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Sample records for frequency harmonic imaging

  1. 20 MHz/40 MHz Dual Element Transducers for High Frequency Harmonic Imaging

    PubMed Central

    Kim, Hyung Ham; Cannata, Jonathan M.; Liu, Ruibin; Chang, Jin Ho; Silverman, Ronald H.; Shung, K. Kirk

    2009-01-01

    Concentric annular type dual element transducers for second harmonic imaging at 20 MHz / 40 MHz were designed and fabricated to improve spatial resolution and depth of penetration for ophthalmic imaging applications. The outer ring element was designed to transmit the 20 MHz signal and the inner circular element was designed to receive the 40 MHz second harmonic signal. Lithium niobate (LiNbO3), with its low dielectric constant, was used as the piezoelectric material to achieve good electrical impedance matching. Double matching layers and conductive backing were used and optimized by KLM modeling to achieve high sensitivity and wide bandwidth for harmonic imaging and superior time-domain characteristics. Prototype transducers were fabricated and evaluated quantitatively and clinically. The average measured center frequency for the transmit ring element was 21 MHz and the one-way –3 dB bandwidth was greater than 50%. The 40 MHz receive element functioned at 31 MHz center frequency with acceptable bandwidth to receive attenuated and frequency downshifted harmonic signal. The lateral beam profile for the 20 MHz ring elements at the focus matched the Field II simulated results well, and the effect of outer ring diameter was also examined. Images of a posterior segment of an excised pig eye and a choroidal nevus of human eye were obtained both for single element and dual element transducers and compared to demonstrate the advantages of dual element harmonic imaging. PMID:19126492

  2. Design factors of intravascular dual frequency transducers for super-harmonic contrast imaging and acoustic angiography

    NASA Astrophysics Data System (ADS)

    Ma, Jianguo; Martin, K. Heath; Li, Yang; Dayton, Paul A.; Shung, K. Kirk; Zhou, Qifa; Jiang, Xiaoning

    2015-05-01

    Imaging of coronary vasa vasorum may lead to assessment of the vulnerable plaque development in diagnosis of atherosclerosis diseases. Dual frequency transducers capable of detection of microbubble super-harmonics have shown promise as a new contrast-enhanced intravascular ultrasound (CE-IVUS) platform with the capability of vasa vasorum imaging. Contrast-to-tissue ratio (CTR) in CE-IVUS imaging can be closely associated with low frequency transmitter performance. In this paper, transducer designs encompassing different transducer layouts, transmitting frequencies, and transducer materials are compared for optimization of imaging performance. In the layout selection, the stacked configuration showed superior super-harmonic imaging compared with the interleaved configuration. In the transmitter frequency selection, a decrease in frequency from 6.5 MHz to 5 MHz resulted in an increase of CTR from 15 dB to 22 dB when receiving frequency was kept constant at 30 MHz. In the material selection, the dual frequency transducer with the lead magnesium niobate-lead titanate (PMN-PT) 1-3 composite transmitter yielded higher axial resolution compared to single crystal transmitters (70 μm compared to 150 μm pulse length). These comparisons provide guidelines for the design of intravascular acoustic angiography transducers.

  3. Design factors of intravascular dual frequency transducers for super-harmonic contrast imaging and acoustic angiography.

    PubMed

    Ma, Jianguo; Martin, K Heath; Li, Yang; Dayton, Paul A; Shung, K Kirk; Zhou, Qifa; Jiang, Xiaoning

    2015-05-01

    Imaging of coronary vasa vasorum may lead to assessment of the vulnerable plaque development in diagnosis of atherosclerosis diseases. Dual frequency transducers capable of detection of microbubble super-harmonics have shown promise as a new contrast-enhanced intravascular ultrasound (CE-IVUS) platform with the capability of vasa vasorum imaging. Contrast-to-tissue ratio (CTR) in CE-IVUS imaging can be closely associated with low frequency transmitter performance. In this paper, transducer designs encompassing different transducer layouts, transmitting frequencies, and transducer materials are compared for optimization of imaging performance. In the layout selection, the stacked configuration showed superior super-harmonic imaging compared with the interleaved configuration. In the transmitter frequency selection, a decrease in frequency from 6.5 MHz to 5 MHz resulted in an increase of CTR from 15 dB to 22 dB when receiving frequency was kept constant at 30 MHz. In the material selection, the dual frequency transducer with the lead magnesium niobate-lead titanate (PMN-PT) 1-3 composite transmitter yielded higher axial resolution compared to single crystal transmitters (70 μm compared to 150 μm pulse length). These comparisons provide guidelines for the design of intravascular acoustic angiography transducers. PMID:25856384

  4. Design factors of intravascular dual frequency transducers for super-harmonic contrast imaging and acoustic angiography

    PubMed Central

    Ma, Jianguo; Martin, K. Heath; Li, Yang; Dayton, Paul A.; Shung, K. Kirk; Zhou, Qifa; Jiang, Xiaoning

    2015-01-01

    Imaging of coronary vasa vasorum may lead to assessment of the vulnerable plaque development in diagnosis of atherosclerosis diseases. Dual frequency transducers capable of detection of microbubble super-harmonics have shown promise as a new contrast-enhanced intravascular ultrasound (CE-IVUS) platform with the capability of vasa vasorum imaging. Contrast-to-tissue ratio (CTR) in CE-IVUS imaging can be closely associated with the low frequency transmitter performance. In this paper, transducer designs encompassing different transducer layouts, transmitting frequencies, and transducer materials are compared for optimization of imaging performance. In the layout selection, the stacked configuration showed superior super-harmonic imaging compared with the interleaved configuration. In the transmitter frequency selection, a decrease in frequency from 6.5 MHz to 5 MHz resulted in an increase of CTR from 15 dB to 22 dB when receiving frequency was kept constant at 30 MHz. In the material selection, the dual frequency transducer with the lead magnesium niobate-lead titanate (PMN-PT) 1-3 composite transmitter yielded higher axial resolution compared to single crystal transmitters (70 μm compared to 150 μm pulse length). These comparisons provide guidelines for design of intravascular acoustic angiography transducers. PMID:25856384

  5. Ultrasound harmonic imaging with reducing speckle noise by spatial-frequency compounding approach

    NASA Astrophysics Data System (ADS)

    Guo, Wei; Wang, Yuanyuan; Yu, Jinhua

    2015-12-01

    Speckle noise is a phenomenon inherent in any coherent imaging process and decreases the signal-to-noise ratio (SNR), which brings down the imaging quality. Speckle noise reduction is particularly important in the tissue harmonic imaging (THI) since it has the lower energy and the poorer SNR than the fundamental imaging (FI). Recently plane wave imaging (PWI) has been widely explored. Since the entire imaging region can be covered in one emission, the frame rate increases greatly. In PWI, speckle can be reduced by incoherently averaging images with different speckle patterns. Such images can be acquired by varying the angle from which a target is imaged (spatial compounding, SC) or by changing the spectrum of the pulse (frequency compounding, FC). In this paper we demonstrate here that each approach is only a partial solution and that combining them provides a better result than applying either approach separately. We propose a spatial-frequency compounding (SFC) method for THI. The new method brings a good speckle suppression result. To illustrate the performance of our method, experiments have been conducted on the simulated data. A nonlinear simulation platform based on the full-wave model is used in the harmonic imaging simulation. Results show that our method brings the SNR an improvement of up to 50% in comparison with the single frame HI while maintaining a far better performance in both terms of resolution and contrast than the FI. Similar results can be obtained from our further experiments.

  6. Harmonic Frequency Lowering

    PubMed Central

    Kirchberger, Martin

    2016-01-01

    A novel algorithm for frequency lowering in music was developed and experimentally tested in hearing-impaired listeners. Harmonic frequency lowering (HFL) combines frequency transposition and frequency compression to preserve the harmonic content of music stimuli. Listeners were asked to make judgments regarding detail and sound quality in music stimuli. Stimuli were presented under different signal processing conditions: original, low-pass filtered, HFL, and nonlinear frequency compressed. Results showed that participants reported perceiving the most detail in the HFL condition. In addition, there was no difference in sound quality across conditions. PMID:26834122

  7. A parallel adaptive finite element simplified spherical harmonics approximation solver for frequency domain fluorescence molecular imaging.

    PubMed

    Lu, Yujie; Zhu, Banghe; Shen, Haiou; Rasmussen, John C; Wang, Ge; Sevick-Muraca, Eva M

    2010-08-21

    Fluorescence molecular imaging/tomography may play an important future role in preclinical research and clinical diagnostics. Time- and frequency-domain fluorescence imaging can acquire more measurement information than the continuous wave (CW) counterpart, improving the image quality of fluorescence molecular tomography. Although diffusion approximation (DA) theory has been extensively applied in optical molecular imaging, high-order photon migration models need to be further investigated to match quantitation provided by nuclear imaging. In this paper, a frequency-domain parallel adaptive finite element solver is developed with simplified spherical harmonics (SP(N)) approximations. To fully evaluate the performance of the SP(N) approximations, a fast time-resolved tetrahedron-based Monte Carlo fluorescence simulator suitable for complex heterogeneous geometries is developed using a convolution strategy to realize the simulation of the fluorescence excitation and emission. The validation results show that high-order SP(N) can effectively correct the modeling errors of the diffusion equation, especially when the tissues have high absorption characteristics or when high modulation frequency measurements are used. Furthermore, the parallel adaptive mesh evolution strategy improves the modeling precision and the simulation speed significantly on a realistic digital mouse phantom. This solver is a promising platform for fluorescence molecular tomography using high-order approximations to the radiative transfer equation. PMID:20671350

  8. Dual-frequency super harmonic imaging piezoelectric transducers for transrectal ultrasound

    NASA Astrophysics Data System (ADS)

    Kim, Jinwook; Li, Sibo; Kasoji, Sandeep; Dayton, Paul A.; Jiang, Xiaoning

    2015-03-01

    In this paper, a 2/14 MHz dual-frequency single-element transducer and a 2/22 MHz sub-array (16/48-elements linear array) transducer were developed for contrast enhanced super-harmonic ultrasound imaging of prostate cancer with the low frequency ultrasound transducer as a transmitter for contrast agent (microbubble) excitation and the high frequency transducer as a receiver for detection of nonlinear responses from microbubbles. The 1-3 piezoelectric composite was used as active materials of the single-element transducers due to its low acoustic impedance and high coupling factor. A high dielectric constant PZT ceramic was used for the sub-array transducer due to its high dielectric property induced relatively low electrical impedance. The possible resonance modes of the active elements were estimated using finite element analysis (FEA). The pulse-echo response, peak-negative pressure and bubble response were tested, followed by in vitro contrast imaging tests using a graphite-gelatin tissue-mimicking phantom. The single-element dual frequency transducer (8 × 4 × 2 mm3) showed a -6 dB fractional bandwidth of 56.5% for the transmitter, and 41.8% for the receiver. A 2 MHz-transmitter (730 μm pitch and 6.5 mm elevation aperture) and a 22 MHz-receiver (240 μm pitch and 1.5 mm aperture) of the sub-array transducer exhibited -6 dB fractional bandwidth of 51.0% and 40.2%, respectively. The peak negative pressure at the far field was about -1.3 MPa with 200 Vpp, 1-cycle 2 MHz burst, which is high enough to excite microbubbles for nonlinear responses. The 7th harmonic responses from micro bubbles were successfully detected in the phantom imaging test showing a contrast-to-tissue ratio (CTR) of 16 dB.

  9. Color harmonization for images

    NASA Astrophysics Data System (ADS)

    Tang, Zhen; Miao, Zhenjiang; Wan, Yanli; Wang, Zhifei

    2011-04-01

    Color harmonization is an artistic technique to adjust a set of colors in order to enhance their visual harmony so that they are aesthetically pleasing in terms of human visual perception. We present a new color harmonization method that treats the harmonization as a function optimization. For a given image, we derive a cost function based on the observation that pixels in a small window that have similar unharmonic hues should be harmonized with similar harmonic hues. By minimizing the cost function, we get a harmonized image in which the spatial coherence is preserved. A new matching function is proposed to select the best matching harmonic schemes, and a new component-based preharmonization strategy is proposed to preserve the hue distribution of the harmonized images. Our approach overcomes several shortcomings of the existing color harmonization methods. We test our algorithm with a variety of images to demonstrate the effectiveness of our approach.

  10. In vitro and in vivo tissue harmonic images obtained with parallel transmit beamforming by means of orthogonal frequency division multiplexing.

    PubMed

    Demi, Libertario; Ramalli, Alessandro; Giannini, Gabriele; Mischi, Massimo

    2015-01-01

    In classic pulse-echo ultrasound imaging, the data acquisition rate is limited by the speed of sound. To overcome this, parallel beamforming techniques in transmit (PBT) and in receive (PBR) mode have been proposed. In particular, PBT techniques, based on the transmission of focused beams, are more suitable for harmonic imaging because they are capable of generating stronger harmonics. Recently, orthogonal frequency division multiplexing (OFDM) has been investigated as a means to obtain parallel beamformed tissue harmonic images. To date, only numerical studies and experiments in water have been performed, hence neglecting the effect of frequencydependent absorption. Here we present the first in vitro and in vivo tissue harmonic images obtained with PBT by means of OFDM, and we compare the results with classic B-mode tissue harmonic imaging. The resulting contrast-to-noise ratio, here used as a performance metric, is comparable. A reduction by 2 dB is observed for the case in which three parallel lines are reconstructed. In conclusion, the applicability of this technique to ultrasonography as a means to improve the data acquisition rate is confirmed. PMID:25585405

  11. Second harmonic inversion for ultrasound contrast harmonic imaging

    NASA Astrophysics Data System (ADS)

    Pasovic, Mirza; Danilouchkine, Mike; Faez, Telli; van Neer, Paul L. M. J.; Cachard, Christian; van der Steen, Antonius F. W.; Basset, Olivier; de Jong, Nico

    2011-06-01

    Ultrasound contrast agents (UCAs) are small micro-bubbles that behave nonlinearly when exposed to an ultrasound wave. This nonlinear behavior can be observed through the generated higher harmonics in a back-scattered echo. In past years several techniques have been proposed to detect or image harmonics produced by UCAs. In these proposed works, the harmonics generated in the medium during the propagation of the ultrasound wave played an important role, since these harmonics compete with the harmonics generated by the micro-bubbles. We present a method for the reduction of the second harmonic generated during nonlinear-propagation-dubbed second harmonic inversion (SHI). A general expression for the suppression signals is also derived. The SHI technique uses two pulses, p' and p'', of the same frequency f0 and the same amplitude P0 to cancel out the second harmonic generated by nonlinearities of the medium. Simulations show that the second harmonic is reduced by 40 dB on a large axial range. Experimental SHI B-mode images, from a tissue-mimicking phantom and UCAs, show an improvement in the agent-to-tissue ratio (ATR) of 20 dB compared to standard second harmonic imaging and 13 dB of improvement in harmonic power Doppler.

  12. Impact of harmonics on the interpolated DFT frequency estimator

    NASA Astrophysics Data System (ADS)

    Belega, Daniel; Petri, Dario; Dallet, Dominique

    2016-01-01

    The paper investigates the effect of the interference due to spectral leakage on the frequency estimates returned by the Interpolated Discrete Fourier Transform (IpDFT) method based on the Maximum Sidelobe Decay (MSD) windows when harmonically distorted sine-waves are analyzed. The expressions for the frequency estimation error due to both the image of the fundamental tone and harmonics, and the frequency estimator variance due to the combined effect of both the above disturbances and wideband noise are derived. The achieved expressions allow us to identify which harmonics significantly contribute to frequency estimation uncertainty. A new IpDFT-based procedure capable to compensate all the significant effects of harmonics on the frequency estimation accuracy is then proposed. The derived theoretical results are verified through computer simulations. Moreover, the accuracy of the proposed procedure is compared with those of other state-of-the-art frequency estimation methods by means of both computer simulations and experimental results.

  13. Second harmonic generation and sum frequency generation

    SciTech Connect

    Pellin, M.J.; Biwer, B.M.; Schauer, M.W.; Frye, J.M.; Gruen, D.M.

    1990-01-01

    Second harmonic generation and sum frequency generation are increasingly being used as in situ surface probes. These techniques are coherent and inherently surface sensitive by the nature of the mediums response to intense laser light. Here we will review these two techniques using aqueous corrosion as an example problem. Aqueous corrosion of technologically important materials such as Fe, Ni and Cr proceeds from a reduced metal surface with layer by layer growth of oxide films mitigated by compositional changes in the chemical makeup of the growing film. Passivation of the metal surface is achieved after growth of only a few tens of atomic layers of metal oxide. Surface Second Harmonic Generation and a related nonlinear laser technique, Sum Frequency Generation have demonstrated an ability to probe the surface composition of growing films even in the presence of aqueous solutions. 96 refs., 4 figs.

  14. Imaging with Second-Harmonic Generation Nanoparticles

    NASA Astrophysics Data System (ADS)

    Hsieh, Chia-Lung

    Second-harmonic generation nanoparticles show promise as imaging probes due to their coherent and stable signal with a broad flexibility in the choice of excitation wavelength. In this thesis, we developed and demonstrated barium titanate nanoparticles as second-harmonic radiation imaging probes. We studied the absolute second-harmonic generation efficiency of the nanoparticles on single-particle level. The polarization dependent second-harmonic signal of single nanoparticles was studied in detail. From the measured polar response, we were able to find the orientation of the nanoparticle. We developed a biochemical interface for using the second-harmonic nanoprobes as biomarkers, including in vitro cellular imaging and in vivo live animal imaging. The nanoparticles were surface functionalized with primary amine groups for stable colloidal dispersion. We achieved specific labeling of the second-harmonic nanoprobes via immunostaining where the antibodies were covalently conjugated onto the nanoparticles. We observed no toxicity of the functionalized nanoparticles to biological cells. The coherent second-harmonic signal radiated from the nanoparticles offers opportunities for new imaging techniques. Using interferometric detection, namely harmonic holography, both amplitude and phase of the second-harmonic field can be captured. Through digital beam propagation, three-dimensional field distribution, reflecting three-dimensional distribution of the nanoparticles, can be reconstructed. We achieved a scan-free three-dimensional imaging of nanoparticles in biological cells with sub-micron spatial resolution by using the harmonic holographic microscope. We further exploited the coherent second-harmonic signal for imaging through scattering media by performing optical phase conjugation of the second-harmonic signal. We demonstrated an all-digital optical phase conjugation of the second-harmonic signal originated from a nanoparticle by combining harmonic holography and

  15. Dark-field third-harmonic imaging

    NASA Astrophysics Data System (ADS)

    Doronina-Amitonova, L. V.; Lanin, A. A.; Fedotov, I. V.; Ivashkina, O. I.; Zots, M. A.; Fedotov, A. B.; Anokhin, K. V.; Zheltikov, A. M.

    2013-08-01

    Coherent cancellation of third-harmonic generation (THG) in a tightly focused laser beam is shown to enable a label-free imaging of individual neurons in representative brain tissues. The intrinsic coherence of third-harmonic buildup and cancellation combined with the nonlinear nature of the process enhances the locality of the dark signal in THG, translating into a remarkable sharpness of dark-field THG images. Unique advantages of this technique for high-contrast subcellular-resolution neuroimaging are demonstrated by comparing THG images of hippocampus and somatosensory cortex in a mouse brain with images visualizing fluorescent protein biomarkers.

  16. Perfusion harmonic imaging of the human brain

    NASA Astrophysics Data System (ADS)

    Metzler, Volker H.; Seidel, Guenter; Wiesmann, Martin; Meyer, Karsten; Aach, Til

    2003-05-01

    The fast visualisation of cerebral microcirculation supports diagnosis of acute cerebrovascular diseases. However, the commonly used CT/MRI-based methods are time consuming and, moreover, costly. Therefore we propose an alternative approach to brain perfusion imaging by means of ultrasonography. In spite of the low signal/noise-ratio of transcranial ultrasound and the high impedance of the skull, flow images of cerebral blood flow can be derived by capturing the kinetics of appropriate contrast agents by harmonic ultrasound image sequences. In this paper we propose three different methods for human brain perfusion imaging, each of which yielding flow images indicating the status of the patient's cerebral microcirculation by visualising local flow parameters. Bolus harmonic imaging (BHI) displays the flow kinetics of bolus injections, while replenishment (RHI) and diminution harmonic imaging (DHI) compute flow characteristics from contrast agent continuous infusions. RHI measures the contrast agents kinetics in the influx phase and DHI displays the diminution kinetics of the contrast agent acquired from the decay phase. In clinical studies, BHI- and RHI-parameter images were found to represent comprehensive and reproducible distributions of physiological cerebral blood flow. For DHI it is shown, that bubble destruction and hence perfusion phenomena principally can be displayed. Generally, perfusion harmonic imaging enables reliable and fast bedside imaging of human brain perfusion. Due to its cost efficiency it complements cerebrovascular diagnostics by established CT/MRI-based methods.

  17. Frequency multiplied harmonic gyrotron-traveling-wave-tube amplifier

    SciTech Connect

    Choi, J.J.; Ganguly, A.K.; Armstrong, C.M. )

    1994-06-01

    Numerical simulations of a [ital W]-band two-stage tapered, frequency multiplied gyrotron- traveling-wave-tube amplifier are reported. Unlike conventional harmonic gyrodevices, a drive signal at the fundamental harmonic frequency is injected in the first stage for beam modulation, and amplified output radiation is extracted from the third harmonic cyclotron resonance interaction. Numerical results show that broadband millimeter wave radiation is obtained with an efficiency of 10%--15%, a gain of [similar to]30 dB, and an instantaneous bandwidth of [similar to]10% at a center frequency of 95 GHz for [Delta][ital v][sub [ital z

  18. Frequency-resolved optical grating using third-harmonic generation

    SciTech Connect

    Tsang, T.; Krumbuegel, M.A.; Delong, K.W.

    1995-12-01

    We demonstrate the first frequency-resolved optical gating measurement of an laser oscillator without the time ambiguity using third-harmonic generation. The experiment agrees well with the phase-retrieved spectrograms.

  19. Phase responses of harmonics reflected from radio-frequency electronics

    NASA Astrophysics Data System (ADS)

    Mazzaro, Gregory J.; McGowan, Sean F.; Gallagher, Kyle A.; Sherbondy, Kelly D.; Martone, Anthony F.; Narayanan, Ram M.

    2016-05-01

    The phase responses of nonlinear-radar targets illuminated by stepped frequencies are studied. Data is presented for an experimental radar and two commercial electronic targets at short standoff ranges. The amplitudes and phases of harmonics generated by each target at each frequency are captured over a 100-MHz-wide transmit band. As in the authors' prior work, target detection is demonstrated by receiving at least one harmonic of at least one transmit frequency. In the present work, experiments confirm that the phase of a harmonic reflected from a radio-frequency electronic target at a standoff distance is linear versus frequency. Similar to traditional wideband radar, the change of the reflected phase with respect to frequency indicates the range to the nonlinear target.

  20. Plane gyroklinotron at first and third harmonics of cyclotron frequency

    SciTech Connect

    Kurayev, A.A.; Lukashonok, D.V.; Sinitsyn, A.K. E-mail: timka86@gmail.com

    2011-07-01

    The results of gyroklinotron's parameters optimization for efficiency at f = 100 GHz with interaction on first and third harmonics of the cyclotron frequency are presented. The predicted electron gyroklinotron's efficiency reaches 70% on first harmonic and 40% on third harmonic. This is more than in usual gyrotron. Besides in contrast to usual gyrotron the width electron beam on radius of guiding centers of electron orbits in gyroklinotron may considerable exceed working wave length {lambda}. This allows to use in it considerable more power of electron beams EB then in usual gyrotron. (author)

  1. SHG nanoprobes: advancing harmonic imaging in biology.

    PubMed

    Dempsey, William P; Fraser, Scott E; Pantazis, Periklis

    2012-05-01

    Second harmonic generating (SHG) nanoprobes have recently emerged as versatile and durable labels suitable for in vivo imaging, circumventing many of the inherent drawbacks encountered with classical fluorescent probes. Since their nanocrystalline structure lacks a central point of symmetry, they are capable of generating second harmonic signal under intense illumination - converting two photons into one photon of half the incident wavelength - and can be detected by conventional two-photon microscopy. Because the optical signal of SHG nanoprobes is based on scattering, rather than absorption as in the case of fluorescent probes, they neither bleach nor blink, and the signal does not saturate with increasing illumination intensity. When SHG nanoprobes are used to image live tissue, the SHG signal can be detected with little background signal, and they are physiologically inert, showing excellent long-term photostability. Because of their photophysical properties, SHG nanoprobes provide unique advantages for molecular imaging of living cells and tissues with unmatched sensitivity and temporal resolution. PMID:22392481

  2. Nonlinear propagation in ultrasonic fields: measurements, modelling and harmonic imaging.

    PubMed

    Humphrey, V F

    2000-03-01

    In high amplitude ultrasonic fields, such as those used in medical ultrasound, nonlinear propagation can result in waveform distortion and the generation of harmonics of the initial frequency. In the nearfield of a transducer this process is complicated by diffraction effects associated with the source. The results of a programme to study the nonlinear propagation in the fields of circular, focused and rectangular transducers are described, and comparisons made with numerical predictions obtained using a finite difference solution to the Khokhlov-Zabolotskaya-Kuznetsov (or KZK) equation. These results are extended to consider nonlinear propagation in tissue-like media and the implications for ultrasonic measurements and ultrasonic heating are discussed. The narrower beamwidths and reduced side-lobe levels of the harmonic beams are illustrated and the use of harmonics to form diagnostic images with improved resolution is described. PMID:10829672

  3. Investigation of plasma diagnostics using a dual frequency harmonic technique

    SciTech Connect

    Kim, Dong-Hwan; Kim, Young-Do; Cho, Sung-Won; Kim, Yu-Sin; Chung, Chin-Wook

    2014-09-07

    Plasma diagnostic methods using harmonic currents analysis of electrostatic probes were experimentally investigated to understand the differences in their measurement of the plasma parameters. When dual frequency voltage (ω{sub 1},ω{sub 2}) was applied to a probe, various harmonic currents (ω{sub 1}, 2ω{sub 1},ω{sub 2}, 2ω{sub 2},ω{sub 2}±ω{sub 1},ω{sub 2}±2ω{sub 1}) were generated due to the non-linearity of the probe sheath. The electron temperature can be obtained from the ratio of the two harmonics of the probe currents. According to the combinations of the two harmonics, the sensitivities in the measurement of the electron temperature differed, and this results in a difference of the electron temperature. From experiments and simulation, it is shown that this difference is caused by the systematic and random noise.

  4. A Novel Split-Waveguide Mount Design For MM and SubMM wave frequency multipliers and Harmonic Mixers

    NASA Technical Reports Server (NTRS)

    Raisanen, Anti V.; Choudhury, Debabani; Dengler, Robert J.; Oswald, John E.; Siegel, Peter H.

    1993-01-01

    A novel split-waveguide mount for millimeter and submillimeter wave frequency multipliers and harmonic mixers is presented. It consists of only two pieces, block halves, which are mirror images of each other.

  5. Multimode Directional Coupler for Utilization of Harmonic Frequencies from TWTAs

    NASA Technical Reports Server (NTRS)

    Simmons, Rainee N.; Wintucky, Edwin G.

    2013-01-01

    A novel waveguide multimode directional coupler (MDC) intended for the measurement and potential utilization of the second and higher order harmonic frequencies from high-power traveling wave tube amplifiers (TWTAs) has been successfully designed, fabricated, and tested. The design is based on the characteristic multiple propagation modes of the electrical and magnetic field components of electromagnetic waves in a rectangular waveguide. The purpose was to create a rugged, easily constructed, more efficient waveguide- based MDC for extraction and exploitation of the second harmonic signal from the RF output of high-power TWTs used for space communications. The application would be a satellitebased beacon source needed for Qband and V/W-band atmospheric propagation studies. The MDC could function as a CW narrow-band source or as a wideband source for study of atmospheric group delay effects on highdata- rate links. The MDC is fabricated from two sections of waveguide - a primary one for the fundamental frequency and a secondary waveguide for the second harmonic - that are joined together such that the second harmonic higher order modes are selectively coupled via precision- machined slots for propagation in the secondary waveguide. In the TWTA output waveguide port, both the fundamental and the second harmonic signals are present. These signals propagate in the output waveguide as the dominant and higher order modes, respectively. By including an appropriate mode selective waveguide directional coupler, such as the MDC presented here at the output of the TWTA, the power at the second harmonic can be sampled and amplified to the power level needed for atmospheric propagation studies. The important conclusions from the preliminary test results for the multimode directional coupler are: (1) the second harmonic (Ka-band) can be measured and effectively separated from the fundamental (Ku-band) with no coupling of the latter, (2) power losses in the fundamental frequency

  6. Observations of High Frequency Harmonics of the Ionospheric Alfven Resonator

    NASA Astrophysics Data System (ADS)

    Mann, Ian; Usanova, Maria; Bortnik, Jacob; Milling, David; Kale, Andy; Shao, Leo; Miles, David; Rae, I. Jonathan

    We present observations of high frequency harmonics of the ionospheric Alfven Resonator (IAR). These are seen in the form of spectral resonance structures (SRS) recorded by a ground-based search coil magnetometer sampling at 100 samples/s at the Ministik Lake station at L=4.2 within the expanded CARISMA magnetometer array. Previous observational studies have indicated that such SRS are typically confined to frequencies <~5 Hz with only several SRS harmonics being observed. We report the first observations of clear and discrete SRS, which we believe are harmonics of the IAR, and which extend to around 20 Hz in at least 10-12 clear SRS harmonics. We additionally demonstrate the utility of the Bortnik et al. (2007) auto-detection algorithm, designed for Pc1 wavepackets, for characterising the properties of the IAR. Our results also indicate that the cavity supporting SRS in the IAR at this time must be structured to support and trap much higher frequency IAR harmonics than previously assumed. This impacts the potential importance of the IAR for magnetosphere-ionosphere coupling, especially in relation to the impacts of incident Alfven waves on the ionosphere including Alfvenic aurora. Our observations also highlight the potential value of IAR observations for diagnosing the structure of the topside ionosphere, not least using the observed structure of the SRS. These are the first mid-latitude observations demonstrating that the IAR can extend to frequencies beyond those of the lowest few harmonics of the Schumann resonances - significantly suggesting the possibility that the Schumann resonance modes and the IAR may be coupled. The in-situ structure of the IAR is also examined by combining satellite data with conjugate measurements from the ground, and the impacts of the IAR for magnetosphere-ionosphere-thermosphere coupling examined.

  7. Excitation of electron Langmuir frequency harmonics in the solar atmosphere

    SciTech Connect

    Fomichev, V. V.; Fainshtein, S. M.; Chernov, G. P.

    2013-05-15

    An alternative mechanism for the excitation of electron Langmuir frequency harmonics as a result of the development of explosive instability in a weakly relativistic beam-plasma system in the solar atmosphere is proposed. The efficiency of the new mechanism as compared to the previously discussed ones is analyzed.

  8. Multi-Frequency Harmonics Technique for HIFU Tissue Treatment

    NASA Astrophysics Data System (ADS)

    Rybyanets, Andrey N.; Lugovaya, Maria A.; Rybyanets, Anastasia A.

    2010-03-01

    New technique for enhancing of tissue lysis and enlarging treatment volume during one HIFU sonification is proposed. The technique consists in simultaneous or alternative (at optimal repetition frequency) excitation of single element HIFU transducer on a frequencies corresponding to odd natural harmonics of piezoceramic element at ultrasound energy levels sufficient for producing cavitational, thermal or mechanical damage of fat cells at each of aforementioned frequencies. Calculation and FEM modeling of transducer vibrations and acoustic field patterns for different frequencies sets were performed. Acoustic pressure in focal plane was measured in water using calibrated hydrophone and 3D acoustic scanning system. In vitro experiments on different tissues and phantoms confirming the advantages of multifrequency harmonic method were performed.

  9. Lens-less surface second harmonic imaging.

    PubMed

    Sly, Krystal L; Nguyen, Trang T; Conboy, John C

    2012-09-24

    Lens-less surface second harmonic generation imaging (SSHGI) is used to image an SHG active molecule, (S)-(+)-1,1'-bi-2-naphthol (SBN), incorporated into a lipid bilayer patterned with the 1951 United States Air Force resolution test target. Data show the coherent plane-wave nature of SHG allows direct imaging without the aid of a lens system. Lens-less SSHGI readily resolves line-widths as small as 223 μm at an object-image distance of 7.6 cm and line-widths of 397 μm at distances as far as 30 cm. Lens-less SSHGI simplifies the detection method, raises photon collection efficiency, and expands the field-of-view. These advantages allow greater throughput and make lens-less SSHGI a potentially valuable detection method for biosensors and medical diagnostics. PMID:23037346

  10. Harmonic Motion Imaging (HMI) for Tumor Imaging and Treatment Monitoring

    PubMed Central

    Maleke, Caroline; Vappou, Jonathan

    2014-01-01

    Palpation is an established screening procedure for the detection of several superficial cancers including breast, thyroid, prostate, and liver tumors through both self and clinical examinations. This is because solid masses typically have distinct stiffnesses compared to the surrounding normal tissue. In this paper, the application of Harmonic Motion Imaging (HMI) for tumor detection based on its stiffness as well as its relevance in thermal treatment is reviewed. HMI uses a focused ultrasound (FUS) beam to generate an oscillatory acoustic radiation force for an internal, non-contact palpation to internally estimate relative tissue hardness. HMI studies have dealt with the measurement of the tissue dynamic motion in response to an oscillatory acoustic force at the same frequency, and have been shown feasible in simulations, phantoms, ex vivo human and bovine tissues as well as animals in vivo. Using an FUS beam, HMI can also be used in an ideal integration setting with thermal ablation using high-intensity focused ultrasound (HIFU), which also leads to an alteration in the tumor stiffness. In this paper, a short review of HMI is provided that encompasses the findings in all the aforementioned areas. The findings presented herein demonstrate that the HMI displacement can accurately depict the underlying tissue stiffness, and the HMI image of the relative stiffness could accurately detect and characterize the tumor or thermal lesion based on its distinct properties. HMI may thus constitute a non-ionizing, cost-efficient and reliable complementary method for noninvasive tumor detection, localization, diagnosis and treatment monitoring. PMID:25364321

  11. Contrast and harmonic imaging improves accuracy and efficiency of novice readers for dobutamine stress echocardiography

    NASA Technical Reports Server (NTRS)

    Vlassak, Irmien; Rubin, David N.; Odabashian, Jill A.; Garcia, Mario J.; King, Lisa M.; Lin, Steve S.; Drinko, Jeanne K.; Morehead, Annitta J.; Prior, David L.; Asher, Craig R.; Klein, Allan L.; Thomas, James D.

    2002-01-01

    of harmonic imaging reduces the frequency of nondiagnostic wall segments.

  12. Optical Frequency Domain Imaging

    NASA Astrophysics Data System (ADS)

    Bouma, Brett E.; Tearney, Guillermo J.; Vakoc, Benjamin; Yun, Seok Hyun

    In this chapter, we discuss a frequency-domain approach, optical frequency-domain imaging (OFDI), which is based on optical frequency-domain reflectometry and uses a wavelength-swept laser and standard single-element photodetectors. The chapter begins with an overview of the fundamental aspects of the technology, including the detected signal, sensitivity, depth range, and resolution, and then goes on to discuss specific component technologies including the light source, interferometer and acquisition electronics, and image processing. The final section of the chapter provides a brief glimpse at some of the biomedical applications that most directly take advantage of the improved speed and sensitivity of OFDI.

  13. Critical frequency control in harmonic quantum Brownian motion

    NASA Astrophysics Data System (ADS)

    Giraldi, Filippo; Petruccione, Francesco

    2013-01-01

    The dissipative effects of a quantum harmonic oscillator, initially set in a coherent state and linearly coupled to a continuous distribution of frequency modes, are analyzed over long time scales in relation to the behavior of the spectral density near an arbitrary band gap, arbitrarily shaped at the higher frequencies. The reservoir is initially set either in the vacuum state or in continuous distributions of coherent states. These distributions are arbitrarily shaped at high frequencies and structured in sub- or super-ohmic configurations near an arbitrary band gap frequency. Similarly to certain decoherence processes of a qubit, critical conditions emerge, such that arbitrarily slow inverse power law relaxations of the expectation values of the observables, are obtained by approaching the boundary between the sub- and the super-ohmic regimes. Also, in such critical conditions, a trapping of the number of excitations appears in the super-ohmic regime. The technique of critical frequency control, emerging in the scenario of the environment-induced decoherence of a qubit via the reservoir engineering approach, is extended to the harmonic quantum Brownian motion.

  14. Non-Harmonic Analysis Applied to Optical Coherence Tomography Imaging

    NASA Astrophysics Data System (ADS)

    Cao, Xu; Uchida, Tetsuya; Hirobayashi, Shigeki; Chong, Changho; Morosawa, Atsushi; Totsuka, Koki; Suzuki, Takuya

    2012-02-01

    A new processing technique called non-harmonic analysis (NHA) is proposed for optical coherence tomography (OCT) imaging. Conventional Fourier-domain OCT employs the discrete Fourier transform (DFT), which depends on the window function and length. The axial resolution of the OCT image, calculated by using DFT, is inversely proportional to the full width at half maximum (FWHM) of the wavelength range. The FWHM of wavelength range is limited by the sweeping range of the source in swept-source OCT and it is limited by the number of CCD pixels in spectral-domain OCT. However, the NHA process does not have such constraints; NHA can resolve high frequencies irrespective of the window function and the frame length of the sampled data. In this study, the NHA process is described and it is applied to OCT imaging. It is compared with OCT images based on the DFT. To demonstrate the benefits of using NHA for OCT, we perform OCT imaging with NHA of an onion skin. The results reveal that NHA can achieve an image resolution equivalent that of a 100-nm sweep range using a significantly reduced wavelength range. They also reveal the potential of using this technique to achieve high-resolution imaging without using a broadband source. However, the long calculation times required for NHA must be addressed if it is to be used in clinical applications.

  15. Bond length, dipole moment, and harmonic frequency of CO

    NASA Technical Reports Server (NTRS)

    Barnes, Leslie A.; Liu, Bowen; Lindh, Roland

    1993-01-01

    A detailed comparison of some properties of CO is given, at the modified coupled-pair functional, single and double excitation coupled-cluster (CCSD), and CCSD(T) levels of theory (including a perturbational estimate for connected triple excitations), using a variety of basis sets. With very large one-particle basis sets, the CCSD(T) method gives excellent results for the bond distance, dipole moment, and harmonic frequency of CO. In a (6s 5p 4d 3f 2g 1h) + (1s 1p 1d) basis set, the bond distance is about 0.005a0 too large, the dipole moment about 0.005 a.u. too small, and the frequency about 6/cm too small, when compared with experimental results.

  16. Balancing Vibrations at Harmonic Frequencies by Injecting Harmonic Balancing Signals into the Armature of a Linear Motor/Alternator Coupled to a Stirling Machine

    NASA Technical Reports Server (NTRS)

    Holliday, Ezekiel S. (Inventor)

    2014-01-01

    Vibrations at harmonic frequencies are reduced by injecting harmonic balancing signals into the armature of a linear motor/alternator coupled to a Stirling machine. The vibrations are sensed to provide a signal representing the mechanical vibrations. A harmonic balancing signal is generated for selected harmonics of the operating frequency by processing the sensed vibration signal with adaptive filter algorithms of adaptive filters for each harmonic. Reference inputs for each harmonic are applied to the adaptive filter algorithms at the frequency of the selected harmonic. The harmonic balancing signals for all of the harmonics are summed with a principal control signal. The harmonic balancing signals modify the principal electrical drive voltage and drive the motor/alternator with a drive voltage component in opposition to the vibration at each harmonic.

  17. Interpretations of Frequency Domain Analyses of Neural Entrainment: Periodicity, Fundamental Frequency, and Harmonics.

    PubMed

    Zhou, Hong; Melloni, Lucia; Poeppel, David; Ding, Nai

    2016-01-01

    Brain activity can follow the rhythms of dynamic sensory stimuli, such as speech and music, a phenomenon called neural entrainment. It has been hypothesized that low-frequency neural entrainment in the neural delta and theta bands provides a potential mechanism to represent and integrate temporal information. Low-frequency neural entrainment is often studied using periodically changing stimuli and is analyzed in the frequency domain using the Fourier analysis. The Fourier analysis decomposes a periodic signal into harmonically related sinusoids. However, it is not intuitive how these harmonically related components are related to the response waveform. Here, we explain the interpretation of response harmonics, with a special focus on very low-frequency neural entrainment near 1 Hz. It is illustrated why neural responses repeating at f Hz do not necessarily generate any neural response at f Hz in the Fourier spectrum. A strong neural response at f Hz indicates that the time scales of the neural response waveform within each cycle match the time scales of the stimulus rhythm. Therefore, neural entrainment at very low frequency implies not only that the neural response repeats at f Hz but also that each period of the neural response is a slow wave matching the time scale of a f Hz sinusoid. PMID:27375465

  18. Interpretations of Frequency Domain Analyses of Neural Entrainment: Periodicity, Fundamental Frequency, and Harmonics

    PubMed Central

    Zhou, Hong; Melloni, Lucia; Poeppel, David; Ding, Nai

    2016-01-01

    Brain activity can follow the rhythms of dynamic sensory stimuli, such as speech and music, a phenomenon called neural entrainment. It has been hypothesized that low-frequency neural entrainment in the neural delta and theta bands provides a potential mechanism to represent and integrate temporal information. Low-frequency neural entrainment is often studied using periodically changing stimuli and is analyzed in the frequency domain using the Fourier analysis. The Fourier analysis decomposes a periodic signal into harmonically related sinusoids. However, it is not intuitive how these harmonically related components are related to the response waveform. Here, we explain the interpretation of response harmonics, with a special focus on very low-frequency neural entrainment near 1 Hz. It is illustrated why neural responses repeating at f Hz do not necessarily generate any neural response at f Hz in the Fourier spectrum. A strong neural response at f Hz indicates that the time scales of the neural response waveform within each cycle match the time scales of the stimulus rhythm. Therefore, neural entrainment at very low frequency implies not only that the neural response repeats at f Hz but also that each period of the neural response is a slow wave matching the time scale of a f Hz sinusoid. PMID:27375465

  19. Optical coherence tomography imaging based on non-harmonic analysis

    NASA Astrophysics Data System (ADS)

    Cao, Xu; Hirobayashi, Shigeki; Chong, Changho; Morosawa, Atsushi; Totsuka, Koki; Suzuki, Takuya

    2009-11-01

    A new processing technique called Non-Harmonic Analysis (NHA) is proposed for OCT imaging. Conventional Fourier-Domain OCT relies on the FFT calculation which depends on the window function and length. Axial resolution is counter proportional to the frame length of FFT that is limited by the swept range of the swept source in SS-OCT, or the pixel counts of CCD in SD-OCT degraded in FD-OCT. However, NHA process is intrinsically free from this trade-offs; NHA can resolve high frequency without being influenced by window function or frame length of sampled data. In this study, NHA process is explained and applied to OCT imaging and compared with OCT images based on FFT. In order to validate the benefit of NHA in OCT, we carried out OCT imaging based on NHA with the three different sample of onion-skin,human-skin and pig-eye. The results show that NHA process can realize practical image resolution that is equivalent to 100nm swept range only with less than half-reduced wavelength range.

  20. Measuring amplitudes of harmonics and combination frequencies in variable stars

    NASA Astrophysics Data System (ADS)

    Bellinger, E. P.; Wysocki, D.; Kanbur, S. M.

    2016-05-01

    Discoveries of RR Lyrae and Cepheid variable stars with multiple modes of pulsation have increased tremendously in recent years. The Fourier spectra of these stars can be quite complicated due to the large number of combination frequencies that can exist between their modes. As a result, light- curve fits to these stars often suffer from undesirable ringing effects that arise from noisy observations and poor phase coverage. These non-physical overfitting artifacts also occur when fitting the harmonics of single-mode stars. Here we present a new method for fitting light curves that is much more robust against these effects. We prove that the amplitude measurement problem is very difficult (NP-hard) and provide a heuristic algorithm for solving it quickly and accurately.

  1. Qualitative and quantitative effects of harmonic echocardiographic imaging on endocardial edge definition and side-lobe artifacts

    NASA Technical Reports Server (NTRS)

    Rubin, D. N.; Yazbek, N.; Garcia, M. J.; Stewart, W. J.; Thomas, J. D.

    2000-01-01

    Harmonic imaging is a new ultrasonographic technique that is designed to improve image quality by exploiting the spontaneous generation of higher frequencies as ultrasound propagates through tissue. We studied 51 difficult-to-image patients with blinded side-by-side cineloop evaluation of endocardial border definition by harmonic versus fundamental imaging. In addition, quantitative intensities from cavity versus wall were compared for harmonic versus fundamental imaging. Harmonic imaging improved left ventricular endocardial border delineation over fundamental imaging (superior: harmonic = 71.1%, fundamental = 18.7%; similar: 10.2%; P <.001). Quantitative analysis of 100 wall/cavity combinations demonstrated brighter wall segments and more strikingly darker cavities during harmonic imaging (cavity intensity on a 0 to 255 scale: fundamental = 15.6 +/- 8.6; harmonic = 6.0 +/- 5.3; P <.0001), which led to enhanced contrast between the wall and cavity (1.89 versus 1.19, P <.0001). Harmonic imaging reduces side-lobe artifacts, resulting in a darker cavity and brighter walls, thereby improving image contrast and endocardial delineation.

  2. Driving an Active Vibration Balancer to Minimize Vibrations at the Fundamental and Harmonic Frequencies

    NASA Technical Reports Server (NTRS)

    Holliday, Ezekiel S. (Inventor)

    2014-01-01

    Vibrations of a principal machine are reduced at the fundamental and harmonic frequencies by driving the drive motor of an active balancer with balancing signals at the fundamental and selected harmonics. Vibrations are sensed to provide a signal representing the mechanical vibrations. A balancing signal generator for the fundamental and for each selected harmonic processes the sensed vibration signal with adaptive filter algorithms of adaptive filters for each frequency to generate a balancing signal for each frequency. Reference inputs for each frequency are applied to the adaptive filter algorithms of each balancing signal generator at the frequency assigned to the generator. The harmonic balancing signals for all of the frequencies are summed and applied to drive the drive motor. The harmonic balancing signals drive the drive motor with a drive voltage component in opposition to the vibration at each frequency.

  3. Harmonic source wavefront aberration correction for ultrasound imaging

    PubMed Central

    Dianis, Scott W.; von Ramm, Olaf T.

    2011-01-01

    A method is proposed which uses a lower-frequency transmit to create a known harmonic acoustical source in tissue suitable for wavefront correction without a priori assumptions of the target or requiring a transponder. The measurement and imaging steps of this method were implemented on the Duke phased array system with a two-dimensional (2-D) array. The method was tested with multiple electronic aberrators [0.39π to 1.16π radians root-mean-square (rms) at 4.17 MHz] and with a physical aberrator 0.17π radians rms at 4.17 MHz) in a variety of imaging situations. Corrections were quantified in terms of peak beam amplitude compared to the unaberrated case, with restoration between 0.6 and 36.6 dB of peak amplitude with a single correction. Standard phantom images before and after correction were obtained and showed both visible improvement and 14 dB contrast improvement after correction. This method, when combined with previous phase correction methods, may be an important step that leads to improved clinical images. PMID:21303031

  4. Multi-Channel Microstrip Transceiver Arrays Using Harmonics for High Field MR Imaging in Humans

    PubMed Central

    Wu, Bing; Wang, Chunsheng; Lu, Jonathan; Pang, Yong; Nelson, Sarah J; Vigneron, Daniel B; Zhang, Xiaoliang

    2012-01-01

    RF transceiver array design using primary and higher order harmonics for in-vivo parallel MR imaging and spectroscopic imaging is proposed. The improved electromagnetic decoupling performance, unique magnetic field distributions and high-frequency operation capabilities of higher-order harmonics of resonators would benefit transceiver arrays for parallel MRI, especially for ultrahigh field parallel MRI. To demonstrate this technique, microstrip transceiver arrays using first and second harmonic resonators were developed for human head parallel imaging at 7T. Phantom and human head images were acquired and evaluated using the GRAPPA reconstruction algorithm. The higher-order harmonic transceiver array design technique was also assessed numerically using FDTD simulation. Compared with regular primary-resonance transceiver designs, the proposed higher-order harmonic technique provided an improved g-factor and increased decoupling among resonant elements without using dedicated decoupling circuits, which would potentially lead to a better parallel imaging performance and ultimately faster and higher quality imaging. The proposed technique is particularly suitable for densely spaced transceiver array design where the increased mutual inductance among the elements becomes problematic. In addition, it also provides a simple approach to readily upgrade the channels of a conventional primary resonator microstrip array to a larger number for faster imaging. PMID:21878410

  5. Harmonic Spatial Coherence Imaging: An Ultrasonic Imaging Method Based on Backscatter Coherence

    PubMed Central

    Dahl, Jeremy J; Jakovljevic, Marko; Pinton, Gianmarco F.; Trahey, Gregg E.

    2012-01-01

    HSCI and SLSC imaging less sensitive to clutter because it has low spatial coherence. The method is based on the coherence of the second harmonic backscatter. Because the same signals that are used to construct harmonic B-mode images are also used to construct HSCI images, the benefits obtained with harmonic imaging are also applicable to HSCI. Harmonic imaging has been the primary tool for suppressing clutter in diagnostic ultrasound imaging, however second harmonic echoes are not necessarily immune to the effects of clutter. HSCI and SLSC imaging are less sensitive to clutter because it has low spatial coherence. Harmonic Spatial Coherence Imaging shows favorable imaging characteristics such as improved contrast-to-noise ratio (CNR), improved speckle signal-to-noise ratio (SNR), and better delineation of borders and other structures compared to fundamental and harmonic B-mode imaging. CNRs of up to 1.9 were obtained from in vivo imaging of human cardiac tissue with HSCI, compared to 0.6, 0.9, and 1.5 in fundamental B-mode, harmonic B-mode, and SLSC imaging, respectively. In vivo experiments in human liver tissue demonstrated SNRs of up to 3.4 for HSCI compared to 1.9 for harmonic B-mode. Nonlinear simulations of a heart chamber model were consistent with the in vivo experiments. PMID:22547276

  6. Invariant quaternion radial harmonic Fourier moments for color image retrieval

    NASA Astrophysics Data System (ADS)

    Xiang-yang, Wang; Wei-yi, Li; Hong-ying, Yang; Pan-pan, Niu; Yong-wei, Li

    2015-03-01

    Moments and moment invariants have become a powerful tool in image processing owing to their image description capability and invariance property. But, conventional methods are mainly introduced to deal with the binary or gray-scale images, and the only approaches for color image always have poor color image description capability. Based on radial harmonic Fourier moments (RHFMs) and quaternion, we introduced the quaternion radial harmonic Fourier moments (QRHFMs) for representing color images in this paper, which can be seen as the generalization of RHFMs for gray-level images. It is shown that the QRHFMs can be obtained from the RHFMs of each color channel. We derived and analyzed the rotation, scaling, and translation (RST) invariant property of QRHFMs. We also discussed the problem of color image retrieval using invariant QRHFMs. Experimental results are provided to illustrate the efficiency of the proposed color image representation.

  7. Second-harmonic generation imaging of metal nano-objects with cylindrical vector beams.

    PubMed

    Bautista, Godofredo; Huttunen, Mikko J; Mäkitalo, Jouni; Kontio, Juha M; Simonen, Janne; Kauranen, Martti

    2012-06-13

    We introduce an imaging technique based on second-harmonic generation with cylindrical vector beams that is extremely sensitive to three-dimensional orientation and nanoscale morphology of metal nano-objects. Our experiments and second-harmonic field calculations based on frequency-domain boundary element method are in very good agreement. The technique provides contrast for structural features that cannot be resolved by linear techniques or conventional states of polarization and shows great potential for simple and cost-effective far-field optical imaging in plasmonics. PMID:22587307

  8. Imaging leukocytes in vivo with third harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Tsai, Cheng-Kun; Chen, Chien-Kuo; Chen, Yu-Shing; Wu, Pei-Chun; Hsieh, Tsung-Yuan; Liu, Han-Wen; Yeh, Chiou-Yueh; Lin, Win-Li; Chia, Jean-San; Liu, Tzu-Ming

    2013-02-01

    Without a labeling, we demonstrated that lipid granules in leukocytes have distinctive third harmonic generation (THG) contrast. Excited by a 1230nm femtosecond laser, THG signals were generated at a significantly higher level in neutrophils than other mononuclear cells, whereas signals in agranular lymphocytes were one order smaller. These characteristic THG features can also be observed in vivo to trace the newly recruited leukocytes following lipopolysaccharide (LPS) challenge. Furthermore, using video-rate THG microscopy, we also captured images of blood cells in human capillaries. Quite different from red-blood-cells, every now and then, round and granule rich blood cells with strong THG contrast appeared in circulation. The corresponding volume densities in blood, evaluated from their frequencies of appearance and the velocity of circulation, fall within the physiological range of human white blood cell counts. These results suggested that labeling-free THG imaging may provide timely tracing of leukocyte movement and hematology inspection without disturbing the normal cellular or physiological status.

  9. Confocal Imaging of Biological Tissues Using Second Harmonic Generation

    SciTech Connect

    Kim, B-M.; Stoller, P.; Reiser, K.; Eichler, J.; Yan, M.; Rubenchik, A.; Da Silva, L.

    2000-03-06

    A confocal microscopy imaging system was devised to selectively detect Second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

  10. Quantitative viscoelastic parameters measured by harmonic motion imaging.

    PubMed

    Vappou, Jonathan; Maleke, Caroline; Konofagou, Elisa E

    2009-06-01

    Quantifying the mechanical properties of soft tissues remains a challenging objective in the field of elasticity imaging. In this work, we propose an ultrasound-based method for quantitatively estimating viscoelastic properties, using the amplitude-modulated harmonic motion imaging (HMI) technique. In HMI, an oscillating acoustic radiation force is generated inside the medium by using focused ultrasound and the resulting displacements are measured using an imaging transducer. The proposed approach is a two-step method that uses both the properties of the propagating shear wave and the phase shift between the applied stress and the measured strain in order to infer to the shear storage (G') and shear loss modulus (G''), which refer to the underlying tissue elasticity and viscosity, respectively. The proposed method was first evaluated on numerical phantoms generated by finite-element simulations, where a very good agreement was found between the input and the measured values of G' and G''. Experiments were then performed on three soft tissue-mimicking gel phantoms. HMI measurements were compared to rotational rheometry (dynamic mechanical analysis), and very good agreement was found at the only overlapping frequency (10 Hz) in the estimate of the shear storage modulus G' (14% relative error, averaged p-value of 0.34), whereas poorer agreement was found in G'' (55% relative error, averaged p-value of 0.0007), most likely due to the significantly lower values of G'' of the gel phantoms, posing thus a greater challenge in the sensitivity of the method. Nevertheless, this work proposes an original model-independent ultrasound-based elasticity imaging method that allows for direct, quantitative estimation of tissue viscoelastic properties, together with a validation against mechanical testing. PMID:19454785

  11. A Novel Multimode Waveguide Coupler for Accurate Power Measurement of Traveling Wave Tube Harmonic Frequencies

    NASA Technical Reports Server (NTRS)

    Wintucky, Edwin G.; Simons, Rainee N.

    2014-01-01

    This paper presents the design, fabrication and test results for a novel waveguide multimode directional coupler (MDC). The coupler fabricated from two dissimilar waveguides is capable of isolating the power at the second harmonic frequency from the fundamental power at the output port of a traveling-wave tube (TWT). In addition to accurate power measurements at harmonic frequencies, a potential application of the MDC is in the design of a beacon source for atmospheric propagation studies at millimeter-wave frequencies.

  12. Dual-frequency transducer for nonlinear contrast agent imaging.

    PubMed

    Guiroy, Axel; Novell, Anthony; Ringgaard, Erling; Lou-Moeller, Rasmus; Grégoire, Jean-Marc; Abellard, André-Pierre; Zawada, Tomasz; Bouakaz, Ayache; Levassort, Franck

    2013-12-01

    Detection of high-order nonlinear components issued from microbubbles has emerged as a sensitive method for contrast agent imaging. Nevertheless, the detection of these high-frequency components, including the third, fourth, and fifth harmonics, remains challenging because of the lack of transducer sensitivity and bandwidth. In this context, we propose a new design of imaging transducer based on a simple fabrication process for high-frequency nonlinear imaging. The transducer is composed of two elements: the outer low-frequency (LF) element was centered at 4 MHz and used in transmit mode, whereas the inner high-frequency (HF) element centered at 14 MHz was used in receive mode. The center element was pad-printed using a lead zirconate titanate (PZT) paste. The outer element was molded using a commercial PZT, and curved porous unpoled PZT was used as backing. Each piezoelectric element was characterized to determine the electromechanical performance with thickness coupling factor around 45%. After the assembly of the two transducer elements, hydrophone measurements (electroacoustic responses and radiation patterns) were carried out and demonstrated a large bandwidth (70% at -3 dB) of the HF transducer. Finally, the transducer was evaluated for contrast agent imaging using contrast agent microbubbles. The results showed that harmonic components (up to the sixth harmonic) of the microbubbles were successfully detected. Moreover, images from a flow phantom were acquired and demonstrated the potential of the transducer for high-frequency nonlinear contrast imaging. PMID:24297028

  13. Corneal imaging by second and third harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Brocas, Arnaud; Jay, Louis; Mottay, Eric; Brunette, Isabelle; Ozaki, Tsuneyuki

    2008-02-01

    Advanced imaging methods are essential tools for improved outcome of refractive surgery. Second harmonic generation (SHG) and third harmonic generation (THG) microscopy are noninvasive high-resolution imaging methods, which can discriminate the different layers of the cornea, thus having strong impact on the outcome of laser surgery. In this work, we use an Ytterbium femtosecond laser as the laser source, the longer wavelength of which reduces scattering, and allows simultaneous SHG and THG imaging. We present SHG and THG images and profiles of pig corneas that clearly show the anterior surface of the cornea, the entry in the stroma and its end, and the posterior surface of the cornea. These observations allow localizing the epithelium, the stroma and the endothelium. Other experiments give information about the structure and cytology of the corneal layers.

  14. Radio-frequency excitation of harmonic microwave radiation from a Penning reflex discharge

    SciTech Connect

    Tate, J.P.; Wharton, C.B. )

    1993-04-01

    Experimental results on multiple-harmonic emission at 8.8 GHz from a Penning reflex discharge (PRD) are reported. Observations of the frequency spectra of microwave emission showed copius harmonic generation of frequencies having two completely different origins: (1) spontaneously excited high harmonics of the electron cyclotron frequency and (2) high harmonics of the frequency of an injected signal independent of the magnetic field strength, a phenomenon reported here for the first time. For spontaneous harmonic emission there was a current threshold, whose magnitude depended on gas pressure and magnetic field strength. When a signal was injected, however, high harmonics (up to the 18th) could be seen at discharge currents well below this threshold value. Comparisons between the two types of radiation are made and discussion of possible mechanisms is provided. It is concluded that the coupling efficiency of the radio-frequency (rf)-excited emission is dependent on the relationship between the rf drive frequency and the electron cyclotron frequency. Finite Larmor radius effects may also influence this coupling. The plasma sheath size will also be a factor in the transfer of energy from the probe to the bulk plasma. Results which seek to elucidate these effects are presented.

  15. Harmonic Frequency Lowering: Effects on the Perception of Music Detail and Sound Quality.

    PubMed

    Kirchberger, Martin; Russo, Frank A

    2016-01-01

    A novel algorithm for frequency lowering in music was developed and experimentally tested in hearing-impaired listeners. Harmonic frequency lowering (HFL) combines frequency transposition and frequency compression to preserve the harmonic content of music stimuli. Listeners were asked to make judgments regarding detail and sound quality in music stimuli. Stimuli were presented under different signal processing conditions: original, low-pass filtered, HFL, and nonlinear frequency compressed. Results showed that participants reported perceiving the most detail in the HFL condition. In addition, there was no difference in sound quality across conditions. PMID:26834122

  16. Frequency-resolved optical grating using surface third-harmonic generation

    SciTech Connect

    Tsang, T.; Krumbuegel, M.A.; Delong, K.W.; Fittinghoff, D.N.; Trebino, R.

    1995-11-01

    We demonstrate the frequency-resolved optical grating technique using third-harmonic generation on the surface of a cover glass with ultra-short optical pulses and compare that with the phase-retrieved spectrogram.

  17. Harmonic Motion Microwave Doppler Imaging method for breast tumor detection.

    PubMed

    Top, Can Barıs; Tafreshi, Azadeh Kamali; Gençer, Nevzat G

    2014-01-01

    Harmonic Motion Microwave Doppler Imaging (HMMDI) method is recently proposed as a non-invasive hybrid breast imaging technique for tumor detection. The acquired data depend on acoustic, elastic and electromagnetic properties of the tissue. The potential of the method is analyzed with simulation studies and phantom experiments. In this paper, the results of these studies are summarized. It is shown that HMMDI method has a potential to detect malignancies inside fibro-glandular tissue. PMID:25571382

  18. Storage ring free electron laser dynamics in presence of an auxiliary harmonic radio frequency cavity

    NASA Astrophysics Data System (ADS)

    Thomas, C. A.; Botman, J. I. M.; Bruni, C.; Orlandi, G.; de Ninno, G.; Garzella, D.; Couprie, M. E.

    2005-01-01

    In a Storage Ring Free Electron Laser (SRFEL) there is a strong interdependence between the laser beam and the electron beam from which the laser is generated. The Super ACO storage ring has a second Radio Frequency (RF) cavity at the 5th harmonic of the main RF cavity. It is used to shorten the bunch length, thereby enhancing the laser gain. Employing this RF harmonic cavity instabilities are observed with a strong effect on both the laser radiation properties and the electron beam behaviour. In this paper, we first present beam characteristics of Super-ACO as influenced by the harmonic cavity, and the instabilities of the beam due to this RF cavity. Then we discuss the FEL properties in presence of the harmonic RF cavity. In general the harmonic cavity functions as intended, and it is observed that the laser suppresses the instabilities caused by the harmonic cavity in the absence of the FEL.

  19. Imaging Collagen Orientation Using Polarization-Modulated Second Harmonic Generation

    SciTech Connect

    Stoller, P; Celliers, P M; Reiser, K M; Rubenchik, A M

    2002-01-10

    We use polarization-modulated second harmonic generation to image fiber orientation in collagen tissues, with an axial resolution of about 10 {micro}m and a transverse resolution of up to 1 {micro}m. A linearly polarized ultra-short pulse (200 fs) Ti:Sapphire laser beam is modulated using an electro-optic modulator and quarter-wave plate combination and focused onto a translation stage mounted sample using a microscope objective. The generated second harmonic light is collected using a photomultiplier tube and demodulated using phase sensitive detection to obtain signal intensity and fiber orientation information. In order to obtain second harmonic generation images of different types of collagen organization, we analyze several different tissues, including rat-tail tendon, mouse aorta, mouse fibrotic liver, and porcine skin. We can use our technique to image fibrotic tissue in histological sections of damaged liver and to identify burned tissue in porcine skin to a depth of a few hundred microns. Polarization-modulated second harmonic generation potentially could be a useful clinical technique for diagnosing collagen related disease or damage, especially in the skin.

  20. Analysis and measurement of the modulation transfer function of harmonic shear wave induced phase encoding imaging.

    PubMed

    McAleavey, Stephen A

    2014-05-01

    Shear wave induced phase encoding (SWIPE) imaging generates ultrasound backscatter images of tissue-like elastic materials by using traveling shear waves to encode the lateral position of the scatters in the phase of the received echo. In contrast to conventional ultrasound B-scan imaging, SWIPE offers the potential advantages of image formation without beam focusing or steering from a single transducer element, lateral resolution independent of aperture size, and the potential to achieve relatively high lateral resolution with low frequency ultrasound. Here a Fourier series description of the phase modulated echo signal is developed, demonstrating that echo harmonics at multiples of the shear wave frequency reveal target k-space data at identical multiples of the shear wavenumber. Modulation transfer functions of SWIPE imaging systems are calculated for maximum shear wave acceleration and maximum shear constraints, and compared with a conventionally focused aperture. The relative signal-to-noise ratio of the SWIPE method versus a conventionally focused aperture is found through these calculations. Reconstructions of wire targets in a gelatin phantom using 1 and 3.5 MHz ultrasound and a cylindrical shear wave source are presented, generated from the fundamental and second harmonic of the shear wave modulation frequency, demonstrating weak dependence of lateral resolution with ultrasound frequency. PMID:24815265

  1. Analysis and measurement of the modulation transfer function of harmonic shear wave induced phase encoding imaging

    PubMed Central

    McAleavey, Stephen A.

    2014-01-01

    Shear wave induced phase encoding (SWIPE) imaging generates ultrasound backscatter images of tissue-like elastic materials by using traveling shear waves to encode the lateral position of the scatters in the phase of the received echo. In contrast to conventional ultrasound B-scan imaging, SWIPE offers the potential advantages of image formation without beam focusing or steering from a single transducer element, lateral resolution independent of aperture size, and the potential to achieve relatively high lateral resolution with low frequency ultrasound. Here a Fourier series description of the phase modulated echo signal is developed, demonstrating that echo harmonics at multiples of the shear wave frequency reveal target k-space data at identical multiples of the shear wavenumber. Modulation transfer functions of SWIPE imaging systems are calculated for maximum shear wave acceleration and maximum shear constraints, and compared with a conventionally focused aperture. The relative signal-to-noise ratio of the SWIPE method versus a conventionally focused aperture is found through these calculations. Reconstructions of wire targets in a gelatin phantom using 1 and 3.5 MHz ultrasound and a cylindrical shear wave source are presented, generated from the fundamental and second harmonic of the shear wave modulation frequency, demonstrating weak dependence of lateral resolution with ultrasound frequency. PMID:24815265

  2. 2D magnetic nanoparticle imaging using magnetization response second harmonic

    NASA Astrophysics Data System (ADS)

    Tanaka, Saburo; Murata, Hayaki; Oishi, Tomoya; Suzuki, Toshifumi; Zhang, Yi

    2015-06-01

    A detection method and an imaging technique for magnetic nanoparticles (MNPs) have been investigated. In MNP detection and in magnetic particle imaging (MPI), the most commonly employed method is the detection of the odd harmonics of the magnetization response. We examined the advantage of using the second harmonic response when applying an AC magnetic modulation field and a DC bias field. If the magnetization response is detected by a Cu-wound-coil detection system, the output voltage from the coil is proportional to the change in the flux, dϕ/dt. Thus, the dependence of the derivative of the magnetization, M, on an AC magnetic modulation field and a DC bias field were calculated and investigated. The calculations were in good agreement with the experimental results. We demonstrated that the use of the second harmonic response for the detection of MNPs has an advantage compared with the usage of the third harmonic response, when the Cu-wound-coil detection system is employed and the amplitude of the ratio of the AC modulation field and a knee field Hac/Hk is less than 2. We also constructed a 2D MPI scanner using a pair of permanent ring magnets with a bore of ϕ80 mm separated by 90 mm. The magnets generated a gradient of Gz=3.17 T/m transverse to the imaging bore and Gx=1.33 T/m along the longitudinal axis. An original concentrated 10 μl Resovist solution in a ϕ2×3 mm2 vessel was used as a sample, and it was imaged by the scanner. As a result, a 2D contour map image could be successfully generated using the method with a lock-in amplifier.

  3. Spatial harmonic imaging of X-ray scattering--initial results.

    PubMed

    Wen, Han; Bennett, Eric E; Hegedus, Monica M; Carroll, Stefanie C

    2008-08-01

    Coherent X-ray scattering is related to the electron density distribution by a Fourier transform, and therefore a window into the microscopic structures of biological samples. Current techniques of scattering rely on small-angle measurements from highly collimated X-ray beams produced from synchrotron light sources. Imaging of the distribution of scattering provides a new contrast mechanism which is different from absorption radiography, but is a lengthy process of raster or line scans of the beam over the object. Here, we describe an imaging technique in the spatial frequency domain capable of acquiring both the scattering and absorption distributions in a single exposure. We present first results obtained with conventional X-ray equipment. This method interposes a grid between the X-ray source and the imaged object, so that the grid-modulated image contains a primary image and a grid harmonic image. The ratio between the harmonic and primary images is shown to be a pure scattering image. It is the auto-correlation of the electron density distribution at a specific distance. We tested a number of samples at 60-200 nm autocorrelation distance, and found the scattering images to be distinct from the absorption images and reveal new features. This technique is simple to implement, and should help broaden the imaging applications of X-ray scattering. PMID:18672418

  4. Automated cardiac sarcomere analysis from second harmonic generation images

    NASA Astrophysics Data System (ADS)

    Garcia-Canadilla, Patricia; Gonzalez-Tendero, Anna; Iruretagoyena, Igor; Crispi, Fatima; Torre, Iratxe; Amat-Roldan, Ivan; Bijnens, Bart H.; Gratacos, Eduard

    2014-05-01

    Automatic quantification of cardiac muscle properties in tissue sections might provide important information related to different types of diseases. Second harmonic generation (SHG) imaging provides a stain-free microscopy approach to image cardiac fibers that, combined with our methodology of the automated measurement of the ultrastructure of muscle fibers, computes a reliable set of quantitative image features (sarcomere length, A-band length, thick-thin interaction length, and fiber orientation). We evaluated the performance of our methodology in computer-generated muscle fibers modeling some artifacts that are present during the image acquisition. Then, we also evaluated it by comparing it to manual measurements in SHG images from cardiac tissue of fetal and adult rabbits. The results showed a good performance of our methodology at high signal-to-noise ratio of 20 dB. We conclude that our automated measurements enable reliable characterization of cardiac fiber tissues to systematically study cardiac tissue in a wide range of conditions.

  5. Image enhancement by nonlinear extrapolation in frequency space

    NASA Astrophysics Data System (ADS)

    Greenspan, Hayit; Anderson, Charles H.

    1994-03-01

    A procedure for creating images with higher resolution than the sampling rate would allow is described. The enhancement algorithm augments the frequency content of the image using shape-invariant properties of edges across scale by using a non-linearity that generates phase- coherent higher harmonics. The procedure utilizes the Laplacian pyramid image representation. Results are presented depicting the power-spectra augmentation and the visual enhancement of several images. Simplicity of computations and ease of implementation allow for real-time applications such as high-definition television.

  6. Single pulse frequency compounding protocol for superharmonic imaging

    NASA Astrophysics Data System (ADS)

    Danilouchkine, M. G.; van Neer, P. L. M. J.; Verweij, M. D.; Matte, G. M.; Vletter, W. B.; van der Steen, A. F. W.; de Jong, N.

    2013-07-01

    Second harmonic imaging is currently accepted as the standard in commercial echographic systems. A new imaging technique, coined as superharmonic imaging (SHI), combines the third till the fifth harmonics, arising during nonlinear sound propagation. It could further enhance the resolution and quality of echographic images. To meet the bandwidth requirement for SHI a dedicated phased array has been developed: a low frequency subarray, intended for transmission, interleaved with a high frequency subarray, used in reception. As the bandwidth of the elements is limited, the spectral gaps in between the harmonics cause multiple (ghost) reflection artifacts. A dual-pulse frequency compounding method aims at suppressing those artifacts at a price of a reduced frame rate. In this study we explore a possibility of performing frequency compounding within a single transmission. The traditional frequency compounding method suppresses the ripples by consecutively emitting two short Gaussian bursts with a slightly different center frequency. In the newly proposed method, the transmit aperture is divided into two parts: the first half is used to send a pulse at the lower center frequency, while the other half simultaneously transmits at a slightly higher center frequency. The suitability of the protocol for medical imaging applications in terms of the steering capabilities was performed in a simulation study with INCS and the hydrophone measurements. Moreover, an experimental study was carried out to find the optimal parameters for the clinical imaging protocol. The latter was subsequently used to obtain the images of a tissue mimicking phantom containing strongly reflecting wires. Additionally, the images of a human heart in the parasternal projection were acquired. The scanning aperture with the developed protocol amounts to approximately 90°, which is sufficient to capture the cardiac structures in the standard anatomical projections. The theoretically estimated and

  7. Examining the impact of harmonic correlation on vibrational frequencies calculated in localized coordinates

    SciTech Connect

    Hanson-Heine, Magnus W. D.

    2015-10-28

    Carefully choosing a set of optimized coordinates for performing vibrational frequency calculations can significantly reduce the anharmonic correlation energy from the self-consistent field treatment of molecular vibrations. However, moving away from normal coordinates also introduces an additional source of correlation energy arising from mode-coupling at the harmonic level. The impact of this new component of the vibrational energy is examined for a range of molecules, and a method is proposed for correcting the resulting self-consistent field frequencies by adding the full coupling energy from connected pairs of harmonic and pseudoharmonic modes, termed vibrational self-consistent field (harmonic correlation). This approach is found to lift the vibrational degeneracies arising from coordinate optimization and provides better agreement with experimental and benchmark frequencies than uncorrected vibrational self-consistent field theory without relying on traditional correlated methods.

