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Sample records for etici della ricerca

  1. Binding of GID1 to DELLAs promotes dissociation of GAF1 from DELLA in GA dependent manner

    PubMed Central

    Fukazawa, Jutarou; Ito, Takeshi; Kamiya, Yuji; Yamaguchi, Shinjiro; Takahashi, Yohsuke

    2015-01-01

    Gibberellins (GAs) are important phytohormones for plant growth and development. DELLAs are members of the plant-specific GRAS protein family and act as repressors of GA signaling. DELLAs are rapidly degraded in the presence of GAs. GA-GID1-DELLA complexes are recognized and ubiquitinated by the SCFSLY complex. The sleepy1 (sly1) F-box mutant exhibits dwarfism and low-germination phenotypes due to high accumulation of DELLAs. Overexpression of GID1 in the sly1 mutant partially rescues these phenotypes without degradation of DELLAs suggesting that proteolysis independent regulation of DELLAs exists in GA signaling. But the molecular mechanisms of non-proteolytic regulation of DELLA are largely unknown. Recently we identified a DELLA binding transcription factor, GAI-ASSOCIATED FACTOR1 (GAF1). GAF1 also interacts with co-repressor TOPLESS RELATED (TPR) in nuclei. DELLAs and TPR act as coactivator and corepressor of GAF1, respectively. GAs converts the GAF1 complex from transcriptional activator to repressor via degradation of DELLAs. The overexpression of ΔPAM, lacking of DELLAs binding region of GAF1, partially rescue dwarf phenotypes of GA deficient or GA insensitive mutant. In this study, we investigate the relationship between non-proteolytic regulation of DELLAs and GA signaling via DELLA-GAF1 complex using modified yeast two-hybrid system. PMID:26237582

  2. Fruit growth in Arabidopsis occurs via DELLA-dependent and DELLA-independent gibberellin responses.

    PubMed

    Fuentes, Sara; Ljung, Karin; Sorefan, Karim; Alvey, Elizabeth; Harberd, Nicholas P; Østergaard, Lars

    2012-10-01

    Fruit growth and development depend on highly coordinated hormonal activities. The phytohormone gibberellin (GA) promotes growth by inducing degradation of the growth-repressing DELLA proteins; however, the extent to which DELLA proteins contribute to GA-mediated gynoecium and fruit development remains to be clarified. Here, we provide an in-depth characterization of the role of DELLA proteins in Arabidopsis thaliana fruit growth. We show that DELLA proteins are key regulators of reproductive organ size and important for ensuring optimal fertilization. We demonstrate that the seedless fruit growth (parthenocarpy) observed in della mutants can be directly attributed to the constitutive activation of GA signaling. It has been known for >75 years that another hormone, auxin, can induce formation of seedless fruits. Using mutants with complete lack of DELLA activity, we show here that auxin-induced parthenocarpy occurs entirely through GA signaling in Arabidopsis. Finally, we uncover the existence of a DELLA-independent GA response that promotes fruit growth. This response requires GIBBERELLIN-INSENSITIVE DWARF1-mediated GA perception and a functional 26S proteasome and involves the basic helix-loop-helix protein SPATULA as a key component. Taken together, our results describe additional complexities in GA signaling during fruit development, which may be particularly important to optimize the conditions for successful reproduction. PMID:23064323

  3. Silencing of DELLA induces facultative parthenocarpy in tomato fruits.

    PubMed

    Martí, Cristina; Orzáez, Diego; Ellul, Philippe; Moreno, Vicente; Carbonell, Juan; Granell, Antonio

    2007-12-01

    DELLA proteins are plant nuclear factors that restrain growth and proliferation in response to hormonal signals. The effects of the manipulation of the DELLA pathway in the making of a berry-like fruit were investigated. The expression of the Arabidopsis thaliana gain-of-function DELLA allele Atgai (del) in tomato (Solanum lycopersicum L.) produced partially sterile dwarf plants and compacted influorescences, as expected for a constitutively activated growth repressor. In contrast, antisense silencing of the single endogenous tomato DELLA gene homologue (SlDELLA) produced slender-like plants with elongated flower trusses. Interestingly, the depletion of SlDELLA in tomato was sufficient to overcome the growth arrest normally imposed on the ovary at anthesis, resulting in parthenocarpic fruits in the absence of pollination. Antisense SlDELLA-engineered fruits were smaller in size and elongated in shape compared with wild type. Cell number estimations showed that fruit set, resulting from reduced SlDELLA expression, arose from activated cell elongation at the longitudinal and lateral axes of the fruit pericarp, bypassing phase-II (post-pollination) cell divisions. Parthenocarpy caused by SlDELLA depletion is facultative, as hand pollination restored wild-type fruit phenotype. This indicates that fertilization-associated SlDELLA-independent signals are operational in ovary-fruit transitions. SlDELLA was also found to restrain growth in other reproductive structures, affecting style elongation, stylar hair primordial growth and stigma development. PMID:17883372

  4. Regulation of the gibberellin pathway by auxin and DELLA proteins.

    PubMed

    O'Neill, Damian P; Davidson, Sandra E; Clarke, Victoria C; Yamauchi, Yukika; Yamaguchi, Shinjiro; Kamiya, Yuji; Reid, James B; Ross, John J

    2010-10-01

    The synthesis and deactivation of bioactive gibberellins (GA) are regulated by auxin and by GA signalling. The effect of GA on its own pathway is mediated by DELLA proteins. Like auxin, the DELLAs promote GA synthesis and inhibit its deactivation. Here, we investigate the relationships between auxin and DELLA regulation of the GA pathway in stems, using a pea double mutant that is deficient in DELLA proteins. In general terms our results demonstrate that auxin and DELLAs independently regulate the GA pathway, contrary to some previous suggestions. The extent to which DELLA regulation was able to counteract the effects of auxin regulation varied from gene to gene. For Mendel's LE gene (PsGA3ox1) no counteraction was observed. However, for another synthesis gene, a GA 20-oxidase, the effect of auxin was weak and in WT plants appeared to be completely over-ridden by DELLA regulation. For a key GA deactivation (2-oxidase) gene, PsGA2ox1, the up-regulation induced by auxin deficiency was reduced to some extent by DELLA regulation. A second pea 2-oxidase gene, PsGA2ox2, was up-regulated by auxin, in a DELLA-independent manner. In Arabidopsis also, one 2-oxidase gene was down-regulated by auxin while another was up-regulated. Monitoring the metabolism pattern of GA(20) showed that in Arabidopsis, as in pea, auxin can promote the accumulation of bioactive GA. PMID:20706734

  5. DELLA proteins regulate arbuscule formation in arbuscular mycorrhizal symbiosis

    PubMed Central

    Floss, Daniela S.; Levy, Julien G.; Lévesque-Tremblay, Véronique; Pumplin, Nathan; Harrison, Maria J.

    2013-01-01

    Most flowering plants are able to form endosymbioses with arbuscular mycorrhizal fungi. In this mutualistic association, the fungus colonizes the root cortex and establishes elaborately branched hyphae, called arbuscules, within the cortical cells. Arbuscule development requires the cellular reorganization of both symbionts, and the resulting symbiotic interface functions in nutrient exchange. A plant symbiosis signaling pathway controls the development of the symbiosis. Several components of the pathway have been identified, but transcriptional regulators that control downstream pathways for arbuscule formation are still unknown. Here we show that DELLA proteins, which are repressors of gibberellic acid (GA) signaling and function at the nexus of several signaling pathways, are required for arbuscule formation. Arbuscule formation is severely impaired in a Medicago truncatula Mtdella1/Mtdella2 double mutant; GA treatment of wild-type roots phenocopies the della double mutant, and a dominant DELLA protein (della1-Δ18) enables arbuscule formation in the presence of GA. Ectopic expression of della1-Δ18 suggests that DELLA activity in the vascular tissue and endodermis is sufficient to enable arbuscule formation in the inner cortical cells. In addition, expression of della1-Δ18 restores arbuscule formation in the symbiosis signaling pathway mutant cyclops/ipd3, indicating an intersection between DELLA and symbiosis signaling for arbuscule formation. GA signaling also influences arbuscule formation in monocots, and a Green Revolution wheat variety carrying dominant DELLA alleles shows enhanced colonization but a limited growth response to arbuscular mycorrhizal symbiosis. PMID:24297892

  6. Removal of DELLA repression promotes leaf senescence in Arabidopsis.

    PubMed

    Chen, Mingxun; Maodzeka, Antony; Zhou, Longhua; Ali, Essa; Wang, Zhong; Jiang, Lixi

    2014-04-01

    Leaf senescence is an integrated response of leaf cells to developmental age and various internal and environmental signals. However, the role of gibberellins (GA) in leaf senescence is not clear. In the current study, we investigated the effect of DELLA on leaf senescence. Compared with the wild type (WT), leaf senescence occurred earlier in the mutant ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1 (abbreviated as Q-DELLA/ga1-3) whose DELLA repression was removed, whereas leaf senescence was retarded in the mutant ga1-3 whose GA biosynthesis was blocked and whose DELLA proteins accumulated abnormally. During leaf senescence, SAG12 and SAG29 were upregulated in Q-DELLA/ga1-3 and downregulated in ga1-3 plants. The Q-DELLA/ga1-3 senescent leaves contained more sugar but less chlorophyll and fatty acids (FAs) than those of ga1-3 and WT. Both absolute and relative contents of C18:3 in Q-DELLA/ga1-3 senescent leaves were lower compared with those of the WT and ga1-3 leaves. The genes regulating FA β-oxidation in Q-DELLA/ga1-3, such as KAT2, LACS6, LACS7, ACX1, ACX2 and MAP2, were significantly upregulated. The removal of DELLA repression highly upregulated certain genes on various hormone pathways, suggesting that GA signaling acts upstream of the jasmonic acid, salicylic acid, and ethylene pathways in regulating leaf senescence. PMID:24576761

  7. Fruit Growth in Arabidopsis Occurs via DELLA-Dependent and DELLA-Independent Gibberellin Responses[W][OA

    PubMed Central

    Fuentes, Sara; Ljung, Karin; Sorefan, Karim; Alvey, Elizabeth; Harberd, Nicholas P.; Østergaard, Lars

    2012-01-01

    Fruit growth and development depend on highly coordinated hormonal activities. The phytohormone gibberellin (GA) promotes growth by inducing degradation of the growth-repressing DELLA proteins; however, the extent to which DELLA proteins contribute to GA-mediated gynoecium and fruit development remains to be clarified. Here, we provide an in-depth characterization of the role of DELLA proteins in Arabidopsis thaliana fruit growth. We show that DELLA proteins are key regulators of reproductive organ size and important for ensuring optimal fertilization. We demonstrate that the seedless fruit growth (parthenocarpy) observed in della mutants can be directly attributed to the constitutive activation of GA signaling. It has been known for >75 years that another hormone, auxin, can induce formation of seedless fruits. Using mutants with complete lack of DELLA activity, we show here that auxin-induced parthenocarpy occurs entirely through GA signaling in Arabidopsis. Finally, we uncover the existence of a DELLA-independent GA response that promotes fruit growth. This response requires GIBBERELLIN-INSENSITIVE DWARF1–mediated GA perception and a functional 26S proteasome and involves the basic helix-loop-helix protein SPATULA as a key component. Taken together, our results describe additional complexities in GA signaling during fruit development, which may be particularly important to optimize the conditions for successful reproduction. PMID:23064323

  8. Lifting DELLA repression of Arabidopsis seed germination by nonproteolytic gibberellin signaling

    Technology Transfer Automated Retrieval System (TEKTRAN)

    DELLA repression of Arabidopsis seed germination can be lifted through the ubiquitin-proteasome pathway and proteolysis-independent GA signaling. GA-binding to the GID1 (GIBBERELLIN-INSENSITIVE DWARF1) GA receptors stimulates GID1-GA-DELLA complex formation which in turn triggers DELLA protein ubiq...

  9. DELLA-mediated PIF degradation contributes to coordination of light and gibberellin signalling in Arabidopsis

    PubMed Central

    Li, Kunlun; Yu, Renbo; Fan, Liu-Min; Wei, Ning; Chen, Haodong; Deng, Xing Wang

    2016-01-01

    Light and gibberellins (GAs) antagonistically regulate hypocotyl elongation in plants. It has been demonstrated that DELLAs, which are negative regulators of GA signalling, inhibit phytochrome-interacting factors 3 and 4 (PIF3 and PIF4) by sequestering their DNA-recognition domains. However, it is unclear whether there are other mechanisms of regulatory crosstalk between DELLAs and PIFs. Here, we demonstrate that DELLAs negatively regulate the abundance of four PIF proteins through the ubiquitin–proteasome system. Reduction of PIF3 protein abundance by DELLAs correlates closely with reduced hypocotyl elongation. Both sequestration and degradation of PIF3 by DELLAs contribute to a reduction in PIF3 binding to its target genes. Thus, we show that promotion of PIF degradation by DELLAs is required to coordinate light and GA signals, and the dual regulation of transcription factors by DELLAs by both sequestration and degradation may be a general mechanism. PMID:27282989

  10. The "Accademia della Crusca" in Italy: Past and Present

    ERIC Educational Resources Information Center

    Tosi, Arturo

    2011-01-01

    An informal organisation that is becoming quite influential in the spread of Italian in Italy, as well as abroad, is the "Accademia della Crusca" which began its activities during the Renaissance, under quite different auspices. Founded in Florence in 1582-1583, this Academy was inspired by the theories of Pietro Bembo (1470-1547), a Venetian who…

  11. A DELLA in Disguise: SPATULA Restrains the Growth of the Developing Arabidopsis Seedling[C][W

    PubMed Central

    Josse, Eve-Marie; Gan, Yinbo; Bou-Torrent, Jordi; Stewart, Kelly L.; Gilday, Alison D.; Jeffree, Christopher E.; Vaistij, Fabián E.; Martínez-García, Jaime F.; Nagy, Ferenc; Graham, Ian A.; Halliday, Karen J.

    2011-01-01

    The period following seedling emergence is a particularly vulnerable stage in the plant life cycle. In Arabidopsis thaliana, the phytochrome-interacting factor (PIF) subgroup of basic-helix-loop-helix transcription factors has a pivotal role in regulating growth during this early phase, integrating environmental and hormonal signals. We previously showed that SPATULA (SPT), a PIF homolog, regulates seed dormancy. In this article, we establish that unlike PIFs, which mainly promote hypocotyl elongation, SPT is a potent regulator of cotyledon expansion. Here, SPT acts in an analogous manner to the gibberellin-dependent DELLAs, REPRESSOR OF GA1-3 and GIBBERELLIC ACID INSENSITIVE, which restrain cotyledon expansion alongside SPT. However, although DELLAs are not required for SPT action, we demonstrate that SPT is subject to negative regulation by DELLAs. Cross-regulation of SPT by DELLAs ensures that SPT protein levels are limited when DELLAs are abundant but rise following DELLA depletion. This regulation provides a means to prevent excessive growth suppression that would result from the dual activity of SPT and DELLAs, yet maintain growth restraint under DELLA-depleted conditions. We present evidence that SPT and DELLAs regulate common gene targets and illustrate that the balance of SPT and DELLA action depends on light quality signals in the natural environment. PMID:21478445

  12. Molecular cloning, expression analysis and subcellular localization of four DELLA genes from hybrid poplar.

    PubMed

    Liu, Sian; Xuan, Lei; Xu, Li-An; Huang, Minren; Xu, Meng

    2016-01-01

    Gibberellic acid (GA) signaling regulates diverse aspects of plant growth and developmental processes. The DELLA repressors of GA signaling are named for an N-terminal conserved DELLA domain. In this study, four genes encoding DELLA proteins, PeRGA1, PeRGA2, PeGAI1 and PeGAI2, were isolated and characterized in poplar. A gene structural analysis revealed that the DELLA genes were all intron-free. Multiple protein sequence alignments revealed that these proteins contained seven highly conserved domains: the DELLA domain, the TVHYNP domain, leucine heptad repeat I (LHR I), the VHIID domain, leucine heptad repeat II (LHR II), the PFYRE domain, and the SAM domain. Temporal expression patterns of these genes were profiled during the adventitious root development of poplar. The four DELLA genes were expressed in root, stem and leaf in a dynamic manner. The subcellular localization demonstrated that these DELLA genes were mainly localized to the nucleus. These results suggest that the four DELLA genes may play diverse regulatory roles in the adventitious root, stem and leaf development of poplar, and contribute to improving our understanding of conserved and divergent aspects of DELLA proteins that restrain GA signaling in various species. PMID:27478746

  13. A Pivotal Role of DELLAs in Regulating Multiple Hormone Signals.

    PubMed

    Davière, Jean-Michel; Achard, Patrick

    2016-01-01

    Plant phenotypic plasticity is controlled by diverse hormone pathways, which integrate and convey information from multiple developmental and environmental signals. Moreover, in plants many processes such as growth, development, and defense are regulated in similar ways by multiple hormones. Among them, gibberellins (GAs) are phytohormones with pleiotropic actions, regulating various growth processes throughout the plant life cycle. Previous work has revealed extensive interplay between GAs and other hormones, but the molecular mechanism became apparent only recently. Molecular and physiological studies have demonstrated that DELLA proteins, considered as master negative regulators of GA signaling, integrate multiple hormone signaling pathways through physical interactions with transcription factors or regulatory proteins from different families. In this review, we summarize the latest progress in GA signaling and its direct crosstalk with the main phytohormone signaling, emphasizing the multifaceted role of DELLA proteins with key components of major hormone signaling pathways. PMID:26415696

  14. Auxin acts independently of DELLA proteins in regulating gibberellin levels.

    PubMed

    Reid, James B; Davidson, Sandra E; Ross, John J

    2011-03-01

    Shoot elongation is a vital process for plant development and productivity, in both ecological and economic contexts. Auxin and bioactive gibberellins (GAs), such as GA1, play critical roles in the control of elongation, along with environmental and endogenous factors, including other hormones such as the brassinosteroids. The effect of auxins, such as indole-3-acetic acid (IAA), is at least in part mediated by its effect on GA metabolism, since auxin up-regulates biosynthesis genes such as GA 3-oxidase and GA 20-oxidase and down regulates GA catabolism genes such as GA 2-oxidases, leading to elevated levels of bioactive GA 1. In our recent paper, we have provided evidence that this action of IAA is largely independent of DELLA proteins, the negative regulators of GA action, since the auxin effects are still present in the DELLA-deficient la cry-s genotype of pea. This was a crucial issue to resolve, since like auxin, the DELLAs also promote GA 1 synthesis and inhibit its deactivation. DELLAs are deactivated by GA, and thereby mediate a feedback system by which bioactive GA regulates its own level. However, our recent results, in themselves, do not show the generality of the auxin-GA relationship across species and phylogenetic groups or across different tissue types and responses. Further, they do not touch on the ecological benefits of the auxin-GA interaction. These issues are discussed below as well as the need for the development of suitable experimental systems to allow this process to be examined. PMID:21358281

  15. Proteolysis-independent down-regulation of DELLA repression by the gibberellin receptor GID1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper presents evidence for proteolysis-independent regulation of DELLA repression of gibberellin (GA) signaling in Arabidopsis. DELLA proteins are negative regulators of GA responses including seed germination, stem elongation, and fertility. GA can stimulate GA responses by causing proteolys...

  16. Uncovering DELLA-Independent Gibberellin Responses by Characterizing New Tomato procera Mutants

    PubMed Central

    Livne, Sivan; Lor, Vai S.; Nir, Ido; Eliaz, Natanella; Aharoni, Asaph; Olszewski, Neil E.; Eshed, Yuval; Weiss, David

    2015-01-01

    Gibberellin (GA) regulates plant development primarily by triggering the degradation/deactivation of the DELLA proteins. However, it remains unclear whether all GA responses are regulated by DELLAs. Tomato (Solanum lycopersicum) has a single DELLA gene named PROCERA (PRO), and its recessive pro allele exhibits constitutive GA activity but retains responsiveness to external GA. In the loss-of-function mutant proΔGRAS, all examined GA developmental responses were considerably enhanced relative to pro and a defect in seed desiccation tolerance was uncovered. As pro, but not proΔGRAS, elongation was promoted by GA treatment, pro may retain residual DELLA activity. In agreement with homeostatic feedback regulation of the GA biosynthetic pathway, we found that GA20oxidase1 expression was suppressed in proΔGRAS and was not affected by exogenous GA3. In contrast, expression of GA2oxidase4 was not affected by the elevated GA signaling in proΔGRAS but was strongly induced by exogenous GA3. Since a similar response was found in Arabidopsis thaliana plants with impaired activity of all five DELLA genes, we suggest that homeostatic GA responses are regulated by both DELLA-dependent and -independent pathways. Transcriptome analysis of GA-treated proΔGRAS leaves suggests that 5% of all GA-regulated genes in tomato are DELLA independent. PMID:26036254

  17. Spatial control of flowering by DELLA proteins in Arabidopsis thaliana.

    PubMed

    Galvão, Vinicius C; Horrer, Daniel; Küttner, Frank; Schmid, Markus

    2012-11-01

    The transition from vegetative to reproductive development is a central event in the plant life cycle. To time the induction of flowering correctly, plants integrate environmental and endogenous signals such as photoperiod, temperature and hormonal status. The hormone gibberellic acid (GA) has long been known to regulate flowering. However, the spatial contribution of GA signaling in flowering time control is poorly understood. Here we have analyzed the effect of tissue-specific misexpression of wild-type and GA-insensitive (dellaΔ17) DELLA proteins on the floral transition in Arabidopsis thaliana. We demonstrate that under long days, GA affects the floral transition by promoting the expression of flowering time integrator genes such as FLOWERING LOCUS T (FT) and TWIN SISTER OF FT (TSF) in leaves independently of CONSTANS (CO) and GIGANTEA (GI). In addition, GA signaling promotes flowering independently of photoperiod through the regulation of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes in both the leaves and at the shoot meristem. Our data suggest that GA regulates flowering by controlling the spatial expression of floral regulatory genes throughout the plant in a day-length-specific manner. PMID:22992955

  18. Genome Wide Binding Site Analysis Reveals Transcriptional Coactivation of Cytokinin-Responsive Genes by DELLA Proteins

    PubMed Central

    Marín-de la Rosa, Nora; Pfeiffer, Anne; Hill, Kristine; Locascio, Antonella; Bhalerao, Rishikesh P.; Miskolczi, Pal; Grønlund, Anne L.; Wanchoo-Kohli, Aakriti; Thomas, Stephen G.; Bennett, Malcolm J.; Lohmann, Jan U.; Blázquez, Miguel A.; Alabadí, David

    2015-01-01

    The ability of plants to provide a plastic response to environmental cues relies on the connectivity between signaling pathways. DELLA proteins act as hubs that relay environmental information to the multiple transcriptional circuits that control growth and development through physical interaction with transcription factors from different families. We have analyzed the presence of one DELLA protein at the Arabidopsis genome by chromatin immunoprecipitation coupled to large-scale sequencing and we find that it binds at the promoters of multiple genes. Enrichment analysis shows a strong preference for cis elements recognized by specific transcription factor families. In particular, we demonstrate that DELLA proteins are recruited by type-B ARABIDOPSIS RESPONSE REGULATORS (ARR) to the promoters of cytokinin-regulated genes, where they act as transcriptional co-activators. The biological relevance of this mechanism is underpinned by the necessity of simultaneous presence of DELLAs and ARRs to restrict root meristem growth and to promote photomorphogenesis. PMID:26134422

  19. DELLA proteins modulate Arabidopsis defences induced in response to caterpillar herbivory

    PubMed Central

    Bede, Jacqueline C.

    2014-01-01

    Upon insect herbivory, many plant species change the direction of metabolic flux from growth into defence. Two key pathways modulating these processes are the gibberellin (GA)/DELLA pathway and the jasmonate pathway. In this study, the effect of caterpillar herbivory on plant-induced responses was compared between wild-type Arabidopsis thaliana (L.) Heynh. and quad-della mutants that have constitutively elevated GA responses. The labial saliva (LS) of caterpillars of the beet armyworm, Spodoptera exigua, is known to influence induced plant defence responses. To determine the role of this herbivore cue in determining metabolic shifts, plants were subject to herbivory by caterpillars with intact or impaired LS secretions. In both wild-type and quad-della plants, a jasmonate burst is an early response to caterpillar herbivory. Negative growth regulator DELLA proteins are required for the LS-mediated suppression of hormone levels. Jasmonate-dependent marker genes are induced in response to herbivory independently of LS, with the exception of AtPDF1.2 that showed LS-dependent expression in the quad-della mutant. Early expression of the salicylic acid (SA)-marker gene, AtPR1, was not affected by herbivory which also reflected SA hormone levels; however, this gene showed LS-dependent expression in the quad-della mutant. DELLA proteins may positively regulate glucosinolate levels and suppress laccase-like multicopper oxidase activity in response to herbivory. The present results show a link between DELLA proteins and early, induced plant defences in response to insect herbivory; in particular, these proteins are necessary for caterpillar LS-associated attenuation of defence hormones. PMID:24399173

  20. The Conservation Intervention on the Porta della Mandorla

    NASA Astrophysics Data System (ADS)

    Siano, S.; Giusti, A.; Pinna, D.; Porcinai, S.; Giamello, M.; Sabatini, G.; Salimbeni, R.

    We investigated the conservation problems of the Porta della Mandorla, a masterpiece of the early Florentine Renaissance. The aim of the work was the statement of practicable cleaning protocols based on laser ablation. To this goal, a thorough petrographic characterisation of the state of conservation was performed, which addressed a set of laser cleaning tests by Q-switching and short free running Nd:YAG lasers. The main optimisation problem was the yellow-orange appearance associated with laser ablation by Q-switching lasers. Following the explanation of such a cleaning result, two practicable solutions based on suitable pulse duration or wavelength selections were successfully demonstrated then different intervention protocols were proposed.

  1. Arabidopsis DELLA Protein Degradation Is Controlled by a Type-One Protein Phosphatase, TOPP4

    PubMed Central

    Qin, Qianqian; Wang, Wei; Guo, Xiaola; Yue, Jing; Huang, Yan; Xu, Xiufei; Li, Jia; Hou, Suiwen

    2014-01-01

    Gibberellins (GAs) are a class of important phytohormones regulating a variety of physiological processes during normal plant growth and development. One of the major events during GA-mediated growth is the degradation of DELLA proteins, key negative regulators of GA signaling pathway. The stability of DELLA proteins is thought to be controlled by protein phosphorylation and dephosphorylation. Up to date, no phosphatase involved in this process has been identified. We have identified a dwarfed dominant-negative Arabidopsis mutant, named topp4-1. Reduced expression of TOPP4 using an artificial microRNA strategy also resulted in a dwarfed phenotype. Genetic and biochemical analyses indicated that TOPP4 regulates GA signal transduction mainly via promoting DELLA protein degradation. The severely dwarfed topp4-1 phenotypes were partially rescued by the DELLA deficient mutants rga-t2 and gai-t6, suggesting that the DELLA proteins RGA and GAI are required for the biological function of TOPP4. Both RGA and GAI were greatly accumulated in topp4-1 but significantly decreased in 35S-TOPP4 transgenic plants compared to wild-type plants. Further analyses demonstrated that TOPP4 is able to directly bind and dephosphorylate RGA and GAI, confirming that the TOPP4-controlled phosphorylation status of DELLAs is associated with their stability. These studies provide direct evidence for a crucial role of protein dephosphorylation mediated by TOPP4 in the GA signaling pathway. PMID:25010794

  2. DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways.

    PubMed

    Jin, Yue; Liu, Huan; Luo, Dexian; Yu, Nan; Dong, Wentao; Wang, Chao; Zhang, Xiaowei; Dai, Huiling; Yang, Jun; Wang, Ertao

    2016-01-01

    Legumes form symbiotic associations with either nitrogen-fixing bacteria or arbuscular mycorrhizal fungi. Formation of these two symbioses is regulated by a common set of signalling components that act downstream of recognition of rhizobia or mycorrhizae by host plants. Central to these pathways is the calcium and calmodulin-dependent protein kinase (CCaMK)-IPD3 complex which initiates nodule organogenesis following calcium oscillations in the host nucleus. However, downstream signalling events are not fully understood. Here we show that Medicago truncatula DELLA proteins, which are the central regulators of gibberellic acid signalling, positively regulate rhizobial symbiosis. Rhizobia colonization is impaired in della mutants and we provide evidence that DELLAs can promote CCaMK-IPD3 complex formation and increase the phosphorylation state of IPD3. DELLAs can also interact with NSP2-NSP1 and enhance the expression of Nod-factor-inducible genes in protoplasts. We show that DELLA is able to bridge a protein complex containing IPD3 and NSP2. Our results suggest a transcriptional framework for regulation of root nodule symbiosis. PMID:27514472

  3. DELLA-mediated gibberellin signalling regulates Nod factor signalling and rhizobial infection.

    PubMed

    Fonouni-Farde, Camille; Tan, Sovanna; Baudin, Maël; Brault, Mathias; Wen, Jiangqi; Mysore, Kirankumar S; Niebel, Andreas; Frugier, Florian; Diet, Anouck

    2016-01-01

    Legumes develop symbiotic interactions with rhizobial bacteria to form nitrogen-fixing nodules. Bacterial Nod factors (NFs) and plant regulatory pathways modulating NF signalling control rhizobial infections and nodulation efficiency. Here we show that gibberellin (GA) signalling mediated by DELLA proteins inhibits rhizobial infections and controls the NF induction of the infection marker ENOD11 in Medicago truncatula. Ectopic expression of a constitutively active DELLA protein in the epidermis is sufficient to promote ENOD11 expression in the absence of symbiotic signals. We show using heterologous systems that DELLA proteins can interact with the nodulation signalling pathway 2 (NSP2) and nuclear factor-YA1 (NF-YA1) transcription factors that are essential for the activation of NF responses. Furthermore, MtDELLA1 can bind the ERN1 (ERF required for nodulation 1) promoter and positively transactivate its expression. Overall, we propose that GA-dependent action of DELLA proteins may directly regulate the NSP1/NSP2 and NF-YA1 activation of ERN1 transcription to regulate rhizobial infections. PMID:27586842

  4. DELLA proteins regulate expression of a subset of AM symbiosis-induced genes in Medicago truncatula.

    PubMed

    Floss, Daniela S; Lévesque-Tremblay, Véronique; Park, Hee-Jin; Harrison, Maria J

    2016-04-01

    The majority of the vascular flowering plants form symbiotic associations with fungi from the phylum Glomeromycota through which both partners gain access to nutrients, either mineral nutrients in the case of the plant, or carbon, in the case of the fungus. (1) The association develops in the roots and requires substantial remodeling of the root cortical cells where branched fungal hyphae, called arbuscules, are housed in a new membrane-bound apoplastic compartment. (2) Nutrient exchange between the symbionts occurs over this interface and its development and maintenance is critical for symbiosis. Previously, we showed that DELLA proteins, which are well known as repressors of gibberellic acid signaling, also regulate development of AM symbiosis and are necessary to enable arbuscule development. (3) Furthermore, constitutive overexpression of a dominant DELLA protein (della1-Δ18) is sufficient to induce transcripts of several AM symbiosis-induced genes, even in the absence of the fungal symbiont. (4) Here we further extend this approach and identify AM symbiosis genes that respond transcriptionally to constitutive expression of a dominant DELLA protein and also genes that do respond to this treatment. Additionally, we demonstrate that DELLAs interact with REQUIRED FOR ARBUSCULE DEVELOPMENT 1 (RAD1) which further extends our knowledge of GRAS factor complexes that have the potential to regulate gene expression during AM symbiosis. PMID:26984507

  5. DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways

    PubMed Central

    Jin, Yue; Liu, Huan; Luo, Dexian; Yu, Nan; Dong, Wentao; Wang, Chao; Zhang, Xiaowei; Dai, Huiling; Yang, Jun; Wang, Ertao

    2016-01-01

    Legumes form symbiotic associations with either nitrogen-fixing bacteria or arbuscular mycorrhizal fungi. Formation of these two symbioses is regulated by a common set of signalling components that act downstream of recognition of rhizobia or mycorrhizae by host plants. Central to these pathways is the calcium and calmodulin-dependent protein kinase (CCaMK)–IPD3 complex which initiates nodule organogenesis following calcium oscillations in the host nucleus. However, downstream signalling events are not fully understood. Here we show that Medicago truncatula DELLA proteins, which are the central regulators of gibberellic acid signalling, positively regulate rhizobial symbiosis. Rhizobia colonization is impaired in della mutants and we provide evidence that DELLAs can promote CCaMK–IPD3 complex formation and increase the phosphorylation state of IPD3. DELLAs can also interact with NSP2–NSP1 and enhance the expression of Nod-factor-inducible genes in protoplasts. We show that DELLA is able to bridge a protein complex containing IPD3 and NSP2. Our results suggest a transcriptional framework for regulation of root nodule symbiosis. PMID:27514472

  6. Relieving DELLA Repression of Stem Elongation and Flowering, Evidence for a Proteolysis Independent Mechanism for GA signaling

    Technology Transfer Automated Retrieval System (TEKTRAN)

    GA stimulates germination, stem elongation, and flowering by lifting DELLA protein repression of these responses via both proteolysis dependent and independent pathways. There are five members of the DELLA protein family in Arabidopsis with partially overlapping functions. GA biosynthesis lifts DELL...

  7. Mysteries of attraction: Giovanni Pico della Mirandola, astrology and desire.

    PubMed

    Rutkin, H Darrel

    2010-06-01

    Although in his later years Giovanni Pico della Mirandola (1463-1494) vehemently rejected astrology, he earlier used it in a variety of ways, but primarily to provide further evidence for positions to which he had arrived by other means. One such early use appears in his commentary on his friend Girolamo Benivieni's love poetry, the Canzone d'amore, of 1486-1487. In the passages discussed here, Pico presents an intensive Platonic natural philosophical analysis based on a deep astrologically informed understanding of human nature as he attempts to explain a perennial question, namely, why one person is attracted to a certain person (or people), and another to others. I will place this discussion of the mysteries of attraction and desire in historical perspective by tracing Pico's changing relationship to astrology during the course of his short but passionate life, and in historiographic perspective by revising Frances Yates's still influential views concerning Pico's contribution to Renaissance thought and his relationship with Marsilio Ficino. PMID:20513623

  8. JUB1 suppresses Pseudomonas syringae-induced defense responses through accumulation of DELLA proteins.

    PubMed

    Shahnejat-Bushehri, Sara; Nobmann, Barbara; Devi Allu, Annapurna; Balazadeh, Salma

    2016-06-01

    Phytohormones act in concert to coordinate plant growth and the response to environmental cues. Gibberellins (GAs) are growth-promoting hormones that recently emerged as modulators of plant immune signaling. By regulating the stability of DELLA proteins, GAs intersect with the signaling pathways of the classical primary defense hormones, salicylic acid (SA) and jasmonic acid (JA), thereby altering the final outcome of the immune response. DELLA proteins confer resistance to necrotrophic pathogens by potentiating JA signaling and raise the susceptibility to biotrophic pathogens by attenuating the SA pathway. Here, we show that JUB1, a core element of the GA - brassinosteroid (BR) - DELLA regulatory module, functions as a negative regulator of defense responses against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and mediates the crosstalk between growth and immunity. PMID:27159137

  9. Electronic restoration of great works of art: color analysis and reconstruction of Piero della Francesca's frescoes

    NASA Astrophysics Data System (ADS)

    Schwartz, Lillian F.

    1994-05-01

    A computer was used to examine the surface colors and compositions of Piero della Francesca's frescoes The Resurrection of Christ, St. Julien, and a section from the panel La morte di Adamo from the Leggenda della Vera Croce to electronically analyze Piero's palettes, repair cracks in his works, replace damaged areas, and identify sections in which earlier restorers changed the original work. Electronic restoration now serves as a road map to art restorers, as well as provides the public with a closer representation of works as the artist originally intended. The analysis also reveals surprising new insights into Piero della Francesca's The Resurrection of Christ, including the thorn-based structure of a symbolic tree, and resolves a historical controversy.

  10. JUB1 suppresses Pseudomonas syringae-induced defense responses through accumulation of DELLA proteins

    PubMed Central

    Shahnejat-Bushehri, Sara; Nobmann, Barbara; Devi Allu, Annapurna; Balazadeh, Salma

    2016-01-01

    ABSTRACT Phytohormones act in concert to coordinate plant growth and the response to environmental cues. Gibberellins (GAs) are growth-promoting hormones that recently emerged as modulators of plant immune signaling. By regulating the stability of DELLA proteins, GAs intersect with the signaling pathways of the classical primary defense hormones, salicylic acid (SA) and jasmonic acid (JA), thereby altering the final outcome of the immune response. DELLA proteins confer resistance to necrotrophic pathogens by potentiating JA signaling and raise the susceptibility to biotrophic pathogens by attenuating the SA pathway. Here, we show that JUB1, a core element of the GA - brassinosteroid (BR) - DELLA regulatory module, functions as a negative regulator of defense responses against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and mediates the crosstalk between growth and immunity. PMID:27159137

  11. Piero della Francesca's Sky in The Dream of Constantine

    NASA Astrophysics Data System (ADS)

    Valerio, V.

