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Sample records for figure-eight-shaped null-flux coil

  1. Magnetic damping forces in figure-eight-shaped null-flux coil suspension systems

    SciTech Connect

    He, Jianliang; Coffey, H.

    1997-08-01

    This paper discusses magnetic damping forces in figure-eight-shaped null-flux coil suspension systems, focusing on the Holloman maglev rocket system. The paper also discusses simulating the damping plate, which is attached to the superconducting magnet by two short-circuited loop coils in the guideway. Closed-form formulas for the magnetic damping coefficient as functions of heave-and-sway displacements are derived by using a dynamic circuit model. These formulas are useful for dynamic stability studies.

  2. Computer modeling and experimental verification of figure-eight-shaped null-flux coil suspension system

    SciTech Connect

    He, J.L.; Mulcahey, T.M.; Rote, D.M.; Kelly, T.

    1994-12-01

    This report discusses the computer modeling and experimental verification of the magnetic forces associated with a figure-eight-shaped null-flux coil suspension system. A set of computer codes called COILGDWY, were developed on the basis of the dynamic circuit model and verified by means of a laboratory model. The experimental verification was conducted with a rotating PVC drum, the surface of which held various types of figure-eight-shaped null-flux coils that interacted with a stationary permanent magnet. The transient and dynamic magnetic forces between the stationary magnet and the rotating conducting coils were measured and compared with results obtained from the computer model. Good agreement between the experimental results and computer simulations was obtained. The computer model can also be used to calculate magnetic forces in a large-scale magnetic-levitation system.

  3. Study on figure-eight-shaped coil electrodynamic suspension magnetic levitation systems without cross-connection

    SciTech Connect

    Ribani, P.L.; Urbano, N.

    2000-01-01

    Two figure-eight-shaped coils for electrodynamic suspension (EDS) magnetic levitation (MAGLEV) systems without cross-connection are proposed and analyzed. The guideway coils are positioned under the MAGLEV vehicle; they are parallel to the horizontal plane. The interaction of a magnetic module on the vehicle, composed of three or four superconducting (SC) coils, with a guideway module, comprised of two figure-eight coils, is studied by means of the dynamic circuit theory. The currents in the SC coils are supposed to be constant in time while they move as a rigid body, with a constant velocity. Some results are presented and compared with those for a standard side-wall cross-connected system.

  4. Designing with null flux coils

    SciTech Connect

    Davey, K.R.

    1997-09-01

    Null flux were suggested by Danby and Powell in the late 1960`s as a useful means for realizing induced lift with little drag. As an array of alternating magnets is translated past a set of null flux coils, the currents induced in these coils act to vertically center the magnets on those coils. At present, one Japanese MAGLEV system company and two American-based companies are employing either null flux or flux eliminating coils in their design for high speed magnetically levitated transportation. The principle question addressed in paper is: what is the proper choice of coil length to magnet length in a null flux system? A generic analysis in the time and frequency domain is laid out with the intent of showing the optimal design specification in terms of coil parameters.

  5. Calculation of motion induced eddy current forces in null flux coils

    SciTech Connect

    Davey, K.; Morris, T.; Shaaf, J.; Rote, D.

    1995-11-01

    Time dependent motion induced eddy current forces can be quite difficult to compute. The movement of null flux coils between magnets is approached using a coupled boundary element-circuit approach to compute the forces on the structure. The technique involves treating the magnets as a separate circuit whose current is dictated by the product of the magnet thickness and the working coercivity. The mutual inductance between the windows of the moving null flux coil and the stationary equivalent magnet coil hold the key for predicting lift, guidance, and drag forces on the coil. The rate of change of these inductances with respect to position determines the forces and currents. A steady state approximation to these forces is derived in addition to a numerical simulation when the steady state assumption is invalid. The results compare favorably to laboratory results from a 4 ft. diameter experimental test wheel.

  6. Dual-keel electrodynamic maglev system

    SciTech Connect

    He, J.; Wang, Z.; Rote, D.M.; Coffey, H.T.; Hull, J.R.; Mulcahy, T.M.; Cai, Y.

    1995-12-31

    A propulsion and stabilization system with a plurality of superconducting magnetic devices affixed to the dual-keels of a vehicle, where the superconducting magnetic devices produce a magnetic field when energized. The system also includes a plurality of figure-eight shaped null-flux coils affixed to opposing vertical sides of slots in a guideway. The figure-eight shaped null-flux coils are vertically oriented, laterally cross-connected in parallel, longitudinally connected in series, and continue the length of the vertical slots providing levitation and guidance force. An external power source energizes the figure-eight shaped null-flux coils to create a magnetic traveling wave that interacts with the magnetic field produced by the superconducting magnets to impart motion to the vehicle.

  7. Dual-keel electrodynamic maglev system

    DOEpatents

    He, Jianliang; Wang, Zian; Rote, Donald M.; Coffey, Howard T.; Hull, John R.; Mulcahy, Thomas M.; Cal, Yigang

    1996-01-01

    A propulsion and stabilization system with a plurality of superconducting magnetic devices affixed to the dual-keels of a vehicle, where the superconducting magnetic devices produce a magnetic field when energized. The system also includes a plurality of figure-eight shaped null-flux coils affixed to opposing vertical sides of slots in a guideway. The figure-eight shaped null-flux coils are vertically oriented, laterally cross-connected in parallel, longitudinally connected in series, and continue the length of the vertical slots providing levitation and guidance force. An external power source energizes the figure-eight shaped null-flux coils to create a magnetic traveling wave that interacts with the magnetic field produced by the superconducting magnets to impart motion to the vehicle.

  8. Dual-keel electrodynamic maglev system

    DOEpatents

    He, J.L.; Wang, Z.; Rote, D.M.; Coffey, H.T.; Hull, J.R.; Mulcahy, T.M.; Cal, Y.

    1996-12-24

    A propulsion and stabilization system is disclosed with a plurality of superconducting magnetic devices affixed to the dual-keels of a vehicle, where the superconducting magnetic devices produce a magnetic field when energized. The system also includes a plurality of figure-eight shaped null-flux coils affixed to opposing vertical sides of slots in a guideway. The figure-eight shaped null-flux coils are vertically oriented, laterally cross-connected in parallel, longitudinally connected in series, and continue the length of the vertical slots providing levitation and guidance force. An external power source energizes the figure-eight shaped null-flux coils to create a magnetic traveling wave that interacts with the magnetic field produced by the superconducting magnets to impart motion to the vehicle. 6 figs.

  9. Dynamic Stability of Lateral and Yawing Motions in the Double Null-Flux EDS System

    NASA Astrophysics Data System (ADS)

    Murai, Toshiaki; Yoshioka, Hiroshi; Sugino, Motohiko

    The double null-flux electro-dynamic suspension (EDS) in the superconducting maglev has the coupling lateral and yawing stiffness, which does not coincide with each other, so special attention should be paid to the dynamic stability of lateral and yawing motions. This paper describes their intrinsic dynamic stability by analyzing the lateral and yawing motions of bogie levitated by the double null-flux EDS.

  10. Study of Japanese electrodynamic-suspension maglev systems

    NASA Astrophysics Data System (ADS)

    He, J. L.; Rote, D. M.; Coffey, H. T.

    1994-04-01

    This report presents the results of a study of the Japanese MLU magnetic levitation (maglev) system. The development of the MLU system is reviewed, and the dynamic circuit model then is introduced and applied to the figure-eight-shaped null-flux coil suspension system. Three different types of figure-eight-shaped null-flux suspension systems are discussed in detail: (1) the figure-eight-shaped null-flux coil suspension system without cross-connection; (2) the combined suspension and guidance system; and (3) the combined propulsion, levitation, and guidance system. The electrodynamic suspension maglev systems developed in Japan seem to be very promising and could result in a commercial application in the near future.

  11. Study of Japanese electrodynamic-suspension maglev systems

    SciTech Connect

    He, J.L.; Rote, D.M.; Coffey, H.T.

    1994-04-01

    This report presents the results of a study of the Japanese MLU magnetic-levitation (maglev) system. The development of the MLU system is reviewed, and the dynamic circuit model then is introduced and applied to the figure-eight-shaped null-flux coil suspension system. Three different types of figure-eight-shaped null-flux suspension systems are discussed in detail: (1) the figure-eight-shaped null-flux coil suspension system without cross-connection; (2) the combined suspension and guidance system; and (3) the combined propulsion, levitation, and guidance system. The electrodynamic-suspension maglev systems developed in Japan seem to be very promising and could result in a commercial application in the near future.

  12. Design approaches and parameters for magnetically levitated transport systems. [Null flux suspension (Maglev)

    SciTech Connect

    Danby, G.T.; Powell, J.R.

    1988-01-01

    Mechanically levitated transport system approaches are assessed with regard to thrust power needs, track cost, suspension stability, and safety. The null flux suspension appears as the favored approach, having the least thrust power requirements, highest stability, and lowest amount of track material. Various null flux configurations are described together with their operating parameters. The Linear Synchronous Motor (LSM) propulsion system is also described for propelling the suspended vehicles. Cryogenics and superconductivity aspects are discussed and the effect of high T/sub c/ superconductors evaluated. 13 refs., 16 figs., 2 tabs.

  13. Forces on a magnet moving past figure-eight coils

    SciTech Connect

    Mulcahy, T.H.; He, Jianliang; Rote, D.M.; Rossing, T.D.

    1993-03-01

    For the first time, the lift, drag, and guidance forces acting on a permanent magnet are measured as the magnet passes over different arrays of figure-eight (null-flux) coils. The experimental results are in good agreement with the predictions of dynamic circuit theory, which is used to explain more optimal coil arrays.

  14. Dual-keel electrodynamic Maglev system

    SciTech Connect

    He, Jianliang; Rote, D.M.; Wang, Zian; Coffey, H.T.

    1995-12-31

    This paper introduces a new concept for an electrodynamic-suspension maglev system that has a dual-keel arrangement. Each keel consists of a row of superconducting magnets aboard the vehicle. The keels move in troughs in the guideway that are each lined with pairs of figure-eight-shaped null-flux coils. Each pair of null-flux coils is cross-connected to produce null-flux suspension and guidance force. The cross-connected figure-eight null-flux coils in each trough are also energized by a three-phase power supply to produce propulsive force. Preliminary analysis shows that the new system has many advantages over other EDS systems in terms of system performance and dynamic stability.

  15. Integrated null-flux suspension and multiphase propulsion system for magnetically-levitated vehicles

    DOEpatents

    Rote, Donald M.; He, Jianliang; Johnson, Larry R.

    1994-01-01

    A propulsion and stabilization system comprising a series of FIG. 8 coils mounted vertically on the walls of the guideway to provide suspension, lateral guidance and propulsion of a magnetically levitated vehicle. This system further allows for altering the magnetic field effects by changing the relative position of the loops comprising the FIG. 8 coils either longitudinally and/or vertically with resulting changes in the propulsion, the vertical stability, and the suspension.

  16. Integrated null-flux suspension and multiphase propulsion system for magnetically-levitated vehicles

    DOEpatents

    Rote, D.M.; He, Jianliang; Johnson, L.R.

    1992-01-01

    This report discusses a propulsion and stabilization system comprising a series of figure 8 coils mounted vertically on the walls of the guideway to provide suspension, lateral guidance and propulsion of a magnetically levitated vehicle. This system further allows for altering the magnetic field effects by changing the relative position of the loops comprising the figure 8 coils either longitudinally and/or vertically with resulting changes in the propulsion, the vertical stability, and the suspension.

  17. Integrated null-flux suspension and multiphase propulsion system for magnetically-levitated vehicles

    DOEpatents

    Rote, D.M.; He, J.; Johnson, L.R.

    1994-01-04

    A propulsion and stabilization system are described comprising a series of coils mounted vertically on the walls of the guideway to provide suspension, lateral guidance, and propulsion of a magnetically levitated vehicle. This system further allows for altering the magnetic field effects by changing the relative position of the loops comprising the coils either longitudinally and/or vertically with resulting changes in the propulsion, the vertical stability, and the suspension. 8 figures.

  18. Forces on a magnet moving past figure-eight coils

    SciTech Connect

    Mulcahy, T.M.; He, J.; Rote, D.M. ); Rossing, T.D. . Dept. of Physics)

    1993-11-01

    Considerable attention has been given to the magnetic levitation of vehicles over guideways consisting of periodically-spaced conducting coils. Examples of proposed guideway configurations include arrays of independent coils (''loop track''), interconnected coils (''ladder track''), two layers of coils (double-layer ''null-flux'' track), and figure-eight coils (single-layer ''null-flux'' track). Typically, widely-separated superconducting magnets are mounted in the vehicle. A system that achieves both lift and guidance from vertical figure-eight coils in the guideway sidewalls has been developed in Japan. This system, when well designed, can have a very large lift-to-drag ratio. The authors conducted an experimental and theoretical investigation of the lift, drag, and guidance forces on a permanent magnet moving close to various arrays of figure-eight coils. The measured time-histories of the forces provide a basis for the evaluation of electrodynamic models and codes developed to analyze the magnetic levitation of vehicles using the discrete suspension coils of the null-flux type. Good correlation was found between the experimental data and the predictions of the code COIL GDWY. The authors report some of the results and discuss their application to the design of maglev systems.

  19. Design Optimization for a Maglev System Employing Flux Eliminating Coils

    NASA Technical Reports Server (NTRS)

    Davey, Kent R.

    1996-01-01

    Flux eliminating coils have received no little attention over the past thirty years as an alternative for realizing lift in a MAGLEV system. When the magnets on board the vehicle are displaced from the equilibrium or null flux point of these coils, they induce current in those coils which act to restore the coil to its null flux or centerline position. The question being addressed in this paper is that of how to choose the best coil for a given system. What appears at first glance to be an innocent question is in fact one that is actually quite involved, encompassing both the global economics and physics of the system. The real key in analyzing that question is to derive an optimization index or functional which represents the cost of the system subject to constraints, the primary constraint being that the vehicle lift itself at a certain threshold speed. Outlined in this paper is one scenario for realizing a total system design which uses sequential quadratic programming techniques.

  20. Computation of magnetic suspension of maglev systems using dynamic circuit theory

    SciTech Connect

    He, J.L.; Rote, D.M.; Coffey, H.T.

    1991-01-01

    Dynamic circuit theory is applied to several magnetic suspensions associated with maglev systems. These suspension systems are the loop-shaped coil guideway, the figure-eight-shaped null-flux coil guideway, and the continuous sheet guideway. Mathematical models, which can be used for the development of computer codes, are provided for each of these suspension systems. The differences and similarities of the models in using dynamic circuit theory are discussed in the paper. The paper emphasizes the transient and dynamic analysis and computer simulation of maglev systems. In general, the method discussed here can be applied to many electrodynamic suspension system design concepts. It is also suited for the computation of the performance of maglev propulsion systems. Numerical examples are presented in the paper. 15 refs., 7 figs., 1 tab.

  1. Computation of magnetic suspension of maglev systems using dynamic circuit theory

    NASA Technical Reports Server (NTRS)

    He, J. L.; Rote, D. M.; Coffey, H. T.

    1992-01-01

    Dynamic circuit theory is applied to several magnetic suspensions associated with maglev systems. These suspension systems are the loop-shaped coil guideway, the figure-eight-shaped null-flux coil guideway, and the continuous sheet guideway. Mathematical models, which can be used for the development of computer codes, are provided for each of these suspension systems. The differences and similarities of the models in using dynamic circuit theory are discussed in the paper. The paper emphasizes the transient and dynamic analysis and computer simulation of maglev systems. In general, the method discussed here can be applied to many electrodynamic suspension system design concepts. It is also suited for the computation of the performance of maglev propulsion systems. Numerical examples are presented in the paper.

  2. Applications of the dynamic circuit theory to maglev suspension systems

    SciTech Connect

    He, Jian Liang; Rote, D.M.; Coffey, H.T.

    1993-11-01

    This paper discusses the applications of dynamic circuit theory to electrodynamic suspension EDS systems. In particular, the paper focuses on the loop-shaped coil and the figure-eight-shaped null-flux coil suspension systems. Mathematical models, including very general force expressions that can be used for the development of computer codes, are provided for each of these suspension systems. General applications and advantages of the dynamic circuit model are summarized. The paper emphasizes the transient and dynamic analysis and computer simulation of maglev systems. In general, the method discussed here can be applied to many EDS maglev design concepts. It is also suited for the computation of the performance of maglev propulsion systems. Numerical examples are presented in the paper to demonstrate the capability of the model.

  3. α/β coiled coils

    PubMed Central

    Hartmann, Marcus D; Mendler, Claudia T; Bassler, Jens; Karamichali, Ioanna; Ridderbusch, Oswin; Lupas, Andrei N; Hernandez Alvarez, Birte

    2016-01-01

    Coiled coils are the best-understood protein fold, as their backbone structure can uniquely be described by parametric equations. This level of understanding has allowed their manipulation in unprecedented detail. They do not seem a likely source of surprises, yet we describe here the unexpected formation of a new type of fiber by the simple insertion of two or six residues into the underlying heptad repeat of a parallel, trimeric coiled coil. These insertions strain the supercoil to the breaking point, causing the local formation of short β-strands, which move the path of the chain by 120° around the trimer axis. The result is an α/β coiled coil, which retains only one backbone hydrogen bond per repeat unit from the parent coiled coil. Our results show that a substantially novel backbone structure is possible within the allowed regions of the Ramachandran space with only minor mutations to a known fold. DOI: http://dx.doi.org/10.7554/eLife.11861.001 PMID:26771248

  4. Pulse Coil Tester

    NASA Technical Reports Server (NTRS)

    Simon, Richard A.

    1988-01-01

    Set of relays tested easily and repeatedly. Pulse coil tester causes coil under test to generate transient voltage; waveform indicates condition of coil. Tester accommodates assembly of up to four coils at a time.

  5. Molecular basis of coiled-coil formation.

    PubMed

    Steinmetz, Michel O; Jelesarov, Ilian; Matousek, William M; Honnappa, Srinivas; Jahnke, Wolfgang; Missimer, John H; Frank, Sabine; Alexandrescu, Andrei T; Kammerer, Richard A

    2007-04-24

    Coiled coils have attracted considerable interest as design templates in a wide range of applications. Successful coiled-coil design strategies therefore require a detailed understanding of coiled-coil folding. One common feature shared by coiled coils is the presence of a short autonomous helical folding unit, termed "trigger sequence," that is indispensable for folding. Detailed knowledge of trigger sequences at the molecular level is thus key to a general understanding of coiled-coil formation. Using a multidisciplinary approach, we identify and characterize here the molecular determinants that specify the helical conformation of the monomeric early folding intermediate of the GCN4 coiled coil. We demonstrate that a network of hydrogen-bonding and electrostatic interactions stabilize the trigger-sequence helix. This network is rearranged in the final dimeric coiled-coil structure, and its destabilization significantly slows down GCN4 leucine zipper folding. Our findings provide a general explanation for the molecular mechanism of coiled-coil formation. PMID:17438295

  6. Coil Welding Aid

    NASA Technical Reports Server (NTRS)

    Wiesenbach, W. T.; Clark, M. C.

    1983-01-01

    Positioner holds coil inside cylinder during tack welding. Welding aid spaces turns of coil inside cylinder and applies contact pressure while coil is tack-welded to cylinder. Device facilitates fabrication of heat exchangers and other structures by eliminating hand-positioning and clamping of individual coil turns.

  7. Starfire poloidal coil systems

    SciTech Connect

    Evans, K. Jr.; Kim, S.H.; Turner, L.R.; Wang, S.T.

    1980-01-01

    The poloidal coils for STARFIRE consists of three systems: (1) equilibrium field (EF) coils; (2) ohmic heating (OH) coils; and (3) correction field (CF) coils. The EF coils are superconducting and lie outside the toroidal field (TF) coils. These coils provide the bulk of the equilibrium field necessary to keep the plasma positioned in the vacuum chamber with the desired cross sectional shape and pressure and current distributions. Having these coils outside of the TF coils requires that they have a larger stored energy and larger currents but eases the assembly, maintenance, and reliability of the coils. The STARFIRE OH system is relatively small compared to tokamaks in which the current is entirely ohmically driven. It is designed to provide sufficient flux in the early startup to raise the plasma current to the point (1 to 2 MA) where the rf current drive can take over.

  8. Protective link for superconducting coil

    DOEpatents

    Umans, Stephen D.

    2009-12-08

    A superconducting coil system includes a superconducting coil and a protective link of superconducting material coupled to the superconducting coil. A rotating machine includes first and second coils and a protective link of superconducting material. The second coil is operable to rotate with respect to the first coil. One of the first and second coils is a superconducting coil. The protective link is coupled to the superconducting coil.

  9. Immune responses to coiled coil supramolecular biomaterials

    PubMed Central

    Rudra, Jai S.; Tripathi, Pulak; Hildeman, David A.; Jung, Jangwook P.; Collier, Joel H.

    2010-01-01

    Self-assembly has been increasingly utilized in recent years to create peptide-based biomaterials for 3D cell culture, tissue engineering, and regenerative medicine, but the molecular determinants of these materials' immunogenicity have remained largely unexplored. In this study, a set of molecules that self-assembled through coiled coil oligomerization was designed and synthesized, and immune responses against them were investigated in mice. Experimental groups spanned a range of oligomerization behaviors and included a peptide from the coiled coil region of mouse fibrin that did not form supramolecular structures, an engineered version of this peptide that formed coiled coil bundles, and a peptide-PEG-peptide triblock bioconjugate that formed coiled coil multimers and supramolecular aggregates. In mice, the native peptide and engineered peptide did not produce any detectable antibody response, and none of the materials elicited detectable peptide-specific T cell responses, as evidenced by the absence of IL-2 and interferon-gamma in cultures of peptide-challenged splenocytes or draining lymph node cells. However, specific antibody responses were elevated in mice injected with the multimerizing peptide-PEG-peptide. Minimal changes in secondary structure were observed between the engineered peptide and the triblock peptide-PEG-peptide, making it possible that the triblock's multimerization was responsible for this antibody response. PMID:20708258

  10. Kinking the coiled coil--negatively charged residues at the coiled-coil interface.

    PubMed

    Straussman, Ravid; Ben-Ya'acov, Ami; Woolfson, Derek N; Ravid, Shoshana

    2007-03-01

    The coiled coil is one of the most common protein-structure motifs. It is believed to be adopted by 3-5% of all amino acids in proteins. It comprises two or more alpha-helical chains wrapped around one another. The sequences of most coiled coils are characterized by a seven-residue (heptad) repeat, denoted (abcdefg)(n). Residues at the a and d positions define the helical interface (core) and are usually hydrophobic, though about 20% are polar or charged. We show that parallel coiled-coils have a unique pattern of their negatively charged residues at the core positions: aspartic acid is excluded from these positions while glutamic acid is not. In contrast the antiparallel structures are more permissive in their amino acid usage. We show further, and for the first time, that incorporation of Asp but not Glu into the a positions of a parallel coiled coil creates a flexible hinge and that the maximal hinge angle is being directly related to the number of incorporated mutations. These new computational and experimental observations will be of use in improving protein-structure predictions, and as rules to guide rational design of novel coiled-coil motifs and coiled coil-based materials. PMID:17207815

  11. NCSX Trim Coil Design

    SciTech Connect

    M. Kalish, A. Brooks, J. Rushinski, R. Upcavage

    2009-05-29

    The National Compact Stellarator Experiment (NCSX) was being constructed at the Princeton Plasma Physics Laboratory in partnership with Oak Ridge National Laboratory before work was stopped in 2008. The objective of this experiment was to develop the stellarator concept and evaluate it's potential as a model for future fusion power plants. Stellarator design requires very precisely positioned Modular Coils of complex shape to form 3D plasmas. In the design of NCSX, Trim Coils were required to compensate for both the positioning of the coils during assembly and the fabrication tolerances of the Modular Coils. Use of the Trim Coils allowed for larger tolerances increasing ease of assembly and decreasing overall cost. A set of Trim coils was developed to suppress the toroidal flux in island regions due to misalignment, magnetic materials, and eddy currents. The requirement imposed upon the design forced the toroidal flux in island regions below 10% of the total toroidal flux in the plasma. An analysis was first performed to evaluate candidate Trim Coil configurations iterating both the size, number, and position of the coils. The design was optimized considering both performance and cost while staying within the tight restraints presented by the space limited geometry. The final design of the Trim Coils incorporated a 48 Coil top bottom symmetric set. Fabrication costs were minimized by having only two coil types and using a planar conventional design with off the shelf commercial conductor. The Trim Coil design incorporated supports made from simple structural shapes assembled together in a way which allowed for adjustment as well as accommodation for the tolerance build up on the mating surfaces. This paper will summarize the analysis that led to the optimization of the Trim Coils set, the trim coil mechanical design, thermal and stress analysis, and the design of the supporting Trim Coil structure.

  12. Electromagnetic pump stator coil

    DOEpatents

    Fanning, Alan W.; Dahl, Leslie R.

    1996-01-01

    An electrical stator coil for an electromagnetic pump includes a continuous conductor strip having first and second terminals at opposite ends thereof and an intermediate section disposed therebetween. The strip is configured in first and second coil halves, with the first coil half including a plurality of windings extending from the first terminal to the intermediate section, and the second coil half including a plurality of windings extending from the second terminal to the intermediate section. The first and second coil halves are disposed coaxially, and the first and second terminals are disposed radially inwardly therefrom with the intermediate section being disposed radially outwardly therefrom.

  13. Electromagnetic pump stator coil

    DOEpatents

    Fanning, A.W.; Dahl, L.R.

    1996-06-25

    An electrical stator coil for an electromagnetic pump includes a continuous conductor strip having first and second terminals at opposite ends thereof and an intermediate section disposed therebetween. The strip is configured in first and second coil halves, with the first coil half including a plurality of windings extending from the first terminal to the intermediate section, and the second coil half including a plurality of windings extending from the second terminal to the intermediate section. The first and second coil halves are disposed coaxially, and the first and second terminals are disposed radially inwardly therefrom with the intermediate section being disposed radially outwardly therefrom. 9 figs.

  14. Biplanar Radiofrequency Coil Design

    NASA Astrophysics Data System (ADS)

    Roberts, D. A.; Insko, E. K.; Bolinger, L.; Leigh, J. S.

    A novel geometry for radiofrequency coil design is described. In this geometry, longitudinal wires of the coil lie on two parallel planes. The currents in the wires of one plane run in the direction opposite to those of the other plane. An analytic solution is provided for the field produced by infinite surface currents running in the biplanar geometry. For the case of discrete wires, computer-generated field maps imply that the homogeneity and sensitivity of the biplanar design are superior to those of a saddle coil, but worse than those obtained in an equivalent discrete cosine or birdcage coil design. Optimization of this coil design was performed using computer simulations. The measured B1 map of an optimized, single-tuned biplanar coil compares favorably to that of an equivalent discrete cosine coil, demonstrating excellent homogeneity in the central region of the coil. A 30 × 24 × 40 cm biplanar coil has been coupled to a 1.5 T imaging system. Images of the human abdomen generated with this coil demonstrate a high degree of homogeneity across nearly all of the sensitive region of the coil.

  15. Coil bobbin for stable superconducting coils

    SciTech Connect

    Kashima, T.; Yamanaka, A.; Nishijima, S.; Okada, T.

    1996-12-31

    The coil bobbin for a.c. coils have been prepared with the high strength polyethylene fiber (DF) reinforced plastics (DFRP) or with hybrid composites reinforced by DF and glass fiber (GF). The coils with the bobbin were found to be markedly stable. The DF has a large negative thermal expansion coefficient and hence the circumferential thermal strain of bobbin can be designed by changing the ratio of DF to GF layer thickness (DF/GF). It was found that the thermal expansion coefficient in the circumferential direction of the outer surface changed from negative to positive with increasing DF/GF and became nearly zero at a DF/GF of approximately 5.1 kA rms class a.c. coils having a bobbin with a negative thermal expansion coefficient or small thermal contraction in the circumferential direction were fabricated and were confirmed to show higher quench current than that with a GFRP bobbin.

  16. Superconducting magnetic coil

    DOEpatents

    Aized, Dawood; Schwall, Robert E.

    1999-06-22

    A superconducting magnetic coil includes a plurality of sections positioned axially along the longitudinal axis of the coil, each section being formed of an anisotropic high temperature superconductor material wound about a longitudinal axis of the coil and having an associated critical current value that is dependent on the orientation of the magnetic field of the coil. The cross section of the superconductor, or the type of superconductor material, at sections along the axial and radial axes of the coil are changed to provide an increased critical current at those regions where the magnetic field is oriented more perpendicularly to the conductor plane, to thereby increase the critical current at these regions and to maintain an overall higher critical current of the coil.

  17. Superconducting magnetic coil

    DOEpatents

    Aized, D.; Schwall, R.E.

    1999-06-22

    A superconducting magnetic coil includes a plurality of sections positioned axially along the longitudinal axis of the coil, each section being formed of an anisotropic high temperature superconductor material wound about a longitudinal axis of the coil and having an associated critical current value that is dependent on the orientation of the magnetic field of the coil. The cross section of the superconductor, or the type of superconductor material, at sections along the axial and radial axes of the coil are changed to provide an increased critical current at those regions where the magnetic field is oriented more perpendicularly to the conductor plane, to thereby increase the critical current at these regions and to maintain an overall higher critical current of the coil. 15 figs.

  18. Superconducting magnetic coil

    DOEpatents

    Aized, Dawood; Schwall, Robert E.

    1996-06-11

    A superconducting magnetic coil includes a plurality of sections positioned axially along the longitudinal axis of the coil, each section being formed of an anisotropic high temperature superconductor material wound about a longitudinal axis of the coil and having an associated critical current value that is dependent on the orientation of the magnetic field of the coil. The cross section of the superconductor, or the type of superconductor material, at sections along the axial and radial axes of the coil are changed to provide an increased critical current at those regions where the magnetic field is oriented more perpendicularly to the conductor plane, to thereby increase the critical current at these regions and to maintain an overall higher critical current of the coil.

  19. MINIMARS choke coil design

    SciTech Connect

    Gurol, H.; Parmer, J.E.

    1986-01-01

    The choke coil is one of the most advanced of all the magnets in the MINIMARS tandem mirror reactor. Recent developments have enabled the high-field choke coil to be much more compact and consume less power than past designs. There are three main technology areas that have had the greatest impact on the choke coil design: (1) superfluid helium (He-II) at 1.8 K; (2) Nb/sub 3/Sn superconductor; and (3) high-strength alloys for conductor reinforcement. The purpose of this paper is to discuss the 24-T MINIMARS choke coil configuration. It is a hybrid design consisting of a superconducting (S/C) background coil and a normal (N/C) insert coil.

  20. Coil system for plasmoid thruster

    NASA Technical Reports Server (NTRS)

    Eskridge, Richard H. (Inventor); Lee, Michael H. (Inventor); Martin, Adam K. (Inventor); Fimognari, Peter J. (Inventor)

    2010-01-01

    A coil system for a plasmoid thruster includes a bias coil, a drive coil and field coils. The bias and drive coils are interleaved with one another as they are helically wound about a conical region. A first field coil defines a first passage at one end of the conical region, and is connected in series with the bias coil. A second field coil defines a second passage at an opposing end of the conical region, and is connected in series with the bias coil.

  1. Open coil traction system.

    PubMed

    Vibhute, Pavankumar Janardan

    2012-01-01

    Sliding mechanics have become a popular method for space closure, with the development of preadjusted edgewise appliances. Furthermore, various space closing auxiliaries have been developed and extensively evaluated for their clinical efficiency. Their effectiveness is enhanced with optimum force magnitude and low load deflection rate/force decay. With the advent of nickel-titanium (Ni-Ti) springs in orthodontics, load deflection rates have been markedly reduced. To use Ni-Ti springs, clinicians have to depend upon prefabricated closed coil springs. The open coil traction system, or open coil retraction spring, is developed utilizing Ni-Ti open coil springs for orthodontic space closure. This article describes the fabrication and clinical application of the open coil traction system, which has a number of advantages. It sustains a low load deflection rate with optimum force magnitude, and its design is adjustable for a desired length and force level. It is fail-safe for both activation and deactivation (ie, it cannot be overactivated, and the decompression limit of the open coil is controlled by the operator). The open coil traction system can be offset from the mucosa to help reduce soft tissue impingement. PMID:22567645

  2. Commercial applications for COIL

    NASA Astrophysics Data System (ADS)

    Solomon, Wayne C.; Carroll, David L.; King, D. M.; Fockler, L. A.; Stromberg, D. S.; Sexauer, M.; Milmoe, A.; Sentman, Lee H.

    2000-01-01

    The chemical oxygen-iodine laser (COIL) is a high power, fiber deliverable tool, which can be used for a number of different industrial applications. COIL is of particular interest because of its short fiber deliverable wavelength, high scaleable continuous wave power, and excellent material interaction properties. In past research the University of Illinois at Urbana-Champaign identified and decommissioning and decontamination (DD) of nuclear facilities as a primary focus for COIL technology. DD will be a major challenge in the coming decades. The use of a robotically driven fiber delivered cutting/ablation tool in contaminated areas promises to lower risks to workers for the DD mission. Further, the high cutting speed of COIL will significantly reduce the time required to cut contaminated equipment, reducing costs. The high power of COIL will permit the dismantling of thick stacks of piping and equipment as well as reactor vessels. COIL is very promising for the removal of material from contaminated surfaces, perhaps to depths thicker than an inch. Laser cutting and ablation minimizes dust and fumes, which reduces the required number of high efficiency particulate accumulator filters, thus reducing costly waste disposal. Other potential industrial applications for COIL are shipbuilding, automotive manufacturing, heavy machinery manufacturing, tasks requiring underwater cutting or welding, and there appear to be very promising applications for high powers lasers in the oil industry.

  3. Correction coil cable

    DOEpatents

    Wang, S.T.

    1994-11-01

    A wire cable assembly adapted for the winding of electrical coils is taught. A primary intended use is for use in particle tube assemblies for the Superconducting Super Collider. The correction coil cables have wires collected in wire array with a center rib sandwiched therebetween to form a core assembly. The core assembly is surrounded by an assembly housing having an inner spiral wrap and a counter wound outer spiral wrap. An alternate embodiment of the invention is rolled into a keystoned shape to improve radial alignment of the correction coil cable on a particle tube in a particle tube assembly. 7 figs.

  4. Coil spring venting arrangement

    DOEpatents

    McCugh, R.M.

    1975-10-21

    A simple venting device for trapped gas pockets in hydraulic systems is inserted through a small access passages, operated remotely, and removed completely. The device comprises a small diameter, closely wound coil spring which is pushed through a guide temporarily inserted in the access passage. The guide has a central passageway which directs the coil spring radially upward into the pocket, so that, with the guide properly positioned for depth and properly oriented, the coil spring can be pushed up into the top of the pocket to vent it. By positioning a seal around the free end of the guide, the spring and guide are removed and the passage is sealed.

  5. ELECTRICAL COIL STRUCTURE

    DOEpatents

    Baker, W.R.; Hartwig, A.

    1962-09-25

    A compactly wound electrical coil is designed for carrying intense pulsed currents such as are characteristic of controlled thermonuclear reaction devices. A flat strip of conductor is tightly wound in a spiral with a matching flat strip of insulator. To provide for a high fluid coolant flow through the coil with minimum pumping pressure, a surface of the conductor is scored with parallel transverse grooves which form short longitudinal coolant pasaages when the conductor is wound in the spiral configuration. Owing to this construction, the coil is extremely resistant to thermal and magnetic shock from sudden high currents. (AEC)

  6. Magnetic microhelix coil structures.

    PubMed

    Smith, Elliot J; Makarov, Denys; Sanchez, Samuel; Fomin, Vladimir M; Schmidt, Oliver G

    2011-08-26

    Together with the well-known ferro- and antiferromagnetic ordering, nature has created a variety of complex helical magnetic configurations. Here, we design and investigate three-dimensional microhelix coil structures that are radial-, corkscrew-, and hollow-bar-magnetized. The magnetization configurations of the differently magnetized coils are experimentally revealed by probing their specific dynamic response to an external magnetic field. Helix coils offer an opportunity to realize microscale geometries of the magnetic toroidal moment, observed so far only in bulk multiferroic materials. PMID:21929266

  7. Magnetic Microhelix Coil Structures

    NASA Astrophysics Data System (ADS)

    Smith, Elliot J.; Makarov, Denys; Sanchez, Samuel; Fomin, Vladimir M.; Schmidt, Oliver G.

    2011-08-01

    Together with the well-known ferro- and antiferromagnetic ordering, nature has created a variety of complex helical magnetic configurations. Here, we design and investigate three-dimensional microhelix coil structures that are radial-, corkscrew-, and hollow-bar-magnetized. The magnetization configurations of the differently magnetized coils are experimentally revealed by probing their specific dynamic response to an external magnetic field. Helix coils offer an opportunity to realize microscale geometries of the magnetic toroidal moment, observed so far only in bulk multiferroic materials.

  8. Disruption of Bcr-Abl Coiled Coil Oligomerization by Design*

    PubMed Central

    Dixon, Andrew S.; Pendley, Scott S.; Bruno, Benjamin J.; Woessner, David W.; Shimpi, Adrian A.; Cheatham, Thomas E.; Lim, Carol S.

    2011-01-01

    Oligomerization is an important regulatory mechanism for many proteins, including oncoproteins and other pathogenic proteins. The oncoprotein Bcr-Abl relies on oligomerization via its coiled coil domain for its kinase activity, suggesting that a designed coiled coil domain with enhanced binding to Bcr-Abl and reduced self-oligomerization would be therapeutically useful. Key mutations in the coiled coil domain of Bcr-Abl were identified that reduce homo-oligomerization through intermolecular charge-charge repulsion yet increase interaction with the Bcr-Abl coiled coil through additional salt bridges, resulting in an enhanced ability to disrupt the oligomeric state of Bcr-Abl. The mutations were modeled computationally to optimize the design. Assays performed in vitro confirmed the validity and functionality of the optimal mutations, which were found to exhibit reduced homo-oligomerization and increased binding to the Bcr-Abl coiled coil domain. Introduction of the mutant coiled coil into K562 cells resulted in decreased phosphorylation of Bcr-Abl, reduced cell proliferation, and increased caspase-3/7 activity and DNA segmentation. Importantly, the mutant coiled coil domain was more efficacious than the wild type in all experiments performed. The improved inhibition of Bcr-Abl through oligomeric disruption resulting from this modified coiled coil domain represents a viable alternative to small molecule inhibitors for therapeutic intervention. PMID:21659527

  9. Theory of myelin coiling.

    PubMed

    Huang, J-R

    2006-04-01

    A new model is proposed to explain coiling of myelins composed of fluid bilayers. This model allows the constituent bilayer cylinders of a myelin to be non-coaxial and the bilayer lateral tension to vary from bilayer to bilayer. The calculations show that a myelin would bend or coil to lower its free energy when the bilayer lateral tension is sufficiently large. From a mechanical point of view, the proposed coiling mechanism is analogous to the classical Euler buckling of a thin elastic rod under axial compression. The analysis of a simple two-bilayer case suggests that a bilayer lateral tension of about 1 dyne/cm can easily induce coiling of myelins of typical lipid bilayers. This model signifies the importance of bilayer lateral tension in determining the morphology of myelinic structures. PMID:16465468

  10. Borehole induction coil transmitter

    DOEpatents

    Holladay, Gale; Wilt, Michael J.

    2002-01-01

    A borehole induction coil transmitter which is a part of a cross-borehole electromagnetic field system that is used for underground imaging applications. The transmitter consists of four major parts: 1) a wound ferrite or mu-metal core, 2) an array of tuning capacitors, 3) a current driver circuit board, and 4) a flux monitor. The core is wound with several hundred turns of wire and connected in series with the capacitor array, to produce a tuned coil. This tuned coil uses internal circuitry to generate sinusoidal signals that are transmitted through the earth to a receiver coil in another borehole. The transmitter can operate at frequencies from 1-200 kHz and supplies sufficient power to permit the field system to operate in boreholes separated by up to 400 meters.

  11. NCSX Toroidal Field Coil Design

    SciTech Connect

    Kalish, M.; Rushinski, J.; Myatt, L.; Brooks, A.; Dahlgren, F.; Chrzanowski, J.; Reiersen, W.; Freudenberg, K.

    2005-10-07

    The National Compact Stellarator Experiment (NCSX) is an experimental device whose design and construction is underway at the Department of Energy's Princeton Plasma Physics Laboratory (PPPL). The primary coil systems for the NCSX device consist of the twisted plasma-shaping Modular Coils, the Poloidal Field Coils, and the Toroidal Field (TF) Coils. The TF Coils are D-shaped coils wound from hollow copper conductor, and vacuum impregnated with a glass-epoxy resin system. There are 18 identical, equally spaced TF coils providing 1/R field at the plasma. They operate within a cryostat, and are cooled by LN2, nominally, to 80K. Wedge shaped castings are assembled to the inboard face of these coils, so that inward radial loads are reacted via the nesting of each of the coils against their adjacent partners. This paper outlines the TF Coil design methodology, reviews the analysis results, and summarizes how the design and analysis support the design requirements.

  12. Hierarchical Cascades of Instability Govern the Mechanics of Coiled Coils: Helix Unfolding Precedes Coil Unzipping

    PubMed Central

    Hamed, Elham; Keten, Sinan

    2014-01-01

    Coiled coils are a fundamental emergent motif in proteins found in structural biomaterials, consisting of α-helical secondary structures wrapped in a supercoil. A fundamental question regarding the thermal and mechanical stability of coiled coils in extreme environments is the sequence of events leading to the disassembly of individual oligomers from the universal coiled-coil motifs. To shed light on this phenomenon, here we report atomistic simulations of a trimeric coiled coil in an explicit water solvent and investigate the mechanisms underlying helix unfolding and coil unzipping in the assembly. We employ advanced sampling techniques involving steered molecular dynamics and metadynamics simulations to obtain the free-energy landscapes of single-strand unfolding and unzipping in a three-stranded assembly. Our comparative analysis of the free-energy landscapes of instability pathways shows that coil unzipping is a sequential process involving multiple intermediates. At each intermediate state, one heptad repeat of the coiled coil first unfolds and then unzips due to the loss of contacts with the hydrophobic core. This observation suggests that helix unfolding facilitates the initiation of coiled-coil disassembly, which is confirmed by our 2D metadynamics simulations showing that unzipping of one strand requires less energy in the unfolded state compared with the folded state. Our results explain recent experimental findings and lay the groundwork for studying the hierarchical molecular mechanisms that underpin the thermomechanical stability/instability of coiled coils and similar protein assemblies. PMID:25028889

  13. AAFreqCoil: a new classifier to distinguish parallel dimeric and trimeric coiled coils.

    PubMed

    Wang, Xiaofeng; Zhou, Yuan; Yan, Renxiang

    2015-07-01

    Coiled coils are characteristic rope-like protein structures, constituted by one or more heptad repeats. Native coiled-coil structures play important roles in various biological processes, while the designed ones are widely employed in medicine and industry. To date, two major oligomeric states (i.e. dimeric and trimeric states) of a coiled-coil structure have been observed, plausibly exerting different biological functions. Therefore, exploration of the relationship between heptad repeat sequences and coiled coil structures is highly important. In this paper, we develop a new method named AAFreqCoil to classify parallel dimeric and trimeric coiled coils. Our method demonstrated its competitive performance when benchmarked based on 10-fold cross validation and jackknife cross validation. Meanwhile, the rules that can explicitly explain the prediction results of the test coiled coil can be extracted from the AAFreqCoil model for a better explanation of user predictions. A web server and stand-alone program implementing the AAFreqCoil algorithm are freely available at . PMID:25918905

  14. Correction coil cable

    DOEpatents

    Wang, Sou-Tien

    1994-11-01

    A wire cable assembly (10, 310) adapted for the winding of electrical coils is taught. A primary intended use is for use in particle tube assemblies (532) for the superconducting super collider. The correction coil cables (10, 310) have wires (14, 314) collected in wire arrays (12, 312) with a center rib (16, 316) sandwiched therebetween to form a core assembly (18, 318 ). The core assembly (18, 318) is surrounded by an assembly housing (20, 320) having an inner spiral wrap (22, 322) and a counter wound outer spiral wrap (24, 324). An alternate embodiment (410) of the invention is rolled into a keystoned shape to improve radial alignment of the correction coil cable (410) on a particle tube (733) in a particle tube assembly (732).

  15. Coiling of Elastic Ropes

    NASA Astrophysics Data System (ADS)

    Habibi, M.; Ribe, N. M.; Bonn, Daniel

    2007-10-01

    A rope falling onto a solid surface typically forms a series of regular coils. Here, we study this phenomenon using laboratory experiments (with cotton threads and softened spaghetti) and an asymptotic “slender-rope” numerical model. The excellent agreement between the two with no adjustable parameters allows us to determine a complete phase diagram for elastic coiling comprising three basic regimes involving different force balances (elastic, gravitational, and inertial) together with resonant “whirling string” and “whirling shaft” eigenmodes in the inertial regime.

  16. Imprinted Clay Coil Vessels

    ERIC Educational Resources Information Center

    Lohr, Tresa Rae

    2006-01-01

    The author teaches clay vessel construction in the fifth grade, and it is amazing what can be accomplished in one forty-five minute period when the expectations are clarified in the initial lesson. The author introduces clay coil vessels with a discussion of the sources of clay and how clay relates to fifth-grade science curriculum concepts such…

  17. Improved Coil for Hydrogen Dissociators

    NASA Technical Reports Server (NTRS)

    Vessot, R.

    1984-01-01

    Flat coil has rigid printed circuit substrate. New coil structure minimizes RF electric field near glass walls of plasma vessel; therefore reduces direct electron bombardment of glass. Design lends itself well to high production and standardized dimensions.

  18. Pulse Test of Coil Insulation

    NASA Technical Reports Server (NTRS)

    Kroy, Ralph E.

    1987-01-01

    Waveform of back-electromotive force reveals defects. Simple pulse test reveals defects in inductor coils. Devised for use on servovalve solenoid coils on Space Shuttle, test also applicable to transformer windings, chokes, relays, and the like.

  19. Magnetic Coil Design and Analysis

    NASA Astrophysics Data System (ADS)

    Bulatowicz, Michael

    2012-06-01

    Modified magnetic field coil geometries as described in U.S. Patent Applications US20100194506 and US20110247414 can produce substantially greater magnetic field homogeneity as compared to the traditional realized versions of idealized magnetic coil geometries such as spherical or Helmholtz. The new coil geometries will be described in detail and will be compared and contrasted to realized versions of idealized geometries, including discussion of errors not typically accounted for in traditional coil design and analysis.

  20. Wet Winding Improves Coil Encapsulation

    NASA Technical Reports Server (NTRS)

    Hill, A. J.

    1987-01-01

    Wet-winding process encapsulates electrical coils more uniformily than conventional processes. Process requires no vacuum pump and adapts easily to existing winding machines. Encapsulant applied to each layer of wire as soon as added to coil. Wet-winding process eliminates voids, giving more uniformly encapsulated coil.

  1. Design of printed circuit coils

    NASA Technical Reports Server (NTRS)

    Higgins, W. T.

    1969-01-01

    Spiral-like coil is printed with several extra turns which increase the realizable coil inductance. Included are shorting connections which not only short the extra turns, but also short out several turns of the main body. Coil tuning is accomplished by removing the shorts until the desired inductance is obtained.

  2. Coiled-coil networking shapes cell molecular machinery

    PubMed Central

    Wang, Yongqiang; Zhang, Xinlei; Zhang, Hong; Lu, Yi; Huang, Haolong; Dong, Xiaoxi; Chen, Jinan; Dong, Jiuhong; Yang, Xiao; Hang, Haiying; Jiang, Taijiao

    2012-01-01

    The highly abundant α-helical coiled-coil motif not only mediates crucial protein–protein interactions in the cell but is also an attractive scaffold in synthetic biology and material science and a potential target for disease intervention. Therefore a systematic understanding of the coiled-coil interactions (CCIs) at the organismal level would help unravel the full spectrum of the biological function of this interaction motif and facilitate its application in therapeutics. We report the first identified genome-wide CCI network in Saccharomyces cerevisiae, which consists of 3495 pair-wise interactions among 598 predicted coiled-coil regions. Computational analysis revealed that the CCI network is specifically and functionally organized and extensively involved in the organization of cell machinery. We further show that CCIs play a critical role in the assembly of the kinetochore, and disruption of the CCI network leads to defects in kinetochore assembly and cell division. The CCI network identified in this study is a valuable resource for systematic characterization of coiled coils in the shaping and regulation of a host of cellular machineries and provides a basis for the utilization of coiled coils as domain-based probes for network perturbation and pharmacological applications. PMID:22875988

  3. The Automotive Ignition Coil

    NASA Technical Reports Server (NTRS)

    Darnell, T H

    1932-01-01

    This report gives the results of a series of measurements on the secondary voltage induced in an ignition coil of typical construction under a variety of operating conditions. These results show that the theoretical predictions hitherto made as to the behavior of this type of apparatus are in satisfactory agreement with the observed facts. The large mass of data obtained is here published both for the use of other investigators who may wish to compare them with other theoretical predictions and for the use of automotive engineers who will here find definite experimental results showing the effect of secondary capacity and resistance on the crest voltage produced by ignition apparatus.

  4. Triple Halo Coil: Development and Comparison with Other TMS Coils

    NASA Astrophysics Data System (ADS)

    Rastogi, Priyam; Hadimani, Ravi; Jiles, David

    Transcranial Magnetic Stimulation (TMS) is a non-invasive stimulation technique that can be used for the treatment of various neurological disorders such as Parkinson's Disease, PTSD, TBI and anxiety by regulating synaptic activity. TMS is FDA approved for the treatment of major depressive disorder. There is a critical need to develop deep TMS coils that can stimulate deeper regions of the brain without excessively stimulating the cortex in order to provide an alternative to surgical methods. We have developed a novel multi-coil configuration called ``Triple Halo Coil'' (THC) that can stimulate deep brain regions. Investigation of induced electric and magnetic field in these regions have been achieved by computer modelling. Comparison of the results due to THC configuration have been conducted with other TMS coils such as ``Halo Coil'', circular coil and ``Figure of Eight'' coil. There was an improvement of more than 15 times in the strength of magnetic field, induced by THC configuration at 10 cm below the vertex of the head when compared with the ``Figure of Eight'' coil alone. Carver Charitable Trust.

  5. A Parallel Coiled-Coil Tetramer with Offset Helices

    SciTech Connect

    Liu,J.; Deng, Y.; Zheng, Q.; Cheng, C.; Kallenbach, N.; Lu, M.

    2006-01-01

    Specific helix-helix interactions are fundamental in assembling the native state of proteins and in protein-protein interfaces. Coiled coils afford a unique model system for elucidating principles of molecular recognition between {alpha} helices. The coiled-coil fold is specified by a characteristic seven amino acid repeat containing hydrophobic residues at the first (a) and fourth (d) positions. Nonpolar side chains spaced three and four residues apart are referred to as the 3-4 hydrophobic repeat. The presence of apolar amino acids at the e or g positions (corresponding to a 3-3-1 hydrophobic repeat) can provide new possibilities for close-packing of {alpha}-helices that includes examples such as the lac repressor tetramerization domain. Here we demonstrate that an unprecedented coiled-coil interface results from replacement of three charged residues at the e positions in the dimeric GCN4 leucine zipper by nonpolar valine side chains. Equilibrium circular dichroism and analytical ultracentrifugation studies indicate that the valine-containing mutant forms a discrete {alpha}-helical tetramer with a significantly higher stability than the parent leucine-zipper molecule. The 1.35 {angstrom} resolution crystal structure of the tetramer reveals a parallel four-stranded coiled coil with a three-residue interhelical offset. The local packing geometry of the three hydrophobic positions in the tetramer conformation is completely different from that seen in classical tetrameric structures yet bears resemblance to that in three-stranded coiled coils. These studies demonstrate that distinct van der Waals interactions beyond the a and d side chains can generate a diverse set of helix-helix interfaces and three-dimensional supercoil structures.

  6. Replaceable Sleeve Protects Welder Coil

    NASA Technical Reports Server (NTRS)

    Baker, W. L.; Simpson, C., E.

    1983-01-01

    New replaceable carbon insert for deflection coil in electron-beam welder promises to decrease maintenance costs. Inserts made from materials other than carbon (not yet tried) are less expensive, thus reducing costs even further. With carbon insert, deflection coils last longer and are easier to maintain.

  7. Magnet Coil Shorted Turn Detector

    SciTech Connect

    Dinkel, J.A.; Biggs, J.E.

    1994-03-01

    The Magnet Coil Shorted Turn Detector has been developed to facilitate the location of shorted turns in magnet coils. Finding these shorted turns is necessary to determine failure modes that are a necessary step in developing future production techniques. Up to this point, coils with shorted turns had the insulation burned off without the fault having been located. This disassembly process destroyed any chance of being able to find the fault. In order to maintain a flux balance in a coupled system such as a magnet coil, the current in a shorted turn must be opposed to the incident current. If the direction of the current in each conductor can be measured relative to the incident current, then the exact location of the short can be determined. In this device, an AC voltage is applied to the magnet under test. A small hand held B-dot pickup coil monitors the magnetic field produced by current in the individual magnet conductors. The relative phase of this pickup coil voltage is compared to a reference signal derived from the input current to detect a current reversal as the B-dot pickup coil is swept over the conductors of the coil under test. This technique however, is limited to only those conductors that are accessible to the hand held probe.

  8. The Coil Method in Contemporary Ceramics

    ERIC Educational Resources Information Center

    Nigrosh, Leon I.

    1976-01-01

    For centuries coil building has been the primary method of making pottery the world over. Many classrooms still reflect this preference for symmetrical coil building. Describes coil building and what forms can be made from it. (Author/RK)

  9. Coiled-coil intermediate filament stutter instability and molecular unfolding.

    PubMed

    Arslan, Melis; Qin, Zhao; Buehler, Markus J

    2011-05-01

    Intermediate filaments (IFs) are the key components of cytoskeleton in eukaryotic cells and are critical for cell mechanics. The building block of IFs is a coiled-coil alpha-helical dimer, consisting of several domains that include linkers and other structural discontinuities. One of the discontinuities in the dimer's coiled-coil region is the so-called 'stutter' region. The stutter is a region where a variation of the amino acid sequence pattern from other parts of the alpha-helical domains of the protein is found. It was suggested in earlier works that due to this sequence variation, the perfect coiled-coil arrangement ceases to exist. Here, we show using explicit water molecular dynamics and well-tempered metadynamics that for the coil2 domain of vimentin IFs the stutter is more stable in a non-alpha-helical, unfolded state. This causes a local structural disturbance in the alpha helix, which has a global effect on the nanomechanics of the structure. Our analysis suggests that the stutter features an enhanced tendency to unfolding even under the absence of external forces, implying a much greater structural instability than previously assumed. As a result it features a smaller local bending stiffness than other segments and presents a seed for the initiation of molecular bending and unfolding at large deformation. PMID:21516532

  10. Optimized quadrature surface coil designs

    PubMed Central

    Kumar, Ananda; Bottomley, Paul A.

    2008-01-01

    Background Quadrature surface MRI/MRS detectors comprised of circular loop and figure-8 or butterfly-shaped coils offer improved signal-to-noise-ratios (SNR) compared to single surface coils, and reduced power and specific absorption rates (SAR) when used for MRI excitation. While the radius of the optimum loop coil for performing MRI at depth d in a sample is known, the optimum geometry for figure-8 and butterfly coils is not. Materials and methods The geometries of figure-8 and square butterfly detector coils that deliver the optimum SNR are determined numerically by the electromagnetic method of moments. Figure-8 and loop detectors are then combined to create SNR-optimized quadrature detectors whose theoretical and experimental SNR performance are compared with a novel quadrature detector comprised of a strip and a loop, and with two overlapped loops optimized for the same depth at 3 T. The quadrature detection efficiency and local SAR during transmission for the three quadrature configurations are analyzed and compared. Results The SNR-optimized figure-8 detector has loop radius r8 ∼ 0.6d, so r8/r0 ∼ 1.3 in an optimized quadrature detector at 3 T. The optimized butterfly coil has side length ∼ d and crossover angle of ≥ 150° at the center. Conclusions These new design rules for figure-8 and butterfly coils optimize their performance as linear and quadrature detectors. PMID:18057975

  11. Improved Sensing Coils for SQUIDs

    NASA Technical Reports Server (NTRS)

    Penanen, Konstantin; Hahn, Inseob; Eom, Byeong Ho

    2007-01-01

    An improvement in the design and fabrication of sensing coils of superconducting quantum interference device (SQUID) magnetometers has been proposed to increase sensitivity. It has been estimated that, in some cases, it would be possible to increase sensitivity by about half or to reduce measurement time correspondingly. The pertinent aspects of the problems of design and fabrication can be summarized as follows: In general, to increase the sensitivity of a SQUID magnetometer, it is necessary to maximize the magnetic flux enclosed by the sensing coil while minimizing the self-inductance of this coil. It is often beneficial to fabricate the coil from a thicker wire to reduce its self-inductance. Moreover, to optimize the design of the coil with respect to sensitivity, it may be necessary to shape the wire to other than a commonly available circular or square cross-section. On the other hand, it is not practical to use thicker superconducting wire for the entire superconducting circuit, especially if the design of a specific device requires a persistent-current loop enclosing a remotely placed SQUID sensor. It may be possible to bond a thicker sensing-coil wire to thinner superconducting wires leading to a SQUID sensor, but it could be difficult to ensure reliable superconducting connections, especially if the bonded wires are made of different materials. The main idea is to mold the sensing coil in place, to more nearly optimum cross sectional shape, instead of making the coil by winding standard pre-fabricated wire. For this purpose, a thin superconducting wire loop that is an essential part of the SQUID magnetometer would be encapsulated in a form that would serve as a mold. A low-melting-temperature superconducting metal (e.g., indium, tin, or a lead/tin alloy) would be melted into the form, which would be sized and shaped to impart the required cross section to the coil thus formed.

  12. Coiling of a viscous filament

    NASA Astrophysics Data System (ADS)

    Samuel, A. D. T.; Ryu, W. S.; Mahadevan, L.

    1997-11-01

    A classic demonstration of fluid buckling is a daily occurence at the breakfast table, where a continuous stream of viscous fluid (honey) is often poured onto a flat surface (toast) from a sufficient height. The thin fluid filament quickly settles into a steady state; near the surface it bends into a helical shape while simultaneously rotating about the vertical and is laid out in a regular coil. This behavior is reminiscent of the coiling of a falling flexible rope. We derive a simple scaling law that predicts the coiling frequency in terms of the filament radius and the flow rate. We also verify this scaling law with the results of experiments.

  13. Laminated magnet field coil sheath

    DOEpatents

    Skaritka, John R.

    1987-12-01

    a method for manufacturing a magnet cable trim coil in a sheath assembly for use in a cryogenic particle accelerator. A precisely positioned pattern of trim coil turns is bonded to a flexible substrate sheath that is capable of withstanding cryogenic operating conditions. In the method of the invention the flexible sheath, with the trim coil pattern precisely positioned thereon, is accurately positioned at a precise location relative to a bore tube assembly of an accelerator and is then bonded to the bore tube with a tape suitable for cryogenic application. The resultant assembly can be readily handled and installed within an iron magnet yoke assembly of a suitable cryogenic particle accelerator.

  14. Laminated magnet field coil sheath

    DOEpatents

    Skaritka, J.R.

    1987-05-15

    A method for manufacturing a magnetic cable trim coil in a sheath assembly for use in a cryogenic particle accelerator. A precisely positioned pattern of trim coil turns is bonded to a flexible substrate sheath that is capable of withstanding cryogenic operating conditions. In the method of the invention the flexible substrate sheath, with the trim coil pattern precisely location relative to a bore tube assembly of an accelerator and is then bonded to the bore tube with a tape suitable for cryogenic application. The resultant assembly can be readily handled and installed within an iron magnet yoke assembly of a suitable cryogenic particle accelerator. 1 fig.

  15. ASTROMAG coil cooling study

    NASA Technical Reports Server (NTRS)

    Maytal, Ben-Zion; Vansciver, Steven W.

    1990-01-01

    ASTROMAG is a planned particle astrophysics magnetic facility. Basically it is a large magnetic spectrometer outside the Earth's atmosphere for an extended period of time in orbit on a space station. A definition team summarized its scientific objectives assumably related to fundamental questions of astrophysics, cosmology, and elementary particle physics. Since magnetic induction of about 7 Tesla is desired, it is planned to be a superconducting magnet cooled to liquid helium 2 temperatures. The general structure of ASTROMAG is based on: (1) two superconducting magnetic coils, (2) dewar of liquid helium 2 to provide cooling capability for the magnets; (3) instrumentation, matter-anti matter spectrometer (MAS) and cosmic ray isotope spectrometer (CRIS); and (4) interfaces to the shuttle and space station. Many configurations of the superconducting magnets and the dewar were proposed and evaluated, since those are the heart of the ASTROMAG. Baseline of the magnet configuration and cryostat as presented in the phase A study and the one kept in mind while doing the present study are presented. ASTROMAG's development schedule reflects the plan of launching to the space station in 1995.

  16. Growth factor identity is encoded by discrete coiled coil rotamers in the EGFR juxtamembrane region

    PubMed Central

    Doerner, Amy; Scheck, Rebecca; Schepartz, Alanna

    2015-01-01

    Summary Binding of the growth factor TGF-α to the EGFR extracellular domain is encoded through the formation of a unique anti-parallel coiled coil within the juxtamembrane segment. This new coiled coil is an ‘inside-out’ version of the coiled coil formed in the presence of EGF. A third, intermediary coiled coil interface is formed in the juxtamembrane segment when EGFR is stimulated with betacellulin. The seven growth factors that activate EGFR in mammalian systems (EGF, TGF-α, epigen, epiregulin, betacellulin, heparin-binding EGF, and amphiregulin) fall into distinct categories in which the structure of the coiled coil induced within the juxtamembrane segment correlates with cell state. The observation that coiled coil state tracks with the downstream signaling profiles for each ligand provides evidence for growth factor functional selectivity by EGFR. Encoding growth factor identity in alternative coiled coil rotamers provides a simple and elegant method for communicating chemical information across the plasma membrane. PMID:26091170

  17. High-resolution structures of a heterochiral coiled coil

    PubMed Central

    Mortenson, David E.; Steinkruger, Jay D.; Kreitler, Dale F.; Perroni, Dominic V.; Sorenson, Gregory P.; Huang, Lijun; Mittal, Ritesh; Yun, Hyun Gi; Travis, Benjamin R.; Mahanthappa, Mahesh K.; Forest, Katrina T.; Gellman, Samuel H.

    2015-01-01

    Interactions between polypeptide chains containing amino acid residues with opposite absolute configurations have long been a source of interest and speculation, but there is very little structural information for such heterochiral associations. The need to address this lacuna has grown in recent years because of increasing interest in the use of peptides generated from d amino acids (d peptides) as specific ligands for natural proteins, e.g., to inhibit deleterious protein–protein interactions. Coiled–coil interactions, between or among α-helices, represent the most common tertiary and quaternary packing motif in proteins. Heterochiral coiled–coil interactions were predicted over 50 years ago by Crick, and limited experimental data obtained in solution suggest that such interactions can indeed occur. To address the dearth of atomic-level structural characterization of heterochiral helix pairings, we report two independent crystal structures that elucidate coiled-coil packing between l- and d-peptide helices. Both structures resulted from racemic crystallization of a peptide corresponding to the transmembrane segment of the influenza M2 protein. Networks of canonical knobs-into-holes side-chain packing interactions are observed at each helical interface. However, the underlying patterns for these heterochiral coiled coils seem to deviate from the heptad sequence repeat that is characteristic of most homochiral analogs, with an apparent preference for a hendecad repeat pattern. PMID:26460035

  18. Adjustable Induction-Heating Coil

    NASA Technical Reports Server (NTRS)

    Ellis, Rod; Bartolotta, Paul

    1990-01-01

    Improved design for induction-heating work coil facilitates optimization of heating in different metal specimens. Three segments adjusted independently to obtain desired distribution of temperature. Reduces time needed to achieve required temperature profiles.

  19. Zero-angle helical coil

    NASA Technical Reports Server (NTRS)

    Troendle, J. A.

    1976-01-01

    Device is constructed of bimetallic stock material formed into segments of small diameters and fastened together by metal strips. Coil is useful in various types of actuators, such as temperature controls.

  20. Dynamics of liquid rope coiling

    NASA Astrophysics Data System (ADS)

    Habibi, Mehdi; Maleki, Maniya; Golestanian, Ramin; Ribe, Neil M.; Bonn, Daniel

    2006-12-01

    We present a combined experimental and numerical investigation of the coiling of a liquid “rope” falling on a solid surface, focusing on three little-explored aspects of the phenomenon: The time dependence of “inertio-gravitational” coiling, the systematic dependence of the radii of the coil and the rope on the experimental parameters, and the “secondary buckling” of the columnar structure generated by high-frequency coiling. Inertio-gravitational coiling is characterized by oscillations between states with different frequencies, and we present experimental observations of four distinct branches of such states in the frequency-fall height space. The transitions between coexisting states have no characteristic period, may take place with or without a change in the sense of rotation, and usually (but not always) occur via an intermediate “figure of eight” state. We present extensive laboratory measurements of the radii of the coil and of the rope within it, and show that they agree well with the predictions of a “slender-rope” numerical model. Finally, we use dimensional analysis to reveal a systematic variation of the critical column height for secondary buckling as a function of (dimensionless) flow rate and surface tension parameters.

