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Sample records for fork projects thompson

  1. Wildlife Impact Assessment and Summary of Previous Mitigation Related to Hydroelectric Projects in Montana, Phase I, Volume Two (A), Clark Fork Projects, Thompson Falls Dam, Operator, Montana Power Company.

    SciTech Connect

    Wood, Marilyn

    1984-03-27

    The Thompson Falls Dam inundated approximately 347 acres of wildlife habitat that likely included conifer forests, deciduous bottoms, mixed conifer-deciduous forests and grassland/hay meadows. Additionally, at least one island, and several gravel bars were inundated when the river was transformed into a reservoir. The loss of riparian and riverine habitat adversely affected the diverse wildlife community inhabiting the lower Clark Fork River area. Quantitative loss estimates were determined for selected target species based on best available information. The loss estimates were based on inundation of the habitat capable of supporting the target species. Whenever possible, loss estimates bounds were developed by determining ranges of impacts based on density estimates and/or acreage loss estimates. Of the twelve target species or species groups, nine were assessed as having net negative impacts. 86 refs., 2 figs., 5 tabs.

  2. Final 2014 Remedial Action Report Project Chariot, Cape Thompson, Alaska

    SciTech Connect

    None, None

    2015-03-01

    This report was prepared to document remedial action (RA) work performed at the former Project Chariot site located near Cape Thompson, Alaska during 2014. The work was managed by the U.S. Army Corps of Engineers (USACE) Alaska District for the U.S. Department of Energy (DOE) Office of Legacy Management (LM). Due to the short field season and the tight barge schedule, all field work was conducted at the site July 6 through September 12, 2014. Excavation activities occurred between July 16 and August 26, 2014. A temporary field camp was constructed at the site prior to excavation activities to accommodate the workers at the remote, uninhabited location. A total of 785.6 tons of petroleum, oil, and lubricants (POL)-contaminated soil was excavated from four former drill sites associated with test holes installed circa 1960. Diesel was used in the drilling process during test hole installations and resulted in impacts to surface and subsurface soils at four of the five sites (no contamination was identified at Test Hole Able). Historic information is not definitive as to the usage for Test Hole X-1; it may have actually been a dump site and not a drill site. In addition to the contaminated soil, the steel test hole casings were decommissioned and associated debris was removed as part of the remedial effort.

  3. 78 FR 13660 - Middle Fork American River Project; Notice of Availability of the Final Environmental Impact...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Energy Regulatory Commission Middle Fork American River Project; Notice of Availability of the Final Environmental Impact Statement for the Middle Fork American River Hydrolectric Project In accordance with the... Projects has reviewed the application for license for the Middle Fork American River Hydroelectric...

  4. Wildlife and Wildlife Habitat Mitigation Plan for the Thompson Falls Hydroelectric Project, Final Report.

    SciTech Connect

    Bissell, Gael; Wood, Marilyn

    1985-08-01

    This document presents a preliminary mitigation and enhancement plan for the Thompson Falls hydroelectric project. It discusses options available to provide wildlife protection, mitigation and enhancement in accordance with the Pacific Northwest Electric Power Planning and Conservation Act of 1980 (P.L. 96-501). The options focus on mitigation for wildlife and wildlife habitat losses attributable to the construction of the hydroelectric project. These losses were previously estimated from the best available information concerning the degree of negative and positive impacts to target wildlife species (Wood and Olsen 1984). Criteria by which the mitigation alternatives were evaluated were the same as those used to assess the impacts identified in the Phase I document (Wood and Olsen 1984). They were also evaluated according to feasibility and cost effectiveness. This document specifically focuses on mitigation for target species which were identified during Phase I (Wood and Olsen 1984). It was assumed mitigation and enhancement for the many other target wildlife species impacted by the hydroelectric developments will occur as secondary benefits. The recommended mitigation plan includes two recommended mitigation projects: (1) development of wildlife protection and enhancement plans for MPC lands and (2) strategies to protect several large islands upstream of the Thompson Falls reservoir. If implemented, these projects would provide satisfactory mitigation for wildlife losses associated with the Thompson Falls hydroelectric project. The intent of the mitigation plan is to recommend wildlife management objectives and guidelines. The specific techniques, plans, methods and agreements would be developed is part of the implementation phase.

  5. Oak Grove Fork Habitat Improvement Project, 1988 Annual Report.

    SciTech Connect

    Bettin, Scott

    1989-04-01

    The Lower Oak Grove Fork of the Clackamas River is a fifth-order tributary of the Clackamas River drainage supporting depressed runs of coho and chinook salmon, and summer and winter steelhead. Habitat condition rating for the Lower Oak Grove is good, but smelt production estimates are below the average for Clackamas River tributaries. Limiting factors in the 3.8 miles of the Lower Oak Grove supporting anadromous fish include an overall lack of quality spawning and rearing habitat. Beginning in 1986. measures to improve fish habitat in the Lower Oak Grove were developed in coordination with the Oregon Department of Fish and Wildlife (ODF&W) and Portland General Electric (PGE) fisheries biologists. Prior to 1986, no measures had been applied to the stream to mitigate for PGE's storage and regulation of flows in the Oak Grove Fork (Timothy Lake, Harriet Lake). Catchable rainbow trout are stocked by ODF&W two or three times a year during the trout fishing season in the lowermost portion of the Oak Grove Fork near two Forest Service campgrounds (Ripplebrook and Rainbow). The 1987 field season marked the third year of efforts to improve fish habitat of the Lower Oak Grove Fork and restore anadromous fish production. The efforts included the development of an implementation plan for habitat improvement activities in the Lower Oak Grove Fork. post-project monitoring. and maintenance of the 1986 improvement structures. No new structures were constructed or placed in 1987. Fiscal year 1988 brought a multitude of changes which delayed implementation of plans developed in 1987. The most prominent change was the withdrawal of the proposed Spotted Owl Habitat Area (SOHA) which overlapped the Oak Grove project implementation area. Another was the change in the Forest Service biologist responsible for implementation and design of this project.

  6. Penstock inspection and safety assessment program: Big Thompson Powerplant Colorado-Big Thompson Project, Colorado. Review report, Great Plains region

    SciTech Connect

    1997-06-01

    On February 26-28, 1997, and June 16-17, 1997, the Big Thompson penstock was inspected. During the exam the following tasks were performed: (1) The condition of the exposed exterior coating and the interior lining of the penstock was inspected; (2) The wall thickness of the steel penstock was determined by ultrasonic testing; (3) Appurtenances where the penstock and piping connected to the penstock change in diameter or where the water flow changes in direction were inspected; (4) Joints, piping connections, and various associated operating equipment (gates, valves, etc.) were inspected; (5) Load rejection tests, unwatered governor testing, and emergency gate closure tests were performed.

  7. 77 FR 45597 - Middle Fork American River Project; Notice of Availability of the Draft Environmental Impact...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-01

    ... Energy Regulatory Commission Middle Fork American River Project; Notice of Availability of the Draft Environmental Impact Statement for the Middle Fork American River Hydrolectric Project and Intention To Hold... CFR part 380 ), the Office of Energy Projects has reviewed the application for license for the...

  8. LAKE FORK

    EPA Science Inventory

    The Lake Fork of the Arkansas River Watershed has been adversely affected through mining, water diversion and storage projects, grazing, logging, and other human influences over the past 120 years. It is the goals of the LFWWG to improve the health of Lake fork by addressing th...

  9. South Fork Snake River/Palisades Wildlife Mitigation Project: Environmental assessment

    SciTech Connect

    1995-09-01

    BPA proposes to fund the implementation of the South Fork Snake River Programmatic Management Plan to compensate for losses of wildlife and wildlife habitat due to hydroelectric development at Palisades Dam. The Idaho Department of Fish and Game drafted the plan, which was completed in May 1993. This plan recommends land and conservation easement acquisition and wildlife habitat enhancement measures. These measures would be implemented on selected lands along the South Fork of the Snake River between Palisades Dam and the confluence with the Henry`s Fork, and on portions of the Henry`s Fork located in Bonneville, Madison, and Jefferson Counties, Idaho. BPA has prepared an Environmental Assessment evaluating the proposed project. The EA also incorporates by reference the analyses in the South Fork Snake River Activity/Operations Plan and EA prepared jointly in 1991 by the Bureau of Land Management and the Forest Service. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an Environmental Impact Statement (EIS) is not required and BPA is issuing this FONSI.

  10. 77 FR 47058 - Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft Environmental Impact Statement; Public Meetings a. Date and Time of Meetings: Tuesday, August 28, 2012, from 9:00 a.m. to...

  11. 3. SOUTH FORK OF THE TULE RIVER MIDDLE FORK BRANCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. SOUTH FORK OF THE TULE RIVER MIDDLE FORK BRANCH FLUME AT THE NORTH FORK OF THE TULE RIVER MIDDLE FORK CROSSING SHOWING ORIGINAL DIMENSIONAL STONE PIER ON WEST BANK AT PHOTO CENTER, AND REMAINS OF ORIGINAL EAST BANK DIMENSIONAL STONE PIER AT PHOTO LEFT BELOW NEW (ca. 1931) EAST BANK PIER. VIEW TO SOUTHEAST. - Tule River Hydroelectric Project, Water Conveyance System, Middle Fork Tule River, Springville, Tulare County, CA

  12. Milton (Milt) O. Thompson

    NASA Technical Reports Server (NTRS)

    1960-01-01

    Milton O. Thompson was hired as an aeronautical research scientist at the National Advisory Committee for Aeronautics' High-Speed Flight Station, Edwards, California, on March 19, 1956, becoming a research pilot in January 1958. During his 37-year career at what became the National Aeronautics and Space Administration's Dryden Flight Research Center in 1976, Milt managed several research programs and flew many research flights. One of Milt's first research projects, after he became a research pilot, was a program to investigate the feasibility of obtaining substantial amounts of laminar flow on an airfoil at supersonic speeds. The testbed aircraft was an F-104 with one wing covered with a fiberglass glove that served as the test section for the experiment. Next was the Air Launched Sounding Rocket (ALSOR) research program using an F-104 with a rocket launcher installed on it. The intent of the program was to release a balloon from an air launched rocket at over 1,000,000 feet altitude (approximately 190 miles) and then measure its rate of descent to determine air density. In 1959, Thompson was assigned to the Boeing X-20 Dyna-Soar program as a pilot-consultant. The X-20 program was scheduled to launch a human into Earth orbit and recover with a horizontal ground landing. The program was canceled before construction of the vehicle began. Milt became interested in the Rogallo-wing concept, 'Parawing', for spacecraft reentry. The best way to acquire experience, of course, was by building and flying a Paraglider Research Vehicle (Paresev). After ground tows to demonstrate controllability with Milt in the cockpit, he made the first flight aloft on March 12, 1962. On August 16, 1963 Thompson became the first person to fly a lifting body, the lightweight M2-F1. The plywood and steel-tubing prototype was flown as a glider after releasing from an R4D tow plane. He flew it a total of 47 times, and also made the first five flights of the all-metal M2-F2 lifting body

  13. Project Management Done Here: Lois Langer--Thompson Hennepin County Library, MN

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    How do you simultaneously renovate a grocery store into a state-of-the-art resource library, supervise the daily operations of five libraries, lead a team in reimagining reference service, and plan your wedding? Ask Lois Langer, coordinating librarian of Hennepin County Library. Her answer: "Project management--you can do anything, professional…

  14. Interview with Sandra Thompson.

    ERIC Educational Resources Information Center

    Huang, Chiung-chih

    1994-01-01

    Presents an interview of Sandra Thompson on various topics relating to the Chinese language. The interview touches on conversational data on Chinese, the lack of morphological complexity in Mandarin Chinese, and the development of Chinese functionalism. (12 references) (CK)

  15. North Fork John Day Dredge Tailings Restoration Project Final Report 1997-2002.

    SciTech Connect

    Sanchez, John A.

    2002-12-01

    The USDA Forest Service (USFS) and the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) worked together to rehabilitate 2.1 miles of Clear Creek floodplain, a tributary of the North Fork John Day River Basin. Dredge tailing were deposited from mining operations on Clear Creek's floodplain from the 1930's to the 1950's. These tailing confined the stream channel and degraded the floodplain. The work was completed by moving dredge tailing piles adjacent to the Clear Creek channel, using track-mounted excavators and dump trucks. A caterpillar tractor was used to contour the material placed outside the immediate floodplain, blending it into the hillside. The restored floodplain was very near channel bankfull level following excavation and contoured to accept future flood flows. Monitoring was initiated through pre and post-project photo points and cross-section measurements. Work was completed in two efforts. In 1997 and 1998 floodplain restoration was adjacent to the reconstruction of Road 13 from the junction with Road 10 from Clear Creek River Mile 1.9 to 3.1 for a distance of 1.2 miles. In 1999 the Environmental Assessment for Lower Clear Creek--Granite Creek Floodplain Restoration Project was completed for work proposed on Clear Creek from the mouth up to River mile 1.9 and the Granite Creek floodplain from River miles 5.9 to 7.7. Restoration proposed in the 1999 Environmental Assessment is the subject of this report.

  16. Maxine M. Thompson - Dedication

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This manuscript summarizes the research career of Dr. Maxine M. Thompson, world renown horticulturist, plant breeder, and plant explorer. She became the first women professor at the Oregon State University, Deparment of Horticulture. She studied Rubus cytology and genetics and floral development in ...

  17. South Fork Tolt River Hydroelectric Project : Adopted Portions of a 1987 Federal Energy Regulatory Commission`s Final Environmental Impact Statement.

    SciTech Connect

    United States. Bonneville Power Administration.

    1992-07-01

    The South Fork Tolt River Hydroelectric Project that world produce 6.55 average megawatts of firm energy per year and would be sited in the Snohomish River Basin, Washington, was evaluated by the Federal Energy Regulatory commission (FERC) along with six other proposed projects for environmental effects and economic feasibility Based on its economic analysis and environmental evaluation of the project, the FERC staff found that the South Fork Tolt River Project would be economically feasible and would result in insignificant Impacts if sedimentation issues could be resolved. Upon review, the BPA is adopting portions of the 1987 FERC FEIS that concern the South Fork Tolt River Hydroelectric Project and updating specific sections in an Attachment.

  18. Wildlife and Wildlife Habitat Loss Assessment at Dexter Dam and Reservoir Project, Middle Fork Willamette River, Oregon, 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Dexter Dam and Reservoir Project on the Middle Fork Willamette River, Oregon, to determine losses or gains resulting from the development and operation of the project. Preconstruction, post-construction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1944, 1956, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Fifteen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Dexter Project extensively altered or affected 4662 acres of land and river in the Middle Fork Willamette River drainage. Impacts to wildlife centered around the loss of 445 acres of riparian habitat. Impacts resulting from the Dexter Project included the loss of year-round habitat for black-tailed deer, red fox, mink, beaver, western gray squirrel, ruffed grouse, ring-necked pheasant, California quail, wood duck and nongame species. Bald eagle, osprey, and greater scaup were benefitted by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Dexter Project. Losses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  19. James R. Thompson

    NASA Technical Reports Server (NTRS)

    1986-01-01

    James R. Thompon served as director of the Marshall Space Flight Center from September 29, 1986 until July 6, 1989, when he was appointed as NASA Deputy Administrator. Prior to his tenure as Marshall's Director, Thompson served from March to June 1986 as the vice-chairman of the NASA task force investigating the cause of the Space Shuttle Challenger accident. He was credited with playing a significant role in returning the Space Shuttle to flight following the Challenger disaster.

  20. Bruce Thompson - International leader

    NASA Astrophysics Data System (ADS)

    Cawley, Peter

    2012-05-01

    Bruce Thompson was not only a world leading scientist and engineer on the basis of his personal research, but he also led international efforts in QNDE. He was enormously helpful to the NDE community in the UK during the formation of the UK Centre, RCNDE, and the World Federation of NDE Centers. He traveled widely and was always interested in different national cultures and political systems. A brief appreciation of his influence on the international stage is given.

  1. Arsenic Removal from Drinking Water by Coagulation/Filtration U.S. EPA Demonstration Project at City of Three Forks, MT, Final Performance Evaluation Report

    EPA Science Inventory

    This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at the City of Three Forks, MT facility. The objectives of the project were to evaluate: 1) the effectiveness of Kinetico’s FM-248-A...

  2. Best management practices plan for the Lower East Fork Poplar Creek remedial action project, Oak Ridge, Tennessee

    SciTech Connect

    1996-08-01

    The U.S. Department of Energy (DOE) has three major operating facilities on the DOE Oak Ridge Reservation (ORR) in Oak Ridge, Tennessee: the Oak Ridge Y-12 Plant, the K-25 Site, and the Oak Ridge National Laboratory (ORNL) managed by Lockheed Martin Environmental Research Corporation. All facilities are managed by Lockheed Martin Energy Systems, Incorporated (Energy Systems) for the DOE. The Y-12 Plant is adjacent to the city of Oak Ridge and is also upstream from Oak Ridge along East Fork Poplar Creek. The portion of the creek downstream from the Y-12 Plant is Lower East Fork Poplar Creek (LEFPC). This project will remove mercury-contaminated soils from the LEFPC floodplain, transport the soils to Industrial Landfill V (ILF-V), and restore any affected areas. This project contains areas that were designated in 1989 as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) site. The site includes DOE property and portions of commercial, residential, agricultural, and miscellaneous areas within the city of Oak Ridge.

  3. Wildlife and Wildlife Habitat Loss Assessment at Green Peter-Foster Project; Middle Fork Santiam River, Oregon, 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1986-02-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Green Peter-Foster Dam and Reservoir Project on the Middle Fork Santiam River, Oregon, to determine losses or gains resulting from the development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types at the project site were mapped based on aerial photographs from 1955, 1972, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Eleven wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Green Peter-Foster Project extensively altered or affected 7873 acres of land and river in the Santiam River drainage. Impacts to wildlife centered around the loss of 1429 acres of grass-forb vegetation, 768 acres of shrubland, and 717 acres of open conifer forest cover types. Impacts resulting from the Green Peter-Foster Project included the loss of critical winter range for black-tailed deer and Roosevelt elk, and the loss of year-round habitat for deer, upland game birds, river otter, beaver, pileated woodpecker, and many other wildlife species. Bald eagle and osprey were benefited by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Green Peter-Foster Project. Losses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  4. Confederated Tribes of the Umatilla Indian Reservation North Fork John Day River Basin Anadromous Fish Enhancement Project, Annual Report for FY 2000.

    SciTech Connect

    Macy, Tom L.; James, Gary A.

    2003-03-01

    The CTUIR North Fork John Day River Basin Anadromous Enhancement Project (NFJDAFEP) identified and prioritized stream reaches in The North Fork John day River basin for habitat improvements during the 2000 project period. Public out reach was emphasized during this first year of the project. We presented multiple funding and enhancement options to landowners. We concentrated on natural recovery methods, riparian fencing and off-stream livestock water developments. Under this BPA contract four riparian easements were signed protecting almost 5 miles of tributary streams. There are nine offstream water developments associated with these easements. Some landowners chose to participate in other programs based on Tribal outreach efforts. Two landowners chose NRCS programs for enhancement and one chose OWEB as a funding source. Two landowners implemented there own enhancement measures protecting 3 miles of stream. Cooperation between the NRCS/FSA/SWCDs and the Tribe to create joint projects and develop alternative funding scenarios for riparian enhancement was a major effort. The Tribe also worked with the North Fork John Day Watershed Council, USFS and ODFW to coordinate projects and support similar projects throughout the John Day Basin. We provided input to the John Day Summary prepared for the NWPPC by ODFW. The Tribe worked with the Umatilla National Forest on the Clear Creek Dredgetailings Rehabilitation project and coordinated regularly with USFS Fisheries, Hydrology and Range staff.

  5. Confederated Tribes of the Umatilla Indian Reservation North Fork John Day River Basin Anadromous Fish Enhancement Project, Annual Report for FY 2001.

    SciTech Connect

    Macy, Tom L.; James, Gary A.

    2003-03-01

    The CTUIR North Fork John Day River Basin Anadromous Enhancement Project (NFJDAFEP) identified and prioritized stream reaches in The North Fork John day River basin for habitat improvements during the 2000 project period. Public outreach was emphasized during this first year of the project. During the past year we concentrated on satisfying landowner needs, providing cost share alternatives, providing joint projects and starting implementation. We presented multiple funding and enhancement options to landowners. We concentrated on natural recovery methods, riparian fencing and offstream livestock water developments. Under this BPA contract four riparian easements have been signed protecting almost 5 miles of tributary streams. There are nine offstream water developments associated with these easements. Some landowners chose to participate in other programs based on Tribal outreach efforts. Some landowners chose NRCS programs for enhancement and others chose OWEB as a funding source. The exact amount of stream protection due to other funding sources probably exceeds that by BPA, however most would not have entered any program without initial Tribal outreach. Cooperation between the NRCS/FSA/SWCDs and the Tribe to create joint projects and develop alternative funding scenarios for riparian enhancement was a major effort. The Tribe also worked with the North Fork John Day Watershed Council, USFS and ODFW to coordinate projects and support similar projects throughout the John Day Basin.

  6. Post construction report for Lower East Fork Poplar Creek Project, Phase 1, Oak Ridge, Tennessee

    SciTech Connect

    1996-11-01

    This Phase 1 Remedial Action (RA) effort was conducted in accordance with the Record of Decision (ROD) for Lower East Fork Poplar Creek (LEFPC) as a Comprehensive Environmental Response, Compensation, and Liability Act action. The LEFPC, Phase 1 RA removed approximately 5,560 yd{sup 3} of mercury-contaminated soils, {ge} 400 ppm, from selected portions of the National Oceanographic and Atmospheric Administration (NOAA) site LEFPC floodplain from July 8, 1996--September 14, 1996. During excavation activities, pockets of elevated radiologically contaminated soils (greater than 35 pCi/g) were located by the continuous monitoring of the excavation areas and contaminated soils with radiological monitoring instruments. Through characterization sampling it has been determined that {approximately} 90 yd{sup 3} are less than 35 pCi/g uranium contaminated and will be transported to the Y-12 Landfill V for disposal and the remaining {approximately}40 yd{sup 3} do not meet the WAC for radiological constituents included in the Special Waste Permit for Landfill V. The radiologically contaminated soil will be placed in 21st Century containers for storage at the K-25 site.

  7. Design criteria applied for the Lower Pressure Tunnel of the North Fork Stanislaus River Hydroelectric Project in California

    NASA Astrophysics Data System (ADS)

    Schleiss, A.

    1988-07-01

    The application of various criteria and certain new approaches to design is illustrated by the example of the Lower Collierville Pressure Tunnel of the North Fork Stanislaus River Hydropower Project in California. With a maximum internal water pressure of 72 bar, Lower Collierville Tunnel will be, when commissioned in 1989, the highest stressed pressure tunnel in the world not situated in granitic rocks. The geological conditions and the results of the geotechnical investigations are described briefly. For the steel-lined portion of the tunnel, the approach for determining the bearing capacity of the rock mass and the load sharing between steel and rock is discussed. The required length of steel liner was determined on the basis of rock mechanical (hydraulic jacking) and rock hydraulic (seepage losses and extension of saturated zone due to seepage) criteria. The use of a new theory allows the effects of mechanical-hydraulic interaction to be taken into account. Finally the methods of estimating the expected water losses and the sealing effect of the consolidation grouting are described.

  8. 15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO SETTLING BASIN, SHOWING RIGHT FORK WITH GATE IN PLACE AND A FEW NEEDLES IN PLACE - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

  9. EPA Region 10 Climate Change and TMDL Pilot Project - South Fork Nooksack River, Washington

    EPA Science Inventory

    The U.S. Environmental Protection Agency (EPA) Region 10 and EPA’s Office of Research and Development (ORD) and Office of Water (OW) have launched a pilot research project to consider how projected climate change impacts could be incorporated into a TMDL and influence restoration...

  10. 76 FR 46721 - Salmon-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... the potential for large-scale, high-intensity wildfires that threaten human life, property, and... project would use the existing transportation system except for the construction of approximately 14 miles... landscape fire resilience through prescribe burning, fish habitat and passage restoration are...

  11. 1976 Big Thompson flood, Colorado

    USGS Publications Warehouse

    Jarrett, R. D., (compiler); Vandas, S.J.

    2006-01-01

    In the early evening of July 31, 1976, a large stationary thunderstorm released as much as 7.5 inches of rainfall in about an hour (about 12 inches in a few hours) in the upper reaches of the Big Thompson River drainage. This large amount of rainfall in such a short period of time produced a flash flood that caught residents and tourists by surprise. The immense volume of water that churned down the narrow Big Thompson Canyon scoured the river channel and destroyed everything in its path, including 418 homes, 52 businesses, numerous bridges, paved and unpaved roads, power and telephone lines, and many other structures. The tragedy claimed the lives of 144 people. Scores of other people narrowly escaped with their lives. The Big Thompson flood ranks among the deadliest of Colorado's recorded floods. It is one of several destructive floods in the United States that has shown the necessity of conducting research to determine the causes and effects of floods. The U.S. Geological Survey (USGS) conducts research and operates a Nationwide streamgage network to help understand and predict the magnitude and likelihood of large streamflow events such as the Big Thompson Flood. Such research and streamgage information are part of an ongoing USGS effort to reduce flood hazards and to increase public awareness.

  12. Value engineering study report on Lower East Fork Poplar Creek Project. Alternative No. 3

    SciTech Connect

    1995-08-01

    The project under study is Alternative No. 3 as identified in the Feasibility Study dated August 1994. This alternative is identified as Excavation and Disposal of Commercial/DOE, Other, and Residential Remedial Unit Soil. The assumptions used for generating baseline costs are discussed in site associated costs. It is further described as follows: Soils with mercury concentrations greater than 200 ppM in the Commercial/DOE and Other Remedial Units and greater than 180 ppM in the Residential Remedial Unit [41,300m{sup 3} (54,000yd{sup 3} a volume equivalent to approximately 6,750 dump truck loads)] would be excavated and disposed of in an approved, lined landfill at Y-12 with leachate collection and possible pretreatment of the leachate before discharge. Because 0.6 ha (1.5 acres) of wetland would be destroyed, wetlands banking would occur, whereby a 1.8-ha (4.5-acre) wetland would be constructed on DOE-owned land near K-25. Borrow soil would be obtained from the Y-12 West End Borrow Area or from excess soil located at Y-12 landfills to fill the excavation. It is estimated that 7.3 ha (18.2 acres, and area about the size of 17 football fields) of habitat would be adversely affected. This alternative would use BMPs to minimize any adverse affects and to comply substantively with regulatory requirements.

  13. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2002-12-01

    The Little Thompson Observatory is the first community-built observatory that is part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. A committee of teachers and administrators from the Thompson School District have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." In addition, this past summer our program became an accredited course by Colorado State University. Our next project is to partner with the Discovery Center Science Museum and Colorado State University to provide additional teacher education programs. Our training materials have also been shared with TIE/Mt. Wilson, NASA Goddard and Howard University, which are working together to develop a similar teacher education program.

  14. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2003-05-01

    The Little Thompson Observatory is the first community-built observatory that is part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. A committee of teachers and administrators from the Thompson School District have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." In addition, this past summer our program became an accredited course by Colorado State University. Our next project is to partner with the Discovery Center Science Museum and Colorado State University to provide additional teacher education programs. Our training materials have also been shared with TIE/Mt. Wilson, NASA Goddard and Howard University, which are working together to develop a similar teacher education program.

  15. Wildlife and Wildlife Habitat Loss Assessment at Hills Creek Dam and Reservoir Project, Middle Fork Willamette River, Oregon, 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Hills Creek Dam and Reservoir Project on the Middle Fork Willamette River, Oregon, to determine losses or gains resulting from the development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1944, 1964, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Fifteen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Hills Creek Project extensively altered or affected 4662 acres of land and river in the Middle Fork Willamette River drainage. Impacts to wildlife centered around the loss of 2694 acres of old-growth forest and 207 acres of riparian habitat. Impacts resulting from the Hills Creek Project included the loss of winter range for Roosevelt elk, and the loss of year-round habitat for black-tailed deer, black bear, cougar, river otter, beaver, ruffed grouse, spotted owl, and other nongame species. Bald eagle and osprey were benefited by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Hills Creek Project, losses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  16. Wildlife and Wildlife Habitat Loss Assessment Summary at Lookout Point Dam and Reservoir Project, Middle Fork Willamette River, Oregon; 1985 Final Report.

    SciTech Connect

    Bedrossian, K.L.; Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Lookout Point Dam and Reservoir Project on the Middle Fork Willamette River, Oregon, to determine losses or gains resulting from development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1944, 1956, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Seventeen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Lookout Point Project extensively altered or affected 6790 acres of land and river in the Middle Fork Willamette River drainage. Impacts to wildlife centered around the loss of 724 acres of old-growth conifer forest and 118 acres of riparian habitat. Impacts resulting from the Lookout Point Project included the loss of winter range for Roosevelt elk, and the loss of year-round habitat for black-tailed deer, western gray squirrel, red fox, mink, beaver, ruffed grouse, ring-necked pheasant, California quail, spotted owl, and other nongame species. Bald eagle and osprey were benefitted by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Lookout Point Project. Loses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  17. Technology to the People: Kirsten Thompson--Milwaukee Public Library

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    Considering Kirsten Thompson's career in the Milwaukee Public Library (MPL), it is no surprise that as an undergraduate she studied both computer programming and psychology. The combination is the driving force behind her multiple projects at MPL, which have all focused on bringing technology to users. "Having the computers is great, but you need…

  18. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.; Sackett, C.

    2001-12-01

    The Little Thompson Observatory is believed to be the first observatory built as part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools in Colorado to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. We are honored that a committee of teachers and administrators from the Thompson School district have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Also in the past year, our training materials have been shared with NASA Goddard and Howard University, which are working together to develop a similar teacher education program.

  19. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2002-05-01

    The Little Thompson Observatory is believed to be the first of its kind, located next to a high school and accessible to other schools remotely over the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction was done completely by volunteer labor, and the observatory was built on the grounds of Berthoud High School in northern Colorado. During 2001, we averaged 400-500 visitors per month. We are grateful to have received a STScI IDEAS grant to provide teacher training workshops for K-12 schools in northern Colorado to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. We are honored that a committee of teachers and administrators from the Thompson School district have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Also in the past year, our training materials have been shared with NASA Goddard and Howard University, which are working together to develop a similar teacher education program. Our next goal is to add solar observing capability! Please visit our website at www.starkids.org.

  20. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2004-05-01

    The Little Thompson Observatory is the first community-built E/PO observatory that is accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. The observatory is located on the grounds of Berthoud High School in northern Colorado. The observatory will celebrate its fifth anniversary in summer 2004, and we are planning to expand the building to accommodate our growing number of visitors! We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. We have recently submitted ROSS E/PO proposals toward future teacher programs. A committee of teachers and administrators from the Thompson School District selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Our program is also accredited by Colorado State University.

  1. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2003-12-01

    The Little Thompson Observatory is the first community-built observatory that is part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. A committee of teachers and administrators from the Thompson School District selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Our program is also accredited by Colorado State University.

  2. Donald O. Thompson: A remembrance

    NASA Astrophysics Data System (ADS)

    Achenbach, J. D.; Alers, G.; Schmerr, Lester W., Jr.; Bond, Leonard J.

    2014-02-01

    This paper was developed from the remarks delivered to honor Don Thompson by the banquet speakers at the 40th QNDE meeting, July 2013. Don died peacefully at his home just days later on July 29th after a two year battle with cancer. "Don was a tenacious fighter for what he believed in, and his vision and perseverance did much to establish NDE in both the US and wider global R&D community. He will be greatly missed by his many friends and colleagues in the NDE community".

  3. Environmental compliance plan for the Lower East Fork Poplar Creek Remedial Action Project at Oak Ridge, Tennessee

    SciTech Connect

    1996-07-01

    Remedial action for Lower East Fork Poplar Creek, as defined by the Record of Decision, requires that soil contaminated with >400 ppM mercury be excavated and disposed. Based on the remediation goal, soil will be excavated from areas located at the NOAA site and the Bruner site and disposed at the Industrial Landfill V at the Y-12 Plant. Objective is to minimize the risk to human health and the environment from contaminated soil in the lower EFPC floodplain pursuant to CERCLA and the Federal Facility Agreement (DOE 1992).

  4. An archaeological reconnaissance of a 14 mile section of the East Fork Poplar Creek for the Environmental Restoration Project, Anderson and Roane Counties, Tennessee

    SciTech Connect

    DuVall, G.D.

    1993-01-01

    At the request of the US Army Corps of Engineers, Nashville District, Nashville, Tennessee, an archaeological reconnaissance of the potential impact areas of the Environmental Restoration Project (ERP) along the East Fork Poplar Creek was conducted during the period December 16, 1991, and March 3, 1992. The reconnaissance was conducted in response to environmental evaluations as a result of the accidental spillage of approximately 293,000 pounds of mercury, radionuclides, heavy metals and other compounds. The reconnaissance to assess adverse impacts to cultural resources located within the boundaries of Federally-licensed, permitted, funded or assisted projects was conducted in compliance with the National Historic Preservation Act of 1966, National Environmental Policy Act of 1969 and Executive Order 11593.

  5. Wildlife and Wildlife Habitat Loss Assessment at Cougar Dam and Reservoir Project, South Fork McKenzie River, Oregon; 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Cougar Dam and Reservoir Project on the South Fork McKenzie River, Oregon, to determine losses or gains resulting from the development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1953, 1965, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Fifteen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Cougar Project extensively altered or affected 3096 acres of land and river in the McKenzie River drainage. Impacts to wildlife centered around the loss of 1587 acres of old-growth conifer forest and 195 acres of riparian hardwoods. Impacts resulting from the Cougar Project included the loss of winter range for Roosevelt elk, and the loss of year-round habitat for black-tailed deer, black bear, cougar, river otter, beaver, spotted owl, and other nongame species. Bald eagle and osprey were benefited by an increase in foraging habitat. The potential of the effected area to support wildlife was greatly altered as a result of the Cougar Project. Loses or grains in the potential of the habitat to support wildlife will exist over the life of the project.

  6. The Little Thompson Observatory's Astronomy Education Programs

    NASA Astrophysics Data System (ADS)

    Schweitzer, Andrea E.

    2007-12-01

    The Little Thompson Observatory is a community-built E/PO observatory and is a member of the Telescopes in Education (TIE) project. The observatory is located on the grounds of Berthoud High School in northern Colorado. Annually we have approximately 5,000 visitors, which is roughly equal to the population of the small town of Berthoud, CO. This past year, we have used the funding from our NASA ROSS E/PO grant to expand our teacher workshop programs, and included the baseball-sized meteorite that landed in Berthoud three years ago. Our teacher programs have involved scientists from the Southwest Research Institute and from Fiske Planetarium at CU-Boulder. We thank the NASA ROSS E/PO program for providing this funding! We also held a Colorado Project ASTRO-GEO workshop, and the observatory continues to make high-school astronomy courses available to students from the surrounding school districts. Statewide, this year we helped support the development and construction of three new educational observatories in Colorado, located in Estes Park, Keystone, and Gunnison. The LTO is grateful to have received the recently-retired 24-inch telescope from Mount Wilson Observatory as part of the TIE program. To provide a new home for this historic telescope, we have doubled the size of the observatory and are building a second dome (all with volunteer labor). During 2008 we plan to build a custom pier and refurbish the telescope.

  7. Philip Thompson: Pages from a Scientist's Life.

    NASA Astrophysics Data System (ADS)

    Lewis, John M.

    1996-01-01

    Philip Thompson (1922-94) pioneered innovative approaches to weather analysis and prediction that blended determinism and probability. He generally posed problems in terms of simplified dynamics that were amenable to analytic solution. His preciseness in problem formulation and presentation in a forceful didactical manner are linked to his early home-schooling and experiences with a coterie of young intellectuals. Four of Thompson's contributions are examined with the intention of highlighting their impact on the current state of operational analysis and prediction.

  8. The Little Thompson Observatory's Astronomy Education Programs

    NASA Astrophysics Data System (ADS)

    Schweitzer, Andrea E.

    2008-05-01

    The Little Thompson Observatory is a community-built E/PO observatory and is a member of the Telescopes in Education (TIE) project. The observatory is located on the grounds of Berthoud High School in northern Colorado. Annually we have approximately 5,000 visitors, which is roughly equal to the population of the small town of Berthoud, CO. In spring 2008, we offered a special training session to boost participation in the GLOBE at Night international observing program. During 2005-2007 we used the funding from our NASA ROSS E/PO grant to expand our teacher workshop programs, and included the baseball-sized meteorite that landed in Berthoud four years ago. Our teacher programs are ongoing, and include scientists from the Southwest Research Institute and from Fiske Planetarium at CU-Boulder. We thank the NASA ROSS E/PO program for providing this funding! Statewide, we are a founding member of Colorado Project ASTRO-GEO, and the observatory offers high-school astronomy courses to students from the surrounding school districts. We continue to support the development and construction of three new educational observatories in Colorado, located in Estes Park, Keystone and Gunnison. The LTO is grateful to have received the retired 24-inch telescope from Mount Wilson Observatory as part of the TIE program. To provide a new home for this historic telescope, we have doubled the size of the observatory and are building a second dome (almost all construction done with volunteer labor). During 2008 we will be building a custom pier and refurbishing the telescope.

  9. Forks in the Road: The Many Paths of Arts Alumni--Strategic National Arts Alumni Project 2010 Findings

    ERIC Educational Resources Information Center

    Strategic National Arts Alumni Project, 2011

    2011-01-01

    More than 120,000 visual and performing arts degrees are granted each year (Americans for the Arts, 2010) and stereotypes abound about what happens to these people. One common view is that few make a living doing art and are dissatisfied with their education and career opportunities. Findings from the Strategic National Arts Alumni Project (SNAAP)…

  10. Taking the thrombin "fork".

    PubMed

    Mann, Kenneth G

    2010-07-01

    The proverb that probably best exemplifies my career in research is attributable to Yogi Berra (http://www.yogiberra.com/), ie, "when you come to a fork in the road ... take it." My career is a consequence of chance interactions with great mentors and talented students and the opportunities provided by a succession of ground-breaking improvements in technology. PMID:20554951

  11. Replication-Fork Dynamics

    PubMed Central

    Duderstadt, Karl E.; Reyes-Lamothe, Rodrigo; van Oijen, Antoine M.; Sherratt, David J.

    2014-01-01

    The proliferation of all organisms depends on the coordination of enzymatic events within large multiprotein replisomes that duplicate chromosomes. Whereas the structure and function of many core replisome components have been clarified, the timing and order of molecular events during replication remains obscure. To better understand the replication mechanism, new methods must be developed that allow for the observation and characterization of short-lived states and dynamic events at single replication forks. Over the last decade, great progress has been made toward this goal with the development of novel DNA nanomanipulation and fluorescence imaging techniques allowing for the direct observation of replication-fork dynamics both reconstituted in vitro and in live cells. This article reviews these new single-molecule approaches and the revised understanding of replisome operation that has emerged. PMID:23881939

  12. Teacher Hiring, Support and Evaluation in Thompson School District, CO. Executive Summary

    ERIC Educational Resources Information Center

    New Teacher Project, 2008

    2008-01-01

    In the spring of 2008, The New Teacher Project (TNTP) partnered with the Thompson School District (TSD) in Loveland, Colorado, to analyze current teacher staffing practices in the district and make recommendations to further build the concentration of highly effective teachers in TSD schools. TNTP's analysis of policy and practice in TSD…

  13. 3. View of Clark Fork Vehicle Bridge facing southwest. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. View of Clark Fork Vehicle Bridge facing southwest. Bridge from north shore of Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  14. 4. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shoreof Clark Fork River showing 4 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  15. 2. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  16. 1. View of Clark Fork Vehicle Bridge facing west. Panorama ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Clark Fork Vehicle Bridge facing west. Panorama showing the entire span of bridge from north shore of the Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  17. 7. View of Clark Fork Vehicle Bridge facing northwest. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. View of Clark Fork Vehicle Bridge facing northwest. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  18. Paresev in flight with pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1964-01-01

    This movie clip runs 37 seconds in length and begins with a shot from the chase plane of NASA Dryden test pilot Milt Thompson at the controls of the Paresev, then the onboard view from the pilot's seat and finally bringing the Paresev in for a landing on the dry lakebed at Edwards AFB. The Paresev (Paraglider Rescue Vehicle) was an indirect outgrowth of kite-parachute studies by NACA Langley engineer Francis M. Rogallo. In early 1960's the 'Rogallo wing' seemed an excellent means of returning a spacecraft to Earth. The delta wing design was patented by Mr. Rogallo. In May 1961, Robert R. Gilruth, director of the NASA Space Task Group, requested studies of an inflatable Rogallo-type 'Parawing' for spacecraft. Several companies responded; North American Aviation, Downey, California, produced the most acceptable concept and development was contracted to that company. In November 1961 NASA Headquarters launched a paraglider development program, with Langley doing wind tunnel studies and the NASA Flight Research Center supporting the North American test program. The North American concept was a capsule-type vehicle with a stowed 'parawing' that could be deployed and controlled from within for a landing more like an airplane instead of a 'splash down' in the ocean. The logistics became enormous and the price exorbitant, plus NASA pilots and engineers felt some baseline experience like building a vehicle and flying a Parawing should be accomplished first. The Paresev (Paraglider Research Vehicle) was used to gain in-flight experience with four different membranes (wings), and was not used to develop the more complicated inflatable deployment system. The Paresev was designed by Charles Richard, of the Flight Research Center Vehicle and System Dynamics Branch, with the rest of the team being: engineers, Richard Klein, Gary Layton, John Orahood, and Joe Wilson; from the Maintenance and Manufacturing Branch: Frank Fedor, LeRoy Barto; Victor Horton as Project Manager, with

  19. The Interpretive Approach to Religious Education: Challenging Thompson's Interpretation

    ERIC Educational Resources Information Center

    Jackson, Robert

    2012-01-01

    In a recent book chapter, Matthew Thompson makes some criticisms of my work, including the interpretive approach to religious education and the research and activity of Warwick Religions and Education Research Unit. Against the background of a discussion of religious education in the public sphere, my response challenges Thompson's account,…

  20. Paresev in flight with pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1964-01-01

    This movie clip runs 37 seconds in length and begins with a shot from the chase plane of NASA Dryden test pilot Milt Thompson at the controls of the Paresev, then the onboard view from the pilot's seat and finally bringing the Paresev in for a landing on the dry lakebed at Edwards AFB. The Paresev (Paraglider Rescue Vehicle) was an indirect outgrowth of kite-parachute studies by NACA Langley engineer Francis M. Rogallo. In early 1960's the 'Rogallo wing' seemed an excellent means of returning a spacecraft to Earth. The delta wing design was patented by Mr. Rogallo. In May 1961, Robert R. Gilruth, director of the NASA Space Task Group, requested studies of an inflatable Rogallo-type 'Parawing' for spacecraft. Several companies responded; North American Aviation, Downey, California, produced the most acceptable concept and development was contracted to that company. In November 1961 NASA Headquarters launched a paraglider development program, with Langley doing wind tunnel studies and the NASA Flight Research Center supporting the North American test program. The North American concept was a capsule-type vehicle with a stowed 'parawing' that could be deployed and controlled from within for a landing more like an airplane instead of a 'splash down' in the ocean. The logistics became enormous and the price exorbitant, plus NASA pilots and engineers felt some baseline experience like building a vehicle and flying a Parawing should be accomplished first. The Paresev (Paraglider Research Vehicle) was used to gain in-flight experience with four different membranes (wings), and was not used to develop the more complicated inflatable deployment system. The Paresev was designed by Charles Richard, of the Flight Research Center Vehicle and System Dynamics Branch, with the rest of the team being: engineers, Richard Klein, Gary Layton, John Orahood, and Joe Wilson; from the Maintenance and Manufacturing Branch: Frank Fedor, LeRoy Barto; Victor Horton as Project Manager, with

  1. Tuning Forks and Monitor Screens.

    ERIC Educational Resources Information Center

    Harrison, M. A. T.

    2000-01-01

    Defines the vibrations of a tuning fork against a computer monitor screen as a pattern that can illustrate or explain physical concepts like wave vibrations, wave forms, and phase differences. Presents background information and demonstrates the experiment. (Author/YDS)

  2. 76 FR 50171 - Notice of Intent To Prepare an Environmental Impact Statement for the Henrys Fork Salinity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-12

    ... the Henrys Fork Salinity Control Project Plan, Sweetwater and Uinta Counties, WY; Daggett and Summit... Statement (EIS) for the Henrys Fork Salinity Control Project Plan (SCPP). The NRCS will be the lead agency... Improvements'' alternative assumes a salinity control project will be implemented. Existing financial...

  3. 22. View of Clark Fork Vehicle Bridge facing downwest side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View of Clark Fork Vehicle Bridge facing down-west side. Looking at road deck and vertical laced channel. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  4. 11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost span. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  5. 19. View of Clark Fork Vehicle Bridge facing north. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. View of Clark Fork Vehicle Bridge facing north. Looking at north abutment and underside of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  6. 20. View of Clark Fork Vehicle Bridge facing up. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. View of Clark Fork Vehicle Bridge facing up. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  7. 8. View of Clark Fork Vehicle Bridge facing southwest. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. View of Clark Fork Vehicle Bridge facing southwest. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  8. 12. View of Clark Fork Vehicle Bridge facing south. Approach ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. View of Clark Fork Vehicle Bridge facing south. Approach from the north road. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  9. 21. View of Clark Fork Vehicle Bridge facing west. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View of Clark Fork Vehicle Bridge facing west. Looking at bridge deck, guard rail, juncture of two bridge spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  10. 18. View of Clark Fork Vehicle Bridge facing north. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of Clark Fork Vehicle Bridge facing north. Looking at north concrete abutment and timber stringers. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  11. Complete genome sequence of salmonella enterica subsp. enterica Serovar Thompson Strain RM6836

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Salmonella enterica subsp. enterica serovar Thompson (S. Thompson) strain RM6836 was isolated from lettuce in 2002. We report the complete sequence and annotation of the genome of S. Thompson strain RM6836. This is the first reported complete genome sequence for S. Thompson and will provide a point ...

  12. X-15 mock-up with test pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1993-01-01

    NASA research pilot Milt Thompson is seen here with the mock-up of X-15 #3 that was later installed at the NASA Dryden Flight Research Center, Edwards, California. Milton 0. Thompson was a research pilot, Chief Engineer and Director of Research Projects during a long career at the NASA Dryden Flight Research Center. Thompson was hired as an engineer at the flight research facility on 19 March 1956, when it was still under the auspices of NACA. He became a research pilot on 25 May 1958. Thompson was one of the 12 NASA, Air Force, and Navy pilots to fly the X-15 rocket-powered research aircraft between 1959 and 1968. He began flying X-15s on 29 October 1963. He flew the aircraft 14 times during the following two years, reaching a maximum speed of 3723 mph (Mach 5.42) and a peak altitude of 214,100 feet on separate flights. (On a different flight, he reached a Mach number of 5.48 but his mph was only 3712.) Thompson concluded his active flying career in 1968, becoming Director of Research Projects. In 1975 he was appointed Chief Engineer and retained the position until his death on 8 August 1993. The X-15 was a rocket powered aircraft 50 ft long with a wingspan of 22 ft. It was a missile-shaped vehicle with an unusual wedge-shaped vertical tail, thin stubby wings, and unique side fairings that extended along the side of the fuselage. The X-15 weighed about 14,000 lb empty and approximately 34,000 lb at launch. The XLR-99 rocket engine, manufactured by Thiokol Chemical Corp., was pilot controlled and was capable of developing 57,000 lb of thrust. North American Aviation built three X-15 aircraft for the program. The X-15 research aircraft was developed to provide in-flight information and data on aerodynamics, structures, flight controls, and the physiological aspects of high-speed, high-altitude flight. A follow on program used the aircraft as a testbed to carry various scientific experiments beyond the Earth's atmosphere on a repeated basis. For flight in the dense

  13. X-15 mock-up with test pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1993-01-01

    NASA research pilot Milt Thompson stands next to a mock-up of X-15 number 3 that was later installed at the NASA Dryden Flight Research Center, Edwards, California. Milton 0. Thompson was a research pilot, Chief Engineer and Director of Research Projects during a long career at the NASA Dryden Flight Research Center. Thompson was hired as an engineer at the flight research facility on 19 March 1956, when it was still under the auspices of NACA. He became a research pilot on 25 May 1958. Thompson was one of the 12 NASA, Air Force, and Navy pilots to fly the X-15 rocket-powered research aircraft between 1959 and 1968. He began flying X-15s on 29 October 1963. He flew the aircraft 14 times during the following two years, reaching a maximum speed of 3723 mph (Mach 5.42) and a peak altitude of 214,100 feet on separate flights. Thompson concluded his active flying career in 1968, becoming Director of Research Projects. In 1975 he was appointed Chief Engineer and retained the position until his death on 8 August 1993. The X-15 was a rocket-powered aircraft 50 ft long with a wingspan of 22 ft. It was a missile-shaped vehicle with an unusual wedge-shaped vertical tail, thin stubby wings, and unique side fairings that extended along the side of the fuselage. The X-15 weighed about 14,000 lb empty and approximately 34,000 lb at launch. The XLR-99 rocket engine, manufactured by Thiokol Chemical Corp., was pilot controlled and was capable of developing 57,000 lb of thrust. North American Aviation built three X-15 aircraft for the program. The X-15 research aircraft was developed to provide in-flight information and data on aerodynamics, structures, flight controls, and the physiological aspects of high-speed, high-altitude flight. A follow-on program used the aircraft as a testbed to carry various scientific experiments beyond the Earth's atmosphere on a repeated basis. For flight in the dense air of the usable atmosphere, the X-15 used conventional aerodynamic controls such as

  14. 1. Historic American Buildings Survey Thompson & West Illustrator in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey Thompson & West Illustrator in History of Sacramento County Photo Copy 1960 NORTH ELEVATION (1880) - Albert Gallatin House, 1527 H Street, Sacramento, Sacramento County, CA

  15. 1. Historic American Building Survey Thompson & West Illustrated History ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Building Survey Thompson & West Illustrated History of Sacramento Co. Drawing of 1880 Plan & Rephoto 1960 NORTHEAST CORNER AND GENERAL PLAN - Crocker Art Gallery, 216 O Street, Sacramento, Sacramento County, CA

  16. 77 FR 34337 - Uinta-Wasatch-Cache National Forest; Evanston-Mountain View Ranger District; Utah; Smiths Fork...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ... Forest Service Uinta-Wasatch-Cache National Forest; Evanston-Mountain View Ranger District; Utah; Smiths... submitting a correction to the notice of intent for the Smiths Fork Vegetation Restoration Project published... period for the Smiths Fork Vegetation Restoration Project has been extended. Comments concerning...

  17. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning fork is a mechanical device...

  18. HOWARD FORK ACID ROCK DRAINAGE SOURCE INTERCEPTION STUDY; HOWARD FORK OF THE SAN MIGUEL RIVER NEAR OPHIR, COLORADO

    EPA Science Inventory

    This project proposes to analyze regional hydrogeology as it relates to mine workings which discharge significant heavy metals into the Howard Fork of the San Miguel River and recommend strategies to intercept and divert water away from mineralized zones. The study also includes...

  19. ADAPTATION OPPORTUNITIES FOR SURFACE WATER RESOURCES IN THE ROARING FORK WATERSHED, COLORADO.

    EPA Science Inventory

    This project will assess climate-related impacts to the Roring Fork River (near Aspen, Colorado) and identify adaptive opportunities for surface water users, to support a larger global change assessment by the city of Aspen, CO (the Canary Initiative).

  20. Estimated Loads of Suspended Sediment and Selected Trace Elements Transported through the Milltown Reservoir Project Area Before and After the Breaching of Milltown Dam in the Upper Clark Fork Basin, Montana, Water Year 2008

    USGS Publications Warehouse

    Lambing, John H.; Sando, Steven K.