  8. Frequency locking of a field-widened Michelson interferometer based on optimal multi-harmonics heterodyning.

    PubMed

    Cheng, Zhongtao; Liu, Dong; Zhou, Yudi; Yang, Yongying; Luo, Jing; Zhang, Yupeng; Shen, Yibing; Liu, Chong; Bai, Jian; Wang, Kaiwei; Su, Lin; Yang, Liming

    2016-09-01

    A general resonant frequency locking scheme for a field-widened Michelson interferometer (FWMI), which is intended as a spectral discriminator in a high-spectral-resolution lidar, is proposed based on optimal multi-harmonics heterodyning. By transferring the energy of a reference laser to multi-harmonics of different orders generated by optimal electro-optic phase modulation, the heterodyne signal of these multi-harmonics through the FWMI can reveal the resonant frequency drift of the interferometer very sensitively within a large frequency range. This approach can overcome the locking difficulty induced by the low finesse of the FWMI, thus contributing to excellent locking accuracy and lock acquisition range without any constraint on the interferometer itself. The theoretical and experimental results are presented to verify the performance of this scheme. PMID:27607936

  9. Artificial Ionization and UHF Radar Response Associated with HF Frequencies near Electron Gyro-Harmonics (Invited)

    NASA Astrophysics Data System (ADS)

    Watkins, B. J.; Fallen, C. T.; Secan, J. A.

    2013-12-01

    We present new results from O-mode ionospheric heating experiments at the HAARP facility in Alaska to demonstrate that the magnitude of artificial ionization production is critically dependent on the choice of HF frequency near gyro-harmonics. For O-mode heating in the lower F-region ionosphere, typically about 200 km altitude, artificial ionization enhancements are observed in the lower ionosphere (about 150 - 220 km) and also in the topside ionosphere above about 500 km. Lower ionosphere density enhancements are inferred from HF-enhanced ion and plasma-line signals observed with UHF radar. Upper ionospheric density enhancements have been observed with TEC (total electron content) experiments by monitoring satellite radio beacons where signal paths traverse the HF-modified ionosphere. Both density enhancements and corresponding upward plasma fluxes have also been observed in the upper ionosphere via in-situ satellite observations. The data presented focus mainly on observations near the third and fourth gyro-harmonics. The specific values of the height-dependent gyro-harmonics have been computed from a magnetic model of the field line through the HF heated volume. Experiments with several closely spaced HF frequencies around the gyro-harmonic frequency region show that the magnitude of the lower-ionosphere artificial ionization production maximizes for HF frequencies about 1.0 - 1.5 MHz above the gyro-harmonic frequency. The response is progressively larger as the HF frequency is increased in the frequency region near the gyro-harmonics. For HF frequencies that are initially greater than the gyro-harmonic value the UHF radar scattering cross-section is relatively small, and non-existent or very weak signals are observed; as the signal returns drop in altitude due to density enhancements the HF interaction region passes through lower altitudes where the HF frequency is less than the gyro-harmonic value, for these conditions the radar scattering cross-section is

  10. Electron cyclotron harmonic resonances in high-frequency heating of the ionosphere

    SciTech Connect

    Kuo, Spencer P.

    2013-09-15

    Electron acceleration by upper hybrid waves under cyclotron harmonic resonance interaction is studied. Theory is formulated; the analytical solutions in the second and fourth harmonic cyclotron resonance cases are obtained, and in the third harmonic case, a first order differential equation governing the evolution of the electron energy is derived. The theory is applied for explaining the generation of artificial ionization layers observed in high-frequency (HF) ionospheric heating experiments. The upper hybrid waves are assumed to be excited parametrically by the O-mode HF heating wave. As the decay mode is the lower hybrid wave, the excited upper hybrid waves have wavelengths ranging from 0.25 to 0.5 m, which are short enough to effectively incorporate the finite Larmour radius effect for the harmonic cyclotron resonance interactions as well as have a frequency bandwidth of about 20 kHz, which provides an altitude region of about 10 km for continuous harmonic cyclotron resonance interaction between electrons and descending waves in the slightly inhomogeneous geomagnetic field. The numerical results on electron acceleration show that electron fluxes with energies larger than 14 eV are generated in the three harmonic cases. These energetic electrons cause impact ionizations, which are descending to form artificial ionization layers at the bottom of the ionospheric F region.

  11. Frequency dependence of quantum path interference in non-collinear high-order harmonic generation

    NASA Astrophysics Data System (ADS)

    Shi-Yang, Zhong; Xin-Kui, He; Hao, Teng; Peng, Ye; Li-Feng, Wang; Peng, He; Zhi-Yi, Wei

    2016-02-01

    High-order harmonic generation (HHG) driven by two non-collinear beams including a fundamental and its weak second harmonic is numerically studied. The interference of harmonics from adjacent electron quantum paths is found to be dependent on the relative delay of the driving pulse, and the dependences are different for different harmonic orders. This frequency dependence of the interference is attributed to the spatial frequency chirp in the HHG beam resulting from the harmonic dipole phase, which in turn provides a potential way to gain an insight into the generation of high-order harmonics. As an example, the intensity dependent dipole phase coefficient α is retrieved from the interference fringe. Project supported by the National Key Basic Research Program of China (Grant Nos. 2013CB922401 and 2013CB922402), the National Key Scientific Instrument and Equipment Development Projects, China (Grant No. 2012YQ12004704), the National Natural Science Foundation of China (Grant No. 11374356), and the International Joint Research Program of National Natural Science Foundation of China (Grant No. 61210017).

  12. Localized, Non-Harmonic Active Flap Motions for Low Frequency In-Plane Rotor Noise Reduction

    NASA Technical Reports Server (NTRS)

    Sim, Ben W.; Potsdam, Mark; Kitaplioglu, Cahit; LeMasurier, Philip; Lorber, Peter; Andrews, Joseph

    2012-01-01

    A first-of-its-kind demonstration of the use of localized, non-harmonic active flap motions, for suppressing low frequency, in-plane rotor noise, is reported in this paper. Operational feasibility is verified via testing of the full-scale AATD/Sikorsky/UTRC active flap demonstration rotor in the NFAC's 40- by 80-Foot anechoic wind tunnel. Effectiveness of using localized, non-harmonic active flap motions are compared to conventional four-per-rev harmonic flap motions, and also active flap motions derived from closed-loop acoustics implementations. All three approaches resulted in approximately the same noise reductions over an in-plane three-by-three microphone array installed forward and near in-plane of the rotor in the nearfield. It is also reported that using an active flap in this localized, non-harmonic manner, resulted in no more that 2% rotor performance penalty, but had the tendency to incur higher hub vibration levels.

  13. Interpreting Second-Harmonic Generation Images of Collagen I Fibrils

    PubMed Central

    Williams, Rebecca M.; Zipfel, Warren R.; Webb, Watt W.

    2005-01-01

    Fibrillar collagen, being highly noncentrosymmetric, possesses a tremendous nonlinear susceptibility. As a result, second-harmonic generation (SHG) microscopy of collagen produces extremely bright and robust signals, providing an invaluable tool for imaging tissue structure with submicron resolution. Here we discuss fundamental principles governing SHG phase matching with the tightly focusing optics used in microscopy. Their application to collagen imaging yields several biophysical features characteristic of native collagen structure: SHG radiates from the shell of a collagen fibril, rather than from its bulk. This SHG shell may correspond to the supporting element of the fibril. Physiologically relevant changes in solution ionic strength alter the ratio of forward-to-backward propagating SHG, implying a resulting change in the SHG shell thickness. Fibrillogenesis can be resolved in immature tissue by directly imaging backward-propagating SHG. Such findings are crucial to the design and development of forthcoming diagnostic and research tools. PMID:15533922

  14. Research of second harmonic generation images based on texture analysis

    NASA Astrophysics Data System (ADS)

    Liu, Yao; Li, Yan; Gong, Haiming; Zhu, Xiaoqin; Huang, Zufang; Chen, Guannan

    2014-09-01

    Texture analysis plays a crucial role in identifying objects or regions of interest in an image. It has been applied to a variety of medical image processing, ranging from the detection of disease and the segmentation of specific anatomical structures, to differentiation between healthy and pathological tissues. Second harmonic generation (SHG) microscopy as a potential noninvasive tool for imaging biological tissues has been widely used in medicine, with reduced phototoxicity and photobleaching. In this paper, we clarified the principles of texture analysis including statistical, transform, structural and model-based methods and gave examples of its applications, reviewing studies of the technique. Moreover, we tried to apply texture analysis to the SHG images for the differentiation of human skin scar tissues. Texture analysis method based on local binary pattern (LBP) and wavelet transform was used to extract texture features of SHG images from collagen in normal and abnormal scars, and then the scar SHG images were classified into normal or abnormal ones. Compared with other texture analysis methods with respect to the receiver operating characteristic analysis, LBP combined with wavelet transform was demonstrated to achieve higher accuracy. It can provide a new way for clinical diagnosis of scar types. At last, future development of texture analysis in SHG images were discussed.

  15. The radio-frequency fluctuation effect on the floating harmonic method

    NASA Astrophysics Data System (ADS)

    Lee, Jaewon; Kim, Kyung-Hyun; Kim, Dong-Hwan; Chung, Chin-Wook

    2016-08-01

    The radio-frequency (RF) plasma diagnostics with an electrical probe facing a challenge, because the RF fluctuation oscillates the plasma potential and distorts the current-voltage (I-V) curve. As Langmuir probe is widely used in plasma diagnostics, many researchers have been studying the effect of RF fluctuation on probe and compensation methods. On the other hand, there have not been enough studies on the fluctuation effect on the floating harmonic method. Therefore, we investigated the impact of RF fluctuation on the floating harmonic method theoretically and experimentally. When the electrons are in ideal Maxwellian distribution, the floating potential is negatively shifted by the RF fluctuation, but the fluctuation does not distort I-V curve around the floating potential. However, in practical plasmas, the I-V curve and their harmonic components are distorted. This RF fluctuation effect becomes more significant in a low density plasma with a high impedance sheath. The second harmonic current decreases with the RF fluctuation while the first harmonic current is merely affected. Therefore, the electron temperatures measured with the floating harmonic method under low density plasma with uncompensated probe are overestimated than the results obtained with the compensated probe.

  16. Value of tissue harmonic imaging (THI) and contrast harmonic imaging (CHI) in detection and characterisation of breast tumours

    PubMed Central

    Jung, E. M.; Jungius, K.-P.; Ertan, K.

    2006-01-01

    The purpose of this study was to investigate the extent to which tissue harmonic imaging (THI), speckle reduction imaging (SRI), spatial compounding (SC) and contrast can improve detection and differentiation of breast tumours. We examined 38 patients (14 benign, 24 malignant tumours) with different combinations of THI, SRI and SC. The effect on delineation, margin, tissue differentiation and posttumoral phenomena was evaluated with a three-point score. Additionally, 1oo not palpable tumours (diameters: 4–15 mm) were examined by contrast harmonic imaging (CHI) with power Doppler. After bolus injection (0.5 ml Optison), vascularisation and enhancement were observed for 20 min. The best combination for detection of margin, infiltration, echo pattern and posterior lesion boundary was the combination of SRI level 2 with SC low. THI was helpful for lesions OF more than 1 cm depth. In native Power Doppler, vessels were found in 54 of 100 lesions. Within 5 min after contrast medium (CM) injection, marginal and penetrating vessels increased in benign and malignant tumours and central vessels mostly in carcinomas (p<0.05). A diffuse CM accumulation was observed up to 20 min after injection in malignant tumours only (p<0.05). THI, SRI and SC improved delineation and tissue differentiation. Second-generation contrast agent allowed detection of tumour vascularisation with prolonged enhancement. PMID:16823568

  17. Imaging morphodynamics of human blood cells in vivo with video-rate third harmonic generation microscopy

    PubMed Central

    Chen, Chien-Kuo; Liu, Tzu-Ming

    2012-01-01

    With a video-rate third harmonic generation (THG) microscopy system, we imaged the micro-circulation beneath the human skin without labeling. Not only the speed of circulation but also the morpho-hydrodynamics of blood cells can be analyzed. Lacking of nuclei, red blood cells (RBCs) shows typical parachute-like and hollow-core morphology under THG microscopy. Quite different from RBCs, every now and then, round and granule rich blood cells with strong THG contrast appear in circulation. The corresponding volume densities in blood, evaluated from their frequencies of appearance and the velocity of circulation, fall within the physiological range of human white blood cell counts. PMID:23162724

  18. Imaging morphodynamics of human blood cells in vivo with video-rate third harmonic generation microscopy.

    PubMed

    Chen, Chien-Kuo; Liu, Tzu-Ming

    2012-11-01

    With a video-rate third harmonic generation (THG) microscopy system, we imaged the micro-circulation beneath the human skin without labeling. Not only the speed of circulation but also the morpho-hydrodynamics of blood cells can be analyzed. Lacking of nuclei, red blood cells (RBCs) shows typical parachute-like and hollow-core morphology under THG microscopy. Quite different from RBCs, every now and then, round and granule rich blood cells with strong THG contrast appear in circulation. The corresponding volume densities in blood, evaluated from their frequencies of appearance and the velocity of circulation, fall within the physiological range of human white blood cell counts. PMID:23162724

  19. Dual-frequency piezoelectric transducers for contrast enhanced ultrasound imaging.

    PubMed

    Martin, K Heath; Lindsey, Brooks D; Ma, Jianguo; Lee, Mike; Li, Sibo; Foster, F Stuart; Jiang, Xiaoning; Dayton, Paul A

    2014-01-01

    For many years, ultrasound has provided clinicians with an affordable and effective imaging tool for applications ranging from cardiology to obstetrics. Development of microbubble contrast agents over the past several decades has enabled ultrasound to distinguish between blood flow and surrounding tissue. Current clinical practices using microbubble contrast agents rely heavily on user training to evaluate degree of localized perfusion. Advances in separating the signals produced from contrast agents versus surrounding tissue backscatter provide unique opportunities for specialized sensors designed to image microbubbles with higher signal to noise and resolution than previously possible. In this review article, we describe the background principles and recent developments of ultrasound transducer technology for receiving signals produced by contrast agents while rejecting signals arising from soft tissue. This approach relies on transmitting at a low-frequency and receiving microbubble harmonic signals at frequencies many times higher than the transmitted frequency. Design and fabrication of dual-frequency transducers and the extension of recent developments in transducer technology for dual-frequency harmonic imaging are discussed. PMID:25375755

  20. Dual-Frequency Piezoelectric Transducers for Contrast Enhanced Ultrasound Imaging

    PubMed Central

    Martin, K. Heath; Lindsey, Brooks D.; Ma, Jianguo; Lee, Mike; Li, Sibo; Foster, F. Stuart; Jiang, Xiaoning; Dayton, Paul A.

    2014-01-01

    For many years, ultrasound has provided clinicians with an affordable and effective imaging tool for applications ranging from cardiology to obstetrics. Development of microbubble contrast agents over the past several decades has enabled ultrasound to distinguish between blood flow and surrounding tissue. Current clinical practices using microbubble contrast agents rely heavily on user training to evaluate degree of localized perfusion. Advances in separating the signals produced from contrast agents versus surrounding tissue backscatter provide unique opportunities for specialized sensors designed to image microbubbles with higher signal to noise and resolution than previously possible. In this review article, we describe the background principles and recent developments of ultrasound transducer technology for receiving signals produced by contrast agents while rejecting signals arising from soft tissue. This approach relies on transmitting at a low-frequency and receiving microbubble harmonic signals at frequencies many times higher than the transmitted frequency. Design and fabrication of dual-frequency transducers and the extension of recent developments in transducer technology for dual-frequency harmonic imaging are discussed. PMID:25375755

  1. Improving harmonic vibrational frequencies calculations in density functional theory

    NASA Astrophysics Data System (ADS)

    Stratmann, R. Eric; Burant, John C.; Scuseria, Gustavo E.; Frisch, Michael J.

    1997-06-01

    Using a previously introduced weight scheme, microbatching, and grid compression [R. E. Stratmann, G. E. Scuseria and M. J. Frisch, Chem. Phys. Lett. 257, 213 (1996)], we significantly speed up the numerical integration of the exchange-correlation contribution to the Coupled-Perturbed Kohn-Sham equations. In addition, we find that the nature of the integrand is such that it is possible to employ substantially fewer grid points in the quadrature and to use the Gaussian very Fast Multipole Method (GvFMM) with very short multipole expansions for the Coulomb contribution, with negligible loss in accuracy. As a representative example, the computational demand for the exchange-correlation portion of a coronene (C24H12) frequency calculation with a 3-21G basis is reduced by more than one order of magnitude. The overall speed up achieved in this calculation is between a factor of 4 to 6, depending on the specific functional. We also present sample calculations using polarized bases, gradient-corrected functionals, and on even larger systems (C54H18 and C96H24), to illustrate the various effects and improvements that we have accomplished.

  2. Light transport in biological tissue using three-dimensional frequency-domain simplified spherical harmonics equations

    NASA Astrophysics Data System (ADS)

    Chu, Michael; Vishwanath, Karthik; Klose, Alexander D.; Dehghani, Hamid

    2009-04-01

    The accuracy of the commonly used diffusion approximation as used in diffuse optical tomography is known to be limited in cases involving strong absorption and in these situations a higher ordered approximation is necessary. In this study, a light transport model has been developed based upon the three-dimensional frequency-domain simplified spherical harmonics (SPN) approximation for orders up to N = 7. The SPN data are tested against a semi-infinite multi-layered Monte Carlo model. It has been shown that the SPN approximation for higher orders (N >1) provides an increase in accuracy over the diffusion equation specifically near sources and at boundaries of regions with increased optical absorption. It is demonstrated that the error of fluence calculated near the sources between the diffusion approximation and the SPN model (N = 7) can be as large as 60%, therefore limiting the use of the diffusion approximation for small animal imaging and in situations where optical changes near sources are critical for tomographic reconstructions.

  3. Frequency modulation of high-order harmonic generation in an orthogonally polarized two-color laser field.

    PubMed

    Li, Guicun; Zheng, Yinghui; Ge, Xiaochun; Zeng, Zhinan; Li, Ruxin

    2016-08-01

    We have experimentally investigated the frequency modulation of high-order harmonics in an orthogonally polarized two-color laser field consisting of a mid-infrared 1800nm fundamental pulse and its second harmonic pulse. It is demonstrated that the high harmonic spectra can be fine-tuned as we slightly change the relative delay of the two-color laser pulses. By analyzing the relative frequency shift of each harmonic at different two-color delays, the nonadiabatic spectral shift induced by the rapid variation of the intensity-dependent intrinsic dipole phase can be distinguished from the blueshift induced by the change of the refractive index during self-phase modulation (SPM). Our comprehensive analysis shows that the frequency modulation pattern is a reflection of the average emission time of high-order harmonic generation (HHG), thus offering a simple method to fine-tune the spectra of the harmonics on a sub-cycle time scale. PMID:27505831

  4. Temperature dependence of acoustic harmonics generated by nonlinear ultrasound wave propagation in water at various frequencies.

    PubMed

    Maraghechi, Borna; Hasani, Mojtaba H; Kolios, Michael C; Tavakkoli, Jahan

    2016-05-01

    Ultrasound-based thermometry requires a temperature-sensitive acoustic parameter that can be used to estimate the temperature by tracking changes in that parameter during heating. The objective of this study is to investigate the temperature dependence of acoustic harmonics generated by nonlinear ultrasound wave propagation in water at various pulse transmit frequencies from 1 to 20 MHz. Simulations were conducted using an expanded form of the Khokhlov-Zabolotskaya-Kuznetsov nonlinear acoustic wave propagation model in which temperature dependence of the medium parameters was included. Measurements were performed using single-element transducers at two different transmit frequencies of 3.3 and 13 MHz which are within the range of frequencies simulated. The acoustic pressure signals were measured by a calibrated needle hydrophone along the axes of the transducers. The water temperature was uniformly increased from 26 °C to 46 °C in increments of 5 °C. The results show that the temperature dependence of the harmonic generation is different at various frequencies which is due to the interplay between the mechanisms of absorption, nonlinearity, and focusing gain. At the transmit frequencies of 1 and 3.3 MHz, the harmonic amplitudes decrease with increasing the temperature, while the opposite temperature dependence is observed at 13 and 20 MHz. PMID:27250143

  5. Two-Dimensional Frequency Resolved Optomolecular Gating of High-Order Harmonic Generation.

    PubMed

    Ferré, A; Soifer, H; Pedatzur, O; Bourassin-Bouchet, C; Bruner, B D; Canonge, R; Catoire, F; Descamps, D; Fabre, B; Mével, E; Petit, S; Dudovich, N; Mairesse, Y

    2016-02-01

    Probing electronic wave functions of polyatomic molecules is one of the major challenges in high-harmonic spectroscopy. The extremely nonlinear nature of the laser-molecule interaction couples the multiple degrees of freedom of the probed system. We combine two-dimensional control of the electron trajectories and vibrational control of the molecules to disentangle the two main steps in high-harmonic generation-ionization and recombination. We introduce a new measurement scheme, frequency-resolved optomolecular gating, which resolves the temporal amplitude and phase of the harmonic emission from excited molecules. Focusing on the study of vibrational motion in N_{2}O_{4}, we show that such advanced schemes provide a unique insight into the structural and dynamical properties of the underlying mechanism. PMID:26894708

  6. Cochlear implant melody recognition as a function of melody frequency range, harmonicity, and number of electrodes

    PubMed Central

    Singh, Sonya; Kong, Ying-Yee; Zeng, Fan-Gang

    2009-01-01

    Objective The primary goal of the present study was to determine how cochlear implant melody recognition was affected by the frequency range of the melodies, the harmonicity of these melodies, and the number of activated electrodes. The secondary goal was to investigate whether melody recognition and speech recognition were differentially affected by the limitations imposed by cochlear implant processing. Design Four experiments were conducted. In the first experiment, eleven cochlear implant users used their clinical processors to recognize melodies of complex harmonic tones with their fundamental frequencies being in the low (104-262 Hz), middle (207-523 Hz), and high (414-1046 Hz) ranges. In the second experiment, melody recognition with pure tones was compared to melody recognition with complex harmonic tones in 4 subjects. In the third experiment, melody recognition was measured as a function of the number of electrodes in 5 subjects. In the fourth experiment, vowel and consonant recognition were measured as a function of the number of electrodes in the same 5 subjects who participated in the third experiment. Results Frequency range significantly affected cochlear implant melody recognition with higher frequency ranges producing better performance. Pure tones produced significantly better performance than complex harmonic tones. Increasing the number of activated electrodes did not affect performance with low- and middle-frequency melodies, but produced better performance with high-frequency melodies. Large individual variability was observed for melody recognition but its source seemed to be different from the source of the large variability observed in speech recognition. Conclusion Contemporary cochlear implants do not adequately encode either temporal pitch or place pitch cues. Melody recognition and speech recognition require different signal processing strategies in future cochlear implants. PMID:19194298

  7. Imaging articular cartilage using second harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Mansfield, Jessica C.; Winlove, C. Peter; Knapp, Karen; Matcher, Stephen J.

    2006-02-01

    Sub cellular resolution images of equine articular cartilage have been obtained using both second harmonic generation microscopy (SHGM) and two-photon fluorescence microscopy (TPFM). The SHGM images clearly map the distribution of the collagen II fibers within the extracellular matrix while the TPFM images show the distribution of endogenous two-photon fluorophores in both the cells and the extracellular matrix, highlighting especially the pericellular matrix and bright 2-3μm diameter features within the cells. To investigate the source of TPF in the extracellular matrix experiments have been carried out to see if it may originate from the proteoglycans. Pure solutions of the following proteoglycans hyaluronan, chondroitin sulfate and aggrecan have been imaged, only the aggrecan produced any TPF and here the intensity was not great enough to account for the TPF in the extracellular matrix. Also cartilage samples were subjected to a process to remove proteoglycans and cellular components. After this process the TPF from the samples had decreased by a factor of two, with respect to the SHG intensity.

  8. Evading surface and detector frequency noise in harmonic oscillator measurements of force gradients

    PubMed Central

    Moore, Eric W.; Lee, SangGap; Hickman, Steven A.; Harrell, Lee E.; Marohn, John A.

    2010-01-01

    We introduce and demonstrate a method of measuring small force gradients acting on a harmonic oscillator in which the force-gradient signal of interest is used to parametrically up-convert a forced oscillation below resonance into an amplitude signal at the oscillator’s resonance frequency. The approach, which we demonstrate in a mechanically detected electron spin resonance experiment, allows the force-gradient signal to evade detector frequency noise by converting a slowly modulated frequency signal into an amplitude signal. PMID:20733934

  9. Generation of harmonic frequencies and their effects in present day ICRF systems

    NASA Astrophysics Data System (ADS)

    Durodié, F.; Vervier, M.

    1999-09-01

    The whole TEXTOR-94 ICRF system [1,2] circuit has been analyzed, partially modelled and studied at the fundamental operating frequency and, more important, at harmonic frequencies thereof. It has been found that without appropriate measures, present day systems and especially the TEXTOR ICRF system are prone to spurious generation of power at the second harmonic frequency. This leads either to erroneous activation of the reflected power safety protection or to highly increased (in some cases doubled) voltages in the transmission lines and antennas. As the voltage standing waves at the second harmonic displace the total voltage maxima in the transmission lines and antennas, this also explains why arcs are not always found at the expected voltage maxima for the fundamental frequency. The model is also able to give additional explanations why the ASDEX-Upgrade ICRF system has dramatically improved its power handling capabilities after the introduction of the ``3dB coupler reflection compensation scheme'' [3,4]. The output of one of the TEXTOR transmitters has been fitted with a quarter wave length shorted stub which has no effect on the operation at the fundamental frequency but which effectively shorts out the second harmonic. It has to be noted that eg. the Tore Supra RDL [5] antennas are fitted with such a stub in the feeding transmission line whose function is to enter cooling water into the transmission line system. Hence, this could be the explanation of the apparent higher than average power handling capabilities of the TS antenna system. Experimental results clearly indicate a much improved operational power handling capability on plasma and an increased voltage stand-off when conditioning this antenna pair on vacuum. Limits have yet to be explored but already remarkable is the fact that since the installation of the quarter wavelength stub the reflected power safety system has been activated only once and furthermore so in poorly matched conditions.

  10. Nonlinear synthetic aperture radar imaging using a harmonic radar

    NASA Astrophysics Data System (ADS)

    Gallagher, Kyle A.; Mazzaro, Gregory J.; Ranney, Kenneth I.; Nguyen, Lam H.; Martone, Anthony F.; Sherbondy, Kelly D.; Narayanan, Ram M.

    2015-05-01

    This paper presents synthetic aperture radar (SAR) images of linear and nonlinear targets. Data are collected using a linear/nonlinear step frequency radar. We show that it is indeed possible to produce SAR images using a nonlinear radar. Furthermore, it is shown that the nonlinear radar is able to reduce linear clutter by at least 80 dB compared to a linear radar. The nonlinear SAR images also show the system's ability to detect small electronic devices in the presence of large linear clutter. The system presented here has the ability to completely ignore a 20-inch trihedral corner reflector while detecting a RF mixer with a dipole antenna attached.

  11. Second harmonic generation imaging in tissue engineering and cartilage pathologies

    NASA Astrophysics Data System (ADS)

    Lilledahl, Magnus; Olderøy, Magnus; Finnøy, Andreas; Olstad, Kristin; Brinchman, Jan E.

    2015-03-01

    The second harmonic generation from collagen is highly sensitive to what extent collagen molecules are ordered into fibrils as the SHG signal is approximately proportional to the square of the fibril thickness. This can be problematic when interpreting SHG images as thick fibers are much brighter than thinner fibers such that quantification of the amount of collagen present is difficult. On the other hand SHG is therefore also a very sensitive probe to determine whether collagen have assembled into fibrils or are still dissolved as individual collagen molecules. This information is not available from standard histology or immunohistochemical techniques. The degree for fibrillation is an essential component for proper tissue function. We will present the usefulness of SHG imaging in tissue engineering of cartilage as well as cartilage related pathologies. When engineering cartilage it is essential to have the appropriate culturing conditions which cause the collagen molecules to assemble into fibrils. By employing SHG imaging we have studied how cell seeding densities affect the fibrillation of collagen molecules. Furthermore we have used SHG to study pathologies in developing cartilage in a porcine model. In both cases SHG reveals information which is not visible in conventional histology or immunohistochemistry

  12. Theoretical Characterization of Visual Signatures and Calculation of Approximate Global Harmonic Frequency Scaling Factors

    NASA Astrophysics Data System (ADS)

    Kashinski, D. O.; Nelson, R. G.; Chase, G. M.; di Nallo, O. E.; Byrd, E. F. C.

    2016-05-01

    We are investigating the accuracy of theoretical models used to predict the visible, ultraviolet, and infrared spectra, as well as other properties, of product materials ejected from the muzzle of currently fielded systems. Recent advances in solid propellants has made the management of muzzle signature (flash) a principle issue in weapons development across the calibers. A priori prediction of the electromagnetic spectra of formulations will allow researchers to tailor blends that yield desired signatures and determine spectrographic detection ranges. Quantum chemistry methods at various levels of sophistication have been employed to optimize molecular geometries, compute unscaled harmonic frequencies, and determine the optical spectra of specific gas-phase species. Electronic excitations are being computed using Time Dependent Density Functional Theory (TD-DFT). Calculation of approximate global harmonic frequency scaling factors for specific DFT functionals is also in progress. A full statistical analysis and reliability assessment of computational results is currently underway. Work supported by the ARL, DoD-HPCMP, and USMA.

  13. CW, single-frequency 229nm laser source for Cd-cooling by harmonic conversion

    NASA Astrophysics Data System (ADS)

    Kaneda, Yushi; Yarborough, J. M.; Merzlyak, Yevgeny

    2015-02-01

    More than 200mW of CW 229nm for Cd atom cooling application was generated by the 4th harmonic of a single frequency optically pumped semiconductor laser using a 10-mm long, Brewster-cut BBO crystal in an external cavity. With 650mW of 458nm input, 216mW of 229nm power was observed. Conversion efficiency from 458nm to 229nm was more than 33%.

  14. Frequency-resolved optical-gating measurements of ultrashort pulses using surface third-harmonic generation

    SciTech Connect

    Tsang, T.; Krumbuegel, M.A.; DeLong, K.W.; Fittinghoff, D.N.; Trebino, R.

    1996-09-01

    We demonstrate what is to our knowledge the first frequency-resolved optical gating (FROG) technique to measure ultrashort pulses from an unamplified Ti:sapphire laser oscillator without direction-of-time ambiguity. This technique utilizes surface third-harmonic generation as the nonlinear-optical effect and, surprisingly, is the most sensitive third-order FROG geometry yet. {copyright} {ital 1996 Optical Society of America.}

  15. A mechanism for plasma waves at the harmonics of the plasma frequency foreshock boundary

    NASA Technical Reports Server (NTRS)

    Klimas, A. J.

    1982-01-01

    A bump-on-tail unstable reduced velocity distribution, constructed from data obtained at the upstream boundary of the electron foreshock by the GSFC electron spectrometer experiment on the ISEE-1 satellite, is used as the initial plasma state for a numerical integration of the 1D-Vlasov-Maxwell system of equations. The integration is carried through the growth of the instability, beyond its saturation, and well into the stabilized plasma regime. A power spectrum computed for the electric field of the stabilized plasma is dominated by a narrow peak at the Bohm-Gross frequency of the unstable field mode but also contains significant power at the harmonics of the Bohm-Gross frequency. The harmonic power is in sharp peaks which are split into closely spaced doublets. The fundamental peak at the Bohm-Gross frequency is split into a closely spaced triplet. The mechanism for excitation of the second harmonic is shown to be second order wave-wave coupling.

  16. Second harmonic generation imaging microscopy of cellular structure and function

    NASA Astrophysics Data System (ADS)

    Millard, Andrew C.; Jin, Lei; Loew, Leslie M.

    2005-03-01

    Second harmonic generation (SHG) imaging microscopy is an important emerging technique for biological research, with many advantages over existing one- or two-photon fluorescence techniques. A non-linear phenomenon employing mode-locked Ti:sapphire or fiber-based lasers, SHG results in intrinsic optical sectioning without the need for a confocal aperture. Furthermore, as a second-order process SHG is confined to loci lacking a center of symmetry. Many important structural proteins such as collagen and cellulose show intrinsic SHG, thus providing access to sub-resolution information on symmetry. However, we are particularly interested here in "resonance-enhanced" SHG from styryl dyes. In general SHG is a combination of a true second-order process and a third-order process dependent on a static electric field, such that SHG from membrane-bound dyes depends on a cell's trans-membrane potential. With simultaneous patch-clamping and non-linear imaging of cells, we have found that SHG is a sensitive probe of trans-membrane potential with sensitivities that are up to four times better than those obtained under optimal conditions using one-photon fluorescence imaging. With the sensitivity of SHG to local electric fields from other sources such as the membrane dipole potential as well as the quadratic dependence of SHG on concentration, we have found that SHG imaging of styryl dyes is also a powerful technique for the investigation of lipid phases and rafts and for the visualization of the dynamics of membrane-vesicle fusion following fertilization of an ovum.

  17. Frequency dependence of optical third-harmonic generation from doped graphene

    NASA Astrophysics Data System (ADS)

    Margulis, Vl. A.; Muryumin, E. E.; Gaiduk, E. A.

    2016-01-01

    In connection with the controversial question about the frequency dependence of the optical third-harmonic generation (THG) from doped graphene, which has recently been discussed in the literature, we develop an analytical theory for the THG susceptibility of doped graphene by using the original Genkin-Mednis nonlinear-conductivity-theory formalism including mixed intra- and interband terms. The theory is free of any nonphysical divergences at zero frequency, and it predicts the main resonant peak in the THG spectrum to be located at the photon energy ħω equal to two thirds of the Fermi energy EF of charge carriers in doped graphene.

  18. Observation of harmonically related solar radio zebra patterns in the 1-4 GHz frequency range

    NASA Astrophysics Data System (ADS)

    Sawant, H. S.; Karlický, M.; Fernandes, F. C. R.; Cecatto, J. R.

    2002-12-01

    A unique case of two zebra patterns related harmonically with ratio of ~ 1:2 was observed by distant radio telescopes at São José dos Campos and Ondřejov Observatories. Accompanied zebras show that the ratio of frequencies of the neighboring zebra lines is in the range of 1.009-1.037. There is a tendency of a decrease of this ratio with decreasing frequency within the specific zebra pattern. Both facts speak in favour of plasma emission models for the zebra pattern fine structure in radio burst continua.

  19. Measurement of optical-beat frequency in a photoconductive terahertz-wave generator using microwave higher harmonics

    NASA Astrophysics Data System (ADS)

    Murasawa, Kengo; Sato, Koki; Hidaka, Takehiko

    2011-05-01

    A new method for measuring optical-beat frequencies in the terahertz (THz) region using microwave higher harmonics is presented. A microwave signal was applied to the antenna gap of a photoconductive (PC) device emitting a continuous electromagnetic wave at about 1 THz by the photomixing technique. The microwave higher harmonics with THz frequencies are generated in the PC device owing to the nonlinearity of the biased photoconductance, which is briefly described in this article. Thirteen nearly periodic peaks in the photocurrent were observed when the microwave was swept from 16 to 20 GHz at a power of -48 dBm. The nearly periodic peaks are generated by the homodyne detection of the optical beat with the microwave higher harmonics when the frequency of the harmonics coincides with the optical-beat frequency. Each peak frequency and its peak width were determined by fitting a Gaussian function, and the order of microwave harmonics was determined using a coarse (i.e., lower resolution) measurement of the optical-beat frequency. By applying the Kalman algorithm to the peak frequencies of the higher harmonics and their standard deviations, the optical-beat frequency near 1 THz was estimated to be 1029.81 GHz with the standard deviation of 0.82 GHz. The proposed method is applicable to a conventional THz-wave generator with a photomixer.

  20. Probing Nuclear Motion by Frequency Modulation of Molecular High-Order Harmonic Generation

    NASA Astrophysics Data System (ADS)

    Bian, Xue-Bin; Bandrauk, André D.

    2014-11-01

    Molecular high-order harmonic generation (MHOHG) in a non-Born-Oppenheimer treatment of H2 + , D2 + , is investigated by numerical simulations of the corresponding time-dependent Schrödinger equations in full dimensions. As opposed to previous studies on amplitude modulation of intracycle dynamics in MHOHG, we demonstrate redshifts as frequency modulation (FM) of intercycle dynamics in MHOHG. The FM is induced by nuclear motion using intense laser pulses. Compared to fixed-nuclei approximations, the intensity of MHOHG is much higher due to the dependence of enhanced ionization on the internuclear distance. The width and symmetry of the spectrum of each harmonic in MHOHG encode rich information on the dissociation process of molecules at the rising and falling parts of the laser pulses, which can be used to retrieve the nuclear dynamics. Isotope effects are studied to confirm the FM mechanism.

  1. Probing nuclear motion by frequency modulation of molecular high-order harmonic generation.

    PubMed

    Bian, Xue-Bin; Bandrauk, André D

    2014-11-01

    Molecular high-order harmonic generation (MHOHG) in a non-Born-Oppenheimer treatment of H(2)(+), D(2)(+), is investigated by numerical simulations of the corresponding time-dependent Schrödinger equations in full dimensions. As opposed to previous studies on amplitude modulation of intracycle dynamics in MHOHG, we demonstrate redshifts as frequency modulation (FM) of intercycle dynamics in MHOHG. The FM is induced by nuclear motion using intense laser pulses. Compared to fixed-nuclei approximations, the intensity of MHOHG is much higher due to the dependence of enhanced ionization on the internuclear distance. The width and symmetry of the spectrum of each harmonic in MHOHG encode rich information on the dissociation process of molecules at the rising and falling parts of the laser pulses, which can be used to retrieve the nuclear dynamics. Isotope effects are studied to confirm the FM mechanism. PMID:25415907

  2. Vector carrier frequency by interfering the kth harmonic of two rulings rotated polarly

    NASA Astrophysics Data System (ADS)

    Meneses-Fabian, Cruz; Kantun-Montiel, Rosaura; Lemus-Alonso, Gildardo-Pablo

    2015-03-01

    This paper presents a method for introducing carrier fringes inclined at any angle into an interferogram. The setup is built on a 4f optical system consisting of two apertures in the input plane and a Ronchi ruling in the Fourier plane. Additionally, a Ronchi ruling rotated on polar direction is placed at each aperture and two passband filters are placed in the Fourier plane for filtering the kth harmonic of their spectra. We demonstrated that the magnitude and direction of the vector carrier frequency depend on the grating period at the input plane, the polar angles, and the kth harmonic, which gives this method the ability to modulate its magnitude only, or its direction only, or both in a wide range. The theoretical model and experimental results are shown in this paper.

  3. Increased efficiency of ion acceleration by using femtosecond laser pulses at higher harmonic frequency

    SciTech Connect

    Psikal, J.; Klimo, O.; Weber, S.; Margarone, D.

    2014-07-15

    The influence of laser frequency on laser-driven ion acceleration is investigated by means of two-dimensional particle-in-cell simulations. When ultrashort intense laser pulse at higher harmonic frequency irradiates a thin solid foil, the target may become re lativistically transparent for significantly lower laser pulse intensity compared with irradiation at fundamental laser frequency. The relativistically induced transparency results in an enhanced heating of hot electrons as well as increased maximum energies of accelerated ions and their numbers. Our simulation results have shown the increase in maximum proton energy and increase in the number of high-energy protons by a factor of 2 after the interaction of an ultrashort laser pulse of maximum intensity 7 × 10{sup 21 }W/cm{sup 2} with a fully ionized plastic foil of realistic density and of optimal thickness between 100 nm and 200 nm when switching from the fundamental frequency to the third harmonics.

  4. Extracting tidal frequencies using multivariate harmonic analysis of sea level height time series

    NASA Astrophysics Data System (ADS)

    Amiri-Simkooei, A. R.; Zaminpardaz, S.; Sharifi, M. A.

    2014-10-01

    This contribution is seen as a first attempt to extract the tidal frequencies using a multivariate spectral analysis method applied to multiple time series of tide-gauge records. The existing methods are either physics-based in which the ephemeris of Moon, Sun and other planets are used, or are observation-based in which univariate analysis methods—Fourier and wavelet for instance—are applied to tidal observations. The existence of many long tide-gauge records around the world allows one to use tidal observations and extract the main tidal constituents for which efficient multivariate methods are to be developed. This contribution applies the multivariate least-squares harmonic estimation (LS-HE) to the tidal time series of the UK tide-gauge stations. The first 413 harmonics of the tidal constituents and their nonlinear components are provided using the multivariate LS-HE. A few observations of the research are highlighted: (1) the multivariate analysis takes information of multiple time series into account in an optimal least- squares sense, and thus the tidal frequencies have higher detection power compared to the univariate analysis. (2) Dominant tidal frequencies range from the long-term signals to the sixth-diurnal species interval. Higher frequencies have negligible effects. (3) The most important tidal constituents (the first 50 frequencies) ordered from their amplitudes range from 212 cm (M2) to 1 cm (OQ2) for the data set considered. There are signals in this list that are not available in the 145 main tidal frequencies of the literature. (4) Tide predictions using different lists of tidal frequencies on five different data sets around the world are compared. The prediction results using the first significant 50 constituents provided promising results on these locations of the world.

  5. Superresolved multiphoton microscopy with spatial frequency-modulated imaging.

    PubMed

    Field, Jeffrey J; Wernsing, Keith A; Domingue, Scott R; Allende Motz, Alyssa M; DeLuca, Keith F; Levi, Dean H; DeLuca, Jennifer G; Young, Michael D; Squier, Jeff A; Bartels, Randy A

    2016-06-14

    Superresolved far-field microscopy has emerged as a powerful tool for investigating the structure of objects with resolution well below the diffraction limit of light. Nearly all superresolution imaging techniques reported to date rely on real energy states of fluorescent molecules to circumvent the diffraction limit, preventing superresolved imaging with contrast mechanisms that occur via virtual energy states, including harmonic generation (HG). We report a superresolution technique based on spatial frequency-modulated imaging (SPIFI) that permits superresolved nonlinear microscopy with any contrast mechanism and with single-pixel detection. We show multimodal superresolved images with two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) from biological and inorganic media. Multiphoton SPIFI (MP-SPIFI) provides spatial resolution up to 2η below the diffraction limit, where η is the highest power of the nonlinear intensity response. MP-SPIFI can be used to provide enhanced resolution in optically thin media and may provide a solution for superresolved imaging deep in scattering media. PMID:27231219

  6. Continuous-Wave Operation of a Frequency-Tunable 460-GHz Second-Harmonic Gyrotron for Enhanced Nuclear Magnetic Resonance.

    PubMed

    Torrezan, Antonio C; Han, Seong-Tae; Mastovsky, Ivan; Shapiro, Michael A; Sirigiri, Jagadishwar R; Temkin, Richard J; Barnes, Alexander B; Griffin, Robert G

    2010-06-01

    The design, operation, and characterization of a continuous-wave (CW) tunable second-harmonic 460-GHz gyrotron are reported. The gyrotron is intended to be used as a submillimeter-wave source for 700-MHz nuclear magnetic resonance experiments with sensitivity enhanced by dynamic nuclear polarization. The gyrotron operates in the whispering-gallery mode TE(11,2) and has generated 16 W of output power with a 13-kV 100-mA electron beam. The start oscillation current measured over a range of magnetic field values is in good agreement with theoretical start currents obtained from linear theory for successive high-order axial modes TE(11,2,q). The minimum start current is 27 mA. Power and frequency tuning measurements as a function of the electron cyclotron frequency have also been carried out. A smooth frequency tuning range of 1 GHz was obtained for the operating second-harmonic mode either by magnetic field tuning or beam voltage tuning. Long-term CW operation was evaluated during an uninterrupted period of 48 h, where the gyrotron output power and frequency were kept stable to within ±0.7% and ±6 ppm, respectively, by a computerized control system. Proper operation of an internal quasi-optical mode converter implemented to transform the operating whispering-gallery mode to a Gaussian-like beam was also verified. Based on the images of the gyrotron output beam taken with a pyroelectric camera, the Gaussian-like mode content of the output beam was computed to be 92% with an ellipticity of 12%. PMID:21243088

  7. Continuous-Wave Operation of a Frequency-Tunable 460-GHz Second-Harmonic Gyrotron for Enhanced Nuclear Magnetic Resonance.

    PubMed

    Torrezan, Antonio C; Han, Seong-Tae; Mastovsky, Ivan; Shapiro, Michael A; Sirigiri, Jagadishwar R; Temkin, Richard J; Griffin, Robert G; Barnes, Alexander B

    2010-06-01

    The design, operation, and characterization of a continuous-wave (CW) tunable second-harmonic 460-GHz gyrotron are reported. The gyrotron is intended to be used as a submillimeter-wave source for 700-MHz nuclear magnetic resonance experiments with sensitivity enhanced by dynamic nuclear polarization. The gyrotron operates in the whispering-gallery mode TE11,2 and has generated 16 W of output power with a 13-kV 100-mA electron beam. The start oscillation current measured over a range of magnetic field values is in good agreement with theoretical start currents obtained from linear theory for successive high-order axial modes TE11,2,q. The minimum start current is 27 mA. Power and frequency tuning measurements as a function of the electron cyclotron frequency have also been carried out. A smooth frequency tuning range of 1 GHz was obtained for the operating second-harmonic mode either by magnetic field tuning or beam voltage tuning. Long-term CW operation was evaluated during an uninterrupted period of 48 h, where the gyrotron output power and frequency were kept stable to within ±0.7% and ±6 ppm, respectively, by a computerized control system. Proper operation of an internal quasi-optical mode converter implemented to transform the operating whispering-gallery mode to a Gaussian-like beam was also verified. Based on the images of the gyrotron output beam taken with a pyroelectric camera, the Gaussian-like mode content of the output beam was computed to be 92% with an ellipticity of 12%. PMID:23761938

  8. Continuous-Wave Operation of a Frequency-Tunable 460-GHz Second-Harmonic Gyrotron for Enhanced Nuclear Magnetic Resonance

    PubMed Central

    Torrezan, Antonio C.; Han, Seong-Tae; Mastovsky, Ivan; Shapiro, Michael A.; Sirigiri, Jagadishwar R.; Temkin, Richard J.; Barnes, Alexander B.; Griffin, Robert G.

    2011-01-01

    The design, operation, and characterization of a continuous-wave (CW) tunable second-harmonic 460-GHz gyrotron are reported. The gyrotron is intended to be used as a submillimeter-wave source for 700-MHz nuclear magnetic resonance experiments with sensitivity enhanced by dynamic nuclear polarization. The gyrotron operates in the whispering-gallery mode TE11,2 and has generated 16 W of output power with a 13-kV 100-mA electron beam. The start oscillation current measured over a range of magnetic field values is in good agreement with theoretical start currents obtained from linear theory for successive high-order axial modes TE11,2,q. The minimum start current is 27 mA. Power and frequency tuning measurements as a function of the electron cyclotron frequency have also been carried out. A smooth frequency tuning range of 1 GHz was obtained for the operating second-harmonic mode either by magnetic field tuning or beam voltage tuning. Long-term CW operation was evaluated during an uninterrupted period of 48 h, where the gyrotron output power and frequency were kept stable to within ±0.7% and ±6 ppm, respectively, by a computerized control system. Proper operation of an internal quasi-optical mode converter implemented to transform the operating whispering-gallery mode to a Gaussian-like beam was also verified. Based on the images of the gyrotron output beam taken with a pyroelectric camera, the Gaussian-like mode content of the output beam was computed to be 92% with an ellipticity of 12%. PMID:21243088

  9. Continuous-Wave Operation of a Frequency-Tunable 460-GHz Second-Harmonic Gyrotron for Enhanced Nuclear Magnetic Resonance

    PubMed Central

    Torrezan, Antonio C.; Han, Seong-Tae; Mastovsky, Ivan; Shapiro, Michael A.; Sirigiri, Jagadishwar R.; Temkin, Richard J.; Griffin, Robert G.; Barnes, Alexander B.

    2012-01-01

    The design, operation, and characterization of a continuous-wave (CW) tunable second-harmonic 460-GHz gyrotron are reported. The gyrotron is intended to be used as a submillimeter-wave source for 700-MHz nuclear magnetic resonance experiments with sensitivity enhanced by dynamic nuclear polarization. The gyrotron operates in the whispering-gallery mode TE11,2 and has generated 16 W of output power with a 13-kV 100-mA electron beam. The start oscillation current measured over a range of magnetic field values is in good agreement with theoretical start currents obtained from linear theory for successive high-order axial modes TE11,2,q. The minimum start current is 27 mA. Power and frequency tuning measurements as a function of the electron cyclotron frequency have also been carried out. A smooth frequency tuning range of 1 GHz was obtained for the operating second-harmonic mode either by magnetic field tuning or beam voltage tuning. Long-term CW operation was evaluated during an uninterrupted period of 48 h, where the gyrotron output power and frequency were kept stable to within ±0.7% and ±6 ppm, respectively, by a computerized control system. Proper operation of an internal quasi-optical mode converter implemented to transform the operating whispering-gallery mode to a Gaussian-like beam was also verified. Based on the images of the gyrotron output beam taken with a pyroelectric camera, the Gaussian-like mode content of the output beam was computed to be 92% with an ellipticity of 12%. PMID:23761938

  10. A 0.33-THz second-harmonic frequency-tunable gyrotron

    NASA Astrophysics Data System (ADS)

    Zheng-Di, Li; Chao-Hai, Du; Xiang-Bo, Qi; Li, Luo; Pu-Kun, Liu

    2016-02-01

    Dynamics of the axial mode transition process in a 0.33-THz second-harmonic gyrotron is investigated to reveal the physical mechanism of realizing broadband frequency tuning in an open cavity circuit. A new interaction mechanism about propagating waves, featured by wave competition and wave cooperation, is presented and provides a new insight into the beam-wave interaction. The two different features revealed in the two different operation regions of low-order axial modes (LOAMs) and high-order axial modes (HOAMs) respectively determine the characteristic of the overall performance of the device essentially. The device performance is obtained by the simulation based on the time-domain nonlinear theory and shows that using a 12-kV/150-mA electron beam and TE-3,4 mode, the second harmonic gyrotron can generate terahertz radiations with frequency-tuning ranges of about 0.85 GHz and 0.60 GHz via magnetic field and beam voltage tuning, respectively. Additionally, some non-stationary phenomena in the mode startup process are also analyzed. The investigation in this paper presents guidance for future developing high-performance frequency-tunable gyrotrons toward terahertz applications. Project supported by the National Natural Science Foundation of China (Grant Nos. 61471007, 61531002, 61522101, and 11275206) and the Seeding Grant for Medicine and Information Science of Peking University, China (Grant No. 2014-MI-01).

  11. Single pulse frequency compounding protocol for superharmonic imaging

    NASA Astrophysics Data System (ADS)

    Danilouchkine, Mikhail G.; van Neer, Paul L. M. J.; Matte, Guillaume M.; Verweij, Martin D.; de Jong, Nico

    2011-03-01

    Second harmonic imaging is currently adopted as standard in commercial echographic systems. A new imaging technique, coined as superharmonic imaging (SHI), combines the 3rd till the 5th harmonics, arising during nonlinear sound propagation. It could further enhance resolution and quality of echographic images. To meet the bandwidth requirement for SHI a dedicated phased array has been developed: a low frequency subarray, intended for transmission, interleaved with a high frequency subarray, used in reception. As the bandwidth of the elements is limited, the spectral gaps in between the harmonics cause multiple reflection artifacts. Recently, we introduce a dual-pulse frequency compounding (DPFC) method to suppress those artifacts at price of a reduced frame rate. In this study we investigate the feasibility of performing the frequency compounding protocol within a single transmission. The traditional DPFC method constructs each trace in a post-processing stage by summing echoes from two emitted pulses, the second slightly frequency-shifted compared to the first. In the newly proposed method, the transmit aperture is divided into two parts: the first half is used to send a pulse at the lower center frequency, while the other half simultaneously transmits at the higher center frequency. The suitability of the protocol for medical imaging applications in terms of the steering capabilities was performed in a simulation study using the FIELD II toolkit. Moreover, an experimental study was performed to deduce the optimal parametric set for implementation of the clinical imaging protocol. The latter was subsequently used to obtain the images of a tissue mimicking phantom containing strongly reflecting wires. For in-vitro acquisitions the SHI probe with interleaved phased array (44 odd elements at 1MHz and 44 even elements at 3.7MHz elements, optimized for echocardiography) was connected to a fully programmable ultrasound system. The results of the Field II simulations

  12. A piezoelectric pulse generator for low frequency non-harmonic vibration

    NASA Astrophysics Data System (ADS)

    Jiang, Hao; Yeatman, Eric M.

    2013-12-01

    This paper reports a new piezoelectric prototype for pulse generation by energy harvesting from low frequency non-harmonic vibration. The pulse generator presented here consists of two parts: the electromechanical part and the load circuit. A metal rolling rod is used as the proof mass, moving along the substrate to achieve both actuating of the piezoelectric cantilever by magnetic coupling and self-synchronous switching of the circuit. By using this new approach, the energy from the piezoelectric transduction mechanism is regulated simultaneously when it is extracted. This allows a series of tuneable pulses to be generated, which can be applied to self-powered RF wireless sensor network (WSN) nodes.

  13. Simultaneous negative refraction and focusing of fundamental frequency and second-harmonic fields by two-dimensional photonic crystals

    SciTech Connect

    Zhang, Jun; Zhang, Xiangdong

    2015-09-28

    Simultaneous negative refraction for both the fundamental frequency (FF) and second-harmonic (SH) fields in two-dimensional nonlinear photonic crystals have been found through both the physical analysis and exact numerical simulation. By combining such a property with the phase-matching condition and strong second-order susceptibility, we have designed a SH lens to realize focusing for both the FF and SH fields at the same time. Good-quality non-near field images for both FF and SH fields have been observed. The physical mechanism for such SH focusing phenomena has been disclosed, which is different from the backward SH generation as has been pointed out in the previous investigations. In addition, the effect of absorption losses on the phenomena has also been discussed. Thus, potential applications of these phenomena to biphotonic microscopy technique are anticipated.

  14. Improvement of Shear Wave Motion Detection Using Harmonic Imaging in Healthy Human Liver.

    PubMed

    Amador, Carolina; Song, Pengfei; Meixner, Duane D; Chen, Shigao; Urban, Matthew W

    2016-05-01

    Quantification of liver elasticity is a major application of shear wave elasticity imaging (SWEI) to non-invasive assessment of liver fibrosis stages. SWEI measurements can be highly affected by ultrasound image quality. Ultrasound harmonic imaging has exhibited a significant improvement in ultrasound image quality as well as for SWEI measurements. This was previously illustrated in cardiac SWEI. The purpose of this study was to evaluate liver shear wave particle displacement detection and shear wave velocity (SWV) measurements with fundamental and filter-based harmonic ultrasound imaging. In a cohort of 17 patients with no history of liver disease, a 2.9-fold increase in maximum shear wave displacement, a 0.11 m/s decrease in the overall interquartile range and median SWV and a 17.6% increase in the success rate of SWV measurements were obtained when filter-based harmonic imaging was used instead of fundamental imaging. PMID:26803391

  15. The electromagnetic environment of Magnetic Resonance Imaging systems. Occupational exposure assessment reveals RF harmonics

    NASA Astrophysics Data System (ADS)

    Gourzoulidis, G.; Karabetsos, E.; Skamnakis, N.; Kappas, C.; Theodorou, K.; Tsougos, I.; Maris, T. G.

    2015-09-01

    Magnetic Resonance Imaging (MRI) systems played a crucial role in the postponement of the former occupational electromagnetic fields (EMF) European Directive (2004/40/EC) and in the formation of the latest exposure limits adopted in the new one (2013/35/EU). Moreover, the complex MRI environment will be finally excluded from the implementation of the new occupational limits, leading to an increased demand for Occupational Health and Safety (OHS) surveillance. The gradient function of MRI systems and the application of the RF excitation frequency result in low and high frequency exposures, respectively. This electromagnetic field exposure, in combination with the increased static magnetic field exposure, makes the MRI environment a unique case of combined EMF exposure. The electromagnetic field levels in close proximity of different MRI systems have been assessed at various frequencies. Quality Assurance (QA) & safety issues were also faced. Preliminary results show initial compliance with the forthcoming limits in each different frequency band, but also revealed peculiar RF harmonic components, of no safety concern, to the whole range detected (20-1000MHz). Further work is needed in order to clarify their origin and characteristics.

  16. Phenomena induced by powerful HF pumping towards magnetic zenith with a frequency near the F-region critical frequency and the third electron gyro harmonic frequency

    NASA Astrophysics Data System (ADS)

    Blagoveshchenskaya, N. F.; Carlson, H. C.; Kornienko, V. A.; Borisova, T. D.; Rietveld, M. T.; Yeoman, T. K.; Brekke, A.

    2009-01-01

    Multi-instrument observational data from an experiment on 13 October 2006 at the EISCAT/HEATING facility at Tromsø, Norway are analysed. The experiment was carried out in the evening hours when the electron density in the F-region dropped, and the HF pump frequency fH was near and then above the critical frequency of the F2 layer. The distinctive feature of this experiment is that the pump frequency was just below the third electron gyro harmonic frequency, while both the HF pump beam and UHF radar beam were directed towards the magnetic zenith (MZ). The HF pump-induced phenomena were diagnosed with several instruments: the bi-static HF radio scatter on the London-Tromsø-St. Petersburg path, the CUTLASS radar in Hankasalmi (Finland), the European Incoherent Scatter (EISCAT) UHF radar at Tromsø and the Tromsø ionosonde (dynasonde). The results show thermal electron excitation of the HF-induced striations seen simultaneously from HF bi-static scatter and CUTLASS radar observations, accompanied by increases of electron temperature when the heater frequency was near and then above the critical frequency of the F2 layer by up to 0.4 MHz. An increase of the electron density up to 25% accompanied by strong HF-induced electron heating was observed, only when the heater frequency was near the critical frequency and just below the third electron gyro harmonic frequency. It is concluded that the combined effect of upper hybrid resonance and gyro resonance at the same altitude gives rise to strong electron heating, the excitation of striations, HF ray trapping and extension of HF waves to altitudes where they can excite Langmuir turbulence and fluxes of electrons accelerated to energies that produce ionization.

  17. Conformation, orientation and interaction in molecular monolayers: A surface second harmonic and sum frequency generation study

    SciTech Connect

    Superfine, R.; Huang, J.Y.; Shen, Y.R.

    1988-12-01

    We have used sum frequency generation (SFG) to study the order in a silane monolayer before and after the deposition of a coadsorbed liquid crystal monolayer. We observe an increase in the order of the chain of the silane molecule induced by the interpenetration of the liquid crystal molecules. By using second harmonic generation (SHG) and SFG, we have studied the orientation and conformation of the liquid crystal molecule on clean and silane coated glass surfaces. On both surfaces, the biphenyl group is tilted by 70{degree} with the alkyl chain end pointing away from the surface. The shift in the C-H stretch frequencies in the coadsorbed system indicates a significant interaction between molecules. 9 refs., 3 figs.

  18. Protection of VHF international distress frequencies from harmonic radiation due to digital television equipment

    NASA Astrophysics Data System (ADS)

    Middleton, J.

    Digital television picture processing equipment uses a luminance sampling frequency of 13.5 MHz, which can give rise to harmonics at 121.5 and 243 MHz. If such equipment becomes sufficiently widespread as will probably be the case with MAC/packet receivers, it is possible that the cumulative radiation could become significantly high. Since these frequencies are used by the international distress services there is a potential for interference if this unwanted radiation is not controlled at the point of manufacture. Over the last two years this problem was studied by the BBC in conjunction with the EBU. The conclusion is that the distress services will be protected provided that digital television picture processing equipment meets existing electromagnetic compatibility (EMC) standards for information technology equipment. In the case of domestic television this should not present a problem, but for studio equipment, because of its size and complexity, EMC compliance may not be so easy.

  19. Optimization of contrast resolution by genetic algorithm in ultrasound tissue harmonic imaging.

    PubMed

    Ménigot, Sébastien; Girault, Jean-Marc

    2016-09-01

    The development of ultrasound imaging techniques such as pulse inversion has improved tissue harmonic imaging. Nevertheless, no recommendation has been made to date for the design of the waveform transmitted through the medium being explored. Our aim was therefore to find automatically the optimal "imaging" wave which maximized the contrast resolution without a priori information. To overcome assumption regarding the waveform, a genetic algorithm investigated the medium thanks to the transmission of stochastic "explorer" waves. Moreover, these stochastic signals could be constrained by the type of generator available (bipolar or arbitrary). To implement it, we changed the current pulse inversion imaging system by including feedback. Thus the method optimized the contrast resolution by adaptively selecting the samples of the excitation. In simulation, we benchmarked the contrast effectiveness of the best found transmitted stochastic commands and the usual fixed-frequency command. The optimization method converged quickly after around 300 iterations in the same optimal area. These results were confirmed experimentally. In the experimental case, the contrast resolution measured on a radiofrequency line could be improved by 6% with a bipolar generator and it could still increase by 15% with an arbitrary waveform generator. PMID:27403642

  20. Fully automated muscle quality assessment by Gabor filtering of second harmonic generation images

    NASA Astrophysics Data System (ADS)

    Paesen, Rik; Smolders, Sophie; Vega, José Manolo de Hoyos; Eijnde, Bert O.; Hansen, Dominique; Ameloot, Marcel

    2016-02-01

    Although structural changes on the sarcomere level of skeletal muscle are known to occur due to various pathologies, rigorous studies of the reduced sarcomere quality remain scarce. This can possibly be explained by the lack of an objective tool for analyzing and comparing sarcomere images across biological conditions. Recent developments in second harmonic generation (SHG) microscopy and increasing insight into the interpretation of sarcomere SHG intensity profiles have made SHG microscopy a valuable tool to study microstructural properties of sarcomeres. Typically, sarcomere integrity is analyzed by fitting a set of manually selected, one-dimensional SHG intensity profiles with a supramolecular SHG model. To circumvent this tedious manual selection step, we developed a fully automated image analysis procedure to map the sarcomere disorder for the entire image at once. The algorithm relies on a single-frequency wavelet-based Gabor approach and includes a newly developed normalization procedure allowing for unambiguous data interpretation. The method was validated by showing the correlation between the sarcomere disorder, quantified by the M-band size obtained from manually selected profiles, and the normalized Gabor value ranging from 0 to 1 for decreasing disorder. Finally, to elucidate the applicability of our newly developed protocol, Gabor analysis was used to study the effect of experimental autoimmune encephalomyelitis on the sarcomere regularity. We believe that the technique developed in this work holds great promise for high-throughput, unbiased, and automated image analysis to study sarcomere integrity by SHG microscopy.

  1. Second Harmonic Imaging improves Echocardiograph Quality on board the International Space Station

    NASA Technical Reports Server (NTRS)

    Garcia, Kathleen; Sargsyan, Ashot; Hamilton, Douglas; Martin, David; Ebert, Douglas; Melton, Shannon; Dulchavsky, Scott

    2008-01-01

    Ultrasound (US) capabilities have been part of the Human Research Facility (HRF) on board the International Space Station (ISS) since 2001. The US equipment on board the ISS includes a first-generation Tissue Harmonic Imaging (THI) option. Harmonic imaging (HI) is the second harmonic response of the tissue to the ultrasound beam and produces robust tissue detail and signal. Since this is a first-generation THI, there are inherent limitations in tissue penetration. As a breakthrough technology, HI extensively advanced the field of ultrasound. In cardiac applications, it drastically improves endocardial border detection and has become a common imaging modality. U.S. images were captured and stored as JPEG stills from the ISS video downlink. US images with and without harmonic imaging option were randomized and provided to volunteers without medical education or US skills for identification of endocardial border. The results were processed and analyzed using applicable statistical calculations. The measurements in US images using HI improved measurement consistency and reproducibility among observers when compared to fundamental imaging. HI has been embraced by the imaging community at large as it improves the quality and data validity of US studies, especially in difficult-to-image cases. Even with the limitations of the first generation THI, HI improved the quality and measurability of many of the downlinked images from the ISS and should be an option utilized with cardiac imaging on board the ISS in all future space missions.

  2. High-resolution harmonic motion imaging (HR-HMI) for tissue biomechanical property characterization

    PubMed Central

    Ma, Teng; Qian, Xuejun; Chiu, Chi Tat; Yu, Mingyue; Jung, Hayong; Tung, Yao-Sheng; Shung, K. Kirk

    2015-01-01

    Background Elastography, capable of mapping the biomechanical properties of biological tissues, serves as a useful technique for clinicians to perform disease diagnosis and determine stages of many diseases. Many acoustic radiation force (ARF) based elastography, including acoustic radiation force impulse (ARFI) imaging and harmonic motion imaging (HMI), have been developed to remotely assess the elastic properties of tissues. However, due to the lower operating frequencies of these approaches, their spatial resolutions are insufficient for revealing stiffness distribution on small scale applications, such as cancerous tumor margin detection, atherosclerotic plaque composition analysis and ophthalmologic tissue characterization. Though recently developed ARF-based optical coherence elastography (OCE) methods open a new window for the high resolution elastography, shallow imaging depths significantly limit their usefulness in clinics. Methods The aim of this study is to develop a high-resolution HMI method to assess the tissue biomechanical properties with acceptable field of view (FOV) using a 4 MHz ring transducer for efficient excitation and a 40 MHz needle transducer for accurate detection. Under precise alignment of two confocal transducers, the high-resolution HMI system has a lateral resolution of 314 µm and an axial resolution of 
147 µm with an effective FOV of 2 mm in depth. Results The performance of this high resolution imaging system was validated on the agar-based tissue mimicking phantoms with different stiffness distributions. These data demonstrated the imaging system’s improved resolution and sensitivity on differentiating materials with varying stiffness. In addition, ex vivo imaging of a human atherosclerosis coronary artery demonstrated the capability of high resolution HMI in identifying layer-specific structures and characterizing atherosclerotic plaques based on their stiffness differences. Conclusions All together high resolution HMI

  3. Mosquito (Aedes aegypti) flight tones: Frequency, harmonicity, spherical spreading, and phase relationships

    PubMed Central

    Arthur, Benjamin J.; Emr, Kevin S.; Wyttenbach, Robert A.; Hoy, Ronald R.

    2014-01-01

    Mosquito flight produces a tone as a side effect of wing movement; this tone is also a communication signal that is frequency-modulated during courtship. Recordings of tones produced by tethered flying male and female Aedes aegypti were undertaken using pairs of pressure-gradient microphones above and below, ahead and behind, and to the left and right over a range of distances. Fundamental frequencies were close to those previously reported, although amplitudes were lower. The male fundamental frequency was higher than that of the female and males modulated it over a wider range. Analysis of harmonics shows that the first six partials were nearly always within 1 Hz of integer multiples of the fundamental, even when the fundamental was being modulated. Along the front-back axis, amplitude attenuated as a function of distance raised to the power 2.3. Front and back recordings were out of phase, as were above and below, while left and right were in phase. Recordings from ahead and behind showed quadratic phase coupling, while others did not. Finally, two methods are presented for separating simultaneous flight tones in a single recording and enhancing their frequency resolution. Implications for mosquito behavior are discussed. PMID:25234901

  4. Imaging the bone marrow stem cells morphogenesis in PGA scaffold by multiphoton autofluorescence and second harmonic (SHG) imaging

    NASA Astrophysics Data System (ADS)

    Lee, Hsuan-Shu; Teng, Shu-Wen; Chen, Hsiao-Ching; Lo, Wen; Sun, Yen; Lin, Tze-Yu; Chiou, Ling-Ling; Jiang, Ching-Chuan; Dong, Chen-Yuan

    2006-02-01

    The ability to image tissue engineering products without damaging histological procedures is important for the understanding of the dynamics of tissue reorganization and formation. In this work, we test the ability of multiphoton autofluorescence and second harmonic generation microscopy to image engineered tissues following chrondrogenic induction. The system we used is human bone marrow stem cells seeded in the scaffold polyglycolic acid (PGA). Our results show that autofluorescence can be used to image cells while second harmonic generation signal can be used to visualize the synthesis of extracellular matrix. This approach demonstrates the ability of multiphoton imaging in the study of tissue engineering products.

  5. Squeezing induced in a harmonic oscillator by a sudden change in mass or frequency

    NASA Astrophysics Data System (ADS)

    Abdalla, M. Sebawe; Colegrave, R. K.

    1993-08-01

    The Kanai-Caldirola (Bateman) Hamiltonian is used to derive the dynamics of a simple harmonic oscillator, initially in a minimum uncertainty state, under the influence of an external agency which causes the mass parameter to change from M0 to M1 in a short time ɛ. Then the frequency changes from ω0 to ω1=(M0/M1)ω0+O(ɛ2). In the limit ɛ-->0, no squeezing or loss of coherence occurs. If M1/M0=1+/-η (0<η<<1), then a squeezing of order ɛ2η occurs. If M1/M0 is appreciably different from unity, then the quadrature variances are unequal but the state no longer has minimum uncertainty. An application could be made in quantum optics.

  6. Measurement of sound velocity made easy using harmonic resonant frequencies with everyday mobile technology

    NASA Astrophysics Data System (ADS)

    Hirth, Michael; Kuhn, Jochen; Müller, Andreas

    2015-02-01

    Recent articles about smartphone experiments have described their applications as experimental tools in different physical contexts.1-4 They have established that smartphones facilitate experimental setups, thanks to the small size and diverse functions of mobile devices, in comparison to setups with computer-based measurements. In the experiment described in this article, the experimental setup is reduced to a minimum. The objective of the experiment is to determine the speed of sound with a high degree of accuracy using everyday tools. An article published recently proposes a time-of-flight method where sound or acoustic pulses are reflected at the ends of an open tube.5 In contrast, the following experiment idea is based on the harmonic resonant frequencies of such a tube, simultaneously triggered by a noise signal.

  7. A Preliminary Engineering Design of Intravascular Dual-Frequency Transducers for Contrast-Enhanced Acoustic Angiography and Molecular Imaging

    PubMed Central

    Ma, Jianguo; Martin, K. Heath; Dayton, Paul A.; Jiang, Xiaoning

    2014-01-01

    Current intravascular ultrasound (IVUS) probes are not optimized for contrast detection because of their design for high-frequency fundamental-mode imaging. However, data from transcutaneous contrast imaging suggests the possibility of utilizing contrast ultrasound for molecular imaging or vasa vasorum assessment to further elucidate atherosclerotic plaque deposition. This paper presents the design, fabrication, and characterization of a small-aperture (0.6 × 3 mm) IVUS probe optimized for high-frequency contrast imaging. The design utilizes a dual-frequency (6.5 MHz/30 MHz) transducer arrangement for exciting microbubbles at low frequencies (near their resonance) and detecting their broadband harmonics at high frequencies, minimizing detected tissue backscatter. The prototype probe is able to generate nonlinear microbubble response with more than 1.2 MPa of rarefractional pressure (mechanical index: 0.48) at 6.5 MHz, and is also able to detect microbubble response with a broadband receiving element (center frequency: 30 MHz, −6-dB fractional bandwidth: 58.6%). Nonlinear super-harmonics from microbubbles flowing through a 200-μm-diameter micro-tube were clearly detected with a signal-to-noise ratio higher than 12 dB. Preliminary phantom imaging at the fundamental frequency (30 MHz) and dual-frequency super-harmonic imaging results suggest the promise of small aperture, dual-frequency IVUS transducers for contrast-enhanced IVUS imaging. PMID:24801226

  8. MREIT conductivity imaging based on the local harmonic Bz algorithm: Animal experiments

    NASA Astrophysics Data System (ADS)

    Jeon, Kiwan; Lee, Chang-Ock; Woo, Eung Je; Kim, Hyung Joong; Seo, Jin Keun

    2010-04-01

    From numerous numerical and phantom experiments, MREIT conductivity imaging based on harmonic Bz algorithm shows that it could be yet another useful medical imaging modality. However, in animal experiments, the conventional harmonic Bz algorithm gives poor results near boundaries of problematic regions such as bones, lungs, and gas-filled stomach, and the subject boundary where electrodes are not attached. Since the amount of injected current is low enough for the safety for in vivo animal, the measured Bz data is defected by severe noise. In order to handle such problems, we use the recently developed local harmonic Bz algorithm to obtain conductivity images in our ROI(region of interest) without concerning the defected regions. Furthermore we adopt a denoising algorithm that preserves the ramp structure of Bz data, which informs of the location and size of anomaly. Incorporating these efficient techniques, we provide the conductivity imaging of post-mortem and in vivo animal experiments with high spatial resolution.

  9. In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy.

    PubMed

    Gualda, E J; Filippidis, G; Mari, M; Voglis, G; Vlachos, M; Fotakis, C; Tavernarakis, N

    2008-11-01

    In this study, neurodegeneration phenomena were investigated, by performing third harmonic generation imaging measurements on the nematode Caenorhabditis elegans, in vivo. The in vivo, precise identification of the contour of the degenerating neurons in the posterior part of the nematode and the monitoring, in real time, of the progression of degeneration in the worm, through third harmonic generation imaging measurements, were achieved. Femtosecond laser pulses (1028 nm) were utilized for excitation. Thus, the THG image contrast modality comprises a powerful diagnostic tool, providing valuable information and offering new insights into morphological changes and complex developmental processes in live biological specimens. PMID:19017226

  10. Floquet topological system based on frequency-modulated classical coupled harmonic oscillators

    NASA Astrophysics Data System (ADS)

    Salerno, Grazia; Ozawa, Tomoki; Price, Hannah M.; Carusotto, Iacopo

    2016-02-01

    We theoretically propose how to observe topological effects in a generic classical system of coupled harmonic oscillators, such as classical pendula or lumped-element electric circuits, whose oscillation frequency is modulated fast in time. Making use of Floquet theory in the high-frequency limit, we identify a regime in which the system is accurately described by a Harper-Hofstadter model where the synthetic magnetic field can be externally tuned via the phase of the frequency modulation of the different oscillators. We illustrate how the topologically protected chiral edge states, as well as the Hofstadter butterfly of bulk bands, can be observed in the driven-dissipative steady state under a monochromatic drive. In analogy with the integer quantum Hall effect, we show how the topological Chern numbers of the bands can be extracted from the mean transverse shift of the steady-state oscillation amplitude distribution. Finally, we discuss the regime where the analogy with the Harper-Hofstadter model breaks down.