  1. Nylon screws make inexpensive coil forms

    NASA Technical Reports Server (NTRS)

    Aucoin, G.; Rosenthal, C.

    1978-01-01

    Standard nylon screws act as coil form copper wire laid down in spiral thread. Completed coil may be bonded to printed-circuit board. However, it is impossible to tune coil by adjusting spacing between windings, technique sometimes used with air-core coils.

  2. Retrieval of Distally Migrated Coils with Detachable Intracranial Stent during Coil Embolization of Cerebral Aneurysm

    PubMed Central

    Singh, Devendra Pal; Huang, Lijin; Lee, Won Joo

    2016-01-01

    Migration of coils during endovascular procedures is a rare, but well-known complication. We are reporting two cases of successfully retrieving migrated coil using detachable intracranial stent. In both of our cases there was distal migration of coil during the intracranial aneurysm coiling procedure. The Solitaire® AB stent (Covidien, Irvine, CA, USA) was used to retrieve those coils. The stent was passed distal to the migrated coil using standard technique. It was then partially deployed and gradually withdrawn along with the entangled coil. Coil retrieval using the fully retrievable intracranial stent is a very simple, safe and easily available alternative for retrieval of distally migrated coil. PMID:27114967

  3. JET divertor coils, manufacture, assembly and testing

    NASA Astrophysics Data System (ADS)

    Dolgetta, N.; Bertolini, E.; D'Urzo, C.; Last, J. R.; Laurenti, A.; Presle, P.; Sannazzaro, G.; Tait, J.; Tesini, A.

    1994-07-01

    Four coils have been built and installed in the JET vacuum vessel to produce divertor plasmas. The coils are copper with glass epoxy insulation and are enclosed in vacuum tight Inconel cases. At the coil contractor's factory, the coil parts were manufactured and process techniques qualified. In the JET vacuum vessel the conductor bars were brazed to form the coils, which were inserted in the casings and impregnated and cured with epoxy resin.

  4. Tropomyosin lysine reactivities and relationship to coiled-coil structure.

    PubMed

    Hitchcock-DeGregori, S E; Lewis, S F; Chou, T M

    1985-06-18

    We have carried out a detailed analysis of tropomyosin structure using lysines as specific probes for the protein surface in regions of the molecule that have not been investigated by other methods. We have measured the relative reactivities of lysines in rabbit skeletal muscle alpha, alpha-tropomyosin with acetic anhydride using a competitive labeling procedure. We have identified 37 of 39 lysines and find that they range 20-fold in reactivity. The observed reactivities are related to the coiled-coil model of the tropomyosin molecule [Crick, F.H.C. (1953) Acta Crystallogr. 6, 689-697; McLachlan, A.D., Stewart, M., & Smillie, L.B. (1975) J. Mol. Biol. 98, 281-291] and other available chemical and physical information about the structure. In most cases, the observed lysine reactivities can be explained by allowable interactions with neighboring amino acid side chains on the same or facing alpha-helix. However, we found no correlation between reactivity and helical position of a given lysine. For example, lysines in the outer helical positions included lysines of low as well as high reactivity, indicating that they vary widely in their accessibility to solvent and that the coiled coil is heterogeneous along its length. Furthermore, the middle of the molecule (residues 126-182) that is susceptible to proteolysis and known to be the least stable region of the protein also contains some of the least and most reactive lysines. We have discussed the implications of our results on our understanding the structures of tropomyosin and other coiled-coil proteins as well as globular proteins containing helical regions. PMID:3927977

  5. Designed coiled coils promote folding of a recombinant bacterial collagen.

    PubMed

    Yoshizumi, Ayumi; Fletcher, Jordan M; Yu, Zhuoxin; Persikov, Anton V; Bartlett, Gail J; Boyle, Aimee L; Vincent, Thomas L; Woolfson, Derek N; Brodsky, Barbara

    2011-05-20

    Collagen triple helices fold slowly and inefficiently, often requiring adjacent globular domains to assist this process. In the Streptococcus pyogenes collagen-like protein Scl2, a V domain predicted to be largely α-helical, occurs N-terminal to the collagen triple helix (CL). Here, we replace this natural trimerization domain with a de novo designed, hyperstable, parallel, three-stranded, α-helical coiled coil (CC), either at the N terminus (CC-CL) or the C terminus (CL-CC) of the collagen domain. CD spectra of the constructs are consistent with additivity of independently and fully folded CC and CL domains, and the proteins retain their distinctive thermal stabilities, CL at ∼37 °C and CC at >90 °C. Heating the hybrid proteins to 50 °C unfolds CL, leaving CC intact, and upon cooling, the rate of CL refolding is somewhat faster for CL-CC than for CC-CL. A construct with coiled coils on both ends, CC-CL-CC, retains the ∼37 °C thermal stability for CL but shows less triple helix at low temperature and less denaturation at 50 °C. Most strikingly however, in CC-CL-CC, the CL refolds slower than in either CC-CL or CL-CC by almost two orders of magnitude. We propose that a single CC promotes folding of the CL domain via nucleation and in-register growth from one end, whereas initiation and growth from both ends in CC-CL-CC results in mismatched registers that frustrate folding. Bioinformatics analysis of natural collagens lends support to this because, where present, there is generally only one coiled-coil domain close to the triple helix, and it is nearly always N-terminal to the collagen repeat. PMID:21454493

  6. Transient voltage oscillations in coils

    SciTech Connect

    Chowdhuri, P.

    1985-01-01

    Magnet coils may be excited into internal voltage oscillations by transient voltages. Such oscillations may electrically stress the magnet's dielectric components to many times its normal stress. This may precipitate a dielectric failure, and the attendant prolonged loss of service and costly repair work. Therefore, it is important to know the natural frequencies of oscillations of a magnet during the design stage, and to determine whether the expected switching transient voltages can excite the magnet into high-voltage internal oscillations. The series capacitance of a winding significantly affects its natural frequencies. However, the series capacitance is difficult to calculate, because it may comprise complex capacitance network, consisting of intra- and inter-coil turn-to-turn capacitances of the coil sections. A method of calculating the series capacitance of a winding is proposed. This method is rigorous but simple to execute. The time-varying transient voltages along the winding are also calculated.

  7. The structure of the GemC1 coiled coil and its interaction with the Geminin family of coiled-coil proteins

    SciTech Connect

    Caillat, Christophe; Fish, Alexander; Pefani, Dafni-Eleftheria; Taraviras, Stavros; Lygerou, Zoi; Perrakis, Anastassis

    2015-10-31

    The GemC1 coiled-coil structure has subtle differences compared with its homologues Geminin and Idas. Co-expression experiments in cells and biophysical stability analysis of the Geminin-family coiled coils suggest that the GemC1 coiled coil alone is unstable. GemC1, together with Idas and Geminin, an important regulator of DNA-replication licensing and differentiation decisions, constitute a superfamily sharing a homologous central coiled-coil domain. To better understand this family of proteins, the crystal structure of a GemC1 coiled-coil domain variant engineered for better solubility was determined to 2.2 Å resolution. GemC1 shows a less typical coiled coil compared with the Geminin homodimer and the Geminin–Idas heterodimer structures. It is also shown that both in vitro and in cells GemC1 interacts with Geminin through its coiled-coil domain, forming a heterodimer that is more stable that the GemC1 homodimer. Comparative analysis of the thermal stability of all of the possible superfamily complexes, using circular dichroism to follow the unfolding of the entire helix of the coiled coil, or intrinsic tryptophan fluorescence of a unique conserved N-terminal tryptophan, shows that the unfolding of the coiled coil is likely to take place from the C-terminus towards the N-terminus. It is also shown that homodimers show a single-state unfolding, while heterodimers show a two-state unfolding, suggesting that the dimer first falls apart and the helices then unfold according to the stability of each protein. The findings argue that Geminin-family members form homodimers and heterodimers between them, and this ability is likely to be important for modulating their function in cycling and differentiating cells.

  8. Electromagnetic Gun With Commutated Coils

    NASA Technical Reports Server (NTRS)

    Elliott, David G.

    1991-01-01

    Proposed electromagnetic gun includes electromagnet coil, turns of which commutated in sequence along barrel. Electrical current fed to two armatures by brushes sliding on bus bars in barrel. Interaction between armature currents and magnetic field from coil produces force accelerating armature, which in turn, pushes on projectile. Commutation scheme chosen so magnetic field approximately coincides and moves with cylindrical region defined by armatures. Scheme has disadvantage of complexity, but in return, enables designer to increase driving magnetic field without increasing armature current. Attainable muzzle velocity increased substantially.

  9. [Two Cases of Ruptured Cerebral Aneurysm Complicated with Delayed Coil Protrusion after Coil Embolization].

    PubMed

    Furukawa, Takashi; Ogata, Atsushi; Ebashi, Ryo; Takase, Yukinori; Masuoka, Jun; Kawashima, Masatou; Abe, Tatsuya

    2016-07-01

    We report two cases of delayed coil protrusion after coil embolization for ruptured cerebral aneurysms. Case 1:An 82-year-old woman with a subarachnoid hemorrhage due to a ruptured small anterior communicating artery aneurysm underwent successful coil embolization. Eighteen days after the procedure, coil protrusion from the aneurysm into the right anterior cerebral artery was observed without any symptoms. Further coil protrusion did not develop after 28 days. Case 2:A 78-year-old woman with a subarachnoid hemorrhage due to a ruptured small left middle cerebral artery aneurysm underwent successful coil embolization. Twenty days after the procedure, coil protrusion from the aneurysm into the left middle cerebral artery was observed, with a transient ischemic attack. Further coil protrusion did not develop. Both patients recovered with antithrombotic treatment. Even though delayed coil protrusion after coil embolization is rare, it should be recognized as a long-term complication of coil embolization for cerebral aneurysms. PMID:27384117

  10. Finite element coiled cochlea model

    NASA Astrophysics Data System (ADS)

    Isailovic, Velibor; Nikolic, Milica; Milosevic, Zarko; Saveljic, Igor; Nikolic, Dalibor; Radovic, Milos; Filipović, Nenad

    2015-12-01

    Cochlea is important part of the hearing system, and thanks to special structure converts external sound waves into neural impulses which go to the brain. Shape of the cochlea is like snail, so geometry of the cochlea model is complex. The simplified cochlea coiled model was developed using finite element method inside SIFEM FP7 project. Software application is created on the way that user can prescribe set of the parameters for spiral cochlea, as well as material properties and boundary conditions to the model. Several mathematical models were tested. The acoustic wave equation for describing fluid in the cochlea chambers - scala vestibuli and scala timpani, and Newtonian dynamics for describing vibrations of the basilar membrane are used. The mechanical behavior of the coiled cochlea was analyzed and the third chamber, scala media, was not modeled because it does not have a significant impact on the mechanical vibrations of the basilar membrane. The obtained results are in good agreement with experimental measurements. Future work is needed for more realistic geometry model. Coiled model of the cochlea was created and results are compared with initial simplified coiled model of the cochlea.

  11. Wedding ring shaped excitation coil

    DOEpatents

    MacLennan, Donald A.; Tsai, Peter

    2001-01-01

    A high frequency inductively coupled electrodeless lamp includes an excitation coil with an effective electrical length which is less than one half wavelength of a driving frequency applied thereto, preferably much less. The driving frequency may be greater than 100 MHz and is preferably as high as 915 MHz. Preferably, the excitation coil is configured as a non-helical, semi-cylindrical conductive surface having less than one turn, in the general shape of a wedding ring. At high frequencies, the current in the coil forms two loops which are spaced apart and parallel to each other. Configured appropriately, the coil approximates a Helmholtz configuration. The lamp preferably utilizes an bulb encased in a reflective ceramic cup with a pre-formed aperture defined therethrough. The ceramic cup may include structural features to aid in alignment and/or a flanged face to aid in thermal management. The lamp head is preferably an integrated lamp head comprising a metal matrix composite surrounding an insulating ceramic with the excitation integrally formed on the ceramic. A novel solid-state oscillator preferably provides RF power to the lamp. The oscillator is a single active element device capable of providing over 70 watts of power at over 70% efficiency.

  12. Coupled Coils, Magnets and Lenz's Law

    ERIC Educational Resources Information Center

    Thompson, Frank

    2010-01-01

    Great scientists in the past have experimented with coils and magnets. Here we have a variation where coupling occurs between two coils and the oscillatory motion of two magnets to give somewhat surprising results. (Contains 6 figures and 1 footnote.)

  13. Bow-shaped toroidal field coils

    SciTech Connect

    Bonanos, P.

    1981-05-01

    Design features of Bow-Shaped Toroidal Field Coils are described and compared with circular and D shaped coils. The results indicate that bow coils can produce higher field strengths, store more energy and be made demountable. The design offers the potential for the production of ultrahigh toroidal fields. Included are representative coil shapes and their engineering properties, a suggested structural design and an analysis of a specific case.

  14. Multi-Canted Coils, Tubes, and Structures

    NASA Technical Reports Server (NTRS)

    Jaster, Mark L. (Inventor)

    2015-01-01

    Coil, tube, and other structures configured with a plurality of individual coils, internal structures, legs or extensions with each having multiple cants per coil, internal structure, leg or extension, and wherein the cants formed therein allow for a load-deflection force when each is compressed. In addition, any horizontal or moment forces are substantially reduced and/or eliminated when a downward vertical force is applied, as minimal or no torsion is created in the individual coils, legs or extensions.

  15. Design and modelling of a SMES coil

    NASA Astrophysics Data System (ADS)

    Yuan, Weijia; Campbell, A. M.; Coombs, T. A.

    2010-06-01

    The design of a Superconducting Magnetic Energy Storage (SMES) coil wound by coated conductors has been presented. Based on an existing model for coated conductor pancake coils, this paper analysed the magnetic field and current density distribution of the coil at two different operation temperatures, 77K and 22K. A comparison table of the critical currents and AC losses at these two temperatures has been presented. Several steps to improve the transport current of the coil have been suggested as well.

  16. Rotor assembly including superconducting magnetic coil

    DOEpatents

    Snitchler, Gregory L.; Gamble, Bruce B.; Voccio, John P.

    2003-01-01

    Superconducting coils and methods of manufacture include a superconductor tape wound concentrically about and disposed along an axis of the coil to define an opening having a dimension which gradually decreases, in the direction along the axis, from a first end to a second end of the coil. Each turn of the superconductor tape has a broad surface maintained substantially parallel to the axis of the coil.

  17. Split Coil Forms for Rotary Transformers

    NASA Technical Reports Server (NTRS)

    Mclyman, C. W. T.

    1982-01-01

    Split cores for rotor and stator windings of rotary transformer mounted around their respective coils (which are in bobbins) and cemented together. This arrangement simplifies winding of stator coil to go in a slot in inner diameter of stator coil. One practical application of rotary transformers fabricated according to this technique is for centrifuges, in which conventional sliprings are of uncertain reliability.

  18. Inductively coupled wireless RF coil arrays.

    PubMed

    Bulumulla, S B; Fiveland, E; Park, K J; Foo, T K; Hardy, C J

    2015-04-01

    As the number of coils increases in multi-channel MRI receiver-coil arrays, RF cables and connectors become increasingly bulky and heavy, degrading patient comfort and slowing workflow. Inductive coupling of signals provides an attractive "wireless" approach, with the potential to reduce coil weight and cost while simplifying patient setup. In this work, multi-channel inductively coupled anterior arrays were developed and characterized for 1.5T imaging. These comprised MR receiver coils inductively (or "wirelessly") linked to secondary or "sniffer" coils whose outputs were transmitted via preamps to the MR system cabinet. The induced currents in the imaging coils were blocked by passive diode circuits during RF transmit. The imaging arrays were totally passive, obviating the need to deliver power to the coils, and providing lightweight, untethered signal reception with easily positioned coils. Single-shot fast spin echo images were acquired from 5 volunteers using a 7-element inductively coupled coil array and a conventionally cabled 7-element coil array of identical geometry, with the inductively-coupled array showing a relative signal-to-noise ratio of 0.86 +/- 0.07. The concept was extended to a larger 9-element coil array to demonstrate the effect of coil element size on signal transfer and RF-transmit blocking. PMID:25523607

  19. Three-dimensional coil inductor

    DOEpatents

    Bernhardt, Anthony F.; Malba, Vincent

    2002-01-01

    A three-dimensional coil inductor is disclosed. The inductor includes a substrate; a set of lower electrically conductive traces positioned on the substrate; a core placed over the lower traces; a set of side electrically conductive traces laid on the core and the lower traces; and a set of upper electrically conductive traces attached to the side traces so as to form the inductor. Fabrication of the inductor includes the steps of forming a set of lower traces on a substrate; positioning a core over the lower traces; forming a set of side traces on the core; connecting the side traces to the lower traces; forming a set of upper traces on the core; and connecting the upper traces to the side traces so as to form a coil structure.

  20. Coil Embolization for Intracranial Aneurysms

    PubMed Central

    2006-01-01

    Executive Summary Objective To determine the effectiveness and cost-effectiveness of coil embolization compared with surgical clipping to treat intracranial aneurysms. The Technology Endovascular coil embolization is a percutaneous approach to treat an intracranial aneurysm from within the blood vessel without the need of a craniotomy. In this procedure, a microcatheter is inserted into the femoral artery near the groin and navigated to the site of the aneurysm. Small helical platinum coils are deployed through the microcatheter to fill the aneurysm, and prevent it from further expansion and rupture. Health Canada has approved numerous types of coils and coil delivery systems to treat intracranial aneurysms. The most favoured are controlled detachable coils. Coil embolization may be used with other adjunct endovascular devices such as stents and balloons. Background Intracranial Aneurysms Intracranial aneurysms are the dilation or ballooning of part of a blood vessel in the brain. Intracranial aneurysms range in size from small (<12 mm in diameter) to large (12–25 mm), and to giant (>25 mm). There are 3 main types of aneurysms. Fusiform aneurysms involve the entire circumference of the artery; saccular aneurysms have outpouchings; and dissecting aneurysms have tears in the arterial wall. Berry aneurysms are saccular aneurysms with well-defined necks. Intracranial aneurysms may occur in any blood vessel of the brain; however, they are most commonly found at the branch points of large arteries that form the circle of Willis at the base of the brain. In 85% to 95% of patients, they are found in the anterior circulation. Aneurysms in the posterior circulation are less frequent, and are more difficult to treat surgically due to inaccessibility. Most intracranial aneurysms are small and asymptomatic. Large aneurysms may have a mass effect, causing compression on the brain and cranial nerves and neurological deficits. When an intracranial aneurysm ruptures and bleeds

  1. Segmented Coil Fails In Steps

    NASA Technical Reports Server (NTRS)

    Stedman, Ronald S.

    1990-01-01

    Electromagnetic coil degrades in steps when faults occur, continues to operate at reduced level instead of failing catastrophically. Made in segments connected in series and separated by electrically insulating barriers. Fault does not damage adjacent components or create hazard. Used to control valves in such critical applications as cooling systems of power generators and chemical process equipment, where flammable liquids or gases handled. Also adapts to electrical control of motors.

  2. Equations determine coiled tubing collapse pressure

    SciTech Connect

    Avakov, V.; Taliaferro, W.

    1995-07-24

    A set of equations has been developed for calculating pipe collapse pressure for oval tubing such as coiled tubing. When coiled tubing is placed onto a reel, the tubing is forced into an oval shape and never again returns to perfect roundness because the coiling process exceeds the plasticity limits of the tubing. Straightening the tubing for the trip into the well does not restore roundness. The consequence of this physical property is that all coiled tubing collapse pressure calculations should be made considering oval tubing, not round tubing. Tubing collapse can occur when formation pressure against the coiled tubing exceeds the collapse resistance inherent in the coiled tubing. As coiled tubing becomes more oval in shape, it becomes more oval in shape, it becomes more susceptible to collapse from outside pressure.

  3. Coiled transmission line pulse generators

    DOEpatents

    McDonald, Kenneth Fox

    2010-11-09

    Methods and apparatus are provided for fabricating and constructing solid dielectric "Coiled Transmission Line" pulse generators in radial or axial coiled geometries. The pour and cure fabrication process enables a wide variety of geometries and form factors. The volume between the conductors is filled with liquid blends of monomers, polymers, oligomers, and/or cross-linkers and dielectric powders; and then cured to form high field strength and high dielectric constant solid dielectric transmission lines that intrinsically produce ideal rectangular high voltage pulses when charged and switched into matched impedance loads. Voltage levels may be increased by Marx and/or Blumlein principles incorporating spark gap or, preferentially, solid state switches (such as optically triggered thyristors) which produce reliable, high repetition rate operation. Moreover, these Marxed pulse generators can be DC charged and do not require additional pulse forming circuitry, pulse forming lines, transformers, or an a high voltage spark gap output switch. The apparatus accommodates a wide range of voltages, impedances, pulse durations, pulse repetition rates, and duty cycles. The resulting mobile or flight platform friendly cylindrical geometric configuration is much more compact, light-weight, and robust than conventional linear geometries, or pulse generators constructed from conventional components. Installing additional circuitry may accommodate optional pulse shape improvements. The Coiled Transmission Lines can also be connected in parallel to decrease the impedance, or in series to increase the pulse length.

  4. A thermodynamic model for the helix-coil transition coupled to dimerization of short coiled-coil peptides.

    PubMed Central

    Qian, H

    1994-01-01

    A simple thermodynamic formalism is presented to model the conformational transition between a random-coil monomeric peptide and a coiled-coil helical dimer. The coiled-coil helical dimer is the structure of a class of proteins also called leucine zipper, which has been studied intensively in recent years. Our model, which is appropriate particularly for short peptides, is an alternative to the theory developed by Skolnick and Holtzer. Using the present formalism, we discuss the multi-equilibriatory nature of this transition and provide an explanation for the apparent two-state behavior of coiled-coil formation when the helix-coil transition is coupled to dimerization. It is found that such coupling between multi-equilibria and a true two-state transition can simplify the data analysis, but care must be taken in using the overall association constant to determine helix propensities (w) of single residues. Successful use of the two-state model does not imply that the helix-coil transition is all-or-none. The all-or-none assumption can provide good numerical estimates when w is around unity (0.35 < or = w < or = 1.35), but when w is small (w < 0.01), similar estimations can lead to large errors. The theory of the helix-coil transition in denaturation experiments is also discussed. PMID:7919005

  5. A comparison of coupling efficiencies for a Stix coil and an m equals 1 coil

    NASA Technical Reports Server (NTRS)

    Sigman, D. R.

    1972-01-01

    This theoretical and experimental study compares the ion-cyclotron wave generating characteristics of a Stix coil (which generates waves with azimuthal mode number m = 0) with those of a coil which produces primarily m = + or -1 ion-cyclotron modes. The theoretical work of J.E. Hipp, which predicted very good coupling for the m = 1 coil, was extended to determine the scaling laws for plasma column radius and coil wavelength. Experimentally, an m = 1 coil and an m = 0 coil were used to generate ion-cyclotron waves on a beam generated plasma column with electron density = 10 to the 12th power/cu cm. Coupling resonances with peak efficiencies of approximately 40 to 50 percent were measured for both coils in low power (approximately 10k W) experiments. For equal power transfer to the plasma, the m = 0 coil voltage was more than a factor of two greater than that for the m = 1 coil.

  6. A Mechanical Coil Insertion System for Endovascular Coil Embolization of Intracranial Aneurysms

    PubMed Central

    Haraguchi, K.; Miyachi, S.; Matsubara, N.; Nagano, Y.; Yamada, H.; Marui, N.; Sano, A.; Fujimoto, H.; Izumi, T.; Yamanouchi, T.; Asai, T.; Wakabayashi, T.

    2013-01-01

    Summary Like other fields of medicine, robotics and mechanization might be introduced into endovascular coil embolization of intracranial aneurysms for effective treatment. We have already reported that coil insertion force could be smaller and more stable when the coil delivery wire is driven mechanically at a constant speed. Another background is the difficulty in synchronizing operators' minds and hands when two operators control the microcatheter and the coil respectively. We have therefore developed a mechanical coil insertion system enabling a single operator to insert coils at a fixed speed while controlling the microcatheter. Using our new system, the operator manipulated the microcatheter with both hands and drove the coil using foot switches simultaneously. A delivery wire force sensor previously reported was used concurrently, allowing the operator to detect excessive stress on the wire. In vitro coil embolization was performed using three methods: simple mechanical advance of the coil; simple mechanical advance of the coil with microcatheter control; and driving (forward and backward) of the coil using foot switches in addition to microcatheter control. The system worked without any problems, and did not interfere with any procedures. In experimental coil embolization, delivery wire control using the foot switches as well as microcatheter manipulation helped to achieve successful insertion of coils. This system could offer the possibility of developing safer and more efficient coil embolization. Although we aim at total mechanization and automation of procedures in the future, microcatheter manipulation and synchronized delivery wire control are still indispensable using this system. PMID:23693038

  7. A mechanical coil insertion system for endovascular coil embolization of intracranial aneurysms.

    PubMed

    Haraguchi, K; Miyachi, S; Matsubara, N; Nagano, Y; Yamada, H; Marui, N; Sano, A; Fujimoto, H; Izumi, T; Yamanouchi, T; Asai, T; Wakabayashi, T

    2013-06-01

    Like other fields of medicine, robotics and mechanization might be introduced into endovascular coil embolization of intracranial aneurysms for effective treatment. We have already reported that coil insertion force could be smaller and more stable when the coil delivery wire is driven mechanically at a constant speed. Another background is the difficulty in synchronizing operators' minds and hands when two operators control the microcatheter and the coil respectively. We have therefore developed a mechanical coil insertion system enabling a single operator to insert coils at a fixed speed while controlling the microcatheter. Using our new system, the operator manipulated the microcatheter with both hands and drove the coil using foot switches simultaneously. A delivery wire force sensor previously reported was used concurrently, allowing the operator to detect excessive stress on the wire. In vitro coil embolization was performed using three methods: simple mechanical advance of the coil; simple mechanical advance of the coil with microcatheter control; and driving (forward and backward) of the coil using foot switches in addition to microcatheter control. The system worked without any problems, and did not interfere with any procedures. In experimental coil embolization, delivery wire control using the foot switches as well as microcatheter manipulation helped to achieve successful insertion of coils. This system could offer the possibility of developing safer and more efficient coil embolization. Although we aim at total mechanization and automation of procedures in the future, microcatheter manipulation and synchronized delivery wire control are still indispensable using this system. PMID:23693038

  8. Optimized Geometry for Superconducting Sensing Coils

    NASA Technical Reports Server (NTRS)

    Eom, Byeong Ho; Pananen, Konstantin; Hahn, Inseob

    2008-01-01

    An optimized geometry has been proposed for superconducting sensing coils that are used in conjunction with superconducting quantum interference devices (SQUIDs) in magnetic resonance imaging (MRI), magnetoencephalography (MEG), and related applications in which magnetic fields of small dipoles are detected. In designing a coil of this type, as in designing other sensing coils, one seeks to maximize the sensitivity of the detector of which the coil is a part, subject to geometric constraints arising from the proximity of other required equipment. In MRI or MEG, the main benefit of maximizing the sensitivity would be to enable minimization of measurement time. In general, to maximize the sensitivity of a detector based on a sensing coil coupled with a SQUID sensor, it is necessary to maximize the magnetic flux enclosed by the sensing coil while minimizing the self-inductance of this coil. Simply making the coil larger may increase its self-inductance and does not necessarily increase sensitivity because it also effectively increases the distance from the sample that contains the source of the signal that one seeks to detect. Additional constraints on the size and shape of the coil and on the distance from the sample arise from the fact that the sample is at room temperature but the coil and the SQUID sensor must be enclosed within a cryogenic shield to maintain superconductivity.

  9. Coiled-coil protein composition of 22 proteomes – differences and common themes in subcellular infrastructure and traffic control

    PubMed Central

    Rose, Annkatrin; Schraegle, Shannon J; Stahlberg, Eric A; Meier, Iris

    2005-01-01

    Background Long alpha-helical coiled-coil proteins are involved in diverse organizational and regulatory processes in eukaryotic cells. They provide cables and networks in the cyto- and nucleoskeleton, molecular scaffolds that organize membrane systems and tissues, motors, levers, rotating arms, and possibly springs. Mutations in long coiled-coil proteins have been implemented in a growing number of human diseases. Using the coiled-coil prediction program MultiCoil, we have previously identified all long coiled-coil proteins from the model plant Arabidopsis thaliana and have established a searchable Arabidopsis coiled-coil protein database. Results Here, we have identified all proteins with long coiled-coil domains from 21 additional fully sequenced genomes. Because regions predicted to form coiled-coils interfere with sequence homology determination, we have developed a sequence comparison and clustering strategy based on masking predicted coiled-coil domains. Comparing and grouping all long coiled-coil proteins from 22 genomes, the kingdom-specificity of coiled-coil protein families was determined. At the same time, a number of proteins with unknown function could be grouped with already characterized proteins from other organisms. Conclusion MultiCoil predicts proteins with extended coiled-coil domains (more than 250 amino acids) to be largely absent from bacterial genomes, but present in archaea and eukaryotes. The structural maintenance of chromosomes proteins and their relatives are the only long coiled-coil protein family clearly conserved throughout all kingdoms, indicating their ancient nature. Motor proteins, membrane tethering and vesicle transport proteins are the dominant eukaryote-specific long coiled-coil proteins, suggesting that coiled-coil proteins have gained functions in the increasingly complex processes of subcellular infrastructure maintenance and trafficking control of the eukaryotic cell. PMID:16288662

  10. De Novo Design of Ln(III) Coiled Coils for Imaging Applications

    PubMed Central

    2014-01-01

    A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)3. The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities. PMID:24405157

  11. The Golgin Family of Coiled-Coil Tethering Proteins

    PubMed Central

    Witkos, Tomasz M.; Lowe, Martin

    2016-01-01

    The golgins are a family of predominantly coiled-coil proteins that are localized to the Golgi apparatus. Golgins are present in all eukaryotes, suggesting an evolutionary conserved function. Golgins are anchored to the Golgi membrane by their carboxy terminus and are predicted to adopt an extended conformation that projects into the surrounding cytoplasm. This arrangement is ideal for the capture or tethering of nearby membranes or cytoskeletal elements. Golgin-mediated tethering is thought to be important for vesicular traffic at the Golgi apparatus, the maintenance of Golgi architecture, as well as the positioning of the Golgi apparatus within cells. In addition to acting as tethers, some golgins can also sequester various factors at the Golgi membrane, allowing for the spatiotemporal regulation of downstream cellular functions. Although it is now established that golgins are membrane and cytoskeleton tethers, the mechanisms underlying tethering remain poorly defined. Moreover, the importance of golgin-mediated tethering in a physiological context remains to be fully explored. This review will describe our current understanding of golgin function, highlighting recent progress that has been made, and goes on to discuss outstanding questions and potential avenues for future research with regard to this family of conserved Golgi-associated proteins. PMID:26793708

  12. Current COIL research in Samara

    NASA Astrophysics Data System (ADS)

    Nikolaev, Valeri D.

    1996-02-01

    Development of the high pressure singlet oxygen generator (SOG) is a very important aspect for chemical oxygen-iodine laser (COIL). Increasing of oxygen pressure up to 30 torr and more at conserving high O2(1(Delta) ) yield and maintaining BHP temperature at minus (10 divided by 20) degrees Celsius permits us to decrease ration [H2O]/[O2] to 5% and less. In this case COIL can operate successfully without a water vapor trap. With raising the total pressure Reynolds number increases too, diminishing boundary layers in supersonic nozzles and improving pressure recovery. The weight and dimensions of the SOG and laser become reduced for the same gas flow rate. For solving these problems the jet SOG has been suggested and developed in Lebedev Physical Institute, Samara Branch. The advantages of the jet SOG consist of the following: (1) Large and controlled specific surface of contact liquid-gas provides for high mass transfer efficiency. (2) High jets velocity guarantees fast basic hydrogen peroxide (BHP) surface renovation. (3) High gas velocity in the reaction zone diminishes O2(1(Delta) ) quenching. (4) Efficient gas-liquid heat exchange eliminates the gas heating and generation water vapor due O2(1(Delta) ) quenching. (5) Counterflowing design of the jet SOG produces the best conditions for self-cleaning gas flow of droplets in the reaction zone and gives the possibility of COIL operation without droplets separator. High pressure jet SOG has some features connected with intrachannel jet formation, free space jets reconstruction, interaction jets ensemble with counter moving gas flow and drag part of gas by jets, disintegrating jets, generation and separation of droplets, heat effects, surface renovation, impoverishment BHP surface by HO2- ions, moving solution film on the reaction zone walls, etc. In this communication our current understanding of the major processes in the jet SOG is set forth. The complex gas and hydrodynamic processes with heat and mass transfer

  13. Development of a new error field correction coil (C-coil) for DIII-D

    SciTech Connect

    Robinson, J.I.; Scoville, J.T.

    1995-12-01

    The C-coil recently installed on the DIII-D tokamak was developed to reduce the error fields created by imperfections in the location and geometry of the existing coils used to confine, heat, and shape the plasma. First results from C-coil experiments include stable operation in a 1.6 MA plasma with a density less than 1.0 {times} 10{sup 13} cm{sup {minus}3}, nearly a factor of three lower density than that achievable without the C-coil. The C-coil has also been used in magnetic braking of the plasma rotation and high energy particle confinement experiments. The C-coil system consists of six individual saddle coils, each 60{degree} wide toroidally, spanning the midplane of the vessel with a vertical height of 1.6 m. The coils are located at a major radius of 3.2 m, just outside of the toroidal field coils. The actual shape and geometry of each coil section varied somewhat from the nominal dimensions due to the large number of obstructions to the desired coil path around the already crowded tokamak. Each coil section consists of four turns of 750 MCM insulated copper cable banded with stainless steel straps within the web of a 3 in. x 3 in. stainless steel angle frame. The C-coil structure was designed to resist peak transient radial forces (up to 1,800 Nm) exerted on the coil by the toroidal and ploidal fields. The coil frames were supported from existing poloidal field coil case brackets, coil studs, and various other structures on the tokamak.

  14. Critical evaluation of in silico methods for prediction of coiled-coil domains in proteins.

    PubMed

    Li, Chen; Ching Han Chang, Catherine; Nagel, Jeremy; Porebski, Benjamin T; Hayashida, Morihiro; Akutsu, Tatsuya; Song, Jiangning; Buckle, Ashley M

    2016-03-01

    Coiled-coils refer to a bundle of helices coiled together like strands of a rope. It has been estimated that nearly 3% of protein-encoding regions of genes harbour coiled-coil domains (CCDs). Experimental studies have confirmed that CCDs play a fundamental role in subcellular infrastructure and controlling trafficking of eukaryotic cells. Given the importance of coiled-coils, multiple bioinformatics tools have been developed to facilitate the systematic and high-throughput prediction of CCDs in proteins. In this article, we review and compare 12 sequence-based bioinformatics approaches and tools for coiled-coil prediction. These approaches can be categorized into two classes: coiled-coil detection and coiled-coil oligomeric state prediction. We evaluated and compared these methods in terms of their input/output, algorithm, prediction performance, validation methods and software utility. All the independent testing data sets are available at http://lightning.med.monash.edu/coiledcoil/. In addition, we conducted a case study of nine human polyglutamine (PolyQ) disease-related proteins and predicted CCDs and oligomeric states using various predictors. Prediction results for CCDs were highly variable among different predictors. Only two peptides from two proteins were confirmed to be CCDs by majority voting. Both domains were predicted to form dimeric coiled-coils using oligomeric state prediction. We anticipate that this comprehensive analysis will be an insightful resource for structural biologists with limited prior experience in bioinformatics tools, and for bioinformaticians who are interested in designing novel approaches for coiled-coil and its oligomeric state prediction. PMID:26177815

  15. Crystal Structure of a Super Leucine Zipper an Extended Two-Stranded Super Long Coiled Coil

    SciTech Connect

    J Diao

    2011-12-31

    Coiled coil is a ubiquitous structural motif in proteins, with two to seven alpha helices coiled together like the strands of a rope, and coiled coil folding and assembly is not completely understood. A GCN4 leucine zipper mutant with four mutations of K3A, D7A, Y17W, and H18N has been designed, and the crystal structure has been determined at 1.6 {angstrom} resolution. The peptide monomer shows a helix trunk with short curved N- and C-termini. In the crystal, two monomers cross in 35{sup o} and form an X-shaped dimer, and each X-shaped dimer is welded into the next one through sticky hydrophobic ends, thus forming an extended two-stranded, parallel, super long coiled coil rather than a discrete, two-helix coiled coil of the wild-type GCN4 leucine zipper. Leucine residues appear at every seventh position in the super long coiled coil, suggesting that it is an extended super leucine zipper. Compared to the wild-type leucine zipper, the N-terminus of the mutant has a dramatic conformational change and the C-terminus has one more residue Glu 32 determined. The mutant X-shaped dimer has a large crossing angle of 35{sup o} instead of 18{sup o} in the wild-type dimer. The results show a novel assembly mode and oligomeric state of coiled coil, and demonstrate that mutations may affect folding and assembly of the overall coiled coil. Analysis of the formation mechanism of the super long coiled coil may help understand and design self-assembling protein fibers.

  16. Electromagnetic levitation coil fabrication technique for MSFC containerless processing facilities

    NASA Technical Reports Server (NTRS)

    Ethridge, E. C.; Theiss, J.; Curreri, P. A.; Abbaschian, G. J.

    1983-01-01

    A technique is described for more reproducible fabrication of electromagnetic levitation coils. A split mandrel was developed upon which the coil is wound. After fabrication the mandrel can be disassembled to remove it from the coil. Previously, a full day was required to fabricate a levitation coil and the success rate for a functional coil was only 50 percent. About eight coils may be completed in one day using the technique developed and 95 percent of them are good levitation coils.

  17. The structure of the GemC1 coiled coil and its interaction with the Geminin family of coiled-coil proteins

    PubMed Central

    Caillat, Christophe; Fish, Alexander; Pefani, Dafni-Eleftheria; Taraviras, Stavros; Lygerou, Zoi; Perrakis, Anastassis

    2015-01-01

    GemC1, together with Idas and Geminin, an important regulator of DNA-replication licensing and differentiation decisions, constitute a superfamily sharing a homologous central coiled-coil domain. To better understand this family of proteins, the crystal structure of a GemC1 coiled-coil domain variant engineered for better solubility was determined to 2.2 Å resolution. GemC1 shows a less typical coiled coil compared with the Geminin homodimer and the Geminin–Idas heterodimer structures. It is also shown that both in vitro and in cells GemC1 interacts with Geminin through its coiled-coil domain, forming a heterodimer that is more stable that the GemC1 homodimer. Comparative analysis of the thermal stability of all of the possible superfamily complexes, using circular dichroism to follow the unfolding of the entire helix of the coiled coil, or intrinsic tryptophan fluorescence of a unique conserved N-terminal tryptophan, shows that the unfolding of the coiled coil is likely to take place from the C-terminus towards the N-terminus. It is also shown that homodimers show a single-state unfolding, while heterodimers show a two-state unfolding, suggesting that the dimer first falls apart and the helices then unfold according to the stability of each protein. The findings argue that Geminin-family members form homodimers and heterodimers between them, and this ability is likely to be important for modulating their function in cycling and differentiating cells. PMID:26527144

  18. Transient response of coaxial pulse coils

    NASA Astrophysics Data System (ADS)

    Clifton, S.; Mongeau, P.

    1984-03-01

    Of central importance in designing coaxial launcher systems is understanding the mechanical response and structural limits of the magnetic pulse coils. In normal operation the driving frequency can vary from static conditions through the lowest natural modes to well beyond the highest frequencies. By using a lumped parameter model the transient behavior of a magnetic pulse coil can be readily characterized. In an effort to understand the failure mechanism of coaxial pulse coils the results of this model are compared to the experimental performance of several thin build coils.

  19. A precise technique for manufacturing correction coil

    SciTech Connect

    Schieber, L.

    1992-11-01

    An automated method of manufacturing correction coils has been developed which provides a precise embodiment of the coil design. Numerically controlled machines have been developed to accurately position coil windings on the beam tube. Two types of machines have been built. One machine bonds the wire to a substrate which is wrapped around the beam tube after it is completed while the second machine bonds the wire directly to the beam tube. Both machines use the Multiwire{reg_sign} technique of bonding the wire to the substrate utilizing an ultrasonic stylus. These machines are being used to manufacture coils for both the SSC and RHIC.

  20. A precise technique for manufacturing correction coil

    SciTech Connect

    Schieber, L.

    1992-01-01

    An automated method of manufacturing correction coils has been developed which provides a precise embodiment of the coil design. Numerically controlled machines have been developed to accurately position coil windings on the beam tube. Two types of machines have been built. One machine bonds the wire to a substrate which is wrapped around the beam tube after it is completed while the second machine bonds the wire directly to the beam tube. Both machines use the Multiwire[reg sign] technique of bonding the wire to the substrate utilizing an ultrasonic stylus. These machines are being used to manufacture coils for both the SSC and RHIC.

  1. Defect-Free Carbon Nanotube Coils.

    PubMed

    Shadmi, Nitzan; Kremen, Anna; Frenkel, Yiftach; Lapin, Zachary J; Machado, Leonardo D; Legoas, Sergio B; Bitton, Ora; Rechav, Katya; Popovitz-Biro, Ronit; Galvão, Douglas S; Jorio, Ado; Novotny, Lukas; Kalisky, Beena; Joselevich, Ernesto

    2016-04-13

    Carbon nanotubes are promising building blocks for various nanoelectronic components. A highly desirable geometry for such applications is a coil. However, coiled nanotube structures reported so far were inherently defective or had no free ends accessible for contacting. Here we demonstrate the spontaneous self-coiling of single-wall carbon nanotubes into defect-free coils of up to more than 70 turns with identical diameter and chirality, and free ends. We characterize the structure, formation mechanism, and electrical properties of these coils by different microscopies, molecular dynamics simulations, Raman spectroscopy, and electrical and magnetic measurements. The coils are highly conductive, as expected for defect-free carbon nanotubes, but adjacent nanotube segments in the coil are more highly coupled than in regular bundles of single-wall carbon nanotubes, owing to their perfect crystal momentum matching, which enables tunneling between the turns. Although this behavior does not yet enable the performance of these nanotube coils as inductive devices, it does point a clear path for their realization. Hence, this study represents a major step toward the production of many different nanotube coil devices, including inductors, electromagnets, transformers, and dynamos. PMID:26708150

  2. Helical axis stellarator with noninterlocking planar coils

    DOEpatents

    Reiman, Allan; Boozer, Allen H.

    1987-01-01

    A helical axis stellarator using only noninterlocking planar, non-circular coils, generates magnetic fields having a magnetic well and large rotational transform with resultant large equilibrium beta.

  3. Self-correction coil: Operation mechanism of self-correction coil

    NASA Astrophysics Data System (ADS)

    Hosoyama, K.

    1983-06-01

    The operation mechanism of self-correction coil is extended with a simple model. For the ideal self-correction coil case, The self-inductance L of the self-correction coil is calculated. This calculation method is extended to a non-ideal self-correction coil case. For measure of completeness of self-correction coil is measured by the ratio of induced magnetic field by the self-correction coil and error field. Examples are L, M and N calculated for two cases; one is a single block approximation of self-correction coil winding and the other is a two block approximation case. By choosing the adequate angles of self-correction coil winding, one can get about 98% efficiency for single block approximation case and 99.8% for two block approximation case.

  4. Transport Vesicle Tethering at the Trans Golgi Network: Coiled Coil Proteins in Action

    PubMed Central

    Cheung, Pak-yan P.; Pfeffer, Suzanne R.

    2016-01-01

    The Golgi complex is decorated with so-called Golgin proteins that share a common feature: a large proportion of their amino acid sequences are predicted to form coiled-coil structures. The possible presence of extensive coiled coils implies that these proteins are highly elongated molecules that can extend a significant distance from the Golgi surface. This property would help them to capture or trap inbound transport vesicles and to tether Golgi mini-stacks together. This review will summarize our current understanding of coiled coil tethers that are needed for the receipt of transport vesicles at the trans Golgi network (TGN). How do long tethering proteins actually catch vesicles? Golgi-associated, coiled coil tethers contain numerous binding sites for small GTPases, SNARE proteins, and vesicle coat proteins. How are these interactions coordinated and are any or all of them important for the tethering process? Progress toward understanding these questions and remaining, unresolved mysteries will be discussed. PMID:27014693

  5. pH sensitive coiled coils: a strategy for enhanced liposomal drug delivery.

    PubMed

    Reja, Rahi M; Khan, Mohsina; Singh, Sumeet K; Misra, Rajkumar; Shiras, Anjali; Gopi, Hosahudya N

    2016-03-01

    Stimuli responsive controlled release from liposome based vesicles is a promising strategy for the site specific delivery of drugs. Herein, we report the design of pH sensitive coiled coils and their incorporation into the liposome as triggers for the controlled release of encapsulated drugs. The designed coiled coil peptides with the incorporation of environment sensitive fluorescent amino acids were found to be stable at physiological pH and unstructured while changing the pH of the environment to either acidic or basic. This pH dependent conformational switch of the coiled-coil polypeptides was exploited as triggers for the enhanced release of the encapsulated drug molecules from liposomes. The SEM, DLS and TEM analysis revealed the uniform morphology of the peptide liposome hybrid vesicles. Further, the drug encapsulated liposome internalization experiments with cancer cells revealed the enhanced release and accumulation of drugs in the acidic lysosomal compartments in comparison with liposomes without coiled coils. PMID:26876788

  6. Compact stellarators with modular coils

    PubMed Central

    Garabedian, P. R.

    2000-01-01

    Compact stellarator designs with modular coils and only two or three field periods are now available; these designs have both good stability and quasiaxial symmetry providing adequate transport for a magnetic fusion reactor. If the bootstrap current assumes theoretically predicted values a three field period configuration is optimal, but if that net current turns out to be lower, a device with two periods and just 12 modular coils might be better. There are also attractive designs with quasihelical symmetry and four or five periods whose properties depend less on the bootstrap current. Good performance requires that there be a satisfactory magnetic well in the vacuum field, which is a property lacking in a stellarator-tokamak hybrid that has been proposed for a proof of principle experiment. In this paper, we present an analysis of stability for these configurations that is based on a mountain pass theorem asserting that, if two solutions of the problem of magnetohydrodynamic equilibrium can be found, then there has to be an unstable solution. We compare results of our theory of equilibrium, stability, and transport with recently announced measurements from the large LHD experiment in Japan. PMID:10899993

  7. A Non-perturbing Probe of Coiled Coil Formation Based on Electron Transfer Mediated Fluorescence Quenching.

    PubMed

    Watson, Matthew D; Peran, Ivan; Raleigh, Daniel P

    2016-07-01

    Coiled coils are abundant in nature, occurring in ∼3% of proteins across sequenced genomes, and are found in proteins ranging from transcription factors to structural proteins. The motif continues to be an important model system for understanding protein-protein interactions and is finding increased use in bioinspired materials and synthetic biology. Knowledge of the thermodynamics of self-assembly, particularly the dissociation constant KD, is essential for the application of designed coiled coils and for understanding the in vivo specificity of natural coiled coils. Standard methods for measuring KD typically rely on concentration dependent circular dichroism (CD). Fluorescence methods are an attractive alternative; however Trp is rarely found in an interior position of a coiled coil, and appending unnatural fluorophores can perturb the system. We demonstrate a simple, non-perturbing method to monitor coiled coil formation using p-cyanophenylalanine (FCN) and selenomethionine (MSe), the Se analogue of Met. FCN fluorescence can be selectively excited and is effectively quenched by electron transfer with MSe. Both FCN and MSe represent minimally perturbing substitutions in coiled coils. MSe quenching of FCN fluorescence is shown to offer a non-perturbing method for following coiled coil formation and for accurately determining dissociation constants. The method is validated using a designed heterodimeric coiled coil. The KD deduced by fluorescence monitored titration is in excellent agreement with the value deduced from concentration dependent CD measurements to within the uncertainty of the measurement. However, the fluorescence approach requires less protein, is less time-consuming, can be applied to lower concentrations and could be applied to high throughput screens. PMID:27258904

  8. Structural Characteristics of the Redox-sensing Coiled Coil in the Voltage-gated H+ Channel*

    PubMed Central

    Fujiwara, Yuichiro; Takeshita, Kohei; Nakagawa, Atsushi; Okamura, Yasushi

    2013-01-01

    Oxidation is an important biochemical defense mechanism, but it also elicits toxicity; therefore, oxidation must be under strict control. In phagocytotic events in neutrophils, the voltage-gated H+ (Hv) channel is a key regulator of the production of reactive oxygen species against invading bacteria. The cytoplasmic domain of the Hv channel forms a dimeric coiled coil underpinning a dimerized functional unit. Importantly, in the alignment of the coiled-coil core, a conserved cysteine residue forms a potential intersubunit disulfide bond. In this study, we solved the crystal structures of the coiled-coil domain in reduced, oxidized, and mutated (Cys → Ser) states. The crystal structures indicate that a pair of Cys residues forms an intersubunit disulfide bond dependent on the redox conditions. CD spectroscopy revealed that the disulfide bond increases the thermal stability of the coiled-coil protein. We also reveal that two thiol modifier molecules are able to bind to Cys in a redox-dependent manner without disruption of the dimeric coiled-coil assembly. Thus, the biochemical properties of the cytoplasmic coiled-coil domain in the Hv channel depend on the redox condition, which may play a role in redox sensing in the phagosome. PMID:23667254

  9. Accommodation of structural rearrangements in the huntingtin-interacting protein 1 coiled-coil domain

    SciTech Connect

    Wilbur, Jeremy D.; Hwang, Peter K.; Brodsky, Frances M.; Fletterick, Robert J.

    2010-03-01

    Variable packing interaction related to the conformational flexibility within the huntingtin-interacting protein 1 coiled coil domain. Huntingtin-interacting protein 1 (HIP1) is an important link between the actin cytoskeleton and clathrin-mediated endocytosis machinery. HIP1 has also been implicated in the pathogenesis of Huntington’s disease. The binding of HIP1 to actin is regulated through an interaction with clathrin light chain. Clathrin light chain binds to a flexible coiled-coil domain in HIP1 and induces a compact state that is refractory to actin binding. To understand the mechanism of this conformational regulation, a high-resolution crystal structure of a stable fragment from the HIP1 coiled-coil domain was determined. The flexibility of the HIP1 coiled-coil region was evident from its variation from a previously determined structure of a similar region. A hydrogen-bond network and changes in coiled-coil monomer interaction suggest that the HIP1 coiled-coil domain is uniquely suited to allow conformational flexibility.

  10. Natural templates for coiled-coil biomaterials from praying mantis egg cases.

    PubMed

    Walker, Andrew A; Weisman, Sarah; Kameda, Tsunenori; Sutherland, Tara D

    2012-12-10

    Whereas there is growing interest in producing biomaterials containing coiled-coils, relatively few studies have made use of naturally occurring fibrous proteins. In this study, we have characterized fibrous proteins used by mother praying mantises to produce an extensive covering for their eggs called an ootheca and demonstrate the production of artificial ootheca using recombinantly produced proteins. Examination of natural oothecae by infrared spectroscopy and solid-state nuclear magnetic resonance revealed the material to consist of proteins organized predominately as coiled-coils. Two structural proteins, Mantis Fibroin 1 and Mantis Fibroin 2, were identified in ootheca from each of three species. Between species, the primary sequences of both proteins had diverged considerably, but other features were tightly conserved, including low molecular weight, high abundance of Ala, Glu, Lys, and Ser, and a triblock-like architecture with extensive central coiled-coil domain. Mantis fibroin hydrophobic cores had an unusual composition containing high levels of alanine and aromatic residues. Recombinantly produced mantis fibroins folded into coiled-coils in solution and could be fabricated into solid materials with high coiled-coil content. The structural features of mantis fibroins and their straightforward recombinant production make them promising templates for the production of coiled-coil biomimetics materials. PMID:23137042

  11. A comparative study of flat coil and coil sensor for landslide detection

    NASA Astrophysics Data System (ADS)

    Sanjaya, Edi; Muslimin, Ahmad Novi; Djamal, Mitra; Suprijadi, Handayani, Gunawan; Ramli

    2016-03-01

    The landslide is one of the most costly catastrophic events in terms of human lives and infrastructure damage, thus an early warning monitoring for landslides becomes more and more important. Currently existing monitoring systems for early warning are available in terms of monolithic systems. This is a very cost-intensive way, considering installation as well as operational and personal expenses. We have been developing a landslide detection system based on flat coil and coil sensor. The flat coil element being developed is an inductive proximity sensor for detection mass of soil movement. The simple method of flat coil manufactures and low cost, is an attraction that is still inspired to develop flat coil sensors. Meanwhile, although it has a drawback in terms of their size, the coil sensor is still required in many fields due to their sensitivity and robustness. The simple method of coil manufacture and the materials are commonly available and low cost, is an attraction that is still inspired to develop induction coil sensors. A comparative study of alternative configuration of sensor based on flat coil elements and a coil in application to landslide detection has been discussed in this paper. The purpose of this comparison is to show the ideal conditions and the challenges for each sensor. Furthermore, a comparison between flat coil and coil sensor is presented.

  12. Designed Coiled-Coil Peptides Inhibit the Type Three Secretion System of Enteropathogenic Escherichia coli

    PubMed Central

    Larzábal, Mariano; Mercado, Elsa C.; Vilte, Daniel A.; Salazar-González, Hector; Cataldi, Angel; Navarro-Garcia, Fernando

    2010-01-01

    Background Enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are two categories of E. coli strains associated with human disease. A major virulence factor of both pathotypes is the expression of a type three secretion system (TTSS), responsible for their ability to adhere to gut mucosa causing a characteristic attaching and effacing lesion (A/E). The TTSS translocates effector proteins directly into the host cell that subvert mammalian cell biochemistry. Methods/Principal Findings We examined synthetic peptides designed to inhibit the TTSS. CoilA and CoilB peptides, both representing coiled-coil regions of the translocator protein EspA, and CoilD peptide, corresponding to a coiled–coil region of the needle protein EscF, were effective in inhibiting the TTSS dependent hemolysis of red blood cells by the EPEC E2348/69 strain. CoilA and CoilB peptides also reduced the formation of actin pedestals by the same strain in HEp-2 cells and impaired the TTSS-mediated protein translocation into the epithelial cell. Interestingly, CoilA and CoilB were able to block EspA assembly, destabilizing the TTSS and thereby Tir translocation. This blockage of EspA polymerization by CoilA or CoilB peptides, also inhibited the correct delivery of EspB and EspD as detected by immunoblotting. Interestingly, electron microscopy of bacteria incubated with the CoilA peptide showed a reduction of the length of EspA filaments. Conclusions Our data indicate that coiled-coil peptides can prevent the assembly and thus the functionality of the TTSS apparatus and suggest that these peptides could provide an attractive tool to block EPEC and EHEC pathogenesis. PMID:20140230

  13. Undulator Long Coil Measurement System Tests

    SciTech Connect

    Wolf, Zachary; Levashov, Yurii; /SLAC

    2010-11-24

    The first and second field integrals in the LCLS undulators must be below a specified limit. To accurately measure the field integrals, a long coil system is used. This note describes a set of tests which were used to check the performance of the long coil system. A long coil system was constructed to measure the first and second field integrals of the LCLS undulators. The long coil measurements of the background fields were compared to field integrals obtained by sampling the background fields and numerically calculating the integrals. This test showed that the long coil has the sensitivity required to measure at the levels specified for the field integrals. Tests were also performed by making long coil measurements of short magnets of known strength placed at various positions The long coil measurements agreed with the known field integrals obtained by independent measurements and calculation. Our tests showed that the long coil measurements are a valid way to determine whether the LCLS undulator field integrals are below the specified limits.

  14. Magnetic Fields at the Center of Coils

    ERIC Educational Resources Information Center

    Binder, Philippe; Hui, Kaleonui; Goldman, Jesse

    2014-01-01

    In this note we synthesize and extend expressions for the magnetic field at the center of very short and very long current-carrying coils. Elementary physics textbooks present the following equation for the magnetic field inside a very long current-carrying coil (solenoid): B[subscript sol] = µ[subscript 0] (N/L) I, (1) where I is the current, N…

  15. Precise Fabrication of Electromagnetic-Levitation Coils

    NASA Technical Reports Server (NTRS)

    Ethridge, E.; Curreri, P.; Theiss, J.; Abbaschian, G.

    1985-01-01

    Winding copper tubing on jig ensures reproducible performance. Sequence of steps insures consistent fabrication of levitation-and-melting coils. New method enables technician to produce eight coils per day, 95 percent of them acceptable. Method employs precise step-by-step procedure on specially designed wrapping and winding jig.

  16. Novel transcranial magnetic stimulation coil for mice

    NASA Astrophysics Data System (ADS)

    March, Stephen; Stark, Spencer; Crowther, Lawrence; Hadimani, Ravi; Jiles, David

    2014-03-01

    Transcranial magnetic stimulation (TMS) shows potential for non-invasive treatment of various neurological disorders. Significant work has been performed on the design of coils used for TMS on human subjects but few reports have been made on the design of coils for use on the brains of animals such as mice. This work is needed as TMS studies utilizing mice can allow rapid preclinical development of TMS for human disorders but the coil designs developed for use on humans are inadequate for optimal stimulation of the much smaller mouse brain. A novel TMS coil has been developed with the goal of inducing strong and focused electric fields for the stimulation of small animals such as mice. Calculations of induced electric fields were performed utilizing an MRI derived inhomogeneous model of an adult male mouse. Mechanical and thermal analysis of this new TMS helmet-coil design have also been performed at anticipated TMS operating conditions to ensure mechanical stability of the new coil and establish expected linear attraction and rotational force values. Calculated temperature increases for typical stimulation periods indicate the helmet-coil system is capable of operating within established medical standards. A prototype of the coil has been fabricated and characterization results are presented.

  17. Optimal Coil Orientation for Transcranial Magnetic Stimulation

    PubMed Central

    Richter, Lars; Neumann, Gunnar; Oung, Stephen; Schweikard, Achim; Trillenberg, Peter

    2013-01-01

    We study the impact of coil orientation on the motor threshold (MT) and present an optimal coil orientation for stimulation of the foot. The result can be compared to results of models that predict this orientation from electrodynamic properties of the media in the skull and from orientations of cells, respectively. We used a robotized TMS system for precise coil placement and recorded motor-evoked potentials with surface electrodes on the abductor hallucis muscle of the right foot in 8 healthy control subjects. First, we performed a hot-spot search in standard (lateral) orientation and then rotated the coil in steps of 10° or 20°. At each step we estimated the MT. For navigated stimulation and for correlation with the underlying anatomy a structural MRI scan was obtained. Optimal coil orientation was 33.1±18.3° anteriorly in relation to the standard lateral orientation. In this orientation the threshold was 54±18% in units of maximum stimulator output. There was a significant difference of 8.0±5.9% between the MTs at optimal and at standard orientation. The optimal coil orientations were significantly correlated with the direction perpendicular to the postcentral gyrus (). Robotized TMS facilitates sufficiently precise coil positioning and orientation to study even small variations of the MT with coil orientation. The deviations from standard orientation are more closely matched by models based on field propagation in media than by models based on orientations of pyramidal cells. PMID:23593200

  18. Completion of the Polo model coil

    SciTech Connect

    Bourquard, A.; Plat, X.; Bonnet, P.; Semal, D.; Personeni, G.; Bernaudat, M.; Hacquard, A.; Salvador, R.; Dombrowski, D.

    1996-07-01

    A superconducting poloidal field model coil as needed for tokamaks has been constructed by GEC Alsthom within the Polo project in effective collaboration with Forschungszentrum Karlsruhe, Institut fuer Technische Physik, Germany. The manufacturing procedures for the coil and its terminals are described.

  19. Operator coil monitoring Acceptance Test Procedure

    SciTech Connect

    Erhart, M.F.

    1995-05-16

    The readiness of the Data Acquisition and Control System (DACS) to provide monitoring and control of the Programmable Logic Controller (PLC) abort coils from the Master and RSS stations will be systematically tested during performance of this procedure. It should be noted that these are not physical abort coils but software coils controlled by the software`s ladder logic. The readiness of the DACS to properly interface with the ENRAF wire level gauge installed in the SY-101 storage tank will also be tested. During this test, a verification of all abort coil indications will be conducted at the DACS Development Facility in the 306E Building by injecting an input signal for each DACS sensor that has an associated abort coil until the abort coil actuates, and then ensuring that the status of the abort coil indicated at the Master and RSS stations is correct. Each abort coil will also be tested to ensure that the ``ENABLE`` and ``DISABLE`` controls from the Master and RSS stations function correctly, and only with the use of proper passwords.

  20. Operator coil monitoring acceptance test procedure

    SciTech Connect

    Erhart, M.F.

    1995-06-05

    The readiness of the Data Acquisition and Control System (DACS) to provide monitoring and control of the Programmable Logic Controller (PLC) abort coils from the Master and RSS stations will be systematically tested during performance of this procedure. It should be noted that these are not physical abort coils but software coils controlled by the software`s ladder logic. The readiness of the DACS to properly interface with the ENRAF wire level gauge installed in the SY101 storage tank will also be tested. During this test, a verification of all abort coil indications will be conducted at the DACS Development Facility in the 306E Building by injecting an input signal for each DACS sensor that has an associated abort coil until the abort coil actuates, and then ensuring that the status of the abort coil indicated at the Master and RSS stations correct. Each abort coil will also be tested to ensure that the ``ENABLE`` and ``DISABLE`` controls from the Master and RSS stations function correctly, and only with the use of proper passwords.

  1. Helical axis stellarator with noninterlocking planar coils

    DOEpatents

    Reiman, A.; Boozer, A.H.

    1984-03-06

    The present invention generates stellarator fields having favorable properties (magnetic well and large rotational transform) by a simple coil system consisting only of unlinked planar non-circular coils. At large rotational transform toroidal effects on magnetic well and rotational transform are small and can be ignored. We do so herein, specializing in straight helical systems.

  2. Rod-Coil Block Polyimide Copolymers

    NASA Technical Reports Server (NTRS)

    Meador, Mary Ann B. (Inventor); Kinder, James D. (Inventor)

    2005-01-01

    This invention is a series of rod-coil block polyimide copolymers that are easy to fabricate into mechanically resilient films with acceptable ionic or protonic conductivity at a variety of temperatures. The copolymers consist of short-rigid polyimide rod segments alternating with polyether coil segments. The rods and coil segments can be linear, branched or mixtures of linear and branched segments. The highly incompatible rods and coil segments phase separate, providing nanoscale channels for ion conduction. The polyimide segments provide dimensional and mechanical stability and can be functionalized in a number of ways to provide specialized functions for a given application. These rod-coil black polyimide copolymers are particularly useful in the preparation of ion conductive membranes for use in the manufacture of fuel cells and lithium based polymer batteries.

  3. MIT 12 Tesla Coil test results

    NASA Astrophysics Data System (ADS)

    Steeves, M. M.; Hoenig, M. O.

    1985-07-01

    Test results from the MIT 12 Tesla Coil experiment are presented. The coil was tested in the High Field Test Facility (HFTF) of the Lawrence Livermore National Laboratory in October 1984 and January 1985. The experiment measured the performance of an Internally Cooled, Cabled Superconductor (ICCS) of practical size, intended for use in magnetic fusion experiments. The MIT coil carried 15 kA at 11 T for 5 min with no sign of instability. A half turn length in a 10 T field was able to absorb a heat load in 4 msec of more than 200 mJ sub cm of cable volume while carrying a current of 12 kA. The MIT coil successfully met the performance requirements of the Department of Energy's 12 Tesla Coil Program.

  4. Recent advances in helix-coil theory.

    PubMed

    Doig, Andrew J

    2002-12-10

    Peptide helices in solution form a complex mixture of all helix, all coil or, most frequently, central helices with frayed coil ends. In order to interpret experiments on helical peptides and make theoretical predictions on helices, it is therefore essential to use a helix-coil theory that takes account of this equilibrium. The original Zimm-Bragg and Lifson-Roig helix-coil theories have been greatly extended in the last 10 years to include additional interactions. These include preferences for the N-cap, N1, N2, N3 and C-cap positions, capping motifs, helix dipoles, side chain interactions and 3(10)-helix formation. These have been applied to determine energies for these preferences from experimental data and to predict the helix contents of peptides. This review discusses these newly recognised structural features of helices and how they have been included in helix-coil models. PMID:12488008

  5. NMR local coil with adjustable spacing

    SciTech Connect

    Dembinski, G.T.

