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Sample records for fr e1 transition

  1. Octupole deformation in sup 221 Fr; E1 transition rates

    SciTech Connect

    Liang, C.F.; Peghaire, A. ); Sheline, R.K. )

    1990-07-10

    Experimental data following the alpha decay of{sup 225}Ac are interpreted in terms of a spectroscopy in {sup 221}Fr consistent with octupole deformation. However, the measured E1 transition probabilities suggest that the low lying bands in {sup 221}Fr are considerably more mixed than in nuclei with slightly higher mass number. It is suggested that this mixing of states in {sup 221}Fr is indicative of the partial collapse of Nilsson-like orbitals into more degenerate shell model orbitals.

  2. E1-forbidden transition rates in ions of astrophysical interest

    NASA Astrophysics Data System (ADS)

    Träbert, E.

    2014-11-01

    Transition rates in atomic systems may appear to be of little importance in steady-state plasmas that are observed at great distances from Earth. However, some of the transition rates compete with collision rates, and in these cases certain line intensity ratios are affected and can serve as remote indicators of density. In the low-density environments of stellar coronae and planetary nebulae, the transition rates of interest are mostly spin-forbidden E1 decays, higher-multipole order transitions (M1, E2, M2, M3), and hyperfine-induced transitions. On Earth, measurements of the long upper level lifetimes of these atomic systems require the use of ion traps. A fair number of test cases with lifetimes in the range from nanoseconds to many seconds have been treated successfully, and the evolution of calculations along with the experimental progress is notable. A new generation of cold ion traps is expected to extend the atomic lifetime measurements on multiply charged ions into the range of many minutes.

  3. Rates of E1, E2, M1, and M2 transitions in Ni II

    NASA Astrophysics Data System (ADS)

    Cassidy, C. M.; Hibbert, A.; Ramsbottom, C. A.

    2016-03-01

    Aims: We present rates for all E1, E2, M1, and M2 transitions among the 295 fine-structure levels of the configurations 3d9, 3d84s, 3d74s2, 3d84p, and 3d74s4p, determined through an extensive configuration interaction calculation. Methods: The CIV3 code developed by Hibbert and coworkers is used to determine for these levels configuration interaction wave functions with relativistic effects introduced through the Breit-Pauli approximation. Results: Two different sets of calculations have been undertaken with different 3d and 4d functions to ascertain the effect of such variation. The main body of the text includes a representative selection of data, chosen so that key points can be discussed. Some analysis to assess the accuracy of the present data has been undertaken, including comparison with earlier calculations and the more limited range of experimental determinations. The full set of transition data is given in the supplementary material as it is very extensive. Conclusions: We believe that the present transition data are the best currently available. Full Table 4 and Tables 5-8 are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/587/A107

  4. Calculation of energy levels, {ital E}1 transition amplitudes, and parity violation in francium

    SciTech Connect

    Dzuba, V.A.; Flambaum, V.V.; Sushkov, O.P.

    1995-05-01

    Many-body perturbation theory in the screened Coulomb interaction was used to calculate energy levels, {ital E}1 trransition amplitudes, and the parity-nonconserving (PNC) {ital E}1 amplitude of the 7{ital s}-8{ital s} transition in francium. The method takes into account the core-polarization effect, the second-order correlations, and the three dominating sequences of higher-order correlation diagrams: screening of the electron-electron interaction, particle-hole interaction, and the iterations of the self-energy operator. The result for the PNC amplitude for {sup 223}Fr is {ital E}1(7{ital s}-8{ital s})=(1.59{plus_minus}{similar_to}1%){times}10{sup {minus}10}{ital iea}{sub {ital B}}({minus}{ital Q}{sub {ital W}}/{ital N}), where {ital Q}{sub {ital W}} is the weak charge of the nucleus, {ital N}=136 is the number of neutrons, {ital e}={vert_bar}{ital e}{vert_bar} is the elementary charge, and {ital a}{sub {ital B}} is the Bohr radius. Our prediction for the position of the 8{ital s} energy level of Fr, which has not been measured yet, is 13 110 cm{sup {minus}1} below the limit of the continuous spectrum. The accuracy of the calculations was controlled by comparison with available experimental data and analogous calculations for cesium. It is estimated to be {similar_to}0.1% for the energy levels and {similar_to}1% for the transition amplitudes.

  5. Enhanced E1 transitions and {alpha}-clustering in {sup 212}Po

    SciTech Connect

    Suzuki, Y.; Ohkubo, S.

    2011-05-06

    An {alpha}+{sup 208}Pb(0{sup +},3{sup -}) cluster model explains the recently observed enhanced E1 transitions from the new negative-parity levels to the yrast states in {sup 212}Po. Heavy and light nuclei present good examples of surface clustering and well-localized clustering.

  6. Collective vector method for calculation of E1 moments in atomic transition arrays

    SciTech Connect

    Bloom, S.D.; Goldberg, A.

    1985-10-01

    The CV (collective vector) method for calculating E1 moments for a transition array is described and applied in two cases, herein denoted Z26A and Z26B, pertaining to two different configurations of iron VI. The basic idea of the method is to create a CV from each of the parent (''initial state'') state-vectors of the transition array by application of the E1 operator. The moments of each of these CV's, referred to the parent energy, are then the rigorous moments for that parent, requiring no state decomposition of the manifold of daughter state-vectors. Since, in cases of practical interest, the daughter manifold can be orders of magnitude larger in size than the parent manifold, this makes possible the calculation of many moments higher than the second in situations hitherto unattainable via standard methods. The combination of the moments of all the parents, with proper statistical weighting, then yields the transition array moments from which the transition strength distribution can be derived by various procedures. We describe two of these procedures: (1) The well-known GC (Gram-Charlier) expansion in terms of Hermite polynomials, (2) The Lanczos algorithm or Stieltjes imaging method, also called herein the delta expansion. Application is made in the cases of Z26A (50 lines) and Z26B (5523 lines) and the relative merits and shortcomings of the two procedures are discussed. 10 refs., 15 figs., 2 tabs.

  7. E1, E2 and M1 transition parameters for some levels over ionization limit of Ne III

    NASA Astrophysics Data System (ADS)

    Eser, Selda; Özdemir, Leyla

    2016-07-01

    We have reported the level energies and radiative transition ( E1 , E2 and M1 parameters, such as wavelengths, transition rates, oscillator strengths and line strengths for some levels over the ionization limit of Ne III (oxygen-like). The calculations have been performed using the general-purpose relativistic atomic structure package (GRASP) based on the fully relativistic multiconfiguration Dirac-Fock (MCDF) method. The results obtained have been compared with the available theoretical and experimental values in the literature.

  8. E1 and E2 transitions for Fe XVI, Co XVII and Ni XVIII

    NASA Astrophysics Data System (ADS)

    Çelik, G.; Ateş, Ş.

    2016-07-01

    Electric dipole and electric quadrupole transition data for sodium-like iron, cobalt and nickel have been calculated within the weakest bound electron potential model (WBEPM) theory using experimental energy levels and theoretical expectation values of orbital radii corresponding to those energy levels under the assumption of the {LS} coupling scheme. The results obtained from this study provide theoretical transition probability and oscillator strength data requested in many fields of researches, especially astrophysics. The calculated transition data results have been compared with available data in the literature. The present results are consistent with earlier calculations. Some new electric quadrupole transition probability values not existing in the data bases, especially for iron have been obtained using this method.

  9. Enhanced E1 transitions and {alpha}+{sup 208}Pb(3{sup -}) clustering in {sup 212}Po

    SciTech Connect

    Suzuki, Y.; Ohkubo, S.

    2010-10-15

    We formulate a model for {sup 212}Po, based on the coupled-channels of {alpha}+{sup 208}Pb(0{sup +}) and {alpha}+{sup 208}Pb(3{sup -}) in which the {alpha}-Pb interaction contains scalar, quadrupole, and octupole terms. The model reproduces the recently observed enhanced E1 transitions from the several new negative-parity levels to the yrast states. Because these data are hard to understand in the shell model, this success gives a strong support for a unique role of {alpha}+{sup 208}Pb(3{sup -}) clustering in {sup 212}Po.

  10. A shock-tube measurement of the SiO/E 1 Sigma + - X 1 Sigma +/ transition moment

    NASA Technical Reports Server (NTRS)

    Park, C.

    1978-01-01

    The sum of the squares of the electronic transition moments for the (E 1 Sigma +) - (X 1 Sigma +) band system of SiO has been determined from absorption measurements conducted in the reflected-shock region of a shock tube. The test gas produced by shock-heating a mixture of SiCl4, N2O, and Ar, and the spectra were recorded photographically in the 150-230-nm wavelength range. The values of the sum of the squares were determined by comparing the measured absorption spectra with those produced by a line-by-line synthetic spectrum calculation. The value so deduced at an r-centroid value of 3.0 bohr was 0.86 + or - 0.10 atomic unit.

  11. 78 FR 15597 - Special Conditions: GE Aviation CT7-2E1 Turboshaft Engine Model

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... published on July 20, 2012 (77 FR 42677). We received six comments from European Aviation Safety Agency... the special conditions 33-002-SC, published on May 28, 1999 (64 FR 28900). We are therefore adopting...)(7), and 33.88(c). The 2.5 minute time duration for the rating would affect the structural...

  12. Energy levels, wavelengths, and transition rates of multipole transitions (E1, E2, M1, M2) in Au{sup 67+} and Au{sup 66+} ions

    SciTech Connect

    Hamasha, Safeia

    2013-11-15

    The fully relativistic configuration interaction method of the FAC code is used to calculate atomic data for multipole transitions in Mg-like Au (Au{sup 67+}) and Al-like Au (Au{sup 66+}) ions. Generated atomic data are important in the modeling of M-shell spectra for heavy Au ions and Au plasma diagnostics. Energy levels, oscillator strengths and transition rates are calculated for electric-dipole (E1), electric quadrupole (E2), magnetic dipole (M1), and magnetic quadrupole (M2) for transitions between excited and ground states 3l−nl{sup ′}, such that n=4,5,6,7. The local central potential is derived using the Dirac–Fock–Slater method. Correlation effects to all orders are considered by the configuration interaction expansion. All relativistic effects are included in the calculations. Calculated energy levels are compared against published values that were calculated using the multi-reference many body perturbation theory, which includes higher order QED effects. Favorable agreement was observed, with less than 0.15% difference.

  13. 77 FR 42677 - Special Conditions: General Electric CT7-2E1 Turboshaft Engine

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... Privacy Act Statement can be found in the Federal Register published on April 11, 2000 (65 FR 19477-19478... structural and operational characteristics that are time dependent, such as the values for transients, time duration for stabilization to steady state, and part growth due to deformation. In addition, we...

  14. On the enhanced E1 transitions in the K = 3/2 parity doublet band in 223Ra

    NASA Astrophysics Data System (ADS)

    Mach, H.; Lindroth, A.; Ruchowska, E.; Kvasil, J.; Fogelberg, B.; Gulda, K.; Aas, A. J.; Borge, M. J. G.; Grant, I. S.; Hagebø, E.; Kurcewicz, W.; Martinez, T.; Rubio, B.; Smith, J. F.; Steffensen, K.; Tain, J. L.; Tengblad, O.; Thorsteinsen, T. F.

    2016-06-01

    We have applied the fast timing βγγ (t) technique to remeasure lifetimes of selected states in 223Ra populated in the β- decay of 223Fr. T_{1/2}=587(12) ps and 210(13)ps have been obtained for the 3/2- and 5/2- states at 50.1 and 79.7 keV, that are more accurate than the previous values of 630(70)ps and 166(55)ps, respectively. Our \\vert D0\\vert value of 0.155(10)e·fm obtained for the K=3/2 configuration together with the available values of \\vert D0\\vert for the K=1/2 and K=5/2 parity doublet bands establish the configuration dependence of \\vert D0\\vert at low spins in this nucleus. Results of theoretical calculations performed for 223Ra, using the quasiparticle-phonon model (QPM) with inclusion of the Coriolis coupling, reasonably well reproduce octupole correlations in this nucleus.

  15. Radiative rates for E1, E2, M1, and M2 transitions in F-like ions with 37 ≤ Z ≤ 53

    NASA Astrophysics Data System (ADS)

    Aggarwal, Kanti M.; Keenan, Francis P.

    2016-05-01

    Calculations of energy levels, radiative rates and lifetimes are reported for 17 F-like ions with 37≤Z≤53. For brevity, results are only presented among the lowest 113 levels of the 2s22p5, 2s2p6, 2s22p43 ℓ, 2s2p53 ℓ, and 2p63 ℓ configurations, although the calculations have been performed for up to 501 levels in each ion. The general-purpose relativistic atomic structure package (GRASP) has been adopted for the calculations, and radiative rates (along with oscillator strengths and line strengths) are listed for all E1, E2, M1, and M2 transitions of the ions. Comparisons are made with earlier available experimental and theoretical energies, although these are limited to only a few levels for most ions. Therefore for additional accuracy assessments, particularly for energy levels, analogous calculations have been performed with the Flexible Atomic Code (FAC), for up to 72 259 levels. Limited previous results are available for radiative rates for comparison purposes, and no large discrepancy is observed for any transition and/or ion.

  16. Radiative rates for E1, E2, M1, and M2 transitions in Br-like ions with 43 ≤ Z ≤ 50

    NASA Astrophysics Data System (ADS)

    Aggarwal, Kanti M.; Keenan, Francis P.

    2016-01-01

    Energies and lifetimes are reported for the eight Br-like ions with 43 ≤ Z ≤ 50, namely Tc IX, Ru X, Rh XI, Pd XII, Ag XIII, Cd XIV, In XV, and Sn XVI. Results are listed for the lowest 375 levels, which mostly belong to the 4s24p5, 4s24p44ℓ, 4s4p6,4s24p45ℓ, 4s24p34d2, 4s4p54ℓ, and 4s4p55ℓ configurations. Extensive configuration interaction among 39 configurations (generating 3990 levels) has been considered and the general-purpose relativistic atomic structure package (GRASP) has been adopted for the calculations. Radiative rates are listed for all E1, E2, M1, and M2 transitions involving the lowest 375 levels. Previous experimental and theoretical energies are available for only a few levels of three, namely Ru X, Rh XI and Pd XII. Differences with the measured energies are up to 4% but the present results are an improvement (by up to 0.3 Ryd) in comparison to other recently reported theoretical data. Similarly for radiative rates and lifetimes, prior results are limited to those involving only 31 levels of the 4s24p5, 4s24p44d, and 4s4p6 configurations for the last four ions. Moreover, there are generally no discrepancies with our results, although the larger calculations reported here differ by up to two orders of magnitude for a few transitions.

  17. 77 FR 48733 - Transitional Program for Covered Business Method Patents-Definitions of Covered Business Method...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-14

    ... Covered Business Method Patents, 77 FR 7080 (Feb. 10, 2012), to provide rules specific to the transitional... Method Patents, 77 FR 7080 (Feb. 10, 2012) and Transitional Program for Covered Business Method Patents... Covered Business Method Patents, 77 FR 7080 (Feb. 10, 2012) (notice of proposed rulemaking)...

  18. 75 FR 43197 - Public Housing Assessment System (PHAS): Asset Management Transition Year 2 Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... notice, Public Housing Assessment System (PHAS): Asset Management Transition Year 2 Information (75 FR... Register notice (FR-5227- N-01), ``Public Housing Assessment System (PHAS): Asset Management Transition Year Information and Uniform Financial Reporting Standards (UFRS) Information'' (73 FR 49588). In...

  19. The E1 proteins

    SciTech Connect

    Bergvall, Monika; Melendy, Thomas; Archambault, Jacques

    2013-10-15

    E1, an ATP-dependent DNA helicase, is the only enzyme encoded by papillomaviruses (PVs). It is essential for replication and amplification of the viral episome in the nucleus of infected cells. To do so, E1 assembles into a double-hexamer at the viral origin, unwinds DNA at the origin and ahead of the replication fork and interacts with cellular DNA replication factors. Biochemical and structural studies have revealed the assembly pathway of E1 at the origin and how the enzyme unwinds DNA using a spiral escalator mechanism. E1 is tightly regulated in vivo, in particular by post-translational modifications that restrict its accumulation in the nucleus. Here we review how different functional domains of E1 orchestrate viral DNA replication, with an emphasis on their interactions with substrate DNA, host DNA replication factors and modifying enzymes. These studies have made E1 one of the best characterized helicases and provided unique insights on how PVs usurp different host-cell machineries to replicate and amplify their genome in a tightly controlled manner. - Highlights: • The papillomavirus E1 helicase orchestrates replication of the viral DNA genome. • E1 assembles into a double-hexamer at the viral origin with the help of E2. • E1 interacts with cellular DNA replication factors. • E1 unwinds DNA using a spiral escalator mechanism. • Nuclear accumulation of E1 is regulated by post-translational modifications.

  20. 78 FR 30951 - SBIR/STTR Phase I to Phase II Transition Benchmarks

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ... INFORMATION: Section 4(a)(3)(iii) of the SBIR Policy Directive (77 FR 46806) and the STTR Policy Directive (77 FR 46855) require each agency to establish an SBA-approved Phase I-Phase II Transition Rate benchmark... benchmarks can take effect. As a result, on October 16, 2012, at 77 FR 63410, SBA published the...

  1. 77 FR 35199 - Swap Data Recordkeeping and Reporting Requirements: Pre-Enactment and Transition Swaps

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-12

    ... Reporting Pre-Enactment Swap Transactions (``Pre-Enactment Swaps IFR''), 75 FR 63080 (Oct. 14, 2010...-Enactment Swap Transactions (``Post-Enactment Swaps IFR'' or ``Transition Swaps IFR''), 75 FR 78892 (Dec. 17... to particular swaps. \\19\\ 77 FR 2136 (February 13, 2012). With respect to recordkeeping, part...

  2. Multipole (E1, M1, E2, M2, E3, M3) transition wavelengths and rates between 3l-15l' excited and ground states in nickel-like ions

    SciTech Connect

    Safronova, U I; Safronova, A S; Beiersdorfer, P

    2006-05-04

    A relativistic many-body method is developed to calculate energy and transition rates for multipole transitions in many-electron ions. This method is based on relativistic many-body perturbation theory (RMBPT), agrees with MCDF calculations in lowest-order, includes all second-order correlation corrections and includes corrections from negative energy states. Reduced matrix elements, oscillator strengths, and transition rates are calculated for electric-multipole (dipole (E1), quadrupole (E2), and octupole (E3)) and magnetic-multipole (dipole (M1), quadrupole (M2), and octupole (M3)) transitions between 3l{sup -1}5l{prime} excited and ground states in Ni-like ions with nuclear charges ranging from Z = 30 to 100. The calculations start from a 1s{sup 2}s{sup 2}2p{sup 6}3s{sup 2}3p{sup 6}3d{sup 10} Dirac-Fock potential. First-order perturbation theory is used to obtain intermediate-coupling coefficients, and second-order RMBPT is used to determine the matrix elements. A detailed discussion of the various contributions to the dipole matrix elements and energy levels is given for nickel-like tungsten (Z = 74). The contributions from negative-energy states are included in the second order E1, M1, E2, M2, E3 and M3 matrix elements. The resulting transition energies and transition rates are compared with experimental values and with results from other recent calculations. These atomic data are important in modeling of M-shell radiation spectra of heavy ions generated in electron beam ion trap experiments and in M-shell diagnostics of plasmas.

  3. THE E1 PROTEINS

    PubMed Central

    Bergvall, Monika; Melendy, Thomas; Archambault, Jacques

    2013-01-01

    E1, an ATP-dependent DNA helicase, is the only enzyme encoded by papillomaviruses (PVs). It is essential for replication and amplification of the viral episome in the nucleus of infected cells. To do so, E1 assembles into a double-hexamer at the viral origin, unwinds DNA at the origin and ahead of the replication fork and interacts with cellular DNA replication factors. Biochemical and structural studies have revealed the assembly pathway of E1 at the origin and how the enzyme unwinds DNA using a spiral escalator mechanism. E1 is tightly regulated in vivo, in particular by post-translational modifications that restrict its accumulation in the nucleus. Here we review how different functional domains of E1 orchestrate viral DNA replication, with an emphasis on their interactions with substrate DNA, host DNA replication factors and modifying enzymes. These studies have made E1 one of the best characterized helicases and provided unique insights on how PVs usurp different host-cell machineries to replicate and amplify their genome in a tightly controlled manner. PMID:24029589

  4. A Large-scale Relativistic Configuration-interaction Approach: Application to the 4s2 - 4s4p Transition Energies and E1 Rates for Zn-like Ions

    SciTech Connect

    Chen, M H; Cheng, K T

    2009-08-28

    Relativistic configuration-interaction calculations of the 4s4p excitation energies and 4s{sup 2} - 4s4p E1 transitions for Zn-like ions from Z = 30 to 92 are shown. B-spline basis functions are used for these large-scale calculations. QED corrections to the excitation energies are also calculated. Results are in good agreement with other theories and with experiment, and demonstrate the utility of this method for high-precision atomic structure calculations not just for few-electron systems but also for large atomic systems such as Zn-like ions along the entire isoelectronic sequence.