    2009-01-01

    This report presents estimated daily and cumulative loads of suspended sediment and selected trace elements transported during water year 2008 at three streamflow-gaging stations that bracket the Milltown Reservoir project area in the upper Clark Fork basin of western Montana. Milltown Reservoir is a National Priorities List Superfund site where sediments enriched in trace elements from historical mining and ore processing have been deposited since the construction of Milltown Dam in 1907. Milltown Dam was breached on March 28, 2008, as part of Superfund remedial activities to remove the dam and contaminated sediment that had accumulated in Milltown Reservoir. The estimated loads transported through the project area during the periods before and after the breaching of Milltown Dam, and for the entire water year 2008, were used to quantify the net gain or loss (mass balance) of suspended sediment and trace elements within the project area during the transition from a reservoir environment to a free-flowing river. This study was done in cooperation with the U.S. Environmental Protection Agency. Streamflow during water year 2008 compared to long-term streamflow, as represented by the record for Clark Fork above Missoula (water years 1930-2008), generally was below normal (long-term median) from about October 2007 through April 2008. Sustained runoff started in mid-April, which increased flows to near normal by mid-May. After mid-May, flows sharply increased to above normal, reaching a maximum daily mean streamflow of 16,800 cubic feet per second (ft3/s) on May 21, which essentially equaled the long-term 10th-exceedance percentile for that date. Flows substantially above normal were sustained through June, then decreased through the summer and reached near-normal by August. Annual mean streamflow during water year 2008 (3,040 ft3/s) was 105 percent of the long-term mean annual streamflow (2,900 ft3/s). The annual peak flow (17,500 ft3/s) occurred on May 21 and was 112

  1. 5. View of Clark Fork Vehicle Bridge facing east. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View of Clark Fork Vehicle Bridge facing east. Bridge from south shore of Clark Fork River-southernmost span. 1900-era Northern Pacific Railway Bridge in background. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  2. Crafts, Boys, Ernest Thompson Seton, and the Woodcraft Movement

    ERIC Educational Resources Information Center

    Chalmers, F. Graeme; Dancer, Andrea A.

    2008-01-01

    This article examines early influences on art education for boys (Chalmers & Dancer, 2007) in areas traditionally labeled as crafts. Under review is the work of Ernest Thompson Seton, artist, naturalist, storyteller, author, philosopher, crusader for and supporter of indigenous American Indian ways of knowing, and a co-founder of the Boy Scouts of…

  3. On Happiness and High Achievement: An Interview with Michael Thompson

    ERIC Educational Resources Information Center

    National Association of Independent Schools (NAIS) (NJ), 2006

    2006-01-01

    Michael G. Thompson knows independent schools. He attended them in elementary and secondary school, and has worked in them as a psychologist. He consults with more than 30 schools a year, addressing a myriad of issues related to complex human interaction. He has written often in "Independent School" about everything from understanding the social…

  4. 2. Historic American Building Survey History of Sacramento County Thompson ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Building Survey History of Sacramento County Thompson & West Illustration Original 1880 Rephoto 1960 N.W. Corner of 3rd + P ST., SOUTH HOUSE (Property of Mrs. E.B. Crocker, 3rd & P St.) - Crocker Art Gallery, 216 O Street, Sacramento, Sacramento County, CA

  5. On nonsingular potentials of Cox-Thompson inversion scheme

    SciTech Connect

    Palmai, Tamas; Apagyi, Barnabas

    2010-02-15

    We establish a condition for obtaining nonsingular potentials using the Cox-Thompson inverse scattering method with one phase shift. The anomalous singularities of the potentials are avoided by maintaining unique solutions of the underlying Regge-Newton integral equation for the transformation kernel. As a by-product, new inequality sequences of zeros of Bessel functions are discovered.

  6. On nonsingular potentials of Cox-Thompson inversion scheme

    NASA Astrophysics Data System (ADS)

    Pálmai, Tamás; Apagyi, Barnabás

    2010-02-01

    We establish a condition for obtaining nonsingular potentials using the Cox-Thompson inverse scattering method with one phase shift. The anomalous singularities of the potentials are avoided by maintaining unique solutions of the underlying Regge-Newton integral equation for the transformation kernel. As a by-product, new inequality sequences of zeros of Bessel functions are discovered.

  7. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  8. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  9. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  10. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  11. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  12. Bruce Thompson: Adventures and advances in ultrasonic backscatter

    NASA Astrophysics Data System (ADS)

    Margetan, Frank J.

    2012-05-01

    Over the course of his professional career Dr. R. Bruce Thompson published several hundred articles on non-destructive evaluation, the majority dealing with topics in ultrasonics. One longtime research interest of Dr. Thompson, with applications both to microstructure characterization and defect detection, was backscattered grain noise in metals. Over a 20 year period he led a revolving team of staff members and graduate students investigating various aspects of ultrasonic backscatter. As a member of that team I had the privilege of working along side Dr. Thompson for many years, serving as a sort of Dr. Watson to Bruce's Sherlock Holmes. This article discusses Dr. Thompson's general approaches to modeling backscatter, the research topics he chose to explore to systematically elucidate a better understanding of the phenomena, and the many contributions to the field achieved under his leadership. The backscatter work began in earnest around 1990, motivated by a need to improve inspections of aircraft engine components. At that time Dr. Thompson launched two research efforts. The first led to the heuristic Independent Scatterer Model which could be used to estimate the average grain noise level that would be seen in any given ultrasonic inspection. There the contribution from the microstructure was contained in a measureable parameter known as the Figure-of-Merit or FOM. The second research effort, spearheaded by Dr. Jim Rose, led to a formal relationship between FOM and details of the metal microstructure. The combination of the Independent Scattering Model and Rose's formalism provided a powerful tool for investigating backscatter in metals. In this article model developments are briefly reviewed and several illustrative applications are discussed. These include: the determination of grain size and shape from ultrasonic backscatter; grain noise variability in engine-titanium billets and forgings; and the design of ultrasonic inspection systems to improve defect

  13. South Fork Clearwater River Habitat Enhancement, Nez Perce National Forest.

    SciTech Connect

    Siddall, Phoebe

    1992-04-01

    In 1984, the Nez Perce National forest and the Bonneville Power Administration entered into a contractual agreement which provided for improvement of spring chinook salmon and summer steelhead trout habitat in south Fork Clearwater River tributaries. Project work was completed in seven main locations: Crooked River, Red River, Meadow Creek Haysfork Gloryhole, Cal-Idaho Gloryhole, Fisher Placer and Leggett Placer. This report describes restoration activities at each of these sites.

  14. M2-F1 on lakebed with pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1963-01-01

    adequate for the roughly 400 car tows that got the M2-F1 airborne to prove it could fly safely and to train pilots before they were towed behind a C-47 aircraft and released. These initial car-tow tests produced enough flight data about the M2-F1 to proceed with flights behind the C-47 tow plane at greater altitudes. The C-47 took the craft to an altitude of 12,000 where free flights back to Rogers Dry Lake began. Pilot for the first series of flights of the M2-F1 was NASA research pilot Milt Thompson. Typical glide flights with the M2-F1 lasted about two minutes and reached speeds of 110 to l20 mph. A small solid landing rocket, referred to as the 'instant L/D rocket,' was installed in the rear base of the M2-F1. This rocket, which could be ignited by the pilot, provided about 250 pounds of thrust for about 10 seconds. The rocket could be used to extend the flight time near landing if needed. More than 400 ground tows and 77 aircraft tow flights were carried out with the M2-F1. The success of Dryden's M2-F1 program led to NASA's development and construction of two heavyweight lifting bodies based on studies at NASA's Ames and Langley research centers--the M2-F2 and the HL-10, both built by the Northrop Corporation, and the U.S. Air Force's X-24 program, with an X-24A and -B built by Martin. The Lifting Body program also heavily influenced the Space Shuttle program. The M2-F1 program demonstrated the feasibility of the lifting body concept for horizontal landings of atmospheric entry vehicles. It also demonstrated a procurement and management concept for prototype flight test vehicles that produced rapid results at very low cost (approximately $50,000, excluding salaries of government employees assigned to the project).

  15. Research reactor fork users manual

    SciTech Connect

    Hsue, S.T.; Menlove, H.O.; Bosler, G.E.; Dye, H.R.; Walton, G.; Halbig, J.K.; Siebelist, R.

    1993-11-01

    This manual describes the design features and operating characteristics of the research reactor fork. The system includes an ion chamber for gross gamma-ray counting, fission chambers for neutron counting, and a collimated high-resolution spectroscopy system for gamma-ray measurements. The neutron and ion chamber measurements are designed to be made underwater in spent-fuel cooling ponds. The neutron and gamma-ray detectors have been designed with high efficiencies to accommodate the relatively low emission rates of neutrons and gamma rays from low-burnup, research-type reactor fuel. This manual presents the design, performance, and test results for the system.

  16. Training Guidelines: Fork Lift Truck Driving.

    ERIC Educational Resources Information Center

    Ceramics, Glass, and Mineral Products Industry Training Board, Harrow (England).

    This manual of operative training guidelines for fork lift truck driving has been developed by the Ceramics, Glass and Mineral Products Industry Training Board (Great Britain) in consultation with a number of firms which manufacture fork lift trucks or which already have training--programs for their use. The purpose of the guidelines is to assist…

  17. Fifth-wheel fork truck adapter

    NASA Technical Reports Server (NTRS)

    Smith, P. L.

    1969-01-01

    Standard fifth wheel mounted on a rectangular steel structure adapted for use with a fork lift truck provides a fast, safe, and economical way of maneuvering semitrailers in close quarters at plants and warehouses. One operator can move and locate a semitrailer without dismounting from a fork lift truck.

  18. 75 FR 25197 - Shasta Trinity National Forest, South Fork Management Unit, California Salt Timber Harvest and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ... Forest Service Shasta Trinity National Forest, South Fork Management Unit, California Salt Timber Harvest... statement for the Salt Timber Harvest and Fuels Reduction Project (Salt Project). A supplemental environmental impact statement will be prepared for the Salt Project to supplement wildlife management...

  19. Acoustic Doppler current profiling from the JGOFS Arabian Sea cruises aboard the RV T.G. Thompson

    SciTech Connect

    Kim, H.S.; Flagg, C.N.; Shi, Y.

    1996-12-01

    Acoustic Doppler current profiler (ADCP) data is part of the core data for the US JGOFS Arabian Sea project, along with hydrographic and nutrient data. Seventeen cruises are scheduled to take place between September 1994 and January 1996 on the R/V T.G. Thompson. Seven of the cruises follow a standard cruise track, taking hydrographic, chemical and biological measurements. The rest of the cruises, which take place generally within the standard cruise region defined by a set track, are for the deployment and recovery of moored equipment and towing of a SeaSoar. Detailed description of ADCP hardware, the AutoADCP data acquisition system, and the collection of navigation and compass data on the Thompson is documented in Section 2. Followed by data collection for each cruise together with a cruise track, Section 3 presents the processing and analysis of velocity and acoustic backscatter intensity data. Section 5 shows results of profile quality diagnosis.

  20. Sarcophilia, cremation and Sir Henry Thompson (1820-1904).

    PubMed

    Jellinek, E H

    2009-11-01

    Sarcophilia, a neologism for an attachment to human remains, is set in a review of the history of the disposal of the dead. The ancient practice of cremation was relaunched late in the 19th century by the urological surgeon cum social reformer Sir Henry Thompson. He was stimulated by Edwin Chadwick and Charles Dickens, and by Charles Darwin's observations on the earthworm. Sarcophilia is the reason for the controversial Human Tissue Act of 2004. PMID:20029076

  1. 1976 Big Thompson Flood, Colorado - Thirty Years Later

    USGS Publications Warehouse

    Jarrett, Robert D.; Costa, John E.

    2006-01-01

    In the early evening of Saturday, July 31, 1976, a large stationary thunderstorm released as much as 7.5 inches of rainfall in about an hour (about 12 inches in a few hours) in the middle reaches of the Big Thompson River Basin and to a lesser extent in parts of the Cache la Poudre River Basin. In steep mountain terrain with thin or no soil, this large amount of rainfall in such a short period of time produced a flash flood that caught residents and tourists by surprise. The sudden flood that churned down the narrow Big Thompson Canyon scoured the river channel that night, caused over $35 million in damages (1977 dollars) to 418 homes and businesses, many mobile homes, 438 automobiles, numerous bridges, paved and unpaved roads, power and telephone lines, and many other structures. The tragedy claimed the lives of 144 people, including two law enforcement officers trying to evacuate people in danger, and there were 250 reported injuries. Scores of other people narrowly escaped with their lives. More than 800 people were evacuated by helicopter the following morning. This fact sheet presents a summary of the hydrologic conditions of the 1976 flood, describes some of the advances in U.S. Geological Survey (USGS) flood science as a consequence of this disaster, and provides a reminder that extreme floods like the 1976 Big Thompson flood have occurred in other locations in Colorado in the past and will occur again.

  2. South Fork Holston River basin 1988 biomonitoring

    SciTech Connect

    Saylor, C.F.; Ahlstedt, S.A.

    1990-06-01

    There is concern over the effects of shifts in land use use practices on the aquatic fauna of streams in the South Fork Holston River basin in northwestern North Carolina and southwestern Virginia. Trout reproduction has noticeably declined in the Watauga River subbasin. The Watauga River and Elk River subbasins have been subjected to commercial and resort development. The Middle fork Holston River and the upper South Fork Holston River subbasins have been affected by agricultural and mining activities, respectively (Cox, 1986). To aid reclamation and management of the South Fork Holston basin, Tennessee Valley Authority (TVA) biologists conducted biomonitoring--including index of biotic integrity and macroinvertebrate sampling--on the Middle Fork Holston, South Fork Holston, Watauga, and Elk Rivers to assess cumulative impairment related to changes in habitat and pollutant loading in these subbasins. Biomonitoring can detect environmental degradation, help document problem areas, and assist in development of strategies for managing water quality. This report discusses the methods and materials and results of the biomonitoring of South Fork Holston River Basin. 13 refs., 5 figs., 12 tabs.

  3. 13. View of Clark Fork Vehicle Bridge facing south. Concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. View of Clark Fork Vehicle Bridge facing south. Concrete barrier blocks access. Plaque was originally located where strioed traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  4. 14. View of Clark Fork Vehicle Bridge facing north. Approach ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. View of Clark Fork Vehicle Bridge facing north. Approach from the south. Concrete barrier blocks access. Plaque was originally located where striped traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  5. 24. View of one of the plaques from Clark Fork ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. View of one of the plaques from Clark Fork Vehicle Bridge. Presently located at the Bonner County Historical Museum in Sandpoint, Idaho. A plaque was attached at each end of the bridge. Only one remains. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  6. 23. View of Clark Fork Vehicle Bridge facing upwest side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. View of Clark Fork Vehicle Bridge facing up-west side. Looking at structural connection of top chord, vertical laced channel and diagonal bars. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  7. Soil Data from a Moderately Well and Somewhat Poorly Drained Fire Chronosequence near Thompson, Manitoba, Canada

    USGS Publications Warehouse

    Manies, K.L.; Harden, J.W.; Veldhuis, Hugo; Trumbore, Sue

    2006-01-01

    The U.S. Geological Survey project Fate of Carbon in Alaskan Landscapes (FOCAL) is studying the effect of fire and soil drainage on soil carbon storage in the boreal forest. As such this group was invited to be a part of a NSF-funded project (Fire, Ecosystem and Succession - Experiment Boreal or FIRES-ExB) to study the carbon balance of sites that varied in age (time since fire) and soil drainage in the Thompson, Manitoba, Canada region. This report describes the location of our FIRES-ExB sampling sites as well as the procedures used to describe, sample, and analyze the soils. This report also contains data tables with sample related information including, but not limited to, field descriptions, bulk density, particle size distribution, moisture content, carbon (C) concentration, nitrogen (N) concentration, isotopic data for C, and major, minor and trace elemental concentration.

  8. WRNIP1 protects stalled forks from degradation and promotes fork restart after replication stress.

    PubMed

    Leuzzi, Giuseppe; Marabitti, Veronica; Pichierri, Pietro; Franchitto, Annapaola

    2016-07-01

    Accurate handling of stalled replication forks is crucial for the maintenance of genome stability. RAD51 defends stalled replication forks from nucleolytic attack, which otherwise can threaten genome stability. However, the identity of other factors that can collaborate with RAD51 in this task is poorly elucidated. Here, we establish that human Werner helicase interacting protein 1 (WRNIP1) is localized to stalled replication forks and cooperates with RAD51 to safeguard fork integrity. We show that WRNIP1 is directly involved in preventing uncontrolled MRE11-mediated degradation of stalled replication forks by promoting RAD51 stabilization on ssDNA We further demonstrate that replication fork protection does not require the ATPase activity of WRNIP1 that is however essential to achieve the recovery of perturbed replication forks. Loss of WRNIP1 or its catalytic activity causes extensive DNA damage and chromosomal aberrations. Intriguingly, downregulation of the anti-recombinase FBH1 can compensate for loss of WRNIP1 activity, since it attenuates replication fork degradation and chromosomal aberrations in WRNIP1-deficient cells. Therefore, these findings unveil a unique role for WRNIP1 as a replication fork-protective factor in maintaining genome stability. PMID:27242363

  9. Acoustic doppler current profiling from the JGOFS Arabian Sea cruises aboard the RV T.G. Thompson

    SciTech Connect

    Flagg, C.N.; Shi, Y.

    1995-04-01

    Acoustic Doppler Current Profiler (ADCP) data from the R/V T.G. THOMPSON is part of the core data for the US JGOFS Arabian Sea project along with hydrographic and nutrient data. Seventeen cruises on the THOMPSON are scheduled to take place between September 1994 and January 1996. The first of these cruises, a transit of the R/V THOMPSON into the northern Arabian Sea area from Singapore, was a calibration and training cruise that took place between September 18 and October 7, 1994. (The cruises on the THOMPSON are numbered consecutively from the ship`s commissioning with the first JOGFS cruise designated TN039.) The remaining cruises have been and will be staged from Muscat, Oman. Seven of these cruises, referred to as process cruises, will follow a set cruise track, making hydrographic, chemical and biological measurements. The remainder of the cruises while not restricted to the set cruise track, will generally stay within the region defined by the track during the deployment and retrieval of moored equipment and the towing of a SeaSoar. Each cruise will last between two weeks and one month. ADCP data will be collected on all the JGOFS Arabian Sea cruises using an autonomous data acquisition system developed for ship-of-opportunity cruises. This system, referred to as the AutoADCP, makes it possible to collect the ADCP data without the constant monitoring usually necessary and assures constant data coverage and uniform data quality. The AutoADCP system is an extension of RD Instrument`s DAS version 2.48 using enhancements made possible with ``user exit`` programs. This data report presents ADCP results from the first four JGOFS cruises, TN039 through TN042, concentrating on the data collection and processing methods.

  10. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS, AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    EPA Science Inventory

    This technical report provides a description of the field project design, quality control, the sampling protocols and analysis methodology used, and standard operating procedures for the South Fork Broad River Watershed (SFBR) Total Maximum Daily Load (TMDL) project. This watersh...

  11. Yawning, fatigue, and cortisol: expanding the Thompson Cortisol Hypothesis.

    PubMed

    Thompson, Simon B N

    2014-10-01

    Yawning and its involvement in neurological disorders has become the new scientific conundrum. Cortisol levels are known to rise during stress and fatigue; yawning may occur when we are under stress or tired. However, the link between yawning, fatigue, and cortisol has not been fully understood. Expansion of the Thompson Cortisol Hypothesis proposes that the stress hormone, cortisol, is responsible for yawning and fatigue especially in people with incomplete innervation such as multiple sclerosis. This informs our understanding of the functional importance of the brain stem region of the brain in regulating stress and fatigue. PMID:25169036

  12. South Fork Clearwater River Habitat Enhancement, Crooked and Red Rivers : Annual Report, 1989.

    SciTech Connect

    Baer, William H.

    1990-01-01

    In 1983, the Nez Perce National Forest and the Bonneville Power Administration entered into an interagency agreement to enhance and improve habitat for two anadromous fish species, spring chinook salmon (Oncorhynchus tshawyscha) and summer steelhead trout (Onchorhyncus mykiss), in the South Fork Clearwater River tributaries. The South Fork Clearwater River was dammed in 1927 for hydroelectric development. Anadromous fish runs were virtually eliminated until the dam was removed in 1962. To complicate the problem, upstream spawning and rearing habitats were severely impacted by dredge and hydraulic mining, road building, timber harvest, and over-grazing. Fish habitat improvement projects under the above contract are being carried out in two major tributaries to the South Fork Clearwater River. Both the Red River and the Crooked River projects began in 1983 and will be completed in 1990. 12 figures., 1 tab.

  13. Storm and flood of July 31-August 1, 1976, in the Big Thompson River and Cache la Poudre River basins, Larimer and Weld Counties, Colorado

    USGS Publications Warehouse

    McCain, Jerald F.; Shroba, R.R.

    1979-01-01

    PART A: Devastating flash floods swept through the canyon section of Larimer County in north-central Colorado during the night of July 31-August I, 1976, causing 139 deaths, 5 missing persons, and more than $35 million in total damages. The brunt of the storms occurred over the Big Thompson River basin between Drake and Estes Park with rainfall amounts as much as 12 inches being reported during the storm period. In the Cache la Poudre River basin to the north, a rainfall amount of 10 inches was reported for one locality while 6 inches fell over a widespread area near the central part of the basin. The storms developed when strong low-level easterly winds to the rear of a polar front pushed a moist, conditionally unstable airmass upslope into the Front Range of the Rocky Mountains. Orographic uplift released the convective instability, and light south-southeasterly winds at middle and upper levels allowed the storm complex to remain nearly stationary over the foothills for several hours. Minimal entrainment of relatively moist air at middle and upper levels, very low cloud bases, and a slightly tilted updraft structure contributed to a high precipitation efficiency. Intense rainfall began soon after 1900 MDT (Mountain Daylight Time) in the Big Thompson River and the North Fork Cache la Poudre River basins. A cumulative rainfall curve developed for Glen Comfort from radar data indicates that 7.5 inches of rain fell during the period 1930-2040 MDT on July 31. In the central part of the storm area west of Fort Collins, the heaviest rainfall began about 2200 MDT on July 31 and continued until 0100 MDT on August 1. Peak discharges were extremely large on many streams in the storm area-exceeding previously recorded maximum discharges at several locations. The peak discharge of the Big Thompson River at the gaging station at the canyon mouth, near Drake was 31,200 cubic feet per second or more than four times the previous maximum discharge of 7,600 cubic feet per second at

  14. Stability of blocked replication forks in vivo

    PubMed Central

    Mettrick, Karla A.; Grainge, Ian

    2016-01-01

    Replication of chromosomal DNA must be carried out to completion in order for a cell to proliferate. However, replication forks can stall during this process for a variety of reasons, including nucleoprotein ‘roadblocks’ and DNA lesions. In these circumstances the replisome copying the DNA may disengage from the chromosome to allow various repair processes to restore DNA integrity and enable replication to continue. Here, we report the in vivo stability of the replication fork when it encounters a nucleoprotein blockage in Escherichia coli. Using a site-specific and reversible protein block system in conjunction with the temperature sensitive DnaC helicase loader and DnaB replicative helicase, we monitored the disappearance of the Y-shaped DNA replication fork structures using neutral-neutral 2D agarose gels. We show the replication fork collapses within 5 min of encountering the roadblock. Therefore, the stalled replication fork does not pause at a block in a stable confirmation for an extended period of time as previously postulated. PMID:26490956

  15. Biochemical characteristics and thermal inhibition kinetics of polyphenol oxidase extracted from Thompson seedless grape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Polyphenol oxidase (PPO) was isolated from Thompson seedless grape (Vitis vinifera 'Thompson Seedless') and its biochemical characteristics were studied. Optimum pH and temperature for grape PPO activity were pH 6.0 and 25 degrees C with 10 mM catechol as substrate. The enzyme was heat-stable betwee...

  16. Revisiting Charles H. Thompson's Proposals for Educating Gifted African American Students, 1933-1961

    ERIC Educational Resources Information Center

    Ray, Louis

    2012-01-01

    Charles H. Thompson is best known as the founder and the first editor-in-chief of "The Journal of Negro Education" (1932-1963). Throughout his career, Thompson sought to extend educational opportunity in ways that were "for the good of Negro education as a whole." His main concern was in educating future leaders for service in African American…

  17. South Fork Latrine showing north and west sides, general view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine showing north and west sides, general view to southeast - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  18. South Fork Latrine, oblique view showing south and east sides; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, oblique view showing south and east sides; view northwest - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  19. South Fork Latrine, east elevation showing structure in context, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, east elevation showing structure in context, view west - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  20. 6. Roaring Fork Motor Nature Trail, road view after stop ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  1. 9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  2. 8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  3. 5. Roaring Fork Motor Nature Trail, vista at stop three. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  4. 1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  5. 3. Roaring Fork Motor Nature Trail, view between second and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  6. 2. Roaring Fork Motor Nature Trail, road view before first ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  7. 7. Roaring Fork Motor Nature Trail, rocks along edge of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  8. Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  9. 12. Roaring Fork Motor Nature Trail, place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  10. 11. Roaring Fork Motor Nature Trail, boulders along road after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  11. 75 FR 54601 - Lower South Fork LLC; Notice of Application Accepted for Filing and Soliciting Comments, Motions...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-08

    .... e. Name of Project: Lower South Fork Irrigation Project. f. Location: The project is located in... Contact: Anthony DeLuca, (202) 502-6632, Anthony.deluca@ferc.gov . j. Status of Environmental Analysis... from one ditch to another within an irrigation system. The primary purpose of the conduit...

  12. Retained garden fork following cranial stab injury

    PubMed Central

    Gonya, Sonwabile; Mbatha, Andile; Moyeni, Nondabula; Enicker, Basil

    2016-01-01

    Retained garden fork is a rare complication of penetrating cranial trauma. Retained knife blade is the most commonly reported presentation. We report an unusual case of a 30-year-old male patient treated at our institution, who presented with a retained garden fork following a stab to the head, with no associated neurological deficits. Computerized tomographic scan of the brain was performed preoperatively to assess the trajectory of the weapon and parenchymal injury. A craniectomy was performed to facilitate removal of the weapon in the operating theatre under general anaesthesia. Intravenous prophylactic antibiotics were administered pre- and postoperatively to prevent septic complications. The patient recovered well and was discharged home. PMID:26747398

  13. Transonic Flows of Bethe-Zel'dovich-Thompson Fluids

    NASA Astrophysics Data System (ADS)

    Cramer, Mark; Andreyev, Aleksandr

    2013-11-01

    We examine steady transonic flows of Bethe-Zel'dovich-Thompson (BZT) fluids over thin turbine blades or airfoils. BZT fluids are ordinary fluids having a region of negative fundamental derivative over a finite range of pressures and temperatures in the single phase regime. We present the transonic small disturbance equation, shock jump conditions, and shock existence conditions capable of capturing the qualitative behavior of BZT fluids. The flux function is seen to be quartic in the pressure or density perturbation rather than the quadratic (convex) flux function of the perfect gas theory. We show how this nonconvex flux function can be used to predict and explain the complex flows possible. Numerical solutions using a successive line relaxation (SLR) scheme are presented. New results of interest include shock-splitting, collisions between expansion and compression shocks, two compressive bow shocks in supersonic flows, and the observation of as many as three normal stern shocks following an oblique trailing edge shock.

  14. Thompson Receives 2013 Early Career Hydrologic Science Award: Citation

    NASA Astrophysics Data System (ADS)

    Katul, Gabriel

    2014-08-01

    Sally Thompson grew up in Perth, where she was trained as an environmental engineer at the University of Western Australia. She graduated with honors in 2003 and worked for a few years as an environmental engineering consultant. Following the award of the Sir John Monash Fellowship in Australia, Sal accepted the admissions offer from the Nicholas School of the Environment at Duke University in 2006, completing her Ph.D. within 4 years and defending her dissertation in 2010. I was most fortunate to have Sal join me at Duke after an enthusiastic recommendation from Siva. Upon her arrival at Duke University, it was immediately clear to all that Sal is a special person with the remarkable skill of being able to identify the main aspects of a problem and throw at them the best that theory, experiment, and modeling tools offer.

  15. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  16. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  17. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  18. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  19. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  20. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES... and to test for vibratory sense. (b) Classification. Class I (general controls). The device is...

  1. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES... is a mechanical device which resonates at a given frequency and is used to diagnose hearing...

  2. Development of the Pintle Release Fork Mechanism

    SciTech Connect

    BOGER, R.M.; DALE, R.

    1999-08-27

    An improved method of attachment of the pintle to the piston in the universal sampler is being developed. The mechanism utilizes a forked release disk which captures two balls in a cavity formed by a hole in the piston and a groove in the pintle rod.

  3. Man-induced gradient adjustment of the South Fork Forked Deer River, west Tennessee

    USGS Publications Warehouse

    Simon, A.; Robbins, C.H.

    1987-01-01

    Channel modifications from 1968 to 1969 on the South Fork Forked Deer River in western Tennessee have caused upstream degradation, downstream aggradation, and bank failures along the altered channels, adjacent reaches, and tributaries. The result of these adjustments is a general decrease in gradient as the channel attempts to absorb the imposed increase in energy conditions created by channelization. Headward degradation at a rate of approximately 2.57 km/yr on the South Fork Forked Deer River caused from 1.52 m to about 3.14 m of incision over a 13.5 km reach from 1969 to 1981. As a consequence of substantially increased sediment supply, approximately 2.13 m of aggradation was induced downstream of this reach during the same period. This accumulation represents a 60% recovery of bed level at the downstream site since the completion of channel work in 1969. Gradient adjustment with time is described by exponential decay functions. The length of time required for adjustment to some new quasi-equilibrium condition is computed by these decay functions and is about 20 years from the completion of channel work. Adjusted slopes are less than predisturbed values, probably because straightened channels dissipate less energy by friction, allowing more energy for sediment transport. An equivalent sediment load, therefore, can be transported at a considerably gentler slope. The predisturbed slope exceeds the adjusted slope by an order of magnitude on the downstream reach of the South Fork Forked Deer River. ?? 1987 Springer-Verlag New York Inc.

  4. Yielding the bully pulpit: a conversation with Tommy Thompson. Interview by John K Iglehart.

    PubMed

    Thompson, Tommy

    2005-01-01

    In January 2005 Tommy Thompson relinquished the post of secretary of the U.S. Department of Human Services (HHS). Known as being an outspoken defender of his principles throughout his tenure at HHS, Thompson continues that tone in an interview conducted shortly after he left Washington. Topics covered include ways to curb Medicare spending; devote greater attention to preventive health and proper incentives for technology; improve the vaccine infrastructure of the United States and the world; and continue to infuse U.S. foreign policy with what Thompson has termed "medical diplomacy," in an effort to counter the messages of America's enemies. PMID:15886146

  5. Wildlife Impact Assessment and Summary of Previous Mitigation Related to Hydroelectric Projects in Montana, Phase 1, Volume Two (B), Clark Fork River Projects, Cabinet Gorge and Noxon Rapids Dams, Operator, Washington Water Power Company.

    SciTech Connect

    Wood, Marilyn

    1984-06-01

    This report documents best available information concerning the wildlife species impacted and the degree of the impact. A target species list was developed to focus the impact assessment and to direct mitigation efforts. Many non-target species also incurred impacts but are not discussed in this report. All wildlife habitats inundated by the two reservoirs are represented by the target species. It was assumed the numerous non-target species also affected will be benefited by the mitigation measures adopted for the target species. Impacts addressed are limited to those directly attributable to the loss of habitat and displacement of wildlife populations due to the construction and operation of the two hydroelectric projects. Secondary impacts, such as the relocation of railroads and highways, and the increase of the human population, were not considered. In some cases, both positive and negative impacts were assessed; and the overall net effect was reported. The loss/gain estimates reported represent impacts considered to have occurred during one point in time except where otherwise noted. When possible, quantitative estimates were developed based on historical information from the area or on data from similar areas. Qualitative loss estimates of low, moderate, or high with supporting rationale were assessed for each species or species group.

  6. The Golden-Thompson inequality: Historical aspects and random matrix applications

    SciTech Connect

    Forrester, Peter J. Thompson, Colin J.

    2014-02-15

    The Golden-Thompson inequality, Tr (e{sup A+B}) ⩽ Tr (e{sup A}e{sup B}) for A, B Hermitian matrices, appeared in independent works by Golden and Thompson published in 1965. Both of these were motivated by considerations in statistical mechanics. In recent years the Golden-Thompson inequality has found applications to random matrix theory. In this article, we detail some historical aspects relating to Thompson's work, giving in particular a hitherto unpublished proof due to Dyson, and correspondence with Pólya. We show too how the 2 × 2 case relates to hyperbolic geometry, and how the original inequality holds true with the trace operation replaced by any unitarily invariant norm. In relation to the random matrix applications, we review its use in the derivation of concentration type lemmas for sums of random matrices due to Ahlswede-Winter, and Oliveira, generalizing various classical results.

  7. The Golden-Thompson inequality: Historical aspects and random matrix applications

    NASA Astrophysics Data System (ADS)

    Forrester, Peter J.; Thompson, Colin J.

    2014-02-01

    The Golden-Thompson inequality, Tr (eA+B) ⩽ Tr (eAeB) for A, B Hermitian matrices, appeared in independent works by Golden and Thompson published in 1965. Both of these were motivated by considerations in statistical mechanics. In recent years the Golden-Thompson inequality has found applications to random matrix theory. In this article, we detail some historical aspects relating to Thompson's work, giving in particular a hitherto unpublished proof due to Dyson, and correspondence with Pólya. We show too how the 2 × 2 case relates to hyperbolic geometry, and how the original inequality holds true with the trace operation replaced by any unitarily invariant norm. In relation to the random matrix applications, we review its use in the derivation of concentration type lemmas for sums of random matrices due to Ahlswede-Winter, and Oliveira, generalizing various classical results.

  8. EMPIRICAL GENERAL POPULATION ASSESSMENT OF THE HORVITZ-THOMPSON ESTIMATOR UNDER VARIABLE PROBABILITY SAMPLING

    EPA Science Inventory

    The variance and two estimators of variance of the Horvitz-Thompson estimator were studied under randomized, variable probability systematic sampling. hree bivariate distributions, representing the populations, were investigated empirically, with each distribution studied for thr...

  9. Dioxin Registry report for Thompson Chemical Company, St. Louis, Missouri

    SciTech Connect

    Marlow, D.A.; Fingerhut, M.A.

    1991-01-01

    An industrial hygiene survey was conducted at the Thompson Chemical Company, St. Louis, Missouri. The company manufactured sodium-2,4,5-trichlorophenate (NaTCP), 2,4,5-trichlorophenoxyacetic-acid (2,4,5-T), and esters and amines of 2,4,5-T from 1949 through 1970. The company manufactured Agent-Orange for the Air Force from 1967 until 1969. The facility, its processes, its workforce, and 2,3,7,8-tetrachlorodibenzo-p-dioxin (1746016) (TCDD) analytical results for these products were examined. As this firm filed for bankruptcy in 1970, there was only limited data available. The facility employed about 12 persons year round and about double this number during the busy season and during the Agent-Orange contract. Almost no information was available about the production processes. Nine persons developed chloracne after an accident and fire in the NaTCP process. Few personnel records were available. The authors conclude that workers employed from 1949 through 1970 are suitable for inclusion into the Dioxin Registry. There is not enough information available to construct a TCDD exposure matrix for this site.

  10. Quartz tuning fork based microwave impedance microscopy

    NASA Astrophysics Data System (ADS)

    Cui, Yong-Tao; Ma, Eric Yue; Shen, Zhi-Xun

    2016-06-01

    Microwave impedance microscopy (MIM), a near-field microwave scanning probe technique, has become a powerful tool to characterize local electrical responses in solid state samples. We present the design of a new type of MIM sensor based on quartz tuning fork and electrochemically etched thin metal wires. Due to a higher aspect ratio tip and integration with tuning fork, such design achieves comparable MIM performance and enables easy self-sensing topography feedback in situations where the conventional optical feedback mechanism is not available, thus is complementary to microfabricated shielded stripline-type probes. The new design also enables stable differential mode MIM detection and multiple-frequency MIM measurements with a single sensor.

  11. The E. coli DNA Replication Fork.

    PubMed

    Lewis, J S; Jergic, S; Dixon, N E

    2016-01-01

    DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds bidirectionally, resulting in two replication forks that travel in opposite directions from the origin. Here, we focus on events at the replication fork. The replication machinery (or replisome), first assembled on both forks at oriC, contains the DnaB helicase for strand separation, and the DNA polymerase III holoenzyme (Pol III HE) for DNA synthesis. DnaB interacts transiently with the DnaG primase for RNA priming on both strands. The Pol III HE is made up of three subassemblies: (i) the αɛθ core polymerase complex that is present in two (or three) copies to simultaneously copy both DNA strands, (ii) the β2 sliding clamp that interacts with the core polymerase to ensure its processivity, and (iii) the seven-subunit clamp loader complex that loads β2 onto primer-template junctions and interacts with the α polymerase subunit of the core and the DnaB helicase to organize the two (or three) core polymerases. Here, we review the structures of the enzymatic components of replisomes, and the protein-protein and protein-DNA interactions that ensure they remain intact while undergoing substantial dynamic changes as they function to copy both the leading and lagging strands simultaneously during coordinated replication. PMID:27241927

  12. X-15 test pilots - Thompson, Dana, and McKay

    NASA Technical Reports Server (NTRS)

    1966-01-01

    NASA pilots Milton O. Thompson, William H. 'Bill' Dana, and John B. 'Jack' McKay are seen here in front of the #2 X-15 (56-6671) rocket-powered research aircraft. Among them, the three NASA research pilots made 59 flights in the X-15 (14 for Thompson, 16 for Dana, and 29 for McKay). The X-15 was a rocket-powered aircraft 50 ft long with a wingspan of 22 ft. It was a missile-shaped vehicle with an unusual wedge-shaped vertical tail, thin stubby wings, and unique fairings that extended along the side of the fuselage. The X-15 weighed about 14,000 lb empty and approximately 34,000 lb at launch. The XLR-99 rocket engine, manufactured by Thiokol Chemical Corp., was pilot controlled and was capable of developing 57,000 lb of rated thrust (actual thrust reportedly climbed to 60,000 lb). North American Aviation built three X-15 aircraft for the program. The X-15 research aircraft was developed to provide in-flight information and data on aerodynamics, structures, flight controls, and the physiological aspects of high-speed, high-altitude flight. A follow-on program used the aircraft as a testbed to carry various scientific experiments beyond the Earth's atmosphere on a repeated basis. For flight in the dense air of the usable atmosphere, the X-15 used conventional aerodynamic controls such as rudder surfaces on the vertical stabilizers to control yaw and canted horizontal surfaces on the tail to control pitch when moving in synchronization or roll when moved differentially. For flight in the thin air outside of the appreciable Earth's atmosphere, the X-15 used a reaction control system. Hydrogen peroxide thrust rockets located on the nose of the aircraft provided pitch and yaw control. Those on the wings provided roll control. Because of the large fuel consumption, the X-15 was air launched from a B-52 aircraft at 45,000 ft and a speed of about 500 mph. Depending on the mission, the rocket engine provided thrust for the first 80 to 120 sec of flight. The remainder of the

  13. Optimal control of information epidemics modeled as Maki Thompson rumors

    NASA Astrophysics Data System (ADS)

    Kandhway, Kundan; Kuri, Joy

    2014-12-01

    We model the spread of information in a homogeneously mixed population using the Maki Thompson rumor model. We formulate an optimal control problem, from the perspective of single campaigner, to maximize the spread of information when the campaign budget is fixed. Control signals, such as advertising in the mass media, attempt to convert ignorants and stiflers into spreaders. We show the existence of a solution to the optimal control problem when the campaigning incurs non-linear costs under the isoperimetric budget constraint. The solution employs Pontryagin's Minimum Principle and a modified version of forward backward sweep technique for numerical computation to accommodate the isoperimetric budget constraint. The techniques developed in this paper are general and can be applied to similar optimal control problems in other areas. We have allowed the spreading rate of the information epidemic to vary over the campaign duration to model practical situations when the interest level of the population in the subject of the campaign changes with time. The shape of the optimal control signal is studied for different model parameters and spreading rate profiles. We have also studied the variation of the optimal campaigning costs with respect to various model parameters. Results indicate that, for some model parameters, significant improvements can be achieved by the optimal strategy compared to the static control strategy. The static strategy respects the same budget constraint as the optimal strategy and has a constant value throughout the campaign horizon. This work finds application in election and social awareness campaigns, product advertising, movie promotion and crowdfunding campaigns.

  14. BALD ROCK AND MIDDLE FORK FEATHER RIVER ROADLESS AREAS, CALIFORNIA.

    USGS Publications Warehouse

    Sorensen, Martin L.; Buehler, Alan R.

    1984-01-01

    The results of a mineral-resource assessment of the Bald Rock and Middle Fork Feather River Roadless Areas in California indicate several areas within the Middle Fork Feather River Roadless Area that have probable mineral-resource potential. A probable potential for placer gold exists at various localities, both in areas covered by Tertiary volcanic rocks and in small streams that drain into the Middle Fork of the Feather River. A probable potential for small deposits of chromite exists in tracts underlain by ultramafic rocks in the Melones fault zone. A probable potential for lead-silver deposits is recognized at the east end of the Middle Fork Feather River Roadless Area.

  15. Evolution of tail fork depth in genus Hirundo.

    PubMed

    Hasegawa, Masaru; Arai, Emi; Kutsukake, Nobuyuki

    2016-02-01

    A classic example of a sexually selected trait, the deep fork tail of the barn swallow Hirundo rustica is now claimed to have evolved and be maintained mainly via aerodynamic advantage rather than sexually selected advantage. However, this aerodynamic advantage hypothesis does not clarify which flight habits select for/against deep fork tails, causing diversity of tail fork depth in hirundines. Here, by focusing on the genus Hirundo, we investigated whether the large variation in tail fork depth could be explained by the differential flight habits. Using a phylogenetic comparative approach, we found that migrant species had deeper fork tails, but less colorful plumage, than the other species, indicating that migration favors a specific trait, deep fork tails. At the same time, tail fork depth but not plumage coloration decreased with increasing bill size - a proxy of prey size, suggesting that foraging on larger prey items favors shallower fork tails. Variation of tail fork depth in the genus Hirundo may be explained by differential flight habits, even without assuming sexual selection. PMID:26865972

  16. A description of the hydrologic system and the effects of coal mining on water quality in the East Fork Little Chariton River and the alluvial aquifer between Macon and Huntsville, north-central Missouri

    USGS Publications Warehouse

    Hall, D.C.

    1986-01-01

    The quality of surface and groundwater has been affected by abandoned strip mines and by abandoned underground mines in a 110-sq mi subbasin of the East Fork Little Chariton River. More than 14% of the area was strip mined for coal before 1979. The hydrologic system in the area was investigated and the effects of coal mining on quality of water in the river and alluvial aquifer were analyzed, with major emphasis on defining strip-mining effects. The groundwater gradient was from glacial drift or coal spring to alluvium to the East Fork Little Chariton River, and was greatest in spring and least in fall. Seepage from alluvium to the East Fork Little Chariton River occurs throughout the year, except during drought conditions when the only river flow is water released from Long Branch Lake. In the East Fork Little Chariton River median dissolved-solids concentrations increased from 153 mg/L near Macon to 630 mg/L near Huntsville and median sulfate concentrations increased from 36 mg/L near Macon to 360 mg/L near Huntsville. The median dissolved-solids concentration in water from the alluvium increased from 408 mg/L upstream from the strip mines to 641 mg/L near the mines and median dissolved-sulfate concentration increased from 140 to 350 mg/L. The sulfate-to-chloride ratio, used as the most sensitive indicator of strip-mining effects, increased markedly downstream in the East Fork Little Chariton River and nearby Middle Fork Little Chariton River, which also is affected by strip mining. There were no significant increases in sulfate-to-chloride ratio and dissolved-solids concentrations in comparable nearby subbasins of the Grand, Thompson, and Chariton Rivers where there was no mining. (Author 's abstract)

  17. The Australian Monstrilloida (Crustacea: Copepoda) II. Cymbasoma Thompson, 1888.

    PubMed

    Suárez-Morales, Eduardo; Mckinnon, A David

    2016-01-01

    Monstrilloid copepods collected during the past two decades from zooplankton surveys in reef and coastal areas of Australia were analyzed. A first contribution included the taxonomic analysis of three genera of the Monstrilloida, Monstrillopsis Sars, 1921, Maemonstrilla Grygier & Ohtsuka, 2008, and the newly described Australomonstrillopsis Suárez-Morales & McKinnon, 2014. In this document a taxonomic analysis of the species belonging to the genus Cymbasoma Thompson, 1888 is provided. A total of 28 species were found, most of them being undescribed. Seventeen species were described based on females only and eight on male specimens while three species were described from both sexes. Males of Australian species of Cymbasoma are distinguished by details of the genital complex, body size and proportions, ornamentation and processes of the cephalic region, number of caudal setae, and the characteristic structure or ornamentation of the genital lappets. Two main groups of males were distinguished on the basis of the number of caudal setae (3 or 4). As for the females, 20 of the 25 new species of Cymbasoma have fifth legs with an unarmed inner lobe and three setae on the outer lobe; one of these species (C. jinigudira sp. nov.) belongs to the C. longispinosum species-group (sensu Üstün et al. 2014). Another group, consisting of five species, has only two setae on the outer (exopodal) lobe. There were no Australian species of Cymbasoma with a single lobe. A species group, named after C. agoense, is proposed to include species sharing a globose body and a female fifth leg with a large endopodal lobe and an outer (exopodal) lobe with two setae. The females of the new species of Cymbasoma from Australia can be distinguished from their known congeners by unique combinations of characters including the type of body ornamentation, body size and shape, antennule armature and proportions, the presence of distinctive features of the legs 1-4, the presence/absence of processes on

  18. Surface-water quality assessment of the North Fork Red River basin upstream from Lake Altus, Oklahoma, 2002

    USGS Publications Warehouse

    Smith, S. Jerrod; Schneider, M.L.; Masoner, J.R.; Blazs, R.L.

    2003-01-01

    Elevated salinity in the North Fork Red River is a major concern of the Bureau of Reclamation W. C. Austin Project at Lake Altus. Understanding the relation between surface-water runoff, ground-water discharge, and surface-water quality is important for maintaining the beneficial use of water in the North Fork Red River basin. Agricultural practices, petroleum production, and natural dissolution of salt-bearing bedrock have the potential to influence the quality of nearby surface water. The U.S. Geological Survey, in cooperation with the Bureau of Reclamation, sampled stream discharge and water chemistry at 19 stations on the North Fork Red River and tributaries. To characterize surface-water resources of the basin in a systematic manner, samples were collected synoptically during receding streamflow conditions during July 8-11, 2002. Together, sulfate and chloride usually constitute greater than half of the dissolved solids. Concentrations of sulfate ranged from 87.1 to 3,450 milligrams per liter. The minimum value was measured at McClellan Creek near Back (07301220), and the maximum value was measured at Bronco Creek near Twitty (07301303). Concentrations of chloride ranged from 33.2 to 786 milligrams per liter. The minimum value was measured at a North Fork Red River tributary (unnamed) near Twitty (07301310), and the maximum value was measured at the North Fork Red River near Back (07301190), the most upstream sample station.

  19. 76 FR 29721 - Lost River and Challis-Yankee Fork Ranger Districts, Salmon-Challis National Forest; ID; Lost...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-23

    ... National Forest System lands managed by the Salmon-Challis National Forest. This project has been cancelled... Forest Service Lost River and Challis-Yankee Fork Ranger Districts, Salmon- Challis National Forest; ID... National Forest, 1206 S. Challis St., Salmon, ID 83467. Dated: May 6, 2011. Frank V. Guzman,......

  20. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED, GEORGIA

    EPA Science Inventory

    There is an urgent need for EPA to develop protocols for establishing Total Maximum Daily Loads (TMDLs) in streams, lakes and estuaries. A cooperative TMDL field data collection project between ORD and Region 4 is ongoing in the South Fork Broad River Watershed (SFBR), a 245.18 ...

  1. Community Learning Campus: It Takes a Simple Message to Build a Complex Project

    ERIC Educational Resources Information Center

    Pearson, George

    2012-01-01

    Education Canada asked Tom Thompson, president of Olds College and a prime mover behind the Community Learning Campus (CLC): What were the lessons learned from this unusually ambitious education project? Thompson mentions six lessons he learned from this complex project which include: (1) Dream big, build small, act now; (2) Keep a low profile at…

  2. A fork-clearing role for UvrD.

    PubMed

    Florés, Maria-José; Sanchez, Nicolas; Michel, Bénédicte

    2005-09-01

    The inactivation of a replication protein causes the disassembly of the replication machinery and creates a need for replication reactivation. In several replication mutants, restart occurs after the fork has been isomerized into a four-armed junction, a reaction called replication fork reversal. The repair helicase UvrD is essential for replication fork reversal upon inactivation of the polymerase (DnaE) or the beta-clamp (DnaN) subunits of the Escherichia coli polymerase III, and for the viability of dnaEts and dnaNts mutants at semi-permissive temperature. We show here that the inactivation of recA, recFOR, recJ or recQ recombination genes suppresses the requirement for UvrD for replication fork reversal and suppresses the lethality conferred by uvrD inactivation to Pol IIIts mutants at semi-permissive temperature. We propose that RecA binds inappropriately to blocked replication forks in the dnaEts and dnaNts mutants in a RecQ- RecJ- RecFOR-dependent way and that UvrD acts by removing RecA or a RecA-made structure, allowing replication fork reversal. This work thus reveals the existence of a futile reaction of RecA binding to blocked replication forks, that requires the action of UvrD for fork-clearing and proper replication restart. PMID:16135232

  3. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Big Fork River, Minn.; logging..., DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the season... maintained at all times for the navigation of steamboats, flatboats, and other small craft. (b) A...

  4. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Big Fork River, Minn.; logging..., DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the season... maintained at all times for the navigation of steamboats, flatboats, and other small craft. (b) A...

  5. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Big Fork River, Minn.; logging..., DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the season... maintained at all times for the navigation of steamboats, flatboats, and other small craft. (b) A...

  6. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Big Fork River, Minn.; logging..., DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the season... maintained at all times for the navigation of steamboats, flatboats, and other small craft. (b) A...

  7. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Big Fork River, Minn.; logging..., DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the season... maintained at all times for the navigation of steamboats, flatboats, and other small craft. (b) A...

  8. South Fork Telephone Switchboard Building, general view in setting showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, general view in setting showing (N) side; view (S) - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  9. South Fork Latrine, interior showing head with steel tank mounted ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, interior showing head with steel tank mounted to wall; view south - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  10. South Fork Telephone Switchboard Building, interior west room showing hardwood ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, interior west room showing hardwood floor; view south - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  11. South Fork Telephone Switchboard Building, oblique view of (W) and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, oblique view of (W) and (S) sides, view to northeast - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  12. South Fork Telephone Switchboard Building, oblique view of east side; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, oblique view of east side; view northwest - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  13. 14. Roaring Fork Motor Nature Trail, Place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  14. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Roanoke. 9.65 Section 9.65 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL AMERICAN VITICULTURAL AREAS Approved American Viticultural Areas § 9.65 North Fork of Roanoke. (a) Name. The name...

  15. Design of a tuning-fork gyro made of quartz

    NASA Astrophysics Data System (ADS)

    Jia, Yubin; Sun, Yunan; Qin, BingKun; Cui, Fang; Chen, Gang

    1998-08-01

    Based on piezoelectric effect of quartz, a design of tuning- fork gyroscopes made of quartz was presented in this paper. The gyroscope is a kind of micro-machined quartz angular rate sensor. Its structure is similar to a tuning fork in quartz watch. In the gyroscope, the piezoelectric effect in quartz is used both to excite a reference vibration in the plane of tuning fork and to detect a vibration normal to this plane due to an externally applied rotation. The amplitude of the second vibration is directly proportional to the angular velocity of the applied rotation. In contrast to ordinary types of tuning fork gyroscopes, this gyroscope uses a single piece of quartz, the sensor element is of a design in which the only vibrationally, active areas are the tines of the tuning fork.

  16. Inside the Thompson laboratory during the "cerebellar years" and the continuing cerebellar story.

    PubMed

    Lavond, D G; Wikgren, J; Nokia, M S

    2011-02-01

    This paper is based on the talk by one of the authors (DL) given at the symposium for the retirement of RF Thompson (RF Thompson: A bridge between 20th and 21st century neuroscience). We first make some informal observations of the historical times and research conditions in the Thompson laboratory when the cerebellum was found to play a critical role in eye lid classical conditioning, the "cerebellar years". These conditions influenced our collaborative international program on the phenomenon known as "transfer of training" or "savings". Our research shows that the appearance of "savings" is an artifact of the order of testing, and depends upon the functioning of the contralateral interpositus nucleus (IPN) in a way that is complementary to the role of the IPN in normal eyelid classical conditioning. PMID:21073973

  17. Test pilots 1962 - Armstrong, Walker, Dana, Peterson, McKay, Thompson, Butchart

    NASA Technical Reports Server (NTRS)

    1962-01-01

    The research pilots at what in 1962 was called the Flight Research Center standing in front of the X-1E. They are (left to right) Neil Armstrong, Joe Walker, Bill Dana, Bruce Peterson, Jack McKay, Milt Thompson, and Stan Butchart. of the group, Armstrong, Walker, Dana, McKay and Thompson all flew the X-15. Bruce Peterson flew the M2-F2 and HL-10 lifting bodies, while Stan Butchart was the B-29 drop plane pilot for many of the D-558-II and X-1 series research aircraft.