    1988-03-22

    A local coil assembly for use in NMR imaging is described which comprises: a base; a first local coil module mounted to the base and extending upward therefrom; sockets disposed in the base, each at a different distance from the first local coil module; a second local coil module having a connector therein which mates with each of the sockets to enable the second local coil module to be connected to the base at any one of the sockets; and a set of reactive components. The values of the respective reactive components are selected such that the second local oil module may be connected to any of the sockets without any substantial change in the resonant frequency of the assembly.

  6. Intra-coil interactions in split gradient coils in a hybrid MRI-LINAC system

    NASA Astrophysics Data System (ADS)

    Tang, Fangfang; Freschi, Fabio; Sanchez Lopez, Hector; Repetto, Maurizio; Liu, Feng; Crozier, Stuart

    2016-04-01

    An MRI-LINAC system combines a magnetic resonance imaging (MRI) system with a medical linear accelerator (LINAC) to provide image-guided radiotherapy for targeting tumors in real-time. In an MRI-LINAC system, a set of split gradient coils is employed to produce orthogonal gradient fields for spatial signal encoding. Owing to this unconventional gradient configuration, eddy currents induced by switching gradient coils on and off may be of particular concern. It is expected that strong intra-coil interactions in the set will be present due to the constrained return paths, leading to potential degradation of the gradient field linearity and image distortion. In this study, a series of gradient coils with different track widths have been designed and analyzed to investigate the electromagnetic interactions between coils in a split gradient set. A driving current, with frequencies from 100 Hz to 10 kHz, was applied to study the inductive coupling effects with respect to conductor geometry and operating frequency. It was found that the eddy currents induced in the un-energized coils (hereby-referred to as passive coils) positively correlated with track width and frequency. The magnetic field induced by the eddy currents in the passive coils with wide tracks was several times larger than that induced by eddy currents in the cold shield of cryostat. The power loss in the passive coils increased with the track width. Therefore, intra-coil interactions should be included in the coil design and analysis process.

  7. A study on geometry effect of transmission coil for micro size magnetic induction coil

    NASA Astrophysics Data System (ADS)

    Lee, Kyung Hwa; Jun, Byoung Ok; Kim, Seunguk; Lee, Gwang Jun; Ryu, Mingyu; Choi, Ji-Woong; Jang, Jae Eun

    2016-05-01

    The effects of transmission (Tx) coil structure have been studied for micro-size magnetic induction coil. The size of the receiving (Rx) coil should be shrunk to the micrometer level for the various new applications such as micro-robot and wireless body implanted devices. In case of the macro-scale magnetic induction coil, the power transmission efficiency is generally considered to be higher as the inductance of the transmission coil became larger; however, the large size difference between macro-size Tx coil and micro-size Rx coil can decrease the power transmission efficiency due to the difference of resonance frequency. Here, we study a correlation of the power transmission with the size and distance between the macro-size Tx and micro-size Rx coils using magnetic induction technique. The maximum power efficiency was 0.28/0.23/0.13/0.12% at the distance of 0.3/1/3/5 cm between Rx and Tx coil. In addition, more efficient wireless power transferring method is suggested with a floating coil for the body implantable devices. The voltage output increased up to 5.4 mV than the original one Tx coil system. The results demonstrated the foundational wireless power transferring system with enhanced power efficiency.

  8. Intra-coil interactions in split gradient coils in a hybrid MRI-LINAC system.

    PubMed

    Tang, Fangfang; Freschi, Fabio; Sanchez Lopez, Hector; Repetto, Maurizio; Liu, Feng; Crozier, Stuart

    2016-04-01

    An MRI-LINAC system combines a magnetic resonance imaging (MRI) system with a medical linear accelerator (LINAC) to provide image-guided radiotherapy for targeting tumors in real-time. In an MRI-LINAC system, a set of split gradient coils is employed to produce orthogonal gradient fields for spatial signal encoding. Owing to this unconventional gradient configuration, eddy currents induced by switching gradient coils on and off may be of particular concern. It is expected that strong intra-coil interactions in the set will be present due to the constrained return paths, leading to potential degradation of the gradient field linearity and image distortion. In this study, a series of gradient coils with different track widths have been designed and analyzed to investigate the electromagnetic interactions between coils in a split gradient set. A driving current, with frequencies from 100Hz to 10kHz, was applied to study the inductive coupling effects with respect to conductor geometry and operating frequency. It was found that the eddy currents induced in the un-energized coils (hereby-referred to as passive coils) positively correlated with track width and frequency. The magnetic field induced by the eddy currents in the passive coils with wide tracks was several times larger than that induced by eddy currents in the cold shield of cryostat. The power loss in the passive coils increased with the track width. Therefore, intra-coil interactions should be included in the coil design and analysis process. PMID:26852418

  9. Performance improvement of a high-temperature superconducting coil by separating and grading the coil edge

    NASA Astrophysics Data System (ADS)

    Ishiguri, Shinichi; Funamoto, Taisuke

    2011-06-01

    In this paper, we establish a model to analyze the transport current performance of a high-temperature superconducting (HTS) coil, considering the dependencies of critical current and n-value of an HTS tape on magnetic field and magnetic field angles. This analysis shows that relatively large electric fields appear at the coil’s edges, preventing improvement in the transport current performance of the coil. To solve this problem, in this paper, we propose a graded coil in which several coil edges of different heights are separated and graded. Analysis of its performance shows that the coil’s critical current increases, thus confirming that there exists an optimum coil cross section at which the stored energy and central magnetic field improve 2.1 times and 45%, respectively, compared with a typical rectangular coil that employs the same total length of the HTS tape. It is recommended that these results of the coil should be applied to SMES.

  10. Optimization of RMP Coils for ELM Control

    NASA Astrophysics Data System (ADS)

    Dutta, Someswar; Evans, T. E.; Orlov, D. M.

    2015-11-01

    Advanced DIII-D RMP coils with improved capabilities are studied using a vacuum island overlap width (VIOW) criterion. Changes in characteristics of the RMP field produced by different geometrical parameters using both ex-vessel (C- and O-) and in-vessel (I- and CP-) coils are discussed. By reducing the poloidal span of each coil, the spacing between them and varying the geometric angle between the coils and the plasma, the resonant field can be adjusted to optimize the edge VIOW criterion while minimizing core resonances. Three separate phase scans using a combination of the as built I-coils and proposed CP-coils are compared for three different equilibria. Two of these equilibria have different edge safety factors and the third one has a different gap between plasma and wall than the standard equilibrium scenario of DIII D. The scan results show that the VIOW correlation criterion is well satisfied in all three cases, resulting in a new way to optimize the RMP coils for the future reactors in order to achieve the ELM suppression criterion over a significantly wider range of fusion plasma operating scenarios. Work supported by the U.S. DOE under DE-FG02-05ER54809 and DE-FC02-04ER54698.

  11. ENGINEERING OF THE AGS SNAKE COIL ASSEMBLY.

    SciTech Connect

    ANERELLA,M.GUPTA,R.KOVACH,P.MARONE,A.PLATE,S.POWER,K.SCHMALZLE,J.WILLEN,E.

    2003-05-12

    A 30% Snake superconducting magnet is proposed to maintain polarization in the AGS proton beam, the magnetic design of which is described elsewhere. The required helical coils for this magnet push the limits of the technology developed for the RHIC Snake coils. First, fields must be provided with differing pitch along the length of the magnet. To accomplish this, a new 3-D CAD system (''Pro/Engineer'' from PTC), which uses parametric techniques to enable fast iterations, has been employed. Revised magnetic field calculations are then based on the output of the mechanical model. Changes are made in turn to the model on the basis of those field calculations. To ensure that accuracy is maintained, the final solid model is imported directly into the CNC machine programming software, rather than by the use of graphics translating software. Next, due to the large coil size and magnetic field, there was concern whether the structure could contain the coil forces. A finite element analysis was performed, using the 3-D model, to ensure that the stresses and deflections were acceptable. Finally, a method was developed using ultrasonic energy to improve conductor placement during coil winding, in an effort to minimize electrical shorts due to conductor misplacement, a problem that occurred in the RHIC helical coil program. Each of these activities represents a significant improvement in technology over that which was used previously for the RHIC snake coils.

  12. Switching transients in a superconducting coil

    SciTech Connect

    Owen, E.W.; Shimer, D.W.

    1983-11-18

    A study is made of the transients caused by the fast dump of large superconducting coils. Theoretical analysis, computer simulation, and actual measurements are used. Theoretical analysis can only be applied to the simplest of models. In the computer simulations two models are used, one in which the coil is divided into ten segments and another in which a single coil is employed. The circuit breaker that interrupts the current to the power supply, causing a fast dump, is represented by a time and current dependent conductance. Actual measurements are limited to measurements made incidental to performance tests on the MFTF Yin-yang coils. It is found that the breaker opening time is the critical factor in determining the size and shape of the transient. Instantaneous opening of the breaker causes a lightly damped transient with large amplitude voltages to ground. Increasing the opening time causes the transient to become a monopulse of decreasing amplitude. The voltages at the external terminals are determined by the parameters of the external circuit. For fast opening times the frequency depends on the dump resistor inductance, the circuit capacitance, and the amplitude on the coil current. For slower openings the dump resistor inductance and the current determine the amplitude of the voltage to ground at the terminals. Voltages to ground are less in the interior of the coil, where transients related to the parameters of the coil itself are observed.

  13. Microsensor coils for miniature fiber optic gyroscopes

    NASA Astrophysics Data System (ADS)

    Ruffin, Paul B.; Baeder, Janet S.

    2004-10-01

    Depolarized Interferometric Fiber Optic Gyroscopes (D-IFOGs) that are constructed with inexpensive single mode (SM) fiber have provided an opportunity for developers to meet Army emerging missions goals for affordable, small volume, reliable inertial guidance systems for use in small missiles, munitions, and future micro-unmanned autonomous vehicles. However, there remain several vital issues associated with substantially reducing the diameter of the sensor coil. Optical fiber that is precision-wound onto a micro coil experiences increased stress due to small radius bending, fiber distortions at crossover sites, and increased interlayer pressures as a result of multiple layers of fiber wound under tension. Tension and small radius bending stresses can have a detrimental effect on the performance of D-IFOGs. Therefore, other scenarios for the application of SM fiber to a micro-sensor coil must be considered. One scheme involves taking advantage of the bending-induced birefringence and employing the low cost SM fiber as a polarization-maintaining (PM) fiber. The mechanics of how a substantial reduction in the coil radius produces PM fiber properties in SM fiber is investigated under this research effort. Conventional and specialty SM fibers are characterized to identify optimal fibers for the development of micro-sensor coils. The results from extinction ratio measurements on the SM fibers and micro-sensor coils are presented in this paper. The significant cross coupling suggests that scattering centers are present in very small radius bending. Also, measurements show that optical loss is significant in micro IFOG coils.

  14. Phase reconstruction from multiple coil data using a virtual reference coil

    PubMed Central

    Parker, Dennis L.; Payne, Allison; Todd, Nick; Hadley, J. Rock

    2013-01-01

    Purpose This paper develops a method to obtain optimal estimates of absolute magnetization phase from multiple-coil MRI data. Methods The element-specific phases of a multi-element receiver coil array are accounted for by using the phase of a real or virtual reference coil that is sensitive over the entire imaged volume. The virtual-reference coil is generated as a weighted combination of measurements from all receiver coils. The phase-corrected multiple coil complex images are combined using the inverse covariance matrix. These methods are tested on images of an agar phantom, an in vivo breast, and an anesthetized rabbit obtained using combinations of four, nine, and three receiver channels, respectively. Results The four- and three- channel acquisitions require formation of a virtual-reference receiver coil while one channel of the nine-channel receive array has a sensitivity profile covering the entire imaged volume. Referencing to a real or virtual coil gives receiver phases that are essentially identical except for the individual receiver channel noise. The resulting combined images, which account for receiver channel noise covariance, show the expected reduction in phase variance. Conclusions The proposed virtual reference coil method determines a phase distribution for each coil from which an optimal phase map can be obtained. PMID:24006172

  15. Crystal Structure of the Central Coiled-Coil Domain from Human Liprin-[beta]2

    SciTech Connect

    Stafford, Ryan L.; Tang, Ming-Yun; Sawaya, Michael R.; Phillips, Martin L.; Bowie, James U.

    2012-02-07

    Liprins are a conserved family of scaffolding proteins important for the proper regulation and development of neuronal synapses. Humans have four liprin-{alpha}s and two liprin-{beta}s which all contain long coiled-coil domains followed by three tandem SAM domains. Complex interactions between the coiled-coil and SAM domains are thought to create liprin scaffolds, but the structural and biochemical properties of these domains remain largely uncharacterized. In this study we find that the human liprin-{beta}2 coiled-coil forms an extended dimer. Several protease-resistant subdomains within the liprin-{beta}1 and liprin-{beta}2 coiled-coils were also identified. A 2.0 {angstrom} crystal structure of the central, protease-resistant core of the liprin-{beta}2 coiled-coil reveals a parallel helix orientation. These studies represent an initial step toward determining the overall architecture of liprin scaffolds and understanding the molecular basis for their synaptic functions.

  16. Crystal Structure of a Coiled-Coil Domain from Human ROCK I

    PubMed Central

    Tu, Daqi; Li, Yiqun; Song, Hyun Kyu; Toms, Angela V.; Gould, Christopher J.; Ficarro, Scott B.; Marto, Jarrod A.; Goode, Bruce L.; Eck, Michael J.

    2011-01-01

    The small GTPase Rho and one of its targets, Rho-associated kinase (ROCK), participate in a variety of actin-based cellular processes including smooth muscle contraction, cell migration, and stress fiber formation. The ROCK protein consists of an N-terminal kinase domain, a central coiled-coil domain containing a Rho binding site, and a C-terminal pleckstrin homology domain. Here we present the crystal structure of a large section of the central coiled-coil domain of human ROCK I (amino acids 535–700). The structure forms a parallel α-helical coiled-coil dimer that is structurally similar to tropomyosin, an actin filament binding protein. There is an unusual discontinuity in the coiled-coil; three charged residues (E613, R617 and D620) are positioned at what is normally the hydrophobic core of coiled-coil packing. We speculate that this conserved irregularity could function as a hinge that allows ROCK to adopt its autoinhibited conformation. PMID:21445309

  17. A Synthetic Coiled-Coil Interactome Provides Heterospecific Modules for Molecular Engineering

    SciTech Connect

    Reinke, Aaron W.; Grant, Robert A.; Keating, Amy E.

    2010-06-21

    The versatile coiled-coil protein motif is widely used to induce and control macromolecular interactions in biology and materials science. Yet the types of interaction patterns that can be constructed using known coiled coils are limited. Here we greatly expand the coiled-coil toolkit by measuring the complete pairwise interactions of 48 synthetic coiled coils and 7 human bZIP coiled coils using peptide microarrays. The resulting 55-member protein 'interactome' includes 27 pairs of interacting peptides that preferentially heteroassociate. The 27 pairs can be used in combinations to assemble sets of 3 to 6 proteins that compose networks of varying topologies. Of special interest are heterospecific peptide pairs that participate in mutually orthogonal interactions. Such pairs provide the opportunity to dimerize two separate molecular systems without undesired crosstalk. Solution and structural characterization of two such sets of orthogonal heterodimers provide details of their interaction geometries. The orthogonal pair, along with the many other network motifs discovered in our screen, provide new capabilities for synthetic biology and other applications.

  18. Comparison of Coil and Kiil Dialysers

    PubMed Central

    Down, P. F.; Farrand, D. E.; Wood, S. E.; Lee, H. A.

    1970-01-01

    To assess the comparative efficiency, safety, and cost of maintenance dialysis, the treatment of 13 patients with a Kiil dialyser (representing 1,477 hospital and 735 home dialyses) was compared with that of 11 patients using a coil dialyser (898 hospital and 396 home dialyses). Kiil and coil dialysers proved equally satisfactory from a medical standpoint and equally acceptable to the patients. The capital costs of home dialysis were considerably reduced without any threat to safety or efficiency. The running costs of coil dialysers approximate to those of Kiil dialysers. ImagesFig. 3 PMID:4320676

  19. Coiled Fiber Pulsed Laser Simulator

    Energy Science and Technology Software Center (ESTSC)

    2009-01-29

    This suite of codes simulates the transient output pulse from an optically-pumped coiled fiber amplifier. The input pulse is assumed to have a Gaussian time dependence and a spatial dependence that may be Gaussian or an eigenmode of the straight of bent fiber computed using bend10 or bend20. Only one field component is used (semivectorial approximation). The fully-spatially-dependent fiber gain profile is specified is subroutines "inversion" and "interp_inversion" and is presently read from a datamore » file, although other means of specifying fiber gain could be reallized through modification of these subroutines. The input pulse is propagated through the fiber, including the following physical effects: spatial and temporal gain saturation, self-focusing, bend losses, and confinement from a user-defined fiber index profile. The user can follow the propagation progress with 3D graphics that show an intensity profile via user-modifiable cutting planes through the time space axes. A restart capability is also included. Approximate solutions in the frequency domain may be obtained much faster using the auxilliary codes bendbpm10 (full vector), bendbpm20 (semivectoral), and bendbpm21 (semivectoral with gain sheet spproximation for gain and self-focusing). These codes all include bend loss and spatial (but not temporal) gain saturation.« less

  20. Helical coil thermal hydraulic model

    NASA Astrophysics Data System (ADS)

    Caramello, M.; Bertani, C.; De Salve, M.; Panella, B.

    2014-11-01

    A model has been developed in Matlab environment for the thermal hydraulic analysis of helical coil and shell steam generators. The model considers the internal flow inside one helix and its associated control volume of water on the external side, both characterized by their inlet thermodynamic conditions and the characteristic geometry data. The model evaluates the behaviour of the thermal-hydraulic parameters of the two fluids, such as temperature, pressure, heat transfer coefficients, flow quality, void fraction and heat flux. The evaluation of the heat transfer coefficients as well as the pressure drops has been performed by means of the most validated literature correlations. The model has been applied to one of the steam generators of the IRIS modular reactor and a comparison has been performed with the RELAP5/Mod.3.3 code applied to an inclined straight pipe that has the same length and the same elevation change between inlet and outlet of the real helix. The predictions of the developed model and RELAP5/Mod.3.3 code are in fairly good agreement before the dryout region, while the dryout front inside the helical pipes is predicted at a lower distance from inlet by the model.

  1. Coiled Fiber Pulsed Laser Simulator

    SciTech Connect

    Hadley, G. Ronald

    2009-01-29

    This suite of codes simulates the transient output pulse from an optically-pumped coiled fiber amplifier. The input pulse is assumed to have a Gaussian time dependence and a spatial dependence that may be Gaussian or an eigenmode of the straight of bent fiber computed using bend10 or bend20. Only one field component is used (semivectorial approximation). The fully-spatially-dependent fiber gain profile is specified is subroutines "inversion" and "interp_inversion" and is presently read from a data file, although other means of specifying fiber gain could be reallized through modification of these subroutines. The input pulse is propagated through the fiber, including the following physical effects: spatial and temporal gain saturation, self-focusing, bend losses, and confinement from a user-defined fiber index profile. The user can follow the propagation progress with 3D graphics that show an intensity profile via user-modifiable cutting planes through the time space axes. A restart capability is also included. Approximate solutions in the frequency domain may be obtained much faster using the auxilliary codes bendbpm10 (full vector), bendbpm20 (semivectoral), and bendbpm21 (semivectoral with gain sheet spproximation for gain and self-focusing). These codes all include bend loss and spatial (but not temporal) gain saturation.

  2. Electrical wire insulation and electromagnetic coil

    DOEpatents

    Bich, George J.; Gupta, Tapan K.

    1984-01-01

    An electromagnetic coil for high temperature and high radiation application in which glass is used to insulate the electrical wire. A process for applying the insulation to the wire is disclosed which results in improved insulation properties.

  3. Screen-printed flexible MRI receive coils

    NASA Astrophysics Data System (ADS)

    Corea, Joseph R.; Flynn, Anita M.; Lechêne, Balthazar; Scott, Greig; Reed, Galen D.; Shin, Peter J.; Lustig, Michael; Arias, Ana C.

    2016-03-01

    Magnetic resonance imaging is an inherently signal-to-noise-starved technique that limits the spatial resolution, diagnostic image quality and results in typically long acquisition times that are prone to motion artefacts. This limitation is exacerbated when receive coils have poor fit due to lack of flexibility or need for padding for patient comfort. Here, we report a new approach that uses printing for fabricating receive coils. Our approach enables highly flexible, extremely lightweight conforming devices. We show that these devices exhibit similar to higher signal-to-noise ratio than conventional ones, in clinical scenarios when coils could be displaced more than 18 mm away from the body. In addition, we provide detailed material properties and components performance analysis. Prototype arrays are incorporated within infant blankets for in vivo studies. This work presents the first fully functional, printed coils for 1.5- and 3-T clinical scanners.

  4. Coiling Temperature Control in Hot Strip Mill

    NASA Astrophysics Data System (ADS)

    Imanari, Hiroyuki; Fujiyama, Hiroaki

    Coiling temperature is one of the most significant factors in products of hot strip mill to determine material properties such as strength, toughness of steel, so it is very important to achieve accurate coiling temperature control (CTC). Usually there are a few pyrometers on the run out table in hot strip mill, therefore temperature model and its adapting system have large influences on the accuracy of CTC. Also unscheduled change of rolling speed has a bad effect to keep coiling temperature as its target. Newly developed CTC system is able to get very accurate coiling temperature against uncertain factors and disturbances by adopting easily identified temperature model, learning method and dynamic set up function. The features of the CTC system are discussed with actual data, and the effectiveness of the system is shown by actual control results.

  5. Electrical Wire Insulation and Electromagnetic Coil

    SciTech Connect

    Bich, G. J.; Gupta, T. K.

    1984-01-31

    An electromagnetic coil for high temperature and high radiation application in which glass is used to insulate the electrical wire. A process for applying the insulation to the wire is disclosed which results in improved insulation properties.

  6. Screen-printed flexible MRI receive coils.

    PubMed

    Corea, Joseph R; Flynn, Anita M; Lechêne, Balthazar; Scott, Greig; Reed, Galen D; Shin, Peter J; Lustig, Michael; Arias, Ana C

    2016-01-01

    Magnetic resonance imaging is an inherently signal-to-noise-starved technique that limits the spatial resolution, diagnostic image quality and results in typically long acquisition times that are prone to motion artefacts. This limitation is exacerbated when receive coils have poor fit due to lack of flexibility or need for padding for patient comfort. Here, we report a new approach that uses printing for fabricating receive coils. Our approach enables highly flexible, extremely lightweight conforming devices. We show that these devices exhibit similar to higher signal-to-noise ratio than conventional ones, in clinical scenarios when coils could be displaced more than 18 mm away from the body. In addition, we provide detailed material properties and components performance analysis. Prototype arrays are incorporated within infant blankets for in vivo studies. This work presents the first fully functional, printed coils for 1.5- and 3-T clinical scanners. PMID:26961073

  7. Magnetic propulsion of a magnetic device using three square-Helmholtz coils and a square-Maxwell coil.

    PubMed

    Ha, Yong H; Han, Byung H; Lee, Soo Y

    2010-02-01

    We introduce a square coil system for remote magnetic navigation of a magnetic device without any physical movements of the coils. We used three square-Helmholtz coils and a square-Maxwell coil for magnetic propulsion of a small magnet along the desired path. All the square coils are mountable on a cubic frame that has an opening to accommodate a living subject. The square-Helmholtz coils control the magnetic propulsion direction by generating uniform magnetic field along the desired direction while the square-Maxwell coil controls the propulsion force by generating magnetic gradient field. We performed magnetic propulsion experiments with a down-scaled coil set and a three-channel coil driver. Experimental results demonstrate that we can use the square coil set for magnetic navigation of a magnetic device without any physical movements of the coils. PMID:20054666

  8. Passive energy dump for superconducting coil protection

    DOEpatents

    Luton, J.N. Jr.

    1973-01-16

    The patent describes a passive resistance type energy dump for the protection of the coils of a superconducting magnet. Insertion heaters are immersed in a rigid container filled with a fusible alloy. The energy dump is connected across the coils of the superconducting magnet wherein individual heater elements are connected singly to the windings or otherwise according to the energy dumping requirements upon transition of the magnet to a normal state.

  9. Heterogeneous Superconducting Low-Noise Sensing Coils

    NASA Technical Reports Server (NTRS)

    Hahn, Inseob; Penanen, Konstantin I.; Ho Eom, Byeong

    2008-01-01

    A heterogeneous material construction has been devised for sensing coils of superconducting quantum interference device (SQUID) magnetometers that are subject to a combination of requirements peculiar to some advanced applications, notably including low-field magnetic resonance imaging for medical diagnosis. The requirements in question are the following: The sensing coils must be large enough (in some cases having dimensions of as much as tens of centimeters) to afford adequate sensitivity; The sensing coils must be made electrically superconductive to eliminate Johnson noise (thermally induced noise proportional to electrical resistance); and Although the sensing coils must be cooled to below their superconducting- transition temperatures with sufficient cooling power to overcome moderate ambient radiative heat leakage, they must not be immersed in cryogenic liquid baths. For a given superconducting sensing coil, this combination of requirements can be satisfied by providing a sufficiently thermally conductive link between the coil and a cold source. However, the superconducting coil material is not suitable as such a link because electrically superconductive materials are typically poor thermal conductors. The heterogeneous material construction makes it possible to solve both the electrical- and thermal-conductivity problems. The basic idea is to construct the coil as a skeleton made of a highly thermally conductive material (typically, annealed copper), then coat the skeleton with an electrically superconductive alloy (typically, a lead-tin solder) [see figure]. In operation, the copper skeleton provides the required thermally conductive connection to the cold source, while the electrically superconductive coating material shields against Johnson noise that originates in the copper skeleton.

  10. Nanopottery: coiling of electrospun polymer nanofibers.

    PubMed

    Kim, Ho-Young; Lee, Minhee; Park, Kun Joong; Kim, Sungho; Mahadevan, L

    2010-06-01

    We show that a nanoscale polymer solution electrojet can coil to form free-standing hollow pottery as the jet is focused onto a sharp electrode tip. A scaling law is given based on the balance of the electrostatic compression force and the elastic resistance to predict the coil radius and frequency as the functions of relevant physical parameters. The structures formed by the nanofibers can be used in diverse fields of nanotechnology, for example, as nanomagnets, bioscaffolds, and nanochannels. PMID:20486713

  11. AC loss measurements in HTS coil assemblies with hybrid coil structures

    NASA Astrophysics Data System (ADS)

    Jiang, Zhenan; Long, Nicholas J.; Staines, Mike; Badcock, Rodney A.; Bumby, Chris W.; Buckley, Robert G.; Amemiya, Naoyuki

    2016-09-01

    Both AC loss and wire cost in coil windings are critical factors for high temperature superconductor (HTS) AC machinery applications. We present AC loss measurement results in three HTS coil assemblies at 77 K and 65 K which have a hybrid coil structure comprising one central winding (CW) and two end windings (EWs) wound with ReBCO and BSCCO wires with different self-field I c values at 77 K. All AC loss results in the coil assemblies are hysteretic and the normalized AC losses in the coil assemblies at different temperatures can be scaled with the I c value of the coil assemblies. The normalised results show that AC loss in a coil assembly with BSCCO CW can be reduced by using EWs wound with high I c ReBCO wires, whilst further AC loss reduction can be achieved by replacing the BSCCO CW with ReBCO CW. The results imply that a flexible hybrid coil structure is possible which considers both AC loss and wire cost in coil assemblies.

  12. pH sensitive coiled coils: a strategy for enhanced liposomal drug delivery

    NASA Astrophysics Data System (ADS)

    Reja, Rahi M.; Khan, Mohsina; Singh, Sumeet K.; Misra, Rajkumar; Shiras, Anjali; Gopi, Hosahudya N.

    2016-02-01

    Stimuli responsive controlled release from liposome based vesicles is a promising strategy for the site specific delivery of drugs. Herein, we report the design of pH sensitive coiled coils and their incorporation into the liposome as triggers for the controlled release of encapsulated drugs. The designed coiled coil peptides with the incorporation of environment sensitive fluorescent amino acids were found to be stable at physiological pH and unstructured while changing the pH of the environment to either acidic or basic. This pH dependent conformational switch of the coiled-coil polypeptides was exploited as triggers for the enhanced release of the encapsulated drug molecules from liposomes. The SEM, DLS and TEM analysis revealed the uniform morphology of the peptide liposome hybrid vesicles. Further, the drug encapsulated liposome internalization experiments with cancer cells revealed the enhanced release and accumulation of drugs in the acidic lysosomal compartments in comparison with liposomes without coiled coils.Stimuli responsive controlled release from liposome based vesicles is a promising strategy for the site specific delivery of drugs. Herein, we report the design of pH sensitive coiled coils and their incorporation into the liposome as triggers for the controlled release of encapsulated drugs. The designed coiled coil peptides with the incorporation of environment sensitive fluorescent amino acids were found to be stable at physiological pH and unstructured while changing the pH of the environment to either acidic or basic. This pH dependent conformational switch of the coiled-coil polypeptides was exploited as triggers for the enhanced release of the encapsulated drug molecules from liposomes. The SEM, DLS and TEM analysis revealed the uniform morphology of the peptide liposome hybrid vesicles. Further, the drug encapsulated liposome internalization experiments with cancer cells revealed the enhanced release and accumulation of drugs in the acidic

  13. Thermal Performance of the LDX Floating Coil

    NASA Astrophysics Data System (ADS)

    Zhukovsky, A.; Garnier, D. T.; Radovinsky, A. L.

    2006-04-01

    The Levitated Dipole Experiment (LDX) is an innovative facility to study plasma confinement in a dipole magnetic field, created by a superconducting solenoid (floating coil), which is magnetically levitated in the center of a 5 m diameter by 3 m tall vacuum chamber. The floating coil (F-coil) consists of a Nb3Sn magnet installed inside a strong vessel filled with high-pressure helium gas at room temperature. It is surrounded by a fiberglass-lead composite radiation shield and by a toroidal vacuum shell. The cryostat design provides the ability to operate the magnet for several hours of wanning while suspended in the middle of the vacuum chamber without electric and cryogenic connections to the coil. For this reason the magnet is charged/discharged inductively in a lower part of the vacuum chamber. The retractable cryogenic transfer lines serve to cool down the magnet to 4.5 K before it is lifted to the operating position. The F-coil can be re-cooled multiple times while maintaining its field and current. This paper describes the thermal performance of the F-coil.

  14. Image reconstructions with the rotating RF coil.

    PubMed

    Trakic, A; Wang, H; Weber, E; Li, B K; Poole, M; Liu, F; Crozier, S

    2009-12-01

    Recent studies have shown that rotating a single RF transceive coil (RRFC) provides a uniform coverage of the object and brings a number of hardware advantages (i.e. requires only one RF channel, averts coil-coil coupling interactions and facilitates large-scale multi-nuclear imaging). Motion of the RF coil sensitivity profile however violates the standard Fourier Transform definition of a time-invariant signal, and the images reconstructed in this conventional manner can be degraded by ghosting artifacts. To overcome this problem, this paper presents Time Division Multiplexed-Sensitivity Encoding (TDM-SENSE), as a new image reconstruction scheme that exploits the rotation of the RF coil sensitivity profile to facilitate ghost-free image reconstructions and reductions in image acquisition time. A transceive RRFC system for head imaging at 2 Tesla was constructed and applied in a number of in vivo experiments. In this initial study, alias-free head images were obtained in half the usual scan time. It is hoped that new sequences and methods will be developed by taking advantage of coil motion. PMID:19800824

  15. Image reconstructions with the rotating RF coil

    NASA Astrophysics Data System (ADS)

    Trakic, A.; Wang, H.; Weber, E.; Li, B. K.; Poole, M.; Liu, F.; Crozier, S.

    2009-12-01

    Recent studies have shown that rotating a single RF transceive coil (RRFC) provides a uniform coverage of the object and brings a number of hardware advantages (i.e. requires only one RF channel, averts coil-coil coupling interactions and facilitates large-scale multi-nuclear imaging). Motion of the RF coil sensitivity profile however violates the standard Fourier Transform definition of a time-invariant signal, and the images reconstructed in this conventional manner can be degraded by ghosting artifacts. To overcome this problem, this paper presents Time Division Multiplexed — Sensitivity Encoding (TDM-SENSE), as a new image reconstruction scheme that exploits the rotation of the RF coil sensitivity profile to facilitate ghost-free image reconstructions and reductions in image acquisition time. A transceive RRFC system for head imaging at 2 Tesla was constructed and applied in a number of in vivo experiments. In this initial study, alias-free head images were obtained in half the usual scan time. It is hoped that new sequences and methods will be developed by taking advantage of coil motion.

  16. Divertor Coil Design and Implementation on Pegasus

    NASA Astrophysics Data System (ADS)

    Shriwise, P. C.; Bongard, M. W.; Cole, J. A.; Fonck, R. J.; Kujak-Ford, B. A.; Lewicki, B. T.; Winz, G. R.

    2012-10-01

    An upgraded divertor coil system is being commissioned on the Pegasus Toroidal Experiment in conjunction with power system upgrades in order to achieve higher β plasmas, reduce impurities, and possibly achieve H-mode operation. Design points for the divertor coil locations and estimates of their necessary current ratings were found using predictive equilibrium modeling based upon a 300 kA target plasma. This modeling represented existing Pegasus coil locations and current drive limits. The resultant design calls for 125 kA-turns from the divertor system to support the creation of a double null magnetic topology in plasmas with Ip<=300 kA. Initial experiments using this system will employ 900 V IGBT power supply modules to provide IDIV<=4 kA. The resulting 20 kA-turn capability of the existing divertor coil will be augmented by a new coil providing additional A-turns in series. Induced vessel wall current modeling indicates the time response of a 28 turn augmentation coil remains fast compared to the poloidal field penetration rate through the vessel. First results operating the augmented system are shown.

  17. Auxiliary coil controls temperature of RF induction heater

    NASA Technical Reports Server (NTRS)

    1966-01-01

    Auxiliary coil controls the temperature of an RF induction furnace that is powered by a relatively unstable RF generator. Manual or servoed adjustments of the relative position of the auxiliary coil, which is placed in close proximity to the RF coil, changes the looseness of the RF coil and hence the corresponding heating effect of its RF field.

  18. Radiation-hard electrical coil and method for its fabrication

    DOEpatents

    Grieggs, R.J.; Blake, R.D.; Gac, F.D.

    1982-06-29

    A radiation-hard insulated electrical coil and method for making the same are disclosed. In accordance with the method, a conductor, preferably copper, is wrapped with an aluminum strip and then tightly wound into a coil. The aluminum-wrapped coil is then annealed to relax the conductor in the coiled configuration. The annealed coil is then immersed in an alkaline solution to dissolve the aluminum strip, leaving the bare conductor in a coiled configuration with all of the windings closely packed yet uniformly spaced from one another. The coil is then insulated with a refractory insulating material. In the preferred embodiment, the coil is insulated by coating it with a vitreous enamel and subsequently potting the enamelled coil in a castable ceramic concrete. The resulting coil is substantially insensitive to radiation and may be operated continuously in high radiation environments for long periods of time.

  19. Transient Heat Transfer in TCAP Coils

    SciTech Connect

    Steimke, J.L.

    1999-03-09

    The Thermal Cycling Absorption Process (TCAP) is used to separate isotopes of hydrogen. TCAP involves passing a stream of mixed hydrogen isotopes through palladium deposited on kieselguhr (Pd/k) while cycling the temperature of the Pd/k. Kieselguhr is a silica mineral also called diatomite. To aid in the design of a full scale facility, the Thermal Fluids Laboratory was used by the Chemical and Hydrogen Technology Section to compare the heat transfer properties of three different configurations of stainless steel coils containing kieselguhr and helium. Testing of coils containing Pd/k and hydrogen isotopes would have been more prototypical but would have been too expensive. Three stainless steel coils filled with kieselguhr were tested; one made from 2.0 inch diameter tubing, one made from 2.0 inch diameter tubing with foam copper embedded in the kieselguhr and one made from 1.25 inch diameter tubing. It was known prior to testing that increasing the tubing diameter from 1.25 inch to 2.0 inch would slow the rate of temperature change. The primary purpose of the testing was to measure to what extent the presence of copper foam in a 2.0" tubing coil would compensate for the effect of larger diameter. Each coil was connected to a pressure gage and the coil was evacuated and backfilled with helium gas. Helium was used instead of a mixture of hydrogen isotopes for reasons of safety. Each coil was quickly immersed in a stirred bath of ethylene glycol at a temperature of approximately 100 degrees Celsius. The coil pressure increased, reflecting the increase in average temperature of its contents. The pressure transient was recored as a function of time after immersion. Because of the actual process will use Pd/k instead of kieselguhr, additional tests were run to determine the differences in thermal properties between the two materials. The method was to position a thermocouple at the center of a hollow sphere and pack the sphere with Pd/k. The sphere was sealed, quickly

  20. Transient Heat Transfer in TCAP Coils

    SciTech Connect

    Steimke, J.L.

    1999-03-09

    The Thermal Cycling Absorption Process (TCAP) is used to separate isotopes of hydrogen. TCAP involves passing a stream of mixed hydrogen isotopes through palladium deposited on kieselguhr (Pd/k) while cycling the temperature of the Pd/k. Kieselguhr is a silica mineral also called diatomite. To aid in the design of a full scale facility, the Thermal Fluids Laboratory was used by the Chemical and Hydrogen Technology Section to compare the heat transfer properties of three different configurations of stainless steel coils containing kieselguhr and helium. Testing of coils containing Pd/k and hydrogen isotopes would have been more prototypical but would have been too expensive. Three stainless steel coils filled with kieselguhr were tested; one made from 2.0 inch diameter tubing, one made from 2.0 inch diameter tubing with foam copper embedded in the kieselguhr and one made from 1.25 inch diameter tubing. It was known prior to testing that increasing the tubing diameter from 1.25 inch to 2.0 inch would slow the rate of temperature change. The primary purpose of the testing was to measure to what extent the presence of copper foam in a 2.0 tubing coil would compensate for the effect of larger diameter. Each coil was connected to a pressure gage and the coil was evacuated and backfilled with helium gas. Helium was used instead of a mixture of hydrogen isotopes for reasons of safety. Each coil was quickly immersed in a stirred bath of ethylene glycol at a temperature of approximately 100 degrees Celsius. The coil pressure increased, reflecting the increase in average temperature of its contents. The pressure transient was recored as a function of time after immersion. Because of the actual process will use Pd/k instead of kieselguhr, additional tests were run to determine the differences in thermal properties between the two materials. The method was to position a thermocouple at the center of a hollow sphere and pack the sphere with Pd/k. The sphere was sealed, quickly

  1. Structural Correlation of the Neck Coil with the Coiled-coil (CC1)-Forkhead-associated (FHA) Tandem for Active Kinesin-3 KIF13A.

    PubMed

    Ren, Jinqi; Huo, Lin; Wang, Wenjuan; Zhang, Yong; Li, Wei; Lou, Jizhong; Xu, Tao; Feng, Wei

    2016-02-12

    Processive kinesin motors often contain a coiled-coil neck that controls the directionality and processivity. However, the neck coil (NC) of kinesin-3 is too short to form a stable coiled-coil dimer. Here, we found that the coiled-coil (CC1)-forkhead-associated (FHA) tandem (that is connected to NC by Pro-390) of kinesin-3 KIF13A assembles as an extended dimer. With the removal of Pro-390, the NC-CC1 tandem of KIF13A unexpectedly forms a continuous coiled-coil dimer that can be well aligned into the CC1-FHA dimer. The reverse introduction of Pro-390 breaks the NC-CC1 coiled-coil dimer but provides the intrinsic flexibility to couple NC with the CC1-FHA tandem. Mutations of either NC, CC1, or the FHA domain all significantly impaired the motor activity. Thus, the three elements within the NC-CC1-FHA tandem of KIF13A are structurally interrelated to form a stable dimer for activating the motor. This work also provides the first direct structural evidence to support the formation of a coiled-coil neck by the short characteristic neck domain of kinesin-3. PMID:26680000

  2. Gradient coil system for nuclear magnetic resonance apparatus

    SciTech Connect

    Frese, G.; Siebold, H.

    1984-08-28

    A gradient coil system for an image-generating, nuclear magnetic resonance tomographic apparatus, particularly a zeugmatographic apparatus. The gradient coil system is arranged on a support body of rotational symmetry, illustratively a hollow cylindrical support body, having an axis which extends along the z-direction of an x, y, z coordinate system which has an origin in the center of imaging region. The gradient coil system contains two pairs of toroidal individual coils which are arranged symmetrically with respect to an x-y plane which extends through the center of the imaging region and which are arranged perpendicular to the z-axis. The direction of current flow in the individual coils of a coil pair is opposite to the direction of flow in the individual coils of the other coil pair. Moreover, further sets of coils are provided for generating field gradient Gx in the x-direction, and Gy in the y-direction. The hollow cylindrical shape of the support body on which the individual coils are arranged permit an imaging region having a substantially spherical volume with a substantially constant field gradient Gz to be achieved. Each of the coils has a predetermined linkage factor which corresponds to the product of the current flowing through the number of coil turns of the coil. Those coils which are arranged further from the plane of symmetry have a substantially larger linkage factor than the coils which are nearer to the plane of symmetry.

  3. Self-Assembling Peptide-Polymer Hydrogels Designed From the Coiled Coil Region of Fibrin

    PubMed Central

    Jing, Peng; Rudra, Jai S.; Herr, Andrew B.; Collier, Joel H.

    2010-01-01

    Biomaterials constructed from self-assembling peptides, peptide derivatives, and peptide-polymer conjugates are receiving increasing attention as defined matrices for tissue engineering, controlled therapeutic release, and in vitro cell expansion, but many are constructed from peptide structures not typically found in the human extracellular matrix. Here we report a self-assembling biomaterial constructed from a designed peptide inspired by the coiled coil domain of human fibrin, the major protein constituent of blood clots and the provisional scaffold of wound healing. Targeted substitutions were made in the residues forming the interface between coiled coil strands for a 37-amino acid peptide from human fibrinogen to stabilize the coiled coil peptide bundle, while the solvent-exposed residues were left unchanged to provide a surface similar to that of the native protein. This peptide, which self-assembled into coiled coil dimers and tetramers, was then used to produce triblock peptide-PEG-peptide bioconjugates that self-assembled into viscoelastic hydrogel biomaterials. PMID:18712921

  4. Antiparallel Four-Stranded Coiled Coil Specified by a 3-3-1 Hyrdrophobic Heptad Repeat

    SciTech Connect

    Deng,Y.; Liu, J.; Zheng, Q.; Eliezer, D.; Kallenbach, N.; Lu, M.

    2006-01-01

    Coiled-coil sequences in proteins commonly share a seven-amino acid repeat with nonpolar side chains at the first (a) and fourth (d) positions. We investigate here the role of a 3-3-1 hydrophobic repeat containing nonpolar amino acids at the a, d, and g positions in determining the structures of coiled coils using mutants of the GCN4 leucine zipper dimerization domain. When three charged residues at the g positions in the parental sequence are replaced by nonpolar alanine or valine side chains, stable four-helix structures result. The X-ray crystal structures of the tetramers reveal antiparallel, four-stranded coiled coils in which the a, d, and g side chains interlock in a combination of knobs-into-knobs and knobs-into-holes packing. Interfacial interactions in a coiled coil can therefore be prescribed by hydrophobic-polar patterns beyond the canonical 3-4 heptad repeat. The results suggest that the conserved, charged residues at the g positions in the GCN4 leucine zipper can impart a negative design element to disfavor thermodynamically more stable, antiparallel tetramers.

  5. Self-sorting heterodimeric coiled coil peptides with defined and tuneable self-assembly properties

    PubMed Central

    Aronsson, Christopher; Dånmark, Staffan; Zhou, Feng; Öberg, Per; Enander, Karin; Su, Haibin; Aili, Daniel

    2015-01-01

    Coiled coils with defined assembly properties and dissociation constants are highly attractive components in synthetic biology and for fabrication of peptide-based hybrid nanomaterials and nanostructures. Complex assemblies based on multiple different peptides typically require orthogonal peptides obtained by negative design. Negative design does not necessarily exclude formation of undesired species and may eventually compromise the stability of the desired coiled coils. This work describe a set of four promiscuous 28-residue de novo designed peptides that heterodimerize and fold into parallel coiled coils. The peptides are non-orthogonal and can form four different heterodimers albeit with large differences in affinities. The peptides display dissociation constants for dimerization spanning from the micromolar to the picomolar range. The significant differences in affinities for dimerization make the peptides prone to thermodynamic social self-sorting as shown by thermal unfolding and fluorescence experiments, and confirmed by simulations. The peptides self-sort with high fidelity to form the two coiled coils with the highest and lowest affinities for heterodimerization. The possibility to exploit self-sorting of mutually complementary peptides could hence be a viable approach to guide the assembly of higher order architectures and a powerful strategy for fabrication of dynamic and tuneable nanostructured materials. PMID:26370878

  6. The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer

    PubMed Central

    Sanchez, Jacint G.; Okreglicka, Katarzyna; Chandrasekaran, Viswanathan; Welker, Jordan M.; Sundquist, Wesley I.; Pornillos, Owen

    2014-01-01

    Tripartite motif (TRIM) proteins make up a large family of coiled-coil-containing RING E3 ligases that function in many cellular processes, particularly innate antiviral response pathways. Both dimerization and higher-order assembly are important elements of TRIM protein function, but the atomic details of TRIM tertiary and quaternary structure have not been fully understood. Here, we present crystallographic and biochemical analyses of the TRIM coiled-coil and show that TRIM proteins dimerize by forming interdigitating antiparallel helical hairpins that position the N-terminal catalytic RING domains at opposite ends of the dimer and the C-terminal substrate-binding domains at the center. The dimer core comprises an antiparallel coiled-coil with a distinctive, symmetric pattern of flanking heptad and central hendecad repeats that appear to be conserved across the entire TRIM family. Our studies reveal how the coiled-coil organizes TRIM25 to polyubiquitylate the RIG-I/viral RNA recognition complex and how dimers of the TRIM5α protein are arranged within hexagonal arrays that recognize the HIV-1 capsid lattice and restrict retroviral replication. PMID:24550273

  7. A coiled-coil domain acts as a molecular ruler in LPS chain length regulation

    PubMed Central

    Tuukkanen, Anne; Danciu, Iulia; Svergun, Dmitri I.; Hussain, Rohanah; Liu, Huanting; Whitfield, Chris; Naismith, James H.

    2014-01-01

    Long-chain bacterial polysaccharides play important roles in pathogenicity. In Escherichia coli O9a, a model for ABC transporter dependent polysaccharide assembly, a large extracellular carbohydrate with a narrow distribution of size is polymerized from monosaccharides by a complex of two proteins, WbdA (polymerase) and WbdD (terminating protein). Such careful control of polymerization is recurring theme in biology. Combining crystallography and small angle X-ray scattering, we show that the C-terminal domain of WbdD contains an extended coiled-coil that physically separates WbdA from the catalytic domain of WbdD. The effects of insertions and deletions within the coiled-coil region were analyzed in vivo, revealing that polymer size is controlled by varying the length of the coiled-coil domain. Thus, the coiled-coil domain of WbdD functions as a molecular ruler that, along with WbdA:WbdD stoichiometry, controls the chain length of a model bacterial polysaccharide. PMID:25504321

  8. Forced Unfolding of the Coiled-Coils of Fibrinogen by Single-Molecule AFM

    NASA Astrophysics Data System (ADS)

    Brown, Andre; Litvinov, Rustem; Discher, Dennis; Weisel, John

    2007-03-01

    A blood clot needs to have the right degree of stiffness and plasticity for hemostasis, but the origin of these mechanical properties is unknown. Here we report the first measurements using single molecule atomic force microscopy (AFM) to study the forced unfolding of fibrinogen to begin addressing this problem. To generate longer reproducible curves than are possible using monomer, factor XIIIa cross-linked, single chain fibrinogen oligomers were used. When extended under force, these oligomers showed sawtooth shaped force-extension patterns characteristic of unfolding proteins with a peak-to-peak separation of approximately 26 nm, consistent with the independent unfolding of the coiled-coils. These results were then reproduced using a Monte Carlo simulation with parameters in the same range as those previously used for unfolding globular domains. In particular, we found that the refolding time was negligible on experimental time and force scales in contrast to previous work on simpler coiled-coils. We suggest that this difference may be due to fibrinogen's structurally and topologically more complex coiled-coils and that an interaction between the alpha C and central domains may be involved. These results suggest a new functional property of fibrinogen and that the coiled-coil is more than a passive structural element of this molecule.

  9. Coil in coil - components for the high voltage superconducting resistive current limiter CULT 110

    NASA Astrophysics Data System (ADS)

    Elschner, S.; Stemmle, M.; Breuer, F.; Walter, H.; Frohne, C.; Noe, M.; Bock, J.

    2008-02-01

    The German government (BMBF/VDI) funded project CULT 110 is presently the largest European current limiter project and aims at the development of a one-phase resistive limiter for the voltage level of 110 kV. The contribution presents the actual state of development of the superconducting components. As in the successful predecessor project CURL 10 these are made of melt cast processed BSCCO 2212 bulk material, however monofilar instead of bifilar coils are used. The electrical protection concept is based on a normal conducting coil arranged around a superconducting coil and connected in parallel. Simultaneously this coil serves as an electrical bypass and, under fault conditions, generates a magnetic field for quench homogenisation. Since no continuously connected shunt is needed, a much higher voltage during faults can be applied. The rules for an optimum superconductor and coil design are given and the viability of the whole concept is demonstrated by both, experiment and numerical simulation.

  10. Residual Stress Measurement Using Rectangular Spiral Coils

    NASA Astrophysics Data System (ADS)

    Sun, Haiyan; Plotnikov, Yuri

    2008-02-01

    Shot peening process provides compressive residual stress within a depth of about 150˜200 um from the surface. It has been demonstrated that multi-frequency eddy current measurement can be effectively used for the residual stress estimation on Ni-based superalloys. In order to measure the stress profile over the entire compressive zone, the probe needs to work in a wide frequency range from 0.1 MHz to above 50 MHz. Due to its wide bandwidth and high precision fabrication process, spiral coils fabricated on flexible substrate using photolithographic technology are good candidate for this task It is useful to develop a coil model in order to optimize coil design, minimize liftoff effect and maximize coil gauge factor. In this work, a 3D analytical model was used to simulate rectangular spiral coil response on a half-space conductor. The results were compared with commercial available 3D finite element software and experimental results. The analytical model was also used to simulate 4-point calibration process that was used to calculate apparent eddy current conductivity (AECC). The experimental setup was described and AECC profile was obtained for shot-peening samples with different peening intensity and different heat treatment.

  11. Acoustic rainbow trapping by coiling up space.

    PubMed

    Ni, Xu; Wu, Ying; Chen, Ze-Guo; Zheng, Li-Yang; Xu, Ye-Long; Nayar, Priyanka; Liu, Xiao-Ping; Lu, Ming-Hui; Chen, Yan-Feng

    2014-01-01

    We numerically realize the acoustic rainbow trapping effect by tapping an air waveguide with space-coiling metamaterials. Due to the high refractive-index of the space-coiling metamaterials, our device is more compact compared to the reported trapped-rainbow devices. A numerical model utilizing effective parameters is also calculated, whose results are consistent well with the direct numerical simulation of space-coiling structure. Moreover, such device with the capability of dropping different frequency components of a broadband incident temporal acoustic signal into different channels can function as an acoustic wavelength division de-multiplexer. These results may have potential applications in acoustic device design such as an acoustic filter and an artificial cochlea. PMID:25392033

  12. Output beam analysis of high power COIL

    NASA Astrophysics Data System (ADS)

    Yu, Deli; Sang, Fengting; Jin, Yuqi; Sun, Yizhu

    2003-03-01

    As the output power of a chemical oxygen iodine laser (COIL) increases, the output laser beam instability appears as the far-field beam spot drift and deformation for the large Fresnel number unstable resonator. In order to interpret this phenomenon, an output beam mode simulation code was developed with the fast Fourier transform method. The calculation results show that the presence of the nonuniform gain in COIL produces a skewed output intensity distribution, which causes the mirror tilt and bulge due to the thermal expansion. With the output power of COIL increases, the mirror surfaces, especially the back surface of the scraper mirror, absorb more and more heat, which causes the drift and deformation of far field beam spot seriously. The initial misalignment direction is an important factor for the far field beam spot drifting and deformation.

  13. Gas Filled Coaxial Accelerator with Compression Coil

    NASA Technical Reports Server (NTRS)

    Espy, Patrick N. (Inventor)

    1976-01-01

    A self-energized plasma compressor which compresses plasma discharged from a coaxial plasma generator. The device includes a helical shaped coil which is coaxially aligned with the center axis of the coaxial plasma generator. The plasma generator creates a current through the helical coil which, in turn, generates a time varying magnetic field that generates a force which acts radially upon the plasma. A seal is carried on the end of the coaxial plasma generator for containing gas therein. As the plasma is accelerated out the outer end of the generator, it forces the gas outwardly also compressing such. Beads are carried adjacent the small end of the helical shaped coil for being accelerated to hypervelocities by the plasma and gas. As a result of utilizing gas in the coaxial plasma generator, such minimizes ablation of the beads as well as accelerates such to higher velocities.

  14. Coupled wave model for large magnet coils

    NASA Technical Reports Server (NTRS)

    Gabriel, G. J.

    1980-01-01

    A wave coupled model based on field theory is evolved for analysis of fast electromagnetic transients on superconducting coils. It is expected to play a useful role in the design of protection methods against damage due to high voltages or any adverse effects that might arise from unintentional transients. The significant parameters of the coil are identified to be the turn to turn wave coupling coefficients and the travel time of an electromagnetic disturbance around a single turn. Unlike circuit theoretic inductor, the coil response evolves in discrete steps having durations equal to this travel time. It is during such intervals that high voltages are likely to occur. The model also bridges the gap between the low and high ends of the frequency spectrum.

  15. Measuring the orthogonality error of coil systems

    USGS Publications Warehouse

    Heilig, B.; Csontos, A.; Pajunpää, K.; White, Tim; St. Louis, B.; Calp, D.

    2012-01-01

    Recently, a simple method was proposed for the determination of pitch angle between two coil axes by means of a total field magnetometer. The method is applicable when the homogeneous volume in the centre of the coil system is large enough to accommodate the total field sensor. Orthogonality of calibration coil systems used for calibrating vector magnetometers can be attained by this procedure. In addition, the method can be easily automated and applied to the calibration of delta inclination–delta declination (dIdD) magnetometers. The method was tested by several independent research groups, having a variety of test equipment, and located at differing geomagnetic observatories, including: Nurmijärvi, Finland; Hermanus, South Africa; Ottawa, Canada; Tihany, Hungary. This paper summarizes the test results, and discusses the advantages and limitations of the method.

  16. Acoustic rainbow trapping by coiling up space

    PubMed Central

    Ni, Xu; Wu, Ying; Chen, Ze-Guo; Zheng, Li-Yang; Xu, Ye-Long; Nayar, Priyanka; Liu, Xiao-Ping; Lu, Ming-Hui; Chen, Yan-Feng

    2014-01-01

    We numerically realize the acoustic rainbow trapping effect by tapping an air waveguide with space-coiling metamaterials. Due to the high refractive-index of the space-coiling metamaterials, our device is more compact compared to the reported trapped-rainbow devices. A numerical model utilizing effective parameters is also calculated, whose results are consistent well with the direct numerical simulation of space-coiling structure. Moreover, such device with the capability of dropping different frequency components of a broadband incident temporal acoustic signal into different channels can function as an acoustic wavelength division de-multiplexer. These results may have potential applications in acoustic device design such as an acoustic filter and an artificial cochlea. PMID:25392033

  17. Dual Frequency Coil Array for MR Imaging

    NASA Astrophysics Data System (ADS)

    Amador-Baheza, R.; Sacristan-Rock, E.; Rodríguez, A. O.

    2002-08-01

    An array coil to perform in vivo Magnetic Resonance Imaging and Spectroscopy was developed to study the intestinal wall. It consisted of two surface rectangular-shaped coils mounted on cylindrical structure forming an orthogonal assembly. Since this design is intended to generate images and spectra, each element was tuned to a different resonant frequency: a) imaging: 200 MHz (1H) and b) spectroscopy: 81 MHz (31P). However, at this stage of the research, imaging experiments were only conducted on a Bruker 4.7 Tesla animal system. High-resolution images were obtained from a saline filled phantom and from the intestinal wall of a fully anaesthetised rabbit. The dual frequency coil array can be used to study the pathophysiology of intestinal ischemia.

  18. Superconductor coil geometry and ac losses

    NASA Technical Reports Server (NTRS)

    Pierce, T. V., Jr.; Zapata, R. N.

    1976-01-01

    An empirical relation is presented which allows simple computation of volume-averaged winding fields from central fields for coils of small rectangular cross sections. This relation suggests that, in certain applications, ac-loss minimization can be accomplished by use of low winding densities, provided that hysteresis losses are independent of winding density. The ac-loss measurements on coils wound of twisted multifilamentary composite superconductors show no significant dependence on ac losses on winding density, thus permitting the use of winding density as an independent design parameter in loss minimization.

  19. Coiled coil interactions for the targeting of liposomes for nucleic acid delivery

    NASA Astrophysics Data System (ADS)

    Oude Blenke, Erik E.; van den Dikkenberg, Joep; van Kolck, Bartjan; Kros, Alexander; Mastrobattista, Enrico

    2016-04-01

    Coiled coil interactions are strong protein-protein interactions that are involved in many biological processes, including intracellular trafficking and membrane fusion. A synthetic heterodimeric coiled-coil forming peptide pair, known as E3 (EIAALEK)3 and K3 (KIAALKE)3 was used to functionalize liposomes encapsulating a splice correcting oligonucleotide or siRNA. These peptide-functionalized vesicles are highly stable in solution but start to cluster when vesicles modified with complementary peptides are mixed together, demonstrating that the peptides quickly coil and crosslink the vesicles. When one of the peptides was anchored to the cell membrane using a hydrophobic cholesterol anchor, vesicles functionalized with the complementary peptide could be docked to these cells, whereas non-functionalized cells did not show any vesicle tethering. Although the anchored peptides do not have a downstream signaling pathway, microscopy pictures revealed that after four hours, the majority of the docked vesicles were internalized by endocytosis. Finally, for the first time, it was shown that the coiled coil assembly at the interface between the vesicles and the cell membrane induces active uptake and leads to cytosolic delivery of the nucleic acid cargo. Both the siRNA and the splice correcting oligonucleotide were functionally delivered, resulting respectively in the silencing or recovery of luciferase expression in the appropriate cell lines. These results demonstrate that the docking to the cell by coiled coil interaction can induce active uptake and achieve the successful intracellular delivery of otherwise membrane impermeable nucleic acids in a highly specific manner.Coiled coil interactions are strong protein-protein interactions that are involved in many biological processes, including intracellular trafficking and membrane fusion. A synthetic heterodimeric coiled-coil forming peptide pair, known as E3 (EIAALEK)3 and K3 (KIAALKE)3 was used to functionalize liposomes

  20. Coiled coil interactions for the targeting of liposomes for nucleic acid delivery

    NASA Astrophysics Data System (ADS)

    Oude Blenke, Erik E.; van den Dikkenberg, Joep; van Kolck, Bartjan; Kros, Alexander; Mastrobattista, Enrico

    2016-04-01

    Coiled coil interactions are strong protein-protein interactions that are involved in many biological processes, including intracellular trafficking and membrane fusion. A synthetic heterodimeric coiled-coil forming peptide pair, known as E3 (EIAALEK)3 and K3 (KIAALKE)3 was used to functionalize liposomes encapsulating a splice correcting oligonucleotide or siRNA. These peptide-functionalized vesicles are highly stable in solution but start to cluster when vesicles modified with complementary peptides are mixed together, demonstrating that the peptides quickly coil and crosslink the vesicles. When one of the peptides was anchored to the cell membrane using a hydrophobic cholesterol anchor, vesicles functionalized with the complementary peptide could be docked to these cells, whereas non-functionalized cells did not show any vesicle tethering. Although the anchored peptides do not have a downstream signaling pathway, microscopy pictures revealed that after four hours, the majority of the docked vesicles were internalized by endocytosis. Finally, for the first time, it was shown that the coiled coil assembly at the interface between the vesicles and the cell membrane induces active uptake and leads to cytosolic delivery of the nucleic acid cargo. Both the siRNA and the splice correcting oligonucleotide were functionally delivered, resulting respectively in the silencing or recovery of luciferase expression in the appropriate cell lines. These results demonstrate that the docking to the cell by coiled coil interaction can induce active uptake and achieve the successful intracellular delivery of otherwise membrane impermeable nucleic acids in a highly specific manner.Coiled coil interactions are strong protein-protein interactions that are involved in many biological processes, including intracellular trafficking and membrane fusion. A synthetic heterodimeric coiled-coil forming peptide pair, known as E3 (EIAALEK)3 and K3 (KIAALKE)3 was used to functionalize liposomes

  1. Dynamics of the coiled-coil unfolding transition of myosin rod probed by dissipation force spectrum.

    PubMed

    Taniguchi, Yukinori; Khatri, Bhavin S; Brockwell, David J; Paci, Emanuele; Kawakami, Masaru

    2010-07-01

    The motor protein myosin II plays a crucial role in muscle contraction. The mechanical properties of its coiled-coil region, the myosin rod, are important for effective force transduction during muscle function. Previous studies have investigated the static elastic response of the myosin rod. However, analogous to the study of macroscopic complex fluids, how myosin will respond to physiological time-dependent loads can only be understood from its viscoelastic response. Here, we apply atomic force microscopy using a magnetically driven oscillating cantilever to measure the dissipative properties of single myosin rods that provide unique dynamical information about the coiled-coil structure as a function of force. We find that the friction constant of the single myosin rod has a highly nontrivial variation with force; in particular, the single-molecule friction constant is reduced dramatically and increases again as it passes through the coiled-uncoiled transition. This is a direct indication of a large free-energy barrier to uncoiling, which may be related to a fine-tuned dynamic mechanosignaling response to large and unexpected physiological loads. Further, from the critical force at which the minimum in friction occurs we determine the asymmetry of the bistable landscape that controls uncoiling of the coiled coil. This work highlights the sensitivity of the dissipative signal in force unfolding to dynamic molecular structure that is hidden to the elastic signal. PMID:20655854

  2. Resistive demountable toroidal-field coils for tokamak reactors

    SciTech Connect

    Jassby, D.L.; Jacobsen, R.A.; Kalnavarns, J.; Masson, L.S.; Sekot, J.P.

    1981-07-01

    Readily demountable TF (toroidal-field) coils allow complete access to the internal components of a tokamak reactor for maintenance of replacement. The requirement of readily demountable joints dictates the use of water-cooled resistive coils, which have a host of decisive advantages over superconducting coils. Previous papers have shown that resistive TF coils for tokamak reactors can operate in the steady state with acceptable power dissipation (typically, 175 to 300 MW). This paper summarizes results of parametric studies of size optimization of rectangular TF coils and of a finite-element stress analysis, and examines several candidate methods of implementing demountable joints for rectangular coils constructed of plate segments.

  3. Characteristics of bowl-shaped coils for transcranial magnetic stimulation

    NASA Astrophysics Data System (ADS)

    Yamamoto, Keita; Suyama, Momoko; Takiyama, Yoshihiro; Kim, Dongmin; Saitoh, Youichi; Sekino, Masaki

    2015-05-01

    Transcranial magnetic stimulation (TMS) has recently been used as a method for the treatment of neurological and psychiatric diseases. Daily TMS sessions can provide continuous therapeutic effectiveness, and the installation of TMS systems at patients' homes has been proposed. A figure-eight coil, which is normally used for TMS therapy, induces a highly localized electric field; however, it is challenging to achieve accurate coil positioning above the targeted brain area using this coil. In this paper, a bowl-shaped coil for stimulating a localized but wider area of the brain is proposed. The coil's electromagnetic characteristics were analyzed using finite element methods, and the analysis showed that the bowl-shaped coil induced electric fields in a wider area of the brain model than a figure-eight coil. The expanded distribution of the electric field led to greater robustness of the coil to the coil-positioning error. To improve the efficiency of the coil, the relationship between individual coil design parameters and the resulting coil characteristics was numerically analyzed. It was concluded that lengthening the outer spherical radius and narrowing the width of the coil were effective methods for obtaining a more effective and more uniform distribution of the electric field.

  4. Tool Removes Coil-Spring Thread Inserts

    NASA Technical Reports Server (NTRS)

    Collins, Gerald J., Jr.; Swenson, Gary J.; Mcclellan, J. Scott

    1991-01-01

    Tool removes coil-spring thread inserts from threaded holes. Threads into hole, pries insert loose, grips insert, then pulls insert to thread it out of hole. Effects essentially reverse of insertion process to ease removal and avoid further damage to threaded inner surface of hole.

  5. Simplified deflection-coil linearity testing

    NASA Technical Reports Server (NTRS)

    Kramer, G. P.

    1976-01-01

    Mask placed over face of image-dissecting photomultiplier tube has precision array of pinholes that permit light to impinge on tube at known points. Signals are fed to deflection coil which sweeps beam across each point without complex operator procedures.