    2011-06-01

    The recent restoration of the frescoes by Piero della Francesca in the Church of San Francesco in Arezzo has made to appear on the background of the scene of Constantine's dream a number of stars. They are clearly painted with the intention to illustrate a sort of "natural" sky. In 2001 Anna Maria Maetzke recognized in a group of stars the constellation of the Ursa Minor, but so far no further study has been carried on to find any relation between the painted and the true sky. In this paper I show the existence of more constellations in the fresco, which are hardly detectable due to the mirror representation of the starry sky. Such a mirror image, as the Universe was seen from the outside, has a Greek origin and this kind of representation was introduced in Western Europe not only in celestial globes but also in star maps. This discovery leads to consider that Piero had at his disposal either a globe or a map which he reproduced on the fresco. My hypothesis is that a star map might be supplied to Piero by the astronomer Regiomontanus who was in Italy since 1461 following the Cardinal Bessarion in his journey from Wien to Rome. In 1463, Cardinal Bessarion was named papal legate to Venice and in July of the same year he leaved Rome together with Regiomontanus to reach Ferrara and Venice. The road to Venice crossed Umbria nearby Sansepolcro, Piero's birthplace not far from Arezzo. The trip took more than two weeks due to a stop before crossing the Apennines because the plague in Ferrara. Bessarion and Regiomontanus might have met Piero who was painting the cycle of frescoes in Arezzo and supplied him with a star map. Unfortunately, due to the lack of the horizon and any right line in the scene it is not possible to detect the latitude of the place corresponding to the painted sky.

  12. O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis.

    PubMed

    Zentella, Rodolfo; Hu, Jianhong; Hsieh, Wen-Ping; Matsumoto, Peter A; Dawdy, Andrew; Barnhill, Benjamin; Oldenhof, Harriëtte; Hartweck, Lynn M; Maitra, Sushmit; Thomas, Stephen G; Cockrell, Shelley; Boyce, Michael; Shabanowitz, Jeffrey; Hunt, Donald F; Olszewski, Neil E; Sun, Tai-Ping

    2016-01-15

    The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling in response to developmental and environmental cues. DELLAs also play a central role in mediating cross-talk between GA and other signaling pathways via antagonistic direct interactions with key transcription factors. However, how these crucial protein-protein interactions can be dynamically regulated during plant development remains unclear. Here, we show that DELLAs are modified by the O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) SECRET AGENT (SEC) in Arabidopsis. O-GlcNAcylation of the DELLA protein REPRESSOR OF ga1-3 (RGA) inhibits RGA binding to four of its interactors-PHYTOCHROME-INTERACTING FACTOR3 (PIF3), PIF4, JASMONATE-ZIM DOMAIN1, and BRASSINAZOLE-RESISTANT1 (BZR1)-that are key regulators in light, jasmonate, and brassinosteroid signaling pathways, respectively. Consistent with this, the sec-null mutant displayed reduced responses to GA and brassinosteroid and showed decreased expression of several common target genes of DELLAs, BZR1, and PIFs. Our results reveal a direct role of OGT in repressing DELLA activity and indicate that O-GlcNAcylation of DELLAs provides a fine-tuning mechanism in coordinating multiple signaling activities during plant development. PMID:26773002

  13. O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis

    PubMed Central

    Zentella, Rodolfo; Hu, Jianhong; Hsieh, Wen-Ping; Matsumoto, Peter A.; Dawdy, Andrew; Barnhill, Benjamin; Oldenhof, Harriëtte; Hartweck, Lynn M.; Maitra, Sushmit; Thomas, Stephen G.; Cockrell, Shelley; Boyce, Michael; Shabanowitz, Jeffrey; Hunt, Donald F.; Olszewski, Neil E.; Sun, Tai-ping

    2016-01-01

    The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling in response to developmental and environmental cues. DELLAs also play a central role in mediating cross-talk between GA and other signaling pathways via antagonistic direct interactions with key transcription factors. However, how these crucial protein–protein interactions can be dynamically regulated during plant development remains unclear. Here, we show that DELLAs are modified by the O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) SECRET AGENT (SEC) in Arabidopsis. O-GlcNAcylation of the DELLA protein REPRESSOR OF ga1-3 (RGA) inhibits RGA binding to four of its interactors—PHYTOCHROME-INTERACTING FACTOR3 (PIF3), PIF4, JASMONATE-ZIM DOMAIN1, and BRASSINAZOLE-RESISTANT1 (BZR1)—that are key regulators in light, jasmonate, and brassinosteroid signaling pathways, respectively. Consistent with this, the sec-null mutant displayed reduced responses to GA and brassinosteroid and showed decreased expression of several common target genes of DELLAs, BZR1, and PIFs. Our results reveal a direct role of OGT in repressing DELLA activity and indicate that O-GlcNAcylation of DELLAs provides a fine-tuning mechanism in coordinating multiple signaling activities during plant development. PMID:26773002

  14. Arabidopsis MYC2 Interacts with DELLA Proteins in Regulating Sesquiterpene Synthase Gene Expression[W][OA

    PubMed Central

    Hong, Gao-Jie; Xue, Xue-Yi; Mao, Ying-Bo; Wang, Ling-Jian; Chen, Xiao-Ya

    2012-01-01

    Arabidopsis thaliana flowers emit volatile terpenes, which may function in plant–insect interactions. Here, we report that Arabidopsis MYC2, a basic helix-loop-helix transcription factor, directly binds to promoters of the sesquiterpene synthase genes TPS21 and TPS11 and activates their expression. Expression of TPS21 and TPS11 can be induced by the phytohormones gibberellin (GA) and jasmonate (JA), and both inductions require MYC2. The induction of TPS21 and TPS11 results in increased emission of sesquiterpene, especially (E)-β-caryophyllene. DELLAs, the GA signaling repressors, negatively affect sesquiterpene biosynthesis, as the sesquiterpene synthase genes were repressed in plants overaccumulating REPRESSOR OF GA1-3 (RGA), one of the Arabidopsis DELLAs, and upregulated in a penta DELLA-deficient mutant. Yeast two-hybrid and coimmunoprecipitation assays demonstrated that DELLAs, represented by RGA, directly interact with MYC2. In yeast cells, the N terminus of MYC2 was responsible for binding to RGA. MYC2 has been proposed as a major mediator of JA signaling and crosstalk with abscisic acid, ethylene, and light signaling pathways. Our results demonstrate that MYC2 is also connected to GA signaling in regulating a subset of genes. In Arabidopsis inflorescences, it integrates both GA and JA signals into transcriptional regulation of sesquiterpene synthase genes and promotes sesquiterpene production. PMID:22669881

  15. N-terminal domains of DELLA proteins are intrinsically unstructured in the absence of interaction with GID1/gibberellic acid receptors.

    PubMed

    Sun, Xiaolin; Jones, William T; Harvey, Dawn; Edwards, Patrick J B; Pascal, Steven M; Kirk, Christopher; Considine, Thérèse; Sheerin, David J; Rakonjac, Jasna; Oldfield, Christopher J; Xue, Bin; Dunker, A Keith; Uversky, Vladimir N

    2010-04-01

    The plant growth-repressing DELLA proteins (DELLAs) are known to represent a convergence point in integration of multiple developmental and environmental signals in planta, one of which is hormone gibberellic acid (GA). Binding of the liganded GA receptor (GID1/GA) to the N-terminal domain of DELLAs is required for GA-induced degradation of DELLAs via the ubiquitin-proteasome pathway, thus derepressing plant growth. However, the conformational changes of DELLAs upon binding to GID1/GA, which are the key to understanding the precise mechanism of GID1/GA-mediated degradation of DELLAs, remain unclear. Using biophysical, biochemical, and bioinformatics approaches, we demonstrated for the first time that the unbound N-terminal domains of DELLAs are intrinsically unstructured proteins under physiological conditions. Within the intrinsically disordered N-terminal domain of DELLAs, we have identified several molecular recognition features, sequences known to undergo disorder-to-order transitions upon binding to interacting proteins in intrinsically unstructured proteins. In accordance with the molecular recognition feature analyses, we have observed the binding-induced folding of N-terminal domains of DELLAs upon interaction with AtGID1/GA. Our results also indicate that DELLA proteins can be divided into two subgroups in terms of their molecular compactness and their interactions with monoclonal antibodies. PMID:20103592

  16. The altarpieces of Della Robbia atelier in Marche region: investigations on technology and provenance

    NASA Astrophysics Data System (ADS)

    Amadori, M. L.; Barcelli, S.; Barcaioni, S.; Bouquillon, A.; Padeletti, G.; Pallante, P.

    2013-12-01

    Dissemination of Della Robbia glazed terracotta in the Marche (Italy) region started from the third decade of the 16th century. Numerous altarpieces, some of which no longer exist, document this artistic production. The protagonists of this diffusion phase were two of Andrea Della Robbia's sons, Marco (Fra Mattia) and Francesco (Fra Ambrogio). This paper shows the results of the scientific investigations carried out on constitutive materials of different altarpieces located in South Marche belonging to the Fra Mattia's production: the Coronation of Virgin between Saints Rocco, Sebastian, Peter martyr and Antonio abbot, dated back to 1527-1530, located in the collegiate church of S. Maria Assunta in Montecassiano; the Annunciation, dated back to 1520, placed in the church of S. Maria del Soccorso in Arcevia; the fragmentary Crowned Madonna and saints altarpiece, probably realized after 1531, today preserved in Civic Museum of Ripatransone. The first altarpiece was made in Montecassiano using two different assembling or production techniques: the external part of the lunette and the pillar strips are made of glazed polychrome terracotta, while the altar step and the internal part are an interesting and uncommon example of polychrome painted terracotta. The provenance of the glazed Arcevia altarpiece is not clear yet: some historians hypothesize a local manufacture of Fra Mattia and some others a Roman or Florentine production. The remaining parts of Ripatransone altarpiece are partially glazed and partially not coated perhaps because they were unfinished and not yet painted. Clay body samples collected from the above mentioned altarpieces were investigated using different analytical techniques (OM, XRD, XRF, PIXE) to point out differences in chemical and mineralogical composition and to determine if the altarpieces were made by using local raw clay materials or other clays from Tuscany or Campania as in the Della Robbia previous production. A comparison has also been

  17. The Renaissance and the universal surgeon: Giovanni Andrea Della Croce, a master of traumatology.

    PubMed

    Di Matteo, Berardo; Tarabella, Vittorio; Filardo, Giuseppe; Viganò, Anna; Tomba, Patrizia; Marcacci, Maurilio

    2013-12-01

    All the medical knowledge of all time in one book, the universal and perfect manual for the Renaissance surgeon, and the man who wrote it. This paper depicts the life and works of Giovanni Andrea della Croce, a 16th Century physician and surgeon, who, endowed with true spirit of Renaissance humanism, wanted to teach and share all his medical knowledge through his opus magnum, titled "Universal Surgery Complete with All the Relevant Parts for the Optimum Surgeon". An extraordinary book which truly represents a defining moment and a founding stone for traumatology, written by a lesser known historical personality, but nonetheless the Renaissance Master of Traumatology. PMID:24173678

  18. Lungo le vie di pellegrinaggio: la lezione astronomica di Gerberto a San Michele della Chiusa

    NASA Astrophysics Data System (ADS)

    Paladino, Laura C.

    A pilgrim on the trails of the Earth: that's how Gerbertus appears to us in the years preceding his pontificate as well in those, brief and intense, in which he was Pope. An ardent pilgrim of faith moved at the same time by vast intellectual interests that allowed him to come into contact with the most important schools of the time, of any cultural tradition; a pilgrim full of love, willing to transmit the enormous heritage of faith and knowledge of which he was custodian. The presence of the illustrious Benedictine is not explicitly documented in the Abbey of San Michele della Chiusa, but the signs of its cultural and ideological influence are evident both in historical memory as in the artistical vestiges of the Sacra: we investigate here, this presence, authoritative, original and versatile, to outline another aspect, still little investigated, of the lesson of Gerbert, monk, scientist, teacher and pastor.

  19. La meridiana di Egnazio Danti nella Torre dei Venti in Vaticano: un'icona della riforma Gregoriana del calendario

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2014-05-01

    La Torre dei Venti domina l’angolo Sud Ovest del cortile della Pigna (nell'area dei Musei Vaticani), ed è inclusa negli ambienti dell'Archivio Segreto Vaticano. Non è aperta al pubblico, ma è universalmente nota per la fama che da oltre quattrocento anni la circonda, legata alle vicende della riforma Gregoriana del calendario. La meridiana tracciata da padre Egnazio Danti (1536-1586) nella torre dei Venti, fu visitata anche da Gregorio XIII, probabilmente il 21 marzo 1581 come suppone il padre Stein, per convincersi dell'anticipo ormai arrivato a dieci giorni dell'equinozio di primavera sulla data che il concilio di Nicea aveva fissato al 21 marzo per il computo pasquale. La ricognizione astrometrica del febbraio-marzo 2009 fatta dall'autore viene qui presentata.

  20. [Modello di Management (Mo.Ma) del paziente affetto da schizofrenia: controllo della crisi, mantenimento, prevenzione delle ricadute e recovery con gli antipsicotici LAI].

    PubMed

    Brugnoli, Roberto; Rapinesi, Chiara; Kotzalidis, Georgios D; Marcellusi, Andrea; Mennini, Francesco S; De Filippis, Sergio; Carrus, Dario; Ballerini, Andrea; Francomano, Antonio; Ducci, Giuseppe; Del Casale, Antonio; Girardi, Paolo

    2016-01-01

    RIASSUNTO. La schizofrenia colpisce circa l'1% della popolazione e rappresenta un grave disturbo mentale con un notevole impatto anche sul funzionamento sociale, lavorativo e sulle attività della vita quotidiana. Le persone con schizofrenia hanno un tasso di mortalità superiore di 2-2,5 rispetto a quello della popolazione generale. La non aderenza ai farmaci antipsicotici è uno dei più importanti fattori di rischio per le ricadute e le ospedalizzazioni, sia nei pazienti con disturbo cronico sia al primo episodio, e conseguentemente contribuisce all'aumento dei costi sanitari. Gli antipsicotici atipici LAI possono migliorare l'aderenza al trattamento contribuendo a diminuire i tassi di ricaduta nei pazienti affetti da schizofrenia fin dall'esordio. Gli obiettivi primari nella gestione dei pazienti schizofrenici sono diretti, non solo alla riduzione dei sintomi nel breve termine, ma anche al mantenimento fisico e della funzionalità mentale, migliorando la qualità della vita e promuovendo il recupero del paziente. Scopo. Proporre un modello integrato, basato sulle evidenze, che fornisca un algoritmo efficace per il recupero del paziente schizofrenico e indagare l'efficacia e la sicurezza degli antipsicotici LAI nel trattamento, nel mantenimento, nella prevenzione delle ricadute e nella recovery dei pazienti affetti da schizofrenia. Metodi. Dopo un'accurata analisi di letteratura abbiamo identificato, raccolto e analizzato gli elementi qualificanti per un'ottimale gestione del paziente schizofrenico, definendo un modello di gestione e selezione delle alternative terapeutiche. Conclusioni. Il modello di gestione della schizofrenia proposto potrebbe consentire un migliore recupero funzionale del paziente grazie alla scelta di iniziare il percorso terapeutico, fin dalle prime fasi del disturbo soprattutto se in giovane età e al primo episodio, con un farmaco atipico LAI. All'interno dei farmaci antipsicotici atipici la formulazione LAI rappresenta una scelta

  1. The Arabidopsis DELLA RGA-LIKE3 Is a Direct Target of MYC2 and Modulates Jasmonate Signaling Responses[C][W

    PubMed Central

    Wild, Michael; Davière, Jean-Michel; Cheminant, Soizic; Regnault, Thomas; Baumberger, Nicolas; Heintz, Dimitri; Baltz, Rachel; Genschik, Pascal; Achard, Patrick

    2012-01-01

    Gibberellins (GAs) are plant hormones involved in the regulation of plant growth in response to endogenous and environmental signals. GA promotes growth by stimulating the degradation of nuclear growth–repressing DELLA proteins. In Arabidopsis thaliana, DELLAs consist of a small family of five proteins that display distinct but also overlapping functions in repressing GA responses. This study reveals that DELLA RGA-LIKE3 (RGL3) protein is essential to fully enhance the jasmonate (JA)-mediated responses. We show that JA rapidly induces RGL3 expression in a CORONATINE INSENSITIVE1 (COI1)– and JASMONATE INSENSITIVE1 (JIN1/MYC2)–dependent manner. In addition, we demonstrate that MYC2 binds directly to RGL3 promoter. Furthermore, we show that RGL3 (like the other DELLAs) interacts with JA ZIM-domain (JAZ) proteins, key repressors of JA signaling. These findings suggest that JA/MYC2-dependent accumulation of RGL3 represses JAZ activity, which in turn enhances the expression of JA-responsive genes. Accordingly, we show that induction of primary JA-responsive genes is reduced in the rgl3-5 mutant and enhanced in transgenic lines overexpressing RGL3. Hence, RGL3 positively regulates JA-mediated resistance to the necrotroph Botrytis cinerea and susceptibility to the hemibiotroph Pseudomonas syringae. We propose that JA-mediated induction of RGL3 expression is of adaptive significance and might represent a recent functional diversification of the DELLAs. PMID:22892320

  2. A CCaMK-CYCLOPS-DELLA Complex Activates Transcription of RAM1 to Regulate Arbuscule Branching.

    PubMed

    Pimprikar, Priya; Carbonnel, Samy; Paries, Michael; Katzer, Katja; Klingl, Verena; Bohmer, Monica J; Karl, Leonhard; Floss, Daniela S; Harrison, Maria J; Parniske, Martin; Gutjahr, Caroline

    2016-04-25

    Intracellular arbuscular mycorrhiza symbiosis between plants and glomeromycotan fungi leads to formation of highly branched fungal arbuscules that release mineral nutrients to the plant host. Their development is regulated in plants by a mechanistically unresolved interplay between symbiosis, nutrient, and hormone (gibberellin) signaling. Using a positional cloning strategy and a retrotransposon insertion line, we identify two novel alleles of Lotus japonicus REDUCED ARBUSCULAR MYCORRHIZA1 (RAM1) encoding a GRAS protein. We confirm that RAM1 is a central regulator of arbuscule development: arbuscule branching is arrested in L. japonicus ram1 mutants, and ectopic expression of RAM1 activates genes critical for arbuscule development in the absence of fungal symbionts. Epistasis analysis places RAM1 downstream of CCaMK, CYCLOPS, and DELLA because ectopic expression of RAM1 restores arbuscule formation in cyclops mutants and in the presence of suppressive gibberellin. The corresponding proteins form a complex that activates RAM1 expression via binding of CYCLOPS to a cis element in the RAM1 promoter. We thus reveal a transcriptional cascade in arbuscule development that employs the promoter of RAM1 as integrator of symbiotic (transmitted via CCaMK and CYCLOPS) and hormonal (gibberellin) signals. PMID:27020747

  3. A Gibberellin-Mediated DELLA-NAC Signaling Cascade Regulates Cellulose Synthesis in Rice[OPEN

    PubMed Central

    Huang, Debao; Wang, Shaogan; Zhang, Baocai; Shang-Guan, Keke; Shi, Yanyun; Zhang, Dongmei; Liu, Xiangling; Wu, Kun; Xu, Zuopeng; Fu, Xiangdong; Zhou, Yihua

    2015-01-01

    Cellulose, which can be converted into numerous industrial products, has important impacts on the global economy. It has long been known that cellulose synthesis in plants is tightly regulated by various phytohormones. However, the underlying mechanism of cellulose synthesis regulation remains elusive. Here, we show that in rice (Oryza sativa), gibberellin (GA) signals promote cellulose synthesis by relieving the interaction between SLENDER RICE1 (SLR1), a DELLA repressor of GA signaling, and NACs, the top-layer transcription factors for secondary wall formation. Mutations in GA-related genes and physiological treatments altered the transcription of CELLULOSE SYNTHASE genes (CESAs) and the cellulose level. Multiple experiments demonstrated that transcription factors NAC29/31 and MYB61 are CESA regulators in rice; NAC29/31 directly regulates MYB61, which in turn activates CESA expression. This hierarchical regulation pathway is blocked by SLR1-NAC29/31 interactions. Based on the results of anatomical analysis and GA content examination in developing rice internodes, this signaling cascade was found to be modulated by varied endogenous GA levels and to be required for internode development. Genetic and gene expression analyses were further performed in Arabidopsis thaliana GA-related mutants. Altogether, our findings reveal a conserved mechanism by which GA regulates secondary wall cellulose synthesis in land plants and provide a strategy for manipulating cellulose production and plant growth. PMID:26002868

  4. The Book of the Sick of Santa Maria della Morte in Bologna and the Medical Organization of a Hospital in the Sixteenth-Century.

    PubMed

    Savoia, Paolo

    2016-01-01

    In 2012 a manuscript was rediscovered in the Biblioteca dell'Archiginnasio of Bologna, titled Libro degli infermi dell'Arciconfraternita di S. Maria della Morte. It is the record of incoming patients of one for the main hospitals of the city, devoted exclusively to the sick poor and not just to the poor, called Santa Maria della Morte, compiled by a young student assistant (astante) for the period 1558-1564. I publish here a transcription of a portion of this Libro pertaining to the year 1560. My introduction situates the manuscript within the context of the history of early modern Italian hospitals, describes the organization of the hospital of Santa Maria della Morte based on archival sources of the period, and finally highlights the connections between surgical and anatomical education and the internal organization of the hospital. PMID:27071302

  5. The Megalithic Complex of the ``Preta 'ru Mulacchio'' on the Monte della Stella

    NASA Astrophysics Data System (ADS)

    Polcaro, V. F.; Ienna, D.

    2009-08-01

    The Monte della Stella is a 1131~m high mountain, belonging to the range separating the Alento Valley from the Tyrrhenian Sea, south of the city of Agropoli in Italy. At 1030~m over the sea-level, a large, isolated outcrop of the bedrock is present. This rock is well known to local people and called the ``Preta 'ru Mulacchio'', expression meaning in the local dialect ``The Bastard Child Rock''. The ``Preta'' is basically composed by three rocks that were originated along of natural reasons from a single block of arenite in its upper part and of a rough conglomerate in the lower one: between the three rocks, two galleries (thereafter F and G) were thus formed. However, it is easy to see that the ``Preta'' was deeply modified by human intervention: large stones were wedged in exact position between the three original blocks or positioned as a cover. We found that F gallery has an astronomical azimuth of 359 deg and G gallery of 240 deg. Inside the measurement precision (1 deg), the galleries are thus respectively oriented to the meridian and to the sunset of the winter solstice. Furthermore, modern folklore associated to the rock seems to remind very ancient fertility rites. From a statistical analysis of the alignments and an archaeological study of the complex, we conclude that ``Preta 'ru Mulacchio'' is most probably a monument, dated to an epoch presently unknown but possibly preceding the Greek colonization of Cilento, built in order to determine with a high precision the winter solstice because of cerimonial reasons, probably connected with fertility rites.

  6. Geometric morphometric methods for bone reconstruction: the mandibular condylar process of Pico della Mirandola.

    PubMed

    Benazzi, Stefano; Stansfield, Ekaterina; Kullmer, Ottmar; Fiorenza, Luca; Gruppioni, Giorgio

    2009-08-01

    The issue of reconstructing lost or deformed bone presents an equal challenge in the fields of paleoanthropology, bioarchaeology, forensics, and medicine. Particularly, within the disciplines of orthodontics and surgery, the main goal of reconstruction is to restore or create ex novo the proper form and function. The reconstruction of the mandibular condyle requires restoration of articulation, occlusion, and mastication from the functional side as well as the correct shape of the mandible from the esthetic point of view. Meeting all these demands is still problematic for surgeons. It is unfortunate that the collaboration between anthropologists and medical professionals is still limited. Nowadays, geometric morphometric methods (GMM) are routinely applied in shape analysis and increasingly in the reconstruction of missing data in skeletal material in paleoanthropology. Together with methods for three-dimensional (3D) digital model construction and reverse engineering, these methods could prove to be useful in surgical fields for virtual planning of operations and the production of customized biocompatible scaffolds. In this contribution, we have reconstructed the missing left condylar process of the mandible belonging to a famous Italian humanist of the 15th century, Pico della Mirandola (1463-1494) by means of 3D digital models and GMM, having first compared two methods (a simple reflection of the opposite side and the mathematical-statistical GMM approach) in a complete human mandible on which loss of the left condyle was virtually simulated. Finally, stereolithographic models of Pico's skull were prototyped providing the physical assembly of the bony skull structures with a high fitting accuracy. PMID:19645014

  7. A functional Small Ubiquitin-like Modifier (SUMO) interacting motif (SIM) in the gibberellin hormone receptor GID1 is conserved in cereal crops and disrupting this motif does not abolish hormone dependency of the DELLA-GID1 interaction

    PubMed Central

    Nelis, Stuart; Conti, Lucio; Zhang, Cunjin; Sadanandom, Ari

    2015-01-01

    Plants survive adversity by modulating their growth in response to changing environmental signals. The phytohormone Gibberellic acid (GA) plays a central role in regulating these adaptive responses by stimulating the degradation of growth repressing DELLA proteins which accumulate during stress. The current model for GA signaling describes how this hormone binds to its receptor GID1 so promoting association of GID1 with DELLA, which then undergoes ubiquitin-mediated proteasomal degradation. Recent data revealed that conjugation of DELLAs to the Small Ubiquitin-like Modifier (SUMO) protein enables plants to modulate its abundance during environmental stress. This is achieved by SUMOylated DELLAs sequestering GID1 via its SUMO interacting motif (SIM) allowing non-SUMOylated DELLAs to accumulate leading to growth restraint under stress and potential yield loss. We demonstrate that GID1 proteins across the major cereal crops contain a functional SIM able to bind SUMO1. Site directed mutagenesis and yeast 2 hybrid experiments reveal that it is possible to disrupt the SIM-SUMO interaction motif without affecting the GA dependent DELLA–GID1 interaction and thereby uncoupling SUMO–mediated inhibition from DELLA degradation. Arabidopsis plants overexpressing a SIM mutant allele of GID1 perform better at relieving DELLA restraint than wild–type GID1. This evidence suggests that manipulating the SIM motif in the GA receptor may provide a possible route to developing stress tolerant crops plants. PMID:25761145

  8. Procera is a putative DELLA mutant in tomato (Solanum lycopersicum): effects on the seed and vegetative plant.

    PubMed

    Bassel, George W; Mullen, Robert T; Bewley, J Derek

    2008-01-01

    The procera (pro) mutant of tomato exhibits a well-characterized constitutive gibberellic acid (GA) response phenotype. The tomato DELLA gene LeGAI in the pro mutant background contains a point mutation that results in an amino acid change in the conserved VHVID putative DNA-binding domain in LeGAI to VHEID. This same point mutation is in four different genetic backgrounds exhibiting the pro phenotype, suggesting that this mutation co-segregates with the pro phenotype. Complementation of the mutant with a constitutively expressed wild-type LeGAI gene sequence was not conclusive due to the infertility of transgenic plants. The pro mutation alters tomato branching architecture through differential suppression of axillary bud development, indicating a role for DELLA proteins in the regulation of plant structure. Isolated gib-1 pro double mutant embryo axes, which are unable to synthesize GA, germinate faster than their wild-type counterparts, and exert greater embryo growth potential. The pro mutation is therefore regulating GA responses within the tomato embryo. Transient expression of a LeGAI-GFP (green fluorescent protein) fusion protein in onion epidermis results in its location to the nucleus, and this protein is rapidly degraded by the proteasome in the presence of GA. PMID:18250077

  9. A Cucumber DELLA Homolog CsGAIP May Inhibit Staminate Development through Transcriptional Repression of B Class Floral Homeotic Genes

    PubMed Central

    Zhang, Yan; Liu, Bin; Yang, Sen; An, Jingbo; Chen, Chunhua; Zhang, Xiaolan; Ren, Huazhong

    2014-01-01

    In hermaphroditic Arabidopsis, the phytohormone gibberellin (GA) stimulates stamen development by opposing the DELLA repression of B and C classes of floral homeotic genes. GA can promote male flower formation in cucumber (Cucumis sativus L.), a typical monoecious vegetable with unisexual flowers, and the molecular mechanism remains unknown. Here we characterized a DELLA homolog CsGAIP in cucumber, and we found that CsGAIP is highly expressed in stem and male flower buds. In situ hybridization showed that CsGAIP is greatly enriched in the stamen primordia, especially during the hermaphrodite stage of flower development. Further, CsGAIP protein is located in nucleus. CsGAIP can partially rescue the plant height, stamen development and fertility phenotypes of Arabidopsis rga-24/gai-t6 mutant, and ectopic expression of CsGAIP in wide-type Arabidopsis results in reduced number of stamens and decreased transcription of B class floral homeotic genes APETALA3 (AP3) and PISTILLATA (PI). Our data suggest that monoecious CsGAIP may inhibit staminate development through transcriptional repression of B class floral homeotic genes in Arabidopsis. PMID:24632777

  10. Reconstruction of full glacial environments and summer temperatures from Lago della Costa, a refugial site in Northern Italy

    NASA Astrophysics Data System (ADS)

    Samartin, Stéphanie; Heiri, Oliver; Kaltenrieder, Petra; Kühl, Norbert; Tinner, Willy

    2016-07-01

    Vegetation and climate during the last ice age and the Last Glacial Maximum (LGM, ∼23,000-19,000 cal BP) were considerably different than during the current interglacial (Holocene). Cold climatic conditions and growing ice-sheets during the last glaciation radically reduced forest extent in Europe to a restricted number of so-called "refugia", mostly located in the southern part of the continent. On the basis of paleobotanical analyses the Euganian Hills (Colli Euganei) in northeastern Italy have previously been proposed as one of the northernmost refugia of temperate trees (e.g. deciduous Quercus, Tilia, Ulmus, Fraxinus excelsior, Acer, Abies alba, Fagus sylvatica, Carpinus and Castanea) in Europe. In this study we provide the first quantitative, vegetation independent summer air temperature reconstruction for Northern Italy spanning the time ∼31,000-17,000 cal yr BP, which covers the coldest periods of the last glacial, including the LGM and Heinrich stadials 1 to 3. Chironomids preserved in a lake sediment core from Lago della Costa (7m a.s.l.), a small lake at the south-eastern edge of the Euganean Hills, allowed quantitative reconstruction of Full and Late Glacial summer air temperatures using a combined Swiss-Norwegian temperature inference model based on chironomid assemblages from 274 lakes. Chironomid and pollen evidence from Lago della Costa derives from finely stratified autochthonous organic gyttja sediments, which excludes major sediment mixing or reworking. After reconstructing paleo-temperatures, we address the question whether climate conditions were warm enough to permit the local survival of temperate tree species during the LGM and whether local expansions and pollen-inferred contractions of temperate tree taxa coincided with chironomid-inferred climatic changes. Our results suggest that chironomids at Lago della Costa have responded to major climatic fluctuations such as temperature decreases during the LGM and Heinrich stadials. The

  11. The Montessori System of Education: An Examination of Characteristic Features Set Forth in Il Metodo Della Pedagogica Scientifica. Bulletin, 1912, No. 17. Whole Number 489

    ERIC Educational Resources Information Center

    Smith, Anna Tolman

    1912-01-01

    The publication of "Il metodo della pedagogica scientifica," by Dr. Maria Montessori, docent in the University of Rome, giving a full account of the inception and development of the system of education of which she is the author and the simultaneous translation of the work into English and German are events so unusual as to challenge attention.…

  12. "I CAMMINI DELLA REGINA" - Open Source based tools for preserving and culturally exploring historical traffic routes.

    NASA Astrophysics Data System (ADS)

    Cannata, Massimiliano; Colombo, Massimo; Antonovic, Milan; Cardoso, Mirko; Delucchi, Andrea; Gianocca, Giancarlo; Brovelli, Maria Antonia

    2015-04-01

    "I CAMMINI DELLA REGINA" (The Via Regina Paths) is an Interreg project funded within the transnational cooperation program between Italy and Switzerland 2007-2013. The aim of this project is the preservation and valorization of the cultural heritage linked to the walking historically paths crossing, connecting and serving the local territories. With the approach of leveraging the already existing tools, which generally consist of technical descriptions of the paths, the project uses the open source geospatial technologies to deploy innovative solutions which can fill some of the gaps in historical-cultural tourism offers. The Swiss part, and particularly the IST-SUPSI team, has been focusing its activities in the realization of two innovative solutions: a mobile application for the survey of historical paths and a storytelling system for immersive cultural exploration of the historical paths. The former, based on Android, allows to apply in a revised manner a consolidated and already successfully used methodology of survey focused on the conservation of the historical paths (Inventory of historical traffic routes in Switzerland). Up to now operators could rely only on hand work based on a combination of notes, pictures and GPS devices synthesized in manually drawn maps; this procedure is error prone and shows many problems both in data updating and extracting for elaborations. Thus it has been created an easy to use interface which allows to map, according to a newly developed spatially enabled data model, paths, morphological elements, and multimedia notes. When connected to the internet the application can send the data to a web service which, after applying linear referencing and further elaborating the data, makes them available using open standards. The storytelling system has been designed to provide users with cultural insights embedded in a multimedial and immersive geospatial portal. Whether the tourist is exploring physically or virtually the desired

  13. The growth history of the Lago Della Vacca (Southern Adamello Massive, Italy) intrusion from field observations, thermal and rheological modelling

    NASA Astrophysics Data System (ADS)

    Rust, A.; Annen, C.; Blundy, J. D.; Caricchi, L.

    2010-12-01

    The Lago Della Vacca granitoid is an intrusive body emplaced at about 4-6 km in up to 1 My. The core of the body is characterised by the presence of dyke-like structures, enclave-swarms and randomly distributed enclaves, which appear undeformed. Enclaves become oblate with the short axis perpendicular to the foliation, which, in turn follows the margin of the plutonic body (John and Blundy, 1993). Geothermometry and experimental data have been used to constrain the temperature of injection of the mafic component (1273-1323 K), the temperature of the host granitic magma (1173-1223), and to characterise the evolution of crystallinity with temperature for both magmas (Blundy and Sparks, 1992). Based on these data thermal and rheological modelling have been combined to interpret the growth and deformation history of the Lago della Vacca intrusive body. The pluton was modeled as a series of incrementally emplaced nested cylinders with 1D-cylindrical conductive heat transfer. The evolution of temperature and melt fraction distribution in the pluton and country rock were determined and used as input parameters for the rheological modelling. The rheology of each magma depends on the viscosity of the melt and, more importantly, on crystallinity. Field observations suggest that the mafic magma was injected as dykes. Their partial or total disaggregation produced mafic enclaves. The presence of randomly distributed enclaves in the core of Lago Della Vacca body indicates that convection was active in this portion of the intrusion. The undeformed nature of the enclaves in this region also implies that the contrast in temperature between host magma and mafic material produced a sudden (hours) rheological inversion with the mafic magma becoming more viscous than the felsic end-member. In these conditions, the enclaves would be transported passively by the felsic-host without suffering any substantial deformation. Thermal modelling indicates that to maintain the core of the pluton

  14. The Palazzo della Ragione in Padua: Representation and Communication of Art, Architecture, and Astrology of a Civic Monument

    NASA Astrophysics Data System (ADS)

    Borgherini, M.; Garbin, E.

    2011-06-01

    Eight centuries of the history of art and of Padua's scientific and technological culture deposited on the stones and frescoes of its Palace of Law ("Palazzo della Ragione") make this great work of urban architecture a part of the city's collective identity. This "palimpsest", legible only to a restricted circle of specialists, should be accessible to a vaster public interested in understanding this object symbol of local culture. The project planned for interactive exploration on the web is a series of digital models, employing tomographic-endoscopic visualizations and, in future, multi-resolution images. The various models devised allow the visitor to superimpose the Palace's current conditions on the various transformations undergone over the centuries. Similarly, comparisons can be made between the astrological fresco cycle with maps of the heavens, cosmological hypotheses, ancient and contemporary astrological treatises, and the related exchange of knowledge between the Orient and the Occident.

  15. Zoobotryon verticillatum Della Chiaje, 1822 (Bryozoa), a new occurrence in the archipelago of the Azores (North-Eastern Atlantic).