  18. As-Built documentation of programs to implement the Robertson and Doraiswamy/Thompson models

    NASA Technical Reports Server (NTRS)

    Valenziano, D. J. (Principal Investigator)

    1981-01-01

    The software which implements two spring wheat phenology models is described. The main program routines for the Doraiswamy/Thompson crop phenology model and the basic Robertson crop phenology model are DTMAIN and BRMAIN. These routines read meteorological data files and coefficient files, accept the planting date information and other information from the user, and initiate processing. Daily processing for the basic Robertson program consists only of calculation of the basic Robertson increment of crop development. Additional processing in the Doraiswamy/Thompson program includes the calculation of a moisture stress index and correction of the basic increment of development. Output for both consists of listings of the daily results.

  19. M2-F1 on lakebed with pilots Milt Thompson, Chuck Yeager, Don Mallick, and Bruce Peterson

    NASA Technical Reports Server (NTRS)

    1963-01-01

    After the initial M2-F1 airtow flights, the NASA Flight Research Center used the vehicle to check out other pilots. Bruce Peterson was scheduled to take over as the M2-F1 project pilot from Milt Thompson, while Don Mallick was to be his backup. Col. (later Brig. Gen.) Charles (Chuck) Yeager, then commandant of the Air Force's Aerospace Research Pilots School, wanted to evaluate a possible lifting-body trainer for the school. This photo shows all of these distinguished pilots on or in the M2-F1, with Col. Yeager in the pilot's seat. The lifting body concept evolved in the mid-1950s as researchers considered alternatives to ballistic reentries of piloted space capsules. The designs for hypersonic, wingless vehicles were on the boards at NASA Ames and NASA Langley facilities, while the US Air Force was gearing up for its Dyna-Soar program, which defined the need for a spacecraft that would land like an airplane. Despite favorable research on lifting bodies, there was little support for a flight program. Dryden engineer R. Dale Reed was intrigued with the lifting body concept, and reasoned that some sort of flight demonstration was needed before wingless aircraft could be taken seriously. In February 1962, he built a model lifting body based upon the Ames M2 design, and air-launched it from a radio controlled 'mothership.' Home movies of these flights, plus the support of research pilot Milt Thompson, helped pursuade the facilities director, Paul Bikle, to give the go-ahead for the construction of a full-scale version, to be used as a wind-tunnel model and possibly flown as a glider. Comparing lifting bodies to space capsules, an unofficial motto of the project was, 'Don't be Rescued from Outer Space--Fly Back in Style.' The construction of the M2-F1 was a joint effort by Dryden and a local glider manufacturer, the Briegleb Glider Company. The budget was $30,000. NASA craftsmen and engineers built the tubular steel interior frame. Its mahogany plywood shell was hand

  20. Late Holocene environmental changes recorded in the sediments of Lago Thompson, Northern Chilean Patagonia

    NASA Astrophysics Data System (ADS)

    Fagel, Nathalie; Nuttin, Laurence; Bertrand, Sébastien; Borgniet, Geoffrey; Schmidt, Sabine; Araneda, Alberto; Torrejon, Fernando; Urrutia, Roberto

    2010-05-01

    We investigate the sedimentary record of Lago Thompson, a small lake (area 1.1 km2, watershed ~8 km2) located at an elevation of 750 masl in Northern Chilean Patagonia (45.38 °S, 71.47°W). At Present, the climate conditions around the lake are relatively dry, with annual precipitation averaging 500 mm. The goal of this study is to evaluate the natural climate variability over the Last Millennium in Northern Chilean Patagonia and its impact on the environment. The coring site was selected after a bathymetric survey using an echo-sounder. Several short cores were retrieved in 2008 using an Uwitec gravity corer: the length of the cores ranges between 124 and 132 cm. The preliminary age model is based on 4 AMS radiocarbon ages measured on bulk sediment and organic macro-remains. The radiocarbon ages demonstrate that the sediment cores cover the last 850 to 950 years. This sediment record, which is characterized by accumulation rates ranging between 1 and 3 mm/yr, can therefore be studied at decennial to centennial resolution. To reconstruct past changes in climate and the environment, we conducted a multiproxy study combining sedimentological, mineralogical and geochemical analyses (LOI 105, 550 and 950°C, biogenic silica content, magnetic susceptibility, tephrostratigraphy, bulk organic geochemistry, bulk mineralogy by X-ray diffraction, and inorganic geochemistry by ITRAX core scanner and ICP-AES). Visual descriptions and X-ray radiographies demonstrate that the sediment record is relatively undisturbed. The sediment is composed of light brown organic-rich clayey material. The lower part of the core (below 75 cm) is finely laminated whereas the upper part is more homogeneous. Magnetic susceptibility values highlight two tephra layers that represent explosive eruptions of volcanoes from the southern volcanic zone of Chile (Hudson, Cay, or Macá volcanoes). The biogenic silica content of the sediment retrieved in Lago Thompson is high (40 to 80%), and probably

  1. Building on Our Knowledge of Racism, Mental Health, and Mental Health Practice: A Reaction to Thompson and Neville.

    ERIC Educational Resources Information Center

    D'Andrea, Michael; Daniels, Judy

    1999-01-01

    Presents a reaction to Thompson and Neville's (1999) article, "Racism, Mental Health, and Mental Health Practice." Discusses the results of their own longitudinal study of the psychology of White racism to both promote and validate many of the theoretical claims that are presented in Thompson and Neville's article. (GCP)

  2. Complete genomic sequences of two outbreak strains of Salmonella enterica subsp. enterica serovar Thompson associated with cilantro

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Salmonella enterica subsp. enterica serovar Thompson strains RM1984 (CADPH-99A2334) and RM1986 (CADPH -99A2345) are clinical isolates from 1999, putatively related to an outbreak in California from contaminated cilantro. We report the complete genome sequences and annotation of these two S. Thompson...

  3. Complete Genome Sequences of Two Outbreak Strains of Salmonella enterica subsp. enterica Serovar Thompson Associated with Cilantro

    PubMed Central

    Huynh, Steven; Gorski, Lisa; Cooper, Kerry K.; Miller, William G.

    2015-01-01

    Salmonella enterica subsp. enterica serovar Thompson strains RM1984 (CADPH-99A2334) and RM1986 (CADPH-99A2345) are associated with a 1999 outbreak in contaminated cilantro. We report here the complete genome sequences and annotation of these two S. Thompson strains. These genomes are distinct and provide additional data for our understanding of S. enterica. PMID:26586897

  4. Complete Genome Sequences of Two Outbreak Strains of Salmonella enterica subsp. enterica Serovar Thompson Associated with Cilantro.

    PubMed

    Parker, Craig T; Huynh, Steven; Gorski, Lisa; Cooper, Kerry K; Miller, William G

    2015-01-01

    Salmonella enterica subsp. enterica serovar Thompson strains RM1984 (CADPH-99A2334) and RM1986 (CADPH-99A2345) are associated with a 1999 outbreak in contaminated cilantro. We report here the complete genome sequences and annotation of these two S. Thompson strains. These genomes are distinct and provide additional data for our understanding of S. enterica. PMID:26586897

  5. Complete Genomic Sequences of Two Outbreak Strains of Salmonella enterica subsp. enterica serovar Thompson Associated with Cilantro

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Salmonella enterica subsp. enterica serovar Thompson strains RM1984 (CADPH-99A2334) and RM1986 (CADPH -99A2345) are clinical isolates from 1999, putatively related to an outbreak in California from contaminated cilantro. We report the complete genome sequences and annotation of these two S. Thompson...

  6. Fork gratings based on ferroelectric liquid crystals.

    PubMed

    Ma, Y; Wei, B Y; Shi, L Y; Srivastava, A K; Chigrinov, V G; Kwok, H-S; Hu, W; Lu, Y Q

    2016-03-21

    In this article, we disclose a fork grating (FG) based on the photo-aligned ferroelectric liquid crystal (FLC). The Digital Micro-mirror Device based system is used as a dynamic photomask to generated different holograms. Because of controlled anchoring energy, the photo alignment process offers optimal conditions for the multi-domain FLC alignment. Two different electro-optical modes namely DIFF/TRANS and DIFF/OFF switchable modes have been proposed where the diffraction can be switched either to no diffraction or to a completely black state, respectively. The FLC FG shows high diffraction efficiency and fast response time of 50µs that is relatively faster than existing technologies. Thus, the FLC FG may pave a good foundation toward optical vertices generation and manipulation that could find applications in a variety of devices. PMID:27136779

  7. Chemical and biological sensing using tuning forks

    DOEpatents

    Tao, Nongjian; Boussaad, Salah

    2012-07-10

    A device for sensing a chemical analyte is disclosed. The device is comprised of a vibrating structure having first and second surfaces and having an associated resonant frequency and a wire coupled between the first and second surfaces of the vibrating structure, wherein the analyte interacts with the wire and causes a change in the resonant frequency of the vibrating structure. The vibrating structure can include a tuning fork. The vibrating structure can be comprised of quartz. The wire can be comprised of polymer. A plurality of vibrating structures are arranged in an array to increase confidence by promoting a redundancy of measurement or to detect a plurality of chemical analytes. A method of making a device for sensing a chemical analyte is also disclosed.

  8. Wireless tuning fork gyroscope for biomedical applications

    NASA Astrophysics Data System (ADS)

    Abraham, Jose K.; Varadan, Vijay K.; Whitchurch, Ashwin K.; Sarukesi, K.

    2003-07-01

    This paper presents the development of a Bluetooth enabled wireless tuning fork gyroscope for the biomedical applications, including gait phase detection system, human motion analysis and physical therapy. This gyroscope is capable of measuring rotation rates between -90 and 90 and it can read the rotation information using a computer. Currently, the information from a gyroscope can trigger automobile airbag deployment during rollover, improve the accuracy and reliability of GPS navigation systems and stabilize moving platforms such as automobiles, airplanes, robots, antennas, and industrial equipment. Adding wireless capability to the existing gyroscope could help to expand its applications in many areas particularly in biomedical applications, where a continuous patient monitoring is quite difficult. This wireless system provides information on several aspects of activities of patients for real-time monitoring in hospitals.

  9. Bull Trout Population and Habitat Surveys in the Middle Fork Willamette and McKenzie Rivers, Annual Report 2002.

    SciTech Connect

    Seals, Jason; Reis, Kelly

    2003-10-01

    Bull trout in the Willamette River Basin were historically distributed throughout major tributaries including the Middle Fork Willamette and McKenzie rivers. Habitat degradation, over-harvest, passage barriers, fish removal by rotenone, and hybridization and competition with non-native brook trout are all likely factors that have led to the decline of bull trout in the Willamette Basin (Ratliff and Howell 1992). The U.S. Fish and Wildlife Service listed the Columbia River bull trout population segment as Threatened under the federal Endangered Species Act in 1998. Four bull trout populations were isolated in the upper Willamette River following the construction of flood control dams on the South Fork McKenzie River, McKenzie River, and Middle Fork Willamette River that created Cougar, Trail Bridge, and Hills Creek reservoirs. Buchanan et al. (1997) described the population in the main stem McKenzie as 'of special concern', the South Fork McKenzie population as 'high risk of extinction', the population above Trail Bridge Reservoir as 'high risk of extinction', and bull trout in the Middle Fork Willamette as 'probably extinct'. Various management efforts such as strict angling regulations and passage improvement projects have been implemented to stabilize and rehabilitate bull trout habitat and populations in the McKenzie River over the past 10 years. Since 1997, bull trout fry from Anderson Creek on the upper McKenzie River have been transferred to the Middle Fork Willamette basin above Hills Creek Reservoir in an attempt to re-establish a reproducing bull trout population. This project was developed in response to concerns over the population status and management of bull trout in the McKenzie and Middle Fork Willamette Rivers by the Oregon Department of Fish and Wildlife during the early 1990s. The project was conducted under measure 9.3G(2) of the Columbia Basin Fish and Wildlife Program to monitor the status, life history, habitat needs, and limiting factors for

  10. NORTH FORK JOHN DAY RIVER ROADLESS AREA, OREGON.

    USGS Publications Warehouse

    Evans, James G.; Conyac, Martin D.

    1984-01-01

    A mineral survey of the North Fork John Day River Roadless Area in Oregon indicates that a narrow belt along the river has a substantiated resource potential for placer gold, and several other drainages tributary to the North Fork a probable resource potential for placer or lode gold. Further study of the roadless area may reveal other areas with a potential for gold, and could help to delineate bedrock or additional placer resources, especially in drainages tributary to the North Fork. This work could also point to other mineral deposits near the roadless boundary.

  11. 75 FR 33741 - Safety Zone; Tracey/Thompson Wedding, Lake Erie, Catawba Island, OH

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-15

    ... FR 3316). Public Meeting We do not now plan to hold a public meeting. But you may submit a request... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Tracey/Thompson Wedding, Lake Erie, Catawba... proposes establishing a temporary safety zone on Lake Erie, Catawba Island, Ohio. This temporary...

  12. Religion, Idealism, and African American Autobiography in the Northern Plains: Era Bell Thompson's "American Daughter"

    ERIC Educational Resources Information Center

    Cole, Kevin; Weins, Leah

    2003-01-01

    Era Bell Thompson's "American Daughter", published in 1946 by the University of Chicago Press, is an autobiographical account of an African American woman who comes of age on the plains of North Dakota in the early twentieth century. It deserves to read and included in Great Plains studies because it recounts one of the rarest of American…

  13. Mary Ritter Beard and Marion Thompson Wright: Shaping Inclusive Social Education.

    ERIC Educational Resources Information Center

    Crocco, Margaret Smith

    1997-01-01

    Examines contributions by Mary Ritter Beard and Marion Thompson Wright to inclusive social education curricula. Beard established the field of women's history; Wright promoted the application of black history. Both saw social betterment as the goal of knowledge and sought inclusive understanding of the nature of legitimate knowledge in schools.…

  14. 5. PILOTS KNIGHT, RUSHWORTH, ENGLE, THOMPSON, DANA, AND McKAY STANDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. PILOTS KNIGHT, RUSHWORTH, ENGLE, THOMPSON, DANA, AND McKAY STANDING AT THE NOSE OF X-15 NO. 66671. - Edwards Air Force Base, X-15 Engine Test Complex, Rogers Dry Lake, east of runway between North Base & South Base, Boron, Kern County, CA

  15. "This Strange White World": Race and Place in Era Bell Thompson's "American Daughter"

    ERIC Educational Resources Information Center

    Johnson, Michael K.

    2004-01-01

    Aboard a train heading out of Minneapolis toward frontier North Dakota, Era Bell Thompson in her autobiography "American Daughter" (1946) describes a landscape that grows steadily bleaker with each mile farther west: "Suddenly there was snow--miles and miles of dull, white snow, stretching out to meet the heavy, gray sky; deep banks of snow…

  16. CARBON TRACE GASES IN LAKE AND BEAVER POND ICE NEAR THOMPSON, MANITOBA, CANADA

    EPA Science Inventory

    Concentrations of CO2, CO, and CH4 were measured in beaver pond and lake ice in April 1996 near Thompson, Manitoba to derive information on possible impacts of ice melting on corresponding atmospheric trace gas concentrations. CH4 concentrations in beaver pond and lake ice ranged...

  17. 117. Laurel Fork Viaduct. Elevation view of this 545 1939 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    117. Laurel Fork Viaduct. Elevation view of this 545 1939 steel girder viaduct. Example of structure with plain reinforced concrete arches. Looking northwest. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  18. 35. CHARGING DOOR OF CUPOLA FORM LOFT, WITH FORKS FOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    35. CHARGING DOOR OF CUPOLA FORM LOFT, WITH FORKS FOR FEEDING COKE, FOUNDRY BELOW-LOOKING NORTH. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

  19. TRAIP regulates replication fork recovery and progression via PCNA

    PubMed Central

    Feng, Wanjuan; Guo, Yingying; Huang, Jun; Deng, Yiqun; Zang, Jianye; Huen, Michael Shing-Yan

    2016-01-01

    PCNA is a central scaffold that coordinately assembles replication and repair machineries at DNA replication forks for faithful genome duplication. Here, we describe TRAIP (RNF206) as a novel PCNA-interacting factor that has important roles during mammalian replicative stress responses. We show that TRAIP encodes a nucleolar protein that migrates to stalled replication forks, and that this is accomplished by its targeting of PCNA via an evolutionarily conserved PIP box on its C terminus. Accordingly, inactivation of TRAIP or its interaction with the PCNA clamp compromised replication fork recovery and progression, and leads to chromosome instability. Together, our findings establish TRAIP as a component of the mammalian replicative stress response network, and implicate the TRAIP-PCNA axis in recovery of stalled replication forks. PMID:27462463

  20. 8. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH OF LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING N. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  1. 9. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH OF LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING W. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  2. Little Known Facts about the Common Tuning Fork.

    ERIC Educational Resources Information Center

    Ong, P. P.

    2002-01-01

    Explains the physical principles of the tuning fork which has a common use in teaching laboratories. Includes information on its vibration, frequency of vibration, elasticity, and reasons for having two prongs. (YDS)

  3. 17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK THAT MIXED COFFEE BEANS AS THEY WERE HUSKED - Hacienda Cafetalera Santa Clara, Coffee Mill, KM 19, PR Route 372, Hacienda La Juanita, Yauco Municipio, PR

  4. HOMESTEAD, LAKE FORK, AND LICK CREEK ROADLESS AREAS, OREGON.

    USGS Publications Warehouse

    Evans, James G.; Conyac, Martin D.

    1984-01-01

    A mineral survey concluded that the Homestead, Lake Fork and Lick Creek Roadless Area, Oregon offer little promise for the occurrence of mineral or energy resources in the bedrock. Probable mineral-resource potential is assigned to the west and north parts of the Lake Fork Roadless Area, where gold resources may occur in glacial deposits and alluvium transported into this area from sources outside the roadless area to the west.

  5. Unwinding of forked DNA structures by UvrD.

    PubMed

    Cadman, Chris J; Matson, Steven W; McGlynn, Peter

    2006-09-01

    Many studies have demonstrated the need for processing of blocked replication forks to underpin genome duplication. UvrD helicase in Escherichia coli has been implicated in the processing of damaged replication forks, or the recombination intermediates formed from damaged forks. Here we show that UvrD can unwind forked DNA structures, in part due to the ability of UvrD to initiate unwinding from discontinuities within the phosphodiester backbone of DNA. UvrD does therefore have the capacity to target DNA intermediates of replication and recombination. Such an activity resulted in unwinding of what would be the parental duplex DNA ahead of either a stalled replication fork or a D-loop formed by recombination. However, UvrD had a substrate preference for fork structures having a nascent lagging strand at the branch point but no leading strand. Furthermore, at such structures the polarity of UvrD altered so that unwinding of the lagging strand predominated. This reaction is reminiscent of the PriC-Rep pathway of replication restart, suggesting that UvrD and Rep may have at least partially redundant functions. PMID:16890954

  6. Non-replicative helicases at the replication fork.

    PubMed

    Heller, Ryan C; Marians, Kenneth J

    2007-07-01

    Reactivation of stalled or collapsed replication forks is an essential process in bacteria. Restart systems operate to restore the 5'-->3' replicative helicase, DnaB, to the lagging-strand template. However, other non-replicative 3'-->5' helicases play an important role in the restart process as well. Here we examine the DNA-binding specificity of three of the latter group, PriA, Rep, and UvrD. Only PriA and Rep display structure-specific fork binding. Interestingly, their specificity is opposite: PriA binds a leading-strand fork, presumably reflecting its restart activity in directing loading of DnaB to the lagging-strand template. Rep binds a lagging-strand fork, presumably reflecting its role in partially displacing Okazaki fragments that originate near the fork junction. This activity is necessary for generating a single-stranded landing pad for DnaB. While UvrD shows little structure-specificity, there is a slight preference for lagging-strand forks, suggesting that there might be some redundancy between Rep and UvrD and possibly explaining the observed synthetic lethality that occurs when mutations in the genes encoding these two proteins are combined. PMID:17382604

  7. Force regulated dynamics of RPA on a DNA fork

    PubMed Central

    Kemmerich, Felix E.; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

    2016-01-01

    Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg2+ concentrations, such that human RPA can melt DNA in absence of force. PMID:27016742

  8. Gas Phase Photoacoustic Spectroscopy in the long-wave IR using Quartz Tuning Forks and Amplitude Modulated Quantum Cascade Lasers

    SciTech Connect

    Wojcik, Michael D.; Phillips, Mark C.; Cannon, Bret D.

    2006-12-31

    A paper to accompany a 20 minute talk about the progress of a DARPA funded project called LPAS. ABSTRACT: We demonstrate the performance of a novel long-wave infrared photoacoustic laser absorbance spectrometer for gas-phase species using an amplitude modulated (AM) quantum cascade (QC) laser and a quartz tuning fork microphone. Photoacoustic signal was generated by focusing the output of a Fabry-Perot QC laser operating at 8.41 micron between the legs of a quartz tuning fork which served as a transducer for the transient acoustic pressure wave. The QC laser was modulated at the resonant frequency of the tuning fork (32.8 kHz). This sensor was calibrated using the infrared absorber Freon-134a by performing a simultanious absorption measurement using a 35 cm absorption cell. The NEAS of this instrument was determined to be 2 x 10^-8 W cm^-1 /Hz^1/2 and the fundamental sensitivity of this technique is limited by the noise floor of the tuning fork itself.

  9. Francis Thompson (1859-1907): a medical truant and his troubled heart.

    PubMed

    Breathnach, Caoimhghin S

    2008-02-01

    Francis Thompson was born in 1859 in Preston and grew to manhood in Ashton-under-Lyne in Lancashire. After six years registered as a medical student at Owens College, Manchester, he set off for London to retrace the footsteps of Thomas De Quincey (1785-1859). His early experience in London followed closely that of the earlier English Opium Eater until he was rescued by Wilfrid Meynell (1852-1948) who recognized his nascent literary flair. Thompson's poetry earned him respect and reputation and his prose brought him a reasonable income, but he never weaned himself from laudanum and he died of tuberculosis in 1907. Not every truant is honoured by a lapidary inscription in his alma mater, even though he may be overlooked in an arbitrary census. PMID:18463068

  10. Serological response of chickens to Salmonella thompson and Salmonella pullorum infections.

    PubMed

    Williams, J E; Whittemore, A D

    1979-01-01

    Chickens were experimentally infected with Salmonella thompson (serogroup C, paratyphoid) and Salmonella pullorum (serogroup D). Five serological methods and one cultural method were used in detecting the infections. The microantiglobulin test was superior to all other methods for detection of paratyphoid (S. THOMPSON) infection and was followed in efficacy by the microagglutination test, rapid serum plate test, cloacal swab culture, macroscopic tube agglutination test, and rapid whole-blood test, in that order. Birds infected with S. pullorum showed much higher agglutinin titers than the birds infected with paratyphoid. The microagglutination and microantiglobulin tests were not significantly different for detection of pullorum infection and were followed in efficacy by the rapid serum plate, macroscopic tube agglutination, rapid whole-blood, and cloacal swab culture tests, in that order. The cloacal swab culture test was totally inadequate for the detection of pullorum infection. PMID:429534

  11. Backwater at bridges and densely wooded flood plains, Thompson Creek near Clara, Mississippi

    USGS Publications Warehouse

    Colson, B.E.; Ming, C.O.; Arcement, George J.

    1979-01-01

    Floodflow data that will provide a base for evaluating digital models relating to open-channel flow were obtained at 22 sites on streams in Alabama, Louisiana, and Mississippi. Thirty-five floods were measured. Analysis of the data indicated methods currently in use would be inaccurate where densely vegetated flood plains are crossed by highway embankments and single-opening bridges. This atlas presents flood information at the site on Thompson Creek near Clara, MS: Water depths, velocities, and discharges through bridge openings on Thompson Creek near Clara, MS, for flood of March 3, 1971, are shown, together with peak water-surface elevations along embankments and along cross sections. Manning 's roughness coefficient values in different parts of the flood plain are shown on maps, and flood-frequency relations are shown on a graph. (USGS).

  12. Best management practices plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    SciTech Connect

    1996-04-01

    This plan was prepared in support of the Phase II Remedial Design Report (DOE/OR/01-1449&D1) and in accordance with requirements under CERCLA to present the plan for best management practices to be followed during the remediation. This document provides the Environmental Restoration Program with information about spill prevention and control, water quality monitoring, good housekeeping practices, sediment and erosion control measures, and inspections and environmental compliance practices to be used during Phase II of the remediation project for the Lower East Fork Poplar Creek Operable Unit.

  13. Oxbow Conservation Area; Middle Fork John Day River, Annual Report 2001-2002.

    SciTech Connect

    Robertson, Shaun; Smith, Brent; Cochran, Brian

    2003-04-01

    In early 2001, the Confederated Tribes of Warm Springs, through their John Day Basin Office, concluded the acquisition of the Middle Fork Oxbow Ranch. Under a memorandum of agreement with the Bonneville Power Administration (BPA), the Tribes are required to provided BPA an 'annual written report generally describing the real property interests in the Project, HEP analyses undertaken or in progress, and management activities undertaken or in progress'. This report is to be provided to the BPA by 30 April of each year. This is the first annual report filed for the Oxbow Ranch property.

  14. Assessment of the alluvial sediments in the Big Thompson River Valley, Colorado

    USGS Publications Warehouse

    Barnett, Adrienne; Ellefsen, Karl J.

    2000-01-01

    To obtain subsurface geologic information about the alluvium in the Big Thompson River valley, S-wave refraction data were collected along three roads that cross the valley. The refraction data were used to estimate velocities and thickness for a layered-earth model; from these models, three cross sections of the river valley were constructed. These cross sections show the thickness and the gross stratigraphy of the alluvium.

  15. The effect of Thompson and Troian's nonlinear slip condition on Couette flows between concentric rotating cylinders

    NASA Astrophysics Data System (ADS)

    Power, H.; Soavi, J.; Kantachuvesiri, P.; Nieto, C.

    2015-10-01

    In this work, a detailed study of the effect of the Thompson and Troian's nonlinear slip condition on the flow behaviour of a Newtonian incompressible fluid between two concentric rotating cylinders (Couette flow) is considered. In Thompson and Troian's nonlinear condition, the slip length on the Navier slip condition is considered to be a function of the tangential shear rate at the solid surface instead of being a constant. The resulting formulation presents an apparent singularity on the slip length when a critical shear rate is approached. By considering this type of nonlinear slip condition, it is possible to predict complex characteristics of the flow field not previously reported in the literature, and to show the effect of nonlinear slip on the inverted velocity profiles previously observed in the linear slip case. Particular attention is given to the behaviour of the flow field near the critical shear rate. In such a limit, it is found that the flow field tends to slip flow with a finite slip length. Consequently, previous critique on the singular behaviour of Thompson and Troian's nonlinear model is not valid in the present case.

  16. Optimizing Weather and Research Forecast (WRF) Thompson cloud microphysics on Intel Many Integrated Core (MIC)

    NASA Astrophysics Data System (ADS)

    Mielikainen, Jarno; Huang, Bormin; Huang, Allen

    2014-05-01

    The Thompson cloud microphysics scheme is a sophisticated cloud microphysics scheme in the Weather Research and Forecasting (WRF) model. The scheme is very suitable for massively parallel computation as there are no interactions among horizontal grid points. Compared to the earlier microphysics schemes, the Thompson scheme incorporates a large number of improvements. Thus, we have optimized the speed of this important part of WRF. Intel Many Integrated Core (MIC) ushers in a new era of supercomputing speed, performance, and compatibility. It allows the developers to run code at trillions of calculations per second using the familiar programming model. In this paper, we present our results of optimizing the Thompson microphysics scheme on Intel Many Integrated Core Architecture (MIC) hardware. The Intel Xeon Phi coprocessor is the first product based on Intel MIC architecture, and it consists of up to 61 cores connected by a high performance on-die bidirectional interconnect. The coprocessor supports all important Intel development tools. Thus, the development environment is familiar one to a vast number of CPU developers. Although, getting a maximum performance out of MICs will require using some novel optimization techniques. Those optimization techniques are discusses in this paper. The results show that the optimization improved MIC performance by 3.4x. Furthermore, the optimized MIC code is 7.0x faster than the optimized multi-threaded code on the four CPU cores of a single socket Intel Xeon E5-2603 running at 1.8 GHz.

  17. Thirty Years Later: Reflections of the Big Thompson Flood, Colorado, 1976 to 2006

    NASA Astrophysics Data System (ADS)

    Jarrett, R. D.; Costa, J. E.; Brunstein, F. C.; Quesenberry, C. A.; Vandas, S. J.; Capesius, J. P.; O'Neill, G. B.

    2006-12-01

    Thirty years ago, over 300 mm of rain fell in about 4 to 6 hours in the middle reaches of the Big Thompson River Basin during the devastating flash flood on July 31, 1976. The rainstorm produced flood discharges that exceeded 40 m3/s/km2. A peak discharge of 883 m3/s was estimated at the Big Thompson River near Drake streamflow-gaging station. The raging waters left 144 people dead, 250 injured, and over 800 people were evacuated by helicopter. Four-hundred eighteen homes and businesses were destroyed, as well as 438 automobiles, and damage to infrastructure left the canyon reachable only via helicopter. Total damage was estimated in excess of $116 million (2006 dollars). Natural hazards similar to the Big Thompson flood are rare, but the probability of a similar event hitting the Front Range, other parts of Colorado, or other parts of the Nation is real. Although much smaller in scale than the Big Thompson flood, several flash floods have happened during the monsoon in early July 2006 in the Colorado foothills that reemphasized the hazards associated with flash flooding. The U.S. Geological Survey (USGS) conducts flood research to help understand and predict the magnitude and likelihood of large streamflow events such as the Big Thompson flood. A summary of hydrologic conditions of the 1976 flood, what the 1976 flood can teach us about flash floods, a description of some of the advances in USGS flood science as a consequence of this disaster, and lessons that we learned to help reduce loss of life from this extraordinary flash flood are discussed. In the 30 years since the Big Thompson flood, there have been important advances in streamflow monitoring and flood warning. The National Weather Service (NWS) NEXRAD radar allows real-time monitoring of precipitation in most places in the United States. The USGS currently (2006) operates about 7,250 real-time streamflow-gaging stations in the United States that are monitored by the USGS, the NWS, and emergency managers

  18. The Replication Checkpoint Protects Fork Stability by Releasing Transcribed Genes from Nuclear Pores

    PubMed Central

    Bermejo, Rodrigo; Capra, Thelma; Jossen, Rachel; Colosio, Arianna; Frattini, Camilla; Carotenuto, Walter; Cocito, Andrea; Doksani, Ylli; Klein, Hannah; Gómez-González, Belén; Aguilera, Andrés; Katou, Yuki; Shirahige, Katsuhiko; Foiani, Marco

    2011-01-01

    Summary Transcription hinders replication fork progression and stability, and the Mec1/ATR checkpoint protects fork integrity. Examining checkpoint-dependent mechanisms controlling fork stability, we find that fork reversal and dormant origin firing due to checkpoint defects are rescued in checkpoint mutants lacking THO, TREX-2, or inner-basket nucleoporins. Gene gating tethers transcribed genes to the nuclear periphery and is counteracted by checkpoint kinases through phosphorylation of nucleoporins such as Mlp1. Checkpoint mutants fail to detach transcribed genes from nuclear pores, thus generating topological impediments for incoming forks. Releasing this topological complexity by introducing a double-strand break between a fork and a transcribed unit prevents fork collapse. Mlp1 mutants mimicking constitutive checkpoint-dependent phosphorylation also alleviate checkpoint defects. We propose that the checkpoint assists fork progression and stability at transcribed genes by phosphorylating key nucleoporins and counteracting gene gating, thus neutralizing the topological tension generated at nuclear pore gated genes. PMID:21784245

  19. A COMPREHENISVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    EPA Science Inventory

    There is an urgent need for EPA to develop protocols for establishing Total Maximum Daily Loads (TMDLs) in streams, lakes and estuaries. A cooperative TMDL field data collection project between ORD and Region 4 is ongoing in the South Fork Broad River Watershed (SFBR), a 245.18 ...

  20. A Multi-Fork Z-Axis Quartz Micromachined Gyroscope

    PubMed Central

    Feng, Lihui; Zhao, Ke; Sun, Yunan; Cui, Jianmin; Cui, Fang; Yang, Aiying

    2013-01-01

    A novel multi-fork z-axis gyroscope is presented in this paper. Different from traditional quartz gyroscopes, the lateral electrodes of the sense beam can be arranged in simple patterns; as a result, the fabrication is simplified. High sensitivity is achieved by the multi-fork design. The working principles are introduced, while the finite element method (FEM) is used to simulate the modal and sensitivity. A quartz fork is fabricated, and a prototype is assembled. Impedance testing shows that the drive frequency and sense frequency are similar to the simulations, and the quality factor is approximately 10,000 in air. The scale factor is measured to be 18.134 mV/(°/s) and the nonlinearity is 0.40% in a full-scale input range of ±250 °/s. PMID:24048339

  1. Acoustic Doppler current profiling from the JGOFS Arabian sea cruises aboard the RV T.G. Thompson

    SciTech Connect

    Kim, Hyun-Sook; Flagg, C.N.; Shi, Yan

    1996-06-01

    Acoustic Doppler current profiler (ADCP) data is part of the core data for the U.S. JGOFS Arabian Sea project, along with hydrographic and nutrient data. Seventeen cruises are scheduled to take place between September 1994 and January 1996 on the R/V T.G. Thompson. They are numbered consecutively from the ship`s commissioning with the first JGOFS cruise designated TN039. Table 1 lists start and end dates of each cruise with its mission. All but the first cruise have been or will be staged from Muscat, Oman. Each cruise is scheduled for a duration of between two weeks and one month. Seven of the cruises, referred to as process cruises, follow a standard cruise track, taking hydrographic, chemical and biological measurements. The rest of the cruises, which take place generally within the standard cruise region defined by a set track, are for the deployment and recovery of moored equipments and towing of a SeaSoar. ADCP data are collected using an autonomous data acquisition system developed for ship-of-opportunity cruises, named the AutoADCP system. The system is an extension of RD instrument`s DAS version 2.48 using enhancements made possible with {open_quotes}user-exit{close_quotes} programs. It makes it possible to collect ADCP data without the constant monitoring usually necessary and insures constant data coverage and uniform data quality.

  2. Bank stability and channel width adjustment, East Fork River, Wyoming.

    USGS Publications Warehouse

    Andrews, E.D.

    1982-01-01

    Frequent surveys of eight cross sections located in self-formed reaches of the East Fork River, Wyoming, during the 1974 snowmelt flood showed a close relation between channel morphology and scour and fill. Those cross sections narrower than the mean reach width filled at discharges less than bankfull and scoured at discharges greater than bankfull. Those cross sections wider than the mean reach width scoured at discharges less than bankfull and filled at discharges greater than bankfull. Bank stability, and to some extent the adjustment of stream channel width, in the East Fork River study reach appears to be controlled by the processes of scour and fill. -from Author

  3. Replication Termination: Containing Fork Fusion-Mediated Pathologies in Escherichia coli.

    PubMed

    Dimude, Juachi U; Midgley-Smith, Sarah L; Stein, Monja; Rudolph, Christian J

    2016-01-01

    Duplication of bacterial chromosomes is initiated via the assembly of two replication forks at a single defined origin. Forks proceed bi-directionally until they fuse in a specialised termination area opposite the origin. This area is flanked by polar replication fork pause sites that allow forks to enter but not to leave. The precise function of this replication fork trap has remained enigmatic, as no obvious phenotypes have been associated with its inactivation. However, the fork trap becomes a serious problem to cells if the second fork is stalled at an impediment, as replication cannot be completed, suggesting that a significant evolutionary advantage for maintaining this chromosomal arrangement must exist. Recently, we demonstrated that head-on fusion of replication forks can trigger over-replication of the chromosome. This over-replication is normally prevented by a number of proteins including RecG helicase and 3' exonucleases. However, even in the absence of these proteins it can be safely contained within the replication fork trap, highlighting that multiple systems might be involved in coordinating replication fork fusions. Here, we discuss whether considering the problems associated with head-on replication fork fusion events helps us to better understand the important role of the replication fork trap in cellular metabolism. PMID:27463728

  4. Functional Analysis of DNA Replication Fork Reversal Catalyzed by Mycobacterium tuberculosis RuvAB Proteins*

    PubMed Central

    Khanduja, Jasbeer Singh; Muniyappa, K.

    2012-01-01

    Initially discovered in Escherichia coli, RuvAB proteins are ubiquitous in bacteria and play a dual role as molecular motor proteins responsible for branch migration of the Holliday junction(s) and reversal of stalled replication forks. Despite mounting genetic evidence for a crucial role of RuvA and RuvB proteins in reversal of stalled replication forks, the mechanistic aspects of this process are still not fully understood. Here, we elucidate the ability of Mycobacterium tuberculosis RuvAB (MtRuvAB) complex to catalyze the reversal of replication forks using a range of DNA replication fork substrates. Our studies show that MtRuvAB, unlike E. coli RuvAB, is able to drive replication fork reversal via the formation of Holliday junction intermediates, suggesting that RuvAB-catalyzed fork reversal involves concerted unwinding and annealing of nascent leading and lagging strands. We also demonstrate the reversal of replication forks carrying hemi-replicated DNA, indicating that MtRuvAB complex-catalyzed fork reversal is independent of symmetry at the fork junction. The fork reversal reaction catalyzed by MtRuvAB is coupled to ATP hydrolysis, is processive, and culminates in the formation of an extended reverse DNA arm. Notably, we found that sequence heterology failed to impede the fork reversal activity of MtRuvAB. We discuss the implications of these results in the context of recognition and processing of varied types of replication fork structures by RuvAB proteins. PMID:22094465

  5. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... contract for Big South Fork National Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public... National Park Service Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY... contract for the conduct of certain visitor services within Big South Fork National Recreation...

  6. Replication Termination: Containing Fork Fusion-Mediated Pathologies in Escherichia coli

    PubMed Central

    Dimude, Juachi U.; Midgley-Smith, Sarah L.; Stein, Monja; Rudolph, Christian J.

    2016-01-01

    Duplication of bacterial chromosomes is initiated via the assembly of two replication forks at a single defined origin. Forks proceed bi-directionally until they fuse in a specialised termination area opposite the origin. This area is flanked by polar replication fork pause sites that allow forks to enter but not to leave. The precise function of this replication fork trap has remained enigmatic, as no obvious phenotypes have been associated with its inactivation. However, the fork trap becomes a serious problem to cells if the second fork is stalled at an impediment, as replication cannot be completed, suggesting that a significant evolutionary advantage for maintaining this chromosomal arrangement must exist. Recently, we demonstrated that head-on fusion of replication forks can trigger over-replication of the chromosome. This over-replication is normally prevented by a number of proteins including RecG helicase and 3’ exonucleases. However, even in the absence of these proteins it can be safely contained within the replication fork trap, highlighting that multiple systems might be involved in coordinating replication fork fusions. Here, we discuss whether considering the problems associated with head-on replication fork fusion events helps us to better understand the important role of the replication fork trap in cellular metabolism. PMID:27463728

  7. Revisiting Intel Xeon Phi optimization of Thompson cloud microphysics scheme in Weather Research and Forecasting (WRF) model

    NASA Astrophysics Data System (ADS)

    Mielikainen, Jarno; Huang, Bormin; Huang, Allen

    2015-10-01

    The Thompson cloud microphysics scheme is a sophisticated cloud microphysics scheme in the Weather Research and Forecasting (WRF) model. The scheme is very suitable for massively parallel computation as there are no interactions among horizontal grid points. Compared to the earlier microphysics schemes, the Thompson scheme incorporates a large number of improvements. Thus, we have optimized the speed of this important part of WRF. Intel Many Integrated Core (MIC) ushers in a new era of supercomputing speed, performance, and compatibility. It allows the developers to run code at trillions of calculations per second using the familiar programming model. In this paper, we present our results of optimizing the Thompson microphysics scheme on Intel Many Integrated Core Architecture (MIC) hardware. The Intel Xeon Phi coprocessor is the first product based on Intel MIC architecture, and it consists of up to 61 cores connected by a high performance on-die bidirectional interconnect. The coprocessor supports all important Intel development tools. Thus, the development environment is familiar one to a vast number of CPU developers. Although, getting a maximum performance out of MICs will require using some novel optimization techniques. New optimizations for an updated Thompson scheme are discusses in this paper. The optimizations improved the performance of the original Thompson code on Xeon Phi 7120P by a factor of 1.8x. Furthermore, the same optimizations improved the performance of the Thompson on a dual socket configuration of eight core Intel Xeon E5-2670 CPUs by a factor of 1.8x compared to the original Thompson code.

  8. Effects of ambient oxidant air pollution in the San Joaquin Valley on Thompson seedless grapes

    SciTech Connect

    Brewer, R.F.; Ashcroft, R.

    1984-01-01

    Mature Thompson seedless grape vines were enclosed in specially constructed plastic covered chambers supplied with carbon filtered and non-filtered (ambient) air from time of bud break through leaf drop. Effects on vegetative growth and fruiting were determined for three seasons. No effects on fruit production were measured the first season after covering but vegetative growth increased 12% in chambers supplied with filtered air. By the third season fruit yields were 27.5% higher in the filtered as compared with ambient chambers. The only visible symptoms associated with exposure to the oxidants was accelerated senescence which appeared 3 weeks to 1 month earlier on vines receiving ambient or nonfiltered air.

  9. Ten Things You Should Do with a Tuning Fork

    ERIC Educational Resources Information Center

    Lincoln, James

    2013-01-01

    Tuning forks are wonderful tools for teaching physics. Every physics classroom should have several and every physics student should be taught how to use them. In this article, I highlight 10 enriching demonstrations that most teachers might not know, as well as provide tips to enhance the demonstrations teachers might already be doing. Some of…

  10. 80. Laurel Fork Creek Bridge #2. Example of a concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    80. Laurel Fork Creek Bridge #2. Example of a concrete slab bridge with T beams. It was built in 1937 and the wing walls were faced with stone to blend with its surroundings. Looking northeast. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  11. 132. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    132. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN FALLS COUNTY, SOUTH OF HANSEN, IDAHO; POWER GATES FOR HYDRO-ELECTRIC. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  12. The Fork in the Road: Histone Partitioning During DNA Replication

    PubMed Central

    Annunziato, Anthony T.

    2015-01-01

    In the following discussion the distribution of histones at the replication fork is examined, with specific attention paid to the question of H3/H4 tetramer "splitting." After a presentation of early experiments surrounding this topic, more recent contributions are detailed. The implications of these findings with respect to the transmission of histone modifications and epigenetic models are also addressed. PMID:26110314

  13. The Sky Is Falling: A Study Of Particulates... . [Project ECOLogy ELE Pak, Thompson Pak].

    ERIC Educational Resources Information Center

    Thompson, Dennis W.

    This is one of a series of units for environmental education developed by the Highline Public Schools. Designed for secondary school science classes, the unit is concerned with particulate matter of air pollution. Five lessons are included. The lessons include construction of equipment and collecting data. (RH)

  14. Air Pollution: What You Can & Can't See. [Project ECOLogy ELE Pak, Thompson Pak.].

    ERIC Educational Resources Information Center

    Thompson, Dennis W.

    This is one of a series of units for environmental education developed by the Highline Public Schools. The unit on air pollution is designed for secondary school students in grades 7 through 12. There are five lessons in the unit; since some of the activities can take as long as 90 days, use of the materials needs to be carefully planned. Each…

  15. South Fork Salmon River Watershed Restoration, 2008-2009 Annual Report.

    SciTech Connect

    Reaney, Mark D.

    2009-04-15

    The watershed restoration work elements within the project area, the South Fork Salmon River Watershed, follow the watershed restoration approach adopted by the Nez Perce Tribe Department of Fisheries Resource Management (DFRM) - Watershed Division. The vision of the Nez Perce Tribe DFRM-Watershed Division focuses on protecting, restoring, and enhancing watersheds and treaty resources within the ceded territory of the Nez Perce Tribe under the Treaty of 1855 with the United States Federal Government. The program uses a holistic approach, which encompasses entire watersheds, ridge top to ridge top, emphasizing all cultural aspects and strategies that rely on natural fish production and healthy river ecosystems. The Nez Perce Tribe DFRM-Watershed Division strives towards maximizing historic ecosystem productivity and health for the restoration of anadromous and resident fish populations and the habitat on which all depend on for future generations Originally, this project was funded to create a step/pool stream channel that was appropriate to restore fish passage where the 'Glory Hole Cascade' is currently located at the Stibnite Mine. Due to unforeseen circumstances at the time, the project is unable to move forward as planned and a request for a change in scope of the project and an expansion of the geographic area in which to complete project work was submitted. No additional funds were being requested. The ultimate goal of this project is to work with the holistic, ridge top to ridge top approach to protect and restore the ecological and biological functions of the South Fork Salmon River Watershed to assist in the recovery of threatened and endangered anadromous and resident fish species. FY 2008 Work Elements included two aquatic organism passage (AOP) projects to restore habitat connectivity to two fish-bearing tributaries to the East Fork South Fork Salmon River, Salt and Profile Creeks. The Work Elements also included road survey and assessment activities

  16. Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability

    PubMed Central

    Schalbetter, Stephanie A.; Mansoubi, Sahar; Chambers, Anna L.; Downs, Jessica A.; Baxter, Jonathan

    2015-01-01

    Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA precatenation formed. Although fork rotation predominates at replication termination, in vitro studies have suggested that it also occurs frequently during elongation. However, the factors that influence fork rotation and how rotation and precatenation may influence other replication-associated processes are unknown. Here we analyze the causes and consequences of fork rotation in budding yeast. We find that fork rotation and precatenation preferentially occur in contexts that inhibit topoisomerase action ahead of the fork, including stable protein–DNA fragile sites and termination. However, generally, fork rotation and precatenation are actively inhibited by Timeless/Tof1 and Tipin/Csm3. In the absence of Tof1/Timeless, excessive fork rotation and precatenation cause extensive DNA damage following DNA replication. With Tof1, damage related to precatenation is focused on the fragile protein–DNA sites where fork rotation is induced. We conclude that although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin. PMID:26240319

  17. Termination of DNA replication forks: “Breaking up is hard to do”

    PubMed Central

    Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

    2015-01-01

    To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component – Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field. PMID:25835602

  18. Mitomycin C reduces abundance of replication forks but not rates of fork progression in primary and transformed human cells

    PubMed Central

    Kehrli, Keffy; Sidorova, Julia M.

    2014-01-01

    DNA crosslinks can block replication in vitro and slow down S phase progression in vivo. We characterized the effect of mitomycin C crosslinker on S phase globally and on individual replication forks in wild type and FANCD2-deficient human cells. FANCD2 is critical to crosslink repair, and is also implicated in facilitating DNA replication. We used DNA fiber analysis to demonstrate persistent reduction in abundance but not progression rate of replication forks during an S phase of MMC-treated cells. FANCD2 deficiency did not eliminate this phenotype. Immunoprecipitation of EdU-labeled DNA indicated that replication was not suppressed in the domains that were undergoing response to MMC as marked by the presence of γH2AX, and in fact γH2AX was overrepresented on DNA that had replicated immediately after MMC in wild type through less so in FANCD2-depleted cells. FANCD2-depleted cells also produced fewer tracks of uninterrupted replication of up to 240Kb long, regardless of MMC treatment. Overall, the data suggest that crosslinks may not pose a block to S phase as a whole, but instead profoundly change its progress by reducing density of replication forks and causing at least a fraction of forks to operate within a DNA damage response-altered chromatin. PMID:25580447

  19. IAC Energy Assessment of Spanish Fork Plant

    SciTech Connect

    2001-08-01

    This case study is the latest in a series on industrial firms who are implementing energy efficient technologies and system improvements into their manufacturing processes. The case studies document the activities, savings, and lessons learned on these projects.

  20. Evaluation of the Dallas Thompson Riverscreen Site on the Touchet River.

    SciTech Connect

    Chamness, Mickie

    2007-07-25

    Riverscreen irrigation pumps are a relatively new design in which the pump intake floats on the river surface, pulling water in only from the bottom side and surrounded by a self-cleaning screen. The Walla Walla County Conservation District recently started replacing old pump screens with the Riverscreen and was interested in whether the screens are protective of juvenile salmonids. Pacific Northwest National Laboratory evaluated approach velocities and operations at the Riverscreen installation on the Dallas Thompson property, approximately 3 mi. north of Touchet, Washington and 300 ft north of Hofer Dam, on June 18, 2007. Evaluation of this site consisted of underwater videography and water velocity measurements. The Dallas Thompson Riverscreen was pumping approximately 930 gpm during our evaluation, which is close to the maximum pumping rate for this model. Underwater videography showed only slow movement of water-borne particulates toward the pump intake, and the screen material was clean. All water velocity measurements were taken below the pump intake opening and between 3 to 6 in. from the screen face. All approach velocities (flow toward the screen and pump) were below National Marine Fisheries Service draft guidelines for juvenile fish screens.

  1. Test pilots 1962 - Thompson, McKay, Dana, Armstrong, Peterson, Butchart, Walker

    NASA Technical Reports Server (NTRS)

    1962-01-01

    A group photo of NASA research pilots at the front door of the Flight Research Center headquarters building. In the front row are (left to right) Milt Thompson, Jack McKay, and Bill Dana. All three flew the X-15, and Thompson and Dana were also involved in the lifting body flights. McKay was injured in a crash landing in X-15 #2. Although he recovered, the injuries eventually forced him to retire from research flying. In the back row (left to right) are Neil Armstrong, Bruce Peterson, Stanley Butchart, and Joe Walker. Armstrong and Walker also both flew the X-15. Soon after this photo was taken, Armstrong was selected as an astronaut, and seven years later became the first man to walk on the Moon. Walker made the highest flight in the X-15, reaching 354,200 feet. He then went on to fly the Lunar Landing Research Vehicle, and was killed on June 8, 1966 when his F-104N collided with the XB-70. Peterson made the first flight in the HL-10 lifting body, and was later badly injured in the crash of the M2-F2 lifting body. Butchart flew a wide range of research missions in the 1950s, and was the B-29 drop plane pilot for a number of rocket flight.

  2. Effect of heat treatment, pH, sugar concentration, and metal addition on green color retention in homogenized puree of Thompson seedless grape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Homogenized puree of Thompson seedless (Vitis vinifera ‘Thompson Seedless’) grape was treated under different conditions, including heating time (5-30 min), temperature (20-80°C) and pH (2-10). Treatments with separate additions of glucose, fructose, and sucrose at concentrations of 100-600 g/L and ...

  3. Schools of Fort Gay-Thompson, West Virginia, Spring 1973. A Report to the Fort Gay-Thompson School Community Council by the Bureau of School Service, University of Kentucky.

    ERIC Educational Resources Information Center

    Banks, Carl; And Others

    The initial step in the evaluation of the Fort Gay-Thompson School Community Council's program was to establish baseline data prerequisite to measuring the change in the program's influence. Surveys were made of the typical program phases regarded as germane to evaluation. Thus, data were obtained on: pupils--enrollment trends, achievement levels,…

  4. Analysis of fork-join program response times on multiprocessors

    SciTech Connect

    Towsley, D.; Stankovic, J.A. . Dept. of Computer and Information Science); Rommel, C.G. )

    1990-07-01

    In this paper, the authors develop analytic models for a shared memory multiprocessor that executes fork-join parallel programs. Here a fork-join program is one that consists of a set of n {ge} 1 parallel tasks. All of the tasks of a program arrive simultaneously to the system and the job is assumed to complete when the last task completes. They develop and analyze models for two processor sharing policies, called task scheduling processor sharing and job scheduling processor sharing. The first policy schedules tasks independently of each other and allows parallel execution of an individual program, whereas the second policy schedules each job as a unit and thereby does not allow parallel execution of an individual program.

