, Teresa S.; Coutinho, Ana Margarida; Mota-Vieira, Luisa; Goncalves, Esmeralda; Lopes, Nazare Mendes; Rodrigues, Vitor; Carmona da Mota, Henrique; Vicente, Astrid Moura

    2007-01-01

    The objective of this study was to estimate the prevalence of autistic spectrum disorder (ASD) and identify its clinical characterization, and medical conditions in a paediatric population in Portugal. A school survey was conducted in elementary schools, targeting 332 808 school-aged children in the mainland and 10 910 in the Azores islands.…

  3. Emergy analysis of a silvo-pastoral system, a case study in southern Portugal

    EPA Science Inventory

    The Mediterranean silvo-pastoral system known as Montado, in Portugal, is a complex land use system composed of an open tree stratum in various densities and an herbaceous layer, used for livestock grazing. Livestock also profit from the acorns, and the grazing contributes to avo...

  4. Early Childhood Intervention in Portugal: An Overview Based on the Developmental Systems Model

    ERIC Educational Resources Information Center

    Pinto, Ana Isabel; Grande, Catarina; Aguiar, Cecilia; de Almeida, Isabel Chaves; Felgueiras, Isabel; Pimentel, Julia Serpa; Serrano, Ana Maria; Carvalho, Leonor; Brandao, Maria Teresa; Boavida, Tania; Santos, Paula; Lopes-dos-Santos, Pedro

    2012-01-01

    Research studies on early childhood intervention (ECI) in Portugal are diffuse regarding both program components and the geographical area under scrutiny. Since the 1990s, a growing body of knowledge and evidence in ECI is being gathered, based on postgraduate teaching, in-service training, and research. This article draws on the systems theory…

  5. The Induction and Mentoring of New Teachers in Portugal: Contradictions, Needs and Opportunities

    ERIC Educational Resources Information Center

    Flores, Maria Assuncao; Ferreira, Fernando Ilidio

    2009-01-01

    In this article, policy and research on teacher induction in Portugal are examined. The lack of a formal induction system (despite recognition of its relevance in legal policy documents) and the mismatch between national regulations and practice are also discussed. The authors argue the need for a coherent and sustained induction and support…

  6. Higher Education Distance Learning in Portugal--State of the Art and Current Policy Issues

    ERIC Educational Resources Information Center

    Hasan, Abrar; Laaser, Wolfram

    2010-01-01

    The paper is based on a recent report given to the Portuguese Ministry of Science, Technology and Higher Education on "Reforming Higher Education Distance Learning in Portugal" (Hasan et. al. 2009). The report was grounded on on-site visits of leading Portuguese Universities, Polytechnics and Business Schools as well as on written statements of…

  7. European Social Fund in Portugal: A Complex Question for Human Resource Development

    ERIC Educational Resources Information Center

    Tome, Eduardo

    2012-01-01

    Purpose: This article aims to review the application of the funds awarded by the European Social Fund (ESF) to Portugal, since 1986, from a human resource development (HRD) perspective. Design/methodology/approach: Several variables are analyzed: investment, absorption, people, impact of investment, evolution of skills, main programs, supply and…

  8. Employment and Training by Sector in Greece, Spain and Portugal. CEDEFOP Flash 9/89.

    ERIC Educational Resources Information Center

    CEDEFOP Flash, 1989

    1989-01-01

    This publication of the European Centre for the Development of Vocational Training (CEDEFOP) describes a project investigating employment and training in Greece, Spain, and Portugal. An introduction tells why CEDEFOP has given priority to those countries. The second section reports the project's examination of the textiles and ready-to-wear…

  9. Seroprevalence of circulating Angiostrongylus vasorum antigen and parasite-specific antibodies in dogs from Portugal.

    PubMed

    Alho, Ana Margarida; Schnyder, Manuela; Schaper, Roland; Meireles, José; Belo, Silvana; Deplazes, Peter; de Carvalho, Luís Madeira

    2016-07-01

    Angiostrongylus vasorum is a nematode that lives in the pulmonary arteries and right cardiac ventricle of domestic dogs and wild canids. It is increasingly being reported in several European countries and North America. This parasite induces inflammatory verminous pneumonia, causing severe respiratory disease in dogs. In some instances, coagulopathies, neurological signs and even death may occur. Scant data are available regarding the occurrence of A. vasorum in Portugal. Therefore, sera of 906 shelter dogs from North to South mainland Portugal were collected. ELISAs to detect A. vasorum circulating antigen and specific antibodies against this parasite were performed. A total of six dogs [0.66 %, 95 % confidence intervals (CI) 0.24-1.43] were positive for both A. vasorum antigen and antibody detection, indicating an active infection, and 12 dogs (1.32 %, CI 0.68-2.30) were A. vasorum antibody-positive only. Regions with antigen- and antibody-positive animals overlapped and were distributed over nearly all sampled areas in the country. This is the first large-scale ELISA-based serological survey for A. vasorum in dogs from Portugal. The endemic occurrence of A. vasorum in dogs from different geographical areas of Portugal is therefore confirmed. PMID:27000086

  10. Comparing Higher Education Reforms in Finland and Portugal: Different Contexts, Same Solutions?

    ERIC Educational Resources Information Center

    Kauko, Jaakko; Diogo, Sara

    2012-01-01

    This article provides a comparative analysis of recent governance reforms in both Finnish and Portuguese higher education institutions (HEIs), following the OECD's recent reviews of both countries' tertiary education systems. While in the case of Finland the major problem was identified as being a lack of entrepreneurialism, Portugal was…