  5. Translational regulation of Inhibin βA by TGFβ via the RNA-binding protein hnRNP E1 enhances the invasiveness of epithelial-to-mesenchymal transitioned cells

    PubMed Central

    Howley, Breege V; Hussey, George S; Link, Laura A; Howe, Philip H

    2015-01-01

    The epithelial-to-mesenchymal transition (EMT) is a cellular process that functions during embryonic development and tissue regeneration, thought to be aberrantly activated in epithelial-derived cancer and play an important role in the process of metastasis. The TGFβ signaling pathway is a key inducer of EMT and we have elucidated a post-transcriptional mechanism by which TGFβ modulates expression of select transcripts via the RNA binding protein hnRNP E1 during EMT. One such transcript inhibin βA is a member of the TGFβ superfamily. Here, we show by polysome profiling that inhibin βA is translationally regulated by TGFβ via hnRNP E1. TGFβ treatment or knockdown of hnRNP E1 relieves silencing of the inhibin βA transcript, resulting in increased protein expression and secreted levels of the inhibin βA homodimer, activin A. Our data indicates that the translational up-regulation of inhibin βA enhances the migration and invasion of cells that have undergone an EMT and promotes cancer progression in vivo. PMID:26096938

  6. 77 FR 63410 - SBIR/STTR Phase I to Phase II Transition Benchmarks

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ... 4(a)(3)(iii) of the SBIR Policy Directive, which was published on August 6, 2012, at 77 FR 46806 and the STTR Policy Directive, which was published the same day at 77 FR 46855, requires each agency to... ADMINISTRATION SBIR/STTR Phase I to Phase II Transition Benchmarks AGENCY: U.S. Small Business...

  7. 77 FR 7095 - Transitional Program for Covered Business Method Patents-Definition of Technological Invention

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    2012-02-10

    ... Flexibility Analysis for Patent-Related Regulations, 71 FR 67109 (Nov. 20, 2006), 1313 Off. Gaz. Pat. Office..., 71 FR at 67112 (Nov 20, 2006), 1313 Off. Gaz. Pat. Office at 63 (Dec. 12, 2006). As discussed above... Patent and Trademark Office 37 CFR Part 42 RIN 0651-AC75 Transitional Program for Covered Business...

  8. 76 FR 30997 - National Transit Database: Amendments to Urbanized Area Annual Reporting Manual

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... Federal Register (73 FR 7361) inviting comments on proposed amendments to the 2011 Annual Manual. This... Federal Register (75 FR 192) inviting comments on proposed amendments to the 2011 Annual Manual. FTA... Manual AGENCY: Federal Transit Administration (FTA), DOT. ACTION: Notice of Amendments to 2011...

  9. Radiative rates for E1, E2, M1, and M2 transitions in S-like to F-like tungsten ions (W LIX to W LXVI)

    NASA Astrophysics Data System (ADS)

    Aggarwal, Kanti M.; Keenan, Francis P.

    2016-09-01

    Calculations of energy levels, radiative rates and lifetimes are reported for eight ions of tungsten, i.e. S-like (W LIX) to F-like (W LXVI). A large number of levels have been considered for each ion and extensive configuration interaction has been included among a range of configurations. For the calculations, the general-purpose relativistic atomic structure package (GRASP) has been adopted, and radiative rates (as well as oscillator strengths and line strengths) are listed for all E1, E2, M1, and M2 transitions of the ions. Comparisons have been made with earlier available experimental and theoretical energies, although these are limited to only a few levels for most ions. Therefore for additional accuracy assessments, particularly for energy levels, analogous calculations have been performed with the flexible atomic code (FAC).

  10. Radiative rates for E1, E2, M1, and M2 transitions in S-like to F-like tungsten ions (W LIX to W LXVI)

    NASA Astrophysics Data System (ADS)

    Aggarwal, Kanti M.; Keenan, Francis P.

    2016-09-01

    Calculations of energy levels, radiative rates and lifetimes are reported for eight ions of tungsten, i.e. S-like (W LIX) to F-like (W LXVI). A large number of levels have been considered for each ion and extensive configuration interaction has been included among a range of configurations. For the calculations, the general-purpose relativistic atomic structure package (GRASP) has been adopted, and radiative rates (as well as oscillator strengths and line strengths) are listed for all E1, E2, M1, and M2 transitions of the ions. Comparisons have been made with earlier available experimental and theoretical energies, although these are limited to only a few levels for most ions. Therefore for additional accuracy assessments, particularly for energy levels, analogous calculations have been performed with the flexible atomic code (FAC).

  11. The role of Domain:Domain Interactions Vs Domain:Water Interactions in the coarse-grained simulations of the E1P to E2P transitions in Ca-ATPase (SERCA)

    PubMed Central

    Andersen, Jens Peter; Woolf, Thomas B.

    2012-01-01

    SERCA is an important model system for understanding the molecular details of conformational change in membrane transport systems. This reflects the large number of solved x-ray structures and the equally large database of mutations that have been assayed. In this computational study we provide a molecular dynamics description of the conformational changes during the E1P -> E2P transitions. This set of states further changes with insertion mutants in the A-M3 linker region. These mutants were experimentally shown to lead to significant shifts in rates between the E1P -> E2P states. Using the population shift framework and dynamic importance sampling method along with coarse-grained representations of the protein, lipid and water, we suggest why these changes are found. The calculations sample on intermediates and suggest that changes in interactions, individual helix interactions and water behavior are key elements in the molecular compositions that underlie shifts in kinetics. In particular, as the insertion length grows, it attracts more water and disrupts domain interactions, creating changes as well at the sites of key helix interactions between the A-Domain and the P-Domain. This provides a conceptual picture that aids understanding of the experimental results. PMID:22422644

  12. 77 FR 77180 - Notice of Transportation Services' OMB Designation, timely return of excess transit benefits to...

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    2012-12-31

    ... Transportation Facilitation, 65 FR 19482 (April 11, 2000). III. Minimum Internal Controls To ensure that funds... return of excess transit benefits to the Treasury, and stakeholder notification of the minimum internal... of the Federal internal controls that now govern the Transit Benefit Program to prevent...

  13. 76 FR 18930 - Medicare Programs: Changes to the End-Stage Renal Disease Prospective Payment System Transition...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ... published a final rule (75 FR 49030 through 49214) in the Federal Register, entitled ``Medicare Program; End... the transition budget-neutrality adjustment. As described in the CY 2011 ESRD PPS final rule (75 FR... FR 49082), we explained that section 1881(b)(14)(E)(iii) of the Act requires that we make...

  14. Radiative rates for E1, E2, M1, and M2 transitions in the Br-like ions Sr IV, Y V, Zr VI, Nb VII, and Mo VIII

    SciTech Connect

    Aggarwal, Kanti M. Keenan, Francis P.

    2015-09-15

    Energies and lifetimes are reported for the lowest 375 levels of five Br-like ions, namely Sr IV, Y V, Zr VI, Nb VII, and Mo VIII, mostly belonging to the 4s{sup 2}4p{sup 5}, 4s{sup 2}4p{sup 4}4ℓ, 4s4p{sup 6}, 4s{sup 2}4p{sup 4}5ℓ, 4s{sup 2}4p{sup 3}4d{sup 2}, 4s4p{sup 5}4ℓ, and 4s4p{sup 5}5ℓ configurations. Extensive configuration interaction has been included and the general-purpose relativistic atomic structure package (GRASP) has been adopted for the calculations. Additionally, radiative rates are listed among these levels for all E1, E2, M1, and M2 transitions. From a comparison with the measurements, the majority of our energy levels are assessed to be accurate to better than 2%, although discrepancies between theory and experiment for a few are up to 6%. An accuracy assessment of the calculated radiative rates (and lifetimes) is more difficult, because no prior results exist for these ions.

  15. 78 FR 50396 - Common Format for Federal Entity Transition Plans

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ..., 78 FR 5310 (Jan. 25, 2013) (to be codified at 47 CFR Part 301), available at http://go.usa.gov/jRHB... whether the revised plan is sufficient. See Technical Panel Rules at Sec. 301.120, 78 FR 5316-17. \\17\\ See... Technical Panel Rules at Sec. 301.10, 78 FR 5315 (cross-referencing Annex O). NTIA will provide the...

  16. Oscillator Strengths and Predissociation Rates for Rydberg Transitions in 12C16O, 13C16O, and 13C18O Involving the E 1Π, B 1Σ+, and W 1Π States

    NASA Astrophysics Data System (ADS)

    Eidelsberg, M.; Sheffer, Y.; Federman, S. R.; Lemaire, J. L.; Fillion, J. H.; Rostas, F.; Ruiz, J.

    2006-08-01

    One of the processes controlling the interstellar CO abundance and the ratio of its isotopologues is photodissociation. Accurate oscillator strengths and predissociation rates for Rydberg transitions are needed for modeling this process. We present results on absorption from the E 1Π-X 1Σ+ (1-0) and B 1Σ+-X 1Σ+ (6-0) bands at 1051 and 1002 Å, respectively, and the vibrational progression W 1Π-X 1Σ+ (v'-0) bands with v'=0-3 at 972, 956, 941, and 925 Å, respectively. The corresponding spectra were acquired at the high resolution (R~30,000) SU5 beam line at the Super ACO Synchrotron in Orsay, France. Spectra were obtained for the 12C16O, 13C 16O, and 13C18O isotopologues. These represent the most complete set of measurements available. Comparison is made with earlier results, both empirical and theoretical. While earlier determinations of oscillator strengths based on absorption from synchrotron radiation tend to be somewhat smaller than ours, the suite of measurements from a variety of techniques agree for the most part, considering the mutual uncertainties. For the bands studied here, their relative weakness, or their significant line widths arising from predissociation, minimizes potential problems from large optical depths at line center in absorption measurements. Predissociating line widths could generally be extracted from the spectra thanks to the profile simulations used in the analysis. In many cases, these simulations allowed us to consider e and f parity levels separately and to determine the dependence of the width on rotational quantum number, J. Our results are consistent with earlier determinations, especially the widths inferred from laser experiments.

  17. 77 FR 32174 - Innovative Transit Workforce Development Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... Federal Transit Administration Innovative Transit Workforce Development Program AGENCY: Federal Transit Administration (FTA), DOT. ACTION: Notice of funding availability (NOFA) for innovative workforce development... (NOFA) for the Innovative Workforce Development Program. This NOFA seeks proposals that promote...

  18. 76 FR 22833 - Swap Data Recordkeeping and Reporting Requirements: Pre-Enactment and Transition Swaps

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    ... Final Rule for Reporting Pre-Enactment Swap Transactions (``Pre-Enactment Swaps IFR''), 75 FR 63080 (Oct... COMMISSION 17 CFR Part 46 Swap Data Recordkeeping and Reporting Requirements: Pre-Enactment and Transition... rules adopted by the Commission shall provide for the reporting of data relating to swaps entered...

  19. 77 FR 43903 - Intent To Prepare an Environmental Impact Statement for Proposed Transit Improvements to the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-26

    ... at http://www.gpo.gov/fdsys/pkg/FR-2011-01-03/pdf/2010-33065.pdf . FOR FURTHER INFORMATION CONTACT... Improvements to the North Red and Purple Lines, Cook County, IL AGENCY: Federal Transit Administration, U.S... Impact Statement (EIS) for the North Red and Purple Line Modernization (RPM) Project in Cook...

  20. 78 FR 50313 - Physical Protection of Irradiated Reactor Fuel in Transit

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... safety. On May 20, 2013 (78 FR 29520), the NRC published the final rule for 10 CFR 73.37, ``Physical... 3150-AI64 Physical Protection of Irradiated Reactor Fuel in Transit AGENCY: Nuclear Regulatory... Transportation of Spent Nuclear Fuel Greater than 100 Grams,'' dated October 10, 2002, and subsequent...

  1. 76 FR 71934 - Tobacco Transition Payment Program; Availability of Current Assessment Methods Determination...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-21

    ... addresses the rulemaking matter covered in a Federal Register document published March 22, 2011 (76 FR 15859... Farm Service Agency Tobacco Transition Payment Program; Availability of Current Assessment Methods... regarding two consolidated determinations with respect to the current methods used to calculate...

  2. 77 FR 28765 - Homeless Emergency Assistance and Rapid Transition to Housing: Emergency Solutions Grants Program...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... (this is a toll-free number). SUPPLEMENTARY INFORMATION: On December 5, 2011, at 76 FR 75954, HUD... URBAN DEVELOPMENT 24 CFR Parts 91 and 576 RIN 2506-AC31 Homeless Emergency Assistance and Rapid Transition to Housing: Emergency Solutions Grants Program and Consolidated Plan Conforming...

  3. 76 FR 55501 - Commonwealth of the Northern Mariana Islands Transitional Worker Classification

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... labor force. Such workers outnumber U.S. citizens and other local residents in most industries central... classification (CW classification) intended to be effective for the duration of the transition period. See 74 FR... Federal Register reopening and extending the public comment period for an additional 30 days. See 74...

  4. 77 FR 38042 - TRICARE; Implementation of TRICARE Transitional Outpatient Payments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-26

    ... Federal Register (73 FR 74945) on December 10, 2008, states Agency will adopt the hold harmless TOPs for... Management Activity (TMA), Medical Benefits and Reimbursement Branch, 16401 East Centretech Parkway,...

  5. 78 FR 1306 - Transition Period Under Section 716 of the Dodd-Frank Wall Street Reform and Consumer Protection Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-08

    ... dealers as swap dealers. \\4\\ Guidance on the Effective Date of Section 716, 77 FR 27465 (May 10, 2012... deems necessary and appropriate.\\12\\ \\11\\ See Further Definition of Swap Dealer, 77 FR 30595 (May 23... Office of the Comptroller of the Currency Transition Period Under Section 716 of the Dodd-Frank...

  6. sup 219 Fr, a transitional reflection asymmetric nucleus

    SciTech Connect

    Liang, C.F.; Paris, P. ); Kvasil, J.; Sheline, R.K. )

    1991-08-01

    Mass-separated sources of {sup 223}Ac (separated as AcF{sub 2}{sup +}) were used to study the level structure of {sup 219}Fr following alpha decay. The levels in {sup 219}Fr are interpreted in terms of {ital K}=1/2{sup {plus minus}}, 3/2{sup {plus minus}}, and 5/2{sup {plus minus}} parity doublet bands which have a natural theoretical explanation in terms of reflection asymmetric models. The 9/2{sup {minus}} ground-state member of the {ital K}=1/2{sup {minus}} band in {sup 219}Fr can be understood in terms of both reflection asymmetry and the collapse of the quadrupole-octupole Nilsson orbitals towards the {ital h}{sub 9/2} orbitals of spherical symmetry. Comparison of the {ital K}=1/2{sup {minus}} ground-state bands in {sup 219}Fr and {sup 221}Fr reveals the details of this transformation. Theoretical analysis of the microscopic structure of several of the positive-parity bands indicates the presence of important Nilsson configurations arising from the shell below.

  7. Influence of shear flow on the Fréedericksz transition in nematic liquid crystals.

    PubMed

    Makarov, D V; Zakhlevnykh, A N

    2006-10-01

    Within the framework of Ericksen-Leslie continuum theory we analyze the influence of shear flow on the magnetic-field-induced Fréedericksz transition in nematic liquid crystal with rodlike molecules. We consider three basic orientational configurations of a nematic planar layer in the uniform magnetic field. Conditions of rigid director coupling on the boundaries of the layer and constant shear flow gradient inside the layer are used. We exhibit some flow aligning effects for nematic liquid crystals with various ratio of rotary viscosities and investigate how unequal elastic constants (elastic anisotropy) alter the magnetic Fréedericksz transition in sheared nematics. Our calculations predict that surface boundary effects in nematic films and magnetic field action lead to existence of stationary flow regimes in the so-called nonflow aligning nematics, otherwise, surface and magnetic forces extend the range of viscous coefficient values corresponding to the flow aligning regimes. We show that imposing of shear flow on the Fréedericksz transition leads to a threshold behavior or to a "smoothing" of the transition. It depends on the orientation of the nematic layer in magnetic field and magnitudes of rotary viscous coefficients. PMID:17155081

  8. Jet-gas interactions and hotspots in FR I/II transition sources

    NASA Astrophysics Data System (ADS)

    Worrall, Diana; Birkinshaw, Mark

    2016-07-01

    Sources with intermediate FR I/II morphologies, and with powers in the decade straddling the FR I/II boundary, provide an opportunity to understand triggers responsible for the different workings of the two classes. Illustrated by deep Chandra observations of several sources, this presentation will show evidence that the physics changes within the transition range, and the work done in driving shocks can exceed that in evacuating the cavities common in FR I sources. Hotspots can be absent, seen only on one side (jet-side or counter-jet-side), or both, in which case X-ray/radio correspondence can be very different on the two sides. Evidence will be shown for radio-emitting plasma running along boundaries between gas of different temperature, apparently lubricating the gas flows and inhibiting heat transfer.

  9. 75 FR 45677 - Draft Regulatory Guide, DG-1216,”Plant-Specific Applicability of Transition Break Size Specified...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    .... SUPPLEMENTARY INFORMATION: On June 28, 2010 (75 FR 36700), the NRC published a notice of issuance and... COMMISSION Draft Regulatory Guide, DG-1216,''Plant-Specific Applicability of Transition Break Size Specified... . The Draft Regulatory Guide, DG-1216, ``Plant- Specific Applicability of Transition Break...

  10. 76 FR 27168 - Airmen Transition to Experimental or Unfamiliar Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Airmen Transition to Experimental or Unfamiliar Airplanes AGENCY: Federal... accomplish the test program using AC 90-89. This AC may also be useful in planning the transition to...

  11. 77 FR 37346 - Export Control Reform Transition Plan

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-21

    ... will transition from the jurisdiction of the Department of State to the Department of Commerce. The... transition of items to the jurisdiction of the Department of Commerce. The revisions ] to this rule are part of the Department of State's retrospective plan under E.O. 13563 completed on August 17, 2011....

  12. 75 FR 27165 - Conservation Reserve Program; Transition Incentives Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-14

    ...The Commodity Credit Corporation (CCC) is amending the Conservation Reserve Program (CRP) regulations to add provisions for incentives to retired or retiring owners or operators to transition land enrolled in CRP to a beginning or socially disadvantaged farmer or rancher for production. The Transition Incentives Program involves new and mandatory provisions for CRP authorized by the Food,......

  13. 78 FR 46905 - Tobacco Transition Program; Final Assessment Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-02

    ... information about the final quarterly assessments for the Tobacco Transition Program (TTP). Through the Tobacco Transition Payment Program (TTPP), which is part of the TTP, eligible former tobacco quota holders..., 2014. Also, as specified in the TTP regulations, the final two calendar quarterly payments are both...

  14. 78 FR 16675 - First Technology Transitions; Policy Task Force Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-18

    ... COMMISSION First Technology Transitions; Policy Task Force Workshop AGENCY: Federal Communications Commission... Policy Task Force, at (202) 418-1438 or rebekah.goodheart@fcc.gov . SUPPLEMENTARY INFORMATION: The FCC... Policy Task Force, see Ex Parte Meetings with the Technology Transitions Policy Task Force, Public...

  15. A Two-Photon E1-M1 Optical Clock

    NASA Astrophysics Data System (ADS)

    Alden, Emily A.

    Innovations in precision frequency measurement advance popular technologies such as global positioning systems (GPS), permit the testing of fundamental physics constants, and have the potential to measure local variations in gravity. Driving optical transitions for frequency measurement using an E1-M1 excitation scheme in a hot mercury (Hg) vapor cell is viable and could be the basis of a portable optical frequency standard with comparable accuracy to the most precise atomic clocks in the world. This dissertation explores the fundamental physics of the new E1-M1 method of high-precision frequency measurement in an optical, atomic clock and describes the construction of a high-power E1-M1 clock laser. The value of this new scheme compared to existing optical frequency standards is the simplicity and portability of the experimental setup. Such an optical frequency standard would permit frequency measurement in far-flung locations on earth and in space. Analysis of both the E1-M1 optical transition and thermal properties of the candidate clock atoms are presented. These models allow a stability estimate of an E1-M1 optical clock and recommend experimental settings to optimize the standard. The experimental work that has been performed in pursuit of observing the E1-M1 clock transition in Hg is also discussed. An optical clock operates by making a precision frequency measurement of a laser that has been brought into resonance with a clock atom's oscillator: a high quality atomic level transition. Group II type atoms, such as Hg, have the 1S0-3P0 transition that is an ideal basis for a clock. The E1-M1 excitation is performed by driving the two-photon allowed transition 1S0-3P1-3P0. This is in contrast to the single-photon E1 transition used in other systems. Single-photon schemes must use ultracold atoms to reduce atomic motion to attain high levels of accuracy. Driving the clock transition with a pair of degenerate counter-propagating photons in an E1-M1 scheme

  16. Energy surface and minimum energy paths for Fréedericksz transitions in bistable cholesteric liquid crystals

    NASA Astrophysics Data System (ADS)

    Ivanov, A. V.; Bessarab, P. F.; Aksenova, E. V.; Romanov, V. P.; Uzdin, V. M.

    2016-04-01

    The multidimensional energy surface of a cholesteric liquid crystal in a planar cell is investigated as a function of spherical coordinates determining the director orientation. Minima on the energy surface correspond to the stable states with particular director distribution. External electric and magnetic fields deform the energy surface and positions of minima. It can lead to the transitions between states, known as the Fréedericksz effect. Transitions can be continuous or discontinuous depending on parameters of the liquid crystal which determine an energy surface. In a case of discontinuous transition when a barrier between stable states is comparable with the thermal energy, the activation transitions may occur, and it leads to the modification of characteristics of the Fréedericksz effect with temperature without explicit temperature dependencies of liquid crystal parameters. A minimum energy path between stable states on the energy surface for the Fréedericksz transition is found using the geodesic nudged elastic band method. Knowledge of this path, which has maximal statistical weight among all other paths, gives the information about a barrier between stable states and configuration of director orientation during the transition. It also allows one to estimate the stability of states with respect to the thermal fluctuations and their lifetime when the system is close to the Fréedericksz transition.

  17. Transitions.

    ERIC Educational Resources Information Center

    Nathanson, Jeanne H., Ed.