  6. High-field superconducting nested coil magnet

    NASA Technical Reports Server (NTRS)

    Laverick, C.; Lobell, G. M.

    1970-01-01

    Superconducting magnet, employed in conjunction with five types of superconducting cables in a nested solenoid configuration, produces total, central magnetic field strengths approaching 70 kG. The multiple coils permit maximum information on cable characteristics to be gathered from one test.

  7. Transcatheter Coil Embolization of Splenic Artery Aneurysm

    SciTech Connect

    Yamamoto, Satoshi Hirota, Shozo; Maeda, Hiroaki; Achiwa, Sachiko Arai, Keisuke; Kobayashi, Kaoru; Nakao, Norio

    2008-05-15

    The purpose of this study was to evaluate clinical results and technical problems of transcatheter coil embolization for splenic artery aneurysm. Subjects were 16 patients (8 men, 8 women; age range, 40-80 years) who underwent transcatheter embolization for splenic artery aneurysm (14 true aneurysms, 2 false aneurysms) at one of our hospitals during the period January 1997 through July 2005. Two aneurysms (12.5%) were diagnosed at the time of rupture. Multiple splenic aneurysms were found in seven patients. Aneurysms were classified by site as proximal (or strictly ostial) (n = 3), middle (n = 3), or hilar (n = 10). The indication for transcatheter arterial embolization was a false or true aneurysm 20 mm in diameter. Embolic materials were fibered coils and interlocking detachable coils. Embolization was performed by the isolation technique, the packing technique, or both. Technically, all aneurysms were devascularized without severe complications. Embolized aneurysms were 6-40 mm in diameter (mean, 25 mm). Overall, the primary technical success rate was 88% (14 of 16 patients). In the remaining 2 patients (12.5%), partial recanalization occurred, and re-embolization was performed. The secondary technical success rate was 100%. Seven (44%) of the 16 study patients suffered partial splenic infarction. Intrasplenic branching originating from the aneurysm was observed in five patients. We conclude that transcatheter coil embolization should be the initial treatment of choice for splenic artery aneurysm.

  8. COMPACT COILED DENUDER FOR ATMOSPHERIC SAMPLING

    EPA Science Inventory

    A compact coiled denuder has been designed and its performance evaluated both theoretically and experimentally. he design is based on special features of laminar flow in a curved tube, which significantly enhance the mass transfer Sherwood number governing gas collection at the w...

  9. 49 CFR 236.730 - Coil, receiver.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., MAINTENANCE, AND REPAIR OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Definitions § 236.730 Coil, receiver. Concentric layers of insulated wire wound around the core of a receiver of an automatic train stop, train control or cab signal device on a locomotive....

  10. Stellarator Coil Design and Plasma Sensitivity

    SciTech Connect

    Long-Poe Ku and Allen H. Boozer

    2010-11-03

    The rich information contained in the plasma response to external magnetic perturbations can be used to help design stellarator coils more effectively. We demonstrate the feasibility by first devel- oping a simple, direct method to study perturbations in stellarators that do not break stellarator symmetry and periodicity. The method applies a small perturbation to the plasma boundary and evaluates the resulting perturbed free-boundary equilibrium to build up a sensitivity matrix for the important physics attributes of the underlying configuration. Using this sensitivity information, design methods for better stellarator coils are then developed. The procedure and a proof-of-principle application are given that (1) determine the spatial distributions of external normal magnetic field at the location of the unperturbed plasma boundary to which the plasma properties are most sen- sitive, (2) determine the distributions of external normal magnetic field that can be produced most efficiently by distant coils, (3) choose the ratios of the magnitudes of the the efficiently produced magnetic distributions so the sensitive plasma properties can be controlled. Using these methods, sets of modular coils are found for the National Compact Stellarator Experiment (NCSX) that are either smoother or can be located much farther from the plasma boundary than those of the present design.

  11. Modular Coils and Plasma Configurations for Quasi-axisymmetric Stellarators

    SciTech Connect

    L.P. Ku and A.H. Boozer

    2010-09-10

    Characteristics of modular coils for quasi-axisymmetric stellarators that are related to the plasma aspect ratio, number of field periods and rotational transform have been examined systematically. It is observed that, for a given plasma aspect ratio, the coil complexity tends to increase with the increased number of field periods. For a given number of field periods, the toroidal excursion of coil winding is reduced as the plasma aspect ratio is increased. It is also clear that the larger the coil-plasma separation is, the more complex the coils become. It is further demonstrated that it is possible to use other types of coils to complement modular coils to improve both the physics and the modular coil characteristics.

  12. Mechanical design of a high field common coil magnet

    SciTech Connect

    Caspi, S.; Chow, K.; Dietderich, D.; Gourlay, S.; Gupta, R.; McInturff, A.; Millos, G.; Scanlan, R.

    1999-03-18

    A common coil design for high field 2-in-1 accelerator magnets has been previously presented as a 'conductor-friendly' option for high field magnets applicable for a Very Large Hadron Collider. This paper presents the mechanical design for a 14 tesla 2-in-1 dipole based on the common coil design approach. The magnet will use a high current density Nb{sub 3}Sn conductor. The design addresses mechanical issues particular to the common coil geometry: horizontal support against coil edges, vertical preload on coil faces, end loading and support, and coil stresses and strains. The magnet is the second in a series of racetrack coil magnets that will provide experimental verification of the common coil design approach.

  13. Noise properties of a NMR transceiver coil array.

    PubMed

    Pinkerton, Robert G; Barberi, Enzo A; Menon, Ravi S

    2004-11-01

    The use of multiple radiofrequency (RF) surface coil elements has applications in both fast parallel imaging and conventional imaging techniques. Through implementation of a simple magnetic decoupling network, 50 Omega matching can be achieved in both the transmitter and receiver chains, enabling the use of conventional RF power amplifiers and preamplifiers for transceive applications. Unlike phased array coil arrangements using low impedance preamplifiers for decoupling, the noise correlation between 50 Omega coils decoupled with discrete components has not been characterized. We have measured the dependence of coil quality factor (Q-factor) and noise correlation on coil separation and shown these quantities to be consistent with theoretical arguments, at least at 4 T (170 MHz). Our results suggest that a coil system for transmission and reception of NMR signals with 50 Omega coils can be built to take advantage of all the benefits of conventional array coils and with the added advantages of using conventional amplifiers. PMID:15504694

  14. Particle confinement in EBT reactors with noncircular mirror coils

    SciTech Connect

    Owen, L.W.; Uckan, N.A.

    1983-01-01

    Methods of improving single particle confinement in the vacuum magnetic field of an ELMO Bumpy Torus (EBT) reactor have heretofore focused on enhancement of the effective magnetic aspect ratio through the addition of relatively low current supplementary coils to the basic EBT configuration of toroidally linked circular mirror coils. This method of aspect ratio enhancement is reviewed and compared to the use of noncircular, D-shaped mirror coils. A critical parameter in this evaluation is the required radial thickness delta of the blanket-shield assembly in the coil throat. Results indicate that D-coils represent an attractive alternative to the supplementary coil configurations if future neutronics calculations show that delta coils. D-coils are shown to be extremely effective in symmetrizing mod-B in the midplane, thereby giving good trapped particle confinement, hot electron ring centering, and reactor volume utilization.

  15. Coiled-Coil Irregularities and Instabilities in Group A Streptococcus M1 Are Required for Virulence

    SciTech Connect

    McNamara, Case; Zinkernagel, Annelies S.; Macheboeuf, Pauline; Cunningham, Madeleine W.; Nizet, Victor; Ghosh, Partho

    2008-07-21

    Antigenically variable M proteins are major virulence factors and immunogens of the human pathogen group A Streptococcus (GAS). Here, we report the -3 angstrom resolution structure of a GAS M1 fragment containing the regions responsible for eliciting type-specific, protective immunity and for binding fibrinogen, which promotes M1 proinflammatory and antiphagocytic functions. The structure revealed substantial irregularities and instabilities throughout the coiled coil of the M1 fragment. Similar structural irregularities occur in myosin and tropomyosin, explaining the patterns of cross-reactivity seen in autoimmune sequelae of GAS infection. Sequence idealization of a large segment of the M1 coiled coil enhanced stability but diminished fibrinogen binding, proinflammatory effects, and antibody cross-reactivity, whereas it left protective immunogenicity undiminished. Idealized M proteins appear to have promise as vaccine immunogens.

  16. Comparison of an Electromagnetic Energy Harvester Performance using Wound Coil Wire and PCB Coil

    NASA Astrophysics Data System (ADS)

    Resali, MSM; Salleh, H.

    2016-03-01

    This paper presents the performance of two types of electromagnetic energy harvester, one using manually wound coil wire (EH-EC) and the other one using printed circuit board (PCB) coil (EH-EP). The objective of the study is to measure the corresponding output voltage and power by varying the number of coils and the position of the magnet. The experiment was conducted at a fix 50 Hz of frequency and at 0.25g of acceleration. The EH-EP was found to be more effective than the 350 turns of the wound coil wire, with maximum power of 26 μW. Overall, the performance of the EH-EC showed better result with maximum power of 125 μW for 1050 turns when compared to the EH-EP.

  17. Short peptide tag for covalent protein labeling based on coiled coils.

    PubMed

    Wang, Jianpeng; Yu, Yongsheng; Xia, Jiang

    2014-01-15

    To label proteins covalently, one faces a trade-off between labeling a protein specifically and using a small tag. Often one must compromise one parameter for the other or use additional components, such as an enzyme, to satisfy both requirements. Here, we report a new reaction that covalently labels proteins by using engineered coiled-coil peptides. Harnessing the concept of "proximity-induced reactivity", the 21-amino-acid three-heptad peptides CCE/CCK were modified with a nucleophilic cysteine and an α-chloroacetyl group at selected positions. When pairs of coiled coils associated, an irreversible covalent bond spontaneously formed between the peptides. The specificity of the cross-linking reaction was characterized, the probes were improved by making them bivalent, and the system was used to label a protein in vitro and receptors on the surface of mammalian cells. PMID:24341800

  18. GBNV encoded movement protein (NSm) remodels ER network via C-terminal coiled coil domain

    SciTech Connect

    Singh, Pratibha; Savithri, H.S.

    2015-08-15

    Plant viruses exploit the host machinery for targeting the viral genome–movement protein complex to plasmodesmata (PD). The mechanism by which the non-structural protein m (NSm) of Groundnut bud necrosis virus (GBNV) is targeted to PD was investigated using Agrobacterium mediated transient expression of NSm and its fusion proteins in Nicotiana benthamiana. GFP:NSm formed punctuate structures that colocalized with mCherry:plasmodesmata localized protein 1a (PDLP 1a) confirming that GBNV NSm localizes to PD. Unlike in other movement proteins, the C-terminal coiled coil domain of GBNV NSm was shown to be involved in the localization of NSm to PD, as deletion of this domain resulted in the cytoplasmic localization of NSm. Treatment with Brefeldin A demonstrated the role of ER in targeting GFP NSm to PD. Furthermore, mCherry:NSm co-localized with ER–GFP (endoplasmic reticulum targeting peptide (HDEL peptide fused with GFP). Co-expression of NSm with ER–GFP showed that the ER-network was transformed into vesicles indicating that NSm interacts with ER and remodels it. Mutations in the conserved hydrophobic region of NSm (residues 130–138) did not abolish the formation of vesicles. Additionally, the conserved prolines at positions 140 and 142 were found to be essential for targeting the vesicles to the cell membrane. Further, systematic deletion of amino acid residues from N- and C-terminus demonstrated that N-terminal 203 amino acids are dispensable for the vesicle formation. On the other hand, the C-terminal coiled coil domain when expressed alone could also form vesicles. These results suggest that GBNV NSm remodels the ER network by forming vesicles via its interaction through the C-terminal coiled coil domain. Interestingly, NSm interacts with NP in vitro and coexpression of these two proteins in planta resulted in the relocalization of NP to PD and this relocalization was abolished when the N-terminal unfolded region of NSm was deleted. Thus, the NSm

  19. IMPROVED COILED-COIL DESIGN ENHANCES INTERACTION WITH BCR-ABL AND INDUCES APOPTOSIS

    PubMed Central

    Dixon, Andrew S.; Miller, Geoffrey D.; Bruno, Benjamin J.; Constance, Jonathan E.; Woessner, David W.; Fidler, Trevor P.; Robertson, James C.; Cheatham, Thomas E.; Lim, Carol S.

    2012-01-01

    The oncoprotein Bcr-Abl drives aberrant downstream activity through trans-autophosphorylation of homo-oligomers in chronic myelogenous leukemia (CML).1,2 The formation of Bcr-Abl oligomers is achieved through the coiled-coil domain at the N-terminus of Bcr.3, 4 We have previously reported a modified version of this coiled-coil domain, CCmut2, which exhibits disruption of Bcr-Abl oligomeric complexes and results in decreased proliferation of CML cells and induction of apoptosis.5 A major contributing factor to these enhanced capabilities is the destabilization of the CCmut2 homo-dimers, increasing the availability to interact with and inhibit Bcr-Abl. Here, we included an additional mutation (K39E) that could in turn further destabilize the mutant homo-dimer. Incorporation of this modification into CCmut2 (C38A, S41R, L45D, E48R, Q60E) generated what we termed CCmut3, and resulted in further improvements in the binding properties with the wild-type coiled-coil domain representative of Bcr-Abl. A separate construct containing one revert mutation, CCmut4, did not demonstrate improved oligomeric properties and indicated the importance of the L45D mutation. CCmut3 demonstrated improved oligomerization via a two-hybrid assay as well as through colocalization studies, in addition to showing similar biologic activity as CCmut2. The improved binding between CCmut3 and the Bcr-Abl coiled-coil may be used to redirect Bcr-Abl to alternative subcellular locations with interesting therapeutic implications. PMID:22136227

  20. Data-Driven Prediction and Design of bZIP Coiled-Coil Interactions

    PubMed Central

    Potapov, Vladimir; Kaplan, Jenifer B.; Keating, Amy E.

    2015-01-01

    Selective dimerization of the basic-region leucine-zipper (bZIP) transcription factors presents a vivid example of how a high degree of interaction specificity can be achieved within a family of structurally similar proteins. The coiled-coil motif that mediates homo- or hetero-dimerization of the bZIP proteins has been intensively studied, and a variety of methods have been proposed to predict these interactions from sequence data. In this work, we used a large quantitative set of 4,549 bZIP coiled-coil interactions to develop a predictive model that exploits knowledge of structurally conserved residue-residue interactions in the coiled-coil motif. Our model, which expresses interaction energies as a sum of interpretable residue-pair and triplet terms, achieves a correlation with experimental binding free energies of R = 0.68 and significantly out-performs other scoring functions. To use our model in protein design applications, we devised a strategy in which synthetic peptides are built by assembling 7-residue native-protein heptad modules into new combinations. An integer linear program was used to find the optimal combination of heptads to bind selectively to a target human bZIP coiled coil, but not to target paralogs. Using this approach, we designed peptides to interact with the bZIP domains from human JUN, XBP1, ATF4 and ATF5. Testing more than 132 candidate protein complexes using a fluorescence resonance energy transfer assay confirmed the formation of tight and selective heterodimers between the designed peptides and their targets. This approach can be used to make inhibitors of native proteins, or to develop novel peptides for applications in synthetic biology or nanotechnology. PMID:25695764

  1. Application of Coiled Coil Peptides in Liposomal Anticancer Drug Delivery Using a Zebrafish Xenograft Model.

    PubMed

    Yang, Jian; Shimada, Yasuhito; Olsthoorn, René C L; Snaar-Jagalska, B Ewa; Spaink, Herman P; Kros, Alexander

    2016-08-23

    The complementary coiled coil forming peptides E4 [(EIAALEK)4] and K4 [(KIAALKE)4] are known to trigger liposomal membrane fusion when tethered to lipid vesicles in the form of lipopeptides. In this study, we examined whether these coiled coil forming peptides can be used for drug delivery applications. First, we prepared E4 peptide modified liposomes containing the far-red fluorescent dye TO-PRO-3 iodide (E4-Lipo-TP3) and confirmed that E4-liposomes could deliver TP3 into HeLa cells expressing K4 peptide on the membrane (HeLa-K) under cell culture conditions in a selective manner. Next, we prepared doxorubicin-containing E4-liposomes (E4-Lipo-DOX) and confirmed that E4-liposomes could also deliver DOX into HeLa-K cells. Moreover, E4-Lipo-DOX showed enhanced cytotoxicity toward HeLa-K cells compared to free doxorubicin. To prove the suitability of E4/K4 coiled coil formation for in vivo drug delivery, we injected E4-Lipo-TP3 or E4-Lipo-DOX into zebrafish xenografts of HeLa-K. As a result, E4-liposomes delivered TP3 to the implanted HeLa-K cells, and E4-Lipo-DOX could suppress cancer proliferation in the xenograft when compared to nontargeted conditions (i.e., zebrafish xenograft with free DOX injection). These data demonstrate that coiled coil formation enables drug selectivity and efficacy in vivo. It is envisaged that these findings are a step forward toward biorthogonal targeting systems as a tool for clinical drug delivery. PMID:27504667

  2. Multiple-Coil, Pulse-Induction Metal Detector

    NASA Technical Reports Server (NTRS)

    Lesky, Edward S.; Reid, Alan M.; Bushong, Wilton E.; Dickey, Duane P.

    1988-01-01

    Multiple-head, pulse-induction metal detector scans area of 72 feet squared with combination of eight detector heads, each 3 ft. square. Head includes large primary coil inducing current in smaller secondary coils. Array of eight heads enables searcher to cover large area quickly. Pulses applied to primary coil, induced in secondary coils measured to determine whether metal present within range of detector head. Detector designed for recovery of Space Shuttle debris.

  3. Leveraging intrinsic chain anisotropy to align coil-coil block copolymers with magnetic fields

    NASA Astrophysics Data System (ADS)

    Rokhlenko, Yekaterina; Zhang, Kai; Gopinadhan, Manesh; Larson, Steve; Majewski, Pawel; Yager, Kevin; Gopalan, Padma; O'Hern, Corey; Osuji, Chinedum

    Magnetic field alignment of block copolymers (BCPs) has typically relied on the presence of liquid crystalline or crystalline assemblies to provide sufficient magnetic anisotropy to drive alignment. Recent experiments however show that alignment is also possible in simple coil-coil BCPs. In particular, alignment of lamellae was observed in poly(styrene-b-4-vinylpyridine) (PS-P4VP) on cooling across the order-disorder transition at field strengths as low as 1 T, with alignment improving markedly with increasing field strength and decreasing cooling rate. Here we discuss the intrinsic chain anisotropy which drives the observed alignment, and its display as a net microdomain anisotropy due to chain tethering at the block interface. We use in-situ X-ray scattering to study the phase behavior and temperature-, time-, and field- dependent dynamics of magnetic alignment in coil-coil BCPs, highlighting the important roles of chain anisotropy and grain size in alignment. For the right combination of field strength and grain size, we can leverage intrinsic chain anisotropy to magnetically direct self-assembly in other coil-coil systems, including cylinder-forming poly(styrene-b-dimethylsiloxane). Field alignment of PS-P4VP with PEO and other blends provides a route to form functional materials such as nanoporous films and ion conducting polymers.

  4. A coiled-coil interaction mediates cauliflower mosaic virus cell-to-cell movement

    PubMed Central

    Stavolone, Livia; Villani, Maria Elena; Leclerc, Denis; Hohn, Thomas

    2005-01-01

    The function of the virion-associated protein (VAP) of cauliflower mosaic virus (CaMV) has long been only poorly understood. VAP is associated with the virion but is dispensable for virus morphogenesis and replication. It mediates virus transmission by aphids through simultaneous interaction with both the aphid transmission factor and the virion. However, although insect transmission is not fundamental to CaMV survival, VAP is indispensable for spreading the virus infection within the host plant. We used a GST pull-down technique to demonstrate that VAP interacts with the viral movement protein through coiled-coil domains and surface plasmon resonance to measure the interaction kinetics. We mapped the movement protein coiled-coil to the C terminus of the protein and proved that it self-assembles as a trimer. Immunogold labeling/electron microscopy revealed that the VAP and viral movement protein colocalize on CaMV particles within plasmodesmata. These results highlight the multifunctional potential of the VAP protein conferred by its efficient coiled-coil interaction system and show a plant virus possessing a surface-exposed protein (VAP) mediating viral entry into host cells. PMID:15837934

  5. A coiled-coil interaction mediates cauliflower mosaic virus cell-to-cell movement

    NASA Astrophysics Data System (ADS)

    Stavolone, Livia; Villani, Maria Elena; Leclerc, Denis; Hohn, Thomas

    2005-04-01

    The function of the virion-associated protein (VAP) of cauliflower mosaic virus (CaMV) has long been only poorly understood. VAP is associated with the virion but is dispensable for virus morphogenesis and replication. It mediates virus transmission by aphids through simultaneous interaction with both the aphid transmission factor and the virion. However, although insect transmission is not fundamental to CaMV survival, VAP is indispensable for spreading the virus infection within the host plant. We used a GST pull-down technique to demonstrate that VAP interacts with the viral movement protein through coiled-coil domains and surface plasmon resonance to measure the interaction kinetics. We mapped the movement protein coiled-coil to the C terminus of the protein and proved that it self-assembles as a trimer. Immunogold labeling/electron microscopy revealed that the VAP and viral movement protein colocalize on CaMV particles within plasmodesmata. These results highlight the multifunctional potential of the VAP protein conferred by its efficient coiled-coil interaction system and show a plant virus possessing a surface-exposed protein (VAP) mediating viral entry into host cells. movement protein | virion-associated protein | Biacore

  6. Open coil structure for bubble-memory-device packaging

    NASA Technical Reports Server (NTRS)

    Chen, T. T.; Ypma, J. E.

    1975-01-01

    Concept has several important advantages over close-wound system: memory and coil chips are separate and interchangeable; interconnections in coil level are eliminated by packing memory chip and electronics in single structure; and coil size can be adjusted to optimum value in terms of power dissipation and field uniformity.

  7. Embolization of Large Aneurysms with Long Wire Coils

    SciTech Connect

    Golzarian, Jafar; Dussaussois, Luc; Ait Said, Kamel; Abada, Hicham T.; Dereume, Jean P.; Struyven, Julien

    2002-01-15

    The authors report the experience of using long coils of 2 m length in the management of large aneurysms. Knowledge of the characteristics of these coils is of value for correct placement. These coils are safe and cost-effective for excluding large aneurysms.

  8. New Retrievable Coil Anchors: Preliminary In Vivo Experiences in Swine

    SciTech Connect

    Konya, A. Wright, K.C.

    2005-04-15

    Purpose. To design and test retrievable coil anchors to improve the safety and efficacy of coil embolization. Methods. Fifty-two 0.038-inch homemade retrievable stainless steel coils were equipped with one of four different pre-shaped nitinol anchors and tested in 38 pigs. All coils with the anchor were completely retrieved and redeployed 3-18 times (median 7 times) prior to release. Types 1 and 2 anchored coils were acutely deployed in the external iliac arteries (n = 10 each), and chronically tested (1 week) in the common carotid arteries (n = 6 each). Larger type 1 (n = 4), type 3 (n = 6), and type 4 (n = 4) anchored coils were acutely deployed in the abdominal aorta. The largest type 1 anchors (n = 6) were acutely tested in the inferior vena cava. Results. All anchored coils were successfully retrieved and repositioned several times. All but two coils formed a compact plug and there was no coil migration except with two mechanically defective type 3 anchors. Conclusion. The use of retrievable anchors allowed the coils to be retrieved and repositioned, prevented coil migration, and enabled compact coil configuration.

  9. Cloning and expression analysis of mouse Cclp1, a new gene encoding a coiled-coil-like protein.

    PubMed

    Noben-Trauth, K; Naggert, J K; Nishina, P M

    1997-05-30

    Here we describe the nucleotide sequence and expression pattern of a novel gene termed Coiled-coil-like protein 1 (Cclp1). A 2646bp open reading frame encodes a 882 amino acid protein with a predicted coiled-coil domain at the amino terminus. Cclp1 is expressed in a variety of adult tissues and during different stages of embryogenesis. The broad expression pattern suggests a general cellular function of CCLP1. PMID:9199242

  10. Domain organization, folding and stability of bacteriophage T4 fibritin, a segmented coiled-coil protein.

    PubMed

    Boudko, Sergei P; Londer, Yuri Y; Letarov, Andrei V; Sernova, Natalia V; Engel, Juergen; Mesyanzhinov, Vadim V

    2002-02-01

    Fibritin is a segmented coiled-coil homotrimer of the 486-residue product of phage T4 gene wac. This protein attaches to a phage particle by the N-terminal region and forms fibrous whiskers of 530 A, which perform a chaperone function during virus assembly. The short C-terminal region has a beta-annulus-like structure. We engineered a set of fibritin deletion mutants sequentially truncated from the N-termini, and the mutants were studied by differential scanning calorimetry (DSC) and CD measurements. The analysis of DSC curves indicates that full-length fibritin exhibits three thermal-heat-absorption peaks centred at 321 K (Delta H=1390 kJ x mol trimer(-1)), at 336 K (Delta H=7600 kJ x mol trimer(-1)), and at 345 K (Delta H=515 kJ x mol trimer(-1)). These transitions were assigned to the N-terminal, segmented coiled-coil, and C-terminal functional domains, respectively. The coiled-coil region, containing 13 segments, melts co-operatively as a single domain with a mean enthalpy Delta Hres=21 kJ x mol residue(-1). The ratio of Delta HVH/Delta Hcal for the coiled-coil part of the 120-, 182-, 258- and 281-residue per monomer mutants, truncated from the N-termini, and for full-length fibritin are 0.91, 0.88, 0.42, 0.39, and 0.13, respectively. This gives an indication of the decrease of the 'all-or-none' character of the transition with increasing protein size. The deletion of the 12-residue-long loop in the 120-residue fibritin increases the thermal stability of the coiled-coil region. According to CD data, full-length fibritin and all the mutants truncated from the N-termini refold properly after heat denaturation. In contrast, fibritin XN, which is deleted for the C-terminal domain, forms aggregates inside the cell. The XN protein can be partially refolded by dilution from urea and does not refold after heat denaturation. These results confirm that the C-terminal domain is essential for correct fibritin assembly both in vivo and in vitro and acts as a foldon. PMID

  11. Subunit b-Dimer of the Escherichia coli ATP Synthase Can Form Left-Handed Coiled-Coils

    PubMed Central

    Wise, John G.; Vogel, Pia D.

    2008-01-01

    One remaining challenge to our understanding of the ATP synthase concerns the dimeric coiled-coil stator subunit b of bacterial synthases. The subunit b-dimer has been implicated in important protein interactions that appear necessary for energy conservation and that may be instrumental in energy conservation during rotary catalysis by the synthase. Understanding the stator structure and its interactions with the rest of the enzyme is crucial to the understanding of the overall catalytic mechanism. Controversy exists on whether subunit b adopts a classic left-handed or a presumed right-handed dimeric coiled-coil and whether or not staggered pairing between nonhomologous residues in the homodimer is required for intersubunit packing. In this study we generated molecular models of the Escherichia coli subunit b-dimer that were based on the well-established heptad-repeat packing exhibited by left-handed, dimeric coiled-coils by employing simulated annealing protocols with structural restraints collected from known structures. In addition, we attempted to create hypothetical right-handed coiled-coil models and left- and right-handed models with staggered packing in the coiled-coil domains. Our analyses suggest that the available structural and biochemical evidence for subunit b can be accommodated by classic left-handed, dimeric coiled-coil quaternary structures. PMID:18326648

  12. CCBuilder: an interactive web-based tool for building, designing and assessing coiled-coil protein assemblies

    PubMed Central

    Wood, Christopher W.; Bruning, Marc; Ibarra, Amaurys Á.; Bartlett, Gail J.; Thomson, Andrew R.; Sessions, Richard B.; Brady, R Leo; Woolfson, Derek N.

    2014-01-01

    Motivation: The ability to accurately model protein structures at the atomistic level underpins efforts to understand protein folding, to engineer natural proteins predictably and to design proteins de novo. Homology-based methods are well established and produce impressive results. However, these are limited to structures presented by and resolved for natural proteins. Addressing this problem more widely and deriving truly ab initio models requires mathematical descriptions for protein folds; the means to decorate these with natural, engineered or de novo sequences; and methods to score the resulting models. Results: We present CCBuilder, a web-based application that tackles the problem for a defined but large class of protein structure, the α-helical coiled coils. CCBuilder generates coiled-coil backbones, builds side chains onto these frameworks and provides a range of metrics to measure the quality of the models. Its straightforward graphical user interface provides broad functionality that allows users to build and assess models, in which helix geometry, coiled-coil architecture and topology and protein sequence can be varied rapidly. We demonstrate the utility of CCBuilder by assembling models for 653 coiled-coil structures from the PDB, which cover >96% of the known coiled-coil types, and by generating models for rarer and de novo coiled-coil structures. Availability and implementation: CCBuilder is freely available, without registration, at http://coiledcoils.chm.bris.ac.uk/app/cc_builder/ Contact: D.N.Woolfson@bristol.ac.uk or Chris.Wood@bristol.ac.uk PMID:25064570

  13. Change in the coil distribution of electrodynamic suspension system

    NASA Technical Reports Server (NTRS)

    Tanaka, Hisashi

    1992-01-01

    At the Miyazaki Maglev Test Center, the initial test runs were completed using a system design that required the superconducting coils to be parallel with the ground levitation coils. Recently, the coil distribution was changed to a system such that the two types of coils were perpendicular to each other. Further system changes will lead to the construction of a side wall levitation system. It is hoped that the development will culminate in a system whereby a superconducting coil will maintain all the functions: levitation, propulsion, and guidance.

  14. Radial and tangential winding coil probes for sextupole magnet measurements

    SciTech Connect

    Kim, S.H.

    1995-08-01

    Rotating coil probes of radial and tangential winding geometries for the measurements of the magnetic center, main field integral and multipole coefficients of sextupole magnets are describes. Two sets of coils are sufficient for a probe of radial winding geometry. For a tangential winding probe, however, typically several sets of coils are required to measure the above magnetic parameters. The tangential coil geometry in this note is described with three sets of coils. The main sextupole field coefficients are defined as b{sub 2} = 1.0cm{sup {minus}2} and a{sub 2} = 0 for the expression of the multipole field coefficients.

  15. SSC (Superconducting Super Collider) dipole coil production tooling

    SciTech Connect

    Carson, J.A.; Barczak, E.J.; Bossert, R.C.; Brandt, J.S.; Smith, G.A.

    1989-03-01

    Superconducting Super Collider dipole coils must be produced to high precision to ensure uniform prestress and even conductor distribution within the collared coil assembly. Tooling is being prepared at Fermilab for the production of high precision 1M and 16.6M SSC dipole coils suitable for mass production. The design and construction methods builds on the Tevatron tooling and production experience. Details of the design and construction methods and measured coil uniformity of 1M coils will be presented. 4 refs., 10 figs.

  16. Magnetically Damped Furnace Bitter Magnet Coil 1

    NASA Technical Reports Server (NTRS)

    Bird, M. D.

    1997-01-01

    A magnet has been built by the National High Magnetic Field Laboratory for NASA on a cost reimbursement contract. The magnet is intended to demonstrate the technology and feasibility of building a magnet for space based crystal growth. A Bitter magnet (named after Francis Bitter, its inventor) was built consisting of four split coils electrically in series and hydraulically in parallel. The coils are housed in a steel vessel to reduce the fringe field and provide some on-axis field enhancement. The steel was nickel plated and Teflon coated to minimize interaction with the water cooling system. The magnet provides 0.14 T in a 184 mm bore with 3 kW of power.

  17. Common Coil Magnet System for VLHC

    SciTech Connect

    Gupta, R.

    1999-02-12

    This paper introduces the common coil magnet system for the proposed very large hadron collider (VLHC). In this system, the high energy booster (HEB), the injector to VLHC, is integrated as the iron dominated low field aperture within the coldmass of the common coil magnet design introduced earlier. This 4-in-1 magnet concept for a 2-in-1 machine should provide a major cost reduction in building and operating VLHC. Moreover, the proposed design reduces the field quality problems associated with the large persistent currents in Nb{sub 3}Sn magnets. The paper also shows that the geometric field harmonics can be made small. In this preliminary magnetic design. the current dependence in harmonics is significant but not umnanageable.

  18. Choice of coils for a fusion reactor

    PubMed Central

    Alexander, Romeo; Garabedian, Paul R.

    2007-01-01

    In a fusion reactor a hot plasma of deuterium and tritium is confined by a strong magnetic field to produce helium ions and release energetic neutrons. The 3D geometry of a stellarator provides configurations for such a device that reduce net toroidal current that might lead to disruptions. We construct smooth coils generating an external magnetic field designed to prevent the plasma from deteriorating. PMID:17640879

  19. Acute lung injury following refrigeration coil deicing.

    PubMed

    McKeown, Nathanael J; Burton, Brent T

    2012-03-01

    We report a case of a worker who developed ALI requiring mechanical ventilatory support after attempting to melt ice condensate by applying the flame of an oxy-acetylene torch to refrigeration coils charged with a halocarbon refrigerant in a closed environment. A discussion of possible etiologies are discussed, including phosgene, carbonyl fluoride, and nitrogen oxides. Primary prevention with adequate respiratory protection is recommended whenever deicing is performed in a closed space environment. PMID:22372791

  20. Choice of coils for a fusion reactor.

    PubMed

    Alexander, Romeo; Garabedian, Paul R

    2007-07-24

    In a fusion reactor a hot plasma of deuterium and tritium is confined by a strong magnetic field to produce helium ions and release energetic neutrons. The 3D geometry of a stellarator provides configurations for such a device that reduce net toroidal current that might lead to disruptions. We construct smooth coils generating an external magnetic field designed to prevent the plasma from deteriorating. PMID:17640879

  1. Stress relaxation in SSC 50mm dipole coils

    SciTech Connect

    Rogers, D.; Markley, F.

    1992-04-01

    We are measuring the stress relaxation of SSC 50mm outer coils with the goal of predicting how much of the coil prestress will be lost while the coils are warehoused between manufacture and cooldown. We manufacture 3 inch (76.2mm) long segments of coil with the same materials and techniques that have been used for prototype coils. We are running four simultaneous tests in an attempt to separate the contributions of the different coil materials. Test one is a completely insulated coil section where the insulation is the all polyamide system being tested at Brookhaven; test two is a wire stack insulated only with the normal Kapton overwrap; test three is a stack of bare cable; and test four is a completely insulated normal coil section. All, except for the bare cable, include the ground insulation. The insulated coil sections are carefully dried before loading and testing in order to eliminate stress changes due to varying moisture content. The temperature dependence of the stress relaxation is being studied separately. Three companion papers presented at this conference will be: (1) Temperature dependence of the viscoelastic properties of SSC coil insulation'' (2) Measurement of the elastic modulus of Kapton perpendicular to the plane of the film at room and cryogenic temperatures'' (3) Theoretical methods for creep and stress relaxation studies of SSC coil.''

  2. Deep brain transcranial magnetic stimulation using variable "Halo coil" system

    NASA Astrophysics Data System (ADS)

    Meng, Y.; Hadimani, R. L.; Crowther, L. J.; Xu, Z.; Qu, J.; Jiles, D. C.

    2015-05-01

    Transcranial Magnetic Stimulation has the potential to treat various neurological disorders non-invasively and safely. The "Halo coil" configuration can stimulate deeper regions of the brain with lower surface to deep-brain field ratio compared to other coil configurations. The existing "Halo coil" configuration is fixed and is limited in varying the site of stimulation in the brain. We have developed a new system based on the current "Halo coil" design along with a graphical user interface system that enables the larger coil to rotate along the transverse plane. The new system can also enable vertical movement of larger coil. Thus, this adjustable "Halo coil" configuration can stimulate different regions of the brain by adjusting the position and orientation of the larger coil on the head. We have calculated magnetic and electric fields inside a MRI-derived heterogeneous head model for various positions and orientations of the coil. We have also investigated the mechanical and thermal stability of the adjustable "Halo coil" configuration for various positions and orientations of the coil to ensure safe operation of the system.

  3. FIRST 100 T NON-DESTRUCTIVE MAGNET OUTER COIL SET

    SciTech Connect

    J. BACON; A. BACA; ET AL

    1999-09-01

    The controlled power outer coil set of the first 100 T non-destructive (100 T ND) magnet is described. This magnet will be installed as part of the user facility research equipment at the National High Magnetic Field laboratory (NHMFL) Pulsed Field Facility at Los Alamos National Laboratory. The 100 T ND controlled power outer coil set consists of seven nested, mechanically independent externally reinforced coils. These coils, in combination, will produce a 47 T platform field in a 225-mm diameter bore. Using inertial energy storage a synchronous motor/generator provides ac power to a set of seven ac-dc converters rated at 64 MW/80 MVA each. These converters energize three independent coil circuits to create 170 MJ of field energy in the outer coil set at the platform field of 47 T. Each coil consists of a multi-layer winding of high strength conductor supported by an external high strength stainless steel shell. Coils with the highest magnetic loads will utilize a reinforcing shell fabricated from highly cold worked 301 stainless steel strip. The autofrettage conditioning method will be used to pre-stress the coils and thereby limit conductor and reinforcement strains to the elastic range. The purpose of pre-stressing the coils is to attain a design life of 10,000 full field pulses. The operation and conditioning of the coil set will be described along with special features of its design, magnetic and structural analyses and construction.

  4. Practical Feasibility and Packing Density of Endovascular Coiling Using Target® Nano™ Coils in Small Cerebral Aneurysms

    PubMed Central

    Jeong, Hae Woong

    2015-01-01

    Objective Based on the use of Nano™ coils, we retrospectively compared the proportion of the coils (≤ 1.5 mm) and packing density in two patient groups with small cerebral aneurysms (< 4 mm diameter) who were treated with or without Nano™ coils. Materials and Methods Between January 2012 and November 2013, in 548 cerebral aneurysms treated by endovascular coiling, 143 patients with 148 small cerebral aneurysms underwent endovascular coiling. After March 2013, coiling with Nano™ coils was performed on 45 small cerebral aneurysms (30.4%). Results There were no significant differences in the size and locations of the cerebral aneurysms, the age of the patients, and the procedural modalities between the two groups. The proportion of the coil (≤ 1.5 mm) of the group treated with Nano™ coils (53.6%) was higher than the proportion of the coil (≤ 1.5 mm) of the group treated without Nano™ coils (14.7%) with statistical significance (p < 0.001). The packing density of the group treated with Nano™ coils (31.3 ± 9.69%) was higher than the packing density of the group treated without Nano™ coils (29.49 ± 7.84%), although the difference was not significant. Procedural complications developed in 3 lesions (2 thromboembolisms and 1 carotid dissection) (2.0%). Treatment-related transient neurological deficits due to thromboembolism developed in 1 lesion, which had not been treated with Nano™ coils. There was no treatment-related permanent morbidity or mortality in either of the groups. Conclusion In our series, the small cerebral aneurysms treated with Nano™ coils showed more packing density with no additive procedural risk or difficulty. PMID:27064999

  5. Endovascular coil trapping of a ruptured dissecting aneurysm of the vertebral artery using detachable coils and micro-tornado® coils.

    PubMed

    Kim, Myeong-Soo

    2013-06-01

    We experienced a patient with a ruptured dissecting aneurysm of the vertebral artery who was treated by trapping of the lesion using Guglielmi detachable coils (GDCs) with micro-tornado® coils (MTCs). An 80-year-old male was transferred with a ruptured left vertebral artery dissecting aneurysm (VADA). The dissected portion of the vertebral artery was effectively trapped using GDCs and MTCs. The MTCs used for neurointervention were comprised of various types of coils and we successfully placed them into the parent artery of the dissected segment. The author suggests that this case demonstrates the usefulness of endovascular coil trapping of VADAs using MTCs in achievement of embolization. PMID:23844353

  6. Endovascular Coil Trapping of a Ruptured Dissecting Aneurysm of the Vertebral Artery Using Detachable Coils and Micro-Tornado® Coils

    PubMed Central

    2013-01-01

    We experienced a patient with a ruptured dissecting aneurysm of the vertebral artery who was treated by trapping of the lesion using Guglielmi detachable coils (GDCs) with micro-tornado® coils (MTCs). An 80-year-old male was transferred with a ruptured left vertebral artery dissecting aneurysm (VADA). The dissected portion of the vertebral artery was effectively trapped using GDCs and MTCs. The MTCs used for neurointervention were comprised of various types of coils and we successfully placed them into the parent artery of the dissected segment. The author suggests that this case demonstrates the usefulness of endovascular coil trapping of VADAs using MTCs in achievement of embolization. PMID:23844353

  7. In situ calibration of rotating sensor coils for magnet testing

    SciTech Connect

    Arpaia, P.; Golluccio, G.; Buzio, M.; Walckiers, L.

    2012-01-15

    An in situ procedure for calibrating equivalent magnetic area and rotation radius of rotating coils is proposed for testing accelerator magnets shorter than the measuring coil. The procedure exploits measurements of magnetic field and mechanical displacement inside a reference quadrupole magnet. In a quadrupole field, an offset between the magnet and coil rotation axes gives rise to a dipole component in the field series expansion. The measurements of the focusing strength, the displacement, and the resulting dipole term allow the equivalent area and radius of the coil to be determined analytically. The procedure improves the accuracy of coils with large geometrical irregularities in the winding. This is essential for short magnets where the coil dimensions constrain the measurement accuracy. Experimental results on different coils measuring small-aperture permanent magnets are shown.

  8. Application & testing of high temperature materials for solenoid coils

    SciTech Connect

    Sanchez, R.O.; Archer, W.E.; Zich, J.L.

    1997-08-01

    Sandia National Laboratories has designed and proven-in two new Solenoid coils for a highly-reliable electromechanical switch. Mil-Spec Magnetics Inc., Walnut CA manufactured the coils. The new design utilizes two new materials: Liquid Crystal Polymer (Vectra C130) for the bobbin and Thermal Barrier Silicone (VI-SIL V-658) for the encapsulant. The use of these two new materials solved most of the manufacturing problems inherent in the old Sandia design. The coils are easier to precision wind and more robust for handling, testing, and storage. The coils have some unique weapon related safety requirements. The most severe of these requirements is the 400{degrees}C, 1600 V test. The coils must not, and did not, produce any outgassing products to affect the voltage breakdown between contacts in the switch at these temperatures and voltages. Actual coils in switches were tested under these conditions. This paper covers the prove-in of this new coil design.

  9. Carbon footprint of automotive ignition coil

    NASA Astrophysics Data System (ADS)

    Chang, Huey-Ling; Chen, Chih-Ming; Sun, Chin-Huang; Lin, Hung-Di

    2015-07-01

    In recent years, environmental issues, such as climate change and global warming due to the excessive development of industry, have attracted increasing attention of citizens worldwide. It is known that CO2 accounts for the largest proportion of greenhouse gases. Therefore, how to reduce CO2 emissions during the life cycle of a product to lessen its impact on environment is an important topic in the industrial society. Furthermore, it is also of great significance to cut down the required energy so as to lower its production costs during the manufacturing process nowadays. This study presents the carbon footprint of an automotive ignition coil and its partial materials are defined to explore their carbon emissions and environmental impact. The model IPCC GWP100a calculates potential global greenhouse effect by converting them into CO2 equivalents. In this way, the overall carbon footprint of an ignition coil can be explored. By using IPCC GWP100a, the results display that the shell has the most carbon emissions. The results can help the industry reduce the carbon emissions of an ignition coil product.

  10. Throttle coil operation of TMX-U

    SciTech Connect

    Correll, D.L.; Byers, J.A.; Casper, T.A.

    1983-01-01

    A tandem-mirror configuration with an axisymmetric central cell, similar to the geometry of MARS (Mirror Advanced Reactor Study) or the Kelley-TDF mode of MFTB-B, can be generated by inserting a 6-tesla, throttle coil in each end of the TMX-U central cell. The throttle coil geometry of TMX-U will test the physics issues associated with axisymmetric tandem-mirror reactors, such issues as: (1) increased radial confinement time for central-cell ions confined by axisymmetric mirror cells and electrostatic potentials; (2) theoretical limits set by the trapped particle instability for the required passing density between the central cell and the end-cell anchor; and (3) pumping of trapped particles within the thermal barrier and transition regions with methods other than neutral beams. The central-cell plasma parameters for the throttle coil geometry are evaluated for two operating points. The first requires heating hardware (neutral beams and ECRH) and vacuum performance at the TMX-U proposal level, yielding plasma parameters, central-cell betas, and plasma confinement exceeding those of the original TMX-U proposal. The second operating point, requiring approximately half the ECRH end-cell performance of the first, is predicted to equal the beta and to exceed the plasma pressure and confinement time of the central cell in the standard TMX-U geometry.

  11. Numerical dosimetry of transcranial magnetic stimulation coils

    NASA Astrophysics Data System (ADS)

    Crowther, Lawrence; Hadimani, Ravi; Jiles, David

    2014-03-01

    Transcranial magnetic stimulation (TMS) is a non-invasive neuromodulation technique capable of stimulating neurons by means of electromagnetic induction. TMS can be used to map brain function and shows promise for the diagnosis and treatment of neurological and psychiatric disorders. Calculation of fields induced in the brain are necessary to accurately identify stimulated neural tissue during TMS. This allows the development of novel TMS coil designs capable of stimulating deeper brain regions and increasing the localization of stimulation that can be achieved. We have performed numerical calculations of magnetic and electric field with high-resolution anatomically realistic human head models to find these stimulated brain regions for a variety of proposed TMS coil designs. The realistic head models contain heterogeneous tissue structures and electrical conductivities, yielding superior results to those obtained from the simplified homogeneous head models that are commonly employed. The attenuation of electric field as a function of depth in the brain and the localization of stimulating field have been methodically investigated. In addition to providing a quantitative comparison of different TMS coil designs the variation of induced field between subjects has been investigated. We also show the differences in induced fields between adult, adolescent and child head models to preemptively identify potential safety issues in the application of pediatric TMS.

  12. Mechanical resonances of helically coiled carbon nanowires.

    PubMed

    Saini, D; Behlow, H; Podila, R; Dickel, D; Pillai, B; Skove, M J; Serkiz, S M; Rao, A M

    2014-01-01

    Despite their wide spread applications, the mechanical behavior of helically coiled structures has evaded an accurate understanding at any length scale (nano to macro) mainly due to their geometrical complexity. The advent of helically coiled micro/nanoscale structures in nano-robotics, nano-inductors, and impact protection coatings has necessitated the development of new methodologies for determining their shear and tensile properties. Accordingly, we developed a synergistic protocol which (i) integrates analytical, numerical (i.e., finite element using COMSOL) and experimental (harmonic detection of resonance; HDR) methods to obtain an empirically validated closed form expression for the shear modulus and resonance frequency of a singly clamped helically coiled carbon nanowire (HCNW), and (ii) circumvents the need for solving 12th order differential equations. From the experimental standpoint, a visual detection of resonances (using in situ scanning electron microscopy) combined with HDR revealed intriguing non-planar resonance modes at much lower driving forces relative to those needed for linear carbon nanotube cantilevers. Interestingly, despite the presence of mechanical and geometrical nonlinearities in the HCNW resonance behavior the ratio of the first two transverse modes f₂/f₁ was found to be similar to the ratio predicted by the Euler-Bernoulli theorem for linear cantilevers. PMID:24986377

  13. Single coil bistable, bidirectional micromechanical actuator

    DOEpatents

    Tabat, Ned; Guckel, Henry

    1998-09-15

    Micromechanical actuators capable of bidirectional and bistable operation can be formed on substrates using lithographic processing techniques. Bistable operation of the microactuator is obtained using a single coil and a magnetic core with a gap. A plunger having two magnetic heads is supported for back and forth linear movement with respect to the gap in the magnetic core, and is spring biased to a neutral position in which the two heads are on each side of the gap in the core. The single electrical coil is coupled to the core and is provided with electrical current to attract one of the heads toward the core by reluctance action to drive the plunger to a limit of travel in one direction. The current is then cut off and the plunger returns by spring action toward the gap, whereafter the current is reapplied to the coil to attract the other head of the plunger by reluctance action to drive the plunger to its other limit of travel. This process can be repeated at a time when switching of the actuator is required.

  14. Coiling of elastic rods on rigid substrates.

    PubMed

    Jawed, Mohammad K; Da, Fang; Joo, Jungseock; Grinspun, Eitan; Reis, Pedro M

    2014-10-14

    We investigate the deployment of a thin elastic rod onto a rigid substrate and study the resulting coiling patterns. In our approach, we combine precision model experiments, scaling analyses, and computer simulations toward developing predictive understanding of the coiling process. Both cases of deposition onto static and moving substrates are considered. We construct phase diagrams for the possible coiling patterns and characterize them as a function of the geometric and material properties of the rod, as well as the height and relative speeds of deployment. The modes selected and their characteristic length scales are found to arise from a complex interplay between gravitational, bending, and twisting energies of the rod, coupled to the geometric nonlinearities intrinsic to the large deformations. We give particular emphasis to the first sinusoidal mode of instability, which we find to be consistent with a Hopf bifurcation, and analyze the meandering wavelength and amplitude. Throughout, we systematically vary natural curvature of the rod as a control parameter, which has a qualitative and quantitative effect on the pattern formation, above a critical value that we determine. The universality conferred by the prominent role of geometry in the deformation modes of the rod suggests using the gained understanding as design guidelines, in the original applications that motivated the study. PMID:25267649

  15. Coiling and Folding of Viscoelastic Jets

    NASA Astrophysics Data System (ADS)

    Majmudar, Trushant; Varagnat, Matthieu; McKinley, Gareth

    2007-11-01

    The study of fluid jets impacting on a flat surface has industrial applications in many areas, including processing of foods and consumer goods, bottle filling, and polymer melt processing. Previous studies have focused primarily on purely viscous, Newtonian fluids, which exhibit a number of different dynamical regimes including dripping, steady jetting, folding, and steady coiling. Here we add another dimension to the problem by focusing on mobile (low viscosity) viscoelastic fluids, with the study of two wormlike-micellar fluids, a cetylpyridinum-salicylic acid salt (CPyCl/NaSal) solution, and an industrially relevant shampoo base. We investigate the effects of viscosity and elasticity on the dynamics of axi-symmetric jets. The viscoelasticity of the fluids is systematically controlled by varying the concentration of salt counterions. Experimental methods include shear and extensional rheology measurements to characterize the fluids, and high-speed digital video imaging. In addition to the regimes observed in purely viscous systems, we also find a novel regime in which the elastic jet buckles and folds on itself, and alternates between coiling and folding behavior. We suggest phase diagrams and scaling laws for the coiling and folding frequencies through a systematic exploration of the experimental parameter space (height of fall, imposed flow rate, elasticity of the solution).

  16. Designing HTS coils for magnetic circuits

    SciTech Connect

    Jenkins, R.G.; Jones, H.; Goodall, R.M.

    1996-07-01

    The authors discuss some of the main considerations involved in the design of HTS coils to operate in liquid nitrogen and provide ampere-turns for magnetic circuits in general, and then in particular for a small-scale electromagnetic (i.e, attractive) maglev demonstrator. The most important factor affecting design is the sensitive and strongly anisotropic dependence of HTS tape`s critical current on magnetic field. Any successful design must limit the field in the windings, especially components perpendicular to the tape`s surface (radial components in the case of solenoids), to acceptably low levels such that local critical currents nowhere fall below the operating current. This factor is relevant to the construction of HTS coils for all applications. A second important factor is that the presence of an iron magnetic circuit can greatly alter the flux distribution within the coils from that found when they are in free space. FE modelling has been used to calculate accurate field profiles in proposed designs for comparison with short sample I{sub c}(B) data. They present a design for a maglev demonstrator, illustrating how some of the problems, in particular the reduction of radial field components, may be addressed, and describe its predicted performance.

  17. Electrodynamic Maglev coil design and analysis

    SciTech Connect

    Davey, K.R.

    1997-09-01

    Electrodynamic Maglev systems are distinguished from electromagnetic systems in that the currents yielding lift and guidance forces are induced by the movement of the vehicle. Above a threshold speed, such a system is inherently stable, and has the additional benefit of having greater flexibility to system construction tolerances in that the magnet vertical position can change by as much as 5 cm. A stacked magnet design is considered which couples into a set of interleave coils which are interconnected in such a way as to yield both lift and guidance forces. A mutual coupling analysis is embraced wherein the mutual inductance between the permanent magnets on board the vehicle and the coils are computed using closed form analytical expressions for filaments. The derivatives of these expressions are then averaged to compute both the induced current and the forces on the coils as a function of the system geometry and speed. A full transient analysis must be performed to accurately account for entry and exit effects. The results are compared to those experimentally measured on a test track and extrapolations are offered to suggest future design considerations.

  18. Coiling of elastic rods on rigid substrates

    PubMed Central

    Jawed, Mohammad K.; Da, Fang; Joo, Jungseock; Grinspun, Eitan; Reis, Pedro M.

    2014-01-01

    We investigate the deployment of a thin elastic rod onto a rigid substrate and study the resulting coiling patterns. In our approach, we combine precision model experiments, scaling analyses, and computer simulations toward developing predictive understanding of the coiling process. Both cases of deposition onto static and moving substrates are considered. We construct phase diagrams for the possible coiling patterns and characterize them as a function of the geometric and material properties of the rod, as well as the height and relative speeds of deployment. The modes selected and their characteristic length scales are found to arise from a complex interplay between gravitational, bending, and twisting energies of the rod, coupled to the geometric nonlinearities intrinsic to the large deformations. We give particular emphasis to the first sinusoidal mode of instability, which we find to be consistent with a Hopf bifurcation, and analyze the meandering wavelength and amplitude. Throughout, we systematically vary natural curvature of the rod as a control parameter, which has a qualitative and quantitative effect on the pattern formation, above a critical value that we determine. The universality conferred by the prominent role of geometry in the deformation modes of the rod suggests using the gained understanding as design guidelines, in the original applications that motivated the study. PMID:25267649

  19. Mechanical Resonances of Helically Coiled Carbon Nanowires

    NASA Astrophysics Data System (ADS)

    Saini, D.; Behlow, H.; Podila, R.; Dickel, D.; Pillai, B.; Skove, M. J.; Serkiz, S. M.; Rao, A. M.

    2014-07-01

    Despite their wide spread applications, the mechanical behavior of helically coiled structures has evaded an accurate understanding at any length scale (nano to macro) mainly due to their geometrical complexity. The advent of helically coiled micro/nanoscale structures in nano-robotics, nano-inductors, and impact protection coatings has necessitated the development of new methodologies for determining their shear and tensile properties. Accordingly, we developed a synergistic protocol which (i) integrates analytical, numerical (i.e., finite element using COMSOL®) and experimental (harmonic detection of resonance; HDR) methods to obtain an empirically validated closed form expression for the shear modulus and resonance frequency of a singly clamped helically coiled carbon nanowire (HCNW), and (ii) circumvents the need for solving 12th order differential equations. From the experimental standpoint, a visual detection of resonances (using in situ scanning electron microscopy) combined with HDR revealed intriguing non-planar resonance modes at much lower driving forces relative to those needed for linear carbon nanotube cantilevers. Interestingly, despite the presence of mechanical and geometrical nonlinearities in the HCNW resonance behavior the ratio of the first two transverse modes f2/f1 was found to be similar to the ratio predicted by the Euler-Bernoulli theorem for linear cantilevers.

  20. Evidence of α-helical coiled coils and β-sheets in hornet silk.

    PubMed

    Kameda, Tsunenori; Nemoto, Takashi; Ogawa, Tetsuya; Tosaka, Masatoshi; Kurata, Hiroki; Schaper, Andreas K

    2014-03-01

    α-Helical coiled coil and β-sheet complexes are essential structural building elements of silk proteins produced by different species of the Hymenoptera. Beside X-ray scattering at wide and small angles we applied cryo-electron diffraction and microscopy to demonstrate the presence and the details of such structures in silk of the giant hornet Vespa mandarinia japonica. Our studies on the assembly of the fibrous silk proteins and their internal organization in relation to the primary chain structure suggest a 172 Å pitch supercoil consisting of four intertwined alanine-rich α-helical strands. The axial periodicity may adopt even multiples of the pitch value. Coiled coil motifs form the largest portion of the hornet silk structure and are aligned nearly parallel to the cocoon fiber axis in the same way as the membrane-like parts of the cocoon are molecularly orientated in the spinning direction. Supercoils were found to be associated with β-crystals, predominantly localized in the l-serine-rich chain sequences terminating each of the four predominant silk proteins. Such β-sheet blocks are considered resulting from transformation of random coil molecular sequences due to the action of elongational forces during the spinning process. PMID:24345346

  1. Theoretical design of gradient coils with minimum power dissipation: accounting for the discretization of current density into coil windings.

    PubMed

    While, Peter T; Korvink, Jan G; Shah, N Jon; Poole, Michael S

    2013-10-01

    Gradient coil windings are typically constructed from either variable width copper tracks or fixed width wires. Excessive power dissipation within these windings during gradient coil operation limits the maximum drive current or duty cycle of the coil. It is common to design gradient coils in terms of a continuous minimum power current density and to perform a discretization to obtain the locations of the coil tracks or wires. However, the existence of finite gaps between these conductors and a maximum conductor width leads to an underestimation of coil resistance when calculated using the continuous current density. Put equivalently, the actual current density within the tracks or wires is higher than that used in the optimization and this departure results in suboptimal coil designs. In this work, a mapping to an effective current density is proposed to account for these effects and provide the correct contribution to the power dissipation. This enables the design of gradient coils that are genuinely optimal in terms of power minimization, post-discretization. The method was applied to the theoretical design of a variety of small x- and z-gradient coils for use in small animal imaging and coils for human head imaging. Computer-driven comparisons were made between coils designed with and without the current density mapping, in terms of simulated power dissipation. For coils to be built using variable width tracks, the method provides slight reductions in power dissipation in most cases and substantial gains only in cases where the minimum separation between track centre-lines is less than twice the gap size. However, for coils to be built using fixed width wires, very considerable reductions in dissipated power are consistently attainable (up to 60%) when compared to standard approaches of coil optimization. PMID:23994605

  2. Protein-based hydrogels self-assembled from genetically engineered triblock polypeptides containing coiled-coil domains

    NASA Astrophysics Data System (ADS)

    Xu, Chunyu

    Protein-based biomaterials have great potential in biomedical applications due to their similar composition with biological organisms. Environment-sensitive hydrogels based on proteins can undergo sol-gel transition due to the conformational change of the proteins in response to external stimuli. The physical properties of these hydrogels can be tailored by modification of the protein structures. Two major hypotheses were made in this dissertation. One was that coiled-coil folding motifs could be a good candidate for physical crosslinking in protein-based hydrogels, and the other was that the conformational change of coiled-coils in response to external stimuli could mediate the sol-gel transition of the protein-based hydrogels. The first part established synthesis strategies of the coiled-coil containing proteins using a genetic engineering technique. An important observation was made that the fusion sequence on the proteins could influence the thermal stability of the proteins. In the second part of the research, the self-assembly of hydrogels from a series of triblock polypeptides containing coiled-coils was evaluated. It was found that the hydrogels had a porous interconnected network microstructure. The hydrogels responded to temperature and pH, which correlated to the temperature- and pH-triggered structural transition of the coiled-coil domains. In addition, the formation of hydrogels was reversible in the present or absence of guanidine hydrochloride (GdnHCl). The last part of the research attempted to explore the relationship between the structure of the protein polymers and the physical property of the hydrogels, and to investigate the parameters influencing the hydrogel formation and physical properties. Triblock and diblock polypeptides were designed to contain different lengths of coiled-coil domains. Tyrosine residues were incorporated at selected solvent-exposed positions in order to increase the hydrophobicity of the coiled-coil domains. The

  3. Ablation dynamics in coiled wire-array Z-pinches

    SciTech Connect

    Hall, G. N.; Lebedev, S. V.; Suzuki-Vidal, F.; Swadling, G.; Chittenden, J. P.; Bland, S. N.; Harvey-Thompson, A.; Knapp, P. F.; Blesener, I. C.; McBride, R. D.; Chalenski, D. A.; Blesener, K. S.; Greenly, J. B.; Pikuz, S. A.; Shelkovenko, T. A.; Hammer, D. A.; Kusse, B. R.

    2013-02-15

    Experiments to study the ablation dynamics of coiled wire arrays were performed on the MAGPIE generator (1 MA, 240 ns) at Imperial College, and on the COBRA generator at Cornell University's Laboratory of Plasma Studies (1 MA, 100 ns). The MAGPIE generator was used to drive coiled wires in an inverse array configuration to study the distribution of ablated plasma. Using interferometry to study the plasma distribution during the ablation phase, absolute quantitative measurements of electron line density demonstrated very high density contrasts between coiled ablation streams and inter-stream regions many millimetres from the wire. The measured density contrasts for a coiled array were many times greater than that observed for a conventional array with straight wires, indicating that a much greater axial modulation of the ablated plasma may be responsible for the unique implosion dynamics of coiled arrays. Experiments on the COBRA generator were used to study the complex redirection of plasma around a coiled wire that gives rise to the ablation structure exhibited by coiled arrays. Observations of this complex 3D plasma structure were used to validate the current model of coiled array ablation dynamics [Hall et al., Phys. Rev. Lett. 100, 065003 (2008)], demonstrating irrefutably that plasma flow from the wires behaves as predicted. Coiled wires were observed to ablate and implode in the same manner on both machines, indicating that current rise time should not be an issue for the scaling of coiled arrays to larger machines with fast current rise times.

  4. Quench simulation of SMES consisting of some superconducting coils

    NASA Astrophysics Data System (ADS)

    Noguchi, S.; Oga, Y.; Igarashi, H.

    2011-11-01

    In recent years, many HTS superconducting magnetic energy storage (HTS-SMES) systems are investigated and designed. They usually consist of some superconducting element coils due to storing excessively high energy. If one of them was quenched, the storage energy of the superconducting element coil quenched has to be immediately dispersed to protect the HTS-SMES system. As the result, the current of the other element coils, which do not reach to quench, increases since the magnetic coupling between the quenched element coil and the others are excessively strong. The increase of the current may cause the quench of the other element coils. If the energy dispersion of the element coil quenched was failed, the other superconducting element coil would be quenched in series. Therefore, it is necessary to investigate the behavior of the HTS-SMES after quenching one or more element coils. To protect a chain of quenches, it is also important to investigate the time constant of the coils. We have developed a simulation code to investigate the behavior of the HTS-SMES. By the quench simulation, it is indicated that a chain of quenches is caused by a quench of one element coil.

  5. Ablation dynamics in coiled wire-array Z-pinches

    NASA Astrophysics Data System (ADS)

    Hall, G. N.; Lebedev, S. V.; Suzuki-Vidal, F.; Swadling, G.; Chittenden, J. P.; Bland, S. N.; Harvey-Thompson, A.; Knapp, P. F.; Blesener, I. C.; McBride, R. D.; Chalenski, D. A.; Blesener, K. S.; Greenly, J. B.; Pikuz, S. A.; Shelkovenko, T. A.; Hammer, D. A.; Kusse, B. R.

    2013-02-01

    Experiments to study the ablation dynamics of coiled wire arrays were performed on the MAGPIE generator (1 MA, 240 ns) at Imperial College, and on the COBRA generator at Cornell University's Laboratory of Plasma Studies (1 MA, 100 ns). The MAGPIE generator was used to drive coiled wires in an inverse array configuration to study the distribution of ablated plasma. Using interferometry to study the plasma distribution during the ablation phase, absolute quantitative measurements of electron line density demonstrated very high density contrasts between coiled ablation streams and inter-stream regions many millimetres from the wire. The measured density contrasts for a coiled array were many times greater than that observed for a conventional array with straight wires, indicating that a much greater axial modulation of the ablated plasma may be responsible for the unique implosion dynamics of coiled arrays. Experiments on the COBRA generator were used to study the complex redirection of plasma around a coiled wire that gives rise to the ablation structure exhibited by coiled arrays. Observations of this complex 3D plasma structure were used to validate the current model of coiled array ablation dynamics [Hall et al., Phys. Rev. Lett. 100, 065003 (2008)], demonstrating irrefutably that plasma flow from the wires behaves as predicted. Coiled wires were observed to ablate and implode in the same manner on both machines, indicating that current rise time should not be an issue for the scaling of coiled arrays to larger machines with fast current rise times.

  6. Coil Design Considerations for Deep Transcranial Magnetic Stimulation

    PubMed Central

    Deng, Zhi-De; Lisanby, Sarah H.; Peterchev, Angel V.