    PubMed

    Amat, Jaen Nieto; Tempera, Fernando

    2009-05-01

    The new occurrence of the bryozoan Zoobotryon verticillatum Della Chiaje, 1822 is herein recorded in multiple places throughout Azores archipelago. Where introduced, this species has caused important ecological and economical damage and therefore is regarded as invasive. In the Azores, no detrimental effects have yet been noted. The species is so far restricted to marinas (Horta, Faial Island; Vila Franca do Campo, São Miguel Island) and a natural coastal pool located near a secondary harbour (Lajes do Pico, Pico Island). A total of 29 colonies were counted during a specific survey conducted in August 2008 in the marina of Horta. The distribution of the species throughout the eastern and central island groups denotes a wide dispersion area and offers control or eradication measures a low probability of success together with the lack of harbour management practices that could effectively prevent the arrival, settlement and dispersal of non-native species transported by human-assisted means. PMID:19303606

  16. Molecular interactions of a soluble gibberellin receptor, GID1, with a rice DELLA protein, SLR1, and gibberellin.

    PubMed

    Ueguchi-Tanaka, Miyako; Nakajima, Masatoshi; Katoh, Etsuko; Ohmiya, Hiroko; Asano, Kenji; Saji, Shoko; Hongyu, Xiang; Ashikari, Motoyuki; Kitano, Hidemi; Yamaguchi, Isomaro; Matsuoka, Makoto

    2007-07-01

    GIBBERELLIN INSENSITIVE DWARF1 (GID1) encodes a soluble gibberellin (GA) receptor that shares sequence similarity with a hormone-sensitive lipase (HSL). Previously, a yeast two-hybrid (Y2H) assay revealed that the GID1-GA complex directly interacts with SLENDER RICE1 (SLR1), a DELLA repressor protein in GA signaling. Here, we demonstrated, by pull-down and bimolecular fluorescence complementation (BiFC) experiments, that the GA-dependent GID1-SLR1 interaction also occurs in planta. GA(4) was found to have the highest affinity to GID1 in Y2H assays and is the most effective form of GA in planta. Domain analyses of SLR1 using Y2H, gel filtration, and BiFC methods revealed that the DELLA and TVHYNP domains of SLR1 are required for the GID1-SLR1 interaction. To identify the important regions of GID1 for GA and SLR1 interactions, we used many different mutant versions of GID1, such as the spontaneous mutant GID1s, N- and C-terminal truncated GID1s, and mutagenized GID1 proteins with conserved amino acids replaced with Ala. The amino acid residues important for SLR1 interaction completely overlapped the residues required for GA binding that were scattered throughout the GID1 molecule. When we plotted these residues on the GID1 structure predicted by analogy with HSL tertiary structure, many residues were located at regions corresponding to the substrate binding pocket and lid. Furthermore, the GA-GID1 interaction was stabilized by SLR1. Based on these observations, we proposed a molecular model for interaction between GA, GID1, and SLR1. PMID:17644730

  17. Geophysic data interperetation of Passo della Morte landslide: Eastern Italian Alps

    NASA Astrophysics Data System (ADS)

    Zoppe', G.; Costa, G.; Marcato, G.; Forte, E.

    2012-04-01

    The Passo della Morte block-slide covers a relative large area in the Carnic Alps, along the left side of the Tagliamento River, between Forni di Sotto and Ampezzo (N-E of Italy). The high seismicity and the presence of the landslide increase the risk associated to the interest area. Moreover the large volume of material involved in the landslide (a few million of cubic meters), the presence of important infrastructure such as the road and two tunnels which cross the landslide, as well as the presence of the Tagliamento River that flow at the foot of the landslide, make the area very vulnerable. This study concerns with the western part of the Deep Seated Gravitational Slope Deformation (DSGSD). It focuses on the potential instability of a rock slope (crossed by road tunnels) and its connection with the DSGSD activity. The main objectives of this study are: monitoring the rock mass movement, studying the seismic site effect and defining the stratigraphic and geological characteristics of involved materials. Two vibration sensors have been installed inside the potential landslide: a short-period seismometer and a piezoelectric transducer. The microseismic activity recorded by the sensors has been analyzed, with particular regard to periods characterized by rapid changes in recorded seismic signals, and then correlated with the precipitation trend to evaluate the existence of a possible correlation between these phenomena. The microseismic activity study has highlighted the existence of a close link between microseisms and acoustic emissions recorded respectively by the seismometer and by the piezoelectric transducer. In addition, the comparison with the rainfall pattern has shown a direct relationship between different rainfall events and the sharp increase of microseismic activity detected by the two instruments. The correlation is good, even if acoustic emissions appear to be more sensitive than microseisms to short duration and low intensity rainfall events. The

  18. Human impact and Holocene climatic change in the archaeological site 'Piani della Corona

    NASA Astrophysics Data System (ADS)

    Pelle, Teresa; Scarciglia, Fabio; La Russa, Mauro F.; Natali, Elena; Tinè, Vincenzo

    2010-05-01

    A pedoarchaeological study was carried out in the archaeological site "Piani della Corona", located on a wide terrace at 500 m a.s.l. along the southwestern coast of Calabria, in southern Italy. The archaeological excavations exhumed an extensive settlement related to old to medium Bronze Age phases and traces of late Neolithic human colonization. On the basis of archaeological finds the pedostratigraphic succession can be partly dated. It consists of soils with variable features and andic properties, which include yellowish-brown (in places more reddish), deep argillic (Bt) horizons with variable amounts of clay coatings in pores and dark brown infillings of soil material rich in organic matter, in places overlaid by thin, severely truncated, brown to dark brown, organic-mineral (A) horizons. These layers include late Neolithic ceramic artefacts (Diana style facies) and typical incineration burials found in biconical vases, that can be referred to 6500-5000 years BP. The prehistoric layers are widely overlaid and strongly superimposed by a paleosurface of the early to medium Bronze age. This surface is affected by many pole holes left by large rectangular, apsidal wooden huts (not preserved), ploughed furrows, excavated cisterns, ditches and trenches, often filled by organic-rich dark brown material. Also hearths with charcoal remains, burials, vases and other diagnostic ceramic fragments occur. The upper portion of the pedostratigraphic succession consists of thicker brown A horizons, that appear cyclically ploughed during historical times (archaeologically not well dated as a consequence of their reworking for agricultural practices), with abrupt irregular boundaries often entering the underlying horizons. Micromorphological observations confirmed the presence of clay coatings within pores of Bt horizons, showing that they represent relict features (i.e. related to inactive illuvial processes, at present), as often fragmented and with smooth-banded to grainy

  19. Comparative Transcriptome Analysis of Genes Involved in GA-GID1-DELLA Regulatory Module in Symbiotic and Asymbiotic Seed Germination of Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae)

    PubMed Central

    Liu, Si-Si; Chen, Juan; Li, Shu-Chao; Zeng, Xu; Meng, Zhi-Xia; Guo, Shun-Xing

    2015-01-01

    Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae) is an endangered medicinal plant in China, also called “King Medicine”. Due to lacking of sufficient nutrients in dust-like seeds, orchid species depend on mycorrhizal fungi for seed germination in the wild. As part of a conservation plan for the species, research on seed germination is necessary. However, the molecular mechanism of seed germination and underlying orchid-fungus interactions during symbiotic germination are poorly understood. In this study, Illumina HiSeq 4000 transcriptome sequencing was performed to generate a substantial sequence dataset of germinating A. roxburghii seed. A mean of 44,214,845 clean reads were obtained from each sample. 173,781 unigenes with a mean length of 653 nt were obtained. A total of 51,514 (29.64%) sequences were annotated, among these, 49 unigenes encoding proteins involved in GA-GID1-DELLA regulatory module, including 31 unigenes involved in GA metabolism pathway, 5 unigenes encoding GID1, 11 unigenes for DELLA and 2 unigenes for GID2. A total of 11,881 genes showed significant differential expression in the symbiotic germinating seed sample compared with the asymbiotic germinating seed sample, of which six were involved in the GA-GID1-DELLA regulatory module, and suggested that they might be induced or suppressed by fungi. These results will help us understand better the molecular mechanism of orchid seed germination and orchid-fungus symbiosis. PMID:26694378

  20. Dominant and pleiotropic effects of a GAI gene in wheat results from a lack of interaction between DELLA and GID1.

    PubMed

    Wu, Jing; Kong, Xiuying; Wan, Jianmin; Liu, Xueying; Zhang, Xin; Guo, Xiuping; Zhou, Ronghua; Zhao, Guangyao; Jing, Ruilian; Fu, Xiangdong; Jia, Jizeng

    2011-12-01

    Dominance, semidominance, and recessiveness are important modes of Mendelian inheritance. The phytohormone gibberellin (GA) regulates many plant growth and developmental processes. The previously cloned semidominant GA-insensitive (GAI) genes Reduced height1 (Rht1) and Rht2 in wheat (Triticum aestivum) were the basis of the Green Revolution. However, no completely dominant GAI gene has been cloned. Here, we report the molecular characterization of Rht-B1c, a dominant GAI allele in wheat that confers more extreme characteristics than its incompletely dominant alleles. Rht-B1c is caused by a terminal repeat retrotransposons in miniature insertion in the DELLA domain. Yeast two-hybrid assays showed that Rht-B1c protein fails to interact with GA-INSENSITIVE DWARF1 (GID1), thereby blocking GA responses and resulting in extreme dwarfism and pleiotropic effects. By contrast, Rht-B1b protein only reduces interaction with GID1. Furthermore, we analyzed its functions using near-isogenic lines and examined its molecular mechanisms in transgenic rice. These results indicated that the affinity between GID1 and DELLA proteins is key to regulation of the stability of DELLA proteins, and differential interactions determine dominant and semidominant gene responses to GA. PMID:22010107

  1. Comparative Transcriptome Analysis of Genes Involved in GA-GID1-DELLA Regulatory Module in Symbiotic and Asymbiotic Seed Germination of Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae).

    PubMed

    Liu, Si-Si; Chen, Juan; Li, Shu-Chao; Zeng, Xu; Meng, Zhi-Xia; Guo, Shun-Xing

    2015-01-01

    Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae) is an endangered medicinal plant in China, also called "King Medicine". Due to lacking of sufficient nutrients in dust-like seeds, orchid species depend on mycorrhizal fungi for seed germination in the wild. As part of a conservation plan for the species, research on seed germination is necessary. However, the molecular mechanism of seed germination and underlying orchid-fungus interactions during symbiotic germination are poorly understood. In this study, Illumina HiSeq 4000 transcriptome sequencing was performed to generate a substantial sequence dataset of germinating A. roxburghii seed. A mean of 44,214,845 clean reads were obtained from each sample. 173,781 unigenes with a mean length of 653 nt were obtained. A total of 51,514 (29.64%) sequences were annotated, among these, 49 unigenes encoding proteins involved in GA-GID1-DELLA regulatory module, including 31 unigenes involved in GA metabolism pathway, 5 unigenes encoding GID1, 11 unigenes for DELLA and 2 unigenes for GID2. A total of 11,881 genes showed significant differential expression in the symbiotic germinating seed sample compared with the asymbiotic germinating seed sample, of which six were involved in the GA-GID1-DELLA regulatory module, and suggested that they might be induced or suppressed by fungi. These results will help us understand better the molecular mechanism of orchid seed germination and orchid-fungus symbiosis. PMID:26694378

  2. A scientific approach to the characterization of the painting materials of Fra Mattia della Robbia polychrome terracotta altarpiece

    NASA Astrophysics Data System (ADS)

    Amadori, M. L.; Barcelli, S.; Casoli, A.; Mazzeo, R.; Prati, S.

    2013-12-01

    During the last restoration (2008-2011) of the polychrome terracotta altarpiece called Coronation of Virgin between Saints Rocco, Sebastian, Peter martyr and Antonio abbot, located in the collegiate church of S. Maria Assunta in Montecassiano (Macerata, Italy), scientific investigations were carried out to acquire detailed information about the painting technique. The identification of materials allowed a correct restoration. The altarpiece is almost entirely realized by Marco della Robbia (Fra Mattia), dates back to the first half of the XVI century and represents an interesting example of painted terracotta produced by using two different techniques: glazed polychrome terracotta and the "cold painting" technique. The characterization of the samples' material constituents was obtained by analysing the cross-sections and the fragments by different techniques (optical, SEM-EDS and ATR-FTIR microscopy as well as GC-MS), as the real nature of a component is often difficult to assess with one single technique. The optical microscope examination of paint cross-sections shows the presence of many layers, indicating the complexity of the paint stratigraphic morphologies. The original polychromy of della Robbia's masterpiece is constituted of cinnabar, red lake, red lead, orpiment, red ochre, lead white, lead tin yellow, green earth and raw umber. Two different types of gilding technique have been distinguished. The first one presents a glue mordant, and the second one shows an oil mordant composed by a mixture of red lead, red ochre, cinnabar and orpiment. The GC-MS analysis allowed the characterisation of linseed oil and a mixture of animal glue and egg as binding media stratigraphically located by the use of ATR-FTIR mapping microscopy. The analytical results of the painted terracotta integrated investigations show that original technique adopted is characterised by the application of pigments in an oil-binding medium directly applied on the substrates, probably treated

  3. DELLA Proteins and Their Interacting RING Finger Proteins Repress Gibberellin Responses by Binding to the Promoters of a Subset of Gibberellin-Responsive Genes in Arabidopsis[C][W

    PubMed Central

    Park, Jeongmoo; Nguyen, Khoa Thi; Park, Eunae; Jeon, Jong-Seong; Choi, Giltsu

    2013-01-01

    DELLA proteins, consisting of GA INSENSITIVE, REPRESSOR OF GA1-3, RGA-LIKE1 (RGL1), RGL2, and RGL3, are central repressors of gibberellin (GA) responses, but their molecular functions are not fully understood. We isolated four DELLA-interacting RING domain proteins, previously designated as BOTRYTIS SUSCEPTIBLE1 INTERACTOR (BOI), BOI-RELATED GENE1 (BRG1), BRG2, and BRG3 (collectively referred to as BOIs). Single mutants of each BOI gene failed to significantly alter GA responses, but the boi quadruple mutant (boiQ) showed a higher seed germination frequency in the presence of paclobutrazol, precocious juvenile-to-adult phase transition, and early flowering, all of which are consistent with enhanced GA signaling. By contrast, BOI overexpression lines displayed phenotypes consistent with reduced GA signaling. Analysis of a gai-1 boiQ pentuple mutant further indicated that the GAI protein requires BOIs to inhibit a subset of GA responses. At the molecular level, BOIs did not significantly alter the stability of a DELLA protein. Instead, BOI and DELLA proteins are targeted to the promoters of a subset of GA-responsive genes and repress their expression. Taken together, our results indicate that the DELLA and BOI proteins inhibit GA responses by interacting with each other, binding to the same promoters of GA-responsive genes, and repressing these genes. PMID:23482857

  4. [Teoria della Mente e funzionamento sociale nella schizofrenia: correlazione con anomalie del linguaggio figurato, sintomatologia clinica e intelligenza generale].

    PubMed

    Piovan, Cristiano; Gava, Laura; Campeol, Mara

    2016-01-01

    RIASSUNTO. Scopo. Negli ultimi decenni gli studi hanno mostrato come la Teoria della Mente (ToM) non sia un processo unitario, ma un sistema che include aspetti cognitivi e affettivi. Nell'ambito dei domini che definiscono la social cognition, la ToM rappresenta il miglior predittore dello scarso funzionamento sociale nella schizofrenia. Lo scopo del presente lavoro è stato di esaminare la competenza di un gruppo di pazienti ambulatoriali affetti da schizofrenia in compiti di ToM, di riconoscimento di aspetti metaforici e idiomatici del linguaggio, in una prova di rispetto di regole conversazionali e di indagarne la relazione con il funzionamento sociale. Metodi. Sono stati reclutati 30 pazienti ambulatoriali con diagnosi di schizofrenia e 24 controlli sani. Sono stati somministrati il TIB per il calcolo del QI premorboso, la PANSS, il Theory of Mind Picture Sequencing Task, un test di comprensione di metafore e idiomi e un test conversazionale. Il funzionamento sociale è stato valutato con la PSP. Risultati. Non vi era differenza significativa tra i valori medi del QI premorboso del gruppo dei pazienti e dei controlli. Ai test di ToM e di competenza pragmatica, la differenza tra i gruppi è risultata altamente significativa, con i pazienti che hanno eseguito le prove in modo peggiore. È emersa una correlazione tra la comprensione di metafore e idiomi e le false credenze di secondo ordine. La PSP è risultata correlata con la PANSS e con la quota di ToM cognitiva, ma non con la ToM affettiva. Conclusioni. I risultati hanno mostrato che i soggetti affetti da schizofrenia, in condizione clinica di stabilizzazione, hanno evidenti difficoltà nelle prove di ToM e di comprensione del linguaggio figurato. Nel nostro modello teorico, la correlazione evidenziata tra la ToM cognitiva, i deficit pragmatici, lo stato clinico e il livello di funzionamento sociale suggerisce l'utilità di interventi riabilitativi di recupero delle funzioni metacognitive e delle abilit

  5. Reconstruction of full glacial environments and summer air temperatures from Lago della Costa, a refugial site in northeastern Italy.

    NASA Astrophysics Data System (ADS)

    Samartin, S. V.; Heiri, O.; Boltshauser-Kaltenrieder, P.; Tinner, W.

    2014-12-01

    Vegetation and climate during the Last Glacial Maximum (LGM) were considerably different than during the current interglacial (Holocene). In Europe large areas north of 40°N were entirely covered by continental ice-sheets and widespread permafrost, with temperatures around 10-20°C lower than at present, whereas further south aridity and temperatures 7-10°C cooler than today occurred. Cool climatic conditions and growing ice-sheets during the LGM radically reduced forest extent and diversity in Europe to a restricted number of so-called "refugia", mostly located in the southern part of the continent. The Euganian Hills in northeastern Italy are supposed to be one of the northernmost refugia of thermophilous mixed oak forest species (e.g. deciduous Quercus, Tilia, Ulmus, Fraxinus excelsior, Acer, Carpinus, Castanea) as well of some temperate mesophilous species (e.g. Fagus sylvatica, Abies alba) in Europe. In this study we present the first European chironomid-based quantitative temperature reconstruction for the LGM and address the question whether climate conditions were warm enough to permit the local survival of Quercetum mixtum species between ca. 31'000-17'000 cal yr BP. Chironomids preserved in a lake sediment core from Lago della Costa (7m a.s.l.), a lake on the border of the Euganean Hills in northeastern Italy, allowed quantitative reconstruction of Full and Late Glacial July air temperatures using a combined Swiss-Norwegian temperature inference model based on chironomid assemblages from 274 lakes. Our results suggest that July air temperatures never fell below 10°C which are considered necessary for forest growth. In general, mild climatic conditions prevailed between ca. 31'000-17'000 cal yr BP with temperatures ranging from ca. 11°C to 15.7°C. The expansion of thermophilous trees such as Quercus, Tilia, Ulmus, Fraxinus excelsior, Acer, Carpinus, Castanea (Quercetum mixtum) between ca. 30'000-23'000 cal yr BP can most likely be explained by climate

  6. A DELLA gene, RhGAI1, is a direct target of EIN3 and mediates ethylene-regulated rose petal cell expansion via repressing the expression of RhCesA2.

    PubMed

    Luo, Jing; Ma, Nan; Pei, Haixia; Chen, Jiwei; Li, Jing; Gao, Junping

    2013-11-01

    Ethylene plays an important role in organ growth. In Arabidopsis, ethylene can inhibit root elongation by stabilizing DELLA proteins. In previous work, it was found that ethylene suppressed cell expansion in rose petals, and five unisequences of DELLA genes are induced by ethylene. However, the mechanism of transcriptional regulation of DELLA genes by ethylene is still not clear. The results showed that the expression of RhGAI1 was induced in both ethylene-treated and ETR gene-silenced rose petals, and the promoter activity of RhGAI1 was strongly induced by RhEIN3-3 in Arabidopsis protoplasts. What is more, RhEIN3-3 could bind to the promoter of RhGAI1 directly in an electrophoretic mobility shift assay (EMSA). Cell expansion was suppressed in RhGAI1-Δ17-overexpressed Arabidopsis petals and promoted in RhGAI1-silenced rose petals. Moreover, in RhGAI1-silenced petals, the expression of nine cell expansion-related genes was clearly changed, and RhGAI1 can bind to the promoter of RhCesA2 in an EMSA. These results suggested that RhGAI1 was regulated by ethylene at the transcriptional level, and RhGAI1 was a direct target of RhEIN3-3. Also, RhGAI1 was shown to be involved in cell expansion partially through regulating the expression of cell expansion-related genes. Furthermore, RhCesA2 was a direct target of RhGAI1. This work uncovers the transcriptional regulation of RhGAI1 by ethylene and provides a better understanding of how ethylene regulates petal expansion in roses. PMID:24014864

  7. Integrated approach based on non-invasive investigations for the structural diagnosis of monuments: the case of the San Francesco della Scarpa Church in Lecce

    NASA Astrophysics Data System (ADS)

    Gizzi, Fabrizio; Leucci, Giovanni; Masini, Nicola; Persico, Raffaele; Quarta, Giovanni

    2015-04-01

    The paper shows the results of a diagnostics survey, based on the ground penetrating radar (GPR), seismic tomography and microtremor horizontal-to-vertical ratio (HVSR) method, to understand the causes of some static instability problems affecting the Church of San Francesco della Scarpa in Lecce (Apulia region, Southern Italy). The prospecting falls within the more general framework of a diagnostic investigation campaign for the restoration of the monument. This study case points out the great effectiveness of the employed diagnostic methods, when used in an integrated way, for detecting cracks and inhomogeneities in the inner structure of masonry building elements [1-2]. With regard to GPR prospecting, in order to better evidence the micro-fracture, a new algorithm, based on a clutter removal technique, has been used. In particular, it removes various unwanted signals such as cross talk, initial ground reflection and antenna ringing. Moreover, seismic tomographies provided complementary information on the mediocre state of conservation of some load bearing structures of the church. Finally, HVSR method allowed to study the relationship between decay patterns, instability problems and seismic response of the monument. Reference [1] Leucci G., Masini N., Persico R., Soldovieri F. 2011. GPR and sonic tomography for structural restoration: the case of the cathedral of Tricarico, Journal of Geophysics and Engineering, 8 (3), 76-92, doi:10.1088/1742-2132/8/3/S08. [2] Calia A., Leucci G., Masini N., Matera L., Persico R., Sileo M., 2012. Integrated prospecting in the Crypt of the Basilica of Saint Nicholas in Bari, Italy. Journal of Geophysics and Engineering, 9(3), 271-281, doi:10.1088/1742-2132/9/3/271.

  8. Knockdown of a JmjC domain-containing gene JMJ524 confers altered gibberellin responses by transcriptional regulation of GRAS protein lacking the DELLA domain genes in tomato

    PubMed Central

    Li, Jinhua; Yu, Chuying; Wu, Hua; Luo, Zhidan; Ouyang, Bo; Cui, Long; Zhang, Junhong; Ye, Zhibiao

    2015-01-01

    Plants integrate responses to independent hormonal and environmental signals to survive adversity. In particular, the phytohormone gibberellin (GA) regulates a variety of developmental processes and stress responses. In this study, the Jumonji-C (JmjC) domain-containing gene JMJ524 was characterized in tomato. JMJ524 responded to circadian rhythms and was upregulated by GA treatment. Knockdown of JMJ524 by RNAi caused a GA-insensitive dwarf phenotype with shrunken leaves and shortened internodes. However, in these transgenic plants, higher levels of endogenous GAs were detected. A genome-wide gene expression analysis by RNA-seq indicated that the expression levels of two DELLA-like genes, SlGLD1 (‘GRAS protein Lacking the DELLA domain’) and SlGLD2, were increased in JMJ524-RNAi transgenic plants. Nevertheless, only the overexpression of SlGLD1 in tomato resulted in a GA-insensitive dwarf phenotype, suggesting that SlGLD1 acts as a repressor of GA signalling. This study proposes that JMJ524 is required for stem elongation by altering GA responses, at least partially by regulating SlGLD1. PMID:25680796

  9. Application of some integrated non-invasive sensing techniqes for conservation and restoration of the Underground Church and frescoes of S. Maria della Palomba's Sanctuary, Matera (Italy)

    NASA Astrophysics Data System (ADS)

    Lembo, Filiberto; Marino, Francesco P.; Ambrosecchia, Nicola

    2010-05-01

    Santa Maria della Palomba's Sanctuary was built in XV century on a pre-existing medioeval crypt, in a splendid landscape situation, on the front looking south of the ravine on which stands Matera, integrating in a wonderful way underground and sub divo building; in fairly following time one important cycle of frescoes renewed decoration of underground church. In the long run, felt the building into decay, structures and frescoes were flooded and damaged; so that from 1980 were executed important restoration works, realizing ventilation canals under the floor of hypogeic church, in which were incorporated heating pipes, joined to solar thermic panels, wanting to determine the thermo-hygrometric optimum conditions for conservation. Almost thirty years after, willing restorate the frescoes, it was necessary to caracterize completely and objectively physical existing conditions. So was used an integrated mix of some non-invasive sensing techniques: - internal and external high resolution Sanctuary's measurement, using laser scanner 3D, in WebGIS ambient, so as to specify, in particular, whether dimensional data of non accessible parts (thickness of rock-bank, morphology and way of lying down of fracture lines), whether consistence state of frescoes; - thermo-hygrometrical sensing of surfaces, using infrared thermography, during a time of two weeks, in correspondence of many storm, so that to establish the relation between eventual atmospherical conditions variations and changes in conditions of surfaces; - continuous sensing of condition of surfaces, by means of thermo-hygrometrical and temperature sounds; - continuous sensing of operating temperature, by means of a globothermometer; all these tools were linked in a net with a data logger, and informations were transmitted using Web to computer in the office of the Society responsible for the procedure. Remote sensing integrated system proved high liability, allowing many important functions : - to georefer all data

  10. Riprese CCD della chioma interna della cometa Ikeya-Zhang

    NASA Astrophysics Data System (ADS)

    Comolli, Lorenzo

    2003-03-01

    During the period of maximum brightness of comet Ikeya-Zhang we acquired many unsaturated CCD images thanks to a period of good weather. The series of images of about 2 weeks can be animated so that changes in the inner coma are made evident. Elaborations with Larson-Sekanina filter and masking filters evidence details like concentric halos, jets and shells.

  11. Characterization of the procera Tomato Mutant Shows Novel Functions of the SlDELLA Protein in the Control of Flower Morphology, Cell Division and Expansion, and the Auxin-Signaling Pathway during Fruit-Set and Development1[C][W

    PubMed Central

    Carrera, Esther; Ruiz-Rivero, Omar; Peres, Lazaro Eustaquio Pereira; Atares, Alejandro; Garcia-Martinez, Jose Luis

    2012-01-01

    procera (pro) is a tall tomato (Solanum lycopersicum) mutant carrying a point mutation in the GRAS region of the gene encoding SlDELLA, a repressor in the gibberellin (GA) signaling pathway. Consistent with the SlDELLA loss of function, pro plants display a GA-constitutive response phenotype, mimicking wild-type plants treated with GA3. The ovaries from both nonemasculated and emasculated pro flowers had very strong parthenocarpic capacity, associated with enhanced growth of preanthesis ovaries due to more and larger cells. pro parthenocarpy is facultative because seeded fruits were obtained by manual pollination. Most pro pistils had exserted stigmas, thus preventing self-pollination, similar to wild-type pistils treated with GA3 or auxins. However, Style2.1, a gene responsible for long styles in noncultivated tomato, may not control the enhanced style elongation of pro pistils, because its expression was not higher in pro styles and did not increase upon GA3 application. Interestingly, a high percentage of pro flowers had meristic alterations, with one additional petal, sepal, stamen, and carpel at each of the four whorls, respectively, thus unveiling a role of SlDELLA in flower organ development. Microarray analysis showed significant changes in the transcriptome of preanthesis pro ovaries compared with the wild type, indicating that the molecular mechanism underlying the parthenocarpic capacity of pro is complex and that it is mainly associated with changes in the expression of genes involved in GA and auxin pathways. Interestingly, it was found that GA activity modulates the expression of cell division and expansion genes and an auxin signaling gene (tomato AUXIN RESPONSE FACTOR7) during fruit-set. PMID:22942390

  12. Fotometria CCD della cometa Hale-Bopp.

    NASA Astrophysics Data System (ADS)

    Mikuz, H.; Dintinjana, B.

    1999-10-01

    This contribution is based on the authors' five-year program of CCD photometry of faint comets, performed mainly with 20-cm and 36-cm telescopes, V filter and two CCDs. Recent apparition of comet Hale-Bopp proved to be an excellent opportunity to test the observing method, instrumentation and software even on a very bright comet. The authors found that their V observations of faint comets tend to be systematically fainter by 0.5 - 1 magnitude in spite of the fact that the CCDs record systematically more coma than human eye. The authors explained this with the fact that the human eye and V filter passbands are not the same and have thus different response to C2 which is the main emission from the coma. Additional troubles may appear with bright comets that have prominent inner tails, composed mainly of dust particles. In case of comet Hale-Bopp, it is shown that by carefully choosing the aperture radius, the resulting total V magnitudes are in good agreement with total values of experienced visual observers.

  13. [Ricerca storica sul contributo di Cristina Trivulzio di Belgiojoso all'assistenza infermieristica].

    PubMed

    Bertozzi, Simona; Di Mauro, Stefania; Manzoni, Edoardo

    2016-01-01

    The aim of this historical research project is to examine the contribution of Cristina Trivulzio Princess of Belgiojoso to the development of the nursing organisation in Italy. Specifically, the analysis focuses on the period of the Roman Republic, as in 1849 Cristina Trivulzio (Princess of Belgiojoso) was one of the person in charge of the service of caring and rescuing of the injured. For this reason, nineteen volumes were analysed out of the one hundred twenty-nine volumes found in five Italian historical archives. All documentary sources were evaluated accordingly to the Chabod historical research method (1999), while for their critical interpretation a scheme of analysis was created. As a result, five topics clearly emerged from the data collected: organisation, health politics, training, research and nursing. To sum up, this research reveals that the contributions of Cristina Trivulzio are so relevant that she may be counted among the founders of the italian nursing organisation. PMID:27191519

  14. Una ricerca sul bilinguismo precoce italo-inglese (Research on Early Italian-English Bilingualism).

    ERIC Educational Resources Information Center

    Taeschner, Traute; And Others

    1978-01-01

    The purpose of this study was to attempt to verify the theory of Taeschner and Volterra (1976) that bilingual children pass through three distinct phases while becoming perfectly bilingual. The 12 subjects were English-Italian bilingual children between the ages of 1.6 and 4.6. (CFM)

  15. [Valutare le esperienze positive e negative: la validazione di una nuova misura del benessere in una popolazione italiana].

    PubMed

    Corno, Giulia; Molinari, Guadalupe; Baños, Rosa Maria

    2016-01-01

    RIASSUNTO. Lo scopo del presente studio è quello di esplorare le proprietà psicometriche di uno strumento di misurazione dell'affetto, la Scala di Esperienze Positive e Negative (SPANE), all'interno di una popolazione italiana. Gli esiti dell'analisi fattoriale confermativa comprovano l'attesa struttura a due fattori, sentimenti positivi e negativi. Le correlazioni con altre dimensioni (per es., ansia, depressione, affetto, aspettative future) confermano i risultati ottenuti con le precedenti versioni della scala: affetto negativo, ansia, depressione e aspettative future negative correlano positivamente con la subscala di esperienze negative e negativamente con la subscala di esperienze positive dello SPANE. In conclusione, i risultati del nostro studio dimostrano che la versione italiana dello SPANE presenta caratteristiche psicometriche simili a quelle mostrate dalla versione orginale e da successive validazioni dello strumento in altre lingue. La scala presenta, inoltre, affidabilità e validità fattoriale. Lo SPANE è un indice utile dal punto di vista clinico che può fornire informazioni rilevanti circa l'esperienza emotiva e il benessere della persona. Nonostante ulteriori studi siano necessari per confermare le caratteristiche psicometriche della scala, la presente validazione della versione italiana dello SPANE può contribuire ad ampliare la ricerca nell'ambito del benessere in una popolazione Italiana. PMID:27362822

  16. Callisto III e la Cometa di Halley: la ricerca di Johan Stein SJ tra leggenda e storia

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    The Dutch Jesuit astronomer Johan Stein published in 1909 a text about the legend on a papal bull of Pope Callixtus III against the comet of 1456. This comet happened to be the Halley's one. The original documents, either chronicles and observations, and coeval testimonies are deeply investigated by Stein and the falsity of that legend is clearly demonstrated. In the occasion of the 200 years of the restoration of the Societas Jesu made in 1814 by Pope Pius VII an Italian edition of the full text of Johan Stein is here presented.

  17. High blood pressure and syncope: orthostatic hypotension as a link.

    PubMed

    Rafanelli, Martina; Ungar, Andrea

    2016-01-01

    The prevalence of hypertension increases with the age. Diagnostic criteria are the same as for the young, but in older adults isolated systolic hypertension is more frequent, due to loss of vascular compliance. Blood pressure should be measured on both sides in the seated position, moreover in the supine and upright position to detect orthostatic hypotension. Ambulatory blood pressure monitoring is useful to detect white coat hypertension and masked hypertension, to tailor the treatment and search for diurnal and nocturnal blood pressure pattern abnormalities. Given that frailty can affect the relationship between blood pressure and mortality, the clinician should properly evaluate and monitor physical performance and cognitive status, throughout specific tools, as the Fried Frailty Phenotype, aiming at a systolic blood pressure target between 130 and 150 mmHg. Before starting hypotensive drugs, a careful risk and benefits' evaluation should be performed given the high risk of hypertension and hypotension consequences and the frequent coexistence of orthostatic hypotension, which predisposes to syncope and falls.  RiassuntoLa prevalenza dell'ipertensione arteriosa aumenta con l'età. I criteri diagnostici sono gli stessi previsti nel giovane, nell'anziano tuttavia si osserva più frequentemente ipertensione sistolica isolata, dovuta a perdita dell'elasticità vascolare. Ai fini diagnostici, la misurazione della pressione arteriosa bilateralmente in posizione seduta, deve essere integrata con la ricerca di ipotensione ortostatica. Il monitoraggio della pressione arteriosa nelle 24h è indicato per la conferma diagnostica, per la ricerca di "white coat hypertension" e "masked hypertension", per monitorare la risposta terapeutica e ricercare alterazioni del ritmo circadiano. I benefici della terapia antiipertensiva sono noti, tuttavia un trattamento troppo aggressivo si è dimostrato dannoso, rendendo la gestione dell'ipertensione arteriosa nell'anziano ancora pi

  18. Evoluzione fotometrica della cometa C/2006 M4 (Swan)

    NASA Astrophysics Data System (ADS)

    Milani, G.; Guido, E.; Ligustri, R.; Montanucci, F., Nicolini, M.; Sostero, G.; Trabati, R.

    2010-04-01

    The apparition of comet C/2006 M4 (SWAN) displayed a complex evolution characterized by a 2 magnitude outburst around the 24th of October 2006, related to a nucleus fragmentation event that produced a cloud of debris observed on the 7th of Novembre. The general evolution shows a complex trend indicating a probable further erosion process and/or fragmentation phaenomena.

  19. Il fenomeno della "goccia nera" e l'astigmatismo

    NASA Astrophysics Data System (ADS)

    Horn D'Arturo, Guido

    2004-03-01

    The Venus transit on the Solar disc was for the first time observed in 1639, using "modern" instruments, by Gassendi. Yet since the following transit, an effect was perceived which modified Venus and Sun profiles at the moments of the first and second visual contact between the two celestial bodies. This effect was called "gutta nigra", i.e. black drop. Horn d'Arturo was widely interested in studies on the vision and on its effects on astronomical observations. In the following paper, he suggested what probably was the first correct explanation of the black drop effect. As stated on the Dictionary of Scientific Biography (ad vocem), Horn "clarified the effect on vision, especially in the astigmatic eye, of the suture of the eye lens and the formation of the so-called black drop".

  20. Opera in Kindergarten: "La Principessa della sacco di carta"

    ERIC Educational Resources Information Center

    Honeck, Ellen; Jones, Shannon

    2009-01-01

    When designing curriculum, the unique characteristics of young, gifted students must be carefully considered. Many have a highly defined sense of justice or fairness, a precocious ability to manipulate language or number systems, a need to see the connections between learning experiences, a heightened curiosity, and an ability to conceive a…

  1. Misura dell'azimut della Piramide Cestia col Sole

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2016-05-01

    The Pyramid of Caius Cestius in Rome has been built in 330 days in 12 b.C. The instants when the Sun rays are grazing to the East face of the Pyramid have been observed in different days, writing a table of solar azimut and altitudes. Extrapolating for zero altitude, the azimut 201.8°±1.4° of the Pyramid is obtained; it corresponds to the azimut 204.27° of the Sun when the shadow of the lightning rod divides in two equal parts the North face.

  2. Algoritmi per il calcolo dell'epatta della Luna

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2016-05-01

    On the sides of the chair of St. Hyppolitus in the Vatican Libray there is a series of 112 epacts, used for calculating the date of Christian Easter valid for 112 years from 222 AD. The algorithm of octaëteris or 8 civil (julian) years=99 lunar months and a correction of three days each 16 years or one day each 5, 5, 6 years are discussed. Four complete solar cycles (28 years), after which the sequence of the day of the week are repeating, are included in 112 years as well as 7 groups of 16 years; 112 is the minimum common multiple between the double octaëteris (16 years) and the solar cycle (28).