  5. Optimal information provision for maximizing flow in a forked lattice

    NASA Astrophysics Data System (ADS)

    Imai, Takeaki; Nishinari, Katsuhiro

    2015-06-01

    In a forked road, the provision of inappropriate information to car drivers sometimes leads to undesirable situations such as one-sided congestion, which is called the hunting phenomenon in real traffic. To address such problems, we propose a forked exclusion model and investigate the behavior of traffic flow in two routes, providing various types of information to a limited number of traveling particles according to the share rate of information. To analytically understand the phenomena, we develop a coarse-grained representation of the model. By analyzing the model, we find the most effective types of information to minimize particles' travel time and the existence of an optimal share rate according to route conditions.

  6. Ten Things You Should Do with a Tuning Fork

    NASA Astrophysics Data System (ADS)

    Lincoln, James

    2013-03-01

    Tuning forks are wonderful tools for teaching physics. Every physics classroom should have several and every physics student should be taught how to use them. In this article, I highlight 10 enriching demonstrations that most teachers might not know, as well as provide tips to enhance the demonstrations teachers might already be doing. Some of these demonstrations have appeared in literature before, but this paper will serve as a collection of ideas that will be helpful for both the beginner and master teacher.

  7. NORTH FORK OF THE AMERICAN RIVER WILDERNESS STUDY AREA, CALIFORNIA.

    USGS Publications Warehouse

    Harwood, David S.; Federspiel, Francis E.

    1984-01-01

    Mineral-resource surveys of the North Fork of the American River Wilderness study area, California have identified a zone of substantiated resource potential for gold and silver. Zones of probable gold and silver potential occur in the eastern part of the area between the Wubbena and La Trinidad mines and locally around the Marrs mine. A zone with probable chromium potential occurs in the serpentinite belt along the western border of the area. No energy resources were identified in this study.

  8. Stalled replication forks within heterochromatin require ATRX for protection.

    PubMed

    Huh, M S; Ivanochko, D; Hashem, L E; Curtin, M; Delorme, M; Goodall, E; Yan, K; Picketts, D J

    2016-01-01

    Expansive growth of neural progenitor cells (NPCs) is a prerequisite to the temporal waves of neuronal differentiation that generate the six-layered neocortex, while also placing a heavy burden on proteins that regulate chromatin packaging and genome integrity. This problem is further reflected by the growing number of developmental disorders caused by mutations in chromatin regulators. ATRX gene mutations cause a severe intellectual disability disorder (α-thalassemia mental retardation X-linked (ATRX) syndrome; OMIM no. 301040), characterized by microcephaly, urogenital abnormalities and α-thalassemia. Although the ATRX protein is required for the maintenance of repetitive DNA within heterochromatin, how this translates to disease pathogenesis remain poorly understood and was a focus of this study. We demonstrate that Atrx(FoxG1Cre) forebrain-specific conditional knockout mice display poly(ADP-ribose) polymerase-1 (Parp-1) hyperactivation during neurogenesis and generate fewer late-born Cux1- and Brn2-positive neurons that accounts for the reduced cortical size. Moreover, DNA damage, induced Parp-1 and Atm activation is elevated in progenitor cells and contributes to their increased level of cell death. ATRX-null HeLa cells are similarly sensitive to hydroxyurea-induced replication stress, accumulate DNA damage and proliferate poorly. Impaired BRCA1-RAD51 colocalization and PARP-1 hyperactivation indicated that stalled replication forks are not efficiently protected. DNA fiber assays confirmed that MRE11 degradation of stalled replication forks was rampant in the absence of ATRX or DAXX. Indeed, fork degradation in ATRX-null cells could be attenuated by treatment with the MRE11 inhibitor mirin, or exacerbated by inhibiting PARP-1 activity. Taken together, these results suggest that ATRX is required to limit replication stress during cellular proliferation, whereas upregulation of PARP-1 activity functions as a compensatory mechanism to protect stalled forks

  9. Stalled replication forks within heterochromatin require ATRX for protection

    PubMed Central

    Huh, M S; Ivanochko, D; Hashem, L E; Curtin, M; Delorme, M; Goodall, E; Yan, K; Picketts, D J

    2016-01-01

    Expansive growth of neural progenitor cells (NPCs) is a prerequisite to the temporal waves of neuronal differentiation that generate the six-layered neocortex, while also placing a heavy burden on proteins that regulate chromatin packaging and genome integrity. This problem is further reflected by the growing number of developmental disorders caused by mutations in chromatin regulators. ATRX gene mutations cause a severe intellectual disability disorder (α-thalassemia mental retardation X-linked (ATRX) syndrome; OMIM no. 301040), characterized by microcephaly, urogenital abnormalities and α-thalassemia. Although the ATRX protein is required for the maintenance of repetitive DNA within heterochromatin, how this translates to disease pathogenesis remain poorly understood and was a focus of this study. We demonstrate that AtrxFoxG1Cre forebrain-specific conditional knockout mice display poly(ADP-ribose) polymerase-1 (Parp-1) hyperactivation during neurogenesis and generate fewer late-born Cux1- and Brn2-positive neurons that accounts for the reduced cortical size. Moreover, DNA damage, induced Parp-1 and Atm activation is elevated in progenitor cells and contributes to their increased level of cell death. ATRX-null HeLa cells are similarly sensitive to hydroxyurea-induced replication stress, accumulate DNA damage and proliferate poorly. Impaired BRCA1-RAD51 colocalization and PARP-1 hyperactivation indicated that stalled replication forks are not efficiently protected. DNA fiber assays confirmed that MRE11 degradation of stalled replication forks was rampant in the absence of ATRX or DAXX. Indeed, fork degradation in ATRX-null cells could be attenuated by treatment with the MRE11 inhibitor mirin, or exacerbated by inhibiting PARP-1 activity. Taken together, these results suggest that ATRX is required to limit replication stress during cellular proliferation, whereas upregulation of PARP-1 activity functions as a compensatory mechanism to protect stalled forks

  10. Mitochondrial transcription termination factor 1 directs polar replication fork pausing

    PubMed Central

    Shi, Yonghong; Posse, Viktor; Zhu, Xuefeng; Hyvärinen, Anne K.; Jacobs, Howard T.; Falkenberg, Maria; Gustafsson, Claes M.

    2016-01-01

    During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can cause replication fork collapse and genomic instability. To avoid this problem, a replication fork barrier protein is situated downstream of rDNA, there preventing replication in the direction opposite rDNA transcription. A potential candidate for a similar function in mitochondria is the mitochondrial transcription termination factor 1 (MTERF1, also denoted mTERF), which binds to a sequence just downstream of the ribosomal transcription unit. Previous studies have shown that MTERF1 prevents antisense transcription over the ribosomal RNA genes, a process which we here show to be independent of the transcription elongation factor TEFM. Importantly, we now demonstrate that MTERF1 arrests mitochondrial DNA (mtDNA) replication with distinct polarity. The effect is explained by the ability of MTERF1 to act as a directional contrahelicase, blocking mtDNA unwinding by the mitochondrial helicase TWINKLE. This conclusion is also supported by in vivo evidence that MTERF1 stimulates TWINKLE pausing. We conclude that MTERF1 can direct polar replication fork arrest in mammalian mitochondria. PMID:27112570

  11. Replication fork stability confers chemoresistance in BRCA-deficient cells.

    PubMed

    Ray Chaudhuri, Arnab; Callen, Elsa; Ding, Xia; Gogola, Ewa; Duarte, Alexandra A; Lee, Ji-Eun; Wong, Nancy; Lafarga, Vanessa; Calvo, Jennifer A; Panzarino, Nicholas J; John, Sam; Day, Amanda; Crespo, Anna Vidal; Shen, Binghui; Starnes, Linda M; de Ruiter, Julian R; Daniel, Jeremy A; Konstantinopoulos, Panagiotis A; Cortez, David; Cantor, Sharon B; Fernandez-Capetillo, Oscar; Ge, Kai; Jonkers, Jos; Rottenberg, Sven; Sharan, Shyam K; Nussenzweig, André

    2016-07-21

    Cells deficient in the Brca1 and Brca2 genes have reduced capacity to repair DNA double-strand breaks by homologous recombination and consequently are hypersensitive to DNA-damaging agents, including cisplatin and poly(ADP-ribose) polymerase (PARP) inhibitors. Here we show that loss of the MLL3/4 complex protein, PTIP, protects Brca1/2-deficient cells from DNA damage and rescues the lethality of Brca2-deficient embryonic stem cells. However, PTIP deficiency does not restore homologous recombination activity at double-strand breaks. Instead, its absence inhibits the recruitment of the MRE11 nuclease to stalled replication forks, which in turn protects nascent DNA strands from extensive degradation. More generally, acquisition of PARP inhibitors and cisplatin resistance is associated with replication fork protection in Brca2-deficient tumour cells that do not develop Brca2 reversion mutations. Disruption of multiple proteins, including PARP1 and CHD4, leads to the same end point of replication fork protection, highlighting the complexities by which tumour cells evade chemotherapeutic interventions and acquire drug resistance. PMID:27443740

  12. 78 FR 60698 - Safety Zone, Lucas Oil Drag Boat Racing Series; Thompson Bay, Lake Havasu City, AZ.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone, Lucas Oil Drag Boat Racing Series; Thompson..., AZ for the Lucas Oil Drag Boat Racing Series. This temporary safety zone is necessary to...

  13. Moore-Gibson-Thompson equation with memory, part I: exponential decay of energy

    NASA Astrophysics Data System (ADS)

    Lasiecka, Irena; Wang, Xiaojun

    2016-04-01

    We are interested in the Moore-Gibson-Thompson equation with memory τ{u}_{ttt}+ α u_{tt}+c2Au+bAu_t -int_0tg(t-s)A w(s){d}s=0. This model arises in high-frequency ultrasound applications accounting for thermal flux and molecular relaxation times. According to revisited extended irreversible thermodynamics, thermal flux relaxation leads to the third-order derivative in time while molecular relaxation leads to non-local effects governed by memory terms. The resulting model is of hyperbolic type with viscous effects. We first classify the memory into three types. Then, we study how a memory term creates damping mechanism and how the memory causes energy decay even in the cases when the original memoryless system is unstable.

  14. Thompson and Hamilton type IV Freiberg's disease with involvement of multiple epiphyses of both feet.

    PubMed

    Lui, Tun Hing

    2015-01-01

    A 17-year-old boy reported left second and third toe pain after axial loading injury to his left foot. Radiographs showed collapse of the second metatarsal heads and epiphysial irregularities of the fifth metatarsal heads and the condyle of the proximal phalanx of the hallux of both feet. The patient was diagnosed to have Thompson and Hamilton type IV Freiberg's disease. He was screened for epiphysial dysplasia of the other sites. He had on and off bilateral hip and knee pain. Radiographs showed bilateral symmetrical epiphysial abnormalities with morphological change as focal concavity in bilateral femoral heads and fragmentation of the patellar articular surface with preservation of the patellofemoral joint space. PMID:25721826

  15. Recovery of arrested replication forks by homologous recombination is error-prone.

    PubMed

    Iraqui, Ismail; Chekkal, Yasmina; Jmari, Nada; Pietrobon, Violena; Fréon, Karine; Costes, Audrey; Lambert, Sarah A E

    2012-01-01

    Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability. Here, we report that recovery of impeded replication forks by homologous recombination is error-prone. Using a fork-arrest-based assay in fission yeast, we demonstrate that a single collapsed fork can cause mutations and large-scale genomic changes, including deletions and translocations. Fork-arrest-induced gross chromosomal rearrangements are mediated by inappropriate ectopic recombination events at the site of collapsed forks. Inverted repeats near the site of fork collapse stimulate large-scale genomic changes up to 1,500 times over spontaneous events. We also show that the high accuracy of DNA replication during S-phase is impaired by impediments to fork progression, since fork-arrest-induced mutation is due to erroneous DNA synthesis during recovery of replication forks. The mutations caused are small insertions/duplications between short tandem repeats (micro-homology) indicative of replication slippage. Our data establish that collapsed forks, but not stalled forks, recovered by homologous recombination are prone to replication slippage. The inaccuracy of DNA synthesis does not rely on PCNA ubiquitination or trans-lesion-synthesis DNA polymerases, and it is not counteracted by mismatch repair. We propose that deletions/insertions, mediated by micro-homology, leading to copy number variations during replication stress may arise by progression of error-prone replication forks restarted by homologous recombination. PMID:23093942

  16. Regression of Replication Forks Stalled by Leading-strand Template Damage

    PubMed Central

    Gupta, Sankalp; Yeeles, Joseph T. P.; Marians, Kenneth J.

    2014-01-01

    The orderly progression of replication forks formed at the origin of replication in Escherichia coli is challenged by encounters with template damage, slow moving RNA polymerases, and frozen DNA-protein complexes that stall the fork. These stalled forks are foci for genomic instability and must be reactivated. Many models of replication fork reactivation invoke nascent strand regression as an intermediate in the processing of the stalled fork. We have investigated the replication fork regression activity of RecG and RuvAB, two proteins commonly thought to be involved in the process, using a reconstituted DNA replication system where the replisome is stalled by collision with leading-strand template damage. We find that both RecG and RuvAB can regress the stalled fork in the presence of the replisome and SSB; however, RuvAB generates a completely unwound product consisting of the paired nascent leading and lagging strands, whereas RuvC cleaves the Holliday junction generated by RecG-catalyzed fork regression. We also find that RecG stimulates RuvAB-catalyzed regression, presumably because it is more efficient at generating the initial Holliday junction from the stalled fork. PMID:25138216

  17. Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells

    PubMed Central

    Zellweger, Ralph; Dalcher, Damian; Mutreja, Karun; Berti, Matteo; Schmid, Jonas A.; Herrador, Raquel; Vindigni, Alessandro

    2015-01-01

    Replication fork reversal protects forks from breakage after poisoning of Topoisomerase 1. We here investigated fork progression and chromosomal breakage in human cells in response to a panel of sublethal genotoxic treatments, using other topoisomerase poisons, DNA synthesis inhibitors, interstrand cross-linking inducers, and base-damaging agents. We used electron microscopy to visualize fork architecture under these conditions and analyzed the association of specific molecular features with checkpoint activation. Our data identify replication fork uncoupling and reversal as global responses to genotoxic treatments. Both events are frequent even after mild treatments that do not affect fork integrity, nor activate checkpoints. Fork reversal was found to be dependent on the central homologous recombination factor RAD51, which is consistently present at replication forks independently of their breakage, and to be antagonized by poly (ADP-ribose) polymerase/RECQ1-regulated restart. Our work establishes remodeling of uncoupled forks as a pivotal RAD51-regulated response to genotoxic stress in human cells and as a promising target to potentiate cancer chemotherapy. PMID:25733714

  18. Mammalian RAD51 paralogs protect nascent DNA at stalled forks and mediate replication restart

    PubMed Central

    Somyajit, Kumar; Saxena, Sneha; Babu, Sharath; Mishra, Anup; Nagaraju, Ganesh

    2015-01-01

    Mammalian RAD51 paralogs are implicated in the repair of collapsed replication forks by homologous recombination. However, their physiological roles in replication fork maintenance prior to fork collapse remain obscure. Here, we report on the role of RAD51 paralogs in short-term replicative stress devoid of DSBs. We show that RAD51 paralogs localize to nascent DNA and common fragile sites upon replication fork stalling. Strikingly, RAD51 paralogs deficient cells exhibit elevated levels of 53BP1 nuclear bodies and increased DSB formation, the latter being attributed to extensive degradation of nascent DNA at stalled forks. RAD51C and XRCC3 promote the restart of stalled replication in an ATP hydrolysis dependent manner by disengaging RAD51 and other RAD51 paralogs from the halted forks. Notably, we find that Fanconi anemia (FA)-like disorder and breast and ovarian cancer patient derived mutations of RAD51C fails to protect replication fork, exhibit under-replicated genomic regions and elevated micro-nucleation. Taken together, RAD51 paralogs prevent degradation of stalled forks and promote the restart of halted replication to avoid replication fork collapse, thereby maintaining genomic integrity and suppressing tumorigenesis. PMID:26354865

  19. THE FORK AND THE KINASE: A DNA REPLICATION TALE FROM A CHK1 PERSPECTIVE

    PubMed Central

    González Besteiro, Marina A.; Gottifredi, Vanesa

    2014-01-01

    Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. The checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged-DNA. Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Such findings unveil a puzzling connection between Chk1 and DNA-lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, the multifaceted and versatile functions of Chk1 at ongoing forks and replication origins determine the extent and quality of the cellular response to replication stress. PMID:25795119

  20. Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells.

    PubMed

    Zellweger, Ralph; Dalcher, Damian; Mutreja, Karun; Berti, Matteo; Schmid, Jonas A; Herrador, Raquel; Vindigni, Alessandro; Lopes, Massimo

    2015-03-01

    Replication fork reversal protects forks from breakage after poisoning of Topoisomerase 1. We here investigated fork progression and chromosomal breakage in human cells in response to a panel of sublethal genotoxic treatments, using other topoisomerase poisons, DNA synthesis inhibitors, interstrand cross-linking inducers, and base-damaging agents. We used electron microscopy to visualize fork architecture under these conditions and analyzed the association of specific molecular features with checkpoint activation. Our data identify replication fork uncoupling and reversal as global responses to genotoxic treatments. Both events are frequent even after mild treatments that do not affect fork integrity, nor activate checkpoints. Fork reversal was found to be dependent on the central homologous recombination factor RAD51, which is consistently present at replication forks independently of their breakage, and to be antagonized by poly (ADP-ribose) polymerase/RECQ1-regulated restart. Our work establishes remodeling of uncoupled forks as a pivotal RAD51-regulated response to genotoxic stress in human cells and as a promising target to potentiate cancer chemotherapy. PMID:25733714

  1. BRCA1 controls homologous recombination at Tus/Ter-stalled mammalian replication forks.

    PubMed

    Willis, Nicholas A; Chandramouly, Gurushankar; Huang, Bin; Kwok, Amy; Follonier, Cindy; Deng, Chuxia; Scully, Ralph

    2014-06-26

    Replication fork stalling can promote genomic instability, predisposing to cancer and other diseases. Stalled replication forks may be processed by sister chromatid recombination (SCR), generating error-free or error-prone homologous recombination (HR) outcomes. In mammalian cells, a long-standing hypothesis proposes that the major hereditary breast/ovarian cancer predisposition gene products, BRCA1 and BRCA2, control HR/SCR at stalled replication forks. Although BRCA1 and BRCA2 affect replication fork processing, direct evidence that BRCA gene products regulate homologous recombination at stalled chromosomal replication forks is lacking, due to a dearth of tools for studying this process. Here we report that the Escherichia coli Tus/Ter complex can be engineered to induce site-specific replication fork stalling and chromosomal HR/SCR in mouse cells. Tus/Ter-induced homologous recombination entails processing of bidirectionally arrested forks. We find that the Brca1 carboxy (C)-terminal tandem BRCT repeat and regions of Brca1 encoded by exon 11-two Brca1 elements implicated in tumour suppression-control Tus/Ter-induced homologous recombination. Inactivation of either Brca1 or Brca2 increases the absolute frequency of 'long-tract' gene conversions at Tus/Ter-stalled forks, an outcome not observed in response to a site-specific endonuclease-mediated chromosomal double-strand break. Therefore, homologous recombination at stalled forks is regulated differently from homologous recombination at double-strand breaks arising independently of a replication fork. We propose that aberrant long-tract homologous recombination at stalled replication forks contributes to genomic instability and breast/ovarian cancer predisposition in BRCA mutant cells. PMID:24776801

  2. Interaction force microscopy based on quartz tuning fork force sensor

    NASA Astrophysics Data System (ADS)

    Qin, Yexian

    The ability to sense small changes in the interaction force between a scanning probe microscope (SPM) tip and a substrate requires cantilevers with a sharp mechanical resonance. A typical commercially available cantilever in air is characterized by a resonance with a Q factor of 100 ˜ 300. The low Q factor can be attributed to imperfections in the cantilever itself as well as damping effects of the surrounding air. To substantially increase the Q factor, novel concepts are required. For this reason, we have performed a systematic study of quartz tuning fork resonators for possible use with SPMs. We find that tuning fork resonators operating in air are characterized by Q factors in the order of 104, thereby greatly improving the SPM's ability to measure small shifts in the interaction force. By carefully attaching commercially available SPM tips to the tuning fork, it is possible to obtain SPM images using non-contact imaging techniques and analyze the tip-sample interactions. The assembly of uniform molecular monolayers on atomically flat substrates for molecular electronics applications has received widespread attention during the past ten years. Scanning probe techniques are often used to assess substrate topography, molecular ordering and electronic properties, yet little is known about the fundamental tip-molecule interaction. To address this issue we have built an Interaction Force Microscope using a quartz tuning fork to probe tip-molecular monolayer interactions using scanning probe microscopy. The high quality factor and stable resonant frequency of a quartz tuning fork allows accurate measurement of small shifts in the resonant frequency as the tip interacts with the substrate. To permit an accurate measure of surface interaction forces, the electrical and piezomechanical properties of a tuning fork have been calibrated using a fiber optical interferometer. In prior work [1], we have studied molecular layers formed from either 4-Trifluoro

  3. BLACK FORK MOUNTAIN ROADLESS AREA, ARAKANSAS AND OKLAHOMA.

    USGS Publications Warehouse

    Miller, Mary H.

    1984-01-01

    Black Fork Mountain Roadless Area covers about 21 sq mi in the Ouachita National Forest in Polk County, Arkansas and LeFlore County, Oklahoma. On the basis of a mineral survey the area has little promise for the occurrence of metallic mineral resources. Stone and sand and gravel suitable for construction purposes occur in the Jackfork Sandstone and the Stanley Shale which also occur outside the roadless area. Although the potential for gas and oil is unknown and no resource potential was identified, some investigators believe that there is a possibility for the occurrence of gas and oil in the roadless area.

  4. Glue-free tuning fork shear-force microscope

    NASA Astrophysics Data System (ADS)

    Mühlschlegel, P.; Toquant, J.; Pohl, D. W.; Hecht, B.

    2006-01-01

    A scanning near-field optical microscope without any glued parts is described. Key elements are the optical fiber probe/tuning fork junction and the piezotube scanner assembly. In both cases, fixation is achieved by means of controlled pressure and elastic deformation. The avoidance of glued connections was found to improve the Q factor of the shear-force sensor as well as to facilitate the replacement of the fiber probe and other parts of the scanner head. We present approach curves and shear-force images that demonstrate the performance and stability of the system.

  5. Replication fork movement and methylation govern SeqA binding to the Escherichia coli chromosome

    PubMed Central

    Waldminghaus, Torsten; Weigel, Christoph; Skarstad, Kirsten

    2012-01-01

    In Escherichia coli, the SeqA protein binds specifically to GATC sequences which are methylated on the A of the old strand but not on the new strand. Such hemimethylated DNA is produced by progression of the replication forks and lasts until Dam methyltransferase methylates the new strand. It is therefore believed that a region of hemimethylated DNA covered by SeqA follows the replication fork. We show that this is, indeed, the case by using global ChIP on Chip analysis of SeqA in cells synchronized regarding DNA replication. To assess hemimethylation, we developed the first genome-wide method for methylation analysis in bacteria. Since loss of the SeqA protein affects growth rate only during rapid growth when cells contain multiple replication forks, a comparison of rapid and slow growth was performed. In cells with six replication forks per chromosome, the two old forks were found to bind surprisingly little SeqA protein. Cell cycle analysis showed that loss of SeqA from the old forks did not occur at initiation of the new forks, but instead occurs at a time point coinciding with the end of SeqA-dependent origin sequestration. The finding suggests simultaneous origin de-sequestration and loss of SeqA from old replication forks. PMID:22373925

  6. 77 FR 39675 - Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... River Mining AGENCY: Forest Service, USDA. ACTION: Correction--Notice of intent to prepare a supplement... changed to the Whitman District Ranger. This 2012 North Fork Burnt River Mining Record of Decision will replace and supercede the 2004 North Fork Burnt River Mining Record of Decision only where necessary...

  7. 76 FR 6114 - Lincoln National Forest, New Mexico, North Fork Eagle Creek Wells Special Use Authorization

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ... North Fork well field (Village of Ruidoso 2006). The Village of Ruidoso drilled four production wells on... National Forest System land; and (2) Minimizing impacts of groundwater drawdown from this well field to... approximately 31 percent of its water supply from the North Fork well field. During drought conditions prior...

  8. Origin & Evolution of the Grand Forks Human Nutrition Research Center, 1970-90

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the early 1960s William E Cornatzer, MD, PhD, suggested the need for increased USDA research concerning human nutrition, and creation of the Grand Forks Human Nutrition Laboratory (Grand Forks Human Nutrition Research Center). He shared ideas with Senator Milton R. Young of North Dakota, who requ...

  9. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  10. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  11. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  12. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  13. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  14. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Altus Dam and Reservoir, North Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE FLOOD CONTROL REGULATIONS § 208.26 Altus Dam and Reservoir, North Fork Red River, Okla. The Bureau...

  15. 33 CFR 208.33 - Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans. 208.33 Section 208.33 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE FLOOD CONTROL REGULATIONS § 208.33 Cheney Dam and Reservoir, North Fork of Ninnescah River,...

  16. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Altus Dam and Reservoir, North Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE FLOOD CONTROL REGULATIONS § 208.26 Altus Dam and Reservoir, North Fork Red River, Okla. The Bureau...

  17. 33 CFR 208.33 - Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans. 208.33 Section 208.33 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE FLOOD CONTROL REGULATIONS § 208.33 Cheney Dam and Reservoir, North Fork of Ninnescah River,...

  18. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for fork and frame assembly. 1512.14 Section 1512.14 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  19. EVALUATING THE EFFECTIVENESS OF NUTRIENT MANAGEMENT IN THE CLARK FORK-PEND OREILLE WATERSHED

    EPA Science Inventory

    (1) Analyze the nutrient concentration and algal standing crop trend in the Clark Fork River portion of the watershed; (2) Provide a web-based data access system for reporting Clark Fork River monitoring information; and (3) Evaluate the effectiveness of current nutrient reductio...

  20. Mutual interactions of oscillating quartz tuning forks in superfluid 4He

    NASA Astrophysics Data System (ADS)

    Sheshin, G.; Gritsenko, I.; Schmoranzer, D.; Skrbek, L.

    2013-10-01

    The quartz tuning fork has recently become a popular experimental tool for investigations of both classical and quantum turbulence in cryogenic helium. Its increased use in low-temperature experiments and a number of puzzling results obtained in the past have led to many questions concerning the interaction of multiple tuning forks or the interaction of tuning forks with other oscillators. We report measurements performed in He II at low temperatures around 360 mK, on the mutual interaction of tuning forks placed in the same volume of fluid, and examine the responsible mechanisms in an effort to discriminate between acoustic coupling and interaction via quantized vortices. To this end, the interaction of two tuning forks is investigated by analyzing their recorded resonance curves, looking for any nonelectrical crosstalk. Further, the force-velocity characteristics of a detector tuning fork are measured for different operating velocities of a generator tuning fork. As a complementary measurement, the intensity of sound waves is recorded using a set of miniature receivers. We confirm the current knowledge on acoustic emission by tuning forks in He II and verify properties of their radiation patterns. We conclude that in our experiment the interaction is almost entirely mediated by sound waves.

  1. 76 FR 62038 - Boundary Establishment for North Fork Crooked National Wild and Scenic River, Ochoco National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ... Forest Service Boundary Establishment for North Fork Crooked National Wild and Scenic River, Ochoco National Forest, Crook County, Oregon AGENCY: Forest Service, USDA. ACTION: Notice of availability. SUMMARY..., Washington Office, is transmitting the final boundary of the North Fork Crooked National Wild and...

  2. First report of anther smut caused by Microbotryum violaceum on forked catchfly in Turkey

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Forked catchfly (Silene dichotoma Ehrh.), family Caryophyllaceae, is a common and native plant in rangelands and pastures in Turkey. It is also an introduced plant that is widely distributed in North America. In May, 2007 about 20 forked catchfly plants on the campus of Ondokuz Mayis University, i...

  3. Evaluation of Thompson-type trend and monthly weather data models for corn yields in Iowa, Illinois, and Indiana

    NASA Technical Reports Server (NTRS)

    French, V. (Principal Investigator)

    1982-01-01

    An evaluation was made of Thompson-Type models which use trend terms (as a surrogate for technology), meteorological variables based on monthly average temperature, and total precipitation to forecast and estimate corn yields in Iowa, Illinois, and Indiana. Pooled and unpooled Thompson-type models were compared. Neither was found to be consistently superior to the other. Yield reliability indicators show that the models are of limited use for large area yield estimation. The models are objective and consistent with scientific knowledge. Timely yield forecasts and estimates can be made during the growing season by using normals or long range weather forecasts. The models are not costly to operate and are easy to use and understand. The model standard errors of prediction do not provide a useful current measure of modeled yield reliability.

  4. Active Control of Repetitive Structural Transitions between Replication Forks and Holliday Junctions by Werner Syndrome Helicase.

    PubMed

    Shin, Soochul; Lee, Jinwoo; Yoo, Sangwoon; Kulikowicz, Tomasz; Bohr, Vilhelm A; Ahn, Byungchan; Hohng, Sungchul

    2016-08-01

    The reactivation of stalled DNA replication via fork regression invokes Holliday junction formation, branch migration, and the recovery of the replication fork after DNA repair or error-free DNA synthesis. The coordination mechanism for these DNA structural transitions by molecular motors, however, remains unclear. Here we perform single-molecule fluorescence experiments with Werner syndrome protein (WRN) and model replication forks. The Holliday junction is readily formed once the lagging arm is unwound, and migrated unidirectionally with 3.2 ± 0.03 bases/s velocity. The recovery of the replication fork was controlled by branch migration reversal of WRN, resulting in repetitive fork regression. The Holliday junction formation, branch migration, and migration direction reversal are all ATP dependent, revealing that WRN uses the energy of ATP hydrolysis to actively coordinate the structural transitions of DNA. PMID:27427477

  5. 76 FR 35009 - Draft Oil and Gas Management Plan/Environmental Impact Statement for Big South Fork National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... National Park Service Draft Oil and Gas Management Plan/Environmental Impact Statement for Big South Fork... Big South Fork National River and Recreation Area and Obed Wild and Scenic River. SUMMARY: Pursuant to.../DEIS) for the proposed Big South Fork National River and Recreation Area (BISO) and Obed Wild...

  6. Comparison of Stream Restoration and Vegetation Restoration on Stream Temperature in the Middle Fork John Day River, Oregon

    NASA Astrophysics Data System (ADS)

    Diabat, M.; Wondzell, S. M.; Haggerty, R.

    2013-12-01

    Stream temperature is an important component of aquatic ecosystems. During the past century, various anthropogenic activities (such as timber harvest, mining, and agriculture) reduced riparian vegetation and channel complexity along many streams around the world. As a result, stream temperature increased and suitable habitat for cool- and cold-water organisms declined. Stream temperatures are expected to increase even more under future climate. The effects of warmer climate and anthropogenic activities are proposed to be mitigated by restoration projects aimed to reduce stream temperatures. Common restoration practices are replanting natural vegetation along stream banks and restoring channel complexity. The Middle Fork John Day River, in northeastern Oregon, USA is an example of such a process. We modeled stream temperature along a 37-km section of the Middle Fork John Day River for current and projected conditions of climate, restored riparian vegetation along 6.6-km, and restored channel meanders along 1.5 km. Preliminary simulations suggest that if current riparian vegetation remains unchanged, an average summertime air warming of 4°C increased the 7-day average daily maximum (7DADM) by about 1.3°C. However, restored riparian vegetation reduced the 7DADM by about 0.7°C relative to the current temperature. Restored channel meanders reduced the 7DADM by less than 0.05°C relative to the current temperature. These preliminary simulations assume no hyporheic exchange and riparian vegetation that is 10 m tall and has 30% canopy density.

  7. Numerical investigation of three-dimensional transonic flows of Bethe-Zel'dovich-Thompson fluids

    NASA Astrophysics Data System (ADS)

    Cinnella, Paola; Corre, Christophe

    2006-11-01

    Bethe-Zel'dovich-Thompson (BZT) fluids are fluids of the retrograde type (i.e. that superheat when expanded), which exhibit a region of negative values of the Fundamental Derivative of Gasdynamics γ. As a consequence, they display, in the transonic and supersonic regime, nonclassical gasdynamic behaviours, such as rarefaction shock waves and mixed shock/fan waves. The peculiar properties of BZT fluids have received increased interest in recent years because of the possibility of enhancing turbine efficiency in Organic Rankine Cycles (ORCs). The present research provides for the first time a detailed investigation of transonic BZT flows past a 3D configuration, representative of an isolated turbine blade with infinite tip leakage, namely, the ONERA M-6 wing. Since BZT phenomena mainly affect the inviscid flow behavior, the analysis is restricted to the Euler equations, completed by the realistic Martin-Hou equation of state. The governing equations are solved numerically using a structured flow-solver based on a third-order accurate centred scheme. The results are validated through systematic comparisons with an unstructured multidimensional upwind solver. An investigation of the flow patterns for several choices of the upstream thermodynamic conditions is provided, showing the complexity of the 3D aerodynamics of BZT fluids, and confirming the advantages in terms of improved aerodynamic performance already demonstrated for 2D configurations.

  8. Environment of deposition of Clear Fork Formation: Yoakum County, Texas

    SciTech Connect

    Moore, B.K.

    1987-05-01

    The Clear Fork Formation is Permian (Leonardian) in age and constitutes a major oil-bearing unit in the Permian basin of west Texas. In Yoakum County, west Texas, the upper Clear Fork carbonates record a subtidal upward-shoaling sequence of deposition. A small bryozoan-algal patch reef is situated within these carbonates near the southern edge of the North Basin platform. The reef is completely dolomitized, but paramorphic replacement has facilitated a study of the paleoecology, lateral variations, and community succession within this buildup. Build-ups of this type are scarcely known in strata of Permian age. The reef was apparently founded on a coquina horizon at the base of the buildup. The reef apparently had a low-relief, dome-shaped morphology. The trapping and binding of sediment by bryozoa appear to have been the main constructional process. A significant role was also played by encrusting forams and the early precipitation of submarine cements, both of which added rigidity to the structure. The reef also contains a low-diversity community of other invertebrates. Algal constituents predominate at the basinward edge of the buildup. The reef was formed entirely subaqueously on a broad, relatively shallow tropical marine carbonate shelf environment. An understanding of the lithofacies distribution and paragenesis within this sequence will provide information on porosity variations and the nature and distribution of permeability barriers. Such information is useful in reservoir modeling studies and for secondary recovery techniques in shelf-edge carbonate reservoirs of this type.

  9. Middle Fork and Upper Salmon River Habitat Improvement Implementation Plan, FY 1988-1992.

    SciTech Connect

    Andrews, John; Everson, Larry B.

    1988-02-01

    This document presents an implementation plan for completing the phase II portion of the Middle Fork and Upper Salmon River Habitat Improvement Agreement. Underseeding of spawning adult salmon and steelhead, high instream sediment levels, a lack of habitat diversity in the form of overhanging riparian vegetation and edge, and barriers to both adult and juvenile anadromous fish migration were identified as the principal factors limiting anadromous fish production in the project area. Underseeding is being addressed in other projects sponsored and funded by the Bonneville Power Administration while this implementation plan lays out a schedule for resolving the other identified limiting factors. The primary goal of this program is to increase the quality and quantity of anadromous fish habitat (spring chinook and summer steelhead) with an emphasis on the survival of the wild stocks. This goal will be achieved by reducing the impact of sediment loading, improving riparian vegetation, eliminating passage barriers, and increasing habitat diversity. Meeting the above goal will provide off-site mitigation under the manadate of the pacific northwest electric power planning and conservation act of 1980. Project implementation will follow measures in the Northwest Power Council's Columbia River Fish and Wildlife Program. 9 refs., 3 figs., 5 tabs.

  10. INTEGRATED OUTCROP AND SUBSURFACE STUDIES OF THE INTERWELL ENVIRONMENT OF CARBONATE RESERVOIRS: CLEAR FORK (LEONARDIAN-AGE) RESERVOIRS, WEST TEXAS AND NEW MEXICO

    SciTech Connect

    F. Jerry Lucia

    2002-01-31

    This is the final report of the project ''Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonardian-Age) Reservoirs, West Texas and New Mexico'', Department of Energy contract no. DE-AC26-98BC15105 and is the third in a series of similar projects funded jointly by the U.S. Department of Energy and The University of Texas at Austin, Bureau of Economic Geology, Reservoir Characterization Research Laboratory for Carbonates. All three projects focus on the integration of outcrop and subsurface data for the purpose of developing improved methods for modeling petrophysical properties in the interwell environment. The first project, funded by contract no. DE-AC22-89BC14470, was a study of San Andres outcrops in the Algerita Escarpment, Guadalupe Mountains, Texas and New Mexico, and the Seminole San Andres reservoir, Permian Basin. This study established the basic concepts for constructing a reservoir model using sequence-stratigraphic principles and rock-fabric, petrophysical relationships. The second project, funded by contract no. DE-AC22-93BC14895, was a study of Grayburg outcrops in the Brokeoff Mountains, New Mexico, and the South Cowden Grayburg reservoir, Permian Basin. This study developed a sequence-stratigraphic succession for the Grayburg and improved methods for locating remaining hydrocarbons in carbonate ramp reservoirs. The current study is of the Clear Fork Group in Apache Canyon, Sierra Diablo Mountains, West Texas, and the South Wasson Clear Fork reservoir, Permian Basin. The focus was on scales of heterogeneity, imaging high- and low-permeability layers, and the impact of fractures on reservoir performance. In this study (1) the Clear Fork cycle stratigraphy is defined, (2) important scales of petrophysical variability are confirmed, (3) a unique rock-fabric, petrophysical relationship is defined, (4) a porosity method for correlating high-frequency cycles and defining rock-fabric flow layers

  11. Grand Forks - East Grand Forks Urban Water Resources Study. Energy conservation and recreation appendix, public involvement appendix. Final report

    SciTech Connect

    Not Available

    1981-07-01

    The goal of the Corps of Engineers Urban Study Program is to provide planning assistance to local interests in a variety of water resource areas. The St. Paul District conducted the Grand Forks-East Grand Forks (GF/EGF) Urban Water Resources Study which was a cooperative effort among local, state and federal agencies. Primary attention was given to flood control, water supply and wastewater management; supporting investigations addressed recreation and energy conservation. The recreation investigation consists of the leisure time analysis conducted in stage 2 of the urban study by the Heritage Conservation and Recreation Service, Department of the Interior. The leisure time analysis compared the study area's recreational needs to the available and planned facilities and identified unsatisfied needs. The thermography investigation was conducted in spring 1978 in response to the public's growing awareness of energy conservation and the Corps' desire to make the public aware of the urban study in a meaningful, useful fashion. The investigation consisted of aerial infrared photography, public displays of photographs, and distribution of information on energy saving practices. This investigation was a one-time effort with no plans for follow-up.

  12. South Fork Flathead Watershed Westslope Cutthroat Trout Conservation Program, Annual Report 2002.

    SciTech Connect

    Grisak, Grant; Marotz, Brian

    2003-06-01

    In 1999, Montana Fish, Wildlife & Parks (MFWP) began a program aimed at conserving the genetically pure populations of westslope cutthroat trout in the South Fork Flathead River drainage. The objective of this program is to eliminate all of the exotic and hybrid trout that threaten the genetically pure westslope cutthroat populations in the South Fork Flathead. The exotic and hybrid trout populations occur in several headwater lakes and their outflow streams. In 2001 MFWP released a draft environmental assessment, pursuant to the Montana Environmental Policy Act (MEPA), that addressed the use of motorized equipment to deliver personnel and materials to some of these lakes in the Bob Marshall and Great Bear Wildernesses (Grisak 2001). After a 30-day public comment period, MFWP determined that the complexity of issues was too great and warranted a more detailed analysis. These issues included transportation options for personnel, equipment and materials, the use of motorized equipment in wilderness, fish removal methods, fish stocking, and the status and distribution of amphibian populations in the project area. Because the program also involves the U.S. Forest Service (USFS) and Bonneville Power Administration (BPA), the environmental analysis needs to comply with the National Environmental Policy Act (NEPA). In October 2001, pursuant to NEPA, MFWP, along with the USFS and BPA initiated an environmental assessment to address these issues. In June 2002, the three agencies determined that the scope of these issues warranted an Environmental Impact Statement. This specialist report describes the logistical, technical and biological issues associated with this project and provides an analysis of options for fish removal, transportation and fish stocking. It further analyzes issues and concerns associated with amphibian populations and creating new domesticated stocks of westslope cutthroat trout. Finally, this document provides a description of each lake, the best

  13. Safeguarding genome stability: RASSF1A tumor suppressor regulates BRCA2 at stalled forks

    PubMed Central

    Pefani, Dafni Eleftheria; O'Neill, Eric

    2015-01-01

    While it has been widely established that defective fork restart after exposure to stress results in increased genomic instability, the importance of fork protection during stalling for safeguarding genomic integrity has recently been fully appreciated. BRCA2, Breast tumor suppressor, has dual functionality promoting not only DNA repair but also preventing DNA lesions at stalled forks. In response to replication stress, BRCA2 recruits RAD51 onto nascent DNA at stalled forks, protecting nascent DNA from nucleolitic cleavage. Phosphorylation of the BRCA2 C-terminal RAD51 binding site by CDK2 promotes RAD51 filament disassembly, leading to nucleolitic cleavage of newly synthesized DNA and compromised fork integrity. Recently we uncovered how the core Hippo pathway components RASSF1A, MST2 and LATS1 regulate CDK2 activity towards BRCA2, in response to fork stalling. In complex with LATS1, CDK2 exhibits reduced kinase activity which results in low levels of pBRCA2-S3291 and stable RAD51 filaments protecting nascent DNA from MRE11 cleavage. In the absence of the RASSF1A/MST2/LATS1/CDK2 pathway increased resection of newly synthesized DNA leads to chromosomal instability and malignant transformation. This function of RASSF1A in stalled replication fork protection adds to the role of RASSF1A as a tumor suppressor and builds up evidence for RASSF1A status and its prognostic and predictive value in cancer. PMID:25927241

  14. X-15 flight crew - Engle, Rushworth, McKay, Knight, Thompson, and Dana

    NASA Technical Reports Server (NTRS)

    1966-01-01

    The X-15 flight crew, left to right; Air Force Captain Joseph H. Engle, Air Force Major Robert A. Rushworth, NASA pilot John B. 'Jack' McKay, Air Force Major William J. 'Pete' Knight, NASA pilot Milton O. Thompson, and NASA pilot Bill Dana. These six pilots made 125 of the 199 total flights in the X-15. Rushworth made 34 flights (the most of any X-15 pilot); McKay flew 29 times; Engle, Knight, and Dana each flew 16 times; Thompson's total was 14. The X-15 was a rocket-powered aircraft 50 ft long with a wingspan of 22 ft. It was a missile-shaped vehicle with an unusual wedge-shaped vertical tail, thin stubby wings, and unique fairings that extended along the side of the fuselage. The X-15 weighed about 14,000 lb empty and approximately 34,000 lb at launch. The XLR-99 rocket engine, manufactured by Thiokol Chemical Corp., was pilot controlled and was capable of developing 57,000 lb of rated thrust (actual thrust reportedly climbed to 60,000 lb). North American Aviation built three X-15 aircraft for the program. The X-15 research aircraft was developed to provide in-flight information and data on aerodynamics, structures, flight controls, and the physiological aspects of high-speed, high-altitude flight. A follow-on program used the aircraft as a testbed to carry various scientific experiments beyond the Earth's atmosphere on a repeated basis. For flight in the dense air of the usable atmosphere, the X-15 used conventional aerodynamic controls such as rudder surfaces on the vertical stabilizers to control yaw and canted horizontal surfaces on the tail to control pitch when moving in synchronization or roll when moved differentially. For flight in the thin air outside of the appreciable Earth's atmosphere, the X-15 used a reaction control system. Hydrogen peroxide thrust rockets located on the nose of the aircraft provided pitch and yaw control. Those on the wings provided roll control. Because of the large fuel consumption, the X-15 was air launched from a B-52

  15. Carbon trace gases in lake and beaver pond ice near Thompson, Manitoba, Canada

    NASA Astrophysics Data System (ADS)

    Kuhlbusch, Thomas A. J.; Zepp, Richard G.

    1999-11-01

    Concentrations of CO2, CO, and CH4 were measured in beaver pond and lake ice in April 1996 near Thompson, Manitoba to derive information on possible impacts of ice melting on corresponding atmospheric trace gas concentrations. CH4 concentrations in beaver pond and lake ice ranged between 0.3-150 mmol m-3 and 3.1-56.2 μmol m-3, respectively. The corresponding CO concentrations showed no significant differences between the two lakes. They varied between 50 and 250 μmol m-3. These CO concentrations are some of the highest determined in any aquatic system. The differences in CH4 concentrations between lake and pond can be explained by the differences in production and microbial oxidation rates between the two systems. No explanation can be given for the similar CO concentrations. Supersaturation factors for CO were 660±130 and 630±330, and 65-35000 and 0.6-13 for CH4 in the ice of the beaver pond and Troy Lake, respectively. When digging into the beaver pond ice, a continuous flow of bubbles with 0.32±0.06 vol% CH4, 2.2±0.3 vol% CO2, and 482±98 ppb CO coming out of the slash ice for about 20-30 minutes was noticed. Wintertime flux estimates of CH4 and CO showed that they represent at minimum 6.4% and 2.2% of that of the summer. It has to be noted that these wintertime fluxes will mostly be released to the atmosphere during the time of snowmelt, thus a limited time period of weeks.

  16. Geomorphic Characterization of the Middle Fork Saline River: Garland, Perry, and Saline Counties, Arkansas

    USGS Publications Warehouse

    Pugh, Aaron L.; Garday, Thomas J.; Redman, Ronald

    2008-01-01

    This report was prepared to help address concerns raised by local residents, State, and Federal agencies about the current geomorphic conditions of the Middle Fork Saline River. Over the past 30 years the Middle Fork Saline River Basin has experienced a marked increase in urbanization. The report summarizes the Middle Fork?s current (2003) channel characteristics at nine stream reaches in the upper 91 square miles of the basin. Assessments at each study reach included comparing measured stream geometry dimensions (cross-sectional area, top width, and mean depth) at bankfull stage to regional hydraulic geometry curves for the Ouachita Mountains Physiographic Province of Arkansas and Oklahoma, evaluations of streambed materials and sinuosity, and classification of individual stream reach types. When compared to the Ouachita Mountains? regional hydraulic geometry curves for natural, stable, stream reaches, five of the nine study reaches had slightly smaller crosssectional areas, longer top widths, and shallower depths. Streambed material analysis indicates that the Middle Fork is a bedrock influenced, gravel dominated stream with lesser amounts of sand and cobbles. Slight increases in sinuosity from 1992 to 2002 at seven of the nine study reaches indicate a slight decrease in stream channel slope. Analyses of the Middle Fork?s hydraulic geometry and sinuosity indicate that the Middle Fork is currently overly wide and shallow, but is slowly adjusting towards a deeper, narrower hydraulic geometry. Using the Rosgen system of channel classification, the two upstream study reaches classified as B4c/1 stream types; which were moderately entrenched, riffle dominated channels, with infrequently spaced pools. The downstream seven study reaches classified as C4/1 stream types; which were slightly entrenched, meandering, gravel-dominated, riffle/ pool channels with well developed flood plains. Analyses of stream reach types suggest that the downstream reaches of the Middle Fork

  17. Oxbow Conservation Area; Middle Fork John Day River, Annual Report 2003-2004.

    SciTech Connect

    Cochran, Brian

    2004-02-01

    In early 2001, the Confederated Tribes of Warm Springs, through their John Day Basin Office, concluded the acquisition of the Oxbow Ranch, now know as the Oxbow Conservation Area (OCA). Under a memorandum of agreement with the Bonneville Power Administration (BPA), the Tribes are required to provided BPA an 'annual written report generally describing the real property interests in the Project, HEP analyses undertaken or in progress, and management activities undertaken or in progress'. The project during 2003 was crippled due to the aftermath of the BPA budget crisis. Some objectives were not completed during the first half of this contract because of limited funds in the 2003 fiscal year. The success of this property purchase can be seen on a daily basis. Water rights were utilized only in the early, high water season and only from diversion points with functional fish screens. After July 1, all of the OCA water rights were put instream. Riparian fences on the river, Ruby and Granite Boulder creeks continued to promote important vegetation to provide shade and bank stabilization. Hundreds of willow, dogwood, Douglas-fir, and cottonwood were planted along the Middle Fork John Day River. Livestock grazing on the property was carefully managed to ensure the protection of fish and wildlife habitat, while promoting meadow vigor and producing revenue for property taxes. Monitoring of property populations, resources, and management activities continued in 2003 to build a database for future management of this and other properties in the region.

  18. Improved Tuning Fork for Terahertz Quartz-Enhanced Photoacoustic Spectroscopy.

    PubMed

    Sampaolo, Angelo; Patimisco, Pietro; Giglio, Marilena; Vitiello, Miriam S; Beere, Harvey E; Ritchie, David A; Scamarcio, Gaetano; Tittel, Frank K; Spagnolo, Vincenzo

    2016-01-01

    We report on a quartz-enhanced photoacoustic (QEPAS) sensor for methanol (CH₃OH) detection employing a novel quartz tuning fork (QTF), specifically designed to enhance the QEPAS sensing performance in the terahertz (THz) spectral range. A discussion of the QTF properties in terms of resonance frequency, quality factor and acousto-electric transduction efficiency as a function of prong sizes and spacing between the QTF prongs is presented. The QTF was employed in a QEPAS sensor system using a 3.93 THz quantum cascade laser as the excitation source in resonance with a CH₃OH rotational absorption line located at 131.054 cm(-1). A minimum detection limit of 160 ppb in 30 s integration time, corresponding to a normalized noise equivalent absorption NNEA = 3.75 × 10(-11) cm(-1)W/Hz(½), was achieved, representing a nearly one-order-of-magnitude improvement with respect to previous reports. PMID:27023552

  19. Mechanism of asymmetric polymerase assembly at the eukaryotic replication fork.

    PubMed

    Georgescu, Roxana E; Langston, Lance; Yao, Nina Y; Yurieva, Olga; Zhang, Dan; Finkelstein, Jeff; Agarwal, Tani; O'Donnell, Mike E

    2014-08-01

    Eukaryotes use distinct polymerases for leading- and lagging-strand replication, but how they target their respective strands is uncertain. We reconstituted Saccharomyces cerevisiae replication forks and found that CMG helicase selects polymerase (Pol) ɛ to the exclusion of Pol δ on the leading strand. Even if Pol δ assembles on the leading strand, Pol ɛ rapidly replaces it. Pol δ-PCNA is distributive with CMG, in contrast to its high stability on primed ssDNA. Hence CMG will not stabilize Pol δ, instead leaving the leading strand accessible for Pol ɛ and stabilizing Pol ɛ. Comparison of Pol ɛ and Pol δ on a lagging-strand model DNA reveals the opposite. Pol δ dominates over excess Pol ɛ on PCNA-primed ssDNA. Thus, PCNA strongly favors Pol δ over Pol ɛ on the lagging strand, but CMG over-rides and flips this balance in favor of Pol ɛ on the leading strand. PMID:24997598

  20. Mechanism of asymmetric polymerase assembly at the eukaryotic replication fork

    PubMed Central

    Georgescu, Roxana E; Langston, Lance; Yao, Nina Y; Yurieva, Olga; Zhang, Dan; Finkelstein, Jeff; Agarwal, Tani; O’Donnell, Mike E

    2015-01-01

    Eukaryotes use distinct polymerases for leading- and lagging-strand replication, but how they target their respective strands is uncertain. We reconstituted Saccharomyces cerevisiae replication forks and found that CMG helicase selects polymerase (Pol) ε to the exclusion of Pol δ on the leading strand. Even if Pol δ assembles on the leading strand, Pol ε rapidly replaces it. Pol δ–PCNA is distributive with CMG, in contrast to its high stability on primed ssDNA. Hence CMG will not stabilize Pol δ, instead leaving the leading strand accessible for Pol ε and stabilizing Pol ε. Comparison of Pol ε and Pol δ on a lagging-strand model DNA reveals the opposite. Pol δ dominates over excess Pol ε on PCNA-primed ssDNA. Thus, PCNA strongly favors Pol δ over Pol ε on the lagging strand, but CMG over-rides and flips this balance in favor of Pol ε on the leading strand. PMID:24997598

  1. Improved Tuning Fork for Terahertz Quartz-Enhanced Photoacoustic Spectroscopy

    PubMed Central

    Sampaolo, Angelo; Patimisco, Pietro; Giglio, Marilena; Vitiello, Miriam S.; Beere, Harvey E.; Ritchie, David A.; Scamarcio, Gaetano; Tittel, Frank K.; Spagnolo, Vincenzo

    2016-01-01

    We report on a quartz-enhanced photoacoustic (QEPAS) sensor for methanol (CH3OH) detection employing a novel quartz tuning fork (QTF), specifically designed to enhance the QEPAS sensing performance in the terahertz (THz) spectral range. A discussion of the QTF properties in terms of resonance frequency, quality factor and acousto-electric transduction efficiency as a function of prong sizes and spacing between the QTF prongs is presented. The QTF was employed in a QEPAS sensor system using a 3.93 THz quantum cascade laser as the excitation source in resonance with a CH3OH rotational absorption line located at 131.054 cm−1. A minimum detection limit of 160 ppb in 30 s integration time, corresponding to a normalized noise equivalent absorption NNEA = 3.75 × 10−11 cm−1W/Hz½, was achieved, representing a nearly one-order-of-magnitude improvement with respect to previous reports. PMID:27023552

  2. Geochemical map of the North Fork John Day River Roadless Area, Grant County, Oregon

    USGS Publications Warehouse

    Evans, James G.