    2014-01-01

    Objectives To explore the field characteristics and design tradeoffs of coils for deep transcranial magnetic stimulation (dTMS). Methods We simulated parametrically two dTMS coil designs on a spherical head model using the finite element method, and compare them with five commercial TMS coils, including two that are FDA approved for the treatment of depression (ferromagnetic-core figure-8 and H1 coil). Results Smaller coils have a focality advantage over larger coils; however, this advantage diminishes with increasing target depth. Smaller coils have the disadvantage of producing stronger field in the superficial cortex and requiring more energy. When the coil dimensions are large relative to the head size, the electric field decay in depth becomes linear, indicating that, at best, the electric field attenuation is directly proportional to the depth of the target. Ferromagnetic cores improve electrical efficiency for targeting superficial brain areas; however magnetic saturation reduces the effectiveness of the core for deeper targets, especially for highly focal coils. Distancing winding segments from the head, as in the H1 coil, increases the required stimulation energy. Conclusions Among standard commercial coils, the double cone coil offers high energy efficiency and balance between stimulated volume and superficial field strength. Direct TMS of targets at depths of ~ 4 cm or more results in superficial stimulation strength that exceeds the upper limit in current rTMS safety guidelines. Approaching depths of ~ 6 cm is almost certainly unsafe considering the excessive superficial stimulation strength and activated brain volume. Significance Coil design limitations and tradeoffs are important for rational and safe exploration of dTMS. PMID:24411523

  7. Type I macrophage scavenger receptor contains α-helical and collagen-like coiled coils

    NASA Astrophysics Data System (ADS)

    Kodama, Tatsuhiko; Freeman, Mason; Rohrer, Lucia; Zabrecky, James; Matsudaira, Paul; Krieger, Monty

    1990-02-01

    The macrophage scavenger receptor is a trimeric membrane glycoprotein with unusual ligand-binding properties which has been implicated in the development of atherosclerosis. The trimeric structure of the bovine type I scavenger receptor, deduced by complementary DNA cloning, contains three extracellular C-terminal cysteine-rich domains connected to the transmembrane domain by a long fibrous stalk. This stalk structure, composed of an a-helical coiled coil and a collagen-like triple helix, has not previously been observed in an integral membrane protein.

  8. Effect of chain length on the formation and stability of synthetic alpha-helical coiled coils.

    PubMed

    Su, J Y; Hodges, R S; Kay, C M

    1994-12-27

    A series of polypeptides containing 9, 12, 16, 19, 23, 26, 30, 33, and 35 amino acid residues was designed to investigate the effects of peptide chain length on the formation and stability of two-stranded alpha-helical dimers or coiled coils. These peptides were synthesized by the solid-phase method, purified by reversed-phase high-performance liquid chromatography (RP-HPLC), and characterized by RP-HPLC, amino acid composition analysis, and mass spectrometry. The amphipathic alpha-helical peptides were designed to dimerize by interchain hydrophobic interactions at positions a and d and interchain salt bridges between lysine and glutamic acid residues at positions e and g of the repeating heptad sequence of Glu-Ile-Glu-Ala-Leu-Lys-Ala (g-a-b-c-d-e-f). The ability of these peptides to form alpha-helical structures in the presence and absence of a helix-inducing reagent (trifluoroethanol) was monitored by circular dichroism spectroscopy. The helicity of the peptides increased with increasing chain length in a cooperative manner. A minimum of three heptads corresponding to six helical turns was required for a peptide to adopt the two-stranded alpha-helical coiled coil conformation in aqueous medium. The increased stability of the peptides as a result of an increase in hydrophobic interactions (chain length) was demonstrated by the shift in the transitions of the guanidine hydrochloride (Gdn.HCl) denaturation and thermal unfolding profiles. The concentrations of denaturant (Gdn.HCl) required to achieve 50% denaturation are 3.2, 4.9, 6.9, and 7.5 M for peptides 23r, 26r, 30r, and 33r, respectively, in aqueous medium. However, the effect of a chain length increase on coiled-coil stability was not additive. The melting temperature, Tm, at which 50% of the helicity is lost, increased by 34 degrees C in changing the peptide chain length from 23 to 26; however, that shift was only 14 degrees C when the chain length was increased from 30 to 33 residues. These results are

  9. Protein destabilization by electrostatic repulsions in the two-stranded alpha-helical coiled-coil/leucine zipper.

    PubMed Central

    Kohn, W. D.; Kay, C. M.; Hodges, R. S.

    1995-01-01

    The destabilizing effect of electrostatic repulsions on protein stability has been studied by using synthetic two-stranded alpha-helical coiled-coils as a model system. The native coiled-coil consists of two identical 35-residue polypeptide chains with a heptad repeat QgVaGbAcLdQeKf and a Cys residue at position 2 to allow formation of an interchain disulfide bridge. This peptide, designed to contain no intrahelical or interhelical electrostatic interactions, forms a stable coiled-coil structure at 20 degrees C in benign medium (50 mM KCl, 25 mM PO4, pH 7) with a [urea]1/2 value of 6.1 M. Four mutant coiled-coils were designed to contain one or two Glu substitutions for Gln per polypeptide chain. The resulting coiled-coils contained potential i to i' + 5 Glu-Glu interchain repulsions (denoted as peptide E2(15,20)), i to i' + 2 Glu-Glu interchain repulsions (denoted E2(20,22)), or no interchain ionic interactions (denoted E2(13,22) and E1(20)). The stabilities of the coiled-coils were determined by measuring the ellipticities at 222 nm as a function of urea or guanidine hydrochloride concentration at 20 degrees C in the presence and absence of an interchain disulfide bridge. At pH 7, in the presence of urea, the stabilities of E2(13,22) and E2(20,22) were identical suggesting that the potential i to i' + 2 interchain Glu-Glu repulsion in the E2(20,22) coiled-coil does not occur. In contrast, the mutant E2(15,20) is substantially less stable than E2(13,22) or E2(15,20) by 0.9 kcal/mol due to the presence of two i to i' + 5 interchain Glu-Glu repulsions, which destabilize the coiled-coil by 0.45 kcal/mol each. At pH 3 the coiled-coils were found to increase in stability as the number of Glu substitutions were increased. This, combined with reversed-phase HPLC results at pH 7 and pH 2, supports the conclusion that the protonated Glu side chains present at low pH are significantly more hydrophobic than Gln side chains which are in turn more hydrophobic than the ionized

  10. Design and numerical evaluation of a volume coil array for parallel MR imaging at ultrahigh fields

    PubMed Central

    Pang, Yong; Wong, Ernest W.H.; Yu, Baiying

    2014-01-01

    In this work, we propose and investigate a volume coil array design method using different types of birdcage coils for MR imaging. Unlike the conventional radiofrequency (RF) coil arrays of which the array elements are surface coils, the proposed volume coil array consists of a set of independent volume coils including a conventional birdcage coil, a transverse birdcage coil, and a helix birdcage coil. The magnetic fluxes of these three birdcage coils are intrinsically cancelled, yielding a highly decoupled volume coil array. In contrast to conventional non-array type volume coils, the volume coil array would be beneficial in improving MR signal-to-noise ratio (SNR) and also gain the capability of implementing parallel imaging. The volume coil array is evaluated at the ultrahigh field of 7T using FDTD numerical simulations, and the g-factor map at different acceleration rates was also calculated to investigate its parallel imaging performance. PMID:24649435

  11. Performance of an induction coil gun

    SciTech Connect

    Shokair, I.R.; Cowan, M.; Kaye, R.J.; Marder, B.M.

    1993-10-01

    Performance of an electromagnetic induction launcher is considered for three types of armatures. These are: Solid, 1-element wound and 16-element wound aluminum ar natures. The one element wound armature has uniform current density throughout. Because of the radial distribution of the current density, the wound armature can withstand field reversal (working against embedded flux in the armature) and still maintain low temperature. Slingshot simulations were performed, for several configurations. Best performance was obtained for a single element wound armature with two field reversals. For a 60 kg projectile, 10.5 cm coil inner radius and 5.5 cm coil build, the velocity after 50 meters of launcher length (670 stages) exceeded 3.5 km/sec with an overall efficiency of about 45%. For the same parameters the solid and 16-element wound armatures reach a velocity of about 3.3 km/sec after 800 stages (60 meters of launcher length) but without field reversal. A velocity of 3.5 km/sec is possible after 60 meters of launcher length with the 16-element wound armature with one field reversal, but the temperature is close to the melting temperature of aluminum. In all simulations with a solid armature, melting of some of the surface material occurs. However, it is shown that most of the melting occurs after contribution has been made to the forward going pressure, that is, melting does not affect the electrical performance of the launcher. The effect of coil firing tune jitter on launcher performance is also considered and is found to be very small for realistic perturbations. For {plus_minus}2 {mu}-secs random jitter, the reduction in the final velocity for a 60 meter launcher with a solid armature is less than 0.1% and the increase in temperature is only 2%. This result holds for all types of armatures.

  12. Performance of an induction coil launcher

    SciTech Connect

    Shokair, I.R.; Cowan, M.; Kaye, R.J.; Marder, B.M.

    1993-12-31

    Performance of an electromagnetic induction launcher is considered for three types of armatures. These are: Solid, 1-element wound and 16-element wound aluminum armatures. The one element wound armature has uniform current density throughout. Because of the uniformity of the current density, the wound armature can withstand field reversal and still maintain low temperature. Slingshot simulations were performed for several configurations. Best performance was obtained for a single element wound armature with two field reversals. For a 60 kg projectile, 10.5 cm coil inner radius and 5.5 cm coil build, the velocity after 50 meters of launcher length (670 stages) exceeded 3.5 km/sec with an overall efficiency of about 45%. For the same parameters the solid and 16-element wound armatures reach a velocity of about 3.3 km/sec after 800 stages (60 meters of launcher length) but without field reversal. A velocity of 3.5 km/sec is possible after 60 meters of launcher length with the 16-element wound armature with one field reversal, but the temperature is close to the melting temperature of aluminum. In all simulations with a solid armature, melting of some of the surface material occurs. However, it is shown that most of the melting occurs after contribution has been made to the forward going pressure, that is, melting does not affect the electrical performance of the launcher. The effect of coil firing time jitter on launcher performance is also considered and is found to be very small for realistic perturbations. For {plus_minus} 2 {mu}-secs random jitter, the reduction in the final velocity for a 60 meter launcher with a solid armature is less than 0.1% and the increase in temperature is only 2%. This result holds for all types of armatures.

  13. X-ray crystal structure of a TRPM assembly domain reveals an antiparallel four-stranded coiled-coil

    PubMed Central

    Fujiwara, Yuichiro; Minor, Daniel L.

    2008-01-01

    Transient receptor potential (TRP) channels comprise a large family of tetrameric cation-selective ion channels that respond to diverse forms of sensory input. Previous studies have shown that members of the TRPM subclass possess a self-assembling tetrameric C-terminal cytoplasmic coiled-coil domain that underlies channel assembly and trafficking. Here, we present the high-resolution crystal structure of the coiled-coil domain of the channel enzyme TRPM7. The crystal structure, together with biochemical experiments, reveals an unexpected four-stranded antiparallel coiled-coil architecture that bears unique features relative to other antiparallel coiled-coils. Structural analysis indicates that a limited set of interactions encode assembly specificity determinants and uncovers a previously unnoticed segregation of TRPM assembly domains into two families that correspond with the phylogenetic divisions seen for the complete subunits. Together, the data provide a framework for understanding the mechanism of the TRPM channel assembly and highlight the diversity of forms found in the coiled-coil fold. PMID:18782578

  14. X-ray crystal structure of a TRPM assembly domain reveals an antiparallel four-stranded coiled-coil.

    PubMed

    Fujiwara, Yuichiro; Minor, Daniel L

    2008-11-21

    Transient receptor potential (TRP) channels comprise a large family of tetrameric cation-selective ion channels that respond to diverse forms of sensory input. Earlier studies showed that members of the TRPM subclass possess a self-assembling tetrameric C-terminal cytoplasmic coiled-coil domain that underlies channel assembly and trafficking. Here, we present the high-resolution crystal structure of the coiled-coil domain of the channel enzyme TRPM7. The crystal structure, together with biochemical experiments, reveals an unexpected four-stranded antiparallel coiled-coil architecture that bears unique features relative to other antiparallel coiled-coils. Structural analysis indicates that a limited set of interactions encode assembly specificity determinants and uncovers a previously unnoticed segregation of TRPM assembly domains into two families that correspond with the phylogenetic divisions seen for the complete subunits. Together, the data provide a framework for understanding the mechanism of TRPM channel assembly and highlight the diversity of forms found in the coiled-coil fold. PMID:18782578

  15. X-Ray Crystal Structure of a TRPM Assembly Domain Reveals An Antiparallel Four-Stranded Coiled-Coil

    SciTech Connect

    Fujiwara, Y.; Minor, D.L.; Jr.

    2009-05-18

    Transient receptor potential (TRP) channels comprise a large family of tetrameric cation-selective ion channels that respond to diverse forms of sensory input. Earlier studies showed that members of the TRPM subclass possess a self-assembling tetrameric C-terminal cytoplasmic coiled-coil domain that underlies channel assembly and trafficking. Here, we present the high-resolution crystal structure of the coiled-coil domain of the channel enzyme TRPM7. The crystal structure, together with biochemical experiments, reveals an unexpected four-stranded antiparallel coiled-coil architecture that bears unique features relative to other antiparallel coiled-coils. Structural analysis indicates that a limited set of interactions encode assembly specificity determinants and uncovers a previously unnoticed segregation of TRPM assembly domains into two families that correspond with the phylogenetic divisions seen for the complete subunits. Together, the data provide a framework for understanding the mechanism of TRPM channel assembly and highlight the diversity of forms found in the coiled-coil fold.

  16. Comparison of the Association of Sac Growth and Coil Compaction with Recurrence in Coil Embolized Cerebral Aneurysms

    PubMed Central

    2015-01-01

    Background and Purpose In recurrent cerebral aneurysms treated by coil embolization, coil compaction is regarded as the presumptive mechanism. We test the hypothesis that aneurysm growth is the primary recurrence mechanism. We also test the hypothesis that the coil mass will translate a measurable extent when recurrence occurs. Methods An objective, quantitative image analysis protocol was developed to determine the volumes of aneurysms and coil masses during initial and follow-up visits from 3D rotational angiograms. The population consisted of 15 recurrence and 12 non-recurrence control aneurysms initially completely coiled at a single center. An investigator sensitivity study was performed to assess the objectivity of the methods. Paired Wilcoxon tests (p<0.05, one-tailed) were performed to assess for aneurysm and coil growth. The translation of the coil mass center at follow-up was computed. A Mann Whitney U-Test (p<0.05, one-tailed) was used to compare translation of coil mass centers between recurrence and control subjects. Results Image analysis protocol was found to be insensitive to the investigator. Aneurysm growth was evident in the recurrence cohort (p=0.003) but not the control (p=0.136). There was no evidence of coil compaction in either the recurrence or control cohorts (recurrence: p=0.339; control: p=0.429). The translation of the coil mass centers was found to be significantly larger in the recurrence cohort than the control cohort (p=0.047). Conclusion Aneurysm sac growth, not coil compaction, was the primary mechanism of recurrence following successful coil embolization. The coil mass likely translates to a measurable extent when recurrence occurs and has the potential to serve as a non-angiographic recurrence marker. PMID:25894532

  17. Sound Coiled-Tubing Drilling Practices

    SciTech Connect

    Williams, Thomas; Deskins, Greg; Ward, Stephen L.; Hightower, Mel

    2001-09-30

    This Coiled-Tubing Drilling (CTD) Sound Practices Manual provides tools needed by CTD engineers and supervisors to plan, design and perform safe, successful CTD operations. As emphasized throughout, both careful planning and attention to detail are mandatory for success. A bibliography of many useful CTD references is presented in Chapter 6. This manual is organized according to three processes: 1) Pre-Job Planning Process, 2) Operations Execution Process, and 3) Post-Job Review Process. Each is discussed in a logical and sequential format.

  18. Performance verification tests of JT-60SA CS model coil

    NASA Astrophysics Data System (ADS)

    Obana, Tetsuhiro; Murakami, Haruyuki; Takahata, Kazuya; Hamaguchi, Shinji; Chikaraishi, Hirotaka; Mito, Toshiyuki; Imagawa, Shinsaku; Kizu, Kaname; Natsume, Kyohei; Yoshida, Kiyoshi

    2015-11-01

    As a final check of the coil manufacturing method of the JT-60 Super Advanced (JT-60SA) central solenoid (CS), we verified the performance of a CS model coil. The model coil comprised a quad-pancake wound with a Nb3Sn cable-in-conduit conductor. Measurements of the critical current, joint resistance, pressure drop, and magnetic field were conducted in the verification tests. In the critical-current measurement, the critical current of the model coil coincided with the estimation derived from a strain of -0.62% for the Nb3Sn strands. As a result, critical-current degradation caused by the coil manufacturing process was not observed. The results of the performance verification tests indicate that the model coil met the design requirements. Consequently, the manufacturing process of the JT-60SA CS was established.

  19. Toroid cavity/coil NMR multi-detector

    DOEpatents

    Gerald, II, Rex E.; Meadows, Alexander D.; Gregar, Joseph S.; Rathke, Jerome W.

    2007-09-18

    An analytical device for rapid, non-invasive nuclear magnetic resonance (NMR) spectroscopy of multiple samples using a single spectrometer is provided. A modified toroid cavity/coil detector (TCD), and methods for conducting the simultaneous acquisition of NMR data for multiple samples including a protocol for testing NMR multi-detectors are provided. One embodiment includes a plurality of LC resonant circuits including spatially separated toroid coil inductors, each toroid coil inductor enveloping its corresponding sample volume, and tuned to resonate at a predefined frequency using a variable capacitor. The toroid coil is formed into a loop, where both ends of the toroid coil are brought into coincidence. Another embodiment includes multiple micro Helmholtz coils arranged on a circular perimeter concentric with a central conductor of the toroid cavity.

  20. A resolution for the coiling direction paradox in Neogloboquadrina pachyderma

    NASA Astrophysics Data System (ADS)

    Darling, Kate F.; Kucera, Michal; Kroon, Dick; Wade, Christopher M.

    2006-06-01

    We present new data on genotypic differences and biogeographic distribution of coiling types in the living planktonic foraminiferal morphospecies Neogloboquadrina pachyderma. The genetic evidence demonstrates that coiling direction in N. pachyderma is a genetic trait, heritable through time, and is not a morphological feature reflecting ecophenotypic variation. The two opposite coiling morphotypes appear to have diverged during the late Miocene, and they have distinctly different ecologies. In combination with fossil evidence, biogeography, and ecology the degree of genetic distinction between the two coiling types of N. pachyderma strongly implies that they should be considered different species. We propose the adoption of the widely recognized name N. incompta for the right coiling morphospecies. The genetic evidence also demonstrates a low level (<3%) of aberrant coiling associated with both morphotypes. The abundance of these aberrant specimens has no relationship with the environment. These findings have important consequences for the use of N. pachyderma and N. incompta as paleoceanographic signal carriers in polar and subpolar waters.

  1. Ferrite core non-linearity in coils for magnetic neurostimulation

    PubMed Central

    Lazzi, Gianluca

    2014-01-01

    The need to correctly predict the voltage across terminals of mm-sized coils, with ferrite core, to be employed for magnetic stimulation of the peripheral neural system is the motivation for this work. In such applications, which rely on a capacitive discharge on the coil to realise a transient voltage curve of duration and strength suitable for neural stimulation, the correct modelling of the non-linearity of the ferrite core is critical. A demonstration of how a finite-difference model of the considered coils, which include a model of the current-controlled inductance in the coil, can be used to correctly predict the time-domain voltage waveforms across the terminals of a test coil is presented. Five coils of different dimensions, loaded with ferrite cores, have been fabricated and tested: the measured magnitude and width of the induced pulse are within 10% of simulated values. PMID:26609390

  2. Poloidal field coil stress analysis for the ZTH machine

    SciTech Connect

    Girrens, S.P.; Bennett, J.G.; Murphy, D.M.

    1988-02-01

    Three-dimensional finite element analysis of representative equilibrium field and ohmic heating coils for the Los Alamos ZTH air core machine was performed to determine static stress levels developed within the coil structure caused by Lorentz-force loading. Because of the complex coil configuration in cross section (copper conductors embedded in an epoxy insulating matrix), the study was performed in three steps: a bulk orthotropic material property determination, a bulk material coil section analysis, and a detailed composite cross section of selected thickness analysis. Computational procedures used with coil stress and displacement results that were obtained are presented. Extensive work was performed to investigate the magnitude of the error in coil stress predictions arising from the use of the three-step analysis procedure. Analytical and numerical procedures used to perform the estimate of error study are also presented. 34 figs.

  3. The cos-theta coil re-re-visited

    NASA Astrophysics Data System (ADS)

    Crawford, Christopher

    2013-10-01

    Precision measurement of symmetry violating effects such the electric dipole moment (EDM) of fundamental particles requires extremely uniform fields. The cos-theta coil is the standard workhorse for generating uniform transverse magnetic fields in these experiments. Limitations in field uniformity include fringe effects (finite length), discretization (finite number of wires), and construction tolerance (finite resources). The field can be isolated from its environment by superposition of two coaxial cos-theta coils of different radii and opposite magnetic moment (double-cos-theta coil), or by shielding the coil inside a permeable or superconducting cylinder. I will discuss methods for optimizing the field uniformity of a compact cos-theta coil, and compare the ultimate limit on errors due to each source described above. Within the context of the scalar potential, I will show a straightforward generalization to non-circular coils with z- or ϕ-symmetry. Supported in part by DOE contract DE-SC0008107.

  4. Principles Governing the Self-Assembly of Coiled-Coil Protein Nanoparticles.

    PubMed

    Indelicato, Giuliana; Wahome, Newton; Ringler, Philippe; Müller, Shirley A; Nieh, Mu-Ping; Burkhard, Peter; Twarock, Reidun

    2016-02-01

    Self-assembly refers to the spontaneous organization of individual building blocks into higher order structures. It occurs in biological systems such as spherical viruses, which utilize icosahedral symmetry as a guiding principle for the assembly of coat proteins into a capsid shell. In this study, we characterize the self-assembling protein nanoparticle (SAPN) system, which was inspired by such viruses. To facilitate self-assembly, monomeric building blocks have been designed to contain two oligomerization domains. An N-terminal pentameric coiled-coil domain is linked to a C-terminal coiled-coil trimer by two glycine residues. By combining monomers with inherent propensity to form five- and threefold symmetries in higher order agglomerates, the supposition is that nanoparticles will form that exhibit local and global symmetry axes of order 3 and 5. This article explores the principles that govern the assembly of such a system. Specifically, we show that the system predominantly forms according to a spherical core-shell morphology using a combination of scanning transmission electron microscopy and small angle neutron scattering. We introduce a mathematical toolkit to provide a specific description of the possible SAPN morphologies, and we apply it to characterize all particles with maximal symmetry. In particular, we present schematics that define the relative positions of all individual chains in the symmetric SAPN particles, and provide a guide of how this approach can be generalized to nonspherical morphologies, hence providing unprecedented insights into their geometries that can be exploited in future applications. PMID:26840729

  5. An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors

    PubMed Central

    Shekhawat, Sujan S.; Porter, Jason R.; Sriprasad, Akshay; Ghosh, Indraneel

    2009-01-01

    Proteases are widely studied as they are integral players in cell cycle control and apoptosis. We report a new approach for the design of a family of genetically encoded turn-on protease biosensors. In our design, an auto-inhibited coiled-coil switch is turned on upon proteolytic cleavage, which results in the complementation of split-protein reporters. Utilizing this new auto-inhibition design paradigm, we present the rational construction and optimization of three generations of protease biosensors, with the final design providing a 1000 fold increase in bioluminescent signal upon addition of the TEV protease. We demonstrate the generality of the approach utilizing two different split-protein reporters, firefly luciferase and beta-lactamase, while also testing our design in the context of a therapeutically relevant protease, caspase-3. Finally, we present a dual-protease sensor geometry that allows for the use of these turn-on sensors as potential AND logic gates. Thus these studies potentially provide a new method for the design and implementation of genetically encoded turn-on protease sensors while also providing a general auto-inhibited coiled-coil strategy for controlling the activity of fragmented proteins. PMID:19803505

  6. Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly

    NASA Astrophysics Data System (ADS)

    White, Simon J.; Johnson, Steven; Szymonik, Michal; Wardingley, Richard A.; Pye, Douglas; Davies, A. Giles; Wälti, Christoph; Stockley, Peter G.

    2012-12-01

    Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode.

  7. Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly.

    PubMed

    White, Simon J; Johnson, Steven; Szymonik, Michal; Wardingley, Richard A; Pye, Douglas; Davies, A Giles; Wälti, Christoph; Stockley, Peter G

    2012-12-14

    Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode. PMID:23154792

  8. Transmembrane and coiled-coil domain family 1 is a novel protein of the endoplasmic reticulum.

    PubMed

    Zhang, Chao; Kho, Yik-Shing; Wang, Zhe; Chiang, Yan Ting; Ng, Gary K H; Shaw, Pang-Chui; Wang, Yuzhuo; Qi, Robert Z

    2014-01-01

    The endoplasmic reticulum (ER) is a continuous membrane network in eukaryotic cells comprising the nuclear envelope, the rough ER, and the smooth ER. The ER has multiple critical functions and a characteristic structure. In this study, we identified a new protein of the ER, TMCC1 (transmembrane and coiled-coil domain family 1). The TMCC family consists of at least 3 putative proteins (TMCC1-3) that are conserved from nematode to human. We show that TMCC1 is an ER protein that is expressed in diverse human cell lines. TMCC1 contains 2 adjacent transmembrane domains near the C-terminus, in addition to coiled-coil domains. TMCC1 was targeted to the rough ER through the transmembrane domains, whereas the N-terminal region and C-terminal tail of TMCC1 were found to reside in the cytoplasm. Moreover, the cytosolic region of TMCC1 formed homo- or hetero-dimers or oligomers with other TMCC proteins and interacted with ribosomal proteins. Notably, overexpression of TMCC1 or its transmembrane domains caused defects in ER morphology. Our results suggest roles of TMCC1 in ER organization. PMID:24454821

  9. Peptidyl Materials Formed Through Click Chemistry Enhanced Coiled-Coil Interactions

    NASA Astrophysics Data System (ADS)

    Koehler, Kenneth

    2014-03-01

    Biologically derived materials offer a level of sophistication synthetically fabricated materials have only attempted to mimic. This level of complexity may be found in materials such as peptides. Implementing new theory and modeling, peptides with the propensity to form coiled-coil (CC) bundles were designed and synthesized. Through the use of this de novo approach, modeling allowed prediction of the feasibility to include non-natural amino acids conducive to click chemistry into the peptide. Amino acids showcasing thiol or alkyne functionalities were considered owing to the ability of these moieties to participate in the thiol-ene and copper click reactions respectively. Once synthesized, the peptides decorated with these clickable motifs were placed in solution and allowed to self-assemble into CC's. CD spectroscopy and DLS experiments confirmed the formation and assembly of CC's. Click reactions were then incited to link the CC assemblies together and form a network with predictable dimensionality and pore size between CC bundles. To incite network formation, click reactions between CC side chain residues and suitably functionalized crosslinkers were implemented. The linking of coiled-coils and material formation were assessed using DLS and TEM.

  10. The Orientations of Large Aspect-Ratio Coiled-Coil Proteins Attached to Gold Nanostructures.

    PubMed

    Chang, Jae-Byum; Kim, Yong Ho; Thompson, Evan; No, Young Hyun; Kim, Nam Hyeong; Arrieta, Jose; Manfrinato, Vitor R; Keating, Amy E; Berggren, Karl K

    2016-03-01

    Methods for patterning biomolecules on a substrate at the single molecule level have been studied as a route to sensors with single-molecular sensitivity or as a way to probe biological phenomena at the single-molecule level. However, the arrangement and orientation of single biomolecules on substrates has been less investigated. Here, the arrangement and orientation of two rod-like coiled-coil proteins, cortexillin and tropomyosin, around patterned gold nanostructures is examined. The high aspect ratio of the coiled coils makes it possible to study their orientations and to pursue a strategy of protein orientation via two-point attachment. The proteins are anchored to the surfaces using thiol groups, and the number of cysteine residues in tropomyosin is varied to test how this variation affects the structure and arrangement of the surface-attached proteins. Molecular dynamics studies are used to interpret the observed positional distributions. Based on initial studies of protein attachment to gold post structures, two 31-nm-long tropomyosin molecules are aligned between the two sidewalls of a trench with a width of 68 nm. Because the approach presented in this study uses one of twenty natural amino acids, this method provides a convenient way to pattern biomolecules on substrates using standard chemistry. PMID:26799936

  11. Selective amine labeling of cell surface proteins guided by coiled-coil assembly.

    PubMed

    Yano, Yoshiaki; Furukawa, Nami; Ono, Satoshi; Takeda, Yuki; Matsuzaki, Katsumi

    2016-11-01

    Covalent labeling of target proteins in living cells is useful for both fluorescence live-cell imaging and the subsequent biochemical analyses of the proteins. Here, we report an efficient method for the amine labeling of membrane proteins on the cell surface, guided by a noncovalent coiled-coil interaction. A carboxyl sulfosuccinimidyl ester introduced at the C-terminus of the coiled-coil probe reacted with target proteins under mild labeling conditions ([probe] = 150 nM, pH 7.4, 25°C) for 20 min. Various fluorescent moieties with different hydrophobicities are available for covalent labeling with high signal/background labeling ratios. Using this method, oligomeric states of glycophorin A (GpA) were compared in mammalian CHO-K1 cells and sodium dodecyl sulfate (SDS) micelles. In the cell membranes, no significant self-association of GpA was detected, whereas SDS-PAGE suggested partial dimerization of the proteins. Membrane cholesterol was found to be an important factor that suppressed the dimerization of GpA. Thus, the covalent functionality enables direct comparison of the oligomeric state of membrane proteins under various conditions. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 484-490, 2016. PMID:26285787

  12. Structure of a Designed, Right-Handed Coiled-Coil Tetramer Containing All Biological Amino Acids

    SciTech Connect

    Sales, M.; Plecs, J.J.; Holton, J.M.; Alber, T.

    2009-06-04

    The previous design of an unprecedented family of two-, three-, and four-helical, right-handed coiled coils utilized nonbiological amino acids to efficiently pack spaces in the oligomer cores. Here we show that a stable, right-handed parallel tetrameric coiled coil, called RH4B, can be designed entirely using biological amino acids. The X-ray crystal structure of RH4B was determined to 1.1 {angstrom} resolution using a designed metal binding site to coordinate a single Yb{sup 2+} ion per 33-amino acid polypeptide chain. The resulting experimental phases were particularly accurate, and the experimental electron density map provided an especially clear, unbiased view of the molecule. The RH4B structure closely matched the design, with equivalent core rotamers and an overall root-mean-square deviation for the N-terminal repeat of the tetramer of 0.24 {angstrom}. The clarity and resolution of the electron density map, however, revealed alternate rotamers and structural differences between the three sequence repeats in the molecule. These results suggest that the RH4B structure populates an unanticipated variety of structures.

  13. Improvement of probe peptides for coiled-coil labeling by introducing phosphoserines.

    PubMed

    Ono, Satoshi; Yano, Yoshiaki; Matsuzaki, Katsumi

    2012-01-01

    We have developed a method of rapidly labeling membrane proteins in living cells using a high-affinity heterodimeric coiled-coil construct containing an E3 tag (EIAALEK)(3) genetically fused to the target protein and a K4 probe (KIAALKE)(4) labeled with a fluorophore such as tetramethylrhodamine (TMR) at its N-terminus (TMR-K4). However, coiled-coil labeling cannot be applied to highly negatively charged cell lines such as HEK293, because of the nonspecific adsorption of the positively charged K4 probes to cell membranes. To reduce the net positive charge, we synthesized new probes that include phosphoserine residues (pSer) between the K4 sequence and TMR fluorophore (TMR-(pSer)(n)-K4, [n = 1-3]). The affinity of the pSer-introduced probes was comparable to that of the TMR-K4 probe. However, the TMR-(pSer)(2)-K4 and TMR-(pSer)(3)-K4 probes tended to aggregate during labeling. In contrast, TMR-pSer-K4, which was as soluble as TMR-K4, achieved higher signal/background ratios (30-100) for four host cell lines (HEK293, HeLa, SH-SY5Y, and PC12) than did TMR-K4 (~10 for HEK293 cells), demonstrating that the improved probe can be used for various types of cells. PMID:22782565

  14. Immunogenicity of coiled-coil based drug-free macromolecular therapeutics

    PubMed Central

    Kverka, Miloslav; Hartley, Jonathan M.; Chu, Te-Wei; Yang, Jiyuan; Heidchen, Regina; Kopeček, Jindřich

    2014-01-01

    A two-component CD20 (non-internalizing) receptor crosslinking system based on the biorecognition of complementary coiled-coil forming peptides was evaluated. Exposure of B cells to Fab’-peptide1 conjugate decorates the cell surface with peptide1; further exposure of the decorated cells to P-(peptide2)x (P is the N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer backbone) results in the formation of coiled-coil heterodimers at the cell surface with concomitant induction of apoptosis. The aim of this study was to determine the potential immunogenicity of this therapeutic system that does not contain low molecular weight drugs. Enantiomeric peptides (L- and D-CCE and L- and D-CCK), HPMA copolymer-peptide conjugates, and Fab’ fragment-peptide conjugates were synthesized and the immunological properties of peptide conjugates evaluated in vitro on RAW264.7 macrophages and in vivo on immunocompetent BALB/c mice. HPMA copolymer did not induce immune response in vitro and in vivo. Administration of P-peptide conjugates with strong adjuvant resulted in antibody response directed to the peptide. Fab’ was responsible for macrophage activation of Fab’-peptide conjugates and a major factor in the antibody induction following i.v. administration of Fab’ conjugates. There was no substantial difference in the ability of conjugates of D-peptides and conjugates of L-peptides to induce Ab response. PMID:24767787

  15. Laminar flow effects in the coil planet centrifuge

    NASA Technical Reports Server (NTRS)

    Herrmann, F. T.

    1984-01-01

    The coil planet centrifuge designed by Ito employs flow of a single liquid phase, through a rotating coiled tube in a centrifugal force field, to provide a separation of particles based on sedimentation rates. Mathematical solutions are derived for the linear differential equations governing particle behavior in the coil planet centrifuge device. These solutions are then applied as the basis of a model for optimizing particle separations.

  16. Magnet coils made from high-temperature superconductor

    NASA Astrophysics Data System (ADS)

    Jenkins, R. G.; Jones, H.; Burgoyne, J.; Yang, M.; Grovenor, C. R. M.; Goringe, M. J.

    1996-02-01

    We review the progress we have made in constructing HTS coils and report our latest results. Also we describe the cryogen-free operation of one of our HTS coils cooled to 55 K using a Stirling cycle cryocooler. Lastly, we describe how 4 Oxford coils are being used in a project to investigate the controllability of HTS magnets in applications such as “maglev” suspension systems. We briefly report the initial findings of this work and describe developments in progress.

  17. Method for manufacturing a rotor having superconducting coils

    DOEpatents

    Driscoll, David I.; Shoykhet, Boris A.

    2001-01-01

    A method and apparatus for manufacturing a rotor for use with a rotating machine is provided that employs a superconducting coil on the rotor. An adhesive is applied to an outer surface of the rotor body, which may include a groove disposed within an outer surface of the rotor body. A superconducting coil is then mounted onto the rotor body such that the adhesive bonds the superconducting coil to the rotor body.

  18. RF Field Distributions in a Slotted-Type Lisitano Coil

    NASA Astrophysics Data System (ADS)

    Suetsugu, Yusuke; Kawai, Yoshinobu

    1984-08-01

    The field distributions in a slotted-type Lisitano coil are analyzed using a simple antenna model, and the microwave power is theoretically found to be supplied near the center of the coil. Field intensities in the Lisitano coil measured with a calibrated loop antenna are compared with the theoretical values, and results supporting the analysis are obtained. The field distributions in a vacuum chamber were also measured.

  19. Stabilization of Tokamak Plasmas by the Addition of Nonaxisymmetric Coils

    NASA Astrophysics Data System (ADS)

    Reiman, Allan

    2008-11-01

    It has been recognized since the early days of the fusion program that stellarator coils can be used to stabilize current carrying, toroidal, magnetically confined plasmas.[1] More recently, it has been shown that the vertical mode in a tokamak can be stabilized by a relatively simple set of parallelogram-shaped, localized, nonaxisymmetric coils.[2] We show that by superposing sets of these parallelogram-shaped, nonaxisymmetric coils at different locations, it is possible to reproduce the coil current patterns for conventional stellarator coils as well as those for Furth-Hartman coils[3]. This allows us to gain insight into the physics of stabilization produced by various sets of nonaxisymmetric coils by analysis of the effect on stability of localized coils at differing locations. In particular, the relationship between the stabilization effect and the rotational transform generated by the nonaxisymmetric coils is clarified. [1] J. L. Johnson, C. R. Oberman, R. M. Kulsrud, and E. A. Frieman, Phys. Fluids 1, 281 (1958) [2] A. Reiman, Phys. Rev. Lett. 99, 135007, (2007). [3] H.P. Furth and C.W. Hartman, Phys. Fluids 11, 408 (1968).

  20. Coiled tubing used for slim hole re-entry

    SciTech Connect

    Traonmilin, E. ); Newman, K. )

    1992-02-17

    A coiled tubing unit with slim hole tools successfully re-entered and cored an existing Elf Aquitaine vertical well in the Paris basin in France. This experiment proved that coiled tubing could be used to drill, core, and test a slim hole well. Elf Aquitaine studied the use of coiled tubing for drilling inexpensive exploration wells in the Paris basin. As a result of this study, Elf believed that coiled tubing exploration drilling could significantly reduce exploration costs. This paper reports on a number of questions raised by this study: Can coiled tubing be used effectively to drill slim open hole How would the drilling rate compare with that of a conventional drilling rig If the rate were too slow, coiled tubing might not be economical. Can a straight vertical well be drilled Coiled tubing pipe has a residual curvature from bending over the reel and gooseneck. Will this curvature make it impossible to drill straight Can the coiled tubing also be used to take cores Once the hole is drilled, can it be tested with coiled tubing

  1. A novel acoustically quiet coil for neonatal MRI system

    PubMed Central

    Ireland, Christopher M.; Giaquinto, Randy O.; Loew, Wolfgang; Tkach, Jean A.; Pratt, Ronald G.; Kline-Fath, Beth M.; Merhar, Stephanie L.; Dumoulin, Charles L.

    2015-01-01

    MRI acoustic exposure has the potential to elicit physiological distress and impact development in preterm and term infants. To mitigate this risk, a novel acoustically quiet coil was developed to reduce the sound pressure level experienced by neonates during MR procedures. The new coil has a conventional high-pass birdcage RF design, but is built on a framework of sound abating material. We evaluated the acoustic and MR imaging performance of the quiet coil and a conventional body coil on two small footprint NICU MRI systems. Sound pressure level and frequency response measurements were made for six standard clinical MR imaging protocols. The average sound pressure level, reported for all six imaging pulse sequences, was 82.2 dBA for the acoustically quiet coil, and 91.1 dBA for the conventional body coil. The sound pressure level values measured for the acoustically quiet coil were consistently lower, 9 dBA (range 6-10 dBA) quieter on average. The acoustic frequency response of the two coils showed a similar harmonic profile for all imaging sequences. However, the amplitude was lower for the quiet coil, by as much as 20 dBA. PMID:26457072

  2. Switching transients in the MFTF yin-yang coils

    SciTech Connect

    Owen, E.W.; Shimer, D.W.

    1982-11-02

    This report is a study of the transients caused by the fast dump of large superconducting coils. Theoretical analysis, computer simulation, and actual measurements are used. Theoretical analysis can only be applied to the simplest of models. In the computer simulations two models are used, one in which the coil is divided into ten segments and another in which a single coil is employed. The circuit breaker that interrupts the current to the power supply, causing a fast dump, is represented by a time and current dependent conductance. Actual measurements are limited to measurements made incidental to the coils' performance tests.

  3. Service life of counter-current chromatography coils.

    PubMed

    Conway, Walter D

    2007-06-01

    A multilayer coil of PTFE tubing, which failed after being used each workday for about 3 years in a type J centrifuge, was examined. Two types of defects were found. One, called crazes, occurs throughout the coil and does not leak initially, but may eventually lead to a short, axially oriented slit. Another, called indentations, is seen primarily in the innermost and other nearby layers. They are elongated, about 5 mm, indentations, usually on the central side of the tubing. These eventually crack and leak. PTFE tubing is permeable to air and hexane and expands by more than 1% when immersed in hexane, heptane or chloroform for a few days. It is suggested that the crazes result from exposure of the somewhat flexible tubing to the undulating centripetal force field in the coil-planet centrifuge, especially when further softened by solvent absorption. The indentations may result from carriage of the excess tubing length, created by solvent absorption, from the coil periphery to the coil center by the centripetal force field, which continuously travels from the peripheral tail to the central head of the coil. A 1% increase in coil length creates 74 cm of excess tubing in the 160-ml coils examined in this study. It is suggested that fluorinated ethylene propylene (FEP) tubing, especially when etched on the outside, may provide more stable CCC coils, since its expansion when exposed to organic solvents is 0.1 or less than that of PTFE. PMID:17428490

  4. Coil Array Design Inspired on the Kepler's Lenten Pretzel

    SciTech Connect

    Vazquez, F.; Solis, S. E.; Rodriguez, A. O.

    2008-08-11

    The RF coil arrays are an important part in Magnetic Resonance Imaging, since they are the main device for transmission and reception of the magnetic resonance signal. An RF coil array with a new configuration based on the Kepler's Lenten pretzel for the geocentric path of Mars is proposed in this work. The evenly distributed trajectories may serve as the basic configuration to form a coil array to adequately cover a region of interest for magnetic resonance experiments. The main goal is to investigate the electromagnetic properties of this coil array geometry to obtain an optimal design for its further construction. Hence, the electromagnetic properties of the coil array were numerical simulated using the finite element method and the quasi-static approach. Resulting simulations showed that there is an important concentration of magnetic field lines at the centre of the coil array. This is an advantage over other coil arrays where the magnetic field usually decreased at their geometrical centre. Both the electric and magnetic fields had also a very good uniformity. These characteristics made this coil design a good candidate for applications where the use of multi-coil technology is mandatory.

  5. Dirty air conditioners: Energy implications of coil fouling

    SciTech Connect

    Siegel, Jeffrey; Walker, Iain; Sherman, Max

    2002-03-01

    Residential air conditioning is responsible for a substantial amount of peak electrical demand and energy consumption throughout most of the United States. Coil fouling, the deposition of indoor dusts and other particulate matter on evaporator heat exchangers, increases system pressure drop and, correspondingly, decreases system air flow and air conditioner performance. In this paper, we apply experimental and simulation results describing particle deposition on evaporator coils as well as research about indoor particle and dust concentrations to determine coil fouling rates. The results suggest that typical coils foul enough to double evaporator pressure drop in about 7.5 years, much sooner than the expected 15-30 year life time for an evaporator coil. The most important parameters in determining coil fouling times are the efficiency of the filter and indoor particle concentrations, although filter bypass and duct and coil design are important as well. The reduced air flows that result from coil fouling cause typical efficiency and capacity degradations of less than 5%, however they can be much greater for marginal systems or extreme conditions. These energy issues, as well as possible indoor air quality issues resulting from fouling by biological aerosols, suggest that regular coil cleaning to ameliorate low flow and the elimination of filter bypass should be an important part of residential air conditioning commissioning and maintenance practices.

  6. The asymptotic structure of a slender coiling fluid thread

    NASA Astrophysics Data System (ADS)

    Blount, Maurice; Lister, John

    2010-11-01

    The buckling of a viscous fluid thread as it falls through air onto a stationary surface is a well-known breakfast-time phenomenon which exhibits a rich variety of dynamical regimes [1]. Since the bending resistance of a slender thread is small, bending motion is largely confined to a short region of coiling near the surface. If the height of fall is large enough, then the thread above the coiling region forms a `tail' that falls nearly vertically under gravity but is deflected slightly due to forces exerted on it by the coil. Although it is possible to use force balances in the coil to estimate scalings for the coiling frequency, we analyse the solution structure of the entire thread in the asymptotic limit of a very slender thread and thereby include the dynamic interaction between the coil and the tail. Quantitative predictions of the coiling frequency are obtained which demonstrate the existence of leading-order corrections to scalings previously derived. In particular, we show that in the regime where the deflection of the tail is governed by a balance between centrifugal acceleration, hoop stress and gravity, the tail behaves as a flexible circular pendulum that is forced by bending stress exerted by the coil. The amplitude of the response is calculated and the previously observed resonance when the coiling frequency coincides with one of the eigenfrequencies of a free flexible pendulum is thereby explained. [1] N.M. Ribe et al., J. Fluid Mech. 555, 275-297.

  7. Dental MRI using wireless intraoral coils

    PubMed Central

    Ludwig, Ute; Eisenbeiss, Anne-Katrin; Scheifele, Christian; Nelson, Katja; Bock, Michael; Hennig, Jürgen; von Elverfeldt, Dominik; Herdt, Olga; Flügge, Tabea; Hövener, Jan-Bernd

    2016-01-01

    Currently, the gold standard for dental imaging is projection radiography or cone-beam computed tomography (CBCT). These methods are fast and cost-efficient, but exhibit poor soft tissue contrast and expose the patient to ionizing radiation (X-rays). The need for an alternative imaging modality e.g. for soft tissue management has stimulated a rising interest in dental magnetic resonance imaging (MRI) which provides superior soft tissue contrast. Compared to X-ray imaging, however, so far the spatial resolution of MRI is lower and the scan time is longer. In this contribution, we describe wireless, inductively-coupled intraoral coils whose local sensitivity enables high resolution MRI of dental soft tissue. In comparison to CBCT, a similar image quality with complementary contrast was obtained ex vivo. In-vivo, a voxel size of the order of 250∙250∙500 μm3 was achieved in 4 min only. Compared to dental MRI acquired with clinical equipment, the quality of the images was superior in the sensitive volume of the coils and is expected to improve the planning of interventions and monitoring thereafter. This method may enable a more accurate dental diagnosis and avoid unnecessary interventions, improving patient welfare and bringing MRI a step closer to becoming a radiation-free alternative for dental imaging. PMID:27021387

  8. Multi-coil magnetic field modeling

    NASA Astrophysics Data System (ADS)

    Juchem, Christoph; Green, Dan; de Graaf, Robin A.

    2013-11-01

    The performance of multi-coil (MC) magnetic field modeling is compared to dedicated wire patterns for the generation of spherical harmonic (SH) shapes as these are the workhorse for spatial encoding and magnetic field homogenization in MR imaging and spectroscopy. To this end, an example 48 channel MC setup is analyzed and shown to be capable of generating all first through fourth order SH shapes over small and large regions-of-interest relevant for MR investigations. The MC efficiency for the generation of linear gradient fields shares the same order of magnitude with classic and state-of-the-art SH gradient coils. MC field modeling becomes progressively more efficient with the synthesis of more complex field shapes that require the combination of multiple SH terms. The possibility of a region-specific optimization of both magnetic field shapes and generation performance with the MC approach are discussed with emphasis on the possible trade-off between the field accuracy and generation efficiency.

  9. Continuum source tungsten coil atomic fluorescence spectrometry.

    PubMed

    Gu, Jiyan; Donati, George L; Young, Carl G; Jones, Bradley T

    2011-04-01

    A simple continuum source tungsten coil atomic fluorescence spectrometer is constructed and evaluated. The heart of the system is the atomizer: a low-cost tungsten filament extracted from a 150 W light bulb. The filament is resistively heated with a small, solid-state, constant-current power supply. The atomizer is housed in a glass chamber and purged with a 1 L/min flow of a conventional welding gas mixture: 10% H(2)/Ar. A 25 μL sample aliquot is pipetted onto the tungsten coil, the liquid is dried at low current, and then the atomic vapor is produced by applying a current in the range 3.5-5.5 A. The atomization current does not produce temperatures high enough to excite atomic emission. Radiation from a 300 W xenon lamp is focused through the atomic vapor, exciting atomic fluorescence. Fluorescence signals are collected using a hand-held charge-coupled device (CCD) spectrometer. Simultaneous determination of ten elements (Ag, Bi, Cr, Cu, Ga, In, Mg, Mn, and Tl) results in detection limits in the range 0.3 to 10 ng. The application of higher atomization currents (10 A) leads to straightforward detection of atomic emission signals with no modifications to the instrument. PMID:21396184

  10. Dental MRI using wireless intraoral coils.

    PubMed

    Ludwig, Ute; Eisenbeiss, Anne-Katrin; Scheifele, Christian; Nelson, Katja; Bock, Michael; Hennig, Jürgen; von Elverfeldt, Dominik; Herdt, Olga; Flügge, Tabea; Hövener, Jan-Bernd

    2016-01-01

    Currently, the gold standard for dental imaging is projection radiography or cone-beam computed tomography (CBCT). These methods are fast and cost-efficient, but exhibit poor soft tissue contrast and expose the patient to ionizing radiation (X-rays). The need for an alternative imaging modality e.g. for soft tissue management has stimulated a rising interest in dental magnetic resonance imaging (MRI) which provides superior soft tissue contrast. Compared to X-ray imaging, however, so far the spatial resolution of MRI is lower and the scan time is longer. In this contribution, we describe wireless, inductively-coupled intraoral coils whose local sensitivity enables high resolution MRI of dental soft tissue. In comparison to CBCT, a similar image quality with complementary contrast was obtained ex vivo. In-vivo, a voxel size of the order of 250∙250∙500 μm(3) was achieved in 4 min only. Compared to dental MRI acquired with clinical equipment, the quality of the images was superior in the sensitive volume of the coils and is expected to improve the planning of interventions and monitoring thereafter. This method may enable a more accurate dental diagnosis and avoid unnecessary interventions, improving patient welfare and bringing MRI a step closer to becoming a radiation-free alternative for dental imaging. PMID:27021387

  11. MINOS near-detector coil design

    SciTech Connect

    Nelson, J.K.; Kilmer, J.; /Fermilab

    1999-08-01

    The 980-ton MINOS near detector will be installed in the new NuMI near hall at 100m below grade at Fermilab. It will be assembled from 282 1-inch thick steel plates. The planes are made from low carbon (1006), hot rolled steel and are toroidally magnetized. Each plane will be hung by two 'ears', which are extensions of the octagonal plane structure, similar to the hanging files in a file drawer. The plates have a center-to-center spacing of 5.94 cm. This document has been prepared for the 8/99 Conceptual Design Review of the MINOS near detector coil. It's main goal is to provide a set of references to previous documents and to assemble various design drawings and engineering calculations that have not been included in previous technical memos. It also provides some background material relevant for the coil implementation. Much of the text for this document is edited from the MINOS Detectors Technical Design Report.

  12. Dental MRI using wireless intraoral coils

    NASA Astrophysics Data System (ADS)

    Ludwig, Ute; Eisenbeiss, Anne-Katrin; Scheifele, Christian; Nelson, Katja; Bock, Michael; Hennig, Jürgen; von Elverfeldt, Dominik; Herdt, Olga; Flügge, Tabea; Hövener, Jan-Bernd

    2016-03-01

    Currently, the gold standard for dental imaging is projection radiography or cone-beam computed tomography (CBCT). These methods are fast and cost-efficient, but exhibit poor soft tissue contrast and expose the patient to ionizing radiation (X-rays). The need for an alternative imaging modality e.g. for soft tissue management has stimulated a rising interest in dental magnetic resonance imaging (MRI) which provides superior soft tissue contrast. Compared to X-ray imaging, however, so far the spatial resolution of MRI is lower and the scan time is longer. In this contribution, we describe wireless, inductively-coupled intraoral coils whose local sensitivity enables high resolution MRI of dental soft tissue. In comparison to CBCT, a similar image quality with complementary contrast was obtained ex vivo. In-vivo, a voxel size of the order of 250•250•500 μm3 was achieved in 4 min only. Compared to dental MRI acquired with clinical equipment, the quality of the images was superior in the sensitive volume of the coils and is expected to improve the planning of interventions and monitoring thereafter. This method may enable a more accurate dental diagnosis and avoid unnecessary interventions, improving patient welfare and bringing MRI a step closer to becoming a radiation-free alternative for dental imaging.

  13. A Compact Non-Planar Coil Design for the SFLM Hybrid

    NASA Astrophysics Data System (ADS)

    Hagnestål, A.; Ågren, O.; Moiseenko, V. E.

    2012-08-01

    A non-planar single layer semiconductor coil set for a version of the Straight Field Line Mirror Hybrid concept with reduced magnetic field has been computed. The coil set consists of 30 coils that are somewhat similar to baseball coils with skewed sides. The coil set has been modeled with filamentary current distributions and basic scaling assumptions have been made regarding the coil widths. This coil set is expected to be considerably cheaper than a previous computed coil set. The coils can probably be produced with technologies known today.

  14. Effect of Inductive Coil Shape on Sensing Performance of Linear Displacement Sensor Using Thin Inductive Coil and Pattern Guide

    PubMed Central

    Misron, Norhisam; Ying, Loo Qian; Firdaus, Raja Nor; Abdullah, Norrimah; Mailah, Nashiren Farzilah; Wakiwaka, Hiroyuki

    2011-01-01

    This paper discusses the effect of inductive coil shape on the sensing performance of a linear displacement sensor. The linear displacement sensor consists of a thin type inductive coil with a thin pattern guide, thus being suitable for tiny space applications. The position can be detected by measuring the inductance of the inductive coil. At each position due to the change in inductive coil area facing the pattern guide the value of inductance is different. Therefore, the objective of this research is to study various inductive coil pattern shapes and to propose the pattern that can achieve good sensing performance. Various shapes of meander, triangular type meander, square and circle shape with different turn number of inductive coils are examined in this study. The inductance is measured with the sensor sensitivity and linearity as a performance evaluation parameter of the sensor. In conclusion, each inductive coil shape has its own advantages and disadvantages. For instance, the circle shape inductive coil produces high sensitivity with a low linearity response. Meanwhile, the square shape inductive coil has a medium sensitivity with higher linearity. PMID:22346656

  15. Meta-analysis of stent-assisted coiling versus coiling-only for the treatment of intracranial aneurysms.

    PubMed

    Phan, Kevin; Huo, Ya R; Jia, Fangzhi; Phan, Steven; Rao, Prashanth J; Mobbs, Ralph J; Mortimer, Alex M

    2016-09-01

    Endovascular coil embolization is a widely accepted and useful treatment modality for intracranial aneurysms. However, the principal limitation of this technique is the high aneurysm recurrence. The adjunct use of stents for coil embolization procedures has revolutionized the field of endovascular aneurysm management, however its safety and efficacy remains unclear. Two independent reviewers searched six databases from inception to July 2015 for trials that reported outcomes according to those who received stent-assisted coiling versus coiling-only (no stent-assistance). There were 14 observational studies involving 2698 stent-assisted coiling and 29,388 coiling-only patients. The pooled immediate occlusion rate for stent-assisted coiling was 57.7% (range: 20.2%-89.2%) and 48.7% (range: 31.7%-89.2%) for coiling-only, with no significant difference between the two (odds ratio [OR}=1.01; 95% confidence intervals [CI}: 0.68-1.49). However, progressive thrombosis was significantly more likely in stent-assisted coiling (29.9%) compared to coiling-only (17.5%) (OR=2.71; 95% CI: 1.95-3.75). Aneurysm recurrence was significantly lower in stent-assisted coiling (12.7%) compared to coiling-only (27.9%) (OR=0.43; 95% CI: 0.28-0.66). In terms of complications, there was no significant difference between the two techniques for all-complications, permanent complications or thrombotic complications. Mortality was significantly higher in the stent-assisted group 1.4% (range: 0%-27.5%) compared to the coiling-only group 0.2% (range: 0%-19.7%) (OR=2.16; 95% CI: 1.33-3.52). Based on limited evidence, stent-assisted coiling shows similar immediate occlusion rates, improved progressive thrombosis and decreased aneurysm recurrence compared to coiling-only, but is associated with a higher mortality rate. Future randomized controlled trials are warranted to clarify the safety of stent-associated coiling. PMID:27344091

  16. Development of Ground Coils with Low Eddy Current Loss by Applying the Compression Molding Method after the Coil Winding

    NASA Astrophysics Data System (ADS)

    Suzuki, Masao; Aiba, Masayuki; Takahashi, Noriyuki; Ota, Satoru; Okada, Shigenori

    In a magnetically levitated transportation (MAGLEV) system, a huge number of ground coils will be required because they must be laid for the whole line. Therefore, stable performance and reduced cost are essential requirements for the ground coil development. On the other hand, because the magnetic field changes when the superconducting magnet passes by, an eddy current will be generated in the conductor of the ground coil and will result in energy loss. The loss not only increases the magnetic resistance for the train running but also brings an increase in the ground coil temperature. Therefore, the reduction of the eddy current loss is extremely important. This study examined ground coils in which both the eddy current loss and temperature increase were small. Furthermore, quantitative comparison for the eddy current loss of various magnet wire samples was performed by bench test. On the basis of the comparison, a round twisted wire having low eddy current loss was selected as an effective ground coil material. In addition, the ground coils were manufactured on trial. A favorable outlook to improve the size accuracy of the winding coil and uneven thickness of molded resin was obtained without reducing the insulation strength between the coil layers by applying a compression molding after winding.

  17. Impulse and Frequency Response of a Moving Coil Galvanometer.

    ERIC Educational Resources Information Center

    McNeill, D. J.

    1985-01-01

    Describes an undergraduate laboratory experiment in which a moving coil galvanometer is studied and the electromotive force generated by the swinging coil provides the impulse response information in a form suitable for digitizing and inputing to a microcomputer. Background information and analysis of typical data are included. (JN)

  18. New Development of VPI Process for Large Superconducting Coils

    NASA Astrophysics Data System (ADS)

    Pan, Wanjiang; Wu, Songtao; Cui, Yimin

    2003-08-01

    High vacuum is required for Vacuum Pressure Impregnation (VPI) process of large coils used in cryogenic. The defects such as dry spots and over rich resins should be minimized in large superconducting coils used. Both sealing problems associated with the mold and over rich resin problems are eliminated by using vacuum bag mold method with which we can simplify the design of vacuum mold.

  19. TECHNOLOGY DEVELOPMENT FOR REACT AND WIND COMMON COIL MAGNETS.

    SciTech Connect

    ESCALLIER,J.; ANERELLA,M.; COZZOLINO,J.; GANETIS,G.; GHOSH,A.; GUPTA,R.; HARRISON,M.; MARONE,A.; MURATORE,J.; PARKER,B.; SAMPSON,W.; WANDERER,P.

    2001-06-18

    High field common coil magnets [1,2] using brittle High Temperature Superconductors (HTS) or Nb{sub 3}Sn cables provide new challenges with respect to the design and manufacturing of coils. We are developing the scaleable techniques that can be used in the production of common coil or other magnets with similar designs [3,4]. By utilizing a cost-effective rapid turnaround short coil program, it is possible to quickly develop and test the new conductors and learn the design and manufacturing concepts needed for them. The flexible nature of a rapid turnaround program required the development of a standard coil cassette for different size cable, allowing coils to be used as building blocks for testing in different magnet configurations. Careful attention is given to the design of the coil structure: The inner bobbin the wire is wound on, the coil winding process, insulation integrity, epoxy vacuum impregnation, and final assembly into a test magnet. This paper will discuss the manufacturing techniques and design rules learned from the rapid turnaround program, and test results to date.

  20. Dollo's law and the re-evolution of shell coiling.

    PubMed Central

    Collin, Rachel; Cipriani, Roberto

    2003-01-01

    Gastropods have lost the quintessential snail feature, the coiled shell, numerous times in evolution. In many cases these animals have developed a limpet morphology with a cap-shaped shell and a large foot. Limpets thrive in marginal habitats such as hydrothermal vents, the high-energy rocky intertidal areas and fresh water, but they are considered to be evolutionary dead-ends, unable to re-evolve a coiled shell and therefore unable to give rise to the diversity seen among coiled snails. The re-evolution of a coiled shell, or any complex character, is considered unlikely or impossible (Dollo's law) because the loss of the character is followed by the loss of the genetic architecture and developmental mechanisms that underlie that character. Here, we quantify the level of coiling in calyptraeids, a family of mostly uncoiled limpets, and show that coiled shells have re-evolved at least once within this family. These results are the first demonstration, to our knowledge, of the re-evolution of coiling in a gastropod, and show that the developmental features underlying coiling have not been lost during 20-100 Myr of uncoiled evolutionary history. This is the first example of the re-evolution of a complex character via a change in developmental timing (heterochrony) rather than a change in location of gene expression (heterotopy). PMID:14728776

  1. High voltage fault current limiter having immersed phase coils

    SciTech Connect

    Darmann, Francis Anthony

    2014-04-22

    A fault current limiter including: a ferromagnetic circuit formed from a ferromagnetic material and including at least a first limb, and a second limb; a saturation mechanism surrounding a limb for magnetically saturating the ferromagnetic material; a phase coil wound around a second limb; a dielectric fluid surrounding the phase coil; a gaseous atmosphere surrounding the saturation mechanism.

  2. Functions for detecting malposition of transcutaneous energy transmission coils.

    PubMed

    Ozeki, Toshinaga; Chinzei, Tsuneo; Abe, Yusuke; Saito, Itsuro; Isoyama, Takashi; Mochizuki, Shuuichi; Ishimaru, Mitsuhiko; Takiura, Koki; Baba, Atsushi; Toyama, Takahiro; Imachi, Kou

    2003-01-01

    A transcutaneous energy transmission system (TETS) for artificial hearts and ventricular assist devices uses electrical coupling of power between external and implanted coils. If the position of coils changes relative to each other, the TETS cannot feed the required power of the implanted device. During activity or sleep, the coils may move accidentally. TETS users and the people around them have to pay attention to this because the range of the position where the required power can be fed efficiently is not wide. Therefore, we added functions for the position changes of the coils to the TETS. Regular, cautious, and irregular positions were introduced, and the ranges of them were decided upon in our experiments. The cautious position was determined by the area where the change of the relative position of the coils was relatively small. When the coils were in the cautious position, the circuit was tuned by way of changing the resonant point. This modulation could give good power efficiency in the cautious position. When the coils were in the irregular position, an alarm switch was turned on. These functions ease the restriction of the coil position and give better quality of life (QOL) than do the conventional TETS. PMID:12918593

  3. Coiled-Coils at the Edge of Configurational Heterogeneity. Structural Analyses of Parallel and Antiparallel Homotetrameric Coiled-Coils Reveal Configurational Sensitivity to a Single Solvent-Exposed Amino Acid Substitution.†§

    PubMed Central

    Yadav, Maneesh K.; Leman, Luke J.; Price, Daniel J.; Brooks, Charles L.; Stout, C. David; Ghadiri, M. Reza

    2007-01-01

    A detailed understanding of the mechanisms by which particular amino acid sequences can give rise to more than one folded structure, such as for proteins that undergo large conformational changes or misfolding, is a long-standing objective of protein chemistry. Here we describe the crystal structures of a single coiled-coil peptide in distinct parallel and antiparallel tetrameric configurations and further describe the parallel or antiparallel crystal structures of several related peptide sequences; the antiparallel tetrameric assemblies represents the first crystal structures of GCN4-derived peptides exhibiting such a configuration. Intriguingly, substitution of a single solvent-exposed residue enabled the parallel coiled-coil tetramer GCN4-pLI to populate the antiparallel configuration, suggesting that the two configurations are close enough in energy for subtle sequence changes to have important structural consequences. We present a structural analysis of the small changes to helix register and side chain conformations that accommodate the two configurations, and have supplemented these results using solution studies and a molecular dynamics energetic analysis using a replica exchange methodology. Considering the previous examples of structural nonspecificity in coiled-coil peptides, the findings reported here not only emphasize the predisposition of the coiled-coil motif to adopt multiple configurations, but also call attention to the associated risk that observed crytstal structures may not represent the only (or even the major) species present in solution. PMID:16584182

  4. Asymmetric zonal shim coils for magnetic resonance applications.

    PubMed

    Forbes, L K; Crozier, S

    2001-08-01

    A method is presented for the systematic design of asymmetric zonal shim coils for magnetic resonance applications. Fourier-series methods are used to represent the magnetic field inside and outside a circular cylinder of length 2L and radius a. The current density on the cylinder is also represented using Fourier series. Any desired field can be specified in advance on the cylinder's radius, over some nonsymmetric portion pLcoil's length (-1coil and the magnetic field components then follow automatically. The method is illustrated by applying it to three sample zonal coil designs, namely, linear, quadratic, and cubic fields located asymmetrically in the coil. Current densities and corresponding coil winding patterns are shown for these three illustrative cases. Field calculations directly from the coil patterns and spherical harmonic deconvolutions of these fields indicate that the example designs match the theory well. Asymmetric shim coils can be used in conventional symmetric MRI magnets, particularly those architected for "head-only" studies. One of their major applications is expected to be in the newly developed asymmetric magnet systems. PMID:11548933

  5. Transverse low-field RF coils in MRI.

    PubMed

    Claasen-Vujcić, T; Borsboom, H M; Gaykema, H J; Mehlkopf, T

    1996-07-01

    Imaging at low fields imposes a number of nonstandard requirements on the RF coil. At low fields, coil losses are dominant over patient losses. This means that even more stress is put on the quality factor Q. Furthermore, the low frequency also implies a high inductance L and/or a high capacitance C product. Just increasing the capacitance C results in a difficult optimal matching to the preamplifier as well as increased costs and higher complexity of the resonator construction. Coils with a high quality factor Q and a high inductance are thus required at low fields. Birdcage coils possess a number of advantages over saddle and solenoidal coils. However, the currently used birdcages have inherently low inductances limited by the size of the coil. The problem can be solved by a novel design in which the strip configuration for inductors is abandoned and the inductors are realized as a certain number of turns. The Q factor can be further improved by using Litz wire. Three novel transverse RF coils with high inductances are presented and compared with each other as well as to the standard coils. Both linear and quadrature modes are discussed. PMID:8795029

  6. Coil planet centrifugation as a means for small particle separation

    NASA Technical Reports Server (NTRS)

    Herrmann, F. T.