  3. The fabbrica della penicillina in postwar Italy: an institutionalist approach.

    PubMed

    Taroni, Francesco

    2014-01-01

    This paper focuses on the motives and long-term effects of the momentous decision to build a world-class biomedical research laboratory, the International Center for Chemical Microbiology, at the Istituto Superiore di Sanità in Rome, rather than develop domestic production of penicillin to meet the needs of a destitute postwar Italy. An institutionalist approach will provide a richer vision of the intersections of scientific and national political history in postwar Italy and the Cold War. The Center failed in its modernising mission due to an insular mentality producing an 'enclosure effect' against the State, the healthcare system and the pharmaceutical industry. The absence of a scientific base together with an economic policy of 'liberal protectionism' that placed premiums on import tariffs and the licensing of foreign products explains the path dependency of the pharmaceutical industry during the postwar years and its demise in the 1960s. PMID:26054217

  4. Il volto della Luna da Plutarco a Newton. Storia di una teoria ellenistica della gravità

    NASA Astrophysics Data System (ADS)

    Cappi, Alberto

    2013-03-01

    In his dialogue "De Facie quae in Orbe Lunae apparet", Plutarch includes a discussion on a non-Aristotelian theory of gravity. I describe how this theory could be developed during the Hellenistic period and its influence on the cosmological debate along the centuries until the beginning of modern science. (Text in Italian)

  5. PREFACE: Young Researcher Meeting in Rome 2012

    NASA Astrophysics Data System (ADS)

    Agostini, Fabio; Cattani, Giordano; Mazzaferro, Luca; Migliaccio, Marina; Pietrobon, Davide; Ricci Pacifici, Daniel; Stellato, Francesco; Veneziani, Marcella

    2012-10-01

    Conference logo At its third edition, the Young Researcher Meeting in Rome (YRMR) proves to be a growing event in the Italian scientific panorama. The high-quality content of the abstracts submitted to the scientific committee resulted in an exciting conference, held, for the second time, at the University of Rome 'Tor Vergata' on 20 January 2012. A busy schedule covered a large variety of cutting-edge science topics: fundamental interactions, particle physics, cosmology, astrophysics, condensed matter and biomedical physics. The broad range of the subjects discussed is the distinctive feature of the YRMR, a meeting aimed at enhancing the synergy among complementary branches of science by stimulating a fruitful exchange between theoretical, experimental and computational physics. Promoting collaborations between PhD students, postdoctoral fellows and young researchers creates a solid scientific network with an open-minded approach to discovery. In this volume, we collect the contributions that have been presented both in the form of talks and of posters. YRMR Organising and Editorial Committee Fabio Agostini (fabio.agostini@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma Italy Giordano Cattani (giordano.cattani@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' INFN sezione di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma Italy Luca Mazzaferro (luca.mazzaferro@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' INFN sezione di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma Italy Marina Migliaccio (migliaccio@ifca.unican.es) Instituto de Fisica de Cantabria, Edificio Juan Jorda, Avenida de los Castros, E-39005 Santander, Cantabria Spain Davide Pietrobon (davide.pietrobon@jpl.nasa.gov) Jet Propulsion Laboratory - California Institute of Technology 4800 Oak Grove Drive 169-237 91109 Pasadena, CA USA Daniel Ricci Pacifici

  6. Lo Gnomone Clementino Astronomia Meridiana in Basilica

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2014-05-01

    Costruito per chiara volontà del papa 70 anni dopo il caso Galileo, lo Gnomone Clementino è un grande telescopio solare che non fa uso di lenti a 92 anni dall’invenzione del cannocchiale. Queste due caratteristiche basterebbero da sole a giustificare l’interesse verso questo strumento. L’astronomia meridiana è alla base dell’astrometria e dell’astrofisica moderna. Lo Gnomone Clementino sta oggi all’astronomia, come il veliero “Amerigo Vespucci” sta alla Marina Italiana. E’ possibile svolgere ogni genere di osservazione e studio su questo strumento, e dal 2002 vi tengo lezioni teorico-pratiche del corso di Storia dell’Astronomia e La Terra nel Sistema Solare della Sapienza, Università di Roma, Facoltà di Lettere e Filosofia. Questo testo aggiunge alcuni tasselli alla ricerca storica sulla meridiana, appoggiandosi, com’è naturale, sulle spalle di giganti che mi hanno preceduto in questi studi. In particolare la misura dell’azimut della meridiana, ed il suo inquadramento tra gli strumenti simili ed alcuni studi di astrometria sui dati del 1701-1703 di Bianchini, che sono apparsi fin’ora soltanto su riviste specializzate ed in Inglese vengono qui proposti in Italiano e semplificati, per valorizzare sempre più questa straordinaria opera d’arte e di scienza.

  7. Three models intercomparison for Quantitative Precipitation Forecast over Calabria

    NASA Astrophysics Data System (ADS)

    Federico, S.; Avolio, E.; Bellecci, C.; Colacino, M.; Lavagnini, A.; Accadia, C.; Mariani, S.; Casaioli, M.

    2004-11-01

    In the framework of the National Project “Sviluppo di distretti industriali per le Osservazioni della Terra” (Development of Industrial Districts for Earth Observations) funded by MIUR (Ministero dell'Università e della Ricerca Scientifica --Italian Ministry of the University and Scientific Research) two operational mesoscale models were set-up for Calabria, the southernmost tip of the Italian peninsula. Models are RAMS (Regional Atmospheric Modeling System) and MM5 (Mesoscale Modeling 5) that are run every day at Crati scrl to produce weather forecast over Calabria (http://www.crati.it). This paper reports model intercomparison for Quantitative Precipitation Forecast evaluated for a 20 month period from 1th October 2000 to 31th May 2002. In addition to RAMS and MM5 outputs, QBOLAM rainfall fields are available for the period selected and included in the comparison. This model runs operationally at “Agenzia per la Protezione dell'Ambiente e per i Servizi Tecnici”. Forecasts are verified comparing models outputs with raingauge data recorded by the regional meteorological network, which has 75 raingauges. Large-scale forcing is the same for all models considered and differences are due to physical/numerical parameterizations and horizontal resolutions. QPFs show differences between models. Largest differences are for BIA compared to the other considered scores. Performances decrease with increasing forecast time for RAMS and MM5, whilst QBOLAM scores better for second day forecast.

  8. Elaborare in sardo, elaborare in italiano. Una ricerca nella scuola media (Uses of Sardinian and Italian: Research in the Junior High School).

    ERIC Educational Resources Information Center

    Pinto, Maria Antonietta; Migaleddu, Maria Vittoria

    1989-01-01

    Presents a historic overview and analysis of the Sardinian language (actually a system of dialects), and defines diglossia and bilingualism. A discussion focuses on research conducted in two junior high schools to determine the differences in linguistic behavior in two contrasting situations (village and city) in regard to Sardinian and Italian.…

  9. Il problema della scelta e della continuita della lingua straniera nella scuola dell'obbligo (Problems of Choice and Continuity of a Foreign Language in Elementary and Junior High School).

    ERIC Educational Resources Information Center

    Gotti, Maurizio

    1987-01-01

    Discusses two current problems with foreign language study in Italy: (1) Students are frequently unable to study English, the most popular language, because of a lack of qualified English teachers; and (2) Students sometimes cannot continue studying in junior high school the language they began in elementary school. (CFM)

  10. DUSTER: collection of meteoric CaO and carbon smoke particles in the upper stratosphere .

    NASA Astrophysics Data System (ADS)

    Della Corte, V.; Rietmeijer, F. J. M.; Rotundi, A.; Ferrari, M.; Palumbo, P.

    Nanometer- to micrometer-size particles present in the upper stratosphere are a mixture of terrestrial and extra-terrestrial origins. They can be extraterrestrial particles condensed after meteor ablation. Meteoric dust in bolides is occasionally deposited into the lower stratosphere around 20 km altitude. Nanometer CaO and pure carbon smoke particles were collected at 38 km altitude in the upper stratosphere in the Arctic during June 2008 using DUSTER (Dust in the Upper Stratosphere Tracking Experiment and Retrieval), a balloon-borne instrument for the non-destructive collection of solid particles between 200 nm to 40 microns. We report the collection of micron sized CaCO_3 (calcite) grains. Their morphologies show evidence of melting and condensation after vaporization suggest at temperatures of approximately 3500 K. The formation environment of the collected grains was probably a dense dust cloud formed by the disintegration of a carbonaceous meteoroid during deceleration in the Earth� atmosphere. For the first time, DUSTER collected meteor ablation products that were presumably associated with the disintegration of a bolide crossing the Earth's atmosphere. The collected mostly CaO and pure carbon nanoparticles from the debris cloud of a fireball, included: 1) intact fragments; 2) quenched melted grains; and 3) vapor phase condensation products. The DUSTER project was funded by the Italian Space Agency (ASI), PRIN2008/MIUR (Ministero dell'Istruzione dell'Universitá e della Ricerca), PNRA 2013(Piano Nazionale Ricerca Antartide). CNES graciously provided this flight opportunity. We thank E. Zona and S. Inarta at the Laboratorio di Fisica Cosmica INAF, Osservatorio Astronomico di Capodimonte-Universitá di Napoli Parthenope. F.J.M.R. was supported by grant NNX07AI39G from the NASA Cosmochemistry Program. We thank three anonymous reviewers who assisted us in introducing our new instrument.

  11. The EEE Project

    NASA Astrophysics Data System (ADS)

    Abbrescia, M.; An, S.; Antolini, R.; Badala, A.; Baldini Ferroli, R.; Bencivenni, G.; Blanco, F.; Bressan, E.; Chiavassa, A.; Chiri, C.; Cifarelli, L.; Cindolo, F.; Coccia, E.; de Pasquale, S.; di Giovanni, A.; d'Incecco, M.; Fabbri, F.L.; Frolov, V.; Garbini, M.; Gustavino, C.; Hatzifotiadou, D.; Imponente, G.; Kim, J.; La Rocca, P.; Librizzi, F.; Maggiora, A.; Menghetti, H.; Miozzi, S.; Moro, R.; Panareo, M.; Pappalardo, G.S.; Piragino, G.; Riggi, F.; Romano, F.; Sartorelli, G.; Sbarra, C.; Selvi, M.; Serci, S.; WIlliams, C.; Zichichi, A.; Zuyenski, R.

    The EEE (/Extreme Energy Event/) Project is an experiment for the study of very high-energy extensive air showers, actually starting in Italy. It is based on the detection of the shower muon component by means of a network of tracking detectors, installed in Italian High Schools. The Project, supported by the Ministero dell’Università e della Ricerca (MIUR), Istituto Nazionale di Fisica Nucleare (INFN), European Organization for Nuclear Research (CERN) and Museo Storico della Fisica e Centro Studi e Ricerche “E. Fermi” has been conceived by its leader Professor Antonino Zichichi. In its first phase the detector telescopes will be installed in 21 High Schools in 7 piloting cities all over Italy. The network will soon be heavily upgraded by increasing the number of High Schools and cities. The single tracking telescope is composed by 3 large (~ 2 m2 ) Multi-gap Resistive Plate Chambers (MRPC), realized with float glass electrodes. The use of particle detectors based on such MRPCs will allow to determine with a very high accuracy the direction of the axis of cosmic ray showers initiated by primaries of ultra-high energy, together with a high temporal resolution. The first MRPC telescope, installed in the Liceo Scientifico “B.Touschek” in Grottaferrata near the LNF-INFN site (nearby Rome), is successfully running. By the end of year 2007, the installation of the other telescopes will open the way for the first search of high-energy cosmic rays distant coincidences. In the future, serving many High Schools scattered all over the Italian territory, the EEE Project will also allow to investigate coincidences between multiple primaries producing distant showers. Here we present the experimental apparatus and its tasks.

  12. Primi risultati della campagna osservativa sulla cometa C/2001 Q4 (NEAT)

    NASA Astrophysics Data System (ADS)

    Milani, Giannantonio; Ligustri, Rolando; Tirelli, Diego

    2005-04-01

    The first results of the C/2001 Q4 observation campaign are presented. The comet was observed with visual, photographic and CCD techniques. Both photometry and imaging show an active comet even if fainter than expected, especially after the perihelion passage. The coma presented halos and spiral-like features recalling those displayed by other comets like Hale-Bopp and Ikeya-Zhang. Visual and CCD light curves are analyzed and first tests with narrowband filters were performed with the goal of obtaining more accurate Afrho measurements. Narrowband filters (647-650 nm) proved useful also for dust tail analysis and are now adopted as standard for the CARA project.

  13. L'apparizione della cometa C/2002 O4 (Hönig)

    NASA Astrophysics Data System (ADS)

    Milani, Giannantonio

    2003-07-01

    The comet C/2002 O4 (Hönig) underwent a rapid evolution since its discovery on 22 July 2002 through its sudden disappearance occurred about its passage at the perihelion (1 October). The comet initially displayed a tail revealing a gaseous component which, at its turn, rapidly disappeared at the end of September, probably because of the comet's nucleus disruption.

  14. Osservazioni radio di Giove durante gli impatti della cometa Shoemaker-Levy 9.

    NASA Astrophysics Data System (ADS)

    Bressan, G.

    1994-10-01

    Jupiter's decametric radio emissions lowered during the week interested by the comet impacts. Each event was preceded, as loss frequently followed, by outbursts which increased the radio emission between three and four times the ground noise.

  15. Studio morfologico della cometa P/Swift-Tuttle durante l'apparizione del 1992.

    NASA Astrophysics Data System (ADS)

    Favero, G.; Favero, G.; Milani, G. A.

    1996-02-01

    The study of hundreds visual, photographic and CCD observations of comet 109P/Swift-Tuttle made by amateurs and mostly published in popular magazines allowed the authors to estimate the expansion velocities of the coma and the behaviour of the plasma tail. The photometric and morphologic behaviour is accounted for by a monolithic nucleus, rotating in 2.92 d and erupting gas and dust from three restricted areas of its surface.

  16. Loss-of-function of DELLA protein SLN1 activates GA signaling in barley aleurone

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Gibberellic acid (GA) is a key phytohormone regulating seed germination and seedling growth. In cereal species, GA is synthesized in embyros and then translocated to aleurone tissues where it induces production of hydrolytic enzymes for mobolization of seed storage reserve. De-embyronated barley a...

  17. Ipotesi astronomiche sul foro della colonna augustea di Santa Maria in Aracoeli

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2014-05-01

    The third column of the left row in the Basilica of Santa Maria in Aracoeli in Rome, has a hole carved in it, of probable astronomical use. The tube aims at a point a 116 degrees of azimut, East-South-East and 17.3 degrees of altitude, the Sun could be aimed in that direction at 07:00 UTC of 12 october and 7:25 UT of march 2, but the view is obstructed by the church's building. Same situation for the 3.9 magnitude alpha Monocerotis, visible 2000 years ago, taking into account the precession. Other hypotheses on the meridian astronomical use of this hole are here reviewed. In the case of solar observations it cannot be for meridian transits in Rome, since the lower transits occurs now at 24.5 degrees at winter solstice; we investigate the case of stellar transits, of declination 30.8 degrees South. Many alignments are possible leaving the azimut as free parameter, being the columun not in its original collocation, but here we investigate only the meridian transits, and no bright stars could have been seen through that hole, taking also into account the precession 2000 years ago. We conclude that this hole was probably not used for meridian observations.

  18. Osservare le eclisse solari dal bordo della fascia di totalità

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino; Oliva, Pietro

    2006-06-01

    Observing Solar eclipses at the totality path limits allows to measure solar diameter variations and lunar ephemerides corrections with an accuracy of a few hundredths of arcsecond. After a review of secular variations of solar diameter, presenting the eclipses of Rome 1567, London 1715, and New York City 1925, we present the current research on this topic and the phenomena occurring on the edges of the totality path. For the forthcoming eclipses, the location of observers must be chosen after calculations of limits and simulations of bead appearances and timings. We present our example in Valoria La Buena (Spain) for the 3 October, 2005 eclipse. Small unfiltered refracting telescopes, equipped with projection screen are recommended for standard observations, in order to avoid artificial reduction in the observed solar diameter due to filtering effects. Preliminary data reductions on eclipses of 1995 India and 1998 Caribbean, and on 2004 Venus Transit are also presented.

  19. Il Calcolo della Pasqua: Vittorio d'Aquitania Dionigi il Piccolo e Abbone di Fleury

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2014-05-01

    The Easter calculus is a story of ephemerides approximations, with appropriate algorithms, as well as the reformations of the calendar dealed with tropical year's approximations. The calculus made by Victorius of Aquitania, Dyonisius Exiguus and Abbo of Fleury, based on 532 years Easter period in Julian calendar are discussed, including the corrections ad hoc of the algorithms, like the saltus lunae.

  20. Il dolore in ambito urologico: aspetti fisiopatologici del dolore e della cronicità

    PubMed

    Bonezzi, Cesare; Demartini, Laura; Angelini, Lucia; Armiento, Luciana; Jallous, Hussein

    2014-12-01

    Pain in urology: pathophysiological aspects of pain and chronicityChronic pain has been traditionally defined by pain duration, but this approach has limited empirical support and does not account for chronic pain multidimensionality. Defining chronic pain solely by duration is based on the view that acute pain signals potential tissue damage, whereas chronic pain results from central sensitization in which pain is sustained after nociceptive inputs have diminished. Chronic urological pain is a prevalent condition, which can represent a major challenge to health care providers due to its complex aetiology and poor response to therapy. In most cases, clear signs of on-going tissue trauma, inflammation or infection are not present. Despite this, more underhanded pathophysiological mechanisms, affecting the urinary system or other pelvic organ systems (musculoskeletal, neurologic, urologic, gynaecologic) and some psychological aspects may be present. In this article, some pathophysiological aspects of visceral pain are discussed; the definition of 'chronic pain', the mechanism of action of drugs used in the treatment of pain and the rationale for association therapy are also reviewed. PMID:25451874

  1. Medicean telescopes. The collection of the Istituto e Museo di Storia della Scienza, Florence

    NASA Astrophysics Data System (ADS)

    Miniati, Mara

    The Institute and Museum of the History of Science in Florence preserves an important collection of 17th telescopes coming from the Medicean heritage. The paper will introduce these instruments and their connection with the contemporary astronomical and optical debate.

  2. I termini di Cassini e Laplace della rifrazione atmosferica misurati alla Meridiana Clementina

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2016-05-01

    The measures of the atmospheric refraction terms of Cassini and Laplace are made at the Clementine Meridian Line of S. Maria degli Angeli in Rome by comparing the observed positions of the center of the Sun with the ones calculated without atmosphere. Three linear systems with the data of 21 Dec 2015; 18 Jan and 6 Feb 2016 give the refraction terms of tan(z) and tan3(z).

  3. Gibberellin hormone signal perception: down-regulating DELLA repressors of plant growth and development

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The gibberellin (GA) hormone signal is perceived by a receptor with homology to hormone sensitive lipases, GID1 (GA-INSENSITIVE DWARF1). This leads to GA-stimulated responses including stem elongation, seed germination, and the transition to flowering. GA-binding enables GID1 to interact with and ...

  4. La Geometria astronomica di Gerberto e le metodologie della prima misura geodetica di Eratostene

    NASA Astrophysics Data System (ADS)

    Chirri, Maurizio

    The first geodetic measurements in the Hellenistic period started the scientific geography. Cartographic representations were finally placed on a quantitative basis, the subsequent geographical and naturalistic favored a rapid development of all related sciences. The embryos of modern Earth Sciences, can be traced in the context of such a concentration of knowledge and investigation. When appeared the Geometry of Gerbert, some lines of transmission of scientific knowledge on the available classical works, now lost, may have been active and are here investigated, to shed light on methodological aspects of these old measurements concerning also the use of some basic astronomical instruments for measuring angles and distances.

  5. Singing in "La Voce Della Luna" Italian Women's Choir in Melbourne, Australia

    ERIC Educational Resources Information Center

    Southcott, Jane; Joseph, Dawn

    2015-01-01

    Australia is a country of ongoing migration that embraces diversity, creative expression and cultural activity. Membership of community music groups by older people can enhance life quality, and may provide a space through which cultural and linguistic identity may be shared and celebrated. This qualitative phenomenological case study explores…

  6. 78 FR 5555 - Culturally Significant Object Imported for Exhibition Determinations: “Piero della Francesca in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-25

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE...'' SUMMARY: Notice is hereby given of the following determinations: Pursuant to the authority vested in me by the Act of October 19, 1965 (79 Stat. 985; 22 U.S.C. 2459), Executive Order 12047 of March 27,...

  7. [The activities of Santa Maria Della Scala through 1318 status and its frescoes].

    PubMed

    Pinault, Claire

    2002-01-01

    Siena's Hospital was created in the 10th or 11th century, and still owns a rich documentation of his history, stored at the city's state Archives the general organization of the Hospital in the 14th century is accurately described in the 1318 statue. Moreover, during the 15th century frescoes representing its activities were commissioned by the Hospital. They decorated the Pellegrinaio, the main room and the infirmary. The comparison of these two sources from different periods enables us to ask ourselves why the hospital felt compelled to issue what could appear as redundant illustration of the hospital activities. PMID:12749334

  8. Per una pedagogia della comprensione interculturale (A Method for Teaching Intercultural Understanding).

    ERIC Educational Resources Information Center

    Titone, Renzo

    1986-01-01

    Defines true bilingualism as biculturalism, explains what "understanding" another culture means, and describes how to evaluate the effectiveness of teaching methods for students from different cultures. Ideally, individuals learning a second language will acquire a "metacultural" consciousness that permits them to be comfortable in any cultural…

  9. PROCERA encodes a DELLA protein that mediates control of dissected leaf form in tomato

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Leaves of seed plants can be described as simple, where the leaf blade is entire, or dissected, where the blade is divided into distinct leaflets. Mechanisms that define leaflet number and position are poorly understood and their elucidation presents an attractive opportunity to understand mechanism...

  10. The MAGIC-5 CAD for nodule detection in low dose and thin slice lung CTs

    NASA Astrophysics Data System (ADS)

    Cerello, Piergiorgio; MAGIC-5 Collaboration

    2010-11-01

    Lung cancer is the leading cause of cancer-related mortality in developed countries. Only 10-15% of all men and women diagnosed with lung cancer live 5 years after the diagnosis. However, the 5-year survival rate for patients diagnosed in the early asymptomatic stage of the disease can reach 70%. Early-stage lung cancers can be diagnosed by detecting non-calcified small pulmonary nodules with computed tomography (CT). Computer-aided detection (CAD) could support radiologists in the analysis of the large amount of noisy images generated in screening programs, where low-dose and thin-slice settings are used. The MAGIC-5 project, funded by the Istituto Nazionale di Fisica Nucleare (INFN, Italy) and Ministero dell'Università e della Ricerca (MUR, Italy), developed a multi-method approach based on three CAD algorithms to be used in parallel with a merging of their results: the Channeler Ant Model (CAM), based on Virtual Ant Colonies, the Dot-Enhancement/Pleura Surface Normals/VBNA (DE-PSN-VBNA), and the Region Growing Volume Plateau (RGVP). Preliminary results show quite good performances, to be improved with the refining of the single algorithm and the added value of the results merging.

  11. Una Ricerca sull'Applicazione del TALB (Test di Attitudine Linguistica per Bambini) nella Scola Elementare [Research on the Application of the TALB (A Test for Linguistic Aptitude for Children) in Elementary School].

    ERIC Educational Resources Information Center

    Acone, Ilario

    1992-01-01

    Discusses the administration of the Test di Attitudine Linguistica per Bambini (TALB) to students 7-9 years of age. Correlations between the TALB and achievement tests administered at the end of the course were very high. It is concluded that aptitude is a main factor in successful learning of a second language. (16 references) (LET)

  12. 78 FR 52601 - Culturally Significant Object Imported for Exhibition; Determinations: “Visiting Masterpiece...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-23

    ... Francesca's Senigallia Madonna, an Italian Treasure, Stolen and Recovered'' and ``Piero della Francesca...: Piero della Francesca's Senigallia Madonna, An Italian Treasure, Stolen and Recovered'' at the Museum...

  13. L'evento di disconnessione del 24 marzo 1996 nella coda di plasma della cometa 1996 B2 Hyakutake.

    NASA Astrophysics Data System (ADS)

    Guidolin, E.; Milani, G.

    1998-12-01

    The close approach of comet 1996 B2 (Hyakutake) was observed in very good sky conditions during the night between March 24 and 25. A sequence of photographs taken with a 180 mm f/2,8 telephoto lens shows a knot with a high proper motion in the plasma tail related to a disconnection event. The authors find that the average velocity of the plasma knot was 32.3 km/sec on March 25.0, 53.6 km/sec on March 25.7 and 76.1 km/sec on March 26.5. The average acceleration due to the solar wind was close to 0.0003 km/sec. Extrapolating the data one also finds that the beginning of the disconnection event was around March 24.7.

  14. La cometa 9P Tempel 1 nell'apparizione 2005. Monitoraggio durante la fase di impatto della missione Deep Impact

    NASA Astrophysics Data System (ADS)

    Milani, Giannantonio; Sostero, Giovanni; Trabatti, Roberto; Vinante, Carlo

    2006-06-01

    Comet 9P/Tempel 1 was constantly monitored during the 2005 apparition by observers participating in the CARA project (http://cara.uai.it). R and I (Cousins), as well as 647-650 nm narrowband filter photometric data were used to produce Afrho quantity measuremnts. Afrho value peaked about 300 cm approximately 83 days before perihelion, with a general behaviour that closely matches the one of previous apparitions. Around the time of the impact of the Deep Impact module a short temporary increase was detected, but Afrho values and the coma profile returned to a normal state within approximately 3 days. The dust cloud produced by the impact was clearly seen in the images obtained on July 4 and 5 as well as a temporary disturbance in the average photometric profile in the coma. No permanent long-time effects were detected in the average Afrho behaviour, which matches the trend observed in previous apparitions fairly well.

  15. Osservazione fotografica della coda di plasma dele comete. Campagna osservativa per la cometa P/Brorsen Metcalf.

    NASA Astrophysics Data System (ADS)

    Cimatti, A.; Milani, G. A.

    1989-04-01

    The authors examined the importance of the study of plasma tails of the comets. They supplied all usefull suggestions to study them photographically. Particular attention is suggested to the comet P/Brorsen-Metcalf.

  16. [I costi farmacologici della terapia di conversione con farmaci biologici nel carcinoma del colon-retto con metastasi epatiche].

    PubMed

    Giuliani, Jacopo; Bonetti, Andrea

    2016-08-01

    Riassunto. Lo scopo di questo studio è quello di valutare i costi dei farmaci (con particolare riferimento alle terapie con farmaci biologici) utilizzati nella terapia di conversione in una popolazione non selezionata di pazienti affetti da carcinoma del colon-retto in stadio avanzato, al fine di ottenere una resezione epatica R0. In questa rassegna sono stati selezionati i report completi e gli aggiornamenti di tutti gli studi clinici randomizzati (di fase II e fase III) che confrontassero almeno 2 regimi di terapia con farmaci biologici in prima linea in pazienti affetti da carcinoma del colon-retto in stadio avanzato di malattia. I costi dei farmaci sono stati ricavati dalla nostra Farmacia Ospedaliera e sono espressi in euro (€). Il nostro studio inizia con la valutazione di 683 abstract. 48 tria sono stati considerati adeguati per una successiva analisi. Una valutazione più approfondita ha portato all'esclusione di 37 trial, lasciando alla valutazione finale 11 studi clinici randomizzati (3 trial di fase II, per un totale di 522 pazienti, e 8 studi di fase III, per un totale di 7191 pazienti). I costi dei farmaci utilizzati nella terapia di conversione aumentano con la sostituzione del 5-fluorouracile con la capecitabina e, in misura maggiore, con l'introduzione degli agenti biologici. In questo lavoro sono presentati due punti chiave. Primo, i costi degli agenti farmacologici utilizzati nei regimi di prima linea a base di agenti biologici più comunemente utilizzati nel trattamento del carcinoma del colon-retto in stadio avanzato sono molto variabili. Secondo, i dati di efficacia dei regimi pubblicati, in termini di tassi di resezione, dipendono dalla selezione dei pazienti, dalle caratteristiche del tumore e dal tipo di schema di terapia. PMID:27571559

  17. Prime linee-guida per l'uso della webcam bidirezionale con bambini affetti da patologia oncologica in fase avanzata.

    PubMed

    Giacon, B; Biassoni, V; Clerici, C A; Spreafico, F; Meazza, C; Veneroni, L; Massimino, M

    2014-07-14

    AIM:The paper presents guidelines for school staff on the use of two-way webcam for the sake of school-continuity with school children suffering from malignant tumors. The proposed guidelines are designed to prepare school staff about the interests of those involved in the interaction (sick child,classmates, teachers). PMID:25018083

  18. L'Astronomia del Venerdí Santo, l'eclissi di Luna e l'ora della Sindone

    NASA Astrophysics Data System (ADS)

    Sigismondi, Costantino

    2014-05-01

    Dating the crucifixion on friday 3 April 33, when, at sunset a partially eclipsed Moon rised allows to interprete the speech of Saint Peter (Acts 2:20=Joel 3:4) on the day of Pentecost as including an account of this eclipse, as fulfillment of the Scriptures. Few minutes later the apparition of a third star of medium magnitude stated the beginning of the sabbatical rest: before that time Jesus has been buried, wrapped into the shroud. The observations of the equinoctial moonrise from the Mount of Olives on September 2013 are strongly in favour of the hypotehsis of possible observation of the lunar eclipse of 33 AD by Jewish people, as well as the sunrises observed from the Temple Institute terrace in Southern-East Jerusalem demonstrated that this eclipse could have been seen from Jerusalem. The depression of the horizon and the refraction allow to see Sun and Moon more than 1 degree below the geometrical horizon in the azimuthal direction ranging from 90 to 116, prologing to 16 minutes the duration of the partial lunar eclipse observed in 33AD. Texts of Matthew, Mark and Cyrillus of Jerusalem are also commented.

  19. PREFACE: Young Researcher Meeting, Trieste 2013

    NASA Astrophysics Data System (ADS)

    Agostini, F.; Antolini, C.; Aversa, R.; Cattani, G.; Martinelli, M.; Mazzaferro, L.; Migliaccio, M.; Paci, F.; Pietrobon, D.; Ricci Pacifici, D.; Stellato, F.; Veneziani, M.

    2013-12-01

    YRM logo The Young Researcher Meeting (www.yrmr.it) has been a rapidly growing event for the last few years. Together with other initiatives which have emerged in several research areas, the young researcher meeting marks the awareness and the desire of PhD students, postdoctoral fellows and young researchers to play a major role in scientific progress. Devoted to the discussion and the interchange of new developments and ideas in physics, the meeting is primarily aimed at graduate students and postdoctoral researchers, who are encouraged to present their work in an informal atmosphere. One of the main purposes of the conference is to create an international network of young researchers, both experimentalists and theorists, and fruitful collaborations across the different branches of physics. Born in Rome in 2009, after three editions that strengthened it, the Young Researcher Meeting 2013 was held in Trieste. Propelled by the past success, the fourth meeting was a two-day conference on 3-4June. It was sponsored by the International School for Advanced Studies - SISSA - and the University of Padova, thus acquiring an even further international drive. In this volume, we collect some of the contributions that were presented at the conference. They cover topics in astrophysics and cosmology, particle and theoretical physics, soft and condensed matter, biophysics and medical physics. YRM Organising and Editorial Committee Fabio Agostini (fabio.agostini@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma, Italy Claudia Antolini (claudia.antolini@sissa.it) SISSA - Scuola Internazionale Superiore di Studi Avanzati Via Bonomea 265, 34136 Trieste, Italy Rossella Aversa (raversa@sissa.it) SISSA - Scuola Internazionale Superiore di Studi Avanzati Via Bonomea 265, 34136 Trieste, Italy Giordano Cattani (giordano.cattani@gmail.com) Matteo Martinelli (mmartin@sissa.it) SISSA - Scuola Internazionale Superiore di

  20. [Psicopatologia, fattori psicosociali e obesità].

    PubMed

    Pinna, Federica; Sardu, Claudia; Orrù, Walter; Velluzzi, Fernanda; Loviselli, Andrea; Contu, Paolo; Carpiniello, Bernardo

    2016-01-01

    RIASSUNTO. Scopi. Gli obiettivi di questo studio sono stati la valutazione dell'associazione tra obesità, variabili socio-demografiche e psicopatologiche in un campione clinico di pazienti afferenti a un centro per la diagnosi e il trattamento dell'obesità, confrontati con un campione omogeneo di controllo costituito da soggetti di peso normale. Metodi. Nel contesto di un progetto di ricerca riguardante obesità e psicopatologia, è stata applicata una regressione logistica multivariata per esaminare l'associazione tra obesità e variabili demografiche e cliniche relative a un campione consecutivo di 293 pazienti obesi (48 maschi, 245 femmine, età media: 45,41±13,55, indice di massa corporea [BMI] 35,6±6,2), confrontato con un gruppo di controllo omogeneo appaiato di 293 soggetti non obesi (48 maschi, 245 femmine, età media 45,66±13,86, BMI medio 21,8±2,06). Tutti i soggetti sono stati valutati mediante un'intervista clinica strutturata, la Structured Clinical Interview per la diagnosi di asse I del DSM-IV (SCID-I) e per l'asse II DSM-IV (SCID-II). Risultati. L'analisi statistica multivariata ha dimostrato che lo status di casalinga e la presenza "lifetime" di disturbi di asse I e II e in generale della presenza di una qualsiasi diagnosi psichiatrica, e, in particolare, delle diagnosi di disturbo depressivo, d'ansia, del comportamento alimentare e di alcuni disturbi di personalità, sono associate in modo significativo a una maggiore probabilità di essere in sovrappeso oppure obesi. Le probabilità di essere sovrappeso/obesi si modificano in rapporto alle diverse combinazioni di fattori di rischio, aumentando da un valore di 32,3% per un individuo non esposto ad alcun fattore di rischio, a un valore di 86,7% per i soggetti esposti a tutti i fattori di rischio considerati. Conclusioni. La presenza di una diagnosi di asse I e/o II e lo stato di casalinga è indipendentemente associata a un maggiore rischio di sovrappeso/obesità. L'interazione di questi

  1. L'influenza dell'attenzione, della memoria e della discriminazione fonetica nell'apprendimento della seconda lingua nella scuola elementare: Risultati di alcuni test (Influence of Attention, Memory, and Phonetic Discrimination in Second Language Learning in Elementary School: Results of Several Tests).

    ERIC Educational Resources Information Center

    Colonna-Preti, Paola; Taeschner, Traute

    1987-01-01

    Using a new method, 48 children in an elementary school in Rome, Italy, were taught a foreign language (26 English, 22 German) and tested after three years. The authors attempt to explain the variation in test results in terms of the students' attention, memory, and phonetic discrimination. (CFM)

  2. Development of decision support system for oil spill management in the Mediterranean Sea

    NASA Astrophysics Data System (ADS)

    Liubartseva, Svitlana; Coppini, Giovanni; Pinardi, Nadia; De Dominicis, Michela; Marra, Palmalisa; Lecci, Rita; Turrisi, Giuseppe; Creti, Sergio; Martinelli, Sara; Agostini, Paola; Palermo, Francesco

    2016-04-01

    developed as a part of TESSA Project portfolio providing the unified access to others services. Thus, SEACONDITIONS (http://www.sea-conditions.com) performs visualization and on-line delivery of forecast of surface currents, sea surface temperature, significant wave height and direction, wave period and direction; air temperature, surface pressure, precipitation, cloud coverage, wind speed, etc. Apart from the basin scale visualization SEACONDITIONS supports the zooming capability. User feedback reports from fishermen, port authorities including Coast Guard, offshore companies, aquatic and coastal tourism managers, and academia have been collected and used for the system improvements. User-friendliness of GUI, tooltips, an opportunity to vary the advanced parameters, efficiency of the visualization tool, and a help section were appreciated in these reports. In accordance with the users' requirements, a to-do list is composed for the further development of WITOIL. This work was performed in the framework of the TESSA Project (Sviluppo di TEcnologie per la Situational Sea Awareness) supported by PON (Ricerca & Competitività 2007-2013) cofunded by UE (Fondo Europeo di sviluppo regionale), MIUR (Ministero Italiano dell'Università e della Ricerca), and MSE (Ministero dello Sviluppo Economico). References De Dominicis, M., Pinardi, N., Zodiatis, G., and Lardner, R., 2013. MEDSLIK-II, a Lagrangian marine surface oil spill model for short term forecasting - Part 1: Theory. Geosci. Model Dev. 6, 1851-1869.

  3. Sintassi e tassonomia: Teoria della valenza e lessico-grammatica in tedesco e in italiano (Syntax and Taxonomy: Theory of Valence and Lexical Grammar in German and Italian).

    ERIC Educational Resources Information Center

    Bianco, Maria Teresa

    1986-01-01

    Briefly discusses the concepts of "subject,""complement,""transitivity," and "intransitivity," contrasts the classes of complements in German and Italian based on identical criteria of classification, justifies such a classification for complements, and suggests didactic implications for the theory of valence. (CFM)

  4. Insegnamento della lingua seconda (italiano) nella scuola primaria: l'esperienza altoatesina (Teaching Italian as a Second Language in Elementary School: The Upper Adige Experience).