    1986-01-01

    The North Fork John Day River Roadless Area comprised 21,210 acres in the Umatilla and Wallowa-Whitman National Forests, Grant County, Oregon, about 30 miles northwest of Baker, Oregon. The irregularly shaped area extends for about 1 mile on both sides of a 25-mile segment of the North Fork John Day River from Big Creek on the west to North Fork John Day Campground on the east. Most of the roadless area is in the northern half of the Desolation Butte 15-minute quadrangle. The eastern end of the area is in parts of the Granite and Trout Meadows 7½-minute quadrangles.

  3. Q-factor optimization of a tuning-fork/fiber sensor for shear-force detection

    NASA Astrophysics Data System (ADS)

    Morville, Jérôme; Liu, Jinquan; Callegari, Andrea; Chergui, Majed

    2005-02-01

    We present the results of an experimental and theoretical study on the optimum design of shear-force sensors, used in scanning probe microscopes. We have optimized a configuration consisting of a tuning-fork/fiber-tip assembly, achieving quality factors (Q) exceeding 8000, and have presented a theoretical analysis of the design wherein the force holding the fiber and fork in contact is provided solely by elastic mechanical deformation, which allows full control of the performance of the system. On this basis, we constructed a high-quality-factor configuration with the fiber glued onto the tuning fork.

  4. On cavitation in liquid helium in a flow due to a vibrating quartz fork

    NASA Astrophysics Data System (ADS)

    Blažková, M.; Schmoranzer, D.; Skrbek, L.

    2008-04-01

    Cavitation in normal and superfluid liquid He4 at saturated vapor pressure and slightly elevated pressures has been experimentally studied in a flow due to quartz forks vibrating at high amplitudes. Above the temperature- and pressure-dependent critical velocity, heterogeneous cavitation is observed both visually and electrically, as a breakdown of the resonance response of the fork. We compare our results with available experimental and discuss them using existing theoretical models. In particular, we show that thermal effects leading to local overheating of the vicinity of the fork have to be taken into account, especially in normal liquid He4.

  5. A tuning fork gyroscope with compensated imbalance signal

    NASA Astrophysics Data System (ADS)

    Arnold, E.; Nuscheler, F.

    2007-05-01

    This paper is presenting a robust gyroscope sensor with an electrical and mechanical self-test option and the ability to suppress the quadrature error. The presented sensor is based on a tuning-fork working principle. The mechanical part is assembled in bulk-technology produced with a wet etching process. The two detection elements are manufactured with a standard CMOS-process and the material of the two thin-film actuators is AlN (aluminium-nitrid). The two actuators can be controlled independently from each other. Two electronic PCB's were developed for actuating and measurement. One is including the analogue signal path; the second PCB is the digital electronics consisting of a FPGA and other peripherals. The tuning fork is actuated in a primary oscillation mode also called drive mode. For keeping the oscillation in resonance, a digital PLL is used in a forced feedback loop. To have a constant energy in the drive mode an Amplitude-Gain-Control (AGC) is implemented. An appearing angular rate causes the corriolis-force which is actuating secondary oscillation, also called detection mode. The amplitude of this oscillation is proportional to the angular rate. The signal has a component resulting from the mechanical imbalance. To separate these two signal parts from each other a synchronous demodulator followed by a digital filter chain has been developed. To achieve the maximum suppression of the imbalance signal a control-loop is used to shift the phases of the two actuation signals. This creates an additional force that compensates the movement as a result of the mechanical imbalance. With the implementation of this control loop the performance of the sensor was increased. An enhanced temperature stability over operation was achieved with the means of this compensation.

  6. HENRY'S FORK AND SNAKE RIVER BASIN, IDAHO - WATER QUALITY REPORT, 1973

    EPA Science Inventory

    Reported problems in the Henrys Fork and Snake River Basin (17040202, 17040203, 17040201) include bacteria levels exceeding water quality standards, dissolved oxygen standards violations, and excessive algal blooms resulting in aesthetic problems and contributing to DO depression...

  7. Stalled DNA Replication Forks at the Endogenous GAA Repeats Drive Repeat Expansion in Friedreich's Ataxia Cells.

    PubMed

    Gerhardt, Jeannine; Bhalla, Angela D; Butler, Jill Sergesketter; Puckett, James W; Dervan, Peter B; Rosenwaks, Zev; Napierala, Marek

    2016-08-01

    Friedreich's ataxia (FRDA) is caused by the expansion of GAA repeats located in the Frataxin (FXN) gene. The GAA repeats continue to expand in FRDA patients, aggravating symptoms and contributing to disease progression. The mechanism leading to repeat expansion and decreased FXN transcription remains unclear. Using single-molecule analysis of replicated DNA, we detected that expanded GAA repeats present a substantial obstacle for the replication machinery at the FXN locus in FRDA cells. Furthermore, aberrant origin activation and lack of a proper stress response to rescue the stalled forks in FRDA cells cause an increase in 3'-5' progressing forks, which could enhance repeat expansion and hinder FXN transcription by head-on collision with RNA polymerases. Treatment of FRDA cells with GAA-specific polyamides rescues DNA replication fork stalling and alleviates expansion of the GAA repeats, implicating DNA triplexes as a replication impediment and suggesting that fork stalling might be a therapeutic target for FRDA. PMID:27425605

  8. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame... significantly limits the steering angle over which the wheel can be turned. Sidewalk bicycles are exempt...

  9. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame... significantly limits the steering angle over which the wheel can be turned. Sidewalk bicycles are exempt...

  10. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame... significantly limits the steering angle over which the wheel can be turned. Sidewalk bicycles are exempt...

  11. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame... significantly limits the steering angle over which the wheel can be turned. Sidewalk bicycles are exempt...

  12. Evaluation of the Doraiswamy-Thompson winter wheat crop calendar model incorporating a modified spring restart sequence

    NASA Technical Reports Server (NTRS)

    Taylor, T. W.; Ravet, F. W.; Smika, D. (Principal Investigator)

    1981-01-01

    The Robertson phenology was used to provide growth stage information to a wheat stress indicator mode. A stress indicator model demands two acurate predictions from a crop calendar: date of spring growth initiation; and crop calendar stage at growth initiation. Several approaches for restarting the Robertson phenology model at spring growth initiation were studied. Although best results were obtained with a solar thermal unit method, an alternate approach which indicates soil temperature as the controlling parameter for spring growth initiation was selected and tested. The modified model (Doraiswamy-Thompson) is compared to LACIE-Robertson model predictions.

  13. Horvitz-Thompson survey sample methods for estimating large-scale animal abundance

    USGS Publications Warehouse

    Samuel, M.D.; Garton, E.O.

    1994-01-01

    Large-scale surveys to estimate animal abundance can be useful for monitoring population status and trends, for measuring responses to management or environmental alterations, and for testing ecological hypotheses about abundance. However, large-scale surveys may be expensive and logistically complex. To ensure resources are not wasted on unattainable targets, the goals and uses of each survey should be specified carefully and alternative methods for addressing these objectives always should be considered. During survey design, the impoflance of each survey error component (spatial design, propofiion of detected animals, precision in detection) should be considered carefully to produce a complete statistically based survey. Failure to address these three survey components may produce population estimates that are inaccurate (biased low), have unrealistic precision (too precise) and do not satisfactorily meet the survey objectives. Optimum survey design requires trade-offs in these sources of error relative to the costs of sampling plots and detecting animals on plots, considerations that are specific to the spatial logistics and survey methods. The Horvitz-Thompson estimators provide a comprehensive framework for considering all three survey components during the design and analysis of large-scale wildlife surveys. Problems of spatial and temporal (especially survey to survey) heterogeneity in detection probabilities have received little consideration, but failure to account for heterogeneity produces biased population estimates. The goal of producing unbiased population estimates is in conflict with the increased variation from heterogeneous detection in the population estimate. One solution to this conflict is to use an MSE-based approach to achieve a balance between bias reduction and increased variation. Further research is needed to develop methods that address spatial heterogeneity in detection, evaluate the effects of temporal heterogeneity on survey

  14. Probing Liquid ^4He with Quartz Tuning Forks Using a Novel Multifrequency Lock-in Technique

    NASA Astrophysics Data System (ADS)

    Bradley, D. I.; Haley, R. P.; Kafanov, S.; Noble, M. T.; Pickett, G. R.; Tsepelin, V.; Vonka, J.; Wilcox, T.

    2016-09-01

    We report on a novel technique to measure quartz tuning forks, and possibly other vibrating objects, in a quantum fluid using a multifrequency lock-in amplifier. The multifrequency technique allows to measure the resonance curve of a vibrating object much faster than a conventional single frequency lock-in amplifier technique. Forks with resonance frequencies of 12 kHz and 16 kHz were excited and measured electro-mechanically either at a single frequency or at up to 40 different frequencies simultaneously around the same mechanical mode. The response of each fork was identical for both methods and validates the use of the multifrequency lock-in technique to probe properties of liquid helium at low fork velocities. Using both methods we measured the resonance frequency and drag of two 25-μ m-wide quartz tuning forks immersed in liquid ^4He in the temperature range from 4.2 K to 1.5 K at saturated vapour pressure. The damping and shift of resonance frequency experienced by both tuning forks at low velocities are well described by hydrodynamic contributions in the framework of the two-fluid model. The sensitivity of the 25-μ m-wide tuning forks is larger compared to similar 75-μ m-wide forks and in combination with the faster multifrequency lock-in technique could be used to improve thermometry in liquid ^4He. The multifrequency technique could also be used for studies of the onset of non-linear phenomena such as quantum turbulence and cavitation in superfluids.

  15. Probing Liquid ^4 He with Quartz Tuning Forks Using a Novel Multifrequency Lock-in Technique

    NASA Astrophysics Data System (ADS)

    Bradley, D. I.; Haley, R. P.; Kafanov, S.; Noble, M. T.; Pickett, G. R.; Tsepelin, V.; Vonka, J.; Wilcox, T.

    2016-06-01

    We report on a novel technique to measure quartz tuning forks, and possibly other vibrating objects, in a quantum fluid using a multifrequency lock-in amplifier. The multifrequency technique allows to measure the resonance curve of a vibrating object much faster than a conventional single frequency lock-in amplifier technique. Forks with resonance frequencies of 12 kHz and 16 kHz were excited and measured electro-mechanically either at a single frequency or at up to 40 different frequencies simultaneously around the same mechanical mode. The response of each fork was identical for both methods and validates the use of the multifrequency lock-in technique to probe properties of liquid helium at low fork velocities. Using both methods we measured the resonance frequency and drag of two 25-μ m-wide quartz tuning forks immersed in liquid ^4 He in the temperature range from 4.2 K to 1.5 K at saturated vapour pressure. The damping and shift of resonance frequency experienced by both tuning forks at low velocities are well described by hydrodynamic contributions in the framework of the two-fluid model. The sensitivity of the 25-μ m-wide tuning forks is larger compared to similar 75-μ m-wide forks and in combination with the faster multifrequency lock-in technique could be used to improve thermometry in liquid ^4 He. The multifrequency technique could also be used for studies of the onset of non-linear phenomena such as quantum turbulence and cavitation in superfluids.

  16. The Sound Field around a Tuning Fork and the Role of a Resonance Box

    ERIC Educational Resources Information Center

    Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

    2015-01-01

    Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: (1) Why is a bare tuning fork such a weak emitter of sound? (2) What is the role of the…

  17. Regression of Replication Forks Stalled by Leading-strand Template Damage

    PubMed Central

    Gupta, Sankalp; Yeeles, Joseph T. P.; Marians, Kenneth J.

    2014-01-01

    Stalled replication forks are sites of chromosome breakage and the formation of toxic recombination intermediates that undermine genomic stability. Thus, replication fork repair and reactivation are essential processes. Among the many models of replication fork reactivation is one that invokes fork regression catalyzed by the strand exchange protein RecA as an intermediate in the processing of the stalled fork. We have investigated the replication fork regression activity of RecA using a reconstituted DNA replication system where the replisome is stalled by collision with leading-strand template damage. We find that RecA is unable to regress the stalled fork in the presence of the replisome and SSB. If the replication proteins are removed from the stalled fork, RecA will catalyze net regression as long as the Okazaki fragments are sealed. RecA-generated Holliday junctions can be detected by RuvC cleavage, although this is not a robust reaction. On the other hand, extensive branch migration by RecA, where a completely unwound product consisting of the paired nascent leading and lagging strands is produced, is observed under conditions where RuvC activity is suppressed. This branch migration reaction is inhibited by SSB, possibly accounting for the failure of RecA to generate products in the presence of the replication proteins. Interestingly, we find that the RecA-RuvC reaction is supported to differing extents, depending on the template damage; templates carrying a cyclopyrimidine dimer elicit more RecA-RuvC product than those carrying a synthetic abasic site. This difference could be ascribed to a higher affinity of RecA binding to DNAs carrying a thymidine dimer than to those with an abasic site. PMID:25138217

  18. Jay L. King, Joseph D. Huxman, and Orion D. Billeter Assist Pilot Milt Thompson into the M2-F2 Attac

    NASA Technical Reports Server (NTRS)

    1966-01-01

    NASA research pilot Milt Thompson is helped into the cockpit of the M2-F2 lifting body research aircraft at NASA's Flight Research Center (now the Dryden Flight Research Center). The M2-F2 is attached to a wing pylon under the wing of NASA's B-52 mothership. The flight was a captive flight with the pilot on-board. Milt Thompson flew in the lifting body throughout the flight, but it was never dropped from the mothership. NASA B-52, Tail Number 008, is an air launch carrier aircraft, 'mothership,' as well as a research aircraft platform that has been used on a variety of research projects. The aircraft, a 'B' model built in 1952 and first flown on June 11, 1955, is the oldest B-52 in flying status and has been used on some of the most significant research projects in aerospace history. Some of the significant projects supported by B-52 008 include the X-15, the lifting bodies, HiMAT (highly maneuverable aircraft technology), Pegasus, validation of parachute systems developed for the space shuttle program (solid-rocket-booster recovery system and the orbiter drag chute system), and the X-38. The B-52 served as the launch vehicle on 106 X-15 flights and flew a total of 159 captive-carry and launch missions in support of that program from June 1959 to October 1968. Information gained from the highly successful X-15 program contributed to the Mercury, Gemini, and Apollo human spaceflight programs as well as space shuttle development. Between 1966 and 1975, the B-52 served as the launch aircraft for 127 of the 144 wingless lifting body flights. In the 1970s and 1980s, the B-52 was the launch aircraft for several aircraft at what is now the Dryden Flight Research Center, Edwards, California, to study spin-stall, high-angle-of attack, and maneuvering characteristics. These included the 3/8-scale F-15/spin research vehicle (SRV), the HiMAT (Highly Maneuverable Aircraft Technology) research vehicle, and the DAST (drones for aerodynamic and structural testing). The aircraft

  19. DNA2 drives processing and restart of reversed replication forks in human cells

    PubMed Central

    Thangavel, Saravanabhavan; Berti, Matteo; Levikova, Maryna; Pinto, Cosimo; Gomathinayagam, Shivasankari; Vujanovic, Marko; Zellweger, Ralph; Moore, Hayley; Lee, Eu Han; Hendrickson, Eric A.; Cejka, Petr; Stewart, Sheila; Lopes, Massimo

    2015-01-01

    Accurate processing of stalled or damaged DNA replication forks is paramount to genomic integrity and recent work points to replication fork reversal and restart as a central mechanism to ensuring high-fidelity DNA replication. Here, we identify a novel DNA2- and WRN-dependent mechanism of reversed replication fork processing and restart after prolonged genotoxic stress. The human DNA2 nuclease and WRN ATPase activities functionally interact to degrade reversed replication forks with a 5′-to-3′ polarity and promote replication restart, thus preventing aberrant processing of unresolved replication intermediates. Unexpectedly, EXO1, MRE11, and CtIP are not involved in the same mechanism of reversed fork processing, whereas human RECQ1 limits DNA2 activity by preventing extensive nascent strand degradation. RAD51 depletion antagonizes this mechanism, presumably by preventing reversed fork formation. These studies define a new mechanism for maintaining genome integrity tightly controlled by specific nucleolytic activities and central homologous recombination factors. PMID:25733713

  20. Checkpoint-dependent RNR induction promotes fork restart after replicative stress.

    PubMed

    Morafraile, Esther C; Diffley, John F X; Tercero, José Antonio; Segurado, Mónica

    2015-01-01

    The checkpoint kinase Rad53 is crucial to regulate DNA replication in the presence of replicative stress. Under conditions that interfere with the progression of replication forks, Rad53 prevents Exo1-dependent fork degradation. However, although EXO1 deletion avoids fork degradation in rad53 mutants, it does not suppress their sensitivity to the ribonucleotide reductase (RNR) inhibitor hydroxyurea (HU). In this case, the inability to restart stalled forks is likely to account for the lethality of rad53 mutant cells after replication blocks. Here we show that Rad53 regulates replication restart through the checkpoint-dependent transcriptional response, and more specifically, through RNR induction. Thus, in addition to preventing fork degradation, Rad53 prevents cell death in the presence of HU by regulating RNR-expression and localization. When RNR is induced in the absence of Exo1 and RNR negative regulators, cell viability of rad53 mutants treated with HU is increased and the ability of replication forks to restart after replicative stress is restored. PMID:25601385

  1. Checkpoint-dependent RNR induction promotes fork restart after replicative stress

    PubMed Central

    Morafraile, Esther C.; Diffley, John F. X.; Tercero, José Antonio; Segurado, Mónica

    2015-01-01

    The checkpoint kinase Rad53 is crucial to regulate DNA replication in the presence of replicative stress. Under conditions that interfere with the progression of replication forks, Rad53 prevents Exo1-dependent fork degradation. However, although EXO1 deletion avoids fork degradation in rad53 mutants, it does not suppress their sensitivity to the ribonucleotide reductase (RNR) inhibitor hydroxyurea (HU). In this case, the inability to restart stalled forks is likely to account for the lethality of rad53 mutant cells after replication blocks. Here we show that Rad53 regulates replication restart through the checkpoint-dependent transcriptional response, and more specifically, through RNR induction. Thus, in addition to preventing fork degradation, Rad53 prevents cell death in the presence of HU by regulating RNR-expression and localization. When RNR is induced in the absence of Exo1 and RNR negative regulators, cell viability of rad53 mutants treated with HU is increased and the ability of replication forks to restart after replicative stress is restored. PMID:25601385

  2. Restriction of Replication Fork Regression Activities by a Conserved SMC Complex

    PubMed Central

    Xue, Xiaoyu; Choi, Koyi; Bonner, Jaclyn; Chiba, Tamara; Kwon, Youngho; Xu, Yuanyuan; Sanchez, Humberto; Wyman, Claire; Niu, Hengyao; Zhao, Xiaolan; Sung, Patrick

    2014-01-01

    SUMMARY Conserved, multi-tasking DNA helicases mediate diverse DNA transactions and are relevant for human disease pathogenesis. These helicases and their regulation help maintain genome stability during DNA replication and repair. We show that the structural maintenance of chromosome complex Smc5-Smc6 restrains the replication fork regression activity of Mph1 helicase, but not its D-loop disruptive activity. This regulatory mechanism enables flexibility in replication fork repair without interfering with DNA break repair. In vitro studies find that Smc5-Smc6 binds to a Mph1 region required for efficient fork regression, preventing assembly of Mph1 oligomers at the junction of DNA forks. In vivo impairment of this regulatory mechanism compensates for the inactivation of another fork regression helicase and increases reliance on joint DNA structure removal or avoidance. Our findings provide molecular insights into replication fork repair regulation and uncover a role of Smc5-Smc6 in directing Mph1 activity towards a specific biochemical outcome. PMID:25439736

  3. RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells.

    PubMed

    Ragland, Ryan L; Patel, Sima; Rivard, Rebecca S; Smith, Kevin; Peters, Ashley A; Bielinsky, Anja-Katrin; Brown, Eric J

    2013-10-15

    The ATR-CHK1 axis stabilizes stalled replication forks and prevents their collapse into DNA double-strand breaks (DSBs). Here, we show that fork collapse in Atr-deleted cells is mediated through the combined effects the sumo targeted E3-ubiquitin ligase RNF4 and activation of the AURKA-PLK1 pathway. As indicated previously, Atr-deleted cells exhibited a decreased ability to restart DNA replication following fork stalling in comparison with control cells. However, suppression of RNF4, AURKA, or PLK1 returned the reinitiation of replication in Atr-deleted cells to near wild-type levels. In RNF4-depleted cells, this rescue directly correlated with the persistence of sumoylation of chromatin-bound factors. Notably, RNF4 repression substantially suppressed the accumulation of DSBs in ATR-deficient cells, and this decrease in breaks was enhanced by concomitant inhibition of PLK1. DSBs resulting from ATR inhibition were also observed to be dependent on the endonuclease scaffold protein SLX4, suggesting that RNF4 and PLK1 either help activate the SLX4 complex or make DNA replication fork structures accessible for subsequent SLX4-dependent cleavage. Thus, replication fork collapse following ATR inhibition is a multistep process that disrupts replisome function and permits cleavage of the replication fork. PMID:24142876

  4. Effects of Engineered Log Jams on Channel Morphology, Middle Fork of the John Day River, Oregon

    NASA Astrophysics Data System (ADS)

    Duffin, J.; McDowell, P. F.

    2015-12-01

    Engineered log jams (ELJs) were constructed on the Middle Fork of the John Day River in eastern Oregon as part of a large river restoration project. These log structures were designed to address many of the restoration goals including creating scour pools, creating and maintaining a sinuous river planform, providing fish cover, and increasing complexity of fish habitat. This study uses geomorphic change detection techniques to monitor topographic change under and around the 26 log structures in two different river reaches over a six to seven year period. This study finds that the ELJs are remaining stable within the river, even following a large flood in 2011. While some pools are shallowing or shifting laterally slightly, in general they are maintaining deep pool habitat. The study provides insight into which log structure variables are most related to the patterns and amounts of aggradation and degradation. Analysis suggests that volume of aggradation is related to both porosity and volume of the log structures, while the area of aggradation was correlated with porosity, as well as, the proportion of logs within bankfull. Understanding the geomorphic changes to the riverbed in response to the placement of the ELJs can influence the design and future effectiveness of ELJs.

  5. Apollo Project

    NASA Technical Reports Server (NTRS)

    1966-01-01

    From Spaceflight Revolution: 'Top NASA officials listen to a LOPO briefing at Langley in December 1966. Sitting to the far right with his hand on his chin is Floyd Thompson. To the left sits Dr. George Mueller, NASA associate administrator for Manned Space Flight. On the wall is a diagram of the sites selected for the 'concentrated mission.' 'The most fundamental issue in the pre-mission planning for Lunar Orbiter was how the moon was to be photographed. Would the photography be 'concentrated' on a predetermined single target, or would it be 'distributed' over several selected targets across the moon's surface? On the answer to this basic question depended the successful integration of the entire mission plan for Lunar Orbiter.' The Lunar Orbiter Project made systematic photographic maps of the lunar landing sites. Published in James R. Hansen, Spaceflight Revolution: NASA Langley Research Center From Sputnik to Apollo, (Washington: NASA, 1995), p. 337.

  6. Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork.

    PubMed

    Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

    2015-06-01

    A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr(1-x)Ti(x))O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever. PMID:26133877

  7. Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork

    NASA Astrophysics Data System (ADS)

    Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

    2015-06-01

    A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr1-xTix)O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever.

  8. Changes in growth, photosynthetic activities, biochemical parameters and amino acid profile of Thompson Seedless grapes (Vitis vinifera L.).

    PubMed

    Somkuwar, R G; Bahetwar, Anita; Khan, I; Satisha, J; Ramteke, S D; Itroutwar, Prerna; Bhongale, Aarti; Oulkar, Dashrath

    2014-11-01

    The study on photosynthetic activity and biochemical parameters in Thompson Seedless grapes grafted on Dog Ridge rootstock and its impact on growth, yield and amino acid profile at various stages of berry development was conducted during the year 2012-2013. Leaf and berry samples from ten year old vines of Thompson Seedless were collected at different growth and berry developmental stages. The analysis showed difference in photosynthetic activity, biochemical parameters and amino acid status with the changes in berry development stage. Higher photosynthetic rate of 17.39 umol cm(-2) s(-1) was recorded during 3-4mm berry size and the lowest (10.08 umol cm(-2) s(-1)) was recorded during the veraison stage. The photosynthetic activity showed gradual decrease with the onset of harvest while the different biochemical parameters showed increase and decrease from one stage to another in both berry and leaves. Changes in photosynthetic activity and biochemical parameters thereby affected the growth, yield and amino acid content of the berry. Positive correlation of leaf area and photosynthetic rate was recorded during the period of study. Reducing sugar (352.25 mg g(-1)) and total carbohydrate (132.52 mg g(-1)) was more in berries as compared to leaf. Amino acid profile showed variations in different stages of berry development. Marked variations in photosynthetic as well as biochemical and amino acid content at various berry development stages was recorded and thereby its cumulative effect on the development of fruit quality. PMID:25522520

  9. Evaluating the effectiveness of floodplain restoration on the North Fork John Day River, Northeast Oregon, USA

    NASA Astrophysics Data System (ADS)

    Clifton, C. F.; Blanton, P.; Long, W.; Walterman, M. T.; McDowell, P. F.; Maus, P.

    2007-12-01

    Over the last decade hundreds of river restoration projects intended to maintain, protect, and restore watersheds, rivers, and habitat for native species in the Pacific Northwest have been implemented. By some counts, investment in watershed restoration exceeds hundreds of millions of dollars annually yet the effectiveness of these efforts remains an elusive question (Roni, 2005). Remote sensing and GIS technologies show great promise for large-scale river monitoring, however most natural resource organizations who implement these projects have limited budget and staff and would benefit from simple, low cost monitoring techniques that use readily available imagery. We used 1:24000 digitized orthorectified resource imagery from 1995, and National Agriculture Imagery Program (NAIP) digital orthophotography from 2005 to assess the effectiveness of floodplain restoration on a 16 km reach of the North Fork John Day River. Between 1993 and 1997 this section was restored by mechanically removing, reshaping, and revegetating cobble-boulder tailings piles left from dredge mining. The project was intended to directly improve floodplain function (i.e. inundation, riparian habitat) and indirectly improve instream habitat (pools, spawning) by reconnecting the active river channel with a reconstructed floodplain surface. Project effectiveness was not well documented initially in terms of quantifying floodplain functional area improvement or channel condition and response at the river-reach scale. Our objectives were to field-verify remote sensing measurements of response variables to test the applicability of available remote sensing imagery for project effectiveness monitoring, and to quantify adjustment in river response variables, using a "before-after" case study approach. Bracketing restoration activities with 1995 and 2000 imagery, we developed and tested methods for acquisition and processing of digital imagery and identified a core set of response variables to sample

  10. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils

    SciTech Connect

    1994-09-01

    The Lower East Fork Poplar Creek (LEFPC) extends fourteen (14) miles through Oak Ridge, TN. The Creek sediments and surrounding floodplain soils are contaminated with mercury compounds. This project involved a comprehensive pilot demonstration on thermal desorption of these soils to validate the feasibility of the remedial technology which had been identified in previous studies. Thermal desorption is a technology that utilizes heating or drying of soils to induce volatilization of contaminants. These contaminants are then vaporized and either incinerated or condensed in the second stage of desorption. Mercury (Hg), which was the principal contaminate of concern, was collected by condensers in a vapor collection system. This type of system insured that the toxic mercury vapors did not escape to the atmosphere.

  11. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    SciTech Connect

    ADAMS, S.M.; BEATY, T.W.; BRANDT, C.C.; CHRISTENSEN, S.W.; CICERONE, D.S.

    1998-09-09

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  12. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    SciTech Connect

    ADAMS, S.M.; ASHWOOD, T.L.; BEATY, T.W.; BRANDT, C.C.

    1997-10-24

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y- 12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  13. Water resources of the Little Fork River watershed, northeastern Minnesota

    USGS Publications Warehouse

    Helgesen, John O.; Lindholm, Gerald F.; Ericson, Donald W.

    1976-01-01

    The Little Fork River watershed is one of 39 watershed units designated by the Minnesota Department of Natural Resources for evaluation of the State 's water resources. Included is an appraisal of the occurrence, quantity, quality, and availability of ground and surface waters. Water resources are not intensively developed anywhere in the watershed. Essentially all water used is withdrawn from ground-water sources, mainly glacial drift, which ranges from 0 to over 200 feet (61 meters) in thickness. Buried sand and gravel in the drift is the most favorable source for development. Most ground water is of the calcium or calcium magnesium bicarbonate type. The degree of mineralization generally increases downgradient in the flow system. Ground water is commonly very hard and high in iron and manganese. Lakes and wetlands have a natural regulating effect on streamflow. Water in streams is of the calcium bicarbonate type. The amount of mineralization reflects surficial geology, being greatest in streams draining glacial-lake sediments and least in streams draining areas of sand lakes. Color and iron concentration in stream waters generally exceed recommended domestic consumption limits.

  14. Biological monitoring program for East Fork Poplar Creek

    SciTech Connect

    Adams, S.M.; Ashwood, T.L.; Beaty, T.W.; Brandt, C.C.; Christensen, S.W.; Cicerone, D.S.; Greeley, M.S. Jr.; Hill, W.R.; Kszos, L.S.

    1997-04-18

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  15. X-15 test pilots - Engle, Rushworth, McKay, Knight, Thompson, and Dana

    NASA Technical Reports Server (NTRS)

    1966-01-01

    The X-15 flight crew, left to right; Air Force Captain Joseph H. Engle, Air Force Major Robert A. Rushworth, NASA pilot John B. 'Jack' McKay, Air Force pilot William J. 'Pete' Knight, NASA pilot Milton O. Thompson, and NASA pilot Bill Dana. of their 125 X-15 flights, 8 were above the 50 miles that constituted the Air Force's definition of the beginning of space (Engle 3, Dana 2, Rushworth, Knight, and McKay one each). NASA used the international definition of space as beginning at 62 miles above the earth. The X-15 was a rocket-powered aircraft 50 ft long with a wingspan of 22 ft. It was a missile-shaped vehicle with an unusual wedge-shaped vertical tail, thin stubby wings, and unique fairings that extended along the side of the fuselage. The X-15 weighed about 14,000 lb empty and approximately 34,000 lb at launch. The XLR-99 rocket engine, manufactured by Thiokol Chemical Corp., was pilot controlled and was capable of developing 57,000 lb of rated thrust (actual thrust reportedly climbed to 60,000 lb). North American Aviation built three X-15 aircraft for the program. The X-15 research aircraft was developed to provide in-flight information and data on aerodynamics, structures, flight controls, and the physiological aspects of high-speed, high-altitude flight. A follow-on program used the aircraft as a testbed to carry various scientific experiments beyond the Earth's atmosphere on a repeated basis. For flight in the dense air of the usable atmosphere, the X-15 used conventional aerodynamic controls such as rudder surfaces on the vertical stabilizers to control yaw and canted horizontal surfaces on the tail to control pitch when moving in synchronization or roll when moved differentially. For flight in the thin air outside of the appreciable Earth's atmosphere, the X-15 used a reaction control system. Hydrogen peroxide thrust rockets located on the nose of the aircraft provided pitch and yaw control. Those on the wings provided roll control. Because of the large

  16. Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway.

    PubMed

    Calì, Federica; Bharti, Sanjay Kumar; Perna, Roberta Di; Brosh, Robert M; Pisani, Francesca M

    2016-01-29

    We present evidence that Tim establishes a physical and functional interaction with DDX11, a super-family 2 iron-sulfur cluster DNA helicase genetically linked to the chromosomal instability disorder Warsaw breakage syndrome. Tim stimulates DDX11 unwinding activity on forked DNA substrates up to 10-fold and on bimolecular anti-parallel G-quadruplex DNA structures and three-stranded D-loop approximately 4-5-fold. Electrophoretic mobility shift assays revealed that Tim enhances DDX11 binding to DNA, suggesting that the observed stimulation derives from an improved ability of DDX11 to interact with the nucleic acid substrate. Surface plasmon resonance measurements indicate that DDX11 directly interacts with Tim. DNA fiber track assays with HeLa cells exposed to hydroxyurea demonstrated that Tim or DDX11 depletion significantly reduced replication fork progression compared to control cells; whereas no additive effect was observed by co-depletion of both proteins. Moreover, Tim and DDX11 are epistatic in promoting efficient resumption of stalled DNA replication forks in hydroxyurea-treated cells. This is consistent with the finding that association of the two endogenous proteins in the cell extract chromatin fraction is considerably increased following hydroxyurea exposure. Overall, our studies provide evidence that Tim and DDX11 physically and functionally interact and act in concert to preserve replication fork progression in perturbed conditions. PMID:26503245

  17. [Heritability of body weight and fork length for Oncorhynchus masou masou].

    PubMed

    Zhang, Yu-Yong; Jia, Zhi-Ying; Bai, Qing-Li; Chen, Shu-Qiang; Shi, Lian-Yu; Wang, Bing-Qian

    2013-02-01

    Body weight and body length have been considered as the most important production traits for the fish genetic improvement. For cold-water fish, body length was usually substituted by fork length. In order to estimate the heritability of body weight and fork length of the sixth generation Oncorhynchus masou masou, which was introduced into China, the method of unbalanced nest design and an artificial insemination technigue were used. Twenty-nine full-sib families and fourteen half-sib families were obtained. Body weight and fork length of O. masou masou were measured in 12 and 24 months after fertilization. Based on full-sib and half-sib families data, the causal components of phenotypic variance were calculated. The results showed that, (1) during the whole growth phase of O. masou masou, the coefficient variation (CV) of fork length was higher than body weight, and CV of 12-month old was higher than that of 24-month old; (2) body weight and fork length of O. masou masou among sires and dams among sires were significant difference (P<0.01) both at 12 months and at 24 months; (3) the maternal component estimates were significantly larger than those of paternal ones for body weight and fork length traits both at 12 months and at 24 months; (4) for 12 months of O. masou masou the heritabilities of body weight and fork length were 0.41~0.51 and 0.46~0.54, respectively. For 24 months the values were 0.55~0.60 and 0.53~0.59, respectively; and (5) it was concluded that the heritability of growth traits in O. masou masou was relatively high and this highlights the potential to improve its growth through selective breeding. This study shows important data supporting for further genetic improvement of O. masou masou. PMID:23448933

  18. Geomorphic Effects of Engineered Log Jams in River Restoration, Middle Fork John Day River

    NASA Astrophysics Data System (ADS)

    Duffin, J.; McDowell, P. F.

    2014-12-01

    The Middle Fork of the John Day River (MFJD) Intensively Monitored Watershed in eastern Oregon is a multi-phase restoration implementation and monitoring project. MFJD is a tributary to the Colombia and is part of one of the longest free flowing rivers systems in the continental United States. It is a gravel and cobble bed river with a drainage area of 2,100 km2. The river has endured extensive channel and floodplain degradation from years of channel alteration and straightening due to human influences including dredge mining, ranching, and farming. As part of the river restoration project on the MFJD, engineered log jams have been constructed to address many of the restoration goals including creating scour pools, inhibiting bank erosion, creating and maintaining a sinuous river planform, and increasing complexity of fish habitat. There is a need for more detailed understanding on ELJ channel morphologic effects and how site-specific characteristics and differences in log jam infrastructure interact to create the in-channel features over timescales longer than a few years. This study uses detailed channel bed topographic surveys collected either with a total station or RTK-GPS technology. Geomorphic change detection techniques are utilized to monitor topographic change under and around the 26 log structures in two different river reaches over a six to seven year period The log structures are often associated with deepening of pools as desired, but also some structures show sedimentation under the structure. Differences in the patterns will be assessed based on the design, location, and specific characteristics of the log structures; variables include number and placement of logs, volume of structure, location on meander bend, and sediment sizes.

  19. Sources of alkalinity and acidity along an acid mine drainage remediated stream in SE Ohio: Hewett Fork

    NASA Astrophysics Data System (ADS)

    Schleich, K. L.; Lopez, D. A.; Bowman, J. R.; Kruse, N. A.; Mackey, A. L.; VanDervort, D.; Korenowsky, R.

    2013-12-01

    In the remediation of acid mine drainage impacted streams, it is important to locate and quantify the sources of acidity and alkalinity inputs. These parameters affect the long-term recovery of the stream habitat. Previous studies have focused on treating the remediation of AMD as point source pollution, targeting the main acid seep for remediation. However, in the interest of biological and chemical recovery, it is important to understand how sources of alkalinity and acidity, throughout the stream, affect water and sediment quality. The Hewett Fork watershed in Southeastern Ohio is impacted by AMD from the AS-14 mine complex in Carbondale, Ohio. In attempts to remediate the stream, the water is being treated with a continuous alkaline input from a calcium oxide doser. While the section of watershed furthest downstream from the doser is showing signs of recovery, the water chemistry and aquatic life near the doser are still impacted. The objective of this study is to examine and model the chemistry of the tributaries of Hewett Fork to see how they contribute to the alkalinity and acidity budgets of the main stem of the stream. By examining the inputs of tributaries into the main stem, this project aims to understand processes occurring during remediation throughout the entire stream. Discharge was measured during a dry period in October, 2012 and at a high flow in May, 2013. Field parameters such as pH, TDS, DO, alkalinity and acidity were also determined. Low flow data collected during fall sampling shows variable flow along the stream path, the stream gains water from ground water at some points while it loses water at others, potentially due to variable elevation of the water table. Flow data collected during spring sampling shows that Hewett Fork is a gaining stream during that period with inputs from groundwater contributing to increasing flow downstream. When using this data to calculate the net alkalinity load along the stream, there are areas with alkaline

  20. Historical perspectives on channel pattern in the Clark Fork River, Montana and implications for post-dam removal restoration

    NASA Astrophysics Data System (ADS)

    Woelfle-Erskine, C. A.; Wilcox, A. C.

    2009-12-01

    Active restoration approaches such as channel reconstruction have moved beyond the realm of small streams and are being applied to larger rivers. Uncertainties arising from limited knowledge, fluvial and ecosystem variability, and contaminants are especially significant in restoration of large rivers, where project costs and the social, infrastructural, and ecological costs of failure are high. We use the case of Milltown Dam removal on the Clark Fork River, Montana and subsequent channel reconstruction in the former reservoir to examine the use of historical research and uncertainty analysis in river restoration. At a cost of approximately $120 million, the Milltown Dam removal involves the mechanical removal of approximately 2 million cubic meters of sediments contaminated by upstream mining, followed by restoration of the former reservoir reach in which a single-thread meandering channel is being constructed. Historical maps, surveys, photographs, and accounts suggest a conceptual model of a multi-thread, anastomosing river in the reach targeted for channel reconstruction, upstream of the confluence of the Clark Fork and Blackfoot Rivers. We supplemented historical research with analysis of aerial photographs, topographic data, and USGS stage-discharge measurements in a lotic but reservoir-influenced reach of the Clark Fork River within our study area to estimate avulsion frequency (0.8 avulsions/year over a 70-year period) and average rates of lateral migration and aggradation. These were used to calculate the mobility number, a dimensionless relationship between channel filling and lateral migration timescales that can be used to predict whether a river’s planform is single or multi-threaded. The mobility number within our study reach ranged from 0.6 (multi-thread channel) to 1.7 (transitional channel). We predict that, in the absence of active channel reconstruction, the post-dam channel pattern would evolve to one that alternates between single and multi

  1. Mercury Contributions from Flint Creek and other Tributaries to the Upper Clark Fork River in Northwestern Montana

    NASA Astrophysics Data System (ADS)

    Langner, H.; Young, M.; Staats, M. F.

    2013-12-01

    Methylmercury contamination in biota is a major factor diminishing the environmental quality of the Upper Clark Fork River (CFR), e.g. by triggering human consumption limits of fish. The CFR is subject to one of the largest Superfund cleanup projects in the US, but remediation and restoration is currently focused exclusively on other mining-related contaminants (As, Cu, Zn, Pb, Cd), which may be counterproductive with respect to the bio-availability of mercury, for example by creation of wetlands along mercury-contaminated reaches of the river. The identification and elimination of Hg sources is an essential step toward reducing the methylmercury exposure in the biota of the CFR watershed because a strong correlation exists between total mercury levels in river sediment and methylmercury levels in aquatic life. We analyzed duplicate samples from the top sediment layer of the main stem and significant tributaries to the Clark Fork River along a 240 km reach between Butte, MT and downstream of the Missoula Valley. Mercury concentrations were 1.3 × 1.6 (mean × SD, n = 35) in the main stem. Concentrations in tributaries varied widely (0.02 to 85 mg/kg) and seemed only loosely related to the number of historic precious metal mines in the watershed. In the upper reach of the CFR, elevated Hg levels are likely caused by residual contaminated sediments in the flood plain. Levels tend to decrease downstream until Drummond, MT, where Flint Creek contributes a significant amount of mercury, causing Hg levels in the main stem CFR to increase from 0.7 to 4 mg/kg. Levels continue to decrease downstream. Flint Creek is the single largest contributor of Hg to the CFR. Detailed sampling of the main stem Flint Creek and tributaries (26 sites) showed extremely high levels in two tributaries (22 to 85 mg/kg) where historic milling operations were located. Elimination of these point sources may be accomplished comparatively economically and may significantly reduce mercury levels in

  2. Biological Monitoring Program for East Fork Poplar Creek

    SciTech Connect

    Adams, S.M.; Christensen, S.W.; Greeley, M.S.jr; Hill, W.R.; Kszos, L.A.; McCarthy, J.F.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.

    1998-10-15

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biologicai Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the compiex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC, These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumuiation studies, and (4) ecological surveys of the periphyton, benthic macro invertebrate, and fish communities. Monitoring is currently being conducted at five sites, although sites maybe excluded and/or others added depending upon the specific objectives of the various tasks. Criteria used in selecting the sites include: (1) location of sampling sites used in other studies, (2) known or suspected sources of downstream impacts, (3) proximity to U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR) boundaries, (4) concentration of mercury in the adjacent floodplain, (5) appropriate habitat distribution, and (6

  3. UvrD controls the access of recombination proteins to blocked replication forks.

    PubMed

    Lestini, Roxane; Michel, Bénédicte

    2007-08-22

    Blocked replication forks often need to be processed by recombination proteins prior to replication restart. In Escherichia coli, the UvrD repair helicase was recently shown to act at inactivated replication forks, where it counteracts a deleterious action of RecA. Using two mutants affected for different subunits of the polymerase III holoenzyme (Pol IIIh), we show here that the anti-RecA action of UvrD at blocked forks reflects two different activities of this enzyme. A defective UvrD mutant is able to antagonize RecA in cells affected for the Pol IIIh catalytic subunit DnaE. In this mutant, RecA action at blocked forks specifically requires the protein RarA (MgsA). We propose that UvrD prevents RecA binding, possibly by counteracting RarA. In contrast, at forks affected for the Pol IIIh clamp (DnaN), RarA is not required for RecA binding and the ATPase function of UvrD is essential to counteract RecA, supporting the idea that UvrD removes RecA from DNA. UvrD action on RecA is conserved in evolution as it can be performed in E. coli by the UvrD homologue from Bacillus subtilis, PcrA. PMID:17641684

  4. UvrD controls the access of recombination proteins to blocked replication forks

    PubMed Central

    Lestini, Roxane; Michel, Bénédicte

    2007-01-01

    Blocked replication forks often need to be processed by recombination proteins prior to replication restart. In Escherichia coli, the UvrD repair helicase was recently shown to act at inactivated replication forks, where it counteracts a deleterious action of RecA. Using two mutants affected for different subunits of the polymerase III holoenzyme (Pol IIIh), we show here that the anti-RecA action of UvrD at blocked forks reflects two different activities of this enzyme. A defective UvrD mutant is able to antagonize RecA in cells affected for the Pol IIIh catalytic subunit DnaE. In this mutant, RecA action at blocked forks specifically requires the protein RarA (MgsA). We propose that UvrD prevents RecA binding, possibly by counteracting RarA. In contrast, at forks affected for the Pol IIIh clamp (DnaN), RarA is not required for RecA binding and the ATPase function of UvrD is essential to counteract RecA, supporting the idea that UvrD removes RecA from DNA. UvrD action on RecA is conserved in evolution as it can be performed in E. coli by the UvrD homologue from Bacillus subtilis, PcrA. PMID:17641684

  5. Quartz tuning-fork oscillations in He II and drag coefficient

    NASA Astrophysics Data System (ADS)

    Gritsenko, I. A.; Zadorozhko, A. A.; Neoneta, A. S.; Chagovets, V. K.; Sheshin, G. A.

    2011-07-01

    The temperature dependencies of drag coefficient for quartz tuning forks of various geometric dimensions, immersed in the He II, were determined experimentally in the temperature range 0.1-3 K. It is identified, that these dependencies are similar, but the values of drag coefficient are different for tuning forks with different geometric dimensions. It is shown, that the obtained specific drag coefficient depends only on the temperature and frequency of vibrations, when the value of drag coefficient is normalized to the surface area of moving tuning-fork prong. The temperature dependencies of normalized drag coefficient for the tuning forks of various dimensions, wire, and microsphere, oscillating in the Не II, are compared. It is shown, that in the ballistic regime of scattering of quasiparticles, these dependencies are identical and have a slope proportional to T4, which is determined by the density of thermal excitations. In the hydrodynamic regime at T > 0.5 K, the behavior of the temperature dependence of specific drag coefficient is affected by the size and frequency of vibrating body. The empirical relation, which allows to describe the behavior of specific drag coefficient for vibrating tuning forks, microsphere, and wire everywhere over the temperature region and at various frequencies, is proposed.

  6. Tuning fork test utilization in detection of fractures: a review of the literature

    PubMed Central

    Kazemi, Mohsen

    1999-01-01

    A review of the literature was conducted to find relevant publications on the validity, reliability and utilization of the tuning fork test in detection of stress and simple fractures. Medline 1966-1998, Cumulative Index to Nursing and Allied Health Literature (CINAHL) 1982-1997, Science Citation Index 1961-1997, Index to chiropractic literature 1980-1998 and Chiropractic Research Archives Collection 1984-1990 data bases were searched. Key words such as tuning fork, vibration, diapason, fracture, stress fracture were used. The literature regarding the utilization of the tuning fork test in detection of fractures is very scarce. There was no study found in the above data bases on the validity and or reliability of the tuning fork test in detection of simple acute fractures. This review of the literature indicates the necessity of such a study since the tuning fork test has been used on the field for diagnosis of simple acute fractures. Imagesp124-ap124-bp124-cp124-dp124-e

  7. Vibratory Gyro-Sensor Using Vertically Set Quartz Crystal Trident-Type Tuning Fork Resonator

    NASA Astrophysics Data System (ADS)

    Shiratori, Norihiko; YoshiroTomikawa, YoshiroTomikawa; Ohnishi, Kazumasa

    1999-05-01

    In this study we deal with a new type of vibratory gyro-sensor using a vertically set quartz crystal trident-type tuning fork resonator. The sensor is made of X-cut quartz crystal wafer formed by rotating 2° about the X-axis, applying the wire saw cutting method. The slits that form three arms are cut along the Y-axis direction. In such a trident-type tuning fork resonator, two resonance vibration modes are used: one has vibrational displacement in the vertical direction (X-axis) of the tuning fork plane and the other has that in the horizontal direction (Z‧-axis). When an angular rate (ΩY‧) around the Y‧-axis is applied to the trident-type tuning fork gyro-sensor vibrating in the V-MODE, Coriolis forces, due to the ΩY‧, are applied on the three arms in the X‧-axis direction and the H-MODE vibration is induced. Therefore, the angular rate (ΩY‧) can be determined by detecting the signals of H-MODE vibration. The experimental results have proved that the vibratory gyro-sensor that uses such a quartz crystal tuning fork resonator has good characteristics.

  8. Regional Ecorisk Field investigation, upper Clark Fork River Basin

    SciTech Connect

    Pastorok, R.; LaTier, A.; Ginn, T.

    1995-12-31

    The Regional Ecorisk Field Investigation was conducted at the Clark Fork River Superfund Site (Montana) to evaluate the relationships between plant communities and tailings deposits in riparian habitats and to evaluate food-chain transfer of trace elements to selected wildlife species. Stations were selected to represent a range of vegetation biomass (or cover) values and apparent impact of trace elements, with some areas of lush vegetation, some areas of mostly unvegetated soil (e.g., < 30 percent plant cover), and a gradient in between. For the evaluation of risk to wildlife, bioaccumulation of metals was evaluated in native or naturalized plants, terrestrial invertebrates, and the deer mouse (Peromyscus maniculatus). Potential reproductive effects in the deer mouse were evaluated by direct measurements. For other wildlife species, bioaccumulation data were interpreted in the context of food web exposure models. Total biomass and species richness of riparian plant communities are related to tailings content of soil as indicated by pH and metals concentrations. Risk to populations of omnivorous small mammals such as the deer mouse was not significant. Relative abundance and reproductive condition of the deer mouse were normal, even in areas of high metals enrichment. Based on exposure models and site-specific tissue residue data for dietary species, risk to local populations of predators such as red fox and American kestrel that feed on deer mice and terrestrial invertebrates is not significant. Risk to herbivores related to metals bioaccumulation in plant tissues is not significant. Population level effects in deer and other large wildlife are not expected because of the large home ranges of such species and compensatory demographic factors.

  9. Two Dimensional Modeling of the Extent and Stability of Cold Water Refugia at the Confluences of the South Fork of the Eel River

    NASA Astrophysics Data System (ADS)

    Greer, G.; Thompson, S. E.

    2015-12-01

    Like many large rivers draining from forested uplands, the South Fork of the Eel River is characterized by a warm main stem fed by small cold water tributaries along its length. The confluences of the tributaries with the main stem form localized zones of cold water which provide habitat for anadromous fish species such as salmon. These cold water refugia are likely to be increasingly important for fish as temperatures warm and rainfall becomes more erratic due to projected climate change. These sites are protected from drying out over summer (unlike the tributaries, which can fragment if flows become too low), and they are accessible to migrating fish (as tributaries may not be if winter flows are too low to submerge in-channel obstacles). The overall significance of these refugia, however, is controlled by their spatial extent and temporal persistence. The factors that determine the shape, persistence, and stability of these refugia (as a function of varying flow and temperature regimes) remain poorly understood. A combination of scaling arguments and a 2D model is used to estimate the effects of varying flow and thermal regimes on the spatial extent of the cold water zones in the main stem. The stability of the cold water zones is also assessed in light of diurnal flow and temperature variations. The results are compared to in situ temperature data collected at the confluence of the South Fork of the Eel River and Elder Creek, and are used to project the likely significance of tributary confluences for generating cold-water habitat throughout the South Fork of the Eel River.

  10. WATER USE OF VITIS VINIFERA L., CV. THOMPSON SEEDLESS GROWN IN THE SAN JOAQUIN VALLEY OF CALIFORNIA. II. WATER USE OF MATURE VINES.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Water use of Thompson Seedless grapevines was measured with a large weighing lysimeter from four to seven years (1990 to 1993) after planting. Above ground drip irrigation was used to water the vines. Vines growing within the lysimeter were pruned to four and six fruiting canes for the 1990 and 19...

  11. "It Is Better to Light a Candle than to Curse the Darkness": Ethel Thompson Overby and Democratic Schooling in Richmond, Virginia, 1910-1958

    ERIC Educational Resources Information Center

    Ward Randolph, Adah L.