    1983-01-01

    The coil planet centrifuge uses a centrifugal force field to provide separation of particles based on differences in sedimentation rates by flow through a rotating coiled tube. Three main separations are considered: (1) single phase fresh sheep and human erythrocytes, (2) single phase fixed heep and human erythrocytes, and (3) electrophoretically enhanced single phase fresh sheep and human erythrocytes.

  7. Gelatinous Fibers are Widespread in Coiling Tendrils and Twining Vines

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Although tendrils coil and vines twine has been investigated since Darwin’s time, a full understanding of the mechanism(s) of tendril coiling and twining of vines has not yet been accomplished. In a previous study, it was observed that in tendrils of redvine, gelatinous fibers occurred concomitantl...

  8. Bipolar electrical coil based on YBCO bulks: initial tests

    NASA Astrophysics Data System (ADS)

    Alvarez, A.; Suárez, P.; Ceballos, J. M.; Pérez, B.; Werfel, F.; Floegel-Delor, U.

    2008-02-01

    In the field of the application of HTS in electrical motors, most prototypes are made using superconducting coils based on tape and located in the position where copper coils work in a similar conventional motor. Other prototypes use superconducting bulks (usually disk-shaped) in those positions where normal magnets should work in similar conventional motors. But it is very unusual to find designs using electrical coils based on bulks. This is a challenge whose main problem is the difficulty in machining the superconductor bulks to get the proper shape because of the impossibility of bending the material to wind coils. The design of a bipolar single-turn coil made from a superconducting YBCO disk was proposed by the group of Electrical Application of Superconductors, at the University of Extremadura, several years ago to be an element for the design of a modular two-phase inductor for an air core axial-flux motor. The shape of each coil looks like an 'S'. When a current flows through the circuit, two opposite magnetic fields appear in the upper and lower halves of the piece. Until now, attempts to get a good superconducting circuit by cutting a YBCO disk into the required shape have failed because of cracks appearing in the crystal during the process. Last year, our group at the University of Extremadura began to work with ATZ GmbH who have improved the machining process and made the coils. In this paper we present the coil and the first tests carried out.

  9. 13. ROOM 40, LOOKING WEST FROM THE RUBENS COIL. SPINNER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. ROOM 40, LOOKING WEST FROM THE RUBENS COIL. SPINNER MAGNETOMETER IS VISIBLE AT FAR LEFT, AND THE RUBENS COIL CONTROL UNIT AT FAR RIGHT. - U.S. Geological Survey, Rock Magnetics Laboratory, 345 Middlefield Road, Menlo Park, San Mateo County, CA

  10. Performance correlation between YBa2Cu3O7‑δ coils and short samples for coil technology development

    NASA Astrophysics Data System (ADS)

    Wang, X.; Dietderich, D. R.; Godeke, A.; Gourlay, S. A.; Marchevsky, M.; Prestemon, S. O.; Sabbi, G. L.

    2016-06-01

    A robust fabrication technology is critical to achieve the high performance in YBa2Cu3O{}7-δ (YBCO) coils as the critical current of the brittle YBCO layer is subject to the strain-induced degradation during coil fabrication. The expected current-carrying capability of the magnet and its temperature dependence are two key inputs to the coil technology development. However, the expected magnet performance is not straightforward to determine because the short-sample critical current depends on both the amplitude and orientation of the applied magnetic field with respect to the broad surface of the tape-form conductor. In this paper, we present an approach to calculate the self-field performance limit for YBCO racetrack coils at 77 and 4.2 K. Critical current of short YBCO samples was measured as a function of the applied field perpendicular to the conductor surface from 0 to 15 T. This field direction limited the conductor critical current. Two double-layer racetrack coils, one with three turns and the other with 10 turns, were wound and tested at 77 and 4.2 K. The test coils reached at least 80% of the expected critical current. The ratio between the coil critical currents at 77 and 4.2 K agreed well with the calculation. We conclude that the presented approach can determine the performance limit in YBCO racetrack coils based on the short-sample critical current and provide a useful guideline for assessing the coil performance and fabrication technology. The correlation of the coil critical current between 77 K and 4.2 K was also observed, allowing the 77 K test to be a cost-effective tool for the development of coil technology.

  11. Transformer current sensor for superconducting magnetic coils

    DOEpatents

    Shen, Stewart S.; Wilson, C. Thomas

    1988-01-01

    A transformer current sensor having primary turns carrying a primary current for a superconducting coil and secondary turns only partially arranged within the primary turns. The secondary turns include an active winding disposed within the primary turns and a dummy winding which is not disposed in the primary turns and so does not experience a magnetic field due to a flow of current in the primary turns. The active and dummy windings are wound in opposite directions or connected in series-bucking relationship, and are exposed to the same ambient magnetic field. Voltages which might otherwise develop in the active and dummy windings due to ambient magnetic fields thus cancel out. The resultant voltage is purely indicative of the rate of change of current flowing in the primary turns.

  12. Designing Magnetic Coils From the Inside Out

    NASA Astrophysics Data System (ADS)

    Wagner, Daniel

    2011-10-01

    Traditionally the design cycle for magnetic fields involves guessing at a reasonable conductor and magnetic material configuration, using finite element analysis (FEA) software to calculate the resulting field, modifying the configuration, and iterating to produce the desired results. We take the opposite approach of specifying the required magnetic field, imposing it as a boundary condition on the region of interest, and then solving the Laplace equation to determine the field outside that region. The exact conductor configuration along the boundaries is extracted from the magnetic scalar potential in a trivial manner. This method is being applied to design a coils for the neutron EDM experiment, and an RF waveguide in a new design of a neutron resonant spin flipper for the n-3He experiment. Both experiments will run at the Spallation Neutron Source (SNS) at Oak Ridge National Laboratory. Partially supported by the NSF under grant PHY-0855584.

  13. Design and optimization of efficient magnetic coils for biomedical applications

    NASA Astrophysics Data System (ADS)

    Ram Rakhyani, Anil Kumar

    Magnetic fields are permeable to the biological tissues and can induce electric field in the conductive structures. Some medical devices take advantage of this ability to transfer energy from the source to the receiving site without direct contact. Prosthetic devices such as retinal implants use time-varying magnetic field to achieve wireless power transfer to the implanted magnetic coil. However, devices such as magnetic stimulators use the induction principle to create an electric field at the stimulation site. Efficiency of these devices is primarily dependent on the design of the magnetic coils. Therefore, in this work, we designed and validated efficient magnetic coils for wireless power transfer to implanted devices and magnetic stimulation of the peripheral nerves. Typical wireless power transfer (WPT) systems uses two-coil based design to achieve contactless power transfer to the implanted electronics. These systems achieve low power transfer efficiency (< 30%) and frequency bandwidth. Moreover, efficient wireless system requires high coupling and load variation tolerance during device operation. To design an electromagnetic safe WPT system, the power absorbed by the tissue and radiated field due to the proximal magnetic coils needs to be minimized. In this work, we proposed a multi-coil power transfer system which solves some of the current challenges. The proposed multi-coil WPT system achieves more than twice the power transfer efficiency, controllable voltage gain, wider frequency bandwidth, higher tolerance to coupling and load variations, lower absorbed power in the tissue and lower radiated field from the magnetic coil than a comparable two-coil system. In this work, we have developed analytic models of the multi-coil WPT system and validated the accuracy of the solutions using experiments. Magnetic coils play an important role in controlling the distribution of induced electric field inside the nerve during magnetic stimulation. In the past

  14. Design and Analysis of the ITER Vertical Stability Coils

    SciTech Connect

    Peter H. Titus, et. al.

    2012-09-06

    The ITER vertical stability (VS) coils have been developed through the preliminary design phase by Princeton Plasma Physics Laboratory (PPPL). Final design, prototyping and construction will be carried out by the Chinese Participant Team contributing lab, Institute of Plasma Physics, Chinese Academy of Sciences (ASIPP). The VS coils are a part of the in-vessel coil systems which include edge localized mode (ELM) coils as well as the VS coils. An overview of the ELM coils is provided in another paper at this conference. 15 The VS design employs four turns of stainless steel jacketed mineral insulated copper (SSMIC) conductors The mineral insulation is Magnesium Oxide (MgO). Joule and nuclear heat is removed by water flowing at 3 m/s through the hollow copper conductor. A key element in the design is that slightly elevated temperatures in the conductor and its support spine during operation impose compressive stresses that mitigate fatigue damage. Away from joints, and break-outs, conductor thermal stresses are low because of the axisymmetry of the winding (there are no corner bends as in the ELM coils).The 120 degree segment joint, and break-out or terminal regions are designed with similar but imperfect constraint compared with the ring coil portion of the VS. The support for the break-out region is made from a high strength copper alloy, CuCrZr. This is needed to conduct nuclear heat to the actively cooled conductor and to the vessel wall. The support "spine" for the ring coil portion of the VS is 316 stainless steel, held to the vessel with preloaded 718 bolts. Lorentz loads resulting from normal operating loads, disruption loads and loads from disruption currents in the support spine shared with vessel, are applied to the VS coil. The transmission of the Lorentz and thermal expansion loads from the "spine" to the vessel rails is via friction augmented with a restraining "lip" to ensure the coil frictional slip is minimal and acceptable. Stresses in the coil

  15. Finding the Golgi: Golgin Coiled-Coil Proteins Show the Way.

    PubMed

    Gillingham, Alison K; Munro, Sean

    2016-06-01

    The Golgi apparatus lies at the centre of the secretory pathway. It consists of a series of flattened compartments typically organised into a stack that, in mammals, is connected to additional stacks to form a Golgi ribbon. The Golgi is responsible for the maturation and modification of proteins and lipids, and receives and exports vesicles to and from multiple destinations within the cell. This complex trafficking network requires that only the correct vesicles fuse with the correct destination membrane. Recently, a group of coiled-coil proteins called golgins were shown to not only capture incoming vesicles but to also provide specificity to the tethering step. This raises many interesting questions about how they interact with other components of membrane traffic, some of which may also contribute to specificity. PMID:26972448

  16. Gene Delivery from Supercharged Coiled-coil Protein and Cationic Lipid Hybrid Complex

    PubMed Central

    More, Haresh T.; Frezzo, Joseph A.; Dai, Jisen; Yamano, Seiichi; Montclare, Jin K.

    2014-01-01

    A lipoproteoplex comprised of an engineered supercharged coiled-coil protein (CSP) bearing multiple arginines and the cationic lipid formulation FuGENE HD (FG) was developed for effective condensation and delivery of nucleic acids. The CSP was able to maintain helical structure and self-assembly properties while exhibiting binding to plasmid DNA. The ternary CSP•DNA(8:1)•FG lipoproteoplex complex demonstrated enhanced transfection of β-galactosidase DNA into MC3T3-E1 mouse preosteoblasts. The lipoproteoplexes showed significant increases in transfection efficiency when compared to conventional FG and an mTat•FG lipopolyplex with a 6- and 2.5-fold increase in transfection, respectively. The CSP•DNA(8:1)•FG lipoproteoplex assembled into spherical particles with a net positive surface charge, enabling efficient gene delivery. These results support the application of lipoproteoplexes with protein engineered CSP for non-viral gene delivery. PMID:24875765

  17. Interactive simulation of embolization coils: modeling and experimental validation.

    PubMed

    Dequidt, Jérémie; Marchal, Maud; Duriez, Christian; Kerien, Erwan; Cotin, Stéphane

    2008-01-01

    Coil embolization offers a new approach to treat aneurysms. This medical procedure is namely less invasive than an open-surgery as it relies on the deployment of very thin platinum-based wires within the aneurysm through the arteries. When performed intracranially, this procedure must be particularly accurate and therefore carefully planned and performed by experienced radiologists. A simulator of the coil deployment represents an interesting and helpful tool for the physician by providing information on the coil behavior. In this paper, an original modeling is proposed to obtain interactive and accurate simulations of coil deployment. The model takes into account geometric nonlinearities and uses a shape memory formulation to describe its complex geometry. An experimental validation is performed in a contact-free environment to identify the mechanical properties of the coil and to quantitatively compare the simulation with real data. Computational performances are also measured to insure an interactive simulation. PMID:18979807

  18. Impedance of curved rectangular spiral coils around a conductive cylinder

    NASA Astrophysics Data System (ADS)

    Burke, S. K.; Ditchburn, R. J.; Theodoulidis, T. P.

    2008-07-01

    Eddy-current induction due to a thin conformable coil wrapped around a long conductive cylinder is examined using a second-order vector potential formalism. Compact closed-form expressions are derived for the self- and mutual impedances of curved rectangular spiral coils (i) in free space and (ii) when wrapped around the surface of the cylindrical rod. The validity of these expressions was tested against the results of a systematic series of experiments using a cylindrical Al-alloy rod and conformable coils manufactured using flexible printed-circuit-board technology. The theoretical expressions were in very good agreement with the experimental measurements. The significance of the results for eddy-current nondestructive inspection using flexible coils and flexible coil arrays is discussed.

  19. New head gradient coil design and construction techniques

    PubMed Central

    Handler, William B; Harris, Chad T; Scholl, Timothy J; Parker, Dennis L; Goodrich, K Craig; Dalrymple, Brian; Van Sass, Frank; Chronik, Blaine A

    2013-01-01

    Purpose To design and build a head insert gradient coil to use in conjunction with body gradients for superior imaging. Materials and Methods The use of the Boundary Element Method to solve for a gradient coil wire pattern on an arbitrary surface has allowed us to incorporate engineering changes into the electromagnetic design of a gradient coil directly. Improved wire pattern design has been combined with robust manufacturing techniques and novel cooling methods. Results The finished coil had an efficiency of 0.15 mT/m/A in all three axes and allowed the imaging region to extend across the entire head and upper part of the neck. Conclusion The ability to adapt your electromagnetic design to necessary changes from an engineering perspective leads to superior coil performance. PMID:24123485

  20. Magnet coil system for a superconducting spectrometer (HISS)

    SciTech Connect

    Reimers, R.M.; Wolgast, R.C.; Yamamoto, R.M.

    1983-03-01

    The Heavy Ion Superconducting Spectrometer (HISS) facility and coils are briefly described while most of the paper discusses the support structures consisting of flanged doubly tapered stainless steel cylinders having a Z-shaped cross-section with average diameter of approximately 2.35 meters and height of approx. .49 meters. This member serves as a one piece coil support to resist gravitational, seismic, and magnetic forces with an approximate heat leak to helium of 59 watts per cylinder during operation of magnet at 4 degrees K. Maximum magnetic forces on each coil are over 11 meganewtons at 3T excitation. Magnetic forces attracting the coils to the core vary with excitation in direction as well as in magnitude. Radial magnetic forces produce stresses internal to the coil bobbin.

  1. TEST PLAN FOR MONITORING COOLING COILS IN A LABORATORY SETTING

    SciTech Connect

    Don B. Shirey, III

    2002-04-01

    The objective of this research project is to understand and quantify the moisture removal performance of cooling coils at part-load conditions. The project will include a comprehensive literature review, detailed measurement of cooling coil performance in a laboratory facility, monitoring cooling systems at several field test sites, and development/validation of engineering models that can be used in energy calculations and building simulations. This document contains the detailed test plan for monitoring cooling coil performance in a laboratory setting. Detailed measurements will be taken on up to 10 direct expansion (DX) and chilled water cooling coils in various configurations to understand the impact of coil geometry and operating conditions on transient moisture condensation and evaporation.

  2. Lessons Learned During the Manufacture of the NCSX Modular Coils

    SciTech Connect

    James H. Chrzanowski,Thomas G. Meighan, Steven Raftopoulos and Lawrence Dudek and Paul J. Fogarty

    2009-09-15

    The National Compact Stellarator Experiment's (NCSX) modular coils presented a number of engineering and manufacturing challenges due to their complex shapes, requirements for high dimensional accuracy and high current density requirements due to space constraints. Being the first of their kind, these coils required the implementation of many new manufacturing and measuring techniques and procedures. This was the first time that these manufacturing techniques and methods were applied in the production of coils at the laboratory. This resulted in a steep learning curve for the first several coils. Through the effective use of procedures, tooling modifications, involvement and ownership by the manufacturing workforce, and an emphasis on safety, the assembly team was able to reduce the manufacturing times and improve upon the manufacturing methods. This paper will discuss the learning curve and steps that were taken to improve the manufacturing efficiency and reduce the manufacturing times for the modular coils without forfeiting quality.

  3. Vehicle to wireless power transfer coupling coil alignment sensor

    DOEpatents

    Miller, John M.; Chambon, Paul H.; Jones, Perry T.; White, Clifford P.

    2016-02-16

    A non-contacting position sensing apparatus includes at least one vehicle-mounted receiver coil that is configured to detect a net flux null when the vehicle is optimally aligned relative to the primary coil in the charging device. Each of the at least one vehicle-mounted receiver coil includes a clockwise winding loop and a counterclockwise winding loop that are substantially symmetrically configured and serially connected to each other. When the non-contacting position sensing apparatus is located directly above the primary coil of the charging device, the electromotive forces from the clockwise winding loop and the counterclockwise region cancel out to provide a zero electromotive force, i.e., a zero voltage reading across the coil that includes the clockwise winding loop and the counterclockwise winding loop.

  4. Rapid mixing of viscous liquids by electrical coiling

    NASA Astrophysics Data System (ADS)

    Kong, Tiantian; Li, Jingmei; Liu, Zhou; Wang, Liqiu; Shum, Ho Cheung

    2015-11-01

    We study the coiling of viscous liquid jets under an axial electric field. As a viscous jet accelerated by the electric field encounters a solid substrate, it is forced to decelerate, leading a compressive force that sets the jet to coil. We show that the coiling characteristics are significantly influenced by the applied electric force. Based on a balance between the electric and viscous torque, we deduce a scaling law to predict the coiling frequency from the relevant physical parameters, including the viscosity, dielectric constant, volumetric flow rate of the liquid and the applied electric field intensity. Moreover, we exploit this electrically controlled coiling to achieve rapid mixing between viscous liquids. We show that as a compound viscous jet is induced to coil electrically, the diffusion distance between viscous liquids is significantly reduced. As such, the mixing is enhanced remarkably despite the low Reynolds number, which is on the order of 10-7. We further show that the degree of mixing can be precisely tuned by the applied electric force. Our approach of electric coiling offers a novel and versatile way to dispense, mix and print precursor liquids with large viscosities, including resins, food suspensions and polymer blends.

  5. Discrete-coil investigations of modular stellarator configurations

    SciTech Connect

    Lilliequist, C.G.

    1985-10-01

    The existence of a vacuum magnetic well is generally accepted as a prerequisite to start-up. The special set of modular-stellarator configurations selected for the present computational investigation was derived from coefficients and equations that produced finite-beta wells in a continuous-current-sheet representation. The corresponding coils did not produce magnetic wells in a vacuum when their field configuration was investigated with a discrete-coil code. Vacuum magnetic wells have been identified through the use of this discrete-coil code in previous Heliac and stellarator studies. Therefore, these finite-beta magnetic wells could be the consequence of the continuous-current-sheet model: a conjecture that is supported by the linear scaling of the magnetic hills found in the present work as a function of the separation between the coils used. In addition to magnetic field profiles, comparisons are shown here of ripple, rotational transform, and flux-surface shapes for the discrete-coil, modular stellarators under study. Initially, each of the significant parameters affecting the shape of the control surface upon which the coils lie and the deformation of the individual coils was varied separately in search of a vacuum magnetic well in the parameter neighborhood of the successful finite-beta configuration.

  6. Single-coil magnetic induction tomographic three-dimensional imaging

    PubMed Central

    Feldkamp, Joseph R.

    2015-01-01

    Abstract. Previously, magnetic induction tomography (MIT) has been considered for noncontact imaging of human tissue electrical properties. Commonly, multiple coils are used, with any one serving as the source while others detect eddy currents generated in the specimen. Here, imaging of low conductivity objects is shown feasible with a single coil acting simultaneously as source and detector, provided that the coil is repeatedly relocated while collecting coil loss data. To enable such “scanning,” an analytical coil loss formula is derived in the quasistatic limit for a single coil consisting of several concentric circular wire loops, all within a common plane. Conductivity may vary arbitrarily in space, whereas permittivity and permeability are treated as uniform. The analytical form is used to build an algorithm for imaging electrical conductivity in human tissues. A practical device operating at 12.5 MHz is described and used in a clinical trial that “scans” the region between the scapulae while collecting coil loss data. Inversion of data leads to electrical conductivity distribution images for the thoracic spinal column which are the first of their kind to correctly distinguish such basic features as size and depth of spinal canal, as well as size, depth, and spacing of transverse spinal processes. PMID:26158078

  7. Fetal MRI on a multi-element digital coil platform.

    PubMed

    Serai, Suraj D; Merrow, Arnold C; Kline-Fath, Beth M

    2013-09-01

    Fetal MRI has an increasing list of indications and is most commonly employed when anomalies detected by prenatal ultrasonography require further characterization. This may occur when sonography is technically challenging or where specific MRI findings will determine pre- and postnatal management, including critical in utero and/or peripartum interventions. In these circumstances, there are high expectations for MRI to sort out complex diagnostic dilemmas through exquisite anatomical imaging that fetal surgeons and obstetricians can comprehend and relay to their patients. These expectations, in light of evolving clinical innovations, continue to drive advances in fetal imaging. Increasing signal-to-noise ratio (SNR) is fundamental to improving MR image quality, and proper coil selection is a key component of this pursuit. Since the introduction of parallel imaging techniques, the numbers of elements in phased-array coils have been continuously increased to achieve high SNR and shorter scan times. With the invention of a digital coil platform, it is now possible to connect combinations of multiple coil elements to enhance SNR beyond the capabilities of the adult eight-channel torso-coil routinely used in fetal imaging. This paper describes the application of multi-element radiofrequency coils on a digital broadband imaging platform with unique coil combinations to perform dedicated fetal MRI. PMID:23649206

  8. Axisymmetric Simulations of the ITER Vertical Stability Coil

    SciTech Connect

    Titus, Peter H.

    2013-07-09

    The ITER in-vessel coil system includes Vertical Stability (VS) coils and Edge Localized Mode (ELM) coils. There are two large VS ring coils, one upper and one lower. Each has four turns which are independently connected. The VS coils are needed for successful operation of ITER for most all of its operating modes. The VS coils must be highly reliable and fault tolerant. The operating environment includes normal and disruption Lorentz forces. To parametrically address all these design conditions in a tractable analysis requires a simplified model. The VS coils are predominately axisymmetric, and this suggests that an axisymmetric model can be meaningfully used to address the variations in mechanical design, loading, material properties, and time dependency. The axisymmetric finite element analysis described in this paper includes simulations of the bolted frictional connections used for the mounting details. Radiation and elastic-plastic response are modeled particularly for the extreme faulted conditions. Thermal connectivity is varied to study the effects of partial thermal connection of the actively cooled conductor to the remaining structure.

  9. Manufacturing Development of the NCSX Modular Coil Windings

    SciTech Connect

    Chrzanowsk, J. H.; Fogarty, P. J.; Heitzenroeder, P. J.; Meighan, T.; Nelson, B.; Raftopoulos, S.; Williamson, D.

    2005-09-27

    The modular coils on the National Compact Stellarator Experiment (NCSX) present a number of significant engineering challenges due to their complex shapes, requirements for high dimensional accuracy and the high current density required in the modular coils due to space constraints. In order to address these challenges, an R&D program was established to develop the conductor, insulation scheme, manufacturing techniques, and procedures. A prototype winding named Twisted Racetrack Coil (TRC) was of particular importance in dealing with these challenges. The TRC included a complex shaped winding form, conductor, insulation scheme, leads and termination, cooling system and coil clamps typical of the modular coil design. Even though the TRC is smaller in size than a modular coil, its similar complex geometry provided invaluable information in developing the final design, metrology techniques and development of manufacturing procedures. In addition a discussion of the development of the copper rope conductor including "Keystoning" concerns; the epoxy impregnation system (VPI) plus the tooling and equipment required to manufacture the modular coils will be presented.

  10. Focusing and targeting of magnetic brain stimulation using multiple coils.

    PubMed

    Ruohonen, J; Ilmoniemi, R J

    1998-05-01

    Neurones can be excited by an externally applied time-varying electromagnetic field. Focused magnetic brain stimulation is attained using multiple small coils instead of one large coil, the resultant induced electric field being a superposition of the fields from each coil. In multichannel magnetic brain stimulation, partial cancellation of fields from individual coils provides a significant improvement in the focusing of the stimulating field, and independent coil channels allow targeting of the stimuli on a given spot without moving the coils. The problem of shaping the stimulating field in multichannel stimulation is analysed, and a method is derived that yields the driving currents required to induce a field with a user-defined shape. The formulation makes use of lead fields and minimum-norm estimation from magneto-encephalography. Using these methods, some properties of multichannel coil arrays are examined. Computer-assisted multichannel stimulation of the cortex will enable several new studies, including quick determination of the cortical regions, the stimulation of which disrupts cortical processing required by a task. PMID:9747568

  11. AC Loss Measurements on a 2G YBCO Coil

    SciTech Connect

    Rey, Christopher M; Duckworth, Robert C; Schwenterly, S W

    2011-01-01

    The Oak Ridge National Laboratory (ORNL) is collaborating with Waukesha Electric Systems (WES) to continue development of HTS power transformers. For compatibility with the existing power grid, a commercially viable HTS transformer will have to operate at high voltages in the range of 138 kV and above, and will have to withstand 550-kV impulse voltages as well. Second-generation (2G) YBCO coated conductors will be required for an economically-competitive design. In order to adequately size the refrigeration system for these transformers, the ac loss of these HTS coils must be characterized. Electrical AC loss measurements were conducted on a prototype high voltage (HV) coil with co-wound stainless steel at 60 Hz in a liquid nitrogen bath using a lock-in amplifier technique. The prototype HV coil consisted of 26 continuous (without splice) single pancake coils concentrically centered on a stainless steel former. For ac loss measurement purposes, voltage tap pairs were soldered across each set of two single pancake coils so that a total of 13 separate voltage measurements could be made across the entire length of the coil. AC loss measurements were taken as a function of ac excitation current. Results show that the loss is primarily concentrated at the ends of the coil where the operating fraction of critical current is the highest and show a distinct difference in current scaling of the losses between low current and high current regimes.

  12. Microhole Coiled Tubing Bottom Hole Assemblies

    SciTech Connect

    Don Macune

    2008-06-30

    The original objective of the project, to deliver an integrated 3 1/8-inch diameter Measurement While Drilling (MWD) and Logging While Drilling (LWD) system for drilling small boreholes using coiled tubing drilling, has been achieved. Two prototype systems have been assembled and tested in the lab. One of the systems has been successfully tested downhole in a conventional rotary drilling environment. Development of the 3 1/8-inch system has also lead to development and commercialization of a slightly larger 3.5-inch diameter system. We are presently filling customer orders for the 3.5-inch system while continuing with commercialization of the 3 1/8-inch system. The equipment developed by this project will be offered for sale to multiple service providers around the world, enabling the more rapid expansion of both coiled tubing drilling and conventional small diameter drilling. The project was based on the reuse of existing technology whenever possible in order to minimize development costs, time, and risks. The project was begun initially by Ultima Labs, at the time a small company ({approx}12 employees) which had successfully developed a number of products for larger oil well service companies. In September, 2006, approximately 20 months after inception of the project, Ultima Labs was acquired by Sondex plc, a worldwide manufacturer of downhole instrumentation for cased hole and drilling applications. The acquisition provided access to proven technology for mud pulse telemetry, downhole directional and natural gamma ray measurements, and surface data acquisition and processing, as well as a global sales and support network. The acquisition accelerated commercialization through existing Sondex customers. Customer demand resulted in changes to the product specification to support hotter (150 C) and deeper drilling (20,000 psi pressure) than originally proposed. The Sondex acquisition resulted in some project delays as the resistivity collar was interfaced to a

  13. Self-assembly of cyclic rod-coil diblock copolymers.

    PubMed

    He, Linli; Chen, Zenglei; Zhang, Ruifen; Zhang, Linxi; Jiang, Zhouting

    2013-03-01

    The phase behavior of cyclic rod-coil diblock copolymer melts is investigated by the dissipative particle dynamics simulation. In order to understand the effect of chain topological architecture better, we also study the linear rod-coil system. The comparison of the calculated phase diagrams between the two rod-coil copolymers reveals that the order-disorder transition point (χN)ODT for cyclic rod-coil diblock copolymers is always higher than that of equivalent linear rod-coil diblocks. In addition, the phase diagram for cyclic system is more "symmetrical," due to the topological constraint. Moreover, there are significant differences in the self-assembled overall morphologies and the local molecular arrangements. For example, frod = 0.5, both lamellar structures are formed while rod packing is different greatly in cyclic and linear cases. The lamellae with rods arranged coplanarly into bilayers occurs in cyclic rod-coil diblocks, while the lamellar structure with rods arranged end by end into interdigitated bilayers appears in linear counterpart. In both the lamellar phases, the domain size ratio of cyclic to linear diblocks is ranged from 0.63 to 0.70. This is attributed to that the cyclic architecture with the additional junction increases the contacts between incompatible blocks and prevents the coil chains from expanding as much as the linear cases. As frod = 0.7, the hexagonally packed cylinder is observed for cyclic rod-coil diblocks, while liquid-crystalline smectic A lamellar phase is formed in linear system. As a result, the cyclization of a linear rod-coil block copolymer can induce remarkable differences in the self-assembly behavior and also diversify its physical properties and applications greatly. PMID:23485326

  14. Exploring functional connectivity networks with multichannel brain array coils.

    PubMed

    Anteraper, Sheeba Arnold; Whitfield-Gabrieli, Susan; Keil, Boris; Shannon, Steven; Gabrieli, John D; Triantafyllou, Christina

    2013-01-01

    The use of multichannel array head coils in functional and structural magnetic resonance imaging (MRI) provides increased signal-to-noise ratio (SNR), higher sensitivity, and parallel imaging capabilities. However, their benefits remain to be systematically explored in the context of resting-state functional connectivity MRI (fcMRI). In this study, we compare signal detectability within and between commercially available multichannel brain coils, a 32-Channel (32Ch), and a 12-Channel (12Ch) at 3T, in a high-resolution regime to accurately map resting-state networks. We investigate whether the 32Ch coil can extract and map fcMRI more efficiently and robustly than the 12Ch coil using seed-based and graph-theory-based analyses. Our findings demonstrate that although the 12Ch coil can be used to reveal resting-state connectivity maps, the 32Ch coil provides increased detailed functional connectivity maps (using seed-based analysis) as well as increased global and local efficiency, and cost (using graph-theory-based analysis), in a number of widely reported resting-state networks. The exploration of subcortical networks, which are scarcely reported due to limitations in spatial-resolution and coil sensitivity, also proved beneficial with the 32Ch coil. Further, comparisons regarding the data acquisition time required to successfully map these networks indicated that scan time can be significantly reduced by 50% when a coil with increased number of channels (i.e., 32Ch) is used. Switching to multichannel arrays in resting-state fcMRI could, therefore, provide both detailed functional connectivity maps and acquisition time reductions, which could further benefit imaging special subject populations, such as patients or pediatrics who have less tolerance in lengthy imaging sessions. PMID:23510203

  15. REACTORS

    DOEpatents

    Spitzer, L. Jr.

    1961-10-01

    Thermonuclear reactors, methods, and apparatus are described for controlling and confining high temperature plasma. Main axial confining coils in combination with helical windings provide a rotational transform that avoids the necessity of a figure-eight shaped reactor tube. The helical windings provide a multipolar helical magnetic field transverse to the axis of the main axial confining coils so as to improve the effectiveness of the confining field by counteracting the tendency of the more central lines of force in the stellarator tube to exchange positions with the magnetic lines of force nearer the walls of the tube. (AEC)

  16. Determination of coil defrosting loads. Part 5: Analysis of loads

    SciTech Connect

    Al-Mutawa, N.K.; Sherif, S.A.

    1998-10-01

    This paper (Part 5) provides load analysis for a hot-gas defrosted finned-tube freezer coil for entering air dry-bulb temperatures of {minus}8 F and {minus}13 F. The load analysis covers the total refrigeration load rate, average load sensible heat ratio, defrost heat input, defrost efficiency, and other relevant parameters that lead to the determination of the heat loads due to coil hot-gas defrosting. The intent is to provide some insight into the energy penalty associated with defrosting these types of coils using the hot-gas refrigerant method.

  17. Magnetic mirror structure for testing shell-type quadrupole coils

    SciTech Connect

    Andreev, N.; Barzi, E.; Bossert, R.; Chlachidze, G.; Kashikhin, V.S.; Kashikhin, V.V.; Lamm, M.J.; Nobrega, F.; Novitski, I.; Tartaglia, N.; Turrioni, D.; /Fermilab

    2009-10-01

    This paper presents magnetic and mechanical designs and analyses of the quadrupole mirror structure to test single shell-type quadrupole coils. Several quadrupole coils made of different Nb{sub 3}Sn strands, cable insulation and pole materials were tested using this structure at 4.5 and 1.9 K. The coils were instrumented with voltage taps, spot heaters, temperature sensors and strain gauges to study their mechanical and thermal properties and quench performance. The results of the quadrupole mirror model assembly and test are reported and discussed.

  18. Parametric design of tri-axial nested Helmholtz coils

    SciTech Connect

    Abbott, Jake J.

    2015-05-15

    This paper provides an optimal parametric design for tri-axial nested Helmholtz coils, which are used to generate a uniform magnetic field with controllable magnitude and direction. Circular and square coils, both with square cross section, are considered. Practical considerations such as wire selection, wire-wrapping efficiency, wire bending radius, choice of power supply, and inductance and time response are included. Using the equations provided, a designer can quickly create an optimal set of custom coils to generate a specified field magnitude in the uniform-field region while maintaining specified accessibility to the central workspace. An example case study is included.

  19. Fabrication of a helical coil shape memory alloy actuator

    SciTech Connect

    O`Donnell, R.E.

    1992-02-01

    A fabrication process was developed to form, heat treat, and join NiTi shape memory alloy helical coils for use as mechanical actuators. Tooling and procedures were developed to wind both extension and compression-type coils on a manual lathe. Heat treating fixtures and techniques were used to set the ``memory`` of the NiTi alloy to the desired configuration. A swaging process was devised to fasten shape memory alloy extension coils to end fittings for use in actuator testing and for potential attachment to mechanical devices. The strength of this mechanical joint was evaluated.

  20. Fabrication of a helical coil shape memory alloy actuator

    SciTech Connect

    O'Donnell, R.E.

    1992-02-01

    A fabrication process was developed to form, heat treat, and join NiTi shape memory alloy helical coils for use as mechanical actuators. Tooling and procedures were developed to wind both extension and compression-type coils on a manual lathe. Heat treating fixtures and techniques were used to set the memory'' of the NiTi alloy to the desired configuration. A swaging process was devised to fasten shape memory alloy extension coils to end fittings for use in actuator testing and for potential attachment to mechanical devices. The strength of this mechanical joint was evaluated.

  1. Fabrication of miniature optical fiber and microfiber coils

    NASA Astrophysics Data System (ADS)

    Sumetsky, M.; Dulashko, Y.; Ghalmi, S.

    2010-03-01

    We demonstrate a technique for simultaneous drawing, tapering, and coiling of a silica optical fiber that is locally heated by a CO 2 laser. It allows us to fabricate coils of silica fibers and microfibers with diameters ranging from ˜100 μm down to less than a micron. As an application, we demonstrate an evanescent coiled fiber refractometric sensor comprising 15 cm of standard single mode optical fiber. The dimension of this sensor is only 3 mm×3 mm×4 mm and its sensitivity in the aqueous environment is estimated as ˜160 nm/RIU.

  2. Investigation of the mechanical properties of superconducting coils

    SciTech Connect

    Markely, F.W.; Kerby, J.S.

    1990-03-01

    This paper presents data on 3 of the important mechanical properties of SSC type superconducting coils. The measured properties are: (1) The azimuthal elastic modulus of the coil samples made for the stress relaxation tests. (2) The rate of stress --- relaxation of collared SSC outer coils molded to different sizes and (3) The pressures that various insulations can withstand during molding or collaring before turn-to-turn shorts develop. Additional data on these and other properties are available but omitted here because of space limitations. 3 refs., 10 figs.

  3. Stent-assisted coil embolization of coronary artery aneurysm.

    PubMed

    Terasawa, Akihiro; Yokoi, Tuyoshi; Kondo, Keita

    2013-08-01

    Coronary artery aneurysms are uncommon diseases with potential complications including rupture and ischemia from embolic events or thrombosis. No consensus has been established regarding the optimal therapy for coronary artery aneurysms. Percutaneous catheter-based treatments using membrane-covered stents and coil embolization have been described. However, only few reports of stent-assisted coil embolization for coronary artery aneurysms have been published to date. Therefore, we report a case of coronary artery aneurysm successfully treated with stent-assisted coil embolization. PMID:23913616

  4. The Role of Filtration in Maintaining Clean Heat Exchanger Coils

    SciTech Connect

    Li Yang; James E. Braun; Eckhard A. Groll

    2004-06-30

    The main purpose of the study was to investigate the role of filtration in maintaining clean heat exchanger coils and overall performance. Combinations of 6 different levels of filtration (MERV 14, 11, 8, 6, 4, and no filter) and 4 different coils (an eight-row lanced-fin coil, HX8L), (an eight-row wavy-fin coil, HX8W), (a four-row lanced-fin coil, HX4L) and (a two-row lanced-fin coil, HX2L) were tested at 4 different air velocities (1.52, 2.03, 2.54,3.05 m/s (300, 400, 500, 600 ft/min)). The fouled conditions were obtained after injection of 600 grams of ASHRAE standard dust upstream of the filter/coil combination. This magnitude of dust is representative of a year of normal operation for an air conditioning system. The air-side pressure drops of the coils and filters and air-side heat transfer coefficients of the coils were determined from the measurements under the clean and fouled conditions. Depending upon the filter and coil test, the coil pressure drops increased in the range of 6%-30% for an air velocity at 2.54 m/s (500 ft/min). The impact was significantly greater for tests performed without a filter. The largest relative effect of fouling on pressure drop occurs for coils with fewer rows and having lanced fins. Coils with a greater number of rows can hold more dust so that a fixed amount of dust has a relatively smaller impact. The impact of fouling on air-side heat transfer coefficients was found to be relatively small. In some cases, heat transfer was actually enhanced due to additional turbulence caused by the presence of dust. The experimental results for pressure drops and heat transfer coefficients were correlated and the correlations were implemented within computer models of prototypical rooftop air conditioners and used to evaluate the impact of fouling on cooling capacity and EER. The equipment cooling capacity is reduced with fouling primarily because of a decrease in air flow due to the increase pressure drop rather than due to changes in h

  5. Traveling wave instability in helical coil flow

    NASA Astrophysics Data System (ADS)

    Webster, D. R.; Humphrey, J. A. C.

    1997-02-01

    Complementary flow visualization photographs and numerical calculations are presented for the transitional state between the laminar and turbulent flow regimes in a helically coiled pipe. The flow visualization covers a Reynolds number range from 3800 to 8650 (890

  6. Advances in coiled-tubing operating systems

    SciTech Connect

    Sas-Jaworsky, A. II

    1997-06-01

    The expansion of coiled tubing (CT) applications into spooled flowlines, spooled completions, and CT drilling continues to grow at an accelerated rate. For many users within the oil and gas industry, the CT industry appears to be poised on the threshold of the next logical step in its evolution, the creation of a fully integrated operating system. However, for CT to evolve into such an operating system, the associated services must be robust and sufficiently reliable to support the needs of exploration, development drilling, completion, production management, and wellbore-retirement operations both technically and economically. The most critical hurdle to overcome in creating a CT-based operating system is a fundamental understanding of the operating scope and physical limitations of CT technology. The complete list of mechanisms required to advance CT into an operating system is large and complex. However, a few key issues (such as formal education, training, standardization, and increased levels of experience) can accelerate the transition. These factors are discussed.

  7. The Search-Coil Magnetometer for MMS

    NASA Astrophysics Data System (ADS)

    Le Contel, O.; Leroy, P.; Roux, A.; Coillot, C.; Alison, D.; Bouabdellah, A.; Mirioni, L.; Meslier, L.; Galic, A.; Vassal, M. C.; Torbert, R. B.; Needell, J.; Rau, D.; Dors, I.; Ergun, R. E.; Westfall, J.; Summers, D.; Wallace, J.; Magnes, W.; Valavanoglou, A.; Olsson, G.; Chutter, M.; Macri, J.; Myers, S.; Turco, S.; Nolin, J.; Bodet, D.; Rowe, K.; Tanguy, M.; de la Porte, B.

    2016-03-01

    The tri-axial search-coil magnetometer (SCM) belongs to the FIELDS instrumentation suite on the Magnetospheric Multiscale (MMS) mission (Torbert et al. in Space Sci. Rev. (2014), this issue). It provides the three magnetic components of the waves from 1 Hz to 6 kHz in particular in the key regions of the Earth's magnetosphere namely the subsolar region and the magnetotail. Magnetospheric plasmas being collisionless, such a measurement is crucial as the electromagnetic waves are thought to provide a way to ensure the conversion from magnetic to thermal and kinetic energies allowing local or global reconfigurations of the Earth's magnetic field. The analog waveforms provided by the SCM are digitized and processed inside the digital signal processor (DSP), within the Central Electronics Box (CEB), together with the electric field data provided by the spin-plane double probe (SDP) and the axial double probe (ADP). On-board calibration signal provided by DSP allows the verification of the SCM transfer function once per orbit. Magnetic waveforms and on-board spectra computed by DSP are available at different time resolution depending on the selected mode. The SCM design is described in details as well as the different steps of the ground and in-flight calibrations.

  8. Inspecting coiled tubing for well operations

    SciTech Connect

    Gard, M.F.; Pasternack, E.S.; Smith, L.J.

    1992-02-18

    This patent describes improvement in a coiled tubing system for insertion of a substantially continuous bendable length of metal tubing into and withdrawal from a wellbore, the system including a tubing injection unit disposed for injecting the length of tubing into the well bore and storage means for dispensing the length of tubing and receiving the length of tubing from the injection unit. The improvement includes: tubing inspection apparatus for substantially continuously inspecting the wall section of the tubing to detect cracks and structural defects which may lead to tubing failure, the apparatus comprising: a source of electromagnetic radiation mounted in proximity to the tubing between the injection unit and a wellhead into which the tubing is injected; a radiation detector unit for receiving signals from the source which have been projected through the wall of the tubing; means for receiving signals form the detector unit for monitoring the structural integrity o the wall of the tubing during one of injecting and withdrawing the tubing with respect to the wellhead; and housing means supported for rotation about a longitudinal axis of the tubing.

  9. ITER coils insulation R and D program

    SciTech Connect

    Vieira, R.F.; Sugimoto, M.; Osaki, O.; Fujioka, T.; Korsunsky, V.; Reed, R.P.; Katheder, H.; Broadbent, A.

    1995-12-31

    The ITER coil insulation systems are required for operating voltages up to 10 KV and to support shear and normal compression loads through the winding pack. Manufacturing considerations and nuclear radiation resistance also influence the choice of suitable systems. A screening program of candidate systems is being conducted in stages. The first stage is reported in this paper. Present R and D data based on small samples indicate a static shear strength of about 85 MPa for a glass or alumina and epoxy resin vacuum pressure impregnation system and 50 MPa for a pre-impregnated glass/epoxy resin system with polyimide interleaved at a temperature of 4K. The preliminary irradiation results show that coating the steel surface with inorganic materials such as Al{sub 2}O{sub 3} or ZrO{sub 2}/8 Y{sub 2}O{sub 3} is beneficial in increasing the radiation resistance as far as shear strength is concerned. In addition Tetrafunctional epoxy (TGDM) systems appear to be more radiation resistant than Diglycidyl Ether of Bisphenol-A (DGEBA) systems. Further R and D work will focus on prototypical samples to continue evaluation of the performance of the insulation systems.

  10. Balance between Coiled-Coil Stability and Dynamics Regulates Activity of BvgS Sensor Kinase in Bordetella

    PubMed Central

    Lesne, E.; Krammer, E.-M.; Dupre, E.; Locht, C.; Lensink, M. F.

    2016-01-01

    ABSTRACT The two-component system BvgAS controls the expression of the virulence regulon of Bordetella pertussis. BvgS is a prototype of bacterial sensor kinases with extracytoplasmic Venus flytrap perception domains. Following its transmembrane segment, BvgS harbors a cytoplasmic Per-Arnt-Sim (PAS) domain and then a predicted 2-helix coiled coil that precede the dimerization-histidine-phosphotransfer domain of the kinase. BvgS homologs have a similar domain organization, or they harbor only a predicted coiled coil between the transmembrane and the dimerization-histidine-phosphotransfer domains. Here, we show that the 2-helix coiled coil of BvgS regulates the enzymatic activity in a mechanical manner. Its marginally stable hydrophobic interface enables a switch between a state of great rotational dynamics in the kinase mode and a more rigid conformation in the phosphatase mode in response to signal perception by the periplasmic domains. We further show that the activity of BvgS is controlled in the same manner if its PAS domain is replaced with the natural α-helical sequences of PAS-less homologs. Clamshell motions of the Venus flytrap domains trigger the shift of the coiled coil’s dynamics. Thus, we have uncovered a general mechanism of regulation for the BvgS family of Venus flytrap-containing two-component sensor kinases. PMID:26933056

  11. Essential role of coiled-coils for aggregation and activity of Q/N-rich prions and polyQ proteins

    PubMed Central

    Fiumara, Ferdinando; Fioriti, Luana

    2012-01-01

    SUMMARY The functional switch of glutamine/asparagine (Q/N)-rich prions and the neurotoxicity of polyQ-expanded proteins involve complex aggregation-prone structural transitions, commonly presumed to be forming β-sheets. By analyzing sequences of interaction partners of these proteins, we discovered a recurrent presence of coiled-coil domains both in the partners and in segments that flank or overlap Q/N-rich and polyQ domains. Since coiled-coils can mediate protein interactions and multimerization, we studied their possible involvement in Q/N-rich and polyQ aggregations. Using circular dichroism and chemical cross-linking, we found that Q/N-rich and polyQ peptides form α-helical coiled-coils in vitro and assemble into multimers. Using structure-guided mutagenesis, we found that coiled-coil domains modulate in vivo properties of two Q/N-rich prions and polyQ-expanded huntingtin. Mutations that disrupt coiled-coils impair aggregation and activity, whereas mutations that enhance coiled-coil propensity promote aggregation. These findings support a coiled-coil model for the functional switch of Q/N-rich prions and for the pathogenesis of polyQ-expansion diseases. PMID:21183075

  12. Design and characterization of the anion-sensitive coiled-coil peptide.

    PubMed Central

    Hoshino, M.; Yumoto, N.; Yoshikawa, S.; Goto, Y.

    1997-01-01

    As a model for analyzing the role of charge repulsion in proteins and its shielding by the solvent, we designed a peptide of 27 amino acid residues that formed a homodimeric coiled-coil. The interface between the coils consisted of hydrophobic Leu and Val residues, and 10 Lys residues per monomer were incorporated into the positions exposed to solvent. During the preparation of a disulfide-linked dimer in which the two peptides were linked in parallel by the two disulfide bonds located at the N and C terminals, a cyclic monomer with an intramolecular disulfide bond was also obtained. On the basis of CD and 1H-NMR, the conformational stabilities of these isomers and several reference peptides were examined. Whereas all these peptides were unfolded in the absence of salt at pH 4.7 and 20 degrees C, the addition of NaClO4 cooperatively stabilized the alpha-helical conformation. The crosslinking of the peptides by disulfide bonds significantly decreased the midpoint salt concentration of the transition. The 1H-NMR spectra in the presence of NaClO4 suggested that, whereas the disulfide-bonded dimer assumed a native-like conformation, the cyclic monomer assumed a molten globule-like conformation with disordered side chains. However, the cyclic monomer exhibited cooperative transitions against temperature and Gdn-HCl that were only slightly less cooperative than those of the disulfide-bonded parallel dimer. These results indicate that the charge repulsion critically destabilizes the native-like state as well as the molten globule-like state, and that the solvent-dependent charge repulsion may be useful for controlling the conformation of designed peptides. PMID:9232640

  13. [Structure parameter selection of exiting coil in magnetic induction tomography].

    PubMed

    Ke, Li; Ling, Zhi; Du, Qiang; Hei, Jichang

    2013-02-01

    Magnetic induction tomography (MIT) is a kind of novel imaging method. In this system, exciting coil directly affects the detection range and detection sensitivity. In order to improve eddy current inductive region magnetic field distribution, we established a 3D four layers homocentric sphere head model in this study. Then we carried out a 3D three dimensional transient finite element calculation in different coil radius, turns and line diameter of the exciting coil of MIT system. Then we analyzed the axial magnetic flux density of the eddy current inductive area and detected the coil induced voltage. The research results showed that the increased magnetic flux density, the augmented detection range and the improved detection sensitivity were all realized by enlarging radius, increasing line diameter and reducing turns. PMID:23488128

  14. A Novel Coil Distribution for Transverse Flux Induction Heating

    NASA Astrophysics Data System (ADS)

    Sun, Yu; Wang, Youhua; Yang, Xiaoguang; Pang, Lingling

    For solving the problem of inhomogeneous temperature distribution on the surface of the work piece at the transverse flux induction heating (TFIH) device outlet, a novel coil distribution of the inductor is presented in this paper. The relationship between coil geometry and temperature distribution was analyzed firstly. According to the theoretical analysis results, the novel coil geometry was designed in order to get a uniform temperature distribution. Then the non-linear coupled electromagnetic- thermal problem in TFIH was simulated. The distributions of the magnetic flux density and eddy current of the novel and the traditional rectangular coil geometry were presented. Finally, a prototype was developed according to the numerical results. The experimental results of the temperature distribution agreed with the numerical analysis.

  15. Rapid mixing of viscous liquids by electrical coiling.

    PubMed

    Kong, Tiantian; Li, Jingmei; Liu, Zhou; Zhou, Zhuolong; Ng, Peter Hon Yu; Wang, Liqiu; Shum, Ho Cheung

    2016-01-01

    The control for the processing of precursor liquids determines whether the properties and functions of the final material product can be engineered. An inherent challenge of processing viscous liquids arises from their large resistance to deform. Here, we report on the discovery of an electric approach that can significantly contribute to address this challenge. The applied electric force can induce a straight viscous jet to coil, and the resulting coiling characteristics are governed by the electric stress. We demonstrate the promising use of electrical coiling in the rapid and efficient mixing of viscous liquids. Remarkably, the degree of mixing can be precisely adjusted by tuning the applied electric stress. Our approach of controlling the coiling electrically has important implications on applications such as dispensing and printing of resins, printing patterned surfaces and scaffolds, processing of food and generating non-woven fabrics. PMID:26860660

  16. Evaluation of plasma-based transmit coils for magnetic resonance.

    PubMed

    Webb, A G; Aussenhofer, S A

    2015-12-01

    In this work a new concept for designing transmit coils for magnetic resonance using a plasma is introduced. Unlike conventional coils, a plasma can be turned on and off, eliminating electrical interactions between coils, and enabling radiofrequency-invisibility when desired. A surfatron has been designed to produce a surface-mode wave which propagates along the inner surface of a commercial fluorescent lighting tube. NMR spectra and images have been produced using the plasma as the transmit coil and a copper-based monopole to receive the signal. The transmit efficiency of the plasma tube was estimated, and is currently much lower than that of an equivalently-sized metal-based structure: however, there are many potential methods for increasing the efficiency using a custom-built plasma tube. PMID:26529202

  17. Techniques For Microfabricating Coils For Microelectromechanical Systems Applications

    SciTech Connect

    Woods, R. C.; Powell, A. L.

    2008-01-21

    The advanced technology necessary for building future space exploration vehicles includes microfabricated coils for making possible self-inductances integrated with other passive and active electronic components. Integrated inductances make possible significant improvements in reliability over the traditional arrangement of using external discrete inductances, as well as allowing significant size (volume) reductions (also important in space vehicles). Two possible fabrication techniques (one using proprietary branded 'Foturan' glass, the other using silicon wafer substrates) for microscopic coils are proposed, using electroplating into channels. The techniques have been evaluated for fabricating the planar electrical coils needed for typical microelectromechanical systems applications. There remain problems associated with processing using 'Foturan' glass, but coil fabrication on silicon wafers was successful. Fabrication methods such as these are expected to play an important part in the development of systems and subsystems for forthcoming space exploration missions.

  18. Test data from the US-Demonstration Poloidal Coil experiment

    SciTech Connect

    Painter, T.A.; Steeves, M.M.; Takayasu, M.; Gung, C.; Hoenig, M.O. . Plasma Fusion Center); Tsuji, H.; Ando, T.; Hiyama, T.; Takahashi, Y.; Nishi, M.; Yoshida, K.; Okuno, K.; Nakajima, H.; Kato, T.; Sugimoto, M.; Isono, T.; Kawano, K.; Koizumi, N.; Osikiri, M.; Hanawa, H.; Ouchi, H.; Ono, M.; Ishida, H.; Hiue, H.; Yoshida, J.; Kamiyauchi, Y.; Ouchi, T.; Tajiri, F.

    1992-01-01

    The US Demonstration Poloidal Field Coil (US-DPC) experiment took place successfully at the Japan Atomic Energy Research Institute (JAERI) in late 1990. The 8 MJ niobium-tin coil was leak tight; it performed very well in DC tests; it performed well in AC tests, achieving approximately 70% of its design goal. An unexpected ramp-rate barrier at high currents was identified. The barrier could not be explored in the regime of higher fields and slower ramp rates due to limitations of the background-field coils. This document presents the results of the experiment with as little editing as possible. The coil, conductor, and operating conditions are given. The intent is to present data in a form that can be used by magnet analysts and designers.

  19. The toroidal field coil design for ARIES-ST

    SciTech Connect

    Reiersen, W.; Dahlgren, F.; Fan, H.M.; Neumeyer, C.; Zatz, I.

    2000-01-21

    An evolutionary process was used to develop the toroidal field (TF) coil design for the ARIES-ST (Spherical Tokamak). Design considerations included fabricability, assembly, maintenance, energy efficiency, and structural robustness. The design addresses a number of the concerns (complexity) and criticisms (high cost, high recirculating power) of fusion. It does this by: (1) Applying advanced, but available laser forming and spray casting techniques for manufacturing the TF coil system; (2) Adopting a simple single toroidal field coil system to make assembly and maintenance much easier, the single turn design avoids the necessity of using the insulation as a structural component of the TF coils, and hence is much more robust than multi-turn designs; and (3) Using a high conductivity copper alloy and modest current densities to keep the recirculating power modest.

  20. Rapid mixing of viscous liquids by electrical coiling

    PubMed Central

    Kong, Tiantian; Li, Jingmei; Liu, Zhou; Zhou, Zhuolong; Ng, Peter Hon Yu; Wang, Liqiu; Shum, Ho Cheung

    2016-01-01

    The control for the processing of precursor liquids determines whether the properties and functions of the final material product can be engineered. An inherent challenge of processing viscous liquids arises from their large resistance to deform. Here, we report on the discovery of an electric approach that can significantly contribute to address this challenge. The applied electric force can induce a straight viscous jet to coil, and the resulting coiling characteristics are governed by the electric stress. We demonstrate the promising use of electrical coiling in the rapid and efficient mixing of viscous liquids. Remarkably, the degree of mixing can be precisely adjusted by tuning the applied electric stress. Our approach of controlling the coiling electrically has important implications on applications such as dispensing and printing of resins, printing patterned surfaces and scaffolds, processing of food and generating non-woven fabrics. PMID:26860660

  1. MR imaging with remote reception using a coil array

    NASA Astrophysics Data System (ADS)

    Vazquez, F.; Marrufo, O.; Martin, R.; Rodriguez, A. O.

    2012-10-01

    A strategy for imaging a large field-of-view has recently been proposed applying remote detection with a waveguide and single loop coils. RF coils produce a traveling-wave propagating through the bore of the magnet, which is large enough so the cutoff frequency is below the Larmor frequency. This assumption also considers that a human subject inside the magnet bore. We applied the travelling-wave concept to generate images of a human leg at 3 Tesla. Two circular-shaped coils were used as the reception device and a whole-body coil was used for transmission. Images showed a good signal-to-noise ratio along the entire leg. This experimental results contradict the assumption that a whole-body 7T/65cm imager or higher was necessary to generate images with this approach.

  2. Measuring the off axis magnetic field within a Helmholtz Coil

    NASA Astrophysics Data System (ADS)

    Pluhar, Edward; Martell, Eric

    2013-03-01

    Helmholtz coils are used because they produce nearly uniform magnetic fields on-axis. Prior research, namely Graf's thin coil experiment [The Physics Teacher, pp. 360 (2012)], has accurately measured the axial magnetic field produced by a thin coil; however, the magnetic field off-axis is known to be significantly more complicated and cannot be calculated analytically. In this research, I have numerically determined the magnetic field off-axis in the region between the two coils and compared those calculations with measured values. I then determined the effect the deviation from uniformity has on the behavior of a charged particle moving through this region, such as in the well-known electron charge-to-mass ratio experiment.

  3. Application of NASTRAN to TFTR toroidal field coil structures

    NASA Technical Reports Server (NTRS)

    Chen, S. J.; Lee, E.

    1978-01-01

    The primary applied loads on the TF coils were electromagnetic and thermal. The complex structure and the tremendous applied loads necessitated computer type of solutions for the design problems. In the early stage of the TF coil design, many simplified finite element models were developed for the purpose of investigating the effects of material properties, supporting schemes, and coil case material on the stress levels in the case and in the copper coil. In the more sophisticated models that followed the parametric and scoping studies, the isoparametric elements, such as QUAD4, HEX8, and HEXA, were used. The analysis results from using these finite element models and the NASTRAN system were considered accurate enough to provide timely design information.

  4. Application of superconducting coils to the NASA prototype magnetic balance

    NASA Technical Reports Server (NTRS)

    Haldeman, C. W.; Kraemer, R. A.; Phey, S. W.; Alishahi, M. M.; Covert, E. E.

    1981-01-01

    Application of superconducting coils to a general purpose magnetic balance was studied. The most suitable currently available superconducting cable for coils appears to be a bundle of many fine wires which are transposed and are mechanically confined. Sample coils were tested at central fields up to .5 Tesla, slewing rates up to 53 Tesla/ sec and frequencies up to 30 Hz. The ac losses were measured from helium boil-off and were approximately 20% higher than those calculated. Losses were dominated by hysteresis and a model for loss calculation which appears suitable for design purposes is presented along with computer listings. Combinations of two coils were also tested and interaction losses are reported. Two feasible geometries are also presented for prototype magnetic balance using superconductors.

  5. 1. PLENUM INTERIOR, SHOWING HEATING COILS AND BYPASS Hot ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. PLENUM INTERIOR, SHOWING HEATING COILS AND BY-PASS - Hot Springs National Park, Bathhouse Row, Fordyce Bathhouse: Mechanical & Piping Systems, State Highway 7, 1 mile north of U.S. Highway 70, Hot Springs, Garland County, AR

  6. Bi-2223 HTS winding in toroidal configuration for SMES coil

    NASA Astrophysics Data System (ADS)

    Kondratowicz-Kucewicz, B.; Janowski, T.; Kozak, S.; Kozak, J.; Wojtasiewicz, G.; Majka, M.

    2010-06-01

    Energy can be stored in the magnetic field of a coil. Superconducting Magnetic Energy Storage (SMES) is very promising as a power storage system for load levelling or power stabilizer. However, the strong electromagnetic force caused by high magnetic field and large coil current is a problem in SMES systems. A toroidal configuration would have a much less extensive external magnetic field and electromagnetic forces in winding. The paper describes the design of HTS winding for SMES coil in modular toroid configuration consist of seven Bi-2223 double-pancakes as well as numerical analysis of SMES magnet model using FLUX 3D package. As the results of analysis the paper presents the optimal coil configuration and the parameters such as radius of toroidal magnet, energy stored in magnet and magnetic field distribution.

  7. Rapid mixing of viscous liquids by electrical coiling

    NASA Astrophysics Data System (ADS)

    Kong, Tiantian; Li, Jingmei; Liu, Zhou; Zhou, Zhuolong; Ng, Peter Hon Yu; Wang, Liqiu; Shum, Ho Cheung

    2016-02-01

    The control for the processing of precursor liquids determines whether the properties and functions of the final material product can be engineered. An inherent challenge of processing viscous liquids arises from their large resistance to deform. Here, we report on the discovery of an electric approach that can significantly contribute to address this challenge. The applied electric force can induce a straight viscous jet to coil, and the resulting coiling characteristics are governed by the electric stress. We demonstrate the promising use of electrical coiling in the rapid and efficient mixing of viscous liquids. Remarkably, the degree of mixing can be precisely adjusted by tuning the applied electric stress. Our approach of controlling the coiling electrically has important implications on applications such as dispensing and printing of resins, printing patterned surfaces and scaffolds, processing of food and generating non-woven fabrics.

  8. Critical Current Measurements in Commercial Tapes, Coils, and Magnets.

    NASA Astrophysics Data System (ADS)

    Gubser, D. U.; Soulen, R. J., Jr.; Fuller-Mora, W. W.; Francavilla, T. L.

    1996-03-01

    We have measured a number of tapes, coils, and magnets produced by commercial vendors and determined their properties as functions of magnetic field and temperature. The tapes were measured at the National High Magnetic Field Laboratory in magnetic fields to 20 tesla and at temperatures of 4.2 K, 27 K, 65 K, and 77 K. For the tapes we report critical currents and current-voltage characteristics. Six inch diameter coils were measured at NRL in zero magnetic field. Critical currents, current-voltage characteristics, and reliability studies are reported for the coils. Larger 10 inch diameter coils, which are to be used in a 200 hp superconducting motor, were also measured and results will be presented. The talk will also review the status of the most recent tests of the superconducting motor.

  9. Convective heat transfer enhancement inside tubes using inserted helical coils

    NASA Astrophysics Data System (ADS)

    Ali, R. K.; Sharafeldeen, M. A.; Berbish, N. S.; Moawed, M. A.

    2016-01-01

    Convective heat transfer was experimentally investigated in tubes with helical coils inserts in turbulent flow regime within Reynolds number range of 14400 ≤ Re ≤ 42900. The present work aims to extend the experimental data available on wire coil inserts to cover wire diameter ratio from 0.044 to 0.133 and coil pitch ratio from 1 to 5. Uniform heat flux was applied to the external surface of the tube and air was selected as fluid. The effects of Reynolds number and wire diameter and coil pitch ratios on the Nusselt number and friction factor were studied. The enhancement efficiency and performance criteria ranges are of (46.9-82.6%) and (100.1-128%) within the investigated range of the different parameters, respectively. Correlations are obtained for the average Nusselt number and friction factor utilizing the present measurements within the investigated range of geometrical parameters and Re.

  10. High-sensitivity broadband microwave spectroscopy with small nonresonant coils

    NASA Astrophysics Data System (ADS)

    Mahdjour, H.; Clark, W. G.; Baberschke, K.

    1986-06-01

    The use of a small, nonresonant, coil of micron dimensions as the microwave magnetic field structure of a broadband electron-spin-resonance (ESR) spectrometer is described. The coil is driven by a broadband microwave generator which operates between 0.1 and 8.5 GHz. The samples may fill the coil to approximately 100 percent. It is shown that for small size samples this system offers higher sensitivity than a conventional cavity spectrometer. Because the system is broadband, either frequency scans or the conventional magnetic field scans can be used to traverse the resonance. Examples for DPPH and for the spin glass AgMn using this method are reported. Since the sample coil is small, it has many potential applications, such as insertion into the mixing chamber of dilution refrigerator or high-pressure cell, efficient use of power in ENDOR and other double resonance experiments, and rapid recovery from transients in pulsed ESR experiments.