    ERIC Educational Resources Information Center

    Bertocchi, Daniela

    1979-01-01

    Provides an evaluation of a 30-year experience in teaching Italian to German-speaking elementary school children in an upper northeast region of Italy. Analyzes degrees of bilingualism and sociocultural contact, emphasizing the need for culturally oriented, carefully structured materials and instruction by specially trained teachers. (MES)

  5. The "Convitti Scuola della Rinascita" (The Boarding Schools of Rebirth): An Innovative Pedagogy for Democracy in Post-War Italy (1945-1955)

    ERIC Educational Resources Information Center

    Pruneri, Fabio

    2016-01-01

    In 1945, Italian society was in crisis. Twenty years of fascism and the aerial bombing and military offensives of the Second World War had left the civilian population suffering and the nation in need of reinvention. In this difficult context, certain anti-fascist intellectuals devised the interesting pedagogical experiment of the 11 Convitti…

  6. Mutual Phenomena between Galilean Satellites: PHEMU09 Campaign in Italy. (Italian Title: Occultazioni ed eclissi tra i satelliti medicei: i risultati della campagna PHEMU09 in Italia)

    NASA Astrophysics Data System (ADS)

    Marino, G.; Arena, C.; Barbieri, L.; Lopresti, C.; Lo Savio, E.; Marchini, A.; Masi, G.; Maulella, R.; Napoli, C.; Salvaggio, F.; Sbarufatti, G.; Sciuto, C.; Ruocco, N.; Ruisi, R.; Sofia, A.; Tontodonati, E.; Zambelli, R.

    2011-06-01

    Results of photometry of mutual phenomena between Galilean satellites are presented. The observations were performed in Italy during the international campaign PHEMU09.Relevant deviations (O-C) with respect to the expected central instants are present for many events. Taking into account the relative satellites’ longitudinal velocity, longitudinal discrepancies in km (and arcsec) are also computed. The discrepancies with respect to the expected flux drops during the occultations are apparently uncorrelated with the O-C on timing (and longitudinal deviations in km); moreover, for eclipses they seem greater than for occultations

  7. Guida Didattica per l'Insegnamento della Matematica. Livelli G, H = Mathematics Curriculum Guide for Italian-Speaking Students, Levels G and H. Working Draft.

    ERIC Educational Resources Information Center

    Chicago Board of Education, IL. Dept. of Curriculum.

    The curriculum guide is for levels G and H of the mathematics segment of Chicago's bilingual education program for limited-English-speaking native Italian-speaking students. It includes specific instructional objectives and worksheets for teaching the mathematical concepts of place value, whole numbers, rational numbers, measurement, geometry, and…

  8. Rapporto tra sviluppo cognitivo e competenza sintattica: Ricerca sul rapporto tra variabili cognitive e complessita sintattica in bambini di scuola elementare (Relationship between Cognitive Development and Syntactic Competence: Research on the Relationship between Cognitive Variables and Syntactic Complexity in Elementary School Children).

    ERIC Educational Resources Information Center

    Bellachi, C.; Benelli, B.

    1988-01-01

    Explains the relationship between understanding a temporal or causal event and the ability to articulate and comprehend sentences about the event. The subjects of the research were 60 children from an Italian elementary school, 20 each from the first, third, and fifth grades. (CFM)

  9. A multi-disciplinary approach for the structural monitoring of Cultural Heritages in a seismic area

    NASA Astrophysics Data System (ADS)

    Fabrizia Buongiorno, Maria; Musacchio, Massimo; Guerra, Ignazio; Porco, Giacinto; Stramondo, Salvatore; Casula, Giuseppe; Caserta, Arrigo; Speranza, Fabio; Doumaz, Fawzi; Giovanna Bianchi, Maria; Luzi, Guido; Ilaria Pannaccione Apa, Maria; Montuori, Antonio; Gaudiosi, Iolanda; Vecchio, Antonio; Gervasi, Anna; Bonali, Elena; Romano, Dolores; Falcone, Sergio; La Piana, Carmelo

    2014-05-01

    , synthetic aperture radar, optical, multispectral and panchromatic measurements), static and dynamic structural health monitoring analysis (e.g. screening tests with georadar, sonic instruments, sclerometers and optic fibers). The final purpose of the proposed approach is the development of an investigation methodology for short- and long-term Cultural Heritages preservation in response to seismic stress, which has specific features of scalability, modularity and exportability for every possible monitoring configuration. Moreover, it allows gathering useful information to furnish guidelines for Institution and local Administration to plan consolidation actions and therefore prevention activity. Some preliminary results will be presented for the test site of Calabria Region, where some architectural heritages have been properly selected as case studies for monitoring purposes. *The present work is supported and funded by Ministero dell'Università, dell'Istruzione e della Ricerca (MIUR) under the research project PON01-02710 "MASSIMO" - "Monitoraggio in Area Sismica di Sistemi Monumentali".

  10. Remote sensing and GIS for the modeling of persistent organic pollutant in the marine environment

    NASA Astrophysics Data System (ADS)

    Costanzini, S.; Teggi, S.; Bigi, A.; Ghermandi, G.

    2014-10-01

    The characterization of the marine environment plays an important role in the understanding of the dynamics affecting the transport, fate and persistence (TFP) of Persistent Organic Pollutants (POPs). This work is part of a project funded by the Ministero dell'Istruzione, dell'Università e della Ricerca. The aim of the project is the assessment of the TFP of POPs in the Mediterranean sea. The analysis will be carried out at regionalmesoscale (central Mediterranean), and at local spatial scale considering different Italian test sites (the Delta of the Po River, the Venice Lagoon and the estuary of the Rio Nocella). The first step of this work involves the implementation of GIS geodatabases for the definition of the input dataset. The geodatabases were populated with MERIS and MODIS level 2 and level 3 products of Chlorophyll-a (CHL-a), Chromophoric Dissolved Organic Matter (CDOM), Aerosol Optical Thickness (AOT), Diffuse Attenuation Coefficient (DAC), Particulate Inorganic Carbon (PIC), Particulate Organic Carbon (POC) and Sea Surface Temperature (SST). The spatial scale (central Mediterranean sea) and the reference system (Plate Carrée projection) have been imposed as a constraint for the geodatabases. Four geodatabases have been implemented, two for MODIS and two for MERIS products with a monthly, seasonal and climatological temporal scale (2002 -2013). Here, we present a first application of a methodology aimed to identify vulnerable areas to POPs accumulation and persistence. The methodology allowed to assess the spatial distribution of the CHL-a in the central Mediterranean sea. The chlorophyll concentration is related to the amount of nutrients in the water and therefore provides an indicator of the potential presence of POPs. A pilot area of 300 x 200 km located in the North Adriatic sea has been initially considered. The seasonal and climatological MODIS and MERIS CHL-a variability were retrieved and compared with in-situ forcing parameters, i.e. Po River

  11. Sintassi e Tassonomia: Teoria della valenza e lessico-grammatica in tedesco e in italiano (III) (Syntax and Taxonomy: Theory of Valence and Lexical Grammar in German and Italian, Part 3).

    ERIC Educational Resources Information Center

    Bianco, Maria Teresa

    1987-01-01

    In this article, the author concludes a three-part series in which she analyzes verb complements in German and Italian. Parts 1 and 2 of the series appear in volume 18, numbers 2 and 3, respectively. (CFM)

  12. Dall'italo-austrliano all'italiano: apprendmento linguistico fra gli scolari della seconda generazione (From Italian-Australian to Italian: Language Acquisition among the Students of the Second Generation).

    ERIC Educational Resources Information Center

    Rubino, Antonia

    1989-01-01

    Discusses the results of research to determine the success of the efforts currently being made in schools to teach pure Italian to the second generation of Italians in Australia in order to replace Italian Australian, a mixture of Italian dialects, Italian, and English. 33 references. (CFM)

  13. Studiar L'inglese dalle elementari--Problema europeo: Una proposta nuova della pedagogia cibernetica (Beginning the Study of English in the Elementary School Grades--A European Problem: A New Proposal from Cibernetic Education).

    ERIC Educational Resources Information Center

    Chiti-Batelli, Andrea

    1986-01-01

    Warns the European community of the dangers of accepting English as the international language and urges the adoption of Esperanto, a neutral language without its own cultural tradition, as the international lingua franca. (CFM)

  14. "Una Communita" Poco Nota della Communicazione non Verbale: Come Communicano il Direttore e l'Orchestra (A Little Known "Community" of Nonverbal Communication: How the Conductor and Orchestra Communicate).

    ERIC Educational Resources Information Center

    Leonardi, Magda Farago

    1992-01-01

    Examines how conductors and orchestras communicate. Communication is essentially nonverbal. Conductors use gestures, gaze, facial expression, nods and posture to deliver their message. Norbert Weiner's Cybernetic Theory of human communication is seen as a means of analyzing precisely how this musical communication takes place. (16 references) (LET)

  15. L'istituzione della funzione pragmatica esterna nel modello traduttivo in tre fasi a trasferimento indiretto (Supplying an External Pragmatic Purpose to a Three-Stage Translation Model with Indirect Transfer).

    ERIC Educational Resources Information Center

    Horrakh, Livio

    1989-01-01

    Discusses a didactic model for translation based on textual linguistics that incorporates a model second external reader in the translation process. Although translation is necessarily sentence oriented, this method considers the entire text that must eventually satisfy a pragmatic external purpose. (51 references) (CFM)

  16. Presupposti teorici per un progetto di insegnamento della traduzione dalla lingua straniera basato sull'analisi testuale (Theoretical Presuppositions for a Project to Teach Translation from a Foreign Language Based on Textual Analysis).

    ERIC Educational Resources Information Center

    Horrakh, Livio

    1987-01-01

    Discusses an approach to translation based on textual linguistics. The student/translator first looks at the foreign language text as a whole, then analyzes its parts, and finally arrives at a new synthesis (the translation) that is comparable to the original text. (CFM)

  17. La teoria dell'attivita verbale nella fase attuale e il suo significato nell'insegnamento della lingua russa agli stranieri (The Theory of Verbal Activity in its Present Stage and its Significance in the Teaching of Russian to Foreigners)

    ERIC Educational Resources Information Center

    Leont'ev, A. A.

    1977-01-01

    Traces the evolution of thought in the field of psycholinguistics ("theory of verbal activity" in Russia) and discusses current research being done in the United States, Europe, and the Soviet Union. A valid method of teaching language cannot be developed without a theory of language acquisition. (Text is in Italian.) (CFM)

  18. Serum cholesterol and acute myocardial infarction: a case-control study from the GISSI-2 trial. Gruppo Italiano per lo Studio della Sopravvivenza nell'Infarto-Epidemiologia dei Fattori di Rischio dell'Infarto Miocardico Investigators.

    PubMed Central

    Nobili, A.; D'Avanzo, B.; Santoro, L.; Ventura, G.; Todesco, P.; La Vecchia, C.

    1994-01-01

    OBJECTIVE--To examine the role of serum cholesterol in acute myocardial infarction in a population of patients with no history of coronary heart disease and to establish the nature of this association, the degree of risk, and the possible interaction between serum cholesterol and other major risk factors for acute myocardial infarction. DESIGN--Case-control study. SETTING--90 hospitals in northern, central, and southern Italy. PATIENTS--916 consecutive cases of newly diagnosed acute myocardial infarction and 1106 hospital controls admitted to hospital with acute conditions not related to known or suspected risk factors for coronary heart disease. DATA COLLECTION--Data were collected with a structured questionnaire and blood samples were taken by venepuncture as soon as possible after admission to hospital from cases and controls. Blood cholesterol concentrations were available for 614 cases and 792 controls. RESULTS--After adjustment by logistic regression for sex, age, education, geographical area, smoking status, body mass index, history of diabetes and hypertension, and family history of coronary heart disease the estimated relative risks of acute myocardial infarction for quintiles of serum cholesterol (from lowest to highest) were 2.3 (95% confidence interval (CI) 1.6 to 3.4), 3.1 (95% CI 2.1 to 4.6), 4.1 (95% CI 2.8 to 6.0), and 5.2 (95% CI 3.5 to 7.7). The estimated relative risk across selected covariates increased from the lowest to the highest quintile of serum cholesterol particularly for men, patients under 55 years of age, and smokers. When the possible interaction of known risk factors with serum cholesterol was examined, smoking habits, diabetes, and hypertension had approximately multiplicative effects on relative risk. CONCLUSIONS--This study indicates that serum cholesterol was an independent risk factor for acute myocardial infarction. This association was linear, with no threshold level. Moreover, there was a multiplicative effect between cholesterol and other major risk factors on the relative risk of acute myocardial infarction. PMID:8011413

  19. PREFACE: DICE 2012 : Spacetime Matter Quantum Mechanics - from the Planck scale to emergent phenomena

    NASA Astrophysics Data System (ADS)

    Diósi, Lajos; Elze, Hans-Thomas; Fronzoni, Leone; Halliwell, Jonathan; Prati, Enrico; Vitiello, Giuseppe; Yearsley, James

    2013-06-01

    Presented in this volume are the Invited Lectures and the Contributed Papers of the Sixth International Workshop on Decoherence, Information, Complexity and Entropy - DICE 2012, held at Castello Pasquini, Castiglioncello (Tuscany), 17-21 September 2012. These proceedings may document to the interested public and to the wider scientific community the stimulating exchange of ideas at the meeting. The number of participants has been steadily growing over the years, reflecting an increasing attraction, if not need, of such conference. Our very intention has always been to bring together leading researchers, advanced students, and renowned scholars from various areas, in order to stimulate new ideas and their exchange across the borders of specialization. In this way, the series of meetings successfully continued from the beginning with DICE 20021, followed by DICE 20042, DICE 20063, DICE 20084, and DICE 20105, Most recently, DICE 2012 brought together more than 120 participants representing more than 30 countries worldwide. It has been a great honour and inspiration to have Professor Yakir Aharonov (Tel Aviv) with us, who presented the opening Keynote Lecture 'The two-vector quantum formalism'. With the overarching theme 'Spacetime - Matter - Quantum Mechanics - from the Planck scale to emergent phenomena', the conference took place in the very pleasant and inspiring atmosphere of Castello Pasquini - in beautiful surroundings, overlooking a piece of Tuscany's coast. The 5-day program covered these major topics: Quantum Mechanics, Foundations and Quantum-Classical Border Quantum-Classical Hybrids and Many-Body Systems Spectral Geometry, Path Integrals and Experiments Quantum -/- Gravity -/- Spacetime Quantum Mechanics on all Scales? A Roundtable Discussion under the theme 'Nuovi orizzonti nella ricerca scientifica. Ci troviamo di fronte ad una rivoluzione scientifica?' formed an integral part of the program. With participation of E Del Giudice (INFN & Università di

  20. Wild 2 grains characterized combining MIR/FIR/Raman micro-spectroscopy and FE-SEM/EDS analyses

    NASA Astrophysics Data System (ADS)

    Ferrari, M.; Rotundi, A.; Rietmeijer, F. J. M.; Della Corte, V.; Baratta, G. A.; Brunetto, R.; Dartois, E.; Djouadi, Z.; Merouane, S.; Borg, J.; Brucato, J. R.; Le Sergeant d'Hendecourt, L.; Mennella, V.; Palumbo, M. E.; Palumbo, P.

    and, if so, which fraction of the dust in these comets is truly represented by non-processed silicates and organic material. The work done for Stardust samples is important to understand the similarities and differences among comets. In fact, the results of this study are relevant also for the ROSETTA mission that encountered the Jupiter-Family (J-F) comet 67P/Churyumov-Gerasimenko in August, 2014. At the time this mission was launched, our ideas of comet dust were biased by the findings of the Halley missions. The Stardust mission showed an unexpected richness of dust that originated from the inner solar system. Rosetta is confirming these results but also adding information, in particular on the presence of a primitive and unprocessed dust component \\cite{Fulle15}. The work was supported by PRIN2008/MIUR (Ministero dell'Istruzione dell'Università e della Ricerca), the Italian Space Agency (ASI), and MAE (Ministero degli Affari Esteri). The IAS team is grateful to the French space agency CNES for funding and supporting this work as well as to the CNRS PNP planetology program. FJMR was supported by grant NNX11AC36G through the NASA LARS Program. We thank the NASA Johnson Space Center/Astromaterials Curation laboratory for providing the samples.

  1. A S.M.A.R.T. system for the seismic vulnerability mitigation of Cultural Heritages

    NASA Astrophysics Data System (ADS)

    Montuori, Antonio; Costanzo, Antonio; Gaudiosi, Iolanda; Vecchio, Antonio; Minasi, Mario; Falcone, Sergio; La Piana, Carmelo; Stramondo, Salvatore; Casula, Giuseppe; Giovanna Bianchi, Maria; Fabrizia Buongiorno, Maria; Musacchio, Massimo; Doumaz, Fawzi; Ilaria Pannaccione Apa, Maria

    2016-04-01

    enable assessing the site seismic effects, the built-up structural features and the surface deformation processes of the local-scale area on a monthly temporal span; c) the real- to near-real-time and building scale analysis of the heritage through proximal remotely sensing tools (e.g. terrestrial laser scanning, infrared thermal cameras and real aperture radar), combined with ambient vibration tests. They allow analyzing geometric, structural and material properties / anomalies of buildings as well as the state of conservation of structures on a real-time temporal span. The proposed approach is: Specific (it targets the cultural heritages monitoring for seismic mitigation purposes); Measurable (it provides synthetic descriptors or maps able to quantify structural and the environmental properties / anomalies / trends); Action-oriented (it provides information to plan consolidation and restoration actions for prevention activity); Relevant (it allows achieving consolidated results for cultural heritage monitoring); Time-related (it specifies when the results can be achieved). Meaningful results, obtained for the Saint Augustine Complex (XVI century) located in the historic center of the Calabrian chief town of Cosenza, are presented in terms of a web-based Geographic Information System (GIS) platform and a 3-dimensional (3D) visual software for the monitoring of environmental/urban landscapes and buildings. These tools represent the added-value products of the proposed SMART system, which allow integrating and combining multi-sensors analyses in order to support end-users involved into a cultural heritage monitoring. * The present work is supported and funded by Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR) under the research project PON01-02710 "MASSIMO" - "Monitoraggio in Area Sismica di Sistemi Monumentali".

  2. Functional characterization and developmental expression profiling of gibberellin signalling components in Vitis vinifera

    PubMed Central

    Acheampong, Atiako Kwame; Hu, Jianhong; Rotman, Ariel; Zheng, Chuanlin; Halaly, Tamar; Takebayashi, Yumiko; Jikumaru, Yusuke; Kamiya, Yuji; Lichter, Amnon; Sun, Tai-Ping; Or, Etti

    2015-01-01

    Gibberellins (GAs) regulate numerous developmental processes in grapevine (Vitis vinifera) such as rachis elongation, fruit set, and fruitlet abscission. The ability of GA to promote berry enlargement has led to its indispensable use in the sternospermocarpic (‘seedless’) table grape industry worldwide. However, apart from VvGAI1 (VvDELLA1), which regulates internode elongation and fruitfulness, but not berry size of seeded cultivars, little was known about GA signalling in grapevine. We have identified and characterized two additional DELLAs (VvDELLA2 and VvDELLA3), two GA receptors (VvGID1a and VvGID1b), and two GA-specific F-box proteins (VvSLY1a and VvSLY1b), in cv. Thompson seedless. With the exception of VvDELLA3-VvGID1b, all VvDELLAs interacted with the VvGID1s in a GA-dependent manner in yeast two-hybrid assays. Additionally, expression of these grape genes in corresponding Arabidopsis mutants confirmed their functions in planta. Spatiotemporal analysis of VvDELLAs showed that both VvDELLA1 and VvDELLA2 are abundant in most tissues, except in developing fruit where VvDELLA2 is uniquely expressed at high levels, suggesting a key role in fruit development. Our results further suggest that differential organ responses to exogenous GA depend on the levels of VvDELLA proteins and endogenous bioactive GAs. Understanding this interaction will allow better manipulation of GA signalling in grapevine. PMID:25588745

  3. GA signalling and cross-talk with other signalling pathways.

    PubMed

    Lor, Vai S; Olszewski, Neil E

    2015-01-01

    Gibberellins (GAs) are phytohormones that regulate growth and development. DELLA proteins repress GA responses. GA binding to its receptor triggers a series of events that culminate in the destruction of DELLA proteins by the 26S proteasome, which removes the repression of GA signalling. DELLA proteins are transcription co-activators that induce the expression of genes which encode products that inhibit GA responses. In addition to repressing GA responses, DELLA proteins influence the activity of other signalling pathways and serve as a central hub from which other pathways influence GA signalling. In this role, DELLA proteins bind to and inhibit proteins, including transcription factors that act in the signalling pathways of other hormones and light. The binding of these proteins to DELLA proteins also inhibits DELLA activity. GA signalling is subject to homoeostatic regulation through GA-induced repression of GA biosynthesis gene expression, and increased production of the GA receptor and enzymes that catabolize bioactive GAs. This review also discusses the nature of mutant DELLA alleles that are used to produce high-yielding 'Green Revolution' cereal varieties, and highlights important gaps in our knowledge of GA signalling. PMID:26374886

  4. A COMPARATIVE STUDY OF TWO F-BOX PROTEINS, SLEEPY1 AND SNEEZY IN GA SIGNALING

    Technology Transfer Automated Retrieval System (TEKTRAN)

    SNEEZY (SNE) is a homolog of SLEEPY1 (SLY1), encoding an F-box protein subunit of an SCF E3 ubiquitin ligase complex in Arabidopsis. SLY1 plays a central role in destruction of DELLA negative family proteins via 26S proteasome pathway in GA signaling pathway. DELLA proteins consist of five memb...

  5. Gibberellin Perception by the Gibberellin Receptor and its Effector Recognition

    NASA Astrophysics Data System (ADS)

    Hakoshima, Toshio; Murase, Kohji; Hirano, Yoshinori; Sun, Tai-Ping

    Gibberellins control a diverse range of growth and developmental processes in higher plants and have been widely utilized in the agricultural industry. By binding to a nuclear receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1), gibberellins regulate gene expression by promoting degradation of the transcriptional regulator DELLA proteins. The precise manner in which GID1 discriminates and becomes activated by bioactive gibberellins for specific binding to DELLA proteins remains unclear. We present the crystal structure of a ternary complex of Arabidopsis thaliana GID1A, a bioactive gibberellin and the N-terminal DELLA domain of GAI. In this complex, GID1a occludes gibberellin in a deep binding pocket covered by its N-terminal helical switch region, which in turn interacts with the DELLA domain containing DELLA, VHYNP and LExLE motifs. Our results establish a structural model of a plant hormone receptor which is distinct from the hormone-perception mechanism and effector recognition of the known auxin receptors.

  6. Gibberellin signaling.

    PubMed

    Hartweck, Lynn M

    2008-12-01

    This review covers recent advances in gibberellin (GA) signaling. GA signaling is now understood to hinge on DELLA proteins. DELLAs negatively regulate GA response by activating the promoters of several genes including Xerico, which upregulates the abscisic acid pathway which is antagonistic to GA. DELLAs also promote transcription of the GA receptor, GIBBERELLIN INSENSITIVE DWARF 1 (GID1) and indirectly regulate GA biosynthesis genes enhancing GA responsiveness and feedback control. A structural analysis of GID1 provides a model for understanding GA signaling. GA binds within a pocket of GID1, changes GID1 conformation and increases the affinity of GID1 for DELLA proteins. GA/GID1/DELLA has increased affinity for an F-Box protein and DELLAs are subsequently degraded via the proteasome. Therefore, GA induces growth through degradation of the DELLAs. The binding of DELLA proteins to three of the PHYTOCHROME INTERACTING FACTOR (PIF) proteins integrates light and GA signaling pathways. This binding prevents PIFs 3, 4, and 5 from functioning as positive transcriptional regulators of growth in the dark. Since PIFs are degraded in light, these PIFs can only function in the combined absence of light and presence of GA. New analyses suggest that GA signaling evolved at the same time or just after the plant vascular system and before plants acquired the capacity for seed reproduction. An analysis of sequences cloned from Physcomitrella suggests that GID1 and DELLAs were the first to evolve but did not initially interact. The more recently diverging spike moss Selaginella has all the genes required for GA biosynthesis and signaling, but the role of GA response in Selaginella physiology remains a mystery. PMID:18936962

  7. Gibberellins control fruit patterning in Arabidopsis thaliana

    PubMed Central

    Arnaud, Nicolas; Girin, Thomas; Sorefan, Karim; Fuentes, Sara; Wood, Thomas A.; Lawrenson, Tom; Sablowski, Robert; Østergaard, Lars

    2010-01-01

    The Arabidopsis basic helix–loop–helix (bHLH) proteins INDEHISCENT (IND) and ALCATRAZ (ALC) specify tissues required for fruit opening that have major roles in seed dispersal and plant domestication. Here, we show that synthesis of the phytohormone gibberellin is a direct and necessary target of IND, and that ALC interacts directly with DELLA repressors, which antagonize ALC function but are destabilized by gibberellin. Thus, the gibberellin/DELLA pathway has a key role in patterning the Arabidopsis fruit, and the interaction between DELLA and bHLH proteins, previously shown to connect gibberellin and light responses, is a versatile regulatory module also used in tissue patterning. PMID:20889713

  8. Gibberellins control fruit patterning in Arabidopsis thaliana.

    PubMed

    Arnaud, Nicolas; Girin, Thomas; Sorefan, Karim; Fuentes, Sara; Wood, Thomas A; Lawrenson, Tom; Sablowski, Robert; Østergaard, Lars

    2010-10-01

    The Arabidopsis basic helix-loop-helix (bHLH) proteins INDEHISCENT (IND) and ALCATRAZ (ALC) specify tissues required for fruit opening that have major roles in seed dispersal and plant domestication. Here, we show that synthesis of the phytohormone gibberellin is a direct and necessary target of IND, and that ALC interacts directly with DELLA repressors, which antagonize ALC function but are destabilized by gibberellin. Thus, the gibberellin/DELLA pathway has a key role in patterning the Arabidopsis fruit, and the interaction between DELLA and bHLH proteins, previously shown to connect gibberellin and light responses, is a versatile regulatory module also used in tissue patterning. PMID:20889713

  9. Flexible control of plant architecture and yield via switchable expression of Arabidopsis gai.

    PubMed

    Ait-ali, Tahar; Rands, Carley; Harberd, Nicholas P

    2003-09-01

    The growth of plants is repressed by DELLA proteins, nuclear regulators whose activities are opposed by the growth-promoting phytohormone gibberellin (GA). Mutations affecting DELLA protein function were previously used by plant breeders to create the high-yielding semidwarf wheat varieties of the green revolution. gai is an Arabidopsis mutant DELLA protein-encoding orthologue of the wheat semidwarfing genes. Here we describe the development of a transgene that confers ethanol-inducible gai expression. Transient induction of gai causes transient growth repression: growth prior to and after treatment is unaffected. Appropriate ethanol treatments result in dwarf plants that produce the same numbers of seeds as untreated controls. This new technology represents a substantial advance in the applicability of genes encoding mutant DELLA proteins to agricultural and horticultural improvement, enhancing the flexibity with which these genes can be used for the sustainable achievement of increased crop plant yields. PMID:17166132

  10. La relatività debole. La fisica dello spazio e del tempo senza paradossi

    NASA Astrophysics Data System (ADS)

    Selleri, Franco

    2011-06-01

    Secondo Einstein e Poincaré la simultaneità di eventi che hanno luogo in punti diversi dello spazio può essere definita solo per convenzione. Dal punto di vista matematico si dimostra, quindi, che due diverse definizioni di simultaneità corrispondono a due diversi valori del coefficiente e1 della variabile spaziale x presente nelle trasformazioni di Lorentz. A partire da premesse normalmente accettate otterremo diverse dimostrazioni della necessità di reintrodurre il concetto di simultaneità assoluta. Le conseguenze cosmologiche della nuova struttura dello spazio e del tempo andranno contro la cosmologia del Big Bang. Inoltre, dopo questi risultati, il relativismo sopravviverà in una forma meno aspra ("relatività debole") perché risulter privo di quel campionario di paradossi portato dalla teoria della Relatività Speciale.

  11. L'eliosismologia: onde sismiche per studiare l'interno del Sole

    NASA Astrophysics Data System (ADS)

    Di Mauro, M. P.

    2014-12-01

    Negli ultimi cinquanta anni siamo stati testimoni di una straordinaria rivoluzione della conoscenza e comprensione della nostra stella grazie alla nascita dell'Eliosismologia, lo studio delle oscillazioni solari. Analogamente a ciò che accade nella Terra durante i terremoti, anche l'interno del Sole è pervaso continuamente da onde sismiche che provocano piccole oscillazioni, ovvero deformazioni della fotosfera. Le oscillazioni sono la manifestazione di diversi processi che avvengono all'interno della struttura del Sole e le frequenze sismiche dei modi osservati e misurati sulla superficie sono legate direttamente ai parametri fisici degli strati interni attraversati dalle onde sismiche. Lo studio delle oscillazioni rappresenta, quindi, l'unico metodo diretto per studiare la struttura e la dinamica interna del Sole. In questo articolo verranno illustrate le caratteristiche generali delle oscillazioni solari e verranno presentati i risultati importanti e i progressi notevoli raggiunti grazie all'Eliosismologia.

  12. Redescription of two European species of Acerentomidae (Protura) belonging to the Italian fauna.

    PubMed

    Galli, Loris; Capurro, Matteo; Costa, Fabio; Stadio, Gabriele Di; Sarà, Antonio; Zinni, Matteo

    2016-01-01

    Acerentulus apuliacus Rusek & Stumpp, 1988 is redescribed based on new specimens collected in Apulia and Basilicata (S-Italy) and deposited in the collection of Geneva Natural History Museum. Podolinella ruseki (Nosek, 1967) new combination is redescribed, based on the holotype from Austria and additional specimens collected in Liguria, NW Italy. The new material is deposited in the collection of the Dipartimento di Scienze della Terra, dell'Ambiente e della Vita, University of Genoa. PMID:27615841

  13. [Food Addiction: definizione, misurazione e prevalenza in soggetti sani e in pazienti con disturbi del comportamento alimentare].

    PubMed

    Imperatori, Claudio; Fabbricatore, Mariantonietta; Vumbaca, Viviana; Innamorati, Marco; Contardi, Anna; Farina, Benedetto

    2016-01-01

    RIASSUNTO. Il costrutto della "Food Addiction" (FA) è stato introdotto negli ultimi anni per meglio comprendere i pattern alimentari disfunzionali osservati nei pazienti obesi e in sovrappeso e nei pazienti con disturbi del comportamento alimentare (DCA). Nonostante i numerosi parallelismi tra la dipendenza da sostanze e la FA, non c'è ancora accordo nel considerare la FA come un indipendente DCA né un concetto utile. L'obiettivo della presente rassegna è stato quello di riassumere i dati disponibili al fine di aumentare le conoscenze su: 1) definizione, misurazione e caratteristiche generali della FA; 2) prevalenza della FA in campioni clinici e non clinici. I dati disponibili suggeriscono che la FA sia un costrutto transnosografico presente in tutti i DCA, con una prevalenza più elevata nella bulimia nervosa. Nonostante il dibattito sull'autonomia della FA come DCA rimanga aperto, gli studi riportati suggeriscono che la comorbilità tra FA e DCA sia associata con peggiori outcome clinici, giustificando di conseguenza l'assessment e il trattamento della FA. PMID:27183510

  14. Tissue-Specific Regulation of Gibberellin Signaling Fine-Tunes Arabidopsis Iron-Deficiency Responses.

    PubMed

    Wild, Michael; Davière, Jean-Michel; Regnault, Thomas; Sakvarelidze-Achard, Lali; Carrera, Esther; Lopez Diaz, Isabel; Cayrel, Anne; Dubeaux, Guillaume; Vert, Grégory; Achard, Patrick

    2016-04-18

    Iron is an essential element for most living organisms. Plants acquire iron from the rhizosphere and have evolved different biochemical and developmental responses to adapt to a low-iron environment. In Arabidopsis, FIT encodes a basic helix-loop-helix transcription factor that activates the expression of iron-uptake genes in root epidermis upon iron deficiency. Here, we report that the gibberellin (GA)-signaling DELLA repressors contribute substantially in the adaptive responses to iron-deficient conditions. When iron availability decreases, DELLAs accumulate in the root meristem, thereby restraining root growth, while being progressively excluded from epidermal cells in the root differentiation zone. Such DELLA exclusion from the site of iron acquisition relieves FIT from DELLA-dependent inhibition and therefore promotes iron uptake. Consistent with this mechanism, expression of a non-GA-degradable DELLA mutant protein in root epidermis interferes with iron acquisition. Hence, spatial distribution of DELLAs in roots is essential to fine-tune the adaptive responses to iron availability. PMID:27093087

  15. La Segmentazione Grafica della Scrittura di una Storia Conosciuta. Uno Studio Descrittivo su 450 Testi di Bambini dai 6 agli 8 Anni (Graphic Segmentation of the Writing of a Familiar Story. A Descriptive Study of 450 Texts of Children from 6 to 8 Years Old).

    ERIC Educational Resources Information Center

    Pontecorvo, Clotilde; Di Eduardo, Roberta

    1995-01-01

    The purpose of this study was to analyze how children in the early grades of elementary school divide words while writing. The subjects were 450 Italian children belonging either to upper-middle-class or lower-middle-class families who were asked to write the familiar story of "Little Red Riding Hood." (CFM)

  16. PREFACE: DICE 2012 : Spacetime Matter Quantum Mechanics - from the Planck scale to emergent phenomena

    NASA Astrophysics Data System (ADS)

    Diósi, Lajos; Elze, Hans-Thomas; Fronzoni, Leone; Halliwell, Jonathan; Prati, Enrico; Vitiello, Giuseppe; Yearsley, James

    2013-06-01

    Presented in this volume are the Invited Lectures and the Contributed Papers of the Sixth International Workshop on Decoherence, Information, Complexity and Entropy - DICE 2012, held at Castello Pasquini, Castiglioncello (Tuscany), 17-21 September 2012. These proceedings may document to the interested public and to the wider scientific community the stimulating exchange of ideas at the meeting. The number of participants has been steadily growing over the years, reflecting an increasing attraction, if not need, of such conference. Our very intention has always been to bring together leading researchers, advanced students, and renowned scholars from various areas, in order to stimulate new ideas and their exchange across the borders of specialization. In this way, the series of meetings successfully continued from the beginning with DICE 20021, followed by DICE 20042, DICE 20063, DICE 20084, and DICE 20105, Most recently, DICE 2012 brought together more than 120 participants representing more than 30 countries worldwide. It has been a great honour and inspiration to have Professor Yakir Aharonov (Tel Aviv) with us, who presented the opening Keynote Lecture 'The two-vector quantum formalism'. With the overarching theme 'Spacetime - Matter - Quantum Mechanics - from the Planck scale to emergent phenomena', the conference took place in the very pleasant and inspiring atmosphere of Castello Pasquini - in beautiful surroundings, overlooking a piece of Tuscany's coast. The 5-day program covered these major topics: Quantum Mechanics, Foundations and Quantum-Classical Border Quantum-Classical Hybrids and Many-Body Systems Spectral Geometry, Path Integrals and Experiments Quantum -/- Gravity -/- Spacetime Quantum Mechanics on all Scales? A Roundtable Discussion under the theme 'Nuovi orizzonti nella ricerca scientifica. Ci troviamo di fronte ad una rivoluzione scientifica?' formed an integral part of the program. With participation of E Del Giudice (INFN & Università di

  17. A multidisciplinary environmental integrated approach to better understand the Tegnue Reefs formation, offshore Chioggia, Northern Adriatic Sea.