    2012-01-01

    In 1933, Ethel Thompson Overby became the first African American female principal in Richmond, Virginia. Her motto was "It is better to light a candle than to curse the darkness" (Overby 1975, 1). Before becoming principal, Overby had been a teacher in the southern urban "de jure" segregated schools of the city. How did the racially segregated…

  12. Carbon Dioxide, Hydrographic, and Chemical Data Obtained During the R/V Thomas G. Thompson Cruise in the Pacific Ocean

    SciTech Connect

    Sabine, C.L.; Key, R.M.; Hall, M.; Kozyr, A.

    1999-08-01

    This data documentation discusses the procedures and methods used to measure total carbon dioxide (TCO2), total alkalinity (TALK), and radiocarbon (delta 14C), at hydrographic stations, as well as the underway partial pressure of CO2 (pCO2) during the R/V Thomas G. Thompson oceanographic cruise in the Pacific Ocean (Section P10). Conducted as part of the World Ocean Circulation Experiment (WOCE), the cruise began in Suva, Fiji, on October 5, 1993, and ended in Yokohama, Japan, on November 10, 1993. Measurements made along WOCE Section P10 included pressure, temperature, salinity [measured by conductivity temperature, and depth sensor (CTD)], bottle salinity, bottle oxygen, phosphate, nitrate, silicate, chlorofluorocarbons (CFC-11, CFC-12), TCO2, TALK, delta 14C, and underway pCO2.

  13. Characteristics of Subcooled Liquid Methane During Passage Through a Spray-Bar Joule-Thompson Thermodynamic Vent System

    NASA Technical Reports Server (NTRS)

    Hastings, L. J.; Bolshinskiy, L. G.; Hedayat, A.; Schnell, A.

    2011-01-01

    NASA s Marshall Space Flight Center (MSFC) conducted liquid methane (LCH4) testing in November 2006 using the multipurpose hydrogen test bed (MHTB) outfitted with a spray-bar thermodynamic vent system (TVS). The basic objective was to identify any unusual or unique thermodynamic characteristics associated with subcooled LCH4 that should be considered in the design of space-based TVSs. Thirteen days of testing were performed with total tank heat loads ranging from 720 W to 420 W at a fill level of approximately 90%. During an updated evaluation of the data, it was noted that as the fluid passed through the Joule Thompson expansion, thermodynamic conditions consistent with the pervasive presence of metastability were indicated. This paper describes the observed thermodynamic conditions that correspond with metastability and effects on TVS performance.

  14. Linking habitat quality with trophic performance of steelhead along forest gradients in the South Fork Trinity River Watershed, California

    USGS Publications Warehouse

    McCarthy, Sarah G.; Duda, Jeffrey J.; Emlen, John M.; Hodgson, Garth R.; Beauchamp, David A.

    2009-01-01

    We examined invertebrate prey, fish diet, and energy assimilation in relation to habitat variation for steelhead Oncorhynchus mykiss (anadromous rainbow trout) and rainbow trout in nine low-order tributaries of the South Fork Trinity River, northern California. These streams spanned a range of environmental conditions, which allowed us to use bioenergetics modeling to determine the relative effects of forest cover, stream temperature, season, and fish age on food consumption and growth efficiency. Evidence of seasonal shifts in reliance on aquatic versus terrestrial food sources was detected among forest cover categories and fish ages, although these categories were not robust indicators of O. mykiss condition and growth efficiency. Consumption estimates were generally less than 20% of maximum consumption, and fish lost weight in some streams during summer low-flow conditions when stream temperatures exceeded 15°C. Current 100-year climate change projections for California threaten to exacerbate negative growth patterns and may undermine the productivity of this steelhead population, which is currently not listed as endangered or threatened. To demonstrate the potential effect of global warming on fish growth, we ran three climate change scenarios in two representative streams. Simulated temperature increases ranging from 1.4°C to 5.5°C during the summer and from 1.5°C to 2.9C during the winter amplified the weight loss; estimated average growth for age-1 fish was 0.4–4.5 times lower than normal (low to high estimated temperature increase) in the warm stream and 0.05–0.8 times lower in the cool stream. We conclude that feeding rate and temperature during the summer currently limit the growth and productivity of steelhead and rainbow trout in low-order streams in the South Fork Trinity River basin and predict that climate change will have detrimental effects on steelhead growth as well as on macroinvertebrate communities and stream ecosystems in general.

  15. The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants.

    PubMed

    Flores, Maria Jose; Bidnenko, Vladimir; Michel, Bénédicte

    2004-10-01

    Replication forks arrested by inactivation of the main Escherichia coli DNA polymerase (polymerase III) are reversed by the annealing of newly synthesized leading- and lagging-strand ends. Reversed forks are reset by the action of RecBC on the DNA double-strand end, and in the absence of RecBC chromosomes are linearized by the Holliday junction resolvase RuvABC. We report here that the UvrD helicase is essential for RuvABC-dependent chromosome linearization in E. coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli. PMID:15375374

  16. Pathological Changes of von Economo Neuron and Fork Neuron in Neuropsychiatric Diseases.

    PubMed

    Liu, Jia; Wang, Lu-ning; Arzberger, Thomas; Zhu, Ming-wei

    2016-02-01

    von Economo neuron (VEN) is a bipolar neuron characterized by a large spindle-shaped soma. VEN is generally distributed in the layer V of anterior insular lobe and anterior cingulate cortex. Fork neuron is another featured bipolar neuron. In recent years,many studies have illustrated that VEN and fork neurons are correlated with complicated cognition such as self-consciousness and social emotion. Studies in the development and morpholigies of these two neurons as well as their pathological changes in various neurological and psychiatric disorders have found that the abnormal number and functions of VEN can cause corresponding dysfunctions in social recognition and emotions both during the neuro-developmental stages of childhood and during the nerve degeneration in old age stage. Therefore, more attentions should be paid on the research of VEN and fork neurons in neuropsychiatric diseases. PMID:26996211

  17. The Coefficient of the Voltage Induced Frequency Shift Measurement on a Quartz Tuning Fork

    PubMed Central

    Hou, Yubin; Lu, Qingyou

    2014-01-01

    We have measured the coefficient of the voltage induced frequency shift (VIFS) of a 32.768 KHz quartz tuning fork. Three vibration modes were studied: one prong oscillating, two prongs oscillating in the same direction, and two prongs oscillating in opposite directions. They all showed a parabolic dependence of the eigen-frequency shift on the bias voltage applied across the fork, due to the voltage-induced internal stress, which varies as the fork oscillates. The average coefficient of the VIFS effect is as low as several hundred nano-Hz per millivolt, implying that fast-response voltage-controlled oscillators and phase-locked loops with nano-Hz resolution can be built. PMID:25414971

  18. Failure of origin activation in response to fork stalling leads to chromosomal instability at fragile sites.

    PubMed

    Ozeri-Galai, Efrat; Lebofsky, Ronald; Rahat, Ayelet; Bester, Assaf C; Bensimon, Aaron; Kerem, Batsheva

    2011-07-01

    Perturbed DNA replication in early stages of cancer development induces chromosomal instability preferentially at fragile sites. However, the molecular basis for this instability is unknown. Here, we show that even under normal growth conditions, replication fork progression along the fragile site, FRA16C, is slow and forks frequently stall at AT-rich sequences, leading to activation of additional origins to enable replication completion. Under mild replication stress, the frequency of stalling at AT-rich sequences is further increased. Strikingly, unlike in the entire genome, in the FRA16C region additional origins are not activated, suggesting that all potential origins are already activated under normal conditions. Thus, the basis for FRA16C fragility is replication fork stalling at AT-rich sequences and inability to activate additional origins under replication stress. Our results provide a mechanism explaining the replication stress sensitivity of fragile sites and thus, the basis for genomic instability during early stages of cancer development. PMID:21726815

  19. Ubiquitinated Fancd2 recruits Fan1 to stalled replication forks to prevent genome instability.

    PubMed

    Lachaud, Christophe; Moreno, Alberto; Marchesi, Francesco; Toth, Rachel; Blow, J Julian; Rouse, John

    2016-02-19

    Mono-ubiquitination of Fancd2 is essential for repairing DNA interstrand cross-links (ICLs), but the underlying mechanisms are unclear. The Fan1 nuclease, also required for ICL repair, is recruited to ICLs by ubiquitinated (Ub) Fancd2. This could in principle explain how Ub-Fancd2 promotes ICL repair, but we show that recruitment of Fan1 by Ub-Fancd2 is dispensable for ICL repair. Instead, Fan1 recruitment--and activity--restrains DNA replication fork progression and prevents chromosome abnormalities from occurring when DNA replication forks stall, even in the absence of ICLs. Accordingly, Fan1 nuclease-defective knockin mice are cancer-prone. Moreover, we show that a Fan1 variant in high-risk pancreatic cancers abolishes recruitment by Ub-Fancd2 and causes genetic instability without affecting ICL repair. Therefore, Fan1 recruitment enables processing of stalled forks that is essential for genome stability and health. PMID:26797144

  20. 77 FR 66865 - Record of Decision for the Oil and Gas Management Plan, Big South Fork National River and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... National Park Service Record of Decision for the Oil and Gas Management Plan, Big South Fork National River... (ROD) for the Oil and Gas Management Plan (Plan) for Big South Fork National River and Recreation Area... impacts from oil and gas development. The plan will guide oil and gas management in Big South...

  1. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  2. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  3. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  4. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  5. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  6. Geohydrologic appraisal of water resources of the South Fork, Long Island, New York

    USGS Publications Warehouse

    Nemickas, Bronius; Koszalka, Edward J.

    1982-01-01

    The ground-water resources of the South Fork of Long Island, N.Y., were investigated from April 1974 to September 1977. The study area encompasses 137 square miles and includes the eastern part of the Town of Southampton and the entire Town of East Hampton. The South Fork consists of a Paleozoic basement complex that is overlain by Cretaceous and Pleistocene sediments. The surficial material is composed of Late Wisconsinan glacial and glaciofluvial deposits in association with beach and marsh deposits of Recent age. Till underlies most of the eastern part of the South Fork. Precipitation is the sole source of fresh ground water on the South Fork. Average annual precipitation recorded at Bridgehampton from 1931-76 is 45 inches; about half this amount reaches the ground-water reservoir. It is estimated that overland runoff amounts to 0.5 inches per year, and evapotranspiration is 23 inches per year. Thus, recharge equals approximately 22 inches per year. Hydraulic conductivity and transmissivity of the Magothy (Cretaceous) and upper glacial (Pleistocene) aquifers on the South Fork were estimated from aquifer tests and specific-capacity data. The average horizontal hydraulic conductivity of the Magothy aquifer is 70 feet per day, and of the upper glacial aquifer 350 feet per day. Transmissivity of the Magothy aquifer on the South Fork ranges from 600 to 24,100 feet squared per day; transmissivity of the upper glacial aquifer ranges from 5,400 feet to 22,700 feet squared per day. No potable water is available from the underlying Lloyd aquifer. The position of the freshwater to saline-water interface is depicted in maps. In the southern part of the area, the freshwater reservoir follows the Ghyben-Herzberg principle, but in the northern part, the depth to interface is less than expected owing to a greater degree of anisotropy of the geologic units. Total public-supply pumpage on the South Fork is estimated to be about 3 Mgal/day, (million gallons per day). Public

  7. Measurements of Vortex Line Density Generated by a Quartz Tuning Fork in Superfluid 4He

    NASA Astrophysics Data System (ADS)

    Jackson, M. J.; Kolosov, O.; Schmoranzer, D.; Skrbek, L.; Tsepelin, V.; Woods, A. J.

    2016-05-01

    We present proof-of-concept measurements of the vortex line density generated by a quartz tuning fork resonator probed by the attenuation of second sound in superfluid ^4He at 1.6 K. The force-velocity response of a quartz tuning fork operating at a frequency of 31 kHz exhibited the onset of extra damping at a velocity of 0.5 ms^{-1}. Attenuation of the 5th resonant mode of second sound was observed at the same velocity, indicating the production of vortex lines. Our measurements demonstrate that an increase of the drag coefficient corresponds to the development of quantum turbulence.

  8. Real-Time Ozone Detection Based on a Microfabricated Quartz Crystal Tuning Fork Sensor

    PubMed Central

    Wang, Rui; Tsow, Francis; Zhang, Xuezhi; Peng, Jhih-Hong; Forzani, Erica S.; Chen, Yongsheng; Crittenden, John C.; Destaillats, Hugo; Tao, Nongjian

    2009-01-01

    A chemical sensor for ozone based on an array of microfabricated tuning forks is described. The tuning forks are highly sensitive and stable, with low power consumption and cost. The selective detection is based on the specific reaction of the polymer with ozone. With a mass detection limit of ∼2 pg/mm2 and response time of 1 second, the sensor coated with a polymer sensing material can detect ppb-level ozone in air. The sensor is integrated into a miniaturized wearable device containing a detection circuit, filtration, battery and wireless communication chip, which is ideal for personal and microenvironmental chemical exposure monitoring. PMID:22346720

  9. Calibrating the frequency of tuning forks by means of Lissajous figures

    NASA Astrophysics Data System (ADS)

    Quereda, Jorge; Ramón, Marina; Silva, Blanca; Hinarejos, Juan José; Rodrigo, José Gabriel; Farías, Daniel

    2011-05-01

    We produce Lissajous figures by modulating a laser beam along two perpendicular directions by means of two tuning forks. When the ratio of the resonant frequencies of the tuning forks is a small rational number, Lissajous figures are clearly resolved on a screen. Because the ratio of frequencies is never exactly a rational number, a phase drift of the Lissajous figures is observed as a function of time. If the period of this phase drift is measured, variations in the resonant frequencies as small as 0.01 Hz can be detected.

  10. Regulating telomere length from the inside out: the replication fork model

    PubMed Central

    2016-01-01

    Telomere length is regulated around an equilibrium set point. Telomeres shorten during replication and are lengthened by telomerase. Disruption of the length equilibrium leads to disease; thus, it is important to understand the mechanisms that regulate length at the molecular level. The prevailing protein-counting model for regulating telomerase access to elongate the telomere does not explain accumulating evidence of a role of DNA replication in telomere length regulation. Here I present an alternative model: the replication fork model that can explain how passage of a replication fork and regulation of origin firing affect telomere length. PMID:27401551

  11. Far field diffraction of an optical vortex beam by a fork-shaped grating

    NASA Astrophysics Data System (ADS)

    Stoyanov, Lyubomir; Topuzoski, Suzana; Stefanov, Ivan; Janicijevic, Ljiljana; Dreischuh, Alexander

    2015-09-01

    In this work we report experimental data confirming the analytically predicted transformation of the topological charge (TC) of an input optical vortex (OV) beam, generated by means of fork-shaped binary computer-generated hologram (CGH), after a second fork-shaped binary CGH. The final TC of the vortex is confirmed to be equal to the TC of the incident beam plus the diffraction order (with its sign) times the TC encoded in the binary grating. The radii of the transformed OVs in the far field also are found to agree fairly well with these predicted by the analytical theory.

  12. Delayed complete gastric outlet obstruction due to a dinner fork: report of a case.

    PubMed

    Atila, Koray; Unek, Tarkan; Sevinç, Ali Ibrahim; Aydoğan, Baki; Serin, Ayfer; Bora, Seymen; Gülay, Hüseyin

    2010-07-01

    Ingestion of foreign bodies can be a common problem especially among children, alcoholics, psychiatric patients, and senile patients, but ingestion of a metallic dinner fork is uncommon. Foreign bodies with smooth edges usually do not pose significant problems, but a sharp foreign object that is not retrieved at the earliest may penetrate the wall and cause complications. Ingested foreign bodies usually pass the intestinal tract without problems, and perforation occurs in less than 1%. In this paper, a case of rare gastric outlet obstruction due to a dinner fork, which was ingested 25 days before, is reported. PMID:20849060

  13. Fluvial sediment in Salem Fork watershed, West Virginia

    USGS Publications Warehouse

    Flint, R.F.

    1972-01-01

    Suspended sediment discharged from the 8.32-square-mile Salem Fork study area in Harrison County, W. Va., averaged 3,500 tons per year during the first 4-year period of investigation and 1,770 tons per year during the second 4-year period. The difference as attributed to increased flow control, effected by the completion of detention structures and other conservation measures, the absence of appreciable sediment-producing construction activities, and a reduction of the amounts of rainfall and runoff during the second 4-year period. Particle-size distribution of the suspended sediment discharged from the watershed remained unchanged during the two 4-year periods. Although sand and some silt were deposited in upstream reservoirs, sands and other sediments were evidently entrained in the flow below the reservoirs. During the 7.75-year period, reservoir 11A had a trap efficiency of 88 percent. The average annual sediment yield of subwatershed 11A was 1.31 tons per acre, or 837 tons per square mile. Outflow from reservoir 11A occurred during 81 percent of the investigation 'period, October 1954 to June 1962, and 78 percent of the sediment discharge from the reservoir occurred during less than 6 percent of the investigation period. A comparison of particle-size distribution of inflow sediment with that of outflow sediment revealed that practically all sands and some silts entering reservoir 11A were deposited in the reservoir. Chemical analyses of inflow water and the particle-size analyses suggested that flocculation of fine sediments occurred in the reservoir. Analysis of the sediment data collected at the outflow of reservoir 9 during 1956-62 revealed that the average annual sediment discharge was 128,000 pounds per year. Limited particle-size data suggested that practically no sand was discharged from reservoir 9, even though the inflow contained sand. Average annual inflow to reservoirs 11A and 9 compared favorably with average annual runoff for the entire watershed

  14. Time of travel of solutes in the East Fork Trinity River, November 1975; and Elm Fork Trinity River, December 1975; Trinity River Basin, Texas

    USGS Publications Warehouse

    Myers, Dennis R.; Slade, Raymond M., Jr.

    1976-01-01

    The U.S. Geological Survey, in cooperation with the North Central Texas Council of Governments, the Trinity River Authority of Texas, and the Texas Water Development Board, conducted two time-of-travel studies in the Trinity River basin in November and December, 1975.  Field data were collected on the East Fork Trinity River during November 18-22, 1975, and on the Elm Fork Trinity River during December 8-13, 1975.  The purpose of these two studies was to provide data that could be used by the Trinity River Authority and the Texas Water Quality Board in the development of a mathematical water-quality model of the two streams.  The model is to be used in a comprehensive water-quality management plan for the Trinity River basin.

  15. EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair

    PubMed Central

    Wu, Yuehan; Lee, Suk-Hee; Williamson, Elizabeth A.; Reinert, Brian L.; Cho, Ju Hwan; Xia, Fen; Jaiswal, Aruna Shanker; Srinivasan, Gayathri; Patel, Bhavita; Brantley, Alexis; Zhou, Daohong; Shao, Lijian; Pathak, Rupak; Hauer-Jensen, Martin; Singh, Sudha; Kong, Kimi; Wu, Xaiohua; Kim, Hyun-Suk; Beissbarth, Timothy; Gaedcke, Jochen; Burma, Sandeep; Nickoloff, Jac A.; Hromas, Robert A.

    2015-01-01

    Replication fork stalling and collapse is a major source of genome instability leading to neoplastic transformation or cell death. Such stressed replication forks can be conservatively repaired and restarted using homologous recombination (HR) or non-conservatively repaired using micro-homology mediated end joining (MMEJ). HR repair of stressed forks is initiated by 5’ end resection near the fork junction, which permits 3’ single strand invasion of a homologous template for fork restart. This 5’ end resection also prevents classical non-homologous end-joining (cNHEJ), a competing pathway for DNA double-strand break (DSB) repair. Unopposed NHEJ can cause genome instability during replication stress by abnormally fusing free double strand ends that occur as unstable replication fork repair intermediates. We show here that the previously uncharacterized Exonuclease/Endonuclease/Phosphatase Domain-1 (EEPD1) protein is required for initiating repair and restart of stalled forks. EEPD1 is recruited to stalled forks, enhances 5’ DNA end resection, and promotes restart of stalled forks. Interestingly, EEPD1 directs DSB repair away from cNHEJ, and also away from MMEJ, which requires limited end resection for initiation. EEPD1 is also required for proper ATR and CHK1 phosphorylation, and formation of gamma-H2AX, RAD51 and phospho-RPA32 foci. Consistent with a direct role in stalled replication fork cleavage, EEPD1 is a 5’ overhang nuclease in an obligate complex with the end resection nuclease Exo1 and BLM. EEPD1 depletion causes nuclear and cytogenetic defects, which are made worse by replication stress. Depleting 53BP1, which slows cNHEJ, fully rescues the nuclear and cytogenetic abnormalities seen with EEPD1 depletion. These data demonstrate that genome stability during replication stress is maintained by EEPD1, which initiates HR and inhibits cNHEJ and MMEJ. PMID:26684013

  16. Recent sedimentation and surface-water flow patterns on the flood plain of the North Fork Forked Deer River, Dyer County, Tennessee

    USGS Publications Warehouse

    Wolfe, W.J.; Diehl, T.H.

    1993-01-01

    Sedimentation in the 19th and 20th centuries has had a major effect on surface-water drainage conditions along a 7-mile section of the North, Fork Forked Deer River flood plain, Dyer County, Tenn. During the century prior to 1930, 5 to 12 feet of sediment were deposited over much of the flood plain, resulting in channel obstruction and widespread flooding. The estimated bankfull capacity of the natural channel before it was channelized in 19 16 was comparable to the base flow of the river during the 1980's. Ditching of the river between 191i6 and 1;9,21 was followed by reductions in sedimentation rates over parts of the flood plain. However, the effects of sedimentation have persisted. Occlusions along the natural channel of the river have divided this stream reach into a series of sloughs. These sloughs continue to fill with sediment and are surrounded by ponds that have expanded since 1941. Degradation of the North Fork Forked Deer ditch may eventually reduce ponding over much of the flood plain. Active incision of headcuts in both banks of the ditch is enhancing the drainage of widespread ponded areas. These headcuts likely will have limited effect on drainage of most tributaries. The highest recent sedimentation rates, in places more than 0.2 foot per year, are concentrated near the flood-plain margin along tributary streams. In conjunction with beaver dams and debris, ongoing sedimentation has blocked flow in several tributaries, posing a flood hazard to agricultural land near the flood-plain margin. The occluded tributaries likely will continue to overflow unless they are periodically dredged or their sediment loads are reduced.

  17. Channelization and floodplain forests: Impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

    USGS Publications Warehouse

    Oswalt, S.N.; King, S.L.

    2005-01-01

    We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased overbank flooding, a rise in the groundwater table, and ponding of upstream timber. Our objectives were to: (1) determine the composition of floodplain vegetation communities along the degraded river reach, (2) to isolate relationships among these communities, geomorphic features, and environmental variables and (3) evaluate successional changes based on current stand conditions. Vegetation communities were not specifically associated with predefined geomorphic features; nevertheless, hydrologic and geomorphic processes as a result of channelization have clearly affected vegetation communities. The presence of valley plugs and continued degradation of upstream reaches and tributaries on the impacted study reach has arrested recovery of floodplain plant communities. Historically common species like Liquidambar styraciflua L. and Quercus spp. L. were not important, with importance values (IV) less than 1, and occurred in less than 20% of forested plots, while Acer rubrum L., a disturbance-tolerant species, was the most important species on the site (IV = 78.1) and occurred in 87% of forested plots. The results of this study also indicate that channelization impacts on the Middle Fork Forked Deer River are more temporally and spatially complex than previously described for other river systems. Rehabilitation of this system necessitates a long-term, landscape-scale solution that addresses watershed rehabilitation in a spatially and temporally hierarchical manner. ?? 2005 Elsevier B.V. All rights reserved.

  18. Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

    SciTech Connect

    Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; Juhasz, Szilvia; Morocz, Monika; Gali, Himabindu; Dhe-Paganon, Sirano; Venclovas, Česlovas; Haracska, Lajos

    2015-09-08

    Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteins retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. We suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal.

  19. 77 FR 71611 - Land Acquisitions; North Fork Rancheria of Mono Indians of California

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-03

    ... Tribe's current government headquarters, which are located in the town of North Fork, Madera County, California, described as: Real property in the City of UNINCORPORATED AREA, County of Madera, State of..., AND 8 of PARCEL MAP 3426 IN THE UNINCORPORATED AREA OF THE COUNTY OF MADERA, STATE OF CALIFORNIA,...

  20. Inferring Causes of Biological Impairment in the Clear Fork Watershed, West Virginia (Final)

    EPA Science Inventory

    The Clear Fork, WV case study illustrates a causal assessment in a watershed using the USEPA stressor identification process described on www.epa.gov/caddis. This case study demonstrates that a watershed-wide causal assessment has several advantages for making analysis practical,...

  1. Double Moutain Fork Brazos River between Lubbock and Buffalo Lakes, Texas

    USGS Publications Warehouse

    Joerns, J.O.

    1955-01-01

    The purpose of this investigation was to study quantity, quality, and possible sources of the low flow and spring inflow of the Double Mountain Fork of the Brazos River between the Lubbock sewage disposal plant, 3 miles southeast of Lubbock, and a County Road crossing about 15 miles downstream and 4.2 miles northeast of Slaton, Lubbock County (fig. 1).

  2. 77 FR 23059 - Endangered and Threatened Wildlife and Plants; Determination of Endangered Status for Three Forks...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-17

    ... Forks springsnail as a candidate for listing on October 30, 2001 (66 FR 54808). We first identified the San Bernardino springsnail as a candidate for listing on December 6, 2007 (72 FR 69034). Candidates... Bernardino springsnail. In our most recent annual Candidate Notice of Review dated November 10, 2010 (75...

  3. Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling.

    PubMed

    Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; Juhasz, Szilvia; Morocz, Monika; Gali, Himabindu; Dhe-Paganon, Sirano; Venclovas, Česlovas; Haracska, Lajos

    2015-12-01

    Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteins retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. In more general terms, we suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal. PMID:26350214

  4. Assessment of the Iowa River's South Fork Watershed: Part 2. Conservation Practices

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Assessments of conservation practice effects on water quality in watersheds require the types and extent of practices implemented to be known. This paper assesses results from a conservation-practices inventory for the South Fork of the Iowa River (78,000 ha), conducted in 2005. Agricultural managem...

  5. "Forked Tongue: The Politics of Bilingual Education," by Rosalie Pedalino Porter. Book Review.

    ERIC Educational Resources Information Center

    Amabisca, Anastasia Aimee

    1994-01-01

    Based on personal experience rather than empirical evidence, "Forked Tongue" presents a biased view that could damage the future of bilingual education in the United States. The book considers bilingual education a threat because it prolongs the learning of English and promotes the maintenance of other "un-American" languages that prevent…

  6. Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

    DOE PAGESBeta

    Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; Juhasz, Szilvia; Morocz, Monika; Gali, Himabindu; Dhe-Paganon, Sirano; Venclovas, Česlovas; Haracska, Lajos

    2015-09-08

    Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteinsmore » retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. We suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal.« less

  7. CLARK FORK RIVER AND LAKE PEND OREILLE - IDHW-DOE WATER QUALITY STUDY, 1984

    EPA Science Inventory

    Under a cooperative agreement, U.S. Geological Survey technicians have been measuring river flow and suspended sediment loads and collecting water samples for laboratory analysis on a monthly basis since July 1984 for the Clark Fork River and Lake Pend Oreille, Idaho (17010213). ...

  8. SOUTH FORK COEUR D'ALENE RIVER, NORTHERN IDAHO. DISTRIBUTION OF HEAVY METAL LOADINGS

    EPA Science Inventory

    The purpose of this study is to determine the current distribution of metals loadings to the South Fork Coeur dAlene River, Idaho (17010301, 17010303). Water quality and flow data obtained from EPA Region 10 for September 1986 and September 1987 are used to determine loadings du...

  9. Do sister forks of bidirectionally growing replicons proceed at unequal rates

    SciTech Connect

    Dubey, D.D.; Raman, R.

    1987-02-01

    DNA fibre autoradiography in different tissues of the rodents Bandicota bengalensis and Nesokia indica reveals a high frequency of such bidirectionally replicating replicons whose sister hot tracks are of unequal size. These results suggest intrarepliconic difference in the rates of fork migration in the two directions.

  10. A soil quality assessment within the Iowa River South Fork Watershed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Soil quality assessment is a proactive process for understanding the long-term effects of crop and soil management practices within agricultural watersheds. Fields with both well-developed and poor (N-deficient) corn (Zea mays L.) canopy growth were identified within the Iowa River’s South Fork Wate...

  11. HERC2 Interacts with Claspin and regulates DNA origin firing and replication fork progression.

    PubMed

    Izawa, Naoki; Wu, Wenwen; Sato, Ko; Nishikawa, Hiroyuki; Kato, Akihiro; Boku, Narikazu; Itoh, Fumio; Ohta, Tomohiko

    2011-09-01

    DNA replication, recombination, and repair are highly interconnected processes the disruption of which must be coordinated in cancer. HERC2, a large HECT protein required for homologous recombination repair, is an E3 ubiquitin ligase that targets breast cancer suppressor BRCA1 for degradation. Here, we show that HERC2 is a component of the DNA replication fork complex that plays a critical role in DNA elongation and origin firing. In the presence of BRCA1, endogenous HERC2 interacts with Claspin, a protein essential for G(2)-M checkpoint activation and replication fork stability. Claspin depletion slowed S-phase progression and additional HERC2 depletion reduced the effect of Claspin depletion. In addition, HERC2 interacts with replication fork complex proteins. Depletion of HERC2 alleviated the slow replication fork progression in Claspin-deficient cells, suppressed enhanced origin firing, and led to a decrease in MCM2 phosphorylation. In a HERC2-dependent manner, treatment of cells with replication inhibitor aphidicolin enhanced MCM2 phosphorylation. Taken together, our results suggest that HERC2 regulates DNA replication progression and origin firing by facilitating MCM2 phosphorylation. These findings establish HERC2 as a critical function in DNA repair, checkpoint activation, and DNA replication. PMID:21775519

  12. NUTRIENT CONCENTRATIONS IN FLOWING WATERS OF THE SOUTH FORK BROAD RIVER, GEORGIA WATERSHED

    EPA Science Inventory

    We monitored concentrations of nutrients, dissolved organic matter (DOM) and other parameters in 17 headwater streams, at three sites on the main stem, and in three major tributaries near their confluence with the South Fork Broad River on a monthly basis for over a year. Concent...

  13. EFFECTS OF HABITAT DEGRADATION ON BIOLOGICAL ENDPOINTS IN THE SOUTH FORK BROAD RIVER BASIN, GEORGIA

    EPA Science Inventory

    Many of the streams of the lower Piedmont ecoregion in Georgia have been negatively impacted to some degree by habitat degradation due primarily to sedimentation. The South Fork of the Broad River watershed has been designated as sediment impacted under Section 303(d) of the Clea...

  14. BENZO(A)PYRENE DIOL EPOXIDE I BINDS TO DNA AT REPLICATION FORKS (JOURNAL VERSION)

    EPA Science Inventory

    The distribution in replication forks of DNA lesions caused by the treatment of S phase calls with benzo(a)pyrene-diol-epoxide-1 (BPDE-1) was studied in synchronized C3H10T1/2 cells. Sites of carcinogen modification of DNA were identified by polyclonal rabbit antibodies that were...

  15. Conservation Practices and Water Quality in the Iowa River's South Fork Watershed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Iowa River’s South Fork drains 87,000 ha under intensive, high-production agriculture. Corn-soybean rotations occupy about 85% of the watershed. About 100 swine CAFOs generate manure applied onto about a quarter of the watershed annually. Artificial drainage is extensive because the young glacia...

  16. Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

    PubMed Central

    Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; Juhasz, Szilvia; Morocz, Monika; Gali, Himabindu; Dhe-Paganon, Sirano; Venclovas, Česlovas; Haracska, Lajos

    2015-01-01

    Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteins retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. In more general terms, we suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal. PMID:26350214

  17. Assessment of the Iowa River's South Fork Watershed: Part 1. Water Quality

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The South Fork of the Iowa River drains 78,000 ha under intensive, high-production agriculture. About 100 swine CAFOs generate manure applied onto about a quarter of the watershed annually. Hydric soils cover 54% of the watershed because the young glacial terrain is poorly dissected. Artificial subs...

  18. KOOTENAI, CLARK FORK, PEND OREILLE, AND SPOKANE RIVER BASINS, WATER QUALITY ASSESSMENT, 1976

    EPA Science Inventory

    This report contains a water quality assessment approach which will assist EPA planners, land agencies, and state and local agencies in identifying probably nonpoint sources and determining their effects upon the fishable-swimmable aspect of the Kootenai, Clark Fork-Pend Oreille,...

  19. LIFE HISTORY MONITORING OF SALMONIDS IN THE WEST FORK SMITH RIVER, UMPQUA BASIN, OREGON

    EPA Science Inventory

    As a life-cycle monitoring basin for the Oregon Salmon Plan, the Oregon Department of Fish and Wildlife has estimated adult returns, distribution and smolt outmigration of coho, chinook and winter steelhead in the West Fork Smith River since 1998. In 2001/2002, the Environmenta...

  20. ESCHERICHIA COLI AND ENTEROCOCCUS IN THE SOUTH FORK OF THE IOWA RIVER WATERSHED

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Confined swine production has increased in Iowa over the last decade and there is uncertainty about the impact of these operations on water quality. We have monitored the populations of Escherichia coli and Enterococcus in the 78,000 ha South Fork of the Iowa River watershed from 2003 through 2005 t...

  1. NUTRIENT CONCENTRATIONS IN FLOWING WATERS OF THE SOUTH FORK BROAD RIVER, GEORGIA WATERSHED

    EPA Science Inventory

    The South Fork Broad River (SFBR) drains about 635 km2 of the Georgia Piedmont. The SFBR watershed is primarily rural and undeveloped although the human population increased by about 25% between 1990 and 2000. Forestry and agriculture are the main land uses. Agriculture consis...

  2. Structure of the replication fork in ultraviolet light-irradiated human cells.

    PubMed Central

    Cordeiro-Stone, M; Schumacher, R I; Meneghini, R

    1979-01-01

    The DNA extracted from xeroderma pigmentosum human fibroblasts previously irradiated with 12.5 J/m2 of UV light and pulse-labeled for 45 min with radioactive and (or) heavy precursors, was used to determine the structural characteristics of the replication fork. Density equilibrium centrifugation experiments showed that a fork moved 6 micrometer in 45 min and bypassed 3 pyrimidine dimers in both strands. The same length was covered in 15-20 min in control cells. The delay in irradiated cells was apparently due to pyrimidine dimers acting as temporary blocks to the fork movement. Evidence for this interpretation comes from kinetics of incorporation of [3H]thymidine into DNA, which show that the time necessary to attain a new stable level of DNA synthesis in irradiated cells is equivalent to that required for the replication fork to cover the interdimer distance in one strand. On the other hand, the action of S1 nuclease on DNA synthesized soon after irradiation gives rise to a bimodal distribution in neutral sucrose gradients, one peak corresponding to 43 X 10(6) daltons and the other to 3 X 10(6) daltons. These two DNA species are generated by the attack of the S1 nuclease on single-stranded regions associated with the replication fork. A possible explanation for these results is given by a model according to which there is a delayed bypass of the dimer in the leading strand and the appearance of gaps opposite pyrimidine dimers in the lagging strand, as a direct consequence of the discontinuous mode of DNA replication. In terms of the model, the DNA of 43 X 10(6) daltons corresponds to the leading strand, linked to the unreplicated branch of the forks, whereas the piece of 3 X 10(6) daltons is the intergap DNA coming from the lagging strand. Pulse and chase experiments reveal that the low molecular weight DNA grows in a pattern that suggests that more than one gap may be formed per replication fork. PMID:233582

  3. Studies of kinetic processes in a concentrated 3He-4He solution using an oscillating tuning fork

    NASA Astrophysics Data System (ADS)

    Bakhvalova, V. A.; Gritsenko, I. A.; Rudavskii, E. Ya.; Chagovets, V. K.; Sheshin, G. A.

    2015-07-01

    The dissipative processes causing the damping of quartz tuning fork vibrations in a solution of 15% 3He in 4He, are studied in a temperature range of 0.5-2.3 K. The resonance curves of the tuning forks are measured in the laminar flow region of the liquid, and their width is determined by the width of the dissipative processes. We examined tuning forks with a resonance frequency of 32 kHz, located inside a flask ("enclosed") and tuning forks without a flask ("unenclosed"). The results of the experiment are compared to existing theories. It was found that a significant contribution to the damping of tuning fork oscillations for a solution, as opposed to pure 4He, is from the second sound radiation, the contribution of which exceeds the input of viscous dissipation at low temperatures. The radiation of the first sound does not contribute to the damping of the oscillations of the "enclosed" fork due to the small size of the cell versus the wavelength. In the case of the "unenclosed" fork, the damping is determined by three processes: viscous dissipation and radiation of the first and second sounds.

  4. Effects of rainbow trout fry of a metals-contaminated diet of benthic invertebrates from the Clark Fork River, Montana

    SciTech Connect

    Woodward, D.F. ); Brumbaugh, W.G.; DeLonay, A.J.; Little, E.E. ); Smith, C.E. )

    1994-01-01

    The upper Clark Fork River in northwestern Montana has received mining wastes from the Butte and Anaconda areas since 1880. These wastes have contaminated areas of the river bed and floodplain with tailings and heavy metal sludge, resulting in elevated concentration of metals in surface water, sediments, and biota. Rainbow trout Oncorhynchus mykiss were exposed immediately after hatching for 91 d to cadmium, copper, lead, and zinc in water at concentrations simulating those in Clark Fork River. From exogenous feeding (21 d posthatch) through 91 d, fry were also fed benthic invertebrates from the Clark Fork River that contained elevated concentrations of arsenic, cadmium, copper, and lead. Evaluations of different combinations of diet and water exposure indicated diet-borne metals were more important than water-borne metals - at the concentrations we tested - in reducing survival and growth of rainbow trout. Whole-body metal concentrations ([mu]g/g, wet weight) at 91 d in fish fed Clark Fork invertebrates without exposure to Clark Fork water were arsenic, 1.4; cadmium, 0.16; and copper, 6.7. These were similar to concentrations found in Clark Fork River fishes. Livers from fish on the high-metals diets exhibited degenerative changes and generally lacked glycogen vacuolation. Indigenous Clark Fork River invertebrates provide a concentrated source of metals for accumulation into young fishes, and probably were the cause of decreased survival and growth of age-0 rainbow trout in our laboratory exposures. 30 refs., 8 figs., 4 tabs.

  5. Dissolved-Solids Load in Henrys Fork Upstream from the Confluence with Antelope Wash, Wyoming, Water Years 1970-2009

    USGS Publications Warehouse

    Foster, Katharine; Kenney, Terry A.

    2010-01-01

    Annual dissolved-solids load at the mouth of Henrys Fork was estimated by using data from U.S. Geological Survey streamflow-gaging station 09229500, Henrys Fork near Manila, Utah. The annual dissolved-solids load for water years 1970-2009 ranged from 18,300 tons in 1977 to 123,300 tons in 1983. Annual streamflows for this period ranged from 14,100 acre-feet in 1977 to 197,500 acre-feet in 1983. The 25-percent trimmed mean dissolved-solids load for water years 1970-2009 was 44,300 tons per year at Henrys Fork near Manila, Utah. Previous simulations using a SPAtially Referenced Regression On Watershed attributes (SPARROW) model for dissolved solids specific to water year 1991 conditions in the Upper Colorado River Basin predicted an annual dissolved-solids load of 25,000 tons for the Henrys Fork Basin upstream from Antelope Wash. On the basis of computed dissolved-solids load data from Henrys Fork near Manila, Utah, together with estimated annual dissolved-solids load from Antelope Wash and Peoples Canal, this prediction was adjusted to 37,200 tons. As determined by simulations with the Upper Colorado River Basin SPARROW model, approximately 56 percent (14,000 tons per year) of the dissolved-solids load at Henrys Fork upstream from Antelope Wash is associated with the 21,500 acres of irrigated agricultural lands in the upper Henrys Fork Basin.

  6. Direct comparisons of hand and mouth kinematics during grasping, feeding and fork-feeding actions

    PubMed Central

    Quinlan, D. J.; Culham, J. C.

    2015-01-01

    While a plethora of studies have examined the kinematics of human reach-to-grasp actions, few have investigated feeding, another ethologically important real-world action. Two seminal studies concluded that the kinematics of the mouth during feeding are comparable to those of the hand during grasping (Castiello, 1997; Churchill et al., 1999); however, feeding was done with a fork or spoon, not with the hand itself. Here, we directly compared grasping and feeding kinematics under equivalent conditions. Participants were presented with differently sized cubes of cheese (10-, 20- or 30-mm on each side) and asked to use the hand to grasp them or to use a fork to spear them and then bring them to the mouth to bite. We measured the apertures of the hand during grasping and the teeth during feeding, as well as reaching kinematics of the arm in both tasks. As in many past studies, we found that the hand oversized considerably larger (~11–27 mm) than the food item during grasping; moreover, the amount of oversizing scaled with food size. Surprisingly, regardless of whether the hand or fork was used to transport the food, the mouth oversized only slightly larger (~4–11 mm) than the food item during biting and the oversizing did not increase with food size. Total movement times were longer when using the fork compared to the hand, particularly when using the fork to bring food to the mouth. While reach velocity always peaked approximately halfway through the movement, relative to the reach the mouth opened more slowly than the hand, perhaps because less time was required for the smaller oversizing. Taken together, our results show that while many aspects of kinematics share some similarity between grasping and feeding, oversizing may reflect strategies unique to the hand vs. mouth (such as the need to have the digits approach the target surface perpendicularly for grip stability during lifting) and differences in the neural substrates of grasping and feeding. PMID

  7. Groundwater and climate change: a sensitivity analysis for the Grand Forks aquifer, southern British Columbia, Canada

    NASA Astrophysics Data System (ADS)

    Allen, D. M.; Mackie, D. C.; Wei, M.

    The Grand Forks aquifer, located in south-central British Columbia, Canada was used as a case study area for modeling the sensitivity of an aquifer to changes in recharge and river stage consistent with projected climate-change scenarios for the region. Results suggest that variations in recharge to the aquifer under the different climate-change scenarios, modeled under steady-state conditions, have a much smaller impact on the groundwater system than changes in river-stage elevation of the Kettle and Granby Rivers, which flow through the valley. All simulations showed relatively small changes in the overall configuration of the water table and general direction of groundwater flow. High-recharge and low-recharge simulations resulted in approximately a +0.05 m increase and a -0.025 m decrease, respectively, in water-table elevations throughout the aquifer. Simulated changes in river-stage elevation, to reflect higher-than-peak-flow levels (by 20 and 50%), resulted in average changes in the water-table elevation of 2.72 and 3.45 m, respectively. Simulated changes in river-stage elevation, to reflect lower-than-baseflow levels (by 20 and 50%), resulted in average changes in the water-table elevation of -0.48 and -2.10 m, respectively. Current observed water-table elevations in the valley are consistent with an average river-stage elevation (between current baseflow and peak-flow stages). L'aquifère de Grand Forks, situé en Colombie britannique (Canada), a été utilisé comme zone d'étude pour modéliser la sensibilité d'un aquifère à des modifications de la recharge et du niveau de la rivière, correspondant à des scénarios envisagés de changement climatique dans cette région. Les résultats font apparaître que les variations de recharge de l'aquifère pour différents scénarios de changement climatique, modélisées pour des conditions de régime permanent, ont un impact sur le système aquifère beaucoup plus faible que les changements du niveau des

  8. Groundwater and climate change: a sensitivity analysis for the Grand Forks aquifer, southern British Columbia, Canada

    NASA Astrophysics Data System (ADS)

    Allen, D. M.; Mackie, D. C.; Wei, M.

    The Grand Forks aquifer, located in south-central British Columbia, Canada was used as a case study area for modeling the sensitivity of an aquifer to changes in recharge and river stage consistent with projected climate-change scenarios for the region. Results suggest that variations in recharge to the aquifer under the different climate-change scenarios, modeled under steady-state conditions, have a much smaller impact on the groundwater system than changes in river-stage elevation of the Kettle and Granby Rivers, which flow through the valley. All simulations showed relatively small changes in the overall configuration of the water table and general direction of groundwater flow. High-recharge and low-recharge simulations resulted in approximately a +0.05 m increase and a -0.025 m decrease, respectively, in water-table elevations throughout the aquifer. Simulated changes in river-stage elevation, to reflect higher-than-peak-flow levels (by 20 and 50%), resulted in average changes in the water-table elevation of 2.72 and 3.45 m, respectively. Simulated changes in river-stage elevation, to reflect lower-than-baseflow levels (by 20 and 50%), resulted in average changes in the water-table elevation of -0.48 and -2.10 m, respectively. Current observed water-table elevations in the valley are consistent with an average river-stage elevation (between current baseflow and peak-flow stages). L'aquifère de Grand Forks, situé en Colombie britannique (Canada), a été utilisé comme zone d'étude pour modéliser la sensibilité d'un aquifère à des modifications de la recharge et du niveau de la rivière, correspondant à des scénarios envisagés de changement climatique dans cette région. Les résultats font apparaître que les variations de recharge de l'aquifère pour différents scénarios de changement climatique, modélisées pour des conditions de régime permanent, ont un impact sur le système aquifère beaucoup plus faible que les changements du niveau des

  9. Drilling, construction, and aquifer-test data from wells 3-3307-20 and -21, Thompson Corner exploratory wells I and II, Oahu, Hawaii

    USGS Publications Warehouse

    Presley, T.K.; Oki, D.S.

    1996-01-01

    The Thompson Corner exploratory wells I and II (State well numbers 3-3307-20 and -21) were drilled near Thompson Corner, about 2.2 miles south-southwest of the town of Haleiwa. The wells are located on agricultural land in the Waialua ground-water area. The wells are about 50 feet apart and penetrate about 90 feet into the ground water. Aquifer tests were conducted using well 3-3307-20 as a pumping well and well 3-3307-21 as an observation well. Well-construction data, logs of drilling notes, geologic descriptions for the samples, and aquifer-test data are presented for the wells. The wells are two of twelve exploratory wells drilled in the north-central Oahu area between July 1993 and May 1994 in cooperation with the Honolulu Board of Water Supply.

  10. A one-dimensional, steady-state, dissolved-oxygen model and waste-load assimilation study for West Fork Blue River, Washington County, Indiana

    USGS Publications Warehouse

    Peters, James G.; Wilber, W.G.; Crawford, Charles G.; Girardi, F.P.

    1979-01-01

    A digital computer model calibrated to observe stream conditions was used to evaluate water quality in West Fork Blue River, Washington County, IN. Instream dissolved-oxygen concentration averaged 96.5% of saturation at selected sites on West Fork Blue River during two 24-hour summer surveys. This high dissolved-oxygen concentration reflects small carbonaceous and nitrogenous waste loads; adequate dilution of waste by the stream; and natural reaeration. Nonpoint source waste loads accounted for an average of 53.2% of the total carbonaceous biochemical-oxygen demand and 90.2% of the nitrogenous biochemical-oxygen demand. Waste-load assimilation was studiedfor critical summer and winter low flows. Natural streamflow for these conditions was zero, so no benefit from dilution was provided. The projected stream reaeration capacity was not sufficient to maintain the minimum daily dissolved-oxygen concentration (5 milligrams per liter) in the stream with current waste-discharge restrictions. During winter low flow, ammonia toxicity, rather than dissolved-oxygen concentration, was the limiting water-quality criterion downstream from the Salem wastewater-treatment facility. (USGS)

  11. A one-dimensional, steady-state, dissolved-oxygen model and waste-load assimilation study for South Fork, Wildcat Creek, Clinton County, Indiana

    USGS Publications Warehouse

    Crawford, Charles G.; Wilber, William G.; Peters, James G.

    1979-01-01

    The Indiana State Board of Health is developing a State water-quality management plan that includes establishing limits for wastewater effluents discharged into Indiana streams. A digital model calibrated to conditions in South Fork Wildcat Creek was used to predict alternatives for future waste loadings that would be compatible with Indiana stream water-quality standards defined for two critical hydrologic conditions, summer and winter low flows. Natural streamflow during the 7-day, 10-year low flow is zero, so no benefit from dilution is provided. The Indiana State Board of Health 's projected ammonia-nitrogen concentration for the Frankfort wastewater-treatment facility will violate the instream total ammonia-nitrogen standard of 2.5 mg/l and 4.0 mg/l during summer and winter low flows, respectively. The model indicates that nitrification and algal respiration were significant factors affecting the dissolved-oxygen dynamics of South Fork Wildcat Creek during two water-quality surveys. Stream water quality during the two water-quality surveys was degraded by the discharge of wastewater receiving only primary treatment. Benthic deposits resulting from this wastewater discharge seem to exert a considerable oxygen demand. The discharge of partially treated wastewater should be eliminated when a new wastewater-treatment facility becomes operational in mid-1979. Therefore, benthic-oxygen demand due to benthic deposits should become negligible at that time.

  12. Effect of the surfactant tween 80 on the detachment and dispersal of Salmonella enterica serovar Thompson single cells and aggregates from cilantro leaves as revealed by image analysis.

    PubMed

    Brandl, Maria T; Huynh, Steven

    2014-08-01

    Salmonella enterica has the ability to form biofilms and large aggregates on produce surfaces, including on cilantro leaves. Aggregates of S. enterica serovar Thompson that remained attached to cilantro leaves after rigorous washing and that were present free or bound to dislodged leaf tissue in the wash suspension were observed by confocal microscopy. Measurement of S. Thompson population sizes in the leaf washes by plate counts failed to show an effect of 0.05% Tween 80 on the removal of the pathogen from cilantro leaves 2 and 6 days after inoculation. On the contrary, digital image analysis of micrographs of single cells and aggregates of green fluorescent protein (GFP)-S. Thompson present in cilantro leaf washes revealed that single cells represented 13.7% of the cell assemblages in leaf washes containing Tween 80, versus 9.3% in those without the surfactant. Moreover, Tween 80 decreased the percentage of the total S. Thompson cell population located in aggregates equal to or larger than 64 cells from 9.8% to 4.4% (P < 0.05). Regression analysis of the frequency distribution of aggregate size in leaf washes with and without Tween 80 showed that the surfactant promoted the dispersal of cells from large aggregates into smaller ones and into single cells (P < 0.05). Our study underlines the importance of investigating bacterial behavior at the scale of single cells in order to uncover trends undetectable at the population level by bacterial plate counts. Such an approach may provide valuable information to devise strategies aimed at enhancing the efficacy of produce sanitization treatments. PMID:24907336

  13. A celebration of mechanics: from nano to macro. The J. Michael T. Thompson Festschrift issue

    PubMed Central

    Elishakoff, Isaac

    2013-01-01

    This Theme Issue is dedicated to the topic ‘Mechanics: from nano to macro’ and marks the 75th birthday of Dr J. Michael T. Thompson, Fellow of the Royal Society, whose current affiliations are as follows: (i) Honorary Fellow, Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge; (ii) Emeritus Professor of Nonlinear Dynamics, Department of Civil, Environmental and Geomatic Engineering, University College London; and (iii) Professor of Theoretical and Applied Dynamics (Distinguished Sixth Century Chair, part-time), University of Aberdeen. He also serves as Chairman of the Board of Directors at ES-Consult (consulting engineers) in Copenhagen, Denmark. The pertinent question that arises from the very start is: should we first salute Michael and then describe the Theme Issue, or vice versa? Indeed, according to Blaise Pascal (1623–1662), the last thing one discovers in composing a work is what to put first. I would like to take the liberty of deviating from the tradition of the Philosophical Transactions and start with the tribute to Michael; after all he is the prime cause of this Theme Issue. PMID:23690647

  14. A cost-benefit analysis of preventative management for zebra and quagga mussels in the Colorado-Big Thompson System

    USGS Publications Warehouse

    Thomas, Catherine M.

    2010-01-01

    net benefits of preventative management strategies. This study builds a bioeconomic simulation model to predict and compare the expected economic costs of the CDOW boat inspection program ot the benefits of reduced expected control costs to water conveyance systems, hydropower generation stations, and minicipal water treatment facilities. The model is based on a case study water delivery and storage system, the Colorado-Big Thompson system. The Colorado-Big Thomspon system is an excellent example of water systems in the Rocky Mountain West. The system is nearly entirely man-made, with all of its reservoirs and delivery points connected via pipelines, tunnels, and canals. The structures and hydropower systems of the Colorado-Big Thompson system are common to other western storage and delivery systems, making the methods and insight developed from this case study transferal to other western systems. The model developed in this study contributes to the bioeconomic literature in several ways. Foremost, the model predicts the spread of dreissena mussels and associated damage costs for a connected water system in the Rocky Mountain West. Very few zebra mussel studies have focused on western water systems. Another distinguishing factor is the simultaneous consideration of spread from propagules introduced by boats and by flows. Most zebra mussel dispersal models consider boater movement patterns combined with limnological characteristics as predictors of spread. A separate set of studies have addressed mussel spread via downstream flows. To the author's knowledge, this is the first study that builds a zebra mussel spread model that specifically accounts for propagule pressure from boat introductions and from downstream flow introductions. By modeling an entire connected system, the study highlights how the spatial layout of a system, and the risk of invasion within a system affect the benefits of preventative management. This report is presented in five chapters. The first

  15. Acoustic Doppler current profiling from the JGOFS Arabian Sea cruises aboard the RV T.G. THOMPSON: TN043, January 8, 1995--February 4, 1995; TN044, February 8, 1995--February 25, 1995; TN045, March 14, 1995--April 10, 1995; TN046, April 14, 1995--April 29, 1995

    SciTech Connect

    Flagg, C.N.; Kim, H.S.; Shi, Y.