  11. Coil combination of multichannel MRSI data at 7 T: MUSICAL.

    PubMed

    Strasser, B; Chmelik, M; Robinson, S D; Hangel, G; Gruber, S; Trattnig, S; Bogner, W

    2013-12-01

    The goal of this study was to evaluate a new method of combining multi-channel (1)H MRSI data by direct use of a matching imaging scan as a reference, rather than computing sensitivity maps. Seven healthy volunteers were measured on a 7-T MR scanner using a head coil with a 32-channel array coil for receive-only and a volume coil for receive/transmit. The accuracy of prediction of the phase of the (1)H MRSI data with a fast imaging pre-scan was investigated with the volume coil. The array coil (1)H MRSI data were combined using matching imaging data as coil combination weights. The signal-to-noise ratio (SNR), spectral quality, metabolic map quality and Cramér-Rao lower bounds were then compared with the data obtained by two standard methods, i.e. using sensitivity maps and the first free induction decay (FID) data point. Additional noise decorrelation was performed to further optimize the SNR gain. The new combination method improved significantly the SNR (+29%), overall spectral quality and visual appearance of metabolic maps, and lowered the Cramér-Rao lower bounds (-34%), compared with the combination method based on the first FID data point. The results were similar to those obtained by the combination method using sensitivity maps, but the new method increased the SNR slightly (+1.7%), decreased the algorithm complexity, required no reference coil and pre-phased all spectra correctly prior to spectral processing. Noise decorrelation further increased the SNR by 13%. The proposed method is a fast, robust and simple way to improve the coil combination in (1)H MRSI of the human brain at 7 T, and could be extended to other (1)H MRSI techniques. PMID:24038331

  12. Development and testing of the cooling coil cleaning end effector

    SciTech Connect

    Johnson, K.I.; Mullen, O.D.; Powell, M.R.; Daly, D.S.; Engel, D.W.

    1997-09-30

    The Retrieval Process Development and Enhancement (KPD{ampersand}E) program has developed and tested an end effector to support the waste retrieval mission at the Idaho National Engineering and Environmental Laboratory (INEEL). The end effector was developed specifically to remove a sticky waste material from the cooling coils in the High Level Liquid Waste (HLLW) tank, and to vacuum up a sediment layer that has settled beneath the cooling coils. An extensive testing program was conducted in the hydraulic test bed (HTB) at the Pacific Northwest National Laboratory (PNNL) to evaluate the performance of the end effector under simulated in-tank conditions. A mock up of the cooling coils was installed in the test bed tank, and simulated waste materials were included to represent the sticky waste on the tubes and the particulate waste settled beneath them. The testing program focused on assessing long-duration mining strategies for cleaning the cooling coils and removing the particulate waste forms. The report describes the results of the end effector testing program at PNNL. Section 2 describes the physical characteristics of the HLLW tanks, including the layout of the cooling coils, and it also describes what is known of the waste forms in the tanks. Section 3 describes the cleaning and retrieval strategy that was used in developing the end effector design. Section 4 describes the cooling coil mockup in the hydraulic test bed. Section 5 discusses the rationale used in selecting the simulants for the tarry waste and particulate waste forms. Section 6 describes the tests that were performed to evaluate cleaning of the cooling coils and retrieval of the particulate simulant. Section 7 summarizes the cleaning and retrieval tests, assesses the relative importance of cleaning the cooling coils and retrieving the particulate waste, and suggests modifications that would simplify the end effector design.

  13. NSTX-U Digital Coil Protection System Software Detailed Design

    SciTech Connect

    2014-06-01

    The National Spherical Torus Experiment (NSTX) currently uses a collection of analog signal processing solutions for coil protection. Part of the NSTX Upgrade (NSTX-U) entails replacing these analog systems with a software solution running on a conventional computing platform. The new Digital Coil Protection System (DCPS) will replace the old systems entirely, while also providing an extensible framework that allows adding new functionality as desired.

  14. Temporal Behaviour of ECR Plasmas Produced by a Lisitano Coil

    NASA Astrophysics Data System (ADS)

    Suetsugu, Yusuke; Kawai, Yoshinobu

    1984-02-01

    In order to clarify the mechanism of production of a plasma produced by electron cyclotron resonance heating (ECRH) using a Lisitano coil, the temporal behaviour of the plasma was measured in detail by a sampling method. It was found that the plasma is produced not only around the Lisitano coil but also near the center. The plasma density was also confirmed to attain a value above the cutoff density by measuring the field intensities of the microwaves.

  15. A Promising Adjuvant to Detachable Coils for Cavernous Packing: Onyx

    PubMed Central

    Lv, X.; Jiang, C.; Li, Y.; Wu, Z.

    2009-01-01

    Summary Transvenous embolization of cavernous dural arteriovenous fistulae (CDAVFs) with Onyx has recently been reported. This study was undertaken to assess the value of Onyx in transvenous treatment of CDAVFs. We retrospectively reviewed 18 patients who underwent transvenous embolization for CDAVFs of Barrow Type D with detachable coils and Onyx at our institution over five years. Patients were divided into two groups: group A, patients who had been treated with detachable coils; group B, patients who had been treated with a combination of detachable coils and Onyx. The approach routes, angiographic results, complications and clinical outcome were assessed for both groups. Eighteen patients with CDAVFs of Barrow Type D were treated: nine women and nine men; mean age was 41.9 years. Eleven patients treated by 19 procedures of transvenous coiling belonged to group A. Seven patients treated by eight procedures of transvenous Onyx injection belonged to group B. The periprocedural complication rate associated with coiling for both groups was 18.2% vs 16.7% with Onyx. The duration of the procedure in both groups was 6.77±2.49 hours vs 3.75±1.63 hours with coiling vs Onyx, and the cost of Onyx was cheaper than coils. An excellent outcome was achieved in both groups: 90.9% vs 100% (group A vs group B). Our results associated with both modalities of CDAVFs treatment with clinical outcome show that transvenous embolization with Onyx is a safe alternative to detachable coils in the treatment of CDAVFs. However, more cases need to be evaluated. PMID:20465891

  16. Real-Time Coil Position Monitoring System for Biomagnetic Measurements

    NASA Astrophysics Data System (ADS)

    Oyama, Daisuke; Adachi, Yoshiaki; Higuchi, Masanori; Kawai, Jun; Kobayashi, Koichiro; Uehara, Gen

    In this paper, we propose a new method for monitoring the position of marker coils. The marker coil is used for indicating spatial relationship between subject's body and magnetic sensor arrays in biomagnetic measurements, such as magnetoencephalography (MEG) and magnetocardiography (MCG). We developed a real-time marker coil position monitoring system combined with a conventional MEG system. In order to achieve simultaneous measurement of MEG signals and marker signals, we separated their frequency bands. The frequency bands of flux-locked loop (FLL) circuits were separated into three parts by three integrators; low-band, mid-band, and high-band. The second and third bands were assigned for MEG signals and marker signals, respectively. This method can avoid the crosstalk of the marker signals to MEG signals. Marker signals were generated from five marker coils driven by five independent sinusoidal current generators. These signals were continuously measured by the high-band of FLL, and then the coils were localized by FFT processing and solving inverse problem. We succeeded in displaying the localized coil position on a PC monitor once per second in real-time.

  17. Fabrication of Spiral Micro Coil Lines for Electromagnetic Actuators

    NASA Astrophysics Data System (ADS)

    Setomoto, Masaru; Matsumoto, Yoshifumi; Yamashita, Shuhei; Noda, Daiji; Hattori, Tadashi

    With the recent progress in downsizing and the sophistication of various industrial products, the need for more compact actuators is increasing. Actuators account for the larger percentage of volume and weight of a product compared with other parts and devices. We have proposed fabrication process of spiral micro coils that employs X-ray lithography. This process will be effective for fabricating coils of a high aspect ratio lines. Reducing the size of coil lines and increasing their aspect ratio are expected to reduce the size and increase the output of actuators. Using this process, we formed spiral coil lines that can be used in electromagnetic actuators. X-ray lithography was used to form a high aspect ratio helical structure on the surface of an acrylic resin pipe. As a measure to suppress void generation, which is one of the shortcomings of electroplating processes, the sputtering apparatus and plating equipment were improved, a pretreatment process was additionally provided, and the actual electroplating method was improved. As a result, a void-free metallic deposit could be formed on a thin coil line. At the final step of this research study, we etched the coil line to determine optimal etching conditions.

  18. High-current density coils for high-radiation environments

    SciTech Connect

    Harvey, A.

    1981-01-01

    This paper concentrates on the problems of providing normal (that is, nonsuperconducting) magnet coils for present and short-term-future requirements where significant radiation doses are involved. Projects such as 100-mA deuteron accelerators and bundle diverter coils for TOKAMAKS are typical of applications where conventional organic insulation limited to 10/sup 10/ rads makes epoxy-based systems unacceptable. Moreover, even in present-day accelerators, radiation levels can be high enough to give rise to problems with oxidation of copper conductors if water is used in direct contact with the copper. The radiolytic oxygen, being formed in situ, cannot be controlled by external deoxygenators. An acceptable insulation for such environments has been described previously, and is being employed where radiation is expected to be a problem. Being a compacted magnesium oxide powder, the insulation has advantages. Analysis of constraints on maximum current densities achievable in such a coil construction, using computer codes, leads to coil configurations that operate at higher current densities than are usually found in directly cooled coils. An example of the thermal analysis of one coil configuration is given. The problems are addressed here.

  19. Automatic coil selection for streak artifact reduction in radial MRI.

    PubMed

    Xue, Yiqun; Yu, Jiangsheng; Kang, Hyun Seon; Englander, Sarah; Rosen, Mark A; Song, Hee Kwon

    2012-02-01

    In radial MR imaging, streaking artifacts contaminating the entire field of view can arise from regions at the outer edges of the prescribed field of view. This can occur even when the Nyquist criterion is satisfied within the desired field of view. These artifacts become exacerbated when parts of the object lie in the superior/inferior regions of the scanner where the gradient strengths become weakened. When multiple coil arrays are used for signal reception, coils at the outer edges can be disabled before data acquisition to reduce the artifact levels. However, as the weakened gradient strengths near the edges often distort the object, causing the signal to become highly concentrated into a small region, the streaks are often not completely removed. Data from certain coils can also be excluded during reconstruction by visually inspecting the individual coil images, but this is impractical for routine use. In this work, a postprocessing method is proposed to automatically identify those coils whose images contain high levels of streaking for subsequent exclusion during reconstruction. The proposed method was demonstrated in vivo dynamic contrast enhanced MRI datasets acquired using a three-dimensional hybrid radial sequence. The results demonstrate that the proposed strategy substantially improves the image quality and show excellent agreement with images reconstructed with manually determined coil selection. PMID:21656562

  20. Dynamic multi-channel TMS with reconfigurable coil.

    PubMed

    Jiang, Ruoli; Jansen, Ben H; Sheth, Bhavin R; Chen, Ji

    2013-05-01

    Investigations of the causal involvement of particular brain areas and interconnections in behavior require an external stimulation system with reasonable spatio-temporal resolution. Current transcranial magnetic stimulation (TMS) technology is limited to stimulating a single brain area once in a given trial. Here, we present a feasibility study for a novel TMS system based on multi-channel reconfigurable coils. With this hardware, researchers will be able to stimulate multiple brain sites in any temporal order in a trial. The system employs a wire-mesh coil, constructed using x- and y-directional wires. By varying the current direction and/or strength on each wire, we can configure the proposed mesh-wire coil into a standard loop coil and figure-eight coil of varying size. This provides maximum flexibility to the experimenter in that the location and extent of stimulation on the brain surface can be modified depending on experimental requirement. Moreover, one can dynamically and automatically modify the site(s) of stimulation several times within the span of seconds. By pre-storing various sequences of excitation patterns inside a control unit, one can explore the effect of dynamic TMS on behavior, in associative learning, and as rehabilitative therapy. Here, we present a computer simulation and bench experiments that show the feasibility of the dynamically-reconfigurable coil. PMID:23193321

  1. NMR of thin layers using a meanderline surface coil

    DOEpatents

    Cowgill, Donald F.

    2001-01-01

    A miniature meanderline sensor coil which extends the capabilities of nuclear magnetic resonance (NMR) to provide analysis of thin planar samples and surface layer geometries. The sensor coil allows standard NMR techniques to be used to examine thin planar (or curved) layers, extending NMRs utility to many problems of modern interest. This technique can be used to examine contact layers, non-destructively depth profile into films, or image multiple layers in a 3-dimensional sense. It lends itself to high resolution NMR techniques of magic angle spinning and thus can be used to examine the bonding and electronic structure in layered materials or to observe the chemistry associated with aging coatings. Coupling this sensor coil technology with an arrangement of small magnets will produce a penetrator probe for remote in-situ chemical analysis of groundwater or contaminant sediments. Alternatively, the sensor coil can be further miniaturized to provide sub-micron depth resolution within thin films or to orthoscopically examine living tissue. This thin-layer NMR technique using a stationary meanderline coil in a series-resonant circuit has been demonstrated and it has been determined that the flat meanderline geometry has about he same detection sensitivity as a solenoidal coil, but is specifically tailored to examine planar material layers, while avoiding signals from the bulk.

  2. Thermal Stability Analysis for Superconducting Coupling Coil in MICE

    SciTech Connect

    Wu, Hong; Wang, Li; Pan, Heng; Guo, XingLong; Green, M.A.

    2010-06-28

    The superconducting coupling coil to be used in the Muon Ionization Cooling Experiment (MICE) with inner radius of 750 mm, length of 285 mm and thickness of 110.4 mm will be cooled by a pair of 1.5 W at 4.2 K cryo-coolers. When the coupling coil is powered to 210 A, it will produce about 7.3 T peak magnetic field at the conductor and it will have a stored energy of 13 MJ. A key issue for safe operation of the coupling coil is the thermal stability of the coil during a charge and discharge. The magnet and its cooling system are designed for a rapid discharge where the magnet is to be discharged in 5400 seconds. The numerical simulation for the thermal stability of the MICE coupling coil has been done using ANSYS. The analysis results show that the superconducting coupling coil has a good stability and can be charged and discharged safely.

  3. RF Coil Considerations for Short-T2 MRI

    PubMed Central

    Horch, R. Adam; Wilkens, Ken; Gochberg, Daniel F.; Does, Mark D.

    2010-01-01

    With continuing hardware and pulse sequence advancements, modern MRI is gaining sensitivity to signals from short-T2 1H species under practical experimental conditions. However, conventional MRI coils are typically not designed for this type of application they often contain proton-rich construction materials which may contribute confounding 1H background signal during short-T2 measurements. An example of this is shown herein. Separately, a loop-gap style coil was used to compare different coil construction materials and configurations with respect to observed 1H background signal sizes in a small animal imaging system. Background signal sources were spatially identified and quantified in a number of different coil configurations. It was found that the type and placement of structural coil materials around the loop-gap resonator, as well as the coil’s shielding configuration, are critical determinants of the coil’s background signal size. Although this study employed a loop-gap resonator design, these findings are directly relevant to standard volume coils commonly used for MRI. PMID:20665825

  4. Performance of a 12-coil superconducting bumpy torus magnet facility

    NASA Technical Reports Server (NTRS)

    Roth, J. R.; Holmes, A. D.; Keller, T. A.; Krawczonek, W. M.

    1972-01-01

    The bumpy torus facility consists of 12 superconducting coils, each 19 cm i.d. and capable of 3.0 teslas on their axes. The coils are equally spaced around a toroidal array with a major diameter of 1.52 m, and are mounted with the major axis of the torus vertical in a single vacuum tank 2.6 m in diameter. Final shakedown tests of the facility mapped out its magnetic, cryogenic, vacuum, mechanical, and electrical performance. The facility is now ready for use as a plasma physics research facility. A maximum magnetic field on the magnetic axis of 3.23 teslas was held for a period of more than sixty minutes without a coil normalcy. The design field was 3.00 teslas. The steady-state liquid helium boil-off rate was 87 liters per hour of liquid helium without the coils charged. The coil array was stable when subjected to an impulsive loading, even with the magnets fully charged. When the coils were charged to a maximum magnetic field of 3.35 teslas, the system was driven normal without damage.

  5. Release of zinc from disposable coils during hemodialysis

    SciTech Connect

    Bogden, J.D.; Zadzielski, E.; Weiner, B.; Oleske, J.M.; Aviv, A.

    1981-06-01

    In a prior study, we demonstrated that certain disposable coils are contaminated with zinc and release substantial quantities of Zn during hemodialysis, producing high post-dialysis plasma Zn concentrations. The present investigation was designed to monitor plasma and dialysis fluid Zn and copper throughout dialysis and to estimate patient Zn and Cu uptake. Venous plasma, arterial plasma and coil chamber fluid were sampled periodically throughout dialysis; trace metal concentrations were determined by flame atomic absorption spectrophotometry. Release of considerable quantities of Zn from the coils into the dialysis fluid, with uptake into the patient's plasma, was found. Approximately one-half of the plasma Zn uptake occurred in the first 45 minutes. Coils from different lots released significantly different quantities of Zn. Patient uptake of Zn ranged from 3.2 mg-23.0 mg, with a mean of 15.0 +- 6.1 mg. Copper release and uptake was low. It is recommended that quality control of the Zn content of some types of disposable coils be initiated. The results suggest that Zn release from disposable dialysis coils should be assessed before recommending that hemodialysis patients receive Zn supplements.

  6. Quenching characteristics of a.c. superconducting coils

    NASA Astrophysics Data System (ADS)

    Akita, S.; Kasahara, H.; Torii, S.

    Quenching characteristics of two superconducting coils for a.c. use are investigated at different frequencies. One is impregnated with epoxy resin and the other is not. Both coils have experienced over 40 quenches in a.c. and d.c. The impregnated coil shows steady quenching currents for a.c. and the values are nearly the same as for d.c. On the other hand, quenching currents for a.c. in the non-impregnated coil are almost 80% of the trained-up d.c. quenching current and are scattered. Furthermore, the relationship between quenching currents and the estimated a.c. losses of the superconducting cable at the highest magnetic field point is investigated. According to the results of this investigation, the cause of quench in the impregnated coil is assumed to be the temperature rise of the winding due to a.c. losses, while the cause in the non-impregnated coil might be wire motion.

  7. Design of a superconducting 20 MJ induction heating coil

    SciTech Connect

    Singh, S.K.; Ibrahim, E.A.; Gaberson, P.C.; Eckels, P.W.; Jarabak, A.J.; Rogers, J.D.; Thullen, P.; Walker, M.S.

    1980-01-01

    A pancake-wound, low-loss, superconducting, induction-heating coil has been designed to demonstrate the feasibility of superconducting polaidal system for the Tokamak reactors, to provide confidence in application of superconductivity to actual reactors, and to provide the opportunity to solve specific engineering problems to support the fusion pulsed coil program. the coil is designed to store 20 MJ at 50 kA. The superconductor material is NbTi for a 7.5 tesla maximum field. The coil is designed to survive at least 100,000 cycles of full bipolar half cycle sinusoidal operation from +7.5 tesla to -7.5 telsa fields in one second. The coil is natural convection immersion-cooled at 4.5/sup 0/K in liquid helium bath. The design demonstrates confidence in an advanced design, low-loss, cryostable conductor, along with safety, reliability and the operating life of the coil of more than 100,000 cycles.

  8. The Effect of Axial Stress on YBCO Coils

    SciTech Connect

    Sampson, W.; Anerella, M.; Cozzolino, J.P.; Gupta, R.C.; Shiroyanagi, Y.; Evangelou, E.

    2011-03-28

    The large aspect ratio of typical YBCO conductors makes them ideal for constructing solenoids from pancake style coils. An advantage of this method is that each subunit can be tested before assembly into the finished magnet. The fact that conductors are available in relatively short lengths is another reason for using such a fabrication technique. The principal drawback is the large number of joints required to connect the coils together. When very high field solenoids such as those contemplated for the muon collider are built in this way the magnetic forces between pancakes can be very large. Extensive measurements have been made on the effect of stress on short lengths of conductor, but there is little or no data on the effect of intercoil loading. The experiment described in this paper was designed to test the ability of YBCO coils to withstand these forces. A spiral wound 'pancake' coil made from YBCO coated conductor has been stressed to a pressure of 100MPa in the axial direction at 77K. In this case axial refers to the coil so that the force is applied to the edge of the conductor. The effect on the critical current was small and completely reversible. Repeatedly cycling the pressure had no measureable permanent effect on the coil. The small current change observed exhibited a slight hysteretic behaviour during the loading cycle.

  9. A 3T Sodium and Proton Composite Array Breast Coil

    PubMed Central

    Kaggie, Joshua D.; Hadley, J. Rock; Badal, James; Campbell, John R.; Park, Daniel J.; Parker, Dennis L.; Morrell, Glen; Newbould, Rexford D.; Wood, Ali F.; Bangerter, Neal K.

    2013-01-01

    Purpose The objective of this study was to determine whether a sodium phased array would improve sodium breast MRI at 3T. The secondary objective was to create acceptable proton images with the sodium phased array in place. Methods A novel composite array for combined proton/sodium 3T breast MRI is compared to a coil with a single proton and sodium channel. The composite array consists of a 7-channel sodium receive array, a larger sodium transmit coil, and a 4-channel proton transceive array. The new composite array design utilizes smaller sodium receive loops than typically used in sodium imaging, uses novel decoupling methods between the receive loops and transmit loops, and uses a novel multi-channel proton transceive coil. The proton transceive coil reduces coupling between proton and sodium elements by intersecting the constituent loops to reduce their mutual inductance. The coil used for comparison consists of a concentric sodium and proton loop with passive decoupling traps. Results The composite array coil demonstrates a 2–5x improvement in SNR for sodium imaging and similar SNR for proton imaging when compared to a simple single-loop dual resonant design. Conclusion The improved SNR of the composite array gives breast sodium images of unprecedented quality in reasonable scan times. PMID:24105740

  10. Rapid Identification of Malaria Vaccine Candidates Based on α-Helical Coiled Coil Protein Motif

    PubMed Central

    Villard, Viviane; Agak, George W.; Frank, Géraldine; Jafarshad, Ali; Servis, Catherine; Nébié, Issa; Sirima, Sodiomon B.; Felger, Ingrid; Arevalo-Herrera, Myriam; Herrera, Socrates; Heitz, Frederic; Bäcker, Volker; Druilhe, Pierre; Kajava, Andrey V.; Corradin, Giampietro

    2007-01-01

    To identify malaria antigens for vaccine development, we selected α-helical coiled coil domains of proteins predicted to be present in the parasite erythrocytic stage. The corresponding synthetic peptides are expected to mimic structurally “native” epitopes. Indeed the 95 chemically synthesized peptides were all specifically recognized by human immune sera, though at various prevalence. Peptide specific antibodies were obtained both by affinity-purification from malaria immune sera and by immunization of mice. These antibodies did not show significant cross reactions, i.e., they were specific for the original peptide, reacted with native parasite proteins in infected erythrocytes and several were active in inhibiting in vitro parasite growth. Circular dichroism studies indicated that the selected peptides assumed partial or high α-helical content. Thus, we demonstrate that the bioinformatics/chemical synthesis approach described here can lead to the rapid identification of molecules which target biologically active antibodies, thus identifying suitable vaccine candidates. This strategy can be, in principle, extended to vaccine discovery in a wide range of other pathogens. PMID:17653272

  11. Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif

    PubMed Central

    Hirtreiter, Angela; Damsma, Gerke E.; Cheung, Alan C. M.; Klose, Daniel; Grohmann, Dina; Vojnic, Erika; Martin, Andrew C. R.; Cramer, Patrick; Werner, Finn

    2010-01-01

    Spt5 is the only known RNA polymerase-associated factor that is conserved in all three domains of life. We have solved the structure of the Methanococcus jannaschii Spt4/5 complex by X-ray crystallography, and characterized its function and interaction with the archaeal RNAP in a wholly recombinant in vitro transcription system. Archaeal Spt4 and Spt5 form a stable complex that associates with RNAP independently of the DNA–RNA scaffold of the elongation complex. The association of Spt4/5 with RNAP results in a stimulation of transcription processivity, both in the absence and the presence of the non-template strand. A domain deletion analysis reveals the molecular anatomy of Spt4/5—the Spt5 Nus-G N-terminal (NGN) domain is the effector domain of the complex that both mediates the interaction with RNAP and is essential for its elongation activity. Using a mutagenesis approach, we have identified a hydrophobic pocket on the Spt5 NGN domain as binding site for RNAP, and reciprocally the RNAP clamp coiled-coil motif as binding site for Spt4/5. PMID:20197319

  12. An engineered coiled-coil polypeptide assembled onto quantum dots for targeted cell imaging

    NASA Astrophysics Data System (ADS)

    Yao, Ming-Hao; Yang, Jie; Song, Ji-Tao; Zhang, Lin; Fang, Bi-Yun; Zhao, Dong-Hui; Xia, Rui-Xue; Jin, Rui-Mei; Zhao, Yuan-Di; Liu, Bo

    2015-12-01

    Quantum dot (QD)-polypeptide probes have been developed through the specific metal-affinity interaction between polypeptides appended with N-terminal polyhistidine sequences and CdSe/ZnS core-shell QDs. The size and charge of a QD-polypeptide can be tuned by using different coiled-coil polypeptides. Compared to glutathione-capped QDs (QD-GSH), QD-polypeptide probes showed an approximately two- to three-fold luminescence increase, and the luminescence increase was not obviously related to the charge of the polypeptide. QD-polypeptide probes with different charge have a great effect on nonspecific cellular uptake. QD-polypeptide probes with negative charge exhibited lower nonspecific cellular uptake in comparison to the QD-GSH, while positively charged QD-polypeptide probes presented higher cellular uptake than the QD-GSH. A targeted QD-ARGD probe can obviously increase targeted cellular uptake in α v β 3 overexpressing HeLa cells compared to QD-A. In addition, QD-polypeptide probes showed lower in vitro cytotoxicity compared to the original QDs. These results demonstrate that these QD-polypeptide probes with high specific cellular uptake, high fluorescence intensity and low background noise are expected to have great potential applications in targeted cell imaging.

  13. Structural domains of vault proteins: a role for the coiled coil domain in vault assembly.

    PubMed

    van Zon, Arend; Mossink, Marieke H; Schoester, Martijn; Scheffer, George L; Scheper, Rik J; Sonneveld, Pieter; Wiemer, Erik A C

    2002-03-01

    Vaults consist of multiple copies of three proteins (MVP, VPARP, and TEP1) and several untranslated RNAs. The function of vaults is unknown but the typical and evolutionary conserved structure indicates a role in intracellular transport. Although all vault components have been identified and characterized, not much is known about vault protein assembly. In this study we identified and analyzed structural domains involved in vault assembly with emphasis on protein-protein interactions. Using a yeast two-hybrid system, we demonstrate within MVP an intramolecular binding site and show that MVP molecules interact with each other via their coiled coil domain. We show that purified MVP is able to bind calcium, most likely at calcium-binding EF-hands. No interactions could be detected between TEP1 and other vault proteins. However, the N-terminal half of MVP binds to a specific domain in the C-terminus of VPARP. Furthermore, VPARP contains amino acid stretches mediating intramolecular binding. PMID:11855821

  14. Ndm, a coiled-coil domain protein that suppresses macropinocytosis and has effects on cell migration.

    PubMed

    Kelsey, Jessica S; Fastman, Nathan M; Noratel, Elizabeth F; Blumberg, Daphne D

    2012-09-01

    The ampA gene has a role in cell migration in Dictyostelium discoideum. Cells overexpressing AmpA show an increase in cell migration, forming large plaques on bacterial lawns. A second-site suppressor of this ampA-overexpressing phenotype identified a previously uncharacterized gene, ndm, which is described here. The Ndm protein is predicted to contain a coiled-coil BAR-like domain-a domain involved in endocytosis and membrane bending. ndm-knockout and Ndm-monomeric red fluorescent protein-expressing cell lines were used to establish a role for ndm in suppressing endocytosis. An increase in the rate of endocytosis and in the number of endosomes was detected in ndm(-) cells. During migration ndm(-) cells formed numerous endocytic cups instead of the broad lamellipodia structure characteristic of moving cells. A second lamellipodia-based function-cell spreading-was also defective in the ndm(-) cells. The increase in endocytosis and the defect in lamellipodia formation were associated with reduced chemotaxis in ndm(-) cells. Immunofluorescence results and glutathione S-transferase pull-down assays revealed an association of Ndm with coronin and F-actin. The results establish ndm as a gene important in regulating the balance between formation of endocytic cups and lamellipodia structures. PMID:22809629

  15. Tropomyosin is an interaction partner of the Drosophila coiled coil protein yuri gagarin.

    PubMed

    Texada, Michael J; Simonette, Rebecca A; Deery, William J; Beckingham, Kathleen M

    2011-02-15

    The Drosophila gene yuri gagarin is a complex locus encoding three protein isoform classes that are ubiquitously expressed in the organism. Mutations to the gene affect processes as diverse as gravitactic behavior and spermatogenesis. The larger Yuri isoforms contain extensive coiled-coil regions. Our previous studies indicate that one of the large isoform classes (Yuri-65) is required for formation of specialized F-actin-containing structures generated during spermatogenesis, including the so-called actin "cones" that mediate spermatid individualization. We used the tandem affinity purification of a tagged version of Yuri-65 (the TAP-tagging technique) to identify proteins associated with Yuri-65 in the intact organism. Tropomyosin, primarily as the 284-residue isoform derived from the ubiquitously expressed Tropomyosin 1 gene was thus identified as a major Yuri interaction partner. Co-immunoprecipitation experiments confirmed this interaction. We have established that the stable F-actin cones of spermatogenesis contain Tropomyosin 1 (Tm1) and that in mutant yuri(F64), failure of F-actin cone formation is associated with failure of Tm1 to accumulate at the cone initiation sites. In investigating possible interactions of Tm1 and Yuri in other tissues, we discovered that Tm1 and Yuri frequently colocalize with the endoplasmic reticulum. Tropomyosin has been implicated in actin-mediated membrane trafficking activity in other systems. Our findings suggest that Yuri-Tm1 complexes participate in related functions. PMID:21126519

  16. TROPOMYOSIN IS AN INTERACTION PARTNER OF THE DROSOPHILA COILED COIL PROTEIN YURI GAGARIN

    PubMed Central

    Texada, Michael J.; Simonette, Rebecca A.; Deery, William J.; Beckingham, Kathleen M.

    2011-01-01

    The Drosophila gene yuri gagarin is a complex locus encoding three protein isoform classes that are ubiquitously expressed in the organism. Mutations to the gene affect processes as diverse as gravitactic behavior and spermatogenesis. The larger Yuri isoforms contain extensive coiled-coil regions. Our previous studies indicate that one of the large isoform classes (Yuri-65) is required for formation of specialized F-actin-containing structures generated during spermatogenesis, including the so-called actin “cones” that mediate spermatid individualization. We used tandem affinity purification of a tagged version of Yuri-65 (the TAP-tagging technique) to identify proteins associated with Yuri-65 in the intact organism. Tropomyosin, primarily as the 284-residue isoform derived from the ubiquitously expressed Tropomyosin 1 gene was thus identified as a major Yuri interaction partner. Co-immunoprecipitation experiments confirmed this interaction. We have established that the stable F-actin cones of spermatogenesis contain Tropomyosin 1 (Tm1) and that in mutant yuriF64, failure of F-actin cone formation is associated with failure of Tm1 to accumulate at the cone initiation sites. In investigating possible interactions of Tm1 and Yuri in other tissues, we discovered that Tm1 and Yuri frequently colocalize with the endoplasmic reticulum. Tropomyosin has been implicated in actin-mediated membrane trafficking activity in other systems. Our findings suggest that Yuri-Tm1 complexes participate in related functions. PMID:21126519

  17. Coiled coil rich proteins (Ccrp) influence molecular pathogenicity of Helicobacter pylori.

    PubMed

    Schätzle, Sarah; Specht, Mara; Waidner, Barbara

    2015-01-01

    Pathogenicity of the human pathogen Helicobacter pylori relies on its capacity to adapt to a hostile environment and to escape the host response. Although there have been great advances in our understanding of the bacterial cytoskeleton, major gaps remain in our knowledge of its contribution to virulence. In this study we have explored the influence of coiled coil rich proteins (Ccrp) cytoskeletal elements on pathogenicity factors of H. pylori. Deletion of any of the ccrp resulted in a strongly decreased activity of the main pathogenicity factor urease. We further investigated their role using in vitro co-culture experiments with the human gastric adenocarcinoma cell line AGS modeling H. pylori - host cell interactions. Intriguingly, host cell showed only a weak "scattering/hummingbird" phenotype, in which host cells are transformed from a uniform polygonal shape into a severely elongated state characterized by the formation of needle-like projections, after co-incubation with any ccrp deletion mutant. Furthermore, co-incubation with the ccrp59 mutant resulted in reduced type IV secretion system associated activities, e.g. IL-8 production and CagA translocation/phosphorylation. Thus, in addition to their role in maintaining the helical cell shape of H. pylori Ccrp proteins influence many cellular processes and are thereby crucial for the virulence of this human pathogen. PMID:25822999

  18. Coiled tubing drilling with supercritical carbon dioxide

    DOEpatents

    Kolle , Jack J.

    2002-01-01

    A method for increasing the efficiency of drilling operations by using a drilling fluid material that exists as supercritical fluid or a dense gas at temperature and pressure conditions existing at a drill site. The material can be used to reduce mechanical drilling forces, to remove cuttings, or to jet erode a substrate. In one embodiment, carbon dioxide (CO.sub.2) is used as the material for drilling within wells in the earth, where the normal temperature and pressure conditions cause CO.sub.2 to exist as a supercritical fluid. Supercritical carbon dioxide (SC--CO.sub.2) is preferably used with coiled tube (CT) drilling equipment. The very low viscosity SC--CO.sub.2 provides efficient cooling of the drill head, and efficient cuttings removal. Further, the diffusivity of SC--CO.sub.2 within the pores of petroleum formations is significantly higher than that of water, making jet erosion using SC--CO.sub.2 much more effective than water jet erosion. SC--CO.sub.2 jets can be used to assist mechanical drilling, for erosion drilling, or for scale removal. A choke manifold at the well head or mud cap drilling equipment can be used to control the pressure within the borehole, to ensure that the temperature and pressure conditions necessary for CO.sub.2 to exist as either a supercritical fluid or a dense gas occur at the drill site. Spent CO.sub.2 can be vented to the atmosphere, collected for reuse, or directed into the formation to aid in the recovery of petroleum.

  19. Preliminary Report on the Thrombogenic Properties of Tantalum-GDC Coils

    PubMed Central

    Guglielmi, G.

    2008-01-01

    Summary In order to overcome the possibility of recanalization and compaction with Platinum GDC coils Tantalum coils were placed in three aneurysms in swine. Comparative coiling was done on the other side in each animal with platinum GDC. More stable thrombus was observed on the aneurysms coiled with Tantalum GDC. PMID:20557785

  20. Structure of Leishmania donovani coronin coiled coil domain reveals an antiparallel 4 helix bundle with inherent asymmetry.

    PubMed

    Nayak, Ashok Ranjan; Karade, Sharanbasappa Shrimant; Srivastava, Vijay Kumar; Rana, Ajay Kumar; Gupta, C M; Sahasrabuddhe, Amogh A; Pratap, J Venkatesh

    2016-07-01

    Coiled coils are ubiquitous structural motifs that serve as a platform for protein-protein interactions and play a central role in myriad physiological processes. Though the formation of a coiled coil requires only the presence of suitably spaced hydrophobic residues, sequence specificities have also been associated with specific oligomeric states. RhXXhE is one such sequence motif, associated with parallel trimers, found in coronins and other proteins. Coronin, present in all eukaryotes, is an actin-associated protein involved in regulating actin turnover. Most eukaryotic coronins possess the RhXXhE trimerization motif. However, a unique feature of parasitic kinetoplastid coronin is that the positions of R and E are swapped within their coiled coil domain, but were still expected to form trimers. To understand the role of swapped motif in oligomeric specificity, we determined the X-ray crystal structure of Leishmania donovani coronin coiled coil domain (LdCoroCC) at 2.2Å, which surprisingly, reveals an anti-parallel tetramer assembly. Small angle X-ray scattering studies and chemical crosslinking confirm the tetramer in solution and is consistent with the oligomerization observed in the full length protein. Structural analyses reveal that LdCoroCC possesses an inherent asymmetry, in that one of the helices of the bundle is axially shifted with respect to the other three. The analysis also identifies steric reasons that cause this asymmetry. The bundle adapts an extended a-d-e core packing, the e residue being polar (with an exception) which results in a thermostable bundle with polar and apolar interfaces, unlike the existing a-d-e core antiparallel homotetramers with apolar core. Functional implications of the anti-parallel association in kinetoplastids are discussed. PMID:26940672

  1. Self-assembled polymersomes formed by symmetric, asymmetric and side-chain-tethered coil-rod-coil triblock copolymers.

    PubMed

    Lin, Yung-Lung; Chang, Hung-Yu; Sheng, Yu-Jane; Tsao, Heng-Kwong

    2014-03-21

    Self-assembly behaviors of coil-rod-coil copolymers in selective solvents are explored by dissipative particle dynamics. The morphological phase diagram as a function of rod length and coil length shows five distinct types of aggregates, including spherical micelles, worm-like micelles, disk-like aggregates, honeycomb structures, and polymersomes. Small polymersomes are formed at rather poor alignment associated with monolayered rod domains. Some of the rods are even lying perpendicular to the radial direction. For symmetric copolymers (CmRxCm), the condition of vesicle formation is restricted to short coil and rod lengths. To favor the formation of CRC-polymersomes, two architecture modifications are adopted. One is to increase the coil length asymmetrically to be CmRxCn, where n > m. The other one is to tether a T-block onto the middle of the rod-block as Cm(RxTy)Cm copolymers. For those CRC-polymersomes, structural, transport, and mechanical properties of the vesicular membrane are determined, including membrane thickness, area density of coil blocks, order parameter, solvent permeability, frequency of flip-flop, membrane tension, and stretching and bending moduli. The influences of the coil length (n) and tethered block length (y) on membrane properties are examined. Finally, the mechanism of membrane fusion between CRC-polymersomes is investigated. The fusion process involves four stages and in the contact region the rods lying perpendicular to the radial direction of the polymersome play the key role. The encounter of two vesicles may result in a fused, hemifused, or non-fused polymersome. The final fate is determined by the competition between membrane tension and the steric barrier of the coil corona. The fusion outcome may change if the tension is altered by manipulating the lumen pressure. PMID:24651905

  2. The coiled-coil domain of EHD2 mediates inhibition of LeEix2 endocytosis and signaling.

    PubMed

    Bar, Maya; Sharfman, Miya; Schuster, Silvia; Avni, Adi

    2009-01-01

    Endocytosis has been suggested to be crucial for the induction of plant immunity in several cases. We have previously shown that two Arabidopsis proteins, AtEHD1 and AtEHD2, are involved in endocytosis in plant systems. AtEHD2 has an inhibitory effect on endocytosis of transferrin, FM-4-64, and LeEix2. There are many works in mammalian systems detailing the importance of the various domains in EHDs but, to date, the domains of plant EHD2 that are required for its inhibitory activity on endocytosis remained unknown. In this work we demonstrate that the coiled-coil domain of EHD2 is crucial for the ability of EHD2 to inhibit endocytosis in plants, as mutant EHD2 forms lacking the coiled-coil lost the ability to inhibit endocytosis and signaling of LeEix2. The coiled-coil was also required for binding of EHD2 to the LeEix2 receptor. It is therefore probable that binding of EHD2 to the LeEix2 receptor is required for inhibition of LeEix2 internalization. We also show herein that the P-loop of EHD2 is important for EHD2 to function properly. The EH domain of AtEHD2 does not appear to be involved in inhibition of endocytosis. Moreover, AtEHD2 influences actin organization and may exert its inhibitory effect on endocytosis through actin re-distribution. The coiled-coil domain of EHD2 functions in inhibition of endocytosis, while the EH domain does not appear to be involved in inhibition of endocytosis. PMID:19936242

  3. The Coiled-Coil Domain of EHD2 Mediates Inhibition of LeEix2 Endocytosis and Signaling

    PubMed Central

    Bar, Maya; Sharfman, Miya; Schuster, Silvia; Avni, Adi

    2009-01-01

    Endocytosis has been suggested to be crucial for the induction of plant immunity in several cases. We have previously shown that two Arabidopsis proteins, AtEHD1 and AtEHD2, are involved in endocytosis in plant systems. AtEHD2 has an inhibitory effect on endocytosis of transferrin, FM-4-64, and LeEix2. There are many works in mammalian systems detailing the importance of the various domains in EHDs but, to date, the domains of plant EHD2 that are required for its inhibitory activity on endocytosis remained unknown. In this work we demonstrate that the coiled-coil domain of EHD2 is crucial for the ability of EHD2 to inhibit endocytosis in plants, as mutant EHD2 forms lacking the coiled-coil lost the ability to inhibit endocytosis and signaling of LeEix2. The coiled-coil was also required for binding of EHD2 to the LeEix2 receptor. It is therefore probable that binding of EHD2 to the LeEix2 receptor is required for inhibition of LeEix2 internalization. We also show herein that the P-loop of EHD2 is important for EHD2 to function properly. The EH domain of AtEHD2 does not appear to be involved in inhibition of endocytosis. Moreover, AtEHD2 influences actin organization and may exert its inhibitory effect on endocytosis through actin re-distribution. The coiled-coil domain of EHD2 functions in inhibition of endocytosis, while the EH domain does not appear to be involved in inhibition of endocytosis. PMID:19936242

  4. Dimerization of the DYT6 dystonia protein, THAP1, requires residues within the coiled-coil domain.

    PubMed

    Sengel, Cem; Gavarini, Sophie; Sharma, Nutan; Ozelius, Laurie J; Bragg, D Cristopher

    2011-09-01

    Thanatos-associated [THAP] domain-containing apoptosis-associated protein 1 (THAP1) is a DNA-binding protein that has been recently associated with DYT6 dystonia, a hereditary movement disorder involving sustained, involuntary muscle contractions. A large number of dystonia-related mutations have been identified in THAP1 in diverse patient populations worldwide. Previous reports have suggested that THAP1 oligomerizes with itself via a C-terminal coiled-coil domain, raising the possibility that DYT6 mutations in this region might affect this interaction. In this study, we examined the ability of wild-type THAP1 to bind itself and the effects on this interaction of the following disease mutations: C54Y, F81L, ΔF132, T142A, I149T, Q154fs180X, and A166T. The results confirmed that wild-type THAP1 associated with itself and most of the DYT6 mutants tested, except for the Q154fs180X variant, which loses most of the coiled-coil domain because of a frameshift at position 154. However, deletion of C-terminal residues after position 166 produced a truncated variant of THAP1 that was able to bind the wild-type protein. The interaction of THAP1 with itself therefore required residues within a 13-amino acid region (aa 154-166) of the coiled-coil domain. Further inspection of this sequence revealed elements highly consistent with previous descriptions of leucine zippers, which serve as dimerization domains in other transcription factor families. Based on this similarity, a structural model was generated to predict how hydrophobic residues in this region may mediate dimerization. These observations offer additional insight into the role of the coiled-coil domain in THAP1, which may facilitate future analyses of DYT6 mutations in this region. PMID:21752024

  5. Dimerization of the DYT6 dystonia protein, THAP1, requires residues within the coiled-coil domain

    PubMed Central

    Sengel, Cem; Gavarini, Sophie; Sharma, Nutan; Ozelius, Laurie J.; Bragg, D. Cristopher

    2011-01-01

    THAP1 is a DNA binding protein that has been recently associated with DYT6 dystonia, a hereditary movement disorder involving sustained, involuntary muscle contractions. A large number of dystonia-related mutations have been identified in THAP1 in diverse patient populations worldwide. Previous reports have suggested that THAP1 oligomerizes with itself via a C-terminal coiled-coil domain, raising the possibility that DYT6 mutations in this region might affect this interaction. In this study we examined the ability of wild-type THAP1 to bind itself and the effects on this interaction of the following disease mutations: C54Y, F81L, ΔF132, T142A, I149T, Q154fs180X, and A166T. The results confirmed that wild-type THAP1 associated with itself and most of the DYT6 mutants tested, except for the Q154fs180X variant, which loses most of the coiled-coil domain due to a frameshift at position 154. However, deletion of C-terminal residues after position 166 produced a truncated variant of THAP1 that was able to bind the wild-type protein. The interaction of THAP1 with itself therefore required residues within a 13-amino acid region (aa 154–166) of the coiled-coil domain. Further inspection of this sequence revealed elements highly consistent with previous descriptions of leucine zippers, which serve as dimerization domains in other transcription factor families. Based on this similarity, a structural model was generated to predict how hydrophobic residues in this region may mediate dimerization. These observations offer additional insight into the role of the coiled-coil domain in THAP1, which may facilitate future analyses of DYT6 mutations in this region. PMID:21752024

  6. Application of anatomically shaped surface coils in MRI at 0.5 T.

    PubMed

    Doornbos, J; Grimbergen, H A; Booijen, P E; te Strake, L; Bloem, J L; Vielvoye, G J; Boskamp, E

    1986-04-01

    The construction and application of eight different MRI surface coils is described. The coils consist of an anatomically shaped copper wire loop as an antenna and a printed circuit board containing electronic components for tuning and matching. The electronic device for tuning and matching is interchangeable between the various coils. Surface coils for signal detection yield images with high signal-to-noise ratio in comparison to the usual saddle-shaped head or body coils. The sensitivity of a surface coil decreases with increasing distance between the coil and the object of interest and therefore the coils are constructed to fit the anatomical structure under examination as well as possible. The application of dedicated surface coils for superficial structures in the body extends the possibilities of the MRI system. Photographs of the coils positioned on the body and MR images of volunteers and patients are shown. PMID:3713491

  7. L1 retrotransposition requires rapid ORF1p oligomerization, a novel coiled coil-dependent property conserved despite extensive remodeling

    PubMed Central

    Naufer, M. Nabuan; Callahan, Kathryn E.; Cook, Pamela R.; Perez-Gonzalez, Cesar E.; Williams, Mark C.; Furano, Anthony V.

    2016-01-01

    Detailed mechanistic understanding of L1 retrotransposition is sparse, particularly with respect to ORF1p, a coiled coil-mediated homotrimeric nucleic acid chaperone that can form tightly packed oligomers on nucleic acids. Although the coiled coil motif is highly conserved, it is uniquely susceptible to evolutionary change. Here we studied three ORF1 proteins: a modern human one (111p), its resuscitated primate ancestor (555p) and a mosaic modern protein (151p) wherein 9 of the 30 coiled coil substitutions retain their ancestral state. While 111p and 555p equally supported retrotransposition, 151p was inactive. Nonetheless, they were fully active in bulk assays of nucleic acid interactions including chaperone activity. However, single molecule assays showed that 151p trimers form stably bound oligomers on ssDNA at <1/10th the rate of the active proteins, revealing that oligomerization rate is a novel critical parameter of ORF1p activity in retrotransposition conserved for at least the last 25 Myr of primate evolution. PMID:26673717

  8. Tailoring Supramolecular Peptide-Poly(ethylene glycol) Hydrogels by Coiled Coil Self-Assembly and Self-Sorting.

    PubMed

    Dånmark, Staffan; Aronsson, Christopher; Aili, Daniel

    2016-06-13

    Physical hydrogels are extensively used in a wide range of biomedical applications. However, different applications require hydrogels with different mechanical and structural properties. Tailoring these properties demands exquisite control over the supramolecular interactions involved. Here we show that it is possible to control the mechanical properties of hydrogels using de novo designed coiled coil peptides with different affinities for dimerization. Four different nonorthogonal peptides, designed to fold into four different coiled coil heterodimers with dissociation constants spanning from μM to pM, were conjugated to star-shaped 4-arm poly(ethylene glycol) (PEG). The different PEG-coiled coil conjugates self-assemble as a result of peptide heterodimerization. Different combinations of PEG-peptide conjugates assemble into PEG-peptide networks and hydrogels with distinctly different thermal stabilities, supramolecular, and rheological properties, reflecting the peptide dimer affinities. We also demonstrate that it is possible to rationally modulate the self-assembly process by means of thermodynamic self-sorting by sequential additions of nonpegylated peptides. The specific interactions involved in peptide dimerization thus provides means for programmable and reversible self-assembly of hydrogels with precise control over rheological properties, which can significantly facilitate optimization of their overall performance and adaption to different processing requirements and applications. PMID:27219681

  9. The Influence of Dome Size, Parent Vessel Angle, and Coil Packing Density on Coil Embolization Treatment in Cerebral Aneurysms

    NASA Astrophysics Data System (ADS)

    Frakes, David H.; Indahlastari, Aprinda; Ryan, Justin; Babiker, M. Haithem; Nair, Priya; Parthas, Varsha

    2013-11-01

    Intracranial aneurysms (ICAs) are dilated cerebral blood vessels. Treating ICAs effectively prior rupture is crucial since their association with 45% mortality rate. Embolic coiling is the most effective ICA treatment. Series of embolic coils are deployed into the aneurysm with the intent of reaching a sufficient packing density (PD) to help seal off the ICA from circulation. While coiling is effective, treatment failures have been associated with basilar tip aneurysms (BTAs), perhaps because of their geometry. The aim of this study was to examine the effect of dome size, parent vessel (PV) angle, and PD on intraaneurysmal (IA) velocity, crossneck (CN) flow and low wall shear stress (WSS) area using simulations and experiments in idealized BTA models. IA velocity and CN flow decreased after coiling, while low WSS area increased. With increasing PD, IA velocity and CN flow were further reduced, but low WSS area had a minimal change. Coil PD had the greatest impact on post-treatment flow while dome size had a greater impact than PV angle. Overall, the role of aneurysmal geometries may vary depending on treatment goal and timing e.g., high coil PD may reduce IA velocity more effectively during early aneurysmal growth when the dome size is small. Funded by the American Heart Association.

  10. N-Terminal Coiled-Coil Structure of ATPase Subunits of 26S Proteasome Is Crucial for Proteasome Function

    PubMed Central

    Inobe, Tomonao; Genmei, Reiko

    2015-01-01

    The proteasome is an essential proteolytic machine in eukaryotic cells, where it removes damaged proteins and regulates many cellular activities by degrading ubiquitinated proteins. Its heterohexameric AAA+ ATPase Rpt subunits play a central role in proteasome activity by the engagement of substrate unfolding and translocation for degradation; however, its detailed mechanism remains poorly understood. In contrast to AAA+ ATPase domains, their N-terminal regions of Rpt subunits substantially differ from each other. Here, to investigate the requirements and roles of the N-terminal regions of six Rpt subunits derived from Saccharomyces cerevisiae, we performed systematic mutational analysis using conditional knockdown yeast strains for each Rpt subunit and bacterial heterologous expression system of the base subcomplex. We showed that the formation of the coiled-coil structure was the most important for the N-terminal region of Rpt subunits. The primary role of coiled-coil structure would be the maintenance of the ring structure with the defined order. However, the coiled-coil region would be also be involved in substrate recognition and an interaction between lid and base subcomplexes. PMID:26208326

  11. The structure of human SFPQ reveals a coiled-coil mediated polymer essential for functional aggregation in gene regulation

    PubMed Central

    Lee, Mihwa; Sadowska, Agata; Bekere, Indra; Ho, Diwei; Gully, Benjamin S.; Lu, Yanling; Iyer, K. Swaminathan; Trewhella, Jill; Fox, Archa H.; Bond, Charles S.

    2015-01-01

    SFPQ, (a.k.a. PSF), is a human tumor suppressor protein that regulates many important functions in the cell nucleus including coordination of long non-coding RNA molecules into nuclear bodies. Here we describe the first crystal structures of Splicing Factor Proline and Glutamine Rich (SFPQ), revealing structural similarity to the related PSPC1/NONO heterodimer and a strikingly extended structure (over 265 Å long) formed by an unusual anti-parallel coiled-coil that results in an infinite linear polymer of SFPQ dimers within the crystals. Small-angle X-ray scattering and transmission electron microscopy experiments show that polymerization is reversible in solution and can be templated by DNA. We demonstrate that the ability to polymerize is essential for the cellular functions of SFPQ: disruptive mutation of the coiled-coil interaction motif results in SFPQ mislocalization, reduced formation of nuclear bodies, abrogated molecular interactions and deficient transcriptional regulation. The coiled-coil interaction motif thus provides a molecular explanation for the functional aggregation of SFPQ that directs its role in regulating many aspects of cellular nucleic acid metabolism. PMID:25765647

  12. BI-ground microstrip array coil vs. conventional microstrip array coil for mouse imaging at 7 tesla

    NASA Astrophysics Data System (ADS)

    Hernández, Ricardo; Terrones, M. A. López; Jakob, P. M.

    2012-10-01

    At high field strengths, the need for more efficient high frequency coils has grown. Since the radiation losses and the interaction between coil and sample increase proportionally to field strength, the quality factor (Q) and the sensitivity of the coil decrease as consequence of these negative effects. Since Zhang et al proposed in 2001 a new surface coil based on the microstrip transmission line for high frequency, different Tx-Rx phased arrays based on this concept have been already introduced in animal and whole body systems at high field strengths, each of them with different modifications in order to get better field homogeneity, SNR or isolation between coil elements in the array. All these arrays for animals systems have been built for rat imaging. One of these modifications is called BI-Ground Microstrip Array Coil (BIGMAC). The implementation of a smaller two-channel BIGMAC design for mouse imaging is studied and its performance compared to a two-channel conventional Microstrip array at 7 Tesla, the higher isolation by using BIGMAC elements in comparison with conventional Microstrip elements is shown in this work.

  13. High-resolution spot-scan electron microscopy of microcrystals of an alpha-helical coiled-coil protein.

    PubMed

    Bullough, P A; Tulloch, P A

    1990-09-01

    We describe the electron microscopy of a crystalline assembly of an alpha-helical coiled-coil protein extracted from the ootheca of the praying mantis. Electron diffraction patterns of unstained crystals show crystal lattice sampling of the coiled-coil molecular transform to a resolution beyond 1.5 A. Using a "spot-scan" method of electron imaging, micrographs of unstained crystals have been obtained that visibly diffract laser light from crystal spacings as small as 4.3 A. A projection map was calculated to 4 A using electron diffraction amplitudes and phases from computer-processed images. The projection map clearly shows modulations in density arising from the 5.1 A alpha-helical repeat, the first time this type of modulation has been revealed by electron microscopy. The crystals have p2 plane group symmetry with a = 92.4 A, b = 150.7 A, y = 92.4 degrees. Examination of tilted specimens shows that c is approximately 18 A, indicating that the unit cell is only one molecule thick. A preliminary interpretation shows tightly packed molecules some 400 A long lying with their long axes in the plane of the projection. The molecules have a coiled-coil configuration for most of their length. The possible modes of packing of the molecules in three dimensions are discussed. PMID:2398496

  14. Mechanical Transition from α-Helical Coiled-Coils to β-Sheets in Fibrin(ogen)

    PubMed Central

    Zhmurov, Artem; Kononova, Olga; Litvinov, Rustem I.; Dima, Ruxandra I.; Barsegov, Valeri; Weisel, John W.

    2012-01-01

    We characterized the α-to-β transition in α-helical coiled-coil connectors of human fibrin(ogen) molecule using biomolecular simulations of their forced elongation, and theoretical modeling. The force (F) - extension (X) profiles show three distinct regimes: (1) the elastic regime, in which the coiled-coils act as entropic springs (F < 100–125 pN; X < 7–8 nm); (2) the constant-force plastic regime, characterized by a force-plateau (F≈150 pN; X≈10–35 nm); and (3) the non-linear regime (F >175–200 pN; X > 40–50 nm). In the plastic regime, the three-stranded α-helices undergo a non-cooperative phase transition to form parallel three-stranded β-sheets. The critical extension of α-helices is 0.25 nm, and the energy difference between the α-helices and β-sheets is 4.9 kcal/mol per helical pitch. The soft α-to-β phase transition in coiled-coils might be a universal mechanism underlying mechanical properties of filamentous α-helical proteins. PMID:22953986

  15. Method of eliminating the training effect in superconducting coils by post-wind preload

    DOEpatents

    Heim, Joseph R.

    1976-01-01

    The training effect in superconducting coils is eliminated by winding the coil with a composite material that includes both a superconductor and a normal material and then applying stresses to the wound coil in the direction that electromagnetic stresses will be applied to the coil during normal use and in a magnitude greater than the calculated magnitude of the greatest electromagnetic stresses to be applied to the coil.

  16. Testing of a Single 11 T $Nb_3Sn$ Dipole Coil Using a Dipole Mirror Structure

    SciTech Connect

    Zlobin, Alexander; Andreev, Nicolai; Barzi, Emanuela; Chlachidze, Guram; Kashikhin, Vadim; Nobrega, Alfred; Novitski, Igor; Turrioni, Daniele; Karppinen, Mikko; Smekens, David

    2014-07-01

    FNAL and CERN are developing an 11 T Nb3Sn dipole suitable for installation in the LHC. To optimize coil design parameters and fabrication process and study coil performance, a series of 1 m long dipole coils is being fabricated. One of the short coils has been tested using a dipole mirror structure. This paper describes the dipole mirror magnetic and mechanical designs, and reports coil parameters and test results.

  17. Rod/Coil Block Copolyimides for Ion-Conducting Membranes

    NASA Technical Reports Server (NTRS)

    Meador, Mary Ann B.; Kinder, James D.

    2003-01-01

    Rod/coil block copolyimides that exhibit high levels of ionic conduction can be made into diverse products, including dimensionally stable solid electrolyte membranes that function well over wide temperature ranges in fuel cells and in lithium-ion electrochemical cells. These rod/coil block copolyimides were invented to overcome the limitations of polymers now used to make such membranes. They could also be useful in other electrochemical and perhaps some optical applications, as described below. The membranes of amorphous polyethylene oxide (PEO) now used in lithium-ion cells have acceptably large ionic conductivities only at temperatures above 60 C, precluding use in what would otherwise be many potential applications at lower temperatures. PEO is difficult to process, and, except at the highest molecular weights it is not very dimensionally stable. It would be desirable to operate fuel cells at temperatures above 80 C to take advantage of better kinetics of redox reactions and to reduce contamination of catalysts. Unfortunately, proton-conduction performance of a typical perfluorosulfonic polymer membrane now used as a solid electrolyte in a fuel cell decreases with increasing temperature above 80 C because of loss of water from within the membrane. The loss of water has been attributed to the hydrophobic nature of the polymer backbone. In addition, perfluorosulfonic polymers are expensive and are not sufficiently stable for long-term use. Rod/coil block copolyimides are so named because each molecule of such a polymer comprises short polyimide rod segments alternating with flexible polyether coil segments (see figure). The rods and coils can be linear, branched, or mixtures of linear and branched. A unique feature of these polymers is that the rods and coils are highly incompatible, giving rise to a phase separation with a high degree of ordering that creates nanoscale channels in which ions can travel freely. The conduction of ions can occur in the coil phase

  18. Current-Voltage Measurements in a 2G YBCO Coil

    SciTech Connect

    Rey, Christopher M; Duckworth, Robert C

    2007-01-01

    Abstract- The Oak Ridge National Laboratory in collaboration with American Superconductor Corporation and Cryomagnetics Inc. has designed, fabricated, and tested an HTS coil wound with second-generation (2G) YBCO coated conductor tape. The purpose of the HTS coil project was to study the quench characteristics in 2G YBCO coils at 77 K and lower temperatures (~ 30-45 K). These quench characteristics were investigated in both a pool boiling LN2 environment and in a conduction cooled configuration at ~ 30 K and 45 K. Transport critical current (Ic) measurements taken on the very first thermal cycle of the YBCO coil in pool boiling LN2 showed an Ic ~ 31 A corresponding to a central magnetic field of 0.32 T. The measured Ic value was consistent with the calculated value using the calculated maximum perpendicular B-field component and the measured short sample Ic at 77 K. Subsequent Ic measurements taken in the conduction cooling configuration at 34 K and 45 K, showed a steady-state Ic ~ 45-49 A and 38-44 A, respectively. These Ic values were significantly lower than the calculated value assuming a literature derived temperature dependent Ic of the 2G YBCO tape. A steady degradation was observed in the Ic of the coil with each successive thermal cycle. In addition, the coil was also pulse tested up to 1-T in non-steady state transient conditions and for ramp rates varying between 0.01 and 5 A/s. The problems and limitations encountered during testing of this new type of 2G coil is briefly discussed.

  19. Structural mapping of the coiled-coil domain of a bacterial condensin and comparative analyses across all domains of life suggest conserved features of SMC proteins.

    PubMed

    Waldman, Vincent M; Stanage, Tyler H; Mims, Alexandra; Norden, Ian S; Oakley, Martha G

    2015-06-01

    The structural maintenance of chromosomes (SMC) proteins form the cores of multisubunit complexes that are required for the segregation and global organization of chromosomes in all domains of life. These proteins share a common domain structure in which N- and C- terminal regions pack against one another to form a globular ATPase domain. This "head" domain is connected to a central, globular, "hinge" or dimerization domain by a long, antiparallel coiled coil. To date, most efforts for structural characterization of SMC proteins have focused on the globular domains. Recently, however, we developed a method to map interstrand interactions in the 50-nm coiled-coil domain of MukB, the divergent SMC protein found in γ-proteobacteria. Here, we apply that technique to map the structure of the Bacillus subtilis SMC (BsSMC) coiled-coil domain. We find that, in contrast to the relatively complicated coiled-coil domain of MukB, the BsSMC domain is nearly continuous, with only two detectable coiled-coil interruptions. Near the middle of the domain is a break in coiled-coil structure in which there are three more residues on the C-terminal strand than on the N-terminal strand. Close to the head domain, there is a second break with a significantly longer insertion on the same strand. These results provide an experience base that allows an informed interpretation of the output of coiled-coil prediction algorithms for this family of proteins. A comparison of such predictions suggests that these coiled-coil deviations are highly conserved across SMC types in a wide variety of organisms, including humans. PMID:25664627

  20. Association of polyalanine and polyglutamine coiled coils mediates expansion disease-related protein aggregation and dysfunction

    PubMed Central

    Pelassa, Ilaria; Corà, Davide; Cesano, Federico; Monje, Francisco J.; Montarolo, Pier Giorgio; Fiumara, Ferdinando

    2014-01-01

    The expansion of homopolymeric glutamine (polyQ) or alanine (polyA) repeats in certain proteins owing to genetic mutations induces protein aggregation and toxicity, causing at least 18 human diseases. PolyQ and polyA repeats can also associate in the same proteins, but the general extent of their association in proteomes is unknown. Furthermore, the structural mechanisms by which their expansion causes disease are not well understood, and these repeats are generally thought to misfold upon expansion into aggregation-prone β-sheet structures like amyloids. However, recent evidence indicates a critical role for coiled-coil (CC) structures in triggering aggregation and toxicity of polyQ-expanded proteins, raising the possibility that polyA repeats may as well form these structures, by themselves or in association with polyQ. We found through bioinformatics screenings that polyA, polyQ and polyQA repeats have a phylogenetically graded association in human and non-human proteomes and associate/overlap with CC domains. Circular dichroism and cross-linking experiments revealed that polyA repeats can form—alone or with polyQ and polyQA—CC structures that increase in stability with polyA length, forming higher-order multimers and polymers in vitro. Using structure-guided mutagenesis, we studied the relevance of polyA CCs to the in vivo aggregation and toxicity of RUNX2—a polyQ/polyA protein associated with cleidocranial dysplasia upon polyA expansion—and found that the stability of its polyQ/polyA CC controls its aggregation, localization and toxicity. These findings indicate that, like polyQ, polyA repeats form CC structures that can trigger protein aggregation and toxicity upon expansion in human genetic diseases. PMID:24497578

  1. Association of polyalanine and polyglutamine coiled coils mediates expansion disease-related protein aggregation and dysfunction.

    PubMed

    Pelassa, Ilaria; Corà, Davide; Cesano, Federico; Monje, Francisco J; Montarolo, Pier Giorgio; Fiumara, Ferdinando

    2014-07-01

    The expansion of homopolymeric glutamine (polyQ) or alanine (polyA) repeats in certain proteins owing to genetic mutations induces protein aggregation and toxicity, causing at least 18 human diseases. PolyQ and polyA repeats can also associate in the same proteins, but the general extent of their association in proteomes is unknown. Furthermore, the structural mechanisms by which their expansion causes disease are not well understood, and these repeats are generally thought to misfold upon expansion into aggregation-prone β-sheet structures like amyloids. However, recent evidence indicates a critical role for coiled-coil (CC) structures in triggering aggregation and toxicity of polyQ-expanded proteins, raising the possibility that polyA repeats may as well form these structures, by themselves or in association with polyQ. We found through bioinformatics screenings that polyA, polyQ and polyQA repeats have a phylogenetically graded association in human and non-human proteomes and associate/overlap with CC domains. Circular dichroism and cross-linking experiments revealed that polyA repeats can form--alone or with polyQ and polyQA--CC structures that increase in stability with polyA length, forming higher-order multimers and polymers in vitro. Using structure-guided mutagenesis, we studied the relevance of polyA CCs to the in vivo aggregation and toxicity of RUNX2--a polyQ/polyA protein associated with cleidocranial dysplasia upon polyA expansion--and found that the stability of its polyQ/polyA CC controls its aggregation, localization and toxicity. These findings indicate that, like polyQ, polyA repeats form CC structures that can trigger protein aggregation and toxicity upon expansion in human genetic diseases. PMID:24497578

  2. Novel Anti-Nicotine Vaccine Using a Trimeric Coiled-Coil Hapten Carrier

    PubMed Central

    Miller, Keith D.; Roque, Richard; Clegg, Christopher H.