  11. Frequency-comb formation in doubly resonant second-harmonic generation

    NASA Astrophysics Data System (ADS)

    Leo, F.; Hansson, T.; Ricciardi, I.; De Rosa, M.; Coen, S.; Wabnitz, S.; Erkintalo, M.

    2016-04-01

    We theoretically study the generation of optical frequency combs and corresponding pulse trains in doubly resonant intracavity second-harmonic generation (SHG). We find that, despite the large temporal walk-off characteristic of realistic cavity systems, the nonlinear dynamics can be accurately and efficiently modeled using a pair of coupled mean-field equations. Through rigorous stability analysis of the system's steady-state continuous-wave solutions, we demonstrate that walk-off can give rise to an unexplored regime of temporal modulation instability. Numerical simulations performed in this regime reveal rich dynamical behaviors, including the emergence of temporal patterns that correspond to coherent optical frequency combs. We also demonstrate that the two coupled equations that govern the doubly resonant cavity behavior can, under typical conditions, be reduced to a single mean-field equation akin to that describing the dynamics of singly-resonant-cavity SHG [F. Leo et al., Phys. Rev. Lett. 116, 033901 (2016), 10.1103/PhysRevLett.116.033901]. This reduced approach allows us to derive a simple expression for the modulation instability gain, thus permitting us to acquire significant insight into the underlying physics. We anticipate that our work will have a wide impact on the study of frequency combs in emerging doubly resonant cavity SHG platforms, including quadratically nonlinear microresonators.

  12. Quadrupole second harmonic generation and sum-frequency generation in ZnO quantum dots

    SciTech Connect

    Maikhuri, Deepti; Purohit, S. P. Mathur, K. C.

    2015-04-15

    The second harmonic generation (SHG) and the sum frequency generation (SFG) processes are investigated in the conduction band states of the singly charged ZnO quantum dot (QD) embedded in the HfO{sub 2}, and the AlN matrices. With two optical fields of frequency ω{sub p} and ω{sub q} incident on the dot, we study the variation with frequency of the second order nonlinear polarization resulting in SHG and SFG, through the electric dipole and the electric quadrupole interactions of the pump fields with the electron in the dot. We obtain enhanced value of the second order nonlinear susceptibility in the dot compared to the bulk. The effective mass approximation with the finite confining barrier is used for obtaining the energy and wavefunctions of the quantized confined states of the electron in the conduction band of the dot. Our results show that both the SHG and SFG processes depend on the dot size, the surrounding matrix and the polarization states of the pump beams.

  13. Dual Frequency Coil Array for MR Imaging

    NASA Astrophysics Data System (ADS)

    Amador-Baheza, R.; Sacristan-Rock, E.; Rodríguez, A. O.

    2002-08-01

    An array coil to perform in vivo Magnetic Resonance Imaging and Spectroscopy was developed to study the intestinal wall. It consisted of two surface rectangular-shaped coils mounted on cylindrical structure forming an orthogonal assembly. Since this design is intended to generate images and spectra, each element was tuned to a different resonant frequency: a) imaging: 200 MHz (1H) and b) spectroscopy: 81 MHz (31P). However, at this stage of the research, imaging experiments were only conducted on a Bruker 4.7 Tesla animal system. High-resolution images were obtained from a saline filled phantom and from the intestinal wall of a fully anaesthetised rabbit. The dual frequency coil array can be used to study the pathophysiology of intestinal ischemia.

  14. Characteristics of different frequency ranges in scanning electron microscope images

    SciTech Connect

    Sim, K. S. Nia, M. E.; Tan, T. L.; Tso, C. P.; Ee, C. S.

    2015-07-22

    We demonstrate a new approach to characterize the frequency range in general scanning electron microscope (SEM) images. First, pure frequency images are generated from low frequency to high frequency, and then, the magnification of each type of frequency image is implemented. By comparing the edge percentage of the SEM image to the self-generated frequency images, we can define the frequency ranges of the SEM images. Characterization of frequency ranges of SEM images benefits further processing and analysis of those SEM images, such as in noise filtering and contrast enhancement.

  15. Ab Initio Theoretical Investigation of the Frequency Comb Structure in the XUV Regime via High Harmonic Generation

    NASA Astrophysics Data System (ADS)

    Carrera, Juan J.; Son, Sang-Kil; Chu, Shih-I.

    2007-06-01

    We present an ab initio quantum investigation of the frequency comb structure formed within each high harmonic generation (HHG) power spectrum driven by a train of equal- spacing short laser pulses. The HHG power spectrum of atomic hydrogen is calculated by solving the time-dependent Schr"o dinger equation accurately and efficiently by means of the time- dependent generalized pseudospectral method. We found that the frequency comb structure is preserved within each harmonic. In addition, the repetition frequency of the comb laser depends upon the pulse separation τ and the spectral width of each individual comb fringe is inversely proportional to the number of pulses (n) used. However, the global HHG power spectrum pattern depends only upon the laser frequency and intensity used and is not sensitive to the τ and n parameters. Finally, the frequency comb structure persists even in the presence of appreciable ionization.

  16. Frequency Domain Sampling Using Biomedical Imaging Physics

    NASA Astrophysics Data System (ADS)

    Seo, Gun Ha; Chung, Minji; Kyung, Richard

    2015-04-01

    In magnetic resonance image analysis using physical and computational method, the process of transformation from frequency domain to image domain requires significant amount time because Inverse Fourier Transformation (IFT) takes every frequency points to determine the final output image. This paper shows the mechanisms and physics of image formation using the selectivity of proper k-space by removing different amounts of high or low frequencies to create the most optimal magnetic resonance image of a human tibial bone. Originally, square unit step function, N/2-N/10:N/2 + N/10 = 1, was used during the Fourier Transformations. And Gaussian filter, y = exp(-t2/40n) , where t = h-L/2, h = [0,M], L =2*7*N/40, the size of frequency matrix (M, N) = (365,557) was tested. Also circle equations as a filter, r = sqrt((x-M/2)2 + (y-N/2)2) , were tested in creating the images of the human tibial bone to find an efficient filter. The best efficiency occurred when the exponent n in the proposed Gaussian filter equation is in between 3 and 8, and therefore, a new algorithm is needed to find the exact number since the number is not only an integer.

  17. Definitions of non-stationary vibration power for time-frequency analysis and computational algorithms based upon harmonic wavelet transform

    NASA Astrophysics Data System (ADS)

    Heo, YongHwa; Kim, Kwang-joon

    2015-02-01

    While the vibration power for a set of harmonic force and velocity signals is well defined and known, it is not as popular yet for a set of stationary random force and velocity processes, although it can be found in some literatures. In this paper, the definition of the vibration power for a set of non-stationary random force and velocity signals will be derived for the purpose of a time-frequency analysis based on the definitions of the vibration power for the harmonic and stationary random signals. The non-stationary vibration power, defined as the short-time average of the product of the force and velocity over a given frequency range of interest, can be calculated by three methods: the Wigner-Ville distribution, the short-time Fourier transform, and the harmonic wavelet transform. The latter method is selected in this paper because band-pass filtering can be done without phase distortions, and the frequency ranges can be chosen very flexibly for the time-frequency analysis. Three algorithms for the time-frequency analysis of the non-stationary vibration power using the harmonic wavelet transform are discussed. The first is an algorithm for computation according to the full definition, while the others are approximate. Noting that the force and velocity decomposed into frequency ranges of interest by the harmonic wavelet transform are constructed with coefficients and basis functions, for the second algorithm, it is suggested to prepare a table of time integrals of the product of the basis functions in advance, which are independent of the signals under analysis. How to prepare and utilize the integral table are presented. The third algorithm is based on an evolutionary spectrum. Applications of the algorithms to the time-frequency analysis of the vibration power transmitted from an excitation source to a receiver structure in a simple mechanical system consisting of a cantilever beam and a reaction wheel are presented for illustration.

  18. Multi-Frequency Intravascular Ultrasound (IVUS) Imaging

    PubMed Central

    Ma, Teng; Yu, Mingyue; Chen, Zeyu; Fei, Chunlong; Shung, K. Kirk; Zhou, Qifa

    2015-01-01

    Acute coronary syndrome (ACS) is frequently associated with the sudden rupture of a vulnerable atherosclerotic plaque within the coronary artery. Several unique physiological features, including a thin fibrous cap accompanied by a necrotic lipid core, are the targeted indicators for identifying the vulnerable plaques. Intravascular ultrasound (IVUS), a catheter-based imaging technology, has been routinely performed in clinics for more than 20 years to describe the morphology of the coronary artery and guide percutaneous coronary interventions. However, conventional IVUS cannot facilitate the risk assessment of ACS because of its intrinsic limitations, such as insufficient resolution. Renovation of the IVUS technology is essentially needed to overcome the limitations and enhance the coronary artery characterization. In this paper, a multi-frequency intravascular ultrasound (IVUS) imaging system was developed by incorporating a higher frequency IVUS transducer (80 to 150 MHz) with the conventional IVUS (30–50 MHz) system. The newly developed system maintains the advantage of deeply penetrating imaging with the conventional IVUS, while offering an improved higher resolution image with IVUS at a higher frequency. The prototyped multi-frequency catheter has a clinically compatible size of 0.95 mm and a favorable capability of automated image co-registration. In vitro human coronary artery imaging has demonstrated the feasibility and superiority of the multi-frequency IVUS imaging system to deliver a more comprehensive visualization of the coronary artery. This ultrasonic-only intravascular imaging technique, based on a moderate refinement of the conventional IVUS system, is not only cost-effective from the perspective of manufacturing and clinical practice, but also holds the promise of future translation into clinical benefits. PMID:25585394

  19. Harmonic analysis of irradiation asymmetry for cylindrical implosions driven by high-frequency rotating ion beams.

    PubMed

    Bret, A; Piriz, A R; Tahir, N

    2012-03-01

    Cylindrical implosions driven by intense heavy ion beams should be instrumental in the near future for study of high-energy-density matter. By rotating the beam by means of a high-frequency wobbler, it should be possible to deposit energy in the outer layers of a cylinder, compressing the material deposited in its core. The beam's temporal profile should, however, generate an inevitable irradiation asymmetry likely to feed the Rayleigh-Taylor instability (RTI) during the implosion phase. In this paper, we compute the Fourier components of the target irradiation in order to make the connection with previous works on the RTI performed in this setting. Implementing one- and two-dimensional beam models, we find that these components can be expressed exactly in terms of the Fourier transform of the temporal beam profile. If T is the beam duration and Ω its rotation frequency, "magic products" ΩT can be identified which cancel the first harmonic of the deposited density, resulting in an improved irradiation symmetry. PMID:22587191

  20. Efficient Procedure for the Numerical Calculation of Harmonic Vibrational Frequencies Based on Internal Coordinates

    SciTech Connect

    Miliordos, Evangelos; Xantheas, Sotiris S.

    2013-08-15

    We propose a general procedure for the numerical calculation of the harmonic vibrational frequencies that is based on internal coordinates and Wilson’s GF methodology via double differentiation of the energy. The internal coordinates are defined as the geometrical parameters of a Z-matrix structure, thus avoiding issues related to their redundancy. Linear arrangements of atoms are described using a dummy atom of infinite mass. The procedure has been automated in FORTRAN90 and its main advantage lies in the nontrivial reduction of the number of single-point energy calculations needed for the construction of the Hessian matrix when compared to the corresponding number using double differentiation in Cartesian coordinates. For molecules of C1 symmetry the computational savings in the energy calculations amount to 36N – 30, where N is the number of atoms, with additional savings when symmetry is present. Typical applications for small and medium size molecules in their minimum and transition state geometries as well as hydrogen bonded clusters (water dimer and trimer) are presented. Finally, in all cases the frequencies based on internal coordinates differ on average by <1 cm–1 from those obtained from Cartesian coordinates.

  1. Imaging the bipolarity of myosin filaments with Interferometric Second Harmonic Generation microscopy

    PubMed Central

    Rivard, Maxime; Couture, Charles-André; Miri, Amir K.; Laliberté, Mathieu; Bertrand-Grenier, Antony; Mongeau, Luc; Légaré, François

    2013-01-01

    We report that combining interferometry with Second Harmonic Generation (SHG) microscopy provides valuable information about the relative orientation of noncentrosymmetric structures composing tissues. This is confirmed through the imaging of rat medial gastrocnemius muscle. The inteferometric Second Harmonic Generation (ISHG) images reveal that each side of the myosin filaments composing the A band of the sarcomere generates π phase shifted SHG signal which implies that the myosin proteins at each end of the filaments are oriented in opposite directions. This highlights the bipolar structural organization of the myosin filaments and shows that muscles can be considered as a periodically poled biological structure. PMID:24156065

  2. Fringe-free, background-free, collinear third-harmonic generation frequency-resolved optical gating measurements for multiphoton microscopy

    NASA Astrophysics Data System (ADS)

    Chadwick, Rebecca; Spahr, Erik; Squier, Jeff A.; Durfee, Charles G.; Walker, Barry C.; Fittinghoff, David N.

    2006-11-01

    A background-free, fringe-free form of frequency-resolved optical gating using the third-harmonic signal generated from a glass coverslip is used to characterize 100 fs pulses at the focus of a 0.65 NA objective.

  3. Calculation and analysis of the harmonic vibrational frequencies in molecules at extreme pressure: Methodology and diborane as a test case

    NASA Astrophysics Data System (ADS)

    Cammi, R.; Cappelli, C.; Mennucci, B.; Tomasi, J.

    2012-10-01

    We present a new quantum chemical method for the calculation of the equilibrium geometry and the harmonic vibrational frequencies of molecular systems in dense medium at high pressures (of the order of GPa). The new computational method, named PCM-XP, is based on the polarizable continuum model (PCM), amply used for the study of the solvent effects at standard condition of pressure, and it is accompanied by a new method of analysis for the interpretation of the mechanisms underpinning the effects of pressure on the molecular geometries and the harmonic vibrational frequencies. The PCM-XP has been applied at the density functional theory level to diborane as a molecular system under high pressure. The computed harmonic vibrational frequencies as a function of the pressure have shown a satisfactory agreement with the corresponding experimental results, and the parallel application of the method of analysis has reveled that the effects of the pressure on the equilibrium geometry can be interpreted in terms of direct effects on the electronic charge distribution of the molecular solutes, and that the effects on the harmonic vibrational frequencies can be described in terms of two physically distinct effects of the pressure (curvature and relaxation) on the potential energy for the motion of the nuclei.

  4. Adaptive contrast imaging: transmit frequency optimization

    NASA Astrophysics Data System (ADS)

    Ménigot, Sébastien; Novell, Anthony; Voicu, Iulian; Bouakaz, Ayache; Girault, Jean-Marc

    2010-01-01

    Introduction: Since the introduction of ultrasound (US) contrast imaging, the imaging systems use a fixed emitting frequency. However it is known that the insonified medium is time-varying and therefore an adapted time-varying excitation is expected. We suggest an adaptive imaging technique which selects the optimal transmit frequency that maximizes the acoustic contrast. Two algorithms have been proposed to find an US excitation for which the frequency was optimal with microbubbles. Methods and Materials: Simulations were carried out for encapsulated microbubbles of 2 microns by considering the modified Rayleigh-Plesset equation for 2 MHz transmit frequency and for various pressure levels (20 kPa up to 420kPa). In vitro experiments were carried out using a transducer operating at 2 MHz and using a programmable waveform generator. Contrast agent was then injected into a small container filled with water. Results and discussions: We show through simulations and in vitro experiments that our adaptive imaging technique gives: 1) in case of simulations, a gain of acoustic contrast which can reach 9 dB compared to the traditional technique without optimization and 2) for in vitro experiments, a gain which can reach 18 dB. There is a non negligible discrepancy between simulations and experiments. These differences are certainly due to the fact that our simulations do not take into account the diffraction and nonlinear propagation effects. Further optimizations are underway.

  5. Second harmonic generation imaging of fascia within thick tissue block

    NASA Astrophysics Data System (ADS)

    Pfeffer, Christian P.; Olsen, Bjorn R.; Légaré, François

    2007-06-01

    Comparing the SHG image formation for thin sections of tail tendon fascia and skeletal muscle fascia, we demonstrate that the forward (F) and backward (B) SHG images are vastly different. In addition, despite the different arrangement of the collagen Type I fibrillar architecture forming these two fascias, their ratios of forward over backward signal (F/B) are nearly equal. SHG images of thick tissue blocks of the fascia-muscle unit show only backward features, as opposed to SHG images of tissue blocks of the fascia-tendon unit. These images are an amalgamation of forward and backward features due to the backscattering of forward components within tendon. These forward features disappear when this tissue block is immersed in glycerol as backscattering is hereby suppressed.

  6. Contrast-enhanced harmonic endoscopic ultrasound imaging: Basic principles, present situation and future perspectives

    PubMed Central

    Alvarez-Sánchez, María-Victoria; Napoléon, Bertrand

    2014-01-01

    Over the last decade, the development of stabilised microbubble contrast agents and improvements in available ultrasonic equipment, such as harmonic imaging, have enabled us to display microbubble enhancements on a greyscale with optimal contrast and spatial resolution. Recent technological advances made contrast harmonic technology available for endoscopic ultrasound (EUS) for the first time in 2008. Thus, the evaluation of microcirculation is now feasible with EUS, prompting the evolution of contrast-enhanced EUS from vascular imaging to images of the perfused tissue. Although the relevant experience is still preliminary, several reports have highlighted contrast-enhanced harmonic EUS (CH-EUS) as a promising noninvasive method to visualise and characterise lesions and to differentiate benign from malignant focal lesions. Even if histology remains the gold standard, the combination of CH-EUS and EUS fine needle aspiration (EUS-FNA) can not only render EUS more accurate but may also assist physicians in making decisions when EUS-FNA is inconclusive, increasing the yield of EUS-FNA by guiding the puncture with simultaneous imaging of the vascularity. The development of CH-EUS has also opened up exciting possibilities in other research areas, including monitoring responses to anticancer chemotherapy or to ethanol-induced pancreatic tissue ablation, anticancer therapies based on ultrasound-triggered drug and gene delivery, and therapeutic adjuvants by contrast ultrasound-induced apoptosis. Contrast harmonic imaging is gaining popularity because of its efficacy, simplicity and non-invasive nature, and many expectations are currently resting on this technique. If its potential is confirmed in the near future, contrast harmonic imaging will become a standard practice in EUS. PMID:25400439

  7. Second-harmonic frequency-resolved optical gating covering two and a half optical octaves using a single spectrometer

    NASA Astrophysics Data System (ADS)

    Marceau, C.; Thomas, S.; Kassimi, Y.; Gingras, G.; Witzel, B.

    2015-05-01

    We report the measurement of laser pulse shapes covering the range 580-3250 nm using second-harmonic generation frequency-resolved optical gating equipped with a single inexpensive visible-NIR miniature spectrometer and a single pair of homemade broadband beam splitters. Our experimental scheme exploits frequency up-conversion by BBO crystals and appropriate corrections for dispersion, beam splitter filtering and phase-matching efficiency. The signal and idler waves from a commercial optical parametric amplifier pumped by a Ti:Sapphire laser (26 fs, 1 kHz) have been characterized as well as their second harmonic. The pulse shapes out of a commercial difference frequency generation module mixing signal and idler have also been measured up to 3250 nm. The resulting pulses range from 20 to 120 fs, and their chirp characteristics are also exposed. Our approach is demonstrated over most of the doubling crystal transparency range.

  8. A Spectral Finite Element Approach to Modeling Soft Solids Excited with High-Frequency Harmonic Loads

    PubMed Central

    Brigham, John C.; Aquino, Wilkins; Aguilo, Miguel A.; Diamessis, Peter J.

    2010-01-01

    An approach for efficient and accurate finite element analysis of harmonically excited soft solids using high-order spectral finite elements is presented and evaluated. The Helmholtz-type equations used to model such systems suffer from additional numerical error known as pollution when excitation frequency becomes high relative to stiffness (i.e. high wave number), which is the case, for example, for soft tissues subject to ultrasound excitations. The use of high-order polynomial elements allows for a reduction in this pollution error, but requires additional consideration to counteract Runge's phenomenon and/or poor linear system conditioning, which has led to the use of spectral element approaches. This work examines in detail the computational benefits and practical applicability of high-order spectral elements for such problems. The spectral elements examined are tensor product elements (i.e. quad or brick elements) of high-order Lagrangian polynomials with non-uniformly distributed Gauss-Lobatto-Legendre nodal points. A shear plane wave example is presented to show the dependence of the accuracy and computational expense of high-order elements on wave number. Then, a convergence study for a viscoelastic acoustic-structure interaction finite element model of an actual ultrasound driven vibroacoustic experiment is shown. The number of degrees of freedom required for a given accuracy level was found to consistently decrease with increasing element order. However, the computationally optimal element order was found to strongly depend on the wave number. PMID:21461402

  9. A Daily Oscillation in the Fundamental Frequency and Amplitude of Harmonic Syllables of Zebra Finch Song

    PubMed Central

    Wood, William E.; Osseward, Peter J.; Roseberry, Thomas K.; Perkel, David J.

    2013-01-01

    Complex motor skills are more difficult to perform at certain points in the day (for example, shortly after waking), but the daily trajectory of motor-skill error is more difficult to predict. By undertaking a quantitative analysis of the fundamental frequency (FF) and amplitude of hundreds of zebra finch syllables per animal per day, we find that zebra finch song follows a previously undescribed daily oscillation. The FF and amplitude of harmonic syllables rises across the morning, reaching a peak near mid-day, and then falls again in the late afternoon until sleep. This oscillation, although somewhat variable, is consistent across days and across animals and does not require serotonin, as animals with serotonergic lesions maintained daily oscillations. We hypothesize that this oscillation is driven by underlying physiological factors which could be shared with other taxa. Song production in zebra finches is a model system for studying complex learned behavior because of the ease of gathering comprehensive behavioral data and the tractability of the underlying neural circuitry. The daily oscillation that we describe promises to reveal new insights into how time of day affects the ability to accomplish a variety of complex learned motor skills. PMID:24312654

  10. Sum frequency and second harmonic generation from the surface of a liquid microjet

    SciTech Connect

    Smolentsev, Nikolay; Chen, Yixing; Roke, Sylvie; Jena, Kailash C.; Brown, Matthew A.

    2014-11-14

    The use of a liquid microjet as a possible source of interest for Second Harmonic Generation (SHG) and Sum Frequency Generation (SFG) spectroscopy is examined. We measured non-resonant SHG scattering patterns from the air/water interface of a microjet of pure water and observe a strong enhancement of the SHG signal for certain scattering angles. These enhancements can be explained by the optical properties and the shape of the liquid microjet. SFG experiments at the surface of a liquid microjet of ethanol in air show that it is also possible to measure the coherent vibrational SFG spectrum of the ethanol/air interface in this way. Our findings are useful for future far-UV or X-ray based nonlinear optical surface experiments on liquid jets. In addition, combined X-ray photoelectron spectroscopy and SHG/SFG measurements are feasible, which will be very useful in improving our understanding of the molecular foundations of electrostatic and chemical surface properties and phenomena.

  11. A Spectral Finite Element Approach to Modeling Soft Solids Excited with High-Frequency Harmonic Loads.

    PubMed

    Brigham, John C; Aquino, Wilkins; Aguilo, Miguel A; Diamessis, Peter J

    2011-01-15

    An approach for efficient and accurate finite element analysis of harmonically excited soft solids using high-order spectral finite elements is presented and evaluated. The Helmholtz-type equations used to model such systems suffer from additional numerical error known as pollution when excitation frequency becomes high relative to stiffness (i.e. high wave number), which is the case, for example, for soft tissues subject to ultrasound excitations. The use of high-order polynomial elements allows for a reduction in this pollution error, but requires additional consideration to counteract Runge's phenomenon and/or poor linear system conditioning, which has led to the use of spectral element approaches. This work examines in detail the computational benefits and practical applicability of high-order spectral elements for such problems. The spectral elements examined are tensor product elements (i.e. quad or brick elements) of high-order Lagrangian polynomials with non-uniformly distributed Gauss-Lobatto-Legendre nodal points. A shear plane wave example is presented to show the dependence of the accuracy and computational expense of high-order elements on wave number. Then, a convergence study for a viscoelastic acoustic-structure interaction finite element model of an actual ultrasound driven vibroacoustic experiment is shown. The number of degrees of freedom required for a given accuracy level was found to consistently decrease with increasing element order. However, the computationally optimal element order was found to strongly depend on the wave number. PMID:21461402

  12. Wearable Second Harmonic Generation Imaging: The Sarcomeric Bridge to the Clinic.

    PubMed

    Williams, Justin C; Campagnola, Paul J

    2015-12-16

    Imaging of sarcomere dynamics in vivo in patients has significant clinical importance, as the structure and function is altered in numerous pathologies. In this issue of Neuron, Schnitzer and coworkers (Sanchez et al., 2015) demonstrate this capability through a miniature, wearable Second Harmonic Generation microscope. PMID:26687213

  13. Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.

    PubMed Central

    Campagnola, Paul J; Millard, Andrew C; Terasaki, Mark; Hoppe, Pamela E; Malone, Christian J; Mohler, William A

    2002-01-01

    We find that several key endogenous protein structures give rise to intense second-harmonic generation (SHG)-nonabsorptive frequency doubling of an excitation laser line. Second-harmonic imaging microscopy (SHIM) on a laser-scanning system proves, therefore, to be a powerful and unique tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architecture. Unlike fluorescence, SHG suffers no inherent photobleaching or toxicity and does not require exogenous labels. Unlike polarization microscopy, SHIM provides intrinsic confocality and deep sectioning in complex tissues. In this study, we demonstrate the clarity of SHIM optical sectioning within unfixed, unstained thick specimens. SHIM and two-photon excited fluorescence (TPEF) were combined in a dual-mode nonlinear microscopy to elucidate the molecular sources of SHG in live cells and tissues. SHG arose not only from coiled-coil complexes within connective tissues and muscle thick filaments, but also from microtubule arrays within interphase and mitotic cells. Both polarization dependence and a local symmetry cancellation effect of SHG allowed the signal from species generating the second harmonic to be decoded, by ratiometric correlation with TPEF, to yield information on local structure below optical resolution. The physical origin of SHG within these tissues is addressed and is attributed to the laser interaction with dipolar protein structures that is enhanced by the intrinsic chirality of the protein helices. PMID:11751336

  14. Erratum: Sources of Image Degradation in Fundamental and Harmonic Ultrasound Imaging: A Nonlinear, Full-Wave, Simulation Study

    PubMed Central

    Pinton, Gianmarco F.; Trahey, Gregg E.; Dahl, Jeremy J.

    2015-01-01

    A full-wave equation that describes nonlinear propagation in a heterogeneous attenuating medium is solved numerically with finite differences in the time domain. This numerical method is used to simulate propagation of a diagnostic ultrasound pulse through a measured representation of the human abdomen with heterogeneities in speed of sound, attenuation, density, and nonlinearity. Conventional delay-and-sum beamforming is used to generate point spread functions (PSFs) that display the effects of these heterogeneities. For the particular imaging configuration that is modeled, these PSFs reveal that the primary source of degradation in fundamental imaging is due to reverberation from near-field structures. Compared with fundamental imaging, reverberation clutter in harmonic imaging is 27.1 dB lower. Simulated tissue with uniform velocity but unchanged impedance characteristics indicates that for harmonic imaging, the primary source of degradation is phase aberration. PMID:21693410

  15. Ultrabroadband chirped pulse second-harmonic spectroscopy: measuring the frequency-dependent second-order response of different metal films.

    PubMed

    Metzger, Bernd; Gui, Lili; Giessen, Harald

    2014-09-15

    We introduce a spectroscopic method for measuring the frequency-dependent second-order response using ultrabroadband strongly chirped laser pulses. The dispersion suppresses nonlinear frequency mixing, hence the second-order response of a material can be unambiguously retrieved. We demonstrate this method by measuring the frequency-dependent second-harmonic (SH) response of the metals gold, aluminium, silver, and copper in the wavelength range of about 900-1150 nm and compare the results to classical SH spectroscopy. The SH spectra indicate that interband transitions in the metals influence the overall nonlinear optical response. PMID:26466254

  16. In vivo polarization dependant Second and Third harmonic generation imaging of Caenorhabditis elegans pharyngeal muscles

    NASA Astrophysics Data System (ADS)

    Filippidis, G.; Troulinaki, K.; Fotakis, C.; Tavernarakis, N.

    2009-07-01

    In this study Second and Third harmonic generation (SHG-THG) imaging measurements were performed to the pharyngeal muscles of the nematode Caenorhabditis elegans, in vivo with linearly polarized laser beam. Complementary information about the anatomy of the pharynx and the morphology of the anterior part of the worm were extracted. THG signals proved to have no dependence on incident light polarization, while SHG images are highly sensitive to the changes of the incident linearly polarized light.

  17. High-contrast imaging of mycobacterium tuberculosis using third-harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Kim, Bo Ram; Lee, Eungjang; Park, Seung-Han

    2015-07-01

    Nonlinear optical microcopy has become an important tool in investigating biomaterials due to its various advantages such as label-free imaging capabilities. In particular, it has been shown that third-harmonic generation (THG) signals can be produced at interfaces between an aqueous medium (e.g. cytoplasm, interstitial fluid) and a mineralized lipidic surface. In this work, we have demonstrated that label-free high-contrast THG images of the mycobacterium tuberculosis can be obtained using THG microscopy.

  18. Second harmonic generation imaging of dermal collagen component in human keloid tissue

    NASA Astrophysics Data System (ADS)

    Yu, H. B.; Chen, S.; Zhu, X. Q.; Yang, H. Q.; Chen, J. X.

    2011-01-01

    In this paper, we report second harmonic generation (SHG) imaging of human keloid tissue. High resolution SHG images of collagen component were obtained in the superficial, medial and deep dermis of human keloid tissue, respectively. Our results show that this method has a capability to observe the structure of collagen component in human keloid tissue, which will help to better understand the formation process of human keloid scar at the molecular level.

  19. Third-harmonic generation imaging of three-dimensional microstructures fabricated by photopolymerization.

    PubMed

    Kunwar, Puskal; Toivonen, Juha; Kauranen, Martti; Bautista, Godofredo

    2016-05-01

    We demonstrate the capability of polarized third-harmonic generation (THG) for high contrast imaging of three-dimensional microstructures fabricated by photopolymerization. Using circular polarization of fundamental light, background-free optically-sectioned THG images were obtained from laser-written photopolymerized microstructures. The technique has great potential for simple and noninvasive characterization of photopolymerized devices, which typically show poor contrast in conventional light microscopy. PMID:27137551

  20. UHF Radar observations at HAARP with HF pump frequencies near electron gyro-harmonics and associated ionospheric effects

    NASA Astrophysics Data System (ADS)

    Watkins, Brenton; Fallen, Christopher; Secan, James

    Results for HF modification experiments at the HAARP facility in Alaska are presented for experiments with the HF pump frequency near third and fourth electron gyro-harmonics. A UHF diagnostic radar with range resolution of 600 m was used to determine time-dependent altitudes of scattering from plasma turbulence during heating experiments. Experiments were conducted with multiple HF frequencies stepped by 20 kHz above and below the gyro-harmonic values. During times of HF heating the HAARP facility has sufficient power to enhance large-scale ionospheric densities in the lower ionosphere (about 150-200 km altitude) and also in the topside ionosphere (above about 350 km). In the lower ionosphere, time-dependent decreases of the altitude of radar scatter result from electron density enhancements. The effects are substantially different even for relatively small frequency steps of 20 kHz. In all cases the time-varying altitude decrease of radar scatter stops about 5-10 km below the gyro-harmonic altitude that is frequency dependent; we infer that electron density enhancements stop at this altitude where the radar signals stop decreasing with altitude. Experiments with corresponding total electron content (TEC) data show that for HF interaction altitudes above about 170 km there is substantial topside electron density increases due to upward electron thermal conduction. For lower altitudes of HF interaction the majority of the thermal energy is transferred to the neutral gas and no significant topside density increases are observed. By selecting an appropriate HF frequency a little greater than the gyro-harmonic value we have demonstrated that the ionospheric response to HF heating is a self-oscillating mode where the HF interaction altitude moves up and down with a period of several minutes. If the interaction region is above about 170 km this also produces a continuously enhanced topside electron density and upward plasma flux. Experiments using an FM scan with the HF

  1. Frequency domain analysis of knock images

    NASA Astrophysics Data System (ADS)

    Qi, Yunliang; He, Xin; Wang, Zhi; Wang, Jianxin

    2014-12-01

    High speed imaging-based knock analysis has mainly focused on time domain information, e.g. the spark triggered flame speed, the time when end gas auto-ignition occurs and the end gas flame speed after auto-ignition. This study presents a frequency domain analysis on the knock images recorded using a high speed camera with direct photography in a rapid compression machine (RCM). To clearly visualize the pressure wave oscillation in the combustion chamber, the images were high-pass-filtered to extract the luminosity oscillation. The luminosity spectrum was then obtained by applying fast Fourier transform (FFT) to three basic colour components (red, green and blue) of the high-pass-filtered images. Compared to the pressure spectrum, the luminosity spectra better identify the resonant modes of pressure wave oscillation. More importantly, the resonant mode shapes can be clearly visualized by reconstructing the images based on the amplitudes of luminosity spectra at the corresponding resonant frequencies, which agree well with the analytical solutions for mode shapes of gas vibration in a cylindrical cavity.

  2. Long-term imaging of mouse embryos using adaptive harmonic generation microscopy

    PubMed Central

    Thayil, Anisha; Watanabe, Tomoko; Jesacher, Alexander; Wilson, Tony; Srinivas, Shankar; Booth, Martin

    2012-01-01

    We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5–5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, such as lipid droplets, nucleoli, and plasma membranes. The embryos are found to develop normally during one-day-long discontinuous HG imaging, permitting the observation of several dynamic events, such as morula compaction and blastocyst formation. PMID:21529087

  3. Suppression of tissue harmonics for pulse-inversion contrast imaging using time reversal

    NASA Astrophysics Data System (ADS)

    Couture, Olivier; Aubry, Jean-François; Montaldo, Gabriel; Tanter, Mickael; Fink, Mathias

    2008-10-01

    Pulse-inversion (PI) sequences are sensitive to the nonlinear echoes from microbubbles allowing an improvement in the blood-to-tissue contrast. However, at larger mechanical indices, this contrast is reduced by harmonics produced during nonlinear propagation. A method for tissue harmonics cancellation exploiting time reversal is experimentally implemented using a 128-channel 12-bit emitter receiver. The probe calibration is performed by acquiring the nonlinear echo of a wire in water. These distorted pulses are time-reversed, optimized and used for the PI imaging of a tissue phantom. Compared to normal (straight) pulses, the time-reversed distorted pulses reduced the tissue signal in PI by 11 dB. The second harmonic signals from microbubbles flowing in a wall-less vessel were unaffected by the correction. This technique can thus increase the blood-to-tissue contrast ratio while keeping the pressure and the number of pulses constant.

  4. Suppression of tissue harmonics for pulse-inversion contrast imaging using time reversal.

    PubMed

    Couture, Olivier; Aubry, Jean-François; Montaldo, Gabriel; Tanter, Mickael; Fink, Mathias

    2008-10-01

    Pulse-inversion (PI) sequences are sensitive to the nonlinear echoes from microbubbles allowing an improvement in the blood-to-tissue contrast. However, at larger mechanical indices, this contrast is reduced by harmonics produced during nonlinear propagation. A method for tissue harmonics cancellation exploiting time reversal is experimentally implemented using a 128-channel 12-bit emitter receiver. The probe calibration is performed by acquiring the nonlinear echo of a wire in water. These distorted pulses are time-reversed, optimized and used for the PI imaging of a tissue phantom. Compared to normal (straight) pulses, the time-reversed distorted pulses reduced the tissue signal in PI by 11 dB. The second harmonic signals from microbubbles flowing in a wall-less vessel were unaffected by the correction. This technique can thus increase the blood-to-tissue contrast ratio while keeping the pressure and the number of pulses constant. PMID:18765888

  5. Effect of pulse to pulse variation of divergence, pointing and amplitude of copper vapor laser radiations on their second harmonic and sum frequency conversion

    NASA Astrophysics Data System (ADS)

    Prakash, Om; Mahakud, Ramakanta; Nakhe, Shankar V.; Dixit, Sudhir K.

    2013-09-01

    This paper presents the effect of single pulse stability of divergence angle, beam pointing angle and amplitude of green and yellow radiation pulses of an unstable resonator copper vapor laser (CVL) oscillator in the sum frequency (SF) mixing and second harmonic (SH). The conversion efficiency of sum frequency generation was lower compared to second harmonic processes despite larger fundamental power being used in sum frequency experiments. However the net UV power obtained at the sum frequency was higher than both of the second harmonic UV frequencies. Lower sum frequency generation (SFG) conversion efficiency compared to second harmonic generation (SHG) of individual CVL radiation is attributed to difference in single pulse stability of beam pointing, divergence and amplitude fluctuation of both CVL radiations in addition to commonly known fact of spatio-temporal mis-match. At the same fundamental input power, higher SH conversion efficiency of yellow compared to green is attributed to its better single pulse stability of beam pointing and divergence.

  6. Revealing the nature of the QPO and its harmonic in GX 339-4 using frequency-resolved spectroscopy

    NASA Astrophysics Data System (ADS)

    Axelsson, Magnus; Done, Chris

    2016-05-01

    We use frequency-resolved spectroscopy to examine the energy spectra of the prominent low-frequency quasi-periodic oscillation (QPO) and its harmonic in GX 339-4. We track the evolution of these spectra as the source makes a transition from a bright low/hard to hard intermediate state. In the hard/intermediate states, the QPO and time-averaged spectra are similar and the harmonic is either undetected or similar to the QPO. By contrast, in the softer states, the harmonic is dramatically softer than the QPO spectrum and the time-averaged spectrum, and the QPO spectrum is dramatically harder than the time-averaged spectrum. Clearly, the existence of these very different spectral shaped components mean that the time-averaged spectra are complex, as also seen by the fact that the softer spectra cannot be well described by a disc, Comptonization and its reflection. We use the frequency-resolved spectra to better constrain the model components, and find that the data are consistent with a time-averaged spectrum which has an additional low-temperature, optically thick Comptonization component. The harmonic can be described by this additional component alone, while the QPO spectrum is similar to that of the hard Comptonization and its reflection. Neither QPO nor harmonic shows signs of the disc component even when it is strong in the time-averaged spectrum. This adds to the growing evidence for inhomogeneous Comptonization in black hole binaries. While the similarity between the harmonic and QPO spectra in the intermediate state can be produced from the angular dependence of Compton scattering in a single region, this cannot explain the dramatic differences seen in the soft state. Instead, we propose that the soft Compton region is located predominantly above the disc while the hard Compton is from the hotter inner flow. Our results therefore point to multiple possible mechanisms for producing harmonic features in the power spectrum. The dominant mechanism in a given

  7. Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy

    PubMed Central

    Carriles, Ramón; Schafer, Dawn N.; Sheetz, Kraig E.; Field, Jeffrey J.; Cisek, Richard; Barzda, Virginijus; Sylvester, Anne W.; Squier, Jeffrey A.

    2009-01-01

    We review the current state of multiphoton microscopy. In particular, the requirements and limitations associated with high-speed multiphoton imaging are considered. A description of the different scanning technologies such as line scan, multifoci approaches, multidepth microscopy, and novel detection techniques is given. The main nonlinear optical contrast mechanisms employed in microscopy are reviewed, namely, multiphoton excitation fluorescence, second harmonic generation, and third harmonic generation. Techniques for optimizing these nonlinear mechanisms through a careful measurement of the spatial and temporal characteristics of the focal volume are discussed, and a brief summary of photobleaching effects is provided. Finally, we consider three new applications of multiphoton microscopy: nonlinear imaging in microfluidics as applied to chemical analysis and the use of two-photon absorption and self-phase modulation as contrast mechanisms applied to imaging problems in the medical sciences. PMID:19725639

  8. Simultaneous stimulated Raman scattering and higher harmonic generation imaging for liver disease diagnosis without labeling

    NASA Astrophysics Data System (ADS)

    Lin, Jian; Wang, Zi; Zheng, Wei; Huang, Zhiwei

    2014-02-01

    Nonlinear optical microscopy (e.g., higher harmonic (second-/third- harmonic) generation (HHG), simulated Raman scattering (SRS)) has high diagnostic sensitivity and chemical specificity, making it a promising tool for label-free tissue and cell imaging. In this work, we report a development of a simultaneous SRS and HHG imaging technique for characterization of liver disease in a bile-duct-ligation rat-modal. HHG visualizes collagens formation and reveals the cell morphologic changes associated with liver fibrosis; whereas SRS identifies the distributions of hepatic fat cells formed in steatosis liver tissue. This work shows that the co-registration of SRS and HHG images can be an effective means for label-free diagnosis and characterization of liver steatosis/fibrosis at the cellular and molecular levels.

  9. Characterization of the cytotoxicity and imaging properties of second-harmonic nanoparticles

    NASA Astrophysics Data System (ADS)

    Hsieh, Chia-Lung; Grange, Rachel; Pu, Ye; Psaltis, Demetri

    2010-08-01

    We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal structures have high second-harmonic generation (SHG) efficiency and provide high contrast in a generally non-structured cell environment. Here, we use barium titanate (BaTiO3) nanoparticles with tetragonal crystal structure as imaging probes. Cytotoxicity tests performed on BaTiO3 nanoparticles with mammalian cells did not result in toxic effects. Specifically, we observed no change in the cell metabolism after 24 hours incubation of the cells with high concentration of BaTiO3 nanoparticles. We demonstrate two methods of cell labeling with BaTiO3 nanoparticles for imaging. One is non-specific labeling via endocytosis of the cells, which results in a great number of the nanoparticles randomly distributed inside the cells. The other is specific labeling via surface functionalization of the nanoparticles with antibodies, which enables us to label specific cell membrane proteins with the nanoparticles. SHG imaging is compatible to two-photon microscopy and the SHG signal from nanoparticles can be easily detected with a standard two-photon confocal microscope. Our work provides the opportunity for long-term, three-dimensional cell tracking with secondharmonic nanoparticles.

  10. Frequency-resolved optical gating with the use of second-harmonic generation

    SciTech Connect

    DeLong, K.W.; Trebino, R. ); Hunter, J.; White, W.E. )

    1994-11-01

    We discuss the use of second-harmonic generation (SHG) as the nonlinearity in the technique of frequency-resolved optical gating (FROG) for measuring the full intensity and phase evolution of an arbitrary ultrashort pulse. FROG that uses a third-order nonlinearity in the polarization-gate geometry has proved extremely successful, and the algorithm required for extraction of the intensity and the phase from the experimental data is quite robust. However, for pulse intensities less than [similar to] 1 MW, third-order nonlinearities generate insufficient signal strength, and therefore SHG FROG appears necessary. We discuss the theoretical, algorithmic, and experimental considerations of SHG FROG in detail. SHG FROG has an ambiguity in the direction of time, and its traces are somewhat unintuitive. Also, previously published algorithms are generally ineffective at extracting the intensity and the phase of an arbitrary laser pulse from the SHG FROG trace. We present an improved pulse-retrieval algorithm, based on the method of generalized projections, that is far superior to the previously published algorithms, although it is still not so robust as the polarization-gate algorithm. We discuss experimental sources of error such as pump depletion and group-velocity mismatch. We also present several experimental examples of pulses measured with SHG FROG and show that the derived intensities and phases are in agreement with more conventional diagnostic techniques, and we demonstrate the high-dynamic-range capability of SHG FROG. We conclude that, despite the above drawbacks, SHG FROG should be useful in measuring low-energy pulses.

  11. Fundamental analysis and ex vivo validation of thermal lesion mapping using harmonic motion imaging for focused ultrasound (HMIFU)

    NASA Astrophysics Data System (ADS)

    Hou, Gary Y.; Luo, Jianwen; Maleke, Caroline; Vappou, Jonathan; Marquet, Fabrice; Konofagou, Elisa E.

    2012-10-01

    Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a novel high-intensity focused ultrasound (HIFU) therapy monitoring method with feasibilities demonstrated in vitro, ex vivo and in vivo. Its principle is based on Amplitude-modulated (AM) - Harmonic Motion Imaging (HMI), an oscillatory radiation force used for imaging the tissue mechanical response during thermal ablation. In this study, a theoretical framework of HMIFU is presented, comprising a customized nonlinear wave propagation model, a finite-element (FE) analysis module, and an image-formation model. The objective of this study is to develop such a framework in order to 1) assess the fundamental performance of HMIFU in detecting HIFU lesions based on the change in tissue apparent elasticity, i.e., the increasing Young's modulus, and the HIFU lesion size with respect to the HIFU exposure time and 2) validate the simulation findings ex vivo. The same HMI and HMIFU parameters as in the experimental studies were used, i.e., 4.5-MHz HIFU frequency and 25-Hz AM frequency. For a lesion-to-background Young's modulus ratio of 3, 6, and 9, the estimated HMI displacement ratios were equal to 1.65, 3.19, 4.59, respectively. In experiments, the HMI displacement followed a similar increasing trend of 1.19, 1.28, 1.78 at 10-s, 20-s, and 30-s HIFU exposure, respectively. In addition, moderate agreement in lesion size growth was also found in both simulations (16.2, 73.1 and 334.7 mm2) and experiments (26.2, 94.2 and 206.2 mm2). Therefore, the feasibility of HMIFU for HIFU lesion detection based on the underlying tissue elasticity changes was verified through the developed theoretical framework, i.e., validation of the fundamental performance of the HMIFU system for lesion detection, localization and quantification, was demonstrated both theoretically and ex vivo.

  12. Garner Valley Vibroseis Data Processing Using Time-Frequency Filtering Techniques to Remove Unwanted Harmonics and External Noise

    NASA Astrophysics Data System (ADS)

    Lord, N. E.; Wang, H. F.; Fratta, D.; Lancelle, C.; Chalari, A.

    2015-12-01

    Time-frequency filtering techniques can greatly improve data quality when combined with frequency swept seismic sources (vibroseis) recorded by seismic arrays by removing unwanted source harmonics or external noise sources (e.g., cultural or ambient noise). A source synchronous filter (SSF) is a time-frequency filter which only passes a specified width frequency band centered on the time varying frequency of the seismic source. A source delay filter (SDF) is a time-frequency filter which only passes those frequencies from the source within a specified delay time range. Both of these time-frequency filters operate on the uncorrelated vibroseis data and allow separate analysis of the source fundamental frequency and each harmonic. In either technique, the time-frequency function of the source can be captured from the source encoder or specified using two or more time-frequency points. SSF and SDF were both used in the processing of the vibroseis data collected in the September 2013 seismic experiment conducted at the NEES@UCSB Garner Valley field site. Three vibroseis sources were used: a 45 kN shear shaker, a 450 N portable mass shaker, and a 26 kN vibroseis truck. Seismic signals from these sources were recorded by two lines of 1 and 3 component accelerometers and geophones, and the Silixa Ltd's intelligent Distributed Acoustic Sensing (iDASTM ) system connected to 762 m of trenched fiber optical cable in a larger rectangular area. SSF and SDF improved vibroseis data quality, simplified data interpretation, and allowed new analysis techniques. This research is part of the larger DOE's PoroTomo project (URL: http://geoscience.wisc.edu/feigl/porotomo).

  13. Measuring strain using digital image correlation of second harmonic generation images.

    PubMed

    Wentzell, Scott; Sterling Nesbitt, Robert; Macione, James; Kotha, Shiva

    2013-08-01

    The micromechanical environment of bone is crucial to understanding both bone fracture and mechanobiological responses of osteocytes, yet few techniques exist that are capable of measuring strains on the micrometer scale. A method for measuring micrometer level strains has been developed based on digital image correlation (DIC) of second harmonic generation microscopy (SHGM) images. Bovine tibias milled into thin sections were imaged using SHGM under loads of 0 and 15 MPa. Strains were measured using DIC and compared to applied strain values. First and second principal strains decreased in magnitude as the analysis region area increased from 1750 µm(2) to 60,920 µm(2), converging to 1.23 ± 0.74 and -0.745 ± 0.9816 times the applied strain respectively. A representative sample histogram revealed regions of pure tensile and compressive strain, and that strains were highly heterogeneous ranging from 8410 to -8840 microstrain for an applied 2870 microstrain. Comparison with applied strain measures suggested that analysis sizes of 1750 µm(2) and greater were measuring strains on the tissue scale, and higher resolution is required for collagen fibrillar strains. Regions of low SHGM intensity ("dark" regions) were seen which are believed to be lacunar and perilacunar regions of low collagen density. However, no significant differences in strain magnitude were present in dark regions versus regions of high signal intensity. The proposed technique is effective for strains on the size order of bone microarchitecture, and would be useful for studies into the mechanical microenvironment during loading. The technique also has potential for in vivo studies in small animal models. PMID:23845730

  14. Spectral imaging of breast fibroadenoma using second-harmonic generation

    NASA Astrophysics Data System (ADS)

    Zheng, Liqin; Wang, Yuhua

    2014-09-01

    Fibroadenoma (FA), typically composed of stroma and epithelial cells, is a very common benign breast disease. Women with FA are associated with an increased risk of future breast cancer. The objective of this study was to demonstrate the potential of multiphoton laser scanning microscopy (MPLSM) for characterizing the morphology of collagen in the human breast fibroadenomas. In the study, high-contrast SHG images of human normal breast tissues and fibroadenoma tissues were obtained for comparison. The morphology of collagen was different between normal breast tissue and fibroadenoma. This study shows that MPLSM has the ability to distinguish fibroadenoma tissues from the normal breast tissues based on the noninvasive SHG imaging. With the advent of the clinical portability of miniature MPLSM, we believe that the technique has great potential to be used in vivo studies and for monitoring the treatment responses of fibroadenomas in clinical.

  15. A mechanism for plasma waves at the harmonics of the plasma frequency in the electron foreshock boundary

    NASA Technical Reports Server (NTRS)

    Klimas, A. J.

    1983-01-01

    A bump-on-tail unstable reduced velocity distribution, constructed from data obtained at the upstream boundary of the electron foreshock by the GSFC electron spectrometer experiment on the ISEE-1 satellite, is used as the initial plasma state for a numerical integration of the 1D-Vlasov-Maxwell system of equations. The integration is carried through the growth of the instability, beyond its saturation, and well into the stabilized plasma regime. A power spectrum computed for the electric field of the stabilized plasma is dominated by a narrow peak at the Bohm-Gross frequency of the unstable field mode but also contains significant power at the harmonics of the Bohm-Gross frequency. The harmonic power is in sharp peaks which are split into closely spaced doublets. The fundamental peak at the Bohm-Gross frequency is split into a closely spaced triplet. The mechanism for excitation of the second harmonic is shown to be second order wave-wave coupling. Previously announced in STAR as N83-17315

  16. Multi-line transmission in medical imaging using the second-harmonic signal.

    PubMed

    Prieur, Fabrice; Dénarié, Bastien; Austeng, Andreas; Torp, Hans

    2013-12-01

    The emergence of three-dimensional imaging in the field of medical ultrasound imaging has greatly increased the number of transmissions needed to insonify a whole volume. With a large number of transmissions comes a low image frame rate. When using classical transmission techniques, as in two-dimensional imaging, the frame rate becomes unacceptably low, prompting the use of alternative transmission patterns that require less time. One alternative is to use a multi-line transmission (MLT) technique which consists of transmitting several pulses simultaneously in different directions. Perturbations appear when acquiring and beamforming the signal in the direction of one pulse because of the pulses sent in other directions. The edge waves from the pulses transmitted in a different direction add to the signal transmitted in the direction of interest, resulting in artifacts in the final image. Taking advantage of the nonlinear propagation of sound in tissue, the second-harmonic signal can be used with the MLT technique. The image obtained using the second-harmonic signal, compared with an image obtained using the fundamental signal, should have reduced artifacts coming from other pulses transmitted simultaneously. Simulations, backed up by experiments imaging a wire target and an in vivo left ventricle, confirm that the hypothesis is valid. In the studied case, the perturbations appear as an increase in the signal level around the main echo of a point scatterer. When using the fundamental signal, the measured amplitude level of the perturbations was approximately -40 dB compared with the maximum signal amplitude (-27 dB in vivo), whereas it was around -60 dB (-45 dB in vivo) for the second-harmonic signal. The MLT technique encounters limitations in the very near field where the pulses overlap and the perturbation level also increases for images with strong speckle and low contrast. PMID:24297034

  17. Third harmonic generation imaging for fast, label-free pathology of human brain tumors

    PubMed Central

    Kuzmin, N. V.; Wesseling, P.; Hamer, P. C. de Witt; Noske, D. P.; Galgano, G. D.; Mansvelder, H. D.; Baayen, J. C.; Groot, M. L.

    2016-01-01

    In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment of tumor boundaries by the neurosurgeon is difficult. Therefore, there is an urgent need for tools that provide the neurosurgeon with pathological information during the operation. We show that third harmonic generation (THG) microscopy provides label-free, real-time images of histopathological quality; increased cellularity, nuclear pleomorphism, and rarefaction of neuropil in fresh, unstained human brain tissue could be clearly recognized. We further demonstrate THG images taken with a GRIN objective, as a step toward in situ THG microendoscopy of tumor boundaries. THG imaging is thus a promising tool for optical biopsies. PMID:27231629

  18. Label-free three-dimensional imaging of cell nucleus using third-harmonic generation microscopy

    SciTech Connect

    Lin, Jian; Zheng, Wei; Wang, Zi; Huang, Zhiwei

    2014-09-08

    We report the implementation of the combined third-harmonic generation (THG) and two-photon excited fluorescence (TPEF) microscopy for label-free three-dimensional (3-D) imaging of cell nucleus morphological changes in liver tissue. THG imaging shows regular spherical shapes of normal hepatocytes nuclei with inner chromatin structures while revealing the condensation of chromatins and nuclear fragmentations in hepatocytes of diseased liver tissue. Colocalized THG and TPEF imaging provides complementary information of cell nuclei and cytoplasm in tissue. This work suggests that 3-D THG microscopy has the potential for quantitative analysis of nuclear morphology in cells at a submicron-resolution without the need for DNA staining.

  19. Label-free three-dimensional imaging of cell nucleus using third-harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Lin, Jian; Zheng, Wei; Wang, Zi; Huang, Zhiwei

    2014-09-01

    We report the implementation of the combined third-harmonic generation (THG) and two-photon excited fluorescence (TPEF) microscopy for label-free three-dimensional (3-D) imaging of cell nucleus morphological changes in liver tissue. THG imaging shows regular spherical shapes of normal hepatocytes nuclei with inner chromatin structures while revealing the condensation of chromatins and nuclear fragmentations in hepatocytes of diseased liver tissue. Colocalized THG and TPEF imaging provides complementary information of cell nuclei and cytoplasm in tissue. This work suggests that 3-D THG microscopy has the potential for quantitative analysis of nuclear morphology in cells at a submicron-resolution without the need for DNA staining.

  20. A high frequency electromagnetic impedance imaging system

    SciTech Connect

    Tseng, Hung-Wen; Lee, Ki Ha; Becker, Alex

    2003-01-15

    Non-invasive, high resolution geophysical mapping of the shallow subsurface is necessary for delineation of buried hazardous wastes, detecting unexploded ordinance, verifying and monitoring of containment or moisture contents, and other environmental applications. Electromagnetic (EM) techniques can be used for this purpose since electrical conductivity and dielectric permittivity are representative of the subsurface media. Measurements in the EM frequency band between 1 and 100 MHz are very important for such applications, because the induction number of many targets is small and the ability to determine the subsurface distribution of both electrical properties is required. Earlier workers were successful in developing systems for detecting anomalous areas, but quantitative interpretation of the data was difficult. Accurate measurements are necessary, but difficult to achieve for high-resolution imaging of the subsurface. We are developing a broadband non-invasive method for accurately mapping the electrical conductivity and dielectric permittivity of the shallow subsurface using an EM impedance approach similar to the MT exploration technique. Electric and magnetic sensors were tested to ensure that stray EM scattering is minimized and the quality of the data collected with the high-frequency impedance (HFI) system is good enough to allow high-resolution, multi-dimensional imaging of hidden targets. Additional efforts are being made to modify and further develop existing sensors and transmitters to improve the imaging capability and data acquisition efficiency.

  1. Efficient 2(nd) and 4(th) harmonic generation of a single-frequency, continuous-wave fiber amplifier.

    PubMed

    Sudmeyer, Thomas; Imai, Yutaka; Masuda, Hisashi; Eguchi, Naoya; Saito, Masaki; Kubota, Shigeo

    2008-02-01

    We demonstrate efficient cavity-enhanced second and fourth harmonic generation of an air-cooled, continuous-wave (cw), single-frequency 1064 nm fiber-amplifier system. The second harmonic generator achieves up to 88% total external conversion efficiency, generating more than 20-W power at 532 nm wavelength in a diffraction-limited beam (M(2) < 1.05). The nonlinear medium is a critically phase-matched, 20-mm long, anti-reflection (AR) coated LBO crystal operated at 25 degrees C. The fourth harmonic generator is based on an AR-coated, Czochralski-grown beta-BaB(2)O(4) (BBO) crystal optimized for low loss and high damage threshold. Up to 12.2 W of 266-nm deep-UV (DUV) output is obtained using a 6-mm long critically phase-matched BBO operated at 40 degrees C. This power level is more than two times higher than previously reported for cw 266-nm generation. The total external conversion efficiency from the fundamental at 1064 nm to the fourth harmonic at 266 nm is >50%. PMID:18542230

  2. Imaging 2-D Structures With Receiver Functions Using Harmonic Stripping

    NASA Astrophysics Data System (ADS)

    Schulte-Pelkum, V.

    2010-12-01

    I present a novel technique to image dipping and anisotropic structures using receiver functions. Receiver functions isolate phase conversions from interfaces close to the seismic station. Standard analysis assumes a quasi-flat layered structure and dampens arrivals from dipping interfaces and anisotropic layers, with attempts to extract information on such structures relying on cumbersome and nonunique forward modeling. I use a simple relationship between the radial and transverse component receiver function to detect dipping and anisotropic layers and map their depth and orientation. For dipping interfaces, layers with horizontal or plunging axis anisotropy, and point scatterers, the following relationships hold: After subtracting the azimuthally invariant portion of the radial receiver functions, the remaining signal is an azimuthally shifted version of the transverse receiver functions. The strike of the dipping interface or anisotropy is given by the azimuth of polarity reversals, and the type of structure can be inferred from the amount of phase shift between the components. For a known structure type, the phase shift between the two components provides pseudoevents from back-azimuths with little seismicity. The technique allows structural mapping at depth akin to geological mapping of rock fabric and dipping layers at the surface. It reduces complex wavefield effects to two simple and geologically meaningful parameters, similar to shear wave splitting. I demonstrate the method on the Wind River Thrust as well as other structures within the Transportable Array footprint.

  3. Imaging Jupiter Radiation Belts At Low Frequencies

    NASA Astrophysics Data System (ADS)

    Girard, J. N.; de Pater, I.; Zarka, P.; Santos-Costa, D.; Sault, R.; Hess, S.; Cecconi, B.; Fender, R.; Pewg, Lofar

    2014-04-01

    The ultra-relativistic electrons, trapped in the inner radiation belts of Jupiter, generates a strong synchrotron radio emission (historically known as the jovian decimeter radiation (DIM)) which is beamed, polarized (~20% linear, ~1% circular) and broadband. It has been extensively observed by radio telescopes/ probes and imaged by radio interferometers over a wide frequency spectrum (from >300 MHz up to 22 GHz). This extended emission presents two main emission peaks constantly located on both sides of the planet close to the magnetic plane. High latitude emissions were also regularly observed at particular frequencies, times and in particular observational configurations. This region of the magnetosphere is "frozen" due to the strong magnetic field (~4.2 G as the equator) and therefore is forced to rotate at the planetary period (T≈9h55m). Due to the tilt (~ 10o) between the spin axis of the planet and the magnetic axis (which can be seen as dipolar in first approximation), the belts and the associated radio emission wobble around the planet center. The analysis of the flux at different frequencies highlighted spatial, temporal and spectral variabilities which origins are now partly understood. The emission varies at different time scales (short-time variations of hours to long-term variation over decades) due to the combination of visibility effect (wobbling, beaming, position of the observer in the magnetic rotating reference frame) [1], [2] and intrinsic local variations (interaction between relativistic electrons and satellites/dust, delayed effect of the solar wind ram pressure, impacts events) [3], [4], [5]. A complete framework is necessary to fully understand the source, loss and transport processes of the electrons originating from outside the belt, migrating by inward diffusion and populating the inner region of the magnetosphere. Only a few and unresolved measurements were made below 300 MHz and the nonsystematic observation of this radio emission

  4. Modeling of large aperture third harmonic frequency conversion of high power Nd:glass laser systems

    SciTech Connect

    Henesian, M.A.; Wegner, P.J.; Speck, D.R.; Bibeau, C.; Ehrlich, R.B.; Laumann, C.W.; Lawson, J.K.; Weiland, T.L.

    1991-03-13

    To provide high-energy, high-power beams at short wavelengths for inertial-confinement-fusion experiments, we routinely convert the 1.053-{mu}m output of the Nova, Nd:phosphate-glass, laser system to its third-harmonic wavelength. We describe performance and conversion efficiency modeling of the 3 {times} 3 arrays potassium-dihydrogen-phosphate crystal plates used for type II/type II phase-matched harmonic conversion of Nova 0.74-m diameter beams, and an alternate type I/type II phase-matching configuration that improves the third-harmonic conversion efficiency. These arrays provide energy conversion of up to 65% and intensity conversion to 70%. 19 refs., 11 figs.

  5. Spike-Mode Oscillation of a Single Frequency, Neodymium: YAG Ring Laser with Intracavity Second Harmonic Generation

    NASA Astrophysics Data System (ADS)

    Dixon, George Jefferies

    Spike-mode oscillation of a single-frequency, internally-doubled Nd:YAG laser under conditions of square -wave pump modulation is a potentially interesting technique for increasing the average harmonic conversion efficiency. To investigate this mode of operation, we have designed and built a unidirectional, Nd:YAG ring laser prototype which is capable of single-longitudinal mode oscillation at pump powers which are substantially above threshold. Initial study of this laser with diode-laser-array pumping yielded a maximum continuous-wave (cw) 1064-nm output power of 72 mW at an optical conversion efficiency exceeding 14%. Intracavity second harmonic generation was studied by inserting a crystal of potassium titanyl phosphate (KTP) inside the resonator and replacing the infrared output coupler with a mirror which was highly reflecting at 1064 nm and had high transmission at the 532-nm second harmonic. A maximum cw harmonic output power of 12 mW was observed from the laser at a pump power of 473 mW. Spike-mode oscillation could be achieved in the intracavity-doubled laser through square wave current modulation of the diode laser pump. Under optimal conditions, the average harmonic conversion efficiency was increased by over 100% under spiked conditions. Spike-mode oscillation with significant intracavity nonlinear coupling was observed to differ substantially from that of laser without the nonlinear crystal. The power-dependent harmonic output coupling had the effect of damping out relaxation oscillations and substantially limiting the peak spiked power. It was also observed to increase the amplitude and temporal stability of the spike pulse train and significantly increase the frequency range over which spiked oscillation would occur. A set of coupled rate equations relating the single -mode intracavity field to the gain in the laser medium was used to model the spike-mode oscillations of the intracavity -doubled ring. Numerical methods were used to obtain solutions

  6. Second harmonic and sum frequency generation on dye-coated surfaces using collinear and non-collinear excitation geometries. [Rhodamine 6G monolayers on glass

    SciTech Connect

    Muenchausen, R.E.; Nguyen, D.C.; Keller, R.A.; Nogar, N.S.

    1986-01-01

    Doubly resonantly enhanced sum frequency generation from rhodamine 6G monolayers adsorbed on glass substates is compared with resonantly enhanced second harmonic generation using a collinear excitation geometry. Second harmonic and sum frequency generation with a non-collinear excitation geometry is also reported where spatial filtering of the non-collinear output is shown to increase the scattered light rejection by more than 4 orders of magnitude.

  7. Second-harmonic generation imaging of collagen fibers in myocardium for atrial fibrillation diagnosis

    NASA Astrophysics Data System (ADS)

    Tsai, Ming-Rung; Chiu, Yu-Wei; Lo, Men Tzung; Sun, Chi-Kuang

    2010-03-01

    Atrial fibrillation (AF) is the most common irregular heart rhythm and the mortality rate for patients with AF is approximately twice the mortality rate for patients with normal sinus rhythm (NSR). Some research has indicated that myocardial fibrosis plays an important role in predisposing patients to AF. Therefore, realizing the relationship between myocardial collagen fibrosis and AF is significant. Second-harmonic generation (SHG) is an optically nonlinear coherent process to image the collagen network. We perform SHG microscopic imaging of the collagen fibers in the human atrial myocardium. Utilizing the SHG images, we can identify the differences in morphology and the arrangement of collagen fibers between NSR and AF tissues. We also quantify the arrangement of the collagen fibers using Fourier transform images and calculating the values of angle entropy. We indicate that SHG imaging, a nondestructive and reproducible method to analyze the arrangement of collagen fibers, can provide explicit information about the relationship between myocardial fibrosis and AF.

  8. Lensless Diffractive Imaging Using Tabletop Coherent High-Harmonic Soft-X-Ray Beams

    SciTech Connect

    Sandberg, Richard L.; Paul, Ariel; Raymondson, Daisy A.; Haedrich, Steffen; Gaudiosi, David M.; Holtsnider, Jim; Tobey, Ra'anan I.; Cohen, Oren; Murnane, Margaret M.; Kapteyn, Henry C.; Song, Changyong; Miao Jianwei; Liu Yanwei; Salmassi, Farhad

    2007-08-31

    We present the first experimental demonstration of lensless diffractive imaging using coherent soft x rays generated by a tabletop soft-x-ray source. A 29 nm high harmonic beam illuminates an object, and the subsequent diffraction is collected on an x-ray CCD camera. High dynamic range diffraction patterns are obtained by taking multiple exposures while blocking small-angle diffraction using beam blocks of varying size. These patterns reconstruct to images with 214 nm resolution. This work demonstrates a practical tabletop lensless microscope that promises to find applications in materials science, nanoscience, and biology.

  9. Chiral imaging of collagen by second-harmonic generation circular dichroism

    PubMed Central

    Lee, H.; Huttunen, M. J.; Hsu, K.-J.; Partanen, M.; Zhuo, G.-Y.; Kauranen, M.; Chu, S.-W.

    2013-01-01

    We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules. PMID:23761852

  10. Tripling the maximum imaging depth with third-harmonic generation microscopy.

    PubMed

    Yildirim, Murat; Durr, Nicholas; Ben-Yakar, Adela

    2015-09-01

    The growing interest in performing high-resolution, deep-tissue imaging has galvanized the use of longer excitation wavelengths and three-photon-based techniques in nonlinear imaging modalities. This study presents a threefold improvement in maximum imaging depth of ex vivo porcine vocal folds using third-harmonic generation (THG) microscopy at 1552-nm excitation wavelength compared to two-photon microscopy (TPM) at 776-nm excitation wavelength. The experimental, analytical, and Monte Carlo simulation results reveal that THG improves the maximum imaging depth observed in TPM significantly from 140 to 420 μm in a highly scattered medium, reaching the expected theoretical imaging depth of seven extinction lengths. This value almost doubles the previously reported normalized imaging depths of 3.5 to 4.5 extinction lengths using three-photon-based imaging modalities. Since tissue absorption is substantial at the excitation wavelength of 1552 nm, this study assesses the tissue thermal damage during imaging by obtaining the depth-resolved temperature distribution through a numerical simulation incorporating an experimentally obtained thermal relaxation time (τ). By shuttering the laser for a period of 2τ, the numerical algorithm estimates a maximum temperature increase of ∼2°C at the maximum imaging depth of 420 μm. The paper demonstrates that THG imaging using 1552 nm as an illumination wavelength with effective thermal management proves to be a powerful deep imaging modality for highly scattering and absorbing tissues, such as scarred vocal folds. PMID:26376941

  11. Tripling the maximum imaging depth with third-harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Yildirim, Murat; Durr, Nicholas; Ben-Yakar, Adela

    2015-09-01

    The growing interest in performing high-resolution, deep-tissue imaging has galvanized the use of longer excitation wavelengths and three-photon-based techniques in nonlinear imaging modalities. This study presents a threefold improvement in maximum imaging depth of ex vivo porcine vocal folds using third-harmonic generation (THG) microscopy at 1552-nm excitation wavelength compared to two-photon microscopy (TPM) at 776-nm excitation wavelength. The experimental, analytical, and Monte Carlo simulation results reveal that THG improves the maximum imaging depth observed in TPM significantly from 140 to 420 μm in a highly scattered medium, reaching the expected theoretical imaging depth of seven extinction lengths. This value almost doubles the previously reported normalized imaging depths of 3.5 to 4.5 extinction lengths using three-photon-based imaging modalities. Since tissue absorption is substantial at the excitation wavelength of 1552 nm, this study assesses the tissue thermal damage during imaging by obtaining the depth-resolved temperature distribution through a numerical simulation incorporating an experimentally obtained thermal relaxation time (τ). By shuttering the laser for a period of 2τ, the numerical algorithm estimates a maximum temperature increase of ˜2°C at the maximum imaging depth of 420 μm. The paper demonstrates that THG imaging using 1552 nm as an illumination wavelength with effective thermal management proves to be a powerful deep imaging modality for highly scattering and absorbing tissues, such as scarred vocal folds.

  12. Texture analysis applied to second harmonic generation image data for ovarian cancer classification.

    PubMed

    Wen, Bruce L; Brewer, Molly A; Nadiarnykh, Oleg; Hocker, James; Singh, Vikas; Mackie, Thomas R; Campagnola, Paul J

    2014-09-01

    Remodeling of the extracellular matrix has been implicated in ovarian cancer. To quantitate the remodeling, we implement a form of texture analysis to delineate the collagen fibrillar morphology observed in second harmonic generation microscopy images of human normal and high grade malignant ovarian tissues. In the learning stage, a dictionary of “textons”—frequently occurring texture features that are identified by measuring the image response to a filter bank of various shapes, sizes, and orientations—is created. By calculating a representative model based on the texton distribution for each tissue type using a training set of respective second harmonic generation images, we then perform classification between images of normal and high grade malignant ovarian tissues. By optimizing the number of textons and nearest neighbors, we achieved classification accuracy up to 97% based on the area under receiver operating characteristic curves (true positives versus false positives). The local analysis algorithm is a more general method to probe rapidly changing fibrillar morphologies than global analyses such as FFT. It is also more versatile than other texture approaches as the filter bank can be highly tailored to specific applications (e.g., different disease states) by creating customized libraries based on common image features. PMID:26296156

  13. Texture analysis applied to second harmonic generation image data for ovarian cancer classification

    NASA Astrophysics Data System (ADS)

    Wen, Bruce L.; Brewer, Molly A.; Nadiarnykh, Oleg; Hocker, James; Singh, Vikas; Mackie, Thomas R.; Campagnola, Paul J.

    2014-09-01

    Remodeling of the extracellular matrix has been implicated in ovarian cancer. To quantitate the remodeling, we implement a form of texture analysis to delineate the collagen fibrillar morphology observed in second harmonic generation microscopy images of human normal and high grade malignant ovarian tissues. In the learning stage, a dictionary of "textons"-frequently occurring texture features that are identified by measuring the image response to a filter bank of various shapes, sizes, and orientations-is created. By calculating a representative model based on the texton distribution for each tissue type using a training set of respective second harmonic generation images, we then perform classification between images of normal and high grade malignant ovarian tissues. By optimizing the number of textons and nearest neighbors, we achieved classification accuracy up to 97% based on the area under receiver operating characteristic curves (true positives versus false positives). The local analysis algorithm is a more general method to probe rapidly changing fibrillar morphologies than global analyses such as FFT. It is also more versatile than other texture approaches as the filter bank can be highly tailored to specific applications (e.g., different disease states) by creating customized libraries based on common image features.

  14. Texture analysis applied to second harmonic generation image data for ovarian cancer classification

    PubMed Central

    Wen, Bruce L.; Brewer, Molly A.; Nadiarnykh, Oleg; Hocker, James; Singh, Vikas; Mackie, Thomas R.; Campagnola, Paul J.