    1995-09-01

    Acoustic Doppler current profiler (ADCP) data from the R/V T.G. THOMPSON is part of the core data for the US JGOFS Arabian Sea project along with hydrographic and nutrient data. Seventeen cruises on the THOMPSON are scheduled to take place between September 1994 and January 1996. This is the second in a series of data reports covering the ADCP data from the Arabian Sea JGOFS cruises TNO43 through TNO46. ADCP data are being collected on all the JGOFS Arabian Sea cruises using an autonomous data acquisition system developed for ship-of-opportunity cruises. This system, referred to as the AutoADCP, makes it possible to collect the ADCP data without the constant monitoring usually necessary and assures constant data coverage and uniform data quality. This data report presents ADCP results from the second group of four JGOFS cruises, TNO43 through TNO46, concentrating on the data collection and processing methods. The ADCP data itself reside in a CODAS data base at Brookhaven National Laboratory and is generally available to JGOFS investigators through contact with the authors. The CODAS data base and associated ADCP processing software were developed over a number of years by Eric Firing and his group at the University of Hawaii. The CODAS software is shareware available for PC`s or Unix computers and is the single most widely used ADCP processing program for ship mounted units.

  16. Flood-inundation maps for the East Fork White River at Columbus, Indiana

    USGS Publications Warehouse

    Lombard, Pamela J.

    2013-01-01

    Digital flood-inundation maps for a 5.4-mile reach of the East Fork White River at Columbus, Indiana, from where the Flatrock and Driftwood Rivers combine to make up East Fork White River to just upstream of the confluence of Clifty Creek with the East Fork White River, were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation, depict estimates of the areal extent of flooding corresponding to selected water levels (stages) at USGS streamgage 03364000, East Fork White River at Columbus, Indiana. Current conditions at the USGS streamgage may be obtained on the Internet from the USGS National Water Information System (http://waterdata.usgs.gov/in/nwis/uv/?site_no=03364000&agency_cd=USGS&). The National Weather Service (NWS) forecasts flood hydrographs for the East Fork White River at Columbus, Indiana at their Advanced Hydrologic Prediction Service (AHPS) flood warning system Website (http://water.weather.gov/ahps/), that may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. In this study, flood profiles were computed for the stream reach by means of a one-dimensional step-backwater model. The hydraulic model was calibrated by using the most current stage-discharge relation at USGS streamgage 03364000, East Fork White River at Columbus, Indiana. The calibrated hydraulic model was then used to determine 15 water-surface profiles for flood stages at 1-foot (ft) intervals referenced to the streamgage datum and ranging from bankfull to approximately the highest recorded water level at the streamgage. The simulated water-surface profiles were then combined with a geographic information system digital elevation model (derived from Light Detection and Ranging (LiDAR) data), having a 0.37-ft vertical accuracy and a 1.02 ft

  17. Using Caffeine as a Water Quality Indicator in the Ambient Monitoring Program for Third Fork Creek Watershed, Durham, North Carolina.

    PubMed

    Spence, Porché L

    2015-01-01

    Caffeine has been suggested as a chemical indicator for domestic wastewater in freshwater systems, although it is not included in water quality monitoring programs. The Third Fork Creek watershed in Durham, NC, is highly urbanized, with a history of receiving untreated wastewater from leaking and overflowing sanitary sewers. The poor water quality originating in the Third Fork Creek watershed threatens its intended uses and jeopardizes drinking water, aquatic life, and recreational activities provided by Jordan Lake. Organic waste contaminants have been detected in both Third Fork Creek watershed and Jordan Lake; however, the sampling periods were temporary, resulting in a few samples collected during nonstorm periods. It is recommended that (1) the concentration of caffeine and other organic waste contaminants are determined during storm and nonstorm periods and (2) caffeine is monitored regularly with traditional water quality indicators to evaluate the health of Third Fork Creek watershed. PMID:26157335

  18. TROUT DISTRIBUTION AND HABITAT IN RELATION TO GEOLOGY AND GEOMORPHOLOGY IN THE NORTH FORK HUMBOLDT RIVER DRAINAGE, NORTHEASTERN NEVADA

    EPA Science Inventory

    The authors studied the existing distribution of native Lahontan cutthroat trout Onocorpynchus clarki henshawi and exotic brook trout Salvelinus fontinalis with respect to geologic and geomorphic land-classes in the upper North Fork Humboldt River drainage, Nevada. hey evaluated ...

  19. Using Caffeine as a Water Quality Indicator in the Ambient Monitoring Program for Third Fork Creek Watershed, Durham, North Carolina

    PubMed Central

    Spence, Porché L

    2015-01-01

    Caffeine has been suggested as a chemical indicator for domestic wastewater in freshwater systems, although it is not included in water quality monitoring programs. The Third Fork Creek watershed in Durham, NC, is highly urbanized, with a history of receiving untreated wastewater from leaking and overflowing sanitary sewers. The poor water quality originating in the Third Fork Creek watershed threatens its intended uses and jeopardizes drinking water, aquatic life, and recreational activities provided by Jordan Lake. Organic waste contaminants have been detected in both Third Fork Creek watershed and Jordan Lake; however, the sampling periods were temporary, resulting in a few samples collected during nonstorm periods. It is recommended that (1) the concentration of caffeine and other organic waste contaminants are determined during storm and nonstorm periods and (2) caffeine is monitored regularly with traditional water quality indicators to evaluate the health of Third Fork Creek watershed. PMID:26157335

  20. 77 FR 39208 - Notice of Extension of Public Comment Period for Draft Environmental Impact Statement: North Fork...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-02

    ... supply water wells located on National Forest System (NFS) land in the North Fork Eagle Creek drainage... ] current adaptive management strategies which both respond to the purpose and need for action, and...

  1. CHARACTERIZATION OF MERCURY CONTAMINATION AT THE EAST FORK POPLAR CREEK SITE, OAK RIDGE, TENNESSEE: A CASE STUDY

    EPA Science Inventory

    Historic accidental release of mercury-contaminated material associated with nuclear weapons production at East Fork Poplar Creek (EFPC) resulted in stream and floodplain contamination. he EFPC is designated as an Oak Ridge Reservation (ORR) operable unit under the Comprehensive ...

  2. Evaluation of the Life History of Native Salmonids in the Malheur River Basin; Cooperative Bull Trout/Redband Trout Research Project, 1999-2000 Annual Report.

    SciTech Connect

    Schwabe, Lawrence; Tiley, Mark; Perkins, Raymond R.

    2000-11-01

    The purpose of this study is to document the seasonal distribution of adult/sub-adult bull trout (Salvelinus confluentus) in the Malheur River basin. Due to the decline of bull trout in the Columbia Basin, the United States Fish and Wildlife Service listed bull trout as a threatened species in June 1998. Past land management activities; construction of dams; and fish eradication projects in the North Fork and Middle Fork Malheur River by poisoning have worked in concert to cumulatively impact native species in the Malheur Basin (Bowers et. al. 1993). Survival of the remaining bull trout populations is severely threatened (Buchanan 1997). 1999 Research Objects are: (1) Document the migratory patterns of adult/sub-adult bull trout in the North Fork Malheur River; (2) Determine the seasonal bull trout use of Beulah Reservoir and bull trout entrainment; and (3) Timing and location of bull trout spawning in the North Fork Malheur River basin. The study area includes the Malheur basin from the mouth of the Malheur River located near Ontario, Oregon to the headwaters of the North Fork Malheur River (Map 1). All fish collected and most of the telemetry effort was done on the North Fork Malheur River subbasin (Map 2). Fish collection was conducted on the North Fork Malheur River at the tailwaters of Beulah Reservoir (RK 29), Beulah Reservoir (RK 29-RK 33), and in the North Fork Malheur River at Crane Crossing (RK 69) to the headwaters of the North Fork Malheur. Radio telemetry was done from the mouth of the Malheur River in Ontario, Oregon to the headwaters of the North Fork Malheur. This report will reflect all migration data collected from 3/1/99 to 12/31/99.

  3. Spatial separation of replisome arrest sites influences homologous recombination quality at a Tus/Ter-mediated replication fork barrier.

    PubMed

    Willis, Nicholas A; Scully, Ralph

    2016-07-17

    The Escherichia coli replication fork arrest complex Tus/Ter mediates site-specific replication fork arrest and homologous recombination (HR) on a mammalian chromosome, inducing both conservative "short tract" gene conversion (STGC) and error-prone "long tract" gene conversion (LTGC) products. We showed previously that bidirectional fork arrest is required for the generation of STGC products at Tus/Ter-stalled replication forks and that the HR mediators BRCA1, BRCA2 and Rad51 mediate STGC but suppress LTGC at Tus/Ter-arrested forks. Here, we report the impact of Ter array length on Tus/Ter-induced HR, comparing HR reporters containing arrays of 6, 9, 15 or 21 Ter sites-each targeted to the ROSA26 locus of mouse embryonic stem (ES) cells. Increasing Ter copy number within the array beyond 6 did not affect the magnitude of Tus/Ter-induced HR but biased HR in favor of LTGC. A "lock"-defective Tus mutant, F140A, known to exhibit higher affinity than wild type (wt)Tus for duplex Ter, reproduced these effects. In contrast, increasing Ter copy number within the array reduced HR induced by the I-SceI homing endonuclease, but produced no consistent bias toward LTGC. Thus, the mechanisms governing HR at Tus/Ter-arrested replication forks are distinct from those governing HR at an enzyme-induced chromosomal double strand break (DSB). We propose that increased spatial separation of the 2 arrested forks encountering an extended Tus/Ter barrier impairs the coordination of DNA ends generated by the processing of the stalled forks, thereby favoring aberrant LTGC over conservative STGC. PMID:27136113

  4. Hydrologic, water-quality, and biological characteristics of the North Fork Flathead River, Montana, water years 2007-2008

    USGS Publications Warehouse

    Mills, Taylor J.; Schweiger, E. William; Mast, M. Alisa; Clow, David W.

    2012-01-01

    In water year 2007, the U.S. Geological Survey, in cooperation with the National Park Service, began a 2-year study to collect hydrologic, water-quality, and biological data to provide a baseline characterization of the North Fork Flathead River from the United States-Canada border to its confluence with the Middle Fork of the Flathead River near Columbia Falls, Montana. Although mining in the Canadian portion of the North Fork Basin was banned in 2010 by a Memorandum of Understanding issued by the Province of British Columbia, baseline characterization was deemed important for the evaluation of any potential future changes in hydrology, water quality, or aquatic biology in the basin. The North Fork Basin above Columbia Falls (including Canada) drains an area of 1,564 square miles, and the study area encompasses the portion of the basin in Montana, which is 1,126 square miles. Seasonal patterns in the hydrology of the North Fork are dominated by the accumulation and melting of seasonal snowpack in the basin. Low-flow conditions occurred during the late-summer, fall, and winter months, and high-flow conditions coincided with the spring snowmelt. Substantial gains in streamflow occurred along the study reach of the North Fork, 85 percent of which were accounted for by tributary inflows during low-flow conditions, indicating unmeasured streamflow inputs along the main stem were 15 percent or less.

  5. The RecQ DNA helicase Rqh1 constrains Exonuclease 1-dependent recombination at stalled replication forks.

    PubMed

    Osman, Fekret; Ahn, Jong Sook; Lorenz, Alexander; Whitby, Matthew C

    2016-01-01

    DNA double-strand break (DSB) repair by homologous recombination (HR) involves resection of the break to expose a 3' single-stranded DNA tail. In budding yeast, resection occurs in two steps: initial short-range resection, performed by Mre11-Rad50-Xrs2 and Sae2; and long-range resection catalysed by either Exo1 or Sgs1-Dna2. Here we use genetic assays to investigate the importance of Exo1 and the Sgs1 homologue Rqh1 for DNA repair and promotion of direct repeat recombination in the fission yeast Schizosaccharomyces pombe. We find that Exo1 and Rqh1 function in alternative redundant pathways for promoting survival following replication fork breakage. Exo1 promotes replication fork barrier-induced direct repeat recombination but intriguingly limits recombination induced by fork breakage. Direct repeat recombination induced by ultraviolet light depends on either Exo1 or Rqh1. Finally, we show that Rqh1 plays a major role in limiting Exo1-dependent direct repeat recombination induced by replication fork stalling but only a minor role in constraining recombination induced by fork breakage. The implications of our findings are discussed in the context of the benefits that long-range resection may bring to processing perturbed replication forks. PMID:26957021

  6. The RecQ DNA helicase Rqh1 constrains Exonuclease 1-dependent recombination at stalled replication forks

    PubMed Central

    Osman, Fekret; Ahn, Jong Sook; Lorenz, Alexander; Whitby, Matthew C.

    2016-01-01

    DNA double-strand break (DSB) repair by homologous recombination (HR) involves resection of the break to expose a 3′ single-stranded DNA tail. In budding yeast, resection occurs in two steps: initial short-range resection, performed by Mre11-Rad50-Xrs2 and Sae2; and long-range resection catalysed by either Exo1 or Sgs1-Dna2. Here we use genetic assays to investigate the importance of Exo1 and the Sgs1 homologue Rqh1 for DNA repair and promotion of direct repeat recombination in the fission yeast Schizosaccharomyces pombe. We find that Exo1 and Rqh1 function in alternative redundant pathways for promoting survival following replication fork breakage. Exo1 promotes replication fork barrier-induced direct repeat recombination but intriguingly limits recombination induced by fork breakage. Direct repeat recombination induced by ultraviolet light depends on either Exo1 or Rqh1. Finally, we show that Rqh1 plays a major role in limiting Exo1-dependent direct repeat recombination induced by replication fork stalling but only a minor role in constraining recombination induced by fork breakage. The implications of our findings are discussed in the context of the benefits that long-range resection may bring to processing perturbed replication forks. PMID:26957021

  7. Response of the bacteriophage T4 replisome to non-coding lesions and regression of a stalled replication fork

    PubMed Central

    Nelson, Scott W.; Benkovic, Stephen J.

    2010-01-01

    DNA is constantly damaged by endogenous and exogenous agents. The resulting DNA lesions have the potential to halt the progression of the replisome, possibly leading to replication fork collapse. Here, we examine the effect of a non-coding DNA lesion in either the leading or lagging strand template on the bacteriophage T4 replisome. A damaged base in the lagging strand template does not affect the progression of the replication fork. Instead, the stalled lagging strand polymerase recycles from the lesion and initiates synthesis of the new Okazaki fragment upstream from the damaged base. In contrast, when the replisome encounters a blocking lesion in the leading strand template, the replication fork only travels approximately 1 kb beyond the point of the DNA lesion before complete replication fork collapse. The primosome and lagging strand polymerase remain active during this period and an Okazaki fragment is synthesized beyond the point of the leading strand lesion. There is no evidence for a new priming event on the leading strand template. Instead, the DNA structure that is produced by the stalled replication fork is a substrate for the DNA repair helicase, UvsW. UvsW catalyzes the regression of a stalled replication fork into a “chicken foot” structure that has been postulated to be an intermediate in an error-free lesion bypass pathway. PMID:20600127

  8. Backwater at bridges and densely wooded flood plains, west fork Amite River near Liberty, Mississippi

    USGS Publications Warehouse

    Colson, B.E.; Ming, C.O.; Arcement, George J.

    1979-01-01

    Floodflow data that will provide a base for evaluating digital models relating to open-channel flow were obtained at 22 sites on streams in Alabama, Louisiana, and Mississippi. Thirty-five floods were measured. Analysis of the data indicated methods currently in use would be inaccurate where densely vegetated flood plains are crossed by highway embankments and single-opening bridges. This atlas presents flood information at the site on West Fork Amite River near Liberty, MS. Water depths , velocities, and discharges through bridge openings on West Fork Amite River near Liberty, MS for floods of December 6, 1971 , and March 25, 1973, are shown, together with peak water-surface elevations along embankments and along cross sections. Manning 's roughness coefficient values in different parts of the flood plain are shown on maps, and flood-frequency relations are shown on a graph. (USGS).

  9. Landslide Sediment Production in the Middle Fork Eel River: 1940-2002

    NASA Astrophysics Data System (ADS)

    de La Fuente, J. A.; Snavely, W. P.; Miller, A. R.; Elder, D.

    2003-12-01

    A sequential air photo analysis was conducted by the U.S. Forest Service (USFS) and North State Resources in the 753 square mile Middle Fork Eel River basin, under contract to US Environmental Protection Agency as part of a Total Maximum Daily Load (TMDL) assessment. All landslides visible on air photos which appeared to deliver sediment to the stream system were mapped, and the delivered volume estimated. Landslides on undisturbed hillslopes were classified as natural, those within recently burned areas as fire-related, those in harvest units as harvest-related, and those adjacent to roads as road-related. About 5% of the landslides were field verified. Data were then summed for three air photo intervals, spanning the period 1940-2002. A total of 4,122 landslides were inventoried, delivering a total of 24,969,836 cubic yards of sediment to the stream system, and occupying 13,526 acres. The photo interval 1940-1969 accounted for 79% of the delivered volume, 1970-1984 for 8%, and 1985-2002 for 13%. This pattern is similar to that observed in many NW California watersheds where the storms of 1955 and 1964 generated large volumes of landslide sediment. Landslide volume per unit area delivered to streams from 1940-2002 averaged 0.84 cubic yards/acre/year for the Middle Fork Eel, and was highest in the Black Butte River, and Williams-Thatcher subwatersheds which exhibited rates of 1.55 and 1.31 cubic yards/acre/year respectively. The lowest delivery rates were in Upper Middle Fork (0.29), and Round Valley (0.44). Natural landslides accounted for 89.9% of the total, fire-related for 1.9%, harvest-related for 0.4%, road-related for 3.4%, and undetermined 4.4%. A large proportion of the total delivered sediment originated from the toes of large deep seated landslides adjacent to streams. Many of these landslides exhibited multiple years of activity, shedding debris slides of varying sizes at different times. The mapped active landslides are concentrated near streams, but

  10. Transition from laminar to turbulent drag in flow due to a vibrating quartz fork

    SciTech Connect

    Blazkova, M.; Schmoranzer, D.; Skrbek, L.

    2007-02-15

    Flow due to a commercially available vibrating quartz fork is studied in gaseous helium, He I and He II, over a wide range of temperatures and pressures. On increasing the driving force applied to the fork, the drag changes in character from laminar (characterized by a linear drive vs velocity dependence) to turbulent (characterized by a quadratic drive vs velocity dependence). We characterize this transition by a critical Reynolds number Re{sub cr}{sup {delta}}=U{sub cr}{delta}/{nu}, where U{sub cr} is the critical velocity, {nu} stands for the kinematic viscosity, {delta}={radical}(2{nu}/{omega}) is the viscous penetration depth, and {omega} is the angular frequency of oscillations. We have experimentally verified that the corresponding scaling U{sub cr}{proportional_to}{radical}({nu}{omega}) holds in a classical viscous fluid over two decades of {nu}.

  11. Thin-skinned shortening geometries of the South Fork fault: Bighorn basin, Park County, Wyoming

    SciTech Connect

    Clarey, T.L. )

    1990-01-01

    This paper presents a new interpretation of the South Fork fault in light of thin-skinned thrust theory. Cross sections and seismic data are presented which indicate that the South Fork fault is an allochthonous salient which was emplaced in the Bighorn basin during the early to middle Eocene. All observed structural geometries can be interpreted as developing under a compressional regime, similar to the Wyoming-Utah-Idaho thrust belt. Faults either follow bedding-plane surfaces, cut up section in the direction of tectonic transport or form backthrusts. A single decollement within the Jurassic Gypsum Spring Formation appears to dominate. Tectonic transport was approximately southeast, parallel to tear faults in the allochthonous plate.

  12. Orbital angular momentum entanglement via fork-poling nonlinear photonic crystals.

    PubMed

    Lu, L L; Xu, P; Zhong, M L; Bai, Y F; Zhu, S N

    2015-01-26

    We report a compact scheme for the generation and manipulation of photon pairs entangled in the orbital angular momentum (OAM) from the fork-poling quadratic nonlinear crystal. The χ(2)-modulation in this crystal is designed for fulfilling a tilted quasi-phase-matching geometry to ensure the efficient generation of entangled photons as well as for transferring of topological charge of the crystal to the photon pairs. Numerical results show that the OAM of photon pair is anti-correlated and the degree of OAM entanglement can be enhanced by modulating the topological charge of crystal, which indicates a feasible extension to high-dimensional OAM entanglement. These studies suggest that the fork-poling nonlinear photonic crystal a unique platform for compact generation and manipulation of high-dimensional and high-order OAM entanglement, which may have potential applications in quantum communication, quantum cryptography and quantum remote sensing. PMID:25835879

  13. Noncontact scanning force microscopy based on a modified tuning fork sensor

    NASA Astrophysics Data System (ADS)

    Göttlich, Hagen; Stark, Robert W.; Pedarnig, Johannes D.; Heckl, Wolfgang M.

    2000-08-01

    Distance control using a tuning fork setup for the detection of shear forces is a standard configuration in scanning near-field optical microscopy (SNOM). Based on this concept, a modified sensor was developed, where a standard silicon tip for atomic force microscopy (AFM) is attached to the front end of one prong of a 100 kHz quartz tuning fork oscillator. Comparison of force curves of a standard tapping-mode AFM cantilever, a conventional fiber tip SNOM sensor and the novel AFM tip shear force sensor demonstrate an enhanced stability and sensitivity of the new sensor. Due to the rigid sensor design the force curves of the AFM tip shear force sensor indicate a perfect noncontact behavior under normal conditions in air. Noncontact images show a comparable resolution to conventional force microscopy.

  14. Bend and refractive index sensing based on the tuning fork fiber

    NASA Astrophysics Data System (ADS)

    Sun, Bo; Li, Xuyou; Yu, Yingying; He, Kunpeng

    2015-08-01

    A fiber-optic based on the tuning fork structure is investigated for bend and refractive index (RI). The new bend/RI sensor based on the tuning fork structure is ease of fabrication, low cost, and simple signal acquisition. The operation principle relies on the power coupling of two cores inputted into light simultaneously. The beam-propagation method (BPM) is employed for modeling the propagation of light along the optical fiber sensing device proposed. The simulation results show that it exhibits very high sensitivity, accuracy and wide dynamic range in making curvature and RI measurements. The bending sensitivity is about 0.01184 W/m-1 at curvatures ranging from 0 to 50 m-1, the RI sensitivity is about -1.5557, -22.3031 and -102.44878 W/RIU at refractive indexes ranging from 1.33-1.418, 1.418-1.45 and 1.45-1.456, respectively.

  15. Experimental Study of Gyro Sensor Using Double-Ended Tuning Fork Quartz Resonator

    NASA Astrophysics Data System (ADS)

    Sato, Kenji; Ono, Atsushi; Tomikawa, Yoshiro

    2004-05-01

    In this study, we focus on a flatly supported gyro sensor using a double-ended tuning fork quartz resonator set in parallel with the rotating plane. The resonator has the advantages of flat form, high precision and strong shock resistance; moreover, fundamentally, the resonator is able to detect two-axial angular velocities. We clarified the features of the resonator by discussing the advantages and disadvantages of the resonator as a gyro sensor. The resonator was designed to have high detection efficiency, applying the vibration theory and using the finite element method. The resonator was finely fabricated by photolithography and wet etching. As a result, the resonators, without any problem as a gyro sensor, have been fabricated; we also confirmed experimentally that the practical angular velocity could be detected by the prototype gyro sensor. Consequently, we can conclude that the double-ended tuning fork quartz resonator could be used as the flatly supported gyro sensor.

  16. Acute MUS81 depletion leads to replication fork slowing and a constitutive DNA damage response

    PubMed Central

    Xing, Meichun; Wang, Xiaohui; Palmai-Pallag, Timea; Shen, Huahao; Helleday, Thomas; Hickson, Ian D.; Ying, Songmin

    2015-01-01

    The MUS81 protein belongs to a conserved family of DNA structure-specific nucleases that play important roles in DNA replication and repair. Inactivation of the Mus81 gene in mice has no major deleterious consequences for embryonic development, although cancer susceptibility has been reported. We have investigated the role of MUS81 in human cells by acutely depleting the protein using shRNAs. We found that MUS81 depletion from human fibroblasts leads to accumulation of ssDNA and a constitutive DNA damage response that ultimately activates cellular senescence. Moreover, we show that MUS81 is required for efficient replication fork progression during an unperturbed S-phase, and for recovery of productive replication following replication stalling. These results demonstrate essential roles for the MUS81 nuclease in maintenance of replication fork integrity. PMID:26415217

  17. Host DNA replication forks are not preferred targets for bacteriophage Mu transposition.

    PubMed Central

    Nakai, H; Taylor, A L

    1985-01-01

    Bacteriophage Mu DNA integration in Escherichia coli strains infected after alignment of chromosomal replication was analyzed by a sandwich hybridization assay. The results indicated that Mu integrated into chromosomal segments at various distances from oriC with similar kinetics. In an extension of these studies, various Hfr strains were infected after alignment of chromosomal replication, and Mu transposition was shut down early after infection. The positions of integrated Mu copies were inferred from the transfer kinetics of Mu to an F- strain. Our analysis indicated that the location of Mu DNA in the host chromosome was not dependent on the positions of host replication forks at the time of infection. However, the procedure for aligning chromosomal replication affected DNA transfer by various Hfr strains differently, and this effect could account for prior results suggesting preferential integration of Mu at host replication forks. Images PMID:3159718

  18. Final report for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils

    SciTech Connect

    1994-09-01

    IT Corporation (IT) was contracted by Martin Marietta Energy Systems, Inc. (Energy Systems) to perform a pilot-scale demonstration of the effectiveness of thermal desorption as a remedial technology for removing mercury from the Lower East Fork Poplar Creek (LEFPC) floodplain soil. Previous laboratory studies by Energy Systems suggested that this technology could reduce mercury to very low levels. This pilot-scale demonstration study was initiated to verify on an engineering scale the performance of thermal desorption. This report includes the details of the demonstration study, including descriptions of experimental equipment and procedures, test conditions, sampling and analysis, quality assurance (QA), detailed test results, and an engineering assessment of a conceptual full-scale treatment facility. The specific project tasks addressed in this report were performed between October 1993 and June 1994. These tasks include soil receipt, preparation, and characterization; prepilot (bench-scale) desorption tests; front-end materials handling tests; pilot tests; back-end materials handling tests; residuals treatment; and engineering scale-up assessment.

  19. Diel changes in stable carbon isotope ratios and trace element concentrations in the Clark Fork River, MT.

    NASA Astrophysics Data System (ADS)

    Parker, S.; Gammons, C.; Degrandpre, M.

    2004-12-01

    A diel (24-hr) water sampling was conducted on the Clark Fork River near Deer Lodge, Montana from 31-July to 1-August of 2003. The Clark Fork River is located in southwestern Montana and its upper reaches have been heavily affected by historic mining and smelting activities in the Butte and Anaconda areas. River floodplains and sediment beds contain significant quantities of metals (iron, aluminum, copper, zinc, lead, cadmium.) and arsenic. Two sites about 1.2 km apart, were sampled with a mean transit time for the water of 2.5 hours between the sites. The river in the study reach is characterized by oxic conditions, moderate alkalinity, moderate biological productivity and a pH range of about 8.0 to 8.5 during the summer low water months. During the mid-summer months, water in the Clark Fork River and its tributary streams is diverted for irrigation. Some of this water returns to the main stem in a chemically modified form as surface or groundwater return flow. This greatly complicates the hydrogeology and nutrient balance of the watershed. The two samplings sites used in this study make it possible to analyze the changes in chemical and physical properties of the water as it travels between the sites. Results reported here show that there is a large degree of temporal and spatial variability across the study area. In situ instruments were deployed and hourly water samples were collected for analysis from both sites. Diel concentration cycles are documented for dissolved forms of manganese, zinc and nitrate. Particulate forms of zinc, aluminum, copper, iron and manganese are shown to undergo twenty-four hour concentration changes. Total suspended solids (TSS) also show a diel change with the mass of TSS increasing at night. Dissolved arsenic is shown to undergo a diurnal concentration cycle at both sampling sites that was out of phase by the average 2.5 hour transit time between the two sites. This arsenic fluctuation may be a pulse of As that is being advected

  20. Initial Geomorphic Responses to Removal of Milltown Dam, Clark Fork River, Montana, USA

    NASA Astrophysics Data System (ADS)

    Wilcox, A. C.; Brinkerhoff, D.; Woelfle-Erskine, C.

    2008-12-01

    The removal of Milltown Dam on the Clark Fork River, Montana, USA, is creating a field-scale experiment on upstream and downstream responses to dam removal and on how gravel-bed rivers respond to sediment pulses. Milltown Dam was removed in 2008, reconnecting the Clark Fork River to its upstream basin in terms of sediment transport and fish passage. This dam removal is especially notable because (1) it is the largest dam removal to date in the United States in terms of the volume of reservoir sediment potentially available for downstream transport (over 3 million m3; 1.7 million m3 are being mechanically removed); and (2) the dam is the downstream end of the largest Superfund site in the United States, the Clark Fork Complex, and reservoir sediments are composed largely of contaminated mine tailings. Data collection on pre- and post-dam removal channel morphology, bed sediment characteristics, and sediment loads are being used to investigate spatial and temporal patterns of sediment transport and deposition associated with this dam removal. In the first several months following breaching of the dam, snowmelt runoff with a 3-year recurrence interval peak caused substantial erosion and downstream transport of metals-laden sediments from Milltown reservoir. Reservoir sediments in the Clark Fork arm of Milltown reservoir eroded at levels far exceeding modeling predictions as a result of both incision to the new base level created by dam removal and bank retreat of over 200 m in reaches upstream of a constructed bypass reach and remediation area. Copper and other metals in these eroded reservoir sediments provide a tracer for identifying whether sediment deposits observed downstream of the dam originated from Milltown reservoir or uncontaminated tributaries and indicate that Milltown sediments have reached over 200 km downstream. Downstream deposition has been greatest along channel margins and in side-channel areas, whereas the transport capacity of the active channel

  1. Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest.

    PubMed

    Bastia, Deepak; Srivastava, Pankaj; Zaman, Shamsu; Choudhury, Malay; Mohanty, Bidyut K; Bacal, Julien; Langston, Lance D; Pasero, Philippe; O'Donnell, Michael E

    2016-06-28

    Several important physiological transactions, including control of replicative life span (RLS), prevention of collision between replication and transcription, and cellular differentiation, require programmed replication fork arrest (PFA). However, a general mechanism of PFA has remained elusive. We previously showed that the Tof1-Csm3 fork protection complex is essential for PFA by antagonizing the Rrm3 helicase that displaces nonhistone protein barriers that impede fork progression. Here we show that mutations of Dbf4-dependent kinase (DDK) of Saccharomyces cerevisiae, but not other DNA replication factors, greatly reduced PFA at replication fork barriers in the spacer regions of the ribosomal DNA array. A key target of DDK is the mini chromosome maintenance (Mcm) 2-7 complex, which is known to require phosphorylation by DDK to form an active CMG [Cdc45 (cell division cycle gene 45), Mcm2-7, GINS (Go, Ichi, Ni, and San)] helicase. In vivo experiments showed that mutational inactivation of DDK caused release of Tof1 from the chromatin fractions. In vitro binding experiments confirmed that CMG and/or Mcm2-7 had to be phosphorylated for binding to phospho-Tof1-Csm3 but not to its dephosphorylated form. Suppressor mutations that bypass the requirement for Mcm2-7 phosphorylation by DDK restored PFA in the absence of the kinase. Retention of Tof1 in the chromatin fraction and PFA in vivo was promoted by the suppressor mcm5-bob1, which bypassed DDK requirement, indicating that under this condition a kinase other than DDK catalyzed the phosphorylation of Tof1. We propose that phosphorylation regulates the recruitment and retention of Tof1-Csm3 by the replisome and that this complex antagonizes the Rrm3 helicase, thereby promoting PFA, by preserving the integrity of the Fob1-Ter complex. PMID:27298353

  2. Selective Detection of Sulfur Derivatives Using Microfabricated Tuning Fork-Based Sensors

    PubMed Central

    Rai, Anant; Tsow, Francis; Nassirpour, Sanam; Bankers, Jeffrey; Spinatsch, Martina; He, M. Pete; Forzani, Erica; Tao, N. J.

    2009-01-01

    The paper describes an integtrated sensor system that can selectively and reversibly detect sulfur derivatives in the presence of interferent molecules. This is accomplished by integrating analyte-specific sensing materials with optimized filter materials. Microfabricated quartz tuning fork arrays are used to provide fast, accurate and low-cost transduction of the analyte binding events into electronic signals. The concept is demonstrated for detection of three sulfur derivatives – dimethyl disulfide, ethanethiol and methylsulfide. PMID:20160943

  3. Brief reconnaissance study for the addition of hydropower for Carr Fork Dam, Sassafras, Kentucky

    SciTech Connect

    Gebhard, T.G. Jr.

    1982-05-24

    The feasibility of retrofitting the Carr Fork Dam near Hazard, KY for power generation was examined. This dam has a developable head of 80 ft and was built in 1975 to provide flood protection. The study of environmental, institutional, safety, and economic factors showed that the total investment cost would be $909,600 and that hydroelectric power development at this site is not feasible unless a higher price could be obtained for the power sold. (LCL)

  4. 77 FR 66541 - Safety Zone; Alliance Road Bridge Demolition; Black Warrior River, Locust Fork; Birmingham, AL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ...The Coast Guard is establishing a temporary safety zone for a portion of the Locust Fork to the Black Warrior River, Birmingham, AL. This action is necessary for the protection of persons and vessels on navigable waters during the demolition of the Alliance Road Bridge (Co. Rd. 61). Entry into, transiting or anchoring in this zone is prohibited to all vessels, mariners, and persons unless......

  5. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress.

    PubMed

    Wilhelm, Therese; Ragu, Sandrine; Magdalou, Indiana; Machon, Christelle; Dardillac, Elodie; Técher, Hervé; Guitton, Jérôme; Debatisse, Michelle; Lopez, Bernard S

    2016-05-01

    Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es). Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3%) rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing), and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and senescence initiation

  6. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress

    PubMed Central

    Magdalou, Indiana; Machon, Christelle; Dardillac, Elodie; Técher, Hervé; Guitton, Jérôme; Debatisse, Michelle; Lopez, Bernard S.

    2016-01-01

    Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es). Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3%) rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing), and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and senescence initiation

  7. Characterizing water quality in the North Fork-Fall Creek Hydrologic Unit Area, Tennessee

    USGS Publications Warehouse

    Byl, Thomas Duane; Mattraw, H.C.

    1995-01-01

    The North Fork-Fall Creek Watershed in Bedford County, Tennessee is a karst terrain with a complex interconnection between ground water and the surface water-drainage network. Multiple sources of agricultural and domestic contamination make the effective design of best management practices difficult. Ongoing investigations by the U.S. Geological Survey and several county, State, and Federal agricultural agencies are attempting to refine source identification and improve the effectiveness of best management practices in the basin.

  8. Continuous hydrologic simulation of runoff for the Middle Fork and South Fork of the Beargrass Creek basin in Jefferson County, Kentucky

    USGS Publications Warehouse

    Jarrett, G. Lynn; Downs, Aimee C.; Grace-Jarrett, Patricia A.

    1998-01-01

    The Hydrological Simulation Pro-gram-FORTRAN (HSPF) was applied to an urban drainage basin in Jefferson County, Ky to integrate the large amounts of information being collected on water quantity and quality into an analytical framework that could be used as a management and planning tool. Hydrologic response units were developed using geographic data and a K-means analysis to characterize important hydrologic and physical factors in the basin. The Hydrological Simulation Program FORTRAN Expert System (HSPEXP) was used to calibrate the model parameters for the Middle Fork Beargrass Creek Basin for 3 years (June 1, 1991, to May 31, 1994) of 5-minute streamflow and precipitation time series, and 3 years of hourly pan-evaporation time series. The calibrated model parameters were applied to the South Fork Beargrass Creek Basin for confirmation. The model confirmation results indicated that the model simulated the system within acceptable tolerances. The coefficient of determination and coefficient of model-fit efficiency between simulated and observed daily flows were 0.91 and 0.82, respectively, for model calibration and 0.88 and 0.77, respectively, for model confirmation. The model is most sensitive to estimates of the area of effective impervious land in the basin; the spatial distribution of rain-fall; and the lower-zone evapotranspiration, lower-zone nominal storage, and infiltration-capacity parameters during recession and low-flow periods. The error contribution from these sources varies with season and antecedent conditions.

  9. Suboral fork: a newly discerned gnathosomal structure from the proboscis of eriophyoid mites (Acari, Eriophyoidea).

    PubMed

    Chetverikov, Philipp E; Bolton, Samuel

    2016-10-01

    The infracapitulum of eriophyoid mites comprises a cone-like basal infracapitulum, containing a pharynx, and a distal infracapitulum, forming a proboscis ensheathing a bunch of stylets. A well-developed basal labral section was observed in all studied specimens. A newly discerned structure, the suboral fork, situated in the ventral part of the proboscis was discovered. It is larger in diptilomiopids and Nalepella and notably smaller in eriophyids and phytoptids. This structure presumably determines the site of piercing and functions in a similar way to the pressure foot of a sewing machine which controls the movements of a needle. In diptilomiopids the suboral fork might have an additional function: it is a stopper which prevents the proboscis from further penetrating into plant tissues. It is possible that the suboral fork is homologous with the labium of early derivative acariform mites. The proboscis might be a fusion product of the infracapitular lateral lips, malapophyses and the labium. The proboscis serves as a feeding structure in eriophyoids; two ways of sucking plant cell sap, depending on shapes of proboscis and labrum, are hypothesized. Further work is needed to draw conclusions on homologies and the function of all gnathosomal structures in eriophyoids. PMID:27502114

  10. Brown trout avoidance of metals in water characteristic of the Clark Fork River, Montana

    USGS Publications Warehouse

    Woodward, Daniel F.; Hansen, James A.; Bergman, Harold L.; Delonay, Aaron J.; Little, Edward E.

    1995-01-01

    The avoidance response of brown trout (Salmo trutta) to mixtures of cadmium, copper, lead, and zinc was determined in water simulating the Clark Fork River, Montana. Effects of acidification on the avoidance response were also evaluated. Tests were conducted in a cylindrical chamber that received reference water at one end and metal-contaminated water at the other; a distinct boundary formed at the center where the chamber drained. A 1 × mixture of the four metals (Cd, 1.1 μg/L; Cu, 12 μg/L; Pb, 3.2 μg/L; and Zn, 55 μg/L) that was representative of the ambient metals concentrations of the Clark Fork River resulted in avoidance by brown trout. Brown trout also avoided 0.5×, 2×, 4×, and 10× mixtures but not a 0.1 × mixture. A reduction in pH from 8.0 to either 7.0, 6.0, or 5.0 resulted in significant avoidance. Avoidance reactions to metals, similar to those observed in our laboratory experiments, may contribute to the depression of brown trout populations in the Clark Fork River.

  11. Replication stalling at unstable inverted repeats: Interplay between DNA hairpins and fork stabilizing proteins

    PubMed Central

    Voineagu, Irina; Narayanan, Vidhya; Lobachev, Kirill S.; Mirkin, Sergei M.

    2008-01-01

    DNA inverted repeats (IRs) are hotspots of genomic instability in both prokaryotes and eukaryotes. This feature is commonly attributed to their ability to fold into hairpin- or cruciform-like DNA structures interfering with DNA replication and other genetic processes. However, direct evidence that IRs are replication stall sites in vivo is currently lacking. Here, we show by 2D electrophoretic analysis of replication intermediates that replication forks stall at IRs in bacteria, yeast, and mammalian cells. We found that DNA hairpins, rather than DNA cruciforms, are responsible for the replication stalling by comparing the effects of specifically designed imperfect IRs with varying lengths of their central spacer. Finally, we report that yeast fork-stabilizing proteins, Tof1 and Mrc1, are required to counteract repeat-mediated replication stalling. We show that the function of the Tof1 protein at DNA structure-mediated stall sites is different from its previously described effect on protein-mediated replication fork barriers. PMID:18632578

  12. Establishing a context for river rehabilitation, North Fork Gunnison River, Colorado.

    PubMed

    Jaquette, Christopher; Wohl, Ellen; Cooper, David

    2005-05-01

    Initial river rehabilitation efforts along the North Fork Gunnison River in Colorado focused on the use of in-stream structures and channel stabilization to create a single-thread channel with pools along a braided river. These efforts were based on the assumption that the river's braided planform results primarily from land use during the past century. In order to establish a context for further rehabilitation, we evaluated the possibility that the river might be braided as a result of processes independent of land use. We estimated volume, grain-size distribution, and lithology of sediment sources along the river corridor and evaluated the planform stability of the river during the past century using historical sources, aerial photographs covering 1939-1997, and comparison of bankfull discharge and gradient in the study area to values published for braided and meandering rivers. Our results indicate that the North Fork Gunnison River has been primarily braided in its lower reaches during the past few hundred years, although the channel planform tends toward a single-thread channel during decades of lower precipitation and discharge. Although land use is not the primary cause of braiding along the North Fork Gunnison River, it has decreased channel stability, and rehabilitation efforts should be designed to reduce these effects. Our results illustrate the importance of planning river rehabilitation measures within a historical context that accounts for both catchment-scale and reach-scale controls on channel processes and planform. PMID:15886956

  13. Far-travelled permian chert of the North Fork terrane, Klamath mountains, California

    USGS Publications Warehouse

    Mankinen, E.A.; Irwin, W.P.; Blome, C.D.

    1996-01-01

    Permian chert in the North Fork terrane and correlative rocks of the Klamath Mountains province has a remanent magnetization that is prefolding and presumably primary. Paleomagnetic results indicate that the chert formed at a paleolatitude of 8.6?? ?? 2.5?? but in which hemisphere remains uncertain. This finding requires that these rocks have undergone at least 8.6?? ?? 4.4?? of northward transport relative to Permian North America since their deposition. Paleontological evidence suggests that the Permian limestone of the Eastern Klamath terrane originated thousands of kilometers distant from North America. The limestone of the North Fork terrane may have formed at a similar or even greater distance as suggested by its faunal affinity to the Eastern Klamath terrane and more westerly position. Available evidence indicates that convergence of the North Fork and composite Central Metamorphic-Eastern Klamath terranes occurred during Triassic or Early Jurassic time and that their joining together was a Middle Jurassic event. Primary and secondary magnetizations indicate that the new composite terrane containing these and other rocks of the Western Paleozoic and Triassic belt behaved as a single rigid block that has been latitudinally concordant with the North American craton since Middle Jurassic time.

  14. Nek1 Regulates Rad54 to Orchestrate Homologous Recombination and Replication Fork Stability.

    PubMed

    Spies, Julian; Waizenegger, Anja; Barton, Olivia; Sürder, Michael; Wright, William D; Heyer, Wolf-Dietrich; Löbrich, Markus

    2016-06-16

    Never-in-mitosis A-related kinase 1 (Nek1) has established roles in apoptosis and cell cycle regulation. We show that human Nek1 regulates homologous recombination (HR) by phosphorylating Rad54 at Ser572 in late G2 phase. Nek1 deficiency as well as expression of unphosphorylatable Rad54 (Rad54-S572A) cause unresolved Rad51 foci and confer a defect in HR. Phospho-mimic Rad54 (Rad54-S572E), in contrast, promotes HR and rescues the HR defect associated with Nek1 loss. Although expression of phospho-mimic Rad54 is beneficial for HR, it causes Rad51 removal from chromatin and degradation of stalled replication forks in S phase. Thus, G2-specific phosphorylation of Rad54 by Nek1 promotes Rad51 chromatin removal during HR in G2 phase, and its absence in S phase is required for replication fork stability. In summary, Nek1 regulates Rad51 removal to orchestrate HR and replication fork stability. PMID:27264870

  15. On bandwidth characteristics of tuning fork micro-gyroscope with mechanically coupled sense mode.

    PubMed

    Ni, Yunfang; Li, Hongsheng; Huang, Libin; Ding, Xukai; Wang, Haipeng

    2014-01-01

    The bandwidth characteristics of a tuning fork micro-gyroscope with mechanically coupled sense mode were investigated in this paper to provide some references for mechanical bandwidth design. The concept of sense mode mechanical coupling is introduced first. Theoretical frequency response analyses were then carried out on the mechanical part of the gyroscope. Equations representing the relationships between the differential output signal and the frequency of the input angular rate were deduced in full frequency range and further simplified in low frequency range. Based on these equations, bandwidth characteristics under ideal and non-ideal conditions are discussed. Analytical results show that under ideal conditions, the bandwidth characteristics of a tuning fork micro-gyroscope are similar to those of a single mass micro-gyroscope, but under non-ideal conditions, especially when sense mass and/or stiffness are asymmetric, the bandwidth characteristics would be quite different because the in-phase mode would participate in the anti-phase vibration response. Experimental verifications were carried out on two micro-gyroscope prototypes designed in our laboratory. The deduced equations and analytical results can be used in guiding the mechanical bandwidth design of tuning fork micro-gyroscopes with mechanically coupled sense mode. PMID:25051030

  16. Mapping the Riverscape of the Middle Fork John Day River with Structure-from-Motion

    NASA Astrophysics Data System (ADS)

    Dietrich, J. T.

    2014-12-01

    Aerial photography has proven an efficient method to collect a wide range of continuous variables for large sections of rivers. These data include variables such as the planimetric shape, low-flow and bank-full widths, bathymetry, and sediment sizes. Mapping these variables in a continuous manner allows us to explore the heterogeneity of the river and build a more complete picture of the holistic riverscape. To explore a low-cost option for aerial photography and riverscape mapping, I used the combination of a piloted helicopter and an off-the-shelf digital SLR camera to collect aerial imagery for a 32 km segment of the Middle Fork John Day River in eastern Oregon. This imagery was processed with Structure-from-Motion (SfM) photogrammetry to produce high-resolution 10 cm orthophotos and digital surface models that were used to extract riverscape variables. The Middle Fork John Day River is an important spawning river for anadromous Chinnook and Steelhead and has been the focus of widespread restoration and conservation activities in response to the legacies of extensive grazing and mining activity. By mapping the riverscape of the Middle Fork John Day, I explored downstream relationships between several geomorphic variables with hyperscale analysis. These riverscape data also provided an opportunity to make a continuous map of habitat suitability for migrating adult Chinook. Both the geomorphic and habitat suitability analysis provide an important assessment of the natural variation in the river and the impact of human modification, both positive and negative.

  17. 77 FR 55796 - Sand Lick Fork Watershed Restoration Project; Daniel Boone National Forest, KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... soil loss by plugging abandoned oil wells, removing abandoned flow lines, restoration of stream... roads, has created areas of exposed soil and rutting, resulting in sedimentation of the streams and..., loosening of compacted soils, placement of woody debris from surrounding woodland, and planting with...

  18. DNA Replication Forks Pause at Silent Origins near the HML Locus in Budding Yeast

    PubMed Central

    Wang, Yangzhou; Vujcic, Marija; Kowalski, David

    2001-01-01

    Chromosomal replicators in budding yeast contain an autonomously replicating sequence (ARS) that functions in a plasmid, but certain ARSs are silent as replication origins in their natural chromosomal context. In chromosome III, the HML ARS cluster (ARS302-ARS303-ARS320) and ARS301 flank the transcriptionally silent mating-type locus HML, and all of these ARSs are silent as replication origins. ARS301 and ARS302 function in transcriptional silencing mediated by the origin recognition complex (ORC) and a heterochromatin structure, while the functions of ARS303 and ARS320 are not known. In this work, we discovered replication fork pause sites at the HML ARS cluster and ARS301 by analyzing DNA replication intermediates from the chromosome via two-dimensional gel electrophoresis. The replication fork pause at the HML ARS cluster was independent of cis- and trans-acting mutations that abrogate transcriptional silencing at HML. Deletion of the HML ARS cluster led to loss of the pause site. Insertion of a single, heterologous ARS (ARS305) in place of the HML ARS cluster reconstituted the pause site, as did multiple copies of DNA elements (A and B1) that bind ORC. The orc2-1 mutation, known to alter replication timing at origins, did not detectably affect the pause but activated the silent origin at the HML ARS cluster in a minority of cells. Delaying the time of fork arrival at HML led to the elimination of the pause sites at the HML ARS cluster and at the copy of ARS305 inserted in place of the cluster. Loss of the pause sites was accompanied by activation of the silent origins in the majority of cells. Thus, replication fork movement near HML pauses at a silent origin which is competent for replication initiation but kept silent through Orc2p, a component of the replication initiator. Possible functions for replication fork pause sites in checkpoints, S-phase regulation, mating-type switching, and transcriptionally silent heterochromatin are discussed. PMID:11438651

  19. Flood-inundation maps for the East Fork White River at Shoals, Indiana

    USGS Publications Warehouse

    Boldt, Justin A.

    2016-01-01

    Digital flood-inundation maps for a 5.9-mile reach of the East Fork White River at Shoals, Indiana (Ind.), were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Office of Community and Rural Affairs. The flood-inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the USGS streamgage on the East Fork White River at Shoals, Ind. (USGS station number 03373500). Near-real-time stages at this streamgage may be obtained on the Internet from the USGS National Water Information System at http://waterdata.usgs.gov/ or the National Weather Service (NWS) Advanced Hydrologic Prediction Service (AHPS) at http://water.weather.gov/ahps/, which also forecasts flood hydrographs at this site (NWS AHPS site SHLI3). NWS AHPS forecast peak stage information may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation.Flood profiles were computed for the East Fork White River reach by means of a one-dimensional, step-backwater model developed by the U.S. Army Corps of Engineers. The hydraulic model was calibrated by using the current stage-discharge relation (USGS rating no. 43.0) at USGS streamgage 03373500, East Fork White River at Shoals, Ind. The calibrated hydraulic model was then used to compute 26 water-surface profiles for flood stages at 1-foot (ft) intervals referenced to the streamgage datum and ranging from approximately bankfull (10 ft) to the highest stage of the current stage-discharge rating curve (35 ft). The simulated water-surface profiles were then combined with a geographic information system (GIS) digital elevation model (DEM), derived from light detection and ranging (lidar) data, to delineate the area flooded at each water level. The areal extent of the 24-ft flood-inundation map was

  20. Flood-inundation maps for the East Fork White River near Bedford, Indiana

    USGS Publications Warehouse

    Fowler, Kathleen K.

    2014-01-01

    Digital flood-inundation maps for an 1.8-mile reach of the East Fork White River near Bedford, Indiana (Ind.) were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent and depth of flooding corresponding to selectedwater levels (stages) at USGS streamgage 03371500, East Fork White River near Bedford, Ind. Current conditions for estimating near-real-time areas of inundation using USGS streamgage information may be obtained on the Internet at http://waterdata.usgs.gov/in/nwis/uv?site_no=03371500. In addition, information has been provided to the National Weather Service (NWS) for incorporation into their Advanced Hydrologic Prediction Service (AHPS) flood warning system (http://water.weather.gov/ahps/). The NWS forecasts flood hydrographs at many places that are often colocated with USGS streamgages, including the East Fork White River near Bedford, Ind. NWS-forecasted peak-stage information may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. For this study, flood profiles were computed for the East Fork White River reach by means of a one-dimensional step-backwater model. The hydraulic model was calibrated by using the most current stage-discharge relations at USGS streamgage 03371500, East Fork White River near Bedford, Ind., and documented high-water marks from the flood of June 2008. The calibrated hydraulic model was then used to determine 20 water-surface profiles for flood stages at 1-foot intervals referenced to the streamgage datum and ranging from bankfull to the highest stage of the current stage-discharge rating curve. The simulated water-surface profiles were then combined with a geographic information system (GIS) digital elevation model (DEM, derived from

  1. Late Cretaceous Middle Fork caldera and its resurgent granite porphyry intrusion, east-central Alaska

    NASA Astrophysics Data System (ADS)

    Bacon, C. R.; Dusel-Bacon, C.; Aleinikoff, J. N.; Slack, J. F.