    NASA Astrophysics Data System (ADS)

    Bergamasco, Andrea; Donnici, Sandra; Tosi, Luigi; Tagliapietra, Davide; Zaggia, Luca; Bonaldo, Davide; Braga, Federica; Da Lio, Cristina; Keppel, Erica; Lorenzetti, Giuliano; Manfè, Giorgia; Franceschini, Gianluca; Giovanardi, Otello; Carol, Eleonora; Fornaro, Elena; Grant, Carley

    2014-05-01

    , and benthic invertebrates like bryozoans, mollusks, serpulids and corals also contribute to the final building of the status of Tegnue's environment, but we want to demonstrate that the in first stage of consolidation abiotic phenomena could have played a major role. In order to prove our thesis a series of ground water sampling as well as rock sampling and sediment cores were carried out. The poster illustrates the overall ongoing program as well as some work in progress and first results from field cruise. Acknowledgments This work was supported by the Municipality of Chioggia through the Tegnue Project and partially by the Flagship Project RITMARE - The Italian Research for the Sea - coordinated by the Italian National Research Council and funded by the Italian Ministry of Education, University and Research within the National Research Program 2011-2013, and by the Scientific Cooperation Agreement between the CONICET (Argentina) - CNR (Italy), Program 2013-2014, Project 'Fresh-saltwaters in high-value coastlands: from the hydrogeophysical/geochemical characterization of the present interactions to the modeling quantification of the expected effects of climate changes'. References - Bonardi, M., Tosi, L. 2000. Indagini preliminari su alcune formazioni di sabbie cementate nella laguna di Venezia. In: La Ricerca Scientifica Per Venezia. Il Progetto Sistema Lagunare Veneziano, Modellistica del Sistema Lagunare Studio di Impatto Ambientale. Istituto Veneto di Scienze, Lettere ed Arti, Vol. II, Tomo II, p. 967-974, Venezia, ISBN: 88-86166-82-6. - Bonardi, M., Tosi, L., Rizzetto, F., Brancolini, G., Baradello, L. 2006. Effects of climate changes on the late Pleistocene and Holocene sediments of the Venice Lagoon, Italy. Journal of Coastal Research, SI 39, 279-284. - Casellato S., Stefanon A. 2008. Coralligenous habitat in the northern Adriatic Sea: an overview. Marine Ecology, 29: 321-341. - Gabbianelli, G., Colantoni, P., Degetto, S., Dinelli, E., Lucchini, F. 1997

  18. Specialised teams or personal continuity across inpatient and outpatient mental healthcare? Study protocol for a natural experiment

    PubMed Central

    Giacco, Domenico; Bird, Victoria Jane; McCrone, Paul; Lorant, Vincent; Nicaise, Pablo; Pfennig, Andrea; Bauer, Michael; Ruggeri, Mirella; Lasalvia, Antonio; Moskalewicz, Jacek; Welbel, Marta; Priebe, Stefan

    2015-01-01

    Introduction Mental healthcare organisation can either pursue specialisation, that is, distinct clinicians and teams for inpatient and outpatient care or personal continuity of care, that is, the same primary clinician for a patient across the two settings. Little systematic research has compared these approaches. Existing studies subject have serious methodological shortcomings. Yet, costly reorganisations of services have been carried out in different European countries, inconsistently aiming to achieve specialisation or personal continuity of care. More reliable evidence is required on whether specialisation or continuity of care is more effective and cost-effective, and whether this varies for different patient groups and contexts. Design and methods In a natural experiment, we aim to recruit at least 6000 patients consecutively admitted to inpatient psychiatric care in Belgium, Germany, Italy, Poland, and the UK. In each country, care approaches supporting specialisation and personal continuity coexist. Patients will be followed up at 1 year to compare outcomes, costs and experiences. Inclusion criteria are: 18 years of age or older; clinical diagnosis of psychosis, affective disorder or anxiety/somatisation disorder; sufficient command of the language of the host country; absence of cognitive deterioration and/or organic brain disorders; and capacity to provide informed consent. Ethics and dissemination Ethical approval was obtained in all countries: (1) England: NRES Committee North East—Newcastle & North Tyneside (ref: 14/NE/1017); (2) Belgium: Comité d'Ethique hospitalo-facultaire des Cliniques St-Luc; (3) Germany: Ethical Board, Technische Universität Dresden; (4) Italy: Comitati Etici per la sperimentazione clinica (CESC) delle provincie di Verona, Rovigo, Vicenza, Treviso, Padova; (5) Poland: Komisja Bioetyczna przy Instytucie Psychiatrii i Neurologii w Warszawie. We will disseminate the findings through scientific publications and a study

  19. Identification and characterization of Arabidopsis gibberellin receptors.

    PubMed

    Nakajima, Masatoshi; Shimada, Asako; Takashi, Yoshiyuki; Kim, Young-Cheon; Park, Seung-Hyun; Ueguchi-Tanaka, Miyako; Suzuki, Hiroyuki; Katoh, Etsuko; Iuchi, Satoshi; Kobayashi, Masatomo; Maeda, Tatsuya; Matsuoka, Makoto; Yamaguchi, Isomaro

    2006-06-01

    Three gibberellin (GA) receptor genes (AtGID1a, AtGID1b and AtGID1c), each an ortholog of the rice GA receptor gene (OsGID1), were cloned from Arabidopsis, and the characteristics of their recombinant proteins were examined. The GA-binding activities of the three recombinant proteins were confirmed by an in vitro assay. Biochemical analyses revealed similar ligand selectivity among the recombinants, and all recombinants showed higher affinity to GA(4) than to other GAs. AtGID1b was unique in its binding affinity to GA(4) and in its pH dependence when compared with the other two, by only showing binding in a narrow pH range (pH 6.4-7.5) with 10-fold higher affinity (apparent K(d) for GA(4) = 3 x 10(-8) m) than AtGID1a and AtGID1c. A two-hybrid yeast system only showed in vivo interaction in the presence of GA(4) between each AtGID1 and the Arabidopsis DELLA proteins (AtDELLAs), negative regulators of GA signaling. For this interaction with AtDELLAs, AtGID1b required only one-tenth of the amount of GA(4) that was necessary for interaction between the other AtGID1s and AtDELLAs, reflecting its lower K(d) value. AtDELLA boosted the GA-binding activity of AtGID1 in vitro, which suggests the formation of a complex between AtDELLA and AtGID1-GA that binds AtGID1 to GA more tightly. The expression of each AtGID1 clone in the rice gid1-1 mutant rescued the GA-insensitive dwarf phenotype. These results demonstrate that all three AtGID1s functioned as GA receptors in Arabidopsis. PMID:16709201

  20. Hypogenic contribution to speleogenesis in a predominant epigenic karst system: A case study from the Venetian Alps, Italy

    NASA Astrophysics Data System (ADS)

    Tisato, Nicola; Sauro, Francesco; Bernasconi, Stefano M.; Bruijn, Rolf H. C.; De Waele, Jo

    2012-05-01

    Buso della Rana and Buso della Pisatela are two karstic caves located in north-east Italy. They are part of the same karst system and are developed in the Castelgomberto calcarenitic marine sediments, which were deposited in a shallow Caribbean-type sea during the Eocene. The Buso della Rana-Pisatela system developed mostly at the contact between the Castelgomberto calcarenite and underlying volcanic rocks. The system of caves is ~ 37 km long and has only three entrances, two of which are semi-artificial. The overlying karst plateau is not directly connected to the Buso della Rana-Pisatela system and, with the exception of one deep abyss, exhibits a rather poorly developed karst. This is unexpected considering the presence at depth of such a large and long cave. Gypsum (CaSO4·2H2O) has locally been observed on the walls of the Buso della Pisatela cave. Energy dispersive X-ray spectroscopy (EDS), performed with a scanning electron microscope (SEM), reveals the presence of sulfur-bearing minerals within the host rock. Gypsum was formed by oxidation of these minerals as indicated by negative δ34S values. The oxidation of sulfide minerals forms a sulfuric-acid solution that dissolves the Castelgomberto calcarenite and, once it is oversaturated in calcium, precipitates as gypsum. The lack of well-developed karst on top of the plateau and the analyses suggest that the formation mechanisms for the Buso della Rana-Pisatela system differ from classical epigenic speleogenesis. The "pyrite-effect" has been recognized in other caves and described in literature. In our case pyrite is responsible of two hypo-speleogenetic processes: i) the dissolution of a portion of the host rock and ii) the enlargement of the karst voids as a consequence of the haloclastic effect.

  1. Optimierung und Charakterisierung einer transportablen optischen Gitteruhr mit Ytterbium

    NASA Astrophysics Data System (ADS)

    Mura, Gregor

    2015-08-01

    This thesis deals with the improvement and the characterization of a transportable optical lattice clock with ytterbium. Building on results of former theses from T. Franzen [1] and C. Abou Jadoudeh [2] numerous improvements were needed to increase the overall stability of the whole apparatus. The improved stability should lead to a more accurate characterization of the system and a better mobility. Finally, the apparatus should be transported to the Instituto Nationale di Ricerca Metrologica (INRIM) in Torino (Italy) in the course of the Space Optical Clocks 2 (SOC2) project.(Abridged)

  2. Allocation of Attention and Effect of Practice on Persons with and without Mental Retardation

    ERIC Educational Resources Information Center

    Oka, Kohei; Miura, Toshiaki

    2008-01-01

    Persons with mild and moderate mental retardation and CA-matched persons without mental retardation performed a dual-task, "pencil-and-paper task" (Baddeley, Della Sala, Gray, Papagno, & Spinnler (1997). Testing central executive functioning with a pencil-and-paper test. In Rabbit (Ed.), Methodology of Frontal and Executive Function (pp. 61-80).…

  3. The Urban Complex in Cattolica, Italy.

    ERIC Educational Resources Information Center

    PEB Exchange, 2003

    2003-01-01

    The Italian city of Cattolica has developed an urban complex, the Piazza della Repubblica, that offers a wide range of public services. In renovated facilities it provides a modern architectural setting based on the idea of a traditional town square. It houses a primary school, cultural center (including a library), and theater, and it is an…

  4. Romans 12 Motivational Gifts and College Professors: Implications for Job Satisfaction and Person-Job Fit

    ERIC Educational Resources Information Center

    Tomlinson, Jon C.; Winston, Bruce E.

    2011-01-01

    This study builds on earlier work by DellaVecchio and Winston (2004) and McPherson (2008). They addressed the seven motivational gifts Paul wrote about in Romans 12:3-8 as a means for addressing job satisfaction and person-job fit among college professors. Using a snowball sampling method, 89 college professors completed the online survey…

  5. Invitation to Lifelong Learning.

    ERIC Educational Resources Information Center

    Gross, Ronald, Ed.

    In this anthology of readings, 28 scholars and educators consider the theoretical and practical forces that shape the concept of lifelong learning. After Ronald Gross's introduction, the first section begins its consideration of the Western tradition in adult learning with Plato's metaphor of the cave and includes Pico della Mirandola's essay on…

  6. Mutations in the F-box gene SNEEZY result in decreased arabidopsis GA signaling

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We previously reported that the SLEEPY1 (SLY1) homolog, F-box gene SNEEZY/SLEEPY2 (SNE/SLY2), can partly replace SLY1 in gibberellin (GA) hormone signaling through interaction with DELLAs RGA and GAI. To determine whether SNE normally functions in GA signaling, we characterized the phenotypes of tw...

  7. The Emergence of For-Profit E-learning Providers in Asia

    ERIC Educational Resources Information Center

    Perkins, Ross

    2009-01-01

    This article presents an interview with Dr. Luca Botturi, winner of the 2008 Educational Communication and Technology (ECT) Foundation Robert deKeiffer International Fellowship Award. Dr. Botturi, an Italian originally from the suburbs of Milano, works at the Universita della Svizzera italiana (USI, also called the University of Lugano) in its New…

  8. 21 CFR Appendix B to Subpart A of... - List of Authorities

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Control Farmacéutico. For medicinal products for veterinary use: Ministerio de Agricultura, Pesca y...: Agence du Médicament. For veterinary medicinal products: Agence Nationale du Médicament Vétérinaire..., Dipartimento Farmaci e Farmacovigilanza. For medicinal products for veterinary use: Ministero della...

  9. 21 CFR Appendix B to Subpart A of... - List of Authorities

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Control Farmacéutico. For medicinal products for veterinary use: Ministerio de Agricultura, Pesca y...: Agence du Médicament. For veterinary medicinal products: Agence Nationale du Médicament Vétérinaire..., Dipartimento Farmaci e Farmacovigilanza. For medicinal products for veterinary use: Ministero della...

  10. 21 CFR Appendix B to Subpart A of... - List of Authorities

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Control Farmacéutico. For medicinal products for veterinary use: Ministerio de Agricultura, Pesca y...: Agence du Médicament. For veterinary medicinal products: Agence Nationale du Médicament Vétérinaire..., Dipartimento Farmaci e Farmacovigilanza. For medicinal products for veterinary use: Ministero della...

  11. Castelli, Benedetto (1578-1643)

    NASA Astrophysics Data System (ADS)

    Murdin, P.

    2000-11-01

    Mathematician, born in Brescia, Italy, Benedictine monk, professor at Padua. GALILEO's closest scientific collaborator, he defended, and edited Galileo, he helped his sunspot research, inventing the method of projection so as to view safely the Sun's image with a telescope. His book on hydraulics, Della Misura dell'Acque Correnti, or On the Measurement of Running Waters, founded modern hydrodynam...

  12. UBIQUITIN BECOMES UBIQUITOUS IN GA SIGNALING

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research in a wide range of plant species has converged on a story where the hormone gibberellin (GA) stimulates critical stages in plant growth and development by triggering the destruction of negative regulatory DELLA proteins. GA stimulates seed germination, stem elongation, transition to flower...

  13. Fruit development: new directions for an old pathway.

    PubMed

    Moran, Colin N; Halliday, Karen J

    2010-12-21

    A recent study investigating the molecular mechanisms of seed pod shattering has shown that the basic helix-loop-helix (bHLH) proteins INDEHISCENT and ALCATRAZ appear to regulate fruit patterning through gibberellic acid (GA)-DELLA signalling, revealing a central role for bHLH family members in GA response specificity. PMID:21172630

  14. The roles of the GA receptors GID1a, GID1b, and GID1c in sly1-independent GA signaling

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Gibberellin (GA) hormone signaling occurs through proteolytic and non-proteolytic signaling mechanisms when the GA receptor GID1 (GA-INSENSITIVE DWARF 1) binds GA. GA binding to GID1 protein causes a conformational change, enabling GID1 to bind negative regulators of GA responses called DELLA prote...

  15. Interdisciplinary Co-operation (Part II of "Language Learning: Individual Needs, Interdisciplinary Co-operation, Bi- and Multilingualism").

    ERIC Educational Resources Information Center

    1978

    The following papers on interdisciplinary cooperation in second language instruction are included: (1) "Language Teaching: Possibilities for Interdisciplinary Co-operation," by James E. Alatis; (2) "L'insegnamento della letteratura italiana (The Teaching of Italian Literature)," by Ezio Raimondi; (3) "Objective Evaluation and Transparency," by…

  16. An Imaginative Approach to Teaching Writing

    ERIC Educational Resources Information Center

    Cuenca, Carmen Manuel; Carmona, Rodrigo Fernandez

    2012-01-01

    Mindful of the fact that one of the most important ingredients in learning to write in a foreign language is motivation, the authors have experimented in their classes with a wide range of exercises from a very useful source: Gianni Rodari's "Grammatico della Fantasia: lntroduzione all'arte di inventare storie" (Torino: Piccola Biblioteca Einandi,…

  17. Comprehensive gene expression analysis of rice aleurone cells: probing the existence of an alternative gibberellin receptor.

    PubMed

    Yano, Kenji; Aya, Koichiro; Hirano, Ko; Ordonio, Reynante Lacsamana; Ueguchi-Tanaka, Miyako; Matsuoka, Makoto

    2015-02-01

    Current gibberellin (GA) research indicates that GA must be perceived in plant nuclei by its cognate receptor, GIBBERELLIN INSENSITIVE DWARF1 (GID1). Recognition of GA by GID1 relieves the repression mediated by the DELLA protein, a model known as the GID1-DELLA GA perception system. There have been reports of potential GA-binding proteins in the plasma membrane that perceive GA and induce α-amylase expression in cereal aleurone cells, which is mechanistically different from the GID1-DELLA system. Therefore, we examined the expression of the rice (Oryza sativa) α-amylase genes in rice mutants impaired in the GA receptor (gid1) and the DELLA repressor (slender rice1; slr1) and confirmed their lack of response to GA in gid1 mutants and constitutive expression in slr1 mutants. We also examined the expression of GA-regulated genes by genome-wide microarray and quantitative reverse transcription-polymerase chain reaction analyses and confirmed that all GA-regulated genes are modulated by the GID1-DELLA system. Furthermore, we studied the regulatory network involved in GA signaling by using a set of mutants defective in genes involved in GA perception and gene expression, namely gid1, slr1, gid2 (a GA-related F-box protein mutant), and gamyb (a GA-related trans-acting factor mutant). Almost all GA up-regulated genes were regulated by the four named GA-signaling components. On the other hand, GA down-regulated genes showed different expression patterns with respect to GID2 and GAMYB (e.g. a considerable number of genes are not controlled by GAMYB or GID2 and GAMYB). Based on these observations, we present a comprehensive discussion of the intricate network of GA-regulated genes in rice aleurone cells. PMID:25511432

  18. Comprehensive Gene Expression Analysis of Rice Aleurone Cells: Probing the Existence of an Alternative Gibberellin Receptor1

    PubMed Central

    Yano, Kenji; Aya, Koichiro; Hirano, Ko; Ordonio, Reynante Lacsamana; Ueguchi-Tanaka, Miyako; Matsuoka, Makoto

    2015-01-01

    Current gibberellin (GA) research indicates that GA must be perceived in plant nuclei by its cognate receptor, GIBBERELLIN INSENSITIVE DWARF1 (GID1). Recognition of GA by GID1 relieves the repression mediated by the DELLA protein, a model known as the GID1-DELLA GA perception system. There have been reports of potential GA-binding proteins in the plasma membrane that perceive GA and induce α-amylase expression in cereal aleurone cells, which is mechanistically different from the GID1-DELLA system. Therefore, we examined the expression of the rice (Oryza sativa) α-amylase genes in rice mutants impaired in the GA receptor (gid1) and the DELLA repressor (slender rice1; slr1) and confirmed their lack of response to GA in gid1 mutants and constitutive expression in slr1 mutants. We also examined the expression of GA-regulated genes by genome-wide microarray and quantitative reverse transcription-polymerase chain reaction analyses and confirmed that all GA-regulated genes are modulated by the GID1-DELLA system. Furthermore, we studied the regulatory network involved in GA signaling by using a set of mutants defective in genes involved in GA perception and gene expression, namely gid1, slr1, gid2 (a GA-related F-box protein mutant), and gamyb (a GA-related trans-acting factor mutant). Almost all GA up-regulated genes were regulated by the four named GA-signaling components. On the other hand, GA down-regulated genes showed different expression patterns with respect to GID2 and GAMYB (e.g. a considerable number of genes are not controlled by GAMYB or GID2 and GAMYB). Based on these observations, we present a comprehensive discussion of the intricate network of GA-regulated genes in rice aleurone cells. PMID:25511432

  19. Coordinated regulation of apical hook development by gibberellins and ethylene in etiolated Arabidopsis seedlings

    PubMed Central

    An, Fengying; Zhang, Xing; Zhu, Ziqiang; Ji, Yusi; He, Wenrong; Jiang, Zhiqiang; Li, Mingzhe; Guo, Hongwei

    2012-01-01

    Dark-grown Arabidopsis seedlings develop an apical hook when germinating in soil, which protects the cotyledons and apical meristematic tissues when protruding through the soil. Several hormones are reported to distinctly modulate this process. Previous studies have shown that ethylene and gibberellins (GAs) coordinately regulate the hook development, although the underlying molecular mechanism is largely unknown. Here we showed that GA3 enhanced while paclobutrazol repressed ethylene- and EIN3-overexpression (EIN3ox)-induced hook curvature, and della mutant exhibited exaggerated hook curvature, which required an intact ethylene signaling pathway. Genetic study revealed that GA-enhanced hook development was dependent on HOOKLESS 1 (HLS1), a central regulator mediating the input of the multiple signaling pathways during apical hook development. We further found that GA3 induced (and DELLA proteins repressed) HLS1 expression in an ETHYLENE INSENSITIVE 3/EIN3-LIKE 1 (EIN3/EIL1)-dependent manner, whereby EIN3/EIL1 activated HLS1 transcription by directly binding to its promoter. Additionally, DELLA proteins were found to interact with the DNA-binding domains of EIN3/EIL1 and repress EIN3/EIL1-regulated HLS1 expression. Treatment with naphthylphthalamic acid, a polar auxin transport inhibitor, repressed the constitutively exaggerated hook curvature of EIN3ox line and della mutant, supporting that auxin functions downstream of the ethylene and GA pathways in hook development. Taken together, our results identify EIN3/EIL1 as a new class of DELLA-associated transcription factors and demonstrate that GA promotes apical hook formation in cooperation with ethylene partly by inducing the expression of HLS1 via derepression of EIN3/EIL1 functions. PMID:22349459

  20. The first Italian Superconducting Fault Current Limiter: Results of the field testing experience after one year operation

    NASA Astrophysics Data System (ADS)

    Martini, L.; Bocchi, M.; Ascade, M.; Valzasina, A.; Rossi, V.; Ravetta, C.; Angeli, G.

    2014-05-01

    Ricerca sul Sistema Energetico S.p.A. (RSE) has been gaining a relevant experience in the simulation, design and installation of resistive-type Superconducting Fault Current Limiter (SFCL) devices for more than five years in the framework of a R&D national project funded by the Ricerca di Sistema (RdS). The most recent outcome of this research activity is the installation of a resistive-type BSCCO-based 9 kV / 3.4 MVA SFCL device in a single feeder branch of the Medium Voltage (MV) distribution network managed by A2A Reti Elettriche S.p.A (A2A) in the Milano area. This installation represents the first SFCL successfully installed in Italy. In this paper, we report on the main outcomes after a more than 1-year long steady-state field testing activity. The design of an upgraded device to be installed in the same substation has already been initiated: the new SFCL will allow to protect four different feeders, therefore implying a device upgrade up to 15.6 MVA.

  1. Gibberellin acts positively then negatively to control onset of flower formation in Arabidopsis.

    PubMed

    Yamaguchi, Nobutoshi; Winter, Cara M; Wu, Miin-Feng; Kanno, Yuri; Yamaguchi, Ayako; Seo, Mitsunori; Wagner, Doris

    2014-05-01

    The switch to reproductive development is biphasic in many plants, a feature important for optimal pollination and yield. We show that dual opposite roles of the phytohormone gibberellin underpin this phenomenon in Arabidopsis. Although gibberellin promotes termination of vegetative development, it inhibits flower formation. To overcome this effect, the transcription factor LEAFY induces expression of a gibberellin catabolism gene; consequently, increased LEAFY activity causes reduced gibberellin levels. This allows accumulation of gibberellin-sensitive DELLA proteins. The DELLA proteins are recruited by SQUAMOSA PROMOTER BINDING PROTEIN-LIKE transcription factors to regulatory regions of the floral commitment gene APETALA1 and promote APETALA1 up-regulation and floral fate synergistically with LEAFY. The two opposing functions of gibberellin may facilitate evolutionary and environmental modulation of plant inflorescence architecture. PMID:24812402

  2. [Pietro d'Abano and the astrological and scientific image in Padova during the XIV century: from Giotto to Carraresi].

    PubMed

    Mariani Canova, Giordana

    2008-01-01

    The paper intends to prove the incidence that scientific doctrines, mostly Pietro d'Abano's astrological and medical studies, had on Giotto's painting at the Cappella degli Scrovegni in Padova and his lost astrological cycle in the Sala della Ragione. It is emphasized how in no other painting of his, Giotto displayed as much intellectualism as in the Cappella degli Scrovegni. There we can note the importance of the physical representation of the sky and stars and figures' particular physiognomic characterization referable to Pietro d'Abano's theories presented in his astrological treatises and in his Compilation Phisionomiae. Even the ecceptional botanical realism displayed in the representation of plants can be probably referred to Pietro d'Abano's scientific teaching. An hypotetical reconstruction, according to Ptolomeus' theories and Pietro d'Abano's physiognomic, of Giotto's astrological cycle in the Sala della Ragione is also proposed. PMID:19831240

  3. Gibberellin Signaling in Plants – The Extended Version

    PubMed Central

    Schwechheimer, Claus

    2011-01-01

    The plant hormone gibberellin (GA) controls major aspects of plant growth such as germination, elongation growth, flower development, and flowering time. In recent years, a number of studies have revealed less apparent roles for GA in a surprisingly broad set of developmental as well as cell biological processes. The identification of GA receptor proteins on the one end of the signaling cascade, DELLA proteins as central repressors of the pathway and transcription regulators such as the phytochrome interacting factors and the GATA-type transcription factors GNC and CGA1/GNL on the current other end of the signaling cascade have extended our knowledge about how GA and DELLAs regulate a diverse set of plant responses. PMID:22645560

  4. IL TRAPIANTO ORTOTOPICO DEL FEGATO

    PubMed Central

    STARZL, THOMAS E.

    2010-01-01

    E’ormai noto che esiste la possibilità rivoluzionaria di utilizzare il fegato per il trattamento della stadio terminale delle epatopatie. Nel gennaio 1980 si celebra il decimo anna di sopravvivenza con fegato trapiantato (la più lunga della letteratura) di un paziente da noi trattato. Si tratta di uno dei 12 malati sottoposti a trapianto e seguiti per più di 5 anni. La nota positiva di questa tipo di trattamento è rappresentata dall’eccellente tenore di vita che i pazienti conducono e dalla riabilitazione sociale e professionale. La nota negativa è data, invece, dal fatto che i buoni risultati non vengono raggiunti con regolarità e non possono essere previsti con esattezza. In questa breve rassegna considereremo la esperienza da noi fatta presso l’Università di Denver nel Colorado, mettendo in risalto le cause dell’elevata mortalità precoce e le prospettive future di questa mezzo terapeutico. PMID:21572898

  5. Health promotion and surveillance: the establishment of an IUHPE global working group.

    PubMed

    Campostrini, Stefano; McQueen, David V; Evans, Linnea

    2009-12-01

    Following a series of international meetings on behavioral monitoring and surveillance, in 2007 the Italian Ministry of Health (Ministerio della Salute) and the Institute for Health (Istituto Superiore della Sanità) hosted the 5(th) International Conference on Behavioral Risk Factor Surveillance (BRFS) in Rome. A key focus of the conference was on how current surveillance systems could be applied to the field of health promotion, particularly in building the evidence base for health promotion practice. As a result of these discussions, the World Alliance for Risk Factor Surveillance (WARFS), an IUHPE Global Working Group, was formed to work toward providing knowledge and expertise in surveillance as a tool for advancing health promotion. For those IUHPE members interested in participation, this article provides an overview on the strategic direction of WARFS and the newly formed sub-working groups. PMID:20028670

  6. Gibberellin Acts through Jasmonate to Control the Expression of MYB21, MYB24, and MYB57 to Promote Stamen Filament Growth in Arabidopsis

    PubMed Central

    Xiao, Langtao; Soo, Hui Meng; Cheng, Zhiwei; Xie, Daoxin; Peng, Jinrong

    2009-01-01

    Precise coordination between stamen and pistil development is essential to make a fertile flower. Mutations impairing stamen filament elongation, pollen maturation, or anther dehiscence will cause male sterility. Deficiency in plant hormone gibberellin (GA) causes male sterility due to accumulation of DELLA proteins, and GA triggers DELLA degradation to promote stamen development. Deficiency in plant hormone jasmonate (JA) also causes male sterility. However, little is known about the relationship between GA and JA in controlling stamen development. Here, we show that MYB21, MYB24, and MYB57 are GA-dependent stamen-enriched genes. Loss-of-function of two DELLAs RGA and RGL2 restores the expression of these three MYB genes together with restoration of stamen filament growth in GA-deficient plants. Genetic analysis showed that the myb21-t1 myb24-t1 myb57-t1 triple mutant confers a short stamen phenotype leading to male sterility. Further genetic and molecular studies demonstrate that GA suppresses DELLAs to mobilize the expression of the key JA biosynthesis gene DAD1, and this is consistent with the observation that the JA content in the young flower buds of the GA-deficient quadruple mutant ga1-3 gai-t6 rga-t2 rgl1-1 is much lower than that in the WT. We conclude that GA promotes JA biosynthesis to control the expression of MYB21, MYB24, and MYB57. Therefore, we have established a hierarchical relationship between GA and JA in that modulation of JA pathway by GA is one of the prerequisites for GA to regulate the normal stamen development in Arabidopsis. PMID:19325888

  7. Introduzione al Laboratorio di Fisica

    NASA Astrophysics Data System (ADS)

    Ciullo, Giuseppe

    La Fisica (dal greco τὰ ϕυσικὰ: le cose naturali) si pone l'obiettivo di descrivere e prevedere il comportamento dei fenomeni naturali, nonché degli apparati e degli strumenti, che hanno reso e rendono la nostra vita più comoda ed efficiente. Tale obiettivo viene perseguito mediante un'attenta osservazione dei fenomeni, con una conseguente schematizzazione dell'osservazione, per fornire una conoscenza della realtà oggettiva, affidabile, verificabile e condivisibile.

  8. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis.

    PubMed

    Griffiths, Jayne; Murase, Kohji; Rieu, Ivo; Zentella, Rodolfo; Zhang, Zhong-Lin; Powers, Stephen J; Gong, Fan; Phillips, Andrew L; Hedden, Peter; Sun, Tai-ping; Thomas, Stephen G

    2006-12-01

    We investigated the physiological function of three Arabidopsis thaliana homologs of the gibberellin (GA) receptor GIBBERELLIN-INSENSITIVE DWARF1 (GID1) by determining the developmental consequences of GID1 inactivation in insertion mutants. Although single mutants developed normally, gid1a gid1c and gid1a gid1b displayed reduced stem height and lower male fertility, respectively, indicating some functional specificity. The triple mutant displayed a dwarf phenotype more severe than that of the extreme GA-deficient mutant ga1-3. Flower formation occurred in long days but was delayed, with severe defects in floral organ development. The triple mutant did not respond to applied GA. All three GID1 homologs were expressed in most tissues throughout development but differed in expression level. GA treatment reduced transcript abundance for all three GID1 genes, suggesting feedback regulation. The DELLA protein REPRESSOR OF ga1-3 (RGA) accumulated in the triple mutant, whose phenotype could be partially rescued by loss of RGA function. Yeast two-hybrid and in vitro pull-down assays confirmed that GA enhances the interaction between GID1 and DELLA proteins. In addition, the N-terminal sequence containing the DELLA domain is necessary for GID1 binding. Furthermore, yeast three-hybrid assays showed that the GA-GID1 complex promotes the interaction between RGA and the F-box protein SLY1, a component of the SCF(SLY1) E3 ubiquitin ligase that targets the DELLA protein for degradation. PMID:17194763

  9. Corrosion by oxygen in oil and gas well casings

    SciTech Connect

    Martucci, S.; Dinon, M. )

    1993-10-01

    In September 1987, the Della 1 gas well in the Cooper Basin in South Australia blew out to surface. Originally, it was believed that sulfate-reducing bacteria (SRB) had perforated the casings, but numerous workovers on other wells and subsequent investigations indicated that SRB were not the major problem but that corrosion was simply occurring by an oxygen-reduction cathode process. The paper describes the failures and investigates the mechanism of corrosion.

  10. New mechanistic links between sugar and hormone signalling networks.

    PubMed

    Ljung, Karin; Nemhauser, Jennifer L; Perata, Pierdomenico

    2015-06-01

    Plant growth and development must be coordinated with metabolism, notably with the efficiency of photosynthesis and the uptake of nutrients. This coordination requires local connections between hormonal response and metabolic state, as well as long-distance connections between shoot and root tissues. Recently, several molecular mechanisms have been proposed to explain the integration of sugar signalling with hormone pathways. In this work, DELLA and PIF proteins have emerged as hubs in sugar-hormone cross-regulation networks. PMID:26037392

  11. Low Voltage Low Light Imager and Photodetector

    NASA Technical Reports Server (NTRS)

    Nikzad, Shouleh (Inventor); Martin, Chris (Inventor); Hoenk, Michael E. (Inventor)

    2013-01-01

    Highly efficient, low energy, low light level imagers and photodetectors are provided. In particular, a novel class of Della-Doped Electron Bombarded Array (DDEBA) photodetectors that will reduce the size, mass, power, complexity, and cost of conventional imaging systems while improving performance by using a thinned imager that is capable of detecting low-energy electrons, has high gain, and is of low noise.

  12. Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling.

    PubMed

    Shahnejat-Bushehri, Sara; Tarkowska, Danuse; Sakuraba, Yasuhito; Balazadeh, Salma

    2016-01-01

    Gibberellins (GAs) and brassinosteroids (BRs) are important phytohormones that control plant development and responses to environmental cues by involving DELLA proteins and BRASSINAZOLE-RESISTANT1 (BZR1) respectively as key transcription factors. Here, we reveal a new role for JUNGBRUNNEN1 (JUB1) as a transcriptional regulator of GA/BR signalling in Arabidopsis thaliana. JUB1 directly represses the hormone biosynthesis genes GA3ox1 and DWARF4 (DWF4), leading to reduced levels of GAs and BRs and typical GA/BR deficiency phenotypes exhibiting short hypocotyls, dwarfism, late flowering and male sterility. JUB1 also directly represses PHYTOCHROME INTERACTING FACTOR4 (PIF4), a transcription factor connecting hormonal and environmental stimuli. On the other hand, JUB1 activates the DELLA genes GA INSENSITIVE (GAI) and RGA-LIKE 1 (RGL1). In addition, BZR1 and PIF4 act as direct transcriptional repressors upstream of JUB1, establishing a negative feedback loop. Thus, JUB1 forms the core of a robust regulatory module that triggers DELLA accumulation, thereby restricting cell elongation while concomitantly enhancing stress tolerance. PMID:27249348

  13. Flower opening and closure: an update.

    PubMed

    van Doorn, Wouter G; Kamdee, Chanattika

    2014-11-01

    This review is an update of a 2003 review (Journal of Experimental Botany 54,1801-1812) by the same corresponding author. Many examples of flower opening have been recorded using time-lapse photography, showing its velocity and the required elongation growth. Ethylene regulates flower opening, together with at least gibberellins and auxin. Ethylene and gibberellic acid often promote and inhibit, respectively, the expression of DELLA genes and the stability of DELLA proteins. DELLA results in growth inhibition. Both hormones also inhibited and promoted, respectively, the expression of aquaporin genes required for cell elongation. Arabidopsis miRNA319a mutants exhibited narrow and short petals, whereby miRNA319a indirectly regulates auxin effects. Flower opening in roses was controlled by a NAC transcription factor, acting through miRNA164. The regulatory role of light and temperature, in interaction with the circadian clock, has been further elucidated. The end of the life span in many flowers is determined by floral closure. In some species pollination resulted in earlier closure of turgid flowers, compared with unpollinated flowers. It is hypothesized that this pollination-induced effect is only found in flowers in which closure is regulated by ethylene. PMID:25135521

  14. Light curves and absolute magnitudes of four recent fast LMC novae

    NASA Astrophysics Data System (ADS)

    Hearnshaw, J. B.; Livingston, C. M.; Gilmore, A. C.; Kilmartin, P. M.

    2004-05-01

    The light curves of four recent fast LMC novae (Nova LMC 1988a, 1992, 1995, 2000) have been analysed to obtain the parameter t2, the time for a two magnitude decline below maximum light. Using the calibration of Della Valle & Livio (1995), values of MV at maximum are obtained. The weighted mean distance modulus to the LMC based on these novae is 18.89 ± 0.16. This differs significantly drom the distance modulus adpoted by Della Valle & Livio of 18.50, but only differs at the 1σ-level from Feast's (1999) value of 18.70 ± 0.10. The evidence based on these novae suggests that either: (i) DMLMC = 18.50 is too close for the LMC; or (ii) some novae in the LMC, including these four, are significantly underluminous at maximum light compared with those in M31, by about 0.4 mag. This could be a metallicity effect, given that more metal-rich M31 novae were predominantly used by Della Valle & Livio to obtain their calibration.

  15. Elettrodinamica classica

    NASA Astrophysics Data System (ADS)

    Lechner, Kurt

    Nella scoperta della Relatività Ristretta l'Elettrodinamica, rappresentando una teoria relativistica per eccellenza, ha giocato un ruolo fondamentale. Il principio di relativit` a einsteiniana, che afferma che tutte le leggi della fisica devono avere la stessa forma in tutti i sistemi di riferimento inerziali, è emerso con forza da questa teoria ed è andato consolidandosi sempre di più, man mano che le nostre conoscenze del mondo microscopico sono diventate più complete: tutte le interazioni fondamentali rispettano infatti tale principio. Il modo più semplice ed elegante per implementarlo — difatti l'unico di un'utilità concreta — è rappresentato dal paradigma della covarianza a vista nell'ambito del calcolo tensoriale. Questo paradigma è stato applicato con successo a tutte le teorie di carattere fondamentale, come le teorie che descrivono le quattro interazioni fondamentali e le più speculative teorie di superstringa, e mantiene la sua piena efficacia anche in teoria quantistica. La nostra esposizione dell'Elettrodinamica classica si baserà dunque a ragione su questo paradigma.

  16. Molecular mechanism for the interaction between gibberellin and brassinosteroid signaling pathways in Arabidopsis

    PubMed Central

    Gallego-Bartolomé, Javier; Minguet, Eugenio G.; Grau-Enguix, Federico; Abbas, Mohamad; Locascio, Antonella; Thomas, Stephen G.; Alabadí, David; Blázquez, Miguel A.

    2012-01-01

    Plant development is modulated by the convergence of multiple environmental and endogenous signals, and the mechanisms that allow the integration of different signaling pathways is currently being unveiled. A paradigmatic case is the concurrence of brassinosteroid (BR) and gibberellin (GA) signaling in the control of cell expansion during photomorphogenesis, which is supported by physiological observations in several plants but for which no molecular mechanism has been proposed. In this work, we show that the integration of these two signaling pathways occurs through the physical interaction between the DELLA protein GAI, which is a major negative regulator of the GA pathway, and BRASSINAZOLE RESISTANT1 (BZR1), a transcription factor that broadly regulates gene expression in response to BRs. We provide biochemical evidence, both in vitro and in vivo, indicating that GAI inactivates the transcriptional regulatory activity of BZR1 upon their interaction by inhibiting the ability of BZR1 to bind to target promoters. The physiological relevance of this interaction was confirmed by the observation that the dominant gai-1 allele interferes with BR-regulated gene expression, whereas the bzr1-1D allele displays enhanced resistance to DELLA accumulation during hypocotyl elongation. Because DELLA proteins mediate the response to multiple environmental signals, our results provide an initial molecular framework for the integration with BRs of additional pathways that control plant development. PMID:22847438

  17. A new challenge for meteorological measurements: The "MeteoMet" project - Metrology for meteorology

    NASA Astrophysics Data System (ADS)

    Merlone, A.; Lopardo, G.; Antonsen, I.; Bell, S.; Benyon, R.; Boese, N.; del Campo, D.; Dobre, M.; Drnovšek, J.; Elkatmis, A.; Georgin, E.; Grudniewicz, E.; Heinonen, M.; Holstein-Rathlou, C.; Johansson, J.; Klason, P.; Knorova, R.; Melvad, C.; Merrison, J.; Migała, K.; de Podesta, M.; Saathoff, H.; Smorgon, D.; Sparasci, F.; Strnad, R.; Szmyrka-Grzebyk, A.; Vuillermoz, E.