    2014-01-01

    Abstract. Remodeling of the extracellular matrix has been implicated in ovarian cancer. To quantitate the remodeling, we implement a form of texture analysis to delineate the collagen fibrillar morphology observed in second harmonic generation microscopy images of human normal and high grade malignant ovarian tissues. In the learning stage, a dictionary of “textons”—frequently occurring texture features that are identified by measuring the image response to a filter bank of various shapes, sizes, and orientations—is created. By calculating a representative model based on the texton distribution for each tissue type using a training set of respective second harmonic generation images, we then perform classification between images of normal and high grade malignant ovarian tissues. By optimizing the number of textons and nearest neighbors, we achieved classification accuracy up to 97% based on the area under receiver operating characteristic curves (true positives versus false positives). The local analysis algorithm is a more general method to probe rapidly changing fibrillar morphologies than global analyses such as FFT. It is also more versatile than other texture approaches as the filter bank can be highly tailored to specific applications (e.g., different disease states) by creating customized libraries based on common image features. PMID:26296156

  15. Coherent diffractive imaging microscope with a high-order harmonic source.

    PubMed

    Dinh, Khuong Ba; Le, Hoang Vu; Hannaford, Peter; Dao, Lap Van

    2015-06-10

    We report the generation of highly coherent extreme ultraviolet sources with wavelengths around 30 and 10 nm by phase-matched high-order harmonic generation (HHG) in a gas cell filled with argon and helium, respectively. We then perform coherent diffractive imaging (CDI) by using a focused narrow-bandwidth HHG source with wavelength around 30 nm as an illumination beam for two kinds of samples. The first is a transmission sample and the second is a absorption sample. In addition, we report the successful reconstruction of a complex absorption sample using a tabletop high-harmonic source. This will open the path to the realization of a compact soft x-ray microscope to investigate biological samples such as membrane proteins. PMID:26192827

  16. Echelle spectrograph calibration with a frequency comb based on a harmonically mode-locked fiber laser: a proposal.

    PubMed

    McFerran, J J

    2009-05-10

    Details for constructing an astronomical frequency comb suitable as a wavelength reference for échelle spectrographs associated with optical telescopes are outlined. The source laser for the frequency comb is a harmonically mode-locked fiber laser with a central wavelength of 1.56 microm. The means of producing a repetition rate greater than 7 GHz and a peak optical power of approximately 8 kW are discussed. Conversion of the oscillator light into the visible can occur through a two-step process of (i) nonlinear conversion in periodically poled lithium niobate and (ii) spectral broadening in photonic crystal fiber. While not necessarily octave spanning in spectral range to permit the use of an f -to- 2f interferometer for offset frequency control, the frequency comb can be granted accuracy by linking the mode spacing and a comb tooth to separate frequency references. The design avoids the use of a Fabry-Perot cavity to increase the mode spacing of the frequency comb; however, the level of supermode suppression and sideband asymmetry in the fiber oscillator and in the subsequent frequency conversion stages are aspects that need to be experimentally tested. PMID:19424399

  17. Echelle spectrograph calibration with a frequency comb based on a harmonically mode-locked fiber laser: a proposal

    SciTech Connect

    McFerran, J. J.

    2009-05-10

    Details for constructing an astronomical frequency comb suitable as a wavelength reference for echelle spectrographs associated with optical telescopes are outlined. The source laser for the frequency comb is a harmonically mode-locked fiber laser with a central wavelength of 1.56 {mu}m. The means of producing a repetition rate greater than 7 GHz and a peak optical power of {approx}8 kW are discussed. Conversion of the oscillator light into the visible can occur through a two-step process of (i) nonlinear conversion in periodically poled lithium niobate and (ii) spectral broadening in photonic crystal fiber. While not necessarily octave spanning in spectral range to permit the use of an f -to- 2f interferometer for offset frequency control, the frequency comb can be granted accuracy by linking the mode spacing and a comb tooth to separate frequency references. The design avoids the use of a Fabry-Perot cavity to increase the mode spacing of the frequency comb; however, the level of supermode suppression and sideband asymmetry in the fiber oscillator and in the subsequent frequency conversion stages are aspects that need to be experimentally tested.

  18. Image enhancement by non-linear extrapolation in frequency space

    NASA Technical Reports Server (NTRS)

    Anderson, Charles H. (Inventor); Greenspan, Hayit K. (Inventor)

    1998-01-01

    An input image is enhanced to include spatial frequency components having frequencies higher than those in an input image. To this end, an edge map is generated from the input image using a high band pass filtering technique. An enhancing map is subsequently generated from the edge map, with the enhanced map having spatial frequencies exceeding an initial maximum spatial frequency of the input image. The enhanced map is generated by applying a non-linear operator to the edge map in a manner which preserves the phase transitions of the edges of the input image. The enhanced map is added to the input image to achieve a resulting image having spatial frequencies greater than those in the input image. Simplicity of computations and ease of implementation allow for image sharpening after enlargement and for real-time applications such as videophones, advanced definition television, zooming, and restoration of old motion pictures.

  19. Enabling Multiphoton and Second Harmonic Generation Imaging in Paraffin-Embedded and Histologically Stained Sections

    PubMed Central

    Monaghan, Michael G.; Kroll, Sebastian; Brucker, Sara Y.

    2016-01-01

    Nonlinear microscopy, namely multiphoton imaging and second harmonic generation (SHG), is an established noninvasive technique useful for the imaging of extracellular matrix (ECM). Typically, measurements are performed in vivo on freshly excised tissues or biopsies. In this article, we describe the effect of rehydrating paraffin-embedded sections on multiphoton and SHG emission signals and the acquisition of nonlinear images from hematoxylin and eosin (H&E)-stained sections before and after a destaining protocol. Our results reveal that bringing tissue sections to a physiological state yields a significant improvement in nonlinear signals, particularly in SHG. Additionally, the destaining of sections previously processed with H&E staining significantly improves their SHG emission signals during imaging, thereby allowing sufficient analysis of collagen in these sections. These results are important for researchers and pathologists to obtain additional information from paraffin-embedded tissues and archived samples to perform retrospective analysis of the ECM or gain additional information from rare samples. PMID:27018844

  20. Imaging of targeted lipid microbubbles to detect cancer cells using third harmonic generation microscopy

    PubMed Central

    Harpel, Kaitlin; Baker, Robert Dawson; Amirsolaimani, Babak; Mehravar, Soroush; Vagner, Josef; Matsunaga, Terry O.; Banerjee, Bhaskar; Kieu, Khanh

    2016-01-01

    The use of receptor-targeted lipid microbubbles imaged by ultrasound is an innovative method of detecting and localizing disease. However, since ultrasound requires a medium between the transducer and the object being imaged, it is impractical to apply to an exposed surface in a surgical setting where sterile fields need be maintained and ultrasound gel may cause the bubbles to collapse. Multiphoton microscopy (MPM) is an emerging tool for accurate, label-free imaging of tissues and cells with high resolution and contrast. We have recently determined a novel application of MPM to be used for detecting targeted microbubble adherence to the upregulated plectin-receptor on pancreatic tumor cells. Specifically, the third-harmonic generation response can be used to detect bound microbubbles to various cell types presenting MPM as an alternative and useful imaging method. This is an interesting technique that can potentially be translated as a diagnostic tool for the early detection of cancer and inflammatory disorders. PMID:27446711

  1. Imaging of targeted lipid microbubbles to detect cancer cells using third harmonic generation microscopy.

    PubMed

    Harpel, Kaitlin; Baker, Robert Dawson; Amirsolaimani, Babak; Mehravar, Soroush; Vagner, Josef; Matsunaga, Terry O; Banerjee, Bhaskar; Kieu, Khanh

    2016-07-01

    The use of receptor-targeted lipid microbubbles imaged by ultrasound is an innovative method of detecting and localizing disease. However, since ultrasound requires a medium between the transducer and the object being imaged, it is impractical to apply to an exposed surface in a surgical setting where sterile fields need be maintained and ultrasound gel may cause the bubbles to collapse. Multiphoton microscopy (MPM) is an emerging tool for accurate, label-free imaging of tissues and cells with high resolution and contrast. We have recently determined a novel application of MPM to be used for detecting targeted microbubble adherence to the upregulated plectin-receptor on pancreatic tumor cells. Specifically, the third-harmonic generation response can be used to detect bound microbubbles to various cell types presenting MPM as an alternative and useful imaging method. This is an interesting technique that can potentially be translated as a diagnostic tool for the early detection of cancer and inflammatory disorders. PMID:27446711

  2. Frequency-Domain Approach To Determine Magnetic Address-Sensor Separation Distance Using the Harmonic Ratio Method.

    PubMed

    Young, Colin C; Blackley, Benjamin W; Porter, Marc D; Granger, Michael C

    2016-02-16

    In this work, we describe an approach to determine the distance separating a magnetic address from a scanning magnetoresistive sensor, a critical adjustable parameter for certain bioassay analyses where magnetic nanoparticles are used as labels. Our approach is leveraged from the harmonic ratio method (HRM), a method used in the hard drive industry to control the distance separating a magnetoresistive read head from its data platter with nanometer resolution. At the heart of the HRM is an amplitude comparison of a signal's fundamental frequency to that of its harmonics. When the signal is derived from the magnetic field pattern of a periodic array of magnetic addresses, the harmonic ratio contains the information necessary to determine the separation between the address array and the read head. The elegance of the HRM is that there is no need of additional components to the detection platform to determine a separation distance; the streaming "bit signal" contains all the information needed. In this work, we demonstrate that the tenets governing HRM used in the hard drive industry can be applied to the bioanalytical arena where submicrometer to 100 μm separations are required. PMID:26879366

  3. Sources of Image Degradation in Fundamental and Harmonic Ultrasound Imaging: A Nonlinear, Full-Wave, Simulation Study

    PubMed Central

    Pinton, Gianmarco F.; Trahey, Gregg E.; Dahl, Jeremy J.

    2011-01-01

    A full-wave equation that describes nonlinear propagation in a heterogeneous attenuating medium is solved numerically with finite differences in the time domain (FDTD). This numerical method is used to simulate propagation of a diagnostic ultrasound pulse through a measured representation of the human abdomen with heterogeneities in speed of sound, attenuation, density, and nonlinearity. Conventional delay-and-sum beamforming is used to generate point spread functions (PSF) that display the effects of these heterogeneities. For the particular imaging configuration that is modeled, these PSFs reveal that the primary source of degradation in fundamental imaging is due to reverberation from near-field structures. Compared to fundamental imaging, reverberation clutter in harmonic imaging is 27.1 dB lower. Simulated tissue with uniform velocity but unchanged impedance characteristics indicates that for fundamental imaging, the primary source of degradation is phase aberration. PMID:21507753

  4. Investigation of the effect of subcutaneous fat on image quality performance of 2D conventional imaging and tissue harmonic imaging.

    PubMed

    Browne, Jacinta E; Watson, Amanda J; Hoskins, Peter R; Elliott, Alex T

    2005-07-01

    Tissue harmonic imaging (THI) has been reported to improve contrast resolution, tissue differentiation and overall image quality in clinical examinations. However, a study carried out previously by the authors (Brown et al. 2004) found improvements only in spatial resolution and not in contrast resolution or anechoic target detection. This result may have been due to the homogeneity of the phantom. Biologic tissues are generally inhomogeneous and THI has been reported to improve image quality in the presence of large amounts of subcutaneous fat. The aims of the study were to simulate the distortion caused by subcutaneous fat to image quality and thus investigate further the improvements reported in anechoic target detection and contrast resolution performance with THI compared with 2D conventional imaging. In addition, the effect of three different types of fat-mimicking layer on image quality was examined. The abdominal transducer of two ultrasound scanners with 2D conventional imaging and THI were tested, the 4C1 (Aspen-Acuson, Siemens Co., CA, USA) and the C5-2 (ATL HDI 5000, ATL/Philips, Amsterdam, The Netherlands). An ex vivo subcutaneous pig fat layer was used to replicate beam distortion and phase aberration seen clinically in the presence of subcutaneous fat. Three different types of fat-mimicking layers (olive oil, lard and lard with fish oil capsules) were evaluated. The subcutaneous pig fat layer demonstrated an improvement in anechoic target detection with THI compared with 2D conventional imaging, but no improvement was demonstrated in contrast resolution performance; a similar result was found in a previous study conducted by this research group (Brown et al. 2004) while using this tissue-mimicking phantom without a fat layer. Similarly, while using the layers of olive oil, lard and lard with fish oil capsules, improvements due to THI were found in anechoic target detection but, again, no improvements were found for contrast resolution for any of the

  5. In vivo Application of Short-lag Spatial Coherence and Harmonic Spatial Coherence Imaging in Fetal Ultrasound

    PubMed Central

    Kakkad, Vaibhav; Dahl, Jeremy; Ellestad, Sarah; Trahey, Gregg

    2014-01-01

    Fetal scanning is one of the most common applications of ultrasound imaging and serves as a source of vital information about maternal and fetal health. Visualization of clinically relevant structures, however, can be severely compromised in difficult-to-image patients due to poor resolution and the presence of high levels of acoustical noise or clutter. We have developed novel coherence-based beamforming methods called Short-Lag Spatial Coherence (SLSC) imaging and Harmonic Spatial Coherence imaging (HSCI) and applied them to suppress the effects of clutter in fetal imaging. This method is used to create images of the spatial coherence of the backscattered ultrasound as opposed to images of echo magnitude. We present the results of a patient study to assess the benefits of coherence-based beamforming in the context of first trimester fetal exams. Matched fundamental B-mode, SLSC, harmonic B-mode and HSCI images were generated using raw RF data collected on 11 volunteers in the first trimester of pregnancy. The images were compared for qualitative differences in image texture and target conspicuity as well as using quantitative imaging metrics such as SNR, CNR and contrast. SLSC and HSCI showed statistically significant improvements across all imaging metrics compared to B-mode and harmonic B-mode respectively. These improvements were greatest for poor quality B-mode images where contrast of anechoic targets was improved from 15 dB in fundamental B-mode to 27 dB in SLSC and 17 dB in harmonic B-mode to 30 dB in HSCI. CNR improved from 1.4 to 2.5 in the fundamental images and 1.4 to 3.1 in the harmonic case. These results exhibit the potential of coherence-based beamforming to improve image quality and target detectability, especially in high noise environments. PMID:25116292

  6. Texture analysis applied to second harmonic generation image data for disease classification and development of a multi-view second harmonic generation imaging platform

    NASA Astrophysics Data System (ADS)

    Wen, Lianggong

    Many diseases, e.g. ovarian cancer, breast cancer and pulmonary fibrosis, are commonly associated with drastic alterations in surrounding connective tissue, and changes in the extracellular matrix (ECM) are associated with the vast majority of cellular processes in disease progression and carcinogenesis: cell differentiation, proliferation, biosynthetic ability, polarity, and motility. We use second harmonic generation (SHG) microscopy for imaging the ECM because it is a non-invasive, non-linear laser scanning technique with high sensitivity and specificity for visualizing fibrillar collagen. In this thesis, we are interested in developing imaging techniques to understand how the ECM, especially the collagen architecture, is remodeled in diseases. To quantitate remodeling, we implement a 3D texture analysis to delineate the collagen fibrillar morphology observed in SHG microscopy images of human normal and high grade malignant ovarian tissues. In the learning stage, a dictionary of "textons"---frequently occurring texture features that are identified by measuring the image response to a filter bank of various shapes, sizes, and orientations---is created. By calculating a representative model based on the texton distribution for each tissue type using a training set of respective mages, we then perform classification between normal and high grade malignant ovarian tissues classification based on the area under receiver operating characteristic curves (true positives versus false positives). The local analysis algorithm is a more general method to probe rapidly changing fibrillar morphologies than global analyses such as FFT. It is also more versatile than other texture approaches as the filter bank can be highly tailored to specific applications (e.g., different disease states) by creating customized libraries based on common image features. Further, we describe the development of a multi-view 3D SHG imaging platform. Unlike fluorescence microscopy, SHG excites

  7. Evaluation of the developmental toxicity of 60 Hz magnetic fields and harmonic frequencies in Sprague-Dawley rats.

    PubMed

    Ryan, B M; Polen, M; Gauger, J R; Mallett, E; Kearns, M B; Bryan, T L; McCormick, D L

    2000-05-01

    Experimental data suggest that exposure to the 50 and 60 Hz sinusoidal components of power-frequency magnetic fields (MFs) does not have an adverse impact on fetal development. However, the possible developmental toxicity of MF harmonics has not been investigated. This study was designed to determine whether exposure to 180 Hz MFs (third harmonic), alone or in combination with 60 Hz MFs, induces birth defects in Sprague-Dawley rats. Groups of sperm-positive dams (> or =20/group) were exposed for 18.5 h per day from gestation days 6 through 19 to (1) ambient MFs only (<0.0001 mT; sham controls); (2) 60 Hz MFs at 0.2 mT; (3) 180 Hz MFs at 0.2 mT; or (4) 60 Hz + 180 Hz MFs (10% third harmonic; total field strength = 0.2 mT). Litter size, litter weight, percentage live births, sex ratio, and number of resorption sites were determined for each dam, and gross external, visceral, cephalic and skeletal examinations were performed on all fetuses. MF exposure had no significant effects on litter size, litter weight, or fetal development. With the exception of common rib variants, the incidence of fetal anomalies was comparable in all groups. A small increase in the incidence of rib variants was seen in the group exposed to 60 Hz + 180 Hz MFs; however, the incidence of rib variants in this group was similar to that in historical controls from our laboratory. These data extend the existing database on developmental toxicity of MFs by demonstrating that exposure to 180 Hz MFs, either alone or superimposed on an underlying 60 Hz signal, does not induce biologically significant developmental toxicity. These data do not support the hypothesis that exposure to power-frequency MFs is an important risk factor for fetal development. PMID:10790286

  8. Microwave Imaging Reflectometry for the study of Edge Harmonic Oscillations on DIII-D

    NASA Astrophysics Data System (ADS)

    Ren, X.; Chen, M.; Chen, X.; Domier, C. W.; Ferraro, N. M.; Kramer, G. J.; Luhmann, N. C., Jr.; Muscatello, C. M.; Nazikian, R.; Shi, L.; Tobias, B. J.; Valeo, E.

    2015-10-01

    Quiescent H-mode (QH-mode) is an ELM free mode of operation in which edge-localized harmonic oscillations (EHOs) are believed to enhance particle transport, thereby stabilizing ELMs and preventing damage to the divertor and plasma facing components. Microwave Imaging Reflectometer (MIR) enabling direct comparison between the measured and simulated 2D images of density fluctuations near the edge can determine the 2D structure of density oscillation, which can help to explain the physics behind EHO modes. MIR data sometimes indicate a counter-propagation between dominant (n=1) and higher harmonic modes of coherent EHOs in the steep gradient regions of the pedestal. To preclude diagnostic artifacts, we have performed forward modeling that includes possible optical mis-alignments to show that offsets between transmitting and receiving antennas do not account for this feature. We have also simulated the non-linear structure of the EHO modes, which induces multiple harmonics that are properly charaterized in the synthetic diagnostic. By excluding mis-alignments of optics as well as patially eliminating non-linearity of EHO mode structure as possible explanation for the data, counter-propagation observed in MIR data, which is not corroborated by external Mirnov coil array measurements, may be due to subtleties of the eigenmode structure, such as an inversion radius consistent with a magnetic island. Similar effects are observed in analysis of internal ECE-Imaging and BES data. The identification of a non-ideal structure motivates further exploration of nonlinear models of this instability. A shorter version of this contribution is due to be published in PoS at: 1st EPS conference on Plasma Diagnostics

  9. Second harmonic generation imaging of the collagen in myocardium for atrial fibrillation diagnosis

    NASA Astrophysics Data System (ADS)

    Tsai, Ming-Rung; Chiou, Yu-We; Sun, Chi-Kuang

    2009-02-01

    Myocardial fibrosis, a common sequela of cardiac hypertrophy, has been shown to be associated with arrhythmias in experimental models. Some research has indicated that myocardial fibrosis plays an important role in predisposing patients to atrial fibrillation. Second harmonic generation (SHG) is an optically nonlinear coherent process to image the collagen network. In this presentation, we observe the SHG images of the collagen matrix in atrial myocardium and we analyzed of collagen fibers arrangement by using Fourier-transform analysis. Moreover, comparing the SHG images of the collagen fibers in atrial myocardium between normal sinus rhythm (NSR) and atrial fibrillation (AF), our result indicated that it is possible to realize the relation between myocardial fibrosis and AF.

  10. Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy

    NASA Astrophysics Data System (ADS)

    Brown, R. Malcom; Millard, Andrew C.; Campagnola, Paul J.

    2003-11-01

    The macromolecular structure of purified cellulose samples is studied by second-harmonic generation (SHG) imaging microscopy. We show that the SHG contrast in both Valonia and Acetobacter cellulose strongly resembles that of collagen from animal tissues, both in terms of morphology and polarization anisotropy. Polarization analysis shows that microfibrils in each lamella are highly aligned and ordered and change directions by 90° in adjacent lamellae. The angular dependence of the SHG intensity fits well to a cos2 θ distribution, which is characteristic of the electric dipole interaction. Enzymatic degradation of Valonia fibers by cellulase is followed in real time by SHG imaging and results in exponential decay kinetics, showing that SHG imaging microscopy is ideal for monitoring dynamics in biological systems.

  11. Membrane potential dynamics of axons in cultured hippocampal neurons probed by second-harmonic-generation imaging

    NASA Astrophysics Data System (ADS)

    Nuriya, Mutsuo; Yasui, Masato

    2010-03-01

    The electrical properties of axons critically influence the nature of communication between neurons. However, due to their small size, direct measurement of membrane potential dynamics in intact and complex mammalian axons has been a challenge. Furthermore, quantitative optical measurements of axonal membrane potential dynamics have not been available. To characterize the basic principles of somatic voltage signal propagation in intact axonal arbors, second-harmonic-generation (SHG) imaging is applied to cultured mouse hippocampal neurons. When FM4-64 is applied extracellularly to dissociated neurons, whole axonal arbors are visualized by SHG imaging. Upon action potential generation by somatic current injection, nonattenuating action potentials are recorded in intact axonal arbors. Interestingly, however, both current- and voltage-clamp recordings suggest that nonregenerative subthreshold somatic voltage changes at the soma are poorly conveyed to these axonal sites. These results reveal the nature of membrane potential dynamics of cultured hippocampal neurons, and further show the possibility of SHG imaging in physiological investigations of axons.

  12. Multimodal two-photon imaging using a second harmonic generation-specific dye.

    PubMed

    Nuriya, Mutsuo; Fukushima, Shun; Momotake, Atsuya; Shinotsuka, Takanori; Yasui, Masato; Arai, Tatsuo

    2016-01-01

    Second harmonic generation (SHG) imaging can be used to visualize unique biological phenomena, but currently available dyes limit its application owing to the strong fluorescent signals that they generate together with SHG. Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no fluorescent signals, in sharp contrast to the previously used fluorescent dye FM4-64. When tested in neurons, Ap3-SHG shows linear membrane potential sensitivity and fast responses to action potentials, and also shows significantly reduced photodamage compared with FM4-64. The SHG-specific nature of Ap3 allows simultaneous and completely independent imaging of SHG signals and fluorescent signals from various reporter molecules, including markers of cellular organelles and intracellular calcium. Therefore, this SHG-specific dye enables true multimodal two-photon imaging in biological samples. PMID:27156702

  13. Multimodal two-photon imaging using a second harmonic generation-specific dye

    PubMed Central

    Nuriya, Mutsuo; Fukushima, Shun; Momotake, Atsuya; Shinotsuka, Takanori; Yasui, Masato; Arai, Tatsuo

    2016-01-01

    Second harmonic generation (SHG) imaging can be used to visualize unique biological phenomena, but currently available dyes limit its application owing to the strong fluorescent signals that they generate together with SHG. Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no fluorescent signals, in sharp contrast to the previously used fluorescent dye FM4-64. When tested in neurons, Ap3-SHG shows linear membrane potential sensitivity and fast responses to action potentials, and also shows significantly reduced photodamage compared with FM4-64. The SHG-specific nature of Ap3 allows simultaneous and completely independent imaging of SHG signals and fluorescent signals from various reporter molecules, including markers of cellular organelles and intracellular calcium. Therefore, this SHG-specific dye enables true multimodal two-photon imaging in biological samples. PMID:27156702

  14. An ab initio study of the harmonic and anharmonic force field and fundamental vibrational frequencies of performic acid

    NASA Astrophysics Data System (ADS)

    Bock, Charles W.; Trachtman, Mendel; George, Philip

    1980-11-01

    The harmonic and anharmonic force fields and fundamental vibrational frequencies of cis-cis and cis-trans performic acid are studied ab initio in the 4-31G basis set using geometries fully optimized at this level. The frequencies predicted for the cis-cis conformer are compared with those derived from spectroscopic observations on the most stable form. An extensive comparison is made between the changes in diagonal and off-diagonal quadratic and cubic force constants, and diagonal stretching quartic constants, in going from the chain to the ring structure in performic and formic acid, and features which these changes have in common are seen to support the view that there is a hydrogen bonding type of interaction in trans-formic acid despite its unfavorable geometry.

  15. Multi-oriented windowed harmonic phase reconstruction for robust cardiac strain imaging.

    PubMed

    Cordero-Grande, Lucilio; Royuela-del-Val, Javier; Sanz-Estébanez, Santiago; Martín-Fernández, Marcos; Alberola-López, Carlos

    2016-04-01

    The purpose of this paper is to develop a method for direct estimation of the cardiac strain tensor by extending the harmonic phase reconstruction on tagged magnetic resonance images to obtain more precise and robust measurements. The extension relies on the reconstruction of the local phase of the image by means of the windowed Fourier transform and the acquisition of an overdetermined set of stripe orientations in order to avoid the phase interferences from structures outside the myocardium and the instabilities arising from the application of a gradient operator. Results have shown that increasing the number of acquired orientations provides a significant improvement in the reproducibility of the strain measurements and that the acquisition of an extended set of orientations also improves the reproducibility when compared with acquiring repeated samples from a smaller set of orientations. Additionally, biases in local phase estimation when using the original harmonic phase formulation are greatly diminished by the one here proposed. The ideas here presented allow the design of new methods for motion sensitive magnetic resonance imaging, which could simultaneously improve the resolution, robustness and accuracy of motion estimates. PMID:26745763

  16. Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Mazumder, Nirmal; Qiu, Jianjun; Hu, Chih-Wei; Kao, Fu-Jen

    2013-02-01

    We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

  17. Interferometric backward third harmonic generation microscopy for axial imaging with accuracy beyond the diffraction limit.

    PubMed

    Sandkuijl, Daaf; Kontenis, Lukas; Coelho, Nuno M; McCulloch, Christopher; Barzda, Virginijus

    2014-01-01

    A new nonlinear microscopy technique based on interference of backward-reflected third harmonic generation (I-THG) from multiple interfaces is presented. The technique is used to measure height variations or changes of a layer thickness with an accuracy of up to 5 nm. Height variations of a patterned glass surface and thickness variations of fibroblasts are visualized with the interferometric epi-THG microscope with an accuracy at least two orders of magnitude better than diffraction limit. The microscopy technique can be broadly applied for measuring distance variations between membranes or multilayer structures inside biological tissue and for surface height variation imaging. PMID:24710103

  18. Cellular internalization of LiNbO3 nanocrystals for second harmonic imaging and the effects on stem cell differentiation

    NASA Astrophysics Data System (ADS)

    Li, Jianhua; Qiu, Jichuan; Guo, Weibo; Wang, Shu; Ma, Baojin; Mou, Xiaoning; Tanes, Michael; Jiang, Huaidong; Liu, Hong

    2016-03-01

    Second harmonic generation (SHG) nanocrystals have recently been reported to label cancer cells and other functional cell lines due to their unique double-frequency property. In this paper, we report for the first time the use of lithium niobate (LiNbO3, LN) nanocrystals as SHG labels for imaging stem cells. Rat mesenchymal stem cells (rMSCs) were labeled with LN nanocrystals in order to study the cellular internalization of the nanocrystals and the influence on stem cell differentiation. The results showed that LN nanocrystals were endocytosed by the rMSCs and the distribution of the internalized nanoparticles demonstrated a high consistency with the orientation of the actin filaments. Besides, LN-labeled rMSCs showed a concentration-dependent viability. Most importantly, rMSCs labeled with 50 μg per mL of LN nanocrystals retained their ability to differentiate into both osteogenic and adipogenic lineages. The results prove that LN nanocrystals can be used as a cytocompatible, near-infrared (NIR) light driven cell label for long-term imaging, without hindering stem cell differentiation. This work will promote the use of LN nanocrystals to broader applications like deep-tissue tracking, remote drug delivery and stem cell therapy.Second harmonic generation (SHG) nanocrystals have recently been reported to label cancer cells and other functional cell lines due to their unique double-frequency property. In this paper, we report for the first time the use of lithium niobate (LiNbO3, LN) nanocrystals as SHG labels for imaging stem cells. Rat mesenchymal stem cells (rMSCs) were labeled with LN nanocrystals in order to study the cellular internalization of the nanocrystals and the influence on stem cell differentiation. The results showed that LN nanocrystals were endocytosed by the rMSCs and the distribution of the internalized nanoparticles demonstrated a high consistency with the orientation of the actin filaments. Besides, LN-labeled rMSCs showed a concentration

  19. Nonlinear frequency-mixing photoacoustic imaging of a crack

    NASA Astrophysics Data System (ADS)

    Chigarev, N.; Zakrzewski, J.; Tournat, V.; Gusev, V.

    2009-08-01

    We present a technique for nonlinear photoacoustic imaging of cracks by laser excitation with intensity modulation at two fundamental frequencies combined with detection at mixed frequencies. By exploiting the strong dependence of the photoacoustic emission efficiency on the state—open or closed—of the contacts between the crack faces, remarkably enhanced image contrast is observed, ˜20 times higher than in linear photoacoustic images at the highest of the fundamental frequencies.

  20. Positional stability and radial dynamics of sonoluminescent bubbles under bi-harmonic driving: Effect of the high-frequency component and its relative phase.

    PubMed

    Rosselló, J M; Dellavale, D; Bonetto, F J

    2016-07-01

    The use of bi-frequency driving in sonoluminescence has proved to be an effective way to avoid the spatial instability (pseudo-orbits) developed by bubbles in systems with high viscous liquids like sulfuric or phosphoric acids. In this work, we present extensive experimental and numerical evidence in order to assess the effect of the high frequency component (PAc(HF)) of a bi-harmonic acoustic pressure field on the dynamic of sonoluminescent bubbles in an aqueous solution of sulfuric acid. The present study is mainly focused on the role of the harmonic frequency (Nf0) and the relative phase between the two frequency components (φb) of the acoustic field on the spatial, positional and diffusive stability of the bubbles. The results presented in this work were analyzed by means of three different approaches. First, we discussed some qualitative considerations about the changes observed in the radial dynamics, and the stability of similar bubbles under distinct bi-harmonic drivings. Later, we have investigated, through a series of numerical simulations, how the use of high frequency harmonic components of different order N, affects the positional stability of the SL bubbles. Furthermore, the influence of φb in their radius temporal evolution is systematically explored for harmonics ranging from the second to the fifteenth harmonic (N=2-15). Finally, a multivariate analysis based on the covariance method is performed to study the dependences among the parameters characterizing the SL bubble. Both experimental and numerical results indicate that the impact of PAc(HF) on the positional instability and the radial dynamics turns to be progressively negligible as the order of the high frequency harmonic component grows (i.e. N ≫ 1), however its effectiveness on the reduction of the spatial instability remains unaltered or even improved. PMID:26964988

  1. Low-Power Analog Processing for Sensing Applications: Low-Frequency Harmonic Signal Classification

    PubMed Central

    White, Daniel J.; William, Peter E.; Hoffman, Michael W.; Balkir, Sina

    2013-01-01

    A low-power analog sensor front-end is described that reduces the energy required to extract environmental sensing spectral features without using Fast Fouriér Transform (FFT) or wavelet transforms. An Analog Harmonic Transform (AHT) allows selection of only the features needed by the back-end, in contrast to the FFT, where all coefficients must be calculated simultaneously. We also show that the FFT coefficients can be easily calculated from the AHT results by a simple back-substitution. The scheme is tailored for low-power, parallel analog implementation in an integrated circuit (IC). Two different applications are tested with an ideal front-end model and compared to existing studies with the same data sets. Results from the military vehicle classification and identification of machine-bearing fault applications shows that the front-end suits a wide range of harmonic signal sources. Analog-related errors are modeled to evaluate the feasibility of and to set design parameters for an IC implementation to maintain good system-level performance. Design of a preliminary transistor-level integrator circuit in a 0.13 μm complementary metal-oxide-silicon (CMOS) integrated circuit process showed the ability to use online self-calibration to reduce fabrication errors to a sufficiently low level. Estimated power dissipation is about three orders of magnitude less than similar vehicle classification systems that use commercially available FFT spectral extraction. PMID:23892765

  2. Low-power analog processing for sensing applications: low-frequency harmonic signal classification.

    PubMed

    White, Daniel J; William, Peter E; Hoffman, Michael W; Balkir, Sina

    2013-01-01

    A low-power analog sensor front-end is described that reduces the energy required to extract environmental sensing spectral features without using Fast Fouriér Transform (FFT) or wavelet transforms. An Analog Harmonic Transform (AHT) allows selection of only the features needed by the back-end, in contrast to the FFT, where all coefficients must be calculated simultaneously. We also show that the FFT coefficients can be easily calculated from the AHT results by a simple back-substitution. The scheme is tailored for low-power, parallel analog implementation in an integrated circuit (IC). Two different applications are tested with an ideal front-end model and compared to existing studies with the same data sets. Results from the military vehicle classification and identification of machine-bearing fault applications shows that the front-end suits a wide range of harmonic signal sources. Analog-related errors are modeled to evaluate the feasibility of and to set design parameters for an IC implementation to maintain good system-level performance. Design of a preliminary transistor-level integrator circuit in a 0.13 µm complementary metal-oxide-silicon (CMOS) integrated circuit process showed the ability to use online self-calibration to reduce fabrication errors to a sufficiently low level. Estimated power dissipation is about three orders of magnitude less than similar vehicle classification systems that use commercially available FFT spectral extraction. PMID:23892765

  3. New Details of the Human Corneal Limbus Revealed With Second Harmonic Generation Imaging

    PubMed Central

    Park, Choul Yong; Lee, Jimmy K.; Zhang, Cheng; Chuck, Roy S.

    2015-01-01

    Purpose To report novel findings of the human corneal limbus by using second harmonic generation (SHG) imaging. Methods Corneal limbus was imaged by using an inverted two-photon excitation fluorescence microscope. Laser (Ti:Sapphire) was tuned at 850 nm for two-photon excitation. Backscatter signals of SHG and autofluorescence (AF) were collected through a 425/30-nm emission filter and a 525/45-emission filter, respectively. Multiple, consecutive, and overlapping image stacks (z-stack) were acquired for the corneal limbal area. Results Two novel collagen structures were revealed by SHG imaging at the limbus: an anterior limbal cribriform layer and presumed anchoring fibers. Anterior limbal cribriform layer is an intertwined reticular collagen architecture just beneath the limbal epithelial niche and is located between the peripheral cornea and Tenon's/scleral tissue. Autofluorescence imaging revealed high vascularity in this structure. Central to the anterior limbal cribriform layer, radial strands of collagen were found to connect the peripheral cornea to the limbus. These presumed anchoring fibers have both collagen and elastin and were found more extensively in the superficial layers than deep layer and were absent in very deep limbus near Schlemm's canal. Conclusions By using SHG imaging, new details of the collagen architecture of human corneal limbal area were elucidated. High resolution images with volumetric analysis revealed two novel collagen structures. PMID:26393473

  4. Gold nanocage assemblies for selective second harmonic generation imaging of cancer cell.

    PubMed

    Demeritte, Teresa; Fan, Zhen; Sinha, Sudarson Sekhar; Duan, Jinsong; Pachter, Ruth; Ray, Paresh C

    2014-01-20

    Second harmonic generation (SHG) imaging using near infrared laser light is the key to improving penetration depths, leading to biological understanding. Unfortunately, currently SHG imaging techniques have limited capability due to the poor signal-to-noise ratio, resulting from the low SHG efficiency of available dyes. Targeted tumor imaging over nontargeted tissues is also a challenge that needs to be overcome. Driven by this need, in this study, the development of two-photon SHG imaging of live cancer cell lines selectively by enhancement of the nonlinear optical response of gold nanocage assemblies is reported. Experimental results show that two-photon scattering intensity can be increased by few orders of magnitude by just developing nanoparticle self-assembly. Theoretical modeling indicates that the field enhancement values for the nanocage assemblies can explain, in part, the enhanced nonlinear optical properties. Our experimental data also show that A9 RNA aptamer conjugated gold nanocage assemblies can be used for targeted SHG imaging of the LNCaP prostate cancer cell line. Experimental results with the HaCaT normal skin cell lines show that bioconjugated nanocage-based assemblies demonstrate SHG imaging that is highly selective and will be able to distinguish targeted cancer cell lines from other nontargeted cell types. After optimization, this reported SHG imaging assay could have considerable application for biology. PMID:24339156

  5. Contrast enhancement in second harmonic imaging: discriminating between muscle and collagen

    NASA Astrophysics Data System (ADS)

    Psilodimitrakopoulos, Sotiris; Artigas, David; Soria, Guadalupe; Amat-Roldan, Ivan; Torre, Iratxe; Gratacos, Eduard; Planas, Anna M.; Loza-Alvarez, Pablo

    2009-07-01

    In this study, polarization second harmonic generation (SHG) imaging is used and data analysis is developed to gain contrast and to discriminate with pixel resolution, in the same image, SHG source architectures. We use mammalian tissue in which both skeletal muscle and fibrilar collagen can be found. The images are fitted point by point using an algorithm based on a biophysical model, where the coefficient of determination is utilized as a filtering mechanism. For the whole image we retrieve for every pixel, the effective orientation, θe , of the SHG active structures. As a result a new image is formed which its contrast depends on the values of θe . Collagen presented in the forward direction for a predefined region of interest (ROI), peak distribution of angles θe centered in the region of ~45°, while muscle in the region of ~65°. Consequently, collagen and muscle are represented in different colors in the same image. Thus, here we show that it is possible to gain contrast and to discriminate between collagen and muscle without the use of any exogenous labeling or any co-localization with fluorescence imaging.

  6. Inter- and Intra-Observer Variability in Prostate Definition With Tissue Harmonic and Brightness Mode Imaging

    SciTech Connect

    Sandhu, Gurpreet Kaur; Dunscombe, Peter; Meyer, Tyler; Pavamani, Simon; Khan, Rao

    2012-01-01

    Purpose: The objective of this study was to compare the relative utility of tissue harmonic (H) and brightness (B) transrectal ultrasound (TRUS) images of the prostate by studying interobserver and intraobserver variation in prostate delineation. Methods and Materials: Ten patients with early-stage disease were randomly selected. TRUS images of prostates were acquired using B and H modes. The prostates on all images were contoured by an experienced radiation oncologist (RO) and five equally trained observers. The observers were blinded to information regarding patient and imaging mode. The volumes of prostate glands and areas of midgland slices were calculated. Volumes contoured were compared among the observers and between observer group and RO. Contours on one patient were repeated five times by four observers to evaluate the intraobserver variability. Results: A one-sample Student t-test showed the volumes outlined by five observers are in agreement (p > 0.05) with the RO. Paired Student t-test showed prostate volumes (p = 0.008) and midgland areas (p = 0.006) with H mode were significantly smaller than that with B mode. Two-factor analysis of variances showed significant interobserver variability (p < 0.001) in prostate volumes and areas. Inter- and intraobserver consistency was quantified as the standard deviation of mean volumes and areas, and concordance indices. It was found that for small glands ({<=}35 cc) H mode provided greater interobserver consistency; however, for large glands ({>=}35 cc), B mode provided more consistent estimates. Conclusions: H mode provided superior inter- and intraobserver agreement in prostate volume definition for small to medium prostates. In large glands, H mode does not exhibit any additional advantage. Although harmonic imaging has not proven advantageous for all cases, its utilization seems to be judicious for small prostates.

  7. Biomechanical assessment and monitoring of thermal ablation using Harmonic Motion Imaging for Focused Ultrasound (HMIFU)

    NASA Astrophysics Data System (ADS)

    Hou, Gary Yi

    Cancer remains, one of the major public health problems in the United States as well as many other countries worldwide. According to According to the World Health Organization, cancer is currently the leading cause of death worldwide, accounting for 7.6 million deaths annually, and 25% of the annual death was due to Cancer during the year of 2011. In the long history of the cancer treatment field, many treatment options have been established up to date. Traditional procedures include surgical procedures as well as systemic therapies such as biologic therapy, chemotherapy, hormone therapy, and radiation therapy. Nevertheless, side-effects are often associated with such procedures due to the systemic delivery across the entire body. Recently technologies have been focused on localized therapy under minimally or noninvasive procedure with imaging-guidance, such as cryoablation, laser ablation, radio-frequency (RF) ablation, and High Intensity F-ocused Ultrasound (HIFU). HIFU is a non-invasive procedure aims to coagulate tissue thermally at a localized focal zone created with noninvasively emitting a set of focused ultrasound beams while the surrounding healthy tissues remain relatively untreated. Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a dynamic, radiation-force-based imaging technique, which utilizes a single HIFU transducer by emitting an Amplitude-modulated (AM) beam to both thermally ablate the tumor while inducing a stable oscillatory tissue displacement at its focal zone. The oscillatory response is then estimated by a cross-correlation based motion tracking technique on the signal collected by a confocally-aligned diagnostic transducer. HMIFU addresses the most critical aspect and one of the major unmet needs of HIFU treatment, which is the ability to perform real-time monitoring and mapping of tissue property change during the HIFU treatment. In this dissertation, both the assessment and monitoring aspects of HMIFU have been investigated

  8. Computational segmentation of collagen fibers from second-harmonic generation images of breast cancer.

    PubMed

    Bredfeldt, Jeremy S; Liu, Yuming; Pehlke, Carolyn A; Conklin, Matthew W; Szulczewski, Joseph M; Inman, David R; Keely, Patricia J; Nowak, Robert D; Mackie, Thomas R; Eliceiri, Kevin W

    2014-01-01

    Second-harmonic generation (SHG) imaging can help reveal interactions between collagen fibers and cancer cells. Quantitative analysis of SHG images of collagen fibers is challenged by the heterogeneity of collagen structures and low signal-to-noise ratio often found while imaging collagen in tissue. The role of collagen in breast cancer progression can be assessed post acquisition via enhanced computation. To facilitate this, we have implemented and evaluated four algorithms for extracting fiber information, such as number, length, and curvature, from a variety of SHG images of collagen in breast tissue. The image-processing algorithms included a Gaussian filter, SPIRAL-TV filter, Tubeness filter, and curvelet-denoising filter. Fibers are then extracted using an automated tracking algorithm called fiber extraction (FIRE). We evaluated the algorithm performance by comparing length, angle and position of the automatically extracted fibers with those of manually extracted fibers in twenty-five SHG images of breast cancer. We found that the curvelet-denoising filter followed by FIRE, a process we call CT-FIRE, outperforms the other algorithms under investigation. CT-FIRE was then successfully applied to track collagen fiber shape changes over time in an in vivo mouse model for breast cancer. PMID:24407500

  9. Computational segmentation of collagen fibers from second-harmonic generation images of breast cancer

    NASA Astrophysics Data System (ADS)

    Bredfeldt, Jeremy S.; Liu, Yuming; Pehlke, Carolyn A.; Conklin, Matthew W.; Szulczewski, Joseph M.; Inman, David R.; Keely, Patricia J.; Nowak, Robert D.; Mackie, Thomas R.; Eliceiri, Kevin W.

    2014-01-01

    Second-harmonic generation (SHG) imaging can help reveal interactions between collagen fibers and cancer cells. Quantitative analysis of SHG images of collagen fibers is challenged by the heterogeneity of collagen structures and low signal-to-noise ratio often found while imaging collagen in tissue. The role of collagen in breast cancer progression can be assessed post acquisition via enhanced computation. To facilitate this, we have implemented and evaluated four algorithms for extracting fiber information, such as number, length, and curvature, from a variety of SHG images of collagen in breast tissue. The image-processing algorithms included a Gaussian filter, SPIRAL-TV filter, Tubeness filter, and curvelet-denoising filter. Fibers are then extracted using an automated tracking algorithm called fiber extraction (FIRE). We evaluated the algorithm performance by comparing length, angle and position of the automatically extracted fibers with those of manually extracted fibers in twenty-five SHG images of breast cancer. We found that the curvelet-denoising filter followed by FIRE, a process we call CT-FIRE, outperforms the other algorithms under investigation. CT-FIRE was then successfully applied to track collagen fiber shape changes over time in an in vivo mouse model for breast cancer.

  10. Second Harmonic Generation Imaging Analysis of Collagen Arrangement in Human Cornea

    PubMed Central

    Park, Choul Yong; Lee, Jimmy K.; Chuck, Roy S.

    2015-01-01

    Purpose To describe the horizontal arrangement of human corneal collagen bundles by using second harmonic generation (SHG) imaging. Methods Human corneas were imaged with an inverted two photon excitation fluorescence microscope. The excitation laser (Ti:Sapphire) was tuned to 850 nm. Backscatter signals of SHG were collected through a 425/30-nm bandpass emission filter. Multiple, consecutive, and overlapping image stacks (z-stacks) were acquired to generate three dimensional data sets. ImageJ software was used to analyze the arrangement pattern (irregularity) of collagen bundles at each image plane. Results Collagen bundles in the corneal lamellae demonstrated a complex layout merging and splitting within a single lamellar plane. The patterns were significantly different in the superficial and limbal cornea when compared with deep and central regions. Collagen bundles were smaller in the superficial layer and larger in deep lamellae. Conclusions By using SHG imaging, the horizontal arrangement of corneal collagen bundles was elucidated at different depths and focal regions of the human cornea. PMID:26313297

  11. Second-harmonic and sum-frequency generation for surface studies

    SciTech Connect

    Hunt, J.H.; Guyot-Sionnest, P.; Shen, Y.R.

    1987-07-01

    Second harmonic generation (SHG) has now been well established as a versatile surface-sensitive probe. It has been used to study electrochemical processes at electrode surfaces, molecular adsorption and desorption at metal and semiconductor surfaces, orientational phase transition of molecular monolayers on water, surface reconstruction and epitaxial growth, and so on. More recently, it has been employed as a tool to monitor monolayer polymerization and other surface reactions, to probe polar order of molecules at interfaces, and to measure molecular nonlinearity. While most surface techniques are restricted to the solid/vacuum environment, SHG is applicable to nearly all interfaces as long as the interfaces are accessible by light. In addition, SHG has the advantages of being capable of in-situ measurements with high temporal, spatial, and spectral resolutions.

  12. phiFLIM: a new method to avoid aliasing in frequency-domain fluorescence lifetime imaging microscopy.

    PubMed

    Van Munster, E B; Gadella, T W J

    2004-01-01

    In conventional wide-field frequency-domain fluorescence lifetime imaging microscopy (FLIM), excitation light is intensity-modulated at megahertz frequencies. Emitted fluorescence is recorded by a CCD camera through an image intensifier, which is modulated at the same frequency. From images recorded at various phase differences between excitation and intensifier gain modulation, the phase and modulation depth of the emitted light is obtained. The fluorescence lifetime is determined from the delay and the decrease in modulation depth of the emission relative to the excitation. A minimum of three images is required, but in this case measurements become susceptible to aliasing caused by the presence of higher harmonics. Taking more images to avoid this is not always possible owing to phototoxicity or movement. A method is introduced, phiFLIM, requiring only three recordings that is not susceptible to aliasing. The phase difference between the excitation and the intensifier is scanned over the entire 360 degrees range following a predefined phase profile, during which the image produced by the intensifier is integrated onto the CCD camera, yielding a single image. Three different images are produced following this procedure, each with a different phase profile. Measurements were performed with a conventional wide-field frequency-domain FLIM system based on an acousto-optic modulator for modulation of the excitation and a microchannel-plate image intensifier coupled to a CCD camera for the detection. By analysis of the harmonic content of measured signals it was found that the third harmonic was effectively the highest present. Using the conventional method with three recordings, phase errors due to aliasing of up to +/- 29 degrees and modulation depth errors of up to 30% were found. Errors in lifetimes of YFP-transfected HeLa cells were as high as 100%. With phiFLIM, using the same specimen and settings, systematic errors due to aliasing did not occur. PMID:14678510

  13. Coherent EUV light from high-order harmonic generation: Enhancement and applications to lensless diffractive imaging

    NASA Astrophysics Data System (ADS)

    Paul, Ariel J.

    2007-12-01

    The first half of this thesis presents the first demonstration of quasi-phase matching in the coherent high-order harmonic conversion of ultrafast laser pulses into the EUV region of the spectrum. To achieve this quasi-phase matching, a novel method of fabricating hollow waveguides with a modulated inner diameter was developed. This technique lead to significant enhancements of EUV flux at wavelengths shorter than were previously accessible by known phase-matching techniques. In the second half of this thesis, the first tabletop demonstration of lensless diffractive imaging with EUV light is presented using HHG in a gas-filled hollow waveguide to provide coherent illumination. This tabletop microscope shows a spatial resolution of ˜ 200 nm and a large depth of field. Furthermore, the technique is easily scalable to shorter wavelengths of interest to biological imaging.

  14. Polarization sensitive optical frequency domain imaging system for endobronchial imaging.

    PubMed

    Li, Jianan; Feroldi, Fabio; de Lange, Joop; Daniels, Johannes M A; Grünberg, Katrien; de Boer, Johannes F

    2015-02-01

    A polarization sensitive endoscopic optical frequency domain imaging (PS-OFDI) system with a motorized distal scanning catheter is demonstrated. It employs a passive polarization delay unit to multiplex two orthogonal probing polarization states in depth, and a polarization diverse detection unit to detect interference signal in two orthogonal polarization channels. Per depth location four electro-magnetic field components are measured that can be represented in a complex 2x2 field matrix. A Jones matrix of the sample is derived and the sample birefringence is extracted by eigenvalue decomposition. The condition of balanced detection and the polarization mode dispersion are quantified. A complex field averaging method based on the alignment of randomly pointing field phasors is developed to reduce speckle noise. The variation of the polarization states incident on the tissue due to the circular scanning and catheter sheath birefringence is investigated. With this system we demonstrated imaging of ex vivo chicken muscle, in vivo pig lung and ex vivo human lung specimens. PMID:25836196

  15. Imaging scattering orientation with spatial frequency domain imaging

    PubMed Central

    Konecky, Soren D.; Rice, Tyler; Durkin, Anthony J.; Tromberg, Bruce J.

    2011-01-01

    Optical imaging techniques based on multiple light scattering generally have poor sensitivity to the orientation and direction of microscopic light scattering structures. In order to address this limitation, we introduce a spatial frequency domain method for imaging contrast from oriented scattering structures by measuring the angular-dependence of structured light reflectance. The measurement is made by projecting sinusoidal patterns of light intensity on a sample, and measuring the degree to which the patterns are blurred as a function of the projection angle. We derive a spatial Fourier domain solution to an anisotropic diffusion model. This solution predicts the effects of bulk scattering orientation on the amplitude and phase of the projected patterns. We introduce a new contrast function based on a scattering orientation index (SOI) which is sensitive to the degree to which light scattering is directionally dependent. We validate the technique using tissue simulating phantoms, and ex vivo samples of muscle and brain. Our results show that SOI is independent of the overall amount of bulk light scattering and absorption, and that isotropic versus oriented scattering structures can be clearly distinguished. We determine the orientation of subsurface microscopic scattering structures located up to 600 μm beneath highly scattering (μ′s = 1.5 mm−1) material. PMID:22191918

  16. Design of a Second Harmonic Double-Beam Continuous Wave Gyrotron with Operating Frequency of 0.79 THz

    NASA Astrophysics Data System (ADS)

    Manuilov, V. N.; Glyavin, M. Yu; Sedov, A. S.; Zaslavsky, V. Yu; Idehara, T.

    2015-12-01

    This paper presents the most essential steps of a design study of a novel second harmonic gyrotron operating in CW (continuous wave) regime at a frequency of 0.79 THz and an output power of 1-100 W. It is based on a novel idea for suppression of the parasitic modes using a double-beam electron-optical system (EOS). It includes a triode magnetron injection gun (MIG), which forms two high-quality helical electron beams (HEB). Different schemes, namely one with two generating beams and another with one generating and one absorbing beam, have been investigated and compared. It has been shown that the scheme with two generating beams is more advantageous since it allows an effective suppression of the parasitic modes and a stable single-mode operation at the second harmonic resonance. A MIG which is appropriate for the realization of the latter scheme has been optimized using numerical codes for computer-aided design (CAD). It forms beams with practically equal pitch factors and moderate velocity spread. The construction of the gun is not sensitive to small misalignments and shifts of the electrodes and the magnetic field. Among the most promising characteristics of the presented design are an improved mode selection and a stable single-mode generation at currents that are two to three times higher than the currents in the single-beam (i.e., conventional) gyrotrons.

  17. Cellular internalization of LiNbO3 nanocrystals for second harmonic imaging and the effects on stem cell differentiation.

    PubMed

    Li, Jianhua; Qiu, Jichuan; Guo, Weibo; Wang, Shu; Ma, Baojin; Mou, Xiaoning; Tanes, Michael; Jiang, Huaidong; Liu, Hong

    2016-03-31

    Second harmonic generation (SHG) nanocrystals have recently been reported to label cancer cells and other functional cell lines due to their unique double-frequency property. In this paper, we report for the first time the use of lithium niobate (LiNbO3, LN) nanocrystals as SHG labels for imaging stem cells. Rat mesenchymal stem cells (rMSCs) were labeled with LN nanocrystals in order to study the cellular internalization of the nanocrystals and the influence on stem cell differentiation. The results showed that LN nanocrystals were endocytosed by the rMSCs and the distribution of the internalized nanoparticles demonstrated a high consistency with the orientation of the actin filaments. Besides, LN-labeled rMSCs showed a concentration-dependent viability. Most importantly, rMSCs labeled with 50 μg per mL of LN nanocrystals retained their ability to differentiate into both osteogenic and adipogenic lineages. The results prove that LN nanocrystals can be used as a cytocompatible, near-infrared (NIR) light driven cell label for long-term imaging, without hindering stem cell differentiation. This work will promote the use of LN nanocrystals to broader applications like deep-tissue tracking, remote drug delivery and stem cell therapy. PMID:27001708

  18. Applications of Second-Harmonic Generation Imaging Microscopy in Ovarian and Breast Cancer

    PubMed Central

    Tilbury, Karissa; Campagnola, Paul J

    2015-01-01

    In this perspective, we discuss how the nonlinear optical technique of second-harmonic generation (SHG) microscopy has been used to greatly enhance our understanding of the tumor microenvironment (TME) of breast and ovarian cancer. Striking changes in collagen architecture are associated with these epithelial cancers, and SHG can image these changes with great sensitivity and specificity with submicrometer resolution. This information has not historically been exploited by pathologists but has the potential to enhance diagnostic and prognostic capabilities. We summarize the utility of image processing tools that analyze fiber morphology in SHG images of breast and ovarian cancer in human tissues and animal models. We also describe methods that exploit the SHG physical underpinnings that are effective in delineating normal and malignant tissues. First we describe the use of polarization-resolved SHG that yields metrics related to macromolecular and supramolecular structures. The coherence and corresponding phase-matching process of SHG results in emission directionality (forward to backward), which is related to sub-resolution fibrillar assembly. These analyses are more general and more broadly applicable than purely morphology-based analyses; however, they are more computationally intensive. Intravital imaging techniques are also emerging that incorporate all of these quantitative analyses. Now, all these techniques can be coupled with rapidly advancing miniaturization of imaging systems to afford their use in clinical situations including enhancing pathology analysis and also in assisting in real-time surgical determination of tumor margins. PMID:25987830

  19. Molecular probes for two-photon excited fluorescence and second harmonic generation imaging of biological membranes

    NASA Astrophysics Data System (ADS)

    Porres, Laurent; Mongin, Olivier; Bhatthula, Bharath K. G.; Blanchard-Desce, Mireille H.; Ventelon, Lionel; Moreaux, Laurent; Pons, T.; Mertz, Jerome

    2002-11-01

    Novel microscopies based on nonlinear optical (NLO) phenomena are attracting increasing interest in the biology community owing to their potentialities in the area of real-time, non-damaging imaging of biological systems. In particular, second-harmonic generation (SHG) and two-photon excited fluorescence (TPEF) are NLO phenomena that scale with excitation intensity squared, and thus give rise to an intrinsic 3-dimensional resolution when used in microscopic imaging. In this perspective, we have implemented a molecular engineering approach toward NLO-probes specifically designed for SHG and/or TPEF imaging of cellular membranes. We have designed nanoscale rod-like fluorophores showing very large TPEF cross-sections in the visible red, outperforming standard fluorophores such as fluorescein by up to two orders of magnitude. Bolaamphiphilic derivatives combining high TPEF cross-sections and affinity for cellular membranes were prepared. Their incorporation into model or cell membranes can be monitored by TPEF microscopy. Amphiphilic push-pull chromophores showing both high TPA and SHG cross-sections in the near-IR region were designed as NLO-probes for imaging of biological membranes by simultaneous SHG and TPEF microscopy. These NLO-phores offer intriguing potentialities for imaging of fundamental biological processes such as adhesion, fusion or for reporting of membrane electrical potentials.

  20. Clinical evaluation of synthetic aperture harmonic imaging for scanning focal malignant liver lesions.

    PubMed

    Brandt, Andreas Hjelm; Hemmsen, Martin Christian; Hansen, Peter Møller; Madsen, Signe Sloth; Krohn, Paul Suno; Lange, Theis; Hansen, Kristoffer Lindskov; Jensen, Jørgen Arendt; Nielsen, Michael Bachmann

    2015-09-01

    The purpose of the study was to perform a clinical comparison of synthetic aperture sequential beamforming tissue harmonic imaging (SASB-THI) sequences with a conventional imaging technique, dynamic receive focusing with THI (DRF-THI). Both techniques used pulse inversion and were recorded interleaved using a commercial ultrasound system (UltraView 800, BK Medical, Herlev, Denmark). Thirty-one patients with malignant focal liver lesions (confirmed by biopsy or computed tomography/magnetic resonance) were scanned. Detection of malignant focal liver lesions and preference of image quality were evaluated blinded off-line by eight radiologists. In total, 2,032 evaluations of 127 image sequences were completed. The sensitivity (77% SASB-THI, 76% DRF-THI, p = 0.54) and specificity (71% SASB-THI, 72% DRF-THI, p = 0.67) of detection of liver lesions and the evaluation of image quality (p = 0.63) did not differ between SASB-THI and DRF-THI. This study indicates the ability of SASB-THI in a true clinical setting. PMID:26095533

  1. Applications of second-harmonic generation imaging microscopy in ovarian and breast cancer.

    PubMed

    Tilbury, Karissa; Campagnola, Paul J

    2015-01-01

    In this perspective, we discuss how the nonlinear optical technique of second-harmonic generation (SHG) microscopy has been used to greatly enhance our understanding of the tumor microenvironment (TME) of breast and ovarian cancer. Striking changes in collagen architecture are associated with these epithelial cancers, and SHG can image these changes with great sensitivity and specificity with submicrometer resolution. This information has not historically been exploited by pathologists but has the potential to enhance diagnostic and prognostic capabilities. We summarize the utility of image processing tools that analyze fiber morphology in SHG images of breast and ovarian cancer in human tissues and animal models. We also describe methods that exploit the SHG physical underpinnings that are effective in delineating normal and malignant tissues. First we describe the use of polarization-resolved SHG that yields metrics related to macromolecular and supramolecular structures. The coherence and corresponding phase-matching process of SHG results in emission directionality (forward to backward), which is related to sub-resolution fibrillar assembly. These analyses are more general and more broadly applicable than purely morphology-based analyses; however, they are more computationally intensive. Intravital imaging techniques are also emerging that incorporate all of these quantitative analyses. Now, all these techniques can be coupled with rapidly advancing miniaturization of imaging systems to afford their use in clinical situations including enhancing pathology analysis and also in assisting in real-time surgical determination of tumor margins. PMID:25987830

  2. Coherent control of multiphoton dynamics and high-order-harmonic generation driven by two frequency-comb fields with a relative envelope delay

    NASA Astrophysics Data System (ADS)

    Zhao, Di; Jiang, Chen-Wei; Li, Fu-li

    2016-07-01

    We present a theoretical investigation of the coherent control of multiphoton dynamics and a high-order-harmonic generation (HHG) process driven by two frequency-comb fields, via the interference of multiphoton transition paths by tuning the relative envelope delay between fields. The many-mode Floquet theorem is employed to provide a nonperturbative and exact treatment of the interaction between a quantum system and frequency-comb laser fields. The case of two frequency-comb fields with the same repetition frequency and the carrier frequencies of fundamental and second harmonics, respectively, is considered. Due to the coupling of the second harmonic controlling the frequency-comb laser field, multiphoton transitions involving both fundamental- and second-harmonic photons occur. Different multiphoton transition paths can be superpositioned when the matching condition for carrier-envelope-phase shifts is satisfied, offering the possibility of coherent control of HHG power spectra via the interference of paths by tuning the relative envelope delay between fields. The calculated HHG power spectra present both sub-cycle oscillation and multi-cycle modulation behavior when the relative envelope delay is varied. It is also found that, under the condition of multiphoton resonance, the HHG power spectra can be further enhanced by about 10 times via the interference of multiphoton transition paths by tuning the relative envelope delay.

  3. Elasticity mapping of murine abdominal organs in vivo using harmonic motion imaging (HMI)

    NASA Astrophysics Data System (ADS)

    Payen, Thomas; Palermo, Carmine F.; Sastra, Stephen A.; Chen, Hong; Han, Yang; Olive, Kenneth P.; Konofagou, Elisa E.

    2016-08-01

    Recently, ultrasonic imaging of soft tissue mechanics has been increasingly studied to image otherwise undetectable pathologies. However, many underlying mechanisms of tissue stiffening remain unknown, requiring small animal studies and adapted elasticity mapping techniques. Harmonic motion imaging (HMI) assesses tissue viscoelasticity by inducing localized oscillation from a periodic acoustic radiation force. The objective of this study was to evaluate the feasibility of HMI for in vivo elasticity mapping of abdominal organs in small animals. Pathological cases, i.e. chronic pancreatitis and pancreatic cancer, were also studied in vivo to assess the capability of HMI for detection of the change in mechanical properties. A 4.5 MHz focused ultrasound transducer (FUS) generated an amplitude-modulated beam resulting in 50 Hz harmonic tissue oscillations at its focus. Axial tissue displacement was estimated using 1D-cross-correlation of RF signals acquired with a 7.8 MHz diagnostic transducer confocally aligned with the FUS. In vitro results in canine liver and kidney showed the correlation between HMI displacement and Young’s moduli measured by rheometry compression testing. HMI was capable of providing reproducible elasticity maps of the mouse abdominal region in vivo allowing the identification of, from stiffest to softest, the murine kidney, pancreas, liver, and spleen. Finally, pancreata affected by pancreatitis and pancreatic cancer showed HMI displacements 1.7 and 2.2 times lower than in the control case, respectively, indicating higher stiffness. The HMI displacement amplitude was correlated with the extent of fibrosis as well as detecting the very onset of stiffening even before fibrosis could be detected on H&E. This work shows that HMI can produce reliable elasticity maps of mouse abdominal region in vivo, thus providing a potentially critical tool to assess pathologies affecting organ elasticity.

  4. Elasticity mapping of murine abdominal organs in vivo using harmonic motion imaging (HMI).

    PubMed

    Payen, Thomas; Palermo, Carmine F; Sastra, Stephen A; Chen, Hong; Han, Yang; Olive, Kenneth P; Konofagou, Elisa E

    2016-08-01

    Recently, ultrasonic imaging of soft tissue mechanics has been increasingly studied to image otherwise undetectable pathologies. However, many underlying mechanisms of tissue stiffening remain unknown, requiring small animal studies and adapted elasticity mapping techniques. Harmonic motion imaging (HMI) assesses tissue viscoelasticity by inducing localized oscillation from a periodic acoustic radiation force. The objective of this study was to evaluate the feasibility of HMI for in vivo elasticity mapping of abdominal organs in small animals. Pathological cases, i.e. chronic pancreatitis and pancreatic cancer, were also studied in vivo to assess the capability of HMI for detection of the change in mechanical properties. A 4.5 MHz focused ultrasound transducer (FUS) generated an amplitude-modulated beam resulting in 50 Hz harmonic tissue oscillations at its focus. Axial tissue displacement was estimated using 1D-cross-correlation of RF signals acquired with a 7.8 MHz diagnostic transducer confocally aligned with the FUS. In vitro results in canine liver and kidney showed the correlation between HMI displacement and Young's moduli measured by rheometry compression testing. HMI was capable of providing reproducible elasticity maps of the mouse abdominal region in vivo allowing the identification of, from stiffest to softest, the murine kidney, pancreas, liver, and spleen. Finally, pancreata affected by pancreatitis and pancreatic cancer showed HMI displacements 1.7 and 2.2 times lower than in the control case, respectively, indicating higher stiffness. The HMI displacement amplitude was correlated with the extent of fibrosis as well as detecting the very onset of stiffening even before fibrosis could be detected on H&E. This work shows that HMI can produce reliable elasticity maps of mouse abdominal region in vivo, thus providing a potentially critical tool to assess pathologies affecting organ elasticity. PMID:27401609

  5. Digital pathology and image analysis augment biospecimen annotation and biobank quality assurance harmonization

    PubMed Central

    Wei, Bih-Rong; Simpson, R. Mark

    2015-01-01

    Standardization of biorepository best practices will enhance the quality of translational biomedical research utilizing patient-derived biobank specimens. Harmonization of pathology quality assurance procedures for biobank accessions has lagged behind other avenues of biospecimen research and biobank development. Comprehension of the cellular content of biorepository specimens is important for discovery of tissue-specific clinically relevant biomarkers for diagnosis and treatment. While rapidly emerging technologies in molecular analyses and data mining create focus on appropriate measures for minimizing pre-analytic artifact-inducing variables, less attention gets paid to annotating the constituent make up of biospecimens for more effective specimen selection by biobank clients. Both pre-analytic tissue processing and a specimen's composition influence acquisition of relevant macromolecules for downstream assays. Pathologist review of biorepository submissions, particularly tissues as part of quality assurance procedures, helps to ensure that the intended target cells are present and in sufficient quantity in accessioned specimens. This manual procedure can be tedious and subjective. Incorporating digital pathology into biobank quality assurance procedures, using automated pattern recognition morphometric image analysis to quantify tissue feature areas in digital whole slide images of tissue sections, can minimize variability and subjectivity associated with routine pathologic evaluations in biorepositories. Whole-slide images and pathologist-reviewed morphometric analyses can be provided to researchers to guide specimen selection. Harmonization of pathology quality assurance methods that minimize subjectivity and improve reproducibility among collections would facilitate research-relevant specimen selection by investigators and could facilitate information sharing in an integrated network approach to biobanking. PMID:24362266

  6. Synergy in Two-Frequency Fast Wave Cyclotron Harmonic Absorption in DIII-D

    SciTech Connect

    Pinsker, R. I.; Choi, M.; Heidbrink, W. W.; Zhu, Y.; Porkolab, M.; Baity, F. W.; Hosea, J. C.

    2009-11-26

    Fast waves (FWs) at 60 MHz and at 90 MHz are coupled to DIII-D discharges for central heating and current drive at net FW power up to 3.5 MW. The primary absorption mechanism is intended to be direct electron damping in the plasma core. In discharges at B = 2 T with fast deuteron populations from neutral beam injection, 4th and 6th deuterium cyclotron harmonic absorption on the fast ions competes with direct electron damping. Previous experiments have shown that the 6{omega}{sub D} absorption of the 90 MHz FWs is weaker than the 4{omega}{sub D} absorption of 60 MHz FWs, in agreement with a model that includes unspecified edge losses. Recent experiments have shown that if the fast deuterons are accelerated by absorption of 60 MHz (4{omega}{sub D}) FWs, adding 90 MHz power (6{omega}{sub D}) can increase the fusion neutron rate by a larger increment than is obtained with 90 MHz power alone. Details of this synergy between 4{omega}{sub D} and 6{omega}{sub D} absorption are presented.

  7. Removing the depth-degeneracy in optical frequency domain imaging with frequency shifting

    PubMed Central

    Yun, S. H.; Tearney, G. J.; de Boer, J. F.; Bouma, B. E.

    2009-01-01

    A novel technique using an acousto-optic frequency shifter in optical frequency domain imaging (OFDI) is presented. The frequency shift eliminates the ambiguity between positive and negative differential delays, effectively doubling the interferometric ranging depth while avoiding image cross-talk. A signal processing algorithm is demonstrated to accommodate nonlinearity in the tuning slope of the wavelength-swept OFDI laser source. PMID:19484034

  8. Spatial frequency bandwidth used in the recognition of facial images.

    PubMed

    Näsänen, R

    1999-11-01

    The purpose of the study was to find out what spatial frequency information human observers use in the recognition of face images. Signal-to-noise ratio thresholds for the recognition of facial images were measured as a function of the centre spatial frequency of narrow-band additive spatial noise. The relative sensitivity of recognition to different spatial frequencies was derived from these results. The maximum sensitivity was found at 8-13 c/face width and the bandwidth was just under two octaves. Qualitatively similar results were obtained with stimuli in which Fourier phase was randomised within a narrow band of different centre spatial frequencies. This resulted in a considerable increase of energy threshold around 8 c/face width and less elsewhere. Further, contrast energy thresholds were measured as a function of the centre spatial frequency of band-pass filtered face images. As a function of object spatial frequency (c/face width), energy threshold first decreased and then increased. The lowest energy thresholds found around 10 c/face width were lower than the energy threshold for unfiltered images. This is what one would expect if face recognition is narrow-band, since band-pass filtered images of optimal centre spatial frequency do not contain unused contrast energy at low and high spatial frequencies. In conclusion, the results suggest that the recognition of facial images is tuned to a relatively narrow band (< 2 octaves) of mid object spatial frequencies. PMID:10748918

  9. Thin and Slow Smoke Detection by Using Frequency Image

    NASA Astrophysics Data System (ADS)

    Zheng, Guang; Oe, Shunitiro

    In this paper, a new method to detect thin and slow smoke for early fire alarm by using frequency image has been proposed. The correlation coefficient of the frequency image between the current stage and the initial stage are calculated, so are the gray image correlation coefficient of the color image. When the thin smoke close to transparent enters into the camera view, the correlation coefficient of the frequency image becomes small, while the gray image correlation coefficient of the color image hardly change and keep large. When something which is not transparent, like human beings, etc., enters into the camera view, the correlation coefficient of the frequency image becomes small, as well as that of color image. Based on the difference of correlation coefficient between frequency image and color image in different situations, the thin smoke can be detected. Also, considering the movement of the thin smoke, miss detection caused by the illustration change or noise can be avoided. Several experiments in different situations are carried out, and the experimental results show the effect of the proposed method.

  10. Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation

    NASA Astrophysics Data System (ADS)

    Vuillemin, Nelly; Mahou, Pierre; Débarre, Delphine; Gacoin, Thierry; Tharaux, Pierre-Louis; Schanne-Klein, Marie-Claire; Supatto, Willy; Beaurepaire, Emmanuel

    2016-07-01

    Second-harmonic generation (SHG) is the most specific label-free indicator of collagen accumulation in widespread pathologies such as fibrosis, and SHG-based measurements hold important potential for biomedical analyses. However, efficient collagen SHG scoring in histological slides is hampered by the limited depth-of-field of usual nonlinear microscopes relying on focused Gaussian beam excitation. In this work we analyze theoretically and experimentally the use of Bessel beam excitation to address this issue. Focused Bessel beams can provide an axially extended excitation volume for nonlinear microscopy while preserving lateral resolution. We show that shaping the focal volume has consequences on signal level and scattering directionality in the case of coherent signals (such as SHG) which significantly differ from the case of incoherent signals (two-photon excited fluorescence, 2PEF). We demonstrate extended-depth SHG-2PEF imaging of fibrotic mouse kidney histological slides. Finally, we show that Bessel beam excitation combined with spatial filtering of the harmonic light in wave vector space can be used to probe collagen accumulation more efficiently than the usual Gaussian excitation scheme. These results open the way to SHG-based histological diagnoses.