    1993-01-01

    This theme issue on transitions for individuals with disabilities contains nine papers discussing transition programs and issues. "Transition Issues for the 1990s," by Michael J. Ward and William D. Halloran, discusses self-determination, school responsibility for transition, continued educational engagement of at-risk students, and service…

  18. 75 FR 70966 - Transit Asset Management (TAM) Pilot Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-19

    ... Federal Transit Administration (FTA) is soliciting proposals from public transportation providers, state... be replicated to improve transportation asset management at the nation's rail and bus public transportation agencies. Public sector applicants may partner with asset management system suppliers; however...

  19. Transitions.

    ERIC Educational Resources Information Center

    Field, David; And Others

    1992-01-01

    Includes four articles: "Career Aspirations" (Field); "Making the Transition to a New Curriculum" (Baker, Householder); "How about a 'Work to School' Transition?" (Glasberg); and "Technological Improvisation: Bringing CNC to Woodworking" (Charles, McDuffie). (SK)

  20. 77 FR 38556 - Export Control Reform Transition Plan

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE 22 CFR Parts 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, and 130 ] Export Control Reform Transition Plan Correction In proposed rule document 2012-15070 appearing on pages 37346-37349 in the issue of Thursday,...

  1. Transition.

    ERIC Educational Resources Information Center

    Thompson, Sandy, Ed.; And Others

    1990-01-01

    This "feature issue" focuses on transition from school to adult life for persons with disabilities. Included are "success stories," brief program descriptions, and a list of resources. Individual articles include the following titles and authors: "Transition: An Energizing Concept" (Paul Bates); "Transition Issues for the 1990s" (William Halloran…

  2. Energies and E1, M1, E2, M2 transition rates for states of the 2s{sup 2}2p, 2s2p{sup 2}, and 2p{sup 3} configurations in boron-like ions between N III and Zn XXVI

    SciTech Connect

    Rynkun, P.; Joensson, P.; Gaigalas, G.; Froese Fischer, C.

    2012-07-15

    Energies, E1, M1, E2, M2 transition rates, line strengths, oscillator strengths, and lifetimes from relativistic configuration interaction calculations are reported for the states of the (1s{sup 2})2s{sup 2}2p, 2s2p{sup 2}, and 2p{sup 3} configurations in all boron-like ions between N III and Zn XXVI. Valence, core-valence, and core-core correlation effects were accounted for through single-double multireference (SD-MR) expansions to increasing sets of active orbitals.

  3. Energies and E1, M1, E2, and M2 transition rates for states of the 2s22p3, 2s2p4, and 2p5 configurations in nitrogen-like ions between F III and Kr XXX

    NASA Astrophysics Data System (ADS)

    Rynkun, P.; Jönsson, P.; Gaigalas, G.; Froese Fischer, C.

    2014-03-01

    Based on relativistic wavefunctions from multiconfiguration Dirac-Hartree-Fock and configuration interaction calculations, E1, M1, E2, and M2 transition rates, weighted oscillator strengths, and lifetimes are evaluated for the states of the (1s2)2s22p3,2s2p4, and 2p5 configurations in all nitrogen-like ions between F III and Kr XXX. The wavefunction expansions include valence, core-valence, and core-core correlation effects through single-double multireference expansions to increasing sets of active orbitals. The computed energies agree very well with experimental values, with differences of only 300-600 cm-1 for the majority of the levels and ions in the sequence. Computed transitions rates are in close agreement with available data from MCHF-BP calculations by Tachiev and Froese Fischer [G.I. Tachiev, C. Froese Fischer, A&A 385 (2002) 716].

  4. 78 FR 31542 - FCC Technology Transitions Policy Task Force Seeks Comment on Potential Trials

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ...) or (2) by filing paper copies. See Electronic Filing of Documents in Rulemaking Proceedings, 63 FR... FR 78384, 76 FR 76623, 77 FR 26987, December 8, 2011, December 16, 2011, May 8, 2012. The... filing paper copies. See Electronic Filing of Documents in Rulemaking Proceedings, 63 FR 24121...

  5. 26 CFR 1.367(e)-1 - Distributions described in section 367(e)(1).

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 4 2013-04-01 2013-04-01 false Distributions described in section 367(e)(1). 1.367(e)-1 Section 1.367(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Effects on Corporation § 1.367(e)-1 Distributions described in section 367(e)(1)....

  6. Gene coding for the E1 endoglucanase

    DOEpatents

    Thomas, Steven R.; Laymon, Robert A.; Himmel, Michael E.

    1996-01-01

    The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol.

  7. Gene coding for the E1 endoglucanase

    DOEpatents

    Thomas, S.R.; Laymon, R.A.; Himmel, M.E.

    1996-07-16

    The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol. 6 figs.

  8. A Conserved Regulatory Module at the C Terminus of the Papillomavirus E1 Helicase Domain Controls E1 Helicase Assembly

    PubMed Central

    Schuck, Stephen

    2014-01-01

    ABSTRACT Viruses frequently combine multiple activities into one polypeptide to conserve coding capacity. This strategy creates regulatory challenges to ascertain that the combined activities are compatible and do not interfere with each other. The papillomavirus E1 protein, as many other helicases, has the intrinsic ability to form hexamers and double hexamers (DH) that serve as the replicative DNA helicase. However, E1 also has the more unusual ability to generate local melting by forming a double trimer (DT) complex that can untwist the double-stranded origin of DNA replication (ori) DNA in preparation for DH formation. Here we describe a switching mechanism that allows the papillomavirus E1 protein to form these two different kinds of oligomers and to transition between them. We show that a conserved regulatory module attached to the E1 helicase domain blocks hexamer and DH formation and promotes DT formation. In the presence of the appropriate trigger, the inhibitory effect of the regulatory module is relieved and the transition to DH formation can occur. IMPORTANCE This study provides a mechanistic understanding into how a multifunctional viral polypeptide can provide different, seemingly incompatible activities. A conserved regulatory sequence module attached to the AAA+ helicase domain in the papillomavirus E1 protein allows the formation of different oligomers with different biochemical activities. PMID:25378487

  9. Energies and E1, M1, E2, and M2 transition rates for states of the 2s{sup 2}2p{sup 3}, 2s2p{sup 4}, and 2p{sup 5} configurations in nitrogen-like ions between F III and Kr XXX

    SciTech Connect

    Rynkun, P.; Jönsson, P.; Gaigalas, G.; Froese Fischer, C.

    2014-03-15

    Based on relativistic wavefunctions from multiconfiguration Dirac–Hartree–Fock and configuration interaction calculations, E1, M1, E2, and M2 transition rates, weighted oscillator strengths, and lifetimes are evaluated for the states of the (1s{sup 2})2s{sup 2}2p{sup 3},2s2p{sup 4}, and 2p{sup 5} configurations in all nitrogen-like ions between F III and Kr XXX. The wavefunction expansions include valence, core–valence, and core–core correlation effects through single–double multireference expansions to increasing sets of active orbitals. The computed energies agree very well with experimental values, with differences of only 300–600 cm{sup −1} for the majority of the levels and ions in the sequence. Computed transitions rates are in close agreement with available data from MCHF-BP calculations by Tachiev and Froese Fischer [G.I. Tachiev, C. Froese Fischer, A and A 385 (2002) 716].

  10. 26 CFR 1.367(e)-1 - Distributions described in section 367(e)(1).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 4 2010-04-01 2010-04-01 false Distributions described in section 367(e)(1). 1.367(e)-1 Section 1.367(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Effects on Corporation § 1.367(e)-1 Distributions described in section 367(e)(1). (a) Purpose and...

  11. 75 FR 12962 - Transitional Safe Harbor Protection for Treatment by the Federal Deposit Insurance Corporation as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ..., 2010 (74 FR 59066) . FOR FURTHER INFORMATION CONTACT: Michael Krimminger, Office of the Chairman, 202... concerns, the FDIC published an Interim Final Rule on November 17, 2010 (74 FR 59066) that addressed... Connection With a Securitization or Participation After March 31, 2010, 75 FR 934, January 7, 2010)....

  12. 75 FR 14330 - Transitional Safe Harbor Protection for Treatment by the Federal Deposit Insurance Corporation as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-25

    ...) is correcting a final rule that appeared in the Federal Register of March 18, 2010 (75 FR 12962). The... the final rule published in the Federal Register of March 18, 2010 (75 FR 12962), an incorrect date... changes, the interim rule published on November 17, 2009 (74 FR 59066) . 2. On page 12963, the...

  13. 75 FR 53521 - Procedures for the Handling of Retaliation Complaints Under the National Transit Systems Security...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... (Secretary's Order 5-2007, 72 FR 31160 (June 5, 2007)). Hearings on determinations by the Assistant Secretary... decided by the ARB (Secretary's Order 1-2010 (Jan. 15, 2010), 75 FR 3924-01 (Jan. 25, 2010)). Subpart A.... 1142 and 49 U.S.C. 20109; Secretary of Labor's Order No. 5-2007, 72 FR 31160 (June 5, 2007);...

  14. 75 FR 1632 - Public Housing Assessment System (PHAS): Asset Management Transition Year 2 Information

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-12

    ..., 1998 (63 FR 46596). Prior to 1998, PHAs were evaluated by HUD under the Public Housing Management... remedial action. By final rule published on January 11, 2000 (65 FR 1712), HUD amended the PHAS regulations... Operating Fund Program are found at 24 CFR part 990, were published on September 19, 2005 (70 FR...

  15. 77 FR 71287 - CNMI-Only Transitional Worker Numerical Limitation for Fiscal Year 2013

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-30

    ... includes CW-1 for principal workers and CW-2 for spouses and minor children). See 76 FR 55502 (Sept. 7... economic conditions. See 76 FR at 55510. As such, DHS opted to publish any future annual numerical... phase-in of Federal immigration law. See 76 FR at 55510. The numerical limitations set forth in...

  16. 76 FR 15859 - Tobacco Transition Payment Program; Cigar and Cigarette Per Unit Assessments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... published December 10, 2010 (75 FR 76921-76923), explaining this policy regarding Step A and clarifying the.... The final rule implementing TTPP was published on February 10, 2005 (70 FR ] 7007-7014). There is a... was amended by a final rule published in the Federal Register on April 4, 2005 (70 FR...

  17. 78 FR 18725 - Homeless Emergency Assistance and Rapid Transition to Housing: Rural Housing Stability Assistance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... with a proposed rule, which published on April 20, 2010 (75 FR 20541) and titled ``Defining Homeless...,'' was published on December 5, 2011 (76 FR 75994). On December 5, 2011, HUD also published an interim rule, titled the ``Emergency Solutions Grants Rule.'' (See 76 FR 75954.) This interim rule...

  18. 77 FR 45421 - Homeless Emergency Assistance and Rapid Transition to Housing: Continuum of Care Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-31

    ... proposed rule, entitled ``Defining Homeless'' on April 20, 2010 (75 FR 20541), which was followed by a final rule that was published on December 5, 2011 (76 FR 75994). The Defining Homeless rule clarified... Emergency Solutions Grants program (76 FR 75954). This interim rule established the program requirements...

  19. 75 FR 62695 - Physical Protection of Irradiated Reactor Fuel in Transit

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-13

    ..., 1979 (44 FR 34466), NRC published an interim final rule in the Federal Register that established its... 3, 1980 (45 FR 37399). The current Sec. 73.37 has changed little since its promulgation in 1980... petition and a request for public comment on September 13, 1999 (64 FR 49410). The Commission review...

  20. 78 FR 29519 - Physical Protection of Irradiated Reactor Fuel in Transit

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... Register (44 FR 34466) an interim final rule that established requirements for the physical protection of... June 3, 1980 (45 FR 37399). Section 73.37 has changed little since its promulgation in 1980. The... for public comment a notice of receipt of PRM-73-10 on September 13, 1999 (64 FR 49410). The...

  1. Cosmographic bounds on the cosmological deceleration-acceleration transition redshift in f(R) gravity

    NASA Astrophysics Data System (ADS)

    Capozziello, Salvatore; Farooq, Omer; Luongo, Orlando; Ratra, Bharat

    2014-08-01

    We examine the observational viability of a class of f(R) gravity cosmological models. Particular attention is devoted to constraints from the recent observational determination of the redshift of the cosmological deceleration-acceleration transition. Making use of the fact that the Ricci scalar is a function of redshift z in these models, R =R(z), and so is f(z), we use cosmography to relate a f(z) test function evaluated at higher z to late-time cosmographic bounds. First, we consider a model-independent procedure to build up a numerical f(z) by requiring that at z=0 the corresponding cosmological model reduces to standard ΛCDM. We then infer late-time observational constraints on f(z) in terms of bounds on the Taylor expansion cosmographic coefficients. In doing so we parametrize possible departures from the standard ΛCDM model in terms of a two-parameter logarithmic correction. The physical meaning of the two parameters is also discussed in terms of the post-Newtonian approximation. Second, we provide numerical estimates of the cosmographic series terms by using type Ia supernova apparent magnitude data and Hubble parameter measurements. Finally, we use these estimates to bound the two parameters of the logarithmic correction. We find that the deceleration parameter in our model changes sign at a redshift consistent with what is observed.

  2. Adenovirus E1B 19-kilodalton protein overcomes the cytotoxicity of E1A proteins.

    PubMed Central

    White, E; Cipriani, R; Sabbatini, P; Denton, A

    1991-01-01

    Infection with adenovirus mutants carrying either point mutations or deletions in the coding region for the 19-kDa E1B gene product (19K protein) causes degradation of host cell and viral DNAs (deg phenotype) and enhanced cytopathic effect (cyt phenotype). Therefore, one function of the E1B 19K protein is to protect nuclear DNA integrity and preserve cytoplasmic architecture during productive adenovirus infection. When placed in the background of a virus incapable of expressing a functional E1A gene product, however, E1B 19K gene mutations do not result in the appearance of the cyt and deg phenotypes. This demonstrated that expression of the E1A proteins was responsible for inducing the appearance of the cyt and deg phenotypes. By constructing a panel of viruses possessing E1A mutations spanning each of the three E1A conserved regions in conjunction with E1B 19K gene mutations, we mapped the induction of the cyt and deg phenotypes to the amino-terminal region of E1A. Viruses that fail to express conserved region 3 (amino acids 140 to 185) and/or 2, (amino acids 121 to 185) or nonconserved sequences between conserved regions 2 and 1 of E1A (amino acids 86 to 120) were still capable of inducing cyt and deg. This indicated that activities associated with these regions, such as transactivation and binding to the product of the retinoblastoma susceptibility gene, were dispensable for induction of E1A-dependent cytotoxic effects. In contrast, deletion of sequences in the amino terminus of E1A (amino acids 22 to 107) resulted in extragenic suppression of the cyt and deg phenotypes. Therefore, a function affected by deletion of amino acids 22 to 86 of E1A is responsible for exerting cytotoxic effects in virally infected cells. Furthermore, transient high-level expression of the E1A region using a cytomegalovirus promoter plasmid expression vector was sufficient to induce the cyt and deg phenotypes, demonstrating that E1A expression alone is sufficient to exert these

  3. Identification and characterization of an hnRNP E1 translational silencing motif

    PubMed Central

    Brown, Andrew S.; Mohanty, Bidyut K.; Howe, Philip H.

    2016-01-01

    Non-canonical transforming growth factor β (TGFβ) signaling through protein kinase B (Akt2) induces phosphorylation of heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) at serine-43 (p-hnRNP E1). This post-translational modification (PTM) of hnRNP E1 promotes its dissociation from a 3′ untranslated region (UTR) nucleic acid regulatory motif, driving epithelial to mesenchymal transition (EMT) and metastasis. We have identified an hnRNP E1 consensus-binding motif and genomically resolved a subset of genes in which it is contained. This study characterizes the binding kinetics of the consensus-binding motif and hnRNP E1, its various K-homology (KH) domains and p-hnRNP E1. Levels of p-hnRNP E1 are highly upregulated in metastatic cancer cells and low in normal epithelial tissue. We show a correlation between this PTM and levels of Akt2 and its activated form, phosphorylated serine-474 (p-Akt2). Using cellular progression models of metastasis, we observed a signature high level of Akt2, p-Akt2 and p-hnRNP E1 protein expression, coupled to a significantly reduced level of total hnRNP E1 in metastatic cells. Genes that are translationally silenced by hnRNP E1 and expressed by its dissociation are highly implicated in the progression of EMT and metastasis. This study provides insight into a non-canonical TGFβ signaling cascade that is responsible for inducing EMT by aberrant expression of hnRNP E1 silenced targets. The relevance of this system in metastatic progression is clearly shown in cellular models by the high abundance of p-hnRNP E1 and low levels of hnRNP E1. New insights provided by the resolution of this molecular mechanism provide targets for therapeutic intervention and give further insight into the role of the TGFβ microenvironment. PMID:27067543

  4. 76 FR 69119 - Commonwealth of the Northern Mariana Islands Transitional Worker Classification: Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ..., published in the Federal Register on September 7, 2011 at 76 FR 55502, DHS intended to revise only the.... 107-296, 116 Stat. 2135 (6 U.S.C. 1 et seq.), E.O. 12356, 47 FR 14874, 15557, 3 CFR, 1982 Comp., p... Classification: Correction AGENCY: U.S. Citizenship and Immigration Services, DHS. ACTION: Final rule;...

  5. 77 FR 7080 - Changes To Implement Transitional Program for Covered Business Method Patents

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-10

    ... Flexibility Analysis for Patent-Related Regulations, 71 FR 67109 (Nov. 20, 2006), 1313 Off. Gaz. Pat. Office... Regulations, 71 FR at 67112 (Nov 20, 2006), 1313 Off. Gaz. Pat. Office at 63 (Dec. 12, 2006). As discussed... Covered Business Method Patents AGENCY: United States Patent and Trademark Office, Commerce....

  6. 76 FR 19710 - Tobacco Transition Payment Program; Cigar and Cigarette Per Unit Assessments; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-08

    ... 0560-AI12 and include the volume, date, and page number (March 22, 2011, 76 FR 15859-15864) of the..., 2011, CCC published a Request for Comments (76 FR 15859-15864) requesting comments about the... Payment Program; Cigar and Cigarette Per Unit Assessments; Correction AGENCY: Commodity Credit...

  7. 76 FR 1376 - Physical Protection of Irradiated Reactor Fuel in Transit Extension of Comment Period

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ...: Extension of comment period. SUMMARY: On October 13, 2010 (75 FR 62695), the U.S. Nuclear Regulatory... was published on October 13, 2010 (75 FR 62695) and the public comment period was scheduled to expire... nuclear fuel'' (SNF) are used interchangeably). This proposed rule would establish generically...

  8. 76 FR 31639 - Comment Request for Information Collection for Enhanced Transitional Jobs Demonstration, New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ... Request for Information Collection for Enhanced Transitional Jobs Demonstration, New Collection AGENCY... the Enhanced Transitional Jobs Demonstration project (ETJD). A copy of the proposed information...@dol.gov . SUPPLEMENTARY INFORMATION: I. Background In applying for the Enhanced Transitional...

  9. 76 FR 44394 - Public Transportation on Indian Reservations Program; Tribal Transit Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... Federal Transit Administration Public Transportation on Indian Reservations Program; Tribal Transit... availability of $15,075,000 in funding provided by the Public Transportation on Indian Reservations Program... establishing the Public Transportation on Indian Reservations Program (Tribal Transit Program) (TTP)....

  10. 77 FR 63920 - New Jersey Transit Corporation-Acquisition Exemption-Norfolk Southern Railway Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... Surface Transportation Board New Jersey Transit Corporation--Acquisition Exemption--Norfolk Southern Railway Company The New Jersey Transit Corporation (NJ Transit), a noncarrier, has filed a verified notice..., N.J., from milepost 8.616 to milepost 9.905 (the Line). NJ Transit states that, under the...

  11. 78 FR 10253 - Federal Fiscal Year 2013 Annual List of Certifications and Assurances for Federal Transit...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-13

    ..., Allocations, Program Information and Interim Guidance,'' 77 FR 63670, October 16, 2012 (FTA FY 2013... marijuana use for medical or recreational purposes, your Applicant must comply with Federal (not...

  12. 76 FR 59495 - Presidential Determination on Major Illicit Drug Transit or Major Illicit Drug Producing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-26

    ... Sig.) THE WHITE HOUSE, Washington, September 15, 2011 [FR Doc. 2011-24887 Filed 9-23-11; 11:15 am... necessarily an adverse reflection of its government's counternarcotics efforts or level of cooperation...

  13. 78 FR 36431 - Safety Zone; Inbound Transit of M/V TEAL, Savannah River; Savannah, GA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-18

    ... DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking STS Ship to... River to the Georgia Ports Authority, Garden City Terminal Container Berth 8 (CB8). This safety...

  14. Prostaglandin E1 in hand angiography

    SciTech Connect

    Levy, J.M.; Joseph, R.B.; Bodell, L.S.; Nykamp, P.W.; Hessel, S.J.

    1983-11-01

    Prostaglandin E1 (PG1) is a rapid, potent vasodilator which, when infused into the arterial system in low doses by bolus injection, has no significant systemic effects and has a relatively long duration of action. Sixty-three hand angiograms were done on 55 patients, comparing PGE1 to tolazoline and to angiograms done with no vasodilation. There was no significant difference between PGE1 and tolazoline in digital artery opacification; however, venous opacification was very significantly better with PGE1. PGE1 should be a drug of choice in hand angiography.