    2013-09-01

    Climate change and its consequences require immediate actions in order to safeguard the environment and economy in Europe and in the rest of world. Aiming to enhance data reliability and reduce uncertainties in climate observations, a joint research project called "MeteoMet - Metrology for Meteorology" started in October 2011 coordinated by the Italian Istituto Nazionale di Ricerca Metrologica (INRiM). The project is focused on the traceability of measurements involved in climate change: surface and upper air measurements of temperature, pressure, humidity, wind speed and direction, solar irradiance and reciprocal influences between measurands. This project will provide the first definition at the European level of validated climate parameters with associated uncertainty budgets and novel criteria for interpretation of historical data series. The big challenge is the propagation of a metrological measurement perspective to meteorological observations. When such an approach will be adopted the requirement of reliable data and robust datasets over wide scales and long terms could be better met.

  18. Morpho-physiolological and qualitative traits of a bread wheat collection spanning a century of breeding in Italy.

    PubMed

    Laino, Paolo; Limonta, Margherita; Gerna, Davide; Vaccino, Patrizia

    2015-01-01

    Evaluation and characterization are crucial steps in the exploitation of germplasm collections. The Sant'Angelo Lodigiano unit of the Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA) maintains a broad collection of Triticum spp, including more than 4000 genotypes of T. aestivum. Such collection represents a wide source of genetic variability for many agronomic and qualitative traits, extremely useful in modern breeding programs. The collection size, however, makes very difficult its management as a whole. A reduced subset, representing the process of wheat breeding in Italy during the last hundred years, was hence identified for an in-depth characterization. The lines were cropped in two locations over two growing seasons, and analyzed using 16 morpho-agronomic and qualitative descriptors. Most of the analysed characters showed a broad variation throughout the collection, allowing to follow the plant ideotype changes across the breeding progress in Italy during the 20th century. PMID:26379457

  19. Morpho-physiolological and qualitative traits of a bread wheat collection spanning a century of breeding in Italy

    PubMed Central

    Laino, Paolo; Limonta, Margherita; Gerna, Davide

    2015-01-01

    Abstract Evaluation and characterization are crucial steps in the exploitation of germplasm collections. The Sant’Angelo Lodigiano unit of the Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA) maintains a broad collection of Triticum spp, including more than 4000 genotypes of T. aestivum. Such collection represents a wide source of genetic variability for many agronomic and qualitative traits, extremely useful in modern breeding programs. The collection size, however, makes very difficult its management as a whole. A reduced subset, representing the process of wheat breeding in Italy during the last hundred years, was hence identified for an in-depth characterization. The lines were cropped in two locations over two growing seasons, and analyzed using 16 morpho-agronomic and qualitative descriptors. Most of the analysed characters showed a broad variation throughout the collection, allowing to follow the plant ideotype changes across the breeding progress in Italy during the 20th century. PMID:26379457

  20. Genetic Biodiversity of Italian Olives (Olea europaea) Germplasm Analyzed by SSR Markers

    PubMed Central

    Vendramin, Giuseppe Giovanni; Chiappetta, Adriana

    2014-01-01

    The olive is an important fruit species cultivated for oil and table olives in Italy and the Mediterranean basin. The conservation of cultivated plants in ex situ collections is essential for the optimal management and use of their genetic resources. The largest ex situ olive germplasm collection consists of approximately 500 Italian olive varieties and corresponding to 85% of the total Italian olive germplasm is maintained at the Consiglio per la Ricerca e sperimentazione per l'Agricoltura, Centro di Ricerca per l'Olivicoltura e l'Industria Olearia (CRA-OLI), in Italy. In this work, eleven preselected nuclear microsatellite markers were used to assess genetic diversity, population structure, and gene flows with the aim of assembling a core collection. The dendrogram obtained utilizing the unweighted pair group method highlights the presence of homonymy and synonymy in olive tree datasets analyzed in this study. 439 different unique genotype profiles were obtained with this combination of 11 loci nSSR, representing 89.8% of the varieties analyzed. The remaining 10.2% comprises different variety pairs in which both accessions are genetically indistinguishable. Clustering analysis performed using BAPS software detected seven groups in Italian olive germplasm and gene flows were determined among identified clusters. We proposed an Italian core collection of 23 olive varieties capturing all detected alleles at microsatellites. The information collected in this study regarding the CRA-OLI ex situ collection can be used for breeding programs, for germplasm conservation, and for optimizing a strategy for the management of olive gene pools. PMID:24723801

  1. Genetic biodiversity of Italian olives (Olea europaea) germplasm analyzed by SSR markers.

    PubMed

    Muzzalupo, Innocenzo; Vendramin, Giuseppe Giovanni; Chiappetta, Adriana

    2014-01-01

    The olive is an important fruit species cultivated for oil and table olives in Italy and the Mediterranean basin. The conservation of cultivated plants in ex situ collections is essential for the optimal management and use of their genetic resources. The largest ex situ olive germplasm collection consists of approximately 500 Italian olive varieties and corresponding to 85% of the total Italian olive germplasm is maintained at the Consiglio per la Ricerca e sperimentazione per l'Agricoltura, Centro di Ricerca per l'Olivicoltura e l'Industria Olearia (CRA-OLI), in Italy. In this work, eleven preselected nuclear microsatellite markers were used to assess genetic diversity, population structure, and gene flows with the aim of assembling a core collection. The dendrogram obtained utilizing the unweighted pair group method highlights the presence of homonymy and synonymy in olive tree datasets analyzed in this study. 439 different unique genotype profiles were obtained with this combination of 11 loci nSSR, representing 89.8% of the varieties analyzed. The remaining 10.2% comprises different variety pairs in which both accessions are genetically indistinguishable. Clustering analysis performed using BAPS software detected seven groups in Italian olive germplasm and gene flows were determined among identified clusters. We proposed an Italian core collection of 23 olive varieties capturing all detected alleles at microsatellites. The information collected in this study regarding the CRA-OLI ex situ collection can be used for breeding programs, for germplasm conservation, and for optimizing a strategy for the management of olive gene pools. PMID:24723801

  2. Physically-based quantitative analysis of soil erosion induced by heavy rainfall on steep slopes

    NASA Astrophysics Data System (ADS)

    Della Sala, Maria; Cuomo, Sabatino; Novità, Antonio

    2014-05-01

    Heavy rainstorms cause either shallow landslides or soil superficial erosion in steep hillslopes covered by coarse unsaturated soils (Cascini et al., 2013), even over large areas (Cuomo and Della Sala, 2013a). The triggering stage of both phenomena is related to ground infiltration, runoff and overland flow (Cuomo and Della Sala, 2013), which are key processes to be investigated. In addition, the mobilization of solid particles deserves a proper physical-based modeling whether a quantitative estimation of solid particles discharge at the outlet of mountain basin is required. In this work, the approaches for soil superficial erosion analysis are firstly reviewed; then, a relevant case study of two medium-sized mountain basins, affected by flow-like phenomena with huge consequences (Cascini et al., 2009) is presented, which motivates a parametric numerical analysis with a physically-based model carried out for a wide class of soil properties and rainfall scenarios (Cuomo et al., 2013b). The achieved results outline that the peak discharge of water and solid particles driven by overland flow depends on rainfall intensity while volumetric solid concentration within the washout is related to the morphometric features of the whole mountain basin. Furthermore, soil suction is outlined as a key factor for the spatial-temporal evolution of infiltration and runoff in the basin, also affecting the discharge of water and solid particles at the outlet of the basin. Based on these insights, selected cases are analyzed aimed to provide a wide class of possible slope erosion scenarios. It is shown that, provided the same amount of cumulated rainfall, the sequence of high and low intensity rainfall events strongly affects the time-discharge at the outlet of the basin without significant variations of the maximum volumetric solid concentration. References Cascini, L., Cuomo, S., Ferlisi, S., Sorbino, G. (2009). Detection of mechanisms for destructive landslides in Campania region

  3. High blood pressure in older subjects with cognitive impairment.

    PubMed

    Mossello, Enrico; Simoni, David

    2016-01-01

    High blood pressure and cognitive impairment often coexist in old age, but their pathophysiological association is complex. Several longitudinal studies have shown that high blood pressure at midlife is a risk factor for cognitive impairment and dementia, although this association is much less clear in old age. The effect of blood pressure lowering in reducing the risk of dementia is only borderline significant in clinical trials of older subjects, partly due to the insufficient follow-up time. Conversely, dementia onset is associated with a decrease of blood pressure values, probably secondary to neurodegeneration. Prognostic effect of blood pressure values in cognitively impaired older subjects is still unclear, with aggressive blood pressure lowering being potentially harmful in this patients category. Brief cognitive screening, coupled with simple motor assessment, are warranted to identify frail older subjects who need a more cautious approach to antihypertensive treatment. Values obtained with ambulatory blood pressure monitoring seem more useful than clinical ones to predict the outcome of cognitively impaired older subjects. Future studies should identify the most appropriate blood pressure targets in older subjects with cognitive impairment. RiassuntoIpertensione arteriosa e decadimento cognitivo spesso coesistono in età avanzata, sebbene la loro associazione sia complessa dal punto di vista fisiopatologico. Diversi studi longitudinali hanno mostrato che elevati valori pressori in età adulta rappresentano un fattore di rischio per decadimento cognitivo e demenza, sebbene tale associazione sia molto meno chiara in età avanzata. L'effetto della terapia antiipertensiva è risultato ai limiti della significatività statistica nel ridurre il rischio di demenza negli studi di intervento su soggetti anziani, in parte a causa della durata insufficiente del follow-up. D'altra parte, l'insorgenza di demenza è associata con una riduzione dei valori pressori

  4. Calorimetria nucleare in collisioni tra ioni pesanti ad energie intermedie

    NASA Astrophysics Data System (ADS)

    Spezzi, L.

    2002-07-01

    Nel presente lavoro di tesi ci si e' occupati di uno studio sistematico di misure di temperatura ed energia di eccitazione in sistemi nucleari formati in reazioni tra ioni pesanti ad energie intermedie. La temperatura a stata misurata tramite due diversi metodi: rapporto tra le rese di isotopi che differiscono di uno stesso numero di nucleoni ( d/t-3He/4He , 3He/4He -6Li/7Li) - temperatura isotopica -, e rapporto tra le popolazioni degli stati eccitati in frammenti instabili (5Li e 6Li) che decadono in particelle leggere in coincidenza - temperatura di emissione. Le misure in esame sono state effettuate presso i Laboratori Nazionali del Sud per i sistemi 93Nb+93Nb, 93Nb+116,124Sn, 116,120Sn+116,124Sn e 116Sn+93Nb ad energie comprese tra 17 e 40 A MeV. L'apparato di rivelazione era costituito da un odoscopio ad alta granularite (96 telescopi Si (50micron)-Si(300micron)-CsI(Tl) ) e basse soglie di rivelazione, utilizzato sia per identificare risonanze in frammenti eccitati che per misure di rese di isotopi di frammenti con 2<=Z<=6, e dal multirivelatore FIASCO che, grazie alla misura contemporanea di posizione e tempo di volo dei frammenti provenienti dal proiettile (PLF - Projectile Like Fragments) e dal bersaglio (TLF - Target Like Fragment), e' stato utilizzato per misurare direttamente l'energia di eccitazione del sistema in studio. Analisi precedenti, condotte nell'ambito di varie collaborazioni, hanno messo in evidenza la possible esistenza di una transizione di fase liquido-gas del primo ordine nella materia nucleare. In tale contesto, ci siamo occupati di una verifica della curva calorica nucleare, ovvero della correlazione esistente fra temperatura ed energia di eccitazione del sistema, indagando, nel contempo, sulla sua dipendenza dalla massa del sistema che si frammenta. Si e' effettuato, inoltre, un confronto fra i due diversi metodi di misura della temperatura (temperatura isotopica e di emissione): l'andamento plateau-like della curva calorica e' stato

  5. Functional analysis of cotton orthologs of GA signal transduction factors GID1 and SLR1.

    PubMed

    Aleman, Lorenzo; Kitamura, Jun; Abdel-mageed, Haggag; Lee, Joohyun; Sun, Yan; Nakajima, Masatoshi; Ueguchi-Tanaka, Miyako; Matsuoka, Makoto; Allen, Randy D

    2008-09-01

    Gibberellic acid (GA) is both necessary and sufficient to promote fiber elongation in cultured fertilized ovules of the upland cotton variety Coker 312. This is likely due to the temporal and spatial regulation of GA biosynthesis, perception, and subsequent signal transduction that leads to alterations in gene expression and morphology. Our results indicate that the initiation of fiber elongation by the application of GA to cultured ovules corresponds with increased expression of genes that encode xyloglucan endotransglycosylase/hydrolase (XTH) and expansin (EXP) that are involved in promoting cell elongation. To gain a better understanding of the GA signaling components in cotton, that lead to such changes in gene expression, two GA receptor genes (GhGID1a and GhGID1b) and two DELLA protein genes (GhSLR1a and GhSLR1b) that are orthologous to the rice GA receptor (GID1) and the rice DELLA gene (SLR1), respectively, were characterized. Similar to the GA biosynthetic genes, expression of GhGID1a and GhGID1b is under the negative regulation by GA while GA positively regulates GhSLR1a. Recombinant GST-GhGID1s showed GA-binding activity in vitro that was augmented in the presence of GhSLR1a, GhSLR1b, or rice SLR1, indicating complex formation between the receptors and repressor proteins. This was further supported by the GA-dependent interaction of these proteins in yeast cells. Ectopic expression of the GhGID1a in the rice gid1-3 mutant plants rescued the GA-insensitive dwarf phenotype, which demonstrates that it is a functional GA receptor. Furthermore, ectopic expression of GhSLR1b in wild type Arabidopsis led to reduced growth and upregulated expression of DELLA-responsive genes. PMID:18506581

  6. Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE ZIM-DOMAIN1 Expression1[C][W

    PubMed Central

    Li, Chao; He, Xin; Luo, Xiangyin; Xu, Li; Liu, Linlin; Min, Ling; Jin, Li; Zhu, Longfu; Zhang, Xianlong

    2014-01-01

    Plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. Previous research has demonstrated that DELLA proteins physically interact with JASMONATE ZIM-DOMAIN1 (JAZ1) and dynamically regulate the interaction of the gibberellin (GA) and jasmonate (JA) signaling pathways. However, whether and how the JAZ1-DELLA regulatory node is regulated at the transcriptional level in plants under normal growth conditions or during pathogen infection is not known. Here, we demonstrate multiple functions of cotton (Gossypium barbadense) GbWRKY1 in the plant defense response and during development. Although GbWRKY1 expression is induced rapidly by methyl jasmonate and infection by Verticillium dahliae, our results show that GbWRKY1 is a negative regulator of the JA-mediated defense response and plant resistance to the pathogens Botrytis cinerea and V. dahliae. Under normal growth conditions, GbWRKY1-overexpressing lines displayed GA-associated phenotypes, including organ elongation and early flowering, coupled with the down-regulation of the putative targets of DELLA. We show that the GA-related phenotypes of GbWRKY1-overexpressing plants depend on the constitutive expression of Gossypium hirsutum GhJAZ1. We also show that GhJAZ1 can be transactivated by GbWRKY1 through TGAC core sequences, and the adjacent sequences of this binding site are essential for binding specificity and affinity to GbWRKY1, as revealed by dual-luciferase reporter assays and electrophoretic mobility shift assays. In summary, our data suggest that GbWRKY1 is a critical regulator mediating the plant defense-to-development transition during V. dahliae infection by activating JAZ1 expression. PMID:25301887

  7. Molecular Characterization of Rht-1 Dwarfing Genes in Hexaploid Wheat12[C][W][OA

    PubMed Central

    Pearce, Stephen; Saville, Robert; Vaughan, Simon P.; Chandler, Peter M.; Wilhelm, Edward P.; Sparks, Caroline A.; Al-Kaff, Nadia; Korolev, Andrey; Boulton, Margaret I.; Phillips, Andrew L.; Hedden, Peter; Nicholson, Paul; Thomas, Stephen G.

    2011-01-01

    The introduction of the Reduced height (Rht)-B1b and Rht-D1b semidwarfing genes led to impressive increases in wheat (Triticum aestivum) yields during the Green Revolution. The reduction in stem elongation in varieties containing these alleles is caused by a limited response to the phytohormone gibberellin (GA), resulting in improved resistance to stem lodging and yield benefits through an increase in grain number. Rht-B1 and Rht-D1 encode DELLA proteins, which act to repress GA-responsive growth, and their mutant alleles Rht-B1b and Rht-D1b are thought to confer dwarfism by producing more active forms of these growth repressors. While no semidwarfing alleles of Rht-A1 have been identified, we show that this gene is expressed at comparable levels to the other homeologs and represents a potential target for producing novel dwarfing alleles. In this study, we have characterized additional dwarfing mutations in Rht-B1 and Rht-D1. We show that the severe dwarfism conferred by Rht-B1c is caused by an intragenic insertion, which results in an in-frame 90-bp insertion in the transcript and a predicted 30-amino acid insertion within the highly conserved amino-terminal DELLA domain. In contrast, the extreme dwarfism of Rht-D1c is due to overexpression of the semidwarfing Rht-D1b allele, caused by an increase in gene copy number. We show also that the semidwarfing alleles Rht-B1d and Rht-B1e introduce premature stop codons within the amino-terminal coding region. Yeast two-hybrid assays indicate that these newly characterized mutations in Rht-B1 and Rht-D1 confer “GA-insensitive” dwarfism by producing DELLA proteins that do not bind the GA receptor GA INSENSITIVE DWARF1, potentially compromising their targeted degradation. PMID:22013218

  8. A GRAS-like gene of sunflower (Helianthus annuus L.) alters the gibberellin content and axillary meristem outgrowth in transgenic Arabidopsis plants.

    PubMed

    Fambrini, M; Mariotti, L; Parlanti, S; Salvini, M; Pugliesi, C

    2015-11-01

    The GRAS proteins belong to a plant transcriptional regulator family that function in the regulation of plant growth and development. Despite their important roles, in sunflower only one GRAS gene (HaDella1) with the DELLA domain has been reported. Here, we provide a functional characterisation of a GRAS-like gene from Helianthus annuus (Ha-GRASL) lacking the DELLA motif. The Ha-GRASL gene contains an intronless open reading frame of 1,743 bp encoding 580 amino acids. Conserved motifs in the GRAS domain are detected, including VHIID, PFYRE, SAW and two LHR motifs. Within the VHII motif, the P-H-N-D-Q-L residues are entirely maintained. Phylogenetic analysis reveals that Ha-GRASL belongs to the SCARECROW LIKE4/7 (SCL4/7) subfamily of the GRAS consensus tree. Accumulation of Ha-GRASL mRNA at the adaxial boundaries from P6/P7 leaf primordia suggests a role of Ha-GRASL in the initiation of median and basal axillary meristems (AMs) of sunflower. When Ha-GRASL is over-expressed in Arabidopsis wild-type plants, the number of lateral bolts increases differently from untransformed plants. However, Ha-GRASL slightly affects the lateral suppressor (las-4-) mutation. Therefore, we hypothesise that Ha-GRASL and LAS are not functionally equivalent. The over-expression of Ha-GRASL reduces metabolic flow of gibberellins (GAs) in Arabidopsis and this modification could be relevant in AM development. Phylogenetic analysis includes LAS and SCL4/7 in the same major clade, suggesting a more recent separation of these genes with respect to other GRAS members. We propose that some features of their ancestor, as well as AM initiation and outgrowth, are partially retained in both LAS and SCL4/7. PMID:26081041

  9. Endogenous Diterpenes Derived from ent-Kaurene, a Common Gibberellin Precursor, Regulate Protonema Differentiation of the Moss Physcomitrella patens1[W][OA

    PubMed Central

    Hayashi, Ken-ichiro; Horie, Keisuke; Hiwatashi, Yuji; Kawaide, Hiroshi; Yamaguchi, Shinjiro; Hanada, Atsushi; Nakashima, Tamotsu; Nakajima, Masatoshi; Mander, Lewis N.; Yamane, Hisakazu; Hasebe, Mitsuyasu; Nozaki, Hiroshi

    2010-01-01

    Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized from ent-kaurene via ent-kaurenoic acid. GAs are ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 GA receptor/DELLA repressor pathway. The lycopod Selaginella moellendorffii biosynthesizes GA and has functional GID1-DELLA signaling components. In contrast, no GAs or functionally orthologous GID1-DELLA components have been found in the moss Physcomitrella patens. However, P. patens produces ent-kaurene, a common precursor for GAs, and possesses a functional ent-kaurene synthase, PpCPS/KS. To assess the biological role of ent-kaurene in P. patens, we generated a PpCPS/KS disruption mutant that does not accumulate ent-kaurene. Phenotypic analysis demonstrates that the mutant has a defect in the protonemal differentiation of the chloronemata to caulonemata. Gas chromatography-mass spectrometry analysis shows that P. patens produces ent-kaurenoic acid, an ent-kaurene metabolite in the GA biosynthesis pathway. The phenotypic defect of the disruptant was recovered by the application of ent-kaurene or ent-kaurenoic acid, suggesting that ent-kaurenoic acid, or a downstream metabolite, is involved in protonemal differentiation. Treatment with uniconazole, an inhibitor of ent-kaurene oxidase in GA biosynthesis, mimics the protonemal phenotypes of the PpCPS/KS mutant, which were also restored by ent-kaurenoic acid treatment. Interestingly, the GA9 methyl ester, a fern antheridiogen, rescued the protonemal defect of the disruption mutant, while GA3 and GA4, both of which are active GAs in angiosperms, did not. Our results suggest that the moss P. patens utilizes a diterpene metabolite from ent-kaurene as an endogenous developmental regulator and provide insights into the evolution of GA functions in land plants. PMID:20488896

  10. "Miracolo dell'arte" Chi ha inventato il telescopio?

    NASA Astrophysics Data System (ADS)

    Sodi, Francesca

    2006-02-01

    Who did invent the telescope? It seems a simple-answering question but this is untrue. The article recall the fundamental steps of the first phase in the telescope history between 16th and 17th century, from the first experiences with the lenses made by the Italian opticians to the Della Porta's theories, from the vicissitudes of the Dutch spectacle-makers to the spreading of the new instrument through the European squares. At last we meet the work of Galileo Galilei, the first man to use the telescope for scientific purposes, understanding all its potentialities.

  11. Water and its effects when drunk cold. The Physician's view (1576-1751).

    PubMed

    Bonfante, L; Calò, L; D'Angelo, A; Favaro, S; Abaterusso, C; Mennella, G; Normanno, M; Spinello, M; Antonello, A

    1999-01-01

    Starting with Baldassare Pisanelli's book Trattato della natura de' cibi et del bere, published in Venice in 1586, the controversies that have kept physicians busy over the centuries regarding the relative importance of water in human health are traced. These controversies were of considerable importance as the Latin word for water 'aqua' is derived from the phrase 'a qua vinimus' (from whence we come). However, until the studies of Nicolas Lemery, one of the most important pharmacologists of the 18th century, the controversies were debated using more theoretical, philosophical arguments. Lemery's studies shifted the debates from those based on philosophical arguments to more physiologically and scientifically based arguments. PMID:10213816

  12. Asymptotic form of the Kohn-Sham correlation potential

    SciTech Connect

    Joubert, D. P.

    2007-07-15

    The density-functional correlation potential of a finite system is shown to asymptotically approach a nonzero constant along a nodal surface of the energetically highest occupied orbital and zero everywhere else. This nonuniform asymptotic form of the correlation potential exactly cancels the nonuniform asymptotic behavior of the exact exchange potential discussed by Della Sala and Goerling [Phys. Rev. Lett. 89, 33003 (2002)]. The sum of the exchange and correlation potentials therefore asymptotically tends to -1/r everywhere, consistent with the asymptotic form of the Kohn-Sham potential as analyzed by Almbladh and von Barth [Phys. Rev. B 31, 3231 (1985)].

  13. Infraspinatus Strength Assessment and Ultrasound Evaluation of Posterior Capsulotenodesis after Arthroscopic Hill-Sachs Remplissage in Traumatic Anterior Glenohumeral Instability: A Retrospective Controlled Study Protocol

    PubMed Central

    Merolla, Giovanni; Porcellini, Giuseppe

    2014-01-01

    Principal investigator and promoter: Giovanni Merolla Co-investigator: Giuseppe Porcellini Investigation performed at the Biomechanics Laboratory “Marco Simoncelli”, D. Cervesi Hospital, Cattolica - Italy Plan of clinical investigation (PCI): Version 1.0 of June 12, 2012 Approved by AV/IRST Ethical Committee (Comitato Etico Area Vasta Romagna) and authorized by AUSL RN (“Determina AUSL Rimini numero 326 del 29/03/2013: Remplissage U.O Chirurgia della Spalla – Valutazione clinica ed ultrasonografica dell’infraspinato dopo capsulo-tenodesi artroscopica (remplissage) nell’instabilità anteriore traumatica di spalla con lesione di Bankart e difetto omerale di Hill-Sachs”) PMID:24809031

  14. Plant ubiquitin-proteasome pathway and its role in gibberellin signaling

    PubMed Central

    Wang, Feng; Deng, Xing Wang

    2011-01-01

    The ubiquitin-proteasome system (UPS) in plants, like in other eukaryotes, targets numerous intracellular regulators and thus modulates almost every aspect of growth and development. The well-known and best-characterized outcome of ubiquitination is mediating target protein degradation via the 26S proteasome, which represents the major selective protein degradation pathway conserved among eukaryotes. In this review, we will discuss the molecular composition, regulation and function of plant UPS, with a major focus on how DELLA protein degradation acts as a key in gibberellin signal transduction and its implication in the regulation of plant growth. PMID:21788985

  15. Giovanni Alessandro Brambilla (1728-1800) and the Imperial Infantry Regiment #22 ("Lacy"). On occasion of the bicentennial of his death.

    PubMed

    Holubar, K; Fatović-Ferencić, S

    2000-09-15

    Giovanni Alessandro Brambilia (1728-1800) was the Chief Surgeon of the Imperial Austrian Army and the first director of the newly founded medico-surgical academy in Vienna (1785). He died in Padua in late July 1800 (29th?), en route from his estate in Lombardy to Vienna, and the chiostro della magnolia in the Santo displays a memorial plaque commemorating his demise. On account of his merits for the education of (military) surgeons and the development of surgery in Austria, this short article shall serve to briefly recall his life and related facts. PMID:11042908

  16. Left atrium remodeling after acute myocardial infarction (results of the GISSI-3 Echo Substudy).

    PubMed

    Popescu, Bogdan A; Macor, Franco; Antonini-Canterin, Francesco; Giannuzzi, Pantaleo; Temporelli, Pier L; Bosimini, Enzo; Gentile, Francesco; Maggioni, Aldo P; Tavazzi, Luigi; Piazza, Rita; Ascione, Luigi; Stoian, Ioana; Cervesato, Eugenio; Nicolosi, Gian L

    2004-05-01

    To evaluate the existence, timing, and determinants of post-infarction left atrial remodeling, we studied a subgroup of 514 patients from the Third Gruppo Italiano per lo Studio della Sopravvivenza nell'Infarto Miocardico Echo Substudy who underwent 4 serial 2-dimensional echocardiograms up to 6 months after acute myocardial infarction. This study is the first to demonstrate, in a large series of patients, the existence of early and late left atrial remodeling after low-risk acute myocardial infarction and the relation of left atrial remodeling to left ventricular remodeling. PMID:15110211

  17. Roles of Gibberellin Catabolism and Signaling in Growth and Physiological Response to Drought and Short-Day Photoperiods in Populus Trees

    PubMed Central

    Zawaski, Christine; Busov, Victor B.

    2014-01-01

    Survival and productivity of perennial plants in temperate zones are dependent on robust responses to prolonged and seasonal cycles of unfavorable conditions. Here we report whole-genome microarray, expression, physiological, and transgenic evidence in hybrid poplar (Populus tremula × Populus alba) showing that gibberellin (GA) catabolism and repressive signaling mediates shoot growth inhibition and physiological adaptation in response to drought and short-day (SD) induced bud dormancy. Both water deprivation and SDs elicited activation of a suite of poplar GA2ox and DELLA encoding genes. Poplar transgenics with up-regulated GA 2-oxidase (GA2ox) and DELLA domain proteins showed hypersensitive growth inhibition in response to both drought and SDs. In addition, the transgenic plants displayed greater drought resistance as evidenced by increased pigment concentrations (chlorophyll and carotenoid) and reductions in electrolyte leakage (EL). Comparative transcriptome analysis using whole-genome microarray showed that the GA-deficiency and GA-insensitivity, SD-induced dormancy, and drought response in poplar share a common regulon of 684 differentially-expressed genes, which suggest GA metabolism and signaling plays a role in plant physiological adaptations in response to alterations in environmental factors. Our results demonstrate that GA catabolism and repressive signaling represents a major route for control of growth and physiological adaptation in response to immediate or imminent adverse conditions. PMID:24465967

  18. Functional characterization of the apple MhGAI1 gene through ectopic expression and grafting experiments in tomatoes.

    PubMed

    Wang, Shuang-Shuang; Liu, Ze-Zhou; Sun, Chao; Shi, Qing-Hua; Yao, Yu-Xin; You, Chun-Xiang; Hao, Yu-Jin

    2012-02-15

    DELLA proteins are essential components of GA signal transduction. MhGAI1 was isolated from the tea crabapple (Malus hupehensis Redh. var. pingyiensis), and it was found to encode a DELLA protein. Mhgai1 is a GA-insensitive allele that was artificially generated via a bridge-PCR approach. Ectopic expression of Mhgai1 reduced plant stature, decreased spontaneous fruit-set-ratio and enhanced drought-tolerance in transgenic tomatoes. In addition, we examined the long-distance movement of Mhgai1 mRNAs by grafting experiments and SqRT-PCR analysis. It was found that the wild-type scions accumulated Mhgai1 transcripts trafficked from the transgenic rootstocks and therefore exhibited dwarf phenotypes. Furthermore, transgenic tomato plants produced more soluble solids, sugars and organic acids compared to wild-type tomatoes, suggesting an involvement of GA signaling in the regulation of fruit quality. Despite noticeable accumulation in the leaves and stems of WT scions, Mhgai1 transcripts were undetectable in flowers and fruit. Therefore, fruit quality was less influenced by the grafting of WT scions onto transgenic rootstocks than they were by the ectopic expression of Mhgai1 in transgenic rootstocks. Taken together, MhGAI1, which functions as a repressor in the GA signaling pathway, and its GA-insensitive allele, Mhgai1, could turn out to be useful targets for the genetic improvement of dwarfing rootstocks in apples. PMID:22153898

  19. Klamath Mountains Ecoregion: Chapter 13 in Status and trends of land change in the Western United States--1973 to 2000

    USGS Publications Warehouse

    Sleeter, Benjamin M.; Calzia, James P.

    2012-01-01

    The Klamath Mountains Ecoregion covers approximately 47,791 km2 (18,452 mi2) of the Klamath and Siskiyou Mountains of northern California and southern Oregon (fig. 1) (Omernik, 1987; U.S. Environmental Protection Agency, 1997). The ecoregion is flanked by the Coast Range Ecoregion to the west, the Southern and Central California Chaparral and Oak Woodlands Ecoregion to the south, the Cascades and the Eastern Cascades Slopes and Foothills Ecoregions to the east, and the Willamette Valley Ecoregion to the north. The mild Mediterranean climate of the ecoregion is characterized by hot, dry summers and wet winters; the amount of winter moisture varies within the ecoregion, decreasing from west to east. The Klamath–Siskiyou Mountains region is widely recognized as an important biodiversity hotspot (Whittaker, 1960; Kruckeberg, 1984; Wagner, 1997; DellaSala and others, 1999), containing more than 3,500 plant species, more than 200 of which are endemic (Sawyer, 2007). A biological assessment by DellaSala and others (1999) ranked the Klamath–Siskiyou Mountains region as the fifth richest coniferous forest in terms of species diversity. In addition, the International Union for the Conservation of Nature considers the region an area of notable botanical importance (Wagner, 1997). Twenty-nine different species of conifers can be found in the Klamath Mountains Ecoregion (Sawyer, 1996).

  20. Calibrazioni a terra e prestazioni in volo di spettrometri ad immagine nel visibile e nel vicino infrarosso per l'esplorazione planetaria

    NASA Astrophysics Data System (ADS)

    Filacchione, Gianrico

    2006-10-01

    progettuale e realizzativa dei parametri e de lle prestazioni strumentali; in generale infatti non solo sarà necessario mantenere le dimensioni, il peso, i consumi elettrici ed il data-volume entro i requisiti disponibili del satellite ma anche ottimizzare i disegni ottici, meccanici ed elettronici al fine di ottenere le massime prestazioni possibili in funzione della natura del target osservato e dei parametri orbitali della missione. Questa tesi descrive i metodi utilizzati per caratterizzare il funzionamento di spettrometri ad immagine per l’esplorazione planetaria. L’attività svolta ha riguardato sia la misura in laboratorio dei parametri strumentali fondamentali sia l’analisi dei dati in volo su cui verificarne ed eventualmente correggerne la risposta. Il funzionamento degli strumenti e la metodologia di calibrazione vengono descritti nelle due parti principali e nelle appendici di questa tesi per gli esperimenti Cassini-VIMS-V e Rosetta-VIRTIS-M. La prima parte è dedicata all’esperimento VIMS-V sul satellite Cassini, il cui disegno ottico, originariamente proposto da Francis Reininger, verrà successivamente riutilizzato e perfezionato su VIRTIS-M (Reininger et al., 1994; Miller et al., 1996). Il capitolo 1 contiene una descrizione dei parametri ottici, strutturali, termici ed elettronici di VIMS-V mettendone in risalto gli aspetti più innovativi del progetto. Vengono inoltre descritti la collocazione dell’esperimento sul satellite Cassini, il coallineamento con gli altri strumenti di remote sensing, i possibili modi operativi ed il formato dei dati scientifici. Il capitolo 2 riguarda il processo di calibrazione ed analisi dei dati in volo di VIMS-V. La procedura eseguita per calibrare radiometricamente lo strumento utilizzando i dati della superficie della Luna e per stimare il flat-field mediante i dati dell’atmosfera di Venere viene dettagliatamente descritta assieme agli algoritmi utilizzati per la rimozione di diversi effetti strumentali (read

  1. [Valutazione delle guardie di sicurezza privata attraverso la Suicide Probability Scale e la Brief Symptom Inventory].

    PubMed

    Dogan, Bulent; Canturk, Gurol; Canturk, Nergis; Guney, Sevgi; Özcan, Ebru

    2016-01-01

    RIASSUNTO. Scopo. Lo scopo di questo studio è stato quello di investigare l'influenza della probabilità di suicidio, con le sue caratteristiche sociodemografiche, e di procurare i dati per la prevenzione del suicidio tra le guardie di sicurezza privata che lavorano in condizioni di stress, essendo a contatto ininterrottamente con eventi negativi e traumatici di vita durante il loro lavoro. Metodi. Hanno partecipato allo studio 200 guardie di sicurezza privata e 200 persone dell'Università di Ankara. Per raccogliere i dati sono stati utilizzati un questionario riguardante le condizioni sociodemografiche dei partecipanti, la Suicide Probability Scale (SPS) e la Brief Symptom Inventory (BSI). Risultati. Genere, stato civile, stipendio, credenze religiose, vivere una situazione di pericolo di vita, passato di tentativi di suicidio, fumare e non avere una malattia cronica hanno causato statisticamente una differenza significativa sui punteggi di SPS tra il gruppo di guardie di sicurezza privata e quello di controllo. In aggiunta, c'è stata una correlazione positiva statisticamente significativa tra i punteggi totali delle sottoscale di SPS e quelli di BSI. Conclusioni. Allo stesso modo degli agenti di polizia e dei gendarmi, le guardie di sicurezza privata sono ad alto rischio di commettere e tentare il suicidio trovandosi in condizioni stressanti di lavoro e anche soffrendo del trauma secondario. È necessario che essi siano consapevoli della propria tendenza al suicidio e avere controlli psichiatrici regolari. PMID:27183512

  2. Transgenic modification of gai or rg/1 causes dwarfing and alters gibberellins, root growth, and metabolite profiles in Populus

    SciTech Connect

    Tschaplinski, Timothy J; Busov, V.; Meilan, R; Pearce, D; Rood, s; Ma, C; Strauss, S

    2006-01-01

    In Arabidopsis and other plants, gibberellin (GA)-regulated responses are mediated by proteins including GAI, RGA and RGL1-3 that contain a functional DELLA domain. Through transgenic modification, we found that DELLA-less versions of GAI (gai) and RGL1 (rgl1) in a Populus tree have profound, dominant effects on phenotype, producing pleiotropic changes in morphology and metabolic profiles. Shoots were dwarfed, likely via constitutive repression of GA-induced elongation, whereas root growth was promoted two- to threefold in vitro. Applied GA{sub 3} inhibited adventitious root production in wild-type poplar, but gai/rgl1 poplars were unaffected by the inhibition. The concentrations of bioactive GA{sub 1} and GA{sub 4} in leaves of gai- and rgl1-expressing plants increased 12- to 64-fold, while the C{sub 19} precursors of GA{sub 1} (GA{sub 53}, GA{sub 44} and GA{sub 19}) decreased three- to ninefold, consistent with feedback regulation of GA 20-oxidase in the transgenic plants. The transgenic modifications elicited significant metabolic changes. In roots, metabolic profiling suggested increased respiration as a possible mechanism of the increased root growth. In leaves, we found metabolite changes suggesting reduced carbon flux through the lignin biosynthetic pathway and a shift towards allocation of secondary storage and defense metabolites, including various phenols, phenolic glucosides, and phenolic acid conjugates.