    2012-12-01

    Named for the Middle Fork of the North Fork of the Fortymile River, the Middle Fork caldera encompasses a 10 x 20 km area of rhyolite welded tuff and granite porphyry ~100 km west of the Yukon border. Intracaldera tuff has ≤4 mm quartz and feldspar phenocrysts and cm-sized fiamme; its maximum exposed thickness is 850 m. Less densely welded tuff near the caldera margins locally contains 1-2 cm K-feldspar megacrysts and pumice clasts to 6 cm. Zircon from intracaldera tuff yields a SHRIMP-RG U-Pb age of 68.7 ± 1.1 Ma (all ages 95% confidence). Granite porphyry occupies much of an 8 x 12 km area having 650 m of relief within the western part of the caldera fill. Zircon from the porphyry gives a SHRIMP-RG U-Pb age of 68.4 ± 1.0 Ma. These ages agree with a previous 40Ar/39Ar biotite age of 69.1 ± 0.5 Ma for proximal outflow tuff. The crystal-rich intracaldera tuff contains embayed quartz, plagioclase>K-feldspar, biotite, and Fe-Ti oxide phenocrysts in a very fine-grained crystalline groundmass. The porphyry carries 40-50% of larger phenocrysts of the same phases (skeletal quartz to 1 cm; K-feldspar to 2 cm, rarely to 4 cm) in a fine-grained groundmass characterized by abundant 50-100 μm quartz. Compositions of 3 tuff and 3 porphyry samples overlap, form a limited differentiation series at 69-72% SiO2, have arc geochemical signatures, and yield subparallel chondrite-normalized rare earth element patterns with light REE enrichment, concave-upward heavy REE, and small negative Eu anomalies. Although their phenocrysts differ in size (owing to fragmentation of crystals in the tuff) and abundance, the similar mineralogy, composition (in spite of crystal concentration in the tuff), and indistinguishable ages of the tuff and porphyry indicate that the magmas were closely related. A rare magmatic enclave (54% SiO2, arc geochemical signature) in the porphyry may be similar to parental magma and provides evidence of mafic magma and thermal input. The porphyry is interpreted

  2. DNA-PKcs and PARP1 Bind to Unresected Stalled DNA Replication Forks Where They Recruit XRCC1 to Mediate Repair.

    PubMed

    Ying, Songmin; Chen, Zhihui; Medhurst, Annette L; Neal, Jessica A; Bao, Zhengqiang; Mortusewicz, Oliver; McGouran, Joanna; Song, Xinming; Shen, Huahao; Hamdy, Freddie C; Kessler, Benedikt M; Meek, Katheryn; Helleday, Thomas

    2016-03-01

    A series of critical pathways are responsible for the detection, signaling, and restart of replication forks that encounter blocks during S-phase progression. Small base lesions may obstruct replication fork progression and processing, but the link between repair of small lesions and replication forks is unclear. In this study, we investigated a hypothesized role for DNA-PK, an important enzyme in DNA repair, in cellular responses to DNA replication stress. The enzyme catalytic subunit DNA-PKcs was phosphorylated on S2056 at sites of stalled replication forks in response to short hydroxyurea treatment. Using DNA fiber experiments, we found that catalytically active DNA-PK was required for efficient replication restart of stalled forks. Furthermore, enzymatically active DNA-PK was also required for PARP-dependent recruitment of XRCC1 to stalled replication forks. This activity was enhanced by preventing Mre11-dependent DNA end resection, suggesting that XRCC1 must be recruited early to an unresected stalled fork. We also found that XRCC1 was required for effective restart of a subset of stalled replication forks. Overall, our work suggested that DNA-PK and PARP-dependent recruitment of XRCC1 is necessary to effectively protect, repair, and restart stalled replication forks, providing new insight into how genomic stability is preserved. Cancer Res; 76(5); 1078-88. ©2015 AACR. PMID:26603896

  3. Effects of controlled burning of chaparral on streamflow and sediment characteristics, East Fork Sycamore Creek, central Arizona

    USGS Publications Warehouse

    Baldys, Stanley; Hjalmarson, H.W.

    1994-01-01

    The effects of controlled burning of part of a chaparral-covered drainage basin on streamflow and sediment characteristics were studied in the upper reaches of the Sycamore Creek basin in central Arizona. A paired-watershed method was used to analyze data collected in two phases separated by the controlled burning of 45 percent of the East Fork Sycamore Creek drainage basin by the U.S. Forest Service on October 31, 1981. Statistically significant increases in streamflow in East Fork occurred from October 26, 1982, through August 25, 1984. Streamflow for August 26, 1984, through the end of data collection for the study on May 31, 1986, was generally at or less than preburn levels. An increase in the percentage of time that flow occurred in East Fork was noted for water years 1983 and 1984. No increase in the magnitude of instantaneous peak flows as a result of the burn was discernable at statistically significant levels. Suspended-sediment yields computed for data collected during water year 1983 were significantly greater in the East Fork drainage basin, 546 tons per square mile, than in the West Fork drainage basin, 22.6 tons per square mile. Suspended-sediment yields computed for East Fork and West Fork for water year 1985, 38.3 and 13.3 tons per square mile, respectively, were much closer in yield. These more uniform yields indicate a possible return to preburn conditions. Data collection did not begin until 11 months after the burn; therefore, the largest increases in streamflow and sediment yields, which commonly occur during the year after a burn, may not have been measured. During the second through fourth years after the burn, smaller increases in stream- flow and sediment yields were found in this study than were found in similar studies in this region.

  4. A retrospective study of the chemical analysis cost for the remediation of Lower East Fork Poplar Creek, Oak Ridge, Tennessee

    SciTech Connect

    Klatt, L.N.

    1998-06-01

    A retrospective study of the remediation of Lower East Fork Poplar Creek (LEFPC) in Oak Ridge, Tennessee was completed. The study was conducted by reviewing the public Comprehensive Environmental Response, Compensation, and Liability Act record documents associated with the remediation of LEFPC and through discussions with the project staff involved or familiar with the project. The remediation took place in two phases. The first phase involved the excavation of about 5,560 yd{sup 3} of soil at the National Oceanic and Atmospheric Administration (NOAA) locations in 1996. The second phase involved the excavation of 39,200 yd{sup 3} at another NOAA location and at the Bruner location in 1997. For the entire project (remedial investigation through cleanup), a total of 7,708 samples (1 sample for each 5.8 yd{sup 3} of soil remediated) were analyzed for mercury. The project obtained special regulatory approval to use two methods for the determination of mercury in soils that are not part of the Resource Conservation and Recovery Act SW-846 methods manual. The mercury analysis cost was $678,000, which represents 9.6% of the cleanup cost. During the cleanup phase of the project, an on-site laboratory was used. The estimated cost savings that the on-site laboratory provided fall into two categories: direct reduction of costs associated with chemical analysis and sample shipment totaling approximately $38,000, which represents a 5.3% savings relative to the estimated cost of using an off-site laboratory, and savings in the amount of $890,000 (12.5% of the $7.1 M cleanup cost), associated with expediting execution of the cleanup work by providing rapid (< 3 hours) sample result turnaround time. The manner in which the analytical services were procured for the LEFPC project suggest that the development of new chemical analysis technology must address deployment, performance, regulatory, robustness, reliability, and business appropriateness factors if the technology is to be

  5. Kevorkian v. Thompson.

    PubMed

    1997-01-01

    The United States District Court of the Eastern District of Michigan, 6 January 1997 [date of decision], held that a mentally competent patient who is terminally ill or intractably suffering does not have a liberty interest in assisted suicide under the due process clause of the Fourth Amendment and is not denied equal protection under the Fourteenth Amendment. Plaintiff Jack Kevorkian is a physician who advocates the right to die and assisted patients to commit suicide. Plaintiff Janet Good, the former president of the Michigan Hemlock Society, suffers from terminal pancreatic cancer. The plaintiffs claimed that Michigan's statute prohibiting physician-assisted suicide is unconstitutional. The District Court held there is no cognizable constitutional right to assisted suicide because the right to suicide or assisted suicide is not deeply rooted in the nation's history and traditions, and because the statute does not infringe on any fundamental right or liberty. The court noted a difference between the withdrawal of life support and acts to hasten death by assisted suicide, and further held that the law against physician-assisted suicide furthered legitimate state interests in denying to physicians "the role of killers of their patients," in regulating circumstances under which life may be ended, and in protecting the vulnerable but viable from "self-interested importuning of third parties." PMID:16285100

  6. Kevorkian v. Thompson.

    PubMed

    1997-01-01

    Court Decision: 947 Federal Supplement 1152; 1997 Jan 6 (date of decision). The United States District Court of the Eastern District of Michigan held that a mentally competent patient who is terminally ill or intractably suffering does not have a liberty interest in assisted suicide under the due process clause of the Fourth Amendment and is not denied equal protection under the Fourteenth Amendment. Plaintiff Jack Kevorkian is a physician who advocates the right to die and assisted patients to commit suicide. Plaintiff Janet Good, the former president of the Michigan Hemlock Society, suffers from terminal pancreatic cancer. The plaintiffs claimed that Michigan's statute prohibiting physician-assisted suicide is unconstitutional. The District Court held that there is no cognizable constitutional right to assisted suicide because the right to suicide or assisted suicide is not deeply rooted in the nation's history and traditions, and because the statute does not infringe on any fundamental right or liberty. The court noted a difference between the withdrawal of life support and acts to hasten death by assisted suicide. The court also held that the law against physician-assisted suicide furthered legitimate state interests in denying to physicians "the role of killers of their patients," in regulating circumstances under which life may be ended, and in protecting the vulnerable but viable from "self-interested importuning of third parties." PMID:16320473

  7. The Late Cretaceous Middle Fork caldera, its resurgent intrusion, and enduring landscape stability in east-central Alaska

    USGS Publications Warehouse

    Bacon, Charles R.; Dusel-Bacon, Cynthia; Aleinikoff, John N.; Slack, John F.

    2014-01-01

    The Middle Fork is a relatively well preserved caldera within a broad region of Paleozoic metamorphic rocks and Mesozoic plutons bounded by northeast-trending faults. In the relatively downdropped and less deeply exhumed crustal blocks, Cretaceous–Early Tertiary silicic volcanic rocks attest to long-term stability of the landscape. Within the Middle Fork caldera, the granite porphyry is interpreted to have been exposed by erosion of thick intracaldera tuff from an asymmetric resurgent dome. The Middle Fork of the North Fork of the Fortymile River incised an arcuate valley into and around the caldera fill on the west and north and may have cut down from within an original caldera moat. The 70 Ma land surface is preserved beneath proximal outflow tuff at the west margin of the caldera structure and beneath welded outflow tuff 16–23 km east-southeast of the caldera in a paleovalley. Within ∼50 km of the Middle Fork caldera are 14 examples of Late Cretaceous (?)–Tertiary felsic volcanic and hypabyssal intrusive rocks that range in area from <1 km2 to ∼100 km2. Rhyolite dome clusters north and northwest of the caldera occupy tectonic basins associated with northeast-trending faults and are relatively little eroded. Lava of a latite complex, 12–19 km northeast of the caldera, apparently flowed into the paleovalley of the Middle Fork of the North Fork of the Fortymile River. To the northwest of the Middle Fork caldera, in the Mount Harper crustal block, mid-Cretaceous plutonic rocks are widely exposed, indicating greater total exhumation. To the southeast of the Middle Fork block, the Mount Veta block has been uplifted sufficiently to expose a ca. 68–66 Ma equigranular granitic pluton. Farther to the southeast, in the Kechumstuk block, the flat-lying outflow tuff remnant in Gold Creek and a regionally extensive high terrace indicate that the landscape there has been little modified since 70 Ma other than entrenchment of tributaries in response to post–2

  8. Hydrology of the North Fork of the Right Fork of Miller Creek, Carbon County, Utah, before, during, and after underground coal mining

    USGS Publications Warehouse

    Slaughter, C.B.; Freethey, G.W.; Spangler, L.E.

    1995-01-01

    From 1988-92 the U.S. Geological Survey, in cooperation with the Utah Division of Oil, Gas, and Mining, studied the effects of underground coal mining and the resulting subsidence on the hydrologic system near the North Fork of the Right Fork of Miller Creek, Carbon County, Utah. The subsidence caused open fractures at land surface, debris slides, and rockfalls in the canyon above the mined area. Land surface subsided and moved several feet horizontally. The perennial stream and a tributary upstream from the mined area were diverted below the ground by surface fractures where the overburden thickness above the Wattis coal seam is 300 to 500 feet. The reach downstream was dry but flow resumed where the channel traversed the Star Point Sandstone, which forms the aquifer below the coal seams where ground-water discharge provides new base flow. Concentrations of dissolved constituents in the stream water sampled just downstream from the mined area increased from about 300 mg/L (milligrams per liter) to more than 1,500 mg/L, and the water changed from primarily a magnesium calcium bicarbonate to primarily a magnesium sulfate type. Monitored water levels in two wells completed in the perched aquifer(s) above the mine indicate that fractures from subsidence- related deformation drained the perched aquifer in the Blackhawk Formation. The deformation also could have contributed to the decrease in discharge of three springs above the mined area, but discharge from other springs in the area did not change ubstantially; thus, the relation between subsidence and spring discharge, if any, is not clear. No significant changes in the chemical character of water discharging from springs were detected, but the dissolved-solids concentration in water collected from a perched sandstone aquifer overlying the mined coal seams increased during mining activity.

  9. Replication fork progression is paused in two large chromosomal zones flanking the DNA replication origin in Escherichia coli.

    PubMed

    Akiyama, Masahiro Tatsumi; Oshima, Taku; Chumsakul, Onuma; Ishikawa, Shu; Maki, Hisaji

    2016-08-01

    Although the speed of nascent DNA synthesis at individual replication forks is relatively uniform in bacterial cells, the dynamics of replication fork progression on the chromosome are hampered by a variety of natural impediments. Genome replication dynamics can be directly measured from an exponentially growing cell population by sequencing newly synthesized DNA strands that were specifically pulse-labeled with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). However, a short pulse labeling with BrdU is impracticable for bacteria because of poor incorporation of BrdU into the cells, and thus, the genomewide dynamics of bacterial DNA replication remain undetermined. Using a new thymidine-requiring Escherichia coli strain, eCOMB, and high-throughput sequencing, we succeeded in determining the genomewide replication profile in bacterial cells. We also found that fork progression is paused in two ~200-kb chromosomal zones that flank the replication origin in the growing cells. This origin-proximal obstruction to fork progression was overcome by an increased thymidine concentration in the culture medium and enhanced by inhibition of transcription. These indicate that DNA replication near the origin is sensitive to the impediments to fork progression, namely a scarcity of the DNA precursor deoxythymidine triphosphate and probable conflicts between replication and transcription machineries. PMID:27353572

  10. Calibration of quartz tuning fork spring constants for non-contact atomic force microscopy: direct mechanical measurements and simulations

    PubMed Central

    Langewisch, Gernot; Schurig, Philipp; Hölscher, Hendrik; Fuchs, Harald; Schirmeisen, André

    2014-01-01

    Summary Quartz tuning forks are being increasingly employed as sensors in non-contact atomic force microscopy especially in the “qPlus” design. In this study a new and easily applicable setup has been used to determine the static spring constant at several positions along the prong of the tuning fork. The results show a significant deviation from values calculated with the beam formula. In order to understand this discrepancy the complete sensor set-up has been digitally rebuilt and analyzed by using finite element method simulations. These simulations provide a detailed view of the strain/stress distribution inside the tuning fork. The simulations show quantitative agreement with the beam formula if the beam origin is shifted to the position of zero stress onset inside the tuning fork base and torsional effects are also included. We further found significant discrepancies between experimental calibration values and predictions from the shifted beam formula, which are related to a large variance in tip misalignment during the tuning fork assembling process. PMID:24778977

  11. Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation

    NASA Astrophysics Data System (ADS)

    Min, Wookee; Bruhn, Christopher; Grigaravicius, Paulius; Zhou, Zhong-Wei; Li, Fu; Krüger, Anja; Siddeek, Bénazir; Greulich, Karl-Otto; Popp, Oliver; Meisezahl, Chris; Calkhoven, Cornelis F.; Bürkle, Alexander; Xu, Xingzhi; Wang, Zhao-Qi

    2013-12-01

    Damaged replication forks activate poly(ADP-ribose) polymerase 1 (PARP1), which catalyses poly(ADP-ribose) (PAR) formation; however, how PARP1 or poly(ADP-ribosyl)ation is involved in the S-phase checkpoint is unknown. Here we show that PAR, supplied by PARP1, interacts with Chk1 via a novel PAR-binding regulatory (PbR) motif in Chk1, independent of ATR and its activity. iPOND studies reveal that Chk1 associates readily with the unperturbed replication fork and that PAR is required for efficient retention of Chk1 and phosphorylated Chk1 at the fork. A PbR mutation, which disrupts PAR binding, but not the interaction with its partners Claspin or BRCA1, impairs Chk1 and the S-phase checkpoint activation, and mirrors Chk1 knockdown-induced hypersensitivity to fork poisoning. We find that long chains, but not short chains, of PAR stimulate Chk1 kinase activity. Collectively, we disclose a previously unrecognized mechanism of the S-phase checkpoint by PAR metabolism that modulates Chk1 activity at the replication fork.

  12. Mercury and other Mining-Related Contaminants in Ospreys along the Upper Clark Fork River, MT

    NASA Astrophysics Data System (ADS)

    Langner, H.; Domenech, R.; Greene, E.; Staats, M. F.

    2010-12-01

    Osprey (Pandion haliaetus) are widely recognized as bio-indicators of the health of aquatic ecosystems. Until the time of fledging, nestlings feed exclusively on fish caught within a few kilometers of the nest. Therefore, tissues of these young birds may reflect the level of contamination of local fish and more generally, the contamination status of the aquatic ecosystem they inhabit. Nests can often be accessed with a boom truck and obtaining small blood samples from the flightless chicks is fairly noninvasive. Ospreys are nesting along the Upper Clark Fork River, Montana, which is heavily contaminated with wastes left from a century of copper and precious metals mining. We have been monitoring the levels of priority pollutants (arsenic, cadmium, lead, copper, zinc, mercury and selenium) in Osprey chicks along a 250 km section of the river for four years. Objectives are to establish current contaminant status, pinpoint pollution hotspots, and assess the success of restoration efforts. Our results suggest that of highest concern may be the bioaccumulation of mercury with blood levels of up to 0.7 mg/L in the growing chicks. These concentrations are expected to increase many fold upon fledging as feather growth stops, which acts as the major sink for mercury. Interestingly, we found mercury levels increased in downstream direction, in contrast to concentrations of other pollutants. Reasons may be the different origin of mercury versus other contaminants and the distribution of wetlands where mercury can be transformed into highly bioavailable methylmercury. Blood levels of selenium are also elevated throughout the Upper Clark Fork River drainage. We discuss the implications for restoration and remediation of the Clark Fork River.

  13. In-Field Performance Testing of the Fork Detector for Quantitative Spent Fuel Verification

    SciTech Connect

    Gauld, Ian C.; Hu, Jianwei; De Baere, P.; Vaccaro, S.; Schwalbach, P.; Liljenfeldt, Henrik; Tobin, Stephen

    2015-01-01

    Expanding spent fuel dry storage activities worldwide are increasing demands on safeguards authorities that perform inspections. The European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) require measurements to verify declarations when spent fuel is transferred to difficult-to-access locations, such as dry storage casks and the repositories planned in Finland and Sweden. EURATOM makes routine use of the Fork detector to obtain gross gamma and total neutron measurements during spent fuel inspections. Data analysis is performed by modules in the integrated Review and Analysis Program (iRAP) software, developed jointly by EURATOM and the IAEA. Under the framework of the US Department of Energy–EURATOM cooperation agreement, a module for automated Fork detector data analysis has been developed by Oak Ridge National Laboratory (ORNL) using the ORIGEN code from the SCALE code system and implemented in iRAP. EURATOM and ORNL recently performed measurements on 30 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel (Clab), operated by the Swedish Nuclear Fuel and Waste Management Company (SKB). The measured assemblies represent a broad range of fuel characteristics. Neutron count rates for 15 measured pressurized water reactor assemblies are predicted with an average relative standard deviation of 4.6%, and gamma signals are predicted on average within 2.6% of the measurement. The 15 measured boiling water reactor assemblies exhibit slightly larger deviations of 5.2% for the gamma signals and 5.7% for the neutron count rates, compared to measurements. These findings suggest that with improved analysis of the measurement data, existing instruments can provide increased verification of operator declarations of the spent fuel and thereby also provide greater ability to confirm integrity of an assembly. These results support the application of the Fork detector as a fully quantitative spent fuel

  14. Saccharomyces cerevisiae Genetics Predicts Candidate Therapeutic Genetic Interactions at the Mammalian Replication Fork

    PubMed Central

    van Pel, Derek M.; Stirling, Peter C.; Minaker, Sean W.; Sipahimalani, Payal; Hieter, Philip

    2013-01-01

    The concept of synthetic lethality has gained popularity as a rational guide for predicting chemotherapeutic targets based on negative genetic interactions between tumor-specific somatic mutations and a second-site target gene. One hallmark of most cancers that can be exploited by chemotherapies is chromosome instability (CIN). Because chromosome replication, maintenance, and segregation represent conserved and cell-essential processes, they can be modeled effectively in simpler eukaryotes such as Saccharomyces cerevisiae. Here we analyze and extend genetic networks of CIN cancer gene orthologs in yeast, focusing on essential genes. This identifies hub genes and processes that are candidate targets for synthetic lethal killing of cancer cells with defined somatic mutations. One hub process in these networks is DNA replication. A nonessential, fork-associated scaffold, CTF4, is among the most highly connected genes. As Ctf4 lacks enzymatic activity, potentially limiting its development as a therapeutic target, we exploited its function as a physical interaction hub to rationally predict synthetic lethal interactions between essential Ctf4-binding proteins and CIN cancer gene orthologs. We then validated a subset of predicted genetic interactions in a human colorectal cancer cell line, showing that siRNA-mediated knockdown of MRE11A sensitizes cells to depletion of various replication fork-associated proteins. Overall, this work describes methods to identify, predict, and validate in cancer cells candidate therapeutic targets for tumors with known somatic mutations in CIN genes using data from yeast. We affirm not only replication stress but also the targeting of DNA replication fork proteins themselves as potential targets for anticancer therapeutic development. PMID:23390603

  15. 'A way of struggle': reformations and affirmations of E. P. Thompson's class analysis in the light of postmodern theories of language.

    PubMed

    Steinberg, M W

    1997-09-01

    This paper is an analysis of the role of language in historical class formation in light of the recent developments in postmodern social theory and historiography. Revisionists from within this perspective have questioned if not abandoned E. P. Thompson's class struggle analysis, arguing that he fails to account for the constitutive character of language in the construction of collective identities. They oppose his account of the making of the English working class with alternative histories emphasizing populist and other non-class identities. Drawing on the Bakhtin Circle of literary studies, and returning to Thompson's own writings, I argue that we can incorporate language into class struggle analysis as a critical mediating force. I maintain that class struggle occurs largely within a hegemonic discursive formation, and that class consciousness and identity thus in part are formed through counter-hegemonic strategies of resistance to ideological domination. To illustrate this theory I analyse the role of language in the class struggles of the silk weavers of the Spitalfields district in London in the 1820s. I analyse how the silk weavers articulated a class consciousness through their counter-hegemonic struggles with the large capitalists and the language of political economy. PMID:9372638

  16. An identification of the East Fork Poplar Creek floodplain, Anderson and Roane Counties, Tennessee

    SciTech Connect

    1992-12-01

    The work in this report was conducted by the US Army Corps of Engineers, Nashville District, during the period November 1991 through July 1992. The purpose of this study is to identify the East Fork Poplar Creek (EFPC) floodplain. This information is required as part of the remedial action plans for removal or containment of contamination within the EFPC floodplain. EFPC and a portion of its floodplain have been contaminated as a result of operations and accidental releases at the Department of Energy`s Y-12 Plant. Mercury is the major contaminant found in EFPC and its floodplain.

  17. Note: Wide band amplifier for quartz tuning fork sensors with digitally controlled stray capacitance compensation

    NASA Astrophysics Data System (ADS)

    Peng, Ping; Hao, Lifeng; Ding, Ning; Jiao, Weicheng; Wang, Qi; Zhang, Jian; Wang, Rongguo

    2015-11-01

    We presented a preamplifier design for quartz tuning fork (QTF) sensors in which the stray capacitance is digitally compensated. In this design, the manually controlled variable capacitor is replaced by a pair of varicap diodes, whose capacitance could be accurately tuned by a bias voltage. A tuning circuit including a single side low power operational amplifier, a digital-to-analog converter, and a microprocessor is also described, and the tuning process can be conveniently carried out on a personal computer. For the design, the noise level was investigated experimentally.

  18. Frequency Modulation Atomic Force Microscopy in Ionic Liquid Using Quartz Tuning Fork Sensors

    NASA Astrophysics Data System (ADS)

    Ichii, Takashi; Fujimura, Motohiko; Negami, Masahiro; Murase, Kuniaki; Sugimura, Hiroyuki

    2012-08-01

    Frequency modulation atomic force microscopy (FM-AFM) imaging in ionic liquids (ILs) were carried out. A quartz tuning fork sensor with a sharpened tungsten tip was used as a force sensor instead of a Si cantilever. Only the tip apex was immersed in ILs and the quality factor of the sensors was kept more than 100 in spite of the high viscosity of ILs. Atomic-resolution topographic imaging was successfully achieved in an IL as well as in an aqueous solution. In addition, frequency shift versus tip-to-sample distance curves were obtained and the structures of local solvation layers were studied.

  19. Ground-water aspects of the lower Henrys Fork region, Idaho

    USGS Publications Warehouse

    Crosthwaite, E.J.; Mundorff, M.J.; Walker, E.H.

    1967-01-01

    The lower Henrys Fork region includes the plains and low benches between Ashton and the junction of Henrys Fork and Snake River in eastern Idaho. The northwestern and western parts of the area are part of the Snake River lava plain. The central part of the area is occupied by alluvial plains of the Snake, Teton, and Falls Rivers, and Henrys Fork. The southeastern part of the area is a bench (Rexburg Bench), chiefly on silicic and basaltic volcanic rocks, which rises gradually to mountain peaks southeast of the area. The basalt, and the sand and gravel under the alluvial plains are good aquifers and yield large amounts of water with small drawdowns. The silicic volcanic rocks and the interbedded ash, pyroclastics, and sedimentary deposits generally yield much less water than the basalt and alluvium. The regional water table slopes southward beneath the basalt and alluvial plains. Seepage from stream channels and irrigated tracts has resulted in an extensive body of ground water perched above the regional water table. The perched water in part moves vertically down to the regional water table and in part laterally to the streams. Ground water beneath the Rexburg Bench moves generally northwestward to join the regional ground-water body beneath the alluvial plain. The regional water table is below the level of the streams in the area and ground water in the main aquifer, therefore is not tributary to the streams. Recharge to the regional water table is estimated to average 725,000 acre-feet annually. Pumping from the regional ground-water reservoir for irrigation or other uses would have no effect on streamflow or surface-water rights within the study area. However, depletion of the underflow would eventually reduce the inflow to American Falls Reservoir unless the depletion was offset by additional recharge. Total withdrawals of ground water for irrigation in 1962 were estimated to be 25,000 acre-feet and caused no significant decline in the water table. In the

  20. A homemade atomic force microscope based on a quartz tuning fork for undergraduate instruction

    NASA Astrophysics Data System (ADS)

    Li, Yingzi; Zhang, Liwen; Shan, Guanqiao; Song, Zihang; Yang, Rui; Li, Hua; Qian, Jianqiang

    2016-06-01

    Atomic force microscopes are a key tool in nanotechnology that overcome the limitations of optical microscopes and provide imaging capabilities with nanoscale resolution. We have developed an atomic force microscope that uses an inexpensive quartz tuning fork as a micro cantilever. Because of its ease of operation and its open structure, it can be easily customized by students. Due to its low costs, it is possible that every student in the course has access to one setup, allowing all students to obtain deep insights into nanotechnology and to understand the principles of atomic force microscopy.

  1. Thermoelastic investigation of a quartz tuning fork used in infrared spectroscopy

    SciTech Connect

    Spajer, M. Cavallier, B.; Euphrasie, S.; Matten, G.; Vacheret, X.; Vairac, P.; Vernier, D.; Jalocha, A.

    2013-11-11

    The performances of quartz tuning forks (QTF) used in infrared spectroscopy for pollutant detection are investigated. The transduction between light and QTF vibration is elucidated, thanks to QTF encapsulation under vacuum. From the sensitivity enhancement which is obtained, we conclude that their interaction is photo-thermoelastic rather than photo-thermoacoustic. A mapping of the local sensitivity of the QTF is obtained by scanning its faces with the excitation probe beam. The comparison between the signal mapping and the theoretical strain mapping indicates that the most efficient areas of the QTF correspond to the areas where the strain or stress is the highest.

  2. Photo-thermal quartz tuning fork excitation for dynamic mode atomic force microscope

    SciTech Connect

    Bontempi, Alexia; Teyssieux, Damien; Thiery, Laurent; Hermelin, Damien; Vairac, Pascal; Friedt, Jean-Michel

    2014-10-13

    A photo-thermal excitation of a Quartz Tuning Fork (QTF) for topographic studies is introduced. The non-invasive photo-thermal excitation presents practical advantages compared to QTF mechanical and electrical excitations, including the absence of the anti-resonance and its associated phase rotation. Comparison between our theoretical model and experiments validate that the optical transduction mechanism is a photo-thermal rather than photo-thermoacoustic phenomenon. Topographic maps in the context of near-field microscopy distance control have been achieved to demonstrate the performance of the system.

  3. Stalled fork rescue via dormant replication origins in unchallenged S phase promotes proper chromosome segregation and tumor suppression

    PubMed Central

    Kawabata, Tsuyoshi; Luebben, Spencer W.; Yamaguchi, Satoru; Ilves, Ivar; Matise, Ilze; Buske, Tavanna; Botchan, Michael R.; Shima, Naoko

    2011-01-01

    Summary Eukaryotic cells license far more origins than are actually used for DNA replication, thereby generating a large number of dormant origins. Accumulating evidence suggests that such origins play a role in chromosome stability and tumor suppression, though the underlying mechanism is largely unknown. Here, we show that a loss of dormant origins results in an increased number of stalled replication forks even in unchallenged S phase in primary mouse fibroblasts derived from embryos homozygous for the Mcm4Chaos3 allele. We found that this allele reduces the stability of the MCM2-7 complex, but confers normal helicase activity in vitro. Despite the activation of multiple fork recovery pathways, replication intermediates in these cells persist into M phase, increasing the number of abnormal anaphase cells with lagging chromosomes and/or acentric fragments. These findings suggest that dormant origins constitute a major pathway for stalled fork recovery, contributing to faithful chromosome segregation and tumor suppression. PMID:21362550

  4. The Influence of Humidity on the Shear Force Between Tip and Sample in Nsom Using Piezoelectric Fork

    NASA Astrophysics Data System (ADS)

    Zhang, Tianhao; Fang, Zheyu; Zheng, Jianya; Gao, Limo; Yang, Haidong; Yin, Meirong; Yang, Jia; Lu, Yanzhen; Kang, Huizhen; Yang, Dapeng; Yang, Huizhan

    The distance between tip and sample can be regulated using piezoelectric quartz fork glued with micro optic fiber probe. A biquadrate vibration equation for the fork-probe-sample system is established to theoretically analyze the relations of the electric current flow through fork versus tip-sample (T-S) distance (I-d). The I-d curve and the action distance for shear force are influenced by environmental humidity. The results reinforce the opinions in the earlier works by other researches that the physical origin of the shear force is due to a material filling the tip-sample gap. Furthermore, the intrinsic reasons for shear force damping between tip and sample have been confirmed and developed, i.e. the water and hydrocarbon coupled between optic fiber probe and sample due to the capillary cohesion force.

  5. Qualitative Assessment: Evaluating the Impacts of Climate Change on Endangered Species Act Recovery Actions for the South Fork Nooksack River, WA

    EPA Science Inventory

    The South Fork Nooksack River (South Fork) is located in northwest Washington State and is home to nine species of Pacific salmon, including Nooksack early Chinook (aka, spring Chinook salmon), an iconic species for the Nooksack Indian Tribe. The quantity of salmon in the South F...

  6. Stream-Sediment Geochemistry in Mining-Impacted Drainages of the Yankee Fork of the Salmon River, Custer County, Idaho

    USGS Publications Warehouse

    Frost, Thomas P.; Box, Stephen E.

    2009-01-01

    This reconnaissance study was undertaken at the request of the USDA Forest Service, Region 4, to assess the geochemistry, in particular the mercury and selenium contents, of mining-impacted sediments in the Yankee Fork of the Salmon River in Custer County Idaho. The Yankee Fork has been the site of hard-rock and placer mining, primarily for gold and silver, starting in the 1880s. Major dredge placer mining from the 1930s to 1950s in the Yankee Fork disturbed about a 10-kilometer reach. Mercury was commonly used in early hard-rock mining and placer operations for amalgamation and recovery of gold. During the late 1970s, feasibility studies were done on cyanide-heap leach recovery of gold from low-grade ores of the Sunbeam and related deposits. In the mid-1990s a major open-pit bulk-vat leach operation was started at the Grouse Creek Mine. This operation shut down when gold values proved to be lower than expected. Mercury in stream sediments in the Yankee Fork ranges from below 0.02 ppm to 7 ppm, with the highest values associated with old mill locations and lode and placer mines. Selenium ranges from below the detection limit for this study of 0.2 ppm to 4 ppm in Yankee Fork sediment samples. The generally elevated selenium content in the sediment samples reflect the generally high selenium contents in the volcanic rocks that underlie the Yankee Fork and the presence of gold and silver selenides in some of the veins that were exploited in the early phases of mining.

  7. Dynamics of a piezoelectric tuning fork/optical fiber assembly in a near-field scanning optical microscope

    NASA Astrophysics Data System (ADS)

    Shelimov, Konstantin B.; Davydov, Dmitri N.; Moskovits, Martin

    2000-02-01

    Factors leading to a decrease in the resonance quality (the Q factor) of quartz microtuning fork/optical fiber assemblies used as sensing elements in near-field scanning optical microscopes were considered using a simple elastomechanical analysis. Experiments to test the predictions of the analysis were carried out and strategies for recovering high Q factors were proposed and tested. Three major factors affecting the magnitude of the Q factor are discussed. The first is the stiffness imparted to the tine of the microtuning fork by the optical fiber attached to it; the second is the location of the attachment point of the fiber along the tine; the third is the resonant vibrational excitation of the fiber tip which acts as an energy dissipative channel. For tapping mode operation using a standard 125 μm diameter fiber, the large longitudinal stiffness of the fiber results in a dramatic Q-factor degradation. This effect can be overcome by reducing the diameter of the fiber cladding, d, and by slightly bending the fiber. Under these conditions, bending rather than longitudinal stretching dominates the fiber dynamics. The effective bending force constant for a thinned fiber is predicted to be proportional to d4. A sharp upturn in the Q factor is observed for d⩽25 μm, consistent with this prediction. The effective stiffness and mass of the fiber are also expected to scale approximately as x3, where x is the distance from the point of attachment of the fiber to the fork's base. Hence, the Q factor can be improved further by attaching the fiber closer to the tuning fork's base. Vibrational coupling between the tuning fork and the probe tip can result in a substantial Q-factor degradation for tips of a certain size. By taking these insights into consideration, we were able to construct tapping mode tuning fork/optical fiber assemblies with Q factors of up to 9000.

  8. Identifying sediment sources and quantifying rates of erosion along the North Fork Toutle River near Mount St. Helens, WA

    NASA Astrophysics Data System (ADS)

    Pitlick, J.; Meertens, C. M.; Major, J. J.; Normandeau, J.; Spicer, K.

    2010-12-01

    Traditional methods for measuring surface erosion are labor intensive and subject to large uncertainty due to the spatial variability in topography and stochastic nature of sediment transport processes. High-resolution Terrestrial Laser Scanning (TLS) measurements of surface topography have the potential to shorten the time involved in taking measurements as well as improve the accuracy of the surface change data. In this presentation we describe a pilot project that uses TLS to track erosion and deposition along the North Fork Toutle River (NFTR) near Mount St. Helens, WA. Since the May 1980 eruption of Mount St. Helens, the NFTR has been incising through the deposits of the massive debris avalanche generated at the start of the eruption. These deposits are still largely free of vegetation and present an ideal opportunity to examine erosional processes. In August, 2010, we performed initial surveys of two 0.5-km-long swaths of representative areas of the NFTR valley. Each of the two 250-300-meter diameter survey areas was scanned from multiple positions at cm-level spacing, and the resulting point cloud was georeferenced using co-located GPS. Measurements from this year's campaign will be used to develop a baseline digital elevation model (DEM) to track future changes in side-slope and valley-floor topography. Data from overlapping scans from this initial survey will be used to further evaluate the resolution of surface change measurements. In subsequent campaigns, the same areas will be mapped, and by differencing the scanned surfaces, we hope to identify prominent sediment sources and develop fine-scale quantitative estimates of localized erosion and deposition. In the long term we expect to use these surveys to test hypotheses regarding the relative contributions of sediment eroded from hillslopes versus sediment eroded from the valley floor.

  9. Spatial and Temporal influence of Redondo Peak headwaters in the East Fork Jemez River using Principal Component Analysis approach, Valles Caldera, New Mexico

    NASA Astrophysics Data System (ADS)

    Sanchez, Rodrigo; Meixner, Thomas

    2015-04-01

    The Valles Caldera is a volcanic collapse feature located in the Jemez Mountains in northern New Mexico, southwestern United States. This region is characterized by a bimodal precipitation pattern, i.e. spring snowmelt and summer monsoon rains. Two main streams flow through the Valles Caldera: San Antonio and East Fork Jemez. The junction of these two rivers form the Jemez River which is an important contributor to the Rio Grande that supplies water to cities located in southwestern U.S. Redondo Peak is located in the center of the Valles Caldera that has several springs that drain around all sides of the peak with different hydrologic responses. The main catchments (headwaters) identified in Redondo Peak are: La Jara, Upper Jaramillo, History Grove and Upper Redondo. The main questions that are going to be answered in this research are: Do these head waters affect the chemistry in East Fork Jemez river? and if so, how does this influence vary in space and time? A Principal Component Analysis (PCA) was performed using analyzed water samples for water isotopes and major anions and cations. These samples were collected from the flumes located at each catchment in Redondo Peak, and at different locations along the East Fork Jemez. Samples from the most consistent analytes from 2011 to 2013 were used in this present work. A first PCA was performed to assess how different the La Jara catchment is from Upper Redondo, Upper Jaramillo and History Grove based on the geochemistry of each basin. Prior the analysis the data needs to be normalized in order to avoid biasing towards extreme values. Since La Jara is the reference site for this analysis, its mean and standard deviation were used to normalized the data set of the other catchments. In order to simplify this analysis the two first principal components for each catchment were used to do the projections regardless how much of the variability can be explained. However future analyses are going to be performed with those

  10. Stalled replication fork repair and misrepair during thymineless death in Escherichia coli.

    PubMed

    Kuong, Kawai J; Kuzminov, Andrei

    2010-06-01

    Starvation for DNA precursor dTTP, known as 'thymineless death' (TLD), kills bacterial and eukaryotic cells alike. Despite numerous investigations, toxic mechanisms behind TLD remain unknown, although wrong nucleotide incorporation with subsequent excision dominates the explanations. We show that kinetics of TLD in Escherichia coli is not affected by mutations in DNA repair, ruling out excision after massive misincorporation as the cause of TLD. We found that the rate of DNA synthesis in thymine-starved cells decreases exponentially, indicating replication fork stalling. Processing of stalled replication forks by recombinational repair is known to fragment the chromosome, and we detect significant chromosomal fragmentation during TLD. Moreover, we report that, out of major recombinational repair functions, only inactivation of recF and recO relieves TLD, identifying the poisoning mechanism. Inactivation of recJ and rep has slight effect, while the recA, recBC, ruvABC, recG and uvrD mutations all accelerate TLD, identifying the protection mechanisms. Our epistatic analysis argues for two distinct pathways protecting against TLD: RecABCD/Ruv repairs the double-strand breaks, whereas UvrD counteracts RecAFO-catalyzed toxic single-strand gap processing. PMID:20465561

  11. Quartz Tuning Fork Pressure Gauge for High-Pressure Liquid Helium

    NASA Astrophysics Data System (ADS)

    Botimer, J.; Velasco, A.; Taborek, P.

    2016-08-01

    We have measured the quality factor Q and the frequency f of a 32-kHz quartz tuning fork immersed in liquid ^4 He between 0.9 and 3.0 K, over pressures ranging from the saturated vapor pressure to ≈ 25 atm. At constant pressure, as a function of temperature, the quality factor and frequency have strong features related to the temperature dependence of the superfluid fraction. At constant temperature, Q depends on the superfluid fraction, while the frequency is a smooth function of pressure. The behavior is explained using a simple hydrodynamic model. The liquid helium viscosity is obtained from measured values of Q, and together with tabulated values of the helium density as a function of pressure and temperature, the frequency shift can be parameterized as a function of temperature and pressure. The observed sensitivity is ≈ 7.8 Hz/atm. The quartz tuning fork provides a compact low power method of measuring the pressure in the bulk liquid.

  12. Fresnel and Fraunhofer diffraction of a Gaussian laser beam by fork-shaped gratings.

    PubMed

    Janicijevic, Ljiljana; Topuzoski, Suzana

    2008-11-01

    Expressions describing the vortex beams that are generated by the process of Fresnel diffraction of a Gaussian beam incident out of waist on fork-shaped gratings of arbitrary integer charge p, and vortex spots in the case of Fraunhofer diffraction by these gratings, are deduced. The common general transmission function of the gratings is defined and specialized for the cases of amplitude holograms, binary amplitude gratings, and their phase versions. Optical vortex beams, or carriers of phase singularity with charges mp and -mp, are the higher negative and positive diffraction-order beams. The radial part of their wave amplitudes is described by the product of the mpth-order Gauss-doughnut function and a Kummer function, or by the first-order Gauss-doughnut function and the difference of two modified Bessel functions whose orders do not match the singularity charge value. The wave amplitude and the intensity distributions are discussed for the near and far fields in the focal plane of a convergent lens, as well as the specialization of the results when the grating charge p=0; i.e., the grating turns from forked into rectilinear. The analytical expressions for the vortex radii are also discussed. PMID:18978843

  13. Lentiform fork sign: a magnetic resonance finding in a case of acute metabolic acidosis.

    PubMed

    Grasso, Daniela; Borreggine, Carmela; Perfetto, Francesco; Bertozzi, Vincenzo; Trivisano, Marina; Specchio, Luigi Maria; Grilli, Gianpaolo; Macarini, Luca

    2014-06-01

    We report a 33 year-old woman addicted to chronic unspecified solvents abuse with stupor, respiratory disorders, tetraplegia and severe metabolic acidosis. On admission an unenhanced cranial CT scan showed symmetrical hypodensities of both lentiform nuclei. MR imaging performed 12 hours after stupor demonstrates bilateral putaminal hemorrhagic necrosis, bilateral external capsule, corona radiata and deep cerebellar hyperintensities with right cingulate cortex involvement. DWI reflected bilateral putaminal hyperintensities with restricted water diffusion as to citotoxic edema and development of vasogenic edema in the external capsule recalling a fork. On day twenty, after specific treatments MRI demonstrated a bilateral putaminal marginal enhancement. Bilateral putaminal necrosis is a characteristic but non-specific radiological finding of methanol poisoning. Lentiform Fork sign is a rare MRI finding reported in literature in 22 patients with various conditions characterized by metabolic acidosis. Vasogenic edema may be due to the differences in metabolic vulnerability between neurons and astrocytes. We postulate that metabolic acidosis could have an important role to generate this sign. PMID:24976195

  14. Mussels: the forgotten fauna of regulated rivers: a case study of the Caney Fork River

    USGS Publications Warehouse

    Layzer, James B.; Gordon, Mark E.; Anderson, Robert M.

    1993-01-01

    During the past century freshwater mussel populations have declined precipitously throughout North America. Much of this loss has resulted from the construction of dams. In the Cumberland River system, 23% (22 species) of the historic mussel fauna is extinct or listed as endangered. Several additional species have either been extirpated from the Cumberland River or exist only in small, non-reproducing populations. Mussels of headwater streams have been severely affected by coal mining and poor land use practices. An intensive survey was conducted in the Caney Fork River, a major tributary to the Cumberland River, to determine the historic and extant mussel fauna. The results indicate that at least 37 species of mussels have been extirpated from the Caney Fork River, mainly as a result of the construction and operation of the Center Hill Dam. Among the species extirpated, two are now extinct, five are endangered and five are candidates for listing as threatened or endangered. Effects associated with this dam include the inundation of 102 km of riverine habitat, the discharge of hypolimnetic water (which limits mussel reproduction) and an alternating pattern of stream bed scouring and dewatering. The recognition of mussel life history requirements during preconstruction could have reduced many of these effects.

  15. Lentiform Fork Sign: a Magnetic Resonance Finding in a Case of Acute Metabolic Acidosis

    PubMed Central

    Grasso, Daniela; Borreggine, Carmela; Perfetto, Francesco; Bertozzi, Vincenzo; Trivisano, Marina; Specchio, Luigi Maria; Grilli, Gianpaolo; Macarini, Luca

    2014-01-01

    Summary We report a 33 year-old woman addicted to chronic unspecified solvents abuse with stupor, respiratory disorders, tetraplegia and severe metabolic acidosis. On admission an unenhanced cranial CT scan showed symmetrical hypodensities of both lentiform nuclei. MR imaging performed 12 hours after stupor demonstrates bilateral putaminal hemorrhagic necrosis, bilateral external capsule, corona radiata and deep cerebellar hyperintensities with right cingulate cortex involvement. DWI reflected bilateral putaminal hyperintensities with restricted water diffusion as to citotoxic edema and development of vasogenic edema in the external capsule recalling a fork. On day twenty, after specific treatments MRI demonstrated a bilateral putaminal marginal enhancement. Bilateral putaminal necrosis is a characteristic but non-specific radiological finding of methanol poisoning. Lentiform Fork sign is a rare MRI finding reported in literature in 22 patients with various conditions characterized by metabolic acidosis. Vasogenic edema may be due to the differences in metabolic vulnerability between neurons and astrocytes. We postulate that metabolic acidosis could have an important role to generate this sign. PMID:24976195

  16. The Oak Ridge Y-12 Plant biological monitoring and abatement program for East Fork Poplar Creek

    SciTech Connect

    Loar, J.M.; Adams, S.M.; Allison, L.J.; Giddings, J.M.; McCarthy, J.F.; Southworth, G.R.; Smith, J.G.; Stewart, A.J.; Springborn Bionomics, Inc., Wareham, MA; Oak Ridge National Lab., TN )

    1989-10-01

    In May 1985, a National Pollutant Discharge Elimination System permit was issued for the Oak Ridge Y-12 Plant, a nuclear weapons components production facility located in Oak Ridge, Tennessee, and operated by Martin Marietta Energy Systems, Inc., for the US Department of Energy. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Oak Ridge Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek), in particular, the growth and propagation of fish and aquatic life, as designated by the Tennessee Department of Health and Environment. A second purpose for the BMAP is to document the ecological effects resulting from implementation of a water pollution control program that will include construction of nine new wastewater treatment facilities over the next 4 years. Because of the complex nature of the effluent discharged to East Fork Poplar Creek and the temporal and spatial variability in the composition of the effluent (i.e., temporal variability related to various pollution abatement measures that will be implemented over the next several years and spatial variability caused by pollutant inputs downstream of the Oak Ridge Y-12 Plant), a comprehensive, integrated approach to biological monitoring was developed for the BMAP. 39 refs., 5 figs., 8 tabs.

  17. Resonance frequency-retuned quartz tuning fork as a force sensor for noncontact atomic force microscopy

    SciTech Connect

    Ooe, Hiroaki; Sakuishi, Tatsuya; Arai, Toyoko; Nogami, Makoto; Tomitori, Masahiko

    2014-07-28

    Based on a two-prong type quartz tuning fork, a force sensor with a high Q factor, which we call a retuned fork sensor, was developed for non-contact atomic force microscopy (nc-AFM) with atomic resolution. By cutting a small notch and attaching an AFM tip to one prong, its resonance frequency can be retuned to that of the other intact prong. In balancing the two prongs in this manner, a high Q factor (>50 000 in ultrahigh vacuum) is obtained for the sensor. An atomic resolution image of the Si(111)-7 × 7 surface was demonstrated using an nc-AFM with the sensor. The dependence of the Q factor on resonance frequency of the sensor and the long-range force between tip and sample were measured and analyzed in view of the various dissipation channels. Dissipation in the signal detection circuit turned out to be mainly limited by the total Q factor of the nc-AFM system.

  18. Mode locking effects on the playing frequency for fork fingerings on the clarinet.

    PubMed

    Nederveen, C J; Dalmont, J-P

    2012-01-01

    The non-linear excitation of wind instruments generates higher harmonics of the playing frequency. These higher harmonics are coupled to resonances in the pipe. This is called mode locking. When the pipe modes are not harmonic, the playing frequency shifts away from the fundamental in order to maximize the output. It may go up or down, depending on the position of the modes and the amplitude. The effect is especially manifest for fork fingerings. Three fork fingerings on a clarinet were investigated. They were artificially blown between the threshold and extinction pressure. A time domain simulation was carried out based on a lumped model of the excitation coupled to an input impedance calculated from the instrument dimensions. At low amplitudes the fundamental frequency dominates and the playing frequency is governed by the position of the first peak in the input impedance spectra. At higher blowing pressures the playing frequency shifts. For both blowing and simulation this follows the same pattern. The frequencies predicted by the calculations are higher than the values found by blowing, which may be due to inadequacies in the model description, to uncertainties of the various parameters, as reed stiffness, moving reed area, and the properties of the slit flow. PMID:22280690

  19. Middle Fork Holston River watershed biological assessment, summers of 1986 and 1987

    SciTech Connect

    Saylor, C.F.; Hill, D.M.; Ahlstedt, S.A.; Brown, A.M.

    1988-05-01

    Fish and macroinvertebrates were collected to obtain a qualitative and quantitative determination relating the impacts of land use activities on aquatic fauna and water quality in watersheds of the Middle Fork Holston River. Samples were compared to historical data, used for calculating an Index of Biological Integrity (IBI), and examined for diversity and abundance of macroinvertebrates. Most IBI's determined for subwatersheds of the Middle Fork Holston River (MFHR) supported land use inventories and soil loss estimates previously completed by TVA in 1985. These inventories targeted streams likely to be impacted by siltation, agricultural runoff, and other nonpoint pollution sources. Findings indicated that siltation and enrichment had increased in the mainstream of MFHR since 1969. Several sites exhibited characteristics implying additional forms of pollution which were impacting the fauna. Benthic macroinvertebrate samples were helpful in documenting degradation but did not always support IBI's and land use inventory and soil loss estimates. Macroinvertebrate fauna are typically more resilient and recover faster than fish fauna, offering a partial explanation for this phenomenon. 6 refs., 3 figs., 12 tabs.

  20. Dynamic behavior of tuning fork shear-force structures in a SNOM system.

    PubMed

    Gao, Fengli; Li, Xide; Wang, Jia; Fu, Yu

    2014-07-01

    Piezoelectric tuning fork shear-force structures are widely used as a distance control unit in a scanning near-field optical microscopy. However, the complex dynamic behavior among the micro-tuning forks (TFs), optical fiber probes, and the probe-surface interactions is still a crucial issue to achieve high-resolution imaging or near-field interaction inspections. Based on nonlinear beam tension-bending vibration theory, vibration equations in both longitudinal and lateral directions have been established when the TF structure and the optical fiber are treated as deformable structures. The relationship of the probe-surface interaction induced by Van der Waals force has been analyzed and the corresponding numerical results used to describe the vibrational behavior of the probe approaching the sample surface are obtained. Meanwhile, the viscous resistance of the liquid film on the sample surface has also been investigated using linear beam-bending vibration theory. Experiments testing the interaction between the probe and the water film on a single crystal silicon wafer have been carried out and the viscous resistance of the water film was estimated using the established equations. Finally, to use the TF-probe structure as a force sensor, the relation between the dynamic response of the TF-probe system and an external force on the probe tip was obtained. PMID:24815548