  15. Origin of replication of colicin E1 plasmid DNA.

    PubMed Central

    Tomizawa, J I; Ohmori, H; Bird, R E

    1977-01-01

    Cleavage maps of colicin E1 plasmid DNA and its smaller derivative, pNT1 DNA, were constructed by using restriction endonucleases. The nucleotide sequence of a region that contains the orgin of replication was determined. The site of the nucleotide from which DNA replication is initiated was determined with 6S L-fragments, the DNA fragment first made on colicin E1 plasmid DNA. The fragments were labeled with [gamma-32P]ATP and polynucleotide 5'-hydroxyl-kinase (ATP:5'-dephosphopolynucleotide 5'-phosphotransferase, EC 2.7.1.78) at the 5'-OH groups which were uncovered by alkali treatment. The site is one of three consecutive nucleotides, dA, dA, and dC, located at a unique position. One or a few rA residues were found to be attached to some of the DNA molecules. The transition from the primer RNA to DNA occurs in a region consisting of a segment of five A residues. Both sides of this segment are rich in G and C. Images PMID:325558

  16. 75 FR 38506 - Office of Postsecondary Education: Overview Information; Coordinating Center for Transition and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ... Postsecondary Programs for Students With Intellectual Disabilities; Notice Inviting Applications for New Awards... transition and postsecondary programs for students with intellectual disabilities, including institutions that have grants authorized under the Transition Programs for Students with Intellectual...

  17. 75 FR 14243 - Pipeline Safety: Girth Weld Quality Issues Due to Improper Transitioning, Misalignment, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-24

    ... Improper Transitioning, Misalignment, and Welding Practices of Large Diameter Line Pipe AGENCY: Pipeline... girth weld failures due to welding quality issues. Misalignment during welding of large diameter line... bevel and wall thickness transitions, and other improper welding practices that occurred...

  18. 77 FR 1779 - Meeting and Webinar on Integrated Dynamic Transit Operations; Notice of Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-11

    ... for Mobility program is to improve public transportation by increasing transit productivity... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Meeting and Webinar on Integrated Dynamic Transit Operations; Notice of Public Meeting AGENCY:...

  19. 77 FR 52131 - FY 2012 Discretionary Funding Opportunity: Paul S. Sarbanes Transit in Parks Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-28

    ... agreements, transit-related intelligent transportation systems, relocation assistance, acquiring replacement... qualifying alternative transportation systems, including the development of related...

  20. 77 FR 28421 - Supplemental Draft Environmental Impact Statement for the Central Corridor Light Rail Transit...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-14

    ... Light Rail Transit Project, Minneapolis and Saint Paul, MN AGENCY: Federal Transit Administration (FTA... prepare a Supplemental Draft Environmental Impact Statement (SDEIS) for the Central Corridor Light Rail... miles long and consists of 23 Central Corridor Light Rail Transit (LRT) stations. The SDEIS...

  1. 77 FR 14465 - Public Transportation on Indian Reservations Program; Tribal Transit Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-09

    ... Federal Transit Administration Public Transportation on Indian Reservations Program; Tribal Transit... Surface and Air Transportation Programs Extension Act of 2011, Public Law No.112-30 continues the... establishing the Public Transportation on Indian Reservations Program (Tribal Transit Program or TTP)....

  2. 78 FR 13748 - Environmental Impact Statement for the East San Fernando Valley Transit Corridor Project, Los...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ...The Federal Transit Administration (FTA) and the Los Angeles County Metropolitan Transportation Authority (LACMTA) are issuing this Notice of Intent (NOI) to advise other agencies and the public that they will jointly prepare an Environmental Impact Statement (EIS) for proposed transit improvements in the East San Fernando Valley Transit Project Corridor in Los Angeles County, California. The......

  3. 76 FR 60587 - Environmental Impact Statement; North Corridor Transit Project, Seattle (WA) Metropolitan Area...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-29

    ... County. ] Alternatives Analysis and Results The North Corridor Transit Project Alternatives Analysis (AA... for transit projects seeking New Starts funding (Title 49 United States Code 5309.) The AA report also... Supplemental EIS on the Regional Transit Long-Range Plan (June 2005). The North Corridor AA considered...

  4. 77 FR 64120 - Agency Information Collection Activities: Petition for CNMI-Only Nonimmigrant Transition Worker...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-18

    ... Register on August 3, 2012, at 77 FR 46446, allowing for a 60-day public comment period. USCIS did receive... via email at oira_submission@omb.eop.gov and via the Federal eRulemaking Portal Web site at http://www... submissions will be posted, without change, to the Federal eRulemaking Portal at...

  5. 75 FR 60494 - Notice of Proposed Guidance and Request for Comment on the Federal Transit Administration's...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-30

    ... Privacy Act Statement in the Federal Register published April 11, 2000, (65 FR 19477), or you may visit... (TEAM system), the development of pre-applications or white papers, the development of formal... (MPR) in TEAM and clarifies ] the development of Quarterly Narrative Reports. Section 4 also...

  6. 75 FR 20541 - Homeless Emergency Assistance and Rapid Transition to Housing: Defining “Homeless”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-20

    ... the Emergency Solutions Grant Program and the Rural Housing Stability Program. The HEARTH Act also... Rural Housing Stability program replaces the Rural Homelessness Grant program. The HEARTH Act also... the proposal by name and docket number (FR-5333-P-01) and must be sent to: HUD Desk Officer, Office...

  7. 76 FR 75994 - Homeless Emergency Assistance and Rapid Transition to Housing: Defining “Homeless”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    .... The April 2010 Proposed Rule On April 20, 2010, HUD published a proposed rule (75 FR 20541) to... statutory language of the definition in section 103 of the McKinney- Vento Act, as amended by the HEARTH Act... consistent with HUD's longstanding interpretation of the statutory language ``lacks a fixed, regular...

  8. 77 FR 58915 - Presidential Determination on Major Illicit Drug Transit or Major Illicit Drug Producing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... Register. (Presidential Sig.) THE WHITE HOUSE, Washington, September 14, 2012. [FR Doc. 2012-23640 Filed 9..., Dominican Republic, Ecuador, El Salvador, Guatemala, Haiti, Honduras, India, Jamaica, Laos, Mexico... Nations Office on Drugs and Crime (UNODC) estimate that cultivation in Helmand decreased between 35 and...

  9. 78 FR 58855 - Presidential Determination on Major Drug Transit or Major Illicit Drug Producing Countries for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... WHITE HOUSE, Washington, September 13, 2013. [FR Doc. 2013-23433 Filed 9-24-13; 8:45 am] Billing code..., Honduras, India, Jamaica, Laos, Mexico, Nicaragua, Pakistan, Panama, Peru, and Venezuela. A country's... citizen safety by fostering a wide range of crime prevention programs. Although the problems are...

  10. 76 FR 17629 - Applications for New Awards; Transition to Teaching Grant Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-30

    ... December 15, 2010 (75 FR 78486). For FY 2011 and any subsequent year in which we make awards from the list... Science, Technology, Engineering, and Mathematics (STEM) Education. Projects that are designed to address... workforce. According to the U.S. Department of Education, Institute of Education Sciences, nearly...

  11. Adenovirus E1A/E1B Transformed Amniotic Fluid Cells Support Human Cytomegalovirus Replication

    PubMed Central

    Krömmelbein, Natascha; Wiebusch, Lüder; Schiedner, Gudrun; Büscher, Nicole; Sauer, Caroline; Florin, Luise; Sehn, Elisabeth; Wolfrum, Uwe; Plachter, Bodo

    2016-01-01

    The human cytomegalovirus (HCMV) replicates to high titers in primary human fibroblast cell cultures. A variety of primary human cells and some tumor-derived cell lines do also support permissive HCMV replication, yet at low levels. Cell lines established by transfection of the transforming functions of adenoviruses have been notoriously resistant to HCMV replication and progeny production. Here, we provide first-time evidence that a permanent cell line immortalized by adenovirus type 5 E1A and E1B (CAP) is supporting the full HCMV replication cycle and is releasing infectious progeny. The CAP cell line had previously been established from amniotic fluid cells which were likely derived from membranes of the developing fetus. These cells can be grown under serum-free conditions. HCMV efficiently penetrated CAP cells, expressed its immediate-early proteins and dispersed restrictive PML-bodies. Viral DNA replication was initiated and viral progeny became detectable by electron microscopy in CAP cells. Furthermore, infectious virus was released from CAP cells, yet to lower levels compared to fibroblasts. Subviral dense bodies were also secreted from CAP cells. The results show that E1A/E1B expression in transformed cells is not generally repressive to HCMV replication and that CAP cells may be a good substrate for dense body based vaccine production. PMID:26848680

  12. 75 FR 5172 - Solicitation of Nominations for Members of the Transit Rail Advisory Committee for Safety

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ..., transit agencies, rider advocacy groups, State safety oversight agencies, and industry associations... interested in keeping TRACS free of individuals who currently are registered Federal lobbyists. The...

  13. 75 FR 53639 - Best Practices for Transit, Transshipment, and Reexport of Items Subject to the Export...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-01

    ... Bureau of Industry and Security Best Practices for Transit, Transshipment, and Reexport of Items Subject... public comments on a set of proposed ``Best Practices for Transit, Transshipment, and Reexport of Items... dialogue with industry regarding new transshipment principles and best practices that complement...

  14. 75 FR 27114 - Public Transportation on Indian Reservations Program; Tribal Transit Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... Federal Transit Administration Public Transportation on Indian Reservations Program; Tribal Transit... availability of $15,074,963 million in funding provided by the Public Transportation on Indian Reservations.... Overview Section 3013 of SAFETEA-LU, amended 49 U.S.C. 5311(c) by establishing the Public Transportation...

  15. 77 FR 5876 - Notice of Meeting of the Transit Rail Advisory Committee for Safety (TRACS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-06

    ... Transit Administrator on matters relating to the safety of public transportation systems. DATES: The TRACS... Secretary and the Federal Transit Administrator on matters pertaining to the safety of public transportation... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF...

  16. 78 FR 28285 - Notice of Meeting of the Transit Rail Advisory Committee for Safety (TRACS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-14

    ... Transit Administrator on matters relating to the safety of public transportation systems. DATES: The TRACS... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION... public meeting of the Transit Rail Advisory Committee for Safety (TRACS). TRACS is a Federal...

  17. 75 FR 9343 - Nomenclature Change Relating to the Network Distribution Center Transition

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ... 111 and 121 Nomenclature Change Relating to the Network Distribution Center Transition AGENCY: Postal... to the ongoing transition of USPS bulk mail centers (BMC) to network distribution centers (NDC), by... Gambhir at 202-268-6256. SUPPLEMENTARY INFORMATION: Background: The BMC network was established in...

  18. 78 FR 27284 - Public Transportation on Indian Reservations Program; Tribal Transit Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ...This Notice accomplishes several purposes. First, the U.S. Department of Transportation (DOT), Federal Transit Administration (FTA) summarizes and responds to written comments FTA received in response to a November 9, 2012, Federal Register Notice regarding proposed grant program provisions for this modified program. Second, this Notice establishes the framework for the Tribal Transit Program,......

  19. 75 FR 67751 - Medicare Program: Community-Based Care Transitions Program (CCTP) Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-03

    ... HUMAN SERVICES Centers for Medicare & Medicaid Services Medicare Program: Community-Based Care... about the upcoming Community-based Care Transitions Program. The meeting is open to the public, but... will be posted on the CMS Care Transitions Web site at...

  20. In Vitro and In Vivo Biology of Recombinant Adenovirus Vectors with E1, E1/E2A, or E1/E4 Deleted

    PubMed Central

    Lusky, M.; Christ, M.; Rittner, K.; Dieterle, A.; Dreyer, D.; Mourot, B.; Schultz, H.; Stoeckel, F.; Pavirani, A.; Mehtali, M.

    1998-01-01

    Isogenic, E3-deleted adenovirus vectors defective in E1, E1 and E2A, or E1 and E4 were generated in complementation cell lines expressing E1, E1 and E2A, or E1 and E4 and characterized in vitro and in vivo. In the absence of complementation, deletion of both E1 and E2A completely abolished expression of early and late viral genes, while deletion of E1 and E4 impaired expression of viral genes, although at a lower level than the E1/E2A deletion. The in vivo persistence of these three types of vectors was monitored in selected strains of mice with viral genomes devoid of transgenes to exclude any interference by immunogenic transgene-encoded products. Our studies showed no significant differences among the vectors in the short-term maintenance and long-term (4-month) persistence of viral DNA in liver and lung cells of immunocompetent and immunodeficient mice. Furthermore, all vectors induced similar antibody responses and comparable levels of adenovirus-specific cytotoxic T lymphocytes. These results suggest that in the absence of transgenes, the progressive deletion of the adenovirus genome does not extend the in vivo persistence of the transduced cells and does not reduce the antivirus immune response. In addition, our data confirm that, in the absence of transgene expression, mouse cellular immunity to viral antigens plays a minor role in the progressive elimination of the virus genome. PMID:9499056

  1. 26 CFR 1.167(e)-1 - Change in method.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... to December 30, 2003 (§ 1.167(e)-1 as contained in 26 CFR part 1 edition revised as of April 1, 2003). ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Change in method. 1.167(e)-1 Section 1.167(e)-1...) INCOME TAXES (CONTINUED) Itemized Deductions for Individuals and Corporations § 1.167(e)-1 Change...

  2. 75 FR 37771 - Office of Postsecondary Education; Overview Information; Transition Programs for Students with...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-30

    ... Intellectual Disabilities Into Higher Education (TPSID)--Model Comprehensive Transition and Postsecondary Programs for Students With Intellectual Disabilities; Notice Inviting Applications for New Awards for... and postsecondary programs for students with intellectual disabilities. Priorities: This...

  3. 78 FR 69524 - Preparation of an Environmental Impact Statement for High Capacity Transit Improvements for the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-19

    ... Indianapolis Metropolitan Planning Organization (Indianapolis MPO) and Indianapolis Public Transportation... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION... Counties of Marion and Hamilton AGENCY: Federal Transit Administration, U.S. Department of...

  4. Enhanced spin-dependent parity-nonconservation effect in the 7 s 1/2 2S →6 d 5/2 2D transition in Fr: A possibility for unambiguous detection of the nuclear anapole moment

    NASA Astrophysics Data System (ADS)

    Sahoo, B. K.; Aoki, T.; Das, B. P.; Sakemi, Y.

    2016-03-01

    Employing the relativistic coupled-cluster method, comparative studies of the parity nonconserving electric dipole amplitudes for the 7 s 1/2 2S →6 d 5/2 2D transitions in 210Fr and 211Fr isotopes have been carried out. It is found that these transition amplitudes, sensitive only to the nuclear spin-dependent effects, are enhanced substantially owing to the very large contributions from the electron core-polarization effects in Fr. This translates to a relatively large and, in principle, measurable induced light shift, which would be a signature of nuclear spin-dependent parity nonconservation that is dominated by the nuclear anapole moment in a heavy atom like Fr. A plausible scheme to measure this quantity using the Cyclotron and Radioisotope Center (CYRIC) facility at Tohoku University has been outlined.

  5. CYP2E1 and Oxidative Liver Injury by Alcohol

    PubMed Central

    Lu, Yongke; Cederbaum, Arthur I.

    2008-01-01

    Ethanol-induced oxidative stress appears to play a major role in mechanisms by which ethanol causes liver injury. Many pathways have been suggested to contribute to the ability of ethanol to induce a state of oxidative stress. One central pathway appears to be the induction of cytochrome P450 2E1 (CYP2E1) by ethanol. CYP2E1 metabolizes and activates many toxicological substrates, including ethanol, to more reactive, toxic products. Levels of CYP2E1 are elevated under a variety of physiological and pathophysiological conditions, and after acute and chronic alcohol treatment. CYP2E1 is also an effective generator of reactive oxygen species such as the superoxide anion radical and hydrogen peroxide, and in the presence of iron catalysts, produces powerful oxidants such as the hydroxyl radical. This Review Article summarizes some of the biochemical and toxicological properties of CYP2E1, and briefly describes the use of cell lines developed to constitutively express CYP2E1 in assessing the actions of CYP2E1. Possible therapeutic implications for treatment of alcoholic liver injury by inhibition of CYP2E1 or CYP2E1-dependent oxidative stress will be discussed, followed by some future directions which may help to understand the actions of CYP2E1 and its role in alcoholic liver injury. PMID:18078827

  6. Activation of adenovirus 5 E1A transcription by region E1B in transformed primary rat cells.

    PubMed Central

    Jochemsen, A G; Peltenburg, L T; te Pas, M F; de Wit, C M; Bos, J L; van der Eb, A J

    1987-01-01

    The human adenovirus 5 E1A region can immortalize primary cultures of baby rat kidney cells, but requires the presence of the E1B region for complete oncogenic transformation. One of the effects of the E1B region in the transformation process is the activation of E1A expression. We have investigated the mechanism of this stimulation of E1A expression using nuclear run-on assays with nuclei from Ad5 E1A- and Ad5 E1-transformed cells. It was found that E1B enhances E1A at the level of transcription-initiation. This activation is mainly observed when the E1A and E1B regions are integrated simultaneously into the cellular genome and only minimally when these genes are integrated separately, strongly suggesting that a close physical linkage of these regions is essential for the observed effect. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 5. Fig. 6. PMID:2962857

  7. Activation of NF-κB by Human Papillomavirus 16 E1 Limits E1-Dependent Viral Replication through Degradation of E1

    PubMed Central

    Nakahara, Tomomi; Tanaka, Katsuyuki; Ohno, Shin-ichi; Egawa, Nagayasu; Yugawa, Takashi

    2015-01-01

    ABSTRACT NF-κB is a family of transcription factors that regulate gene expression involved in many processes, such as the inflammatory response and cancer progression. Little is known about associations of NF-κB with the human papillomavirus (HPV) life cycle. We have developed a tissue culture system to conditionally induce E1-dependent replication of the human papillomavirus 16 (HPV16) genome in human cervical keratinocytes and found that expression of HPV16 E1, a viral helicase, results in reduction of IκBα and subsequent activation of NF-κB in a manner dependent on helicase activity. Exogenous expression of a degradation-resistant mutant of IκBα, which inhibits the activation of NF-κB, enhanced E1-dependent replication of the viral genome. Wortmannin, a broad inhibitor of phosphoinositide 3-kinases (PI3Ks), and, to a lesser extent, VE-822, an ATR kinase inhibitor, but not KU55933, an ATM kinase inhibitor, suppressed the activation of NF-κB and augmented E1-dependent replication of the HPV16 genome. Interestingly, the enhancement of E1-dependent replication of the viral genome was associated with increased stability of E1 in the presence of wortmannin as well as the IκBα mutant. Collectively, we propose that expression of E1 induces NF-κB activation at least in part through the ATR-dependent DNA damage response and that NF-κB in turn limits E1-dependent replication of HPV16 through degradation of E1, so that E1 and NF-κB may constitute a negative feedback loop. IMPORTANCE A major risk factor in human papillomavirus (HPV)-associated cancers is persistent infection with high-risk HPVs. To eradicate viruses from infected tissue, it is important to understand molecular mechanisms underlying the establishment and maintenance of persistent infection. In this study, we obtained evidence that human papillomavirus 16 (HPV16) E1, a viral DNA helicase essential for amplification of the viral genomes, induces NF-κB activation and that this limits E1-dependent

  8. Optimization of Acidothermus Celluloyticus Endoglucanase (E1) Production in Transgenic Tobacco Plants by Transcriptional, Post-transcription and Post-Translational Modification

    SciTech Connect

    Dai, Ziyu; Hooker, Brian S.; Quesenberry, Ryan D.; Thomas, S. R.

    2005-10-01

    Biochemical characteristics of Acidothermus cellulolyticus endoglucanase (E1) and its physiological effects in transgenic tobacco (Nicotiana tabacum) has been studied previously. In an attempt to obtain a high level of production of intact E1 in transgenic plants, the E1 gene was expressed under the control of strong Mac promoter (a hybrid promoter of manopine synthase promoter and cauliflower mosaic virus 35S promoter enhancer region) or tomato Rubisco small subunit (RbcS-3C) promoter with different 5’ untranslated leader (UTL) sequence and targeted to different subcellular comartmentations with various transit peptides. The expression of E1 protein in transgenic tobacco plants was determined via E1 activity, protein immunobloting, and RNA gel-blotting analyses. Effects of different transit peptides on E1 protein production and its stability were examined in transgenic tobacco plants carrying one of six transgene expression vectors with the same (Mac) promoter and transcription terminator (Tmas). Transgenic tobacco plants with apoplast transit peptide (Mm-apo) had the highest average E1 activity and protein accumulation , while E1 protein was more stable in transgenic plants with no transit peptide (Mm) than others. The E1 expression under tomato RbcS-3C promoter was higher than that under Mac promoter based on the average E1 activity, E1 protein accumulation, and RNA gel-blotting. The E1 expression was increased more than two fold when the 5’-UTL of alfalfa mosaic virus RNA4 gene replaced the UTL of RbcS-3C promoter, while the UTL of alfalfa mosaic virus RNA4 gene was less effective than the UTL of Mac promoter. The optimal combination of promoter, 5’-UTL, and subcellular compartmentation (transit peptide) for E1 protein production in transgenic tobacco plants are discussed.

  9. 76 FR 11433 - Federal Transition To Secure Hash Algorithm (SHA)-256

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ...The Civilian Agency Acquisition Council, and the Defense Acquisition Regulations Council (Councils), are hosting the first of at least two public meetings to start a dialogue with industry and Government agencies about ways for the acquisition community to transition to Secure Hash Algorithm SHA-256. SHA-256 is a cryptographic hash function that is used in digital signatures, and......

  10. 75 FR 18942 - FY 2010 Discretionary Sustainability Funding Opportunity; Transit Investments for Greenhouse Gas...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... Investments for Greenhouse Gas and Energy Reduction (TIGGER) Program and Clean Fuels Grant Program, Augmented... clean energy sources that will both enhance the environment through improved air quality and curb our... funds in Fiscal Year (FY) 2010 for the Transit Investments for Greenhouse Gas and Energy...