  11. Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation

    PubMed Central

    Vuillemin, Nelly; Mahou, Pierre; Débarre, Delphine; Gacoin, Thierry; Tharaux, Pierre-Louis; Schanne-Klein, Marie-Claire; Supatto, Willy; Beaurepaire, Emmanuel

    2016-01-01

    Second-harmonic generation (SHG) is the most specific label-free indicator of collagen accumulation in widespread pathologies such as fibrosis, and SHG-based measurements hold important potential for biomedical analyses. However, efficient collagen SHG scoring in histological slides is hampered by the limited depth-of-field of usual nonlinear microscopes relying on focused Gaussian beam excitation. In this work we analyze theoretically and experimentally the use of Bessel beam excitation to address this issue. Focused Bessel beams can provide an axially extended excitation volume for nonlinear microscopy while preserving lateral resolution. We show that shaping the focal volume has consequences on signal level and scattering directionality in the case of coherent signals (such as SHG) which significantly differ from the case of incoherent signals (two-photon excited fluorescence, 2PEF). We demonstrate extended-depth SHG-2PEF imaging of fibrotic mouse kidney histological slides. Finally, we show that Bessel beam excitation combined with spatial filtering of the harmonic light in wave vector space can be used to probe collagen accumulation more efficiently than the usual Gaussian excitation scheme. These results open the way to SHG-based histological diagnoses. PMID:27435390

  12. Selective imaging in second-harmonic-generation microscopy with anisotropic radiation.

    PubMed

    Chu, Shi-Wei; Tai, Shih-Peng; Liu, Tzu-Ming; Sun, Chi-Kuang; Lin, Chi-Hung

    2009-01-01

    As a novel modality of optical microscopy, second-harmonic generation (SHG) provides attractive features including intrinsic optical sectioning, noninvasiveness, high specificity, and high penetrability. For a biomedical application, the epicollection of backward propagating SHG is necessary. But due to phase-matching constraint, SHG from thick tissues is preferentially forward propagation. Myosin and collagen are two of the most abundant fibrous proteins in vertebrates, and both exhibit a strong second-harmonic response. We find that the radiation patterns of myosin-based muscle fibers and collagen fibrils are distinct due to coherence effects. Based on these asymmetric radiation patterns, we demonstrate selective imaging between intertwining muscle fibers and type I collagen fibrils with forward and backward SHG modalities, respectively. Thick muscle fibers dominate the forward signal, while collagen fibril distribution is preferentially resolved in the backward channel without strong interference from muscle. Moreover, we find that well-formed collagen fibrils are highlighted by forward SHG, while loosely arranged collagen matrix is outlined by backward signal. PMID:19256686

  13. Theoretical analysis of dynamic chemical imaging with lasers using high-order harmonic generation

    SciTech Connect

    Van-Hoang Le; Anh-Thu Le; Xie Ruihua; Lin, C. D.

    2007-07-15

    We report theoretical investigations of the tomographic procedure suggested by Itatani et al. [Nature (London) 432, 867 (2004)] for reconstructing highest occupied molecular orbitals (HOMOs) using high-order harmonic generation (HHG). Due to the limited range of harmonics from the plateau region, we found that even under the most favorable assumptions, it is still very difficult to obtain accurate HOMO wave functions using the tomographic procedure, but the symmetry of the HOMOs and the internuclear separation between the atoms can be accurately extracted, especially when lasers of longer wavelengths are used to generate the HHG. Since the tomographic procedure relies on approximating the continuum wave functions in the recombination process by plane waves, the method can no longer be applied upon the improvement of the theory. For future chemical imaging with lasers, we suggest that one may want to focus on how to extract the positions of atoms in molecules instead, by developing an iterative method such that the theoretically calculated macroscopic HHG spectra can best fit the experimental HHG data.

  14. Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation.

    PubMed

    Vuillemin, Nelly; Mahou, Pierre; Débarre, Delphine; Gacoin, Thierry; Tharaux, Pierre-Louis; Schanne-Klein, Marie-Claire; Supatto, Willy; Beaurepaire, Emmanuel

    2016-01-01

    Second-harmonic generation (SHG) is the most specific label-free indicator of collagen accumulation in widespread pathologies such as fibrosis, and SHG-based measurements hold important potential for biomedical analyses. However, efficient collagen SHG scoring in histological slides is hampered by the limited depth-of-field of usual nonlinear microscopes relying on focused Gaussian beam excitation. In this work we analyze theoretically and experimentally the use of Bessel beam excitation to address this issue. Focused Bessel beams can provide an axially extended excitation volume for nonlinear microscopy while preserving lateral resolution. We show that shaping the focal volume has consequences on signal level and scattering directionality in the case of coherent signals (such as SHG) which significantly differ from the case of incoherent signals (two-photon excited fluorescence, 2PEF). We demonstrate extended-depth SHG-2PEF imaging of fibrotic mouse kidney histological slides. Finally, we show that Bessel beam excitation combined with spatial filtering of the harmonic light in wave vector space can be used to probe collagen accumulation more efficiently than the usual Gaussian excitation scheme. These results open the way to SHG-based histological diagnoses. PMID:27435390

  15. Improved Estimation of Ultrasound Thermal Strain Using Pulse Inversion Harmonic Imaging.

    PubMed

    Ding, Xuan; Nguyen, Man M; James, Isaac B; Marra, Kacey G; Rubin, J Peter; Leers, Steven A; Kim, Kang

    2016-05-01

    Thermal (temporal) strain imaging (TSI) is being developed to detect the lipid-rich core of atherosclerotic plaques and presence of fatty liver disease. However, the effects of ultrasonic clutter on TSI have not been considered. In this study, we evaluated whether pulse inversion harmonic imaging (PIHI) could be used to improve estimates of thermal (temporal) strain. Using mixed castor oil-gelatin phantoms of different concentrations and artificially introduced clutter, we found that PIHI improved the signal-to-noise ratio of TSI by an average of 213% or 52.1% relative to 3.3- and 6.6-MHz imaging, respectively. In a phantom constructed using human liposuction fat in the presence of clutter, the contrast-to-noise ratio was degraded by 35.1% for PIHI compared with 62.4% and 43.7% for 3.3- and 6.6-MHz imaging, respectively. These findings were further validated using an ex vivo carotid endarterectomy sample. PIHI can be used to improve estimates of thermal (temporal) strain in the presence of clutter. PMID:26948260

  16. Internal frequency conversion extreme ultraviolet interferometer using mutual coherence properties of two high-order-harmonic sources.

    PubMed

    Dobosz, S; Stabile, H; Tortora, A; Monot, P; Réau, F; Bougeard, M; Merdji, H; Carré, B; Martin, Ph; Joyeux, D; Phalippou, D; Delmotte, F; Gautier, J; Mercier, R

    2009-11-01

    We report on an innovative two-dimensional imaging extreme ultraviolet (XUV) interferometer operating at 32 nm based on the mutual coherence of two laser high order harmonics (HOH) sources, separately generated in gas. We give the first evidence that the two mutually coherent HOH sources can be produced in two independent spatially separated gas jets, allowing for probing centimeter-sized objects. A magnification factor of 10 leads to a micron resolution associated with a subpicosecond temporal resolution. Single shot interferograms with a fringe visibility better than 30% are routinely produced. As a test of the XUV interferometer, we measure a maximum electronic density of 3x10(20) cm(-3) 1.1 ns after the creation of a plasma on aluminum target. PMID:19947712

  17. Internal frequency conversion extreme ultraviolet interferometer using mutual coherence properties of two high-order-harmonic sources

    SciTech Connect

    Dobosz, S.; Stabile, H.; Tortora, A.; Monot, P.; Reau, F.; Bougeard, M.; Merdji, H.; Carre, B.; Martin, Ph.; Joyeux, D.; Phalippou, D.; Delmotte, F.; Gautier, J.; Mercier, R.

    2009-11-15

    We report on an innovative two-dimensional imaging extreme ultraviolet (XUV) interferometer operating at 32 nm based on the mutual coherence of two laser high order harmonics (HOH) sources, separately generated in gas. We give the first evidence that the two mutually coherent HOH sources can be produced in two independent spatially separated gas jets, allowing for probing centimeter-sized objects. A magnification factor of 10 leads to a micron resolution associated with a subpicosecond temporal resolution. Single shot interferograms with a fringe visibility better than 30% are routinely produced. As a test of the XUV interferometer, we measure a maximum electronic density of 3x10{sup 20} cm{sup -3} 1.1 ns after the creation of a plasma on aluminum target.

  18. Monitoring process of human keloid formation based on second harmonic generation imaging

    NASA Astrophysics Data System (ADS)

    Jiang, X. S.; Chen, S.; Chen, J. X.; Zhu, X. Q.; Zheng, L. Q.; Zhuo, S. M.; Wang, D. J.

    2011-09-01

    In this paper, the morphological variation of collagen among the whole dermis from keloid tissue was investigated using second harmonic generation (SHG) microscopy. In the deep dermis of keloids, collagen bundles show apparently regular gap. In the middle dermis, the collagen bundles are randomly oriented and loosely arranged in the pattern of fine mesh while the collagen bundles are organized in a parallel manner in the superficial dermis near the epidermis. The developed parameters COI and BD can be used to further quantitatively describe these changes. Our results demonstrate the potential of SHG microscopy to understand the formation process of human keloid scar at the cellular level through imaging collagen variations in different depth of dermis.

  19. Polarization response of second-harmonic images for different collagen spatial distributions

    NASA Astrophysics Data System (ADS)

    Ávila, Francisco J.; del Barco, Oscar; Bueno, Juan M.

    2016-06-01

    The response to polarization of second-harmonic generation (SHG) microscopy images of samples with different collagen distributions (quasialigned, partially organized, and nonorganized) has been analyzed. A linear decay relationship between the external arrangement and polarization sensitivity was found. SHG signal from nonorganized samples presented a large structural dispersion and a weak dependence with incident polarization. Polarization dependence is also associated with the internal organization of the collagen fibers, directly related to the ratio of hyperpolarizabilities ρ. This parameter can experimentally be computed from the modulation of the SHG signal. The results show that both external and internal collagen structures are closely related. This provides a tool to obtain information of internal properties from the polarimetric response of the external spatial distribution of collagen, which might be useful in clinical diagnosis of pathologies related to changes in collagen structure.

  20. Molecular Order of Arterial Collagen Using Circular Polarization Second-Harmonic Generation Imaging.

    PubMed

    Turcotte, Raphaël; Mattson, Jeffrey M; Wu, Juwell W; Zhang, Yanhang; Lin, Charles P

    2016-02-01

    Second-harmonic generation (SHG) originates from the interaction between upconverted fields from individual scatterers. This renders SHG microscopy highly sensitive to molecular distribution. Here, we aim to take advantage of the difference in SHG between aligned and partially aligned molecules to probe the degree of molecular order during biomechanical testing, independently of the absolute orientation of the scattering molecules. Toward this goal, we implemented a circular polarization SHG imaging approach and used it to quantify the intensity change associated with collagen fibers straightening in the arterial wall during mechanical stretching. We were able to observe the delayed alignment of collagen fibers during mechanical loading, thus demonstrating a simple method to characterize molecular distribution using intensity information alone. PMID:26806883

  1. First and second harmonic ECRH experience at gyrotron frequencies at LLNL

    SciTech Connect

    Stallard, B.

    1987-11-01

    Plasma heating of electrons in both mirror machines and tokamaks, using mm wave gyrotron sources, have been carried out in many experiments in recent years. The technology for both sources and mode-preserving waveguide transmission systems is well developed at power levels of 200 kW. At LLNL electron heating at 28 GHz in the TMX-U tandem mirror has been used to create hot electrons required for a thermal barrier (potential well). TMX-U, and other devices operating at lower frequency and power (10 GHz, few kW), routinely generates electron populations with mean energies of 100 to 500 keV and densities in the low to mid 10/sup 11/ cm/sup -3/ range. Radial pressure profiles vary from peaked-on-axis to hollow and are dependent on the mod-B resonance surfaces. Experiments on the axisymmetric mirror SM-1 have shown improved heating efficiency using multiple frequencies with narrow frequency separation. The importance of rf diffusion in determining electron confinement has been shown in CONSTANCE B. Fokker-Planck and particle orbit models have been useful for understanding the importance of cavity heating for creating runaway electrons, the sensitivity of hot electron production to cold plasma, the reduction of electron lifetime by rf diffusion, and the effect of multiple frequencies on heating stochasticity. Potential wells generated in plasmas with large fractions of mirror-trapped electrons have been measured in TMX-U. These offer prospects for enhanced confinement of highly stripped ions. 11 refs., 18 figs., 2 tabs.

  2. Elucidating low-frequency vibrational dynamics in calcite and water with time-resolved third-harmonic generation spectroscopy.

    PubMed

    Wang, Liang; Liu, Weimin; Fang, Chong

    2015-07-14

    Low-frequency vibrations are foundational for material properties including thermal conductivity and chemical reactivity. To resolve the intrinsic molecular conformational dynamics in condensed phase, we implement time-resolved third-harmonic generation (TRTHG) spectroscopy to unravel collective skeletal motions in calcite, water, and aqueous salt solution in situ. The lifetime of three Raman-active modes in polycrystalline calcite at 155, 282 and 703 cm(-1) is found to be ca. 1.6 ps, 1.3 ps and 250 fs, respectively. The lifetime difference is due to crystallographic defects and anharmonic effects. By incorporating a home-built wire-guided liquid jet, we apply TRTHG to investigate pure water and ZnCl2 aqueous solution, revealing ultrafast dynamics of water intermolecular stretching and librational bands below 500 cm(-1) and a characteristic 280 cm(-1) vibrational mode in the ZnCl4(H2O)2(2-) complex. TRTHG proves to be a compact and versatile technique that directly uses the 800 nm fundamental laser pulse output to capture ultrafast low-frequency vibrational motion snapshots in condensed-phase materials including the omnipresent water, which provides the important time dimension to spectral characterization of molecular structure-function relationships. PMID:26062639

  3. Real-time Focused Ultrasound Surgery (FUS) Monitoring Using Harmonic Motion Imaging (HMI)

    SciTech Connect

    Maleke, Caroline; Konofagou, Elisa E.

    2009-04-14

    Monitoring changes in tissue mechanical properties to optimally control thermal exposure is important in thermal therapies. The amplitude-modulated (AM) harmonic motion imaging (HMI) for focused ultrasound (HMIFU) technique is a radiation force technique, which has the capability of tracking tissue stiffness during application of an oscillatory force. The feasibility of HMIFU for assessing mechanical tissue properties has been previously demonstrated. In this paper, a confocal transducer, combining a 4.5 MHz FUS transducer and a 3.3 MHz phased array imaging transducer, was used. The FUS transducer was driven by AM wave at 15 Hz with an acoustic intensity (I{sub spta}) was equal to 1050 W/cm{sup 2}. A lowpass digital filter was used to remove the spectrum of the higher power beam prior to displacement estimation. The resulting axial tissue displacement was estimated using 1D cross-correlation with a correlation window of 2 mm and a 92.5% overlap. A thermocouple was also used to measure the temperature near the ablated region. 2D HMI-images from six-bovine-liver specimens indicated the onset of coagulation necrosis through changes in amplitude displacement after coagulation due to its simultaneous probing and heating capability. The HMI technique can thus be used to monitor temperature-related stiffness changes of tissues during thermal therapies in real-time, i.e., without interrupting or modifying the treatment protocol.

  4. Quantitative evaluation of skeletal muscle defects in second harmonic generation images

    NASA Astrophysics Data System (ADS)

    Liu, Wenhua; Raben, Nina; Ralston, Evelyn

    2013-02-01

    Skeletal muscle pathologies cause irregularities in the normally periodic organization of the myofibrils. Objective grading of muscle morphology is necessary to assess muscle health, compare biopsies, and evaluate treatments and the evolution of disease. To facilitate such quantitation, we have developed a fast, sensitive, automatic imaging analysis software. It detects major and minor morphological changes by combining texture features and Fourier transform (FT) techniques. We apply this tool to second harmonic generation (SHG) images of muscle fibers which visualize the repeating myosin bands. Texture features are then calculated by using a Haralick gray-level cooccurrence matrix in MATLAB. Two scores are retrieved from the texture correlation plot by using FT and curve-fitting methods. The sensitivity of the technique was tested on SHG images of human adult and infant muscle biopsies and of mouse muscle samples. The scores are strongly correlated to muscle fiber condition. We named the software MARS (muscle assessment and rating scores). It is executed automatically and is highly sensitive even to subtle defects. We propose MARS as a powerful and unbiased tool to assess muscle health.

  5. Phase and Texture Characterizations of Scar Collagen Second-Harmonic Generation Images Varied with Scar Duration.

    PubMed

    Chen, Guannan; Liu, Yao; Zhu, Xiaoqin; Huang, Zufang; Cai, Jianyong; Chen, Rong; Xiong, Shuyuan; Zeng, Haishan

    2015-08-01

    This work developed a phase congruency algorithm combined with texture analysis to quantitatively characterize collagen morphology in second-harmonic generation (SHG) images from human scars. The extracted phase and texture parameters of the SHG images quantified collagen directionality, homogeneity, and coarseness in scars and varied with scar duration. Phase parameters showed an increasing tendency of the mean of phase congruency with scar duration, indicating that collagen fibers are better oriented over time. Texture parameters calculated from local difference local binary pattern (LD-LBP) and Haar wavelet transform, demonstrated that the LD-LBP variance decreased and the energy of all subimages increased with scar duration. It implied that collagen has a more regular pattern and becomes coarser with scar duration. In addition, the random forest regression was used to predict scar duration, demonstrating reliable performance of the extracted phase and texture parameters in characterizing collagen morphology in scar SHG images. Results indicate that the extracted parameters using the proposed method can be used as quantitative indicators to monitor scar progression with time and can help understand the mechanism of scar progression. PMID:26036282

  6. Optimization of contrast-to-tissue ratio through pulse windowing in dual-frequency “acoustic angiography” imaging

    PubMed Central

    Lindsey, Brooks D.; Shelton, Sarah E.; Dayton, Paul A.

    2016-01-01

    Early-stage tumors in many cancers are characterized by vascular remodeling, indicative of transformations in cell function. We have previously presented a high-resolution ultrasound imaging approach for detecting these changes which is based on microbubble contrast agents. In this technique, images are formed from only the higher harmonics of microbubble contrast agents, producing images of vasculature alone with 100–200 μm resolution. In this article, shaped transmit pulses are applied to imaging the higher broadband harmonic echoes of microbubble contrast agents, and the effects of varying pulse window and phasing on microbubble and tissue harmonic echoes are evaluated using a dual-frequency transducer in vitro and in vivo. An increase in contrast-to-tissue ratio of 6.8 ± 2.3 dB was observed in vitro by using an inverted pulse with a cosine window relative to a non-inverted pulse with a rectangular window. The increase in mean image intensity due to contrast enhancement in vivo in five rodents was 13.9 ± 3.0 dB greater for an inverted cosine-windowed pulse and 17.8 ± 3.6 dB greater for a non-inverted Gaussian-windowed relative to a non-inverted pulse with a rectangular window. Implications for pre-clinical and diagnostic imaging are also discussed. PMID:25819467

  7. Computationally Efficient Steady-State Solution of the Bloch Equations for Rapid Sinusoidal Scans Based on Fourier Expansion in Harmonics of the Scan Frequency

    PubMed Central

    Tseitlin, Mark; Eaton, Gareth R.; Eaton, Sandra S.

    2014-01-01

    Rapid-scan EPR has been shown to improve the signal-to-noise ratio relative to conventional continuous wave spectroscopy. Equations are derived for the steady-state solution to the Bloch equations as a Fourier expansion in the harmonics of the scan frequency. This simulation method is about two orders of magnitude faster than time-domain numerical integration. PMID:24678142

  8. A Novel Ku-Band/Ka-Band and Ka-Band/E-Band Multimode Waveguide Couplers for Power Measurement of Traveling-Wave Tube Amplifier Harmonic Frequencies

    NASA Technical Reports Server (NTRS)

    Wintucky, Edwin G.; Simons, Rainee N.

    2015-01-01

    This paper presents the design, fabrication and test results for a novel waveguide multimode directional coupler (MDC). The coupler, fabricated from two dissimilar frequency band waveguides, is capable of isolating power at the second harmonic frequency from the fundamental power at the output port of a traveling-wave tube (TWT) amplifier. Test results from proof-of-concept demonstrations are presented for a Ku-band/Ka-band MDC and a Ka-band/E-band MDC. In addition to power measurements at harmonic frequencies, a potential application of the MDC is in the design of a satellite borne beacon source for atmospheric propagation studies at millimeter-wave (mm-wave) frequencies (Ka-band and E-band).

  9. The Calculation of Accurate Harmonic Frequencies of Large Molecules: The Polycyclic Aromatic Hydrocarbons, a Case Study

    NASA Technical Reports Server (NTRS)

    Bauschlicher, Charles W., Jr.; Langhoff, Stephen R.; Arnold, James O. (Technical Monitor)

    1996-01-01

    The vibrational frequencies and infrared intensities of naphthalene neutral and cation are studied at the self-consistent-field (SCF), second-order Moller-Plesset (MP2), and density functional theory (DFT) levels using a variety of one-particle basis sets. Very accurate frequencies can be obtained at the DFT level in conjunction with large basis sets if they are scaled with two factors, one for the C-H stretches and a second for all other modes. We also find remarkably good agreement at the B3LYP/4-31G level using only one scale factor. Unlike the neutral PAHs where all methods do reasonably well for the intensities, only the DFT results are accurate for the PAH cations. The failure of the SCF and MP2 methods is caused by symmetry breaking and an inability to describe charge delocalization. We present several interesting cases of symmetry breaking in this study. An assessment is made as to whether an ensemble of PAH neutrals or cations could account for the unidentified infrared bands observed in many astronomical sources.

  10. Observations of high-frequency harmonic tremor in Fogo, Cape Verde Islands

    NASA Astrophysics Data System (ADS)

    Heleno, Sandra I. N.; Faria, Bruno V. E.; Bandomo, Zuleyka; Fonseca, João F. B. D.

    2006-11-01

    We report observations of high-frequency (HF) volcanic tremor, with spectral peaks in the band 5-20 Hz, recorded in Fogo Island, Cape Verde archipelago. Several occurrences are characterized by regularly spaced spectral peaks, but the fundamental frequency is missing. The signal is strong enough to be weakly detected on the southern islands of the archipelago, at distances of 20, 80 and 120 km from Fogo. This widespread detection of the HF tremor suggests that the propagation of the energy is made as T-waves propagating in the ocean sound channel. After correcting for site effects, the spatial pattern of amplitudes in Fogo and Brava Islands clearly indicates an offshore source. Active seamounts recently discovered in the vicinity of Fogo and Brava Islands [Hansteen, T.H., Grevemeyer, I., Hanel, K., Kraus, G., Schneider, J., Masson, D.G., Le Bas, T., Faria, B., 2006. Seamounts at the Cape Verde Islands: the geosphere-hydrosphere-biosphere connection, Seamount Biogeosciences Network, Workshop volume, La Jolla, March 24-25, 2006, 27-29.] are proposed to be the source of the HF tremor.

  11. Harmonic Nanoparticles for Regenerative Research

    PubMed Central

    Ronzoni, Flavio; Magouroux, Thibaud; Vernet, Remi; Extermann, Jérôme; Crotty, Darragh; Prina-Mello, Adriele; Ciepielewski, Daniel; Volkov, Yuri; Bonacina, Luigi; Wolf, Jean-Pierre; Jaconi, Marisa

    2014-01-01

    In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation nanoparticles (HNPs). The latter are a new family of probes recently introduced for labeling biological samples for multi-photon imaging. HNPs are capable of doubling the frequency of excitation light by the nonlinear optical process of second harmonic generation with no restriction on the excitation wavelength. Multi-photon based methodologies for hESC differentiation into cardiac clusters (maintained as long term air-liquid cultures) are presented in detail. In particular, evidence on how to maximize the intense second harmonic (SH) emission of isolated HNPs during 3D monitoring of beating cardiac tissue in 3D is shown. The analysis of the resulting images to retrieve 3D displacement patterns is also detailed. PMID:24836220

  12. Harmonic nanoparticles for regenerative research.

    PubMed

    Ronzoni, Flavio; Magouroux, Thibaud; Vernet, Remi; Extermann, Jérôme; Crotty, Darragh; Prina-Mello, Adriele; Ciepielewski, Daniel; Volkov, Yuri; Bonacina, Luigi; Wolf, Jean-Pierre; Jaconi, Marisa

    2014-01-01

    In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation nanoparticles (HNPs). The latter are a new family of probes recently introduced for labeling biological samples for multi-photon imaging. HNPs are capable of doubling the frequency of excitation light by the nonlinear optical process of second harmonic generation with no restriction on the excitation wavelength. Multi-photon based methodologies for hESC differentiation into cardiac clusters (maintained as long term air-liquid cultures) are presented in detail. In particular, evidence on how to maximize the intense second harmonic (SH) emission of isolated HNPs during 3D monitoring of beating cardiac tissue in 3D is shown. The analysis of the resulting images to retrieve 3D displacement patterns is also detailed. PMID:24836220

  13. Prediction and measurement of low-frequency harmonic noise of a hovering model helicopter rotor

    NASA Technical Reports Server (NTRS)

    Aggarawal, H. R.; Schmitz, F. H.; Boxwell, D. A.

    1989-01-01

    Far-field acoustic data for a model helicopter rotor have been gathered in a large open-jet, acoustically treated wind tunnel with the rotor operating in hover and out of ground-effect. The four-bladed Boeing 360 model rotor with advanced airfoils, planform, and tip shape was run over a range of conditions typical of today's modern helicopter main rotor. Near in-plane acoustic measurements were compared with two independent implementations of classical linear theory. Measured steady thrust and torque were used together with a free-wake analysis (to predict the thrust and drag distributions along the rotor radius) as input to this first-principles theoretical approach. Good agreement between theory and experiment was shown for both amplitude and phase for measurements made in those positions that minimized distortion of the radiated acoustic signature at low-frequencies.

  14. Estimating selected low-flow frequency statistics and harmonic-mean flows for ungaged, unregulated streams in Indiana

    USGS Publications Warehouse

    Martin, Gary R.; Fowler, Kathleen K.; Arihood, Leslie D.

    2016-01-01

    Information on low-flow characteristics of streams is essential for the management of water resources. This report provides equations for estimating the 1-, 7-, and 30-day mean low flows for a recurrence interval of 10 years and the harmonic-mean flow at ungaged, unregulated stream sites in Indiana. These equations were developed using the low-flow statistics and basin characteristics for 108 continuous-record streamgages in Indiana with at least 10 years of daily mean streamflow data through the 2011 climate year (April 1 through March 31). The equations were developed in cooperation with the Indiana Department of Environmental Management.Regression techniques were used to develop the equations for estimating low-flow frequency statistics and the harmonic-mean flows on the basis of drainage-basin characteristics. A geographic information system was used to measure basin characteristics for selected streamgages. A final set of 25 basin characteristics measured at all the streamgages were evaluated to choose the best predictors of the low-flow statistics.Logistic-regression equations applicable statewide are presented for estimating the probability that selected low-flow frequency statistics equal zero. These equations use the explanatory variables total drainage area, average transmissivity of the full thickness of the unconsolidated deposits within 1,000 feet of the stream network, and latitude of the basin outlet. The percentage of the streamgage low-flow statistics correctly classified as zero or nonzero using the logistic-regression equations ranged from 86.1 to 88.9 percent.Generalized-least-squares regression equations applicable statewide for estimating nonzero low-flow frequency statistics use total drainage area, the average hydraulic conductivity of the top 70 feet of unconsolidated deposits, the slope of the basin, and the index of permeability and thickness of the Quaternary surficial sediments as explanatory variables. The average standard error of

  15. Near Field Imaging at Microwave and Millemeter Wave Frequencies

    SciTech Connect

    Sheen, David M.; McMakin, Douglas L.; Hall, Thomas E.

    2007-06-03

    Near field imaging at microwave and millimeter wave frequencies is useful for a wide variety of applications including concealed weapon detection, through-wall and inner-wall imaging, ground penetrating radar imaging, radar cross section analysis, and non-destructive evaluation of materials. A variety of novel imaging techniques have been developed for many of these applications at the Pacific Northwest National Laboratory (PNNL) . These techniques make use of wideband holographic wavefront reconstruction methods, and have been developed to optimize the image quality and resolution. This paper will summarize several of these techniques and show imaging results for several interesting application areas.

  16. Beat-Frequency/Microsphere Medical Ultrasonic Imaging

    NASA Technical Reports Server (NTRS)

    Yost, William T.; Cantrell, John H.; Pretlow, Robert A., III

    1995-01-01

    Medical ultrasonic imaging system designed to provide quantitative data on various flows of blood in chambers, blood vessels, muscles, and tissues of heart. Sensitive enough to yield readings on flows of blood in heart even when microspheres used as ultrasonic contrast agents injected far from heart and diluted by circulation of blood elsewhere in body.

  17. Holographic Radar Imaging Privacy Techniques Utilizing Dual-Frequency Implementation

    SciTech Connect

    McMakin, Douglas L.; Hall, Thomas E.; Sheen, David M.

    2008-04-18

    Over the last 15 years, the Pacific Northwest National Laboratory has performed significant research and development activities to enhance the state of the art of holographic radar imaging systems to be used at security checkpoints for screening people for concealed threats hidden under their garments. These enhancement activities included improvements to privacy techniques to remove human features and providing automatic detection of body-worn concealed threats. The enhanced privacy and detection methods used both physical and software imaging techniques. The physical imaging techniques included polarization-diversity illumination and reception, dual-frequency implementation, and high-frequency imaging at 60 GHz. Software imaging techniques to enhance the privacy of the person under surveillance included extracting concealed threat artifacts from the imagery to automatically detect the threat. This paper will focus on physical privacy techniques using dual-frequency implementation.

  18. Second harmonic generation imaging of skin wound healing and scarring in a rabbit ear model

    NASA Astrophysics Data System (ADS)

    Tang, Yiyan; Zhu, Xiaoqin; Xiong, Shuyuan; Chen, Jianxin

    2012-12-01

    Skin wound healing and scarring in rabbit ears was examined by second harmonic generation (SHG) microscopy. Rabbit ear wound model was created by punching from the ventral surface with removal of epidermis, dermis and perichondrium. The samples were collected weekly, and cut into 100 μm thickness sections for SHG imaging. SHG imaging system was operated at 810 nm, producing SHG signals at half the excitation wavelength 405 nm. A Plan-Neofluar objective (x40 and NA=0.75) was employed for focusing the excitation beam into tissue samples and was also used to collect the backscattered intrinsic SHG signals. Our results showed apparent difference in collagen content and microstructure at various wound healing and scarring time points. It suggested that SHG signals from collagen can serve as a good indicator for characterization of wound status. With the advancement on miniaturization, microscopy based on SHG will become a valuable tool for monitoring the wound healing and scarring in vivo, and help to guide the improvement of scar appearance with appropriate and subtle modulation during wound healing based on better understanding of scarring response mechanism.

  19. Second harmonic generation imaging as a potential tool for staging pregnancy and predicting preterm birth

    NASA Astrophysics Data System (ADS)

    Akins, Meredith L.; Luby-Phelps, Katherine; Mahendroo, Mala

    2010-03-01

    We use second harmonic generation (SHG) microscopy to assess changes in collagen structure of murine cervix during cervical remodeling of normal pregnancy and in a preterm birth model. Visual inspection of SHG images revealed substantial changes in collagen morphology throughout normal gestation. SHG images collected in both the forward and backward directions were analyzed quantitatively for changes in overall mean intensity, forward to backward intensity ratio, collagen fiber size, and porosity. Changes in mean SHG intensity and intensity ratio take place in early pregnancy, suggesting that submicroscopic changes in collagen fibril size and arrangement occur before macroscopic changes become evident. Fiber size progressively increased from early to late pregnancy, while pores between collagen fibers became larger and farther apart. Analysis of collagen features in premature cervical remodeling show that changes in collagen structure are dissimilar from normal remodeling. The ability to quantify multiple morphological features of collagen that characterize normal cervical remodeling and distinguish abnormal remodeling in preterm birth models supports future studies aimed at development of SHG endoscopic devices for clinical assessment of collagen changes during pregnancy in women and for predicting risk of preterm labor which occurs in 12.5% of all pregnancies.

  20. Label-free imaging of Schwann cell myelination by third harmonic generation microscopy.

    PubMed

    Lim, Hyungsik; Sharoukhov, Denis; Kassim, Imran; Zhang, Yanqing; Salzer, James L; Melendez-Vasquez, Carmen V

    2014-12-16

    Understanding the dynamic axon-glial cell interaction underlying myelination is hampered by the lack of suitable imaging techniques. Here we demonstrate third harmonic generation microscopy (THGM) for label-free imaging of myelinating Schwann cells in live culture and ex vivo and in vivo tissue. A 3D structure was acquired for a variety of compact and noncompact myelin domains, including juxtaparanodes, Schmidt-Lanterman incisures, and Cajal bands. Other subcellular features of Schwann cells that escape traditional optical microscopies were also visualized. We tested THGM for morphometry of compact myelin. Unlike current methods based on electron microscopy, g-ratio could be determined along an extended length of myelinated fiber in the physiological condition. The precision of THGM-based g-ratio estimation was corroborated in mouse models of hypomyelination. Finally, we demonstrated the feasibility of THGM to monitor morphological changes of myelin during postnatal development and degeneration. The outstanding capabilities of THGM may be useful for elucidation of the mechanism of myelin formation and pathogenesis. PMID:25453108

  1. In vivo wound healing diagnosis with second harmonic and fluorescence lifetime imaging.

    PubMed

    Deka, Gitanjal; Wu, Wei-Wen; Kao, Fu-Jen

    2013-06-01

    Skin wounds heal when a series of cell lineages are triggered, followed by collagen deposition, to reconstruct damaged tissues. This study evaluates the regeneration of collagen and change in cellular metabolic rate in vivo during wound healing in rats, with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy respectively. The metabolic rate of cells is reflected through the lifetime of the autofluorescence from the co-enzyme protein, reduced nicotinamide adenine dinucleotide, due to its change in the relative concentration of bound and free forms. A higher than normal cellular metabolic rate is observed during the first week of healing, which decreases gradually after eight days of wound formation. SHG signal intensity change indicates the net degradation of collagen during the inflammatory phase, and net regeneration begins on day five. Eventually, the quantity of collagen increases gradually to form a scar tissue as the final product. Importantly, this work demonstrates the feasibility of an in vivo imaging approach for a normal wound on rat skin, which has the potential to supplement the noninvasive clinical diagnosis of wounds. PMID:23264966

  2. In vivo wound healing diagnosis with second harmonic and fluorescence lifetime imaging.

    PubMed

    Deka, Gitanjal; Wu, Wei-Wen; Kao, Fu-Jen

    2013-06-01

    Skin wounds heal when a series of cell lineages are triggered, followed by collagen deposition, to reconstruct damaged tissues. This study evaluates the regeneration of collagen and change in cellular metabolic rate in vivo during wound healing in rats, with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy respectively. The metabolic rate of cells is reflected through the lifetime of the autofluorescence from the co-enzyme protein, reduced nicotinamide adenine dinucleotide, due to its change in the relative concentration of bound and free forms. A higher than normal cellular metabolic rate is observed during the first week of healing, which decreases gradually after eight days of wound formation. SHG signal intensity change indicates the net degradation of collagen during the inflammatory phase, and net regeneration begins on day five. Eventually, the quantity of collagen increases gradually to form a scar tissue as the final product. Importantly, this work demonstrates the feasibility of an in vivo imaging approach for a normal wound on rat skin, which has the potential to supplement the noninvasive clinical diagnosis of wounds. PMID:23748703

  3. Depth-sensitive subsurface imaging of polymer nanocomposites using second harmonic Kelvin probe force microscopy.

    PubMed

    Castañeda-Uribe, Octavio Alejandro; Reifenberger, Ronald; Raman, Arvind; Avila, Alba

    2015-03-24

    We study the depth sensitivity and spatial resolution of subsurface imaging of polymer nanocomposites using second harmonic mapping in Kelvin Probe Force Microscopy (KPFM). This method allows the visualization of the clustering and percolation of buried Single Walled Carbon Nanotubes (SWCNTs) via capacitance gradient (∂C/∂z) maps. We develop a multilayered sample where thin layers of neat Polyimide (PI) (∼80 nm per layer) are sequentially spin-coated on well-dispersed SWCNT/Polyimide (PI) nanocomposite films. The multilayer nanocomposite system allows the acquisition of ∂C/∂z images of three-dimensional percolating networks of SWCNTs at different depths in the same region of the sample. We detect CNTs at a depth of ∼430 nm, and notice that the spatial resolution progressively deteriorates with increasing depth of the buried CNTs. Computational trends of ∂C/∂z vs CNT depth correlate the sensitivity and depth resolution with field penetration and spreading, and enable a possible approach to three-dimensional subsurface structure reconstruction. The results open the door to nondestructive, three-dimensional tomography and nanometrology techniques for nanocomposite applications. PMID:25591106

  4. In vivo wound healing diagnosis with second harmonic and fluorescence lifetime imaging

    NASA Astrophysics Data System (ADS)

    Deka, Gitanjal; Wu, Wei-Wen; Kao, Fu-Jen

    2013-06-01

    Skin wounds heal when a series of cell lineages are triggered, followed by collagen deposition, to reconstruct damaged tissues. This study evaluates the regeneration of collagen and change in cellular metabolic rate in vivo during wound healing in rats, with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy respectively. The metabolic rate of cells is reflected through the lifetime of the autofluorescence from the co-enzyme protein, reduced nicotinamide adenine dinucleotide, due to its change in the relative concentration of bound and free forms. A higher than normal cellular metabolic rate is observed during the first week of healing, which decreases gradually after eight days of wound formation. SHG signal intensity change indicates the net degradation of collagen during the inflammatory phase, and net regeneration begins on day five. Eventually, the quantity of collagen increases gradually to form a scar tissue as the final product. Importantly, this work demonstrates the feasibility of an in vivo imaging approach for a normal wound on rat skin, which has the potential to supplement the noninvasive clinical diagnosis of wounds.

  5. Nonlinear optical microscopy in biology: Combining second-harmonic generation and two-photon fluorescence imaging

    NASA Astrophysics Data System (ADS)

    Clays, Koen

    2011-03-01

    Optical microscopy has been since long a truly enabling visualization technique in the biological and biomedical sciences. Linear optical microscopy relies on simple linear optical effects. Nonlinear optical microscopy relies on the nonlinear optical properties of endogenous or exogenous chromophores to produce a better image. Two-photon fluorescence (TPF), a third-order nonlinear optical effect and observed at the focal spot only due to the quadratic intensity dependence, results in inherently higher resolution than possible for one-photon fluorescence, observed over the complete Rayleigh range. Second-harmonic generation (SHG) is a second-order nonlinear optical effect only observed for non-centrosymmetric arrangements of non-centrosymmetric chromophores. While this does put a restriction on the chromophores that can be used, it also results in structural information about symmetry when used in combination with TPF. TPF, being a third-order nonlinear process, is not restricted by any symmetry consideration. We will review the molecular design criteria for exogenous probes for combined SHG and TPF nonlinear microscopy, provide examples of optimized chromophores and show microscopy images demonstrating the use of such chromophores in nonlinear microscopy.

  6. A theory for the radiation at the third to fifth harmonics of the plasma frequency upstream from the earth's bow shock

    NASA Technical Reports Server (NTRS)

    Cairns, Iver H.

    1988-01-01

    A theory involving the process L + T' - T is proposed for the observed third to fifth harmonics of the plasma frequency observed upstream from the earth's bow shock. The conditions for which the theory may explain the observed radiation are determined, and the viability of the theory is discussed. The theory is found to require L waves with temperature between about 10 to the 21st and 10 to the 22nd K in the source regions to account for the levels and number of harmonics of the observed radiation. Such L wave temperatures are theoretically possible in the foreshock, but are not consistent with the most intense observed L waves.

  7. Ultra high frequency imaging acoustic microscope

    DOEpatents

    Deason, Vance A.; Telschow, Kenneth L.

    2006-05-23

    An imaging system includes: an object wavefront source and an optical microscope objective all positioned to direct an object wavefront onto an area of a vibrating subject surface encompassed by a field of view of the microscope objective, and to direct a modulated object wavefront reflected from the encompassed surface area through a photorefractive material; and a reference wavefront source and at least one phase modulator all positioned to direct a reference wavefront through the phase modulator and to direct a modulated reference wavefront from the phase modulator through the photorefractive material to interfere with the modulated object wavefront. The photorefractive material has a composition and a position such that interference of the modulated object wavefront and modulated reference wavefront occurs within the photorefractive material, providing a full-field, real-time image signal of the encompassed surface area.

  8. Towards more accurate numerical modeling of impedance based high frequency harmonic vibration

    NASA Astrophysics Data System (ADS)

    Lim, Yee Yan; Kiong Soh, Chee

    2014-03-01

    The application of smart materials in various fields of engineering has recently become increasingly popular. For instance, the high frequency based electromechanical impedance (EMI) technique employing smart piezoelectric materials is found to be versatile in structural health monitoring (SHM). Thus far, considerable efforts have been made to study and improve the technique. Various theoretical models of the EMI technique have been proposed in an attempt to better understand its behavior. So far, the three-dimensional (3D) coupled field finite element (FE) model has proved to be the most accurate. However, large discrepancies between the results of the FE model and experimental tests, especially in terms of the slope and magnitude of the admittance signatures, continue to exist and are yet to be resolved. This paper presents a series of parametric studies using the 3D coupled field finite element method (FEM) on all properties of materials involved in the lead zirconate titanate (PZT) structure interaction of the EMI technique, to investigate their effect on the admittance signatures acquired. FE model updating is then performed by adjusting the parameters to match the experimental results. One of the main reasons for the lower accuracy, especially in terms of magnitude and slope, of previous FE models is the difficulty in determining the damping related coefficients and the stiffness of the bonding layer. In this study, using the hysteretic damping model in place of Rayleigh damping, which is used by most researchers in this field, and updated bonding stiffness, an improved and more accurate FE model is achieved. The results of this paper are expected to be useful for future study of the subject area in terms of research and application, such as modeling, design and optimization.

  9. Image enhancement by adjusting the contrast of spatial frequencies

    NASA Astrophysics Data System (ADS)

    Yang, Ching-Chung

    2008-02-01

    We demonstrate a brand-new method for image enhancement by adjusting the contrast of different spatial frequencies. Fine characteristics of an image are well enhanced with negligible side effects. This method is easy to implement owing to its simple optical basis.

  10. Far-field imaging with a multi-frequency metalens

    SciTech Connect

    Jouvaud, C. Ourir, A.; Rosny, J. de

    2014-06-16

    A metalens, i.e., a dense array of identical resonators, allows to image an object pattern at subwavelength scale from far field radiation field. Here, we show that the efficiency can be improved when the resonant frequencies of the cell are distributed over a given frequency range. Because in such systems each eigen mode is localized, the subwavelength image is built from a spectral analysis of the radiated field. A simple model based on coupled resonant dipoles is used to find the best frequency distribution. This multifrequency metalens approach is validated using a flat array of split ring resonators. We experimentally demonstrate the subwavelength resolution of such a device at microwave range.

  11. What interactions can distort the orientational distribution of interfacial water molecules as probed by second harmonic and sum frequency generation?

    NASA Astrophysics Data System (ADS)

    de Beer, Alex G. F.; Roke, Sylvie

    2016-07-01

    Aqueous interfaces are omnipresent in nature. Nonlinear optical methods such as second harmonic and sum frequency generation (SHG/SFG) are valuable techniques to access molecular level information from these interfaces. In the interpretation of SHG and SFG data for both scattering and reflection mode experiments, the relation between the second-order hyperpolarizability tensor β(2), a molecular property, and the surface second-order susceptibility (" separators=" χ(2), a surface averaged property, plays a central role. To correctly describe the molecular details of the interface, it needs to be determined how molecules are oriented, and what the influence is of interfacial electrostatic fields and H-bonding on the orientational distribution. Here, we revisit the relations between β(2) and χ(2) and show, by means of a Boltzmann average, that significant energy differences are needed to generate measurable changes in the molecular orientational distribution at the interface. In practice, H-bonding and surface pressure such as applied in a Langmuir trough can be strong enough to alter the shape of the orientational distribution function of water. In contrast, electrostatic fields, such as those present in the Stern layer, will not have a significant impact on the shape of the orientational distribution function of water molecules.

  12. What interactions can distort the orientational distribution of interfacial water molecules as probed by second harmonic and sum frequency generation?

    PubMed

    de Beer, Alex G F; Roke, Sylvie

    2016-07-28

    Aqueous interfaces are omnipresent in nature. Nonlinear optical methods such as second harmonic and sum frequency generation (SHG/SFG) are valuable techniques to access molecular level information from these interfaces. In the interpretation of SHG and SFG data for both scattering and reflection mode experiments, the relation between the second-order hyperpolarizability tensor β(2), a molecular property, and the surface second-order susceptibility χ(2), a surface averaged property, plays a central role. To correctly describe the molecular details of the interface, it needs to be determined how molecules are oriented, and what the influence is of interfacial electrostatic fields and H-bonding on the orientational distribution. Here, we revisit the relations between β(2) and χ(2) and show, by means of a Boltzmann average, that significant energy differences are needed to generate measurable changes in the molecular orientational distribution at the interface. In practice, H-bonding and surface pressure such as applied in a Langmuir trough can be strong enough to alter the shape of the orientational distribution function of water. In contrast, electrostatic fields, such as those present in the Stern layer, will not have a significant impact on the shape of the orientational distribution function of water molecules. PMID:27475384

  13. Extracting Cardiac Myofiber Orientations from High Frequency Ultrasound Images.

    PubMed

    Qin, Xulei; Cong, Zhibin; Jiang, Rong; Shen, Ming; Wagner, Mary B; Kishbom, Paul; Fei, Baowei

    2013-03-29

    Cardiac myofiber plays an important role in stress mechanism during heart beating periods. The orientation of myofibers decides the effects of the stress distribution and the whole heart deformation. It is important to image and quantitatively extract these orientations for understanding the cardiac physiological and pathological mechanism and for diagnosis of chronic diseases. Ultrasound has been wildly used in cardiac diagnosis because of its ability of performing dynamic and noninvasive imaging and because of its low cost. An extraction method is proposed to automatically detect the cardiac myofiber orientations from high frequency ultrasound images. First, heart walls containing myofibers are imaged by B-mode high frequency (>20 MHz) ultrasound imaging. Second, myofiber orientations are extracted from ultrasound images using the proposed method that combines a nonlinear anisotropic diffusion filter, Canny edge detector, Hough transform, and K-means clustering. This method is validated by the results of ultrasound data from phantoms and pig hearts. PMID:24392208

  14. Extracting cardiac myofiber orientations from high frequency ultrasound images

    NASA Astrophysics Data System (ADS)

    Qin, Xulei; Cong, Zhibin; Jiang, Rong; Shen, Ming; Wagner, Mary B.; Kirshbom, Paul; Fei, Baowei

    2013-03-01

    Cardiac myofiber plays an important role in stress mechanism during heart beating periods. The orientation of myofibers decides the effects of the stress distribution and the whole heart deformation. It is important to image and quantitatively extract these orientations for understanding the cardiac physiological and pathological mechanism and for diagnosis of chronic diseases. Ultrasound has been wildly used in cardiac diagnosis because of its ability of performing dynamic and noninvasive imaging and because of its low cost. An extraction method is proposed to automatically detect the cardiac myofiber orientations from high frequency ultrasound images. First, heart walls containing myofibers are imaged by B-mode high frequency (<20 MHz) ultrasound imaging. Second, myofiber orientations are extracted from ultrasound images using the proposed method that combines a nonlinear anisotropic diffusion filter, Canny edge detector, Hough transform, and K-means clustering. This method is validated by the results of ultrasound data from phantoms and pig hearts.

  15. Third harmonic frequency generation by type-I critically phase-matched LiB3O5 crystal by means of optically active quartz crystal.

    PubMed

    Gapontsev, Valentin P; Tyrtyshnyy, Valentin A; Vershinin, Oleg I; Davydov, Boris L; Oulianov, Dmitri A

    2013-02-11

    We present a method of third harmonic generation at 355 nm by frequency mixing of fundamental and second harmonic radiation of an ytterbium nanosecond pulsed all-fiber laser in a type-I phase-matched LiB(3)O(5) (LBO) crystal where originally orthogonal polarization planes of the fundamental and second harmonic beams are aligned by an optically active quartz crystal. 8 W of ultraviolet light at 355 nm were achieved with 40% conversion efficiency from 1064 nm radiation. The conversion efficiency obtained in a type-I phase-matched LBO THG crystal was 1.6 times higher than the one achieved in a type-II LBO crystal at similar experimental conditions. In comparison to half-wave plates traditionally used for polarization alignment the optically active quartz crystal has much lower temperature dependence and requires simpler optical alignment. PMID:23481827

  16. Magnetic resonance imaging at frequencies below 1 kHz.

    PubMed

    Hilschenz, Ingo; Körber, Rainer; Scheer, Hans-Jürgen; Fedele, Tommaso; Albrecht, Hans-Helge; Mario Cassará, Antonino; Hartwig, Stefan; Trahms, Lutz; Haase, Jürgen; Burghoff, Martin

    2013-02-01

    Within the magnetic resonance imaging (MRI) community the trend is going to higher and higher magnetic fields, ranging from 1.5 T to 7 T, corresponding to Larmor frequencies of 63.8-298 MHz. Since for high-field MRI the magnetization increases with the applied magnetic field, the signal-to-noise-ratio increases as well, thus enabling higher image resolutions. On the other hand, MRI is possible also at ultra-low magnetic fields, as was shown by different groups. The goal of our development was to reach a Larmor frequency range of the low-field MRI system corresponding to the frequency range of human brain activities ranging from near zero-frequency (near-DC) to over 1 kHz. Here, first 2D MRI images of phantoms taken at Larmor frequencies of 100 Hz and 731 Hz will be shown and discussed. These frequencies are examples of brain activity triggered by electrostimulation of the median nerve. The method will allow the magnetic fields of the brain currents to influence the magnetic resonance image, and thus lead to a direct functional imaging modality of neuronal currents. PMID:22898690

  17. Detail enhancement of blurred infrared images based on frequency extrapolation

    NASA Astrophysics Data System (ADS)

    Xu, Fuyuan; Zeng, Deguo; Zhang, Jun; Zheng, Ziyang; Wei, Fei; Wang, Tiedan

    2016-05-01

    A novel algorithm for enhancing the details of the blurred infrared images based on frequency extrapolation has been raised in this paper. Unlike other researchers' work, this algorithm mainly focuses on how to predict the higher frequency information based on the Laplacian pyramid separation of the blurred image. This algorithm uses the first level of the high frequency component of the pyramid of the blurred image to reverse-generate a higher, non-existing frequency component, and adds back to the histogram equalized input blurred image. A simple nonlinear operator is used to analyze the extracted first level high frequency component of the pyramid. Two critical parameters are participated in the calculation known as the clipping parameter C and the scaling parameter S. The detailed analysis of how these two parameters work during the procedure is figure demonstrated in this paper. The blurred image will become clear, and the detail will be enhanced due to the added higher frequency information. This algorithm has the advantages of computational simplicity and great performance, and it can definitely be deployed in the real-time industrial applications. We have done lots of experiments and gave illustrations of the algorithm's performance in this paper to convince its effectiveness.

  18. Frequency-difference electrical impedance tomography: Phantom imaging experiments

    NASA Astrophysics Data System (ADS)

    Ahn, Sujin; Jun, Sung Chan; Seo, Jin Keun; Lee, Jeehyun; Woo, Eung Je; Holder, David

    2010-04-01

    Frequency-difference electrical impedance tomography (fdEIT) using a weighted voltage difference has been proposed as a means to provide images of admittivity changes at different frequencies. This weighted difference method is an effective way to extract anomaly information while eliminating background effects by unknown boundary geometry, uncertainty in electrode positions and other systematic measurement artefacts. It also properly handles the interplay between conductivity and permittivity in measured boundary voltage data. Though the proposed fdEIT algorithm is promising for applications such as detection of hemorrhagic stroke and breast cancer, more validation studies are needed. In this paper, we performed two-and three-dimensional numerical simulations and phantom experiments. Backgrounds of imaging objects were either saline or carrot pieces suspended in saline. We used carrot pieces to simulate a more realistic frequency-dependent admittivity distribution. Test objects were banana, potato or conductive gel with known admittivity spectra. When the background was saline, both simple and weighted difference approaches produced reasonably accurate images. The weighted difference method yielded better images from two-dimensional imaging objects with background of carrot pieces. For the three-dimensional head-shaped phantom, the advantage of the weighted frequency difference method over the simple difference method is not as obvious as in the case of the two-dimensional phantom. It is unclear if this is due to measurement errors or limitations in the linear algorithm. Further refinement and validation of the frequency difference image reconstructions are currently in progress.

  19. Analytical Bistatic k Space Images Compared to Experimental Swept Frequency EAR Images

    NASA Technical Reports Server (NTRS)

    Shaeffer, John; Cooper, Brett; Hom, Kam

    2004-01-01

    A case study of flat plate scattering images obtained by the analytical bistatic k space and experimental swept frequency ISAR methods is presented. The key advantage of the bistatic k space image is that a single excitation is required, i.e., one frequency I one angle. This means that prediction approaches such as MOM only need to compute one solution at a single frequency. Bistatic image Fourier transform data are obtained by computing the scattered field at various bistatic positions about the body in k space. Experimental image Fourier transform data are obtained from the measured response to a bandwidth of frequencies over a target rotation range.

  20. A Theory of Dynamic Imaging of Coherent Molecular Rotations by High Harmonic Generation

    NASA Astrophysics Data System (ADS)

    Faisal, F. H. M.; Abdurrouf, A.

    A dynamic theory of mapping coherent molecular motions from high harmonic generation signals is presented. Application to mapping of coherent rotational motions of linear molecules is made. Results of concrete calculations for N2, O2 and CO2 are analyzed both in time and frequency domains. A "magic angle" for the polarization geometry is predicted at which the HHG signals for all pump-probe delay times become equal for linear molecules of σg orbital symmetry. In contrast only a "crossing neighborhood" near the magic angle is predicted for molecules with orbitals of π symmetry. They are expected to help in identifying the orbital symmetry in the inverse problem of orbital reconstruction from experimental data. Comparison with available experimental data show remarkable agreement with all the salient properties of dynamic HHG signals of linear diatomic molecules, N2 and O2, and some simple organic molecules. Additional results for the triatomic molecule CO2 are discussed that might help to test the theory further.

  1. Real-time Monitoring of High Intensity Focused Ultrasound (HIFU) Ablation of In Vitro Canine Livers Using Harmonic Motion Imaging for Focused Ultrasound (HMIFU).

    PubMed

    Grondin, Julien; Payen, Thomas; Wang, Shutao; Konofagou, Elisa E

    2015-01-01

    Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a technique that can perform and monitor high-intensity focused ultrasound (HIFU) ablation. An oscillatory motion is generated at the focus of a 93-element and 4.5 MHz center frequency HIFU transducer by applying a 25 Hz amplitude-modulated signal using a function generator. A 64-element and 2.5 MHz imaging transducer with 68kPa peak pressure is confocally placed at the center of the HIFU transducer to acquire the radio-frequency (RF) channel data. In this protocol, real-time monitoring of thermal ablation using HIFU with an acoustic power of 7 W on canine livers in vitro is described. HIFU treatment is applied on the tissue during 2 min and the ablated region is imaged in real-time using diverging or plane wave imaging up to 1,000 frames/second. The matrix of RF channel data is multiplied by a sparse matrix for image reconstruction. The reconstructed field of view is of 90° for diverging wave and 20 mm for plane wave imaging and the data are sampled at 80 MHz. The reconstruction is performed on a Graphical Processing Unit (GPU) in order to image in real-time at a 4.5 display frame rate. 1-D normalized cross-correlation of the reconstructed RF data is used to estimate axial displacements in the focal region. The magnitude of the peak-to-peak displacement at the focal depth decreases during the thermal ablation which denotes stiffening of the tissue due to the formation of a lesion. The displacement signal-to-noise ratio (SNRd) at the focal area for plane wave was 1.4 times higher than for diverging wave showing that plane wave imaging appears to produce better displacement maps quality for HMIFU than diverging wave imaging. PMID:26556647

  2. Time dependent Doppler shifts in high-order harmonic generation in intense laser interactions with solid density plasma and frequency chirped pulses

    SciTech Connect

    Welch, E. C.; Zhang, P.; He, Z.-H.; Dollar, F.; Krushelnick, K.; Thomas, A. G. R.

    2015-05-15

    High order harmonic generation from solid targets is a compelling route to generating intense attosecond or even zeptosecond pulses. However, the effects of ion motion on the generation of harmonics have only recently started to be considered. Here, we study the effects of ion motion in harmonics production at ultrahigh laser intensities interacting with solid density plasma. Using particle-in-cell simulations, we find that there is an optimum density for harmonic production that depends on laser intensity, which scales linearly with a{sub 0} with no ion motion but with a reduced scaling if ion motion is included. We derive a scaling for this optimum density with ion motion and also find that the background ion motion induces Doppler red-shifts in the harmonic structures of the reflected pulse. The temporal structure of the Doppler shifts is correlated to the envelope of the incident laser pulse. We demonstrate that by introducing a frequency chirp in the incident pulse we are able to eliminate these Doppler shifts almost completely.

  3. Low frequency radio synthesis imaging of the galactic center region

    NASA Astrophysics Data System (ADS)

    Nord, Michael Evans

    2005-11-01

    The Very Large Array radio interferometer has been equipped with new receivers to allow observations at 330 and 74 MHz, frequencies much lower than were previously possible with this instrument. Though the VLA dishes are not optimal for working at these frequencies, the system is successful and regular observations are now taken at these frequencies. However, new data analysis techniques are required to work at these frequencies. The technique of self- calibration, used to remove small atmospheric effects at higher frequencies, has been adapted to compensate for ionospheric turbulence in much the same way that adaptive optics is used in the optical regime. Faceted imaging techniques are required to compensate for the noncoplanar image distortion that affects the system due to the wide fields of view at these frequencies (~2.3° at 330 MHz and ~11° at 74 MHz). Furthermore, radio frequency interference is a much larger problem at these frequencies than in higher frequencies and novel approaches to its mitigation are required. These new techniques and new system are allowing for imaging of the radio sky at sensitivities and resolutions orders of magnitude higher than were possible with the low frequency systems of decades past. In this work I discuss the advancements in low frequency data techniques required to make high resolution, high sensitivity, large field of view measurements with the new Very Large Array low frequency system and then detail the results of turning this new system and techniques on the center of our Milky Way Galaxy. At 330 MHz I image the Galactic center region with roughly 10 inches resolution and 1.6 mJy beam -1 sensitivity. New Galactic center nonthermal filaments, new pulsar candidates, and the lowest frequency detection to date of the radio source associated with our Galaxy's central massive black hole result. At 74 MHz I image a region of the sky roughly 40° x 6° with, ~10 feet resolution. I use the high opacity of H II regions at 74

  4. Integration of the denoising, inpainting and local harmonic B(z) algorithm for MREIT imaging of intact animals.

    PubMed

    Jeon, Kiwan; Kim, Hyung Joong; Lee, Chang-Ock; Seo, Jin Keun; Woo, Eung Je

    2010-12-21

    Conductivity imaging based on the current-injection MRI technique has been developed in magnetic resonance electrical impedance tomography. Current injected through a pair of surface electrodes induces a magnetic flux density distribution inside an imaging object, which results in additional magnetic field inhomogeneity. We can extract phase changes related to the current injection and obtain an image of the induced magnetic flux density. Without rotating the object inside the bore, we can measure only one component B(z) of the magnetic flux density B = (B(x), B(y), B(z)). Based on a relation between the internal conductivity distribution and B(z) data subject to multiple current injections, one may reconstruct cross-sectional conductivity images. As the image reconstruction algorithm, we have been using the harmonic B(z) algorithm in numerous experimental studies. Performing conductivity imaging of intact animal and human subjects, we found technical difficulties that originated from the MR signal void phenomena in the local regions of bones, lungs and gas-filled tubular organs. Measured B(z) data inside such a problematic region contain an excessive amount of noise that deteriorates the conductivity image quality. In order to alleviate this technical problem, we applied hybrid methods incorporating ramp-preserving denoising, harmonic inpainting with isotropic diffusion and ROI imaging using the local harmonic B(z) algorithm. These methods allow us to produce conductivity images of intact animals with best achievable quality. We suggest guidelines to choose a hybrid method depending on the overall noise level and existence of distinct problematic regions of MR signal void. PMID:21098914

  5. High Intensity Focused Ultrasound (HIFU) Focal Spot Localization Using Harmonic Motion Imaging (HMI)

    PubMed Central

    Han, Yang; Hou, Gary Yi; Wang, Shutao; Konofagou, Elisa

    2015-01-01

    Several ultrasound-based imaging modalities have been proposed for image guidance and monitoring of High-Intensity Focused Ultrasound (HIFU) treatment. However, accurate localization and characterization of the effective region of treatment (focal spot) remain important obstacles in the clinical implementation of HIFU ablation. Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a HIFU monitoring technique that utilizes radiation-force-induced localized oscillatory displacement. HMIFU has been shown to correctly identify the formation and extent of HIFU thermal ablation lesions. However a significant problem remains in identifying the location of the HIFU focus, which is necessary for treatment planning. In this study, the induced displacement was employed to localize the HIFU focal spot inside the tissue prior to treatment. Feasibility was shown with two separate systems. The 1D HMIFU system consisted of a HIFU transducer emitting an amplitude-modulated HIFU beam for mechanical excitation and a confocal single-element, pulse-echo transducer for simultaneous RF acquisition. The 2D HIFU system consists of a HIFU phased array, and a co-axial imaging phased array for simultaneous imaging. Initial feasibility was first performed on tissue-mimicking gelatin phantoms and the focal zone was defined as the region corresponding to the −3 dB full width at half maximum of the HMI displacement. Using the same parameters, in vitro experiments were performed in canine liver specimens to compare the defined focal zone with the lesion. In vitro measurements showed good agreement between the HMI predicted focal zone and the induced HIFU lesion location. HMIFU was experimentally shown to be capable of predicting and tracking the focal region in both phantoms and in vitro tissues. The accuracy of focal spot localization was evaluated by comparing with the lesion location in post-ablative tissues, with a R2 = 0.821 at p<0.002 in the 2D HMI system. We demonstrated the

  6. High intensity focused ultrasound (HIFU) focal spot localization using harmonic motion imaging (HMI)

    NASA Astrophysics Data System (ADS)

    Han, Yang; Hou, Gary Yi; Wang, Shutao; Konofagou, Elisa

    2015-08-01

    Several ultrasound-based imaging modalities have been proposed for image guidance and monitoring of high-intensity focused ultrasound (HIFU) treatment. However, accurate localization and characterization of the effective region of treatment (focal spot) remain important obstacles in the clinical implementation of HIFU ablation. Harmonic motion imaging for focused ultrasound (HMIFU) is a HIFU monitoring technique that utilizes radiation-force-induced localized oscillatory displacement. HMIFU has been shown to correctly identify the formation and extent of HIFU thermal ablation lesions. However a significant problem remains in identifying the location of the HIFU focus, which is necessary for treatment planning. In this study, the induced displacement was employed to localize the HIFU focal spot inside the tissue prior to treatment. Feasibility was shown with two separate systems. The 1D HMIFU system consisted of a HIFU transducer emitting an amplitude-modulated HIFU beam for mechanical excitation and a confocal single-element, pulse-echo transducer for simultaneous RF acquisition. The 2D HIFU system consists of a HIFU phased array, and a co-axial imaging phased array for simultaneous imaging. Initial feasibility was first performed on tissue-mimicking gelatin phantoms and the focal zone was defined as the region corresponding to the  -3dB full width at half maximum of the HMI displacement. Using the same parameters, in vitro experiments were performed in canine liver specimens to compare the defined focal zone with the lesion. In vitro measurements showed good agreement between the HMI predicted focal zone and the induced HIFU lesion location. HMIFU was experimentally shown to be capable of predicting and tracking the focal region in both phantoms and in vitro tissues. The accuracy of focal spot localization was evaluated by comparing with the lesion location in post-ablative tissues, with a R2 = 0.821 at p  <  0.002 in the 2D HMI system. We demonstrated

  7. Detecting subtle plasma membrane perturbation in living cells using second harmonic generation imaging.

    PubMed

    Moen, Erick K; Ibey, Bennett L; Beier, Hope T

    2014-05-20

    The requirement of center asymmetry for the creation of second harmonic generation (SHG) signals makes it an attractive technique for visualizing changes in interfacial layers such as the plasma membrane of biological cells. In this article, we explore the use of lipophilic SHG probes to detect minute perturbations in the plasma membrane. Three candidate probes, Di-4-ANEPPDHQ (Di-4), FM4-64, and all-trans-retinol, were evaluated for SHG effectiveness in Jurkat cells. Di-4 proved superior with both strong SHG signal and limited bleaching artifacts. To test whether rapid changes in membrane symmetry could be detected using SHG, we exposed cells to nanosecond-pulsed electric fields, which are believed to cause formation of nanopores in the plasma membrane. Upon nanosecond-pulsed electric fields exposure, we observed an instantaneous drop of ~50% in SHG signal from the anodic pole of the cell. When compared to the simultaneously acquired fluorescence signals, it appears that the signal change was not due to the probe diffusing out of the membrane or changes in membrane potential or fluidity. We hypothesize that this loss in SHG signal is due to disruption in the interfacial nature of the membrane. The results show that SHG imaging has great potential as a tool for measuring rapid and subtle plasma membrane disturbance in living cells. PMID:24853757

  8. Evidence for Harmonic Content and Frequency Evolution of Oscillations During the Rising Phase of X-ray Bursts From 4U 1636-536

    NASA Technical Reports Server (NTRS)

    Bgattacharyya, Sudip; Strohmayer, E.

    2005-01-01

    We report on a study of the evolution of burst oscillation properties during the rising phase of X-ray bursts from 4U 1636-536 observed with the proportional counter array (PCA) on board the Rossi X-Ray Timing Explorer (RXTE) . We present evidence for significant harmonic structure of burst oscillation pulses during the early rising phases of bursts. This is the first such detection in burst rise oscillations, and is very important for constraining neutron star structure parameters and the equation of state models of matter at the core of a neutron star. The detection of harmonic content only during the initial portions of the burst rise is consistent with the theoretical expectation that with time the thermonuclear burning region becomes larger, and hence the fundamental and harmonic amplitudes both diminish. We also find, for the first time from this source, strong evidence of oscillation frequency increase during the burst rise. The timing behavior of harmonic content, amplitude, and frequency of burst rise oscillations may be important in understanding the spreading of thermonuclear flames under the extreme physical conditions on neutron star surfaces.

  9. Effect of bandwidth on beam smoothing and frequency conversion at the third harmonic of the Nova laser

    SciTech Connect

    Pennington, D.M.; Henesian, M.A.; Dixit, S.N.; Powell, H.T.; Thompson, C.E.; Weiland, T.L.

    1993-05-01

    We present the results of experiments performed on the Nova laser system to determine the effect of bandwidth on third harmonic (3{omega}) frequency conversion and beam smoothing by spectral dispersion (SSD). Our experiments utilized a wide bandwidth fiber optic cross-phase modulated (XPM) source and a narrower bandwidth microwave modulated (FM) source, each centered at 1053 nm (1{omega}). The FM source produced {approximately}2 cm{sup {minus}1} of bandwidth, modulated at 3 GHz; the XPM bandwidth was varied from 5 to 15 cm{sup {minus}1}, modulated by the temporally noisy output of a multimode Nd:glass laser ({le} 500 GHz). The FM beam showed no evidence of self-phase modulation in the laser chain produced by intensity fluctuations, and 1{omega} bandwidth was tripled upon conversion to 3{omega} (2--6 cm{sup {minus}1}). The 1{omega} XPM bandwidth increased by {ge} 25% due to self-phase modulation in the laser chain (16--22 cm{sup {minus}1}) due to it`s relative noisy temporal structure. Over 50% of the 1{omega} XPM bandwidth was transferred to the 3{omega} beam (22--36 cm{sup {minus}1}), yielding 0.13% bandwidth at 3{omega}. The maximum intrinsic narrowband 3{omega} frequency conversion obtained using a type-II/type-II KDP crystal array was 62%. The intrinsic efficiency obtained at the Nova 10-beam chamber is typically > 65%. We have developed broadband frequency conversion codes and broadband pulse simulations to model our results, and have obtained good agreement with experiment. Using a random phase plate without bandwidth, we obtained a smoothing level, {sigma}/I {approximately} 0.79, defined by the rms variance normalized with respect to the average intensity. This is less than the theoretically expected value of 1 for an ideal speckle pattern, and could be evidence of polarization smoothing as a result of focus lens birefringence. With spectral dispersion and RPP we demonstrated an excellent level of smoothing with the XPM source.

  10. Clinical skin imaging using color spatial frequency domain imaging (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Yang, Bin; Lesicko, John; Moy, Austin J.; Reichenberg, Jason; Tunnell, James W.

    2016-02-01

    Skin diseases are typically associated with underlying biochemical and structural changes compared with normal tissues, which alter the optical properties of the skin lesions, such as tissue absorption and scattering. Although widely used in dermatology clinics, conventional dermatoscopes don't have the ability to selectively image tissue absorption and scattering, which may limit its diagnostic power. Here we report a novel clinical skin imaging technique called color spatial frequency domain imaging (cSFDI) which enhances contrast by rendering color spatial frequency domain (SFD) image at high spatial frequency. Moreover, by tuning spatial frequency, we can obtain both absorption weighted and scattering weighted images. We developed a handheld imaging system specifically for clinical skin imaging. The flexible configuration of the system allows for better access to skin lesions in hard-to-reach regions. A total of 48 lesions from 31 patients were imaged under 470nm, 530nm and 655nm illumination at a spatial frequency of 0.6mm^(-1). The SFD reflectance images at 470nm, 530nm and 655nm were assigned to blue (B), green (G) and red (R) channels to render a color SFD image. Our results indicated that color SFD images at f=0.6mm-1 revealed properties that were not seen in standard color images. Structural features were enhanced and absorption features were reduced, which helped to identify the sources of the contrast. This imaging technique provides additional insights into skin lesions and may better assist clinical diagnosis.

  11. Synthetic aperture sonar imaging using joint time-frequency analysis

    NASA Astrophysics Data System (ADS)

    Wang, Genyuan; Xia, Xiang-Gen

    1999-03-01

    The non-ideal motion of the hydrophone usually induces the aperture error of the synthetic aperture sonar (SAS), which is one of the most important factors degrading the SAS imaging quality. In the SAS imaging, the return signals are usually nonstationary due to the non-ideal hydrophone motion. In this paper, joint time-frequency analysis (JTFA), as a good technique for analyzing nonstationary signals, is used in the SAS imaging. Based on the JTFA of the sonar return signals, a novel SAS imaging algorithm is proposed. The algorithm is verified by simulation examples.

  12. Frequency Domain Optical Coherence Tomography Techniques in Eye Imaging

    NASA Astrophysics Data System (ADS)

    Wojtkowski, M.; Kowalczyk, A.; Targowski, P.; Gorczyñska, I.

    2000-12-01

    This contribution presents an application of frequency-domain optical tomography to ophthalmology. Essential theoretical foundations of time-domain and frequency-domain optical tomography are presented. Images of sections through the anterior chamber, the corneo-scleral angle and fundus of the eye are reconstructed from the spectral fringes. The morphological information gained by tomograms is important for diagnosing and planning of a treatment of glaucoma.

  13. Ultrasound breast imaging using frequency domain reverse time migration

    NASA Astrophysics Data System (ADS)

    Roy, O.; Zuberi, M. A. H.; Pratt, R. G.; Duric, N.

    2016-04-01

    Conventional ultrasonography reconstruction techniques, such as B-mode, are based on a simple wave propagation model derived from a high frequency approximation. Therefore, to minimize model mismatch, the central frequency of the input pulse is typically chosen between 3 and 15 megahertz. Despite the increase in theoretical resolution, operating at higher frequencies comes at the cost of lower signal-to-noise ratio. This ultimately degrades the image contrast and overall quality at higher imaging depths. To address this issue, we investigate a reflection imaging technique, known as reverse time migration, which uses a more accurate propagation model for reconstruction. We present preliminary simulation results as well as physical phantom image reconstructions obtained using data acquired with a breast imaging ultrasound tomography prototype. The original reconstructions are filtered to remove low-wavenumber artifacts that arise due to the inclusion of the direct arrivals. We demonstrate the advantage of using an accurate sound speed model in the reverse time migration process. We also explain how the increase in computational complexity can be mitigated using a frequency domain approach and a parallel computing platform.

  14. Coronal loops diagnostics using the parameters of U-burst harmonic pair at frequencies 10-70 MHz

    NASA Astrophysics Data System (ADS)

    Dorovskyy, V. V.; Melnik, V. N.; Konovalenko, A. A.; Bubnov, I. N.; Gridin, A. A.; Shevchuk, N. V.; Rucker, H. O.; Panchenko, M.

    2013-09-01

    The results of the first observations of solar sporadic radio emission using one section of the new being currently created Giant Ukrainian Radio Telescope (GURT) are presented. The parameters of inverted U-burst with harmonic structure observed with GURT are considered. The main attention is paid to the time delay between the fundamental and harmonic components. The analytical model explaining the observed time delay is proposed.

  15. Wideband image demodulation via bi-dimensional multirate frequency transformations.

    PubMed

    Liu, Wenjing; Santhanam, Balu

    2016-09-01

    Existing image demodulation approaches based on the two-dimensional (2D) multicomponent AM-FM model assume narrowband components that can be demodulated using energy operators, Hilbert transforms, or the monogenic image approaches. However, if the FM components are wideband, then these demodulation approaches incur significant errors. Recent work by the authors extended wideband FM demodulation in one dimension to accommodate large conversion factors using multirate frequency transformations. In this paper, we extend the multirate frequency transformations technique developed for one-dimensional signals to 2D and images in conjunction with a recently proposed 2D higher-order energy demodulation approach. This extension is applied to both synthetic and real images to demonstrate the efficacy of the approach. PMID:27607487

  16. Walk-Off-Induced Modulation Instability, Temporal Pattern Formation, and Frequency Comb Generation in Cavity-Enhanced Second-Harmonic Generation

    NASA Astrophysics Data System (ADS)

    Leo, F.; Hansson, T.; Ricciardi, I.; De Rosa, M.; Coen, S.; Wabnitz, S.; Erkintalo, M.