    2014-01-01

    Tobacco addiction represents one of the largest public health problems in the world and is the leading cause of cancer and heart disease, resulting in millions of deaths a year. Vaccines for smoking cessation have shown considerable promise in preclinical models, although functional antibody responses induced in humans are only modestly effective in preventing nicotine entry into the brain. The challenge in generating serum antibodies with a large nicotine binding capacity is made difficult by the fact that this drug is non-immunogenic and must be conjugated as a hapten to a protein carrier. To circumvent the limitations of traditional carriers like keyhole limpet hemocyanin (KLH), we have synthesized a short trimeric coiled-coil peptide (TCC) that creates a series of B and T cell epitopes with uniform stoichiometry and high density. Here we compared the relative activities of a TCC-nic vaccine and two control KLH-nic vaccines using Alum as an adjuvant or GLA-SE, which contains a synthetic TLR4 agonist formulated in a stable oil-in-water emulsion. The results showed that the TCC's high hapten density correlated with a better immune response in mice as measured by anti-nicotine Ab titer, affinity, and specificity, and was responsible for a reduction in anti-carrier immunogenicity. The Ab responses achieved with this synthetic vaccine resulted in a nicotine binding capacity in serum that could prevent >90% of a nicotine dose equivalent to three smoked cigarettes (0.05 mg/kg) from reaching the brain. PMID:25494044

  3. Temperature Profile Measurements During Heat Treatment of BSCCO 2212 Coils

    SciTech Connect

    Tollestrup, Alvin; /Fermilab

    2011-04-14

    The temperature profile of two different BSCCO 2212 coils has been analyzed. The profiles are obtained from thermocouples imbedded in the windings during the heat treatment that activates the 2212. The melting and freezing of the 2212 is clearly observed. A model that describes the data and can be used to guide the processing of new coils has been developed. We have obtained the thermal history of two BSCCO coils, one from NHMFL (1) that had 10 layers of 1 mm diameter wire with 0.15 mm insulation and a second coil from OST that had 24 layers with similar insulation and conductor size. Both coils had thermocouples imbedded in the windings and excellent recordings of the temperature over the whole reaction cycle were available for analysis. There are several features that we will address in this note. Measurements have shown that the I{sub c} of the conductor is a sensitive function of its thermal history. This brings up the question of the absolute accuracy of the thermometry in the range around 882 C, the MP of 2212. The reference for the treatment profile is really related to this MP and to small deviations around it. Since the heat of fusion of 2212 is rather large, it generates a clear signal during the melting and cooling transition that automatically generates the relative temperature markers. The physics is the same as the way ice in water maintains an isothermal environment until it is all melted. A related question is the thermal response time of the coil package. The temperature cycles that are being used to optimize strand and small coils can have rapid changes easily implemented whereas a large coil may have such a large thermal time constant that the optimum cycle may not be attainable. A simple analytical model that works well for small solenoids has been developed and an ANSYS (5) program that works for larger coils with more complicated geometry has been set up but will not be discussed in this note.

  4. Method and apparatus for making superconductive magnet coils

    DOEpatents

    Borden, Albert R.

    1985-01-01

    A curved, shell-type magnet coil, adapted to be used in a superconducting magnet, is wound by providing a mandrel having a tubular cylindrical mid-portion terminating at both ends in tapered end portions formed with longitudinal slots having flexible fingers therebetween. An elongated electrical conductor is wound around an elongated oval-shaped pole island engaged with the outside of the cylindrical mid-portion, to form a multiplicity of oval-shaped turns engaged with a 180-degree segment of the mandrel. The coil turns have longitudinal portions with curved portions therebetween, engaging the tapered end portions of the mandrel. Upon completion of the winding, tapered expansion members are fully inserted into the tapered end portions, to displace the flexible fingers outwardly into a cylindrical form and to displace the curved portions of the turns into a shape conforming to such cylindrical form while also exerting increased tension upon the turns to minimize draping of the turns and to enhance the mechanical integrity of the coil. A half cylinder clamp may then be employed to clamp the coil, whereupon the coil may be solified by the use of an epoxy adhesive.

  5. Use of the Microangiographic Fluoroscope for Coiling of Intracranial Aneurysms

    PubMed Central

    Binning, Mandy J.; Orion, David; Yashar, Parham; Webb, Sharon; Ionita, Ciprian N.; Jain, Amit; Rudin, Stephen; Hopkins, L. Nelson; Siddiqui, Adnan H.; Levy, Elad I.

    2013-01-01

    BACKGROUND Neurointervention is an ever-evolving specialty with tools including microcatheters, microwires, and coils that allow treatment of pathological conditions in increasingly smaller intracranial arteries, requiring increasing accuracy. As endovascular tools evolve, so too should the imaging. OBJECTIVE To detail the use of microangiography performed with a novel fluoroscope during coiling of intracranial aneurysms in 2 separate patients and discuss the benefits and potential limitations of the technology. METHODS The microangiographic fluoroscope (MAF) is an ultra high-resolution x-ray detector with superior resolution over a small field of view. The MAF can be incorporated into a standard angiographic C-arm system for use during endovascular procedures. RESULTS The MAF was useful for improved visualization during endovascular coiling of 2 unruptured intracranial aneurysms, without adding significant time to the procedure. No significant residual aneurysm filling was identified post-coiling, and no complications occurred. CONCLUSION The MAF is a high-resolution detector developed for use in neurointerventional cases in which superior image quality over a small field of view is required. It has been used with success for coiling of 2 unruptured aneurysms at our institution. It shows promise as an important tool in improving the accuracy with which neurointerventionists can perform certain intracranial procedures. PMID:21694658

  6. Power Supply Changes for NSTX Resistive Wall Mode Coils

    SciTech Connect

    Ramakrishnan, S S.

    2013-06-28

    The National Spherical Torus Experiment (NSTX) has been designed and installed in the existing facilities at Princeton Plasma Physics Laboratory (PPPL). Most of the hardware, plant facilities, auxiliary sub-systems, and power systems originally used for the Tokamak Fusion Test Reactor (TFTR) have been used with suitable modifications to reflect NSTX needs. Prior to 2004, the NSTX power system was feeding twelve (12) circuits in the machine. In 2004 the Resistive Wall Mode (RWM) Coils were installed on the machine to correct error fields. There are six of these coils installed around the machine in the mid-plane. Since these coils need fast and accurate controls, a Switching Power Amplifier (SPA) with three sub-units was procured, installed and commissioned along with other power loop components. Two RWM Coils were connected in series and fed from one SPA sub-unit. After the initial RWM campaign, operational requirements evolved such that each of the RWM coils now requires separate power and control. Hence a second SPA with three sub-units has been procured and installed. The second unit is of improved design and has the controls and power components completely isolated. The existing thyristor rectifier is used as DC Link to both of the Switching Power Amplifiers. The controls for the RWM are integrated into the overall computer control of the DC Power systems for NSTX. This paper describes the design changes in the RWM Power system for NSTX.

  7. Optimization design of the coil of the eddy current sensor

    NASA Astrophysics Data System (ADS)

    Pu, Tiecheng; Fan, Shangchun

    2006-11-01

    An eddy current sensor is usually used to measure the departure of a shaft from its axes, in order to avoid destroying the system because of collision. The design of the coil as the sense organ of an eddy current sensor is to search a set of proper sizes (includes the outer radius, the inner radius and tallness of the coil) in which the quality factor and the grads of magnetic field strength is great as soon as possible but the length of the lead is not much long. So an optimization function is introduced here for efficient design. This function is direct ratio with the quality factor of the core and the magnetic grads product by the coil and inverse ratio with the lead length. The proportions of three parameters can be changed according to the instance. When the value of the function reaches the maximum, the sizes of coil are the anticipant optimal sizes and the integration capability of the coil is at the high-point. To search the maximum of the function, the genetic algorithm is adopted. The simulation result by Matlab proves the practicability of the method.

  8. Thermal Fatigue Test Apparatus for Large Superconducting Coils

    NASA Astrophysics Data System (ADS)

    Kephart, J. T.; Fitzpatrick, B. K.; Chen, J.; Lopez, J.; Millar, M.; Wong, L.; Zimmerman, M.; Kostic, J.; Rakus, C.

    2008-03-01

    ABSTRACT The United States Navy has a continued interest in the development of High Temperature Superconductivity (HTS) to provide power dense, efficient propulsion and electrical power generation. These machines have large HTS rotor coils that will undergo many thermal cycles during the life of the ship. Thermal fatigue tests for large coils are necessary to understand any degradation and life issues that could arise. The Naval Surface Warfare Center Carderock Division (NSWCCD) has sponsored Rowan University to design and build a device that will assist in the thermal fatigue testing of a superconducting coil. It was designed to be autonomous with programmable cool down and warm-up rates and varying temperature from ambient temperature (300K) down to 77K. A typical test would include thermally cycling a coil a specified number of times, then performing a critical current test on the coil and repeating the test cycle as many times as desired. This paper introduces the thermal cycling test setup and presents preliminary calibration data.

  9. Emphysema: coiling up the lungs, trick or treat?

    PubMed

    Bezzi, M; Mondoni, M; Sorino, C; Solidoro, P

    2015-08-01

    Lung volume reduction coil (LVRC) treatment is a minimally-invasive technique planned to achieve an improvement of exercise capacity and pulmonary function in subjects with advanced emphysema and hyperinflation. It has been proposed together with other bronchoscopic lung volume reduction approaches to reduce lung hyperinflation in emphysema as less invasive alternatives to LVRS and are currently under clinical investigation. Following the successful early experiences in previous pilot trials, recent studies allow further investigation into the feasibility, safety and efficacy of LVR coil treatment in a multi-center setting in a larger group of patients. According to this studies we can state that LVR coil treatment results in significant clinical improvements in patients with severe emphysema, in multicenter analysis, with a good safety profile and sustained results for up to 1 year. The literature on endobronchial coils continues to look promising with an acceptable safety profile, and positive long-term follow-up data are certainly more and more available. However, further well-designed, blinded, placebo (or sham) controlled trials, and even randomized trials against LVRS (lung volume reduction surgery), are needed before routine clinical use can be recommended. This is true not only for endobronchial coils, but also for the whole field of bronchoscopic lung volume reduction. PMID:27427120

  10. New Ideas and Tools for Designing Optimized Stellarator Coils

    NASA Astrophysics Data System (ADS)

    Valanju, Prashant; Miner, William; Brooks, Art; Pomphrey, Neil; Hirshman, Steven; Berry, Lee

    2000-10-01

    We present new ideas and a complete collection of tools for solving the difficult problem of designing, under various physics and engineering constraints, optimized coils for low aspect ratio stellarators. These include various upgrades to enhance the abilities of the NESCOIL code to obtain a surface current distribution which reproduces the desired plasma shape. We have also added the capability to target, within NESCOIL, the resonant errors produced by the current sheet. For the next design step of obtaining filamentary coils from the surface currents, we present a novel use of the genetic algorithm which improves and hastens the search for a deep minimum in the optimization criteria within a very large parameter space. For the final design step of obtaining finite-size coils from the filaments, we present a series of ideas and codes to allow efficient targeting of various engineering constraints. Taken together, this suite of codes has enabled us to successfully design saddle coils for the optimized quasi-axisymmetric (QA) plasma under consideration for the National Compact Stellarator Experiment (NCSX) and modular coils for the quasi-omnigenous (QO) configuration.

  11. Method and apparatus for making superconductive magnet coils

    DOEpatents

    Borden, A.R.

    1983-11-07

    A curved, shell-type magnet coil, adapted to be used in a superconducting magnet, is wound by providing a mandrel having a tubular cylindrical mid-portion terminating at both ends in tapered end portions formed with longitudinal slots having flexible fingers therebetween. An elongated electrical conductor is wound around an elongated oval-shaped pole island engaged with the outside of the cylindrical mid-portion, to form a multiplicity of oval-shaped turns engaged with a 180-degree segment of the mandrel. The coil turns have longitudinal portions with curved portions therebetween, engaging the tapered end portions of the mandrel. Upon completion of the winding, tapered expansion members are fully inserted into the tapered end portions, to displace the flexible fingers outwardly into a cylindrical form and to displace the curved portions of the turns into a shape conforming to such cylindrical form while also exerting increased tension upon the turns to minimize draping of the turns and to enhance the mechanical integrity of the coil. A half cylinder clamp may then be employed to clamp the coil, whereupon the coil may be solidified by the use of an epoxy adhesive.

  12. Embolization of the Gastroduodenal Artery Before Selective Internal Radiotherapy: A Prospectively Randomized Trial Comparing Standard Pushable Coils with Fibered Interlock Detachable Coils

    SciTech Connect

    Dudeck, Oliver Bulla, Karsten; Wieners, Gero; Ruehl, Ricarda; Ulrich, Gerd; Amthauer, Holger; Ricke, Jens; Pech, Maciej

    2011-02-15

    The purpose of this study was compare embolization of the gastroduodenal artery (GDA) using standard pushable coils with the Interlock detachable coil (IDC), a novel fibered mechanically detachable long microcoil, in patients scheduled for selective internal radiotherapy (SIRT). Fifty patients (31 male and 19 female; median age 66.6 {+-} 8.1 years) were prospectively randomized for embolization using either standard coils or IDCs. Procedure time, radiation dose, number of embolization devices, complications, and durability of vessel occlusion at follow-up angiography were recorded. The procedures differed significantly in time (14:32 {+-} 5:56 min for standard coils vs. 2:13 {+-} 1:04 min for IDCs; p < 0.001); radiation dose for coil deployment (2479 {+-} 1237 cGycm Superscript-Two for standard coils vs. 275 {+-} 268 cGycm Superscript-Two for IDCs; p < 0.001); and vessel occlusion (17:18 {+-} 6:39 min for standard coils vs. 11:19 {+-} 7:54 min for IDCs; p = 0.002). A mean of 6.2 {+-} 1.8 coils (n = 27) were used in the standard coil group, and 1.3 {+-} 0.9 coils (p < 0.0001) were used in the IDC group (n = 23) because additional pushable coils were required to achieve GDA occlusion in 4 patients. In 2 patients, the IDC could not be deployed through a Soft-VU catheter. One standard coil dislodged in the hepatic artery and was retrieved. Vessel reperfusion was noted in only 1 patient in the standard coil group. Controlled embolization of the GDA with fibered IDCs was achieved more rapidly than with pushable coils. However, vessel occlusion may not be obtained using a single device only, and the use of sharply angled guiding catheters hampered coil pushability.

  13. Special Coils Development at the National High Magnetic Field Laboratory in Toulouse

    NASA Astrophysics Data System (ADS)

    Béard, J.; Billette, J.; Frings, P.; Suleiman, M.; Lecouturier, F.

    2013-03-01

    The Laboratoire National des Champs Magnétiques Intenses (LNCMI) develops different types of coils suited to specific experiments. We present some recent developments on magnet design. Several coils are dedicated to experiments in large scale facilities in France and Switzerland. A 30 T split-pair coil for X-rays diffraction and one 40 T coil for plasma physics at the LULI, two 30 T coils with axial access (one with an conical bore) for X-ray diffraction and absorption experiments. A 40 T wide angle conical access solenoid with a high duty-cycle for neutron scattering at the ILL is being constructed. For use at the installation in Toulouse we have developed, apart from our standard 60 and 70 T coils, several special coils: a coil with a long optical path with 30 T transverse magnetic field and a 90 T long pulse dual coil system.

  14. High field volume coil with unbalance current distribution for MRI applications of rodents

    NASA Astrophysics Data System (ADS)

    Marrufo, O. R.; Hernández, J.; Rodríguez, A. O.

    2010-12-01

    The development of transceiver volume coils for high field MRI is still a very dynamic field of investigation and development Temnikov has been recently proposed a new volume coil design, similar to the to the gradiometer coil. It is also claimed that it is possible to individually tune it with a single chip capacitor. This motivated the development of a coil prototype based on this idea for whole-body MRI of rodents at 7 Tesla. Electromagnetic simulations of the RF field generated by this coil design were previously performed to study its properties. Electromagnetic simulations were also conducted for a standard birdcage coil with similar dimensions for fare comparison. In all numerical simulations, an unbalanced currents distribution was assumed by applying half the current intensity to designated legs. This coil design operated in the transceiver mode and was linear-driven. The coil size was manufactured to accommodate small rodents. Numerical simulations showed a field uniformity improvement of our coil over the standard birdcage coil. A popular birdcage coil was also constructed to compare their performances. Phantom and rat images were acquired for both volume coils to prove the viability of this coil design for high field MRI applications and standard spin echo pulse sequences Thus, these preliminary results make this coil design a good candidate for MRI and MRS applications of high magnetic fields.

  15. Uniformly wound superconducting coil and method of making same

    DOEpatents

    Mookerjee, S.; Weijun, S.; Yager, B.

    1994-03-08

    A coil of superconducting wire for a superconducting magnet is described having a relatively dense and uniformly spaced winding to enhance the homogeneity and strength of the magnetic field surrounding the coil and a method of winding the same wherein the mandrel used to wind said coil comprises removable spacers and retainers forming a plurality of outwardly opening slots, each of said slots extending generally about the periphery of the mandrel and being sized to receive and outwardly align and retain successive turns of the superconducting wire within each slot as the wire is wound around and laterally across the mandrel to form a plurality of wire ribbons of a predetermined thickness laterally across the mandrel. 8 figures.

  16. Coil embolization of a congenital orbital varix in a dog.

    PubMed

    Adkins, Elizabeth A; Ward, Daniel A; Daniel, Gregory B; Wooten, Paul T

    2005-12-15

    A 10-week-old Labrador Retriever was examined because of a swelling above the left eye. Ophthalmic examination revealed a tubular, light-pink, slightly raised lesion of the left conjunctiva that extended from the limbus to the fornix and into the dorsal eyelid. The lesion affected the entire margin of the dorsal eyelid and extended 2 cm dorsal to the eyelid margin. With compression of the left jugular vein, the exophthalmos worsened immediately and the subconjunctival and eyelid lesion enlarged. Results of ultrasonography, computed tomography, and contrast venography were consistent with a diagnosis of an orbital varix. Coil embolization was elected for treatment of the varix to prevent the pain and morbidity associated with an orbitotomy. Coils were introduced through a 22-gauge IV catheter inserted through the upper eyelid into the varix. The only complication was moderately severe orbital swelling. The owners reported that the lesion had resolved by 2 weeks after coil embolization. PMID:16379632

  17. Miniature coils for producing pulsed inplane magnetic fields for nanospintronics

    SciTech Connect

    Pawliszak, Łukasz; Zgirski, Maciej

    2015-03-15

    Nanospintronic and related research often requires the application of quickly rising magnetic field pulses in the plane of the studied planar structure. We have designed and fabricated sub-millimeter-sized coils capable of delivering pulses of the magnetic field up to ∼500 Oe in the plane of the sample with the rise time of the order of 10 ns. The placement of the sample above the coil allows for easy access to its surface with manipulators or light beams for, e.g., Kerr microscopy. We use the fabricated coil to drive magnetic domain walls in 1 μm wide permalloy wires and measure magnetic domain wall velocity as a function of the applied magnetic field.

  18. Modeling coiled-tubing velocity strings for gas wells

    SciTech Connect

    Martinez, J.; Martinez, A.

    1998-02-01

    Because of its ability to prolong well life, its relatively low expense, and the relative ease with which it is installed, coiled tubing has become a preferred remedial method of tubular completion for gas wells. Of course, the difficulty in procuring wireline-test data is a drawback to verifying the accuracy of the assumptions and predictions used for coiled-tubing selection. This increases the importance of the prediction-making process, and, as a result, places great emphasis on the modeling methods that are used. This paper focuses on the processes and methods for achieving sound multiphase-flow predictions by looking at the steps necessary to arrive at coiled-tubing selection. Furthermore, this paper examines the variables that serve as indicators of the viability of each tubing size, especially liquid holdup. This means that in addition to methodology, emphasis is placed on the use of a good wellbore model. The computer model discussed is in use industry wide.

  19. Uniformly wound superconducting coil and method of making same

    DOEpatents

    Mookerjee, Sumit; Weijun, Shen; Yager, Billy

    1994-01-01

    A coil of superconducting wire for a superconducting magnet having a relaely dense and uniformly spaced winding to enhance the homogeneity and strength of the magnetic field surrounding the coil and a method of winding the same wherein the mandrel used to wind said coil comprises removable spacers and retainers forming a plurality of outwardly opening slots, each of said slots extending generally about the periphery of the mandrel and being sized to receive and outwardly align and retain successive turns of the superconducting wire within each slot as the wire is wound around and laterally across the mandrel to form a plurality of wire ribbons of a predetermined thickness laterally across the mandrel.

  20. Thermosyphon coil arrangement for heat pump outdoor unit

    DOEpatents

    Draper, R.

    1984-05-22

    For a heat pump, the outdoor unit is provided with a coil and a refrigerant flow arrangement there for which is such that in the heating mode of operation of the heat pump they operate in a thermosyphon fashion. The coil has a feed portion and an exit portion leading to a separator drum from which liquid refrigerant is returned through downcomer line for recirculation to the feed portion. The coil is tilted upwardly from entry to exit by the angle alpha to enhance the clearance of the two phases of refrigerant from each other in the heating mode of operation. There is no thermosyphon function in the cooling mode of operation. 9 figs.

  1. Voice Coil Percussive Mechanism Concept for Hammer Drill

    NASA Technical Reports Server (NTRS)

    Okon, Avi

    2009-01-01

    A hammer drill design of a voice coil linear actuator, spring, linear bearings, and a hammer head was proposed. The voice coil actuator moves the hammer head to produce impact to the end of the drill bit. The spring is used to store energy on the retraction and to capture the rebound energy after each impact for use in the next impact. The maximum actuator stroke is 20 mm with the hammer mass being 200 grams. This unit can create impact energy of 0.4 J with 0.8 J being the maximum. This mechanism is less complex than previous devices meant for the same task, so it has less mass and less volume. Its impact rate and energy are easily tunable without changing major hardware components. The drill can be driven by two half-bridges. Heat is removed from the voice coil via CO2 conduction.

  2. Coil and iron design for SSC 50 mm magnet

    SciTech Connect

    Gupta, R.C.; Kahn, S.A.; Morgan, G.H.

    1990-01-01

    In this paper we present the design of the two dimensional coil and iron cross section, referred to as DSX201/W6733, for the 50 mm aperture dipole magnet being built at the Brookhaven National Laboratory for the Superconducting Super Collider (SSC). The computed values of the allowed field harmonics as a function of current, the quench performance predictions, the stored energy calculations, the effect of random errors on the coil placement and the Lorentz forces on the coil will be presented. The yoke has been optimized to reduce iron saturation effects on the field harmonics. We shall present the summary of this design which will include the expected overall performance of this cross section. 4 refs., 8 figs., 12 tabs.

  3. Volume coil based on hybridized resonators for magnetic resonance imaging

    NASA Astrophysics Data System (ADS)

    Jouvaud, C.; Abdeddaim, R.; Larrat, B.; de Rosny, J.

    2016-01-01

    We present an electromagnetic device based on hybridization of four half-wavelength dipoles which increases the uniformity and the strength of the radio-frequency (RF) field of a Magnetic Resonant Imaging (MRI) apparatus. Numerical results show that this Hybridized Coil (HC) excited with a classical loop coil takes advantage of the magnetic hybrid modes. The distribution of the RF magnetic field is experimentally confirmed on a 7-T MRI with a gelatin phantom. Finally, the HC is validated in vivo by imaging the head of an anesthetized rat. We measure an overall increase of the signal to noise ratio with up to 2.4 fold increase in regions of interest far from the active loop coil.

  4. Thermosyphon coil arrangement for heat pump outdoor unit

    DOEpatents

    Draper, Robert

    1984-01-01

    For a heat pump, the outdoor unit is provided with a coil and a refrigerant flow arrangement therefor which is such that in the heating mode of operation of the heat pump they operate in a thermosyphon fashion. The coil 32 has a feed portion 30 and an exit portion 34 leading to a separator drum 36 from which liquid refrigerant is returned through downcomer line 42 for recirculation to the feed portion. The coil is tilted upwardly from entry to exit by the angle alpha to enhance the clearance of the two phases of refrigerant from each other in the heating mode of operation. There is no thermosyphon function in the cooling mode of operation.

  5. Coil performances of superconducting cables for ac applications

    SciTech Connect

    Lacaze, A.; Laumond, Y.; Bonnet, P. ); Fevrier, A.; Verhaege, T. ); Ansart, A. )

    1992-01-01

    Multifilamentary superconducting wires with a greatly reduced level of losses have been produced with unit lengths of several tens of kilometers. The authors manufactured three industrial wires named L, T, and S, comprising respectively 377982, 597102 and 920304 filaments, 0.14 {mu}m in diameter with a 0.13 {mu}m spacing between filaments. The characteristics of these wires are already published and the authors have tested coils made with (6 + 1) cables of each kind of strands (L, T, and S), the results are also published in A.S.C.90. In this paper, the results of coil tests performed with a (6 + 1) of L strand from 0 to 100 Hz are given, as well as the first results of the tests of coils made with two stage cables (6 + 1){sup 2} with L, T and S strands.

  6. Lessons from design and manufacture of US LCT coils

    SciTech Connect

    Kibbe, R.K.; Haubenreich, P.N.; Gerwig, C.H.; Hackley, D.S.; Sanger, P.A.; Young, J.L.

    1981-01-01

    In the international Large Coil Task (LCT), US industrial teams have finished one coil and are nearing completion of two others. Of the lessons learned, the ones that apply most widely to advanced technology development have to do with the difficulty of scheduling progress in a novel undertaking. Reasons for schedule stretch-out are discussed in this paper. The major factor in the LCT was the discovery by all participants of more technical problems than could have been foreseen. Also, the effort implicit in the demands for reliability and quality were greater than appreciated at first. Other factors were changes in requirements during the projects and restrictions on effort due to budget constraints. LCT experience should be particularly useful in scheduling development of superconducting coils for tokamak fusion reactors.

  7. Design and Fabrication of Racetrack Coil Accelerator Magnets

    SciTech Connect

    Chow, K.; Dietderich, D.R.; Gourlay, S.A.; Gupta, R.; Harnden, W.; Lietzke, A.; McInturff, A.D.; Millos, G.; Morrison, L.; Morrison, M.; Scanlan, R.M.

    1998-11-11

    Most accelerator magnets for applications in the field range up to 9 T utilize NbTi superconductor and a cosine theta coil design. For fields above 9 T, it is necessary to use Nb{sub 3}Sn or other strain sensitive materials, and other coil geometries that are more compatible with these materials must be considered. This paper describes their recent efforts to design a series of racetrack coil magnets that will provide experimental verification of this alternative magnet design for a dual aperture dipole magnet with the goal of reaching a field level of 15 T, will be described. The experimental program, which consists of a series of steps leading to a high field accelerator quality magnet, will be presented. Fabrication of a racetrack dipole magnet utilizing Nb{sub 3}Sn superconductor and a wind and react approach will be presented.

  8. Performance of a 12-coil superconducting 'bumpy torus' magnet facility.

    NASA Technical Reports Server (NTRS)

    Roth, J. R.; Holmes, A. D.; Keller, T. A.; Krawczonek, W. M.

    1972-01-01

    The NASA-Lewis 'bumpy torus' facility consists of 12 superconducting coils, each 19 cm ID and capable of 3.0 tesla on their axes. The coils are equally spaced around a toroidal array with a major diameter of 1.52 m, and are mounted with the major axis of the torus vertical in a single vacuum tank 2.6 m in diameter. Final shakedown tests of the facility mapped out its magnetic, cryogenic, vacuum, mechanical, and electrical performance. The facility is now ready for use as a plasma physics research facility. A maximum magnetic field on the magnetic axis of 3.23 teslas has been held for a period of more than sixty minutes without a coil normalcy.

  9. Active Protection of an MgB2 Test Coil

    PubMed Central

    Park, Dong Keun; Hahn, Seungyong; Bascuñán, Juan; Iwasa, Yukikazu

    2011-01-01

    This paper presents results of a study, experimental and computational, of a detect-and-activate-the-heater protection technique applied to a magnesium diboride (MgB2) test coil operated in semi-persistent mode. The test coil with a winding ID of 25 cm and wound with ~500-m long reacted MgB2 wire was operated at 4.2 K immersed in a bath of liquid helium. In this active technique, upon the initiation of a “hot spot” of a length ~10 cm, induced by a “quench heater,” a “protection heater” (PH) of ~600-cm long planted within the test coil is activated. The normal zone created by the PH is large enough to absorb the test coil’s entire initial stored energy and still keeps the peak temperature within the winding below ~260 K. PMID:22081754

  10. Rapid Covalent Fluorescence Labeling of Membrane Proteins on Live Cells via Coiled-Coil Templated Acyl Transfer.

    PubMed

    Reinhardt, Ulrike; Lotze, Jonathan; Mörl, Karin; Beck-Sickinger, Annette G; Seitz, Oliver

    2015-10-21

    Fluorescently labeled proteins enable the microscopic imaging of protein localization and function in live cells. In labeling reactions targeted against specific tag sequences, the size of the fluorophore-tag is of major concern. The tag should be small to prevent interference with protein function. Furthermore, rapid and covalent labeling methods are desired to enable the analysis of fast biological processes. Herein, we describe the development of a method in which the formation of a parallel coiled coil triggers the transfer of a fluorescence dye from a thioester-linked coil peptide conjugate onto a cysteine-modified coil peptide. This labeling method requires only small tag sequences (max 23 aa) and occurs with high tag specificity. We show that size matching of the coil peptides and a suitable thioester reactivity allow the acyl transfer reaction to proceed within minutes (rather than hours). We demonstrate the versatility of this method by applying it to the labeling of different G-protein coupled membrane receptors including the human neuropeptide Y receptors 1, 2, 4, 5, the neuropeptide FF receptors 1 and 2, and the dopamine receptor 1. The labeled receptors are fully functional and able to bind the respective ligand with high affinity. Activity is not impaired as demonstrated by activation, internalization, and recycling experiments. PMID:26367072

  11. Mini-coil for magnetic stimulation in the behaving primate.

    PubMed

    Tischler, Hadass; Wolfus, Shuki; Friedman, Alexander; Perel, Eli; Pashut, Tamar; Lavidor, Michal; Korngreen, Alon; Yeshurun, Yosef; Bar-Gad, Izhar

    2011-01-15

    Transcranial magnetic stimulation (TMS) is rapidly becoming a leading method in both cognitive neuroscience and clinical neurology. However, the cellular and network level effects of stimulation are still unclear and their study relies heavily on indirect physiological measurements in humans. Direct electrophysiological studies of the effect of magnetic stimulation on neuronal activity in behaving animals are severely limited by both the size of the stimulating coils, which affect large regions of the animal brain, and the large artifacts generated on the recording electrodes. We present a novel mini-coil which is specifically aimed at studying the neurophysiological mechanism of magnetic stimulation in behaving primates. The mini-coil fits into a chronic recording chamber and provides focal activation of brain areas while enabling simultaneous extracellular multi-electrode recordings. We present a comparison of this coil to a commercial coil based on the theoretical and recorded magnetic fields and induced electric fields they generate. Subsequently, we present the signal recorded in the behaving primate during stimulation and demonstrate the ability to extract the spike trains of multiple single units from each of the electrodes with minimal periods affected by the stimulus artifact (median period <2.5 ms). The directly recorded effect of the magnetic stimulation on cortical neurons is in line with peripheral recordings obtained in humans. This novel mini-coil is a key part of the infrastructure for studying the neurophysiological basis of magnetic stimulation, thereby enabling the development and testing of better magnetic stimulation tools and protocols for both neuroscientists and clinicians. PMID:20974177

  12. Mechanically Induced Helix-Coil Transition in Biopolymer Networks

    PubMed Central

    Courty, Sebastien; Gornall, Joanne L.; Terentjev, Eugene M.

    2006-01-01

    The quasi-equilibrium evolution of the helical fraction occurring in a biopolymer network (gelatin gel) under an applied stress has been investigated by observing modulation in its optical activity. Its variation with the imposed chain extension is distinctly nonmonotonic and corresponds to the transition of initially coiled strands to induced left-handed helices. The experimental results are in qualitative agreement with theoretical predictions of helices induced on chain extension. This new effect of mechanically stimulated helix-coil transition has been studied further as a function of the elastic properties of the polymer network: crosslink density and network aging. PMID:16239334

  13. The ``nautilus``: A method of winding disc coils

    SciTech Connect

    Williams, J.E.C.; Holowinski, M.; Bobrov, E.S.; Leupold, M.J.; Pourrahimi, S.; Stejskal, V.J.; Szczepanowski, A.

    1996-07-01

    The so called nautilus is a winding form in the shape of a double helical spiral onto which a stiff conductor is wound. The conductor is thus bent to a radius smaller than the desired local curvature. When the conductor is allowed to relax from the nautilus form, the required radius of curvature is achieved everywhere. The stiffness in the axial direction is small and compression of the wound coil to a disc is easy. The coil is tightened to a compact disk by pulling the outer tails.

  14. Control coil arrangement for a rotating machine rotor

    DOEpatents

    Shah, Manoj R.; Lewandowsk, Chad R.

    2001-07-31

    A rotating machine (e.g., a turbine, motor or generator) is provided wherein a fixed solenoid or other coil configuration is disposed adjacent to one or both ends of the active portion of the machine rotor for producing an axially directed flux in the active portion so as to provide planar axial control at single or multiple locations for rotor balance, levitation, centering, torque and thrust action. Permanent magnets can be used to produce an axial bias magnetic field. The rotor can include magnetic disks disposed in opposed, facing relation to the coil configuration.

  15. Control Coil Arrangement for a Rotating Machine Rotor

    SciTech Connect

    Shah, Manoj R.; Lewandowski, Chad R.

    1999-05-05

    A rotating machine (e.g., a turbine, motor or generator) is provided wherein a fixed solenoid or other coil configuration is disposed adjacent to one or both ends of the active portion of the machine rotor for producing an axially directed flux in the active portion so as to provide planar axial control at single or multiple locations for rotor balance, levitation, centering, torque and thrust action. Permanent magnets can be used to produce an axial bias magnetic field. The rotor can include magnetic disks disposed in opposed, facing relation to the coil configuration.

  16. Endovascular coil embolization of unruptured posterior communicating artery aneurysm.

    PubMed

    Binning, Mandy; Hakma, Zakaria; Veznedaroglu, Erol

    2014-07-01

    The patient is a 60-year-old woman who presented to her primary care physician with new onset of headache. She was neurologically intact without cranial nerve deficit. An outpatient CT angiogram (CTA) revealed no subarachnoid hemorrhage, but showed a right-sided posterior communicating artery aneurysm measuring 11 mm by 10 mm. Digitally subtracted cerebral angiography confirmed these measurements and showed that the aneurysm was amenable to endovascular coil embolization. The patient underwent aneurysm coiling without complication and was discharged to home on postoperative Day 1. The video can be found here: http://youtu.be/MjOc3Zpv2K8 . PMID:24983726

  17. SERPENTINE COIL TOPOLOGY FOR BNL DIRECT WIND SUPERCONDUCTING MAGNETS.

    SciTech Connect

    PARKER, B.; ESCALLIER, J.

    2005-05-16

    Serpentine winding, a recent innovation developed at BNL for direct winding superconducting magnets, allows winding a coil layer of arbitrary multipolarity in one continuous winding process and greatly simplifies magnet design and production compared to the planar patterns used before. Serpentine windings were used for the BEPC-II Upgrade and JPARC magnets and are proposed to make compact final focus magnets for the EC. Serpentine patterns exhibit a direct connection between 2D body harmonics and harmonics derived from the integral fields. Straightforward 2D optimization yields good integral field quality with uniformly spaced (natural) coil ends. This and other surprising features of Serpentine windings are addressed in this paper.

  18. Eddy current gauge for monitoring displacement using printed circuit coil

    DOEpatents

    Visioli, Jr., Armando J.

    1977-01-01

    A proximity detection system for non-contact displacement and proximity measurement of static or dynamic metallic or conductive surfaces is provided wherein the measurement is obtained by monitoring the change in impedance of a flat, generally spiral-wound, printed circuit coil which is excited by a constant current, constant frequency source. The change in impedance, which is detected as a corresponding change in voltage across the coil, is related to the eddy current losses in the distant conductive material target. The arrangement provides for considerable linear displacement range with increased accuracies, stability, and sensitivity over the entire range.

  19. Multi-turn, printed surface coil inductance, and Q optimization.

    PubMed

    Raad, A; Kan, S

    1993-03-01

    Wheeler's empirical inductance formula for a multi-turn, close-wound flat coil shows that the maximum inductance for a given length of wire occurs when the outer to inner radius ratios is equal to 15/7. A similar expression is proposed for a flat, printed spiral for surface coil antenna design by modifying one of the coefficients in Wheeler's formula. Measured inductance is in good agreement with this new formula and the optimum radius ratio for maximum inductance or Q is now of the order of 9/5. PMID:8450749

  20. A Preformed Scleral Search Coil for Measuring Mouse Eye Movements

    PubMed Central

    Kaneko, Chris R. S.; Rosenfeld, Sam; Fontaine, Ethan; Markov, Alex; Phillips, James O.; Yarno, John

    2010-01-01

    Mice are excellent subjects for use of genetic-manipulation techniques to study the basis of pathological and normal physiology and behavior; however behavioral analyses of associated phenotypes is often limited. To improve the accuracy and specificity of repeated measurements of vestibular function, we developed a miniaturized, contact-lens scleral search coil to measure mouse eye movements. We describe the physical attributes and document its functionality by measuring vestibulo-ocular responses in normal mice. This coil should greatly improve the sensitivity and documentation of vestibular dysfunction in mouse models of pathology and dysfunction while allowing screening of significant numbers of subjects. PMID:20817027

  1. Stabilization of the vertical instability by non-axisymmetric coils

    NASA Astrophysics Data System (ADS)

    Turnbull, A. D.; Reiman, A. H.; Lao, L. L.; Cooper, W. A.; Ferraro, N. M.; Buttery, R. J.

    2016-08-01

    In a published Physical Review Letter (Reiman 2007 Phys. Rev. Lett. 99 135007), it was shown that axisymmetric (or vertical) stability can be improved by placing a set of parallelogram coils above and below the plasma oriented at an angle to the constant toroidal planes. The physics of this stabilization can be understood as providing an effective additional positive stability index. The original work was based on a simplified model of a straight tokamak and is not straightforwardly applicable to a finite aspect ratio, strongly shaped plasma such as in DIII-D. Numerical calculations were performed in a real DIII-D -like configuration to provide a proof of principal that 3-D fields can, in fact raise the elongation limits as predicted. A four field period trapezioid-shaped coil set was developed in toroidal geometry and 3D equilibria were computed using trapezium coil currents of 10 kA , 100 kA , and 500 kA . The ideal magnetohydrodynamics growth rates were computed as a function of the conformal wall position for the n = 0 symmetry-preserving family. The results show an insignificant relative improvement in the stabilizing wall location for the two lower coil current cases, of the order of 10‑3 and less. In contrast, the marginal wall position is increased by 7% as the coil current is increased to 500 kA , confirming the main prediction from the original study in a real geometry case. In DIII-D the shift in marginal wall position of 7% would correspond to being able to move the existing wall outward by 5 to 10 cm. While the predicted effect on the axisymmetric stability is real, it appears to require higher coil currents than could be provided in an upgrade to existing facilities. Additional optimization over the pitch of the coils, the number of field periods and the coil positions, as well as plasma parameters, such as the internal inductivity {{\\ell}\\text{i}} , β , and {{q}95} would mitigate this but seem unlikely to change the conclusion.

  2. Field Quality Optimization in a Common Coil Magnet Design

    SciTech Connect

    Gupta, Ramesh; Ramberger, Suitbert

    1999-09-01

    This paper presents the results of initial field quality optimization of body and end harmonics in a 'common coil magnet design'. It is shown that a good field quality, as required in accelerator magnets, can be obtained by distributing conductor blocks in such a way that they simulate an elliptical coil geometry. This strategy assures that the amount of conductor used in this block design is similar to that is used in a conventional cosine theta design. An optimized yoke that keeps all harmonics small over the entire range of operation using a single power supply is also presented. The field harmonics are primarily optimized with the computer program ROXIE.

  3. Transcatheter coil closure of large patent ductus arteriosus with 0.052-inch Gianturco coils using myocardial biopsy forceps in a 70-year-old woman.

    PubMed

    Tokuda, Y; Matsumoto, M; Suda, K; Matsumura, M

    2001-06-01

    Small patent ductus arteriosus is generally closed in children using a transcatheter coil. This is done less often in older patients or those with large patent ductus arteriosus. We report successful antegrade transcatheter coil closure of patent ductus arteriosus in a 70-year-old woman. Into the patent ductus arteriosus, using flexible myocardial biopsy forceps, we placed two large 0.052-inch Gianturco coils, which were easily used as multipurpose vascular occlusion coils. The forceps and the coils were readily available and provided complete occlusion. Other delivery devices cannot deliver such large coils. Transcatheter coil closure thus appears to be safe and effective for closing large patent ductus arteriosus in the elderly. PMID:11481843

  4. CLOSURE OF HLW TANKS FORMULATION FOR A COOLING COIL GROUT

    SciTech Connect

    Harbour, J; Vickie Williams, V; Erich Hansen, E

    2008-05-23

    The Tank Closure and Technology Development Groups are developing a strategy for closing the High Level Waste (HLW) tanks at the Savannah River Site (SRS). Two Type IV tanks, 17 and 20 in the F-Area Tank Farm, have been successfully filled with grout. Type IV tanks at SRS do not contain cooling coils; on the other hand, the majority of the tanks (Type I, II, III and IIIA) do contain cooling coils. The current concept for closing tanks equipped with cooling coils is to pump grout into the cooling coils to prevent pathways for infiltrating water after tank closure. This task addresses the use of grout to fill intact cooling coils present in most of the remaining HLW tanks on Site. The overall task was divided into two phases. Phase 1 focused on the development of a grout formulation (mix design) suitable for filling the HLW tank cooling coils. Phase 2 will be a large-scale demonstration of the filling of simulated cooling coils under field conditions using the cooling coil grout mix design recommended from Phase 1. This report summarizes the results of Phase 1, the development of the cooling coil grout formulation. A grout formulation is recommended for the full scale testing at Clemson Environmental Technology Laboratory (CETL) that is composed by mass of 90% Masterflow (MF) 816 (a commercially available cable grout) and 10% blast furnace slag, with a water to cementitious material (MF 816 + slag) ratio of 0.33. This formulation produces a grout that meets the fresh and cured grout requirements detailed in the Task Technical Plan (2). The grout showed excellent workability under continuous mixing with minimal change in rheology. An alternative formulation using 90% MF 1341 and 10% blast furnace slag with a water to cementitious material ratio of 0.29 is also acceptable and generates less heat per gram than the MF 816 plus slag mix. However this MF 1341 mix has a higher plastic viscosity than the MF 816 mix due to the presence of sand in the MF 1341 cable grout and a

  5. A Coiled-Coil Enabled Split-Luciferase Three-Hybrid System: Applied Toward Profiling Inhibitors of Protein Kinases

    PubMed Central

    Jester, Benjamin W.; Cox, Kurt J.; Gaj, Alicia; Shomin, Carolyn D.; Porter, Jason R.; Ghosh, Indraneel

    2010-01-01

    The 518 protein kinases encoded in the human genome are exquisitely regulated and their aberrant function(s) are often associated with human disease. Thus, in order to advance therapeutics and to probe signal transduction cascades there is considerable interest in the development of inhibitors that can selectively target protein kinases. However, identifying specific compounds against such a large array of protein kinases is difficult to routinely achieve utilizing traditional activity assays, where purified protein kinases are necessary. Toward a simple, rapid, and practical method for identifying specific inhibitors, we describe the development and application of a split-protein methodology utilizing a coiled-coil assisted three-hybrid system. In this approach, a protein kinase of interest is attached to the C-terminal fragment of split-firefly luciferase and the coiled-coil Fos, which is specific for the coiled-coil Jun, is attached to the N-terminal fragment. Upon addition of Jun conjugated to a pan-kinase inhibitor such as staurosporine, a three-hybrid complex is established with concomitant reassembly of the split-luciferase enzyme. An inhibitor can be potentially identified by the commensurate loss in split-luciferase activity by displacement of the modified staurosporine. We demonstrate that this new three-hybrid approach is potentially general by testing protein kinases from the different kinase families. To interrogate whether this method allows for screening inhibitors, we tested six different protein kinases against a library of 80 known protein kinase inhibitors. Finally, we demonstrate that this three-hybrid system can potentially provide a rapid method for structure/function analysis as well as aid in the identification of allosteric inhibitors. PMID:20669947

  6. Crystal Structure of Cytomegalovirus IE1 Protein Reveals Targeting of TRIM Family Member PML via Coiled-Coil Interactions

    PubMed Central

    Sevvana, Madhumati; Otto, Victoria; Schilling, Eva-Maria; Stump, Joachim D.; Müller, Regina; Reuter, Nina; Sticht, Heinrich; Muller, Yves A.; Stamminger, Thomas

    2014-01-01

    PML nuclear bodies (PML-NBs) are enigmatic structures of the cell nucleus that act as key mediators of intrinsic immunity against viral pathogens. PML itself is a member of the E3-ligase TRIM family of proteins that regulates a variety of innate immune signaling pathways. Consequently, viruses have evolved effector proteins to modify PML-NBs; however, little is known concerning structure-function relationships of viral antagonists. The herpesvirus human cytomegalovirus (HCMV) expresses the abundant immediate-early protein IE1 that colocalizes with PML-NBs and induces their dispersal, which correlates with the antagonization of NB-mediated intrinsic immunity. Here, we delineate the molecular basis for this antagonization by presenting the first crystal structure for the evolutionary conserved primate cytomegalovirus IE1 proteins. We show that IE1 consists of a globular core (IE1CORE) flanked by intrinsically disordered regions. The 2.3 Å crystal structure of IE1CORE displays an all α-helical, femur-shaped fold, which lacks overall fold similarity with known protein structures, but shares secondary structure features recently observed in the coiled-coil domain of TRIM proteins. Yeast two-hybrid and coimmunoprecipitation experiments demonstrate that IE1CORE binds efficiently to the TRIM family member PML, and is able to induce PML deSUMOylation. Intriguingly, this results in the release of NB-associated proteins into the nucleoplasm, but not of PML itself. Importantly, we show that PML deSUMOylation by IE1CORE is sufficient to antagonize PML-NB-instituted intrinsic immunity. Moreover, co-immunoprecipitation experiments demonstrate that IE1CORE binds via the coiled-coil domain to PML and also interacts with TRIM5α We propose that IE1CORE sequesters PML and possibly other TRIM family members via structural mimicry using an extended binding surface formed by the coiled-coil region. This mode of interaction might render the antagonizing activity less susceptible to

  7. Second coiled-coil domain of KCNQ channel controls current expression and subfamily specific heteromultimerization by salt bridge networks.

    PubMed

    Nakajo, Koichi; Kubo, Yoshihiro

    2008-06-15

    KCNQ channels carry the slowly activating, voltage-dependent M-current in excitable cells such as neurons. Although the KCNQ2 homomultimer can form a functional voltage-gated K(+) channel, heteromultimerization with KCNQ3 produces a > 10-fold increase in current amplitude. All KCNQ channels contain double coiled-coil domains (TCC1 and TCC2, or A-domain Head and Tail), of which TCC2 (A-domain Tail) is thought to be important for subunit recognition, channel assembly and surface expression. The mechanism by which TCC2 recognizes and associates with its partner is not fully understood, however. Our aim in the present study was to elucidate the recognition mechanism by examining the phenotypes of TCC2-deletion mutants, TCC2-swapped chimeras and point mutants. Electrophysiological analysis using Xenopus oocytes under two-electrode voltage clamp revealed that homotetrameric KCNQ3 TCC2 is a negative regulator of current expression in the absence of KCNQ2 TCC2. Recent structural analysis of KCNQ4 TCC2 revealed the presence of intercoil salt bridge networks. We therefore swapped the sign of the charged residues reportedly involved in the salt bridge formation and functionally confirmed that the intercoil salt bridge network is responsible for the subunit recognition between KCNQ2 and KCNQ3. Finally, we constructed TCC2-swapped KCNQ2/KCNQ3 mutants with KCNQ1 TCC2 or GCN4-pLI, a coiled-coil domain from an unrelated protein, and found that TCC2 is substitutable and even GCN4-pLI can work as a substitute for TCC2. Our present data provide some new insights into the role played by TCC2 during current expression, and also provide functional evidence of the importance of the intercoil salt bridge network for subunit recognition and coiled-coil formation, as is suggested by recent crystallographic data. PMID:18440995

  8. Oncogenic TPM3-ALK activation requires dimerization through the coiled-coil structure of TPM3

    SciTech Connect

    Amano, Yosuke; Ishikawa, Rie; Sakatani, Toshio; Ichinose, Junji; Sunohara, Mitsuhiro; Watanabe, Kousuke; Kage, Hidenori; Nakajima, Jun; Nagase, Takahide; Ohishi, Nobuya; Takai, Daiya

    2015-02-13

    Inflammatory myofibroblastic tumor (IMT) is a mesenchymal tumor that can arise from anywhere in the body. Anaplastic lymphoma kinase (ALK) gene rearrangements, most often resulting in the tropomyosin 3 (TPM3)-ALK fusion gene, are the main causes of IMT. However, the mechanism of malignant transformation in IMT has yet to be elucidated. The purpose of this study was to clarify the role of the TPM3 region in the transformation of IMT via TPM3-ALK. Lentivirus vectors containing a TPM3-ALK fusion gene lacking various lengths of TPM3 were constructed and expressed in HEK293T and NIH3T3 cell lines. Focus formation assay revealed loss of contact inhibition in NIH3T3 cells transfected with full-length TPM3-ALK, but not with ALK alone. Blue-native polyacrylamide gel electrophoresis (BN-PAGE) revealed that TPM3-ALK dimerization increased in proportion to the length of TPM3. Western blot showed phosphorylation of ALK, ERK1/2, and STAT3 in HEK293T cells transfected with TPM3-ALK. Thus, the coiled-coil structure of TPM3 contributes to the transforming ability of the TPM3-ALK fusion protein, and longer TPM3 region leads to higher dimer formation. - Highlights: • TPM3-ALK fusion protein dimerizes through the coiled-coil structure of TPM3. • Longer coiled-coil structure of TPM3 leads to higher TPM3-ALK dimer formation. • Presence of TPM3-ALK dimer leads to ALK, STAT3, and ERK1/2 phosphorylation. • Presence of TPM3-ALK leads to loss of contact inhibition. • BN-PAGE is a simple technique for visualizing oncogenic dimerization.

  9. Radio-frequency coil selection for MR imaging of the carotid vessel wall

    NASA Astrophysics Data System (ADS)

    Mat Isa, S.; Shuaib, I. L.; Bauk, S.

    2014-11-01

    This aim of this study was to identify the radiofrequency coil that will produce optimum image quality for scanning the carotid vessel wall using magnetic resonance imaging. A comparative cross-sectional study was conducted using 10 volunteers. Each volunteer was scanned three times using a 1.5T Signa HDxt machine equipped with one of three different coils: a neurovascular array (NV) coil, an 8-channel CTL spine array coil, and a 3-inch surface coil. A qualitative image quality rating was assigned to each image. The images were also evaluated by measuring the signal to noise ratio (SNR) using Osirix 4.2.3 software. The noise was estimated from the mean intensities of the region of interest in the background of the images and the signal was measured in the muscle adjacent to the vessel wall. The SNRs of the three coils were compared using one-way ANOVA, with 104 images used for the data analysis. The mean image quality scores for the NV head coil, CTL coil, and 3-inch coil were 3.4, 3.33, and 1.67, respectively. In addition, the SNRs differed significantly (p < 0.05). The mean SNR for the 3-inch coil was significantly higher (56.21 ± 25.06) than those for the NV head coil (27.34 ± 15.47) and CTL coil (21.77 ± 13.14). The Bonferroni post-hoc test revealed that there was no significant difference between the NV head coil and the CTL coil (p = 0.21). The optimum SNR value was 20-27. These results indicate that the NV head coil and CTL coil can be used to evaluate the carotid arterial wall with optimum image quality and higher resolution. These coil can deliver fast and robust data to image the carotid vessel wall in vivo.

  10. Formulation for a practical implementation of electromagnetic induction coils optimized using stream functions

    NASA Astrophysics Data System (ADS)

    Reed, Mark A.; Scott, Waymond R.

    2016-05-01

    Continuous-wave (CW) electromagnetic induction (EMI) systems used for subsurface sensing typically employ separate transmit and receive coils placed in close proximity. The closeness of the coils is desirable for both packaging and object pinpointing; however, the coils must have as little mutual coupling as possible. Otherwise, the signal from the transmit coil will couple into the receive coil, making target detection difficult or impossible. Additionally, mineralized soil can be a significant problem when attempting to detect small amounts of metal because the soil effectively couples the transmit and receive coils. Optimization of wire coils to improve their performance is difficult but can be made possible through a stream-function representation and the use of partially convex forms. Examples of such methods have been presented previously, but these methods did not account for certain practical issues with coil implementation. In this paper, the power constraint introduced into the optimization routine is modified so that it does not penalize areas of high current. It does this by representing the coils as plates carrying surface currents and adjusting the sheet resistance to be inversely proportional to the current, which is a good approximation for a wire-wound coil. Example coils are then optimized for minimum mutual coupling, maximum sensitivity, and minimum soil response at a given height with both the earlier, constant sheet resistance and the new representation. The two sets of coils are compared both to each other and other common coil types to show the method's viability.

  11. Mid-Range Coil Array for Magnetic Resonance Imaging of Small Animals

    NASA Astrophysics Data System (ADS)

    Solis, S. E.; Tomasi, D.; Rodríguez, A. O.

    2008-08-01

    The vast majority of articles on MRI RF coils over the past two decades have focused on large coils, where sample losses dominate, or on micro-coils, where sample and capacitor losses are negligible. Few have addressed the mid-range coils, seen in the majority of small-animal applications, where all the sources of loss are important, for example, mouse brain and body coils from 125 to 750 MHz. We developed a four-saddle coil array for magnetic resonance imaging of small animals. The saddle coil elements in the array were evenly distributed to cover the rat's head. The coil array was tuned to the resonant frequency of 170 MHz. Due to the close proximity of the coil elements, it was necessary to decouple the coil array using nonmagnetic trimmers and, it was operated in the transceiver mode and quadrature-driven. To test the coil array performance at high field, phantom images were acquired with our saddle coil array and standard pulse sequences on a research-dedicated 4 Tesla scanner. Ex vivo brain images of a rat were also acquired, and proved the feasibility of the scaled version of a saddle coil array and, its compatibility with standard pulse sequences when used in a high field magnetic resonance imager.

  12. Method and apparatus for magnetic resonance imaging and spectroscopy using microstrip transmission line coils

    DOEpatents

    Zhang, Xiaoliang; Ugurbil, Kamil; Chen, Wei

    2006-04-04

    Apparatus and method for MRI imaging using a coil constructed of microstrip transmission line (MTL coil) are disclosed. In one method, a target is positioned to be imaged within the field of a main magnetic field of a magnet resonance imaging (MRI) system, a MTL coil is positioned proximate the target, and a MRI image is obtained using the main magnet and the MTL coil. In another embodiment, the MRI coil is used for spectroscopy. MRI imaging and spectroscopy coils are formed using microstrip transmission line. These MTL coils have the advantageous property of good performance while occupying a relatively small space, thus allowing MTL coils to be used inside restricted areas more easily than some other prior art coils. In addition, the MTL coils are relatively simple to construct of inexpensive components and thus relatively inexpensive compared to other designs. Further, the MTL coils of the present invention can be readily formed in a wide variety of coil configurations, and used in a wide variety of ways. Further, while the MTL coils of the present invention work well at high field strengths and frequencies, they also work at low frequencies and in low field strengths as well.

  13. A multi-slot surface coil for MRI of dual-rat imaging at 4T

    SciTech Connect

    Solis, S.E.; Tomasi, D.; Solis, S.E.; Wang, R.; Tomasi, D.; Rodriguez, A.O.

    2011-07-01

    A slotted surface coil inspired by the hole-and-slot cavity magnetron was developed for magnetic resonance imaging of obese rats at 4 T. Full-wave analysis of the magnetic field was carried out at 170 MHz for both the slotted and circular-shaped coils. The noise figure values of two coils were investigated via the numerical calculation of the quality factors. Fat simulated phantoms to mimic overweight rats were included in the analysis with weights ranging from 300 to 900 g. The noise figures were 1.2 dB for the slotted coil and 2.4 dB for the circular coil when loaded with 600 g of simulated phantom. A slotted surface coil with eight circular slots and a circular coil with similar dimensions were built and operated in the transceiver mode, and their performances were experimentally compared. The imaging tests in phantoms demonstrated that the slotted surface coil has a deeper RF-sensitivity and better field uniformity than the single-loop RF-coil. High quality images of two overweight Zucker rats were acquired simultaneously with the slotted surface coil using standard spin-echo pulse sequences. Experimental results showed that the slotted surface coil outperformed the circular coil for imaging considerably overweight rats. Thus, the slotted surface coil can be a good tool for MRI experiments in rats on a human whole-body 4 T scanner.

  14. A multi-slot surface coil for MRI of dual-rat imaging at 4 T

    NASA Astrophysics Data System (ADS)

    Solis, S. E.; Wang, R.; Tomasi, D.; Rodriguez, A. O.

    2011-06-01

    A slotted surface coil inspired by the hole-and-slot cavity magnetron was developed for magnetic resonance imaging of obese rats at 4 T. Full-wave analysis of the magnetic field was carried out at 170 MHz for both the slotted and circular-shaped coils. The noise figure values of two coils were investigated via the numerical calculation of the quality factors. Fat simulated phantoms to mimic overweight rats were included in the analysis with weights ranging from 300 to 900 g. The noise figures were 1.2 dB for the slotted coil and 2.4 dB for the circular coil when loaded with 600 g of simulated phantom. A slotted surface coil with eight circular slots and a circular coil with similar dimensions were built and operated in the transceiver mode, and their performances were experimentally compared. The imaging tests in phantoms demonstrated that the slotted surface coil has a deeper RF-sensitivity and better field uniformity than the single-loop RF-coil. High quality images of two overweight Zucker rats were acquired simultaneously with the slotted surface coil using standard spin-echo pulse sequences. Experimental results showed that the slotted surface coil outperformed the circular coil for imaging considerably overweight rats. Thus, the slotted surface coil can be a good tool for MRI experiments in rats on a human whole-body 4 T scanner.

  15. Development of Computational Models for Coiling Process with the Belt Wrapper

    NASA Astrophysics Data System (ADS)

    Park, Yonghui; Park, Hyunchul

    2016-07-01

    This study introduces coiling mechanism with the belt wrapper to understand a force equilibrium for successful coiling. By establishing a finite element (FE) model, strips were coiled 2 to 3 rotations by the belt wrapper on the sleeve without coiling tension T, then T was applied to the opposite side of the strips near the pinch roller, and the belt wrapper was removed from the strip coil at the same time. Additionally, analytical model corresponding to FE model was defined by thick and thin cylinder theorems to quantize coiling mechanisms. Especially elasticity of the belt wrapper E [N/m2], coiling tension T [N/m2], and friction coefficient μ were checked on how these variables affect each other, were converted into pressure P [N/m2], and P were used to calculate when the strip coil come untied. For instance, the strip coil came untied when E was lower than 1 × 109 N/m2 corresponding to ( {Pressure on outmost of the belt wrapper} P_{o,belt} / {Pressure on innermost of the sleeve} P_{i,sleeve} = 0.877 ) . Lastly, radial stress on the outmost of the sleeve σ r,o,sleeve [N/m2] according to E were compared to the previous coiling method with the grooved joint to see how these methods are different. Based on these results, this paper suggests coiling criteria to avoid coiling failure of slip of the strip coil.

  16. Mid-Range Coil Array for Magnetic Resonance Imaging of Small Animals

    SciTech Connect

    Solis, S. E.; Tomasi, D.; Rodriguez, A. O.

    2008-08-11

    The vast majority of articles on MRI RF coils over the past two decades have focused on large coils, where sample losses dominate, or on micro-coils, where sample and capacitor losses are negligible. Few have addressed the mid-range coils, seen in the majority of small-animal applications, where all the sources of loss are important, for example, mouse brain and body coils from 125 to 750 MHz. We developed a four-saddle coil array for magnetic resonance imaging of small animals. The saddle coil elements in the array were evenly distributed to cover the rat's head. The coil array was tuned to the resonant frequency of 170 MHz. Due to the close proximity of the coil elements, it was necessary to decouple the coil array using nonmagnetic trimmers and, it was operated in the transceiver mode and quadrature-driven. To test the coil array performance at high field, phantom images were acquired with our saddle coil array and standard pulse sequences on a research-dedicated 4 Tesla scanner. Ex vivo brain images of a rat were also acquired, and proved the feasibility of the scaled version of a saddle coil array and, its compatibility with standard pulse sequences when used in a high field magnetic resonance imager.

  17. Superconducting coil and method of stress management in a superconducting coil

    DOEpatents

    McIntyre, Peter M.; Shen, Weijun; Diaczenko, Nick; Gross, Dan A.

    1999-01-01

    A superconducting coil (12) having a plurality of superconducting layers (18) is provided. Each superconducting layer (18) may have at least one superconducting element (20) which produces an operational load. An outer support structure (24) may be disposed outwardly from the plurality of layers (18). A load transfer system (22) may be coupled between at least one of the superconducting elements (20) and the outer support structure (24). The load transfer system (22) may include a support matrix structure (30) operable to transfer the operational load from the superconducting element (20) directly to the outer support structure (24). A shear release layer (40) may be disposed, in part, between the superconducting element (20) and the support matrix structure (30) for relieving a shear stress between the superconducting element (20) and the support matrix structure (30). A compliant layer (42) may also be disposed, in part, between the superconducting element (20) and the support matrix structure (30) for relieving a compressive stress on the superconducting element (20).

  18. Design of Nb3Sn Coils for LARP Long Magnets

    SciTech Connect

    Ferracin, Paolo; Ambrosio, G.; Andreev, N.; Anerella, M.; Barzi, E.; Bossert, R.; Ferracin, P.; Caspi, S.; Lietzke, A. F.

    2007-06-01

    The LHC Accelerator Research Program (LARP) has a primary goal to develop, assemble, and test full size Nb{sub 3}Sn quadrupole magnet models for a luminosity upgrade of the Large Hadron Collider (LHC). A major milestone in this development is to assemble and test, by the end of 2009, two 4 m-long quadrupole cold masses, which will be the first Nb{sub 3}Sn accelerator magnet models approaching the length of real accelerator magnets. The design is based on the LARP Technological Quadrupoles (TQ), under development at FNAL and LBNL, with gradient higher than 200 T/m and aperture of 90 mm. The mechanical design will be chosen between two designs presently explored for the TQs: traditional collars and Al-shell based design (preloaded by bladders and keys). The fabrication of the first long quadrupole model is expected to start in the last quarter of 2007. Meanwhile the fabrication of 4 m-long racetrack coils started this year at BNL. These coils will be tested in an Al-shell based supporting structure developed at LBNL. Several challenges have to be addressed for the successful fabrication of long Nb{sub 3}Sn coils. This paper presents these challenges with comments and solutions adopted or under study for these magnets. The coil design of these magnets, including conductor and insulation features, and quench protection studies are also presented.

  19. Differential-Coil Eddy-Current Material Sorter

    NASA Technical Reports Server (NTRS)

    Nummelin, J.; Buckley, D.

    1985-01-01

    Small metal or other electrically conductive parts of same shape but different composition quickly sorted with differential-coil eddy-current sorter. Developed to distinguish between turbine blades of different alloys, hardnesses, and residual stress, sorter generally applicable to parts of simple and complex shape.

  20. Microengineered needle micro-coils for magnetic resonance spectroscopy

    NASA Astrophysics Data System (ADS)

    Syms, R. R. A.; Ahmad, M. M.; Young, I. R.; Gilderdale, D. J.; Collins, D. J.

    2006-12-01

    A process for batch fabrication of low-cost needle-shaped micro-coils for magnetic resonance (MR) spectroscopy is demonstrated. The conductors are embedded inside a cross-section designed to avoid the signal cancellation effects that can occur with completely immersed detectors. Simple models are developed for the sensitivity of an immersed coil and for the electrical performance of coils on silicon substrates. Conductors are fabricated on oxidized Si by electroplating metals inside a deep photoresist mould, and then capped with a thick layer of plastic. Through-wafer deep reactive ion etching is used to define needle shapes. At 63.8 MHz frequency, Q-factors obtained on Si are comparable to those on glass, and resonators based on single-turn coils have Q-factors of ap14. Total immersion 1H MR imaging and spectroscopy are demonstrated in a 1.5 T magnetic field using tomato fruits. Q-factors are raised at higher frequencies (to >30 at 255 MHz) using thick polymer isolation, and hybrid integration of additional circuitry is demonstrated.