  11. 75 FR 3956 - Intent To Prepare an Environmental Impact Statement for Proposed Transit Improvements in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-25

    ... system in Los Angeles County. The proposed project would provide a continuation of the Metro Gold Line... transportation and land use plans. Phase 2 would expand upon the Gold Line Eastside Extension investment and... provide the study area with improved fixed-guideway east-west transit service from the Metro Gold...

  12. 77 FR 41801 - Solicitation for a Cooperative Agreement-Transition From Jail to the Community (TJC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-16

    ... National Institute of Corrections Solicitation for a Cooperative Agreement--Transition From Jail to the... for a Cooperative Agreement. SUMMARY: The National Institute of Corrections (NIC) is soliciting proposals from organizations, groups, or individuals interested in entering into a 30-month...

  13. 78 FR 64245 - AG Survey of Transitional Housing Assistance for Victims of Domestic Violence, Dating Violence...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-28

    ... Survey of Transitional Housing Assistance for Victims of Domestic Violence, Dating Violence, Stalking, or... notice. The Department of Justice, Office on Violence Against Women (OVW) will be submitting the... collection. If you have questions concerning the collection, please Cathy Poston, Office on Violence...

  14. 76 FR 207 - Intent To Prepare an Environmental Impact Statement for Proposed Transit Improvements to the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-03

    ... Improvements to the North Red and Purple Lines, Cook County, IL AGENCY: Federal Transit Administration, U.S... Authority (CTA) intend to prepare a Tier 1 Environmental Impact Statement (Tier 1 EIS) for the North Red and... North Red and Purple lines up to a state of good repair from the track structure immediately north...

  15. 75 FR 51523 - Notice of Meeting of the Transit Rail Advisory Committee for Safety (TRACS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-20

    ... Transit Administrator on matters relating to the safety of public transportation systems. DATES: The TRACS... pertaining to the safety of public transportation systems. TRACS is composed of 21 members representing a... Department of Transportation Richard W. Clark, California Public Utilities Commission Diane Davidson,...

  16. 76 FR 16854 - Notice of Meeting of the Transit Rail Advisory Committee for Safety (TRACS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ... Administrator on matters relating to the safety of public transportation systems. DATES: The TRACS meeting will... of the Federal Transit Administration on matters relating to the safety of public transportation... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF...

  17. 78 FR 67212 - Notice of Meeting of the Transit Rail Advisory Committee for Safety (TRACS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-08

    ... Secretary and the Federal Transit Administrator on matters relating to the safety of public transportation... (FTA) on matters relating to the safety of public transportation systems. TRACS is currently composed... National Public Transportation Safety Plan (4) Review of the Draft Letter Report on Public...

  18. 76 FR 75953 - Homeless Emergency Assistance and Rapid Transition to Housing: Emergency Solutions Grants Program...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    ...The Homeless Emergency Assistance and Rapid Transition to Housing Act of 2009 (HEARTH Act), enacted into law on May 20, 2009, consolidates three of the separate homeless assistance programs administered by HUD under the McKinney-Vento Homeless Assistance Act into a single grant program, and revises the Emergency Shelter Grants program and renames it as the Emergency Solutions Grants (ESG)......

  19. 75 FR 10799 - Transitions Optical, Inc.; Analysis to Aid Public Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-09

    ... Transitions Optical, Inc.; Analysis to Aid Public Comment AGENCY: Federal Trade Commission. ACTION: Proposed... prohibiting unfair or deceptive acts or practices or unfair methods of competition. The attached Analysis to... public record for a period of thirty (30) days. The following Analysis to Aid Public Comment...

  20. 75 FR 67019 - Presidential Determination on Major Illicit Drug Transit or Major Illicit Drug Producing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-01

    ... From the Federal Register Online via the Government Publishing Office #0; #0; #0; Presidential Documents #0; #0; #0;#0;Federal Register / Vol. 75 , No. 210 / Monday, November 1, 2010 / Presidential Documents#0;#0; ] Presidential Determination No. 2011-16 of September 15, 2010 Presidential Determination on Major Illicit Drug Transit or Major Illicit Drug...

  1. 76 FR 71464 - Defense Federal Acquisition Regulation Supplement; Transition to the System for Award Management...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ... Integrated Acquisition Environment systems to the new System for Award Management architecture. DATES... the IAE to the new System for Award Management (SAM) architecture has begun. Phase One will transition... Representations and Certifications Application (ORCA) to the new SAM architecture. This rule provides the...

  2. 77 FR 47692 - Notice of Transportation Services' Transition From Paper to Electronic Fare Media Comments...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-09

    ...On March 29, 2011, and April 2, 2012, U.S. Department of Transportation's Office of Transportation Services (TRANServe), located within the Office of the Assistant Secretary for Administration, published for public comment Notices of the adoption of a new program distribution methodology for transit benefits. To date, TRANServe has implemented its plan in three of the eight TRANServe Services......

  3. 77 FR 55813 - Transition of DOE-ID Public Reading Room

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Transition of DOE-ID Public Reading Room AGENCY: Idaho Operations Office, Department of Energy. ACTION... Department of Energy Idaho Operations Office Public Reading Room has been relocated to the INL...

  4. 76 FR 31354 - Notice of Issuance of Final Determination Concerning the Transit Connect Electric Vehicle

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... and Border Protection (``CBP'') has issued a final determination concerning the country of origin of... determination that the United States is the country of origin of the vehicle for purposes of U.S. Government... final determination concerning the country of origin of the Transit Connect Electric Vehicle which...

  5. Evidence for the onset of reflection asymmetry in sup 216 Fr

    SciTech Connect

    Debray, M.E.; Davidson, J.; Davidson, M.; Kreiner, A.J.; Hojman, D.; Santos, D. ); Ahn, K.; Fossan, D.B.; Liang, Y.; Ma, R.; Paul, E.S.; Piel, W.F. Jr.; Xu, N. )

    1990-05-01

    States in doubly odd {sup 216}Fr have been studied using in-beam {alpha}, {gamma}, and {ital e}{sup {minus}} spectroscopy techniques through the {sup 208}Pb({sup 11}B3{ital n}) fusion-evaporation reaction. {sup 216}Fr shows a band structure with interleaved states of alternating parities connected by enhanced {ital B}({ital E}1) transitions. It represents the lowest-mass corner of the region ({ital Z}{ge}87,{ital N}{ge}129) in which this phenomenon is observed.

  6. The nuclear receptor NR2E1/TLX controls senescence

    PubMed Central

    Krusche, Benjamin; Pemberton, Helen; Alonso, Marta M.; Chandler, Hollie; Brookes, Sharon; Parrinello, Simona; Peters, Gordon; Gil, Jesús

    2014-01-01

    The nuclear receptor NR2E1 (also known as TLX or tailless) controls the self-renewal of neural stem cells (NSCs) and has been implied as an oncogene which initiates brain tumours including glioblastomas. Despite NR2E1 regulating targets like p21CIP1 or PTEN we still lack a full explanation for its role in NSC self-renewal and tumorigenesis. We know that Polycomb repressive complexes (PRC) also control stem cell self-renewal and tumorigenesis, but so far, no formal connection has been established between NR2E1 and PRCs. In a screen for transcription factors regulating the expression of the Polycomb protein CBX7, we identified NR2E1 as one of its more prominent regulators. NR2E1 binds at the CBX7 promoter, inducing its expression. Notably CBX7 represses NR2E1 as part of a regulatory loop. Ectopic NR2E1 expression inhibits cellular senescence, extending cellular lifespan in fibroblasts via CBX7-mediated regulation of p16INK4a and direct repression of p21CIP1. In addition NR2E1 expression also counteracts oncogene-induced senescence (OIS). The importance of NR2E1 to restrain senescence is highlighted through the process of knocking down its expression, which causes premature senescence in human fibroblasts and epithelial cells. We also confirmed that NR2E1 regulates CBX7 and restrains senescence in NSCs. Finally, we observed that the expression of NR2E1 directly correlates with that of CBX7 in human glioblastoma multiforme. Overall we identified control of senescence and regulation of Polycomb action as two possible mechanisms that can join those so far invoked to explain the role of NR2E1 in control of NSC self-renewal and cancer. PMID:25328137

  7. Calculation of Radiative Corrections to E1 matrix elements in the Neutral Alkalis

    SciTech Connect

    Sapirstein, J; Cheng, K T

    2004-09-28

    Radiative corrections to E1 matrix elements for ns-np transitions in the alkali metal atoms lithium through francium are evaluated. They are found to be small for the lighter alkalis but significantly larger for the heavier alkalis, and in the case of cesium much larger than the experimental accuracy. The relation of the matrix element calculation to a recent decay rate calculation for hydrogenic ions is discussed, and application of the method to parity nonconservation in cesium is described.

  8. The multiple roles of cyclin E1 in controlling cell cycle progression and cellular morphology of Trypanosoma brucei.

    PubMed

    Gourguechon, Stéphane; Savich, Jason M; Wang, Ching C

    2007-05-11

    Regulation of eukaryotic cell cycle progression requires sequential activation and inactivation of cyclin-dependent kinases. Previous RNA interference (RNAi) experiments in Trypanosoma brucei indicated that cyclin E1, cdc2-related kinase (CRK)1 and CRK2 are involved in regulating G1/S transition, whereas cyclin B2 and CRK3 play a pivotal role in controlling the G2/M checkpoint. To search for potential interactions between the other cyclins and CRKs that may not have been revealed by the RNAi assays, we used the yeast two-hybrid system and an in vitro glutathione-S-transferase pulldown assay and observed interactions between cyclin E1 and CRK1, CRK2 and CRK3. Cyclins E1-E4 are homologues of yeast Pho80 cyclin. But yeast complementation assays indicated that none of them possesses a Pho80-like function. Analysis of cyclin E1+CRK1 and cyclin E1+CRK2 double knockdowns in the procyclic form of T. brucei indicated that the cells were arrested more extensively in the G1 phase beyond the cumulative effect of individual knockdowns. But BrdU incorporation was impaired significantly only in cyclin E1+CRK1-depleted cells, whereas a higher percentage of cyclin E1+CRK2 knockdown cells assumed a grossly elongated posterior end morphology. A double knockdown of cyclin E1 and CRK3 arrested cells in G2/M much more efficiently than if only CRK3 was depleted. Taken together, these data suggest multiple functions of cyclin E1: it forms a complex with CRK1 in promoting G1/S phase transition; it forms a complex with CRK2 in controlling the posterior morphogenesis during G1/S transition; and it forms a complex with CRK3 in promoting passage across the G2/M checkpoint in the trypanosome. PMID:17376478

  9. THE MULTIPLE ROLES OF CYCLIN E1 IN CONTROLLING CELL CYCLE PROGRESSION AND CELLULAR MORPHOLOGY OF TRYPANOSOMA BRUCEI

    PubMed Central

    Gourguechon, Stéphane; Savich, Jason M.; Wang, Ching C.

    2009-01-01

    SUMMARY Regulation of eukaryotic cell cycle progression requires sequential activation and inactivation of cyclin-dependent kinases. Previous RNA interference (RNAi) experiments in Trypanosoma brucei indicated that cyclin E1, cdc2-related kinase (CRK)1 and CRK2 are involved in regulating G1/S transition, whereas cyclin B2 and CRK3 play a pivotal role in controlling the G2/M checkpoint. To search for potential interactions between the other cyclins and CRKs that may not have been revealed by the RNAi assays, we used the yeast two-hybrid system and an in vitro GST pulldown assay and observed interactions between cyclin E1 and CRK1, CRK2 and CRK3. Cyclins E1-E4 are homologues of yeast Pho80 cyclin. But yeast complementation assays indicated that none of them possesses a Pho80-like function. Analysis of cyclin E1+CRK1 and cyclin E1+CRK2 double knockdowns in the procyclic form of T. brucei indicated that the cells were arrested more extensively in the G1 phase beyond the cumulative effect of individual knockdowns. But BrdU incorporation was significantly impaired only in cyclin E1+CRK1 depleted cells, whereas a higher percentage of cyclin E1+CRK2 knockdown cells assumed a grossly elongated posterior end morphology. A double knockdown of cyclin E1 and CRK3 arrested cells in G2/M much more efficiently than if CRK3 was depleted alone. Taken together, these data suggest multiple functions of cyclin E1. It forms a complex with CRK1 in promoting G1/S phase transition, with CRK2 in controlling the posterior morphogenesis during G1/S transition and with CRK3 in promoting passage across the G2/M checkpoint in the trypanosome. PMID:17376478

  10. Chemical characteristics for optimizing CYP2E1 inhibition.

    PubMed

    van de Wier, B; Balk, J M; Bast, A; Koek, G H; Haenen, G R M M

    2015-12-01

    Cytochrome P450 2E1 (CYP2E1) expression and activity in the liver is associated with the degree of liver damage in patients with alcoholic steatohepatitis (ASH) as well as non-alcoholic steatohepatitis (NASH). CYP2E1 is known to generate reactive oxygen species, which leads to oxidative stress, one of the hallmarks of both diseases. Apart from ROS, toxic metabolites can be formed by CYP2E1 metabolism, further potentiating liver injury. Therefore, CYP2E1 is implicated in the pathogenesis of ASH and NASH. The aim of this study was to determine the chemical characteristics of compounds that are important to inhibit CYP2E1. To this end, structurally related analogs that differed in their lipophilic, steric and electronic properties were tested. In addition, homologues series of aliphatic primary alcohols, secondary alcohols, aldehydes, ketones and carboxylic acids were tested. It was found that inhibition of the CYP2E1 activity is primarily governed by lipophilicity. The optimal log D7.4 (octanol/water distribution coefficient at pH 7.4) value for inhibition of CYP2E1 was approximately 2.4. In the carboxylic acids series the interaction of the carboxylate group with polar residues lining the CYP2E1 active site also has to be considered. This study sketches the basic prerequisites in the search for inhibitors of CYP2E1, which would strengthen our therapeutic armamentarium against CYP2E1 associated diseases, such as ASH and NASH. PMID:26428356

  11. 26 CFR 1.927(e)-1 - Special sourcing rule.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Earned Income of Citizens of United States § 1.927(e)-1 Special sourcing rule. (a) Source rules for related persons—(1) In general. The income of a person described in...

  12. 26 CFR 1.927(e)-1 - Special sourcing rule.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Earned Income of Citizens of United States § 1.927(e)-1 Special sourcing rule. (a) Source rules for related persons—(1) In general. The income of a person described in section 482 from...

  13. 26 CFR 1.665(e)-1 - Preceding taxable year.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Preceding taxable year. 1.665(e)-1 Section 1.665... (CONTINUED) INCOME TAXES Treatment of Excess Distributions of Trusts Applicable to Taxable Years Beginning Before January 1, 1969 § 1.665(e)-1 Preceding taxable year. (a) Definition. For purposes of subpart...

  14. 26 CFR 1.665(e)-1 - Preceding taxable year.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Preceding taxable year. 1.665(e)-1 Section 1.665... (CONTINUED) INCOME TAXES (CONTINUED) Treatment of Excess Distributions of Trusts Applicable to Taxable Years Beginning Before January 1, 1969 § 1.665(e)-1 Preceding taxable year. (a) Definition. For purposes...

  15. 26 CFR 1.642(e)-1 - Depreciation and depletion.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Depreciation and depletion. 1.642(e)-1 Section 1... (CONTINUED) INCOME TAXES Estates, Trusts, and Beneficiaries § 1.642(e)-1 Depreciation and depletion. An estate or trust is allowed the deductions for depreciation and depletion, but only to the extent...

  16. 26 CFR 1.514(e)-1 - Allocation rules.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Allocation rules. 1.514(e)-1 Section 1.514(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Taxation of Business Income of Certain Exempt Organizations §...

  17. 26 CFR 1.665(e)-1 - Preceding taxable year.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 8 2012-04-01 2012-04-01 false Preceding taxable year. 1.665(e)-1 Section 1.665... (CONTINUED) INCOME TAXES (CONTINUED) Treatment of Excess Distributions of Trusts Applicable to Taxable Years Beginning Before January 1, 1969 § 1.665(e)-1 Preceding taxable year. (a) Definition. For purposes...

  18. 26 CFR 1.665(e)-1 - Preceding taxable year.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 8 2014-04-01 2014-04-01 false Preceding taxable year. 1.665(e)-1 Section 1.665... (CONTINUED) INCOME TAXES (CONTINUED) Treatment of Excess Distributions of Trusts Applicable to Taxable Years Beginning Before January 1, 1969 § 1.665(e)-1 Preceding taxable year. (a) Definition. For purposes...

  19. 78 FR 49600 - Notice of Intent To Prepare an Environmental Impact Statement for the Virginia Beach Transit...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-14

    ...The Federal Transit Administration (FTA) and Hampton Roads Transit (HRT) are planning to prepare an Environmental Impact Statement (EIS) for the Virginia Beach Transit Extension Study (VBTES). The VBTES will examine extending transit service from the eastern terminus of Norfolk's existing Light Rail Transit (LRT) system, ``The Tide,'' at Newtown Road to the Virginia Beach Oceanfront either......

  20. Resolvin E1 Regulates ADP Activation of Human Platelets

    PubMed Central

    Fredman, Gabrielle; Van Dyke, Thomas E.; Serhan, Charles N.

    2010-01-01

    Objective Resolvin E1 (RvE1) is an eicosapentaenoic acid (EPA)-derived specialized pro-resolving mediator generated during resolution of acute inflammation. RvE1 exhibits potent organ-protective actions in vivo and acts on specific cell types including platelets. Here, we investigated the ability of RvE1 to regulate adenosine diphosphate (ADP) activation of platelets via specific receptors because RvE1 reduces platelet aggregation with certain agonists including ADP. Methods and Results RvE1 (0.1nM–100nM) incubated with platelets gave reduced ADP-stimulated P-selectin mobilization (IC50 ~1.6×10−12 M) and polymerized actin content compared to control platelets. RvE1 (1–100nM) did not stimulate or block intracellular calcium mobilization. Using a new P2Y12-β-arrestin-coupled cell system, ADP-activated P2Y12 with an EC50 of 5×10−6 M and RvE1 did not directly stimulate P2Y12 or block ADP-P2Y12 signals. In this system, another eicosanoid LTE4 (EC50 1.3×10−11 M) dose dependently activated P2Y12. When recombinant P2Y12-expressing cells were transiently transfected with an RvE1 receptor, human ChemR23 (present on human platelets), addition of RvE1 (0.1nM-10.0nM) blocked ADP signals (IC50 ~1.6×10−11 M) in P2Y12-ChemR23-expressing cells compared to mock transfections. Conclusions These results demonstrate that RvE1’s regulatory actions (i.e reducing ADP-stimulated P-selectin mobilization and actin polymerization) are hChemR23-dependent. Moreover, they document specific platelet actions of RvE1 selectively engaged with ADP-activated platelets that illuminate a new cellular mechanism and impact of omega-3 EPA that may contribute to both resolution of vascular inflammation and ADP-dependent platelet activation relevant in pathologic cardiovascular events. PMID:20702811

  1. Molecular basis of maple syrup urine disease: Novel mutations at the E1[alpha] locus that impair E1([alpha][sub 2][beta][sub 2]) assembly or decrease steady-state E1[alpha] mRNA levels of branched-chain [alpha]-keto acid dehydrogenase complex

    SciTech Connect

    Chuang, J.L.; Fisher, C.R.; Chuang, D.T.; Cox, R.P. )

    1994-08-01

    The authors report the occurrence of three novel mutations in the E1[alpha] (BCKDHA) locus of the branched-chain [alpha]-keto acid dehydrogenase (BCKAD) complex that cause maple syrup urine disease (MSUD). An 8-bp deletion in exon 7 is present in one allele of a compound-heterozygous patient (GM-649). A single C nucleotide insertion in exon 2 occurs in one allele of an intermediate-MSUD patient (Lo). The second allele of patient Lo carries an A-to-G transition in exon 9 of the E1[alpha] gene. This missense mutation changes Tyr-368 to Cys (Y368C) in the E1[alpha] subunit. Both the 8-bp deletion and the single C insertion generate a downstream nonsense codon. Both mutations appear to be associated with a low abundance of the mutant E1[alpha] mRNA, as determined by allele-specific oligonucleotide probing. Transfection studies strongly suggest that the Y368C substitution in the E1[alpha] subunit impairs its proper assembly with the normal E1[beta]. Unassembled as well as misassembled E1[alpha] and E1[beta] subunits are degraded in the cell. 32 refs., 8 figs.

  2. Establishment of Class e1 Mass Standard of 50 kg

    NASA Astrophysics Data System (ADS)

    Yao, Hong; Wang, Jian; Ding, Jingan; Zhong, Ruilin; Ren, Xiaoping

    Because of the equipment limit, the dissemination of large mass has been realized by a large amount of higher class of 20 kg weights since 1950s in China. But with improvement of the technique and customer's requirements, it is necessary to establish the mass standard of 50 kg weight. In 1990s, mass standard laboratory has set up Class E1 weight sets from 20 kg to 1 mg. To extend the mass capacity up to 50 kg of Class E1, it is not only to produce Class E1 50 kg weight and import a mass comparator, but also need to lift the heavy weight from weight box to balance receptor safely. Up to now, the mass comparator has been installed in Hepingli campus of NIM. Two pieces of Class E1 50 kg weights are determined by combination weighing method. A lifting device has been mounted close to the mass comparator in order to move the 50 kg easily.

  3. Influence of neutron surface on E1 resonance properties

    NASA Astrophysics Data System (ADS)

    Goncharova, N. G.; Tretyakova, T. Yu.; Fedorov, N. A.