    2016-01-01

    We derive a time-domain mean-field equation to model the full temporal and spectral dynamics of light in singly resonant cavity-enhanced second-harmonic generation systems. We show that the temporal walk-off between the fundamental and the second-harmonic fields plays a decisive role under realistic conditions, giving rise to rich, previously unidentified nonlinear behavior. Through linear stability analysis and numerical simulations, we discover a new kind of quadratic modulation instability which leads to the formation of optical frequency combs and associated time-domain dissipative structures. Our numerical simulations show excellent agreement with recent experimental observations of frequency combs in quadratic nonlinear media [Phys. Rev. A 91, 063839 (2015)]. Thus, in addition to unveiling a new, experimentally accessible regime of nonlinear dynamics, our work enables predictive modeling of frequency comb generation in cavity-enhanced second-harmonic generation systems. We expect our findings to have wide impact on the study of temporal and spectral dynamics in a diverse range of dispersive, quadratically nonlinear resonators.

  17. Experimental investigation of the ionospheric hysteresis effect on the threshold excitation level of the Stimulated Electromagnetic Emission (SEE) during heating at the second electron gyro-harmonic frequency

    NASA Astrophysics Data System (ADS)

    Samimi, A.; Scales, W.; Cruz, M.; Isham, B.; Bernhardt, P. A.

    2012-12-01

    Recent experimental observations of the stimulated electromagnetic emission (SEE) spectrum during heating at the second electron gyro-harmonic show structures ordered by ion gyro-frequency. The proposed generation mechanism considers parametric decay of a pump upper hybrid/electron Bernstein (UH/EB) wave into another UH/EB and a group of neutralized ion Bernstein waves. The presumption of the proposed mechanism is that the pump electromagnetic wave is converted into the UH/EB wave. This conversion process generates field aligned irregularity which exhibits hysteresis effect. The predicted ionospheric hysteresis effect is studied during the PARS 2012 at HAARP. The preliminary results are presented for the first time. Also, experimental study of the effects of 1) the transmitter beam angle and 2) the transmitter frequency offset relative to the second electron gyro-harmonic frequency on the ion gyro-harmonic structures in the SEE spectrum are provided. The aforementioned observations are compared to the predictions of the analytical model. Possible connection of the SEE spectral features and artificially generated ionospheric descending layer is also discussed

  18. Electromagnetic MUSIC-type imaging of perfectly conducting, arc-like cracks at single frequency

    NASA Astrophysics Data System (ADS)

    Park, Won-Kwang; Lesselier, Dominique

    2009-11-01

    We propose a non-iterative MUSIC (MUltiple SIgnal Classification)-type algorithm for the time-harmonic electromagnetic imaging of one or more perfectly conducting, arc-like cracks found within a homogeneous space R2. The algorithm is based on a factorization of the Multi-Static Response (MSR) matrix collected in the far-field at a single, nonzero frequency in either Transverse Magnetic (TM) mode (Dirichlet boundary condition) or Transverse Electric (TE) mode (Neumann boundary condition), followed by the calculation of a MUSIC cost functional expected to exhibit peaks along the crack curves each half a wavelength. Numerical experimentation from exact, noiseless and noisy data shows that this is indeed the case and that the proposed algorithm behaves in robust manner, with better results in the TM mode than in the TE mode for which one would have to estimate the normal to the crack to get the most optimal results.

  19. Exchange and polarization effect in high-order harmonic imaging of molecular structures

    SciTech Connect

    Sukiasyan, Suren; Ivanov, Misha Yu.; Patchkovskii, Serguei; Smirnova, Olga; Brabec, Thomas

    2010-10-15

    We analyze the importance of exchange, polarization, and electron-electron correlation in high-order harmonic generation in molecules interacting with intense laser fields. We find that electron exchange can become particularly important for harmonic emission associated with intermediate excitations in the molecular ion. In particular, for orbitals associated with two-hole one-particle excitations, exchange effects can eliminate structure-related minima and maxima in the harmonic spectra. Laser-induced polarization of the neutral molecule may also have major effects on orbital structure-related minima and maxima in the harmonic spectra. Finally, we show how exchange terms in recombination can be viewed as a shakedownlike process induced by sudden electronic excitation in the ion.

  20. Stepped frequency imaging for flaw monitoring: Final report

    SciTech Connect

    Hildebrand, B.P.

    1988-09-01

    This report summarizes the results of research into the usefulness of stepped frequency imaging (SFI) to nuclear power plant inspection. SFI is a method for producing ultrasonic holographic images without the need to sweep a two-dimensional aperture with the transducer. Instead, the transducer may be translated along a line. At each position of the transducer the frequency is stepped over a finite preselected bandwidth. The frequency stepped data is then processed to synthesize the second dimension. In this way it is possible to generate images in regions that are relatively inaccessible to two-dimensional scanners. This report reviews the theory and experimental work verifying the technique, and then explores its possible applications in the nuclear power industry. It also outlines how this new capability can be incorporated into the SDL-1000 Imaging System previously developed for EPRI. The report concludes with five suggestions for uses for the SFI method. These are: monitoring suspect or repaired regions of feedwater nozzles; monitoring pipe cracks repaired by weld overlay; monitoring crack depth during test block production; imaging flaws where access is difficult; and imaging flaws through cladding without distortion.

  1. Polarization dependant in vivo second harmonic generation imaging of Caenorhabditis elegans vulval, pharynx, and body wall muscles

    NASA Astrophysics Data System (ADS)

    Psilodimitrakopoulos, Sotiris; Santos, Susana; Amat-Roldan, Ivan; Mathew, Manoj; Thayil K. N., Anisha; Artigas, David; Loza-Alvarez, Pablo

    2008-02-01

    Second harmonic generation (SHG) imaging has emerged in recent years as an important laboratory imaging technique since it can provide unique structural information with submicron resolution. It enjoys the benefits of non-invasive interaction establishing this imaging modality as ideal for in vivo investigation of tissue architectures. In this study we present, polarization dependant high resolution SHG images of Caenorhabditis elegans muscles in vivo. We imaged a variety of muscular structures such as body walls, pharynx and vulva. By fitting the experimental data into a cylindrical symmetry spatial model we mapped the corresponding signal distribution of the χ (2) tensor and identified its main axis orientation for different sarcomeres of the earth worm. The cylindrical symmetry was considered to arise from the thick filaments architecture of the inside active volume. Moreover, our theoretical analysis allowed calculating the mean orientation of harmonophores (myosin helical pitch). Ultimately, we recorded and analysed vulvae muscle dynamics, where SHG signal decreased during in vivo contraction.

  2. An ab initio study of the in-plane harmonic force fields and fundamental vibration frequencies of cis- and trans-1,3-butadiene

    NASA Astrophysics Data System (ADS)

    Bock, Charles W.; Trachtman, Mendel; George, Philip

    1980-11-01

    The structure, in-plane force field, and fundamental vibration frequencies of trans- and cis-1,3-butadiene are calculated ab initio using the 4-31G basis set. Using a scaling procedure based on computational results from smaller molecules, the vibration frequencies for the trans-conformer calculated from the ab initio force constant matrix are found on average to be within 2.2% of the experimental values "harmonized" according to Dennison's rule. The values predicted for the cis-conformer, for which experimental spectroscopic data are only now becoming available, should facilitate the complete in-plane assignment of fundamentals in the near future.

  3. Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

    NASA Astrophysics Data System (ADS)

    Raja, Anju M.; Xu, Shuoyu; Sun, Wanxin; Zhou, Jianbiao; Tai, Dean C. S.; Chen, Chien-Shing; Rajapakse, Jagath C.; So, Peter T. C.; Yu, Hanry

    2010-09-01

    Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.

  4. Electromagnetic induction imaging with a radio-frequency atomic magnetometer

    NASA Astrophysics Data System (ADS)

    Deans, Cameron; Marmugi, Luca; Hussain, Sarah; Renzoni, Ferruccio

    2016-03-01

    We report on a compact, tunable, and scalable to large arrays imaging device, based on a radio-frequency optically pumped atomic magnetometer operating in magnetic induction tomography modality. Imaging of conductive objects is performed at room temperature, in an unshielded environment and without background subtraction. Conductivity maps of target objects exhibit not only excellent performance in terms of shape reconstruction but also demonstrate detection of sub-millimetric cracks and penetration of conductive barriers. The results presented here demonstrate the potential of a future generation of imaging instruments, which combine magnetic induction tomography and the unmatched performance of atomic magnetometers.

  5. Polarized spatial frequency domain imaging of heart valve fiber structure

    NASA Astrophysics Data System (ADS)

    Goth, Will; Yang, Bin; Lesicko, John; Allen, Alicia; Sacks, Michael S.; Tunnell, James W.

    2016-03-01

    Our group previously introduced Polarized Spatial Frequency Domain Imaging (PSFDI), a wide-field, reflectance imaging technique which we used to empirically map fiber direction in porcine pulmonary heart valve leaflets (PHVL) without optical clearing or physical sectioning of the sample. Presented is an extended analysis of our PSFDI results using an inverse Mueller matrix model of polarized light scattering that allows additional maps of fiber orientation distribution, along with instrumentation permitting increased imaging speed for dynamic PHVL fiber measurements. We imaged electrospun fiber phantoms with PSFDI, and then compared these measurements to SEM data collected for the same phantoms. PHVL was then imaged and compared to results of the same leaflets optically cleared and imaged with small angle light scattering (SALS). The static PHVL images showed distinct regional variance of fiber orientation distribution, matching our SALS results. We used our improved imaging speed to observe bovine tendon subjected to dynamic loading using a biaxial stretching device. Our dynamic imaging experiment showed trackable changes in the fiber microstructure of biological tissue under loading. Our new PSFDI analysis model and instrumentation allows characterization of fiber structure within heart valve tissues (as validated with SALS measurements), along with imaging of dynamic fiber remodeling. The experimental data will be used as inputs to our constitutive models of PHVL tissue to fully characterize these tissues' elastic behavior, and has immediate application in determining the mechanisms of structural and functional failure in PHVLs used as bio-prosthetic implants.

  6. Harmonic engine

    DOEpatents

    Bennett, Charles L.

    2009-10-20

    A high efficiency harmonic engine based on a resonantly reciprocating piston expander that extracts work from heat and pressurizes working fluid in a reciprocating piston compressor. The engine preferably includes harmonic oscillator valves capable of oscillating at a resonant frequency for controlling the flow of working fluid into and out of the expander, and also preferably includes a shunt line connecting an expansion chamber of the expander to a buffer chamber of the expander for minimizing pressure variations in the fluidic circuit of the engine. The engine is especially designed to operate with very high temperature input to the expander and very low temperature input to the compressor, to produce very high thermal conversion efficiency.

  7. Multi-modal image matching based on local frequency information

    NASA Astrophysics Data System (ADS)

    Liu, Xiaochun; Lei, Zhihui; Yu, Qifeng; Zhang, Xiaohu; Shang, Yang; Hou, Wang

    2013-12-01

    This paper challenges the issue of matching between multi-modal images with similar physical structures but different appearances. To emphasize the common structural information while suppressing the illumination and sensor-dependent information between multi-modal images, two image representations namely Mean Local Phase Angle (MLPA) and Frequency Spread Phase Congruency (FSPC) are proposed by using local frequency information in Log-Gabor wavelet transformation space. A confidence-aided similarity (CAS) that consists of a confidence component and a similarity component is designed to establish the correspondence between multi-modal images. The two representations are both invariant to contrast reversal and non-homogeneous illumination variation, and without any derivative or thresholding operation. The CAS that integrates MLPA with FSPC tightly instead of treating them separately can more weight the common structures emphasized by FSPC, and therefore further eliminate the influence of different sensor properties. We demonstrate the accuracy and robustness of our method by comparing it with those popular methods of multi-modal image matching. Experimental results show that our method improves the traditional multi-modal image matching, and can work robustly even in quite challenging situations (e.g. SAR & optical image).

  8. High frequency ultrasound imaging in pupillary block glaucoma.

    PubMed Central

    Aslanides, I M; Libre, P E; Silverman, R H; Reinstein, D Z; Lazzaro, D R; Rondeau, M J; Harmon, G K; Coleman, D J

    1995-01-01

    BACKGROUND--The diagnosis of pupillary block glaucoma requires sufficient clarity of the ocular media. This is particularly important for assessment of both the presence and patency of an iridotomy, and the determination of central anterior chamber depth. METHODS--High frequency ultrasonography was used in three patients with suspected pupillary block to determine iris configuration, posterior chamber volume, and ciliary body conformation. RESULTS--All patients demonstrated high frequency ultrasonographic findings consistent with pupillary block: iris bombé, a formed posterior chamber, and a lack of anterior rotation of the ciliary processes. CONCLUSION--High frequency ultrasound imaging appears to be a valuable adjunct in making or corroborating the diagnosis of pupillary block glaucoma. Images PMID:8534666

  9. Spectroscopic imaging of buried layers in 2+1D via tabletop ptychography with high-harmonic EUV illumination

    NASA Astrophysics Data System (ADS)

    Gardner, Dennis F.; Porter, Christina L.; Shanblatt, Elisabeth R.; Mancini, Giulia F.; Karl, Robert; Tanksalvala, Michael; Bevis, Charles; Kapteyn, Henry C.; Murnane, Margaret M.; Adams, Daniel E.

    2016-03-01

    We use EUV coherent microscopy to obtain high-resolution images of buried interfaces, with chemical specificity, in 2+1 dimensions. We perform reflection mode, ptychographic, coherent diffractive imaging with tabletop EUV light, at 29nm, produced by high harmonic generation. Our damascene-style samples consist of copper structures inlaid in SiO2, polished nearly flat with chemical mechanical polishing. We obtain images of both an unaltered damascene as well as one buried below a 100nm thick layer of evaporated aluminum. The aluminum is opaque to visible light and thick enough that neither optical microscopy, SEM, nor AFM can access the buried interface. EUV microscopy is able to image the buried structures, non-destructively, in conditions where other techniques cannot.

  10. Comparison of Shielding Effect of Steady State Coherent Synchrotron Radiation Using the Image Charge Method and Frequency Analysis

    NASA Astrophysics Data System (ADS)

    Li, R.; Bohn, C. L.; Bisognano, J. J.

    1997-05-01

    There have been several studies([1] J. S. Nodvick and D. S. Saxon, Phys. Rev.) 96, 180 (1954); [2] S. A. Kheifets and B. Zotter, CERN SL-95-92 (AP), 1995; [3] B. Murphy, S. Krinsky, and R. L. Gluckstern, Phys. Rev. E 35, 2584 (1996). on the shielding effect of the coherent synchrotron radiation (CSR) emitted by a Gaussian bunch on a circular orbit in the center plane between two parallel plates. A functional dependence of the beam and machine parameters was given in [2] using the asymptotic analysis in the frequency domain. It indicates the shielded CSR mainly arises from harmonics n_th < n < n_c, where the threshold harmonic is n_th = √2/3(π ρ /h)^3/2, and harmonic cutoff is nc = ρ/σ (ρ: bend radius, h: spacing between plates, σ_s: bunch rms length). In this paper, we extend the frequency domain analysis to the parameter regime n_th > n_c, and the result is compared with the steady state CSR power obtained using the image charge method.

  11. Effect of Frequency and Migration Aperture on Seismic Diffraction Imaging

    NASA Astrophysics Data System (ADS)

    Bashir, Y.; Ghosh, D. P.; Moussavi Alashloo, S. Y.; Sum, C. W.

    2016-02-01

    Conventional processing and migration frequently give successful results in using specular reflections to estimate the subsurface geometry and strength of continuous reflector geology. However, the correct interpretation of the true geological gaps, such as fault, fracture, karsts and pinch-outs, is one of the main objectives in seismic data processing and interpretation. In regular processing/migration sequence the diffraction response is suppressed because of the lack of choosing the right migration aperture. Kirchhoff migration is a tool to represent the seismic data as a summation of diffraction hyperbolas governed by the velocities at their apex. In this paper, we have investigated two different velocity models to show the effects of different frequencies and aperture size. We used the diffraction-based and data oriented approach that is dependent on the migration aperture from a low to high aperture to properly image the section. We have done the error analysis between the un-imaged and imaged section after processing and observed that the low aperture can give the undesired result for sharp edges. For the same model, we have applied different frequencies to show the effect of frequencies on seismic Imaging and migration.

  12. Three frequency false-color image of Prince Albert, Canada

    NASA Technical Reports Server (NTRS)

    1994-01-01

    This is a three-frequency, false color image of Prince Albert, Canada, centered at 53.91 north latitude and 104.69 west longitude. It was produced using data from the X-band, C-band and L-band radars that comprise the Spaceborne Imaging Radar-C/X-band Synthetic Aperture Radar (SIR-C/X-SAR). SIR-C/X-SAR acquired this image on the 20th orbit of the Shuttle Endeavour. The area is located 40 km north and 30 km east of the town of Prince Albert in the Saskatchewan province of Canada. The image covers the area east of the Candle Lake, between gravel surface highways 120 and 106 and west of 106. The area in the middle of the image covers the entire Nipawin (Narrow Hills) provincial park. Most of the dark blue areas in the image are the ice covered lakes. The dark area on the top right corner of the image is the White Gull Lake north of the intersection of highway 120 and 913. The right middle part of the image shows Lake Ispuchaw and Lower Fishing Lake. The deforested areas are shown by light

  13. Effects of frequency- and direction-dependent elastic materials on linearly elastic MRE image reconstructions

    PubMed Central

    Perreard, I M; Pattison, A J; Doyley, M; McGarry, M D J; Barani, Z; Van Houten, E E; Weaver, J B; Paulsen, K D

    2013-01-01

    The mechanical model commonly used in magnetic resonance elastography (MRE) is linear elasticity. However, soft tissue may exhibit frequency-and direction-dependent (FDD) shear moduli in response to an induced excitation causing a purely linear elastic model to provide an inaccurate image reconstruction of its mechanical properties. The goal of this study was to characterize the effects of reconstructing FDD data using a linear elastic inversion (LEI) algorithm. Linear and FDD phantoms were manufactured and LEI images were obtained from time-harmonic MRE acquisitions with variations in frequency and driving signal amplitude. LEI responses to artificially imposed uniform phase shifts in the displacement data from both purely linear elastic and FDD phantoms were also evaluated. Of the variety of FDD phantoms considered, LEI appeared to tolerate viscoelastic data-model mismatch better than deviations caused by poroelastic and anisotropic mechanical properties in terms of visual image contrast. However, the estimated shear modulus values were substantially incorrect relative to independent mechanical measurements even in the successful viscoelastic cases and the variations in mean values with changes in experimental conditions associated with uniform phase shifts, driving signal frequency and amplitude were unpredictable. Overall, use of LEI to reconstruct data acquired in phantoms with FDD material properties provided biased results under the best conditions and significant artifacts in the worst cases. These findings suggest that the success with which LEI is applied to MRE data in tissue will depend on the underlying mechanical characteristics of the tissues and/or organs systems of clinical interest. PMID:21030746

  14. Dynamic infrared imaging in identification of breast cancer tissue with combined image processing and frequency analysis.

    PubMed

    Joro, R; Lääperi, A-L; Soimakallio, S; Järvenpää, R; Kuukasjärvi, T; Toivonen, T; Saaristo, R; Dastidar, P

    2008-01-01

    Five combinations of image-processing algorithms were applied to dynamic infrared (IR) images of six breast cancer patients preoperatively to establish optimal enhancement of cancer tissue before frequency analysis. mid-wave photovoltaic (PV) IR cameras with 320x254 and 640x512 pixels were used. The signal-to-noise ratio and the specificity for breast cancer were evaluated with the image-processing combinations from the image series of each patient. Before image processing and frequency analysis the effect of patient movement was minimized with a stabilization program developed and tested in the study by stabilizing image slices using surface markers set as measurement points on the skin of the imaged breast. A mathematical equation for superiority value was developed for comparison of the key ratios of the image-processing combinations. The ability of each combination to locate the mammography finding of breast cancer in each patient was compared. Our results show that data collected with a 640x512-pixel mid-wave PV camera applying image-processing methods optimizing signal-to-noise ratio, morphological image processing and linear image restoration before frequency analysis possess the greatest superiority value, showing the cancer area most clearly also in the match centre of the mammography estimation. PMID:18666012

  15. High-speed polarization sensitive optical frequency domain imaging with frequency multiplexing

    PubMed Central

    Yun, S.H.; Vakoc, B.J.; Shishkov, M.; Desjardins, A.E.; Park, B.H.; de Boer, J.F.; Tearney, G.J.; Bouma, B.E.

    2009-01-01

    Polarization sensitive optical coherence tomography (PS-OCT) provides a cross-sectional image of birefringence in biological samples that is complementary in many applications to the standard reflectance-based image. Recent ex vivo studies have demonstrated that birefringence mapping enables the characterization of collagen and smooth muscle concentration and distribution in vascular tissues. Instruments capable of applying these measurements percutaneously in vivo may provide new insights into coronary atherosclerosis and acute myocardial infarction. We have developed a polarization sensitive optical frequency domain imaging (PS-OFDI) system that enables high-speed intravascular birefringence imaging through a fiber-optic catheter. The novel design of this system utilizes frequency multiplexing to simultaneously measure reflectance of two incident polarization states, overcoming concerns regarding temporal variations of the catheter fiber birefringence and spatial variations in the birefringence of the sample. We demonstrate circular cross-sectional birefringence imaging of a human coronary artery ex vivo through a flexible fiber-optic catheter with an A-line rate of 62 kHz and a ranging depth of 6.2 mm. PMID:18542183

  16. High-speed optical frequency-domain imaging

    PubMed Central

    Yun, S. H.; Tearney, G. J.; de Boer, J. F.; Iftimia, N.; Bouma, B. E.

    2009-01-01

    We demonstrate high-speed, high-sensitivity, high-resolution optical imaging based on optical frequency-domain interferometry using a rapidly-tuned wavelength-swept laser. We derive and show experimentally that frequency-domain ranging provides a superior signal-to-noise ratio compared with conventional time-domain ranging as used in optical coherence tomography. A high sensitivity of −110 dB was obtained with a 6 mW source at an axial resolution of 13.5 µm and an A-line rate of 15.7 kHz, representing more than an order-of-magnitude improvement compared with previous OCT and interferometric imaging methods. PMID:19471415

  17. Frequency Up-Conversion Photon-Type Terahertz Imager.

    PubMed

    Fu, Z L; Gu, L L; Guo, X G; Tan, Z Y; Wan, W J; Zhou, T; Shao, D X; Zhang, R; Cao, J C

    2016-01-01

    Terahertz imaging has many important potential applications. Due to the failure of Si readout integrated circuits (ROICs) and the thermal mismatch between the photo-detector arrays and the ROICs at temperatures below 40 K, there are big technical challenges to construct terahertz photo-type focal plane arrays. In this work, we report pixel-less photo-type terahertz imagers based on the frequency up-conversion technique. The devices are composed of terahertz quantum-well photo-detectors (QWPs) and near-infrared (NIR) light emitting diodes (LEDs) which are grown in sequence on the same substrates using molecular beam epitaxy. In such an integrated QWP-LED device, photocurrent in the QWP drives the LED to emit NIR light. By optimizing the structural parameters of the QWP-LED, the QWP part and the LED part both work well. The maximum values of the internal and external energy up-conversion efficiencies are around 20% and 0.5%. A laser spot of a homemade terahertz quantum cascade laser is imaged by the QWP-LED together with a commercial Si camera. The pixel-less imaging results show that the image blurring induced by the transverse spreading of photocurrent is negligible. The demonstrated pixel-less imaging opens a new way to realize high performance terahertz imaging devices. PMID:27147281

  18. Frequency Up-Conversion Photon-Type Terahertz Imager

    PubMed Central

    Fu, Z. L.; Gu, L. L.; Guo, X. G.; Tan, Z. Y.; Wan, W. J.; Zhou, T.; Shao, D. X.; Zhang, R.; Cao, J. C.

    2016-01-01

    Terahertz imaging has many important potential applications. Due to the failure of Si readout integrated circuits (ROICs) and the thermal mismatch between the photo-detector arrays and the ROICs at temperatures below 40 K, there are big technical challenges to construct terahertz photo-type focal plane arrays. In this work, we report pixel-less photo-type terahertz imagers based on the frequency up-conversion technique. The devices are composed of terahertz quantum-well photo-detectors (QWPs) and near-infrared (NIR) light emitting diodes (LEDs) which are grown in sequence on the same substrates using molecular beam epitaxy. In such an integrated QWP-LED device, photocurrent in the QWP drives the LED to emit NIR light. By optimizing the structural parameters of the QWP-LED, the QWP part and the LED part both work well. The maximum values of the internal and external energy up-conversion efficiencies are around 20% and 0.5%. A laser spot of a homemade terahertz quantum cascade laser is imaged by the QWP-LED together with a commercial Si camera. The pixel-less imaging results show that the image blurring induced by the transverse spreading of photocurrent is negligible. The demonstrated pixel-less imaging opens a new way to realize high performance terahertz imaging devices. PMID:27147281

  19. High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

    PubMed Central

    Weed, Scott A.

    2015-01-01

    High-frequency ultrasound (HFUS) is widely employed as a non-invasive method for imaging internal anatomic structures in experimental small animal systems. HFUS has the ability to detect structures as small as 30 µm, a property that has been utilized for visualizing superficial lymph nodes in rodents in brightness (B)-mode. Combining power Doppler with B-mode imaging allows for measuring circulatory blood flow within lymph nodes and other organs. While HFUS has been utilized for lymph node imaging in a number of mouse  model systems, a detailed protocol describing HFUS imaging and characterization of the cervical lymph nodes in mice has not been reported. Here, we show that HFUS can be adapted to detect and characterize cervical lymph nodes in mice. Combined B-mode and power Doppler imaging can be used to detect increases in blood flow in immunologically-enlarged cervical nodes. We also describe the use of B-mode imaging to conduct fine needle biopsies of cervical lymph nodes to retrieve lymph tissue for histological  analysis. Finally, software-aided steps are described to calculate changes in lymph node volume and to visualize changes in lymph node morphology following image reconstruction. The ability to visually monitor changes in cervical lymph node biology over time provides a simple and powerful technique for the non-invasive monitoring of cervical lymph node alterations in preclinical mouse models of oral cavity disease. PMID:26274059

  20. Frequency Up-Conversion Photon-Type Terahertz Imager

    NASA Astrophysics Data System (ADS)

    Fu, Z. L.; Gu, L. L.; Guo, X. G.; Tan, Z. Y.; Wan, W. J.; Zhou, T.; Shao, D. X.; Zhang, R.; Cao, J. C.

    2016-05-01

    Terahertz imaging has many important potential applications. Due to the failure of Si readout integrated circuits (ROICs) and the thermal mismatch between the photo-detector arrays and the ROICs at temperatures below 40 K, there are big technical challenges to construct terahertz photo-type focal plane arrays. In this work, we report pixel-less photo-type terahertz imagers based on the frequency up-conversion technique. The devices are composed of terahertz quantum-well photo-detectors (QWPs) and near-infrared (NIR) light emitting diodes (LEDs) which are grown in sequence on the same substrates using molecular beam epitaxy. In such an integrated QWP-LED device, photocurrent in the QWP drives the LED to emit NIR light. By optimizing the structural parameters of the QWP-LED, the QWP part and the LED part both work well. The maximum values of the internal and external energy up-conversion efficiencies are around 20% and 0.5%. A laser spot of a homemade terahertz quantum cascade laser is imaged by the QWP-LED together with a commercial Si camera. The pixel-less imaging results show that the image blurring induced by the transverse spreading of photocurrent is negligible. The demonstrated pixel-less imaging opens a new way to realize high performance terahertz imaging devices.

  1. High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes.

    PubMed

    Walk, Elyse L; McLaughlin, Sarah L; Weed, Scott A

    2015-01-01

    High-frequency ultrasound (HFUS) is widely employed as a non-invasive method for imaging internal anatomic structures in experimental small animal systems. HFUS has the ability to detect structures as small as 30 µm, a property that has been utilized for visualizing superficial lymph nodes in rodents in brightness (B)-mode. Combining power Doppler with B-mode imaging allows for measuring circulatory blood flow within lymph nodes and other organs. While HFUS has been utilized for lymph node imaging in a number of mouse  model systems, a detailed protocol describing HFUS imaging and characterization of the cervical lymph nodes in mice has not been reported. Here, we show that HFUS can be adapted to detect and characterize cervical lymph nodes in mice. Combined B-mode and power Doppler imaging can be used to detect increases in blood flow in immunologically-enlarged cervical nodes. We also describe the use of B-mode imaging to conduct fine needle biopsies of cervical lymph nodes to retrieve lymph tissue for histological  analysis. Finally, software-aided steps are described to calculate changes in lymph node volume and to visualize changes in lymph node morphology following image reconstruction. The ability to visually monitor changes in cervical lymph node biology over time provides a simple and powerful technique for the non-invasive monitoring of cervical lymph node alterations in preclinical mouse models of oral cavity disease. PMID:26274059

  2. Performance assessment of HIFU lesion detection by Harmonic Motion Imaging for Focused Ultrasound (HMIFU): A 3D finite-element-based framework with experimental validation

    PubMed Central

    Hou, Gary Y.; Luo, Jianwen; Marquet, Fabrice; Maleke, Caroline; Vappou, Jonathan; Konofagou, Elisa E.

    2014-01-01

    Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a novel high-intensity focused ultrasound (HIFU) therapy monitoring method with feasibilities demonstrated in vitro, ex vivo and in vivo. Its principle is based on Amplitude-modulated (AM) - Harmonic Motion Imaging (HMI), an oscillatory radiation force used for imaging the tissue mechanical response during thermal ablation. In this study, a theoretical framework of HMIFU is presented, comprising a customized nonlinear wave propagation model, a finite-element (FE) analysis module, and an image-formation model. The objective of this study is to develop such a framework in order to 1) assess the fundamental performance of HMIFU in detecting HIFU lesions based on the change in tissue apparent elasticity, i.e., the increasing Young's modulus, and the HIFU lesion size with respect to the HIFU exposure time and 2) validate the simulation findings ex vivo. The same HMI and HMIFU parameters as in the experimental studies were used, i.e., 4.5-MHz HIFU frequency and 25 Hz AM frequency. For a lesion-to-background Young's modulus ratio of 3, 6, and 9, the FE and estimated HMI displacement ratios were equal to 1.83, 3.69, 5.39 and 1.65, 3.19, 4.59, respectively. In experiments, the HMI displacement followed a similar increasing trend of 1.19, 1.28, and 1.78 at 10-s, 20-s, and 30-s HIFU exposure, respectively. In addition, moderate agreement in lesion size growth was also found in both simulations (16.2, 73.1 and 334.7 mm2) and experiments (26.2, 94.2 and 206.2 mm2). Therefore, the feasibility of HMIFU for HIFU lesion detection based on the underlying tissue elasticity changes was verified through the developed theoretical framework, i.e., validation of the fundamental performance of the HMIFU system for lesion detection, localization and quantification, was demonstrated both theoretically and ex vivo. PMID:22036637

  3. Multispectral mid-infrared imaging using frequency upconversion

    NASA Astrophysics Data System (ADS)

    Sanders, Nicolai; Dam, Jeppe Seidelin; Jensen, Ole Bjarlin; Tidemand-Lichtenberg, Peter; Pedersen, Christian

    2013-03-01

    It has recently been shown that it is possible to upconvert infrared images to the near infrared region with high quantum efficiency and low noise by three-wave mixing with a laser field [1]. If the mixing laser is single-frequency, the upconverted image is simply a band-pass filtered version of the infrared object field, with a bandwidth corresponding given by the acceptance parameter of the conversion process, and a center frequency given by the phase-match condition. Tuning of the phase-matched wavelengths has previously been demonstrated by changing the temperature [2] or angle [3 Keywords: Infrared imaging, nonlinear frequency conversion, diode lasers, upconversion ] of the nonlinear material. Unfortunately, temperature tuning is slow, and angle tuning typically results in alignment issues. Here we present a novel approach where the wavelength of the mixing field is used as a tuning parameter, allowing for fast tuning and hence potentially fast image acquisition, paving the way for upconversion based real time multispectral imaging. In the present realization the upconversion module consists of an external cavity tapered diode laser in a Littrow configuration with a computer controlled feedback grating. The output from a tunable laser is used as seed for a fiber amplifier system, boosting the power to approx. 3 W over the tuning range from 1025 to 1085 nm. Using a periodically poled lithium niobate crystal, the infrared wavelength that can be phase-matched is tunable over more than 200 nm. Using a crystal with multiple poling periods allows for upconversion within the entire transparency range of the nonlinear material.

  4. Linewidth of the harmonics in a microwave frequency comb generated by focusing a mode-locked ultrafast laser on a tunneling junction

    SciTech Connect

    Hagmann, Mark J.; Stenger, Frank S.; Yarotski, Dmitry A.

    2013-12-14

    Previous analyses suggest that microwave frequency combs (MFCs) with harmonics having extremely narrow linewidths could be produced by photodetection with a mode-locked ultrafast laser. In the MFC generated by focusing a passively mode-locked ultrafast laser on a tunneling junction, 200 harmonics from 74.254 MHz to 14.85 GHz have reproducible measured linewidths approximating the 1 Hz resolution bandwidth (RBW) of the spectrum analyzer. However, in new measurements at a RBW of 0.1 Hz, the linewidths are distributed from 0.12 to 1.17 Hz. Measurements and analysis suggest that, because the laser is not stabilized, the stochastic drift in the pulse repetition rate is the cause for the distribution in measured linewidths. It appears that there are three cases in which the RBW is (1) greater than, (2) less than, or (3) comparable with the intrinsic linewidth. The measured spectra in the third class are stochastic and may show two or more peaks at a single harmonic.

  5. 1.5 W green light generation by single-pass second harmonic generation of a single-frequency tapered diode laser.

    PubMed

    Jensen, Ole Bjarlin; Andersen, Peter E; Sumpf, Bernd; Hasler, Karl-Heinz; Erbert, Götz; Petersen, Paul Michael

    2009-04-13

    More than 1.5 W of green light at 531 nm is generated by single-pass second harmonic generation in periodically poled MgO:LiNbO3. The pump laser is a high power tapered laser with a distributed Bragg reflector etched in the ridge section of the laser to provide wavelength selectivity. The output power of the single-frequency tapered laser is 9.3 W in continuous wave operation. A conversion efficiency of 18.5 % was achieved in the experiments. PMID:19365478

  6. Brillouin-zone integration schemes: an efficiency study for the phonon frequency moments of the harmonic, solid, one-component plasma

    SciTech Connect

    Albers, R.C.; Gubernatis, J.E.

    1981-01-01

    The efficiency of four different Brillouin-zone integration schemes including the uniform mesh, special point method, special directions method, and Holas method are compared for calculating moments of the harmonic phonon frequencies of the solid one-component plasma. Very accurate values for the moments are also presented. The Holas method for which weights and integration points can easily be generated has roughly the same efficiency as the special directions method, which is much superior to the uniform mesh and special point methods for this problem.

  7. Broadband wavelength converters with flattop responses based on cascaded second-harmonic generation and difference frequency generation in Bessel-chirped gratings.

    PubMed

    Liu, Tao; Djordjevic, Ivan B; Song, Zekun; Chen, Ying; Zhang, Rongxiang; Zhang, Ke; Zhao, Wei; Li, Baogang

    2016-05-16

    We investigate ultra-broadband wavelength converters based on cascaded second-harmonic generation and difference frequency generation using Bessel-chirped gratings (BCGs) in lithium niobate waveguides, and compare them to the ones using uniform grating and segmented grating, respectively. For the same length and power, the BCGs show broader bandwidth than the other two types of grating. The ripple of the matching response is very small as well. Analysis also shows that almost the same conversion bandwidth and maximum conversion efficiency with tolerant response flatness can be achieved when the manufacturing tolerance of the waveguide length is smaller than 0.1 cm. PMID:27409916

  8. Ion cyclotron range of frequency heating of a deuterium-tritium plasma via the second-harmonic tritium cyclotron resonance

    SciTech Connect

    Wilson, J.R.; Bush, C.E.; Darrow, D.; Hosea, J.C.; Jaeger, E.F.; Majeski, R.; Murakami, M.; Phillips, C.K.; Rogers, J.H.; Schilling, G.; Stevens, J.E.; Synakowski, E.; Taylor, G.

    1995-07-31

    Experiments have been performed on the TFTR to study rf wave heating of a D-T plasma by way of the second-harmonic tritium cyclotron resonance. The addition of tritium ions to a deuterium plasma allows for absorption of the rf waves at the tritium cyclotron harmonics and by electron damping of a mode converted ion Bernstein wave. Competing mechanisms include direct electron damping and damping at the fundamental cyclotron resonance of deuterium, {alpha} particles, and {sup 3}He ions. The contribution of each is estimated from a series of plasma discharges where various plasma parameters are varied. The majority of the rf power is found to damp on the tritium ions.

  9. Time and Space Resolved High Harmonic Imaging of Electron Tunnelling from Molecules

    NASA Astrophysics Data System (ADS)

    Smirnova, O.

    2009-05-01

    High harmonic generation in intense laser fields carries the promise of combining sub-Angstrom spatial and attosecond temporal resolution of electronic structures and dynamics in molecules, see e.g. [1-3]. High harmonic emission occurs when an electron detached from a molecule by an intense laser field recombines with the parent ion [4]. Similar to Young's double-slit experiment, recombination to several ``lobes'' of the same molecular orbital can produce interference minima and maxima in harmonic intensities [1]. These minima (maxima) carry structural information -- they occur when the de-Broglie wavelength of the recombining electron matches distances between the centers. We demonstrate both theoretically and experimentally that amplitude minima (maxima) in the harmonic spectra can also have dynamical origin, reflecting multi-electron dynamics in the molecule. We use high harmonic spectra to record this dynamics and reconstruct the position of the hole left in the molecule after ionization. Experimental data are consistent with the hole starting in different places as the ionization dynamics changes from tunnelling to the multi-photon regime. Importantly, hole localization and subsequent attosecond dynamics are induced even in the tunnelling limit. Thus, even ``static'' tunnelling induced by a tip of a tunnelling microscope will generate similar attosecond dynamics in a sample. We anticipate that our approach will become standard in disentangling spatial and temporal information from high harmonic spectra of molecules.[4pt] In collaboration with Serguei Patchkovskii, National Research Council, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada; Yann Mairesse, NRC Canada and CELIA, Universit'e Bordeaux I, UMR 5107 (CNRS, Bordeaux 1, CEA), 351 Cours de la Lib'eration, 33405 Talence Cedex, France; Nirit Dudovich, NRC Canada and Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel; David Villeneuve, Paul Corkum, NRC Canada

  10. Sum frequency generation imaging microscopy of CO on platinum.

    PubMed

    Cimatu, Katherine; Baldelli, Steven

    2006-12-20

    Sum frequency vibrational spectroscopy is utilized as an imaging technique to distinguish and compare the local response of carbon monoxide (CO) covered platinum (Pt) polycrystalline surface versus the average response of the investigated area. The Pt electrode was prepared using the standard method and was exposed to approximately 1 atm of CO(g). SFG images and vibrational spectra were obtained where the contrast is based on the intrinsic nature of each peak in the CO vibrational spectrum. The illustration of the images and the chemical maps of CO on the platinum surface showed the distribution of the CO across the observed area. The results obtained by comparing the local and the average response confirmed the spatial distributions of the CO on the platinum sample which are due to several reasons such as dipole-dipole coupling and surface coverage. This finding has a significant contribution toward recognizing that surfaces usually considered homogeneous may in fact be quite heterogeneous. PMID:17165737

  11. Applying Echoes Mean Frequency Shift for Attenuation Imaging in Tissue

    NASA Astrophysics Data System (ADS)

    Litniewski, J.; Klimonda, Z.; Nowicki, A.

    The purpose of this study was to develop the attenuation parametric imaging technique and to apply it for in vivo characterization of tissue. Local attenuation coefficient was determined by evaluating the frequency downshift that encounters the amplitude spectrum of the interrogating ultrasonic pulse during propagation in the absorbing tissue. Operation and accuracy of the processing methods were verified by assessing the size-independent region of interest (ROI) for attenuation determination and calculating the attenuation coefficient distribution for experimentally recorded tissue-phantom scattered waveforms. The Siemens Antares scanner equipped with Ultrasound Research Interface unit allowing for direct radiofrequency (RF) signals recording was used for B-scan imaging of the tissue- mimicking phantom in vitro and liver in vivo. RF data were processed to determine attenuation coefficient along the B-scan lines. Also, the preliminary studies of backscattered signals from skin recorded using a skin scanner were performed to calculate parametric-attenuation images.

  12. Three frequency false color image of Flevoland, the Netherlands

    NASA Technical Reports Server (NTRS)

    1994-01-01

    This is a three-frequency false color image of Flevoland, the Netherlands, centered at 52.4 degrees north latitude, 5.4 degrees east longitude. This image was acquired by the Spaceborne Imaging Radar-C and X-Band Synthetic Aperture Radar (SIR-C/X-SAR) aboard the Shuttle Endeavour. The area shown covers an area approximately 25 kilometers by 28 kilometers. Flevoland, which fills the lower two-thirds of the image, is a very flat area that is made up of reclaimed land that is used for agriculture and forestry. At the top of the image, across the canal from Flevoland, is an older forest shown in red; the city of Harderwijk is shown in white on the shore of the canal. At this time of the year, the agricultural fields are bare soil, and they show up in this image in blue. The dark blue areas are water and the small dots in the canal are boats. The Jet Propulsion Laboratory alternative photo number is P-43941.

  13. Design of variable frequency endoscope ultrasonic digital imaging system

    NASA Astrophysics Data System (ADS)

    Li, Ya-nan; Bai, Bao-ping; Chen, Xiao-dong; Zhao, Qiang; Deng, Hao-ran; Wang, Yi; Yu, Dao-yin

    2013-12-01

    This paper presented a real-time endoscope ultrasonic digital imaging system, which was based on FPGA and applied for gastrointestinal examination. Four modules, scan-line data processing module, coordinate transformation and interpolation algorithm module, cache reading and writing control module and transmitting and receiving control module were included in this FPGA based system. Through adopting different frequency ultrasound probes in a single insertion of endoscope, the system showed a high speed data processing mechanism capable of achieving images with various display effects. A high-precision modified coordinate calibration CORDIC (HMCC-CORDIC) algorithm was employed to realize coordinate transformation and interpolation simultaneously, while the precision and reliability of the algorithm could be greatly improved through utilizing the pipeline structure based on temporal logic. Also, system real-time control by computer could be achieved through operating under the condition of USB2.0 interface. The corresponding experimental validations proved the feasibility and the correctness of the proper data processing mechanism, the HMCC-CORDIC algorithm and the USB real-time control. Finally, the specific experimental sample, a tissue mimicking phantom, was imaged in real-time (25 frames per second) by an endoscope ultrasonic imaging system with image size 1024×1024. The requirements for clinical examination could be well satisfied with the imaging parameters discussed above.

  14. Concealed weapons detection using low-frequency magnetic imaging

    NASA Astrophysics Data System (ADS)

    Zollars, Byron G.; Sallee, Bradley; Durrett, Michael G.; Cruce, Clay; Hallidy, William

    1997-02-01

    Military personnel, law-enforcement officers, and civilians face ever-increasing dangers from persons carrying concealed handguns and other weapons. In direct correspondence with this danger is a need for more sophisticated means of detecting concealed weapons. We have developed a novel concealed-weapons detector based on the principle of low- frequency magnetic imaging. The detector is configured as a portal, and constructs an image of electrically conductive objects transported through it with a potential spatial resolution of approximately 1 inch. Measurements on a breadboard version of the weapons detector have, to date, yielded a resolution of 2 inches. In operation, magnetic dipole radiation, emitted by transmitting antennas in the perimeter of the portal, is scattered from conductive objects and is picked up by receive antennas, also positioned around the portal. With sufficient measurements, each with a different geometry, a solution to the inverse scattering problem can be found. The result is an image of conductive objects in the detector. The detector is sensitive to all metals, semiconductors, and conductive composites. The measured conductivity image formed by the detector is combined with the video signal from a visible CCD camera to form a composite image of persons transiting the detector portal and the conductive objects they are carrying. Accompanying image recognition software could be used to determine the threat level of objects based upon shape, conductivity, and placement on the person of the carrier, and provide cueing, logging, or alarm functions to the operator if suspect weapons are identified. The low- power, low-frequency emissions from the detector are at levels considered safe to humans and medical implants..

  15. Frequency Identification of Vibration Signals Using Video Camera Image Data

    PubMed Central

    Jeng, Yih-Nen; Wu, Chia-Hung

    2012-01-01

    This study showed that an image data acquisition system connecting a high-speed camera or webcam to a notebook or personal computer (PC) can precisely capture most dominant modes of vibration signal, but may involve the non-physical modes induced by the insufficient frame rates. Using a simple model, frequencies of these modes are properly predicted and excluded. Two experimental designs, which involve using an LED light source and a vibration exciter, are proposed to demonstrate the performance. First, the original gray-level resolution of a video camera from, for instance, 0 to 256 levels, was enhanced by summing gray-level data of all pixels in a small region around the point of interest. The image signal was further enhanced by attaching a white paper sheet marked with a black line on the surface of the vibration system in operation to increase the gray-level resolution. Experimental results showed that the Prosilica CV640C CMOS high-speed camera has the critical frequency of inducing the false mode at 60 Hz, whereas that of the webcam is 7.8 Hz. Several factors were proven to have the effect of partially suppressing the non-physical modes, but they cannot eliminate them completely. Two examples, the prominent vibration modes of which are less than the associated critical frequencies, are examined to demonstrate the performances of the proposed systems. In general, the experimental data show that the non-contact type image data acquisition systems are potential tools for collecting the low-frequency vibration signal of a system. PMID:23202026

  16. Frequency identification of vibration signals using video camera image data.

    PubMed

    Jeng, Yih-Nen; Wu, Chia-Hung

    2012-01-01

    This study showed that an image data acquisition system connecting a high-speed camera or webcam to a notebook or personal computer (PC) can precisely capture most dominant modes of vibration signal, but may involve the non-physical modes induced by the insufficient frame rates. Using a simple model, frequencies of these modes are properly predicted and excluded. Two experimental designs, which involve using an LED light source and a vibration exciter, are proposed to demonstrate the performance. First, the original gray-level resolution of a video camera from, for instance, 0 to 256 levels, was enhanced by summing gray-level data of all pixels in a small region around the point of interest. The image signal was further enhanced by attaching a white paper sheet marked with a black line on the surface of the vibration system in operation to increase the gray-level resolution. Experimental results showed that the Prosilica CV640C CMOS high-speed camera has the critical frequency of inducing the false mode at 60 Hz, whereas that of the webcam is 7.8 Hz. Several factors were proven to have the effect of partially suppressing the non-physical modes, but they cannot eliminate them completely. Two examples, the prominent vibration modes of which are less than the associated critical frequencies, are examined to demonstrate the performances of the proposed systems. In general, the experimental data show that the non-contact type image data acquisition systems are potential tools for collecting the low-frequency vibration signal of a system. PMID:23202026

  17. Pulse Inversion Chirp Coded Tissue Harmonic Imaging (PI-CTHI) of Zebrafish Heart Using High Frame Rate Ultrasound Biomicroscopy

    PubMed Central

    Park, Jinhyoung; Huang, Ying; Chen, Ruimin; Lee, Jungwoo; Cummins, Thomas M.; Zhou, Qifa; Lien, Ching-Ling; Shung, K. K.

    2012-01-01

    This paper reports a pulse inversion chirp coded tissue harmonic imaging (PI-CTHI) method for visualizing small animal hearts that provides fine spatial resolution at a high frame rate without sacrificing the echo signal to noise ratio (eSNR). A 40 MHz lithium niobate (LiNbO3) single element transducer is employed to evaluate the performance of PI-CTHI by scanning tungsten wire targets, spherical anechoic voids, and zebrafish hearts. The wire phantom results show that PI-CTHI improves the eSNR by 4 dB from that of conventional pulse inversion tissue harmonic imaging (PI-THI), while still maintaining a spatial resolution of 88 and 110 μm in the axial and lateral directions, respectively. The range side lobe level of PI-CTHI is 11 dB lower than that of band-pass filtered CTHI (or F-CTHI). In the anechoic sphere phantom study, the contrast-to-noise ratio of PI-CTHI is found to be 2.7, indicating a 34% enhancement over conventional PI-THI. Due to such improved eSNR and contrast resolution, blood clots in zebrafish hearts can be readily visualized throughout heart regeneration after 20% of the ventricle is removed. Disappearance of the clots in the early stages of the regeneration has been observed for 7 days without sacrificing the fish. PMID:22930467

  18. Optimum frequency for subsurface-imaging synthetic-aperture radar

    SciTech Connect

    Brock, B.C.; Patitz, W.E.

    1993-05-01

    A subsurface-imaging synthetic-aperture radar (SISAR) has potential for application in areas as diverse as non-proliferation programs for nuclear weapons to environmental monitoring. However, most conventional synthetic-aperture radars operate at higher microwave frequencies which do not significantly penetrate below the soil surface. This study attempts to provide a basis for determining optimum frequencies and frequency ranges which will allow synthetic-aperture imaging of buried targets. Since the radar return from a buried object must compete with the return from surface clutter, the signal-to-clutter ratio is an appropriate measure of performance for a SISAR. A parameter-based modeling approach is used to model the complex dielectric constant of the soil from measured data obtained from the literature. Theoretical random-surface scattering models, based on statistical solutions to Maxwell`s equations, are used to model the clutter. These models are combined to estimate the signal-to-clutter ratio for canonical targets buried in several soil configurations. Initial results indicate that the HF spectrum (3--30 MHz), although it could be used to detect certain targets under some conditions, has limited practical value for use with SISAR, while the upper vhf through uhf spectrum ({approximately}100 MHz--1 GHz) shows the most promise for a general purpose SISAR system. Recommendations are included for additional research.

  19. Coherent Raman spectro-imaging with laser frequency combs.

    PubMed

    Ideguchi, Takuro; Holzner, Simon; Bernhardt, Birgitta; Guelachvili, Guy; Picqué, Nathalie; Hänsch, Theodor W

    2013-10-17

    Advances in optical spectroscopy and microscopy have had a profound impact throughout the physical, chemical and biological sciences. One example is coherent Raman spectroscopy, a versatile technique interrogating vibrational transitions in molecules. It offers high spatial resolution and three-dimensional sectioning capabilities that make it a label-free tool for the non-destructive and chemically selective probing of complex systems. Indeed, single-colour Raman bands have been imaged in biological tissue at video rates by using ultra-short-pulse lasers. However, identifying multiple, and possibly unknown, molecules requires broad spectral bandwidth and high resolution. Moderate spectral spans combined with high-speed acquisition are now within reach using multichannel detection or frequency-swept laser beams. Laser frequency combs are finding increasing use for broadband molecular linear absorption spectroscopy. Here we show, by exploring their potential for nonlinear spectroscopy, that they can be harnessed for coherent anti-Stokes Raman spectroscopy and spectro-imaging. The method uses two combs and can simultaneously measure, on the microsecond timescale, all spectral elements over a wide bandwidth and with high resolution on a single photodetector. Although the overall measurement time in our proof-of-principle experiments is limited by the waiting times between successive spectral acquisitions, this limitation can be overcome with further system development. We therefore expect that our approach of using laser frequency combs will not only enable new applications for nonlinear microscopy but also benefit other nonlinear spectroscopic techniques. PMID:24132293

  20. Optimum frequency for subsurface-imaging synthetic-aperture radar

    SciTech Connect

    Brock, B.C.; Patitz, W.E.

    1993-05-01

    A subsurface-imaging synthetic-aperture radar (SISAR) has potential for application in areas as diverse as non-proliferation programs for nuclear weapons to environmental monitoring. However, most conventional synthetic-aperture radars operate at higher microwave frequencies which do not significantly penetrate below the soil surface. This study attempts to provide a basis for determining optimum frequencies and frequency ranges which will allow synthetic-aperture imaging of buried targets. Since the radar return from a buried object must compete with the return from surface clutter, the signal-to-clutter ratio is an appropriate measure of performance for a SISAR. A parameter-based modeling approach is used to model the complex dielectric constant of the soil from measured data obtained from the literature. Theoretical random-surface scattering models, based on statistical solutions to Maxwell's equations, are used to model the clutter. These models are combined to estimate the signal-to-clutter ratio for canonical targets buried in several soil configurations. Initial results indicate that the HF spectrum (3--30 MHz), although it could be used to detect certain targets under some conditions, has limited practical value for use with SISAR, while the upper vhf through uhf spectrum ([approximately]100 MHz--1 GHz) shows the most promise for a general purpose SISAR system. Recommendations are included for additional research.

  1. Imaging diffusive media using time-independent and time-harmonic sources: dependence of image quality on imaging algorithms, target volume, weight matrix, and view angles

    NASA Astrophysics Data System (ADS)

    Chang, Jenghwa; Aronson, Raphael; Graber, Harry L.; Barbour, Randall L.

    1995-05-01

    We present results examining the dependence of image quality for imaging in dense scattering media as influenced by the choice of parameters pertaining to the physical measurement and factors influencing the efficiency of the computation. The former includes the density of the weight matrix as affected by the target volume, view angle, and source condition. The latter includes the density of the weight matrix and type of algorithm used. These were examined by solving a one-step linear perturbation equation derived from the transport equation using three different algorithms: POCS, CGD, and SART algorithms with contraints. THe above were explored by evaluating four different 3D cylindrical phantom media: a homogeneous medium, an media containing a single black rod on the axis, a single black rod parallel to the axis, and thirteen black rods arrayed in the shape of an 'X'. Solutions to the forward problem were computed using Monte Carlo methods for an impulse source, from which was calculated time- independent and time harmonic detector responses. The influence of target volume on image quality and computational efficiency was studied by computing solution to three types of reconstructions: 1) 3D reconstruction, which considered each voxel individually, 2) 2D reconstruction, which assumed that symmetry along the cylinder axis was know a proiri, 3) 2D limited reconstruction, which assumed that only those voxels in the plane of the detectors contribute information to the detecot readings. The effect of view angle was explored by comparing computed images obtained from a single source, whose position was varied, as well as for the type of tomographic measurement scheme used (i.e., radial scan versus transaxial scan). The former condition was also examined for the dependence of the above on choice of source condition [ i.e., cw (2D reconstructions) versus time-harmonic (2D limited reconstructions) source]. The efficiency of the computational effort was explored

  2. Expansion-maximization-compression algorithm with spherical harmonics for single particle imaging with x-ray lasers

    NASA Astrophysics Data System (ADS)

    Flamant, Julien; Le Bihan, Nicolas; Martin, Andrew V.; Manton, Jonathan H.

    2016-05-01

    In three-dimensional (3D) single particle imaging with x-ray free-electron lasers, particle orientation is not recorded during measurement but is instead recovered as a necessary step in the reconstruction of a 3D image from the diffraction data. Here we use harmonic analysis on the sphere to cleanly separate the angular and radial degrees of freedom of this problem, providing new opportunities to efficiently use data and computational resources. We develop the expansion-maximization-compression algorithm into a shell-by-shell approach and implement an angular bandwidth limit that can be gradually raised during the reconstruction. We study the minimum number of patterns and minimum rotation sampling required for a desired angular and radial resolution. These extensions provide new avenues to improve computational efficiency and speed of convergence, which are critically important considering the very large datasets expected from experiment.

  3. Monitoring the effect of low-level laser therapy in healing process of skin with second harmonic generation imaging techniques

    NASA Astrophysics Data System (ADS)

    Zhang, Xiaoman; Yu, Biying; Weng, Cuncheng; Li, Hui

    2014-11-01

    The 632nm wavelength low intensity He-Ne laser was used to irradiated on 15 mice which had skin wound. The dynamic changes and wound healing processes were observed with nonlinear spectral imaging technology. We observed that:(1)The wound healing process was accelerated by the low-level laser therapy(LLLT);(2)The new tissues produced second harmonic generation (SHG) signals. Collagen content and microstructure differed dramatically at different time pointed along the wound healing. Our observation shows that the low intensity He-Ne laser irradiation can accelerate the healing process of skin wound in mice, and SHG imaging technique can be used to observe wound healing process, which is useful for quantitative characterization of wound status during wound healing process.

  4. Spatial-frequency-based metric for image superresolution.

    PubMed

    Woods, Matthew; Katsaggelos, Aggelos K

    2015-11-01

    The image processing technique known as superresolution (SR) has the potential to allow engineers to specify lower resolution and, therefore, less expensive cameras for a given task by enhancing the base camera's resolution. This is especially true in the remote detection and classification of objects in the environment, such as aircraft or human faces. Performing each of these tasks requires a minimum image "sharpness" which is quantified by a maximum resolvable spatial frequency, which is, in turn, a function of the camera optics, pixel sampling density, and signal-to-noise ratio. Much of the existing SR literature focuses on SR performance metrics for candidate algorithms, such as perceived image quality or peak SNR. These metrics can be misleading because they also credit deblurring and/or denoising in addition to true SR. In this paper, we propose a new, task-based metric where the performance of an SR algorithm is, instead, directly tied to the probability of successfully detecting critical spatial frequencies within the scene. PMID:26560915

  5. Harmonic motion imaging for abdominal tumor detection and high-intensity focused ultrasound ablation monitoring: an in vivo feasibility study in a transgenic mouse model of pancreatic cancer.

    PubMed

    Chen, Hong; Hou, Gary Y; Han, Yang; Payen, Thomas; Palermo, Carmine F; Olive, Kenneth P; Konofagou, Elisa E

    2015-09-01

    Harmonic motion imaging (HMI) is a radiationforce- based elasticity imaging technique that tracks oscillatory tissue displacements induced by sinusoidal ultrasonic radiation force to assess the resulting oscillatory displacement denoting the underlying tissue stiffness. The objective of this study was to evaluate the feasibility of HMI in pancreatic tumor detection and high-intensity focused ultrasound (HIFU) treatment monitoring. The HMI system consisted of a focused ultrasound transducer, which generated sinusoidal radiation force to induce oscillatory tissue motion at 50 Hz, and a diagnostic ultrasound transducer, which detected the axial tissue displacements based on acquired radio-frequency signals using a 1-D cross-correlation algorithm. For pancreatic tumor detection, HMI images were generated for pancreatic tumors in transgenic mice and normal pancreases in wild-type mice. The obtained HMI images showed a high contrast between normal and malignant pancreases with an average peak-to-peak HMI displacement ratio of 3.2. Histological analysis showed that no tissue damage was associated with HMI when it was used for the sole purpose of elasticity imaging. For pancreatic tumor ablation monitoring, the focused ultrasound transducer was operated at a higher acoustic power and longer pulse length than that used in tumor detection to simultaneously induce HIFU thermal ablation and oscillatory tissue displacements, allowing HMI monitoring without interrupting tumor ablation. HMI monitoring of HIFU ablation found significant decreases in the peak-to-peak HMI displacements before and after HIFU ablation with a reduction rate ranging from 15.8% to 57.0%. The formation of thermal lesions after HIFU exposure was confirmed by histological analysis. This study demonstrated the feasibility of HMI in abdominal tumor detection and HIFU ablation monitoring. PMID:26415128

  6. Force correcting atom centred potentials for generalised gradient approximated density functional theory: Approaching hybrid functional accuracy for geometries and harmonic frequencies in small chlorofluorocarbons

    NASA Astrophysics Data System (ADS)

    Anatole von Lilienfeld, O.

    2013-08-01

    Generalised gradient approximated (GGA) density functional theory (DFT) typically overestimates polarisability and bond-lengths, and underestimates force constants of covalent bonds. To overcome this problem we show that one can use empirical force correcting atom centred potentials (FCACPs), parametrised for every nuclear species. Parameters are obtained through minimisation of a penalty functional that explicitly encodes hybrid DFT forces and static polarisabilities of reference molecules. For hydrogen, fluorine, chlorine and carbon the respective reference molecules consist of H2, F2, Cl2 and CH4. The transferability of this approach is assessed for harmonic frequencies in a small set of chlorofluorocarbon molecules. Numerical evidence, gathered for CF4, CCl4, CCl3F, CCl2F2, CClF3, ClF, HF, HCl, CFH3, CF2H2, CF3H, CHCl3, CH2Cl2 and CH3Cl indicates that the GGA+FCACP level of theory yields harmonic frequencies that are significantly more consistent with hybrid DFT values, as well as slightly reduced molecular polarisability.

  7. Molecular structure, Normal Coordinate Analysis, harmonic vibrational frequencies, Natural Bond Orbital, TD-DFT calculations and biological activity analysis of antioxidant drug 7-hydroxycoumarin

    NASA Astrophysics Data System (ADS)

    Sebastian, S.; Sylvestre, S.; Jayarajan, D.; Amalanathan, M.; Oudayakumar, K.; Gnanapoongothai, T.; Jayavarthanan, T.

    2013-01-01

    In this work, we report harmonic vibrational frequencies, molecular structure, NBO and HOMO, LUMO analysis of Umbelliferone also known as 7-hydroxycoumarin (7HC). The optimized geometric bond lengths and bond angles obtained by computation (monomer and dimmer) shows good agreement with experimental XRD data. Harmonic frequencies of 7HC were determined and analyzed by DFT utilizing 6-311+G(d,p) as basis set. The assignments of the vibrational spectra have been carried out with the help of Normal Coordinate Analysis (NCA) following the Scaled Quantum Mechanical Force Field Methodology (SQMFF). The change in electron density (ED) in the σ* and π* antibonding orbitals and stabilization energies E(2) have been calculated by Natural Bond Orbital (NBO) analysis to give clear evidence of stabilization originating in the hyperconjugation of hydrogen-bonded interaction. The energy and oscillator strength calculated by Time-Dependent Density Functional Theory (TD-DFT) complements with the experimental findings. The simulated spectra satisfactorily coincides with the experimental spectra. Microbial activity of studied compounds was tested against Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Escherichia coli, Psuedomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Shigella flexneri, Salmonella typhi and Enterococcus faecalis.

  8. Broadband 120 MHz Impedance Quartz Crystal Microbalance (QCM) with Calibrated Resistance and Quantitative Dissipation for Biosensing Measurements at Higher Harmonic Frequencies

    PubMed Central

    Kasper, Manuel; Traxler, Lukas; Salopek, Jasmina; Grabmayr, Herwig; Ebner, Andreas; Kienberger, Ferry

    2016-01-01

    We developed an impedance quartz crystal microbalance (QCM) approach with the ability to simultaneously record mass changes and calibrated energy dissipation with high sensitivity using an impedance analyzer. This impedance QCM measures frequency shifts and resistance changes of sensing quartz crystals very stable, accurately, and calibrated, thus yielding quantitative information on mass changes and dissipation. Resistance changes below 0.3 Ω were measured with corresponding dissipation values of 0.01 µU (micro dissipation units). The broadband impedance capabilities allow measurements between 20 Hz and 120 MHz including higher harmonic modes of up to 11th order for a 10 MHz fundamental resonance frequency quartz crystal. We demonstrate the adsorbed mass, calibrated resistance, and quantitative dissipation measurements on two biological systems including the high affinity based avidin-biotin interaction and nano-assemblies of polyelectrolyte layers. The binding affinity of a protein-antibody interaction was determined. The impedance QCM is a versatile and simple method for accurate and calibrated resistance and dissipation measurements with broadband measurement capabilities for higher harmonics measurements. PMID:27231946

  9. Detecting vocal fatigue in student singers using acoustic measures of mean fundamental frequency, jitter, shimmer, and harmonics-to-noise ratio

    NASA Astrophysics Data System (ADS)

    Sisakun, Siphan

    2000-12-01

    The purpose of this study is to explore the ability of four acoustic parameters, mean fundamental frequency, jitter, shimmer, and harmonics-to-noise ratio, to detect vocal fatigue in student singers. The participants are 15 voice students, who perform two distinct tasks, data collection task and vocal fatiguing task. The data collection task includes the sustained vowel /a/, reading a standard passage, and self-rate on a vocal fatigue form. The vocal fatiguing task is the vocal practice of musical scores for a total of 45 minutes. The four acoustic parameters are extracted using the software EZVoicePlus. The data analyses are performed to answer eight research questions. The first four questions relate to correlations of the self-rating scale and each of the four parameters. The next four research questions relate to differences in the parameters over time using one-factor repeated measures analysis of variance (ANOVA). The result yields a proposed acoustic profile of vocal fatigue in student singers. This profile is characterized by increased fundamental frequency; slightly decreased jitter; slightly decreased shimmer; and slightly increased harmonics-to-noise ratio. The proposed profile requires further investigation.

  10. Broadband 120 MHz Impedance Quartz Crystal Microbalance (QCM) with Calibrated Resistance and Quantitative Dissipation for Biosensing Measurements at Higher Harmonic Frequencies.

    PubMed

    Kasper, Manuel; Traxler, Lukas; Salopek, Jasmina; Grabmayr, Herwig; Ebner, Andreas; Kienberger, Ferry

    2016-01-01

    We developed an impedance quartz crystal microbalance (QCM) approach with the ability to simultaneously record mass changes and calibrated energy dissipation with high sensitivity using an impedance analyzer. This impedance QCM measures frequency shifts and resistance changes of sensing quartz crystals very stable, accurately, and calibrated, thus yielding quantitative information on mass changes and dissipation. Resistance changes below 0.3 Ω were measured with corresponding dissipation values of 0.01 µU (micro dissipation units). The broadband impedance capabilities allow measurements between 20 Hz and 120 MHz including higher harmonic modes of up to 11th order for a 10 MHz fundamental resonance frequency quartz crystal. We demonstrate the adsorbed mass, calibrated resistance, and quantitative dissipation measurements on two biological systems including the high affinity based avidin-biotin interaction and nano-assemblies of polyelectrolyte layers. The binding affinity of a protein-antibody interaction was determined. The impedance QCM is a versatile and simple method for accurate and calibrated resistance and dissipation measurements with broadband measurement capabilities for higher harmonics measurements. PMID:27231946

  11. Investigation of signal-to-noise ratio in frequency-domain multiphoton fluorescence lifetime imaging microscopy.

    PubMed

    Zhang, Yide; Khan, Aamir A; Vigil, Genevieve D; Howard, Scott S

    2016-07-01

    Multiphoton microscopy (MPM) combined with fluorescence lifetime imaging microscopy (FLIM) has enabled three-dimensional quantitative molecular microscopy in vivo. The signal-to-noise ratio (SNR), and thus the imaging rate of MPM-FLIM, which is fundamentally limited by the shot noise and fluorescence saturation, has not been quantitatively studied yet. In this paper, we investigate the SNR performance of the frequency-domain (FD) MPM-FLIM with two figures of merit: the photon economy in the limit of shot noise, and the normalized SNR in the limit of saturation. The theoretical results and Monte Carlo simulations find that two-photon FD-FLIM requires 50% fewer photons to achieve the same SNR as conventional one-photon FLIM. We also analytically show that the MPM-FD-FLIM can exploit the DC and higher harmonic components generated by nonlinear optical mixing of the excitation light to improve SNR, reducing the required number of photons by an additional 50%. Finally, the effect of fluorophore saturation on the experimental SNR performance is discussed. PMID:27409702

  12. Enhanced speed in fluorescence imaging using beat frequency multiplexing

    NASA Astrophysics Data System (ADS)

    Mikami, Hideharu; Kobayashi, Hirofumi; Wang, Yisen; Hamad, Syed; Ozeki, Yasuyuki; Goda, Keisuke

    2016-03-01

    Fluorescence imaging using radiofrequency-tagged emission (FIRE) is an emerging technique that enables higher imaging speed (namely, temporal resolution) in fluorescence microscopy compared to conventional fluorescence imaging techniques such as confocal microscopy and wide-field microscopy. It works based on the principle that it uses multiple intensity-modulated fields in an interferometric setup as excitation fields and applies frequency-division multiplexing to fluorescence signals. Unfortunately, despite its high potential, FIRE has limited imaging speed due to two practical limitations: signal bandwidth and signal detection efficiency. The signal bandwidth is limited by that of an acousto-optic deflector (AOD) employed in the setup, which is typically 100-200 MHz for the spectral range of fluorescence excitation (400-600 nm). The signal detection efficiency is limited by poor spatial mode-matching between two interfering fields to produce a modulated excitation field. Here we present a method to overcome these limitations and thus to achieve higher imaging speed than the prior version of FIRE. Our method achieves an increase in signal bandwidth by a factor of two and nearly optimal mode matching, which enables the imaging speed limited by the lifetime of the target fluorophore rather than the imaging system itself. The higher bandwidth and better signal detection efficiency work synergistically because higher bandwidth requires higher signal levels to avoid the contribution of shot noise and amplifier noise to the fluorescence signal. Due to its unprecedentedly high-speed performance, our method has a wide variety of applications in cancer detection, drug discovery, and regenerative medicine.

  13. Detecting structural information of scatterers using spatial frequency domain imaging.

    PubMed

    Bodenschatz, Nico; Krauter, Philipp; Nothelfer, Steffen; Foschum, Florian; Bergmann, Florian; Liemert, André; Kienle, Alwin

    2015-11-01

    We demonstrate optical phantom experiments on the phase function parameter γ using spatial frequency domain imaging. The incorporation of two different types of scattering particles allows for control of the optical phantoms’ microscopic scattering properties. By laterally structuring areas with either TiO2 or Al2O3 scattering particles, we were able to obtain almost pure subdiffusive scattering contrast in a single optical phantom. Optical parameter mapping was then achieved using an analytical radiative transfer model revealing the microscopic structural contrast on a macroscopic field of view. As part of our study, we explain several correction and referencing techniques for high spatial frequency analysis and experimentally study the sampling depth of the subdiffusive parameter γ. PMID:26590206

  14. Drive frequency dependent phase imaging in piezoresponse force microscopy

    SciTech Connect

    Bo Huifeng; Kan Yi; Lu Xiaomei; Liu Yunfei; Peng Song; Wang Xiaofei; Cai Wei; Xue Ruoshi; Zhu Jinsong

    2010-08-15

    The drive frequency dependent piezoresponse (PR) phase signal in near-stoichiometric lithium niobate crystals is studied by piezoresponse force microscopy. It is clearly shown that the local and nonlocal electrostatic forces have a great contribution to the PR phase signal. The significant PR phase difference of the antiparallel domains are observed at the contact resonances, which is related to the electrostatic dominated electromechanical interactions of the cantilever and tip-sample system. Moreover, the modulation voltage induced frequency shift at higher eigenmodes could be attributed to the change of indention force depending on the modulation amplitude with a piezoelectric origin. The PR phase of the silicon wafer is also measured for comparison. It is certificated that the electrostatic interactions are universal in voltage modulated scanning probe microscopy and could be extended to other phase imaging techniques.

  15. Second-harmonic generation imaging of semiconductor nanowires with focused vector beams.

    PubMed

    Bautista, Godofredo; Mäkitalo, Jouni; Chen, Ya; Dhaka, Veer; Grasso, Marco; Karvonen, Lasse; Jiang, Hua; Huttunen, Mikko J; Huhtio, Teppo; Lipsanen, Harri; Kauranen, Martti

    2015-03-11

    We use second-harmonic generation (SHG) with focused vector beams to investigate individual vertically aligned GaAs nanowires. Our results provide direct evidence that SHG from oriented nanowires is mainly driven by the longitudinal field along the nanowire growth axis. Consequently, focused radial polarization provides a superior tool to characterize such nanowires compared to linear polarization, also allowing this possibility in the native growth environment. We model our experiments by describing the SHG process for zinc-blende structure and dipolar bulk nonlinearity. PMID:25651302

  16. Monitoring electrical and thermal burns with Spatial Frequency Domain Imaging

    NASA Astrophysics Data System (ADS)

    Ramella-Roman, Jessica

    2011-10-01

    Thermal and electrical injuries are devastating and hard-to-treat clinical lesions. The pathophysiology of these injuries is not fully understood to this day. Further elucidating the natural history of this form of tissue injury could be helpful in offering stage-appropriate therapy. Spatial Frequency Domain Imaging (SFDI) is a novel non-invasive technique that can be used to determine optical properties of biological media. We have developed an experimental apparatus based on SFDI aimed at monitoring parameters of clinical interest such as tissue oxygen saturation, methemoglobin volume fraction, and hemoglobin volume fraction. Co- registered Laser Doppler images of the lesions are also acquired to assess tissue perfusion. Results of experiments conducted on a rat model and discussions on the systemic changes in tissue optical properties before and after injury will be presented.

  17. Method for imaging with low frequency electromagnetic fields

    DOEpatents

    Lee, Ki H.; Xie, Gan Q.

    1994-01-01

    A method for imaging with low frequency electromagnetic fields, and for interpreting the electromagnetic data using ray tomography, in order to determine the earth conductivity with high accuracy and resolution. The imaging method includes the steps of placing one or more transmitters, at various positions in a plurality of transmitter holes, and placing a plurality of receivers in a plurality of receiver holes. The transmitters generate electromagnetic signals which diffuse through a medium, such as earth, toward the receivers. The measured diffusion field data H is then transformed into wavefield data U. The traveltimes corresponding to the wavefield data U, are then obtained, by charting the wavefield data U, using a different regularization parameter .alpha. for each transform. The desired property of the medium, such as conductivity, is then derived from the velocity, which in turn is constructed from the wavefield data U using ray tomography.