  3. Nurses: advocating, leading, caring!

    PubMed

    Ferguson, Stephanie L

    2013-01-01

    This speech was delivered on 27 October at the 2012 Conference and general meeting of the Italian Nurses Association CNAI (Consociazione nazionale delle Associazioni infermiere/i) held in Rome from 25 to 27 October 2012. The theme of the conference was "No Nurses No Future". The "No Nurses No Future" is a national campaign developed by the nurses of the Italian Nurses Association to fight for the rights of the profession to sustain not only the practice of the nurse, strong nursing education, research and regulation, but more importantly to ensure that in the future there will be enough nurses in the healthcare workforce to advocate, lead and care for the citizens of Italy.Italian nurses took advantage of the presence of prof. Ferguson and, before travelling to Rome, the Region Lombardy IPASVI Colleges (Coordinamento dei Collegi IPASVI della regione Lombardia) invited her to talk on the same topic during a jointed Conference with CNAI at Circolo della Stampa of Milan on 23rd October. PMID:24083500

  4. INFEZIONI VIRALI CONGENITE, PERINATALI E NEONATALI VIRAL INFECTIONS OF THE FETUS AND NEWBORN INFANT

    PubMed Central

    Tremolada, Sara; Delbue, Serena; Ferrante, Pasquale

    2009-01-01

    Riassunto Alcuni virus possono essere trasmessi verticalmente da madre a figlio in seguito allo sviluppo, da parte della madre, di un’infezione primaria, ricorrente o cronica. La trasmissione materno-fetale dei virus, che può avvenire in utero (infezione congenita), durante il travaglio del parto (infezione perinatale), oppure attraverso l’allattamento (infezione postnatale), può causare aborto spontaneo, morte fetale, ritardo di crescita intrauterino, anomalie congenite e patologie neonatali o postnatali di diversa entità. Alcuni fattori di rischio sembrano influenzare l’incidenza di trasmissione materno-fetale dei virus, come ad esempio la presenza di altre infezioni virali, la carica virale materna, il tipo di infezione (primaria o ricorrente), la durata della rottura delle membrane, la modalità con cui avviene il parto, le condizioni socio-economiche e l’allattamento. Oggi è possibile prevenire la trasmissione materno-fetale di molti virus grazie all’utilizzo di vaccini, immunizzazione passiva e farmaci antivirali. Il rischio di trasmissione delle infezioni perinatali e postnatali, inoltre, può essere diminuito evitando l’allattamento o ricorrendo ad un parto cesareo. PMID:19216201

  5. Comparison of signaling interactions determining annual and perennial plant growth in response to low temperature

    PubMed Central

    Wingler, Astrid

    2015-01-01

    Low temperature inhibits plant growth despite the fact that considerable rates of photosynthetic activity can be maintained. Instead of lower rates of photosynthesis, active inhibition of cell division and expansion is primarily responsible for reduced growth. This results in sink limitation and enables plants to accumulate carbohydrates that act as compatible solutes or are stored throughout the winter to enable re-growth in spring. Regulation of growth in response to temperature therefore requires coordination with carbon metabolism, e.g., via the signaling metabolite trehalose-6-phosphate. The phytohormones gibberellin (GA) and jasmonate (JA) play an important role in regulating growth in response to temperature. Growth restriction at low temperature is mainly mediated by DELLA proteins, whose degradation is promoted by GA. For annual plants, it has been shown that the GA/DELLA pathway interacts with JA signaling and C-repeat binding factor dependent cold acclimation, but these interactions have not been explored in detail for perennials. Growth regulation in response to seasonal factors is, however, particularly important in perennials, especially at high latitudes. In autumn, growth cessation in trees is caused by shortening of the daylength in interaction with phytohormone signaling. In perennial grasses seasonal differences in the sensitivity to GA may enable enhanced growth in spring. This review provides an overview of the signaling interactions that determine plant growth at low temperature and highlights gaps in our knowledge, especially concerning the seasonality of signaling responses in perennial plants. PMID:25628637

  6. Comment on ``Asymptotic form of the Kohn-Sham correlation potential''

    NASA Astrophysics Data System (ADS)

    Holas, A.

    2008-02-01

    For finite systems that have the energetically highest-occupied molecular orbital (HOMO) with an asymptotic nodal surface, Joubert demonstrated recently [Phys. Rev. A 76, 012501 (2007)] strongly anisotropic behavior (in the asymptotic large- r region) of the exact correlation potential of density-functional theory. As is shown by us, this result is a direct and simple consequence of the strong anisotropy of the exact exchange potential obtained by Della Sala and Görling [Phys. Rev. Lett. 89, 033003 (2002); Della Sala and GörlingJ. Chem. Phys. 116, 5374 (2002)] and the assumption about the asymptotic isotropy of the Kohn-Sham (KS) potential (based on the investigation of Almbladh and von Barth [Phys. Rev. B 31, 3231 (1985)] for atoms). Joubert’s result remains a hypothesis only, because the last assumption is in contradiction with the asymptotic strong anisotropy of the KS potential for systems with asymptotic nodal surface of the HOMO, demonstrated by Wu, Ayers, and Yang [J. Chem. Phys. 119, 2978 (2003)]. The correlation potential in the asymptotic region, stemming from their results, is given.

  7. Thermokarst, mantling and Late Amazonian Epoch periglacial-revisions in the Argyre region, Mars

    NASA Astrophysics Data System (ADS)

    Soare, R. J.; Baoini, D.; Conway, S. J.; Dohm, J. M.; Kargel, J. S.

    2015-10-01

    Thermokarst, mantling and Late Amazonian Epoch periglacial-revisions in the Argyre region, Mars R.J. Soare(1), D. Baioni(2), S.J. Conway (3), J.M. Dohm(4)and J.S. Kargel (5)(1) Geography Department, Dawson College, Montreal, Canada H3Z 1A4 rsoare@dawsoncollege.qc.ca.(2) Dipartimento di Scienze della Terra,della Vita e Ambiente, Università di Urbino "Carlo Bo", Campus SOGESTA, 61029 Urbino (PU) Italy. (3) Department of Physical Sciences, Open University, Milton Keynes, United Kingdom, MK7 6AA. (4) The University Museum, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-, Japan.(5) Department of Hydrology & Water Resources, University of Arizona, Tucson, Arizona, USA 85719.1.Introduction Metre to decametre-deep depressions that are rimless, relatively flat-floored, polygonised and scallop-shaped have been widely observed in Utopia Planitia (UP) [e.g. 1-5] and Malea Planum (MP) [6-8]. Although there is some debate about whether the depressions formed by means of sublimation or evaporation, it is commonly believed that the terrain in which the depressions occur is ice-rich.Moreover, most workers assume that this "ice-richness" is derived of a bi-hemispheric, latitudinally-dependent and atmospherically-precipitated mantle that is metres thick [2,4,6-10].

  8. How do the media affect public perception of climate change and geohazards? An Italian case study

    NASA Astrophysics Data System (ADS)

    Pasquaré, Federico A.; Oppizzi, Paolo

    2012-06-01

    This paper uses a combination of a qualitative approach and a quantitative, software-based approach to explore the Italian print media construction of climate change and geohazards between 2007 and 2010. We have broken down our analysis in two sections: the first one deals with the coverage of climate change; the second one focuses on the media representation of hydrogeological hazards and extreme events in Italy. Our software-based, qualitative and quantitative analysis of 1253 storylines from two major Italian broadsheets (the La Repubblica and the Corriere della Sera) has enabled us to assess the presence of typical journalistic frames such as conflict and dramatization, as well as newly-introduced ones such as "prevention vs damages", and "weather vs climate". Our results show that the two newspapers appear to have different "agendas" that might have different impacts on their own readerships: the La Repubblica has been on the forefront of forging a broad public consensus on the need for actions aimed at tackling climate change, whereas the Corriere della Sera has gradually built a journalistic agenda aimed at minimizing the urgency of the climate change problem. As regards the media's representation of hydrogeological hazards, we have confirmed what assessed by previous research, i.e. that Italian journalists still prefer to focus on damages rather than prevention; on a better note, the tendency of the Italian press to confuse weather with climate, blaming climate change for extreme rainfalls causing landslides and floods, has decreased in the last four years.

  9. The LuNa project: experimental didactic modules exploiting portable setups to teach optics in primary and secondary schools

    NASA Astrophysics Data System (ADS)

    Bondani, Maria; Allevi, Alessia; Nardo, Luca; Favale, Fabrizio

    2014-07-01

    The "LuNa" (La natura della Luce nella luce della Natura - The nature of Light in the light of Nature) Project is devoted to the experimental teaching of optics in the different school grades. The basic idea of the Project is that the history of optics and the debate about the nature of light are a meaningful example of how science proceeds in the development of a physical model. Moreover optical phenomena can be presented at different levels of complexity in order to be accessible to students of different age. At the core of the Project are several portable setups that support experimental and partially interactive lectures covering all the aspects of optical phenomena, from geometrical optics to single-photon interference passing through atmospheric optics, spectroscopy, holography and theory of perception. When possible, the setups are realized with simple and easy to find materials so as to be reproducible by teachers and students. Of course, for the most complicated setups (interferometers and holography) research materials are used. Each module is calibrated to fit teachers' requirements either to be included in the curricula of their classes or to be used as an expansion of the optics program.

  10. Round-robin pretest analyses of a 1:6-scale reinforced concrete containment model subject to static internal pressurization

    SciTech Connect

    Clauss, D.B.

    1987-05-01

    Analyses of a 1:6-scale reinforced concrete containment model that will be tested to failure at Sandia National Laboratories in the spring of 1987 were conducted by the following organizations in the United States and Europe: Sandia National Laboratories (USA), Argonne National Laboratory (USA), Electric Power Research Institute (USA), Commissariat a L'Energie Atomique (France), HM Nuclear Installations Inspectorate (UK), Comitato Nazionale per la ricerca e per lo sviluppo dell'Energia Nucleare e delle Energie Alternative (Italy), UK Atomic Energy Authority, Safety and Reliability Directorate (UK), Gesellschaft fuer Reaktorsicherheit (FRG), Brookhaven National Laboratory (USA), and Central Electricity Generating Board (UK). Each organization was supplied with a standard information package, which included construction drawings and actual material properties for most of the materials used in the model. Each organization worked independently using their own analytical methods. This report includes descriptions of the various analytical approaches and pretest predictions submitted by each organization. Significant milestones that occur with increasing pressure, such as damage to the concrete (cracking and crushing) and yielding of the steel components, and the failure pressure (capacity) and failure mechanism are described. Analytical predictions for pressure histories of strain in the liner and rebar and displacements are compared at locations where experimental results will be available after the test. Thus, these predictions can be compared to one another and to experimental results after the test.

  11. Multicentre study of allergic contact cheilitis from toothpastes.

    PubMed

    Francalanci, S; Sertoli, A; Giorgini, S; Pigatto, P; Santucci, B; Valsecchi, R

    2000-10-01

    The present work reports the results of a multicentre study of toothpaste allergic contact cheilitis (TACC) conducted by GIRDCA (Gruppo Italiano Ricerca Dermatiti da Contatto e Ambientali). The study examined 54 patients with eczematous lesions on the lips, the possible cause of which was suspected to be the use of toothpastes. Patch tests were conducted with a standard series, a specially-targeted series (toothpaste cheilitis series, TCS), and with suspected toothpaste(s). A stop-restart test (SRT) was carried out with these, together with a use test to identify possible alternative products. The TCS produced 17 positive reactions in 13 patients, the most frequent being to spearmint oil. Of the 54 patients, 5 displayed positive reactions only to the TCS. The patch tests with toothpaste produced positive reactions in 11/32 patients, the SRT a positive response in 10/12 cases. The diagnosis of TACC was confirmed in 15/54 patients. Alternative products were identified for 5 patients. In conclusion, the allergens most frequently responsible for TACC were the flavourings, and the additional series proved to be useful in many cases (together with patch tests with toothpastes and the SRT) for correct diagnosis and to initiate effective prevention. PMID:11011921

  12. Development, Testing and Installation of a Superconducting Fault Current Limiter for Medium Voltage Distribution Networks

    NASA Astrophysics Data System (ADS)

    Martini, Luciano; Bocchi, Marco; Ascade, Massimo; Valzasina, Angelo; Rossi, Valerio; Angeli, Giuliano; Ravetta, Cesare

    Since 2009 Ricerca sul Sistema Energetico (RSE S.p.A.) has been involved in the design of resistive-type Superconducting Fault Current Limiter (SFCL) for MV applications to be installed in the A2A Reti Elettriche S.p.A distribution grid in the Milano area. The project started with simulations, design and testing activities for a singlephase device; in this paper we report on the successive step, which is concerned with developing, testing and installation at the hosting utility of the final three-phase SFCL prototype. The result of this research activity is a resistive-type 9 kV/3.4 MVA SFCL device, based on first generation (1G) BSCCO tapes, developed by RSE in the framework of a R&D national project. Owing to the positive test results of partial discharge, dielectric and shortcircuit results the three-phase SFCL device is being to be installed in the A2A distribution grid in the Milano area and it is going to be soon energized starting a one-year long field-testing activity.

  13. Final report on EURAMET.M.M-S4: Bilateral comparison of mass standards between INRIM and MCCAA

    NASA Astrophysics Data System (ADS)

    Malengo, Andrea; Testa, Nicola; Torchio, Davide

    2013-01-01

    This report describes the results of the bilateral comparison EURAMET.M.M-S4 (EURAMET 1232) between the Istituto Nazionale di Ricerca Metrologica (INRIM, Italy) and the Malta Competition and Consumer Affairs Authority (MCCAA, Malta). The results of the comparison will be used to support the Calibration and Measurement Capability (CMC) declaration of MCCAA in the field of mass standards calibration. INRIM was the pilot laboratory for this bilateral comparison. For this comparison the travelling standards were nine mass standards having the following nominal values: 50 kg, 5 kg, 1 kg, 200 g, 50 g, 5 g, 500 mg, 100 mg and 2 mg. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by EURAMET, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  14. Measuring Humidity in Methane and Natural Gas with a Microwave Technique

    NASA Astrophysics Data System (ADS)

    Gavioso, R. M.; Madonna Ripa, D.; Benyon, R.; Gallegos, J. G.; Perez-Sanz, F.; Corbellini, S.; Avila, S.; Benito, A. M.

    2014-04-01

    The results of microwave measurements with a quasi-spherical resonator in humid methane samples realized under laboratory conditions at the Istituto Nazionale di Ricerca Metrologica (INRiM) and under industrial conditions in a natural gas sample made available at the facilities of the Technical Manager of the Spanish Gas System and main supplier of natural gas in Spain (ENAGAS) are reported. Measurements at INRiM included vapor phase and condensation tests on methane samples prepared with amount fractions of water between 600 ppm and 5000 ppm at temperatures between 273 K and 295 K and pressures between 150 kPa and 1 MPa. ENAGAS measurements were performed at ambient temperature, 750 kPa on natural gas sampled from the pipeline and successively humidified at amount fractions of water between 140 ppm and 250 ppm for completeness of the comparison with several humidity sensors and instrumentation based on different technologies. To enhance the sensitivity of the microwave method at low humidity, an experimental procedure based on the relative comparison of the dielectric permittivity of the humid gas sample before and after being subject to a chemical drying process was conceived and implemented. The uncertainty budget and the final sensitivity of this procedure are discussed.

  15. Lifestyle and eating habits in a business community.

    PubMed Central

    Stefani, L; Francini, L; Petri, C; Mascherini, G; Scacciati, I; Maffulli, N; Galanti, G

    2014-01-01

    PURPOSE The present study verified, using a validated questionnaire, the presence of unhealthy aspects of lifestyle and chronic degenerative conditions in a working community. METHODS: A cohort from a working community in Italy was investigated using of the INRAN (Istituto Nazionale di Ricerca per gli Alimenti e Nutrizione) questionnaire dedicated to the eating habits and Physical Activity Stages of Change. RESULTS: Most of the 93 subjects (56 females and 37 males, aged 42.0±0.7) recruited reported low levels of physical activity (70 subjects). Slightly more than 50% of the subjects undertook physical activity more than once a week, while 13% did it only once. Food intolerances were reported by 7 subjects (8%), with a high consumption of fruits, cereals and dairy products, low consumption of fish and alcohol, and meat consumption in the normal range. There was a high satisfaction in general quality of life. CONCLUSION: Questionnaire investigations play a role to identify the presence of degenerative chronic conditions in working communities. The self-reported perception of quality of life does not necessarily agree with the lifestyle habits found. Awareness of this aspect could be helpful to plan lifestyle interventions and promote healthy living habits. PMID:25147766

  16. BaR-SPOrt: the instrument to be accommodated at Dome C

    NASA Astrophysics Data System (ADS)

    Zannoni, M.; Carretti, E.; Cortiglioni, S.; Macculi, C.; Ramponi, M.; Sbarra, C.; Ventura, G.; Monari, J.; Poloni, M.; Poppi, S.; Natale, V.; Nesti, R.; Baralis, M.; Peverini, O.; Tascone, R.; Virone, G.; Boscaleri, A.; Boella, G.; Sironi, G.; Gervasi, M.; de Bernardis, P.; Masi, S.; de Petris, M.

    The BaR-SPOrt (Balloon-Borne Radiometers for Sky Polarization Observations) experiment, a program of the Agenzia Spaziale Italiana (ASI) co-funded by PNRA (Progetto Nazionale di Ricerca in Antartide) was originally designed as a payload for long duration balloons flights. The changing scenario, both scientific and strategic, has led us to propose it for the starting winter campaign of at the Concordia Base. Here the instrument and the features making it suitable to operate at Dome-C are described. After the initial setup, BaR-SPOrt should not require any kind of routine intervention by a dedicated base staff. The experiment will just need electrical power (less than 2 kW) and a suitable accommodation on the field. It can be fully monitored and controlled, including the data acquisition, through its own telemetry/telecommand link using IRIDIUM modems. Both the receiver and the critical electronics are housed inside a temperature-controlled vacuum chamber, providing the properly stabilized environment. The cold part of the radiometer employs a closed loop mechanical cryo-cooler that provides temperatures <70 ±0.1 K with low power consumption (<200 W).

  17. Iterative atmospheric parameters estimationof the tropical atmospere

    NASA Astrophysics Data System (ADS)

    Longo, F.; Laneve, G.; Castronuovo, M.

    This work describes an iterative algorithm capable of determining the atmospheric parameters (temperature, water vapor and ozone profiles) by using the observations currently collected at the BSC Station (Malindi -Kenya, 40° E, 3° S) of the Centro di Ricerca Progetto San Marco (CRPSM) of the University of Rome "La Sapienza" (Italy), by using the NOAA ATOVS data. The method is based on the usual approach to the problem of retrieving atmospheric characteristics:- a forward model (using the FASCODE atmospheric code);- a numerical method to find a solution (by means of the Least SquareEstimation (LSE), the Extended Kalman Filter (EKF) and a regularizationmethod for computing stabilized solutions to the ill-posed problems). Moreover, the retrieval method for the temperature, water vapor and ozone is discussed in detail; comparisons are also made with available co-located atmospheric informations from AAPP-ICI software and balloon based soundings. The aim of this paper is to evaluate the accuracy of the estimate of the tropical atmosphere parameters using an autonomous ret rieval algorithm, based on a modified LSE technique, introducing a gain computed from Kalman theory.

  18. Argon Triple-Point Device for Calibration of SPRTs

    NASA Astrophysics Data System (ADS)

    Kołodziej, B.; Manuszkiewicz, H.; Szmyrka-Grzebyk, A.; Lipiński, L.; Kowal, A.; Steur, P. P. M.; Pavese, F.

    2015-03-01

    This paper presents an apparatus for the calibration of long-stem platinum resistance thermometers at the argon triple point , designed at the Institute of Low Temperature and Structural Research, Poland (INTiBS). A hermetically sealed cell filled at the Istituto Nazionale di Ricerca Metrologica, Italy with high purity gas (6N) is the main element of this apparatus. The cell is placed in a cryostat fully immersed in liquid nitrogen. A temperature-controlled shield ensures the quasi-adiabatic condition needed for proper realization of the phase transition. A system for correcting the temperature distribution along the thermometer well is also implemented. The cell cooling and argon solidification is carried out by filling the thermometer well with liquid nitrogen. A LabVIEW computer program written at INTiBS automatically controls the triple-point realization process. The duration of a melting plateau in the apparatus lasts for about 24 h. The melting width for between 20 % and 80 % was mK. The reproducibility of the plateau temperature is better than.

  19. Mean sea-level height variations in the Central Mediterranean

    NASA Astrophysics Data System (ADS)

    Zerbini, Susanna; Bruni, Sara; Errico, Maddalena; Petracca, Fernanda; Raicich, Fabio; Santi, Efisio

    2015-04-01

    The Italian tide gauge network has experienced difficulties during last century. However, historical time series, starting from the end of the ninetieth century, are available in the PSMSL data base (Permanent Service for Mean Sea Level, http://www.psmsl.org/data/). Data from the early 1980s can also be obtained from the data base of ISPRA (Istituto Superiore per la Protezione e la Ricerca Ambientale, http://www.mareografico.it). In 1998, the Italian national tide gauge network (Rete Mareografica Nazionale - RMN) was completely restructured; it consists of 36 homogeneously distributed stations providing measurements sampled every 10 minutes. We have analyzed both the historical and the recent time series of a sub set of stations located in the Tyrrhenian area by using the Empirical Orthogonal Functions (EOF) approach. The EOF analysis allows describing one data set as a linear combination of orthogonal components, or modes, that depend on position only, while the linear combination coefficients are functions of time only. Each mode is associated to a percentage of the total variance of the original data set, which accounts for the relative importance of the corresponding mode of variability. The aim of this work is to identify common modes which could possibly be related to wide area crustal deformation and/or to climatic fluctuations, such as the inverted barometer effect.

  20. The Mar Piccolo of Taranto: an interesting marine ecosystem for the environmental problems studies.

    PubMed

    Cardellicchio, Nicola; Annicchiarico, Cristina; Di Leo, Antonella; Giandomenico, Santina; Spada, Lucia

    2016-07-01

    The National Project RITMARE (la Ricerca ITaliana per il MARE-Italian Research for the sea) started from 1 January 2012. It is one of the national research programs funded by the Italian Ministry of University and Research. RITMARE is coordinated by the National Research Council (CNR) and involves an integrated effort of most of the scientific community working on marine and maritime issues. Within the project, different marine study areas of strategic importance for the Mediterranean have been identified: Among these, the coastal area of Taranto (Ionian Sea, Southern Italy) was chosen for its different industry settlements and the relative impact on the marine environment. In particular, the research has been concentrated on the Mar Piccolo of Taranto, a complex marine ecosystem model important in terms of ecological, social, and economic activities for the presence also of extensive mussel farms. The site has been selected also because the Mar Piccolo area is a characteristic "on field" laboratory suitable to investigate release and diffusion mechanisms of contaminants, evaluate chemical-ecological risks towards the marine ecosystem and human health, and suggest and test potential remediation strategies for contaminated sediments. In this context, within the project RITMARE, a task force of researchers has contributed to elaboration a functioning conceptual model with a multidisciplinary approach useful to identify anthropogenic forcings, its impacts, and solutions of environmental remediation. This paper describes in brief some of the environmental issues related to the Mar Piccolo basin. PMID:26111753

  1. The Italian Cloud-based brokering Infrastructure to sustain Interoperability for Operative Hydrology

    NASA Astrophysics Data System (ADS)

    Boldrini, E.; Pecora, S.; Bussettini, M.; Bordini, F.; Nativi, S.

    2015-12-01

    This work presents the informatics platform carried out to implement the National Hydrological Operative Information System of Italy. In particular, the presentation will focus on the governing aspects of the cloud infrastructure and brokering software that make possible to sustain the hydrology data flow between heterogeneous user clients and data providers.The Institute for Environmental Protection and Research, ISPRA (Istituto Superiore per la Protezione e la Ricerca Ambientale) in collaboration with the Regional Agency for Environmental Protection in the Emilia-Romagna region, ARPA-ER (Agenzia Regionale per la Prevenzione e l´Ambiente dell´Emilia-Romagna) and CNR-IIA (National Research Council of Italy) designed and developed an innovative platform for the discovery and access of hydrological data coming from 19 Italian administrative regions and 2 Italian autonomous provinces, in near real time. ISPRA has deployed and governs such a system. The presentation will introduce and discuss the technological barriers for interoperability as well as social and policy ones. The adopted solutions will be described outlining the sustainability challenges and benefits.

  2. Impact of nutrition since early life on cardiovascular prevention

    PubMed Central

    2012-01-01

    The cardiovascular disease represents the leading cause of morbidity and mortality in Western countries and it is related to the atherosclerotic process. Cardiovascular disease risk factors, such as dyslipidemia, hypertension, insulin resistance, obesity, accelerate the atherosclerotic process which begins in childhood and progresses throughout the life span. The cardiovascular disease risk factor detection and management through prevention delays the atherosclerotic progression towards clinical cardiovascular disease. Dietary habits, from prenatal nutrition, breastfeeding, complementary feeding to childhood and adolescence nutrition play a basic role for this topic. The metabolic and neuroendocrine environment of the fetus is fundamental in the body’s “metabolic programming”. Further several studies have demonstrated the beneficial effects of breastfeeding on cardiovascular risk factors reduction. Moreover the introduction of complementary foods represents another important step, with particular regard to protein intake. An adequate distribution between macronutrients (lipids, proteins and carbohydrates) is required for correct growth development from infancy throughout adolescence and for prevention of several cardiovascular disease risk determinants in adulthood. The purpose of this review is to examine the impact of nutrition since early life on disease. La malattia cardiovascolare rappresenta la principale causa di morbilità e mortalità dei paesi occidentali ed è correlata a degenerazione vascolare aterosclerotica. I fattori di rischio cardiovascolari quali dislipidemia, ipertensione, insulino resistenza e obesità accelerano tale processo il cui esordio è noto sin dell’età pediatrica ed evolve nel corso della vita. L’individuazione e la cura dei fattori di rischio cardiovascolari mediante la prevenzione dei fattori causali ritardano la progressione dell’aterosclerosi e l’insorgenza dei sintomi cardiovascolari. La nutrizione svolge un ruolo

  3. "Galileo's Machine": Late Notes on Free Fall, Projectile Motion, and the Force of Percussion (ca. 1638-1639)

    NASA Astrophysics Data System (ADS)

    Salvia, Stefano

    2014-12-01

    My paper focuses upon the problem of determining the nature, establishing the proportionality, and measuring the intensity of the force of percussion of a projected or falling body, as treated in the Sixth Day of Galileo's Discorsi e dimostrazioni matematiche intorno a due nuove scienze (1638). This fragment was written around 1638-1639 as part of two additional Days of the Discorsi, which Galileo never finished and remained unpublished until 1718. Galileo's last works on percussion show a significant step towards a generalization of his own views on uniform and accelerated motion that will later lead to the Newtonian principle of inertia. The experiment with two equal weights hanging on a pulley, performed in Arcetri during the same period, is compared with the Paduan 1604-1608 experience of the "water balance." Both account for more than three decades of inquiries into what Galileo called forza della percossa, which marked the transition from preclassical to classical mechanics.

  4. Neutrons and music: Imaging investigation of ancient wind musical instruments

    NASA Astrophysics Data System (ADS)

    Festa, G.; Tardino, G.; Pontecorvo, L.; Mannes, D. C.; Senesi, R.; Gorini, G.; Andreani, C.

    2014-10-01

    A set of seven musical instruments and two instruments cares from the 'Fondo Antico della Biblioteca del Sacro Convento' in Assisi, Italy, were investigated through neutron and X-ray imaging techniques. Historical and scientific interests around ancient musical instruments motivate an intense research effort for their characterization using non-destructive and non-invasive techniques. X-ray and neutron tomography/radiography were applied to the study of composite material samples containing wood, hide and metals. The study was carried out at the NEUTRA beamline, PSI (Paul Scherrer Institute, Switzerland). Results of the measurements provided new information on the composite and multi-scale structure, such as: the internal structure of the samples, position of added materials like metals, wood fiber displays, deformations, presence of adhesives and their spatial distribution and novel insight about construction methods to guide the instruments' restoration process.

  5. Some New Reflections on Mr. Palomar

    NASA Astrophysics Data System (ADS)

    Bertolami, O.

    2009-08-01

    The character Mr. Palomar, the alter-ego of the Italian author Italo Calvino, appeared for the first time in 1975 on the pages of the ``Il Corriere della Sera'', and then more or less regularly till its debut as a book in 1983. Through illuminating thoughts and reflections based on observations, for instance, of sea waves, Mr. Palomar discovers that they induce a peaceful and inspirational state of mind that prevents coronary and mental illnesses, and also holds the key to capturing the complexity of the world reducing it into its most elementary mechanisms. In this contribution I will survey some of Mr. Palomar's thoughts while he observes the sky and speculate on others that he might have explored if he shared our contemporary knowledge of the cosmos. I will also discuss the thoughts of other authors on how, cosmological thinking affects the human condition.

  6. Anosognosia for Motor Impairment Following Left Brain Damage

    PubMed Central

    Cocchini, Gianna; Beschin, Nicoletta; Cameron, Annette; Fotopoulou, Aikaterini; Sala, Sergio Della

    2009-01-01

    Anosognosia for motor impairment has been linked to lesions of the right hemisphere. However, left hemisphere damaged patients have often been excluded from investigation because of their associated language deficits. In this study we assessed anosognosia for motor disorders in a group of left hemisphere damaged patients using 2 tools that assess the presence of unawareness—a structured interview that is a common method of assessment of anosognosia in clinical settings, and a new tool, the Visual-Analogue Test for Anosognosia for Motor Impairment (VATAm; Della Sala, Cocchini, Beschin, & Cameron, in press). The structured interview relies heavily on language and enquires about general motor ability whereas the VATAm is less dependent on language abilities and enquires about specific motor tasks. Results suggest that the frequency of anosognosia in left brain damaged patients may have been underestimated due to methodological reasons, and that anosognosia for motor impairment can also be associated with lesions of the left hemisphere. PMID:19254095

  7. Aspherical dust dynamics code for GIADA experiment in the coma of 67P/Churyumov-Gerasimenko

    NASA Astrophysics Data System (ADS)

    Ivanovski, Stavro; Zakharov, Vladimir; Della Corte, Vincenzo; Lucarelli, Francesca; Crifo, Jean-Francois; Rotundi, Alessandra; Fulle, Marco

    2014-05-01

    In 2014, the ESA ROSETTA probe is on its way to face its main scientific objectives by encountering and landing on comet 67P/Churyumov- Gerasimenko. One of the in-situ instrument on board ROSETTA is GIADA (Grain Impact Analyzer and Dust Accumulator)[1], which will measure individual dust grain mass, number density and velocity in the immediate vicinity of the cometary nucleus. Based on the state-of-the-art 3D+t dust coma model [3,4] we developed a 3D+t aspherical dust dynamical code Giaspheria (GIADA aspherical dust analyzer) which treats aspherical dust motion to support the scientific objectives of GIADA. We report the latest improvements in Giaspheria[5,6,7] and the distinctions in the dust dynamics of spherical and aspherical grains using gas solutions for a spherical nucleus not yet data-calibrated. We consider motion of homogeneous, isothermal polygonal convex bodies (close to ellipsoid of revolution with different aspect ratios of axes), moving under influence of three forces: aerodynamic , gravitational and torque. We use the gas distribution (density, velocity, temperature) for a spherical nucleus discussed in [4,8]. We estimate the aerodynamic force from expressions for free molecular interactions and postulate the distribution function of ejection velocity and the distribution function of initial orientation on the surface of the nucleus. We show the dust distribution of aspherical grains at three different heliocentric distances (3AU, 2AU and 1.3 AU) by means of GIPSI simulated GIADA measurements during these stages of the mission. As an input for GIPSI simulations we use the dust and velocity distributions prevised by Giaspheria computations. Acknowledgements: This research has been supported by the Italian Space Agency (ASI) (Ref: n. I/032/05/0) [1] Della Corte V. et al, (2014), Journal of Astronomical Instrumentation (in press). [2] Colangeli, L., et al., Space Science Reviews, Volume 128, Numbers 1-4, 803-821, 2007 [3] Zakharov, V.V., Rodionov A

  8. Reservoirs performances under climate variability: a case study

    NASA Astrophysics Data System (ADS)

    Longobardi, A.; Mautone, M.; de Luca, C.

    2014-09-01

    A case study, the Piano della Rocca dam (southern Italy) is discussed here in order to quantify the system performances under climate variability conditions. Different climate scenarios have been stochastically generated according to the tendencies in precipitation and air temperature observed during recent decades for the studied area. Climate variables have then been filtered through an ARMA model to generate, at the monthly scale, time series of reservoir inflow volumes. Controlled release has been computed considering the reservoir is operated following the standard linear operating policy (SLOP) and reservoir performances have been assessed through the calculation of reliability, resilience and vulnerability indices (Hashimoto et al. 1982), comparing current and future scenarios of climate variability. The proposed approach can be suggested as a valuable tool to mitigate the effects of moderate to severe and persistent droughts periods, through the allocation of new water resources or the planning of appropriate operational rules.

  9. Discovery of a Nova in the Virgo Galaxy M100

    NASA Astrophysics Data System (ADS)

    Ferrarese, Laura; Livio, Mario; Freedman, Wendy; Saha, Abhijit; Stetson, Peter B.; Ford, Holland C.; Hill, Robert J.; Madore, Barry F.

    1996-09-01

    We present the V and I light curves of a nova discovered in the disk of the spiral galaxy M100, located in the Virgo Cluster. In spite of the fact that the light curve is not well sampled around maximum light and the reddening to the nova is not accurately known, by adopting the maximum magnitude versus rate of decline relation by Della Valle & Livio we derive a distance modulus to M100 of mu 0 ~31.0 +/- 0.3 mag, fully consistent with the Cepheid distance modulus of 31.04 +/- 0.17 mag found by Ferrarese et al. from the same set of HST/WFPC2 data.

  10. Safeguarding saproxylic fungal biodiversity in Apennine beech forest priority habitats

    NASA Astrophysics Data System (ADS)

    Maggi, Oriana; Lunghini, Dario; Pecoraro, Lorenzo; Sabatini, Francesco Maria; Persiani, Anna Maria

    2015-04-01

    The FAGUS LIFE Project (LIFE11/NAT/IT/135) targets two European priority habitats, i.e. Habitat 9210* Apennine beech forests with Taxus and Ilex, and Habitat 9220* Apennine beech forests with Abies alba, within two National Parks: Cilento, Vallo di Diano and Alburni; Gran Sasso and Monti della Laga. The current limited distribution of the target habitats is also due to the impact of human activities on forest systems, such as harvesting and grazing. The FAGUS project aims at developing and testing management strategies able to integrate the conservation of priority forest habitats (9210* and 9220*) and the sustainable use of forest resources. In order to assess the responses to different management treatments the BACI monitoring design (Before-After, Control-Intervention) has been applied on forest structure and diversity of focus taxa before and after experimental harvesting treatments. Conventional management of Apennine beech forests impacts a wealth of taxonomic groups, such as saproxylic beetles and fungi, which are threatened throughout Europe by the lack of deadwood and of senescing trees, and by the homogeneous structure of managed forests. Deadwood has been denoted as the most important manageable habitat for biodiversity in forests not only for supporting a wide diversity of organisms, but also for playing a prominent role in several ecological processes, creating the basis for the cycling of photosynthetic energy, carbon, and nutrients stored in woody material. Especially fungi can be regarded as key group for understanding and managing biodiversity associated with decaying wood. The before-intervention field sampling was carried out in Autumn 2013 in 33 monitoring plots across the two national Parks. The occurrence at plot level of both Ascomycota and Basidiomycota sporocarps was surveyed. All standing and downed deadwood with a minimum diameter of 10 cm was sampled for sporocarps larger than 1 mm, and information on decay class and fungal morphogroups