    2016-01-01

    The E1 strength distributions in even-even Si isotopes were calculated in the "particle-core coupling" version of the shell model taking into account the fragmentation of the hole configuration among the states of the daughter nuclei. The comparison of calculated strength distributions in different isotopes of the same element shows the peculiarities of a neutron surface influence on the E1 resonance fragmentation.

  4. Oncolytic Replication of E1b-Deleted Adenoviruses

    PubMed Central

    Cheng, Pei-Hsin; Wechman, Stephen L.; McMasters, Kelly M.; Zhou, Heshan Sam

    2015-01-01

    Various viruses have been studied and developed for oncolytic virotherapies. In virotherapy, a relatively small amount of viruses used in an intratumoral injection preferentially replicate in and lyse cancer cells, leading to the release of amplified viral particles that spread the infection to the surrounding tumor cells and reduce the tumor mass. Adenoviruses (Ads) are most commonly used for oncolytic virotherapy due to their infection efficacy, high titer production, safety, easy genetic modification, and well-studied replication characteristics. Ads with deletion of E1b55K preferentially replicate in and destroy cancer cells and have been used in multiple clinical trials. H101, one of the E1b55K-deleted Ads, has been used for the treatment of late-stage cancers as the first approved virotherapy agent. However, the mechanism of selective replication of E1b-deleted Ads in cancer cells is still not well characterized. This review will focus on three potential molecular mechanisms of oncolytic replication of E1b55K-deleted Ads. These mechanisms are based upon the functions of the viral E1B55K protein that are associated with p53 inhibition, late viral mRNA export, and cell cycle disruption. PMID:26561828

  5. Oncolytic Replication of E1b-Deleted Adenoviruses.

    PubMed

    Cheng, Pei-Hsin; Wechman, Stephen L; McMasters, Kelly M; Zhou, Heshan Sam

    2015-11-01

    Various viruses have been studied and developed for oncolytic virotherapies. In virotherapy, a relatively small amount of viruses used in an intratumoral injection preferentially replicate in and lyse cancer cells, leading to the release of amplified viral particles that spread the infection to the surrounding tumor cells and reduce the tumor mass. Adenoviruses (Ads) are most commonly used for oncolytic virotherapy due to their infection efficacy, high titer production, safety, easy genetic modification, and well-studied replication characteristics. Ads with deletion of E1b55K preferentially replicate in and destroy cancer cells and have been used in multiple clinical trials. H101, one of the E1b55K-deleted Ads, has been used for the treatment of late-stage cancers as the first approved virotherapy agent. However, the mechanism of selective replication of E1b-deleted Ads in cancer cells is still not well characterized. This review will focus on three potential molecular mechanisms of oncolytic replication of E1b55K-deleted Ads. These mechanisms are based upon the functions of the viral E1B55K protein that are associated with p53 inhibition, late viralmRNAexport, and cell cycle disruption. PMID:26561828

  6. Impotence evaluated by the use of prostaglandin E1

    SciTech Connect

    Hwang, T.I.; Yang, C.R.; Wang, S.J.; Chang, C.L.; Tzai, T.S.; Chang, C.H.; Wu, H.C.

    1989-06-01

    We screened 80 patients at our hospital for the differential diagnosis of impotence using intracavernous injection of prostaglandin E1 (20 micrograms). The rate of positive response was 78.8 per cent (63 patients). Neither systemic reactions nor priapism occurred. However, a considerable incidence (23.8 per cent, 19 of 80 patients) of tolerable injection pain was encountered. The 133-xenon penile washout study was conducted routinely in impotent men for hemodynamic evaluation of penile vascularity. In 80 patients a positive correlation between the response of intracavernous prostaglandin E1 injection and the result of the washout study was found (r equals 0.381, p less than 0.0002). We selected 14 subjects randomly to receive additional intravenous infusions of prostaglandin E1 (6 ampules, 120 micrograms total) for 3 days, after which another 133-xenon washout study was done. The washout studies before and after intravenous prostaglandin E1 infusion were compared, and 10 patients (71.4 per cent) appeared to obtain improvement in half-time clearance and penile blood flow. However, only 3 patients noticed improvement subjectively. We suggest that prostaglandin E1 could be a desirable alternative for the diagnosis and treatment of impotence.

  7. Optimization of Acidothermus cellulolyticus endoglucanase (E1) production in transgenic tobacco plants by transcriptional, post-transcription and post-translational modification.

    PubMed

    Dai, Ziyu; Hooker, Brian S; Quesenberry, Ryan D; Thomas, Steven R

    2005-10-01

    An attempt was made to obtain a high-level production of intact Acidothermus cellulolyticus endoglucanase (E1) in transgenic tobacco plants. The E1 expression was examined under the control of the constitutive and strong Mac promoter or light-inducible tomato Rubisco small sub-unit (RbcS-3C) promoter with its original or Alfalfa Mosaic Virus (AMV) RNA4 5'-untranslated leader (UTL) and targeted to different sub-cellular compartments via transit peptides. The transit peptides included native E1, endoplasmic reticulum, vacuole, apoplast, and chloroplast. E1 expression and its stability in transgenic plants were determined via E1 activity, protein immunoblotting, and RNA gel-blotting analyses. Effects of sub-cellular compartments on E1 production and its stability were determined in transgenic tobacco plants carrying one of six transgene expression vectors, where the E1 was under the control of Mac promoter, mannopine synthase transcription terminator, and one of the five transit peptides. Transgenic tobacco plants with an apoplastic transit peptide had the highest average E1 activity and protein accumulation, which was about 0.25% of total leaf soluble proteins estimated via E1 specific activity and protein gel blots. Intercellular fluid analyses confirmed that E1 signal peptide functioned properly in tobacco cells to secret E1 protein into the apoplast. By replacing RbcS-3C UTL with AMV RNA4 UTL E1 production was enhanced more than twofold, while it was less effective than the mannopine synthase UTL. It was observed that RbcS-3C promoter was more favorable for E1 expression in transgenic plants than the Mac promoter. E1 activity in dried tobacco seeds stored one year at room temperature was 45% higher than that observed immediately after harvesting, suggesting that E1 protein can be stored at room temperature for a long period. E1 stability in different sub-cellular compartments and the optimal combination of promoter, 5'-UTL, and sub-cellular compartmentation for

  8. The Soybean-Specific Maturity Gene E1 Family of Floral Repressors Controls Night-Break Responses through Down-Regulation of FLOWERING LOCUS T Orthologs.

    PubMed

    Xu, Meilan; Yamagishi, Noriko; Zhao, Chen; Takeshima, Ryoma; Kasai, Megumi; Watanabe, Satoshi; Kanazawa, Akira; Yoshikawa, Nobuyuki; Liu, Baohui; Yamada, Tetsuya; Abe, Jun

    2015-08-01

    Photoperiodism is a rhythmic change of sensitivity to light, which helps plants to adjust flowering time according to seasonal changes in daylength and to adapt to growing conditions at various latitudes. To reveal the molecular basis of photoperiodism in soybean (Glycine max), a facultative short-day plant, we analyzed the transcriptional profiles of the maturity gene E1 family and two FLOWERING LOCUS T (FT) orthologs (FT2a and FT5a). E1, a repressor for FT2a and FT5a, and its two homologs, E1-like-a (E1La) and E1Lb, exhibited two peaks of expression in long days. Using two different approaches (experiments with transition between light and dark phases and night-break experiments), we revealed that the E1 family genes were expressed only during light periods and that their induction after dawn in long days required a period of light before dusk the previous day. In the cultivar Toyomusume, which lacks the E1 gene, virus-induced silencing of E1La and E1Lb up-regulated the expression of FT2a and FT5a and led to early flowering. Therefore, E1, E1La, and E1Lb function similarly in flowering. Regulation of E1 and E1L expression by light was under the control of E3 and E4, which encode phytochrome A proteins. Our data suggest that phytochrome A-mediated transcriptional induction of E1 and its homologs by light plays a critical role in photoperiodic induction of flowering in soybean. PMID:26134161

  9. The Soybean-Specific Maturity Gene E1 Family of Floral Repressors Controls Night-Break Responses through Down-Regulation of FLOWERING LOCUS T Orthologs1[OPEN

    PubMed Central

    Xu, Meilan; Yamagishi, Noriko; Zhao, Chen; Takeshima, Ryoma; Kasai, Megumi; Watanabe, Satoshi; Kanazawa, Akira; Yoshikawa, Nobuyuki; Liu, Baohui; Yamada, Tetsuya; Abe, Jun

    2015-01-01

    Photoperiodism is a rhythmic change of sensitivity to light, which helps plants to adjust flowering time according to seasonal changes in daylength and to adapt to growing conditions at various latitudes. To reveal the molecular basis of photoperiodism in soybean (Glycine max), a facultative short-day plant, we analyzed the transcriptional profiles of the maturity gene E1 family and two FLOWERING LOCUS T (FT) orthologs (FT2a and FT5a). E1, a repressor for FT2a and FT5a, and its two homologs, E1-like-a (E1La) and E1Lb, exhibited two peaks of expression in long days. Using two different approaches (experiments with transition between light and dark phases and night-break experiments), we revealed that the E1 family genes were expressed only during light periods and that their induction after dawn in long days required a period of light before dusk the previous day. In the cultivar Toyomusume, which lacks the E1 gene, virus-induced silencing of E1La and E1Lb up-regulated the expression of FT2a and FT5a and led to early flowering. Therefore, E1, E1La, and E1Lb function similarly in flowering. Regulation of E1 and E1L expression by light was under the control of E3 and E4, which encode phytochrome A proteins. Our data suggest that phytochrome A-mediated transcriptional induction of E1 and its homologs by light plays a critical role in photoperiodic induction of flowering in soybean. PMID:26134161

  10. Acetaldehyde and parkinsonism: role of CYP450 2E1

    PubMed Central

    Vaglini, Francesca; Viaggi, Cristina; Piro, Valentina; Pardini, Carla; Gerace, Claudio; Scarselli, Marco; Corsini, Giovanni Umberto

    2013-01-01

    The present review update the relationship between acetaldehyde (ACE) and parkinsonism with a specific focus on the role of P450 system and CYP 2E1 isozyme particularly. We have indicated that ACE is able to enhance the parkinsonism induced in mice by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, a neurotoxin able to damage the nigrostriatal dopaminergic pathway. Similarly diethyldithiocarbamate, the main metabolite of disulfiram, a drug widely used to control alcoholism, diallylsulfide (DAS) and phenylisothiocyanate also markedly enhance the toxin-related parkinsonism. All these compounds are substrate/inhibitors of CYP450 2E1 isozyme. The presence of CYP 2E1 has been detected in the dopamine (DA) neurons of rodent Substantia Nigra (SN), but a precise function of the enzyme has not been elucidated yet. By treating CYP 2E1 knockout (KO) mice with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, the SN induced lesion was significantly reduced when compared with the lesion observed in wild-type animals. Several in vivo and in vitro studies led to the conclusion that CYP 2E1 may enhance the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine toxicity in mice by increasing free radical production inside the dopaminergic neurons. ACE is a good substrate for CYP 2E1 enzyme as the other substrate-inhibitors and by this way may facilitate the susceptibility of dopaminergic neurons to toxic events. The literature suggests that ethanol and/or disulfiram may be responsible for toxic parkinsonism in human and it indicates that basal ganglia are the major targets of disulfiram toxicity. A very recent study reports that there are a decreased methylation of the CYP 2E1 gene and increased expression of CYP 2E1 mRNA in Parkinson's disease (PD) patient brains. This study suggests that epigenetic variants of this cytochrome contribute to the susceptibility, thus confirming multiples lines of evidence which indicate a link between environmental toxins and PD. PMID:23801948

  11. Calculation of radiative corrections to E1 matrix elements in the neutral alkali metals

    SciTech Connect

    Sapirstein, J.; Cheng, K.T.

    2005-02-01

    Radiative corrections to E1 matrix elements for ns-np transitions in the alkali-metal atoms lithium through francium are evaluated. They are found to be small for the lighter alkali metals but significantly larger for the heavier alkali metals, and in the case of cesium much larger than the experimental accuracy. The relation of the matrix element calculation to a recent decay rate calculation for hydrogenic ions is discussed, and application of the method to parity nonconservation in cesium is described.

  12. The emission spectroscopy of the e1Π-a1Δ system of VN molecule

    NASA Astrophysics Data System (ADS)

    Fan, Qunchao; Hu, Shi; Sun, Weiguo; Wang, Qi

    2013-10-01

    Vanadium nitride is an important metallurgical additive and a good electrode material molecule. It is necessary to understand its intrinsic microstructure for better applications. Four groups of known experimental transition data of low-lying rotational quantum states and the analytical formula developed recently by Sun group are used to study the Ree, Pee, Rff and Pff-branch emission spectra of the (0, 0) band of the e1Π-a1Δ system of VN molecule respectively. The results not only reproduce all known experimental spectral lines accurately, but also generate valid data of the unknown spectral lines up to J = 80 that may not be available experimentally.

  13. 77 FR 31337 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-25

    ... of the Secretary Department of Defense Task Force on the Care, Management, and Transition of... the Care, Management, and Transition of Recovering Wounded, Ill, and Injured Members of the Armed... Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured...

  14. 76 FR 71331 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-17

    ... of the Secretary Department of Defense Task Force on the Care, Management, and Transition of... on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured Members of the Armed... Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill,...

  15. 76 FR 38118 - Meeting of the Department of Defense Task Force on the Care, Management, and Transition of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-29

    ... Transition of Recovering Wounded, Ill, and Injured Members of the Armed Forces AGENCY: Department of Defense... Committee meeting of the Department of Defense Task Force on the Care, Management, and Transition of... Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured...

  16. 75 FR 45008 - Intent To Prepare an Environmental Impact Statement for a Proposed Light Rail Transit Line in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-30

    ... Federal Transit Administration Intent To Prepare an Environmental Impact Statement for a Proposed Light... Impact Statement (EIS) for the proposed Woodward Avenue Light Rail Transit (LRT) project in Detroit... selected Woodward Avenue as the Locally Preferred Alternative (LPA) with Light Rail Transit as...

  17. 78 FR 38796 - Intent To Prepare an Environmental Impact Statement for Increased Transit Service to King of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-27

    ... Federal Transit Administration Intent To Prepare an Environmental Impact Statement for Increased Transit... (NOI) to prepare an Environmental Impact Statement and Section 4(f) Evaluation. SUMMARY: The FTA and... Environmental Impact Statement (EIS) and Section 4(f) Evaluation for increased transit service to King...

  18. 76 FR 1501 - Preparation of Environmental Impact Statement for Transit Improvements in the US 90A/Southwest...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ...The Federal Transit Administration (FTA) and the Metropolitan Transit Authority of Harris County (METRO) intend to prepare an Environmental Impact Statement (EIS) in accordance with the National Environmental Policy Act (NEPA), to evaluate the proposed transit improvements in the US 90A/Southwest Rail corridor in the Houston metropolitan area (Harris County). The US 90A/Southwest Rail corridor......

  19. Multi-Laboratory Validation of Estrone (E1) ELISA Methods

    EPA Science Inventory

    This project is a round-robin evaluation of commercially available Enzyme-Linked Immunosorbent Assay (ELISA) technology to quantitatively or qualitatively measure the hormone estrone (E1) in combined animal feeding operation (CAFO) receiving streams. ELISA is meant to be a simpl...

  20. 26 CFR 53.4941(e)-1 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... TAXES (CONTINUED) FOUNDATION AND SIMILAR EXCISE TAXES Taxes on Self-Dealing § 53.4941(e)-1 Definitions... and the liabilities of the parties have been fixed. Thus, for example, if a private foundation gives a disqualified person a binding option on June 15, 1971, to purchase property owned by the foundation at any...

  1. 26 CFR 53.4941(e)-1 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... TAXES (CONTINUED) FOUNDATION AND SIMILAR EXCISE TAXES Taxes on Self-Dealing § 53.4941(e)-1 Definitions... and the liabilities of the parties have been fixed. Thus, for example, if a private foundation gives a disqualified person a binding option on June 15, 1971, to purchase property owned by the foundation at any...

  2. 26 CFR 53.4941(e)-1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TAXES (CONTINUED) FOUNDATION AND SIMILAR EXCISE TAXES Taxes on Self-Dealing § 53.4941(e)-1 Definitions... and the liabilities of the parties have been fixed. Thus, for example, if a private foundation gives a disqualified person a binding option on June 15, 1971, to purchase property owned by the foundation at any...

  3. 26 CFR 53.4941(e)-1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TAXES (CONTINUED) FOUNDATION AND SIMILAR EXCISE TAXES Taxes on Self-Dealing § 53.4941(e)-1 Definitions... and the liabilities of the parties have been fixed. Thus, for example, if a private foundation gives a disqualified person a binding option on June 15, 1971, to purchase property owned by the foundation at any...

  4. 26 CFR 53.4941(e)-1 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... TAXES (CONTINUED) FOUNDATION AND SIMILAR EXCISE TAXES Taxes on Self-Dealing § 53.4941(e)-1 Definitions... and the liabilities of the parties have been fixed. Thus, for example, if a private foundation gives a disqualified person a binding option on June 15, 1971, to purchase property owned by the foundation at any...

  5. 24. SPILLWAY CHANNEL WALLS REINFORCEMENT DETAILS; MONOLITHS E1 TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. SPILLWAY CHANNEL WALLS - REINFORCEMENT DETAILS; MONOLITHS E-1 TO F-4 INCL. & NO. 34. Sheet S-11, June, 1939. File no. SA 342/24(?). - Prado Dam, Spillway, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA

  6. Bursts of energetic electron induced large surface charging observed by Chang'E-1

    NASA Astrophysics Data System (ADS)

    Wang, X. Y.; Zhang, A. B.; Zhang, X. G.; Reme, H.; Kong, L. G.; Zhang, S. Y.; Yu, D. J.; Wang, S. J.; Zhu, G. W.

    2012-10-01

    A relationship between surface charging and bursts of energetic electron (BEE) event is presented in this paper. In a 200 km lunar polar orbit, during quiet time, 0.1-2.0 MeV BEE events were observed by High Energetic Particles Detectors (HPD) on board Chang'E-1, on December 22, 2007, when the spacecraft was within the inner terrestrial magnetosheath. At the same time, a large surface charging of ∼-5.4 kV was observed by Chang'E-1, which was evidenced by increasing the ions energy observed by Solar Wind Ion Detectors (SWIDs). We found that the surface charging is strongly correlated with BEE events, and the potentials of spacecraft surface charging was experientially expressed as U≈3.6×10-5·fT (kV). The BEE events did occur in the solar wind, geomagnetic tail and magnetosheath alternately, whereas the surface charging during the BEE events is in the magnetosheath or transition region of boundaries. Though the observed surface charging was fewer than the BEE events, it is expected that the occurrence of the charging events caused by the bursts of energetic electrons should be more frequent than the Chang'E-1 observations. Meanwhile, the spacecraft charging indicates the lunar surface can be charged to negative kilovolt-scale by the BEE events even in quiet times.

  7. PPARgamma ligands suppress the feedback loop between E2F2 and cyclin-E1.

    PubMed

    Komatsu, Yoko; Ito, Ichiaki; Wayama, Mitsutoshi; Fujimura, Akiko; Akaogi, Kensuke; Machida, Hikaru; Nakajima, Yuka; Kuroda, Takao; Ohmori, Kazuji; Murayama, Akiko; Kimura, Keiji; Yanagisawa, Junn

    2008-05-23

    PPARgamma is a nuclear hormone receptor that plays a key role in the induction of peroxisome proliferation. A number of studies showed that PPARgamma ligands suppress cell cycle progression; however, the mechanism remains to be determined. Here, we showed that PPARgamma ligand troglitazone inhibited G1/S transition in colon cancer cells, LS174T. Troglitazone did not affect on either expression of CDK inhibitor (p18) or Wnt signaling pathway, indicating that these pathways were not involved in the troglitazone-dependent cell cycle arrest. GeneChip and RT-PCR analyses revealed that troglitazone decreased mRNA levels of cell cycle regulatory factors E2F2 and cyclin-E1 whose expression is activated by E2F2. Down-regulation of E2F2 by troglitazone results in decrease of cyclin-E1 transcription, which could inhibit phosphorylation of Rb protein, and consequently evoke the suppression of E2F2 transcriptional activity. Thus, we propose that troglitazone suppresses the feedback loop containing E2F2, cyclin-E1, and Rb protein. PMID:18355447

  8. 76 FR 23644 - Solicitation of Nominations for Members of the Transit Rail Advisory Committee for Safety (TRACS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-27

    ..., FTA issued an initial notice (75 FR 5172) soliciting nominations to serve on TRACS. From that... knowledge base with professionals who have done academic research in the safety field. DATES: Applications... agencies. The FTA Administrator is now seeking to increase the representation from members of the...

  9. 77 FR 75521 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-21

    ... 1, 2005. 70 FR 44256 (Aug. 2, 2005). As explained in the SUPPLEMENTARY INFORMATION section of these... Home Mortgage Disclosure Act (HMDA). 70 FR 12148 (Mar. 11, 2005). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1... the other federal banking agencies in its CRA rule set forth at 12 CFR part 563e. 72 FR 13429 (Mar....

  10. 78 FR 74119 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ... of the Secretary Department of Defense Task Force on the Care, Management, and Transition of... of the Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured Members of the Armed Forces (subsequently referred to as the Task Force)....

  11. 75 FR 61553 - National Transit Database: Amendments to the Urbanized Area Annual Reporting Manual and to the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... Transit (RB), Commuter Bus (CB), Streetcar Rail (SR), and Hybrid Rail (YR). These definitions, like all... overhead catenaries and with frequent stops. Hybrid Rail (YR): This mode is for rail transit systems... Otherwise Classified'' (e.g. slip and fall/electric shock/other) on the S&S-50 Form and on the ``Number...