  18. Method for imaging with low frequency electromagnetic fields

    DOEpatents

    Lee, K.H.; Xie, G.Q.

    1994-12-13

    A method is described for imaging with low frequency electromagnetic fields, and for interpreting the electromagnetic data using ray tomography, in order to determine the earth conductivity with high accuracy and resolution. The imaging method includes the steps of placing one or more transmitters, at various positions in a plurality of transmitter holes, and placing a plurality of receivers in a plurality of receiver holes. The transmitters generate electromagnetic signals which diffuse through a medium, such as earth, toward the receivers. The measured diffusion field data H is then transformed into wavefield data U. The travel times corresponding to the wavefield data U, are then obtained, by charting the wavefield data U, using a different regularization parameter [alpha] for each transform. The desired property of the medium, such as conductivity, is then derived from the velocity, which in turn is constructed from the wavefield data U using ray tomography. 13 figures.

  19. Theory of high-order harmonic generation and attosecond pulse emission by a low-frequency elliptically polarized laser field

    SciTech Connect

    Strelkov, V. V.

    2006-07-15

    We present a quantum-mechanical theory of xuv generation by an elliptically polarized intense laser field. Our approach is valid when the Keldysh parameter {gamma} is about unity or less, and the driving ellipticity is less than {radical}(2){gamma}. After the photoionization the motion of the electronic wave packet along the major axis of the driving field polarization ellipse is described quasiclassically, whereas the motion in the transverse direction is considered fully quantum mechanically; we also find the condition that allows the reduction of the motion description to a quantum orbit in the polarization plane of the laser field. We use the ionization rate calculated via numerical solution of the three-dimensional Schroedinger equation (TDSE), and take into account the Coulomb modification of the free electronic wave packet. The predictions of our theory for xuv emission agree well with numerical and experimental results. We study the high harmonic intensities and phases as functions of the driving intensity and ellipticity, and also the ellipticity and the rotation angle of the harmonic field polarization ellipse as functions of the driving ellipticity. The atomic response is decomposed into the contributions of different quantum paths. This allows finding a straightforward explanation for the observed dependencies.

  20. Time-frequency analysis of functional optical mammographic images

    NASA Astrophysics Data System (ADS)

    Barbour, Randall L.; Graber, Harry L.; Schmitz, Christoph H.; Tarantini, Frank; Khoury, Georges; Naar, David J.; Panetta, Thomas F.; Lewis, Theophilus; Pei, Yaling

    2003-07-01

    We have introduced working technology that provides for time-series imaging of the hemoglobin signal in large tissue structures. In this study we have explored our ability to detect aberrant time-frequency responses of breast vasculature for subjects with Stage II breast cancer at rest and in response to simple provocations. The hypothesis being explored is that time-series imaging will be sensitive to the known structural and functional malformations of the tumor vasculature. Mammographic studies were conducted using an adjustable hemisheric measuring head containing 21 source and 21 detector locations (441 source-detector pairs). Simultaneous dual-wavelength studies were performed at 760 and 830 nm at a framing rate of ~2.7 Hz. Optical measures were performed on women lying prone with the breast hanging in a pendant position. Two class of measures were performed: (1) 20- minute baseline measure wherein the subject was at rest; (2) provocation studies wherein the subject was asked to perform some simple breathing maneuvers. Collected data were analyzed to identify the time-frequency structure and central tendencies of the detector responses and those of the image time series. Imaging data were generated using the Normalized Difference Method (Pei et al., Appl. Opt. 40, 5755-5769, 2001). Results obtained clearly document three classes of anomalies when compared to the normal contralateral breast. 1) Breast tumors exhibit altered oxygen supply/demand imbalance in response to an oxidative challenge (breath hold). 2) The vasomotor response of the tumor vasculature is mainly depressed and exhibits an altered modulation. 3) The affected area of the breast wherein the altered vasomotor signature is seen extends well beyond the limits of the tumor itself.

  1. Label-free and real-time imaging of dehydration-induced DNA conformational changes in cellular nucleus using second harmonic microscopy

    NASA Astrophysics Data System (ADS)

    Zhuo, Shuangmu; Ni, Ming

    2014-12-01

    Dehydration-induced DNA conformational changes have been probed for the first time with the use of second harmonic microscopy. Unlike conventional approaches, second harmonic microscopy provides a label-free and real-time approach to detect DNA conformational changes. Upon dehydration, cellular DNA undergoes a transition from B- to A-form, whereas cellular nuclei change from invisible to visible under second harmonic microscopy. These results showed that DNA is a second order nonlinear optical material. We further confirmed this by characterizing the nonlinear optical properties of extracted DNA from human cells. Our findings open a new path for SHG imaging. DNA can change its conformations under many circumstances. For example: normal cells turning into cancerous cells and drug molecules binding with DNA. Therefore, the detection of DNA conformational changes with second harmonic microscopy will be a useful tool in cancer therapy and new drug discovery.

  2. Imaging interplanetary CMEs at radio frequency from solar polar orbit

    NASA Astrophysics Data System (ADS)

    Wu, Ji; Sun, Weiying; Zheng, Jianhua; Zhang, Cheng; Liu, Hao; Yan, Jingye; Wang, Chi; Wang, Chuanbing; Wang, Shui

    2011-09-01

    Coronal mass ejections (CMEs) represent a great concentration of mass and energy input into the lower corona. They have come to be recognized as the major driver of physical conditions change in the Sun-Earth system. Consequently, observations of CMEs are important for understanding and ultimately predicting space weather conditions. This paper discusses a proposed mission, the Solar Polar Orbit Radio Telescope (SPORT) mission, which will observe the propagation of interplanetary CMEs to distances of near 0.35 AU from the Sun. The orbit of SPORT is an elliptical solar polar orbit. The inclination angle between the orbit and ecliptic plane should be about 90°. The main payload on board SPORT will be an imaging radiometer working at the meter wavelength band (radio telescope), which can follow the propagation of interplanetary CMEs. The images that are obtained by the radio telescope embody the brightness temperature of the objectives. Due to the very large size required for the antenna aperture of the radio telescope, we adopt interferometric imaging technology to reduce it. Interferometric imaging technology is based on indirect spatial frequency domain measurements plus Fourier transformation. The SPORT spacecraft will also be equipped with a set of optical and in situ measurement instruments such as a EUV solar telescope, a solar wind ion instrument, an energetic particle detector, a magnetometer, a wave detector and a solar radio burst spectrometer.

  3. Image transmission in tactical radio frequency shared network propagation environments

    NASA Astrophysics Data System (ADS)

    White, Kent H.; Wagner, Kerry A.; O'Hanian, Scott

    1997-06-01

    The need to transmit images across tactical radio frequency (rf) links has been identified in army digitization applications. For example, military doctrine requires that tactical functions like identification of battlefield entities as potential targets and battle damage assessment be performed by the soldier. Currently, a key input to these processes is imagery. Therefore, the quality and timeliness of the image directly impact tactical performance. The military is investigating the employment of remote sensors and advanced communications systems to meet this requirement as part of its digitization effort. Army communications systems exist that partially meet this requirement. However, many existing solutions employ these legacy systems in the context of a point-to-point communications architecture. Solutions to the problem of transmitting images across a rf network have not been fully explored. The term network implies that the rf transmission media is common to and shared by multiple subscribers. It is a suite of capabilities that collectively manage media access and information transfer for its subscribers thus providing substantial improvements in effectiveness, efficiency, and robustness. This paper discusses the challenges of transmitting images using one army legacy communications system in a tactical rf network, presents a conceptual framework for attacking the problem, and discusses one solution.

  4. Fast 3D subsurface imaging with stepped-frequency GPR

    NASA Astrophysics Data System (ADS)

    Masarik, Matthew P.; Burns, Joseph; Thelen, Brian T.; Sutter, Lena

    2015-05-01

    This paper investigates an algorithm for forming 3D images of the subsurface using stepped-frequency GPR data. The algorithm is specifically designed for a handheld GPR and therefore accounts for the irregular sampling pattern in the data and the spatially-variant air-ground interface by estimating an effective "ground-plane" and then registering the data to the plane. The algorithm efficiently solves the 4th-order polynomial for the Snell reflection points using a fully vectorized iterative scheme. The forward operator is implemented efficiently using an accelerated nonuniform FFT (Greengard and Lee, 2004); the adjoint operator is implemented efficiently using an interpolation step coupled with an upsampled FFT. The imaging is done as a linearized version of the full inverse problem, which is regularized using a sparsity constraint to reduce sidelobes and therefore improve image localization. Applying an appropriate sparsity constraint, the algorithm is able to eliminate most the surrounding clutter and sidelobes, while still rendering valuable image properties such as shape and size. The algorithm is applied to simulated data, controlled experimental data (made available by Dr. Waymond Scott, Georgia Institute of Technology), and government-provided data with irregular sampling and air-ground interface.

  5. Methods, compositions and kits for imaging cells and tissues using nanoparticles and spatial frequency heterodyne imaging

    DOEpatents

    Rose-Petruck, Christoph; Wands, Jack R.; Rand, Danielle; Derdak, Zoltan; Ortiz, Vivian

    2016-04-19

    Methods, compositions, systems, devices and kits are provided herein for preparing and using a nanoparticle composition and spatial frequency heterodyne imaging for visualizing cells or tissues. In various embodiments, the nanoparticle composition includes at least one of: a nanoparticle, a polymer layer, and a binding agent, such that the polymer layer coats the nanoparticle and is for example a polyethylene glycol, a polyelectrolyte, an anionic polymer, or a cationic polymer, and such that the binding agent that specifically binds the cells or the tissue. Methods, compositions, systems, devices and kits are provided for identifying potential therapeutic agents in a model using the nanoparticle composition and spatial frequency heterodyne imaging.

  6. Harmonic engine

    DOEpatents

    Bennett, Charles L.; Sewall, Noel; Boroa, Carl

    2014-08-19

    An engine based on a reciprocating piston engine that extracts work from pressurized working fluid. The engine includes a harmonic oscillator inlet valve capable of oscillating at a resonant frequency for controlling the flow of working fluid into of the engine. In particular, the inlet valve includes an inlet valve head and a spring arranged together as a harmonic oscillator so that the inlet valve head is moveable from an unbiased equilibrium position to a biased closed position occluding an inlet. Upon releasing the inlet valve the inlet valve head undergoes a single oscillation past the equilibrium positio to a maximum open position and returns to a biased return position close to the closed position to choke the flow and produce a pressure drop across the inlet valve causing the inlet valve to close. Protrusions carried either by the inlet valve head or piston head are used to bump open the inlet valve from the closed position and initiate the single oscillation of the inlet valve head, and protrusions carried either by the outlet valve head or piston head are used to close the outlet valve ahead of the bump opening of the inlet valve.

  7. Reconstruction of sectional images in frequency-domain based photoacoustic imaging.

    PubMed

    Zhu, Banghe; Sevick-Muraca, Eva M

    2011-11-01

    Photoacoustic (PA) imaging is based upon the generation of an ultrasound pulse arising from subsurface tissue absorption due to pulsed laser excitation, and measurement of its surface time-of-arrival. Expensive and bulky pulsed lasers with high peak fluence powers may provide shortcomings for applications of PA imaging in medicine and biology. These limitations may be overcome with the frequency-domain PA measurements, which employ modulated rather than pulsed light to generate the acoustic wave. In this contribution, we model the single modulation frequency based PA pressures on the measurement plane through the diffraction approximation and then employ a convolution approach to reconstruct the sectional image slices. The results demonstrate that the proposed method with appropriate data post-processing is capable of recovering sectional images while suppressing the defocused noise resulting from the other sections. PMID:22109207

  8. In vivo feasibility of real-time monitoring of focused ultrasound surgery (FUS) using harmonic motion imaging (HMI).

    PubMed

    Maleke, Caroline; Konofagou, Elisa E

    2010-01-01

    In this study, the Harmonic Motion Imaging for Focused Ultrasound (HMIFU) technique is applied to monitor changes in mechanical properties of tissues during thermal therapy in a transgenic breast cancer mouse model in vivo. An HMIFU system, composed of a 4.5-MHz focused ultrasound (FUS) and a 3.3-MHz phased-array imaging transducer, was mechanically moved to image and ablate the entire tumor. The FUS transducer was driven by an amplitude-modulated (AM) signal at 15 Hz. The acoustic intensity ( I(spta)) was equal to 1050 W/cm(2) at the focus. A digital low-pass filter was used to filter out the spectrum of the FUS beam and its harmonics prior to displacement estimation. The resulting axial displacement was estimated using 1-D cross-correlation on the acquired RF signals. Results from two mice with eight lesions formed in each mouse (16 lesions total) showed that the average peak-to-peak displacement amplitude before and after lesion formation was respectively equal to 17.34 +/- 1.34 microm and 10.98 +/- 1.82 microm ( p < 0.001). Cell death was also confirmed by hematoxylin and eosin histology. HMI displacement can be used to monitor the relative tissue stiffness changes in real time during heating so that the treatment procedure can be performed in a time-efficient manner. The HMIFU system may, therefore, constitute a cost-efficient and reliable alternative for real-time monitoring of thermal ablation. PMID:19643703

  9. Simulation study of amplitude-modulated (AM) harmonic motion imaging (HMI) for stiffness contrast quantification with experimental validation.

    PubMed

    Maleke, Caroline; Luo, Jianwen; Gamarnik, Viktor; Lu, Xin L; Konofagou, Elisa E

    2010-07-01

    The objective of this study is to show that Harmonic Motion Imaging (HMI) can be used as a reliable tumor-mapping technique based on the tumor's distinct stiffness at the early onset of disease. HMI is a radiation-force-based imaging method that generates a localized vibration deep inside the tissue to estimate the relative tissue stiffness based on the resulting displacement amplitude. In this paper, a finite-element model (FEM) study is presented, followed by an experimental validation in tissue-mimicking polyacrylamide gels and excised human breast tumors ex vivo. This study compares the resulting tissue motion in simulations and experiments at four different gel stiffnesses and three distinct spherical inclusion diameters. The elastic moduli of the gels were separately measured using mechanical testing. Identical transducer parameters were used in both the FEM and experimental studies, i.e., a 4.5-MHz single-element focused ultrasound (FUS) and a 7.5-MHz diagnostic (pulse-echo) transducer. In the simulation, an acoustic pressure field was used as the input stimulus to generate a localized vibration inside the target. Radiofrequency (rf) signals were then simulated using a 2D convolution model. A one-dimensional cross-correlation technique was performed on the simulated and experimental rf signals to estimate the axial displacement resulting from the harmonic radiation force. In order to measure the reliability of the displacement profiles in estimating the tissue stiffness distribution, the contrast-transfer efficiency (CTE) was calculated. For tumor mapping ex vivo, a harmonic radiation force was applied using a 2D raster-scan technique. The 2D HMI images of the breast tumor ex vivo could detect a malignant tumor (20 x 10 mm2) surrounded by glandular and fat tissues. The FEM and experimental results from both gels and breast tumors ex vivo demonstrated that HMI was capable of detecting and mapping the tumor or stiff inclusion with various diameters or

  10. High frequency volume coils for clinical NMR imaging and spectroscopy.

    PubMed

    Vaughan, J T; Hetherington, H P; Otu, J O; Pan, J W; Pohost, G M

    1994-08-01

    A tuned transmission line resonator has been developed in theory and in practical design for the clinical NMR volume coil application at 4.1 tesla. The distributed circuit transmission line resonator was designed for high frequency, large conductive volume applications where conventional lumped element coil designs perform less efficiently. The resonator design has made use of a resonant coaxial cavity, which could be variably tuned to the Larmor frequency of interest by tunable transmission line elements. Large head- and body-sized volumes, high efficiencies, and broad tuning ranges have been shown to be characteristic of the transmission line resonator to frequencies of 500 MHz. The B1 homogeneity of the resonator has been demonstrated to be a function of the electromagnetic properties of the load itself. By numerically solving Maxwell's equations for the fully time-dependent B1 field, coil homogeneity was predicted with finite-element models of anatomic structure, and inhomogeneities corrected for. A how-to exposition of coil design and construction has been included. Simple methods of quadrature driving and double tuning the transmission line resonator have also been presented. Human head images obtained with a tuned transmission line resonator at 175 MHz have clearly demonstrated uncompromised high field advantages of signal-to-noise and spatial resolution. PMID:7968443

  11. High-throughput screening of drug-lipid membrane interactions via counter-propagating second harmonic generation imaging.

    PubMed

    Nguyen, Trang T; Conboy, John C

    2011-08-01

    Here we report the use of counter-propagating second harmonic generation (SHG) to image the interactions between the local anesthetic tetracaine and a multicomponent planar supported lipid bilayer array in a label-free manner. The lipid bilayer arrays, prepared using a 3D continuous flow microspotter, allow the effects of lipid phase and cholesterol content on tetracaine binding to be examined simultaneously. SHG images show that tetracaine has a higher binding affinity to liquid-crystalline phase lipids than to solid-gel phase lipids. The presence of 28 mol % cholesterol decreased the binding affinity of tetracaine to bilayers composed of the mixed chain lipid, 1-steroyl-2-oleoyl-sn-glycero-3-phophocholine (SOPC), and the saturated lipids 1,2-dimyristoyl-sn-glycero-3-phophocholine (DMPC) and 1,2-dipamitoyl-sn-glycero-3-phophocholine (DPPC) while having no effect on diunsaturated 1,2-dioleoyl-sn-glycero-3-phophocholine (DOPC). The maximum surface excess of tetracaine increases with the degree of unsaturation of the phospholipids and decreases with cholesterol in the lipid bilayers. The paper demonstrates that SHG imaging is a sensitive technique that can directly image and quantitatively measure the association of a drug to a multicomponent lipid bilayer array, providing a high-throughput means to assess drug-membrane interactions. PMID:21696170

  12. Multi-view second-harmonic generation imaging of mouse tail tendon via reflective micro-prisms.

    PubMed

    Wen, Bruce; Campbell, Kirby R; Cox, Benjamin L; Eliceiri, Kevin W; Superfine, Richard; Campagnola, Paul J

    2015-07-01

    Here we experimentally show that second-harmonic generation (SHG) imaging is not sensitive to collagen fibers oriented parallel to the direction of laser propagation and, as a consequence, can potentially miss important structural information. As an alternative approach, we demonstrate the use of reflective micro-prisms to enable multi-view SHG imaging of mouse tail tendon by redirecting the focused excitation and collection of subsequent emission. Our approach data corroborates the theoretical treatment on vanishing and nonvanishing orientations, where fibers along the laser direction are largely transparent by SHG. In strong contrast, the two-photon excited fluorescence of dye-labeled collagen fibers is isotropic and is not subject to this constraint. We utilized Pearson correlation to quantify differences in fluorescent and backward detected SHG images of the tendon fiber structure, where the SHG and TPEF were highly statistically correlated (0.6-0.8) for perpendicular excitation but were uncorrelated for excitation parallel to the fiber axis. The results suggest that improved imaging of 3D collagen structure is possible with multi-view SHG microscopy. PMID:26125402

  13. Coupled third-order simplified spherical harmonics and diffusion equation-based fluorescence tomographic imaging of liver cancer

    NASA Astrophysics Data System (ADS)

    Chen, Xueli; Sun, Fangfang; Yang, Defu; Liang, Jimin

    2015-09-01

    For fluorescence tomographic imaging of small animals, the liver is usually regarded as a low-scattering tissue and is surrounded by adipose, kidneys, and heart, all of which have a high scattering property. This leads to a breakdown of the diffusion equation (DE)-based reconstruction method as well as a heavy computational burden for the simplified spherical harmonics equation (SPN). Coupling the SPN and DE provides a perfect balance between the imaging accuracy and computational burden. The coupled third-order SPN and DE (CSDE)-based reconstruction method is developed for fluorescence tomographic imaging. This is achieved by doubly using the CSDE for the excitation and emission processes of the fluorescence propagation. At the same time, the finite-element method and hybrid multilevel regularization strategy are incorporated in inverse reconstruction. The CSDE-based reconstruction method is first demonstrated with a digital mouse-based liver cancer simulation, which reveals superior performance compared with the SPN and DE-based methods. It is more accurate than the DE-based method and has lesser computational burden than the SPN-based method. The feasibility of the proposed approach in applications of in vivo studies is also illustrated with a liver cancer mouse-based in situ experiment, revealing its potential application in whole-body imaging of small animals.

  14. Multi-view second-harmonic generation imaging of mouse tail tendon via reflective micro-prisms

    PubMed Central

    Wen, Bruce; Campbell, Kirby R.; Cox, Benjamin L.; Eliceiri, Kevin W.; Superfine, Richard; Campagnola, Paul J.

    2016-01-01

    Here we experimentally show that second-harmonic generation (SHG) imaging is not sensitive to collagen fibers oriented parallel to the direction of laser propagation and, as a consequence, can potentially miss important structural information. As an alternative approach, we demonstrate the use of reflective micro-prisms to enable multi-view SHG imaging of mouse tail tendon by redirecting the focused excitation and collection of subsequent emission. Our approach data corroborates the theoretical treatment on vanishing and nonvanishing orientations, where fibers along the laser direction are largely transparent by SHG. In strong contrast, the two-photon excited fluorescence of dye-labeled collagen fibers is isotropic and is not subject to this constraint. We utilized Pearson correlation to quantify differences in fluorescent and backward detected SHG images of the tendon fiber structure, where the SHG and TPEF were highly statistically correlated (0.6–0.8) for perpendicular excitation but were uncorrelated for excitation parallel to the fiber axis. The results suggest that improved imaging of 3D collagen structure is possible with multi-view SHG microscopy. PMID:26125402

  15. High-Throughput Screening of Drug-Lipid Membrane Interactions via Counter-Propagating Second Harmonic Generation Imaging

    PubMed Central

    Nguyen, Trang T.; Conboy, John C.

    2011-01-01

    Here we report the use of counter-propagating second harmonic generation (SHG) to image the interactions between the local anesthetic tetracaine and a multi-component planar supported lipid bilayer array in a label-free manner. The lipid bilayer arrays, prepared using a 3D continuous flow microspotter, allow the effects of lipid phase and cholesterol content on tetracaine binding to be examined simultaneously. SHG images show that tetracaine has a higher binding affinity to liquid-crystalline phase lipids than to solid-gel phase lipids. The presence of 28 mol % cholesterol decreased the binding affinity of tetracaine to bilayers composed of the mixed chain lipid, 1-steroyl-2-oleoyl-sn-glycero-3-phophocholine (SOPC) and the saturated lipids 1,2-dimyristoyl-sn-glycero-3-phophocholine (DMPC) and 1,2-dipamitoyl-sn-glycero-3-phophocholine (DPPC) while having no effect on di-unsaturated 1,2-dioleoyl-sn-glycero-3-phophocholine (DOPC). The maximum surface excess of tetracaine increases with the degree of unsaturation of the phospholipids and decreases with cholesterol in the lipid bilayers. The paper demonstrates that SHG imaging is a sensitive technique that can directly image and quantitatively measure the association of a drug to a multi-component lipid bilayer array, providing a high-throughput means to assess drug-membrane interactions. PMID:21696170

  16. Coupled third-order simplified spherical harmonics and diffusion equation-based fluorescence tomographic imaging of liver cancer.

    PubMed

    Chen, Xueli; Sun, Fangfang; Yang, Defu; Liang, Jimin

    2015-01-01

    For fluorescence tomographic imaging of small animals, the liver is usually regarded as a low-scattering tissue and is surrounded by adipose, kidneys, and heart, all of which have a high scattering property. This leads to a breakdown of the diffusion equation (DE)–based reconstruction method as well as a heavy computational burden for the simplified spherical harmonics equation (SP(N)). Coupling the SP(N) and DE provides a perfect balance between the imaging accuracy and computational burden. The coupled third-order SPN and DE (CSDE)-based reconstruction method is developed for fluorescence tomographic imaging. This is achieved by doubly using the CSDE for the excitation and emission processes of the fluorescence propagation. At the same time, the finite-element method and hybrid multilevel regularization strategy are incorporated in inverse reconstruction. The CSDE-based reconstruction method is first demonstrated with a digital mouse-based liver cancer simulation, which reveals superior performance compared with the SPN and DE-based methods. It is more accurate than the DE-based method and has lesser computational burden than the SPN-based method. The feasibility of the proposed approach in applications of in vivo studies is also illustrated with a liver cancer mouse-based in situ experiment, revealing its potential application in whole-body imaging of small animals. PMID:26385654

  17. 2D harmonic filtering of MR phase images in multicenter clinical setting: toward a magnetic signature of cerebral microbleeds.

    PubMed

    Kaaouana, Takoua; de Rochefort, Ludovic; Samaille, Thomas; Thiery, Nathalie; Dufouil, Carole; Delmaire, Christine; Dormont, Didier; Chupin, Marie

    2015-01-01

    Cerebral microbleeds (CMBs) have emerged as a new imaging marker of small vessel disease. Composed of hemosiderin, CMBs are paramagnetic and can be detected with MRI sequences sensitive to magnetic susceptibility (typically, gradient recalled echo T2* weighted images). Nevertheless, their identification remains challenging on T2* magnitude images because of confounding structures and lesions. In this context, T2* phase image may play a key role in better characterizing CMBs because of its direct relationship with local magnetic field variations due to magnetic susceptibility difference. To address this issue, susceptibility-based imaging techniques were proposed, such as Susceptibility Weighted Imaging (SWI) and Quantitative Susceptibility Mapping (QSM). But these techniques have not yet been validated for 2D clinical data in multicenter settings. Here, we introduce 2DHF, a fast 2D phase processing technique embedding both unwrapping and harmonic filtering designed for data acquired in 2D, even with slice-to-slice inconsistencies. This method results in internal field maps which reveal local field details due to magnetic inhomogeneity within the region of interest only. This technique is based on the physical properties of the induced magnetic field and should yield consistent results. A synthetic phantom was created for numerical simulations. It simulates paramagnetic and diamagnetic lesions within a 'brain-like' tissue, within a background. The method was evaluated on both this synthetic phantom and multicenter 2D datasets acquired in standardized clinical setting, and compared with two state-of-the-art methods. It proved to yield consistent results on synthetic images and to be applicable and robust on patient data. As a proof-of-concept, we finally illustrate that it is possible to find a magnetic signature of CMBs and CMCs on internal field maps generated with 2DHF on 2D clinical datasets that give consistent results with CT-scans in a subsample of 10 subjects

  18. Benchmark Structures and Harmonic Vibrational Frequencies Near the CCSD(T) Complete Basis Set Limit for Small Water Clusters: (H2O)n = 2, 3, 4, 5, 6.

    PubMed

    Howard, J Coleman; Tschumper, Gregory S

    2015-05-12

    A series of (H2O)n clusters ranging from the dimer to the hexamer have been characterized with the CCSD(T) and the 2-body:Many-body CCSD(T):MP2 methods near the complete basis set (CBS) limit to generate benchmark-quality optimized structures and harmonic vibrational frequencies for these important systems. Quadruple-ζ correlation-consistent basis sets that augment the O atoms with diffuse functions have been employed in the analytic computation of harmonic vibrational frequencies for the global minima of the dimer, trimer, tetramer, and pentamer as well as the ring, book, cage, and prism isomers of the hexamer. Prior calibration [J. Chem. Phys. 2013, 139, 184113 and J. Chem. Theory Comput. 2014, 10, 5426] suggests that harmonic frequencies computed with this approach will lie within a few cm(-1) of the canonical CCSD(T) CBS limit. These data are used as reference values to gauge the performance of harmonic frequencies obtained with other ab initio methods (e.g., LCCSD(T) and MP2) and water potentials (e.g., TTM3-F and WHBB). This comparison reveals that it is far more challenging to converge harmonic vibrational frequencies for the bound OH stretching modes in these (H2O)n clusters to the CCSD(T) CBS limit than the free OH stretches, the n intramonomer HOH bending modes and even the 6n - 6 intermonomer modes. Deviations associated with the bound OH stretching harmonic frequencies increase rapidly with the size of the cluster for all methods and potentials examined, as do the corresponding frequency shifts relative to the monomer OH stretches. PMID:26574415

  19. Interferometric SAR imaging by transmitting stepped frequency chaotic noise signals

    NASA Astrophysics Data System (ADS)

    Zhang, Yunhua; Gu, Xiang; Zhai, Wenshuai; Dong, Xiao; Shi, Xiaojin; Kang, Xueyan

    2015-10-01

    Noise radar has been applied in many fields since it was proposed more than 50 years ago. However, it has not been applied to interferometric SAR imaging yet as far as we know. This paper introduces our recent work on interferometric noise radar. An interferometric SAR system was developed which can transmit both chirp signal and chaotic noise signal (CNS) at multiple carrier frequencies. An airborne experiment with this system by transmitting both signals was carried out, and the data were processed to show the capability of interferometric SAR imaging with CNS. The results shows that although the interferometric phase quality of CNS is degraded due to the signal to noise ratio (SNR) is lower compared with that of chirp signal, we still can get satisfied DEM after multi-looking processing. Another work of this paper is to apply compressed sensing (CS) theory to the interferometric SAR imaging with CNS. The CS theory states that if a signal is sparse, then it can be accurately reconstructed with much less sampled data than that regularly required according to Nyquist Sampling Theory. To form a structured random matrix, if the transmitted signal is of fixed waveform, then random subsampling is needed. However, if the transmitted signal is of random waveform, then only uniform subsampling is needed. This is another advantage of noise signal. Both the interferometric phase images and the DEMs by regular method and by CS method are processed with results compared. It is shown that the degradation of interferometric phases due to subsampling is larger than that of amplitude image.

  20. Demonstration of An Image Rejection Mixer for High Frequency Applications (26-36 GHz)

    NASA Technical Reports Server (NTRS)

    Bankston, Cheryl D.; Carlstrom, John E.

    1999-01-01

    A new high frequency image-rejection mixer was successfully tested in a 26-36 GHz band receiver. This paper briefly describes the motivation for implementation of an image rejection mixer in a receiver system, the basic operation of an image rejection mixer, and the development and testing of an image rejection mixer for a high frequency, cryogenic receiver system.

  1. Second harmonic generation (SHG) and two-photon fluorescence (TPF) contrast imaging in biomaterial analysis

    NASA Astrophysics Data System (ADS)

    Lang, Xuye; Lyubovitsky, Julia

    2015-07-01

    Collagen hydrogels are natural biomaterials that comprise 3D networks of high water content and have viscoelastic properties and biocompatibility similar to native tissues. Consequently, these materials play an important role in tissue engineering and regenerative medicine for quite some time. Second harmonic generation (SHG) and two-photon fluorescence (TPF) contrasts transpire as valuable label-free spectroscopic probes for analysis of these biomaterials and this presentation will report the structural, mechanical and physicochemical parameters leading to the observed optical SHG and TPF effects in synthesized 3D collagen hydrogels. We will present results regarding understanding the dependency of collagen fiber formation on ion types, new results regarding strengthening of these biomaterials with a nontoxic chemical cross-linker genipin and polarization selection of collagen fibers' orientations.

  2. Polarization-resolved second-harmonic generation imaging for liver fibrosis assessment without labeling

    NASA Astrophysics Data System (ADS)

    Lin, Jian; Pan, Shiying; Zheng, Wei; Huang, Zhiwei

    2013-10-01

    We apply the polarization-resolved second-harmonic generation (PR-SHG) microscopy to investigate the changes of collagen typings (type I vs type III) and collagen fibril orientations of liver tissue in bile-duct-ligation (BDL) rat models. The PR-SHG results show that the second-order susceptibility tensor ratios (χ31/χ15 and χ33/χ15) of collagen fibers increase with liver fibrotic progression after BDL surgery, reflecting an increase of the type III collagen component with the severity of liver fibrosis; and the square root of the collagen type III to type I ratio linearly correlates (R2 = 0.98) with histopathological scores. Furthermore, the collagen fibril orientations become more random with liver fibrosis transformation as compared to normal liver tissue. This work demonstrates that PR-SHG microscopy has the potential for label-free diagnosis and characterization of liver fibrosis based on quantitative analysis of collagen typings and fibril orientations.

  3. Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals.

    PubMed

    Newman, Justin A; Scarborough, Nicole M; Pogranichniy, Nicholas R; Shrestha, Rashmi K; Closser, Richard G; Das, Chittaranjan; Simpson, Garth J

    2015-07-01

    The second-harmonic generation (SHG) activity of protein crystals was found to be enhanced by up to ∼1000-fold by the intercalation of SHG phores within the crystal lattice. Unlike the intercalation of fluorophores, the SHG phores produced no significant background SHG from solvated dye or from dye intercalated into amorphous aggregates. The polarization-dependent SHG is consistent with the chromophores adopting the symmetry of the crystal lattice. In addition, the degree of enhancement for different symmetries of dyes is consistent with theoretical predictions based on the molecular nonlinear optical response. Kinetics studies indicate that intercalation arises over a timeframe of several minutes in lysozyme, with detectable enhancements within seconds. These results provide a potential means to increase the overall diversity of protein crystals and crystal sizes amenable to characterization by SHG microscopy. PMID:26143918

  4. Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals

    PubMed Central

    Newman, Justin A.; Scarborough, Nicole M.; Pogranichniy, Nicholas R.; Shrestha, Rashmi K.; Closser, Richard G.; Das, Chittaranjan; Simpson, Garth J.

    2015-01-01

    The second-harmonic generation (SHG) activity of protein crystals was found to be enhanced by up to ∼1000-fold by the intercalation of SHG phores within the crystal lattice. Unlike the intercalation of fluorophores, the SHG phores produced no significant background SHG from solvated dye or from dye intercalated into amorphous aggregates. The polarization-dependent SHG is consistent with the chromophores adopting the symmetry of the crystal lattice. In addition, the degree of enhancement for different symmetries of dyes is consistent with theoretical predictions based on the molecular nonlinear optical response. Kinetics studies indicate that intercalation arises over a timeframe of several minutes in lysozyme, with detectable enhancements within seconds. These results provide a potential means to increase the overall diversity of protein crystals and crystal sizes amenable to characterization by SHG microscopy. PMID:26143918

  5. Microscopic imaging of glyceraldehyde-induced tissue glycation with intrinsic second harmonic generation and two-photon fluorescence contrasts

    NASA Astrophysics Data System (ADS)

    Hwang, Yu Jer; Granelli, Joseph; Tirumalasetty, Manasa; Lyubovitsky, Julia

    2013-02-01

    The bioinspired approaches to tissue strengthening and preservation rely on non-toxic cross-linking agents one of which is glyceraldehyde. In this study we used multiphoton microscopy that employs second harmonic generation (SHG) contrast to evaluate collagen microstructures and two-photon fluorescence (TPF) contrast to monitor progression of cross-linking upon treatment of tissues with glyceraldehyde. We examined collagen hydrogels assembled at 37 °C and 27 °C, bovine scleral and corneal tissues, skin as well as rat tail tendons. The results show a different effect of glyceraldehyde on collagen microstructures within the above tissues. This effect depends on the original microstructural assembly of collagen within a specific tissue. Our data suggests that epidermis (in skin and cornea) will protect collagen from cross-linking with glyceraldehyde. The work highlights benefits of monitoring progression of collagen cross-linking and effects of cross-linking on fiber microstructures as imaged with SHG and TPF signals.

  6. Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage.

    PubMed

    Houle, Marie-Andrée; Couture, Charles-André; Bancelin, Stéphane; Van der Kolk, Jarno; Auger, Etienne; Brown, Cameron; Popov, Konstantin; Ramunno, Lora; Légaré, François

    2015-11-01

    Collagen ultrastructure plays a central role in the function of a wide range of connective tissues. Studying collagen structure at the microscopic scale is therefore of considerable interest to understand the mechanisms of tissue pathologies. Here, we use second harmonic generation microscopy to characterize collagen structure within bone and articular cartilage in human knees. We analyze the intensity dependence on polarization and discuss the differences between Forward and Backward images in both tissues. Focusing on articular cartilage, we observe an increase in Forward/Backward ratio from the cartilage surface to the bone. Coupling these results to numerical simulations reveals the evolution of collagen fibril diameter and spatial organization as a function of depth within cartilage. PMID:26349534

  7. Feasibility of High Frequency Acoustic Imaging for Inspection of Containments

    SciTech Connect

    C.N. Corrado; J.E. Bondaryk; V. Godino

    1998-08-01

    The Nuclear Regulatory Commission has a program at the Oak Ridge National Laboratory to provide assistance in their assessment of the effects of potential degradation on the structural integrity and Ieaktightness of metal containment vessels and steel liners of concrete containment in nuclear power plants. One of the program objectives is to identify a technique(s) for inspection of inaccessible portions of the containment pressure boundary. Acoustic imaging has been identified as one of these potential techniques. A numerical feasibility study investigated the use of high-frequency bistatic acoustic imaging techniques for inspection of inaccessible portions of the metallic pressure boundary of nuclear power plant containment. The range-dependent version of the OASES Code developed at the Massachusetts Institute of Technology was utilized to perform a series of numerical simulations. OASES is a well developed and extensively tested code for evaluation of the acoustic field in a system of stratified fluid and/or elastic layers. Using the code, an arbitrary number of fluid or solid elastic layers are interleaved, with the outer layers modeled as halfspaces. High frequency vibrational sources were modeled to simulate elastic waves in the steel. The received field due to an arbitrary source array can be calculated at arbitrary depth and range positions. In this numerical study, waves that reflect and scatter from surface roughness caused by modeled degradations (e.g., corrosion) are detected and used to identify and map the steel degradation. Variables in the numerical study included frequency, flaw size, interrogation distance, and sensor incident angle.Based on these analytical simulations, it is considered unlikely that acoustic imaging technology can be used to investigate embedded steel liners of reinforced concrete containment. The thin steel liner and high signal losses to the concrete make this application difficult. Results for portions of steel containment

  8. Direct imaging of radio-frequency modes via traveling wave magnetic resonance imaging

    NASA Astrophysics Data System (ADS)

    Tonyushkin, A.; Deelchand, D. K.; Van de Moortele, P.-F.; Adriany, G.; Kiruluta, A.

    2016-01-01

    We demonstrate an experimental method for direct 2D and 3D imaging of magnetic radio-frequency (rf) field distribution in metal-dielectric structures based on traveling wave (TW) magnetic resonance imaging (MRI) at ultra-high field (>7 T). The typical apparatus would include an ultra-high field whole body or small bore MRI scanner, waveguide elements filled with MRI active dielectrics with predefined electric and magnetic properties, and TW rf transmit-receive probes. We validated the technique by obtaining TW MR images of the magnetic field distribution of the rf modes of circular waveguide filled with deionized water in a 16.4 T small-bore MRI scanner and compared the MR images with numerical simulations. Our MRI technique opens up a practical non-perturbed way of imaging of previously inaccessible rf field distribution of modes inside various shapes metal waveguides with inserted dielectric objects, including waveguide mode converters and transformers.

  9. Dual-frequency acoustic droplet vaporization detection for medical imaging.

    PubMed

    Arena, Christopher B; Novell, Anthony; Sheeran, Paul S; Puett, Connor; Moyer, Linsey C; Dayton, Paul A

    2015-09-01

    Liquid-filled perfluorocarbon droplets emit a unique acoustic signature when vaporized into gas-filled microbubbles using ultrasound. Here, we conducted a pilot study in a tissue-mimicking flow phantom to explore the spatial aspects of droplet vaporization and investigate the effects of applied pressure and droplet concentration on image contrast and axial and lateral resolution. Control microbubble contrast agents were used for comparison. A confocal dual-frequency transducer was used to transmit at 8 MHz and passively receive at 1 MHz. Droplet signals were of significantly higher energy than microbubble signals. This resulted in improved signal separation and high contrast-to-tissue ratios (CTR). Specifically, with a peak negative pressure (PNP) of 450 kPa applied at the focus, the CTR of B-mode images was 18.3 dB for droplets and -0.4 for microbubbles. The lateral resolution was dictated by the size of the droplet activation area, with lower pressures resulting in smaller activation areas and improved lateral resolution (0.67 mm at 450 kPa). The axial resolution in droplet images was dictated by the size of the initial droplet and was independent of the properties of the transmit pulse (3.86 mm at 450 kPa). In post-processing, time-domain averaging (TDA) improved droplet and microbubble signal separation at high pressures (640 kPa and 700 kPa). Taken together, these results indicate that it is possible to generate high-sensitivity, high-contrast images of vaporization events. In the future, this has the potential to be applied in combination with droplet-mediated therapy to track treatment outcomes or as a standalone diagnostic system to monitor the physical properties of the surrounding environment. PMID:26415125

  10. Fast ion energy distribution from third harmonic radio frequency heating measured with a single crystal diamond detector at the Joint European Torus.

    PubMed

    Nocente, M; Cazzaniga, C; Tardocchi, M; Binda, F; Eriksson, J; Giacomelli, L; Muraro, A; Rebai, M; Sharapov, S; Gorini, G

    2015-10-01

    Neutron spectroscopy measurements with a single crystal diamond detector have been carried out at JET, for the first time in an experiment aimed at accelerating deuterons to MeV energies with radio frequency heating at the third harmonic. Data are interpreted by means of the expected response function of the detector and are used to extract parameters of the highly non-Maxwellian distribution function generated in this scenario. A comparison with observations using a time of flight and liquid scintillator neutron spectrometers is also presented. The results demonstrate the capability of diamond detectors to contribute to fast ion physics studies at JET and are of more general relevance in view of the application of such detectors for spectroscopy measurements in the neutron camera of next step tokamak devices. PMID:26520949

  11. Fast ion energy distribution from third harmonic radio frequency heating measured with a single crystal diamond detector at the Joint European Torus

    SciTech Connect

    Nocente, M.; Rebai, M.; Gorini, G.; Cazzaniga, C.; Tardocchi, M.; Giacomelli, L.; Muraro, A.; Binda, F.; Eriksson, J.; Sharapov, S.; Collaboration:

    2015-10-15

    Neutron spectroscopy measurements with a single crystal diamond detector have been carried out at JET, for the first time in an experiment aimed at accelerating deuterons to MeV energies with radio frequency heating at the third harmonic. Data are interpreted by means of the expected response function of the detector and are used to extract parameters of the highly non-Maxwellian distribution function generated in this scenario. A comparison with observations using a time of flight and liquid scintillator neutron spectrometers is also presented. The results demonstrate the capability of diamond detectors to contribute to fast ion physics studies at JET and are of more general relevance in view of the application of such detectors for spectroscopy measurements in the neutron camera of next step tokamak devices.

  12. Optical second-harmonic imaging of Pb{sub x}Cd{sub 1-x}Te ternary alloys

    SciTech Connect

    Scheidt, T.; Rohwer, E.G.; Bergmann, H.M. von; Saucedo, E.; Dieguez, E.; Fornaro, L.; Stafast, H.

    2005-05-15

    We employ femtosecond laser pulses (80 fs, 1.59 eV, and 80 MHz) to study the optical second-harmonic (SH) response of Pb{sub x}Cd{sub 1-x}Te ternary alloys (x about 0.2) grown by the vertical Bridgman method. The alloy segregates into a Pb-rich and a Cd-rich phase, the latter dominating the SH response of the ternary alloy by at least two orders of magnitude. Several sample regions show a regular layer-by-layer accommodation of the Pb-rich and Cd-rich phases as seen by a periodic alternation of the alloy's SH response on a {approx}10-{mu}m length scale. Furthermore, we employ polarization-resolved SH imaging as well as SH imaging at different azimuthal angles to obtain spatially resolved mappings of the sample, which are sensitive to the composition as well as the growth orientation of the Pb{sub x}Cd{sub 1-x}Te material system. We observe an azimuthal phase shift of approximately 30 deg. between coherent macroscopic regions (several mm{sup 2}) in the Cd-rich phase of the ternary alloy. We interpret these regions as large area crystalline grains of (111) and (411) crystal orientations and approximately equal composition. Hence, SH imaging is shown to spatially resolve regions of different growth directions within the Pb{sub x}Cd{sub 1-x}Te sample.

  13. Diagnostic potential of multimodal imaging of ovarian tissue using optical coherence tomography and second-harmonic generation microscopy

    PubMed Central

    Welge, Weston A.; DeMarco, Andrew T.; Watson, Jennifer M.; Rice, Photini S.; Barton, Jennifer K.; Kupinski, Matthew A.

    2014-01-01

    Abstract. Ovarian cancer is particularly deadly because it is usually diagnosed after it has metastasized. We have previously identified features of ovarian cancer using optical coherence tomography (OCT) and second-harmonic generation (SHG) microscopy (targeting collagen). OCT provides an image of the ovarian microstructure, while SHG provides a high-resolution map of collagen fiber bundle arrangement. Here, we investigated the diagnostic potential of dual-modality OCT and SHG imaging. We conducted a fully crossed, multireader, multicase study using seven human observers. Each observer classified 44 ex vivo mouse ovaries (16 normal and 28 abnormal) as normal or abnormal from OCT, SHG, and simultaneously viewed, coregistered OCT and SHG images and provided a confidence rating on a six-point scale. We determined the average receiver operating characteristic (ROC) curves, area under the ROC curves (AUC), and other quantitative figures of merit. The results show that OCT has diagnostic potential with an average AUC of 0.91±0.06. The average AUC for SHG was less promising at 0.71±0.13. The average AUC for simultaneous OCT and SHG was not significantly different from OCT alone, possibly due to the limited SHG field of view. The high performance of OCT and coregistered OCT and SHG warrants further investigation. PMID:25798444

  14. Measurement of lacunar bone strains and crack formation during tensile loading by digital volume correlation of second harmonic generation images.

    PubMed

    Wentzell, Scott; Nesbitt, Robert Sterling; Macione, James; Kotha, Shiva

    2016-07-01

    The maintenance of healthy bone tissue depends upon the ability of osteocytes to respond to mechanical cues on the cellular level. The combination of digital volume correlation and second harmonic generation microscopy offers the opportunity to investigate the mechanical microenvironment of intact bone on the scale of individual osteocytes. Adult human femurs were imaged under tensile loads of 5 and 15MPa and volumes of approximately 492×429×31μm(3) were analyzed, along with an image of a bone microcrack under the same loading conditions. Principal strains were significantly higher in three-dimensional digital volume correlation when compared to two-dimensional digital image correlation. The average maximum principal strain magnitude was 5.06-fold greater than the applied global strain, with peak strains of up to 23.14-fold over global strains measured at the borders of osteocyte lacunae. Finally, a microcrack that initiated at an osteocyte lacunae had its greatest tensile strain magnitudes at the crack expansion front in the direction of a second lacunae, but strain at the crack border was reduced to background strain magnitudes upon breaching the second lacunae. This serveed to demonstrate the role of lacunae in initiating, mediating and terminating microcrack growth. PMID:26807766

  15. Spectrally balanced detection for optical frequency domain imaging.

    PubMed

    Chen, Yueli; de Bruin, Daniel M; Kerbage, Charles; de Boer, Johannes F

    2007-12-10

    In optical frequency domain imaging (OFDI) or swept-source optical coherence tomography, balanced detection is required to suppress relative intensity noise (RIN). A regular implementation of balanced detection by combining reference and sample arm signal in a 50/50 coupler and detecting the differential output with a balanced receiver is however, not perfect. Since the splitting ratio of the 50/50 coupler is wavelength dependent, RIN is not optimally canceled at the edges of the wavelength sweep. The splitting ratio has a nearly linear shift of 0.4% per nanometer. This brings as much as +/-12% deviation at the margins of wavelength-swept range centered at 1060nm. We demonstrate a RIN suppression of 33dB by spectrally corrected balanced detection, 11dB more that regular balanced detection. PMID:19550929

  16. The role of tissue harmonic imaging ultrasound combined with power Doppler ultrasound in the diagnosis of childhood febrile urinary tract infections

    PubMed Central

    İlarslan, Nisa Eda Çullas; Fitöz, Ömer Suat; Öztuna, Derya Gökmen; Küçük, Nuriye Özlem; Yalçınkaya, Fatma Fatoş

    2015-01-01

    Aim: This study assessed the ability of tissue harmonic imaging ultrasound combined with power Doppler ultrasound in the detection of childhood febrile urinary tract infections in comparison with the gold standard reference method: Tc-99m dimercaptosuccinicacid renal cortical scintigraphy. Material and Methods: This prospective study included 60 patients who were hospitalized with a first episode of febrile urinary tract infections. All children were examined with dimercaptosuccinicacid scan and tissue harmonic imaging ultrasound combined with power Doppler ultrasound within the first 3 days of admission. Results: Signs indicative of acute infection were observed in 29 patients according to the results of tissue harmonic imaging ultrasound combined with power Doppler ultrasound while dimercaptosuccinicacid scan revealed abnormal findings in 33 patients. The sensitivity, specificity, positive predictive value and negative predictive value of tissue harmonic imaging combined with power Doppler ultrasound using dimercaptosuccinicacid scintigraphy as the reference method in patients diagnosed with first episode febrile urinary tract infections were calculated as 57.58% (95% confidence interval: 40.81%–72.76%); 62.96% (95% confidence interval: 44.23%–78.47%); 65.52% (95% confidence interval: 52.04%–77%); 54.84% (95% confidence interval: 41.54%–67.52%); respectively. Conclusions: Although current results exhibit inadequate success of power Doppler ultrasound, this practical and radiation-free method may soon be comprise a part of the routine ultrasonographic evaluation of febrile urinary tract infections of childhood if patients are evaluated early and under appropriate sedation. PMID:26265892

  17. Multi-parametric monitoring of high intensity focused ultrasound (HIFU) treatment using harmonic motion imaging for focused ultrasound (HMIFU)

    NASA Astrophysics Data System (ADS)

    Hou, Gary Y.; Marquet, Fabrice; Wang, Shutao; Konofagou, Elisa

    2012-11-01

    Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a recently developed high-intensity focused ultrasound (HIFU) treatment monitoring method with feasibilities demonstrated in vitro and in vivo. Here, a multi-parametric study is performed to investigate both elastic and acoustics-independent viscoelastic tissue changes using the Harmonic Motion Imaging (HMI) displacement, axial compressive strain and relative phase-shift during high energy HIFU where tissue boiling occurs. Forty three (n=18) thermal lesions were formed in ex vivo canine liver specimens. Two dimensional (2D) transverse HMI displacement maps were also obtained before and after lesion formation. The same method was repeated in 10-, 20-and 30-s HIFU durations at three different acoustic powers of 8, 10, and 11W. For the 10-, 20-, and 30-s treatment cases, a steady decrease in the displacement (-8.67±4.80, -14.44±7.77, 24.03±12.11μm), compressive strain -0.16±0.06, -0.71±0.30, -0.68±0.36 %, and phase shift +1.80±6.80, -15.80±9.44, -18.62±13.14 ° were obtained, respectively, indicating overall increase of relative stiffness and decrease of the viscosity-to-stiffness ratio during heating. After treatment, 2D HMI displacement images of the thermal lesions showed an increased lesion-to-background contrast of 1.34±0.19, 1.98±0.30, 2.26±0.80 and lesion size of 40.95±8.06, 47.6±4.87, and 52.23±2.19 mm2, respectively, which was validated again with pathology 25.17±6.99, 42.17±1.77, 47.17±3.10 mm2. Additionally, studies also investigated the performance of mutli-parametric monitoring under the influence of boiling and attenuation change due to tissue boiling, where discrepancies were found such as deteriorated displacement SNR and reversed lesion-to-background displacement contrast with indication on possible increase in attenuation and tissue gelatification or pulverization. Despite the challenge of the boiling mechanism, the relative phase shift served as consist biomechanical tissue

  18. Polarization dependence of aligned collagen tissues imaged with second harmonic generation microscopy.

    PubMed

    Ávila, Francisco J; Del Barco, Oscar; Bueno, Juan M

    2015-08-01

    A polarimetric second harmonic generation (SHG) microscope was used to analyze the dependence between polarization and SHG signal from collagen-based samples. A theoretical model was also developed to investigate the SHG intensity as a function of different polarization states for a set of quasiparallel fibers. Numerical simulations were compared to experimental SHG intensity values and a fairly good agreement was found. Linear polarized light produced periodical changes in the emitted SHG signal with a maximum of intensity corresponding to polarization parallel to the main orientation of the fibers, regardless the ratio of hyperpolarizabilities, ρρ . A similar behavior was found for elliptical states located along a vertical meridian on the Poincaré sphere (i.e., null azimuth) although the modulation of the SHG signal was different. Our numerical calculations described a dramatic change in this regular trend when ρρ changed from positive to negative values. Moreover, we provide an experimental method (based on the analysis of the modulation of the SHG signal) to determine the value of the ratio ρρ and, consequently, to obtain information about the internal organization of the collagen fibers. PMID:26263415

  19. Underground imaging by frequency-domain electromagnetic migration

    SciTech Connect

    Zhdanov, M.S.; Traynin, P.; Booker, J.R.

    1996-05-01

    A new method of the resistivity imaging based on frequency-domain electromagnetic migration is developed. Electromagnetic (EM) migration involves downward diffusion of observed EM fields whose time flow has been reversed. Unlike downward analytical continuation, migration is a stable procedure that accurately restores the phase of the upgoing field inside the Earth. This method is indented for the processing and interpretation of EM data collected for both TE and TM modes of plane-wave excitation. Until recently, the method could be applied only for determining the position of anomalous structures and for finding interfaces between layers of different conductivity. There were no well developed approaches to the resistivity imaging, which is the key problem in the inversion of EM data. The authors provide a novel approach to determining not only the position of anomalous structures but their resistivity as well. The main difficulty in the practical realization of this approach is determining the background resistivity distribution for migration. They discuss the method of the solution of this problem based on differential transformation of apparent resistivity curves. The final goal of migration is to provide a first order interpretation using a computational effort equivalent to a forward modeling calculation.

  20. Radio-frequency energy quantification in magnetic resonance imaging

    NASA Astrophysics Data System (ADS)

    Alon, Leeor

    Mapping of radio frequency (RF) energy deposition has been challenging for 50+ years, especially, when scanning patients in the magnetic resonance imaging (MRI) environment. As result, electromagnetic simulation software is often used for estimating the specific absorption rate (SAR), the rate of RF energy deposition in tissue. The thesis work presents challenges associated with aligning information provided by electromagnetic simulation and MRI experiments. As result of the limitations of simulations, experimental methods for the quantification of SAR were established. A system for quantification of the total RF energy deposition was developed for parallel transmit MRI (a system that uses multiple antennas to excite and image the body). The system is capable of monitoring and predicting channel-by-channel RF energy deposition, whole body SAR and capable of tracking potential hardware failures that occur in the transmit chain and may cause the deposition of excessive energy into patients. Similarly, we demonstrated that local RF power deposition can be mapped and predicted for parallel transmit systems based on a series of MRI temperature mapping acquisitions. Resulting from the work, we developed tools for optimal reconstruction temperature maps from MRI acquisitions. The tools developed for temperature mapping paved the way for utilizing MRI as a diagnostic tool for evaluation of RF/microwave emitting device safety. Quantification of the RF energy was demonstrated for both MRI compatible and non-MRI-compatible devices (such as cell phones), while having the advantage of being noninvasive, of providing millimeter resolution and high accuracy.

  1. Visible spatial frequency domain imaging with a digital light microprojector

    PubMed Central

    Lin, Alexander J.; Ponticorvo, Adrien; Konecky, Soren D.; Cui, Haotian; Rice, Tyler B.; Choi, Bernard; Durkin, Anthony J.

    2013-01-01

    Abstract. There is a need for cost effective, quantitative tissue spectroscopy and imaging systems in clinical diagnostics and pre-clinical biomedical research. A platform that utilizes a commercially available light-emitting diode (LED) based projector, cameras, and scaled Monte Carlo model for calculating tissue optical properties is presented. These components are put together to perform spatial frequency domain imaging (SFDI), a model-based reflectance technique that measures and maps absorption coefficients (μa) and reduced scattering coefficients (μs′) in thick tissue such as skin or brain. We validate the performance of the flexible LED and modulation element (FLaME) system at 460, 530, and 632 nm across a range of physiologically relevant μa values (0.07 to 1.5  mm−1) in tissue-simulating intralipid phantoms, showing an overall accuracy within 11% of spectrophotometer values for μa and 3% for μs′. Comparison of oxy- and total hemoglobin fits between the FLaME system and a spectrophotometer (450 to 1000 nm) is differed by 3%. Finally, we acquire optical property maps of a mouse brain in vivo with and without an overlying saline well. These results demonstrate the potential of FLaME to perform tissue optical property mapping in visible spectral regions and highlight how the optical clearing effect of saline is correlated to a decrease in μs′ of the skull. PMID:24005154

  2. Imaging Interplanetary CMEs at Radio Frequency From Solar Polar Orbit

    NASA Astrophysics Data System (ADS)

    Wu, Ji; Sun, Weiying; Zheng, Jianhua; Zhang, Cheng; Wang, Chi; Wang, C. B.; Wang, S.

    Coronal mass ejections (CMEs) are violent discharges of plasma and magnetic fields from the Sun's corona. They have come to be recognized as the major driver of physical conditions in the Sun-Earth system. Consequently, the detection of CMEs is important for un-derstanding and ultimately predicting space weather conditions. The Solar Polar Orbit Radio Telescope (SPORT) is a proposed mission to observe the propagation of interplanetary CMEs from solar polar orbit. The main payload (radio telescope) on board SPORT will be an in-terferometric imaging radiometer working at the meter wavelength band, which will follow the propagation of interplanetary CMEs from a distance of a few solar radii to near 1 AU from solar polar orbit. The SPORT spacecraft will also be equipped with a set of optical and in situ measurement instruments such as a EUV solar telescope, a solar wind plasma experiment, a solar wind ion composition instrument, an energetic particle detector, a wave detector, a mag-netometer and an interplanetary radio burst tracker. In this paper, we first describe the current shortage of interplanetary CME observations. Next, the scientific motivation and objectives of SPORT are introduced. We discuss the basic specifications of the main radio telescope of SPORT with reference to the radio emission mechanisms and the radio frequency band to be observed. Finally, we discuss the key technologies of the SPORT mission, including the con-ceptual design of the main telescope, the image retrieval algorithm and the solar polar orbit injection. Other payloads and their respective observation objectives are also briefly discussed. Key words: Interplanetary CMEs; Interferometric imaging; Solar polar orbit; Radiometer.

  3. Quantification of collagen fiber organization in biological tissues at cellular and molecular scales using second-harmonic generation imaging

    NASA Astrophysics Data System (ADS)

    Ambekar Ramachandra Rao, Raghu

    Collagen is the most abundant structural protein found in the human body, and is responsible for providing structure and function to tissues. Collagen molecules organize naturally into structures called fibers on the scale of the wavelength of light and lack inversion symmetry, thus allowing for the process of second harmonic generation (SHG) when exposed to intense incident light. We have developed two quantitative techniques: Fourier transform-second-harmonic generation (FT-SHG) imaging and generalized chi2 second-harmonic generation (chi2-SHG) imaging. In order to show that FT-SHG imaging can be used as a valuable diagnostic tool for real-world biological problems, we first investigate collagenase-induced injury in horse tendons. Clear differences in collagen fiber organization between normal and injured tendon are quantified. In particular, we observe that the regularly oriented organization of collagen fibers in normal tendons is disrupted in injured tendons leading to a more random organization. We also observe that FT-SHG microscopy is more sensitive in assessing tendon injury compared to the conventional polarized light microscopy. The second study includes quantifying collagen fibers in cortical bone using FT-SHG imaging and comparing it with scanning electron microscopy (SEM). Further, as an example study, we show how FT-SHG imaging could be used to quantify changes in bone structure as a function of age. Some initial work and future directions for extending FT-SHG to 3D are also discussed. The second technique, chi2-SHG imaging, takes advantage of the coherent nature of SHG and utilizes polarization to extract the second-order susceptibility (d elements) which provides information on molecular organization, i.e., it provides access to sub-diffractional changes "optically". We use chi2-SHG in combination with FT-SHG imaging to investigate a couple of biological problems. First, we quantify differences in collagen fiber organization between cornea and

  4. Angle correction for small animal tumor imaging with spatial frequency domain imaging (SFDI)

    PubMed Central

    Zhao, Yanyu; Tabassum, Syeda; Piracha, Shaheer; Nandhu, Mohan Sobhana; Viapiano, Mariano; Roblyer, Darren

    2016-01-01

    Spatial frequency domain imaging (SFDI) is a widefield imaging technique that allows for the quantitative extraction of tissue optical properties. SFDI is currently being explored for small animal tumor imaging, but severe imaging artifacts occur for highly curved surfaces (e.g. the tumor edge). We propose a modified Lambertian angle correction, adapted from the Minnaert correction method for satellite imagery, to account for tissue surface angles up to 75°. The method was tested in a hemisphere phantom study as well as a small animal tumor model. The proposed method reduced µa and µs` extraction errors by an average of 64% and 16% respectively compared to performing no angle correction, and provided more physiologically agreeable optical property and chromophore values on tumors. PMID:27375952

  5. High-frequency ultrasound annular array imaging. Part II: digital beamformer design and imaging.

    PubMed

    Hu, Chang-Hong; Snook, Kevin A; Cao, Pei-Jie; Shung, K Kirk

    2006-02-01

    This is the second part of a two-paper series reporting a recent effort in the development of a high-frequency annular array ultrasound imaging system. In this paper an imaging system composed of a six-element, 43 MHz annular array transducer, a six-channel analog front-end, a field programmable gate array (FPGA)-based beamformer, and a digital signal processor (DSP) microprocessor-based scan converter will be described. A computer is used as the interface for image display. The beamformer that applies delays to the echoes for each channel is implemented with the strategy of combining the coarse and fine delays. The coarse delays that are integer multiples of the clock periods are achieved by using a first-in-first-out (FIFO) structure, and the fine delays are obtained with a fractional delay (FD) filter. Using this principle, dynamic receiving focusing is achieved. The image from a wire phantom obtained with the imaging system was compared to that from a prototype ultrasonic backscatter microscope with a 45 MHz single-element transducer. The improved lateral resolution and depth of field from the wire phantom image were observed. Images from an excised rabbit eye sample also were obtained, and fine anatomical structures were discerned. PMID:16529105

  6. In vivo intracardiac OCT imaging through percutaneous access: towards image guided radio-frequency ablation

    NASA Astrophysics Data System (ADS)

    Wang, Hui; Kang, Wei; Carrigan, Thomas; Bishop, Austin; Rosenthal, Noah; Arruda, Mauricio; Rollins, Andrew M.

    2012-01-01

    BACKGROUND Complete catheter-tissue contact and permanent tissue destruction are essential for efficient radio-frequency ablation (RFA) during cardiac arrhythmia treatment. Current methods of monitoring lesion formation are indirect and unreliable. We aim to develop optical coherence tomography (OCT) as an imaging guidance for RFA. OBJECTIVES The purpose of this study is to evaluate the feasibility of using OCT catheter to image endocardia wall in active beating hearts through percutaneous access. This is a critical step toward image guided RFA in a clinic setting. METHODS A cone-scanning forward-viewing OCT catheter was advanced into active beating hearts through percutaneous access in four swine. The OCT catheter was steered by an introducer to touch the endocardia wall. The images were then acquired at 10 frames per second at an axial resolution and lateral resolution of 15 μm. RESULTS We report the first in vivo intracardiac OCT imaging through percutaneous access with a thin and flexible OCT catheter. We are able to acquire high quality OCT images in active beating hearts, observe the polarization-related artifacts induced by the birefringence of myocardium and readily evaluate catheter-tissue contact. CONCLUSIONS It is feasible to acquire OCT images in beating hearts through percutaneous access. The observations indicate that OCT could be a promising technique for in vivo guidance of RFA.

  7. Differential near-edge coherent diffractive imaging using a femtosecond high-harmonic XUV light source.

    PubMed

    Weise, Fabian; Neumark, Daniel M; Leone, Stephen R; Gessner, Oliver

    2012-11-19

    Element-specific contrast enhancement in tabletop coherent diffractive imaging (CDI) is demonstrated by employing an ultrafast extreme ultraviolet (XUV) light source with tunable photon energy. By combining two measurements performed at energies below and above the Al L(2,3) absorption edge, the spatial autocorrelation function of a micron-scale double pinhole in a 300 nm thick aluminum foil is retrieved despite a dominant background signal from directly transmitted light across the entire range of detectable diffraction angles. The fringe visibility in the diffraction patterns is 0 below the Al L(2,3) edge, 0.53 ± 0.06 above the edge, and 0.73 ± 0.08 in the differential image that combines the two measurements. The proof-of-principle experiment demonstrates that the variations of XUV optical constants in the vicinity of an inner-shell absorption edge can be utilized to improve the chemical sensitivity and image reconstruction quality of laboratory-based ultrafast imaging experiments. PMID:23187472

  8. Combined nonlinear laser imaging (two-photon excitation fluorescence, second and third-harmonic generation, and fluorescence lifetime imaging microscopies) in ovarian tumors

    NASA Astrophysics Data System (ADS)

    Adur, J.; Pelegati, V. B.; de Thomaz, A. A.; Bottcher-Luiz, F.; Andrade, L. A. L. A.; Almeida, D. B.; Carvalho, H. F.; Cesar, C. L.

    2012-03-01

    We applied Two-photon Excited Fluorescence (TPEF), Second/Third Harmonic Generation (SHG and THG) and Fluorescence Lifetime Imaging (FLIM) Non Linear Optics (NLO) Laser-Scanning Microscopy within the same imaging platform to evaluate their use as a diagnostic tool in ovarian tumors. We assess of applicability of this multimodal approach to perform a pathological evaluation of serous and mucinous tumors in human samples. The combination of TPEF-SHG-THG imaging provided complementary information about the interface epithelium/stromal, such as the transformation of epithelium surface (THG) and the overall fibrillar tissue architecture (SHG). The fact that H&E staining is the standard method used in clinical pathology and that the stored samples are usually fixed makes it important a re-evaluation of these samples with NLO microscopy to compare new results with a library of already existing samples. FLIM, however, depends on the chemical environment around the fluorophors that was completely changed after fixation; therefore it only makes sense in unstained samples. Our FLIM results in unstained samples demonstrate that it is possible to discriminate healthy epithelia from serous or mucinous epithelia. Qualitative and quantitative analysis of the different imaging modalities used showed that multimodal nonlinear microscopy has the potential to differentiate between cancerous and healthy ovarian tissue.

  9. Harmonic multiplication using resonant tunneling

    NASA Technical Reports Server (NTRS)

    Sollner, T. C. L. G.; Brown, E. R.; Goodhue, W. D.; Correa, C. A.

    1988-01-01

    This paper demonstrates the use of resonant-tunneling diodes as varistors for harmonic multiplication. It is shown that efficient odd-harmonic conversion is possible and that even harmonics do not appear because of the antisymmetry of the current-voltage (I-V) curve. It is also shown that, with the proper choice of resonant-tunneling structure and pump amplitude, most of the harmonic output power can be confined to a single odd-harmonic frequency. Fifth-harmonic multiplication was demonstrated with an output at 21.75 GHz and a power conversion efficiency of 0.5 percent, and a fifth-harmonic efficiency of 2.7 percent was achieved in a circuit simulation using an improved I-V curve.

  10. A novel technique of image quality objective measurement by wavelet analysis throughout the spatial frequency range

    NASA Astrophysics Data System (ADS)

    Luo, Gaoyong

    2005-01-01

    An essential determinant of the value of surrogate digital images is their quality. Image quality measurement has become crucial for most image processing applications. Over the past years , there have been many attempts to develop models or metrics for image quality that incorporate elements of human visual sensitivity. However, there is no current standard and objective definition of spectral image quality. This paper proposes a reliable automatic method for objective image quality measurement by wavelet analysis throughout the spatial frequency range. This is done by a detailed analysis of an image for a wide range of spatial frequency content, using a combination of modulation transfer function (MTF), brightness, contrast, saturation, sharpness and noise, as a more revealing metric for quality evaluation. A fast lifting wavelet algorithm is developed for computationally efficient spatial frequency analysis, where fine image detail corresponding to high spatial frequencies and image sharpness in regard to lower and mid -range spatial frequencies can be examined and compared accordingly. The wavelet frequency deconstruction is actually to extract the feature of edges in sub-band images. The technique provides a means to relate the quality of an image to the interpretation and quantification throughout the frequency range, in which the noise level is estimated in assisting with quality analysis. The experimental results of using this method for image quality measurement exhibit good correlation to subjective visual quality assessments.

  11. A novel technique of image quality objective measurement by wavelet analysis throughout the spatial frequency range

    NASA Astrophysics Data System (ADS)

    Luo, Gaoyong

    2004-10-01

    An essential determinant of the value of surrogate digital images is their quality. Image quality measurement has become crucial for most image processing applications. Over the past years , there have been many attempts to develop models or metrics for image quality that incorporate elements of human visual sensitivity. However, there is no current standard and objective definition of spectral image quality. This paper proposes a reliable automatic method for objective image quality measurement by wavelet analysis throughout the spatial frequency range. This is done by a detailed analysis of an image for a wide range of spatial frequency content, using a combination of modulation transfer function (MTF), brightness, contrast, saturation, sharpness and noise, as a more revealing metric for quality evaluation. A fast lifting wavelet algorithm is developed for computationally efficient spatial frequency analysis, where fine image detail corresponding to high spatial frequencies and image sharpness in regard to lower and mid -range spatial frequencies can be examined and compared accordingly. The wavelet frequency deconstruction is actually to extract the feature of edges in sub-band images. The technique provides a means to relate the quality of an image to the interpretation and quantification throughout the frequency range, in which the noise level is estimated in assisting with quality analysis. The experimental results of using this method for image quality measurement exhibit good correlation to subjective visual quality assessments.

  12. Quantitative Analysis of Vascular Heterogeneity in Breast Lesions Using Contrast-Enhanced 3-D Harmonic and Subharmonic Ultrasound Imaging

    PubMed Central

    Sridharan, Anush; Eisenbrey, John R.; Machado, Priscilla; Ojeda-Fournier, Haydee; Wilkes, Annina; Sevrukov, Alexander; Mattrey, Robert F.; Wallace, Kirk; Chalek, Carl L.; Thomenius, Kai E.; Forsberg, Flemming

    2015-01-01

    Ability to visualize breast lesion vascularity and quantify the vascular heterogeneity using contrast-enhanced 3-D harmonic (HI) and subharmonic (SHI) ultrasound imaging was investigated in a clinical population. Patients (n = 134) identified with breast lesions on mammography were scanned using power Doppler imaging, contrast-enhanced 3-D HI, and 3-D SHI on a modified Logiq 9 scanner (GE Healthcare). A region of interest corresponding to ultrasound contrast agent flow was identified in 4D View (GE Medical Systems) and mapped to raw slice data to generate a map of time-intensity curves for the lesion volume. Time points corresponding to baseline, peak intensity, and washout of ultrasound contrast agent were identified and used to generate and compare vascular heterogeneity plots for malignant and benign lesions. Vascularity was observed with power Doppler imaging in 84 lesions (63 benign and 21 malignant). The 3-D HI showed flow in 8 lesions (5 benign and 3 malignant), whereas 3-D SHI visualized flow in 68 lesions (49 benign and 19 malignant). Analysis of vascular heterogeneity in the 3-D SHI volumes found benign lesions having a significant difference in vascularity between central and peripheral sections (1.71 ± 0.96 vs. 1.13 ± 0.79 dB, p < 0.001, respectively), whereas malignant lesions showed no difference (1.66 ± 1.39 vs. 1.24 ± 1.14 dB, p = 0.24), indicative of more vascular coverage. These preliminary results suggest quantitative evaluation of vascular heterogeneity in breast lesions using contrast-enhanced 3-D SHI is feasible and able to detect variations in vascularity between central and peripheral sections for benign and malignant lesions. PMID:25935933

  13. Non-contact, ultrasound-based indentation method for measuring elastic properties of biological tissues using Harmonic Motion Imaging (HMI)

    NASA Astrophysics Data System (ADS)

    Vappou, Jonathan; Hou, Gary Y.; Marquet, Fabrice; Shahmirzadi, Danial; Grondin, Julien; Konofagou, Elisa E.

    2015-04-01

    Noninvasive measurement of mechanical properties of biological tissues in vivo could play a significant role in improving the current understanding of tissue biomechanics. In this study, we propose a method for measuring elastic properties non-invasively by using internal indentation as generated by harmonic motion imaging (HMI). In HMI, an oscillating acoustic radiation force is produced by a focused ultrasound transducer at the focal region, and the resulting displacements are estimated by tracking radiofrequency signals acquired by an imaging transducer. In this study, the focal spot region was modeled as a rigid cylindrical piston that exerts an oscillatory, uniform internal force to the underlying tissue. The HMI elastic modulus EHMI was defined as the ratio of the applied force to the axial strain measured by 1D ultrasound imaging. The accuracy and the precision of the EHMI estimate were assessed both numerically and experimentally in polyacrylamide tissue-mimicking phantoms. Initial feasibility of this method in soft tissues was also shown in canine liver specimens in vitro. Very good correlation and agreement was found between the measured Young’s modulus and the HMI modulus in the numerical study (r2 > 0.99, relative error <10%) and on polyacrylamide gels (r2 = 0.95, relative error <24%). The average HMI modulus on five liver samples was found to EHMI = 2.62  ±  0.41 kPa, compared to EMechTesting = 4.2  ±  2.58 kPa measured by rheometry. This study has demonstrated for the first time the initial feasibility of a non-invasive, model-independent method to estimate local elastic properties of biological tissues at a submillimeter scale using an internal indentation-like approach. Ongoing studies include in vitro experiments in a larger number of samples and feasibility testing in in vivo models as well as pathological human specimens.