  12. 77 FR 14364 - Comment Sought on Funding Pilot Program Participants Transitioning Out of the Rural Health Care...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-09

    ... COMMISSION Comment Sought on Funding Pilot Program Participants Transitioning Out of the Rural Health Care... to fund Rural Health Care Pilot Program (Pilot Program) participants who will exhaust funding... year to provide time to establish a process to transition them into the permanent Rural Health...

  13. 77 FR 4787 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-31

    ... Forces. The meeting notice published in the January 24, 2012 edition of the Federal Register (77 FR 3454.... Bannick 9:30-9:45 a.m. Break 9:45-10:45 a.m. OSD Office of Military Community and Family Policy (MCFP) 10... Review & Administration 9:30-9:45 a.m. Break 9:45-10:45 a.m. Continue Site Visit Review...

  14. E1 strength in Pb208 within the shell model

    NASA Astrophysics Data System (ADS)

    Schwengner, R.; Massarczyk, R.; Brown, B. A.; Beyer, R.; Dönau, F.; Erhard, M.; Grosse, E.; Junghans, A. R.; Kosev, K.; Nair, C.; Rusev, G.; Schilling, K. D.; Wagner, A.

    2010-05-01

    The dipole response of the doubly magic nuclide Pb208 was studied in photon-scattering experiments at the electron linear accelerator ELBE with bremsstrahlung produced at kinetic electron energies of 9.0 and 15.0 MeV. The present (γ,γ') data combined with (γ,n) data from the literature are compared with results of shell-model calculations and calculations using a quasiparticle random-phase approximation. The shell-model calculations including (2p-2h) excitations describe the experimental E1 strength well and reproduce the spreading of the giant dipole resonance by applying a small smearing width only.

  15. RP-1 delivered to E-1 Test Stand

    NASA Technical Reports Server (NTRS)

    2010-01-01

    NASA John C. Stennis Space Center employee Dustan Ladner (left) assists tanker driver David Velasco in transferring RP-1 fuel to a 20,000-gallon underground tank at the E-1 Test Stand during a March 30 delivery. The rocket propellant will be used for testing Aerojet AJ26 rocket engines beginning this summer. Stennis is testing the engines for Orbital Sciences Corporation, which has partnered with NASA to provide eight supply missions to the International Space Station through 2015. The partnership is part of NASA's Commercial Orbital Transportation Services initiative to work closer with companies to provide commercial space transport once the space shuttle is retired later this year.

  16. 75 FR 59645 - Radio Broadcast Services and Multichannel Video and Cable Television Service; Clarification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-28

    ... longer pending OMB approval for the sections listed: 73.6027--69 FR 69331, November 29, 2004. 76.5(ll)--61 FR 6137, February 16, 1996. 76.913(b)(1)--62 FR 6495, February 12, 1997. 76.924(e)(1)(iii) and (e)(2)(iii)--61 FR 9367, March 8, 1996. 76.925--60 FR 52119, October 5, 1995. 76.942(f)--60 FR...

  17. Influence of GSTs, CYP2E1 and mEH polymorphisms on 1, 3-butadiene-induced micronucleus frequency in Chinese workers

    SciTech Connect

    Tan Hongshan; Wang Qi; Wang Aihong; Ye Yunjie; Feng Nannan; Feng Xiaoqing; Lu Lingeng; Au, William; Zheng Yuxin; Xia Zhaolin

    2010-09-15

    1,3-butadiene (BD) has been classified as a human carcinogen, however, the relationship between chromosomal damage and its metabolic polymorphisms is not clear. The present study used the CBMN assay to detect chromosomal damage in the peripheral lymphocytes of 166 exposed workers and 41 non-exposed healthy individuals. PCR and PCR-RFLP were applied to detect GSTT1, GSTM1, CYP2E1 c1c2 and mEH Tyr113His, His139Arg polymorphisms. The results demonstrated that the micronucleus (MN) frequency of the exposed workers was significantly higher than controls (P < 0.01). Among the exposed workers, the individuals with high BD exposures are more susceptible to chromosomal damage than those with low exposures (FR = 1.30, 95% CI 1.14-1.53; P < 0.05). Gender-difference was also found in our study: males got lower micronucleus frequency than females. Workers who carried the genotypes of GSTM1 (+), CYP2E1 (c1c2/c2c2) and mEH intermediate (I) group had significantly higher MN frequency than those carrying the genotypes of GSTM1 (-) (FR = 1.29, 95% CI 1.05-1.59; P < 0.05), CYP2E1 (c1c1) (FR = 1.55, 95% CI 1.24-1.93; P < 0.01) or mEH high (H) group (FR = 1.57, 95% CI 1.08-2.34; P < 0.05), respectively. Our data indicated that the current BD exposure level could cause significantly higher MN frequency in workers than controls. Polymorphisms of GSTM1, CYP2E1 and mEH are susceptible to altered chromosome damage.

  18. 78 FR 53187 - Early Scoping Notification for the Alternatives Analysis of the GA 400 Transit Initiative in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-28

    ... that offers an alternative to automobile travel. This notice invites the public and agency officials to... study area by providing a more robust transit network that offers an alternative to automobile...

  19. Discovery of low-lying E1 and M1 strengths in {sup 232}Th

    SciTech Connect

    Adekola, A. S.; Hammond, S. L.; Hill, A.; Karwowski, H. J.; Angell, C. T.; Howell, C. R.; Kwan, E.; Kelley, J. H.

    2011-03-15

    Properties of low-energy dipole states in {sup 232}Th have been investigated with the nuclear resonance fluorescence technique. The present work used monoenergetic {gamma}-ray beams at energies of 2-4 MeV from the high-intensity {gamma}-ray source at Triangle Universities Nuclear Laboratory. Over 40 transitions corresponding to deexcitation to the ground state and first excited state were observed for the first time. Excitation energies, integrated cross sections, decay widths, branching ratios, and transition strengths for those states in {sup 232}Th were determined and compared with quasiparticle random-phase-approximation calculations. A large number of E1 transitions were observed for the first time in actinide nuclei with summed strength of 3.28(69)x10{sup -3} e{sup 2} fm{sup 2}. The observed summed M1 strength of 4.26(63){mu}{sub N}{sup 2} is in good agreement with the other actinides and with the systematics of the scissors mode in deformed rare-earth nuclei.

  20. Crossover Analysis of CHANG'E-1 Laser Altimeter Data

    NASA Astrophysics Data System (ADS)

    Hu, W.; Yue, Z.; Di, K.

    2011-08-01

    This paper presents a preliminary result of crossover analysis and adjustment of Chang'E-1(CE-1) Laser Altimeter (LAM) data of the Moon for global and regional mapping applications. During the operation of Chang'E-1 from November 28, 2007 to December 4, 2008, the laser altimeter acquired 1400 orbital profiles with about 9.12 million altimetric points. In our experiment, we derived more than 1.38 million crossovers from 1395 ground tracks covering the entire lunar surface after eliminating outliers of orbits and altimetric points. A method of least-squares crossover adjustment with a series of basis functions of time (trigonometric functions and polynomials) is developed to reconcile the LAM data by minimizing the crossover residuals globally. The normal equations are very large but sparse; therefore they are stored and solved using sparse matrix technique. In a test area (0°N~60°N, 50°W~0°W), the crossover residuals are reduced from 62.1m to 32.8m, and the quality of the DEM generated from the adjusted LAM data is improved accordingly. We will optimize the method for the global adjustment to generate a high precision consistent global DEM, which can be used as absolute control for lunar mapping with orbital images.

  1. 78 FR 7415 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-01

    ....-10:15 a.m. Break 10:15 a.m.-12:00 p.m. Army Warrior Transition Command Survey Program 12:00 p.m.-1:00 p.m. Additional Questions for Army Warrior Transition Command 1:00 p.m.-2:00 p.m. Break for Lunch 2....defense.gov/rwtf/to view the Charter. Individuals making presentations will be notified by...

  2. Buserelin acetate microparticle dispersion effects drug release and plasma E(1) levels.

    PubMed

    Usami, Makiko; Misawa, Kazumasa; Yagi, Naomi; Sekikawa, Hitoshi; Nabeshima, Toshitaka

    2007-07-18

    We investigated the effect of different dispersion methods on release behavior and efficacy onset following microparticle administration of buserelin acetate (BA) sustained-release injection. In this in vitro release study, the initial dispersion of BA increased with increased stirring speed (p<0.01). Stability of BA was studied over 7 days after BA release. The initial BA release rate was higher (p<0.01) after a 1-min vibration dispersion method (VDM) using a test tube mixer (2000 rpm) compared with the standard dispersion method (SDM) by hand. Without shaking, powder aggregation was observed, and BA release was lower than in either the SDM or VDM methods. In this study using 4-week-old Sprague-Dawley female rats, the initial plasma estrone (E(1)) concentrations were lower (p<0.05) in the VDM method than in the SDM method. Observations by optical microscope and scanning microscope showed no change in microparticle shape or distribution of size induced by SDM, VDM or the ultrasonication dispersion method. These results suggest that different dispersion methods do not change the shape and distribution of microparticle size, but clearly change the BA release rate and the transition in plasma E(1) concentrations that can affect drug efficacy. PMID:17398044

  3. Metabolism of prostaglandin E1 in dog kidneys

    PubMed Central

    Nakano, J.

    1970-01-01

    1. The biotransformation of prostaglandin E1 (PGE1) was studied in the isolated, perfused dog kidneys. 2. An average 43% of PGE1 was converted into the less polar metabolite I by a single passage through the kidney. As the re-circulation of the perfusate continued, PGE1 was converted not only into metabolite I but also the least polar metabolite II. The velocity of the conversion of PGE1 into metabolite I was significantly greater than that into metabolite II. Usually, six passages elapsed before maximum degradation of PGE1 occurred. 3. Further separation with silicic acid column chromatography and gas-liquid chromatography showed that metabolite II consists of two individual metabolites, metabolite IIa and metabolite IIb. 4. The present study indicates that the kidney biotransforms PGE1 rather rapidly into three metabolites which are less polar than PGE1. PMID:5492900

  4. 78 FR 66902 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-07

    ...The Department of Defense is publishing this notice to announce the following Federal Advisory Committee meeting of the Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured Members of the Armed Forces (subsequently referred to as the Task...

  5. 77 FR 63669 - Notice of FTA Transit Program Changes, Authorized Funding Levels and Implementation of the Moving...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ...This notice announces changes in the Federal Transit Administration (FTA) programs in accordance with the Moving Ahead for Progress in the 21st Century Act (MAP-21), which authorizes surface transportation programs of the Department of Transportation for Federal fiscal years (FY) 2013 and 2014. This notice provides preliminary implementation instructions and guidance for the new and revised......

  6. 76 FR 56743 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-14

    ... Team Briefing on TBI management in the Theater. 3 p.m. Department of Labor. 4 p.m. Open. 5 p.m. Closing... of the Secretary Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured Members of the Armed Forces; Meeting AGENCY: Department of...

  7. 78 FR 42156 - Sonoma-Marin Area Rail Transit District-Acquisition Exemption-In Marin County, Cal.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-15

    ... Surface Transportation Board Sonoma-Marin Area Rail Transit District--Acquisition Exemption-- In Marin County, Cal. AGENCY: Surface Transportation Board, DOT. ACTION: Notice of Exemption. SUMMARY: The Board... to Docket No. FD 35732, must be filed with the Surface Transportation Board, 395 E Street...

  8. 77 FR 21098 - Notice of Meeting of the Environmental Financial Advisory Board (EFAB), and Transit-Oriented...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-09

    ... Development Workshop AGENCY: Environmental Protection Agency (EPA). ACTION: Notice. SUMMARY: The United States... 22-23, 2012 and a Transit-Oriented Development Workshop on May 24, 2012. EFAB is an EPA advisory..., Agency priorities and to discuss progress with work projects under EFAB's current Strategic Action...

  9. 78 FR 28580 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-15

    ... of the Secretary Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured Members of the Armed Forces AGENCY: Office of the Assistant Secretary of... Federal Advisory Committee meeting of the Department of Defense Task Force on the Care, Management,...

  10. 75 FR 76422 - Meeting of the Department of Defense Task Force on the Care, Management, and Transition of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... of the Secretary Meeting of the Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and Injured Members of the Armed Forces (Subsequently Referred to as the Task Force) AGENCY: Department of Defense. ACTION: Notice. SUMMARY: Pursuant to Section 10...

  11. 75 FR 59323 - Early Scoping for the Alternatives Analysis of the North Corridor Transit Project in Metropolitan...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-27

    ... (NEPA) and is part of a planning Alternatives Analysis (AA) required by Title 49 United States Code (U.S...-assisted major capital transit investment. The AA process results in the selection or confirmation of a... public comments on the scope of the AA study, including the transportation problems to be addressed,...

  12. 75 FR 44233 - Notice of Proposed Extension of Project Period and Waiver for the National Secondary Transition...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-28

    ... Transition Technical Assistance Center to receive funding from October 1, 2010 through September 30, 2011. DATES: We must receive your comments on or before August 12, 2010. ADDRESSES: Address all comments about... CONTACT. Background On March 8, 2005, the Department published a notice in the Federal Register (70...

  13. 76 FR 57044 - Announcement of Requirements and Registration for “Ensuring Safe Transitions From Hospital to Home”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-15

    ... to stimulate innovative approaches to care transitions and improving patient safety. Nearly one in... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND... Hospital to Home'' Authority: 15 U.S.C. 3719. AGENCY: Office of the National Coordinator for...

  14. 78 FR 48537 - Small Business Innovation Research and Small Business Technology Transfer Programs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... STTR Policy Directives at 77 FR 46806 and 77 FR 46855 respectively. Section 4(a)(3) of these Policy... 16, 2012 at 77 FR 63410. The Transition Rate benchmark sets the minimum ratio of Phase II to Phase I... (77 FR 46806) and the STTR Policy Directive (77 FR 46855). Pravina Raghavan, Deputy...

  15. 78 FR 23204 - Privacy Act of 1974, System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    ... of users and the purpose of such uses: USAID's routine uses, see 42 FR 47371 (September 20, 1977) and 59 FR 52954 (October 20, 1994), apply to this system of records. As additional routine uses for this...); (d); (e)(1); (e)(4)(G), (H), and (I); and (f) of 5 U.S.C. 552a. See 57 FR 38276, 38280-81 (August...

  16. Fast electric dipole transitions in Ra-Ac nuclei

    SciTech Connect

    Ahmad, I.

    1985-01-01

    Lifetime of levels in /sup 225/Ra, /sup 225/Ac, and /sup 227/Ac have been measured by delayed coincidence techniques and these have been used to determine the E1 gamma-ray transition probabilities. The reduced E1 transition probabilities. The reduced E1 transition probabilities in /sup 225/Ra and /sup 225/Ac are about two orders of magnitude larger than the values in mid-actinide nuclei. On the other hand, the E1 rate in /sup 227/Ac is similar to those measured in heavier actinides. Previous studies suggest the presence of octupole deformation in all the three nuclei. The present investigation indicates that fast E1 transitions occur for nuclei with octupole deformation. However, the studies also show that there is no one-to-one correspondence between E1 rate and octupole deformation. 13 refs., 4 figs.

  17. Experimental and computational studies on zwitterionic (E)-1-((4-phenoxyphenyliminio)methyl)naphthalen-2-olate

    NASA Astrophysics Data System (ADS)

    Alpaslan, Gökhan; Macit, Mustafa; Erdönmez, Ahmet; Büyükgüngör, Orhan

    2011-06-01

    The Schiff base compound (E)-1-((4-phenoxyphenyliminio)methyl)naphthalen-2-olate has been synthesized and characterized by IR, UV-Vis, and X-ray single-crystal determination. Molecular geometry of the title compound in the ground state have been calculated using the density functional method (DFT) with 6-31G(d,p) basis set and compared with the experimental data. The calculated results show that the optimized geometry can well reproduce the crystal structure. By using TD-DFT method, electronic absorption spectra of the title compound have been predicted and a good agreement with the TD-DFT method and the experimental ones is determined. Molecular orbital coefficient analyses reveal that the electronic transitions are mainly assigned to n → p∗ and p → p∗ electronic transitions. To investigate the tautomeric stability, optimization calculations at B3LYP/6-31G(d,p) level were performed for the NH and OH forms of the title compound. Calculated results reveal that the OH form is more stable than NH form. In addition, molecular electrostatic potential and non-linear optical properties of the title compound were performed at B3LYP/6-31G(d,p) level of theory.

  18. Human adenovirus 2 E1B-19K and E1B-53K tumor antigens: antipeptide antibodies targeted to the NH2 and COOH termini.

    PubMed Central

    Green, M; Brackmann, K H; Lucher, L A; Symington, J S; Kramer, T A

    1983-01-01

    The human adenovirus 2 (Ad2) transforming region is located in the left 11.1% of the viral genome and encodes two early transcription units, E1A and E1B. Based on the amino acid sequence deduced from the Ad2 E1B DNA sequence (Gingeras et al., J. Biol. Chem. 257:13475-13491, 1982), we have prepared antibodies against synthetic peptides, 8 to 16 amino acids in length, encoded at the NH2 and COOH termini of the major E1B-19K and E1B-53K tumor antigens. The antipeptide antibodies immunoprecipitated the targeted E1B-19K or E1B-53K tumor antigens from extracts of Ad2-infected cells. The specificity of the peptide competition studies. Antipeptide antibodies directed to the NH2 and COOH termini immunoprecipitated the E1B-19K and E1B-53K tumor antigens from two Ad2-transformed rat cell lines, F17 and F4, providing evidence that identical tumor antigens are synthesized in Ad2-infected and Ad2-transformed cells. These results show that the E1B-19K and E1B-53K T antigens are not processed proteolytically at either the NH2 or COOH terminus. Our data provide strong evidence at the protein level that the E1B-19K and E1B-53K tumor antigens partially overlap in DNA sequence, with the E1B-19K initiating translation at the first ATG at nucleotide 1711 in translation reading frame 1 and the E1B-53K tumor antigen initiating translation at the second ATG at nucleotide 2016 in reading frame 3. This confirms the results of others on the N-terminal amino acid sequence of E1B-19K and theoretical deductions based on the DNA sequence. Our findings prove that the large E1B-53K T antigen initiates translation at the second ATG at nucleotide 2016 and not at equally plausible initiation codons located farther downstream at nucleotides 2202 and 2235. Thus, the E1B-53K T antigen is another example of a protein which initiates translation at an internal ATG rather than at the 5'-proximal ATG. Images PMID:6632083

  19. Two classes of human papillomavirus type 16 E1 mutants suggest pleiotropic conformational constraints affecting E1 multimerization, E2 interaction, and interaction with cellular proteins.

    PubMed Central

    Yasugi, T; Vidal, M; Sakai, H; Howley, P M; Benson, J D

    1997-01-01

    Random mutagenesis of human papillomavirus type 16 (HPV16) E1 was used to generate E1 missense mutants defective for interaction with either hUBC9 or 16E1-BP, two cDNAs encoding proteins that have been identified by their ability to interact with HPV16 E1 in two-hybrid assays. hUBC9, the human counterpart of Saccharomyces cerevisiae UBC9, is a ubiquitin-conjugating enzyme known to be involved in cell cycle progression. 16E1-BP encodes a protein of no known function but does contain an ATPase signature motif. Eight hUBC9 or 16E1-BP interaction-defective HPV16 E1 missense mutants were identified and characterized for origin-dependent transient DNA replication, ATPase activity, and various protein-protein interaction phenotypes. Six of these mutant E1 proteins were significantly impaired for replication. Among these, two classes of replication-defective HPV16 E1 missense mutants were observed. One class, represented by the S330R replication-defective mutant (containing an S-to-R change at position 330), remained competent for all protein-protein interactions tested, with the exception of hUBC9 association. Furthermore, this mutant, unlike the other replication-defective HPV16 E1 missense mutants, had a strong dominant negative replication phenotype in transient-replication assays. The other class, represented by five of the missense mutants, was defective for multiple protein-protein interactions, usually including, but not limited to, the interaction defect for which each mutant was originally selected. In many cases, a single missense mutation in one region of HPV16 E1 had pleiotropic effects, even upon activities thought to be associated with other domains of HPV16 E1. This suggests that E1 proteins are not modular but may instead be composed of multiple structurally and/or functionally interdependent domains. PMID:9223484

  20. Lack of cytochrome P450 2E1 (CYP2E1) induction in the rat liver by starvation without coprophagy.

    PubMed

    Chung, H C; Sung, S H; Kim, J S; Kim, Y C; Kim, S G

    2001-03-01

    Starvation potentiates the hepatotoxicity of a variety of small molecules, including chlorinated hydrocarbons and nitrosamines, through the induction of CYP2E1. A change in CYP2E1 expression during starvation may also alter the pharmacokinetic profiles of xenobiotics. Northern blot and Western blot analyses revealed that hepatic CYP2E1 was not induced during starvation in rats placed in metabolic or wire-bottom cages in contrast to the induction of CYP2E1 in animals housed in solid-bottom cages. We studied the effect of coprophagy on the expression of hepatic CYP2E1 during starvation. The extent of coprophagy was 24% in fed rats. Fecal matter of starving rats was reduced to 14% of control and starving rats re-ingested ~1.6 g of feces per day. The effect of fecal matter on CYP2E1 expression (i.e., 1.6 g/kg/day for 3 days) was assessed in fed or starving rats. Starving rats gavaged with fecal matter for 3 days resulted in a 3.5-fold increase in the level of CYP2E1 mRNA, while fed rats gavaged with feces failed to show an increase in the mRNA. The increase in the CYP2E1 mRNA level accompanied the induction of CYP2E1. Starving rats gavaged with methanol extract of feces (500 mg/kg/day for 3 days) showed a 3.3-fold increase in CYP2E1 mRNA level in the liver. These results provide evidence that CYP2E1 is not induced by starvation without coprophagy, raising the contention that the mechanistic basis for CYP2E1 induction by starvation should be reevaluated. PMID:11181486

  1. 77 FR 23667 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-20

    ... Medical Center--Portsmouth, and Iowa Joint Forces Headquarters. 9:30-9:45 a.m. Break. 9:45-10:45 a.m... Briefing. 1:45-2 p.m. Break. 2-3 p.m. 2011 Recommendation Review. Review the 2011 Recommendations see what.... Break. 3:15-5 p.m. Task Force Recommendation Development Review of Transition Outcomes,...

  2. 78 FR 14272 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-05

    ...Under the provisions of the Federal Advisory Committee Act of 1972 (5 U.S.C., Appendix, as amended), the Government in the Sunshine Act of 1976 (5 U.S.C. 552b, as amended), and 41 CFR 102-3.150, the Department of Defense announces that the following Federal Advisory Committee meeting of the Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded, Ill, and......

  3. Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus.

    PubMed

    Enemark, E J; Chen, G; Vaughn, D E; Stenlund, A; Joshua-Tor, L

    2000-07-01

    Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure of the E1 DNA binding domain refined to 1.9 A resolution. Residues critical for DNA binding are located on an extended loop and an alpha helix. We identify the E1 dimerization surface by selective mutations at an E1/E1 interface observed in the crystal and propose a model for the (E1)2-DNA complex. These and other observations suggest how the E1 DNA binding domain orchestrates assembly of the hexameric helicase on the ori. PMID:10949036

  4. 76 FR 52046 - Final Policy Statement on the Eligibility of Pedestrian and Bicycle Improvements Under Federal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... of Joint Development Projects under Federal Transit Law at 72 FR 5788. B. Transit Enhancement... Investment Projects, 75 FR 31383 (June 3, 2010). ] F. Access to Public Transportation for Individuals With... Statement on the Eligibility of Pedestrian and Bicycle Improvements under Federal Transit Law, 74 FR...

  5. The dual effect of adenovirus type 5 E1A 13S protein on NF-kappaB activation is antagonized by E1B 19K.

    PubMed Central

    Schmitz, M L; Indorf, A; Limbourg, F P; Städtler, H; Traenckner, E B; Baeuerle, P A

    1996-01-01

    The genomes of human adenoviruses encode several regulatory proteins, including the two differentially spliced gene products E1A and E1B. Here, we show that the 13S but not the 12S splice variant of E1A of adenovirus type 5 can activate the human transcription factor NF-kappaB in a bimodal fashion. One mode is the activation of NF-kappaB containing the p65 subunit from the cytoplasmic NF-kappaB-IkappaB complex. This activation required reactive oxygen intermediates and the phosphorylation of IkappaBalpha at serines 32 and 36, followed by IkappaBalpha degradation and the nuclear uptake of NF-kappaB. In addition, 13S E1A stimulated the transcriptional activity of the C-terminal 80 amino acids of p65 at a core promoter with either a TATA box or an initiator (INR) element. The C-terminal 80 amino acids of p65 were found to associate with E1A in vitro. The activation of NF-kappaB-dependent reporter gene transcription by E1A was potently suppressed upon coexpression of the E1B 19-kDa protein (19K). E1B 19K prevented both the activation of NF-kappaB and the E1A-mediated transcriptional enhancement of p65. These inhibitory effects were not found for the 55-kDa splice variant of the E1B protein. We suggest that the inductive effect of E1A 13S on the host factor NF-kappaB, whose activation is important for the transcription of various adenovirus genes, must be counteracted by the suppressive effect of E1B 19K so that the adenovirus-infected cell can escape the immune-stimulatory and apoptotic effects of NF-kappaB. PMID:8754803

  6. The Human Adenovirus Type 5 E4orf6/E1B55K E3 Ubiquitin Ligase Complex Enhances E1A Functional Activity.

    PubMed

    Dallaire, Frédéric; Schreiner, Sabrina; Blair, G Eric; Dobner, Thomas; Branton, Philip E; Blanchette, Paola

    2016-01-01

    Human adenovirus (Ad) E1A proteins have long been known as the central regulators of virus infection as well as the major source of adenovirus oncogenic potential. Not only do they activate expression of other early viral genes, they make viral replication possible in terminally differentiated cells, at least in part, by binding to the retinoblastoma (Rb) tumor suppressor family of proteins to activate E2F transcription factors and thus viral and cellular DNA synthesis. We demonstrate in an accompanying article (F. Dallaire et al., mSphere 1:00014-15, 2016) that the human adenovirus E3 ubiquitin ligase complex formed by the E4orf6 and E1B55K proteins is able to mimic E1A activation of E2F transactivation factors. Acting alone in the absence of E1A, the Ad5 E4orf6 protein in complex with E1B55K was shown to bind E2F, disrupt E2F/Rb complexes, and induce hyperphosphorylation of Rb, leading to induction of viral and cellular DNA synthesis, as well as stimulation of early and late viral gene expression and production of viral progeny. While these activities were significantly lower than those exhibited by E1A, we report here that this ligase complex appeared to enhance E1A activity in two ways. First, the E4orf6/E1B55K complex was shown to stabilize E1A proteins, leading to higher levels in infected cells. Second, the complex was demonstrated to enhance the activation of E2F by E1A products. These findings indicated a new role of the E4orf6/E1B55K ligase complex in promoting adenovirus replication. IMPORTANCE Following our demonstration that adenovirus E3 ubiquitin ligase formed by the viral E4orf6 and E1B55K proteins is able to mimic the activation of E2F by E1A, we conducted a series of studies to determine if this complex might also promote the ability of E1A to do so. We found that the complex both significantly stabilizes E1A proteins and also enhances their ability to activate E2F. This finding is of significance because it represents an entirely new function for

  7. The Human Adenovirus Type 5 E4orf6/E1B55K E3 Ubiquitin Ligase Complex Enhances E1A Functional Activity

    PubMed Central

    Dallaire, Frédéric; Schreiner, Sabrina; Blair, G. Eric; Dobner, Thomas; Branton, Philip E.

    2015-01-01

    ABSTRACT Human adenovirus (Ad) E1A proteins have long been known as the central regulators of virus infection as well as the major source of adenovirus oncogenic potential. Not only do they activate expression of other early viral genes, they make viral replication possible in terminally differentiated cells, at least in part, by binding to the retinoblastoma (Rb) tumor suppressor family of proteins to activate E2F transcription factors and thus viral and cellular DNA synthesis. We demonstrate in an accompanying article (F. Dallaire et al., mSphere 1:00014-15, 2016) that the human adenovirus E3 ubiquitin ligase complex formed by the E4orf6 and E1B55K proteins is able to mimic E1A activation of E2F transactivation factors. Acting alone in the absence of E1A, the Ad5 E4orf6 protein in complex with E1B55K was shown to bind E2F, disrupt E2F/Rb complexes, and induce hyperphosphorylation of Rb, leading to induction of viral and cellular DNA synthesis, as well as stimulation of early and late viral gene expression and production of viral progeny. While these activities were significantly lower than those exhibited by E1A, we report here that this ligase complex appeared to enhance E1A activity in two ways. First, the E4orf6/E1B55K complex was shown to stabilize E1A proteins, leading to higher levels in infected cells. Second, the complex was demonstrated to enhance the activation of E2F by E1A products. These findings indicated a new role of the E4orf6/E1B55K ligase complex in promoting adenovirus replication. IMPORTANCE Following our demonstration that adenovirus E3 ubiquitin ligase formed by the viral E4orf6 and E1B55K proteins is able to mimic the activation of E2F by E1A, we conducted a series of studies to determine if this complex might also promote the ability of E1A to do so. We found that the complex both significantly stabilizes E1A proteins and also enhances their ability to activate E2F. This finding is of significance because it represents an entirely new

  8. The adenovirus E1A proteins induce apoptosis, which is inhibited by the E1B 19-kDa and Bcl-2 proteins.

    PubMed Central

    Rao, L; Debbas, M; Sabbatini, P; Hockenbery, D; Korsmeyer, S; White, E

    1992-01-01

    Cooperation between the adenovirus E1A and E1B oncogenes is required for transformation of primary quiescent rodent cells. Although expression of E1A alone will stimulate cell proliferation sufficient to initiate transformed focus formation, proliferation fails to be sustained and foci degenerate. Coexpression of either the 19-kDa or 55-kDa E1B oncoproteins with E1A permits high-frequency transformation by overcoming this cytotoxic response. Without E1B 19-kDa protein expression, however, transformants remain susceptible to induction of cell death. Rapid loss of viability is coincident with nucleolytic cleavage of DNA in intranucleosomal regions and chromatin condensation, hallmarks of programmed cell death (apoptosis). Furthermore, overexpression of a known suppressor of apoptosis, the Bcl-2 protooncogene, can rescue E1A-induced focus degeneration. Thus E1A-dependent stimulation of cell proliferation is accompanied by apoptosis and thereby insufficient to singly induce transformation. High-frequency transformation requires a second function encoded by the E1B 19-kDa protein to block apoptosis. Images PMID:1457005

  9. Sequence-independent autoregulation of the adenovirus type 5 E1A transcription unit.

    PubMed Central

    Hearing, P; Shenk, T

    1985-01-01

    The adenovirus E1A gene is known to be autoregulated at the level of transcription. Autoregulation was found to be mediated by products of the E1A 13S mRNA, which induced a fivefold increase in E1A transcription rate. Deletion analysis suggested that the autoregulation did not require any specific sequence in the E1A transcriptional control region. This conclusion was reinforced by the demonstration that a cellular alpha-globin gene substituted for the E1A gene on the adenovirus chromosome was also positively regulated by E1A gene products. Images PMID:2943984

  10. Metabolic inactivation of resolvin E1 and stabilization of its anti-inflammatory actions.

    PubMed

    Arita, Makoto; Oh, Sungwhan F; Chonan, Tomomichi; Hong, Song; Elangovan, Siva; Sun, Yee-Ping; Uddin, Jasim; Petasis, Nicos A; Serhan, Charles N

    2006-08-11

    The resolvins (Rv) are lipid mediators derived from omega-3 polyunsaturated fatty acids that act within a local inflammatory milieu to stop leukocyte recruitment and promote resolution. Resolvin E1 (RvE1; (5S,12R,18R)-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid) is an oxygenase product derived from omega-3 eicosapentaenoic acid that displays potent anti-inflammation/pro-resolution actions in vivo. Here, we determined whether oxidoreductase enzymes catalyze the conversion of RvE1 and assessed the biological activity of the RvE1 metabolite. With NAD+ as a cofactor, recombinant 15-hydroxyprostaglandin dehydrogenase acted as an 18-hydroxyl dehydrogenase to form 18-oxo-RvE1. In the murine lung, dehydrogenation of the hydroxyl group at carbon 18 position to form 18-oxo-RvE1 represented the major initial metabolic route for RvE1. At a concentration where RvE1 potently reduced polymorphonuclear leukocyte (PMN) recruitment in zymosan-induced peritonitis, 18-oxo-RvE1 was devoid of activity. In human neutrophils, carbon 20 hydroxylation of RvE1 was the main route of conversion. An RvE1 analog, i.e. 19-(p-fluorophenoxy)-RvE1, was synthesized that resisted rapid metabolic inactivation and proved to retain biological activity reducing PMN infiltration and pro-inflammatory cytokine/chemokine production in vivo. These results established the structure of a novel RvE1 initial metabolite, indicating that conversion of RvE1 to the oxo product represents a mode of RvE1 inactivation. Moreover, the designed RvE1 analog, which resisted further metabolism/inactivation, could be a useful tool to evaluate the actions of RvE1 in complex disease models. PMID:16757471

  11. Improved Plant-based Production of E1 endoglucanase Using Potato: Expression Optimization and Tissue Targeting

    SciTech Connect

    Dai, Ziyu; Hooker, Brian S.; Anderson, Daniel B.; Thomas, Steven R.

    2000-06-01

    Optimization of Acidothermus cellulolyticus endoglucanase (E1) gene expression in transgenic potato (Solanum tuberosum L.) was examined in this study, where the E1 coding sequence was transcribed under control of a leaf specific promoter (tomato RbcS-3C) or the Mac promoter (a hybrid promoter of mannopine synthase promoter and cauliflower mosaic virus 35S promoter enhancer region). Average E1 activity in leaf extracts of potato transformants, in which E1 protein was targeted by a chloroplast signal peptide and an apoplast signal peptide were much higher than those by an E1 native signal peptide and a vacuole signal peptide. E1 protein accumulated up to 2.6% of total leaf soluble protein, where E1 gene was under control of the RbcS-3C promoter, alfalfa mosaic virus 5-untranslated leader, and RbcS-2A signal peptide. E1 protein production, based on average E1 activity and E1 protein accumulation in leaf extracts, is higher in potato than those measured previously in transgenic tobacco bearing the same transgene constructs. Comparisons of E1 activity, protein accumulation, and relative mRNA levels showed that E1 expression under control of tomato RbcS-3C promoter was specifically localized in leaf tissues, while E1 gene was expressed in both leaf and tuber tissues under control of Mac promoter. This suggests dual-crop applications in which potato vines serve as enzyme production `bioreactors' while tubers are preserved for culinary applications.

  12. Functional conservation and diversification of the soybean maturity gene E1 and its homologs in legumes

    PubMed Central

    Zhang, Xingzheng; Zhai, Hong; Wang, Yaying; Tian, Xiaojie; Zhang, Yupeng; Wu, Hongyan; Lü, Shixiang; Yang, Guang; Li, Yuqiu; Wang, Lu; Hu, Bo; Bu, Qingyun; Xia, Zhengjun

    2016-01-01

    Gene regulatory networks involved in flowering time and photoperiodic responses in legumes remain unknown. Although the major maturity gene E1 has been successfully deciphered in soybean, knowledge on the functional conservation of this gene is limited to a certain extent to E1 homologs in legumes. The ectopic expression of Phvul.009G204600 (PvE1L), an E1 homolog from common bean, delayed the onset of flowering in soybean. By contrast, the ectopic expression of Medtr2g058520 (MtE1L) from Medicago truncatula did not affect the flowering of soybean. Characterization of the late-flowering mte1l mutant indicated that MtE1L promoted flowering in Medicago truncatula. Moreover, all transgenic E1, PvE1L and MtE1L soybean lines exhibited phenotypic changes in terms of plant height. Transgenic E1 or PvE1L plants were taller than the wild-type, whereas transgenic MtE1L plants produced dwarf phenotype with few nodes and short internode. Thus, functional conservation and diversification of E1 family genes from legumes in the regulation of flowering and plant growth may be associated with lineage specification and genomic duplication. PMID:27405888

  13. Atg8 Transfer from Atg7 to Atg3: A Distinctive E1-E2 Architecture and Mechanism in the Autophagy Pathway

    SciTech Connect

    Taherbhoy, Asad M.; Tait, Stephen W.; Kaiser, Stephen E.; Williams, Allison H.; Deng, Alan; Nourse, Amanda; Hammel, Michal; Kurinov, Igor; Rock, Charles O.; Green, Douglas R.; Schulman, Brenda A.

    2012-07-11

    Atg7 is a noncanonical, homodimeric E1 enzyme that interacts with the noncanonical E2 enzyme, Atg3, to mediate conjugation of the ubiquitin-like protein (UBL) Atg8 during autophagy. Here we report that the unique N-terminal domain of Atg7 (Atg7{sup NTD}) recruits a unique 'flexible region' from Atg3 (Atg3{sup FR}). The structure of an Atg7{sup NTD}-Atg3{sup FR} complex reveals hydrophobic residues from Atg3 engaging a conserved groove in Atg7, important for Atg8 conjugation. We also report the structure of the homodimeric Atg7 C-terminal domain, which is homologous to canonical E1s and bacterial antecedents. The structures, SAXS, and crosslinking data allow modeling of a full-length, dimeric (Atg7 {approx} Atg8-Atg3){sub 2} complex. The model and biochemical data provide a rationale for Atg7 dimerization: Atg8 is transferred in trans from the catalytic cysteine of one Atg7 protomer to Atg3 bound to the N-terminal domain of the opposite Atg7 protomer within the homodimer. The studies reveal a distinctive E1 {approx} UBL-E2 architecture for enzymes mediating autophagy.

  14. Cell-based analysis of Chikungunya virus E1 protein in membrane fusion

    PubMed Central

    2012-01-01

    Background Chikungunya fever is a pandemic disease caused by the mosquito-borne Chikungunya virus (CHIKV). E1 glycoprotein mediation of viral membrane fusion during CHIKV infection is a crucial step in the release of viral genome into the host cytoplasm for replication. How the E1 structure determines membrane fusion and whether other CHIKV structural proteins participate in E1 fusion activity remain largely unexplored. Methods A bicistronic baculovirus expression system to produce recombinant baculoviruses for cell-based assay was used. Sf21 insect cells infected by recombinant baculoviruses bearing wild type or single-amino-acid substitution of CHIKV E1 and EGFP (enhanced green fluorescence protein) were employed to investigate the roles of four E1 amino acid residues (G91, V178, A226, and H230) in membrane fusion activity. Results Western blot analysis revealed that the E1 expression level and surface features in wild type and mutant substituted cells were similar. However, cell fusion assay found that those cells infected by CHIKV E1-H230A mutant baculovirus showed little fusion activity, and those bearing CHIKV E1-G91D mutant completely lost the ability to induce cell-cell fusion. Cells infected by recombinant baculoviruses of CHIKV E1-A226V and E1-V178A mutants exhibited the same membrane fusion capability as wild type. Although the E1 expression level of cells bearing monomeric-E1-based constructs (expressing E1 only) was greater than that of cells bearing 26S-based constructs (expressing all structural proteins), the sizes of syncytial cells induced by infection of baculoviruses containing 26S-based constructs were larger than those from infections having monomeric-E1 constructs, suggesting that other viral structure proteins participate or regulate E1 fusion activity. Furthermore, membrane fusion in cells infected by baculovirus bearing the A226V mutation constructs exhibited increased cholesterol-dependences and lower pH thresholds. Cells bearing the V178

  15. Transition Planning

    ERIC Educational Resources Information Center

    Statfeld, Jenna L.

    2011-01-01

    Post-school transition is the movement of a child with disabilities from school to activities that occur after the completion of school. This paper provides information about: (1) post-school transition; (2) transition plan; (3) transition services; (4) transition planning; (5) vocational rehabilitation services; (6) services that are available…

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    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... sale price. 48.4216(e)-1 Section 48.4216(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF... Provisions Applicable to Manufacturers Taxes § 48.4216(e)-1 Exclusion of local advertising charges from sale price. (a) In general. Section 4216(e) deals with the treatment to be accorded charges made by...

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    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... sale price. 48.4216(e)-1 Section 48.4216(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF... Provisions Applicable to Manufacturers Taxes § 48.4216(e)-1 Exclusion of local advertising charges from sale price. (a) In general. Section 4216(e) deals with the treatment to be accorded charges made by...

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    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... by the Interstate Commerce Commission (49 CFR Part 1201), but only if (i) such unit exclusively moves... rolling stock. 1.263(e)-1 Section 1.263(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Items Not Deductible § 1.263(e)-1 Expenditures...

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    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 11 2011-04-01 2011-04-01 false Non-pro rata redemptions. 1.1059(e)-1 Section 1... (CONTINUED) INCOME TAXES (CONTINUED) Special Rules § 1.1059(e)-1 Non-pro rata redemptions. (a) In general... under section 1059(e)(1). For example, if a redemption of stock is not pro rata as to all...

  20. 26 CFR 1.669(e)-1A - Pro rata portion of taxes deemed distributed.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Pro rata portion of taxes deemed distributed. 1.669(e)-1A Section 1.669(e)-1A Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY... Applicable to Taxable Years Beginning Before January 1, 1969 § 1.669(e)-1A Pro rata portion of taxes...

  1. 26 CFR 1.509(e)-1 - Definition of gross investment income.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Definition of gross investment income. 1.509(e)-1 Section 1.509(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Private Foundations § 1.509(e)-1 Definition of...

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    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 7 2011-04-01 2009-04-01 true Definition of gross investment income. 1.509(e)-1 Section 1.509(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Private Foundations § 1.509(e)-1 Definition of...

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    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Information reporting requirements for tax-exempt bonds. 1.149(e)-1 Section 1.149(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Tax Exemption Requirements for State and Local Bonds § 1.149(e)-1...

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    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... 1.6050E-1 Section 1.6050E-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Information Returns § 1.6050E-1 Reporting of... individual in any calendar year. (b) Definitions. For purposes of this section— (1) The term refund...

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    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... 1.6050E-1 Section 1.6050E-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Information Returns § 1.6050E-1 Reporting of... individual in any calendar year. (b) Definitions. For purposes of this section— (1) The term refund...

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    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... 1.6050E-1 Section 1.6050E-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Information Returns § 1.6050E-1 Reporting of State and local... year. (b) Definitions. For purposes of this section— (1) The term refund officer means the officer...

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    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... bona fide resignation of directors. 270.10e-1 Section 270.10e-1 Commodity and Securities Exchanges....10e-1 Death, disqualification, or bona fide resignation of directors. If a registered investment company, by reason of the death, disqualification, or bona fide resignation of any director, does not...

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    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... state they were in on completion of the exempt function (e.g., the sale of silver waste produced in the...(e)-1 Section 1.501(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(e)-1...

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    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... state they were in on completion of the exempt function (e.g., the sale of silver waste produced in the...(e)-1 Section 1.501(e)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(e)-1...

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    Code of Federal Regulations, 2013 CFR

    2013-04-01

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