  14. Non-contact, Ultrasound-based Indentation Method for Measuring Elastic Properties of Biological Tissues Using Harmonic Motion Imaging (HMI)

    PubMed Central

    Vappou, Jonathan; Hou, Gary Y.; Marquet, Fabrice; Shahmirzadi, Danial; Grondin, Julien; Konofagou, Elisa E.

    2015-01-01

    Noninvasive measurement of mechanical properties of biological tissues in vivo could play a significant role in improving the current understanding of tissue biomechanics. In this study, we propose a method for measuring elastic properties non-invasively by using internal indentation as generated by Harmonic Motion Imaging (HMI). In HMI, an oscillating acoustic radiation force is produced by a focused ultrasound transducer at the focal region, and the resulting displacements are estimated by tracking RF signals acquired by an imaging transducer. In this study, the focal spot region was modeled as a rigid cylindrical piston that exerts an oscillatory, uniform internal force to the underlying tissue. The HMI elastic modulus EHMI was defined as the ratio of the applied force to the axial strain measured by 1D ultrasound imaging. The accuracy and the precision of the EHMI estimate were assessed both numerically and experimentally in polyacrylamide tissue-mimicking phantoms. Initial feasibility of this method in soft tissues was also shown in canine liver specimens in vitro. Very good correlation and agreement was found between the actual Young’s modulus and the HMI modulus in the numerical study (r2>0.99, relative error <10%) and on polyacrylamide gels (r2=0.95, relative error <24%). The average HMI modulus on five liver samples was found to EHMI=2.62±0.41 kPa, compared to EMechTesting=4.2±2.58 kPa measured by rheometry. This study has demonstrated for the first time the initial feasibility of a non-invasive, model-independent method to estimate local elastic properties of biological tissues at a submillimeter scale using an internal indentation-like approach. Ongoing studies include in vitro experiments in a larger number of samples and feasibility testing in in vivo models as well as pathological human specimens. PMID:25776065

  15. Advanced Reservoir Imaging Using Frequency-Dependent Seismic Attributes

    SciTech Connect

    Fred Hilterman; Tad Patzek; Gennady Goloshubin; Dmitriy Silin; Charlotte Sullivan; Valeri Korneev

    2007-12-31

    Our report concerning advanced imaging and interpretation technology includes the development of theory, the implementation of laboratory experiments and the verification of results using field data. We investigated a reflectivity model for porous fluid-saturated reservoirs and demonstrated that the frequency-dependent component of the reflection coefficient is asymptotically proportional to the reservoir fluid mobility. We also analyzed seismic data using different azimuths and offsets over physical models of fractures filled with air and water. By comparing our physical model synthetics to numerical data we have identified several diagnostic indicators for quantifying the fractures. Finally, we developed reflectivity transforms for predicting pore fluid and lithology using rock-property statistics from 500 reservoirs in both the shelf and deep-water Gulf of Mexico. With these transforms and seismic AVO gathers across the prospect and its down-dip water-equivalent reservoir, fluid saturation can be estimated without a calibration well that ties the seismic. Our research provides the important additional mechanisms to recognize, delineate, and validate new hydrocarbon reserves and assist in the development of producing fields.

  16. Imaging the Subduction Plate Interface Using Low-Frequency Earthquakes

    NASA Astrophysics Data System (ADS)

    Plourde, A. P.; Bostock, M. G.

    2015-12-01

    Low-frequency Earthquakes (LFEs) in subduction zones are commonly thought to represent slip on the plate interface. They have also been observed to lie near or within a zone of low shear-wave velocity, which is modelled as fluid-rich upper oceanic crust. Due to relatively large depth uncertainties in absolute hypocenters of most LFE families, their location relative to an independently imaged subucting plate and, consequently, the nature of the plate boundary at depths between 30-45 km have not been precisely determined. For a selection of LFE families in northern Washington, we measure variations in arrival time of individual LFE detections using multi-channel cross-correlation incorporating both arrivals at the same station and different events (cross-detection data), and the same event but different stations (cross-station data). Employing HypoDD, these times are used to generate relative locations for individual LFE detections. After creating templates from spatial subgroups of detections, network cross-correlation techniques will be used to search for new detections in neighbouring areas, thereby expanding the local catalogue and enabling further subdivision. By combining the source ``arrays'' and the receiver arrays from the Array of Arrays experiment we plan to interrogate plate boundary structure using migration of scattered waves from the subduction complex as previously documented beneath southern Vancouver Island.

  17. Three Dimensional Speckle Imaging Employing a Frequency-Locked Tunable Diode Laser

    SciTech Connect

    Cannon, Bret D.; Bernacki, Bruce E.; Schiffern, John T.; Mendoza, Albert

    2015-09-01

    We describe a high accuracy frequency stepping method for a tunable diode laser to improve a three dimensional (3D) imaging approach based upon interferometric speckle imaging. The approach, modeled after Takeda, exploits tuning an illumination laser in frequency as speckle interferograms of the object (specklegrams) are acquired at each frequency in a Michelson interferometer. The resulting 3D hypercube of specklegrams encode spatial information in the x-y plane of each image with laser tuning arrayed along its z-axis. We present laboratory data of before and after results showing enhanced 3D imaging resulting from precise laser frequency control.

  18. Analytical Harmonic Vibrational Frequencies for the Green Fluorescent Protein Computed with ONIOM: Chromophore Mode Character and Its Response to Environment.

    PubMed

    Thompson, Lee M; Lasoroski, Aurélie; Champion, Paul M; Sage, J Timothy; Frisch, Michael J; van Thor, Jasper J; Bearpark, Michael J

    2014-02-11

    A systematic comparison of different environmental effects on the vibrational modes of the 4-hydroxybenzylidene-2,3-dimethylimidazolinone (HBDI) chromophore using the ONIOM method allows us to model how the molecule's spectroscopic transitions are modified in the Green Fluorescent Protein (GFP). ONIOM(QM:MM) reduces the expense of normal mode calculations when computing the majority of second derivatives only at the MM level. New developments described here for the efficient solution of the CPHF equations, including contributions from electrostatic interactions with environment charges, mean that QM model systems of ∼100 atoms can be embedded within a much larger MM environment of ∼5000 atoms. The resulting vibrational normal modes, their associated frequencies, and dipole derivative vectors have been used to interpret experimental difference spectra (GFPI2-GFPA), chromophore vibrational Stark shifts, and changes in the difference between electronic and vibrational transition dipoles (mode angles) in the protein environment. PMID:26580050

  19. A real-time algorithm for the harmonic estimation and frequency tracking of dominant components in fusion plasma magnetic diagnostics

    SciTech Connect

    Alves, D.; Coelho, R. [Associação Euratom Collaboration: JET-EFDA Contributors

    2013-08-15

    The real-time tracking of instantaneous quantities such as frequency, amplitude, and phase of components immerse in noisy signals has been a common problem in many scientific and engineering fields such as power systems and delivery, telecommunications, and acoustics for the past decades. In magnetically confined fusion research, extracting this sort of information from magnetic signals can be of valuable assistance in, for instance, feedback control of detrimental magnetohydrodynamic modes and disruption avoidance mechanisms by monitoring instability growth or anticipating mode-locking events. This work is focused on nonlinear Kalman filter based methods for tackling this problem. Similar methods have already proven their merits and have been successfully employed in this scientific domain in applications such as amplitude demodulation for the motional Stark effect diagnostic. In the course of this work, three approaches are described, compared, and discussed using magnetic signals from the Joint European Torus tokamak plasma discharges for benchmarking purposes.

  20. Effects of density functionals and dispersion interactions on geometries, bond energies and harmonic frequencies of Etbnd UX3 (E = N, P, CH; X = H, F, Cl)

    NASA Astrophysics Data System (ADS)

    Pandey, Krishna Kumar; Patidar, Pankaj; Patidar, Sunil Kumar; Vishwakarma, Ravi

    2014-12-01

    Quantum-chemical calculations have been performed to evaluate the geometries, bonding nature and harmonic frequencies of the compounds [Etbnd UX3] at DFT, DFT-D3, DFT-D3(BJ) and DFT-dDSc levels using different density functionals BP86, BLYP, PBE, revPBE, PW91, TPSS and M06-L. The stretching frequency of Utbnd N bond in [Ntbnd UF3] calculated with DFT/BLYP closely resembles with the experimental value. The performance of different density functionals for accurate Utbnd N vibrational frequencies follows the order BLYP > revPBE > BP86 > PW91 > TPSS > PBE > M06-L. The BLYP functional gives accurate value of the Utbnd E bond distances. The uranium atom in the studied compounds [Etbnd UX3] is positively charged. Upon going from [Etbnd UF3] to [Etbnd UCl3], the partial Hirshfeld charge on uranium atom decreases because of the lower electronegativity of chlorine compared to flourine. The Gopinathan-Jug bond order for Utbnd E bonds ranges from 2.90 to 3.29. The Utbnd E bond dissociation energies vary with different density functionals as M06-L < TPSS < BLYP < revPBE < BP86 < PBE ≈ PW91. The orbital interactions ΔEorb, in all studied compounds [Etbnd UX3] are larger than the electrostatic interaction ΔEelstat, which means the Utbnd N bonds in these compound have greater degree of covalent character (in the range 63.8-77.2%). The Usbnd E σ-bonding interaction is the dominant bonding interaction in the nitride and methylidyne complexes while it is weaker in [Ptbnd UX3]. The dispersion energy contributions to the total bond dissociation energies are rather small. Compared to the Grimme's D3(BJ) corrections, the Corminboeuf's dispersion corrections are larger with metaGGA functionals (TPSS, M06-L) while smaller with GGA functionals.

  1. Joint time-frequency analysis of high-bandwidth low-resolution ISAR imaging systems

    NASA Astrophysics Data System (ADS)

    Ghogomu, Patrick; Testorf, Markus E.

    2003-09-01

    Joint time-frequency analysis is applied to radar imaging problems. Special attention is given to imaging applications, for which the resolution is severely limited due the available bandwidth of the radar signal both in range and cross-range. This includes the detection of landmines as well as foliage penetration radar imaging. Motivated by this type of imaging problem a new joint time-frequency method, the STPDFT algorithm is introduced and compared with existing methods. The performance of all methods is illustrated with synthetic test signals. In addition, preliminary results are presented which demonstrate the performance of joint-time frequency transforms, if applied to low resolution imaging problems.

  2. High-intensity focused ultrasound monitoring using harmonic motion imaging for focused ultrasound (HMIFU) under boiling or slow denaturation conditions.

    PubMed

    Hou, Gary Y; Marquet, Fabrice; Wang, Shutao; Apostolakis, Iason-Zacharias; Konofagou, Elisa E

    2015-07-01

    Harmonic motion imaging for focused ultrasound (HMIFU) is a recently developed high-intensity focused ultrasound (HIFU) treatment monitoring method that utilizes an amplitude-modulated therapeutic ultrasound beam to induce an oscillatory radiation force at the HIFU focus and estimates the focal tissue displacement to monitor the HIFU thermal treatment. In this study, the performance of HMIFU under acoustic, thermal, and mechanical effects was investigated. The performance of HMIFU was assessed in ex vivo canine liver specimens (n = 13) under slow denaturation or boiling regimes. A passive cavitation detector (PCD) was used to assess the acoustic cavitation activity, and a bare-wire thermocouple was used to monitor the focal temperature change. During lesioning with slow denaturation, high quality displacements (correlation coefficient above 0.97) were observed under minimum cavitation noise, indicating the tissue initial-softening-then- stiffening property change. During HIFU with boiling, HMIFU monitored a consistent change in lesion-to-background displacement contrast (0.46 ± 0.37) despite the presence of strong cavitation noise due to boiling during lesion formation. Therefore, HMIFU effectively monitored softening-then-stiffening during lesioning under slow denaturation, and detected lesioning under boiling with a distinct change in displacement contrast under boiling in the presence of cavitation. In conclusion, HMIFU was shown under both boiling and slow denaturation regimes to be effective in HIFU monitoring and lesioning identification without being significantly affected by cavitation noise. PMID:26168177

  3. RETRACTED — Studies on the effect of instability of divergence, pointing and amplitude of green and yellow radiation pulses of copper vapour laser in second harmonic and sum frequency conversion

    NASA Astrophysics Data System (ADS)

    Prakash, Om; Mahakud, Ramakanta; Nakhe, Shankar V.; Dixit, Sudhir K.

    2013-02-01

    This paper presents the effect of single pulse stability of divergence angle, beam pointing angle and amplitude of green and yellow radiation pulses of an unstable resonator copper vapour laser (CVL) oscillator in the sum frequency mixing and second harmonic. The conversion efficiency of sum frequency generation was lower compared to second harmonic processes despite larger fundamental power being used in sum frequency experiments. However the net UV power obtained at the sum frequency was higher than both of the second harmonic UV frequencies. Lower SFG conversion efficiency (12.4%—271 nm) compared to SHG (16.7%—255 nm, 14.5%—289 nm) of individual CVL radiations is attributed to difference in single pulse stability of beam pointing, divergence and amplitude fluctuation of both CVL radiations in addition to commonly known fact of spatio-temporal mis-match. At the same fundamental input power (2.7 W), higher SH conversion efficiency of yellow (12.7%) compared to green (11.0%) is attributed to its better single pulse stability of beam pointing and divergence.

  4. Dual aperture dipole magnet with second harmonic component

    DOEpatents

    Praeg, W.F.

    1983-08-31

    An improved dual aperture dipole electromagnet includes a second-harmonic frequency magnetic guide field winding which surrounds first harmonic frequency magnetic guide field windings associated with each aperture. The second harmonic winding and the first harmonic windings cooperate to produce resultant magnetic waveforms in the apertures which have extended acceleration and shortened reset portions of electromagnet operation.

  5. Dual aperture dipole magnet with second harmonic component

    DOEpatents

    Praeg, Walter F.

    1985-01-01

    An improved dual aperture dipole electromagnet includes a second-harmonic frequency magnetic guide field winding which surrounds first harmonic frequency magnetic guide field windings associated with each aperture. The second harmonic winding and the first harmonic windings cooperate to produce resultant magnetic waveforms in the apertures which have extended acceleration and shortened reset portions of electromagnet operation.

  6. Continuous-wave, single-pass, single-frequency second-harmonic-generation at 266 nm based on birefringent-multicrystal scheme.

    PubMed

    Devi, Kavita; Parsa, S; Ebrahim-Zadeh, M

    2016-04-18

    We report the implementation of a compact cascaded multicrystal scheme based on birefringent crystals in critical phase-matching, for the generation of continuous-wave (cw) radiation in the deep ultraviolet (UV). The approach comprises a cascade of 4 single-pass second-harmonic-generation (SHG) stages in β-BaB2O4 (BBO) pumped by a single-frequency cw green source at 532 nm. A deep-UV cw output power of 37.7 mW at 266 nm has been obtained with a high passive power stability of 0.12% rms over more than 4 hours. Characterization and optimization of the system in each stage has been systematically performed. Angular phase-matching acceptance bandwidth under tight focusing in BBO, and spectral properties of the deep-UV radiation, have been studied. Theoretical calculations for SHG in the cascaded scheme based on birefringent phase-matching have been performed, and enhancement in UV power compared to single-stage single-pass scheme are studied. Theoretical comparison of BBO with other potential crystals for deep-UV generation in cascaded multicrystal scheme is also presented. PMID:27137310

  7. Sparse matrix beamforming and image reconstruction for real-time 2D HIFU monitoring using Harmonic Motion Imaging for Focused Ultrasound (HMIFU) with in vitro validation

    PubMed Central

    Hou, Gary Y.; Provost, Jean; Grondin, Julien; Wang, Shutao; Marquet, Fabrice; Bunting, Ethan; Konofagou, Elisa E.

    2015-01-01

    Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a recently developed High-Intensity Focused Ultrasound (HIFU) treatment monitoring method. HMIFU utilizes an Amplitude-Modulated (fAM = 25 Hz) HIFU beam to induce a localized focal oscillatory motion, which is simultaneously estimated and imaged by confocally-aligned imaging transducer. HMIFU feasibilities have been previously shown in silico, in vitro, and in vivo in 1-D or 2-D monitoring of HIFU treatment. The objective of this study is to develop and show the feasibility of a novel fast beamforming algorithm for image reconstruction using GPU-based sparse-matrix operation with real-time feedback. In this study, the algorithm was implemented onto a fully integrated, clinically relevant HMIFU system composed of a 93-element HIFU transducer (fcenter = 4.5MHz) and coaxially-aligned 64-element phased array (fcenter = 2.5MHz) for displacement excitation and motion estimation, respectively. A single transmit beam with divergent beam transmit was used while fast beamforming was implemented using a GPU-based delay-and-sum method and a sparse-matrix operation. Axial HMI displacements were then estimated from the RF signals using a 1-D normalized cross-correlation method and streamed to a graphic user interface. The present work developed and implemented a sparse matrix beamforming onto a fully-integrated, clinically relevant system, which can stream displacement images up to 15 Hz using a GPU-based processing, an increase of 100 fold in rate of streaming displacement images compared to conventional CPU-based conventional beamforming and reconstruction processing. The achieved feedback rate is also currently the fastest and only approach that does not require interrupting the HIFU treatment amongst the acoustic radiation force based HIFU imaging techniques. Results in phantom experiments showed reproducible displacement imaging, and monitoring of twenty two in vitro HIFU treatments using the new 2D system showed a

  8. Eddy current imaging with an atomic radio-frequency magnetometer

    NASA Astrophysics Data System (ADS)

    Wickenbrock, Arne; Leefer, Nathan; Blanchard, John W.; Budker, Dmitry

    2016-05-01

    We use a radio-frequency 85Rb alkali-vapor cell magnetometer based on a paraffin-coated cell with long spin-coherence time and a small, low-inductance driving coil to create highly resolved conductivity maps of different objects. We resolve sub-mm features in conductive objects, we characterize the frequency response of our technique, and by operating at frequencies up to 250 kHz we are able to discriminate between differently conductive materials based on the induced response. The method is suited to cover a wide range of driving frequencies and can potentially be used for detecting non-metallic objects with low DC conductivity.

  9. Co-registration of ultrasound and frequency-domain photoacoustic radar images and image improvement for tumor detection

    NASA Astrophysics Data System (ADS)

    Dovlo, Edem; Lashkari, Bahman; Choi, Sung soo Sean; Mandelis, Andreas

    2015-03-01

    This paper demonstrates the co-registration of ultrasound (US) and frequency domain photoacoustic radar (FD-PAR) images with significant image improvement from applying image normalization, filtering and amplification techniques. Achieving PA imaging functionality on a commercial Ultrasound instrument could accelerate clinical acceptance and use. Experimental results presented demonstrate live animal testing and show enhancements in signal-to-noise ratio (SNR), contrast and spatial resolution. The co-registered image produced from the US and phase PA images, provides more information than both images independently.

  10. Multibeam single frequency synthetic aperture radar processor for imaging separate range swaths

    NASA Technical Reports Server (NTRS)

    Jain, A. (Inventor)

    1982-01-01

    A single-frequency multibeam synthetic aperture radar for large swath imaging is disclosed. Each beam illuminates a separate ""footprint'' (i.e., range and azimuth interval). The distinct azimuth intervals for the separate beams produce a distinct Doppler frequency spectrum for each beam. After range correlation of raw data, an optical processor develops image data for the different beams by spatially separating the beams to place each beam of different Doppler frequency spectrum in a different location in the frequency plane as well as the imaging plane of the optical processor. Selection of a beam for imaging may be made in the frequency plane by adjusting the position of an aperture, or in the image plane by adjusting the position of a slit. The raw data may also be processed in digital form in an analogous manner.

  11. A compact frequency-domain photon migration system for integration into commercial hybrid small animal imaging scanners for fluorescence tomography.

    PubMed

    Darne, Chinmay D; Lu, Yujie; Tan, I-Chih; Zhu, Banghe; Rasmussen, John C; Smith, Anne M; Yan, Shikui; Sevick-Muraca, Eva M

    2012-12-21

    The work presented herein describes the system design and performance evaluation of a miniaturized near-infrared fluorescence (NIRF) frequency-domain photon migration (FDPM) system with non-contact excitation and homodyne detection capability for small animal fluorescence tomography. The FDPM system was developed specifically for incorporation into a Siemens micro positron emission tomography/computed tomography (microPET/CT) commercial scanner for hybrid small animal imaging, but could be adapted to other systems. Operating at 100 MHz, the system noise was minimized and the associated amplitude and phase errors were characterized to be ±0.7% and ±0.3°, respectively. To demonstrate the tomographic ability, a commercial mouse-shaped phantom with 50 µM IRDye800CW and ⁶⁸Ga containing inclusion was used to associate PET and NIRF tomography. Three-dimensional mesh generation and anatomical referencing was accomplished through CT. A third-order simplified spherical harmonics approximation (SP₃) algorithm, for efficient prediction of light propagation in small animals, was tailored to incorporate the FDPM approach. Finally, the PET-NIRF target co-localization accuracy was analyzed in vivo with a dual-labeled imaging agent targeting orthotopic growth of human prostate cancer. The obtained results validate the integration of time-dependent fluorescence tomography system within a commercial microPET/CT scanner for multimodality small animal imaging. PMID:23171509

  12. A compact frequency-domain photon migration system for integration into commercial hybrid small animal imaging scanners for fluorescence tomography

    NASA Astrophysics Data System (ADS)

    Darne, Chinmay D.; Lu, Yujie; Tan, I.-Chih; Zhu, Banghe; Rasmussen, John C.; Smith, Anne M.; Yan, Shikui; Sevick-Muraca, Eva M.

    2012-12-01

    The work presented herein describes the system design and performance evaluation of a miniaturized near-infrared fluorescence (NIRF) frequency-domain photon migration (FDPM) system with non-contact excitation and homodyne detection capability for small animal fluorescence tomography. The FDPM system was developed specifically for incorporation into a Siemens micro positron emission tomography/computed tomography (microPET/CT) commercial scanner for hybrid small animal imaging, but could be adapted to other systems. Operating at 100 MHz, the system noise was minimized and the associated amplitude and phase errors were characterized to be ±0.7% and ±0.3°, respectively. To demonstrate the tomographic ability, a commercial mouse-shaped phantom with 50 µM IRDye800CW and 68Ga containing inclusion was used to associate PET and NIRF tomography. Three-dimensional mesh generation and anatomical referencing was accomplished through CT. A third-order simplified spherical harmonics approximation (SP3) algorithm, for efficient prediction of light propagation in small animals, was tailored to incorporate the FDPM approach. Finally, the PET-NIRF target co-localization accuracy was analyzed in vivo with a dual-labeled imaging agent targeting orthotopic growth of human prostate cancer. The obtained results validate the integration of time-dependent fluorescence tomography system within a commercial microPET/CT scanner for multimodality small animal imaging.

  13. A compact frequency-domain photon migration system for integration into commercial hybrid small animal imaging scanners for fluorescence tomography

    PubMed Central

    Darne, Chinmay D.; Lu, Yujie; Tan, I-Chih; Zhu, Banghe; Rasmussen, John C.; Smith, Anne M.; Yan, Shikui; Sevick-Muraca, Eva M

    2012-01-01

    The work presented herein describes system design and performance evaluation of a miniaturized near-infrared fluorescence (NIRF) frequency-domain photon migration (FDPM) system with non-contact excitation and homodyne detection capability for small animal fluorescence tomography. The FDPM system was developed specifically for incorporation into a Siemens microPET/CT commercial scanner for hybrid small animal imaging, but could be adapted to other systems. Operating at 100 MHz, the system noise was minimized and the associated amplitude and phase errors were characterized to be ±0.7% and ±0.3°, respectively. To demonstrate the tomographic ability, a commercial mouse-shaped phantom with 50 μM IRDye800CW and 68Ga containing inclusion was used to associate PET and NIRF tomography. 3-D mesh generation and anatomical referencing was accomplished through CT. A simplified spherical harmonics approximation (SP3) algorithm, for efficient prediction of light propagation in small animals, was tailored to incorporate FDPM approach. Finally, PET-NIRF target co-localization accuracy was analyzed in vivo with a dual-labeled imaging agent targeting orthotopic growth of human prostate cancer. The results obtained validate the integration of time-dependent fluorescence tomography system within a commercial microPET/CT scanner for multimodality small animal imaging. PMID:23171509

  14. Investigating ultrasound imaging in the frequency domain for tissue characterisation

    NASA Astrophysics Data System (ADS)

    Stromer, Jeremy; Ladani, Leila

    2016-07-01

    The potential of ultrasound imaging for use in distinguishing structures present in soft materials is investigated. In this study, images were reconstructed using non-standard parameters, which have been shown to vary according to different tissue structures. Due to the previously determined dependence on material microstructure, we investigate the possibility of these parameters as a basis for imaging soft materials. The feasibility of imaging methods was first tested on a large scale using 0.5-MHz ultrasound transducers. Imaging was then extended to a smaller scale using small-diameter 25-MHz transducers. The resulting images were compared to conventional C-scans with minimal data processing and were found to be of at least similar quality. These initial results show the possibility of using nonconventional ultrasound measurements as another means of imaging tissue and other soft materials for the presence of internal inclusions.

  15. A Simplified Sum-Frequency Vibrational Imaging Setup Used for Imaging Lipid Bilayer Arrays

    PubMed Central

    Smith, Kathryn A.; Conboy, John C.

    2012-01-01

    Given the complexity of cell membranes, there is a need for an analytical technique which can explore the physical properties of lipid membranes in a high-throughput and noninvasive manner. A simplified sum-frequency vibrational imaging (SFVI) setup has been developed and characterized using asymmetrically prepared 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC):1,2-distearoyl(d70)-sn-glycero-3-phosphocholine (DSPC-d70) lipid bilayer arrays. Exploiting the vibrational selectivity and inherent symmetry constraints of sum-frequency generation, SFVI was successfully used to probe the transition temperature of a patterned DSPC:DSPC-d70 lipid bilayer array. SFVI was also used to study the phase behavior in a multi-component micropatterned lipid bilayer array (MLBA) prepared using three different binary lipid mixtures (1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC):DSPC, DOPC:1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC:DSPC). This paper demonstrates that a simplified SFVI setup provides the necessary chemical imaging capabilities with the spatial resolution, sensitivity and field of view required for exploring lipid membrane properties in a high-throughput array based assay. PMID:22947074

  16. A simplified sum-frequency vibrational imaging setup used for imaging lipid bilayer arrays.

    PubMed

    Smith, Kathryn A; Conboy, John C

    2012-10-01

    Given the complexity of cell membranes, there is a need for an analytical technique which can explore the physical properties of lipid membranes in a high-throughput and noninvasive manner. A simplified sum-frequency vibrational imaging (SFVI) setup has been developed and characterized using asymmetrically prepared 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC):1,2-distearoyl(d70)-sn-glycero-3-phosphocholine (DSPC-d(70)) lipid bilayer arrays. Exploiting the vibrational selectivity and inherent symmetry constraints of sum-frequency generation, SFVI was successfully used to probe the transition temperature of a patterned DSPC:DSPC-d(70) lipid bilayer array. SFVI was also used to study the phase behavior in a multicomponent micropatterned lipid bilayer array (MLBA) prepared using three different binary lipid mixtures (1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC):DSPC, DOPC:1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC:DSPC). This paper demonstrates that a simplified SFVI setup provides the necessary chemical imaging capabilities with the spatial resolution, sensitivity, and field of view required for exploring lipid membrane properties in a high-throughput array based assay. PMID:22947074

  17. Two-photon spectral fluorescence lifetime and second-harmonic generation imaging of the porcine cornea with a 12-femtosecond laser microscope

    NASA Astrophysics Data System (ADS)

    Batista, Ana; Breunig, Hans Georg; Uchugonova, Aisada; Morgado, António Miguel; König, Karsten

    2016-03-01

    Five dimensional microscopy with a 12-fs laser scanning microscope based on spectrally resolved two-photon autofluorescence lifetime and second-harmonic generation (SHG) imaging was used to characterize all layers of the porcine cornea. This setup allowed the simultaneous excitation of both metabolic cofactors, NAD(P)H and flavins, and their discrimination based on their spectral emission properties and fluorescence decay characteristics. Furthermore, the architecture of the stromal collagen fibrils was assessed by SHG imaging in both forward and backward directions. Information on the metabolic state and the tissue architecture of the porcine cornea were obtained with subcellular resolution, and high temporal and spectral resolutions.

  18. Imaging of dielectric cylinders from experimental stepped-frequency data

    NASA Astrophysics Data System (ADS)

    Dubois, Anthony; Geffrin, Jean-Michel; Belkebir, Kamal; Saillard, Marc

    2006-04-01

    This letter reports on the characterization of two-dimensional targets from measured transient scattered fields. The problem is formulated in the frequency domain rather than directly in the time domain, and an iterative construction of the unknown dielectric constant is derived. Improvement of the resolution is suggested by marching on in the central frequency of the incident pulse. The efficiency of this approach is emphasized through examples of reconstructions of an inhomogeneous target from experimental data.

  19. A novel dual-frequency imaging method for intravascular ultrasound applications.

    PubMed

    Qiu, Weibao; Chen, Yan; Wong, Chi-Man; Liu, Baoqiang; Dai, Jiyan; Zheng, Hairong

    2015-03-01

    Intravascular ultrasound (IVUS), which is able to delineate internal structures of vessel wall with fine spatial resolution, has greatly enriched the knowledge of coronary atherosclerosis. A novel dual-frequency imaging method is proposed in this paper for intravascular imaging applications. A probe combined two ultrasonic transducer elements with different center frequencies (36 MHz and 78 MHz) is designed and fabricated with PMN-PT single crystal material. It has the ability to balance both imaging depth and resolution, which are important imaging parameters for clinical test. A dual-channel imaging platform is also proposed for real-time imaging, and this platform has been proven to support programmable processing algorithms, flexible imaging control, and raw RF data acquisition for IVUS applications. Testing results show that the -6 dB axial and lateral imaging resolutions of low-frequency ultrasound are 78 and 132 μm, respectively. In terms of high-frequency ultrasound, axial and lateral resolutions are determined to be as high as 34 and 106 μm. In vitro intravascular imaging on healthy swine aorta is conducted to demonstrate the performance of the dual-frequency imaging method for IVUS applications. PMID:25454093

  20. Harmonic uniflow engine

    DOEpatents

    Bennett, Charles L.

    2016-03-22

    A reciprocating-piston uniflow engine includes a harmonic oscillator inlet valve capable of oscillating at a resonant frequency for controlling the flow of working fluid into the engine. In particular, the inlet valve includes an inlet valve head and a spring arranged together as a harmonic oscillator so that the inlet valve head is moveable from an unbiased equilibrium position to a biased closed position occluding an inlet. When released, the inlet valve head undergoes a single oscillation past the equilibrium position to a maximum open position and returns to a biased return position close to the closed position to choke the flow and produce a pressure drop across the inlet valve causing the inlet valve to close. In other embodiments, the harmonic oscillator arrangement of the inlet valve enables the uniflow engine to be reversibly operated as a uniflow compressor.

  1. Fiber bundle based endomicroscopy prototype with two collection channels for simultaneous coherent anti-Stokes Raman scattering and second harmonic generation imaging

    NASA Astrophysics Data System (ADS)

    Liu, Zhengfan; Satira, Zachary A.; Wang, Xi; Xu, Xiaoyun; Chen, Xu; Wong, Kelvin; Chen, Shufen; Xin, Jianguo; Wong, Stephen T. C.

    2014-02-01

    Label-free multiphoton imaging is promising for replacing biopsy and could offer new strategies for intraoperative or surgical applications. Coherent anti-Stokes Raman scattering (CARS) imaging could provide lipid-band contrast, and second harmonic generation (SHG) imaging is useful for imaging collagen, tendon and muscle fibers. A combination of these two imaging modalities could provide rich information and this combination has been studied by researchers to investigate diseases through microscopy imaging. The combination of these two imaging modalities in endomicroscopy imaging has been rarely investigated. In this research, a fiber bundle consisted of one excitation fiber and 18 collection fibers was developed in our endomicroscopy prototype. The 18 collection fibers were divided into two collection channels with 9 fibers in each channel. These two channels could be used together as one channel for effective signal collection or used separately for simplifying detection part of the system. Differences of collection pattern of these two channels were investigated. Collection difference of central excitation fiber and surrounding 18 fibers was also investigated, which reveals the potential ability of this system to measure forward to backward (F/B) ratio in SHG imaging. CARS imaging of mouse adipocyte and SHG imaging of mouse tail tendon were performed to demonstrate the CARS and SHG tissue imaging performance of this system. Simultaneous CARS and SHG imaging ability of this system was demonstrated by mouse tail imaging. This fiber bundle based endomicroscopy imaging prototype, offers a promising platform for constructing efficient fiber-based CARS and SHG multimodal endomicroscopes for label free intraoperative imaging applications.

  2. Model-based frequency response characterization of a digital-image analysis system for epifluorescence microscopy

    NASA Technical Reports Server (NTRS)

    Hazra, Rajeeb; Viles, Charles L.; Park, Stephen K.; Reichenbach, Stephen E.; Sieracki, Michael E.

    1992-01-01

    Consideration is given to a model-based method for estimating the spatial frequency response of a digital-imaging system (e.g., a CCD camera) that is modeled as a linear, shift-invariant image acquisition subsystem that is cascaded with a linear, shift-variant sampling subsystem. The method characterizes the 2D frequency response of the image acquisition subsystem to beyond the Nyquist frequency by accounting explicitly for insufficient sampling and the sample-scene phase. Results for simulated systems and a real CCD-based epifluorescence microscopy system are presented to demonstrate the accuracy of the method.

  3. Performance comparison of ISAR imaging method based on time frequency transforms

    NASA Astrophysics Data System (ADS)

    Xie, Chunjian; Guo, Chenjiang; Xu, Jiadong

    2013-03-01

    Inverse synthetic aperture radar (ISAR) can image the moving target, especially the target in the air, so it is important in the air defence and missile defence system. Time-frequency Transform was applied to ISAR imaging process widely. Several time frequency transforms were introduced. Noise jamming methods were analysed, and when these noise jamming were added to the echo of the ISAR receiver, the image can become blur even can't to be identify. But the effect is different to the different time frequency analysis. The results of simulation experiment show the Performance Comparison of the method.

  4. Spatial pattern separation of chemicals and frequency-independent components by terahertz spectroscopic imaging

    NASA Astrophysics Data System (ADS)

    Watanabe, Yuuki; Kawase, Kodo; Ikari, Tomofumi; Ito, Hiromasa; Ishikawa, Youichi; Minamide, Hiroaki

    2003-10-01

    We separated the component spatial patterns of frequency-dependent absorption in chemicals and frequency-independent components such as plastic, paper, and measurement noise in terahertz (THz) spectroscopic images, using known spectral curves. Our measurement system, which uses a widely tunable coherent THz-wave parametric oscillator source, can image at a specific frequency in the range 1-2 THz. The component patterns of chemicals can easily be extracted by use of the frequency-independent components. This method could be successfully used for nondestructive inspection for the detection of illegal drugs and devices of bioterrorism concealed, e.g., inside mail and packages.

  5. Few-photon-level two-dimensional infrared imaging by coincidence frequency upconversion

    NASA Astrophysics Data System (ADS)

    Huang, Kun; Gu, Xiaorong; Pan, Haifeng; E Wu, Zeng, Heping

    2012-04-01

    We demonstrate few-photon-level infrared imaging at 1040 nm by coincidence frequency upconversion with a high conversion efficiency of 33.5%. By synchronous pulse pumping at 1549 nm, the infrared object image was spectrally upconverted into the visible regime. The upconverted image was captured by a silicon electron multiplying charged coupled device without any scanning devices, thus gaining in simplicity and speed. The imaging sensitivity was improved by reducing the background noise with coincidence pulsed pumping at long wavelength.

  6. Multi-chromatic magnetic resonance imaging using frequency lock-in suppression.

    PubMed

    Chen, Yu-Wen; Hwang, Dennis W

    2015-10-01

    This study developed a multi-chromatic MR contrast using the frequency lock-in technique. An electronic feedback device that generates a specific narrow-frequency-bandwidth RF field is presented. The effects of this RF field on MR images are assessed both theoretically and experimentally. Spectroscopy and imaging experiments were performed. Frequency tuning allowed the selected spectral peak to be suppressed. Phantom tests using methanol, ethanol, and water showed different contrasts using different feedback RF field frequencies. The frequency lock-in was also found to help differentiate among the small structural variations in biological tissues. The contrast achieved in in vivo mouse brain imaging using the lock-in suppressed technique indicated a better spatial discrimination when compared with that achieved using conventional imaging methods, especially in the hippocampus region. Selective lock-in suppressed imaging is a new approach to provide frequency information in MRI; rather than determining the evolution of image contrast over time, this approach allows small susceptibility variations to be distinguished by tuning the frequency of the narrow-bandwidth lock-in RF field. A new and enhanced contrast can be achieved using this technique. PMID:26282163

  7. Evaluation of influences of frequency and amplitude on image degradation caused by satellite vibrations

    NASA Astrophysics Data System (ADS)

    Nan, Yi-Bing; Tang, Yi; Zhang, Li-Jun; Zheng, Cheng; Wang, Jing

    2015-05-01

    Satellite vibrations during exposure will lead to pixel aliasing of remote sensors, resulting in the deterioration of image quality. In this paper, we expose the problem and discuss the characteristics of satellite vibrations, and then present a pixel mixing model. The idea of mean mixing ratio (MMR) is proposed. MMR computations for different frequencies are implemented. In the mixing model, a coefficient matrix is introduced to estimate each mixed pixel. Thus, the simulation of degraded image can be performed when the vibration attitudes are known. The computation of MMR takes into consideration the influences of various frequencies and amplitudes. Therefore, the roles of these parameters played in the degradation progress are identified. Computations show that under the same vibration amplitude, the influence of vibrations fluctuates with the variation of frequency. The fluctuation becomes smaller as the frequency rises. Two kinds of vibration imaging experiments are performed: different amplitudes with the same frequency and different frequencies with the same amplitude. Results are found to be in very good agreement with the theoretical results. MMR has a better description of image quality than modulation transfer function (MTF). The influence of vibrations is determined mainly by the amplitude rather than the frequency. The influence of vibrations on image quality becomes gradually stable with the increase of frequency. Project supported by the National Basic Research Program of China (Grant No. 2013CB329202) and the Basic Industrial Technology Project of China (Grant No. J312012B002).

  8. Novel infrared image enhancement technology based on the frequency compensation approach

    NASA Astrophysics Data System (ADS)

    Qi, Yuhua; He, Rulong; Lin, Haitao

    2016-05-01

    A novel infrared image enhancement method has been proposed in this paper. Our aim is to develop a detail enhancement method which is focused on the frequency feature of the image. The proposed method is following the most popular strategy of enhancing the infrared images nowadays, but concentrating on the frequency domain. Firstly, the original image is separated by a guided image filter into detail layer and the base layer. Quite unlike the traditional methods, we use the guided image filter to eliminate most of the noise and weak signal of the scenario. Then, by a designed iteration process, the higher frequency of the scenario will be calculated back and add to the detail layer. The noise will not be enhanced because the iteration is only focused on the leftover scenario frequency. We run many tests on the raw data captured by the 320 × 256 HgCdTe cooled thermal imager, and make a comparison between our approach with the previous method of bilateral filtering digital detail enhancement and guided image filtering digital detail enhancement. Figures and analytical data show that our method is better than the previous proposed researches. Our method could effectively process the infrared image with less noise and artifacts, which has potential applications in testing, manufacturing, chemical imaging, night vision, and surveillance security.

  9. SAR imaging from partial-aperture data with frequency-band omissions

    NASA Astrophysics Data System (ADS)

    Cetin, Mujdat; Moses, Randolph L.

    2005-05-01

    We consider the problem of wide-angle SAR imaging from data with arbitrary frequency-band omissions. We propose an approach that involves composite image formation through combination of subaperture images, as well as point-enhanced, superresolution image reconstruction. This framework provides a number of desirable features including preservation of anisotropic scatterers that do not persist over the full wide-angle aperture; robustness to bandwidth limitations and frequency-band omissions; as well as a characterization of the aspect dependence of scatterers. We present experimental results based on the Air Force Research Laboratory (AFRL) "Backhoe Data Dome," demonstrating the effectiveness of the proposed approach.

  10. Experimental evidence of the theoretical spatial frequency response of cubic phase mask wavefront coding imaging systems.

    PubMed

    Somayaji, Manjunath; Bhakta, Vikrant R; Christensen, Marc P

    2012-01-16

    The optical transfer function of a cubic phase mask wavefront coding imaging system is experimentally measured across the entire range of defocus values encompassing the system's functional limits. The results are compared against mathematical expressions describing the spatial frequency response of these computational imagers. Experimental data shows that the observed modulation and phase transfer functions, available spatial frequency bandwidth and design range of this imaging system strongly agree with previously published mathematical analyses. An imaging system characterization application is also presented wherein it is shown that the phase transfer function is more robust than the modulation transfer function in estimating the strength of the cubic phase mask. PMID:22274533

  11. Millimeter-wave imaging with frequency scanning antenna and optical arrayed waveguide gratings

    NASA Astrophysics Data System (ADS)

    He, Yuntao; Yu, Guoxin; Fu, Xinyu; Jiang, Yuesong

    2012-12-01

    The principle of a novel passive millimeter-wave (MMW) imaging method using frequency scanning antenna (FSA) and arrayed waveguide grating (AWG) is analyzed theoretically. The imaging processes are divided to three stages and discussed respectively. Then the FSA with 33~ 43GHz frequency scanning range is designed carefully with a field of view of +/-25°for the MMW imaging system. An AWG of 1×24 is then simply designed with a channel spacing of 0.5GHz. The designing and simulating demonstrated the feasibility to build such an imaging system which is progressing.

  12. Multispectral imaging of tissue absorption and scattering using spatial frequency domain imaging and a computed-tomography imaging spectrometer

    PubMed Central

    Weber, Jessie R.; Cuccia, David J.; Johnson, William R.; Bearman, Gregory H.; Durkin, Anthony J.; Hsu, Mike; Lin, Alexander; Binder, Devin K.; Wilson, Dan; Tromberg, Bruce J.

    2011-01-01

    We present an approach for rapidly and quantitatively mapping tissue absorption and scattering spectra in a wide-field, noncontact imaging geometry by combining multifrequency spatial frequency domain imaging (SFDI) with a computed-tomography imaging spectrometer (CTIS). SFDI overcomes the need to spatially scan a source, and is based on the projection and analysis of periodic structured illumination patterns. CTIS provides a throughput advantage by simultaneously diffracting multiple spectral images onto a single CCD chip to gather spectra at every pixel of the image, thus providing spatial and spectral information in a single snapshot. The spatial-spectral data set was acquired 30 times faster than with our wavelength-scanning liquid crystal tunable filter camera, even though it is not yet optimized for speed. Here we demonstrate that the combined SFDI-CTIS is capable of rapid, multispectral imaging of tissue absorption and scattering in a noncontact, nonscanning platform. The combined system was validated for 36 wavelengths between 650–1000 nm in tissue simulating phantoms over a range of tissue-like absorption and scattering properties. The average percent error for the range of absorption coefficients (μa) was less than 10% from 650–800 nm, and less than 20% from 800–1000 nm. The average percent error in reduced scattering coefficients (\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{upgreek} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}\\mu_{\\rm s}^{\\prime}\\end{equation*} \\end{document}μs′) was less than 5% from 650–700 nm and less than 3% from 700–1000 nm. The SFDI-CTIS platform was applied to a mouse model of brain injury in order to demonstrate the utility of this approach in characterizing spatially and spectrally varying tissue optical properties. PMID:21280902

  13. An automatic method to determine cutoff frequency based on image power spectrum

    SciTech Connect

    Beis, J.S.; Celler, A.; Barney, J.S.

    1995-12-01

    The authors present an algorithm for automatically choosing filter cutoff frequency (F{sub c}) using the power spectrum of the projections. The method is based on the assumption that the expectation of the image power spectrum is the sum of the expectation of the blurred object power spectrum (dominant at low frequencies) plus a constant value due to Poisson noise. By considering the discrete components of the noise-dominated high-frequency spectrum as a Gaussian distribution N({mu},{sigma}), the Student t-test determines F{sub c} as the highest frequency for which the image frequency components are unlikely to be drawn from N ({mu},{sigma}). The method is general and can be applied to any filter. In this work, the authors tested the approach using the Metz restoration filter on simulated, phantom, and patient data with good results. Quantitative performance of the technique was evaluated by plotting recovery coefficient (RC) versus NMSE of reconstructed images.

  14. Experimental 0.22 THz Stepped Frequency Radar System for ISAR Imaging

    NASA Astrophysics Data System (ADS)

    Liang, Mei Yan; Zhang, Cun Lin; Zhao, Ran; Zhao, Yue Jin

    2014-09-01

    High resolution inverse synthetic aperture radar (ISAR) imaging is demonstrated by using a 0.22 THz stepped-frequency (SF) imaging radar system. The synthesis bandwidth of the terahertz (THz) SF radar is 12 GHz, which are beneficial for high resolution imaging. The resolution of ISAR image can reach centimeter-scale with the use of Range-Doppler algorithm (RDA). Results indicate that high resolution ISAR imaging is realized by using 0.22THz SF radar coupled with turntable scanning, which can provide foundations for further research on high-resolution radar image in the THz band.

  15. Major Bleeding after Percutaneous Image-Guided Biopsies: Frequency, Predictors, and Periprocedural Management

    PubMed Central

    Kennedy, Sean A.; Milovanovic, Lazar; Midia, Mehran

    2015-01-01

    Major bleeding remains an uncommon yet potentially devastating complication following percutaneous image-guided biopsy. This article reviews two cases of major bleeding after percutaneous biopsy and discusses the frequency, predictors, and periprocedural management of major postprocedural bleeding. PMID:25762845

  16. Extending the ICRF to Higher Radio Frequencies: Imaging and Source Structure

    NASA Technical Reports Server (NTRS)

    Boboltz, David A.; Fey, Alan L.; Charlot, Patrick; Fomalont, Edward B.; Lanyi, Gabor E.; Zhang, Li-Wei

    2004-01-01

    We present imaging results and source structure analysis of extragalactic radio sources observed using the Very Long Baseline Array (VLBA) at 24 GHz and 43 GHz as part of an ongoing NASA, USNO, NRAO and Bordeaux Observatory collaboration to extend the International Celestial Reference Frame (ICRF) to higher radio frequencies. The K/Q-band image database now includes images of 108 sources at 43 GHz (Q-braid) and images of 230 sources at 24 GHz (K-band). Preliminary analysis of the observations taken to date shows that the sources are generally more compact as one goes from the ICRF frequency of 8.4 GHz to 24 GHz. This result is consistent with the standard theory of compact extragalactic radio sources and suggests that reference frames defined at these higher radio frequencies will be less susceptible to the effects of intrinsic source structure than those defined at lower frequencies.

  17. Nanoscale Subsurface Imaging via Resonant Difference-Frequency Atomic Force Ultrasonic Microscopy

    NASA Technical Reports Server (NTRS)

    Cantrell, Sean A.; Cantrell, John H.; Lilehei, Peter T.

    2007-01-01

    A novel scanning probe microscope methodology has been developed that employs an ultrasonic wave launched from the bottom of a sample while the cantilever of an atomic force microscope, driven at a frequency differing from the ultrasonic frequency by the fundamental resonance frequency of the cantilever, engages the sample top surface. The nonlinear mixing of the oscillating cantilever and the ultrasonic wave in the region defined by the cantilever tip-sample surface interaction force generates difference-frequency oscillations at the cantilever fundamental resonance. The resonance-enhanced difference-frequency signals are used to create images of embedded nanoscale features.

  18. High-repetition-rate and high-photon-flux 70 eV high-harmonic source for coincidence ion imaging of gas-phase molecules.

    PubMed

    Rothhardt, Jan; Hädrich, Steffen; Shamir, Yariv; Tschnernajew, Maxim; Klas, Robert; Hoffmann, Armin; Tadesse, Getnet K; Klenke, Arno; Gottschall, Thomas; Eidam, Tino; Limpert, Jens; Tünnermann, Andreas; Boll, Rebecca; Bomme, Cedric; Dachraoui, Hatem; Erk, Benjamin; Di Fraia, Michele; Horke, Daniel A; Kierspel, Thomas; Mullins, Terence; Przystawik, Andreas; Savelyev, Evgeny; Wiese, Joss; Laarmann, Tim; Küpper, Jochen; Rolles, Daniel

    2016-08-01

    Unraveling and controlling chemical dynamics requires techniques to image structural changes of molecules with femtosecond temporal and picometer spatial resolution. Ultrashort-pulse x-ray free-electron lasers have significantly advanced the field by enabling advanced pump-probe schemes. There is an increasing interest in using table-top photon sources enabled by high-harmonic generation of ultrashort-pulse lasers for such studies. We present a novel high-harmonic source driven by a 100 kHz fiber laser system, which delivers 1011 photons/s in a single 1.3 eV bandwidth harmonic at 68.6 eV. The combination of record-high photon flux and high repetition rate paves the way for time-resolved studies of the dissociation dynamics of inner-shell ionized molecules in a coincidence detection scheme. First coincidence measurements on CH3I are shown and it is outlined how the anticipated advancement of fiber laser technology and improved sample delivery will, in the next step, allow pump-probe studies of ultrafast molecular dynamics with table-top XUV-photon sources. These table-top sources can provide significantly higher repetition rates than the currently operating free-electron lasers and they offer very high temporal resolution due to the intrinsically small timing jitter between pump and probe pulses. PMID:27505779

  19. Optical correlation of spatial-frequency-shifted images in a photorefractive BSO correlator.

    PubMed

    Tavassoli, Abtine; Becker, Michael F

    2004-03-10

    The optical cross correlation of an image with another image that was spatial-frequency shifted in one dimension was demonstrated in a photorefractive VanderLugt correlator. The first image was stored as a Fourier-transform hologram in a photorefractive Bi12SiO20 crystal (BSO) and was successively correlated with different spatial-frequency-shifted versions of a second image. We implemented the spatial-frequency shift by rotating a galvanometer mirror in an image plane, causing the Fourier transform to be shifted laterally in the BSO. We verified that the resulting operation in the BSO was an accurate complex multiplication of the shifted and the stored Fourier transforms. As many as 20 successive readouts were conducted without measurable erasure of the stored hologram. The dynamic range, saturation behavior, and other performance parameters were measured and are discussed. PMID:15046173

  20. A design of novel type superconducting magnet for super-high field functional magnetic resonance imaging by using the harmonic analysis method of magnetic vector potentials

    NASA Astrophysics Data System (ADS)

    Zu, Dong-Lin; Guo, Hua; Song, Xiao-Yu; Bao, Shang-Lian

    2002-10-01

    The approach of expanding the magnetic scalar potential in a series of Legendre polynomials is suitable for designing a conventional superconducting magnetic resonance imaging magnet of distributed solenoidal configuration. Whereas the approach of expanding the magnetic vector potential in associated Legendre harmonics is suitable for designing a single-solenoid magnet that has multiple tiers, in which each tier may have multiple layers with different winding lengths. A set of three equations to suppress some of the lowest higher-order harmonics is found. As an example, a 4T single-solenoid magnetic resonance imaging magnet with 4×6 layers of superconducting wires is designed. The degree of homogeneity in the 0.5m diameter sphere volume is better than 5.8 ppm. The same degree of homogeneity is retained after optimal integralization of turns in each correction layer. The ratio Bm/B0 in the single-solenoid magnet is 30% lower than that in the conventional six-solenoid magnet. This tolerates higher rated superconducting current in the coil. The Lorentz force of the coil in the single-solenoid system is also much lower than in the six-solenoid system. This novel type of magnet possesses significant advantage over conventional magnets, especially when used as a super-high field functional magnetic resonance imaging magnet.

  1. Final Scientific/Technical Report for DE-FG03-02NA00063 Coherent imaging of laser-plasma interactions using XUV high harmonic radiation

    SciTech Connect

    Henry Kapteyn

    2006-06-06

    The objective of this project was to develop experimental techniques for using coherent extreme-ultraviolet (EUV) radiation generated using the high-order harmonic generation technique, as an illumination source for studies of high-density plasmas relevant to the stockpile stewardship mission. In this project, we made considerable progress, including the first demonstration of imaging of dynamic processes using this coherent ultrashort pulse light. This work also stimulated considerable progress in the development of the required ultrashort EUV pulses, and in the development of new laser technologies that have been commercialized. We also demonstrated the first EUV sources that exhibit full intrinsic optical coherence. This work resulted in 12 publications.

  2. Ghost imaging with different frequencies through non-degenerated four-wave mixing.

    PubMed

    Yu, Ya; Wang, Chengyuan; Liu, Jun; Wang, Jinwen; Cao, Mingtao; Wei, Dong; Gao, Hong; Li, Fuli

    2016-08-01

    As a novel imaging method, ghost imaging has been widely explored in various fields of research, such as lensless ghost imaging, computational ghost imaging, turbulence-free ghost imaging. Recently, ghost imaging in non-degenerated system with pseudo-thermal light has been discussed theoretically, however, to our best knowledge, no experimental evidence has been proven yet. In this paper, we propose a new approach to realize ghost imaging with different frequencies, which are generated through a non-degenerated four-wave mixing(FWM) process in Rb vapor. In our experiment, by employing pseudo-thermal light as the probe beam, we found that the generated FWM signal has a strong second-order correlation with the original thermal light. On basis of that, we successfully implement non-degenerate ghost imaging, and reconstruct highly similar images of objects. PMID:27505792

  3. Quantitative modulated imaging of turbid media in the high spatial frequency domain

    NASA Astrophysics Data System (ADS)

    Lin, Weihao; Cao, Zili; Zeng, Bixin; Xu, M.

    2016-03-01

    The Spatial-frequency dependence of turbid media reflectance encodes both optical properties and depth information. The high spatial frequency domain imaging (HSFDI) can, in particular, extract key characteristics of the phase function of the scattering medium which carries the ultimate structural information of the medium. We first outline the principle of HSFDI and then present here a compact optical configuration integrating the modulated illumination and imaging systems, facilitating quantitative wide-field optical properties mapping at high spatial frequencies. The performance of HSFDI is assessed on both tissue phantoms and in vivo.

  4. Appearance-based exercise motivation moderates the relationship between exercise frequency and positive body image.

    PubMed

    Homan, Kristin J; Tylka, Tracy L

    2014-03-01

    Individuals with a positive body image appreciate their bodies, hold an internal perspective of their bodies, and are satisfied with the functionality of their bodies. Research shows that positive body image is more complex than the absence of body dissatisfaction. Although exercise reduces women's body dissatisfaction, very little research has explored how, or even whether, exercise is associated with positive body image. Therefore, we examined whether exercise frequency was positively related to three aspects of positive body image (body appreciation, internal body orientation, and functional body satisfaction) among 321 college women. Appearance-based exercise motivation (the extent exercise is pursued to influence weight or shape) was hypothesized to moderate these associations. Hierarchical moderated regression analyses showed that exercise frequency was related to higher positive body image, but high levels of appearance-based exercise motivation weakened these relationships. Thus, messages promoting exercise need to de-emphasize weight loss and appearance for positive body image. PMID:24529336

  5. Optimal geometries and harmonic vibrational frequencies of the global minima of water clusters (H2O)n, n = 2-6, and several hexamer local minima at the CCSD(T) level of theory.

    PubMed

    Miliordos, Evangelos; Aprà, Edoardo; Xantheas, Sotiris S

    2013-09-21

    We report the first optimum geometries and harmonic vibrational frequencies for the ring pentamer and several water hexamer (prism, cage, cyclic and two book) at the coupled-cluster including single, double, and full perturbative triple excitations (CCSD(T))/aug-cc-pVDZ level of theory. All five examined hexamer isomer minima previously reported by Møller-Plesset perturbation theory (MP2) are also minima on the CCSD(T) potential energy surface (PES). In addition, all CCSD(T) minimum energy structures for the n = 2-6 cluster isomers are quite close to the ones previously obtained by MP2 on the respective PESs, as confirmed by a modified Procrustes analysis that quantifies the difference between any two cluster geometries. The CCSD(T) results confirm the cooperative effect of the homodromic ring networks (systematic contraction of the nearest-neighbor (nn) intermolecular separations with cluster size) previously reported by MP2, albeit with O-O distances shorter by ~0.02 Å, indicating that MP2 overcorrects this effect. The harmonic frequencies at the minimum geometries were obtained by the double differentiation of the CCSD(T) energy using an efficient scheme based on internal coordinates that reduces the number of required single point energy evaluations by ~15% when compared to the corresponding double differentiation using Cartesian coordinates. Negligible differences between MP2 and CCSD(T) frequencies are found for the librational modes, while uniform increases of ~15 and ~25 cm(-1) are observed for the bending and "free" OH harmonic frequencies. The largest differences between CCSD(T) and MP2 are observed for the harmonic hydrogen bonded frequencies, for which the former produces larger absolute values than the latter. Their CCSD(T) redshifts from the monomer values (Δω) are smaller than the MP2 ones, due to the fact that CCSD(T) produces shorter elongations (ΔR) of the respective hydrogen bonded OH lengths from the monomer value with respect to MP2. Both

  6. Optimal geometries and harmonic vibrational frequencies of the global minima of water clusters (H2O)n, n = 2-6, and several hexamer local minima at the CCSD(T) level of theory

    NASA Astrophysics Data System (ADS)

    Miliordos, Evangelos; Aprà, Edoardo; Xantheas, Sotiris S.

    2013-09-01

    We report the first optimum geometries and harmonic vibrational frequencies for the ring pentamer and several water hexamer (prism, cage, cyclic and two book) at the coupled-cluster including single, double, and full perturbative triple excitations (CCSD(T))/aug-cc-pVDZ level of theory. All five examined hexamer isomer minima previously reported by Møller-Plesset perturbation theory (MP2) are also minima on the CCSD(T) potential energy surface (PES). In addition, all CCSD(T) minimum energy structures for the n = 2-6 cluster isomers are quite close to the ones previously obtained by MP2 on the respective PESs, as confirmed by a modified Procrustes analysis that quantifies the difference between any two cluster geometries. The CCSD(T) results confirm the cooperative effect of the homodromic ring networks (systematic contraction of the nearest-neighbor (nn) intermolecular separations with cluster size) previously reported by MP2, albeit with O-O distances shorter by ˜0.02 Å, indicating that MP2 overcorrects this effect. The harmonic frequencies at the minimum geometries were obtained by the double differentiation of the CCSD(T) energy using an efficient scheme based on internal coordinates that reduces the number of required single point energy evaluations by ˜15% when compared to the corresponding double differentiation using Cartesian coordinates. Negligible differences between MP2 and CCSD(T) frequencies are found for the librational modes, while uniform increases of ˜15 and ˜25 cm-1 are observed for the bending and "free" OH harmonic frequencies. The largest differences between CCSD(T) and MP2 are observed for the harmonic hydrogen bonded frequencies, for which the former produces larger absolute values than the latter. Their CCSD(T) redshifts from the monomer values (Δω) are smaller than the MP2 ones, due to the fact that CCSD(T) produces shorter elongations (ΔR) of the respective hydrogen bonded OH lengths from the monomer value with respect to MP2

  7. Harmonic generation at high intensities

    SciTech Connect

    Schafer, K.J.; Krause, J.L.; Kulander, K.C.

    1993-06-01

    Atomic electrons subject to intense laser fields can absorb many photons, leading either to multiphoton ionization or the emission of a single, energetic photon which can be a high multiple of the laser frequency. The latter process, high-order harmonic generation, has been observed experimentally using a range of laser wavelengths and intensities over the past several years. Harmonic generation spectra have a generic form: a steep decline for the low order harmonics, followed by a plateau extending to high harmonic order, and finally an abrupt cutoff beyond which no harmonics are discernible. During the plateau the harmonic production is a very weak function of the process order. Harmonic generation is a promising source of coherent, tunable radiation in the XUV to soft X-ray range which could have a variety of scientific and possibly technological applications. Its conversion from an interesting multiphoton phenomenon to a useful laboratory radiation source requires a complete understanding of both its microscopic and macroscopic aspects. We present some recent results on the response of single atoms at intensities relevant to the short pulse experiments. The calculations employ time-dependent methods, which we briefly review in the next section. Following that we discuss the behavior of the harmonics as a function of laser intensity. Two features are notable: the slow scaling of the harmonic intensities with laser intensity, and the rapid variation in the phase of the individual harmonics with respect to harmonic order. We then give a simple empirical formula that predicts the extent of the plateau for a given ionization potential, wavelength and intensity.

  8. Three-dimensional speckle imaging employing a frequency-locked tunable diode laser

    NASA Astrophysics Data System (ADS)

    Cannon, Bret D.; Bernacki, Bruce E.; Schiffern, John T.; Mendoza, Albert

    2015-09-01

    We describe a high accuracy frequency stepping method for a tunable diode laser to improve a three dimensional (3D) imaging approach based upon interferometric speckle imaging. The approach, modeled after Takeda, exploits tuning an illumination laser in frequency as speckle interferograms of the object (specklegrams) are acquired at each frequency in a Michelson interferometer. The resulting 3D hypercube of specklegrams encode spatial information in the x-y plane of each image with laser tuning arrayed along its z-axis. The specklegrams are processed by Fast Fourier Transformation (FFT) along the z-axis of the hypercube and the center of the peak in the resulting power spectrum for each pixel encodes its surface height. Alternatively, Takeda's method can be followed which uses the phase of the FFT, unwraps it, and determines the surface height encoded in the slope of a line fitted to the phase. Wraparound of modulations above the Nyquist limit results in ambiguity in the optical path difference (OPD) between test and reference surfaces. Wraparound also amplifies measurement noise caused by errors and jitter in frequency stepping the illumination laser. By locking the laser frequency to successive cavity modes of a reference confocal interferometer, tuning is precisely controlled resulting in dramatically improved imaging quality/. We present laboratory data of before and after results showing enhanced 3D imaging resulting from precise laser frequency control.

  9. Preliminary investigation of the frequency response and distortion properties of nonlinear image processing algorithms

    NASA Astrophysics Data System (ADS)

    Wells, Jered R.; Dobbins, James T.

    2013-03-01

    Assessment of the resolution properties of nonlinear imaging systems is a useful but challenging task. While the modulation transfer function (MTF) fully describes contrast resolution as a function of spatial frequency for linear systems, an equivalent metric does not exist for systems with significant nonlinearity. Therefore, this preliminary investigation attempts to classify and quantify the amount of scaling and distortion imposed on a given image signal as the result of a nonlinear process (nonlinear image processing algorithm). As a proof-of-concept, a median filter is assessed in terms of its principle frequency response (PFR) and distortion response (DR) functions. These metrics are derived in frequency space using a sinusoidal basis function, and it is shown that, for a narrow-band sinusoidal input signal, the scaling and distortion properties of the nonlinear filter are described exactly by PFR and DR, respectively. The use of matched sinusoidal basis and input functions accurately reveals the frequency response to long linear structures of different scale. However, when more complex (multi-band) input signals are considered, PFR and DR fail to adequately characterize the frequency response due to nonlinear interaction effects between different frequency components during processing. Overall, the results reveal the context-dependent nature of nonlinear image processing algorithm performance, and they emphasize the importance of the basis function choice in algorithm assessment. In the future, more complex forms of nonlinear systems analysis may be necessary to fully characterize the frequency response properties of nonlinear algorithms in a context-dependent manner.

  10. Frequency-domain ultrasound waveform tomography breast attenuation imaging

    NASA Astrophysics Data System (ADS)

    Sandhu, Gursharan Yash Singh; Li, Cuiping; Roy, Olivier; West, Erik; Montgomery, Katelyn; Boone, Michael; Duric, Neb

    2016-04-01

    Ultrasound waveform tomography techniques have shown promising results for the visualization and characterization of breast disease. By using frequency-domain waveform tomography techniques and a gradient descent algorithm, we have previously reconstructed the sound speed distributions of breasts of varying densities with different types of breast disease including benign and malignant lesions. By allowing the sound speed to have an imaginary component, we can model the intrinsic attenuation of a medium. We can similarly recover the imaginary component of the velocity and thus the attenuation. In this paper, we will briefly review ultrasound waveform tomography techniques, discuss attenuation and its relations to the imaginary component of the sound speed, and provide both numerical and ex vivo examples of waveform tomography attenuation reconstructions.

  11. High precision spectroscopy and imaging in THz frequency range

    NASA Astrophysics Data System (ADS)

    Vaks, Vladimir L.

    2014-03-01

    Application of microwave methods for development of the THz frequency range has resulted in elaboration of high precision THz spectrometers based on nonstationary effects. The spectrometers characteristics (spectral resolution and sensitivity) meet the requirements for high precision analysis. The gas analyzers, based on the high precision spectrometers, have been successfully applied for analytical investigations of gas impurities in high pure substances. These investigations can be carried out both in absorption cell and in reactor. The devices can be used for ecological monitoring, detecting the components of chemical weapons and explosive in the atmosphere. The great field of THz investigations is the medicine application. Using the THz spectrometers developed one can detect markers for some diseases in exhaled air.

  12. Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis

    NASA Astrophysics Data System (ADS)

    Yeh, Kevin; Bhargava, Rohit

    2016-03-01

    Infrared (IR) spectroscopic imaging is an emerging modality for biological tissue analysis that has traditionally employed an interferometer for spectral discrimination. Recent technology developments have made discrete frequency sources, both lasers and filters, practical for imaging. The use of quantum cascade lasers in particular, presents new opportunities as well as challenges. Here we describe results from a novel point scanning confocal IR microscope and demonstrate the performance imaging several important spectral bands of lung tissue. Results show the possibility of discrete frequency (DF) absorbance measurements with RMS noise levels down to 0.34 mAU in 0.25 ms.

  13. Time-of-flight detection of ultra-cold atoms using resonant frequency modulation imaging.

    PubMed

    Hardman, K S; Wigley, P B; Everitt, P J; Manju, P; Kuhn, C C N; Robins, N P

    2016-06-01

    Resonant frequency modulation imaging is used to detect free falling ultra-cold atoms. A theoretical comparison of fluorescence imaging (FI) and frequency modulation imaging (FMI) is made, indicating that for low optical depth clouds, FMI accomplished a higher signal-to-noise ratio under conditions necessary for a 200 μm spatially resolved atom interferometer. A 750 ms time-of-flight measurement reveals near atom shot-noise limited number measurements of 2×106 Bose-condensed Rb87 atoms. The detection system is applied to high precision spinor BEC based atom interferometer. PMID:27244400

  14. Elimination of depth degeneracy in optical frequency-domain imaging through polarization-based optical demodulation.

    PubMed

    Vakoc, B J; Yun, S H; Tearney, G J; Bouma, B E

    2006-02-01

    A novel optical frequency-domain imaging system is demonstrated that employs a passive optical demodulation circuit and a chirped digital acquisition clock derived from a voltage-controlled oscillator. The demodulation circuit allows the separation of signals from positive and negative depths to better than 50 dB, thereby eliminating depth degeneracy and doubling the imaging depth range. Our system design is compatible with dual-balanced and polarization-diverse detection, important techniques in the practical biomedical application of optical frequency-domain imaging. PMID:16480209

  15. Noncontact imaging of burn depth and extent in a porcine model using spatial frequency domain imaging

    PubMed Central

    Mazhar, Amaan; Saggese, Steve; Pollins, Alonda C.; Cardwell, Nancy L.; Nanney, Lillian; Cuccia, David J.

    2014-01-01

    Abstract. The standard of care for clinical assessment of burn severity and extent lacks a quantitative measurement. In this work, spatial frequency domain imaging (SFDI) was used to measure 48 thermal burns of graded severity (superficial partial, deep partial, and full thickness) in a porcine model. Functional (total hemoglobin and tissue oxygen saturation) and structural parameters (tissue scattering) derived from the SFDI measurements were monitored over 72 h for each burn type and compared to gold standard histological measurements of burn depth. Tissue oxygen saturation (stO2) and total hemoglobin (ctHbT) differentiated superficial partial thickness burns from more severe burn types after 2 and 72 h, respectively (p<0.01), but were unable to differentiate deep partial from full thickness wounds in the first 72 h. Tissue scattering parameters separated superficial burns from all burn types immediately after injury (p<0.01), and separated all three burn types from each other after 24 h (p<0.01). Tissue scattering parameters also showed a strong negative correlation to histological burn depth as measured by vimentin immunostain (r2>0.89). These results show promise for the use of SFDI-derived tissue scattering as a correlation to burn depth and the potential to assess burn depth via a combination of SFDI functional and structural parameters. PMID:25147961

  16. Axial standing-wave illumination frequency-domain imaging (SWIF)

    PubMed Central

    Judkewitz, Benjamin; Yang, Changhuei

    2014-01-01

    Despite their tremendous contribution to biomedical research and diagnosis, conventional spatial sampling techniques such as wide-field, point scanning or selective plane illumination microscopy face inherent limiting trade-offs between spatial resolution, field-of-view, phototoxicity and recording speed. Several of these trade-offs are the result of spatial sampling with diffracting beams. Here, we introduce a new strategy for fluorescence imaging, SWIF, which instead encodes the axial profile of a sample in the Fourier domain. We demonstrate how this can be achieved with propagation-invariant illumination patterns that extend over several millimeters and robustly propagate through layers of varying refractive index. This enabled us to image a lateral field-of-view of 0.8 mm x 1.5 mm with an axial resolution of 2.4 µm – greatly exceeding the lateral field-of-view of conventional illumination techniques (~100 µm) at comparable resolution. Thus, SWIF allowed us to surpass the limitations of diffracting illumination beams and untangle lateral field-of-view from resolution. PMID:24921798

  17. Limitations on squeezing and formation of the superposition of two macroscopically distinguishable states at fundamental frequency in the process of second harmonic generation

    NASA Technical Reports Server (NTRS)

    Nikitin, S. P.; Masalov, A. V.

    1992-01-01

    The results of numerical simulations of quantum state evolution in the process of second harmonic generation (SHG) are discussed. It is shown that at a particular moment of time in the fundamental mode initially coherent state turns into a superposition of two macroscopically distinguished states. The question of whether this superposition exhibits quantum interference is analyzed.

  18. Harmonically excited orbital variations

    SciTech Connect

    Morgan, T.

    1985-08-06

    Rephrasing the equations of motion for orbital maneuvers in terms of Lagrangian generalized coordinates instead of Newtonian rectangular cartesian coordinates can make certain harmonic terms in the orbital angular momentum vector more readily apparent. In this formulation the equations of motion adopt the form of a damped harmonic oscillator when torques are applied to the orbit in a variationally prescribed manner. The frequencies of the oscillator equation are in some ways unexpected but can nonetheless be exploited through resonant forcing functions to achieve large secular variations in the orbital elements. Two cases are discussed using a circular orbit as the control case: (1) large changes in orbital inclination achieved by harmonic excitation rather than one impulsive velocity change, and (2) periodic and secular changes to the longitude of the ascending node using both stable and unstable excitation strategies. The implications of these equations are also discussed for both artificial satellites and natural satellites. For the former, two utilitarian orbits are suggested, each exploiting a form of harmonic excitation. 5 refs.

  19. Multipixel system for gigahertz frequency-domain optical imaging of finger joints

    NASA Astrophysics Data System (ADS)

    Netz, Uwe J.; Beuthan, Jürgen; Hielscher, Andreas H.

    2008-03-01

    Frequency-domain optical imaging systems have shown great promise for characterizing blood oxygenation, hemodynamics, and other physiological parameters in human and animal tissues. However, most of the frequency domain systems presented so far operate with source modulation frequencies below 150MHz. At these low frequencies, their ability to provide accurate data for small tissue geometries such as encountered in imaging of finger joints or rodents is limited. Here, we present a new system that can provide data up to 1GHz using an intensity modulated charged coupled device camera. After data processing, the images show the two-dimensional distribution of amplitude and phase of the light modulation on the finger surface. The system performance was investigated and test measurements on optical tissue phantoms were taken to investigate whether higher frequencies yield better signal-to-noise ratios (SNRs). It could be shown that local changes in optical tissue properties, as they appear in the initial stages of rheumatoid arthritis in a finger joint, are detectable by simple image evaluation, with the range of modulation frequency around 500MHz proving to yield the highest SNR.

  20. The analysis of frequency domain characteristics of emotional images in eye-tracking experiment

    NASA Astrophysics Data System (ADS)

    Fan, Boqiang; Ma, Huimin; Wang, Xiang

    2015-07-01

    Although recently eye-tracking method has been introduced into behavioral experiments based on dot-probe paradigm, some characteristics in eye-tracking data do not draw as much attention as traditional characteristics like reaction time. It is also necessary to associate eye-tracking data to characteristics of images shown in experiments. In this research, new variables, such as fixation length, times of fixation and times of eye movement, in eye-tracking data were extracted from a behavioral experiment based on dot probe paradigm. They were analyzed and compared to traditional reaction time. After the analysis of positive and negative scenery images, parameters such as hue frequency spectrum PAR (Peak to Average Ratio) were extracted and showed difference between negative and positive images. These parameters of emotional images could discriminate scenery images according to their emotions in an SVM classifier well. Besides, it was found that images' hue frequency spectrum PAR is obviously relevant to eye-tracking statistics. When the dot was on the negative side, negative images' hue frequency spectrum PAR and horizontal eye-jumps confirmed to hyperbolic distribution, while that of positive images was linear with horizontal eye-jumps. The result could help to explain the mechanism of human's attention and boost the study in computer vision.